LOCUS       DAZPCS010000001        72983 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZPCS010000001 DAZPCS010000000
VERSION     DAZPCS010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 72983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72983
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.001"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..209)
                     /locus_tag="ACHUQ7_000001"
     CDS             complement(<1..209)
                     /locus_tag="ACHUQ7_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR1133601.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ACHUQ7_000002"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ACHUQ7_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFR1133602.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRVGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ACHUQ7_000003"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ACHUQ7_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFR1133603.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ACHUQ7_000004"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ACHUQ7_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFR1133604.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ACHUQ7_000005"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ACHUQ7_000005"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFR1133605.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ACHUQ7_000006"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ACHUQ7_000006"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFR1133606.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ACHUQ7_000007"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ACHUQ7_000007"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFR1133607.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /locus_tag="ACHUQ7_000008"
     CDS             complement(4598..5320)
                     /locus_tag="ACHUQ7_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR1133608.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ACHUQ7_000009"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ACHUQ7_000009"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR1133609.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKASFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /locus_tag="ACHUQ7_000010"
     CDS             6520..6738
                     /locus_tag="ACHUQ7_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFR1133610.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVAAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ACHUQ7_000011"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ACHUQ7_000011"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFR1133611.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /locus_tag="ACHUQ7_000012"
     CDS             complement(7472..7672)
                     /locus_tag="ACHUQ7_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFR1133612.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVHKDEQVIGIFHPD"
     gene            complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="ACHUQ7_000013"
                     /pseudo
     CDS             complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="ACHUQ7_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
     gene            complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="ACHUQ7_000014"
     CDS             complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="ACHUQ7_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFR1133613.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10101..12014
                     /locus_tag="ACHUQ7_000015"
     CDS             10101..12014
                     /locus_tag="ACHUQ7_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR1133614.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12014..13036
                     /locus_tag="ACHUQ7_000016"
     CDS             12014..13036
                     /locus_tag="ACHUQ7_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFR1133615.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13030..13248
                     /locus_tag="ACHUQ7_000017"
     CDS             13030..13248
                     /locus_tag="ACHUQ7_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFR1133616.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13302..14171
                     /locus_tag="ACHUQ7_000018"
     CDS             13302..14171
                     /locus_tag="ACHUQ7_000018"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFR1133617.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14226..14630)
                     /locus_tag="ACHUQ7_000019"
     CDS             complement(14226..14630)
                     /locus_tag="ACHUQ7_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFR1133618.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            14932..15564
                     /gene="crp"
                     /locus_tag="ACHUQ7_000020"
     CDS             14932..15564
                     /gene="crp"
                     /locus_tag="ACHUQ7_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFR1133619.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15615..17705
                     /locus_tag="ACHUQ7_000021"
     CDS             15615..17705
                     /locus_tag="ACHUQ7_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFR1133620.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPESYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17772..18992)
                     /gene="argD"
                     /locus_tag="ACHUQ7_000022"
                     /gene_synonym="dapC"
     CDS             complement(17772..18992)
                     /gene="argD"
                     /locus_tag="ACHUQ7_000022"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFR1133621.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19078..19641)
                     /gene="pabA"
                     /locus_tag="ACHUQ7_000023"
     CDS             complement(19078..19641)
                     /gene="pabA"
                     /locus_tag="ACHUQ7_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFR1133622.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(19673..20275)
                     /locus_tag="ACHUQ7_000024"
     CDS             complement(19673..20275)
                     /locus_tag="ACHUQ7_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFR1133623.1"
                     /translation="MSDKFGEGRDLYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20265..20432)
                     /locus_tag="ACHUQ7_000025"
     CDS             complement(20265..20432)
                     /locus_tag="ACHUQ7_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFR1133624.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20537..21118)
                     /gene="ppiA"
                     /locus_tag="ACHUQ7_000026"
                     /gene_synonym="rotA"
     CDS             complement(20537..21118)
                     /gene="ppiA"
                     /locus_tag="ACHUQ7_000026"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFR1133625.1"
                     /translation="MFKSTLAAMAAVFALSALSALSPAAMAAKGDPHVLLTTSAGNIE
                     LELDKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKN
                     EADNGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMD
                     VADKISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21389..22569
                     /gene="tsgA"
                     /locus_tag="ACHUQ7_000027"
                     /pseudo
     CDS             21389..22569
                     /gene="tsgA"
                     /locus_tag="ACHUQ7_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
     gene            22831..25374
                     /gene="nirB"
                     /locus_tag="ACHUQ7_000028"
     CDS             22831..25374
                     /gene="nirB"
                     /locus_tag="ACHUQ7_000028"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFR1133626.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25371..25697
                     /gene="nirD"
                     /locus_tag="ACHUQ7_000029"
     CDS             25371..25697
                     /gene="nirD"
                     /locus_tag="ACHUQ7_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFR1133627.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDKQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25823..26629
                     /gene="nirC"
                     /locus_tag="ACHUQ7_000030"
     CDS             25823..26629
                     /gene="nirC"
                     /locus_tag="ACHUQ7_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFR1133628.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26648..28021
                     /gene="cysG"
                     /locus_tag="ACHUQ7_000031"
     CDS             26648..28021
                     /gene="cysG"
                     /locus_tag="ACHUQ7_000031"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFR1133629.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28268..28435
                     /locus_tag="ACHUQ7_000032"
     CDS             28268..28435
                     /locus_tag="ACHUQ7_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFR1133630.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            28730..30067
                     /gene="frlA"
                     /locus_tag="ACHUQ7_000033"
                     /pseudo
     CDS             28730..30067
                     /gene="frlA"
                     /locus_tag="ACHUQ7_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            30088..31110
                     /gene="frlB"
                     /locus_tag="ACHUQ7_000034"
     CDS             30088..31110
                     /gene="frlB"
                     /locus_tag="ACHUQ7_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HFR1133631.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31160..31990
                     /gene="frlC"
                     /locus_tag="ACHUQ7_000035"
     CDS             31160..31990
                     /gene="frlC"
                     /locus_tag="ACHUQ7_000035"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HFR1133632.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAIDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYSTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            31987..32772
                     /gene="frlD"
                     /locus_tag="ACHUQ7_000036"
     CDS             31987..32772
                     /gene="frlD"
                     /locus_tag="ACHUQ7_000036"
                     /EC_number="2.7.1.218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFR1133633.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            32872..33603
                     /gene="frlR"
                     /locus_tag="ACHUQ7_000037"
                     /gene_synonym="yhfR"
     CDS             32872..33603
                     /gene="frlR"
                     /locus_tag="ACHUQ7_000037"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HFR1133634.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKSTK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(33763..34848)
                     /locus_tag="ACHUQ7_000038"
     CDS             complement(33763..34848)
                     /locus_tag="ACHUQ7_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFR1133635.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     SLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(34860..36164)
                     /locus_tag="ACHUQ7_000039"
     CDS             complement(34860..36164)
                     /locus_tag="ACHUQ7_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HFR1133636.1"
                     /translation="MDLYIQIIVVACLTGMTSLLEHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(36176..36529)
                     /locus_tag="ACHUQ7_000040"
     CDS             complement(36176..36529)
                     /locus_tag="ACHUQ7_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HFR1133637.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(36540..37418)
                     /locus_tag="ACHUQ7_000041"
     CDS             complement(36540..37418)
                     /locus_tag="ACHUQ7_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HFR1133638.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(37415..38641)
                     /locus_tag="ACHUQ7_000042"
     CDS             complement(37415..38641)
                     /locus_tag="ACHUQ7_000042"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFR1133639.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLSVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(38642..39805)
                     /locus_tag="ACHUQ7_000043"
     CDS             complement(38642..39805)
                     /locus_tag="ACHUQ7_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HFR1133640.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRVQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLEA"
     gene            complement(39889..40251)
                     /locus_tag="ACHUQ7_000044"
     CDS             complement(39889..40251)
                     /locus_tag="ACHUQ7_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFR1133641.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQELLKEFALEVNPCEE
                     GYFLANLYGLWMAANEEV"
     gene            complement(40268..41173)
                     /gene="yhfZ"
                     /locus_tag="ACHUQ7_000045"
     CDS             complement(40268..41173)
                     /gene="yhfZ"
                     /locus_tag="ACHUQ7_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HFR1133642.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(41443..42447)
                     /gene="trpS"
                     /locus_tag="ACHUQ7_000046"
     CDS             complement(41443..42447)
                     /gene="trpS"
                     /locus_tag="ACHUQ7_000046"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFR1133643.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFGYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(42440..43198)
                     /gene="gph"
                     /locus_tag="ACHUQ7_000047"
     CDS             complement(42440..43198)
                     /gene="gph"
                     /locus_tag="ACHUQ7_000047"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFR1133644.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(43191..43868)
                     /gene="rpe"
                     /locus_tag="ACHUQ7_000048"
     CDS             complement(43191..43868)
                     /gene="rpe"
                     /locus_tag="ACHUQ7_000048"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFR1133645.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(43886..44722)
                     /gene="dam"
                     /locus_tag="ACHUQ7_000049"
     CDS             complement(43886..44722)
                     /gene="dam"
                     /locus_tag="ACHUQ7_000049"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFR1133646.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMERADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(44829..46121)
                     /gene="damX"
                     /locus_tag="ACHUQ7_000050"
     CDS             complement(44829..46121)
                     /gene="damX"
                     /locus_tag="ACHUQ7_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFR1133647.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGK
                     TAGNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVS
                     GVYASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(46213..47301)
                     /gene="aroB"
                     /locus_tag="ACHUQ7_000051"
     CDS             complement(46213..47301)
                     /gene="aroB"
                     /locus_tag="ACHUQ7_000051"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFR1133648.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTSET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(47358..47879)
                     /gene="aroK"
                     /locus_tag="ACHUQ7_000052"
     CDS             complement(47358..47879)
                     /gene="aroK"
                     /locus_tag="ACHUQ7_000052"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFR1133649.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(48280..49518)
                     /gene="hofQ"
                     /locus_tag="ACHUQ7_000053"
     CDS             complement(48280..49518)
                     /gene="hofQ"
                     /locus_tag="ACHUQ7_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFR1133650.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QESEIPYQVSSGESGATSVEFKEAVLGMEVMPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(49430..49836)
                     /gene="hofP"
                     /locus_tag="ACHUQ7_000054"
                     /pseudo
     CDS             complement(49430..49836)
                     /gene="hofP"
                     /locus_tag="ACHUQ7_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
     gene            complement(49826..50266)
                     /gene="hofO"
                     /locus_tag="ACHUQ7_000055"
     CDS             complement(49826..50266)
                     /gene="hofO"
                     /locus_tag="ACHUQ7_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFR1133651.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLEMPHEG"
     gene            complement(50250..50789)
                     /gene="hofN"
                     /locus_tag="ACHUQ7_000056"
     CDS             complement(50250..50789)
                     /gene="hofN"
                     /locus_tag="ACHUQ7_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFR1133652.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(50789..51568)
                     /gene="hofM"
                     /locus_tag="ACHUQ7_000057"
     CDS             complement(50789..51568)
                     /gene="hofM"
                     /locus_tag="ACHUQ7_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFR1133653.1"
                     /translation="MTFKIWQIGLHLQQQEAAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            51688..54240
                     /gene="mrcA"
                     /locus_tag="ACHUQ7_000058"
     CDS             51688..54240
                     /gene="mrcA"
                     /locus_tag="ACHUQ7_000058"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFR1133654.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTMMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(54405..54965)
                     /gene="nudE"
                     /locus_tag="ACHUQ7_000059"
     CDS             complement(54405..54965)
                     /gene="nudE"
                     /locus_tag="ACHUQ7_000059"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFR1133655.1"
                     /translation="MNKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            55285..57420
                     /gene="igaA"
                     /locus_tag="ACHUQ7_000060"
     CDS             55285..57420
                     /gene="igaA"
                     /locus_tag="ACHUQ7_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFR1133656.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTEASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            57485..58153
                     /gene="yrfG"
                     /locus_tag="ACHUQ7_000061"
     CDS             57485..58153
                     /gene="yrfG"
                     /locus_tag="ACHUQ7_000061"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFR1133657.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            58164..58565
                     /gene="hslR"
                     /locus_tag="ACHUQ7_000062"
     CDS             58164..58565
                     /gene="hslR"
                     /locus_tag="ACHUQ7_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFR1133658.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            58590..59459
                     /gene="hslO"
                     /locus_tag="ACHUQ7_000063"
     CDS             58590..59459
                     /gene="hslO"
                     /locus_tag="ACHUQ7_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFR1133659.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADP"
     gene            complement(59603..61361)
                     /locus_tag="ACHUQ7_000064"
                     /pseudo
     CDS             complement(59603..61361)
                     /locus_tag="ACHUQ7_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
     gene            61740..63362
                     /gene="pckA"
                     /locus_tag="ACHUQ7_000065"
     CDS             61740..63362
                     /gene="pckA"
                     /locus_tag="ACHUQ7_000065"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFR1133660.1"
                     /translation="MRVNNGLPPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(63438..64790)
                     /gene="envZ"
                     /locus_tag="ACHUQ7_000066"
     CDS             complement(63438..64790)
                     /gene="envZ"
                     /locus_tag="ACHUQ7_000066"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFR1133661.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(64787..65506)
                     /gene="ompR"
                     /locus_tag="ACHUQ7_000067"
     CDS             complement(64787..65506)
                     /gene="ompR"
                     /locus_tag="ACHUQ7_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFR1133662.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            65734..66210
                     /gene="greB"
                     /locus_tag="ACHUQ7_000068"
     CDS             65734..66210
                     /gene="greB"
                     /locus_tag="ACHUQ7_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFR1133663.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANWYVNAIEYVKP"
     gene            66307..68628
                     /locus_tag="ACHUQ7_000069"
     CDS             66307..68628
                     /locus_tag="ACHUQ7_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFR1133664.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDATLLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     SVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            69066..69293
                     /gene="feoA"
                     /locus_tag="ACHUQ7_000070"
     CDS             69066..69293
                     /gene="feoA"
                     /locus_tag="ACHUQ7_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFR1133665.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            69310..71631
                     /gene="feoB"
                     /locus_tag="ACHUQ7_000071"
     CDS             69310..71631
                     /gene="feoB"
                     /locus_tag="ACHUQ7_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFR1133666.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDATLARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFNVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGNGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVAIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            71631..71867
                     /gene="feoC"
                     /locus_tag="ACHUQ7_000072"
     CDS             71631..71867
                     /gene="feoC"
                     /locus_tag="ACHUQ7_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFR1133667.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            72070..72957
                     /gene="rpnA"
                     /locus_tag="ACHUQ7_000073"
     CDS             72070..72957
                     /gene="rpnA"
                     /locus_tag="ACHUQ7_000073"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HFR1133668.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESRSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAASRLAP"
BASE COUNT        17592 a        19781 c        18455 g        17155 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gatgatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt caggccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcattccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccacgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaaga gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcaaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagactca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag atcgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcgcttgcc
     4441 ggtgaggtca gttggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 ttatggcctt ctgctatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctgaaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5581 actagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttctttaag agagttttcc
     6121 atgtaacgac ccagcgaagc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gatgctttgc tgtctgcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agtagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tggccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccactacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctggtcagt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc actaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gtgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tgatgtccgc
     7561 acttaacaca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgcg
     7621 cctgacaggc cgggcatttt gcgcccgcta taaaacgttt tcggattgcc atccttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtacg gtatcagcat
     7801 gcatcgggat taactcccgc agcattcgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgtgcctg atgcggatgc atgccaagcg tgaccagcgt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 catatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgctaaact
     8341 ggcgggatag ccatttatcc accagcttca tcagcaacgg cgccatctgg tcgccctgga
     8401 ataaacgttg tgaagaagcg gtagaaaaga ggacaaaggc aaactcaccg ccctgactca
     8461 acacgccagc aaactgcatc cgctctgagc tacgcacgcc atacaatcgc gccagcagat
     8521 acagcacgag aattttcacc gccaccagca caaccacgct tatcactacc cacaacagat
     8581 gggtataaag caccccgagg ttgagcgaca tgccgacaga gataaagaac aaaccgagca
     8641 gcaagccttt gaagggatcg atagccgttt ccagttcatg gcgatattca ctttccgcca
     8701 gcagcacgcc cgcaataaat gtaccgagcg ccatcgacag ccccagcgca tccataaaca
     8761 atgcggaacc caacaccagc agcagcgtcg cggcggtgaa cacttcccgc acgccagaag
     8821 ctgcaataaa gcggaatacc ggacgcagta aatagcgccc accaatcagc atgccgacaa
     8881 actccagcac cttcatgccg atcttcatcc agtcgaaatg ttcgtctgcc gaccccgcca
     8941 acaacggcac taacgccagt gctgggatca ctgccagatc ctgaaacagc agaaccgaaa
     9001 acccgagctg gccggattcg ctgcgattca tccctttctc acgcatcaat tgcaacgcca
     9061 ttgcagttga agacatcgca aagccaatgc caccgaccac cgccgcctgc caggcgaaat
     9121 ccgtcagcat caataatccc gccagcaacg ccgcgcttaa cagcacctgt gccgcgccta
     9181 cgccaaaaat cgaacgccgc agttgccaaa gtttggaggg attcaactca aggccgatga
     9241 taaacatcag gaataccacg ccgagttccg aaaagtggag gatctcatcg acgtcgctaa
     9301 taaaccccag cccccacggg ccaattgcaa tccccgccag caaatatccc aacacagcgc
     9361 caatccccag ccgcgaagag aaacagcact cctgcgagta aaaaatcgga accttccatc
     9421 agcggcctcc tggagacagc ggatttgcca accagtcacc gtaggctctg gcgtggctcg
     9481 ccagctcctt tgcgctttgc cgtctcgccc agtaaataat gatgggactt aaccaatgca
     9541 tccggcacat gcccgccgcc agttcaaagg ggcgcagcac atcgctcatc gggtagcgat
     9601 tcagcgcgtc ataacggtaa gcactttccg gctcgccggt ggtaatcacg ttacgccagt
     9661 actttcccgc cagttggttt cctcccggcc cgctggcaaa accacgactt aatacccggt
     9721 ccagccactc tttcagtagc gccgggcagc tataggtata aagaggatgc tgaaagacaa
     9781 tcacctcgtg ctcgcgcagt aatgcctgct cacgggggat atcaataaaa aaatcgggat
     9841 agtgcgcgta aaggtcgtgc acggtaacat tgctgagctg cgtggccggt ttaagcagta
     9901 cccggtttgc caccgagtcc tgagattccg gatgggcata cagcagcaaa actttcgctg
     9961 gctgagacat catccccctc ccggtatggt ttttgtgtat agtcgctgtt ttgggctacc
    10021 attgcgcccg gtgcggcagc tcgcccatac attacattat cataatgata agttaacata
    10081 gtctgaacat acggcgcctt atgattgttt tctcctcgtt acaaattcgt cgcggcgtgc
    10141 gcgtcctgct ggataatgcc accgccacca tcaaccccgg gcagaaagtc ggcctggtgg
    10201 gtaaaaacgg ctgtggtaaa tctaccctgc tggcattgct gaaaaatgaa atcagcgccg
    10261 acggcggcag ctacaccttt ccgggaagct ggcaactggc gtgggtgaat caggaaacgc
    10321 cagcgttacc gcaagcggcg ctggaatatg tcattgacgg cgaccgtgaa tatcgtcaac
    10381 tggaagcgca gctgcacgac gccaacgaac gtaacgacgg gcacgccatt gcgaccattc
    10441 atagcaagct ggatgctatt gacgcatgga gtattcgctc ccgtgccgcc agcctgctgc
    10501 acggcctcgg tttcagcaat gaacaactgg agcgcccggt aagtgatttc tccggtggct
    10561 ggcgtatgcg tcttaacctt gcccaggcgc tgatttgccg ttcagacttg ctgctgctcg
    10621 acgaaccgac taaccacctc gatctcgatg ccgttatctg gctggaaaaa tggttgaaga
    10681 gctatcaggg cacgctgatc ctgatctctc acgaccgcga cttcctcgat ccgatcgttg
    10741 ataaaattat tcatatcgaa caacaaagca tgttcgagta caccggcaac tacagttcgt
    10801 ttgaagtaca gcgcgccacc cgtctggcgc agcaacaagc gatgtacgaa agccagcagg
    10861 aacgcgtggc acatctgcaa agttatatcg atcgtttccg tgccaaagcc accaaagcga
    10921 agcaggccca gagccgcatt aagatgctgg aacgtatgga gctgattgcc cccgcacacg
    10981 tcgacaaccc gttccgcttt agcttccgcg cgccggaaag cctgccaaat ccgttactga
    11041 agatggaaaa ggtcagcgca ggctatggcg atcgcattat tctcgactcg attaaattga
    11101 atctggtccc cggctcgcgc attggtctgt taggccgcaa cggcgcaggt aaatcgacat
    11161 taatcaaact gttagccggt gaacttgcgc cagtcagcgg tgaaattggt ctggcgaaag
    11221 ggatcaagct cggctacttc gcccagcatc aacttgaata cctgcgcgcc gacgaatcac
    11281 ctattcaaca tctggcacgt ttagcgccgc aggagctgga acaaaaactg cgtgactacc
    11341 tcggcggctt tggtttcctg ggcgataaag taaccgaaga aacgcgccgc ttctccggtg
    11401 gggaaaaagc ccgcctggtg ctggcattaa ttgtctggca gcgtccgaat ctgctgctgc
    11461 tcgacgaacc gactaaccac cttgacctcg acatgcgtca ggcactcacc gaagcattaa
    11521 tcgagtttga aggcgcgctg gttgtcgttt cgcacgaccg tcatttgctg cgttccacca
    11581 ctgacgatct ctacctggtt cacgatcgta aagtcgaacc gttcgacggc gatctggaag
    11641 attatcaaca gtggttgagc gacgtacaaa agcaggaaaa ccagaccgac gaagcgccaa
    11701 aagagaacgc gaacagcgcc caggcacgta aagatcagaa gcgtcgggaa gcggagctgc
    11761 gtgcgcaaac ccagccgctg cgtaaagaga ttgcccgtct ggaaaaagag atggagaagc
    11821 tgaacgcgca actggcgcag gcggaagaga aactcggcga cagcgaactg tatgaccaga
    11881 gccgtaaagc ggagttgacc gcctgcctgc aacagcaagc cagcgccaaa tccggcctgg
    11941 aagagtgcga aatggcgtgg ctggaagccc aggagcagct tgagcagatg ctgctggaag
    12001 gccaaagcaa ctgatggcgc agataacgac gaccgatgcc aatgaattta gcagcagtgc
    12061 tgaattcacc cctatgcgcg gctttagcaa ttgtcatcta caaactatgc tgccgcgttt
    12121 gtttcgtcgt caggtgaaat tcaccccgta ctggcagcgg ctggagttgc ccgacggcga
    12181 ttttgtcgat ctcgcgtgga gtgaagaccc cgcacaggcg aaacataaac cgcgtttagt
    12241 ggtgtttcac gggctggaag gcagtctcaa tagcccttac gcccacggtc tggttgaggc
    12301 ggcgcaaaag cgcggctggc tgggcgtggt gatgcatttt cgcggatgca gcggtgaacc
    12361 aaaccgtatg caccgcattt accattcggg cgaaaccgaa gacgccagtt ggtttttacg
    12421 ctggctgcaa cgcgaatttg gtcatgcgcc aacggctgcc gtcggctatt cgctcggcgg
    12481 taatatgctg gcctgtttgc tggcaaagga aggcaatgat ctcccggttg atgcggcggt
    12541 gattgtctct gcgccgttta tgctggaagc ctgtagctat catatggaaa agggcttttc
    12601 ccgcgtttat cagcgttact tgctgaacct gttaaaagcc aatgccgcgc gcaagctggc
    12661 agcctacccc ggaacgctgc cgattaatct cgcgcagtta aaatcggtac gtcgcatccg
    12721 tgaatttgac gatctaatca ccgccagaat tcacggctac gccgacgcta tcgactatta
    12781 tcgtcagtgt agcgccatgc caatgctgaa ccggatcgcc aaaccgacgc tgattattca
    12841 cgccaaagac gatccgttta tggatcatca ggtgatccca aaaccggaaa gtctcccctc
    12901 gcaggtggag tatcaactga ctgaacatgg cggtcatgtt ggctttattg gcggtacatt
    12961 acttcatccg caaatgtggc tggagtcacg cattcctgac tggttaacaa cgtatctgga
    13021 ggcgaaatca tgttgattcc gtggcaagac ctctcccccg aaacgctgga aaatttaatt
    13081 gaaagctttg tgttacgtga aggcaccgat tatggtgaac atgagcgtac acttgaacag
    13141 aaagtcgccg acgtcaaacg ccagctacag tgcggagaag cggtgctggt atggtcggag
    13201 ctgcacgaaa cggtcaatat tatgccgcgc agtcagtttc gcgaataacc tgcaaccgtc
    13261 ggcggacgag ataatagtga tagctaagtc atggagttac tatgtctgcc aaacatccgg
    13321 tcattgcggt aacaggatcc agcggcgcgg ggaccaccac caccagcctc gcgtttcgta
    13381 aaatattcgc gcagttaaat ctgcatgcag ctgaggtgga aggcgacagt tttcaccgtt
    13441 acacccgccc ggaaatggac atggcgatcc gcaaagcgcg cgacgccggg cggcatatca
    13501 gctacttcgg ccccgaagcg aacgacttcg gcctgctgga acaaaccttc attgaatacg
    13561 gtcagagcgg caaagggaaa tctcgcaaat atctgcatac ctacgacgaa gccgtaccct
    13621 ggaatcaggt accgggaaca ttcaccccct ggcaaccttt accagaaccc actgatgtac
    13681 tgttttatga aggtttacac ggcggcgtag tcacgccaca gcataacgtt gcgcagcatg
    13741 tggacttact ggtcggcgta gtgcctatcg ttaaccttga gtggattcaa aaactgatcc
    13801 gcgacaccag cgagcgcggg cactcacgag aagcagtgat ggactcggta gtgcgttcaa
    13861 tggaagacta tatcaactac atcacaccgc agttttcccg cacccatctt aacttccagc
    13921 gcgttcccac cgtcgacact tcaaacccgt tcgcggcaaa aggtatcccg tcgctggatg
    13981 aaagctttgt ggtgatccat tttcgtaatc tggaagggat cgatttccct tggctgctgg
    14041 cgatgttgca aggctcattc atttcccaca tcaatacgtt agtggtacct ggcggcaaaa
    14101 tggggctggc aatggaatta attatgctgc cgctggtgca acgattgatg gaaggaaaga
    14161 aaatcgagta actttgctat tatgccggat aaaatactat ccggcttcac aacgggatag
    14221 ttaagtcacg cggcaaccac ttcatacgag tgagtaatat tcaccgcttt ttccagcatc
    14281 aacgccactg aacaatattt ctcggcagag agatcaaccg cacgcgcaac cgctgcgtct
    14341 ttcaggtcgc gaccggtgac gataaaatgc agattaatgt gcgtaaacag gcgtggtgcc
    14401 tcttcgcggc gttcagaggt caattttact tcacaatcga ccacatcctg acgccctttt
    14461 tgcaggatcg aaaccacatc gatggcactg caaccacccg ccgccatcaa caccatttcc
    14521 atcggacttg gtgctttatc gcctgagttg ccgtccatta aaatctggtg accagaggcg
    14581 gattcgccca gaaaagttaa cccttcgacc cacttcactc gcgcttgcat ttttgctact
    14641 ccactgcgtc aattttcctg acagagtacg cgtactaacc aaatcgcgca acggaaggcg
    14701 acctgggtca tgctgaagcg agacaccagg agacacaaag cgaaagctat gctaaaacag
    14761 tcaggatgct acagtaatac attgatgtac tgcatgtatg caaaggacgt cacattaccg
    14821 tgcagtacag ttgatagccc cttcccaggt agcgggaagc atatttcggc aatccagaga
    14881 cagcggcgtt atctggctct ggagaaagct tataacagag gataaccgcg catggtgctt
    14941 ggcaaaccgc aaacagaccc gactctcgaa tggttcttgt ctcattgcca cattcataag
    15001 tacccatcca agagcacgct tattcaccag ggtgaaaaag cggaaacgct gtactacatc
    15061 gttaaaggct ctgtggcagt gctgatcaaa gacgaagagg gtaaagaaat gatcctctcc
    15121 tatctgaatc agggtgattt tattggcgaa ctgggcctgt ttgaagaggg ccaggaacgt
    15181 agcgcatggg tacgtgcgaa aaccgcctgt gaagtggctg aaatttcgta caaaaaattt
    15241 cgccaattga ttcaggtaaa cccggacatt ctgatgcgtc tgtctgcaca gatggcgcgt
    15301 cgtctgcaag tcacttcaga gaaagtgggc aacctggcgt tcctcgacgt gacgggccgc
    15361 attgcacaga ctctgctgaa cctggcaaaa caaccagatg ctatgactca cccggacggt
    15421 atgcaaatca aaattacccg tcaggaaatc ggtcagattg tcggctgttc tcgtgaaacc
    15481 gtgggacgca ttctgaagat gctggaagat cagaacctga tctccgcaca cggtaaaacc
    15541 atcgtcgttt acggcactcg ttaatcccgt cggagtggcg cgttacctgg tagcgcgcca
    15601 ttttgtttcc cccgatgtgg cgcagactga tttatcaccc cgatatcaac tatgcacttc
    15661 gacaaacgct ggtgctatgt ttgcccgtgg ccgttgggtt aatgcttggc gaattacgat
    15721 tcggtctgct cttctccctc gttcctgcct gttgcaatat tgcgggcctt gatacgcctc
    15781 ataaacgttt tttcaaacac ttaatcattg gtgcgtcgct gtttgccacc tgtagcttac
    15841 tgacacagct gctactggta aaagatgttc ccctgccctt tttgctgacc ggattaacgc
    15901 tggtacttgg cgtcactgct gagctggggc cattacacgc aaaattgctt cctgcatcgc
    15961 tgctcgccgc catttttacc ctcagtctgg cgggatacat gccggtctgg gaaccattgc
    16021 tcatctatgc gttgggcact ctctggtacg gattgtttaa ctggttttgg ttctggatct
    16081 ggcgcgaaca accgctgcgc gagtcactaa gtctgctgta ccgtgaactg gcagattatt
    16141 gtgaagccaa atacagcctg cttacccagc acaccgatcc tgaaaaagcg ctgccaccgc
    16201 tgctggtgcg ccagcaaaaa gcggtcgatt taattactca gtgctatcag caaatgcata
    16261 tgctttccgc gcaaaacaat accgattaca agcggatgct gcgtattttc caggaggcgc
    16321 tggatttgca ggaacatatt tcggtcagtt tgcatcagcc ggaagaggtg caaaaactgg
    16381 tcgagcgtag ccatgcggaa gaagttatcc gctggaatgc gcaaaccgtc gccgctcgcc
    16441 tgcgcgtgct ggctgatgac attctttacc atcgcctgcc aacgcgtttt acgatggaaa
    16501 agcaaattgg cgcactggaa aaaatcgccc gccagcatcc ggataatccg gttgggcaat
    16561 tctgctactg gcatttcagc cgcatcgccc gcgtgctgcg cacccaaaaa ccgctctatg
    16621 cccgtgactt actggccgat aaacagcggc gaatgccgtt gcttccggca ctgaaaagtt
    16681 atctgtcact aaagtctccg gcgctacgca atgccggacg actcagtgtg atgttaagcg
    16741 ttgccagcct gatgggcacc gtgctgcatc tgccgaagtc gtactggatc ctgatgacgg
    16801 tattgctggt gacacaaaat ggctatggcg caacccgtct gaggattgtg aatcgctccg
    16861 tgggaaccgt ggtcgggtta atcattgcgg gcgtagcgct gcactttaaa attcccgaaa
    16921 gttacaccct gacgttgatg ctgattacca ccctcgccag ctacctgata ttgcgcaaaa
    16981 actacggctg ggcaacggtc ggttttacta ttaccgcagt gtataccctg caactgttgt
    17041 ggctgaacgg tgagcagtac atccttccac gtcttatcga taccattatt ggttgtttga
    17101 ttgctttcgg tggtaccgtc tggctgtggc cgcagtggca gagcgggtta ttgcgtaaaa
    17161 acgcccatga tgctttagaa gcctatcagg aagcgattcg cttgattctg agcgaggacc
    17221 cgcaaccaac gccactggcc tggcagagaa tgcgggtaaa tcaggcgcat aacactctgt
    17281 ataactcatt gaatcaggcg atgcaggaac cggcattcaa cagccattat ctggcagata
    17341 tgaaactgtg ggtaacgcac agccagttta ttgttgagca tattaatgcc atgaccacgc
    17401 tggcgcggga acaccgggca ttgccacctg aactggcaca agagtattta cagtcttgtg
    17461 aaatcgccat tcagcgttgt cagcagcgac tggagtatga cgaaccgggt agttctggcg
    17521 atgccaatat catggatgcg ccggagatgc agccgcacga aggcgcggca ggtacgctgg
    17581 agcagcattt acagcgggtt attggccatc tgaacaccat gcacaccatt tcgtcgatgg
    17641 catggcgtca gcgaccgcat cacgggattt ggctgagtcg caagttgcgg gattcgaagg
    17701 cgtaatacag gctaaaccat cggataaggc attcacgccg catccgacat attttgcctg
    17761 atacgtctta tttacgcccc aaccaccttc gccaccgcgt gggcgaaacg ttgcatccct
    17821 tcatcgatat ccgcatcttc caccaccagc gacggtgcta aacgcatcac atccggtccg
    17881 gcattcagca ccattacgcc aacctctgca cccgcataca ggaaatcacg cgcccgacct
    17941 ttgtagtgtg gtttcagctc cgcgccaatt aacagcccca taccgcgaat atcgctaaac
    18001 acatcgtact gctgatcgat cttctgcaaa tggtcaacaa aacgctggcg tttcgcctga
    18061 atgccttcca gcacttcagg ggtattgatg atatcaaacg ccgcgcccgc tactgcacag
    18121 gccagaggat taccgccgta ggtggaaccg tgagaaccag gatgaaacgc agaagcaatt
    18181 tccgccgtgg tcagcatggc gctaatcggg aagccgccgc ctaacgcttt cgcagaagtc
    18241 agaatatccg gcgtcacacc gtagtacctg taagcaaaca aatcgccagt ccgccccatc
    18301 ccgcactgca cttcatcaaa caccaataat gcctgatgtt gatcgcacag ctcgcgcaag
    18361 ccctgcaaaa actctggcgt cgctgccgtc acaccgccct cgccctggat cggctcaacc
    18421 accaccgcac aggtgtgatc atccatcacc gctttcactg catggagatc gttaaaggga
    18481 acgtggatga tgtctgccgg tttcggccca aagccgtcgg aatattttgg ctgcccaccc
    18541 accgaaacgg taaacagcga gcgaccatga aaagcgttat ggaaggcaat aattttggtt
    18601 ttgaacgggc tatgacgcac acaggcgtaa tggcgtgcca gtttaaaggc ggtttcgtta
    18661 gcttccgtgc cggagttcat aaacaccacg cgttcggcaa acgtcgcctc aatcagtttg
    18721 cgcccaagac gcagcgccgg ttcattggtg aaaacgttac tgatatgcca cagagtttcg
    18781 ccctgggttt ttaacgcgtt caccagcgca ggatggcaat ggcccaacgc cgtaactgca
    18841 atgccacccg cgaaatcgac atactccttg ccttgctgat cccagattcg gctgccctga
    18901 ccttttaccg gaataaactc tgccggagca taaatcggca ggatcacttc atcgaaagtc
    18961 gcgcgtgtaa ttgctgtttg ttcaattgcc atctcatgat caccctgtta cgcataaata
    19021 aatgtgaaat tataatcaca aaatatgcat aaaaaatcac tgaatggcaa tcggaaatca
    19081 gagatgcagg aaattagcca gcagttgatg tccttgttcg ctaagaatac tttctggatg
    19141 gaactgcaca ccttccagat cccactggcg atggcgaatc cccataatct cgcgggtttc
    19201 gctccaggcc gtcacgtcaa agcacgctgg taatgagtca ggttccacca ccagcgaatg
    19261 gtagcgtgtc acggtaagtg gatttgccag cccccgaaat acgccctcac cgctatgtgt
    19321 aatcggcgag gttttgccgt gcatgacctt tgcggcgcgc acaactttgc cgccaaatgc
    19381 ctgcgccatt gcctgatgac cgaggcagac gccaagaatc ggcaagcgcc cggcatagtg
    19441 gcgaataacg tcaagggaga tcccggcttc atctggcgta caggggccag gtgagatgac
    19501 aattttttgt ggtttaaggg cgtcgatatc cgccagcgtc aacgcatcgt tgcgcttaac
    19561 cagcacatcc gcccccagtt cacaaaagta ctggtagagg ttccaggtaa aagaatcgta
    19621 gttatctata agcaggatca tggcggctcc ggtacgaaag aaccgcgcta ttttactcag
    19681 attccccggc ttcgcttacc actttgctaa atatcatctg cagtgcggtg agatccccca
    19741 ttgccccact ctgatttgcc tgattccagg cctctttttc gataccctgc cagctcagtt
    19801 gatatccggc atgaatcgcc agttgctcga agaagatccg ctgtgccaga ccacttccca
    19861 cccggaaggg atgcagcacg ttgatttcac aatagtaatg cgccagccgc tcgacgaact
    19921 tcgctttctc caggccaacc agataacctt cttcctccag atcctgcatc agggcattgc
    19981 cctctttttc gatataagca aagtggcaga acggcgtatc gccctgataa atatcaactt
    20041 cacgcagttg ccctgcccag tcgaaaatat cctggtacag ctggcgatgg atagtgcgca
    20101 aatgcggtaa accgcgcacc agcggaccaa gctcaatggt cgcagcacgc agcgccgtca
    20161 tttcgtaagc ggcctgttcc agccgctgct gctggcggat gttcagccgg ttacgcatga
    20221 tatcaaggcc aggataaaga tacagatcgc gcccttcgcc gaatttatcg ctcatagtgc
    20281 ctcctcagct cttcaaggcg cgctaaagcc tctgcggcag taagagtgac taaaggcatc
    20341 tcgacgcctt caagacggcg actggcctgg aaattacgat tacgctgaag ctcccagaga
    20401 cgggactttt gcttatcggt gagtttcttc acttgatgcc tccctgaacg tgtccatttg
    20461 ccacaagtat aagcggcaaa tccgggttac gccgggagag cagaagcgcg ggcaagattg
    20521 cccgcgcgag aaatcattac ggcaggactt tagcggaaag gataactacc ggttttgacg
    20581 gcacattctg gtacggacca acatcatgag tcggcacctg ggaaatctta tcggcaacgt
    20641 ccatgccttt caccacttta ccaaataccg cgtaaccgaa atcacgctga ccatggtcaa
    20701 ggaaggcgtt atcggcaacg ttgataaaga actggctggt ggcgctgtct ttgtcagcgg
    20761 tacgcgccat cgcgatggtg ccacgcgtgt tgcgcaggcc gttatcggct tcatttttga
    20821 ttggcgggtt tggttttttc tgctgcatct gctcggtgaa accgccgccc tgaatcataa
    20881 agccaggaat gacgcggtga aaggtagtgt tgttataaaa accgctgttc acataatcga
    20941 caaagttttg cacagacact ggcgcttttt gtttatccag ctccagttcg atgttaccag
    21001 ctgaggttgt caacaatacg tgcgggtccc ctttcgctgc cattgctgcg ggagaaagag
    21061 cagaaagagc agaaagagcg aaaacagcag ccatcgccgc cagggtcgat ttgaacatga
    21121 gatttcctta acaaacagag cagaaaaaaa gcaagtgaag agattctaaa tagccttcaa
    21181 gaacaaggcc atccctttac ctaattttac gtatctgaaa tatatgtaac ctgacgatca
    21241 ctttttattc cgtgatcaaa atcacctctt aaaatgcaat ttagcaaccg attgcaataa
    21301 aacatttaaa cagatcataa aatcacctaa aatcgcccgc tcgcggcata tcccgctatg
    21361 ccattttatt tttgaacgca ggccaatcat gactaacagc aatcgcatca agctcacatg
    21421 gattagcttt ctctcctacg cactgactgg tgcgttggtt attgtcaccg ggatggtgat
    21481 gggaaatatc gccgattatt tcaatctgcc tgtttccagt atgagtaaca ccttcacctt
    21541 cctcaacgcc ggcattttaa tctctatctt cctcaacgcc tggctgatgg aaatcgtccc
    21601 gttgaaaacg cagttacgtt ttggctttct cctgatggtg ctggcggttg ccggtttgat
    21661 gttcagccac agcctggcgc tgttctcgac ggcgatgttc attctcgggg tggtcagcgg
    21721 cattactatg tcgattggta cattcctgat aacacaaatg tatgaagggc gtcagcgcgg
    21781 ttcccgcctg ttatttactg actccttctt cagtatggct gggatgattt tcccaatgat
    21841 cgccgcgttt ctactggcgc gcagcattga gtggtactgg gtttatgcct gcatcgggct
    21901 ggtgtatgtc gctattttta ttctgacctt cggctgtgag ttcccggcac tgggcaaacg
    21961 tgcaccaaaa acggatgctc cggtagagaa gaaaagtggg ggatcggcgt actgtttctc
    22021 tctgttgcgg cgctgtgcta catcctcggt cagttaggtt ttatctcctg ggtgcctgag
    22081 tatgccaaag gcctgggcat gagcctgaac gacgcgggca cgctggtgag taacttctgg
    22141 atgtcataca tggtcggcat gtgggcgttc agctttattc ttcgcttctt tgatttgcaa
    22201 cgcattctga ccgtactggc tggtctggct gcgattctga tgtacgtctt taacaccgga
    22261 acaccagtac atatggcgtg gtcaattctc gctctgggct tcttctccag cgcgatctat
    22321 accaccatca tcaccctggg ttcacagcag accaaagtac cgtcgccaaa actggttaac
    22381 tttgtcctga cctgcggaac catcggtact atgttgacct ttgtggttac cggcacgatt
    22441 gttgaacata gcggtccgca ggcggcactg cttacagcaa acggtctgta cgctgtcgtc
    22501 tttgtgatgt gcttcctgtt aggtttcgtc agccgtcacc gtcagcataa caccctgacc
    22561 tctcattaat tgctaatgcc ggacggcact atcgtcgtcc ggccttttcc tctcttcccc
    22621 cgctacgtgc atctatttct ataaacccgt tcattttgtc cattttttgc acaaacatga
    22681 aatgtcagac aattccgtga cttaagaaaa tttatacaaa tcagcaatat accccttaag
    22741 gagtatataa aggtgaattt gatttacatc aataagcggg gttgctgaat cgttaaggta
    22801 ggcggtaata gaaaagaaat cgaggcaaaa atgagcaaag tcagactcgc aattatcggt
    22861 aacggtatgg tcggccatcg ctttatcgaa gatcttcttg ataaatctga tgcggccaac
    22921 tttgatatta ccgttttctg tgaagaaccg cgcatcgctt atgaccgcgt acacctctca
    22981 tcttacttct ctcaccacac cgccgaagag ctgtcgctgg tgcgcgaagg cttctacgag
    23041 aaacacggca tcaaagttct ggtcggcgaa cgcgctatca ccatcaaccg tcaggagaag
    23101 gtgattcact ccagcgccgg acgtaccgtt ttttatgaca agctgatcat ggcaaccggt
    23161 tcctacccgt ggatcccacc aatcaaaggt tctgatactc aggactgctt tgtctatcgc
    23221 accattgaag acctcaacgc cattgaatcc tgcgcccgtc gcagcaaacg cggtgccgtt
    23281 gttggtggcg gcctgttagg tctggaagcc gctggcgcgc tgaaaaactt aggtattgaa
    23341 acccacgtta tcgaatttgc ccctatgctg atggcggaac agcttgatca gatgggtggc
    23401 gagcagctgc gtcgcaaaat cgaaagtatg ggcgtgcgcg ttcacaccag caaaaacacc
    23461 cttgagattg tgcaggaagg tgtcgaagcg cgtaaaacca tgcgttttgc cgacggcagc
    23521 gaactggaag tcgactttat cgtcttctct accggtatcc gtccgcgcga taagctggca
    23581 acccagtgtg gtctggacgt tgctccgcgt ggggggattg tcattaatga ttcctgccag
    23641 acttccgatc cggatatcta cgccatcggt gaatgcgcaa gctggaacaa ccgtgtattt
    23701 ggtctggtag caccaggcta caaaatggcg caggtcgccg ttgaccatat tctgggtagc
    23761 gaaaacgcct ttgaaggtgc tgaccttagc gccaagctga aactgctggg cgtagacgta
    23821 ggtggtattg gtgatgcgca cggtcgcacg cctggcgcac gtagctatgt ttacctcgac
    23881 gaaagtaaag agatctacaa acgcctgatt gtcagcgaag acaacaaaac cctgctcggt
    23941 gcggtactgg tgggcgatac cagcgactac ggcaacctgc tgcaactggt gctgaacgct
    24001 atcgagctgc cggaaaaccc ggattctctg attctgccgg cgcactcggg tagcggtaag
    24061 ccgtctatcg gtgttgataa actgccggac agcgcgcaaa tctgctcctg cttcgacgtc
    24121 actaaaggtg atctgattgc tgccatcaac aaaggctgcc acacagttgc ggcgctgaaa
    24181 gctgaaacca aagcgggtac tggctgcggt ggctgtatcc cgctggtcac tcaggtactg
    24241 aacgcggaac tggcgaaaca gggcatcgaa gttaacaaca acctgtgcga acactttgct
    24301 tattcgcgtc aggaactgtt ccatttgatc cgcgttgaag gcattaaaac cttcgaagaa
    24361 ctgctggcga aacacggcaa aggctacggt tgtgaagttt gtaaaccaac cgtcggttcg
    24421 ctgctggcct cctgctggaa cgaatacatt ctgaagccgg aacatactcc gctgcaggat
    24481 tctaacgaca acttcctcgc taacatccag aaagacggca cctactcggt gatcccgcgt
    24541 tctccgggcg gtgaaatcac cccggaaggg ctgatggcag tgggtcgtat cgcgcgtgaa
    24601 tttaatctct acaccaaaat caccggctcc cagcgcctgg cgatgtttgg cgcacagaaa
    24661 gacgatctgc cggaaatctg gcgtcagctg attgaagctg gcttcgaaac cggtcatgcc
    24721 tatgcgaaag cactgcgtat ggcgaaaacc tgcgtgggta gcacctggtg ccgctacggt
    24781 gttggcgata gcgtcggcct cggcgtggaa ctggaaaacc gctacaaagg catccgtacg
    24841 ccgcacaaaa tgaagttcgg tgtctccggc tgtacccgtg aatgttcaga agctcagggt
    24901 aaagacgtgg gtattatcgc cactgaaaaa ggctggaacc tgtatgtttg cggtaacggc
    24961 ggcatgaaac cgcgtcatgc ggatctgctg gcagcggata tcgatcgcga aacgctgatc
    25021 aaatatctcg accgcttcat gatgttctac atccgtaccg ccgacaaact gacgcgtacc
    25081 gcgccgtggt tagaaaacct cgaaggcggc atcgattacc tgaaagcggt gatcattgac
    25141 gacaaactgg ggctgaacgc tcatctggaa gaagagatgg cgcgcctgcg tgaagcggta
    25201 gtgtgtgagt ggactgaaac ggtcaataca ccgtccgcgc agactcgctt caaacacttc
    25261 atcaacagcg acaagcgtga cccgaacgtg cagatggtgc cagagcgcga acagcaccgt
    25321 ccggcaacgc cgtatgaacg tatcccggta actctggtgg aggacaacgc atgagccagt
    25381 ggaaagacat ctgcaaaatc gatgacatcc tgcctgaaac cggcgtctgc gcactgttag
    25441 gtgacaagca ggtagcgatt ttccgcccgt atcacagcga ccaggtgttt gcgatcagca
    25501 acattgatcc gttcttcgag tccagcgtgc tatcacgcgg gctgattgcg gaacaccagg
    25561 gcgagctgtg ggtcgccagc ccgctgaaaa aacagcgttt tcgcttaagt gatggcttgt
    25621 gcatggaaga cgaacagttt tccgtcaaac attacgaagc acgagtgaaa gacggcgtgg
    25681 tgcagctgcg cggttaatgt tttaacggga ggcgcaatgc ctcccctttt tgcatggtcc
    25741 tgtaatgacc ttcggtatat tgcaggacat tttttaaact ttttgtttta ttttttgttt
    25801 ttatttttta aaggataatc aaatgtttac agacactatt aataagtgtg cggctaatgc
    25861 tgcgcgcatt gcacgcctgt cggcaaataa cccgctcggc ttttgggtca gctccgccat
    25921 ggcgggcgca tatgtgggtc ttgggatcat cctgattttc acgctcggta atttgctcga
    25981 cccgtccgta cgtccgctgg tgatgggcgc gacctttggt atcgccttaa cgctggtgat
    26041 tatcgccggt tctgaactgt tcaccggaca caccatgttc ctcacctttg gggtaaaagc
    26101 gggcagcatc agccacgggc aaatgtgggc aatcctgccg caaacctggc tgggtaacct
    26161 ggtcggttcc gtcttcgttg ccatgctcta tagctggggc ggcggtagcc tgctgccggt
    26221 agataccagc atcgttcact ccgtcgcgct ggctaaaacc actgcaccgg caatggtact
    26281 gttcttcaaa ggtgcattgt gtaactggct ggtttgcctg gcaatctgga tggcgctgcg
    26341 cactgaaggg gcggcgaaat ttatcgctat ctggtggtgt ctgctggcat ttatcgcgtc
    26401 cggctacgag cactctatcg ctaacatgac gctgttcgcg ctctcctggt tcggcaacca
    26461 cagcgaagcc tacacgctgg cgggtattgg tcataacctg ctgtgggtga cgctgggtaa
    26521 tactttatca ggtgccgtat tcatgggatt gggttattgg tatgctacgc cgaaagcgaa
    26581 tcgtccggtt gcggacaaat ttaatcaaac tgaaacagct gccggttaat tactaagggg
    26641 tttttacgtg gatcatttgc ctatattttg ccaattacgc gatcgcgact gtctgattgt
    26701 cggcggtggt gatgtcgcgg aacgcaaagc aaggttgctg ttagacgcag gcgctcgctt
    26761 aacggtgaat gcattagcgt ttattccaca gttcaccgca tgggcagatg caggcatgtt
    26821 aaccctcgtc gaagggccat ttgatgaaag ccttctcgac acctgctggc tggcgattgc
    26881 agcgacggat gatgacacgc ttaaccagcg cgtcagcgaa gccgctgaag ctcgtcgcat
    26941 cttctgtaac gtagttgatg cgccgaaagc cgccagcttt attatgccgt cgattattga
    27001 ccgctcaccg ctaatggtag cggtctcctc tggcggcacc tctccggttc tggcgcgcct
    27061 gttgcgcgaa aaactcgaat cactgctgcc gttgcatctg ggccaggtgg cgaaatatgc
    27121 cgggcaatta cgcgggcgag tgaaacaaca gttcgccacg atgagtgagc gtcgccgttt
    27181 ctgggagaaa ttgttcgtta acgaccgcct ggcgcagtcg ctggcaaaca acgatcagaa
    27241 agccattact gaaacgaccg aacagttaat caacgaaccg ctcgaccatc gcggtgaagt
    27301 ggtgctggtt ggtgcaggtc cgggcgatgc cgggctgctg acgctgaaag gactgcaaca
    27361 aattcagcag gcagatgtgg tggtctacga ccgtctggtt tctgacgata ttatgaatct
    27421 ggtacgccgc gatgctgatc gcgttttcgt cggcaaacgc gcgggatacc actgtgtacc
    27481 gcaggaagag attaaccaga tcctgctgcg ggaagcgcaa aaaggcaaac gcgtggtgcg
    27541 gctgaaaggt ggcgatccgt ttatttttgg ccgtggtggc gaagagctgg aaacactgtg
    27601 caacgcgggt attccgttct cggtggttcc gggtattacc gcagcttctg gttgctctgc
    27661 ctattcgggt attccactca cgcatcgcga ttatgcccag agcgtacgct taattaccgg
    27721 acacttaaaa accggtggcg agctggactg ggaaaacctg gcggcagaaa aacagacgct
    27781 ggtgttctat atggggttga atcaggccgc gactattcag caaaagctga ttgaacacgg
    27841 tatgcctggc gaaatgccgg tggcaattgt cgaaaacgga acggcagtca cgcagcgcgt
    27901 gattgacggt acgctcacgc aactcggtga acttgctcag caaatgaaca gtccatcgct
    27961 aattattatt ggtcgggttg ttggcctgcg cgataaactg aactggttct ccaatcatta
    28021 atttaacccg ggccagagaa ttctggcctt cttaatgact ctttttatat attcagcaaa
    28081 taaaacataa ccccttaata aatattatct gctaatgggt tatattcacc gttacagcga
    28141 tttacaaaat taagtcattc gctcactgac gccaattaaa tattcctgta tcgttcttaa
    28201 cgagcagtct gcttatattc atataatcaa tgaatattaa ttaatatata atacataggg
    28261 aatgtaaatg aacaaattta ttaaagttgc actggtaggt gcagtactgg ctacgttaac
    28321 tgcatgtact ggtcatattg aaaaccgtga taagaactgc tcttacgact acctgctgca
    28381 cccggcaatt tctatttcta aaatcattgg cggttgcggt cctactgcac agtaagtctc
    28441 tgacagaaat cggctaacac cggtttaatt gcccgacgcc ctctggattt tccgagggcg
    28501 tatttgtttc tatgtccttc ttgcccccgc ccgtaaaaat attttcactt taaattcaat
    28561 tttataacta catccattag ccacagcgcg atcctgtgcg aaattttgtg atcttcctcc
    28621 acatcacata acataatatg ttgttatatt catcatgcat tgtcatgtta ccttttaaat
    28681 ggctgcaaac tctcccctac aacgtattgg acaagaaaaa ggtatcgcta tgggaagcca
    28741 ggaactccaa cgcaagctcg gattttgggc cgttcttgca atcgccgtcg ggacaaccgt
    28801 cggctccggt atttttgtat ctgtgggtga agtggcaaaa gcagcgggca cgccgtggct
    28861 tacggtgctc gcgtttgtca ttggcgggtt aattgtgatc ccgcaaatgt gcgtctatgc
    28921 ggaactatcc accgcttatc cggaaaatgg cgcagattat gtttatctga aaaatgccgg
    28981 aagccgaccg ctggctttcc tctccggctg ggccagcttc tgggccaacg atgcgccgtc
    29041 attgtcgatt atggcgctgg cgattgtcag caatcttggc tttttaacgc ctatcgatcc
    29101 gttgctcggt aaatttatcg ccgccggatt aattatcgcc tttatgttgc tacacctgcg
    29161 ctccgttgaa ggcggcgcag cgtttcagac gctaattacc atcgccaaaa ttatcccgtt
    29221 cactatcgtc attggccttg ggatcttctg gtttaaagcg gagaattttg ccgcccttac
    29281 caccactgcg attggcgcaa cgggcagctt tatggcgctg ctggcgggga tctctgccac
    29341 cagttggtcg tataccggca tggcctctat ctgttatatg accggcgaaa ttaaaaaccc
    29401 cggaaaaacc atgccacgag cgctgattgg ttcctgtctg ctggttctgg tgctctacac
    29461 cctgctggcg ctggtgattt ccggcctgat gcccttcgac aaactcgcca attctgaaac
    29521 gccgatttcc gacgccctga cctagatccc cgcactcggc agcaccgctg ggctctttgt
    29581 tgccatcacg gcgatgatcg tcattcttgg ttcgctttcc agctgcgtga tgtaccagcc
    29641 gcggctggaa tacgcgatgg cgaaagacaa cctgttcttt aaatgcttcg gccatgcgca
    29701 tccgaaatac aacacgccgg atgtctccat catcctgcaa ggggcgctgg ggatcttctt
    29761 catcttcgtt tccgatctca ccagcctgct gggttatttc accctggtga tgtgtttcaa
    29821 aaataccctc accttcggct ccatcatctg gtgtcgtaaa cgcgacgatt acaaaccgct
    29881 gtggcgtact ccggctttcg ggctgatgac caccctcgcc attgcgtcaa gcctcattct
    29941 ggtcgcctca acctttgtct gggcaccgat tcccggcctt atctgcgccg ccatcgttat
    30001 tgctactggt ctgcctgctt acgccttctg ggcgaagcgt agccgccagc tcaacgcttt
    30061 gtcgtaatgt tatctgaaga aaataaaatg ttggatattg ataaaagcac cgtggacttt
    30121 ctggtcaccg aaaatatggt tcaggaagtg gaaaaagttc tcagccatga cgttccgctg
    30181 gtgcacgcca tcgtggaaga gatggtgaag cgcgacattg atcgtattta tttcgttgcc
    30241 tgcggctcgc cactcaacgc ggcgcaaacg gcgaaacatc tggcggatcg cttttccgat
    30301 cttcaggtct acgccatttc cggctgggag ttctgcgata acaccccgta tcgcctcgac
    30361 gatcgttgcg cggtgattgg cgtttctgac tacggtaaaa cggaagaggt aatcaaagcg
    30421 ctggagctgg gccgggcctg cggagcactc actgcggcgt tcaccaaacg cgcggacagc
    30481 ccgattacct cggcggcgga atttagcatt gattatcagg ccgactgtat ctgggaaatt
    30541 cacctgctgc tctgctacag cgtggtgctg gagatgatca cccgcctcgc gccgaacgcg
    30601 gaaatcggca agatcaaaaa cgatctcaag cagttgccga atgcgctcgg tcatctggta
    30661 cgcacctggg aagaaaaagg ccgccagctt ggtgaactgg ccagccagtg gccgatgatt
    30721 tataccgttg ctgcgggtcc gctgcgtccg ctgggttaca aagaaggcat tgtaacgctg
    30781 atggaattta cctggacgca cggctgcgtg attgagagcg gagagttccg ccatggcccg
    30841 ctggagattg tcgaaccggg cgttccgttc ctgttcctgc tcggcaatga tgaaagtcgc
    30901 cacaccaccg aacgcgccat taactttgtt aaacagcgta ctgacaacgt gatcgtcatc
    30961 gattacgccg aaatttcgca agggctgcac ccgtggctgg caccgttcct gatgttcgtg
    31021 ccaatggagt ggctctgcta ctacctgtct atttacaaag atcacaaccc ggatgaacgc
    31081 cgctattacg gtggtctggt ggaatattaa tccctctccc ggcccggcaa cgggccggat
    31141 ttacgcaagg agttacccga tgaaaacagg tatgtttacc tgcggccacc agcggctgcc
    31201 tattgaacac gcatttcgtg atgcaagcga gctgggttac gacggcatcg aaatttgggg
    31261 cggtcgcccg cacgcgttcg cgccggactt aaaagcgggc ggcatcaaac aaatcaaggc
    31321 gctggcgcag acgtatcaga tgccgattat cggttacacg ccagaaacca acggctatcc
    31381 gtataacatg atgctgggcg atgaacatat gcgtcgtgaa agcctggaca tgatcaagct
    31441 ggcgatagat atggcaaaag agatgaacgc gggttatacg ctgatttctg cggcccacgc
    31501 gggctatctc acgccgccta acgttatctg ggggcggctg gcagagaact taagcgaact
    31561 gtgtgagtac gcggaaaaca tcggcatgga tctgatcctc gaaccgttaa cgccgtatga
    31621 atcgaacgtc gtgtgtaatg ctaatgatgt gcttcatgcg ctggcgctgg tgccttcgcc
    31681 gcgcctgttc agcatggtcg acatttgcgc gccgtatgtc caggcggaac cggtgatgag
    31741 ttatttcgac aaactgggcg ataaattacg tcatctgcat attgtcgaca gcgacggggc
    31801 cagcgacacg cattacattc ctggagaagg caaaatgccg ctgcgggaac tgatgcgcga
    31861 tattattgat cggggctatg agggttactc tacggtggag ctggtgacga tgtatatgaa
    31921 cgagcccaga ctctatgccc gccaggcgct ggaacgcttt cgcgcgctgc tgccggagga
    31981 tgagagatga aaaccctggc gacaatcggc gataactgcg tcgatatcta cccgcaactg
    32041 aataaagcgt tttctggcgg taatgcggtc aatgtggcgg tgtactgcac tcgctacggc
    32101 atacagccgg gatgcattac ctgggtgggt gacgatgact acggcacaaa gctgaagcag
    32161 gatctcgccc gcatgggcgt cgatatcagc catgtccata cgaaacacgg cgttaccgca
    32221 caaactcagg tggaactgca cgacaatgat cgcgtttttg gcgactacac cgaaggcgtg
    32281 atggccgact ttgccctgag tgaagaggat tacgcctggc tggcgcagta tgacattgtg
    32341 cacgcggcaa tctggggaca tgcggaagac gcattcccac agctgcacgc tgcgggcaaa
    32401 cttaccgctt tcgacttctc cgacaagtgg gacagcccgc tctggcagac actggtgccg
    32461 catctcgatt ttgcctttgc ctccgcaccg caagaagacg aagcgctgcg tctgaagatg
    32521 aaagcgattg ttgcccgtgg cgcaggaaca gtgattgtca cgctgggtga aaacggcagc
    32581 attgcctggg atggcgcgca gttctggcgt caggctcctg aaccggtgac ggttatcgac
    32641 accatgggtg ccggagattc gttcattgcc ggattccttt gcggctggtc tgcggggatg
    32701 acattaccgc aggcgatggc gcagggaacg gcgtgcgcgg cgaaaaccat tcagtaccac
    32761 ggtgcctggt aggtataacg ttggcgtgag catcttcacg ccaacgtgct gttacttgcc
    32821 ggaaaacgac cctataatcc gagtaattca ttctttattt cagggtcgat tatgtcagct
    32881 acggaccgct actctcatca actcctctac gctaccgtcc gccagcggct gctggatgat
    32941 atcgcgcagg gggtttacca ggccgggcaa cagatcccta ccgaaaacga gctttgtaca
    33001 caatataacg tcagccgcat taccattcgc aaagccatca gcgacttagt ggcagacggc
    33061 gtactgatcc gctggcaggg aaaaggcacc tttgtacaaa gccagaaagt tgaaaacgcc
    33121 ctgcttactg tcagtggttt taccgatttt ggcgtctcac aaggcaagtc gacgaaagag
    33181 aaagtgatcg aacaggaacg ggtcagcgcc gcgccgtttt gcgaaaagct gaacatcccc
    33241 ggaaacagcg aagtgttcca tctctgccgg gtgatgtatc tcgataaaga gccgctgttt
    33301 attgatagtt catggatccc gctgtcgcgt tatcctgact ttgatgagat ttacgtcgaa
    33361 ggaagctcca cctatcagtt atttcaggag cgttttgaca cgcgagtggt cagcgacaaa
    33421 aagaccatcg atatctttgc cgccacccgc ccgcaggcaa aatggctgaa atgcgaactg
    33481 ggcgaaccgt tgtttcgcat cagcaaaatc gcctttgatc agaatgacaa accggtgcac
    33541 gtctccgaac tcttctgccg cgccaatcgc atcaccttaa cgattgataa taaaagacat
    33601 taaccgtagg ccggataaga tgcgccagca tcgcatccgg cgatgctggc gcactgaatt
    33661 ttacatcccg cacgttcccc tcaccctaac cctctcccca aaggggcgag gggaccgttc
    33721 agtgcggaat ttagcttgat tgccgaacgg tgctatccag cattaaatac tcgcaatact
    33781 ctcacgcaaa atccgcagca ccgtctcttc accgctgcga ttcgggttaa tgcgaatcgc
    33841 acaatgttcg agttgtggat tcgcctggcg aaacgttcct gaaagtcgat agaagagcgg
    33901 cgggatttca tatttcgact ctgcaccgac cgggtaaggc aacgcgccac gcttttgcgc
    33961 ttcttccagc accctggcgg caatcagctg atgaaactcg acaatcaaca ccttcgactg
    34021 cgcattggca atgacggcgc ttttcacttc cgccaccgcg ccgccgttaa gcaaagccag
    34081 caaccgttcg gatactcccg cctgcaccgc atgcatcact ggcgcgaaca ccagaccacg
    34141 caagacttcc agcgcctgtg agccctggat ctggctaccg ccggagtaaa gcgtggcgcg
    34201 aatccgacta ataacatcgg catcgccaac cactgcacca acgccctccg gcccaaacag
    34261 cttgaagcag gagaatgtcg agacattcgc gccgcattca cagccgattc gcgctacctt
    34321 catcaccgca tagttatcat cggttaacgc tggaacacct gccgcgcgca acgttgccag
    34381 cacatctgcc agcacatagc tgtcctgcgg ctgctggcgc gtatgctgca ccagcgcggc
    34441 atcaggttgt tgctcgtcga cgacctgctt cagtgccgac aggtcattga aatcagcagt
    34501 aataagcgtc agccccatct gctcaataat aacccgtgtc gtcgggtaaa caggcgcgtc
    34561 atgcaccaga agacgctgcc ccggtttgag caaagccgcc agcccggcgc gaatcgcgcc
    34621 agtccccgcg ccctgcacca gcgccgcggc ctgtgcgtga aatgcatcag ccagcacctg
    34681 ctccacccgt tgggtaatac gcggttgatt cagacttgga gttaacccta aatcaccgcc
    34741 agcaagaaac tcgctgcctg gaaaatggcg gcaaatagtg tccaccagcg caaactgctt
    34801 ttgctgcgct tcagcaagag tcaggctttg caaaggaaac gtcttcatag gtgccttcct
    34861 taagccggaa cgaacaggcc aagccagtaa agaacattca gcagaatacc ggtgatcatt
    34921 actgccacga ccggtgccgc cattttctgt accggacggc ccagggattc gttgaggaag
    34981 taaatggcaa ccgcgataga gaatccggta taacccgcca tcttaattgc tgcgaaaatc
    35041 gaaccgacca gcagcgccac ttccatcagc atattcatgg cgttacggat gttatccgac
    35101 gcattacgca ccgacgggta gcgtcccagc catttgccga tcgaacgaag cagcaagact
    35161 tccgccgaaa taaccactgc gcctaatacc gctgcaacca tcggattcgg cgagagatag
    35221 cccaccgcat aaacaaaggt aaagcccgca actgcataca cacccgttgc taaggcggtg
    35281 gtggcaatca acggcacaaa ccccagtccg cgcataaatt ctgccagagc cgcctgatta
    35341 atcagcgttt gcgattgttc cggcgtcacg cctgcggaat acgctttctc cagtgtgaag
    35401 atcgaaactt cgctaccagc aaaaatcttc atactggcaa cggcggcaat caatgctccc
    35461 acgatggcga tataaggtaa gtttttgata atccgcgacg tgcgctcttc aaacaccgaa
    35521 agcccgctgg catcgatgtc attttcatca cgatgacgca ggtcgtgagt tatcgcgatc
    35581 cccagcagca tcaccatgcc aataaagatt tcgatggatt cagggttaag atgtgggaaa
    35641 tagcgcacga ctaccacacg ggtcatcaga acaaccacgg cggcgaccag actttgcttc
    35701 cagccaaact ggtagaaaat cgccaccagc gggaacagtg caaaagcaga aaccaccggc
    35761 gagcttaatt ccccgaggct acctaataca tccaccggca gcgcggtcag cagctggtta
    35821 actggcaaca ggcaagtaag gatcaacacg ccccagatag cgccaagacc aaacgccatc
    35881 aggctgttta tcgccagtac gccgaggata tcggtaggaa gaaagagtaa ccatgcgttg
    35941 agcagcccgg ttttcagggt aaaagagatc cccaccgagg ccacaaaacc aatgctcaga
    36001 ccaaaagcga tactccccgc ctcgcgacgg ttcatatagc cttcaatcag ttgcggcagg
    36061 atcgggtgga tgccgtcatg aaaaacagcc gccgagcgat gctccagaag cgatgtcata
    36121 cccgtcaggc acgccaccac gataatctga atatacagat ccatagtcgt gccttttatt
    36181 tcagatggtt aatcaacatc ggaatcgcgt gttcgacgtg ctcaacggaa aggccaaacg
    36241 ccactttccc ttcagcgatc attttggcga tatgctcgtc tttcgcttta atgcctggtt
    36301 tggcaatggt gcaacttttg ttatagccaa tcaccgcgat ggcaatcgac aatgccgcgc
    36361 ccgcaccggt attacacgcg ccaatgtaat aatctagttg cccggatttc accttcattg
    36421 ccgcttccat gtcgttgtga atgaaaactt caaaacagcc aggagccgtc gcttcaatgg
    36481 tttttttaat ctgctcacgc tgtaagcctg caacgccaat ctttttcatg agtctgtcct
    36541 tattggaaaa attgagaggg attttcacgt aacatcacat ccacatcggc ctgactgaat
    36601 cctgactggc gcaattgcgg aataaaggtg gtcaataaat agtcatagcc ataaccacca
    36661 ttggctttta aatgggagcg gcgcgtaata tccatcgaca gcatgacgcg gttcagcaac
    36721 ccacggtcac gtagcgcgtg gagcatcgca atgcgctttt cgtccgggta gtaactgttc
    36781 ttaccaatgg tgtcgaactg cacgtacgcg ccgagatcga tcatcttcaa aatgttgtcg
    36841 aggttgtctt tcagatcgca gtgaccaacg gtgacgcgcg aaagatcaac tccgtgggct
    36901 tgcaacagag ccagttgttc cagccccatg gtgctgaacg acgtatgcgt ggagatcggg
    36961 cgtccggtct ggttatgcgc cagcgcagcg gcaataaata ccttctcttc cagcggcgta
    37021 atctttcctt cgctggagcc gatctccgcg atgatcccgg ctttcagctc cgtgccatcg
    37081 ataccctgtt caatttcatc gaccatctcc tgcgccagtt cctgcacgct gcgggtcacc
    37141 acatgttccg ggaaaaacgc atcctggtaa taaccagtac aggccaccac gttgatcccg
    37201 gtttcgcgca ttacatcgag cataaattgc gcattgcgcc ccatgtaacg gttggtcatc
    37261 tcaatcacat tacgtacacc ccgggtcatc aggtcgttca tctcctggca aatgaacgca
    37321 tactgatcaa ggcggcagtc cacgttgttt ttaaagccgg agagatcaat atgcagatgc
    37381 tcatgggcca gggtgtaacc cgtcggatca aaactcatag ggtgtctcct gcaaaccgga
    37441 gggaggaaag aaaagagcga ccattttgtg gcggtggcgc acggaaaaat tcacacacgg
    37501 tagctcccac atcagaaagc gtggtgcgca caccgagctg cgtggcgacc agcccttgct
    37561 gataaaccag cactggcacc acttcacggg tatggtggct gtgaccaatg gtcggatcgt
    37621 taccgtgatc cgccatcacg accaggcaat catctggctg catcgcctca acaagccggg
    37681 caaggttacg gtcaacgacc tgcaaacgtt cggcataacg tgcgacgtct tctgcatgac
    37741 cagcgaggtc ggtttcctgg atattgatgc aaataaacgc cgtcggatgg gtgttaaatt
    37801 cgtcgagggt gatatccata atccgctggc tatccaccag gttttgccag ctcacaccat
    37861 aaggattgct gacgatatct gccaccttac ccaccagcac ggttggcacg cccgcttcat
    37921 acagtttttg cggcacctgc actttttcat caacaccgta gcccatatgc acgacctgga
    37981 agccgttgtc ataagcgcca gaacgcggcg cattaatacc aataaagcgc ccttctttgc
    38041 tttctgcggc atcgagaata cgctgactgt tggttaacag gccgccaaat gtaatgaccc
    38101 gaccgacctg tacctggtca cgcacgatac gaccaatttt gattgcgtcg tcaaaagaga
    38161 tcacagagag attggcggta atgttataga cttggcctaa atccgcctcg agattatcgc
    38221 caatcgcaac cgcctgattg accgacagaa attgcagatc atcgccacgg cgctccacct
    38281 gccagccagc ggaaactaat gcctgctcaa cacggtcaat cacatcgcga aaaggcatcc
    38341 gcagcggcgg taacgggcgc gtgcctaaaa tttcctgatg ccccataaag gtatcgccac
    38401 cttcatgttg cagctctgcc acgccccagg ttgcggaatc tgacggctgc atatcgcctg
    38461 gcgcataacc caatgtgttg attagcccca gcttctccag cgttggtagc tgcaaatgcg
    38521 gcaactggct caggatgtga ccacaggtat tcgccccggc atcttgcggg cgcaccagcg
    38581 tgacatcttt cattgcccct acgccaaagc tatcaatcac taacaccaca aatcgcgcca
    38641 tttatgcctc cagagggttg ccgaggctgt cataacgacc aactatttct ggttcgccgc
    38701 gctgaatacc ggacaccagc accacatcgc tacgggtgac aaaaatctgc gtgcgaaaac
    38761 agagcaccac tgcactgctc accggatact cgcctgccag cggcagggtg tagtcgatac
    38821 tgctgtcatc cacagttttg agattggttt cagtaatctt ttgattttct ggcgtaaaca
    38881 ccagcgcatg ttgcgcatga ccacgacggt agtaaccgcc gccgtagcag tagctgtcgc
    38941 cacggaaatg atgggagatt tcacttaacc agagcatcgc gatacgttca ggctgatcgc
    39001 cctgctggtt tgccggaata gtaccggtca gcgcatgacc gggttcggca tgagtcgcac
    39061 cgtattccgc cagcaatggc agcgaagtgc agctggtcgc tgaaggtacg ttcagttgct
    39121 caatcgcaat accagatttc gccagttgat cccgtgcttg taccagcgtg tgaagattcg
    39181 gtgttggcaa aactttccca gcagcctcat cccaaagcag gcaagggaaa tgggttagtc
    39241 cggctaaatg cagccccggc agtttcttaa tttcagcaat cacctcgtgc agcgaatgct
    39301 gtacaaaacc gctctcctgc cccggataaa gaaaatcatc gtcgctataa actttgagca
    39361 gcacaggctg gacccgtccg gccttaaccg ctgccgcaga aacttcccgc gctttgtcga
    39421 gagtaaacac ggtgatgatg tcggtgccct gttcaacggc gtcagcaacc tgatgacaag
    39481 ggatttgtac cagatgcccc tgatgcgcca ctggcaaacc agcgcggcgc atgactcgcg
    39541 cctctttgta atccaccgcc acaatgccgc tgtagcctaa tgccagcaat ttttccgcca
    39601 gccacggatt acgaccaaat tgcttggtca tcagatacag ttcaatgccg taaagccgcg
    39661 ccgtctcaat gagccgctta ccgttttcca gtatctgatc cacgtcgatc acccagctgt
    39721 ccggggcgat tttgccctgc tgccacaaac ttagtgcggc ggaaatcagc gccgggttct
    39781 ggcgtttcaa tgcttctaca aacatcttgt tgcctcgttt ctgttccttg aattaaatat
    39841 tcattttttt gaatatttag gttgtgcgac gtggatttgt cgggagcttc aaacctcttc
    39901 gttagcagcc atccataatc cataaagatt ggccagaaaa tagccttctt cacacgggtt
    39961 aacttccaga gcgaattcct tcaacaactc ctgatgcaat tgcacaacgg cttgccagtg
    40021 gctggattgc gccagttccg ccagcaactc gttatccagc ggctctattt cttcaccacg
    40081 gcgactgcgc atcagtgcac tcgccatatg tgtcatcgcc atcgttcctt gctcactgcg
    40141 caccggcaga tggcactctt tttccagtac gttgacgacc tgcatcatgc ctttgcagac
    40201 gtccttatca ataacgcctg cctcgcaaag caggttgagt ctggtttcca tatcagatgc
    40261 ctttcgttta ataactgggt tcctgttccc cactcaccac ccgttgttga tgggcaagca
    40321 gggcgtgttt atctacaaca gcacgcagca attgttgcat tgggtaatca tcgactcgcg
    40381 tcaggaccac ggcttcggtg gcctgtaaaa atcgcggatc gtctgttagc ggcgtcgcct
    40441 ctaatcccag catggtcagt tcgttttccg ccaccacgtt ccagatcacc gcatcgacat
    40501 cgcctttaac aatgcgttgt aaactctcgt gataagagag atcgactcgt tccacatcac
    40561 tatcgccaaa aaagacatcg gtcatgattt tctgatccgc cgaacgacta tcgagaccta
    40621 ctcgcttaac atttccagac tcacctttgc ggcatatcag ttgatgttca ccaacatacg
    40681 tatgcgggcc aagctcgagg gcgatgcaca aattgttttg gctgagataa ctttccgccg
    40741 ccaggcggga aactactgcc atgtcataga caccgttaag taaacactcc acgcgaatat
    40801 ccgcgccgcg catatgcgca tagtaaaaag gaatgccatc aaactgggct ttcaatccgc
    40861 tcgctaaacc ttcgtacaag cgggtatagg gcaaaggcat cgcacatacc acgttgttga
    40921 tatccacatg actcagcaat gctttgttat ccatctcgac aagataactg ccattgcgcc
    40981 cacggcgttc aatccgtatc gctccgcttg attccagcgt tttcaacgcg gcctgcgtca
    41041 ggccaaccga tgaacggcat tcagttgcca gctcatctat ggttttcagt cgattaccgc
    41101 atttttcacc caacaaataa cgggccagtg tcgtgatgac gacgccttct tttttgataa
    41161 acgttcgacg cataatatat tttcaataaa atgaatattt atatcttcat taaaatgaag
    41221 ataagtggcg acgacgagat gtggaaacgg cgaggcgctt cacatttttt cgggttgttg
    41281 cgaaaagagg ataaaaaaac cgggtttccc cggtttcaga gtgatgacga agtgatttat
    41341 tcgacaatgc gagcaaaggg taattcccac tggcgcttgc ctgctcccct caccctaacc
    41401 ctctccccaa aggggcgagg ggaccgtccg agcgttttga tattacggct tcgccacaaa
    41461 accaatcgct tcgtacaccg ctttcagcgt acgggaagcg tgtacactgg ctttttctgc
    41521 gccgtctttc atcacctgtt gcaggaaggc ttcatcgttg cggaaacggt gatagcgttc
    41581 ctgcaattca gtcagcatac cggaaacggc atcagccact tcacctttca gatgaccata
    41641 catcttgcct tcgaactgtt tttccagttc tgggatgctc tggcccgtta ccgcagaaag
    41701 gatatccagc aggttggaaa cgcccgcttt gttctgcaca tcgtagcgaa ctaccggcgg
    41761 ctcgtcggag tcagtgaccg cgcgtttgat tttcttcact accgatttcg gatcttccag
    41821 caggccgata acgttattgc ggttatcgtc agacttggac atcttcttgg tcggctccag
    41881 cagcgacatt acgcgcgcgc cagatttcgg aataaacggc tccggcacct taaagatctc
    41941 gccatacagc gcgttgaaac gctgggcgat atcgcgactc agttccaggt gctgtttctg
    42001 gtcttcaccc accggtacca gattagtttg atacagcagg atgtccgcag ccatcagcac
    42061 cggatagcca aacagaccag cgttgatgtt ctcggcataa cgcgcagatt tatctttaaa
    42121 ctgcgtcatg cgactcagtt cgccgaagta ggtatagcag ttcagtgccc agcctaactg
    42181 cgcatgttct ggtacgtggg actgaacaaa aatggtgctt ttctcaggat cgataccaca
    42241 agccagatac aaggccagcg tatccaacgt cgctttacgc agcttctgtg catcctggcg
    42301 cacggtgatc gcgtgttggt caacgataca gtaaatgcaa tggtagtcat cctgcatgtt
    42361 tacccactga cgcagcgcac ccatgtagtt accaatggtc aattcacctg agggctgtgc
    42421 gccactaaaa acgatgggct tagtcatttt tcgattcctg attttcgcta tgcggaagcc
    42481 ctaatgcggg cagaaggtca tttatagact gataaattac atcaggctgg ctgagatcga
    42541 tagcctcgcc gtagttgtat ccgtaggtta agccaactga tgggcaacct gccgcttttg
    42601 ccgcctgaat atcattccgt gagtcgccga caaacagcat ctgttgtggg gcaattccca
    42661 tccgctcagc caccagtaac agcgggtccg gatgcggttt tttgttttgc acatcatcgc
    42721 cgccgataac cacgctaaag tatttggcaa tatctaaggc ttcgagcagc ggcgcgacga
    42781 acggcgtcgg tttgttggtg accaagccta gcggcaggcc tttagcctgc aacgcgccca
    42841 acgtatcggc aacgtgcggg aacaaaaacg tcccctcttc ggcaacctcg ccatagtagc
    42901 gatcgaacag tttacgcaga atacgtacct gttcttctgc cggaatgtcg tcatcaacgg
    42961 gcggtttccc cattgtttta cgcagagtcg cacgttcctg acgcgcccag gtcaatgcgc
    43021 gctccatcag aacatctgcg ccgttaccaa tccaggtaat aacgcgttct tcacctgcga
    43081 cgggcaactc cagcgcatac agcgccatat ctaccgcagc agcaagacca ggagcactgt
    43141 cgaccagcgt accatcaaga tcaaaagcga cgccgcgaat atcttcaaac ttattcatga
    43201 cttacctttg ccagttcact gcgcatttca tcaatgactt ttttatagtc tggctggtcg
    43261 aagattgccg aaccggcgac gaacatatcc gcgcccgccg cagcgatttc gccaatgttg
    43321 ttcaccttca cgccaccgtc cacttccaga cgaatgtcaa agccagactc gtcgatacgg
    43381 cgacgtactt cgcgcagttt atccagtgtt tgaggaatga aagactgacc gccgaaacca
    43441 gggttgacgg acatcagcag gatcacatcc agcttatcca tcacgtaatc cagatagctc
    43501 agaggtgtcg ccgggttaaa taccagaccc gctttacagc cattttcttt aatcagttgc
    43561 agcgtgcggt caacatgctc ggaggcttct ggatgaaagg taatgatgct ggcaccagcg
    43621 gcagcgaaat caggcacaat gcgatcgacg ggtttcacca tcagatgtac gtcgataggg
    43681 gcggtaatgc cgtagttacg caaggatttc agcaccattg gcccaatcgt cagattggga
    43741 acatagtggt tatccatgac gtcaaaatgc acgacatcag cgccagctgc cagggctttt
    43801 gcggtatctt cacccaggcg ggcaaaatca gccgacagaa ttgagggggc aatcaaatac
    43861 tgtttcatcc gcttctcctt gagaattatt ttttcgcggg tgaaacgact cctggtttgt
    43921 acaaagccag cagttcgtcc acctttttac gtgtgccgcc gttgctgctt atactgcgtc
    43981 gaactttgac gacatgcaat tttgcgcgct gataccactc acgcgttaac atcgtatcgt
    44041 gattggagat cagcactgga atatggcgct caaccagacc ttcggcgatc tccgccagat
    44101 gcgcttgttg ttcaagcgta aaactgtttg tgtgatacgc cgtaaagttg gcggtcgcag
    44161 acagcggtgc ataaggcgga tcgcaataga cgacggatgc atcatctgcg cgctccatgc
    44221 tatcggcgta agactcacaa tagaaaaagg cattctgcgc tttttcagcg aagtgataca
    44281 actctgcttc cgggaaatag ggttttttgt agcggccaaa cggcacgtta aactcaccgc
    44341 gcagattgta acgacacagg ccgttgtaac cgtagcggtt caaatataaa aacagtaccg
    44401 cccgacggaa cggatcctgg cttttgttga actcttcgcg gaactgatag taaacctcgg
    44461 cgcaatttgt ttcgggaaca aacagctcgc gtgcggcctg tacgtactca tcagtacgca
    44521 tcttcacaat gttatagaga ctgatcaggt cgctattgat atcggcaagg atataacgag
    44581 aaaagtcggt gttgagaaac accgacccgg cacctacaaa aggctcaacc agacattcgc
    44641 ccttgggcaa atgccgttta atatcatcaa gcaggggata cttgccccct gcccacttca
    44701 aaaaagcgcg atttttcttc atgccgatta actaattaca ccttctccgg ctgtggagaa
    44761 agctccgaca gcatccagcg cttcaggcag ctcccgcaac gataaaccat tgcgggagat
    44821 aaccttgatt acttcagatc ggcctgtacc tgacgcagcg gtttcgccca cgggtttttg
    44881 gcctgaacat ctgctggcaa tgtagatacc gcttttttcg cctcttcttt cgaagcgtac
    44941 acgccagaaa ccaggacata ccacggctga ccattacgcg tcgtttcata gacaacgtag
    45001 tttttcagat tctctttctt cgcccaaccg ttcaggttgt cgtagttaga ggaactgctc
    45061 agctgcagag tgtaatggct ggacggtgcc gatttcaacg aaccaacatt acctgcggtc
    45121 ttccctccag cggctggtgt tgccgtggtt tgcgccgggg atgccgtctg tactggagcc
    45181 gtagtcgccg tctcttttgg tgctggcgta gaagtcgcag ccggtgcctt cgtgctcgcc
    45241 acaggggcag ctggttcagg ttcagtacgc ttcggcgtct gtgctaccgg cttcggctcc
    45301 gttttcacgg ttgcttgcgg ttttttcggt tcaatcaccg cctgctgacg aactgggcgc
    45361 gtagtggacg gacgttcagc ggtttgcgtt ttcgccgtgt cacgcgatgc attgccattg
    45421 cgaacaggcg caaccgttgc gggttcagtc ggcaacgtgg aattgaccgc cacattgttc
    45481 agctgttgct gattttgtgg ctgggtcagc gcattgttca ggtcaccctg cacttcaaca
    45541 cgttgttgac catccgttgc caccggggtt tgcccttgag tcggcgtaga agagatcggt
    45601 ggcagagaaa catcctgctg agtattttcc gcagacgtgg ttcccggcgc tggctgcacg
    45661 ccattcgcct gatcggtcgc attgccagca agatcaatac tcttctcgcc agacgcggtt
    45721 tgatcgctgg aagtggtcga gggggctttt agcgcagaac cgataccgat gatcaacagc
    45781 agtagaacca gaatgccgac gcccatcatc atatactgac gagaagcggg tttactggct
    45841 gcttttttgc gcttacgcgg acgacgctct acgcgctctt catccacggt ttcatcttcg
    45901 gattcgtcaa tttcttcttc gatttccggt tcctcattgc gctctttttg cgcacgagtc
    45961 ggacggcgat cgtcagtgtc atcaagttca atatcatcaa aattgatctg cggttcgcca
    46021 cgttcagtac gctcagaacg ttcagaagat tgacgagaac gaccagtacg acgatcgctg
    46081 ggatcgggtt tcagctcgtc ttctggtttg aattcatcca tttaacaccc cactaaaagg
    46141 ttaatgctta ccacgttgca attaacctga agctaataga ccgcttgata agcggcctga
    46201 cctttcttgt tgttacgctg attgacaatc ggcaatggcg ttaagaacaa gctcgtgcga
    46261 aacgccgctg cgaacttcac tcttaccaat tgccaacgga agaattaagc gcatctctcc
    46321 cgcaaggact ttcttgtcac gcagcatatg cggtaaatac gcctgcgcgg acatttcgcg
    46381 cggcccattg accggtaacc cagcccgcgt gagcagggtt ataatacgct gcgtttcggc
    46441 agaactaaac tgcccgagac gttccgacgt ccgcgccgcc atcaccatac ccgcagcgac
    46501 cgcttcacca tgtaaccaat tgccataccc catttcagct tcaatggcat gaccaaaggt
    46561 gtgtcccaga ttcagtaaag cacgtaaccc ggtttcgcgc tcgtcggcgg cgacaacttc
    46621 tgccttcagt tcacaacaac ggcgaataca gtacgccatt gccggaccgt ccagacgcaa
    46681 caacgcatcc agattctctt ccagccagtt aaaaaacgca ccgtcaagaa taatgccgta
    46741 tttgatgact tctgccagcc ccgacgctaa ctcacgcggg ggaagcgttt tcagacagtc
    46801 gagatccacc accactgaag caggctggta gaacgcgcca atcatgtttt taccgagggg
    46861 atggttgacc gcagttttgc cgccaacgga ggaatcgacc tgcgacagta acgtcgtcgg
    46921 gacttgaatg aaacgaacac cgcgctgata actcgccgcc gcgaagccgg tcagatcgcc
    46981 cactacgccg ccgccaagcg ccaccagcgt agtatcgcga ccatgcggct tttgtaacaa
    47041 cgccgtaaag acggtatcga gtacagctag gcttttatac tgctcgccgt cagggaggat
    47101 aacgctatcg acgttaacac ccgcctgttc aagtacgccg cggaccttat cgagatacag
    47161 aggagccagg gtttcgctgg tgaccaacat gacctgctcg cccgatttca gcggtaagaa
    47221 tgaagctggt tcattaaaca aaccagatgc gatggtaatt gggtaactac gttccccgag
    47281 agtaacgaca atcctctcca taacgcgaca tccaccttaa ttactgtacc cgcagacgag
    47341 tgtatataaa gccagaatta gttgctttcc agcatgtgaa taatctggtt tgcaaccact
    47401 ttagcgcttt gatcatcagt acgaatggtc acgtcggcaa tctcttcata cagcggattg
    47461 cgttcattgg ccaacgcttc cagaacttca cgcggcggtg tttcaacgtg cagcaacggg
    47521 cgttttttat cacgctgcgt gcgtgcaagt tgcttttcga tggtcgtttc aagataaacg
    47581 acaacgccac gagcggaaag acggttacgc gtttcacggg atttcacaga gccgccgcca
    47641 gtagccagca caataccctg tttctcggtc aactcattga tgaccttttc ttcgcgatcg
    47701 cggaagcctt cttcgccttc taaatcgaaa acccagccca catcagctcc ggttcgtttc
    47761 tcaatctctt gatcggaatc gtaaaattcc atattgagtt gttgagctaa ctggcgccca
    47821 atagtgcttt ttccggcacc cataggccca accagaaaga tattgcgttt ctctgccatt
    47881 ttttcggtac tactaagact attcgttaat gataaacccg cttcgctcag agagcgccgc
    47941 aggacatgaa ctgaaacctc ataagatatt gcgagagtca gactgaaaat tatctcaata
    48001 ctccagcggg tttggcaact gaataaatca cccatccctc tgcatatctg gtcgctgcaa
    48061 gcgcgctgcc ttgctaccac cgctctggcg ataaatcacc gggtaagatt agcgtaaaaa
    48121 agacagcaaa atgccgcctg aatgataaat catcatcatg gaacgcaggc agcgaagagc
    48181 accaactctc aaatcggtac tccttgtatg ctaaatacct gcgcgcgtca aatagatgaa
    48241 acacgttcag cataaaaaca ttaccgcttt tacggctgtt tactcactgg aaaccagtcg
    48301 tggcgtgata aacaccacta actcgcgtcg ttcatcttct tttccgtcat gacgaaataa
    48361 ttgcccgaac cagggaatgt cgccaagcaa cggtacgcta tcctgacccg atttattttt
    48421 acgggtaaaa atgccgccca gcgccaacgt ttctccgctt tttacctcga cctgcgtttc
    48481 gatctcctgc ttatcaatcg ccagcacttc gccatcggcc tgctgtagca cctgccccgg
    48541 aacgttctgg ctgatgtgta atttcagccg gatgcgacct ttttgtaaca ccgtgggcat
    48601 gacctccatc cccaggacgg cctctttaaa ttccaccgac gtcgcgccac tttccccgct
    48661 ggaaacctga tatggaattt cgctctcctg tttaatgctg gcaggctgaa gatgtgaggc
    48721 cagcagacgc ggactggcga taatatccag ctgctgtttt tgttcgagcg cggaaagctc
    48781 aagatccagc aagcgtccgt tgatgcgccc aatgttaaaa ccgacatgcg ttgtcgccgt
    48841 cgctacggag aggtcgctac caagcgtggt gacttgccca acgccaccag cgtgttgcgc
    48901 atcggccagc gtccatttca cgcctaactc acgcaaactt ttttcattaa tggtgacaat
    48961 atgcgccgac agctcaacct gcccgaccgg cagatccatt tgcgctaccc actgttcaag
    49021 cgcgcttaac gccgttttgt tatctcgtag caaaaggcga ttggtgcgtt tatcgacggt
    49081 catactccct ttggcactca gtagcttctc ccccgctttc gccagttctc ccgcgtcggc
    49141 gtattgcagg gttatactgc gattttccag cggcagattt gcctgcgccc gcgcctgctc
    49201 cgcctcctgg cgggcgatat tgtcattctg ccaggcaatg gaatgcactg agagaatgtt
    49261 gccttcctgc cgcgttatca gtccggcgct tttcactaca gtttgtagtg cctgcttcca
    49321 gggaacatct gttagatgta acgacaccgt accgctgacg tctggcgaca cgaccaggtt
    49381 caacttctcc tgttcagcca gcgcctgcaa cacctgagct accggaacgt catccaccat
    49441 cagcgtcact ttttgcggct ttgccgcctg tacgccgggt atcagcatca acagtagtgc
    49501 ggctatccat tgcttcattt gtatctcctt gccgttgcca caaccattgt ggcggttcgc
    49561 agtttgtccc ggtacccagc gttagtacgt ctggcgtcag ctgtaaaatt gtccagccgt
    49621 tttccagcac atcgttttgc tgcacccgtc gccatttctt ttgcccgtct tttattacac
    49681 cgatgatgcg ctcgcctcgc cctaccatcc cctgatagcg ccactggcta agttcgctaa
    49741 tccggcatat agatcttccg gcgatttaaa agggtcacgc ataccggtta aaaggcacaa
    49801 tgcaatacct gccaacaacc agcgtttaac cctcatgcgg catctccagt tgtagcgtga
    49861 acaaaagatc atcaccttcc acgcttaacg aaaaacggct cacgctgacg ttgcgctctg
    49921 ccagccgtgt aaatgccgac ggcactgctt cccacagcgt tttcaacgcc aactcacctc
    49981 cctgcgcgga tggatgccag gaaaccagtt gcgcgccgga taactgaaaa tccagtggcg
    50041 aaaagggcag cgttttttcc tcgctgaagg gagtggtgtc taccaggcga tacagtgcgg
    50101 cccactgctg gtgatgactt gcccgtagtc gaattaatgc gtcgcgctct tcatggtgtg
    50161 tcgatgatag aaaaatgagc gtaactaaca tcagcaacca gaatgcccag caaaactggc
    50221 ggaggcgggg tgatgtggcg aaccaccagt caaagaacat gttcatcgct aaccttcctt
    50281 gttaactgat actcaaattg ccagcgtccc tgcgcatcct gctgcgtggc tccccgctga
    50341 ttgagatgaa aagacgcatc ctggcggagt gacgtttcta gtgcgtttaa cgcggtaatg
    50401 cttgttgtca gccccttgat ctccagcgtt ccctgctgcc agcttatcgt tgtcagccag
    50461 gcgtgctcgg gtaaaagcgc cgccagtgct tccagcgcag attgccagtc gcgggtaaat
    50521 tgtcgctggc gctgccgttg cgaacgctgt tcgcgtaact gctgttgctc cagcaaacgt
    50581 ggcttcgtta tctgtaagct gcgggcgagt tgttgttccg cctgaagcaa aactgcgtct
    50641 atgcgcgctt cggcgctgcc tgtcagacgc agtattagcg ttatcccgac ggccagcagc
    50701 agaggcgcaa cgaacatcag caaccagaaa cgcagaaaag cggtccggcg ttgctgtcgc
    50761 cagggcaaaa aattaattgg cggattcatc agtacgcctt cccaagcgcc agccccagcg
    50821 cgatggcaaa gtctccacca ggcggcggta gcggcggctg acgaacagaa acggcctccc
    50881 aggggtcaaa tccgccttcg ccacatatcg cgacgctgtc gggatcaacg gataacgctg
    50941 ccgccagctc cttcgcgcta gtcatcccca ccgccagttt gcgcccccag ctatagcgcg
    51001 tcgcccacag ccactgttcg ttatcacgcc aggccagaca ttgctgatga gaaggtaaaa
    51061 aaggcaggaa tcgctgtaat gcactggcat ccggggtgat cgcactaaca tgaacacgca
    51121 acctttctgc cagcgtaagc agcgttgcca gctctttgct ttgcgcggca gtcacgttat
    51181 aagcggggct gagtgagtct tcgctgtaat cgaagcgcag ggagtccgga tccatatcca
    51241 gctcgcgggc catcgtccct gacagccagg ccgtttgctc ccgctcacca agggacatcg
    51301 acgggcgcgg aaatgaccgc tgtaatgtgc gactggcggg aaacgccaac ataatgtgat
    51361 gacgctgcgg cagttcgcga ctccacggta acaacgtttt agccagctgc tgcgcatcaa
    51421 caatccgccc atctttgata atgtcgtgcg ccagcggcaa ccgccaccag cgttgcaaaa
    51481 agcattcttt tgcgccccgc acgatcgcaa ccgctgccgc ttcttgctgt tgtaaatgca
    51541 aaccaatttg ccagatctta aatgtcattg tgatgatctc cttatcaccc gtcactctga
    51601 cgggtatatc aatgcgtctg gcttgccttt atactaccgc gcgtttgttt ataaactgcc
    51661 caaatgaaac taaatgggaa atttccagtg aagttcgtaa agtatttttt gatccttgca
    51721 gtctgttgca ttctgctggg agcaggctcg atttatggcc tataccgcta catcgagcca
    51781 caactgccgg atgtggtgac attaaaagat gttcgcctgc aaattccgat gcagatttac
    51841 agcgccgatg gcgagctgat tgctcaatac ggtgagaaac gtcgtattcc ggttacgttg
    51901 gatcaaatcc caccggagat ggtgaaagcc tttatcgcga cggaagacag ccgcttctac
    51961 gagcatcacg gcgttgaccc ggtggggatc ttccgtgcgg caagtgtggc gctgttctcc
    52021 ggccacgcgt cacaaggggc gagtaccatt acccagcagc tggcgagaaa tttcttcctc
    52081 agtccagaac gcacgatgat gcgtaagatt aaggaagtct tcctcgcgat tcgcattgaa
    52141 cagttgttga cgaaagacga aatcctcgag ctttatctga acaagattta ccttggttac
    52201 cgcgcctatg gtgtcggtgc tgcggcacaa gtctatttcg gtaaaacggt cgaccaactg
    52261 acgctgaacg aaatggcggt gatagccggg ttgccgaaag cgccttccac ctttaatccg
    52321 ctctactcaa tggatcgtgc cgtcgcgcgg cgtaacgtcg tactgtcgcg gatgctggat
    52381 gaagggtata tcacccagca acagttcgat cagacacgca ctgaggcgat taacgctaat
    52441 tatcacgcgc cggagattgc tttctctgcg ccgtacctga gcgaaatggt gcgccaggag
    52501 atgtataacc gttatggcga aagtgcctat gaagacggtt atcgcattta caccaccatc
    52561 acccgcaaag tgcagcaggc cgcgcagcag gcggtacgta ataacgtgct ggactacgac
    52621 atgcgccacg gctatcgcgg cccggcaaat gtgctgtgga aagtgggcga gtcggcgtgg
    52681 gataacaaca agattaccga tacgctgaag gcgctgccga cctatggtcc gctgctgcct
    52741 gccgcagtca ccagcgccaa tcctcaggga gcgacggcga tgctggcgga cgggtcgacc
    52801 gtcgcattga gtatggaagg cgttcgctgg gcgcgtcctt accgttcgga tactcagcag
    52861 ggaccgacgc cgcgtaaagt gaccgatgtt ctgcaaacgg gtcagcaaat ctgggttcgt
    52921 caggttggcg atgcatggtg gctggcacaa gtgccggaag tgaactcggc gctggtgtcg
    52981 atcaatccgc aaaatggtgc cgttatggcg ctggtcggtg gctttgattt caatcagagc
    53041 aagtttaacc gcgccaccca ggcactgcgt caggtgggtt ccaacatcaa accgttcctc
    53101 tacaccgcgg cgatggataa aggtctgacg ctggcaagta tgttgaacga tgtgccaatt
    53161 tctcgctggg atgcaggtgc cggttctgac tggcagccga agaactcacc accgcagtat
    53221 gctggtccaa ttcgcttacg tcaggggctg ggtcagtcga aaaacgtggt gatggtacgt
    53281 gcaatgcggg cgatgggcgt cgactacgct gcagaatatc tgcaacgctt cggcttcccg
    53341 gcgcaaaaca ttgtccacac cgaatcgctg gcgctgggtt cagcgtcctt caccccaatg
    53401 caggtggcgc gcggctacgc ggtcatggcg aacggcggct tcctagtgga cccgtggttt
    53461 atcagcaaaa ttgaaaacga tcagggcggc gtgattttcg aagcgaaacc gaaagtagcc
    53521 tgcccggaat gcgatattcc agtgatttac ggtgatacgc agaaatcgaa cgtgctggaa
    53581 aataacgatg ttgaagatgt cgctatctcc cgcgagcagc agaatgtttc tgtaccaatg
    53641 ccgcagctgg agcaggcaaa tcaggcgtta gtggcgaaga ctggcgcgca ggagtacgca
    53701 ccgcacgtca tcaacactcc gctggcattc ctgattaaga gtgctttgaa caccaatatc
    53761 tttggtgaac caggctggca gggtaccggc tggcgtgcag gtcgtgattt gcagcgtcgc
    53821 gatatcggcg ggaaaaccgg taccactaac agttcgaaag atgcgtggtt ctcgggttac
    53881 ggtccgggcg ttgtgacctc ggtctggatt ggctttgatg atcaccgtcg taatctcggt
    53941 catacaacgg cttccggagc gattaaagat cagatctcag gttacgaagg cggtgccaag
    54001 agtgcccagc ctgcatggga cgcttatatg aaagccgttc ttgaaggtgt gccggagcag
    54061 ccgctgacgc cgcctccggg tattgtgacg gtgaatatcg atcgcagcac cgggcagtta
    54121 gctaatggtg gcaacagccg cgaagagtat ttcatcgaag gtacgcagcc gacacaacag
    54181 gcggtgcacg aggtgggaac gaccattatc gataatggtg aggcacagga attgttctga
    54241 ttaaaaaggc gcttcggcgc cttttcagtt ggctgacaaa gtgcatttgt ttatgccgga
    54301 tgcgggtgaa cgcgttatcc ggctaacaaa atcgtgaaaa atcaatatat tgcaggaacc
    54361 ccataagcct gataagcgtt gtgcatcagg caaacttcac gcatttacac tcgcccctgc
    54421 cctttcaacc attcgcgcac gaggaacagc gcgctgacat tacgcgcttc gttgaagtca
    54481 gggtcttcca gcaaatccat catatgcgcc agcggccagc gcacctgtgg tagcggctct
    54541 ggctcatcgc cttccagcga ttccgggtag agatcttgcg cgaccacgat attcattttg
    54601 ctggaaaagt aagacggtgc catgctgagc ttcttcaaaa aagtcagatc gttcgcacca
    54661 aatccaacct cttcttttag ctcgcggtta gcggcttcgt agacgctttc acccggatca
    54721 attaatcctt tcgaaaaacc taattcgtag gattcagttc ccactgcgta ttcgcggatc
    54781 aggatcaggt gatcgtccac aatcggcaca atcatcactg cttcccggtt ggttggacgc
    54841 attcgttcat aaacacgccg cacgccattg ctgaactcca gatccacgct ctcgacggta
    54901 aacagtcggg aacgggctac agtttcaaca ttcagaatgg tgggtttttg taatgatttg
    54961 ttcatcgtgg gatctatgct gtgaaatcag cggttattgt gcgatatcgg acacgctttc
    55021 ggcaatgtga attgcatgtt atttacattt atgtaactta ataaataatc gtcctcaaat
    55081 caaattaaaa gtcaataggt tgaaataact ccaggaattt gctgatattc cgccttcaga
    55141 gggtttgcta tgatcagcgg ttactgtgat gtgcttaatg atgctcaagt taagctccac
    55201 gcttgccgat agccaaccgc agaatcatgt attgtgtccg gtgcgactga ccacgcctga
    55261 cagactaagt aagatgggga aagcatgagc accattgtga tttttttagc tgctttgctg
    55321 gcctgctcac tacttgcggg atggctgata aaagtgcgat ccagacggcg tcagctgccc
    55381 tggaccaacg ccttcgcgga tgcgcaaacg cgtaaactca cacctgaaga acgtagcgcc
    55441 gttgaaaatt atcttgagag cctgacgcag gtattacagg tgcctggccc aacggaagct
    55501 agcgcggcac cgatctctct ggcgctgaat gccgaaagca acaacgtcat gatgctgaca
    55561 cacgctatca cacgttacgg catctctacc gacgatccga ataaatggcg ttactacctc
    55621 gattcggtag aagtccacct gccccccttc tgggaacagt acatcaacga tgagaatacc
    55681 gttgaactga ttcataccga ttcgctgccg ttggttattt cactcaacgg tcatacgctg
    55741 caggagtaca tgcaggaaac gcgcggctat gccttgcaac ctgttccgtc cacgcaggcg
    55801 tcgattcgcg gggaagagag tgagcaaatc gagctactca atattcgcaa agaaacgcat
    55861 gaagaatatg cgctgagtcg tccgcgcggg ctgcgtgaag cgttgctgat cgtcgcctcc
    55921 ttcctgatgt tctttttctg cctgattacc ccggatgtat ttgttccgtg gctggcaggc
    55981 ggcgcattac tgctgctggg cgcaggtctg tgggggctat tcgcgccccc ggcaaaatcc
    56041 tccctgcgag aaattcattg tctgcgcggt acaccccgtc gttggggatt gtttggcgaa
    56101 aacgatcagg aacagatcaa caatatttca ctcggtatta tcgacctggt ctatcccgca
    56161 cactggcagc catacattgc tcaggatctc ggtcaacaaa ccgatattga tatctatctc
    56221 gaccgccacg tagtgcgtca ggggcgatat ctttcgctgc atgatgaagt aaaaaacttt
    56281 ccgttacagc actggctgcg cagtacgatt atcgctgcgg gctcgctgct agtgctgttt
    56341 atgctgttat tctggatccc gctggatatg ccgctgaaat tcactctctc atggatgaaa
    56401 ggcgcgcaga ccattgaagc caccagcgta aaacaactgg ctgacgctgg cgtgcgggta
    56461 ggcgatacat tgcgtattag cggtacggga atgtgtaata ttcgcacttc cggaacctgg
    56521 agcgcgaaaa ccaattcacc ttttttaccg tttgactgct cgcagatcat ctggaatgac
    56581 gcccgctcat taccattacc agaatctgaa ctggtcaaca aagcgacggc attgactgaa
    56641 gcagttaatc gccagctgca ccctaaaccg gaagatgaat ctcgcgtcag tgcctcatta
    56701 cgttcagcga ttcaaaaatc cggcatggta ttgcttgatg attttggcga cattgtactg
    56761 aagacagcgg atttatgttc tgccaaagat gactgtgtgc gactgaaaaa tgcgctggtc
    56821 aatctcggca acagtaaaga ctgggacgcg ctggtaaaac gcgccaacgc cgggaagctc
    56881 gatggcgtga atgtgttatt acgcccggtg agtgctgaat cgctggataa cctggtggca
    56941 acctccaccg cgccgtttat cacgcatgaa acggcgcgag cggcacaatc actaaacagt
    57001 ccggcccccg gcggattcct gattgtcagc gacgaaggca gcgattttgt tgatcagccc
    57061 tggccttcgg catcacttta cgactacccg ccgcaagaac agtggaacgc tttccagaaa
    57121 ctggcacaaa tgctgatgca tacgccgttt aacgccgaag gtatcgtcac aaaaatcttc
    57181 actgacgcca atggtacgca gcatattggc cttcatccga tcccggatcg ttccggcctg
    57241 tggcgctatc tcagcaccac attgctgcta ctgacgatgc tgggtagcgc catttacaat
    57301 ggcgtacagg cctggcgtcg ttaccagcgt catcgcactc gcatgatgga gattcaggcc
    57361 tattatgaaa gctgcctgaa cccgcaactg atcacccctt cagaaagcct tatcgaataa
    57421 cacgtttgcg cggcaggtta tgctaccctg tcgcgcaaat tgcttcactc tggagatttc
    57481 cctcatgcat atcaacattg cctggcagga cgtagatacc gttctgctgg atatggacgg
    57541 cacgttgctc gacctcgcct tcgataacta tttctggcaa aagctggtgc ctgaaacatg
    57601 gggcgcgaaa aacggggtta cgccacagga agcgatggaa tatatgcgcc agcaatatca
    57661 cgacgtacag catacgctaa actggtactg tcttgattac tggagtgagc aactgggtct
    57721 ggatatctgt gcgatgacca ccgagatggg accgcgcgcc gtactgcgtg aagataccat
    57781 tccgtttctt gaggcactga aagccagcgg taagcagcga attttgctca ccaatgctca
    57841 tccgcacaac ctggcggtaa aacttgagca tacgggtctg gacgcacacc ttgatttatt
    57901 actttccacc cacacatttg gttatccgaa agaggatcag cggttatggc atgcggtggc
    57961 cgaagctacg ggtctgaaag ctgaaagaac gctgtttatt gatgacagcg aagcgattct
    58021 cgatgctgcc gcgcaatttg gtattcgtta ctgcctcggc gtgactaatc ctgattccgg
    58081 gattgccgag aaacagtatc aacgccatcc gtcactgaat gactaccgcc gcctgatccc
    58141 ctcgctaatg tgaaggagac gccatgaaag agaaacctgc tgttgaggtt cgactggata
    58201 aatggctatg ggctgcccgt ttttataaaa cccgcgcgct ggcccgtgaa atgattgaag
    58261 gcggtaaggt gcattacaac gggcagcgca gcaagccgag caaaatcgtc gagctgaatg
    58321 ccacgctcac tctgcgccag ggaaatgacg aacgcacggt gattgtaaag gcgattactg
    58381 aacagcgtcg ccccgccagc gaggcagcct tgctgtatga agagactgcg gaaagtgtag
    58441 agaaacgcga aaaaatggcg atggcacgta aacttaatgc cttaaccatg ccgcacccgg
    58501 accgacgccc ggacaaaaaa gagcgccgcg acctgttacg atttaaacac ggcgacagtg
    58561 aataactgtc acctgcaaga gagatgatta tgccgcaaca tgaccaatta catcgctatc
    58621 tgtttgaaaa ttttgccgtt cgcggcgaac tggtaaccgt ttcggaaacc ctgcaacaga
    58681 tccttgagaa ccacgattat ccgcagcccg ttaaaaacgt gctggcagaa ctgctggttg
    58741 cgaccagcct gttaaccgct acgctgaagt ttgatggtga tatcaccgta cagctgcagg
    58801 gcgacggtcc gatgaatctg gcggttatta acggtaacaa taaccagcaa atgcgcggtg
    58861 tggcgcgcgt gcagggcgaa attccagaaa atgccgacct gaaaacgctg gtcggcaatg
    58921 gttacgtggt gatcaccatt accccgagcg aaggcgaacg ctatcagggc gtagttggtc
    58981 tggaaggtga taccctggcg gcctgcctag aagattactt tatgcgttct gaacagctgc
    59041 cgacgcgcct gtttattcgc accggcgacg tagacggcaa accggctgca ggcggtatgt
    59101 tgttgcaggt aatgcctgcg caaaataccc aacaggacga ctttgaccac ctggcgacgc
    59161 taaccgaaac catcaaaacc gaagaactgc tgaccttacc ggcaaacgaa gtgttgtggc
    59221 gtttgtatca cgaagaagag gtgacggttt acgatccgca ggatgtggag ttcaaatgca
    59281 cctgctcgcg tgaacgttgc gccgatgcgc tgaaaacgct gcctgatgaa gaagttgata
    59341 gcatcctggc ggaagatggc gaaattgaca tgcattgtga ttactgcggt aaccactatc
    59401 tgttcaatgc gatggatatt gctgaaatcc gcaacaacgc gtctccggca gatccgtaag
    59461 ttcattaatg agtatgtccg gcagagaggg ctctgccgga ttttacgact taatggcctg
    59521 atgtgctgcg cttaccagcg cctatgaaat aaatcgcaaa ttattttggt cagatacagc
    59581 gtcgtatccg ataaaagcgt tgtcacttac ttaagtttat ttccagcgtt tcaccatcta
    59641 ccgtcagatc gcgccacgct ttcggcctcg tccccagttt tccatccttc gcagctgtga
    59701 gtaatttttc tttcgttatt tcgttcggaa gtaaactcat atcaagcgcg tcccattctt
    59761 ttctgtctga gtcaaagcca tagacaatga ctctgtcatc attaaaagca aacaggatcc
    59821 gctccgcctg accatcacta ttcagatctt gctcgaccag tacgcaggcg tctttttcaa
    59881 tacaggtcat cacgttatag cgatcctgga ttaaggccga ccagaatgtc gcatccggtt
    59941 taacagaacc aggggcaatt aacacatttt cggcgaatac tttttccgat acctgttcct
    60001 gaagatgttg ctctccatct aacgccatca gcagatcccg cgcgcgtttc gggtctttca
    60061 taaacccggc atcgcttttc agcgactcaa gcgccgcacg tccatagcga ccgctctgtt
    60121 cgagcatgta gatagttacc tggtctggcg tgtttttgcc gctctgataa cgcgccatat
    60181 gactgttcac gctaatacgc atactgtcca gcaccggcga attaagaagc accagtatca
    60241 ccaacaccaa taacgaaacc gcaaggttca ctttgccctg aagaacaagg ggattttgcc
    60301 cattacgcca gacgatactg acaaaatacc ccagcgacca caccagtaac accagctccg
    60361 ccagcgcgcc ctgcaagcgg tcgacagtcc agccgtactg agcgacccgc agccataacc
    60421 cccaggtggc gacgaacaca tacagcggag caaccagcaa agcggtttta atcaggcaac
    60481 gcaacggccc cgtccaggga agtgacgctt tttgcggatc gcggacaata gccattaaga
    60541 tcaattgcaa aaaggccagc gttaacagca acccggcggc ggagatgtgg cgagaaatcg
    60601 cgctcaggcc cgtaaacggc agggtgatga taaacatcag ggttagcaat gatactaacg
    60661 gcagcaaccc cgtggcgatt agcgtgaaca acttttgaat agagtcgatt aaacgtgact
    60721 gtgtacgcgc gaggatcacc gccagtgcgg taaccagacc aaatgtaaga tacataaacc
    60781 agtcggttgc aaaaaagagc gtattgaaaa acgtgatgcc aacaagttta aacaactcac
    60841 cccacagtaa aagcaccagc cacgtcaggc cattggagag aaaaatcacc agtaatataa
    60901 gtacgttatg ccatactgac tggtaaaagt agctatagcg gctactgcca ttgcggatac
    60961 gaaggctttg ttgtatccac ggtagcaaca acattgccat caacagcaga tagcagccaa
    61021 aatcccaaat cgccttttca gctctccagg ggttcatgcc atcagtttgc cacttcagcc
    61081 aaccgctcat ccctgtcgtg gcaataaaca ccagcgccag ccatccctgt cgtggcaata
    61141 aacaccagcg ccagccatcc ccaaaggcgc ttctgtttaa aagagatcac ggagaagagt
    61201 aaaacggatg aaaacgcgac ggtcgcaggc acgccataaa caatccaact gtgatttttt
    61261 ccggcaagcc aggcgataag caggtagcaa accagcccct gaagtaatcc ggtagcaatc
    61321 atcccccagc gtgttgccgg tgaaagttca acgttatcca tgttggttat ccagaatcaa
    61381 aaggtgggtt aattatcgca tccgggcagt agtattttgc ttttttcaga aaataatcaa
    61441 aaaaagttag cgtggtgaat cgatacttta ccggttgaat ttgcatcaat ttcattcagg
    61501 aatgcgattc cactcacaat attcccgcca tataaaccaa gatttaacct tttgagaaca
    61561 ttttccacac ctaaaatgct atttctgcga taatagcaac cgtttcgtga caggaatcac
    61621 ggagtttttt gtcaaatatg aatttctcca gatacgtaaa tctatgagcc ttgtcgcggt
    61681 taacaccccc aaaaagactt tactattcag gcaatacata ttggctaagg agcagtgaaa
    61741 tgcgcgttaa caatggtttg cccccgcaag aactcgaggc ttatggtatc agtgacgtac
    61801 atgatatcgt ttacaaccca agctacgacc tgctgtatca ggaagagctc gatccgagcc
    61861 tgacaggtta tgagcgcggg gtgttaacta atctgggtgc cgttgccgtc gataccggga
    61921 tcttcaccgg tcgttcacca aaagataagt atatcgtccg tgacgatacc actcgcgata
    61981 ctttctggtg ggcagacaaa ggcaaaggta agaacgacaa caaacctctc tctccggaaa
    62041 cctggcagca tctgaaaggc ctggtgacca ggcagctttc cggcaaacgt ctgttcgttg
    62101 tcgacgcttt ctgtggtgcg aacccggata ctcgtctttc cgtccgtttc atcaccgaag
    62161 tggcctggca ggcgcatttt gtcaaaaaca tgtttattcg cccgagcgat gaagagctgg
    62221 caggtttcaa accagacttt atcgttatga acggcgcgaa gtgcactaac ccgcagtgga
    62281 aagaacaggg tctgaactcc gaaaacttcg tggcgtttaa cctgaccgag cgcatgcagc
    62341 tgattggcgg cacctggtac ggcggcgaaa tgaagaaagg gatgttctcg atgatgaact
    62401 acctgctgcc gctgaaaggt atcgcttcta tgcactgctc cgccaacgtt ggcgagaaag
    62461 gcgatgttgc ggtgttcttc ggcctttccg gcaccggtaa aaccaccctt tccaccgacc
    62521 cgaaacgccg cctgattggc gatgacgaac acggctggga cgatgacggc gtgtttaatt
    62581 tcgaaggcgg ctgctacgca aaaactatca agctgtcgaa agaagcggaa cctgaaatct
    62641 acaacgctat ccgtcgtgat gcgctgctgg aaaacgtcac cgtgcgtgaa gatggcacta
    62701 tcgactttga tgatggttca aaaaccgaga acacccgcgt ttcttatcca atctatcaca
    62761 tcgataacat cgttaagccg gtttccaaag cgggccacgc gactaaggtt atcttcctga
    62821 ctgctgatgc tttcggcgtg ttgccgccgg tttctcgcct gactgccgat caaacccagt
    62881 atcacttcct ctctggcttc accgccaaac tggccggtac tgagcgtggc atcaccgaac
    62941 cgacgccaac cttctccgct tgcttcggcg cggcgttcct gtcgctgcac ccgactcagt
    63001 acgcagaagt gctggtgaaa cgtatgcagg cggcgggcgc gcaggcttat ctggttaaca
    63061 ctggctggaa cggcactggc aaacgtatct cgattaaaga tacccgcgcc attatcgacg
    63121 ccatcctcaa cggttcgctg gataatgcag aaaccttcac tctgccgatg tttaacctgg
    63181 cgatcccaac cgaactgccg ggcgtagaca cgaagattct cgatccgcgt aacacctacg
    63241 cttctccgga acagtggcag gaaaaagccg aaaccctggc gaaactgttt atcgacaact
    63301 tcgataaata caccgacacc cctgcgggtg ccgcgctggt agcggctggt ccgaaactgt
    63361 aatgatttga agctggagaa tatctatcca gtatcttata gaaagcaaaa cgggaggcac
    63421 cttcgcctcc cgtttattta cccttctttt gtcgtgccct gcgcccgcgt taccggcact
    63481 ggcagccagg cgcgaatgga aagcccgccc cgctcgctgg tgccaagctc cagcatcccg
    63541 ttatggttat ccacgatacg ctgcacaatt gccagcccta atcccgtgcc gctaatggtg
    63601 cgcgcactgt cgccgcggac aaacggctgg aacaggtgct tacgttgttc cggcgcaatt
    63661 cccggaccgt catcttccac ctggaaccag gcgcgattcg gctccgttcc gctgctgact
    63721 ttgatccagc cattgccata acgggcggcg ttgaccacca tattcgccac cgcgcgtttg
    63781 atcgacagcg ggtgcatttt cacttcaatg ctgccggggt aaagcgcggt ttcaatttcc
    63841 cgctcatagc cactttcggc agcaatcacc tcaccgagca ctgcattaag atccgccatt
    63901 tccatcggca tctcctgccc ggtgcgcagg tagtcgataa actgctcaat gatggcgttg
    63961 cactcttcga tatctttatt gatcgattct gccagatagc catcctgctc gctcatcatc
    64021 tcagtcgcca ggcgaatacg cgtcagcggc gtgcgcaagt cgtgacttac ccccgccatc
    64081 agcagcgtgc ggtcatccgc cagttgctta acaccagccg ccatatggtt aaaggcacgg
    64141 gtaacggaac gcacctccga agcgccatat tcacgcagcg gcggcggaat aatcccttta
    64201 ccaacctgca aggctgcgtg ttcgagatcg accaacggtc ggttctggat acgaataaac
    64261 agccacgccc cgcctatcgc caatagcata atcgccagcg tatagcggaa cagcggagag
    64321 aaatcgccct gatgaatttc ggtcagcggc acgcgtaccc agatattggg cgacagccag
    64381 gttttcagcc agacgacagg cgaacttttg ttgacctcaa cgcgcacttc cgtcgggccg
    64441 cccagttgct gtgccatctg atggcttaag aattcatagt gttgcgccca acgcagacct
    64501 gcctcttcgg cagcctcgtt ggagtagaga gagatcccca gctcacggta gatctcccga
    64561 cggaaagcgg gaggcacaac caactgcgtg ccgtcctcca gttgcagttt gtcggtcatc
    64621 aacatacgca cttcgtacgc gaggacttta ttaaactgct ggaggctcgg caaaatcgcg
    64681 aagttcagca ccaccagata agtcgtcacc aggctggcga acagcaaggt gacgatgagc
    64741 aataacgtac gggcaaatga acttcgtggc gagaagcgca atcgcctcat gctttagagc
    64801 cgtccggtac aaagacgtag cccaggcccc aaacggtctg aatgtaacgc ggatgcgctg
    64861 gatcttcttc taccatgcgg cgcagacgcg aaatctgcac gtcgatggag cgttccattg
    64921 cggaatattc acgaccacgg gcaaggttca tcagcttatc gcgggagagc ggctcacgcg
    64981 gatggctgac cagtgccttc agtaccgcaa actcaccgct ggtgagcggc atcggctcat
    65041 cttcgcggaa catttcgcgc gtaccgaggt taagtttgaa cttaccgaaa gcaattaccg
    65101 cctcttcctg tgacggtgcg cctggcagtt cgttcgcctg acgacgcagc accgcacgaa
    65161 tacgggccag cagttcacgc gggttaaacg gttttggaat gtagtcgtca gcgccaatct
    65221 ccaggcctac gatacggtcc acttcttccc ctttcgccgt caccataatg atcggcatcg
    65281 ggttgctctg actacgaaga cgtcggcaaa tcgacaagcc atcttcacca ggtaacatta
    65341 aatccagtac cataagatgg aaagattcac gagtcagcag gcgatccatc tgttctgcat
    65401 tagcgacgct tcgaacctgg aagccttgtt cggtgagata acgttccagc agcgcacgca
    65461 ggcgcatgtc gtcatcgacc accagaatct tgtagttctc ttgcattgtt tgtactccca
    65521 aaggttcgca acaatttgta agcgtgtatt cttaaaaaag ctcacgttcg tcaccagcta
    65581 aatctggtat gaatttcagc ctaaattgtt acaaagcata ttaaacagca gcttaagtat
    65641 acaatttatt cggcgaaaca ttattgattc tgttgatatg atcacgttat acccaatgtg
    65701 cgcattatca aacagacaaa gggaatcaac gagatgaaaa cgcccctggt tacccgggaa
    65761 gggtatgaaa aactcaaaca agagcttaat tatctctggc gtgaagaacg cccggaggtc
    65821 acaaaaaagg tgacctgggc cgcaagtctg ggcgaccgca gcgaaaatgc tgactatcag
    65881 tataataaaa agcgtctgcg tgaaatcgac cgtcgcgtgc gctatctcac taaatgcctg
    65941 gaaaatctca aaatcgtcga ttactcccct cagcaggaag gcaaagtctt ttttggcgcg
    66001 tgggtggaga ttgaaaacga cgatggcgtg actcaccgtt tccgtattgt cggctacgat
    66061 gaaatttttg gccgtaaaga ttacatctct atcgattccc cgatggcccg cgcattgctg
    66121 aaaaaagaag tcggcgatct ggcggtggtg aatacccctg ccggggaagc gaactggtat
    66181 gttaatgcta tcgagtacgt gaaaccgtaa ggaagagtct taacctcctg ccgatggctg
    66241 gcatttttgc cagccagtcc gtataactat cccctgattt ttgatccgaa aagatgaact
    66301 caaaccatga tgaatgattc gttctgccgc attattgcgg gtgaaattca ggcgcgcccg
    66361 gaacaggttg acgctgccgt tcgcctgctt gacgaaggga ataccgtgcc gtttatcgca
    66421 cgttatcgta aggaaatcac cggcggtctg gatgacacgc agctgcgtaa tctggaaacg
    66481 cgtctgagct atctgcgcga gctggaagag agacgtcagg cgatcctcaa atccatttcc
    66541 gagcaaggca aactcaccga tgatctggcg aaggccatca acgccaccct aagcaaaacc
    66601 gaactcgaag acctctacct gccctacaaa cctaaacgcc gcacccgcgg gcaaatcgcc
    66661 attgaagcag ggcttgagcc gttggctgac ctgctgtgga gcgatccgtc acacacgcca
    66721 gaagtcgccg ctgcacaata tgttgatgcc gataaaggcg tggcagatac caaagccgcg
    66781 ctggatggcg cgcgctatat cctgatggaa cggtttgccg aagatgccac gctgctggcg
    66841 aaagtgcgtg attatctgtg gaagaacgcg catttggttt ctacggtggt gagcggtaaa
    66901 gaagaggaag gggcgaaatt ccgcgactat ttcgatcatc acgaaccgtt gtccacggtg
    66961 ccttctcacc gcgcgctggc gatgttccgt gggcgtaacg aaggcgtact ccagctttcg
    67021 ctgaatgccg atccgcagtt cgatgagccg cccaaagaga gctattgcga gcaaatcatc
    67081 atggatcacc ttggcctgcg cctgaacaat gccccggcgg atagctggcg caaaagcgta
    67141 gtgagctgga cctggcgcat caaggtgctg atgcatctgg aaaccgaact gatgggtacc
    67201 gtgcgcgaac gcgcggaaga tgaagcaatc aacgtctttg cccgtaacct gcacgatctg
    67261 ctgatggcgg cccctgccgg actgcgtgca acgatgggcc tcgatccggg tctgcgtacc
    67321 ggggtaaaag tggcggtggt cgatgccact ggcaaactgg tggcgaccga taccatttac
    67381 ccgcacaccg gacaagccgc aaaagcagcg atgaccgttg ctgccttgtg tgaaaaacat
    67441 aacgttgaac tggtggcgat cggcaacggt acagcttccc gcgaaactga acgtttctat
    67501 ctcgacgtgc agaagcagtt cccgaaagtg accgcacaga aagtgatcgt cagcgaagct
    67561 ggcgcgtcgg tttactcagc ttccgagctg gcagcgcagg agttcccgga tctcgacgtt
    67621 tcgctgcgtg gtgcggtatc tatcgcccgc cgtttgcagg atccgctggc ggagctggtg
    67681 aaaatcgatc cgaaatctat cggcgtaggt cagtatcagc atgacgtcag ccagacgcaa
    67741 ctggcccgca aactggatgc agtagtagaa gactgcgtaa acgccgttgg cgtcgatctc
    67801 aacaccgctt ctgttccgct attaacccgc gtggcgggcc tgacgcgcat gatggcgcaa
    67861 aacatcgtgg cctggcgcga tgagaacggc cagttccaga accgtcagca actgctgaaa
    67921 gtcagccgtc tggggccgaa agccttcgag cagtgcgcgg gcttcctgcg cattaaccac
    67981 ggtgataacc cgctggacgc ctctaccgtt catccggaag cctatccggt ggtggaacgc
    68041 attctggcag caacacagca ggcactgaaa gatctgatgg gtaacagcag cgaactgcgt
    68101 aacctgaaag cgtctgactt tactgatgaa aaattcggtg tgccgacagt aactgacatc
    68161 atcaaagagc tggaaaaacc gggtcgcgat ccgcgtccgg aatttaaaac cgctcagttt
    68221 gccgatggcg tcgagacaat gaacgacctg caaccgggta tgatcctcga aggcgcagtg
    68281 accaacgtca ccaactttgg cgcgtttgtc gatattggcg tgcatcagga cggcctggtt
    68341 cacatctctt cattgtcgaa caagtttgtg gaagatccgc ataccgtggt gaaagcgggc
    68401 gacattgtga aggtgaaagt gctggaagtg gatcttcagc gtaaacgtat cgccctgact
    68461 atgcgtctgg acgagcagcc tggcgaaacc aactctcgtc gcggcggcgg taatgaacgc
    68521 ccgcaaaaca accgcccggc agccaaacca cgcggtcgtg aagcgcagcc tgccggtaat
    68581 agcgcgatga tggatgcgct ggcggcagcg atgggtaaaa aacgttaaac gcccggactg
    68641 gcccctaaac ctgcgcgata aggcgttcac gccgcatctg gcaacgaacg ctttgcctga
    68701 cataaaagtg ccggagaata tctccggcac ttttattcca cagccaaact cacaatatac
    68761 tccgttaata tttatcactt cattaacaac tgaaacctta attaaacatt agccagtccg
    68821 ggtaactcac tattcgaatt atattttcgc tgcgatataa ccttgagcca catcaacatt
    68881 gagtcagatt attattcaaa ccaacattcg cacacatttt aagtattgct gatagaaacc
    68941 attctcatta tcattgtgtt gttgattatt taatctctcc tttgttggca aatcatctgg
    69001 tctcatgtcg cggtcaaacg ccccatgagg tagttatcca gttaatgaga aacaagtagg
    69061 cacctatgca atacactcca gatactgcgt ggaaaatcac tggcttttcc cgtgaaatca
    69121 gcccggcata tcgccaaaaa ctgctttctc taggcatgtt acctggctcc tcttttgatg
    69181 tggtgcgcgt cgctccactc ggcgacccca ttcatatcga aacccgtcgt gtgagcctgg
    69241 tattacgcaa aaaagatctg gccttattag aagtggaagc ggtttcctgt taatacggtg
    69301 ataacaacaa tgaaaaaatt aaccattggc ttaattggta atccaaattc tggcaagaca
    69361 accttattta accagctcac tggcgcacgt cagcgtgtag gtaactgggc tggcgttacc
    69421 gtcgaacgta aagaagggca attctccacc accgatcatc aggtcacgct ggtggacctg
    69481 cccggcacct attctctgac caccatctca tcgcagacct cgctcgatga gcaaatcgcc
    69541 tgtcactaca ttttgagtgg cgacgccgac ctgctgatta acgtggtgga tgcgtctaac
    69601 cttgagcgta acctgtacct gacgctgcaa ctgctggaac tcggcattcc ctgcatcgtg
    69661 gcgctgaaca tgctcgacat tgccgagaag caaaatattc gtattgaaat tgatgctcta
    69721 tcggcacgtc ttggctgccc ggtgatcccg ctggtttcaa cccgtggtcg cggtattgaa
    69781 gcgctcaagc tggcgattga tcgctataaa gctaacgaga atgtggaact ggtgcattac
    69841 gcacagccgc tactcaacga agcagattca ctggcaaaag tgatgccttc cgacatcccg
    69901 ctgaaacaac gtcgctggct gggcctgcaa atgctggaag gcgatatcta cagccgcgcc
    69961 tacgccggtg aagcgtcgca gcatctggat gccaccctcg cccgtctgcg taatgaaatg
    70021 gatgatccgg cgctgcacat tgccgatgcg cgttaccagt gcattgctgc catctgtgat
    70081 gtggtaagca acaccctgac ggcagaaccc agccgtttca ccactgcggt agataaaatc
    70141 gtgctcaacc gtttcctcgg tctgccgatt ttcctctttg tgatgtacct gatgttcctg
    70201 ctggctatca acatcggcgg ggcgttacag ccgctgttta acgtcggctc cgtggcgcta
    70261 tttgtgcatg gtattcaatg gattggctac acgctccact tcccggactg gctgactatc
    70321 ttcctcgccc agggcctggg tggcggtatt aacaccgtgc tgccactggt gccgcagatc
    70381 ggcatgatgt acctgttcct ctccttcctt gaggactccg gctatatggc gcgtgcagcg
    70441 tttgtgatgg accgcctgat gcaggcgctg ggcttgccgg ggaaatcctt tgtgccgctg
    70501 atcgtcggtt tcggttgtaa cgtaccgtcg gtaatgggcg cacgtacgct tgatgcaccg
    70561 cgtgaacgtc tgatgaccat catgatggca ccgtttatgt cctgcggcgc gcgtctggct
    70621 atcttcgcag tattcgcggc tgccttcttc gggcagaacg gtgccctggc ggttttctcg
    70681 ctgtatatgc tcggtatcgt gatggcggtg ctgactggcc tgatgctcaa gtacaccatc
    70741 atgcgcggtg aagcgacgcc gtttgtcatg gagctgccgg tctaccatgt accacacgtt
    70801 aaaagcctga ttatccagac ctggcagcgt ctgaagggct tcgttctgcg tgctggtaaa
    70861 gtgattatca tcgtcagcat tttcctgagc gctttcaaca gcttctcgtt gagcgggaaa
    70921 atcgtcgata acatcaacga ctcggcgctg gcttccgtca gccgggtgat aacccctgtc
    70981 ttcaagccga ttggcgtaca tgaagataac tggcaggcaa cggttggcct gtttacgggc
    71041 gcaatggcaa aagaagtggt ggtgggtacg ctcaacaccc tctacaccgc agaaaatatt
    71101 caggacgaag agttcaatcc ggcggaattc aacctcggtg aagagctgtt cagtgcggta
    71161 gatgaaacct ggcagagcct gaaagacacc ttcagcctta gcgtactgat gaaccccatt
    71221 gaagctagca aaggcaacgg tgaaatgggt accggggcga tgggcgtgat ggatcagaaa
    71281 ttcggtagcg cagctgccgc atacagctac ctgattttcg tcctgctgta tgtaccatgt
    71341 atctcggtga tgggagccat cgcccgtgaa tcaagccgtg gctggatggg cttctccatc
    71401 ctgtgggggc tgaatatcgc ttactcactg gcaacattgt tctatcaggt cgccagctac
    71461 agtcagcacc caacttacag cctggtgtgc attctggcgg taatcctgtt taacatcgta
    71521 gctatcggtc tgctgcgccg cgcgcgtagc cgggtggata tcgaactgct ggcaacccgc
    71581 aagtcggtaa gcagttgctg cgcagccagc accaccggtg attgccatta atggcttcac
    71641 ttattcaggt acgtgatttg ctggcgttac ggggccgtat ggaagcgacc cagataagcc
    71701 agacattgaa cactccgcag ccaatgatta acgccatgct gcaacagctg gaaagcatgg
    71761 gcaaagccgt gcggattcag gaagaacctg acggctgcct ctctggcagt tgtaaaagct
    71821 gcccggaagg caaagcctgt ctgcgcgagt ggtgggcgct gcgttaacca tactccatcg
    71881 cctgttttga aaagcccggt atgcgtttgc atccgggctt ttttgcgtgc ggctttccat
    71941 aaaaatgcaa ttcttgcagc gcaacgtaag ttcctctgaa agcgtctcgc agggatgaaa
    72001 actcgctaat gcacagatgt ggagtggcgc gtagagtcgc ggcattcaaa caacaggtga
    72061 aggaacgcca tgagtaaaaa gcagagttcc accccacatg atgcgctgtt caaactcttt
    72121 ttacgccaac cggagacggc acgcgatttt cttgcgtttc atttaccggc acccattcac
    72181 gcgctctgtg atatgaaaac cctcaagctg gagtcgagga gctttattga tgacgatctg
    72241 cgtgaaagct attccgatgt gctgtggtcg gtgaaaacgg aacaaggacc aggatacatc
    72301 tattgtctga ttgaacatca aagcacctca aacaaactga tcgcatttcg catgatgcgt
    72361 tacgctattg ccgcaatgca aaatcacctt gatgctggat acaaaacgtt gccgatggtg
    72421 gtgccattgt tgttttacca cggtattgaa agcccctatc cctattcgct gtgttggctg
    72481 gattgtttcg ccgatcccaa actggcaagg cagctttatg cctccgcatt tccgctgatt
    72541 gatgtcaccg tcatgcctga tgatgaaatc atgcagcacc gacgtatggc gctgctggag
    72601 ttaattcaaa aacatattcg tcaacgcgat ctgatggggc tggtagagca aatggcctgc
    72661 ttattaagta gtggatacgc taatgacaga caaatcaaag ggctgtttaa ttacatactg
    72721 caaactggcg acgctgtacg ttttaacgat tttatcgacg gcgttgccga acgttcaccg
    72781 aaacacaagg agagtttaat gactattgcg gaaagattgc ggcaggaggg ggaacaatcc
    72841 aaagccctgc atatagccaa aataatgctt gaatccggag tccctcttgc agacatcatg
    72901 cgatttaccg ggctgtcaga agaagagttg gctgcggcga gtagactggc cccctgaatc
    72961 tccagacaac cagtatcact taa
//
LOCUS       DAZPCS010000002        70712 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZPCS010000002 DAZPCS010000000
VERSION     DAZPCS010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 70712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70712
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.002"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..795
                     /locus_tag="ACHUQ7_000074"
     CDS             <1..795
                     /locus_tag="ACHUQ7_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR1133669.1"
                     /translation="WLPRSLVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLD
                     DDNNHNSSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAF
                     ALIDANGASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQ
                     FVVPNRGAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILY
                     LSGISSKSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            806..1897
                     /locus_tag="ACHUQ7_000075"
     CDS             806..1897
                     /locus_tag="ACHUQ7_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1133670.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(1940..2800)
                     /gene="rsmI"
                     /locus_tag="ACHUQ7_000076"
     CDS             complement(1940..2800)
                     /gene="rsmI"
                     /locus_tag="ACHUQ7_000076"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFR1133671.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            2865..4901
                     /gene="lpoA"
                     /locus_tag="ACHUQ7_000077"
     CDS             2865..4901
                     /gene="lpoA"
                     /locus_tag="ACHUQ7_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFR1133672.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADSVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSALGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            4859..5254
                     /locus_tag="ACHUQ7_000078"
     CDS             4859..5254
                     /locus_tag="ACHUQ7_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFR1133673.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            5274..5864
                     /gene="diaA"
                     /locus_tag="ACHUQ7_000079"
     CDS             5274..5864
                     /gene="diaA"
                     /locus_tag="ACHUQ7_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFR1133674.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            5874..6449
                     /gene="dolP"
                     /locus_tag="ACHUQ7_000080"
     CDS             5874..6449
                     /gene="dolP"
                     /locus_tag="ACHUQ7_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFR1133675.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(6563..7603)
                     /locus_tag="ACHUQ7_000081"
     CDS             complement(6563..7603)
                     /locus_tag="ACHUQ7_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFR1133676.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(7676..8311)
                     /locus_tag="ACHUQ7_000082"
     CDS             complement(7676..8311)
                     /locus_tag="ACHUQ7_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFR1133677.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            8439..8957
                     /gene="yhbO"
                     /locus_tag="ACHUQ7_000083"
     CDS             8439..8957
                     /gene="yhbO"
                     /locus_tag="ACHUQ7_000083"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFR1133678.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(8937..9380)
                     /locus_tag="ACHUQ7_000084"
     CDS             complement(8937..9380)
                     /locus_tag="ACHUQ7_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFR1133679.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            9431..9733
                     /gene="yhbQ"
                     /locus_tag="ACHUQ7_000085"
     CDS             9431..9733
                     /gene="yhbQ"
                     /locus_tag="ACHUQ7_000085"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFR1133680.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(9720..10223)
                     /locus_tag="ACHUQ7_000086"
     CDS             complement(9720..10223)
                     /locus_tag="ACHUQ7_000086"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR1133681.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFLVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(10217..10741)
                     /locus_tag="ACHUQ7_000087"
     CDS             complement(10217..10741)
                     /locus_tag="ACHUQ7_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFR1133682.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNTQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            10950..11945
                     /locus_tag="ACHUQ7_000088"
     CDS             10950..11945
                     /locus_tag="ACHUQ7_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFR1133683.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQAAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            11954..12832
                     /locus_tag="ACHUQ7_000089"
     CDS             11954..12832
                     /locus_tag="ACHUQ7_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFR1133684.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12913..13920
                     /locus_tag="ACHUQ7_000090"
     CDS             12913..13920
                     /locus_tag="ACHUQ7_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFR1133685.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHLPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYDV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(14038..15282)
                     /gene="mtr"
                     /locus_tag="ACHUQ7_000091"
     CDS             complement(14038..15282)
                     /gene="mtr"
                     /locus_tag="ACHUQ7_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFR1133686.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(15436..17325)
                     /gene="deaD"
                     /locus_tag="ACHUQ7_000092"
     CDS             complement(15436..17325)
                     /gene="deaD"
                     /locus_tag="ACHUQ7_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFR1133687.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(17318..17398)
                     /gene="yrbN"
                     /locus_tag="ACHUQ7_000093"
     CDS             complement(17318..17398)
                     /gene="yrbN"
                     /locus_tag="ACHUQ7_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFR1133688.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(17505..18389)
                     /gene="nlpI"
                     /locus_tag="ACHUQ7_000094"
     CDS             complement(17505..18389)
                     /gene="nlpI"
                     /locus_tag="ACHUQ7_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFR1133689.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(18498..20633)
                     /gene="pnp"
                     /locus_tag="ACHUQ7_000095"
     CDS             complement(18498..20633)
                     /gene="pnp"
                     /locus_tag="ACHUQ7_000095"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFR1133690.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20880..21149)
                     /gene="rpsO"
                     /locus_tag="ACHUQ7_000096"
     CDS             complement(20880..21149)
                     /gene="rpsO"
                     /locus_tag="ACHUQ7_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFR1133691.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(21298..22242)
                     /gene="truB"
                     /locus_tag="ACHUQ7_000097"
     CDS             complement(21298..22242)
                     /gene="truB"
                     /locus_tag="ACHUQ7_000097"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFR1133692.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERIVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(22242..22643)
                     /gene="rbfA"
                     /locus_tag="ACHUQ7_000098"
     CDS             complement(22242..22643)
                     /gene="rbfA"
                     /locus_tag="ACHUQ7_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFR1133693.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(22807..25455)
                     /gene="infB"
                     /locus_tag="ACHUQ7_000099"
     CDS             complement(22807..25455)
                     /gene="infB"
                     /locus_tag="ACHUQ7_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFR1133694.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(25480..26967)
                     /gene="nusA"
                     /locus_tag="ACHUQ7_000100"
     CDS             complement(25480..26967)
                     /gene="nusA"
                     /locus_tag="ACHUQ7_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFR1133695.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSAELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26995..27447)
                     /gene="rimP"
                     /locus_tag="ACHUQ7_000101"
     CDS             complement(26995..27447)
                     /gene="rimP"
                     /locus_tag="ACHUQ7_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFR1133696.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(27654..27730)
                     /locus_tag="ACHUQ7_000102"
     tRNA            complement(27654..27730)
                     /locus_tag="ACHUQ7_000102"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27694..27696),aa:Met,seq:cat)
     gene            28078..29421
                     /gene="argG"
                     /locus_tag="ACHUQ7_000103"
     CDS             28078..29421
                     /gene="argG"
                     /locus_tag="ACHUQ7_000103"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFR1133697.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGLPQVENMENKGQ"
     gene            complement(29429..31055)
                     /locus_tag="ACHUQ7_000104"
                     /pseudo
     CDS             complement(29429..31055)
                     /locus_tag="ACHUQ7_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(31514..31600)
                     /locus_tag="ACHUQ7_000105"
     tRNA            complement(31514..31600)
                     /locus_tag="ACHUQ7_000105"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31564..31566),aa:Leu,seq:gag)
     gene            complement(31615..31947)
                     /gene="secG"
                     /locus_tag="ACHUQ7_000106"
     CDS             complement(31615..31947)
                     /gene="secG"
                     /locus_tag="ACHUQ7_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFR1133698.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNRGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(32175..33512)
                     /gene="glmM"
                     /locus_tag="ACHUQ7_000107"
     CDS             complement(32175..33512)
                     /gene="glmM"
                     /locus_tag="ACHUQ7_000107"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFR1133699.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(33505..34353)
                     /gene="folP"
                     /locus_tag="ACHUQ7_000108"
     CDS             complement(33505..34353)
                     /gene="folP"
                     /locus_tag="ACHUQ7_000108"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFR1133700.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(34443..36377)
                     /gene="ftsH"
                     /locus_tag="ACHUQ7_000109"
     CDS             complement(34443..36377)
                     /gene="ftsH"
                     /locus_tag="ACHUQ7_000109"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFR1133701.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(36477..37106)
                     /gene="rlmE"
                     /locus_tag="ACHUQ7_000110"
     CDS             complement(36477..37106)
                     /gene="rlmE"
                     /locus_tag="ACHUQ7_000110"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFR1133702.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            37232..37525
                     /gene="yhbY"
                     /locus_tag="ACHUQ7_000111"
     CDS             37232..37525
                     /gene="yhbY"
                     /locus_tag="ACHUQ7_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFR1133703.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(37681..38157)
                     /gene="greA"
                     /locus_tag="ACHUQ7_000112"
     CDS             complement(37681..38157)
                     /gene="greA"
                     /locus_tag="ACHUQ7_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFR1133704.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            38405..39838
                     /gene="dacB"
                     /locus_tag="ACHUQ7_000113"
     CDS             38405..39838
                     /gene="dacB"
                     /locus_tag="ACHUQ7_000113"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFR1133705.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPSDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     KPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            39902..40003
                     /locus_tag="ACHUQ7_000114"
                     /pseudo
     CDS             39902..40003
                     /locus_tag="ACHUQ7_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(40024..41196)
                     /gene="cgtA"
                     /locus_tag="ACHUQ7_000115"
     CDS             complement(40024..41196)
                     /gene="cgtA"
                     /locus_tag="ACHUQ7_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFR1133706.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(41212..42177)
                     /locus_tag="ACHUQ7_000116"
     CDS             complement(41212..42177)
                     /locus_tag="ACHUQ7_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR1133707.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(42304..42561)
                     /gene="rpmA"
                     /locus_tag="ACHUQ7_000117"
     CDS             complement(42304..42561)
                     /gene="rpmA"
                     /locus_tag="ACHUQ7_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFR1133708.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(42582..42893)
                     /gene="rplU"
                     /locus_tag="ACHUQ7_000118"
     CDS             complement(42582..42893)
                     /gene="rplU"
                     /locus_tag="ACHUQ7_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFR1133709.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            43151..44122
                     /gene="ispB"
                     /locus_tag="ACHUQ7_000119"
     CDS             43151..44122
                     /gene="ispB"
                     /locus_tag="ACHUQ7_000119"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFR1133710.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            44349..44627
                     /gene="sfsB"
                     /locus_tag="ACHUQ7_000120"
     CDS             44349..44627
                     /gene="sfsB"
                     /locus_tag="ACHUQ7_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFR1133711.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(44675..45934)
                     /gene="murA"
                     /locus_tag="ACHUQ7_000121"
     CDS             complement(44675..45934)
                     /gene="murA"
                     /locus_tag="ACHUQ7_000121"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFR1133712.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(45989..46243)
                     /gene="ibaG"
                     /locus_tag="ACHUQ7_000122"
     CDS             complement(45989..46243)
                     /gene="ibaG"
                     /locus_tag="ACHUQ7_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFR1133713.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(46403..46696)
                     /gene="mlaB"
                     /locus_tag="ACHUQ7_000123"
     CDS             complement(46403..46696)
                     /gene="mlaB"
                     /locus_tag="ACHUQ7_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFR1133714.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(46696..47331)
                     /gene="mlaC"
                     /locus_tag="ACHUQ7_000124"
     CDS             complement(46696..47331)
                     /gene="mlaC"
                     /locus_tag="ACHUQ7_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFR1133715.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(47350..47901)
                     /gene="mlaD"
                     /locus_tag="ACHUQ7_000125"
     CDS             complement(47350..47901)
                     /gene="mlaD"
                     /locus_tag="ACHUQ7_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFR1133716.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTESTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(47906..48688)
                     /gene="mlaE"
                     /locus_tag="ACHUQ7_000126"
     CDS             complement(47906..48688)
                     /gene="mlaE"
                     /locus_tag="ACHUQ7_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFR1133717.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(48696..49505)
                     /gene="mlaF"
                     /locus_tag="ACHUQ7_000127"
     CDS             complement(48696..49505)
                     /gene="mlaF"
                     /locus_tag="ACHUQ7_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFR1133718.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            49715..50680
                     /locus_tag="ACHUQ7_000128"
     CDS             49715..50680
                     /locus_tag="ACHUQ7_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFR1133719.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTREQLA
                     ELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMALTAIAIGTSL
                     PELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVML
                     LVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            50694..51680
                     /gene="kdsD"
                     /locus_tag="ACHUQ7_000129"
     CDS             50694..51680
                     /gene="kdsD"
                     /locus_tag="ACHUQ7_000129"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFR1133720.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPSEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            51701..52267
                     /gene="kdsC"
                     /locus_tag="ACHUQ7_000130"
     CDS             51701..52267
                     /gene="kdsC"
                     /locus_tag="ACHUQ7_000130"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFR1133721.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            52264..52839
                     /gene="lptC"
                     /locus_tag="ACHUQ7_000131"
     CDS             52264..52839
                     /gene="lptC"
                     /locus_tag="ACHUQ7_000131"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFR1133722.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            52808..53365
                     /gene="lptA"
                     /locus_tag="ACHUQ7_000132"
     CDS             52808..53365
                     /gene="lptA"
                     /locus_tag="ACHUQ7_000132"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFR1133723.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            53372..54097
                     /gene="lptB"
                     /locus_tag="ACHUQ7_000133"
     CDS             53372..54097
                     /gene="lptB"
                     /locus_tag="ACHUQ7_000133"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFR1133724.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            54145..55578
                     /gene="rpoN"
                     /locus_tag="ACHUQ7_000134"
     CDS             54145..55578
                     /gene="rpoN"
                     /locus_tag="ACHUQ7_000134"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFR1133725.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            55601..55888
                     /gene="hpf"
                     /locus_tag="ACHUQ7_000135"
     CDS             55601..55888
                     /gene="hpf"
                     /locus_tag="ACHUQ7_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFR1133726.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            56006..56497
                     /gene="ptsN"
                     /locus_tag="ACHUQ7_000136"
     CDS             56006..56497
                     /gene="ptsN"
                     /locus_tag="ACHUQ7_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFR1133727.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            56543..57397
                     /gene="rapZ"
                     /locus_tag="ACHUQ7_000137"
     CDS             56543..57397
                     /gene="rapZ"
                     /locus_tag="ACHUQ7_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFR1133728.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            57394..57666
                     /gene="npr"
                     /locus_tag="ACHUQ7_000138"
     CDS             57394..57666
                     /gene="npr"
                     /locus_tag="ACHUQ7_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFR1133729.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            57879..58511
                     /gene="yrbL"
                     /locus_tag="ACHUQ7_000139"
     CDS             57879..58511
                     /gene="yrbL"
                     /locus_tag="ACHUQ7_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFR1133730.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(58508..59235)
                     /gene="mtgA"
                     /locus_tag="ACHUQ7_000140"
                     /pseudo
     CDS             complement(58508..59235)
                     /gene="mtgA"
                     /locus_tag="ACHUQ7_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
     gene            complement(59232..59885)
                     /gene="elbB"
                     /locus_tag="ACHUQ7_000141"
     CDS             complement(59232..59885)
                     /gene="elbB"
                     /locus_tag="ACHUQ7_000141"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFR1133731.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(60115..62451)
                     /gene="arcB"
                     /locus_tag="ACHUQ7_000142"
     CDS             complement(60115..62451)
                     /gene="arcB"
                     /locus_tag="ACHUQ7_000142"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFR1133732.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(62547..63476)
                     /locus_tag="ACHUQ7_000143"
     CDS             complement(62547..63476)
                     /locus_tag="ACHUQ7_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709009.2"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFR1133733.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETSVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            64151..68611
                     /gene="gltB"
                     /locus_tag="ACHUQ7_000144"
     CDS             64151..68611
                     /gene="gltB"
                     /locus_tag="ACHUQ7_000144"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFR1133734.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            68624..70042
                     /gene="gltD"
                     /locus_tag="ACHUQ7_000145"
     CDS             68624..70042
                     /gene="gltD"
                     /locus_tag="ACHUQ7_000145"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFR1133735.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            70226..70630
                     /locus_tag="ACHUQ7_000146"
                     /pseudo
     CDS             70226..70630
                     /locus_tag="ACHUQ7_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417687.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 N-terminal domain-containing protein"
BASE COUNT        17316 a        19176 c        17559 g        16661 t
ORIGIN      
        1 tggttaccgc gtagcctggt ttcctatcag atgaccagcc agaaagatcg cccaacccaa
       61 catgaaatgc gtcttgatgg ctcactgctg gatgatggtc gcctgagcta tagcctggaa
      121 caaagtctgg atgacgataa caaccataac agtagcctga acgccagtta ccgttcacct
      181 tatggaacct tcagtgccgg atacagttac ggtaatgaca gcagccaata caattacggc
      241 gttaccggcg gcgtggttat ccatcctcat ggcgtgacgc tctcgcaata tctgggcaac
      301 gcttttgcgc ttatcgatgc taacggggct tctggcgtga ggatacaaaa ctatccgggg
      361 attgctactg atccctttgg ctatgcagtg gttccttatc tcacgactta tcaggaaaac
      421 cgtctctcgg tagatactac gcagctgccc gataacgtcg atcttgaaca aacaacacag
      481 tttgtggtgc ccaacagagg tgcaatggta gcggcgcgtt tcaacgccaa tatcggttat
      541 cgcgtacttg ttacagtcag cgatcgcaac ggtaaaccgt tgccctttgg cgctcttgcc
      601 agcaacgatg atacggggca acaaagtatc gtcgatgagg gcggcatact atatctctct
      661 gggatatcga gtaaatcaca aagctggact gtacgctggg gaaatcaggc agatcaacaa
      721 tgtcagtttg cttttagtac accggattca gaaccaacaa cctctgtatt acaaggcaca
      781 gcgcagtgcc attaaggata aaaaaatgaa aagagcgcct cttataacag gacttttgtt
      841 gatatccaca tcctgcgctt atgcctcctc aggagggtgt ggagccgaca gcactagcgg
      901 tgcgacaaat tacagcagtg tggttgatga tgttacggtg aaccagacag ataacgtgac
      961 aggacgggag tttacctctg caacgctaag tagcactaac tggcaatacg cctgttcctg
     1021 ctctgcgggt aaggcagtta aacttgtcta tatggtcagc cccgtactta ccaccactgg
     1081 acatcagaca ggatattaca aactcaatga cagcctggat attaaaacca cattacaggc
     1141 aaacgacatt ccaggactca caaccgacca ggttgtctct gttaacaccc gattcacaca
     1201 gataaaaagc agcaccgtat attctgctgc aacccaaacg ggtgtttgcc agggtgacac
     1261 gtctcgttat ggacccgtta atattggtgc aaataccacc tttaccctgt atgtcaccaa
     1321 gccatttctc ggctcgatga ccattccgaa aacggatatt gccgtcatta aaggcgcgtg
     1381 ggtcgatgga atgggaagcc cgtctacagg tgacttccat gatttagtca agttatcgat
     1441 tcagggaaat ctcaccgccc cacagtcgtg caaaattaat cagggcgatg ttattaaagt
     1501 taattttgga ttcatcaatg gtcagaagtt taccacccgc aatgccatgc cagacggttt
     1561 tactccagta gactttgata tcacttatga ctgtggtgat acttcaaaga ttaaaaactc
     1621 gttgcaaatg cgcatcgacg gtacaactgg ggtagtagac cagtacaacc tggtcgccag
     1681 gcgaagaagt tcagacaatg tgcccgatgt cggtattcgt attgaaaatc tcggcggcgg
     1741 agttgcaaat attccttttc agaacggtat ccttcccgtt gatccttccg ggcatggcac
     1801 cgtcaatatg cgcgcctggc cagttaatct ggtcggtggt gagctggaaa caggaaaatt
     1861 tcagggcaca gccaccatta ccgtcatggt gcggtaaaag aagacgttgc gttttgatta
     1921 acgcaacgct tcggcactgt tacccctgct gctccagcgc atacttatac agcgcatttt
     1981 tcttcacgcc atgaatttct gcggccagcg ccgccgcttt tttcagcggc agttctgcct
     2041 gtagcagcgc cagcgtgcgc agggcatcgg cgggtaagtc ttcttcctgt gctttatgac
     2101 cttcgacaat cagcaccatt tcgcctttgc gacggttttc atcttccttt acccacgcca
     2161 gcagctcgcc aacgggcgcg ccgtgaatgg tttcccaggt tttggtcagc tcacgcgcca
     2221 gaaccacgta gcgggattcg cctaataccg caacgatatc ttccaggcta tctaacagac
     2281 ggtgggtaga ttcataaaaa atcagcgtgc gcggctccgc ttcaatggct tttagcgcat
     2341 cacggcggcc ttttgatttg gcaggtaaaa agccttcgta acagaaacgg tcagatggta
     2401 aacccgcggc gcttaacgca gtgatagcag cacacggtcc gggtagcggc accacacgaa
     2461 tccccgcttc acggcaggta cgcaccagat ggtagccagg atcgttaatt agcggcgttc
     2521 cggcatcgga aaccagcgca atgttttgcc cctcttgcag ctttgccagc agcgtttcgg
     2581 ctttttgttg ttcgttatgg tcgtgcagcg caaacaaccg ggcattaatc ccaaaatgtt
     2641 gcagcaataa accggtgtga cgagtatcct cggcggcaat cagatcaacg gcctgtaata
     2701 cctctaacgt acgctgggtg atatccgcca gattgccgat tggcgtcggt acaatgtaaa
     2761 gctggccctg agaattatcc gccgattggt gttgtttcat tgtgtcgtcc gtattgccga
     2821 tttaatattg agcattgcgt aaaaaaaata tcactggata cattatggta ccctcaacat
     2881 tttctcgttt gaaagccgcg cgttgtctgc ctgttgttct ggcagccctg attttcgccg
     2941 gttgtggcac ccatactccc gatcagtcca ctgcttatat gcagggcacg gcgcaggctg
     3001 attctgcctt ttatcttcag cagatgcagc aaagctctga tgataccagg atcaactggc
     3061 aattactcgc cattcgtgca ctggtgaaag aaggtaaaac cgggcaggcg gttgagttgt
     3121 ttaaccaact accgcaagaa ctgaacgatt ctcagcgtcg cgagaaaaca ctgctggcgg
     3181 cagagattaa actggcgcag aaagattttg ctggcgcgca aaacttgctg gcgaaaatca
     3241 cacctgccga tttagaacaa aaccagcaag cgcgttactg gcaggcaaaa atcgatgcca
     3301 gccaggggcg tccttccatt gatttactgc gcgcgttaat tgctcaggaa ccgctgcttg
     3361 gcgcgaaaga aaaacagcag aatattgatg ccacctggca ggcgctctcc tccatgactc
     3421 aggaacaggc gaatacgctg gtgatcaacg ccgacgaaaa tattctgcaa ggctggctgg
     3481 atctgcagcg cgtctggttt gataaccgta acgatcccga catgatgaaa gccgggatcg
     3541 ccgactggca gaaacgttat ccgaacaatc cgggcgcgaa aatgctgcca acgcagttgg
     3601 ttaacgtaaa agcgtttaaa ccagcctcga ccaacaaaat cgccctgctg ttgccgctga
     3661 atggccaggc agcggtattt ggtcgcacta ttcagcaagg ctttgaagcc gcgaaaaata
     3721 tcggcactca gccagtggcg gctcaggtag ctgccgcacc tgccgcagac gtagcagaac
     3781 aacctcagcc gcaaactgcg gatagcgttg ccagcccggc acaagcctcg gttagcgatc
     3841 tgaccggtga tcagcctgcg gcccagccgg tgcctgtaag cgccccggcg acaagcaccg
     3901 cagcggtaag cgcacccgca aatccatccg cagagctgaa aatctacgat acctcatcac
     3961 aaccacttag ccagatctta agccaggttc agcaggatgg cgcgagtatt gtggttggtc
     4021 cgttgctgaa aaataacgtt gaagagttgc tgaagagcaa caccccgctg aacgtactgg
     4081 cactgaacca gccggagaat atcgaaaacc gcgtcaatat ttgttacttc gcgctttcac
     4141 cggaagacga agcgcgcgat gcagcgcgtc atattcgtga ccagggtaaa caagcgccgc
     4201 tggtgctgat cccacgcagt gcattgggcg atcgcgtagc caatgcgttt gcgcaagagt
     4261 ggcagaaact gggcggcggc accgttctgc aacaaaaatt tggttccacc agcgaattac
     4321 gcgcgggtgt taacggcggt tctggtattg ctttaacggg tagcccgatt actcccagag
     4381 cgacaaccga ctccggcatg acgaccaaca atccaacgct gcaaaccacg ccaaccgatg
     4441 accagttcac caataatggc ggtcgtgtcg atgcggtcta cattgtggca acgccgggtg
     4501 aaatcgcttt tatcaaaccg atgatcgcca tgcgtaacgg tagccagagc ggtgcaacgc
     4561 tgtacgccag ttcccgcagc gcacaaggca ccgcaggccc ggatttccga ctggagatgg
     4621 aaggcttgca gtacagcgaa atcccgatgc tggcaggcgg taatctgccg ttaatgcagc
     4681 aggcgctcag cgcggtgaac aacgattatt cgctggctcg catgtatgcg atgggcgttg
     4741 atgcctggtc gttggcaaat catttctcac aaatgcgcca ggttcagggt tttgaaatca
     4801 acggtaatac cggaagtctg acggctaacc cggattgcgt gattaacagg aagttatcat
     4861 ggctacagta ccaacaaggt caggtagtcc ccgccagtta accaccaaac agaccggcga
     4921 tgcgtgggaa gcacaagcgc gtcgctggct ggaaggcaaa ggactgcggt ttatcgccgc
     4981 taacgtgaac gagcgtggcg gcgagatcga tctgataatg cgtgaaggcc tgaccaccgt
     5041 ttttgtcgag gtacgctatc gccgctctgc gctttatggc ggcgcggcag ccagtgtgac
     5101 ccgcagcaaa caacacaaat tattacagac tgcccgcttg tggctcgcgc gtcataatgg
     5161 gagttttgat actgtggatt gccggttcga tgtggtagcc ttcaccggga atgaggttga
     5221 gtggattaag gatgccttta atgaccactc ataattaagg tttaaggatt agcgtgcaag
     5281 aaagaattaa agcttgcttc actgaaagca ttcaaactca aattgcggcg gcagaggcgc
     5341 ttccggatgc catctcccgt gcagccatga cgctggttca gtctctgctc aatggcaaca
     5401 aaatcctctg ttgtggtaat ggaacttccg ctgccaatgc acagcatttt gctgccagca
     5461 tgatcaaccg tttcgaaacg gagcggccca gcttacctgc cattgcacta aatactgata
     5521 atgttgtctt aacggcgatt gccaacgatc gcttacatga tgaagtgtat gcaaaacagg
     5581 tgcgggcgct gggtcatgcg ggagatgtat tgttagccat ttccacccgt ggcaacagcc
     5641 gcgatattgt taaagcagtt gaagccgccg ttacgcgtga tatgaccatt gtggcattga
     5701 ccggctatga cggcggcgaa cttgcaggtt tgttagggcc acaggatgtg gagatccgca
     5761 ttccttcgca tcgtagtgct cgcattcagg aaatgcatat gctgacggta aattgcctgt
     5821 gcgatctgat cgataacacg cttttccctc accaggatga ttaaggagaa tacatgaagg
     5881 cattatcgcc aatcgcagtc cttatttccg cgctgctgtt gcaaggttgt gttgccgctg
     5941 ccgtagtggg taccgctgct gtgggtacca aagccgcaac tgacccacgc agtgtcggca
     6001 cccaggtgga cgatggtacc ctggaagtgc gcgtgaacag cgcattgtcg aaagacgaac
     6061 agattaagaa agaaacccgt attaatgtca cggcctatca gggcaaagtg ctgctggttg
     6121 ggcagtcacc aaatgctgaa ctttcggctc gcgccaaaca gattgctatg ggcgtagacg
     6181 gtgccaacga agtgtataac gagattcgtc agggccagcc gattggtctg ggcgaagcat
     6241 ctaacgatac gtggatcacc accaaagtgc gttcgcagct cttaaccagc gacctggtga
     6301 aatcgtccaa cgtgaaagtg accaccgaaa acggtgaagt gttcctgatg gggctggtga
     6361 ctgaacgtga agcgaaagcg gcggcagata ttgccagccg ggtgagcggc gtaaagcggg
     6421 ttaccacagc gtttacgttt attaaatagc agccccttgt aatgcctgat gcgacgcttg
     6481 ccgcgtctta tcaggcctac aacgatgcaa accgtaggtc ggataaggcg tttacgccgc
     6541 atccgacatg ttttccctca aatcagaaca acagcgccag cccgccgaca atcacaccag
     6601 acactgccac catcgcccct gtcagccata aggctttcgc cgggaacgct ttgcgcagca
     6661 taatcagtga cggcaaactc actgccggga gcgtcatcaa caatgccagc gccggagcgg
     6721 ttcccatacc tgccagcatc atcgtttgta caatcggaat ttctgctgcc gtgggaatga
     6781 caaacaagca tcctgctacc gccatcgcca ccacccacat caggctgtta tcgacagcac
     6841 catcggcatg ggggaataac cagacgcgtg cggcacccaa caccagtact gcaaggatgt
     6901 aaaccgggat cgtactccag aaaagtgtcc atagcgccct gccccagcgg ctaaaaaaac
     6961 cgctctgtgc ttccggtatg tcaatttcga ccggtgcctg cgtttgcggt gtttcacgca
     7021 cccatttttg caccagcgtc gcaatcagca acaccatcac cagcccggcc accagacgaa
     7081 tcgccgcaaa accccagccg aggacaaacc ccataaacac cagcgtcgcc gggtttaaca
     7141 ccggattgcc catccagaat gccagcgcac cgcccatcga tacctgttgg cgacgcattc
     7201 ccgccgcgac cggagccgca cagcaggtac acatcatgcc cggcagcgaa aacagcgttc
     7261 ccagcagcgt gccgcgaaag cgcgattgcc caagcgtacg caacaaccag tcacgcggga
     7321 tcagcacctg aatcaacgaa ccgaggatca cccccagcac cgccgctttc catactgcga
     7381 ggaaatagat catcgcgtaa tccaacgccg cttgccacgg attagcatcc gcctgcgcaa
     7441 ggatagattt accgatactg tgggtttcgg ctgcagtaaa cgctttgccg tagtaaggtt
     7501 gccatttcac ataccagaga ccggcaatga caacgagaaa gaaaagcgcg ggtttccacc
     7561 actgaatggg cgttgccgcc tgagatgaag actgaccagt catagcattc cccaggaata
     7621 agttatgata attgagcgcg tgaatattac gctcactatc aattcttggg aataattatt
     7681 cagctctttt gcgtaattct gaagagctta aaatcgtcac gccttcatgc tcaggccgca
     7741 tcgcctctgc cagcatgacc cgcgccacgt ctctcgcatc aatggatttc cagttacctg
     7801 gtaacaaacg gaacagcggc gcaaaaagcg tttcgttcat ccgctgtttg ctacgatcgc
     7861 ccagtaacat cgacgggcga gcaatggtca atttcggcca gttctgggca attaatgctt
     7921 cttccatctc ccctttgacg cggttataga aaaacggcga gtgggcattg gcacccatag
     7981 cactgaccac cagcatatgc tgcgcgccca gtcgccgccc ggttaatgcg gtatccacta
     8041 ccagcgtgta atcggcatga ataaacgcct ctttgctccc cgcttctcgc cgcgtggtgc
     8101 cgagacaaca aaacacaatg tcgatgggtt cggtgacctg cgccaacgcg tcactcagtt
     8161 gcggatcatg gggattaaac accccgggca tatcgcccaa cggacgtcgc gtcggcgcag
     8221 caatggcgtt aactttcggt tcattaatca acatccgcag caggtgaccg cccaccagcc
     8281 ccgttgcgcc tgtaatcagt acctgactca tcttcgttcc tttacagaat tgtctgcctt
     8341 gcgctccccg gctcatgcac cagtcttaat agaccatgag gtaattatcc ccgattgtgg
     8401 aaaattcgcc tcatccaatg caacaacgcg gaggaagcat gagtaagaaa attgccgttt
     8461 taatcactga tgaatttgaa gattcagaat ttacttcacc cgcagacgag ttccgtaaag
     8521 ccggacacga agtgattact attgaaaaac aagcgggtaa aacggtgaaa ggcaaaaaag
     8581 gagaagccag cgtgaccatc gataaatcca tcgatgaagt gacgccagcg gagtttgatg
     8641 ccctgttgct accgggtggt cattcaccgg attatctgcg tggcgacaac cgttttgtca
     8701 cctttacccg tgattttgtg aatagcggca aacctgtttt cgccatctgt cacggtccgc
     8761 agctgctgat cagcgccgat gtgattcggg ggcgcaaact gaccgcagtt aaaccgatca
     8821 ttattgatgt taaaaatgcg ggcgtggaat tttacgatca ggaagtcgtg gttgataaag
     8881 atcagctggt taccagccgg acaccggacg atctgccagc gtttaaccgc gaggcgttac
     8941 gcctgctcgg tgcctgagtc gcgtagccaa atcatttttt taccaaagcc cagcgtgttg
     9001 tcggtgaatt tgatttcatc gagccggatt tcccacaccg gggccgacag cattctggca
     9061 accggaaagc gacgattgta cgccttgcgc gcgaggtcgc tttcctcacc ttccagcctg
     9121 cggatctcac ctttaaactg cacaccgcga attaacgcta ccgttttcgg ctgaccgttt
     9181 accgttccgg caaccgcagc ctgcggccca ctcatctgcg cgtggcgcgt tttttcttcc
     9241 gtcaaaatgt agaaggcgac tttctgcgca tcaaaaagat aaaaggcatt agcgcaccag
     9301 agttcccctt cctgctgcac acaccaggtg acaacatgtt gttttgccag ccaacggctg
     9361 atggcgatga gtgtttccat tgctgttctc ttttatactg tgggcctgac cttaacatgc
     9421 gatgcagacg atgacaccct ggtttcttta cttgatccgt accgccgaca ataagcttta
     9481 taccgggatc accacggatg tcgaacgccg ctatcagcag caccaaagcg gcaaaggggc
     9541 gaaagcactg cgcgggaaag gagaactgac gctggcgttt tctgcgccag tgggcgaccg
     9601 ttcgctggca ctacgggcgg aatatcgcgt taagcaactg accaaacggc aaaaagagcg
     9661 tctggtagcg gaaggcgcag ggtttgcaga actgttgagc agcctgcaaa ccccggagat
     9721 taaaagcgat tgaaatgctc gtgatactca accagaccgg ttacgccatt cagcgcgtca
     9781 tccgctaaac gatgtaccag gaaggcgctt tcagtgcccg gccagcggca acgcagatcg
     9841 tgatgcgccg ccagttcaaa gccgaaacga ctgtacagcg ccggatcgcc cagcgtcacc
     9901 actgcggcat agccgaactc attaagcgaa tcgagtcctt catagaccag ttggcgtgcc
     9961 agcccctgtc cacggtattt ttcatcaacc gccaacggtg ccatgccgac ccattgcagg
    10021 tcttcgccct gcacatcaac cggactaaat gccacatagc caatgacctg accttcgtca
    10081 tctgtcgcca ccagccccag cgtcagaaag ccatcttcac gcagatcgtg aaccagcttc
    10141 gcttccgcat cactttcgaa tgagcgacgc agcagggcat caatacccgg cgcatcaatg
    10201 ggaatttcta ctcgaattag catggttcac ctacagatgt ctgtttggtt tcaggcgcgg
    10261 ttttcattcc cgcctcaaca aaatccgcca gttgcagcag catcatgcgt aaggctttcg
    10321 gcatttgctc cagttcaatg gcgtccatca ggtttttcac atacaccccc agctccgtat
    10381 cgccttcaat caccagccga cgctggaaga agagcgtatc cggatcttgt ttacgcgccg
    10441 caatcatcag cagatcgctg gcgtcggcac taaaactcac atcagcttgc gtgttctggc
    10501 tgaccaccag tttgccattc accaccgagg taaaccattg caggtcaata tcacgcacat
    10561 gaatacttaa ccagcggcct tcaagaaact ccagctcgcc atcatccagc gcctgacgga
    10621 attgccagct taagacctgc tcaagaacct ggcgttttag cgcaaatggc gtcagtttta
    10681 ccggtacact caacagagat ggccccaaat gcacaatacg ggaacgcagt ttatccaaca
    10741 cgagttttac tccctgtttc aacaatcatc ctattttgcc atatcagaaa aataacatag
    10801 cggtataaat caacaattcc atatgaaatt gctgctacca ccaatacaac tttaactgcc
    10861 ttaaatcaaa aattgtcgca gcaaggttaa ctaaaatccc agttcgttaa catttttgcg
    10921 ttttgatagc gcaaccttca ggaaaaatta tggagctgct ctgccctgcc ggaaatctcc
    10981 cggcgcttaa ggcggccatc gaaaacggcg cagatgctgt ttatatcggg ctaaaagatg
    11041 ataccaatgc ccgtcacttc gccggcctta actttaccga gaaaaaattg caggaagcgg
    11101 tgagttttgt ccatcaacat cgccgcaaac ttcacatcgc gattaacact tttgcgcatc
    11161 cggacggtta cgcccgttgg cagcgtgccg tggatatggc ggcgcagctg ggtgccgacg
    11221 cgctgatcct cgccgacctc gccatgctgg agtacgccgc cgagcgttac ccgcatattg
    11281 aacgtcatgt gtcagtgcag gcttcggcga ccaatgaaga ggcgattaac ttttatcatc
    11341 gccattttga cgttgcccgc gtggtgctgc cgcgcgtgtt gtcgattcat caggtgaaac
    11401 agctggcacg ggttactcct gtcccgctgg aagtgtttgc tttcggcagc ctgtgtatta
    11461 tgtcggaagg ccgttgctat ctgtcgtcgt atctgacggg tgagtcgccc aacactgtgg
    11521 gtgcgtgttc tccggcccgt ttcgtgcgct ggcaacaaac gccgcagggg ctggaatccc
    11581 gcctgaacga agtgctgatc gaccgttatc aggacggcga aaacgcaggt tatccgacgc
    11641 tgtgtaaagg gcgttatctg gtggacggcg agcgctatca cgcgctggaa gaaccaacca
    11701 gtctcaatac cctggaactg ctgccggagt taatggctgc gaatattgct tcggtgaaaa
    11761 ttgaaggccg ccagcgtagc ccggcgtatg tcagccaggc ggcgaaagtc tggcgtcagg
    11821 ctatcgaccg ttgtaaggcc gatccgcaaa acttcgtacc gcaaagcgcg tggatggaga
    11881 cgctcgggtc gatgtccgaa ggcacgcaaa ccacccttgg cgcgtatcac cgtaaatggc
    11941 agtgagaaga gcaatgaaat attccttagg gccagtgctg tggtactggc caaaagagac
    12001 gctggaagat ttttatcagc aagccgccgc cagcagcgcc gacgtgattt atcttggtga
    12061 agcggtatgc agcaagcgcc gggcaaccaa agttggcgac tgtctggaga tggcaaaatc
    12121 gctcgccgga agtggcaagc agattgtgct ctccacgctg gcgctggtgc aggcatcctc
    12181 tgaactgggc gaactgaaac gctatgttga gaacggtgaa tttctgattg aagccagcga
    12241 tctcggcgtg gtgaatatgt gcgctgaacg caaactgccg ttcgtcgccg ggcacgcgct
    12301 gaactgctac aacgcggtga cgctgaaaat attgctcaaa cagggcatga tgcgctggtg
    12361 tatgccggtg gagctttccc gcgactggct ggtgaatctg cttaatcagt gcgatgagct
    12421 gggcattcgc aaccagtttg aagtggaagt cctgagctac ggtcatctac cgctggccta
    12481 ctccgcccgc tgctttaccg cgcgttcgga agaccgcccg aaagatgagt gtgaaacctg
    12541 ctgcattaag tatccgaacg ggcgcaacgt gctgtcgcag gaaaaccaac aagtgtttgt
    12601 actcaatggc attcagacca tgagcggcta cgtttacaac ctcggtaacg agctggcatc
    12661 catgcaggga ttggttgatg tagtccgcct gtcaccgcag ggcactgaca ctttcgcgat
    12721 gctcgacgcc ttccgcgcta atgaaaatgg cgctgtgcca ctgccgttga cggcaaacag
    12781 cgactgtaac ggctactggc ggcggctggc aggactggag ctgcaagcct aagtaaatag
    12841 ctcactttgt taacaacttt aactattctt taatgcagta ttaaagatta atcggtaaca
    12901 aagtgagctg ttatgactga taaaaccatt gcgttttcgc tactcgatct ggcccccata
    12961 cccgaaggct cttcagcgcg ggaagcgttc tcccactctc tcgatctcgc ccgtctggct
    13021 gaaaagcgcg gctatcatcg ctactggctg gcagaacacc acaatatgac cggcattgcc
    13081 agtgctgcaa catcggtatt gattggctat ctggcggcga ataccaccac gctgcatctg
    13141 gggtctggcg gcgtgatgtt gcctaaccac ttaccgttgg tcattgccga acagttcggc
    13201 acactcaata cgctctatcc ggggcgaatc gatttggggc tgggtcgtgc tccgggtagt
    13261 gaccaacgga caatgatggc gctacgtcgt catatgagcg gcgatattga taatttcccc
    13321 cgcgatgtcg cggagctggt ggactggttt gacgcccgcg atcccaatcc gcatgtgcgc
    13381 ccggtaccag gctatggcga gaaaatcccc gtgtggttgt taggctccag cctttacagt
    13441 gcacaactgg cggcgcagct tggtctgcca tttgcgtttg cctcacactt cgcgccggat
    13501 atgttgttcc aggcgctgca tctttatcgc agtaacttca aaccgtcggc tcggctggaa
    13561 aaaccatacg cgatggtgtg catcaatatt atcgccgccg acagcaaccg cgacgctgaa
    13621 tttctgttta cctcaatgca gcaagccttt gtgaagctgc gccgtggcga aaccgggcaa
    13681 ctgccgccgc cgattcaaaa tatggatcag ttctggtcac cgtctgagca gtatgacgtg
    13741 cagcaggcgc tgagtatgtc gttggtaggc gataaagcga aagtgcgtca tggtttgcag
    13801 tcgatcctgc gcgaaaccga cgctgatgag attatggtta acgggcagat tttcgaccac
    13861 caggcgcgcc tgcattcgtt tgagctggcg atggatgtta aggaagagtt gttgggatag
    13921 tgtgtcttaa cgcgggaagc cttatccgag ctggcaacgc tgtcctacgt aggcctgata
    13981 agcgaagcgc atcaggcatt gtgtaggcag cagaaatgtc ggataaggca ccgctgatta
    14041 ctgatacacc ggcagtaaat taaagctcga taaaatatgc accagtgcgt tgccgacgcc
    14101 aaacaccaga atcagcgcaa tcatcggctt gccaccccag acgcggaatt tcgggctgcc
    14161 aaagcgttta cgcgatgcgc gggccagcag tgccggaaca attgccgccc agatggtagc
    14221 cgctaaacca gcataaccaa tggcatacag gaatccgttc gggaacagca gaccacccac
    14281 aacaggcggg gcaaaggtca gcaatgccgt tttcaagcgg cccacagccg agtcgtcgaa
    14341 accaaacaga tctgccagat agtcaaacaa acccagcgtt acgccaagga acgaactcgc
    14401 taccgcaaag tttgagaaca cgaccagcag aaggtccagg ctacggctgt tcagcacgcc
    14461 gcttaatgct tgtaccaaca catcaatatt accgcccttc tcggcgatgc cgataaactc
    14521 cggacgaggg atgttgccca ttgtcgccag caaccagatg gtatacagcg ccagtgccat
    14581 cagcgtaccg tacaccaggc atttcacgat ggttttcgga tctttgccgt aatacttcat
    14641 caggcttggc acgttaccgt gataaccaaa cgacgccagg cagaacggca gggtcatcag
    14701 caaatacggc gcataagatg cattgctttc ggcaacgttg aacaatgtcg caggctgcac
    14761 atgccccagc aggctaccga aggtgaggaa gaaggtaatg actttcgccc ccagcacaat
    14821 cgccgtcatg cggctgacgg ctttagtact caaccacacc acaaacgcta ccagcagtgc
    14881 aaaaccaaaa cccgccgccc gtgccgggac gtttagcgac atctctgcga aggtgtgatg
    14941 cagaatcgaa ccactggcag aaatataggc ataggtcagg atatagagca caaaggcaat
    15001 ggaaatgccg ttgaccacgt tccagccttt gcccagcaaa tctttggtga tggtgtcaaa
    15061 actcgaaccg attcggtaat tcaggttagc ttccagaatc atcaagccgg aatgcagcat
    15121 acagaaccag gtaaagatca gcgctgccat tgaccagaaa aaccacgccc cggacatgac
    15181 cactggcaga gaaaacatcc ctgcgccaat aatggtgccg ccgataatca ccacgccgcc
    15241 aagcagcgac ggtgacgttt gggtggtggt tagtgttgcc atgagggctt ctctccagtg
    15301 aaaaatagtg cgactgcgtt gttatgcatt gcactgtacc agtacatgag tacaaaagac
    15361 agaaaaaaag ccccgatggt taaaatcggg gctgtatata ttattttaca gattgtgttc
    15421 gctgttcagc gatgattacg catcaccacc gaaacgacga cgaccggtag agtcatcacg
    15481 acgcggtgcg cggccttcac gacgttcgcc gctaaaacga cgaccatcac cacgaccacc
    15541 ttcacggcgt tcaccgctga agttacgacc gccttcacga cgctcgccac cgaaaccacg
    15601 accaccgcca cgacgctcac cgccagtatg cggctgtgca tcgcccagta actgcatgtt
    15661 catcggcttg ttgagaatgc gagtgcgcgt aaagtgttgc agcacttcac ccggcatacc
    15721 tttcggcagt tcgatggtgg agtgagaagc aaacagcttg atgttaccga tgtaacggct
    15781 gctgatgtcg ccttcgttag caatcgcgcc aacgatatga cgaacttcaa caccatcatc
    15841 gcggcccact tcaatgcggt acagctgcat atcgccaaca tcacgacgtt cacgacgcgg
    15901 acgatcttca cggtcgccac gcgggccacg gtcgttacga tcgcgcggac cacggtcatc
    15961 acggtcacgg aattcacgtt tcggacgcat cggcgcatct ggcggaacaa tcagagtacg
    16021 ttcaccctgt gccattttca gcagtgccgc agccagagtt tcgagatcca gctcttcacc
    16081 ttctgcagtc ggctgaattt tgctcagcag tgcgcggtat tgatccagat cgctgctttc
    16141 cagctgctgc tgtactttag cggcgaattt ttccagacgg cgtttgccca gcagttctgc
    16201 gttcggcagt tctacttccg gaatagtcag cttcatagta cgttcaatgt tgcgcagcag
    16261 acgacgctcg cggttctcaa cgaacagcag cgcgcggcca gcacgacccg cacgaccggt
    16321 acgaccgata cggtgaacgt aagactcaga atccatcggg atatcgtagt taaccaccag
    16381 gctgatacgc tcaacgtcca ggccacgggc cgcaacgtcg gtcgcaatca ggatgtccag
    16441 acgaccgtct ttaaggcgtt ccagtgtctg ctcacgcagc gcctggttca tgtcaccgtt
    16501 cagcgcggcg ctattgtagc cgttacgctc aagagcttca gccacttcca gagtcgcgtt
    16561 tttggtacga acgaagataa tcgccgcatc aaaatcttcc gcttccagga aacgtaccag
    16621 tgcttcgttt ttacgcatac cccacacagt ccagtagctc tggctgatgt caggacgggt
    16681 agtcacgctg gactgaatac gcacttcctg cggctctttc ataaagcggc gggtaatgcg
    16741 acgaatcgct tccggcatgg ttgcagagaa cagagcggtc tgatgacctt ccgggatctg
    16801 cgccataatg gtttcaacgt cttcgatgaa gcccatgcgc agcatttcgt cagcttcatc
    16861 cagaaccaga ccgctcagtt tagagaggtc cagagtgcca cgtttcaggt ggtccagcag
    16921 acggcccgga gtaccgacaa cgatctgcgg cccctgacgc agggcgcgta attgcacgtc
    16981 ataacgctgg ccgccgtaca gggcaaccac atttacgccg cgcatgtgtt tagagaaatc
    17041 cgtcattgct tcagcaacct gtaccgccag ttcgcgggtc ggtgccagca ccagaatctg
    17101 tggtgctttc agctcaggat caagattctg cagcagaggt aaagagaatg ctgcagtttt
    17161 tccgctcccc gtctgggcca tacccagaac gtcgcggcca ttcagcagat gtggaataca
    17221 ctctgcctga attggagatg gtttttcgta acccagatcg ttaagggctt caaggatagg
    17281 agccttcagg cccagatctg caaaagtggt ttcgaattca gccatgtagt acgtgtgcct
    17341 caaaattaat ggcggccagt ctacataact catcatgaaa ttgatcagca attttcattg
    17401 aaaagtgtga accggctcaa agtaggtgta ttaacgaaca acaacgccct cacccgttaa
    17461 ggtgatggca atcaaaaaag attacgggct gatgtgtacg tcagctattg ctggtccgat
    17521 tctgccaggt catcttggtc ctggcccagg agcgataatt ccaacaatgc gtatcggtgc
    17581 tcaacaaagt tatgaacgtt gttggcaacc gccagtttga acagtgccgt ggcgctgtcc
    17641 aaatccccca gacttaggta gtacttacct aaatagaagt tggtttcact gagatgctca
    17701 gcgagcgagg tgttatccgt tgcgtccgcc ttgagccttt ccattaacgt ttgttcgcta
    17761 atgttgccca ggtagaactc gacaatgttc catccccact gttccttatc cgatttttcg
    17821 aagtgctgtt tcaatacttc tttagcctgc ttctcatcga gcttctgctc ggcgagataa
    17881 agccacagac tacggaaagg atcattggga tcgtcttgat aaaacgccag cagatcatct
    17941 tgcgctaact tgtcacgacc gccgtaatat aatgcaatcc cgcgattcaa gtgcgcgtag
    18001 ttgtaagttg gatcaagctc aagtacagaa tcaaacgctt cataggcagc atcaaaattg
    18061 cctgcctgcg ttaaatatat gcctaagtaa ttgaatactt caggcatatc cggtcggatt
    18121 gccagcgctt gcgaaaaatc gttacgcgct aatgccctca gaccgagact atcatacaac
    18181 actccgcgct catataaaag ctgtgcgcgt tcgtcatcgg ttaaagcccg actggcaagg
    18241 atttgttcca tacgtgccag aatcacttcc tgctgtaaag tcggttgcaa tggtaccgcg
    18301 aggacttcac ttttacgcca ggaagtatta ctgcatcctg caagcgtaag tgctgtcgca
    18361 acgaaacacc agcgcaaaaa aggcttcatt tcccactccc gaagaccacg gttgaatgaa
    18421 cgtcctgttc ccggttgcta acaaggcgtc ctgcccggtt aaaagccccc cgccgcagcg
    18481 gagggcaaat ggcaacctta ctcgccctgt tcagcagccg gagcttccgg tgctgcagca
    18541 ggttgagact gctcagtcgc ttctttaatg ctcagacgga tacggccctg gcgatcaact
    18601 tccagaactt tcaccggtac ttcctgaccc atctgcaggt aatcggtcac tttctcaacg
    18661 cgtttgtcag cgatttgaga gatgtgtacc agaccttctt taccgccgcc gatggcaaca
    18721 aatgcgccaa agtcaacgat acgggtcact ttaccagtgt agacgcggcc cacttcgatt
    18781 tctgcagtga tctcttcgat acgacgaata gcatgtttcg ctttctcgcc gtcggtcgct
    18841 gcaatcttca cagtaccgtc atcttcgatt tcgatggtgg tgccagtttc ttcggtcagg
    18901 gcacggataa cagagccgcc tttaccgata acgtctttga tcttgtccgg gttgatcttg
    18961 atggtatgga tacgtggtgc gaactcagag atatcgccac gcggcgcgtt gatcgcctgt
    19021 tccattacgc ccaggatatg cagacgcgca cctttagcct ggttcagcgc aacctgcatg
    19081 atctctttgg tgataccttc aattttgata tccatctgca atgcagagat accctcgcgg
    19141 gaacccgcaa ctttgaagtc catatcgccc aggtgatctt cgtcgcccaa aatgtcagac
    19201 agtacaacgt agttgtcgcc ttctttcacc agacccattg cgatacccgc aacggcagct
    19261 ttgatcggca cacctgcgtc catcagcgcc agagacgcgc cgcacacgga agccatagaa
    19321 gaggaaccgt tggattcagt gatttcagac acaacacgta cggtgtacgg gaatttgtcc
    19381 atatccggca tgactgccag cacgccgcgc ttcgccagac gaccgtgacc aatttcacga
    19441 cgcttcggag aaccgaccat gccggtttcg cctacggagt acggagggaa gttgtagtgg
    19501 aacaggaagg tatcggtacg ttcgcccatc agttcatcaa gaacctgcgc gtcacgagca
    19561 gtacccagcg ttgcggtaac cagcgcctgc gtttcaccac gggtgaacag cgcagaaccg
    19621 tgagtacgcg gcagcacgcc agtacgcaca tccagaccac ggatcatatc tttttcacga
    19681 ccgtcgatac gcggttcgcc tgccagtacg cggctacgaa caacgttttt ctcgatcgcg
    19741 tgcagaattt cacccagttc gttttcgtcc agggtttcgt cttcagcaag cagcgtcgcg
    19801 atggtttcag atttgatgac atcaacctgc gcataacgct cttgtttgtc ggtgatgcgg
    19861 taagcatcgc tcaggcgagc ttcagccagt gcagcaacgc gcgcgttcag cgcttcgttt
    19921 accggctccg gctgccagtc ccaacgtggt ttaccggctt ctttcaccag ttcattgatg
    19981 ttctgaataa caacctgctg ttgttcatga ccgaacacta ctgcgcccag catctggtct
    20041 tcgctcagca gttcagcttc agattcaacc atcagtacag cggcttcagt accggcaaca
    20101 accagatcca gtttgctctc tttcaactcg tcctgagtcg ggttcagtac gtactggtca
    20161 ttgatgtaac ctacgcgagc agcaccaatc ggaccattga acggaatacc agacagagac
    20221 agtgctgcgg aagcaccaat catcgcgacg atatccgggt taacttgcgg gttaacagaa
    20281 accacggtgg cgataacctg aacttcgttg acgaagcctt ccgggaacag cggccgaatc
    20341 gggcggtcaa tcagacgcgc gatcagggtt tcgccttcgc ttgggcggcc ttcacgacgg
    20401 aagaagctac ccgggatacg accagcagcg taggtacgct cctgatagtt aacggtcagt
    20461 gggaagaagt cctgacctgg tttggctttt ttctggccaa caacggtaac aaataccgcg
    20521 gtgtcatcca tgctaaccat aacagcggca gtagcctgac gagccatcat gccggtttcc
    20581 agagtcacgg tgtgttggcc gtactggaat ttacgaacga tcggattaag caatgtaata
    20641 tcctttctct ttcttagaca gtaccttacg gcactggtgt taatacccga tcttctgcgc
    20701 atcctcgcga ctaatgacaa ccctaaccca gctctatgtg ggtaaagcct ctcattagcc
    20761 gcgcgaacct ctgcaacgga agatcattca tagcaacaat acattagttt ccagtgaatt
    20821 gctgccgtca gcttgaaaaa aggggccact caggccccct tttctgaaac tcgcaagaat
    20881 tagcgacgca gacccaggcg ctcgatgagc tgggtgtaac gtgctacgtc tttacgtttc
    20941 aggtagtcga gcagtttacg acgctgagaa accatgcgca gcagaccacg acggctgtgg
    21001 tgatcttttt tgtgctctgc aaagtggccc tgcaggtggt tgatctgtgc agtcagcagt
    21061 gctacctgaa cttcagtaga accggtgtcg tttgcgtcac gaccaaactc agaaacgatt
    21121 ttagctgttg cttcagtact tagagacatt ttaaaactcc aaagtatata gaatgaaagg
    21181 acgccgatct ctaattcagc gatcccagtg tacgttacgc aaagtgttaa acaatttacg
    21241 cgacgttaag cggcagtatt ctactcgtag cgacctgtta tcgcaagacg gttaacatta
    21301 cgccgggtat tcaaccacca ggcgacgagg cgcaacgcgg ccttcatcgt caatttcgcc
    21361 cataccgata aatttgccgt tctcaccttc tgtaacgcga accagtcctt ccagcggcgc
    21421 accggatgta cgaactgggt taccattttt gaagtaaaca gaagacgtta acggaagatt
    21481 caccaccggg tagtccgaag ctggactgtc cattggcatc agtaatggat caagtaactc
    21541 cgcagccgga atatcctgct gttcggcttg ttcgaccagt tcacgcagat gttccagggt
    21601 cacaatccgt tcaaccggat atttacttac cgccagacga cgcaggtaaa taacatgcgc
    21661 gccacaaccg agtttttcac ccaggtcatc aatgatggtg cggatataag tgccttttga
    21721 gcagtgaatt tccagctcca gctcattgcc ttcgtggcga ataaacagca attcataaac
    21781 ggtaatcggg cgcgcttcac gcggtacttc aatgccctga cgcgcatatt cgtacagttt
    21841 tttaccctga tatttcagtg ctgaatacat cgaagggatc tgttcgatat cgccacggaa
    21901 agtatccagc gccgccgcca gttgctctgc gctaaaggtt accggacgtt cttcaacgat
    21961 ttgtccgtcg gcatcagaag tatcggtacg ctgtccaaga cgcgcaatga cccgatagcg
    22021 tttgtcggag tccagcagat actgggaaaa cttcgtcgct tccccgaggc aaattggcaa
    22081 catgccagtt gccagcgggt ccagcgcacc ggtatgcccg gcacggttgg cgttataaat
    22141 ccgtttcact ttttgcagcg catcattgct ggacataccc tgcggtttat ccagcaacaa
    22201 aacgccgtta atgtcgcgac cgcgacgacg aggacgactc attagtcctc cttgctgtcg
    22261 tccgggttaa cacgacgttc ttcgtcatgt ttgaccacgc tggtcaccag gtttgacatg
    22321 cgcatccctt caaccagaga gttgtcgtag aagaaggtca gttccggcac gatacgcagg
    22381 cgcatcgctt tccccagcag gctgcggatg aaaccagaag cttcttgcaa cgctttgatg
    22441 cccgctttaa ccgcgtcttc atctttgtcg ttgaggaacg tcacatatac tttggcatac
    22501 gccaggtcgc gagacatttc gacaccggaa acggtggtca tcatgcccag gcgaggatct
    22561 ttaatttcac gctgcaggat gagagcgatc tctttttgca tttcctgcgc tacgcgctgc
    22621 gggcgaccaa attctttcgc cataataaat tctcctgaca aaaaaggggc tgttagcccc
    22681 tttttaaaat taatttcagg tggaagggct gttcacgttg gcctgataag acgcgccagc
    22741 gtcacatcag gcaatccatg ccggatgcgg cgtaaacgcc ttatcccgca tggaacccta
    22801 aaaaccttaa gcgatggtac gttggatctc gatgatttcg aatacttcga tcacatcgcc
    22861 agtgcggacg tcgttgtagt tcttaacgcc gataccacat tccataccgt tacggacttc
    22921 gttaacgtca tctttgaagc ggcgcaggga ctccagctcg ccttcgtaga taaccacgtt
    22981 gtcacgcaga acgcggatcg ggttgtgacg tttaaccaca ccttcggtaa ccatacagcc
    23041 tgcgatggca ccaaatttcg gcgatttgaa cacgtcacga acttccgcca gaccgataat
    23101 ctgctgtttc agttccggag acagcatacc gctcatcgcc gctttcactt cgtcaatcag
    23161 gttatagatg acggagtagt aacgcagatc caggctttcc gcttcaatca ctttacgtgc
    23221 agaggcatca gcacgtacgt tgaagccaac caggatggcg ttggacgccg cagccagggt
    23281 ggcgtcggtt tcggtgatac cacctacgcc agaaccgatg atcttcactt taacttcgtc
    23341 agtagacagt ttcagcaagg agtcggagat cgcttccaca gaaccctgta cgtctgcctt
    23401 caggacgatg ttcacttcgt gaacttcgcc ttcggtcatg ttggcgaaca tgttctcgag
    23461 tttagatttc tgctgacgcg ccagtttaac ttcgcggaat ttaccctgac gatacagtgc
    23521 aacttcacgc gctttcttct cgtcacgtac aacggtaact tcatcacccg cagccggtac
    23581 gccggacagg ccgaggattt ccaccggaat ggacggaccc gcttccagca cttcctgacc
    23641 cagttcgtta cgcatcgcac gaacacgacc gtattcgaag ccacacagaa cgatatcgcc
    23701 cttgtgcaga gtaccttcac gtaccagaac ggtagcaacc ggaccacgac ctttatcgag
    23761 gaaggattcg ataaccgcac cgctcgccat acctttacgt accgctttca gctccagaac
    23821 ttccgcctgc agcaggatag cgtccagcag ttcatcgata ccagtacccg ctttcgcaga
    23881 cacatgtacg aactggcttt caccgcccca ctcttccggc aggatgccgt actgggagag
    23941 ttcgttctta acgcgatccg gatcggcttc tggtttatcg atcttgttca ctgcaacgac
    24001 caccggaacc tgcgccgctt tcgcgtgctg gattgcttcg atcgtctgcg gcattacgcc
    24061 gtcgtcggca gcaacaacca ggactacgat gtccgttgcc tgcgcaccac gagcacgcat
    24121 tgaagtaaac gcggcgtgcc ccggggtgtc caggaaggtg atcatgccgt tttcagtttc
    24181 aacgtggtat gcaccaatgt gctgggtaat gccgcccgct tcgccagagg ccactttcgt
    24241 tgaacgaatg tagtccagca gagaggtttt accgtggtca acgtgaccca tgatggtcac
    24301 aaccggcgcg cgcggttcag ccgcagcacc cgtgtcacgg tcgctcatta ccgcctcttc
    24361 cagctcgttt tcacgacgca ggataacttt atggcccatc tcttcagcaa ccagctgtgc
    24421 ggtttcctga tcgataacct ggttgatggt tgccattgcg cccagtttca tcatcgcttt
    24481 gatgacctga gagcctttaa ccgccatctt gttcgccagt tcgccaacgg tgatagtttc
    24541 gccgatcaca acgtcacggt taacggcctg agcaggcttc tggaagcctt gctgcagcga
    24601 agaaccttta cgttttccgc ctttaccgcc acgtactgct gcgcgtgctt cttcacgatc
    24661 agcttttgat tcagcgtgtt tgttgccttt cttcggacgc gctgctttcg cgttacgacc
    24721 acggccacgg ccgccttcga cttcacgatc gctttcgtct tctgcctggc gagcatgttg
    24781 agaagtagtg acgtgataat cgctggaatc ttcagtcggt tccgcgttat cagtccattt
    24841 gttttcttcc gccatacgac gtgcttcttc agcaacgcga cgtgcttctt cttcgagttt
    24901 acgacgcgct tcttcttcag ctttacgctt gagctctgca gcttcctgct cacggcgggc
    24961 tttttcagcc tgggcgtttt tagtcatatc gtcttgttga ttgctcactt tgtctttttc
    25021 cgcagcttca cgtttcgctt gttcagcagc ttcacgctta gcttgttctg cggcctcacg
    25081 ttcagctttt tgttgcgcct cgcgtttagc cgattcttct gcctcacgac gggcttgctc
    25141 ttccgctgca aggcgttcag cctcttgcgg atcgcgtttc acaaaggtgc gtttcttgcg
    25201 gacttcgatt tgtaccgatt tgctttttcc accggtacca ggaatgttaa gggtgctgcg
    25261 tgttttacgt tgcagcgtca atttgtccgg gcctgaattt ttctgattca ggtggtcaat
    25321 caaagtctgt ttctcttgtg cagacacaga gtcgtcagca gacttccgga tacctgcatc
    25381 agcaaattgc tgtaccaggc gttccacgga ggtctgtcgc tctgcggcca gcgttttaat
    25441 cgttacatct gtcatgctgt tccttcctgc tacagtttat tacgcttcgt caccgaacca
    25501 gcaaatatta cgggcagcca taatcagtgc tccggctttt tcgtcggtca acccttcgat
    25561 atcagccaga tcatcaatgc cctgttcggc gagatcttcc agcgtacaaa cgccacgggc
    25621 ggccagtttg aatgccaaat cacgatctac cccttcaagg ttcagcagat cgtcagccgg
    25681 tttgttatca ccgaggcttt cttcctgggc ctgtgcaatg gtggccagtg catttttagc
    25741 acgctcgcgc agtgcttcaa cggtcggctc atcaaggcct tcgatttcca acagctcttt
    25801 catcggcaca taggccagtt cttccagcgt cgagaaacct tcttctacca gaacagtcgc
    25861 gaagtcttcg tcgatgtcga gatatttggt gaaggtgtcg atcgctgcgt gcgcttccgc
    25921 ctgatgctta gcctgcaggt cgtcaacggt cattacgttg agttcccaac cgctcagctg
    25981 cgaagccaga cgcacgttct gaccgttacg gccaatcgcc tgcgccaggt taccagcttc
    26041 aacggcgata tccatggtgt gtttatcttc atccaccacg atagaagcaa cgtctgccgg
    26101 tgccattgcg ttaatcacaa actgcgccgg gttatcatcc cacaggacga tatcgatacg
    26161 ctcgccgccc agttcagcag aaaccgcctg aacacgcgcg ccacgcatac ctacgcaagc
    26221 acctaccgga tcgatacgtt tatcgttggt tttcaccgcg attttcgcac gagaacccgg
    26281 gtcgcgagcc gctgctttaa tttcaatcac ttcttcgccg atttccggca cttcaatacg
    26341 gaacagttcg atcagcattt ccggcttgga acgagtgacg aacagttgcg cgccacgcgc
    26401 ttccgggcga acggaataaa gcacgccacg aacgcggtcg ccagggcgga agttttcacg
    26461 cggcagcata tcttcgcgca ggatcacggc ttcagcgttg ttgcccagat ccagagagat
    26521 gttgtcgcgg tttacttttt tcaccacgcc ggtgatgatt tcaccttcgt gttcacggaa
    26581 ctgatcaacc accatcgcac gttcggcttc acgcactttc tgcacgataa cctgttttgc
    26641 cgtctgggtg gtgatacggt caaaggtaac agactcaatc tgatcttcaa cgtaatcgcc
    26701 caagttcagg ctttcatctt cataacgtgc ggcttcaagg gtgatttcct tggtcggctg
    26761 ggtgacttca tcaacaacta accagcgacg gaaggtgtca aaatcaccgc ttttgcgatc
    26821 gatctgtaca cggacgtcga tctcttgctc atattttttc tttgttgctg tcgccagcgc
    26881 gctttccaat gcttcgaaaa tcttctcgcg aggtagcgcc ttttcattgg atacggcttc
    26941 aactacagcc aaaatttctt tgttcatcgc gggcttttca cctcatccag actattaaaa
    27001 gtggggaacc aggttcgcct tctggatatt actcagcgcg aacacttcat ctttaccttc
    27061 gacggtaact gtgatcattt caccgtctac cgctttgata acgccctgcc atttacgacg
    27121 gttttgtacc gccatacgga gaaccagagt cacctcttct ccgacaaaac gggcgtagtg
    27181 ttcagccgtg aacagtgggc gatcgagacc cggtgaggag acttccaggt tataagcaac
    27241 ggtgatggga tcttcaacat ccagcacagc acttacctgg tggctcacat cagcacaatc
    27301 atcaacattg atgccatctt cactatcaat atagatgcgc agtgtggatg tgcgaccgcg
    27361 aataaattcg atgcctacca gttcaaaacc cagggcctca actggcgcag taatcatctc
    27421 tgttaatttt tgctctaatg tggacaagcc cacccccaag acataaaaaa agggcctaaa
    27481 gcccagttat tctgtagtca gataacaaaa aaccccgata aatcggggct ttatataact
    27541 gaaccctata accgcaactg cggtctggag cactttccag aaggattttt tcaaatccca
    27601 ctacgaaggc cgaagtcttc acagtatatt tgaaaaagga ctctaaggga aagtggttgc
    27661 gggggccgga tttgaaccga cgaccttcgg gttatgagcc cgacgagcta ccaggctgct
    27721 ccaccccgcg cctgaaacgt ggcaaattct actcgttttg ggtaaaaaat gcaaatactg
    27781 ctgggatttg gtgtaccgag acgggacgta aaatctgcag gcattatagt gatccatgcc
    27841 acattttgtc aacgtttatt gctaatcacg tgaatgaata tccagttcac tttcatttgt
    27901 tgaatacttt tgccttcccc tgctctccct taagcgcatt attttacaaa aaacacacta
    27961 aactcttcct gtctccgata aaagatgatt aaatgaaaac tcatttattt tgcataaaaa
    28021 ttcagtgaga gcggaaatcc aggctcatca tcagttaatt aagcagggtg ttattttatg
    28081 acgacgattc tcaagcatct cccggtaggt caacgtattg gtatcgcttt ttccggcggt
    28141 ctggacacca gtgccgcact gctgtggatg cgacaaaagg gagcggttcc ttatgcatat
    28201 actgcaaacc tgggccagcc agacgaagag gattatgatg cgatccctcg tcgtgccatg
    28261 gaatacggcg cggagaacgc acgtctgatc gactgccgca aacaactggt ggccgaaggt
    28321 attgccgcta ttcagtgtgg cgcatttcat aacaccaccg gcggcctgac ctatttcaac
    28381 acgacgccgc tgggccgcgc cgtgactggt accatgctgg ttgctgcgat gaaagaagat
    28441 ggcgtgaata tctggggtga cggtagcacc tataaaggaa acgatatcga acgtttctat
    28501 cgttatggtc tgctgaccaa tgctgaactg cagatttaca aaccgtggct tgatactgac
    28561 tttattgatg aactgggcgg ccgtcatgag atgtctgaat ttatgattgc ctgcggtttc
    28621 gactacaaaa tgtctgtcga aaaagcctac tccacagact ccaacatgct tggtgcaacg
    28681 catgaagcga aggatctgga atacctcaac tccagcgtca aaatcgtcaa cccgattatg
    28741 ggcgtgaaat tctgggatga gagcgtgaag atcccggcag aagaagtcac agtacgcttt
    28801 gagcaaggtc atccggtggc gctgaacggt aaaaccttta gcgacgacgt agaaatgatg
    28861 ctggaagcta accgcatcgg cggtcgtcac ggcctgggca tgagcgacca gattgagaac
    28921 cgtatcatcg aagcgaaaag ccgtggtatt tacgaagctc cggggatggc actgctgcac
    28981 attgcgtatg aacgtctgtt gaccggtatt cacaacgaag acaccattga gcagtatcac
    29041 gcgcatggtc gtcagttggg ccgtctgctg taccaggggc gttggtttga ctcccaggcg
    29101 ctgatgctgc gtgactctct gcaacgctgg gttgccagcc agatcactgg tgaagttacc
    29161 ctggagctgc gccgtgggaa cgattattca atcctgaata ccgtctcaga gaacctgacc
    29221 tacaagccag agcgtctgac gatggaaaaa ggcgactcgg tgttctcgcc agatgatcgt
    29281 attggtcaat tgaccatgcg taatctggat atcactgata cccgagagaa actttttggt
    29341 tatgccaaaa ctggcctgct ttcctcctct gccacttcag gcctgccgca ggtggagaat
    29401 atggaaaaca aaggccagta attcgacatc aaccctgccc ttcaggggtg gggttacctt
    29461 gcttatcctc tgtaaactcc tttctgagca tcacataatc gaaaacatca tggtttgcat
    29521 ccacaacccg tgagtctcct ttatagtgtg caatcacttc ctcaagcgtt tgcggctgtt
    29581 ccggctttgt tacccccatc cacgcattaa taaggtaatt attgtaagct gtcgaaaaga
    29641 tattgttttc cgttgtgcga tccacgccat ccccaagaac ggggctatac cagataaaca
    29701 tcgggacatg atatgcctgc tggctagcct ccctgcctcc atgaaaatag acgttcttct
    29761 tcgtagggtc acgttccagg ccgtggtcgg caaaatacat gaccgaggcg cggcgatctt
    29821 ttaataattc aaaaacctga cctagcaaac tatctgtgta atgaatggag ttgtcatagc
    29881 aggcatcctg atcgtcctgc ggttgaaaca cggcgctgga ttgcggatag gcgctacaag
    29941 ccggttcatg gcttccattt aaatgaagaa caatcagttt cttttgctgc gtattttgct
    30001 gtaatgcttg cgataaatgc ggcaacaaca attcatcaaa tcctctgaca taaactgttt
    30061 ccatggcgcg catggcgata ctggtaactg ctgtaccatt ctgccgaaaa gcggattgcg
    30121 agcttagcca gaaagtctga aatcctgctt gattagccat attaataatg ttgtcggggt
    30181 aattatgaat gtcatgactc aaaacagaat cagcagttaa agaaagggga accgacagcg
    30241 cagtgtaagg tgcgccgctt attgcttgat taaacagttt gatctgtttt ctttgtgctt
    30301 caacttgcgg tgtcgtagag cgtgtatatc catacaaaga catattgtcg acacgtacag
    30361 actcccccac aatcaacacg taggtatcaa tacttgtatc cctgactgat aattgaaaat
    30421 acggcaccgt gtttgcaatt gagagtaatc tttgatgcgc tttcgctgct aaagcaaaat
    30481 agttgagatt gaaaaacggc gtataggtag caaatcacga cgctagtata tatggactga
    30541 acgctttttt attttttgca tctttataag caaattgaca agcggaaaat aaactgcccg
    30601 aaatgacaat cagcaataat attcctgtca ctttttttgt cggcaaggaa acatcatatt
    30661 ttattattac tgccaaaaaa agaagggata aaaaagcaaa agcacataga taagggatat
    30721 acatccccag cattttcaca acttcatccg gatcactctg cagcacacta atcgcaaatc
    30781 catcattgaa agttgtacca aaggtacacc atgaatagag gctaatactc atgtctgccg
    30841 cagtcagtaa cacaaatggt gcggcaacaa gcaggcgaag cggaagggat gatatacgtt
    30901 taacaaccag gatggtcagg acgaagaaca gcaatcgctg aaccatatgc gctccggaag
    30961 aggcgactaa gttcgtaata ccaaaaacaa taacacaaag atacaacaac caatgagatt
    31021 taaaatcttc tagcaaattt gttgaatact gtcatccaga aatccaagtt cacaaccaat
    31081 ataaacaatt atcaacaatg ttcaatttta tatcagacat gtttttcaac tctgatccat
    31141 atcagaccga aacactgtta gttttttctt tttaaatgat gcgaccattg cgttctcagt
    31201 aattaaataa cattatcaat gcgtctctaa acattacgca gaacgtagcc atcagtagtt
    31261 atactgacag gagcaggatg gtttatcgag aagtaaacat atccgtattc gtaccggaac
    31321 tggagttgac acaataaagt gccaattatg tcagcagtag ggaaaagttt ataactaagg
    31381 cgtaccggca ccatcgtttc aaggtaccag ctacgagtaa agcaactgga cgagatacag
    31441 atacctgaca accattcatc agaccaggac caaaacgaaa agagacgctt ttcagcgtct
    31501 cttttctgga atttggtacc gaggacggga cttgaacccg taagccctat tgggcactac
    31561 cacctcaagg tagcgtgtct accaattcca ccacctcggc acggatacta ctttttagtt
    31621 cgggatatcg ctggtcggct tagccggagc agctggctga gtttgttcgg ttttcgccgg
    31681 tgcactcaga ttttcccatt cgctacctct attggttttg ttgctattga tgttacccag
    31741 caccagactg atgatgaaga ataacgttgc cagcagcgcc gtcatgcggg tcatgaagtt
    31801 accagaacca cttgaaccaa acagcgtagc ggaagcgcct gctccgaagg aggctcccat
    31861 atcagcgcct ttaccttgct gcagcatgat cagaccaaca aggccaattg ccacaataag
    31921 gaaaactact aaaagagctt cgtacataat caacctgttc cttgcggagt tgccgcgtac
    31981 caatgcttca accaataaag cgggagtttt taatctttcc cactgaagcg ggtgtgaata
    32041 ctaaccaaaa cgaatgacct tcgcaagggc aattttatcg cattgtatca actgcggaaa
    32101 aaaacagcaa aaccgatgtg ttcgccgaga aaaaaggcga caatcgccgc ctttttagcc
    32161 agttatctaa cgctttaaac ggcttttact gcatcggcga tgcggtgtgc aaattcagtc
    32221 acctgcgctt cgtcttcgcc ttccaccatc acgcgaatta acggttcggt gccggattta
    32281 cgcagcaaca cgcgtccacg gttgcccagc gcagcttcaa cctctgcggt cacggcttta
    32341 actgactcat gctcaagcgg atcgccgcta cctgcggtgt aacgtacgtt aaccagaatc
    32401 tgcgggaaca ttttcatgcc gctgcaaagg tcgtgcaggc tcatatggtt acgtgccatc
    32461 gccgccagca cctgcaagcc agcaacgatg ccgtcaccgg tagtggtttt atccagcagg
    32521 atcacatgac cggaattctc tgcaccgata cgccagcctt tctcctgcat tttttccagt
    32581 acgtagcggt cacccacttt cgcgcgcgca aatggaattc ctaactgttt cagcgccagt
    32641 tcaagcccca tgttgctcat caatgtaccc acagcaccac cacgcagctg gccctgacga
    32701 agaccttcac gcgcgatgat atacatgatc tgatcgccat cgactttatt gccttcatgg
    32761 tcaaccataa tcacgcgatc gccgtcgccg tcgaaggcaa taccgagatc cgctttttca
    32821 gccagcacac gagcctggag cgcgcgaacg tcggtagccc ccacttcggc attgatgttt
    32881 acaccgtttg gctcacaacc gatagcgata acgttcgccc ccagttcgcg cagcacgttc
    32941 ggcgcgatgt gataagtcgc accgtttgca caatccacca caatcttcag ttcactgagg
    33001 ctaagttcgt tcgggaacgt ggctttgcaa aactcgatat agcgacccgc ggcatcaacg
    33061 atacggctgg ctttacccag ttcagccgaa tcaacgcagc tgatctcctt ttccatttcc
    33121 gcttcgatgg cctcttctac cgcatccggc agtttagtgc cgtcgataga gaagaattta
    33181 atcccattat cgtagaacgg gttatgcgat gcagatatca caattccggc ctctgcgcgg
    33241 aaggtacgcg tcagataagc cacggccggt gttggcatcg ggccagtgaa gagtgcggaa
    33301 aggcccgctg ccgccagacc cgcttccagt gctgactcca gcatatagcc agaaatacgc
    33361 gtgtctttac caataataat cttacgggag ccgtggcgcg ccagcacttt acccgcggcc
    33421 caacccagct taagcacaaa atcaggtgtg atcggcgcat cccctacacg accacgaatc
    33481 ccatcggtac cgaaatattt acgattactc atagcgtttg ttttcctttg cagacagagt
    33541 ggcttccacc acccgcatcg cttctacggt ttctttgacg tcatgaacac gaatgatgtg
    33601 cgcgccttgc attgcggcaa tgaccgcaca ggccagacta ccgctcaggc gctcggacgg
    33661 ccccacgttc agcagctgcc caatcatcga ttttcgtgac atacccacca acagcggcag
    33721 gttgaaatgg tgaaattcag ccaggcgcgc cagtaatgaa tagttatggg agagattttt
    33781 accgaaaccg aatccggggt cgagcaacaa tttctctttt gcgatacccg cctgctcgca
    33841 acgtgctatt tgctcaataa agtagcgatt cacttctgca aagacatcgt catacttcgg
    33901 agcttcctgc atggtttttg gatttccctg catatgcatc agacaaaccg gtaaaccggt
    33961 ttctgcagcc gcctccagag cgccaggttc ggaaagggag cggatatcat taataatgtg
    34021 agcgccaact ttcgctgact cacggatgac ttctggtttg gatgtatcga ccgagatcca
    34081 gacttcgaag cgttgagcaa ttgcctcaac cacaggaata acacgttgca actcttcttc
    34141 aacgctaact tccgccgccc ctgggcgcgt ggactcgcca ccaacgtcaa tgatcgtcgc
    34201 gccagcattg atcatcagat tcgcatgttt caccgcatct atcagcgagt tatgcgtgcc
    34261 accatccgaa aaggaatcag gcgtgacgtt gaggatcccc attacgtgag gatggctaag
    34321 gtccagtgaa gtaccctggg caaagagttt cattatgtta tccctggtat gaattgataa
    34381 gaaaaaaccc cggagcacgc cccggggttt tcggtacaaa tacagtcatc tgatgcggga
    34441 acttacttgt cgcctaactg ctctgacatg gtgttacccg ggttcggcgt acgcggttca
    34501 tcaaccggac gaggagcctt tggactacca ttgtcgccag aattgttaga agcgcctggt
    34561 tcttcccagc ccgctggcgg acgtacatcg cgacgtgcca tcaggtcatc aatctgcggt
    34621 gcgtcgatag tctcatattt catgagagca tctttcatcg catgcagaat atccatattg
    34681 tcggtcagaa gctgacgcgc acgattatag ttacgctcaa tcagtgcttt cacttcctgg
    34741 tcgatgatac gtgcagtttc atcggacata tgtttcgctt tcgctacgct acggccgagg
    34801 aatacttcac cttcttcttc cgcgtacagc agtggaccca atttctcaga gaagccccac
    34861 tgagtcacca tgttacgtgc caggttggtc gcaactttaa tatcgttgga cgcaccggta
    34921 gaaacatgtt ccggcccgta gatgatctct tctgccagac gaccaccgta cagcgtagaa
    34981 atctggcttt ccagtttctg acggctggcg ctgattgcgt cgccctcagg caagaagaaa
    35041 gtcacaccca gcgcacgacc gcgtgggata atcgtcactt tatgcaccgg atcgtgttcc
    35101 ggcaccaggc gaccgataat cgcatgaccc gcttcgtggt aagccgttga ttctttctgc
    35161 gcttccgtca tcaccatgga gcgacgttcc gcacccatca tgattttgtc tttcgctttc
    35221 tcgaactcaa ccatcgacac aacgcgtttg ttgccacgag cagcgaacag tgccgcttcg
    35281 ttcaccaagt tcgccaggtc agcaccggag aaaccaggag taccacgagc aatgattgcc
    35341 gcgtcgatat cgggtgccaa tggtacgcga cgcatgtgaa ctttcaggat ctgctcacga
    35401 ccgcgaacat ctggcaagcc gaccacaacc tggcggtcga aacggccagg acgcagcagg
    35461 gccgggtcga gaacgtccgg acggttagtc gcggcgataa cgatgatacc ttcgttacct
    35521 tcgaagccat ccatctcaac cagcatctgg ttcagagtct gttcacgttc atcgtgacca
    35581 ccgcccagac cagcgccacg ctggcggcct acggcgtcga tttcatcgat aaagatgatg
    35641 cacggtgccg ctttcttcgc ctgttcgaac atgtcacgaa cacgggatgc acccacaccg
    35701 acgaacattt ctacgaagtc agaaccggag atagtaaaga acggaacttt cgcttcgcct
    35761 gcaatcgctt tcgccagcaa cgttttaccg gtacccggag gaccgaccat caggacgcct
    35821 ttcgggatct taccgccgag tttctggaag cggctcggct cgcgcagata ctctaccagt
    35881 tcagcaactt cttcttttgc ttcgtcgcag cccgcaacgt cagcaaaggt cgttttgatc
    35941 tgatcttccg tcagcatgcg cgctttgctc ttaccaaacg acatggcacc tttgccaccg
    36001 ccgccctgca tttgacgcat gaagaagatc cagacaccaa tcagcaacag catcgggaac
    36061 caggagatga agatagaagc cagcaggctt ggttcttcag gcggttcacc gacaaccttg
    36121 acgttcttgg tcaacaggtt atccagtaat ttcggatcct gaaccggaat gtaagtggta
    36181 taacggttac tatctttctt ggtaacgttg atttcacgtc cgttgatacg cgcttcacga
    36241 acctggtcgt tattgacctc ttgtaggaag gtagagtaat ccaccttacg gccattagac
    36301 tcgctgggcc caaagctctg gaatactgac atcagcacaa cggcaatgac cagccagagt
    36361 attaggtttt tcgccatgtc actcaaggga ttaacctctt attacaactg tgttaaaaac
    36421 agcgtcagga tactctatat ccagcatctt tcaaactttc gtctgaaatc tcccggttag
    36481 ggtttacgcc cggtcgctac aatatacact tctcgcgaac gtgcacgaga agagtccggc
    36541 ttacgaactt tgaccttcgt aaacagggag cgaatttccc ttagatactc atcgaaacct
    36601 tcgccctgga acaccttcac tacaaaactg ccacctggcg ctaatacatc acgacacatt
    36661 tctagcgcca gttccaccag atacatggct cgggggatat ccaccgccgg tgttccgctc
    36721 atgtttggtg ccatatcgga catgacaacc tggactttgc tgtcgccaac gcgctccagc
    36781 agtgctttca tcaccagttc atcacgaaaa tcgccctgaa gaaagtccac accaacgata
    36841 ggatccatag gtaaaagatc gcaagcgatg atgcggcctt tgccgccaat ttgggtgacc
    36901 acatattgtg accaaccacc cggagcagca ccaaggtcga caaccgtcat tcccggttta
    36961 aagagtttgt cactttgctg tatttcatca agtttaaacc aggcacggga acgtaatccc
    37021 tttttctgtg cctgttgaac atatttatcg ctaaagtgtt cctgaagcca gcggctggag
    37081 ctggcagaac gcttcttacc tgtcatttaa ctttcccatg gggataactc atcgtaacca
    37141 attgcgtaaa tttttacgcg cctatttggt gatatatggg agatggcggt agaatgaccc
    37201 gttttcaatc ccaacgtaag caaaaaatac gatgaatctg agtactaaac aaaaacagca
    37261 cctgaaaggt ctggcacatc cgctcaagcc agttgttctg cttggcagta atggtttgac
    37321 cgaaggggtg ctggccgaga ttgaacaagc gttagagcac catgaactca tcaaggtgaa
    37381 aatcgccacc gaagatcgcg aaacaaaaac cttgatcgtg gaagctatcg tgcgcgaaac
    37441 cggcgcctgt aatgtacagg tcatcggtaa aacgctggtg ctttatcgcc caactaaaga
    37501 acgtaaaatc tcgctgccac gctaagatta tcctaaagtt acacacattt gctgtgtaaa
    37561 acgaggggtt ttccgcaggc aggagagcaa aatgccacgc tctgttcgtt gataaaaggc
    37621 cgcagagcgg cctttttcct ttctttacaa tacatcaaca tcttgagtat tgggtaattc
    37681 ttacaggtat tccaccttaa ttacttcaaa ttctacttcg ccgcccggcg ttttgatgac
    37741 cacaacatca tcttcttctt tgccgatcag gccacgagca ataggcgagt ttacagaaat
    37801 caggttttgt ttaaagtcag cttcgtcatc gccaacgatg cgataagtct gttcttcgtc
    37861 agaatccaga ttcagcaccg ttacggtagc accaaaaata acgcgcccat tgttgggcat
    37921 tttggtgaca tcaatcacct gcgcgtttga cagcttggct tcgatgtctt tgatacggcc
    37981 ttcgcagaag ccctgctgtt cacgagctgc gtggtattcg gcgttttctt tcaggtcgcc
    38041 atgctcacgc gcttccgcga tagcagcaat gatttcagga cggcgcacag atttcagaaa
    38101 atccagctct tcgcgtaatt tttcagcgcc gcgtaaggtc atcggaatag cttgcatttg
    38161 ttatacctct tgaatattcc tgatggggca agtcttcacc caccccggct gttcggcctg
    38221 cccggcataa ctcctcaccg ggaccagaag caaaaaaata ccgacccggg tacaagtccc
    38281 aggtcagcta caattcacat tttgatggtc attttaccct gaagttcccg aagggtcatc
    38341 gtttacttta tagggcgttg cgccgtagta tgacggctcg attccaggtt gttagcgcga
    38401 gattatgcga ttttccagat ttatcatcgg attgaccagc tgtatagcgt tcagtgttca
    38461 ggccgcgaat gttgatgagt acgttactca actccccgca ggtgccaacc ttgccctgat
    38521 ggtgcaaaaa gtcggtgcgt cggcccccgc tattgattac cacagtcagc agatggcgct
    38581 gcccgccagt acccagaaag tgattactgc gctggcggcg ttgattcaac tcggccccga
    38641 ttttcgtttc accacgacgc ttgaaaccaa aggcaatgtg gaaaacggcg tacttaaggg
    38701 tgacttagtg gcgcgatttg gtgccgatcc gacgttaaaa cgtcaggata ttcgcaatat
    38761 ggtcgcgact ttgaaaaaat ctggcgtcaa ccaaatcgat ggcaatgtgt tgatagatac
    38821 ctccattttc gccagccacg ataaagcccc cggctggcca tggaatgaca tgacacaatg
    38881 ctttagcgct ccgcctgctg ccgccatagt tgaccgcaac tgtttctccg tctcgctcta
    38941 cagtgcccca aagcctggtg atatggcttt tatacgcgtg gcatcttatt accccgttac
    39001 gatgttcagc caggtacgca ccctcccccg tggttctgcc gaagcgcaat actgcgaact
    39061 ggatgtggtg ccaagcgacc tgaaccgctt tacgctgacg ggatgcctgc cacaacgttc
    39121 tgagccgctc ccgttggctt ttgccgtgca ggatggagcc agctatgccg gtgcaattct
    39181 gaaagatgag ttaaaacagg cgggtatcac ctggagcgga acactgctgc gccagactca
    39241 ggttaacgaa cctggaacgg tagttgccag taaacagtcg gccccgctgc acgatctgct
    39301 taagattatg ctgaaaaagt cggacaacat gatcgccgat acggttttcc gcatgatcgg
    39361 ccatgcgcgc ttcaatgtgc ctggaacatg gcgggctggg tcggacgccg tgcgtcagat
    39421 cctgcgccag caagccggtg tcgatattgg taacaccatt attgccgatg gttcagggct
    39481 ttcgcggcat aacctgattg cccccgccac catgatgcag gtgctgcaat acattgccca
    39541 acacgacaat gaacttaact ttatctccat gctgccgctt gcgggctatg acggctcttt
    39601 gcagtaccgt gcaggtctgc atcaggcggg cgtggatggc aaagtctcag cgaaaaccgg
    39661 ttcgttgcag ggggtatata acctggcggg attcattacc acagcgagcg ggcaacgaat
    39721 ggcatttgta caatatcttt ctggctatgc agtaaaaccc gcggatcagc gtaatcgccg
    39781 tattccgtta gtgcgttttg aaagccgttt gtacaaagat atttatcaga acaattagtc
    39841 aaaaagaaac cccggcgcat ggccggggtt tcagattatt gacaaagtgc gctttgttta
    39901 tgccggatgc ggcgtgaacg ccttatccgg cctacaaaat cgtgcaaatt caatatattg
    39961 caatcatctt gtaggcctga taagtgtagc gcatcaggct tatttggcat tggtcattag
    40021 tgattaacgc ttgtaaatga actcaacgcc ttcttcgtcg tcttcgtccc agtcgtcatc
    40081 ccagtcttca tcatcctctt cagcaatctc ttcaagctgc tgacggtgat aatcatccca
    40141 catgaattcg actttctctg gctgtttcgc ttcttcagcc tgcacgaccg ggttttcaat
    40201 gataaaggtc atcacatccc agcagagatc tttcacgccc agtccgctcg ccgcagagat
    40261 cagataatat ttatcttccc agcccagcgc ttcagcgatc gctttcgctt tctcttcggc
    40321 ttctgcctta tccagcagat cgatcttgtt gaagactaac caacgcggtt tcgccgccag
    40381 atcctggctg tatttttcca gctcgctgat aataatacgc gcgttttcaa ccggatcggt
    40441 gccgtcaatc ggatcgatat cgatgaggtg caacaggacg cggcaacgtt ccaggtgctt
    40501 caggaagcga atgcccagac ctgcgccttc cgcagcgcct tcaatcagtc ctggaatatc
    40561 ggcaacaacg aagctctttt cgttgtccat tcgtaccaca cccagacttg gcaccagagt
    40621 ggtaaacgga taatccgcca ctttcggttt agccgccgat accgcacgaa taaaggtcga
    40681 tttacccgcg tttggcatcc ccaacatacc gacgtcagcc agcagcatca gctccagcag
    40741 cagctcacgc ttatcgcccg gcgtaccgtt agttttctgc cgtggtgtac ggttaactga
    40801 cgatttgaaa cgggtattgc ccagaccgtg ccagccgccc ttagcaacca gcagacgctg
    40861 accgtgttta gtcatatcac ccatggtttc accagtaccc tggtcgatta cacgcgtacc
    40921 taccggcact ttaatcgtca cgtctttacc gcgcttaccg gtacaatcgc ggcttgcgcc
    40981 gttctgaccg cgctctgcac ggaaagattt ttcaaaacga taatcgataa gcgtgttcag
    41041 gttctcgtcg gcttccatcc atacgtcacc accatcaccg ccgtcgccgc catccgggcc
    41101 gcctttcgga atatactttt cgcggcggaa gctcacgcaa ccattaccgc catcacctgc
    41161 aacgaccaga atcgatgctt catcaacaaa cttcatttta ttctccgtaa atcattcgcc
    41221 tgcgcggggt tgcgatacca ccgttgtatg cttacgtaat ccgccccaaa tacgatgacc
    41281 aatggcggaa tacatcgcgc ccgcaaccac gacaaacgca ccgagataac ctaaaaggtt
    41341 taacatcggt ctggcgaaga aatcgggcca ggccagtgat aaaagatctg aaaaaaacag
    41401 cgtaaacagt ggggtgagcg tgatgatcgc gctcacctgc gctgcctgcc agcgagccat
    41461 cgcttccgcc aaggcgccat atcctaccaa ggtattcagt ccgcaaaaaa ttaaacatgc
    41521 gagctgccag tggctaagct gcgctatcac tccaggcttt gccagaggga agagcgcgat
    41581 tgtacataaa gtgtacagta aaaacaggat ctgcggtgag gccagccgac gcaataaaac
    41641 cttttgcgcc acgccataac tcacccaaac cgtcgccgca ccgaccccaa agataactcc
    41701 ccaggtgtaa tcggtgagct ttgtaaatat ctcgaccaga ctggtgttaa aaaacatcac
    41761 cagaccgctc aggagcatca atgcccctac aacctgagtg ctgcgcattt tctctttcag
    41821 gataaatacg ctggcaacca tcatgccaac tggcgagagt tgcccaatca cctgcgaagc
    41881 ggtcggactc aggtattgca aggatgagct gaacaggatg aagttcccaa acagcccggc
    41941 ggtcgccact gccaacaaaa tcaaccagcg tggcttacga aacacgcgta atggcggcaa
    42001 cctcttcttc accgcaagaa tggcaccaag gccaatactc gccatcaaga aacggtaaaa
    42061 cacgattgtc ggaggttcca tcacctccag cacctgcttc attgcgattg gcaacgcccc
    42121 ccagcaaatt gctgtggtaa gcgccaaaag aatgccaatg cctgcctgct gcttcatacc
    42181 gtattccctg caaagcgcat ttgcggatta acgtcgcacc atgcgcattt ttaccggtta
    42241 tcgaatgtaa aaagccccgc aacgtgttgc ggggctttca tccgttaccg ggacgcgaaa
    42301 aacttattca gcttcgatgc tgataaattt acggtttttc gggcctttaa cttcgaattt
    42361 cactttaccg tctgctttag caaacagagt gtggtcacga ccgcaaccta cgttagcgcc
    42421 agcgtggaat ttggtaccac gttgacgaac gatgatgcta cccgccagaa cggattcgcc
    42481 accgaaacgc ttaacgccca agcgtttagc ttctgaatcg cgaccgttac gtgtggagcc
    42541 gccagccttt ttatgtgcca tttgaaatct ctcctcaggt cttaggcgct gatgccagta
    42601 attttcacat cagtgaacca ctgacgatgg ccctgctgct tacgatagtg tttacgacga
    42661 cgaaacttaa cgattttaac tttctcgcca cgaccgtgag caacaacttc agctttgatt
    42721 acgccgccat caacgaaagg aacgccgatt ttgacttctt caccgtttgc gatcatcagc
    42781 acttcagcga actcaacagt ttcgccagtt gcgatgtcca gcttttccag gcgaacggtc
    42841 tgaccttcgc ttactcggtg ttgtttacca ccactttgga aaaccgcgta catataaaac
    42901 tccgcttccg cgcacacctt ttcaatgatt cagagtgcgc tataaatatt cacaataggg
    42961 cgcgaatatt acgcaaaacg cacgcctttg acaagtgcta cagtcaatac acgaagaaaa
    43021 aaacacaact tgtacggtaa cgtttatctg tgccattttt tcagtacaat taccctatat
    43081 tcctaaccat aaaccctaag ttgcctttgt tcacagtaag gtaatcgggg cgaaaagccc
    43141 ggcttttgcg atgaatttag aaaaaatcaa tgagttaacc gcgcaagata tggcgggtgt
    43201 taatgcggca atccttgagc agcttaattc cgacgtccaa ctgatcaatc agttaggcta
    43261 ttacatcgtc agcggcggcg gtaaacgtat tcgtccgatg attgctgtac tggctgcacg
    43321 agctgttggc tatgagggaa atgcgcatgt caccattgct gccctgatcg agtttatcca
    43381 cacggcgact ctgctacacg acgacgttgt ggatgaatca gatatgcgca ggggtaaagc
    43441 taccgccaac gccgcatttg gcaatgccgc cagcgtgctg gtaggcgatt ttatttatac
    43501 ccgcgcgttc cagatgatga ccagcctcgg ttcactcaaa gtgctggaag tcatgtcaga
    43561 agctgtaaac gtcatcgcag aaggtgaagt tctgcaactg atgaacgtta acgatccgga
    43621 catcactgaa gaaaactaca tgcgcgttat ctatagcaaa accgcgcgtc tgtttgaggc
    43681 tgccgcgcag tgttccggga ttctggctgg ctgtacgccg gaggaggaga aaggcctgca
    43741 ggattatggg cgctatctcg gcactgcttt ccagttgatc gacgatttac tcgattacaa
    43801 tgccgatggc gaacagttag gtaaaaacgt cggcgacgat ctgaacgaag gtaaaccgac
    43861 gctgccgctg ctgcatgcga tgcatcatgg cacaccagaa caggcacaga tgatccgtac
    43921 cgccatcgaa caaggtaacg gtcgccatct tctggaaccg gttctggaag caatgaacgc
    43981 ttgtggatct cttgaatgga cgcgtcagcg cgccgaggaa gaagcagaca aagccatcgc
    44041 agcgttacag gtgctcccgg acaccccttg gcgagaagca ctcatcggcc tcgcgcacat
    44101 cgctgttcaa cgcgatcgtt aatcccctcc cttcatcccg cgcagtgcgc gggatgattc
    44161 cagtaaattc cataaaaaca ctaattcagc gctataacac ctgaaaaaag ggcgatgaat
    44221 ctttatatat tctgaatatt cacactcttt acaggaactt tttagcgcaa tagaccatca
    44281 ggagtatagt gatgcccgac agaagaagtg ttctgaatga aagcgactaa cttaaggagt
    44341 gaggaaagat ggaaagtaat ttcattgact ggcatcccgc tgacatcatt gcaggtttgc
    44401 gcaagaaagg aacgtcaatg gcggcggaat ctcgcagaaa tggtttgagt tcctcaacgc
    44461 tggcaaacgc attatcgcgc ccatggccga aaggagagat gattattgcg aaagccctgg
    44521 gaactgaccc ctgggttatc tggccatcac gctaccatga tccgcagacc catgagttta
    44581 tcgacagaac gcagttgatg cgtagctaca ctaaaccgaa aaaatgagtg gtctggcggt
    44641 agccccgcga acggggctgc cagctctcag acgattactc gcctttcaca cgctcaatat
    44701 ttgcacctaa agcgcgcagt ttgtcttcaa tgcgttcgta gccacgatcg atgtgataaa
    44761 tacgatcaac caccgtcgtc ccttccgcaa tacagccagc cagcaccagg cttgctgatg
    44821 cacgcagatc ggttgccata acctgtgcac cagaaagttt ttcaacacca tgacaaataa
    44881 cggtattgct ttcgatttcg gcgtgcgcgc ccatacggct cagctctggc acatgcataa
    44941 agcggttttc aaagaccgtt tcggtgatga acccggtccc ttcagccacc aggttcaaca
    45001 gcgtgaactg ggcctgcata tcggtcggga atgccggatg cggcgcggta cgtacgttaa
    45061 cagccttcgg acgtttgcca tgcatatcca ggctaatcca gtcttcgccg acttcgatgt
    45121 ccgctccagc atcacgcagt ttcgccagca cggcgtccag agtatctggc tgcgcgttac
    45181 ggcagataat tttgccacga gagatcgccg ccgccaccag gaaagtaccg gtttcgatac
    45241 gatccggcag cacgcgatag acaccgccgc ctaaacgttc cacaccttcg atgacgatac
    45301 gatcggtgcc ctgaccgcta attttcgcac ccagcgtaat caggaagttc gcggtatcga
    45361 cgatttccgg ttcacgcgct gcgttttcaa taatcgtggt gccttccgcc agggttgcag
    45421 cacacatgat ggtcaccgtt gcgccaacgc tgactttatc catcacgata tgtgcgcctt
    45481 tcagacgacc atcgacggaa gctttaacgt aaccttcttc cagtttgatg gtcgcgccta
    45541 attgttcgag gccagaaatg tgtagatcaa ccggacgcgc accgatcgta caaccgccag
    45601 gtagtgaaac ttgcccctga ccaaagcgcg ctaccagcgg ccccagcgcc cagatagaag
    45661 cacgcatggt tttaaccaga tcgtaaggtg cgcagaatac attaacgtcg cgggcatcaa
    45721 tatgcacaga accattacgt tctactttcg cacccagctg gcttagcagt ttcattgatg
    45781 tatcgacgtc tttcagtttc gggacgttct ggatctctac cggttcttcc gccagtagtg
    45841 cggcaaaaag aataggcaga gcagcatttt tagcgccgga aattgtgact tcgccctgga
    45901 gcttcgttgg cccctgaaca cgaaatttat ccatttagtt tgttctcagt taacaattca
    45961 tatccgctac cggcgaatcg cccatagctc aaaagccgtt cagtttgcga tcgcgcgccc
    46021 actccgcagg ggtatacgct ttgatcgaca cagcatgaat gcggttatcc gcaatatatt
    46081 ccatcagcgg gccatagacc gtctgctgtt ttttaacccg actcataccg tcaaacaact
    46141 cacccacggc aataacctga aagtggctgc catcgccgga aacgtggact tcctggaggg
    46201 agagagcgtt catcaacacg ctctgaattt cattattttc catgggatct tcaatcatca
    46261 gttaataaac cagcgaaaca tcttagagca aagttgcgct ggcataaata agcaaaaagc
    46321 ctcgctgata aatcagacaa ggctcgactt gcaggcaggt ttgccggaca ggcggttaac
    46381 gccatatccg gcctgaaaaa atttaacgag gcagaacatc agcaggcaaa ttatacaatt
    46441 tcgcgagggt atacactttg tcgtttaccc cctgaagcgt cacgttgttg ccctgctttt
    46501 tcgccagatc gataagatgg agcagcagtg ccagtccccc cgtatccacg cgggacacac
    46561 ggctaagatc gatgcaggta atccccttca ccgcctcctc acgcatttcc caaagcggta
    46621 gcagaacgtc ctgatccagc tctccggata acgccagcgt gtcacccgtc tgcatccagc
    46681 tcagtgactc gctcattatt ttttctcttc cagagtgatt ttctgttgag aaatcgattt
    46741 cagttgcgca gtcaggccgt cgataccttt ggtacgcagc agcgttcccc actcgttttg
    46801 tttggtggtg atcatactga cgccttcagc aatcatgtcg taagcttgcc agtttcccgt
    46861 ctgggagttt ttacgccact ggaagtccag acgcaccggc ggacggccat tcgggtcaat
    46921 aatggtaacg cgaataggca caatggtttt atcgcccagc ggctgttctg gcgcaatctg
    46981 ataggtttga ccgtgataca tcgccagcgc ctgaccgtaa gcctgcttca ggtactcacg
    47041 gaaagcggca aagtaggctt cacgttgagc aggggtcgca ctcttgtaat actggcccag
    47101 caccagcgca ccggcgtatt tcacctgtac gtatggcagc agttcctgat caacaatggt
    47161 acgcagataa tccgggttgg cccgaatttg cggttgctca ttcttcaggc gatcgaacgt
    47221 tttctgcgcc gcctcgtcca tcagcttata aggattggtc tggtctgccg cggttgccgc
    47281 actcagaggt gcaatcacca gcaaagcgac catcattaaa cgtttaaaca tgcgtcggtt
    47341 ctcctgaaat tatttcgttg tacccacagg ttcagtggtt tcattattac ctggcgcagc
    47401 agctggcgca tcgccactat tcttattgtc atcgccttta ctaccgtaaa ggaactgacc
    47461 aatgagatct tccagcacca tcgcagactt ggtgtcctga attgtatcgc catccttcag
    47521 gatagcagtc cccagttccg ggtcttcaaa accgacgttt aatgccagat attgttctcc
    47581 cagcaggccg gaagtacgaa tgctcagcga actggtatca ggaatgtggt tataacgttg
    47641 ttcaatttcc agcgttacgc gcggcagata ggttttcggg tccagcgtaa tatccgccac
    47701 ccggcccaca acaacaccac caatgctgac cggagagcgc gctttcaggc cgccaatgtt
    47761 atcgaacgtc gcataaagcg tgtaggtcga ttcagtacgt atggacgtca cgttcgccgc
    47821 cttcaggcaa acaaacagcg ccgccagcag tgctgctaat aaaaagatac ccacccaaat
    47881 ttcatttttt ttcgtttgca tgaactcaat tcccaaacat caatgcggtc agcacaaaat
    47941 ccagccccag aacagccaga gacgagtgga caacggtgcg agtggttgcc cggctaatcc
    48001 cggcagacgt cgggatggcg tcgtagccgt taaacaacga aatccacgtc accgtgatgg
    48061 cgaacaccac gctcttaatc agacagttga ccagatccat acgccagtcg acggcatttt
    48121 gcattgccga ccagaagaac ccgctatcaa tgcctttcca actgacgccg accagcgatc
    48181 cgccccagat ccccacggcg acgaagataa ccgtcaacag tggtaatgaa ataaccccag
    48241 cccagaaacg gggagaaata acccgacgca gcggatccac cgccatcatc tccatactgg
    48301 agagttgctc tgtagcgcgc atcaggccga tttctgcggt taacgccgaa ccagcacgcc
    48361 cggcaaacaa cagcgcggca accaccggcc ccagttcacg cagtagcgat aacgccacca
    48421 gcatacccag actggtttcc gcgctataag tggtcagaac cagataacct tgcagcccca
    48481 acaccattcc gatgaacacg ccagaaacta caataatcag catcgacagg acgccgacat
    48541 tatagagctg gcgcaccagc agcggcgcat gtttgcgaaa ttccggtttg ccgaccagcg
    48601 cattgaataa cattaacccg gcccgcccga acgttctcag ggttttaatc cctttatgtc
    48661 cgagcgacgc cagcgcattt aacagcatga gtggcttaac tccctggtaa aagatcagcg
    48721 tgataatcgc cggcaggata gcggaacgga acaggcccgt cagctatccc gtccagaaac
    48781 tgacgtacgc gcggatcagg attcgtctgc aacgcctggg cactgccatg agcgacaatt
    48841 tttttgtccg ccaggatcca ggcgtgatcc gcaatactta acacttccgg cacatcgtga
    48901 gaaaccacca cacaagtcac gcccagcgcg ctgttcagct cagaaatcag cttcaccagt
    48961 acgcccatgg tgatgggatc ttgcccaaca aaaggttcat caaacatgat gagatccggc
    49021 tccagcgcaa tcgcacgcgc cagcgctgca cgccgcgcca tcccaccgga aagttcagaa
    49081 ggcattagtt tagccgctcc acgcagcccc acggcctcca gcttcatcat caccgtacta
    49141 tgcaacaatg gcgcgggaag ttgggtatgt tcgcgcagtg gataggcgac gttgtcaaat
    49201 acgttcatat cagtgaacaa cgccccggac tgaaataaca tgctcatccg tttacgcact
    49261 gtatacaggc gcgaacgaga catcgccgga atattctcac catcgaaaag gatctcacca
    49321 tgatctggtg cgatttgccc gccaatcaga cggagtagcg tcgttttacc gatgcccgat
    49381 ggccccatga tcgccgtgat cttccctcgc ggcacggtca gggaaatatt atcgaagatg
    49441 cagcgattgc cacgcgtaaa actgacatcg cgcatatcga ctaaattcgc cacagactgc
    49501 tccataattc acccttcgtc ttgcgttgat tttctaagca tggcgctcaa tttaaccttg
    49561 aacccaacat atttacagaa tattacccgc cgtggttagc gaaagctggc atttgtttta
    49621 ctttttagcc gcataaagtc aaaattaaga atccgttacg gctttctgaa aatcttcagc
    49681 ggaccggcga gtatacctga agaaaggacg ttagatgctt ttagctacgg cactgttaat
    49741 tgttggttta cttttggtcg tttacagtgc cgaccgcctg gtttttgccg cgtctattct
    49801 ttgccgaacc tttggcatcc cgccgctgat catcggcatg acggtggtca gtattggtac
    49861 atcgttacca gaaatcatcg tctcgcttgc cgcgtctctg cacgaacaac gcgatttagc
    49921 cgttggtaca gccctcggct caaacattat caatatattg ctgatcctcg gtctggctgc
    49981 gctggttcgt ccttttaccg tccattccga tgttctgcgc cgtgaattac ccttaatgtt
    50041 gttggtcagc gtggtggccg gttccgtact ctatgacgga caacttagtc gaagcgatgg
    50101 tatctttctc ctctttctgg ctgtgctatg gctgctgttc attgttaaac ttgcacgtca
    50161 ggggactgac agcctgacca gagagcagct tgcagagctg ccgcgtgacg gcggattgcc
    50221 cgtcgcgttt ttatggctcg gcattgcgct tatcatcatg ccagtggcca cgcggatggt
    50281 ggttgataac gccacggtgc tggcgaatta ctttgccatc agcgagttga cgatggctct
    50341 gacggcaatt gctatcggaa ccagtctgcc ggaactggca accgcaatag cgggggttcg
    50401 caaaggtgaa aacgacattg ctgtcggaaa tatcattggc gcaaacattt ttaatattgt
    50461 catcgtgttg ggtttacccg cgctgataac gccaggagag attgatccac tggcgtacag
    50521 tcgtgactac agcgtgatgt tgctggtgag cattattttt gcgttgctgt gctggcggcg
    50581 ctccccgcaa ccgggccgtg gtgtaggggt attattaact ggcggattta tcgtatggct
    50641 ggcgatgttg tactggttat cgccaatact cgttgaataa ctggaaacgc attatgtcgc
    50701 acgtagagtt acaaccgggt tttgactttc agcaagcagg taaagaagtc ctggcgattg
    50761 aacgtgaatg cctggcggag cttgatcaat acatcaatca gaatttcacg cttgcctgtg
    50821 aaaagatgtt ctggtgtaaa gggaaagttg tcgtcatggg gatggggaaa tcggggcaca
    50881 tcgggcgcaa aatggccgca acgtttgcca gcaccggtac accttcattt ttcgtccatc
    50941 ctagtgaagc cgcgcatggt gatttaggca tggtcacccc acaggatgtg gtgattgcta
    51001 tctctaactc aggtgaatcc agcgaaatca cggccttaat tccagtgctt aagcgtcttc
    51061 acgtaccgtt aatctgcatc accggtcgcc cggagagcag catggcgcgc gccgcagatg
    51121 tgcatctgtg tgttaaagta gcgaaagaag cctgtccgtt agggctggca ccgaccagca
    51181 gcaccaccgc cacgctggtt atgggcgatg ccctcgctgt cgcgctgtta aaagcacgcg
    51241 gctttactgc tgaagatttt gcgctctcac acccaggcgg cgcactgggt cgtaaacttc
    51301 tgctgcgcgt aaacgatatt atgcatacgg gcgatgagat cccgcatgtt aagaaaacgg
    51361 ccagtctgcg tgacgcattg ctggaagtta cccgcaaaaa tcttggtatg actgtcattt
    51421 gcgatgacaa tatgatgatt gaaggcatct ttaccgacgg tgatttacgc cgtgtcttcg
    51481 atatgggcgt ggatgttcgt cagttaagta ttgccgatgt gatgacgccg gggggaatac
    51541 gtgtgcgccc tggcattctg gccgttgagg cactgaactt aatgcagtcc cgccatatca
    51601 cctccgtgat ggttgccgat ggcgaccatt tactcggtgt gttacatatg catgatttac
    51661 tgcgtgcagg cgtagtgtaa agattcaagg ataaacaaca atgagcaaag caggtgcgtc
    51721 gcttgcgacc tgttacggcc ctgtcagcgc cgacgttatg gcaaaagcag agaacattcg
    51781 tctgctgatc ctcgacgttg atggcgtact gtcagatggc ctgatttata tgggcaataa
    51841 cggcgaagag ctgaaagcgt tcaatgttcg tgacggttat ggcattcgtt gtgcgctcac
    51901 ctctgatatt gaagtcgcta tcattaccgg gcgaaaggct aaactggtag aagatcgttg
    51961 tgccacattg gggatcactc acttgtatca ggggcagtca aacaaactga tcgcctttag
    52021 cgatttgctg gaaaaattgg cgattgcccc ggaaaatgtg gcttatgttg gcgatgatct
    52081 catcgactgg ccggtaatgg aaaaagtggg tttaagcgtc gccgtggccg atgcgcatcc
    52141 actgttgatc ccgcgcgccg attacgtgac gcgcattgct ggcggtcgtg gcgcagtgcg
    52201 cgaagtttgc gacttattac tcctggcgca gggcaaactg gatgaagcca aagggcaatc
    52261 gatatgagta aagccagacg ttgggttatc attgtgctat cactggcggt tctggtgatg
    52321 atcggcatta atatggccga aaaagacgat accgcccagg tggtcgtcaa caacaatgat
    52381 cccacctata aaagcgagca tacggacacg ctcgtctata acccagaagg ggcactaagc
    52441 tatcgattga ttgctcaaca cgttgaatat tattccgatc aggccgtttc gtggtttacg
    52501 cagccggtac ttaccacgtt tgataaggat aaaatcccga catggtccgt aaaagcagat
    52561 aaagccaagc tgaccaatga ccggatgctc tatttatatg gacacgttga agtcaacgca
    52621 cttgtgccag actctcaact tcgcagaatc acgacggata acgcgcagat caatctggtg
    52681 acgcaggatg ttacctctga agacctcgtc acgttatacg gaacaacatt taactccagc
    52741 ggtctgaaaa tgcgcggcaa cttacgcagc aagaacgccg agctgattga aaaggttaga
    52801 acatcctatg aaattcaaaa caaacaaact cagccttaat cttgtgcttg ccagctcact
    52861 tctggccgcc agcattccgg catttgccgt aaccggagac actgatcagc cgatccacat
    52921 tgaatcggac cagcaatctc ttgatatgca aggcaacgtg gttaccttta ccggtaatgt
    52981 catcgtcacc cagggcacca tcaaaattaa tgccgacaaa gtggtcgtta cccgtccggg
    53041 cggcgaacaa ggtaaagaag tgattgacgg atacggtaaa ccggcaacgt tctaccagat
    53101 gcaggacaac ggtaaacccg ttgaaggtca cgcttcccag atgcactacg aactggcaaa
    53161 agattttgtc gttctgacgg gtaatgctta tctgcagcag gtcgatagca acattaaggg
    53221 cgataagatc acttacctgg tgaaagagca gaaaatgcag gctttcagcg acaaaggcaa
    53281 gcgcgtaacc accgttctgg tgccgtcgca gctgcaggac aaaaacaaca aaggccagac
    53341 cccggcacag aagaagggta attaattcgt tatggcaaca ttaactgcaa agaaccttgc
    53401 aaaagcctat aaaggccgtc gcgtggtaga agacgtcagc ctgaccgtca actccgggga
    53461 aattgtcggt ctgctggggc caaacggtgc cggtaagacc accactttct acatggttgt
    53521 aggcattgtg ccgcgcgatg cgggcaacat cattattgat gatgacgata tcagtctgct
    53581 gcctctgcat gcacgcgcgc gccgcggtat cggctatctg ccacaggaag cctccatttt
    53641 ccgtcgcctc agcgtttacg ataacctgat ggcggtactg caaattcgtg acgacttgtc
    53701 tgctgaacaa cgtgaagacc gcgcgaacga gctgatggaa gagtttcaca ttgagcacct
    53761 gcgtgacagc atggggcagt cactctccgg gggtgaacgt cgccgtgtag aaattgcccg
    53821 cgcactggct gcgaatccga aatttattct gctcgacgaa ccgtttgccg gggttgaccc
    53881 gatctcggtt atcgacatta aacgcatcat tgaacacctg cgcgacagcg gcctgggcgt
    53941 gctgatcact gaccacaacg tgcgtgaaac actggcggtt tgtgaacgcg cttatatcgt
    54001 cagtcagggg catttgatcg cccacggcac gcctacagaa atcttacaag acgaacacgt
    54061 taagcgtgta taccttgggg aagacttcag actctgatag ggtagaagtt tgcgacgttt
    54121 tagcaggaga gtacgattct gaacatgaag caaggtttgc aactcaggct tagccaacaa
    54181 ctggcgatga cgccacagct ccaacaggca attcgtctgt tgcagttgtc gacgctggaa
    54241 cttcagcagg agctacagca ggcgctggag agtaatccgc tgcttgagca aatcgacact
    54301 catgaagaaa tcgacacccg cgaaacgcaa gacagtgaaa cgctggacac cgccgacgcg
    54361 ctcgaacaaa aagagatgcc ggaagagctg ccgctcgatg ccagttggga caccatttac
    54421 accgctggta caccatccgg caccagcggt gactacattg acgacgagct gccggtctac
    54481 cagggcgaaa cgacgcagac cttgcaggat tacctgatgt ggcaggtcga gctgacaccg
    54541 ttttccgaca ctgaccgcgc tattgctacc tctatcgtcg atgccgttga tgaaaccggt
    54601 tatctgactg tcccgctgga agatattctc gaaagtatgg gcgatgaaga gatcgacatc
    54661 gacgaggttg aagccgtcct taagcggatc caacggtttg acccggtcgg tgtagcggca
    54721 aaagatctgc gtgactgcct gctaatccaa ctctcccaat tcgataagac cacgccgtgg
    54781 ctggaagagg ccagattgat cattagcgat catctcgatc tgttagccaa tcacgacttc
    54841 cgcactttaa tgcgcgtcac gcgtctgaaa gaagatgtgc tgaaagaagc cgtcaatctg
    54901 atccagtcgc tcgatccgcg ccccgggcag tcgatccaga ctggcgaacc tgagtatgtc
    54961 attccagatg tgctggtgcg taagcataac ggtcactgga cggtagaact caacagtgac
    55021 agcattccgc gtctgcaaat caaccagcac tacgcctcga tgtgcaataa cgcgcgcaac
    55081 gatggtgaca gccagtttat ccgcagcaat ctgcaggatg ccaaatggtt gatcaagagt
    55141 ctggaaagcc gtaacgatac gctactgcgc gtgagtcgct gtatcgttga acagcagcaa
    55201 gccttctttg agcaaggtga agaatatatg aaaccgatgg tactggccga tatcgcccag
    55261 gctgtcgaaa tgcatgaatc gacgatatct cgcgtgacca cgcaaaaata cctgcatagt
    55321 ccacgaggca tttttgaact gaagtatttc ttttccagtc acgtcaatac cgagggcggc
    55381 ggcgaagctt cctccacggc gattcgtgca ctggtgaaga aattaatcgc ggcggaaaac
    55441 ccagcgaaac cgttgagcga cagcaagtta acctctttgc tgtcggaaca aggtatcatg
    55501 gtggcacgcc gcactgttgc gaagtaccga gagtctttat ccattccgcc gtcaaaccag
    55561 cgtaaacagc tcgtttgacc caaccgataa ggaagacact atgcagctca acattaccgg
    55621 aaataacgtc gagatcaccg aggcactgcg cgaatttgtt acagctaaat ttgccaaact
    55681 tgagcaatat tttgaccgaa tcaaccaggt ctatgttgtt ctgaaagtgg agaaagtcac
    55741 ccacacctca gatgcaacac tgcatgtaaa cggtggtgaa attcatgcca gcgcggaagg
    55801 tcaggacatg tacgctgcca ttgatggctt aattgataag ctggcacgcc agttgaccaa
    55861 acataaagat aaactgaaac aacactaatt gtccgggcaa ttagcatgtg catggcggcc
    55921 tgttgtgcgg cacaacgggc catttgtacg gttaatgctc cgagcctgtt ccactgtttg
    55981 agtgggcagg ttcttaggtg aaattatgac aaataatgat acaactctac agcttagcag
    56041 tgttcttaac agggaatgta cgcgaagccg cgtccactgt cagagcaaaa aacgcgccct
    56101 ggaaatcatc agcgagctgg cggcgaaaca acttagcctg ccgcctcagg tggtttttga
    56161 agctatcctg acgcgggaaa aaatgggcag taccggtatc ggcaatggta ttgccattcc
    56221 gcatggcaaa cttgaagaag atactctgcg tgccgttggc gttttcgttc agctcgaaac
    56281 gcctatagct ttcgatgcca tcgacaacca accggtggac ttactttttg ccctgctggt
    56341 gccggcagac caaactaaaa cgcacctgca tactctgtcg ctggtggcga aacgtctggc
    56401 ggacaaaacc atctgccgcc gtttacgcgc agcccagagc gatgaagagc tgtatcaaat
    56461 cattacggat accgaaggta ctccggatga agcgtagtta ttcggtaatg tctcttttag
    56521 acgttgtgag gagaaacagt acatggtact gatgatcgtc agcggacgtt caggttcagg
    56581 taaatctgtc gccctgcgtg cgctggaaga tatgggtttt tactgcgtgg ataaccttcc
    56641 cgtagtgttg ttacccgatc tggctcgaac tctggccgat cgcgagattt ctgccgccgt
    56701 cagcattgat gttcgtaata tgccggagtc accagaaata ttcgaacagg cgatgagtaa
    56761 cctgcctgac gctttctcac cgcaactact gttcctggat gccgaccgta ataccttaat
    56821 tcgtcgttac agtgacacgc gccgactgca tccgctttcc agcaaaaacc tgtcgctgga
    56881 aagtgctatc gacaaagaaa gcgatttgct ggagcctctg cgttcgcgag cggatctgat
    56941 tgtcgatacc tcagaaatgt ccgttcacga gctggctgaa atgctgcgta cccgtctgct
    57001 gggtaaacgt gaacgcgaac tgaccatggt ctttgagtct ttcggcttca aacacggtat
    57061 ccctatcgat gcagattacg tctttgacgt gcgcttcttg ccgaacccgc actgggatcc
    57121 gaaactgcgt ccaatgacag gtcttgataa acctgtcgcc gcgttcctcg accgccacac
    57181 agaagtacac aattttatct accagacgcg aagctatctt gagctatggt tacctatgct
    57241 ggaaaccaac aaccgtagct acctgacggt cgccattggt tgtaccggcg ggaagcaccg
    57301 ttcggtgtat attgcagagc aactggcaga ctacttccgc tcgcgcggta aaaacgtcca
    57361 gtcacgccat cgtacgctgg aaaaacgtaa accatgaccg tcaagcaaac tgttgaaatc
    57421 acaaacaagc tgggcatgca tgcccggcct gcaatgaagc tgtttgaatt aatgcagggt
    57481 tttgacgctg aagtgctgtt acgtaatgac gaaggcaccg aggctgaagc caacagcgtt
    57541 attgcgctgc tgatgttgga ttctgccaaa ggacggcaga ttgaagttga agcgaccggt
    57601 ccacaggaag aggaagcact ggccgccgtt atcgccctct ttaattctgg ttttgatgaa
    57661 gattaatctt catcactttg acatacaact cccttcaagc tcccccgaca ataagaaaat
    57721 cacgtactga aatcgttctc aatcaacgtc atttgtacat tttgtgcgct tttcactttc
    57781 agaagaacct taagaaaacc ttaagaggca ttgtttaggt tttgtttaag ttaatcgacc
    57841 atactggaga tcgtcagaaa atatttccag gagatggcat gattcgctta tctgaacaaa
    57901 gtcccctggg caccggaaga catcggaaat gttatgcgca tccggaagat gcccaacgct
    57961 gtatcaagat tgtctaccat cgtggcgatg gtggcgataa agagatccgc cgtgagttaa
    58021 agtactacgc gcatcttggt cgccggttaa aagactggag tggaataccg cgctatcacg
    58081 gtaccgttga gacggattgc ggaaccggtt atgtctacga tgtgattgcc gattttgacg
    58141 gcaagccttc cattacgctg accgaatttg ctgaacaatg ccgttacgaa gaagacattg
    58201 cacaactgcg ccagctactg aaacagctga agcgttattt gcaggataac cgtatcgtga
    58261 cgatgtcgct gaagccgcag aatatcctct gtcaccgcat cagtgagtca gaggttatcc
    58321 cggtcgtctg cgacaacatt ggtgaaagca cattgattcc actggcgacc tggtcgaagt
    58381 ggtgttgtct gcgtaagcaa gagcggttat ggaaacgatt tatcgcccaa ccggcgctgg
    58441 ctattgccct gcagaaagat ctgcaacctc gtgagagcaa aacgctggcc ctcacttcgc
    58501 gcgaagctta atccagctgg tgttgttgca taaacggctc accgcctaac tgatacatct
    58561 gccgtaaaat ccacgcctga cggctacgca cgtagcccga tggcgaggag actttgaaac
    58621 gaagtggatt aggtaataca gctgccagta atgcagcttc cgaccgggta agtttgctcg
    58681 cgggtttgtg gaaataacgt tgtgccgcag cttcgacgcc aaacacaccg tcgccaaatt
    58741 cggcgatatt caggtaaacc gtcaggatac gctttttgct ccagactgtt tctatcccca
    58801 gcgttaatcc agcttccagc ccttttcgta cccagctacg cccatcccat aaaaagagat
    58861 tttggccgtc tgctgagaaa tcgttgaagc accgcgaata cggttttcat tgcgctcgtt
    58921 gtgcgccagg gctttctcaa tggaagcgac atcaaagccc cagtgctcag gaaatttctg
    58981 atcttctgcg gcaatcaccg ccagtcccat ccacggcgag atttgatcca tactgaccca
    59041 gtcagaatgt gccacgtaac gaaaattgcc atgcagccag gcgctgacct gtcgctcgac
    59101 cattaccgct gagaagggaa caggcgcaac gctaaacaac gcgatgcccc cgccccagaa
    59161 aacggcgagg acaaccatta accgcaaaag aaaacggcga acgaaactaa acaccgttaa
    59221 gcggctttta ctcattcagc cagaaccagc acgcgggaaa ccagcttatc aatgccgctc
    59281 gccgcttctg caatgttctg cgccagcata tatgctgggg tggtgacaat cttattgtct
    59341 tcatcaacca cgatatcatc gacaggacac ggcacatgct ccgcgcccat ctcttccagc
    59401 acttctgcgg tatcgatatc agtaccgatg gtcaaacgca gcgggaaatc gaaaattttc
    59461 ggcagcatcg ccggggcaat acacataaaa ccaagcggtt ttccggcctg atgcatcgct
    59521 tgtgccagcg cctttaattc acggtcaacg gtgcattcgc taccaagact ggcaaaattg
    59581 cttaaattct tcgccgcgcc aaaccccccc ggcacaatca acgcatccag ttcagcggca
    59641 tcggcctggg ccagaggacg gatttcaccg cgcgttattc gtgccgcttc aatcagcaca
    59701 ttgcgcgttt ccgtcatcgc ttcgccagtt aaatggttga taacatcaac ctgctgctta
    59761 tccggtgcaa agcagaccgc ctgcgcaccg ctgcgtgaaa tagctaacaa cgtcaacacc
    59821 gcttcatgaa tttcagaacc gtcatagacg ccgcatccgc tcagaattac gccaattttc
    59881 ttcattgtga tcatcctttc gcaattgact gaaacacatt aattttttta ataaaaatgg
    59941 tacgcatcac acatttaact gattcatgta acaaatcatt taagttttgc tatcttaact
    60001 gcgtgcggcc tgaaaaacag tgctgtgccc ttgtaactca tcataataat ttacggcgca
    60061 gccaagattt ccctggtgtt ggcgcagtat tcgcgcaccc cggtctagcc ggggtcattt
    60121 tttagtggct tttgccaccc acgctttcag cacttctacg tcgtgacgcc actcttcttt
    60181 catctcttca atccattcac cgacgttatc ttcccaggcg ggaaggtcag gagactgaat
    60241 ttgctgaccc agctgttgca gatggcgtaa ccccactgac cccgccgcac ctttaatttt
    60301 atgtccttcc tcaacaatgc cttttttatc ctgcgccgtc agattcgact ccagcacgct
    60361 gacatagccc ggcatcatct tctcaaacac cgctaacccg tcggtgatca gcttcggtcc
    60421 tacaagttcg agatactgtt ccagcatggg aatatcgagc aatgcttctg atttactgtt
    60481 ctcttctgtc gtcaccgtac tctcctcatc atcctgggta tcccagaatt tcttgatcat
    60541 cgcggttagc gccggaaccg aaagcggctt actcagcaca tcatccattc cagcattgag
    60601 gtactcttgt ttgtctttca gcacgttagc ggttaaggcc accagcggcg gtaaatcctc
    60661 gcgcggataa cgcttcgtca gttcacgaga gatatccagc ccggtcatat ctggcaactg
    60721 aatatccagc aacaccaggt cgtattcgcc cggtttaaac atctccagcg ccgccttgcc
    60781 ggtcatggcg acatcaacgc tgttacctaa tttttccagc acagaacgcg cgacaatcac
    60841 gttcagttca atgtcttcca ccagcagcac attcagcgcc ggtaaaggca tatcgtcttc
    60901 atcaaacgca tcatcgacct cttctgccac cgacggtgcg tggatcgtca acgtaaaggt
    60961 tgaacctttg ccctgttcac tggtaaccgt aatatcgccg cccatatttt tcgccagacg
    61021 acgagaaacg gccagaccaa taccggtgcc ggtggcaggt ttaccgccat gactgtcttt
    61081 cacctggtaa tacatggcga agattttatc cagctcatcc tgcggaatgc cgataccaga
    61141 atcttccact tcaaaatgca gcatatcgcc ttcatcgtag cgcacgcgca cggtaacctg
    61201 gccttgctgg gtgaatttga cggcgttact gatgaggttc cacaggatct gccgtaaacg
    61261 cgtcccgtcg gtaatgacct gatgcggtaa tggcagcgta ggctccaggt taaagcgcaa
    61321 tcctttttgt tgcgcctgca atgcggagag attttccaga tcggcaagga agctggtgaa
    61381 atcaaccggc tgattatcaa gctggacctt gcgccgttcc atcttatcca tgtcgataat
    61441 atcgttaaag atattcccca gcgtgacggc cgaaacatgg atagttttga gatatttttc
    61501 ctgctcggcg gtgagttcgg tatccagcag aatgcggctc aggccgacga taccgttcag
    61561 cggtgtacgc aattcgtgac tgatggtgga aataaacgtc gttttgtcgc ggctggcgcg
    61621 ttcaagcgca tcctgatacc gcttacgctc ggtaatgtcg cgaccaaagc ccatcaaacc
    61681 gtgacgttta cccacgcggt cgtagtacgg cactttacgg atttcaaagc aggctttgcg
    61741 cccgtccggg taatccagcc actgttcata ggtcagtgac acattatgac ggaacacttt
    61801 ttcatcggtt tcaatgactt ttgcggcggc ttccggtgag taaacatcag caggtttcag
    61861 gtgaaccagt tgtttttcgc tttttccggt cagcagctcc atcgcgcggt tacagccgga
    61921 aaactcttta tcttcgttac gataaaaaac caagtcgggt gaagcatcaa ggaaggaacg
    61981 taagaatgag gattgctgct cgagctgaat ttgtgtctct tcgcgctctt tgatttcaat
    62041 tttcagttgg ccgaaggttt cctgcagttc tgcttccgct ttttcacgaa cggcaatttc
    62101 ctgatttagc tgggcaatat tatcttttaa ctgaacgttg aggctcaaat cgcgctcgcg
    62161 catctcctcc agtttttgca ccagccgtga cagacgttgt cgtgactcct ccagttgctc
    62221 gacgaccacc gatagaaagt agaccgccca cggcgtaatc agcaaaccaa agaagataga
    62281 acgaataaca tcaatgcttt cgacctgacc atgcagcacc atggttaccg ccatttgtac
    62341 cacaatggca agaacgacga gggccagcgc cagcaacatt gagaagcgca ccagacctaa
    62401 cttcatcatc aggtcaacat aatactgcgc cagcagacga atttgcttca ttagggaatt
    62461 ccttcacgac aacctgcccc aataataccc aattctgaca gttacgttat aaattgtgtg
    62521 agaaatgcgg aattcccctc aacgacctac tccgttggag gtagccacgg acgtcccagc
    62581 gccgatccct gtacgccatg ttcattcaga tagcgatcca gctcgaccat tcccgtccat
    62641 cgattttcgc accacagcgg agcaagcagc gtcgggcgac gggcgctggc ggaaatgcgg
    62701 tggtagatca cttccggtgg cgtatggcga atcatttctc cggcagtgag cgtataatcc
    62761 tccagttcaa taccgttcaa acgccccgct tcccaggctt tcgccataat gctgcctttc
    62821 acaatatgca gcggatgcag ctttatgccg tccacgctgg tttcaaccac tcgctccagc
    62881 gtttgcaaac attcagcctg cccttcgcca ggcaggccga caattaagtg ggaacacacc
    62941 ttcagcccgc gctgacgtgc cagctgggtt gtacgctgat aacaggcaaa atcatggccg
    63001 cggttgatgc gatgcagtgt tttgtcgtgg gcggtttgta gccccagctc cagccacact
    63061 tcgtaaccct ggtccttata ttcgcaaagc agatccagca ccgcatccgg cacgcagtcc
    63121 gggcgggtac caacacacaa accgacaata ttggcctggc tcaccgcctg ctgatacata
    63181 gaacgcagca cctgaacttc cgcaaaggtg ctggtatacg cctgaaagta ggccagatag
    63241 cgtttagcgc ggttaactaa attcgcctgg tgcgccagtt gctcggcaat ggaacgatgc
    63301 tgctgcgctt catcggcaaa cgaggcaaca ttacagaatg tgcagccacc acgcccgatg
    63361 gtaccgtcac ggttagggca gctaaaaccg ccgtgcagcg tcagcttatg caccttttgc
    63421 ccataacgac gggtgagatc accaccaaac atattgacta atttctgtaa ctgcataatc
    63481 tgatagaccg cgccttgaaa agaggccaaa gcctgccatt tttagctcaa ttcggcgatg
    63541 acctggatca atcgtcccgg cctgctttta tcaactgcat aatcaatcaa aattaccgaa
    63601 atttcatgca caatcacata aatcactttt gcttatcttg tgtcagattt ttttatctcc
    63661 ttatggattt taggcaaaaa cagtagcatg aaacgtcatt accaattaag gcagtataaa
    63721 atgctggttt tctcgtcagt tcaaggcagg ataagggtta acacgccttt atgacagtca
    63781 ggaattgact gtttctctaa cgactctcct tttagcctta aagataaaat ctattttaat
    63841 ttcagctatt taataaagaa ttttgcgcta aagcacattt ctgtaccaat aagcttgcca
    63901 tttgacctgt atcagctttc ccgataagtt ggaaatccgc tggaagcttt ctggatgagc
    63961 agcctgctca tcatatttat gcagtaattg agatcccctc ttcaccgtat taaccgatgc
    64021 gaaaaggaca acaagggggc gaatgcgagg cgcgcgtatg acacgcaaac cccgtcgcca
    64081 cgctctttct gtgcccgtgc gcagcggttc ggaagtgggg ttcccgcaga gcctggggga
    64141 ggttcacgat atgttgtacg ataaatccct tgagagggat aactgtggtt tcggcctgat
    64201 cgcccacata gaaggcgaac ctagccacaa ggtagtgcgt actgcaatac acgcactggc
    64261 ccgcatgcag caccgtggcg cgattctcgc cgatggtaaa accggcgacg gttgcggctt
    64321 gctgttacaa aaaccggatc gcttttttcg catcgttgcg caggagcgcg gctggcgttt
    64381 agcaaaaaac tacgctgtcg ggatgctctt cctgaataaa gatcctgaac tcgccgctgc
    64441 cgcacgccgc atcgttgaag aagaactgca acgcgaaacc ttgtcgattg tgggctggcg
    64501 tgatgtcccc actaacgaag gcgtgctggg tgaaatcgcc ctctcctctc tgccacgcat
    64561 tgagcaaatt tttgtgaacg ccccggcagg ctggcgtcca cgcgatatgg agcgccgtct
    64621 gtttatcgcc cgccgccgca ttgaaaagcg tctcgaagcc gacaaagact tctacgtctg
    64681 tagcctgtcg aatctggtga acatctataa aggtctgtgt atgccggcgg atctgccgcg
    64741 cttttatctg gatcttgcgg acctgcgtct ggaatcggcc atttgcctgt tccaccagcg
    64801 cttctccact aacaccgtac cgcgctggcc gctggcgcaa ccgttccgct atctggcgca
    64861 taacggtgaa atcaacacca tcaccggtaa ccgccaatgg gcgcgtgcgc gtacctataa
    64921 attccagacg ccgctgatcc ctgacctgca cgacgccgcg ccattcgtca acgaaaccgg
    64981 ttctgactcc agttccatgg ataacatgct ggaactgctg ctggcaggcg ggatggatat
    65041 catccgcgcc atgcgcctgt tagtgccacc cgcctggcag aacaacccgg atatggaccc
    65101 ggaactgcgt gccttctttg actttaactc catgcatatg gagccgtggg atggtccggc
    65161 gggcatcgtg atgtccgatg gtcgcttcgc tgcctgtaac ctcgaccgta acggtctgcg
    65221 tccggcacgc tacgtcatta ccaaagacaa gctcatcacc tgcgcctctg aagtcggtat
    65281 ctgggattac cagcctgacg aagtggtcga aaaaggccgc gtcgggccag gcgaactgat
    65341 ggtgatcgac actcgcagtg ggcgtattct gcactcggca gaaaccgatg acgatctgaa
    65401 aagccgccac ccttataaag agtggatgga gaaaaacgtc cgccgactgg taccgtttga
    65461 agatctgccc gatgaagaag tgggtagccg cgaactggac gacgacacgc ttgccagcta
    65521 ccagaaacag tttaactaca gcgcggaaga gctggactcc gtaattcgcg tactgggcga
    65581 aaacggtcag gaagcggtcg gttcgatggg cgatgatacc ccattcgccg tgctctccag
    65641 tcagccgcgc attatttacg actacttccg ccagcagttt gcccaggtga ctaacccgcc
    65701 aatcgacccg ctgcgtgaag cgcatgttat gtcgctcgcc accagtatcg gtcgtgaaat
    65761 gaacgtcttt tgcgaagcag agggccaggc gcaccgttta agctttaaat cgccgattct
    65821 gctctactcc gatttcaaac agctcacgac gatgaaagag gagcactacc gcgcagatac
    65881 gctggatatc acctttgacg tcactaaaac cacgctcgaa gcgacagtca aagagctatg
    65941 cgacaaagcc gaaaaaatgg tacgtagcgg caccgtgttg ctggtgctct ccgaccggaa
    66001 tatcgctaaa gatcgcctgc cggttccggc tccgatggcg gttggcgcga tccagacccg
    66061 tctggtcgat caaagcctgc gttgcgatgc caacatcatc gtcgaaaccg ccagcgcccg
    66121 cgatccgcac cacttcgccg tgttgctggg tttcggcgcg acggctattt atccgtacct
    66181 tgcctatgaa acgctgggcc gcctggtaga cacccacgcg attgccaaag attatcgtac
    66241 cgtgatgctc aactaccgta acggcatcaa caaaggcttg tacaaaatca tgtccaaaat
    66301 gggcatctcc accatcgcct cttaccgctg ctcgaaactg tttgaagcgg tcggtctaca
    66361 cgatgatgta gtgggcctgt gcttccaggg cgcggtcagc cgcattggtg gggcaagctt
    66421 tgaagacttc cagcaggatc tgctgaatct gtcgaaacgt gcctggctgg cgcgcaagcc
    66481 gattagccag ggcggcctgc tgaaatacgt ccacggcggc gaataccacg cctacaaccc
    66541 ggacgtggtg cgcacgctgc aacaggcggt acaaagcggc gagtacagcg actatcagga
    66601 atacgcgaag ctggttaatg agcgtccggc aaccacgctg cgcgatctgc tggcaattac
    66661 gccgggtgaa aacgcggtca acattgctga tgttgaaccg gcaagcgaac tgtttaaacg
    66721 ctttgatacc gccgcgatgt ctatcggcgc gttaagcccg gaagcccacg aggcgctggc
    66781 ggaagcgatg aatagcatcg gcggtaactc gaactccggt gaaggcggcg aagacccggc
    66841 gcgctatggc actaacaaag tgtcgcgcat caagcaggtg gcttctggtc gcttcggcgt
    66901 tactccggcg tatctggtca atgccgacgt cattcagatt aaagtcgccc agggcgcgaa
    66961 gccgggcgaa ggtggtcagt tgccgggcga caaagtcact ccttacatcg ccaaactgcg
    67021 ctattcggtg cccggagtga cgctgatctc cccgccgccg caccacgata tctactctat
    67081 cgaagactta gcgcagctta ttttcgacct caagcaggtt aacccgaaag cgatgatctc
    67141 cgttaagctg gtgtccgaac caggcgttgg caccatcgcg actggcgtgg cgaaagctta
    67201 tgcggacttg atcaccatcg cgggctatga cggcggcacc ggcgcaagcc cgctgtcgtc
    67261 agtgaaatat gcaggctgcc cgtgggaact cggtctggtg gaaacccaac aggcgctggt
    67321 tgccaacggc ttgcgtcata aaattcgttt gcaggttgat ggcggcctga aaacgggtgt
    67381 cgatatcatc aaagcggcga ttctcggcgc agaaagcttc ggcttcggca ctggcccaat
    67441 ggtggcgctc ggctgtaaat atctgcgtat ttgccacctg aacaactgcg ccacgggtgt
    67501 agcaactcag gatgacaaac tgcgtaagaa tcactatcac ggcctgccgt tcaaggtgac
    67561 taattacttt gagtttatcg cccgtgaaac ccgcgagctg atggcacagc tgggagtaac
    67621 gcgtctggtg gatctgattg gtcgcaccga cctgctgaaa gagctggacg gtttcaccgc
    67681 caaacagcag aaactggcgc tgtcgaagct gctggagact gccgagccac atgcaggtaa
    67741 gtcactctac tgcactgaaa acaacccgcc gtttgataac ggcctactga acgcgcagtt
    67801 gctgcaacag gcgaaaccgt ttgtcgatga acgccagagc aaaaccttct ggttcgatat
    67861 ccgcaacacc gaccgttctg tcggcgcgtc gctttcaggc tatatcgccc agacgcacgg
    67921 cgatcagggg ctggcagccg atcctatcaa agcgtacttc aacggcaccg caggccagag
    67981 tttcggtgtg tggaacgcgg gcggcgtgga actgtacctg accggggatg ccaacgacta
    68041 tgtcggtaaa ggcatggcgg gcggcttaat cgccattcgt cctccggttg gttccgcctt
    68101 ccgcagccat gaagcaagca ttatcggcaa cacctgcctg tatggcgcga ccggtggtcg
    68161 tctgtatgcc gcaggccgcg cgggtgaacg tttcggcgtg cggaactccg gtgctatcac
    68221 cgtggtagaa ggcattggcg acaacggctg tgaatatatg acgggcggta ttgtctgtat
    68281 tctgggtaaa accggcgtta acttcggcgc gggcatgacc ggcggcttcg cttacgttct
    68341 cgatgaaagc ggtgatttcc gcaaacgcgt taacccggaa ctggtcgaag tcttaagcgt
    68401 cgacgatctg gcgatccatg aagagcatct gcgcggtctt atcaccgagc atgttcagca
    68461 taccggttct cagcgcggtg aagagattct ggcgaactgg tcaaccttcg ccacgaaatt
    68521 cgcgctggtt aaaccgaagt ccagtgatgt aaaagcactg ctgggtcacc gtagtcgtag
    68581 cgcagcagag ttgcgcgtgc aggcgcagta aggggtagca acaatgagtc agaatgttta
    68641 tcaatttatc gacctgcagc gcgttgatcc gccaaagaaa ccgctgaaga tccgtaaaat
    68701 tgagtttgtt gaaatttacg agccgttttc cgaaggccag gccaaagcgc aggctgaccg
    68761 ctgtctgtcg tgcggcaacc catactgcga gtggaaatgc ccggtacaca actacatccc
    68821 aaactggctg aagctcgcca acgaggggcg tatttttgaa gcggcagaac tgtcgcatca
    68881 gaccaacaca ctgccggaag tatgcggacg tgtctgcccg caagaccgtc tgtgcgaagg
    68941 ttcctgcacc ctgaacgatg agttcggtgc ggtgaccatc ggcaacattg agcgttatat
    69001 caatgataaa gcgttcgaaa tgggctggcg tccggatatg tccggcgtga agcaaaccgg
    69061 taaaaaagtg gcgattatcg gtgcaggccc ggcaggtctg gcgtgtgcgg atgtcctgac
    69121 gcgtaacggc gtaaaagccg ttgtcttcga ccgtcatcca gaaattggcg ggctgctgac
    69181 cttcggtatt ccggccttca agctggaaaa agaggtaatg acgcgtcgcc gtgaaatctt
    69241 caccggcatg ggtattgaat tcaaactcaa taccgaagtg ggccgcgacg tacagctgga
    69301 cgatctgctg agtgattacg atgccgtgtt ccttggcgtt gggacttatc agtcaatgcg
    69361 cggcgggctg gaaaacgaag acgccgatgg cgtgtacgca gcgctgccgt tccttatcgc
    69421 caacaccaaa cagttaatgg gctttggtga aacccgcgac gaaccgttcg tcagcatgga
    69481 aggcaaacgc gtggtagtac ttggcggtgg cgacactgcg atggactgcg tgcgcacgtc
    69541 tgtacgccag ggggcaaagc acgttacctg tgcctatcgt cgtgatgaag agaacatgcc
    69601 gggttcccgc cgcgaagtga aaaacgcgcg ggaagaaggc gtagagttca aattcaacgt
    69661 ccagccactg gggattgaag tgaacggtaa cggcaaagtc agcggcgtaa aaatggtacg
    69721 taccgaaatg ggcgaaccgg atgccaaagg ccgtcgccgc gcggagatcg ttgcaggttc
    69781 cgaacatatc gttccggcag atgcggtgat catggcgttt ggtttccgtc cacacaacat
    69841 ggaatggctg gcaaaacaca gcgtcgagct ggattcacaa ggccgcatca tcgctccgga
    69901 aggcaacgac aacgctttcc agaccagcaa cccgaaaatc tttgctggcg gcgatatcgt
    69961 ccgtggttcc gatctggtcg ttaccgccat cgccgaaggt cgcaaagcgg cagacggcat
    70021 tatgaactgg ctggaagttt aagctctgtc ccagcgccgc ctcctcagaa aagagtcggc
    70081 gctgaaattc tcctttcttt ctggaaatcg cctcgcataa tctgaagaaa accaatgtaa
    70141 tacgctcatc tggattgtta ccggtagatg cccggcgtat agctgattcc attattcaac
    70201 gcagcgccat ataaagggac agataatgga atcgctctca gagagaacct caacaggcta
    70261 ccagcaaatc cacaacggca ttattcatct ggtcgatagc gcccgaacgg aaacggtacg
    70321 tagcgttaac gcgctgatga ccgcgacgta ctgggaaatt ggccgacgga ttgtcgaatt
    70381 tgaacaaggt ggtgaggcca gagcagctta tggtgcacag ttgattgatc ggctatcgaa
    70441 ggatttaagt cgacggtata aacgtggggt ctctgcaaaa aacttacgcc aaatgaggct
    70501 tttttacctc ttttttcaac atgttgaaat tcgccagaca gtgtctggcg aattaacacc
    70561 attgggaatt ccgcagacac catctgcaga attcccatcg gcaaagattt ggcagacgct
    70621 gtctgccaaa ttgagaggct gtttgatatt tccacagctt ttgtattgca tctcctattt
    70681 tcggtttgta tattcatgca aataaacgaa gg
//
LOCUS       DAZPCS010000003        66990 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZPCS010000003 DAZPCS010000000
VERSION     DAZPCS010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66990
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.003"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            1..581
                     /locus_tag="ACHUQ7_000147"
                     /pseudo
     CDS             1..581
                     /locus_tag="ACHUQ7_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
     gene            684..1007
                     /locus_tag="ACHUQ7_000148"
     CDS             684..1007
                     /locus_tag="ACHUQ7_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 family protein"
                     /protein_id="HFR1133736.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLESLD
                     RSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNHAL
                     TVIVG"
     gene            complement(1074..2531)
                     /gene="nuoN"
                     /locus_tag="ACHUQ7_000149"
     CDS             complement(1074..2531)
                     /gene="nuoN"
                     /locus_tag="ACHUQ7_000149"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFR1133737.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2538..4067)
                     /gene="nuoM"
                     /locus_tag="ACHUQ7_000150"
     CDS             complement(2538..4067)
                     /gene="nuoM"
                     /locus_tag="ACHUQ7_000150"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFR1133738.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMTFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4231..6072)
                     /gene="nuoL"
                     /locus_tag="ACHUQ7_000151"
     CDS             complement(4231..6072)
                     /gene="nuoL"
                     /locus_tag="ACHUQ7_000151"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFR1133739.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFRDMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRSLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6069..6371)
                     /gene="nuoK"
                     /locus_tag="ACHUQ7_000152"
     CDS             complement(6069..6371)
                     /gene="nuoK"
                     /locus_tag="ACHUQ7_000152"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFR1133740.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6368..6922)
                     /gene="nuoJ"
                     /locus_tag="ACHUQ7_000153"
     CDS             complement(6368..6922)
                     /gene="nuoJ"
                     /locus_tag="ACHUQ7_000153"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFR1133741.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(6934..7476)
                     /gene="nuoI"
                     /locus_tag="ACHUQ7_000154"
     CDS             complement(6934..7476)
                     /gene="nuoI"
                     /locus_tag="ACHUQ7_000154"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFR1133742.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7491..8468)
                     /gene="nuoH"
                     /locus_tag="ACHUQ7_000155"
     CDS             complement(7491..8468)
                     /gene="nuoH"
                     /locus_tag="ACHUQ7_000155"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFR1133743.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDRVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8465..11191)
                     /gene="nuoG"
                     /locus_tag="ACHUQ7_000156"
     CDS             complement(8465..11191)
                     /gene="nuoG"
                     /locus_tag="ACHUQ7_000156"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFR1133744.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSSASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11244..12581)
                     /gene="nuoF"
                     /locus_tag="ACHUQ7_000157"
     CDS             complement(11244..12581)
                     /gene="nuoF"
                     /locus_tag="ACHUQ7_000157"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFR1133745.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12578..13078)
                     /gene="nuoE"
                     /locus_tag="ACHUQ7_000158"
     CDS             complement(12578..13078)
                     /gene="nuoE"
                     /locus_tag="ACHUQ7_000158"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFR1133746.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13081..14883)
                     /gene="nuoC"
                     /locus_tag="ACHUQ7_000159"
     CDS             complement(13081..14883)
                     /gene="nuoC"
                     /locus_tag="ACHUQ7_000159"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFR1133747.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(14977..15639)
                     /gene="nuoB"
                     /locus_tag="ACHUQ7_000160"
     CDS             complement(14977..15639)
                     /gene="nuoB"
                     /locus_tag="ACHUQ7_000160"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFR1133748.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15655..16098)
                     /gene="nuoA"
                     /locus_tag="ACHUQ7_000161"
     CDS             complement(15655..16098)
                     /gene="nuoA"
                     /locus_tag="ACHUQ7_000161"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFR1133749.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16729..17667)
                     /gene="lrhA"
                     /locus_tag="ACHUQ7_000162"
     CDS             complement(16729..17667)
                     /gene="lrhA"
                     /locus_tag="ACHUQ7_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFR1133750.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKVDIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            18586..19803
                     /gene="alaA"
                     /locus_tag="ACHUQ7_000163"
     CDS             18586..19803
                     /gene="alaA"
                     /locus_tag="ACHUQ7_000163"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFR1133751.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            19887..20486
                     /gene="yfbR"
                     /locus_tag="ACHUQ7_000164"
     CDS             19887..20486
                     /gene="yfbR"
                     /locus_tag="ACHUQ7_000164"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFR1133752.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNAQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(20545..22377)
                     /locus_tag="ACHUQ7_000165"
     CDS             complement(20545..22377)
                     /locus_tag="ACHUQ7_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFR1133753.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTSVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTNEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(22464..23114)
                     /gene="hxpA"
                     /locus_tag="ACHUQ7_000166"
     CDS             complement(22464..23114)
                     /gene="hxpA"
                     /locus_tag="ACHUQ7_000166"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HFR1133754.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(23125..23619)
                     /locus_tag="ACHUQ7_000167"
     CDS             complement(23125..23619)
                     /locus_tag="ACHUQ7_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFR1133755.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMDMYHALYVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(23702..24157)
                     /gene="yfbV"
                     /locus_tag="ACHUQ7_000168"
     CDS             complement(23702..24157)
                     /gene="yfbV"
                     /locus_tag="ACHUQ7_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFR1133756.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            24495..25697
                     /gene="ackA"
                     /locus_tag="ACHUQ7_000169"
     CDS             24495..25697
                     /gene="ackA"
                     /locus_tag="ACHUQ7_000169"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFR1133757.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVAFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            25772..27916
                     /gene="pta"
                     /locus_tag="ACHUQ7_000170"
     CDS             25772..27916
                     /gene="pta"
                     /locus_tag="ACHUQ7_000170"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFR1133758.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28106..29626
                     /gene="yfcC"
                     /locus_tag="ACHUQ7_000171"
     CDS             28106..29626
                     /gene="yfcC"
                     /locus_tag="ACHUQ7_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFR1133759.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(29659..30201)
                     /gene="yfcD"
                     /locus_tag="ACHUQ7_000172"
     CDS             complement(29659..30201)
                     /gene="yfcD"
                     /locus_tag="ACHUQ7_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFR1133760.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSGVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(30259..30810)
                     /gene="yfcE"
                     /locus_tag="ACHUQ7_000173"
     CDS             complement(30259..30810)
                     /gene="yfcE"
                     /locus_tag="ACHUQ7_000173"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFR1133761.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPAGYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(30866..31510)
                     /gene="yfcF"
                     /locus_tag="ACHUQ7_000174"
     CDS             complement(30866..31510)
                     /gene="yfcF"
                     /locus_tag="ACHUQ7_000174"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFR1133762.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            31646..32293
                     /gene="yfcG"
                     /locus_tag="ACHUQ7_000175"
     CDS             31646..32293
                     /gene="yfcG"
                     /locus_tag="ACHUQ7_000175"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFR1133763.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFEAAAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            32350..32712
                     /gene="folX"
                     /locus_tag="ACHUQ7_000176"
     CDS             32350..32712
                     /gene="folX"
                     /locus_tag="ACHUQ7_000176"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFR1133764.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            32733..33626
                     /locus_tag="ACHUQ7_000177"
     CDS             32733..33626
                     /locus_tag="ACHUQ7_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFR1133765.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(33674..34564)
                     /gene="rpnB"
                     /locus_tag="ACHUQ7_000178"
     CDS             complement(33674..34564)
                     /gene="rpnB"
                     /locus_tag="ACHUQ7_000178"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFR1133766.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFINIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(34761..35534)
                     /gene="hisP"
                     /locus_tag="ACHUQ7_000179"
     CDS             complement(34761..35534)
                     /gene="hisP"
                     /locus_tag="ACHUQ7_000179"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFR1133767.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(35542..36258)
                     /locus_tag="ACHUQ7_000180"
     CDS             complement(35542..36258)
                     /locus_tag="ACHUQ7_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1133768.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(36255..36941)
                     /gene="hisQ"
                     /locus_tag="ACHUQ7_000181"
     CDS             complement(36255..36941)
                     /gene="hisQ"
                     /locus_tag="ACHUQ7_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFR1133769.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(37031..37813)
                     /gene="hisJ"
                     /locus_tag="ACHUQ7_000182"
     CDS             complement(37031..37813)
                     /gene="hisJ"
                     /locus_tag="ACHUQ7_000182"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFR1133770.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(38034..38816)
                     /gene="argT"
                     /locus_tag="ACHUQ7_000183"
     CDS             complement(38034..38816)
                     /gene="argT"
                     /locus_tag="ACHUQ7_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFR1133771.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYALFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDYAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(39082..39651)
                     /gene="ubiX"
                     /locus_tag="ACHUQ7_000184"
     CDS             complement(39082..39651)
                     /gene="ubiX"
                     /locus_tag="ACHUQ7_000184"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFR1133772.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTLDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(39746..41263)
                     /gene="purF"
                     /locus_tag="ACHUQ7_000185"
     CDS             complement(39746..41263)
                     /gene="purF"
                     /locus_tag="ACHUQ7_000185"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFR1133773.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(41300..41788)
                     /gene="cvpA"
                     /locus_tag="ACHUQ7_000186"
     CDS             complement(41300..41788)
                     /gene="cvpA"
                     /locus_tag="ACHUQ7_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFR1133774.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIHGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(42047..42709)
                     /gene="dedD"
                     /locus_tag="ACHUQ7_000187"
     CDS             complement(42047..42709)
                     /gene="dedD"
                     /locus_tag="ACHUQ7_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFR1133775.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEVPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(42699..43967)
                     /gene="folC"
                     /locus_tag="ACHUQ7_000188"
     CDS             complement(42699..43967)
                     /gene="folC"
                     /locus_tag="ACHUQ7_000188"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFR1133776.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGLEKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(44037..44951)
                     /gene="accD"
                     /locus_tag="ACHUQ7_000189"
     CDS             complement(44037..44951)
                     /gene="accD"
                     /locus_tag="ACHUQ7_000189"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFR1133777.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(45107..45766)
                     /locus_tag="ACHUQ7_000190"
     CDS             complement(45107..45766)
                     /locus_tag="ACHUQ7_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR1133778.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(45849..46661)
                     /gene="truA"
                     /locus_tag="ACHUQ7_000191"
     CDS             complement(45849..46661)
                     /gene="truA"
                     /locus_tag="ACHUQ7_000191"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFR1133779.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(46661..47674)
                     /locus_tag="ACHUQ7_000192"
     CDS             complement(46661..47674)
                     /locus_tag="ACHUQ7_000192"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFR1133780.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(47740..48876)
                     /gene="pdxB"
                     /locus_tag="ACHUQ7_000193"
     CDS             complement(47740..48876)
                     /gene="pdxB"
                     /locus_tag="ACHUQ7_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFR1133781.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            48975..49970
                     /gene="flk"
                     /locus_tag="ACHUQ7_000194"
     CDS             48975..49970
                     /gene="flk"
                     /locus_tag="ACHUQ7_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFR1133782.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNHRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(49967..51145)
                     /locus_tag="ACHUQ7_000195"
     CDS             complement(49967..51145)
                     /locus_tag="ACHUQ7_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1133783.1"
                     /translation="MTAVSQTETRSSASFSLFRISFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(51400..52641)
                     /gene="fabB"
                     /locus_tag="ACHUQ7_000196"
     CDS             complement(51400..52641)
                     /gene="fabB"
                     /locus_tag="ACHUQ7_000196"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFR1133784.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNIKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKRLIRSRPE"
     gene            52800..54806
                     /gene="mnmC"
                     /locus_tag="ACHUQ7_000197"
     CDS             52800..54806
                     /gene="mnmC"
                     /locus_tag="ACHUQ7_000197"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFR1133785.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVIAESGFGTGLNFLTLWKAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDESRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(54927..55205)
                     /locus_tag="ACHUQ7_000198"
     CDS             complement(54927..55205)
                     /locus_tag="ACHUQ7_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFR1133786.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(55239..55787)
                     /gene="epmC"
                     /locus_tag="ACHUQ7_000199"
     CDS             complement(55239..55787)
                     /gene="epmC"
                     /locus_tag="ACHUQ7_000199"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFR1133787.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(55787..56596)
                     /locus_tag="ACHUQ7_000200"
     CDS             complement(55787..56596)
                     /locus_tag="ACHUQ7_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HFR1133788.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVMLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(56596..57420)
                     /gene="mepA"
                     /locus_tag="ACHUQ7_000201"
     CDS             complement(56596..57420)
                     /gene="mepA"
                     /locus_tag="ACHUQ7_000201"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFR1133789.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(57424..58509)
                     /gene="aroC"
                     /locus_tag="ACHUQ7_000202"
     CDS             complement(57424..58509)
                     /gene="aroC"
                     /locus_tag="ACHUQ7_000202"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFR1133790.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     FGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(58544..59476)
                     /gene="prmB"
                     /locus_tag="ACHUQ7_000203"
     CDS             complement(58544..59476)
                     /gene="prmB"
                     /locus_tag="ACHUQ7_000203"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFR1133791.1"
                     /translation="MDKIFVDEAVNELQTIQDILRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            59642..60193
                     /gene="smrB"
                     /locus_tag="ACHUQ7_000204"
     CDS             59642..60193
                     /gene="smrB"
                     /locus_tag="ACHUQ7_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFR1133792.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(60297..61187)
                     /locus_tag="ACHUQ7_000205"
     CDS             complement(60297..61187)
                     /locus_tag="ACHUQ7_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFR1133793.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASFDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(61174..61698)
                     /locus_tag="ACHUQ7_000206"
     CDS             complement(61174..61698)
                     /locus_tag="ACHUQ7_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1133794.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(61695..62165)
                     /locus_tag="ACHUQ7_000207"
     CDS             complement(61695..62165)
                     /locus_tag="ACHUQ7_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1133795.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDYQ"
     gene            complement(62162..62710)
                     /locus_tag="ACHUQ7_000208"
     CDS             complement(62162..62710)
                     /locus_tag="ACHUQ7_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1133796.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINRVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(62715..63437)
                     /locus_tag="ACHUQ7_000209"
                     /pseudo
     CDS             complement(62715..63437)
                     /locus_tag="ACHUQ7_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281619.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(63457..66097)
                     /gene="yfcU"
                     /locus_tag="ACHUQ7_000210"
                     /pseudo
     CDS             complement(63457..66097)
                     /gene="yfcU"
                     /locus_tag="ACHUQ7_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
     gene            complement(66179..66742)
                     /locus_tag="ACHUQ7_000211"
     CDS             complement(66179..66742)
                     /locus_tag="ACHUQ7_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1133797.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     NAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        16696 a        18147 c        16685 g        15462 t
ORIGIN      
        1 ccaaccctgg gtcactgcat tatcgccaac gccccgacac gtaagtcctg agcaattaga
       61 aaaaacagtt cgttatctta cacaaactgt gcatccacgt aatgccggca atattgataa
      121 tctgaatagg tccgcagagt atataaaaga gttctttgtc agtagcggtg ccagagttac
      181 cgcgcagggc gtccccgatt ggctattacg actctgcgcc tggaagccag aattaccctt
      241 acccggcaat gtcctggctt tatcccgatc ggggagactt tattgctgtg gtcggcagaa
      301 tgcaggatat caacgccgtt cgtcaggtaa aagcggcatt attgtcatcc cgggatttat
      361 ctgtttattc tatgaatgcc ccagggttta ttcccggtat tgatttttct gatcacctca
      421 attattggca acacgatatt cccgccgtaa tgattactga caccgctttt tatcgtaata
      481 aacaatacca cttgcccggt gataccgcag acagattgaa ttatcagaaa atggctcagg
      541 tggtagatgg tgttataacc ttgttataca acagtaaata ataaaaattt ctcgaggcgg
      601 gtccagaaac agcgacatgt acattttatt ttccctcgtc tgttttaaga tgcgaaacat
      661 aaaattcaat ggaggaaaaa taaatgggaa tgattggcta ttttgcagaa attgactcag
      721 aaaagattaa ccaactgctt gagtctactg aaaaaccttt gatggataat atacatgaca
      781 cgctttcagg attacgccga ctggaatcac tggatcgaag tgagctgcgt aaacagtttt
      841 ccattaagcg actcaatgaa atggagattt atccgggagt gaccttttca gaagagttag
      901 aggggcaact ctttgcctcg atcatgcttg atatggaaaa gctgattagc gcgtatcgac
      961 gtatgctgcg acaagggaat catgcgctga cggttattgt tgggtgatgg tgtcagagcg
     1021 ccaaaaaaaa ccgccgaaca tgtcggcggc tttctgactt acaaactaac agtttacatc
     1081 agcggcattg ctaaccgcac aatactaatc agcggttgtg gccatacacc cagcaccagt
     1141 accaacagtg cagaaatcag caccacgata ccgcccgcgc tgtactgcca gtttgacggt
     1201 gcatcgcgac ccggttgttc cggtgcgtgc aggtacaggc tcaccgccac gcgcaggtag
     1261 tagtagaggc cgattgccga accgacaacc acggcaccca ccagccacca caagtgtgcc
     1321 tggacaccga ccgccagcac gtagaactta ccgataaagc ccagcgtcat cgggataccg
     1381 gccagagaca gcatcatcac cgtcatcact gccgcgagga tcggacgatg ccagaacaga
     1441 ccgcggtaag agaacaggga atcagcatcc gggccacgat acgggctgga catcaggctg
     1501 accacgccga acgcgccgag gctgctgaac agataaccgg ccaggtaaac ccctaccgct
     1561 tccatcgaca tctcgccggt ttgcagcgca atcagcgcca ccagcagata gccgaggtga
     1621 gagatagatg agtaaccgag cagacgtttg atgttggtct ggctcaacgc catcaggtta
     1681 ccgaagatga tggaggcaaa ggcgataatc gccagtacta cgcgaatcgc ttcgctgtca
     1741 cccaccggtg cgtagaggaa cagacgcatc accacaccga agatagcgat tttgctcgcc
     1801 gtcgccagga aagtggaaac cggcgcaggc gcgccctggt atacgtctgg cgtccacagg
     1861 tggaacggca ccagagagag tttgaagccg aggccaacaa tcatcatgcc gaaacctgcc
     1921 agcaacagcg gctcgttgag cataccgtcg ccgaggtttt tacccagcgc gacaaacgac
     1981 aggtcgccag actgcgcata caccagcgcc ataccgaaca gcaggaaaga agacgctgcg
     2041 gcagaaagga tggtgtattt gatactggct tccagcgaac gtttctggcg gaaagcgtaa
     2101 ccgaccaggc caaacagcgg caaagagatc agttcaatac cgaggaacag agacgccaga
     2161 tggtttgcat tcgccagcag gatcccgccc agcgcggcaa ttaacaccaa caggtagaac
     2221 tcatccttgt tgtcgttata gccttcaagc cacgggtagg cgaaagtaca ggtggcgagg
     2281 ctcgccaaca ataccagccc ggtgtaaagc atggcgaaac catcaacgcg catcagcggc
     2341 gtaacgtcca tagcgcccgc ctggccaaca aaccagagcg aaaccagcgc cgcgttaagc
     2401 ccaataaccg agagcgtagc gttgaggaaa tgattgcgtc gccacgcaat ggagagcatc
     2461 acaaccacca ccgtcaagcc gacgatcagc aacggtagca gtgcgatcag gttttgtgga
     2521 gttattgtca tggcgattta cggccttgta gtagtaacgg aattaacaaa ccactgctgg
     2581 atattgccaa tcgcggagtg cgaggtatcc agaatcggct gcggatagaa gcccagcagt
     2641 accagcagca ccaccagcaa caggatcata aacagctcac gcagcgacat ccctggcagt
     2701 tcctggctgg caatctggct tttcgcttta ccgaagtaag cgcgatgcaa catcgccagc
     2761 gaataaacag atgcaaagac cagcccaaag gtagagataa cggtaatcac cgggacaacc
     2821 tggaagctgc cgaacagaat cataaattcg ccgacgaagt taccggtgcc aggcatccca
     2881 agcgttgcca ccgcaaagaa cagcgacagt gctggcagcc atttcatctt gctccacaga
     2941 ccgcccatca tgcgcatgtc gcgggtatgg atacgttcat aaagctgacc gcagagaata
     3001 aacagacccg ccgccgacaa gccgtgcgca atcatctgga ttaccgcgcc ctggtaggcc
     3061 aactggctgc cggtgtagat tgcaatcagc acgaagccca tgtgggaaac cgaggtgtag
     3121 gcgatcagac gtttgatatc ggtctgagcg aaggtcatcc acgcaccgta gaagatgccg
     3181 ataacaccca gccacatagc gattggcgcg aactctgccg acgcgttcgg gaacagcggc
     3241 agggagaaac gcaacaaacc gtaagcggcg gttttcagca agatccccgc gaggtccacg
     3301 gaaccggcgg tcggagcctg ggagtgcgca tccggcagcc agccatgcag cggaaccacc
     3361 ggcattttga ctgcgaaggc gatgaagaag cccagcatca acagatattc cacaccactg
     3421 gacattggcg tattcagcag ctcttcatag ttgaaggtcc agacgccggt cgcattgtag
     3481 tgaacaaaaa ccagcgccag gatggcgatc aacatcacca gaccactcgc ctgggtgtaa
     3541 atgaagaact tggttgccgc cgtgatacgc gttttaccgt cagaggcttt atgcccccac
     3601 agtgcgatca ggaagtacat cggcaccagc atcatttccc agaagaagaa gaacaggaac
     3661 atgtcgatgg caaggaacac gccgataacg ccgcccagga tccacatcag gttgaggtgg
     3721 aagaagccct gatatttttc gatctctttc cacgaacata gtaccgccag cacaccgagc
     3781 agaccggtca gcacgaccat cagcagcgac agaccgtcaa tggcgagatg aatagagata
     3841 ccaaaacgcg ggatccacgg catgtcgaat tcagactgcc actgcggaat tccggcggat
     3901 tgcgtcagtg aataaccgcc ctgcaaccac agttgcagcg acagcgccag cgtcaatccc
     3961 atggtgatca gcgcgatcca gcgtggcacc ttgacgccaa agcgttcggt ctgccagcac
     4021 aggaagccgc caataaaggg aattaatatt agccagggta gtaacatggc gatctttatt
     4081 ccttgtaaaa gtcccgtcag gaccggattt tcaacgaatt ctcacgacag aaaattctct
     4141 tcggactggg ggtagtgtcg gatgcgccgc gaaggcagct tatccgacct acgttcttat
     4201 atgcccaggg gcaatcccac aatccttaac tcaacgcagt accatcaaca gtgccagcac
     4261 cacgaccgca ccgatgctca tggatgccac ataccagcgc agataaccgt tctcgcttaa
     4321 cagcagacct ttacctgcaa agcgggaaag aaccgccggg atgttcatca ttgagttcag
     4381 cggatcgcgt ttcagcaacc aggcaatacc caggaacggc ttgacgaaca ctttgtcgta
     4441 cagccagtca aagccccaag cgttgtacca ccaggtactc agcaggcggc ccggcgcact
     4501 gttggcgatg gaggtcacca gagaacgttt acccagccac agccaggctg ccagcagaat
     4561 gccgaccacc gcgaccacgc cagaggtaat ttccagggtc aacatgctgc cgtgcgccag
     4621 ttccgtcgtt tgcggaagca cgccctgcag cggcggtaca atcagtgcgc caacgaaggt
     4681 ggaaaggatc agcagcacaa tcagcggcag gctgtgagtt acccctttca cggcgtgagc
     4741 gtgaatttgt tcttttccgt ggaagacgat gaaaatcata cggaaggtgt agagcgaggt
     4801 cataaacgca ccgaccagac ctgccaccat cagattgata tgaccattcg ccatcgcacc
     4861 cgcgaggatc tcatccttac tgaagaagcc cgcagtgacc agcggcagtg ccgacagtgc
     4921 tgcgccgccc accaggaagc agagataaac cagcggaata gatttacgca gaccgcccat
     4981 cttgaagatg ttctgttcgt gatggcaggc cagaatgacg gaaccggatg ccaggaacag
     5041 cagcgcttta aagaacgcgt gagtcatcaa gtggaaaatc gccgcatccc atgcctgcac
     5101 gccaagcgcg aggaacatgt agccaatctg gctcatggta gagtaagcga gaacacgttt
     5161 gatgtcggtc tgtaccagcg cggcaaaacc ggccagcagc agcgtaaccg ccccgacaat
     5221 acccaccaga tgcagaactt ccggcgtcat caggaacagg ccgtgggtac gggcgatcag
     5281 gtagacaccc gcggttacca tcgttgcggc gtggatcagc gcggagacag gcgtcgggcc
     5341 cgccatcgcg tcagcaagcc atgtctgcaa cggcaactgc gcagatttac cgaccgcacc
     5401 gcccagcagc atcagcgtcg cccacatcag catgttattg ccgtcagcaa agtgcgctgg
     5461 tgccagttcc accatatcgc ggaagttcag ggtgcccagt tcgttgtaaa gaatgaacag
     5521 tgcgaaagcg aggaaaacgt cacccacacg ggtcacgacg aacgctttca ttgctgctgc
     5581 gccattcttc ggatcggtgt aatagaaacc gatcagcaga taggagcaca ggcccacgcc
     5641 ttcccagccg aggtacatca gcagcaggtt atcggcgaga accagaacta ccatgctggc
     5701 gatgaacagg ttggtgtagg cgaagaagcg agagtagccc tcttcaccgc gcatgtacca
     5761 ggaggcgtac atgtgaataa ggaaacccac accagtgacc accgagagca tggtcagcga
     5821 caggccgtcc agcaccaggt taaaaccgat gttaaagtcg cctaccgaca tccacgtcca
     5881 cagcggctgg ctgtatgcct gctcgccgtt agcgaagaaa tcaacgccga taaaggcggt
     5941 taccagcgcc gccaggccca cagagccaac gccgacgatc gccgaaacgt tttcagacca
     6001 gcgcccacgg gagaacgcca gcaggacgaa gccaatcaat ggcaaaataa tggttaaggc
     6061 aagcatgttc atccgcgcat ctcacttact gaatcgatgt tcaggttctg gcgacgacgg
     6121 tgaagttgca gcagcagcgc aaggccgata ctcgcttctg ccgccgcgag gctgatggcg
     6181 agaatgtaca tcacctgacc gtcggtctga ccccagtagc ttccggcgac cacaaaggcc
     6241 agcgcggagg cgttaatcat gatttccaga ccaatcagca taaacagcag attgcgacgg
     6301 ataaccagac cggttaagcc aagaacgaat aagattgccg cgaggatcag tccatgttgt
     6361 aaggggatca tgcgtgctcc tccgtttttc ttttcgcgct gtcgtcttta cgattgctca
     6421 gcacttcacc cgcacgctct tcacgaccga cgtggaaggc cacaaccaga cccgcgagca
     6481 gcagcataga agccagttcc accgccagta cgtaaggccc gaacagcgta atacccactg
     6541 ctttagcact gattggcgta ccgtcgatac cctgatcgtt aacaccgagg atggcgtaaa
     6601 caatcaccac cagcatgatg gccgacaaaa ttgccgggcc aatccacacc tgcggtttca
     6661 gccactggcg ttcctgttcg atttctgaac cgcccaggtt gagcatcatc accacgaaca
     6721 cgaacagcac cataatggca cccgcgtaga cgataatttc cagcgcaccg gcgaagtaag
     6781 cgcctagtga gaagaacacc ccggaaatcg ccagcagcga aataatcagg tacagcagtg
     6841 cgtgtaccgg attggtatgg gtgatcactc gcaaggtcgc aagtatggct atcaggccac
     6901 agatataaaa agcgaactcc attgcccctc tccttacggt aacaggctct tgacgtcgat
     6961 aggcttggct tcgttctctg cttcgccctt atctttgccg tcgattgcca tacctgccat
     7021 ccggtagaag ttatattccg ggtatttgcc cggaccggag atcagcagat cctctttctc
     7081 gtaaaccaga tcctggcgct tgtattcccc catttcgaaa tccggggtta actgaatcgc
     7141 cgtggtcgga caggcttctt cgcacagacc acagaaaatg cagcgtgaga agttaatgcg
     7201 gaaaaattcc ggataccagc gaccgtcttt ggtttctgct ttttgcagcg agatacagcc
     7261 gaccgggcag gctaccgcac agaggttaca ggctacgcaa cgctcttcgc cgtccgggtc
     7321 gcgggtcaga acgatacgac cacgataacg gggcggcaga tagaccggct cttccgggta
     7381 cattcgcgtt tcgcgtttgg cgaacgcgtg caggccgatc atccagatac tacgaacctg
     7441 ggtgccgaaa cctactaaca attcttttaa ggtcatggtc ttattgcccc ttattgcgcc
     7501 tgccagagaa tgacagccgc cgttaccagc aagttgatca gcgtcagcgg caggcagatt
     7561 ttccagccga aggacattac ccggtcataa cgcggacgcg gtaacgacgc acgaatcaaa
     7621 atgaacatca tcataaagaa cgcggttttc agcgcgaacc agatgaatgg cggtaacaac
     7681 gggccttgcc agccaccgaa gaacagcgtc accatcaatg cagagatggt cacaatcccg
     7741 atgtactcac ccacgaagaa cagaccgaac ttcataccgg aatattcaat gtggtaacca
     7801 tccgccagtt cctgctcggc ttccggctgg tcaaacgggt gacggtgaca caccgccacg
     7861 cccgcgatgg caaaggtaat aaaaccaaag aattgcggga tgacgttcca cacatgcgcc
     7921 tggctgttga cgatgtcggt catgttgaat gaaccggcct gcgccaccac gcccatcagg
     7981 gaaagcccga ggaacacttc gtagctcagg gtctgcgcag aagcacgcat cgcacccagc
     8041 agcgagtatt tgttgttact tgaccagccc gcaaacagca ccgcgtaaac cgccagacct
     8101 gccatcatca ggaagaacaa aatcccgatg ttcaggtcgg caaccaccca acccggactg
     8161 actggcacaa ttgcaaaggc cagcagcagc gaggtaaagg caatcatcgg tgccagggta
     8221 aagatgacgc gatccgagaa tttcgggatc cagtcttctt taaagaacat tttgatcatg
     8281 tccgcaacca gctggagcga accgccccag ccaacacggt taggtccata acggttctgg
     8341 aacagaccca gcaggcgacg ttcgccaaag ctcatgaatg ccccacaggt gacaaccacc
     8401 agcaggatca ccaccgcttt gaggatggtc agcaggatct caatcagttc cggtgatatc
     8461 caactcattg ttgtgcctcc ttgagatcct caagatgcgc gccagccagc accggagcaa
     8521 tgccggacat acccatcggc aagcccacct gccctgccgt cagtccttcg gcgatttcaa
     8581 ccggcagcgt gaccgtgttg ccatcgtaac taaaggagac gcgtgtacct gcgttcacac
     8641 ccaacttcgc ggcatccgct gggttgagtt tgatgtacgg ctgcggcata cggctctgga
     8701 agaccggagc acgctgtgac aattcatcgc tgccaaacag gtggtagtac ggcgcgatac
     8761 gccatttccc gtcctgcggc tggaagcgtg ccggtacgct ggtgaagtaa tccagaccgt
     8821 tttcgctggt ttcaaacaga cgcacgcccg gatcgccaaa gcgcagtttg ccgcccactt
     8881 cgtcctggaa tttgttccac gcctgcgggg agttccagcc cggcgcccag gcaaacggca
     8941 cttgcgaacg gtgcgcggtc ggctggttgt taccttccat cgagaaggtg aacatggtgt
     9001 caatatcctg cggctgacgc ggctcatgaa cgctgatatt ggcgcgcatg gcggtacgac
     9061 cgctgtaacg gtgcggttca cgggccagtt tctgcccacg aatacggaat gtcgcatccg
     9121 gcgcagcatc tttgataccc gccagttctg ggattttcgc cacaacagcg tcaattacat
     9181 ggtcgagctg cgtccagtcc acttcacggc tcagcaaggt gctgtgcagc gagtgcagcc
     9241 agcgccagct ttccagcatg acagttttgc tgtcgtaata agccggatcg taaacctgga
     9301 agaaacgttg ggcgcgacct tcattattaa ttaccgtacc gtcgctttca gcaaagctgg
     9361 cagaggaaag caccagatgg gcgttttcca tgatcgctgt gcgttgatga tcaaccacca
     9421 tcaccagcgg cgctttagcc agcgcagcat tcacgcgggt agcagaagcg taacgatgca
     9481 gatcgttttc caacaccacc accgcgtcgg cgcgtccggt ttccagttcg gttaacgctt
     9541 cttcaagcga accgccaccc ataatgccca gccccatgct gttgacggaa cgggcaatca
     9601 tggtgatacc aacgtcagcg ccgcgacctt tcagggcttt cgcgacgtta gccgcagcct
     9661 gaatcacctc tatgctaccg gcgttcgtcc cggagataat caacggtttc ttcgcacctg
     9721 ccagtgcctg cacgatgacg tcgattttgt tttgcagctc aggttcgata ccgtcaaccg
     9781 ctggtgcgga gttatccagc gcatgggcaa tggcaaaacc taaacgcgcc tgatcttcaa
     9841 ccggtgcgcg gtaagtccac gccgcgatat catccagacg agtgtcatca acgttggtaa
     9901 caaacagcgg atgcttcgca cgttgaccga tgttgaggat tgccgcaatc tgccagtcag
     9961 ccactttctg tgctgcagcc atttcgcgcg ctttaccttt caccgcctga cgcactgcca
    10021 gtgcgacgcg cgcgccggtc tgggtaacgt cttcgcccag caccagtacc gcatcgtaag
    10081 attcgatttc gcgcagagcc ggagtataaa tgccgccttc acgcagcact ttcagcgcca
    10141 gttgcagacg ttcctgctca ccgtgagcga taccggtgta gaagttttct tcgcccacca
    10201 gttcacgcag cgcaaagttg ctttccacgc tggcacgcgg agaaccaata ccgatcactt
    10261 tcttcgactg acgcagaata tctgccgcgc cctgcattgc ctgttcggcg ttgagggtaa
    10321 tgaaatcatc gccacgacgc tgtactggct gacgcggacg atccttcagg ttgacgtaac
    10381 cgtaaccgaa acgaccacgg tcgcagagga agtagtggtt taccgtaccg ttgtaacggt
    10441 tttcgatacg acgcagttcg ccgtaacgtt caccggggct gatgttacag ccgatggaac
    10501 attgctggca gatgctcggc gcaaactgca tatcccattt acggttgtaa cgctcggagt
    10561 gcgttttgtc ggtaaatacg ccggtcgggc aaatttcgac caggttaccg gaaaattcgc
    10621 tttccagcgt gccgtcttcc gggcgaccga agtagacgtt gtcgtgcgcg ccataaacgc
    10681 ccagatctgt accgtcagcg taatctttgt agtaacgcac acagcggtag caggcgatgc
    10741 agcggttcat ttcgtgagag atgaatggcc ccaaatcctg attacggtgg gtacgtttgg
    10801 tgaaacggta gcgacggaag ctgtgtccgg tcatcacggt catatcctga agatggcagt
    10861 taccgccttc ttcacatacc ggacagtcgt gcgggtggtt ggtcattaac cactcgacca
    10921 cactttcacg gaactgtttc gcttcttcgt cgtcaatgga aataaaggtg ccatcggaag
    10981 ccggtgtcat acaggacatc accaggcgac cacgcgtgtc ttccgcgttt tggtattgct
    11041 tcaccgcaca ctggcggcaa gcaccgacgc ttcccagcgc cggatgccag caaaagtaag
    11101 gaatatcaag gcccagagac agacaagctt ccagcaggtt gtccgctccg ttgacctcgt
    11161 attctttgcc gtctacatga attgtagcca ttagcatgct tccagttttc tcggtcaggg
    11221 accgagcgtt aatcgaaatt cggttaccag cgctctttca gcaggttcgg ctgaatccca
    11281 ttaatcaaat gggtattgct gaacggctgt ttgattcccg cctcaaattc ttcgcggaaa
    11341 tatttgatgg cgctctgtaa cggctccact gcaccaggtg cgtgggcaca gaaagtttta
    11401 cccgggccta agaatcgaca cagttgctca agtgtttcga tatcgcccgg ctggccttcg
    11461 ccacgctcca gcgcacgcag aattttcacg ctccacggca gaccgtcgcg gcacggcgta
    11521 caccagccgc aggactcacg ggcgaaaaac tcttccaggt tacgcaccag cgacaccatg
    11581 ttgatctcat ggtcaaccgc catcgccagc gccgtaccca gacggctgcc cgctttaccg
    11641 atactttcga attccatcgg cagatcaagg tgcgcttcgg tcaggaagtc agtccccgcg
    11701 ccgcctggct gccaggcttt aaatttcaga ccatcacgca taccaccggc gtaatcttcg
    11761 aggatctcgc gtgcggtggt gccgaacggc agttcccaca gtcccggatt tttcacccga
    11821 ccggagaagc ccatcagctt ggtgccagca tctttacttt tcgagatgtt ctgataccac
    11881 tccacgccgt tagcgaggat cgccggaacg ttacacaggg tttcgacgtt gttgacacag
    11941 gtcggtttgc cccatacgcc ggaggttgcc gggaaggggg gtttcgagcg tgggttagca
    12001 cgacgccctt ccagggagtt gattaacgct gtttcttccc cgcagatgta gcgccctgcc
    12061 ccggtatgga cgaacagttc gaagtcgaaa cctgttccca taatgttttt gccaagcaag
    12121 cccgcttcag tggcttcggc aatggcgcgg cgcagattta ctgccgcttc gatatattcg
    12181 ccacgcagga agatgtagcc acggtaagct ttcagcgcaa acgcggagat gagcatacct
    12241 tccaccagca ggtgcggcag ttgctccatc aacaggcggt ctttataggt gcccggctcc
    12301 atttcatcgg cgttacacag caggtaacgg atgttcatgg attcgtcttt cggcatcagg
    12361 ctccacttca ggccagtcga aaagcccgcg ccgccgcgcc ctttcagacc agcgtctttt
    12421 acctgattaa cgatttcgtc cggagacagc ccggtcagcg ccttacgcgc cccttcgtaa
    12481 ccgtttttgc tgcggtattc gtccagccac actggctgtt tgtcatcgcg cagacgccag
    12541 gtcagcggat gcgtttcggg agtacggata atgtttttca tttataccgc tccagcagtt
    12601 cagggatcgc ttccggggtc agatgcgcgt gagtgtcctc atcgatcatc atgtttggcc
    12661 ctttatcaca gttccccagg cagcaagttg gcagcagcgt aaagcggcca tcaaatgtcg
    12721 tttgccctgg tttgatgttc agctttttct cgagcgccgc ctgaataccc tgatacccgt
    12781 tgatatgaca gaccacgctg tcgcaataac ggatcacatg gcgaccaacc ggctggcgga
    12841 agatctgact gtagaacgtt gccacacctt cgacgtcgct tgccggaata cccagcacat
    12901 cggcgatcgc gtggatcgca ccatccggca cccagccacg ctgcttctga acgattttca
    12961 gcgcttcaat ggacgccgca cgcgggtctt cgtagtggtg catctcgtgc tcaatcgctt
    13021 cacgctctgc cgcactcagc tcaaaagcct cggtttgtgg ttgttgattc tcgtgcataa
    13081 ttagcggtcc acatctgaca taacaaaatc gatactgccc agataaacaa tcaggtcaga
    13141 caccaggctg ccgcggatcg ccgccggaat ttgctgcaaa tgcgcataac tcggcgtgcg
    13201 gatacgagtg cgataactca tggtgctacc gtcgctggtc aggtagtaac tgttgatccc
    13261 cttggtcgcc tcaatcatct ggaaagattc attggcaggc atcaccggcc cccacgacac
    13321 ttgcaggaag tgggtgatca gggtttcgat atgttgcagc gtgcgctctt tcggcggcgg
    13381 cgtggtcagc gggtgatccg ctttgaacgg gccttccggc atgttgttga ggcactgctc
    13441 aagaatgcgc agactctggc gcagctcttc cactttcagc attacgcggg tgtagcagtc
    13501 agaaacacca ccaccaaccg ggatttcaaa gtcgaagttt tcatagccag aataaggacg
    13561 cgccttacgc acgtcgaagt cgatcccggt agcacgcagg cccgcgccag tggtgcccca
    13621 ctccagcgcc tctttcgcgc cataggcggc aacgccctgg gaacgacctt tcagaatggt
    13681 attttgcagc gccgctttct cgtaagacgc cagacgtttc ggcatccagt cgaggaactc
    13741 acgcagcaga cgatcccagc cgcgcggcag gtcgtgcgct acgccgccaa tacggaacca
    13801 cgccgggtgc atacggaaac ccgtgatcgc ttccaccaga tcgtaaattt tctgacgatc
    13861 ggtaaaggcg aagaacacgg gcgtcattgc gccgacgtcc tgaataaagg tcgagatgta
    13921 cagcaggtgg ctgttgatac ggaacagttc ggagagcata acgcgaatga cgttaacgcg
    13981 atccggcacg gtgatcccgg ccagtttctc taccgccagc acgtaaggca tttcgttaac
    14041 gcagccgccg aggtattcaa tacggtcggt atacggaatg tagctgtgcc aggactggcg
    14101 ctcgcccatt ttctccgcac cacggtggtg gtagccgatg tctggtacgc agtcgacaat
    14161 ctcttcgccg tcgagttgta aaacgatacg gaaagccccg tgcgccgacg ggtggttcgg
    14221 accgaggttg aggaacatga agtcctcatt ttcggtgccg cgtttcatcc cccactcttc
    14281 cggtttgaag gtcagggctt ccatctccag atcctgtttg gctttggtca gctcaaacgg
    14341 cgagaattcg gtagcgcgtg ccgggtaatc tttacgcagc gggtgacctt tccaggtttg
    14401 cggcatcatg atgcggcgca ggttcgggtg accgtcgaaa gtaatgccaa acagatccca
    14461 ggtttcacgc tcataccagt tagcgttcgg gaacagtttg gtgaaggtcg gtacgtgcag
    14521 gtcgttttct gccagcgcca ccttcagcat gatgtcgcgg ttacgatcga tagaaatcag
    14581 atggtagaaa acggaaaaat ccgcggcagg taacccttcg cggtgtgtgc gcagacgttc
    14641 gtccatgccg tgtaagtcaa acagcatgac gtaaggtttc ggcagtttct ttaagaaatc
    14701 gccaacttcc agtaattgtt cacgcttgat ccacacaacg ggaaccccgg tgcgagtcgc
    14761 ctgaacagta aaggcatccg gcccaaaacg gttgcgcagt tcgccaatta ccggatcatc
    14821 aagatgatcg cgggtctgcc aggcgggttc ttgcgcggtt aagtcagtca tattgttcac
    14881 cattgcaaat ggtccgtggt gactgtcggc gcagtaagct tcgcgaaata gatttaggaa
    14941 taagcgaaga aaaatccctt tgccgacagg cgcaaattaa atctcgtcag gtgtacgcag
    15001 gttggttacg gcaatgcgtt caccgcgctt gcgttcgcgc tctgattgca tattggcgcg
    15061 ataaacgccc tgatcgccaa ccacccagga gagcggacga cgttctttgc cgatagattc
    15121 ctgcaacagc atcagtgcct gcatgtacgc ttcaggacgc ggcgggcagc ccgggatata
    15181 cacatcaacc gggatgaatt tatcgacgcc ctgcacaacg gaataaatat cgtacatacc
    15241 accagagttg gcacaggcac ccattgagat aacccatttt ggttccagca tctggtcata
    15301 cagacgctga ataaccggtg ccattttggt aaagcaggtt cctgcaacca ccatcaggtc
    15361 agcctgacgc ggcgaagcac gcaatacttc tgcgccaaaa cgcgccacgt catgcaccgc
    15421 ggtaaacgaa gtcaccatct caacgtaaca gcaggaaaga ccgaagttat acggccaaat
    15481 tgagttttta cgaccccagt taaccatgtc attgagcttg cccataaaca cgtttttgtt
    15541 aacttcttgc tccagagggt cggttacgat ctcctgcttt tgcagggggt aacggtcgtt
    15601 ctcaccgttg ggatctatgc gggtgagcgt ataatccatc ttaatgcctc gcggttagcg
    15661 ttgacgatta gcgatactgt tcgtttccgg gttcatacgc tcgcggcgtg aacgcgcggg
    15721 cgtccagtcc agcgcgccaa tacgcaccag ataaaccaga cctgccagta acacaaaaat
    15781 aaaaattgca gcttccacaa agcctaccca gccgctttcg cggatagagg ttgaccatgc
    15841 gaacagatac agcgcttcaa cgtcgaagat aacgaagaac atggccacca gataaaactt
    15901 ggcggacagg cgtaagcggg cggagccgac cgagtcgata ccggattcaa acggcacgtt
    15961 tttcgacctc gcgcgtgcgc gaccgcctaa aaaccaaccg cctaccagca tcaggcaaca
    16021 caggccaatg gcaacgataa gaaagatagc gaatgcccag tgatgagcga tgacttcagt
    16081 ggatgttgac atactcattg cttactcatc aaaagtagcg ccagattcac tgctcttcac
    16141 ggcagatgga cgccacatcg attcatgggg aggaataaaa aaaaccttac aataactgta
    16201 gaaattcttt tatacagcta attgatgtgg tcttttactc ctttctataa ccttttgtca
    16261 actttaacaa aagcttcttc acattagttt acataatatc aacaccatta gcatttaatg
    16321 ccctttcacc ccagatcctt gacgactcca ggataattag atgttgttga atcgtgtccg
    16381 ttgtgaagca atggaaaaaa tacgggtcta ttttgacagg aatttgtgtc gattcctccc
    16441 cccaaaagag agtattttct tgatctgtga cacgcttttg ttattccata acaaaaacgc
    16501 agcaacaaat ttacgtattt tttaacatca ttgtagcagg tgattttttt caggcgatta
    16561 tttgtgcatt cgggacgtga atctctggtg attgaaaaat aaacagtttt gtacgttctg
    16621 cactatgcga ggaaggattt ttactaaaaa aaagccgctg gggtttaaaa cacccccagc
    16681 ggctcgtttt ttacactatt gtctcaggaa ttatctatcg tccgtcgatt actcgatatc
    16741 cctttcaatc aacaatgaat catccccttc cggagcagac attgggctgt attgccacgg
    16801 attatggtag ctctccatcg cctgatagat cacctgggcc agctcattat tactggacgg
    16861 gtcatagcac agcaaatatt cggtatcagg tagcggcggt aagccatcta ctccactcag
    16921 cacacgcagg tccgggctca tcatctcaac cggccttgcc gtcacgccaa gaccggcttt
    16981 cactgccgca cgaacggccg gaagcgtcga ggcgacataa gccagtcgcc atggaatatc
    17041 tactttatta agcgtcgcca gcaccatatc gcgaaacgga cttggatcat ccagcaatac
    17101 aagagggatc ggctcgcctt tttgcaatac gtattccgct gcgcagtacc agtgtgttgg
    17161 cgaggtacgc aggttcaatg ccttaaacgc tgaaggacga tgggtggtta ccatcaaatc
    17221 cacttcctgg gattccagca tttctgccat ataggcatta cgtttaacgc ggacatccag
    17281 cgctaatttc ggataaaccg aactcacgcg atttaacagg aaaggtaaga tcgtatcggc
    17341 agattcatct gaagcaccga tagttaacac gccctgaaga ttactgaaca ttaatgatga
    17401 gcaggcctca tcattaaaac gcaggatttt cctggcgtaa ccaagaagtt gaatgccatg
    17461 ttcagttaac agtttgttgc gaccgtgacg agcgaacagt tctttcccaa cgagttgttc
    17521 cagacgctgc atttgctgac ttacggcgga ctgagtacga cacacagcgg cagctgcggc
    17581 agcaaaagtg ttcagatcgg caacagcaac aaatgttctc agcagatcga ggtcgaggtt
    17641 aattatcgga cgatttgcac ttatcatata ttatcactta ctggcggctc atactgagct
    17701 ggttaatgct gtgcacacac aaacaagcaa ttccatttgt aatgtgcctc cctggcagtt
    17761 tcatccggaa cccggacgaa agtaaaaatg catatgagtt gcactaaaaa agcgactcac
    17821 attgttccgt tataatgcct gaagtagatc acagaatata tcttcaggga tcgcatatct
    17881 attaagttac tcactctttt ctatttatga catgcgcgtg tttgtataaa tgtaaatgtg
    17941 agtccttgtt ccactctcgt gcagcatcgc tggtcatacg cgaacacgta caaacagcaa
    18001 tggtgaagcg gcatcaagag cagggatccg tcatttatgc gagcatttta ccccaaaaac
    18061 cttttattta taagggtcat tgcgaattat ctgatgcaaa gttatgttat gttaggcaaa
    18121 gtaatcttct attattaata agcacatcaa aacctttttg aatattaaat aataattaat
    18181 tagcatcatc ctcattcatt aattccgctt aacaatagtt tcacacctta cccccattgc
    18241 gacacctcgg aaatcatcac gctgttaaac tctccgtaat gcggaaatat catcatcgcg
    18301 tttcattagt gaattcttaa atgaggcatt ttcacacaat tatcttacag ataaaaaaac
    18361 cagacttgca attaagaatt agaacaattc actatataac attgcatgta aagcatataa
    18421 gcctaattat ttgtcattat taagttatta acagcacaat cgagccttcc cctctggcaa
    18481 aatcttattc tgcagacctt caaaacaccg tcctggggga gtacattgtt ctaagctgac
    18541 ttccacggca gggagtggcg ataacagcaa aaaaggtcaa gattcatgtc ccccattgaa
    18601 aaatccagca aattagagaa tgtctgttat gacatccgtg gtccggtgct gaaagaagca
    18661 aaacgcctgg aagaagaagg taacaaggta ctgaaactga acatcggcaa cccagcccca
    18721 ttcggttttg acgcgccaga tgaaatcctc gttgacgtga tacgcaacct gcctaccgct
    18781 caagggtatt gcgattccaa agggctttac tccgcgcgta aagccatcat gcagcactac
    18841 caggctcgtg gcatgcgtga tgttaccgtg gaagatattt acatcggcaa tggtgtatcg
    18901 gagcttatcg ttcaggcaat gcaggcattg ctgaacagcg gggacgaaat gttggttcct
    18961 gcaccagatt acccactctg gaccgcggcg gtttcgcttt ccagcggtaa agcggtgcat
    19021 tatctttgcg atgaatcctc tgactggttc ccggacctcg atgatattcg cgctaaaatt
    19081 acgcctcgta cgcgtgggat tgttattatc aacccaaata acccaaccgg cgcggtatat
    19141 tccaaagagc ttttaatgga gattgtggag attgcacgtc agcataatct cattatcttc
    19201 gccgatgaaa tttatgacaa aattctctac gacgatgctg agcatcactc aattgcgccg
    19261 ctggcacctg acctgctgac cattaccttt aacggactgt cgaaaacgta ccgcgttgca
    19321 ggcttccgtc aggggtggat ggtgttgaac gggccgaaaa aacacgccaa aggctacatc
    19381 gaaggtctgg aaatgctggc ttcaatgcgc ctgtgtgcta acgttcctgc gcaacacgcc
    19441 attcagaccg cgctgggtgg ttatcagagc atcagtgaat ttattacccc tggcggtcgt
    19501 ctttatgagc agcgtaaccg cgcgtgggaa ctgatcaacg atattccggg cgtttcctgc
    19561 gtgaaacctc gtggtgcgct gtatatgttc ccgaaaatcg acgccaaacg ctttaacatt
    19621 catgacgatc agaaaatggt gctggatttc ctgttgcagg aaaaagttct gttggtgcaa
    19681 gggacggcat tcaactggcc gtggccggat cacttccgca ttgtcacgct accgcgtgtc
    19741 gatgatatcg agctgtcttt gagcaagttc gcgcgtttcc tttctggtta tcatcagctg
    19801 taatcttaat ttcactgccg gagattgcat ccggcagtgt tatcccgcca caatgacctg
    19861 atgatgtcat catacgtaag gtcactatga aacagagcca tttttttgcc catctctccc
    19921 gcctgaaact cattaaccgc tggccgctaa tgcgcaacgt gcggacggaa aatgtgtccg
    19981 aacacagttt gcaggtagcg atggtcgccc atgcgctggc agctatcaaa aatcggaaat
    20041 ttggcggtaa tgtcaacgcc gaacgtatcg ctttactggc gatgtaccac gatgcctcag
    20101 aagtgctcac cggcgatctc cctactccgg tgaaatactt caatgcgcaa atcgctcagg
    20161 agtacaaggc tattgaaaaa atcgctcagc agaaactggt cgatatggtt ccggaagaac
    20221 tgcgggatat ctttgcgccg ttaattgacg agcatgcata tagcgatgaa gaaaaatcgc
    20281 tggtgaaaca ggcagatgca ctgtgtgcat atctgaaatg tctggaagaa ctcgcggccg
    20341 gaaataatga attcttgctg gcaaaaacgc gactggaagc gacgcttgaa gcgcgtcgca
    20401 gccaggagat ggactacttc atggaagtat ttgttcccag cttccatctt tcgctcgatg
    20461 agattagcca ggattcaccg ctgtaagcag ccggagtctg cgtcgcatca gtcaataagc
    20521 gccggatgcg acatcaggct cttgtcaaaa cggaaacagc atcgggatca tcaccacaca
    20581 aaccgccatc acgataatgg tgaacggtac ccccaacttc acaaagtcac tgaagctgta
    20641 atttcccgga cctaaaacca gggtgttaac aggtgaagac accggtgtca taaaggcggc
    20701 ggatgctgcc atcgccacga ccatcgcaaa tggataaggc gacactccca tcgttttggc
    20761 agcagccagc gcaatcggag ccatcaacac cgccgtcgcg gtattagaga taaacagccc
    20821 aataaccgcc gacaagacaa acaaacaccc cagcatcata tgtggcccat aaccgccgcc
    20881 aatatccatc agccctttca ccgccagcgc gacacctccc gttttctgta atgccacagc
    20941 aaacggcatc atcccaacga tcaaaataat gctcggccag tgaatggatt tataggcgct
    21001 ttcagcatct atacagcgga atttccccat cagcaggcag gcgatgatag cggcaacagg
    21061 attaggaatt tcatttgtca gcattaacgc caccattagc accagacaga aaatggcatg
    21121 gggtgcctgg ctgtgcgcgg gcgatgcttc actcacctct tccggtaagt tcagcgctac
    21181 gaagtcgcgg ccctgtttgg ccagcatacc gatcagtttc cagttaccca caaccaggat
    21241 gatatcgccc agcagcagag gctcatccgc cagcgaacct tccagcgcca cgccattgcg
    21301 cttgagcccc accacattca gtccgtagcg ggtacgaaaa ccaatttcgc gcaccgattt
    21361 accaatcagt tctgactcag gaattaatga aatctctgcc atgcccacat caagggcctg
    21421 gtcagaaaaa tactcgccgc gcagtaccat cggctccagc aattgctcac tacaaaattg
    21481 ccggagatcg acatcagccg cagacatatc aataagcaaa acgtcacgcg cgcgaaattc
    21541 agaaacccca ttaacgttca cgataacgcg acgaaaacgc cgccagcgtt caacaccgat
    21601 gacgttagcg ccataacgct cacgtaattt gagatcatcc agccgttgac ctatcattgg
    21661 cgatccgggg cgaatagcca gacgtcgcgc acgcccggtc agtcgatatt cacggataag
    21721 atcgcgaaag gttcgacgcg tccagccttc gcgctgcggg gtctgggtat cccctttcag
    21781 catgaaacgc atcactaaca tatacaaaat acccagcacc agcacgacca ggccaatagg
    21841 tgttacgcta aagaaactga agccgtgata gccttcacgc agcaattcac tgttgactac
    21901 caggttcggc ggcgtcgcca ccagcgtcat catgccgcta atcagcccgg caaaacttaa
    21961 cggcatcatc agacgtgacg gcgaagtttg catacgcatg gcaacgctta acaccacggg
    22021 aataaagata gcgacaacgc tggttgaact cataaacgcg ccaagccccg cgacggtcag
    22081 catcaacaaa accaacattt tgatttcact gttgcccgcc actttgacca gccatgttcc
    22141 cattacggtg gcaacaccgg tacggaccaa accatcgcca ataataaaca aggcggcaat
    22201 caggacaacg ttaggatcag aaaagccgga aaatacttcg gggaccgtca gcgttccgct
    22261 taatgcaaac gcgacaataa caaacaaagc gaccgcatcc atacgcactc tgcccgtcgc
    22321 aaacaagacg atggcaacgg ccagtaatga aagaacccaa atcaattcac cgttcacaac
    22381 ttatccttgt taattgaggg ggatgacttg attctgccat aaaaaagccc cgacgagacg
    22441 gggctaaatc atgatcaggt gtttcactga ataataacat cgccatttgg ctgtttggtc
    22501 acagtaattt gctccagact gtggaggacc aaatcgacct cattcaggcg cggggtatct
    22561 gccggagcgt taaccgcaat gacatggcaa cccgccgcca ggccagaaag cacgccagcg
    22621 ggagcatctt ccaccaccac acactcctgc ggcgcaagcc ccagcagctg cgcacctaac
    22681 agatacgcat caggttctgg ttttccgcgc ttcactcgct cagcggttac aaacacctct
    22741 ggtgcgggaa gccccgctat tttatggcgc gctcgcgcta ccggcatgga gccagaagtc
    22801 acaatggccc acggaatacc tgctttattc aaatgattga gtaaggcgat tgccccagga
    22861 agcgcggtaa taccttcggt ttccgtggcc tcgatttgct ccagacgcgt aaactcggcg
    22921 gcaatatcag cctcggattt gcccgccata aaatggcgca aagaggtgat cgcctgttta
    22981 ccgtgaatga aagccagcac ctcttccggc gctaacccat gacgtctggc ccagttgctc
    23041 cacgcccgtt ctaccgcagg cagggaatcc accagcgttc catcaagatc aaacagaaaa
    23101 cctttgcacc gcacgcgggc ctcctcaggc gttgataatt tgattaattt cgttggcgct
    23161 caaatggtac tgacgcgggc aggcatgcca cacattaagc atgcgctgat atttttccca
    23221 cattggcgtc tgggcgttaa aaccatgagt cccagcgtca aaatgggtat agcgcccttc
    23281 cacattaacc ataaagcgga cataaccgag gtaacgtgct tcagtggcgg cgtcaaagcc
    23341 gaggaaggtg acacgacgtt catcgatgga ttgctggtcc tgcaaattag accaagaaac
    23401 atacaacgca tgatacatat ccataatgtc gatgatagtg cggcaggttt cttctttcag
    23461 ctcgccaaac tcgcgatcca attcacgcat ctgtaatccg taaccacgct caataattgt
    23521 ttgcaggcga cggtaacgtt cagcatttgc cggatcgagc atagtcatca tcttgtactg
    23581 attagacaaa ataagacgtt gcgcgttggt catttccatt gttgactcct gtatcactct
    23641 actacggtga aaaaaaagaa ggctgagtat gccttctttt atatgcgtaa tcaggggtca
    23701 attacaaatc atcaaggaaa gttttatcca gttgtttgaa ggcgcgctta agcgtgtcag
    23761 ctaatgcctg gtaatcaggc ttgccttcaa cgggtgccaa cacctgtcca gactcctgca
    23821 atttaccgcg aacttcataa aaccagttaa ggattgcagg gggtaatggc gttacagaac
    23881 gcttgcccag ccaccacaat ccctgcatgg gtaaacttaa ggcgaacagt gcagtggcaa
    23941 ctgccggccc aagcagaccg cccagggcaa tctgccagca gagagtaaat acggcgatcg
    24001 gcggcataaa acggatcgca taacgcgtca tcttgataac gcgattttcg acaaagaccg
    24061 gagcaaggcg tttttccagt ggccacgtct ttgagtaatg ctgtccgcgg cgaaacaagc
    24121 taaaaaaatt aacagaacga ttatccggcg ttgacatgct tcacctcaac ttcacatata
    24181 aagattcaaa aatttgtgca aattcacaac tcagcgggac aacgttcaaa acattttgtc
    24241 ttccataccc actatcaggt atcctttagc agcctgaagg cctaagtagt acatattcat
    24301 tgagtcgtca aattcatata cattatgcca ttggctgaaa attacgcaaa atggcataga
    24361 ctcaagatat ttcttccatc atgcaaaaaa aatttgcagt gcatgatgtt aatcataaat
    24421 gtcggtgtca tcatgcgcta cgctctatgg ctccctgacg tttttttagc cacgtatcaa
    24481 ttataggtac ttccatgtcg agtaagttag tactggttct gaactgcggt agttcttcac
    24541 tgaaatttgc catcatcgat gcagtaaatg gtgaagagta cctttctggt ttagccgaat
    24601 gtttccacct gcccgaagca cgtatcaaat ggaaaatgga cggcaataaa caggaagcgg
    24661 ctttaggtgc aggcgccgct cacagcgaag cgctcaactt tatcgttaat actattctgg
    24721 cacaaaaacc agaactgtct gcgcagctga ctgctatcgg tcaccgtatc gtacacggcg
    24781 gcgaaaagta taccagctcc gtagtgatcg atgagtctgt tattcagggt atcaaagatg
    24841 cagcttcttt tgcaccgctg cacaacccgg ctcacctgat cggtatcgaa gaagctctga
    24901 aatctttccc acagctgaaa gacaaaaacg ttgctgtatt tgacaccgcg ttccaccaga
    24961 ctatgccgga agagtcttac ctctacgccc tgccgtacaa cctgtacaaa gagcacggca
    25021 tccgtcgtta cggcgcgcac ggcaccagcc acttctatgt aacccaggaa gcggcaaaaa
    25081 tgctgaacaa accggtagaa gaactgaaca tcatcacctg ccacctgggc aacggtggtt
    25141 ccgtttctgc tatccgcaac ggtaaatgcg ttgacacctc tatgggtctg accccgctgg
    25201 aaggtctggt catgggtacc cgttctggtg atatcgatcc ggcgatcatc ttccacctgc
    25261 acgacaccct gggcatgagc gttgacgcaa tcaacaaact gctgaccaaa gagtctggcc
    25321 tgctgggtct gaccgaagtg accagcgact gccgctatgt tgaagacaac tacgcgacga
    25381 aagaagacgc gaagcgcgca atggacgttt actgccaccg cctggcgaaa tacatcggtg
    25441 cctacactgc gctgatggat ggtcgtctgg acgctgttgc attcactggt ggtatcggtg
    25501 aaaatgccgc gatggttcgt gaactgtctc tgggcaaact gggcgtgctg ggctttgaag
    25561 ttgatcatga acgcaacctg gctgcacgtt tcggcaaatc tggtttcatc aacaaagaag
    25621 gtacccgtcc tgcggtggtt atcccaacca acgaagaact ggttatcgcg caagacgcga
    25681 gccgcctgac tgcctgattt cacaccgcca gctcagctgg cggtgctgtt ttgtaacccg
    25741 ccaaatcggc ggtaacgaaa gaggataaac cgtgtcccgt attattatgc tgatccctac
    25801 cggaaccagc gtcggtctga ccagcgtcag ccttggcgtg atccgtgcaa tggaacgcaa
    25861 aggcgttcgt ctgagcgttt tcaaacctat cgctcagccg cgtaccggtg gcgatgcgcc
    25921 cgatcagact acgactatcg tgcgtgcgaa ctcttccacc acgacggccg ctgaaccgct
    25981 gaaaatgagc tacgttgaag gtctgctttc cagcaatcag aaagatgtgc tgatggaaga
    26041 gatcgtcgca aactaccacg ctaacaccaa agacgctgaa gtcgttctgg ttgaaggtct
    26101 ggtcccgaca cgtaagcacc agtttgccca gtctctgaac tacgaaatcg ctaaaacgct
    26161 gaatgcggaa atcgtcttcg ttatgtctca gggcactgac accccggaac agctgaaaga
    26221 gcgtatcgaa ctgacccgca acagcttcgg tggtgccaaa aacaccaaca tcaccggcgt
    26281 tatcgttaac aaactgaacg caccggttga tgaacagggt cgtactcgcc cggatctgtc
    26341 cgagattttc gacgactctt ccaaagctaa agtaaacaat gttgatccgg cgaagctgca
    26401 agaatctagc ccgctgccgg ttctcggcgc tgtgccgtgg agctttgacc tgatcgcgac
    26461 tcgtgcgatc gatatggctc gccacctgaa tgcgaccatc atcaacgaag gcgacatcaa
    26521 tactcgccgc gttaaatccg tcactttctg cgcacgcagc attccgcaca tgctggagca
    26581 cttccgtgcc ggttctctgc tggtgacttc cgcagaccgt cctgacgtgc tggtggccgc
    26641 ttgcctggca gccatgaacg gcgtagaaat cggtgccctg ctgctgactg gcggctacga
    26701 aatggacgcg cgcatttcta aactgtgcga acgtgctttc gctaccggcc tgccggtatt
    26761 tatggtgaac accaacacct ggcagacctc tctgagcctg cagagcttca acctggaagt
    26821 tccggttgac gatcacgaac gtatcgagaa agttcaggaa tacgttgcta actacatcaa
    26881 cgctgactgg atcgaatctc tgactgccac ttctgagcgc agccgtcgtc tgtctccgcc
    26941 agcgttccgt tatcagctga ctgaactggc gcgcaaagcg ggcaaacgta tcgttctgcc
    27001 ggaaggtgac gaaccgcgta ccgttaaagc agccgctatc tgtgctgaac gtggtatcgc
    27061 aacttgcgta ctgctgggta atccggcaga gatcaaccgt gttgcagcgt ctcagggtgt
    27121 agaactaggt gcaggcattg aaatcgttga tccagaagtg gttcgcgaaa gctatgttgg
    27181 tcgtctggtc gagctgcgta agaacaaagg catgaccgaa accgttgccc gcgaacagct
    27241 ggaagacaac gtggtgctcg gtacgctgat gctggaacag gatgaagttg atggtctggt
    27301 ttccggtgct gttcacacta ccgcaaacac catccgtccg ccgctgcagc tgatcaaaac
    27361 tgcaccgggc agctccctgg tatcttccgt gttcttcatg ctgctgccgg aacaggttta
    27421 cgtttacggt gactgtgcga tcaacccgga tccgaccgct gaacagctgg cagaaatcgc
    27481 gattcagtcc gctgattccg ctgcggcctt cggtatcgaa ccgcgcgttg ctatgctctc
    27541 ctactccacc ggtacttctg gtgcaggtag cgacgtagaa aaagttcgcg aagcaactcg
    27601 tctggcgcag gaaaaacgtc ctgacctgat gatcgacggt ccgctgcagt acgacgctgc
    27661 ggtaatggct gacgttgcga aatccaaagc gccgaactct ccggttgcag gtcgcgctac
    27721 cgtgttcatc ttcccggatc tgaacaccgg taacaccacc tacaaagcgg tacagcgttc
    27781 tgccgacctg atctccatcg ggccgatgct gcagggtatg cgcaaaccgg ttaacgacct
    27841 gtcccgtggc gcactggttg acgatatcgt ctacaccatc gcgctgactg cgattcagtc
    27901 tgcacagcag cagtaatctc gtcatcatcc acagctttgc gctgcggata tctgaaccgg
    27961 aaataatcac tatttccggt tttttattct cttaatttgc attaatcctt tctgattatc
    28021 ttgcttaact gcgctgcatc aatgaattgc gccatctcac tttgcatact taccactttg
    28081 ttttgtgcaa gggaatattt gcgctatgtc cgcaatcact gaatccaaac caacaagaag
    28141 atgggcaatg cccgatacgt tggtgattat cttttttgtt gctattttaa ccagtcttgc
    28201 cacctgggta gttccggtgg ggatgtttga cagtcaggaa gtgcagtatc aggttgatgg
    28261 tcaaacaaaa acacgcaaag tcgtagatcc acactcattt cgcattctga ctaacgaagc
    28321 aggcgaacct gagtatcacc gcgtacagct gttcacgacg ggcgatgaac gcccgggcct
    28381 gatgaacttc ccgtttgaag ggttaacctc aggatcgaaa tacgggacag ccgttggcat
    28441 catcatgttt atgctggtga ttggcggcgc gtttggcatt gtgatgcgta caggaaccat
    28501 tgataacggt atcctggcgc ttattcgcca tacccgcggg aatgaaattc tctttattcc
    28561 tgcgctgttt attctgtttt cacttggcgg cgcggtattt ggtatgggag aagaggccgt
    28621 cgcctttgcc attatcatcg caccgctaat ggtccggctg ggctatgaca gtattaccac
    28681 cgtcctggtg acttatattg ccacgcaaat cggttttgcc agttcgtgga tgaacccgtt
    28741 ttgtgtggtt gttgctcagg ggattgccgg cgttccggtg ctttctggct ccgggttgcg
    28801 catcgtggtg tgggttatcg ccactctgat tggcctgatc tttaccatgg tgtacgcctc
    28861 acgagtgaaa aagaatcctc ttctgtcacg cgtgcatgag tccgaccgct tctttcgtga
    28921 aaagcaggcg gatgttgaac aacgtccgtt tacctttggt gactggctgg tattgattgt
    28981 cctgacagcc gtaatggtct gggtgatttg gggcgtgatc gttaatgcct ggtttattcc
    29041 agaaattgcc agccagttct tcaccatggg tctggtgatt ggcatcatcg gcgttgtttt
    29101 ccgccttaac ggcatgacgg ttaataccat ggcttcatcc tttaccgaag gggcgcgaat
    29161 gatgatcgcc cctgccctgc tggtgggttt cgccaaaggg attttgctgc tggttggtaa
    29221 tggtgaagca ggtaatgcca gcgtgttaaa tactatcctc aacagcattg ccaatgccat
    29281 tagcggtctg gacaacgcgg tcgcggcctg gtttatgttg ctcttccagg ctgtatttaa
    29341 tttcttcgtg acgtccggtt ctggtcaggc ggcattaact atgccgttac tggcaccgct
    29401 tggcgatctg gtcggtgtta accgtcaggt taccgtgctg gcgttccagt ttggtgatgg
    29461 cttcagccac atcatttacc caacctcagc ttcgttaatg gcaacgctcg gtgtgtgccg
    29521 ggtggacttc cgtaactggc tgaaggtggg cgcgaccctg cttggactgc tgtttattat
    29581 gtccagcgtc gtagtgatcg gcgctcagtt gatgggctac cactaaaaat gttaagagcc
    29641 gcaattgcgg ctcttttttc attctgccgt ttcagtctct acggcttcat ttttggcatt
    29701 gcgcttcatc cacaacgcca gcgctttcag cgagtctgga gtgaactcat cgcagcgtgc
    29761 ggtgatttct tccggcgtca gccagcaaac tccactgact tcatcttcct gtagggcgaa
    29821 gggaccgtga gagacgcaac tgaacaacgc gccccagaca cggcaatttt tatcttcgaa
    29881 atagaactgc ccgtgctcgg caaaggggac accggcaatg cccaactctt cttccgcttc
    29941 gcgacgcgcg gattccagca gttgctcatc ggcctgaact acaccgcctg cggtcgcatc
    30001 taacatgccg ggtaaaaagt cttttgtctc ggtacgacgc tggaccagaa ttttgcccat
    30061 gccatcatgc acgacgatgt aagttgcacg atgacgcaga cactgtgccc gcatttgttc
    30121 ccggctggct tgtgcaatga cttcgttctc ttcattgaca atatccaccc attcagtact
    30181 tgccaaacga cgctgttcca ttatcaggaa accttctttt tctggcgctc ttacggcgca
    30241 ttttgagttg tgggtaaatt acggattaat cgcgacctgc gcaatgatac tttgatcatt
    30301 gagtgcgatt acactaagta cgtcattatc cagcatgcca taacccgccg gattaccgcc
    30361 tttcggaata ctcaccgagc cggggttgaa gtggaaaatc tccccccgct gttctgccac
    30421 tggcagatgg gtatgaccgt acaccagcac atcgttctgg tttaaagcag gtagattttc
    30481 cgggccaaaa agatgaccat gcgtcaaaaa cagacgttgt ttttccagta atacctgttg
    30541 ccacggcgcg gttatcggga aatgcagcat catttgatcc acttcgctgt cgcagttgcc
    30601 acgcacagcg ataaccttat gtgccacttc attaagccgt tcagcgactt tggctggcgc
    30661 gtaaccctcc ggtaaagcat tacgcgggcc gtgattcaac acgtcgccaa ggatcaccag
    30721 ccactgggca ccgctttgga caaacaactc cacaacacgt tccgtcgccg gtaacgaccc
    30781 atgaatgtcc gatgcaaaca tcagtttcat cactcactcc tcgtcgaaaa aacgtaagct
    30841 atgatactgg attctgtcgt tgctatcagc cagattgctt cgccgaaagt gcaataaaac
    30901 gctggacaga cgctcgctgc cactggaatg tcgcataatc caccaggcgt tctggcacct
    30961 catcgccatg tagtaccagg cggttaatca ttagcgccag atcagtatca gcaatgcacc
    31021 attcaccaaa taaattcggc tgacccagtg ctaacaaatg ttctgccatc gcgaacagtt
    31081 tctctgcact ggcttttccc tcggccgtta gtggcgcttt tttcgccccc gcaaagacaa
    31141 catccgtcgg acgctcttcg cggatgggca tcagatcgct gcgcagccag gcctgaatct
    31201 gtcgtgcacg cgcacgattt tctaaatcaa gcggataaat acgttcccag gtcggtggcg
    31261 caaatcgatc ttccagatac tccgcaatgg cagaagattc actcaactca aaatcatcga
    31321 tttgtaataa cggcacacgg cgtgtctgac cgtaaccttg ccacgtcggc tgcaaatgtt
    31381 caccgctgtc gaggtcgatg gtcttgatat gaaacgacag gcctttttcc tgcaacgcca
    31441 cccaggcgga taacacataa ggggagaaaa agtgggcatc tgaccaaagc gtgatagcgg
    31501 gtttactcat tatgtcctcg gcaataaagg gggctggcta tcaaactata gagccttttt
    31561 gtcactgtca cgtgacgaat actcacgtaa gtcgccgcgc tttctatact tgtggtgttc
    31621 agatgattaa acaaccggag ctgcaatgat cgatctctat ttcgccccga cacccaatgg
    31681 tcacaaaatt acgctgtttc tcgaagaagc agagcttgat tatcgcttga ttaaggtaga
    31741 cttggggaaa ggcggtcagt ttcgtccgga atttttgcgc atttcgccca acaacaaaat
    31801 tccggcaatt gttgatcatt ctcctgccga tggcggcgaa ccgttaagcc tctttgaggc
    31861 tgctgccatt ttgttgtatc tggctgagaa aacaggactc tttttgagtc atgaaacgcg
    31921 tgagcgcgcc gccacattac agtggttatt ctggcaggta ggcggactgg ggccgatgct
    31981 tgggcaaaat catcatttta atcacgcagc cccccaaacc attccttacg ctattgaacg
    32041 ttatcaggtt gaaactcagc gtctttacca tgtactaaac aagcggctgg aaaactcgcc
    32101 ctggctggga ggcgagaact acagcattgc ggatattgcc tgctggccgt gggttaatgc
    32161 ctggactcgc ctgcgaattg acctcgcaat gtatccggca gtcaagaact ggcatgagcg
    32221 gatccgttcg cgccctgcca ccgggcaggc actgctaaaa gcacaactcg gtgatgagcg
    32281 ttcggatagt taacagaaac aggttctcgt gtattatttc atcctaagta aaacaacgga
    32341 gaacctgcaa tggcacaacc tgccgctatt attcgtataa agaaccttcg tttgcgtacg
    32401 tttatcggaa ttaaggaaga agaaattaac aaccgtcagg atattgttat caatgtgacg
    32461 atccactacc ccgccgataa agcgcgcact agcgaagata tcaacgatgc gctgaattat
    32521 cgcaccgtaa cgaaaaacat tattcagcat gtagagaata accgtttctc tttgctggaa
    32581 aaattaactc aggatgtgct cgatatcgca cgtgaacatc actgggtgac gtatgctgaa
    32641 gtggagatcg ataaactgca cgcgctgcgc tacgccgatt cggtatccat gaccttaagc
    32701 tggcagcgtt aatcgccata tcgggaggct gcatgaacat agtgatcacc ggagggacgg
    32761 gattaattgg tcgccatttg attccgcgtt tgctggagct gggccatcaa attacggtag
    32821 tgacgcgtaa cccgcagaaa gccagttccg ttctcggccc tcgggtgaca ctatggcaag
    32881 ggcttgccga tcaaagcaac ctcaacggcg ttgatgcggt aatcaacctg gccggagaac
    32941 cgattgctga taaacgctgg actcacaagc aaaaagagcg tctctgccaa agccgctgga
    33001 atatcacgca aaaactggtc gatttgatta atgctagcga cacgccgccg tcggtactca
    33061 tttccggctc ggcaacgggc tattatggcg acttaggtga agtggtggtt accgaagagg
    33121 aaccgccgca taacgaattt actcataaac tctgcgcccg ctgggaagag attgcctgcc
    33181 gggcgcaaag tgacaaaacg cgagtgtgcc tgctgcgaac cggcgtggta ctggcaccgg
    33241 acggcggtat tctcggtaaa atgctgccac cgtttcgtct tggcctgggc gggccgattg
    33301 gttccggtcg gcagtatctg gcctggattc atattgatga tatggtcaac ggcattctct
    33361 ggctgctgga taacgagctg cgcgggccat ttaatatggt ttcgccctac ccggtacgca
    33421 atgaacaatt tgcccatgcg ctcggtcatg cgctgcatcg cccggctatt ttgcgcgtcc
    33481 ccacaaccgc cattcggctg ttaatgggcg aatcttcagt actggtatta ggtggacaac
    33541 gcgcgctgcc taaacggctg gaagaagcgg gttttgcgtt tcgctggtac gatttagaag
    33601 aggcgctggc ggatgtcgtt cgctgatgtg gtttacagca aacatccgcc agttaactcc
    33661 ctgtgttaca ggattagtgg ctttgcgcga taagatcgtc tggtgaaagt cgggtcacca
    33721 tcataactaa ctctctgtct aaacctctat ccagcatctc ctgagcaata cgcagggctt
    33781 cttcgtgttt gccctgcatt gcgccttctt cacgtaatct gtcagcaatg gtcatcagtt
    33841 tctccttttc ttgtggtgcg cgttctgtta tctcaccaat aaatgcacgg aaacgctggg
    33901 catcccctgt ttgtaatacg taattaaaca gggcttttag ctgtctgtca ttagtgttcc
    33961 ctgtaactaa cagcgaaaca atttggtcga ctaatcccaa cagatcgcgc tgacgaatat
    34021 gtttctgaat taactccaac agcgccattt tgcggtgttg cataatctcg tcatccggca
    34081 ccacggtaat atccaccaac ggaaacgctg atgaatatat tttgcgggct atagcaggct
    34141 cggcaaattc atcaagccag cagagtgaat aaggataagg acttctgcaa ccatgataaa
    34201 acagcatcgg gatcaccaat ggaagctctt tatagcccgc atcaagatgg ttttgcattg
    34261 ccgcaatgga ataacgcatc atgcgaaaag ccattaattc ttccggctta ctttggtgct
    34321 ctatcactac ataaatataa cccactccct cctgcgtttt cacagaccac aagaggtcgg
    34381 aataatattg ccgcaggtct tcatcaataa aactgtttgg ttctagttta agcgtcgtta
    34441 aatcacacag tttgcgcagc ggcgcgggaa gatgaatatt aataaaattc cgcgcggtgt
    34501 ctggatggcg taaaaaagat ttaaataccg catcatgcgg cgtggaagtt gtcgatattg
    34561 tcatggcgtt ccgtcacccc tcaaatttaa gatgacgcga caataaccac gattaacacc
    34621 caatgcagca ggcagttttt atctttggac agcgaattca gaagtatttt tagcggactg
    34681 caacttttac atttcagcgg aagctgactc gcaaattccc cttgaaaata ctccggcagc
    34741 gttagccacc ggagaggaaa ttatttcagc gatcccttaa ggaactgttg cagacgaggg
    34801 ctttgcgggt tgccaaataa ctgctccggc gcgccctctt cttctatttt cccctgatgg
    34861 aggaaaatga cgtgagtaga aacatggcga gcaaagccca tttcgtgagt cactaccacc
    34921 atggttttcc cctcttctgc cagttgctgc ataatacgta acacttcgcc taccagttca
    34981 ggatcgagcg ccgaggtagg ttcatcaaac agtaaaactt ctggttccat cgccagcgcc
    35041 cgcgcgatag aaacacgctg ttgctgaccg ccggaaagat gcaccggata tttcccctgc
    35101 gcacgttcgt ctatcccgac tttcgccaga tacttcaccg cccgctcgcg cgcttcctgc
    35161 ttgctcaggc ccaacacctg aatcggcgct tccatgacgt tttccaacac cgtcatatgg
    35221 ctccagagat tgaagtgctg gaataccatc gtcaggcgtg tgcgcagtaa gcgcagttga
    35281 tttttatcgg cgactttgag ttgaccgtct ttgtcgcgca ccagattgat cgtctggcca
    35341 ttgaccacga tcgatccttc actcggtttt tcgaggaagt taatgcagcg cagaaaggta
    35401 cttttccccg atcccgacga tccgatgatg cttattacat ctccggcatt cgcttgcagt
    35461 gagacccctt tcagcacttc gtgttcgcca tagcgtttgt gcaaatcgat aacgtttaat
    35521 ttattctcgg acatcgtatt ctcagtgcgt tgaagaaggt ttcacatgct gcaaccagcg
    35581 tttttccgct ctgcgaaaga ggctgatcag gacataagag atgattaaat agagcaccgc
    35641 ggcaatgccg aaggcggtaa aaggttgata cgtggcggcg ttaatatcgc gggctatttt
    35701 cagcagatcc ggcacagtgg ccgtaaacgc cagcgcagta gagtgcagca tcaggatcac
    35761 ttcgttgctg tatgccggta acgcaatacg cagcgcagaa ggcaaaataa tgcagcgata
    35821 cattttaaag gtcgagaagc cataggctct ggcggcttca atttccccat gcggaaccga
    35881 acggattgcc ccagcaaaaa tctcggtagt gtaagcgcag gtgttaagcg tcagcgccag
    35941 cacggtacag ttcaggccac tgcggaagaa agcgttaagg aattcggttc ccttaacaat
    36001 ctcaagcgtg tacatgccgg aatagaacac cagcaactga acatacagcg gcgtaccgcg
    36061 aaaaatatag gtaaataacc agattggaaa ctggatgtat ttattactgg agacacgacc
    36121 aatcgccaga aacagcgcca ggactccgcc tatcactacc gacaaaataa gcagccacag
    36181 agtgatcgcc acaccagtaa agcgataacc gtcggtccac agcagcggtt tccagtattc
    36241 atgtaagatt tcgatcacag gtcagccctc ttcacaccca cggagtagcg gcgctcaagg
    36301 aacagcagca caccattgga aacggtggtg aaaaccaggt aaatcacgcc acagacgatg
    36361 gcgaaataga acggttccca ggtacttttg cctgccagct gcgtggcttt gaccacatct
    36421 tccaggccga gtaacgaaac cagtgcggta gatttgagga tcacctgcca gttgttgcca
    36481 atgcctggca gggcgtaacg catcatcgcc ggaaacatga tccgccgaaa cacttgcccg
    36541 cgagtaaaac cgaacgccgt cgccgcctct atatgccctt tcggcactgc cataaacgcg
    36601 ccacggaacg tttcggtaaa ataagcaccg tagataaaac cgagagtgat aataccagcg
    36661 accatcggat cgatatcaat ctgcccgacg cccatcgcct ccgtcaccat gtttagcgca
    36721 atctgcaaac catagaaaat cagcagcatc aacaccaagt ccggcacgcc tcgaatcagc
    36781 gtggtgtacc cttcgaaaat cagccccgaa agccgatttt gcgagagctt accgccagcg
    36841 ccaattaaac cgatgattac agcgagcact acagagctga tagccagctc cagcgtgacg
    36901 agcgcaccct gtaaaataac acctgaaaac ccatacaaca tgctgcctgt cctgtcgtgt
    36961 gtggtgaatt accgcctctt gtctccctcc ggttacccga agggagcggg ctttctttca
    37021 ccgatggcga ttaaccacca taaacatcaa aatcgaagta ctttttcgct aatttctcgt
    37081 aagtaccgtc agcgcgcatt tcggcaaagg ctttgttcag tgcctcgcgc agttcgttat
    37141 cttctttacg caggcccatg ccggtcccta cgccaaacag tttttcatct ttaacagacg
    37201 gaccaccgaa tttgtaatct ttaccgacgg gttgtttgag gaaaccttcg ctggcagcga
    37261 cctcatcctg gaacgcggca tcaatacgtc cggcagtcag gtcagaataa atgttgtcct
    37321 gcccctgata cgagacgatt tcaatgcctt ttggtgccca gtgttcatta ccgaacgtct
    37381 cctgggtggt gccctgcaat acgcctaccc gtttgccttt cagcgactcg actgtcggct
    37441 gaatgtcaga atttttcgcc accaccagac gagaatcggc agcgtacagt ttgtcggtga
    37501 aggcaatttc ttgctgacgt ttttccgtaa tggaaagcga tgacatgatg gcgtcaatct
    37561 tcttcgcttt taaggacggg attaacgcat ccagcgggtt ttcgacaaac gtacattgcg
    37621 tattgatgcg cttgcacaac tccttcgcca gctcgatatc gaaaccaacc agttcgcctt
    37681 gtgaattttt tgattcaaat ggcgcatagg tcgggtcggt accgatgcgg atgttttgcg
    37741 gaattgcagc aagtgccgca gttgcgctgg agaaggccag aaccagagag agcgatagca
    37801 ccagtttttt cataatgtcc tcaactgaca gtctttttat agggaatatt tacaggtttg
    37861 ttacacttat cgtgccataa aattgaccat caaggcaaaa tatcccgccc cgttagacgt
    37921 attatttttc attaaaaatg cttaagtatg catttcaatg caccacgata gtgcaacaaa
    37981 cctggcgtgc accatgatga tcattccatc aggtacagct tcccagcgac gtatcagtca
    38041 ccgtagacat taaagtcgaa atactttttc gccatcttgt cgtaggtgcc gtcctgacgc
    38101 agctcgccaa gcgccttatt gaaggcagcc gtcagttcag cataatcttt acgtagccct
    38161 acaccggtgc catcaccgaa gtattttttg tcttttactg atgagccagc aaaagcgaaa
    38221 tctttaccag caggttgctt gaggaatcct tcgctggcag caacttcatc ttgtaacgca
    38281 gcatccagac gtcctgcagc cagatcggaa tagaccaaat cctggttggc ataggccacc
    38341 acatccacgc ctttactacg ccaggtctcg ttagcgtaag cttcctgggt tgatccctgc
    38401 agcacaccaa catgtttacc tttcagtgaa tccagcgttg gctgaatcgg tgaacctttg
    38461 gcagcaatca aacgagaatc tgcggcgtac agcttgtcgg agaaggcaat ctcctgctga
    38521 cgtttatcgg taatggaaag cgacgaaata atagcgtcga tttttttcgc tttcagtgag
    38581 gggatcagcg cgtcaaagtc actggcaacc caggtacatt tcacctgcat ccgtttgcac
    38641 atctcgttac cgagatcgat atcaaagcca acaaaatcac ctttagcatc tttcgacgag
    38701 aacagcgcat aggtggtatc ggttccgata cgtaccgtct ccggtagcgc cgcatagctg
    38761 gaagccgctg cggagagacc gactaataaa gacagagcga gaatcgactt cttcatacat
    38821 aaccctcaag tgaaaatggc ttttttatgt tgtgtattgt gttgtgtgtt tgcaggctct
    38881 ttcatgcagg tcttatgcca tcttgacgac aacagtaaca ttcaacgtta aatatgttaa
    38941 taagacattg cattattgtc ctgaagttga agatagcagg tatagcggtt gaatcgcagc
    39001 gtttcgattg aatggcagaa acaaaatgtc gaggatttga tcgcggttgc aaaattgccc
    39061 tgaaacaggg caacagcgga gttatgcgcc ctgccagcgg gcaaagagat cttcaggaag
    39121 ggttattgca aactggtcaa gaacacgatt aaccgtctga tttatcacat catcaaggga
    39181 ttgcgggcga tgataaaacg ccggaacggg aggcataatc accgcaccga tttctgccgc
    39241 ctgagtcatt aaacgcagat ggcctaagtg caatggtgtt tcacgcacgc agagcaccaa
    39301 cgggcgacgc tctttcagca ccacatctgc cgcacgggtc agtaagccat cagtatagct
    39361 atggacaatg ccggacaggg ttttgattga acagggtaaa atcaccatcc ccagcgtctg
    39421 gaaagaaccg gaagagatgc tggcggaaat atcgcgcgca tcgagcgtga catcagctaa
    39481 tgcctgcact tcgcgcaggg aaaaatccgt ttcgagggat aaggtctggc gcgccgcctg
    39541 gctcatcacc agatgcgttt cgatatctgt gacatcgcgc agaacctgta ataagcgcac
    39601 gccataaatc gcgccgctgg caccgctgat gcctacaatg agtcgtttca taaatagttg
    39661 ccctgtcaga cttgcaggca gactttgcag tatttcggcg ggagttgcaa gtcagggtgc
    39721 cagaccggca ccctcagcga aggcatcatc cttcgttatg catttcgaga ttttccactt
    39781 cgttctgacg ttgcactgct ttggcgtcat cattacgtaa cgtatcgagg aaatcgaggt
    39841 agccctgatc aacatctttg gtgacgtaga cgccgttgaa caccgagcat tcaaactgct
    39901 ggatatccgg attttcagcg cgaacggcgt cgatcagatc gttcagatcc tggaaaatca
    39961 acccgtcagc accgatgatc tggcgaattt catcaacttc gcgaccgtga gcgatcagtt
    40021 ccgtggcgct cggcatatca ataccataaa cgttcgggaa gcgaatttcc ggtgccgcag
    40081 aagcgaggta cactttcttc gctccggctt cgcgtgccat ctcgataatc tgctcagaag
    40141 tggtgccacg gacgatggag tcgtcgacca gcaggacgtt tttatcgcgg aactcggcgc
    40201 ggttggcatt cagtttacgg cgcacggact tacgacgcag ctgctggccc ggcatgataa
    40261 aggtgcggcc aacatagcgg tttttaacga agccctggcg gtacggtttg cccagaatac
    40321 gagcaatttc cagcgcgata tcacacgagg tttctgggat cgggatcacc acgtcgatat
    40381 ccagatcttc ccattcgcgg gcaattttct cgcccagttt cgtgcccata ttcacacgcg
    40441 cgctgtaaac ggaaattttg tcgataaacg agtccgggcg ggcaaagtat acatactcaa
    40501 acaggcacgg attgctgacc ggattgtcag cacattgacg ggtaaacaac tgcccttctt
    40561 cagtgatgta aatcgcttcg cccggcgcga cgtcacgcag gaaatcaaag cccagcgtat
    40621 cgagcgctac gctttcggaa gcgaccatat attctgtacg gttctcgtca atatcacgtt
    40681 ttcccagtac cagcggacga atcccgtttg gatcgcggaa agcaaccata ccgtggccga
    40741 taatcatcgc cacacaggca tacgcgccgc ggattaagcg gtttgtggca gcaatggcag
    40801 cgaaaatatt gtcggcttcc agcgggtagt ggcggaagtt gtccagctcg ctggcgaaga
    40861 tattaagcag aatttccgag tcggaagtgg tgttgatgtg gcggcgtttt tcttcaaaca
    40921 gttttttacg caactcgtga gcgttggtca gattgccgtt gtgggcaagc gtaatgccat
    40981 acggggagtt aacgtaaaac ggctgcgctt cagaggcgct ggagctgcca gccgtagggt
    41041 aacgcacatg accaatgccc atattgccct gcaaacgctg catatggcga gcttcaaata
    41101 catcgctcac cagcccgttc gctttacgca aacggaagca gttattggca tctatggtga
    41161 tgatgccggc ggcatcctga ccgcgatgct gaagcaccgt taaggcatca taaatcgact
    41221 ggttaaccgg cataacaccg gcgataccga caataccgca catacgtctt tttcctcgtt
    41281 aagccacatc tcagagcact tacgctctgg gcaagaaact tgacgagctt tgcagataat
    41341 caaaaaacca tctgatgata aaactgaatt gcgggatcag ctgtgatttg ctccagtctt
    41401 cacttttcga caccccggta aaggagtcga gaaagaagag aatggcggca acaatcaaca
    41461 caccgcgcaa cgcaccgaaa cagacgccca gcacccgatc ggtgcctgac aaccccgttt
    41521 tctccaccaa ctggcctatc acgaagttca cgatagcacc aacgatcagg gtagcgataa
    41581 acagtaccgc gatggcaatc ccatttcgaa ccagttcgtc ttcaaagccc gtaaaccaga
    41641 ctgacaggta agtgtagtaa tgactggcaa caaagaaagc acaaccccat gtcaccagcg
    41701 ataacgcttc acgaacaaag ccgtggatca ggctaaccag agaggaaaaa gcaatcaccg
    41761 cgattatggc gtaatcaatc cagaccatat gtgtcccacg attttacgcc ctgtcatcct
    41821 gttcggggcg cattctaaca gaaaaagaaa acgtttgcgt agggatttcc ttcccgcgca
    41881 tcaataaaaa tggcgctgaa aaaatattca acgccatcga ctttttatgc ctttgcggca
    41941 tcgtgcaatg cgtgtcggat gcggcgtaaa cgccttatcc gacctacggt tctaacactg
    42001 cccaagcctg ataagacgcg ccagcgtcgc atcaggcaag actgtattaa ttcggcgtat
    42061 agcccattac cacgccactt aagccagaaa gttgcttcaa ctcacccagc gaacctttca
    42121 gcttatcttt cgaggcatcc ggcccaacca gaatacgggt aattttaccc tgcactggcg
    42181 tggatggcga cgtataaacc cgataaccgg caccgcgcag cttaccgaca atctcattca
    42241 ctttatcggc atttttcagc gcacccagtt gcacaacata ggctttaccc gttggcgctg
    42301 ctttttcttc cacgaccggc ttcggttctg gcttcggcgc aggtggtact tcaacctttg
    42361 gtttaggcgg ctccaccggt ttcggcttcg gtggggcgac cggtgcaggt tccggttcaa
    42421 actcggtgtt attggctgca atagtggccg gatcgagcga cggtgctgcg gcatcacctg
    42481 cccgcacctc ttccgctgcg ccttccggcg gctgcgtcgg caacgcctgg gtggcggctg
    42541 gcatcatatc aggctcatca cgatcgcccg ctttcggcac cagcgggata gccgcgaact
    42601 catcctgata atgttttttc tgcccgtcca gcagccctgg aagtacaatc acccccagcg
    42661 ccaccagcac gatcgtgccc actaaccgat tctgaaactt acttgccacc gcttctcctc
    42721 gcgtcaatca cttccatgac atgtgcgacc gtgtggaaag aaccacacac cagcacggtg
    42781 tcttccgctt tagcgtccgc cattgcggca tcccatgcct gcgcaacgct atcaaatgat
    42841 ttgccgttac ccaaatgctc aagcagttgt tctgccgtgg caccgcgcgg cccttccagt
    42901 ggcgcacaat accagtcatc aaccacgctt ttcaaccagg ccagagttcc ggcaatatct
    42961 ttatcatgta gcataccgat aaccgccagc acgcgcccgt ttttcggtag cgctttcata
    43021 cgcccggtga gatattccgc cgcatgtgga ttatgcgcga catcaaaaat aacgcgtggc
    43081 gactcgctca caatctggaa acgtcccggc aaaattgcgc tggcaatccc gtcgcgaatg
    43141 gcattttcac tgacttccag cccgctggca cgcagtgccg ccagcgccgt tgcggcattc
    43201 ggttgcggga caagcggcaa cggcagattt tccagcgtgc cgtgagcatc gctaaacgcc
    43261 cagtcatgat cggtgacgga atagttccac tcaacgcccc gacgttgtaa cagtgcacct
    43321 ttttcctgcg ccacatcagc aatggtagaa ggcatttccg gctcaccgac aattgccggt
    43381 ttttcgctgc ggaagatgcc tgctttctcg agaccaatac tttcgcgatc tggacccagc
    43441 cagtcggtat gatccagcgc aatactggtt actaccgcga catcggcatc gacaatattg
    43501 gttgcgtcca gacgaccgcc cagccctact tccagaatca ccacgtcaag ttgtgcctgc
    43561 ttgaacagcc acaacgccga cagcgtaccg tactcgaaat aggtcaggga aatatcaccg
    43621 cgtgccgatt caatctccgc aaaagaggcg gtgtgggccg attccggcaa ttcctggccc
    43681 tgcacacgta cgcgctcggt ataacgcacc agatgaggcg aactgtagac gcccactttg
    43741 taccctgccg ccatcagaat cgactccagc gtacggcagg tggtgccttt gccattcgta
    43801 cccgcaacgg taaacacaaa tggcgctggt ttcaggacgc caagacgcgc cgcgacctgg
    43861 ctcacgcgct caaggccgag atcgatagtt ttactgtgca ggttttccag ataagaaagc
    43921 cacgaagcca gaggcgacgc ggcttgagga gtgcgtttga taatcatggt atccgctgat
    43981 tcgttacggt gagaatagca aaagggcaga gccagtggcc ctgcccttat cagttatcag
    44041 gcctcaggtt cctgatccgg taccgggggt accactacgc cttcacgcgg cgcttcagga
    44101 ttcggcgctg gcagattcat caacttcgcc agaatgctcg ccagtttcag gcgcatttcc
    44161 ggacgacgga cgatcatgtc gatcgcgcct ttctcgatca ggaattcact gcgctggaat
    44221 ctaggcggca gtttttcgcg aacggtctgt tcgataacac gcggaccggc aaagccgatt
    44281 aacgctttag gttcagcgat gttgagatcg cccagcatgg cgaaacttgc agaaacaccg
    44341 cccatcgtcg ggtcggtcag cacggagatg tacggcaagc cgcgctcctg catttttgcc
    44401 agtgccgcag aggttttcgc catctgcatc agcgacatca gtgcttcctg catacgtgcg
    44461 ccaccagagg cggagaagca gatcagcggg cagttatctt cctgcgcctg ctcaacggca
    44521 cgcacgaaac gtgcacccac aacagacccc attgaaccgc ccataaaggc gaactcgaat
    44581 gccgcagcga caaccggcat tccatacaga gtgcctttca tcaccaccag cgcatctttt
    44641 tcgccggttt ctttctgcgc agatgccaga cggtctttat acttcttgga gtcacgaaac
    44701 ttcagctcat ctttcggctc aagctcgcta cccagctcca caaggcttcc ttcatctaac
    44761 aggctatgca ggcgattacg cgctgtcata cgcatgtgat ggtcacactt cggacagacc
    44821 tcaagattac gttccagctc agcgcggtat aaaacctgac cgcagctatc acacttagtc
    44881 cacacccctt caggaatgct cgccttgcgg gtgggagtaa tgttgctttt aattcgttca
    44941 atccagctca ttagggacct ttctgtctga acctggttcg atgccagttt tatctttggg
    45001 gacgcataat gccatttttg cccccaacag accatgaatg ttgcacatta aaacataaca
    45061 gcccgaaact ttggataaaa aagtggtcga accgcggagt tactttttat tttgcggcgc
    45121 gtgccgcagc gcgtttgtga cggattattt cgatgacgcc cggcaaaata gaaaccacaa
    45181 taatcccgac gatcagcagc ttaaggttat cctgaaccat cgggattgta ccgaagaaat
    45241 agcctgcgta ggtaaaaagc agtacccaca acagtgcgcc gatcacgtta taagcagcga
    45301 aatgacggta cgacatgtgg cccattcccg caacaaacgg ggcgaacgtt ctgacgatcg
    45361 gcacaaaacg ggcgagaata atcgttttgc cgccatgttt ctcataaaac tgatgggttt
    45421 tgtcgagata actgcgacgg aaaattttcg aattgggatt actgaataac ttttcaccga
    45481 acaaccgccc gatagtgtaa ttgaccgcgt cacccacaat cgcggcgatc agcatcagca
    45541 ctaccatcat atggacgttg agatcgttgg tttccagtga tgccaacgct ccggcaacaa
    45601 acagcaatga atcacccggt aaaaacggca ttaccaccag accggtttca cagaacaaaa
    45661 ttaagaacaa aatggcataa acccagacgc cgtactccgc gactagctct gccagatgca
    45721 cgtcaatgtg caggataaaa tcgatgagga aataaatcag gtccatattt gcctatgcct
    45781 tgtactcgtc atctttcagg ctgtaactac gttggctaca gcctgaaatg ttccgagcat
    45841 tattctcgtt agtccgccag aaatagcggg cccattggcg gttttggaag atcataccgg
    45901 tcagggtaat ccaccgcgac cagatacagc ccttccgctt ttgccgttgc tgccgccagc
    45961 gttctgtcct ttgccgccag caactctgct atccagctct ccggctggtt gtgggcaccg
    46021 acttccatca ggctgccgac aatattcctg accatatgat gtacaaaggc attcgctttg
    46081 atatcaacca ccacataagg accgtgacgc gtgacattaa tatgcataac gttgcgccac
    46141 ggggttcggg actggcactg caccgcacgg aacgaggtga aatcattctc gcccagcaag
    46201 cattgcgcag cccgatgcat ccgttcagcg tccagcggtt cgtaaaaatg ggttacccct
    46261 ttactcagta ccgccgggcg cagccgatga ttgtagatga tgtagcgata acggcgagcc
    46321 gtggcgctaa atcgggcatg aaaatcatca ggtacagctt taacccaacg cacggcgatg
    46381 tcaccaggta aattcgcatt tacgcccaag gtccacgccg cgtctttgcg ctgcgcggtt
    46441 gtttcgaaat gcaccacttg cccggtaccg tgtacccctg cgtcagtacg cccggcgcag
    46501 aagacggtga tgggttcgtt cgccacctgg gagagcgcct tttccagctt ctcctgcaca
    46561 ctgcggactt cattctgccg ttgccagccg taatacttac tgccgtcgta ctcaatgccc
    46621 agcgcaattt tataaactgg cagttgttgc tggtcggaca ttagtacaga tactcctgca
    46681 ccagtttctc ggcgattttt actgccatca gcgcgccgcc aaagcgaacg ttatcggcca
    46741 ccgaccagaa ctggacttgc tccggcatac cgtagtcatt acgcacgcag ccaacagaaa
    46801 gatgcggcgt acccgaagca tcccctacct gagtcgggaa ttcgttctct tcagagagca
    46861 caatatcttc gccctgagca aacgcatcac gcgcttcttc tgccgccagc ggacgcagtg
    46921 cttcaaagtt gaccatctgg gcatgaccgt agaataccgg tgcctggacg acgcttgccg
    46981 aaatcatcag cccttcgtcc tgcaggattt tgcgtacttc gtcaacgata cgacgttctt
    47041 cacgcacgct accttcgcta tccggcaaca acggcagcat attgaacgcc agttgacgac
    47101 cgaagaaatc ttcttcgtca atcggaatgc cgttgagcaa tttcgcactc tgccccgcta
    47161 acgcatcgac cgcttttttg ccctgggcgg aggctgaaat caggctggta acactgatac
    47221 gcgacaaacc gccctgatcg attaacggtt tcagtgccgc cagcagctgg ctggtcagac
    47281 tgtctggtac ggcgatgaca ttccggttgc ggtaatcggt cagtacaaac gggtttactt
    47341 ccggcaccac cagcggtacg tcgggttcga gagcaaacag tccgctgctg tcgatcacca
    47401 ggcaacctga gttggtcgct tcttcaaccc aggcagcggt agcttctttg cctgcgacaa
    47461 aaaatgccag ctgcgcctgc gtccagtcga attcagcggc atcctgcacg gtgattgttt
    47521 taccaccaaa gcgcagttgt tcgcctgcgc tttcgttacg tgccagtgca taaatttccc
    47581 caaccgggaa ctgacgttca gccagcgttt caagcagggc ttcgcccata gcgccagttg
    47641 cgcccaggac ggcaatgttc cagccttcag acatggtggt ttactccaga aatagcaaag
    47701 ctccctgcca atgttacagc agagagcatg aagaagagat taacgtgccg gatgatgaac
    47761 ggcgttaaaa cccagtttac acagcaatga tgccgcactg gcgtcatcac aaattacata
    47821 cagagatgac cattcacggc gctcaagata gtttttgcgc agtttatcga actcacccgg
    47881 tatcccggcg actttacgca gcggtgcgtc atcgcggcgc acatcataca ccaaatgcac
    47941 cagccttttc agcgtcggtt gatcgagcgg accatgcagc gtaatgcggc caaactcagg
    48001 cgcgggcagc aacgtatcca gcgcaacgtg ttgttcatgc ccaataaact tgctataggc
    48061 ttcaaacact tgcgtcgtgc cacgcgcttt accctccagg gtatagcctg cgatatgcgg
    48121 cgtgccgata tccacttttg tcagcaattc cacattaagt tccggctcgc cttcccagac
    48181 atccagcacc acgcttaact tctggccttc attcaggcag gtcagcagcg cggtattatc
    48241 gacgactgcg ccacggcagg cgttaatcag aatcgctccg ggcttcaggc tacggatcag
    48301 tttttcatcc gccagatgta gcgttttgta cggaccatct ttaaagagtg gcgtatggaa
    48361 agtcagaata tccgcgcgct ggactaactc atccagcgag cggaaatcac cctcatcccc
    48421 acggtcggcg cgaggcggat cgcaaagtaa ggttttaatc cctaacgctt ccagtcgcgc
    48481 ctgcaatcga cgtccaacgt taccaacgcc cacgatcccc accgtacggt cgtgcagtga
    48541 aaatccatcg cgttcggcaa gcatcagcag ggaggagaaa acatattcca ccaccgcaat
    48601 cgcattacag ccaggtgcag cggaaaaacc aattcccgcc tgcttcaacc atgcttcatc
    48661 gacatggtcg gtccccgcag tggcagttcc aacaaattta atgggttttc ctgccagcaa
    48721 agattcattc actttcgtga ccgaacgcac catcagcgca tccgcgtctg ccagttgagc
    48781 gacggggatt ggacgcccgg gaaccgcgat cacctcaccc aaacggctaa ataagtcgcg
    48841 ggcataaggc atattttcat caacaaggat tttcacgttt gtgttacctg tatgagacga
    48901 gagttaaccg gacaagtatg ccataatctc gcggccaggc atacttgcga agatttcagg
    48961 tataaggata cgtaatgata caacctattt ccggccctcc tcctgggcag ccaccaggtc
    49021 agggagataa cctgccgtct ggcgcgggca atcagccttt atccagtcag caacgtactt
    49081 cgctggaaag cttaatgacg aaagtgacct cactgacgca acaacaaaga gcagaactgt
    49141 gggcgggtat caggcacgat attggtctgt cgggagattc accgctgctt tcgcgtcact
    49201 tccctgccgc tgagcataat ctggcgcaac gtctgctggc cgcgcaaaaa agccattctg
    49261 cccgccagct tttagcgcaa ttaggggagt atttacgtct ggggaatcat cgtcaggcgg
    49321 tcacggatta tatccgtcat aactttggtc agacgccgct gaatcagctc tcaccggagc
    49381 aattaaaaac cattctcacc ctgttgcagg aagggaagat ggttattccg caaccacagc
    49441 agcgcgaggc gaccgaccgt cctttattac cggcggagca caatgcgcta aaacagctgg
    49501 tgaccaaact tgcggcggca acgggggaac ccagcaaaca gatctggcaa tcgatgctgg
    49561 aactttccgg ggtgaaagat ggcgagttaa ttccagcgaa actgtttaac catctggtga
    49621 cctggctaca ggcgcgtcag acgctaagcc agcaaaatac gccgacgctg gaatcactac
    49681 agatggcgct aaaacaacct ttagatgcca gtgaactggc ggcgttatcg gcatatatcc
    49741 agcaaaaata tggcctttct gcgcaatcat cgctttcttc tgcccaggcc gaggatattc
    49801 ttaatcagct ttatcaacgg cgggttaaag ggattgatcc gcgtgatatg caaccgctgc
    49861 ttaatccttt tccaccgatg atggacacgt tgcaaaatat ggcaacgcgt cccgcgctgt
    49921 ggatactgtt agtcgcgatt atcctgatgc tggtctggct ggttcgttaa ccccgacgaa
    49981 atgacagtat cgtgacaata atacccagca ccgcagagat cgccccggca agaaataccg
    50041 aagagtaacc aaacgtggtc gccagcattc ccgccagcgg cccggagacg ccgagggcga
    50101 tatcctgaaa cgcggcgtaa ccgcccagtg cggtgccgcg aacttgtgag gggacgcgtt
    50161 taaccacctc cacgcccagc gcaggaaaga taagcgaaca tccggctccg gttaacgccg
    50221 cgcccgctaa tgcgacccat gcacctgggg cttgccagag cagcaacaag cccaccgttt
    50281 ctacaagcag agagacaatc gccactttca cgccgccaaa acggtccggc atccagccaa
    50341 acatgacgcg catcacgaca aatgcgccgc caaacgcggt aagagtaaag cccgccatcg
    50401 cccatccttt gctggcaaag tagagcgaaa cgaaagtccc gataaccgca aaaccaacgc
    50461 cttgtagtgc cagccctaac cctggtttcc agataagccc gacaacgctc cacagcgatg
    50521 gacgttctcc cgccagggcc ggtactttgc gcactgtgcc gttacaggcc cacgccagta
    50581 agggtaatac cattgtggtg atcgccagtg cggcaaaacc gtaatggcta tgaatcaaca
    50641 ggccaagcgg cgcaccaaca gcgagggcac cgtaaatcgc cattccgttc catgacatca
    50701 ctttgccaga gtgttttggc cctacgatgc ctaaccccca ggtcagagcg cctgtcagta
    50761 actggctttc accaaaccca agaatcaaac gcccgatgac caacagggca aatttgaacg
    50821 gtgcggagac aggcaaaatc gccgccagca gcaacgcgcc gccagccaga ccacaagcta
    50881 acatcccctg aagcgccgaa cgttttgcgc catattgatc ggccagtcgc ccggcgtaac
    50941 cacgcgtcag caccgtagcc agaaactgaa tcccgacggc aatgccgacc atggtattgc
    51001 catagcccag atcatgatga acaaacagcg ggataaccgg caacggcaac cctacggtca
    51061 tgtaggtgag aaaaaccgca aaagagatgc ggaagagcga aaaactggca gaagatcgtg
    51121 tttcggtttg gcttacagca gtcatgcatt actccagaat gcagcgcaag gcgaggagta
    51181 tccccgtctc atctctctgg tttcagggtt acggtgcgtt ggcaggattt aacgcgtacg
    51241 tcttttcaga aggaaatcga caaagcggga agtttgccta gaactggtgg cgattgtcaa
    51301 tgatgtgaaa aagggaacca tcaggttccc ttttgcgtta gtgccggagg ccgcattggc
    51361 gcgtaacgtc ggatgcgacg ctggcgcgtc ttatccgacc tactccggcc tactgcgaat
    51421 taatcttttc agcttgcgca ttaccagcgt ggcgttggtg ccgccgaagc cgaagctgtt
    51481 agacataaca gtggtcagtt cgcgatcggt cgtttcggtc acgatgttca gacccgcagc
    51541 ctgctcgtcc agctcttcaa tgttgatgct cggggcgata aagccgtgtt ccagcatcag
    51601 cagagagtag atagcttcct gcacacccgc agcgcccagt gagtgaccgg tcatggcttt
    51661 ggttgcagaa atcgccgggc tcttatcgcc gaacacttca cggatagctg ccagctcttt
    51721 cacgtcgcca accggagtcg aagtaccgtg agagttcagg taatcgattg gggtatcaac
    51781 gccatgcatc gccatctgca tacaacgtac tgcgccttcg ccagacggag caaccatgtc
    51841 tgcaccatca gaggttgcgc cgtagccaac gatttcagca tagatgtaag caccacgcgc
    51901 cagcgcgtgt tccagctctt caaccactac cataccgccg ccgccagcga taacgaaacc
    51961 gtcacggtga gcgtcgtaag tacgggaggc tttttccggg gtgtcgttgt atttagtaga
    52021 tagcgcaccc atcgcgtcga attcgcaggc catttcccag cacagctctt cgccgccgcc
    52081 agcaaacacg atgtcctgtt tgcccagttg gatctgctct actgcgttac cgatacagtg
    52141 tgcggaagtc gcacacgcgg agctgatgga gtagttaacg ccatgaattt taaacggcgt
    52201 ggcgagacag gcagaaacgc cggatgccat cgctttagtg accacatacg ggccaaccgc
    52261 tttcaggccg cgcgggccgc gcattgcgtc agcgccgaac acctggaaac gcggggaacc
    52321 gccgccggaa cctgcaatca ggccaacgcg cgggttattc tggtaagctt ccggagagag
    52381 gcccgcatca gcgattgcct gctccataga aaggaatgca taaatggatg cgtcgctcat
    52441 aaagcgcaca actttgcggt caatgaggcc agtggtatcc agttttatgt tgccccagac
    52501 gtggctacgc atgccggaat ccttcagctc ctgagagaaa gtgatccctg aacgtccttc
    52561 acgcagagat gccaggactt cctgctggtt attaccgatg ctggaaacaa tgcccaggcc
    52621 agtaatcact gcacgtttca ttcaatacct ctgtaagtcg cacatagagt aagtttcgaa
    52681 tgcacaatag cgtacacttg tacgccgaac aagtccgatc agccatttaa tagagatatt
    52741 tgcgcagcct tacacacatc gctaagatcg agccaccgcc tgtaagacga gtaacttacg
    52801 tgaaacacta ctccatacaa cctgccaacc tcgaatttaa tgctgagggt acacctgttt
    52861 cccgagattt tgacgatgtc tatttttcca acgataacgg gctggaagag acgcgttatg
    52921 tttttctggg aggtaaccaa ttagaggtac gctttcctga gcatccacat cctctgtttg
    52981 tgatagcaga gagcggcttc ggcaccggat taaacttcct gacgctatgg aaggcatttg
    53041 atcagtttcg cgaagcgcat ccgcaagcgc aattacaacg cttacatttc attagttttg
    53101 agaaatttcc cctcacccgt gcggatttag ccttagcgca tcaacactgg ccggaactgg
    53161 ctccgtgggc agaacaactt caggcgcagt ggccaatgcc cttgcccggt tgccatcgtt
    53221 tattgctcga tgaaagccgc gtgacgctgg atttatggtt tggcgatatt aacgaactga
    53281 ccagccaact ggacgattcg ctaaatcaaa aagtagatgc ctggtttctg gacggctttg
    53341 cgccagctaa aaacccggat atgtggacgc aaaatctgtt taacgccatg gcaaggttgg
    53401 cgcgtccagg cggcacgctg gcgacattta cgtctgccgg ttttgtccgc cgcggtttgc
    53461 aggaagccgg attcacgatg caaaaacgta agggctttgg gcgcaaacgg gaaatgcttt
    53521 gtggggtgat ggaacagaca ttaccactcc cctgctccac gccgtggttt aatcgtacgg
    53581 gcagcagcaa acgggaagta gcgattatcg gcggtggtat tgccagcgcg ttgttgtcgc
    53641 tggcgttatt acggcgcggc tggcaggtag cgctttattg cgcggatgaa gcccccgcgc
    53701 tgggtgcttc cggcaatcgc cagggggcgc tgtatccgtt attaagcaaa cacgatgagg
    53761 cgctaaaccg ctttttctct aatgcgttta cttttgctcg tcggttttac gaccaattac
    53821 ccgttaaatt tgatcatgac tggtgcggcg tcacgcagtt aggctgggat gagaaaagcc
    53881 agcataaaat cgcacagatg ttgtcaatgg atttacccgc agaactggct gtagccgttg
    53941 aggcaaatgc ggttgaacaa attacgggcg ttgcgacaaa ttgcagcggc attacttatc
    54001 cgcaaggtgg ttggctgtgc ccagcagaac tgacccgtaa tgtgctggaa ctggcgcaac
    54061 agcagggttt gcagatttat tatcaatatc agttacagaa tttatcccgt aaggatgact
    54121 gttggttgtt gaattttgca ggagatcagc aagcaacaca cagcgtagtg gtactggcga
    54181 acgggcatca aatcagccgt ttcagccaaa cgtcgactct cccggtgtat tcggttgccg
    54241 ggcaggtcag ccatattccg acaacgccgg aactggcaga gctgaagcag gtgctgtgct
    54301 atgacggtta tctcacgcca caaaatccgg cgaatcaaca tcattgtatt ggtgccagtt
    54361 atcatcgcgg cagcgaagat acggcgtaca gtgaggacga tcagcagcag aatcgccagc
    54421 ggttgattga ttgtttcccg caggcacagt gggcaaaaga ggttgatgtc agtgataaag
    54481 aggcgcgctg cggtgtgcgt tgtgccaccc gcgatcatct gccaatggta ggcaatgttc
    54541 ccgattatga ggcaacactc gtggaatatg cgtcgttggc ggagcaaaaa gatgaggcgg
    54601 taagcgcgcc ggtttttgac gatctcttta tgtttgcggc tttaggttct cgcggtttgt
    54661 gttctgcccc gctgtgtgcc gagattctgg cggcgcagat gagcgacgaa ccgattccga
    54721 tggatgccag tacgctggcg gcgttaaacc cgaatcggtt atgggtgcgg aaattgttga
    54781 agggtaaagc ggttaaggcg gggtaatctg ctctcgcagt gtttgccgga tgcggcgtga
    54841 acgccttatc caacctacgt gtgacgtgta ggcctgataa gacgcgataa gcgtcgcatc
    54901 aggcatggtg ctccagacgc cgcaacttac agctgtgacg cctgctgaaa caggttttcc
    54961 cacatatcgg tcaccagcgc ctggtcacgc ggcgacaatt caccggcacc aatggctttt
    55021 tccagactct ggctaacggt cgtatgcacc gcctgagcgg agtggtcatc accactttcc
    55081 agttctgcga tggctaatgt caggtggcca cgcaaatacc cactggcaaa caactcatca
    55141 tcactggcat ggtcaaccat gccgtcgatt aatgccagaa tgcgtgattc aaactccgcg
    55201 atcatcttct ttcctcattg taaaacgtgg cgctggcttc agttgagcgc ttccggccac
    55261 gggaactgtt ccgccgtaag ttccggcgtg tgataataat tttgtagtgc tttaatgaag
    55321 cgtgccgggc gttccggaat gccatttgtc agataatcca tcacctgcgc atgtacccgc
    55381 cgttgaaaca ccacacgatc cggttcgaaa tccccttcca gattgtcgca actgacgtta
    55441 aacggatatc cagccgccac gcagaataac cagtcgaacg cctgcggctt cacttcaaca
    55501 tcttcaaact ggctttgcgt ctgggcatcg cgtccgtccg ggcaatacca gtagccgaaa
    55561 tcaaccagct cacggcgcgc ttttccggca atacaccagt gcgaaatctc atgaattgcg
    55621 ctggcataaa agccatgagc aaagacgatt cggttatacg gtacttccgc atcagcagga
    55681 agatagatcg gttcgtcgtc gcctttaatc agacgggtat taaattcatc ggcaaagcag
    55741 ctattaaaaa tttcaatcaa ctgctcgtag tggtgtgtac tgttcattag ttcatcccca
    55801 accagtggag gatctcctgt ccgtggctat cgtaaagtaa tttggcactc atcaccgccg
    55861 agacgataac aatcatcggg cggatcagct tttgtccttt gctcaacacc aaccgtgaac
    55921 ccatgcgcgc gccgaggaac tgccccacca gcatgacaaa gcccgtcgcc caaatcactt
    55981 tgccgccgag aataaacagc agcagaccgc caatgtttga cgttgcgttg agtaatttgg
    56041 catgagccgt ggctttggcg aggttgaatc cgcacagcat aacgaaggcc agcgcgtaaa
    56101 acgatccggc agccgggcca aagaatccgt cataaaaacc gacgcagcca ccggcaatta
    56161 acgcgaaggg taaaccgtac atccggcgct ggcgatcttc ttcacccagc tttggcatca
    56221 gcaagaaata gaggccgata caaatcacca gaatgggcaa aatctgccgt aagacatcag
    56281 cctgaacgta ctgtaccaac agtgcgccgc tcattgagcc gacaaaggtc atggcaatat
    56341 tgagtttctg atcgcttaaa ctaaccactt tgcggcgaat aaagtagata gtggcggaaa
    56401 tagagccgcc gcaggcttgc agtttattgg ttgccagcgc gttagcggga gacattcccg
    56461 cagccatcaa tgccggaatg gtgagtaacc caccgccacc ggcaatcgag tcgataaatc
    56521 ccgccagcat ggcgacaaaa aagagaactc ccagcaacag cggagaaacc ataaacaggc
    56581 tattaaacgt ttccattaga tcacgtgctc atccagtagc gcctggcagg aaggcggcaa
    56641 cggaggcggt gtcttcttct caggctttgt tgttcccggt tttggaggtt caaaccagct
    56701 ttgcagttct gccccgcaac catcgcctga tggcggtaaa ggttgatctt cacactccag
    56761 actatcggca gggcaacgta atcgtacatg catatgcgcg cgatgctgga accagggtcg
    56821 cactttgcgc aaccagtcgc gatcggtgcc cgcatcaagg caaagttgtt gtttaatcgc
    56881 cggattaaca aaaatgcgcg tgacgtcttt gtcctgggcg gcgagtttga tcaagctgaa
    56941 aatttctggc ttccacagcg tggagacaac gtgtttaccg tcgcgggaaa ctaagtccag
    57001 tgcttgcggg cgcaagagct gcgcggaggt ccagcgagtt ttcggcagtt gcagaaagat
    57061 atcgacatcc agtccggtct ggtggctggc atgaccgccg ttgaaacgcc caccagcggg
    57121 catccccata tcgccaatca gcaccgtacc catgcccaga ttgctcacct ggctactcag
    57181 acgctggata aacatcacca gatccgggtg accgaaatag cgacgctgat cggtacgcat
    57241 gacctgataa tgttcggact gtatcggcaa cgtgtcagcg ccgacaatac agccattaga
    57301 aaaactgcct atcgattgtg cgctacccgg cacaggttgg gttatttttt gccacggcgt
    57361 cgctgccagg ctagcactac tggcaagcag agccagcagc gcaatcgcgg ttttgttcat
    57421 tttttaccag cgtggaatat cagtcttcac atcggcattt tgcgcccgct gccgtaacag
    57481 gtgatccatt aaaacgatcg ccagcatcgc ttcagcgatc ggcactgcac ggatcccgac
    57541 acagggatcg tgacggcctt tggtgatcat ctcaacttct tcgccaaagc ggttaatagt
    57601 gcgccccggc acggtaatgc tggaggtcgg tttcagcgcc atatgagcaa tgatttgctg
    57661 cccgctgctg ataccgccga aaatgccgcc cgcatggttg ctctggaaac cgtctttggt
    57721 gatttcgtcg cggttctggc tgccgcgcag cgccactacg tcaaaaccgt cgccaatttc
    57781 cacgcctttc accgcgttga tgctcatcag cgcatgggcg atgtcggcat ccaggcggtc
    57841 aaagaccggc tcgccaagtc cggcggggac accactggca acaacggtga ctttcgcgcc
    57901 gatggagtcg ccctcttttt tcagcgcgcg catcagttca tctaacgcgt cgattttgtc
    57961 ggggtccggg cagaagaacg gattttgttc aaccagcgac cagtctttga tttccagcgg
    58021 aatgtcgccc atctgggtca ggcagccgcg gatttcaata ccaaatttct cggcgagata
    58081 ttttttggca atcgcccctg ccgccacgcg catggcggtt tcgcgggcgg aagaacgtcc
    58141 accgccgcga taatcgcgca gaccgtattt ttgttcgtag gtgtaatcag catggcctgg
    58201 acggaagacg tctttaattg cgctgtaatc ctgagaacgc tggtcggtat tttcgatcaa
    58261 caagccaatg ctggtgccag tagtaacgcc ttcaaaaaca ccggagagta ttttgacctg
    58321 atccggctcg cggcgctggg tggtatagcg cgatgtccca gggcgacgac gatcaaggtc
    58381 atgttgcagg tccgcttccg tcagcggaat gcctggcgga acaccatcga cgatgcagcc
    58441 gagcgccagc ccgtgcgatt cgccgaaggt ggttacgcga aagagttgtc caattgtgtt
    58501 tccagccatc acggctccgt tattgttgtg tttgcgtgtt tacttaatct ttataaatcg
    58561 cgaaatgttc tcgtgcggca ataagctgct ctttagtgag cataaacaca ccatcgccgc
    58621 cgttatcaaa ctccagccag gtgaacggaa catcgggata ttgttccata agatgtacca
    58681 tgctgttgcc cacttcacaa atcaacacgc catcatcagc aaggtaatct gccgcgttac
    58741 cgagaatgcg acgcgtcagt ttcaggccgt cggtgccaga tgccaggccc agttccggct
    58801 cgtgacggta ttcgtttggc aggtcggaca tatcttccgc gtcgacatac ggcgggttag
    58861 tgacaatcag atcgtattgc actttcggca aatcacggaa cagatcggaa cgaatcggaa
    58921 tgacgttgtg gatcagaccg tgttcttcga tgttctgttc ggcaaccgcc agcgcgtcgg
    58981 gagagatatc taccgcgtcg acttccgctt ccgggaaggc ataagcacag gcaatggcga
    59041 tgcagccgct accggtacac atatctaaaa tatgctgcgg ttgcttgctg atgagtccgg
    59101 caaatttatt gttgatcagt tcaccaatcg gcgagcgcgg caccagcacg cgttcatcga
    59161 cgtaaaattc atgaccgcag aaccaggctt tgttggtcag gtaagccacc ggaatgcgct
    59221 cgttgacgcg gcggatcacg cgttcaacaa tacggtgttt ttcgctggag gtcagacgcg
    59281 cggtgcgcat atcttccggg atatccagcg gcaggtagag cgatggtaac accagctgta
    59341 cggcttcatc ccacgggtta tcggtgccgt gaccgtacca gatatttgcc gcgctgaagc
    59401 ggcttaccga ccagcgcaat atgtcctgaa tggtttgcag ctcatttact gcttcatcaa
    59461 cgaaaatttt atccacgtat tcctccaggg catgatcgca ataatttcgg cggctagttt
    59521 gccatgaaga tgacgataaa tcagcattca cgcgcggtga gtgaggaaaa atacgtttaa
    59581 aacgatcgat tgtgctacga gtcgggtaaa ctgtaggaaa attagaaata gagacagata
    59641 aatgaaaaag aaaacaacac ttagcgagga ggaccaggct ctgtttcgcc agttgatggc
    59701 ggggactcgc aagattaagc aggacacgat tgtccaccga ccgcagcgta aaaaaatcag
    59761 cgaagtgccg gtgaaacgct tgatccagga gcaggctgat gccagccatt atttctccga
    59821 tgagtttcag ccgttattaa ataccgaagg tccggtgaaa tatgttcgcc cggatgtcag
    59881 ccattttgag gcgaagaaac tgcgccgcgg cgattattca ccggaattgt ttttggattt
    59941 acacggtctg acgcagctgc aggccaagca ggaactgggc gcgttgattg ccgcctgtcg
    60001 ccgcgaacat gtgttttgtg cctgcgtgat gcatgggcac gggaagcata ttttgaagca
    60061 acaaacaccg ctgtggctgg cacaacatcc gcatgtgatg gcatttcacc aggcaccgaa
    60121 agagtacggc ggtgatgcgg ctttgttggt gttgattgaa gtggaagagt ggttgccgcc
    60181 ggagttgccc tgaatgctgg cagcaaatgc cggagcagcg taaacgcctt atccggccta
    60241 caaatatgtg ataattcaat aaattgcaat tcacttcgta ggcctgataa gacgcatcag
    60301 agcaatacta cgaacttcag ttcattcaat acatactgaa aacgacacgt cgaatcaatc
    60361 ggttgcccac tactggtatt cacattcacc acaatatcgt gtagtacttt atccgttacc
    60421 ggatgacgag ttggcgtaac cgttaccgtt gatttatcac aaccgtaaga ggtaaaggtt
    60481 aacaattctg ataccgcgcg gcagtatgct cctctgccat cattatcaga aaaattactg
    60541 ccatcacaga gtctgccgcc ttccatcagg taggtcgaaa gcgtggtggt cactgttgac
    60601 cccgtggact gaattcgtaa cgccttatta tggctgacat aattttcact ccaggagata
    60661 tcgaatgaag cgaccggtat cgttgggcat attgtgttat gcactgtagg ggccgtatag
    60721 cttccaaatc cgggtaaatc tgtataggaa taaaaggatg cgatccaagg gcaaccataa
    60781 tcatcagatg caacggtaat tttaaacgtc atatatgcac aacgaaaaat tttcgtatct
    60841 atgccagggc aatttgagtt attaattgta tcaaagtcag tcagtaccaa agttggagct
    60901 gaacccgacc cagaccagga aataacccgc aacgtatcgc ctttatacgc aaaagaatca
    60961 tatttcccat atgcaacctc ctttggcgtt atcaccgtaa cattgaaaac actgctcgct
    61021 acagcaccgg ttaccttgtc catggcataa taaactctca cactgtcaaa actcgcactc
    61081 ataagtttat ctccagcata actcttagat gcgccgacaa cactcagaac gagcagcatt
    61141 aggctcgcga aaattatggt ccattttttc atttcagaaa tactcaaagg ttgccgtcac
    61201 tgtggcacta aagtctccgg acgttacatc tcgcccgctc tttgcctgta accaggtatt
    61261 aaaatttagg ctgttatttc cctgattcag cgggagcgtg acgcctgttg tattgttgat
    61321 tggcaccgat gtgcctgccg cagtttcaat accaatccca gcgccggatg ctgatgacga
    61381 ggagtcaaag gccagaaagc cgggtagagc actatcttct gttcctgtca acgttacttt
    61441 gacattaaac aatgtagaac tatgacaatc tttcaattga aacaccaatg gcactcgctc
    61501 tgactgccca aggttcatta aatcactggc ggcaatggca ggaaaatgta cttctgccaa
    61561 ctggctacca ttaatcactg gcgtacacga tttactgatt aaattgccgg taaagtgcaa
    61621 attatctcct gctattgtgg ttccactgcc cagcaacatc aggctactaa taaaaaacaa
    61681 cgtacgcttt ttcttcattg gtaatctacc tgtaacgtgg cccaggcctc aaagtccgcc
    61741 tctggcagaa tgacaccact cttttttact ggcacagccg ttaacaccgg taaatcggtt
    61801 tcattcacca caagtggcgt attgatcgta aaactctgcc ctgcctgttt gagctgaatg
    61861 cctaaaccag tgatattgct gcttactgca gcattatcaa aatcgcttgg tgtgccactc
    61921 cagctcaagg tcatttccat taatgggtcg cgaaccgccg tatcgcaggt aatggaatac
    61981 ggcacggttt gctcataatt gctgccatta atgtcatcaa taagcacatc ctgaaaagtt
    62041 atttcaatgg tttgagcgtt gctaatcgtg cagttaggcg gctgcactaa ataaccgtga
    62101 aaagagacgt ttgagagtgc catcgagcaa aagccccaca gcaaaataag cgagatccgt
    62161 ttcattggta atccaccaca attgtggcac tggcgttaaa ttcagctgcc gctaacgttg
    62221 taccactctg tttcaccggc acagcttcca gaacgggaac tcccgaggag aaattaaatg
    62281 gcaaccatga gccagaggtt aagtcgagta ttgtgtgatc actacttttc tgaacccgaa
    62341 taccaaaccc aggaataccc gtgctgataa ctgtttcacc gttaatcacg accgttgtcg
    62401 cctgcatctg tagacgcagg tcatccattg ccagattgtt acaaacaagc gagtagtcaa
    62461 tgggacgttt ataatcaacc ctattgatct tattaatgaa tacattacca aactcaactg
    62521 ccgcaccatt taccgtacaa ggaggagggg catcgacaag cacccgtaaa ttcatactta
    62581 ctgacttact cgctgcaact gcaaagccgc taccaacaca acatatcagt aacccagtta
    62641 ccgcccagac agaacaagat cctttcatac tggctctccc ggcctcagcc tttgcggctc
    62701 tgctcttcat cgacctagca actgttaccg ttgcaagtga aaaagagcgg catccgagcg
    62761 ccatagtcgt tgacataggt cagtaccggc acgctaccca tcttcacatt cagcggtgca
    62821 gaggttttcg gcgacatcac tattggcgag aaaccggctg ccggattgcc atttttctgt
    62881 gtgctggcat tactgaaaat gacgtaatac gacgtcgggt tattgacggt aaattcctga
    62941 ccgttgcggg ttaaagtaat cttatattgc taggggtttt tcatgctgac gttctccagc
    63001 gccttcggtc gccagaacaa cttaatacgc gtttgtagtg ctatttgcag cgtgttggct
    63061 ttgtttgatt ttggcgggat ctcacgcacg ttgaagtaga acaggctttc acgatcggca
    63121 ggcaatttgt tgatatccgg cattccctgc acttttacct gcccgttcat cattgaatcg
    63181 atgcgttgca ccggtggcag aacagttaac ggtgaactga tcttgttacc cttttcatct
    63241 tcgatccagc tttgggccag ataaggcagt ttcggatcgt tattgcgcaa cgtcacgctg
    63301 attgatttgt cgctttcatt aaatatcaaa cgggtacgat ctggcgttac agaagcctgg
    63361 gcagacaaac tggcagcgct aagcagtaac gctagcgtcg tagcacccaa tttggcagag
    63421 caaagtaaat cagacatgga tcattcctta atcacgttat tggttagctg aaacagaaac
    63481 agggggattg gtgggtgtca cctgttgcgt ctgctcctga atgacaggct ttatgtcatc
    63541 aggaacaacc ggcgcgacgt tgcctttatg ctggcatggc agtaacaagc cgttaaacag
    63601 atcggcaggc agcgggtccg gcaggttgat atcgcaatgc gcaacaccac tccagaacac
    63661 actcatgtgt tcgccaggtt tcactccagc cagataaaca ttgccgtcat catcgacaag
    63721 gcccactgtc tgctcgttat cattttttac ttctgcgcca aacggaggat ggctgccgtc
    63781 ttgtaaacgc agcaccgcca tcgctttttg accactgatg acggcaaatt tgcggtagcc
    63841 aatcgcccct tcagttagcg tggcttgtac caccgactgg gttgcttcgg cgttttccgg
    63901 cagtttgttg aggtcgatat acgcctgatt gcggtaatag ttattgacgt cagaaacgac
    63961 ggctttacca aacatattgg tataaacagc agcaccgtta ccttctaccg gaacatcggc
    64021 aacgccatcg gcatcaatca acaagcgtgt cccgcccata ttctgggtac ggtgaagtgc
    64081 gccaccgtgg gcagtaagcg ttgcgccgcc ctgtaacgac aagcccgcag aggtgtattg
    64141 cccttcatgg tagttcgcac tgaggtcaac ctgcgccagc gaaccatcat ggctgtaata
    64201 gccgtcaacg ctggtgtgtt tgtcactggt gccgacgttc aactgatagt gagtcgcgtc
    64261 atcgacacgg ctgaaataac cgacctgact gctgtccgtc ccactgccat agtaccgtta
    64321 tagctaacgg tactgttgtc gccccacggc atactgagcg aaatgtacat gcctttgtcg
    64381 gcctggttgt catactcgta acggtagcca gtcatcgaga tgctgacatt acgaatactg
    64441 cccatattga aatagtgcga gagcatgatg ttgtagtttg tctgctcctc gcgatcccag
    64501 taggtatggc gggtatagtt gagataaacc gaaacacccg catcgcggaa gttctggtta
    64561 taagtggcgg tgtacatctc tttgtcgttg cccgtgcgta ccattccgct gtcactggca
    64621 tccagatact cgctcatggt cataaagttc tcttccgaga agcgatatcc agcaaaagta
    64681 acgcggctgt tgagctggtc aaagtctttg gaataactca cacggaagga gttaccgtcc
    64741 agcgaacctt tgccataagc ggtgtcttta tccagtttgg tgtgcgagtg agtaacatca
    64801 aacgcgaccg cgccgaatgt agacaaatcg cgaccgacac caagcgccgc agactgatag
    64861 ttttcatctc ccagtgcgcc gccatataac gaccagccgt tagcgatccc ccaggaagct
    64921 tcagcaccag aaaaaaattc accctcgaca tggtgtcccc actcttgcgg acggcccatc
    64981 atgatcttat agcgaacctg acctggacga gtgaggtatg gcatcgaggc ggtgctgatg
    65041 tcatattcct gcacctggcc gttctgttct tcaatgcgaa tatgcaacgt accggagaca
    65101 gaatcaccaa gatcctgaat acgaaacggt ccagccggaa cctgcgtttc gtaaatcaca
    65161 cgccccatct ggctgacggt cacttttgct gtggtgtgtg ccacgccgga aatgtctggc
    65221 gcgtagccgc gcagattggg aggcaacatt tgatcgtcag tactgacact gccaccaaca
    65281 tagttaaaac catcaaaaat atcggatctg aggtaatcct cgcccagcgc cagtttggct
    65341 tttaatgatg gtaacgcccg cctggcatag tagcgactcc actcccattt ttttgagttt
    65401 catcgccgct gaattcatcg tcatcattac tgcgagtatg ttgatagtta gtctgccagt
    65461 cagcacgcat acgccacggc cctaggttaa ccccaaccgt cccgttgccg ctgatctcgt
    65521 tactatcatc accgccattt tcttcgtgcc gggtttgtgc gttgatgctg tagtccgcga
    65581 cgatcccgga gatgccgtca tcccaacgtg aaggcggatc cctgtcggga taagtatatt
    65641 cgaggtaagc ctgtggcagt gaaatgacta atgcggactg gcttaaatca gccttaattt
    65701 ccatgccttc cagttgaccg gatttcaggc atttagcatc gtggctccat tgcagatttt
    65761 tcgccacgtc ttcttttaaa ccaaactgcg ctaccagttc cggtgtcaga caagcatagc
    65821 ttttgctcgc gtcatcttca ccagcatacc agtaaatatc gtactcttcc gccaatggct
    65881 gtttatttag ttgaacctgt aaattgtatt ttccgggctc aacatatccc tggctggaaa
    65941 aacgcttcat atcaattttt gtgtcgcctt ttaattccag aaaacgggaa tcaaactgaa
    66001 tgtcgtcttc agcccagaca ctgctataac tccctgacat tgccagcgca atgcaccacg
    66061 gaagaatccg taatcgaaaa agagaatggt caggcataca cgtttctatc cgtttttatt
    66121 cttgttcggt caaaatacat ttaaatcccc catagcagtg tcgctatggg ggtatgcctt
    66181 acaggtaagt aatctggaag gtggtatttg cttcaaaatt acccagatct ggcgcatcag
    66241 ctgcgccgac cagccaggcg ttaaagttca gtgtctgctt cgctttgcct ttgttggtag
    66301 aagtgtcttt cactattttc tggtcaatac ccatgccgct tttgtaagag gtacccagag
    66361 agtcaccgat cgccaggcta acattgttaa atgccgcacc ggtatcggta ttgaaaatgg
    66421 tgtaataatt gccgctattt gcagaagaaa cggtaccagt aaaggtagtg gtcattgttt
    66481 cctgagtatc aaatacgcaa tcttgcagac gaatctggaa acttttcggc gtggtggtgc
    66541 cgttattttt cagtttttcg gcaccgatat cacccatatc aacttccagt ttgtgatcgt
    66601 ccggaacgat agaacacact gagtcttcaa tggtgccgta aaaacgtgca gtaccgttgt
    66661 tacccgcagc gattgttgcc gtagaggtga acacgcccat caccattgcc gcagcaatag
    66721 ctgttttaac aaacttactc attatttgtc cttaaactat aaataattaa aaatataaac
    66781 attgcaatac attgattcag tcaatagcca atgtttatac ccagccattg aatacggcat
    66841 gcgacccaaa aagaacaggt cgtagaaaat tgtattcatt ggtgtggggc cgcatagtga
    66901 tgtttccaca cccctgatcc aatttaattt tactaaagag taaatgtaga tttaattaat
    66961 atattgacag aagaaaggtt atcttaagga
//
LOCUS       DAZPCS010000004        65716 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZPCS010000004 DAZPCS010000000
VERSION     DAZPCS010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65716
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.004"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(128..607)
                     /gene="folK"
                     /locus_tag="ACHUQ7_000212"
     CDS             complement(128..607)
                     /gene="folK"
                     /locus_tag="ACHUQ7_000212"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFR1133798.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(604..2001)
                     /gene="pcnB"
                     /locus_tag="ACHUQ7_000213"
     CDS             complement(604..2001)
                     /gene="pcnB"
                     /locus_tag="ACHUQ7_000213"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFR1133799.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTT"
     gene            complement(2061..2957)
                     /gene="gluQRS"
                     /locus_tag="ACHUQ7_000214"
     CDS             complement(2061..2957)
                     /gene="gluQRS"
                     /locus_tag="ACHUQ7_000214"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFR1133800.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQIPLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWMLTAVPESAIVNSTFSNASC"
     gene            complement(3024..3479)
                     /gene="dksA"
                     /locus_tag="ACHUQ7_000215"
     CDS             complement(3024..3479)
                     /gene="dksA"
                     /locus_tag="ACHUQ7_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFR1133801.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3657..4361)
                     /gene="sfsA"
                     /locus_tag="ACHUQ7_000216"
     CDS             complement(3657..4361)
                     /gene="sfsA"
                     /locus_tag="ACHUQ7_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFR1133802.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4376..4906)
                     /gene="thpR"
                     /locus_tag="ACHUQ7_000217"
     CDS             complement(4376..4906)
                     /gene="thpR"
                     /locus_tag="ACHUQ7_000217"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFR1133803.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4980..7409
                     /gene="hrpB"
                     /locus_tag="ACHUQ7_000218"
     CDS             4980..7409
                     /gene="hrpB"
                     /locus_tag="ACHUQ7_000218"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFR1133804.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGLNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGVLSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPKILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANSMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7605..10139
                     /gene="mrcB"
                     /locus_tag="ACHUQ7_000219"
     CDS             7605..10139
                     /gene="mrcB"
                     /locus_tag="ACHUQ7_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFR1133805.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVAITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10359..12602
                     /gene="fhuA"
                     /locus_tag="ACHUQ7_000220"
     CDS             10359..12602
                     /gene="fhuA"
                     /locus_tag="ACHUQ7_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFR1133806.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLFNLYNPVNPDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12653..13450
                     /gene="fhuC"
                     /locus_tag="ACHUQ7_000221"
     CDS             12653..13450
                     /gene="fhuC"
                     /locus_tag="ACHUQ7_000221"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFR1133807.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13450..14340
                     /gene="fhuD"
                     /locus_tag="ACHUQ7_000222"
     CDS             13450..14340
                     /gene="fhuD"
                     /locus_tag="ACHUQ7_000222"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFR1133808.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14337..16319
                     /gene="fhuB"
                     /locus_tag="ACHUQ7_000223"
     CDS             14337..16319
                     /gene="fhuB"
                     /locus_tag="ACHUQ7_000223"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFR1133809.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLIFAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16354..17634)
                     /gene="hemL"
                     /locus_tag="ACHUQ7_000224"
     CDS             complement(16354..17634)
                     /gene="hemL"
                     /locus_tag="ACHUQ7_000224"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFR1133810.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFAVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17800..17847
                     /gene="yadX"
                     /locus_tag="ACHUQ7_000225"
     CDS             17800..17847
                     /gene="yadX"
                     /locus_tag="ACHUQ7_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFR1133811.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            17859..19280
                     /gene="clcA"
                     /locus_tag="ACHUQ7_000226"
     CDS             17859..19280
                     /gene="clcA"
                     /locus_tag="ACHUQ7_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFR1133812.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMGLGREGPTVQIGGNISR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19304..19369
                     /gene="yadW"
                     /locus_tag="ACHUQ7_000227"
     CDS             19304..19369
                     /gene="yadW"
                     /locus_tag="ACHUQ7_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFR1133813.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            19362..19706
                     /gene="erpA"
                     /locus_tag="ACHUQ7_000228"
     CDS             19362..19706
                     /gene="erpA"
                     /locus_tag="ACHUQ7_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFR1133814.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19753..20376)
                     /locus_tag="ACHUQ7_000229"
     CDS             complement(19753..20376)
                     /locus_tag="ACHUQ7_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFR1133815.1"
                     /translation="MLVYWLDIVGTAVFAIYGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GISVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLVREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20414..21214)
                     /gene="btuF"
                     /locus_tag="ACHUQ7_000230"
     CDS             complement(20414..21214)
                     /gene="btuF"
                     /locus_tag="ACHUQ7_000230"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFR1133816.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21207..21905)
                     /gene="mtnN"
                     /locus_tag="ACHUQ7_000231"
     CDS             complement(21207..21905)
                     /gene="mtnN"
                     /locus_tag="ACHUQ7_000231"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFR1133817.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21989..23506
                     /gene="dgt"
                     /locus_tag="ACHUQ7_000232"
     CDS             21989..23506
                     /gene="dgt"
                     /locus_tag="ACHUQ7_000232"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFR1133818.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23636..25060
                     /gene="degP"
                     /locus_tag="ACHUQ7_000233"
     CDS             23636..25060
                     /gene="degP"
                     /locus_tag="ACHUQ7_000233"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFR1133819.1"
                     /translation="MKKNTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKKLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25215..26371
                     /gene="cdaR"
                     /locus_tag="ACHUQ7_000234"
                     /pseudo
     CDS             25215..26371
                     /gene="cdaR"
                     /locus_tag="ACHUQ7_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(26460..26846)
                     /locus_tag="ACHUQ7_000235"
     CDS             complement(26460..26846)
                     /locus_tag="ACHUQ7_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFR1133820.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27160..27984)
                     /gene="dapD"
                     /locus_tag="ACHUQ7_000236"
     CDS             complement(27160..27984)
                     /gene="dapD"
                     /locus_tag="ACHUQ7_000236"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFR1133821.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28015..30687)
                     /gene="glnD"
                     /locus_tag="ACHUQ7_000237"
     CDS             complement(28015..30687)
                     /gene="glnD"
                     /locus_tag="ACHUQ7_000237"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFR1133822.1"
                     /translation="MNTLPEQYANTALSTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30749..31543)
                     /gene="map"
                     /locus_tag="ACHUQ7_000238"
     CDS             complement(30749..31543)
                     /gene="map"
                     /locus_tag="ACHUQ7_000238"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFR1133823.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31911..32636
                     /gene="rpsB"
                     /locus_tag="ACHUQ7_000239"
     CDS             31911..32636
                     /gene="rpsB"
                     /locus_tag="ACHUQ7_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFR1133824.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32894..33745
                     /gene="tsf"
                     /locus_tag="ACHUQ7_000240"
     CDS             32894..33745
                     /gene="tsf"
                     /locus_tag="ACHUQ7_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFR1133825.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33892..34617
                     /gene="pyrH"
                     /locus_tag="ACHUQ7_000241"
     CDS             33892..34617
                     /gene="pyrH"
                     /locus_tag="ACHUQ7_000241"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFR1133826.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34909..35466
                     /gene="frr"
                     /locus_tag="ACHUQ7_000242"
     CDS             34909..35466
                     /gene="frr"
                     /locus_tag="ACHUQ7_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFR1133827.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35558..36754
                     /gene="ispC"
                     /locus_tag="ACHUQ7_000243"
     CDS             35558..36754
                     /gene="ispC"
                     /locus_tag="ACHUQ7_000243"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFR1133828.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASEVARKE
                     VMRLAS"
     gene            36943..37701
                     /gene="ispU"
                     /locus_tag="ACHUQ7_000244"
                     /gene_synonym="uppS"
     CDS             36943..37701
                     /gene="ispU"
                     /locus_tag="ACHUQ7_000244"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFR1133829.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37714..38571
                     /gene="cdsA"
                     /locus_tag="ACHUQ7_000245"
     CDS             37714..38571
                     /gene="cdsA"
                     /locus_tag="ACHUQ7_000245"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFR1133830.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38583..39935
                     /gene="rseP"
                     /locus_tag="ACHUQ7_000246"
     CDS             38583..39935
                     /gene="rseP"
                     /locus_tag="ACHUQ7_000246"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFR1133831.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFDSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39965..42397
                     /gene="bamA"
                     /locus_tag="ACHUQ7_000247"
     CDS             39965..42397
                     /gene="bamA"
                     /locus_tag="ACHUQ7_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFR1133832.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42519..43004
                     /gene="skp"
                     /locus_tag="ACHUQ7_000248"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42519..43004
                     /gene="skp"
                     /locus_tag="ACHUQ7_000248"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFR1133833.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43008..44033
                     /gene="lpxD"
                     /locus_tag="ACHUQ7_000249"
     CDS             43008..44033
                     /gene="lpxD"
                     /locus_tag="ACHUQ7_000249"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFR1133834.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44138..44593
                     /gene="fabZ"
                     /locus_tag="ACHUQ7_000250"
     CDS             44138..44593
                     /gene="fabZ"
                     /locus_tag="ACHUQ7_000250"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFR1133835.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44597..45385
                     /gene="lpxA"
                     /locus_tag="ACHUQ7_000251"
     CDS             44597..45385
                     /gene="lpxA"
                     /locus_tag="ACHUQ7_000251"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFR1133836.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45385..46533
                     /gene="lpxB"
                     /locus_tag="ACHUQ7_000252"
     CDS             45385..46533
                     /gene="lpxB"
                     /locus_tag="ACHUQ7_000252"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFR1133837.1"
                     /translation="MTDQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46530..47126
                     /gene="rnhB"
                     /locus_tag="ACHUQ7_000253"
     CDS             46530..47126
                     /gene="rnhB"
                     /locus_tag="ACHUQ7_000253"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFR1133838.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            47163..50645
                     /gene="dnaE"
                     /locus_tag="ACHUQ7_000254"
     CDS             47163..50645
                     /gene="dnaE"
                     /locus_tag="ACHUQ7_000254"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFR1133839.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50658..51617
                     /gene="accA"
                     /locus_tag="ACHUQ7_000255"
     CDS             50658..51617
                     /gene="accA"
                     /locus_tag="ACHUQ7_000255"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFR1133840.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51716..53857
                     /gene="ldcC"
                     /locus_tag="ACHUQ7_000256"
     CDS             51716..53857
                     /gene="ldcC"
                     /locus_tag="ACHUQ7_000256"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFR1133841.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GISTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVKTAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECRPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53914..54303
                     /locus_tag="ACHUQ7_000257"
     CDS             53914..54303
                     /locus_tag="ACHUQ7_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR1133842.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54368..55666
                     /gene="tilS"
                     /locus_tag="ACHUQ7_000258"
     CDS             54368..55666
                     /gene="tilS"
                     /locus_tag="ACHUQ7_000258"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFR1133843.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSATLCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(55715..55975)
                     /gene="rof"
                     /locus_tag="ACHUQ7_000259"
     CDS             complement(55715..55975)
                     /gene="rof"
                     /locus_tag="ACHUQ7_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFR1133844.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55962..56162)
                     /locus_tag="ACHUQ7_000260"
     CDS             complement(55962..56162)
                     /locus_tag="ACHUQ7_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFR1133845.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56328..56873
                     /locus_tag="ACHUQ7_000261"
     CDS             56328..56873
                     /locus_tag="ACHUQ7_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFR1133846.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56870..57292
                     /gene="arfB"
                     /locus_tag="ACHUQ7_000262"
     CDS             56870..57292
                     /gene="arfB"
                     /locus_tag="ACHUQ7_000262"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HFR1133847.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57306..58016
                     /gene="nlpE"
                     /locus_tag="ACHUQ7_000263"
                     /gene_synonym="cutF"
     CDS             57306..58016
                     /gene="nlpE"
                     /locus_tag="ACHUQ7_000263"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFR1133848.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(58171..58995)
                     /locus_tag="ACHUQ7_000264"
     CDS             complement(58171..58995)
                     /locus_tag="ACHUQ7_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HFR1133849.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSDLAKAQLSSVCEGDKKSWFCSEFVTDAFAKS
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(59048..60766)
                     /gene="proS"
                     /locus_tag="ACHUQ7_000265"
     CDS             complement(59048..60766)
                     /gene="proS"
                     /locus_tag="ACHUQ7_000265"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFR1133850.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60877..61584)
                     /gene="tsaA"
                     /locus_tag="ACHUQ7_000266"
     CDS             complement(60877..61584)
                     /gene="tsaA"
                     /locus_tag="ACHUQ7_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFR1133851.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKGSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61581..61985)
                     /gene="rcsF"
                     /locus_tag="ACHUQ7_000267"
     CDS             complement(61581..61985)
                     /gene="rcsF"
                     /locus_tag="ACHUQ7_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFR1133852.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62103..62918)
                     /gene="metQ"
                     /locus_tag="ACHUQ7_000268"
     CDS             complement(62103..62918)
                     /gene="metQ"
                     /locus_tag="ACHUQ7_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFR1133853.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62958..63611)
                     /locus_tag="ACHUQ7_000269"
     CDS             complement(62958..63611)
                     /locus_tag="ACHUQ7_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFR1133854.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVCKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63604..64635)
                     /gene="metN"
                     /locus_tag="ACHUQ7_000270"
     CDS             complement(63604..64635)
                     /gene="metN"
                     /locus_tag="ACHUQ7_000270"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFR1133855.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQTEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64823..65398
                     /gene="gmhB"
                     /locus_tag="ACHUQ7_000271"
     CDS             64823..65398
                     /gene="gmhB"
                     /locus_tag="ACHUQ7_000271"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFR1133856.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT        15544 a        16281 c        18103 g        15788 t
ORIGIN      
        1 tgggattgat atttatctgc gggaatttat attatgtttc tgcaaaaatg tttaaactaa
       61 aatttttatt aaggaaagag aggatgtatg attttcagaa aatatttata ttcgtaatat
      121 aaataaatta ccatttgctt aatttgtcaa atgctcttgt atgtaagact tcacgcaacg
      181 tctccccatc aggaaacgcc agctccggtg caatctcaaa cagcggccac agcataaatc
      241 cacgattctt catatcgtag tgcggaacgg tcaggcgttc ggtatttatc acttcattac
      301 caaacagcat gatatcgaga tccagcgtgc gtggtcccca gcgttcagct ttacgaacac
      361 gaccctgttg cagttctatg cgctgtgtat ggttgagtaa ctcttccggg gcgggcgaag
      421 tttcaagcgc cacggcggcg ttgaggtaat cgggttgatc ttgcggcccc agcggtgggg
      481 tgcggtaaaa cgaagaaacg gcaagaatgc ggctttcagg gatatcgcct aatgctttca
      541 gggcagcatt gacctgctcc agcggagagg ccagattgct gcctatggca atatacgcca
      601 ctgtcatgtg gtaccctcac gacgcggtgc gcgtttgcgt ggacgacgag tacgacgacg
      661 cggtgacggt tcttcatcca gctcgttaag catccctttt tgatctggtg gtgcggaaac
      721 ctggaactca ccccaccatt tcaccagacg ctgcagttca gcattacgtt caacttcagc
      781 tcgcaaggcc aacaggtcat aagccgcacg gaacttagga tgctccagca gtttccatgc
      841 gcgtttaccc tgacgacggg acatacgcaa ctgcaactgc cagatatcgc gggttaacgt
      901 cgtcagacgt ttcgggattg ccagtgaacg gcaggcttcg tccagcacgt cgttcatcgc
      961 cagcgcgaaa gcgtcgtgat aggtcaggcc gctttcctgg gcgatcttct gtgccgtctc
     1021 cagcagtggg taccagaaca tggcggcaaa caagaacgcc gggttcacgc gcatatcgtt
     1081 atggatacgc gtatcggtat tcttcagcac ctgttcaatg atccgctcca tcgggctgtc
     1141 gccattttcc gtgaagtagc gggtaatggt cgggaacagc ggctggaaca gatgatattc
     1201 acacaacagc ttataggttt cgtaaccgta gcccgcttgt agcagtttaa gcgattcttc
     1261 aaacaggcgt gccggtggga tatcgttcag cagggtagcg aggcgaggga tcggttctgc
     1321 ggtttccggg ctgatgcgca tacccaattt ggcggcaaaa cgtaccgcgc gcagcatacg
     1381 taccggatct tcacggtagc gcgtttccgg gttaccaatc agacggataa cgccgtcctt
     1441 cagatccttc ataccaccaa cgtaatcacg gacggtaaaa tccgctacgc tgtaatacag
     1501 gctgttgata gtgaaatcgc ggcgctgggc gtcttcttcg atggagccga aaatgttgtc
     1561 gcgcagcaac atgccgtttt gcccgcgttg ggaggtcgtg cggtcgctga cgttaccttc
     1621 gtggtgtcca cggaaggtcg caacttcgat aatctccggg ccaaacatta catgagccag
     1681 acggaaacgg cgacccacca ggcggcagtt acggaacagt ttgcgcacct gctcaggcgt
     1741 ggcgttagtg gttacgtcaa aatctttcgg ctttttgcca agtaacaggt cgcgcacgcc
     1801 gccgccaacc agccaggctt cgtatcccgc tttgttgagc ctgtacatta ccttcagggc
     1861 attttcactg atatctttgc gggaaatagc atgctgctca cgcgggatca ccgtcacctg
     1921 tggacgggcg actgcctgtt cagcctcgct ttcctcgcgg cttagcacct tgcggcaaaa
     1981 attagcgact cgggtaaaaa tagtacacct cggtagtgtc aaacatcatt caggacaaaa
     2041 aaatagcggc taatcatagc tcagcatgac gcatttgaga atgttgaatt tacaattgcc
     2101 gactcgggca cggcggtcag catccagttt ttgacggctg actgaaggat ttgctcgacg
     2161 ctgaaatcct gccagtgtgc ttctgcctgc tgccccagaa attgaagtgc cgcgattagt
     2221 accgggcgtg gatcgccttt cggcaacgca ggcgcatgat tctgcttgga aagtttagcg
     2281 ccttgtggat taagcgccag cggcagatga atgtaatctg gcactttcca gccaaaaagc
     2341 tggtacagcg ggatttgcct tactgttggt tcaatcagat cagccccacg cactatttct
     2401 gtaacgccct ggaaatgatc atcaaccaca acagccaggt tgtaggcgaa caacccatca
     2461 cggcgatgaa tgataaaatc ttcccgtgcc agtttttcgt cggcgtgaat aatgcctcgc
     2521 agcaggtcag taaattgcgt gaccggatgc tgctggcgga tacgcactgc ggcgttgtct
     2581 ggtccatgat gcaacacccg gcaatgaccg tcgtaaatac cgccaatgct ttgaatacgc
     2641 gcacgcgtac aggtgcagta ataacttagt ccttgttcat gtaaccaggc gagtgcttca
     2701 cgataggcgt cgtgacgttg cgattgccag agaacatcgc cgtcccagtg cagaccgtaa
     2761 tgttccagct ggcgcaggat agtttctgcg gcaccgggaa cttcacgagg cgggtcgata
     2821 tcttctatgc gtaccagcca gcgaccttgc cgggcgcgag cctgcaaata gctgccgagc
     2881 gcggcgatca gagagccaaa atgaagctcg ccggaaggag agggggcgaa gcggccaata
     2941 tactgtgtgt ctgtcatctc tttgaacaaa aaataaggcg ggagcatttc ccgcctgtgg
     3001 taaacgtgat ggaacggctg taattagcca gccatctgtt tttcgcgaat ttcagccagc
     3061 gttttgcagt cgatgcacag atcggctgtc gggcgcgctt ccagacggcg aataccaatt
     3121 tcaacaccgc aggattcgca gtagccgaaa tcttcgtctt ccactttttt cagcgtcttc
     3181 tcgatctttt tgatcagctt acgctcgcga tcgcggttac gcagttcgag gctgaactct
     3241 tcttcctggg ctgcacggtc taccgggtcc gggaagttgg ctgcttcatc ctgcatatgt
     3301 gtaacggtgc gatcgacttc atccctgagt tgattacgcc atgcttccag aatacgacgg
     3361 aagtgcgcca gctgggcttc attcatatac tcttcgcccg gcttctcctg atatggttcc
     3421 accccagcga tggcgagaat actcagggac gatgttttac ggttttgccc ttcttgcatg
     3481 ttgcttctcc ttaacacgca ctatcgatcc ccatgttcgg gggaaaaatg aggccgctat
     3541 aaatagcaga tgcttttccg gatagcaatt atctaaacgt aacacttgac aactgtgtga
     3601 ggaaaagcgt atttgcgcac gcgaccagaa tgtaaattaa ccagttactt actttactac
     3661 aatgtaaccg gcagtgattt tttaagagcc atgccttcag cagaaatttc cgctttgtaa
     3721 gccagaattt ctacccccct ctgttgagct tctgacaata gttgcgcgta tttctcatcg
     3781 atgtggcgcg cgggtgaaaa ccgtgtaatg gctgaatgca gcacggcgaa aaaaataacc
     3841 gcacgctggc cttcagccgc tacgctcatc aactcccgaa ggtgtttctg acctcgttca
     3901 gtgaccgcat cgggaaaata tccctgttcg ttctccgcta acgtaaccga tttcacttca
     3961 atatagcagt ctggacgcga atccgcctgc aacataaagt caatacggct gcgttcggag
     4021 ccgtatttta cttcgctttt cagcgagcta tagcctgaca gttctgaaat tgattcatta
     4081 aggatagcct ctttcgtcaa cctgttagcc caaagcgtat tgacgcaaat aaatgcgccg
     4141 ctctggcttt gagttaattc ccaggtgtgt gggtatttcc gtttggtgtt gtctgaagtc
     4201 gaataccaga cggtatcgcc aggcgttgca caaccggtca tcgcacccgt attcgggcag
     4261 tgtagcgtta attcgcgacc atcgggtgtg atcacatcgg ctaaaaaacg tttgtaacgc
     4321 tgaattagcg tcgcgcgctg tagaggggga gaaaattcca ttgcgacaat ccttgttatt
     4381 gcgttagcgc ccagcgtttt agcggcgtgt agcgtgtgcg tccacgggca aacgaggagg
     4441 cgtaaagggt gaactccgtc accgcataag accagttaaa acccggcggc gggaatgtca
     4501 ccgcctcgct ggcgtcgcgc aataaggtaa tatgtggatg aaacggacga ttgctttgaa
     4561 aacaaccgct gcgggcagcc tgtgaacgga gcatattcgc cagctggatt aagccgcgtg
     4621 gaggctgacg catccctaac cacaccacac gcgaacgcag ccattgtccg gcgtcatcaa
     4681 gcgtgagtgt gaaaccaggt tgacgaatcc gtccggctaa aagagaaagc gccttctctt
     4741 tctctgcgct cacttcgcct aaaaatgcca gagtcaggtg caaattatcg gcggcgaccg
     4801 gacgtcccgc ctcaggtggg aagtgtgtgg cgcgccaatg gataatctgt tcgcggattt
     4861 ctgcaggtaa gtcgatagca aagaacagac gttgcggttc agacatgtga ggcactcggt
     4921 tatgaattac cgcgatgcta caatgtggcg cgaagaatgt taaccctctg gagcgttttg
     4981 tgtcgtcgtt gcccgttgct gccgtcttac ctgaattact taccgctctc gattgtgcgc
     5041 cgcaggtatt attaagtgcg ccgaccgggg ccgggaaatc aacctggctg ccgctgcaac
     5101 tgctggcgca tcccggcatt aacgggaaaa ttatcctgct ggagccgcgt cgtctggcgg
     5161 cgcgtaacgt cgcgcaacgg ctggcggagc tgcttaacga aaagccaggc gataccgttg
     5221 gctaccggat gcgtgcgcaa aactgcgtcg ggctgaatac ccgcctggaa gtggttaccg
     5281 aaggcgtgct gacgcggatg atccagcgtg acccggaact gagcggtgtt ggattggtga
     5341 tccttgatga gtttcatgag cgcagtttgc aggcggattt ggcgttggcg ctgttactcg
     5401 atgtgcaaca aggtctgcgt gatgacctta aactgctgat tatgtcggct acgctggaca
     5461 atgaccgctt gcagcaaatg ctgccggaag cgcccgtcgt catctcagaa gggcgctcgt
     5521 ttccggttga acgccgttat ttaccgctgc ccgcgcatca gcgttttgac gaagccgttg
     5581 cggtagccac cgccgaaatg gtgcgtcagg aaagtggatc attactgtta tttttacctg
     5641 gcgtcggaga aattcagcgc gtgcaggaac aactggcttc gcgcatcggc agtgatgtgg
     5701 tgctctgccc gctgtatggc gtgttgtcac tgaacgatca gcgaaaagcg atcctcccgg
     5761 caccgcaagg gatgcgcaaa gtggtgctgg cgaccaatat tgctgaaacc agtttaacca
     5821 tcgaaggtat tcgtctggtg gtggattgtg cccaggagcg tgtggcgcgc tttgatccgc
     5881 gcacggggct tacgcggttg attactcaac gcgttagtca ggcatccatg acgcagcgtg
     5941 ccgggcgcgc cgggcgtctg gagccgggta tcagcctgca tttaatcgcc aaagaacaag
     6001 cagaacgcgc cgcggcgcaa agtgagccga agatcttaca aagcgatctt tccggtttgc
     6061 tgatggaatt actgcaatgg ggatgcagcg atccggcgca gatgagctgg ctggatcaac
     6121 cgccaacggt gaatctactg gccgcgaaac gcctgttaca aatgctgggg gcgctggagg
     6181 gtgaacggct tagtgcgcaa gggcaaaaaa tggcagcgct gggtaacgat ccgcgtttag
     6241 cggcaatgct ggttaacgca aagagcgatg acgaagctgc taccgcggca aaaattgccg
     6301 ccattctcga agagccgcca cggatgggca atagtgacct gggcgtggcg ttttcgcgca
     6361 atcaaccagc ctggcagcaa cgtagtcagc aactgttaaa acgcttaaac gtacgcggcg
     6421 gtgaggcaga cagttcgctt atcgcgccgc tacttgccgg ggcgtttgcc gatcgcattg
     6481 ctcatcgccg tgggcaagat ggacgctatc aactggcaaa cagcatggga gcgatgctcg
     6541 atgccgacga cgcgctaagc cgccacgaat ggttgatcgc accgttatta ttgcagggca
     6601 gcgcctcgcc ggatgcgcgg attttactgg cgctaccggt cgatattgat gaattagtac
     6661 aacgctgtcc gcagctggtg cagcaatctg acaccgtgga atgggatgac gcgcaaggta
     6721 cgctgaaagc ctggcgtcgg ctacaaatcg gtcagttgac ggtgaaagtg cagccgctgg
     6781 cgaaaccgtc agaagacgag ttgcatcagg cgatgcttaa tggcatccgt gataaaggtt
     6841 taagcgtgct caactggacg gcggaagcgg aacagctacg cttgcgtttg ttatgcgccg
     6901 caaagtggtt gccggaatat gactggccag cggttgatga tgaaagtttg ttggcgacgc
     6961 tggaaacgtg gctgctgcca catatgaccg gcgtacattc actacgcggc ctgaaatcac
     7021 tcgacattta tcaggcacta cgcggattac ttgattgggg aatgcagcaa cgtctggata
     7081 gtgaattgcc tgcgcattac actgtgccga cgggaagccg gatcgccatt cgttatcatg
     7141 aagataaccc gcccgcgctg gcggtgagaa tgcaggagat gtttggtgag gccactaatc
     7201 cgacgatcgc ccaggggcgc gtgccgctgg tgctgaagtt gctttcccct gcccaaaggc
     7261 cgctgcaaat cacgcgtgat ttgggggcct tctggaaagg ggcgtatcgt gaggtgcaaa
     7321 aggagatgaa agggcgttat cccaaacacg tctggccgga cgacccggca aacacggcac
     7381 cgacgcgacg gacgaaaaag tattcgtaag gttggttttc tccctctccc tgtgggagag
     7441 ggccggggtg agggcatcag cgcgcacgtt caccctcacc ctaaccctct ccctcaaggg
     7501 agaggggacc gatcgagcgc aaattttgag agatatcttc ttctgtcttg taacagaaga
     7561 acagaaaatc gggcttttgc gcctgaatat tgcggagaaa aagcatggcc gggaatgacc
     7621 gcgagccaat tggacgcaaa gggaaaccga cgcgtccggt caaacaaaag gtaagccgtc
     7681 gtcgttacga agatgacgat gattacgacg attatgatga ctatgaggat gaagaaccga
     7741 tgccgcgcaa aggtaagggc aaaggcaaag ggcgtaagcc tcgtggcaaa cgcggctggc
     7801 tatggctact gctaaaactg gctatcgttt ttgccgtgct gatcgccatt tacggcgttt
     7861 atctcgatca aaaaattcgt agccgtattg atggcaaggt ctggcaactg cccgcggcag
     7921 tttatggccg aatggtcaac cttgaaccag atatgaccat cagcaagaac gaaatggtga
     7981 agctgctgga ggcaacacag tatcgtcagg tgtcgaaaat gacccgtcct ggcgaattta
     8041 ccgtgcaggc caacagcatt gagatgatcc gccgtccgtt tgatttcccg gacagtaaag
     8101 aagggcaggt gcgtgcgcgt ctgacctttg atggtgatca tctggcgacc atcgtcaata
     8161 tggagaacaa ccgtcagttc ggtttcttcc gtcttgatcc gcgtctgatc accatgatct
     8221 cctcgccaaa cggtgagcag cgtttgtttg tgccgcgcag tggtttccct gatttgctgg
     8281 tggatacttt gctggcgaca gaagaccgtc atttttacga gcatgatgga atcagtctct
     8341 actccattgg gcgtgcggtg ctggcaaacc tgaccgcagg tcgcacggtg cagggtgcga
     8401 gtacgctgac gcaacagctg gtgaaaaacc tgttcctctc cagcgagcgt tcttactggc
     8461 gtaaagcgaa cgaagcttac atggcgctga tcatggacgc gcgttacagc aaagaccgta
     8521 ttcttgagct gtatatgaac gaggtgtatc tcggtcagag cggcgacaac gaaatccgcg
     8581 gcttcccgct ggcaagcttg tattactttg gtcgcccggt agaagagcta agcctcgacc
     8641 agcaggcgct gttagtcggt atggtgaaag gggcgtccat ctacaacccg tggcgtaacc
     8701 cgaaactggc gctggagcga cgtaatctgg tgctgcgtct gctgcaacag caacagatta
     8761 ttgatcaaga actctatgac atgttgagtg cccgtccgct gggggttcag ccgcgtggtg
     8821 gggtgatctc tcctcagcca gcctttatgc agctggtgcg tcaggagttg caggcaaaac
     8881 tgggcgataa ggtaaaagat ctctccggcg tgaagatctt cactaccttt gactcggtgg
     8941 cccaggacgc ggcagaaaaa gccgccgtgg aaggcattcc ggcactgaag aaacagcgta
     9001 agttgagcga tcttgaaact gcgattgtgg tcgtcgaccg ctttagtggt gaagttcgtg
     9061 cgatggtcgg aggttctgag ccgcagtttg cgggctacaa ccgtgcgatg caggcgcgtc
     9121 gttcgattgg ttcccttgca aaaccagcga cttatctgac ggctttaagc cagccgaaaa
     9181 tctatcgcct gaacacgtgg attgccgatg cgccaatcgc gctgcgtcag ccgaatggcc
     9241 aggtctggtc accgcagaac gatgaccgtc gttatagcga aagcggcaga gtgatgctgg
     9301 tggatgcgtt gacacgttcg atgaacgtgc cgacggtaaa tctggggatg gcgctggggc
     9361 tgcctgcggt tacggagacc tggatcaaac tgggcgtacc gaaagatcag ttgcatccgg
     9421 ttccggcaat gctgctgggg gcgttgaact taacgccaat cgaagtggcg caggcattcc
     9481 agaccatcgc cagcggtggt aaccgtgcac cgctttctgc actgcgttcg gtaatcgcgg
     9541 aagatggtaa ggtgctgtat cagagcttcc cgcaggcaga acgcgctgtt ccggcgcagg
     9601 cggcgtatct gacactatgg accatgcagc aggtggtaca acgtggtacg ggtcgtcagc
     9661 ttggggcgaa atacccgaac ctgcatctgg cagggaaaac agggactacc aacaataacg
     9721 tagatacctg gtttgcaggc attgacggca gcacggtggc catcacctgg gtaggccgtg
     9781 ataacaacca gccgaccaaa ctgtatggag ccagcggggc aatgtcgatt tatcagcgtt
     9841 atctggctaa ccagacgcca acgccgctga atcttgttcc gccagaagat attgcagata
     9901 tgggcgtgga ctacgacggc aactttgttt gcagcggtgg catgcgtgtc ttgccggtct
     9961 ggaccagcga tccgcaatcg ctgtgccagc agagcgagat gcagcagcag ccgtcaggca
    10021 atccgtttga tcagtcttct cagccgcagc aacagccgca acagcaacct gctcagcaag
    10081 agcagaaaga cagcgacggt gtagccggtt ggatcaagga tatgtttggt agtaattaac
    10141 atctaagcgt gaaataccgg atggcgagtt gccatccggt aaaataacat cccatctaag
    10201 atattaaccc tttcttttca tctggttgtt tattaaccct tcaggaacgc tcagattgcg
    10261 taccgcttgc gaacccgcca gcgtttcgaa tattatctta tctttataat aatcattctc
    10321 gtttacgtta ttattcactt tacatcagag atataccaat ggcgcgttcc aaaactgctc
    10381 agccaaaaca ctcactgcgt aaaatcgcag ttgtagtagc cacagcggtt agcggcatgt
    10441 ctgtttatgc acaggcagcg gttgaaccga aagaagacac tatcaccgtt accgctgcac
    10501 ctgcgccgca agaaagcgca tgggggcctg ctgcaactat tgcggcgcgg cagtcagcta
    10561 ccggcactaa aaccgatacg ccgattcaaa aagtgccaca gtctatttct gttgtgaccg
    10621 ccgaagagat ggcgctgcat cagccgaagt cggtaaaaga agcgcttagc tacacgccgg
    10681 gtgtctctgt tggtacgcgt ggcgcatcca acacctatga ccacctgatc attcgcggtt
    10741 ttgcggcaga aggccaaagc caaaataact atctgaatgg cctgaagttg cagggcaact
    10801 tctataacga tgcggtcatt gacccgtata tgctggaacg cgctgaaatt atgcgtggcc
    10861 cggtttccgt gctttacggt aaaagcagtc ctggcggtct gttgaatatg gtcagcaagc
    10921 gtccgaccac cgaaccgctg aaagaagttc agtttaaagc cggtactgac agcctgttcc
    10981 agactggttt tgactttagc gatgcgttgg atgatgacgg tgtttactct tatcgcctga
    11041 ccggtcttgc gcgttctgcc aatgcccagc agaaagggtc agaagagcag cgttatgcta
    11101 ttgcaccggc gttcacctgg cgtccggatg ataaaaccaa tttcaccttc ctttcttact
    11161 tccagaacga gccggaaacc ggttattacg gctggttgcc gaaagaggga accgttgagc
    11221 cgctgccgaa cggtaagcgt ctgccgacag actttaatga aggggcgaag aacaacacct
    11281 attctcgtaa tgagaagatg gtcggctaca gcttcgatca cgaatttaac gacaccttta
    11341 ctgtgcgtca gaacctgcgc tttgctgaaa acaaaacctc gcaaaacagc gtttatggtt
    11401 acggcgtctg ctccgatccg gcgaatgctt acagcaaaca gtgtgcggca ttagcgccag
    11461 cggataaagg ccattatctg gcacgtaaat acgtcgttga tgatgagaag ctgcaaaact
    11521 tctccgttga tacccagttg cagagcaagt ttgccactgg cgatatcgac cacaccctgc
    11581 tgaccggtgt cgactttatg cgtatgcgta atgacatcaa cgcctggttt ggttacgacg
    11641 actctgtgcc actgttcaat ctgtacaatc cggtgaatcc cgatttcgac ttcaatgcca
    11701 aagatccggc aaactccggc ccttaccgca ttctgaataa acagaaacaa acgggcgttt
    11761 atgttcagga tcaggcgcag tgggataaag tgctggtcac cctaggcggt cgttatgact
    11821 gggcagatca agaatctctt aaccgcgttg ctgggacgac cgataaacgt gatgacaaac
    11881 agtttacctg gcgtggtggt gttaactacc tgtttgataa tggtgtaaca ccttacttca
    11941 gctatagcga atcgtttgaa ccttcttcgc aagttgggaa ggatggtaat attttcgcac
    12001 cgtctaaagg taagcagtat gaagtcggcg tgaaatatgt accggaagat cgtccgattg
    12061 tagttactgg tgccgtgtat aatctcacta aaaccaacaa cctgatggcg gaccctgagg
    12121 gttccttctt ctcggttgaa ggtggcgaga tccgcgcacg tggcgtagaa atcgaagcga
    12181 aagcggcgct gtcggcgagt gttaacgtag tcggttctta tacttacacc gatgcggaat
    12241 acaccaccga tactacctat aaaggcaata cgcctgcaca ggtgccaaaa cacatggctt
    12301 cgctgtgggc tgactatacc ttctttgacg gtccgctttc aggtctgacg ctgggcaccg
    12361 gtggtcgtta tactggctcc agctatggtg atccggctaa ctcctttaaa gtgggaagtt
    12421 atacggtcgt ggatgcgtta gtgcgttatg atctggcgcg agtcggcatg gcgggctcca
    12481 acgtggcgct gcatgttaac aacctgttcg atcgtgaata cgtcgccagc tgctttaaca
    12541 cttatggctg cttctggggc gcagaacgtc aggtcgttgc aaccgcaacc tttcgtttct
    12601 aatttctctt ttggggcacg gatttccgtg cccatttcac aagttggctg ttatgcagga
    12661 atacacgaat cattccgata ccacttttgc actgcgtaat atctcctttc gtgtgcccgg
    12721 gcgcacgctt ttgcatccgc tgtcgttaac ctttcctgcc gggaaagtga ccggtctgat
    12781 tggtcacaac ggttctggta aatccactct gctcaaaatg cttggtcgtc atcagcctcc
    12841 gtcagaaggg gagattcttc ttgatgccca gccgctggaa agctggagca gcaaagcgtt
    12901 tgcccgcaaa gtggcttatt tgccgcagca gcttcctccg gcagaaggga tgaccgtgcg
    12961 tgaactggtg gcgattggtc gttacccgtg gcatggcgcg ctggggcgct ttggtgcggc
    13021 agatcgcgaa aaggtcgagg aagctatctc gctggttggc ttaaaaccgc tggcgcatcg
    13081 gctggtcgat agtctctctg gcggcgaacg tcagcgggcg tggatcgcca tgctggtggc
    13141 gcaggatagc cgttgtctgt tgctcgacga accgacttca gcgctggata tcgcccacca
    13201 ggttgatgtg ctggcgctgg tgcaccgttt aagtcaggaa cgtggcctga cggtcattgc
    13261 cgtgttgcac gatatcaata tggcggcacg ctactgtgat tatctggtcg ccctgcgcag
    13321 tggtgaaatg attgctcagg gaacgcctgc ggaaattatg cgcggcgaaa ccctcgaaat
    13381 gatttatggc atcccgatgg gtattttgcc gcatccggcg ggtgctgcac ctgtgagttt
    13441 tgtttattga tgagcggctt acctcttatt tcgcgccgtc gactgttaac ggcgatggcg
    13501 ctttctccgt tgttatggca gatgaatacc gcccacgcgg cggctattga ccccaatcgt
    13561 attgtggcgc tggagtggtt gccggtggaa ttactgctgg cgctcggcat cgtgccttac
    13621 ggcgtggcgg ataccatcaa ctatcgcctg tgggtcagcg aaccaccatt gccggactca
    13681 gtgattgacg tcggtttgcg tacagaacct aaccttgaac tgctgaccga aatgaaaccg
    13741 tcgtttatgg tctggtcggc aggatatggc ccttcatcag aaatgctggc acgtattgcg
    13801 ccgggtcgcg gatttaactt cagtgacggc aaacatccgt tggcgatggc gcgtaaatcg
    13861 ctgacggaaa tggcagattt acttaacctg caaagcgcag cggaaacgca tttagcgcaa
    13921 tacgaagact ttatccgcag catgaaaccc cgctttgtga agcgtggtgc acgcccgtta
    13981 ctgctgacga cgcttatcga tccgcgccat atgctggtct tcggtccaaa cagcttgttc
    14041 caggaaattc tcgatgagta cggcatccca aatgcctggc aaggggaaac caacttctgg
    14101 ggcagtaccg ccgtcagtat cgatcgtctg gcagcgtata aagacgttga tgtgctctgt
    14161 tttgatcacg acaacagcaa agacatggat gcgctaatgg caaccccgct gtggcaggcc
    14221 atgccgtttg tccgtacagg gcgctttcag cgcgtacccg cagtctggtt ttatggtgca
    14281 acgctttcgg caatgcactt tgtgcgtgtt ctggataacg ccatcggagg taaagcgtga
    14341 gtaaacgaat tgcgcttttc ccggcgttat tgctggcgct gttagtgatt gtcgctacgg
    14401 cgctcacctg gatgaacttc tcacaggcgc tgccgcgcag tcagtgggcg caggccgcct
    14461 ggtcgccgga tattgacgtc atcgagcaga tgatttttca ctacagcttg ttgccgcgtc
    14521 tggcgatttc gctgctggtg ggcgcgggcc tggggctggt gggcgtgctg tttcagcaag
    14581 tactgcgtaa cccgctggcg gaaccgacga cgttgggcgt tgcaacaggc gcgcaactgg
    14641 ggattaccgt caccacgctc tgggcgatcc ctagcgcgat ggcgagccag tttgctgcgc
    14701 tggcaggggc ttgcgttgtt ggcttaattg tctttggcgt cgcgtggggg aaacggctgt
    14761 cgccggtaac gctgattttc gcggggttgg tagtgagcct ttattgcggc gcaatcaatc
    14821 agttactggt tatcttccat catgaccagt tgcaaagcat gtttctgtgg agcactggaa
    14881 cgctgacgca aaccgactgg ggcggtgttg agcgtttatg gccgcagttg ctgggcggtg
    14941 tgatgctgac gttgctgcta cttcgtccgt taaccctgat ggggcttgat gatggcgtgg
    15001 cgcgcaatct cgggctggcc ttgtcgcttg cgcgtctggc ggcgctgtcg ctggcgattg
    15061 tcatcagtgc gctgctggtg aacgctgtgg ggattatcgg ctttatcggg ttgttcgcgc
    15121 cgctgctggc aaaaatgctg ggggcgcggc gtctgctgcc acgactgatg ctggcgtcgt
    15181 tgattggtgc gctgatcctc tggctttccg atcaaatcat cctctggctg actcgcgtgt
    15241 ggatggaagt gtccaccggt tcggtcactg cgttgatcgg tgcgccgctg ctactgtggc
    15301 tgctgccgcg tttacgcagc attagcgcgc cggatatgaa ggttaacgat cgtgtcgcga
    15361 ctgaacgcca acatgtgctg gcgtttgccc tcgcgggcgg cgtgctgttg ttgatggctg
    15421 tggtggtggc gctgtcgttt ggtcgtgatg cgcacggctg gacgtgggcg agcggggcgt
    15481 tgctcgagga tttaatgccc tggcgctggc cgcgaattat ggcggcgctg tttgcgggcg
    15541 tcatgctggc ggtagcgggc tgtattattc agcgactgac cggcaacccg atggcaagcc
    15601 cggaagtgct ggggattagc tccggcgcgg cgtttggtgt ggtgttaatg ctgtttctgg
    15661 taccgggtaa tgcctttggc tggttgttac ccgcaggcag tctcggggcg gcggtgacgc
    15721 tgttgatcat tatgatcgcc gccggacgcg gtggattttc cccacaccgc atgttactgg
    15781 cggggatggc gttaagtacc gcgttcacca tgcttttgat gatgttgcag gcaagtggtg
    15841 acccgcgaat ggcgcaagtg ctgacctgga tttccggttc gacctacaac gcgaccgatg
    15901 ctcaggtctg gcgcaccgga attgtgatgg tgattttgct ggcgattact ccgctgtgcc
    15961 gccgctggct gaccatttta ccgctgggtg gtgataccgc gcgggcggta gggatggcgc
    16021 tgacgccgac gcgaattgcg ctgctgctgt tagcggcttg cctgacggcg accgcgacga
    16081 tgactattgg accgttgagt tttgttggtt taatggcacc gcatattgcg cggatgatgg
    16141 gctttcgacg gacgatgcca cacatcgtaa tttcggcgct ggtgggtggt ttactgctgg
    16201 tgttcgctga ctggtgtggg cggatggtgc tgtttccatt ccagatcccg gcggggctgc
    16261 tgtcgacctt tatcggtgcg ccatatttta tctatttgct gagaaagcag agccgttaat
    16321 agaatgcctc gcccggtggg cgaggcaaaa ttatcacaac ttcgcgaaca cccgacgtgc
    16381 agcatcgatg gtgttattga tatcttccat gctgtgcgcc acagacataa agcctgcttc
    16441 aaacgctgac ggtgccaggt aaacaccttc atccagcatc atatggaaga aacgcttaaa
    16501 acgctcgacg tcacaggcca tcacatcctg atagcacgtc acggactcgg cgtcggtaaa
    16561 gaagataccg aacataccac cgacgtggtt aacgaccagc ggaattcccg cttcttctgc
    16621 cgcttccagc agaccttctg ccagacgtgt tgtcagctca tccagcgttt cgtgaatgcc
    16681 cggctgcgcg acttcattca gacaggcgaa acccgctgcc atcgcaatcg ggttaccgga
    16741 aagcgtaccc gcctgataga ccggacctgt cggggccagc gcatccatta catcacgacg
    16801 accaccgaat gcgcctaccg gcattccacc gccgatgatt tttccgaggc aggttaaatc
    16861 tggcactacg gcgaaataat cctgtgcgcc agctagcgct acgcggaaac cggtcatcac
    16921 ttcatcgatg atcagcaacg cgccaaattc gtcgcacagc gcacgcagac ctggcaggaa
    16981 ctctggcagc ggcggaacac agttcatatt gcctgccacc ggctcgacga taatacaggc
    17041 aatctcttgc gggtattgct caaacgcggc gcgtacagag gccagatcgt tataagtaca
    17101 ggttaaggta tgtttggcga aatctgccgg aacgcccggc gagtttggct ggcctaacgt
    17161 gagtgcgcca gaaccggctt tcaccagcag gcagtcagcg tgaccgtggt aacaaccttc
    17221 aaatttaatg attttgtcgc gaccggtaaa accacgggcc aggcggatgg cgctcatcgt
    17281 cgcctcggtg ccggagttca ccatgcgcac catatccatg gtcgggacca gttcggtcac
    17341 cagttgcgcc attttcactt ccatttcggt cggtgcacca aagcttaaac cacgctcggc
    17401 ggcttcaatc acggcattgc ggatcgccgg atggttatgg cccagcacca tcggccccca
    17461 ggaaccgaca taatcgatat aggctttgcc atcaacatcg tacagataag cgccgtccgc
    17521 tttttcgata aacagtggag tgccgcccac gccagtaaag gcgcgaacag gggagttcac
    17581 accgccaggg atcagctcgc gcgctgcgct gtaaagattt tcagacttac tcatggaggg
    17641 ttcctgattc gtagaaaaag tgaatggctg ctattccatg ttattcataa caagttaaat
    17701 actcgtcaaa catcaggctg cttgtactgg tgcaatcctg atttcttaga gtataaaagt
    17761 tttgtgcatt tgaaacatta cgctttgcaa aggattttca tggaacgtgc gagtaaaatg
    17821 ccgtcatctt atttgtatga ccaataagtg atcattggat gaaaactgat actccctctt
    17881 tagaaacacc gcaggccgcg cgcctgcgac gcagacaact gattcgccaa cttcttgagc
    17941 gcgataaaac cccgttagcc attttgttta tggcggcagt cgtcggcacg cttgtcgggc
    18001 tggctgcggt tgcttttgac aaaggtgtcg cctggttgca gaaccaacgt atgggggcgc
    18061 tggtacatac tgctgataat tatccgctac tgttaaccgt cgcttttctc tgttcggcgg
    18121 tgctggcgat gttcggctac tttttggtgc gcaaatacgc gcccgaagca gggggatcgg
    18181 ggatcccgga aattgaaggg gcgctggaag atcaacgtcc tgttcgctgg tggcgtgtat
    18241 tgccggtgaa attctttggc gggctgggga cactcggcgg aggcatgggg ctagggcgcg
    18301 aaggaccaac cgtgcagatc ggcggcaata tcagtcgcat ggtgcttgat attttccgcc
    18361 tgaaaggtga tgaagctcgc catacgctgc tggcaaccgg tgctgctgcg gggctggctg
    18421 cggcctttaa cgcgccgctg gcgggtattt tgtttattat cgaagagatg cgtccgcagt
    18481 ttcgctatac gttaatttcg attaaagcgg tatttattgg cgtcattatg tcgaccatta
    18541 tgtaccggat ttttaatcat gaagttgcgt tgattgacgt cggtaaactt tctgatgcgc
    18601 cgcttaatac gctgtggctt tatctgatcc tcggtattat ttttggcatt ttcggcccta
    18661 tttttaataa atgggtgctg gggatgcagg atttgctgca ccgtgtgcac ggcggcaata
    18721 ttaccaaatg ggtactgatg gggggggcga ttggcggtct gtgtggattg ctggggtttg
    18781 tggcaccagc aacgtcgggc ggcggtttta acctgattcc tatcgctacc gcggggaatt
    18841 tcagcatggg aatgctggtg tttatcttcg tcgctcgggt cattaccacc ttactctgct
    18901 tctcttccgg cgcgccgggc ggtatttttg ccccgatgct ggcgctgggt actgtgctgg
    18961 gaaccgcttt cggaatggtt gccgttgagc tgtttccgca atatcacctg gaggcgggga
    19021 cgtttgctat tgccggaatg ggggcattac tggcggcatc tattcgcgcg ccgttaacgg
    19081 ggatcattct ggttctggag atgaccgata actaccagct cattttgcca atgattatta
    19141 ccggtcttgg cgcaacacta ttagcgcaat ttaccggcgg gaaacctcta tactcggcga
    19201 ttcttgcgcg cacgctggca aaacaggaag ctgagcaact ggcgcgaagc aaggccgcat
    19261 cagccagcga gaatacttga acgaaatacc agggtattag ataatggcga ttattattgg
    19321 gttagaattt gcccgattgc cgatgtcgtt tggagcaaaa tatgagtgat gacgtagcac
    19381 tgccgctgga gtttaccgac gcagcagcca acaaagttaa aagcctgatc gctgacgaag
    19441 ataacccgaa tctgaaatta cgcgtgtata tcaccggtgg cggttgcagc ggcttccagt
    19501 atggtttcac ctttgatgat caggtgaacg aaggcgatat gaccatcgaa aaacagggcg
    19561 ttggcctggt ggttgatccg atgagcctgc aatatctggt cggcggttcc gttgactaca
    19621 ccgaaggact ggaaggttct cgtttcatcg tgaccaaccc gaacgcgaaa agcacctgcg
    19681 gttgcggttc ttcctttagc atctaatctg ttgttcatgt gccggataaa ctatccggca
    19741 catcttctct cttcaacgcc cattctcatc cagcgcaaac gtcggtagct taagatgcca
    19801 acgaatagcc gccagccgaa tcaatagcgt cacgaccatg cccatcatac tggctgtttc
    19861 cagtggtacg gaaaatgtgt aataagccgt agcgtggaca ataccgccga taatacaggc
    19921 agttgcgtag atttctgtac gtaaaatcat ggggatttcg cggaccagta catcacgaat
    19981 gatcccgccg ccaacgccag taatgacgcc catacaaacc gcgattaacg gaccggcttc
    20041 cgcattaaag gctttattca cgctaatgcc gacaaacacc gccagaccaa cggcgtccag
    20101 caccggcaac atccattttg gtaagcgtct tggctggcgc accagcacga tggtcagcat
    20161 gctggtgacc atggcaacga ccagatcggt gggatctttc acccaaaata ccgggccgtg
    20221 atccagcgcc atgtcgcgaa ttgttccgtc gcctactgcg gtaaccacgc ccagtaccag
    20281 aacaccaaaa gggtccatac gcaattttcc ggctaacaaa acgccgtaga tggcaaatac
    20341 cgctgtgccg actatatcca gccaatagac gagcattgtt caatccccac tgagcacctg
    20401 aaaaggtcag gcactaatct acctgtgaaa gcgcattaca gagctgttgt gctgcgagga
    20461 taatacgtgg gcttgcacgt tcaaaccagt cactcgtgag aggaataacg ggaattttga
    20521 gctgttcacc ccagtattgt ttgattttag gaatttggtc cggtccgcct gtaatgacaa
    20581 tagcctgtgg cgagcgtgct aacacctgtt cgcggctaac ttgcggccag ggaacccggc
    20641 tgtctttaaa gatgttttct ccgccacaaa cttcgagtac ctggttctga atcgactctt
    20701 ttccactggt aaatggcgga ttaatgccga attgcagaaa aacacgtttt ttaggtttat
    20761 cagcatattg cgctttcaat tgcacgtact gatccagcag ggattgcgcg gcttgttcgg
    20821 ccttgtctgg ttgcggactc cagggggcca gttgacgtaa cgcattggca atttgttcaa
    20881 tgcttgtcgc atcgacccac atcactttta ttcccagcga agccagctgg tcaacctgcc
    20941 gctcggcatt acctccacgc caggcaatca ccagatcggg tttcagcgcg acaatgcgtt
    21001 ccagattcat cccctgccag gtggaaacct gctcaatctt ttgcgcttgt ggaggatagt
    21061 cggaatagct gctgacccca accggcgtga tcccggcggc aaaggcaagt tcagtgttgg
    21121 cgggagaaag cgtgatgacg cgcggcgcgg cgttgagcca cagtggcgcg agaaaagaca
    21181 gggcgaccag cgccctgaac agtgatttag ccatgtgcca gtttctgcac cagtgactca
    21241 accatcaggc tggactgttt agcggcaaca gccaggaact catcgaagct aagatgagac
    21301 tgttgatcgg ccacgtcgga gatggcgcgt acgacaacaa acgggacgtt gaaattgtgg
    21361 cagacatggg cgattgccgt cgcttccatc tctacagcaa tggcctgtgg gaagttgtgg
    21421 cggattttcg ccagaccaac agaaccgttg atgaaagcgt cgccgctaac aatcaggcca
    21481 cgtacagcgt taagattcag ttcggcaatg caggcctcag cggcagcgat cagtttatcg
    21541 tcagctttaa agcctgccgg acagcctggt aactgaccgt attcataacc aaatgccgtg
    21601 acatccgcgt cgtgataacg cgcttcgtcc gagacaacga tatcgcccac tttcagcgtt
    21661 ggtgccaggc caccggcaga accggtgtta ataatcacat ctggcttgca gtgttccaac
    21721 agcagagtgg cacccagcgc cgcagcgact ttaccgatgc ccgatttcag aagcgcaacc
    21781 tcggttccat tcagttggcc ggtatagatt tcgcaaccgc cgagactgat agtttgacgg
    21841 ttttcgattt tgtcacgcag cagcgtaact tcttcttcca ttgcaccaat gatgccgatt
    21901 ttcatagatt tactcgcgat aagcccgatt tgaaggcata gtttaccatg cgcttacggg
    21961 gaagcgtatt tctcacgcgg gagaggacat ggcacagatt gatttccgaa aaaaaataaa
    22021 ctggcatcgt cgttaccgtt caccgcaggg cgttaaaacc gaacatgaga tcctgcggat
    22081 cttcgagagc gatcgcgggc gtatcatcaa ctctccggca attcgtcgtc tgcaacaaaa
    22141 gacccaggtt tttccactgg agcgcaatgc cgccgtgcgc acgcgtctta cccactcgat
    22201 ggaagtccag caggtgggac gctacatcgc caaagaaatt ttaagccgtc tgaaagagct
    22261 taaattgctg gaagcatacg gcctggatga actgaccggc ccctttgaaa gcattgttga
    22321 gatgtcatgc ctgatgcacg atatcggcaa tccgccgttt ggtcattttg gcgaagcagc
    22381 gataaatgac tggtttcgcc aacgtttgca cccggaagat gccgaaagcc agcctctgac
    22441 tgacgatcgc tgcagcgtgg cggcactacg tttacgggac ggggaagaac cgcttaacga
    22501 gctgcggcgc aagattcgtc aggacttatg tcattttgag gggaatgcac aaggcattcg
    22561 tctggtgcat acattgatgc ggatgaatct cacttgggca caggttggcg gtattttaaa
    22621 atatacccgt ccggcgtggt ggcgtggcga aacgcctgag acacatcact atttaatgaa
    22681 aaagccgggt tattatcttt ctgaagaagc ctatattgcc cgcttgcgta aagaacttaa
    22741 tttggcgctt tacagtcgtt ttccattaac gtggattatg gaagctgccg acgacatctc
    22801 ctattgtgtg gcagaccttg aagatgcggt agagaaaaga atatttaccg ttgagcagct
    22861 ttatcatcat ttgcacgaag cgtggggcca gcatgagaaa ggttcgctct tttcgctggt
    22921 ggttgaaaat gcctgggaaa aatcacgctc aaatagttta agccgcagta cggaagatca
    22981 gttttttatg tatttacggg taaacaccct aaataaactg gtaccctacg cggcacaacg
    23041 atttattgat aatctgcctg cgattttcgc cggaacgttt aatcatgcat tattggaaga
    23101 tgccagcgaa tgcagcgatc ttcttaagct atataaaaat gtcgctgtaa aacatgtgtt
    23161 tagccatccg gatgtcgagc agcttgaatt gcagggctat cgggtcatta gcggattatt
    23221 agagatttat cgtcctttat taagcctgtc gttatcagac tttactgaac tggtagaaaa
    23281 agaacgggtg aaacgtttcc ctattgagtc gcgcttattc cacaaactct cgacgcgcca
    23341 tcggctggcc tatgtcgagg ctgtcagtaa attaccgtca gattctcctg agtttccgct
    23401 atgggaatat tattaccgtt gccgcctgct gcaggattat atcagcggta tgaccgacct
    23461 ctatgcgtgg gatgaatacc gacgtctgat ggccgtagaa caataaccag tcttttgtaa
    23521 agacgaacaa taaattttta ccttttgcag aaactttagt tcggaacttc gggctataaa
    23581 acgaatctga agaacacagc aattttgcgt tatctgttaa tcgagactga aatacatgaa
    23641 aaaaaacaca ttagcactga gtgcactggc tctgagttta ggtttggcgt tatctccgct
    23701 ctctgcaacg gcggctgaga cttcttcagc aacgacagcc cagcagatgc caagccttgc
    23761 accgatgctc gaaaaggtga tgccttcagt ggtcagcatt aacgtagaag gtagcacaac
    23821 cgttaatacg ccgcgtatgc cgcgtaattt ccagcagttc ttcggtgatg attctccgtt
    23881 ctgccaggaa ggttctccgt tccagagctc tccgttctgc caggggggcc agggcggtaa
    23941 tggcggcggc cagcaacaga aattcatggc gctgggttcc ggcgtcatca ttgatgccga
    24001 taaaggctat gtcgtcacca acaaccacgt tgttgataac gcgacggtga ttaaagtcca
    24061 gctgagcgat ggtcgtaagt tcgacgcgaa gatggttggc aaagatccgc gctctgatat
    24121 cgcgctgatc caaatccaga acccgaaaaa actgaccgca attaagatgg cggattctga
    24181 tgcactgcgc gtgggtgatt acaccgtagc gattggtaac ccgtttggtc tgggcgagac
    24241 ggtaacttcc gggattgtct ctgcgctggg gcgtagcggc ctgaatgctg aaaactacga
    24301 aaacttcatc cagaccgatg cagcgatcaa ccgtggtaac tccggtggtg cgctggttaa
    24361 cctgaacggc gaactgatcg gtatcaacac cgcgatcctc gcaccggacg gcggcaacat
    24421 cggtatcggt tttgctatcc cgagtaacat ggtgaaaaac ctgacctcgc agatggtgga
    24481 atacggccag gtgaaacgcg gtgagctggg tattatgggg actgagctga attccgaact
    24541 ggcgaaagcg atgaaagttg acgcccagcg cggtgctttc gtaagccagg ttctgcctaa
    24601 ttcctccgct gcaaaagcgg gcattaaagc gggtgatgtg atcacctcac tgaacggtaa
    24661 gccgatcagc agctttgccg cactgcgtgc tcaggtgggt actatgccgg tgggcagcaa
    24721 actgaccctg ggcttactgc gcgacggtaa gcaggttaac gtgaacctgg aactgcagca
    24781 gagcagccag aatcaggttg attccagctc catcttcaac ggcattgaag gcgctgagat
    24841 gagcaacaaa ggcaaagatc agggcgtggt agtgaacaac gtgaagacgg gcactccggc
    24901 tgcgcagatc ggcctgaaga aaggtgatgt gattattggt gcgaaccagc aggcagtgaa
    24961 aaacatcgct gaactgcgta aagttctcga cagcaaaccg tctgtgctgg cactgaacat
    25021 tcagcgcggc gacagcacca tctacctgtt aatgcagtaa tctccctcaa ccccttcctg
    25081 aatacgggaa ggggttctcc ttacaatctg tgaacttcgc cacaactcca tacatcttca
    25141 tcatcctttg ggcatttgca caatgccgta cgttacgtac ttccttatgc taagccgtgc
    25201 ataacggagg acttatggct ggctggcatc ttgataccaa aatggcgcag gatatcgtgg
    25261 cacgtaccat gcgcatcatc gataccaata tcaacgtaat ggatgcccgc gggcgaatta
    25321 tcggcagcgg cgatcgtgag cgtattggtg aattgcacga aggtgcattg ctggtacttt
    25381 cacagggacg agtcgtcgat atcgatgacg cggtagcacg tcatctgcac ggtgtgcggc
    25441 aggggattaa tctaccgtta cggctggaag gtgaaattgt cggcgtaatt ggcctgacag
    25501 gtgaaccaga gaatctgcgt aaatatggcg aactggtctg catgacggct gaaatgatgc
    25561 tggaacagtc gcggttgatg cacttgttgg cgcaggatag ccgtttgcgg gaagaactgg
    25621 tgatgaacct gattcaggca gaggagaata ctcccgcact tctgaatggg cgcaacggct
    25681 ggggatcgat ctcaatcaac cgcgagtggt ggctattgtt gaggtcgaca gcggtcagct
    25741 tggcgtggac agcgcaatgg cggagttaca acaactgcaa aacgcgctga ctacgcccga
    25801 gcgtaataat ctggtggcga ttgtctcgct aaccgaaatg gtggtgttga aaccggcgtt
    25861 gaactctttt gggcgctggg atgcagaaga tcatcgtaag cgagttgaac aactgattac
    25921 ccgcatgaaa gagtacggcc agctgcgttt tcgcgtttca ctgggcaact attttaccgg
    25981 tcctggcagt attgcccgat cctatcgtac ggcgaaaacg acgatggtgg tgggtaaaca
    26041 gcggatgcca gaaagtcgct gctattttta tcaggatctg atgttacctg tgttactcga
    26101 cagtttgcgt ggcgactggc aggccaacga actggcgcga ccgctggcgc ggctgaaagc
    26161 gatggacaat aacggcttgc tgcgacgaac gctggcggcg tggtttcgcc acaatgtgca
    26221 accgctggca acgtcaaagg cgttgtttat tcatcgtaat accctggagt atcggcttaa
    26281 tcgtatatcg gaactgaccg ggcttgattt gggcaatttt gatgacaggt tgctgctgta
    26341 tgtggcgtta caactggatg aagagcggta gattatgcgt taaggttgag gcggcgctgg
    26401 ctcattcccc tcagcccgaa ggggagaggg gaatgcccga gttaaccgtc aattcgggat
    26461 tatttacgcg ttagtttttc cagatcggct tcaatctcgc tgatcttatt ggttacgact
    26521 gactccaggt gacgtaagtc gtcgaggatc ttacgcttaa gatcaacttc gctgcggtca
    26581 cgctgacaga tttgatcaag ctcatcaatg atataccgta gattcgggct gatttcctgg
    26641 acttctttat aaccctgacc cacaccatca gcgacgaccg ttttacgctg acgcggatat
    26701 ttaaacttaa cgctcttggc gaaaaactcg cctttgtctt tctggaaata gattttcaga
    26761 atatcgttgt tggcttcctg ccggaggctg taacgatcaa tttcttcagg attggtaata
    26821 cccagacttt tcagattgtc gtacatagcg ttacctcaaa atgagtcagt aaattgtgct
    26881 tattttagca tttggcttgg cccgcccggc tcgactgtga tcgcagaaag cctttttctt
    26941 ccagacttct atcatggcgt aaaaatcaaa aaattacctg ctttattgtg gcgatattcc
    27001 atgggatgga ttagcacatt tatcttcgtt ttgcgaatta catgacagtc cgaaacaaga
    27061 tgaaaaagta tttaaaaaag ggggaggaag aatgagtagg acgaattatt ctgagccaga
    27121 aagcatacgg gcagcatcat gctgcccgta tatgtactct taatcgatgg tacgcagcag
    27181 ttcgttaatg ccgactttgc cgcgagtttt cgcgtcaact ttcttaacga taaccgcaca
    27241 gtagaggctg tatttgccat cttttgacgg cagattacct gaaacaacca cagaccccgc
    27301 cggaacgcga ccgtagtgga tttcgccggt ttcacggtcg taaatacggg tgctctgacc
    27361 aatgtatacg cccatagaga tgacggaacc ttcttcgaca atcacccctt caaccacttc
    27421 agagcgtgcg ccaatgaagc agttatcttc aataatggtc gggttagcct gcagcggctc
    27481 cagcacgccg ccgatgccca cgccaccgga aaggtggacg tttttaccaa tctgcgcaca
    27541 agaaccgacg gtcgcccagg tatcaaccat ggtgccttca tcaacatatg cgccgatgtt
    27601 aacgtaagac ggcatcagca cggtgttacg ggcaataaac gcaccctgac gtaccgccgc
    27661 tggtggcaca acgcggaagc cttctttctg gaaacgtgct tcgtcgtagt cggcgaattt
    27721 catcggcact ttgtcgaagt agcggctttc tgccccttcg atcacctgat tatcattaat
    27781 acggaaagag agcagcaccg cttttttcaa ccactgatgc gtcacccact gaccgtcaat
    27841 tttttccgct acacgcagtg cgccggaatc cagcagggcg atcacctgat ttaccgcttc
    27901 gcgggtaacg gtgtctgcat tggctggcgt gatctcggca cggcgttcaa aagcggtttc
    27961 aataatgttc tgtaactgct gcattgttaa actcttttca tatcagtaaa cacatcaccc
    28021 tttatcgttt ggattgaggg cctctgtcaa ccgctgatgc acttcctgct gcaactcgtt
    28081 attaagcgca cgccggtcag cggtggcaat tatgaataaa tcttctactc gctcgccaat
    28141 ggttgtaatt cgggcaccat gaagcgaaat tcccagatcg gcaaaaattt tcccgactcg
    28201 cgccagcagt ccaggttggt cgagggcgat cagttcgagg aacgatttgc ggtcggtatg
    28261 ggtcggcaaa aacgttactt cggtttcaac agtaaaatgg cgtaatttgg cgggttggcg
    28321 acggggctgc ggtggctgcc agctactttg cgtcagcacc tgctccagac caaaccgaat
    28381 aacctcatgg cgatctgcgg acagcgggct gccatcgggt tccagcacga taaaggtatc
    28441 catcgccata ccgtcgcgag tggtgaaaat ttgtgcgtcg tgaacactta aattgcggcg
    28501 gtctaattcg gcacagacgg cggcaaacag ataggggcgg tccgggctcc agataaaaat
    28561 ctcggtgcct ccacgcgtag cctgcgggct aagcaatacc agcggtttgc ttaaatcatg
    28621 ctgtaataaa tggcgggcat gccaggccag ttgatttggg ctatggcgga caaaatagtt
    28681 agcacgacag cgtgaccaaa tttggtgcag cgcctcttcg tcgatgttat ccatgcgcag
    28741 tagtgccagt gcctggagtt ggtgatggcg aacccgttcg cgcatatccg gcgtgttttg
    28801 cattccgcgt cgtagctgct tttcggtggc aaaatagagt tcgcgcaaca gactttgctt
    28861 ccagctattc cacagtgttt cgttggtggc gcaaatgtca gccactgtca ggcataccag
    28921 atagcgcaga cgattttccg tttgaacttc ttcggcaaac tgcttgatga cttcagggtc
    28981 ctgaatatcg cggcgttgtg ctgtcaccga catcagcaag tgctggcgaa ccagccaggc
    29041 aaccagctgt gtttcgcgtg agttcagccc gtggagttct gcaaaatgca ctacatcctg
    29101 agcaccgaga atggagtggt cgccgccgcg tcctttggcg atatcgtgaa acagcgcggc
    29161 gatgaaaatc agctcagttg acggcaggcg cggccagacg tccacacaca acggatggcg
    29221 ctggcgcgtt tcttcactgg caaaactctc cagtttcagc atcacgcgga tagtatgttc
    29281 atccaccgtg taggcgtgga acagatcaaa ctgcatctgc ccgacgatat gcgaccattg
    29341 cggcatatac gcgccgagca cgctatggcg atgcattggc aatagtccgc gccgcaccgc
    29401 tccggggtga cgcagaatgc tcaaaaacag ttttcgtgct tgcggaatat tacacagcgg
    29461 ttgttgcaga tggcgacggg catggcgtaa ctggcgcagc gtggtggagt aaatgccggt
    29521 gatcgcactg ttgcgcacca tggtgtagaa catacgcaag atggcttccg gctggcgcat
    29581 aaatagtgtt tcatcacgca ggtcgattag cgtaccgcgt agctgaaact catcgtcgag
    29641 tggacgtggt ttttcgtcgg cgggaagggc gaggatggct tcatcgaaca gttgcagcag
    29701 catctggttg agttcactga cgcggcgtgt aacgcggaag taatccttca tcatccgctc
    29761 gaccggttcg ttgccttcac cactgtaatt cagacgctga gcgacgctaa gttggcgatc
    29821 gaataacaga cgattatcgt aacggctgac gaccagatgc agggcaaagc gaatacgcca
    29881 cagtatatgc agacattcgt ttaattccgc ccgctccgct gaggttaaga agccaaaccc
    29941 gaccatttca tccagcgatg ttgcgccaaa atgacggcgg gccacccatt gcagagtgtg
    30001 aatatcgcgc aagccgccag ggctactttt gatgtctggt tcaaggttgt agctggtgcc
    30061 atggtaacgc tgatggcgct ggttctgttc ttcaactttc gctgcgtaga acttgtcgga
    30121 aggccagaat ccttcgctaa aaatatgttt ttgcagttcg aggaagagcg caacatcgcc
    30181 aattaataag cgggattcga ttaaattggt ggcgacggtt aaatccgata acccttccaa
    30241 catgcactct tcaagcgtgc gcacactatg accgacttcc agctttacat cccagagcag
    30301 cgttaacagc tcgcccactt tttgcgcctg atcgtccggg agctttttac ggcttaaaat
    30361 cagtaaatcg atgtcagaaa gtggatgcag ctcgccacga ccgtagccac cgacggcaac
    30421 caatgccagg tcggcaatct ggctgaatcc cgcttcaatc cataatcgtt gcaggagctg
    30481 gtctatgaac tcggtacgtg cctcaatcaa ctgttctgca gagatcccat tgtcaaaggc
    30541 atcacccagc caacgctgga aagtatcgat atgggctttt atcccacaga cggttaattc
    30601 atcacggggc caggcgcatg gattttgcgg ttgaccgggc agggtggaga gagcggtgtt
    30661 tgcgtactgt tctggaaggg tattcattgt gcgccaccca taagattaaa ttatcgcatt
    30721 aaaaaagccg gcattcgccg gcttcatctt attcgtcgtg cgagattatc gccgggatgg
    30781 tgtcatcctt gcgtagcgtc aaaatttcgc agccgttatc agtcaccaca atagtatgct
    30841 catattgtgc agacaagctg cgatctttgg tttttaccgt ccagccatct ttcatggtgc
    30901 ggatctcttt tttacccgcg ttgaccattg gctcaatggt gaacgtcatc cccggtttca
    30961 gcacgacgtt ggtttcacgg gagtcatagt gcagcacctg cggttcttca tggaagccgc
    31021 gaccaatacc gtgtccgcaa tattcacgaa cgacggagaa gccttctgct tcgacaaatt
    31081 tctgaatcgc cgcaccgatt tcgcgcagat taatgcctgg ttttaccatg cgtagcgcca
    31141 ggtacaggct ttcttgcgtg atgcggcaca ggcgttcgcc catgatggtc ggcttaccga
    31201 cgataaacat tttcgaggta tcgccgtgga aaccatcttt gattacggtg acatcaatgt
    31261 taacgatatc gccatctttc agcagcttag catcgtccgg gataccgtgg cacaccactt
    31321 cattaataga gatgcaaacg gatttcggat agccgtgata gccgaggcag gcagaaaccg
    31381 cgtgttgttc attaacaatg taatcattac agatgcgatc cagctcgccg gtgctgacgc
    31441 ccggtttaac atacggttcg atcatctcca gcacttcggc agccagtcgg ccagcgacgc
    31501 gcattttttc gatatcttct ggggtcttga ttgagatagc cattaattct gtccatcagc
    31561 gtcggtgata ccgacaatat atatgtaagt gccgtcaatg gtatcacacc cgggcaaatt
    31621 gagaatcatt ctgaatttcg ccaaacgtgc cactgaaggt tttctataat agaaaattcg
    31681 acgtctgatg ctgtacacag cgccaacaat tattggtgtc cacgacgtat ttgtggtata
    31741 aagcgcgccg gacttccgat ccatttcgta tacacagact ggacggaagc gacaatctca
    31801 ctttgtgtaa caacacacac gtatcggcac atattccggg gtgccctttg gggtcggtaa
    31861 tatgggatac gtggaggcat aaccccaact tttatataga ggttttaatc atggcaactg
    31921 tttccatgcg cgacatgctc aaggctggtg ttcacttcgg tcaccagacc cgttactgga
    31981 acccgaaaat gaagccgttc atcttcggtg cgcgtaacaa agttcacatc atcaaccttg
    32041 agaaaactgt accgatgttc aacgaagctc tggctgaact gaacaagatt gtttctcgca
    32101 aaggtaaaat ccttttcgtt ggtactaaac gcgctgcaag cgaagcggtg aaagacgctg
    32161 ctctgagctg cgaccagttc ttcgtgaacc atcgctggct gggcggtatg ctgactaact
    32221 ggaaaaccgt tcgtcagtcc atcaaacgtc tgaaagacct ggaaactcag tctcaggacg
    32281 gtactttcga caagctgacc aagaaagaag cgctgatgcg cactcgtgag ctggagaaac
    32341 tggaaaacag cctgggcggt atcaaagaca tgggcggtct gccggacgct ctgtttgtaa
    32401 tcgatgctga ccacgaacac attgctatca aagaagcaaa caacttgggt attccggtat
    32461 ttgctatcgt tgataccaac tctgatccgg acggtgttga cttcgttatc ccgggtaacg
    32521 acgacgcaat ccgtgctgtg accctgtacc tgggcgctgt tgctgcaacc gtacgtgaag
    32581 gccgttctca ggatctggct tcccaggcgg aagaaagctt cgtagaagct gagtaataag
    32641 gcttgataac tcccccaaaa tagttcgagt tgcagaaagg cggcaagctc gagaattccc
    32701 gggagcttac atcagtaagt gaccgggatg agcgagcgaa gacaacgcat ctgcggcgcg
    32761 aaatatgaag ggggagagcc cttatagacc aggtagtaca cgtttggtta gggggcctgc
    32821 atatggcccc ctttttcact tttatatctg tgcggtttaa tgccgggcag atcacatctc
    32881 cgaggatttt agaatggctg aaattaccgc atccctggta aaagagctgc gtgagcgtac
    32941 tggcgcaggc atgatggatt gcaaaaaagc actgactgaa gctaacggcg acatcgagct
    33001 ggcaatcgaa aacatgcgta agtccggtgc tattaaagca gcgaaaaaag caggcaacgt
    33061 tgctgctgac ggcgtgatca aaaccaaaat cgacggcaac tacggcatca ttctggaagt
    33121 taactgccag actgacttcg ttgcaaaaga cgctggtttc caggcgttcg cagacaaagt
    33181 tctggacgca gctgttgctg gcaaaatcac tgacgttgaa gttctgaaag cacagttcga
    33241 agaagaacgt gttgcgctgg tagcgaaaat tggtgaaaac atcaacattc gccgcgttgc
    33301 tgcgctggaa ggcgacgttc tgggttctta tcagcacggt gcgcgtatcg gcgttctggt
    33361 tgctgctaaa ggcgctgacg aagagctggt taaacacatc gctatgcacg ttgctgcaag
    33421 caagccagaa ttcatcaaac cagaagacgt atccgctgaa gtggtagaaa aagaatacca
    33481 ggtacagctg gatatcgcga tgcagtctgg taagccgaaa gaaatcgcag agaaaatggt
    33541 tgaaggccgc atgaagaaat tcaccggcga agtttctctg accggtcagc cgttcgttat
    33601 ggaaccaagc aaaactgttg gtcagctgct gaaagagcat aacgctgaag tgactggctt
    33661 catccgtttc gaagtgggtg aaggcatcga gaaagttgag actgactttg cagcagaagt
    33721 tgctgcgatg tccaagcagt cttaattatc aaaaaggagc cgcctgaggg cggcttcttt
    33781 ttgtgcccat cttgtaaatt cagctaaccc ttgtggggct gcgctgaaaa gcgacgtaca
    33841 atgtcgctag tattaattca tttcaatcgt tgacagtctc aggaaagaaa catggctacc
    33901 aatgcaaaac ccgtctataa acgcattctg cttaagttga gtggcgaagc tctgcagggc
    33961 actgaaggct tcggtattga tgcaagcata ctggatcgta tggctcagga aatcaaagaa
    34021 ctggttgaac tgggtattca ggttggtgtg gtgattggtg ggggtaacct gttccgtggc
    34081 gctggtctgg cgaaagcggg tatgaaccgc gttgtgggcg accacatggg gatgctggcg
    34141 accgtaatga acggcttggc aatgcgtgat gcactgcacc gcgcctatgt gaacgctcgt
    34201 ctgatgtccg ctattccatt gaatggcgtg tgcgacagct acagctgggc agaagctatc
    34261 agcctgttgc gcaacaaccg tgtggtgatc ctctccgccg gtacaggtaa cccgttcttt
    34321 accaccgact cagcagcttg cctgcgtggt atcgaaattg aagccgatgt ggtgctgaaa
    34381 gcaaccaaag ttgacggcgt gtttaccgct gatccggcga aagatccaac cgcaaccatg
    34441 tacgagcaac tgacttacag cgaagtgctg gaaaaagagc tgaaagtcat ggacctggcg
    34501 gccttcacgc tggctcgtga ccataaatta ccgattcgtg ttttcaatat gaacaaaccg
    34561 ggtgcgctgc gccgtgtggt aatgggtgaa aaagaaggga ctttaatcac ggaataattc
    34621 ccgtgatgga taaataaggg taagattccg cgtaagtatc gcgggggcgt aagtctggtt
    34681 ataaggcgtt attgttgcag gcagtttggt cacggccagc gcgcagcaac cggagcgtac
    34741 aaaagtacgt gaggatggcg agcactgctc ggggccaaaa tggcaaataa aatagcctaa
    34801 taatccagac gattacccgt aatatgttta atcagggcta tacttagcac acttccactg
    34861 tgtgtgactg tctggtctga ctgagacaag ttttcaagga ttcgtaacgt gattagcgat
    34921 atcagaaaag atgctgaagt acgcatggac aaatgcgtag aagcgttcaa aacccaaatc
    34981 agcaaaatac gcacgggtcg tgcttctccc agcctgctgg atggcattgt cgtggaatat
    35041 tacggcacgc cgacgccgct gcgtcagctg gcaagcgtaa cggtagaaga ttcccgtact
    35101 ctgaaaatca acgtgtttga tcgttcaatg tctccggccg ttgaaaaagc gattatggcc
    35161 tccgatctcg gcctgaaccc gaactctgcg ggtagcgaca tccgtgttcc gctgccgccg
    35221 ctgacggaag aacgtcgtaa agatctgacc aaaatcgttc gtggtgaagc agaacaagcg
    35281 cgtgttgcag tacgtaatgt gcgtcgtgac gcgaacgaca aagtgaaagc actgttgaaa
    35341 gataaagaga tcagcgaaga cgacgatcgc cgttctcagg acgatgtaca gaaactgact
    35401 gatgctgcaa tcaagaaaat tgaagcggcg ctggcagaca aagaagcaga actgatgcag
    35461 ttctgatttc ttgaacgaca aaaacgccgc tcagtagatc cttgcggatc ggctggcggc
    35521 gttttgcttt ttattctgtc tcaactctgg atgtttcatg aagcaactca ccattctggg
    35581 ctcgaccggc tcgattggtt gcagcacgct ggacgtggtg cgccataatc ccgaacactt
    35641 ccgcgtagtt gcgctggtgg caggcaaaaa tgtcactcgc atggtagaac agtgcctgga
    35701 attctctccc cgctatgccg taatggacga tgaagcgagt gcgaaacttc ttaaaacgat
    35761 gctacagcaa caggggagcc gcaccgaagt cttaagtggg caacaagccg cttgcgatat
    35821 ggcagcgctt gaggaggttg atcaggtgat ggcagccatt gttggtgctg ctgggctgtt
    35881 acctacgctt gctgcgatcc gcgcgggtaa aaccattttg ctggccaata aagaatcact
    35941 ggttacctgc ggacgtctgt ttatggacgc cgtaaagcag agcaaagcgc aattgttacc
    36001 ggtcgatagc gaacataacg ctatttttca gagtttaccg caacctatcc agcataatct
    36061 gggatacgct gaccttgagc aaaatggcgt ggtgtccatt ttacttaccg ggtctggtgg
    36121 ccctttccgt gagacgccat tgcgcgattt ggcaacaatg acgcccgatc aagcttgccg
    36181 tcatccgaac tggtcgatgg gacgtaaaat ttctgtcgat tcggctacca tgatgaacaa
    36241 aggtctggaa tacattgaag cgcgttggct gtttaacgcc agcgccagcc agatggaagt
    36301 gctgattcac ccgcagtcag tgattcactc aatggtgcgc tatcaggacg gcagtgttct
    36361 ggcgcagctg ggggaaccgg atatgcgtac gccaattgcc cacaccatgg catggccgaa
    36421 tcgcgtgaac tctggcgtga agccgctcga tttttgcaaa ctaagtgcgt tgacatttgc
    36481 cgcaccggat tatgagcgtt atccatgcct gaaactggcg atggaggcgt tcgaacaagg
    36541 ccaggcagcg acgacagcat tgaatgccgc aaacgaaatc accgttgctg cttttcttgc
    36601 gcaacaaatc cgctttacgg atatcgctgc gttgaattta tccgtactgg aaaaaatgga
    36661 tatgcgcgaa ccacaatgtg tggacgatgt gttatctgtt gatgcgaacg cgagtgaagt
    36721 cgccagaaaa gaggtgatgc gtctcgcaag ctgaggataa tccggctaca gagagtcgcg
    36781 ctatttgtta gcgtagggct tcagtgatat agtctgcgcc atctgatcgt aagtagttgg
    36841 ctttataagg tcaggtatgc cgtggtttta cacggctttt ttttgtatag gcttcagtat
    36901 tcctgagtac cgtaaaccct gtcagggaat aaaaaacgcg tgatgttgtc tgctactcaa
    36961 ccacttagcg aaaaattgcc agcgcatggc tgccgtcatg ttgcgatcat tatggacggc
    37021 aatggtcgct gggcaaaaaa gcaagggaag attcgtgcct ttgggcataa agccggggca
    37081 aaatccgtcc gccgggctgt ctcttttgcg gccaacaacg gtattgaggc gttaacgctg
    37141 tatgccttta gtagtgaaaa ctggaaccga ccagcgcagg aagtcagtgc gttaatggaa
    37201 ctgtttgtgt gggcgctcga tagcgaagta aaaagtctgc accgacataa cgtgcgtctg
    37261 cgtattattg gcgataccag tcgttttaac tcgcgtttgc aagaacgtat tcgtaaatct
    37321 gaagcgctaa cagccggaaa taccggtctg acgctgaata ttgcggcgaa ctacggtgga
    37381 cgttgggata tagtccaggg agtcaggcaa ctggctgaaa aggtgcagca aggaaacctg
    37441 caaccagatc agatagatga agagatgtta aaccagcatg tctgtatgca tgaactggcc
    37501 cctgtagatt tagtaattag gactggggga gagcatcgca ttagtaactt tttgctttgg
    37561 caaattgcct atgccgaact ttactttaca gatgttctct ggcccgattt cgatgaacaa
    37621 gactttgaag gggcgttaaa tgcctttgct aatcgagagc gtcgtttcgg cggcaccgag
    37681 cccggtgatg aaacagcctg atgggggtcg cttttgctga agtatcgcct gatatctgct
    37741 tttgtgttaa tacccgtcgt catcgcggcg ttgtttctgt tgccgccggt ggggttcgcc
    37801 attgtaacgc tggtggtctg catgctggca gcgtgggaat ggggacagct tagcggtttt
    37861 accactcgtt cgcagcgagt atggttggcg gtgttatgcg ggttattgtt ggcgctgatg
    37921 ctttttctgt tgccggaata tcaccgaaat attcatcaac cgctggttga aatctcactt
    37981 tgggcttcgt tgggttggtg gattgtcgcg ctattgctgg tgctgtttta cccaggttcc
    38041 gcagcaatct ggcgtaactc taaaacattg cgccttattt ttggcgtgct aaccattgtt
    38101 cccttcttct ggggcatgct ggcgttacgg gcctggcact atgacgagaa tcattacagt
    38161 ggcgcaatat ggctgctcta tgtcatgatc ctggtatggg gcgctgactc cggcgcatat
    38221 atgtttggca aattgtttgg taaacataag ctggcaccga aggtttctcc gggtaaaacc
    38281 tggcaaggct ttataggtgg actcgctact gcagcggtaa tctcatgggg ttatggcatg
    38341 tgggcgaatc tcgacgtcgc tcccgtcacc ttactcattt gctctattgt cgcagcgtta
    38401 gcctcagtgc tcggcgatct gaccgagagt atgtttaagc gtgaagcagg aattaaggac
    38461 agcggtcatt taattccagg acacggtggt attttagatc gtattgatag cctgacggct
    38521 gcggtaccgg tctttgcttg cttgttgtta ctggtattca ggacgcttta acggaaggta
    38581 atatgctgag ttttctctgg gatttggctt cgttcatcgt tgcactgggt gtacttatca
    38641 ccgtgcatga atttggtcat ttctgggttg cccggcgttg tggtgttcgc gttgagcgtt
    38701 tctcaatagg gtttggtaag gcgctctggc ggcgaactga taagctcggc accgaatatg
    38761 ttatcgccct gatcccgttg ggcggttatg tcaaaatgct ggatgagcgc gcagaaccgg
    38821 tcgttccgga actccgccac catgccttca ataataaatc tgtcggccaa cgagcggcga
    38881 ttattgccgc aggtccggtt gcaaacttca tttttgctat ctttgcctac tggctggttt
    38941 ttattattgg tgtgcctggc gtacgtccgg tggttggcga aatagcagcc aattcgatag
    39001 ctgcggaagc acaaattgca ccaggtacgg aactaaaagc cgtagatggt atcgaaacgc
    39061 ctgattggga tgccgtgcgt ttgcagttgg tcgataaaat tggcgatgaa agcaccacca
    39121 ttacagtagc gccatttgac agcgaccaac ggcgggatgt aaagctcgat ttacgtcact
    39181 gggcgtttga gcctgataaa gaagatccgg tatcttcgct ggggattcgt cctcgtgggc
    39241 cgcaaattga acctgtactg gaaaatgtgc agccaaactc ggcggcaagc aaggcaggtt
    39301 tgcaagcagg cgacaggatc gttaaagtcg atggtcagcc cttaacgcag tgggtgacct
    39361 ttgtgatgct tgtccgggat aacccgggta aatccttagc gttagaaatc gaaaggcagg
    39421 ggagtccctt gtctttgaca ttaatcccgg agagtaaacc gggtaatggt aaagcgattg
    39481 gttttgtcgg tattgagccg aaagtcattc ctttgccaga tgagtataaa gttgtacgcc
    39541 agtatgggcc gttcaacgcc atcgtcgaag ccacggacaa aacgtggcag ctgatgaagc
    39601 tgacggtcag tatgctggga aaattgatca ccggtgatgt gaaactgaac aacctcagtg
    39661 ggccgatctc tatcgccaag ggggctggga tgacagcgga actcggggta gtttattacc
    39721 tgccgtttct tgcgcttatt agcgtgaact tagggataat taacctgttt ccgttgcccg
    39781 tacttgacgg ggggcatctg ctgttccttg cgatcgaaaa gatcaagggc ggaccggtat
    39841 ccgagcgggt tcaagacttt tgttatcgca ttggctcgat tctgctggtg ctgttaatgg
    39901 ggcttgcact tttcaatgat ttctctcggt tatgagagtt agttaggaag aacgcataat
    39961 aacgatggcg atgaaaaagt tgctcatagc gtcgctgctg tttagcagcg ccaccgtata
    40021 cggtgctgaa gggttcgtag tgaaagatat tcatttcgaa ggccttcagc gtgtcgccgt
    40081 tggtgcggcc ctcctcagta tgccggtgcg tacaggcgac acggttaatg atgaagatat
    40141 cagtaatacc attcgcgctc tgtttgctac cggcaacttt gaggatgttc gcgtccttcg
    40201 tgatggtgat acccttctgg ttcaggtaaa agaacgtccg accattgcca gcattacttt
    40261 ctccggtaac aaatcggtga aagatgacat gctgaagcaa aacctcgagg cttctggtgt
    40321 gcgtgtgggc gaatccctcg atcgcaccac cattgccgat atcgagaaag gtctggaaga
    40381 cttctactac agcgtcggta aatatagcgc cagcgttaaa gctgtcgtga ccccgctgcc
    40441 gcgcaaccgt gttgacctaa aactggtgtt ccaggaaggt gtgtcagctg aaatccagca
    40501 aattaacatt gttggtaacc atgctttcac caccgacgaa ctgatctctc atttccaact
    40561 gcgtgacgaa gtgccgtggt ggaatgtggt aggcgatcgt aaataccaga aacagaaact
    40621 ggcgggcgat cttgaaaccc tgcgcagcta ctatctggac cgcggttatg cccgtttcaa
    40681 catcgactct acccaggtca gtctgacgcc agataaaaaa ggtatttacg tcacggtgaa
    40741 catcaccgaa ggcgatcagt acaagctttc tggcgttgaa gtgagcggca accttgccgg
    40801 gcactccgct gaaattgagc aactgactaa gatcgagccg ggcgagctgt ataacggcac
    40861 taaagtgacc aaaatggaag atgacattaa aaagcttctc ggtcgctatg gttatgccta
    40921 tccgcgcgta cagtcgatgc ctgaaattaa cgatgccgac aaaaccgtta aattacgcgt
    40981 gaacgttgat gcgggtaacc gtttctacgt gcgtaagatc cgttttgaag gtaacgatac
    41041 ctcgaaagat gccgtcctgc gtcgcgaaat gcgtcagatg gaaggtgcat ggttggggag
    41101 cgatctggtc gatcagggta aggagcgtct gaatcgtctg ggcttctttg aaactgtcga
    41161 taccgatacc caacgtgttc cgggtagccc ggaccaggtt gatgtagtct acaaggtgaa
    41221 agaacgtaac actggtagct tcaacttcgg gattggttac ggtactgaaa gtggcgtgag
    41281 cttccaggct ggcgtgcaac aggataactg gttaggtaca ggttatgctg ttggtatcaa
    41341 cgggaccaaa aacgattacc agacctatgc tgaactgtcg gtaaccaacc cgtacttcac
    41401 cgtagatggc gtaagcctcg gtggtcgtct cttctataat gacttccagg cagatgacgc
    41461 cgacctgtcc gactatacca acaagagtta tggtacagac gtgacgttgg gcttcccgat
    41521 taacgaatat aactcgctgc gtgcaggtct gggttatgta cataactccc tgtccaacat
    41581 gcagcctcag gttgcgatgt ggcgttatct gtactctatg ggtgaacatc cgagcacctc
    41641 tgatcaggat aacagcttca aaacggacga cttcacgttc aactatggtt ggacctataa
    41701 caagcttgac cgtggttact tcccgacaga tggttcacgt gtcaacctga ccggtaaagt
    41761 gaccattcct ggatcggata acgaatacta caaagtgacg ttagacacgg cgacttatgt
    41821 gccgatcgat gacgatcaca aatgggttgt tctgggtcgt acccgctggg gttatggtga
    41881 tggtttaggc ggcaaagaga tgccgttcta cgagaacttc tatgccggtg gttccagcac
    41941 cgtgcgtggc ttccagtcca ataccattgg tccgaaagca gtttacttcc cgcatcaggc
    42001 cagtaattat gatccggact atgattacga atgtgcgact caggacggcg cgaaagacct
    42061 gtgtaaatcg gatgatgctg taggcggtaa cgccatggcg gttgccagcc tcgagttcat
    42121 caccccgacg ccgtttatta gcgataagta tgctaactcg gttcgtactt cattcttctg
    42181 ggatatgggt accgtttggg atacaaactg ggattccagc caatattctg gatatccgga
    42241 ctatagtgat ccaagcaata tccgtatgtc tgcgggtatc gcattacaat ggatgtcccc
    42301 attggggccg ttggtgttct cctacgccca gccgttcaaa aagtacgatg gagacaaggc
    42361 agaacagttc cagtttaaca tcggtaaaac ctggtaagtg ttctccacaa aggaatgtag
    42421 tggtagtgta gcgatgactt taggcgatca atataagatc gccgggccac gcaaagaact
    42481 gcaccctccg gtgcaaatgg gatggtaagg agtttattgt gaaaaagtgg ttattagctg
    42541 caggtcttgg tttagcactg gcaacttctg ctcaggcggc tgacaaaatt gcaatcgtca
    42601 atatgggcag cctgttccag caggtagcgc agaaaaccgg tgtttctaac acgctggaaa
    42661 atgagttcaa aggccgtgcc agcgaactgc agcgtatgga aaccgatctg caggctaaaa
    42721 tgaaaaaact gcagtccatg aaagcgggca gcgatcgcac caaactggaa aaagacgtga
    42781 tggctcagcg ccagactttt gctcagaaag cgcaggcttt tgagcaggat cgcgcacgtc
    42841 gttccaacga agaacgcggc aaactggtta ctcgtatcca gactgctgtg aaatccgttg
    42901 ccaacagcca ggatatcgat ctggttgttg atgcaaacgc cgttgcttac aacagcagcg
    42961 atgtaaaaga catcactgcc gacgtactga aacaggttaa ataagtaatg ccttcaattc
    43021 gactggctga tttagcgcag cagttggatg cagaactaca cggtgatggc gatatcgtca
    43081 tcaccggcgt tgcgtccatg caatctgcac aaacaggtca cattacgttc atggttaacc
    43141 caaaataccg tgagcattta ggcttgtgcc aggcgtccgc ggttgtcatg acccaggacg
    43201 atcttccttt cgcgaaaagt gccgcgctgg tagtgaagaa tccctacctg acttacgcgc
    43261 gcatggcgca aattttagat accacgccgc agcccgcgca gaacattgca cccagtgcgg
    43321 tgatcgacgc gacggcgaag ctgggtaaca acgtatcgat tggcgctaac gcggtgattg
    43381 agtccggcgt tgaactgggc gataacgtga ttatcggtgc cggttgcttc gtaggtaaaa
    43441 acagcaaaat cggtgcaggt tcgcgtctct gggcgaacgt aaccatttac catgagatcc
    43501 agatcggtca gaattgcctg atccagtccg gaacagtggt aggcgcagac ggctttggtt
    43561 atgccaacga tcgtggtaac tgggtgaaga tcccacagat tggtcgcgta attattggcg
    43621 atcgcgtgga gatcggtgcc tgcacaacca tcgatcgcgg cgcgctggat gacactgtta
    43681 ttggcaatgg cgtgatcatt gataaccagt gccagattgc acataacgtc gtgattggcg
    43741 acaatacggc ggttgccggt ggcgtcatta tggcgggcag cctgaaaatt ggtcgttact
    43801 gcatgatcgg cggagccagc gtaatcaacg ggcatatgga aatatgcgac aaagtgacgg
    43861 ttacgggcat gggtatggtg atgcgtccca tcactgaacc aggcgtctat tcctcaggca
    43921 ttccgctgca acccaacaaa gtctggcgca aaaccgctgc actggtgatg aacattgatg
    43981 acatgagcaa gcgtctgaaa tcgcttgagc gcaaggttaa tcaacaagac taacgttcca
    44041 tcttttgttc gccaaacttt acggcctgtc tcattcttac gattgcggca ggccgtgtta
    44101 ttattgtcgt ttcttatatt ttgacaggaa gagtatcttg actactaaca ctcatactct
    44161 gcagattgaa gagattttag aacttctgcc gcaccgtttc ccgttcttac tggtggatcg
    44221 cgtgctggat tttgaagaag gtcgttttct gcgcgcagta aaaaatgtct ctgtcaatga
    44281 gccattcttc cagggccatt tccctggaaa accgattttc ccgggtgtgc tgattctgga
    44341 agcaatggca caggcaacag gtattctggc gtttaaaagc gtaggaaaac tggaaccggg
    44401 tgagctgtac tacttcgctg gtattgacga agcgcgcttc aagcgcccgg tcgtgcctgg
    44461 cgatcaaatg atcatggaag tcactttcga aaaaacgcgc cgcggcctga cccgttttaa
    44521 aggggttgct ctggtcgatg gtaaagtagt ttgcgaagca acgatgatgt gtgctcgtag
    44581 ccgggaggcc tgatacgtga ttgataaatc cgcctttgtg catccaaccg ccattgtgga
    44641 agagggcgcg tcaattggcg cgaacgcaca cattggtcct ttttgtatcg ttggacccca
    44701 tgtcgaaatt ggtgagggta ccgtactgaa atctcacgtt gtcgtgaatg gtcatactaa
    44761 aattggccgc gataatgaga tttatcagtt tgcctccatc ggcgaagtta accaggattt
    44821 gaaatatgct ggcgaaccga cccgtgtgga aatcggcgat cgtaaccgca ttcgcgaaag
    44881 cgtcaccatt catcgtggca cagtccaggg cggtggattg acgaaggtgg gcagcgacaa
    44941 cttactgatg atcaacgcgc acattgcgca cgattgtacg gtaggtaacc gctgtattct
    45001 cgccaacaac gcaacgctgg cgggtcacgt atcggttgac gacttcgcga tcatcggcgg
    45061 catgaccgca gtccatcagt tctgcatcat tggtgcgcat gtgatggttg gcggctgctc
    45121 cggtgtggcg caggacgtcc ctccttatgt tattgcgcag ggtaaccacg caacgccgtt
    45181 cggtgtcaat atcgaagggc tgaagcgccg cggattcagc cgtgaggcga ttaccgctat
    45241 ccgcaatgcg tataagctaa tttatcgtag cggtaaaacg ctcgatgaag tgaaaccgga
    45301 aattgctgaa ctggcggaaa catatccgga agtgaaagcc tttaccgatt tctttgcacg
    45361 ctcaacgcgc ggtctgattc gttaatgact gaccagcgtc cattaacgat tgccctggtc
    45421 gccggagaaa cctccggcga tatcctgggg gccggtttaa tccgcgctct gaaagaacgt
    45481 gtgcccaacg cccgctttgt tggtgttgcc gggccacgaa tgcaggctga aggctgcgaa
    45541 gcctggtacg aaatggaaga actggcggtg atgggcattg ttgaagtgct cggtcgtctg
    45601 cgtcgcttac tgcatattcg tgccgatctg acaaagcgtt ttggcgaact gaagccagat
    45661 gtttttgttg gtattgatgc gcccgacttc aatattactc ttgaaggtaa cctcaaaaag
    45721 cagggtatca aaaccattca ttacgtcagt ccatccgtct gggcgtggcg acagaaacgc
    45781 gttttcaaaa taggcagagc caccgatctg gtgctcgcat ttctgccttt cgaaaaagcg
    45841 ttttatgaca aatacaatgt accgtgccgc tttatcggtc ataccatggc tgatgccatg
    45901 ccgttagatc cagataaaaa tggtgcccgt gatgtgctgg ggatccctta cgatgcccac
    45961 tgtctggcat tgttgccggg cagccgtggt gcagaagtcg aaatgcttag tgccgatttc
    46021 ttgaaaactg cccagctttt gcgccagaca tatccggatc tcgaaatcgt ggtgccgctg
    46081 gtgaatgcca aacgccgcga gcagtttgaa cgcatcaaag ctgaagtcgc gccagacctg
    46141 tcggttcatt tgctggatgg aatgggccgt gaggcgatgg tcgccagcga tgcggcacta
    46201 ctggcgtcgg ggacggcagc cctggagtgt atgctggcga aatgcccgat ggtggtggga
    46261 tatcgcatga agccttttac cttctggttg gcgaagcggc tggtgaaaac tgattatgtc
    46321 tcgctgccaa atctgctggc gggcagagag ttagtcaaag agttattgca ggaagagtgt
    46381 gagccgcaaa aactggctgc ggcgctgtta ccgctgttgg cgaacgggaa aaccagccac
    46441 gcgatgcacg ataccttccg tgaactgcat cagcagatcc gctgcaatgc cgatgagcag
    46501 gcggcacaag ccgttctgga gttagcacaa tgatcgaatt tgtttatccg cacacgcagc
    46561 tggttgcggg tgtggatgaa gtcggacgcg ggccgttagt tggcgcggtc gtcaccgctg
    46621 cggtgatcct tgacccggcg cgcccgattg ccgggctgaa tgattccaaa aagctgagcg
    46681 aaaaacgccg tctggcgctc tatgaagaga tcaaagagaa agcgttgagc tggagtctgg
    46741 gccgcgcgga accccacgaa atcgacgagc tgaacattct tcatgcgacc atgctggcga
    46801 tgcagcgtgc cgtcgctggg ctgcatattg cgccggaata tgtgttgatt gatggtaacc
    46861 gctgcccgaa attaccgatg cctgcgatgg ctgtggtgaa aggggatagc cgcgtaccag
    46921 aaatcagtgc cgcgtctatc ctggcgaaag tgacgcgtga cgccgaaatg gcggcgctgg
    46981 atattgtttt cccgcaatat ggttttgccc aacataaagg gtatccaacc gcttttcatc
    47041 tggaaaaact ggctgaatat ggcgcgactg aacatcatcg tcgcagcttt gggcctgtca
    47101 aacgcgcact gggacttgcg tcctgattct tgtgtcgaga ttaagtaaac cggaatctga
    47161 agatgtctga accacgtttc gtacacctgc gggtgcacag cgactactcg atgatcgatg
    47221 gcttggccaa aaccgcaccg ctggtaaaaa aggcggcggc gttgggtatg ccagcactgg
    47281 cgatcaccga tttcaccaac ctttgcggtc tggtgaagtt ctacggagcg ggacatggcg
    47341 cagggattaa gcctatcgtc ggggcagatt ttaatgtcca gtgcgacctg ctgggtgatg
    47401 agttaaccca cctgacggta ctggcggcga acaataccgg ctatcagaat ctggcgttgc
    47461 tgatctcaaa agcgtatcag cgcgggtacg gtgccgccgg gccgatcatc gatcgcgact
    47521 ggcttatcga attaaacgaa gggttgatcc ttctttccgg cgggcgcatg ggcgacgtcg
    47581 gacgcagtct tttgcgtggt aacagcgcgc tggtagatga gtgtgtcgcg ttttatgaag
    47641 aacacttccc ggatcgctat tttctcgagc tgatccgcac cggcaggccg gatgaagaaa
    47701 gctatctgca cgcggcggtg gaactggcgg aagcgcgcgg tttgcccgtc gtggcgacta
    47761 acgatgtgcg ctttatcgac agcagcgact ttgacgcaca cgaaatccgc gtcgcgatcc
    47821 acgacggctt taccctcgac gatcctaaac gcccgcgtaa ctattcgccg cagcaatata
    47881 tgcgtagcga agaggagatg tgtgagctgt ttgccgacat cccagaagcc cttgccaaca
    47941 ccgttgagat cgccaaacgc tgtaacgtaa ctgtgcgtct tggtgaatac ttcctgccgc
    48001 agttcccgac cggggacatg agcaccgaag attatctggt caagcgtgca aaagaaggcc
    48061 tggaagagcg tctggcattt ttattccctg acgaggaaga acgtcttaaa cgccgcccgg
    48121 aatatgacga acgtctggag actgaacttc aggttatcaa ccagatgggc ttcccgggct
    48181 acttcctcat cgttatggaa tttatccagt ggtcgaaaga taacggcgta ccggtagggc
    48241 caggccgtgg ctccggtgcg ggttcactgg tggcctacgc gctgaaaatc accgacctcg
    48301 atccgctgga atttgacctg ctgttcgaac gtttccttaa cccggaacgt gtctccatgc
    48361 ctgacttcga cgttgacttc tgtatggaga aacgcgatca ggttatcgaa cacgtggcgg
    48421 acatgtacgg tcgtgatgcg gtatcgcaga ttatcacctt cggtacgatg gcggcgaaag
    48481 cggtgatccg cgacgtaggc cgcgtgctgg ggcatccgta cggctttgtc gaccgtatct
    48541 cgaaactgat cccgcccgat ccggggatga cgctggcgaa agcgtttgaa gccgagccgc
    48601 agctgccgga aatctacgaa gcggatgaag aagttaaggc gctgatcgac atggcgcgca
    48661 aactggaagg ggtcacccgt aacgccggta agcacgccgg tggggtggtt atcgcgccga
    48721 ccaaaattac cgattttgcg ccgctttatt gcgatgaaga gggcaaacat ccggtcaccc
    48781 agtttgataa aagcgacgtt gaatacgccg gactggtgaa gttcgacttc cttggtttgc
    48841 gtacgctcac catcatcaac tgggcgctgg agatgatcaa caagcggcgg gcgaagaatg
    48901 gcgagccacc gctggatatc gccgcgatcc cgctggatga caagaaaagc ttcgacatgc
    48961 tgcaacgctc ggaaaccacg gcggtattcc agcttgaatc gcgcggcatg aaggacctga
    49021 tcaagcgtct gcaacctgac tgcttcgaag atatgatcgc actggtggca ctgttccgcc
    49081 ctggtccgtt gcagtcaggg atggtggata actttatcga ccgtaaacat ggtcgtgaag
    49141 agatctccta tccggacgtg cagtggcagc atgaaagcct gaaatcggta ctggagccaa
    49201 cctacggcat tatcttgtat caggaacagg tcatgcagat tgcccaggtg ctttctggtt
    49261 ataccctcgg tggcgcggat atgctgcgcc gtgcgatggg taagaaaaag ccggaagaga
    49321 tggctaagca gcgttctgta tttgctgaag gtgcagaaaa gaacggaatc aacgccgaac
    49381 tggcgatgaa aatcttcgac ctggtggaga aattcgcggg ttacggattt aacaaatcgc
    49441 actctgcggc ctatgctttg gtgtcatatc aaacgttatg gctgaaagcg cactatccgg
    49501 cggagtttat ggcggcggta atgaccgccg atatggacaa caccgagaag gtggtgggcc
    49561 tggtggatga gtgctggcgg atggggctga aaatcctgcc accagatata aactccggtc
    49621 tttaccattt ccacgtcaac gacgatggcg aaatcgtgta tggtattggc gccatcaaag
    49681 gggtaggtga aggtccgatt gaggccatca tcgaagcccg taataaaggc ggctacttcc
    49741 gcgaactgtt tgatctctgc gcccgtaccg acaccaaaaa gttaaaccgg cgggtgctgg
    49801 aaaaactgat catgtccggg gcgtttgacc gtcttgggcc acaccgcgcg gcgctgatga
    49861 actcgctggg tgatgcgtta aaagcggcag atcaacacgc gaaagcggaa gctatcggtc
    49921 aggccgatat gttcggcgtg ctggccgaag agccggaaca aattgaacaa tcctacgcca
    49981 gctgccaacc gtggccggag caggtggtat tagatgggga acgtgaaacg ttaggcctgt
    50041 acctgaccgg acaccctatc aaccagtatt taaaagagat tgagcgttat gtcggaggcg
    50101 taaggctgaa agacatgcac ccgacagaac gtggtaaagt catcacggct gcggggctcg
    50161 ttgttgccgc gcgggttatg gtcaccaagc gcggcaatcg tatcggtatc tgcacgctgg
    50221 atgaccgttc cgggcggctg gaagtgatgt tgtttactga cgccctggat aaataccagc
    50281 aattgctgga aaaagaccgc atacttatcg tcagcggaca ggtcagcttt gatgacttca
    50341 gcggtgggct taaaatgacc gctcgcgaag tgatggatat tgacgaagcc cgggaaaaat
    50401 atgctcgcgg gcttgctatc tcgctgacgg acaggcaaat tgatgaccag cttttaaacc
    50461 gactccgtca gtctctggaa ccccaccgct ctgggacaat tccagtacat ctctactatc
    50521 agagggcgga tgcacgcgcg cggttgcgtt ttggcgcgac gtggcgtgtc tctccgagcg
    50581 atcgtttatt aaacgatctc cgtggcctca ttggttcgga gcaggtggaa ctggagtttg
    50641 actaatacag gaatactatg agtctgaatt tccttgattt tgaacagccg attgcagagc
    50701 tggaagcgaa aatcgattct ctgactgcgg ttagccgtca ggatgagaaa ctggatatta
    50761 acatcgatga agaagtgcat cgtctgcgtg aaaaaagcgt agaactgacg cgtaaaatct
    50821 tcgccgatct cggtgcatgg cagattgcgc aactggcacg ccatccgcag cgtccttata
    50881 ccctggatta cgttcgcctg gcatttgatg aatttgacga actggctggc gaccgtgcgt
    50941 atgcagacga taaagctatc gtcggtggta tcgcccgtct cgatggtcgt ccggtgatga
    51001 tcattggtca tcaaaaaggt cgtgaaacca aagaaaaaat tcgccgtaac tttggtatgc
    51061 cagcgccaga aggttaccgc aaagcactgc gtctgatgca aatggctgaa cgctttaaga
    51121 tgcctatcat cacctttatc gacaccccgg gggcttatcc tggcgtgggc gcagaagagc
    51181 gtggtcagtc tgaagccatt gcacgcaacc tgcgtgaaat gtctcgcctt ggcgtaccgg
    51241 tagtttgtac ggttatcggt gaaggtggtt ctggcggtgc gctggcgatt ggcgtgggcg
    51301 ataaagtgaa tatgctgcaa tacagcacct attccgttat ctcgccggaa ggttgtgcgt
    51361 ccattctgtg gaagagcgcc gacaaagcgc cgctggcggc tgaagcgatg ggtatcatcg
    51421 ctccgcgtct gaaagagctg aaactgatcg actccatcat cccggaaccg ctgggcggcg
    51481 ctcaccgtaa cccggaagcg atggcggcat cgttgaaagc gcaactgctg gcggatctgg
    51541 ccgatctcga tgtgttaagc actgaagatt taaaaaatcg tcgttatcag cgcctgatga
    51601 gctacggtta cgcgtaattc gcaaaagttc tgaaaaaggg tcacttcggt ggcccttttt
    51661 tatcgccacg gtttgagcag gctatgatta aggaaggatt ttccaggagg aacacatgaa
    51721 catcattgcc attatgggac cgcatggcgt cttttataaa gatgagccca tcaaagaact
    51781 ggagtcggcg ctggtggcgc aaggctttca gattatctgg ccacaaaaca gcgttgattt
    51841 gctgaaattt atcgaacata accctcgaat ttgcggcgtg atttttgact gggatgagta
    51901 cagtctcgat ttatgcagcg atatcaatca acttaatgaa tatctcccgc tttatgcctt
    51961 catcaacacc cactcgacga tggatgtcag cgtgcaggat atgcggatgg cgctctggtt
    52021 ttttgaatat gcgctggggc aggcggaaga tatcgccatt cgtatgcgtc agtacaccaa
    52081 cgaatatctt gataacatta cgccgccgtt cacgaaagcc ttgtttacct acgtcaaaga
    52141 gcggaagtac accttttgta cgccggggca tatgggcggc accgcatatc aaaaaagccc
    52201 ggttggctgt ctgttttatg attttttcgg cgggaatacc cttaaggctg atgtctctat
    52261 ttcggtcacc gagcttggtt cgttgcttga ccacaccggg ccacacctgg aagcggaaga
    52321 gtacatcgcg cggacttttg gcgcggaaca gagttatatc gttaccaacg gaatatcgac
    52381 gtcgaacaaa attgtgggta tgtacgccgc gccatccggc agtacgctgt tgatcgaccg
    52441 caactgccat aaatcactgg cgcatctgtt gatgatgaac gatgtagtgc cagtctggct
    52501 gaaaccgacg cgtaatgcgt tggggattct gggggggatc ccgcgccgtg aatttactcg
    52561 cgacagcatc gaagagaaag tcgctgccac tacgcaagca caatggccgg ttcatgcggt
    52621 gatcaccaac tccacctatg atggtttgct ctacaacacc gactggatca aacagacgct
    52681 ggatgtcccg tcgatccact tcgattccgc ctgggtgccg tatactcatt ttcatccaat
    52741 ctaccagggt aaaagtggta tgagcggcga gcgtgttgcg ggaaaagttt tcttcgaaac
    52801 gcaatcgacc cacaaaatgc tggcggcgtt atcgcaggct tcactgatcc acattaaagg
    52861 cgagtatgac gaagaggcgt ttaacgaagc ctttatgatg cataccacca cctcgcccag
    52921 ttatcccatt gttgcttcgg ttaagacggc ggcggcgatg ctgcgtggta atccgggcaa
    52981 acggctgatt aaccgttcgg tggagcgggc gctgcatttt cgcaaagagg tccagcggct
    53041 gcgggaagag tctgacggtt ggtttttcga tatctggcaa ccgccgcagg tggatgaagc
    53101 cgaatgcagg cccgttgcgc ctggcgaaca atggcacggc tttaacgatg cggatgccga
    53161 tcatatgttt ctcgatccgg ttaaagtcac tattttgaca ccggggatgg acgaacaggg
    53221 caatatgagt gaggagggga tcccggcggc gctggtagca aaattcctcg acgaacgtgg
    53281 gatcgtagta gagaaaaccg gcccttataa cctgctgttt ctctttagta ttggcatcga
    53341 taaaaccaaa gcaatgggat tattgcgtgg gttgacggaa tttaagcgct cttacgatct
    53401 caacctgcgg atcaaaaata tgctgcccga tctctatgct gaagatcccg atttctaccg
    53461 caatatgcgt attcaggatc tggcgcaagg gatccataag ctgattcgta aacacgatct
    53521 ttccggtttg atgttgcggg cattcgatac tttgccggaa atgatcatga cgccacatca
    53581 ggcatggcag agacagatta aaggtgaagt agaaaccatt gcgctggaac aactggtcgg
    53641 tagagtatcg gcaaatatga tcctgcctta tccaccgggc gtaccgctgt tgatgccagg
    53701 agaaatgctg accaaagaga gccgcacggt actcgatttt ctactgatgc tttgttccgt
    53761 agggcaacat taccccggtt ttgaaacgga tattcacggc gcgaaacagg acgaagacgg
    53821 cgtttaccgc gtacgagtcc taaaaatggc gggataactt gccagagcgg cttccgggcg
    53881 agtaacgtgc tgttaacaaa taaaggagac gttatgctgg gtttaaaaca ggttcaccat
    53941 attgcgatta ttgcgacgga ttatgcggtg agcaaagctt tctactgcga tattcttggt
    54001 ttcacgctgc aaagcgaagt ctatcgcgaa gcgcgcgact cgtggaaagg ggatttggcg
    54061 cttaatgggc aatatgtgat tgagcttttc tcttttccgt tcccgccgga gcgccccagc
    54121 cgaccggaag cttgcggcct gcgtcatctg gcttttagcg ttgatgacat cgatgcggca
    54181 gtggcgcacc ttgaaagcca taacgtgaag tgtgaagcca tccgtgtcga tccatacacg
    54241 caaaaacgct tcaccttctt taacgatccg gacgggctgc cgttggaact gtatgagcag
    54301 taaggcttgt catcgccgca tttgcccggt aacgtgccgg gcattgctac tgtaaaatcg
    54361 caccatcatg acactcacgc tcaatagaca acttctcacc tcacgccaga ttctggtggc
    54421 ctttagcggc gggcttgact ccaccgttct gctgcatcag ttggtgcagt ggcggacgga
    54481 aaatccgggc gtcactctgc gcgctattca tgtgcatcac ggtttaagtg ccaatgccga
    54541 tgcctgggtt acgcattgcg aaaacgtctg ccaacagtgg caggtgccgc tggtggtcga
    54601 acgcgtacaa cttgcgcaag aggggctggg gattgaggcc caggcgcggc aggcacgtta
    54661 tcaggcattt gcccgtaccc tattgcccgg cgaagtgctg gtcaccgcgc aacatctcga
    54721 cgatcaatgt gaaacctttc tgctggcgct aaaacgcggt agcggccctg ccgggctttc
    54781 ggctatggcg gaagtttcgg agtttgctgg aacacggctt atccgcccgt tgcttgcccg
    54841 cacgcggggg gaactggagc agtgggcatt ggcgcatggt ttacgctgga ttgaagacga
    54901 aagtaatcag gacgactcat acgatcgtaa ctttctgcgc ctgcgcgtag tgccgttatt
    54961 gcagcagcgt tggccgcatt ttgcggaagc aacggcccgc agcgccacac tttgtgctga
    55021 acaagagagt ctgttggatg aactgctggc ggatgattta gcacactgcc aaacgtcgca
    55081 ggggacgctg cagattgcgc caatgctggc gatgagtgat gcccgtcgcg cggcgattat
    55141 tcgccgctgg ctggcagggc agaatgcacc gatgccttcc cgcgacgcgc tggtgcgcat
    55201 ctggcaggaa gtggcgctgg cgcgggaaga tgcttccccc tgtttacgtt tgggcgcctt
    55261 tgaaatccgc cgctatcagt cgcaactgtg gtggattaaa tccgtcaccg ggcaaagcga
    55321 aaccattgtg ctgtggcaga cgtggctaca accgctggaa ttaccggcgg ggctgggaac
    55381 cgtacagctt actgcgggag gcgatattcg ccctccgcgt gcagacgaag cggtcagcgt
    55441 gcgtttcaaa gcgccaggat tgctgcatat tgtcgggcgc aacggcggac gtaagctgaa
    55501 gaaaatctgg caagagttag gcgtgccgcc gtggctacgt gacaccacgc cactgctgtt
    55561 ttatggagaa acgctgattg cggcggcagg ggtatttgtg acgcaagaag gtgtggctga
    55621 aggtgagaat ggcgtaagtt ttgtctggca gaaaacgctt agttaagtga aagccggata
    55681 agacgcatca aacgtcgcat ccggcgaaag tcaatcagga ttcgcttacc accaccgtac
    55741 cgatttcagg gtggctaaag ctggtaattt tatccagacg cagctcgcgg gtttcgccag
    55801 cggcctcgac gaccagatac tccacatttt tgcgggagac taaatcactg gcttttgcct
    55861 gcaatttttc gccatctttc agctcaagtg tcagcattaa atgatgctgg caggcgagct
    55921 caagattatc gtaatcatca caattaattg gttgatacgt atcattcatt gacataatcg
    55981 ctcaccagta agtttgccgc agcgtatgct gctttttccc tgacagcctc agaaagggcg
    56041 tcgtcggcag ccatttcatt cagcactttc aaaacgcagc ccagcgcgtc cggaacgtat
    56101 cctaagtctc cgctggcgat ttccgcgtac cgcttgcgta ttaactcaca atatttttcc
    56161 acatgccctc ctgtcagcac tctgacttaa ccgtggatgc aagtctaagc ctacgaagat
    56221 aaactctgtt tcgcaaggtg actataccac actcatttct gcaatatcag cgccgcaact
    56281 gcacgtattc cgttacaatg gcctcctgat tcgaaaggag ttttcttatg gcgcttaaag
    56341 cgacaattta taaagcgacg gttaatgtgg ccgatctcga ccgcaaccag tttctcgatg
    56401 cctctctgac gctggcgcgc cacccttcag aaacccagga gcgtatgatg ctgcgcttgc
    56461 tggcgtggct gaaatatgct gatgaacgtc tgcaatttac ccgtggtttg tgtgccgatg
    56521 atgagccgga agcgtggctg cgtaacgatc acctgggcat tgatttgtgg attgagctgg
    56581 ggctgccgga tgagcgacga attaagaaag cctgcaccca ggccgcagaa gtggcgctgt
    56641 ttacctataa tagtcgggcg gcgcaaatct ggtggcagca aaatcagagc aaatgtgtgc
    56701 agtttgccaa tctttccgtc tggtatctgg acgatgaaca actggcgaaa gtaagcgcct
    56761 ttgccgatcg taccatgacg ctgcaggcaa cgattcagga tggtgttatc tggttatcgg
    56821 atgataagaa taatctggaa gttaacttaa ccgcctggca acaaccttca tgattgtgat
    56881 ttcccgacat gttgctattc ccgatggtga gcttgagatc accgccattc gtgcgcaggg
    56941 cgctggcggg cagcatgtta ataagacctc aacggctatt catctgcgtt ttgacattcg
    57001 ggcgtccagc ctgccagagt attacaaaga gcgtctgctc gccgccagcc atcatttgat
    57061 cagcagtgat ggcgtgattg tcattaaggc acaggaatac cgcagtcagg aactgaaccg
    57121 cgaagccgct ctggcccggc tggtggcagt gattaaagat ttaacaaaag aacaaaaagc
    57181 ccgacgaccc acgcggccca cccgtgcatc gaaagagcgc aggctggcat cgaaagcaca
    57241 aaaatcaagc gtgaaggcga tgcgcggcaa agtacgcagc ggtcgggaat aaaaagaagg
    57301 aatggatggt gaaaaaagcg atagtgacag cgatggctgt aatcagcctc tttactctga
    57361 tgggatgtaa taatcgggcc gaagtcgata cgctttctcc ggcgcaggct gccgaactga
    57421 aaccgatgcc gcaaagttgg cgcggcgtgc taccgtgtgc cgattgcgaa ggaatcgaaa
    57481 tctctctgtt cctcgaaaaa gacggaacat gggtgatgaa tgagcgttat ctcggtgctc
    57541 gtgaagaacc ttcctccttc gcttcctacg gtacatgggc gcgaaccgct gacaagctgg
    57601 tattaaccga cagcaaaggt gaaaagtcat attatcgggc gaaaggcgat gcgctggaga
    57661 tgctcgatcg tgaaggcaat ccaattgaat cgcagttcaa ctatacgctg gaaccggcac
    57721 aatccagttt acctatgacg ccgatgaccc tgcgaggcat gtatttttat atggctgatg
    57781 cggcgacctt cactgattgc gcgaccggaa aacgtttcat ggtagcgaat aacgcagagc
    57841 tggagcgtag ctacctggct gcgcgcggtc acagtgaaaa accggtgtta ctgtcagtag
    57901 aaggtcactt tacgcttgag gctaatccgg atactggtgc gccaaccaaa gtattagcgc
    57961 ccgatacggc gggtaaattt tacccgaata aggattgcag tagtttgggg cagtaaacct
    58021 gcttaaggca gaaacaaacg caaaattgcc tgatgcgctg cgcttatcag gcctacagca
    58081 tataatgcaa cattttgaat tagcacgatt ttgtaggccg gataaggcgt ttacgccgca
    58141 tccggcattt tgtgcacgag gctggcttta tcactgagtc agcccaacaa agcgacccgc
    58201 cttgatataa atcccaggct tcaggtgccc aacatactgt aactgcgttt ctggcttaaa
    58261 cgcagagaca tcaccaatac gcatatgcat cagatcggca gggctaatcc agcccgattg
    58321 cgccagtgta agtggatgcc cggatttggc aaatgcatca gtgacaaatt ccgaacaaaa
    58381 ccacgacttt ttgtctcctt cgcacacact gcttaactgc gctttcgcca ggtcgctgac
    58441 gcactgttgg cgaaaatcct cggagaacgg attcagtgag cacatctggc gagtcaccat
    58501 aaagggaata aattcgacaa tgccgcgata gttataaccg ctatctttga ttttattggc
    58561 aaacgcggtg atatctgtgg cttgttgcgg ggtaagatcc gggactcgta agacgaaaag
    58621 cttatcacta tgcttcatcg cttttttaag ggaaacaatc tggacgccag cgcctgtcgc
    58681 ttctgcaacg ttattatcac ccagaaagat tgcaacgtga ctcacagagg aagtgctgaa
    58741 gacgcggatt ccaaatgagg ttaccccaag gcttgaggag aacagcaaat cgccgggttt
    58801 gagatcaggt gccgttattt cttttattga ttgttcggtg aaagagcttt gatgctggaa
    58861 tttgacagcc catgtttttg cctcggcatc tactgctgtg gctgatgggt ctggctggct
    58921 gatatcaacg gtacaagccg aaagtaaaag aaagctgggg agaagcaggc ggcagtacgc
    58981 ctttggttta tccattttat acaatccatg taaaaaaggg ccctgaaatt caggaccctt
    59041 tctggcatca gcctttaatc tgtttcacca gatattcgac gatgtcacca gtcttaatta
    59101 actgtttctc gccgttgcga cgatatttat attcgatatc gtcgttgtcg aggttacggt
    59161 cgcccagcac aatagtgtgc ggaataccga tcagttccat atcagcaaac atcacgcccg
    59221 gacgctcttt gcggtcatcc agcagcactt cgataccttg tgcacgcagt tcgctgtaca
    59281 gtttctcagc aagctcttgt acgcggaagg atttgtgcat gttcatcggc agaatcgcca
    59341 cctggaacgg cgcgatagcg tcaggccata cgatgccgcg ttcgtcgtag ttctgctcaa
    59401 tcgccgcagc taccacacgc gttaccccga taccgtagca acccatcgtc aggatttggt
    59461 tacggccatc ttcaccctgt acggaggctt tcagtgcttc ggagtacttg gtacccagct
    59521 ggaagatgtg accaacttcg ataccacgtt tgatcagcag cgtaccctgg ccatccgggc
    59581 ttggatcgcc agccaccacg ttacggatat ctgcaacttc cggggtagcg acatcgcgat
    59641 cccagttgat accgaagtag tgtttaccat cgatgttagc accagcagcg aaatcactca
    59701 tcgccgcaac ggtacggtca atcaccaccg gaatcggcat gtttaccgga cccagtgaac
    59761 ccggaccggc tttaaccaca gcacgaattt cttcttcggt cgcgaaagtc agcgggcttg
    59821 caacctgcgg cagtttttct gctttaactt cgttcagctc gtgatcaccg cgcaccagca
    59881 gcgcaaccag cgggaagcta ctgccttcaa ccgctttaac cagcagagtc ttaaccgttt
    59941 tctcaatcgg cagattgaac tgttcaacca gttccgcgat ggttttcgcg ttcggcgtat
    60001 caaccagcgt catttcctgg gtagcagcag cgcgcggttc tttcggcgcg atagcttctg
    60061 ccagttcaat gttcgctgca tagtcagagg tgtcggagaa gaccacatcg tcttcaccgc
    60121 tctgcgccag cacctggaat tcgtgagagg cgctgccgcc gatagaaccg gtgtcggcct
    60181 gtacggcgcg gaaatccagc cccatgcggc tgaagatttt gctgtaggcc gcatacattg
    60241 catcgtaggt ttcctgcagg gattcctgag aagtatggaa agagtaagca tctttcatca
    60301 ggaattcgcg ggaacgcatg acgccgaaac gcggacgcac ttcgtcgcgg aacttggtct
    60361 ggatctgata gaagttcagc ggcagctgtt tgtaagagct aagctcgtta cgaatcaggt
    60421 cagtgataac ttcttcatga gttgggccga gtacgaacgg acgctcgcca cggtcaacaa
    60481 aacgcagcag ttccggaccg tactgttccc aacgaccact ctcttgccac aaatcggctg
    60541 gctgaaccac cggcatcgac acctcgatcg caccggcgtt gttcatctct tcacgcacga
    60601 tgttttcgac ttttttcaga acgcgcacgc cggtcggcag ccaggtatat aacccggagg
    60661 ccagcttgcg gatcatcccg gcgcgcagca tcagctgatg gctgatcacc tcggcgtcgg
    60721 caggtgtctc cttgagagtg gagagcaggt attggctagt acgcatgttg ttacggttcc
    60781 agttggaagg tagaacaggc tcaaggcgag cctgggacaa aaaaagtgat ttagtttacc
    60841 agtgcaaaag aaatgtcaaa agagaagggc gtgaatttaa cgcggttcca gcgcaaagac
    60901 ttcaaaacct gcgtcggtga cgcgccagcg aacgttaaaa tcatgtagcc agacggcata
    60961 ggttttgccc gtttcctcac ctttacgata ggccgggcgc gggtcctgcg ccagtacttc
    61021 gcggataaac agcgttaact gcggataacg cttctccagc gtcaaaagct gcttttcgac
    61081 ctctgcggta aaactcaccg ccatctctgc agctggcgca ctttgcgcat agctggcact
    61141 ggcatcggga agcgattcgg caaaggggag atacggtttg atatccacta ccggcgtacc
    61201 atcgaccaga tccagactgc cgagcttcag aatcacgctg cctttatggc aaacaacctc
    61261 tttcagctct accagcgaca tgccaattgg gttagggcgg aaagtagagc gtgttgcgaa
    61321 aacccccatt ctggcgttac cgccgaggcg cggtggacgc acagtcggac gccagccgcc
    61381 ttccatcgtt tgatgaaaga cgaaaaggat ccataaatgg ctgaatgctt ccaggccgcg
    61441 aacggcgtcg gcctggttgt aggaagctat gagatgcagt tcgccgttgg cgctttttac
    61501 cagacctggc tggcgcggaa cggcgaattt ttctttatag ggcgagcgaa taacgcctat
    61561 ttgctcgaac tggaaactgc tcatttcgcc gtaatgttaa gcgcagagcc gatacataca
    61621 gcctgacgat agcagcccgg cgtaccgctg gtgacttcgc agctatgcag taatacagca
    61681 ttggctttca ttttagaggc attgatttgc atctgcttac gtgcggttgg aatgctcggc
    61741 ggagagtcct gattagaggc ctggcaagag tcgccactga cttcaccgag atcgcggaac
    61801 ggtttgccga ctaattcttc tgcattggta taaattcgga ccggcgtggc gcgcggcgct
    61861 ttcggttttg caggctccgc tttcggctgg ggtgcagtgc tttgaacggg ttcgacaggg
    61921 gatctgctta acatggaaca gccgcttagc atgagtgcta ctaaacagat cggtaaagca
    61981 cgcatagtat ttcctcaatg tatgatcaaa acgtcaatat tgaatcagga gcttgtaaaa
    62041 atgacaagac gggcaagcgc ccgtcctgaa tgatattaca aattgtggaa acagcctaaa
    62101 aattaccagc ctttaacagc gccgccgtta aacactttgt ttgctgcttc gtaaacttca
    62161 tcagactgat aagcctggac gaatttcttc acgttctcgg cgtctttgtt atcttcacgc
    62221 gttacgatca ggtttacgta tggggagtct ttatcttcga caaagatacc gtctttcgcc
    62281 ggagtcaggc caatctggct ggcataggtg gtattgataa ctgccagagc gatttgcgcg
    62341 tcgtccagag aacgcggcag ttgcggtgct tccagttcaa caattttcag attttttggg
    62401 ttctcaacaa catcaagaac ggtcggcagc aggccaacgc catctttcag tttgatcaag
    62461 cccacttttt gcagcagcag cagtgaacga ccaagattag ttggatcgtt tggcacggca
    62521 acctgcgaac catcctgcag ttcatccagt gatttgattt tcttggagta accagcaatc
    62581 ggataaacaa aagtgttgcc tactgcgacc agtttgtagc cacgatcttt cagttgctga
    62641 tcaaggtacg gtttatgctg gaaggcgttg gcgtcgatat cgcctttgct caatgcttcg
    62701 tttggcagaa catagtcgtt gaaggttacc agctcaacgt ccaggccata tttgtctttc
    62761 gcaactttct gcgcgacttc tgctacctgc tgttcggcac caacaatcac gccgacttta
    62821 atgtggtttg gatctttttc atcctgaccg cagcctacca gtgccagtga tccaatcagg
    62881 gctcccactg ccgcaaaggt tttgaatttg aacgccatac cttattcctt cttcaattat
    62941 ttatgttgtg ttgaacgtta cttgcgagtg acagcccgga cgatgcggtc gcctgcaaac
    63001 tgaattaaat aaaccagaat gaccagcaat accagtaccg tattcatcac cgtcgcgttg
    63061 tagccgatgt agccatactg atagccaatc tgacctaaac caccggcacc gactgcacca
    63121 cccatcgcgg aataaccgac cagggtaatc agggtgatag ttgccgcatt caccagaccc
    63181 ggcaacgctt ccggtaacag cactttacag acgatctgca ttggcgtggc acccattgcg
    63241 cgggaagctt caattaaccc ggttgggatc tccagcagag cgttctcgac catacgggca
    63301 ataaacggtg ctgcaccaac ggttaacgga acaattgctg cctgcaaacc aatcgatgta
    63361 ccgacaataa cgcgggtaaa cggaatcatc cataccagca agataatgaa cgggatggaa
    63421 cggaaaatgt tcacaatcgc agaaacggta cgatacagct tcgcgttagc aataatttgc
    63481 cccggacgcg tgacataaag cagaacgcca accggcagac caatcacaaa cccaaaaaat
    63541 ccggatacga aggtcattgc cagcgtttcc catacgccac gaaccagcag ccacatcatc
    63601 ggctcagaca taacccagta cctctacttt tacatggtgt tcctgcagcc aggcaatggc
    63661 ggcttgcgta tcttgttgtg tgccgtgcat ttcagtcagc atgatgccga atttaacgcc
    63721 accggcgtag tccatctgcg cgctaataat gttgttgttg acgttgaaac gacgcgcggt
    63781 ttcagaaagc agtggggcat cgaccgattg accggtaaac tccagacgca gcatcggcac
    63841 gcagtcagta aatggctccg tttgcagacg ttcctggtaa tcttccggga tatccagatg
    63901 cagggtcgac tgaataaact tctgcgccag cggcgttttc ggatgcgaga acacttcact
    63961 taccgtgtcc tgctcgatca gttcgccatt gctgatgacc gccacgcaat cacaaatacg
    64021 cttcacaacg tccatttcgt gggtgatcaa cagaatcgtc aaacccagac ggcggttaat
    64081 gtctttcagc agttcgagaa tagaacgtgt cgttgccggg tccagcgcgc tggtggcttc
    64141 atcacacagc aatactttgg gattgctggc taatgcacgg gcaatcgcca cacgttgttt
    64201 ctgcccaccg gaaagattcg acgggtagct atcatgctta tcgccaagac caaccaatga
    64261 cagcaattcc gtcacgcgac gtttgatctc gtctttcggt gtgttgtcca gctccagcgg
    64321 cagagccacg ttgccaaaaa cagtacgcga agagagcagg ttaaaatgct ggaaaatcat
    64381 accaatctgg cggcgagctt tggtcaactc ggattctgac agcgtggtca attcctggcc
    64441 atcgaccagc acgctaccct cggttgggcg ctccagcaag tttacacaac gtataagcgt
    64501 actcttaccc gcgcctgagg caccgataac gccataaatt tgtccagctg gcacatgcag
    64561 gctgacgttg ttcaacgcct ggatggtgcg ggtgccctgg tggaacactt tggtgatatt
    64621 cgaaagtttt atcattgatt atttattatc gtcattaagt tagtcgtagc atctcgaatg
    64681 cctgaaacgg gcaacgccgt caatgaaatg gatgttaagg catccagacg tctaaatcaa
    64741 tcaggtttat gcgaagagca ctttcttgca ggtcgaaaca tgcgatacta gcgtcccatg
    64801 ccttattaag gagctataaa aggtggcgaa gagcgtaccc gcaatttttc ttgaccgtga
    64861 tggcaccatt aatgtcgatc acggctatgt ccatgagatc gacaactttg aatttatcga
    64921 cggtgttatt gacgccatgc gcgagctaaa aaaaatgggc tttgcgctgg tggtagtaac
    64981 caaccagtct ggcattgctc gcggtaaatt taccgaagca cagtttgaaa cgctgaccga
    65041 gtggatggac tggtcgctgg cggaccgaga tgtcgatctg gatggtatct attattgccc
    65101 gcatcatccg caggggagtg ttgaagagtt tcgccaggtc tgcgattgcc gcaaaccaca
    65161 tccggggatg cttttgtcag cacgcgatta tttgcatatt gatatggccg cttcttatat
    65221 ggtgggcgat aaattagaag atatgcaggc agcggttgcg gcgaacgtgg gaacaaaagt
    65281 gctggtgcgt acgggtaaac ctattacacc tgaagcagaa aacgcggcag attgggtgtt
    65341 aaatagcctg gcagacctgc cgcaagcgat aaaaaagcag caaaaaccgg cgcaatgatt
    65401 aaaagatgaa cggttgaaat aaaaatgcat ttttccgctt gtcttcctga gccgactccc
    65461 tataatgcgc ctccatcgac acggcggatg tgaatcactt cacacaaaca gccggttcgg
    65521 ttgaagagaa aaaatcctga aattcagggt tgactctgaa ataggaaagt gtgatatacg
    65581 ccacctcgcg acagtgagct gaaagctgcg tcgcaactgc tctttaacaa tttatcagac
    65641 aatctgtgtg ggcactcgaa gatacggatt cttaacgtcg caagacgata aatgaatacc
    65701 aagtctcaag agtgaa
//
LOCUS       DAZPCS010000005        60419 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZPCS010000005 DAZPCS010000000
VERSION     DAZPCS010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60419
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.005"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..322
                     /locus_tag="ACHUQ7_000272"
     CDS             <1..322
                     /locus_tag="ACHUQ7_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1133857.1"
                     /translation="KVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPND
                     SNSVYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQ
                     VTYP"
     gene            435..1229
                     /gene="panB"
                     /locus_tag="ACHUQ7_000273"
     CDS             435..1229
                     /gene="panB"
                     /locus_tag="ACHUQ7_000273"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFR1133858.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1241..2092
                     /gene="panC"
                     /locus_tag="ACHUQ7_000274"
     CDS             1241..2092
                     /gene="panC"
                     /locus_tag="ACHUQ7_000274"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFR1133859.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHNGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(2166..3068)
                     /gene="rpnC"
                     /locus_tag="ACHUQ7_000275"
     CDS             complement(2166..3068)
                     /gene="rpnC"
                     /locus_tag="ACHUQ7_000275"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HFR1133860.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEECLKGHSTDVLYSMQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            3342..3722
                     /gene="panD"
                     /locus_tag="ACHUQ7_000276"
     CDS             3342..3722
                     /gene="panD"
                     /locus_tag="ACHUQ7_000276"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFR1133861.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3726..4955)
                     /locus_tag="ACHUQ7_000277"
     CDS             complement(3726..4955)
                     /locus_tag="ACHUQ7_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFR1133862.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQISDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNTINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATVFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLEKMSRLVSNQPQG"
     gene            complement(5019..5459)
                     /locus_tag="ACHUQ7_000278"
     CDS             complement(5019..5459)
                     /locus_tag="ACHUQ7_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFR1133863.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRECIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5564..6334)
                     /locus_tag="ACHUQ7_000279"
     CDS             complement(5564..6334)
                     /locus_tag="ACHUQ7_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1133864.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6331..7257)
                     /locus_tag="ACHUQ7_000280"
     CDS             complement(6331..7257)
                     /locus_tag="ACHUQ7_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR1133865.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7366..8028
                     /gene="can"
                     /locus_tag="ACHUQ7_000281"
     CDS             7366..8028
                     /gene="can"
                     /locus_tag="ACHUQ7_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFR1133866.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8069..8605)
                     /gene="hpt"
                     /locus_tag="ACHUQ7_000282"
     CDS             complement(8069..8605)
                     /gene="hpt"
                     /locus_tag="ACHUQ7_000282"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFR1133867.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8811..11201
                     /gene="gcd"
                     /locus_tag="ACHUQ7_000283"
     CDS             8811..11201
                     /gene="gcd"
                     /locus_tag="ACHUQ7_000283"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFR1133868.1"
                     /translation="MAINNTASRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11248..12798)
                     /gene="cueO"
                     /locus_tag="ACHUQ7_000284"
     CDS             complement(11248..12798)
                     /gene="cueO"
                     /locus_tag="ACHUQ7_000284"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFR1133869.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMVIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            12964..13311
                     /locus_tag="ACHUQ7_000285"
     CDS             12964..13311
                     /locus_tag="ACHUQ7_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFR1133870.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLSNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13417..14283
                     /gene="speE"
                     /locus_tag="ACHUQ7_000286"
     CDS             13417..14283
                     /gene="speE"
                     /locus_tag="ACHUQ7_000286"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFR1133871.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14299..15093
                     /gene="speD"
                     /locus_tag="ACHUQ7_000287"
     CDS             14299..15093
                     /gene="speD"
                     /locus_tag="ACHUQ7_000287"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFR1133872.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15131..15493)
                     /gene="yacL"
                     /locus_tag="ACHUQ7_000288"
     CDS             complement(15131..15493)
                     /gene="yacL"
                     /locus_tag="ACHUQ7_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFR1133873.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15668..18265)
                     /gene="acnB"
                     /locus_tag="ACHUQ7_000289"
     CDS             complement(15668..18265)
                     /gene="acnB"
                     /locus_tag="ACHUQ7_000289"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFR1133874.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVNVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLSSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLAEIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18620..20473
                     /locus_tag="ACHUQ7_000290"
                     /pseudo
     CDS             18620..20473
                     /locus_tag="ACHUQ7_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
     gene            complement(20544..21968)
                     /gene="lpdA"
                     /locus_tag="ACHUQ7_000291"
     CDS             complement(20544..21968)
                     /gene="lpdA"
                     /locus_tag="ACHUQ7_000291"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFR1133875.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATLPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22293..24173)
                     /gene="aceF"
                     /locus_tag="ACHUQ7_000292"
     CDS             complement(22293..24173)
                     /gene="aceF"
                     /locus_tag="ACHUQ7_000292"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFR1133876.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAKAEGKSEFAENDAYVHATPLIRRLAR
                     EFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKVDFSK
                     FGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAKRKLD
                     VKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVVPVFK
                     DVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVNAPEV
                     AILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSDIRRL
                     VM"
     gene            complement(24188..26851)
                     /gene="aceE"
                     /locus_tag="ACHUQ7_000293"
     CDS             complement(24188..26851)
                     /gene="aceE"
                     /locus_tag="ACHUQ7_000293"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFR1133877.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKELSTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27012..27776)
                     /gene="pdhR"
                     /locus_tag="ACHUQ7_000294"
     CDS             complement(27012..27776)
                     /gene="pdhR"
                     /locus_tag="ACHUQ7_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFR1133878.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMVGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28317..29690
                     /gene="aroP"
                     /locus_tag="ACHUQ7_000295"
     CDS             28317..29690
                     /gene="aroP"
                     /locus_tag="ACHUQ7_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFR1133879.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29733..30587)
                     /gene="ampE"
                     /locus_tag="ACHUQ7_000296"
     CDS             complement(29733..30587)
                     /gene="ampE"
                     /locus_tag="ACHUQ7_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFR1133880.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30584..31135)
                     /gene="ampD"
                     /locus_tag="ACHUQ7_000297"
     CDS             complement(30584..31135)
                     /gene="ampD"
                     /locus_tag="ACHUQ7_000297"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFR1133881.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31223..32116
                     /gene="nadC"
                     /locus_tag="ACHUQ7_000298"
     CDS             31223..32116
                     /gene="nadC"
                     /locus_tag="ACHUQ7_000298"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFR1133882.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIVSGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32319..32759
                     /gene="ppdD"
                     /locus_tag="ACHUQ7_000299"
     CDS             32319..32759
                     /gene="ppdD"
                     /locus_tag="ACHUQ7_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFR1133883.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSSIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            32769..33916
                     /locus_tag="ACHUQ7_000300"
                     /pseudo
     CDS             32769..33916
                     /locus_tag="ACHUQ7_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
     gene            33906..35108
                     /gene="hofC"
                     /locus_tag="ACHUQ7_000301"
     CDS             33906..35108
                     /gene="hofC"
                     /locus_tag="ACHUQ7_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFR1133884.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(35143..36186)
                     /gene="guaC"
                     /locus_tag="ACHUQ7_000302"
     CDS             complement(35143..36186)
                     /gene="guaC"
                     /locus_tag="ACHUQ7_000302"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFR1133885.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGQVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(36348..36386)
                     /locus_tag="ACHUQ7_000303"
                     /pseudo
     CDS             complement(36348..36386)
                     /locus_tag="ACHUQ7_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="too short; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            36411..37031
                     /gene="coaE"
                     /locus_tag="ACHUQ7_000304"
     CDS             36411..37031
                     /gene="coaE"
                     /locus_tag="ACHUQ7_000304"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFR1133886.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37031..37774
                     /gene="zapD"
                     /locus_tag="ACHUQ7_000305"
     CDS             37031..37774
                     /gene="zapD"
                     /locus_tag="ACHUQ7_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFR1133887.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAVSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            37784..37981
                     /gene="yacG"
                     /locus_tag="ACHUQ7_000306"
     CDS             37784..37981
                     /gene="yacG"
                     /locus_tag="ACHUQ7_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFR1133888.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38081..38470)
                     /gene="mutT"
                     /locus_tag="ACHUQ7_000307"
     CDS             complement(38081..38470)
                     /gene="mutT"
                     /locus_tag="ACHUQ7_000307"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFR1133889.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGGPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(38530..41235)
                     /gene="secA"
                     /locus_tag="ACHUQ7_000308"
     CDS             complement(38530..41235)
                     /gene="secA"
                     /locus_tag="ACHUQ7_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFR1133890.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPITEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41297..41809)
                     /gene="secM"
                     /locus_tag="ACHUQ7_000309"
     CDS             complement(41297..41809)
                     /gene="secM"
                     /locus_tag="ACHUQ7_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFR1133891.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNPNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42040..42957)
                     /gene="lpxC"
                     /locus_tag="ACHUQ7_000310"
     CDS             complement(42040..42957)
                     /gene="lpxC"
                     /locus_tag="ACHUQ7_000310"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFR1133892.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43058..44209)
                     /gene="ftsZ"
                     /locus_tag="ACHUQ7_000311"
     CDS             complement(43058..44209)
                     /gene="ftsZ"
                     /locus_tag="ACHUQ7_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFR1133893.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44270..45532)
                     /gene="ftsA"
                     /locus_tag="ACHUQ7_000312"
     CDS             complement(44270..45532)
                     /gene="ftsA"
                     /locus_tag="ACHUQ7_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFR1133894.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(45529..46359)
                     /gene="ftsQ"
                     /locus_tag="ACHUQ7_000313"
     CDS             complement(45529..46359)
                     /gene="ftsQ"
                     /locus_tag="ACHUQ7_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFR1133895.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLVRFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46361..47281)
                     /gene="ddlB"
                     /locus_tag="ACHUQ7_000314"
     CDS             complement(46361..47281)
                     /gene="ddlB"
                     /locus_tag="ACHUQ7_000314"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFR1133896.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47274..48749)
                     /gene="murC"
                     /locus_tag="ACHUQ7_000315"
     CDS             complement(47274..48749)
                     /gene="murC"
                     /locus_tag="ACHUQ7_000315"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFR1133897.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMCVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(48803..49870)
                     /gene="murG"
                     /locus_tag="ACHUQ7_000316"
     CDS             complement(48803..49870)
                     /gene="murG"
                     /locus_tag="ACHUQ7_000316"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFR1133898.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(49867..51111)
                     /gene="ftsW"
                     /locus_tag="ACHUQ7_000317"
     CDS             complement(49867..51111)
                     /gene="ftsW"
                     /locus_tag="ACHUQ7_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFR1133899.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51111..52427)
                     /gene="murD"
                     /locus_tag="ACHUQ7_000318"
     CDS             complement(51111..52427)
                     /gene="murD"
                     /locus_tag="ACHUQ7_000318"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFR1133900.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALVA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLVRYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52430..53512)
                     /gene="mraY"
                     /locus_tag="ACHUQ7_000319"
     CDS             complement(52430..53512)
                     /gene="mraY"
                     /locus_tag="ACHUQ7_000319"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFR1133901.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(53506..54864)
                     /gene="murF"
                     /locus_tag="ACHUQ7_000320"
     CDS             complement(53506..54864)
                     /gene="murF"
                     /locus_tag="ACHUQ7_000320"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFR1133902.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPALV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(54861..56348)
                     /gene="murE"
                     /locus_tag="ACHUQ7_000321"
     CDS             complement(54861..56348)
                     /gene="murE"
                     /locus_tag="ACHUQ7_000321"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFR1133903.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFPASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(56335..58101)
                     /gene="ftsI"
                     /locus_tag="ACHUQ7_000322"
     CDS             complement(56335..58101)
                     /gene="ftsI"
                     /locus_tag="ACHUQ7_000322"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFR1133904.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNSDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALTMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58117..58482)
                     /gene="ftsL"
                     /locus_tag="ACHUQ7_000323"
     CDS             complement(58117..58482)
                     /gene="ftsL"
                     /locus_tag="ACHUQ7_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFR1133905.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58479..59420)
                     /gene="rsmH"
                     /locus_tag="ACHUQ7_000324"
     CDS             complement(58479..59420)
                     /gene="rsmH"
                     /locus_tag="ACHUQ7_000324"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFR1133906.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEEHF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59422..59880)
                     /gene="mraZ"
                     /locus_tag="ACHUQ7_000325"
     CDS             complement(59422..59880)
                     /gene="mraZ"
                     /locus_tag="ACHUQ7_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFR1133907.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLLSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14472 a        16732 c        14849 g        14366 t
ORIGIN      
        1 aaaggttggt actgaaaacg ggcaactcct tggtaatact ctcacaggta atgatgcagc
       61 aaaaggagtc ggtgtactca tagaaggttt agcaactagt aaaaatcctc taatgacatt
      121 gaaacctaat gattcaaatt ctgtttataa agattacgac ccgagaggca aagacgatac
      181 aacaggagga gtttacccgg atcaagatac aggtataaca taccctctcc atttccaggc
      241 cacgctacaa caggatggaa ctataccaat agaagctggt gagtttaaag ccaccagtac
      301 tttccaggta acctaccctt aataagtcca ccgcaccgcc atcatctggc ggtgcggtaa
      361 ttgataaatc attgccccca ccaatgtgac tgataaaatg ccaggcaaca cgacatcatt
      421 tatcaggata cgttatgaaa ccgaccacca ttgcttcact tcagaaatat aagcaggata
      481 aaaagcgttt cgcgaccatc accgcttacg actacagctt cgcgaaacta tttgctgatg
      541 aagggcttaa cgtcatgctg gtaggcgact cgctgggcat gaccgttcag ggccacgatt
      601 ccactctgcc agtaaccgtt gctgatatcg cctaccacac tgccgccgta cgtcgcggcg
      661 caccaaactg cctgctattg gctgacctgc cgtttatggc gtatgccacg ccggaacaag
      721 cctttgaaaa cgccgcaacg gttatgcgtg ccggtgccaa tatggtcaaa attgagggcg
      781 gtgagtggct ggtcgaaacc gtaaaaatgc tgaccgaacg tgccgttcct gtatgtggtc
      841 acttaggttt aaccccacag tcagtgaata ttttcggtgg ctacaaagta caggggcgcg
      901 gcaatgaagc gagcgatcga ctactcagcg atgcattagc cttagaagcc gcgggggcac
      961 agctgctggt gctggaatgc gtgccggttg agctggcaaa acgtattacc gaagcactgg
     1021 cgatcccggt tattggtatt ggtgcaggca acgtcactga cgggcaaatc ctcgtgatgc
     1081 acgacgcctt tggtattacc ggcggtcaca ttcctaaatt cgctaaaaat ttcctcgccg
     1141 aaacgggcga catccgcgcg gctgtgcggc agtatatggc tgaagtggag tccggcgttt
     1201 atccgggcga agaacacagt ttccattaag gagtcacgtt gtgttaatta tcgaaaccct
     1261 gccgctgctg cgtcagcaaa ttcgccgcct gcgtatggaa ggcaagcgcg tggcgctggt
     1321 gcctactatg ggtaatctgc acaatggcca tatgaagctg gtcgacgaag ccaaagcccg
     1381 cgccgatgtg gtcgtcgtca gtattttcgt taacccgatg cagttcgacc gcccggaaga
     1441 tctggcacgt tatccacgga ccttgcagga ggactgcgag aagctaaaca aacgtaaagt
     1501 ggatttagtt ttcgcccctt cggtaaaaga gatctaccca aacggtactg aaacccacac
     1561 ttacgttgac gttcctggcc tttcgaccat gctggaaggt gccagccgtc cgggacattt
     1621 tcgcggcgtt tcgactattg tcagcaagct gttcaacctg gtccagccgg acatcgcctg
     1681 cttcggtgaa aaagattttc agcaactggc gttgatccgc aaaatggttg ccgatatggg
     1741 cttcgatatt gagattgtcg gtgtgccaat tatgcgcgcc aaagacggtc tggcgctaag
     1801 ttcccgtaac ggttatctga cggcggaaca acgcaaaatt gcgcctggtc tgtacaaagt
     1861 tttaagttcg attgctgaca aattgcaggc tggggaacgg gatctcgatg aaattattac
     1921 cattgcgggg caagaactga atgaaaaagg cttccgcgcc gatgatattc agattcgcga
     1981 tgccgacaca ttgctggaag tttctgaaac cagcaaacgg gcagtaattc tggtagccgc
     2041 ctggcttggc gatgctcgcc tgatcgacaa caaaatggtc gagctggcgt aatacttaac
     2101 tggcgctacg gctgatggcg ccagttatta atttacccca cgtcatctgt cataacacag
     2161 gtaacttaaa taaggttaat taccttatca atctcagcaa gaggtaaatt tgccatctct
     2221 gcaacgtctt cccgagacat tcctttactt agaagacgca gggcgaattc ttgcctttcc
     2281 tcctgtcttc cctgctgaat ccccttctca atccctttct cttcaaacca ctgcgccagc
     2341 gtcatcatag acttccctcc cgtttccctg tctctcagca caccataaaa caaatccgct
     2401 tgttcagtat gaccgcgttg cagcatatag ttttgcatgg caactaactg acttccgcta
     2461 gtgtaccctt cgtcaatcag cgtgaccagt tgctccagca ataacattaa gtcgcgctgg
     2521 cgaatatgtt tttgcagtag ttcgagaatc gcaatccgcc gatgttgcat gatttcgtca
     2581 tccggtgtga tggtgatatc caccagcggg aaaggactgt tatagacgcg tcgcgccagc
     2641 tccggcgagt aaaacatatc aaaccagcac attgagagcg gataaggtgt ggcctcgccc
     2701 tgataaaaca gtatcggcac caccagcggc agcttatcgt ggccagcctc cagatgccgg
     2761 tgcatggcag caatagaata acgcatcatg cgaaaggcca ttttcttatc cggcttgctt
     2821 tggtgttcaa tcacaacatg cagataaccg ggattgccct gcatttgcat ggaatagagc
     2881 acgtccgtgc tgtgaccttt caggcactct tcaatgaaac tccccgactc taaatgaagc
     2941 gtgttgagat cacaaagttc gcgtaattcc actggcaaat gtatctccag aaagtcgcga
     3001 gccgtctccg catgcattaa aaattgttta aataccgcgt catgcggtgt ggtactcggt
     3061 gcatccatct tccctgtctt ccgtgtggcc ttgcgtgaag gcagtatcca gtcaaggcgt
     3121 gctatcgtca gccacctctt tattgtttac cgagcagcgt tcataaaaat atcaattcgt
     3181 tacaggcgtt acatggcacg cttcggcgcg gtacgcaaac tgctgacgca aatgccatca
     3241 gatgagtaga cactcatcaa aaatcgggca atactgcgtg agaatttcct caaagcaggc
     3301 accgcctgct tcgttaacga cagggtagaa aggtagaagt tatgattcgc acgatgctgc
     3361 agggcaaact ccaccgcgtg aaagtgactc atgcggacct gcactatgaa ggttcttgcg
     3421 ccattgacca ggattttctt gacgcagccg gtattctcga aaacgaagcc atcgatatct
     3481 ggaatgtcac caacggcaag cgtttctcca cttatgccat cgcggcagaa cgcggttcga
     3541 gaattatttc tgttaacggt gcggcggccc actgcgccag cgtcggcgat attgtcatca
     3601 tcgccagctt cgttaccatg ccagatgaag aagctcgcac ctggcgaccc aatgtcgcct
     3661 attttgaagg cgacaatgaa atgaaacgta ccgcgaaagc gattccggta caggttgctt
     3721 gattgttatc cctgcggctg gttactcacc agccgcgaca tcttctccag cgaatccgtt
     3781 cttaagatat aaagtcgttt tagtaacaac ggattatccc ccggttttac tttgcctttc
     3841 atggttgtca ccgccaggtg aaatccggca tcgtttgctg ccttcacggc tttgtcatta
     3901 aagccgccaa acggatacga aagataccag acatgtggat taaattgcgc cagagcgcgg
     3961 cgtgaacgtg caaaatcaaa cagaatattg tgctcactac ggctcagtaa tatgggtcgg
     4021 cgataaccat ctacccgatg caaaaaatgg gtatgtgact ggaaatcaaa aacatcgcga
     4081 atttcgttaa gctcagaaac gctcataaat tgcagcgatt ttgggttcca cttctgcggg
     4141 tgacgtttga tgcgtgaggt aacaataaac accgtcgcct tcatgccata ttgtttcaac
     4201 acaggatacg catagcggct caccgacttg aggccatcat caaaggtaat caccaccgct
     4261 cgcgcaggga gattgatcgt attcttcacg taaccttcca gctgagccat gctcagtgtc
     4321 gcgtatcccc tgtcgcgcag ccaggccatt tggttattga aagcgcgtac cgaggtggtc
     4381 gtcgaagtat ggcgaaaacg ggtgttttct tcgtcgcgca gaatatggtg ataagttagc
     4441 accggcaggc cattatcggg ttgggcatcc agcgcgctga tataggccag tcgatcgcca
     4501 atacggatct gataccaggt ttgatttaac ctgtctttca gtttatgcaa aatcgggtag
     4561 cgcaaattgt ccgccagtac cccaaatggc gcactgccca cactcggcgc gttgtagacc
     4621 ggcgtatctt tccaggtaat taagttctga ttactcagcg gcttgttgag gtcacccaaa
     4681 ccgtcttcaa ctttttgtcg tccctgaact ggctcgagat gccctttatc gataaaccct
     4741 ttaccaaagc caaaattaaa tgcgtaataa cttgcggcag tgggctccac ggcaatgatt
     4801 tgtccggccc gaatattccc cacggtcacc atcttgtcac taatttgcgc ccagaccgca
     4861 gcattttcga tggtttgcat ataacgggca ggtaacgcgg cactgacact tgcggtaaac
     4921 agcatcagca ggagaataac agcttgttta tacatgaaaa aataaccgag ggtgaaacaa
     4981 cgcttttatt gtagcaaaag cgccatcaga atcgcgggtt aatactcata caaattatgt
     5041 ttgaggacga aaggcggatt tttttgttgt tggtgccaaa gactactcac ctccggccca
     5101 cccagttcga caatacactc acgaaactct gaactggtca tggtttcacg gcaagccatc
     5161 atctgttcaa ttaacggtaa cgtgacacca gaaatcactt cgcaacggga gtgtttgtgg
     5221 cttaataatg aagccacgcg atacggtggc gctccggcta tatcggttaa gaagatgaca
     5281 ccctcaccag agtccgcttc atgcagagca tcgcacatca tgcggctgag catattagag
     5341 cttaatccgc gccagaaatt cacggcccgg cactgaagaa gtgccccatg ttttttctcc
     5401 agcgcatcca gtatctcttg cgcccggtca tcgtgacagg taattaccca acctaacatc
     5461 gccttcctcc ttagccgaaa atcagcctac cctgaccatt gttgataaag ggtgatagcc
     5521 gtcaaaactc ctctcccccg aatccagagg agaggaaata gccttagcta cgcaaaccac
     5581 gtccacgttg gatcagcgac caacagatca aataaaacgc cacaataaag accaccagta
     5641 cgccaaaggt agtgaccagc ggcacatcat ttataccgag gaagccgtag cggaaaccac
     5701 tgatcatata aacgattggg ttcaggtgcg acagcccttg ccagaacggc ggcaacaaag
     5761 tcagtgagta aaagaccccg cccaaatacg tgagtggcgt taacacaaag gttggcacca
     5821 ggctgatgtc atcgaacgtt ttggcaaaca cgccgttcag caaacccgca agggagaata
     5881 gcaccgccgt gagcaccagg gttaaggcaa cgaataccca cgaatgcacc tgaaacggca
     5941 caaaaaacag tgaaattgcc gtcacaagaa tgccaacaaa cagaccacgc gccacgccac
     6001 cgccgacata tccggcaata atgacgtgag tcggaaccgg cgctaccagc agctcttcaa
     6061 tattacgctg gaacttggca ccaaaaaatg atgacgcgac gttagcgtag gcgttggtga
     6121 tcaccgacat catgatcagc cccggtacga tgaactgcat atagctgaag ccatgcatat
     6181 cgccaatacg cgaacctatc aggttaccga agataataaa gtaaagggtc atggtgatga
     6241 ctggcggcac cagcgtctgc acccagatac gcataaagcg atggatctct ttcgcccaga
     6301 tgctttttag cgccacccag taaagatgca tcatgcgcga tctccttgtt tttcattaac
     6361 cagtgaaaca aacagctctt ccagacggtt agctttgtta cgcatactta atacctgaat
     6421 gccctgctca cttaactgcg taaatacgct gttgatcccc tgctcacgta gcacttcaac
     6481 ttccagcgtc gcggtatcga ccagtcgata ctgatagcca tcgagcttcg gtaacggact
     6541 tttcggtgcg agatcgagaa taaaggtttc cgatttcagc ttcgccagca gcgccttcat
     6601 cgaggtattt tccaccagct caccatgttg aataatgccg atattgcggc acagcatttc
     6661 tgcttcttcc aggtagtgtg tggtaagaat gatggtggtg cctttgtcgt ttaaatcctt
     6721 caaaaagccc cacattgagc ggcgaagttc aatatccacg cctgcggtcg gttcgtcgag
     6781 aatcagtagt ttaggttcat gcattaacgc acgggcaatc attaaacggc gcttcatccc
     6841 gccagataac atacgcgcac gttcgttgcg ttttccccat aaatcgagct gcttaagata
     6901 tttttcgctg cggatgtacg cctctttgcg ctccacaccg tagtaccctg cctgattcac
     6961 cacaatttgc tgcacggttt caaacgggtt gaagttaaat tcctgcggca ccagtcccaa
     7021 ctgacgttta gcgttcacga catctttctc gagatcgtaa ccaaatacgc tgacccgccc
     7081 ggaggtttta tttaccagag agctgataat accgatagtg gtcgatttcc cggccccgtt
     7141 cggcccgaga agcgcataaa aatcacccgc ttcgacctgc aaatctattc cacgaagcgc
     7201 ctgaacgccg cctggatagt tttttttaag ctgttgaagt tccagtgcaa tggtcataaa
     7261 tttttactta ccttacgttc ttacacttta tctgtggttt aaatcgtccc ggagttgccc
     7321 tatattagcc aaacgtaatt atttggttac aggtcgttaa cctccatgaa agacatagat
     7381 acactcatca gcaacaatgc actatggtca aaaatgctgg tggaagagga tcccgggttt
     7441 tttgagaaac tggcacaagc gcaaaaaccg cgctttctat ggattggatg ttccgacagt
     7501 cgcgttcctg cagaacgttt aaccggtctt gagccgggcg aactctttgt tcaccgtaat
     7561 gttgctaacc tagtcattca caccgacctg aactgccttt ccgtggttca gtatgcagtg
     7621 gatgtactcg aagttgaaca cattattatc tgtggccact acggttgcgg cggcgtacaa
     7681 gccgccgttg aaaacccgga actggggctt atcaacaact ggctgctgca tatccgcgat
     7741 atctggttca aacatagctc attgctcggc gaaatgccgc aagagcgccg tctggatacc
     7801 ttgtgtgaac tgaacgtcat ggaacaggtg tataacctgg gccactccac cattatgcaa
     7861 tcagcgtgga aacgcgggca gaaagttacc attcacggct gggcctacgg cattcacgac
     7921 ggcttgctgc gtgatctgga tgttaccgcc accaaccgcg aaacccttga gcaacgttac
     7981 cgtcacggga tttccaacct caagctgaaa cacgccaacc acaaataaaa atgccatgcc
     8041 ggatgcaaca catccggcaa cttcacactt actcgtccag cagaatcact ttgccaatat
     8101 acggcagatg acggtaacgc tgtgcgtaat caatgccgta acccaccaca aactcatccg
     8161 ggatcgagaa accgataaat tcaaccggga cgttcacttc acgacgggac ggtttatcca
     8221 gcagcgtaca aatcgccaac gacttcggtt cgcgcaggct taagatctca cgcactttcg
     8281 acagtgtatt ccccgagtcg atgatatctt caacaatcag cacgtccttg ccacggatat
     8341 cttcatccag atctttgagg attttcacat cacgggtggt ggacatgccg ctaccgtagc
     8401 tggaggcggt cataaagtcg acttcatgag atacctgaac ttcacggcac aggtccgcca
     8461 taaacataaa tgagccacgc agcagaccca ccagcaccat atcgctgccg ctgtctttgt
     8521 aacgctcagt aatctgacga cccagttcgg cgatacgcgc tttaatctcc gcttcgggga
     8581 tcattacttc tacagtatgt ttcatatctc taaccatatg atttaaaaac aaatcacttc
     8641 gcacgggcag gttttggcac cgcaacaaag cgcaagacgc gcagtatacc agtaaaacta
     8701 ttcattcgcg gaatctgtta ataaagctcg aattgtgatg acggtcacac atgttaaaac
     8761 ctataattaa ttgctactaa aatattaatg aattgaaatg gtgtctcttt atggcaatta
     8821 acaatacagc ctcgcgacga ttactcgtca cgctaacagc cctttttgca gcgctttgcg
     8881 ggctgtatct actcattggc ggaggctggc tggtcgcgat tggcggctcc tggtactacc
     8941 ctatcgctgg ccttgtgatg ctcggcgtcg cctggatgct gtggcgcagt aaacgcgccg
     9001 cgctttggct atacgcagcc ctgctgctcg gcaccatgat ttggggcgtc tgggaagttg
     9061 gtttcgactt ctgggcgctg actccgcgca gcgatattct ggtcttcttc ggcatctggc
     9121 tgatcctgcc gtttgtctgg cgtcgcctgg tcattcctgc cagcggcgca gttgccgcac
     9181 tggtggtcgc actgctgatt agcggtggta tcctgacctg ggccggattt aacgatccgc
     9241 aggagatcaa cggcacctta agcgccgatg ccacacctgc cgaagctatc tcccccgttg
     9301 ccgatcagga ctggcctgcg tatggccgca atcaggaagg tcaacgcttt tcaccactga
     9361 agcaaattca cgccgataac gtccacaagc tgaaagaagc ctgggtgttc cgtactggcg
     9421 atgtgaagca gccgaacgat ccgggtgaaa tcaccaatga agtgacgccg attaaagtgg
     9481 gcgataccct ttacctgtgt accgctcacc agcgcctgtt tgcgcttgat gccgccagcg
     9541 gcaaagagaa atggcattac gatcctgagc tgaaaaccaa cgagtctttc cagcacgtaa
     9601 cctgccgtgg cgtctcttat catgaagcca aagcagaaac ggcttcgccg gaagtgatgg
     9661 cggattgccc gcgtcgtatc attcttccag tgaatgatgg tcgcctgatt gcgattaacg
     9721 ctgaaaacgg caaactgtgc gaaaccttcg ccaataaagg cgtgctcaat ctgcaaagca
     9781 atatgccaga caccaaaccg ggtctgtatg agccgacttc tccgccaatt atcaccgata
     9841 aaaccatcgt gatggccggt tcagtcaccg ataacttctc aacccgcgaa acgtctggcg
     9901 tgatccgtgg ttttgatgtc aacaccgggg agctgctgtg ggcttttgat ccgggcgcga
     9961 aagatccgaa cgcaatcccg tcagacgaac acacctttac ctttaactca ccaaactcgt
    10021 gggcaccagc ggcctatgac gcgaagctgg atctggtcta tctgccgatg ggcgtgacca
    10081 cgccggatat ctggggcggt aaccgcacac cggaacagga acgttatgcc agctcgattc
    10141 tggcgctgaa tgccactacc gggaaactgg cgtggagcta ccagaccgtt caccacgacc
    10201 tgtgggacat ggatcttccg gcacagccga cgctggcgga catcaccgtt aatggtcaga
    10261 aagtgccgat tatttacgct ccggcgaaaa ccggtaacat ttttgtgctc gatcgtcgta
    10321 atggcgaact ggtggttccg gccccggaaa aaccggttcc ccaaggtgct gccaaaggcg
    10381 attacgttac ccctactcaa ccattctcgg aactgagctt ccgtccgacg aaagatttga
    10441 gcggtgcgga tatgtgggga gccaccatgt ttgaccaact ggtgtgccgc gtgatgttcc
    10501 accagatgcg ctatgaaggc attttcacgc cgccatctga acagggtacg ctggtcttcc
    10561 cgggtaacct ggggatgttc gaatggggcg ggatttccgt tgatccaaat cgtgaagtgg
    10621 caattgccaa cccaatggca ctgccgtttg tttcgaaact gctcccgcgt ggtcctggca
    10681 acccgatgga gcagccgaaa gatgccaaag gcacgggtac ggaatccggc attcagccac
    10741 agtacggtgt accgtatggt gtcacgctca acccgttcct ctcaccattt ggtctgccat
    10801 gtaaacagcc agcatggggt tatatctcgg cgctggatct gaaaactaat gaagtggtgt
    10861 ggaagaaacg tattggtacg ccgcaggaca gtatgccgtt cccgatgccg gttccggtgc
    10921 cgttcaatat gggtatgccg atgctgggcg ggccaatctc cacggcgggt aacgtgctgt
    10981 ttatcgccgc tacggcagat aactacctgc gcgcttacaa catgagcaac ggtgaaaaac
    11041 tgtggcaggg tcgtttaccg gcgggtggtc aggcaacacc gatgacctat gaagtgaatg
    11101 gcaagcagta cgtggtgatc tccgcaggcg gtcacggttc atttggtacg aagatgggcg
    11161 actatattgt ggcttatgcc ctgccggatg atgtgaaata accataatgc ccggagagat
    11221 ccgggcatat ttccgaatac ggtcttttta taccgtaaac cctaacatca tccccgtatc
    11281 ttcatgctcc agcagatggc agtgcgccat ataagcatgt tctttcggtg cgttgtgatt
    11341 aaacttcacc agcacttcac tgacattgcc ttctacctta acggtatctt tccagcccgc
    11401 gcgatgagcc gctggcggtt tgccattttc tgacaagata cggaactgcg tgccgtgaat
    11461 atggaacgga tgcagcatca tgtcgcccac gccagagata acccaacgtt cgtactgccc
    11521 tttcgccgcc gcaaacatcg gcttattcat atcaaacgcc tgaccgttga ttttattggc
    11581 atggtggaaa tcgaacttcc cgccgtggtt catatgattc atattgccgt gccccatatg
    11641 gcccatcatc tggctgtgat ccattccggc catcgcctga tcgccatatt tctccattag
    11701 catctgcatc cccatcatat cgagcatcgg gtccatagaa agttgcagct tgcgtaccgt
    11761 cagcccttcc agcgaaggta acgcaggcag gctacttaat gtgtcaggca aagcaccgga
    11821 ggcactaata gcaatcggct gaatccgcat taccggatgc ggcttgtcaa acggtgcaat
    11881 caccatcccc atctggctga ccggcagcgt caccaggtca aagggtttgt tgtcgttaac
    11941 ctccaccagc acttcaaaac gctcgcccat cagcaccggc agctcgctca ccttcaccgg
    12001 ttcaggtagc agaccaccgt cgctggcaat cacatacagc gggcgattgt cgctggtggc
    12061 gaaattgagc gagcgggcat tacagccatt gagcaaacgc aggcgcagcc aaccacgcgg
    12121 ggcagcgtgt tgtgggtaga ttgcaccgtt ggtcagcagc gtatcgccaa accagcccac
    12181 ggcggcggtc atcacatcca gttgataatc aatctgcccg tcggcgctaa atttcttatc
    12241 ctgaacgatc accggaacat catcgatacc ccactgtttt ggcagcatta atttcaggat
    12301 ctcgtcatct tcaatcacca ccagaccagc cagccccatc gccacctgtc gcccggtctt
    12361 gccatgttga tgtggatgga accagcaggt agcggcaggt tgatcaacgt tcaacgtcac
    12421 cgagcgcttg ccacctggcg gaataattcc ctgtgggccg ccgtcgacgt cacccggtac
    12481 ttccagcccg tgccagtgca acgtcgtctc ttccgccagt tggttgtaga tatcaaccgt
    12541 taccgctttg ccgcgctgta atttcaccgc cggccccagc agattgccgt tatagcccca
    12601 ggtagttgca gttttctcgc caaaggtgga ctgacctgcg ccaatagtta actgaatgcg
    12661 attacgggca tcggtcgtga gcaaatcagg gatcggtaac gttgggcgtt ccgccgcaaa
    12721 gactgcacgg ctccacagtg gcaaggctga agccacaccc agtgcgacgg aatatttcaa
    12781 gaaatcacga cgttgcatag ttatttcctt attcttaagc aggcgaaaca aaatcaaacg
    12841 ttgagcatag tccttcccct tacgggaagg tcaagccgca gacacaattt aatcgacaat
    12901 aatggtcgtc gcctcgctcg cagtgtgcta acgtttatct tctttaagcc cctggtagaa
    12961 gcaatgaaga cgtttttcag aacagtgtta ttcggcagcc tgatcgccgt ttgcgcaaac
    13021 agttacgcgc tcagcgagtc tgaagccgaa gatatggccg atttaacggc agtttttgtc
    13081 tttctgaaga acgattgtgg ttaccagaac ttatctaacg ggcaaattcg tcgcgcactg
    13141 gtctttttcg cccagcaaaa ccagtgggat ctcagtaatt acgacacctt cgacatgaaa
    13201 gctctcggtg aagacagcta ccgcgatctc agcggcattg gcattcccgt cgctaaaaaa
    13261 tgcaaagccc tggcccgcga ttccttaagc ctgcttgcct acgtcaaata atccctgata
    13321 cctttttgta gaaatattga ccgtgcatcc gcggtcaatg ttagctatta tgttgcgccc
    13381 tttttttacg ggtgttaaca aaggaggtat caacccatgg ccgaaaaaaa acagtggcat
    13441 gaaacgctac acgaccagtt tgggcagtac tttgcggtag ataacgttct gtatcatgaa
    13501 aagaccgatc accaggatct gatcattttt gagaacgctg catttggtcg cgtaatggcg
    13561 ctggatggcg tagtacaaac caccgagcgc gacgagttta tctatcatga gatgatgacc
    13621 catgttccgc tactggccca cggtcacgcg aaacatgtgc tgattatcgg cggcggcgac
    13681 ggtgccatgc tgcgtgaagt aacccgacat aaaaacgttg agtcaatcac gatggtggaa
    13741 atcgatgcgg gtgtcgtgtc gttctgccgt cagtatctgc ccaaccataa cgccggtagc
    13801 tacgacgatc cgcgctttca actagtgatc gacgatggcg tcaatttcgt taatcaaacc
    13861 agccagacct ttgatgtcat tatctccgac tgcaccgatc ctatcggtcc cggcgaaagc
    13921 ctgttcacct cagcatttta tgaaggctgc aaacgttgcc tgaatcctgg cggtatcttc
    13981 gtcgcacaaa acggcgtctg ctttttacag caggaagaag ccatcgacag ccatcgcaaa
    14041 ctcagccatt acttcagcga cgttggcttt tatcaggcgg cgatcccgac ctattacggc
    14101 ggtatcatga cttttgcatg ggcgacagat aacgacgcct tacgccatct ctcaaccgaa
    14161 attattcagg cgcgttttct cgcttctggc ctgaaatgcc gttattacaa tccggcaatc
    14221 catacggcag cttttgcctt acctcagtat ctgcaagacg cactggcttc acagccgtcc
    14281 taaggagaag ataagaaatt gaaaaaactg aaactgcatg gctttaataa tctgaccaaa
    14341 agtctgagtt tttgtattta cgatatctgc tacgccaaaa ctgccgaaga gcgcgacggt
    14401 tatattgctt atatcgatga actctataat gccaaccgtc tgaccgaaat cctgtcagaa
    14461 acctgttcca ttatcggggc caatattctt aacatcgccc gccaggatta cgaaccacag
    14521 ggtgccagcg tcactattct ggtgagtgaa gaaccggttg acccgaaact catcgacaaa
    14581 acagaacacc ccggcccact gccagaaacg gtggttgctc atctcgataa aagtcatatt
    14641 tgcgtacata cctacccgga aagtcatcct gaaggcggtt tatgtacctt ccgcgccgat
    14701 attgaagtct ctacctgcgg cgtgatttct ccgctgaagg cgctgaatta cctgatccac
    14761 cagcttgagt ccgatatcgt aaccattgat tatcgcgtgc gcggttttac tcgcgacatt
    14821 aacggtatga agcactttat cgaccatgag attaattcga ttcagaactt tatgtctgac
    14881 gatatgaagg cgctgtatga catggtggat gtaaacgtct atcaggaaaa tatcttccat
    14941 accaagatgt tgcttaaaga gttcgacctt aagcactaca tgttccacac caaaccggaa
    15001 gatttaaccg acagcgagcg ccaggaaatt accgctgcgc tgtggaaaga aatgcgcgag
    15061 atttattacg ggcgcaatat gccagctgtt taacggctct ggcggagctc ccaggctccg
    15121 ccagatttat ttacttctgc tgcacgaaat tgcggtaagc cgccacgacc tgcagaaaat
    15181 cctcaacgcc gcatagcgac aggctttctt cgtcgtagta gttcatcccc tcttccattt
    15241 catcgccagc gaattccagt tgattggcgc gaaccatcac ctcttcagcg tccatccaca
    15301 gggtgtattc atgccctgcc cgttgccagg accgttcgct acctttcagt gcgtgcgctg
    15361 cttgttccac ttcatcaagc aaggccaggt tttctttcac ctcttcatta aaccagtgcc
    15421 cgaccacttc atgccccatg gacatacgca cctttaccac tccggtaata tcgcgcagaa
    15481 attcgtaatc catagtgtgt tcctctgctc ccggcaatgc gccgaaatcg catcgcgcca
    15541 ttgatgtaat tatcgcagcc ttaccggaga aaaacagcgg aacggcgata gtttagaaat
    15601 aaaaaatgcc cgggaatctc attcaccggg cattgtgtcg tttatgcgca gcgcgtgcgc
    15661 ttacttatta caccgcagtc tggaaaatca ccccatcggc tttttcggtg tactgagaaa
    15721 gctggttgaa gttcagataa cgataagtat caacggcagt tttatctact tgcgcaacgt
    15781 aggtctggta ctcttccggc gtcggcagtt tgccaatcag cgccgcaaca gccgccagtt
    15841 ctgcagaagc caggaagaca ttcgcgccag tacccaaacg gttcgggaag ttacgggtag
    15901 aggtggaaac caccgtcgca ccgtctgcta cacgcgcctg gttacccata cacagggaac
    15961 agcccgggat ctcgatacga gccccgctct taccgaagac gctgtagtag ccttcttccg
    16021 tcagttgcgc ggcgtccata cgggttggcg gtgccaccca caggcgggtc ggcaactgac
    16081 ctttgtgcgc atccagcagt ttaccagcgg cacggaagtg accgatgtta gtcatgcaag
    16141 aaccgataaa tacttcgtcg atcttctcgc cctgtaccgc agacagcgga cgtgcgtcat
    16201 ccggatcgtt cggagcacac aggattggct ctttaatctc cgccagatcg atgtcgatca
    16261 ctgccgcgta ttccgcatct gcatcggctt ccagcagctc aggattcgcc agccattttt
    16321 ccatgccctg aatacggcgt tccagggtac gacgatcgcc gtaaccttcc gcgatcatcc
    16381 acttcagcag gacgatgttg gagctcaggt attcgatgat cggttctttg ttcagcttga
    16441 tagtacaacc agcggcagaa cgctcggcgg acgcatcggt tagctcaaag gcctgctcaa
    16501 ctttcagatc cggcagacct tcaatttcca gaatgcgacc agagaagatg tttttcttgc
    16561 ctttcttctc aacggtcagc agaccttgtt tgatcgcata cagcggaatg gcgtgtacca
    16621 gatcgcgcag ggtgatgccc ggctgcattt tgcctttgaa gcgcaccaga acggattccg
    16681 gcatatccag cggcattacg ccagttgcgg cagcaaacgc caccagacca gaacccgccg
    16741 ggaaagagat accgatcggg aaacgggtat gggagtcacc accggtaccg acggtatccg
    16801 gcagcagcat acggttcagc caggagtgaa tgacgccgtc acccggacgc agcgacacac
    16861 cgccacggtt cataatgaag tccggcagcg tgtggtgcgt gttcacgtca actggcttcg
    16921 gatacgccgc ggtgtggcag aaagactgca tcaccaggtc agccgagaag cccaggcacg
    16981 ccaggtcttt cagttcatca cgggtcatcg ggccggtggt gtcctgagag cctacagaag
    17041 tcattttcgg ttcgcagtac gcgcccggac gaatgccttt cacgccacag gcacggccta
    17101 ccattttctg cgccagcgag aagccgcgat cgctctcagc gacatctttc gcctgacgga
    17161 acacatcact gtgcggcaga ccaagtgctt cacgcgcttt ggtggtcagg ccacgcccga
    17221 taatcagcgg aatacggccg ccagcacgca cttcatcaat cagcacgtcg gttttcagtt
    17281 cgaaggtcgc cagcagttcg cctgtttcgt ggttacgcac ttcacctttg tacgggtaaa
    17341 cgtcaatcac gtcgcccatg ttcaggttag agacgttgac ttcgattggc agtgcacccg
    17401 cgtcttccat cgtgttaaag aagatgggtg caattttacc gccgaggcac aaaccaccgc
    17461 cgcgtttgtt cggcacatgt ggaatatcat cgcccataaa ccacagaacg gagttcgtgg
    17521 cggatttacg cgatgaaccc gtacccacaa cgtcaccgac gtacgccagc gggaaacctt
    17581 tctgttgcag agcttcaatc tgtttgatcg ggccaacaac acctggctgg tctggctcaa
    17641 taccttcacg ggcgtttttc agcatcgcca gcgcgtgcag tgggatatcc gggcgtgacc
    17701 acgcatccgg tgccggagaa aggtcatcgg tgttggtttc gccagtgact ttaaagacgg
    17761 taacggtcag tttttcagcc agcgccgggc gattcaggaa ccattcggca tccgcccagg
    17821 actgcattac ctgcttcgca tattcgttgc ctgctttcgc tttctcttct acgtcataga
    17881 agttatcgaa catcagcagc gtgtgagaaa gtgctttggc agcaataggt gccagtttgg
    17941 catcatccag cgcgtcgatc agcggatgaa tgttgtaacc accctgcatg gtgcccagca
    18001 gttcgatagc tttttccgga gtcagcagag gggatttagc ttcgccttta gcgatagcag
    18061 ccaggaagcc tgcttttaca taggcggctt catcgacgcc tgggggaaca cggttggtta
    18121 acagatctaa caggaattct tcttcgcccg cgggcgggtt tttcagcagc tctacaagcg
    18181 cggccatttg gtttgcatcc aggggtttgg gcgcaatccc ctcagcggca cgctcagcta
    18241 cgtgcttacg gtattcttct agcacgacgg ttctcctcgc tctcattgtc atagtgcggc
    18301 aggcgattct cttcacgctc ctgtgagaca gcagtttgta gggtaaatgc ccagtaccgc
    18361 aacgggcaga atagcaggat tttgacgacg tgttaatctg tttacaaaaa agcaacataa
    18421 aaaagtggct gaatcgttaa ggatggtcta gagattgttt ccctccgtaa aaacttcgtt
    18481 aattcgcatg gtgataatca cctttctcaa cgaatcccaa gattagtcaa aatttaaact
    18541 accgcctctt tatactcgga ttcacagcac ctgcgggtgg cagttcgccg accattgcga
    18601 tttccttgag atccgaatta tgaaaatgac tttgccgttt aaaccccatg tgctggcgct
    18661 aatttgcagt gccgggcttt gtgctgcctc tgccgggcta tatataaaaa gccgcacagt
    18721 ggaagcgcct gtagaaccgc aatcgacaca actggctgtg tctgacgctg ccgctgttac
    18781 gcttcctgca acggtttccg cacctcccgt aatacccgca gtcgtcaaat ccgcattcag
    18841 cactgcacaa atagatcaat gggtcgcacc cgtcgcgctg tatcccgacg ccctactttc
    18901 gcaggtgctg atggcatcaa cctatccgac aaacgttgct caagcagtgc aatggtcgca
    18961 cgataatcca cttaaacaag gcgatgctgc tattcaggcg gtatctgacc agccgtggga
    19021 cgccagcgtt aaatcactgg tggcctttcc acaattgatg gcattgatgg gcgaaaaccc
    19081 gcaatgggtg caaaacctgg gcgatgcttt tctggcgcag ccgcaggacg tgatggactc
    19141 ggtacagcga ttgcggcaac tggcgcaaca aaccggctcg ctgaagtcat caaccgaaca
    19201 gaaagttatt accacaacga agaaagctgt accggtaaaa cagacagtca cggcacccgt
    19261 cataccatcc aataccgttt taactgccaa ccccgtcatt acagagccgg caacaaccgt
    19321 catttccatt gagcccgcca atcctgatgt ggtctatatt cccaactaca acccaaccgt
    19381 ggtttacggg aactgggcca atactgcgta tccgccggtt tatctgccac caccagccgg
    19441 agaaccgttt gttgacagct ttgtgcgcgg attcggctat agcatgggcg ttgctaccac
    19501 gtacgcacta ttcagcagca tcgactgaga tgacgacgat catgaccatc atcatcatga
    19561 cgatgatgat tatcatcacc acgatggcgg tcatcgtgac agtaatggct ggcaacacaa
    19621 cggcgacaac atcaatatcg acgtcaacaa tttcaaccgt atcaccggtg agcatcttac
    19681 tgataagaat atggcatggc ggcacaatcc aaactaccgt aatggtgtgc cctatcatga
    19741 tcaggatatg gcaaagcggt ttcatcaaac cgatgtcaac ggcggaatga gtgccacgca
    19801 gctacctgct ccaacacgcg acagccagcg tcaggcggca gcaagtcagt ttcagcaacg
    19861 aacacacgcc gcccccgtca ttacacgaga tacccaacgt caggcagcgg cacagcggtt
    19921 taatgaagct gaacactatg ggagctatga cgacttccgc gacttcagcc gtcgccaacc
    19981 actgacccag cagcaaaagg acgccgctcg tcagcgttat cagtcggcct ctcctgagca
    20041 gcgccaggca gtccgcgaga aaatgcagac taacccgcag atccagcagc gaagggaggc
    20101 ggcgcgccag cgtattcagt ccgcctcgcc tgagcagcgc caggcggttc gcgagaaaat
    20161 gcagactaac ccgcagaacc agcagcgaag agaggcagcg cgtgagcgta ttcagtctgc
    20221 aactcccgaa cagcgccagg tgtttaagga aaaagtacag cagcgcccac tgaaccaaca
    20281 gcaacgtgat aacgcccgcc agcgtataca atcggcatca cctgaacaac gtcaggtttt
    20341 tcgggagaaa gttcaggaga gccgcccaca acgtctaaac gacagtaacc atactgtcag
    20401 gctgaataac gagcaacggt cagcagtacg cgaacgtctc tctgaacgtg gagcaagaag
    20461 actggaaagg taaattgcag acgtaaaaaa agcggcgtgg ttagccgctt ttttaattgc
    20521 cggatgttcc ggcaaacgaa aaattacttc ttcttcgctt tcgggttcgg caggtcggta
    20581 atgctacctt cgaacacttc tgccgccagg cccacagact cgtgcagagt cgggtgcgcg
    20641 tggatggtca gtgcgatgtc ttcagcatca caacccattt cgattgccag gccgatttca
    20701 cccagcagct cgccgccgtt ggtaccaaca atcgcaccac cgatcacacg gtgagattct
    20761 ttgtcgaaaa tcagcttggt cataccgtct gcgcagtcag aagcgatagc acgaccagaa
    20821 gcagcccacg ggagggtggc ggtttcatag ctgatgcctt tctctttcgc ttctttctca
    20881 gtcagaccta cccatgcaac ttctggttcg gtataggcga tggacgggat aactttcgga
    20941 tcgaagtagt gtttcttacc ggcgataact tcagcggcaa cgtgaccttc gtgaacacct
    21001 ttgtgtgcca gcatcggctg accgacgata tcgccgatag caaagatgtg cggtacgttg
    21061 gtacgcagct gtttgtcaac gcggatgaaa ccacggtcgt caacttccac gccagctttg
    21121 cctgcgtcga ggtttttacc gttcggcaca cgaccaatcg ctaccagcac ggcgtcgtaa
    21181 cgctgcggtt cagctggtgc ttttttgcct tccatcgtca cataaatacc gtcttctttc
    21241 gcttcaacgg cggtaacttt ggtttccagc atcaggttga atttcttgct gatacgcttg
    21301 gtgaagactt taacgatgtc tttgtcagct gccgggataa cctggtcgaa catttcaacc
    21361 acgtcaatct gtgaacccag cgcgtggtaa acggtgccca tttccagacc gatgataccg
    21421 ccacccatta ccagcaggcg ttctggtact tctttcagtt ccagcgcgtc agtggagtcc
    21481 cagatacgcg gatcttcatg cggaataaac ggcagttgga tcgggcgaga acccgctgca
    21541 atgatcgcat tgtcgaagtt gatcacggtt ttgccgttct caccttcaac ttccagggtg
    21601 ttagccccgg tgaatttacc cagaccgttg accactttga ctttgcggcc tttcgccata
    21661 ccagccagac caccggtcag ctgattgatc actttctctt tccaggtacg aatcttgtca
    21721 atatcggttt tcggttcgcc gaagacgata ccgtgttcag ccagcgcttt ggcttcttcg
    21781 ataacttttg ctacgtgcag cagtgcttta gaagggatac agccgacgtt caggcaaaca
    21841 ccgccaaggg tgttgtaacg ttctacgatt acggtttcca gacctaaatc agcgcaacgg
    21901 aaggcagcgg agtaacctgc ggggcctgcc ccaagtacca cgacctgagt tttgatttca
    21961 gtactcatca tgacctctat atatttatct ccggcggtca tacccgtcgt ctttcaggcc
    22021 gcagcggcgt tagcttcacg ccctcacccc agtcacttac ttatgtaagc tcctggggat
    22081 tctggcgctt gctaccttgc tgcacccaga aatccatagg gtatgtgaag taatacccta
    22141 accaccaccg ggtcgttcta tccgtcggta ttttacaaaa ttgttaacaa tttttaaaca
    22201 acaaacggca accgatttgt ctattcgcta ataacctcaa tacattcatg agattaccag
    22261 aaaaaagccg gccgttgggc cggctctttt acttacatca ccagacggcg aatgtcagac
    22321 agcgtgttgt taatgatggt aatgaaacgg gcaccatcag caccgtcgat cacgcggtgg
    22381 tcgaaggaga gagaaatcgg cagcatcaga cgcggcacga actctttacc attccacacc
    22441 ggctccatcg cggacttgga aacgccgagg atagccactt ccggcgcgtt cacaatcggc
    22501 gcgaagtggg tagtacccag gccgccgatg ctggagatgg tgaagcaacc gccctgcatt
    22561 tcgcccgcag tcagcttacc gtcacgcgct ttcttagaaa tagtcatcag ctcgcgagac
    22621 agctcgatga tgcctttctt gttgacgtct ttgaataccg gaacaaccag accgttcggg
    22681 gtatccaccg ccacaccgat gttgatgtat ttcttcaggg tcagacgctg accgtcttcc
    22741 gacagcgaac tattgaagcg aggcatctgc tcaagagctg cagcaacggc tttcatgatg
    22801 aagacaaccg gggtgatctt cacatccagc ttacgtttcg ccgcttcttc gttctgctgt
    22861 ttacggaacg cttccagctc ggtgatatcg gttttgtcga agtgggtaac atgcgggatc
    22921 atcacccagt tacggctcag gttagcacca gaaattttct ggatacggcc cagttccact
    22981 tcttcgattt cgccaaactt gctgaagtcc accttcggcc acggcagcat gccagggata
    23041 ccaccgccag tcgccgccgg agctgcttct gcacgtttga tagcttcttt cacgtaagcc
    23101 tgaacgtctt cgcgcaggat acgaccttta cggccagtgc ccttcacttt cgccaggtta
    23161 acgccaaact cgcgtgccag acggcggatc agcggggtcg cgtgaacgta agcgtcgttt
    23221 tcagcaaatt cagatttgcc ttccgctttc gcagccggag cttcagcttt tgctgccgga
    23281 gccggcgctg ccgcttcctg tttcgcagga gctgccgcag gcgctgcgcc ttcaacttcg
    23341 aagatcataa tcagcgaacc agttttcact ttatcgccaa cgttgacttt cagttccttc
    23401 acgacgcctg caaacggagc cggaacttcc atagaagctt tatcgccttc tacggtaatc
    23461 agtgactgtt cagcggcaac tttgtcgccc actttcacca tcacttcggt cacttcaact
    23521 tcgtcaccgc cgatatccgg aacgttaact tctttcacgc cagctgctgg tgcaggggtc
    23581 gctgccggag tcgcttcctg tttagccgcc ggagctgccg cgcctgcttc acccgcgact
    23641 tcgaagacca taatcagcga gccggtagac actttgtcac ccacgttcac tttgatctct
    23701 ttcacggtac ctgcaaacgg agccggaact tccatagaag ccttgtcgcc ttctacggtg
    23761 atcagcgact gttcagcttc aactttatcg ccaactttca ccaggatttc ggtcacttca
    23821 acttcgtcgc tgccgatatc cggaacgtta acgtcttttg ccgccgcagc cgcgggtgct
    23881 gctgccggag ctgcttcttt cttctcttct gcctgagcag gtgcagcgtc tgctgcaccg
    23941 tcggcggaat cgaaaatcat aatcagtgcg ccggtctggg ttttatcgcc aacagagact
    24001 ttgatctctt taacgatacc cgcctgcgga gatggaactt ccatagaggc tttgtcgcct
    24061 tctacggtga tcagcgactg ttcggcttca actttgtcgc ccactttgac caggatctcg
    24121 gtgatttcaa cttcatcagc cccgatgtcc ggtactttga tttcgatagc cattattctt
    24181 ttacctctta cgccagacgc gggttaactt tatctgcatc gatgttgaat ttggcgattg
    24241 cgtcagcaac cactttctta tcgatttcgc cacgtttagc cagttcgccc agcgccgcaa
    24301 ccacgacata agaagcatca acttcgaagt ggtgacgcag gttctcacgg ctgtcggaac
    24361 gaccgaagcc atcagtaccc agtacgcgat agtcgtcagc cggtacgtaa gtacggacct
    24421 gctcagcgaa cagtttcata tagtcggtag atgccactgc cggcgcgtcg ttcatcacct
    24481 gagcgatata cggaacgcgc ggagtttcca gcggatgcag catgttccag cgttcacaat
    24541 cctgaccatc acgcgccagc tcggtgaagg aggtcacgct ataaacgtca gaacctacgc
    24601 cgtaatcttt cgccaggatc tctgccgctt cacggacgtg acgcaggatg gaaccggagc
    24661 ccagcagctg aactttacct ttgctacctt caatggtttc gagtttgtag atacctttac
    24721 ggataccttc ctcagcacct tccggcattg ccggcatgtg gtagttttcg ttcagcgtag
    24781 tgatgtagta gtaaacgttc tcttgttttt caccgtacat acgctccaga ccgtcatgca
    24841 taatgacagc aacttcgtaa gcgtaagccg ggtcgtaaga gatacagttc gggatagtca
    24901 gcgactgaat gtggctgtga ccatcttcgt gctgcagacc ttcaccgttc agggtggtac
    24961 gaccggaagt accgccgatc aggaagccac gcgcttgctg gtctccagcc gcccagcaca
    25021 gatcgccgat acgctggaag ccgaacatcg agtaatagat gtagaatggg atcatcggca
    25081 gattgttcgt gctgtaagag gtcgccgctg ccagccagga acaccctgcg cccagctcgt
    25141 tgatcccttc ctgcagaatc tgacctttct cgtcttcttt atagtaagca acctgctcgc
    25201 ggtcctgcgg ggtgtactgc tgaccgttcg ggctgtaaat accaatctga cggaacagac
    25261 cttccatacc gaaagtacgc gcttcgtcgg cgatgatcgg taccagacga tctttgatcg
    25321 acttgttctt cagcatcacg ttcagagcac gaacgaaagc gatagtggta gagagctctt
    25381 tgctctgctc ttccagcagc gcgccgaagt cttgcaggct cggcagctca agcttctcgg
    25441 tgaagttcgg ctgacggctt ggcagataac cgtgcagttt ctgacgctga gcgtgcagat
    25501 aggtatgctc ttcagaacct tccgggaagg tgatgtacgg cagtttttcg atatctgcat
    25561 cagacaccgg cacattgaaa cggtcgcgga tgtgacgcac gccgtccatg ttcattttct
    25621 taacctggtg cgcgatgttt ttaccttcag ccgcgtcgcc catgccgtaa cctttaatgg
    25681 tatgagcaag gattactgtc gctttgcctt tggtttcctg cgctttcttg aatgcagcgt
    25741 agattttctt cggatcgtga ccaccacggt tcagtgccca gatctgctcg tcagtccagt
    25801 ctgcaaccag tgctgcggtt tcaggatatt taccgaagaa gtgttcacga acgtacgcac
    25861 catctttcga tttgaaggtc tggtagtcgc cgtcaacggt ttcgttcatc agctggatca
    25921 gtttaccgct ggtatcttta cgcagcagtt catcccaacg gctaccccac atcactttga
    25981 tcacgttcca gccagcacct tcgaagatgc cttccagttc gttgatgatc ttgccgttac
    26041 cggtgaccgg gccgtcaaga cgctgcaggt tacagttgat aacgaaaacc aggttatcca
    26101 gtttttcacg ggtagcgatg gtgatcgcac ctttggattc cggttcgtcc atttcaccgt
    26161 cgccgaggaa cgcgtaaacg gtctgtttag aggtatcttt caggccacgg tgttccagat
    26221 atttcaggaa tttagcctgg taaatagcac caatcggacc cagacccata gatacggtcg
    26281 ggaactgcca gaattccggc atcagtttcg ggtgcggata ggaagagagg ccattgccgt
    26341 gaacttcctg acggaagtta tccagctgct cctgagtcag acgaccttcc aggaaagcac
    26401 gggcgtaaac gcccggggag atgtggccct ggaagtaaac caggtcgccg ccatcctgct
    26461 cgttgcgtgc acggaagaag tggttaaagc acacatcata aatggttgcg gaagactgga
    26521 aggacgccat gtggccgccc agttcgaggt cttttttcga cgcacgcagc accgtcatga
    26581 tggcgttcca gcggatagct gaacgaatac ggcgttccag ttccagatta cccggatact
    26641 ccggttgttc ttcaacgggg atggtgttga tgtagttgct gatacctgtg cctgcggcta
    26701 cgtttacacc gcctttgcgg gcttcagcaa gcagttggtc gatcagatac tgagcacgct
    26761 caacaccttc ttcacggatg accgattcga tcgcctggag ccagtcgcga gtttcgatcg
    26821 gatccacgtc atttgggaaa cgttctgaca tgggttattc cttatctatc taataacgtt
    26881 gagttttctg gaacctgtcc cattgaactc tcgccagaaa gctcaataag acaggttcta
    26941 cgtttagtta ccgcgctttt atatgcgctt gatttacaac atcttctgga taatttttac
    27001 cagaaaaatc actaattctt tcgttgctcc agacgacgca gagaacgctc acggcggctc
    27061 tcttcacggc ttctgtcgag caaaatttct tcgataaagg ccagatggcg atgcgatgct
    27121 tcgcgcgctt cttccggctt accgaccata atcgcttcaa atatgcgggt gcggtgacta
    27181 ctcaccagcg gcagcatctc gcgacgcgaa tagagcaatt cgaagttctg gcggacattc
    27241 tgggccaaca tcggctccat acaccttagc agatgaagca gaaccacatt gtgggccgct
    27301 tcggtgacgg caatctgata ctggagtacg gcgtttgatt ccgcgtccag atcgccagac
    27361 tgctgcgcca gctctatggc gtggtggagt tcacggatgc gttccttgtc ttcatcggta
    27421 ctacgcagcg cggcgtaata agcggcgata ccttccaggg cgtgtcgtgt ttcgagcaag
    27481 tcatactgtg attcaggatg gtcggagagc agctccacca gcggatcgct gaagctttgc
    27541 cataggctgc tctggacaaa agtgccgcca ccctggcgac gaagcaacaa gcccttcgct
    27601 tcgagacgtt gaatcgcctc acgcaaggag ggacgggaga cgtcgaactg ttttgccagt
    27661 tcgcgttccg gtgggagttt ttcgcccggg cggagagtgc cttcgaggat caaaaactcc
    27721 agttgctgct caatcacatc ggagagtttt ggttggcgga ttttgctgta ggccatgagt
    27781 tcctgtctta agccacttgc cgaagtcaat tggtcttacc aatttcatgt ctgtgacgct
    27841 aaagtaacaa agtattcacc ttatgtccat acaggttttg attgaaatca tgaaactgtg
    27901 cacattttaa caacttgaca tatataacgt ttcaaagttg taactatgca caaatgtaga
    27961 ctttacgtag gaaagggatt ttttaacgat tattaaacta taaaaatccg aattgaaccg
    28021 attcacttac caattttttg atttttaagt caataaaaac gaatttaaat tcactctgca
    28081 tattgagagg ggttgaggct gagctttaca aacggtttct ttttaagcaa ctcatcttca
    28141 accatgcata aagcgggtgc attcgctgcc gcataccatt attcttgatc tgacggaagt
    28201 ctttttgtaa caattcaaac ctccttgatg taaacaaatc aatacaacaa acggaattgc
    28261 aaacttacac acgcatcact gcgtagatca aaaaaacaat caccgcacga ggtttcatga
    28321 tggaaggtca acagcacggc gagcagctaa agcgcggcct taaaaaccgc catattcagc
    28381 ttatcgcgct gggtggcgcg atagggacag ggctattcct aggtagcgcc tccgtaatac
    28441 agtccgcagg gccagggatt atcctgggtt acgccattgc tggttttatc gcctttctga
    28501 tcatgcgtca gctgggtgaa atggtggtcg aagaacctgt cgcaggctcc tttagccact
    28561 ttgcttataa atactggggc agttttgccg gtttcgcctc tggctggaac tactgggtac
    28621 tgtacgtttt agttgccatg gcagagctga ctgccgtggg taaatacatt cagttctggt
    28681 atccggaaat cccaacctgg gtttctgccg ccgtattctt tgtggtgatt aacgccatca
    28741 acctgaccaa cgttaaagtg tttggcgaga tggagttctg gtttgccatt atcaaagtta
    28801 ttgcggtagt agcgatgatc atcttcggcg gctggctgct gttcagtggc aacggcggcc
    28861 cgcaggcgag cgttagcaac ctgtgggatc aaggtggttt cctgccgcac ggcttcaccg
    28921 gactggtgat gatgatggcg attatcatgt tctcgttcgg tggtctggaa ctggtgggga
    28981 tcaccgcagc agaagctgat aacccggagc aaagtatccc gaaagcaact aaccaggtta
    29041 tctaccgcat cctgattttc tatattggtt cgttagccgt tctgctctca ctgatgccgt
    29101 ggacccgcgt taccgccgat accagtccgt ttgtgctgat cttccacgag ttaggcgata
    29161 cctttgtggc gaatgcgctg aacatcgtgg tactgactgc ggcgctctcc gtgtacaaca
    29221 gctgcgtata ttgcaacagc cgtatgctgt ttggtctggc acaacagggg aacgcgccaa
    29281 aagcgctggc gtctgtcgat aaacgcggcg taccggttaa caccattctg gtgtctgcgc
    29341 tggttacagc attgtgcgta ttgattaact atcttgctcc ggaatccgca tttggcctgt
    29401 taatggcact ggtggtatcc gcactggtga tcaactgggc gatgatcagt ctggcgcata
    29461 tgaagttccg tcgcgccaag caggaacaag gcgtggtaac tcgcttccct gctctgcttt
    29521 atccgctggg taactggatc tgcctgctgt ttatggcggc ggtactggtg attatgctga
    29581 tgaccccagg aatggcgatt tcggtatacc tgatcccggt atggctgatc gtgttaggta
    29641 tcggctatct gtttaaagag aaaaccgcca aagccgtaaa agcgcattaa tctctctacg
    29701 ccctcacccg tacagggtga gggcaataat ctctacacca acgccccgta aatcgtcagt
    29761 agcgcaatca ccaccacgac cacgaacgag gttttcttcg ccattgaaac cgctgccttc
    29821 ggcgtctcca ccttatcgac atgcggttca cgcgccagag agaactgcgc cagacgcgtt
    29881 aatacctgat attgcgaagt atggaaatca cccagcgaag caaaccaggc cggtaacgct
    29941 ttctcaccat gaccgatcaa ggcatatacc acacccgcaa gacgaaccgg cacccaatcc
    30001 agtacatgaa gcactgcatc aatgccggac tgtaaacgat gatgcggcgt ctggtatcgc
    30061 gccagccagt attgccatgc acgcaagaac gcatacccca tcagcgtaac gggtccccag
    30121 gttcccccca caatcagcca gaacagcggt gcaagataaa aacgaaagtt aatccacagc
    30181 aatgcatttt gcagctcacg caaatactca cgttcgtcgc agcctgccgg gacgccgtga
    30241 atcatggtga gttcgccagc catcgtggca cgggcatggc tatcattacg tgaagcagct
    30301 gtcagataag catgataatg aagacgaact ttacctgcgc caatacacag caaaccaatc
    30361 agcagccaca ccagcagcgt gggaacgttg aacaatactc cctgcaatgc gcgtaacagt
    30421 aaaaaagtca cacccatcgc aataatggtc atgcctaacg tgcgcccgag agaaaaatgt
    30481 ttcacccgcc gaaagaacgc ttcaagacga tgatcaagct gccagtgctc gcccaactta
    30541 aacaggcgct cgaaaattaa caccagtaag gttgtaaata gcgtcatgtt gtctccttgc
    30601 tgaccagcgc acgaaaccgt gcccaatcaa atgcaggacc gggatcggtt ttccgatccg
    30661 gcgcaatatc acaatggccc gtcatgttat tagcgatatc gggatagcga tctatcagtg
    30721 cccgcgtaac cgccgcaagc tgttgatact gcgcatcggt atacgccaga gtatcggtgc
    30781 cttcaagctc aatcccaata gaaaaatcat tgcagcgttc acgcccctga tactgagaga
    30841 ctcccgcatg ccaggcacgt ttatcgaaag gaacatactg gactatttca ccatcacggc
    30901 gaatcaaaca gtgagcggag acgcgcaaat gggcgatctc agcaaagaaa ggatgtgcct
    30961 gcggatcaat agttccagtg aataatgcgt cgatccacgg accgccaaac tcgcctggcg
    31021 gcaggctaat attgtgcacc accagcaggg tgggtgtttc gtcatccagg cggcaatcgt
    31081 aatgtggtga gggaacgcgg cgcgcgccaa ccagccaccc ctgttctaac aacatgcagg
    31141 atctccttat atgtggtgct aatacccggt tcagagtagc atgtttctac cttatgattc
    31201 gttagctatc tggagtttta acatgccgcc tcgccgctac aaccctgaca cccgacgtga
    31261 cgagctgctg gaacgcatta atctcgatat ccccggcgcg gtggcccagg cgctgcggga
    31321 agatttaggc ggaacagtcg atgccaacaa tgacattacg gcaaaacttt taccggaaaa
    31381 ttctcgctct catgccacgg tgatcacccg cgagaatggc gtattttgtg gcaaacgctg
    31441 ggttgaagag gtgtttattc aactggcagg cgacgatgtc accataatct ggcatgtgga
    31501 tgacggcgat gtcatcaacg ccaatcaacc cttgttcgaa cttgaaggcc catcccgcgt
    31561 gctgttaacg ggcgaacgca ctgcgcttaa ttttgtgcaa accctttcag gagttgccag
    31621 taaggtacgc cactatgtcg aattactgga aggcaccaac acgcagttgt tggatacgcg
    31681 caaaacctta cctggcctgc gttcagctct gaaatacgcg gtactttgcg gcggcggagc
    31741 gaatcaccgt ctggggcttt ctgatgcctt cctgatcaaa gaaaaccata ttattgtctc
    31801 cggctcagtg cgccaggcgg tcgaaaaagc gtcctggctg catccggatg cgccagtaga
    31861 agtcgaagta gagaatctgg aagaacttga tgaggccctg aaagccggag ccgatatcat
    31921 catgctggat aacttcgaaa cagaacagat gcgcgaagcc gtcaaacgca ccaacggcaa
    31981 ggcgctactg gaagtgtctg gcaacgtcac tgacaaaaca ctgcgcgaat ttgccgaaac
    32041 gggcgtggac tttatctccg tcggtgcgct aactaaacac gtacaagcac tcgacctttc
    32101 aatgcgtttt cgctaattgc tgacagattt atccgctcca tcgcgggcgg ataatctccc
    32161 cttccccaac tttcttcgta acgcctcgca aatttcttgt ctttcgctgc aacgaaataa
    32221 aaaacctcgg aatctgcttt cccgtttcgt tttttgcccc tcgcctgtgc tgatggcgtt
    32281 tgccaaagta gcaccaacca aatcaaggag cgaaacagat ggacaagcaa cgcggtttta
    32341 cacttatcga actgatggtg gttattggca tcattgccat tttaagctcc attggtattc
    32401 ccgcttatca aaactacctg cgcaaagccg cactcaccga catgctacaa acctttgtgc
    32461 cttaccgtac cgccgtagag ttgtgcgcgc tggaacatgg tggattagat acctgcgacg
    32521 gtggcagcaa tggcattccc tcgcctacca ccacccgcta tgtttcagcc atgagtgtgg
    32581 caaagggcgt ggtgtcgctg accgggcaag aaagtctcaa tgggctaagc gtcgtcatga
    32641 cgccaggttg ggataacgca aacggcgtca ccggctgggc gcgcaactgc aatattcaaa
    32701 gtgacagcgc attgcagcaa gcctgcgaag atgtcttccg ctttgatgac gccaactaag
    32761 gagcggcaat gaatattcca cagctcacgg ccctgtgcct gcgttatcag ggagtcttgc
    32821 tggatgccag cgaagaagtg gttcatgttg cggtggtcga tgccccctca catgagttgc
    32881 tggacgcatt gcatttcgct accaccaaac gtattgagat cacctgctgg acgcgccaac
    32941 aaatggaagg tcacgccagt cgcacacaac agacattacc cgtagctgtt caggagaagc
    33001 atcagcccaa agcagagttg ctgactcgaa cgttacaatc tgcgctggaa caacgcgcgt
    33061 ctgatattca tatcgaacca gcggacaatg cctaccgcat ccgcttgcgt atcgacggcg
    33121 tattgcatcc tttaccggat gtttcaccgg atgccggagt cgcattaaca gccagattaa
    33181 aagtgctggg aaacctggat attgcggaac atcgcctgcc gcaggacggg caattcactg
    33241 tcgaactggc aggaaacacc gtctcatttc gtattgcgac cttaccatgt cggggtggtg
    33301 aaaaggtggt attaaggttg ttacagcagg tgagccaggc actggatgtt aacacgctgg
    33361 gaatgcagcc gttacaactg gcggactttg ctcatgcctt gcaacaacca cagggactgg
    33421 tgctggtaac tggccctaca ggcagtggca aaacggtcac ctggtgttgt ccacgctaca
    33481 caccaattcc accactgaaa cgctggtacg tttacagcaa atgggggtcg cccgctggat
    33541 gctatcatcg gcgcttacgc tggtaatagc ccagcgtctg gtacgtaaac tttgcccaca
    33601 ttgtcgccga cagcaagggg agcccatcca tattccagac actgtatggc cgtcgccgct
    33661 gccccactgg caggcacccg gttgtgtaca ttgctaccac ggtttttatg gtcgtaccgc
    33721 cttgtttgaa gtcctgccca ttacaccagc catacgccag cttatttccg ctaatactga
    33781 agttgaatcg ctggaaacgc acgcccgaca ggcgggtatg cgtacgcttt ttgaaaacgg
    33841 ctgcctggcc gtggagcaag gcttaaccac ctttgaagag ttaatccgcg tactggggat
    33901 gccgcatggc aagtaagcaa ctctggcgct ggcatggcat aaccggcgac ggcaatgcgc
    33961 aagatgggat gctatgggca gagagccgta ctttactgct tatggcacta cagcaacaga
    34021 tggttacccc actaagcctg aagcgaatcg ccatcaattc tgcgcagtgg cgaggagata
    34081 aaagcgcgga agtcattcat caactggcga cgctacttaa agccgggtta acgctttctg
    34141 aagggctggc tctgctggcg gaacagcatc ccagtaagca atggcaagcg ttgctgcaat
    34201 cgctggcgca cgatctcgaa cagggcattg ctttttccaa tgccttatta ccctggtcag
    34261 aggcatttcc gccgctctat caggcgatga tccgcacggg tgaactgacc ggtaagctgg
    34321 atgaatgctg ctttgaactg gcgcgtcagc aaaaatccca gcgtcagttg accgacaaag
    34381 tgaaatcagc gttgcgttat cccatcatca ttttagcgat ggcaatcatg gtggttgtgg
    34441 caatgctgca ttttgttctg ccggagtttg ccgctatcta taagaccttc aacaccccac
    34501 taccggcact aacgcagggg atcatgacgc tggcagactt tagtggcgaa tggggctggc
    34561 tgctggtgtt gttcggcttt ctgctggcga tagccaataa gttgctgatg cgccgcccga
    34621 cctggcttat cgcgcggcag aaattgctgt tacgcatccc gattatgggt tcactgatga
    34681 ggggacaaaa actcacgcag atctttacga ttctggcgct gacacaaagt gcaggcatta
    34741 cttttttaca gggcgtagag agcgtcagag aaacaatgcg ctgcccgtac tgggtgcaac
    34801 ttctgacaca aatccagcac gatatcagta acggtcatcc catctggctg gcgctaaaaa
    34861 atgccggtga gtttagcccg ctctgtttgc aattagtgag aacaggagag gcatccggct
    34921 cgctggacct catgttagac aacctcgccc atcatcatcg ggataacaca atggcgctgg
    34981 cggataacct cgcagcctta ctggaaccgg cgttgctgat cataacggga ggaattatcg
    35041 gtacgctggt ggtggcaatg tatctgccaa ttttccattt aggcgatgcg atgagtggga
    35101 tgggataacg ctggcgtgtt gctccacgcc agcgttggga gattacaggt tgttgaagat
    35161 gcggttttct tgttccagca cacgaataaa cgtggtgcgc ttggtcagct ctttcaggcg
    35221 tgaagcccca acgtatgtac aagctgaacg caggccgccc aaaatatcac gcgcggtatt
    35281 ttcaacctgg cctcgcagcg gcagcttaac ggttttacct tctgctgcgc ggtattccgc
    35341 aacgccgcca acgtgacgtt tcatcgcaga ctcggagctc atgccgtaga acagcataaa
    35401 tttctcgccg ttctcctcaa cgatgcgacc gccgctctct tcgtggcccg ccagcatgcc
    35461 gccaagcatg acgaaatcgg caccgccgcc aaaggctttc gccacatcgc ccggcgtggt
    35521 acagccaccg tcgctgacga tcattccgcc cagaccgtgc gccgcatcgg cacattcgat
    35581 taccgcagaa agctgcggat aaccgacgcc tgttttgacg cgagttgtac aaacagaacc
    35641 tgggccaatg ccaactttaa cgatatcagc accggagagg ataagctcct cacacatttc
    35701 accagtcact acgttgccag cacaaatggt tttggtcggc cacgcttcac gcgctttcgc
    35761 aacgaactgc acgaagtgtt cggaataacc attcgccaca tcaatacaaa cgaagtttaa
    35821 tgccgggttc aggtcgagaa tctgtttagt tttttcgaaa tccgcatcag acgtgccggt
    35881 agaaaccatc acatgtttca gcacatcagc ggaagaattg ttgataaacg cttgccactc
    35941 ttcgacagaa aagtgtttat gcacagcagt cagaatatca aaagaagcca gcgcagaggc
    36001 catagaaaat gtgcctacgg tgtccatatt tgcggcgata atcggcacgc cggaccagct
    36061 ctgacctgaa tgtttgaagg tgaattgacg ttccagttca acatcggaac ggcttttaag
    36121 agtggagcgt ttagggcgga tgagaacgtc tttaaaacct aacttcagat cttcttcaat
    36181 acgcatgtgc ggattcctgg ggttaatggc tataagggta agagcgcaac tccagtgacg
    36241 ctatcatacg cactaatcaa tgcggcgcaa gactgcgaaa ttgtcttttt ttacgctaca
    36301 atcccataaa tttacctggt aaatagtgag cgaacgtttc atcaatttca ctttcgcgcg
    36361 ctcattttta acgttttgat aatcatgatg aattcccggg aatataaatt atggggtata
    36421 tagttgcctt aacgggaggc attggcagtg gtaagagtac cgttgccaat gcgtttgctg
    36481 atctcggaat taacgtcatt gatgccgata ttattgcgcg tcaggtggtt gaaccaggtg
    36541 cacctgcgct acatgccatt gctgatcact ttggcgaaaa catgattgct gctgatggaa
    36601 cattgcagcg ccgggccttg cgcgagcgga tcttcgccaa cccggaagag aaaaactggc
    36661 ttaacgacct gctgcatccg ctgattcaac aagagacgca acaccagatc cagcaagcaa
    36721 cttcccccta tgtactgtgg gttgtgccat tgctggtaga aaactcactg tataaaaaag
    36781 cgaatcgagt gctggtggtg gatgtcagcc cagaaacgca acttaagcgc accatgcagc
    36841 gcgatgatgt aactcgcgaa catgtcgaac aaatccttgc tgctcaggca acgcgcgaag
    36901 cccgccttgc cgtggcagat gacgtcattg ataataacgg cgcaccggat gctatcgcat
    36961 cggatgttgc ccgcctgcac gcacactatt tgcagcttgc gtcgcagttt gtctcacagg
    37021 aaaaaccgta atgcagaccc aggtcctttt tgaacatcca ctaaatgaaa aaatgcgtac
    37081 atggctgcgc attgagtttt tgattcagca actcaccgtt aatttaccca tcgttgacca
    37141 cgctggcgcg ctgcatttct tccgtaatgt cagtgaatta ctggatgttt tcgagcgcgg
    37201 cgaagtccgc actgagctgt tgaaagaact taaccggcag caacgtaaac tccagacctg
    37261 gattggcgtg cctggcgtgg accagagccg tattgaagca ttaattcagc agttaaaagc
    37321 ggcggtgagc gtattaattt ccgcgccgcg tatcgggcaa tttctgcgtg aagatcgttt
    37381 gattgctctg gtgcgtcagc gactgagcat cccaggcggc tgttgcagct ttgatttacc
    37441 tacattgcac atttggctgc atctacccca ggcgcagcgc gacagccagg tagaaacctg
    37501 gattgccagc ctgaacccgc tcacccaggc acttaccatg gtgctggatt taattcgcca
    37561 gtcggccccc ttccgtaaac aaaccagcct gaatggtttt tatcaggata acggtggcga
    37621 tgccgacttg ctgcgcctga atctgtcgct cgattcacag ctttatccgc aaatttccgg
    37681 acataagagc cgttttgcca ttcgttttat gccgctggac actgaaaacg gacaggtacc
    37741 ggaacgtctg gatttcgaac tggcctgttg ctaaggagta aaaatgtcag aaactattac
    37801 ggtgaattgc ccaacctgcg ggaaaacggt ggtgtggggt gaaatcagcc cgtttcggcc
    37861 attttgctcc aaacgttgtc agctgatcga cctcggagaa tgggctgctg aagaaaaacg
    37921 aatccccagc agtggcgatc tttccgaaag cgatgactgg agcgaagagc caaagcagtg
    37981 acatttgtct gatgcctcac gtaggcctga taagacgcgg acagcgtcgc atcaggcatc
    38041 ttgtgcaaat gtcggatgcg gcgtgaacgc cttatccgac ctacagacgc ttaagcttcg
    38101 caattaccgg ttcattggct ggcggaaaat catcggcatt aagaccgacc agcgacatcc
    38161 actcaccggg ttgcccttct ttaccccacg gccccccttc ccaactttcg accagccaga
    38221 accacagagt tatatgcctg tccgggaatt catattccag tttttcaaat agcgaaaaat
    38281 gttgtggggt aatcccgacc tcttcctgaa gttcacgcac caccgcctgt tccggcgttt
    38341 cgcccatttc aattttaccg ccgggaaact ccagtttatt cgccatgtgc gcatctgctg
    38401 cgcgacgtgt tataaagatt tcattgttct cgttgcgaat aatacctacc gcaatttgca
    38461 gctttttcat tgtcttaaac ctataaaaaa ggcgcagaat cctgcgcctt ttacttcaac
    38521 tgttagcttt tattgcaggc gaccatggca ctgcttgtat tttttaccag aaccgcacgg
    38581 gcaaggatcg ttacgtccta ctttgcgttc accggtttgc gccgccagtg cagctgcggc
    38641 tgcagagtcg tcatcctgat ggctaagctg ctgcatttgt gctaaacgct cggcttccat
    38701 acgacgctgt tgttccagct cctcaacctc ttcaggcata cgtacctgaa ctttgctcag
    38761 cgtactgata acttcatatt tcaacgactc cagcatcgct gcaaacatgg agaacgattc
    38821 acgtttgtat tcctgcttcg gatctttctg tgcatagcca cgcaggtgga taccctgacg
    38881 cagatagtcc atcgctgcca ggtgctcttt ccacagggag tcaagcgttt gcagcatgac
    38941 gcctttctcg aagtgacgca tcatctcagc accaaccact tcttctttac gctgatacac
    39001 ttcgatggac tgcgccagaa tgcgctcacg cagcgtctct tcatgcagtt ctggttcttt
    39061 atccagccac tcggtaattg gtaaatcgag gtcgaaatcg ttcttcagac gttcctgcag
    39121 ccccggaata tcccacattt cttccagcga ctgtggcgga atgtaggcat caatggtggc
    39181 tttgaacaca tcttcacgaa tgctgttgat ggtttcgctc acatcgctga catccagcag
    39241 ttcgttacgc tgggagtaaa tggcgcgacg ctgatcgtta gccacgtcat catattccag
    39301 cagttgctta cgaatgtcga agttacgact ttcaacttta cgctgggcgt tggcaatcgc
    39361 tttagtcacc cacgggtgtt caatggcttc gcctggcttc atacccagtt tacgcatcat
    39421 gccggatact cggtcggaag caaaaatacg catcagcgca tcttccatcg acaggtagaa
    39481 acgggaagaa ccagcatccc cctgacgacc agaacgaccg cgcaactggt tatcgatacg
    39541 acgggattcg tgacgctcgg taccgatgat atgcaggcca cctgcttcca gtaccgcatc
    39601 gtgacgtacc tgccagtcgg ctttaatttt ttcaatttgc tctgcggtcg gattttccag
    39661 cgcggcaact tctgcctgcc agctacctcc gagcacaata tctgtaccac gacccgccat
    39721 gttggtcgcg atagtcaccg cagccggata acctgcctga gcaacaatcg ccgcttcgtt
    39781 ggcgtggaat ttggcgttca ggacattgtg cttaataccg gctttggtca gttcgtttga
    39841 caccagctcc gatttttcga tggagatagt acccaccagc accggctggc ctttcgcagt
    39901 acgttctttg atatcttcaa tgatcgcctg aattttttcc gcttcagtca tgtagaccag
    39961 gtccggcaga tctttacgaa tcattggacg gttggtcgga acaacgacgg tatccagttt
    40021 gtagatggag ctaaattcga aagcttcggt atcagcagta ccggtcatcc ccgccagttt
    40081 ttcatacaga cggaagtagt tctggaaggt gatcgaagcc agcgtttggt tttcgttctg
    40141 gatctgcaca ccttctttcg cttccacagc ctggtgcaga ccatcggacc agcgtcggcc
    40201 ctgcatggta cgaccggtgt gttcgtcaac gatgataact tcaccatctt taacgatgta
    40261 gtcgacgtca cgggtaaaca gcgcatgagc gcgcagcgcc gccgttacgt ggtgcatcag
    40321 catgatgttg gccggagagt acagagactc cccttcatcc atgatgccct ctttcaccag
    40381 cagttcttca atcagcacca gaccacgttc ggtcaggttc acctggcgag atttttcgtc
    40441 caccgagaag tggccttcgc cctggaaggt ttcggagtct tctttttcct gacggatcag
    40501 gtgcggaata attttattca cgcgtttata catttccgag ctgtcttctg ccgggccgga
    40561 aatgatcagc ggtgtacgcg cttcatcgat caggatggag tccacttcgt ccaccagcgc
    40621 atagtgcagt ttacgctgta cacgttcttc agggctgaac gccatgttgt cgcgcaggta
    40681 gtcaaagccg tattcgttgt tcgtaccgta agtgatgtca gctgcgtaag cttcgcgctt
    40741 tgccggtgct ggcatgcccg gcaggttgat accaacagtc aggccaagga attcaaacag
    40801 cggacggttg ttttcggcgt cacgttgcgc caggtagtcg ttgacggtaa ctacgtgcac
    40861 gcctttaccg gttagtgcgt tcaggtaagc aggcagcgtt gcggtcaggg ttttaccttc
    40921 accggtacgc atttcggcga tgcagcgttc gttaagaacc ataccgccga gtaactgaac
    40981 gtcgaagtga cgcataccaa agacgcgctt acttgcctca cgcaccacgg cgaaagcttc
    41041 cgggatcaga ttttccagca cttcgccttt ttccagacgc gcacgaaact ctgcggtttt
    41101 ccctttcagt tcttcatcgg agagtttttc catctccggt tccatggcat tgatgatgtt
    41161 gaccactttg cgcatccggc gcagggtgcg atcgttacga ctaccgaaaa ctttagttaa
    41221 caatttgatt agcataataa aatctcaaac gccccgcgtt acggagttaa taaaatgaag
    41281 taaaggttta ttgttgttag gtgaggcgtt gagggccagc acggatgcct tgcgcctggc
    41341 ttatccagac gggcgtgctg aattttgctt gtggggtaaa atgcgcataa tcaatgcgat
    41401 aacccttttc agacggcgtg ccttcctggg tcagcagcgc gctgagcgta tccagtaatg
    41461 caagatgttg cgcctgaaga ggcaaagatt cttcagcaac gggcagtgtt tgcggtgcca
    41521 ttgcgaaaga aagatgacgg attaccgtac gaatagcatg ttgatgccag taatcaacgg
    41581 aatagttcgg attcgggcgg cgcgtgttcg cttccagcaa ggccaattga ccaaagttaa
    41641 ctttggctga aggctcgtgg ttgcgggttg tcgcttttgc gggcgcgttt ggttcggcgg
    41701 cgttgctgag cgcaggcaaa cctaaactcg ccgcgaccat ccctaataag agatgcggcc
    41761 agaagtagcg tttaccaaac tgtcgccagc gcgtcagtat tccactcacg ttattgccat
    41821 cccgatcgaa ttcaaacgta tttagcgcac aaatcttatc attaaagcct gaagtccaca
    41881 gcacgaatga agataagttg tgcgtaaaag tgcttaagaa aacagcgttc gcaccgtttt
    41941 ttatagctct tataaaattc aaaaaaaaac gactggttca cctggccgga gagagtgcca
    42001 gatttgccag tcgaatttta tacgacagta taaatgtcgt tatgccagta cagctgaagg
    42061 cgctttgaag gccaacggca gttctgcgtc gtcctggaag gtcacatatt cccaggcttc
    42121 ctgtttcgcc aggacagcct gcagcagttt gttattcagt gcatgaccgg atttataagc
    42181 ggtaaatgca ccaataatat tgtgaccaca catgaacaag tcaccgatcg catcgagcat
    42241 tttgtgacgc acaaattcgt cttcaaaacg caggccgtct tcgttcagta cgcgataatc
    42301 gtcaacaacg atggcacaat cgaagctgcc gcccaggcac aaaccacggg actgcagata
    42361 ttcgatatca cgcatgaaac cgaacgtacg cgcacggctg atctggcgca taaacgcatc
    42421 agcggagaag ttcatcgcat agcgctggtt gctggaatca atagccggat ggttaaaatc
    42481 gatggtgaaa tccagcgaaa aaccattgta cggcttaaat tcagcccact tatcgccatc
    42541 ttcgacacga acagtctctt tgatgcgaac aaattttttg gcgcagttca actcgtcgat
    42601 accggcgtca agcagcaggt atacaaacgg agcggcgctg ccgtccatga tcgggatttc
    42661 cggcgcgtta acttcgataa caatgttatc gatgcccagg cccgcgagag cagcattgag
    42721 gtgctctacg gttgaaatcc gtacatcatg ctcgttgacc agacacgtac agagcatggt
    42781 atcacgcaca gatttggcat cggccgggaa atctaccggt ggattcaagt cggtgcgacg
    42841 atagatgacc ccggtgttgg ccggcgcagg gcgtaacgtc agggtgactt tcttgccggt
    42901 atgtaaaccg acacccgtcg cctgaacgat acgtttaagt gtcctttgtt tgatcatcgt
    42961 attatctcgc caaattacct atccaaccga agtgtactat acattcggcg ggccagttta
    43021 gcacaaagag cctcgaaacc caaattccag tcaattctta atcagcttgc ttacgtagga
    43081 atgctgggat atccagataa tccggctctt tcgcagtttg cggcgcattg tcattcacta
    43141 ctttagcaac cggcttctgc tcctgggtca gcggagccat cccatgctgc tggtagcgat
    43201 ccatcactgg ctgctgaacc tgcttattgg tcaccagagt gatttcagga cgtttgtcca
    43261 tgccgatacc tgtcgcaaca acggttacgc gcagctcgtc attcatatcc gggtcaagag
    43321 aagtaccgat aaccacagtc gcgttgtcgg aagcaaatgc acggatggtg ttacctaccg
    43381 tttcgaactc atccagacgc aggtcgaagc ccgccgtgat gttaaccagc acgccgcgcg
    43441 cgccagacag gtcgatatct tccagcagcg gagaagagat agccatttca gcagcttctt
    43501 ccgcacggtc ttcaccgctc gccacgccag aacccatcat tgcgtagccc atttcagaca
    43561 ttacggtgcg tacgtctgca aagtccacgt tcatcaaacc cggacgagta atcagttcgg
    43621 cgataccttg cacagcgcct ttcagtacat cgttcgctgc gccaaacgca tccagcaggg
    43681 agataccgcg gcccagaact ttcagcagtt tgtcgttcgg gatagtgatc agagagtcca
    43741 catgcttgga cagttcagtg atcccctgct cggcgaatgc catacgcttc ttgccttcaa
    43801 agttgaaagg cttagtgacg acagcaacgg tcaggatacc caaatctttt gccacttcag
    43861 cgacgactgg tgctgcacct gtaccggtac caccacccat acccgcagca ataaagacca
    43921 tgtctgcacc ttccagcgcc gcacgcaatg catcgcgatc ctcatcagcc gcattgcggc
    43981 caacttctgg attagcgcca gcgcccagtc ctttggtgat accgctaccg atttgaatcg
    44041 tctgtccaac cgctgtttta cgcagcgctt gtgcatcggt atttaccgcg aagaattcaa
    44101 caccttcaat acgctcgcgc accatgtgtt caacagcatt accgccgccg ccgccgacgc
    44161 cgatgacttt aatcaccgcg tcattggtaa gttccattgg ttcaaacata gtttctctcc
    44221 gatttgtgcc tgtcgcctga gtccgtaatc atcgtcggcc tcataaaaat taaaactctt
    44281 ttcgcagcca actattgagt cgcttgatcc acgagccaac tgatgctgta acacgttttt
    44341 ctacttcagc ttcaccgtta agatgtgact ctttcccata gtgaagcaat cccaccgccg
    44401 tcgaataata cggctcctga gcataatccg ttaaaccggt aatgttcagc ggcgcgccga
    44461 tacgcacttg cgtatgaaac acgcgctgag cacaggctgc aagaccttcg atctgcgctg
    44521 cgccaccggt taatacaatg cctgccgcca ggtgatgttt aaccccttgt tggcgaagct
    44581 tttcctgcaa ctgcaatatc tcttcgttga ccaggttgag caactcggta tagcgcggct
    44641 cgatcacctc tgccagggtc tgacgttgca gactccgcgg cggacgaccg cctacgctcg
    44701 gcacttccac gctctcatct tttccaacga tggaacccag cgcacaaccg tggcgaactt
    44761 taatcgcttc ggcgtcgctt ggcggcgtgc caaaggcgta agcgatatca ctggtcacga
    44821 cattgccagc ataaggaatt acctttgtgt ggcgcaatgc cccaccggta taaacggcga
    44881 tatccattgt accaccaccg atatcgacga cgcagacacc cagttcacgt tcatcttccg
    44941 tcaataccga ataacttgat gccagtccgg caaatatcag ttggtcaact ttcagcccac
    45001 aacgttcaac cgctttgacg atgtttttcg ccatatcgtt gtgacatgtg atcaggtgca
    45061 cttttgcctg catccgcaca cccgaaagtc ctaccggatt cttgatccct tcctgatagt
    45121 caatcgcata ctcttgcggg atcacatgca gcacacgatg ctcatcgcgc acacgcaccg
    45181 atttcgcggt atggacgacg ttttccacat cttcttgcgt cacttcttct tcagaaatag
    45241 gcaccatacc aatttcattc tggcagctga tgtgcttacc agaaagcgcc agatataccg
    45301 aagagatctg acaatctgcc atcaattctg cctggtcaat ggcgcgttgt acgcacttga
    45361 ccacggattc gaggtcgttc accccgcctt tatccatacc acgtgacggg cagctgccca
    45421 cgccaatgat attgaccata ccgtcgggca gaacttcccc tactaaagcg gcaaccttcg
    45481 cggtaccaat ttccagtcct actaccagtt ttctgtccgt cgccttgatc attgttgttc
    45541 tgcctgtgcc tgattttgtt gctgagtaga ttcctctggc ggcaagggcg cccagcctac
    45601 tgccgctcca gagtcataac gcaaatcaac gtagctaatc cgtttgccat cggtttgcgc
    45661 ctgctgctgt aaaaccggat aaagttctac aaagcgaacc aaacgtttca tcgtatcgcc
    45721 ccggccaaga ttgagcttaa tatcgttatt cagcgtcaac tgccaggaac gccgcgcggt
    45781 catcgccgct tccttcagag taaatctgtc ctttgccagc atctgcccca tttcgcgata
    45841 gccctgcaac acttcattag cgctgccttc cgggccatac agcattggaa gcacctgctt
    45901 gctggtgcga tctggcggca cgctgaaggt gtttccttcc gcgtctacca tgtgttgatc
    45961 attccaccgc gcaatcggca catattcaac cagatgaatc ttcaattcat caggccactg
    46021 ctttctgacg ctcacctgct taatccacgg caggcgttgt tctatttgcg tctggatgat
    46081 gttgacatcc tgggtcataa aggtacccgg ctcacccaat gccaggatcg actgccggat
    46141 atcgtcatta cgcgtgtaat ggcgttcacc ggtcaacacc agctttgaga gcggcaggcg
    46201 ttgcgcatct tccatccagc tcaacacgac ccagccgctc accaacactg tcgttaaaac
    46261 ggtcagcagg aaaaggatcc ccgccagacg cgttccatta ttgcggcgag aagaaacctc
    46321 ttcttcgctg tttcgcgtgt tcagagcagc ctgcgacata ttagtccgcc agttccagaa
    46381 ttcgtactac caactgcgag aagctcatac ctgcctgacg tgccgccatc ggcaccaggc
    46441 tgtggctggt catacccggt gaggtattgg cttccagcag ataaaactgt ccatcactgt
    46501 ccagcataac gtcaatacgt ccccatcctt tgcaacctaa cgtcgtccat gctttcagca
    46561 ctaatgcctg caaattggcc tcttgtgacg cttccagacc tgcggggcag aaatactgtg
    46621 tctcatcaga gagatacttc gcctcataat catagaaggt tccggacggt tgaatacgta
    46681 ttgacggtaa aatttcttca ccgagtatcg caaccgtgaa ctccggccca cttagccatt
    46741 tttcaatcaa tacttcttca tcgtgctgaa atgccaatct taatgcatct tgtagagcat
    46801 tttctgctac tacttttgac attcccacac tggaaccttc gcggctcggc ttaacgataa
    46861 ccggcaaacc cagagcagaa atttctgcta actgcttatc gctcaggcct ttttcaaact
    46921 ctgcgcgggt taacgctacc cacggcgcga ccggtaaacc ggcaccttgc cacagaagtt
    46981 tgctgcgtag tttatctatt gaaagcgcag atgccatcac tccgctgccg gtataaggca
    47041 agcccatcag ctcgagcatg ccctgcagcg taccatcttc accgccgcga ccgtgtagcg
    47101 cgataaacac tttctgaaaa cccatcgact tcagttgcgt cacgtcgact tctttcgggt
    47161 cgacaggata cgcatcaata ccgccttcac gcagcccggc taacaccgct gcgccagaat
    47221 tcagagaaac ttcccgctca gcggaagtcc cgcccaacag gaccgcgatt ttatcagtca
    47281 tgttgttctt cctccggagt ttgcggcttc agtttgattt cagctaaaga acgggcaatt
    47341 tttccgatat taccagcccc ctgaacgaga atcaggtcgt taccggttaa taccggtgcc
    47401 agcatctcgg ctacctgcgc agggtccggc accagaatgg gatcaatttt cccacgtcca
    47461 cgaattgtgc gacacagcga acggctgtcc gctcccggaa ttggcgcttc gccagccgga
    47521 tacacttcca gcatcaacag ggtatcaacc tgcgtcagca cattggcgaa atcatcatac
    47581 aggtcgcgcg tacgggtaaa acggtgcggc tgaaacagca ttaccaggtt tttatccggc
    47641 cagcctgcgc gcgccgcttt aatggtggcg tcaacttccg tcgggtggtg gccgtagtca
    47701 tcgaccagca ttgccgtacc gcttttacca ttcactggct ccagcgggaa ttcaccgagg
    47761 aaatcaaaac ggcgaccagt cccctggaag ctctccagcg cacgcaaaat agcctcgtcg
    47821 tcaattccct cttccgtagc aaccgcaacc gcagctgcgg cgttcagcgc gttatgacgg
    47881 cctggcgcat tcagggtgac gcacatcggc tctttgtcct gacgcagcag cgtaaagtgc
    47941 ccctgcgggc caatctgctg ataatcttct acacgcacgt cggcatcttc gctgaagccg
    48001 taagttgtgg tctgacgtcc cacacgcggt aacaattcgc ggatcaccgg atcatcaaca
    48061 cacatcaccg cacgaccgta aaaaggcagg ttgtgcagaa aattaataaa ggtctgtttt
    48121 aaattctcaa agtcgccctg gtaggtatcc atgtggtcgg cttcgatatt ggtgacaatc
    48181 gccaccatcg gttgcagatg caggaacgat gcatcactct catctgcttc ggcaatcagg
    48241 taccgaccat gccccaaacg cgcatgaacc cccgccgctt ttaccagccc gccgttaacg
    48301 aaggttgggt cgagccccgc ttctgcgtag atgctggaaa ccatcgcggt agttgtcgtt
    48361 ttgccgtgcg ttcctgcaat ggcgatgcca tgacgaaaac gcattaactc agccagcatt
    48421 tcggcacgac ggatcaccgg aatacgcgct tcatgagctg cgacaatttc cgggttgtcg
    48481 gcagaaatgg cgctggaaac aacgaccacg ctggcatcac gtacgttttc cgggcgatgg
    48541 ttgaaataaa tcgtcgcacc cagattcagt aactgctgcg tgaccggatt tggcgctaaa
    48601 tcggaaccac tgatctgata accttcattg gccagaactt cggcaatacc gcccataccg
    48661 gcaccaccaa tgccgacaaa atgtatgtgc cgaacgcgac gcatttcggg cacgatggaa
    48721 cgcagttttg ccaattgttg tgtattcatt ctttacgcca ttaacttctt aaattcatac
    48781 gatgcaaaag gcatcgctac aattacgccc gggcagcccg gctcacttca tttgccactc
    48841 gctcggtggc atccggaatg gatgcagcgc gggcgcgttc tgccatggtt aataaggttt
    48901 ctcgcgacca cccggccagg gtgttggcga cagcatccac gctaagctgt ggctgctcga
    48961 taattttggc tgcgcccgct ttttccagcg gtagcgcatt ccagtattgc tggcggtctt
    49021 tatgttgaaa cggcacaaac aacgccggta gtcctgccgc ggcgatttca ctcacagtta
    49081 aagcaccgga gcggcaaacg acgacatccg cccacgcata cgccgccgcc atatcatcaa
    49141 taaattcagt cactttatgc tgcggttgcc ccgcttcggc atacgcctgt tcaacggatt
    49201 gttgcgaacc tttgccgctc tgatgccaga tagtgactga atcacccagt ttcgcagcaa
    49261 cctgcggcat tgtctggtta agaatgcgtg cgccctggga accacccact accaacacac
    49321 gaaccggacc ttcacgtcca gccaaacgtt gctgcggcaa cggcagcgcc agcacatcgg
    49381 tacgcaccgg gttacccacc acttccgcat tagggaaagc gcctggaaac gcctgcatca
    49441 ctttggtagc aatcttcgcc agccatttat tggttaagcc cgcaataccg ttttgttcat
    49501 gaagtacaac cggaatgcct aacgaccacg cggccagacc acctggacct gatacatagc
    49561 cgcccatacc gagtaccacg tcaggtttgt acgctttcat aatcgcccgc gcctgacgcc
    49621 aggcgttgaa gatacgcagc ggcgcagcta tcagtgcttt tatacctttt ccacgcaaac
    49681 cagagatacg aatgaaatca atttcgatgc catgttttgg cactaagtcc gcttccatac
    49741 ggtcggcagt ccccagccag cgaacttgcc aaccctgagc cattagatag tgcgcaaccg
    49801 ccagtcccgg gaatacatgt ccaccggttc cgcctgccat caccattaat cgctttcctt
    49861 gaccactcat cgtgaacctc gtacaaacgc ctgcgctttc tccagacgcg tttcataatc
    49921 aatacgcaac agcatcatga tggctgtcga cataatcagt aagctcgaac caccgtaact
    49981 gattagcggc aatgtcagac ctttggtcgg taacatcccc gccgccgcgc ctacgttaac
    50041 cagcgcctgg aagctaaacc agatgccaat agaacaggcg agaaaaccgg aaaaacggtg
    50101 gtcaatttct aatgctttgc ggccaatcga catcgcacga aaagcgacga agaataccat
    50161 taaaagtgct agtaccacac cgacataacc taattcttcg ccgataatgg cgaaaataaa
    50221 gtcagtgtgc gcttccggca gatactccag tttttgtacc gaattaccta aaccttgccc
    50281 ccaaagttcg ccgcgaccaa acgccatcag cgattgcgtt aactgatagc cgctgccaaa
    50341 gggatcttcc cacgggttcc agaatgcggt aacacggcgg atacggtacg gttcggcgag
    50401 gatcagcaac acaaccgctg aaatgcccat accgataatg gcaatgaact gccacaattt
    50461 cgctcccgcc aggaacaaca tcgccagcgt agtcacaaac aacaccacca ccgtaccaag
    50521 gtctggctgt gccagcagta acactgccaa caccagaatc acgcccatcg gtttcaggaa
    50581 gccgcgcagg ttattacgta cttcgtcgcc tttacgcacc agatagttgg cgatatagca
    50641 aaacagcgac agttttgtca gctccgcagg ctggatacgc agcaaaccga gatcgatcca
    50701 acgcgatgcc cctttaacag cgctacccac taccagcacg atcatcagca ggatgataga
    50761 tccgagcagc atcgtggcac tgtagcgttg ccagaactcc atcggcagac gcagcgtaat
    50821 gatcgccaga ataaacgcca aaatcagata gacaccatca cgcttcgcga agaagaacgg
    50881 atcgttggtt aagcgttgcc ctatgggcat tgacgccgag gtcaccatga taaagccaat
    50941 cgccgcgagg ccgaaggtca gccacagtaa ggtgcgatcg tacatgatca ggctgtcggt
    51001 atctttttcc cgcgagccca tcacccagcc ctttagcgcc gtggagatcc agaccaggat
    51061 actgaatcct ggcaggcgcg gcattttcag gcgagggaga gataaacgca tcaacctaac
    51121 tccttcgcca gacgggcaaa ctcattgcct cgttgttcaa agttcttgaa ctgatcaagg
    51181 ctggcacagg ccggggagag cagaaccata tcgcccggct gaacacgcgg agccagcaag
    51241 cgcatagcct gttccatagt ttcggtttgt tctgccactt ccgggcgtag cgccgccagc
    51301 tgcgcgccgt cacgaccgaa acaatacaga cgtacgttat cgccattcag gtaacgcacc
    51361 agtgggctaa agtccgccga tttaccatcg ccgcccagca acaaatgcag cgtgccgtct
    51421 acgtgcaggc cattcagcgc cgcttccgta ctgccgacgt tggtcgcttt tgaatcgtta
    51481 atccagcgca caccgttatg ctccagcacc acttcaaagc gatgcggcag accggtgaag
    51541 gtggttaacg ctttcaggct gctggcgcgc ggtaaccctg cggcatctgc cagcgccagc
    51601 gccaccagcg cattggtgta gttatgctgc ccggaaagtt tcatctcttt cacattcagc
    51661 actttctcgc ctttaacccg cagccaggtt tcgccctgct gatgattcag gtgatagtca
    51721 cccatgttga cgccaaagct gacgcagcgt tcatccgcac cgcgaatcgg cattgttaag
    51781 gcatcatcag cattaaccac gcaaactttc gcgttttcgt aaatgcgcag ttttgctgca
    51841 cgatactgtt gtaaaccaaa cgggtagcga tccatatgat cttcagtcac gttcagaatg
    51901 gtcgctgcta ccgcctgtaa gctggaggtg gtttccagct ggaagctcga cagttccagc
    51961 acgtacagtt cacactcatc atccagtagc atcaacgcag gcaggccaat attgccaccc
    52021 acaccaacgt taacccccgc cgctttcgcc atttcaccca ctagcgtggt gaccgtgctt
    52081 ttgccgttag aaccggtaat cgccacaatc ggtgcttgtg cttcgcgaca aaacagctcg
    52141 atatcgccaa cgatttcgat tccggcatca gcggcagcgc ttaaggatgg atgcgccagt
    52201 gcaataccgg gactggcgac aatcagatct gccgccatca gccattcatc attcagactg
    52261 cccgtgtggc gttctacggc ttcgggtaat ttatccaggc caggcggtgt catacgcgta
    52321 tccataacgc gcggcgtcac accgcgagcg aggaaaaagt ccacgcagga aagcccggtg
    52381 aggcccaggc cgataatgac aacattttta ccctggtaat cagccatgat taacgtacct
    52441 tcagcgttgc cagaccaatc aggaccagca tcagcgaaat aatccagaaa cgcacaatga
    52501 cgcgcggttc cggccagcct ttcagttcat agtggtgatg aatcggtgcc atgcggaaaa
    52561 tacgttgtcc gcgcagttta aaggagccga cctgcaggat gacagaaagc gtttctacca
    52621 cgaacacgcc ccccataatc accagcagga attcctgacg tagcagtacg gcgataatgc
    52681 ctaacgcacc acctaacgcc agcgaaccga catcgcccat aaagacctgc gccggatagg
    52741 tgttaaacca caggaagccc agtcctgccc cgactatcgc ggtacagaca ataaccagtt
    52801 ccccggcgtg tcgcagatac ggtatgtgta aatagctggc aaagttcatg ttgccggtcg
    52861 cccacgccac cagcgcaaaa ccaccggcga caaatacggt cggcataatt gccagaccgt
    52921 caagaccatc ggtcaggttt accgcgttgc cagtacccac aatgacgaag taagccagca
    52981 gaatgtagaa cagccccagt tgcggcatca catctttaaa gaatgggacc accagctgcg
    53041 ttgcgggcgt gtctttgccg gcaaggtaca aagcgaaagc aacacccagc gcaatgaccg
    53101 acatccagaa atatttccaa cgagcgatca accctttggt gtctttacgc accactttgc
    53161 gataatcatc aacaaagcca ataacaccgt aacctaccag caccaccaac acgcaccaga
    53221 cgtacggatt ggacgggtaa gcccacagca gcacggagat cacaatcgct gtcaggatca
    53281 taatcccgcc catggtcggc gtaccgcgct tgctgaagtg tgattcagga ccgtcgttac
    53341 gcactacctg accaaaggaa agtttttgca aatgagcaat catgcgcggg cccatccaca
    53401 atgagatgaa cagcgcggtc agcaggctga cgatggcgcg aaacgtcaga taggaaaaga
    53461 cgttaaagcc ggaataatat ttgaccaaat gttcggccag ccaaactaac atgtcccatt
    53521 ctcctgtaaa gcgcgtacta cctcttccat ggcagcacta cgtgaaccct taactaaaat
    53581 cgtaattacc tgttgctcag caatcagtga tttaagacgc gtaattaacg cagttttatc
    53641 agcaaaatgt tcgccaacgc cgctggcggt gctgatagca tggctttgtt tacccatgct
    53701 taacacgcgg tcaataccag cagcttttgc cgcctcaccc acctgtacat ggcaggcttc
    53761 gctttcagcg cccagttccg ccatatcgcc caccaccagc acgcggtagc ccggcatttc
    53821 agccagtacc tgaacagctg cggtcattga accgacattg gcgttgtagg agtcgtcgag
    53881 cagcaactgg ttttctgcca gttggatggg gaacagacgg ccaggaacag ctttcagatt
    53941 tgccagcccc gctttgatag catcaagcgt tgcgcccacg gacatggaga gcgcagcggc
    54001 tgccagcgca ttcgcaatat tgtgacgccc cggcaacggc agcagaacat cgacgctacc
    54061 ggttggggtt tgtagggtaa attccatacc gtgcgaggtc acatggatat tggtggcggt
    54121 gaaatcgctg ttggcggcat tgggtgagaa acgccacact ttgcgtgagc caattacgct
    54181 ctgccagttc agccagtcgt tgttgtcggc gttcataatg gcgataccgt tttccggcag
    54241 gccgctaaag atttcacctt tcgctttcgc gacacccgca agcgagccaa aaccttccag
    54301 atgcgccgct gccaggttgt tgaccagcgc cgcttccggg cgagtcagac tcacagtcca
    54361 ggcaatctcg ccctgatggt tcgcgccaag ttcaataact gcgtaatcgt attccggcgt
    54421 taagcgcaac agcgtcatcg gtacaccgat gtcgttgttg agattgcctg ccgtataaag
    54481 cgtgttgccg cactggctta aaatcgccgc cgtcatctct ttaacggagg ttttgccgga
    54541 ggaccccgtc agagcaacca cgagcgccgg aacttgctgg cgaacccatg cagccagttc
    54601 accaaacgcc agacgcgtat ccttgacgat taactgcggc aggtcgatgt ccagcggacg
    54661 gctaaccagt agtgcgcctg cgccgccagc tttcgcctgg tcggcaaaat cgtgggcatc
    54721 aaaacgttca cctttcaggg caacaaacag gcagcccggc gtcagttttc gggtatcagt
    54781 ggttacagca tcaagggtga tatctgcacc ttgcagttca ccgttgagaa tatcggtaag
    54841 ttggctaagg gttacgctaa tcatgcaatc ccccccagca gacgcgccac cgtgacgcga
    54901 tcggagtagt ccagacgctg attgccaaca atctggtaat cctcatggcc tttgcccgcg
    54961 accagtacca catcattctc tttagcctgc ataacggcgc aagtcaccgc ttcagcacgg
    55021 ccttccatca ctttggcatg tccggcatct aacattcccg ccagaatatc gttgatgatg
    55081 gcacgcggtt cttcggtacg cgggttatcg tccgtcacca ccgccacgtc agcaaactct
    55141 tcggcaattg cgcccatcag tggacgctta cctttatcgc gatcgccacc acagccaaag
    55201 acgcaccaca gcttgcccgc acagtgcagg cgcgccgcct gtaaggcttt ttccagtgca
    55261 tccggcgtat gcgcgtaatc caccaccacc gtcggtttgc ctggcgcagt gaacacttcc
    55321 atacgtccgc aaaccggttg cagacgcgcg gcggttttca gcagatcagc cagtggatag
    55381 cccagcgcca gcagtgttgc cagcgcgagc aacaggttgc tgacgttaaa agcgcccatc
    55441 agatggcttt caatttcgcc atcgccccag cttgaactaa agcgaatggt cgcgccgctg
    55501 tcgtgatagt tcacttcggt cgctttcaac cagcgtccgt gacagttcgg attgatatga
    55561 tcttccattg ataccgcaac cgcgtccggc agttttgcca gccagcggcg gcccacttca
    55621 tcgtcggcgt taataatcgc ctgaccgcaa tgatgttcag aataaagcag ccatttcgca
    55681 gcttcgtagt gttccatatc accatgataa tcaaggtgat cgcggcttaa gttggtaaag
    55741 accgacgccg gaaatttcaa tgccgccaca cggtgctgta ccagcccgtg ggaggaaact
    55801 tccattgcgc aaaaagtcgc gccctgatcc accagccccg ccagctcatg ctgaacatca
    55861 actgccgaac cggtagtatt ttctgtcggg atcactttcc ccagcagacc gttaccaacg
    55921 gtgcccatta ccgcgctggt ttcgccaagc agttggctcc actgcgccag aagctgggtc
    55981 gtcgtggttt tgccgttggt gcccgttacg cctacgagac gtaaattgtc agagggttca
    56041 tggtaaaagc ggcccgccag tgcagataaa cgctcgttga gctggctgag atagatgacc
    56101 ggtacgccgt gcatttcacg gatttcaccg tcggtcgcct catctttcgc ctctgcaata
    56161 atggcagcca caccttgcgc tatcgcctgc gggatatatc gacgcccgtc cgcctgatga
    56221 cctactacag ctacaaagag atcgcccgcc gcagccacac ggctgtcgag tgtcatctct
    56281 cgcagtgctc gcgaaggtgc gtctggcacc cacggagcaa gaaggtcgcg caaattacga
    56341 tctgccacct gtcccctcgc cttgattaat cacaaattca tttttatcgc ccgttgtcag
    56401 cgcatccggc tcgatgttca tggtacgcaa tacgccgccc atgatggcac caaagaccgg
    56461 cgcggaaacg gcgccgccgt agtatttacc cgcctgcgga tcgttgataa caacaaccag
    56521 cgcgaagcgc ggctgactcg caggcgcaac gcctgcggta taagcaatat atttattgat
    56581 gtagcgaccg tccggcccga cctttttcgc ggtaccggtt ttaatggcga tacgatagcc
    56641 tttaatcgcc gccttcacgc cgccgccgcc tggtagcgcc acgctttcca tcatatgcac
    56701 cacggtgcga acaatggatt ccgggaagac acgttcaccg ggaaccgggg ggtcaacttt
    56761 ggtaatcgac agtgggcgat aaatgccgta gctgccgata gttgcgtaga ctcgcgctaa
    56821 ctgtaacggt gttaccatta gcccgtagcc gaaagagaag gtggccctct ctatgtcaga
    56881 ccaccgttgt ttttgaggat ataagccact gcgttctccg accaacccca aattggtcgc
    56941 ttttcccagt ccaaaacgtg agtaagtatc tactaacgct gaggacggca tcgttaacgc
    57001 cagcttggaa acaccgacgt tactcgactt ctgtaatacc ccggtcaggg ttaattcgct
    57061 gtagcgtgcc acgtctttga tttcgtggcc gttaattcga taaggaatgg tattgagtac
    57121 cgagttttcc cgcaccacgc cacgttgcaa cgcggtcatc accaccatcg gtttaaccgt
    57181 tgagcccggt tcaaacacgt cggtgatggt acggttacgc atcgcctctt tcggcgtgcc
    57241 gctcagattg ttagggttgt atgacgggct gttagccatc gccagcactt cacctgtgtt
    57301 gacatccacc agcacggcgc taccagattc agccttgtta aaggccaccg cgttgttcag
    57361 ttcgcgataa accagcgcct gcaggcgttc atcaatactc agcgccaggt tgtgcgctgc
    57421 ctggctgtca gtagaagaaa tatcttcaat tacgcgacca tagcggtctt tacgcacaat
    57481 gcgctcaccc ggctgcccgg taagccattt atcgaaactc ttctcaacgc cctcaatccc
    57541 ttgactatcg acgttagtaa agccgatgag gtgagcagtc acttcgccgg acggatagta
    57601 acggcgagac tcttcacgca gatgaatccc cggcagtttc agttttttga tgtagtccgc
    57661 catgtcagag tttacctgac gcgccagata aataaagcgc cctttcgggt tggcgttaat
    57721 gcgggcagaa agctgatcca gcggaatatt gagcgcgtta gccagcgcct tccagcggtc
    57781 accgacgctg ataccgccag cgtcatgcac ttctttcggg tcagcccaaa tcgcttttac
    57841 cggcacgctc accgctaacg ggcgaccaga acggtcagta atcatgccgc gggaggtgga
    57901 aacttgctga acgcgaagag aacgcatgtc gccctctttc accagcatat ccggggagat
    57961 aacttgtaac cacgctacgc gtccgagcag aaaagccagc gccaggagaa tacagccgca
    58021 taacaacgca aaacgccaac tgataaagtt ggcatgttct tcctgacgtt ttggtttctg
    58081 cgttttcgcc gctgctttca tgcgtcgcgt ttatccttat ttttgcacta cgatattttc
    58141 ttgtgacgga tcaacatgct gcatttgcag cttttccgtg gcgatccttt ccacccggct
    58201 atggtcgccg agcgcattct cttcaaggat caggttgcgc cattcaatgt ctaaagcatc
    58261 tcgctccagc accagttgtt cgcgctgagc ggtcagtaaa cgggtatggt gcgccgtggt
    58321 taccacggtc accgccgtca aaataatgca aatgaacagg cagagtggca gcttcccaaa
    58381 tcgcaaaaga tcgtcaccga taacaccagg caatgcatgg cgctcgtggc ttcccatcga
    58441 tcctttaact ttgcttagag cttctgtcac tctgctgatc atgcgttcgt cctctctgca
    58501 atacgcagaa ctgaactacg ggcacgaggg ttctcagcca cctcttcttc gcccggcatt
    58561 aacttgccta gtgctcgcag ctgacggcca cccagttttt tgagctgctc ttcagtcatc
    58621 ggtaaccctg ccggaacttg cggaccgcgg ctgttttcac gcataaaacg tttcacaata
    58681 cggtcttcca gcgagtggaa gctgatgatc gaaagccgcc cacccggggc cagcacgttg
    58741 agcgagcttt ttagcgcctg ctctatctcc tccagttcac tgtttaccca aatgcgcacc
    58801 gcctggaagg tacgggtcgc gggatgttta aacttatctt tcaccggcgt tgcagcagcc
    58861 acgacttccg ccagttcttt ggtgcgggtc atcggctgtt cgcggttacg ctcgacaatg
    58921 gcgcgggcaa tgcgtttggc aaaatgctct tcaccatagg ttttcaatac ccaggcgata
    58981 tcggcttctt ctgcggtttg tagccattca gcggctgact gcccacgggt tgggtccata
    59041 cgcatgtcca gcggaccatc gcgcataaag gaaaagccgc gttcagcatc atcaagttgc
    59101 ggtgaagaga cgccaagatc gaggagaatg ccgtcgatct tgccaataag atcgcgctcg
    59161 gcaacgtatt cgcccagcgc ggagaaaggt ccgtggatga tggagaagcg cggatcatca
    59221 atagtcttcg caacggcgat agcctgcggg tcgcgatcga tcgccagcaa acgcccctct
    59281 tcgccaagct gcgagaggat cagacgtgag tgaccaccgc gaccaaaagt cccatcaatg
    59341 tagatgccat caggacggat attgaggcca ttaacggctt catccagcag caccgtagta
    59401 tgtttatagt tttccatcat tttatagaga caagtcctgc agtcgctccg ataagtctcc
    59461 ggtagccaac tgctctgcgt cgatatcttc cttgacctgt tgatgccagg ttgtttcatc
    59521 ccacagctca aacttgttga actgtccaac cagcatcact tcttttgtca gcccggcatg
    59581 ttgccgcagt actggcgcga ttaacaatcg acctgcgcca tccatctgac attcgctggc
    59641 atgacctaac agtaggcgct gcacacggcg ctcaaccggg ttcatgctca acagacgcga
    59701 taatttttgc tcgataattt cccattcagg cagggggtaa agcagcaggc acgggtgatg
    59761 aatgtcaatg gtgcaaacca tttgaccggc agcgttctca agcagctgtt cccgataacg
    59821 ggtaggcact gataagcgcc ctttgctgtc gagattgact aacgttgctc cccggaacat
    59881 gccagcctca ccccttattc ccactttgcc ccacaatttc ccactgaaag gagtttacgg
    59941 agcggaggaa aagcttgtca agccagtcac aaggcatacc gaggtaaaaa acccgatgtt
    60001 tacggctaat atcagcgttg tttattttac gtccaacgtg cgaagcaaat taacgttatg
    60061 aatagctgta agaaaaaatc cctcatcgtc atcactgttt taaaatgact cactattttc
    60121 cacagcaaat ataaagtgtc agtttgcgac gcgagcggca ttttaggaca tatcttcccc
    60181 ggttaacagt ccttgttacg tctgtgtggc gcttgatgcc agcgggtttg cgcaaaagca
    60241 aagaaaagtg tttgttaaat cggcaattca tcgccgcatg taacaaaata atgcaaaaac
    60301 gcgctgtcga tatcacttaa ttattgtgaa tttcagctgg tgagttaatt aacgggcggg
    60361 tttaaaacca tttatcaata atagtcagga atagtcttat ttactttaag gatattgat
//
LOCUS       DAZPCS010000006        58718 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZPCS010000006 DAZPCS010000000
VERSION     DAZPCS010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58718
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.006"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(33..2480)
                     /gene="glgP"
                     /locus_tag="ACHUQ7_000326"
     CDS             complement(33..2480)
                     /gene="glgP"
                     /locus_tag="ACHUQ7_000326"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFR1133908.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2499..3932)
                     /gene="glgA"
                     /locus_tag="ACHUQ7_000327"
     CDS             complement(2499..3932)
                     /gene="glgA"
                     /locus_tag="ACHUQ7_000327"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFR1133909.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHVHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KSQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3932..5227)
                     /gene="glgC"
                     /locus_tag="ACHUQ7_000328"
     CDS             complement(3932..5227)
                     /gene="glgC"
                     /locus_tag="ACHUQ7_000328"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFR1133910.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5245..7218)
                     /gene="glgX"
                     /locus_tag="ACHUQ7_000329"
     CDS             complement(5245..7218)
                     /gene="glgX"
                     /locus_tag="ACHUQ7_000329"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFR1133911.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7215..9401)
                     /gene="glgB"
                     /locus_tag="ACHUQ7_000330"
     CDS             complement(7215..9401)
                     /gene="glgB"
                     /locus_tag="ACHUQ7_000330"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFR1133912.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SIHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9674..10777)
                     /gene="asd"
                     /locus_tag="ACHUQ7_000331"
     CDS             complement(9674..10777)
                     /gene="asd"
                     /locus_tag="ACHUQ7_000331"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFR1133913.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10969..11562
                     /gene="yhgN"
                     /locus_tag="ACHUQ7_000332"
     CDS             10969..11562
                     /gene="yhgN"
                     /locus_tag="ACHUQ7_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFR1133914.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11619..12959)
                     /gene="gntU"
                     /locus_tag="ACHUQ7_000333"
     CDS             complement(11619..12959)
                     /gene="gntU"
                     /locus_tag="ACHUQ7_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFR1133915.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGAATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12963..13490)
                     /gene="gntK"
                     /locus_tag="ACHUQ7_000334"
     CDS             complement(12963..13490)
                     /gene="gntK"
                     /locus_tag="ACHUQ7_000334"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFR1133916.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIACSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13629..14624)
                     /gene="gntR"
                     /locus_tag="ACHUQ7_000335"
     CDS             complement(13629..14624)
                     /gene="gntR"
                     /locus_tag="ACHUQ7_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFR1133917.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14848..15543)
                     /gene="yhhW"
                     /locus_tag="ACHUQ7_000336"
     CDS             complement(14848..15543)
                     /gene="yhhW"
                     /locus_tag="ACHUQ7_000336"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFR1133918.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSCANYYDPNFMGFSALRVIN
                     DDVIEVGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15666..16703)
                     /locus_tag="ACHUQ7_000337"
     CDS             complement(15666..16703)
                     /locus_tag="ACHUQ7_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFR1133919.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17036..17524
                     /gene="yhhY"
                     /locus_tag="ACHUQ7_000338"
     CDS             17036..17524
                     /gene="yhhY"
                     /locus_tag="ACHUQ7_000338"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFR1133920.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(17583..19325)
                     /gene="ggt"
                     /locus_tag="ACHUQ7_000339"
     CDS             complement(17583..19325)
                     /gene="ggt"
                     /locus_tag="ACHUQ7_000339"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFR1133921.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDIKKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            19445..19885
                     /locus_tag="ACHUQ7_000340"
     CDS             19445..19885
                     /locus_tag="ACHUQ7_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFR1133922.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNSDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19872..20615)
                     /gene="ugpQ"
                     /locus_tag="ACHUQ7_000341"
     CDS             complement(19872..20615)
                     /gene="ugpQ"
                     /locus_tag="ACHUQ7_000341"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFR1133923.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QHAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20612..21545)
                     /locus_tag="ACHUQ7_000342"
                     /pseudo
     CDS             complement(20612..21545)
                     /locus_tag="ACHUQ7_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
     gene            complement(21604..22449)
                     /gene="ugpE"
                     /locus_tag="ACHUQ7_000343"
     CDS             complement(21604..22449)
                     /gene="ugpE"
                     /locus_tag="ACHUQ7_000343"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFR1133924.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22446..23333)
                     /gene="ugpA"
                     /locus_tag="ACHUQ7_000344"
     CDS             complement(22446..23333)
                     /gene="ugpA"
                     /locus_tag="ACHUQ7_000344"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFR1133925.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSMVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(23431..24747)
                     /gene="ugpB"
                     /locus_tag="ACHUQ7_000345"
     CDS             complement(23431..24747)
                     /gene="ugpB"
                     /locus_tag="ACHUQ7_000345"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HFR1133926.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAVFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTSVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(25144..25857)
                     /gene="livF"
                     /locus_tag="ACHUQ7_000346"
     CDS             complement(25144..25857)
                     /gene="livF"
                     /locus_tag="ACHUQ7_000346"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFR1133927.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(25859..26626)
                     /gene="livG"
                     /locus_tag="ACHUQ7_000347"
     CDS             complement(25859..26626)
                     /gene="livG"
                     /locus_tag="ACHUQ7_000347"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFR1133928.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(26623..27900)
                     /gene="livM"
                     /locus_tag="ACHUQ7_000348"
     CDS             complement(26623..27900)
                     /gene="livM"
                     /locus_tag="ACHUQ7_000348"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFR1133929.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(27897..28823)
                     /gene="livH"
                     /locus_tag="ACHUQ7_000349"
     CDS             complement(27897..28823)
                     /gene="livH"
                     /locus_tag="ACHUQ7_000349"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFR1133930.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(28871..29980)
                     /gene="livK"
                     /locus_tag="ACHUQ7_000350"
     CDS             complement(28871..29980)
                     /gene="livK"
                     /locus_tag="ACHUQ7_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFR1133931.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINVKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            30404..30787
                     /gene="panM"
                     /locus_tag="ACHUQ7_000351"
     CDS             30404..30787
                     /gene="panM"
                     /locus_tag="ACHUQ7_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFR1133932.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(30986..32089)
                     /gene="livJ"
                     /locus_tag="ACHUQ7_000352"
     CDS             complement(30986..32089)
                     /gene="livJ"
                     /locus_tag="ACHUQ7_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFR1133933.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(32360..33214)
                     /gene="rpoH"
                     /locus_tag="ACHUQ7_000353"
     CDS             complement(32360..33214)
                     /gene="rpoH"
                     /locus_tag="ACHUQ7_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFR1133934.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(33459..34517)
                     /gene="ftsX"
                     /locus_tag="ACHUQ7_000354"
     CDS             complement(33459..34517)
                     /gene="ftsX"
                     /locus_tag="ACHUQ7_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFR1133935.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(34510..35178)
                     /gene="ftsE"
                     /locus_tag="ACHUQ7_000355"
     CDS             complement(34510..35178)
                     /gene="ftsE"
                     /locus_tag="ACHUQ7_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFR1133936.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(35181..36677)
                     /gene="ftsY"
                     /locus_tag="ACHUQ7_000356"
     CDS             complement(35181..36677)
                     /gene="ftsY"
                     /locus_tag="ACHUQ7_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFR1133937.1"
                     /translation="MAKEKRRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSSEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVAEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            36827..37423
                     /gene="rsmD"
                     /locus_tag="ACHUQ7_000357"
     CDS             36827..37423
                     /gene="rsmD"
                     /locus_tag="ACHUQ7_000357"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFR1133938.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            37413..37682
                     /locus_tag="ACHUQ7_000358"
     CDS             37413..37682
                     /locus_tag="ACHUQ7_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFR1133939.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(37685..38044)
                     /locus_tag="ACHUQ7_000359"
     CDS             complement(37685..38044)
                     /locus_tag="ACHUQ7_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFR1133940.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            38185..38811
                     /locus_tag="ACHUQ7_000360"
     CDS             38185..38811
                     /locus_tag="ACHUQ7_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFR1133941.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            38885..41083
                     /gene="zntA"
                     /locus_tag="ACHUQ7_000361"
     CDS             38885..41083
                     /gene="zntA"
                     /locus_tag="ACHUQ7_000361"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFR1133942.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAT
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(41185..41430)
                     /gene="tusA"
                     /locus_tag="ACHUQ7_000362"
     CDS             complement(41185..41430)
                     /gene="tusA"
                     /locus_tag="ACHUQ7_000362"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFR1133943.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            41651..42316
                     /locus_tag="ACHUQ7_000363"
     CDS             41651..42316
                     /locus_tag="ACHUQ7_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFR1133944.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            42389..42946
                     /gene="dcrB"
                     /locus_tag="ACHUQ7_000364"
     CDS             42389..42946
                     /gene="dcrB"
                     /locus_tag="ACHUQ7_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFR1133945.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(42950..44200)
                     /locus_tag="ACHUQ7_000365"
     CDS             complement(42950..44200)
                     /locus_tag="ACHUQ7_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312349.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1133946.1"
                     /translation="MKHCCKNVVILMPEPVAEPALNGLRLNLRIVSIVMFNFASYLTI
                     GLPLAVLPGYVHDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLC
                     GCFLSGLGYLTAGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIG
                     RVISWNGIVTYGAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASK
                     GKPLPFRAVLGRVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFV
                     GTRLLFPNGINRIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFP
                     ALGVVAVKAVPQQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVA
                     IALLLTWRLKKRPPEYVPEAASSS"
     gene            44301..45350
                     /locus_tag="ACHUQ7_000366"
     CDS             44301..45350
                     /locus_tag="ACHUQ7_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFR1133947.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            45405..45992
                     /gene="acpT"
                     /locus_tag="ACHUQ7_000367"
     CDS             45405..45992
                     /gene="acpT"
                     /locus_tag="ACHUQ7_000367"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFR1133948.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRTLLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRVNWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            46103..47677
                     /gene="nikA"
                     /locus_tag="ACHUQ7_000368"
     CDS             46103..47677
                     /gene="nikA"
                     /locus_tag="ACHUQ7_000368"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR1133949.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALNKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            47677..48621
                     /gene="nikB"
                     /locus_tag="ACHUQ7_000369"
     CDS             47677..48621
                     /gene="nikB"
                     /locus_tag="ACHUQ7_000369"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFR1133950.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGSWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            48618..49451
                     /gene="nikC"
                     /locus_tag="ACHUQ7_000370"
     CDS             48618..49451
                     /gene="nikC"
                     /locus_tag="ACHUQ7_000370"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFR1133951.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWQPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGH
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            49451..50215
                     /gene="nikD"
                     /locus_tag="ACHUQ7_000371"
     CDS             49451..50215
                     /gene="nikD"
                     /locus_tag="ACHUQ7_000371"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFR1133952.1"
                     /translation="MPQQIELRNITLQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPYALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            50212..51018
                     /gene="nikE"
                     /locus_tag="ACHUQ7_000372"
     CDS             50212..51018
                     /gene="nikE"
                     /locus_tag="ACHUQ7_000372"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFR1133953.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWHGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIAETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            51024..51425
                     /gene="nikR"
                     /locus_tag="ACHUQ7_000373"
     CDS             51024..51425
                     /gene="nikR"
                     /locus_tag="ACHUQ7_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFR1133954.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(51545..51904)
                     /locus_tag="ACHUQ7_000374"
     CDS             complement(51545..51904)
                     /locus_tag="ACHUQ7_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFR1133955.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(51901..52176)
                     /locus_tag="ACHUQ7_000375"
     CDS             complement(51901..52176)
                     /locus_tag="ACHUQ7_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFR1133956.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVCDWLLSIGVKP"
     gene            complement(52235..53359)
                     /locus_tag="ACHUQ7_000376"
     CDS             complement(52235..53359)
                     /locus_tag="ACHUQ7_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1133957.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(53359..56094)
                     /gene="rbbA"
                     /locus_tag="ACHUQ7_000377"
     CDS             complement(53359..56094)
                     /gene="rbbA"
                     /locus_tag="ACHUQ7_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFR1133958.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIQARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREARINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(56091..57158)
                     /locus_tag="ACHUQ7_000378"
     CDS             complement(56091..57158)
                     /locus_tag="ACHUQ7_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFR1133959.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(57221..57334)
                     /gene="yhiY"
                     /locus_tag="ACHUQ7_000379"
     CDS             complement(57221..57334)
                     /gene="yhiY"
                     /locus_tag="ACHUQ7_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFR1133960.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            complement(57524..>58718)
                     /locus_tag="ACHUQ7_000380"
     CDS             complement(57524..>58718)
                     /locus_tag="ACHUQ7_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFR1133961.1"
                     /translation="ANDVAVTESYKPIWNWEISQYNVFDIKFETIIKPQFADRTYFSN
                     CSPVDPTSTRPTYFGDTDGSVGAVLYALFATGHLGIMAEGENFLSQLLNIEDEVLNVL
                     LRENFNEQLDTNVNTIISILNRRDNVLESLQPYLVINKDAVTPCTFLGDQTGDRFSNI
                     CGDQFIIDLLKRIMSINENVHVLAGNHETNCNGNYMQNFTRMKPLDEDTYDGIKDYPV
                     CFYDPKYKIMANHHGITFDDQRKRYIIGPITVSIDEMTNALDPVELAEIINKKHHAII
                     NGKKFKTSRAISCRSFNRYFSVSTDYRPKLEALLACSQMLGINQVVAHNGNGGRERIG
                     ETGTVLGLNARDSKHAGRMFSMHNCQINPGAGPEITTPWKSYQHEKNRNGLMPLIRRR
                     TMLQL"
BASE COUNT        13804 a        16271 c        14963 g        13680 t
ORIGIN      
        1 accctttggc cccgttctat ttattagtga aattacaatc tcaccggatc gatatgccag
       61 atatgatcag cgtactcttt gatagtacgg tcagaagaga agtagcccat attggcaatg
      121 ttcagcatcg cttttgcggt ccactcttcc tgaagctcgt agagttcgtc gactttatcc
      181 tgacaatcga catagctgcg ataatccgcc agtacctggt agtgatcgcc gaagttgatc
      241 agcgaatcaa ccagatcgcg atagcgaccc ggatcttccg gactgaatac accgctgccg
      301 atttgcgtca gcacctgatg cagctcctca tctttctcgt agtattcacg cggtttgtag
      361 ccctgacgac gcagttcttc cacttcttcc gctgtgttac caaaaataaa gatattgtca
      421 gcaccgacat gatccagcat ctcgacattc gcaccgtcca acgtaccgat agtcagcgca
      481 ccgttaagcg caaacttcat gttactggtg ccggaagctt ccgtccctgc cagcgaaatc
      541 tgttcagaca gatctgccgc cggaatgatc aactgcgcca ggctaacgct gtagttcggg
      601 atgaacacga ctttcagctt atcgccaatc tgcggatcgt tgttgatcac tttcgctacg
      661 tcattgatca aatgaataat gtgcttcgcc atgtaatagg ccgaagccgc cttaccgcca
      721 aaaatattca cgcgcggtac ccacttcgca tccgggtcgg ccttgatgcg gttatagcgg
      781 gtaatcacat gcaacacatt catcaattga cgtttgtatt cgtgaatacg tttgatttgt
      841 acatcgaaca acgcctttgg attcaccacc acattcagct gctgggcgat atactctgcc
      901 agacgctttt tgttctccag cttcgcctga tgcacagcgt gattaaccat tgggaaatca
      961 cagtgttgtt gcagctcatt aagcaggcta aggtcggtgc gccagttacg gcccaggtgt
     1021 tcgtccagca cggctgaaag cgatgggttc gctaccgcca gccagcgacg cggcgtcaca
     1081 ccgttggtga cgttggtgaa acgacccggg aagattttcg caaagtcggc aaacaacgat
     1141 tgcaccatca gattagagtg cagttccgat acaccgttaa ctttgtggct cacaacaacc
     1201 gccagccagg ccatacgcac acgacgaccg ttggattcat caatgatcga cgcccgtccc
     1261 agcagatcgg tatcgttcgg atactgttcc tgcaaggttt tcaggaaata gtcgttgatt
     1321 tcaaagatga tctgcaggtg acgcggcaga attttaccca gcatatcaac cggccaggtt
     1381 tccagcgcct cgctcatcag cgtgtggtta gtgtaggaga agacctgaca acacacctca
     1441 aacgcgtcgt cccagctaaa ttggtgctca tcgatcagca gacgcatcat ctcaggaatc
     1501 gacagtaccg gatgggtatc attgagatga atcgcgattt tatccgccag gttatcgtag
     1561 gttttatgca actgataatg acggcttaaa atgtcctgaa tggtcgagga aaccaagaag
     1621 tattcctgac gcaggcgcag ctcacgcccg gagtaggtgg agtcatccgg atacagtacg
     1681 cgagatacgt tctcggagtg gtttttatct tccactgccg cgaagtagtc accctggttg
     1741 aatttaccga ggttaatttc gctactggct tgcgcactcc acaaacgcag cgtgttggtc
     1801 gcgtcggtgt cgtaaccagg gattatctga tcgtaagcga ctcccagaat ctcttcggtt
     1861 tcaatccagc gcgttttttt accttcctgc tgaatgcgac caccaaaacg gactttatag
     1921 cgcgtgttgt ggcgtttgaa ttcccacggg ttaccgtatt ccagccagta gtctggcgac
     1981 tctttctggc taccgttaac gatgttctgc ttgaacatac cgtagtcata gcggatgccg
     2041 taaccgcgcc ccggcaaccc taacgtcgcc agagaatcaa ggaagcaagc cgccagacgt
     2101 cccaggccac cgttaccgag gcctgggtca ttttcttcat caatcagctc ttcgagattt
     2161 aaccccatcg cttccagtgc gccctgtaca tcttcgtaaa ttcctagcga caacatggcg
     2221 ttggagagcg tacggccaat caaaaactcc atcgacaggt agtaaacctg acgagtttct
     2281 tgcgacaact gggcacggtt tgaacgtaac cagcgctcca cgagacgatc gcgcacagca
     2341 aataacgttg cgttcagcca ttcatgttta ttggcgacga ccgggtcctt tccaatcgta
     2401 aacatcagct tgtaagcgat agagtgctta agagcttcta cgctaagcgt gggcgatgaa
     2461 tatgtaaacg gagcattcat ataggcgttt cctgaaaact atttcaagcg atagtaaagc
     2521 tcacggtacg acttcgccgc gacctgccag ctaaaatcca ttgccatagc ctgacgttgc
     2581 acaaaccgcc acagtgaagg acgggaccac agtacaaaag cacgccgaat agcccgtaac
     2641 agcgaccagg cattactatc ttcaaagaca aacccactgg cgacgccatc tgcaaggttc
     2701 tcaagagaac agtcagaaac cgtatcagca agcccaccgg tgcgccgcac taacggcagc
     2761 gtaccgtact tcaatccata aagttgcgtt aagccgcacg gttcaaaacg gctgggcacc
     2821 agaatgacgt ccgcgccgcc cataatgcga tgcgaaaatg cttcgtgata gccaatctga
     2881 acgcccacct gaccggggta ttccgctgcc gccgcaagga aaccttcctg cagcaccgga
     2941 tcgcccgcgc cgagtagcgc cagctgcccg ccctgctcca gaagccccgg taaggcttcc
     3001 agcaccaggt cgagaccttt ctggctggtc agacggctca ccactgcaaa aagcggcact
     3061 ttatcgtcaa ccttaagccc cattgcgatt tgtaactggc tcttattttc cgctttatct
     3121 tccaacgtat cgcgggtgta acgcgaggcc aacagtaagt ccgtctctgg actccagatt
     3181 ttctcgtcca cgccgttcag tacgcctgaa agacgccctt cacggtgacg ctgttgcaac
     3241 agaccttcca taccgtaggc aaactgcggt tcggtgatct cgcgagcgta ggttggactg
     3301 accgccgtaa tgtgatcggc atagtacaga ccggccttca ggaaagagat ttgtccgttg
     3361 aattccagcc catgaatatt aaagaatgac catggcaatt ggatgtcatt catgtgatgt
     3421 gcataaaaca tgccttgata ggccaggttg tgcacagtaa acaccgactt cgcaggacgc
     3481 ccgcgcgccg ccagatacgc aggcgcaaga cctgcatgcc agtcgtgcac atgcaccaca
     3541 tcaggacgcc agaatgggtc aagcccgctg gccatttctg ccccaaccca ccccagcagc
     3601 gcaaaacgca atacgttgtc ggtataggca aataagttgg tatcgtgata cgggcttccc
     3661 ggacgatcat agagatgcgg cgcgtcaatc aggtatatgc caaccccgtt gtaatgaccg
     3721 aacaacagcg tgatatgccc ggcgaaagta tcacgacggg atactacctg cgcatcggtc
     3781 acgccacggc gaatatcggg aaatgcaggc aacagtacgc gagcgtcaac gccgtctgcg
     3841 atttgtgctg cgggtaatgc cccaataaca tcagccagac cgccggtttt aagcagcggg
     3901 aacatctctg aacatacatg taaaacctgc attatcgctc ctgtttatgc cctaacttcc
     3961 gtagcatttc gcgcgttacc agcacgatgc cttcttctga acgatagaaa cgacgtgcat
     4021 cttcctctgc gttttcacca atcaccatgc cttccgggat aacacaagca cgatcgatga
     4081 cgcagcggcg cagacggcac gagcgaccta cccatacttc cggtaacaat acggcggaat
     4141 caatgttgca gaatgaattc acgcgaacgc gcgagaacag aacggactgc accaccaccg
     4201 aaccggagat cacacaaccg ccggatacca gtgagttaag ggtcatcccg tggctaccgg
     4261 agcgatcctg cacgaatttc gctggcggta atgattcatt gtaggtgcga attggccaat
     4321 tgcgatcgta catatccagt tccggcacca cagaggccag atcgagattc gctttccagt
     4381 aagcttccag cgtacccaca tcgcgccagt acggctcggc atccgggtcg gattgtacgc
     4441 aagagagcgg gaacgggtgc gcataggcca gaccggcttc ggtgatcttg ggaatcaaat
     4501 ctttgccaaa gtcgtggctg gagttctcat cacgatcgtc ttcttccagc agttcataca
     4561 gatagtcggc gtcaaagacg tagataccca tactcgccag agatttgctc ggatcgttcg
     4621 gcattgacgg tgggttagca ggtttttcaa cgaattcgat aattttatcg ttctcatcaa
     4681 ccgccataac gccaaatgcg gaggcttctt caatcggtac tggcatacaa gcaacggtgc
     4741 aacgtgcgcc tttttcgacg tgatcgataa gcatacgcga gtagtcttgc ttgtagatat
     4801 ggtcgcccgc caggatcacc acgtattccg ctttataacg gcggataatg tcgaggtttt
     4861 gggtgaccgc atctgcggtg ccgcgatacc agttttcccc tttcattctc tgctgtgctg
     4921 gcagcagatc gacaaactcg ttcatttctt cattgaagaa tgaccagccg cgctgaatgt
     4981 gctgcaccag agtgtgggac tggtactggg tgatcacgcc catacgacgg atcccagagt
     5041 tgatgcagtt agacagcgca aagtcaataa tgcggaactt accgccgaag tgtacggccg
     5101 gttttgctcg cttattggtt aaatccttca ggcgggtacc acgtcctccc gccagtatca
     5161 gggcaacaga tttcaatggc agctggcgcg ccaacattaa gtgatcgttc ttctctaaac
     5221 taaccatgac taactccttt tttatcatct ctggaacaca cacaatccgt gtgcaggtcc
     5281 ctgccagaca accgtaatca ctgggttatc ctctccagcg aatgggggaa tggcgtgcca
     5341 ctccccagca ggtaaaacaa tctctgttac ctcaagcgtg gcgttaattg cgatcaaaaa
     5401 gcgatccgag agcagaattt gcagctgttt cggcccgttt tgccactcat ccgtgcttaa
     5461 aggttgagca tatcgattta gccaacggac attgccgtca ccttcttccc accagcgatt
     5521 ctccaccaaa gcgggaatgc gcttgcgcag atggattaac gcggcggtaa atgcggttaa
     5581 accactgctt gcctgcgacc agtccaacca gtttaattgg ttatcctgac agtaggcatt
     5641 gttattgcca tgctggctgt gaccatgttc gtcaccggcc agtaacatcg gcgtgccctg
     5701 ggagagcaac aacgttgtta acagggcatg aatgctgtcg cgccgccgtt caaccaggtc
     5761 aagagtaccg cctaaccctt ctttaccatg attgttactg taattgttgt tggtcccgtc
     5821 gcgattttct tctccgtttg cttcattgtg tttatggttg aagcaaacgc agtcgcgaag
     5881 cgtaaaaccg tcatgcgcgg tgacgagatt aatcgcggca ctcggcagac gaccattacg
     5941 tttaaaaaca tcgctggagg cagcaaaacg cccggcaaac gcccccagag gcaaatcata
     6001 atgtagccag aaacgacggg cagcatcgcg gaaatgatcg ttccactcgg caaacagcgg
     6061 cgggaaattt cccacctgat aaccactagg agcgatatcc cacggttcag caattaactt
     6121 cacctgcgag agcaccgggc agttctggat agcggtaaac aacggcgcat cctgacggaa
     6181 ctctggcgta cggcccatga ctgccgccag atcaaagcgg aaaccatcga cgtggcaggt
     6241 ttctacccaa taacgcaggc aggcgctggc ataatccacc accgccggat gactcaaatt
     6301 gagcgtgttg ccgcaaccgg tccagttgtg ataatcgccg tcttctctta tccaataata
     6361 gctacggtta tcgatcccac gcagcgagaa taacgggccg tcgaggtcca gttccgcact
     6421 atggttgagc acgatatcaa gaatgacttc gatacccgct ttatgcagtg ctttgattgc
     6481 atcgcgaaac tcatccagcg ccgtttctgg cgagcaggca tacgccggat gcagcgcaaa
     6541 catcgccacc gggttgtaac cccagtagtt acttagcccc atgcgttgca gacgtggttc
     6601 actggcaaac tgcgccactg gcagcagttc cagcgcggta atgcccaatt gtttcaaata
     6661 gttgatcatc accggatgcc cgagggcttt ataagtgcca cggatctcga ccgggatctc
     6721 cgggtgcaag tacgttaatc ctttgacatg ggcttcataa atgatggtgc tgccccacgg
     6781 cgtgcgcggc ggggcgtcat cttcccagtc atagtgatca accaccacta cgcatttcgg
     6841 cgcaatggcg gcgttgtcgc gatagtcagg ttcattatga ccggcgtgca gcagcgggtt
     6901 atctttaaac tccccgtcaa tttgccgcgc gcaaggatca atcaacaact tcgccgggtt
     6961 aaagcgatgc ccctcggcgg gttgccaggg gccatgaacg cgataaccat aacgcaaacc
     7021 cgggcgcgca tccggcagat aaccgtgcca aatgtcgcca ctgttccctg gcaagtcata
     7081 gcgatgttcc tggccattgg cgtcaaagac acacagttct acccgctcgg catgagcgga
     7141 gaaaagtgtg aagttgacgc cctgaccgtc gtaatgcgcg ccgaggggag cgggtttgcc
     7201 aatggcgagt tgtgtcattc tgcctcccga accagccaga tagtggccag cggtggtagc
     7261 gtcaggctta gtgaatgctg acgaccgtgg ctggcaatct catcgctgtg taccgtgccg
     7321 ccattgcctg cattactgcc gtgatagtgt atggaatcgg tattgaggat ttcacgccat
     7381 ttacctggct gattaatgcc gaagcgataa tcatgacgcg gcaccggcgt aaagttactg
     7441 gcaacgataa tttcgttacc ctctttatcg cgacgcacaa agatcagcac cgagcgttct
     7501 ttgtcatcca ccaccagcca ttcaaagccg tacgggtcaa aatccagttc atgcattgct
     7561 ttatggtggc ggtaggtgag gttcagatcg cgcaccagac gctggacacc gtggtgccag
     7621 ttatcgccgc cttccaacag atgccagtcg aggctggcgt catggttcca ctcgcggccc
     7681 tgggcaaatt cgttccccat gaacagcagt ttcttgcccg gaaatgccca catccagcca
     7741 tagtaagcgc gcaggttagc gaatttctgc catgcgtcgc ccggcatgcg gtcgagaatc
     7801 gattttttac cgtggaccac ttcatcatgc gacaacggca ggacgaagtt ttcagtgtag
     7861 ttgtagagca tcccgaaggt cagtttatcg tgatgatact gacgataaat cgggtcgagc
     7921 ttcatgtagt ccagggtgtc atgcatccag ccgaggttcc acttgtacca gaagcccaga
     7981 ccgcccatat cctgcggacg agaaacgcca gggaaatcgg tagactcctc agccattgtc
     8041 accgcaccgg aaacctgctc accaagaata cggttggtat tacgcaagaa ttcaatcgct
     8101 tcaagattct cgcgcccgcc aaattcgttc gggatccact ccccctcttt acggctgtag
     8161 tcacgataaa tcatcgacgc caccgcatcg acgcgcagcg catcaatacc aaaacgttca
     8221 atccagtaaa gcgcgttacc gacgaggaag ttactgactt cacggcgacc atagttgtag
     8281 atcagcgtgt tccagtcctg atgatagcct tcacgcggat cgctgtgttc atacaagttc
     8341 gtgccatcaa attcggcaag cgcaaagtca tcagtcggga agtggcctgg cacccagtcg
     8401 agaatcacgt tcagaccagc tgcgtgtgcg gcatcaatga aataacggaa gtcgtcgcga
     8461 gtaccaaaac ggcgggttgg tgcatacagg ccggttggct gataacccca actgccatcg
     8521 aagggatgct cgttaatggg cagtagttcg aggtgggtaa agcccatcca tttagcataa
     8581 ggcaccagtt gatcggccag ctcgcggtag ctcaaccaga aattgttgtc ggtgtggcga
     8641 cgccaggaac ccaggtgaac ttcataaata gagattggcg catcaaactg attcgctttt
     8701 ttgcgctctt cagtctgtac aaccttttcc ggcagcccgc aaataagaga cgcggtttcc
     8761 gggcgcattt gcgcttcgaa ggcataaggg tcggacttca gacgcaagtt gccattggca
     8821 tcaatcatct cgtatttata gagctgaccg ttatgcgccc cagggataaa cagttcccag
     8881 atgccgctct ctttacgcag gcgcatcggg tgacggcgac cgtcccagta gttgaattgc
     8941 ccaaccaccg agacccgacg ggcgtttgga gcccagacag agaaacgcgt acctgtgacg
     9001 ccatccatag tatctgcatg cgcgcctaag gtttcatacg ggcgcaggtg agtaccttca
     9061 gataatagcc aggcatccat ttcctggatt agcggaccaa aacggtaagg atcatcaatc
     9121 aggttttgct gaccatgcca gacaacagcc aactgatagc ggaaaaaatt cttacgtcgc
     9181 ggaatgacgc cgctaaagaa tccccgtgag tcgagacact ccagttttgc gagtttgcgc
     9241 ccggttttcg gttcaatcac ccacacatcg gtagcgtcgg gtaaaagggc acggacttcc
     9301 agtcccgcgg tggttttatg cattcccagt acggaaaaag gatccgcaaa atggcctgca
     9361 attagcgcgt taatcacgtc tctatcgata cgatcggaca tgcttgtctt cctgttttat
     9421 tgtgtcaccc catccaattg gatttttgac ttctggttgt gacatttttt tgacctgaac
     9481 ggcgcagcac tctgtgcatc ctctctgcgt cgtcctcact tcaggtaagg ctgtgaatac
     9541 tcatgtattc agccaccctt aaagaatagc caatgctcta tttaactccc ggtaaatcat
     9601 gaaacatctg cgcttactcc tgtattacgc actaacaggg gcggcatcgc gccccagatt
     9661 taatgaataa agattacgcc agttgacgaa gcatccgacg cagcggctcc gcggcccccc
     9721 acagcagctg gtcgcccacg gtaaaggctg acaggaactc tggtcccata ttcagcttac
     9781 gcaggcggcc taccggcgtg gtcagcgtgc cggtaacggc agctggggtt agctcacgca
     9841 tagtgatttc ccgatcgttc ggaacgactt tcgcccacgg attgtgcgca gccagcagtt
     9901 cttcgacggt cggaatagac acatcttttt tcaatttaat agtgaatgcc tggctgtggc
     9961 agcgcaatgc cccgacacgc acacataaac catctaccgg aattacggaa gatgtgttga
    10021 ggatcttgtt ggtttccgcc tgccctttcc actcttcgcg gctctgaccg ttatcgagct
    10081 gtttgtcgat ccacggaatc aggctacccg ccagcggcac gccaaagtta tccaccggca
    10141 gctcaccgct acgggttaag gttgtgactt tgcgttcgat atcgagaata gcagaggacg
    10201 gggtcgcgag ttcatctgcc acatggccat acagatggcc catctgggtt aataactcac
    10261 gcatatgtcg cgcaccaccg ccggaagcgg cctggtaggt tgcaacggac acccaatcaa
    10321 caagatcatt ggcgaataaa ccacccaacg acatcaacat caggcttacg gtacagttac
    10381 cgccaacaaa agtcctgatg ccattattta atccgtcggt aatgacgtcc tgattgacgg
    10441 ggtcaagaat gatgatggcg tcatctttca tgcgcagaga cgatgctgcg tcaatccagt
    10501 aaccttgcca tccgctttca cgaagctttg gatagatttc gttggtataa tcgccgccct
    10561 gacaggtcac aatgatatcg agggccttta gcgcctctag atcaaaggca tcctgaagtg
    10621 tgccagtggt tccgccaaaa gacggcgcag cctggccaag ctgagaagta gaaaagaaga
    10681 cagggcgaat ggcgtcgaag tcgcgctctt caaccatgcg ttgcatgaga acggagccga
    10741 ccataccgcg ccagccgata aaaccaacat ttttcataag cgttttttcc tgcaaagatg
    10801 tgtgctgtat aaatgtgccg gtctcctctt ggcacatctt tcaccataca aaaagctgcc
    10861 aaagtcgcaa gtgaaattaa tcaatgatag cgaagccatc agtaatgcga cttatcctgc
    10921 tttgttagca cgcagaaagt ccgcggcaat tatcagggaa tttgagttat gaatgaaatc
    10981 atttctgcag cagttttatt gatcctgatt atggatccgc tcggaaacct gcctattttc
    11041 atgtccgtac tgaaacatac tgaaccgaaa agacggcggg caatcatggt gcgagagttg
    11101 cttattgctc tcctggtgat gctggtgttc ctgtttgcgg gtgagaaaat tctggcattt
    11161 ctcagcctgc gagcagaaac cgtctccatt tctggcggca tcattctgtt tctaatcgcc
    11221 attaagatga ttttccccag cgcttcagga aatagcagcg ggcttccggc aggtgaagag
    11281 ccatttatcg tgccgttggc aattccgtta gtcgccgggc cgactattct cgccacgctg
    11341 atgttgttgt ctcatcagta cccgaatcag atgggacatc tggtgattgc tctgctgctg
    11401 gcctggggcg gcacctttgt catcctgcta cagtcttcgc tatttttacg tctgctgggc
    11461 gagaaagggg tgaacgcact tgaacgcctg atgggattga ttctggtgat gatggcaact
    11521 cagatgttcc tcgacggcat tcgaatgtgg atgaaggggt aacgaggaga gtttttcgaa
    11581 aagagcccgg tagcgtcaca actaccgggc gaacaaactt aactcaacag ctggaacgca
    11641 atcatcccaa cgatggcacc aacagtgccg aggatggttt ccatcatggt ccaggttttc
    11701 agcgtttcgg cttcggtcgc gccggtaaat ttaccgaaca accagaaacc agcgtcgtta
    11761 acgtggctga caacaatcga accaccagcg atacaaatcg acagtgccgc catttgcgca
    11821 ccagagtagt tcagttgttc aatgactggc atcaccagtc ccaccgccgt taaacaggct
    11881 acggtggcag ccccctgaat gatgcgcact gcagcagcca gcacgaagca ggtgatagca
    11941 atcggcaggc ccatgccggt taacgcttcg cccagtgccg gacctacgcc agagtcaacc
    12001 agcacctgtt tgaacacgcc gcctgcacca atcaccagca gaatgatccc cgccggttgc
    12061 agcgcgtgac cgcaaatctc catcactttg tcttttggca tgccctgacg cattgccagg
    12121 ccataaatcg ctaccagaca agcaaccaga atcgcggtaa acggatgacc aataaactcg
    12181 aaccattcgt aagcggtaga gccttctggc acaaaacgcg cggcaatggt tttcagcccc
    12241 accagcacca gcggcaacag gatcagcgac aggctgaatc cgaaagatgg cattttacct
    12301 tcgccgagat gcggttcgct gatgtcgtca ggaatatgca gctcaacgta acggctgatg
    12361 aagttacccc acagcggccc ggcaataatc attcccggaa ttgccgcaca caggccaatc
    12421 aggatcatcc agccaaagtc ggcattcatc tgcgatgcca gcagcattgg cgctggccca
    12481 ggcaccagga acgctgccgc ggccgccacg cctgcgaata atgggattac cagcttcacc
    12541 aggttcgtac cggtgtggcg cgccattgag aaagcaacgc taatcagcag aacaatcgcc
    12601 acttcaaaga acagcggtag cgcacagacc agccccgcaa ggccgatggc ataatgcgcg
    12661 cggctgtgac cgaaggattt gagcattttg acggcaatct gatcgactgc gccggtttca
    12721 tataagatct tgccaaacat agctcccagg gcgacaacca ccgccaggaa gccgagggtg
    12781 cctcccatcc ctttttccat cgtcgctgcg attttatcga gcggcatacc ggaaaaaagg
    12841 ccagccccca tggacaccac catcaaagcc aggaaagcgt gcatacgcgc cttcatgact
    12901 aaaaacagca gcagtaaaac agaccctact gctgttaaaa caagcgttaa tgtagtcact
    12961 acttatttgc cttttttaat aacctcaatg gtgcttgcca caacaccttc cagcggttga
    13021 tcgatatcca ccaccagtac atcggtttcg tccgcacccg gctcctgcag cgtttcaaac
    13081 tgcgtcacca acatttgggt tttaaagaaa tggcctttgc gcgctttcag gcgactttca
    13141 atcacatcaa aatcgccttt caggtagatg aaagagagat tcggattacc ttcacgcagc
    13201 aagtcgcgat agtgcttttt cagtgcagaa caggcgatca gcgacacttt attggtgcgc
    13261 tgcatagcaa acgcggagtc gttcagcgcc tgcaaccagg gtttgcgatc gtcgtcattc
    13321 agcggttcgc cagacgccat tttttcgata ttgcgccgtg gatggaggaa atcgccatca
    13381 agaaacgcgg catgaagttg atgcgccact tcactggcga ccgcagattt gccgctgccc
    13441 gatacgccca tcaagacgta aatgtggtga tcatggttag tcgtgctcaa agtggtgccc
    13501 ccacaataca agaattaaaa atgttacggg taactgttat cggcaacatt gtccagccgg
    13561 acaatatcag aagcaatatc catccgtgcc ttaagtgtat aagtgtgagc tacttcaaat
    13621 ttgtgggctt aaatagatcc gcccggtgac aaggtgaaac ctaaatctaa cattttcggt
    13681 gtcacagatt cgccacgaat acgcgccagc aggcgttcag cgccaatact gcccatccgc
    13741 tcacgcggcg tcagcacgct cgcaagtcgt ggctccatca cctgaccaat gtcatgaccg
    13801 tggaaaccgg caatcgccat atcgtcagga acttttaacc ccagacgctg acattcaaac
    13861 gccgcgccga ccgccagatc atcattagta cagaacacgc catccagctg cggatattcc
    13921 cgccgcgcct ggcgaatcag ttcaataccg gaagagtaag aagaagattg ctcaaccatc
    13981 acgctatatg gcaccaggcc tgcatccagc atcgcctgtt cgtatccctt ctgtttgatg
    14041 atagtacgtt cgtcgagacg tgcgccgaga taggcaatgt ggcgatgccc acgagcaata
    14101 atggcggtag tcatctggcg tgccgcttca aagttatcaa aaccgacggc gatatcgagg
    14161 cacggcgact ggctgtccat caactccacc accgggatcc ccgccacttc aatcatcttt
    14221 aaggtgcgcg gcgtgtgggt acgttcggtg aggatcaggc cgtcgatatt ccaggagagc
    14281 atggattcga gacgttcttg ctccatttcc ggtttataac cgtagtgcgc cagcatggtc
    14341 tgataaccgt gcgcgtcggt gacgctttcg attccgcgta atacttccgc gaaaacctgg
    14401 ttggtgagag aaggtaacag gacgccaatc gcccggctgg tagcgttaga gaggatatcg
    14461 ggcgcacgat tgggaatata gcccagttca tcaagagcgg cggcaatctt gccgcgtaga
    14521 gcgacggaaa cctgctccgg gttgcgtaaa aaacggctga ccgtcatttt ggtcacgcct
    14581 acacggtcag ccacatcctg aagtacgggt cttttctttt tcatcgtcct gaaggtacaa
    14641 aagagataga ttctcttagt ttaacacgga cgcatcagaa ccttcccggg gaaaacagtg
    14701 cttgtttaca attattagat atacactaaa tcaattgcat cgcattgtgc taacgacgcg
    14761 gcaaaacccc ggaagcgtgc atcagcacgc gaccggagcg agcgaggaag cctgcaaaaa
    14821 ggcaggcttc aaagatgccg ttgagtttta aaccggcggc agatcgaaca gtaacacttc
    14881 gctgtcgcta tccgcatgga tggagattgc ctgctcatcc cagattgcca gaccatcgct
    14941 ggtcgaggct ttcacgccgt taatggtaac attgcctttt accacctgaa tccagacgcg
    15001 gcgttcagcg gcaatctgat gcaccgactg ctcatctttc agcaacgccc aacgatacag
    15061 ttccatatcc tgatgcactt tcaacgaacc atctcgcgca tccggcgaga gcaccagctg
    15121 tttgccctgc acggcatcga agcgacgctg ttcataacgc ggcgtaatgc cattttcttc
    15181 aggcatgatc cagatctgat acagatgcag acgctcggtg ctgcttgggt tgtactctga
    15241 gtgacgaata cccgtacccg cactcataat ctggaactca cccgccggaa cctgctcttt
    15301 attgcccatg ctgtcctgat gctcaacagt accttccagc acgtaggtca aaatttccat
    15361 atctttatgc gggtgagtgc cgaagccctg ccctacttca atcacgtcgt cgttaatcac
    15421 gcgcagcgcg gagaagccca taaagttcgg atcgtaatag ttggcacaag agaaagtatg
    15481 ccaggagtcc agccagccat gatttgcatg accacgttca tttgctttgc gtaagtagat
    15541 catttgtatt cgccccctga atgatttcga tgggcttagt gtggacccga tctgcctggg
    15601 atgatagagg gtgaaaattg ccccctctgt tcaaaaaaat tgaactattc gaggggccat
    15661 caaacttaat tcgcgagagt cactgtggag ggagaggctt gctcaaatcc gcgttcaagg
    15721 atttccagat tggtaagaac ttcagattcc ttgacgtaat ttggcgcacc gtgggtgatg
    15781 gtttgataca acgcatcata aacacgcccg taatcgccca tctccggctt catctcttct
    15841 ctgaccgtca caccctcgtc attgacatac tccagcacac cgaccgaatc atccgctgcg
    15901 aatcccggtt cgcccggcat aatattagcc ttcaggctgg tttcctgctg gtcgataccg
    15961 tatttaataa acgaaccttt cttaccgtga acgataaatt tcggataatc gattttcacc
    16021 agatggctgg ttttgacgat ggctttcagg tcgccataaa acagttgcgc ttcaaaggtg
    16081 tcgtcaggat tggctttatt acgcaggctg cggatgtcat aagcgacgtg atccgggcga
    16141 ccgaacagag aaataatctg gtccatcgta tgcacaccaa ggccatagaa cgcgccatcc
    16201 tgcggcagtc caggtttggt ttctgccacc gggcggtaat agtcaaaatg gctttccact
    16261 tcgacaatct ctcccagctt gccgctttca atcgcttttt tcgccgtcag gaagcaggaa
    16321 tcaaagcgac gattctgata tggcgtgacg gtcagccctt tgcttttcgc cagtgcaaac
    16381 agctctttcg cctgcgcaag tgtcggagtg aacgtttttt cgaccagcac atttttcccg
    16441 gcttccagcg cgcgcttcgc gtactcgaag tggctgtccg catgggtgca gacaacaacc
    16501 aacttaacat cgggatcgtt tagcacttcg tcgagatcgc tggtgaaatg gatatgagag
    16561 taaatgggag cctgttcttc cggcttcgca tggcgacgaa aaatatgcgc gacatgccag
    16621 ctatccttgc ggttaagtac atacggcaga tggtaacggg tggtgctttt gccgaagcca
    16681 ataaaggcgc agttgatgac catgatttcg tcctttttaa ggtggttatt tacaccttag
    16741 cgcaaagcgg acgtggttcc tactggagtg cgcataacga acacaagcac tcccgtggat
    16801 aaattgagaa cgaaagatca aaaaaaaagc cagcacccgg ctggctaagt aatactggaa
    16861 gcaatgtgag caatgtcgtg ctttcaggtt ctccgcgagg gtcttcctga tcgcgataca
    16921 ataataatca ttctcattcg cacttgtcca acactttttg caaaaaaatg catttgactc
    16981 gcatttgaaa gtcaatgatg ttgaaaggga catttacccc aaagaggaca aaggaatgag
    17041 tgagatagta atacgccacg cagaaacacg ggattacgag gccatcaggc agattcacgc
    17101 ccagccggag gtgtattgca acacactaca ggtgcctcat ccttccgatc atatgtggca
    17161 ggagcgactc gccgatcgtc ccggcatcaa gcaactcgtc gcctgtattg atggagacgt
    17221 cgtgggccat ctcaccattg acgtgcaaca gcgcccacgc cgcagtcatg ttgccgattt
    17281 tggtatctgt gtcgactctc gttggaagaa ccgcggcgtc gccagcgccc tgatgcgaga
    17341 gatgattgaa atgtgcgaca actggttacg ggtagatcgc attgaactaa ccgtgtttgt
    17401 cgataacgcg ccagcaatta aggtctataa aaaattcggc tttgagattg aagggaccgg
    17461 taagaagtac gcattgcgta atggtgaata tgtcgatgcg tattatatgg cgcgggtgaa
    17521 atagagaatt tgcaatctaa gtttgtcatt taagccctca cctaccggga gggcttaaga
    17581 tattaatacc ccgccgttaa atcatccacc gagcgcgggt cggatgcgcc gtacaactca
    17641 ccgtccggcc caaccataat gctttgtgtg ctgcccatcg cctctttcag cgccactttc
    17701 tgaccttttg cttccagcag cttgagcgta tccgggctaa accctttttc gacacgcagc
    17761 tcgtccggca accactgatg gtggaaacgc ggcgcattgg tcgcttcggc gacgttcatg
    17821 ccataatcga tgctattcac caccatttgc agcaccgtgg tgataatccg gctaccgcct
    17881 gggctaccgg taaccagcca ggttttaccg tctttcacca caatggtcgg cgacatcgac
    17941 gacagcgggc gtttgttcgg cccgacggcg ttggcatcac cgcccaccag cccgtaaacg
    18001 ttcggtacgc ccggtttggc ggagaaatca tccatctggt tattaagcag aataccgctc
    18061 tcacccgcga caatacccgt accgaaggtg gtgttcagcg tataggtcac cgccaccgcg
    18121 ttaccgtctt tatccactac agagtaatgg gtagtttgat tactctcata aggcgcaagc
    18181 ttgccagggc gaatctcact ggacggcttc gcttttttga tatcaatttg atcggcaatg
    18241 gatttggcat aggctttatt ggtcagtgcc tgccacggga ctttgacaaa atccgggtcg
    18301 ccaagatatt ccgagcggtc ggcgtaggcg tatttctccg cttctgccat gatttgcatc
    18361 gcatcggcgc tgccaaagcc gtatttcttc atatcgaagt tttccagaat attgaggatt
    18421 tgtacgatat ggatcccgcc ggaggatggc ggtggcatgg agtaaacctg atacccgcga
    18481 taatcgccgc ttatcggagt gcgttcgacc gctttatagg ccgctaaatc ttctttagtg
    18541 atcaagccac cgtttttctg catctcctgg gcgatctgtt ccgcaatcgt gcctttatag
    18601 aattcgtccg ggccgttttc agcaatcatc tccaggctct ttgccaggtt cgcctgcacc
    18661 agcgtgtcgc cctttttcag cggctcgccc tctttccaga agatagcttt actgttttcg
    18721 tgattcggca acacttcgct accgtaggtt ttgagatcgt cagccagcgc gtcgttaacg
    18781 ataaaaccat cgcgtgccag tttaaacgcg ggctgcacga ctttgttcag cggcatggtg
    18841 ccgtatttat ccagcgccag cgagaaacct gctaccgtac ccggtgtgcc ggaagccaga
    18901 tgcgaagtga gtgatttttt gctgtccggg ttgccctgat catcgaggaa catatcgcgg
    18961 gtcgctttgg cgggtgccat ttcgcggaaa tcgatagccg tggtattgcc atttttcgag
    19021 cggattaaca taaaaccacc accgcccaga ttccctgcct gcggatgcgt taccgccagc
    19081 gcgtagccca ccgccacggc ggcatcaacg gcattcccgc cctccttgag gatatccacc
    19141 cccacctgag tggcagtggc gtccacagac gctaccattc cctgtttcgc gcgtaccgga
    19201 tggaagacat cttcctccac accatacgag acgggcggcg caggaggcgc ggcggcggcg
    19261 ctaaaacaac ttcctgagag cagagcagca atggccaccc ggcgtaaaaa cgtcggtttt
    19321 atcatcgtta ttctccagag attaaggggc aaccccagct aagcctggta tataactctg
    19381 aattaatcat cgttttgtcg ggaagcgagt aaacttaaag gatatcttca gaggaggata
    19441 cgcgatgaaa cgacttctga ttcttacggc actcctgccg tttgtcggct ttgcacagcc
    19501 cattaatact ctgaacaacc ctaaccagcc ggggtatcag atacccagcc agcagcggat
    19561 gcaaacccag atgcagactc agcaaatcca gcaaaaaggg atgctgaatc agcaactgaa
    19621 aacgcaaact cagttgcaac agcagcattt agaaaaccag ataaacaata attctcagcg
    19681 ggtgttgcag tcacagccgg gggagcgaaa tcccgcccgg cagcaaatgc tgcccaacac
    19741 caacggcggg atgttaaaca gcaaccgtaa tccggatagt tcgttgaatc agcagcatat
    19801 gctgccggag aggagaaaca gcgacatgct gaatcagccc agcacgccgc agcctgacat
    19861 tccgttgaaa actattgggc cgtaaagttc ggaccaatca cgtcaatcgc atcggtacag
    19921 atgcaatcca cgccccagcg cagcaactct gccgcgtgct ggggtttatt gacggtataa
    19981 accagaatcc gtaatccggc gtctttcaac tgcatcactc gcgctttatc gagtaactta
    20041 tgattgagat gaatagagac gcagcccagc cgcgcggtca gttcgcgcca gtcgtcgcgc
    20101 cactcatcaa gcaataaacc acgcggcagt tccggtgccg cctgttgtgc agcttctaaa
    20161 gcatcaatct caaacgatga cagcagcggc ggcgtcatac cggcccacag ttggcgtgcc
    20221 gccagcgcca ccattttgcc cgttaatggt ccagtgccag tggtgggttt gatttcgata
    20281 ttcgccatca tcccgtgttc gcgacagcgt tccgccacct gcgaaagcaa cggcagcggc
    20341 tcacctttaa acgctttgct gtaccaactg cccgcatcca cgcgcagtaa atcctgccag
    20401 ttcagttcac ccgcgacgcc ccagccgttg ctggtgcgtt cgagattgtc gtcatggagc
    20461 aggaagatct cgccatcttt cgataacttc gcgtcaaatt cgatcatctt atgaccgtat
    20521 tttgccccga cgtcgattgc cgccagggtg ttttccgggg ccagcttacc gccgccacga
    20581 tgagcgacga tgcggggata aggccagtta ctcatactcg ttgtcctgtt tcaccatcaa
    20641 aaagatgcag ctgattttcc gccagatgca gccacagcgt gctgcctgcc gtcgggcgct
    20701 cctgatgcgc cagtcgcacc accagcttct gcttgcgagc ttagcgcaat atgttccggg
    20761 cgaatgccga gggtcatttt acgcccggca tactgacggt agccaccgtt tagcggtagc
    20821 acgataccgc cgtccagttc gaaatgcgtg ccttcattat tcatgcggcc tgtcagcagg
    20881 ttcatcgccg ggctgccgat aaaactcgcc acaaacaggc tggcgggctt ttcgtagact
    20941 tccaccggcg tgccaatctg ttcggcaacg ccgccgttca tcaccattac tcgctgggcg
    21001 agcgtcatcg cctcaacctg atcgtgagta acgtagagtg aagtcgtttt caggcgacgg
    21061 tgcagctgtt gcagttcaag acgcatctgt acgcgcagct tggcatcgag gttagagagt
    21121 ggctcatcaa acaagaacac cgccggatcg cgcacaatcg cgcgtcccat cgccacacgc
    21181 tggcgctgac cgccggaaag ctcgcgcggg cgacgtttga gcagaccgtc cagctccaga
    21241 atgcgcgccg cttctttaac gcgctcggct atttgctgct tgcccatgcc gcgaattttc
    21301 agcccccacg ccatgttttc ttcgacgctc atatgcggat aaagcgcgta attctggaac
    21361 accatcgcaa tcccgcgatc tttcggctcc atttcggtca cgcgctggtc gttgatccag
    21421 atatcgcctt cggtcaccct ctccagcccg gcaaccatac gcagcagcgt cgatttcccg
    21481 cagccagacg gcccgaccat cacgataaat tcgccatccg ccacatcaag ggtcagcggt
    21541 ttaatgacct aggttttgcc atcccagctt ttggttactg cctgtaattt cagtcctgcc
    21601 atcttatttc tcactatcga ccaggccgcg cacgaaggca cgctgcatca ctaaaacaat
    21661 cactactggt gggatcaggg ttaacaacat cgccgccatc actgagttcc attccgtggt
    21721 gccttcgcca gtggcgatca tccctttgat ccctgccacg gtggtgccga gatccacgtc
    21781 ggtaataatc aacaacggcc acaaatactg gttccagccg taaataaagg tgatcacaaa
    21841 cagcgccgcc agattagttt tggagagcgg aaaaacgatg tcgcaaaaga agcgcattgg
    21901 tgacgcaccg tcgatccgcg ccgcttccac cagttcatcc ggcagcgtca taaagaactg
    21961 gcgaaacaga aacgtggcgg tcgccgaggc catcagcggc agcgttaaac cggcgtagct
    22021 gtcgagcatc ttcaggttgg cgatgacttc caccgtcggg aagatacgta cttcaaccgg
    22081 cagcatcagg gtgataaaaa tcatccagaa gaagaggtta cgtagcggaa aacgaaacca
    22141 gacaatggca aatgccgaga gcatcgagac agtaattttg ccgagcgtaa tgctgaacgc
    22201 catcacaaag ctgttaagca acatccgcca gaacggcgcg ctatttgtgc ctaccccgtt
    22261 cacccagatg ttgtggatgt tttccagcag atgcgtgccg gggatgagcg tcatcggcgc
    22321 ggcgtagacg gcctgtttat ccagcgtcgc cgcgacaaac gccacgtaca gcgggaagag
    22381 gatcaccgcg atcccgagga tcagcatggt atggctgaat atcgtcagcc acggacggtt
    22441 ctcaatcatt ggtaacgcac cttgctttca acatagcgga actgcaccac cgtcagcacg
    22501 atgacgagga acatcagcac catcgactgt gcggcagacg aagccagatc cagtccggta
    22561 aaaccttcgc ggtagatctt ataaatcagc gtcgtggtgg cctgaaccgg cccgccggac
    22621 gtggcggcgt cgatcaccgg gaaggtgtcg aagaaggcat acaccaggtt cactaccagc
    22681 aggaagaaac tcaccggggc gataagcggc agcgcaatct taaagaagcg gcgaatcggc
    22741 cctgcaccgt cgatggctgc ggcttcgatc aacgaacggg gaatggattg cagcgcggca
    22801 tagaagaaca gaaagttgta gctgatttgc ttccacaccg aggcaaacac caccagaaac
    22861 atcgcctgac cgctattttg cgcgtggttc cagtcgtagc cgaactcagc gagaaaatgg
    22921 gtgatcagcc cgcgaccggg gttaaacagg aagatccaca atacggcggc aacggcggga
    22981 gccacggcat acggcagcaa cattaaggtt tggtagaaac gactgccgcg cacgatgtac
    23041 tccaccagcg ccgcaaagaa cagcgatacc agcaaaccgc tgacggtgac aaaggtgctg
    23101 aattttatcg tcgtccagaa ggagtcgaga taatagctgt catgaaacag cgtgacgaag
    23161 ttatccagac cgacaaactg gctggagaaa ccaaacggat cgacgctttg tagcgagtac
    23221 cacaacgctt cgcccgcagg ccagataaaa aagataacgg tgatgatgag ctgcggcgcg
    23281 accagcagat aaggcagcca gcgcgagcgg aacaccggac gggatgatga cattacggtt
    23341 aattcctgaa ctgtgccgga tgcgcttcgc ttatccggcc tacacgcgat gccaggtcag
    23401 ataagcgaaa cgcatccggc attacactga ttaagacttc gtcgatttct caaagcggcg
    23461 cagtaactgg ttcccacgct caactgcggt atccagcgcc tgctgtggcg tcttcttacc
    23521 ggtccacacg ctctcaagct cttcatccac aatcacgcgg atctgcggca tgttgcccag
    23581 acgaagccct ttggtgaacg gcaacggcgg cttgttcagc atctgacgcg tcgcggtatc
    23641 cgcacctggg tttttctcgt aaaagccctg ctcacgggtc aggtcatacg ccgctttggt
    23701 aattggcaga taaccggttt tctgatgcca ctcagcagcg ttttctggct tcgcgaggaa
    23761 gtcgagaaac ttcgccacgc cggtatacgt ttctttatct ttgccctgca tcacccacag
    23821 gctggctccg ccgataatgg cgttttgtgg cgcatctttc gcatcggcgt cgtaaggcat
    23881 catacctacg ccgtagttaa atttggcgta ctcgcgaatg ttggcaagag aaccggaaga
    23941 ggcggtggtc atcgcgcaat caccgttata gaacttctcg gtggattcat ccttacgacc
    24001 gacgtagctg aagtcgccct tcttgttcat ctcctcgagc atggcgatgt gtttcacctg
    24061 ctccggcttg ttgaactcca gcaccgcgtc cgtgccgtca aagccgttgt ttttgctggc
    24121 aaacggcaga ccgttccagg cgctaaagtt ttccagttgg atccagccct gccagccgct
    24181 ggcgtagccg cacttaatac cggaggcttt cagttttgcg gaatagtccg ccagatcctg
    24241 ccaggttttc ggcggctgtt ccgggtctaa ccccgctttc ttgaaggcgt ctttgttgta
    24301 atagagaacg gaagtcgagc tgttgaacgg ctgggagagt aagtggccgg ttttgctgtc
    24361 ggagtagtaa ccggaaaccg tcggcacaaa ctgcgactca tcgaactgaa tccctgcctc
    24421 tttaaacacg tcatacaccg gtttaatggc tttcgacgcc atcatggtgg cggtgccaac
    24481 ttcataaacc tgcaaaatag ccggagcgtt gccggtacga aatacggcaa tccccgcgct
    24541 taaattctgt tcgtagttgc ctttataggt cggtacaatt ttgtaatccg gattttcggc
    24601 gttaaaacgt tgggccagag aatccacctc tttacccagt tccccttcca tagaatgcca
    24661 gaacggaatg gtcgtcactg cctgtgcatt ccccattaac gccagtccga gcgccagtgc
    24721 tgaagctgta taatgtaacg gtttcatcgt ttatctctct tgttgtaccg aatgcgcgaa
    24781 ttcacgcgtt ttatgctcgc ggggtaacat gacatgctcg aattacagaa aaataacttt
    24841 tttgttacat ttgtaagata gtaaggtgtc agaaagatga caaggcggtg acggcgtggg
    24901 tgagggaaaa tgggagatgg ggcacggata agcggaaaaa tagaaggtct gaatcaaact
    24961 ctacagatta ctcatcgttt catgccggat gcggcgtaaa cgccttatcc ggcctacaag
    25021 atcgtgcaaa ttcaatatat tgcaactcac ccagtaggcc tgataagcgc agcgcatcag
    25081 gcaattttgc atttgtcacc tgtctcaaag gagtcttttg actccttatc aatcaacgtg
    25141 ttattacccg cctaaatacg cacttctcac tgcttcgttc gccagcagcg cgtcgccggt
    25201 atcggagagc accacatggc cgttttccag cacgtagccg cgatccgcca gctttagcgc
    25261 ctggttggcg ttctgctcga cgaggaagat agtcatcccc tgctcgcgca gctgctcgat
    25321 ggtgtcgaaa atttgctgga tgataatcgg cgcaagaccg agcgatggct catcaagtaa
    25381 caacaaacgc ggattgctca tcagcgcgcg accaatcgcc agcatctgct gttcaccgcc
    25441 ggacatggtg cccgcccgct gaacacggcg ctcatgcaga cgtggaaaca gttcatacac
    25501 ccactttatg cgctcctgaa actggtcacg ttcggcaaag aagccgccca tcgccaggtt
    25561 ctcttccacc gtcatccgcg agaagacgcg acgcccttcc gggacaatcg ctaccgcttc
    25621 gcgcatgatt ttcgccgtct gccagtcggt aatgtcttta tcatcaaaca caattcgccc
    25681 gctggtggcg cgcggatcgc cgcataacgt gccgagcaag gtggttttcc ccgccccgtt
    25741 cgcgccaatg agagtgacaa tctcgccctg attgatatgc aggctcacct catgcagcgc
    25801 ctggattttg ccgtagtggg cgctgacttt gtcaaaggac aacatgactt tttccatctt
    25861 atgcctcacc taaataggca cggatcacgt ccgggttatt acggatttgc tccggcgtac
    25921 cgtttgccag tggcgtcccc tgattgacca cataaattcg gtctgaaatc cccatcacca
    25981 gcttcatatc gtgttcaatc aacaaaatag tggtgttgtg atgattgcgc agttcggcaa
    26041 tcagctcatc cagctctttg gtttcttttg ggttaagacc tgccgccggt tcgtcgagca
    26101 ttaaaatctc cggctgcgtc accatgcagc gggcaatctc cagacggcgt tggtcaccat
    26161 aggccaggtt actcgcctga cggttggcgt gttccagcaa accaatgcgc tcaagccagg
    26221 tggcggcgcg gtcgagcgct tcgctctggg cgcgacggaa agaaggcgtt ttcaacaggc
    26281 cagagaacag cccggttttc agttgctgat gctgcgccac cagcaggttt tcaattaccg
    26341 tcatttcacg gaacagacgc acatgctgga aggtgcgcac cacgcccatg cgagcaattt
    26401 gctgccccgg taaaccttca agatgctgat cgcgcagtaa aatggtgccg ccggtgggtt
    26461 tgtagaaacc ggtcaggcag ttaaaaaccg tggtttttcc ggcaccgtta gggccgatta
    26521 acgagacgat ttcctgcggg tacagttcaa gattgacgtt gttcaccgcc agcaggccgc
    26581 cgaagcgcat catcagaccg ttaacagata ataatggctg actcatgcct gctctccttt
    26641 cgctgcgccg tttttcagct tcagttgcgg gcgcgtcatg ggcagcaaac cctgcggacg
    26701 ccagatcatc atcagcacca tcaaaccacc gagcattaac atgctgtatt cgttgaaatc
    26761 acgcatcaat tcgcgcgaca ccaccagcag aacagccgcc agaatcactg caaattgcga
    26821 gcccataccg ccgagcacca ctatcgccag cacaaaggcc gattcagcaa aggtgaagga
    26881 ttccgggctg acaaagccct gacgcgccgc aaacagcgtt ccggcaaaac cggcaaatgc
    26941 ggcgctgatg gtaaaagcag tcagcttgat acggcgcggg cttaagccca gcgaacgaca
    27001 ggcgatctcg tcttcacgca acgcttccca cgcgcggccc agcggcatcc gcagcaggcg
    27061 gttaatgaca aacaggctta acaccaccag cagcaacgcc accaggtaga ggaagataac
    27121 gcgatcggac ggatcgtatt tcaggccaaa gaaattactg aacgtgtccc agccgccttc
    27181 acgagcggta cggctgaact cgagtccgaa gagtgtcggt ttcgggatct gactgattcc
    27241 gttcgggccg ccggtgattt cagtgttatt gagcagcaat atgcgcacaa tttcgccgaa
    27301 accaagggta acgatcgcca gatagtcacc gcgcagacgc agcaccggaa aaccgagcag
    27361 gaaacccgcc gccgctgcca tcagtccggc aatcggcaaa caggtccaga accccagacc
    27421 gtaatagtga ttgagcagcg caaaagtgta agcgccgatg gcgtaaaaac cgccgtaccc
    27481 cagcaccagc agaccagaaa gaccaacaac cacattcagc ccgagaccga ggataatgta
    27541 gatcatggtc agagtggcga tatccaccgt cccacgtgac accataaacg gccacgccac
    27601 cacaagcacc agcagcgcca cgaggaacag cttttgcttc accgtggacc catcaatcgc
    27661 gggcagaata aacttcggcc cggaaacgct tttcaaccct ttctggaaag ccggtcgcaa
    27721 aagctggaag aaaaagacca ccgccgtgcc gataaacatc cactgccaac ggatgtctga
    27781 agccgtgtcg accaccagtt tggtgccatc cagctccagt tgtacgccca taaagacgcc
    27841 cgccaggaca aagaacatcg cggcagagag cagtgccatt gcaatatgca tcggtttcat
    27901 actttctcta cctccgggcg acccagaata ccggtcggca tcaccagcag caccagaatc
    27961 agcagggcga atgagaccac atctttatat tccgtactca gataggcaga agagagcgcc
    28021 tccgcaatcc ccaggatcag cccgccaatc atcgcccctg gaatgctgcc aatcccaccg
    28081 agcaccgcag cggtaaaggc tttcatcccg gccataaagc cgatataggg gttaatgacg
    28141 cagtagaact gaccgagcag tacgccagcc actgccgcca tcgccgcgcc aataacaaag
    28201 gtcaaagcaa tcacccggtc ggtgttgatg ccaagcaggc tcgccatttt cagatcttcc
    28261 gcgcaggcac gacaggcgcg ccccatacgg gaatagcgaa tgaaaatcgt cagcgccagc
    28321 atggcgagga aggtgacaat ccagatcacc gcctgcatgg tggtaataga ggcagagaag
    28381 ttttcgctat ggcccaccac ccactgaccg ttaaacaggc tcggcagcgc cacgtcgcgc
    28441 gaaccttcgg tcaggctgac gtagttttgc aggaagatgg acataccgat tgcggaaata
    28501 agcgcaatca ggcgcttaga gttacgcacc gggcggtaag ctacccgttc gatactccag
    28561 ccgtaggcgc tggcgatgac gattgcgccg acgaatcccg ccgccaccag cagccagccg
    28621 gtatcaatgc ccatcatcat cagcgcggcg atgatcataa atgagacgta gctgccaatc
    28681 atataaacct cgccgtgggc gaagttgatc atgccgataa tgccgtaaac catggtgtag
    28741 ccgatggcta tcagcgcgta ggtactgccc agcgtgacgc cgttaaacat ctgctgcaag
    28801 aaatacaaaa actgctcaga cataaggtaa cctttctaaa cccgcccgca ttttacgggc
    28861 ggtgggatga tcacttggct gccgtggatg aaccgtcggc gtgccactgg aagacaccaa
    28921 aatcaaatcc cttaagatcg cctttttcat cccagttcag cggcccaatc acggtgtttg
    28981 caccgttagc ttttaaatct ttcaccagcg ccagcggctc atcgctgccg gtacgctcaa
    29041 gggcagttgc cagagattgc actgccgcgt aagtgatcca gacataaggc ccggacggat
    29101 ctttcttgtc tgctttcagc gcatcaacga tgccctggtt tgccggatcc tggtcatagc
    29161 gttttggcat agtgaccaac atgccttcgg cggcatcacc ggcaatgttc gacagcgatg
    29221 cgttaccgac accttccggc cccataaact gggttttcag gccaacggaa cgggcttggc
    29281 gcagcatctg ccccatttcc gggtagtaac cgccgtagta aacgaagtcg atattttctt
    29341 ttttcaggcg ggcgatcagc gcggagaaat ctttctcccc ggcggtaata ccgtcgaaga
    29401 agacgacgtt ggcgttagcc gctttcagcc cgtcctgcac cgaacgcgcc agcccttcgc
    29461 catactgttg tttgtcgtga atgatggcga tgcgctgggg cttcaccgtc tcaagaatgt
    29521 attttgccgc cgtaggcccc tgggaagagt ccagcccggc agtacgcata atgtgttgat
    29581 aaccgcgttg ggtcagctcc gggttggtcg ctcccggcga gatcatcaga ataccttcgt
    29641 cttcgtagat atccgacgct ggctgggtag aggaagaaca cagatgacca ataacgtatt
    29701 taatgccgtc gttaacgatt ttgttggcga ccgcaacggc ttgtttcggg tcgcaggcgt
    29761 cgtcatattc cacgccaacc agcttgtcgc ccttaattcc ccctttgaca ttaatgtctt
    29821 taatcgcctg acgcgcgccg ttaaattcca tatcgcccca ctgggcaatc gggccggaca
    29881 tcgcgccgac aacggcgact ttaatatcgt cagccatagc ggtgtgtgaa attgccagtg
    29941 caatcatccc tgcgatgata gttttcgcat tccgtttcat agtcaaaaat ccccattcgt
    30001 gatgttgtgt tgctttgttt ttatgtgtta acaaatcaga ctgttctttt tttatactgc
    30061 actgtttttg cctgtctgat taaggggtta gcgcagtatt ttgtgatgat agcgattaaa
    30121 atccctattt ttcagtcgat taagaacaga taatattctg aatttattga tagataaaca
    30181 gaaaaaagtg cctttgtcag cataaaataa cggcacaaag ggcggaataa ttcactatca
    30241 ttcaggggat tatgctggac atttttcatt ctctaatgtt ttaactttgt aattattgct
    30301 gttaaaaaat taatcacctg ccaaaagaaa taaaaaagag aaagcctccg attaaattat
    30361 ttcgctacac tggttccact tttgtgattt acacgggtta cccatgaagc tgaccatcat
    30421 tcgattagaa aactttagcg accaggaccg gattgacctg caaaagatct ggccggagta
    30481 ttccccttcc tctttacagg ttgacgataa ccaccgtatc tacgccgcgc gttttaacga
    30541 gcgtctgctt gcggccgtgc gggtaacctt aagcggcacc gagggagcac tggattctct
    30601 acgcgtgcgg gaagtcaccc gccgtcgcgg tgtggggcaa tatctgctgg aagaggtttt
    30661 gcgtaacaat cctggcgttt catgctggtg gatggcggat gcaggcgtgg aagatcgcgg
    30721 tgtgatgacg gcgtttatgc aggcactggg atttacggca cagcagggcg gctgggagaa
    30781 gcgttaatcg tcaagttgat ttcaaaagtg atgaccaacg agaaattgcc tgatgcgcta
    30841 cgcttatcag gcctataatg tgtgctgcaa tttactgatt tctttggttc ttgtaggccg
    30901 gataaggcgt ttacgccgca tccggcatga agcaacgtat tcgatattag caatttggcg
    30961 gcaacccaaa gttgccgatt aacaattact tcgcatcggt cgccgtgccg ttggcgtgcc
    31021 agtcaaatac gccgaactca aagcccttta gatcgccttt ctcatcccag gtcagtggcc
    31081 ccatcacggt atccacggag ttcgctttta ggtatttggc gatttcagcc ggatcgtcag
    31141 actgattcag gcccgcctgc aaagattgca gcgcggcgta ggtggtccag acgaatgcgc
    31201 cgcttgggtc ctgttttttc gctttgatcg catcaacaat gggtttgttc gccggaacct
    31261 gatcgtagtt cttcggcttg gtcaccagca gcccttccgc tgattcgccc gcaatgttag
    31321 acagcgaaac gttagccaca ccttccggcc ccataaactg agttttcagc cctgccgcgc
    31381 gtgcctgacg caggatttgc cccatttccg ggtgataacc gccgtagtaa acgaagtcga
    31441 tattctcttt tttcagacgc gccaccagcg ttgagaaatc tttttccccg gcggtgatac
    31501 catcaaagaa caccacgtta gcattgcctt tcttcaggcc gtcctgcacc gctcgtgcca
    31561 gaccttcgcc gtattgctgt ttgtcgtgaa cgatagcaat acgctgcggt ttcactttct
    31621 caagaatata tttcgccgcc gtcggcccct ggtcggagtc caggccggtg gtgcgcagga
    31681 tcagctgata gccacgggcg gtcagctccg gcgcggttgc cgctggggtg atcattaaaa
    31741 tgccttcgtc ttcgtagatg tcagacgcag gctgcgttga tgaagaacag aggtgaccaa
    31801 tcacatattt aatgccgtcg ttaacgactt tgttcgccac cgcaaccgcc tgtttcgggt
    31861 cacaggcatc gtcatatttt acgatttgca gtttgttgcc tttaatgccg cctttagcgt
    31921 tgatatccgc aaccgcctgc tctgcgccgg taaactcctg gtcaccgtac tgcgcaaccg
    31981 gaccggacat tgcacccacg accgcgactt taatatcttc tgccagagcc atattgctga
    32041 atgccagcgc gatacatcct gccagtaacg ctttaccctt tatgttcatc ctgagaatcc
    32101 ccattcttct ggttattacg tgtgttgtga tgttgttgtt cagcacttta tttcgtttta
    32161 tgcatgacta cccgtgcttt agcagcatac tctgctaaaa catacccgat ttttatgata
    32221 ttggaatagc tattttgaca gtttattaac aatctgcgtg gggattggcg ttttgccgga
    32281 gggggaattg attattgtag aggcccaaaa aacaaaaacc ccggactctc atccagggtt
    32341 ctctgcttaa tagcggaaat tacgcttcaa tagcggcgcg aagttttttc atcgcgttct
    32401 tttccagttg gcgcacacgt tcagcggaaa caccgtaacg gtcagccagt tcctgcaacg
    32461 tggacttgtt gtcttcgtcc agccagcgcg cacggatgat gtcctggctg cgttcgtcca
    32521 gaccatgcat cgcgtcggtc agacggtttg ccgcctgctc ttcccagtta tcatcttcga
    32581 tgccgtcggc aaagttagat gatttatcct gcagatagag caccggtgcc atcggctggc
    32641 tgtcggaatc gtcgtcggaa gacaggtcaa aggtcatgtc ctgtgccgcc atacgtgatt
    32701 ccatctcacg tacgtctttg ctggttacgc ccagttcacg ggccaccatt tcgacttcat
    32761 cctggttaaa ccagcccaga cgctgcttgg ttttacgcag gttgaagaac agtttgcgct
    32821 gcgctttggt ggtcgcaact ttgacgatac gccagttacg cagaacgtat tcgtggatct
    32881 ctgctttgat ccagtggacg gcgaaggaga ccaggcgcac acccacttcc gggttaaaac
    32941 ggcgcactgc tttcatcagg ccgatgttac cttcctgaat caaatccgcc tgtggcaggc
    33001 catagcccgc ataattacga gcaatatgaa caacaaaccg caggtgagac aggatcagcg
    33061 ttttagctgc ttccagatcg ccatggtaat gcagcttttc agccagcgcc cgctcctcgt
    33121 cagccgacaa catcggccac gcgttagctg cccggatgta ggaatccagg ttgccaactg
    33181 gggctaaagc taaactttgc attttgtcag tcattcaaat cctctcaatc gatatcttct
    33241 ggcgcttcag tggtagcaac aactgtgcca aagcttaaga gcaacgaggt tatcattcac
    33301 tgttttatca gaccgtgatt ttatccacaa gttcaatgca agcttgtgaa taaattacgc
    33361 acaaaatgtg acatagagat gaaataccgg gaagagacaa cggggtctct ttccctgcta
    33421 cggaacccat tgcagggaaa gagtataaca cgcttttatt attcaggcgt aaagtggcgt
    33481 aaatgttgta ccgtggcaag ccacgctgcc acccagccaa tcatcgagca taccagcagc
    33541 aatagcaggc attcatcgaa cgataaacca ttgatatcaa acttcgttcc gaaaacctgt
    33601 gccacttccg ccaccgctga cgacaaacgc agtaccagaa tttctgacaa aattaatgac
    33661 aacaatgcgc cagaaaatcc cagcagcgca ccgccgtaca ggaacgggcg aaggatgaaa
    33721 ccatccgtcg caccaatcag tttctgcacg ttgatggagt cacggcgagc aaagatgctc
    33781 agacgtacgc tgttgccgat gacgaggaac actgccgcca ccatcaacac gccgatcatc
    33841 gccgaaacgc gcccaaccag cccggtcaac gccgccagac gggcaaacca gctgtcatcc
    33901 atccgcactt cgtcaatacc gttaatctgc gtgatacgat cacgcagcgt attgagtgat
    33961 tccgtcccct gaaaatcgag tttcgggatc accaccgcca ctgccggaag cgggttttct
    34021 tccagcatat ccagcgcacc accaaaacca gaccagttac ggaactcgcc cagtgcgtct
    34081 tcacgagaaa gatagttcac tttctccacg ccttgctcgg cctgcaactg tgccaccacg
    34141 cccgcagcgg cgtcatcgtc cagcgttttt tgcaagtaaa cagtgatttg cggtgacgga
    34201 taatactgcg tcgccgcctg attaacgttt ttgtacacca tataacagac gctgggcagc
    34261 gtcagggaga tggcgataac catcaccgtt aaaaacgtgg cgaacggttt gcttttcaga
    34321 tcctgcaatg cgccgtggaa ggcatagcgc acctgttcgt tgaaaacgtt ggttttgcga
    34381 tttaccggtt ttggcgagga tttcgcgcgt ttcggtgcgt tacgaccgcc gtcgcctgag
    34441 ccgccgaccg atttacggaa gcgatccaga cgcccaccaa actgccgaat atgattgatt
    34501 gcatcgcgct tattcatggc ccacgcctcc atgcaagtga ccatcgctca gggtgagcat
    34561 gcgataggaa cgccgtgaaa tcagattaat gtcgtgcgtt gccatcaata cggttacccc
    34621 aacgcggtta aactcttcaa acagacgtag aatcccttcc gacaacgcgt cgtccaggtt
    34681 accggtcggt tcgtccgcca gcagtaccgc gggcttattc accaccgcgc gggcaatgcc
    34741 aacacgctgt tgttcaccgc cggaaagctg aatagggaag ttcttcgctt tgtccagtag
    34801 cccgacttta tccagtgccg ccgacacccg gcgacgaata tcgtcaccgc tggcaccagc
    34861 gataatcagc gggatcgcca cgttatcgta gacagtacgg tccatcagta gatggtgatc
    34921 ctggaaaatc atgccaatct ggcggcgcag aaacggaact tcacggtttt tcagacgcgt
    34981 gatgtcatgg ccgctaaacc agattttccc ggcgctgggc cgctcaatcc cacagatcag
    35041 cttcaggagg gtacttttcc ctgcgccgga atgaccggtc agaaacgcca tctcacccgg
    35101 ctgcatatgg aacgtaacgc cctgcagcgc ctgtctccca ccgagataag ccttgctgac
    35161 atgttcaaag cgaatcattg ttaatcctct cgggcaaaaa gtgcctctat aaagtcgtcc
    35221 gccttaaacg gacgcaaatc ctcaatacgt tcgccgacac caatgtagcg gatagggata
    35281 ccaaactggt cagccaccga gaaaattacc ccgcctttcg ccgtgccgtc cagtttcgtt
    35341 agcgtgatgc cggttaagcc aacagcttcg tggaacagtt tggcctggct taccgcgttc
    35401 tgcccggtgc tggcatcaat agtcagcata acttcatgcg gcgcttcaac gtcgagtttc
    35461 ttcatcacgc ggacgatttt cttcaactct tccatcagat gcgatttgtt ctgcagacgt
    35521 ccggcggtat cggcaatcag gacgtcgata ttacgcgctt tagctgcctg aatggcgtcg
    35581 aagataacag aggcggaatc cgcaccggta tgctgggcaa tcaccggaat attgttgcgc
    35641 tgaccccaga cctgaagctg ttcaaccgca gctgcacgga aagtatcgcc cgccgccagc
    35701 atcaccgatt taccctgctg ctcaaactga cgcgccagct taccaatcgt cgtggtttta
    35761 cccacaccgt tgacgcccac catcaggatc acgaacggcg ttttatcttc aacattcagc
    35821 ggctcatcga ctttcgccag aatctcgccc atctcttctt tcagcaggcc atagagcgcc
    35881 tcggcatcac gaagctgctt gcgggatgcg ccttccgtca gattggtgat aattttacgt
    35941 gtggtttcca cgcccacatc ggcgatcaac agctgttctt ccagctcttc aaacagatca
    36001 tcgtcgattt ttttaccgcg gaacaggctg ataaatccgg aaccgagatt ttctttggtt
    36061 tttaacaggc tgcgtttcag gcgcgcgaag aagccttctt tggtcggttt ttcttgctcc
    36121 tgggcaattt ctgcaaccgg ctgctcttct tctgccggag gaactaccat caccgcctct
    36181 tctgccgctt cggcagccag cgcctgtgct tccagctctt cgtcggtgat ttcttcttta
    36241 gccgcttctt cttctgccgc ttcgacaatc tctacggttt ccgcttcagc ctgccactct
    36301 tctgacgaaa cctcttcggc gttgacgtct tccggcaacg gcagctcttc acgttcgata
    36361 gccactggct ccggcgtttc ttctacgacc ggttccggct gtgcaacaac ttccgcttca
    36421 ggctgcgctt tttcactttc aacaacctgt tcagtgactt ccacaacgtc ggcagcaaaa
    36481 gtttccgcct cggcttcagt atgcgcctgc ggctgctctt caacggcttg ttcagaagcc
    36541 ttcgcaggct cttgcgcctg aacgatttct tctacaaccg gttgttcatt ctgaacttct
    36601 gtctcttttt ccggggtctg ctctttttga ccaaagccca gccaggaaaa aaagccacgt
    36661 cttttttctt tcgccatttg cgactacact cctcgctgtt gcttcatggc acagcgttaa
    36721 cgctatgtac atagcagcta aaaaaatgat gaaatagtct atcacttaac ttaattcaca
    36781 tcaccgcctg caagtatgtg ttatctggcg gattgagcaa tttatcatga aaaaaccgaa
    36841 tcattccggc agcggccaaa tccgcattat tggcgggcag tggcgaggcc gtaaactccc
    36901 ggttcctgat agcccaggtc tgcgccccac caccgaccgc gtacgcgaaa cgttgtttaa
    36961 ctggctggct ccggtcattg ttgacgccca atgtctggat tgcttcgccg ggagcggcgc
    37021 gctggggctg gaagcgttat cgcgctacgc tgcgggggca acgttgattg agatggatcg
    37081 cgtggtttct cagcagttaa ttaagaatct ggcgacacta aaagcaggca atgcacgcgt
    37141 ggtgaacagc aacgcaatgt cattcctggc gcaaaaaggc acaccgcata atatcgtgtt
    37201 tgtcgatcca ccgttccgcc gtggcttgtt agaagagacg ataaatttac tggaagataa
    37261 cggctggctg gctgacgaag ccctgattta tgtcgaaagc gaagtcgaaa acggtctgcc
    37321 cactgttccg gcaaactggt cattacatcg ggaaaaagtg gcgggtcagg tggcttatcg
    37381 gctgtatcaa cacgaagcac aaggagaaag tgatgctgat taatattggt cgtttgttaa
    37441 tgctctgcgt ttggggattt ttaatcctca atctggtgca tcccttcccg cgcccgctaa
    37501 atattttcgt taacgtggcg ctgattttta ccgtgctgat gcatggtatg cagctggcgc
    37561 tattgaaatc cactttaccg aaagatggcc cgcagatgac caccgccgaa aaggtgcgga
    37621 ttttcctttt cggcgtgttt gaactgctgg tctggcagaa gaaatttaaa gttaaaaaat
    37681 aaccttattg ttcgccaaca aagctaacaa agcgcgttcc tttatagctc agcgtacctt
    37741 tatcgcccac tgtcagggcg tggtactgct gggcgtcgag gcgaaacgtc tgctccattc
    37801 ctccgctttg cggtttgaag cttgcctcat agcgcatact ggtgcctgcc ggagtcactt
    37861 cctgctggcg cgaacggcga tcgttaatcg gtttttcccg cttgttgctc accaccacca
    37921 gcttttgctg gagcggagcc atatcattat cagctttttc ccgtcgctgt tgcataaaac
    37981 gaaacgatgc ggcgacgaca attaagccaa tgataacaat aaagaaaaga ggtggtttac
    38041 tcatctttat ccctcatcgg aaaatgcgga attaagcata ccctgccagt tatggtgttg
    38101 tcatccgtcc accctcgcca ctaaactgga agcaagaccg taggcattct gcttacgaaa
    38161 aaataacgaa ttcaaggaac taagatgctt tggtcgttta tcgctgtctg tctttccgca
    38221 tggctatctg tggatgcatc gtatcgtggg ccaacctggc aacgctgggt gtttaaaccg
    38281 ttaacccttc ttctcctgct gttactggcc tggcaagcac cgatgttcga cgccattagc
    38341 tatctggtgc tggcgggact gtgcgcctca ctgctgggcg atgcgctaac cctgttgcca
    38401 cgtcaacgtc tgatgtacgc catcggcgcg tttttcctct cgcacctgct gtacaccatc
    38461 tatttcgcca gtcagatgac gctctctttc ttctggcctc tgccactggt gttgctggtt
    38521 ctgggtgcgc tgctactggc gattatctgg acgcggctgg aagagtaccg ttggcctatc
    38581 tgcacgttta tcggcatgac gctggtgatg gtgtggctgg caggtgaact gtggttcttc
    38641 cgtccgaccg ctccggcgct ctctgcgttt gttggcgctt cgttgctgtt tatcagtaac
    38701 tttgtctggc tggggagcca ctatcgccga cgcttccgtg cggataacgc gattgctgcg
    38761 gcctgctact ttgccggtca cttcctcatc gtccgctcac tgtatctctg ataaaacttg
    38821 actctggagt cgactccaga gtgtatcctt cggttaatga gaaaaaactt aaccggagga
    38881 tgccatgtcg acacctgaca atcacggcaa gaaagcccct caatttgctg cgttcaaacc
    38941 gctaaccacg gtacagaacg ccaacgactg ttgctgcgac ggcgcatgct ccagctcgcc
    39001 aacgctctct gaaaacgtct ccggcacccg ctatagctgg aaagtcagcg gcatggactg
    39061 cgccgcctgt gcgcgcaagg tagaaaatgc cgtgcgccag cttgcaggcg tgaatcaggt
    39121 gcaggtgttg ttcgccaccg aaaaactggt ggttgatgcc gacaatgaca tccgtgccca
    39181 ggttgaatct gcggtgcaaa aagcgggcta ttccctgcgc gatgagcagg ccgccgacga
    39241 gccacaagca tcgcgcctga aagagaatct gccgctgatt acgcttatcg tgatgatggc
    39301 aatcagctgg ggtctggagc agttcaatca tccgttcggg caactggcgt ttatcgcgac
    39361 cacgctagtt gggctgtacc cgattgctcg tcaggcatta cggctgatca aatccggcag
    39421 ctacttcgcc attgaaacct taatgagcgt agccgctatt ggtgcactgt ttattggcgc
    39481 aacggctgaa gctgcgatgg tgttgctgct gtttttgatt ggtgaacgac tggaaggctg
    39541 ggccgccagc cgcgcgcgtc agggcgttag cgcgttaatg gcgctgaaac cagaaaccgc
    39601 cacgcgcctg cgtaacggtg agcgggaaga ggtggcgatt aacagcctgc ggcctggcga
    39661 tgtgattgaa gtcgccgcag gtgggcgttt gcctgccgac ggtaaactgc tctcaccgtt
    39721 tgccagtttt gatgaaagcg ccctaaccgg agaatccatt ccggtggagc gcgcgacggg
    39781 cgataaagtt cctgcaggag ccaccagcgt agaccgtctg gtaacgctgg aagtgctgtc
    39841 agaaccgggt gccagcgcca ttgaccggat tctgaaactg atcgaagaag ccgaagagcg
    39901 ccgcgccccc attgagcggt ttatcgaccg tttcagccgt atttatacgc cagcgattat
    39961 ggccgtcgcc ctgctggtaa cgctagtgcc gccgctgctg tttgccgcca gctggcagga
    40021 gtggatttat aaaggtctga cgctgctgct gattggttgc ccgtgtgcgt tagttatctc
    40081 aacgcctgcg gcgataacct ccgggctggc agcagcagcg cgtcgtgggg cgttaattaa
    40141 aggcggtgcg gcgctggaac aactaggtcg tgttacccaa gtggcgtttg ataaaaccgg
    40201 tacgctgacc gtcggtaaac cgcgcgttac cgcgattcat ccggcaacgg gtattagtga
    40261 atctgaactg ctgacactgg cggcggcggt cgagcaaggc gcgacgcatc cactggcgca
    40321 agccatcgta cgcgaagcac aggttgctga actcgccatt cccaccgccg aatcacagcg
    40381 ggcactggtc gggtctggca ttgaagcgca ggttaatggt gagcgcgtgt tgatatgcgc
    40441 tgccgggaaa catcccgctg atgcatttgc tggtttgatt aatgaactgg aaagcgccgg
    40501 gcaaacggtt gtgctggtag tacgtaatga tgacgtgctg ggtatcattg cattgcagga
    40561 taccctgcgc gccgacgcta caactgccat cagtgaactg aacgcgctgg gggttaaagg
    40621 agtgatcctc accggcgata atccacgcgc agcggcggca attgccgggg agctggggct
    40681 ggagtttaaa gcgggcctat tgccggaaga taaagtcaaa gcggtgacca agctgaatca
    40741 acatgcgccc ctggcgatgg tcggtgatgg tattaacgac gcgccagcga tgaaagctgc
    40801 cgccatcggg attgctatgg gtagcggcac agacgtggcg ctggaaaccg ccgacgcagc
    40861 attaacccat aaccacctgc gcggcctggt gcaaatgatt gaactggcac gcgccactca
    40921 cgccaatatc cgccagaaca tcaccattgc gctgggactg aaagggatct tcctcgtcac
    40981 cacgctttta gggatgaccg ggttgtggct ggcagtgctg gcagatacgg gtgcgacggt
    41041 gctggtgacg gcgaatgcgt taaggttgtt gcgcaggaga taaggcaaac cgatcgcaac
    41101 attgagcgcg atcggtcccc tcgcccctct ggggagaggg ttagggggtg aggaaaaggc
    41161 ggcatcgaag ccaatcagcc cctatcaacc gcctttacga atcaaataac gataaggcag
    41221 tccatccgtc tctttcgcaa ccagttcgtg ttccataaag gtacaaaacc caggaatatc
    41281 gcgggtagtg gccggatcgt cggcgataat cagcaacgtt tcgccaggct gcatattgcg
    41341 cacggttttg cgcaccatca tcaccggttc cgggcagcgc aggccaagcg cgtcgagtgt
    41401 gtggtcaggg ctggaaaaga gatcggtcat tttcttctca tcacttaaaa aaacggcgct
    41461 agtttacgcc ctgtgagtcc gtaagcaaac caggttaacg attgcgtgaa aattaaccat
    41521 tgcattgtca acgtaaagca gtatcatgcg gcggctcgaa aaaagggtaa gcacgttatt
    41581 atgttaaggt aacagacgtg tcgtacgtat tgggttccct caccccaatg gttaatcaaa
    41641 aaggtacaat atgaacgttt tctcgcaaac tcaacgctat aaggcgttgt tctggttatc
    41701 gttatttcat cttctggtga tcacctccag taactatctg gttcagcttc ccgtctccat
    41761 ttttggtttc cataccacct ggggcgcgtt tagctttccg tttatttttc ttgctaccga
    41821 cctgaccgtg cgtatttttg gcgcaccgct ggcccgacgc attatcttcg cggtaatgat
    41881 ccctgcgtta ttaatctcct acgtcatctc gtcgctattc tatatgggtt cctggcaggg
    41941 attcggcgca ctcgcccact tcaacctgtt tgtcgcccgt atcgccaccg ccagtttcat
    42001 ggcctacgcg ctggggcaaa tcctcgacgt gcacgttttt aaccgcctgc gtcagagtcg
    42061 ccgctggtgg ctggcaccga cagcgtccac actgttcggt aacgtcagcg acacgctggc
    42121 ctttttcttc attgccttct ggcgtagccc ggatgccttt atggctgaac actggatgga
    42181 aatcgcgctg gtcgattact gtttcaaagt gttaatcagt atcgttttct tcctgccaat
    42241 gtatggcgta ttactcaata tgctgttgaa aagactggca gataaatccg aaatcaacgc
    42301 tttgcaggcg agttaaaggt tcgttatcag agttgtgata agatggatga atgagccgtt
    42361 atggccgttt atcgaaagga agaagtcaat gcgcaatctg gttaaatatg tcggaattgg
    42421 cctgctggtt atggggcttg cggcctgtga tgataaagac actaacgcta cggcgcaggg
    42481 ttcggttgcg gaaagtaacg ctaccgggaa tcccgtcaac ctgcttgatg gcaagttaag
    42541 tttctcgctg ccagcggata tgaccgacca gagcggtaag ctgggaacgc aggccaataa
    42601 catgcatgtc tggtccgacg ccaccgggca gaaagcagtc atcgtcatca tgggcgatga
    42661 tccgaaagaa gatctggcgg tgttggcgaa gcgtctggaa gatcagcaac gtagccgcga
    42721 tccgcagctg caagtggtaa ccaataaagc cattgagctg aaaggtcaca aaatgcagca
    42781 gttagacagt attatctccg cgaaaggcca gacggcgtac tcttccgtta ttctgggtaa
    42841 cgtgggtaat caactgctga ccatgcaaat tacgctgcca gctgacgatc agcaaaaagc
    42901 gcaaaccacc gcagaaaaca tcattaatac gctggttatt cagtaagttt taagatgatg
    42961 aggcggcctc agggacgtat tccggaggcc gtttttttaa tcgccacgtc agtaataacg
    43021 cgattgcgac cagtcccgcc gccgccagat aaatcaccgg tacgcccgcc cagctcatca
    43081 ccagcccagc cagtggtcca gtcacgccaa gcgataaatc cataaatacg gtgtaagttg
    43141 ccagcgccgc cccctgattt tgctgcggaa ccgcttttac cgccactaca cccaatgccg
    43201 ggaacaccag cgaaaacccg gcccccgcca gtaagacgcc gattttcgcc atccacggca
    43261 tagtcgccac gccaaccagt agcaggccga ttatctcaac gctaaagcaa atcatcgcta
    43321 cgtttaagcc accgatacgg ttaatgccgt tagggaataa caaacgcgta ccgacaaacg
    43381 cacagctaaa cagcgtcagc gcgaaagccg caccgtccca acctttagcg tcataaaaca
    43441 gcgtgataaa ggtggcgatg acgccaaatc cggcggaagc cagtgccagc gccataccgt
    43501 acagccagac gcgcccaagc accgcgcgaa acggcagcgg tttgccttta ctggctttta
    43561 ccgtcggacg cgggatcgcc aacaaaatgg ccaccagcgc cacgcccata atgattaacg
    43621 ctaacgcctg caagccgccc cagtgataaa acacgacgcc taacggcgca cccatcgcca
    43681 tcgccccgta agtgacaatg ccgttccacg aaatcacccg cccgatatgc agcgagccaa
    43741 ccactccaac gccccatagg gtcgatcccg ttccggcaaa actttgccca atcccaagga
    43801 tgacgcgccc caggcaaagt aataacaggc tgatgacagg cagactggcg gttaatcctg
    43861 ccgtcagata ccccagaccg ctcaaaaagc agccgcataa accgaagacg acaatctttt
    43921 tgggtcccag caaatcggcg taacgtccgg catgagggcg gctcagcaag gtggcgaaat
    43981 attgcaggct gataaccaat cctgcccaga aggcgctaaa gcccatcaca tcatggacat
    44041 agcccggtaa tacagcgagc ggcaacccga tggtgaggta gctggcgaag ttaaacatga
    44101 caatggagac aatgcgcaaa ttcaggcgca atccgtttag cgcgggttcg gctacgggtt
    44161 cgggcatgag gatcaccaca tttttacaac agtgtttcat ttttaccatg tgctgacgtg
    44221 aaaatcagca gtaagaatca gaatattgct ggcgtgactc ccgcgctaca cttaacacaa
    44281 aaagtcacaa ggaagcccca atggaaaccc ctcaacccga taaaacgggc atgcacattc
    44341 tgctcaagtt ggcctcgctg gtagtgatcc tcgcgggcat tcacgcagcg gcagatatca
    44401 ttgtgcagct gttactggcg ctgttttttg ccatcgtcct caacccgctc gttacctggt
    44461 ttattcgtcg gggagtacaa cgccccgttg ccattacgat tgtagtggtg gtgatgctga
    44521 tcgcactaac cgcgctggtc ggcgtactgg cggcatcgtt taacgaattt atctctatgc
    44581 tgccgaagtt taataaggag ctgacgcgca aactttttaa attgcaggag atgttgcctt
    44641 ttcttaattt gcatatgtcg ccggagcgaa tgctgcagcg gatggactcg gaaaaagtgg
    44701 ttaccttcac cacagcgcta atgaccgggc tttccggggc aatggcgagc gtgcttttgc
    44761 tggtgatgac cgtagttttt atgctgtttg aagtgcgcca cgtcccttac aaaatgcgtt
    44821 ttgcgctgaa taacccacag attcacatcg caggattaca ccgcgcactt aaaggcgttt
    44881 cgcattatct ggcgttaaag acgctactca gtttatggac aggtgtcatc gtctggctgg
    44941 ggctggcgct aatgggcgta cagtttgcgc tgatgtgggc agtactggcg tttttgctca
    45001 actacgtgcc caatatcggc gcggtaattt ccgccgtacc gccaatgatt caggtgctgc
    45061 tgtttaatgg tgtttacgaa tgtattctgg tcggcgcatt gtttttagtg gtccatatgg
    45121 tcatcggcaa tattttagaa ccacggatga tgggccatcg cctggggatg tccaccatgg
    45181 tggtatttct ttcattgtta atctggggat ggctgctcgg cccggtaggg atgctacttt
    45241 cggtgccatt aaccagcgtg tgtaaaatct ggatggaaac gacgaaaggt ggcagcaaac
    45301 tggcgatttt actggggccg ggcagaccga aaagtcggtt accgggatga ggcgacaagt
    45361 gatacgatac gcactttcat tttccattaa acgttggccc tgatatgtat cggatagttc
    45421 tggggaaagt ttcgacctta agcgcagctc cactgccacc gggtttacgc gagcaagcac
    45481 cgcaaggtcc acgacgcgaa cgctggctgg cggggcgtac attgctttcg cacacgcttt
    45541 ccccgctacc ggagatcatc tatggcgaac aaggcaaacc tgcattcgcg ccggaaacgc
    45601 cgctatggtt caacttaagc catagcggtg acgatatcgc tctgctgttg agcgatgaag
    45661 gcgaagtcgg ctgcgatatc gaagtgattc gcccgcgcgt caactggcgc tggctggcga
    45721 acgccgtgtt cagcctcggg gaacacgctg aaatggacgc cgtgcatcct gatcagcaac
    45781 tggaaatgtt ctggcgcatc tggacgcgca aagaagccat cgttaaacag cgtggcggca
    45841 gcgcctggca aatcgtcagc gtagatagca cctatcactc ctcgctgtca gtcagccatt
    45901 gtcagctaga aaatttaagc cttgcgatct gcacccccac cccctttacg ctcaccgccg
    45961 acagtgtgca atggatcgat tcagttaact gatccgccca cccgactgcc catctattga
    46021 tccagaacag gtaatcagta tgacgaatac ttaaaatcgt catacttatt tccgccatct
    46081 attttaatcc attggggtta ccatgctctc cacactccgc cgcactctat ttgcgctgct
    46141 ggcttgtgcg tcttttatcg tccatgccgc tgcaccagat gaaatcacca ccgcctggcc
    46201 ggtgaatgtc gggccactaa acccgcacct ttacacgcct aaccagatgt tcgcccagag
    46261 catggtttat gaaccattgg tgaaatatca ggcagacggt tcggtgatcc cgtggctggc
    46321 aaaaagctgg actcattcag aagatggtaa aacctggacc ttcaccctgc gtgatgacgt
    46381 gaaattctcc aacggtgaac cgttcgatgc cgaggcggcg gcagaaaact tccgcgcagt
    46441 gctcgataac cgtcaacgtc acgcctggct ggagctggca aaccagattg ttgatgttaa
    46501 agcactcaat aaaacagagc tgcaaattac cctgaaaagc gcctactatc ctttcctgca
    46561 agaactggcc ctgccccgtc ctttccgttt tatcgctccc tcgcagttta aaaaccatga
    46621 aaccatgaac ggaattaaag cgccgattgg caccggaccg tggattttgc aggaatcgaa
    46681 actaaatcag tacgatgtct tcgtccgtaa cgaaaactac tggggcgaaa agccagcgat
    46741 taaaaagatc acctttaacg tcatcccgga cccgactacc cgcgcggtgg cgtttgaaac
    46801 tggcgatatc gacctgctgt acggaaacga agggttatta ccgctcgata ccttcgcccg
    46861 ctttagccag aatccggctt accacaccca actgtcacag ccgatcgaaa ccgtgatgct
    46921 ggcgctcaat accgccaaag cccccaccaa cgagctggca gtacgtgaag ctcttaatta
    46981 cgcggtaaac aaaaaatcgc tgattgataa cgcgttatat ggcacccagc aggtcgccga
    47041 caccctgttt gccccttctg tgccttacgc taatctcggc ctgaaaccgc gccagtacga
    47101 tccgcagaaa gcgaaagagt tgctggaaaa agccggttgg acgctgcctg cgggcaaaga
    47161 catccgcgag aaaaatggtc agccgctgcg cattgaactg tcgttcatcg gcaccgatgc
    47221 gttaagcaaa tcgatggcgg aaatcattca ggctgatatg cgccagattg gcgcagatgt
    47281 ctcgctgatt ggcgaagaag agagcagtat ctatgctcgt cagcgcgacg gtcgttttgg
    47341 catgattttc caccgcacct ggggcgcgcc atacgatcca cacgccttcc tgagttcaat
    47401 gcgcgtaccg tcgcacgctg acttccaggc acagcaagga ttagccgaca agccgctgat
    47461 tgataaagag atcggcgaag tgctggcgac ccatgacgaa acgcaacgtc aggcgctgta
    47521 tcgcgacatt ctgacccgtc tgcatgacga ggcggtttat ctgcctatca gctacatctc
    47581 gatgatggtg gtatcaaaac cggagctggg taatatcccc tacgcgccga tcgccaccga
    47641 aattccgttc gaacagatta aaccggtgaa accttaatgt tgcgttacgt attacgccgc
    47701 tttctgctgc tgatcccgat ggtgctcgcc gcctcggtga tcatttttct gatgctgcgc
    47761 ctcggtaccg gcgacccggc gctcgattat ctgcgtttat ctaacctgcc gccgacgccg
    47821 gagatgctgg cctctacccg caccatgctc gggctggatc agccgctgta cgtccagtac
    47881 ggtacctggt tgtggaaggc gctgcatctt gactttggta tctcattcgc cagccaacgc
    47941 ccggtactgg acgatatgct gaacttcctg cccgccacgc tggaacttgc aggtgcggcg
    48001 ctggtattaa ttctgctcac ttccgtaccg ctcggtatct gggcggcgcg ccaccgcgat
    48061 cgtttgccgg atttcgccgt gcgctttatc gcgtttcttg gcgtgtcgat gcccaacttc
    48121 tggctggcat ttttgctagt gatggcgttt tcggtgtatc tgcaatggct acccgcgatg
    48181 ggctacggca gctggcagca catcatttta cctgcggttt ccatcgcctt tatgtcgctg
    48241 gcgattaacg cgcgactgct gcgcgccagt atgctggacg tcgccggtca gcgtcacgtc
    48301 acctgggcgc ggctacgcgg cctgaatgac aaacagaccg aacgccgcca tattctgcgc
    48361 aatgcctcgc tgccgatgat caccgccgtg gggatgcaca ttggcgaatt gattggcggg
    48421 acgatgatta tcgaaaacat ctttgcctgg cctggcgtcg ggcgctatgc ggtgtcggcg
    48481 atttttaacc gtgactatcc ggtgattcag tgctttacgc tgatgatggt ggtggttttt
    48541 gtggtctgta atttgattgt cgatttgctc aacgccgcgc tggacccgcg cattcgtcgt
    48601 catgaaggag cgcacgcgtg aactttttcc tctcttcccg ctggtcggta cgcctggcgc
    48661 tgatcattat cgccctgctg gcgctgattg cgctcaccag ccagtggtgg cagccgtacg
    48721 atccacaggc aattgatttg ccgtcgtgcc tgctttcgcc ggatgcgcag cactggctgg
    48781 gcaccgatca cttaggtcgc gatattttct cgcggctgat ggcagcgacc cgcgtgtcgc
    48841 tcggttcggt aatggcctgc ctgctgctgg tgctgacatt agggctggtt attggcggca
    48901 gcgccgggtt gattggcggg cacgttgatc aggccaccat gcgcgtcgcc gatatgttta
    48961 tgaccttccc gacctcgatt ctgtcgttct ttatggttgg cgtgctcggc accgggctga
    49021 ccaacgtaat tatcgccatc gccctgtcgc actgggcgtg gtatgcacgc atggtgcgca
    49081 gcttggtgat ttcactacgc caacgcgagt ttgtgctggc gtcacggctt tccggtgcgg
    49141 gccatgtgcg ggtgtttgtc gatcatctgg caggcgcggt gatcccttcg ctgctggtgc
    49201 tggcaacgct ggatatcggc catatgatgc tgcacgtcgc ggggatgtct ttccttggcc
    49261 tcggtgtgac cgcgccgacc gccgaatggg gcgtgatgat taacgacgcg cgccagtata
    49321 tctggaccca gccgctgcaa atgttctggc cggggctggc gctgtttatc agcgtgatgg
    49381 cctttaacct ggtgggtgac gcactgcgcg atcatctgga tcctcatctg gtgacggagc
    49441 acgcacacta atgccgcaac agattgaact acgtaatatc acgctacagg ccgcgcagcc
    49501 gctggtacac ggtgtatcgt taaccctgca acgcgggcgc gtgctggcgt tagtcggcgg
    49561 tagcggcagc gggaaatcat taacctgcgc cgcgacgctg ggcattttgc ccgctggcgt
    49621 tcgccagacg gcgggggaaa ttttagccga tggcaaaccg gtttcgcctt acgccctgcg
    49681 cggcatcaaa attgccacca tcatgcagaa cccgcgcagc gcctttaatc cactgcacac
    49741 catgcacacc cacgcgcggg aaacctgcct ggcgttaggg aaacccgccg atgacgctac
    49801 gcttaccgct gccatagaag cggtggggct ggaaaacgcc gcgcgcgtgc tgaagctgta
    49861 cccgttcgag atgagcggcg gcatgttgca gcgcatgatg attgcgatgg cggtgctgtg
    49921 tgaatcaccg tttatcatcg ccgatgaacc gaccaccgac ctcgacgtgg tagcacaggc
    49981 gcgcatcctc gatctgctgg aaagcattat gcaaaaacaa gcgccgggaa tgctgctggt
    50041 cacccatgat atgggcgttg tggcgcgtct ggcggatgac gtggcggtga tgtcacaagg
    50101 taaaattgtc gaacagggcg atgtagaaac gctgtttaac gcccccaaac atacggtgac
    50161 gcgcagcctg gtttccgctc atctcgccct ctacggtatg gagctggcat catgacctta
    50221 cttaacgtct gcggcctttc ccatcactat gcccacggtg gatttagcgg aaaacatcaa
    50281 catcaggcgg tgctgaataa cgtttccctg accctgaaaa gcggcgaaac cgtcgccctg
    50341 ctgggacgca gcggctgcgg aaaaagcacc ctcgcgcggc tgctggtagg cttagagtct
    50401 ccctcacaag ggaatattag ctggcacggc gaatcgctgg cgaaacttaa tcgcgcccaa
    50461 cgtaaagcct tccgccgtga tattcagatg gtgtttcagg actccatcag tgccgtgaat
    50521 ccgcgcaaaa ccgtgcgcga gatcctgcgt gaaccgatgc gccacctgct atcactgaaa
    50581 aaatccgaac aactggcgcg cgccagcgaa atgctgaagg cggtcgatct cgatgacagc
    50641 gttctcgaca aacgcccacc gcagttaagc ggcggccagc tccagctcgt ctgcctggca
    50701 cgcgcgctgg cggtcgaacc gaaattgctg attctggatg aagccgtttc taaccttgat
    50761 ctcgtgttac aggcgggcgt cattcgcctg ctgaaaaagc tacaacaaca gtttggcact
    50821 gcctgcctgt tcatcactca tgacttacgc ctggtggaac gcttttgcca gcgagtgatg
    50881 gtgatggaca acggacaaat cgccgaaacc caggcggtgg gagataaatt aaccttttcc
    50941 tctgacgccg gacgtgtgct acaaaacgcg gtattacccg cattccccgt gcgccgtcgc
    51001 acctcagaaa aggtttaacg ccaatgcaac gagtcaccat cacgcttgat gacgatttac
    51061 tggagacgct ggacagcctg agccagcgtc gtggttataa caaccgttcc gaagctatcc
    51121 gcgatattct gcgtagtgcc ctggcgcagg aagctaccca gcagcacggc acacaaggtt
    51181 tcgcggtgct gtcgtatgtg tatgaacacg aaaaacgcga cttagccagc cgtattgttt
    51241 ccacccagca tcatcaccac gacctctccg tcgccacgct acatgtgcac atcaaccacg
    51301 acgactgtct ggaaatcgcc gtgttgaaag gtgacatggg tgacgtgcag cattttgccg
    51361 atgacgttat cgcccagcgc ggcgtgcggc acgggcattt gcagtgcctg ccgaaggaag
    51421 attgagttat ttctaaaaca aggaaagaaa agatattaac tgaacttaat ttataaaatt
    51481 aagttcagtt gcatagatag atcctgtaaa ggcagatatt ctgcgtaatg tacgtcgcta
    51541 aaatttataa atgattcaaa cgctcattca gtgtttcaat aatataggta ttaacagaga
    51601 cttgttgctg cgcagccgca ttattaaggc gttcactcag cgattctgga tagcgtaacg
    51661 taaaggtctt aatcttttct gtacgcgcat acggctcaat acccgcagct ttacaatcct
    51721 caagatactc tcgcagggaa agctctcctt ctttttgcag cccctgaata ctgtcagaga
    51781 caaaatcaca ataacctgac aagcccagga atttgccacg aaatgcattc agttctggca
    51841 cgtaggtaat aacagctggt tgcccggcaa tctccataga gtttggcgtc ttcagtttga
    51901 tcatggtttt acccctatac ttagtaacca gtcacacacg ctttctacag cgcctttatc
    51961 ggtatctggc gacggatgag gccgatggaa acacgcaacg ctcatattta gtatgaactt
    52021 gcaacgcgaa cctcttcctt ccttaatttc tccgcctaat gctttaacca gtgactctat
    52081 atcagaccac ttgatccctt gaggaacagg agttttaaat atttgatcca atgtattttt
    52141 ttgtttcttc cgtaaagcct gtaccttggt tctcatggca tgtccttatg acttcacttt
    52201 atgacatcac agtatgaagt caagtttccc ttccttacgc cattgtccca atcgtcttcc
    52261 tgaatcgcag cagtgcgatg gtgaaaaacg cgccgccaat tgccatcagc gtcaggaact
    52321 gcggccagac gatttcgaat ccggcacccc ggtagaggat ggcctgcgcg aggctaacaa
    52381 agtgtgtcgt cggcatggtc agcataatgt cctgcaccat ctgcggcata ctttcgcgcg
    52441 gcgtggatcc accggaaagc atttgcagcg gcagcagcac cagaatcacc agcagcccca
    52501 gttgcggcat tgaacgcgct atcgtcccca taaaaatgcc gattgacgtg gtggcaaaca
    52561 gactgagcgc cacgcccagc ataaacagcg ggatcgagcc ttcaatcggt acgcccagta
    52621 cacctttcac catcagcacc agcgataatc ccgataccac cagcaccacc agccccatcg
    52681 accagatctt cgccatcatg atctcaaacg gcgttatcgg catcaccagt aaatgttcca
    52741 ccgtgccgtg ttcacgctcg cggatcagcg ccgatccggt caatacaatc gccagcatgg
    52801 taatgttgtt gatgatcgcc atcaccccgc caaaccacgc gggatcaagg ttcgggttaa
    52861 agcgcatccg ggtttccagc gataccaacg gttcgctgtt atcacggtag cgcgcgacaa
    52921 agctgttcac ttcaccgttg ataatattct ggatataccc attgccggta aatgcctggc
    52981 tcatgcgcgt ggcatcgacg ttcacctgaa tatccggctg gcgtccggcg aggacatcac
    53041 gctgaaaatt aggcggaata tttatcgcga aggtatagcg tccggcgtcc agtccggcat
    53101 ccatctcatc ggcggtgatc atctccggtg gcaaaaacca cggacgatag aagctgttaa
    53161 cgatccggtt cgataactgc gattgatcca tatcggcaat ggcgatcggc gcgaggttca
    53221 acgatcctgg cgtaacggtc gctgacgaat acaccgacac cgtaaacgag aagacaatca
    53281 gcgtcagcat cgctttatca ccgagcagac tgcgcaactc tttgataccc agattaaaaa
    53341 tattgcgtaa atggcgcatc atccctcctg ttttttcagc agcaggatgc ttaagcccat
    53401 caccagcggg atggctatca gcaacgggat aaaaagttgc cacaaatcag tcagatccag
    53461 cgctttcgag aacgttccgc gggcgatagt cagaaaatga ctggtcgggt aaacctcgcc
    53521 gatccaacgt ccaggccctt ccagcgaagc taccggatcg atcatcccgg aaaactgtgt
    53581 cgccgggatc aatgtgataa tcgccgttcc gaaaatggcg gcaatctggc ttttcataaa
    53641 ggtggagatc agcagcccca ttccagtggc aatgatgata tacagcagcg ccgccaaggt
    53701 gagcgtcagg aaactgcctt tatgcggtac gccaaacaca aacaccgaca ggccgcagag
    53761 caggaaaaag ttcagcatcc ccagcgcgat gtacggcagc tgtttgccaa gcaaaaattc
    53821 gctacgggtg gtgggggtca cgtaaaggtt gataatcgac cctagctctt tttcccgcac
    53881 cacgctaagg gcgcttagca ttgacgggat catcatcagc agaagcggga tcaccgccgg
    53941 aacaatcgct ggcaggcttt ttacgtccgg gttatagcga taacgcgtct caatgttcat
    54001 cagcccgctt tggctggcgg gtgtcgattg tcggctcgcc acatcctgaa gccaactctg
    54061 gtgcattgcc tgcacgtaac cttttaccgt ttcggcacga ctgggcatcg ctccgtcgat
    54121 ccagacgccg agttccacag gcgtaccacg cgcgatatcg cgcccgaaat tgggcgggat
    54181 ctcaatggcc accgtgatat cgcccgcacg catccgacga tcaagctcgt cataactggt
    54241 gagcggcggc tgttcgataa agtaacggga accggagagg ttgagtgtcc acgcctggct
    54301 actgacggtc tggtcgcggt cgagcaccgc aaagcgcagg ttttccacat ccatactgat
    54361 gccgtaaccc attatcagca tcaggatcac cgttcccatc agcgccagcg tcgaacgtac
    54421 tggatcgcgt cgcagttcca gcgcttcgcg gcggctgtag ctaaacagac ggcgcaggct
    54481 aaatccctga cgcggcgcgt gggtggtgtc gtgtaccacg ggcggcgctt cggcttcgtt
    54541 gctctgccct gccgcttcct gcaaataggc gataaatgcc tcttccagac tggcggctcc
    54601 gcgtttctca accagttcct gcggtgtacc gctggcaagc acttttccgg cgtgcatcag
    54661 tgagatgcgg tcgcaacgtt ccgcttcgtt cataaagtgg gtggagatga agatagtcac
    54721 tttgtcctgg cgcgagagat cgaccatcaa ctgccagaac atatccctcg ccaccggatc
    54781 gacaccagaa gtaggctcat cgaggattaa catctccggg cgatgaatca ccgccaccgc
    54841 cagcgaaagc cgctggcgaa tgccgagcgg caatgactcc ggcagaacat cttcaacgtc
    54901 gttgagctta aaacgctcgc tcatttcagc cactcgcgcg ggaatttccg cttccgggat
    54961 gtgaaacaaa cgggcatgta actccaggtt ttgccgcacg gtgagttcgt tatagagcga
    55021 aaacgcctgc gacatatagc ccacacgacg gcgggtatcg atatcttttg gatcaaccgg
    55081 ttgcccaaat agccacgcct caccttcgct ggcgggcagc agtccggtaa gcattttcat
    55141 ggtggtggat ttaccgcagc cgttcgaacc aaggaatcca aaaatctccc cgcgtggaat
    55201 gcggaaattg acgtgatcaa cggcaacgaa ggaaccaaaa cgcatggtca gatcgcgcgc
    55261 ttcgatggca atctctgcgt tttcaggttg atacggtggg atcactaccg cctgatgcgc
    55321 ctggcgttgc gcttgcggta acagattaat aaatgcttct tccagcgtgg cgctttgcgt
    55381 ttgctgccgc agctcttcgg cgctgccggt tgccagcact tctccggcat tcatcgctac
    55441 cagccagtcg aagcgttcgg cctcttccat ataggcggtg gcgaccagca cgctcatatt
    55501 gctctgccgc tggcgaatac tgtcgatcag atcccagaac tgggcgcggg agagcgggtc
    55561 aacccccgtt gttggctcat caaggatcaa cagttccggg tcgtggatta acgcgcagca
    55621 cagcccaagt ttttgcttca tcccgccgga gagtttacct gccggacgat cgcgaaacgg
    55681 tgctaacccg gtgctggtca gcagctcatt aattcgcgct tcccgctccg ctttatcgtg
    55741 accgaacagg cgagcgaaaa aatcgacatt ttcatacacc gacaaggtgt ggtagaggtt
    55801 tttgcccagc ccctgcggca tccaggcgat gcgcgggcag acgtctcggc gatgcttcgg
    55861 gtcgtgcata tcgccaccca gcaccaccac attgccctgc tcaatgacgc gggcaccgga
    55921 aatcagtgac aataaactcg acttcccgac gccatccgga ccaatcagcc cgaccatgca
    55981 acgggcctga atatcgagag tgatattgtt cagcgcaacg gtttttccat aatgctggct
    56041 cacgcccgcc agttgcgcga caggcgggac gggaaccagt tccagatgcg tcattgcggc
    56101 aacctcacca cgaggtcgtc aggccacgga agttcttcat tcacccgcac ccacgctacg
    56161 cccggtaaac cggttttgac atattccaga tgctgctgga gtaattccgg tgggatacgc
    56221 gctttgacgc ggaacatcag tttcagccgt tcatcgctgg tttcgacggt ttttggcgtg
    56281 aactgggcga cactggcgac aaaactgatg gttgcaggaa tacgcagatc tggcgcggca
    56341 tcgaggatca gccgggcttc accgcccagt ttcagcgtgc ccgcctgttc ggttggcagg
    56401 aagaaggtca tatagacgtc gctgagatcg accatattca gcacccgacc gcctgccgcc
    56461 agcacttccc ctggctcggc aacccgatac tgcacgcgtc cgtcacgcgg ggctttcagt
    56521 tcactgtcat cgatatctgc ggcaatgcgc cgttcagtgg cttgcgctgc ttcgacccga
    56581 gtttgcgcct gaatgatatt ggtgcgtgct gcttctatag ccgctttaga agccgaaacc
    56641 tgggctttcg ctgattccag cgcagctcgg gcgctctcag cggcggcgcg gtcgtcatcc
    56701 agctgttgcg cagaaatagc ccctcgttgg gcgagcgagc gggaacgcgt atgacgtttg
    56761 gcaacagagt ccagttcggc ctggcgttga ttgaccagcg actgtgcggc acgagtttca
    56821 ctttgtcgtt gctccagcaa agcctgcgcg gcggcaacgg cgctttgtgc ctctttgatt
    56881 tgcgcgatgg cttccagtcg ctgttcctgc aacacgcgag tatccatctt cgccagcact
    56941 tcgccttcgc gaacaaactg gccttctttc accagaatgg tgtcgatacg cccggcaatt
    57001 ttgctggcga tatccacttc cgtcgcttca atgcgcccat tgctgacagc aaagccttcc
    57061 ggcacacccg ccgggcgcaa cagccaccac gccacgacgg cagccaccgc cagtgccccg
    57121 acaacccacc acgccagatg gcgcttactc ttatccataa tcgacccgcc ataatccctg
    57181 taagcaaacg acgcgtgtta caactgccgc cgaacgacgt tcagcggatt cttccatccg
    57241 ggataagaag gaaacgacaa atacgaccag cgcgcagagc attgagcgcc aacggggaag
    57301 gcttagtgaa aacgggtagt aatgttgtca tcatcagcat tcctggccgt aaatgaaaca
    57361 cgcatactca taagtgtgat ctataaaact ggctgacaaa acggtatcca tcctgttgga
    57421 tcgcatttca cgcctgttca gttgcaggaa cagagcattc ctttttacgt tttatcaaca
    57481 gtctcaacca gatagtaaca ttaatattca tgttgtctat ggttcagagc tgtaacattg
    57541 tgcgccttct aatcaaaggc ataagtccat ttctgttttt ttcatgctgg taagacttcc
    57601 agggagtggt tatctctggc cctgcaccag ggttaatttg gcaattatgc atgctgaaca
    57661 ttcttccagc atgtttactg tcacgggcgt taagacctaa aactgtcccc gtttcaccta
    57721 tgcgttctct cccaccattt ccgttatgtg caacaacttg atttatacct aacatttgtg
    57781 aacatgctaa tagtgcttca agtttaggtc tgtaatctgt cgaaacagaa aaataacgat
    57841 taaaggatcg acaagagata gcccggctag ttttaaactt tttgccatta attatagcat
    57901 gatgcttttt attaataatt tccgctaatt cgaccggatc gagagcattg gtcatttcat
    57961 caatagagac agttatcggt cctataatgt aacgttttcg ctgatcatca aaagttatcc
    58021 catgatgatt tgccattatt ttgtatttgg ggtcatagaa acatacagga taatctttta
    58081 tcccgtcgta ggtatcctca tcaagtggtt tcatgcgcgt gaagttttgc atatagttac
    58141 cattgcaatt cgtttcatga tttccagcta atacatgaac attttcattg atactcatta
    58201 tgcgttttaa caaatcgatg ataaattgat cgccacagat attactaaat cgatcgccgg
    58261 tttggtcgcc aagaaacgta catggcgtga ctgcgtcttt attaataacc aaataaggct
    58321 gaagactttc taaaacatta tctcttctgt tcagtatact gattattgta ttaacattag
    58381 tgtcaagttg ctcattaaag ttctccctta gcaatacgtt gagaacttca tcttctatat
    58441 taagcaactg gctaaggaaa ttttctcctt cagccatgat tcccaaatgt cctgttgcaa
    58501 aaagcgcgta caaaactgca ccgactgagc catcggtatc gccaaaatag gtcggcctgg
    58561 tacttgtggg atcaacaggt gaacaattgg agaaataggt tctgtcagcg aactggggtt
    58621 ttataattgt ttcgaattta atatcaaata cattgtattg cgatatttcc cagttccaga
    58681 tgggtttgta cgactcagta actgcaacat cattagca
//
LOCUS       DAZPCS010000007        58574 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZPCS010000007 DAZPCS010000000
VERSION     DAZPCS010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58574
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.007"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..1101)
                     /locus_tag="ACHUQ7_000381"
     CDS             complement(<1..1101)
                     /locus_tag="ACHUQ7_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFR1133962.1"
                     /translation="MKQKITDYMDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPIFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQLPGFEDFFIAVDP
                     KTDLSAVTLPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDDVAPGTVIITETNVPH
                     KDNIAYFGNGDDEAHMVYQFSLPPLVLHAVQKQNVEALCQWAQSLSLPSSKTTWFNFL
                     ASHDGIGLNPLRGLLPESEILALVEALQQEGAL"
     gene            complement(1313..1660)
                     /gene="pspE"
                     /locus_tag="ACHUQ7_000382"
     CDS             complement(1313..1660)
                     /gene="pspE"
                     /locus_tag="ACHUQ7_000382"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFR1133963.1"
                     /translation="MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQ
                     QEHVQGAINIPLKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKELLSEMGYTHVENA
                     GGLKDIAMPKVKG"
     gene            complement(1703..1924)
                     /gene="pspD"
                     /locus_tag="ACHUQ7_000383"
     CDS             complement(1703..1924)
                     /gene="pspD"
                     /locus_tag="ACHUQ7_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFR1133964.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1933..2292)
                     /gene="pspC"
                     /locus_tag="ACHUQ7_000384"
     CDS             complement(1933..2292)
                     /gene="pspC"
                     /locus_tag="ACHUQ7_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFR1133965.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(2292..2516)
                     /gene="pspB"
                     /locus_tag="ACHUQ7_000385"
     CDS             complement(2292..2516)
                     /gene="pspB"
                     /locus_tag="ACHUQ7_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFR1133966.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2570..3238)
                     /gene="pspA"
                     /locus_tag="ACHUQ7_000386"
     CDS             complement(2570..3238)
                     /gene="pspA"
                     /locus_tag="ACHUQ7_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFR1133967.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3405..4382
                     /gene="pspF"
                     /locus_tag="ACHUQ7_000387"
     CDS             3405..4382
                     /gene="pspF"
                     /locus_tag="ACHUQ7_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFR1133968.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4502..5767)
                     /gene="puuE"
                     /locus_tag="ACHUQ7_000388"
     CDS             complement(4502..5767)
                     /gene="puuE"
                     /locus_tag="ACHUQ7_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HFR1133969.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINTLAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGECGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(5805..7085)
                     /gene="puuB"
                     /locus_tag="ACHUQ7_000389"
     CDS             complement(5805..7085)
                     /gene="puuB"
                     /locus_tag="ACHUQ7_000389"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFR1133970.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWVRYGNKQLELLDVNAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSHMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(7087..8574)
                     /gene="puuC"
                     /locus_tag="ACHUQ7_000390"
     CDS             complement(7087..8574)
                     /gene="puuC"
                     /locus_tag="ACHUQ7_000390"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HFR1133971.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVRIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHTFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(8724..9281)
                     /gene="puuR"
                     /locus_tag="ACHUQ7_000391"
     CDS             complement(8724..9281)
                     /gene="puuR"
                     /locus_tag="ACHUQ7_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFR1133972.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(9308..10072)
                     /gene="puuD"
                     /locus_tag="ACHUQ7_000392"
     CDS             complement(9308..10072)
                     /gene="puuD"
                     /locus_tag="ACHUQ7_000392"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFR1133973.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            10284..11702
                     /gene="puuA"
                     /locus_tag="ACHUQ7_000393"
     CDS             10284..11702
                     /gene="puuA"
                     /locus_tag="ACHUQ7_000393"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFR1133974.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGDLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYHVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            11851..12015
                     /locus_tag="ACHUQ7_000394"
     CDS             11851..12015
                     /locus_tag="ACHUQ7_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1133975.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            12005..13390
                     /gene="puuP"
                     /locus_tag="ACHUQ7_000395"
     CDS             12005..13390
                     /gene="puuP"
                     /locus_tag="ACHUQ7_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFR1133976.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            13524..13769
                     /locus_tag="ACHUQ7_000396"
     CDS             13524..13769
                     /locus_tag="ACHUQ7_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HFR1133977.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            14082..15725
                     /gene="sapA"
                     /locus_tag="ACHUQ7_000397"
     CDS             14082..15725
                     /gene="sapA"
                     /locus_tag="ACHUQ7_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFR1133978.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCNPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            15722..16687
                     /gene="sapB"
                     /locus_tag="ACHUQ7_000398"
     CDS             15722..16687
                     /gene="sapB"
                     /locus_tag="ACHUQ7_000398"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFR1133979.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKELFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            16674..17564
                     /gene="sapC"
                     /locus_tag="ACHUQ7_000399"
     CDS             16674..17564
                     /gene="sapC"
                     /locus_tag="ACHUQ7_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFR1133980.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            17564..18556
                     /gene="sapD"
                     /locus_tag="ACHUQ7_000400"
     CDS             17564..18556
                     /gene="sapD"
                     /locus_tag="ACHUQ7_000400"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFR1133981.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            18558..19364
                     /gene="sapF"
                     /locus_tag="ACHUQ7_000401"
     CDS             18558..19364
                     /gene="sapF"
                     /locus_tag="ACHUQ7_000401"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFR1133982.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            19432..19785
                     /locus_tag="ACHUQ7_000402"
     CDS             19432..19785
                     /locus_tag="ACHUQ7_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFR1133983.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            20153..20941
                     /gene="fabI"
                     /locus_tag="ACHUQ7_000403"
     CDS             20153..20941
                     /gene="fabI"
                     /locus_tag="ACHUQ7_000403"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFR1133984.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            21085..22212
                     /locus_tag="ACHUQ7_000404"
     CDS             21085..22212
                     /locus_tag="ACHUQ7_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HFR1133985.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETLSLHPISLSLLEKLLNSTRVNAQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            22280..24214
                     /gene="rnb"
                     /locus_tag="ACHUQ7_000405"
     CDS             22280..24214
                     /gene="rnb"
                     /locus_tag="ACHUQ7_000405"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFR1133986.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISSEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(24292..24395)
                     /locus_tag="ACHUQ7_000406"
                     /pseudo
     CDS             complement(24292..24395)
                     /locus_tag="ACHUQ7_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24449..26423
                     /gene="pdeR"
                     /locus_tag="ACHUQ7_000407"
                     /pseudo
     CDS             24449..26423
                     /gene="pdeR"
                     /locus_tag="ACHUQ7_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            complement(26405..26533)
                     /gene="ymiD"
                     /locus_tag="ACHUQ7_000408"
     CDS             complement(26405..26533)
                     /gene="ymiD"
                     /locus_tag="ACHUQ7_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFR1133987.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTGYSAKYIYSSTYARFR"
     gene            26571..26744
                     /gene="yciZ"
                     /locus_tag="ACHUQ7_000409"
     CDS             26571..26744
                     /gene="yciZ"
                     /locus_tag="ACHUQ7_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFR1133988.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            26834..27583
                     /locus_tag="ACHUQ7_000410"
     CDS             26834..27583
                     /locus_tag="ACHUQ7_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFR1133989.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            27852..28070
                     /gene="osmB"
                     /locus_tag="ACHUQ7_000411"
     CDS             27852..28070
                     /gene="osmB"
                     /locus_tag="ACHUQ7_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFR1133990.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(28196..28522)
                     /gene="yciH"
                     /locus_tag="ACHUQ7_000412"
     CDS             complement(28196..28522)
                     /gene="yciH"
                     /locus_tag="ACHUQ7_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFR1133991.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(28522..29259)
                     /gene="pyrF"
                     /locus_tag="ACHUQ7_000413"
     CDS             complement(28522..29259)
                     /gene="pyrF"
                     /locus_tag="ACHUQ7_000413"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFR1133992.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CDLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(29452..30621)
                     /gene="lapB"
                     /locus_tag="ACHUQ7_000414"
     CDS             complement(29452..30621)
                     /gene="lapB"
                     /locus_tag="ACHUQ7_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFR1133993.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(30628..30936)
                     /gene="lapA"
                     /locus_tag="ACHUQ7_000415"
     CDS             complement(30628..30936)
                     /gene="lapA"
                     /locus_tag="ACHUQ7_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFR1133994.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(31085..31849)
                     /gene="pgpB"
                     /locus_tag="ACHUQ7_000416"
     CDS             complement(31085..31849)
                     /gene="pgpB"
                     /locus_tag="ACHUQ7_000416"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFR1133995.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            32019..32609
                     /gene="ribA"
                     /locus_tag="ACHUQ7_000417"
     CDS             32019..32609
                     /gene="ribA"
                     /locus_tag="ACHUQ7_000417"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFR1133996.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(32673..35348)
                     /gene="acnA"
                     /locus_tag="ACHUQ7_000418"
     CDS             complement(32673..35348)
                     /gene="acnA"
                     /locus_tag="ACHUQ7_000418"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFR1133997.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMFIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELKVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGEPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(35512..35607)
                     /gene="ymiC"
                     /locus_tag="ACHUQ7_000419"
     CDS             complement(35512..35607)
                     /gene="ymiC"
                     /locus_tag="ACHUQ7_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFR1133998.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(35721..35888)
                     /gene="yciX"
                     /locus_tag="ACHUQ7_000420"
     CDS             complement(35721..35888)
                     /gene="yciX"
                     /locus_tag="ACHUQ7_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFR1133999.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(35891..36031)
                     /locus_tag="ACHUQ7_000421"
     CDS             complement(35891..36031)
                     /locus_tag="ACHUQ7_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFR1134000.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
     gene            complement(36350..37324)
                     /gene="cysB"
                     /locus_tag="ACHUQ7_000422"
     CDS             complement(36350..37324)
                     /gene="cysB"
                     /locus_tag="ACHUQ7_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFR1134001.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGPPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(37534..40131)
                     /gene="topA"
                     /locus_tag="ACHUQ7_000423"
     CDS             complement(37534..40131)
                     /gene="topA"
                     /locus_tag="ACHUQ7_000423"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFR1134002.1"
                     /translation="MGKALVIVESPAKAKTINKYLGCDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALELQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            40511..40762
                     /locus_tag="ACHUQ7_000424"
     CDS             40511..40762
                     /locus_tag="ACHUQ7_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFR1134003.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(40798..41847)
                     /gene="sohB"
                     /locus_tag="ACHUQ7_000425"
     CDS             complement(40798..41847)
                     /gene="sohB"
                     /locus_tag="ACHUQ7_000425"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFR1134004.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            42067..42825
                     /locus_tag="ACHUQ7_000426"
     CDS             42067..42825
                     /locus_tag="ACHUQ7_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFR1134005.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQINVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            42822..43412
                     /gene="cobO"
                     /locus_tag="ACHUQ7_000427"
     CDS             42822..43412
                     /gene="cobO"
                     /locus_tag="ACHUQ7_000427"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFR1134006.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(43452..44324)
                     /gene="rluB"
                     /locus_tag="ACHUQ7_000428"
     CDS             complement(43452..44324)
                     /gene="rluB"
                     /locus_tag="ACHUQ7_000428"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFR1134007.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGSRRSGGRNNG"
     gene            complement(44425..45045)
                     /locus_tag="ACHUQ7_000429"
     CDS             complement(44425..45045)
                     /locus_tag="ACHUQ7_000429"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFR1134008.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(45042..45923)
                     /gene="rnm"
                     /locus_tag="ACHUQ7_000430"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             complement(45042..45923)
                     /gene="rnm"
                     /locus_tag="ACHUQ7_000430"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFR1134009.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            46061..46105
                     /gene="trpL"
                     /locus_tag="ACHUQ7_000431"
     CDS             46061..46105
                     /gene="trpL"
                     /locus_tag="ACHUQ7_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFR1134010.1"
                     /translation="MKAIFVLKGWWRTS"
     misc_feature    46082..46175
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            46197..47759
                     /gene="trpE"
                     /locus_tag="ACHUQ7_000432"
     CDS             46197..47759
                     /gene="trpE"
                     /locus_tag="ACHUQ7_000432"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFR1134011.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYIADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            47759..49354
                     /gene="trpD"
                     /locus_tag="ACHUQ7_000433"
     CDS             47759..49354
                     /gene="trpD"
                     /locus_tag="ACHUQ7_000433"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFR1134012.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            49355..50716
                     /gene="trpCF"
                     /locus_tag="ACHUQ7_000434"
     CDS             49355..50716
                     /gene="trpCF"
                     /locus_tag="ACHUQ7_000434"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFR1134013.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLL
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            50728..51921
                     /gene="trpB"
                     /locus_tag="ACHUQ7_000435"
     CDS             50728..51921
                     /gene="trpB"
                     /locus_tag="ACHUQ7_000435"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFR1134014.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            51921..52727
                     /gene="trpA"
                     /locus_tag="ACHUQ7_000436"
     CDS             51921..52727
                     /gene="trpA"
                     /locus_tag="ACHUQ7_000436"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFR1134015.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            53107..53286
                     /locus_tag="ACHUQ7_000437"
     CDS             53107..53286
                     /locus_tag="ACHUQ7_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFR1134016.1"
                     /translation="MAEHRGSSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            53372..53872
                     /locus_tag="ACHUQ7_000438"
     CDS             53372..53872
                     /locus_tag="ACHUQ7_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFR1134017.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            53918..54424
                     /locus_tag="ACHUQ7_000439"
     CDS             53918..54424
                     /locus_tag="ACHUQ7_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFR1134018.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSIGGYV
                     FEQFEITCYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(54484..55122)
                     /gene="ompW"
                     /locus_tag="ACHUQ7_000440"
     CDS             complement(54484..55122)
                     /gene="ompW"
                     /locus_tag="ACHUQ7_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFR1134019.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            55362..55451
                     /locus_tag="ACHUQ7_000441"
                     /pseudo
     CDS             55362..55451
                     /locus_tag="ACHUQ7_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            55479..56222
                     /locus_tag="ACHUQ7_000442"
     CDS             55479..56222
                     /locus_tag="ACHUQ7_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFR1134020.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            56252..56791
                     /locus_tag="ACHUQ7_000443"
     CDS             56252..56791
                     /locus_tag="ACHUQ7_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFR1134021.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLSKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            56896..57294
                     /gene="yciA"
                     /locus_tag="ACHUQ7_000444"
     CDS             56896..57294
                     /gene="yciA"
                     /locus_tag="ACHUQ7_000444"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFR1134022.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(57334..58062)
                     /gene="tonB"
                     /locus_tag="ACHUQ7_000445"
     CDS             complement(57334..58062)
                     /gene="tonB"
                     /locus_tag="ACHUQ7_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFR1134023.1"
                     /translation="MTSITLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAP
                     AQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPK
                     PKPVKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALS
                     RNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRY
                     EPGKPGSGIVVNILFKINGTTEIQ"
     gene            58277..58573
                     /locus_tag="ACHUQ7_000446"
     CDS             58277..58573
                     /locus_tag="ACHUQ7_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFR1134024.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
BASE COUNT        14063 a        15144 c        14643 g        14724 t
ORIGIN      
        1 taatgcacct tcctgctgta atgcctcgac cagcgctaat atttcgcttt ctggaagcaa
       61 gccacgcagt gggttcaacc cgatgccatc gtgagaggcg aggaagttaa accaggtcgt
      121 tttactggag ggcaagctca gactttgcgc ccactggcac agcgcctcga cgttctgttt
      181 ttgcaccgca tgtaacacca gcggcggcag cgagaactgg tacaccatat gcgcttcgtc
      241 atcaccgttg ccaaagtaag caatgttgtc tttgtgcgga acattggttt cggtaataat
      301 cactgtacct ggtgctacgt catcaataat cgaccgtaac agcttgataa tcagatgtgt
      361 tttttccaga tggatgcagc ttgttcccgg ctctttccac ataaagccaa cggcatccag
      421 gcggacatac tcagcacctt tctcaaggta acagagtaaa acatccacca tcgccagcaa
      481 cacttcaggg ctacggtaat tcaggtcaat ttgatcgtca ctaaaggtgg tccataaatg
      541 gcgcgtggaa ttatcatcca tttgaaacgg cgttaatagc ggtaacgcac gcggaagggt
      601 gacggcactg agatcggttt tcggatcaac cgcaataaag aaatcttcaa aacccggaag
      661 ctgttgtaaa tagtttttaa accattcact ttttgcagac atatggttgc agacaaaatc
      721 aaacattaaa tgactgcatt caccgagttg ctgaatatcc tgccactccc ccgcttcact
      781 ggcgacctga tgataatcaa ttaccgaaaa tccatcatca gatgaccatg ggtaaaaagg
      841 taataaatga acatgtgaaa aaatgctttg cagccattga tggtagaact gattaaatat
      901 gggtagtggt tttaaatcat tactgtgaaa ttgatcggca taggtaatta acacgacatc
      961 actttcatcc cagtgttttt tacgtcgctg tgtaattaat cgtttcgcac tctcaagacg
     1021 cgttacaagt ttctgtaaat gagttgcggt aaatgttcca ccgtagattt cgtccatgta
     1081 atccgtaatt ttctgtttca tgttgccttt ccgtggtagc ggtcccatga ggcgagagac
     1141 tactccgcag aaaataaact gtcaattgag cacaagggtt ttagtcacgc aggaaatcat
     1201 tttttaccag ggaaaaagcg tatttgtgag ctgacgcaaa taatctatag ggatgtaaat
     1261 aaggtgtgac agaaaaaaaa cggcgcataa gcgccgctca tggtgaattc ttttaacctt
     1321 tgaccttcgg cattgcgatg tctttcaggc caccggcgtt ctcaacgtgg gtatatccca
     1381 tctcgctaag gagctctttt gcctgcccgg actggcgtcc ggcattgcaa tacactttca
     1441 cggtgtcgtt tttatccgga acggcggtgg caatgcgctc tttcacttcc ttcaggggaa
     1501 tattgatggc cccctgaacg tgctcttgct gatactgctc tggaacacga acatcgatcc
     1561 agtgttcagc ggcgaaaacg ggcagtgaaa acaccagtgc cagagcaagt aagccttttt
     1621 ttaaacatgt aagcgtcctt ctatggatga aaattgccat ccattatacg cctgagattt
     1681 aacaattttt gcgaaactcc cctcaccttt tataacgctg tgccagttta ttagcagccc
     1741 gacttaacag cggttccagc gccacagcca gcaacatttt cagcggtcgg cgagcaactg
     1801 attttatcgc ccagcccgcc acacccgccg ggccatagcg cagtgcggta agaagtacca
     1861 gcttgcctgc taatttgaaa ccaagcttca ccttttgccc ggcctgttgc cagcgagtat
     1921 tcataacttt cctcacagtt gacggaaacg gctacgtaac gtgaaagtat cggaagtgac
     1981 ataacgttcc atctcgcgta aacgcgtttc acttgccgcc agttcacggt cgacttcatc
     2041 cagcaattcg ctgctggaag gtagctgctc accaaaggcc atgttgtccg gcattggatc
     2101 aagcgcaaat gacaaaatga tgtaagcaac cagggtaaac agcgccagac cgaaaaaaat
     2161 cgacagcacc accaggatac gcaccagttt taccggtaca tcaaaatagt tggcaatccc
     2221 ggcgcagacg ccgcggacca tgccctgctg tggaatacgc cataactttt tattgagatt
     2281 aatgcccgcc attagcgatc cctccagttc ggatgttctg catcaagaat agattccagc
     2341 gcctgaatac gttcgcgcat ccgttttgct tcatcaacca gttgcgctaa tcgctgctgc
     2401 tcactttgcg acaattcact gcgaccagaa cgattgctgt aatgcagcca taaccagatc
     2461 ggtaaaacaa acagcacaaa aatggttaac ggaatagcca gaaatagcgc gctcataagt
     2521 actccttaga cgatgagcgg cggcgcgttc ggacgccgcc tggatgttat tattgattgt
     2581 cttgcttcat tttggctttt aattgtgcca gttgttcgct gattgcatca tcggctttca
     2641 gttcggcaaa ctgatcgtcc agcgattttt gtttaccgaa gctgtggctt tctgcttccg
     2701 cttccatctg gtcaatacga cgttcgaaag attcgaaacg agccattgct tcatccagtt
     2761 tgccactgtc cagctgacga cgcacatcgc gcgacgagtt tgccgcctga tgacgtaaca
     2821 tcaatgcctg ctggcgagcg cgtgtttcgc tcaatttgtt ttccagctcg ccaatctctt
     2881 tcttcatgcg tgccagcgta tcgtccacca gcgtcacttc atgttccagg gacttaatca
     2941 gatcggtcag tttctgtttt tcaattaacg ctgcacgtgc cagatcctct ctctctttca
     3001 gcagcgccag ttcggctttt tcctgccatt caacttcacg cgccgacgct tgttcaatac
     3061 ggcgagtcag ctgtttcttt tctgccaacg cacgcgccga agtagaacgt acttcaacca
     3121 gtgtatcttc catctcctgg atcatcagac gcaccagttt ctgtggatct tccgctttct
     3181 ctaacagagc gttgatgttg gcattcacga tgtcggcaaa gcgagaaaaa atacccataa
     3241 tgttgtcctc ttgatttctg cgttcaggat tgtcctgctg aactgttaat acaatttgcg
     3301 tgccaatttt ttatcttttt gatttataaa gatctgattg aagaatcaac agcaacatgc
     3361 caggatgaat tagcgaatta cactaacaag tggcgaattt catcatggcc gaatacaaag
     3421 ataatttact tggtgaggcg aacagctttc tcgaagtgct ggaacaggtt tcgcatctcg
     3481 caccgctgga caaaccggtg ctcatcatcg gcgaacgcgg caccggtaaa gagttgattg
     3541 ccagccgcct gcattatctc tcctcccgtt ggcaagggcc gtttatttcc cttaactgcg
     3601 cggcgttaaa tgaaaatctg ctggattccg aactgtttgg tcacgaagcg ggggcgttta
     3661 ccggtgcgca aaaacgtcat cctgggagat ttgaacgtgc cgacggcggt acgctatttc
     3721 ttgatgaact cgctacggca ccgatgatgg tgcaggaaaa attattgcgt gtgattgagt
     3781 acggtgaact ggagcgcgtt ggcggtagcc agccgttgca ggtgaatgtg cggttggtat
     3841 gcgcgacgaa tgccgatctc ccggcgatgg tcaatgaagg tacttttcgc gctgacctgc
     3901 tcgaccggct ggctttcgat gtagtgcaac tgccgcccct gcgcgagcgc gaaagcgaca
     3961 ttatgctgat ggcagaacac tttgccattc agatgtgtcg ggaaatcaag ctgcctctgt
     4021 tcccggggtt tacggagcgc gccagagaaa cattgctgaa ttatcgttgg ccgggaaata
     4081 ttcgtgaatt gaaaaacgtg gtggaacgtt cagtgtatcg ccacggcacc agcgattatc
     4141 cgcttgatga catcattatt gatcccttta aacggcgtcc acctgaagaa gccatcgccg
     4201 tttcagaaaa tacctcgctt ccgacgctgc cgctggattt acgcgagttt cagatgcagc
     4261 aggaaaaaga gttgctgcaa ctcagtttgc aacaggggaa atataaccag aagcgcgcgg
     4321 ctgaattact ggggttaact tatcatcagt tccgcgcgtt gttgaaaaag caccagattt
     4381 agcgaagttg agcaatacaa cttgccggat gcggcgtgaa tgctttattc ggcatacata
     4441 atcaatacgt cgtttgcagg cctgataagc gcagcgcatc agacattatt gcgttgggct
     4501 attaatcgct cagcgcatcc tgcaaaattt tcattgccgc atcgaattgc gcatccggga
     4561 tggtcagcgg atacaggaag cgaatcacgt tgccgtatgc gccacaggtc agcaggagca
     4621 gcccctgcgc cagcgcgcgt tgctggattt tctgtgcaat cgccgctgac ggctcgcccg
     4681 tttgcggatc gttaaactct gccgcaatca tcgaccccag gccgcgtacc gcagcaatgg
     4741 ccggaacgct ttctttggca tcaatcaacg tgtttttgag acgctggccc agttgattcg
     4801 cgcgttcgca gagtgattct ttgtcgataa tattgagcac cgcgtgcgcg gcagccaccg
     4861 ccagcgggtt cccggcgtag gtgccaccca acccgcccgg cgcgggcgcg tccataatat
     4921 tcgcgttacc gaccacgccc gaaagcggca tcccgcccgc gaggcttttc gccatcgtca
     4981 ttaagtccgg cttatcggcg taatgatcca tggcaaacag tttaccggta cgcgcaaagc
     5041 cgctttgcac ttcatcggca atcatcacaa taccgtgctc gtcgcacagg cggcgaatgg
     5101 cggcaaccag ctcttttggc gcaacgttga aaccgcactc gccctgcacc ggttcgaaaa
     5161 taatggccgc gacctgcttc gcttcgatgt ctgatttaaa caagcgttcg atggcgtcga
     5221 gggagtcctg tgttgaaatg ccgtgtaaat ctgacggata aggtacgtga tacaccgaac
     5281 cagggaacgg gccgaagccg attttgtacg gcgcaacttt tccggtcagc gccatggtca
     5341 tatatgtacg accgtggaag ccaccgctaa acgcaatcac gccaggtcgt ccggtatggg
     5401 cgcgagcaat tttcaccgcg ttttccaccg cttccgcgcc ggtagtgaag aatgcggttt
     5461 tagcctgccc gctcactggg gcaagggtgt taattttctc cgccagcgtg acgtagcttt
     5521 cgtacggcac aatctgatac gccgtgtggg taaactgttg caattgctgc tccaccgccg
     5581 cgaccagatc agggtggcga tgcccggtat tcagcaccgc aatgcctgcg gcgaaatcga
     5641 tgtattcgtt gccctctacg tccttcagcg tagcgttttc tgccgactgg gcgaagaagt
     5701 tacacatcac gccaaccccg cgtggggtag cagaaagacg acgctgatgg aattcattgt
     5761 tgctcatgtt tcacaccttt tgactcttca ctgttaatcg gaaattaaac gcccagacga
     5821 tcgcgcaggc tgtaataagc cgcgcccatc gcggtaaatg gcacgcgcag cgtgcgcccg
     5881 ccggggaacg ggtaatgcgg cagattggcg aaggcatcga aacgctcggc gtcaccgcgc
     5941 agcagttcag caatcaaacg cccggcaaga tgggtacagg tgacgccgtg gccgctgtaa
     6001 ccctgcatgt aatagatgtt ggtatcaagg cggccgaact gcggcatgtg cgacaaggtc
     6061 agcaggaagt tgcccgtcca gcggtaatca attttcacgc ccttcaactg agggaaggtt
     6121 tttagtagtt tcggtaccac aagacgctca acgtcatccg ggttgcgtgc accatacact
     6181 acacccccgc cgtacagcag gcggttgtcg gcggtaagac ggtagtaatc aagcagatag
     6241 ttacagtctt ccacacagta gtttttcggg atcagcgaac gggctaaatc ttccgacagc
     6301 cgttcggtgg tgatcacctg cgtgccgcac ggcatgctgc gtttcgctag ttccggctct
     6361 actttatcgc ccagatacgc attcccggcg acaatcacat acttcgccgt cacctgacca
     6421 ttggcagtcc gaaccacggc tggcgtggtg tgctggatct gcgtcacggc ggaaagctca
     6481 tacacgcgcc cgccgttcag gcggatggca tcggcttcac caatggcgag gtttagcggg
     6541 tgaatatgcc caccgctgtg atccagcagc gcaccagtgt agcgatcgct ggcgacctcg
     6601 cggcgaatag cgttgacgtc cagcagctcc agctgtttat tgccgtagcg tacccagttc
     6661 tctttctgct cttcgagtgt ggcgagttgt ttatcattca tcgccacaaa cagaccgccg
     6721 gggcggtagt cgcagtcaat ctgataacgt ttgatacgtt cgcggatgat ctcgccaccc
     6781 tcgaacatca tgctgccgag catacgggcg gtgtccatgc cgtagctttt ttcgatcacg
     6841 tcgatgtcgc ggctgtagga gttcacaagc tgcccgccat tgcgcccgct tgcgccaaag
     6901 ccgatgcgtg aggcttcgag aaccactacg tcaaagcccg cttccgccag atgcagcgcg
     6961 gaggagagcc cggtatagcc gccgccaacc acgcaaacgt cgcaggtgat tgactcattc
     7021 agtgtgtcga atggtgcata tttattcgca ctggcggcgt agtaactgct ggtatgttcg
     7081 gtcatttcag gcctccaggc ttatccagat ggttttcagt tcagtgaatt tttcaagggc
     7141 atgcagggat ttgtcgcgac cgttgccgct ctgcttatag ccgccaaacg gcacggtcat
     7201 atcgccgtcg ttgtagttat tgacgaagac ggaaccggct ttcaggcgtc ggctcatgcg
     7261 gtgcgcgcgg gagaggtcgc gcgtccatac cgccgcgcca aggccgtact ggctgtcgtt
     7321 ggcaagctgc agcgcctgat cttctgatgt gaaacgggtg accaccagca ccggaccgaa
     7381 aatctcttcg cggcttaagg acgcattcgg gtccacatcc acgaagatgg tcgggccgat
     7441 ggccgctgcc agcccggcgt tgcgaccatc cagcagcagt tgccccttgc cttcgccttc
     7501 acgaataaag gtgtgtaccg agtcagcgtg agcgctgtcg attaaggtgc ccatggtggt
     7561 tgcgggatca agtggatggc ccggctgcca gttttgcgcc tgctgtttta acagggctaa
     7621 gaattcatcg gcgatgctct cttccagtaa caaacgcgtt ccggcgatgc gcacctggcc
     7681 ctggttgtag aagatgcctg cggcggtggc gcttgccgct ttttgcagat ccggacaatc
     7741 ggcgaaaacg atgttggcgc ttttgccgcc cgcttccagc cagacacgtt tcatgttgct
     7801 atcacccgcg tctttcagca gttgtttacc cgtacgcgtg gagccggtaa aggcgatggc
     7861 atcgatatcg ttatgacgcg aaagcgcctg ccccgcttca tgaccaaaac ccgtcaccac
     7921 gttcaacaca ccatccggca agcctgcttc tttcgccagc cccgcgagac gaatcgcact
     7981 gagcggtgat ttttcagacg gttttagaat cacgctgttt cccgccgcca gcgccgggcc
     8041 gagtttccag caagtcagca acagcgggaa gttccacggc acgatggcgg caatcacgcc
     8101 gaccggttca cgcacgatca tcgccagctc atggctactg gtggtcgcca cttcgccata
     8161 cactttgtcg atcgcttcgg cgtaccagcg aatggcgcgc gccgcgccgg gaatatcatc
     8221 acgcagactg tgacgaatcg gtttgccggt gtcgagagtt tccagcagtg ccagctcttc
     8281 ggcgtgggct tccattaaat cggcgagttt attcagtacc gctttacgtt tagccggaga
     8341 agagagtgac cagtcgccgc gttcaaatac gccgcgtgct gcgctcaccg cacggtcgat
     8401 atcgacgctc ttgccgcggg caattttcgc cagcggtgcc tgggtgaccg gatcaacggt
     8461 ttcaaaggtt tcattttccg ccgcagcagt atattcaccg ttaataaata agcggttttc
     8521 aatggcgaga cttaacgctt tatcctgcca gtaagccaga tgatgaaaat tcattatgac
     8581 tcctgtttca cgtctatcag atatatgcag tttgtgaacg tcagggcgca gagatccccg
     8641 ggctgcatgc ccgtttattt ctggcgacat tctttatgga ttgccgggcg cattgcgctt
     8701 acccggctta cagaacaaaa agattaaaac gtggtgggcg tatgggcgct gataattcgg
     8761 caaatacctg ccgacgtatt actgaaactg tgcgggatgc cggtattaat ggcataactt
     8821 tgccccgcga cgaggtggta atcctgacca ttaatcgtca gaacaatttc accttccagt
     8881 actgtgccta tttcctcacc ctgatgctta attctttccc cagtggttgt gcccggctgg
     8941 tacgtttcaa agatcatcgc cagcgtgcga ttcgggttac cgttatgaac cagcttcatt
     9001 gacacaccct gactgcccat ctcaattaag tcgtcctgat taatgacgac ctgcggctca
     9061 tcaggttttt ccggctcgga aaagaattcc gagagtgaca gaccatacac cttcagcagc
     9121 ttttgcagcg tactgatggc agggctgact ttatcttgtt ctatcgtact gatagcactg
     9181 tgagtcagcc cggagagttc ggcggcacga cgttgtgaaa gcccctgctg ctggcggatt
     9241 tccgacaagc gttttcctgg cgccagtccc tcatcactca tatttgcatt tccttaaact
     9301 gtagtggtca gagtcgctgt ttttcagcga tatggtgctg ccaagcggtg ataaagccct
     9361 cgaacaatat acgcgaaagc gcgtactcgc tactgttcca ttccgggtgc cactgtacgc
     9421 ccagcgcaaa aggatgattg atgacgctaa ccgcctcaac taagccatcc ggcgagcgag
     9481 cttcaacacg caaccgtggg ctaacgacct tcgccccttg tccatgtaga gagtttaccc
     9541 aaaagttgct acattcaggt aacaacgcag acagtaatcc cccctcttca acctgaactt
     9601 catgcgacgg tgcatattgc tgttccaccg gcagttcggg atcttcccga tgttccagca
     9661 attcaggctg ttcgcacagc ttgcgatgca acgaaccacc ggttgccacc accagttctt
     9721 gtaaaccccg gcagatggcg aaaatgggga tgcgcctttc gagtgcggca ttaattatcg
     9781 ccatgctcag aagatcacgc ccgggatcgg cgtcaggctc atcgccgttt tcaccatata
     9841 ggtgcggctg cacattgctg ggactactag gaagataaat gccatcgagt ttcggcaaaa
     9901 gttgttcaag taacgacggt tccgccagcg catgtggtag cgcaattggc aagccgcctg
     9961 cgtggatgat ggcattcagg tacttttctt gcagagtctg ggtcgcatga cccttaagcc
    10021 tgttcctgca cattacgaca ccgataaccg gattgttcat tatattttcc atgctcaatc
    10081 tcacaaaatg gactaaatta tcgccattac tgcgctttaa atgaccttta tgttcaatat
    10141 tttttcaatc tagcagtgga ttttcatttt tgcaaactca atttaacatt tgacaaacat
    10201 ttggtttgca tacagattcg aatgatggtc attatatttt acgctttgat aacgagcgga
    10261 aaacaaacca aaggcgaaga atcatggaaa ccaatatcgt tgaagtagag aactttgttc
    10321 agcagtcaga agagaggcgg ggtagcgcct ttacgcagga agtgaaacgc tacctggagc
    10381 gctacccgaa tacgcaatat gttgatgttt tacttactga tttgaatggt tgcttccgtg
    10441 gaaaacgtat ccctgtttca agcctgaaga agctcgagaa agggtgttat ttcccggcct
    10501 cggtatttgc aatggatatt ctgggcaacg tagtagaaga agcaggtctg ggtcaggaaa
    10561 tgggcgagcc ggatcgtacc tgtgttcctg ttctcggttc cttaactcct tctgccgccg
    10621 atccagagtt tatcggtcag atgctcctga ccatggtcga tgaagatggc gctccctttg
    10681 acgttgagcc gcggaacgtt cttaaccgcc tgtggcagca gctgcgccag cgcggattgt
    10741 tcccggttgt agcggtagag ctggagttct atttactgga tcgccagcgc gacgctgaag
    10801 gggatctgca accgccctgc gcgcctggca ccgatgaccg caatacacaa agccaggttt
    10861 actccgttga taacctcaac cacttcgccg acgtgctcaa tgatattgat gaactggcgc
    10921 agttgcagct gattccggca gatggcgcgg tcgctgaggc ctcgccgggt cagtttgaaa
    10981 tcaaccttta ccatactgat aacgtgctgg aagcctgcga tgatgcgctg gcactaaaac
    11041 gcctagtgcg tctgatggca gaaaagcata agatgcacgc cacttttatg gcgaagccgt
    11101 atgaagagca cgcgggcagc gggatgcata tccatatcag tatgcaaaat aatcgtggcg
    11161 agaatgtgct ttctgacaca gaaggcgaag attcgccgct gctgaaaaag atgctcgccg
    11221 ggatgattga cctgatgccg tcgtcgatgg cgttgctggc accaaacgtg aactcgtatc
    11281 gccgcttcca gccgggaatg tatgtgccga cgcaggcgtc gtggggccat aacaaccgca
    11341 ccgtcgccct gcgtattccg tgcggtgacc gtcataatca ccgcgtggaa tatcacgtgg
    11401 cgggtgccga tgccaaccca tatctggtga tggcagcgat ttttgccggt attttgcatg
    11461 gccttgataa cgagctgccg ttgcaggaag aagtcgaagg caacgggctg gaacaggaag
    11521 gcttaccctt cccgattcgc cagagcgatg ccctgggtga gtttatcgag aatgatcacc
    11581 tgcgccgcta tttaggcgaa cggttttgcc atgtgtatca cgcctgtaaa aatgatgagc
    11641 tgttgcagtt tgagcgcctc atcacagaaa cagaaattga gtggatgttg aaaaacgcgt
    11701 aattcccctt gcggggttgt ttcatcggcc tgagcggagc caggcctgac atttgctggt
    11761 aatccagaca tttcccggat gtcgcgttat ggagcgcagg cggcaacggg cttccgttaa
    11821 cgattaagac tgtgacgagg aaatgagatg atgccgatga ttaaatcccc gcatggcgag
    11881 ggaggctgcg tatgcgcccc tcctgctact gactggactc cgcccccttt gcttcccttg
    11941 ctaaacaggt ttgatttcag gtcgacacga ccgcaaacct tattacgcag gggaggcagc
    12001 aattatggct attaattcac cactgaatat tgctgcgcaa cccggcaaaa cccgtctgcg
    12061 aaaatcactg aaattgtggc aggtggtaat gatgggtctg gcctatctca cgccgatgac
    12121 cgtatttgat acttttggta ttgtctccgg cattagcgac ggtcacgttc cggcgtccta
    12181 tttgctggcg ctggcgggcg tgctgtttac cgctatcagc tacggcaaac tggttcgcca
    12241 gtttccggag gccggttcgg cctataccta cgcgcaaaag tcgattaacc cgcacgtcgg
    12301 atttatggtc ggctggtcat cactgctgga ttatctcttt ttgccaatga tcaacgtttt
    12361 gttggcgaaa atctatctct ccgccctctt cccggaagtg ccgccgtggg tgtgggtggt
    12421 aaccttcgtc gccattttaa ccgccgcgaa tctgaagagc gtcaacctgg tcgctaactt
    12481 caataccctg tttgtactgg tgcaaatctc catcatggtg gtgtttatct tcctggtggt
    12541 tcagggactg cataaaggag aaggcgttgg caccgtctgg tcacttcagc cgtttatcag
    12601 cgagaacgcg cacctgatcc cgattattac cggtgcgacg attgtctgtt tctcgttcct
    12661 cggtttcgat gcggtgacca cgctttcgga agagacgccg gacgccgcac gcgtgatccc
    12721 gaaagccatc ttcctgacgg cggtatacgg cggcgttatc tttatcgcgg cgtcgttctt
    12781 tatgcagctg ttctttcccg atatcagccg ctttaaagac ccggacgccg cactgcctga
    12841 aattgcgctg tacgtcggcg gcaagctctt tcagtcgatt ttcctctgca ccacgtttgt
    12901 gaacacatta gcgtctggcc tggcttcaca tgccagcgtg tcgcgtctgc tgtatgtgat
    12961 ggggcgtgac aatgtgtttc cggagcgcgt gtttggctat gtgcacccaa aatggcggac
    13021 tccggcactc aacgtcatta tggtcgggat tgtcgcgctg tcggcgctgt tctttgattt
    13081 agtcaccgcg acagcactga ttaacttcgg tgctctggtg gcgtttacct tcgtgaatct
    13141 gtcggtgttt aatcatttct ggcggcgtaa aggaatgaat aaaagctgga aggatcactt
    13201 ccactatttg ctgatgccgc tggttggcgc gctgacggtg ggtgtgctgt gggttaacct
    13261 agagtcaacg tcactgacac tcggtctggt atgggcttcg ttaggcggcg catatttgtg
    13321 gtatctgatc cgccgctatc gcaaagtgcc gctgtacgaa ggtgacagaa cgccggtgag
    13381 cgaaacgtaa tagccggatg cgcggcgcat tatcctgcct gagggtaacg cgccgcgcca
    13441 ttaactataa aaagatgtat gacccggatc acgagtgcta tcatccgccc ttatgcttag
    13501 cgcattaacc ccaggggcaa ataatgaacc acgatatccc gctaaaatat tttgatattg
    13561 ccgatgagta cgcgaccgaa tgcgcagagc ctgtcgcaga ggctgaacgc acaccgctgg
    13621 cccactactt ccagttactg ctcacccgtt tgatgaacaa tgaagagatc agcgaagaag
    13681 cccaacatga aatggctgct gaagcgggga ttaatcccgt acgcattgat gagatcgcag
    13741 agtttttgaa tcaatggggt aatgagtaat tatttaattg cagacgatcg gtcagtatcg
    13801 ttgtccggat aaagcgttca cgccgcatcc ggctaacaat gacacgcact gcagcgtgct
    13861 ttaatgggtt tctcacgatc gtaccgctct gctcctcgct aagttgttct gtgagttata
    13921 gttatagaga aatcacgggt acgaatgttg aacagatggc gacgccacct gttcaacgac
    13981 caaccacacg gccctcatta cgacccctga acacaaacgg taatggcaaa tgtcattaaa
    14041 tattagtaag atagcctctt tacataaaat cccttaatat tatgcgccag gtattatcgt
    14101 ctcttttggt gattgctgga cttgtgagtg gtcaggcaat cgccgcgcct gaatcccccc
    14161 cgcatgctga tatccgcgac agcggttttg tctattgcgt cagcgggcaa gtcaacacct
    14221 ttaacccatc caaagcgagc agtgggttaa ttgtcgatac ccttgccgcc cagttttatg
    14281 atcgactgct ggatgtcgat ccctatacct atcgcctgat gccggaactt gccgaaagct
    14341 gggaagtact cgacaacggc gcgacctatc gcttccacct gcgtcgcgat gttccgtttc
    14401 aaaaaaccga ctggtttact cccactcgta aaatgaatgc cgacgatgtg gtgtttacct
    14461 tccagcgaat ttttgaccgc aacaacccgt ggcataacgt caacggcagc aacttcccct
    14521 acttcgacag cctgcaattt gccgataacg tgaaaagcgt ccgcaaactg gataatcata
    14581 ccgttgagtt ccgactcgcc cagccggatg cttctttttt gtggcacctc gcaacccatt
    14641 atgcttcggt catgtcggca gaatatgccc ggaagttaga gaaagaagat cgccaggagc
    14701 aactcgaccg tcaaccggtc ggcaccggtc cgtatcagtt gtcggaatac cgcgccgggc
    14761 aatttattcg cctacaacgt catgatgact tctggcgcgg taaaccgtta atgccgcagg
    14821 tagtggtgga tttaggctcc ggcggcaccg gacgtctgtc gaaactcctg accggggaat
    14881 gcgacgttct ggcctggcct gctgccagcc agctatccat tttgcgtgac gacccgcgct
    14941 tgcgtttaac gctgcgtcct gggatgaacg tcgcctatct ggcatttaac accgccaaac
    15001 cgccgctaaa taatcccgct gtccgccatg cactggcact ggcgattaat aaccagcgcc
    15061 tgatgcaatc catctattat ggtacggctg aaacggcggc ctctatttta ccgcgcgcct
    15121 cgtgggccta tgacaacgag gctaaaatta ctgaatacaa tccggcgaaa tcgcgcgaac
    15181 agttgaaggc gttggggctg gaaaacttaa cgctgaaact gtgggtgccc acacgttcgc
    15241 aggcgtggaa ccccagtcca ctgaaaactg ccgaactgat tcaggcggat atggcgcagg
    15301 ttggcgtaaa agtggtgatt gtgccggtag aaggtcgctt tcaggaggcg cggttgatgg
    15361 atatgagcca tgatctgacg ttatccggtt gggcgacgga cagtaacgac ccggacagtt
    15421 tcttccgtcc gttactgagc tgcgcggcaa ttcattcaca gaccaacctc gcccactggt
    15481 gcaatccgaa attcgacagc gtgttgcgta aggcgctctc ctcgcagcag ctggcagcgc
    15541 gtattgaagc ctatgacgaa gcgcagagta ttctggcgca ggaattacct attctgccgc
    15601 tggcgtcgtc attgcgtttg caggcctatc ggtacgatat caaaggtctg gtacttagcc
    15661 cgtttggtaa cgcctccttt gctggggtgt atcgcgagaa acaggatgag gtgaaaaaac
    15721 catgattatc ttcaccttac gccgcatttt gttattgatt gtcaccttgt tcctgctgac
    15781 cttcgttggc ttcagcttga gctatttcac tccacacgcg ccgttacaag gcgcgtcact
    15841 gtggaatgcc tgggtgttct ggtttaatgg cctgatccac tgggattttg gtgtttccag
    15901 cattaacggt caaccaattg ccgaacagtt aaaagagctc ttcccggcga cgatagaact
    15961 gtgcatcctc gccttcggtt ttgcgctgat tgttggcatc cccgtgggga tgattgccgg
    16021 gattacgcgc cataagtggc aggataactt gatcaacgcc atcgccttac tgggcttttc
    16081 aatcccggtg ttctggctgg cgcttctgtt gacactgttt tgttcactca cgctaggttg
    16141 gttgcccgtt tcagggcgtt tcgatctgct ctacgaagtg aaaccgatta ccggttttgc
    16201 gttgattgat gcctggcttt cggactcacc atggcgggat gaaatgatca tgagcgcaat
    16261 ccgccatatg atattgcccg tgatcaccct gtcggttgcg ccaacaactg aagtgatccg
    16321 cctgatgcgt atcagcacta ttgaagtgta tgaccagaac tatgtaaaag cggcggcgac
    16381 ccgcggtttg tcgcgcttta ctattttgcg tcgccacgtt ctgcataacg cgttaccgcc
    16441 ggttattcct cgcctgggct tacagttttc caccatgctg acgctggcga tgatcaccga
    16501 aatggttttt agctggccgg gtttagggcg ctggttaatt aacgccatcc gccagcagga
    16561 ttacgcagcc atttccgccg gagtgatggt gtgtggctca ctggtcatta ttgttaacgt
    16621 gatttctgat attttgggtg ccatggctaa ccctctgaaa cataaggaat ggtatgcctt
    16681 acgatagcgt atacagcgaa aagcgcccgc cgggcacgct gcgtaccgcc tggcgcaaat
    16741 tttatagtga cgcctctgcg atggtcggcc tgtacggctg cgcgggactg gctgtactgt
    16801 gtatttttgg cggctggttt gcgccttacg gcatcgacca gcaatttctc ggttatcaat
    16861 tattgccgcc atcatggtcg cgctatggcg aagtttcttt cttcctgggg actgacgacc
    16921 tcggacgtga tgttttaagc cgtttactga gcggagctgc gccaaccgtg ggtggcgcat
    16981 ttgtggtgac gcttgccgcg acgatctgtg gcctggtact gggcaccttc gccggggcga
    17041 cacacggcct ccgctcagcg gtgcttaacc atattctgga taccttactg gcgatccctt
    17101 cgctgctgtt ggcaattatc gttgtagcgt ttgccggacc gagtttgtcc cacgctatgt
    17161 ttgctgtctg gctggcattg ctgccgcgta tggtacgttc gatttacagc atggtgcatg
    17221 acgaactgga aaaagagtac gttatcgccg cccgtctgga tggcgcatca acgctgaata
    17281 ttctctggtt tgctgtgatg ccaaacatca ccgccgggct ggtcaccgag atcacccgcg
    17341 cactgtcgat ggcaattctc gatatcgccg cgctgggctt tctcgatctc ggcgcacaac
    17401 tcccctcgcc tgaatgggga gcgatgctcg gtgatgcgct ggaactgatt tatgtcgccc
    17461 cgtggactgt catgctgcca ggtgcggcaa ttatgattag cgtgttgttg gttaacctcc
    17521 ttggcgatgg cgttcgtcgt gcgattattg cgggggtgga ataatgccat tactcgatat
    17581 tcgtaacctg accattgaat ttaaaaccgg tgatgagtgg gttaaagccg tcgaccgggt
    17641 aagtatgacc ttaaccgaag gtgaaatccg cggtcttgtt ggtgaatccg gttccggcaa
    17701 gagtttgatt gcgaaagcga tttgtggggt gaataaagat aactggcgtg ttactgctga
    17761 ccgtatgcgt tttgatgata tcgatttgct gcgtctctcc gcacgcgaac ggcgcaaact
    17821 ggttggccat aacgtgtcga tgattttcca ggaaccgcag tcgtgtcttg acccttcaga
    17881 acgtgtgggt cgccagttga tgcaaaacat cccagcctgg acctataaag gccgttggtg
    17941 gcagcgtttt ggctggcgca aacgccgtgc cattgaactg ctgcaccgcg tggggattaa
    18001 agatcacaaa gatgcgatgc gcagttttcc ctatgagttg accgaaggtg aatgtcagaa
    18061 agtgatgata gccattgcac tggcgaatca accgcgtctg ctgattgctg acgaaccgac
    18121 aaactcaatg gagccaacaa cccaggcgca aatctttcgc ctgctgacgc gtctcaacca
    18181 aaacagcaat accactattt tgcttatcag ccatgactta caaatgctta gccaatgggc
    18241 ggataaaatt aacgtgcttt actgcggtca aacagtggaa accgcgccga gtaaggagtt
    18301 ggtgacgatg ccacatcatc cttataccca ggcgctgatc cgcgcgatac cagacttcgg
    18361 cagcgcgatg ccgcataaaa gtcgcctcaa tactctaccc ggcgctatcc cattgctgga
    18421 acagttaccg attggttgtc gtctggggcc acgttgcccg tatgcacaac gagaatgcat
    18481 tgtgacgcca cgtttgacgg gggcgaaaaa tcatctctat gcctgtcatt tcccgctgaa
    18541 catggagaaa gagtgagatg atcgaaacgc tgcttgaagt gcgtaattta agtaaaacgt
    18601 tccgctaccg gaccggttgg tttcgtcgtc agaccgtaga agcggtaaaa cccttgagct
    18661 ttacgctacg tgaaggccag acactggcga ttattggcga gaatggttcg ggtaaatcca
    18721 cactggcaaa aatgctggcg ggaatgatag aacctaccag cggcgagtta ttgattgacg
    18781 atcatccgct gcattttggc gattattcct tccgtagtca gcgtattcgc atgatttttc
    18841 aggatccttc gacctcgttg aatccccgtc aacgtatctc acaaattctg gattttccac
    18901 tgcgcctgaa caccgacctg gaaccggaac agcgtcgcaa acagattatt gaaacgatgc
    18961 gtatggttgg gctgctaccg gatcacgtca gttactatcc gcatatgttg gcacccggac
    19021 aaaaacagcg tctgggtctg gcgcgcgcgc tgatattgcg cccaaaagtc attattgccg
    19081 atgaagccct cgcttcactg gatatgtcaa tgcgttcgca gttgattaat ctgatgctgg
    19141 agttacagga aaaacagggc atttcgtata tttatgttac ccagcatatc ggaatgatga
    19201 agcacattag cgaccaggtg ctggtgatgc atcagggcga ggttgtcgag cgaggcagca
    19261 ccgcggatgt gctggcatcg ccgctgcatg aactcaccaa acggctgatt gccgggcact
    19321 ttggcgaagc attgacagcg gatgcgtggc gtaaagatcg ctaattttct gaatcctcct
    19381 tccagattat tcacctcatt ctgacaagag attaaatctt gtcagaatga ggtgatggat
    19441 aaaataaaat caaatgcgcg tgatttaaga cgcaatctca ctttgcagga acgaaagctc
    19501 tggcgatatc ttcgcagccg acgttttggt gatttcaaat ttcgccgtca acatccggtg
    19561 gggagctaca ttctcgattt tgcttgctgc tcggcgcgcg tagtcgttga gctggatggt
    19621 gggcagcatg atttggcagt tgcctatgat accaggcgca ctagctggct tgagtcgcag
    19681 ggctggaccg tgctgcgttt ctggaataac gagattgatt gtaatgagga ggcggtgctg
    19741 gagattattc tgcaggaact gaaccgccgg tcaccctcac cctgaaaggg cgagggggcc
    19801 gaccgagccg aatagctgtt gtggtgaaaa catggagaca gtgctggaga atattcggca
    19861 aggtctgaac catcccagcc atcgccataa aagggttagg ggctgtatga gcctgtttgt
    19921 tgctggggta acaatatttg cacaatatgg tcccctcgcc cctctgggga gagggttagg
    19981 gtaaggggaa aagcgccccc cctgccgcag cctgctccgg tcggacctgg taactatagc
    20041 tactcacagc caggttgatt ataataaccg tttatctgtt cgtactgttt actaaaacga
    20101 cgaatcgcct gattttcagg cacaacaagc atcaacaata aggattaaag ctatgggttt
    20161 tctttccggt aagcgcattc tggtaaccgg tgttgccagc aaactgtcca tcgcctacgg
    20221 tatcgctcag gcgatgcacc gcgaaggagc tgaactggca ttcacctacc agaacgacaa
    20281 actgaaaggc cgcgtagaag aatttgccgc tcaattgggt tctgacatcg ttctgcagtg
    20341 cgatgttgca gaagatgcca gcatcgacac catgttcgct gaactgggga aagtttggcc
    20401 gaaatttgac ggtttcgtac actctattgg ttttgcacct ggcgatcagc tggatggtga
    20461 ctatgttaac gccgttaccc gtgaaggctt caaaattgcc cacgacatca gctcctacag
    20521 cttcgttgca atggcaaaag cttgccgctc catgctgaat ccgggttctg ccctgctgac
    20581 cctttcctac cttggcgctg agcgcgctat cccgaactac aacgttatgg gtctggcaaa
    20641 agcgtctctg gaagcgaacg tgcgctatat ggcgaacgcg atgggtccgg aaggtgtgcg
    20701 tgttaacgcc atctctgctg gtccgatccg tactctggcg gcctccggta tcaaagactt
    20761 ccgcaaaatg ctggctcatt gcgaagccgt taccccgatt cgccgtacag ttactattga
    20821 agatgtgggt aactctgcgg cattcctgtg ctccgatctc tctgccggta tctccggtga
    20881 agtagtccac gttgacggcg gtttcagcat cgctgcaatg aacgaactcg aactgaaata
    20941 atcgttctgt tggtaaagat gggcggcgtt ctgccgcccg ttatctctgt tatacctttc
    21001 tgatatttgt tatcgccgat ccgtctttct ccccttcccg ccttgcgtca ggataacgat
    21061 ttcctttacg accaaggagc gcccatggaa caacgccaca tcaccggcaa aagccactgg
    21121 tatcatgaaa cgcaatccag tactacggag tatgacgttc tgcctctggt cccggaagcc
    21181 gcaaaggtca gcgatccctt tctgctcgac gtgatccttg atgaagagac gctggccccc
    21241 ttcctttcat ggctggtccc tgcgcgcgtt cttgcagtgg agttgttccc tgaccagctt
    21301 accgtgaccc gttcacaaac cttcaccgct tatgaacgct tgtcgacggc cctgacggtt
    21361 gctcaggttt gcggcgtcca gcggttatgt aactactatt cggcgcgact tacgccgctc
    21421 cccggacctg attcctccag ggaaagtaat catcggttgg cacaaatcac gcaatatgcc
    21481 cgccaactgg ctagctcgcc ttctattatc gacaaccgat cgcgccagca tctgaatgac
    21541 gtcggtctta ctgtctggga ctgtgtgatc attaaccaaa tcattggttt tattggcttt
    21601 caggcgcgga caattgcgac atttcaggct tatctcgggc atccggtacg ctggttaccc
    21661 gggctggcga tacaaaacta cgccgacgcg tcactgtttg ctgatgaatc aatacgctgg
    21721 cgaagcagct atgaggtgga aaaactacct gaagagcaca caaaaagttc aactgcagaa
    21781 ctttgccaac tggccgaaac actctctctc caccctattt cactttccct tctcgaaaag
    21841 ttgttaaaca gcacacgggt caatgcacag ccggataatc agcttgcggc gttgttatgc
    21901 gcgcgtataa atggcagtcc tgcttgtttt tccacctgta tggattcatc aaatgaatat
    21961 aaaaaaatca gcacccttct gcgcaagggc gaaaatgaaa ttaaccgatg ggctgaccgt
    22021 cattctgttg agcgcgctac cgttcaggcg atacaatggc tgacccgagc acccgatcgc
    22081 tttagcgccg cccagttcag ccctttactc gaacacgaaa aatcatcaac gcagattatt
    22141 aatctgctgg tatggagcgg gctgtgtggc tggataaatc gcttaaaaat cgcgttgggt
    22201 gagacatatt aaccttgccg cgtcagacag attcgcgtaa aactgtcagc cgctctaatg
    22261 gccaccaaaa tagacaatta tgtttcagga caacccgctg ctagcgcagc ttaaacagca
    22321 actgcattcc cagacgccac gcgctgaagg ggtggtaaaa gccacagaaa aaggctttgg
    22381 cttcctggaa gtcgacgcac aaaaaagtta tttcattccg ccgccgcaga tgaaaaaagt
    22441 catgcatggc gaccgaatta tcgcggtgat ccacagtgaa aaagaacgtg aatccgcaga
    22501 gccagaagaa ctggttgaac cgttcctgac tcgtttcgtg ggtaaagttc agggcaaaaa
    22561 tgaccgtctg accatcgttc ctgatcatcc acttttaaaa gacgccattc cttgccgcgc
    22621 agcccgtggc ctgaaccacg agtttaaaga aggcgactgg gcggttgccg aaatgcgccg
    22681 tcatccgctg aaaggcgatc gttctttcta tgctgaactg acacaataca tcacttttgg
    22741 tgacgaccac tttgtaccgt ggtgggttac ccttgcgcgc cataatctgg aaaaagaagc
    22801 accagacggc gtcgctaccg aaatgctcga tgaaggtctg gttcgtgaag atctgacctc
    22861 gctggatttt gtcaccatcg acagtgccag cactgaagat atggatgacg cccttttcgc
    22921 taaggcgctg ccggatgaca aacttcagct gattgtggcg attgccgatc caaccgcgtg
    22981 gattgctgaa ggcagcaagc tggacaaagc cgcgaaaatt cgcgcattca ccaactatct
    23041 gcctggcttc aacatcccta tgctgcctcg cgagctttct gacgatctct gctcactgcg
    23101 cgccaatgaa gtccgcccgg tactggcatg ccgcatgacg ctctccgctg atggcaccat
    23161 tgaagataat atcgaattct ttgccgccac catcgaatcc aaagcgaagc tggtgtatga
    23221 ccaggtttct gactggctgg aaaataccgg tgactggaag cctgaaagtg aagcaattgc
    23281 cgaacaagtc cgtttgctag cgcaaatttg ccaacgccgc ggcgagtggc gtcataacca
    23341 cgcactggtg tttaaagatc gcccggatta ccgctttatt ctcggtgaaa aaggtgaagt
    23401 gctggatatc gtcgccgagc ctcgtcgcat tgccaaccgt atcgtcgaag aagcgatgat
    23461 tgccgctaac atttgtgcgg cccgcgtact gcgcgataag ctcggttttg gcatctataa
    23521 cgtgcatatg ggctttgatc cggcgaatgc cgacgcgctg gcagcgttgc tgaaaacgca
    23581 cggtctgcat gtcgatgccg aagaagtgct cacgctggac ggtttctgca aactgcgtcg
    23641 tgaactggat gcgcaaccaa ctggtttcct cgacagccgc attcgtcgct tccagtcatt
    23701 tgctgaaatt agctctgaac ccggtcctca ctttggcctc ggtctggaag catacgccac
    23761 ctggacttcg ccgatccgta aatatggcga catgatcaac caccgtctgc tgaaagcggt
    23821 tatcaaaggc gaaactgcga cgcgtccgca ggatgaaatc actgtccaaa tggccgaacg
    23881 tcgccgtctc aaccggatgg cagaacgtga tgttggcgac tggttatacg cacgcttcct
    23941 gaaagataaa gccgggaccg gcacccgttt cgcggcggaa attgtcgata tcagccgtgg
    24001 cggcatgcgt gtgcgtttgg ttgataacgg cgctatcgcc tttattccgg cacctttctt
    24061 acacgctgtg cgcgatgaac tggtttgcag ccaggaaaac ggcactgtac aaattaaagg
    24121 tgaaacggtt tacaaagtaa ctgacgttat tgacgtcacc attgccgaag tccgcatgga
    24181 aacccgcagc attattgcgc gcccggtcgc gtaatctcct ttcacggccc attcctcatg
    24241 gatgggccgt ttatttcccc gctctacctt cgttatatcc aggcagattt tttaactgac
    24301 tttcgtttga aaactggcgt ttttccaaaa ctggattaca aataaatata taaaaaaaca
    24361 atgcgtttag ccgccgttaa tttcatgtaa gtcatgttgt tccttttatt taaccgcgga
    24421 ctccgctgtt aaccggagga tatgcatcat gaaaaccgtt agggagtcca caacgttgta
    24481 caactttctc ggatcgcaca atccatactg gcggttgacg gaaagcagcg atgctttgcg
    24541 cttttctacc accaaaacca cagaacctga tcgtacattg cagttatctg ccgaacaggc
    24601 tgctcgcatc agggaaatga cggtcatcac ctccagcctg atgatgagtc tgaccgtcga
    24661 tgaaagcgat ctttctgtgc atctggtagg acgaaaaatc aataaacggg aatgggctgg
    24721 caacgcgtct gcatggcatg acacaccggc ggttgctcgt gatttatcac acgggctttc
    24781 ctttgctgag caggtagttt ctgaagcaca ttccgcaata gtgattctcg acagccgggg
    24841 gaatatccaa cgcttcaatc ggttatgtga agattacaca gggttgaaag aacacgacgt
    24901 cattgggtaa agcgtgttta aactgtttat gagccgtcgt gaagctgcgg catccaggcg
    24961 caataaccgt gtattttttc gaagcggcaa tgcatatgaa gtcgaactgt ggataccaac
    25021 acgtaaaggc cagcggctgt ttctgtttcg caataaattt gtccacagcg gcagtggcaa
    25081 aaacgagatt tttttaatct gttccggtac cgacattacc gaagagcgcc gcactcagga
    25141 gcgactgcgt attctggcaa ataccgacag tatcaccgga ctgccgaatc gtaacgcaat
    25201 gcaggagtta atcgatcacg ctattaatca ggcagataac aataaagttg gggttgtgta
    25261 tcttgatttg gataatttta aaaaggtcaa cgacgcctat gggcatttgt ttggtgacca
    25321 gttattacgc gacgtgtcat tggctatttt aagctgtctc gaacatgacc aggtgttggc
    25381 gcgtccaggt ggcgatgagt ttctggtact ggcatccaat acctcacaaa gcgcgctgga
    25441 agcaatggca tcacgaattt tgacccgctt acgcctcccc tttcgtattg gtttaattga
    25501 agtttatacc agctgttcag taggtatttc gctctctccc gaacatggtt cggacagcgc
    25561 gactattatt cgtcacgccg acacagcaat gtacacagcg aaggaaggcg gacgaggaca
    25621 attttgcgtt tttactccag aaatgaatca gcgggtattt gaatatctct ggctggatac
    25681 caacttgcgt aaagcactgg aaaacgatca gttggttatc gaaaatcacc tagcgtgtcg
    25741 aagtgcgcag tctggaagca ctagtacgtt ggcagtcacc tgaacgtggg ttgattccac
    25801 cgttggactt catttcctac gccgaagagt cagggctaat tgtgccttta ggccgttggg
    25861 tgattctcga tgtcgtacgc caggtggcaa agtggcggga taaaggcata aacctgcgag
    25921 tggcggtaaa tatttctgca cgtcagctcg ccgatcaaac cattttcacc gccctgaaac
    25981 aggttctcca ggaactcaat tttgaatact gccctataga tgttgaactg acagagagtt
    26041 gtctgattga gaatgatgaa ctggcactgt ctgttattca acaatttagc caactaggtg
    26101 cgcaagtgca tctggatgat tttggtaccg gctactcttc actttcgcaa ctggcgcgct
    26161 ttccgatcga tgccatcaaa cttgaccagg tttttgttcg agatattcac aaacaacctg
    26221 tctcgcagtc actggtccgg gcgatcgtcg ctgtggccca ggcattgaat cttcaggtga
    26281 tcgccgaagg tgtagagagt gcaaaggaag atgctttttt aaccaagaac gggatcaatg
    26341 agcggcaagg atttttgttt gccaaaccga tgcccgccgt cgccttcgaa cgctggtata
    26401 aacgctatct gaagcgcgca taagtagatg aatatatata cttagcgcta tatcccgtca
    26461 ttatatatcg tattcctgga tgataattag ccgttgctgt taaacacttc tcttttagcg
    26521 gcaatttttg cattattcat tttccccctt ttgctataag gatgtccttg atgtctgaat
    26581 ttgatgccca gcgcgttgcc gaacgtattg atattgtgtt agatatcctt gtggcaggcg
    26641 attaccactc tgcgatccat aatctcgaga tacttaaagc agaactcttg cgccaggttg
    26701 ctgaatctac gccagacatc ccgaagacac cgtgggagat ttaacaatcg tgccttcagg
    26761 ttttcctatt tgaggatgcc cggcgcgtca gtattttttt atttagtatt ataacgttat
    26821 aagaattaca gcgatgaatt cccgacaaca aactattcta cagatggtca ttgaccaggg
    26881 tcaggttagc gtaaccgatc tggcaaaagc cactggagtt tctgaagtta ccattcgcca
    26941 ggatctcaac accctcgaaa aactgagtta cctccgccgt gcacatggct ttgcagtttc
    27001 gcttgatagt gatgacgtcg aaacccgtat gatgagcaac tatacgctga agcgtgaact
    27061 cgccgagttt gccgcgtcac tggttcaacc gggcgaaacc atctttatcg aaaatggcag
    27121 cagcaatgcc ctgctcgccc ggactctggg ggagcagaag aaaaatgtca ctatcatcac
    27181 ggtcagcagc tacatcgcgc atttgctgaa agacgcgcct tgtgaagtca ttttgctcgg
    27241 tggcgtgtac cagaaaaaaa gcgaaagtat ggttggccct ttgacacgcc agtgcatcca
    27301 gcaggtacat ttcagcaaag catttattgg tattgatggc tggcaacctg aaactggatt
    27361 caccggtcgc gatatgatgc gcaccgatgt ggtcaatgcc gtgctggaaa aagagtgcga
    27421 agcgatagtc ctgactgaca gctcgaaatt tggtgctgta cattcatact ccatcggtcc
    27481 cgttgagcga ttcaatcgcg tgattaccga ttcgaaaata cgcgccagcg atctgatgca
    27541 tcttgagcac agtaaactca ccgttcacgt cgttgacatt taaccatttc ccttctctac
    27601 ggatgatttg cagtttggca aatcatccgc tctaagatga ttcctggttg ataattaaga
    27661 ctatttacct gttattaaca ctctcaagat ataaaattat tatcagcgat ataacaggaa
    27721 gtaattatca cctgcgtgat ataaccctgc gcgcgagcag atttcacgga ataatttcac
    27781 caggcttatt cttagctatt atagttatag agagcttact tccgtgaatc ataaattcag
    27841 gagagagtat tatgtttgta acgagcaaaa aaatgaccgc ggctgttctg gcaattactt
    27901 tggcaatgtc tctgagtgcc tgttctaact ggtctaaacg ggaccgcaac accgcaatcg
    27961 gcgcgggtgc aggggcatta ggcggtgcag tactgaccga tggcagtacg ttgggtacat
    28021 taggtggtgc agctgtcggt ggtgttattg gtcaccaggt cggtaaataa tcgtaaataa
    28081 atgtcgatta tcgactcgca attataatac gcttgattct gttcagtacg aaataactga
    28141 ataatacaca cagtaaaata ttcgaaagcc acggatatat ccgtggcttt ttatgttaac
    28201 cgcctgcgag ttttaccttc atccctttcg cttcaagtag tgattttaat aaatcacgct
    28261 tatcgccctg gatttcaata actccatctt taactgctcc gccgcagccg cattttttct
    28321 tcagttccgc tgcaagtttt gtcagttcgg catcatcgag atcgataccg gtaatcaggc
    28381 aaacgccctt acctttacgt ccactggtct gacgctgaat acgcaccgct ccatcgcctt
    28441 tagggcgtac tggggccgct ttgggctcat caatacgccc ggtttccgtt gagtagacca
    28501 gacggctgtt ggaatcactc atcatgcact ccgctgtaaa gaggcgttga tcgctttcag
    28561 tgtctgcgct ggatctaccg attgcgttac cgggcgacca atcaccatat aatcaacacc
    28621 agccgccaac gcctgttctg gcgtcataat gcggcgctgg tcaccagctt cactcccctg
    28681 cggacgaatg cccggtgtaa ccagtttgaa ctcctgaccg aatacctgtt taaagcgcac
    28741 agcttcctga gcagaacaca ccacaccatc aaggtcacat ttttgcgtca gtgctgccag
    28801 acgttctgca taatctgcag gtgacagtgt cacgccaaga tcggccaggt cgctggcttc
    28861 catgctggtc aacactgtca cagcaatcaa aagcggtgca tctttgccaa acggaaccag
    28921 tgcctcacgc gctgcggtca tcatacgcgc cccaccggag gcatgaacat tcaccatcca
    28981 cacgcctaag tcagctgcag cagcgacagc gtgcgctgca gtgttgggga tatcgtggaa
    29041 tttcaggtca agaaagatat caaaaccacg ctgttgaagt tcgcgcacaa actgtggccc
    29101 aaacaatgta aacatctctt taccgacctt cagacgacaa tcgcgtgggt cgatcttgtc
    29161 gacaaaggac agcgcgtcat cacaattatg ataatcaagg gcaacaacca caggagaatt
    29221 cgtaacggcg cgggaagaag atgaagcagt taacgtcatg accagacctt cttgatgatg
    29281 ggcaccggga gtggcgcgaa acaggtaaac ggcgagcatt ctacccgtga aaataaaaaa
    29341 ttaacagttg cgatttttcc cctagcaggc tttttctgct ccgtcgttgc cggagcacaa
    29401 tgggacatca ataactagta tgttgtaact aaagtcggat tttttaaaaa attacaggcc
    29461 atcaagaccg cgaatcggtt taatagttga ccaggcacga caagacggac aatgccagta
    29521 gagggtgtat gcggtaaaac cacatttctg gcagctataa cgaggcttac tacgcacctt
    29581 ctcgccaacc atgtcacgca gcaccatcag actctctttg gcgcgccctt cttccgcttc
    29641 atttaagtgg taatccatta gcttatggaa tacacgcatg gtcggatgac gctgaagctg
    29701 gcgtgtaata tagacctgtg cagcctcact accgtcgcgc gcttcgatga tatcagcaag
    29761 catcaattca gcatcggcac cggtgttctc ttccaccgcg cgttgcagga attctgccca
    29821 ttcggcagtt ttacccaact gctgatagca ggtttgcaac atttccagcg tttcgctgac
    29881 cagttctctg tcctgggata tgacgcgttg caaactttcg acggctttgg cgtattctcc
    29941 tttcgccata aacacgcgtc ccatcatgat ggatacgcgg gcgctgtttt tatctgccgc
    30001 cgcccctttt tttagcaagg tcatggcacg atcgagatcg tcgctggcca tatgctgcag
    30061 ggctaactca cagtagaaat gggcaatttc gacgcgctgt ttatctttac ccagcttcac
    30121 caggcgttcg gcaacatcaa ttgctttctg ccactcactg gtagcctggt agatttgtag
    30181 caactgttgc agcgcgccaa tgcggaagtc agtttcatcg gtcagctgat tgaacatgtc
    30241 ttccgcgcgg tcgtataacc cggcggccat gtaatcacgc cccagttgtt gaatcgccaa
    30301 cagacgctgt tcataggtca gcgaggcgct ttccattagg gtctgatgga tgcgaatagc
    30361 gcgatcaact tcgccacgcg aacggaacag gtttccgagc gtaaggtggg cttcaacggt
    30421 gcctgtatcc tctttaagca tatcgagaaa caggtctacc gctttatcct gttgattact
    30481 aagcaggaag ttaacccccg ctacgtaatc acgcgacaag cggttggctt catcttgctt
    30541 gttttgttgc gcacttctgc ggcccatata ccagccatag gcagcggcta caggcaaaag
    30601 cagaaacaac aactccagca tagaaagtta ttccttcgcc gctgacgagt gcggcactac
    30661 agccacgtca gtcgcgggtg aaagctggtt ttccagtcgc tttattttac gttcagcgcg
    30721 cgccagggaa acacgaactc gcagccagaa caggccacaa atcaaccaac cgatagcaaa
    30781 ccccgcagca aacaataccg ccagcaatgt ggaaatacgg tactcccctt gcgctaacag
    30841 ataattaaac gtcacctgtt gatcgttctg cgcacccaac gtgaccgaaa tcacgaagat
    30901 cgctaacacc agtaagaaaa tgagtaaata tttcacatta cttcccgtta tgtggttaga
    30961 gcgaataaat tatggtcaac ttaccgcaat agccctttaa agtaccattt tcgcgccgtc
    31021 tgcgaaatgg catccacgcg cattgttgat gatttaaggc ctaaattcgg aaaatcaacc
    31081 agcgttaact ttcttgttct cgttgtgcaa tttcgcgatt ttcttccgca ggtggtgtta
    31141 atggcccaca aattcgttgc gcaagccacg ttgccaccgc caccagcgcc cacgaaatca
    31201 acgtagctac caccagatcg cgtggccaat gcatcccgag cagcaggcgg cttcccatga
    31261 ctcccgttgc ccagaccaac aagatagcaa tggttaacgt tcgccgacgc ggccacagca
    31321 aaccaacggc cagcagtgcc caactggcag caaacatcgt gtgaccggaa ggaaaggcaa
    31381 accccgtctc tttctgccag tgtgaacgca aatattgtgg aatatttttc tcttcagcca
    31441 actgttcttt tactagattt ccgcgttctg ctcgctttaa agtgtagaac tcatcaaccg
    31501 gaatatgatg tgttttttcc aaccagataa caaaaggtcg tggttcctgt actttgtctt
    31561 tgatccagga tttaacgcct tgtccaacaa ggattgccgc cgccagaatg gcaaataata
    31621 caaaggcagc cttaatgcga aaacgcagac accagagaaa ccagccgaac aaaatcaaat
    31681 gtgtaatgac gccccagggc tgggtgacag tttcagtaac ccaaaaagcc gcttttagta
    31741 gccaactttg ttctccaggt tgccaacgcc agccagaaat ccatacggct actggcatga
    31801 caagcaatag tgcagctccc actgcggtac gtcgggcaat cgaacgcatg gcctctcctt
    31861 tttgataagt cccacaatca taactgaaaa tgccagttcc aagaaaaatt gacagatttg
    31921 tgccattccg tgaacgatcg acgcgtcgtg attaggtgaa ccccttctcg ttatggcaaa
    31981 ataagccaat acagaaccag cattatctgg agaatttcat gcagcttaaa cgtgtggcag
    32041 aagccaaact gccaacccca tggggcgatt tcctgatggt gggatttgaa gaactggcaa
    32101 ccggacacga tcatgttgcg ctagtctatg gcgatatttc cgggcatacc ccggtacttg
    32161 cgcgcgtcca ttccgaatgt ctgaccggtg acgccctgtt cagcttgcgc tgcgattgtg
    32221 gcttccagct cgaagcggca ttgacgcaaa ttgccgagga aggccgtggt attttgctgt
    32281 atcaccgtca ggaaggtcgt aacattggtc tgctgaataa aatccgcgct tacgcactgc
    32341 aggatcaagg ttacgatacc gtagaggcta accaccagtt aggcttcgcc gctgatgagc
    32401 gcgacttcac tctttgcgct gatatgttca aactccttgg cgtcaatgaa gtccgcttgt
    32461 taaccaataa cccgaaaaaa gtcgaaattc tgaccgaagc agggattaat attgttgaac
    32521 gcgtaccatt gattgtaggt cgtaacccca ataacgaaca ttatctcgat accaaagccg
    32581 agaaaatggg ccatttgctg aacaaataac cctcttgcat cgtgtaattc atttgcttgc
    32641 cggaagcaaa ataaccggca agcaaatact tgttacttca acatattacg aatgacataa
    32701 tgcaaaatgc cgtcgttctg gtagtaggtc aactccgtcg cggtgtcgat acgacaacgg
    32761 cagggtacga cttcctggct accatccgcg cgcgtaagcg tcaccggaac cgtcgcgcca
    32821 ggttgtagat tttgcagatc gccaatatca atcttctctt ccccggttag ccctaacgtt
    32881 ttacgcgtca cgccttgcgg aaattccagc ggcaggatgc ccatgccaat taaattcgaa
    32941 cggtgaattc gttcaaacga ttcggcaatc accacacgaa ttccaagcag acgaggtcct
    33001 tttgccgccc agtcacggct tgagcctgat ccatactctt tcccggcaat caccgccagc
    33061 ggcgtttgct cctgcttata gcgcatcgca gcatcataaa tagagactac gtcgctgtca
    33121 ggtaaatgcc gcgtcatccc cccttcaacg ccaggcacca tttcattacg gatgcgaata
    33181 ttggcgaagg tgccgcgcat catcacttca tggttaccac gccgcgaacc gtaggagtta
    33241 aagtcttttc gctcaacacc ccgaccttgt agatatcgac ccgctgggct gtcgggctta
    33301 atactgcccg ccggagagat atggtcagtg gtgactgaat cccccagcat tgcgaggatc
    33361 cgcgcaccgt gaatatcttc cactggtgct ggtgttgcct gcatttcatc aaagaaaggc
    33421 gataagcgaa tataggttga gtcctcctgc caaccgtagg tatcggatcg tgtgacgtta
    33481 atttccttcc actctgctgt gccttcaaaa acttctgcgt actctttgcg gaacatttct
    33541 gtggagactt gttctaccgc acgggcaatt tcttgtgccg atggccagat atctttcaga
    33601 taaaccggct cgcctttgcg atcatggccg ataggctcag aagccaggtt gatattcata
    33661 tttcccgcca gcgcataggc aaccaccagc ggtggcgagg ccagccagtt agttttaacc
    33721 agcggatgga tacggccttc aaagttacgg ttgccggaca gcaccgcacc gacggttaaa
    33781 tcgccttttt tgattgccgt ttcgatagga tcgggcagcg gcccagagtt accaatacag
    33841 gtggtacaac cgtatcccac aaggttaaac cccagttcgt cgagatacgg tgtcagtttc
    33901 gcttttgcca gataatcaga aacgactttc gaacccggtg ccaacgacgc tttgacccac
    33961 ggttgccgct tgaggccaag agttacggct tttttcgcca gcaagcctgc ggccatcagc
    34021 acacttgggt tagaggtgtt ggtgcacgag gttatcgcag caatgaccac agcgccatca
    34081 ggtaactgat actgatgtcc gttcataaca taatcgactg gcaggcgatc tttatgcgtg
    34141 gcattcactt tcagttcgtt actggcggca aatgcttttg gtacatcggg cagtgcaacg
    34201 cgatcctgtg ggcgttttgg ccctgccagg ctcgcttcaa cgtcattcat atccagttct
    34261 aacatactgg taaaaatcgg ttcatcgccc gggttacgcc acatgccctg cgctttggca
    34321 tatttttcga ccaactcgac ctgatcttcg ctgcgcccgc ttaaacgcat gtaatcgagg
    34381 gttactgcat cgattgggaa gaagccacag gtggcaccat attctggcga catattggca
    34441 atggtggcgc gatccgccaa cggtagtgaa tccagaccat caccataaaa ttcgacgaat
    34501 ttccccacca cgccatgttt gcgcagcatt tgggtaacag tgagaaccag gtctgtggcg
    34561 gtaatacctt cacgtaattt tccggtaagt ttaaagccca ctacgtccgg gataaacatg
    34621 gaaaccggct ggcctaacat ggcggcttct gcttcgatcc cacccacgcc ccaccccagc
    34681 acgccaaggc cgttgatcat agtggtgtgc gagtcagtac caacgagtgt atccggataa
    34741 gcaatccatt caccgtcctg caattcgctc cacactgctt tgccgagata ttcgaggtta
    34801 acctgatggc aaatgcctgt gcctggcggc acgacgctaa accgactgaa cgcttgcttt
    34861 ccccatttca ggaacacata acgttcgtgg ttgcgctcca tttccaggcg tacgttttct
    34921 tcaaatgcct catcatcacc aaaacgatcg acggtcaccg agtggtcaat gaccaggtcg
    34981 accggtgaga gcgggttaac ctttgcagta tcgccaccga ggcgtttaac cgcttcgcgc
    35041 attgccgcca gatcaacaac ggcaggtacg ccggtaaagt cctgcatcag cacccttgcc
    35101 gggcggtagg caatttcacg gtcagcatgg gcatttttca gccatcccgc cagtgcgtgg
    35161 atatcctctt cggtaactga gttaccatcc tgccagcgca gcaggttttc gagcaaaact
    35221 ttgagtgact tgggtagacg ggtgatatcg cccagtgatt tagcagcaag cggcaggctg
    35281 tagtagtggt aagttttatc tttggcctgc aacgtgtcct tactggcttc tcgtagggtt
    35341 gacgacatag ctcctcctta atgacagggt tgcgaagccc tgattctctt cagggtgaat
    35401 attaaagata acacaaacat cgcgtaacga tttgataaca acccaaattg ataaaagagg
    35461 agaaaccttg tggcaaagca aatgacaacc ccgccgcagc ggggtcaagg atcagtgagt
    35521 aatgatccag aggagttcgg cccagaagag catcgacaga gaaaacgcgc ccatccagga
    35581 ccaatatttc atatttgtgt tgatcatagg ctacaccctt aacggttatt attttgtgtt
    35641 gagataactc aagctgttat tgctgcggtg tattaaaagc cctttaattc attaaaaatg
    35701 ggtaataaga atgatgataa ttattgtttc ggctgatcat cttcagcaaa cagttctata
    35761 aaggcttgct gctgcgatgt tagttgccag tcgtctggaa taacgacgga acattcccgt
    35821 ttcgtttctg gttcattgtc actatgctgg cataatggtg acgactccag ttgctcctga
    35881 ccgaccatag ttatccccac actttccaaa tcagcagtgc gacaaccacc cagaaaagtg
    35941 cagaaccaag aaaaaccgcc agccaggctt tacgttttaa ttcaggatca cggcggggtt
    36001 cctgatttcc agaaggcatt gctaacctca tataatcgcc atcacttatc agcaagacgt
    36061 tgaatgattg gtgtaattaa tcatacgatg agacaaatcc taaagaaact ttagtcaaaa
    36121 atccagcgaa tttacgaatc gtctccagtg aataattcaa tcttttgacc tgaaatagat
    36181 atttgaaaac tggaatttgc gaggcgggta attagacact actttacagg taatttaccc
    36241 tataaatatg agggataaaa atattgtgca cggtatagaa ataagtaaca aaagagaata
    36301 tattccggca ccttcgctac ataaaaggtg ccgaaaataa cgcaagaaat tatttttccg
    36361 gcagttttat atctttaaac atgacctcaa tttcttcatt ggagcgcaat gcgacagccg
    36421 catcaacgac atcacgcgtt aaatgcggtg caaaacgctg aatgaaatca tacatataac
    36481 tgcgcaaaaa agtactacgg cgaaaaccaa ttttggttgt actgtggctg aagatatcgt
    36541 gagcatcaac gcgcacaagg tcgggatcgg cgaccggatc caccgccatg ctggcaatga
    36601 cccctacccc cagccctaac cggacgtaag ttttaatgac gtcagcatcc gttgccgtga
    36661 aaacgatacg cggcgttaac cctgcgcggt taaaggcagt atccagttct gaacgtccgg
    36721 taaagccgaa ggtatatgtc accaacggat attgcgccag ttcttcaatg gtaatggctt
    36781 ttttgcctgc cagcgggtga tccggagtga ctacaatagc ccgattccag tggtagcacg
    36841 gtaacatcac taaatcttca tacagatgca gcgcttctgt ggcgatagcg aaatcggcat
    36901 tgcctttaga gacggcatca gcaatttgtg tcggcgggcc ctggtgcata tgcaaagaaa
    36961 cgcggggata acgctcaata aagcctttga taacgtttgg taacgcgtag cgtgcctggg
    37021 tatgcgtggt ggcgatatac agcgagcctt tatccggcca ggtgtgctca ccggcaaccg
    37081 attttatggc atcgactttc gacaggactt cgcgagcgat acgaattatt tcttgtcctg
    37141 ctggcgttac ctgcgtcagg tgcttgccgc ttcgggaaaa aatttgaatg cctagctcgt
    37201 cttccagcat tctgacttgt ttactgatcc cgggttgtga tgtgtaaagt ccttccgctg
    37261 ttgatgagac attcaggtta tgattgacca cctcaacaat atagcgaagt tgttgtaatt
    37321 tcatgttaaa ccatccactt agacgccatc agaacatcgt tttttcgtca cacctggcgg
    37381 gtgactaata cgatttaata ataaaaaagg tgctaactat aaccactata tcatttatag
    37441 ccttagtgta taggtgtgaa gaaaaaataa tatgcaataa aaaagggccg ctttcgcgac
    37501 cctttgttta taaaaacctg acagaattaa aggttatttt tttccttcaa cccatttgcc
    37561 atcaacataa aatgctgacc agccagtcgc ctttccgtct ttttccgaag agacatattg
    37621 ctgtttggtt ttacggctaa agcgaaccat ggtcttatta ccttccggat cctgctgtgg
    37681 cgcatcggcc agataacgca gtttttccgg cagacggtcg cggaagcgat aaagctcttc
    37741 caccagtggc gcacgcgttt cacgcgattt cgggaaagtg ttggcagcca ggaacacacc
    37801 ggcagcaccg tcacgcagca cgaaataagc atctgatttt tcgcacggca gctcaggtaa
    37861 tggcaccgga tcttctttcg gtggtgccac ttcaccgtta cgtaaaatct tacgtgtgtt
    37921 tttacactct tcgttggtgc aggccatgta tttaccgaaa cgccccattt tcaggtgcat
    37981 ttcagagcca catttttcac actcaacgat cgggccgtca taacctttaa tgcggaattc
    38041 gccctcttcg atctcgtaac cgtcgcaggt tgggttatta ccacagacat gcaacttacg
    38101 tttcggatcg atgagatagc tgtccatcgc cgtgccgcat ttcgggcaac gacgttttgc
    38161 gcgcagcgcg ttggtttcag catcttcgcc ttccagcacg ttcagcactt cgttttccgg
    38221 caccaggtta atggtggttt tgcaacgctc tttcggcggc agcgcatagc cagaacagcc
    38281 aaggaatacc ccggtactcg ctgtgcgaat ccccattttg cgaccacaag tcgggcagtc
    38341 aatgctggtc agaaccatct ggttcgggcg cataccaccc tcttccggat ctttttcagc
    38401 tttatctaac tgctgggtga aatccgagaa gaagtgatcc agtacagctt tccactctgc
    38461 ttcgtgattt gccacctggt cgaggctgtt ttccatctgc gcggtaaagt cgtagttcat
    38521 taactcgcgg aaattttctt caaggcgatc ggtgacgatt tcgcccattt tttccgcata
    38581 gaaacgacga ttttctactc gcacatagcc acgatcctga atggtcgaaa tgatcgacgc
    38641 ataggtagac ggacgaccga taccacgttt ttccagttct ttaaccagcg atgcttcact
    38701 gaaacgggct ggcggcttgg taaagtgctg ggctggtgtt agttcaacga gcgtcagagc
    38761 atcgccttta tcgactgccg gtaagatacg atcttcatcg cctttacgca gtgcaggcat
    38821 cacttttgtc cagccatcaa agcgcaaaat acgaccgcgt gctttcaggc ggaaatcgcc
    38881 cgcaccaacg gtcagcgtcg tggagtcata tttcgctggg gtcatctggc aggcaacgaa
    38941 ctgacgccag attaactggt acagtttctg cgcatctgct tccatatcct tcagcgattc
    39001 cgccatcaca ttgacgtcag aaggacgaat cgcttcgtgc gcttcctgtg agttttcttt
    39061 gctggcgtac tgattcggac tttccggcag atatttctta ccaaaattat cgctgatata
    39121 accgcgaacc atatttaccg cgtcctgact caggttagtg gagtcggtac gcatgtaagt
    39181 gatatagcct gcttcataca aacgctgcgc catcatcatg gtttttttca cgccaaaacc
    39241 aagacgggtg ctggcagctt gttgcagcgt agaggtaata aaaggagcgc caggtttact
    39301 ggttgtcggt ttgtcttcac gttccagcac gctgtagcgc gctttttcca gcagacttac
    39361 cgcagcctga gtttgttctt tgttgaccgg acggaacggt ttgtcattct gatgagtcac
    39421 ctgcaactcc aacgcttcac cagatggcgt agtcgtgctg gcatcgactt cccagaactc
    39481 ttccggtacg aacgctttaa tttcacgctc acgctctacc accaggcgga ccgccaccga
    39541 ctgcacacga ccggcagaca gaccacgagc gatctttttc cacagcagcg gcgaaaccat
    39601 ataccccacc acgcggtcca taaagcgacg cgcctgctgg gcattaacac gatcaatatt
    39661 cagctcaccc ggtttgttaa atgcctggcg gatcgcgttt ttagtaattt cgttaaacac
    39721 cactcggcta tagcgcgcat catcaccacc aatcacttcc cgcaggtgcc atgcaatggc
    39781 ttccccttcg cggtcaaggt cggttgcgag atagatgtgg tcggcttttt cagccagttg
    39841 tttcagttca gagacgacct tctctttacc aggcaacact tcatagtgcg cctcccaatt
    39901 gtgccacggg tcaaccccca tacggttgac gagagcgcca cgttcatcct ttttaggctt
    39961 tttagccgtc ttggtggagg tagagtcggc actctttttg gcagctgagc cactggtcgg
    40021 caaatcgcgg atgtgaccga cgctggattt caccacgtag tcacaaccca gatacttgtt
    40081 gatcgttttg gcttttgccg gggactcaac gatgacaaga gctttaccca tattcacctt
    40141 acctaatttg attcttccag gaatgcgtcg cacgttgata aaccttccac tggcaacgag
    40201 ttaccgatat ggactcgctc tctgcggata tcaacccctt gtcgtgactc tgttcacgaa
    40261 aattcaggtg actgagttaa caggtcttgg cctacaggcg ctatagcacg acggattttt
    40321 ggtcgaatgt caagcaaatc tattgccaga ttgacgaaag cgtcacactg tacctgataa
    40381 aatccgatac gcaactttat tagcatgcaa aatccaaagt tgccagtaag gcgctcttgc
    40441 caccagcgtg gtatatttgt ctgcaagcca tgcctggcgt agactaatgt ccttgtttaa
    40501 ggagtaaata atgaacaaag agacacaacc catcgaccgc gaaacgctgt tgaaagaggc
    40561 caacaaaatc attcgtgagc atgaggatac gctcgcaggg attgaagcca ccggcgtcac
    40621 gcagcgtaat ggcgtgctgg tatttaccgg cgactacttt ttagatgaac aaggattgcc
    40681 aacagccaaa agcaccgcgg tgtttaatat gttcaaacac ctggcccatg tgctttcaga
    40741 aaaatatcat ctggttgatt aaagcaaaac gcgaggtctt agcctcgcgt ttgtctttta
    40801 catcaatggc ttttgacccc gctgccacca gcgtagcaac aatcgatcgg cgctctctgc
    40861 cgcgctgccg gtgaatcggt caatgagtcg tttacgctgc atatagcgta cattaaccac
    40921 ttcacggcct tccatcaggc taagaataac ttcatcactg gtgttgattt catcaaccag
    40981 gcctttctct accgcctgtt gtccgtacca gtgttcaccc gttgccacct gttcaatatc
    41041 cagagacgga cgcatacgct tcacaaaatc tttaaataac tgatgcgttt cgttcagctc
    41101 ttcgcggaat ttctcccgcc cttcttcggt attttcaccc agcaaggtca gcgtgcgctt
    41161 atactgcccg gcggtgtgca gttcgatatc aatatctttg cttttcagga agcggttaaa
    41221 gttgggcatt tgcgccacca ccccaatgga acccacaata gcaaacggtg cggaaacaat
    41281 tttgtccgcc acacaggcca tcatgtaacc gccgctggca gcgactttgt ctaccgtaac
    41341 agttaaagga atgtttttat cacgcagacg ctgcagctgc gaagccgcca acccgtaacc
    41401 atgcaccatg ccaccagggc tttccagacg gagcacaacc tgatcctgcg gtttgaatgc
    41461 tgcgagtacc gccgttatct cttcacgtag cgagttcact tcatgggcgt ccatgctgcc
    41521 tttaaaatcc agcacccaga cgcgaggttt actgtcagtt gccacctcgc ccagtttggc
    41581 tttcgctttt gctgctttcg cttcttgctt gtgctttttc ttctgcgctt tgtgccactg
    41641 tttttgctga tgtgagtcca tcagcgccgc ggccagttct tctttcatct ccttatactg
    41701 ttcgctgaga ttgttgaccc gtaactcgcc acgctggcgt ttattacgtt gagcaacatt
    41761 gacaataatg gcggcaatcg ccgcaatcgc tagcacaacg gtaacgattt tagccaaaaa
    41821 caaaccatat tcagacaaca attccacgag tccccacctt ggttaaacaa cgctttggct
    41881 cacagtttac aacaggcatg ttaagccgtc tcgcagagat gttattgcag cgaaagtatg
    41941 acaaagtatc cattttgcgg tgttatcgcc ccaggctgat tgaaatgccc ttctgtttca
    42001 ggcataaagc cccaaagtca taaagtacac tggcagcgcg acagacaacg tcgttgagga
    42061 gtagccatgc attaccagcc aaaacaagat ttactcaatg atcgcattat cctggtgacg
    42121 ggagccagcg atgggattgg tcgtgaagcc gcgatgacgt atgcacgcta tggtgcgaca
    42181 gtgattctgt tgggccgtaa tgaagaaaaa ttacgtcagg tagccagcca cataaacgaa
    42241 gaaactgggc gtcagccaca gtggtttatt ctcgatttgc tgacctgcac gtccgaaaat
    42301 tgccaacaac tggcacagcg cattgtcgtt aattatccgc gtctggatgg tgttttgcat
    42361 aatgccggat tgctcggcga tgtttgccca atgagcgaac aaaatccgca ggtctggcag
    42421 gacgtcatgc agatcaacgt taatgccacc tttatgctca cccaggcact gcttccttta
    42481 ttactcaaat cggacgccgg ttcactggtc tttacttcat caagcgttgg acgtcaggga
    42541 cgagccaact ggggtgcata tgcagcgtcg aaatttgcca ccgaagggat gatgcaggta
    42601 ctggccgatg aatatcagca gcgcctgcgc gtcaactgca ttaacccagg cggtacgcgc
    42661 accgcaatgc gcgcaagcgc cttcccgacc gaagatccac agaaacttaa aacacccgct
    42721 gatatcatgc cgctctacct ctggctgatg ggcgatgaca gccgccgtaa aaccggcatg
    42781 acctttgacg cccaaccggg ccgtaaacca ggaatttccc aatgagtgat gaacgctacc
    42841 aacagcgtca gcagcgagtg aaagaaaaag tagatgctcg tgtggcccag gcccaggatg
    42901 aacgcggtat tatcatcgtc tttaccggca atggaaaagg caaaaccacc gcggcatttg
    42961 gtacggcaac acgcgcagtt ggtcacggaa aaaaagtggg cgtcgtgcag tttattaaag
    43021 gcacctggcc taatggcgaa cgcaatctgc tggagccaca tggcgttgag tttcaggtga
    43081 tggcaacggg ctttacctgg gatacacaaa accgcgagtc tgataccgcc gcctgccgcg
    43141 aagtctggca acatgcaaag cggatgcttg ctgattcctc actggatatg gttttgcttg
    43201 atgaactgac gtatatggtg gcgtatgact atttgccact ggaagaagtg gtgcaggcgt
    43261 taaatgaacg tccacatcaa cagacggtga ttatcacggg tcgaggctgt catcgggata
    43321 ttcttgaact ggcggatacg gtaagtgagc tgcgccccat cagacatgcg tttgatgccg
    43381 gtgttaaggc gcaaataggg attgattatt aatctgtaaa aatgccggat atgacgtccg
    43441 gcatactttg attaaccgtt attacgcccg ccagaacgac gactgccgct cacctgactg
    43501 tgacgtttca ccgcacgacg aatctgatta gccttcatgc gacgacggtc tttttctaca
    43561 gcgactttag agctggtttc tggcggaagc tccaccagtt cgcgcagata gttagtctgg
    43621 gcgaggtcca gctccgtcca gccaccgcgt ggcagacctt ttggcagtgg gatatcaccg
    43681 taacgaacac ggatcaggcg gctcacctgc acaccaaccg cttcccacag acgacgaact
    43741 tcacggttac ggccttcggt cagagtcacg ttgtaccact ggttgatccc ttcgccgccg
    43801 ctgaacttga tggttttaaa agctgccgga ccatcttcca gttgcacgcc acgactcaaa
    43861 tcacgcagtt tcgcgtcgtc aacctgacca aatacacgca cggcgtattc acgttcaact
    43921 tcacggcttg gatgcattaa acggtttgcc agttcaccat cggtggtgaa cagcagcaga
    43981 ccacaggtgt taacgtccag acgacctacg gcaatccagc gtgcgccacg cagctttggc
    44041 agacggtcaa acaccgtcgg acgcccttcc ggatcgttac gggtacacaa ctcaccttcc
    44101 ggtttgtaat aggccagaac gcgacaaatt tgttcagcgg actcacgtac cgaaatcaga
    44161 tgaccatcga tacgaatttt cagcccaggg gtaacttcaa cgcgatcgcc gagtttagca
    44221 attttgccat caacactcac acgaccggct tcaataatag attcgatttc acggcgggag
    44281 ccgtggccag cgcgggccag cactttctgt aatttttcgc tcatagagct tccttttggt
    44341 gtcgccttca caggcgtcga acaggagaat caatggcgcc gtatagcgcc attttaaggc
    44401 cgcgtagtat acagagttac gcacttataa gaaaggcttc acatcaccta cgccttcacg
    44461 caccacgacc ggcgtatcat cggtaagatc aataaccgtt gttggtttct gcccgagata
    44521 gccgccgtga ataatcaaat ctacctgttt ttccagacgg tctttaattt cttcgggatc
    44581 ggattcggta aattcgctgc ctggcagcat tagcgaagtg gaaagcatcg gttcgcccag
    44641 tgcttcaagc aacgcctgcg caatcgggtt agaaggcaca cgcataccga tggttttgcg
    44701 tttctcctgc aacagacggc gtggcacttc cttcgtcccc ttcaggatga aggtatagtt
    44761 gcccggcgtg ttgtttttca tcaaacgaaa cgcaacgtta tccacgaatg aataggtcga
    44821 gagttcagaa agatcgcgac acatcagggt aaagttgtga ccgtccggca gctggcgaat
    44881 acgacaaata cgctccatgg cgtttttatc ttcaatttta cagccgagcg catagccgga
    44941 atcagttgga tagacaatca ccccgccttt acgcacgatc tccaccgcct ggttgatcag
    45001 acgttgctgc gggttatcag gatgaatata aaaaaactgg ctcataattc cctctctgtg
    45061 gtgttctgcg gctgttccca tagctgccag acgccttcaa cgcctgcggg cagccagagt
    45121 ttacgaccca gttcgatcca cgggcatggc tgatgaaaat cagatccttg tgatgcccat
    45181 aaatgatgct gacgcgcaag tgtggccagc tgggtgcgtt cattgggcga ttgctggcac
    45241 tgcgcgactt ccatcgcgtc gccgtggtgt tcggcaaaat gcgcaaccaa tcttttcagc
    45301 catttagcac taagattgta ccgcccagga tgagctagca ccgccttacc gccagaatga
    45361 tgaatgacat caatagcttg ttctattgta caccactgtg gcggaacgta tccggttttc
    45421 ccgcgcgcca gatacttttt aaagacatct gccattgaac tggctttgcc gcactccaca
    45481 aggaaacgcg caaaatgacc gcgcgtcact gcgccgccct gcgccagtcg ttgtgcgcct
    45541 tccagcgccc caggtatttg cgctttttca agtctttcgg caatttgctg cgcccgctga
    45601 ttgcgccgtt ctgtctgctg cgcgaggaac tcacacatta gcggatgagt aatatcaata
    45661 ttcagcccga caatatgaat ttcatgattt tcccagaccg tggaaatttc cacgccggga
    45721 ataagattca gcgccagtcc cgaacgtgaa atttcctctc ttgctggcgc gattgcagct
    45781 gtggtgtcat ggtcggtgat cgccagggtg ccgacgcgca tctcgactgc acggtgcacc
    45841 aatgcttctg gcgtcaggca gccatcggaa gctgtggtat ggctgtgcag gtcgtaaatc
    45901 actgcataat tcgtgtcgct caaggcgcac tcccgttctg gataatgttt tttgcgccga
    45961 catcataacg ggtctggcaa atattctgaa atgagctgtt gacaattaat catcgaacca
    46021 gttaactagt acgcaagttc acgtaaaaag ggtatcgaca atgaaagcaa ttttcgtact
    46081 gaaaggttgg tggcgcactt cctgaaacgg gcagtgtatt caccatgcgt aaagcaatca
    46141 gatacccagc ccgcctaatg agcgggcttt tttttgaaca aaattagaga ataacaatgc
    46201 aaacacaaaa accgactctc gaactgctaa cctgcgaagg cgcttatcgc gacaacccga
    46261 ctgcgctttt tcaccagttg tgtgggaatc gtccggcaac gctgctgctg gaatccgcag
    46321 atatcgacag caaagatgat ttaaaaagcc tgctgctgat agacagtgcg ctgcgcatta
    46381 cagcattagg tgacaccgtc acaattcagg cactttccgg caatggcgaa gccctgctgg
    46441 cactactgga taacgccctg cctgcgggtg tggaaagtga acaatcacca aactgccgtg
    46501 tgctgcgctt cccccctgtc agtccactgc tggatgaaga cgcccgctta tgctcccttt
    46561 cggtttttga cgctttccgt ttattgcaga atctgttgaa tgtaccgaag gaagaacgag
    46621 aagcaatgtt cttcggcggc ctgttctctt atgaccttgt ggcgggattt gaagatttac
    46681 cgcaactgtc agcggaaaat aactgccctg atttctgttt ttatctcgct gaaacgctga
    46741 tggtgattga ccatcagaaa aaaagcaccc gtattcaggc cagcctgttt gctccgaatg
    46801 aagaagaaaa acaacgtctc actgctcgcc tgaacgaact acgtcagcaa ctgaccgaag
    46861 ccgcgccgcc gctgccagtg gtttccgtgc cgcatatgcg ttgtgaatgt aatcagagcg
    46921 atgaagagtt cggtggcgta gtgcgtttgt tgcaaaaagc gattcgcgct ggagaaattt
    46981 tccaggtggt gccatctcgc cgtttctctc tgccctgccc gtcaccgctg gcggcctatt
    47041 acgtgctgaa aaagagtaat cccagcccgt acatgttttt tatgcaggat aatgatttca
    47101 ccctatttgg cgcgtcgccg gaaagctcgc tcaagtatga tgccaccagc cgccagattg
    47161 agatctaccc gattgccgga acacgcccac gcggtcgtcg cgccgatggt tcactggaca
    47221 gagatctcga cagccgtatt gaactggaaa tgcgtactga ccataaagag ctgtctgaac
    47281 atctgatgct ggttgatctc gcccgtaatg atctggcacg catttgcacc cctggcagcc
    47341 gctacatcgc cgatctcacc aaagttgacc gttattccta tgtgatgcac ctcgtctctc
    47401 gcgtagtcgg cgaactgcgt cacgatcttg acgccctgca cgcttatcgc gcctgtatga
    47461 atatggggac gttaagcggt gcgccgaaag tacgcgctat gcagttaatt gccgaggcgg
    47521 aaggtcgtcg ccgcggcagc tacggcggcg cggtaggtta tttcaccgcg catggcgatc
    47581 tcgacacctg cattgtgatc cgctcggcgc tggtggaaaa cggtatcgcc accgtgcaag
    47641 cgggtgctgg tgtagtcctt gattctgttc cgcagtcgga agccgacgaa acccgtaaca
    47701 aagcccgcgc tgtactgcgc gctattgcca ccgcgcatca tgcacaggag actttctgat
    47761 ggctgacatt ctgctgctcg ataatatcga ctcttttacg tacaacctgg cagatcagtt
    47821 gcgcagcaat ggtcataacg tggtgattta ccgcaaccat attccggcgc agaccttaat
    47881 tgaacgcctg gcgacgatga gcaatccggt actgatgctt tctcctggcc ccggtgtgcc
    47941 gagcgaagcc ggttgtatgc cggaactcct cacccgcttg cgtggcaagc tgcccattat
    48001 tggcatttgc ctcggacatc aggcaattgt cgaagcttac gggggctatg tcggtcaggc
    48061 gggcgaaatt cttcacggta aagcgtcgag cattgaacat gacggtcagg cgatgtttgc
    48121 cggattaaca aacccgctgc cggtggcgcg ttatcactcg ctggttggca gtaacattcc
    48181 ggccggttta accatcaacg cccattttaa tggcatggtg atggcagtac gtcacgatgc
    48241 ggatcgcgtt tgtggattcc agttccatcc ggaatccatt ctcaccaccc agggcgctcg
    48301 cctgctggaa caaacgctgg cctgggcgca gcagaaacta gagccagcca acacgctgca
    48361 accgattctg gaaaaactgt atcaggcgca gacgcttagc caacaagaaa gccaccagct
    48421 gttttcagcg gtggtgcgtg gcgagctgaa gccggaacaa ctggcggcgg cgctggtgag
    48481 catgaaaatt cgcggtgagc acccgaacga gatcgccggg gcagcaaccg cgctactgga
    48541 aaacgcagcg ccgttcccgc gcccggatta tctgtttgct gatatcgtcg gtactggcgg
    48601 tgacggcagc aacagtatca atatttctac cgccagtgcg tttgtcgccg cggcctgtgg
    48661 gctgaaagtg gcgaaacacg gtaaccgcag cgtctccagt aaatccggct cgtcggatct
    48721 gctggcggca ttcggtatta atcttgatat gaacgccaat aaatcgcgcc aggcgctgga
    48781 cgagttaggc gtatgtttcc tctttgcgcc gaaatatcac accggattcc gccacgcgat
    48841 gccggttcgc cagcaactga aaacccgcac cctgttcaat gtgctggggc cattgattaa
    48901 cccggcgcat ccgccgctgg cgttaattgg tgtttatagt ccggaactgg tgctgccgat
    48961 tgccgaaacc ttgcgcgtgc tggggtatca acgcgcggcg gtggtgcaca gcggcgggat
    49021 ggatgaagtt tcattacacg cgccgacaat cgttgccgag ctgcatgacg gcaaaattaa
    49081 gagctatcaa ttgaccgctg aagattttgg cctgactccc taccaccagg agcaactggc
    49141 aggcggaaca cctgaagaaa accgtgacat tttaacacgc ttgttacaag gtaaaggcga
    49201 cgccgcccat gaagcagccg tcgctgcgaa cgtcgccatg ttaatgcgcc tgcatggcca
    49261 tgaagatctg caagccaatg cgcaaaccgt tcttgaggta ctgcgcagtg gttccgctta
    49321 cgacagagtt accgcgctgg cggcacgagg gtaaatgatg caaaccgttt tagcgaaaat
    49381 cgtcgcagac aaggcgattt gggtagaaac ccgcaaacag cagcaaccgc tggccagttt
    49441 tcagaatgag gttcagccga gcacacgaca tttttatgat gcgctacagg gtgcacgcac
    49501 ggcgtttatt ctggagtgta aaaaagcgtc gccgtcaaaa ggcgtgatcc gtgatgattt
    49561 cgatccggca cgcattgccg ccatttataa acattacgct tcggcaattt cggtgctgac
    49621 tgatgagaaa tattttcagg ggagctttga tttcctcctc atcgtcagcc aaatcgcccc
    49681 gcagccgatt ttatgtaaag acttcattat cgacccttac cagatctatc tggcgcgcta
    49741 ttaccaggcc gatgcctgct tattaatgct ttcagtactg gatgacgacc aatatcgcca
    49801 gcttgcagcc gtcgctcaca gtctggagat gggggtgctg accgaagtca gtaatgaaga
    49861 ggaactggag cgcgccattg cattaggggc aaaggtcgtt ggcatcaaca accgcgatct
    49921 gcgtgatttg tcgattgatc tcaaccgtac ccgcgagctt gcgccgaaac tggggcacaa
    49981 cgtaacggta atcagcgaat ccggcatcaa tacttacgct caggtgcgcg agttaagcca
    50041 cttcgctaac ggttttctga ttggttcggc gttgatggcc catgacgatt tgcacgccgc
    50101 cgtgcgccgg gtgttgctgg gtgagaataa agtatgtggc ctgacgcgtg ggcaagatgc
    50161 taaagcagct tatgacgcgg gcgcgattta cggtgggttg atttttgttg cgacatcacc
    50221 gcgttgcgtc aacgttgaac aggcgcagga agtgatggct gcggcaccgt tgcagtatgt
    50281 tggcgtgttc cgcaatcacg atattgccga tgtggtggac aaagctaagg tgttatcgct
    50341 ggcggcagtg caactgcatg gcaatgaaga tcagctgtat atcgatacgc tgcgtgaagc
    50401 tctgccagca catgttgcca tctggaaagc attaagcgtc ggtgaaaccc tgcccgcccg
    50461 cgagtttcag cacgttgata aatatgtttt agacaacggc cagggtggaa gcgggcaacg
    50521 ttttgactgg tcactattaa atggtcaatc gcttggcaac gttctgctgg cggggggctt
    50581 aggcgcagat aactgcgtgg aagcggcaca aaccggctgc gccggacttg attttaattc
    50641 tgctgtagag tcgcaaccgg gcatcaaaga cgcacgtctt ttggcctcgg ttttccagac
    50701 gctgcgcgca tattaaggaa aggaacaatg acaacattac ttaaccccta ttttggtgag
    50761 tttggcggca tgtacgtgcc acaaatcctg atgcctgctc tgcgccagct ggaagaagct
    50821 tttgtcagcg cgcaaaaaga tcctgaattt caagcccagt tcaacgacct gctgaaaaac
    50881 tatgccgggc gtccaaccgc actgaccaaa tgccagaaca ttacagccgg gacgaacacc
    50941 acgctgtatc tcaagcgtga agatttgctg cacggcggcg cacataaaac caaccaggtg
    51001 ctggggcagg cgttgctggc gaagcggatg ggtaaaaccg aaatcatcgc cgaaaccggg
    51061 gccggtcagc atggcgtggc gtcggccctt gccagcgccc tgctcggcct gaaatgccgt
    51121 atttatatgg gtgccaaaga cgttgaacgc cagtcgccta acgtttttcg tatgcgctta
    51181 atgggtgcgg aagtgatccc ggtacatagc ggttccgcca cgctgaaaga tgcctgtaat
    51241 gaggcgctac gcgactggtc cggcagttat gaaaccgcgc actatatgct gggcaccgcg
    51301 gctggcccgc atccttatcc gaccattgtc cgtgagtttc agcggatgat tggcgaagaa
    51361 accaaagcgc agattctgga aagagaaggt cgcctgccgg atgccgttat cgcctgtgtt
    51421 ggcggcggtt cgaatgccat cggcatgttt gctgatttca tcaatgaaac caacgtcggc
    51481 ctgattggtg tggagcctgg tggtcacggt atcgaaactg gcgagcacgg cgcaccgcta
    51541 aaacatggtc gcgtgggcat ttatttcggt atgaaagcgc cgatgatgca aaccgaagac
    51601 gggcagattg aagaatctta ctccatctcc gccggactgg atttcccgtc tgtcggtcca
    51661 caacacgcgt atcttaacag cactggacgc gctgattacg tgtctattac cgatgatgaa
    51721 gcccttgaag ccttcaaaac gctgtgcctg cacgaaggga tcatcccggc gctggaatcc
    51781 tcccacgccc tggcccatgc gctgaaaatg atgcgcgaaa acccggataa agagcagcta
    51841 ctggtggtta acctttccgg tcgcggcgat aaagacatct tcaccgttca cgatattttg
    51901 aaagcacgag gggaaatctg atggaacgct acgaatctct gtttgcccag ttgaaggagc
    51961 gcaaagaagg cgcattcgtt cctttcgtca cgctcggtga tccgggcatt gagcagtcat
    52021 tgaaaattat cgatacgcta attgaagccg gtgctgacgc gctggagtta ggtatcccct
    52081 tctccgaccc actggcggat ggcccgacca ttcaaaacgc cactctgcgc gcctttgcgg
    52141 caggtgtgac tccggcacaa tgttttgaaa tgctggcact gattcgccag aaacacccga
    52201 ccattcccat tggcctgttg atgtatgcca atctggtgtt taacaaaggc attgatgagt
    52261 tttatgccca gtgcgagaaa gtcggcgtcg attcggtgct ggttgccgat gtgccagttg
    52321 aagagtccgc gcccttccgc caggccgcgt tgcgccataa tgtcgcacct atcttcatct
    52381 gcccgccaaa tgccgatgac gacctgctgc gccagatagc ctcttacggt cgtggttaca
    52441 catatttgct gtcacgagcg ggcgtgaccg gcgcagaaaa ccgcgccgcg ttacccctca
    52501 atcatctggt tgcgaagctg aaagagtaca acgctgcacc tccattgcag gggtttggta
    52561 tttccgcccc ggatcaggta aaagcagcga ttgatgcagg agctgcgggc gcgatttctg
    52621 gttcggccat tgttaaaatc atcgagcaac atattaatga gccagagaaa atgctggcgg
    52681 cactgaaagt ttttgtacaa ccgatgaaag cggcgacgcg cagttaatcc cacagccgcc
    52741 agttccgctg gcggcatttt aactttcttt aatgaagccg gaaaaatcct aaattcattt
    52801 aatatttatc tttttaccgt ttcgcttacc ccggtcgaac gtcaacttac gtcatttttc
    52861 cgcccaacag taatataatc aaacaaatta atcccgcaac ataacaccag taaaatcaat
    52921 aattttctct aagtcacata ttcctcaggt aattgttaat atatccagaa tgttcctcaa
    52981 aatatatttc cctctatctt ctcgttgcgc ttaatttgac taattctcat tagtgactaa
    53041 ttttaatgag tgtcgacaca caacactcat attaatgaaa caatgcaacg caacgggaga
    53101 aataacatgg ccgaacatcg tggtagttca ggaaatttcg ccgaagaccg tgagaaggca
    53161 tccgacgcag gccgtaaagg cggtcagcat agcggcggta attttaaaaa tgatccgcaa
    53221 cgcgcatctg aagcgggtaa aaaaggcggt caacaaagcg gtggtaataa atcaggcaaa
    53281 tcctgattca ccgttaatta ttaatatcag ctgataaaga taattgcatc tgcgggccgt
    53341 atggctcgca gtgatttcac tggagaaaaa tatgaatatg aagaccattg aagatgtatt
    53401 tattcaactg ctttcagata cctacagcgc agaaaaacaa ttaacccggg cactggcaaa
    53461 actcgcaaga gcaacatcaa atgaaaaatt aagtcaggct tttcatgcgc acctcgagga
    53521 aactcatgga cagattgaac gaattgatca agttgtggaa tcagaatcaa atctgaaaat
    53581 taagcgcatg aaatgtgtgg caatggaagg tcttattgaa gaagctaatg aggtcatcga
    53641 aagtaccgag aaaaacgaag tgcgtgatgc cgcactgatt gccgcagcac agaaagtcga
    53701 gcattatgag attgccagtt acgggacatt agcgacgctg gctgaacaat taggttaccg
    53761 taaagcagcg aagcttctga aagaaaccct ggaagaagaa aaggccaccg acatcaaact
    53821 gactgatctg gcccttaata acgtaaataa gaaagccgaa aataaagcct gaaatatgaa
    53881 ttttaacttt tagtcatttt ataaagagga cattttcatg aatcgtattg aacattatca
    53941 tgactggtta cgtgacgccc acgcaatgga aaagcaagcc gaatctatgc ttgaatccat
    54001 ggccagccgt atagataatt atcctgaact acgcgctcgt attgaacaac atcttagtga
    54061 aaccaaaaac cagattgttc aactggaaac tattcttgat cgtaatgaca tttcacgttc
    54121 agtcattaaa gattccatga gtaaaatggc tgcgcttggg cagtcaatcg gtggtatatt
    54181 cccttctgat gaaatagtta aaggctctat tggcggatat gtcttcgagc aatttgaaat
    54241 cacctgttac acctcactat tagcagcagc aaaaaatgcc ggtgatacag tctcaattcc
    54301 gatcatcgaa gcgattttaa atgaggaaaa gcaaatggcc gactggctga ttcagcatat
    54361 tccgcaaaca actgagaaat ttttaattcg ttctgaaact gatggcgtag aagcgaagaa
    54421 ataataccct ttataccatg tccttattga ccccgtatat tacggggtcg tttttgtgcg
    54481 gaattaaaaa cgatatcctg ctgagaacat aaacacccac ggatcgaggc gtacgctatc
    54541 gtgttgctgt gcaccgccta gcttataatt ggcggtggta tcgatatcca tgtaccacac
    54601 tgacatgtta accaaccagt cacggttaat cagataatca acccccacct gcccggcagc
    54661 tccccaggaa tctttcagac tgagatcgga aagccccgcc tctttgccat gatcgttaaa
    54721 tccattatca aagaaggtgg tgtagttaat accggcccca acgtaaggac ggaatttgct
    54781 gctggcatca ccaaaatacc actgcgccat cagtgttggc ggcagatggt gaacggttgc
    54841 aatatcgccg gtcgcccggg tgccgatttt atggcggaac ggcgtcgctg ccagtaattc
    54901 cacaccaatg ttgtcggtcg ccatataagt aaacgtcaag cccagttgag tgttattggt
    54961 cacgctgaat ccacccagac ttcctaacgt accaccagca ccttctgttg gacgtacggt
    55021 tgcagaacct gcacgcataa aaaattcgcc tgcttcatgc gcaaaggcac tgccagaaag
    55081 aagagttgtt actgccaaag ccgccactgt taactttttc atatccgctc cgtcgttatg
    55141 gttataaaaa cgtgacgaat atacctacat tggagtaaca aatgatccta catagatcac
    55201 attacatatg ataattcacc atttattgat ctggattaat ttagaagttg cattgagaat
    55261 cctggttaat gtgatttaaa tcaattttta aatattttct ctacgcaaat attaactcga
    55321 tcgccacttg ccgcgctttg cccacgcctg aaggccagca attacctccg ctttacaaag
    55381 atatgctttt ccatacaaga cctgatgctg ccacccgagg cttaaccagg gtacaattgc
    55441 ccgctaatta acacctgcat aaaactcaag gagagtgcat gtctatcacg gcgcagtccg
    55501 tataccgtga caccggaaat ttcttccgta atcaatttat gaccattctg ttggtatcgt
    55561 tgctatgtgc gtttatcaca gtggtgttag ggcatgtttt ctcacccagt gatgcacagc
    55621 ttgcgcagct caatgacggc gtgcccgtta gcggcagtag tgggttgttc gacctggttc
    55681 agaatatgtc accggaacag caacaaattt tgctgcaggc ttcagcggcg tccacttttt
    55741 cagaattaat cggtaacgcc attctcgccg gaggcgtaat attaattatc cagctggtgt
    55801 ctgcgggtca gagagtcagt gcgctgcgag ctattggtgc cagtgcgccg atattgccca
    55861 agttatttat tctgattttt ctgactaccc ttttagtaca gattggcatc atgctggtgg
    55921 tcgttccggg aattatcatg gccattttac tggccctggc accggtgatg ctggtacagg
    55981 acaaaatggg catttttgcc tcgatgcgta gcagtatgcg gctgacttgg gcgaatatgc
    56041 gtctggtggc acccgcagta ctgagctggt tgctggcaaa aacactgttg ctgctttttg
    56101 cctcttcttt tgccgcatta accccggaaa ttggtgccgt actggcgaac accttgagca
    56161 acctgatttc tgccatattg ctcatctatc tgttccgcct gtatatgttg attcgccaat
    56221 aaccttaagc atttgattac ggaatcgtaa aatgaagcag tttcttgatt ttttaccgct
    56281 ggttgtcttt ttcgcgtttt acaagattta tgacatctat gcggctactg cagcgctgat
    56341 cgtcgccacg gcgattgtgc ttatctatag ctgggttcgc tttcgtaagg ttgagaagat
    56401 ggccctgatc acttttgttc tggtggtcgt cttcggtggc ttgacgctgt tcttccacaa
    56461 tgatgagttt attaaatgga aggttacagt catttatgct ctgtttgcgg gagccctgtt
    56521 agtcagccaa tgggtgatga aaaagccgct aattcagcgg atgctgagta aagaactcac
    56581 gctgccgcaa ccggtatggt cgaagctaaa tctggcctgg gctgttttct ttatcctttg
    56641 cggtctggca aacatctaca tcgcattctg gctgccgcaa aatatttggg tcaactttaa
    56701 agtctttggc ctgaccgccc ttaccttaat cttcacattg ttaagcggta tctatatcta
    56761 ccgccacatg ccgcaggaag ataaatccta accagactgc cagtccttcg gggctggcgc
    56821 tgtatctcct ctgttctcct caggaaaatc atagtagcat cgcgcctgtg attttccttt
    56881 taagtcggtt ttaccatgtc tacaacacat aacgtccctc agggcgatct tgttttacgt
    56941 actttagcca tgcccgccga taccaatgcc aatggtgaca tctttggtgg ttggttaatg
    57001 tcacaaatgg atattggcgg cgctattctg gcaaaagaaa ttgcccacgg tcgcgtagtg
    57061 actgtgcggg ttgaaggaat gactttctta cggccggttg cggtcggcga tgtggtgtgc
    57121 tgctatgcac gctgtgtcca gaaagggacg acatcggtca gcattaatat tgaagtgtgg
    57181 gtgaaaaaag tagcgtctga accaatcggg caacgctata aagcgacaga agcattattt
    57241 aagtatgtag cggttgatcc tgaaggaaaa cctcgcgcct tacctgttga gtaatagtca
    57301 aaagcctccg gtcggaggct tttgactttc tgcttactga atttcggtgg tgccgttaat
    57361 tttaaacagg atattcacca caatcccact gcctggctta cccggctcat aacgccatct
    57421 gcgcatcgca tttttcacct cacgctcaaa catgttcgca ggcttggctg agaggatttg
    57481 tacgttatcc acgcgaccat ccggcgtgac gtcaaattta actttaacct gcccttcaat
    57541 gcgcaatgcc tgtgctcgtg ccggatactg cggctgatta cggcttaatg cgcgtggtcc
    57601 tgaagccaca ctggtaaccg gcttgctggt tgcagccgtt gccgtacttg atgtggggcg
    57661 cgcgggtgcc gtattttcaa acggtgatgc cggacgcgac tctacgggtt tgacatcgcg
    57721 ttttggctgc tcctgtacct ttttcaccgg cttcggtttt ggcttaggtt tcggcttcgg
    57781 cttttcaatg accaccggtg cttcttttgg cggttcgggg atcggctcag gttccggttc
    57841 tggctctacc accggctccg gtggcggctg aacggcttgt ggcggttcga gatcagcagg
    57901 cgcaaccatc gtgacagaaa ttggttgcgc aggcgcaggt agttcaataa cctgatgtac
    57961 cgaggtatag agcagccccg ccacaacagc accatgaatg cagaccgaaa gtaacgtcgg
    58021 ccaggggaag cggcgaggta aatcaagggt tattgaagtc ataatcattt cagtagaaaa
    58081 actaggcctc gattttaaat gcaaatagca atcatattca ataaggcaag acgatcagga
    58141 tcagtgaaaa tgtcattttt ggcgtaatta cccttacttt atgcatgacc ttaacaaaga
    58201 catcatcttt ccgttgcaga tcttcgccct ttcaaataac gtactttaca actttcccga
    58261 acaaggagtt gtgcccgtgt tgtatgttat ttacgctcaa gacaaagctg atagcctcga
    58321 aaaacgcctt tccgttcgtc cggcacattt agcacgttta cagttactgc atgatgaagg
    58381 tcgtttgttg acagctggtc caatgccagc ggtagacagt aacgatccag gggctgcggg
    58441 atttactggc tcaaccgtta ttgctgaatt tgaatcgctg gaagcagctc aggcctgggc
    58501 cgatgctgac ccgtacgtag cggcaggagt ctatgaacac gtttcagtga aaccatttaa
    58561 gaaagtgttc tgag
//
LOCUS       DAZPCS010000008        55586 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZPCS010000008 DAZPCS010000000
VERSION     DAZPCS010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55586
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.008"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            170..1656
                     /locus_tag="ACHUQ7_000447"
                     /pseudo
     CDS             170..1656
                     /locus_tag="ACHUQ7_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
     gene            1671..2405
                     /gene="ypdB"
                     /locus_tag="ACHUQ7_000448"
     CDS             1671..2405
                     /gene="ypdB"
                     /locus_tag="ACHUQ7_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFR1134025.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            2418..3275
                     /locus_tag="ACHUQ7_000449"
                     /pseudo
     CDS             2418..3275
                     /locus_tag="ACHUQ7_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(3278..5780)
                     /gene="ptsP"
                     /locus_tag="ACHUQ7_000450"
                     /pseudo
     CDS             complement(3278..5780)
                     /gene="ptsP"
                     /locus_tag="ACHUQ7_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            complement(5805..6849)
                     /gene="ypdE"
                     /locus_tag="ACHUQ7_000451"
                     /pseudo
     CDS             complement(5805..6849)
                     /gene="ypdE"
                     /locus_tag="ACHUQ7_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(6849..7265)
                     /locus_tag="ACHUQ7_000452"
     CDS             complement(6849..7265)
                     /locus_tag="ACHUQ7_000452"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFR1134026.1"
                     /translation="MTRTLLVNGEGVSAESHPLFDVYQIVLQAQLAAISAIRPGVRCQ
                     QIDDAARRVITEAGFGDYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIY
                     LLGQGGVRIEDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            complement(7262..7369)
                     /locus_tag="ACHUQ7_000453"
     CDS             complement(7262..7369)
                     /locus_tag="ACHUQ7_000453"
                     /inference="COORDINATES: protein motif:HMM:NF019360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1134027.1"
                     /translation="MARGAAARQSQRQDCCSGRVCHSRFWCAVSGLLLI"
     gene            complement(7330..7932)
                     /gene="ypdF"
                     /locus_tag="ACHUQ7_000454"
                     /pseudo
     CDS             complement(7330..7932)
                     /gene="ypdF"
                     /locus_tag="ACHUQ7_000454"
                     /inference="COORDINATES: protein motif:HMM:NF007310.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(7947..9194)
                     /locus_tag="ACHUQ7_000455"
     CDS             complement(7947..9194)
                     /locus_tag="ACHUQ7_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFR1134028.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(9216..9542)
                     /locus_tag="ACHUQ7_000456"
     CDS             complement(9216..9542)
                     /locus_tag="ACHUQ7_000456"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFR1134029.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQVLEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(9761..10726)
                     /gene="glk"
                     /locus_tag="ACHUQ7_000457"
     CDS             complement(9761..10726)
                     /gene="glk"
                     /locus_tag="ACHUQ7_000457"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFR1134030.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            10930..12186
                     /locus_tag="ACHUQ7_000458"
     CDS             10930..12186
                     /locus_tag="ACHUQ7_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFR1134031.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPR
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            12301..12627
                     /gene="ypeC"
                     /locus_tag="ACHUQ7_000459"
     CDS             12301..12627
                     /gene="ypeC"
                     /locus_tag="ACHUQ7_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFR1134032.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(12768..14006)
                     /locus_tag="ACHUQ7_000460"
     CDS             complement(12768..14006)
                     /locus_tag="ACHUQ7_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFR1134033.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLVMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            14342..15544
                     /gene="nupC"
                     /locus_tag="ACHUQ7_000461"
     CDS             14342..15544
                     /gene="nupC"
                     /locus_tag="ACHUQ7_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFR1134034.1"
                     /translation="MDRVLHFVLALAVVVILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(15594..17706)
                     /locus_tag="ACHUQ7_000462"
                     /pseudo
     CDS             complement(15594..17706)
                     /locus_tag="ACHUQ7_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(17914..17989)
                     /locus_tag="ACHUQ7_000463"
     tRNA            complement(17914..17989)
                     /locus_tag="ACHUQ7_000463"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17954..17956),aa:Ala,seq:ggc)
     gene            complement(18029..18104)
                     /locus_tag="ACHUQ7_000464"
     tRNA            complement(18029..18104)
                     /locus_tag="ACHUQ7_000464"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18069..18071),aa:Ala,seq:ggc)
     gene            18325..18684
                     /locus_tag="ACHUQ7_000465"
     CDS             18325..18684
                     /locus_tag="ACHUQ7_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFR1134035.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            18686..19078
                     /locus_tag="ACHUQ7_000466"
     CDS             18686..19078
                     /locus_tag="ACHUQ7_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFR1134036.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWTREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(19130..20545)
                     /gene="gltX"
                     /locus_tag="ACHUQ7_000467"
     CDS             complement(19130..20545)
                     /gene="gltX"
                     /locus_tag="ACHUQ7_000467"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFR1134037.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            20804..20879
                     /locus_tag="ACHUQ7_000468"
     tRNA            20804..20879
                     /locus_tag="ACHUQ7_000468"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20837..20839,aa:Val,seq:tac)
     gene            20924..20999
                     /locus_tag="ACHUQ7_000469"
     tRNA            20924..20999
                     /locus_tag="ACHUQ7_000469"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20957..20959,aa:Val,seq:tac)
     gene            21004..21079
                     /locus_tag="ACHUQ7_000470"
     tRNA            21004..21079
                     /locus_tag="ACHUQ7_000470"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21037..21039,aa:Lys,seq:ttt)
     gene            21110..21235
                     /locus_tag="ACHUQ7_000471"
     ncRNA           21110..21235
                     /ncRNA_class="other"
                     /locus_tag="ACHUQ7_000471"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            21355..21696
                     /locus_tag="ACHUQ7_000472"
     CDS             21355..21696
                     /locus_tag="ACHUQ7_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HFR1134038.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(21687..22613)
                     /locus_tag="ACHUQ7_000473"
     CDS             complement(21687..22613)
                     /locus_tag="ACHUQ7_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1134039.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            22703..23701
                     /locus_tag="ACHUQ7_000474"
     CDS             22703..23701
                     /locus_tag="ACHUQ7_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFR1134040.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLSFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(23698..23916)
                     /locus_tag="ACHUQ7_000475"
     CDS             complement(23698..23916)
                     /locus_tag="ACHUQ7_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFR1134041.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(23918..25933)
                     /gene="ligA"
                     /locus_tag="ACHUQ7_000476"
     CDS             complement(23918..25933)
                     /gene="ligA"
                     /locus_tag="ACHUQ7_000476"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFR1134042.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGVPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(26004..26990)
                     /gene="zipA"
                     /locus_tag="ACHUQ7_000477"
     CDS             complement(26004..26990)
                     /gene="zipA"
                     /locus_tag="ACHUQ7_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFR1134043.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYLPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            27220..27981
                     /gene="cysZ"
                     /locus_tag="ACHUQ7_000478"
     CDS             27220..27981
                     /gene="cysZ"
                     /locus_tag="ACHUQ7_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFR1134044.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFVWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            28166..29137
                     /gene="cysK"
                     /locus_tag="ACHUQ7_000479"
     CDS             28166..29137
                     /gene="cysK"
                     /locus_tag="ACHUQ7_000479"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFR1134045.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            29521..29778
                     /gene="ptsH"
                     /locus_tag="ACHUQ7_000480"
     CDS             29521..29778
                     /gene="ptsH"
                     /locus_tag="ACHUQ7_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFR1134046.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            29823..31550
                     /gene="ptsI"
                     /locus_tag="ACHUQ7_000481"
     CDS             29823..31550
                     /gene="ptsI"
                     /locus_tag="ACHUQ7_000481"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFR1134047.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            31591..32100
                     /gene="crr"
                     /locus_tag="ACHUQ7_000482"
     CDS             31591..32100
                     /gene="crr"
                     /locus_tag="ACHUQ7_000482"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFR1134048.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(32142..32993)
                     /gene="pdxK"
                     /locus_tag="ACHUQ7_000483"
                     /gene_synonym="thiJ"
     CDS             complement(32142..32993)
                     /gene="pdxK"
                     /locus_tag="ACHUQ7_000483"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFR1134049.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            33098..33472
                     /locus_tag="ACHUQ7_000484"
     CDS             33098..33472
                     /locus_tag="ACHUQ7_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFR1134050.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            33505..34228
                     /locus_tag="ACHUQ7_000485"
                     /pseudo
     CDS             33505..34228
                     /locus_tag="ACHUQ7_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(34432..35343)
                     /gene="cysM"
                     /locus_tag="ACHUQ7_000486"
     CDS             complement(34432..35343)
                     /gene="cysM"
                     /locus_tag="ACHUQ7_000486"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFR1134051.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSCFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNAALVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(35477..36574)
                     /gene="cysA"
                     /locus_tag="ACHUQ7_000487"
     CDS             complement(35477..36574)
                     /gene="cysA"
                     /locus_tag="ACHUQ7_000487"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFR1134052.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQHGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(36564..37439)
                     /gene="cysW"
                     /locus_tag="ACHUQ7_000488"
     CDS             complement(36564..37439)
                     /gene="cysW"
                     /locus_tag="ACHUQ7_000488"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFR1134053.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(37439..38272)
                     /gene="cysT"
                     /locus_tag="ACHUQ7_000489"
     CDS             complement(37439..38272)
                     /gene="cysT"
                     /locus_tag="ACHUQ7_000489"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFR1134054.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(38272..39288)
                     /gene="cysP"
                     /locus_tag="ACHUQ7_000490"
     CDS             complement(38272..39288)
                     /gene="cysP"
                     /locus_tag="ACHUQ7_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFR1134055.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(39592..40383)
                     /gene="ucpA"
                     /locus_tag="ACHUQ7_000491"
     CDS             complement(39592..40383)
                     /gene="ucpA"
                     /locus_tag="ACHUQ7_000491"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFR1134056.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(40512..41370)
                     /gene="murR"
                     /locus_tag="ACHUQ7_000492"
                     /pseudo
     CDS             complement(40512..41370)
                     /gene="murR"
                     /locus_tag="ACHUQ7_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
     gene            41534..42430
                     /gene="murQ"
                     /locus_tag="ACHUQ7_000493"
     CDS             41534..42430
                     /gene="murQ"
                     /locus_tag="ACHUQ7_000493"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFR1134057.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPPAVDRV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            42434..43858
                     /gene="murP"
                     /locus_tag="ACHUQ7_000494"
     CDS             42434..43858
                     /gene="murP"
                     /locus_tag="ACHUQ7_000494"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HFR1134058.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGDAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            43863..43949
                     /locus_tag="ACHUQ7_000495"
                     /pseudo
     CDS             43863..43949
                     /locus_tag="ACHUQ7_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(44038..44937)
                     /gene="yfeX"
                     /locus_tag="ACHUQ7_000496"
     CDS             complement(44038..44937)
                     /gene="yfeX"
                     /locus_tag="ACHUQ7_000496"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFR1134059.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDSGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(45033..45608)
                     /locus_tag="ACHUQ7_000497"
     CDS             complement(45033..45608)
                     /locus_tag="ACHUQ7_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFR1134060.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTTNGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(45669..46118)
                     /locus_tag="ACHUQ7_000498"
     CDS             complement(45669..46118)
                     /locus_tag="ACHUQ7_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFR1134061.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(46105..46530)
                     /locus_tag="ACHUQ7_000499"
     CDS             complement(46105..46530)
                     /locus_tag="ACHUQ7_000499"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFR1134062.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            46744..47613
                     /gene="amiA"
                     /locus_tag="ACHUQ7_000500"
     CDS             46744..47613
                     /gene="amiA"
                     /locus_tag="ACHUQ7_000500"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFR1134063.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            47617..48516
                     /gene="hemF"
                     /locus_tag="ACHUQ7_000501"
     CDS             47617..48516
                     /gene="hemF"
                     /locus_tag="ACHUQ7_000501"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFR1134064.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(48522..49574)
                     /gene="eutR"
                     /locus_tag="ACHUQ7_000502"
                     /pseudo
     CDS             complement(48522..49574)
                     /gene="eutR"
                     /locus_tag="ACHUQ7_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
     gene            complement(49620..50120)
                     /gene="eutK"
                     /locus_tag="ACHUQ7_000503"
     CDS             complement(49620..50120)
                     /gene="eutK"
                     /locus_tag="ACHUQ7_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFR1134065.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(50133..50792)
                     /gene="eutL"
                     /locus_tag="ACHUQ7_000504"
     CDS             complement(50133..50792)
                     /gene="eutL"
                     /locus_tag="ACHUQ7_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFR1134066.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(50802..50870)
                     /locus_tag="ACHUQ7_000505"
                     /pseudo
     CDS             complement(50802..50870)
                     /locus_tag="ACHUQ7_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311338.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            51974..52765
                     /locus_tag="ACHUQ7_000506"
     CDS             51974..52765
                     /locus_tag="ACHUQ7_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1134067.1"
                     /translation="MRALKIDAISLNFSHSPEWWRSGLIATLPRLDIERIIDTYIKRK
                     RDKFIVKIRGLHIMRISEQQRQILDYLAKNESITARQAADLVYGGNVTRVQVDAARRS
                     LMTMVSNGLIRKQGRAFVAERHINHAYSQDVEELRRQILWVFSKQGQEYFLSEHPNFA
                     AKELTVSGLSCALDYLVGEGCVTYKQLWHALNTLVEDGLLIATRENIEYWIMPRSGRR
                     NHARYISGNRYRTPDFNPTQEDRDRAEMMCREQEKRIMDKLFSMQ"
     gene            53485..53880
                     /locus_tag="ACHUQ7_000507"
     CDS             53485..53880
                     /locus_tag="ACHUQ7_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFR1134068.1"
                     /translation="MTNIERLERCADIMRRRWIYDPEAGLLFSRETKDVIKGSMTNKG
                     HLVVTVHEGKFKVTLTYQKACYVYTYGAYDETLYEVHHVNLNKQDNRIKNIRLMPKEK
                     HRRIHKNVRKLIRLGHTQWLQPQLIEQSL"
     gene            53898..54161
                     /locus_tag="ACHUQ7_000508"
     CDS             53898..54161
                     /locus_tag="ACHUQ7_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1134069.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFHEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKRRLICELSRRFGVSISAFK"
     gene            54184..54726
                     /locus_tag="ACHUQ7_000509"
     CDS             54184..54726
                     /locus_tag="ACHUQ7_000509"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1134070.1"
                     /translation="MDAFEVLANKGAELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIETIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMYKQSGAPNPDEYFSPD
                     VEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     ATDPEAEQRKRVFNAIGIRY"
     gene            54865..55152
                     /locus_tag="ACHUQ7_000510"
     CDS             54865..55152
                     /locus_tag="ACHUQ7_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1134071.1"
                     /translation="MLALRIMQGIAKTLAEHVLDLKHSPLSKQAMKRQTLRLWAEYSL
                     GTINKIIDMKSGPSNQSAEEMEFIRRLILIRRDIHSQLHSVGIDINDGTGD"
     gene            55191..55397
                     /locus_tag="ACHUQ7_000511"
     CDS             55191..55397
                     /locus_tag="ACHUQ7_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1134072.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
BASE COUNT        13877 a        14746 c        13735 g        13228 t
ORIGIN      
        1 tttcaataaa tttaccagcc agaagtgcat tatcaagaga tacattgcta atacaccatt
       61 tatcttttaa tcccctttta aatttatgtt ttttcctcaa aaacggcctg ctggtcatca
      121 gccttcaggt tacctatcat agaggtttaa tccttattca gagtcacccg tgcacgaaat
      181 attcaacatg ctgctggcgg tcttcgatcg ggcagcgtta atgcttatct gcctgttctt
      241 tctcatccgt atccgcctgt ttcgcgaact gttgcacaag tcggcgcact ccccaaaaga
      301 attgctcgcc gttaccgcca ttttctcgct gttcgccctg ttcagcacct ggtccggcgt
      361 tcccgtagaa ggctcgctgg tgaacgtacg tattatcgcg gtgatgtccg gcgggattct
      421 gtttggcccg tgggtaggca tcattaccgg cgtgattgcg ggtattcacc ggtatttaat
      481 tgatatcggc ggcgtgacgg cgatcccctg ctttatcacc agcattctgg cgggttgtat
      541 atcaggctgg ataaacctga aaatccccaa agcacagcgc tggcgctgga tatcgccaac
      601 aaaacgctac cgctgtttcg ccatgtcaat agcgagtcat tacgcaaggt ctgcgaaatt
      661 atccgcgatg acattcacgc cgatgcggtg gcgattacta ataccgatca tgtgctggcc
      721 tatgttggcg tgggtgaaca taactatcag aatggcgatg acttcattag cccgactacc
      781 cgtcaggcga tgaattacgg aaaaatcatc attaaaaaca atgatgaagc ccaccgcaca
      841 ccagagattc attccatgct ggtgatccca ttgtgggaga aaggggtcgt gaccggaacg
      901 ctgaaaattt actactgcca cgcgcatcag atcacctcgt cattacagga aatggcggtc
      961 ggtctgtcgc aaatcatctc cacgcaactg gaggtttcac gcgccgagca gctacgtgaa
     1021 atggcaaata aggcagagat tcgcgccctg caaagcaaaa ttaatcccca ttttctgttt
     1081 aacgctctga acgctatttc atcgtcaatc cgtctgaatc cggataccgc tcgccagttg
     1141 atctttaatc tgtcgcgtta tctgcgctat aacattgaat taaaagacga tgagcaaatc
     1201 gatatcaaaa aagagctgta tcaaattaaa gactatattg ccattgagca ggcccgcttt
     1261 ggcgacaagc tgacggttat ctatgatatt gatgaagagg tgaattgctg cattcccagc
     1321 ctgctgatcc agccgttggt ggagaacgcc attgtccacg gtattcagcc ttgcaaaggt
     1381 aaaggcgttg tcaccatcag cgttgcagag tgcggaaatc gggtacgcat tgcggtgcga
     1441 gataccgggc acggcatcgt tccaaaggtg attgagcggg tcgaagcgaa tgaaatgccg
     1501 ggcaataaaa ttggcctgct gaatgtccat catcgcgtga agttattgta tggcgagggg
     1561 ctgcatatcc gccgcctgga gccggggacg gaaattgcgt tttacattcc taaccaacgc
     1621 accccagtcg cctcacaggc tacgttattg ctttgagccg gagtgatatt gtgaaagtca
     1681 tcattgttga agacgaattc ctggcacaac aggaactgag ctggctaatt aaagagcaca
     1741 gccagatgga gattgtcggc acctttgacg acggtctgga cgtgttgaag tttttgcagc
     1801 ataaccgcgt cgacgccatt tttctggata tcaatattcc gtcgctggat ggcgtgttgc
     1861 tggcgcaaaa catcagccag ttcgcccata aaccgtttat tgtgttcatc accgcgtgga
     1921 aagaacatgc ggtagaagcg tttgaactgg aggcgtttga ctacattctc aaaccgtatc
     1981 aggagtcacg tattaccggg atgctgcaaa aactggaagc ggcctggcaa caacagcaga
     2041 ccagcagtac gcctgccgcg acggtaacgc gtgagaatga caccattaat ctggtgaaag
     2101 atgagcgaat aatcgtcacg ccaattaacg atatctatta cgccgaagcg cacgagaaaa
     2161 tgacctttgt ctatacgcgg cgtgaatcct acgtaatgcc gatgaacatt accgaatttt
     2221 gcagcaaact gccgccgtcg cattttttcc gctgccatcg ctcattttgt gtcaatctga
     2281 acaaaatacg cgaaatcgaa ccgtggttta ataacaccta cattctgcga ctgaaagatc
     2341 tggattttga agtgccggtc agccgcagca aagtgaaaga atttcgccag ttaatgcatc
     2401 tttaatcggg aactttcatg aaagcaccag gcttgcccgc cgatcaacaa tttttcgccg
     2461 atctgttcag cggcctagtg cttaacccgc aactactggg gcgcgtctgg tttgccagcc
     2521 agcctgcctc gttaccggtg ggcagtttat gtattgattt tccccgtctg gatatcgtgc
     2581 tgcgcggcga atacggcaac ctgctggaag caaagcagca gcgaatggtg gaaggagaaa
     2641 tgctgtttat tccggcacgc gcggctaatt taccgatcaa caacaaaccg gtgatgctgt
     2701 taagcctggt gttcgcaccg acctggcttg ggttatcgtt ttacgatagc cgcaccacgt
     2761 cgttgttgca tcctgctcgc cagacccagc ttcccagcct gcaacgcggt gaaggtgaag
     2821 cgatgcttac cgccctcacc catcttagcc gttcgccgct ggagcagaat atcattcagc
     2881 cactggtgtt aagtttgctg catctttgcc gcaacgtggt gaatatgccg ccgggtaatt
     2941 cacaaccgcg cggcgatttt ctctatcaca gcatttgtaa ctgggtttag gataattatg
     3001 cccagccgct cacccgcgag agcgtggcgc agttttttaa tatcacgccc aatcatctgt
     3061 caaaactgtt tgctcagcat ggaacgatgc gttttatcga gtatgtgcgt tgggtgcgaa
     3121 tggcgaaggc gaggatgatt ttgcagaaat atcatctgtc aattcatgaa gtggcacagc
     3181 gttgcggttt tccggatagc gactattttt gtcgcgtttt ccggcgtcag tttagtctga
     3241 cgccgggaga gtacagcgcc cgttttcagg gctaacgtca gaaggttaat tctgtttcca
     3301 gcagcgtcag gatactttgt gcatcttgcg cggcaaacaa cgactggcgg aagtttttat
     3361 tcaccagttt acgcgccagc tgcgagaaga ctttcacatg attcatccct tcgttagcac
     3421 ccagcgtcag catgatcacc agttcgactt cgcccatttc tgactgccag tcaaccggtt
     3481 tcgccagccg ggcaatgctg atactggaat gacggatcca ctgagatttg gtgtgcggga
     3541 tcgctacgcc aaaaccaacg ccggtggtaa caatctcttc ccgctgccag acatcttctt
     3601 ccagctcaaa cggatgttca gtgcgcccgt taacgccgag gttgccgcac aggaactgga
     3661 tcgcctgctc tttattgcta aaatcctgat caacaaagat attctccagc gccagcagtg
     3721 ggcgaacgtc ttcttccggc gtaaaggcgg tgagtaacgc ttcaatttcc tgcgcactgc
     3781 ggcattcaca tgcctgacgc gccagttccc gacacgcctc gctatccagt tgacgaagct
     3841 ggcttttcac cgccggaata cgcgggctac tcatactcag ctcatccagg cccagcccaa
     3901 gcagtagcgg cagataacgg ctttcaccgc ccagttcacc gcaaatgcct acccatttgc
     3961 cccgctgatg cgcagtggta actatttgct gcaacatgcg caggaacgat ggcgtaatcg
     4021 ggttatatag cggcgatacg cgcgggttat tacgatcgac cgcatacaga tactgggtca
     4081 tatcgttgga gccgatactg aagaaatcga cctcatcgca gaagtggtcg atgatgtagc
     4141 acaccgacgg cacttccacc atgatcccaa gcgtaatcgt ctctgcatga cgcaggccat
     4201 cgcgcttaag ctcaacgatc gctttttgga tctcgccttt cacccataag atctgatcga
     4261 ggctgtgaac catcgggatc atcaactggg cgttgccgaa actggcggcg cgcaaaatgg
     4321 cccgcagttg agtgcggaac aggccagcaa attccgggta aatacgtacc gcgcgatagc
     4381 cgaggaacgg gttctcttcc tggggaatat tcagataagg aatgctttta tcgccgccga
     4441 tgtccatcgt gcggaagata atcggcttgt cgcccgccgc ccgccgccag tagcacctgc
     4501 tggtaggctt caaattgctc ctgctcgtcc ggcgcgctgt cgcgatccat atagagcatt
     4561 tcggtacgga acagcccgac accttccgcg ccgttggcaa acgcgcctgg cgcttccaga
     4621 gcggtgccga tattcgccgc aatatcaata cgcttgttat cacgggaata ggccagctgc
     4681 gcggcagcct gcgcctgttg tttttggcgt ttatccgcca gcgtctgcgc gacctgataa
     4741 taaccgctca ccgcgtcatt cgggttaatc gccagcacgc cgcactgggc gtcaagcact
     4801 gcaggttgcc cggcataacg ggcaatcgca tccagcggca agccactcag taccgggatc
     4861 gccgaggcac gggccagaat cagtgtatgc gaggtgcgcc cggttttctc cagaatcata
     4921 cccgcaagat ttttcaaatc aaggctcaaa aactggcttg gggttaaatc ttcagccacc
     4981 agaatggtcg gtttttccag caccagattg ttgcgcggct tcagttccgg ccaggtgata
     5041 tgcagcaact gttcgctgat atcgcgaatg tcgctgaccc gctcgcgcag ataatcgctg
     5101 gcagaagcag atagtttggc gcaaacctgc tccatattgc tgatgatcgc cgcccccagt
     5161 ccctgatgct gttctgtcat caggcgacgg atattgcctg caaattcatc atcctgaatc
     5221 agcgacaaat gagcgctgag gatagttttg ctttcgccgt cacgctcacg caattgctga
     5281 ttcagttgct cggcaagcgt tgccaggctg tgctccagcc gggtggaatc ttgcgcactg
     5341 gcggggattg cccgataact gtcgaggctg tcgctttgta acagggtcag cgtacccacg
     5401 ccgacgccgc ttgccagcac attgccgtac agtaaatccg ggttaaggcg gcttaatgaa
     5461 cgcggcagcg gatgcgccgt cagttctgcc tgcgtcggct gaacgctgtc gctgtcgata
     5521 aagcgcacca ggatgtactc ttccagcacg cgccgcgcct gctcttcatc gctgccgcta
     5581 atgttcaggc tgcaactgtc attaaataag gtgccagttc caatcaacgc cagcgagctt
     5641 ttagcatcag cctttgcgtt ctggcgatgg ttgataaagg tgatttcgct ttgccactgg
     5701 ctgcactgtt ctttaagttc ccacgccgga cgagcatgta gaccgttagg caaaggacag
     5761 agaaattgaa tcgttaacat aaccgctccg ggttaatcag gagatcatct gaaatccgtc
     5821 agttgaacaa ccgtctcacg cgtaagacgt tgaataaggg cagataaaag ttgctccatc
     5881 tgcaaaatgt cgcggcaatc ggcaatcggc aatcgatgcc gcgcaatgtc cgtggcgggt
     5941 ggcaggcccc attaccaatg tgggtacgcc agtaccggtt aagtgcaccg ctccaccgtc
     6001 cgtgccgccg ttactgaaca tatccgcctg caacggaacg ccaatttctg ccgccacggt
     6061 ttcaatccac gcggtaagtt ttggcggcgc aatcagcgat ttatcgctta acaccagcat
     6121 cggcccgtta ccaatctgac gatggttagc cgcgccataa tcaaagtttt tcgcccagca
     6181 ggcggtatca agcacaatgg cgacgtccgg cgacaccgcg cgggtagcag tttgcccgcc
     6241 gcgtaatccc acctcttcgc tggaacttgc caccagccac acttccgcag gcagttcggc
     6301 gtcgtgcagc tcacgtagta acgtcaccag cagatagcaa ctgaggcgat catcaaaggc
     6361 tttgcccatc acccgctggt gagggaggac ctgaaaagtg gtatcgaagg tgacgcgatc
     6421 gcctggacga attcccgcct gcatcacttc gtcataggtg cgcgcgccaa tgtccacgcg
     6481 catggcgcta acgtcattcc cctgccggtc gccgtcaagc aggcctggaa ttttgcactc
     6541 ttcacgggtg gtgatgcgca ccggctgcag ctggcgggca gccatgcgca cgttgccaac
     6601 cggcagcacg tcaatagcgc cttcgcggga gatgctgcgc accataaatc ccacttcgtc
     6661 catatgggcg cagatcatca ccttcggacc tgtcgattca ttgaggcgga tcagcaccga
     6721 tcccagacca tcaaatcgca cttctttttg caggcgagcc gcttcttcca gcaggatctg
     6781 ccgcacttcc tgttccgagg aggcgatcgc atctgcctcg ctcaacgctt ttaatagcga
     6841 taaatccatt atgcctctcc cgtgagcaac actgttttcg gcatggcgta gagcacttct
     6901 gcgccttgcg gggtaaccag cacaacatct tcgatgcgca cgcccccttg ccctagcaaa
     6961 taaatccccg gctccacggt cagcaacatg cctggctgta gcgtcgtggt gtcccgcggt
     7021 gaaaaacgcg gatcttcatg gacttcaatg ccgatagcgt gaccagtgtt atgaccgaaa
     7081 tagtcgccaa atccagcctc ggtaatgacc cggcgcgcgg cgtcgtcaat ctgctggcag
     7141 cgcacgccgg ggcgaattgc agagattgcc gcgagctgtg cctgcaggac aatctgatag
     7201 acgtcaaaca gcgggtgaga ttcggcgctc accccttcgc cattcaccag caaggtgcgc
     7261 gtcatatcag cagtagccct gatacagcgc accaaaatcg agagtgacaa actcgcccgc
     7321 tgcaacaatc ttgtcgctgg ctttgccgtg cggcagcgcc ccgcgccagc cgctggcgac
     7381 aatggtgtca aaagaggctt tttctgcgcc ctgctggcgc ataaaccact ccagttcagc
     7441 ggctatctcg cgctcgctca tccccgcctg aataaagcgg cgaatatgct ccgctccgcg
     7501 atcggcaatc ccacaggcga ggcggattat ctccacctcc tctggcgttt tgatttgccg
     7561 cagcacatcc ggcgtggcgc taaccagttt cgcattgagt tcagacttcc agcggtgcgc
     7621 ggtttcccaa ctcacctgct ggccttcaaa accgagcgtt tgcaactgct catcggcaat
     7681 gatttgattg acgatagtgg taagcgtgtt cgttgcgtca agcaaatgca gctggtagcc
     7741 ttgcgcacgg gcttccacct caacgaaata gcgcgaatct accagaatgt gcgcactttc
     7801 acggctaata accacatagc ctgatccggt ggagatcccc agatgcggct gtttgttctg
     7861 ccgtgaggag agaagcaccg catccagttg ttgcacctta agccagttgc gcagcgaagc
     7921 gagtaatgtc atctggattt tccttcttac aggctatcga ttaacaattt gcctttgcgg
     7981 aacatcatca gacgcaggaa caccaccatc agcgcagtaa tgattgcccc cagcgcgatc
     8041 cccgccatat aaacgcccaa gttagtgacc agcggccatg cccagatagc ggattccggg
     8101 aaccattgca ctgcgcccag ccagacagct gcggttgagc cgacaatcgc gccgaccata
     8161 tatgacggaa tagcggtgat ggggctttcc agcgcaaacg gaatcgcacc ctcactaatc
     8221 cccataaagg ccaggaacat cgcggtctta ccctgcggat aaagttgagc gttgaacagg
     8281 cgtttgccgg ttaaacgacg gtcaataatg gtcgccagac ccagaccaat cggcggaata
     8341 acgatagcga tagaacgagc ggtgaccggt aatacgtggt cagtggtaaa gctgaaggca
     8401 acgaaacctg cggctttgtt gatcgggcca ccaaggtcga ttgctgtcgc cgcggcgatc
     8461 cccattgcgt acatcagcgc gcccttctca cctgcggcag tcagcacagt acggataccg
     8521 ccgttgatcc agccaccaaa gggagtgatg acgtagtaca tcgccagcat gacaaaaatg
     8581 gcggaaagaa tcggtagcaa aaatgtggtt ttgaacgcta acaggaaatc cggtaactgg
     8641 attttctggt tcatccattt cacaaggtaa ccggcaacga tagaaataat cagcgcacca
     8701 atgaaggtgg acggcaccgg cgaagaggtc gcccattgca tagtgctggg atcgaagttc
     8761 agcagttgcg tcggctgggt agacatcaac ccgccgataa aaccagccgg gaacgccagt
     8821 ttgccaccga tagagttcgc cacaaatgca gcaaacatcg gaatggcaaa accaaacaac
     8881 acgccgccga aggactgcga cagccaggca aatttaagta aggagaggtc gaaaccggag
     8941 aatttcccgc tattaagagc atccatgatg ccgatctcag caggaatttt aagccagcta
     9001 taagcaatca gctggctgaa agcgaggatc acaccgccca taattaaggt cggcaccatg
     9061 cgtgaaatcc ctgacatcac atgctgcggg agttcacccc agaagctgct tttagaggcc
     9121 tgcggattct taactgccgc tgccgcaccg gatgcgccag gcacaacggt tgcactgcgt
     9181 tttttaatgg ccataacaat tatccctgtc gaatattact gttgttcaga ggcaatcatc
     9241 tcttcgattt ctttgatgat gcccgcagcg tttttaattg cgtcctgcaa agtgatttca
     9301 taaacgtcgc gtgattcgaa acgttcgtta tcttccgggg taactgccac ggaatggatg
     9361 atgatggtcg cttcggcgat atcctgcgcc gtcaggcgat tctggatgcc gtccgcgccc
     9421 tgggtttcaa ttttcacttc ataaccggct tctaccgccg cttcttccag cacctgagcg
     9481 gccataaaag tgtgagccag gcccattggg caggcacata aggcaatcag tttcttactc
     9541 atcgttaatt cctcgttcag tgatagttga ctagtgaaat tatgaacgtg caaaacaaat
     9601 cgccgttacc agacaaataa tgcatttact ggagaattaa tcccgctgta cggaagtgtg
     9661 acgcaggtcg gccttgtgag gggacacaaa gaaaaattga attaactgta tgatttaaaa
     9721 gattatcggg agagttacct cccgatataa caggaaggat ttacagaatg tgacctaagg
     9781 tctggcgtaa atgtgcaccg gaaccgagaa gacccggata gtcatggacg atgagataca
     9841 ccggaatatc atggacatat tctttaaagc gccctttatc ttcaaatgcg gcacggaaac
     9901 cggaggcttt gaagaactca aggaagcgcg gcacgatacc gcccgcaata aacacgccgc
     9961 caaatgtccc gagattgagc gccagattgc cgccaaaacg gcccataatg acgcaaaaca
    10021 gcgacaatgc gcggcggcaa tcggtgcagc tgtcagccag cgcgcgttcg gtaatatctt
    10081 ttggcttgag attttctggc aggcggttgt cagctttcac aattgcgcga tacaaattca
    10141 ccagcccagg gccagaaagc acgcgctccg ccgaaacatg accaatttcc gcacgcaata
    10201 tttcgaggat aatggcctct tcttcactat tcggcgcaaa atcaacgtga ccgccttcgc
    10261 ccggcaagct tacccaacgc ttatcgacat ggaccagatg cgcaacccca agccccgttc
    10321 cggcaccgta aaccgcaata ggcttacctt cgaccggttc tgcgccacca aactgaatca
    10381 gatgctcttt tttcagcatc gggatcgcca tcgatacagc ggtaaaatcg ttaataattt
    10441 ccagatggct aaaaccgaga ttctttttca tttcggcaat tgagaacgcc caggtatggt
    10501 tggtcattgc cacccagtca ccggtaattg ggcaagcgat ggcaatacag ccgtctttca
    10561 cctcgacctt atgttcttca agataaacgc gaatgaccgc ttcgaggctg gggtaatcaa
    10621 gccctgaata ggtcttagcc tgcgagattt caccactggc aatatcacac agagcaagac
    10681 gtgcgttggt gccgcccaca tcaccgacta atgcatactt tgtcattctt caactgctcc
    10741 gctaaagtca aaataattct ttctcacact gtaaatacct gggggcataa caacaacgcc
    10801 ggaaaggcag gctccctgta aatatcgatc tgggtcacac aattacttta tcgtttcagc
    10861 accaattgca gcgatgcctt tttgcaagct gggcaaacta agtatctgac cccgcataag
    10921 gaatagaaca tgctccatcc gcgagccaga accatgttgt tattatcgct ccccgccgtg
    10981 gcaattggga ttgcgtccag tcttattctg attgtggtga tgaaaatcgc ctcggtatta
    11041 cagaatttgc tctggcaacg actgccggga actctgggga tagcccagga ttcacccctc
    11101 tggatcatcg gtgtattaac gctaacgggt attgcggtgg ggttggttat ccgtttcagc
    11161 cagggtcatg ccggaccaga ccccgcctgt gaaccgctga tcggcgcacc ggttccgccc
    11221 tctgcgctac ctagacttat cgtagcatta attctcggtc ttgctggcgg cgtcagcctg
    11281 gggccggaac atccgatcat gaccgtcaat atcgcccttg ccgtcgccat tggcgctcgt
    11341 ctgttaccgc gcgtcaaccg aatggagtgg actattttag cctctgccgg aaccatcggc
    11401 gcgctgtttg gtacacctgt tgcggcggcg ttgatatttt cgcaaacctt aaatggcagt
    11461 agtgaagttc cgctatggga tcgtcttttt gcgccgttaa tggcggcagc agctggtgca
    11521 cttactaccg gattattttt ccatccccat ttttcactgc ccattgcgca ttacggacag
    11581 atggaaatga ccgatattct cagcggtgca attgtcgcgg cgattgccat cgcagcaggg
    11641 atggtcgccg tatggtgctt accacggttg cacgcgatga tgaatcaaat gaaaaatccg
    11701 gtgctcgtgc tgggtattgg cggatttatt ctcggaattc tgggggttat tggtggacca
    11761 gtttcgctgt ttaaagggct ggatgagatg cagcagatgg tggcaaatca ggctttcagc
    11821 accagcgatt actttttgct ggcggtaatt aaacttgccg ccctggtcgt tgctgccgcc
    11881 agtggctttc gcggtgggcg aatcttcccg gcagtgtttg tcggcgtggc attagggttg
    11941 atgctgcatg agcacgttcc cgccgttcca gcggcaataa ccgtttcctg cgctattctc
    12001 ggcatcgtgc tggtggtaac acgcgatggc tggttaagtc tttttatggc ggcagtcgtt
    12061 gtacccaata ccacattgct accgctgctc tgtatcgtca tgcttccggc atggctgtta
    12121 ttagcaggta agccgatgat gatggtcaat cgtccgaagc aacagccacc ccacgataat
    12181 gtttagcaaa aaatgctctc ctttatgatt taagagttat ggcgatgata cggggcttta
    12241 cctccccgta atattgcgtt aacaggccgc tgacggcttc gatcgtgaag gagaataaca
    12301 atgttcaggt cactgtttct ggcggccgcc ctgatggcat ttaccccgct tgcagcaaat
    12361 gcaggtgaaa tcaccctact gccatcgatc aaattacaaa ttggcgatcg cgatcattac
    12421 ggtaattact gggacggtgg tcactggcga gatcgtgatt actggcatcg caattatgaa
    12481 tggcgcaaaa accgctggtg gcgtcatgat aatggctatc atcgtggctg ggataagcgt
    12541 aaagcatatg agcgtggcta tcgtgaaggc tggcgcgatc gtgacgatca tcgcggaaaa
    12601 ggccgcggac atgggcaccg ccattaaacg cgtcctccaa tggagcacaa tgcctgatgc
    12661 gacgctagcg cgtcttatca ggcctacaaa ccggctcttt aacgcgtagg gcggataagg
    12721 cgtttacgcc gcatccgcca atagtgccag atgcgacgct cattcaacta caatcccagc
    12781 gccgtcccca ccaacaacca gatattcagc gccacgacca gcactacaat cacccagcct
    12841 gtctgtttta cgcgtttgct gttcaccaga tcgcccatca acttgctgtc actggtgaaa
    12901 atcagcagtg gaaccagcgc cagggcgata ccaaaactta acagcacctg actcataacc
    12961 agaatccgtg tcggatctaa tcccatcaga atgacaataa atgacggcaa catggtgact
    13021 gtacgacgca cccacagcgg gatatggaag cgaatgaatc cctgcatcac cacctgcccc
    13081 gccagtgtcc ccaccaccgt tgaggacagt ccggcagcaa ccagacttaa cccaaagacc
    13141 gttgccgcag catggcttaa cagcggttgc agcgtcagat aagcctcatc aagatcggca
    13201 acaccagtat gaccagaaaa gtggaacgcc gccgcagctg tagccatcat caccagattg
    13261 acaaaaccgg caatcgtcat ggcgatagcc acatcccatt tggtggcgga ataacgttgt
    13321 tgacgcgaac cgccatgtaa atgctgagtg agcgaagagt gcaaataaat cacatgcggc
    13381 ataatcgtcg cccctaacac gcctgctgcc aggaagaccg cctccgaagt aggtaaactc
    13441 gggatcacca ttcctttacc cagctgcgcc aggttaggct gggagaaaat caactcgaca
    13501 atgtaagccg cggcaacaaa caacagtaac ccgccaatca ctttctccag cggtttttgc
    13561 ccgcgacgtt gcagcattaa aatcaggaaa gtcgcgatcc ccgtcagcac cgcgccctgc
    13621 aacaacgaaa caccaagaat gagtttaaaa ccgatcgccg caccaataaa ttccgccagg
    13681 tcggttgcca tcgcaataat ttctgcctga acccaataga accacactac gggacgcgga
    13741 tagtgatcac gaatctgctc cgccagattt ttaccggtgg caatccctag tttggcagag
    13801 aggatctgaa tcagcatcgc catcaggttg gcccaaacga caacccacag tagctgatag
    13861 ccgaagctag cacccgcctg aatattggtc gcaaagttac cgggatcgat ataaccaatc
    13921 gccgcaatga acgcaggtcc cattaatgcg agcctcatct tacgcgccgc ccgtccgctg
    13981 ctactctcaa cgcgatagtt cgtcatcttg tgcctctaaa acatagcctt tgctatgttt
    14041 catgctatgc taaacgagaa tgattatcaa attcatttaa atggattgtg gtgatttctc
    14101 tgatagacca agattatgat tacgaaaaga ttgatgactt gaatgttatg gttatgttta
    14161 atgttagcac atttacataa ctttcagctt ccatacacaa catagcagaa atgtatgaca
    14221 gatcactatt tttgaagcct gtcacaggac gtcattatag tgtgtgtcag atctcgtttt
    14281 ccttaaccat gttacataga atgtgcacgg aaatttaacc tgcctcatat ttggagcaaa
    14341 tatggaccgc gtccttcatt ttgtactggc acttgccgtt gttgtgattc tcgcactgct
    14401 ggtaagcagc gaccgcaaaa aaattcgtat ccgttatgtt attcaactgc ttgttatcga
    14461 agtgttactg gcgtggttct tcctgaactc cgacgttggt ttaggcttcg tgaaaggctt
    14521 ctccgaaatg ttcgaaaaac tgctcggatt tgccaacgaa gggactaact tcgtctttgg
    14581 tagcatgaat gatcaaggcc tggcattctt cttcctgaaa gtgctgtgcc caatcgtctt
    14641 tatctctgca ctgatcggta ttctccagca cattcgcgtg ttgccggtga ttatccgcgc
    14701 aattggtttc ctgctctcca aagtcaacgg tatgggcaaa ctggaatcct ttaacgccgt
    14761 cagctccctg attctgggtc agtcagaaaa ctttattgcc tataaagata tcctcggcaa
    14821 aatctcccgt aatcgtatgt acaccatggc tgccacggca atgtccaccg tgtcgatgtc
    14881 catcgttggt gcatacatga ccatgctgga accgaaatac gtcgttgctg cgctggtact
    14941 gaacatgttc agcaccttta tcgtgctgtc gctgatcaat ccttaccgtg ttgatgccag
    15001 tgaagaaaac atccagatgt ccaacctgca cgaaggtcag agcttcttcg aaatgctggg
    15061 tgaatacatt ctggcaggtt tcaaagttgc cattatcgtt gccgcgatgc tgatcggctt
    15121 tatcgccctg atcgccgcgc tgaacgcact gtttgccacc gttactggct ggtttggcta
    15181 cagcatctcc ttccagggca tcctgggcta catcttctat ccgattgcat gggtgatggg
    15241 tgttccttcc agtgaagcac tgcaagtggg cagtatcatg gcgaccaaac tggtttccaa
    15301 cgagttcgtt gcgatgatgg atctgcagaa aattgcttcc acgctctctc cgcgtgctga
    15361 aggcatcatc tctgtgttcc tagtttcctt cgctaacttc tcttcaatcg ggattatcgc
    15421 aggtgcagtt aaaggcctga atgaagagca aggtaacgtg gtttctcgct tcggtctgaa
    15481 actggtttac ggctctaccc tggtgagtgt gctgtctgcg tcaatcgcag cactggtgct
    15541 gtaaaaccat acataaaaaa gccggggatc attccccggc ttttttacgc ctgttaatca
    15601 gctaatggct gcgggcgacc aatcaaatac ccttgcagat attgcacccc gagcttatgc
    15661 aatagcgcct gctgctgttg cgtctcgaca aactccgcga ccacactcaa tgacttcgct
    15721 ttcgccagat cggtaattga tctcacaatc atcgcatcca gcgtattcgt gacaatatct
    15781 ttcacaaaga cgccatcaat tttgatgata tcagcctgca aacgctttaa ccgttcgtag
    15841 ttggcatatc cggtgccaaa gtcatcaatc gcaatccgga aaccaaactt atgcagctgc
    15901 tcgatgttgt acatgctgct ttctgcgtta gaaaacgcct gctcctcggt gatctcaaga
    15961 atgaccgcct gcggggagat gtgataacgc ttaaacagac gaataatccg cccggcaata
    16021 ttcttttgca gcagcgtgag cggcattaaa ttgactgaaa agcgcggtcc ttttttgtcg
    16081 caagggtgtg ttgccagcca cttcaacaag gattccagca cttgcaaatc aaaacgcgcg
    16141 ctaaggttaa actgagcaat aaggggcaga aacttatccg gggtcataat gccgccgtca
    16201 tatttcagtc gcgcgaggat ctcatcataa ccttcaccct ctttgttgcg aattggctgg
    16261 gcgtagagca gcaaatcacc ctgatctaac gccgtgcgaa tggtattcag caatagcacc
    16321 tgtttagtgg tctgcccgga aaccatctcc tctctgctat ccagcgccag cacatgatga
    16381 tgtgcgcagg attgctccgc cagccagctt aactgcccca acaagggttg cagggtttcc
    16441 tgatttccat caaaacgccc ccaggcagca ccatagccca tatccagccc ggtattgttc
    16501 cagtgaattt gccgactatt caggatgtta accatatgct ggagtcgccc ttccgtttcc
    16561 ggcccgctca gcactaacag cagttcacta cccggcaatt gatacaactt ttcgttttcc
    16621 tgcatcaacg gcagcagcgt acggcaaatt gagcggatac agtgaacgcg catcattaag
    16681 ccgtaatgac gactcataaa ctcaagatta tcaatgcgca ggcagcaaaa actcttgccc
    16741 gcctcttgct ccggcgcttg ttccaacgca cgaaagttgg gtaggagcgt taacggatct
    16801 gttagcgcct gcaaatgcca gcggcgatta agccattcac tgcgatgata aatgcgcacc
    16861 atatagagca ggcaaacgct aaaggaaatc agcaccgcga gaataaatgc cagcgaatat
    16921 tcggtttcca ccccttgcaa aaagttctga ttgtaattca gaaggcaaag cgttgaaaca
    16981 gcccaggtga gatttaaaaa cggatagcga agattaccga ccccgagggt aaagatgatg
    17041 aagaaaacgg gtaccaggta accggcaata aagtcgtttt cataaggtgt gcacaacaga
    17101 agtagcagca cgcttaacgc tgccagccag cttaaggtaa atgcgagtga gatagtagaa
    17161 aagcatgttg taaatcagca ctgcggtgaa aaggcttagt aaatcaacga ccgtgaaaat
    17221 ggcatccgca tcgccgaaaa aggtggatat ctttagcggg aaatcaaaga agctccccac
    17281 aagatacatg ctgcatttga tgccgatcgg cgtcaccaaa ccaagccaga acagacgttg
    17341 ccagacatat cggctggtca atccataacg ccaacgcgtc cccagctgcc agcgtaatat
    17401 tgcgcaggca cacaacacga caaacgtctg gctgccgagc atgaccgcag tctgtaaaaa
    17461 agagagatga aagttccaca gattggtgca aaacatcccg cataaaacgg gaacgacacc
    17521 gcgccagcca aagataaaca agattgacag cattacgcac agtggcatcc acgccagaaa
    17581 gatataactg gaatggataa tggcaagtgg cgaaataaaa cgggatagct gaataagtac
    17641 cacggtgagc gtaaacgcta gtgtgaatat cttgatattt tttatcaggt tatgctccac
    17701 aaacatgagt acactactct cactgatcgg ggttttcagg cacacaaata ataagtttat
    17761 tcgcatcgcg aagcaaacag gggccagcct gttgccgatt tttcatccaa tgatgctttc
    17821 accaggcggc gctaacccgg acgtagactg tattacaaaa gcggcaaaaa gcagagacaa
    17881 aaaacccccg ctttgcagcg agggttggaa atttggtgga gctaagcggg atcgaaccgc
    17941 tgacctcttg catgccatgc aagcgctctc ccagctgagc tatagcccca cgatgcgttt
    18001 acgtaccaaa tttgctgggt gcaaaatttg gtggagctaa gcgggatcga accgctgacc
    18061 tcttgcatgc catgcaagcg ctctcccagc tgagctatag ccccgtcacg taaagcttgt
    18121 cgagttgacg ggcggcatca tatgaattcc gcccgaatgt gtcaacggca aattgcaacg
    18181 tgtagtttca atcgctgaaa aatcaggcaa atgaacaatt ttggaaaacc gctcgcattc
    18241 agtagttatt catgtcacgg tttcctgtaa agtggtgtta taaaatgaac tactaataga
    18301 cccacataca ttcagggaat tgttatgttc aaggagagga tgacaccaga tgaacttgcc
    18361 agactgaccg gctatagccg ccagaccatt aataaatggg tacgcaagga aggctggaca
    18421 acgtcaccaa aacctggcgt ccagggtggc aaagcccgac tggttcacgt caatgaacag
    18481 gttcgtgaat atattcgcaa tgctgaacgt ccagaaggtc agggagaagc gcctgccctt
    18541 tctggtgatg cgccgcttga agtattgctg gtgacactgg cgaaagagat gacgccagtt
    18601 gaacaaaaac agtttacatc cttgcttctg cgggaaggga ttatcggatt gttacaacgc
    18661 ttagggattc gcgatagcaa ataatatgaa aagattacgc aataaaatga ccaccgaaga
    18721 actggctgaa tgcctcggtg tggctaaaca aactgttaac cgttggacca gagaaaaagg
    18781 ctggaaaacg gaaaaatttc ccggcgtgaa aggcggtcgt gccagactta ttctggtcga
    18841 tacgcaagtt tgcgagttta ttcagaacac gccagccttc cataacacac caatgttgat
    18901 ggaagctgaa gagagaattg ctgaatacgc tcctggagca cgcgccccgg cgtatcgaca
    18961 aatcatcaac gcgatagata atatgacaga cattgagcag gaaaaagtcg cgcaattttt
    19021 gtcacgtgaa ggaattcgca atttcctcgc ccgtctcgat attgacgaat cagcataaat
    19081 aaaaaacggc aggatattat ctcctgccgt ttatcttttt acacgctaat tactgctgat
    19141 tttcgcgttc agcaataaaa tccagcgctt tgttgatacg ctcgatactg cgggtcttac
    19201 caattgcgtg aacggtaaca tccagtgctg gagactgccc cgcaccggtt acggcgacac
    19261 gcagcggcat accaacttta cccataccca cttccagctc atccgccgtc gcctgaatag
    19321 cgtgatgaac gttttcagcg gtccagtcag taatcgcggc cagtttgtca cgaaccactt
    19381 ccagcggctg acgcgctacc ggacgcagat gttttttcgc cgcatcggca tcgaactcag
    19441 caaaatcttc gtagaaataa cggcagctct gtgccatctc tttcagcgtc ttgcagcgtt
    19501 cgcccagcag tttcaccaga tcagccagct gcgggccgtt acgggtatcg atattttcct
    19561 gctcaatgtg ccactgtaag tgagtagcaa catactccgg cggcagcgcg ttaatgtagt
    19621 gatggttcag ccacagcagc ttgtcggtgt tgaacgcact ggcagatttg ctgacggcat
    19681 tcaaagtgaa gtatttgatc atctcttcac gagtgaagat ttcctgatcg ccgtgggacc
    19741 agcccagacg caccagatag ttcagcagtg cttctggcaa ataaccgtca tcacgatact
    19801 gcattacgct gactgccccg tgacgtttgg acagtttttt accgtcatcg ccattgatca
    19861 tagaaacgtg cgcgtaaacc ggcaccggcg ctttcagggc cttaagaatg ttgatctggc
    19921 gcggcgtgtt gttgatatgg tcttcgccac ggataacgtg ggtgatttcc atatcccagt
    19981 catcgacaac cacacagaag ttataggttg gggaaccatc ggtacggcgg ataataagat
    20041 cgtccagttc ctggttgctg aactcgatcg gaccacggat ctgatcgtca aaaacaacag
    20101 aaccttcctg cgggttagca aaacgtacaa cacacggttc atcatcagca tgatgctcat
    20161 ggctgtggcg gcagcgaccg tcataacgcg gcttctcacc tttcgccatt tgctcttcgc
    20221 gcagcgcttc caggcgctct ttagagcaat agcatttata agcagtgccc tcttccagca
    20281 tctgatcgat caccgcgttg tagcgatcaa aacgtttggt ctggtagtac ggaccttcat
    20341 cccactccag gctcagccag ttcatgccat ccataatggc ttcgatagct tccggcgtgg
    20401 aacgctcaag atcggtgtct tcaatacgca gcacgaactc accgccgtgg ttacgtgcaa
    20461 aaagccagga gtaaagagca gtacgcgcgc cgccaacgtg cagatagcct gttgggcttg
    20521 gcgcgaagcg agttttgatt ttcatgaaat ggccttacgt ttagaaagat gccgacaacc
    20581 ggcaaatcct ggaaaaattg agtgggcgat attctatcac tcccgcctga ttcctcaatg
    20641 tagttcgggt ttctacctta cgcattattg ttttcgttga caaattgcgc aacacggtct
    20701 gttttgcgat catcttgctt aaatttacga cgaacgaaca atttctttag aaaatgcgtt
    20761 gactcatttt gaactctccc tataatgcga ctccacacag cgggggtgat tagctcagct
    20821 gggagagcac ctcccttaca aggagggggt cggcggttcg atcccgtcat cacccaccaa
    20881 ctactttatg tagtctccgc cgtgtagcaa gaaattgaga agtgggtgat tagctcagct
    20941 gggagagcac ctcccttaca aggagggggt cggcggttcg atcccgtcat cacccaccac
    21001 ttcgggtcgt tagctcagtt ggtagagcag ttgactttta atcaattggt cgcaggttcg
    21061 aatcctgcac gacccaccaa tgtaaaaaag cgccctaaag gcgctttttt gctatctgcg
    21121 atactcaaag attcgaacct gcagcaggtt tgagttgagc gcagcgaaac aacggagccg
    21181 ctcgcggcga cggcccgaag ggcgagcgaa gcgagtcatc ctgcacgacc caccaatgca
    21241 aaaaagcgcc ctaaaggcgc ttttttgcta tctgcgattt gtaagattga tttttcttct
    21301 caagttcctt gttcttctac cgatattacc tcgtacagtg acgaggactg aacaatggcc
    21361 actatctctt caacttcaat tccttccata caaacacaga gtagtaacag ggcatcacaa
    21421 ggtagcgatg ttgcttcaca aatcgcgcgg ataagtcagc aaatcatcaa actcactcaa
    21481 caaattaaag agattgtaga cactagcggc agtgctgaag acaagcaaaa acaggccgaa
    21541 ttgattcaac agcaaatcac cctactggaa acacaactgg cgcagttgca gaaacagcag
    21601 gcagaaaagg cgcaggagaa agagcagcgt ttgtctctga atgtatccct tctaaatcct
    21661 gttgaaaaaa cgactcatat cgatatctat atctgataca acggatcccc ttcccgacca
    21721 gccattagcg cattgccctg gtctcgtacc acgtcccata acgcttcagc ggcggtggaa
    21781 agtgaacggt tcttcctgcg caccagcatc aactggcgtt caaccaccgg cgtaatgcgc
    21841 ttaaccacca gtgggctacc ttcaggcaat ggtagcgcca gtgcaggaag aatactgatg
    21901 ccgatgcctg cggctaccat cgggaacagc gtcgccggat gtccaatctc ctgcacaata
    21961 ttcgcctgaa taccattgcg cgccagcgct gcgtcaatca gcggtcggct gcctgacgcg
    22021 taatcctgca acaccaattt cgctccctgt aatgcctgcc agggtacata atcttccacg
    22081 gccaaagcgc tatcgcgatg gcaaagcaga aagaaaggct cggaaagaat cgcttcacat
    22141 tgcagatcgc ccactgggcc tggatcgatg acgatgccaa agtcgacatc tccctggcga
    22201 atactttcca tcacccactg ctgcggacga tcatgcagga caaactgaat atctggatag
    22261 cggcgatggc tttcagcaat gcattgcgga ataagatgcg cggaaatggt ctggctggca
    22321 gcgacccgaa cttttccgct cagttgttgc cccatacgcc cggtatcgcg caacgtgctg
    22381 ttaagttcat ccagcagtcg ctcaagacgc aaagccaact gctgccctgc atctgtaagc
    22441 accacttcac gcgtggttct gtccagcagg cgaacaccag tatgattttc cagctccttc
    22501 acactgtgac ttactgccga ctggctcaag ccgatacgct ctcctgcacg actaaaactt
    22561 ttctcctgcg ctactgtgac gaaaaccttt aattgtttta aagaataatt catctgtttt
    22621 cttcatgaat ggatgcaata aatcaatttt atttctcaaa ctggaagaag cacaatagaa
    22681 ccatcgatca tctggagtct ttatgaaact ttttcgtatc ctcgatcctt tcaccttaac
    22741 cctgatcacg gtggtgttgc tggcctcttt ctttccggcc agaggcgatt tcgtcccctt
    22801 ctttgaaaat ctgaccaccg cagctattgc cctgctgttc tttatgcacg gcgcgaagtt
    22861 gtcgcgtgag gcgattattg ctggcggtgg tcactggcga ctgcatttgt gggtaatgtg
    22921 cagcaccttc gtgctgtttc cgattctggg tgtactgttt gcctggtgga aaccggtaaa
    22981 tgtcgacccg atgctctact ccggttttct ctacttgtgc attctcccgg ctaccgtgca
    23041 gtctgcaatc gccttcacgt caatggcggg cggtaacgtc gcggcggcgg tttgttctgc
    23101 gtcagcatcc agcctgctgg ggattttcct ttcaccattg ctggttggtc tggtgatgaa
    23161 tgttcacggt gcaaggggca gccttgagca ggtcggtaaa attatgctgc aactgctgct
    23221 gccgtttgtg ttggggcatc tttcccggcc gtggattggt gactgggtgt cgcgcaataa
    23281 aaaatggatt gcgaaaactg accagacgtc cattctgttg gtggtttata cagcgttcag
    23341 cgaagccgtc gttaatggta tctggcataa agttggctgg ggatcattgc tgtttatcgt
    23401 ggtggtcagc tgcgttcttc tggctatcgt gattgtagtt aacgtcttta tggcacgccg
    23461 actgagcttc aataaggcag atgaaattac tatcgtcttt tgtggttcga aaaagagtct
    23521 ggcaaatggc atcccgatgg caaacattct gttccccaca tcggtgatcg gtatgatggt
    23581 gctgcccctg atgattttcc atcagatcca attgatggtc tgtgcggtgc tggcgcgtcg
    23641 atacaaacgc cagaccgaac agttacaggc gcagcaggaa agcagcgccg ataaagctta
    23701 aagcggacgc ttcaggggct ggaccagttg cgtcagcccc tcggttttaa tcagcagcgt
    23761 gatttgcatt agctcaccga gcttccctgc cgggaactca tctttgcggg caaaccacag
    23821 cagatactcc tccggcaggt caattaagcg acgccctttg tatttgccaa acggcattat
    23881 cgtattggct atttcaatca gctgctcttt ttccatctca gctacccagc aaacgcagca
    23941 tttccgcttc gtcgatgact tcaatgccca gttcctgcgc cttcgccagt ttagatcctg
    24001 cagcttcacc cgctatcacc agatcggttt tcttcgacac gctgcccgcg actttcgcgc
    24061 ccagttcgac cagtcgagct ttagcgtcat cacgcgacat ctggcttaag ctgcccgtaa
    24121 gcaccacggt tttaccagca aacgggctgt caatctcttc cgcgttgata acgatcggcg
    24181 caggccagtg aacaccttcc gccaacagct cgctgatgac attgcggttg ctttcttcgg
    24241 caaagaagtt gtgaacgtgg gatgcaacga caatgccaac atcaggcacc ttttgcagct
    24301 cttcaatcga agcggcttcc agcgcttcca gcgtgccgaa atatgccgcc agacctgctg
    24361 cggtggcctc gccgacttca cggatgccaa gtgcatagag gaagcgagca aaggtggttt
    24421 ctttcgcttt ttccagcgcg ttaaccacgt tttgtgccaa ttttggcccc atacgctcca
    24481 gtccggtcag tttgcctgcg gtgagtttga acagatctgc cggagtatgg acatattctt
    24541 tttcaaccag ctgatcgatg attttgtcgc ccattccgtc aacatccatc gcacggcggg
    24601 aaacaaagtg tttcagcgac tctttacgct gcgcaccgca aatcaggcca ccggtacagc
    24661 gggcaaccgc ttcaccttcc acacgctcaa cgtcagaacc acataccgga caatgcgtcg
    24721 ggaatacaac ctcacgggta tcttccgggc gttcagaaag cacgacgtta accacctgcg
    24781 ggatcacgtc gccagcgcgg cgaatcacca ctttatcgcc aatgcgtaaa ccaagacgtt
    24841 cgatttcatc cgcattgtgt aaggttgcgt tactcaccag cacgccagca acatggacag
    24901 gttccagacg cgcaacaggc gtaatcgcgc cagtacgccc aacctgaaac tcgacgtcac
    24961 gcacaaaggt catctgctcc tgcgccggga atttaaacgc aaccgcccag cgcggggcac
    25021 gcgcgacaaa gccaagctgc tcctgctgtt ccagtgagtt gaccttaatc accacgccgt
    25081 cgatatcaaa gcccagcgtc gggcggtctt cttccacttt gtgatagaac gccagcactt
    25141 cttccgccga ttcacaaagc gttacccgat cgctgaccgg caccccccac tttttaaatt
    25201 gcagtaaacg gccaagatga gtatccggca gctcgccacc ttccagaaca ccaacgccat
    25261 agcagaaaaa agtgagcggt cgcttcgctg taatacgcgg atcaagctga cgcagtgaac
    25321 cagctgccgc attacgtggg ttagcaaaaa ctttcccgcc cgtgcgtcgc gcatcttcgt
    25381 taatcttttc gaaccccgcc tgcggcagga acacttcacc acgcacttcc agacgcgccg
    25441 ggatattctc tccgtgcagc ttcagcggaa tggcgcgaat agtacgcaca ttagacgtga
    25501 tatcttcccc ggtggtgcca tcgccacggg tcgcggcact gactaaaacg ccattttcat
    25561 acagaatact gacggcaagg ccatccagct tcagctcaca gcaccaggtg actttctcgt
    25621 tgtttttcag acggtcctgc acacgtttgt tgaaagcaag aaagctttct tcatcaaaaa
    25681 cgttatccag tgacagcatt ggtacttcat ggcgtatctg gctgaaagct gccagcggcg
    25741 cagcacctac acgttgggta ggcgaatcag gcgtaatcag ttctggatgt ttggtttcca
    25801 gctcgcgcag ttcgcgcatc agcctgtcgt attcagcgtc gggaatttcc ggcgcatcca
    25861 tcacatgata aagatattca tgatggcaaa gcgtcgttcg cagttctgtc agttgttgtt
    25921 cgattgattc catatcgcac catcaatgct aaaaaccccc gacaagcggg ggttcgaaga
    25981 ggagttaatt tgccttaagt gtatcaggcg ttggcgtctt tgacttcgcg gatgatgtcc
    26041 tggtactcgc gcaatttctg cggagtcatc atacggcgct gatcgtcaag cacgacaccg
    26101 cccacttcat cggcaatatg ctgcgcagat tgcagcatca gcttgaagtt ctgcagctcg
    26161 tcaccgtaag acggtacctg cataaagata gtgacacccg gagtagtgaa atccttcatt
    26221 tcaggatcaa aggttcccgg tttcaccata ttcgccaggc tgaataacgc cgggccgctg
    26281 ccatccgggc taagatgacg atggtaaata ttcatatcgc caaaaatgaa gcccgcttgt
    26341 tgaatgctgt taagaagcag ttcaccgttt agctcgctac cgtgatgcgc cgcgacgttc
    26401 atgataatca ccgcttcttt gcgcttcggt ttatccataa ctggagccgg ttccgctaca
    26461 ggctcaggct gtggtgccgc tacgggttct gcaggctgga aagcctgttg tgccggttgc
    26521 ggtgctgaat gcacaggctg cggcgctggc gcgacctgtg gcgaaactgg ctgctgcaac
    26581 ggctgttcag gctgcggctg ataggcaggc tgctgcaccg gctgcgctgg acgcggagca
    26641 tgttgcggcg gtacctgcgc ttcaggcggc tgctggaccg gttgacgcgg ctgcgcagac
    26701 gcataaggcg gtaggtactg gtgttgcggc gacggacgag cagcctcatg ctcctgagcg
    26761 ttagccgggg catgattcac gcggtgaaca cgaacctcac caacgccctc atcatcttcg
    26821 acatcctcgt cataagaatc gtcgtcacgt tttgacttca ttcgttttaa tggccgatcg
    26881 cggaacatag aagatcgttc tttacggctg gtccagaaac catgtaccag taaagcgatt
    26941 atggcgatcg cgccaacaat gattaatatc agacgcaaat cctgcatcat tatattctct
    27001 gttgttctaa caccttgcca ccacggcaaa catttactca ctaagagtat ttgccgatta
    27061 cctcaagtgc aagtgcacta ttaactttca cagcacaaag atagatgaaa tcgtgctttt
    27121 tgctgttttt tcgaacatat cctaactgtc cattgcgcaa ttacccggta aaatacgcag
    27181 aattttcctg ggattggtca aaaggagctc atcctgatta tggtttcatc attcacatct
    27241 gccccacgca gcggttttta ctattttgcg caaggctgga agctcgtctc gcaacctggg
    27301 attcggcgtt tcgttatttt accgctgctg gtcaatattt tgttgatggg gggcgcattc
    27361 tggtggctct ttacacagct cgatgtctgg atcccgactt tcatgagtta cgttccggac
    27421 tggctacaat ggctgagtta tctgttgtgg cctctggctg tcatctctgt gctgttagtg
    27481 tttggctatt tcttctccac gattgctaac tggattgccg ctccgtttaa cggtttattg
    27541 gctgaacaac tggaagcacg attgactggc gctacaccgc cagataccgg gattttcggt
    27601 atcatgaaag atgtgccgcg aatcatgaaa cgcgaatggc aaaaatttgt ctggtatctg
    27661 ccgcgcgcaa ttgtattact aattctttac ttcatccctg gtattgggca aaccgtcgcg
    27721 ccggtactgt ggttcctgtt tagcgcctgg atgttagcca tccagtattg cgattacccc
    27781 ttcgataacc acaaagtgcc gtttaaagag atgcgcaccg ccctgcgcac acgcaaaatc
    27841 accaatatgc agtttggtgc tttaaccagc ctgtttacga tgatcccgct gcttaatctg
    27901 ttcatcatgc ccgttgccgt ttgtggcgcg acggcgatgt gggtcgattg ctatcgcgat
    27961 aaacacgcga tgtggcggta acaatctacc ggttattttg taaaccgttt gtgtgaaaca
    28021 ggggtggctt atgccgcccc ttattccatc ttgcatgtca ttatttccct tctgtatata
    28081 gatatgctaa atccttactt ccgcatattc tctgagcggg tatgctacct gttgtatccc
    28141 aatttcatac agttaaggac aggccatgag taagattttt gaagataact cgctgactat
    28201 cggtcacacg ccgctggttc gcctgaatcg catcggtaac ggacgcattc tggcgaaggt
    28261 ggaatctcgt aaccccagct tcagcgttaa gtgccgtatc ggtgccaaca tgatttggga
    28321 tgccgaaaag cgcggcgtgc tgaaaccagg cgttgaactg gttgaaccga ccagcggtaa
    28381 taccgggatt gcactggcct atgtagctgc cgctcgcggt tacaaactca ccctgaccat
    28441 gccagaaacc atgagtattg aacgccgcaa gctgctgaaa gcgttaggtg caaacctggt
    28501 gctgacggaa ggtgctaaag gcatgaaagg cgcaatccaa aaagcagaag aaattgtcgc
    28561 cagcaatcca gagaaatacc tgctgctgca acaattcagc aatccggcaa accctgaaat
    28621 tcacgaaaaa accaccggtc cggagatctg ggaagatacc gacggtcagg ttgatgtatt
    28681 tattgctggc gttgggactg gcggtacgct gactggcgtc agccgctaca ttaaaggcac
    28741 caaaggcaag accgatctta tctctgtcgc cgttgagcca accgattctc cagttatcgc
    28801 ccaggtgctg gcaggtgaag agattaaacc tggcccgcat aaaattcagg gtattggcgc
    28861 tggttttatc ccggctaacc tcgatctcaa gctggtcgat aaagtcattg gcatcaccaa
    28921 tgaagaagcc atttctaccg cccgtcgtct aatggaagaa gaaggtattc ttgcaggtat
    28981 ctcttctgga gcagctgttg ccgcagcgtt gaaactacaa gaagatgaaa gctttaccaa
    29041 caagaatatt gtggttattc taccatcatc gggtgagcgt tatttaagca ccgcattgtt
    29101 tgccgatctc ttcactgaga aagaattgca acagtaatgc cagcttgtta aaaatgcgta
    29161 aaaaagcacc tttttaggtg cttttttgtg gcctgcttca aactttcgcc cctcctggca
    29221 ttgattcagc ctgtcggaac tggtatttaa ccagactaat tattttgatg cgcgaaatta
    29281 atcgttacag gaaaagccaa agctgaatcg attttatgat ttggttcaat tcttccttta
    29341 gcggcataat gtttaatgac gtacgaaacg tcagcggtca acacccgcca gcaatggact
    29401 gtattgcgct cttcgtgcgt cgcgtctgtt aaaaactggc gctaacaata caggctaaag
    29461 tcgaaccgcc aggctagact ttagttccac aacactaaac ctataagttg gggaaataca
    29521 atgttccagc aagaagttac cattaccgct ccgaacggtc tgcacacccg ccctgctgcc
    29581 cagtttgtaa aagaagctaa gggcttcact tctgaaatta ctgtgacttc caacggcaaa
    29641 agcgccagcg cgaaaagcct gtttaaactg cagactctgg gcctgactca aggtaccgtt
    29701 gtgactatct ccgcagaagg cgaagacgag cagaaagcgg ttgaacatct ggttaaactg
    29761 atggcggaac tcgagtaatt tcccgggttc ttttaaaaat cagtcacaag taaggtaggg
    29821 ttatgatttc aggcatttta gcatccccgg gtatcgcttt cggtaaagct ctgcttctga
    29881 aagaagacga aattgtcatt gaccggaaaa aaatttctgc cgaccaggtt gatcaggaag
    29941 ttgaacgttt tctgagcggt cgtgccaagg catcagccca gctggaaacg atcaaaacga
    30001 aagctggtga aacgttcggt gaagaaaaag aagccatctt tgaagggcat attatgctgc
    30061 tcgaagatga ggagctggag caggaaatca tagccctgat taaagataaa cacatgacag
    30121 ctgacgcagc tgctcatgaa gttatcgaag gtcaggcttc tgccctggaa gagctggatg
    30181 atgaatacct gaaagaacgt gcggctgacg tacgtgatat cggtaagcgc ctgctgcgca
    30241 acatcctggg cctgaagatt atcgacctga gcgccattca ggatgaagtc attctggttg
    30301 ccgctgacct gacgccgtcc gaaaccgcac agctgaacct gaagaaggtg ctgggtttca
    30361 tcaccgacgc gggtggccgt acttcccaca cctctatcat ggcgcgttct ctggaactgc
    30421 ctgctatcgt gggtaccggt agcgtcacct ctcaggtgaa aaatgacgac tatctgattc
    30481 tggatgccgt aaataatcag gtttacgtca atccaaccaa cgaagttatt gataaaatgc
    30541 gcgctgttca ggagcaagtg gcttctgaaa aagcagagct tgctaaactg aaagatctgc
    30601 cagctattac gctggacggt caccaggtag aagtatgcgc taacattggt acggttcgtg
    30661 acgttgaagg tgcagagcgt aacggcgctg aaggcgttgg tctgtatcgt actgagttcc
    30721 tgttcatgga ccgcgacgcg ctgcccactg aagaagaaca gtttgctgct tacaaagcag
    30781 tggctgaagc gtgtggctcg caggcggtta tcgttcgtac catggacatc ggcggcgaca
    30841 aagagctgcc atacatgaac ttcccgaaag aagagaaccc gttcctcggc tggcgcgcta
    30901 tccgtatcgc gatggatcgt aaagagatcc tgcgcgatca gctccgcgct atcctgcgtg
    30961 cctcggcttt cggtaaattg cgcattatgt tcccgatgat catctctgtt gaagaagtgc
    31021 gtgcactgcg caaagagatc gaaatctaca aacaggaact gcgcgacgaa ggtaaagcgt
    31081 ttgacgagtc aattgaaatc ggcgtaatgg tggaaacacc ggctgccgca acaattgcac
    31141 gtcatttagc caaagaagtt gatttcttta gtatcggcac caatgattta acgcagtaca
    31201 ctctggcagt tgaccgtggt aatgatatga tttcacacct ttaccagcca atgtcaccgt
    31261 ccgtgctgaa cttgatcaag caagttattg atgcttctca tgctgaaggc aaatggactg
    31321 gcatgtgtgg tgagcttgct ggcgatgaac gtgctacact tctgttgctg gggatgggtc
    31381 tggacgaatt ctctatgagc gccatttcta tcccgcgcat taagaagatt atccgtaaca
    31441 cgaacttcga agatgcgaag gtgttagcag agcaggctct tgctcaaccg acaacggacg
    31501 agttaatgac gctggttaac aagttcattg aagaaaaaac aatctgctaa tccacgagat
    31561 gcggcccaat ttactgctta ggagaagatc atgggtttgt tcgataaact gaaatctctg
    31621 gtttccgacg acaaaaagga taccggaact attgagatca ttgctccgct ctctggcgag
    31681 atcgtcaata tcgaagacgt gccggatgtc gtttttgcgg aaaaaatcgt tggtgatggt
    31741 attgctatca aaccaacggg taacaaaatg gtcgcgcctg tagacggtac cattggtaaa
    31801 atctttgaaa ccaatcacgc gttctctatc gaatctgata gcggcgttga actgttcgtc
    31861 cacttcggta tcgacaccgt tgaactgaaa ggcgaaggct tcaagcgtat tgctgaagaa
    31921 ggtcagcgcg tgaaagttgg cgatactgtc attgaatttg atctgccgct gctggaagag
    31981 aaagccaagt ctaccctgac tccggttgtt atctccaaca tggacgaaat caaagaactg
    32041 atcaaactgt ccggtagcgt aaccgtgggt gaaaccccgg ttatccgcat caagaagtaa
    32101 ttcttgccgc agtgaaaaat ggcgcccatc ggcgccattt tttatgcttc cgccagcggc
    32161 ggcaaaatca attcatcgct ctcatgctgc tgggtgtagc gcatcacttc cagtacgcgc
    32221 aaccccgcac ggtgcaccgc atcgtttaac gccttccctt tcagcaaacc actgataagc
    32281 tgagcacaaa acaggtcgcc agtccctttc aggtcggttt ttacccgtga atgggaaatg
    32341 acattcacgc tgtcggcact gaccaccacc acctgcatct cctgattttc ttcattaccg
    32401 gaggcgctgg taatcaccac ccattttaat gtgtctgaaa gcagactttt tgcggcagca
    32461 atggcactgt cgagatcgcg gcaattttta ccggtcagga tttccagctc aaagatattg
    32521 ggggtaattc cctgcgccag cggcagtaaa tattgtcgat acgcctcggg aaggtcaggt
    32581 ttgacataaa ttccgctatc aatatcgcca atcaccggat cgaccatgat caataggtca
    32641 ggatggtctt tgcgtagcgc agtcagccac tcggcaagga ttttgatttg cgatgccgtt
    32701 cccatatagc ccgtggttac agcacgaagt tggcgcagcg catcacgctc ctgaagcgca
    32761 cgcaaatagc cgctaaacca ttcgtccgga atcgcaccac cgtagaaagt gtcataatgc
    32821 ggcgtattgc tcagcaatac cgtcggcacg gcaaagacat tcaagccgtt ctgttttata
    32881 gcaggcaccg caatgctgtt gcctacgctt ccgtacacta cctgcgactg cacggcgacg
    32941 atatccgcct gcagcgccct actcttatcg ttaaacaaca acaaactact catttaattt
    33001 tttctccttg ccgatgatcc tcatcgtatt ccaaccgaaa ctttacctga ttctggcagt
    33061 caaatcggct attacaaaac aaagataagg taattcagtg aagaaaatca tttgtctggc
    33121 cattacgctg ctaatgacac tccccgctta cgcgaagtta actgttcatg aagaagcccg
    33181 catcaacgcc atgctggagg gattagcaca gaaaaaggat ttgatatttg tgcgcaacgg
    33241 tgatgaacat acctgcgatg aagcggtttc ccacctgcgt ctgaagctcg gcaatacccg
    33301 taaccgcatt gacactgccg agcagtttat tgataaggta gcttcgtcgt catcgattac
    33361 cgggaagccg tatatcgtga agatccccgg taagagcgat gagaacgcac agcctttttt
    33421 acatgcgtta attgcgcaga cggataaaac ggtgcctgcg gaaggaaatt aatccgcttt
    33481 ggtaaggcat ttacaggagg taacatgaaa aaatgcttta tttatcacga tgaaaaatcg
    33541 aataaatttt ggtggataga ttacgaaggg gatagtttag ctgtcaacta tggcaaggta
    33601 ggtagtattg gtaaattcca tacaaaagaa ttcgataatg aagaacagtg tctgaaagaa
    33661 gccagtaaat tgattgccgc aaaaatgaaa aaaggatatc aagaaactcc agactttaac
    33721 ttcatggatc tctactattt tgatgatgaa gagattgggt tacatcttaa aacgtcacac
    33781 ccaaacttcc agtgccattt tactgatcca ctttatatgt gttgctggga tgaagaatct
    33841 ccttttggta gcgatgaagg tgctgatgct ctaaacgaac ttgaaaatag cctccgttct
    33901 ggactgtgct gatttccctc aaatgttaat tgaaactatg tggggtatga aatacatcgc
    33961 tatggacagt attcttgaag aggatgtgcg tgcgcaatta ctagccgatg aaatgagctc
    34021 tatccagagc aatatgatta cctacgcgac tgcattcggt cagatcaaag tcatgggtaa
    34081 aatctcccat aaacttaaaa agatgggact caatgcacta gcgcgtcatc agcttaccgc
    34141 aaaaattctt caatggggtg acggtcagga ctcaccaata cttcaaaaaa tgattgatga
    34201 ccttactgct tttccacacg aaaattaaaa actgcatttg tcggtagcaa caactgccga
    34261 caaatcagat tattgacaaa gtgcactttg tttatgccgg atgcggcgta aacgcctgat
    34321 ccggcataaa aatattgcta attcaatata ttgcagaaac attgtaggcc tgataagcgg
    34381 agcgcatcag gcagttttgc gtttgccatc agtctccaat gctattaatc cttaaatccc
    34441 tgccccctga ctaaaatgct cttccccaaa cactccggtg gagaggtagc gatcgccacg
    34501 atcgcagatg atcgctacca ccaccgcacc agggttagct tttgccaccc gcagtgctcc
    34561 agcaaccgcg ccgcctgagc tgacgccaca gaatattcct tcccgcaccg ccagttcgcg
    34621 catggtgttt tccgcatcgc gctgatgaat atccagcacc tcatccacca gcgcagcgtt
    34681 gaaaatcccc ggcagatatt ccgcaggcca gcggcgaatg cccggaatac tgctgccttc
    34741 ttccggttgc aggccaacaa tggtcaccgg tttggattgt tcgcgcataa agcatgaaac
    34801 gccggtgata gtgccggtcg tccccatgct ggagacaaaa tgagtgatgc gcccgccggt
    34861 ttgctgccag atttccggcc cggtggtggt gtaatgcgca taagggttat cgggattatt
    34921 gaactgatcg agcagctttc cttcgccacg attcgccatc tccagcgcca gatcgcgcgc
    34981 accttccatg ccctgctctt tggtgacaag aatcagttcc gcaccataag cacgcatcgc
    35041 cgcacggcgt tcctggctca tgttgtcggg catcagcaat ttcatgcgat agcctttcag
    35101 cgcggcaatc attgccagcg caatgccggt gttaccactg gtggcttcga ttaagacatc
    35161 acccggttta atttccccgc gcttttccgc ctcgacgatc atcgaaagtg ccgcacgatc
    35221 tttcaccgaa cctgccgggt tattgccttc cagttttaac cacacttcac tgccgttatc
    35281 cggccccatt cgctgcaact tcaccagagg cgtattgcct attgtttgtt ctaatgtact
    35341 cacaatctct atccatacgt ggtgttgcct gatgcgacgc ttgcgcgtct tatcaggcct
    35401 acaggttaca aaccttgcca ttaaaaagcc cggatcgcgg gaagcgcctc cgggcgttta
    35461 acattcactc aacctatcag gcgctttgtg cgagagcaag ttcctcatcg cgggtttcaa
    35521 tacgctcgtc gccgttatac agccgcgcat gttgcagacc aacgaataaa cgctcgccat
    35581 gctgcggggc atcgtcgcca tgcatcacga ccgtcagcgg ttcgttgtac caccccagcg
    35641 gctgcaccac taattgggtg tagtgacctt tcgggctggc ttccagtacc tgtaccggca
    35701 gcggcgaatc gagactggta cggcggctga tatccacttc ccacgggcgc aggaagagat
    35761 ccaccggccc ctgatacgca ggtgtgtagc ccagcggcca gcgatgcgcg ccaacatgga
    35821 actgcccgcc gcgaatggtt ccctgcaggc ggttcacttc gcccataaat tcgagtacaa
    35881 aacgggtcgc tggttcgcgc catacctgat tcggcgcatc agcctgttca atattcccct
    35941 gactcatcac cactacacga tcggctactt cggtcgcctc ttcctggtcg tgggtcacaa
    36001 acacgctggt gaatttcagc tcttcatgca gttgacgcag ccagcgacgc agctctttac
    36061 gcacctgcgc atccagcgcg ccgaacggtt catcaagcag cagaatttgc ggttctacag
    36121 ccagcgcgcg cgccagcgcc acacgctgtt tctggccgcc ggaaagctgc gccggataac
    36181 gatccgccag atgcgcaagc tggaccattt ccagcaattt tgtcactttc gctttgatgg
    36241 ctgcggcatt cgggcgctcg cgacgcggca gcaccgtcag gccaaaagtg atattgtcga
    36301 ataccgtcat atggcggaac agcgcgtaat gctggaacac gaaaccgact ttacgatcgc
    36361 gcgcgtgcag gcgactcacg tcggtgccgt ggaagcgaat atgcccgctg gtttgatgct
    36421 ccagcccggc gataatccgc agcagcgtgg ttttcccgga accggacggc cccagcaacg
    36481 cgaccatctg accagaagga atatccagtg agatatcgtt cagcacctgg gtgcgaccaa
    36541 aagacttctt gatattggca atctcaatgc tcatgatgct cctcctgctg cgcgcgtttt
    36601 tcctgattct ccaggcgcca ctgcaacata ctctttaaaa acagggtgat aatcgccatc
    36661 aacgtcaaca gcgcggcggc ggtaaaggag ccgacggtgt tgtagtcctg ctccaacaat
    36721 tcaatctgta acggcagcga aagtgtttcg ccgcgaatcg agccggaaac caccgacact
    36781 gcaccaaact cgccaatcgc gcgggcgttg gtcagcacca cgccgtaaag cagcgcccag
    36841 cgaatattcg gcaatgtgac gcgacggaac atctgccagc cggacgcgcc aagcaaaatc
    36901 gccgcttcgt cttcctggct gccctggctt aacatcaccg gcaccagctc gcgcaccaca
    36961 aacggacacg tcacaaagat ggtgaccagc accattccgg gccaggagaa cataatttgc
    37021 agattatgtt catccagcca gccgccgagc gggccgttgg agccgtagaa cagcagatac
    37081 accagaccgg caaccaccgg cgatacagca aacggaatgt ccagtagcgt cagcagtaac
    37141 tggcgtcccg ggaagttaaa gcgcgtcacc agccaggcca gcagaatgcc gaacaccagg
    37201 tttaccggta cggcaatcag cgcgatcatc accgtcagcc agatggcgtg cagcatgtcc
    37261 ggatcggcca gattctgtaa aaccggcatc agccccttgc tgaatgcctg cacgaagatg
    37321 taaatcatcg gcaccagcag gatgaacgcc gaaaccagca tcccgatgcc aatcagaaac
    37381 catttgcccc agttaatcgg gcgcgcgtca taacgcttca attgggtaac ttccgccatt
    37441 aatgacctac cacacgccga ccaaagcgac tttgcagagt gttaattgag aacagcagca
    37501 gcagagatgc cgcgaggatc accgaagcaa tcgcgctcgc tgccgggtaa tcaaactcct
    37561 gtaagcgcac aaaaatcatc agcgacgtca cttccgtctt ccacgcgata tttccggcga
    37621 taaaaatcac cgcgccaaat tcaccaagac tacgggtaaa cgacagcgcc acgcccgcca
    37681 ccagcgccgg agaaagctcc ggcagcacca ctttgcagaa actctgccag cgcgttgcac
    37741 caagcgtttc cgccgcttct tcatattccg ggcctaactc ttccagcacc ggctgcacgg
    37801 tacgcaccac aaacggaatg ctggtaaagg ccatcgccac cgcaatacca agccaggtat
    37861 aggtgacttt gatatcaaac ttcgccagcc attcaccgta aaaaccgttt acggaaaaga
    37921 gcgaggccag cgttaaaccg gcgactgccg ttggcagcgc aaagggtaaa tccatcagcg
    37981 catcaagcag cgtgcggccc ggaaagcgat aacgtgttaa aatccacgcc atcagcagac
    38041 cgaaaacgcc gttaaaaatc gatgccacaa acgccgacag cagcgtcact ttgtaggccg
    38101 cgaccacctg cgggttggtg atcacctccc agtactgcgc ccagctcatc tcagaaagct
    38161 gcatcaccag cgcagagagc ggcagcagca atatcaggca cacaaacagc agactggtgc
    38221 caaggcttaa agtaaagccc ggcagcacgc gtcttgaaga aactgcaaac attacttacg
    38281 ccccgccgct aacagcttgt ctaactcgcc gccgctggtg aagtgggttt tcatcacttc
    38341 cggccatgag ccaaatttgt cttccacgcg gaacagttcg gtctgcggga atttatcttt
    38401 cagtttgtcc atgacttccg ggttattcac acggtagtaa tagtcggtga tgatggtctg
    38461 cgcctgtggg ctgtagagcc agttcaggta ggcttttgcg gctttttccg taccgttggc
    38521 ctgcacattt ttatcgaccc acgccaccgg gaattccgcc agaatattgg ttttcgggat
    38581 caccacttca aagccctgtg cttcatactg tttacggatg ttgttcactt ccgattcaaa
    38641 gctaatcagc acatcgccca agccacgctc ggcaaaagtg gtggttgcgc cacgaccgcc
    38701 ggtatcgaac acttcaacgt ttttcaggaa ctgggtcata aactgttcgg ttttggcttt
    38761 atcgccaccg tcagctttat ccgctgcgcc ccatgccgcc agataggtat aacgcgcgtt
    38821 acccgacgtt ttcgggttcg ggaaaatcag cttcacgtcg gagcgcacca ggtcgttcca
    38881 gtcgtggata ttcttcgggt tgcccttacg caccaggaag cccatggtgg agtagaacgg
    38941 cgagctatta ttcggcaggc gcgactgcca gtcagccggg atcagcttgc ctttgtcgtg
    39001 caggatttgt acgtcggtca cctggttata agtgacaacg tctgctttta agccctgcaa
    39061 aatcgccagc gcctgttttg atgacccggc atgagattgt tttatcgtca gtttgtcgcc
    39121 gccgttatct tttgcccatt gctgttcaaa cggcggattc agggcggcaa acagctcgcg
    39181 ggagacgtca taagaactgt tcagcagttc cgttgcctgt acatggcccg ccagcagcag
    39241 agaagcgacc agcgcgagtg agttcttttt cagtaagtta acggccattg cgcaccctta
    39301 taaatttaat gactttctaa tagccatcat atttataacg gacgtgaaag gagtaacggt
    39361 tttatatacc gtttggtgat ttggaagtta aaaagggaat aagaacttgg ttcaacaggc
    39421 gtaattgttg caggcagttt ggacacggac agcgcggaga aaccggagcg tacacatagt
    39481 acgtgaggat ttcgagcact gcccggggcc aaaatgacaa atgaaatagc ctggtgaact
    39541 tagttcaaga cgaaatcctc cccacacatg cagggaggaa acagaggtga atcagatacc
    39601 gacgctaacc gtctccggca gtgtgctgcc gccatcaatc acattctgtg taccggttaa
    39661 atagctggat tcatccgatg cgaggaaggc cgccagttcg ccgacttcca gcggatcggc
    39721 gaggcgacac agcgggattg cttttgccat ttcagtcagc accgattctg gatcttccgg
    39781 gttcgactgg cgggcaatgc tttccgccat tggcgtgcgg acgtatcccg ggcagatggc
    39841 gttaacgcga ataccggatt gcgcgtactc caccgccagc gatttagtca ggccaacaat
    39901 cgccgctttc gttaaggcat acgccgtttc gccaggatca gccaccatat caccagtgac
    39961 tgaagacatc atcacaatgc gaccatcttt gcgcgcaatc atctccggca gcaccgcctt
    40021 cgtgacgttc catacgcctt taatattgat atcaatatgg aaatcgcgat cttcatcgct
    40081 catatcgagg aaactgccca gacgacaaac gcctgcgtta ttcaccagga tatcaatgcg
    40141 cccttctttt tccttcgcgc gtttgatagc tgcggctacc gacgccgggt cacgcacatc
    40201 ggcgacaacc gccgtacagc gatgaccacg accacacagt tcgtccgcta gcttttcgat
    40261 ctcaggggag atatccagca agattaggtt cgcgccatga cgtgcaaaag ttctggcaat
    40321 tccttcgcca attccctgca atgcgcccgt aatcagtgct gtcttgcccg tgagtttacc
    40381 cattttaatg ctctccttgt taatacagag cgttacactc cattccctct gaaaatacag
    40441 gataaacaaa atattgctgc ggatcagctc actaattcag gaaaatctca atccagtcgc
    40501 tgctttatgc atcatttcag gcgttgtgtt agctcactgc tgcgctgaat cattttcaat
    40561 gacgccacat cattgatgtt gcacaagacc aacaaacagt aaatcggtaa cagaattttg
    40621 cgccgtgcgg gtagacatcg acgagctacg ccattcggtt tcaccggaaa cggtatcgag
    40681 cgtgaaatgc gccagccgcc gcagcggaga gtcagccaga gaagtaatgg caatcaccgt
    40741 tgccccctgc tttcgtgccg cttctgcgca caagacaatc tcttttttac tgccgctgta
    40801 agagatagcg atttgtacat cgcctttttt caacgcctgg gaaacggtgg cttgtacgtg
    40861 cgtgtcggct tcgcaggcca cgcgatagcc aattttcatc agtttgaacg acagatcgcg
    40921 ccccaccagc gccgatccgc ccaggcccgt tatctggata aacggtgctt tactgatgac
    40981 ctcaatgatt ttttgcagcc gcgcgtagtc gaacaacgca caggtttgct ccagcgccag
    41041 ctctttttcg cgattcagtt tgcgggcaat cacttccagc gaatcatcac tggtaatcga
    41101 actgtgcagg tgcagcgccg tggcattcgt tttctcccgg ctggcactgt attcgccaat
    41161 taacgccata cgcaattcag taaatccttg cgcgccgagc ttctgggcaa atttcacaat
    41221 gctcgactgg ctgataccga gctgtttcgc catctgccgt gaagagacgg atttcagctc
    41281 gctcactctg gcccgcagaa agtcagcaat tttttgctcg ttcccggtaa attcactttc
    41341 cgcattgcgt attttcgtca ggtacaacat cttccactct cctaggctgg cagcaaaaat
    41401 tccgcttaag ttcttatgac gctcttcaca ctctgcgagt gtagactcaa tgattccttt
    41461 aagtctgtca attcgggata tatgattcca tacatctgca attatttgat cgtaaatagt
    41521 aaggtcacca ccgatgcaac tagaaaagat gattactgaa ggctcgaaca ccgcctcggc
    41581 tgaaattgac cgcgtatcga cgctggaaat gtgccggatt atcaacgatg aagataaaac
    41641 cgtaccgcct gccgttgatc gcgtcctgcc ggatatcgcc gcggcgatcg atgttatcca
    41701 cgcccaggtc agcggcggcg gtcgtctgat ttatctcggt gcgggaacat ccggtcgtct
    41761 ggggattctg gatgccagcg aatgtccgcc cacctacggc gtgaaaccgg gtctggtggt
    41821 tggtttgatt gctggaggcg aatatgccat tcagcacgcg gtggaaggag ctgaagatag
    41881 ccgggaaggt ggtgttaacg atctgaaaaa tattaattta acggcacagg atgtggtggt
    41941 tggcattgct gccagcggtc gcacgccgta tgtgattgcc ggactggaat acgcacgcca
    42001 gctcggctgc cgcacagtgg gaatttcctg taatccgggg agcgccgttt caaccaccgc
    42061 tgagtttgcc attacaccga ttgtaggtgc cgaagttgtt accggttctt cgcggatgaa
    42121 agcaggtaca gcgcagaaac tggtgctcaa tatgctttcc accgggctga tgattaaatc
    42181 cggcaaagtg ttcggcaacc tgatggtcga tgtggtcgcc accaacgaaa aactgcatgt
    42241 gcgccaggtc aatatcgtta aaaacgccac cggatgtaat gccgaacaag cggaagcggc
    42301 gttaattgcc tgcgagcgca actgtaaaac ggccattgtg atggtgctga aaaatctcga
    42361 tgccgcagaa gctaaaaaac gcctggatca acacggcggc tttattcgtc aggttttaga
    42421 caaggaataa cccatggcca aagagatcag cagtgaactt ctgaacacca ttcttacccg
    42481 tgtcggcgga ccgggaaata tcgccagttg tggtaactgt atgacgcgcc tgcgtctggg
    42541 tgtacatgac agttcactgg ttgatcccaa tatcaaaacg ctggaaggcg tgaagggcgt
    42601 cattttgacc agcgaccagg tgcaggtcgt ttttgggcct ggtaaagcgc atcgtgccgc
    42661 aaaagcgatg agcgagctgc tgggcgatgc accggtacag gatgccgcag aaatcgccgc
    42721 ccagaacaaa cgtcagttaa aagccaaaca aacctccggc gtgcaacaat ttctcgccaa
    42781 attcgccacc atcttcacgc cgctgatccc cggttttatt gccgccggtc tgctgctggg
    42841 gatagcgacg ttaattgcca cggtgatgca cgttccggca gatgctcagg gcacgctacc
    42901 cgacgcgctg aattttatga aggtgttcag caaaggtttg ttcaccttcc tagtgattct
    42961 ggtgggctat aacgccgccc aggcatttgg cggcacgggc gtcaatggcg caattattgc
    43021 cgcactcttt ttgctcggtt ataaccccgc tgcaaccacc ggttactacg ccggttttca
    43081 cgatttcttt ggtctgccca tcgatccgcg cggcaatatt atcggcgtgc tgattgccgc
    43141 gtgggcctgc gcccgcattg aaggcatggt gcgccgcttt atgccggacg atctcgacat
    43201 gctgctgacc tcgttaatca ccctgctgat caccgctacg cttgcgtacc tgatcattat
    43261 gccgctgggt ggctggttat tcgaaggtat gtcatggctg tttatgcacc tgaacagtaa
    43321 tccgttcggt tgtgcggttt tagccgggct gttcctgatc gccgtggtat ttggcgtgca
    43381 tcagggcttt attcctgttt acctcgcgtt aatggacagc cagggattca acagcttatt
    43441 tcccatcctt tcaatggcag gcgcgggcca ggtgggcgcg gcgctggcac tctactggcg
    43501 ggcgcaaccg cacagtgcgc tacgcagtca ggtacgcggg gcgattattc ccggcctgct
    43561 gggcgttggt gaaccgctga tttacggtgt caccctgccc cgcatgaagc cgtttgttac
    43621 cgcctgttta ggcggcgcgg cgggcggttt gtttatcgga ttgatagcct ggtggggtct
    43681 gccgatgggc ttaaacagcg cctttgggcc gtctggtctg gtggcgctgc cgctgatgac
    43741 ttccgcacaa ggcatccttc cggcaatggc ggtttatgct ggcgggattc tggtggcatg
    43801 ggtttgcggg tttattttca ccacgctctt tggctgccgt aacgtcaatc tggactgata
    43861 ttatgaaacg gacaatgctc tatctttctc tgctggctgt tagttgtagc gtcagcgccg
    43921 caaaataccc tgttctgaca gaaggtagtt gatcaagtat atgcggcgtt aaagcagtaa
    43981 taaaaaggcc agtcagtaat gttactgcct ggcctttaat caatgaatca gaaacgctta
    44041 cagcgccatc aacttgtcca gcgacggtgc gaaataatag ccgccggtta ccggtttggt
    44101 gaaacgcaac atcgcatcac gtttgccatc ggtatcgcca aacatgctca gcagttgctg
    44161 ctcaatgtta tgcagacgcg cgcagtaggc gcagaagtac agaccgtgag tgccactggc
    44221 ggtgccgtac ggcaggctct ggcgaacaat cttcagccct ttgccatctt ctttcagatc
    44281 aacgcgggtg aggtgagagg tttccggacg ttcgtcgccg tcaatctctt cgttggcctc
    44341 tttggtgcgc ccgatcatca tctcctgatc gtgaacgctc atccggttga gctgcttcag
    44401 gttgtgctcc caacgctgca caaacacata gctgccgccg gaatccacgc cgtctttgat
    44461 aaccgccact tcgcggcgcg tctcttcacc cgccgggttt tctgtaccgt caacaaagcc
    44521 gctcaggtca cgctcttcca cccaacggaa gccgtggatc tcttctttca cttcaatgca
    44581 gtcaccaaag gcttccatcg ccgcctgagc gacagagaag tttacgtcgt gacgcagaga
    44641 aagaatgtgg atcaacacat cgaactgggt cgtcggcgca aggcctttac cgtagcccgg
    44701 aaaatctttc agctcttctg ccccaacgcc gccgctcaga gcgcgccagg tgttgttacc
    44761 aaaggcaacc accgcaccaa gatgcgcgtc cgggaatttc gcttcaaaag ttgccagttt
    44821 gtcggcaaat gttttactgg ccgcacgcag ggcgtcaact tcccctttca cgttggcttc
    44881 gatccaaatc gccgcgcggc aatgttctgg caaaatgcca ctctgaacct gagacattgt
    44941 tcctcctgaa aataagaatg ccacgcaatc cgtggcattg ttgagcgcta ttttacgtgt
    45001 tttttcgcga cggcggtttg ttcagacgca aattaacgcc gccagataat tttactgact
    45061 ttccagtttt tcagggtatc gtccgaaggc attaaccctt caggaccacg ccattccccg
    45121 ctgaactggt agctaatatg ttgactgcct tcggctttac attcgacggc acgattatca
    45181 tcaccatcag ctttttggca attgccaaat gctttgctgt aaaggtcgct aaacggtgta
    45241 ccgattttaa caccggtgtc agcaggaata tcgctatcca gcacgtcaat gcgactgatc
    45301 gtgccttgat cgccgttaat caccatcgcc acgttgtcgc ctttcatcac ttcaaaaaag
    45361 cgcaccacgt tgccgttcgt ggttttcatt ccgctgcgca ggcgataatc gccatcaagc
    45421 gcatcagcaa tggcttgttc ttgcagtggt gtggacgccg ttaattcacc cacgccctgc
    45481 tcgctcactt tggtggacga tccaaaccag ttccacgggt tagcggcaga ccagttaact
    45541 gacgacatcg tggaacagcc ggtcagcatc aacggcatag cgcataacat taaacgcagc
    45601 gatttcatgt cacttccttt ggttattcaa taacgttgct tggagtgcaa attcaccaaa
    45661 aagtgccgtt attctttttc ttcattaaaa caagcgcgta aacgtcgatt ggtcagcagc
    45721 cagattaacg ccacaatatc cgccactacc agcgccagac cgataccgct aacggattca
    45781 ccgttcagcc acagccacgg ttgccagcaa agtaaaacca cctgcgccaa cagcaacaga
    45841 aaatagagca catgccaggt gcgaggaaac gaagcccgcc gaccgctcaa caaaaacgcc
    45901 agcaccgccg gaataccggg aatcagcccc agccagaaat tatcgtgatc gggataaaac
    45961 agatttagca gcgcagtacc ctgctcacgc gacgcaccgg caatgacaaa cagcacccag
    46021 gttcgcgcct gaagcaatag cacaagccag aagagcaagg gtaaacgcag gcgtccgtgc
    46081 gcatcataat ggacaggatg gaactcagta ctcttcatct tcaatcaaac gcttacccag
    46141 actcagcacg tcggcgtgtt catatcccag gcgttcatac attccgagca ccatgtcgtt
    46201 atcttccggc acgttgatct gaattttcgg gcagccgcga gcaatcagct ttttctccag
    46261 ccggttaagc aacgcattgg caattccacg cccacgaaac tctggatgca cgccaagata
    46321 ataagcagac ccgcgatgcc cgtcataacc gcccatcacc gttccgacca cttcaccgtt
    46381 tacctcagcg accaaaaaca aactgacgtc atggttcatc ttacgctcga tgtccatttc
    46441 cggatcgttc cacggacgca gcaagtcgca acgctcccaa agggtgatga cctcttcgaa
    46501 atcttcctgg cgaaatacgc gtatctccat ggtattcgtt acctttttgc gggttaaaag
    46561 gctgattatg gcgtgaacgg tcgaattagc caatatctga cgaaaatcgg ttgaaaaagt
    46621 ggcataatgg ggagttgtca actattgaaa tgaaaagtaa aacaattctc aacagcaaac
    46681 cgtcgtaacg gattacgcga tacgatataa catctgaaac tttattatta caactcaggc
    46741 cgtatgagca cttttaaacc actaaaaaca ctcacttcgc gccgccaggt gctgaaagcc
    46801 ggtttagctg ccctgacgtt gtcaggaatg tcgcaagcca ttgccaaaga cgaaccttta
    46861 aaaaccagca acggacacag caagccgaaa gccaaaaaat ctggcggcaa acgtgtcgtt
    46921 gttctcgatc caggccacgg cggaattgat accggagcga tcggacgcaa cggttcgaaa
    46981 gaaaaacatg tggtgctggc gattgctaaa aacgtccgtt ccattttgcg taatcatggg
    47041 attgatgcgc gtttaacgcg ttctggcgat acgtttatcc cactttacga tcgcgttgaa
    47101 atcgcccata aacatggcgc agatctgttt atgtcaattc atgccgatgg ctttaccaac
    47161 ccgaaagctg ccggtgcttc ggtatttgcc ctctctaacc gtggggcaag tagcgcaatg
    47221 gcgaaatacc tgtctgaacg cgaaaaccgc gccgatgaag ttgccggtaa aaaggcgact
    47281 gacaaggatc acctattgca gcaagtactg tttgatctgg tgcaaaccga tactattaaa
    47341 aacagcctga cgcttggctc gcatattctg aaaaagatta agccggtgca taaactgcac
    47401 agccgcaaca ccgaacaagc cgcgtttgtg gtgttgaaat cgccgtcggt tccttcggtg
    47461 ctggtggaaa cctcgtttat caccaacccg gaagaagagc ggctgttagg cacggcggcg
    47521 tttcgtcaga aaatcgccac agcgattgct gaaggcgtga tcagttattt ccactggttc
    47581 gacaaccaga aagcacattc gaaaaagcga taagttatga aacccgacgc acaccaggtt
    47641 aaacagtttc tgctcaacct tcaggatacg atttgtcagc agctgaccgc cgtcgatggc
    47701 gcagaatttg tcgaagatag ttggcagcgc gaagctggcg gcggcgggcg cagtcgggtg
    47761 ttgcgtaatg gtggtgtttt cgaacaggcg ggcgtcaact tttctcacgt ccacggtgag
    47821 gcgatgcctg cttccgccac cgctcatcgc ccggaacttg ccgggcgcag tttcgaggcg
    47881 atgggcgttt cactggtagt gcatccgcat aacccgtatg ttcccaccag ccacgcgaat
    47941 gtgcgttttt ttattgccga aaaaccgggt gcagagcccg tttggtggtt tggcggcggc
    48001 ttcgatttaa cccctttcta tggttttgaa gaagacgcca ttcactggca ccgcaccgcc
    48061 cgtgacctgt gccagccatt tggtgaagac gtttatcccc gttacaaaaa gtggtgcgac
    48121 gattacttct acctcaaaca tcgcaacgaa cagcgcggca ttggcgggct gttctttgat
    48181 gatctgaaca cgccagattt cgaccactgt tttgccttta tgcaggcggt aggcaaaggc
    48241 tacaccgacg cttatttacc aattgtagag cgacgtaaag cgatggccta cggcgagcgc
    48301 gagcgcaatt ttcagctcta ccgtcgcggt cgttatgtcg agttcaatct ggtctgggat
    48361 cgcggcacgc tgtttggcct gcaaactggc gggcgcaccg agtcgatcct gatgtcaatg
    48421 ccgccactgg tacgctggga gtatgattat cagccaaaag atggcagccc agaagcggcg
    48481 ttaagtgagt ttattaaggt cagggattgg gtgtaactcc ctcaccccca ttcccgcatc
    48541 cgctgatgca acgtcaacga cggcttctcg gcaaacagct gctggtaatc cgtggcaaat
    48601 tgccccagat gccagaatcc ccactgcatg gcggcgtctt ttaccgttgt gctttgcgac
    48661 cacgggctta tcagttcgcg gcgtacgacg ttcaggcgaa tgcgtttcag ccacgcgttt
    48721 gggccaatgc ctaaaatagc gtgaaacgcg ttttgtagcg tgcggcgact gacatgcagt
    48781 tgattacaca aatccagcac cgtcaccggt tcggacatgt tttccagcac atattcacgg
    48841 gcgcgggaaa gcaaccgacg gtaactctga tgactgatgc tttccgccgt caccattggt
    48901 tgcgcttctt ccagcatcgc ccccatcgcc attagcaaat tatcccccag cacttttcgc
    48961 actgcaggct gatggagatt ttccgggttc tcgcaaaacg tcgccagcgc ctgttggaca
    49021 aagccccaca gcgcggcttt atgctgctct ttcacttcca gcgccgactg gctacgcagc
    49081 atatgtaata cccgatccgg attgtgcaaa aagttagcct gacaggtgat gacatcttca
    49141 gacagcacta cgcccaggat cgtataatcg tccggcgtac ttaattcaaa ctcggttcca
    49201 ccggggcgcg tcgctatttc tgcgcttccc agacattgcg aaccgataaa tccctgctca
    49261 ccgcgcgtcg ccggaatgcc aaaccagaac gagttcggcc agaccaggca cgactgacgc
    49321 agcgccagac cggtgtattc acgaaaaacc tgaatatcat cgagtagaat ttccgtaaat
    49381 tcaccatgaa acttgcccgg atgcagctga tcgtaaatct gctgctaggc ggtaatcgtc
    49441 aaagcatgtt catagacatc cgttgtctgt cgttgatgaa cattatccac ttcgaccttc
    49501 ggcgtgagct tcaggttttc gggtaagggt tcatgataaa gatggtgcaa attggctgta
    49561 cgggtctttt tcatgatgtt aatgccgggt gttgtaggac acccggcacc tccgacaggt
    49621 taatggggct tgagacgata acgactactg cgtttacgta acgtcccggc agaaaagagc
    49681 tgttcgagcg catttctggc tttttccagc ggccagccaa agtgggcggc cacttctcct
    49741 gccgtcattc cctgacatac tgatgttaac agcgccaaca actcgtcagc agattccgcg
    49801 acgataactg gcgcgtcggg ttcctttacg ggttgcttcg gctggcggtt aaagccagta
    49861 accagccact gggtgtcatc gtctggccga ccgatctcct tacggctgat gacacgcccg
    49921 gtacgcatcg cggcagcaca acctgcgtcc agcgccgcac gacacgccgc cagatcgcct
    49981 tccaccacca gcgttaagcg accagggtca agcacttcgt gactgagcag acgaacgtta
    50041 gctgctttga gcatggcatc cgctgcatct atcgcggcga ccatgccgtc cacttccagc
    50101 aatcccagtg cattgatcat cggcaacctc cgttacgcac gctggattgg attacgcgcg
    50161 atttccagca ctgcatcggt aaaggcgtta caggctgctt tacacgcggc ctggctaccg
    50221 gttaaaaatg ctgccgagta gttggtttca gacggtggcg ggacgtaggt caccagctgt
    50281 acgtcggcag atttcaacgc ggcatcaatg ccgtaggtcg cttccagcgg cggtgccacc
    50341 agatacgcca tcggatcgcc aagcgtgatc ccggcggttg atgaaagata agaaccggta
    50401 cgcgaaacca catgcgccag gaaggcggtg ttttctgcgt cattggccca ctggaaagct
    50461 gcgccatttt caatatgtgc aaccatcgca tccagaccgg cacgcacttc cgccgggttt
    50521 ggcccaccaa gcataatcag cacttcaccg gcagtcggtg acgggccgtg tgccgctccc
    50581 gcatacagcg agcgaccata taccacttcg accatcgctt gtttggtcgc ttcgtcagcc
    50641 gcaatgtagg ttacgtcgtc agaatcggca gaaatcagcc cgaggctacg aatatgcggc
    50701 ggcaatttta gttcacgcgc aaattcagcg ttaacggagg caatcacccg catggcggtt
    50761 accgacggtc gaatcaaatc taaagctggc atgatgcctc cttatcgggt catgttgatg
    50821 ccggatgctt tctgctccag catacgtttc gccaaatcca caatgacggc acagcagcac
    50881 gaaagagaag caaaagtcag ctacctggta cactaggcag cgggtcagca aaggggggca
    50941 aatgcccctt ttttcatgtc agataatcag atcattacct caataaacta ataatcacat
    51001 cgcggttaca accagtttga tagtgatagc tcaaacccag acgtggcgct ggctgcaagc
    51061 aaaataagcc gcaaaaatgc gaccccatgt ttactagccg caaaaatgcg gtcctatttg
    51121 ccagaacctt ttattattca attggttagt gcaagaaaaa gcgtgattac tgttcaagaa
    51181 gtatccagaa aggtcatgat tttgacgctg aaaaaaaata ggtgggcatt tttgcccact
    51241 cactagccgc aaatttgccc actcactagc cgcatttttg cggcccaaag taataaatac
    51301 agagaaataa taaatacaga tcagcgccaa gatttttcgt cttttcggcg gaatcatgag
    51361 actagtgctt acgtcctgat aacctccaga gcagacctta acttctgtgc agcaggacta
    51421 cgctcattgc cgctcggcta ggtcaaatcc tcacgataaa gctgttcgga ttgtgaccat
    51481 aatgttaaag gactctttaa atcattcctt gacgaattaa ctagctggct taagcggaat
    51541 agcatgcagg ataaaaacaa aaccagctta gcagtgggga gagcttcgct tcgctcgtct
    51601 cctggggcca atgctcaatg aaacgacgta aggcacggag cgctcaccat aggtagacac
    51661 ataaacatct gagagcgcac cttctatcgc gctacgcgct caataaaaac caacgacacc
    51721 gccacactgc aagaccaatg gtttttatcg cgtatacaga ccagacagga aacagcagca
    51781 aagcagcgta tagccaggtg cagacagcac actcacacga cgcacagcag gcttaagtga
    51841 tcactcagga aactttatcc ccgccgccga tcaccgcgaa ctatcatgac cgcgccaaat
    51901 tcgaaaggaa tggcccatag aacgcgatga acacacataa gcgctacact tacttaacgt
    51961 cgccacagag cgattgagag cgcttaaaat cgatgcgatt tcattaaatt tttctcactc
    52021 gcctgaatgg tggcgatcag gactgatcgc aacattgccc cgcctcgata ttgagcgcat
    52081 aatagataca tacattaagc ggaaaagaga taagtttatt gtgaaaatca gggggttaca
    52141 tatcatgcgc atatcagaac aacaacggca gatcctcgac tatctggcaa aaaacgaatc
    52201 aatcaccgca cgacaagccg cagatctcgt ttatggtggc aatgttaccc gcgtccaggt
    52261 tgacgccgcc cgccgctcac tcatgacgat ggttagtaat gggctaatca ggaaacaagg
    52321 ccgcgctttc gtagctgaaa gacatatcaa tcacgcttat agccaggacg ttgaagaatt
    52381 gcgccgccag atcctttggg tattcagcaa acagggtcag gagtatttcc tttctgaaca
    52441 cccaaatttc gccgccaaag agttaacagt aagcggattg tcgtgcgcac tggattatct
    52501 tgtcggtgaa ggttgcgtta cttacaagca gctatggcac gccctgaata cgcttgtaga
    52561 agatggttta ctcattgcca cgcgtgagaa catcgaatac tggataatgc cgcgttcagg
    52621 tcgccgcaat catgcacggt atatttcagg caacagatac agaacgccgg actttaatcc
    52681 gacacaggaa gatcgggacc gtgccgaaat gatgtgcagg gaacaggaga aaagaattat
    52741 ggataagtta ttttcgatgc agtgaacaca agcgggcgca atgcccgttt ttattgcctt
    52801 attccacgcc tgacgaatca atttgatcac acctgagaaa atgcatagcc agaagttaag
    52861 gccgttatat tgcgattggg agcgttttaa acggtatcaa tttaattaat tctgatgtgg
    52921 tgatttgcag gcaaaaaaaa cccgccggag aagcgggaaa tagagacata tcgttgtagt
    52981 tattaacaga tcactttgag gtcgagggcg gctaaccctc gctaatatta tcccgctatt
    53041 accttgcggg tgcaaataat cttttattgc tttgatgatt taataaatag cactttttgc
    53101 taacaaaaac cgctgtcaat atatggccaa aaatgtcaac agaaaaaata aggtttataa
    53161 tattaagtat cacatgggta atgggttcag ggaatcggaa tccaccgcct cacggaaatt
    53221 caaaatttgc ttcttctttc ctccgtgggc ggcttctttt tcgtatcgag gtatttatga
    53281 aagcaataac attagaacag ttcgcagcaa tggcgaatgt atcgaccaga acgctgaggc
    53341 gctggctaaa aaaaccatca accggagtcc ctttgccatt taccccacgc ggtactcgcc
    53401 aattattctg ggagcacgaa tgcaaagaat ggttgtacag aacagaacga taataatcac
    53461 gcatcacatt ttgaggtaaa catcatgacc aatatcgaaa gattagaacg ctgtgccgat
    53521 attatgcgac gcagatggat ttatgaccct gaagcgggtt tattgtttag ccgtgaaaca
    53581 aaagacgtta tcaaaggcag catgaccaat aaagggcatt tggttgtaac ggtacatgaa
    53641 ggtaaattca aagtgacact cacctatcag aaagcctgct atgtatacac atacggcgct
    53701 tatgacgaga cattatatga agtgcaccac gttaatttga acaaacagga taaccgcatc
    53761 aagaatattc gcctgatgcc gaaagaaaag caccgccgga tacataaaaa cgtccgcaaa
    53821 cttattcggc tgggccacac acaatggctt caaccgcaat taattgaaca atcactataa
    53881 acacaggagt aaataccatg cattcattag acagttattt tcagcgcacg acagccccta
    53941 aatcagccgc ccaggaacgc cgtgaggaat tccatgagaa agtgatgcgt tctgctgatt
    54001 atattgcgga caagttcgtg gaaacggttc gtccacttgt tgatgaagta gctgataaac
    54061 ttcagtctga aatgccggaa gatatggaag gcactgcaaa gcggcgactc atttgcgaat
    54121 taagtcgtcg ttttggcgtg tcaataagcg cttttaaata atcgaaaaaa ggggggaaag
    54181 ataatggacg catttgaggt actggcaaat aaaggcgctg aactggtagc acagcgtgat
    54241 aaaaccgcca acgaagggga acgcgcactg ctaaataaac agattaaagc gatccgtatg
    54301 gcccagttta aattaatcag taatgaagtt atcgaaacaa ttaaaccgca gatcccacaa
    54361 atcgttgctg acgctattaa agccgccgga ctggagaaac gcattgccgg gctaaaaacg
    54421 aacgcagaga aaatggagat gtacaaacag agcggtgctc cgaatcccga tgagtacttc
    54481 tcaccggatg ttgagtttat ggcgcaggtt gaggaacgtc tgggtagctg tctcaccgag
    54541 gaacagcgtc gctattttga tggagtggat agttcggcag gtattgacct gaactcgtat
    54601 ttcggtcgtg aaattgagca ttttgatgcc gccccgtcta tgccggagcc agaaccagaa
    54661 atggccaccg atcctgaagc tgagcaacgg aaacgcgtat tcaatgcgat agggattcgg
    54721 tactgatcgg ataaaagaaa gccgggaatt accccggctg acttaacaat gtgaccaatg
    54781 aacaaatcgg acaagacaaa caggataagc agtctcgaaa caatgttcgg gaactatatt
    54841 agcacaagtc cggagaaaat catcatgttg gcactacgca tcatgcaggg catcgctaag
    54901 accctcgcag aacatgtctt agacctgaaa cattcgccac tgagcaaaca ggccatgaag
    54961 cgccaaacgc ttcgtctgtg ggctgaatat tcgcttggaa ctatcaataa aatcatcgac
    55021 atgaaatcgg ggccgagcaa ccagagcgcg gaggaaatgg agttcatcag gcgtttaatt
    55081 ctcattcgca gagacattca tagccagtta cacagcgtgg gaatcgatat taatgatggg
    55141 acgggcgatt aattttcgga caagtactat gcgcgcgcga ggagctgaac atgaagcaag
    55201 atattgcaga caggctggag atactcgaag gccagcgggc agaagcgaaa caactacgta
    55261 agcaggccag aagagcgcac aggaacaacg aagcagagtt actaaccaaa tacatcagtt
    55321 tcaccaatta ctgcatctac gagtgttaca aggaagatgc agaggactgg ctggattcat
    55381 tgccagaaca gtactgagca gaataacacc acgtcatgtt gttctgaggt gagcatatca
    55441 cctctgttca ggtggccaaa tttagtgtgc cacttcagca tcaccgttga aatcacaata
    55501 acgatttttt tgtaaatatg ctctcacata tgtaggaata gaccaggttg gcattgaaac
    55561 acatctacca tgaccataat aaagta
//
LOCUS       DAZPCS010000009        55418 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZPCS010000009 DAZPCS010000000
VERSION     DAZPCS010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55418
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.009"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(113..1486)
                     /gene="argH"
                     /locus_tag="ACHUQ7_000512"
     CDS             complement(113..1486)
                     /gene="argH"
                     /locus_tag="ACHUQ7_000512"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFR1134073.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            complement(1547..2323)
                     /gene="argB"
                     /locus_tag="ACHUQ7_000513"
     CDS             complement(1547..2323)
                     /gene="argB"
                     /locus_tag="ACHUQ7_000513"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFR1134074.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            complement(2331..3335)
                     /gene="argC"
                     /locus_tag="ACHUQ7_000514"
     CDS             complement(2331..3335)
                     /gene="argC"
                     /locus_tag="ACHUQ7_000514"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFR1134075.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            3489..4640
                     /gene="argE"
                     /locus_tag="ACHUQ7_000515"
     CDS             3489..4640
                     /gene="argE"
                     /locus_tag="ACHUQ7_000515"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFR1134076.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGINAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPQPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            4992..7643
                     /gene="ppc"
                     /locus_tag="ACHUQ7_000516"
     CDS             4992..7643
                     /gene="ppc"
                     /locus_tag="ACHUQ7_000516"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFR1134077.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYERNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            7826..9559
                     /locus_tag="ACHUQ7_000517"
     CDS             7826..9559
                     /locus_tag="ACHUQ7_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFR1134078.1"
                     /translation="MHSTEVQAKPLSSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            9708..10559
                     /locus_tag="ACHUQ7_000518"
     CDS             9708..10559
                     /locus_tag="ACHUQ7_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR1134079.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTEHSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10546..10887)
                     /locus_tag="ACHUQ7_000519"
     CDS             complement(10546..10887)
                     /locus_tag="ACHUQ7_000519"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFR1134080.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(10889..11767)
                     /locus_tag="ACHUQ7_000520"
     CDS             complement(10889..11767)
                     /locus_tag="ACHUQ7_000520"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFR1134081.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(11733..14030)
                     /locus_tag="ACHUQ7_000521"
     CDS             complement(11733..14030)
                     /locus_tag="ACHUQ7_000521"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFR1134082.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(14081..14401)
                     /locus_tag="ACHUQ7_000522"
     CDS             complement(14081..14401)
                     /locus_tag="ACHUQ7_000522"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFR1134083.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(14416..15495)
                     /locus_tag="ACHUQ7_000523"
     CDS             complement(14416..15495)
                     /locus_tag="ACHUQ7_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFR1134084.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            15804..18305
                     /gene="ptsP"
                     /locus_tag="ACHUQ7_000524"
                     /pseudo
     CDS             15804..18305
                     /gene="ptsP"
                     /locus_tag="ACHUQ7_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            18317..18979
                     /gene="fsa"
                     /locus_tag="ACHUQ7_000525"
                     /pseudo
     CDS             18317..18979
                     /gene="fsa"
                     /locus_tag="ACHUQ7_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            18990..20091
                     /gene="gldA"
                     /locus_tag="ACHUQ7_000526"
                     /pseudo
     CDS             18990..20091
                     /gene="gldA"
                     /locus_tag="ACHUQ7_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            20366..20973
                     /locus_tag="ACHUQ7_000527"
                     /pseudo
     CDS             20366..20973
                     /locus_tag="ACHUQ7_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
     gene            complement(21000..21905)
                     /gene="yijE"
                     /locus_tag="ACHUQ7_000528"
     CDS             complement(21000..21905)
                     /gene="yijE"
                     /locus_tag="ACHUQ7_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFR1134085.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(21999..24179)
                     /gene="katG"
                     /locus_tag="ACHUQ7_000529"
     CDS             complement(21999..24179)
                     /gene="katG"
                     /locus_tag="ACHUQ7_000529"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFR1134086.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPIQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(24508..25398)
                     /gene="metF"
                     /locus_tag="ACHUQ7_000530"
     CDS             complement(24508..25398)
                     /gene="metF"
                     /locus_tag="ACHUQ7_000530"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFR1134087.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            25474..25608
                     /locus_tag="ACHUQ7_000531"
     CDS             25474..25608
                     /locus_tag="ACHUQ7_000531"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1134088.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            complement(25747..28179)
                     /gene="metL"
                     /locus_tag="ACHUQ7_000532"
     CDS             complement(25747..28179)
                     /gene="metL"
                     /locus_tag="ACHUQ7_000532"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFR1134089.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDYPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(28182..29342)
                     /gene="metB"
                     /locus_tag="ACHUQ7_000533"
     CDS             complement(28182..29342)
                     /gene="metB"
                     /locus_tag="ACHUQ7_000533"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFR1134090.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            29619..29936
                     /gene="metJ"
                     /locus_tag="ACHUQ7_000534"
     CDS             29619..29936
                     /gene="metJ"
                     /locus_tag="ACHUQ7_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFR1134091.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            30120..30728
                     /locus_tag="ACHUQ7_000535"
     CDS             30120..30728
                     /locus_tag="ACHUQ7_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFR1134092.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(30789..31001)
                     /gene="rpmE"
                     /locus_tag="ACHUQ7_000536"
     CDS             complement(30789..31001)
                     /gene="rpmE"
                     /locus_tag="ACHUQ7_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFR1134093.1"
                     /translation="MKKDIHPKYEEITASCSCGNVIKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            31204..33402
                     /gene="priA"
                     /locus_tag="ACHUQ7_000537"
     CDS             31204..33402
                     /gene="priA"
                     /locus_tag="ACHUQ7_000537"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFR1134094.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDGEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            33558..34583
                     /gene="cytR"
                     /locus_tag="ACHUQ7_000538"
     CDS             33558..34583
                     /gene="cytR"
                     /locus_tag="ACHUQ7_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFR1134095.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            34675..35634
                     /gene="ftsN"
                     /locus_tag="ACHUQ7_000539"
     CDS             34675..35634
                     /gene="ftsN"
                     /locus_tag="ACHUQ7_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFR1134096.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            35727..36257
                     /gene="hslV"
                     /locus_tag="ACHUQ7_000540"
     CDS             35727..36257
                     /gene="hslV"
                     /locus_tag="ACHUQ7_000540"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFR1134097.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            36267..37598
                     /gene="hslU"
                     /locus_tag="ACHUQ7_000541"
     CDS             36267..37598
                     /gene="hslU"
                     /locus_tag="ACHUQ7_000541"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFR1134098.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            37665..38591
                     /gene="menA"
                     /locus_tag="ACHUQ7_000542"
     CDS             37665..38591
                     /gene="menA"
                     /locus_tag="ACHUQ7_000542"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFR1134099.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            38684..39169
                     /gene="rraA"
                     /locus_tag="ACHUQ7_000543"
     CDS             38684..39169
                     /gene="rraA"
                     /locus_tag="ACHUQ7_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFR1134100.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(39254..39499)
                     /gene="zapB"
                     /locus_tag="ACHUQ7_000544"
     CDS             complement(39254..39499)
                     /gene="zapB"
                     /locus_tag="ACHUQ7_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFR1134101.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            39925..40770
                     /gene="glpF"
                     /locus_tag="ACHUQ7_000545"
                     /pseudo
     CDS             39925..40770
                     /gene="glpF"
                     /locus_tag="ACHUQ7_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
     gene            40810..42300
                     /gene="glpK"
                     /locus_tag="ACHUQ7_000546"
     CDS             40810..42300
                     /gene="glpK"
                     /locus_tag="ACHUQ7_000546"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFR1134102.1"
                     /translation="MVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDP
                     MEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAIVWQCRR
                     TAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSHERARRGELLFGTV
                     DTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEV
                     YGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVK
                     SENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFATKVQNT
                     NGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQA
                     DSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGLAVGFWQ
                     NLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            42530..43540
                     /gene="glpX"
                     /locus_tag="ACHUQ7_000547"
     CDS             42530..43540
                     /gene="glpX"
                     /locus_tag="ACHUQ7_000547"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFR1134103.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            43637..44383
                     /gene="fpr"
                     /locus_tag="ACHUQ7_000548"
     CDS             43637..44383
                     /gene="fpr"
                     /locus_tag="ACHUQ7_000548"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFR1134104.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(44388..44816)
                     /gene="uspD"
                     /locus_tag="ACHUQ7_000549"
     CDS             complement(44388..44816)
                     /gene="uspD"
                     /locus_tag="ACHUQ7_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFR1134105.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(44843..45142)
                     /locus_tag="ACHUQ7_000550"
     CDS             complement(44843..45142)
                     /locus_tag="ACHUQ7_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFR1134106.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(45354..45794)
                     /locus_tag="ACHUQ7_000551"
                     /pseudo
     CDS             complement(45354..45794)
                     /locus_tag="ACHUQ7_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            45895..46494
                     /locus_tag="ACHUQ7_000552"
     CDS             45895..46494
                     /locus_tag="ACHUQ7_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFR1134107.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            46602..47369
                     /gene="tpiA"
                     /locus_tag="ACHUQ7_000553"
     CDS             46602..47369
                     /gene="tpiA"
                     /locus_tag="ACHUQ7_000553"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFR1134108.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(47424..48179)
                     /gene="cdh"
                     /locus_tag="ACHUQ7_000554"
     CDS             complement(47424..48179)
                     /gene="cdh"
                     /locus_tag="ACHUQ7_000554"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFR1134109.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGHTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(48286..49275)
                     /gene="sbp"
                     /locus_tag="ACHUQ7_000555"
     CDS             complement(48286..49275)
                     /gene="sbp"
                     /locus_tag="ACHUQ7_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFR1134110.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(49595..50557)
                     /gene="pfkA"
                     /locus_tag="ACHUQ7_000556"
     CDS             complement(49595..50557)
                     /gene="pfkA"
                     /locus_tag="ACHUQ7_000556"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFR1134111.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(50738..51640)
                     /gene="fieF"
                     /locus_tag="ACHUQ7_000557"
     CDS             complement(50738..51640)
                     /gene="fieF"
                     /locus_tag="ACHUQ7_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFR1134112.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(51788..52288)
                     /gene="cpxP"
                     /locus_tag="ACHUQ7_000558"
     CDS             complement(51788..52288)
                     /gene="cpxP"
                     /locus_tag="ACHUQ7_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFR1134113.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            52438..53136
                     /gene="cpxR"
                     /locus_tag="ACHUQ7_000559"
     CDS             52438..53136
                     /gene="cpxR"
                     /locus_tag="ACHUQ7_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFR1134114.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            53133..54506
                     /gene="cpxA"
                     /locus_tag="ACHUQ7_000560"
     CDS             53133..54506
                     /gene="cpxA"
                     /locus_tag="ACHUQ7_000560"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFR1134115.1"
                     /translation="MIGSLTARIFAIFWLTLVLVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(54612..55286)
                     /gene="yiiM"
                     /locus_tag="ACHUQ7_000561"
     CDS             complement(54612..55286)
                     /gene="yiiM"
                     /locus_tag="ACHUQ7_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFR1134116.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
BASE COUNT        13493 a        15017 c        14164 g        12744 t
ORIGIN      
        1 gaatgctgac aaatttctgc ctgatgcggc gtaaacgcct tatccgccta cgaagtgtcg
       61 cactttgtag gcgtggtaaa ctccccattt tcaggggagc aatataaaca aattactcta
      121 accgagcctg cgcaaaagca atcgcctgcg ccacctgctg cggtgagacg ccgccttttg
      181 ccgcacgctt ttcgaggcac gattgtagcg acagaatcgg atagacatct tcgccaatca
      241 cctgactgaa tttctgcaac tcactgagtg gcagatcttc cagcggtttg ccctgacgaa
      301 tggcttccac caccgcttca cccacaatat ggtgcgcctc gcggaaaggt acgcctttcg
      361 ccaccaggta atccgccagt tcagtggcgt tcgcataccc ctgctgcgcc gcttcctgac
      421 aacgcggacg tttcacctga atgccgtcca gtaccagcgc cgccatatgc aggcagtcca
      481 gccaggtatc gagcgcgtcg aacagacctt ctttgtcttc ctgcatatct ttgttgtaag
      541 ccagcggcaa acctttcagc gtcatcatca tgccggttaa cgcaccctgc acccggccgc
      601 atttaccgcg aatcagctcc agcgcatccg ggtttttctt ctgcggcatt aatgatgaac
      661 cggaagtcac gcggtccgaa agctccacaa accccgcttc gccggtgtta aagaaaatca
      721 gatcttcagc aaaacgcgac agatgcacca tgccgatagc ggcagcagaa agcagttcca
      781 acacatggtc acggtcagaa acgctgtcca gactgttacg ggtcgccgaa gcaaagccca
      841 gccagcctgc taactgttca cggtcgattt cataggccgt tcccgccagc gcgccacagc
      901 ctaatgggct gacatccaga cgcttaagcg catcctgcaa acggctttca tcacgcgcca
      961 gcatctcaac ataggccagg caccagtgcg cgaacgtcac cggctgggcg cgttgcaggt
     1021 gagtgtaacc tggcattacc gcgtcctgat tgttttgtgc ggtttccacc agcgccgatt
     1081 gcagctgccg gttagccgtc agtaactcgc taacggtatc tttgcaccac agtttcaggt
     1141 cagtcgctac ctgatcatta cggctacgcc cggtatgcag ctttttgcct aactggccca
     1201 ctttgtcgat cagtttgcct tccacccagc tatggatatc ttcggcgtcg ctttcaagga
     1261 tttgttgtgg cctggcgcga acatcttcca gcaacacgtt cagcgcctct tccagttgcg
     1321 cctgctcttc tgcggttaac acgccgaccg ttaccaaggc tttggaccag gccacagagc
     1381 caacaatatc ctgctccgcc agacggtaat caaagcgcag tgagtcgttg aattgtttga
     1441 accgttgatc tgctgcctgg gtaaaacgcc cgccccaaag tgccataact ctgtttcctt
     1501 attttgaaat tcaatgccgg aaagctgcgc ctccggctaa caaaacttaa gctaaaatcc
     1561 gcgtacccat cggcataccg ttaaacagtg ccggaagctg ctccgcataa cgccaagaag
     1621 cgatatctac cggacggccc agcgtgcggg ccgcatccag cgccgcgttc actttcacta
     1681 tcatgccgtc agtaataatg ccctgctcaa tcagttgttc tgctttcgcg gcggtcattt
     1741 cggcaatgcg ttgccctttg ccgtcgagaa tgccgctgac gtcggagagc aaaatcagat
     1801 ccgcgcccag cgttgccgcc agcgccgttg ccgcctggtc ggcattgacg ttcatcagtt
     1861 gcccttcgtc tgttacgcca atggagctga ccaccggcag ataaccgttc tccagcaagg
     1921 agttgataag cttaggcgaa cctggctgcg ccagtccaac atgacctaac tcttcatcaa
     1981 gctgggtcac tttgacgctg tcgccgtcac cgagaaacaa acctacggcc gcaatctgat
     2041 gtttcttcgc ccatgccaac agggttttat ttgccgttcc cgccagtgct ccggtgataa
     2101 tgtctatctg atcagcaggc gtcacccgca ggccgttttt ctttttcacc ggcagattca
     2161 gccctttcat cagctcatcc accacgcaac cgccgccgtg cacaatcacc agcggacgct
     2221 gatgtgactc acgataattc accaatgcgc taaacagacg ttccagcgcc tcttcactat
     2281 ccagcagtac gccgcccagt ttgataatta atggattcat cattgcaccc ttaaataaga
     2341 gactgcgttt ccgcatagcc gaaacgaata ttggcgcact gtaccgcttg tgccgccgcg
     2401 cctttcagta agttgtcttc ggtcgccaca ataatcagat gctcgccctg aacggcaaac
     2461 ccgatatcgc aaaatggcag cccaacgaca tttttcagcg ccggaacgcc tttgtcatac
     2521 agccgcacca gcggtttatg ggcatacgcc tgctgtaacg cttgcgcgac ttgcgcctgg
     2581 gtcacacccg atttcaggcg gcaggtaatg gtttcgagaa tgccgcgcgg gaaattgccc
     2641 agatgtgggg tgaagataac gtcagcaccg aggtgtgtgg cgatctctgg ttgatggcga
     2701 tgagtaaaga cgccatacgg ttgcaggcta acttcacaaa aactatttga aatcgctgcc
     2761 ttacgtcctg caccgctcac gccgctggtg gcgttgatca ccggccactg attgaggtca
     2821 agaagatcgg catcaatcaa cggtttcagc gccagctgtg ccgccgtcgg ataacagcct
     2881 ggcaccgcaa tcaaattcgc ttcttttaat ttattaccgc accactccgc cagaccgtag
     2941 gctgcctgtt ccagcagttc cgggtattga tgggtaaagc cgtaatattt ttcatagaag
     3001 gtggcgtcgt taacacgaaa cgcgccggaa aggtcgaaca ccacgcagcc cgcttcaaga
     3061 aactgcggcg ctaaatcgtg gctaacttca tgggcggtgg cgagaaacac tacgtccacc
     3121 cctgggctaa actcgctgat atccgacatc ggctgcaacg gcagctcaac gatgcctttt
     3181 agctgcggat gcaaatcgga gattaacttt cccgcatcat tgctttgcgc tgaaacagtc
     3241 aaagcggtta tgttcatatg cggatggcga tttacatagg tcactagctc tgcgccagcg
     3301 tagccgctgg cacccacaat cagcgtattc aacatcgggg ctattcacct tcttatgtct
     3361 ggttgccagg ttaaacgtaa aacattcacc ttacggctgg tgggttttat tacgctcaac
     3421 gttagtgtat ttttattcat aaatactgca tgaatattga tactatcatg accagaggtg
     3481 tgtcaacaat gaaaaacaaa ttaccgccat ttatcgagat ttaccgcgct ctgattgcca
     3541 caccttcaat aagcgctacg gaagaggcac tcgatcaaag caatgcagat ttaatcactc
     3601 tgctggcgga ctggtttaaa gatttgggct tcaatgtgga agtgcagcct gttccaggaa
     3661 ctcgcaacaa atttaatatg ctggccagtt acggacaggg ggctggcggc ttgttgctgg
     3721 cggggcatac cgatacggtg ccatttgatg acggtcgctg gacgcgcgat ccgtttacac
     3781 tgacggagca tgacggcaag ctttacggct taggcaccgc cgacatgaaa ggcttttttg
     3841 cgtttatcct tgatgcgcta cgcgatgtcg acgtcacgaa actgaaaaaa ccgctctaca
     3901 ttctggcgac tgctgatgaa gaaaccagta tggccggagc gcgttatttt gccgaaacta
     3961 ccgccctgcg cccggattgc gccatcattg gcgaaccgac gtcactacaa ccggtacgcg
     4021 cacataaagg tcatatctct aacgccatcc gtattcaggg ccagtcgggg cactccagcg
     4081 atccagcacg cggaattaac gctatcgaac taatgcacga cgccatcggg catattttgc
     4141 aattgcgcga taacctgaaa gaacgttatc actacgaagc atttaccgtg ccatacccta
     4201 cgctcaacct cgggcatatt cacggtggcg acgcttctaa ccgtatttgc gcttgctgtg
     4261 agttgcatat ggatattcgt ccgcagcctg gcatgacact caatgaactt aatggtttgc
     4321 tcaacgatgc attggctccg gtgagcgaac gctggccggg tcgtctgacg gtcgacgagc
     4381 tgcatccgcc gatccctggc tatgaatgcc caccgaatca tcaactggtt gaagtggttg
     4441 agaaattgct cggagcaaaa accgaagtgg tgaattactg taccgaagcg ccgtttattc
     4501 aaacgttatg cccgacgctg gtgctcggtc cgggttccat caatcaagct catcagcctg
     4561 atgaatatct ggagacgcgg tttattaaac ccacccgtga gttgattatc caggtaattc
     4621 accatttttg ctggcattaa aacgtaggcc ggataaggcg ctcgcgccgc atccggcacc
     4681 gttgccaaac tccggtgccg caataatgtc ggatgcgatg cttgcgcatc ttatccgacc
     4741 tacacctttg gtgttatgta tggcgatttt ttaacatttc cataagttac gcttatttaa
     4801 agcgtcgtga atttaatggc gtaaattcct gctatttatt cgtttgctga agcgatttcg
     4861 cagcatttga cgtcaccgct tttacgtggc tttataaaag acgacgaaaa gcaaagcccg
     4921 agcatattcg cgccaatgcg acgtgaagga tacagggcta tcaaacgata agatggggtg
     4981 tctggggtaa tatgaacgaa caatattccg cattgcgtag taatgtcagt atgctcggca
     5041 aagtgctggg agaaaccatc aaggatgcgt tgggagaaca cattcttgaa cgcgtagaaa
     5101 ctatccgtaa gttgtcgaaa tcttcacgcg ctggcaatga tgctaaccgc caggagttgc
     5161 tcaccacctt acaaaatttg tcgaacgacg agctgctgcc cgttgcgcgt gcgtttagtc
     5221 agttcctgaa cctggccaac accgccgagc aataccacag catttcgccg aaaggcgaag
     5281 ctgccagcaa cccggaagtg atcgcccgca ccctgcgtaa actgaaaaac cagccggaac
     5341 tgagcgaaga caccatcaaa aaagcagtgg aatcgctgtc gctggaactg gtcctcacgg
     5401 ctcacccaac cgaaattacc cgtcgtacac tgatccacaa aatggtggaa gtgaacgcct
     5461 gtttaaaaca gctcgataac aaagatatcg ctgactacga acgcaaccag ctgatgcgtc
     5521 gcctgcgcca gttgatcgcc cagtcatggc ataccgatga aatccgtaag ctgcgtccaa
     5581 gcccggtaga tgaagccaaa tggggctttg ccgtagtaga aaacagcctg tggcaaggcg
     5641 taccgaatta cctgcgcgaa ctgaacgaac aactggaaga gaatctcggc tacaaactgc
     5701 ccgtcgaatt tgttccggta cgttttacct cgtggatggg cggtgaccgc gacggcaacc
     5761 cgaacgtcac tgccgatatc acccgccacg tcctgctgct cagccgctgg aaagccaccg
     5821 atctgttcct gaaagatatt caggttctgg tttctgaact gtcgatggtt gaagcgaccc
     5881 ctgaactgct ggcgctggtt ggcgaagaag gtgccgcaga accgtatcgc tatctgatga
     5941 aaaacctgcg ttctcgcctg atggcgacac aggcatggct ggaagcgcgc ctgaaaggcg
     6001 aagaactgcc aaaaccagaa gggctgctga cacaaaacga agaactgtgg gaaccgctct
     6061 acgcttgcta ccagtcactt caggcgtgtg gcatgggtat tatcgccaac ggcgatctgc
     6121 tcgacaccct gcgccgcgtg aaatgtttcg gcgtaccgct ggtccgtatt gatatccgtc
     6181 aggagagcac ccgccatacc gaagcgctgg gcgagctgac ccgctacctc ggtatcggcg
     6241 actacgaaag ctggtcagag gccgacaaac aggcgttcct gatccgcgaa ctgaactcca
     6301 aacgtccgct tctgccgcgc aactggcaac caagcgccga aacgcgcgaa gtgctcgata
     6361 cctgccaggt gattgccgaa gcgccgcaag gctccattgc cgcctacgtg atctcgatgg
     6421 cgaaaacgcc gtccgacgta ctggctgtcc acctgctgct gaaagaagcg ggtatcgggt
     6481 ttgcgatgcc ggttgctccg ctgtttgaaa ccctcgatga cctgaacaac gccaacgatg
     6541 tcatgaccca gctgctcaat atcgactggt atcgcggcct gattcagggc aaacagatgg
     6601 tgatgattgg ctattccgac tcagcaaaag atgcgggcgt gatggcagct tcctgggcgc
     6661 aatatcaggc acaggatgca ttaatcaaaa cctgcgaaaa agcgggtatt gagctgacgt
     6721 tgttccacgg tcgcggcggt tccattggtc gcggcggcgc acctgcccat gcggcgctgc
     6781 tgtcacaacc gccaggaagc ctgaaaggtg gcctgcgcgt gactgaacag ggcgagatga
     6841 tccgctttaa atatggtctg ccagaaatca ccgtcagcag cctgtcgctt tacaccgggg
     6901 cgattctgga agccaacctg ctgccaccac ctgagccgaa agagagctgg cgtcgcatta
     6961 tggatgaact gtcagtcatc tcctgcgatc tctaccgtgg ctacgtacgt gaaaacaaag
     7021 attttgtgcc ttacttccgc tccgctacgc cggaacaaga attgggcaaa ctgccgttgg
     7081 gttcacgtcc ggcgaaacgt cgcccaaccg gcggcgtcga gtcgctgcgc gccataccgt
     7141 ggatttttgc ctggacgcaa aaccgcctga tgctccccgc ctggctgggt gcaggtacgg
     7201 cgctgcaaaa agtggtcgaa gatggcaaac agagcgagct ggaagccatg tgccgcgatt
     7261 ggccattctt ctcgacgcgt ctcggcatgc tggagatggt cttcgccaaa gcagacctgt
     7321 ggttggcgga atactatgat caacgtctgg tagacaaagc actgtggccg ttaggtaaag
     7381 agttacgcaa cctgcaagaa gaagacatca aagtggtgct ggcgattgcc aacgattccc
     7441 atctgatggc cgatctgccg tggattgcag agtctattca gctacggaat atttacaccg
     7501 acccgctgaa cgtattgcag gccgagttgc tgcaccgctc ccgccaggca gaaaaagaag
     7561 gccaggaacc ggatcctcgc gtcgaacaag cgttaatggt cactattgcc gggattgcgg
     7621 caggtatgcg taataccggc taatcttcct cttctgcaaa ccctcgtgct tttgcgcgag
     7681 ggttttctga aatacttctg ttctaacacc ctcgttttca atatatttct gtctgcattt
     7741 tattcaaatt ctgaatatac cttcagatat ccttaaggaa ttgtcgttat attcggcgat
     7801 atttttttca agacaggttc ttactatgca ttccacagaa gtccaggcta aacccctttc
     7861 tagctggaaa gccctgggtt gggcactgct ctacttttgg tttttctcta ctctgctaca
     7921 ggccattatt tacatcagtg gttatagtgg caccaacggc attcgcgact cgctgttatt
     7981 cagctcgctg tggttgatcc cggtattcct ctttccgaag cggattaaaa ttattgccgc
     8041 agtgatcggc gtggtgctat ggacggcctc tctggcggcg ctgtgctact acgtcatcta
     8101 cgggcaggag ttctcgcaga gcgttctgtt tgtgatgttc gaaaccaaca ccaacgaagc
     8161 cagcgagtat ttaagccagt atttcagcct gaaaattgtg cttatcgcgc tggcctatac
     8221 ggcggtggca gttctgctgt ggacacgcct gcgcccggtc tatattccaa agccgtggcg
     8281 ttatgttgtc tcttttgccc tgctttatgg cttgattctg catccgatcg ccatgaatac
     8341 gtttatcaaa aacaagccgt ttgagaaaac gttggataac ctagcctcgc gtatggagcc
     8401 tgccgcaccg tggcaattcc tgaccggcta ttatcagtat cgtcagcaac taaactcgct
     8461 aacaaagtta ctgaatgaaa ataatgcctt gccgccactg gctaatttca aagatgaatc
     8521 gggtaacgaa ccgcgcactt tagtgctggt gattggcgag tcgacccagc gcggacgcat
     8581 gagtctgtac ggttatccgc gtgaaaccac gccggagctg gatgcgctgc ataaaaccga
     8641 tccgaatctg accgtgttta ataacgtagt tacgtctcgt ccgtacacca ttgaaatcct
     8701 gcaacaggcg ctgacctttg ccaatgaaaa gaacccggat ctgtatctga cgcagccgtc
     8761 gctgatgaac atgatgaaac aggcgggtta taaaaccttc tggatcacca accagcagac
     8821 gatgaccgcc cgcaatacca tgctgacggt attttcgcgc cagaccgaca agcagtacta
     8881 catgaaccag caacgtacgc agagtgcgcg tgaatacgac accaacgtgc tgaagccgtt
     8941 ccaggatgtg ctgaatgacc ctgcgccgaa gaaactgatc atcgttcatc tgctgggtac
     9001 gcatatcaaa tacaaatacc gctacccgga aaatcagggc aagtttgatg gcaataccga
     9061 tcatgttccg ccaggattaa gcgcagaaga gctggaatca tataacgatt atgacaacgc
     9121 taacctgtat aacgatcatg tggttgccag cctgattaaa gactttaaag cggcagaccc
     9181 gaacggattc ctggtttact tctctgacca cggtgaagag gtttacgaca cgccgccgca
     9241 caaaactcag gggcgtaacg aagacaaccc gacgcgccac atgtacacca ttccgttcct
     9301 gctgtggacg tcggaaaaat ggcaagcgac tcatccccgt gatttctcac aggatgtcga
     9361 tcgtaaatac agcctggcgg aactgatcca cacctggtca gatttggcgg gcttatctta
     9421 cgacggttac gatccaaccc gttcagtggt gaatccgcag ttcaaagaaa ctacccgctg
     9481 gattggtaac ccgtacaaga aaaacgcgct gatcgattac gacactctgc cgtatggcga
     9541 tcaggtgggt aatcagtaga tgacctttgt atcgcgtctg gcgctatttg ccggatgcga
     9601 tactgccgca atgtttacat cgaactcccc tcaggccaca cttttcaggt ggcctttttt
     9661 aataatgcca gttactgttt tcccgtctac atacctaagg gaataacatg tatcacgacg
     9721 tcagctatct gctctcccgc ctgatcaacg gcccgctgtc cctgcgccag atttactttg
     9781 ccagcagtaa cggcccggtg cccgatcttg cgtatcaggt cgattttcca cggctggaaa
     9841 ttgtgctgga aggtgagttt gttgataccg gcgctggagc aacgttagtt cccggcgatg
     9901 tgctgtacgt tgccgctggt ggctggaatt tcccacaatg gaaaacaccc gctaccacct
     9961 ttagcgtgct gtttggcaaa cagcaactcg gcttcagcgt cgtgcaatgg gatggcaaac
    10021 aatatcaaaa tctggcgaag caacacgtcg cccgacgcgg cccacgcata ggttcttttc
    10081 tgctacaaac gctcaatgaa atgcaaatgc agccgcagga gcagcaaacg gcaaggctta
    10141 tcgtagccag cctgcttagc cactgccgcg atttgctcgg cagccaaatc cagaccgcct
    10201 cacgcagcca ggcactattt gaagctattc gcgattatat cgacgaacgc tatgcctccg
    10261 cgcttacccg cgaatctgtc gcacaggcat tctatatttc accaaactat ctctcgcacc
    10321 tgtttcaaaa aacgggggcc attggtttta acgaatacct gaatcacacg cgactggagc
    10381 acgctaagac gttattgaaa ggctatgacc tgaaggtaaa agaagtggcg cacgcctgcg
    10441 gctttgtcga cagcaactac ttctgccgtc tgtttcgtaa aaacaccgaa cactcgccgt
    10501 cggaatatcg ccggcagtac cacagccaac tgacagaaaa accgactact ccagaataag
    10561 atgggtttgc tgcggcgcag aaagcacttt gcgcaccgcg ctcattaccc gctgcggctc
    10621 acgcaggaat gcgtagatgc tgcattgcac atagcggcaa tgttcaaatc gctcggcacc
    10681 ggcaagctcg atatccgtaa tcaacagagc aacatccgcc cgacggatat cctcgtccgc
    10741 taaacgattc tccgttccca gcgcgccctg agtttcaatg ctgacgcccc acttctctaa
    10801 ctggcacaac ttttccagcc gttccgccgc catataagta tgcgccacgc cgcttacgca
    10861 ggcggttact gccaccaggt atgccatttt aacctcccac ggtaacctga aatccggccc
    10921 gttctgccat ttcgcgcatc gttgtcacat cggctgacga cggcgcaggc acctctttca
    10981 tcgaccatgt tttccccagc aggcggtatt tcggttcgcc gtactgatga aacggtaaca
    11041 gatggatctg cctgattttc agcgggatca gtacatccag cgcctgctgc atattctccc
    11101 ggctgagcgt gaaaccaggg atcaacggta aacgcgggat cacgttgaca ccctcactca
    11161 ccagcaaacg cagattctcc agcacgcgtg gcaggttcat cttcaccaca tcccgcgcct
    11221 gagtcgcgtc cataattttt aaatcgaaca gcacttcatc gcacaatttc gccagcggta
    11281 acagcttgct tgctggcgcg tctccggcag tttcaatggc gcatgagaca ccccacagcc
    11341 gcagtcgctg taaaaaacgg gtagcaaact ccgcctgcat taacacttcg ccgccagaaa
    11401 gcgtgacgcc gccgccggac gtgcgaaaga aaatgtcatc tttcatcact tcccgttcca
    11461 gagcgtcaag gctgatatcg cgaccaatcc gttcaaacgc cccggagggg cattcatccg
    11521 catcacgcaa acatttcgca cagtgcagac atttcgcctc tctgcgtacc gtctggattt
    11581 tgccggagat cgactccgga ttagcacacc acgggcaaag atgcggacag cctttaaaaa
    11641 agaccaccgt acgaatgccc tcgccgtcat tcagtgaata acgctgaatg ttgaaaatgc
    11701 gcgccacatc atcgcggcgc gtttccacaa cgttacagct gatgcgctgt ccggcggatg
    11761 atgtcatcct ggatctcctt cgacagttcg acaaagaagg cgctgtatcc ggcaacgcgc
    11821 accaccagcc cggcataatc ttgtgggcgc tgttgcgctt cccgcaacgt gtcggcgttc
    11881 accacgttaa actgaatatg ctgcaactta agctgggtaa acacccgtaa gaagtcggcc
    11941 agtttgcgta atcccgcttc accttccagg gtcgccggag tgaatttcac gttcagcaac
    12001 gtaccgttag acagcagcgt attatcgagc ttactgactg acttcagtac cgccgttggc
    12061 ccttgtgcgt cctgacccag cataggtgac aagccgccgt ctgccagttg ttccccggca
    12121 aaacgaccgt ctggcgtcgc gccaaccacc gatcccaacg gaacgtgagc agaaacggta
    12181 tacgatcccg gcgtgaagta accgccgcgc gggttctggt atttttccac ttctttgcag
    12241 tagtggcgca acagttcggc gctaatgtta tccacctcgt cgatatcgtt accgtatttc
    12301 tcaaagcggt taattaagcg agcgcggact ttttcgcctt ctggcgttgc aaagttggct
    12361 tttaataccg acagcaattc gtcaaaactt aaacgctgtt gctcaaaaac catccctttc
    12421 aacgcatgga gagaatcgct caggttggcg ataccaattc cctgtacgcc ggagaagtta
    12481 taacgcgcgc cgccatcggt aatatcgcga cctttttcca gacaatcgct gataaaagac
    12541 gagagcagcg gtacaggtgc ccagtcgcga tggccgatat cacaaatatt gctgccctca
    12601 accatcaggg tgatgtagtg gctgatcttg gcgcggatct gctccagtaa accttcataa
    12661 gtcagcgcag cattgccttc attttcatgc aggcagattt ccatcacttt cagcagatta
    12721 aacatcgcga tgtcatgcaa gccgtaggtt ctgccgggaa tagataattc cacacagccc
    12781 actacggaat agtcgcgcgc atcttccagc gaaacgccac ggttgaggaa cgctggcacc
    12841 accacttcat cgttaaagat ttgcggaata ccggtgccga ggcgaatggt ttcggcggtt
    12901 ttcatcagga acggcgtgtc gatcaaggcg ttagtgcgca cgccgaggtt cggttgcggt
    12961 aattgcacgc tttgataggc gtcaaggcaa aggaacgaaa gcacgttcac cgcgctacgt
    13021 ccgttctcgg ttaacccgcc gagcagtgcg gtatagccgg tcgggaaacc ggcgaaataa
    13081 cgcgcgctac tggtggagcg caacagcacg atgtcgttgc atttcaccca taaagattcg
    13141 agcagttctt tcaggaacgc cggatcttcg ccctgggtta aagatgcctg atagaacggc
    13201 aacatatact ggtcgaagcg ccctaaagac agcgaactgg cgttggattc gtattgcaga
    13261 atgatgttca tgtaccagaa taactggcac gcctgccaga acgtctgcgg cttatgttgc
    13321 gcgttatggc gggagatctc cgcaatagtc agcagctctt cgcgacgctg ggcatctgtg
    13381 cagtttgccg ccatcgtttc cgccagttcg gcgtaacgca aaatgtgttt ctgcgaggct
    13441 tccagcagta acaacgcggc ctgataaaag tgattctccg gctgttgctg acaatgttgc
    13501 tgcatctgtg ccaccagctc acccagaccg tgattcaaca ggcgtgggta atcaataata
    13561 atgtgcccct ggcctttatc cgtctggttg atgctgaaaa tctgcgtgtt ggtcgcggct
    13621 tttacttcat ctgtcatctg cccgttgatg aaatctttca tcgaacgttt ttcccagtac
    13681 gggaacaact cttcacggta gatacgttta tcttcttcgc tgatagcaaa gcggtcctgc
    13741 ggacgcgtcg ggaattgatc cagctctttc agcagccagt aagggtccat ttccggcgac
    13801 ataatcccgg cgcgcggttt tacggtgcgg ttaccggcaa tcagttcttc atcacgaatc
    13861 gaaatttcaa catgttcaag gatatacgct gttgctttcg cccggcgcaa tatcaccggt
    13921 tcgccttcgg tttgccgatg gctggcggta taaagcagcg cccgctccag cgagatttca
    13981 cgggtattgg caaacagtgc agttttgagg cgagagatac gattcgtcat gaggaactcc
    14041 tgtgggaaag cgaacccgcc cttgcgagcg ggagaagaac ttaagcggtt tgcgccaggt
    14101 gagcttcaat tttgctcata atggcgtcag cacgttttac tgcatcgctg atgttgacgc
    14161 gcacgatggt cttgccagca aaacgctctt caaacttgat gccgatatct ttggtcagaa
    14221 tcaccatatc agcgctcgcc acgtcttccg cagtcagttc attttccaga ccaattgacc
    14281 cctgggtttc tactttcact tcccagcctt tcgctttcgc ggcactttcc agcgcctctg
    14341 ctgccatata ggtatgggca acacctgaag gacatgcggt tactgcaata attttcgtca
    14401 tggctcgttc ctcaattaat taatttcaaa atccaaatcc aggtcgtctt ctttttcatc
    14461 agtcgaagac ccatttttac gcgccagact tttcagcacg ttcacacaaa cagccgtcac
    14521 cactgctccg acagccactg ctgcgatata acccaactta ccttcaacca ccggcagcac
    14581 aatcagacca ccccagcctg catagcactg cgcaccgacc agcgccgcag ttactgcacc
    14641 acaaactgaa ccgaccataa tggaaggaat gacacgcagc ggatcggcag cggcgaaagg
    14701 aatcgcccct tcagtaacgc caacacagcc catcaccagt gccgctttgc cggtttcgcg
    14761 ctcttctgcg gagaaatttt tacgaccaat cagcgtcgcc agccccatcc ccagcggcgg
    14821 gatacaaata cccacggcag caatggccac cacggtataa acaccctgag caacgcaaat
    14881 cagcatgaag gcataggcca ctttgttaac cggaccgccc atatcgaacg ccagcatcag
    14941 ccccatgatc accgccagca taacaatgct gccctgctgc atcccctgaa gccactgagt
    15001 caggctgttg gtcaacgccc ctacaggctc gcccaagccc cacatcatga tgcctgcggt
    15061 aatcagtgtg ccgatgatag gaataatgaa gatgggcatc accgagcgca gaactttatg
    15121 caccggaatt ttcttcaggt aatgcaccac gatgccgccg ataatcccgg cgatcagcgc
    15181 accaaagaag cccgcaccaa agctgttacc aacccaggca ccgatagcgc acggagccag
    15241 cgcagaacgc tctgcaatgg agtaaccaat gtaagcggcg aggaaaggca ccatcagcgt
    15301 caaacctgca acgccgatat caaacagttt tttcaaattc ggatcggcta cggcatccgg
    15361 cactgcgcct ttgccataca acatgacgga aaccgccagc aaaataccgc ccgataccac
    15421 gaagggaatc atgtgtgaaa cgcccgtcat taaatgctga cgggtatttt tcaggatctg
    15481 caccaactca ttcataagct gctcccgggc ttgcaaaagc ccatgtccat atttttgatg
    15541 gaggaaacaa taggcaatcg caggcggagc aaacagtgga taaaaaggcc atttactgga
    15601 cttttgtaat atcagtacaa aaatgcgatc cgcctcataa cttgcgataa tccggaagca
    15661 tgccgcaaaa cggcgtcttg tgagacgcgt cgcaaattga cggttacatt acatttgtcc
    15721 agcttttggc agatttgtca catggcaggc cgttaccgtg cgctcttatc ctgtaaacag
    15781 aaaaccgtac cgggagaaag gcaatggccc tgattgtgga atttatttgt gagctaccta
    15841 acggcgtaca tgcgcgtccg gcaagccacg ttgaaacgct gtgtaatact ttttcatctc
    15901 aaattgagtg gcataacctg cgtactgacc gtaaaggcaa cgccaaaagc gctctggcgc
    15961 tgattggcac cgatacgctg gtgggtgata actgccagct actgatttcc ggggccgacg
    16021 aacaggaagg gcaccagcgt ttaagccaat ggctgcgcga tgaattcccc cactgcgatg
    16081 cgccgctggc ggaagttaaa tctgacgaac tggaaccact gccggtttca ctgaccaatc
    16141 tgaatccgca aattatccgc gcccgcaccg tgtgcagcgg tagtgcaggc ggcattctga
    16201 cgccgatctc ttctttagat ctcaatgcgc tgagtaatct tcccgcagcc aaagacgttg
    16261 acgccgagca atccgcactg gaaaacggcc tgacgctggt actgaaaaac attgagtttc
    16321 gtctgctgga tagcgacggt gctaccagcg cgattctgga agcgcaccga tccctggctg
    16381 gcgatacttc cctgcacgaa catttactgg caggcgtcag cgccggatta agctgcgccg
    16441 aagcgattgt cgccagtgcg aatcactttt gcgaagagtt tgcccgttcc agcagcagat
    16501 acctgcaaga acgtgccctg gacgtacgcg acgtctgctt ccagttactc cagcaaatct
    16561 acggtgagca acgcttcccg gcaccgggca aactgacgca gtccgccatt tgtatggctg
    16621 atgaactgac ccccagccag ttcctcgaac tggataaaaa tcacctcaaa ggattgttac
    16681 tgaaaagcgg cggcaccacc tcacatacgg tgatccttgc ccgttcgttc aacattccga
    16741 cgctggttgg tgtggatatt gatgccctca ctccgtggca gcatcaaacg atttatatcg
    16801 acggcaacgc cggggcgatt gtggttgagc caggagaagc cgtagctcgt tattatcagc
    16861 aagaagcccg cgtacaggac gccctgcgtg agcaacagcg tgtctagctg acccaacaag
    16921 cccgtaccgc tgacggtatc cgcattgaaa ttgccgctaa catcgctcac tccgtggaag
    16981 cgcaggccgc attcggcaat ggtgcggaag gcgttggttt gttccgcact gaaatgctct
    17041 atatggatcg caccagtgca ccgggcgaaa gcgagctgta caacattttt tgtcaggcgc
    17101 tggagtctgc caacggacgc agcattattg tgcgcactat ggacattggc ggcgacaaac
    17161 ccgttgatta tctaaacatt cccgcagagg caaacccgtt cctcggttat cgcgccgtgc
    17221 gtatttatga agaatacgcg tcgttattta ccacacaact acgctcgatc ctgcgcgcct
    17281 ccgctcacgg cagcctgaaa atcatgatcc cgatgatctc ctcaatggaa gagatcttat
    17341 gggtgaaaga aaaactggca gaagccaaac agcaactacg taacgaacac attccgtttg
    17401 atgagaagat ccagctcggc attatgctgg aagtgccgtc ggtgatgttc atcatcgatc
    17461 aatgctgcga agagattgat ttctttagta ttggtagtaa tgacctgacg caatatctgc
    17521 tggcagtgga tcgcgataac gctaaggtta ctcgtcacta caacagcctg aatccggcat
    17581 tcttgcgggc gctcgattac gacgtgcagg cggtgcatcg ccagggtaaa tggattggtc
    17641 tgtgcggtga gctgggagcg aaaggttccg tgctgccgtt gctggtcggc ttagggctgg
    17701 atgaactcag catgagcgca ccatcaattc cggcggcgaa agcgcggatg gcgcaacttg
    17761 atagccgtga gtgccgcaag ttgctcaacc aggcaatggc ctgccgtact tcgctggaag
    17821 tggaacacct gctggcgcaa ttccgcatga cccaacagga cgcaccgctg gtcaccgccg
    17881 agtgcatcac gctggaaagt gactggcgca gcaaagaaga agtgctcaaa ggcatgaccg
    17941 ataacctgct gctggcgggc cgctgccgct atccgcgtaa actggaagcc gacttatggg
    18001 cgcgcgaggc cgttttctct accggtctgg gctttagttt tgccattcca cacagcaaat
    18061 cagaacacat tgagcaatcc accatcagcg tggcgcgtct gcaagcgccg gtgcgctggg
    18121 gcgatgatga agcgcaattc atcattatgt taaccctgaa caaacacgct gcgggcgatc
    18181 agcacatgcg cattttctcg cgcctcgctc gccgcatcat gcacgaagaa ttccgtaacg
    18241 cgctggttaa cgccgcctct gccgacgcta tcgccagcct gctgcaacat gaactggaac
    18301 tgtaaaagga aacatcatgg aactgtatct ggacaccgct aacgtcgcag aagtcgaacg
    18361 tctggcacgc atattcccga ttgccggggt gacaactaac ccgagcatta tcgctgccag
    18421 caaggagtcc atctgggaag tgctgccgcg tctgcaaaaa gcgattggtg atgagggcat
    18481 tctgtttgct cagaccatga gccgcgacgc gcaggggatg gtggaagaag cgaagcgcct
    18541 gcgcgacgct attccgggta ttgtggtgaa aatcccggtg acttccgaag gtctggcagc
    18601 aattaaaata ctgaaaaaat agggtattac tacacttggc accgctgtat atagcgccgc
    18661 agaagggtta ttagccgcac tggcaggggc aaaatacgtt gctccgtatg ttaaccgcgt
    18721 agatgcccag ggcggggacg gcattcgtac ggttcaggag ctgcaaacgc tgttagaaat
    18781 gcacgcgcca gaaagcatgg tgctggcagc cagctttaaa acgccgcgtc aggcgctgga
    18841 ctgtttactg gcaggatgtg aatccatcac cctgccctta gatgtagcgc aacaaatgct
    18901 caacacccct gcggtagagt cagctataga gaagttcgaa cacgactgga atgccgcatt
    18961 tggcactact catctctaaa ggagcaatta tggaccgcat tattcaatca ccgggtaaat
    19021 acatccaggg cgctgatgtg attaatcgtc tgggcgaata cctgaagccg ctggcagaac
    19081 gctggttagt ggtgggtgac aaatttgttt tagtttttgc tcaatccact gtcgagaaaa
    19141 gctttaaaga tgctggactg gtagtagaaa ttacgccgtt tggcggtgaa tgttcgcaaa
    19201 atgagatcga ccgtctgcgt ggcatcgcgg agactgcgca gtgtggcgca attctcggta
    19261 tcggtggcgg aaaaaccctc gatactgcca aagcactggc acatttcatg ggtgttccgg
    19321 tagcgatcgc accgactatc gcctcgaccg atgcaccgtg cagcgcattg tctgttatct
    19381 acaccgatga gggtgagttt gaccgctatc tgctgttgcc aaataacccg aatatggtca
    19441 ttgtcgacac caaaatcgtc gcgggcgcac ctgcacgtct gttagcagcg ggtatcggcg
    19501 atgcgctggc aacctggttt gaagcgcgtg cttgctctcg tagcggcgcg accaccatgg
    19561 cgggcggcaa gtgcacccag gcggcgctgg cactggctga actgtgctac aacaccctgc
    19621 tggaagaagg cgaaaaagcg atgcttgccg ccgaacagca tgtagtaacc ccggcgctgg
    19681 agcgcgtgat tgaagcgaac acctatttga gcggcgttgg ttttgaaagc ggcggctggc
    19741 ggcggctcac gcggtgcata acggcctgac cgctatcccg gacgcgcatc actattatca
    19801 cggtgaaaag tggcatttgg tacgctgacg cagctggtac tggaaaacgc gccggtggag
    19861 gaaatcgaaa ccgtagctgc acttagccat gcggtaggtt tgccaataac tctcgctcaa
    19921 ctggatatta aagaagatgt cccggcgaaa atgcgcattg ttgcaaaagc atcttgtgca
    19981 gaaggtgaaa ccatccacaa catgcctggc ggcgcgacgc cagatcaggt ttacgccgca
    20041 ctgctggtag ctgaccagta cggtcagcgt ttcctgcaag agtgggaata acctactcca
    20101 aactcccggc ttgtccggga gtttgaacgc aaaattgcct gatgcgctac gcttatcagg
    20161 cctacgcaat ctctgcaata tattgaattt gcgtgctttt gtaggccgga taaggcgttc
    20221 actccgcatc cggcactctg tgccagtaat ctgaaactcc cacgttgttc aggagttttt
    20281 tatccgcttc tggacatttt ctccacagaa caatcgttag cccggaaggt cgaatctgtg
    20341 actcttattt tcacggacga accagatgaa agcttcactg gcgctgctca gcctgctaac
    20401 ggcatttacc agccactcct taaaatctcc cgccgttcca ccaaccgtgg tacaaatcca
    20461 ggccaatacc aaccttgcca tcgcagacgg tgccagacag caaattggca gcacgctatt
    20521 ttacgatcgc agcttaccta tcctggcggt gatgttccgc aagaacgcgg tgtatgttcc
    20581 gatatggtga tccgcgcatt gcgcagccag aaggtcgatt tgcagaaact ggttcacgaa
    20641 gatatggcga agaattttgc tgagtacccg caaaaatgga agttaaagcg cccggacagc
    20701 aacatcgatc accgtcgggt gcctaatctg gaaacctggt ttagccgcca caacaaaaca
    20761 cgccccatca gtaaaaatgc cggtgactat caagcgggcg atattgtctc ctggcgatta
    20821 gataacgggc tggcgcatat tggggtggcg tcagatggct tcgcccgcga cggtacgccg
    20881 ctggtgatac ataacattgg cgcaggcgcg caggaagaag atgtgctgtt tagctggcgg
    20941 atggtggggc attatcgtta ttttgttaaa taagagatcg ccccacatga agaaaaaatt
    21001 cagatgcttt ttacactgac agcttctttt ttcttacggc tcaccagcgc cagtgccagc
    21061 acaatcagca caataccgct accttcaacg gcccccggat tctcaccgag cagccaccag
    21121 gaaaagagta cgccacaaac gggaacggcc agtgtgctta agctggcaat actggcaggc
    21181 aagtttttca gcacaaacag ccataagctc cacgctagcg ccgtcgccag aatcgcactg
    21241 taggccagcg cccagaacac ggtgggctgc cagtcaattt cacgttgcgg caccagtaaa
    21301 gcgaccacac tcatcaccag cgccgcatac agcatctgcc aggatgttaa cgataataaa
    21361 tccacgcgcg gatgacgggc gtataggcgt ttagcaacaa tcgcgctcgc cccccaacta
    21421 acaccggaga ggattgccag catggcactt ttcatcgaag agaaatcgag ttgccacggc
    21481 tgcaacacca aaaataagcc gaaagcggca atcagaatcg cgaaatattg cccacgtcgc
    21541 aggcgttcac cgagaaacaa cgcggcgaaa atcaccaccc agaacggcat ggtatagctc
    21601 aggatcgcca ccttccccgc acctccgctg accaacgccc actgcgccag accaaccatc
    21661 ccgcaggttt gtaacagggc aatggctaag gtgtatttaa acggcgtcgg gcgcattccg
    21721 cgaccacgta ataaaaggac gatgaataaa acgagagcgc cgaaaatgca gcgtaaggcg
    21781 gtaaagtcga aggcaccgat gtaactggtg acttgcttca tgaaaatcca gctataactc
    21841 cagataagtg tgagcacaac caagccactg attgccagtg ggttgctctt tcctgcggca
    21901 gacatgagag atccagtgtg tagttttcct gtggacacta tacgccggta aagaaacttc
    21961 agcgactgcc tgaaagacag tcgctgaaga aggtcagatt acagcaggtc gaaacggtcg
    22021 aggttcatca ctttcaccca tgccgctacg aagtctttaa caaacttctc gtgggcatcg
    22081 ctgctggcgt aaacttccgc tactgcacgc aggacagagt tagaaccaaa taccagatcc
    22141 gcacggctgg ctgtgtattt cacttcgccg gtttcgcggt cacgtccttc gaacagctct
    22201 ttcgattcgt cagtcgcttt ccactcgtaa cgcatatcca gcaagttcac gaagaagtca
    22261 ttgctcaata cgccaacgcg gtcagtgaag acgccgtttt tgctgccatc gaagttggca
    22321 cccagtacac gcatcccgcc caccagcgca gtcatttccg gcgcggtcag cgtcagttgc
    22381 tgtgctttgt cgatcagcag tgactcggtg gtggaaacgt ccagacgagc gcgatagtta
    22441 cggaaaccgt cagcaattgg ctccagcagc tcaaacatct caatgtcagt ctgatcctga
    22501 cgcgcatcaa cgcgacccgg cgcaaacggt acatgaatgc tcaaacctgc ggcgcttgcg
    22561 gctttctcaa caccaaccac accagccagc acgatgatat ccgccagcga ggctttacca
    22621 gactctttct ggattttctc cagaacaggc agagcacgaa cggctgcggc gttcacatcc
    22681 cagtcgcgct gcggcattaa tgccagacgc gcaccgttgg caccaccgcg tttgtcgcca
    22741 ccacggaagg tagaagcaga tgcccaggcc accgatacca gctcactaac agacagacca
    22801 gaatccgcaa tcgcgaattt cagatcgata atgtcctgct cggtcgggtt gtagatcggc
    22861 tgcggcagcg gatcttgcca gatcagatct tctttcggca cttccggccc gatgtagcga
    22921 gatttcggcc ccatatccct gtgcgtcagt ttgaaccagg cacgggcaaa ggcttcgttg
    22981 aacgcctgcg gatcgttgag gaaacgacga gagatcttct cgaactcagg atcaaaacgc
    23041 agcgtcaggt cggtcaccag cattgtcggt ttacgtttct tcgacggatc gaacggatcc
    23101 gggataattt ccggtgcgtc taccgcttcg aactggattg ctccagccgg actgcgggtc
    23161 tgtacccact catacttgaa cagattctcg aagaaatagt tgctccactg gatcggcgtc
    23221 tgggtccaga ctacttccag accagaggta atggcatctg cgccaacgcc gctgccgtaa
    23281 gtgctcgccc agcctaaacc ttgttcttca atcggtgcag cttctggatc aggacctacg
    23341 tttgatgtcg gaccggcacc gtgggtttta cccagcgtat gaccacccgc aatcagcgcc
    23401 acggtttctt cgtcgttcat gcccatgttg ccgaaggtcg cgcggatagc tgctgccgca
    23461 gaaagcggtt cgccgctgtg atccgggcct tccgggttaa cgtaaatcag gcccatctcg
    23521 gttgcaccca gcggtgcttt cgccagcgct tccggatgac ggtgagtcag ccaggctttt
    23581 tcatcacccc agttaacatc cagatccggt tcccagacgt cttcacgacc ggcaccaaaa
    23641 ccgaaggtac ggaagccgga gttttccagc gccacgttac ctgcgaggat aaacaggtcg
    23701 gcccaggaga ttttctgacc atatttctgt ttgattggcc acaacaggcg acgcgcttta
    23761 tcgaggctta cgttatccgg ccaggagttc agcggtgcaa aacgttgctg accacgaccc
    23821 gcgccgccgc gtccatcgat tgaacggtaa gtacctgcgc cgtgccaagc catacgaata
    23881 aacagaccgg cgtagctgcc ccagtcggct ggccaccacg gctgagattc tgtcagcagg
    23941 gctttcagat cttttttcag gccgtagtaa tctaatttgc tgaattcttt gcggtagtca
    24001 aagtcctcac ccagcgggtt agaacgatta gaatgttggt ttaacaggtc aacacggagt
    24061 tgatttggcc accagtcgcg agtggttgtg cccgcccccg cgctctggtc gtgaccgccc
    24121 tgatggaacg ggcatttgcc agtggctgtg gtgttatgga tatcgtctga cgtgctcatc
    24181 aatgtgctcc cctctacagt gttaccgtta cgatacacag cgttagagag aagttataat
    24241 tgaattaatc cacggatgcg atagttgaga tcttacatat tgttggttaa agagatgtag
    24301 atcaaattga tcgtaattaa gcttttttga ttatgtccga attcggacaa atgctttata
    24361 aaaaagggtt ttagcttcat atccttcaag taatgaggga gaaaacaagg ctgacaacct
    24421 tattttcccg ccctcatttc gaggcagcat tttgtgctct gtttaaaatt tgtgatcact
    24481 gtgtgatttt cacaaaagcc acactattta taaaccaggt cgaacaccca gcgtatggca
    24541 aatcgcgtaa ctcatttcag cgcggttaag cgtatagaag tggaaatctt tcactccttc
    24601 acggcttaaa atcttcacca tatccatggc aatattcgcg cccaccagtt tgcgggtttc
    24661 ggcatcgtca tccagcccgt caaacatttg cgccatccac gccggaatac gcacgttggt
    24721 catgtcggca aatttcttcg cctgtttaaa gttagatacc ggcaaaatgc ccgggatgat
    24781 ttccacatca atgcccgccg atacacagcg gtcacgaaaa cgcaggtagc tttcgacatc
    24841 gaagaagaac tgagtaatcg cgcggttggc tccggcatcc actttgcgtt tcagattaag
    24901 caaatccgcc tgagcgcttt ttgcttccgg gtgaacttcc ggatacgccg ccacggatat
    24961 atcgaaatct gccacttctt ttaacagcgt caccaggtca gaagcataca tttctggctt
    25021 accacttccc ggcggcagat cgccacgcag tgccacgata tgacgaatac cgttattcca
    25081 gtagtcgcgt gcaatggtgc gcagctcgtc gggcgtcgca tcaatgcaag taagatgcgg
    25141 tgccgcttcc agaccagtgc gatctttaat gcctttaata atgctgtgcg tacggtcgcg
    25201 ctcgccggag ttcgcgccat aggtcaccga tacaaacttc ggtttcaggc tgctaaggcg
    25261 atcgatggag ttccacaggg tctgctccat ttcactggta cgcggcggga aaaactcgaa
    25321 cgaaacgtta atctgcccct ggacttctgc caggctctga ttcagggcat cccgctggct
    25381 ggcgtgaaaa aagctcatac cttacctcat caatcgcttg tagttatatg ttgtgttgtg
    25441 cacatctatc catttagacg tccagatgta aagatgaagg aaaagccgaa gggcgtcaac
    25501 cgaaaaatga ccaatgatgg tgaggaaaaa tcagggaaga tgaaaaaact tcaggatggt
    25561 aagaaaaaga aaatccgata tgcaaagcaa accggactgc aaatataaac taaccatgta
    25621 cttattgctt cttgccggat tcgtcgtaaa cgcttcatcc ggcctaattc tatgataatt
    25681 caatatattt cattagaaac catagacccg aaaacatgag taccgggcat tattaaattt
    25741 ctgaaattac aacaactgtg ccagccggtt gatatccgac tgaatcgccc cggcggtgac
    25801 gtcgcgccca gcgccaggcc cacggatcac cagcggatta tcgcggtacc agcggctttc
    25861 gatggcaaag acgttatcgc atggcagcag tgatgccagc ggatagtctt cacgcaccgc
    25921 ctccacgccc acacgcgctt tgccgttggc atcgaaacgc gccacgtagc gcagcaccag
    25981 ccccatttcg cgggccgctt ccagccgttg caccatctgc tcgttaagtt catcgccatt
    26041 ttcaaagaaa tggtcgatgc tgccgccttc gcaatgagca ggcaccagcg attccacacg
    26101 tacctggtcc ggttcaatgt tgtaacccgc ttcacgcgcc agaatcacca gcttgcgcat
    26161 tacgtctttg ccggagaggt cgtcacgcgg atcaggctcg gttaagccct gctgccacgc
    26221 ctgatccacc agctcggtaa acggcacgct accgtcgaat tgcaggaaca gccaggagag
    26281 cgtgccggag aagatcccac tgatcgacaa aatggtatcg ccgctgtcga tcagatcgcg
    26341 cacggtatgg ttgatcggca agcccgcacc aacggtggcg ttgtatagcc agtgacgccc
    26401 ggttttttcg aaggcgtcgt ggatctggcg atatttattg ctgtcgcttg ctcccgccag
    26461 tttgttggca ctgatgacgt ggaaaccgtg gctggcgaaa tcgagatact gatcagcaag
    26521 ctgctggctg gcggtaacgt ccaacaccac taaatcatca tacggatggg cgcgcatcca
    26581 caggaaaagc gactcttcat cctgctcgac cgcttcatcg ttgaagaagg ctaacgcgcg
    26641 gctggcgtcc agcccgtcat aactcaacag actgcggcgg ctgtccacca cgcctgccag
    26701 cacaaattca aagccggtac gcgccgaaag cgtgctctgc tcgcgggcga acagttccag
    26761 ccagcgagag ccgatattgc ctttgccgaa caataccagg ccgatgcgtt tttctgcgcg
    26821 gaagacggac tgatgcagcc cctgaatcag gctttcggtc gggccggtgc gcagtactgc
    26881 caccagactg atgccgtcat cggactgcca cgtaaactcg accggctggc ctttcagttg
    26941 ctgccagaag cggtggcaat gcagcgggtt acgggtgaca cccgccccga ccatcgccac
    27001 cagcgccagc ccctgacgca ggcgcagttc tccaggtaat cccgcttcgt cgaggatttt
    27061 cagcgcactg tcggccactt ctgaggtgta gcaaaattgc aacaactggc gatcgttatg
    27121 cacgccaacc gccagtggac gcacctgggc acgtttcagg atctggtcga tctctttatg
    27181 cgccagtttg aaatcctgac tggcgggcac ctgaaactca atcaaacaga catcatcgtg
    27241 gctggtgaca atacgcgcac cagtaccgga ggccagcacg cgttcaatgc gcgtggaacc
    27301 ttgatccggc gtgtagctac agcgcagctg caggtcgatt tcgctgccag aaactggctg
    27361 taaagtacgg gcgtgaagaa cgggagccgc cagacgcgcc agttcgctgg cctcatccag
    27421 acgcagcaac ggcagcaggc aggcatcttt cactttacgc gggtcggcac tgtatacccc
    27481 ggcgacgtcg ctccagatgg ttacacgaga aacacccgcc agcgcaccga tttgtgtcgc
    27541 ggaatagtcg gaaccgttac gccccagcaa caccgtttca ccggcgttgt tacggctgat
    27601 aaacccggtc accaccagac gtttgctcgg atgttgtacc agtagctgtt gcagcaacgg
    27661 gtaagaaagc ccttcatcaa cctgcggttg cgcagcgcgt tcagcgcgta aaaactcacg
    27721 ggcatcaagc caggccgctg gcagcccttg ttgattaagt accgcagaca tcaggcgcgc
    27781 cgaccacact tcaccgtggc ccaccacttc cgcatacacc gcatcgttaa taccactgtc
    27841 gagcaacgcc gccaggcgct caagatcgct gacaaaagcg ctaatgaggc tatcggcttc
    27901 ttcagcgggt agcagaccgc taatcagatc gcactgatag cgacgcagcg tttgttgaac
    27961 ctgatgcgca gagagacgat cggtctggct tagtttcaac cagttaatca actggttagt
    28021 ggtgctaccg gcggcggaaa ccaccatcat atcgtcaggc tgagagaact ccgccataat
    28081 gcccgcgaca cgcaaataac acttcacatc agccagacta ctgccaccaa atttatgcag
    28141 ctgacgacct ttcgcccctg cctgcgcaat cacactcatt tttacccctt gtttgcagcc
    28201 cggaagccat tttccaggtc ggcaattaaa tcttcgccat cttcaatacc ggtggagata
    28261 cgcagcagcg tctcggagat cccggcggca gcacgcgctt ctggtgccat gcctgcatgt
    28321 gtcatggttg cggcgtgaga gattaaactt tccactcccc ctaatgattc cgccagcgta
    28381 aacaacgaca gcccgcccag gaaacgacac agcgtctgct catcgccatc cagttcaaaa
    28441 ctcaacattg cgccaaagcc tttttgctga cgcgcggcaa tttcatgccc ctgattttcc
    28501 ggcagcgacg ggtgatacag ttttttcacc aacggctggg tttgcagata tttcacaatc
    28561 gcctgcgcgt tgcgctgcgc cagctccata cgcggcacca gcgttcgcaa cccgcgtagc
    28621 agcagatagc tgtcaaacgc gccgcccgtc acgccaatat tgtttgccca ccaggcaagt
    28681 tcagtgacaa cgtccgggtc tttagcaatc accacgccag ccaccacgtc agagtgaccg
    28741 ttcagatatt tcgtacatga atgcaacacc agatcggcac ccaatgccag cggattttgt
    28801 aatgccgggc ttaagaaggt gttatccacc acgctcaccg ccccgacttc ccttgccaga
    28861 tggcagattt tcgcaatatc cacgacgcgt aacaatggat tacttgggct ttctaccagt
    28921 accagtttgg gtttttctgc cagcgctgcc cgtaatgcct gttcatcgcc ttgatcaaca
    28981 aacaacacgc gatagcaacc gcgtttcgcc agactgtcga acaggcgata gctaccgccg
    29041 tagcagtcgt gcggcgcaac cagcagatcg ccaggtttca aaaagacggt cgttaccagg
    29101 tgaatcgcgg acatgccggt attagtaagt actgcgccag caccgccttc cagttctgcc
    29161 agcgcacgct gaaccacatc gcgcgttggg ttgccgcgac gcgagtaatc atgcgcgcgc
    29221 ggttcattaa atccggtaaa gttataggtg ctggaaagat ggatgggagg gacaacgcaa
    29281 ccatactgtt cgtcgtcatt taacccgcta cgcactgcga tggtggcctg tttacgcgtc
    29341 atgtgatgaa gtttcctggg ctttgtcggt gaaatgtcag gcaccagagt aaacattgtg
    29401 ttaatggacg tcaatacatc tggacatcta aacttctttg cgtatagatt gagcaaatcc
    29461 caaatagccg ttaaaattat atgcattatc acgccgacag gtgcattaca cgatgtcacg
    29521 gtaacgcctg tacggtaaac tatgcgggtt tacggtcagt acccacataa attgtggggt
    29581 ctggtctcaa tttattgacg aagaggatta agtatctcat ggctgaatgg agcggcgaat
    29641 atatcagccc atacgctgag cacggcaaga agagtgaaca agtcaaaaag attacggttt
    29701 ccattcctct taaggtgtta aaaatcctca ccgatgaacg cacgcgtcgt caggtgaaca
    29761 acctgcgtca cgctaccaac agcgagctgc tgtgcgaagc gtttctgcat gcctttaccg
    29821 ggcaaccttt gccggatgat gccgatctgc gtaaagagcg cagcgacgaa atcccggaag
    29881 cggcaaaaga gatcatgcgt gagatgggga ttaacccgga gacgtgggaa tactaaagcg
    29941 gaaaagagcg gcgcggagtg gaatcgcctg atgcactacg cttatcaggc ctacgtcata
    30001 ttgcaattta ttgaatttgc acgaacttgt aggccggata aggcgttcac gccgcatccg
    30061 gcataaacaa cgagcacgtt gtctgcgacc caccgctttt tatacatgga cgtttaacta
    30121 tgaaaaacag gctgctgatc ctcagcctgc tggtttctgt acctgccttt gcctggcagc
    30181 cgcaaactgg cgacatcatc tttcaaatct ctcgctcatc gcaaagtaaa gcgatccaac
    30241 tggcaaccca ttccgattac agccacaccg gtatgctggt gatgcgtaac aaaaagccct
    30301 acgtttttga agcggtcggc ccggtgaaat acaccccgct caagcagtgg attgcccatg
    30361 gtgaaaaggg caactacgtt gttcgccgcg ttgaaggcgg actgagtgtt gaacaacagc
    30421 aaaaactgac gcaaacggca aaacgttatc tcggtaaacc gtatgatttc agcttctcat
    30481 ggagcgacga tcgccagtac tgttcggaag tggtgtggaa ggtttaccag aacgcgctgg
    30541 gaatgcgcgt gggcgaacag cagaagttga aagagtttga tctcagcaac ccgctggtcc
    30601 aggcgaagct caaagaacgc tacggtaaaa atattccgct tgaggaaacg gtagtttcac
    30661 cacaagccgt tttcgacgcg ccacaactca ccacggttgc caaagagtgg ccgctgtttt
    30721 cgtggtgata cgaccaaaat tcaggcacaa aaaaagcgcc gtgcggcgct tttttcggaa
    30781 atccggtctt atttgctgcc cgggatgttg aaacgcttgt tgaagcggtc aacacggcca
    30841 ccggtagcaa catcacgctg tttgccagtg aagaacgggt ggcacttgct gcacacgtcg
    30901 aggttcaggt catgaccaac ggtggagcgg atttttatta cgttaccgca agagcagcta
    30961 gcagtaattt cttcgtattt cgggtgaata tcttttttca tgggaaaacc tcggtttaag
    31021 gccgcgtcgc tcttccagcc ctaacgccag acaccacgcg atgttaaaag tatagcttca
    31081 atacgatcat ttcgtacgaa ggcgcgaaat catacagaaa ttaaccagcg tatgcaaact
    31141 gatccgcact cttctacggc aatgtgtata ctaacccacc gaatttcaag tcaggatgat
    31201 gctatgcccg ttgcccacgt tgccttgccc gttccgcttc ctcgtacctt tgactatctg
    31261 ctgccagaag gcatgacggt taaagctggg tgtcgcgtgc gcgtgccgtt tggcaaacag
    31321 caggagcgca tcgggattgt ggtatcagtt agcgatgcca gcgaactgcc gctcactgag
    31381 ctaaaagcgg tagtcgaagt gctggatggt gagccggtgt ttactcactc cgtctggcga
    31441 ttgctgctat gggcggcaga ttactatcat catccgattg gcgatgtgct gtttcatgcc
    31501 ttgccgattt tactacgcca ggggcggcct gcggcgaacg cgccgatgtg gtactggttt
    31561 gccactgaac aaggtcaggc ggtggatctg aacagcctga aacgctcacc caagcaacaa
    31621 caggcgctgg cagcgttacg gcaaggcaaa atctggcgcg accaggttgc cacgctcgaa
    31681 tttaatgatg ccgcgttgca ggcgctgcgc aaaaaaggtc tgtgtgattt agcaagtgaa
    31741 acaccagagt ttagcgactg gcgaacgaac tatgccgttt ctggtgagcg gttgcgattg
    31801 aataccgaac aggccaccgc cgttggcgca attcatagcg cggcagatac tttttctgcc
    31861 tggctgctgg cgggcgttac cggttccggt aaaacggagg tttatctcag tgtactggaa
    31921 aacgtgctcg ctcagggcaa acaggcgctg gtgatggtgc cggaaatcgg cctgacaccg
    31981 caaactatcg cccgttttcg tgaacggttt aatgcgcccg tggaagttct gcattccggt
    32041 ctgaacgaca gcgagcgtct ttcggcgtgg ctgaaagcga aaaatggcga ggcggcgatt
    32101 gtgatcggca cccgctccgc gctgtttacg ccgtttaaaa atctcggcgt gattgtcatt
    32161 gacgaagagc acgacagttc ctacaagcag caggaaggct ggcgctatca tgcccgcgac
    32221 ctggcggtgt atcgtgcgca tagcgagcaa atcccgatta ttcttggctc cgcaacgccc
    32281 gcgctggaaa cgttgtgcaa cgtccagcag aaaaaatacc gcctgctgcg cctgacgcgc
    32341 cgggcgggga atgcgcgtcc ggcaattcaa cacgtgctgg atttaaaagg tcagaaggtg
    32401 caggcaggtc tggctccggc gttaatcacc cgtatgcgcc agcatttaca ggccaacaat
    32461 caggttatcc tctttcttaa ccgccgtggc tttgcgcctg cgctactgtg ccacgactgc
    32521 ggctggattg ccgaatgccc acgttgcgat cactactaca cgctgcatca ggcgcaacag
    32581 catttgcgct gtcaccactg tgacagtcag cgtccggtgc cgcgccagtg cccttcctgc
    32641 ggttccacgc acctggtacc cgtggggctg ggcaccgaac agcttgaaca gacgcttgcg
    32701 ccgctgttcc ctgatgtgcc catttctcgt atcgaccgcg ataccaccag ccgcaaaggg
    32761 gcgctggaac agcaactggc ggaagtacat cgcggcggtg cgaggatttt gatcggcaca
    32821 caaatgctgg cgaaaggtca ccactttccg gatgtgacgc tggtggcgtt actggatgtg
    32881 gacggcgcgc tgttttctgc cgattttcgc tcggcagagc gtttcgctca gctttacacc
    32941 caggtcgctg gtcgtgccgg gcgtgcgggt aaacaaggcg aagtggtgct gcaaacgcac
    33001 catccggaac atcctctgtt gcaaacgttg ctctataaag gctacgatgc ctttgccgaa
    33061 caggcgctgg ctgagcgacg gatgatgcaa ctgccgccat ggaccagcca tgtgattgtg
    33121 cgtgcggaag atcataacaa tcagcatgcg ccgctgttcc tgcaacaact gcgtaatctg
    33181 atcctctcca gcccgctggc agacgacaaa ctgtgggttc tcggtccggt tccggcactg
    33241 gcacctaaac gtggcggtcg ctggcgctgg cagatattgt tgcagcaccc ttcccgcgtg
    33301 cgcttgcaac acatcattag tggtacgctg gcgctcatca atacaatacc ggattcccgc
    33361 aaggtgaaat gggtactgga tgttgatccg attgagggtt aaaccgctta cgatgcgagg
    33421 cggatcgaaa aattcaatat tcatcacact tttcatgaaa attctgtaac cgttttcacg
    33481 cgctatctgc taaaaatgtt gccgatgtga agtaaacatg gatgtagtac gcctgacgtg
    33541 ccaggcgagg agtgagtgtg aaagcgaaga agcaggaaac tgccgcgacc atgaaagacg
    33601 ttgccctcaa ggcaaaagtc tctacagcga ctgtctcccg agcattaatg aatcccgata
    33661 aagtctccca ggccacccgt aatcgggttg aaaaagcggc ccgggaagtg ggttatttac
    33721 cgcagcctat ggggcgcaac gtcaagcgta atgaatcccg caccattctg gtgattgtcc
    33781 cggatatctg cgatcccttc tttagcgaaa ttattcgcgg tatcgaagtt acggcggcaa
    33841 atcacggata tctggtgctg attggcgact gtgcgcatca aaatcagcag gaaaaaacct
    33901 ttatcgattt gatcatcacc aagcaaattg atggcatgtt gctgctgggt tcaaggctgc
    33961 cgtttgatgc cagcattgag gaacagcgta atctgccgcc gatggtgatg gcgaacgaat
    34021 ttgcaccgga actggagctg cctaccgttc atatcgacaa tctgaccgcc gcatttgatg
    34081 cagtaaatta tttatatgag caagggcata aacggattgg ctgtatagcc ggtcccgaag
    34141 agatgccact gtgtcactac cgcctgcaag gctatgttca ggcgctgcgt cgctgcggaa
    34201 ttatggttga tccacaatac atcgcccgtg gcgacttcac cttcgaagcc ggaagcaaag
    34261 caatgcaaca gctgcttgat cttccacaac cgcctactgc agtcttctgc catagcgatg
    34321 tgatggcgct cggcgcactt tctcaggcaa aacgccaggg gctgaaagtc ccggaagacc
    34381 tttccataat cggttttgat aacatcgacc tgacgcaatt ttgtgatccg ccgctgacaa
    34441 ccatcgcgca gccgcgttac gaaatcggtc gggaagctat gctgttattg cttgatcaaa
    34501 tgcaggggca acacgttggc agtggctctc gtttaatgga ctgcgaactt atcatccggg
    34561 gatcaacacg cgcgttacct taaagtaaac ctttaagact gccgtgactg gtcaaaggcc
    34621 cgccgcttaa gtaacatggc gggctgacga acgaataaat acagcgaaac gatagtggca
    34681 caacgagatt atgtacgccg cagccaaccg gcaccttcgc ggcgaaaaaa gagcacctca
    34741 cggaaaaagc aacgaaatct gcctgcggtt tctcccgcta tggtcgctat tgctgccgcc
    34801 gttcttgtga cctttatcgg tggtctgtac ttcattacgc atcacaagaa agaagagtcc
    34861 gagacgctgc aaagccagaa agtgaccgga aacggactac caccaaaacc agaagaacgc
    34921 tggcgctaca ttaaagagct ggaaagtcgc cagccgggtg tgcgtgcgcc cacagaacct
    34981 tctgccggtg gtgaagtgaa aacgccggag caactgacac cagaacaacg tcagcttctt
    35041 gaacaaatgc aggctgatat gcgccagcag ccaacgcagc tggttgaggt tccgtggaat
    35101 gaacagacgc cggaacagcg tcagcaaacg ctacaacgcc agcgtcaggc gcagcaatta
    35161 gcggagcagc aacgcctggc tcagcagtcg cgtacgactg aacaaagctg gcagcagcag
    35221 acgcgtacgt cgcaagccgc gccggtacag gcgcaaccgc gccagtctaa accagcttct
    35281 actcagcagc cgtaccagga tctgctgcaa acccctgcgc acacgaccgc gcagtcgaag
    35341 ccgcagcaag ctgcgccggt tgctcgtgcc gctgacgcgc caaaaccgac ggcggagaaa
    35401 aaagacgaac gccgctggat ggtgcagtgc ggttcgttca gaggcgcgga acaggcagag
    35461 acggtacgtg ctcagctggc gttcgaagga tttgactcga aaatcaccac caacaatggc
    35521 tggaatcgtg tggtcattgg cccggtgaaa ggcaaagaga acgcagacag caccctcaat
    35581 cggttgaaga tggcgggtca tacaaactgc attcggctcg ccgccggggg ttgaaaccct
    35641 caaaatcccc cccatctata attgcattat gccccgtact tttgtacggg gtttgtactc
    35701 tgtattcgta accaaggggt cagctcgtga caactatagt aagcgtacgc cgtaacggcc
    35761 atgtggtcat cgctggtgat ggtcaggcca cgttgggcaa taccgtaatg aaaggcaacg
    35821 tgaaaaaggt ccgccgtctg tacaacgaca aagtcatcgc gggctttgcg ggcggtactg
    35881 cggatgcttt tacgctgttc gaactgtttg aacgtaaact ggaaatgcat cagggccatc
    35941 tggttaaagc cgccgttgag ctggcaaaag actggcgtac cgatcgcatg ctgcgcaaac
    36001 ttgaagcact gctggcagtc gcggatgaaa ctgcatcgct tatcatcacc ggtaacggtg
    36061 acgtggtgca gccagaaaac gatcttattg ctatcggctc cggcggccct tacgcccagg
    36121 ctgcggcgcg cgcgctgtta gaaaacactg aacttagcgc ccgtgaaatt gctgaaaagg
    36181 cgttggatat tgcaggcgac atttgcatct ataccaacca tttccacacc atcgaagaat
    36241 taagctacaa agcgtaagga tctcccatgt ctgaaatgac cccacgcgaa atcgtcagcg
    36301 aactggataa gcacatcatc ggccaggaca acgccaagcg ttctgtggcg attgctctgc
    36361 gtaaccgctg gcgtcgcatg cagctcaacg aagagctgcg ccatgaagtg accccgaaaa
    36421 atatcctgat gatcggcccg accggtgtcg gtaaaactga aatcgcccgt cgtctggcta
    36481 agctggcgaa tgcgccgttc atcaaagttg aagcgaccaa atttaccgaa gtgggctacg
    36541 tcggtaagga agttgattct attattcgcg atctgaccga tgccgccgtg aaaatggtac
    36601 gcgtccaggc tatcgagaaa aaccgttatc gcgctgaaga actggcagaa gaacgtattc
    36661 tcgacgtgct gatcccacct gctaaaaaca actggggaca gactgaacag cagcaggaac
    36721 cttccgctgc tcgtcaggca ttccgcaaaa aactgcgtga aggccagctt gatgacaaag
    36781 aaatcgagat cgatcttgcc gcagcaccga tgggcgttga aattatggct cctccgggca
    36841 tggaagagat gaccagccag ctgcagtcca tgttccagaa cctgggcggc cagaagcaaa
    36901 aagcgcgtaa gctgaaaatc aaagacgcca tgaagctgct gattgaagaa gaagcggcga
    36961 aactggtgaa cccggaagag ctgaagcaag acgctatcga cgctgttgag cagcacggga
    37021 tcgtgtttat cgacgaaatc gacaaaatct gtaagcgcgg cgagtcttcc ggtccggatg
    37081 tttctcgtga aggcgttcag cgtgacctgc tgccgctggt agaaggttgc accgtttcca
    37141 ccaaacacgg gatggtcaaa actgaccaca ttctgtttat cgcttctggc gcgttccaga
    37201 ttgcgaaacc gtctgacctg atcccggaac tgcaaggtcg gctgccaatc cgcgttgaac
    37261 tgcaggcact gaccaccagc gacttcgagc gtattctgac cgagccgaat gcctctatca
    37321 ccgtgcagta caaagcactg atggcgactg aaggcgtaaa tatcgagttt accgactccg
    37381 gtattaaacg catcgcggaa gcggcatggc aggtgaacga atctaccgaa aacatcggtg
    37441 ctcgtcgttt acacactgtt ctggagcgtt taatggaaga gatttcctac gacgccagcg
    37501 atttaagcgg tcaaaatatc actattgacg cagattatgt gagcaaacat ctggatgcgt
    37561 tggtggcaga tgaagatctg agccgtttta tcctataatc gcgttcaatc attttcatca
    37621 ttgtttgatg gggctgaaag gccccatttt tattggcgcg tattatgact gaacaacaaa
    37681 ttagccgaac tcaggcgtgg ctggaaagtt tacgacctaa aaccctcccc ctcgcctttg
    37741 ctgcaattat cgtcgggacg gcgctggcat ggtggcaagg tcacttcgat ccgctggtcg
    37801 ccctgctggc actaattacc gccgggctat tacagatcct ttctaacctc gccaatgatt
    37861 acggcgatgc ggtaaaaggc agcgataaac ctgaccgcat tgggccgcta cgcggcatgc
    37921 aaaaaggggt cattacccag caaaagatga aacgggcgct cattattacc gtcgtgctca
    37981 tctgtctctc cgggctggca ctggttgcag tggcatgcca tacgctggcc gattttgtcg
    38041 gtttcctgat tcttggcggg ttgtcgatca ttgccgctat cacctacacc gtgggcaatc
    38101 gtccttatgg ttatatcggt ctgggtgata tttccgtact ggttttcttt ggctggttga
    38161 gtgtcatggg gagctggtat ttacaggctc atacattgat tccggcactg atccttccgg
    38221 cgaccgcatg cggcctgctg gcaacggcag tactgaatat taataacctg cgtgatatca
    38281 atagcgaccg cgaaaatggc aaaaacacgc tggtggtgcg cttaggtgaa gtgaacgcgc
    38341 gtcgttatca tgcctgcctg ctgatgggct cgctggtgtg tctggcgctg tttaatctct
    38401 tttcgctgca tagcctgtgg ggctggctgt tcctgctggc ggcaccatta ctggtgaagc
    38461 aagcccgtta tgtgatgcgg gaaatggacc cggtggcgat gcgaccaatg ctggaacgta
    38521 ctgtcaaggg agcgttactg actaacctgc tgtttgtttt agggatattc ctaagccagt
    38581 gggcagcata actgacaaat atcaattaac aattgatgat tttgccaaca gcccacatag
    38641 cgcgatatac tgaaaattct cgcagcaact gaatgttaag cctatgaaat acgatacttc
    38701 cgagctttgt gacatctatc aagaagatgt taacgtcgtg gaaccgctgt tctccaactt
    38761 tggcggacgg gcgtcgtttg gcggacaaat aatcacggta aaatgtttcg aggacaacgg
    38821 gttgctgtac gatctgctcg aacagaatgg ccgtggtcgt gttcttgtcg tcgatggcgg
    38881 tggttctgtt cgtcgcgcac tggtcgatgc tgaactggcg cgtctggcag tacaaaatga
    38941 atgggaaggt ctggtcattt acggcgcggt gcgtcaggta gatgacctgg aagagttgga
    39001 tatcggcatc caggcgatgg cggcaattcc ggttggtgcc gctggcgaag gcattggcga
    39061 aagcgatgtc cgcgtcaatt ttggcggtgt caccttcttc tccggcgacc atctttatgc
    39121 cgacaatacc gggattattc tttcagaaga tccgctggat attgaatgat aagaaaggca
    39181 ccgcaaggtg ccttttttct gcgttacctg ttggcctaca cagtaaagaa attacgcgaa
    39241 agatgaagcg taatcagacc tcttccatgc gacccagcag ggcctgcaga cgttcctgcc
    39301 agccgttctg ctgttctttc agatggttgt tctcacgctc cagctcttcg cgctgatgct
    39361 gggcattttg aacttcctgc gacagtgagt tgtttttttc tttcagctct tcgatttcca
    39421 tctgcaacag agtgatggta tcaatcgcct gctgtacttt tgcttccagt ttctcaaaca
    39481 cttctaatga cattgtcata cctctcctga attgcaaggc gttgatggat aaaaaatcct
    39541 cgtcccgatt accggtgacg ccttaataaa tacgagcgca ctttagttag ctccgattgt
    39601 atgaagccgc gccatcgctg tccagcggca cgccttgcag attacggttt gccacacttt
    39661 tcatccttct cctagtgaca taatccacat caatcgaaaa tgttaataaa tttgttgcgc
    39721 gaatgatcta acaaacatgc atcatgtaca atcagatgga ataaatggcg cgataacgct
    39781 cattttatga cgaagcacac acattttaag ttcgatattt ctcgtttttg ctcgttaacg
    39841 ataagttaac agcatgccta caagcatcgt ggaggtccgt gactttcacg catacaacaa
    39901 acattaactc ttcaggatcc gattatgagt caaacatcaa ccttgaaagg ccagtgcatt
    39961 gctgaattcc tcggtaccgg gttgttgatt tttttcggtg tgggttgcgt tgcagcacta
    40021 aaagtcgctg gtgcgtcttt tggtcagtgg gaaatcagtg tcatttgggg actgggggtg
    40081 gcaatggcca tctacctgac cgcaggggtt tccggcgcgc atcttaatcc cgctgttacc
    40141 attgcattgt ggctgtttgc ctgtttcgac aagcgcaaag ttattccttt tatcgtttca
    40201 caagttgccg gcgctttctg cgctgcggct ttagtttacg ggctttacta caatttattt
    40261 ttcgacttcg agcagactca tcacattgtt cgcggcagcg ttgaaagtgt tgatctggct
    40321 ggcactttct ctacttaccc taatcctcat atcaattttg tgcaggcttt cgcagttgag
    40381 atggtgatta ccgctattct gatggggctg atcctggcgt taacggacga tggcaacggt
    40441 gtaccacgcg gccctttggc tcccttgctg attggtctac tgattgcggt cattggcgca
    40501 tctatgggcc cattgacagg ttttgccatg aacccagcgc gtgacttcgg tccgaaagtc
    40561 tttgcctagc tggcgggctg gggcaatgtc gcctttaccg gcggcagaga cattccttac
    40621 ttcctggtgc cgcttttcag ccctatcgtt ggcgcgattg taggtgcatt tgcctaccgc
    40681 aaactgattg gtcgccattt gccttgcgat atctgtgttg tggaagaaaa ggaaaccaca
    40741 actccttcag aacaaaaagc ttcgctgtaa tatgactacg ggacaattaa acatgactga
    40801 aaaaaatata tcgttgcgct cgaccagggc accaccagct cccgcgcggt cgtaatggat
    40861 cacgatgcca atatcattag cgtgtcgcag cgcgaatttg agcaaatcta cccaaaacca
    40921 ggttgggtag aacacgaccc aatggaaatc tgggccaccc aaagctccac gctggtagaa
    40981 gtgctggcga aagccgatat cagttccgat caaattgcag ctatcggtat tacgaaccag
    41041 cgtgaaacca ctattgtctg ggaaaaagaa accggcaagc ctatctataa cgccattgtc
    41101 tggcagtgcc gtcgtaccgc agaaatctgc gagcatttaa aacgtgacgg tttagaagat
    41161 tatatccgca gcaataccgg tctggtgatt gacccgtact tttctggcac caaagtgaag
    41221 tggatcctcg accatgtgga aggctctcac gagcgtgcac gtcgtggtga attgctgttt
    41281 ggtacggttg atacgtggct tatctggaaa atgactcagg gccgtgtcca tgtgaccgat
    41341 tacaccaacg cctctcgtac catgttgttc aacatccata ccctggactg ggacgacaaa
    41401 atgctggaag tgctggatat tccgcgcgag atgctgccag aagtgcgtcg ttcttccgaa
    41461 gtatacggtc agactaacat tggcggcaaa ggcggcacgc gtattccaat ctccgggatc
    41521 gccggtgacc agcaggccgc gctgtttggt cagttgtgcg tgaaagaagg gatggcgaag
    41581 aacacctatg gcactggctg ctttatgctg atgaacactg gcgagaaagc ggtgaaatca
    41641 gaaaacggcc tgctgaccac catcgcctgc ggcccgactg gcgaagtgaa ctatgcgttg
    41701 gaaggtgcgg tgtttatggc aggcgcatcc attcagtggc tgcgcgatga gatgaagttg
    41761 attaacgacg cctacgattc cgaatatttc gccaccaaag tgcaaaacac caatggtgtg
    41821 tatgtggttc cggcatttac cgggctgggt gcgccgtact gggacccgta tgcgcgcggg
    41881 gcgattttcg gtctgactcg tggggtgaac gctaaccaca ttatacgcgc gacgctggag
    41941 tctattgctt atcagacgcg tgacgtgctg gaagcgatgc aggccgactc tggtatccgt
    42001 ctgcacgccc tgcgcgtgga tggtggcgca gtagcaaaca atttcctgat gcagttccag
    42061 tccgatattc tcggcacccg cgttgagcgc ccggaagtgc gcgaagtcac cgcattgggt
    42121 gcggcctatc tcgcaggcct ggcggttggc ttctggcaga acctcgacga gctgcaagag
    42181 aaagcggtga ttgagcgcga gttccgtcca ggcatcgaaa ccactgagcg taattaccgt
    42241 tacgcaggct ggaaaaaagc ggtcaaacgc gcgatggcgt gggaagaaca cgacgagtaa
    42301 tgtcaggcac ctgaatcgat tgcctgatgc tgatcgcctg atgcgacgct tacgcgtctt
    42361 atcaggccta cgagccaatg ccattttgtg taggcgggat aaggcgttca cgccgcatcc
    42421 ggcatcacgc agactctgaa accgtgcggg ggcaaccccg cacacatcaa taatcccacc
    42481 cttcccctgt gctacacttc gcgccattcc ttactgctta gagtttgcta tgagacgaga
    42541 acttgccatc gaattttccc gcgtcaccga atctgcggcg ctggctggct acaaatggtt
    42601 aggacgcggc gataaaaaca ccgcggacgg cgcggcggta aacgccatgc gtattatgct
    42661 caaccaggtc aacattgacg gcaccatcgt cattggtgaa ggtgaaatcg acgaagcacc
    42721 gatgctctac attggtgaaa aagtcggtac tggtcgcggc gacgcggtag atattgctgt
    42781 tgatccgatt gaaggcacgc gcatgacggc gatgggccag gctaacgcgc tggcggtgct
    42841 ggcagtaggc gataaaggct gcttcctcaa tgcgccggat atgtatatgg agaagctgat
    42901 tgtcgggccg ggagccaaag gcaccattga tctgaacctg ccgctggcgg ataacctgcg
    42961 caatgtagcg gcggcgctcg gcaaaccgtt gagcgaactg acggtaacga ttctggctaa
    43021 accacgccac gatgccgtta tcgctgaaat gcagcaactc ggcgtacgcg tatttgctat
    43081 tccggacggc gacgttgcgg cctcaattct cacctgtatg ccagacagcg aagttgacgt
    43141 gctgtacggt attggtggcg cgccggaagg cgtggtttct gcggcggtga tccgcgcatt
    43201 agatggcgac atgaacggtc gtctgctggc gcgtcatgac gtcaaaggcg acaacgaaga
    43261 gaatcgtcgc attggcgagc aggagctggc acgctgcaaa gcgatgggca tcgaagccgg
    43321 taaagtattg cgtctgggcg atatggcgcg cagcgataac gtcatcttct ctgccaccgg
    43381 tattaccaaa ggcgatctgc tggaaggtat tagccgcaaa ggcaatatcg cgactaccga
    43441 aacgctgctg atccgcggca agtcacgcac cattcgccgc attcagtcca tccactatct
    43501 ggatcgcaaa gacccggaaa tgcaggtgca cattctctga gtgatttgat cgattgagcc
    43561 ttccagtcct tcgggactgg aatttttttg ttcggagaac gaagataagg caagtcaatc
    43621 aaaacaggag aaaaacatgg ctgattgggt aacaggcaaa gtcactaaag tgcagaactg
    43681 gaccgacgcc ctgtttagtc tcaccgttca cgcccccgtg cttccgttta ccgccgggca
    43741 atttaccaag cttggccttg aaatcgacgg cgaacgcgtc cagcgcgcct actcctatgt
    43801 taactcgccc gataatcccg atctggagtt ttacctggtc accgtccccg atggcaaatt
    43861 aagcccacga ttggcggcac tgaaaccggg cgatgaagtg caggtggtta gcgaagcagc
    43921 tggcttcttt gttctggatg aagtaccgga ctgcgaaacg ctatggatgc tggcaaccgg
    43981 tacagcgatt ggcccttatt tatcgattct gcaactaggc aaagatttag atcgcttcaa
    44041 aaatctggtc ctggttcacg ccgcacgtta tgccgccgac ttaagctatt tgccactgat
    44101 gcaagaactg gaaaaacgct acgaaggcaa actgcgcatt cagacggtgg tcagtcggga
    44161 aacggcagcg gggtcgctca ccggacggat accggcatta attgaaagtg gggaactgga
    44221 aagcgcgatt ggcctgccga tgaataaaga aaccagccat gtgatgctgt gcggcaatcc
    44281 acagatggtg cgcgatacac aacagttgct gaaagagacc cggcagatga cgaaacattt
    44341 acgtcgccga ccgggccata tgacagcgga gcattactgg taagcggtta cttatcgata
    44401 aacggcacga tgagcaaatc cgcactcatc ttattgatca tcccgcgata tgccggcatc
    44461 aaacggttga taaatgagtg atgatgacca cagacaagga ggtcgcactg ctctttttgc
    44521 ataatttcca gcagtgtttc cggcatttct ccgcgttcaa tacgcagttt tgtcttcggc
    44581 cattgaatat ttttcgtcag tttatacagc ttgttatccg acttattctt caacaattga
    44641 agaatatctt ctgttgcagg gaagtagata cccggataca actcgcttaa gccatcatca
    44701 atatgaatta acgtcaggtg agcgtcatta tgtctggcga gctccagggc tttattcacc
    44761 agtaaggcat cttcttcatt cccggaaatt gccacgccaa tgtgtttata agccatgttt
    44821 aactccttct aaagcaactc catcaagcta acgaacgcag tgataactca aaaataatca
    44881 tctcggcctg gcatgagaaa gtgaaggccg catcaagctc aacttgcccc tccagatcct
    44941 taatcttata ggccacgctg gccccttcat ctgcagagga tgcagcggcg gcaagctctt
    45001 tcagtttggt cataaaagac tctgcttctt cgcgggtggc aaaaaagcgc gaaaacttac
    45061 tggtacagtt gtcgttatca atcaccgtac cgatatcaat cgcacatcct ttagtactgc
    45121 atttatctac gacatctttc atagggcacc tctgtatatt cgcctctctg tcgattacga
    45181 cttaagaata atgcgccgtt aagggaagaa caaacaagta atgatcttat ggattaccat
    45241 tttttttgat attgcttaag gtgaagagtt cagaaggatt ttccgggtat tgtcatttgc
    45301 atcgtggaaa taacaatccc ggaaaaaaat agcagtaaga gatatattaa aggttaatta
    45361 cttgatttat tgtcggcttt atacttcaca tcctgagtat ctttaccata tttattttcg
    45421 ccttgtgtgc caacaaacgc accaagatcg ataagcatca tcaccagaat caacgtcggg
    45481 acaaaacgcc ccaccgccca ttgccagaca cccggtaaaa tcgctcagtt acccgccagc
    45541 agcatccacg ccacaatcat cagaaatgcc catgcgccgg aacgcccgcg atcatgcaag
    45601 cgcttaacag ttactgccgc tgttggccag agcaagcaca caaggcaaaa cgccgcggtc
    45661 tgaatatcga gtagattctt acccgccagt gagaaaagca ccagcatgcc tgcgaaccac
    45721 aggcctatcc aaatccagaa atcacggcgt ccaatacgcc ctttaaatga gaataaccat
    45781 tgctgtatgg tcatgtaagt tccttgatgg ttgtcttttc caggattcta cccttttgac
    45841 aagggcgaca gatatcgttt taatcggagc cagtcataac aaaaggtact gtcaatgaag
    45901 ccagggtgta cgctgttttt tctcttatgt tctgcattaa ccgttacaac aacggcgcat
    45961 gcacaaacac cagatacggc aacgaccgcg ccttatctgc tggctggagc ccctactttc
    46021 gatctctcca tcagccagtt tcgagaagac tttaacagcc agaatcccag cctgccactg
    46081 aacgaatttc gtgccatcga cagcagtccc gacaaagcca atctcactcg tgctgcaagt
    46141 aaaattaacg agaacttgta tgcttctaca gcgctggagc gcggtacctt aaaaatcaaa
    46201 agcattcaaa tgacctggct acccatccag gggccagagc aaaaagcagc gaaagcgaaa
    46261 gctcaggaat acatggcagc agtgatccgc acactcaccc cattaatgac caaaacacaa
    46321 agccagaaaa aactgcagtc gctactaacg gcggggaaaa acaaacgtta ttacaccgag
    46381 acagaaggtg cactgcgtta tgttgtcgcg gacaacggcg aaaaggggct gaccttcgct
    46441 gttgaaccga ttaagctggc gctatctgaa tcgcttgaag gtttgaataa atgacaaaaa
    46501 gcaaagcctt tgtgccgatg aatctctata ctgtttcaca gacctgctgc cctgcggggc
    46561 ggccatcttc ctttattcgc ttataagcgt ggagaattaa aatgcgacat cctttagtga
    46621 tgggtaactg gaaactgaac ggcagccgcc acatggttca cgagctggtt tctaacctgc
    46681 gtaaagagct ggcaggtgtt gctggctgtg cggttgcaat cgcaccaccg gaaatgtaca
    46741 tcgatatggc gaagcgcgaa gctgaaggca gccacatcat gctgggtgcg caaaacgtgg
    46801 acctgaacct gtccggcgca ttcaccggtg aaacttctgc cgctatgctg aaagacatcg
    46861 gcgctcagta catcatcatc ggccactctg aacgtcgtac ttaccacaaa gagtctgacg
    46921 aactgattgc gaaaaaattc gcggtgctga aagagcaggg tctgactccg gttctgtgca
    46981 tcggtgaaac cgaagctgaa aacgaagcgg gaaaaactga agaagtttgc gcacgtcaga
    47041 tcgacgcggt actgaaaact cagggtgctg cggcattcga aggtgcggtt atcgcttacg
    47101 aacctgtatg ggcaatcggt actggcaaat ctgcaactcc ggctcaggca caggctgttc
    47161 acaaattcat ccgtgaccac atcgctaaag ttgacgctaa catcgctgaa caagtgatca
    47221 ttcagtacgg cggctctgta aacgcgtcta acgctgcaga actgtttgct cagccagaca
    47281 tcgacggcgc gctggttggc ggtgcttctc tgaaagctga cgctttcgca gtgatcgtta
    47341 aagctgcaga agcggctaaa caggcttaag tctgacaggt gccggatttc atatccggca
    47401 cttactttcc ttaactcctc gccttaacgc aaaatctcac actcatgatc ctggatttcc
    47461 tcggctgaag cacggttcag cgtcagtaga tttcgttgtg tcgccagcaa cacaaacgaa
    47521 ttatcactct gccgtaccat cgccagcccg tagcttccca tatgttcccg cgcctcaggc
    47581 acttcttctg caagcatcat aaatgggctg cgttgtacca gttcgctttc cgttacccga
    47641 cgcgccaggt attcatgccc gcgcaaacca cctggcagtg gcaaccagcg gctgctgatg
    47701 ttcgccagat ttttatccag ttgttcgcgc acatcaggac gaatacaaga gatatgaata
    47761 tgaaaatggt tttgcgtatg cccggtgcgg gagttgatcg ccaaagaaac cgcgcgatcg
    47821 ggaaccggct ggccgtattt tttgctcata aaatcacgcg cctgccaggc caaccaaaag
    47881 aagttcggcg ttgaaggatc ggtcaacaaa ggactttcag taccgttaat acgatacgtt
    47941 ggcatcaaca gatattgcag tggaccatta agatctttta aaaccacgta tccggcattg
    48001 ggtttgactt ccgcacatgg cgaaggattt tgattttgct gctgattggg caaacattcc
    48061 tcaaggacaa tcttacgtaa tgtatccgac tcttcaccgg ttaatttcca gtaaccaata
    48121 ccggcagcca caacggcgat aactatcatc accaaaaaaa gaagacccgc ttttttcatc
    48181 tttttttccc tgtacctcaa agagatgcaa gggtaacgca aaatcgtgac aaataaaaaa
    48241 cccggtcgcg agaaaccacc gggtttgata attatcctag ggaaatcagc gtttgctgat
    48301 ctgatcgaac gtaccgccgt tagcaaaatg ctctttttgc gctttcgtcc agccgccgaa
    48361 ctcttcatca atggtgaata acttcagctt tggaaacgca ttttcgtact ttttcgctac
    48421 ttcagcgtcg cgcggacggt agtagttttt cgcggcaatt tcctgacctt ctggcgagta
    48481 gagatatttc aggtaggctt ccgccacctc tttggtgcct tttttctcga ccactttatc
    48541 gaccaccgac accgttggct ctgcgaggat agactcactc ggcgtgacga tttcgaattt
    48601 atctttcccc agttcattcg ctgccagcag agcttcgttt tcccaggcga tcagtacatc
    48661 gccaatcccg cgctcgacaa aggtgttagt ggagccgcgc gcgccagaat ccagaacttc
    48721 gacgttttta tacagtgccc gaacaaaatc ctgtgctttt gcctgatcgt tgttgttgtg
    48781 atgcagcgcg tagccccagg cagccaggta gttccagcgt gcgccaccag agcttttcgg
    48841 attaggcgtg atcaccgaaa cacccggttt aatcagatcg ttccagtcat ggatctgctt
    48901 cggattgccc ttacgcacca ggaaaacaat ggtggaagtg tacggtgcgg agttatccgg
    48961 cagacgtttg atccactctt tatcaatccg cccgcgttcc gcaatcgcgt ccacgtcata
    49021 ggccagagcc agcgtgacaa catcagcttc aataccgttg attaccgacg tcgcttgttt
    49081 acctgaacca ccgtgtgact gacgaatcac cacgttatca ccagtttgct gtttccagtg
    49141 ggcgctgaat gccttgttgt actgttcgta caattcgcgc gttggatcat atgaaacgtt
    49201 aagaagctga atatcctttg ccataacgct ggttgccgcc agcaaaaatg ttaaccctac
    49261 gccccacttg ttcatcgccc gactctctta tgttgtgttg tgatgagcaa agcgtgccag
    49321 aaggttaacc aaacattaaa gaataaaaaa agattggcta taacttgcgg gtatatgttg
    49381 agggattaaa aaggcggaag aaatccgcct catattgctg acaaagtgcg ctttgtccat
    49441 gccggatgcg gcgcgaacgc cttatccggc ctacaaaagt ttgcaaattc aataaattgc
    49501 agaattcatg taggcctgat aagcgaagcg catcaggcat ttttgcttct gtcatcggtt
    49561 tcaggctaaa ggaatctgcc tttttccgaa atcattaata cagttttttc gcgcagtcca
    49621 gccagtcacc tttgaacgga cgcttcatgt tttcgatagc gtcgatgatg tcgtggtgaa
    49681 ccagctgttc gttctggata cctacacaac gaccgccgta acctgccagc agcagatcga
    49741 tagcgtaagc gcccatacgg gaagccagaa tacggtcgta aggcaccgga gaaccaccgc
    49801 gctggatgtg gcccagcaca gttgcgcggg tttcacgacc ggtttctttc tcgatgaaat
    49861 gcgccagttc gtcaacatca cacatatgtt cggtaatcgc cacgatcgcg tgttttttac
    49921 ctttcgcgat acccgctttg atttcgttta ccaggtcttc acggctgaat tcaacttccg
    49981 gaactacaac gaattcacag ccaccggcaa tggccgcagc cagggtcaga tcgccacaat
    50041 aacggcccat cacttccacc acggaaatac gctggtgaga agaagaggtg tcacgcagac
    50101 ggtcgatcgc ttctacaacg gtgctcagcg cagtgaagaa accgatagtg tagtcagtgc
    50161 ctttgatgtc gttgtcgata gtgcccggca gaccgatgca cgggaagccc atttcggtca
    50221 gacgcattgc acccatgtag gaaccgtcac cgccgataac caccagcgcg tcgataccac
    50281 gttttttcag gttttcgata gccacggcgc ggatgttctc gtcgcggaat tccgggaaac
    50341 gcgcagaacc gaggaacgta ccgccacggt tgatcatgtc agacacgctg taacggtcta
    50401 gctgtaccat acggtcttca tacagaccca gatagccgtc ataaataccc attacttcca
    50461 gaccttctgt cagcgcagaa cgaacaaccc cgcgaattgc ggcgttcatg cctggcgcat
    50521 caccgccgct tgtcaacaca cctattttct taatcatgac tacctctgaa ctttggaatg
    50581 caaaatgaaa tctgttgccg gaagtcttct tgcacatcga agtgatccaa cgaatgtgca
    50641 aatagtataa caatcacttc ctgctgaatt gattcaggtc aggccaaatg gcggtatttt
    50701 atacacaaaa tgcgggtctg gctctctttt atactgatta tgaaagcata gaccgtttac
    50761 cctccctggg tacgacggaa caggggtcct gatggataat cacatccgat cccggaaaac
    50821 gccgtaaaat agcctgctct acctgatccg ccaccatatg tgcctgaacc aaaggcagag
    50881 agtcttccat ttccaaatga atctgaataa agcgggtcgg ccctgactgc cgcgtgcgaa
    50941 gatcgtgagc gccgctaaca cccggccagg aagtcacgat atcaataatt tcttgccgtt
    51001 cttcatcagg caatgcgcga tccagtaatg actgtaccgc ctcatatccc atgcgtaacg
    51061 cgctatataa aatatagatg ccgattccca atgcaaacag agcatcggcg cgatgccagc
    51121 cgtaccaaga caaccccagc gccagcagaa ttgcgccgtt catcataaca tcagactggt
    51181 aatgtagcat atcagcccgc accgcctggc tttgcgtccg gcgcaccacc caccgctgaa
    51241 acgagacaag gataatcgta caaattagcg ccacaattgt cacgataacc ccgacgcctg
    51301 gatctgtcat cggtgttgga gataccagat gttgaatacc cgtcaaaaac aggaatagtg
    51361 ccgaaccgga gataaacata ctttgcgcca gcgccgcgag ggactcagct ttaccgtgac
    51421 caaacgagtg attatcgtcg gcaggttgca gggaatatcg caccaccagt aaattcgtca
    51481 acgacgcgcc gatatccacc agcgaatcca ccagcgcggc gagaatactc accgacccgg
    51541 tataccacca tgcaaaaatt ttaatcagca atagcaacga agccatcgcc gtcgcagcaa
    51601 tcgccgcccg actgaccagc cgtccataag attgattcat aaatactccc gctatcaact
    51661 gacgctagta taacggaagc aaatcatctg caatgcatta agcagcaggc aaattgagga
    51721 taaaaaaaac cccacatcat gtgggggaag acagggatgg tgtctatggc aaggaaaaca
    51781 gggtttacta ctgggaacgt gagttgctac tactcaatag cttcaacgat gaactttttt
    51841 gccattgcgt cacgtcacgc aactgctcca ttcgttgttg atgtttctcg tttaaaaccg
    51901 cttgctgctc cggcgttaac aggcgataca tttggttgcg gacttttgcc atctcaacct
    51961 gacgagcaat ttgctcattc gccatttttt ctgcctgtgc gcgcacagcg ttttcatcaa
    52021 aattttctgc ggtgacaagg cgatgcattg tctccagttc gctaacatta acaggaggct
    52081 gttcgtgccg ggcctgttgc ataagatctc gcatctgctg acgctgatgt tcggttaaac
    52141 ttatgccgtc gaacatatgg ctctgcgtac tgcgctgcgt aagttcttca cccggatgcc
    52201 agttatcgcc tgaaccgact tcagcagcgt ggcttaatga actgactgcc agcgttgagg
    52261 ccatgacggc agcggtaact atgcgcatca tttgctccca aaatctttct gtcgcgattc
    52321 aacgatagag agtttacgat tcaggctgca aacatgcgtc agggggtgta aaacaacgta
    52381 aagtcatgga ttagcgacgt ctgatgacgt aatttctgcc tcggaggtat ttaaacaatg
    52441 aataaaatcc tgttagttga tgatgaccga gagctgactt ccctattaaa ggagctgctc
    52501 gagatggaag gcttcaacgt gattgttgcc cacgatgggg aacaggcgct tgatcttctg
    52561 gacgacagca ttgatttact tttgcttgac gtaatgatgc cgaagaaaaa tggtatcgac
    52621 acattaaaag cacttcgcca gacacaccag acgcctgtca ttatgttgac ggcgcgcggc
    52681 agtgaacttg atcgcgttct cggccttgag ctgggcgcag atgactatct cccgaaaccg
    52741 tttaatgatc gtgagctggt ggcacgtatt cgcgcaatcc tgcgccgttc gcactggagc
    52801 gagcaacagc aaaacaacga caacggttca ccgacactgg aagttgatgc cttagtgctg
    52861 aatccaggcc gtcaggaagc cagcttcgac gggcaaacgc tggagttaac cggtactgag
    52921 tttaccctgc tctatttgct ggcacagcat ctgggtcagg tggtttcccg tgaacattta
    52981 agccaggaag tgttgggcaa acgcctgacg cctttcgacc gcgctattga tatgcacatt
    53041 tccaacctgc gtcgtaaact gccggatcgt aaagatggtc acccgtggtt taaaaccttg
    53101 cgtggtcgcg gctatctgat ggtttctgct tcatgatagg cagcttaacc gcgcgcatct
    53161 tcgccatctt ctggctgacg ctggtgctgg tgttgatgtt ggttttgatg ttacccaagc
    53221 tcgattcacg ccagatgacc gagcttctgg atagcgaaca gcgtcagggg ctgatgattg
    53281 agcagcatgt cgaagcggag ctggcgaacg atccgcccaa cgatttaatg tggtggcggc
    53341 gtctgttccg ggcgattgat aagtgggcac cgccaggaca gcgtttgtta ttggtgacca
    53401 ccgaaggccg cgtgatcggc gctgaacgca gcgaaatgca gatcattcgt aactttattg
    53461 gtcaggccga taacgccgat catccgcaga agaaaaagta tggccgcgtg gaactggtcg
    53521 gtccgttctc cgtgcgtgat ggcgaagata attaccaact ttatctgatt cgtccggcca
    53581 gtagttctca atccgatttc attaacttat tgtttgaccg cccgctatta ctgctgattg
    53641 tcaccatgtt ggtcagtacg ccgctgctgt tgtggttggc ctggagtctg gcaaaaccgg
    53701 cgcgtaagct gaaaaacgct gccgatgaag ttgcccaggg aaacttacgc cagcacccgg
    53761 aactggaagc ggggccacag gaattccttg ccgcaggtgc cagttttaac cagatggtca
    53821 ccgcgctgga gcgcatgatg acctctcagc agcgtctgct ttctgatatc tctcacgagc
    53881 tgcgcacccc gctgacgcgt ctgcaactgg gtacggcgtt actgcgccgt cgtagcggtg
    53941 aaagcaagga actcgagcgt attgaaaccg aagcgcaacg tctggacagc atgatcaacg
    54001 atctgttggt gatgtcacgt aatcagcaaa aaaacgcgct ggttagcgaa accatcaaag
    54061 ccaaccagtt gtggagtgaa gtgctggata acgcggcgtt cgaagccgag caaatgggca
    54121 agtcgttgac agttaacttc ccgcctgggc cgtggccgct gtacggcaat ccgaacgccc
    54181 tggaaagtgc gctggaaaac attgttcgta atgctctgcg ttattcccat acgaagattg
    54241 aagtgggctt tgcggtagat aaagacggta tcaccattac ggtggacgac gatggtcctg
    54301 gcgttagccc ggaagatcgc gaacagattt tccgtccgtt ctatcggacc gatgaagcac
    54361 gcgatcgtga atctggcggt acaggattgg ggctggcgat tgttgaaacc gccattcagc
    54421 agcatcgtgg ctgggtgaag gcagaagaca gcccgctggg cggtttacgg ctggtgattt
    54481 ggttgccgct gtataagcgg agttaaactc cgcatttgta ggcaggataa ggcgtttacg
    54541 ccgcatccgg catttgagca ggatgcctga tgcgacgctg atagcgtctt atcaggccta
    54601 cactccgggg gttattttcc ccacaaccgg cgggaaaaat cttcgatctt gccgctcagt
    54661 cggcgctttt gcatcgtccg cgtccagctt ttcgataacc cggcggcgga gagtaaacgg
    54721 tgatactgat cgtcatcaaa cggcatatgc catgcgatgg cggcagcctc ctgcacggtc
    54781 acatcgctga cacgggaaac caactccagc ggcgcgtccg cagacacttt ccctggcgct
    54841 atcacgctat acaaccaccc caccttacct gtgttttgca tcaactgcgc tatatcgctg
    54901 atatcaaaat ggtaattgag tttatagcaa ggcgagcgcg gctggctgac ctgaattaat
    54961 gcctctcccc agcggaaaat atcgcccata tgaacattac tttccgtgag accgtcggtt
    55021 gagagatttt cgccaaacgc aggcgcaaca aacaactccg cctgttccgg aaattcccgc
    55081 acccagtaga gatagtgctc acgaggataa tgacacagcg ctctgtctgg cccgccgtga
    55141 acctttttct ccgcctgctc gtcaccttcc agccccagct ccgtcagcat caactcacca
    55201 tcaacctgga ttttggcaat tgcgctgggt ttgccttcgg gataagcctg aatcttgcct
    55261 gtgtatacat caaccggata tcgcatctgt tccctctcca cgagaaattt ccccattaga
    55321 ccatatttat tttatgtcag ccgtgctgtt ttcgcggtaa gtatccaggc aggaactcaa
    55381 tagcaaaccg gacgaacgtt agttcatccg gctgtggg
//
LOCUS       DAZPCS010000010        54722 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZPCS010000010 DAZPCS010000000
VERSION     DAZPCS010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 54722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54722
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.010"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(354..1727)
                     /gene="eutA"
                     /locus_tag="ACHUQ7_000562"
     CDS             complement(354..1727)
                     /gene="eutA"
                     /locus_tag="ACHUQ7_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFR1134117.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANYALFDAGKISG
                     TACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQLVQVTRRMAAL
                     IVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPANPFCFADIGPLL
                     ATALHDHPRLREMNVQFPAQTVRSTVIGAGAHTLSLSGSTIWLEGVQLPLRNLPVAIP
                     IDETDLVSAWQQALLQLDLDPKTDVYVLALPASLPVRYAAVLTVINALVDFVARFPNP
                     HPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPLFGGSVVPVTV
                     KSLAFPS"
     gene            complement(1702..2181)
                     /gene="eutP"
                     /locus_tag="ACHUQ7_000563"
     CDS             complement(1702..2181)
                     /gene="eutP"
                     /locus_tag="ACHUQ7_000563"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFR1134118.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTLAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(2194..2529)
                     /gene="eutS"
                     /locus_tag="ACHUQ7_000564"
     CDS             complement(2194..2529)
                     /gene="eutS"
                     /locus_tag="ACHUQ7_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFR1134119.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(2822..5101)
                     /gene="maeB"
                     /locus_tag="ACHUQ7_000565"
     CDS             complement(2822..5101)
                     /gene="maeB"
                     /locus_tag="ACHUQ7_000565"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFR1134120.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDSLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            5390..6340
                     /gene="tal"
                     /locus_tag="ACHUQ7_000566"
     CDS             5390..6340
                     /gene="tal"
                     /locus_tag="ACHUQ7_000566"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFR1134121.1"
                     /translation="MNELDGIKQFTTVVADSCDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGCVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     TQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            6360..8363
                     /gene="tkt"
                     /locus_tag="ACHUQ7_000567"
     CDS             6360..8363
                     /gene="tkt"
                     /locus_tag="ACHUQ7_000567"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFR1134122.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFPRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQMEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(8466..9509)
                     /locus_tag="ACHUQ7_000568"
     CDS             complement(8466..9509)
                     /locus_tag="ACHUQ7_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFR1134123.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDARPTLWITR"
     gene            complement(9635..10210)
                     /gene="nudK"
                     /locus_tag="ACHUQ7_000569"
     CDS             complement(9635..10210)
                     /gene="nudK"
                     /locus_tag="ACHUQ7_000569"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFR1134124.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(10278..12257)
                     /gene="aegA"
                     /locus_tag="ACHUQ7_000570"
     CDS             complement(10278..12257)
                     /gene="aegA"
                     /locus_tag="ACHUQ7_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFR1134125.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKFGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDLLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            12463..14180
                     /gene="narQ"
                     /locus_tag="ACHUQ7_000571"
                     /pseudo
     CDS             12463..14180
                     /gene="narQ"
                     /locus_tag="ACHUQ7_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
     gene            14382..17495
                     /gene="acrD"
                     /locus_tag="ACHUQ7_000572"
     CDS             14382..17495
                     /gene="acrD"
                     /locus_tag="ACHUQ7_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFR1134126.1"
                     /translation="MANFFIDRPIFAWVLTILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASSANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMLLTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(17594..17653)
                     /gene="ypfM"
                     /locus_tag="ACHUQ7_000573"
     CDS             complement(17594..17653)
                     /gene="ypfM"
                     /locus_tag="ACHUQ7_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFR1134127.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            18034..18390
                     /locus_tag="ACHUQ7_000574"
     CDS             18034..18390
                     /locus_tag="ACHUQ7_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFR1134128.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            18394..19521
                     /gene="dapE"
                     /locus_tag="ACHUQ7_000575"
     CDS             18394..19521
                     /gene="dapE"
                     /locus_tag="ACHUQ7_000575"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFR1134129.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            19549..19749
                     /locus_tag="ACHUQ7_000576"
     CDS             19549..19749
                     /locus_tag="ACHUQ7_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFR1134130.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(19859..20557)
                     /gene="ypfH"
                     /locus_tag="ACHUQ7_000577"
     CDS             complement(19859..20557)
                     /gene="ypfH"
                     /locus_tag="ACHUQ7_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR1134131.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDQAL
                     SGGKPGDDDVIEMM"
     gene            complement(20631..22646)
                     /gene="tmcA"
                     /locus_tag="ACHUQ7_000578"
     CDS             complement(20631..22646)
                     /gene="tmcA"
                     /locus_tag="ACHUQ7_000578"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFR1134132.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            complement(22661..23524)
                     /locus_tag="ACHUQ7_000579"
     CDS             complement(22661..23524)
                     /locus_tag="ACHUQ7_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFR1134133.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVIQATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            complement(23692..24405)
                     /gene="purC"
                     /locus_tag="ACHUQ7_000580"
     CDS             complement(23692..24405)
                     /gene="purC"
                     /locus_tag="ACHUQ7_000580"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFR1134134.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            complement(24618..25652)
                     /gene="bamC"
                     /locus_tag="ACHUQ7_000581"
     CDS             complement(24618..25652)
                     /gene="bamC"
                     /locus_tag="ACHUQ7_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFR1134135.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            complement(25669..26547)
                     /gene="dapA"
                     /locus_tag="ACHUQ7_000582"
     CDS             complement(25669..26547)
                     /gene="dapA"
                     /locus_tag="ACHUQ7_000582"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFR1134136.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            26693..27265
                     /locus_tag="ACHUQ7_000583"
     CDS             26693..27265
                     /locus_tag="ACHUQ7_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFR1134137.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            27265..27735
                     /gene="bcp"
                     /locus_tag="ACHUQ7_000584"
     CDS             27265..27735
                     /gene="bcp"
                     /locus_tag="ACHUQ7_000584"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFR1134138.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            27988..28605
                     /gene="hyfA"
                     /locus_tag="ACHUQ7_000585"
     CDS             27988..28605
                     /gene="hyfA"
                     /locus_tag="ACHUQ7_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HFR1134139.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGESPLSLTQEQH"
     gene            28605..30622
                     /gene="hyfB"
                     /locus_tag="ACHUQ7_000586"
                     /pseudo
     CDS             28605..30622
                     /gene="hyfB"
                     /locus_tag="ACHUQ7_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            30633..31580
                     /gene="hyfC"
                     /locus_tag="ACHUQ7_000587"
     CDS             30633..31580
                     /gene="hyfC"
                     /locus_tag="ACHUQ7_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HFR1134140.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            31597..33036
                     /gene="hyfD"
                     /locus_tag="ACHUQ7_000588"
     CDS             31597..33036
                     /gene="hyfD"
                     /locus_tag="ACHUQ7_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HFR1134141.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMTLVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            33048..33698
                     /gene="hyfE"
                     /locus_tag="ACHUQ7_000589"
     CDS             33048..33698
                     /gene="hyfE"
                     /locus_tag="ACHUQ7_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HFR1134142.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFATEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            33703..35295
                     /gene="hyfF"
                     /locus_tag="ACHUQ7_000590"
     CDS             33703..35295
                     /gene="hyfF"
                     /locus_tag="ACHUQ7_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HFR1134143.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALALALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMA
                     KPPQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQ
                     RSTWHDFLPSGTASVSEKHSER"
     gene            35285..36999
                     /gene="hyfG"
                     /locus_tag="ACHUQ7_000591"
                     /pseudo
     CDS             35285..36999
                     /gene="hyfG"
                     /locus_tag="ACHUQ7_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            37009..37554
                     /gene="hyfH"
                     /locus_tag="ACHUQ7_000592"
     CDS             37009..37554
                     /gene="hyfH"
                     /locus_tag="ACHUQ7_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HFR1134144.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTH
                     KADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            37551..38309
                     /gene="hyfI"
                     /locus_tag="ACHUQ7_000593"
     CDS             37551..38309
                     /gene="hyfI"
                     /locus_tag="ACHUQ7_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HFR1134145.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            38302..38715
                     /gene="hyfJ"
                     /locus_tag="ACHUQ7_000594"
     CDS             38302..38715
                     /gene="hyfJ"
                     /locus_tag="ACHUQ7_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HFR1134146.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            38745..40757
                     /gene="hyfR"
                     /locus_tag="ACHUQ7_000595"
                     /pseudo
     CDS             38745..40757
                     /gene="hyfR"
                     /locus_tag="ACHUQ7_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
     gene            40779..41651
                     /gene="focB"
                     /locus_tag="ACHUQ7_000596"
                     /pseudo
     CDS             40779..41651
                     /gene="focB"
                     /locus_tag="ACHUQ7_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
     gene            complement(41745..42806)
                     /locus_tag="ACHUQ7_000597"
     CDS             complement(41745..42806)
                     /locus_tag="ACHUQ7_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFR1134147.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPVLMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            43019..44482
                     /gene="bepA"
                     /locus_tag="ACHUQ7_000598"
     CDS             43019..44482
                     /gene="bepA"
                     /locus_tag="ACHUQ7_000598"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFR1134148.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQKRFKPYTKM"
     gene            44503..44862
                     /gene="arsC"
                     /locus_tag="ACHUQ7_000599"
     CDS             44503..44862
                     /gene="arsC"
                     /locus_tag="ACHUQ7_000599"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708333.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFR1134149.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIMVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(44995..45696)
                     /locus_tag="ACHUQ7_000600"
     CDS             complement(44995..45696)
                     /locus_tag="ACHUQ7_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFR1134150.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(45791..47080)
                     /gene="uraA"
                     /locus_tag="ACHUQ7_000601"
     CDS             complement(45791..47080)
                     /gene="uraA"
                     /locus_tag="ACHUQ7_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFR1134151.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(47166..47792)
                     /gene="upp"
                     /locus_tag="ACHUQ7_000602"
     CDS             complement(47166..47792)
                     /gene="upp"
                     /locus_tag="ACHUQ7_000602"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFR1134152.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            48116..49153
                     /gene="purM"
                     /locus_tag="ACHUQ7_000603"
     CDS             48116..49153
                     /gene="purM"
                     /locus_tag="ACHUQ7_000603"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFR1134153.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            49153..49791
                     /gene="purN"
                     /locus_tag="ACHUQ7_000604"
     CDS             49153..49791
                     /gene="purN"
                     /locus_tag="ACHUQ7_000604"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFR1134154.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            49963..52029
                     /gene="ppk1"
                     /locus_tag="ACHUQ7_000605"
     CDS             49963..52029
                     /gene="ppk1"
                     /locus_tag="ACHUQ7_000605"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFR1134155.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            52034..53575
                     /gene="ppx"
                     /locus_tag="ACHUQ7_000606"
     CDS             52034..53575
                     /gene="ppx"
                     /locus_tag="ACHUQ7_000606"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFR1134156.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEILRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(53614..>54722)
                     /gene="pdeF"
                     /locus_tag="ACHUQ7_000607"
     CDS             complement(53614..>54722)
                     /gene="pdeF"
                     /locus_tag="ACHUQ7_000607"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFR1134157.1"
                     /translation="KQKLSHWLSPLLEPGEDVYQLSGNDLALRLNTESHQERITALDS
                     HLKQFRFFWDGMPMQPQIGVSYCYVRSPVNHIYLLLGELNTVAELSIVTNAPENMQRC
                     GAMYLQRELKDKVAMMNRLQQALEHNHFFLMAQPITGMRGDVYHEILLRMKGENDELI
                     GPGSFLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKMPAHRFAINLSPTSVCQARFPV
                     EVSQLLAKYQIEAWQLIFEVTESNALTNVKQAQITLQHLQELGCQIAIDDFGTGYASY
                     ARLKNVNADLLKIDGSFIRNIVSNSLDYQIVASICHLARMKKMRVVAEYVENEEIREA
                     VLSLGIDYMQGYLIGKPQPLIDTLNEIEPIRESA"
BASE COUNT        12760 a        14483 c        14175 g        13304 t
ORIGIN      
        1 tctaaaaaag cagaacatta tgtaacttat tattttctaa gcgaaaattt attgtgcggt
       61 cagactaaca aacttgcgta ccacccgaag aacatgcgaa gatttaagta ttctttcttt
      121 tttggttttt atttttcatt ttctcttttc ttctctttcc ggttctgttc tggttttggt
      181 tttttccttt ttatttttat tctttcttca gggttatgct aaaacaccaa aacatttttc
      241 accacaaaag catccaatca tcttgccaga ccaatcacct taaccggata cactgccgga
      301 tataatccca ctaacaccca gacaccaagc accatacctg ataactaccg tattcaggaa
      361 ggaaatgcga gtgatttcac cgtcaccggc acaaccgatc cgccaaaaag aggcgtacca
      421 atgtcgatat agtcccccgc gcggacaatc acttcgtcaa tgactgccaa cgggagttgt
      481 tgtagctgtg ggcgcaacaa catgcccaga gctttaccaa agtcctgccc ggccaccacc
      541 agcaggggat gcggattcgg aaaacgcgcg acgaaatcga ccagcgcgtt gatgaccgtc
      601 agaaccgcgg cgtaacgcac aggcagcgag gcgggaagcg ccagcacgta cacgtcagtt
      661 ttgggatcaa gatccagctg aagcagcgcc tgttgccagg cactcaccag atccgtttca
      721 tcaatcggga tcgccaccgg caaattgcgc agcggcagtt gtacgccctc cagccagatt
      781 gtgctgccag agagcgaaag ggtatgtgca cccgcgccaa tcaccgtgga gcgtacggtt
      841 tgcgccggaa actgcacatt catctcacgc aggcgcggat ggtcatgcag cgccgttgcc
      901 agaagtgggc caatatcggc aaaacagaac gggttggcgg gctggtggcg ataacattcg
      961 cccacgcctc cagaaagcgt aatgatttcg ggcgtaacac ctgcgggcag caaaccggtt
     1021 tgcatcaatg cctgcgcgag cggcgaaagc gttccgtcaa tcacttcgac aatcagcgcc
     1081 gccatccgcc gggtaacctg caccagctgc gcgccggtca gcgaacgggc gtcagtgcct
     1141 gcaccgaagc actcatccac aatcatctgc cccggtttat gagcgtaaac cacgcgcccc
     1201 tggctgtcgg tttccagcag gcgaccaccg acgttgagac aggcagtgcc gctgattttt
     1261 ccggcatcga acagggcgta gttcgcggtg ccaccgccga tatcgatatt cagtacccga
     1321 cacagccgtt gttcagaaag ggtttgcgcc ccggctccgt gaccggcgat cacggattcg
     1381 aggtgcggcc cggcgctggc gacgacaaaa tcgcccagcg actgtttttc cttcatcact
     1441 ttttccatca gctgtgcgac caggctttcg gtaaactggc cttccggcag ctgggcaatg
     1501 atggtttcgc ggatgcgctg gctttcggtt ttgtcggctg gcacgctggc gggcacggac
     1561 gccgttaccg ggatcgattc atcacattcg gtgatggtaa aacccagtag atccgccact
     1621 tcacgggctt ccggggtaat aatgctggcg cgcagaacca cggacattgc ctgttcgccg
     1681 cgcgcgtgtg cttcacgaat atcattcgct gtgatgagtt ttttcacctg cttcctcctg
     1741 tttggtgaga gatgccagat aatccaccag ttgctgcacg ctttgcgggt cgtggctgtt
     1801 tagctcgaaa atcggctctt cgaaacccgt ctcaagcaat aactttcgcg ttgcggcgac
     1861 gtcagcatct ggcatgtccg ttttactgat gacggcgatt tgccgtttgc tgacgccaat
     1921 atccagcaac ccggcaggca accgactttc cgggtcattt gcgccatgga catagatcag
     1981 catgtctaca tcctgtagcg tggtaattaa ggcgtgatac cagcggggat ggctaaaata
     2041 ttcacccggc gtatcaatat cgcccttatc attaaattcc acggccagtg tttttctggc
     2101 gagggtataa ttcccctgca acgcattaaa taacgttgtt tttcctgccc cgacgctacc
     2161 cacaaacgca attcgtttca tgaatgcctc gtattaactt ttggtcattt cacaaagcgt
     2221 ataatttaat aagcgaccta aaccgctgac ggtttgtgat aacgcttctt ctactgcgcc
     2281 caccgagcca taaatcacca atgcaccgct gaagcggtcg agaaaaccga tatgcacatc
     2341 agcggctttc agcgctaaat caccggcgat catcgccgtt tcgccgggag ttagcgtcat
     2401 aatgccaatt gcgcccgcat ccggaacgcc gattttcttc gccagttcct caccgggatg
     2461 cgcgatgaga tgcgccagtg tgacctgttt gccgggcaca aattcctgaa tgatgcgttc
     2521 tttatccatg agtcgccgcc tgtgaaaaac tttcgtgtat cgtgacaatt tttcgtcaga
     2581 gtaaataaca aaattcggca agcgcgttta aaaggtgagg tagatcaata agaatggaga
     2641 gatatttact gttatgaata aactttcata tgcgacgcat gaatataaat aaaataatat
     2701 cgagatattt tatatactga tttttttatt attcgcaaac tcagtaataa gctttgctaa
     2761 tatatttcag gaaaaccgct taccctgaat attcagggta agcgtgagag ttaagaatga
     2821 attacagcgg ttgggtttgc gcttcaacca cggccagcgc caccatattg acgatacgac
     2881 gcaccgatgc gatcggcgtt aacacgtgaa ccggtttcgc cacgcccatc agcaccggac
     2941 cgacagtcac accttccgag ctggaaacgc gcagtaagtt gtaactaatg cgggcagctt
     3001 ccatgttcgg catcaccaga atattggcag aacctttcaa agggctgtcc ggcatacggt
     3061 cgttgcgaat cgcttccacc agcgctgcat cgccgtgcat ttcaccatca atcatcagat
     3121 ctggcgcacg atccctgacc agttccagcg cctgacgcat tttgctcgac gacgggcagt
     3181 cagaagaacc aaagttggag tgcgacaaca gagcaacgcg cggctcaata ccaaaacgac
     3241 ggacagtttc tgccgccatc aaggtgatct ccgccagctc ttctgcgtct ggttcgtcat
     3301 tgacgtaggt atcggcaata aaggtgttac cactcggcag cagcagcgca ttcattgcac
     3361 ctgcggtgtg aacgccatcg cgataaccaa agacattttt caccacacta aaatgttcat
     3421 gataatcgcc caccgtaccg cagatcattg catcggcttc cccacgctga accatgatcg
     3481 cgccgatcac tgtcgggtta ctgatcagcg cacgctgcgc ctgttcctga gtgacaccgc
     3541 gacgcttcat gatctggaag tattcggtcc agtactcttt aaagcgcgga tcggattcgt
     3601 tattgacgat ttcaaaatca acgcccgctt tgatctgcaa gcccagtttc tgaatgcgca
     3661 tttcgatcac gttcggacga ccgataagga tcggtttcgc cagtcccagc gttaccagtt
     3721 cctgagtcgc atgcagaacg cgcgcctctt ccccttccgg cagaactacg cgcttcggcg
     3781 ctttgcgagc ctgggagaaa atcggcttca taaacaggtt ggttttgtaa acgaactcag
     3841 tcagcttgtc gatgtagacg tcgaaatcag caatcggacg cgtcgccacg cccgactcca
     3901 tcgccgcttt tgcgaccgca ggagcgatct taacgatcaa gcgcggatca aacggttttg
     3961 gaatgatgta ttccggacca aagctcagat cctgaacgcc atacgctgaa gccaccactt
     4021 cactctgttc tgcatgggcc agttctgcaa tcgcgcgtac cgccgccagt ttcatctctt
     4081 cgttgatggc ggttgcgcca acgtccagcg cgccacggaa gatgaacggg aagcaaagaa
     4141 cgttgttcac ctggttcggg tagtcggaac ggccggtaca gatgatggca tccggacgca
     4201 cttctttcgc cagcggcggc agaatttccg gttccgggtt cgccagcgcc aggatcattg
     4261 gtgcccgagc cattttcttc accatttcct gggtcagcac tttcgggccg gaacagccca
     4321 ggaaaatatc cgcaccttca atcacatcat cgagggtacg tttgccgtca tccaccaccg
     4381 catatgcggc tttggtttcc gccatatttg gctcacggcc ctgatagata acgccttttg
     4441 aatcgcaaac cacgatgtta tgtttttgca gccccagcgc taccagcagg ttcatacagg
     4501 cgattgctgc ggcacccgcg ccggaaacca ccatccgcac gtcggagatg tttttctcca
     4561 ccacgcgcaa gccgttgagg atggcggcag tgctgatgat tgccgtgccg tgctgatcgt
     4621 cgtggaatac cggaatattc atccgctcgc gcagtttctg ttcaatatag aaacattctg
     4681 gcgctttaat gtcttcgagg ttgatgccgc cgaaggtcgg ttcgagcgcg gcgacgactt
     4741 caataaattt gtccgggtcg agttcatcaa cttcaatgtc aaatacatca atcccagcga
     4801 atttcttaaa tagaacgccc ttgccttcca tcaccggttt gcctgccagc gcgccaatgt
     4861 tgcctaaccc cagcaccgcc gtaccgttag agatcaccgc aaccaggtta ccgcgcgcgg
     4921 tatatttgta ggcttttagc gagtcttttt cgatttcaag acaaggtgcg gcaacgcctg
     4981 gtgagtaggc cagcgccaga tcgcgctgcg ttgccagagg cttggttgga gaaacctgga
     5041 ttttccctgg aactggaaat tcatggaaat caagtgcact ttgttttaac tggtcatcca
     5101 tttggttgtt cctttcacgt aacgttcaca aataaagtgt gtgggcaaca gcccctgccc
     5161 acaacgtggc gcacattatt accctgccgg agtctacaga ctttgagcaa gtccaaactc
     5221 tcaccattaa tataatgttt tggtaataat cctataacac tgatgttacc tgcttaatcc
     5281 agcaatacca tgcctgtctg ctatgctttt ttgatgcgtt tagcgaaatt tctcagaagt
     5341 gtgaattaac gcactcatct aacactttac ttttcaagga gtatttccta tgaacgagtt
     5401 agacggcatc aaacagttca ccactgtcgt ggcagacagc tgcgatattg agtccattcg
     5461 ccattatcat ccccaggatg ccaccactaa tccttcgctg ttactcaagg ctgccggatt
     5521 atcacaatat gagcatttaa tagacgatgc tatcgcctgg ggtaaaaaaa atggcaagac
     5581 ccaggaacaa caggtggtcg cagcgtgtga caaactggcg gtcaatttcg gtgctgaaat
     5641 tctcaaaatc gtacccggtt gcgtgtcaac agaagttgat gcacgcctct cttttgataa
     5701 agaaaagagt attgagaagg cgcgccatct ggtggacttg tatcagcaac aaggcgttga
     5761 gaaatcacgc attttgatca aactggcttc gacctgggaa ggaattcgcg cggccgaaga
     5821 gctggaaaaa gaaggtatta actgcaacct gacgctgctg ttttctttta cccaggcgcg
     5881 ggcctgtgcg gaagcaggcg tttttctgat ttcgccgttt gtagggcgta tttatgactg
     5941 gtatcaggca cgcaagccga tggacccgta tgtggtggaa gaagatccgg gcgttaaatc
     6001 ggtgcgcaat atctacgact actataagca acaccactat gaaaccattg tgatgggcgc
     6061 gagcttccgt cgcaccgaac aaatcctcgc cttaaccggc tgcgatcgac tgactatcgc
     6121 accgaatttg ctgaaggagc tgcaggaaaa agtttctcca gtggtacgta aattaatccc
     6181 gccttctcag acgttcccac gcccagcacc catgagcgaa gcggagttcc gttgggagca
     6241 caatcaggat gcgatggcgg tagaaaaact gtctgaaggc attcgtctgt tcgctgttga
     6301 tcaacgcaaa ctggaagatc ttcttgccgc caaactataa accagccacg gagtgttata
     6361 tgtcccgaaa agaccttgcc aatgcgattc gcgcactcag tatggatgct gtacaaaaag
     6421 ccaattctgg tcatcccggc gcgccgatgg gcatggctga tattgccgaa gtgctgtgga
     6481 acgattttct taaacataat cctaccgacc caacctggta tgatcgcgac cgctttattc
     6541 tttccaacgg tcacgcgtcg atgctgctct acagtttgct acatctgacc ggttacgacc
     6601 tgccgctgga agaactgaag aacttccgtc agttgcattc gaaaacccca ggccacccgg
     6661 agattggcta tacgccaggc gttgaaacca ccaccggccc gcttggacaa ggtttggcga
     6721 acgccgtcgg gctggcgata gcagagcgta cactggcggc gcagtttaac cagccagacc
     6781 atgagatcgt cgatcacttc acctatgtgt ttatgggcga cggctgcctg atggaaggta
     6841 tttcccacga agtctgttcg ctggcaggca cgctgggact aggcaagctg attggttttt
     6901 acgatcacaa cggtatttcc atcgacggtg aaacagaagg ctggtttacc gacgatacgg
     6961 caaaacgttt tgaagcctat cactggcatg tgatccatga aatcgacggt cacgatccgc
     7021 aggcggtgaa ggaagcgatc cttgaagcgc aaagcgtgaa agataagccg tcgctgatta
     7081 tctgccgtac ggtgattggc tttggttcgc cgaataaagc aggtaaggaa gaggcgcacg
     7141 gcgcaccact gggggaagaa gaagtggcgc tggcacggca aaaactgggc tggcaccatc
     7201 cgccatttga gatccctaaa gagatttatc acgcctggga tgcccgtgaa aaaggcgaaa
     7261 aagcgcagca gagctggaat gagaagtttg ccgcctataa aaaggctcat ccgcaactgg
     7321 cagaagagtt tccccgacgg atgagcggtg gtttaccgaa ggactgggag aaaacgactc
     7381 agaaatatat caatgagtta caggcaaatc cggcgaaaat cgctacccgt aaggcttcgc
     7441 aaaatacgct taacgcttac gggccgatgc tgcctgagtt gctcggcggt tcggcggatc
     7501 tggctcccag caacctgacc atctggaaag gttctgtttc tctgaaggaa gatccggcgg
     7561 gcaactacat tcactacggg gtgcgtgaat ttggcatgac cgctatcgcc aacggcatcg
     7621 cgcaccacgg cggctttgtg ccgtataccg cgacgttcct gatgtttgtt gaatacgccc
     7681 gtaacgccgc gcggatggcg gcactgatga aagcgcggca gattatggtt tatacccacg
     7741 actcaattgg cctgggcgaa gatggtccga cgcaccaggc tgttgagcaa ctggccagcc
     7801 tgcgcttaac gccaaatttc agcacctggc gaccgtgcga tcagatggaa gcggcggtgg
     7861 gctggaagct ggcggttgag cgccacaacg gaccgacggc actgatcctc tcaaggcaga
     7921 atctggccca ggtggaacgt acgccggatc aggttaaaga gattgctcgt ggcggttatg
     7981 tgctgaaaga cagcggcggt aagccagata ttattctgat tgccaccggt tcagagatgg
     8041 aaattaccct gcaagcggca gagaaattag caggagaagg tcgcaatgta cgcgtagttt
     8101 ccctgccctc gaccgatatt ttcgacgccc aggatgagga atatcgggag tcggtgttgc
     8161 cttctaacgt tgcggctcgc gtggcggtgg aagcaggtat tgccgattac tggtacaagt
     8221 atgttggtct gaaaggggca attgtcggga tgacgggtta cggggaatct gctccggcgg
     8281 ataagctgtt cccgttcttt ggctttaccg ccgagaatat tgtggcaaaa gcgcataagg
     8341 tgctgggagt gaaaggtgcc tgatggtgat cgccggatgc tgtatgttcc cctcacccta
     8401 accctctccc cagaagggcg aggggacggt ccgtgcgctt tttagggccg catctgcgat
     8461 tttgtttaac gggtgatcca caacgtgggc ctggcgtctg gcccatgcca gttatcgcag
     8521 gtaggttctg cggcgtaacg caccaggcga aaacgctgac cgtcaaagcg ccagcgcgcc
     8581 tgaatgccac aatcgcttaa tccgcgccct ttcgctaagg tcaccagttc acgcgatttc
     8641 tcatcaaatg ttgcgttcat cagttccagt tcattcgtct cctggccgtt gttgaacggc
     8701 aaacgcaacc gaaccgggcg cgaggctagc ggctttttac gcgacacaat ccatgccaaa
     8761 tcaatggtgt tataggcccc tgcctcacag ctaatcatca tcagtgcttt atcatcggtc
     8821 agcgcagtga cattcacctc acgacgtaac ggatcaagcg agcagcgcag accattcatc
     8881 cgccagtttc cataatccag caaatcgtta cgttcttcga gtgagagtgg cgtcggcgtt
     8941 gggttaacca ccgcgacctc tttcagcgca ggcgcgggcg gtacgctgag aggcggttcg
     9001 tcccctttct tgatccacgc ggtttcactg ccaacgcgct tttgctgagc atcaataaac
     9061 aacaacgccg cttttaagcc gctcagagaa atggtctgat tgccgtcgcg taaggtgatc
     9121 gccttccctt cctgaattat ctgcaaaaac gcggtgatgg ttgccgtatc atcggttact
     9181 aataaccatg gtgaaatccg ccacttgtca ccacttaatg ccaacggctc accatctaat
     9241 agcagccgtg gcgctatctc cccttcagac gcatccggcg acttcaaccc accgcgctca
     9301 atacgtaaaa cggcatcggt atgcgcccca gcgctgcggc tcagggtcat caccagtcca
     9361 ttatgatcgc ccgtattgcg cgccacacaa aaattttgat tattacaggt gacctgccag
     9421 tcggaaaacg cccgttgcgc tggtgccgcc cacaccaaag acgtcggcaa caaagcgaaa
     9481 aaaaagagaa ggaaaatgcg atagcgcatg gacggtacga ccccagaagt tcaaacaaac
     9541 agcacaagtc gtatcttcgt tgcgcaggta gcgcggctca atcggatttg tcggattgag
     9601 ccaataataa acaacgaaat attattgcta tttttcagtc cattaaatgt gacatttgca
     9661 aatagttaag caataacacc gtcttaccgt cacgtatctc gccggttttg atcatctcca
     9721 gcgcctggct gaacggcagc tcgagcactt caatatcttc atcttcaaca ccgccaccgg
     9781 cgttagcgcg ctgattgtca ctgtattcgg cgataaaaaa gtggattagc tcagtcacac
     9841 cgccaggcga catatacagt tcaaataatt tgcgcacttc gcccacttca tagcccgtct
     9901 cttcaatcgc ttctttacga atgcacactt ccggttcgtc gttatccagc agcccggcgc
     9961 aggtttcaat cagctgcccg ctttcattgc cattaaccca ggtagcgaca cggaactgac
    10021 gaatcagaac cacgctcttt ttcttcgcgt tgtacaggag gatcgtcgca ccattaccgc
    10081 gatcgtaaac ttcacgttta tggcggataa cttcgccatc tttgcgcgtg agatcgtaag
    10141 taatgttgtg cagggtgaaa tagttatcgg agagaatttt gtctttaatg agggtgattt
    10201 gttgcgtcat accgactcca cagcgcgaaa tgaacaatta tattacgctg tgaagtcggg
    10261 tttgtctgcg caggctatca gtgagatttg actgatttta cccccagcca gtcaataatc
    10321 ccctgtgccg catgacgtcc ttctgccatt gcggtaacca ccagatccgc accacgcacg
    10381 gcgtcgccac cggcgaagat tttcggattg gtggtctggt aacggtactg gctttccaca
    10441 tccgcgatga tgcggcccca tttgtctacc gttacaccgt gcgactccag ccacggcatc
    10501 ccgtgtggat tgaagccaaa cgccataatc accgcatcgg ctggcatgac aaattcactg
    10561 ccctcaatcg gtacaggtcg ccgacgcccc tgggcatccg gctcgccaag acgcgtgcgc
    10621 aggaaacgaa ttccgcaaac gtgaccttgc tcattcagct caagcgccac cggctggacg
    10681 ttaaattcga agttggcccc ctcttcgcgg gcgttcttca cctctttctt cgagcctggc
    10741 atgttagctt catcacgacg ataagcgcag gtgacgttac tcgcgccgtg gcgcagcgca
    10801 gtacgcacac agtccatcgc ggtgtcgccg ccgcccagta ccacgacgtt aagtccggcg
    10861 gtattgataa acggctcttc cggtagctct tcgagcccca tcacctgttt agtgttggca
    10921 atgaggaacg gcagcgcgtc ataaacgccc ggcgcatctt cattgggtaa acccgctttc
    10981 atggaacggt aagtgcctac gccaacgaag accgcgtcgt attgttccaa aagcaaatcc
    11041 aaagagacat ctttacccac ttcacaattg agttcgaagt gaatccccat cgcgctgaag
    11101 atttcccgac ggcgtgccag cagggattta tccagtttga aagaaggaat gccgaaagtg
    11161 agcaagccac cgatttctgg atggcgatcg tacaccgtca ccgccacgcc attgcgggtc
    11221 agaacatccg cacaggccag ccctgccgga cctgccccga taatcgccac ccgcttgtcc
    11281 actttggtga catgacttaa gtcaggacgc caacctttcg ccaacgcctg atctgaaata
    11341 tagcgttcaa tgttgccgat agttaccgcg ccgtgctcat cgcgaatagt acaggcacct
    11401 tcacacaaac ggtcttgcgg acaaacgcgt ccggtaattt ccggcagggt gttggtctgg
    11461 tgagaaagct cgacggcggc gtcgatgttt ccggctttca ccagttcaat ccactgcgga
    11521 atatggttat gcagcgggca ggtccattca caaacgctgt gctcgccgaa cttaaggcag
    11581 cgcgaggctt cccgttgtgc ctggtcggcg cgaaatggca gataaatttc atcaaaaccg
    11641 gttttgcgcg cttcaatcgc cagtttatcc ggctcgccac gcgcgggcgt tgcctgcatt
    11701 tgttcgactt tactcattac cggcatttct tgtgcagcgg tactggcatg ccacggttga
    11761 tgctcctgac gtgcggtgcg caagcggcgg gatttcgcca tgccggagag tgcgacgtca
    11821 gtgaccagtt gcagcgcgtc cgccgggcaa ttctcgacac aggcaggacc gttttcgcgc
    11881 cctgcacaaa ggtcgcattt atgcgccgtg gcttttactt ttcctgccgc gacgggtgtc
    11941 aggacgattt gcatcgtacc aaacggacag gccaccacgc aggatttaca gccaatacac
    12001 ttttgctgat tgacctgaat gctgtcatca acgtggctga ttgcgccatt agggcagcta
    12061 cgggcgcagg gcgcatcttc acaatggtga caggtcactg cactacgttg ctgctgatgt
    12121 ttgataaccg taattcgggg atgaaaatgg tgttggctca ggacatgttg ctcatcattg
    12181 tgagccatga cacaggcgat ttcacaagca tgacaaccca gacattgctg actgttggcc
    12241 ataataaaac gattcataac gaccttcttt tttggttgta aaaaccttat tctttatatg
    12301 agtgttgtta ttacccgact tacaggggaa ccggcaatgt tcatgtgccc agaataagta
    12361 actatttcgc tataaactgt ggcagatcaa ataatcccat cactgactaa attgcgtttc
    12421 agcgaactgg aacattaatg attttttgtg gagaagacgc gtgtgattgt taaacgaccc
    12481 gtctcggcca gtctggcccg ggcctttttt tacattgtgc tgctgtcgat tctttccacg
    12541 ggtatcgctc tgctaactct ggcgagcagt ttgcgcgacg ctgaggctat caatattgcc
    12601 ggatcgctgc gtatgcagag ttaccgcctg ggctacgact tgcaaagtgg cagtccacaa
    12661 ctcaatgcac atcgccagtt gtttcagcag gcactgcatt caccggtatt aaccaacctc
    12721 aacgtctggt atgtgccaga agcagtaaaa actcgctatg cgcatctgaa tgccaactgg
    12781 ctggagatga ataatcggct cagcaagggc gatttgccgt ggtatcaggc caatattaat
    12841 aattatgtta atcagataga cctgttcgtg ctggctttac agcactacgc tgaacgcaaa
    12901 atgctgctgg tggtggcgat ttccctggct ggcggtatcg gtattttcac gctggtcttt
    12961 tttactttgc gccgcatacg ccatcaggtg gttgccccgc tgaatcagct ggttaccgcc
    13021 agtcagcgta ttgaacacgg gcagttcgac tcgccgccgc tggataccag tctgccgaat
    13081 gagcttggtc tgcttgcaaa aacctttaac caaatgtcga gcgagctgca taaattgtac
    13141 ctttcgctgg aggcgtcagt agaagaaaag acccgcgatc tccacgagac caagcgtcgt
    13201 ctggaggtgt tgtatcagtg cagtgttccc aggcgctgaa caccagccag attgatgtgc
    13261 attgtttccg ccatattttg cagattgttc gcgacaatga agcagctgaa tatctggagc
    13321 tgaacgttgg ggataactgg cggattagcg aagggaagcc aaaccctgag ctgccaatgc
    13381 agattttacc ggtgacaatg caagagacgg tttacggcga actgcactgg caaaatagtc
    13441 acgtttcatc atcagaaccg ctgcttaaca gtgtttcatc gatgctggga cgcggtttgt
    13501 actttaatca ggcgcagaag cattttcagc agttattgtt gatggaagaa cgtgcgacca
    13561 tcgcccgcga attgcacgac tcgctggctc aggtgctttc ttacttacgt atccagttga
    13621 cgttactgaa gcgttcgata ccggaagata acgccaccgc acaaagtatc atggccgatt
    13681 tttcccaggc gttgaatgat gcttatcggc agttacgcga gctgttgact actttccgcc
    13741 tgacgctgca gcaggcggat ctcccctccg cgttgaggga aatgctggat acgttacaaa
    13801 atcaaaccag cgctaaactg accctcgact gccgtctgcc aaccctggca ctggatgcgc
    13861 aaatgcaggt gcatttgttg caaattattc gcgaagcggt gctgaatgcg atgaagcacg
    13921 ccaacgccag cgaaatcgcc gtcagttgcg tcaccgcgcc ggacggcaat cacacggttt
    13981 atatccgtga taacgggatt ggtatcggtg aaccgaaaga acccgaaggt cattatggac
    14041 tgaatatcat gcgcgaacgc gcggaacggc tgggcgggac gctgactttt tcacaacctt
    14101 ccggcggcgg cacgttagtg agtattagct ttcgctccgc agagggtgag gggaagggga
    14161 acacgccggc tgaggtgtaa ataattaccc tcacaccggc cctctccctt taaaggcgag
    14221 agggaaaaac gtgcccatat tgacctgtaa gcgagtgtgt cagcaatctc atgataattt
    14281 acattaactc ctttttttct ccacgattgg ctcgtacctt gccgctacag tgaagcaagt
    14341 caagcctaca acaatacgca gaaacacgag gtcctctttt aatggcgaat ttctttattg
    14401 atcgccccat ttttgcctgg gtgctgacaa tcctgttgtg tctgacaggt accctggcga
    14461 ttttttcatt gcccgttgaa caataccccg atctcgcgcc accgaatgtg cgagtgaccg
    14521 ctaactatcc cggcgcatcg gcccagacgc tggaaaacac cgtgacccag gttatcgagc
    14581 aaaatatgac cggcctcgat aatctgatgt atatgtcatc tcagagcagt ggcaccggtc
    14641 aggcatctgt cactttaagt tttaaagcag gcaccgatcc ggacgaagcc gtgcagcaag
    14701 tacaaaacca gctgcaatca gccatgcgaa agttaccgca ggcggtgcaa aatcagggcg
    14761 tgacggtgcg taaaaccggc gataccaaca ttctgaccat tgccttcgtc tctaccgatg
    14821 gctcgatgga taaacaggat attgctgatt atgttgccag taatattcag gacccgttaa
    14881 gccgcgtgaa tggcgtcggg gatatcgatg cctatggttc gcaatattcc atgcgtatct
    14941 ggctggaccc ggcgaaactc aacagtttcc agatgacggc taaagatgtc actgatgcca
    15001 ttgagtcaca gaacgcgcag attgcggttg ggcaacttgg tggtacacct tccgtcgata
    15061 agcaggcgct caacgccacc attaacgccc agtcactgct gcaaacacca gaacagttcc
    15121 gcgatatcac cttgcgggtg aatcaggacg gctcagaggt aaggctgggc gatgtcgcca
    15181 ccgtcgaaat gggggcggag aaatacgatt atcttagccg cttcaatggt aagccagcct
    15241 ccgggttggg ggtaaaactg gcctccagcg caaacgaaat ggcgacagcg gagctggtgc
    15301 tcaatcgtct cgacgagctg gcgcagtatt tcccgcacgg actggaatac aaggtggcgt
    15361 atgaaaccac ctcgtttgtt aaagcctcca ttgaagacgt ggtgaaaacg ctgctggaag
    15421 ctatcgctct ggttttcctc gttatgtatc tgttcctgca aaacttccgc gccacgctga
    15481 tacccactat cgccgtgccg gtggtgttga tgggaacctt ctccgtactt tacgccttcg
    15541 gttacagcgt caacacctta accatgttcg cgatggtgct ggcgatcggt ctgctggtgg
    15601 atgacgccat cgtggtggtg gaaaacgtcg aacgtattat gagtgaggaa ggactcactc
    15661 ctcgcgaagc cacacgtaaa tcgatggggc agatccaggg ggcactggtc gggattgcga
    15721 tggtgctttc ggcggtattt gtaccaatgg ccttcttcgg cggcaccacc ggtgccatct
    15781 atcgccagtt ctctattacc attgttgcgg cgatggtgct gtcagtactg gtagcgatgc
    15841 tcctcactcc ggctctgtgt gccacgctac ttaagccact gaaaaaaggt gagcatcacg
    15901 ggcaaaaagg tttttttgcc tggtttaacc agatgtttaa ccgcaacgcc gaacgctacg
    15961 aaaaaggggt ggcgaaaatt ctccaccgta gcctgcgctg gattgtgatt tatgtcctgc
    16021 tgcttggcgg catggtgttc ctgttcctgc gtttgccgac gtcgttctta ccgctggaag
    16081 accggggcat gtttactacc tcggtacaat tgcccagcgg ttcaacccaa caacagaccc
    16141 tgaaagtcgt tgagcaaatc gagaaatatt acttcaccca tgaaaaagac aacatcatgt
    16201 cggtgtttgc caccgttggt tctggccctg ggggtaacgg gcaaaacgtg gcgagaatgt
    16261 ttatccgcct gaaagactgg agcgaacgcg acagtaagac cggcacctcg tttgccatta
    16321 tcgagcgtgc aacgaaggcg tttaaccaaa ttaaagaagc tcgcgttatc gccagcagcc
    16381 caccagcaat tagcggtctt ggtagttctg caggttttga tatggagttg caggaccacg
    16441 ctggagcggg tcacgatgcg ctgatggcag cacgtaatca gttgctggcg ctggcggcgg
    16501 aaaacccgga gctaacccgt gtgcgccata acggcctcga tgacagtccg cagttgcaga
    16561 ttgatatcga ccagcgtaaa gctcaggcgc tgggcgttga tatcgacgat attaacgaca
    16621 cactgcaaac cgcctggggt tcgagctatg tgaatgactt tatggatcgc ggtcgcgtga
    16681 agaaagtcta tgtgcaggca gctgcgccgt atcgcatgct gccagatgac atcaatctct
    16741 ggtatgtccg aaataaagat ggcggcatgg tgcccttctc tgctttcgcg acctcacgct
    16801 gggaaacagg ctcgccgcgt ctggaacgct ataacggtta ttctgcggtt gagattgttg
    16861 gggaagccgc accgggggtc agtaccggta cggcgatgga tattatggaa tcgttagtga
    16921 agcagctgcc aaacggcttt ggtctggagt ggacggcgat gtcgtatcag gagcggcttt
    16981 ccggcgcgca ggctccggcg ctgtacgcca tttccttgct ggtggtattc ctgtgtctgg
    17041 cggcattgta tgaaagctgg tcggtaccgt tctcggtaat gctggtcgtg ccgctggggg
    17101 taatcggcgc gctgctggca acctggatgc gcgggctgga aaacgacgtt tacttccagg
    17161 tgggcctgtt aacggtcatt ggtttatcgg cgaaaaacgc catcctgatc gtcgagtttg
    17221 ctaacgagat gaaccaaaaa ggccatgacc tgtttgaagc gacgctccac gcctgccgtc
    17281 agcgtttacg cccgattctg atgacctcgc tggcatttat cttcggcgta ttgccaatgg
    17341 caaccagcac gggtgccggt tccggtggtc agcatgcggt gggtactggc gtaatgggcg
    17401 ggatgatttc agctactatt ctggctattt acttcgtgcc gctgttcttt gtgctggtgc
    17461 gccgccgctt cccgctgaag ccgcgcccgg aataagcaat aaaaaaggcg acatgccaat
    17521 gtgtcgcctt tttcaacttt ccgattaaga acctgctcag cgggttcttg ctgtttatac
    17581 tttgtctcag gaattactta cgaagcataa cttcgataaa gtctttccag ttccccagtt
    17641 cacgttcaat cataacaacc tctcttataa ttatgggtat tctacggaaa caatataccg
    17701 tggtgaagct aatttactcg attgctgcga tgactacctc cgggggacaa accttatgta
    17761 aatactatgg tcctgcggtg ataatttgta tgtgatacac agcaacattt cgagatattc
    17821 atacggcatc taatactgat ttaattctgg ttaaaataca gacagataac aagatgaata
    17881 ttcttaatgt ttacgttaaa aatgtttaat attatttaat agttgttaat ttgaatactt
    17941 cgataatgtt atattttctg ataatcattt gcaggcaaaa tgttttcacc cttaaatgag
    18001 tatttattct cataaatcga aaaaggattc attatggtta cactttacgg catcaaaaat
    18061 tgtgacacca ttaaaaaggc tcgccgttgg ctggaagcca ataacatcga ctatcgtttt
    18121 catgattacc gcgtcgatgg gctggacagc gaattattga acggttttat caacgaatta
    18181 ggctgggaag cgttactcaa cacccgtggt acaacctggc gtaaactgga cgaaactacc
    18241 cgcaataaaa tcaccgatgc ggcctctgcg gcggcattaa tgactgaaat gcctgcaatt
    18301 atcaaacgtc cattgctctg cgcgcctggt aagcctatgc tgctgggttt cagtgattcc
    18361 agttatcagc aatttttcca tgaggtgtag tctatgtcgt gcccggttat tgagctgaca
    18421 caacagctta ttcgccgccc ttccctgagt cctgatgacg caggatgtca ggctttgttg
    18481 attgaacgtt tgcaggcgat cggttttacc gttgaacgca tggattttgc cgatacgcag
    18541 aatttttggg catggcgtgg gcaaggtgaa acgttagcct ttgccgggca taccgacgtg
    18601 gtgccgcctg gcgacgccga tcgttggatc aatccgccgt ttgaacccac cattcgtgac
    18661 ggcatgttat tcgggcgcgg tgcggcagat atgaaaggct cgctggcggc gatggtggta
    18721 gcagcagaac gttttgtcgc acaacatccc aaccatacgg ggcgactggc atttctgatc
    18781 acttctgatg aagaagccag tgcccacaac ggtacggtaa aagtcgtcga ggcgttaatg
    18841 gcacgtaatg agcgtctcga ttactgcctg gtcggcgaac cgtcgagtat cgaagtggta
    18901 ggtgatgtgg tgaaaaatgg tcgtcgcgga tcgttaacct gcaacctcac cattcatggc
    18961 gttcaggggc atgttgccta cccacatctg gctgacaatc cggtacatcg cgcagcacct
    19021 ttccttaatg aattagtggc tattgagtgg gatcagggca atgaattctt cccggcgacc
    19081 agtatgcaga ttgccaatat tcaggcggga acgggcagta acaatgttat tccgggtgaa
    19141 ctgtttgtgc agtttaactt ccgcttcagc accgaactga ctgatgagat gatcaaagcg
    19201 caggtacttg ccctgcttga aaaacatcaa ctgcgctata cggtggattg gtggctttcc
    19261 gggcagccat ttttgaccgc gcgcggtaaa ctggtggatg cggtcgttaa cgcggttgag
    19321 cactataatg aaattaaacc gcagctactg accacaggcg gaacgtccga cgggcgcttt
    19381 attgctcgca tgggggcgca ggtggtggaa ctcgggccgg tcaatgccac tattcataaa
    19441 attaatgaat gtgtgaacgc tgccgacctg cagctacttg cccgtatgta tcaacgtatc
    19501 atggaacagc tcgtcgcgtg atgagtggtt ctgcaagagg aaataagcat ggactggctg
    19561 gctaaatatt ggtggattct ggtgattgtc tttttggtag gcgtcctgct gaacgtgatt
    19621 aaagatctca agcgcgtcga ccataagaaa tttctcgcca acaagccgga gcttcccccg
    19681 catcgtgatt tcaacgataa gtgggacgat gacgacgact ggccgaaaaa ggatcaaccg
    19741 aagaagtaag ctgagtgctc aaccgtaggc cggataaggc gttaacgccg catccggcaa
    19801 tggtgaacga tccctgatgc gacgctcacg cgtcttatca ggccaggtaa tctgtctttt
    19861 acatcatttc aatcacatca tcatcgccag gcttaccgcc acttagcgct tggtcgaaat
    19921 aatgtttcgg aatggtataa cgcaagtgat ccagggcaaa ttgcatactg cgattgtcga
    19981 tcgcatggcc taaatcctcc acgatatcca gcgtcacgtc accaccggca ctgattaacg
    20041 cttcctgcgc cgcaactgca tgcgccagat cgataaccgg atcttcacca ccatgaatca
    20101 agtgaattgt cgtggctgta gacgcggtct ccggcaggct cgcatagcgt ccgttaaagg
    20161 caatgacgcg cgaagcaagg cccggttcgg ctttaatgct ctccagcgcc ataatcgcgc
    20221 cttgtgagaa accaattagc gccgtggcgt tagcccctac tccgctctgt ttctgccagt
    20281 agcgtaccgt ttcaataaac gtcggcatga tggcatccac acgcgcctgg cgattatctt
    20341 ccgtaatacc ctgcactgaa aaccactgac gccccgccgg attaccgctc ggctccgcgc
    20401 caccgacact caccaccagc gcatcaggaa acagcggtgc aaaccagctg cctatttccc
    20461 ccattgccac cgggttatcc ccgacaccgt ggaaaagcag taacaactgt tgtgcaggtt
    20521 tatccgggct ttgaacaaca aaatggtcat gtttcatggc gaactcctta actgatgtca
    20581 ttaattttac gccgctgact cacaatgacc atgggagaaa attgaaggag tcagtgaaaa
    20641 aattgccatt gcgttatgcg atcgcgcaga cgctcagtgc gaacctcatt tagtgcgaac
    20701 agcgcctgcg cggcctcttc ccgctggcga accagtaaca tcttgcggcc tgaaagttta
    20761 agtgtggtac ataactgcgc gtcactggcg tttttctgta aacgcccacg cagcgccggt
    20821 aatgccagtt cactggtttg tagcagacgc aataagcaac ctaacgatgt taatagcgga
    20881 cgatgagcga aagcaaaacc ggccagttca agccagtcgt cgtcagaaag gatggcatcg
    20941 tttagtggat caacaggaag catttcgcca ttccactgcg cgagagcttg cgcatcgcga
    21001 cgtaaacggt aatgctcacg ttcagccagc tgtttacccg catcactcat cggtaacagc
    21061 gccatcgccg tatagcaacc gctgctggct tcccgatgat tacccatccg caccagcaca
    21121 aaaccgcagc gttgccagaa acgccataac tccccagtgt aaccaaaact caccgaaaga
    21181 tagtcgaggt catgcgtata ttgcaaagct ccagcaataa gttgtcgccc tgtgccttcc
    21241 cgctgacgag ccggatgaac tgctatccgg ctaacccgcc ttccgcgcaa tgtcgccgcc
    21301 agtggattgc tgccgtgcgc cgccagcgac tgggccacca gattaccccg cgggcgacga
    21361 aaacctgccc ataccgcctg actgagttct tgagataatc cgccctcatc caccagccac
    21421 aacgccccgg caatctcgtt ttcgccagcc gcctgtaaaa aatgttgccc tggtgcatcc
    21481 atcatccggc gtaaatccag cggcgaggtc cggtagtgcg caccagacaa taactgataa
    21541 acctttaacg gcgtttctgg ctcgcttcgc cataacgttt gttcaaatgc ggaaatgacg
    21601 atattgcctt gtggtgtatg ggtgaagttt tcatcgttaa aaaccagtgc ctcactaacc
    21661 attttttcca gcgggcatcc ctgtgcccag cggatcggct gttgcagttc aaaacggtgt
    21721 aaatgcggaa agcgagcgca aaatttcagc aaaaaaccac gtccggtacc ttcgtagccc
    21781 tgcaccgtag tggttaacaa cgttcgagga aaacgcgata ccagttgatg caacaacggc
    21841 gcaggtatgg ctgcggcttc gtcgaccacc agccagtcgg cttgctcatc gctggctaac
    21901 aaggcatccg gtgcaataaa gcgaaacttc tcgcccgcaa attgtgccag tacatccgtt
    21961 gccgcttttg cgggcgcggt gacaatcgca ctgcccgcaa tacgagaaat gagttgccct
    22021 gccaacgccg acttaccgcg cccacgcgca gccgttaccg ctgccacgcc cggcggcatg
    22081 gtcagtagct gctgtaagag ttgctgttgt tctggttgtg gtgcgccggt cgcggggtgc
    22141 cagtcagtac ggggagtaaa atgcgccaac gagaacggct ggttttgccg ccagaggata
    22201 gcgtcgttat ccgccgtaag tacgcgtttg agatgctgga caaaatgcgg cgtcgcaata
    22261 gggtcagggc aatcactcca gcgcagcgag tcggcatcag gttggttttc ccactcttcc
    22321 catacaggga gtaacaaaac cagccagctt cccgctttca acgttccgct aagtgctgca
    22381 aaggcagcgg catcaaagcc gtggcgggcg tcgaataccg catgccggaa ctcgcgccca
    22441 agtaaagttt gtagcgccga gggtgaacag tggttttcag catctagctg cggcgaaatc
    22501 cacagccagt cgccaggtaa ggcatcacgc aacttaagag catgatcaaa acaccaaccc
    22561 tcttccccgc tcaacaccag caagcggcag atcccttcac gtttcatttg cgctgttaat
    22621 gtgtaaagcg cagtcagttc agccatccct gccccggaaa ttaaatgctt ttaccaaaag
    22681 tattgcattg tgccggatcg ccgctgtcga aaccacgttt aaaccagctg tagcgttgct
    22741 gagaagtgcc atgtgtgaaa ctgtctggta ctactcgccc ctgactttgc tgttgtaaac
    22801 ggtcatcgcc gatggcctgc gccgcattca gcgcctcttc cagatcgcca gtttccagaa
    22861 cgccttgctg ctgcatacta tgcccccaga caccggcaaa acagtcggcc tggagttcca
    22921 tacgcacaga taagcggttt acttccgcct gcgtcgcgtt ttgttgcagt tgacgaactt
    22981 tcggctcgat gcctaacagt ttctgcacat gatgaccgac ttcatgggcg ataacgtacc
    23041 cctgggcaaa atcgccatcc gcgccaagtt tgtctttcat gtcatcatag aaggagagat
    23101 cgatataaac tgtgccatcc gccgggcaat agaacggccc cataatggac tggcccgccc
    23161 cgcagccggt gcgcgtcatt ccacggtaca tgaccagctt cggttgctga taggtcttac
    23221 ccatcttttc gaactgttgt ccccaggtgt cttccgtggt tgcctgaatc accgaggtga
    23281 attttgcggc ttcgtcttca ttagggctaa ttgaccgcgt tgattgttgt tgagaaaccg
    23341 gctgcccggt catcaacccg gttaaatcaa cgccatagta gcctgcaacc agcacgacta
    23401 tcagtaaaat cagcccgcct ttaccgctcg gcagacggaa accgggaccg cccattgacg
    23461 gaccgccaga gctgttgcgc ctgtcttcaa cattgtcact ttcacgtcgc ccttgccaac
    23521 gcatagatac ctcaacaata tattcattat agaaatgatc gtaggtggtt cagaggaaga
    23581 ttaccacagg aaagcgggat gaaatggcgg aggaatgagc gttcaatgca tatattccaa
    23641 gagcggttgt cttgccgtgc cagctgcacg gcaagatgat gaacagaaaa atcagtccag
    23701 ctgtacaccc aggcggcggg cgacggcttc ataggcttcg atcaggccac cgaggctctg
    23761 gcggaaacgg tctttgtcca ttttctccag tgtttctttg tcccacaggc ggctgccgtc
    23821 cggggagaac tcatcgccca gtaccacttc acctttgtac agaccaaact ccagcttgaa
    23881 gtcgaccaga atcagaccag catcatcgaa cagttttttc agcacgtcgt tcgctttgta
    23941 ggtcagctct ttcatacgcg ccaggttctc tttgctcacc cagccaaagg tttcgcagta
    24001 agattcgttg accatcggat cgtgcatggc atcgtttttc aggaacagat cgaacagcgg
    24061 cgggttcagc tcaatacctt cttcgattcc aagacgtttc actagagagc cagcagcacg
    24121 gttacgcacg acacactcaa ccggcaccat atccagcttt ttcaccagac attcggtatc
    24181 ggagagcaga cgctccattt gagtcgggat acccgcttca gccagtttgc tcataatgaa
    24241 gtagttgaac ttgttgttca ccataccttt gcgatcaaac tgctcaatgc gcgcgccatc
    24301 ccctgctgac gtatcattgc ggaattcgag caccaacagg tccgggtttt ccgtgctgta
    24361 tacggttttc gctttaccac gatacaactc agcttgcttt tgcatcttta tcactcctgg
    24421 gtgtgaatta acgttttaaa atcttttgct gtctggtgtg ccggatgttt tgtcggatgc
    24481 ggcgtgaacg ccttatccga actacgtccg acaaacaaat ttcgtgcgaa ttaccgctaa
    24541 aatcgcgttt tcctgccgac gcacacgttt gcgtatcata tcagaaaaaa gggccggatg
    24601 attccagccc tgtattttta cttgctaaac gcagcctgga agacagctac cagcgcgtcg
    24661 ttctgactct gagtcagagt atgacctttc ggatcgatga actgtaggct gctgcggtta
    24721 tctaaatcgc caacctgcag tttatagtca ccggatgcca ggcctggatc gctcgcgccc
    24781 agttcctgcc agtcgctgtc agacagcggc ttataagtta cggccatgtt gccctgcgaa
    24841 cgggtgctgt cggtcacttt catgcccact ttttccagcg ccgctggcag acgttgccaa
    24901 accacattga acggcccgcg tacgaccagc attggtaaac cggtgtcatc agctgcactt
    24961 tgtacgtcca tagtggtgga ggcacgattt tgcgcagcgt tcgcggcgtc agtggcagat
    25021 ttatccagac cggcggaaat aacgttcatc atctccgtgc tgtaacgctg catggaagcc
    25081 gcgtctgcaa ccggtttgcc cgcctgttcc aggttcagca gtttaaccgt aaccgcctgc
    25141 tgataaccct gcggcttaac agagatttga taacgaccac gatactgctc gtcttcgtcc
    25201 agacggttcc attgtaccca atcggtggtc agtgtctgac cagcatcatc acgttgggtg
    25261 atggtgtagt ttttcgcctg cagcacgcta accacctgcg gccacagagt attgccacga
    25321 ccattttcca ccagcaatga agcggtatcg cccgtgaact gggtacgcgc gccagaaacc
    25381 agtgccaacg gctgggctgg tggacgaatg tccagcgcct taccgacagc accactaccg
    25441 ttggtcaccg ggattgcata atcaccggag gtcaccggca aaatcattcc agccggggca
    25501 tgaagctccg caagcggtgc cgcttccagg taggcttcat caccactgac ctgacgctta
    25561 tagcgtgagt cagaactaca ggcagcgagt aataaaacaa gcgaaacacc cgcaaccttt
    25621 gccaggcgcg acttttgaac agagtaagcc atcaaatctc cctaaacttt acagcaaacc
    25681 ggcatgctta agcgccgctc tgaccgtctc acgaccactg tcggtgattg gtgtcattgg
    25741 caggcgcagc gtatcggtcg ccacaagacc cagttcctta catgcccatt tcaccgggat
    25801 tggattgggt tcgacaaata gtttgttgtg taatggcatc agacgctgat taataacgcg
    25861 tgcctcggca aaatgccctt ctgctgccag tttgcacatc tgggccatat cacgcgctgc
    25921 gacgttagcc gtaacggaaa taactccatg accgcccaat tgcatgaagt ccagcgcgct
    25981 cgcatcatcg ccgctcagca gaacaaaatc atctgaaacc agctctttga tctggtttac
    26041 acgcgttaag ttccctgttg cctctttgat tccgataata ttttttactt tcgccagacg
    26101 gcccaccgtt tccgggagca gatcgcagcc agtacgggac ggcacattat acagaatttg
    26161 cggcaggtca gtatgctcag cgatggcttt gaaatgctga tacaaacctt cttgcgacgg
    26221 acgattgtag taaggggtta ccgtcaggca gccgacgata ccactgtcat tgaagcgctg
    26281 cgtcaggcta atggcttccg cagtagcgtt agcgccggtc ccggcaatta ccggaatgcg
    26341 cccatcagcc agatccagcg tcatcatcac cacatcagca tgttcgtcat gatttaaggt
    26401 agcggactcg ccagtggtgc caacagaaac gatcgccgaa gtaccgctgg cgacatgata
    26461 atcaatcagt tttttcaagc tagcccgaca gacattacct ttttcatcca tcggagtaac
    26521 aatcgcgaca atacttcccg tgaacatggg ccatcctctg tgcaaacaag tgtctcaatg
    26581 gtacgtttgg tatggcatta aaagcaagca gacagaaccg ttctgattgt tgtatgcatg
    26641 atttttttat gctttcctta agaacaactc accccttaaa ggaataacca gtttgacact
    26701 gtcatcgcaa cattatctgg tgatcactgc gttgggtgcc gatcgccctg gaattgtgaa
    26761 caccatcacc cgtcatgtca gtagttgcgg ctgtaatatt gaagacagtc gcctggcgat
    26821 gctgggagaa gagttcacgt ttatcatgct gctttccggt tcatggaatg ccatcactct
    26881 gattgaatca acgttaccgt tgaaaggtgc cgaactggat cttttaatcg tgatgaagcg
    26941 cacgacggcg cgtccgcgtc cgccaatgcc agcatctgtc tgggttcagg tcgatgttgc
    27001 agactccccg catttaattg aacgcttcac agcacttttc gacgcgcatc atatgaacat
    27061 tgcggagctg gtgtcgcgca cacaacctgc tgaaaatgaa cgggctgcgc agttgcatat
    27121 tcagataacc gcccacagcc ccgcatctgc ggacgcagca aatattgagc aagcgttcaa
    27181 agccctatgt acagaactca atgcacaagg cagtattaac gtcgtcaatt attcccaaca
    27241 tgatgaacag gatggagtta agtaatgaat ccactgaaag ccggtgatat cgcaccgaaa
    27301 tttagcttgc cggatcaaga cggagaacaa gttaatttga ccgacttcca gggacagcgt
    27361 gttctggttt atttctaccc gaaagccatg acccccggct gtaccgtaca ggcctgcggc
    27421 ttacgcgata acatggatga gttgaaaaaa gcgggcgttg atgtgctggg tatcagcacc
    27481 gataaacccg aaaaactctc ccgttttgcg gaaaaagagc tgcttaactt tacgctcctg
    27541 tctgatgagg accaccaggt gtgcgaacaa ttcggcgtct ggggtgaaaa gtccttcatg
    27601 ggcaaaacct acgatggtat tcatcgcatc agcttcctga ttgacgctga tggcaaaatc
    27661 gaacatgtct ttgacgattt caaaaccagc aatcaccacg acgttgtgct gaactggctg
    27721 aaagaacacg cctgattact ttgctccatt ccgtgctggc tgcgcttgcg gccagcatac
    27781 ctcacttctc gtgatcaaga tcacattctc gctttcccct gcgacacggg tgtcgaatcc
    27841 attttttgct gaacgttaat gaccatcatt tttgtaccgt tcagaatcca gttaatacat
    27901 aacttattga atagattgag ttaatcagaa tggcatcctt tatacaatat gaaatgcaat
    27961 gtttcatatc attttcaagg agccgacatg aaccgctttg tggtggccga accactgtgg
    28021 tgtacaggat gtaatacctg tctcgctgcc tgttcggacg tgcataaaac gcaaggttta
    28081 cagcaacacc cgcgcctggc cctggcgaag acgtcaacaa tcactgcccc tgtcgtgtgt
    28141 catcactgtg aggaagcccc ttgcctgcag gtctgcccgg tcaatgccat ctctcagagg
    28201 gatgatgcga tccaactcaa cgaaagcctc tgtattggct gcaagctttg cgccgtggtc
    28261 tgcccatttg gcgcaatcag cgcttcagga agccgtccgg tgaatgccca tgcgcaatat
    28321 gtttttcagg ctgaaggctc actcaaagac ggcgaagaaa acgtgccgcc ccaacatgcg
    28381 ttgctgcgct gggaaccggg tgtccagacc gtcgcggtga aatgcgacct gtgtgatttc
    28441 ttgccagaag gtccggcctg cgttcgcgct tgcccgaatc aggcgttacg gctgatcacc
    28501 gacgatagcc tgcaacgtca gatgaaagaa aaacagcgcc ttgccgcaag ctggtttgcc
    28561 aatggcgggg agtctcccct ttccctcact caggagcaac actaatggat gccctgcaat
    28621 tattaacctg gtcgctgatc ctctatctgt ttgccagtct ggcttcgctg tttttactcg
    28681 gtctggacag actggctatt aagctttccg gcatcacatc gctggtgggc ggcgtgattg
    28741 gcatcatcag cggaattacg caattacatg ctggtgtaac tttagtcgcc cattttgcca
    28801 ccccttttga ctttgccgat ttaaccctgc gaatggatag cctctcggca tttatggtgc
    28861 tagttatctc cttgctggtg gtggtttgtt cgctctattc attgacttat atgcgcgaat
    28921 acgagggcaa aggcgcggcg gcgatgggct tctttatgaa tcttttcatc gcatcgatgg
    28981 ttgccctgct ggtgatggac aacgcttttt ggttcatcgt gctgtttgaa atgatgtcgc
    29041 tgtcttcctg gtttctggtc attgccaggc aggataaaac gtcgatcaac gctggcatgc
    29101 tctacttttt tatcgcccac gccggatcgg tgctgattat gatcgccttc ttgctgatgg
    29161 ggcgcgaaag cggcagcctc gattttgcca gtttcgcacg ctttcacttt ctccggggct
    29221 ggcgtcggcg gtgtttctgc tggccttttt cggttttggc gcgaaagccg ggatgatgcc
    29281 gttgcacagc tggttgccgc gcgctcaccc tgccgcacca tcgcacgctt cggcgttgat
    29341 gtctggcgta atggtcaaaa tcggtatttt cggcatcctg aaagtagcga tggatctgct
    29401 ggcgcaaacg ggtttgcctc tgtggtgggg cattctggtg atggcgatcg gcgcaatctc
    29461 cgcgctcctg ggcgtgctat atgcgctggc ggaacaggat atcaaacggc tgctggcctg
    29521 gagtaccgtc gaaaacgtcg gcattatttt gctggcagtc ggtgtggcga tggacggtct
    29581 gtcactgcac gacccgctgc tcaccgtggt tggactgctc ggcgcactgt ttcatctgct
    29641 caaccatgcg ctgttcaaag ggctgctatt tctcggcgcg gacgcgatta tttcgcgttt
    29701 gcatacccac gacatggaaa aaatgggggc actggcgaaa cggatgccgt ggacagccgc
    29761 agcatgcctg attggttgcc tcgcgatatc agccattcct ccgctgaatg gttttatcag
    29821 cgaatggtac acctggcagt cgctgttctc actaagtcgt gtggaagccg tagcgctaca
    29881 acttgcgggt cctattgcta tggtaatgct ggcagtcact ggtgggctgg cagtaatgtg
    29941 cttcgtcaaa atgtacggta ttactttctg tggtgcgccg cgcagtacac acgctgaaga
    30001 ggcacaggaa gtgccaaata cgatgatcgt cgccatgcta ctgctcgcgg cactctgcgt
    30061 attaattgcg cttagtgcca gttggctggc accgaagata atgcatattg cccatgcgtt
    30121 taccaatacc cctcccgtca ctgtcgccag cggaatagca cttgtacccg gcacgtttca
    30181 tacacgggtc actccttcat tactgttgct gttactactg gcgatgcctt tgctgccagg
    30241 cctttactgg ctgtggtgtc gttcgcgccg cgcagcgttt cgtcgcacag gagatgcctg
    30301 ggcatgcggc tacggctggg aaaacgcgat ggctccgtca ggcaatggcg tgatgcagcc
    30361 gctgcgtgtg gtcttctctg cgttatttcg tctacgacaa cagctcgacc ctacgctgag
    30421 gctaaataaa ggtcttgcgc acgtcaccgc cagggctcag agcacagaac ccttctggga
    30481 tgagcgggtg atccgcccca tcgtgagcgc cacccaacgg ctggtcaaag aaatacagca
    30541 tctgcaaagc ggcgactttc gtctctattg cctgtatgtg gtcgccgcac tggttgtgct
    30601 gctaatcgct attgccgtct aaggaaatca ccatgagaca aactctttgc gacggatatc
    30661 tggtcatttt tgcgttagca caggccgtga ttctgctgat gctaacccca ctttttacgg
    30721 gtatttcccg gcagatacgc gcgcgtatgc actcccgccg cgggccgggg atctggcagg
    30781 attatcgcga tatccacaaa ctgtttaaac gccaggaagt tgcgccgaca tcttcaggtc
    30841 tgatgttccg cctgatgccg tgggtattaa tcagcagcat gctggtgctg gcgatggcct
    30901 taccactatt tattaccgtt tccccttttg cgggcggcgg cgatctgatc acccttatct
    30961 atcttcttgc cctgtttcgt tttttctttg ctctttccgg gctggatacc ggaagtccgt
    31021 ttgcgggagt cggtgccagt cgcgagttga cgctcggcat tctggtcgaa ccaatgctta
    31081 ttctctcact gctggtattg gcgctgatag caggttccac gcatatcgag atgatcagca
    31141 atacgctggc tatgggctgg aactcgccgc taaccaccgt actggcgtta ctggcctgtg
    31201 gttttgcctg cttcattgag atgggaaaaa ttccctttga tgttgctgaa gcagaacagg
    31261 aattacagga aggcccgctg accgaatatt ccggtgccgg gctggcgcta gcgaaatggg
    31321 ggctggggct gaaacaggtc gtgatggcag cactgtttgt ggccctgttt ctgccctttg
    31381 ggcgcgcgca agaactttct ctcgcctgcc tgctgacttc acttgtcgtt acgctgctca
    31441 aggttttgct gatttttgta ctggcctcaa tcgcagaaaa cacgctggca cgcgggcgtt
    31501 ttttactcat tcaccatgtg acctggcttg gcttcagcct tgctgcgctt gcatgggtct
    31561 tttggttaac cggtctgtaa ggagcactga cggaatatgg aaaatcttgc tctgacgacg
    31621 ttaatgctgc cttttatcgg cgcactggtc gtttcgtttt cgccacaacg tcgggccgcc
    31681 gaatgggggg ttttgttcgc cgcgctgacc acgctgtgca tgttgtcgct gatctccgcg
    31741 ttttatcagg ccgataaagt tgccgtcacg ttgacgttgg tcaacgtggg ggatgtggca
    31801 ttatttggcc tggtcattga tcgcgtgagt acgctgattc tgtttgtggt ggtgtttctc
    31861 ggtttgctgg tcacgatcta ctccacgggt tatctgacgg ataaaaatcg cgaacacccg
    31921 cataacggca cgaatcgtta ttacgcattt ttgctggtgt ttatcggcgc gatggcggga
    31981 ctggtactct cctcgacgct gctcggtcag ttgttgtttt ttgaaattac gggcggctgc
    32041 tcctgggcgt tgatcagtta ttaccagagc gataaagcgc agcgttcagc actaaaagcg
    32101 ttgcttatca cccatatcgg ttcgttgggg ttgtatcttg ccgccgccac gctgtttttg
    32161 cagaccggaa cgtttgtgct tagcgcgatg agcgagttac atggcgacgc acgttatctg
    32221 gtttatggcg gcatcctgtt tgccgcgtgg gggaaatcgg cccagctacc gatgcaagcg
    32281 tggctaccgg acgcaatgga agcgccaaca ccgatcagcg cctatctcca cgccgcatcg
    32341 atggtgaaag tgggcgttta catttttgcc cgcgccatta tcgacggcgg caatatcccg
    32401 catgtgattg gcggcgttgg catggtcatg acactggtca ccattcttta tggctttctg
    32461 atgtatttgc cacagcagga tatgaagcgg ttgctggcct ggtcgaccat cactcaactt
    32521 ggctggatgt tcttcggctt gtcgctctcc atcttcggct cgcggctggc gctggagggt
    32581 agcatcgcct acatcgtcaa ccacgcgttc gctaaaagcc tgtttttcct tgtagcaggt
    32641 gcgctgagtt acagctgcgg cacgcgcttg ttgccgcgtc tgcgtggcgt attgcacacc
    32701 ctgccgttgc caggcgtggg tttctgcgtg gcagcgctgg caattaccgg tgtgccgccg
    32761 ttcaacggct tcttcagtaa attcccgctg tttgccgccg gttttgcgtt gtcagtggag
    32821 tactggatcc tgctgcccgc catgattctg ctgatgattg aatcggtcgc cagtttcgcc
    32881 tggtttattc gctggtttgg tcgcgtcgtg ccgggcaaac cgagcgaggc cgtcgccgat
    32941 gccgcaccgc tgccagggtc aatgcgcctg gtgttgattg tactgattgt gatgtcgctg
    33001 atttccagcg taatcgccgc gacctggttg cagtaaggag atgatgaatg accggttcta
    33061 tgatcgtaaa taatctggcg ggactgatga tgctgacatc gctgtttgtg attagcgtca
    33121 aaagctatcg cctgtcatgc ggattttacg cctgccagtc gctggtgctg gtgtctattt
    33181 tcgccactct ctcgtgcctg ttcgccacag agcaactgct gatctggtcc gccagcgcct
    33241 ttatcaccaa agtgctgctg gtaccgttaa tcatgactta cgctgcacga aatattcccc
    33301 agaacatccc ggaaaaagcg ttattcggtc cggcaatgat ggcactgctc gcggcgttaa
    33361 ttgtcctgct ttgcgcattt gtcgttcagc ccgtgaagct accgatggct accgggctga
    33421 aaccggcgct ggcggtagcg ttaggtcatt ttctgcttgg cctgctgtgc attgtcagcc
    33481 aacgcaatat cctgcggcaa atttttggtt actgcctgat ggaaaacggc tcccatctgg
    33541 tgctggcgct tcttgcctgg cgagcaccgg aactggtgga aataggtatc gctaccgacg
    33601 ccatcttcgc cgtcattgtg atggtgttac tggcaagaaa aatatggcgt acccacggca
    33661 cgctggacgt gaacaacttg accgcgctga agggataatg agatgagtta ttctgtgatg
    33721 ttcgctttac tcctgctcac gccgctgctt ttttcgctgc tctgttttgc ctgccggaaa
    33781 cgggggctct ctgcgacttg tacggtgact gtattacata gcttagggat cacactgctg
    33841 ctgattctag cactctgggt ggtccaaact gccgctgatg caggagaaat attcgctgcg
    33901 ggactgtggc ttcatattga tggtctgggc ggtttgttcc tcgccattct tggtgtgatt
    33961 ggctttctca ccggtgttta ctcgattggc tacatgcgtc atgaagtggc acacggcgag
    34021 ctttcacccg ttacactgtg cgattactac ggtttcttcc atctgttttt gttcaccatg
    34081 ctgctggttg ttaccagcaa taacctgatt gtgatgtggg cggcgatcga agccaccacc
    34141 ttaagctcgg cgtttctggt aggcatttac ggtcagcgtt catcgctgga atcagcatgg
    34201 aagtacatca ttatttgtac tgttggtgtc gcttttggtc tgttcggtac cgtgctggta
    34261 tacgccaacg ccgccagcgt tatgccgcag gcagaaatgg cgatattctg gagcgaggtt
    34321 cttaagcaat cgtccttgct tgacccaaca ttaatgctgt tggcctttgt gtttgtgcta
    34381 attggctttg gtaccaaaac cgggctattt cccatgcacg cctggctgcc ggatgctcac
    34441 agtgaagcgc cgagtccggt cagcgccctg ctctccgccg tattgctgaa ctgcgcgctg
    34501 ttggtgctga ttcgctatta catcattatt tgccaggcca tcggcagcga tttccccaac
    34561 cggttgttgc tcatcttcgg catgttgtcg gttgccgtgg cggcattttt cattctggta
    34621 cagcgggaca ttaagcgtct gctggcgtac tccagcgtgg agaacatggg gctggtcgcg
    34681 gtggcgctag gcattggcgg gccgctggga atttttgccg cgctgctgca caccttaaac
    34741 cacagtctgg caaaaacgct gctgttctgc ggttccggca atgtactgct caagtacggc
    34801 acgcgcgatc tcaacgtcgt ctgtgggatg ctcaaaatca tgccatttac cgccgtgctg
    34861 tttggcggcg gtgcgctggc gctggcgctg gcgctggcag ggatgccgcc cttcaacatt
    34921 tttcttagcg aatttatgac cgttaccgca ggactggcac gtaatcacct gctgattatc
    34981 gtcctgctgt tattgctgtt aacgctggtg ctggcgggcc tggtacggat ggctgcgcgg
    35041 gtgttaatgg cgaaaccgcc gcaggccgtt aaccggggtg atctcggctg gttgaccacc
    35101 tcgccaatgg tgattctgct ggtcatgatg ctggcgatgg gaacgcatat tccacaacct
    35161 gtcatcagga tcctggcggg cgcttccact atagtcctct cagggacgca cgacctgcct
    35221 gcacaacgta gcacctggca tgattttttg ccttcaggca ccgcatctgt ttcggagaaa
    35281 cacagtgaac gttaattcat cgtcaaatcg tggcgaagcg attctcgccg ccctgaaaac
    35341 gcagttcccc ggcgcggtgc tggatgaaga gcgacaaacg cctgaacagg tcaccattac
    35401 ggtgaaaatc aatctgctgc ctgacgttgt acattatctt tattatcaac atgatggctg
    35461 gcttccagtc ctgtttggca acgacgagcg gacacttaac ggtcattacg cggtttatta
    35521 tgccctttca atggaagggg ccgaaaaatg ctggatcgtg gtgaaggcac tggtcgatgc
    35581 cgacagtcgg gagtttccgt cagtcacacc gcgcgtccct gccgcggtct ggggcgagcg
    35641 agaaattcgc gatatgtacg ggctgattcc ggttggcctg ccggatcagc gtcgcctggt
    35701 gttgcccgat gactggccgg aagatatgca tccgctgcgc aaagatgcga tggattatcg
    35761 actgcgccct gaaccgacga cagattccga aacgtatgcg tttatcaatg agggcaacag
    35821 cgatgcgcgg gtgatccctg tcggcccgct gcatatcacc tcctatgaac cgggtcactt
    35881 ccgcttgttt gtggatggcg agcaaattgt cgatgctgat taccgcctgt tttatgtcca
    35941 tcgcggcatg gagaaactgg cagaaacgcg gatgggctac aacgaagtga ccttcttatc
    36001 ggaccgcgtg tgtgggattt gcggttttgc ccacagtgtg gcctatacca actcggttga
    36061 aaatgcactg gggattgagg tgccgcaacg agcacatact attcgctcga ttctgctgga
    36121 agtcgagcgg ctacacagtc atttgctcaa ccttggcctc tcctgccatt ttgttggttt
    36181 tgataccggc tttatgcaat ttttccgcgt gcgggaaaag tcgatgacga tggcggaatt
    36241 gctgaccggg tcgcgtaaaa cctacggtct gaatctgatt ggtggtgttc gccgcgatat
    36301 tctcaaagag caacgtctgc aaacgctgaa actggtgcgc gagatgcgcg ccgacgtgtc
    36361 ggagctggta gagatgctgc ttgctacgcc gaatatggaa caacgcactc agggcattgg
    36421 cattctcgac cgacaaatcg cccgtgatta tagccctgta gggccgctga tccgcgcagt
    36481 ggttttgccc gtgatttgcg ctttgatcac ccctacgccg actacggcaa tattccaaaa
    36541 acactgttta cctttaccgg cggcgatgtt ttctcccgcg tgatggtccg tgtcaaagag
    36601 acgtttgatt cgctggcaat gctggaattt gccctcgaca acatgccgga taccccactg
    36661 ctgaccgaag gctttagcta taaacctcac gcattcgcgc tgggctttgt tgaagcgcca
    36721 cgcggtgaag acgtgcactg gagcatgctc ggtgataacc aaaaattgtt ccgctggcgc
    36781 tgtcgtgccg ccacctacgc caactggccg gtgttgcgtt acatgctgcg cggcaatacc
    36841 gtttctgacg caccgctgat tatcggtagc cttgatccct gctactcctg taccgaccgt
    36901 gtgacgctgg tagatgtgcg caagcgccag tcaaaaaccg tgctgtataa agagatcgaa
    36961 cgctacggca ttgatcgtaa ccgttcgccg ctgaagtaag gacagaagat gctgaagtta
    37021 ctgaaaacta ttatgcgcgc cggaaccgcg acggtgaaat atcccttcgc gccactggag
    37081 gtcagcccag gctttcgcgg gaaaccggac ctgatgccca gccaatgtat tgcctgcggt
    37141 gcctgcgcct gtgcttgtcc ggcaaatgcg ctgactatcc agaccgacga ccagcaaaat
    37201 tcgcgcacct ggcagctcta tctggggcgt tgtatttact gcggacgttg tgaagaagtg
    37261 tgcccgacca gagccatcca gcttaccaat aactttgaac tgaccgtcac ccataaagcc
    37321 gatctctata cccacgcgac gttccatcta caacgttgca gccgttgcga acgcccgttt
    37381 gccccgcaaa aaaccgtcgc actggctgct gaattgttgg cacagcaaca aaatgctcca
    37441 caaaaccgcg aaatgttatg ggcgcaagcg agcgtctgcc cggagtgcaa acaacgcgca
    37501 acgctgatca acgacgatac agatgtaccg ctggtggcta aggagcagct atgagtccag
    37561 tgcttacgca atatgtcagc cagcccatca cgctggacga gcaaacgcaa aagatgaagc
    37621 ggcatttgct acaggatatc cgtcgctcgg cttacgttta tcgcatcgat tgcggcggct
    37681 gcaacgcctg tgaaatcgaa atttttgctg ccatcacccc cgtattcgac gcagaacgtt
    37741 ttggcattaa ggttgtttca tcaccgcgtc acgccgatat tttgttattt actggcgcag
    37801 tcacccgggc gatgcgtatg cctgcacttc gggcgtatga gtctgccccc gatcataaaa
    37861 tctgtgtttc ctacggcgcg tgcggtgtcg gcggcggtat tttccacgat ctctacagcg
    37921 tctggggcgg cagcgacacc attgtcccca ttgatgtttg gatccccggc tgcccgccaa
    37981 caccggccgc caccattcac ggtttcgccg tggcgctcgg tttgctgcaa cagaagattc
    38041 acgcggtgga ttatcgcgat cccaccgggg tgactatgca accgttgtgg ccgcagatcc
    38101 cgccatcaca gcgtatcgcc attgagcgag aagcgcggcg gctggcgggc tatcgtcagg
    38161 ggcgagaaat ttgcgatcgg ctcctgcgcc atttaagcga cgatcctaca ggaaatcggg
    38221 ttaacacctg gttgcgcgat gccgacgatc cacgtctcaa tagtatcgtt cagcaactct
    38281 ttcgcgtact ccgggggtta catgactgaa gagtgcgggg aaattgtttt ctggacgctg
    38341 cgaaaaaagt ttgtcgccag tagcgacgag atgccggaac acagctctca ggtaatgtat
    38401 tactcgctgg ctatcggtca tcacgttggc gtgattgatt gtctgaatgt cgccttccgc
    38461 tgcccactga cggaatacga agattggctt gcactggtcg aagaggagca agcccgacgt
    38521 aagatgctgg gggtgatgac ttttggtgag attgttattg acgccagcca caccgccctg
    38581 ttgacccggg cattcgcgcc actggcagat gacgcgacgt ctgtgtggca agcgcgtagc
    38641 attcaattca ttcatctgtt ggatgaaatt gtgctggaac cggctatcta tctgatggcc
    38701 agaaaaattg cgtgagaagg atttctcatt aataaggact gttgatggct atgtcagacg
    38761 aagcgatgtt tgccccgccg caaggaataa caattgaagc ggtaaacgga atgctcgcgg
    38821 agcggttagc gcagaaacac ggcaaggcgt ctttattacg cgccttcatc ccgctgccgc
    38881 cgccgttcag cccggtacaa cttattgaac tgcatgttct caaaagcaac ttctattacc
    38941 gctaccatga tgatggcagc gatgtgacgg caacaacaga gtatcagggc gagatggtcg
    39001 attattcgcg ttacgccgtc cttctcggca gtagtggaat ggcggagcta cgctttattc
    39061 gtacccacgg cagccgtttt actccccagg attgcacact gtttaactgg ctggcacgca
    39121 ttatcacccc ggttctgcaa tcatggctca atgatgaagc acagcaggtg gcgctgcgtt
    39181 tgctggagaa agatcgcgat catcatcggg tactggtgga tatcactaat gcagtgctgt
    39241 cacatcttga tctcgacgat ctgatcgctg acgtcgctcg tgagatccat cattttttcg
    39301 gtctggcttc agtcagtatg gtactgggcg atcatcgaaa gaacgagaag ttcagcctgt
    39361 ggtgcagcga tctttctgcc tcacattgtg cgtgtctgcc acacaatatg cctggcgaca
    39421 gtgtattgct gacacaaacg ctacaaaccc gacaaccgac cttgacgcac cgtgcagatg
    39481 atctgtttct ctggcaacgc gacccgttat tactcttact tgcatctaac ggctgcgaat
    39541 ctgcgctcct tataccgctt acctttggca accatacacc gggtgcattg ttgctggcgc
    39601 atacctcttc cactctcttt agtgaggaaa actgccagct actacaacac atagccgatc
    39661 gcatcgctat tgccgttggc aatgccgatg cctgccgtag aatgaccgat ttgcaggaaa
    39721 gtttgcagca agaaaaccac cagcttagcg agcagctcct ttcgaatctg ggcgtcggtg
    39781 acattatcta tcaaagccag gcaatggaag acctgctcca gcaggtagat attgtggcga
    39841 agagcgacag tacggtgttg atttgcggtg aaaccggaac cggcaaagag gtgatcgcca
    39901 gagcgatcca tcaacttagc ccgcgacacg acaagccgct ggtcaaaatc aactgcgctg
    39961 ccatccccgc cagtcttctg gaaagtgagt tattcggtca tgacaaaggg gcgtttactg
    40021 gtgcgattaa tacccatcgt ggtcgttttg aaattgccga tggcggcacg ttgtttctcg
    40081 atgaaattgg cgatctgccg ttagaacttc agcctaaact gctgcgcgta ttgcaggaac
    40141 gggagattga gcgtctcggc gggagtagaa cgatcccggt aaatgtcaga gtcattgccg
    40201 ccaccaaccg tgatttgtgg caaatggttg aagatcgcca gtttcgcagc gatctctttt
    40261 atcgcctgaa tgtcttccca ctggaattgc ctccgctgcg cgaccgtccg gaagatatcc
    40321 ctcttttagc aaagcatttc acgcaaaaaa tggcgcgcca tatgaatcgt tcaattgacg
    40381 ccatcccgac cgaggcgcta cgccagttga tgtcgtggga ctggccgggc aacgtgcgcg
    40441 agctggaaaa cgtgattgag cgagcggtac tgctgactcg cggtaacagt ctgaatttac
    40501 atcttaatgt ccgacaaagc cgtttactgc cgacgctaaa tgaagattca gcgcttcgca
    40561 gttcaatggc gtagttgctg cacccgacga cgccagagaa tgacgaagaa gaacgtcagc
    40621 gcattgttca ggtattgcga gaaaccaatg gcattgttgc cgggccccgt ggcgcggcga
    40681 cacgattagg gatgaagcgc accacgctgc tgtcacgaat gcagcgtctg ggaatctcgg
    40741 ttcgcgaggt gttgtaatct gcttttgcag gagtatgcat gagaaacaaa ctctctttcg
    40801 acttgcagtt gagcgccaga aaagcggcaa tcgctgaacg gattgccgcc cataaaattg
    40861 cccgcagtaa agtgtcggtc tttttaatgg cgatgtccgc tggcgtgttt atggcgatgt
    40921 ccgctggcgt gtttatggcg atcggattta ctttttacct ttccgttatc gccgatgccc
    40981 cgtcttcata ggcattaacc catctggtgg gcggcctttg ctttacactt ggctttattt
    41041 tgctggcggt ttgcggcacc agcctgttca cctcgtcggt aatgacggta atggcaaaaa
    41101 gtcggggcgt tattagttgg cgaacttggc tgattaacgc acttctggtg gcctgcggta
    41161 atctggcagg tattgcctgt ttcagtttgt taatctggtt ttccgggctg gtgatgagtg
    41221 aaaacgcgat gtggggagtc gcgattttac actgcgccga gggcaaaatg catcatacat
    41281 ttactgaatc tgtcagcctc ggcattatgt gcaatctgat ggtttgcctg gcgctgtgga
    41341 tgagttattg cggacgttcg ttatgcgaca aaatcgtcgc catgattttg tccatcaccc
    41401 tgtttgtcgc cagtggcttt gagcactgta tcgccaattt gtttgtgatt ccgttcgcca
    41461 ttgccattcg ccatttcgcc cctacctcct tctggcaact ggcgcacagt agcgcagaca
    41521 attttccggc actgacggtc agccatttta ttaccgccaa tctgctcccg gtgatgctgg
    41581 gtaatattat cggcggtgca gtgctggtga gtatttgtta tcgggctatt tatttacgtc
    41641 aggaatcctg aaaaatcagc ccggcgaaac agttcgtcgg gctatttatt tacgtcagga
    41701 atcctgaaaa atcagcccgg cgaaacagtt cgtcgggctg aagattattc ttgcgcgatt
    41761 tgcccatcgg gccaggcgtg gatcacggct ttgatcagcg tcgccaatgg aatggcgaag
    41821 aatacgcccc agaatcccca caaaccaccg aagatcacca ccgataaaat aatcaccagc
    41881 ggatgcaggt taaccgcttc ggagaacaac accggtacta gcaggttgcc gtccagcgcc
    41941 tgaataatca gatacaccgc gaagcagctc cagaattcag tacctgcgcc aaactggaat
    42001 agcgccacgc caaccaccgg aatggtcacc acaaatgcgc caatgtacgg aataagaacc
    42061 gagaagccga ccagcactgc cagcagcagc gaatagttca gcccgaagag caagaacccc
    42121 agccaggtgg cgatccccac cacgatcatc tccagcactt tgccgcggat atagttggtg
    42181 atttgttgat tcatctcctt ccacacctgt cctgccagcc cacggttacg cggcagcacc
    42241 cggcgaacgg cgttcagcat ctgctctttg tctttcagca ggaagaagac catcaatggc
    42301 accagcacca gatagacggc tatggtcagc aaaccgacca gcgaggcgag ggaaattttc
    42361 accaccgaat cgcccatggt cagcatccga ctgcgcatat tttcggccat tgcatcaata
    42421 atgcccgcgt ccattaacac cggatagcgg cgcggcaacg tggcggcaaa gtcagaaagc
    42481 ttattgagca tccccggcat atcgcggatt aagtagatgc cctgttgcca ggcgataggc
    42541 agtaccacga acgccatcag tagcaatata ccgacaaaaa ccaccaatac aatcgacgtc
    42601 gcccagcggc gggagcagcc aatagattgc aggcgcacgg ttggccattc cagcaaataa
    42661 gccagcacaa tagccaccag caacggagca agcaggccac taaagaaaaa gataatgcca
    42721 aatccggcaa ctaaaataac cagcaaggca atcgcttccg ggtcgctaaa acggcggcga
    42781 taccattgca tcaacatttc gagcataaag accttccctg aacctcaaga gcgggattgc
    42841 gatccgcaat tgtatcgaaa tgtcacaaaa aagacttcgc tttttatgac ggattcagga
    42901 aactgaaaag tcatttgagt gggctaatct tcgccgttac actcaaaggc ggcgaggtgg
    42961 gaacgatatt tcacagtatc ggtcaaatga ctatcttcag aaatacagga tagaggttat
    43021 gttcaggcag ttgaaaaaaa acctggttgc aaccctcatt gctgctatga ccattggtca
    43081 ggtagccccg gcgtttgccg acagcgcaga caccttgccg gatatgggaa cctccgcagg
    43141 aagcacgctt tccattggtc aggaaatgca gatgggcgac tattatgtcc gccagctacg
    43201 cggcagcgcg ccgttaatta atgacccgct gttaacgcaa tatattaatt cgctggggat
    43261 gcgtctggtt tcgcatgcca attcggttaa gacaccgttt catttttttc tgatcaacaa
    43321 cgacgaaatt aacgcctttg ctttctttgg cggcaacgtg gtgctgcact ctgccctgtt
    43381 ccgttattcc gataacgaaa gtcaactggc ttcagttatg gcgcacgaaa tctcccacgt
    43441 cacccaacgt cacctggcgc gagcgatgga agatcaacaa cgcagcgcgc cgctgacctg
    43501 ggtcggcgcg ttaggttcta ttttactggc gatggccagt ccgcaggcgg ggatggcggc
    43561 gctgaccggt acactggcgg gaacgcgcca ggggatgatc agtttcaccc agcaaaatga
    43621 acaggaagcg gaccgcattg gtattcaggt gctgcaacgc tcgggattcg atccacaggc
    43681 gatgccgacc ttcctggaaa aattgctcga tcaggcgcgt tactcctcgc gcccgccgga
    43741 aattttattg actcacccgt tgccggaaag tcgtctggca gatgcccgca accgtgctaa
    43801 tcagatgcgc ccgatggtgg tgcagtcgtc ggaagatttc tatctggcga aagcgcgcac
    43861 actggggatg tataattccg gacgtaacca gctcaccagt gatttgctgg atgaatgggc
    43921 gaaaggaaac gttcgtcagc aacgagcggc gcaatatggt cgtgctttac aggcgatgga
    43981 agccaataaa tacgacgagg cgcgtaaaac gctgcaaccg ttactggcgg cagaacctgg
    44041 caacgcatgg tatctcgatc tggctaccga tatcgatctt gggcaaaaca aagccaatga
    44101 ggcaatcaat cgcctgaaaa atgcccgcga tttacgcacc aatccggtgt tgcagctcaa
    44161 cctggcgaac gcttatctgc aaggcggtca accacaagaa gcggccaata ttctgaatcg
    44221 ctacaccttt aataataaag atgacagcaa cggctgggat ttgctggcac aggcggaagc
    44281 cgcgctaaat aaccgcgatc aggagctggc tgcgcgagca gaaggttatg cgctcgccgg
    44341 acgactcgat caggccattt cgctgttgag tagcgccagt tcgcaggtga aattaggcag
    44401 cctgcaacaa gcgcgttacg atgcgcgcat cgaccagttg cgccagctgc agaaacgctt
    44461 taagccttat accaagatgt aattcgcatt aaaggaagaa caatgaccaa acaggtaaaa
    44521 atctaccata acccacgctg ttcaaagagc cgggaaacgc tgaacctgct gaaagaaaac
    44581 ggcgtggagc cagaagtggt gctctacctt gagacacccg ccgatgcggc aacgctgcgc
    44641 gatttgctga aaatgctggg gatgaatagc gcccgtgaat tgatgcgcca gaaagaggat
    44701 ctttataaag aactaaacct ggctgacagt tcgcttagcg aagaggcgct gattcaggca
    44761 atggtggata atccgaagct gatggagcgc ccgattatgg tcgcgaatgg caaagcgcgg
    44821 attggtcgtc caccggagca ggtactggaa atcgtgggtt aagatgtgct ccatcgctag
    44881 ttgaagcaca ttgccggatg cgacgcttgc cacgtcttat ccggcctacg aatactatgg
    44941 attaacttcg cgtaggtcag gcgttcgcgc cgcatccgac aataaacacc ttatctacaa
    45001 cttcagaatt tctttcacaa acggaatggt cagcttacgt tgcgcggtaa tcgacgcacg
    45061 atccaactga tccaacgtca taaatagcgt gcgcatttct ctgtcgagcc gcttcagcaa
    45121 gaaacgcccc acatcttccg gcagttcaaa accacgcaaa cgcgcgcgta actgtagcgc
    45181 ctgcaactta tcttcatcag aaagtggctg caatttgtag atctgccccc agtcgagacg
    45241 cgacgcgaga tccggtaatc ccagattcaa ctgccgcggt ggacgatcgc cggtgatcaa
    45301 caaccgtgtt ttgcccgatt ccagaattcg attgtagaga tcgaaaatcg ccatctccca
    45361 caactcatcg cctgcaatac actcaatgtt gtcgatacag accagcgaca aatgctccat
    45421 accgtcgagc acttccggaa caaaccaggt gcgtttatcc agcgggacat agcccaccgc
    45481 atcgccacgc tgcgacaatt ccgcgcaagc cgcgtgcagc agatggctgc gccccgcgcc
    45541 ttcgcgtgcc cagagataga tgtaaccgct atgttcctga cgcagcacgt tttgcagcgc
    45601 ggccagtaaa gaggagttat cccccggcca gaaacttgca aaggtttcgt cgtcaggaag
    45661 ataaagtggc aaagagagct gtgccggtgt gttcagagat acctcaacca ggatttcaca
    45721 aaatcgcgag aagtttacca cagaatccca tgatgtttga accgggcagc aacactgccc
    45781 ggtcggtaca ttatttgtct gttatatccg cgtcttctgc gtccagcacc acttcttcag
    45841 ggcgcagcac actgatcagt ttgaaaatca aacttaagcc gataccgacg atggtcgcca
    45901 gcgccatacc ttttaactcc gccgcgccga tgtttacctt cgcgccactg acgccgatga
    45961 tcaaaatcac ggaagtcagg atcaggttct gtgctttgtt gtaatccact ttcgattcga
    46021 tcaaaacacg aataccggaa gcaccaatga caccataaag cagcagcgaa acgccgccca
    46081 tcaccggcaa tgggatcatc tggatagcgg cagccagttt accgacgcag gaaagcagga
    46141 tagcgaaaat cgccgccccg ccgataaccc aggtactgta aacacgggtg atcgccatca
    46201 cgccaatgtt ttctccgtaa gtggtatttg gcgtagagcc aaagaagccg gagatcacgg
    46261 tcgacaggcc gttagcaaac atcgaacggt gcagacctgg atcgcgcagc agatcttttt
    46321 tgacgatatt agccgttact accaggtgcc ctacgtgttc ggcaataacc actaacgccg
    46381 ctggcagaat agtcagaatg gcaaaccact cgaagcgcgg cgtatagagg gttggcagtg
    46441 caaaccagtg agcattaata atcggcgtgg tatcgacaat tcccattgcg aaagagagcg
    46501 cgtaccccac cagcacgcca attaaaatcg ggataattgc caggaaacca cgaaacagca
    46561 cggaacctaa aaccgtgacc gccagggtgg taatagagat gatgatggtt ttggagtttg
    46621 gcgtttgccc ttcagccggg agtaaacccg ccataccggc agctacgccc gccagctcca
    46681 gaccgatgac ggcaacgatt gcgcccattg ccgcaggtgg aaacagcacg tccaaccagc
    46741 cggtccccgc tttcttcacg ataaaagaaa ccaggcagaa cagcacgccg cacataataa
    46801 agccgcccag cgcgacttca taccctaacg gcaacagtaa caataccggt gaaataaagg
    46861 caaagctgga accaagataa gccggaattt tccctttaca gatgaagaga tacagcagcg
    46921 ttccaatacc gttaaataac agtacagtcg ccgggttaat atgaaataag acgggcacca
    46981 ggacggttgc accaaacatg gcgaacaaat gttgcaaact aagcgggatt gtctgtaaaa
    47041 gtggcggtct ttcactcacc ccgatagcac ggcgcgtcat agtattatcc tctgtattat
    47101 gtgttatagg cgctttactc aaaaaaaagc cgactcttaa agtcggcttt aattattttt
    47161 attctttatt tcgtaccaaa gattttgtca ccggcatcgc cgaggcccgg aataatgtat
    47221 ccgtgctcgt tcagtccctg gtcaatcgat gcagtataca gttcgacgtc cgggtgcgct
    47281 ttttccagcg cagcgatacc ttctggcgca gcgaccagca ccagaacttt gatgctgctg
    47341 cagcccgctt ttttcagcag gtcgatggtc gcgataacgg aaccaccggt tgccagcatt
    47401 gggtcaacga tcagcgccat acgctcatcg atgttagaaa ccagtttctg gaagtacggt
    47461 accggctcca gcgtttcttc attacggtac ataccgacaa cgctgatgcg cgcgctcgga
    47521 acgttttcca gcacaccgtc catcatacca agacccgcac gcagaattgg cacaacggta
    47581 attttcttac ctttgatctg gtcgatttct accgggccgt tccagccttc gatagttact
    47641 ttttccgttt cgaggtcggc ggtcgcttcg taagtcagca ggctacccac ttcggaagcg
    47701 agttcgcgaa agcgcttggt gctgatatct tgctcacgca tcagtcccag cttgtgtttg
    47761 acgagtgggt gtttgacttc cacgatcttc atactctttc tcctttgagg ggcagccaca
    47821 aaaaaatcga cggattatac ctcctttctt cagggcggca atattatttt cgttgacttt
    47881 agtcaaaatg ataacagttt gagataaagt tattttatat tcaaatggtt atgaaagaag
    47941 attattccat ccgaaaacta acctttgccc tggcacaagt cttctttcgc cgcgcgcccg
    48001 gggaaaagac gtgcaaaaag gttgtgtaaa acagtctcgc aaacgtttgc tttccctgtt
    48061 agaattgcgc cgaattttat ttttctaccg caagtaacgc gtggggaccc aagcagtgac
    48121 cgataaaacc tctcttagct acaaagatgc cggtgttgat attgacgcgg gtaatgctct
    48181 ggttggaaga atcaaaggcg tagtgaagaa aacgcgtcgt ccggaagtga tgggcggtct
    48241 gggcggcttc ggtgcgctgt gtgcattgcc gcaaaaatat cgtgaacccg tgctggtttc
    48301 cggcactgac ggcgtaggta ccaagctgcg tctggcaatg gacttaaaac gtcacgacac
    48361 cattggtatt gatctggtcg ccatgtgcgt taatgacctg gtggtgcaag gtgcggaacc
    48421 gctgtttttc ctcgactatt acgcaaccgg aaaactggat gttgataccg cttcagcggt
    48481 gatcagcggc atcgcggaag gttgtctgca atcgggctgt tctctggtgg gtggcgaaac
    48541 ggcagaaatg ccggggatgt atcacggtga agattacgat gtcgcgggtt tctgcgtggg
    48601 cgtggtagaa aaatcagaaa tcatcgacgg ctctaaagtc agcgacggcg atgtgctgat
    48661 tgcactcggt tccagcggtc cgcactcgaa cggctattcg ctggtgcgca aaattcttga
    48721 agtcagcggt tgtgatccgc aaaccaccga acttgatggt aagccattag ccgatcatct
    48781 gctggcaccg acccgcattt acgtgaagtc agtgctggag ttgattgaaa aggtcgatgt
    48841 gcatgccatt gcgcacctga ccggcggcgg cttctgggaa aacattccgc gcgtattgcc
    48901 agataatacc caggcagtga ttgatgaatc ttcctggcag tggccggaag tgttcaactg
    48961 gctgcaaacg gcaggtaacg ttgagcgcca tgaaatgtat cgcaccttca actgcggcgt
    49021 cgggatgatt atcgccctgc ctgctccgga agtggacaaa gccctcgccc tgctaaatgc
    49081 caacggtgaa aacgcgtgga aaatcggtat catcaaagcc tctgattccg aacaacgcgt
    49141 ggttatcgaa taatgaatat tgtggtgctt atttccggca acggaagtaa tttacaggca
    49201 attattgacg cctgtaaaac caacaaaatt aaaggcaccg tacgggcagt tttcagcaat
    49261 aaggccgacg cgttcggcct tgaacgcgcc cgccaggcgg gtattgcaac gcatacgctc
    49321 atcgccagcg cgtttgacag tcgtgaagcc tatgaccggg agttgattca tgaaatcgac
    49381 atgtacgcac ccgatgtggt cgtgctggct ggttttatgc gcattctcag cccggcgttt
    49441 gtctcccact atgccgggcg tttgctgaac attcaccctt ctctgctgcc gaaatatccc
    49501 ggattacaca cccatcgtca ggcgctggaa aatggcgatg aagagcacgg tacatcggtg
    49561 catttcgtca ccgatgaact ggacggtggc ccggttattt tacaggcgaa agtcccggta
    49621 tttgctggtg atacggaaga tgacatcacc gcccgcgtgc aaacccagga acacgctatt
    49681 tatccactgg tgattagctg gtttgccgat ggtcgtctga aaatgcacga aaacgccgcg
    49741 tggctggatg gtcaacgtct gccgccgcag ggctacgctg ccgacgagta atgcccccgt
    49801 agttaaagcg ccagctctgc cgctggcgtt tttcaattca cctgtaaatc gcaagctcca
    49861 gcagtttttt tccccctttt ctggcatagt tggacatctg ccaatattgc tcgccataat
    49921 atccaggcag tgtcccgtga ataaaacgga gtaaaagtgg taatgggtca ggaaaagcta
    49981 tacatcgaaa aagagctcag ttggttatcg ttcaatgaac gcgtgcttca ggaagcggcg
    50041 gacaaatcta acccgctgat tgaaaggatg cgtttcctgg ggatctattc caataacctt
    50101 gatgagttct ataaagtccg cttcgctgaa ctgaagcgac gcatcattat tagcgaagaa
    50161 caaggctcca acgctcattc ccgccattta ctgggcaaaa ttcagtcccg ggtgctgaaa
    50221 gccgatcagg aattcgacgg cctctacaac gagctattgc tggagatggc gcgcaaccag
    50281 atcttcctga ttaatgaacg ccagctctcc gtcaatcaac aaaactggct gcgtcattat
    50341 tttaagcagt atctgcgtca gcacattacg ccgattttaa tcaatcctga cactgactta
    50401 gtgcagttcc tgaaagatga ttacacctat ctggcggtgg aaattatccg tagcgatagc
    50461 atccgttacg cgctgctgga gatcccatca gataaagtgc cgcgctttgt gaatttaccg
    50521 ccagaagcgc cgcgtcgacg caagccgatg attcttctgg ataacattct gcgttactgc
    50581 cttgatgata ttttcaaagg cttctttgat tatgacgcgc tgaatgccta ttcaatgaag
    50641 atgacccgcg atgccgaata cgatttagtg catgagatgg aagccagcct gatggagttg
    50701 atgtcttcca gtctcaagca gcgtttaact gctgagccgg tgcgttttgt ttatcagcgc
    50761 gatatgccca atgcgctggt tgaagtgtta cgcgaaaaac tgactatttc ccgctacgac
    50821 tccatcgtcc ccggcggtcg ttatcataat tttaaagact ttattaattt ccccaatgtc
    50881 ggcaaagcca atctggtgaa caaaccactg ccgcgtttac gccatatttg gtttgataaa
    50941 gcccagttcc gcaatggttt tgatgccatt cgcgaacgcg atgtgttgct ctattatcct
    51001 tatcacacct ttgagcatgt gctggaactg ctgcgtcagg cttcgttcga cccgagcgta
    51061 ctggcgatta aaattaacat ttaccgcgtg gcgaaagatt cacgcatcat cgactcaatg
    51121 atccacgccg cacataacgg taagaaagtc actgtggtgg ttgagttaca ggcgcgtttc
    51181 gacgaagaag ccaacattca ctgggcgaag cgcctgaccg aagcaggcgt gcacgttatc
    51241 ttctctgcgc cggggctgaa aattcacgcc aaactgttcc tgatttcacg taaagaaaac
    51301 ggtgaagtgg tccgttacgc acacatcggg accgggaact ttaacgaaaa aaccgcgcgt
    51361 ctttatactg actattcgtt gctgaccgca gatgcgcgta tcaccaacga agtacggcgg
    51421 gtatttaact ttattgaaaa cccataccgc ccggtgacat ttgattattt aatggtgtca
    51481 ccgcaaaact ctcgccgtct gttatatgaa atggtagacc gcgaaatcgc caacgcgcag
    51541 caagggctgc ccagtggtat caccctgaag ctaaataacc ttgtcgataa aggcctggtt
    51601 gatcgtctgt atgcggcctc cagctccggc gtaccggtta atctgctggt tcgcggaatg
    51661 tgttcgctga tccccaatct ggaaggcatt agcgacaaca ttcgtgccat cagtattgtt
    51721 gaccgttacc ttgaacatga ccgggtttat atttttgaaa atggcggcga taaaaaggtc
    51781 tacctttctt ccgccgactg gatgacgcgc aatattgatt atcgtattga agtggcgaca
    51841 ccgctgctcg atccgcgcct gaagcagcgg gtgctggaca tcatcgacat attgttcagc
    51901 gatacggtca aagcacgtta tatcgataaa gaactcagta atcgctacgt tccccgcggc
    51961 aatcgccgca aagtacgggc gcagttggcg atttacgact acatcaaatc actcgaacaa
    52021 cctgaataac cctatgccaa tacacgataa atcccctcgt ccgcaggagt ttgctgcggt
    52081 cgatcttggt tcaaacagtt ttcacatggt catagcccgt gtggtagatg gtgccatgca
    52141 gattattggc cgcctgaaac agcgggtgca tctggcggac ggcctggggc cagataatat
    52201 gttgagtgaa gaggcaatga cgcgcggttt gaactgtctg tcgctgtttg ccgaacggct
    52261 acaagggttt tctcctgcca gcgtctgtat agtcggcacc catacgctgc gtcaggcgct
    52321 gaacgccact gactttctga aacgcgcgga aaaggtcatt ccctacccga ttgaaattat
    52381 ttccggtaat gaagaagccc gtctgatttt tatgggcgtg gaacataccc aaccggaaaa
    52441 aggtcgcaaa ctggttattg atattggcgg cggatctacg gaactggtga ttggtgaaaa
    52501 tttcgaacct attctcgttg aaagccgccg gatgggttgt gtcagctttg cccagcttta
    52561 ttttcctggc ggggtcatca ataaagagaa ttttcagcgc gctcgcatgg cggcagcaca
    52621 aaaactggaa actttaacct ggcaattccg tattcagggc tggaacgttg caatgggcgc
    52681 ttccggtacc ataaaagccg cccatgaagt gttaatggaa atgggcgaga aagacgggat
    52741 aattaccccg gaacgtctgg aaaaactggt aaaagaaatt ttacgtcacc gtaatttcgc
    52801 atcgctgagt ttaccgggtc tttccgaaga gcggaaaaca gtcttcgttc ctggactggc
    52861 gattttatgt ggtgtgtttg atgctttagc catccgtgaa ctgcgccttt ctgacggggc
    52921 gttacgcgaa ggcgtactgt atgaaatgga aggacgtttc cgtcatcagg atgtgcgtag
    52981 tcgcaccgcc agcagcctcg ccaaccagta tcacatagac agcgaacagg cccgacgagt
    53041 gctggatacc actatgcaaa tgtacgaaca gtggcgggaa cagcaaccga agctggcgca
    53101 tccgcaactg gaggcgctac tgcgatgggc cgccatgctg catgaggtcg ggttgaatat
    53161 caaccacagc ggtttgcatc gccactccgc ttatattctg caaaacagtg acttgccggg
    53221 ttttaatcag gaacagcagc tgatgatggc gacactggtg cgctatcacc gtaaagcgat
    53281 taagctcgac gatctgccgc gctttacctt gtttaagaag aaacagttcc tgccactgat
    53341 acagctattg cgccttggcg tattactaaa caatcaacgt caggcaacca ccacaccgcc
    53401 aacattgaca ctgattactg atgacagtca ctggacactg cgtttcccgc atgactggtt
    53461 tagtcagaat gcgctggtac tgcttgatct ggaaaaggag caagaatact gggaaggcgt
    53521 ggctggctgg cggttgaaaa ttgaagaaga aagtacccca gaaatcgccg cttaatgctt
    53581 tgcgggccga cgagaaatgt cggcccgcat tattcaggca ctttcgcgaa tgggttcgat
    53641 ttcattcagc gtatcaatta acggttgcgg cttaccaata agataaccct gcatataatc
    53701 gatccccaaa gagagcaccg cctcgcggat ctcttcgttt tcaacgtact ctgccactac
    53761 ccgcattttc ttcattcgcg ccagatggca aatcgacgcc acaatctgat aatccagact
    53821 atttgacaca atattgcgga taaagctgcc gtcaatttta agcagatcgg cattcacatt
    53881 tttaagccgt gcatagctgg cgtagccggt gccgaaatca tcaatcgcaa tctggcagcc
    53941 taattcctga agatgctgca aggtgatttg cgcctgctta acattggtca gagcattact
    54001 ttcggtgact tcaaaaataa gttgccacgc ttcaatctga tatttagcca gcaactgact
    54061 gacttcaaca ggaaaacgag cctgacatac cgaggttgga gacagattaa tagcaaaacg
    54121 gtgagcgggc atcttcgctc tgttttcagc cataaattgc agcgtatgct caatgaccca
    54181 catgtcgata ctcgacgata aaccaaattc gtgcgcgacc ggcaagaagc taccggggcc
    54241 gatcagttca tcattctcac ctttcatgcg cagaagaatt tcatggtaaa catcaccacg
    54301 cataccggta atcggctggg ccatcaggaa aaaatggttg tgttccagcg cctgctgtag
    54361 tcgattcatc atcgcgactt tatctttcaa ttcgcgttgc aaatacattg ccccgcaacg
    54421 ctgcatattt tccggggcgt tggtcacgat ggaaagttcg gcgaccgtat ttagctctcc
    54481 cagcagcagg tagatatgat tcactggcga gcgcacatag cagtaactga cgccaatctg
    54541 cggttgcatc ggcatcccat cccaaaagaa acgaaattgc ttgagatgac tatccagtgc
    54601 ggtaatgcgc tcctggtgcg attctgtatt cagtcgcagc gcgagatcgt tacccgaaag
    54661 ctgataaaca tcttcacccg gttccagcaa gggtgacagc cagtgagaaa gtttttgctt
    54721 gt
//
LOCUS       DAZPCS010000011        54466 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZPCS010000011 DAZPCS010000000
VERSION     DAZPCS010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 54466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54466
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.011"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            533..1822
                     /gene="sdaC"
                     /locus_tag="ACHUQ7_000608"
     CDS             533..1822
                     /gene="sdaC"
                     /locus_tag="ACHUQ7_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFR1134158.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            1880..3247
                     /gene="sdaB"
                     /locus_tag="ACHUQ7_000609"
     CDS             1880..3247
                     /gene="sdaB"
                     /locus_tag="ACHUQ7_000609"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFR1134159.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            3358..4113
                     /gene="xni"
                     /locus_tag="ACHUQ7_000610"
     CDS             3358..4113
                     /gene="xni"
                     /locus_tag="ACHUQ7_000610"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFR1134160.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKADRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(4166..5314)
                     /gene="fucO"
                     /locus_tag="ACHUQ7_000611"
     CDS             complement(4166..5314)
                     /gene="fucO"
                     /locus_tag="ACHUQ7_000611"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFR1134161.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(5342..5989)
                     /gene="fucA"
                     /locus_tag="ACHUQ7_000612"
     CDS             complement(5342..5989)
                     /gene="fucA"
                     /locus_tag="ACHUQ7_000612"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFR1134162.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            6536..7852
                     /gene="fucP"
                     /locus_tag="ACHUQ7_000613"
     CDS             6536..7852
                     /gene="fucP"
                     /locus_tag="ACHUQ7_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFR1134163.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAATPFVTVLGPESSSHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEILGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            7885..9660
                     /gene="fucI"
                     /locus_tag="ACHUQ7_000614"
     CDS             7885..9660
                     /gene="fucI"
                     /locus_tag="ACHUQ7_000614"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFR1134164.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            9769..11188
                     /gene="fucK"
                     /locus_tag="ACHUQ7_000615"
                     /pseudo
     CDS             9769..11188
                     /gene="fucK"
                     /locus_tag="ACHUQ7_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            11190..11612
                     /gene="fucU"
                     /locus_tag="ACHUQ7_000616"
     CDS             11190..11612
                     /gene="fucU"
                     /locus_tag="ACHUQ7_000616"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFR1134165.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMEPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            11670..12401
                     /gene="fucR"
                     /locus_tag="ACHUQ7_000617"
     CDS             11670..12401
                     /gene="fucR"
                     /locus_tag="ACHUQ7_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFR1134166.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(12445..13545)
                     /gene="rlmM"
                     /locus_tag="ACHUQ7_000618"
     CDS             complement(12445..13545)
                     /gene="rlmM"
                     /locus_tag="ACHUQ7_000618"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFR1134167.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(13538..13933)
                     /locus_tag="ACHUQ7_000619"
     CDS             complement(13538..13933)
                     /locus_tag="ACHUQ7_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFR1134168.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(13952..14869)
                     /gene="gcvA"
                     /locus_tag="ACHUQ7_000620"
     CDS             complement(13952..14869)
                     /gene="gcvA"
                     /locus_tag="ACHUQ7_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFR1134169.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGFNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(15220..15447)
                     /locus_tag="ACHUQ7_000621"
     CDS             complement(15220..15447)
                     /locus_tag="ACHUQ7_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFR1134170.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDIGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            15639..16844
                     /gene="csdA"
                     /locus_tag="ACHUQ7_000622"
     CDS             15639..16844
                     /gene="csdA"
                     /locus_tag="ACHUQ7_000622"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFR1134171.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGTVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            16844..17287
                     /gene="csdE"
                     /locus_tag="ACHUQ7_000623"
     CDS             16844..17287
                     /gene="csdE"
                     /locus_tag="ACHUQ7_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFR1134172.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGNSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(17338..18144)
                     /gene="tcdA"
                     /locus_tag="ACHUQ7_000624"
     CDS             complement(17338..18144)
                     /gene="tcdA"
                     /locus_tag="ACHUQ7_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFR1134173.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(18382..19479)
                     /gene="mltA"
                     /locus_tag="ACHUQ7_000625"
     CDS             complement(18382..19479)
                     /gene="mltA"
                     /locus_tag="ACHUQ7_000625"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFR1134174.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            19688..19764
                     /locus_tag="ACHUQ7_000626"
     tRNA            19688..19764
                     /locus_tag="ACHUQ7_000626"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19722..19724,aa:Met,seq:cat)
     gene            complement(19839..21092)
                     /gene="amiC"
                     /locus_tag="ACHUQ7_000627"
     CDS             complement(19839..21092)
                     /gene="amiC"
                     /locus_tag="ACHUQ7_000627"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFR1134175.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEEEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            21324..22655
                     /gene="argA"
                     /locus_tag="ACHUQ7_000628"
     CDS             21324..22655
                     /gene="argA"
                     /locus_tag="ACHUQ7_000628"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFR1134176.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     LHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(22717..24543)
                     /gene="recD"
                     /locus_tag="ACHUQ7_000629"
     CDS             complement(22717..24543)
                     /gene="recD"
                     /locus_tag="ACHUQ7_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFR1134177.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASNPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHTIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(24543..28085)
                     /gene="recB"
                     /locus_tag="ACHUQ7_000630"
     CDS             complement(24543..28085)
                     /gene="recB"
                     /locus_tag="ACHUQ7_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFR1134178.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEPPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLLITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDTFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(28078..30966)
                     /gene="ptrA"
                     /locus_tag="ACHUQ7_000631"
     CDS             complement(28078..30966)
                     /gene="ptrA"
                     /locus_tag="ACHUQ7_000631"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFR1134179.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMINANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(31142..34510)
                     /gene="recC"
                     /locus_tag="ACHUQ7_000632"
     CDS             complement(31142..34510)
                     /gene="recC"
                     /locus_tag="ACHUQ7_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFR1134180.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNSLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FHTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(34523..34873)
                     /locus_tag="ACHUQ7_000633"
     CDS             complement(34523..34873)
                     /locus_tag="ACHUQ7_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFR1134181.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVLMVLMVLMVMIVTALSGIQR
                     TSMNSLASRNQYQKLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGG
                     REGEMTRLHCPNCQ"
     gene            complement(34858..35265)
                     /locus_tag="ACHUQ7_000634"
     CDS             complement(34858..35265)
                     /locus_tag="ACHUQ7_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFR1134182.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(35262..35825)
                     /locus_tag="ACHUQ7_000635"
     CDS             complement(35262..35825)
                     /locus_tag="ACHUQ7_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFR1134183.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(35816..36286)
                     /locus_tag="ACHUQ7_000636"
                     /pseudo
     CDS             complement(35816..36286)
                     /locus_tag="ACHUQ7_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(36470..37264)
                     /gene="thyA"
                     /locus_tag="ACHUQ7_000637"
     CDS             complement(36470..37264)
                     /gene="thyA"
                     /locus_tag="ACHUQ7_000637"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFR1134184.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGVKAPVAI"
     gene            complement(37271..38146)
                     /gene="lgt"
                     /locus_tag="ACHUQ7_000638"
     CDS             complement(37271..38146)
                     /gene="lgt"
                     /locus_tag="ACHUQ7_000638"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFR1134185.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(38297..40543)
                     /gene="ptsP"
                     /locus_tag="ACHUQ7_000639"
     CDS             complement(38297..40543)
                     /gene="ptsP"
                     /locus_tag="ACHUQ7_000639"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFR1134186.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSCIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(40556..41086)
                     /gene="rppH"
                     /locus_tag="ACHUQ7_000640"
     CDS             complement(40556..41086)
                     /gene="rppH"
                     /locus_tag="ACHUQ7_000640"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFR1134187.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(41439..41585)
                     /gene="ygdT"
                     /locus_tag="ACHUQ7_000641"
     CDS             complement(41439..41585)
                     /gene="ygdT"
                     /locus_tag="ACHUQ7_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFR1134188.1"
                     /translation="MLSTESWDNCEKPPLLFSFTALTCDETPVFSGSVLNLVAHSVDK
                     HGIG"
     gene            41771..42460
                     /gene="mutH"
                     /locus_tag="ACHUQ7_000642"
     CDS             41771..42460
                     /gene="mutH"
                     /locus_tag="ACHUQ7_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFR1134189.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALDGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVARLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            42529..43242
                     /locus_tag="ACHUQ7_000643"
     CDS             42529..43242
                     /locus_tag="ACHUQ7_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFR1134190.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            43380..43598
                     /gene="ygdR"
                     /locus_tag="ACHUQ7_000644"
     CDS             43380..43598
                     /gene="ygdR"
                     /locus_tag="ACHUQ7_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFR1134191.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            43706..44746
                     /locus_tag="ACHUQ7_000645"
     CDS             43706..44746
                     /locus_tag="ACHUQ7_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFR1134192.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(44779..45969)
                     /gene="lplT"
                     /locus_tag="ACHUQ7_000646"
     CDS             complement(44779..45969)
                     /gene="lplT"
                     /locus_tag="ACHUQ7_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFR1134193.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASAIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAARPGQSWNLINMTRSFLNACTSLWCNGETR
                     FSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKLV
                     TLETVSRCMPAGILIGVVVPIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERGK
                     KSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWIW
                     QRRH"
     gene            complement(45962..48121)
                     /gene="aas"
                     /locus_tag="ACHUQ7_000647"
     CDS             complement(45962..48121)
                     /gene="aas"
                     /locus_tag="ACHUQ7_000647"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFR1134194.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            48707..49738
                     /gene="galR"
                     /locus_tag="ACHUQ7_000648"
     CDS             48707..49738
                     /gene="galR"
                     /locus_tag="ACHUQ7_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFR1134195.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(49745..51007)
                     /gene="lysA"
                     /locus_tag="ACHUQ7_000649"
     CDS             complement(49745..51007)
                     /gene="lysA"
                     /locus_tag="ACHUQ7_000649"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFR1134196.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDTLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            51129..52064
                     /gene="lysR"
                     /locus_tag="ACHUQ7_000650"
     CDS             51129..52064
                     /gene="lysR"
                     /locus_tag="ACHUQ7_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFR1134197.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPTGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGISVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(52051..52743)
                     /gene="ygeA"
                     /locus_tag="ACHUQ7_000651"
     CDS             complement(52051..52743)
                     /gene="ygeA"
                     /locus_tag="ACHUQ7_000651"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFR1134198.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(52872..54290)
                     /gene="araE"
                     /locus_tag="ACHUQ7_000652"
     CDS             complement(52872..54290)
                     /gene="araE"
                     /locus_tag="ACHUQ7_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFR1134199.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGAAFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
BASE COUNT        13317 a        14736 c        13424 g        12989 t
ORIGIN      
        1 ttgccgggtt ttctcgtttt tggtcactta ctcatcaact catttcattt gttatatgaa
       61 tgtttcttac caccctcacg cgacaaatat catcacagtt aatatgtcat acaatttatg
      121 ttgcaacaca aacgtttccc tattttcata aaccgtattt tatcgctatg aaaaagaaat
      181 tatcgccatg attaactaaa agtattgatt ttttcagttc aacctacata tattgcgcgc
      241 cccggaagaa gtcagatgtc gtttaatggg caaatattgc ccttaaattc tcttttactt
      301 ttgatttaca gagtaaagcg ttgggataat ctatcttcca agcagattat tgtatttggg
      361 atcaagatca ctgatagata cataacttgt gtgtatcttt ccgccctcaa attattacgg
      421 cggtaaatga ttaagccatc gccgatagac agatttcatt tttacggtca ggcaccttcc
      481 cgggctgaac tggctaaaag ctgaattatt tgcattcctc caggagaaat agatggaaac
      541 gactcaaacc agcacgattg cgtcgaaaga ctctcgtagt gcctggcgca agacagacac
      601 catgtggatg ctgggccttt acggcacggc aatcggcgcg ggcgtgctgt tcctgccaat
      661 caacgccggt gttggcggta tgatcccgct gatcatcatg gctatccttg cgttcccgat
      721 gacgtttttt gctcaccgcg gcctgactcg cttcgtactg tctggtaaaa acccgggcga
      781 agacatcacc gaggttgtag aagaacactt tggtattggc gcaggtaaac tgattaccct
      841 gctctacttc ttcgctatct acccgatcct gctggtttat agcgtggcaa tcaccaatac
      901 cgttgaaagc ttcatgtctc accagctggg tatgacgcca ccgccgcgtg cgattctgtc
      961 gctgatcctg atcgtgggta tgatgaccat cgttcgcttt ggtgagcaga tgatcgttaa
     1021 agcgatgagt attctggtat tcccgtttgt tggcgtactg atgctgctgg ctctgtacct
     1081 gatcccgcag tggaacggcg cagcactgga aacgctgtct ctggacactg catctgcaac
     1141 cggaaacggt ctgtggatga ccctgtggct ggcaattccg gtaatggtgt tctcgttcaa
     1201 ccactctccg atcatctctt ctttcgccgt tgcgaagcgt gaagagtacg gcgatatggc
     1261 agaacagaaa tgctcgaaga tcctggcatt cgcacacatc atgatggtgc tgaccgtaat
     1321 gttcttcgtc ttcagctgcg tactgagcct gactccggca gacctggctg cggctaaaga
     1381 gcagaacatc tcgattctgt cttacctggc taaccacttt aacgcaccgg ttatcgcgtg
     1441 gatggctccg attatcgcga ttatcgctat caccaaatcc ttcctcggcc actacctggg
     1501 cgcacgtgaa ggcttcaacg gtatggtgat taaatctctg cgtggtaaag gtaagtctat
     1561 cgaaatcaac aagctgaacc gtatcactgc gctgttcatg ctggtaacga cctggattgt
     1621 tgccaccctg aacccgagca tcctgggtat gattgaaacc ctgggcggcc caatcatcgc
     1681 gatgatcctg ttcctgatgc cgatgtacgc aattcagaaa gtaccggcaa tgcgtaagta
     1741 cagcggtcac atcagcaacg tattcgttgt cgtgatgggt ctgattgcaa tctccgcaat
     1801 cttctactct ctgttcagct aagtcctttc gcgccgcttt cgggcggcgc ttcctccgtt
     1861 ttaacgcgat gtatttccta tgattagcgt attcgatatt ttcaaaatcg gcattggccc
     1921 ttccagttct cataccgttg gaccaatgaa agcgggtaaa caatttaccg acgatctgat
     1981 tgcccgtaac ctgcttaaag acgtgacccg cgtggtggtt gacgtgtacg gctcgctctc
     2041 tctgactggt aaaggccacc acacagatat cgccattatt atgggcctgg cgggtaacct
     2101 gccggatacc gtggatatcg attccatccc cggttttatt caggatgtga atactcatgg
     2161 tcgcctgatg ctggcaaacg gtcagcatga agtggagttc ccggttgatc agtgcatgaa
     2221 cttccatgca gacaaccttt ctctgcatga aaacggtatg cgcattaccg cgctggcggg
     2281 cgataaagtc gtttacagcc agacttacta ctctattggc ggtggcttta tcgttgatga
     2341 agagcatttt ggccagcagg atagcgcacc ggttgaagtt ccttatccgt acagttcagc
     2401 agccgatctg caaaaacatt gtcaggaaac cgggctgtca ctctctggcc tgatgatgaa
     2461 aaacgagctg gcgctgcaca gtaaagaaga gctggaacag cacctggcga acgtctggga
     2521 agtcatgcgt ggcggtattg agcgcggtat ttccaccgaa ggcgtgttgc ctggcaaact
     2581 gcgcgttcca cgccgtgctg cggcactacg ccggatgctg gtcagccagg ataaaaccac
     2641 cactgacccg atggcggttg ttgactggat caacatgttt gcactggcag tgaacgaaga
     2701 gaacgctgct ggcggtcgcg tggtgactgc gccgactaac ggtgcgtgcg ggattatccc
     2761 ggctgtgctg gcgtactacg ataagtttat ccgcgaagtg aacgctaact cactggctcg
     2821 ttacctgctg gtagccagcg ccattggttc tctttataag atgaacgcgt cgatttctgg
     2881 tgccgaagtg ggttgccagg gtgaagttgg cgtggcgtgc tctatggcgg cagctggtct
     2941 ggcagaactg ttaggcgcaa gcccggcgca ggtttgcatc gcagcggaaa tcgcgatgga
     3001 gcacaacctc ggtctgactt gtgacccggt cgcggggcag gttcaggtac cgtgcattga
     3061 gcgtaacgcc attgcagcag tgaaagcggt gaacgccgcg cgtatggcac tgcgccgtac
     3121 cagcgaaccg cgcgtctgcc tcgataaagt tatcgaaacc atgtacgaaa caggtaaaga
     3181 catgaacgcc aagtaccgcg aaacctctcg cggcggcctg gcgatgaaga tcgttgcctg
     3241 cgattaatct ctcccagagg cctcgttttg cgaagcctct tcctgttttc tcaaccagcc
     3301 atagcctgtt ccggcatcga atgttaccct tatcgcctga tctttaaggg ggttatcgtg
     3361 gctgttcatt tgcttattgt cgatgcactg aatcttatcc gtcgcattca tgccgttcag
     3421 ggttcgccct gtgtcgaaac ctgccagcat gcgctcgatc agctcattat gcacagccag
     3481 ccaacccacg cggtcgccgt ttttgatgat gaaaaccgca gtagcggctg gcgtcatcag
     3541 cgtttaccag attacaaagc ggatcgaccg ccaatgccgg aagagttgca cgacgagatg
     3601 cctgcattac gcgccgcctt tgagcaacgc ggcgtcccgt gctggtcagc cagcggcaac
     3661 gaagccgatg acttagccgc cacactggcg gtcaaagtga cacaggccgg gcatcaggca
     3721 acgattgttt cgacagataa aggctactgt cagttacttt caccgacatt acgtattcgt
     3781 gattacttcc agaaacgttg gctggatgcg ccatttatcg ataaagaatt tggcgttcaa
     3841 ccgcagcagt tgcccgatta ctggggactt gcggggatca gcagttcaaa ggtaccgggt
     3901 gttgcgggaa tcggaccaaa aagcgccacg cagctgctgg tcgagtttca gagtctggaa
     3961 gggatatatg agaatctgga tgcggttgcc gaaaagtggc gcaaaaaatt agaaacccat
     4021 aaagagatgg cgtttctgtg ccgcgatatt gcccgcttac aaaccgattt gcatatcgac
     4081 ggcaatttac agcaattgcg gttggtacgg taacggcgag ccggatacgc cgcaaatcgt
     4141 atccggcatt atcacatcag cgcatttacc aggcggtatg gtaaagctct acaatatcct
     4201 caagcgttgc ttcacgcggg ttgccaccgg tacaaacatc atccagtgcc gcctgagcca
     4261 gtgccgaaat gtcttccttg cgtacaccaa catcacgcaa atgtggcgga ataccgacat
     4321 cacggttgag ggcaaacacc gcttcaacag cggcattacg cgccccttcc aggctcatac
     4381 cttccacttt cacgcccata acgcgcgcga tatcgcggta cttctcaccg gtaaagtcag
     4441 cgttatagcg catgacatgc ggtaacagga tggcgttcgc aacaccgtgt ggagtgttat
     4501 aaaacgcgcc cagtggatgc gccataccat gcaccaaccc taacccaaca ttcgagaagc
     4561 ccatacccgc aacatactgc ccgagcgcca tttcttctcc ggcatcctta tcaccagcaa
     4621 ccgatcctcg cagcgcccca gcaatgattt caatcgcttt aatgtgcagt gcatcggtta
     4681 gtgcccacgc gccacgggta atatacccct caatagcatg agtgagcgca tcaacacccg
     4741 tcgcggcttt cagcgccgga ggcataccat ccatcatgtc agcatcaata aacgccacct
     4801 gcgggatatc atgcggatca acgcaaacaa acttgcgccg tttttcttcg tcagtgatca
     4861 cgtagttaat ggtcacttct gccgcagtac ctgctgtggt ggggattgcc agaatcggta
     4921 cactgggttt attggtcggg gaaagccctt ccaggctacg cacatcggca aactccgggt
     4981 tgttgctgat aatgccaatt gctttacaag tatcctgtgg agaaccacca ccaatagcga
     5041 tcaggtaatc cgcgccgcta ttctggaata caccgagccc ttctttgacg acagtaattg
     5101 ttgggttggg cactacgccg tcgtaaatcg cccatgccag ccctgcagca tccatcttat
     5161 cggtcacttt cgccaccacg ccgcattgca ccagcgtttt atcggtgacg atcagcgcct
     5221 tctgataacc acggcgtttc acctcatcgg ttaaagcccc aacagcaccc cgaccaaacc
     5281 atgccgtttc gttcagaatc attctgttag ccatcatcct tctccttgtt gctttacgaa
     5341 attactcttc aatccgtaac ccataggttt tgaatttctc cagcactacg gcaatctctt
     5401 catcgctcag cactggcacc gggtccgtaa tcgccagggt cgtcaggtaa agttgcgcca
     5461 gcacttcaac ttcatgcgcc agccataacg ctttttccag attcacctca caagcgataa
     5521 gcccatgatg ttgtaacaaa gttgccttac gatttttgag agccagcgca acatgttcag
     5581 aaagttcgcg tgttccaaag gtcgcataag gcgcgcaagg aatagaatta ccgccagccg
     5641 ccgcaatcat gtagtgaata gcggggatcg atcggttaag aatggaaact gccgtgcaat
     5701 gaacggcatg attgtgaaca accgcgttgg catccggtct gctttgatag gctgccatat
     5761 ggaaacgcca ttcgcttgag gggagctttc cttcctcatg tttaccgttg ccatcaataa
     5821 agacaatatg cgactccgtc agtttttcat atggaatgcc tgtaggcgta atcagcatcc
     5881 catcctgata acgtacactg acgttccccg ctgtcccctg gttcagtccc aggcgggtca
     5941 tttccaggca agtgtcaata atctgacgag caagtttatt tcgttccatt agctacctct
     6001 ctctgattca aaacagggca ataatgttgt tcctttcaca ctattgaatt agccgtttaa
     6061 ttacccacca tcttcttcct gattaacaag aaagaaattc acaagcttat attttgtgac
     6121 ctagctcaac taatcacagt aaataactgc aagttctctt tttataaccc cgttaaaaat
     6181 gaccgctctt aaaaatattt atcaaaacgg tcatttttcc atttcccctc gccagaaatg
     6241 cccgttttcg gcacaaacaa ttaatacggt catctggttt gtgtttttta tgatttattt
     6301 tctgaaacgg gcatgaaatt tcgattatta aagtgatggt agtcacataa agtcaccttc
     6361 cagctaataa gtgtgaccgc cgtcatatta cagagcgttt tttatttgaa aatgaatcca
     6421 tgagttcatt tcagacaggc aaatattcac cgatatgaag cccgaactcg ctggttttgc
     6481 acttttgaaa acataaccga ttacgtgctt aagcttctga acctaagagg atgctatggg
     6541 aaacacatca atacaaacgc agagttaccg tgcggtagat aaagatgcag ggcaaagcag
     6601 aagttacatt attccattcg cgctgctgtg ctcactgttt tttctttggg cggtagccaa
     6661 taaccttaac gacattttat tacctcaatt ccagcaggct tttacgctga caaatttcca
     6721 ggctggcctg atccaatcgg ccttttactt tggttatttc attatcccaa tccctgctgg
     6781 gatattgatg aaaaaactca gttataaagc agggattatt accggattat ttttatatgc
     6841 cttgggcgct gcattattct ggcccgctgc agaaataatg aactacacct tatttttagt
     6901 tggcctattt attattgcag ccggattagg ttgtctggaa actgccgcaa ccccttttgt
     6961 tacggtatta gggccggaaa gcagtagtca cttccgctta aatcttgcgc aaacatttaa
     7021 ctcgtttggc gcaattatcg cggttgtctt tgggcaaagt cttattttgt ctaacgtgcc
     7081 acatcaatcg caagacgttc tcgataaaat gtctccagag caattgagtg cgtataaaca
     7141 cagcctggta ttatcggtac agacacctta tatgatcatc gtggctatcg tgttactggt
     7201 cgccctgctg atcatgctga cgaaattccc ggcattgcag agtgataatc acagtgacgc
     7261 caaacaagga tcgttctccg catcgctttc tcgtctggcg cgtattcgtc actggcgctg
     7321 ggcggtatta gcgcaattct gctatgtcgg cgcacaaacg gcctgctgga gctatttgat
     7381 tcgctacgct gtagaagaaa ttctaggtat gactgcaggc tttgccgcta actatttaac
     7441 cggaaccatg gtgtgcttct ttattggtcg tttcaccggt acctggctca tcagtcgctt
     7501 cgcaccacac aaagtcctgg ccgcctacgc attaatcgct atggcactgt gcctgatctc
     7561 agccttcgct ggcggtcatg tgggcttaat agccctgact ttatgcagcg cctttatgtc
     7621 gattcagtac ccaacaatct tctcgctggg cattaagaat ctcggccagg acaccaaata
     7681 cggttcgtcc ttcatcgtta tgaccattat tggcggcggt attgtcactc cggtcatggg
     7741 ttttgtcagt gacgcggcgg gcaacatccc cactgctgaa ctgatccccg cactctgctt
     7801 cgcggtcatc tttatctttg cccgtttccg ttctcaaacg gcaactaact gaacatattt
     7861 tccgaataaa gtgaggaatc tgtaatgaaa aaaatcagct taccgaaaat tggtatccgc
     7921 ccggttattg acggtcgtcg catgggtgtt cgtgagtcgc ttgaagaaca aacaatgaat
     7981 atggcgaaag ctacggccgc actgctgacc gagaaactgc gccatgcctg cggagctgcc
     8041 gtcgagtgtg tcatttccga tacctgtatc gcgggtatgg ctgaagccgc tgcttgcgaa
     8101 gaaaaattca gcagccagaa tgtaggcctc accattacgg taacgccttg ctggtgctat
     8161 ggcagtgaaa ccatcgacat ggatccaacc cgcccgaaag ccatttgggg ctttaacggc
     8221 actgaacgcc ccggcgctgt ttacctggct gcggctctgg cagctcacag ccagaaaggc
     8281 atcccggcat tctccattta cggtcatgac gttcaggatg ccgatgacac atcgattcct
     8341 gccgatgttg aagaaaaact gctgcgcttt gcccgcgccg gtttggccgt cgccagcatg
     8401 aaaggtaaaa gctatctgtc gctgggcggc gtttcgatgg gtatcgccgg ttctattgtt
     8461 gatcacaact tctttgaatc ctggctggga atgaaagtcc aggcggtgga tatgaccgaa
     8521 ctgcgtcgcc gtatcgatca gaagatttac gacgaagccg aactggaaat ggcactggcc
     8581 tgggctgata aaaacttccg ctatggcgaa gatgaaaata acaaacagta tcaacgcaat
     8641 gccgagcaaa gccgcgcagt tctgcgcgaa agtttactga tggcgatgtg tatccgcgac
     8701 atgatgcaag gcaacagcaa actggccgat attggtcgcg tggaagaatc acttggctac
     8761 aacgccatcg ctgcgggctt ccaggggcaa cgtcactgga ccgatcaata tcccaatggt
     8821 gacaccgccg aagcgatcct caacagttca tttgactgga atggcgtgcg cgaacccttt
     8881 gtcgtggcga ccgaaaacga cagtcttaac ggcgtggcaa tgctaatggg tcaccagctc
     8941 accggcaccg ctcaggtatt tgccgatgtg cgtacctact ggtcaccaga agcaattgag
     9001 cgtgtaacgg ggcataaact ggacggactg gcagaacacg gcatcatcca tttgatcaac
     9061 tcaggttctg ctgcgctgga cggttcctgt aaacaacgcg acagcgaagg taacccgacg
     9121 atgaagccgc actgggaaat ctctcagcaa gaggctgacg cttgcctcgc cgctaccgaa
     9181 tggtgcccgg cgatccacga atacttccgt ggcggcggtt actcttcccg cttccttacc
     9241 gaaggcggcg tcccgttcac catgactcgt gtcaacatca tcaaaggcct gggaccggta
     9301 ctgcaaatcg cggaaggctg gagcgtggaa ttgccgaagg atgtgcatga catcctcaac
     9361 aaacgcacca actcaacctg gccaaccacc tggtttgcac cgcgcctcac tggtaaaggg
     9421 ccgtttacgg atgtgtattc ggtaatggcg aactggggtg ccaaccacgg agttctgacc
     9481 atcggccacg ttggcgcaga ctttatcacc cttgcctcca tgctgcgtat cccggtatgt
     9541 atgcacaacg ttgaagagac caaagtgtat cgtccttctg cctgggctgc gcacggcatg
     9601 gatattgaag gccaggatta ccgcgcttgc cagaactacg gtccgttgta caagcgttaa
     9661 tacattacac cctgatgtga tgcccggtcg ctccggctac cgggcctgaa caagcaagag
     9721 tggttagccg gataaacaat gttatccggc tatattgcag gagcgattat gaaacaagaa
     9781 gttatcctgg tactcgactg tggcgcgacc aatgtcaggg ccatcgcggt taatcggcag
     9841 ggaaaaattg ttgcccgcgc ctcaacgcct aatgccagcg atatcgcgat ggaaaacaac
     9901 acctggcacc agtggtcttt agactgccat tttgcaacgc tttgctgatt gctgtcggca
     9961 aatcaatagt gaactgactg aatgccacat ccgcggtatc gccgtcacca cctttagtgt
    10021 ggatggcgct ctggtagata agcaaggcaa tctgctctat ccgatcatta gctggaaatg
    10081 tccgcgaaca gcagcggtta tggacaatat tgaacggtta atctccgcac agcggttgca
    10141 ggctatttct ggcgtcggag cctttagttt caatacgtta tataagttgg tgtggttgaa
    10201 agaaaatcat ccacaactgc tggaacgcgc gcacgcctgg ctcttcattt cgtcgctgat
    10261 taaccaccgt ttaaccagcg aattcactac tgatatcacg atggccggaa ccagccagat
    10321 gctggatatc cagcaacgcg atttcagtcc gcaaatttta caagccaccg gtattccacg
    10381 ccgactcttc cctcgtctgg tggaagcggg tgaacagatt ggtacgctac agaacaacgc
    10441 cgcagcaatg ctcggcttac ccgttggcat accggtgatt tccgcaggtc acgataccca
    10501 gttcgccctt tttggcgctg gtgccgaaca aaatgaaccc gtgctctcct ccggtacatg
    10561 ggaaatttta atggttcgca gcgcctaggt tgatacttcg ctgttaagtc agtacgccgg
    10621 ttccacctgc gaactagata gccaggcatg gttgtataac ccaggtatgc aatggctggc
    10681 atccggcgtg ctggaatggg tgagaaaact gttctggacg gctgaaacac cctggcaaat
    10741 gttgattgaa gaagctcgtc tgatcgcacc tggcgcggat ggtgtgaaaa tgcagtgtga
    10801 tttattgtcg tgtcagaacg ctggctggca aggagtgacg cttaatacca cgcgggggca
    10861 tttctatcgc gcggcgctgg aagggttaac tgcgcaatta cagcgcaatc tacagatgct
    10921 ggaaaaaatc gggcacttta aagcctatga attattgtta gtcggtggag gaagtcgcaa
    10981 cacattgtgg aatcagatta aagccaatat gcttgatatt ccggtaaaag ttctcgacga
    11041 tgccgaaacg accgtcgcag gagctgcgct gttcggttgg tatggcgtag gggaatttaa
    11101 cagcccggaa gaagcccgtg cacagattca ttatcagtac cgttatttct acccgcaaac
    11161 tgaacctgaa tttatagagg aagtgtgaaa tgctgaaaac aatttcgccg ttaatttctc
    11221 ccgaactatt gaaagtgctg gcagagatgg gacatggaga tgaaattatt ttttccgatg
    11281 ctcactttcc cgcccattcg atggaaccgc aggtgatccg cgctgatggc ctgttggtga
    11341 gcgacttgct ccaggcgatt atcccgttat ttgagctgga cagttatgcc ccgccgctgg
    11401 tgatgatggc ggcggtagag ggtgacactc tcgatcctga agtagaacga cgttaccgta
    11461 atgcgctttc actacaagcc ccgtgtcctg acatcatccg catcaatcgt tttgcgtttt
    11521 atgaacgggc gcaaaaagcc tttgcgatcg ttatcacagg cgaacgagcg aagtacggga
    11581 atattctttt aaaaaaaggg gtaacaccgt aatctcatac cggtacgccc gcatgacgcg
    11641 ggcggttatc gaatgatggg gtgaaaaata tgaaagcggc acgccagcaa gcgatagtcg
    11701 acctgctgct gaaccatacc agcctgacca cggaagctct ctctgaacag ctaaaggtca
    11761 gtaaagaaac cattcgtcgc gatctcaatg aattacagac gcagggtaaa attctgcgca
    11821 atcatggacg cgctaaatat atccaccgtc aaaatcaaga cagtggcgat ccctttcaca
    11881 tcaggctgaa aagccattat gcgcataaag cagatatcgc gcgcgaggcg ctcgcgtgga
    11941 ttgaagaagg gatggtgata gccttagacg ccagttcaac ttgctggtat ctggcacgcc
    12001 agttgcctga catcaacatt caggtcttca ccaatagcca tccgatttgc catgaactcg
    12061 gtaaacgcga acgcattcaa ctgatcagtt ccggcggcac acttgagcgc aaatatggct
    12121 gttacgtcaa tccctcgctg atttcccaac ttaaatcgct ggaaatcgat ctgtttattt
    12181 tttcttgtga agggatcgat agcagcggcg cactgtggga ctccaatgcg atcaacgctg
    12241 attacaaatc gatgctatta aaacgtgccg cgcaatcgtt gttattgatt gataaaagta
    12301 aatttaatcg ttcaggggaa gcccgcatcg ggcatctgga tgaggtaacg cacattattt
    12361 ctgatgagcg ccaggttgca acttctttgg taacagcctg acggaaaggg tagcaggccg
    12421 gagacgacgc cccggccttg cctgttatcg ctcgtcgcga cgaccaccca ccgcagccca
    12481 gatgcggcgg acgtgcaccg tcacttcttc gcgatcgtga tacaactgcc gcgcctgaat
    12541 ctgagcattt atgccatgtt catcaagctg tgcctgaata tacgccagat tgtgtgatac
    12601 ttcttcgtag cgttttttca tcggcagttt gagattgaaa atggtttcac ggcaccagcc
    12661 attaaccagc cactgcgcca tcaatgccgc aactttagcc ggtttttcaa ccatatcgca
    12721 taccatccag gagatattgc tgcgtgtcgg acggaatttg aaaccgtctt cccgcagcca
    12781 cgtcacctgc ccggtatcca tcagactttg cgccatcggg ccgttgtcga cggaataaac
    12841 ccacatgttg cgcttcacca gttggtaggt ccagccgcca gggcaagcgc ctaaatccac
    12901 cgcccacatc ccgttcgcca ggcgttcatc ccactcatcc gcaggaataa acacatgaaa
    12961 tgcctcttcc agtttgagcg tggaacgact cggcgcctct gccggaaatt tcaggcgcgg
    13021 aatgcccata tagaacggcg aattattgtt gctgtatgag taaccggtat agcagcatcc
    13081 tggtgcaata aagaatacat gcacaaccgg acgcttcggc gtttcatagt tcgccagcac
    13141 cccagcatcg cgcaaggcag cgcgtagcgg aacagtgaac ttacggcaga atttcagcaa
    13201 ctctttgctt tcgttggtat cggcaacttc aacacgcagt tcaccgccct tctctactac
    13261 accctgtaac atgccgacaa tgggggtaat acgatcttct ggcggcaaat gctgcaggag
    13321 ttcccccacc acaaaccact ggcgggcaaa aattaatgaa ctgaacggca gctcacggat
    13381 taacttatcg ccatcatcag gttgataaca ttcataaatg acataacccg cattctcttt
    13441 cacgcgggca aaaccgaaaa tttcccgctg gccggcttta tcggtaattt ctgcggcgca
    13501 ctctttttca aagcccggac ggcacagcaa tacaacctta ttcatgactt acgcccttgc
    13561 gctttaaacg gatagcacca actaacatta acgcccagcc cgcaaggaag ctcacgccgc
    13621 caaccggtgt gacaaacgcc cacaaacgca gatgggacag cgccaggcaa taaaggctgc
    13681 cgctgaacag caccgtgcct aacgcgagga aaacgctact ccagtaaaac cagatactga
    13741 tgcgacgctg cattgccacc gccagaccta agatcgccag cgtatgaaac gcctggtatt
    13801 cgagaccggt ttggatccag cccatctcaa cggcccccat ggttttactt aacacatgcg
    13861 caccaaaagc gcccagtgcc acaaaaatga agccgctaat ggcggcgaaa atcagcataa
    13921 aacggctggt catggtcgta ccctacgtaa attattgttc ataacgaaag cggaattttt
    13981 cttgttcagc agcggctttc gccaggatcc attggcgaaa ggcggctatt ttacccagtt
    14041 ctgcctggct gtcatgacaa accagataaa aagcattttt actgaccaga acatcattaa
    14101 acgggcaaac aagacgtccg gcctcgattt cagattgcgc catcacgtta tttgccagcg
    14161 ccactccctg cccgtgaata gccgcttgca gcaccatggc gctatgacta aaaattggcc
    14221 cttgctgaac gttgatatga ttaaacccca actgtcgggt atatgtctgc cagtcacggc
    14281 gtgacgcatc atgtaataac gtatgtttag ccagatcttc cggggtcttc aagggttttt
    14341 cgcctgtcag cagtagcggc gaacacaccg gcaataaata ttcggcgtac agtttttcca
    14401 cccgtagccc cggccagttg ccccgaccat aaaatatcgc cacatcaaca tcatccgcca
    14461 gcttatcttc ctgacgatca accgcctgga ttcgaacgtc aattcccgga taagctgaat
    14521 taaagctgga aagtcgcgga accaaccaat gaatggcgaa actggggagt aaactgaccg
    14581 tcaacgcccc cttggcgcta cgggcctgga gtttacgcgt cgcttcggtt aattgcgaaa
    14641 atatctcttt gatatcgagg aaatagcttt gcccttcctc ggtcagcagg agtgaacgat
    14701 tacggcggcg gaacagtttt agccccaaaa aatcctcaag agacttgatt tgatgactta
    14761 ctgcggcctg ggtcacaaaa agctcttctg ctgcgcgagt aaaacttaaa tggcgtgctg
    14821 cggcatcaaa aactcgtaag gcatttagcg gtggtaatcg tttagacatg gcttttaaac
    14881 tttgatgtta aatgaattta acaattagat cacactgtgt aacctattag tttttttaat
    14941 ctgagccatt ataaattgtc cgttgagctt ctaccagcaa atacctatag tggcggcact
    15001 tcctgagccg gaacgaaaag ttttatcgga atgcgtattc tggtgaactt ttggcttacg
    15061 gttgtgatgt tgtgttgttg tgtttgcaat tggtctgcga ttcagaccat ggtagcaaag
    15121 ctaccttttt tcacttcctg tacatttacc ctgtctgtcc atagtgatta atgtagcacc
    15181 gcctaattgc ggtgcttttt tttaccttgc gatcgcgaat tactgttcca gttcgaccat
    15241 ctctttcacg tcagtacggt tgatctgctg tttgttgcca ttagcgtctt tatacgaaat
    15301 cataccgata tcgttatcag tctgtggttt gccgtcagag acgatggtac gtccgtcatt
    15361 ggtgtgcatc acatagttcg aaccggaaca ggcgctcagg gcaaaagtca gcatacaggc
    15421 agaaataatt gcggcagtct ttttcatcat cttctcctta aaacgattaa taaatcttaa
    15481 tagtattgat taacaggcta aaattaacgc ctaacactat tcagcatatg ttacttacgc
    15541 ggcttcgcca ggatatccag ataattctga tggttagcac tctccttgta tcaaagtgaa
    15601 ttttgcgtca cgatcggtgc atcaagccga ggagtaccat gaacgttttt aatcccgcgc
    15661 agtttcgcgc ccagtttccc gcactacagg atgcgggcgt ctatctcgac agcgccgcga
    15721 ccgcgcttaa acctgaagcc gtggttgaag ccactcgaca gttttacagc ctgagcgccg
    15781 gaaacgtcca tcgcagccag tttgccgaag cccaacgcct gaccgcgcgt tatgaagctg
    15841 cacgagagaa agtggcgcaa ttactgaatg caccggatga taaaactatc gtctggacgc
    15901 gcggcaccac tgaatccatc aacatggtgg cacaatgcta tgcgcgtccg cgtctgcaac
    15961 cgggcgatga gattattgtc agcgtggcag aacaccacgc taacctcgtc ccctggctga
    16021 tggtcgccca acaaactaga gccaaagtgg tgaaattgcc gcttaatgcg cagcgactgc
    16081 cggatgtcga tttgttgcca gagctgatta ctccccgtag tcggattctg gcgttgggtc
    16141 agatgtcgaa cgtgactggc ggttgcccgg atctggcgcg agcgattacc tttgctcatt
    16201 cagccgggat ggtggtgatg gttgatggtg ctcaggggac ggtgcatttc cccgcggatg
    16261 ttcagcaact ggatatcgat ttctatgctt tctctggtca caaactgtat ggcccgacgg
    16321 gtatcggcgt gctgtatggc aaatcagaac tgctggaagc gatgtcgccc tggctgggtg
    16381 gcggcaaaat gattcacgaa gtgagttttg acggtttcac cactcaatct gcgccgtgga
    16441 aactggaagc aggaacgcca aatgtcgctg gcgtcatagg attaagcgcg gcgctggaat
    16501 ggctggcaga ttacgatatc aaccaggccg aaagctggag ccgtagctta gcaacgcttg
    16561 cagaagaagc gctggcgaaa cgtccaggct ttcgttcatt ccgctgccag gattccagcc
    16621 tgctggcctt tgattttgct ggcgttcatc acagcgatat ggtgacgcta ctggcggagt
    16681 acggtattgc cttgcgggcc gggcaacatt gcgctcagcc gctactggca gaattaggcg
    16741 taaccggcac actgcgcgcc tcatttgcgc catataatac aaagagtgat gtggatgcgc
    16801 tggtgaatgc cgttgaccgc gcgctggaat tattggtgga ttaatgacaa acccgcaatt
    16861 cgccggacat ccgttcggca caaccgtaac cgcagaaacg ttacgcaata ccttcgcacc
    16921 gttgtcgcaa tgggaagata aatatcgcca gttgatcatg ctggggaaac agcttccggc
    16981 tttgccagac gagttaaaag cgcaggctaa agagattgcc ggatgcgaaa accgcgtctg
    17041 gctgggatat accgtggctg aaaacggcaa aatgcacttc tttggcaaca gcgaagggcg
    17101 cattgtgcgc ggcctgttgg cggtgttgtt gactgccgtt gaggggaaaa ccgccgccga
    17161 attacaggca cagtcgccgc tggcattgtt tgatgagctg ggattacgtg cgcagcttag
    17221 cgcctcacgc agccaggggt taaatgcgtt aagcgaggcg attatcgctg cgacgaagca
    17281 ggtttaagca cagaaccggg tcggataaga cgttcgcgtc tcatccgacc tgattgttta
    17341 accctgacgc gccgctttcg ccatcatctt cttcagcgca tgagaaaccg caacaaaccc
    17401 aaaggtggcg gtcaccatcg ttgccgcgcc aaatccagat gcacaatcca tccgcttcgg
    17461 cccttctgcc gtggctttca tcgcacacac cgtaccgtct gactgcgggt acaccagcgc
    17521 ttcagtagaa aacacgcaat ccacgccgag cttaccttta ctgtttttca ctacgccaaa
    17581 atcgcttttc aggcgctcgc gcaacttcgc cgccagcgga tcctgaatcg ttttcgccag
    17641 atcggtaacc tgaatctgcg tcgggtcaat ctgcccaccc gcgccaccgg tcgtgaccag
    17701 cgggatttta ttgcgccgac aataagcaat cagcgccgct ttgggccgta cactatcaat
    17761 ggcatcaatc acgtacgaat aaccggcact catatattgc gctacgttat ccggcgtcac
    17821 gaaatcatcc accaccgtta cacggcactc cgggttaatc tggcgaatgc gctccgccat
    17881 gacttccgct tttgccagcc cgacgttatc gcgcagagcg tgaatttgcc gattggtatt
    17941 ggtgacgcac acatcatcca tatcgataag cgtgattgcg ccaatccccg tgcgcgccag
    18001 cgcttccgcc gcccaggaac cgacaccacc gataccaacc acacaaatat gcgcgtcagc
    18061 aaacagttgc aacgcttttt caccatacag acgcgctgtg ccaccaaaac gctgacgcca
    18121 tgcatcacta attaccacag acatagcacc tcagaattaa aaagggcgaa gttatccctc
    18181 accctaaacg cataaatgcc tgatgcgcta cgcttatcag gcctacataa ctcctgcaat
    18241 attttggatt tgcatgattt gtaggccgga taaggcgttc acgccgcatc cggtatgaac
    18301 aacgcgcact tagtcaataa tcaggatgtg aggttacccc tcccctgtca tatccgtaaa
    18361 aacggcatac agaatatcac atcagccgct aaagacgtta cctgcgcccg gagccgtttt
    18421 cagcacccaa acccgtccat agtggttgta ccaacctgcg cggtgtccgg cttccggccc
    18481 gatcccttga tagatatcga agtgttggcc tttgattgcg cccccgacat ccaacgccac
    18541 catcagacgc aattcgtact gaccgttaaa tttgccgtta ttatccagca gcggcacttc
    18601 tgccagcaag gtagtacctg gcggaataat tgaacgatcg gaggcaacag aagcacggcc
    18661 aaccagcggc accgcacttg cccctttcac cggtgcaaaa gattgcggtt taaagaagac
    18721 gaaagacggg ttctgttcca gcagctcgcg aacctcggct tcactgtgtg tttcgcccca
    18781 atgacgaatc gcctgcatcg acatatcttc ttttttcact tcgccacggt cgatcagcac
    18841 cttaccaatg ctgcgatagg catgaccgtt tttccctgca tagctgaaaa agttaagcgg
    18901 actgccatca ccaaagtcga tatacccact accctgcaca tccataatga agttatccat
    18961 cagagagttg ctgtaagcga ggatgtattt atcgcccaat gccccggcgt agatctccgc
    19021 acgagacggc agacgaccac gttttggcgg catacggtaa ataggatact ggaactcgcc
    19081 ctggcgggta tggcgcgcct gaattaccgg cgtgtaatag cctgtaaact gcacgttacc
    19141 atagttgtcg gcaccttcca tctgccaggc atcaatgccg aactggcgca tattgcgggt
    19201 atcaccgcct gcgcgcagcc actcttgcac cgcgttataa acattactct ggttgccata
    19261 cagacgcggt gacgaattac ggatatggtt aatttgctcg gcaaaatcac cggcgttaat
    19321 cggcgcgcca acggcatctg gctggttcac cagagagaaa ggctgggtaa atttcccgtc
    19381 tttatattgc tgtccgcgat cggttggttt ggaagagcag gcggcaagca ttgccacaac
    19441 cgtgcccata agaaggtact ttacccaacg tcctttcatt gttctcttct tcaggttaaa
    19501 aaataaggcg caacgaagat aacaaaccgc cgcgtttaat gaaatgagca gcctctccct
    19561 gacgcaaatt ttgcacaaaa aacaggcttt agtggtttgt ttttgttcaa aatcatgcta
    19621 aatccgtgat cggggtaaaa aaaaggttgc atgaaaacgc gagcggagta tagtgcgcat
    19681 ccacggacgc ggggtggagc agcctggtag ctcgtcgggc tcataacccg aaggtcgtcg
    19741 gttcaaatcc ggcccccgca accaattatt gaacacccta acgggtgttt ttttgtttct
    19801 gatctcccat aaaaaaagcg ccattcagcg cctttttatc atccccttct cgccagcgtt
    19861 gccccatcgg caaaatacgc tttaatcccc gcaagaatag actccgcaac ttcctgctgg
    19921 aaagtcgccg ttttcagttt acgctcttcc tcaacgttac tgataaacgc cgtttcgacc
    19981 agaatggagg gaatatctgg tgcctttagt acggcaaacc cggcctgttc aacttgattt
    20041 ttatgcagct tgttgatttt tccgagctta ttcagtaccg ctttaccaaa cttaaggctg
    20101 tcagcaatag tcagcgactg caccatatcg aacatggtgt ggtcgacata gcggtcaccg
    20161 cttttgctca cgccaccaat caagtccgag gcgttctggg tttgtgccag atattttgcc
    20221 gcagtactgg ttgcgccttt ggttgagagc gcaaacaccg aggaaccgct cggctgacga
    20281 ctggtaaagg cgtcggcatg gatagagaca aacaggtcag cacgctgttt ctgggctttt
    20341 gctacgcgca cttgcaacgg aatgaagatg tcttcattgc gcgtcatgta caccttcata
    20401 ttgccctctt cctcgatcag agagcgcaaa cggcgagcta tttgcaatac cacatctttt
    20461 tcgcgcgttt tgtatttccc caccgcaccg gagtcttcgc caccgtggcc agggtcaagc
    20521 ataatgacaa tcggacgatc gcgccctgct ttacccggtt gtggaccact ttgcgctggc
    20581 ggcacctgct tttcgaggtc gcctttgttg taatcctcca gcagcgccag cagcgggtcc
    20641 tgcatatcct gtgcattggc cggatagagg tccatcacca gacgctcttt aaacccggcg
    20701 accggcgcaa gggcaaacag ctgcggtttt acgttttgct ttaattcaaa aaccatgcgt
    20761 acggtttgcg ggtcaaattg cccgacgcgc gccgacttga tgaacgggtc gtctgcacga
    20821 atttgtgccg ccatcccctt gagtaccgag ttcaggttta catcttcgat atccaccacc
    20881 acgcgttcag ggttactcaa cgcgaactgc ttatatttca gctgacgatt tgattctacc
    20941 gtcacgcggg tgtagctgga cgcaggccag acgcgcaccg ccacgacctg gctaaccgca
    21001 gccaggctga cctgacttac gctcaatagc cacatagcgc ccgcgccttg cagtaaacga
    21061 cgacggctga ttgcagtgtt ggatcctgac atgcctctcc cgagcaaaag aaatctaagc
    21121 tgtgtaacaa gtaaacgact aatttgaccg gaaactttag cgaaagacgc ataatctgtc
    21181 atctaataaa cggtaaacat tctttttata ttcacggcat tactgataaa aaagtcgctc
    21241 tcgcataaaa tttacacttg caccctgcga aaaaacagaa taaaaataca ataatttcaa
    21301 ataatcatgc aaaggggtgt gccgtggtaa aggaacgtaa aaccgagctg gtcgagggat
    21361 tccgccattc ggttccctat atcaataccc accggggaaa aacgtttgtc atcatgctcg
    21421 gcggtgaagc cattgagcat gagaatttct ccagtatcgt taatgatatc gggttgttgc
    21481 acagcctcgg catccgtctg gtggtggtct atggcgcacg tccgcagatc gacgcaaatc
    21541 tggctgcgca tcaccacgaa ccgctgtatc acaagaatat acgtgtgacc gacgccaaaa
    21601 cactggaact ggtgaagcag gctgcgggaa cattgcaact ggatattact gctcgcctgt
    21661 caatgagtct caataacacg ccgctgcagg gcgcgcatat caacgtcgtt agtggcaatt
    21721 ttattattgc ccagccgctg ggcgtcgatg acggcgtgga ttactgccat agcgggcgta
    21781 tccggcggat tgatgaagac gcgctccatc gtcaactgga aagcggtgca atagtgctaa
    21841 tggggccggt cgctgtttca gtcactggcg agagctttaa tctgacctcg gaagagattg
    21901 ccactcaact agccatcaaa ctgaaagctg aaaagatgat tggtttttgc tcttcccagg
    21961 gcgttactaa tgacgacggt gatattgtct ccgaactttt ccctaacgaa gcgcaagcac
    22021 gggtagaagc ccaggaagag aaaggcgatt acaactccgg tacggtgcgc tttttgcgtg
    22081 gcgcagtgaa agcctgccgc agcggcgtgc gccgctgtca tttaatcagt tatcaggaag
    22141 atggcgcgct gttgcaagag ttgttctcac gcgacggtat cggtacgcag attgtgatgg
    22201 aaagcgccga gcagattcgt cgcgcaacaa tcaacgatat tggcggtatt ctggagttga
    22261 ttcgcccgct ggagcagcaa ggtattctgg tgcgccgttc tcgcgagcag ctggagatgg
    22321 aaatcgacaa attcaccatt attcagcgcg ataacacgac tattgcctgc gccgcgctct
    22381 atccgttccc ggaagagaag attggggaaa tggcctgtgt ggcagttcac ccggattacc
    22441 gcagttcatc acggggcgag gttctgctgg aacgcattgc cgctcaggcg aagcagagcg
    22501 gcttaagcaa attgtttgtg ctgaccacgc gcagtattca ctggttccag gaacgtggat
    22561 ttaccccggt ggatattgat ttactgcccg agagcaaaaa gcagttgtac aactaccagc
    22621 gtaaatccaa agtgttgatg gcggatttag ggtaagcgat gaaaatcacc ggatgcggca
    22681 ggcgtaacac tcgtgcgtcg catccggcaa ttacgtttat tcccgcgaac taaacaacgc
    22741 cgccagacca ctgcgccgct cagtacgagt ggcgattgcc gcacttaata tacgctcatc
    22801 ggcatacagc gacagacgac gacgcgcgcg ggtcaccgcg gtgtatacca gctctcgcgt
    22861 tactaccggc gtgcgttggc tcggtaaaat caacgccgca tggtcgaact ccgatccctg
    22921 cgatttatgt accgtcatcg cccacgtagt ttcgtgctct ggcaagcgac tcggttgcac
    22981 agacttaata ttgccgtccg gcatcgcaaa ccagacgcgc gtcccctgcc cgcgatccag
    23041 cgcaataccg atatcgccat taaacaaccc aagcgcgctg tcattacggg caatcatcac
    23101 cggtcggcct tcgtaccaac gagagtgcgg atgacgatga attttgcgct tctgttgcat
    23161 aaactgttca attcgctcat tcagtccagc cacgccaaac ggcccttccc gcagggcgca
    23221 caaaagctgg tactcattga acgcctgaat gattaaatcc ggctcggcac gcgcttgcag
    23281 cagatccaga taacgtccgt aacccgcaag agcttcctca agcatcgcaa tataatcttc
    23341 gccgctctgt aaaagtcgtt tttcgatatc agtaaaatcc tgctgaaaaa cggttttcac
    23401 tgccgtttta tcaccacggt tgatcgccgc agctaactga ccaatgccag aatcgctgcc
    23461 gaaacgatag cttttttgca gcaggcagag actgtcgcgc aaagatgccg cttctgtgcc
    23521 ggttcctgcc ggaacgtgag tccccgtcag gcggcttagc tgcctggcac gctcggcggt
    23581 aaagcccgcg ttggcataag cgcagatatc gcccagcaca gccccagcct caaccgaggc
    23641 cagttgatca cgatcgccga gaaagatcac tcgcgcatga tctggcaagg cgtcgatcag
    23701 tctcgacatc ataggcagat cgatcattga cgcttcatct accaccagca catcaagatg
    23761 cagcgggtta ccggcatgat gacgtaaacg ctggctaccc ggctgcgcgc ccagcaatcg
    23821 gtgcaaagtg ctggcatctt ccggaatgcg tttcttttgt tcatcggtca gcggtaactg
    23881 tcgcaaagcc ttgccgagag attcggttaa gcgcgcggca gctttacccg ttggtgcagc
    23941 cagacggata cggcagcgtt cgccgtcggc catttgaatt aacgctgcca gcaacttcgc
    24001 cacggtggtc gttttaccgg tgcctgggcc gccggaaatc accgagatcc gccgcgtcag
    24061 cgccactgcc gctgcaactt tttgccagtt aatttcatcg cttactggaa aaagtttgtc
    24121 cagggtttgc gccagtagag cttcatcaac ctcaatggta tgattcacct cattgaagaa
    24181 gcgcgctacc gtccgctcgt tacaccacat gcgattcaaa taaagacgat cgccacagag
    24241 gatcatcggc gtgggttcat ctcccctgct gaccgcttga gaagccagca agcattcttc
    24301 ccaattttgt agctcaccga tttcactgac acaggtcgcc aacagcggat tcgacgcctc
    24361 gttattttcc agtcgtgaaa gcggcaaaca gacgtgtccc tctccggcat catgacttaa
    24421 cagtgccgcc gcgagggtga cggcaggatg ttcatctccc gccacggtca gggcaaattg
    24481 cacatccagc gggcgtagct gtttgtgctc cacagcttcc agtaattgct tttgcaattt
    24541 cattacgcct cctccagggt cataccggca aacatctcat ccatcagggc aatcaacccg
    24601 gcgttgggtc gggttgtgta aatcccttgt tgcggatgtt ctttatcaac gccacgcagg
    24661 aacagataga taacgccgcc aaagtgaaga tcatagtcgt aatcagcaat gcgatggcgc
    24721 agataacgat gcagcgccag ggtataaagc tgatattgca gatcatagcg gtgtgcctgc
    24781 atcgctgctg ccatagcctg ttgggtgtaa gccgaactgt cttcacccag ccagttggat
    24841 ttatagtcga gcaggtaata acgcccttcg tggcggaata ccaggtcgat aaagcctttt
    24901 aacatgccgc gtacctgcat gaactccagc ggcgggcagc cagcggatag cgggtcaaac
    24961 tggcgaatta gtgcatcaag ctggctggcg atgagcggtt cgctaatcgg caggtaaaac
    25021 tccatctcca cctgtttatc gcggtcggaa agctgactca ggctaacacc cgtttcattg
    25081 agaggtgcct ggaggacagc cgtgatccac tcggtcaaaa ccggttccca ctgcgattca
    25141 aagccgccga gttccagttt ttcctgtacc cagttcgggt caaccggctg ggtaaaatcg
    25201 aggtcttcaa acaaactgtg caagaatgtc cccggtgacg caccgcgcgg gaactgatgc
    25261 ggtgttaacg tcggttcttc aacgacgctg accacgcctg cggcatcaac atccagccgt
    25321 ggcatcagat cctgagcgat accgtgacca cgctgctgca agccggagta gctggtgaca
    25381 cgccagttat cgccgggcaa tcgttgtagt gtcctcgcat tcagttctgc agttaaagca
    25441 tcattaacct gccagggctg gttatcacca gtttgtgccg tttgccaggc aatatcatca
    25501 tcgcataacg cttcaataca ggtgcgaagc cctgccgcat cttgcggttc ccctttttgc
    25561 agcaaacgcc cgagcgcact ttggtggacg tcggtgtcac cttttttatc gccacgacgg
    25621 cgcaccagcg gtgcaacgcc gagactgcaa tgccaaaccg aacgcgtcag cgccacgtaa
    25681 agcaaacgca gatcttccgc cagacgttcg acctccgcga ggtcgacgct ttctggcgca
    25741 gcattaagat ccaaaactgc ctcaaacgag tggcgatcgt gataaaacgc ctgatcctgg
    25801 acgcggaaat tggtaataaa tggcaaccag accagcggat attccagccc tttcgattta
    25861 tggatcgtga caatctgcac cagatgttta tcactttcga ggcgcatttg ttggctggag
    25921 gcattactgt ctggctcgag gatatgttgc gataaccagc gtaccagcgc atgttcactt
    25981 tccagctgcg ttccggcttc ttgaagcagt tcgctgatat gcaagatatc ggtaagacgc
    26041 cgctcaccgc ctgccgttgc cagcaagttt tctgcaatgt tacgcgccga catcagcgcc
    26101 cgcagcatcg gcataacgcc acgtttgcgc cagatttgcc gataaccatc gaactcttcg
    26161 actaccgcat cccacgcatg ttcgtcatta ttcagcgttt caatatccag cgcattcagc
    26221 cccatcattg acgttgccag cgcactgcgc agggtgttct cacgttcggg cgtcatcacc
    26281 gcctgcaaca accaaagcat ttcctgcgct tccagagttt caaaaacact gtcgcggttc
    26341 gaaaggtaaa cggaagggat ttccagcaac gttaaggcat cgcgcacctg ggcggcctcc
    26401 tggcggctgc gcaccagcac gctgatgtcc gaagcacgca ccggacgcgc gtcgtcgccg
    26461 ttcatcagca acgcttcgcc ccgctgtccg gcttgtagcc agtcgcggat ttgcgcagca
    26521 catacctgcg ccatggtact ttgatagtcg ccaacgccgc agctttcgcc ttccatcagc
    26581 cacattttca tcgcaggctg cgtttcacct ttaaatacaa aacgtaacgc ctgatttttc
    26641 ccggctgatt tcactggaat aaacggtatt tcgcgaaaca tgaacgtgtc atcagtctgg
    26701 ctgaaaagct tgttcacact gttcaccatt ccaggtgcgg agcgccagtt ggtgtctaaa
    26761 gtgtagtggg catgaacttc actacgcgcc ttcatataag tgaagatatc cgcaccccgg
    26821 aatgcatata tggcctgctt cgggtcgcca atcagcaaca atgcggtttc cggctgatgg
    26881 tgccagatac ggcgaaaaat tcggtactgc tgggggtcgg tatcctgaaa ttcatcgatc
    26941 attgccaccg ggaatcgcgt acggatcgcc gctgccaaca cctcgccgct ttcgctacgc
    27001 agcgcggaat cgagccgact taacatgtca tcaaaaccca attcgccacg gcggcgtttt
    27061 tcacgcgcta ctgtttcgcg gatctcagcc aatgcgcggg tgatcaacag atcgcggatc
    27121 gacaatggtt ctgcaagcag ttgatcgatc gcctcaaaca gtggatgtcg cggggtttcc
    27181 cccccggcct tcgtgcgatc ttctaagaaa cgctgggaga atttttccag cgactccggc
    27241 aactgataac tgtttgtctc ttcttctgcc caggcgctga tcttttcgat ccatttagcc
    27301 tgattgctac ggttaaactt gcgtcgatca ataccagaag attcgatcag cgcatccagt
    27361 tcacccactg cgtcgcgcca ctgctgtttt accgtatcaa tacgcgccac aatttgcgcg
    27421 tggcgggaag ccagcgtttc atcatcgggc ggcggtgctt tgataaccgg cggttcgcct
    27481 tgcagataac gattaatatc gcgcagcaac gcctgcggcc ctttccaggt ttcaaagacg
    27541 acctgggcta tttcacgcgg cagcgggtag cagtggcgac gccagaaatc ggcgcaggcc
    27601 tggtagcgta gcagagactc atcttcaatc agctgctgct caaacagcat gccggattca
    27661 aaggcattca ggttgagcat gcgctggcaa aagccgtgaa tagtaaagac tgccgcttca
    27721 tccatctgcc gttcggctaa caacaaccac tgcgcggctt gcgctttatc gtcgatctct
    27781 tccagcaggc gttcgtacag tggattgtcg gtggtttcac gcagacaggc gatgcgcaac
    27841 tcgtggatat tgctacggat acgaccgcgc aattctgccg tggcagcctc ggtaaaggtg
    27901 accaccagca gttcttcaac gatcagcggg cggggaaagg cggcggaacc gcctagtcca
    27961 agtaacaggc gcaaatagag cgccgcaatc gtaaaggttt tgcctgtgcc ggcagaggct
    28021 tcaatcaggc gctcgccctg taagggcaag cgcaaaggat ctagtgtctc ggcgacatca
    28081 ctcattcttt tcactcatca ggggcattgt ttgctgcaac gcgctgacgt tctcccacac
    28141 tttccagcct tcaggatgca catattcggc tttcccgttc tggctgccgg aaatctgcga
    28201 cagaatagcc atgccttgcg gctcgaccac cgcctgatgg aagaaatcag caagtttttg
    28261 cggcgtcagc agttttatct gggccacgat tttatcacgc gaatcgaagc gcatattgcc
    28321 gcgatcgaaa tctttactta acttcgatgc ttcttcgccg agcgtttgcg gtgcctgcag
    28381 catctgggta attaccgcct gctggatttg cgcaaactca tctggcttca tcgctcgcaa
    28441 ttttgcctct gcggttggga aaaacgcctt gtaacgctcc cacaagaatg aaggctgttt
    28501 atcattgctt tgcaaaagga agcccatgcc ccactgacgc cccacgctca ttggaaacgc
    28561 aaacacggca tagcccaatt gttcttcggt acgcaactga ttgtagaacc acggctgtac
    28621 gatctgcccc aacagagagc tataggctga gctggtgtat tcatcgtagc cagtcggtac
    28681 aaataccgct gccagtgcgg agtcggtgct gttaccggct ttttcaaaga tgacggattg
    28741 ttttttatcg accactacat ctttgtttcg acaccactct gaaccatcag cgcccaactg
    28801 tttttgcaca tcgcgtgcca gcgttgttgc ctgggcctcg gtcatgttgc cgataaccat
    28861 aaactctggt cgagcccctg attttaaggc gtcgcgatag gccagcacct ctttcaacgt
    28921 aatggagggc aaaattttac gccgttcatc tcgcgagaag tacggcactt gcgagagcat
    28981 ctgcgcgggc ataatcgcct gctcaaacgc tttacccttt tctgcggaat ccatcatctg
    29041 gttataccag gacttcgcct gctcaagctg ctcttccgta gcggtatagc taaagtaccc
    29101 ctcgagcaat gcctggaaca gctgcggcag acgctgggtg taaccattag cattaatcat
    29161 aaggccgttg ttagcgttgg tggaaaaact tatgccacca accgacgcct ggttgcttaa
    29221 ctgatcaagc gccagccctg cgagataatc attgagcgca aacatcacct gattgcgggc
    29281 gctgtccatg gctttcggat tacgcaaaat caggctgaca tcagctttgg gctcgctggc
    29341 aaaataacgg cttggcgcat acaccacgcg cagattcgac tcatcaacaa tcagctctgg
    29401 atggtcgtat ttcttctctg acttaatcag cgagaaatca tcaggaatat aagggttaag
    29461 ctctggcaaa gagagcgcaa tgtcggcggc ttttttctgc cagtcggcga aagtttgtgc
    29521 gctgatttta tcgacctgat acggcgcatc gacaaagtaa gccgttttgt tgtgcggctc
    29581 tttcgggctg atataccaga tacgcgcatt ctgcggcgtc atcatcgcca gacgttcctt
    29641 tactgcttta gcatcgtacc gatcggcaat attgactgca tccagcgtat gctcaacagg
    29701 aacgcgaatc atggtatctg ccagccattc gacgtaatcc atatcacggg tgatcgacgg
    29761 ataacggaag tcgatatcca gcacattcgc cagttcatcg aagtattgtt tatcaatgcc
    29821 tttttcacgt aacagattga gatagctaaa aattgccgcc acaacctgat cgcgattagc
    29881 caggccttta tcagttaaag acgcagagat cgctaatacg ccgctgttgc cgttgacgat
    29941 aggatcggag ttggcgctaa tgccctcaac taatccctgc ttttgcagcc agtcagaaag
    30001 tgtacctggg ctgcgattgc caatcagata ggtaatcaat tcatcggttt tactacggaa
    30061 cttcgctgag ttgttatcga tgcgaaactc aacgcgcaac actttacgcg gcagcgcagg
    30121 gacgtaatga atgataatgc ccttttgcgc gtcggtgact accggcacgg tgatttccgg
    30181 ttttttgctc tctttgttcg gcacgcgacc aaaggtgtcc gccgccattt ttgccaactc
    30241 cggcagcggt ttattactgt aaataaccgc cttcatcaaa ttggcggaat agtacttctc
    30301 gtggaaatct ttcagcgcct gctgcaccgg attaccaggt ttgtcgctta aagtttcgag
    30361 gttaccacca gaaaactttg aaccggggtg tgccgggtta atggtttctg cgctgacctg
    30421 tgccatgcgc atcccgtcac gcgtacgcgc catggttaat tcagcgttca ctgcattacg
    30481 ctcacgttcg gcgtatttct tgtcgagcaa gggttctgca atagcatcgg ccaggcggtc
    30541 taccgcaccg ggcaaggcgt cgttctcaac ttccagatag aaagccgtgc gatacggcgc
    30601 agtgctggca ttgtgactac cgccgtgcat tttgagatat tcggccagac tgtcagcctg
    30661 cgggtacttt ttcgacccca tcagactcat atgttcaagg taatgtgcca gcccctggta
    30721 cgcctcggga tcttccagcg acccaacggg cactaccagc gccgagagcg atttaactgc
    30781 ctgcggatca gaaaccagca agaccaccat accgttatcc agacgtatag cctgatactg
    30841 gcggttatct ttatcacttt tacggatggt ttcctgaatc ggctgccatc ccgtttctgc
    30901 ctgacttaat ggtgcccaga gggcaacaaa caacaataat gctttgaacc aggtgctgcg
    30961 gggcattcac ggacctcata atcaacttta ttttctggac agattcaaca ggttaacgca
    31021 aatcatctgc cagaatttaa tcttgtgctg cacgagtcag cctatgttta tataaccatc
    31081 agtccgtgac tggtgcgcat cataaagtaa gcagatagat tgcgcaattt ttatacaaca
    31141 ctcatgactg attaaagcga aacagcggta acaggaaacg ttgcgactgt tcaacgatgg
    31201 cctccattgt ctctggtgtt aattgccgcc agagccgttg ataccagata tcatcacctt
    31261 cgccacgcac catcatgttg ccttcgtaag cctgaaggaa tttcgtacgg gctttttgca
    31321 acgtggaatc gtcatccagc atggcatcgt tttgcgcgtc ataacaggtt tttagccacg
    31381 cgccgccact ttcaggtaac accagcaatg gcgcggacat tccttcacga tacccctcaa
    31441 tcagttgtga gaggtaatgc aaagcctgtt cggctgcaag cggcggaaaa cgccactcac
    31501 cgtctttgcg tagaaaaagg cgactttcgc cattaccacc gctggcacag tagacaaggt
    31561 gttccagcca aagttgcatt ccctgcgcca cacttaataa agagggacgc cagcgcaaca
    31621 ggccatccgg ctgcacctgc ggcaaccagc cagttatctg cacaccgttg caggcgagat
    31681 caatttccat actctgcccc ggctggcgac aggcaatgac tctgtcggca agctgctgca
    31741 tctcctggca ctgtgtttcc cagaaaattt caccaaaagc gccatacggt aaatcccctg
    31801 ccgcccggaa gcggcggaac aagcgttcgg catcatcctg ctcaaccagt gcattcaata
    31861 actgctgatt gatttgataa cggctaagtc cttccagaat aaatggctcg gtgtctggga
    31921 tttcgctgtc ttcagtatgg aagttcacct gcaaacgcat ctggaagaac gcccgcaccg
    31981 gatgtgccca gaatcgttgt agcgtttcca gcggcacggt ttccggtaag gtaaacggca
    32041 gcggctgaac aaattcagaa tgtgctttac cagcctggct ggccgcaggt agccattcac
    32101 gagcatagct ttgtcgttcg cctggctggt agttttgcgg atcaaacggc atccgggtat
    32161 ggaggcaagt aagatgcgct tttacccttg cctcgctttc atcacagttg agcgcttcat
    32221 cgcccggtag ataatgactt tgcccgatgt agtcgatcag ttcctgcacc agtaccgacg
    32281 ggaaacgttc actgttatcc tgaatggaac gcccgatata gctgatatag agtttttgct
    32341 gcgcggaaat taacgcttcc aggaacaggt agcggtcgtc atcgcgacgg ctacggtcgc
    32401 cacgcttcgg tttctggctc atcaggtcaa agcccaatgg cgcaagctga cgtggataaa
    32461 cgccgtcgtt cattcccagc aggcaaacca ctttgaacgg aattgaacgc attggcatca
    32521 gagtacaaat gttaaccggt ccggcgagaa aacgctggct gatacgttct tgatccagac
    32581 gctgtgccag ttcatcacgc aatagtgaca gcggcaccgc gtcgccatac tgcgcaccta
    32641 aaccttcggc gataatcgcc tgccattgtt gttcgatcag cgtcatcgcc gcttcggttt
    32701 ccgcatccgg caggaagaag gcgttgagca tatcgcgaca aaccggcaac cactcttcta
    32761 gcggacgctc ctgtgccagc ccgcgacgcc agatgtttag ctgcattagc agtgaagcca
    32821 gatgccccac cagttctgca attaagccgc tcgattcatc ataaggtaga accgattgcc
    32881 actcgccctg cgcgctctcc atcgcgtagc ccagcaacat acgcgtcagg ccaaatcgcc
    32941 aggtgtgttg tccggtggcg gggagttcca gctcgcgaac gttgtcgtca tctatccccc
    33001 aacgaatgcc ggattcgttg acccactggc gtaaataacg cagcccttct tcggtgatgt
    33061 caaaccgcgc cgccagcacc ggcacatcca gcagcgccag cacatcctct gacacaaagc
    33121 gactgtcagg cagtgataac aggctgataa acgcttccag taccggatgt gactgccgcg
    33181 cacgacggtc ggaaatggcg taaggcaggt aacgatccgc aggtgcgcta ccaaacactg
    33241 cctgaataaa cggactgtag ctgtcgatat cagccaccat cacgatgatg tcgcgcggag
    33301 taagtgtcgg ggcttcctcc agcatcgcca gcaagcgatc gtgtaaaact tcaacttcac
    33361 gctgcgggct atggcaaacg tggaatgtga tactgctatc cagtggatca agcgggcgtt
    33421 tgttatcgct acgggaaaac tcttcgatgt tcacacccgc aacggcgcgg ttttccagtt
    33481 ccagaatgtc agactgaatg ttatgcagca ggttatctgg cgtcacatcg acaaaagcgt
    33541 ccagctcctg gctgctctcc aggtcagaaa ggagataaat gtagtcgcgc ccaagcttcc
    33601 cccatgaggc cagcagcgag ttgccgacat cctgttcacc atcgctgtta aagagctgtc
    33661 cggcattttc gctatcgcga aatagcggca attcgcgatc ttcaaaactg tgtcgacgct
    33721 gacgagtcag cagtttcgcc agataagcag gatctttaat atcgccccag taataacggc
    33781 aggggttggt aaacaggaga tggatttcaa tatgtttacc cagcgcctgg agcgcctgga
    33841 gataaacagg cggtaacgcg gaaataccgc atataaagac gcgcgaaggt aaccccggcg
    33901 ggcaggtcgt cgcggactcc agcgtttcga taaagcgctg atagagattc gcgcggtgcc
    33961 agcgcggttg tccgagttca tgggtatatt ccaccagcgc cttccacaac ggggcttgcc
    34021 aggcctgtgc ttctcccagc ccttcaacca aatgtcctgt ttcccactgt gccagccagt
    34081 ccggacgata gaccagatac tggtcaaaca ggtccgccgc ttttgaggaa agctggaaca
    34141 gttttcgctt gtcgctatcg tcagtcagat aatgccgcaa cagggtaaag tcttcgcgct
    34201 ccagcaattg cggcagcaga gtcatcagtt tccagctcat gctctgtttg ttaaaggcgc
    34261 tctctttggg gatttccggt aacacccgga cgaacatatc ccagataaag ctcgctggca
    34321 gcggaaaatc aatgtttgcc gcaataccaa acttttgcga cagggtcatt tgcagccact
    34381 gcgccatacc ggtactttgc accagaatca tctctggttc gaaaggatcg tccagccgtt
    34441 cgcgttcgac aataaactcc atcaacgctt ccagcacgtc cagacgattg gaatggtaga
    34501 cccttaacat agcggctcct gactactgac aattcgggca atgcaggcgg gtcatctcgc
    34561 cttctctgcc cccgggtgaa actagcgtaa cgctgatgct gacacatccc gcctgcgatg
    34621 tctgcattcg gttgacctgc cagttggcag gtggcgaaat cgcgcgcagt tgcgtttgct
    34681 gccaaccatg ccgccagagc ttttggtact ggtttctgct ggcgagactg ttcattgatg
    34741 ttcgctggat acccgataac gcagtgacaa tcatcaccat cagcaccatc agcaccatca
    34801 gcaccatcag caccatcgca accattacct ccggcaggct aaagccttgt tgattcttca
    34861 gggaagctga cataatgccc cctctttcag cggacaaaaa tcgctccagc cgcgtggcga
    34921 aaaaacaatt tttccatcaa tgacttcgcc tgttcgccac aacgaaacgc cttcataacc
    34981 ggcaatcaat aaggcttcat tatctgccag taaacgcaaa caaacctggg catcggttcc
    35041 agcgtactgc gagcactgaa ctgctggctg cgtctgccag gagtgcattt ttccccacgc
    35101 cagcgccgac tgaacgatgg cctggcggcg caatgactga ctttccatgc tcacgcgaga
    35161 ggcaaaactg cgatcctgct gactcattcc ttgcaatagc aagctaccca aaaccagcag
    35221 catcaggacc agagccagtg acgaaacacc tttttcgcgg ttcacaggtt gaatcctgtc
    35281 acgctatagc tggcatccac cacggtttgc ggttcagact tactggcagc acgcatatta
    35341 accgtcaaca ccggcgagaa gccgctgaca tcctgacgta cgacctgaaa agtgtcgata
    35401 atgatggcat ccggattagt gactttatcc cagcccttac cttcacagga tgtcgcaccg
    35461 cgtagcgttt ccagcacatg ctccttcaga cgaaatccaa tctggtcgga ctcttttacc
    35521 ggttcgcgat cccagatacc gttactgttc gcatcccact gcacaatgat acagtcaccc
    35581 tgtccgacaa tttccagccc ttcgccggta cagctgccat gacaataacc cgccctctgg
    35641 agatgcttcg cgacggtaaa tacccgcagc cagatttcat cttccagtgc cagcttacga
    35701 gtgctcgtta aactttcacg ctgtaacgca ggcagaaagc gtgcagcccc cagcaacaat
    35761 acgctactga tcgccatagc aatcaacact tccagcagag aaaaaccttg ctcttttaca
    35821 ggcatccttc tgtttctcct tgctgataaa gccggagtct tccccacggc gaaaccacca
    35881 gccaccactc gcccgttgag tttttgaagc gaatatgcac ggcccatgcg gtattgcgca
    35941 ggccaaagaa agcaagcgaa ggtgtcaggt cgctcatttc gacttcgggc cagcgtggca
    36001 caaagaccaa tggtgaactg ccatgacagg tattggcccc agcagcggaa ctcacaaggc
    36061 accataacgt cccctccctg ataacgctga tactgtggtc gcggttatgc cagttggcat
    36121 cttcacgtaa atagagcaaa tagtcccgcg cctagctggc ggtttgccaa agccgttgcg
    36181 actgctgcca gtattgccag ccatagagtc cacttgcgct tagcatgacc aaaatcagca
    36241 tcgcgaccag cgtttcaatc agcgtataac cacgttgtgt tttcatgccg gcagtatgga
    36301 gcgaggagaa aaaaagacga gggccagttt ctatttcttc ggcgcatctt ccggactatt
    36361 tacgccgttg caggacgttg caaaatttcg ggaaggcgtc tcgaagaatt taacggaggg
    36421 taaaaaaacc gacgcacact ggcgtcggct ctggcaggat gtttcgtaat tagatagcca
    36481 ccggcgcttt aacgcccgga tgtggatcgt agccttcaat ctcaaagtct tcgaaacggt
    36541 agtcgaagat ggattcgggt ttacgtttga taatcaactt cggcagcgga cgcggttcgc
    36601 ggcttaattg cagatgagtt tgatccatat ggttgctgta caggtgcgta tcgccaccgg
    36661 tccagacaaa atcacccact tccagatcgc actgctgcgc catcatatgc accagtaacg
    36721 cgtagctggc aatgttgaac ggcaggccga ggaagacgtc acaggagcgc tgataaagct
    36781 ggcaagagag tttgccgtct gccacataga actggaagaa tgcatggcac ggtgccagcg
    36841 ccattttatc cagttcgcct acgttccacg ctgaaacaat aatgcggcgc gaatccgggt
    36901 cgtttttcag ctggttcagt accgtagtaa tctggtcaat atgacgacca tctggcgttg
    36961 gccaggcgcg ccactgttta ccatacactg gcccgaggtc gccattttca tcggcccatt
    37021 cgtcccagat ggtgacattg ttttcgtgta gataagcaat gttagtatcg ccctgcagga
    37081 accacagcag ttcgtggatg atggaacgca ggtggcaacg tttggttgtc accagcggga
    37141 atccatcctg caggttaaaa cgcatctgat gaccaaaaat ggaaagcgtt ccggttccgg
    37201 tacggtcgtt tttctgtgtg ccttcgtcga gcactttttg catcagttct aaatactgtt
    37261 tcatggttcc tcaggaaacg tgttgctgtg ggctgcgacg atatgcccag accatcatga
    37321 tcacacccgc gacaatcatc gggatggaaa gaatttgccc catgctgatg tactgcaccc
    37381 aggcaccggt aaactgcgcg tcgggctggc ggaaaaactc aacaatgatg cgaaacgcgc
    37441 cgtagccaat caggaacaag cctgagacag ctcccattgg gcgcggttta cgaatataca
    37501 ggttgaggat aataaacagc accacacctt ccagcagcag ttcgtaaagc tgtgatgggt
    37561 ggcgcggcag cacaccgtaa gtgtcgaaaa tggattgcca ctgcgggttg gtttgcagca
    37621 gcaaaatatc ttctgtacgg gagcctggga acagcatggc aaacgggaag ttcgggtcaa
    37681 cgcggcccca caattcaccg ttaataaagt tgcccagacg cccggcacca agaccaaacg
    37741 gaatgagtgg tgcgataaaa tcagagacct ggaagaagga acgtttagta cggcgggcga
    37801 agataatcat caccacgata acgccaatca ggccgccgtg gaaagacatg ccgccgtccc
    37861 agacacggaa cagatacagc ggatcggcca taaactgcgg gaaattgtag aacagaacat
    37921 aaccaatacg tcccccgagg aagacgccga ggaagcccgc atagagtaag ttttcaactt
    37981 catttttggt ccagccactg cccggacgat tcgcccgacg tgttgccagc cacattgcaa
    38041 aaatgaaccc caccagatac atcaggccgt accagtgaag cgccacgggt cctattgaga
    38101 aaatgaccgg atcaaactcc ggaaaatgca gatagctact ggtcatctgt caccacaagt
    38161 tcttgttatt tcgctgaaag agaacagcga ttgaaatgcg cgccgcaggt ttcaggcgct
    38221 ccaaaggtgc gaataatagc acaaggggac ctggctggtt gccggatacc gttaaaagat
    38281 atgtatatga tccgcgctat aaccctccgc gaatcagccc gcccatgccg cgacgctcca
    38341 taaaggctgc aacctgatga cgaacttcgg tcgccagttg cgcttccaga ctacgctgcg
    38401 caagattttc tgcttcggca aaatcaatgc gccgcagcag gtattttacc cgcgctacag
    38461 aacgtccgtt catagacaga tggcgatacc caagcccaat gaggattgcc acgcacatgg
    38521 gatcgcccgc catttcaccg cacaaacgaa gatcgatacc atgtatttcc gcttcccggg
    38581 cgatcatcgc cagagctcgt aacattgcag gatgaagact gtcgtaaatg ttcgccaccc
    38641 gggtattgtt gcgatcaacg gccagaatgt attgagtcag atcgttggtg ccaacagaga
    38701 tgaaatcgac cctctttgcc agatgcggca gcataaatac cattgacggc acttccagca
    38761 tgatgccgat acgtggtttg ggaatttcgt aaccgatcat ctcctcgact tcacgtccgg
    38821 cacgttcaat caggcggcgt gcttcgtcaa cttcatcgag gctggtgacc atcggcaaca
    38881 gaatattcag gttgcccgta gcggcattag cacgcagcat cgcccgcacc tggatcaaga
    38941 agatctccgg ctgatcgagc gtaatgcgaa tcccacgcca acccaggcat ggattctctt
    39001 cgctgatcgg catgtaaggc agctgcttat ctgctccgac atccagcgta cgcaaggtga
    39061 cgggtttatc attgaacatt tgcagcatcc cctgatactg cgccacctgt tcttcttccg
    39121 acgggaaacc actttgcagc atgaatggga tttcagtgcg ataaagaccg atgccatcaa
    39181 tacagctgcc cagtttttct tcatgttccg ggcttaaacc agcattgagc atgactttta
    39241 tacgctcacc gctttttaac tgcgcgggta aattgacgtc atcttccgcc agacggctaa
    39301 gctcaatctc ttcactaatt agccgctgat attcttgcag cagtaccggc tccggatcga
    39361 ccagcaattc accgcgatag ccatcaacga tcagcgtcct acgatgcagc accgaaggct
    39421 gaatatccgc gcccatcacg gtagggatcc ccagcgcacg taccatgatc gcagcatggg
    39481 agttggctgc gccatctcgc acgacaacac cgactaagcg atcctggggc agctcagcaa
    39541 gagttgtcgc tgacagttca tctgccacca gaatgaaacg ttccggccag gcgttcggcc
    39601 cttgattagc gtcatcaaga tgaaacagca atcgctgacc cagcgcacgt aaatcaccag
    39661 cccgctcttt gagatagtta tcgcttagcg cggcaaactg ttcggcaaat ttttcaatga
    39721 ccgtttttac cgcccactct gccaccgagc ctttatcaac ctcggcaaac aattcgcgac
    39781 gcagccgggt gtccgaaagc aggtgcgagt aaagatcgaa aatagccgcc gtttcttttt
    39841 gtgctccggc ggcaaagcgt ttgctgtagc ggcgaaactc gtttgccgct tcttccagcg
    39901 ccccggtcag tcgttcgcgt tccagagccg gatccagcgt tgatgcctga tacacctgtt
    39961 ccattaaagg taacgtggca tcctgccagc cttcggcaat cgccacacca ggtgctgccg
    40021 gtaatgcgcg gattcgcgtc tggcgatatt gcccaaacag ggcagtcaac tgcgactgag
    40081 aaagaatagc tgccatctgg gtggcaagcg tcaccaggaa ggattcttca ctttcgtcat
    40141 actggcgcaa ctctcgttgc tgtaccacca gtacaccaag caactggcga cgttgaataa
    40201 ttggtacgcc taaaaacgcg cggaaacgtt cttcttttac ggaggggatg tatttgaagc
    40261 tggggtgctt ttgcgcatct gcaaggttta tcggttccgc cagcctgcca accaggccga
    40321 cgatcccttc atcaaacgcg agcgttacag tgcgaccgcg tggttttttc agcccccggg
    40381 tcgccatcag gtagtaacaa cgtcgatcat gatcggccag gtagaccgaa cagacctcgg
    40441 tatccatcgc aagacagatg tcggtaacca gaatatttaa cgcctcattc aggcgtggtg
    40501 cgctggctac cttttcgact atttcgcgca ggcgagtgag cataattggc gtgacttaac
    40561 ctcttttacg tcgataagca gatgcgtttt gtggttttgg cgtattttcc tgcagtgaca
    40621 tcaccacact cgcgaactct ttcattaccc tacggtagac atcacgttta aatgacacca
    40681 cctgtctgac cggataccag taacttaccc atcgccagcc gtcaaactct ggtgtactgc
    40741 tggtttgcat attgatttct gcatcgccgc ttaccagctg caagagaaac catttttgtt
    40801 tttggccgat acaaaccggc ttcgtgtccc aacgcaccaa acgtttcggt aatttgtagc
    40861 gcaaccagtt acgcgttgaa gcaaggattc gaacgtcttt gcggcttaat cctacttctt
    40921 caaacaattc acggtacatc gcctgctctg cggattctcc ggggttgatt ccgccttgcg
    40981 gaaattgcca ggagtgctga ccaaatcgcc gggcccacat tacctgcccc tggcgattac
    41041 aaatcacgat acctacgttt gggcggtagc catcgtcatc aatcaccgga ctacctcaaa
    41101 ataaagcttt atatacgaat gattgtttca tactccagga agacggtaaa ccactctctg
    41161 cagggcatta cacactaata acaattgaat aactcacagt tatgtgcaga gttataaaca
    41221 gaggaagggg tggatagccg tttttattca ccttttctgt ggatagagtt gtgaagaact
    41281 acggaattac tacgggaaaa cccggagcat tctgaataag cccgatatag acattttaaa
    41341 atattcatta ttagtcaatg cattacatgt aatttgagta atgaaaaaga gtaattcgtg
    41401 acccaggtca cacctctcat ttacgggttg gcgaaagatc aaccaatgcc gtgtttatcc
    41461 acagaatgtg ccactaagtt aagcactgaa ccactaaaaa ctggagtttc gtcgcacgtc
    41521 aaggctgtaa atgaaaacag tagtggaggt ttttcacagt tatcccagct ttctgtggat
    41581 aacatggtgt aagatcctgt ttattttcag tgaccagatt tggaaaacct attgcagtgt
    41641 tgcgcaactc gattaccggc agcctaaaaa gcaatataaa tcagagaatt gaacaacaca
    41701 tgtggaaaaa gttacactgc gaatattcgg cacataattg ctgtttgttt tttaaccaag
    41761 gtatcatgac atgtcccaac ctcgcccact gctctctcct cccgaaactg aagaacagtt
    41821 gttagcgcaa gcacagcaac tttctggtta tacattggga gaactggcgg cacttgacgg
    41881 gctggttacg ccagagaatt taaaacgcga taagggctgg attggcgtgt tactggagat
    41941 ctggctaggt gccagcgcag ggagtaaacc tgagcaagat tttgctgctc tgggcgtgga
    42001 acttaaaact atccctgtgg atagtcttgg tcgtccgctg gaaacaacat tcgtttgtgt
    42061 tgcccggtta acgggtaata gcggggtgac ctgggaaacc agccatgtgc gccacaagct
    42121 caaacgcgta ctgtggatac cggttgaagg cgagcgcagc atcccgctgg cgcagcgtcg
    42181 cgtaggatca ccgttgctgt ggagcccgaa tgaagaggaa gaccgacagc tacgcgaaga
    42241 ctgggaagaa ttaatggata tgattgttct cggtcaggtt gagcggatca ccgctcgtca
    42301 cggggaatat ttacagataa gaccgaaagc agcgaatgcg aaagcgctta ccgaagccat
    42361 tggtgcccgg ggcgaacgga ttctgacgct gccacgcggc ttttatttga agaagaattt
    42421 caccagtgca ctactggccc gtcattttct gatccagtag ccatcgcttt gacctgccgc
    42481 tttccgggca tataattacc gcttcatttt tttggcaggg ctttttagat gttatttgca
    42541 tggataaccg atcctaacgc ttggcttgcg ctcggtacgc tgacgctgct ggagatcgtt
    42601 cttgggatcg acaatattat tttcctttct ctggtggtgg caaagcttcc cacagcacaa
    42661 cgtgctcatg cgcgccgtct ggggttggcg ggagccatgg ttatgcgtct ggcgctgctg
    42721 gcatcaatct cttgggttac gcgcctgaca aatccgcttt ttacaatatt cagtcaggaa
    42781 atttctgccc gtgatttgat tctgcttctg ggtggcttgt tccttatctg gaaagccagc
    42841 aaggaaatcc acgaatccat tgaaggtgaa gaagaagggc tgaaaacacg cgtttcatca
    42901 ttcctcggcg ctatcgtgca gattatgctg ctggatatta tcttcagcct cgactcggtg
    42961 attaccgctg tgggtctgtc agatcacctg tttattatga tggcagccgt ggtgattgcc
    43021 gtaggcgtga tgatgttcgc cgcgcgctcg attggtgatt ttgtcgaacg ccatccttcg
    43081 gtaaaaatgc tggcgctctc tttcctgatt ctggtgggct ttaccctgat tctggaaagt
    43141 ttcgacatcc acgtaccgaa aggttacatc tacttcgcga tgttcttctc tattgcggtt
    43201 gaaagcctca acttgattcg caacaaaaag aatccgctct gatactccgt acgctctcct
    43261 gcgggagggc gtactcactc cttttaccct cacagtttaa gattttcctg ctttcaagat
    43321 atataacgtc ggtttataaa cagactatta tcataggtag gccaaatgag gataaccgga
    43381 tgaaaaaatg ggcagtaata atttctgcag tcggactggc gtttgctgtt tccgggtgtt
    43441 ccagtgatta cgtcatggcg accaaagatg gccgtatgat tttgaccgat ggaaaacctg
    43501 aaattgatga tgataccggg ctggtgagtt atcacgatca gcaaggtaac gcgatgcaaa
    43561 ttaaccgtga tgatgtttcg caaattattg aacgttaaca aataaggtca gcatccggct
    43621 ggccttaaga tttttctctt tcccttttcc cttccctctg ccatttttat attccttatg
    43681 tcgtgattat aaaaaggaaa cggctatgca atatcaccgt ataccccaca gttcgctgga
    43741 agtcagcacg ctggggcttg gcacgatgac gtttggtgaa cagaacagcg aagccgacgc
    43801 ccacgcacaa ctcgactatg ccgtcgctca gggcattaac cttatcgacg ttgccgaaat
    43861 gtacccagta cctccgcgcc ccgaaacgca agggttaacc gaaacctacg tcggcaactg
    43921 gctggcgaaa catggcagcc gcgaaaagtt aattatcgcc tccaaagtga gcggaccgtc
    43981 gcgcaataat gacaagggca tccgcccgga tcaggcgctg gatcggaaga atatccgcga
    44041 agcgctgcat gacagcctca agcgcctaca gactgattac ctcgatcttt atcaggtgca
    44101 ctggccgcag cgcccgacca actgcttcgg caaactcggt tatagctgga cggattctgc
    44161 gcctgcggtt tcgctgctgg atacgctgga cgcactggca gagtaccaac gcgcgggaaa
    44221 aattcgttat atcggcgtgt cgaacgaaac tgcatttggc gtaatgcgct acctgcatct
    44281 ggcggacaaa cacgatctgc cgcgtattgt caccattcag aacccttaca gtctgttaaa
    44341 ccgcagtttt gaagtaggtc tggcagaagt cagccagtat gaaggggtcg aactgctggc
    44401 ctattcgtgc ctgggtttcg gcacgctgac cgggaaatat ctcaatggtg caaaacccgc
    44461 tggcgcacgt aatacgctct ttagtcggtt cacccgctat agcggtgagc aaacgcaaaa
    44521 agccgtcgcg gcgtatgttg atatcgccag acgtcatggc cttgatcctg ctcagatggc
    44581 gctcgcgttt gtacgccgtc aaccgtttgt tgccagcact ctgctgggcg caaccacgat
    44641 ggatcagctg aaaactaaca tcgaaagttt gcatctggag ttaagcgaag acgtattagc
    44701 tgaaattgaa gcggtacatc aggtttatac ttatcctgca ccataaagat taacgccggt
    44761 taaaaccggc gttaaatatt aatgacggcg ctgccagatc cacagcgccg ttattgccag
    44821 cgcaaatagc gcaccgaagc caatgccaat gggcacgacc gggatgccta ccattaccgc
    44881 cagcgagtaa atgcccagca tcaacaacat ggcgctgttt tcgccaaggt tttgtactgc
    44941 aatcgcattc cccgccccga cgcttttttt accccgctcc tgtagcaacg cattgagcgg
    45001 aacgacaaaa aagcccccca gcacgccaat cagcatcaac aaggcatagg ctggcagcag
    45061 ctcgtgttgc agggagaaaa tcggtaccac cacgccaatc aaaatcccgg ctggcataca
    45121 gcgtgacacg gtttccagcg taaccagctt cgctgccgca cctgcgccaa ccacgatacc
    45181 aatcgctacc atcgcgttga gataggtcgg cgtagcgtta tcggtaatgc ccagcgccac
    45241 cggtacccac agcaccaaca ggaaacgcag cgtgacaccc gctccccaga ataaactggt
    45301 gcccaccagc gaaaaacgcg tttcaccatt gcaccatagc gaggtgcagg cattcaggaa
    45361 actgcgggtc atgttgatga gattccagga ctgccccgga cgcgccgcca gtttgggaat
    45421 gtagatattg gcaacgaccg caccaccgta ggccagtgcg catgcggcca gagcgaccag
    45481 gacatgccag tcagccagca caccaccggc tacggaaccg agtaaaatcg ccgctatggc
    45541 agaagcttcc attaaaccgt tagcttttac taacttacta cccgtggtta attcgccgag
    45601 aataccgtat ttcgccggtg aataggctgc agcaccaaca cccaccagcg tatagccgag
    45661 aaacggattg ataccaaagc agatactggc tgcgcccagc agcttcaggc cgttggcaaa
    45721 catcatcacc cggcctttgg cgaagctatc cgccacctgc ccgacaaacg gcgccaaaag
    45781 aatgtaagca cctacaaaca ccatttgcag gatgggctgg ctccactccg gatagaactg
    45841 cgctttcagt aacgccagag tggcaaacag tagcgcgtta tcgccaaacg cagagagaaa
    45901 ctgcgccacg ataaccgctt tcatcccctt cgaccacaac gaagtgttag tgtgcactga
    45961 ctcactcatc gtgttgttcc gcttcgtcta cccagctttt caacgtgaca aagtcaggtt
    46021 tgccgctgcc aagtaatggc atctgtttca gatagcgaat atcgcgcggt acagcaagct
    46081 ccggcacgcc gtgctcgcgg gcatactgtt gcaacttatc gcgcgtcagt tcgttatctg
    46141 tggtgaaaag caccagtgcc tcgcctttgc tggcatcgct cttaatcgca gtggcatgga
    46201 ctttatctgg cgaaacacca agtgccagtt gttccaccat ttccagcgac accatttcgc
    46261 ctgcaatttt ggcaaagcgt tttgcgcggc cctgaatctg cacaaagccc tgctcgtcaa
    46321 aacgcacaat atcgccagtg tcataccagc cgcgctccat ttcgccgcga acattctcgg
    46381 cggtgggcac ttccagtaca cctggcttct ccacccgcag atagccgttc attatgttcg
    46441 gccctttcag ttgcaggcgt ccgccctctt cgataccagg gaccgacaac aggcgtgcat
    46501 ccattcctgg tagaatacgc cctaccgtac cgggtttcgc cgccatcggt acgttgatag
    46561 aaacgacagg cgcgcattcg gtcacaccgt agccttcaag gatgcgcagg ccaaatttat
    46621 cctgccaaag ctgtttggta ctttcttgta gtttttctgc gcctgccacc acatagcgta
    46681 gacgatagaa gtcatacggg ttggcgaaac gcgcgtagtg accgaggaaa gtcgaggtac
    46741 cgaacaacac ggtgcaactg cggtcataca ccaagtccgg cacaatgcgg taatgcagcg
    46801 ggcttggata aaggaacact tctgcacctg taagcagtgg cgtaaacagg cctaccgtca
    46861 gcccaaagga gtgaaacagc ggtaatgccg acataaagcg atcgttggtg gtgaagtcgg
    46921 caatcgtttt aatctgctcg acgttcgcca gaatgctttt atggctatgg acgacgcctt
    46981 ttggatggcc ttcagaaccg gaggtaaaga ggatcagcgc ctcttcttcc ggctgctgtt
    47041 ttacctgcgc caggcgcggc atcagcaaat gagcgaagat ccatactttg tcggcagtgg
    47101 tgacatctgc ttttaaatct tccagataga cccagcgcac ctgagtaagt tgctccggca
    47161 gatgccagag tttgccttta tcgagaaact ggcgggaagt gaagatggtt ttgatttcag
    47221 ccgccgtaat agcactggtc agccctttta ccccggcagt gtagttcatc attgcgggaa
    47281 tgcggcgacg cgcgatggcc ccaaaaatca ctgccgcact gatgcctgca ttgggcagca
    47341 ttaagccgat gcgttcgcct tcaacactgt atttttcaag gatgcgtcca acaaacagcg
    47401 ttttcgtaag caatttgcga taggagtctg gggtaaagtt gacgtcttcg acacatttct
    47461 tcccggctcc gaagcggtac attgcactca gtaaagattc gtacagagtt tcacgcgggc
    47521 gcaccgccat acgcgcttcc atcattattt gatgcagcat ttcgccagcg attttgcgac
    47581 ggtcacgggc acgcggcgca tccggcatcg ccacctgcgt tggaggcaaa atatgcagag
    47641 taatttgcgg gaacaagcga cgtttaacca gacctttcag gcggctgaag tgcgtaagtt
    47701 ccgccccttc aatacgcaca ggaataaccg ttgcaccaga cttcgccgcg acaaaacccg
    47761 cgccatcgta gattttcatc agcgagcctg tagtggtgat gcgtccttca gggaaaatca
    47821 ccactggtcg gccctgttcc accagacgta ccagatgttt aatagccata ggttgcgtcg
    47881 ggtcgagagg aacaaagtcg ataaatgatt tcagccaacg catataccac tgttggctta
    47941 ttgaggtgta aacggcaaac actggacgca caggtaaaaa cagtccaagc aaaatgccat
    48001 caataaaaga gacgtgatta ggcgtaatta gtacgcgctc gcccttcagt gcctgggtgt
    48061 cacccgtaac gcgaacgcga tacaaaacac ggcacaaatt tcgaaaaaag ctaaaaagca
    48121 ttccaactcc ctttgctctg attcagtaaa agcgaatgga gggagattac acgagataaa
    48181 gaacgcgagc gacagtaaat taggtgcgaa aaaaaacctg cgcatccgcg caggttggtg
    48241 caagagacag ggtacgaaga gcgtaccgaa taatctcacc aatcaatacc tctgggatct
    48301 tgattgtggt ctgcacgacg actcttcgcc agcgagaaaa cgcaaaggaa tgaagggaaa
    48361 tgcaacgagg tgtgtaaatt gtcggttact gttacagatt gatgaccggc aaaaaaaaac
    48421 ctgcgcatct gcgcaggctg gtgtaattca tgtgctcaac acgaagttga cttcacctat
    48481 caatacctct gggatcacca ctttagcaac ctgaagccaa acgccaccag cggtcaatcg
    48541 caacagcgtt tcgcaaagtg taaccaaagg tttgcaatct ctttttttgt cttacttatt
    48601 tgcgtttggc tcacattccc acgatgaaaa cacgccaccc cttgaaccaa cgggcgtttt
    48661 ccgtaacact gaaacaatgt aagcgtttac ccactaaggt attttcatgg cgaccataaa
    48721 ggatgtagcc cgactggcag gcgtttcagt cgccaccgtt tcccgcgtca ttaataattc
    48781 acccaaagcc agcgaagctt cccggctggc tgtgcatagt gcaatggagt ctcttagcta
    48841 tcacccgaac gccaacgccc gtgcgctggc gcagcagacc actgaaacgg tcggtctgat
    48901 cgttggtgat gtttccgatc cgtttttcgg tgcaatggtg aaagcggtcg aacaggtggc
    48961 ttatcacacc ggtaatttct tattgattgg caacggttac cacaacgaac aaaaagagcg
    49021 tcaggctatt gagcaactga tccgccatcg ctgtgctgcg ttggtcgtcc atgccaaaat
    49081 gatcccagat gccgatttag cctcattaat gaaacaaatg cccggtatgg tgctgatcaa
    49141 ccgtatcctg cctggctttg aaaaccgttg tattgctctt gacgatcgtt acggtgcctg
    49201 gctggcaacg cgtcatttaa ttcagcaagg tcatacccgc attggttatc tgtgctctaa
    49261 ccactctatt tctgacgccg aagatcgtct gcaagggtat tacgatgccc ttgctgaaag
    49321 tggtattccg gccaatgatc ggctggtgac atttggcgaa ccagacgaaa gcggcggcga
    49381 acaggcaatg accgagcttt tgggacgagg aagaaatttc actgcggtag cctgttataa
    49441 cgattcaatg gcggcgggcg cgatgggcgt tctcaatgat aatggtattg atgtaccggg
    49501 tgagatttcg ttaattggct ttgatgatgt gctggtgtca cgctatgtgc gtccgcgcct
    49561 gaccaccgtg cgttacccaa tcgtaacgat ggcgacccag gctgccgaac tggctttggc
    49621 actggcggat aatcgccctc tcccggaaat tactaatgta tttagtccga cgctggtacg
    49681 tcgtcattca gtgtcaactc cgtcgctgga ggcaagtcat catgcaacca gcgactaacc
    49741 gcagttaaag caattccagc gccagtaatt cttcgatggt ctggcgacgg cgaatcaacc
    49801 gcgcctgacc attatcaaac agaacttctg gtaacagcgg acggctattg tagttggatg
    49861 acattgatgc gccatatgcc cctgtatcat gcagtaccag ataatcacct gccttcactt
    49921 ccggcaaggc gcgggtttca acatttcccc cttcctgctg ggtaaagaca tcgcccgatt
    49981 cacataacgg cccggcgacg acggtttcca ccgttggcgc gtgttccaga gaacgaccat
    50041 cagctgccag ggcactgata tggtggtaac taccgtacat tgccgggcgc atcagatcgt
    50101 tgaacccggc atcaaccagc acaaagtggc ggctacccat ttgtttgacg ctccgcacct
    50161 gagtaattaa tacgccagac tgcgctacca ggaagcgacc cggttcaatt tccagtttca
    50221 cagggtggcc caaatggcga gcgatttgct cacgcgcggc attccacaga ccataataat
    50281 gttcggtatc aaccgcctct tcaccctgtt gataaggaat agaaagccca ccgcccgcag
    50341 aaatagcctg taaatcctga ccgaattcga taacctgacg caccatagca ccacacacct
    50401 gctccagatg ggcataatca acgccagaac caatgtgcat gtgaatgccg actagctgca
    50461 gatgatgacg ttgtatcacg tccagtgcgg cgggcagatc ggtgtaccag ataccgtgct
    50521 tgctgttttc gccaccggta ttggtttttt ggctatgtcc gtgaccaaac cccggattaa
    50581 cgcgcagcca tacccgatgc cctggcgaaa cctggcccag ttggtcgagc gtatcaacag
    50641 aacccgcatt caccggaatt tgcaattcac tgacgcgttc aagcgtcgcc tgatcgataa
    50701 catctgccgt aaaaacaata tcatcggggt gcgtttgcgg attgtaaccc gccgccaacg
    50761 cacgctctat ttcgcctaac gagacggaat ccactttcac gccctgctca cgcattaagc
    50821 gcaaaatatg aatattggaa caggctttct gtgcaaagcg caccacttca aactgtttca
    50881 gcgctgcaat ctgccgacga ataatttgcg catcgtagac ccacaccggg cagccaaatt
    50941 cagcgggcaa acgcagcaga ttttcggcgg tgagatcggt atcggtgctg aacagtgaat
    51001 gtggcataac aaactccaga tgagtgcttt tttatgatta cgccacatca taaaaagaat
    51061 aaaaaatatc gatttatgtc gagtctatgc aaaaatgata tggattaccg gattgcgaga
    51121 gagcgctaat ggccgccgtt aacttacgtc atattgaaat ttttcatgcg gtaatgaccg
    51181 ccggaagcct gactgaggca gcacacctgc tacacacctc acagccaacc gtcagccgcg
    51241 aactggcgcg ctttgagaag gtgatcgggc tgaaattgtt tgagcgcgta cgtggacgat
    51301 tacatcctac cgtgcaagga ctgcgtctgt ttgaagaagt gcaacgatcc tggtacggac
    51361 tggatcgcat tgtcagcgcc gcagaaagtc tgcgcgagtt tcgccaggga gaactgtcta
    51421 ttgcctgcct gccggtcttt tcgcaatctt ttttaccgca gctcctgcaa ccctttctgg
    51481 cacgttatcc cgatgtcagc ttaaatatcg tgccccagga atcaccgcta cttgaagagt
    51541 ggctctcggc ccagcgtcat gatttaggac tcactgagac actccatacg cctacgggaa
    51601 cagaacgtac cgaattactc tctttagatg aagtgtgtgt gttacctccg ggtcatccgc
    51661 tggcggtaaa aaaggtatta acgccggatg attttcaggg tgagaattac atcagccttt
    51721 cccgtactga cagctatcgc cagttgctgg atcagctatt tactgaacat caggttaaac
    51781 gacgcatgat cgtagaaacc cacagcgccg cgtcagtctg cgcaatggta cgggcggggg
    51841 taggtatttc ggtggttaac ccgctcaccg cactagatta tgcggcaagc ggtttagtgg
    51901 tgcggcggtt cagtattgcg gttccgttca ccgtcagcct gatccgcccc ctgcaccgcc
    51961 cgtcatcagc gctggttcag gcgtttagtg ggcatttaca agcggggtta ccgaaactgg
    52021 tcacttctct tgacgctatt ttgtcgtcag ctacgacagc ataaaagcga cagcatcctc
    52081 ggcatggatc gccgcggtat caaacacagg caaaacactg cgctcttctg gcaccagtaa
    52141 accaatttct gtgcagccaa aaatgacgcc ctgtgcgccc tgttctgcaa ggcgagcaat
    52201 cacttgcgca taataagcgc gtgacgcttc ggtaaattgc cccagacaca gttcttcaaa
    52261 aataatctga ttaattttcg cccgttcatc cgcttcagga ataagacaat tgatggaaaa
    52321 ttgttccgtc agccgcccgc gataaaaatc ctgttccatg gtgtaacgcg tacccagcag
    52381 cgccacacga gtcattccgg ccccggtaat tgcacgtccg gtggcatccg caatgtgtaa
    52441 gaaaggcaga gtgcaacgtg actcaatggc atccgccact ttatgcatcg tattggtaca
    52501 taacacaata ccttctgcgc ccgcccgctg taagccaagc gccgcctcag ccagaatgtc
    52561 cccggtttta tcccattccc cgcgacgctg gcactcttct atttcatgaa aatcgacgct
    52621 atgtagcagc atttgcgcag agtgaagccc accaagccgc tgtttaatgc cttcatttat
    52681 cagacgatag taaggaatgg tggattccca gctcattcct cccagcaaac caattgtttt
    52741 cattcctctc tcctgatgtg tgttaccgca acaaggctga ataaggaacg agacaaacgc
    52801 ctcaacggcc aggtgcccaa tctctattaa cgaaaaaagg gccggatgta cagcacatcc
    52861 ggcccgtgaa atcagacgcc gatatttctc aacttctcgc ctgccatcag tttgcgttcg
    52921 atatgttcca gcgtgacatt tttggtttcc ggaatgagcc agaaagtaat acccacaaac
    52981 gcaatgttca gcgcagtgta gagccagaac gtaccggcag cgccaatgct atcaagcagt
    53041 gtcaggaagg ccgcgccgat aatcatattc gacacccagt ttgtcgtcgt cgaacaggta
    53101 ataccgaaat cgcggcattt cagcggctga atttcagagc acaggatcca caccactggc
    53161 gcggcgctca tcgcataacc ggcaatacac atcatcgtca tgccaacaga gagccaggac
    53221 aagccactgg aagccgtacc gttatcaaac tgcatcaggc aatagcccag caccagagtg
    53281 cctaacgcca tcacgctgaa accaattttc agagccggtt tacgacctgc tttatctacc
    53341 gtaaagaccg caatgaaggt cgcgaacata aaggtcagtc ccacgaccag agtcgcaatc
    53401 atctgttgtt ctgtggtcgt aaagcccgcc attttgaaga tacgcggcgc gtagtacatg
    53461 atgatgttca taccggtaaa ctgctgcatc gcctgcaaca acataccgag aaacacagca
    53521 cgacggacgt tacggttgat cttaaacagt gcccaaccgc cctgttttaa cttcaggctt
    53581 tcacgaattt cgttgagttc ttctcgcgct ttttccgacg tatcgcgcag catacgcaac
    53641 acttcttccg cctcaatatg acgccccttt tccgccagcc agcgcgggct atttggcagg
    53701 aagaccacca gaataatcag cagaactgct ggtaaagcaa gaacccccaa cattgcgcgc
    53761 cagttaccgc tataactgaa cgctgtatcg gataaaaacg ccagcacgat gccgagtgtg
    53821 accatcaact ggtacatact gatcatctta ccgcgaacgt tttcacttgc catttcagaa
    53881 agatacagag gagcggtgta agacgcgatc ccgacagcaa tgcccagcac cacacgagcg
    53941 gcgattaaca tctctacgct ggtcgcaaaa gcggacccta tagaaccgag tacaaacagg
    54001 atggcccccg ccatcaggct gtatttacgc cccaggcgga acgacagcca accattaaac
    54061 agcgcaccaa ttgctgcgcc gagcatcatg ctactaacca cccattcctg caaacgactg
    54121 gtcagcacaa agtgatcggt aatgaacggc aacgctccgg cgattacgcc gatatcaaga
    54181 ccaaataaca atcctgcgac cgcagcagct accgaaacaa acatattcat acgccgcgta
    54241 tcacgcaaag aacgtggcgt taaagcagat tccgtattga tagtaaccat tttttcctgc
    54301 cagcagagag taagacattg tgaaaaaata cgtgaacaat tcacacaggt gtcaggtcgg
    54361 aaacagcata aatatggatt aaattgctgc gacatgccgt tatgtgatgg atattccaat
    54421 tttaaaatta agttgaatta ttgagattat tattaacctc ctaatt
//
LOCUS       DAZPCS010000012        54465 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZPCS010000012 DAZPCS010000000
VERSION     DAZPCS010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 54465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54465
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.012"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            82..714
                     /gene="comR"
                     /locus_tag="ACHUQ7_000653"
     CDS             82..714
                     /gene="comR"
                     /locus_tag="ACHUQ7_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFR1134200.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(776..1315)
                     /locus_tag="ACHUQ7_000654"
     CDS             complement(776..1315)
                     /locus_tag="ACHUQ7_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFR1134201.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1542..2846)
                     /gene="ndh"
                     /locus_tag="ACHUQ7_000655"
     CDS             complement(1542..2846)
                     /gene="ndh"
                     /locus_tag="ACHUQ7_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFR1134202.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAIKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(3254..3796)
                     /gene="ycfP"
                     /locus_tag="ACHUQ7_000656"
     CDS             complement(3254..3796)
                     /gene="ycfP"
                     /locus_tag="ACHUQ7_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFR1134203.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(3819..4844)
                     /gene="nagZ"
                     /locus_tag="ACHUQ7_000657"
     CDS             complement(3819..4844)
                     /gene="nagZ"
                     /locus_tag="ACHUQ7_000657"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFR1134204.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKVGH"
     gene            complement(4855..5679)
                     /gene="thiK"
                     /locus_tag="ACHUQ7_000658"
     CDS             complement(4855..5679)
                     /gene="thiK"
                     /locus_tag="ACHUQ7_000658"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFR1134205.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(5660..6301)
                     /gene="lpoB"
                     /locus_tag="ACHUQ7_000659"
     CDS             complement(5660..6301)
                     /gene="lpoB"
                     /locus_tag="ACHUQ7_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFR1134206.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSCASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(6316..6693)
                     /locus_tag="ACHUQ7_000660"
     CDS             complement(6316..6693)
                     /locus_tag="ACHUQ7_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFR1134207.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTIYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(6696..7055)
                     /gene="hinT"
                     /locus_tag="ACHUQ7_000661"
     CDS             complement(6696..7055)
                     /gene="hinT"
                     /locus_tag="ACHUQ7_000661"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFR1134208.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            7388..9573
                     /gene="fhuE"
                     /locus_tag="ACHUQ7_000662"
                     /pseudo
     CDS             7388..9573
                     /gene="fhuE"
                     /locus_tag="ACHUQ7_000662"
                     /inference="COORDINATES: protein motif:HMM:NF007447.0"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(9633..11066)
                     /gene="ptsG"
                     /locus_tag="ACHUQ7_000663"
     CDS             complement(9633..11066)
                     /gene="ptsG"
                     /locus_tag="ACHUQ7_000663"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFR1134209.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLLPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(11361..12158)
                     /locus_tag="ACHUQ7_000664"
     CDS             complement(11361..12158)
                     /locus_tag="ACHUQ7_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR1134210.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(12169..13173)
                     /gene="holB"
                     /locus_tag="ACHUQ7_000665"
     CDS             complement(12169..13173)
                     /gene="holB"
                     /locus_tag="ACHUQ7_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFR1134211.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSCLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(13170..13811)
                     /gene="tmk"
                     /locus_tag="ACHUQ7_000666"
     CDS             complement(13170..13811)
                     /gene="tmk"
                     /locus_tag="ACHUQ7_000666"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR1134212.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(13801..14823)
                     /gene="yceG"
                     /locus_tag="ACHUQ7_000667"
     CDS             complement(13801..14823)
                     /gene="yceG"
                     /locus_tag="ACHUQ7_000667"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFR1134213.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRVHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(14826..15635)
                     /gene="pabC"
                     /locus_tag="ACHUQ7_000668"
     CDS             complement(14826..15635)
                     /gene="pabC"
                     /locus_tag="ACHUQ7_000668"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFR1134214.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIL
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(15755..16996)
                     /gene="fabF"
                     /locus_tag="ACHUQ7_000669"
     CDS             complement(15755..16996)
                     /gene="fabF"
                     /locus_tag="ACHUQ7_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFR1134215.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(17083..17319)
                     /gene="acpP"
                     /locus_tag="ACHUQ7_000670"
     CDS             complement(17083..17319)
                     /gene="acpP"
                     /locus_tag="ACHUQ7_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFR1134216.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(17530..18264)
                     /gene="fabG"
                     /locus_tag="ACHUQ7_000671"
     CDS             complement(17530..18264)
                     /gene="fabG"
                     /locus_tag="ACHUQ7_000671"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFR1134217.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(18277..19206)
                     /gene="fabD"
                     /locus_tag="ACHUQ7_000672"
     CDS             complement(18277..19206)
                     /gene="fabD"
                     /locus_tag="ACHUQ7_000672"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFR1134218.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGSAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDAPIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(19222..20175)
                     /gene="fabH"
                     /locus_tag="ACHUQ7_000673"
     CDS             complement(19222..20175)
                     /gene="fabH"
                     /locus_tag="ACHUQ7_000673"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFR1134219.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLDIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(20243..21313)
                     /gene="plsX"
                     /locus_tag="ACHUQ7_000674"
     CDS             complement(20243..21313)
                     /gene="plsX"
                     /locus_tag="ACHUQ7_000674"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFR1134220.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(21394..21567)
                     /gene="rpmF"
                     /locus_tag="ACHUQ7_000675"
     CDS             complement(21394..21567)
                     /gene="rpmF"
                     /locus_tag="ACHUQ7_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFR1134221.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(21619..22140)
                     /gene="yceD"
                     /locus_tag="ACHUQ7_000676"
     CDS             complement(21619..22140)
                     /gene="yceD"
                     /locus_tag="ACHUQ7_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFR1134222.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            22339..22923
                     /gene="yceF"
                     /locus_tag="ACHUQ7_000677"
     CDS             22339..22923
                     /gene="yceF"
                     /locus_tag="ACHUQ7_000677"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFR1134223.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKSQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(23035..23994)
                     /gene="rluC"
                     /locus_tag="ACHUQ7_000678"
     CDS             complement(23035..23994)
                     /gene="rluC"
                     /locus_tag="ACHUQ7_000678"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFR1134224.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            24567..27758
                     /gene="rne"
                     /locus_tag="ACHUQ7_000679"
     CDS             24567..27758
                     /gene="rne"
                     /locus_tag="ACHUQ7_000679"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFR1134225.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPEHQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQVPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPMVEPQEETADADIEEVAETAEVVVAE
                     PEVVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDW
                     QRPTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(27955..28897)
                     /gene="flgL"
                     /locus_tag="ACHUQ7_000680"
                     /pseudo
     CDS             complement(27955..28897)
                     /gene="flgL"
                     /locus_tag="ACHUQ7_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
     gene            complement(28909..30551)
                     /gene="flgK"
                     /locus_tag="ACHUQ7_000681"
                     /pseudo
     CDS             complement(28909..30551)
                     /gene="flgK"
                     /locus_tag="ACHUQ7_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(30617..31558)
                     /gene="flgJ"
                     /locus_tag="ACHUQ7_000682"
     CDS             complement(30617..31558)
                     /gene="flgJ"
                     /locus_tag="ACHUQ7_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFR1134226.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(31558..32655)
                     /gene="flgI"
                     /locus_tag="ACHUQ7_000683"
     CDS             complement(31558..32655)
                     /gene="flgI"
                     /locus_tag="ACHUQ7_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFR1134227.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(32667..33365)
                     /gene="flgH"
                     /locus_tag="ACHUQ7_000684"
     CDS             complement(32667..33365)
                     /gene="flgH"
                     /locus_tag="ACHUQ7_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFR1134228.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(33418..34200)
                     /gene="flgG"
                     /locus_tag="ACHUQ7_000685"
     CDS             complement(33418..34200)
                     /gene="flgG"
                     /locus_tag="ACHUQ7_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFR1134229.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYIRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(34338..34925)
                     /gene="flgF"
                     /locus_tag="ACHUQ7_000686"
                     /pseudo
     CDS             complement(34338..34925)
                     /gene="flgF"
                     /locus_tag="ACHUQ7_000686"
                     /inference="COORDINATES: protein motif:HMM:NF009280.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
     gene            complement(35027..35089)
                     /locus_tag="ACHUQ7_000687"
                     /pseudo
     CDS             complement(35027..35089)
                     /locus_tag="ACHUQ7_000687"
                     /inference="COORDINATES: protein motif:HMM:NF012674.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(35109..36248)
                     /locus_tag="ACHUQ7_000688"
     CDS             complement(35109..36248)
                     /locus_tag="ACHUQ7_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFR1134230.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLLNTQGLQLTGYPVTGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVDGAMANNIATGANNIVATTQNGYKPGDLVSYQINDDGTV
                     VGNNSNEQTQLLGQIVLANFANNEGLASEGDNVWSATQSSGVALLGTAGTGNFGTLTN
                     GALEASNVDLSKELVNMIVAQRNYKSNAQTIKTQDQILNTRVNLR"
     gene            complement(36273..36968)
                     /gene="flgD"
                     /locus_tag="ACHUQ7_000689"
     CDS             complement(36273..36968)
                     /gene="flgD"
                     /locus_tag="ACHUQ7_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFR1134231.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGSLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(36980..37383)
                     /gene="flgC"
                     /locus_tag="ACHUQ7_000690"
                     /pseudo
     CDS             complement(36980..37383)
                     /gene="flgC"
                     /locus_tag="ACHUQ7_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
     gene            complement(37387..37803)
                     /gene="flgB"
                     /locus_tag="ACHUQ7_000691"
     CDS             complement(37387..37803)
                     /gene="flgB"
                     /locus_tag="ACHUQ7_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFR1134232.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            37958..38617
                     /gene="flgA"
                     /locus_tag="ACHUQ7_000692"
     CDS             37958..38617
                     /gene="flgA"
                     /locus_tag="ACHUQ7_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFR1134233.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSEQVVSGVVDADGNILIN
                     L"
     gene            38693..38986
                     /gene="flgM"
                     /locus_tag="ACHUQ7_000693"
     CDS             38693..38986
                     /gene="flgM"
                     /locus_tag="ACHUQ7_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFR1134234.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            38991..39407
                     /gene="flgN"
                     /locus_tag="ACHUQ7_000694"
     CDS             38991..39407
                     /gene="flgN"
                     /locus_tag="ACHUQ7_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFR1134235.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(39447..40982)
                     /gene="murJ"
                     /locus_tag="ACHUQ7_000695"
     CDS             complement(39447..40982)
                     /gene="murJ"
                     /locus_tag="ACHUQ7_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFR1134236.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(41149..42015)
                     /locus_tag="ACHUQ7_000696"
                     /pseudo
     CDS             complement(41149..42015)
                     /locus_tag="ACHUQ7_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
     gene            complement(42017..42664)
                     /locus_tag="ACHUQ7_000697"
     CDS             complement(42017..42664)
                     /locus_tag="ACHUQ7_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
                     /protein_id="HFR1134237.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTAMSNMVDGDLQTRVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(42675..43259)
                     /gene="rimJ"
                     /locus_tag="ACHUQ7_000698"
     CDS             complement(42675..43259)
                     /gene="rimJ"
                     /locus_tag="ACHUQ7_000698"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFR1134238.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            43495..44703
                     /gene="mdtH"
                     /locus_tag="ACHUQ7_000699"
     CDS             43495..44703
                     /gene="mdtH"
                     /locus_tag="ACHUQ7_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFR1134239.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSASAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            44767..45414
                     /gene="grxB"
                     /locus_tag="ACHUQ7_000700"
     CDS             44767..45414
                     /gene="grxB"
                     /locus_tag="ACHUQ7_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFR1134240.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYVNKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVANYRDNMAKQTQINLLSSMAI"
     gene            45548..46108
                     /locus_tag="ACHUQ7_000701"
     CDS             45548..46108
                     /locus_tag="ACHUQ7_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR1134241.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            46214..47260
                     /gene="pyrC"
                     /locus_tag="ACHUQ7_000702"
     CDS             46214..47260
                     /gene="pyrC"
                     /locus_tag="ACHUQ7_000702"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFR1134242.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEAVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            47334..47579
                     /gene="dinI"
                     /locus_tag="ACHUQ7_000703"
     CDS             47334..47579
                     /gene="dinI"
                     /locus_tag="ACHUQ7_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFR1134243.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            47869..48123
                     /gene="bssS"
                     /locus_tag="ACHUQ7_000704"
     CDS             47869..48123
                     /gene="bssS"
                     /locus_tag="ACHUQ7_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFR1134244.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            48238..49356
                     /gene="solA"
                     /locus_tag="ACHUQ7_000705"
     CDS             48238..49356
                     /gene="solA"
                     /locus_tag="ACHUQ7_000705"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFR1134245.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            49404..49517
                     /locus_tag="ACHUQ7_000706"
     CDS             49404..49517
                     /locus_tag="ACHUQ7_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HFR1134246.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            49778..50344
                     /locus_tag="ACHUQ7_000707"
     CDS             49778..50344
                     /locus_tag="ACHUQ7_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFR1134247.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALCLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            50348..50923
                     /locus_tag="ACHUQ7_000708"
     CDS             50348..50923
                     /locus_tag="ACHUQ7_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFR1134248.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(50965..52017)
                     /locus_tag="ACHUQ7_000709"
     CDS             complement(50965..52017)
                     /locus_tag="ACHUQ7_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFR1134249.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            52242..53162
                     /gene="lpxL"
                     /locus_tag="ACHUQ7_000710"
     CDS             52242..53162
                     /gene="lpxL"
                     /locus_tag="ACHUQ7_000710"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFR1134250.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            53334..53519
                     /locus_tag="ACHUQ7_000711"
                     /pseudo
     CDS             53334..53519
                     /locus_tag="ACHUQ7_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            53555..>54465
                     /locus_tag="ACHUQ7_000712"
     CDS             53555..>54465
                     /locus_tag="ACHUQ7_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR1134251.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYLDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRAHEESLRKSERVQAAWNRKK
                     ELISEGIKVSRRCPAWLRLNDDRRTFTIIPDKVEVVKRAFDLRLQGLSFWAITRTLND
                     EGHLSLNQYTPKQKGWSDTAVKKLLRNRAVIGCFTPAGREEVQGYYPAIISESLFYRV
                     QQLNTGQYGRASVSSNPLSVNLFRGII"
BASE COUNT        13307 a        14495 c        13434 g        13229 t
ORIGIN      
        1 tttatgggat ctggactggt gaacggaggt gattattttt ataataacct ttcattcaca
       61 gtcaccaggt acaacggcat catggcaact gactcaacac aatgtgtaaa aaaaagccgt
      121 ggccgcccaa aagtgttcga cagggatgcc gcgcttgata aggccatgaa attgttctgg
      181 caacacggtt atgaagcgac ttctcttgcg gacctcgtcg aagcaaccgg agccaaagcg
      241 cccacgctat acgcggaatt taccaacaaa gaggggttat ttcgtgccgt tctcgaccgc
      301 tatatcgatc gttttgccgc taagcatgaa gcacagctgt tttgtgaaga gaaaagcgtg
      361 gagtctgcgc tggctgacta ttttgctgcc atcgccaact gctttaccag caaagacacc
      421 ccggcaggct gcttcatgat caacaactgc accaccctct ccccagattc aggagatata
      481 gccaatacgt tgaaatcacg ccatgcgatg caagagcgca ctttgcagca gtttttatgt
      541 caacgacaag cgcgcgggga aatcccgacc cactgtgacg tgacacatct ggcagaattc
      601 cttaattgta ttattcaggg gatgtcgatc agcgcacgcg aaggtgcatc gctggaaaaa
      661 ctgatgcaga ttgccggaac gactttgcgt ttatggcccg aactggtgaa ataagtcgca
      721 aaaaaggccc ctcagcctga gcgaatgagg ggccaaattg cagagtacag ccttgttata
      781 ctttgttatt caaaatcagt tgcccgttac tatctagcgg gatctgcgta cccggatcgc
      841 ggtccatgcg gattttgccc tgctgatcgc caatcttata ggtcacatca taaccgagca
      901 ttttttctga cttgtcatac accgttttac aacgctgttg cgtagtcgtg taagtatcgc
      961 tttcctggag agagccctgg atctggttac cggcatatcc accacccagc gcccccacaa
     1021 cagtggcgac atctttaccg cgaccaccac caaactgatg ccctatcacg ccaccagcaa
     1081 cagcgccgag caccgacccg gtaatgcgat tttcatcctg caccggtcga cgatgggtca
     1141 ctgtgacgtt gcgacactcc tgacgcggtg ttttaaccgt ttccttgatt ggggttgcag
     1201 aaacaacctg agcgtattgc gggccccgtt caaacacgtt cagactggcc actgccgcta
     1261 cgcccaacgc agctgcgaca ccaatcccga tacccgccaa cattgattta ttcacgggaa
     1321 cacctccttc tttgcagtta tgccaatcat gcaagcgaag gagaaaagca gctatcagac
     1381 agaagatgaa aagcgcggga atgactggaa atacttaagg attcggagga gtctgaagga
     1441 atgcctgatg cgacgcttaa cgcgtcttat caggcctacc gctcgtacca tttgtccagg
     1501 gcggataagg cgtttatacc acatccgcca gtgtacgtcg attaatgcaa cttcaaacgc
     1561 ggacggataa cgcggttaat actccccacc agcatcatta atccggtttt aaagtaacca
     1621 tgcagcgcaa tctgatgcat tcggtatagc gagatatata caaagcgcgc aattcgtcct
     1681 tcaatcatca ttgagccgcg cgtcaggtta cccatcaggc taccaacggt ggagaagttc
     1741 gacagcgata ccagcgaacc atgatcttta tactgataat ttttcagcgg cttaccgttc
     1801 atctgcgcca gaatgttgtt cattgcgcaa gtcgccatct ggtgtgcagc ctgagcacgc
     1861 ggcggaacaa agcccccttc cggacgcggg catgacgcgc agtcgccaat agcgtaaatg
     1921 tctggatcgc gagtggtttg cagcgtcggt tccaccacca gctggttgat acggttagtt
     1981 tcaagaccac cgatatcttt caggaagtct ggcgctttga tcccggctgc ccataccatc
     2041 agatcagcct caatatattc gccatcttta gtgtgcaggc cgccttcatc agcactggtg
     2101 accatggttt gcgtcagcac gcgaacgcca agtttcgtta gctcgttgtg ggccgcagca
     2161 gagatacgtg gcggtaatgc aggcaaaata cgttctcccg cttctaccag cgttacgttc
     2221 agggcttcgt tggtcaggcc tttgtaaccg tagctgtgca gttgcttgat cgcgttgtgc
     2281 aattcagcgg agagttctac acccgtcgcg ccgccgccga caatcgcaat gttcactttg
     2341 ccatttgcgc ccaggttggc agagtatttc aggaacagat tcagcatctc ctggtggaag
     2401 cgacgcgcct ggtgcgggtt atcgaggaaa atgcagttct ctttgacgcc tggcgtattg
     2461 aaatcgttag aggtgctacc cagcgccatg accagggtgt cataggcgat tttacgttcc
     2521 ggaaccaaca gttcaccttt ctcgtcgcgt agttctgcaa tagtgattgt tttcgcttca
     2581 cgatcaatat caatgacgga acccaactgg aactggaaac catgattgcg cgcatgggcc
     2641 agatagctca acgcatcgac gccttcatca agcgagccag tcgccacttc gtgcagcagt
     2701 ggtttccaca ggtggctgtg gttacgatcg accagcgtaa ttttggcttt tttcttacgt
     2761 cccagcttat gccccagctg tgttgccatt tccagcccac cagcaccgcc gccgacaatc
     2821 acaatctttt tcaatggcgt agtcaacgtg ccccccttaa atttattaac caattgttaa
     2881 ttaatggtta ttaacatagc tcttaattaa caacaggtta cagcaatgaa aatgttcttc
     2941 gctattgaga ataacatgaa tggtgcattg gtcataccaa aattgatgtt aatcaagttt
     3001 tgttgcttaa aagataggca actttaccgg gggagatcaa aaaatagagt ttattgttta
     3061 ttatttgagc aaattcggaa gggtaatata gggtgaagcg tatatcgagg gcgtttggtc
     3121 gtcctgataa gatgcgcagc atcgcatcag gcattgatgc tcgactgtcg gatgcggcgt
     3181 ggacgcctta tccgacttac agaattcgga gcgcatcagg taccggcaag gttacctgat
     3241 gcgacgaggg catttacccg agggttttga acgctttaat gcgctgtaaa tgcggggaga
     3301 tattcttgaa tttgtgcgtc tgctcttcgt cccagacaat ctcgtaataa tgatgcaact
     3361 cttcagatgt ccgctggctg ttaagtgctt catcattacg cgacaaaata accagacaac
     3421 gatcgcgatt cttctcgcgg aagttggtca cacacttagt ggcaatatcg gcatactctt
     3481 ccgggcgatc aatcttgcct tccatgttct cataagggaa caaattaggg ttgaagatca
     3541 cctggcggat atcgcaaaga aaaccaatcc gttccgccca gtatccgccc aggccaacgc
     3601 cgcaaattag cggacgctcg tcaacgttca gttgcaacat tttgtccact tctttaagca
     3661 gatgctgcat atcatgtttc ggatgccgcg tactgtagct tatcaagcgt acatccgggt
     3721 caataaactg caattgtaag actttctcgt ggttacccgg actgttagag tcaaaaccgt
     3781 gtaaatagat aatcatcgct tcctcacata agccagggtt agtgacctac tttctcttcc
     3841 tgccagcgtt catgtaactg attcagacgg gtgctgatcg ctttccagcg agccgagtcc
     3901 atcagttcct gtcgcgaaaa tgaaccttta tgatacaaac gtgtaacacg ttctgccttg
     3961 atcggtgaca gattatctaa cacgctgacg gcccctttac gattattgca gaccaggatc
     4021 atatcgcaac ccgcatccag tgatgcctgc ccgcgttcgg cataactgcc cataatcgcg
     4081 gcaccttcca tcgataaatc gtcagagaaa atcacgccgt caaaccccag ttcctgacgc
     4141 aaaacggttt tcagccagta gggagaaccg ctcgccggac gcggatcaac atcactgtag
     4201 atcacatgcg caggcataat ggcgtcgagt ttattttcgc ggattaagga actgaagacc
     4261 gacatatctt tagcgcgaat ctccgcttgt ggacgtggat cgcacggtgt ttctttgtgt
     4321 gagtctgccg ttactgcacc gtgtcctggg aagtgtttcc cggtcgtttt cattccggct
     4381 tcatgcatac catcaataaa ccggctggca attgccaggg ctttttgtgg atcggcatga
     4441 taagaacgct cgccaatcgc cgcgctgata tgcccgacat ccagcactgg cgcaaagctg
     4501 atatcaatat ccatagcgat catttcgctg gccatcagcc aacccgcctc ttgcgccagt
     4561 ttgccgccct cttccattcc caacagcgca gcaaaggatt gtgctgccgg taagcgggta
     4621 aaaccttcgc gaaaacgctg cacgcgtcca ccttcctgat ctaccgccac caccagatga
     4681 ttgcgcgatg ctgcgcggat ctggcgcacc agttcacgta actgggcagg atcatgatag
     4741 ttacgcgtaa agagaatcag ccctcccacc agcggatgcg ccagtatttc acgctcttcc
     4801 gcgtccagtt cgtaaccttc gacatccaac attactggac ccacactgct ctccttattg
     4861 ttttattaat agctgccgcc aggtgtcatc ggccagcctg ataaattgtt gatcgccggt
     4921 ttgtcgccag cggtactcaa accaccctcc tttgagcatc agcagccagg gaaaccatcg
     4981 cctgacctga cgccataatt gcgccggata aatcttcgcg cgagtggcat agtcattgac
     5041 caattgccgg tgctgttcag tattttccac ccacaccgcc gccagttcca gcgcgatatc
     5101 accatctcca gcatactccc agtcgatgag ttttaaccct gatgcgctat gcactaaatt
     5161 tccggcgtgg acatccatat gcaatggact taagcgtaaa ggtcgtggtt cccgcgcttt
     5221 gcgcagacgt tttaacattc gcagccaacc cacagtccgc cgcgccggat cgctttgctg
     5281 ccagtacagt tccagtaagg gcaacagcgt tattcgccag ccaaaacgtg gttgttgatg
     5341 tagataatac agcaagcctg ccagttcgtt ggtatccggc aaatacgttt ttaccgcacc
     5401 gggcagatag tcgactacca tccagtcacg gagatataaa tgcggcttcg gtgcaatgct
     5461 tgcgggtagt tgtgataaag cccgatactg gcgcaagaac gcggactgcg gcgcatcagg
     5521 atcgtgcggc tgacgcacaa caaaacgctg gccctgatgt tcaatgagaa aactaccgcc
     5581 actaagccca ctattaaacg tcgtgacggg atgaaactgc gggaaaaagc gcgacagcaa
     5641 ttcgtcgcgc gtgatgggat tattgctgcg aaacggcacc tttacctgac cagataattt
     5701 cgcccgtctg caccagcatc agctgcattt gtagggtcgg agcgttaacg ttgccagagg
     5761 cgcaggagta cagcacgtaa tgagcgccga cattgcgggc aatgcctatg gctttactac
     5821 gggtgcctaa actgtcctgc ggcgacaaac ctaactgttg cttagccatc gacagttgct
     5881 gggcggaaac cagggtaaat ttcccgttat tagccagcgc atttcgcagc gtttcggtcg
     5941 cttctgcggc attcagcgaa ccgttagtac ggttgttaac gctatcaacc agcaggacgc
     6001 tacccgcagt caccccgtca gccccaagca tcttactgac catcggctgc attgcgccat
     6061 tccagtcata atggcgaata tgcggcgcag gcggtgccgt tcgatcttcg tgctcaattg
     6121 ggcctggctg ctgcgggatc gtcggcaccg agggcactgt ggggacaggc tgttgtggct
     6181 cggctggttg ttccggcgct ggtttcactt cttctaccgg tgcaggttca cgttgcccca
     6241 cacacccggc gagaaacatc gccagcgcgg taatcaaggc gtagcgactc atttttgtca
     6301 tcaagattca cccctttaca aatatagata aagtctgact ttgtgcgccc ccagaaaatt
     6361 ggcgctgccg tacagcgtta ccgccgaatg tgcgggaatg gtaacgctgc gtggcctttc
     6421 cagaggatgc atctccagcc ctctggcgtc ataccagtaa aaacgataat gaacggtaac
     6481 gggttcttgc ctttcgttat aaatcgttga ggatgctgaa ggttgaatat cagacgtcga
     6541 aaggacgggc ttttctgcac tgatgcccgc agccaataaa gatgactcca tcaccagcga
     6601 ttgctcatca ttcaccggaa tttccggatg tgaacgacag cccaccagca gtaacaacac
     6661 cagagacacc agcccaaagc atccttttct catcgttaca gacctttatg cgccagcatt
     6721 ggtcccagcg gacggccacc caacaagtgc atatggatgt ggtaaacctc ttgtccgcca
     6781 tggcggttgg tattcatgat cagacgatag ccatcttcgg caataccttc ttgctcagca
     6841 atttttgccg ctacggtgat catgcgtccc agcgcctgct catgctcagc tgacacgtcg
     6901 ttcacagtcg gaatgagaat attcggaatg atcagaatat gcgttggcgc ttgcggcgaa
     6961 atatcgcgaa acgccgttac cagatcatcc tggtagacga tatcggaggg gatctcacga
     7021 cgaataattt tgctgaatat agtttcttct gccacgacat tttccttttt caaatagcca
     7081 tgcgttgcgc catgctacgc cgcgccacac tgcgagtata gagtatggtc gagttaaacc
     7141 tgctctttca atcttaactc acgatttctt aagcaaaaaa tcgttttgct gccgtatata
     7201 tcgccattat tcccatttct gcctgcgaat gcgctgaaaa gacattctct gtttggttta
     7261 cacgacaaat atgaatgcgt atatttctca tttgcatttg cagcaaaatt attcgcacac
     7321 taaatataac cgctcatcac cctcctccgg catgagcctg acgacaacat aaaccaagag
     7381 atttcaaatg ctttcaacac aatttaacag ggataatcaa tatcaagtca tcaccaaacc
     7441 gtcactactt gccggttgca tagcactggc actattacct tctgccgctt ttgctgcacc
     7501 agccactgaa gaaacggtga ttgttgaggg ttcagccaca gctccagatg atggcgaaaa
     7561 tgattacagc gtaacgtcta cctctgcggg taccaaaatg cagatgactc aacatgatat
     7621 tcctcagtcg gtcactattg ttagccagca gcggatggaa gatcagcagt tacaaacgct
     7681 gggcgaagtg atggaaaaca cgctggggat cagcaaaagt caggcggatt ccgatcgtgc
     7741 tctttattat tcccgcggat tccagatcga taactatatg gttgatggta tccccaccta
     7801 ttttgaatcg cgctggaatc tgggcgacgc actttctgat atggcactgt ttgaacgcgt
     7861 agaagtagtg cgtggcgcga caggactcat gaccgggacg ggtaatccat ctgcggcaat
     7921 taatatggtt cgaaaacacg cgacctgtcg tgaatttaaa ggcgatgtct cggcggaata
     7981 cggtagctgg aacaaagaac ggtatgtggc ggatttacaa agcccactca ccgaagacgg
     8041 taaaatccgc gcgcgaattg tcggcggcta ccagaataac gactcatggc tggaccgcta
     8101 caatagtgaa aagaccttct tctcgggcat tgtcgatgct gatttaggcg atcttacgac
     8161 gctttcagcc ggttacgaat atcagcgcat tgatgttaat agcccaacct ggggcggttt
     8221 accgcgctgg aatactgatg gcagcagcaa cagttacgat cgcgcacgca gtaccgcacc
     8281 tgactgggcg tacaacgata aagagatcaa caaggtcttt atgaccctga agcagcggtt
     8341 tgctgatacc tggcaagcga cactgaatgc cacccactct gaagtcgaat ttgacagcaa
     8401 aatgatgtat gtcgatgcct atgtaaacaa agcggatggt atgctggttg ggccatacag
     8461 taattatgga cctggctttg atcatgtcgg cggcaccggt tggaacagtg gcaaacgtaa
     8521 agttgatgcg ctggatttgt tcgctgacgg tagttatgaa ttgtttggtc gtcagcacaa
     8581 tctaatgttt ggtggcagtt acagcaaaca aaacaatcgt tcagttcatg ggccaacatc
     8641 ttcccggatg aaattggcag tttctacaac tttaatggca atttcccaca agccgactgg
     8701 tcatcacaga gcctggcgca ggacgatacc acacatatga aatcgttata tgctgccact
     8761 cgtgtcaccc ttgccgatcc gctgcatctg atcctcggcg cacgttatac caactggcgg
     8821 gttgatacgc tgacttacag catggagaaa aaccacacca cgccttacgc tggtctggtg
     8881 tttgacatca atgacaactg gtcgacctac gccagctata cctctatttt ccagccgcaa
     8941 aatgatcgtg acagttcagg caaatatctg gctccaatca ccggtaacaa ctacgagctg
     9001 ggtctgaaat cggactggat gaatagccgt ctgaccacca cgttagccat cttccgtatt
     9061 gagcaggata atgtcgctca gtccaccggt acacctatcc ccggcagcaa cggcgaaacc
     9121 gcctataaag cggtggatgg gacagtcagt aaaggggtgg aatttgaact caacggcgca
     9181 attaccgaca actggcagct gacatttggc gcaacgcgct atcttgcaga ggataacgaa
     9241 ggaaacgccg ttaatcctaa tctgccacgc accacggtta aaatgttcac cagctatcgg
     9301 ttgcctgtca tgccagagtt gacagtcggc ggtggtgtta actggcaaaa tcgcgtgtat
     9361 accgacaccg tgacaccgta tggcaccttc cgcgccgagc aaggtagcta cgcgctggtg
     9421 gatctcttca cccgctacca ggtgacgaaa aacttctcgt tacaggggaa cgtcaataac
     9481 ctgttcgaca aaacctacga taccaacatg gaaggttcta tcgtctacgg cgcaccgcgt
     9541 aatttcagca ttaccggcac gtatcaattc tgaataacac ctgtaaaaaa ggcagccatc
     9601 tggctgcctt agtctcccca acgtcttacg gattagtggt tacggatgta ctcatccatc
     9661 tcggttttca ggttatcgga tttagtaccg aaaatagcct gaacaccaga accagcaacc
     9721 actacgcccg ctgcgcccag tttcttcagg ccagcctgat ccactttaga cacatcagca
     9781 acgctgacgc gcagacgggt aatacatgcg tcgaggttag taatgttttc tttaccacca
     9841 aatgcagcaa ccagagccgg tgccatttcg ctggtacctg ttgcttttgc atcttcagtc
     9901 gcgtcttcac gacccggcgt tttcagatcc agtgctttaa tcagcacgcg gaagatggtg
     9961 tagtaaacaa tcgcataacc gataccgacg atcggtaaca gccacagttt gctgctgtta
    10021 ccagacagaa cgatgaagtc gatcagaccg tgcgagaacg acgtaccgtc acgcatcccc
    10081 agaagaatac agattgggaa tgccaggcct gccagaatcg cgtggatgat gtacaggatc
    10141 ggcgcaacga acatgaagaa gaactcgatc ggctcggtga taccggtcag gaacgaggtc
    10201 agcgccgcgg agatcataat accgcccact ttcgcgcggt tttctggttt agcagagtgc
    10261 cagatagcaa ttgcggcagc tggcagaccg tacattttga acaggaagcc accagacagt
    10321 ttacccgcag tcgggtcacc cgccatataa cgcggaatgt cgccgtggaa aacctgacct
    10381 gctgcgttgg tgtattcacc aatctgcatc tggaaaggta cgttccagat gtggtgcaga
    10441 ccaaacggta ccaggcaacg ttcgatgaaa ccgtaaatgc caaacgcaac taccgggttc
    10501 tggtaagcag cccactgaga gaaggtctgg attgcagaac caatcggcgg ccaaatgaag
    10561 gacagcacaa cgccagtaaa gatggcagcc aggccagaaa tgatcggcac aaagcgttta
    10621 ccggcaaaga agccaagata ctcaggcagc ttaatacggt agaaacggtt aaacatgtac
    10681 gctgcaatcg caccggagat aatccctccg agtacgccag tatccgccaa gtgtttagag
    10741 gcgatttctt cagcaggtaa atgcagtacc agtggcgcaa ccacggccat ggttttaacc
    10801 atgatgccat aggcaacaac tgcggccagc gcggatacgc catcgttatt ggtaaagccg
    10861 agggcgacac cgatcgcaaa aatcagtggc atgtttgcaa agacggaacc gcctgcttct
    10921 gccataacat gcgatacaac ggcgggcagc cagctgaaat tcgcggaacc gacgcccagc
    10981 agaatacctg cgataggtag tacggatacc ggcagcatca gcgatttacc gaccttttgc
    11041 aggttagcaa atgcattctt aaacataatt gagagtgctc ctgagtatgg gtgctttttt
    11101 tacgttctca cgcgtggcaa ggggggagag cctcgccgtg tacagggcat ctaagcgccc
    11161 tttatttatt acacagagta aaataattca gtgccaattt gtttgacggc tatcacgttt
    11221 caacttcaga tcattggaga agttgcacat tttgacaaaa catatgtcac aatatttcaa
    11281 acgcggttat tcaccgccca attttcggcg gtgaacttta ctcgttttag ccattaatta
    11341 cgagctttaa aaaaactcat tcaacggatg gattgaaggc gggaagcgtc gatgtgaaac
    11401 agacgggcga agttatcggt ggttacctgc gccagttctt caacggcaac acctttcaac
    11461 acagccatgt attctgcaac gtcacgaacc atcgcaggtt gattctcttt tcctcgatgc
    11521 ggtaccggcg caaggtaagg tgagtcagtt tccaccagta atcgatccag ggggacataa
    11581 cgcgcagcat cgcgcagttg ctccgcatta cggaaggtca caatgccgga aaaggagatg
    11641 taaaatccga gatccagtaa tttacccgcc gtttctctgt cctctgtaaa acagtgtagt
    11701 acgccaccgc aatccgtcac tttttcttcg cgcagaattg ccagcgtatc ggcacgggcg
    11761 tcacgggtat ggacgataac cggcttgttc agttcacgac caatctggat atgatggatg
    11821 aaggactctt gctgacgtac tttagtttcc ggtgtgtaat aataatccag cccggtttca
    11881 cccagcgcta caacaccctc ttctgccgcc agacggcgta aatcttctac atcgtaggga
    11941 tcattctggt tgagtggatg cacgccacaa gaaaatacga cgttgtcacg ttcgcctacc
    12001 agatcccgca tatgtaaata acccggtaat gttgtggcga ctgccagaca aaatttcaca
    12061 tcgcgtgcgg cggctttcgc cagaacgtca tccacgtcct tatgcaaaga ttcataatcc
    12121 agaccatcga gatggcagtg tgagtcgact aaaaacatga tgtctctctt aaagatgagg
    12181 aaccggtaac acaacgcccg gttgcaggta atgctcaata cgcagtaaaa gatcggtgat
    12241 gagaagctcg cggttgatgc ctgtgacaga cattaactgt tcacgaatgt ggcaaacatc
    12301 ccccagtata gcctgcaggc acgagggaga aagatggttt gccagttcgg cgaccaggcc
    12361 cggcacatca acattggtca cctgcgcagc gccatgatgg cgttttagcg catccatcag
    12421 caacgttgcc agccagtgta aacgcgccgg agcttgttca tgattaaggg ccgccagcag
    12481 cgaataccag tcgcccgatg gcacgctata tgccaacgcc tgacacaatg tttcacgagc
    12541 ctgccagtta tctccctgaa acaacgccag tgccgcgcca ggcgaaccgg cgcttaagcg
    12601 caatgcggca agtaatgcat cctgtgacat tgtcacttcg cgtgaaagcc aagtcacggc
    12661 gtactgttct ggaggcggcg caaggtaatg taaccgacaa cgactacgta atgttgccag
    12721 taaacgttca ggctcgcggg tagccaggaa aaaccaggtt tctgctggtg gctcttcaag
    12781 cgttttcagc aatgcgttag ccgcggcgtc ggtcagtaag gcagcatcgg ttacccagac
    12841 aactttcgca ccacctaggc gtgcgtgctc attcagcttt tcggtgacct cacgtaccgc
    12901 atcaatgccc agcgtatttt tccctttttc gggagccagg gtgtagtaat cgggatgcgt
    12961 gccagcctgc atcaactgac atccacgaca gtgaccgcaa cttttgtggc cctgcggttg
    13021 ttggcagagt aaataacggc tcagggcgta gattaaagca tcatcgccca tgcccggtaa
    13081 cgcctgaatg agtagcgcat ggtgacctct tccggcctga tagctggcta ccagtttttc
    13141 gaaatcaggt cgtaaccatg gataccatct catgcgtcca actccttcac ccagtgggtc
    13201 acggtagtgc ggattgcatc catcacggcc tccagcggct gggtggcatc aatggtatga
    13261 atacttttat cttgtgcagc cagttccaga tagcgggcgc gggtgcgatt aaagaaatcg
    13321 aaagactctt gctcaatacg atccagctcg ccgcgcgcac gcgcgcgttt taagccaact
    13381 tccggggtaa catcgagata gagcgttaag tctgggcgaa aatccccgag aacagcatca
    13441 cgcagtgttg ccagcatatg ttggtcaata ccacgtcctc cgccctgata cgcctgagtg
    13501 gaaagatcgt ggcgatcgcc aatcacccag gtgccgttag ccagcgcagg tttgatgacc
    13561 gtttctacca gttgaacgcg cgcggcataa aacatcagaa cttcggcttt atcggtaatg
    13621 acttcatcgc ctaccgattt gatatccagc accaggcttc ttaacttttc ggcaagttgc
    13681 gtaccgccag gttcccgagt gaaaaccatg tcgcggatac ccagttgctc gagcgtctca
    13741 accaccacat tacgcgcggt agttttgcct gcgccttcca gcccctcaat gacgatatac
    13801 ttactgcgca tttttttcct taagcacttt cagataatcc tgcacagact tgttatgact
    13861 ggcaagattg gtattaaacg tgtgaccacc tttaccatcg gccacaaaat agagatacgg
    13921 cgtttttgcc ggatgcgcag cagccttcag cgaatccgcc cccggcgtag ctatcgcacc
    13981 cggcggcaaa ccggtaatgg tataggtgtt atacgctgtc ggcgtttcca ggtctgcacg
    14041 agaaagtttg ccattataac gctctcccat cccgtaaatc acggttgggt cggtctgcag
    14101 gcgcatacca atgcgtaaac ggttgataaa taccgaggca acctgatcgc gttcactggc
    14161 aacggcggtt tctttttcga taattgatgc catcgtcacc agctgatttt tatctttata
    14221 aggcagaccg tccgcacgcc cttcccaggc gctatcgacc gctttcacca ttttcttgtg
    14281 aactcgcttg agtaacgcga catcggtggt attggcggta tacatcaagg tgtctggcca
    14341 gaaccaacct tcaatccact ccgggttttc cagttcaagt gcctgcgcta cggtggcgta
    14401 cttatcgtca ctcagcgtgt gcttgatata cggagcctca cgcaattgct tgaggtagtc
    14461 gctcagacgc atcccttcta ccagtcgcag cgggaactgt gcttctttac cgctttccaa
    14521 caatttcagc atctcgcgca cggtcatctg cggtgtaaag cggtaagtcc cggctttaaa
    14581 gtgagaaaga tccggttcga tacgcagcag ccattgaaaa acccgtgggc gattgatgat
    14641 cttatcagca taaagctgtt caccgagcgc cagacgtccg gtccctggct tcagggtaaa
    14701 tatcgtctct tctttgataa gcaatttgct gtcggcaaga tggcgaacct tccagacgcc
    14761 cacaccagcg gcgataccca gtaccaccag caataacaag ataatcaata acactttttt
    14821 catgactaat tcgggcgctc acaaagtggg gctaaatatt catataacgt tgctgacgaa
    14881 aaggtgacat cgccacaggc acgtacgggc atcactggca ttaacgcatt acaaataacc
    14941 atctcatctg cctgcaacgc ctcctccagc gaggcttgca cttcgacaag ctgataagag
    15001 gattgtgcca gtaaacggat acagaattgt cgcataatgc cgttaacacc tgcctgatcc
    15061 agtcgcggcg tataaactac attgcccttc cgccagaaca aattagccgc acagcattcc
    15121 gtaacccacc cttcgctgtc aaggaccagc gcctcatcag cgtttgtctg ctcaagatga
    15181 gagcgaatca atacttgctc aagccgatta agatgtttaa tacctgcaag atgaggattg
    15241 cgccccagcc gcaccgggct tagcgccaac gtcatccctt cgttacgcaa acggtcgtaa
    15301 tgtgcaggat aagccgtaac ggagagaatc cgcgttgctg gtccgctgtt caatgtgctg
    15361 taccctcgcc cgccactacc gcgactgatc acgactttca gcacaccatt ttgctgttct
    15421 gctgccagcg ttttcatctc ttgttcaagc tgaggccaga agtcacagga aatcatcaac
    15481 cgctgacaag catcctgtag tcgcaggata tgcgccgata acaaactgac tttaccgtcg
    15541 ataactctgg cggtggtaaa acaaccatca ccaaactgcg ttgcccgatc gcttactgcc
    15601 agcgattcct gcttataacc gttaattaag aacataccgg ctccttatca gagaattgtt
    15661 agtgtggcag catgttcact acggaacaag tcggaataaa agctaagaaa aaaggcccgc
    15721 aagcggacct tttatatggg tggaaaatga caacttagat ctttttaaag atcaaagaac
    15781 cattagtgcc accgaagccg aaggagttac acagcgtgta ttccattccg ctgacctgac
    15841 gtgcttcatg cggtacgaaa tccagatcgc aaccttcatc cgggttatcc aggttgatgg
    15901 ttggcggaac agcctgatcg cgcagcgcca ggatggagta gatagattct actgcacccg
    15961 ccgcacctaa caggtgaccg gtcatagatt tcgtggagct taccaacaca cggcttgcag
    16021 cttcaccgaa gatggttttc accgcctgcg cttcagcttt atcgccagcc ggcgtagaag
    16081 taccgtgcgc gttaacgtag ccaatctgac tcgcttcaat gcctgcatca cgcagagcat
    16141 ttgccattgc cagagctgcg cctgcgccat tttctggcgg tgacgtcata tgataagcat
    16201 cgctgctcat accaaagccg acgagttcag cgtaaatttt cgcaccgcgt tttttcgcgt
    16261 gttcgtactc ttcaagtacc agcataccgg caccatcgcc cagtacgaag ccatcacgct
    16321 ctttatccca cgggcggctc gccgcttgcg ggttatcatt gcgggtagat aatgcacgtg
    16381 ccgcgccaaa accaccaacg cccagcggcg tactggcttt ctctgcgcca cctgcaacca
    16441 tcacgtcagc atcgccatac gcgataatac gcgcagcatg gccaatgttg tgcacgccgg
    16501 aagtacaggc ggtcgcgata gagatgcttg ggccacgcag gccatacatg atagtcagat
    16561 gacctgccac catgttcaca atcgttgacg gaacgaagaa tgggctgatc ttacgtggac
    16621 caccgttcat cagagatgtg tggttttctt cgatcagtcc gaggccgcca atcccggagc
    16681 caattgcggc accaatgcgg gttgcgttct cttccgttat ttcaaggcca gaatcctgca
    16741 tggcctgaac gccagcgaca attccatatt gaatgaaggc atccatcttg cgctgttctt
    16801 tgcgcgagat aatgtcctca cagttaaaat cctttactaa gccagcaaat ttcgttgcat
    16861 aggcgctagt atcgaaatgg tcgattaggc tgatgccact ctgaccggca agcagagctt
    16921 tccaggtaga ctctacggta ttgccgacag gagacaacat gcccagtccg gtcacaacta
    16981 cacgacgctt agacacgttt gtcctccaag gagggaaaaa tgattctagt gggacaaaaa
    17041 gataaaactc aggcggtcga acgaccgcct ggagatgttc acttacgcct ggtggccgtt
    17101 gatgtaatca atggcagcct gaacggtggt gattttctca gcttcttcgt ccggaatctc
    17161 agtatcaaac tcttcttcca gagccattac cagctcaacg gtgtcaagag aatccgcgcc
    17221 caggtcttca acgaaagaag cattgttggt aacttcttcc tgcttaacgc ccagctgttc
    17281 gccgataatt ttcttaacgc gttcttcgat agtgctcata ctcttaaatt tcctatcaaa
    17341 actcgctttc gcgatggttt tcgtagtgta taaaatgttg aaaaatttgc aactaaatcc
    17401 cggcaggtct taccacgatt ttacgttatt ttgaggcctt accccccaat aacgcaaatc
    17461 attttgcact aattattact cataaccacg cagactgcgc gcaacatgag cattttgtgc
    17521 aaatcgcggt cagaccatgt acatcccgcc gttcacatgc aaagtttcac ccgtgatgta
    17581 agctgcttcg tcggatgcca ggaatgcaac cgcgttggcg atttcctgtg cgccgccgag
    17641 gcgacccgca ggaacctgcg ccaggatacc cgcacgctgg tcatcgctca gcgcacgtgt
    17701 catgtccgtt tcaataaagc ccggagcaac aacgtttaca gtaataccgc gtgacgcaac
    17761 ttcgcgcgcc agtgatttac tgaagccgat caagcccgct ttcgccgcag cgtagttggc
    17821 ctgaccgcca tttcccatgg taccaaccac agaaccgata gtgataatac gaccatgacg
    17881 ctttttcatc atagcgcgca ttaccgcttt tgacagacgg aaaacagatg aaaggttagt
    17941 ttcgataata tcgttccact cttcatcttt cattcgcatt aacaggttat cacgagtgat
    18001 accggcatta ttgaccagga tatccacttc accaaattct gcgcgaattt tttccagaac
    18061 agattcgata gatgccgggt cggtcacatt caacatcaga cctttgccgt tggcacctaa
    18121 ataatcactg atcgcctgag cgccattttc actggtcgca gtgccaataa ctttcgcgcc
    18181 acgggctgcg agcgtttcag caattgcgcg gccaattccg cggcttgcac cggttaccag
    18241 tgcgattttt ccttcaaaat tcatgatttt cctcttttaa agctcgagcg ccgctgccat
    18301 cgctgaaggt tcgttcagcg ccgaggcggt cagggtgtcg acaatgcgtt tcgtcaggcc
    18361 agtaagcacc ttgcccgggc cgacttcata gagatgttct acgccttgcg ctgccatgta
    18421 ctcaacagac ttcgtccact gaaccgggtt atacaactga cgtaccagtg cgtcacggat
    18481 ggcatcacca ttggtttcgc atttcacatc aacgttattc acaacagaaa ctgttggtgc
    18541 gttaaaggtg attttcgcta attctactgc cagtttgtcg gctgctggtt tcatcagcgc
    18601 acagtgagac ggtacgctca ctggtaacgg cagcgcgcgt tttgcgccag cagctttaca
    18661 ggcagcgcca gcacgctcaa ccgcttcttt atgaccggca ataaccacct gtcccggaga
    18721 gttaaagttt accggagaaa cgacctgacc ttctgcagct tcttcacacg ctttcgcaat
    18781 aggcgcatca tccagaccga tgattgccgc catagcgccc gtgccttccg gtacggcttc
    18841 ttgcatgaac ttaccgcgca tctcaaccag acgcaccgca tcagcgaaat caatcacgcc
    18901 agcgcaaacc agcgcggagt attcccccag gctgtgaccg gccatcattg ccggtgcttt
    18961 accgccctgc tgctgccata cgcgatacag cgcaacagat gcagtcaaca gcgctggctg
    19021 ggtttgccag gttttattca gttcttcagc tgacccctgc tgggtcagcg cccacaggtc
    19081 gtagcccagc gccgcagaag cttcagcaaa cgtttcttcg acaattggat agctcgccgc
    19141 catatcagcc agcattccaa cggtttgaga accctgtcca gggaacacaa atgcaaattg
    19201 cgtcatgttt taatccttat cctagaaacg aaccagcgca gagccccaag tgaatccacc
    19261 gccaaaggct tcaagcagaa ccaactgccc cggcttaatg cgcccgtcgc gtacagcttc
    19321 atccagcgcg cacgggacag aggccgcaga ggtattaccg tggcgatcca gcgtcaccac
    19381 gacattgtcc atcgacatac cgagtttttt cgccgttgca ctgataatac gcaggttagc
    19441 ctgatgcgga accagccagt ccagttgaga acggtcaaga ttattcgccg ccagcgtctc
    19501 atcaacgatg tgcgccagtt ccgttaccgc aaccttgaag acttcgttgc ccgccatcgt
    19561 cagatgaatt gaattctctg gattcacgcg gtcggcgttt ggcagcgtca gcaattcacc
    19621 ataactaccg tcggcatgca gatgggtgga aatgattccc ggctcttcag aggcagccag
    19681 caccgcagcg cccgcgccat cgccaaaaat aataatagtc ccacgatctg ttggatcgca
    19741 ggtgcgcgcc agtacatcgg aaccgacgac cagagcatac ttcaccgccc cagatttcac
    19801 gtattgatcg gctacgctta atgcataggt gaaacctgca caggctgctg caacgtcaaa
    19861 tgccgggcaa cctttaatgt ccaacatgct ttgaatctga caagctgcgc tcgggaaagc
    19921 gtgcgtagca gaagtcgttg ccacaacgat caggccaatc tggtctttct caatgcccgc
    19981 catctcaatt gcgcgtgtcg ccgcttcaaa gcccatggtt gaaacggttt cgtttggcgc
    20041 ggcaatgtgg cgttcgcgga taccggtacg agtgacaatc cactcgtcag aggtgtccac
    20101 cattttttcc aaatcggcgt ttgtccgcac ttgttcgggc agatagctgc cagtaccaat
    20161 aatcttcgta tacatgtacg ctcagtcact tttcggttat ataccgtcac ttgcaaactg
    20221 cgagttcgct ggcagcgtcc tgctaccgca gagttccgct tttgccaccg tccagcagct
    20281 caaaaccagc tgggtataca gattccaggc gagcggcaat tcgctgagga acttgtcgct
    20341 gcaccgcctg cactgcctgt tcaatcgcga ccgcaaaagc tcgctgattg gctgcaccat
    20401 gactttttat caccgtgccg cgcaatccta acagacaggc gccgttatac tggtcggggt
    20461 tgaggtgact gaatcgcctc gtcaggctct tttgtagcca acgctttaat aacagtagcc
    20521 accacgaccg ttttttccct tcaccctgag atttcagcag agaaaggaac atcctgacaa
    20581 caccttccat cgtctttaac gtgacatttc ctgtaaagcc gtcacaaacc agcacatctg
    20641 tcttgccagt taacaactca ttggcttcaa gatagccgat ataattgata gaagggattg
    20701 ttttaagcac cgctgaggca tcccgaatac tgtcaagacc ctttacttct tcttcaccaa
    20761 tattgagcaa cgccacgcga ggattgggaa tttccaccac ctcttcagcc agaactgagc
    20821 ccataatggc aaattgcacc aacattgtgc tgtcacaatc gacgttggcc cctaaatcga
    20881 ggaccaccgt tttgcccttt tgctgatgtg gtaataccgt caccagcgcc ggacgctcaa
    20941 tcccctccag gggcttgagt aataattttg ccagccccat cagtgccccg gtattaccgg
    21001 cactgacaca ggcttgcgct cgaccttctt tcaccagctc cagggccata cgcattgaac
    21061 tcccacggct ggcgcggata gcttgcgaag gccgggcatc actggcgata actgactgcg
    21121 caggaataat ctgcagacgc gaacgttgtt caaagtcagc tttagcaagt aatggcgtga
    21181 tggcgtcggg attgccgact aaaagaagag tgagttgcga attagaattc agtgcctgca
    21241 atgctgcagg cactgtcacg gaagggccaa aatcccctcc catgacatct aacgccaggg
    21301 ttagacgtgt caaggtatcg tcgccgcccg gtttggtctt tccccagttg cctggggaaa
    21361 atcctcacta agcttcatca cgcagacgcg tgattactta gcgatgacct tgcggccgcg
    21421 gtagtaaccg tcggcagtga tgtggtgacg caggtgtttt tcaccagaag ttttgtctac
    21481 agacaggctg gtgactgcgg tcagcgcgtc atgggaacga cgcatgccac gtttggaacg
    21541 ggttggttta ttctgttgta cggccatgga ccttactcct caattacggt ttatccccga
    21601 tccaacgggg agcaccaatt acttacgctt taagctggct aatacggcaa atgggtttgg
    21661 cttttgcact tcttcaggca gttcaccaaa gaccatgtcc gcttcggaca cttcacagtg
    21721 ttcagaatca tgcaccggaa ctaccggcaa ggcgaggatg atttcatctt caaccattgc
    21781 aagcagatcg atttcaccga attcgttaac ctcaatcggt tcatacgctt ccggcagtgc
    21841 ttcagcctgt tcgtctgaac gcacaggact aaaacaatac gttgtgtaga cctgatgagt
    21901 aaacggcttc ccgcaacgct gacactcgag cgttaccgtc accttcgcat cgccgtttaa
    21961 cactgcgaga cgttggttat cgatagcgaa cgacatggag cattccacat cactgtccac
    22021 actgactacg gattcggcga cgcgctcaac ctgatcaggg gtatagatac cctggtaatc
    22081 aaggcgtttt tgagccgtac gaaccggatc gagagtcagg ggtaatttta ccttttgcat
    22141 agggcgcgca tattaacttt gtaacgtcat agagtcaaag aaaaaggcag cctgtggttg
    22201 ccttttgcca ataattcgca cacattgcgg gttactgctt tattttcgtt cagtgctgat
    22261 aacgaacatc aacctgcaaa acgaagcgca tagtttaaaa tggctatcat caatacgcta
    22321 tatctgatgg aaaaaaatat gcctaaactt attttagcct ccacctcgcc atggcgtcgc
    22381 gctcttctgg aaaaactgca aatctctttt gaatgtgcag cacctgaggt cgacgaaacc
    22441 ccacgcagcg acgaatcacc gcgacagttg gtgcttcgac tggcacaaga aaaatcgcaa
    22501 tctctggcgt cacgttatcc ggatcattta attattggtt cagaccaggt atgtgttctt
    22561 gatggcgaaa tcaccggtaa accgttaacg gaagaaaatg cccgtctgca attacgcaaa
    22621 gccagcggca atatcgtcac cttctatact ggactggcgt tgtttaattc ggcgaatggg
    22681 catctacaaa cagaagtgga gccttttgac gtccatttcc gtcacctaag cgaggcggag
    22741 attgataatt acgtgcgtaa ggagcatccc ctgcactgcg cgggtagctt taagagtgaa
    22801 ggatttggca ttacgctgtt tgagcgttta gagggacgtg accctaacac gctggttggt
    22861 ttaccgctaa tcgcgctgtg tcagatgtta cgtcgggaag ggaaaaaccc gctgatggga
    22921 tagttgtttg cgccggatgc ggttacgtcg catccggcaa tcggtgcagg cggcctgatg
    22981 cggcgtaaac gccttatcag gccaaccgcc cattatcagg cttatatgct tgtattagcg
    23041 cgcgttacgc agcttttgca aacaacgctt caaaccttca tccatcggcg cttcgatacg
    23101 catcacctca ccggtccccg gatgggtaaa cttcaacgct gcggcgtgca ggaacagacg
    23161 atttaatccc gtgcctgctt cagtgagctg tctgtcaaat tcacggtcac cgtagcgatc
    23221 gtcaaaggca atcggatgac ccgcatactg tgtatgcaca cggatctgat gagtacgtcc
    23281 ggttaccggg ctacaacgca ccagggtggc aaatgcatag cgttcttcca ctttaaagcg
    23341 tgtttccgac ggtttgcctt cctgactgac acgcacgata cgttcgccgc tttgcagaat
    23401 atttttcaat aacggcgctt gaacgctctt cacatgcgac tgccactgac cgcgcaccag
    23461 cgccaggtaa tctttttgca ttcctttttc acgtaattgc tcatgcagag aacgcaacgc
    23521 cgagcgtttt ttcgctacca gcaacacacc tgaagtgtcc cggtcgagac gatgaaccag
    23581 ttcaaggaac cgcgcttccg ggcgcaacgc ccgcaaacct tcgataacgc cgaagcttaa
    23641 accactgccg ccatgtaccg ccgtaccgga aggtttattc agcaccagga tatgatcatc
    23701 ttcatataag atgacgtccg ccagcgccgc caccttttgc agatgtggcg aaaccgcctc
    23761 ttcttcccgc tcagcaacgc gaaccggtgg gatacgcacc tcatcacccg cttcgagttt
    23821 atattcaggc ttaatacgtt ttttgttcac ccgcacttca cctttacgca aaatacggta
    23881 aatcatactt tttggtacgc ctttcaattg ggtacgcaaa aagttatcga tacgttgccc
    23941 cgcttcgtca gcggtgatag caacaatttt tacggatgga gtctctgttt tcatggtggg
    24001 cgattctaat tagccaacag gattcgcgcc actcattttt ctatgcttat ttttagtttt
    24061 gcaccttatt acttcactac gtgatcactt tattgatggt tattaaacca atcaccagca
    24121 agaagtgaaa aaactgtgag taagcgggtg ataaatggta aaagtcatct tgctataaca
    24181 aggcttgcag tggaataatg aggccgtttc cgtgtccatc cttgttaaaa caagaaattt
    24241 tacggaataa cccattttgc ccgaccgatc atccacgcag caatggcgta agacgtattg
    24301 atctttcagg cagttagcgg gctgcgggtt gcagtcctta ccggtagatg gaaatatttc
    24361 tggagagtaa tacccagtct gtttctttga taattgcgct gtttttccgc atgaaaaacg
    24421 ggcaaccgac actctgcgcc tctttgagct gacgataacc gtgaggttgg cgacgcgact
    24481 agacacgagg ccatcggttc acacccggaa aggcgttact ttgcccgcag cttagtcgtc
    24541 aatgtaagaa taatgagtaa gttacgatga aaagaatgtt aatcaacgca actcagcagg
    24601 aagagttgcg cgttgccctt gtagatgggc agcgtctgta tgacctggat atcgaaagtc
    24661 cagggcacga gcagaaaaag gcaaacatct acaaaggtaa aatcacccgc attgaaccga
    24721 gtctggaagc tgcttttgtt gattacggcg ctgaacgtca cggtttcctc ccactaaaag
    24781 aaattgcccg cgaatatttc cctgctaact acagtgctca tggtcgtccc aacattaaag
    24841 atgtgttgcg tgaaggtcag gaagtcattg ttcagatcga taaagaagag cgcggcaaca
    24901 aaggcgcggc attaaccacc tttatcagtc tggcgggtag ctatctggtt ctgatgccaa
    24961 ataacccgcg cgcaggtggc atttctcgcc gtatcgaagg cgatgaccgt accgaattaa
    25021 aagaagcact ggcaagcctt gaactgccgg aaggcatggg gcttatcgtg cgcaccgctg
    25081 gcgtaggcaa atctgctgag gcgctgcaat gggatttaag cttccgcctg aaacactggg
    25141 aagccatcaa aaaagccgct gaaagccgcc cggctccgtt cctgattcat caggagagca
    25201 acgtaatcgt tcgcgccttc cgcgattatt tacgtcagga tatcggcgaa atccttatcg
    25261 ataacccgaa agtcctcgaa ctggcacgtc agcatatcgc tgcattaggt cgcccggatt
    25321 tcagcagcaa aatcaaactg tacaccggcg agatcccgct gttcagccac taccagatcg
    25381 agtcgcagat cgagtccgcc ttccagcgtg aagtccgtct gccatccggt ggttccattg
    25441 ttatcgacag caccgaagcg ttaacagcca tcgacatcaa ctccgcacgc gcgacccgcg
    25501 gcggcgatat cgaagaaact gcgtttaaca ccaacctcga agccgccgat gagattgctc
    25561 gtcagctgcg cctgcgtgac ctcggcggcc tgattgttat cgacttcatc gacatgacgc
    25621 cagtacgcca ccagcgtgcg gtagaaaacc gtctgcgtga agcggtgcgt caggaccgtg
    25681 cgcgtattca aatcagccat atttctcgct ttggcctgct ggaaatgtcc cgccagcgcc
    25741 tgagcccatc actgggtgaa tccagccatc acgtctgccc gcgctgctcc ggtaccggta
    25801 ccgtgcgtga caacgaatcg ctgtcgctct ctattctgcg tttgatcgaa gaagaagcgc
    25861 tgaaagagaa cacccaggaa gttcacgcca ttgttcctgt gccaatcgct tcttatctgc
    25921 tgaatgaaaa acgttctgcg gtgaatgcga ttgaaacgcg tcaggacggt gttcgctgcg
    25981 tgattgtgcc aaacgatcag atggaaaccc cgcactacca cgtgttgcgc gtgcgcaaag
    26041 gggaagaaac gccgacctta agctacatgc tgccgaagct gcatgaagaa gcgatggcgc
    26101 tgccgtctga agaagagttc gctgaacgta agcgtcctga acaacctgca ctggcaacct
    26161 ttgccatgcc ggatgtaccg ccagcgccaa ccccagctga acctgccgcg actgtcgtag
    26221 ccccagcacc gaaagctgca acggcaacac cagcagctcc tgcacaacct gggctgttaa
    26281 gccgcttctt cggcgcactg aaagcgctgt tcagcggtgg tgaagaagcc aaaccgaccg
    26341 agcaaccaac accgaaagca gaagcgaaac cggaacatca acaggatcgt cgcaaacctc
    26401 gtcagagcaa ccgccgtgac cgtaatgagc gccgcgacac ccgtagtgaa cgtactgaag
    26461 gcagcgataa tcgcgaagaa aaccgtcgta atcgtcgcca ggcacagcag caaactgccg
    26521 agacgcgtga gagccgtcag caggctgagg taacggaaaa agcacgtacc accgacgagc
    26581 agcaagtgcc gcgtcgtgaa cgtagccgcc gccgtaacga tgataaacgt caggcgcaac
    26641 aagaagcgaa ggcgctgaat gttgaagagc aatctgttca ggaaaccgaa caggaagaac
    26701 gtgtacgtcc ggttcagccg cgtcgtaaac agcgtcagct caatcagaaa gtgcgttacg
    26761 agcaaagcgt agccgaagaa gcggtagtcg caccggtggt tgaagaaact gccgctgccg
    26821 aaccaattgt tcaggaagct ccagctccac gcacagaact ggtgaaagtc ccgctgccag
    26881 tcgtagcgca aactgcacca gaacagcaag aagagaacaa tgctgataac cgtgacaacg
    26941 gtggcatgcc gcgtcgttct cgccgctcgc ctcgtcacct gcgcgtgagt ggtcagcgtc
    27001 gtcgtcgcta tcgtgacgag cgttatccaa cccagtcgcc aatgccgttg accgtagcgt
    27061 gcgcgtctcc ggaactggcc tctggcaaag tctggatccg ctatccaatt gtacgtccgc
    27121 aagatgtaca agttgaagag cagcgcgaac aggaagaagt acaagtgcag ccgatggtga
    27181 ctgaggtccc tgtcgccgcc gctgtcgaac ctgttgttag cgcgccagtt gttgaagaaa
    27241 tggctgaagt cgtagaagcc cccgtaccgg ttgccgaacc gcaaccggaa gtggttgaaa
    27301 cgacgcatcc tgaagtgatt gccgccgcgg taactgaaca gccgcaggtg attaccgagt
    27361 ctgatgttgc cgtagcccag gaagtcgcag aacacgcaga accgatggtt gaaccgcagg
    27421 aagagacggc agatgcagat attgaagaag ttgccgaaac tgctgaggtt gtggttgctg
    27481 aacctgaagt tgttgctcaa cctgccgcgc cagtcgtcgc tgaagtcgca gcagaagttg
    27541 aaacggtggc cgcggttgaa ccagagatca ccgttgagca taaccacgct accgcgccaa
    27601 tgacgcgcgc tccggcaccg gaatatgttc cggaggcacc gcgtcacagt gactggcagc
    27661 gccctacttt tgccttcgaa ggtaaaggtg ccgcaggtgg tcatacggca acgcatcatg
    27721 cctctgccgc tcctgcgcgt ccgcaacctg ttgagtaata attagctcaa agtaatcaag
    27781 ccctggtaac tgccagggct tttttatttc atgtttgaat caacatatta aaattcatca
    27841 aatggcatcc ttgctaacca acaatgcaaa ataggcaaaa aaaatgcccg ccccggcgat
    27901 cccagagcgg gcagacaaaa catacccagt ttcatgatat gttttaaagc gagcttattt
    27961 gttgagctgg aagagcgaca atccctgcat atcggtaaat gctttatacg atgcctgcaa
    28021 tgccgtttgc tgcatgatgt aagatgaaat agttgcattc cagtccacat caaccagatc
    28081 gctcatctgc tgcgtttgcc ctaaagcgcg atcgctacct aaggaatcca gcgactccag
    28141 tttgttcagc tgcgtgccta attccgcgcg aacggtaagg acattattca gcgagttttt
    28201 cagtccacgg ttggttttat ccaatgccgc agcggcgatt tctttatccg cttcgctatc
    28261 cgcgaccggc gttttcaggg ctgcgatggc gctatccagc atggcaaaaa gattggtttc
    28321 agaagcgcta ccgtctggtt ccgctaccgc gttactggta atactgtcga aaattttgtc
    28381 gcccgtatgt cctatcacca tcgaacgcga agcatcgacc tgttgcttaa tactttctgt
    28441 tccgccaaca taatcaccgt tagcctcact aaacggcgca gtctctgttt tatatgtagc
    28501 gcccattacc gtcagtggtg ttcgccagat tcagcaactg gtcacgaagc ccctgaatat
    28561 ccgtagccag cgaggcccgg tcatcgtcac tcaaggtgcc attgctggcg tagacaattt
    28621 tttcctgagc attctggata gcagtggtga cctggctaag tacactctct tccagtgaca
    28681 ctttttgagt ggcgaaagta cgtgccagcg tgtactggct gttttgcgcc tgtgcctggg
    28741 agagaacaac agcttgtgat gcagcaatgg gatcgtctga agggttaacg actcgcttac
    28801 ccgtcgacat ctgctcgccg tacttcatcc attctgcctg agaattggtg atgccgcgca
    28861 tgttttgctg gtacatcatc tgggtactga aacgcatttt atctcccctt agcgaatgtt
    28921 aatcagcgca tcaaaaatcg cgtttgccgt ctgcagaacc tgcgcattcg ccaggtaata
    28981 ctgctgaaaa cgttgcagat ttccgtactc ctcatcgaga ttgacaccgg aaatcgactg
    29041 ctgctgattg gaaagctgcg tcaccacatt accttgcgtg gtgctactgg ttttcaacgt
    29101 cgcggtttta ttaccgatat cactcactaa cgaggcataa gcgtcgttaa aggatttcgc
    29161 accgcccacc gttttactgt tgctttgcag atccagcagg gcctgaccgt tgcggttgtc
    29221 gctatcaccc gcatcttctt cgctcgccat cgcgattttc gcttcgtcgg tgattaatac
    29281 atccatgttg acgatggcgt cacttactgg tttcagcgtg aagctgtcgt taacggcagg
    29341 cgttcctgta aacgtcaact ccagaccatc aaatgccact ttaccgttgg catctggtgt
    29401 cgccgtaaaa gtggtattgc tggcaaggcg ggtgacctgc cactgattat tatcgaacga
    29461 gattttgtaa tctgtcgcca gtaccacgga ggcatcagtt accgtggcac cgatcgcaac
    29521 gtcacctttg tttttcgtgt tttgcagaac cgcgggctta ccgatagtaa agaaatcttc
    29581 accagcatcg ccgttagcat caaatccggc tttgtgttgg ctgttgaaag cctcggcaaa
    29641 tgccagcgcc agttgtccaa gcgtattacg cgtctggtcc agatcctgag aacggaatgt
    29701 cagaatgccg cccagcgacc cggtattcag taatttctcc gggatctcaa tattgcctgc
    29761 cgtcccatca acgtaagcga cagtcgtacg agaagggtcg gcgctggaag gaactgccgc
    29821 cagttgccgc gccgtacttc cctgaaccag tgagtaacca ttggcatcgt gatgttataa
    29881 gtaccgccat cctgaacgct gacttctacg ccaacaatct ggtttaattc gctcaccagt
    29941 tgatcgcgtt gatccagcag attgttaggt gacgcccctg cccccacgcc tgtcaggcgc
    30001 gagatttgat cgttcaggct ggcaatttgt ttagcgtaat tgttgatctg atcaacgctg
    30061 gcacctatcg cgatattgac ctgtttgtcc tggtcgcgca gatattgatc ggtggtttta
    30121 aactgattca ccaacccttc tgatttccca atcagcgcct ggcgcgccgc cgggtcttcc
    30181 gcgttactca ccagcgtttg caggctggtg aagaaatcct gcatctgtgt tgccagcgaa
    30241 gaggtactgg tggagagcat attgtcgatt ttcgacatct gctcatagcg ggcggtcagg
    30301 ccgctacttt gcgtctgcgc agcacgtaac tggttggtaa taaacgtatc atactcacgc
    30361 tgcacaccag aaacgtagac gccattgcca acccagccgc cagcgcccaa cgtgctattg
    30421 gcctgcgcca taatagtggt ttggcgggta tatccggcaa cgttatagct ggagatatta
    30481 ttacttgccg tatttaacgc cgcctgggcc gcgttcagtc cgctcatggc gttattgatc
    30541 aagctggaca tggaggttcc ttataagcct tcaattacag agtattatcg gcagcgaccc
    30601 gccggacttg agttattcag aacagattat caatgttcat gctgtaggtt ttgctcacct
    30661 tgtcgcttat cgatttcatc tgctggatca tgttggtgag tttacgggca tagtgaggat
    30721 cggtagcata gcccgcgtcc tgtaggacct gcgccccctg ttccgcactc gcggcggtcg
    30781 tcacggcggc gtagcgcggg ttacgtgtta acagcccaac gtaatccgac aatgcttcca
    30841 gatacgagct gtagacccga aacttcgctt ttactttctt cgcttcgccg ttttcatatt
    30901 cagtcgtggt gatttcagtg acttgccctt tccagttgcc agaggctttg acaccaaaca
    30961 ggttatagct tggctcgccg ttttcgcggc ggatttgccg ttgcccccaa ccagattcaa
    31021 gcgcagcctg agcgaggatc aaatgatgtg gcacaccgct ttgctggctt gccagttggg
    31081 cgggcagcga gagttgcgcg aggaatgctc tactgtcacc cggcagcgaa tcatcgtagt
    31141 tacgtggtac ggccttttgc accagctgcg aaagcgtctg gttttgataa cgcaccacgg
    31201 tttcgagcgg gaatttcatc ggtgctgctg gcgtggactc ctctggcaat ggttgttctg
    31261 gcgtcatctg tttaaccatc atctctgcaa gccccagacc tttgcccgcc gtcatctgtt
    31321 gggcaatttg ctggtcatac atactggtat acagacgagt gtgctcgctg ctgaacaggc
    31381 catcttttgg taaagcgtcg cgcatgcttt tcaacatcat ctgcacgaac atcccttcca
    31441 cctgacgggc caccggacgg atatttgccg ccggatcttc gctcgctttc gcctttagtt
    31501 cgttgagtga ttgcgcatcc caggccgcac ttgccagtag tttgctgtcg ctgatcatca
    31561 gatgatttcc agttttgccc gcagacatcc cgcactttgc attgattgca aaatagacat
    31621 cagatccatc ggcgtagcgc ccagcgcatt gagcgcacgc accacgttat tgaggctggc
    31681 gctggaacgt acgctttgca gcgaaccgcc gctctggcgt aaatcgatct gcgtttgtgg
    31741 agtaacaaca gtctgtccac caccaaacgg tgtatctggc tggctgacat tggcctgacg
    31801 attaactgtt actgagagat ttccctgcgc taccgcgcag ctgtcgaggg tcacttcgcg
    31861 attcatcacc accgaaccgg tgcgcgagtt aatcactact ttagcgtcct gcggggtgac
    31921 attaacctgc atattctgga tatcggcaag gaaacggacc tgggaactgt tgccacttgg
    31981 tacgcgcacc tgaatagtcc gcgcatctaa cgcggtggcg ctgccatatc cacgcacgcg
    32041 gttgatggtg tcagcgattt gctgcgccat gctgaaatct tcgtcgttaa gttgcaaatt
    32101 aagggtattc ccgacgccaa actggctggg caattcacgt tcaataaccg caccattggt
    32161 gatccgtcca ccgttcagtt ggttaacctg aacactgcta ccgcctgcgg aggctcctgc
    32221 gccgccaacc agaatattgc cctgcgccag cgcatacacc tgactgtcaa cgcccttaag
    32281 cggagtcatc aacaacgtac ctccacgcaa gcttttggca ttacccatgg aagaaaccac
    32341 cacatcgatg gtttgcccct gacgtccaaa cggaggaagt gacgctgtca ccattaccgc
    32401 agcgacgttt tttagctgca tattggtgcc cgtcggaacg gtaattccca gctgtgagag
    32461 catgttatta agcgtttgtg tggtaaacgg cgtctgggtt gtctggtcac cagtgccatc
    32521 cagccccact accaagccat agccaatcag tgagttttgc cttaccccct gaacactggt
    32581 gagatcgcga atacgctcag cctgagccgc cgtcgtaacc agtagaagaa ttaatgcaga
    32641 gagaaattta atcaccacag cctcacttac attggcgaca ggttaaggaa gaaacgctgc
    32701 aaccagccca tattttgcgc ttcgttaatg tagccattgc ctacgtattc aatacgcgca
    32761 tccgccacct gagtagacgg tacggtattg ctgccgctga tagtgcgtgg attaaccacg
    32821 cccgagaagc gaataaattc ggtaccctga ttaatggcaa tctgtttttc acccaccaca
    32881 tgcaggttgc cgttgaccag tacctggtca accgtcaccg tcaacgtgcc gctaaaggta
    32941 ttgctggcat tggccccgcc ctttccgttg aacgtgttac caccggaggc ttcgacatcg
    33001 gcacgagcgt taccaaacag cccctgcaaa tagcgcggca cagtatcaaa gccaaaatta
    33061 gttttaccgt cacggctggc attcgcagag gagcttttgc tggcgctgac gttctcctgc
    33121 aacacgatgg tcagcgtatc gccaatattg cgtggtcgac gatcttcaaa cagcggttga
    33181 tagccatagt taatcggctg agcagactgg aaaatagaac cgttggcgac gggcgtcgga
    33241 ccgggaaccg gttgtgcact ggtcgccccc tgcaccagcg gcgtggaggg tatccaggcg
    33301 cagccggtta gtgaaagcac caacaagctg gaaatggcat aagtatgcgc agcgtttttt
    33361 tgcatggcta tcatcttcaa aaatcagtaa accggtgaac ctgccaccgg ttatgcctta
    33421 gagttgcgtc agtttttgca gcatctgatc ggtggtggac accgctttac tgttgatttc
    33481 gtaagcgcgt tgcacctgaa tcatattgac cagttcttcc gccacgttga cgttagacgt
    33541 ttcaacatac ccttgataca gcagtcccgc gccgttcagg cccggcgtgc tttcgttcgg
    33601 tgcaccggag gattgcgttt cggtgtagag gttttcgcca atgctctcca gtccggtatc
    33661 attcataaag gtggtgagat tgagctgccc aacctgaacc ggagctgcct ggccttgttg
    33721 ggttacgctg accacgccat cacgaccgat ggtgatactt aacgcattcg ccggaatggt
    33781 gatcgctggc tgcacctgaa aaccaccagc cgtcaccagc tgcccgttct gatccacctg
    33841 gaaagagccg tcacggatat aggctgatga gccatctggc aacattacct ggaaaaagcc
    33901 ctgcccttta atcgcgacat ctttgctgtt attggtctgc gacagattgc cctggctgtg
    33961 taagcgttca gtggcgaccg ggcgtacccc cgtgccgatt tgtaatccgg agggtaaggt
    34021 ggtttgttcg gaagactgtg cccccggctg gcgaatggtt tgataaagca gatcttcaaa
    34081 caccgcgcgc tgacgcttaa aaccgttagt actgacgttt gccaggttgt tggcaatgac
    34141 gtccatattg gtttgctggg cgtcaaggcc cgttttggcg atccataatg aactgatcat
    34201 ataatgtcct gtagtggatt gctgacaagc agagcagaag cacgcgacag tgtgaatttc
    34261 ccctcaccct aaccctctcc ccaaaggggc gagggaatag tccgcgcgct tttattgcaa
    34321 tgtcatcaat cgttcaatta actcatcgac agcagttggt tggcacggcc tgcgttatca
    34381 tcgacgctgc tgatcacctt catctgcatt tcaaaacgcc gcgcgctggc aatcatgtcg
    34441 ctcattgccg caacggcatt gacgttactg ccttccagaa cccccgacat cacacgcaag
    34501 gttggatctg cctgcagtac cggccctcgc gtggcctggg tttctgcgct taaacgaaaa
    34561 atgccgtcgt caccgcgctg cacttcgctg cccgtggctt tcaccagttt cagacgccct
    34621 actggcgcaa ctgtatttgc cggatcgccc ggattgagcg ccgagattgt gccatcggca
    34681 gcaatcgtga tttccgcccc ttccggcaca gcaattggcc ccgcctcgcc tatcaccgga
    34741 tgcccctgaa ttgtcagttg cccggtggga tcaacctgaa tgctgccatt acgcgtatac
    34801 ccttcgctgc cgtcagcggt ctgcacggcc agccatcctg ctgcaacgca acgtccagcg
    34861 ggcgagaggt gtaatccatc ttgccgggcg tcatgtctgc acccggtgtt gacgccgtta
    34921 ccagcgtgcg cgtgggcagt gaaagccctt ccaccggcac tgcgcgcaag gcgtttaact
    34981 gcgcgcgaaa accgggcgta gaggcattgg ccagattact ggcggttacc gcctgttgat
    35041 tcagcgtctg gctggctgct cccatcgcag tataaattgc gtgatccatt gagctatccc
    35101 gtcagcgatt agcgtaagtt aacccgcgtg ttgaggatct ggtcctgggt tttgatggtc
    35161 tgggcgttag acttatagtt acgctgggca acgatcatat tgaccagttc tttactgaga
    35221 tcgacgttgg acgcttccag cgcaccgttg gtcagagtgc caaagtttcc cgtcccggct
    35281 gtccccaaca gcgccacgcc agaagattgc gtcgcagacc agacgttgtc gccttcggat
    35341 gccagacctt cgttgttggc aaagttcgcc agtacaatct gccccagcag ttgggtttgt
    35401 tcgttggaat tgttgccgac aaccgtaccg tcatcattga tttgataact caccagatcg
    35461 cccggtttgt agccattctg ggtggttgcc acaatgttgt tagcgccggt tgcgatatta
    35521 ttcgccatcg caccatccac taatgtgcca ttagcattaa attccagtgt tgtcgctgtc
    35581 ttcgcaatgc tgtttggatc actgctatcc tgggtgtaga cctgccagtt attatccccg
    35641 gtcttcacaa aatagacgct catgtcatga gcattaccct gactgtcgaa aacagtcacc
    35701 gaaccttttt tgttatagct atccgcattg ctggcgctga atggcgtaac agtaggaagc
    35761 ggatcactgg aattcaggtt gatctgcatc gacgccgtgg tggtagtttt cgctgccatc
    35821 agggtattcg ggatcgaaat attggtcgga ttcgcccctt gctgaatagt cggcggcgta
    35881 ccggttaccg ggtaacccgt cagctgtaaa ccttgcgtat tcaacaggtt acggttttca
    35941 tccagcttaa attgtccgtt acggctgtag aacaccgaac cgttgctgtc taccagacgg
    36001 aaaaaaccgt tctggctgat agcaacgtcc agacctcgcc cggtgttggt ggtcgtgcca
    36061 tcggtaaagt cctgagtgat accggcaact tttaccccca gtcccacttt cgaaccggca
    36121 aacatatcgg caaaagaggc cgtgcctgat ttaaagccgt aggtggcgga gttggcgata
    36181 ttgttgccaa taacatcgag gttggtggcg gcagcgttta atccgctaac cgcttgagaa
    36241 aaggccatga ctgactcctg aaatgtgaag gcttaaatta tctgccgtac ttcgtcgagg
    36301 gtggtggtgc cgtaagtgcc gagatccagc gtattaccgc tgttgccgcg gatcacaccc
    36361 tgcaccagag caaactgcag cggctgggca accagttgtg taccaccgtt actggcgcta
    36421 atcgctacat tgtaagaacc gttcggcgca gtgctgccat cagtcaacga accgtcccag
    36481 gtgaaactgt gaactccggc ggtcagttca ccaatatcaa tggtgcgcac aaccgcgcca
    36541 tttttatcgg tgatggtggc cgtaactttg tctgccgcct gttgcagctc aacaccaaac
    36601 ggcgtggtcg tggtcacagc cccttcttca ctgccggttc ccgcaagaac agttgtgccg
    36661 gggatcatca cgccgtgacc gatcaggtta ctggcctgta acgactggct gttatcaatc
    36721 tgtccggaaa tagatccgag cgtggtattg agtttttcaa tcccgctgac cgtgctgatt
    36781 tgtgccaatt gcgacgtcag ctcgttgttt tccattggat tggtcgggtc ctggtttttc
    36841 agctgcgcca ccagcaaagt cagaaaactg ctttgtaaat ctgcggcgtt gctgcccgtg
    36901 agcgaactac tgctggtggt actgacgccg gtatttgtcg gatcggtggt ggttaccgca
    36961 atggacatag ctttctcctt tattgaccga gcgtaagggt tttcagcatc atgcttttca
    37021 ccgtgttgag cacttcaaca ttggcctgat agctgcgtga cgccgacatg gtgttaacca
    37081 tctccccgac gacatcaacg ttcggcattt ttatgtagcc ctttgcatct gccagcggat
    37141 tacctggttc ataaaccagt ttgtccgggg cctgactttc ttaacatcgg caacctttac
    37201 gccgcctgtc gcagcacctg gtgcagcgtt aacctggaat accacctgtt ttgcccgata
    37261 tggctgtcca tcaggaccgg tcacgctatc agcatttgcc agattactgg ccgccacgtt
    37321 caggcgctgg gactgggcag ttaacgccga cccggcgata tcaaaaatat tcagcagtgc
    37381 catccgttaa tttccgctct gtaaaacgtt catcatgcct ttgatttgcc cgctcaacgc
    37441 gctaaggctc atctggtatt gcaggctgtt atcggcaaac tgggtgcgtt cgcgatccat
    37501 atcgacggta ttaccgtcaa gcgaaggctg gtccggaata cggtattgca gttctgcggt
    37561 aggaggcgtc agcgcctgcg ccgggatgtg ctgcgttgag gtcatcgtca gtgcaaccac
    37621 actggttgca tcccgtccac gttgcatgac ttttttaagt tcactggcaa aatcgatatc
    37681 gcgcgcctga taaccagggg tatcggcatt ggcgatgttt gctgccagca cttcctgacg
    37741 ctgggcgcgc agattgagcg cctcttgttg aaaacgtaag gcggcgtcga gcttatcgag
    37801 catatctcct ccgcaggtat caaaattctg ccatcagctt aaatgccttt acgcgcgcgt
    37861 tatcggcgga ataaacgcaa aatgggtcgc tatttatgcc gttgatggtc attgcggaca
    37921 ggtacaattc acgttgtaga aatggctggg ggcgaaaatg ctgacaataa aacgtagcgt
    37981 ggcgatcatc gcgatactgt tcagtccgtt aagtacggcg agcaatctca catcgcaatt
    38041 gcacaacttt tttagcgccc aactcgcggg ggtaagtgat gaggttcgtg tttctattcg
    38101 tacagcgccc aatctactac cgccatgcga gcagccattg ctttcgatga gcaataattc
    38161 ccgcctgtgg ggcaatgtga atgtgttggc acgctgcggt aacgacaaac gatatttaca
    38221 ggttaatgtg caggccacag gaaattatgt ggttgccgcg atgcctattg tgcggggagg
    38281 aaagctggaa gctggcaatg tcaaactgaa acgtggacgc ctggataccc tgccaccgcg
    38341 tacggtgctg gatatcaatc aacttgttga tgccgttagc ctgcgcgatc tatcacccga
    38401 tcaacctatc cagttaaccc agtttcgcca ggcatggcgg gtaaaagcgg gacaacgcgt
    38461 caatgtgatc gccagcggtg atgggtttag cgccaacgca gaaggtcagg cgctgaacaa
    38521 tgcagccgtc gcacagaatg cgcgggtgcg catggtatcg gaacaggtag tcagcggcgt
    38581 tgttgatgca gatgggaata ttcttataaa cctgtaagct gttaaagatt acccgtccct
    38641 tgccgataaa taagcaacac atgataaaag cgccctcaat gaggaataaa ccatgagtat
    38701 tgatcgcact tcgcctctga agcctgtaag caccgttcaa ccgcgcgaaa ccaccgacgc
    38761 gccggtaacg aacagccggg cggcaaaaac aaccgcctcc accagcacca gtgtgacgtt
    38821 aagcgacgcg caagcaaaac tgatgcaacc cggcagcagt gatatcaatc ttgaacgtgt
    38881 cgaagcgtta aaactggcga ttcgtaacgg tgaactaaaa atggacaccg gcaaaattgc
    38941 cgatgcgctg atcaacgaag cgcagcaaga cttgcagagt aactgatcgt atgacacgtc
    39001 ttgcagagat cctcgaccag atgtccgctg tgcttaacga tctcaaaacg gtaatggatc
    39061 aagagcagca acatctctct atggggcaga tcaacggcag ccagttgcaa tggattacag
    39121 aacaaaaaag ctcactgctg gcgacgctgg attacctcga acagttacgc aagaaagaac
    39181 ccaatacagc aaatagcgtt gatattagtc aacgctggca ggaaattact gtgaaaacgc
    39241 agcaactacg ccaaatgaat caacataacg gctggttact ggaaggacag attgagcgca
    39301 atcaacaggc gctggaaatg ttgaaaccgc atcaggaacc gacgctatat ggggcgaacg
    39361 gtcagacctc aacaccccat cgcggcagta aaaagatttc gatctgaata ttatctccgg
    39421 cctgcactgc aggccggaat gcattgttac accgtccggc gggcaaattc tttaactttg
    39481 aagcccagta ccgccagtgc agcgaagtac gcggcaatcc ccgccagcac gaccgccatt
    39541 aaacgcagta agcgccaggg catggtaccc aatgaccact ccggcatgat atgtaacata
    39601 cctaaaagca cgccagacat caccagtacc gccaccacca gacgcaacag aaacgccatc
    39661 cagccgggtt gcggagtaaa gattttctgc ttacgcaact gccagtaaag cagcgaagca
    39721 ttcagacacg ccgccagacc aatagaaagt gacagcccgg catgtttcaa cggaccaata
    39781 aacgccaggt tcatcaattg cgttaaaatc agcgtaacga tggcaatttt cactggcgtt
    39841 ttaatgtcct ggcgggaata aaagccagga gccaacactt tcactacaat caggccaatc
    39901 aaacccaccg agtaggcaat taacgcccgc tgggtcatca gcgcatcaaa cgcggtaaat
    39961 ttaccgtact ggaacagcga aacggttaac ggacctgaaa gaatgcccaa cgcaaccgca
    40021 ctcggcagtg ccaacaggaa acaaagacgc aacccccagt ccatcaaacg gttgtattca
    40081 tcatgattgc cactggcaaa acttttcgac agcgacggca gcaaaatggt gccaagcgcc
    40141 acgcccagca caccggaggg aaactccatt aagcggtcgg cgtaatacat ccaagacacc
    40201 gaaccggaag caagaaacga ggcaaaaatg gtgttgatga ttaaggagat ctggctcaca
    40261 gagacgccaa ggatcgccgg tcccatctgt ttcaccacgc gcattgctcc agcatcgtgg
    40321 aagttaatgc gcggcaggac cagcatgccg atcttcttca ggtgcggtag ctgatacacc
    40381 agctgcagaa tgccgccgac cgtaacagcc cacgccagcg ccagcaccgg tgggttaaag
    40441 tacggtgcgg caaacagcgc gaaaccaatc atgctgatgt taagcagtgt tggcgcaaac
    40501 gccggaatcg agaagcggtt ccacgtattc agaatcgctc ccaccagcga cgccagggag
    40561 atcagcaaga tatagggaaa ggtaatcttt agtagctggc tggtcagggc aaatttgtca
    40621 gctgtgtcag cgaagcctgg cgcggtcacc atgatcaccc acggtgcggc gagcatgcca
    40681 gcgaccgtca caaccgccag cgcaagtgtc agcaggccag aaacataaga gacaaagacc
    40741 cgcgtggcgt cttcaccctg cttactttta tattccgcca gaatcggtac aaatgcctgg
    40801 gaaaatgccc cttcggcaaa gatacggcgt aacaagttag gaagtttaaa agcgacgaaa
    40861 aaggcgtcgg ttgccatccc tgcgccaaag attctggcga caattgcgtc tcgtgcgaag
    40921 ccaagcacac gcgaaaacat ggtcatcgag ctgacggcgg ccagcgattt taataaattc
    40981 atcggtgttc taatccggac ccacgggcaa aacgcctgca aggcaggcgc tgaaagaggc
    41041 gaatagtcta cctagattat ggtgaattgc taccgccaga tgttacaggg tttattcact
    41101 catcgcatcg cgccagatct tgtcaacgat acgttgcgcc agcactgcct attcgccggc
    41161 ggtttgcgga actgtctgat tttacacgca ttcaatgaag tgccgcgcac agccgacaaa
    41221 tccacgttgc tcaagagtgc tctgccaacc aggaatcggt ttattcacta caccctgccc
    41281 gcgctcctca cgccattcgc gcatatccgt aatgtcgatg agcgcaccgt cagtcacggc
    41341 ctgcacggtt tcacgctgac ttccggcacg gcgatgcata caagtggtga tctgcaaagg
    41401 accggccgaa aaatggtgct cggcaaacag catttcgcca gcgtcgttag tcagtagcgt
    41461 accgccatcc agagaggctt tgccgcccga caaccacagc gcggtatcca ccacatgcag
    41521 ataatcatcc agcaacgtga aataaagatc gtgtggcccg acgctattgc tacgatgttt
    41581 atccattctt agcgaggctg cggtggcgag ttgcgttttt aactcaccgt agagtggtgc
    41641 gaaacgacgg ttaaaaccga ccatcaacgt cagttttttc cgcgccgcca gttccaccag
    41701 cctttcagca tcgcgcagat tttctgccag cggtttatcg acacagacat gtacccccgc
    41761 attgagtaac gtactgacca cgtcaaagtg gctggcggtg ctggagtgca caaaaaccgc
    41821 atcacaactg gcggcaaggc tggataacga atcggcataa ggaatgcgcc agctgtcaca
    41881 aattggcagg gctttggcgc gcgtaggcga ccaggctcct tgtaacgtcc agtcagacgc
    41941 tgccgccagc accggtaacc acgctttttg cgcaatgccg cctaatccca ctacgccgat
    42001 acgtaatttt ttcactttaa tctcccagat gggccagcaa cgaatcaaga cgttgtttca
    42061 gttctgccac ttcgatttcc agggcttcga cgcgggtctg taaatcaccg tcaaccatgt
    42121 tcgacatagc cgttaccgcc ggctgatctt caacctcacc actgaaaaga tgcatgtagc
    42181 ggctttcgcg tttacccggt tcgcgggcca gacgcaccac aaaaggacca tcttcgcgat
    42241 tcgccagttg ttccagcgtc gactccactt ccgccatatc gctgaattca tacattcgcg
    42301 cggcgcggct gcgcagttca cccggcgtct gggcaccacg caataacaac gtggtgatta
    42361 acgccacttc tgcggcgctc agtttcagat cgccaaattc tgaattacaa aaacgttgct
    42421 catatttggt gacccgatta ccaaaaccgc tcactgtgcg tagataatga cgtttgacca
    42481 gattatccag ctgttcctgc acttcggatt cactcagatt catgaccggt tcacggttcg
    42541 ttttctgatt acaggccgtg actacaccat tgactgagag cgggtattgc tccggcgtgg
    42601 tcacctgctt ttccagcaag caaccaatta cgcgagcttc cagggcagta agttgatatt
    42661 tcatgagatt ctccttagcg gccgggcgtc cagtctgggg tagttaatgc cgtcagtacg
    42721 tgatcgcgcc attgtccatc aatcaacaga tagtctttcg catagccttc tttttcaaaa
    42781 cccagtcgcg ccagtaaatc accgctgcgt ttattgtgcg gcatataatt agccataatg
    42841 cgatgaatat gttgggtgcg ctgcatataa cgaatggctg cggtcagggc ttcaaacatg
    42901 agtcctttgc cctgccattt ttgcccaatc gaataaccga gatagcaggc atgaaaagag
    42961 ccgcgaacaa cattggaaaa attggcaacg ccaataatct ctttttcgtc cgggtcgaat
    43021 aagccaaagt agaaagctga accttgttta tgaaattcgt taatcatccc tagcctggcc
    43081 tgccagcctg atggataaca gtggctttcg tcgcgcactg gctcccaggg cttgaggaaa
    43141 tggcgattct ctgcgtaata atccgccaga cgccaggcat cacgatcatg caccagacgc
    43201 acgaccagtc ggtctgtggt taagcgcact tttggcacgt tactgcgata gccaaacatt
    43261 ctatacctac tccttcccgt aacgtcttta atacgctgta agtattacta tacctgcgca
    43321 accagaccct gtgaaaagtc caaaatgcca ttttctgcat tattcacata tttgataaaa
    43381 actctctatc aaagcgacgt tttgataaaa aaatactgtc cccgaagtga tgggaaaagt
    43441 cttagcggca atgcagaata gaaagtgctc atcttttatt tcccgggagg ggaaatgtcc
    43501 cgcgtatcgc aggcgaggaa cctgggtaaa tatttcctgc tcatcgataa tatgctggtc
    43561 gtgctggggt tctttgttgt cttcccgctg atctctatcc gcttcgttga tcaaatgggc
    43621 tgggccgccg tcatggtcgg tattgctctc ggtctacgcc agtttattca gcaaggtctg
    43681 ggtattttcg gcggcgcaat tgccgaccgc tttggtgcca aaccgatgat tgttaccggt
    43741 atgctgatgc gcgccgccgg attcgccaca atgggtatcg cccacgaacc gtggctattg
    43801 tggttttcat gcctgctctc gggactcggc ggcacgttgt ttgatccgcc gcgttcggcg
    43861 ctggtggtga aactaatccg tccacaacaa cgtggtcgtt ttttctcgct gttgatgatg
    43921 caggacagtg ccagtgcggt cattggcgca ttgttgggga gctggctgtt gcaatacgac
    43981 tttcgcctgg tctgcgccac aggggccgtt ttgtttgtgc tatgtgcggc gttcaatgcg
    44041 tggttgttac cggcatggaa actctccacc gtacgcacgc ccgttcgcga aggcatgacc
    44101 cgcgtgatgc gtgacaagcg ttttgtcacc tatgtcctga cgctggcggg ttactacatg
    44161 ctggctgtac aagtgatgct gatgctgcca attatggtca acgacgtggc tggcgcgccc
    44221 tctgccgtta aatggatgta tgccattgaa gcgtgtctgt cgttaacgtt gctctaccct
    44281 atcgcccgct ggagtgaaaa gcattttcgt ctggaacacc ggttgatggc tgggctgttg
    44341 ataatgtcat taagcatgat gccggtgggc atggtcagcg gcctacaaca acttttcacc
    44401 ctgatttgtc tgttttatat cgggtcgatc attgccgagc ctgcgcgtga aaccttaagt
    44461 gcttcgctgg cggacgcaag agctcgcggc agctatatgg ggtttagccg tctgggtctg
    44521 gcgattggcg gcgctattgg ttatatcggt ggtggctggc tgtttgacct gggcaaatcg
    44581 gcgcaccagc cagagcttcc gtggatgatg ctgggaatta ttggcatctt cactttcctt
    44641 gcgctgggtt ggcagtttag tcagaaacgc gccgcgcgtc gtttgctgga acgcgacgcc
    44701 tgatttgata ctcccccctg actcctccat actgaaagtg aactgacagc caaatggagg
    44761 agtcacgtga agctatacat ttacgatcac tgcccttact gcctcaaagc ccgcatgatt
    44821 ttcggcctga aaaatatccc cgtcgaatta catgttctgc tcaacgacga cgcagaaaca
    44881 cccacccgga tggtcggtca aaaacaggtt cccattctgc aaaaagatga cagccgctat
    44941 atgccagaaa gcatggatat cgttcactat gtcgataaac tcgacggcaa accgttactg
    45001 accggcaaac gttcccctgc cattgaagag tggctgcgca aggtcaatgg ctacgtcaac
    45061 aaactgctgt tgccgcgttt tgccaaatcg gcatttgatg agttttctac tcccgccgcg
    45121 cgcaaatatt tcgtcgacaa gaaagaggcc agcgcgggta attttgccga cctgctggcc
    45181 cactctgacg gtctgattaa gaatatcagc gatgatttgc gtgcgctgga caaactgatc
    45241 gtcaaaccga acgccgtgaa tggcgaactt tcggaagatg atattcagct atttccgcta
    45301 ctgcgtaatc tgacgctggt agccggaatc aactggccaa gccgcgttgc taattaccgc
    45361 gataatatgg cgaaacagac acaaatcaat ttgttatcat caatggcgat ttaatcctga
    45421 cccgccgcgg cagcctgtat tcgcgggttg ccgccgtttt cagttttttc tgctggcgag
    45481 tttatcgctg gtgacacatg ctgcgttgtg tcaggatatt gacgcagatt tcatgtcgag
    45541 gactaccatg aacaagtttt tatttgccgc tgcattgatc gtcagtggcc tgctcgttgg
    45601 ctgtaatcaa ctcacccaat acaccatcac cgaacaagaa attaaccagt cgcttgcgaa
    45661 acataataat ttctcaaaag atatcggttt acccggcgtg gctgacgccc atattgttct
    45721 gacaaacttg accagccaaa ttggtcgcga agagccgaat aaggttaccc taaccggaga
    45781 cgccaatctg gacatgaact ccctgttcgg tagtcagaaa gcgaccatga aactgaagct
    45841 gaaagcgctg cccgtgtttg ataaagaaaa aggtgcgatc ttcctgaaag agatggaagt
    45901 ggtcgatgcg acggtacaac cggaaaaaat gcaaacggtg atgcaaacgt tgcttcccta
    45961 tttgaaccag gcattacgca attactttaa ccagcaacct gcttacgtcc tgcgcgaaga
    46021 tggcagtcag ggcgaagcaa tggcgaaaaa actggcgaaa ggcattgaag tgaagccagg
    46081 cgaaattgtc attccgttta cggattaatg acgagggcgc attcgcgccc tttatttttc
    46141 gtgcaaagga aaacgtttcc gcttatcctt tgtgtccggc aaaaacatcc cttcagccgg
    46201 agcatagaga ttaatgactg caccatccca ggtattaaag atccgccgcc cagacgactg
    46261 gcaccttcac ctccgcgatg gcgacatgtt aaaaactgtc gtgccgtata ccagcgaaat
    46321 ttatggacgg gctatcgtaa tgcccaatct ggctccgccc gtgaccacag ttgaggctgc
    46381 cgtggcgtat cgccaacgta ttcttgacgc cgtacctgcc gggcacgatt tcaccccatt
    46441 gatgacctgt tatttaacag attcgctgga tcctaatgag ctggagcgcg gatttaatga
    46501 agccgtgttc accgctgcaa aactttaccc ggcaaacgca accactaact ccagccacgg
    46561 cgtgacgtca attgacgcaa tcatgccggt acttgagcgc atggaaaaaa tcggtatgcc
    46621 gctactggtg catggtgaag tgacacatgc agatatcgac atttttgatc gtgaagcgcg
    46681 ctttatagaa agcgtgatgg aacctctgcg ccagcgcctg actgcgctga aagtcgtttt
    46741 tgagcacatc accaccaaag atgctgccga ctatgtccgt gacggaaatg aacggctggc
    46801 tgccaccatc actccgcagc atctgatgtt taaccgcaac catatgctgg ttggtggcgt
    46861 gcgtccgcac ctgtattgtt tacccatcct caaacgcaat attcaccaac aggcattgcg
    46921 tgaactggtc gccagcggtt ttaatcgagt attcctcggt acggattctg cgccacatgc
    46981 acgtcatcgc aaagagagca gttgcggctg cgcgggctgc ttcaacgccc caaccgcgct
    47041 gggcagttac gctaccgtct ttgaagagat gaatgctctg cagcactttg aagcattctg
    47101 ttctgtaaac ggcccgcagt tctatggctt gccggtcaac gacacattca tcgaactggt
    47161 acgtgaagag caacaggttg ctgaaagcat cgcactgact gatgacaccc tggtgccatt
    47221 cctcgccggg gaaacggtac gctggtccgt taaacaataa aaatcgtagc ttcctgttgt
    47281 cattaggtta ttttacctgt ataaataacc agtatattca acagggggct attatgcgaa
    47341 ttgaagtcac catagcgaaa acttctccat tgccagctgg ggctattgac gccctggctg
    47401 gcgaactttc ccgccgtatt cagtatgcgt ttcctgataa tgaaggccac gtatcggtac
    47461 gttatgccgc agcgaataat ttatcggtta ttggcgcaac aaaagaagat aaacagcgca
    47521 ttagcgaaat tctccaggaa acgtgggaaa gcgccgatga ctggtttgtc agcgaataat
    47581 atgcagtgat ttttgttttt gccggatcgc cccggctttt ttattttact tacgaatttt
    47641 gagtacaaaa tttctacact tcctcaaaat aattgcacaa tatgctaaaa aaatatgaat
    47701 gacatgctgt tttattgttc atataaatct taaaagcaga aatttatctg ttcccccctt
    47761 taaaaattcg gtgaataccc ttacttattg gtatactgaa gttgcttcag ataaaaacgc
    47821 attgaacctc gaataacgtt gtctagtaac acgaattagg gggccatgat ggaaaaaaat
    47881 aatgaagtca ttcagactca tccgctcgta gggtgggaca tcagcaccgt tgatagctat
    47941 gatgcgctga tgttgcgttt gcactaccag accccaaata agtccgagca ggaagggact
    48001 gaagttggtc agacgctctg gttaaccact gatgttgcca gacaatttat ttcgatatta
    48061 gaagcaggaa tcgccaaaat tgaatccggt gatttcccgg taaacgagta tcggcgtcat
    48121 taacatgaca tgaccatcca cccaaaaggc acctcaccgg tgcctttacc atttctgggt
    48181 tgtatatctg cattcagtta attactctgc ttaaaccatg agagcagaga gaaaaaaatg
    48241 aaatacgatc tcatcattat tggcagcggt tccgtaggcg ctgccgccgg gtattatgca
    48301 acccgcgccg gtttaaacgt gctaatgacc gacgcccata tgccaccgca tcaacacggc
    48361 agccaccacg gcgatacgcg attaattcgc catgcttatg gtgaaggcga aaagtatgtc
    48421 ccgttggtcc tccgcgcgca aacgctgtgg gatgaacttt cccgccacaa cgaagatgat
    48481 cccatttttg tacgctctgg tgtcattaac ctcggcccgg ctgactccgc atttctcgcc
    48541 aacgtcgccc acagcgccga acagtggcaa ctcaacgttg aaaagctcga tgcgcaaggg
    48601 attatggccc gctggccaga aatacgcgtc ccggacaact acatcggctt atttgagact
    48661 gattccggtt ttttgcgcag cgaactggcg attaaaacct ggatccaact ggcgaaggaa
    48721 gcgggctgtg cgcaactgtt caactgcccg gtcaccgcaa ttcgtcatga cgatgatggc
    48781 gtaactattg aaacggttga cggtgagtat caggcgaaaa aagcgattgt ctgcgcggga
    48841 acatgggtaa aagacctgct cccggagctg cctgtccagc ccgtacgtaa agtatttgcc
    48901 tggtatcagg ccgatggccg ctatagcgtg aagaataaat tcccggcgtt taccggtgaa
    48961 ctgcccaatg gcgatcaata ttatggtttt ccggcagaaa acgacgcgtt gaagattggc
    49021 aaacataacg gaggccaggt tatccattca gcggatgaac gtgttccgtt tgcggaagtg
    49081 gccagtgatg ggtcagaagc cttcccgttc ttgcgcaatg tattgccggg tatcggttgc
    49141 tgcctgtacg gcgctgcctg cacctatgat aattcgcctg acgaagattt tattatcgat
    49201 accctacccg gccacgataa tacactgctc attaccggcc tgagtgggca cggttttaaa
    49261 tttgcgtcag ttttagggga aatagctgcc gattttgcgc aagaccaaaa aagcgatttt
    49321 gatttgacgc cattcaggct ttcccgcttc caataatcat cattacggcc tccagaatga
    49381 ggccgttttt acaggagtat ttaatgcgtc ggctgttgca ctatctcatc aataatattc
    49441 gcgagcatct gatgctttat ctttttctct ggggattgtt ggcaattatg gatctgatct
    49501 atgtgtttta tttttagttt tccgcactga cataaactga caaatattta atctcccttt
    49561 catttagcgt gaaaaattga atgagattat tcctgcttct acagcactca ccagacatct
    49621 ctgatgctga acccactaaa cttatgaaga ttctttcatt attaattcaa aattattgaa
    49681 ataaaaataa atatcattca atttgttatt tttgtgttgc aattcattct ccgacacgcc
    49741 attttctttc tctcgcaaat tcgtattgga tgcacttatg tcattcacaa atacccctga
    49801 acgctatggt gttatatctg cggccttcca ttggctaagc gcaattattg tctacggcat
    49861 gtttgcttta ggtctgtgga tggtcacgct cagttattac gatggctggt atcacaaagc
    49921 acccgaactg cataaaagca tcggtatttt gttaatgatg gggctggtta ttcgcgttct
    49981 gtggcgtgtc atatctccac cgccgggtcc gctgccgagt tattcgccca tgactcgtct
    50041 tgccgcaaaa gctggacatc tcgcattgtg cctattgctg ttcgccatcg gcatcagcgg
    50101 ctatctgatc tcaactgccg atggtaaacc gatcagcgtt tttggctggt ttgacgtccc
    50161 cgcgaccctt gccgacgctg gcgcacaggc agactttgcc ggtgccctgc atttttggct
    50221 cgcgtggagc gtcgtagtac tgtctgttat gcacggattt atggccctga agcatcattt
    50281 catcgataaa gacgacactc tgaagcgcat gctgggaaag tcgtcatctg actatggagt
    50341 ataaaaaatg aaaaaaagcc tgcttggttt aaccttcgcg tccctgatgt tctctgccgg
    50401 ttcagcggtt gccgccgatt acaaaattga caaagaaggt cagcacgcct ttgttaactt
    50461 ccgcatacag catcttggct atagctggtt atatggcacc tttaaagatt tcgacggtac
    50521 ttttaccttt gatgaaaaaa atccggctgc cgataaagtg aatgtgacaa ttaacaccac
    50581 cagcgtcgat actaaccacg ccgaacgcga taaacatctt cgcagtgcgg attttctcaa
    50641 taccacaaaa tatccacagg caacattcac ctccaccagc gtgaagaaag acggtgacga
    50701 actggatatt accggcgatc tgacgctgaa tggcgtaacc aaacccgtca cgctggaagc
    50761 gaaattaatt ggtcagggcg acgacccatg gggcggtaaa cgtgcaggct ttgaggccga
    50821 aggcaaaatt aagctcaaag acttcaatat caagacagat ttaggtccag cttctcagga
    50881 agtggatctg attatttcag tggaaggcgt acagcagaag taagagtgaa atgttgccgg
    50941 atggcacgca tccggcaatg atatttattc tgttggatca ggaataccca gtgttgtatt
    51001 cagacgtcca cgagacttat taaagatctt attgccattt tcacgtcctg cccgacggcg
    51061 tcgctgttct tctggcggta acgcgctttc ttcgcagcaa atctcactac aacaaccttt
    51121 gtatttttcc gcgcatactg gacactgaat aaacagcagg tggcagccat catttttaca
    51181 gttggtatgg ctgtcgcacg gcgcgccgca ctggtggcaa tgcgcgataa tctcatccga
    51241 tatacgttcg cccatccgct cgtcaaaaac aaaatttttg ccaataaaac gcaccggcaa
    51301 gccctgctcg cgcgccttac gggcgtattc aataattccg ccctcgatat gccagacttt
    51361 gttgaatccg ttatgtttca tccaggcact ggccttttca caacgaatgc cgccggtgca
    51421 gtacatgacg atttttttat ctttatgtgc ctgcatcatc tcgactgctt ttggcagctg
    51481 ctcacggaag gtatctgccg gaatttcgag cgcgttttca aagtgcccca cttcatactc
    51541 atagtggtta cgcatgtcga taaatagtgc atcgggatcg tcaagcatgg cgttcacttc
    51601 cgccgcttgc agatactcac caacattgct ggcatcaaag tgaggatcgt caataccgtc
    51661 ggcaacaatg cgatcgcgta ccttcatgcg cagtacccag aaggatttcc cgtcatcatc
    51721 caacgcgata ttcaggcgta aaccctctaa agccgggtcg aaggcataga gctgcgcgcg
    51781 aaatgtttca acattgctcg caggtacgct gatttgcgcg ttaatgccct catgcgccag
    51841 atacactcgc ccaaaaacat tcagcgcggt aaacagctga tataaagcgt cacgggtcgc
    51901 cttaggatcg gcgatgtgga aatacttgta aaacgaaatg gtggttcgcg gttcgctctc
    51961 agccaacatt ttggctttta acgcgtcgtt ggaaatgcgg ttgtgtaaca ctggcatggt
    52021 gtacggttcc tgcgagatgg gaaagtaaaa atccgcggca tgatatagca attatcgata
    52081 attaacatcc acacatttta cgctacattt gcgcattaaa aattatttgt tatttacaag
    52141 cgcggcaatt tcgcccagtc ttcagccaca aattttggtt gcgggcgaaa aaatgcgaca
    52201 atacatacaa ttgcccgtat aggttgaaaa acaggattga tatgacgaat ctacccaagt
    52261 tctccaccgc actgcttcat ccgcgttatt ggttaacctg gttgggtatt ggcgtacttt
    52321 ggttggtcgt gcaattgccc tacccgatta tctaccgcct cggttgtgga ttaggaaaac
    52381 tggcgttacg ttttatgaaa cgacgcgcaa aaattgtgca tcgcaacctg gaactgtgct
    52441 tcccggaaat gagcgaacaa gaacgccgta aaatggtggt gaagaatttc gaatccgttg
    52501 gcatgggcct gatggaaacc ggtatggcgt ggttctggcc ggaccgccga atcgcccgct
    52561 ggacggaagt gatcggcatg gaacacattc gtgacgtgca ggcgcaaaaa cgcggcatcc
    52621 tgttagttgg catccatttt ctgacactgg agctgggcgc gcggcagttt ggtatgcagg
    52681 aaccggggat tggcgtttac cgcccgaacg ataatccact gattgactgg ctacaaacct
    52741 ggggccgttt gcgctcaaat aaatcgatgc tcgaccgcaa agatttaaaa ggcatgatta
    52801 aagccctgaa aaaaggcgaa gtggtctggt acgcaccaga tcatgattac ggcccgcgct
    52861 caagcgtttt cgtcccgtta tttgccgttg agcaggctgc gaccacgacc ggaacctgga
    52921 tgctggcacg gatgtccggc gcatgtctgg tgcccttcgt tccacgccgt aagccagatg
    52981 gcaaagggta tcaattgatt atgctgccac cagagtgttc tccgccactg gatgatgccg
    53041 aaactaccgc cgcgtggatg aacaaagtgg tcgaaaaatg catcatgatg gcaccagagc
    53101 agtatatgtg gttacaccgt cgctttaaaa cacgcccgga aggcgttcct tcacgctatt
    53161 aaatctccca tgccggatgc ttcagaatgg catccggcat taccacagca aatccccctg
    53221 atttagcgat aaaagctctc tggattgcgc cccctggaag tcgggcgcat aattagtgtg
    53281 tttatctttt cttcttatgt tcaccgcgcc tggcgcacca acagcggatt gctatgtcac
    53341 cctgtgaaaa tgacacccct ataaactgga aacgaaacct gatcgtcgcc tggctaggct
    53401 gttttcttac cggggccgcc ttcagtctgg taatgccctt cttacccctc tacgttgagc
    53461 agcttggtgt taccggccac tccgccctga atatgtggtc cggtattgtc ttcagtataa
    53521 tgggttccta aacacatcat tttaggtatt gaatatgaga cgggcaatca gctacatcag
    53581 attttcaagt gaacgacaac tgaaagggga ctctgtacgc cgacaatcta agctggttac
    53641 tgactggctt gataagaacc ctgagtttta cttggattca tccttgagct ttaaagattt
    53701 ggggaaaagt gcttttagtg gtaagcacct gaaaggtggt ctaggtgact ttttaaccgc
    53761 tatcgagaaa gggcttgtta aagctggtga tacgctacta atcgagagtt tagaccgtct
    53821 gtcacgtcag gatattgata ttgcttctga gctattacgc cgtatcttaa gggctggtgt
    53881 agatgtagtc acactgagcg atggagaaca ttacacacga gaaagtttaa aagacccgtt
    53941 agccctgatt aaaagcattc tgataatgca gcgggcgcat gaggaaagtt tgagaaagtc
    54001 tgagcgtgtc caagctgcat ggaaccgtaa aaaagaactc atctccgaag gtatcaaggt
    54061 atctaggcgt tgtcctgcat ggttaaggct gaatgatgac cgtcgaacat tcacaatcat
    54121 tcctgataaa gtggaagttg ttaaacgagc atttgacctg agattacagg gtttaagctt
    54181 ttgggctatc accagaactt taaacgatga aggtcacttg tcattgaatc aatacacacc
    54241 caaacaaaag gggtggtctg atactgctgt aaagaaactg ctcagaaatc gtgctgtgat
    54301 tggatgtttc acacctgctg gacgtgaaga agttcagggt tactaccccg caataatcag
    54361 tgagtcacta ttctatcgag tacagcaact gaacacaggg caatatggta gggcttctgt
    54421 atcaagtaat ccgctatcag tcaacttgtt caggggtatc atcaa
//
LOCUS       DAZPCS010000013        53210 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZPCS010000013 DAZPCS010000000
VERSION     DAZPCS010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53210
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.013"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(11..322)
                     /locus_tag="ACHUQ7_000713"
     CDS             complement(11..322)
                     /locus_tag="ACHUQ7_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFR1134252.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            485..581
                     /gene="ffs"
                     /locus_tag="ACHUQ7_000714"
     ncRNA           485..581
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHUQ7_000714"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            701..1054
                     /locus_tag="ACHUQ7_000715"
     CDS             701..1054
                     /locus_tag="ACHUQ7_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFR1134253.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="ACHUQ7_000716"
     CDS             complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="ACHUQ7_000716"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HFR1134254.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPELGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FLEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(2810..3280)
                     /locus_tag="ACHUQ7_000717"
     CDS             complement(2810..3280)
                     /locus_tag="ACHUQ7_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFR1134255.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(3396..3947)
                     /gene="maa"
                     /locus_tag="ACHUQ7_000718"
                     /pseudo
     CDS             complement(3396..3947)
                     /gene="maa"
                     /locus_tag="ACHUQ7_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
     gene            complement(4119..4337)
                     /locus_tag="ACHUQ7_000719"
     CDS             complement(4119..4337)
                     /locus_tag="ACHUQ7_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFR1134256.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="ACHUQ7_000720"
     CDS             complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="ACHUQ7_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFR1134257.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(5282..8431)
                     /gene="acrB"
                     /locus_tag="ACHUQ7_000721"
     CDS             complement(5282..8431)
                     /gene="acrB"
                     /locus_tag="ACHUQ7_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFR1134258.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWLNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSTPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(8454..9647)
                     /gene="acrA"
                     /locus_tag="ACHUQ7_000722"
     CDS             complement(8454..9647)
                     /gene="acrA"
                     /locus_tag="ACHUQ7_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFR1134259.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATVLATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            9789..10436
                     /gene="acrR"
                     /locus_tag="ACHUQ7_000723"
     CDS             9789..10436
                     /gene="acrR"
                     /locus_tag="ACHUQ7_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFR1134260.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            10564..13926
                     /gene="mscK"
                     /locus_tag="ACHUQ7_000724"
     CDS             10564..13926
                     /gene="mscK"
                     /locus_tag="ACHUQ7_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFR1134261.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(14138..14299)
                     /gene="rsmS"
                     /locus_tag="ACHUQ7_000725"
     CDS             complement(14138..14299)
                     /gene="rsmS"
                     /locus_tag="ACHUQ7_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFR1134262.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(14313..14840)
                     /gene="priC"
                     /locus_tag="ACHUQ7_000726"
     CDS             complement(14313..14840)
                     /gene="priC"
                     /locus_tag="ACHUQ7_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFR1134263.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQSCLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKISRWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            14910..15287
                     /locus_tag="ACHUQ7_000727"
     CDS             14910..15287
                     /locus_tag="ACHUQ7_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFR1134264.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            15440..15991
                     /gene="apt"
                     /locus_tag="ACHUQ7_000728"
     CDS             15440..15991
                     /gene="apt"
                     /locus_tag="ACHUQ7_000728"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFR1134265.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            16120..18045
                     /gene="dnaX"
                     /locus_tag="ACHUQ7_000729"
     CDS             16120..18045
                     /gene="dnaX"
                     /locus_tag="ACHUQ7_000729"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFR1134266.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     VKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    17378..17442
                     /gene="dnaX"
                     /locus_tag="ACHUQ7_000729"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            18098..18427
                     /locus_tag="ACHUQ7_000730"
     CDS             18098..18427
                     /locus_tag="ACHUQ7_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFR1134267.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            18427..19032
                     /gene="recR"
                     /locus_tag="ACHUQ7_000731"
     CDS             18427..19032
                     /gene="recR"
                     /locus_tag="ACHUQ7_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFR1134268.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            19142..21016
                     /gene="htpG"
                     /locus_tag="ACHUQ7_000732"
     CDS             19142..21016
                     /gene="htpG"
                     /locus_tag="ACHUQ7_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFR1134269.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            21197..21841
                     /gene="adk"
                     /locus_tag="ACHUQ7_000733"
     CDS             21197..21841
                     /gene="adk"
                     /locus_tag="ACHUQ7_000733"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFR1134270.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKQVAEVRADLEKILG"
     gene            21973..22935
                     /gene="hemH"
                     /locus_tag="ACHUQ7_000734"
     CDS             21973..22935
                     /gene="hemH"
                     /locus_tag="ACHUQ7_000734"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFR1134271.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESSVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMVPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(22932..23891)
                     /gene="aes"
                     /locus_tag="ACHUQ7_000735"
                     /pseudo
     CDS             complement(22932..23891)
                     /gene="aes"
                     /locus_tag="ACHUQ7_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
     gene            24043..25347
                     /gene="gsk"
                     /locus_tag="ACHUQ7_000736"
     CDS             24043..25347
                     /gene="gsk"
                     /locus_tag="ACHUQ7_000736"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFR1134272.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEVLYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVELVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSQEESYWDR"
     gene            complement(25480..27156)
                     /gene="ybaL"
                     /locus_tag="ACHUQ7_000737"
     CDS             complement(25480..27156)
                     /gene="ybaL"
                     /locus_tag="ACHUQ7_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFR1134273.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(27394..28614)
                     /gene="fsr"
                     /locus_tag="ACHUQ7_000738"
     CDS             complement(27394..28614)
                     /gene="fsr"
                     /locus_tag="ACHUQ7_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFR1134274.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIEPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            28832..30484
                     /gene="ushA"
                     /locus_tag="ACHUQ7_000739"
     CDS             28832..30484
                     /gene="ushA"
                     /locus_tag="ACHUQ7_000739"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFR1134275.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNLEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGKTNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(30521..31000)
                     /gene="ybaK"
                     /locus_tag="ACHUQ7_000740"
     CDS             complement(30521..31000)
                     /gene="ybaK"
                     /locus_tag="ACHUQ7_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFR1134276.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(31204..31998)
                     /locus_tag="ACHUQ7_000741"
     CDS             complement(31204..31998)
                     /locus_tag="ACHUQ7_000741"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFR1134277.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            32136..32477
                     /locus_tag="ACHUQ7_000742"
     CDS             32136..32477
                     /locus_tag="ACHUQ7_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFR1134278.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(32692..35196)
                     /gene="copA"
                     /locus_tag="ACHUQ7_000743"
     CDS             complement(32692..35196)
                     /gene="copA"
                     /locus_tag="ACHUQ7_000743"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFR1134279.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMIVTADNRSL
                     WLVIGLITLAVMVFASGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAIFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            35458..36390
                     /gene="glsA"
                     /locus_tag="ACHUQ7_000744"
     CDS             35458..36390
                     /gene="glsA"
                     /locus_tag="ACHUQ7_000744"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFR1134280.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            36393..37685
                     /locus_tag="ACHUQ7_000745"
     CDS             36393..37685
                     /locus_tag="ACHUQ7_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFR1134281.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            37716..37813
                     /locus_tag="ACHUQ7_000746"
                     /pseudo
     CDS             37716..37813
                     /locus_tag="ACHUQ7_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            37810..38217
                     /gene="cueR"
                     /locus_tag="ACHUQ7_000747"
     CDS             37810..38217
                     /gene="cueR"
                     /locus_tag="ACHUQ7_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFR1134282.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(38218..38676)
                     /locus_tag="ACHUQ7_000748"
     CDS             complement(38218..38676)
                     /locus_tag="ACHUQ7_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFR1134283.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(38673..39590)
                     /locus_tag="ACHUQ7_000749"
     CDS             complement(38673..39590)
                     /locus_tag="ACHUQ7_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFR1134284.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            39736..40413
                     /gene="fetA"
                     /locus_tag="ACHUQ7_000750"
     CDS             39736..40413
                     /gene="fetA"
                     /locus_tag="ACHUQ7_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFR1134285.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            40400..41179
                     /gene="fetB"
                     /locus_tag="ACHUQ7_000751"
     CDS             40400..41179
                     /gene="fetB"
                     /locus_tag="ACHUQ7_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFR1134286.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGAIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(41242..42096)
                     /gene="cnoX"
                     /locus_tag="ACHUQ7_000752"
     CDS             complement(41242..42096)
                     /gene="cnoX"
                     /locus_tag="ACHUQ7_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFR1134287.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(42157..42966)
                     /gene="ybbO"
                     /locus_tag="ACHUQ7_000753"
     CDS             complement(42157..42966)
                     /gene="ybbO"
                     /locus_tag="ACHUQ7_000753"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFR1134288.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            complement(42956..43578)
                     /gene="tesA"
                     /locus_tag="ACHUQ7_000754"
                     /pseudo
     CDS             complement(42956..43578)
                     /gene="tesA"
                     /locus_tag="ACHUQ7_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
     gene            43549..44235
                     /gene="ybbA"
                     /locus_tag="ACHUQ7_000755"
     CDS             43549..44235
                     /gene="ybbA"
                     /locus_tag="ACHUQ7_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFR1134289.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQLFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            44232..46647
                     /gene="ybbP"
                     /locus_tag="ACHUQ7_000756"
                     /pseudo
     CDS             44232..46647
                     /gene="ybbP"
                     /locus_tag="ACHUQ7_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
     gene            complement(46865..47020)
                     /gene="dinQ"
                     /locus_tag="ACHUQ7_000757"
     CDS             complement(46865..47020)
                     /gene="dinQ"
                     /locus_tag="ACHUQ7_000757"
                     /inference="COORDINATES: protein motif:HMM:NF041472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFR1134290.1"
                     /translation="MKKIIVIGNVRVSCDCLSPLREQRMKTFLDKVLIVLKALIALLE
                     LIRQFIE"
     gene            complement(47360..48454)
                     /gene="mnmH"
                     /locus_tag="ACHUQ7_000758"
     CDS             complement(47360..48454)
                     /gene="mnmH"
                     /locus_tag="ACHUQ7_000758"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFR1134291.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMD
                     NDERASVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(48523..49449)
                     /gene="allS"
                     /locus_tag="ACHUQ7_000759"
     CDS             complement(48523..49449)
                     /gene="allS"
                     /locus_tag="ACHUQ7_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HFR1134292.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSAGTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSCKAVEDIVTLFTQHRPEISGFLEIFGTPRS"
     gene            49679..50161
                     /gene="allA"
                     /locus_tag="ACHUQ7_000760"
     CDS             49679..50161
                     /gene="allA"
                     /locus_tag="ACHUQ7_000760"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HFR1134293.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGIQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            50239..51054
                     /gene="allR"
                     /locus_tag="ACHUQ7_000761"
     CDS             50239..51054
                     /gene="allR"
                     /locus_tag="ACHUQ7_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HFR1134294.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGRVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            51144..52925
                     /gene="gcl"
                     /locus_tag="ACHUQ7_000762"
     CDS             51144..52925
                     /gene="gcl"
                     /locus_tag="ACHUQ7_000762"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HFR1134295.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGGCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            52938..>53210
                     /locus_tag="ACHUQ7_000763"
     CDS             52938..>53210
                     /locus_tag="ACHUQ7_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFR1134296.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAI"
BASE COUNT        12830 a        13688 c        14016 g        12676 t
ORIGIN      
        1 ccggagaggg ttagtagttc cagcgataac gctgcaagtc gatttgcccg cttcccgata
       61 ccatcacacc ttctgccagt aatgcctgtc gctgacgctg taaatccggc ccggttagcg
      121 aaattgtgcc atggcgatta accacccggt gccagggtaa ggtgctgcct tcagggagac
      181 gctttaacac accgcccacc tggcgcgcgg cgcggggcga tcccgccagt ttcgccacat
      241 caccgtaagt ggtgacatag ccttcgggaa tagcggcgac gatttgccag acgcgttggg
      301 gaaatgaatc ttctttttcc atcttttctt cctgaggtaa tttttcagca taatctggaa
      361 aaacgcccga gtgaagtcgc attgcgcaag aaaccagcat ctggcacgca atgggttgca
      421 attagccggg gcagcagtga taatgcgcct gcgcgttggt tttcaacgct ctcaatgggg
      481 gctctgttgg ttctcccgca atgctactct gtttaccagg tcaggtccgg aaggaagcag
      541 ccaaggcaga tgacgcgtgt gccgggatgt agctggcagg gcccccaccc atttctgcct
      601 cccaccgttt cgtcaaaaaa tcccagcatg gctaaacttt aaccacaact gacgtcgcaa
      661 gaattgtctg gctgcgcagt acgcttcgga ggtatgtctg atgaagtatg ttgatggttt
      721 tgtggttgcc gttcctgccg ataaaaagga tgcctatcgg gaaatggccg ctaaggccgc
      781 gccattgttt aaagagtttg gcgcgcttcg tattgtcgaa tgctgggcca gcgatgtgcc
      841 ggatggcaaa gtgaccgatt ttcgtatggc ggtgaaagcg gaagagaatg aagaggttgt
      901 ctttagctgg attgaatacc cttcaaaaga ggtccgcgac gcagctaatc aaaagatgat
      961 gtcggaccca cggatgaaag agttcggcga gtccatgccg tttgacggca agcgaatgat
     1021 ctatggcgga ttcgagtcaa tcatcgacga ataggtagcg tgacgggctg tgcggcgtgc
     1081 cgtcagccca aaaattcaca aatgttgctc ggcccagcgt aagaaatctt ctttcggtaa
     1141 tgccttgctg tagagccagc cctgaccgta gtgcacgcca tgctggcgta gccactcttc
     1201 ttgtttactg gtttcgattc cctccgctac cattttcagt ttcagtgttt ttgccatttc
     1261 gatgatatgc ggcgtgacat ttttatattc cagcgcatca acgaaagatt tatcgatctt
     1321 cagaatgtcg acatccagat cctgtaaata acttaaactt gaataccccg taccaaaatc
     1381 atcaagataa atttcatggc ccgcctcccg gtagcgagaa attatcgggg cgctggtttt
     1441 cggatcggca aactcgcgtt cagtgagttc aagcgcgatc tgtctgggat taacctgata
     1501 gtgattgatc atttcacgca gcaattgcgg gattttttcc gaggtgagca cggtggattc
     1561 aagattgatc gaaatatgct gctgtggatg ctgacgcaag cagtcgccca tatcttcaaa
     1621 gacgcttctt ataatcagta gcgtcaatgg ctcagaaagg cccgtttgct gtgccagcgg
     1681 aataaaacta tctggtgaca accaactacc gtctgtctgc ggccagcgcg ccagtgcctc
     1741 agcaccgaca attttgccat tggctaagga gacaatcggc tgatagtgca cgcaaatatc
     1801 acgattttcg atagcatcct gcagccgatg atgcggtgat tggatacggc gcaaaatacg
     1861 cagcacaaac atcgctgcca gcaggccaat caccaacccg agcggtaacc aaataaagac
     1921 ttgccgatgc cagcctttct gcaacatttt cgttgaagcc caggtgatga tcgaaatatt
     1981 catctccagg aagggcagta tatcgtaaat gatcccatta ttttcgatat gctcaccggg
     2041 tgttttttgt agcctggtaa taattccctg agcaatttca tcgctgctgg ttatgacaac
     2101 gttatgggca ttgccaataa tggcggcatc aatttgccat gaactatagg gaatgacatc
     2161 aataaaggaa gcggggtcga tcatgacgac ataatgtgcc gttcccatgg cgaccatgta
     2221 acggataatg cctaaatcgt tatgcgatgt taaccagaca cgataaccat ctttcgaaat
     2281 tttacctagc tcgggaaagg tatcgggcgg actttcatgc tccagagacg aacactgggg
     2341 aacgttgtta tcgatataaa ccacttcctg aatatagcga taactgtaag atacccgacg
     2401 catttccatg agatgggctt cgctacaggc agcgccttgc catctttcca gctcctgcag
     2461 cgcatctttc ccttgtgtcg ccaccttatt ggctcgaata gcgacgcggg aggaataggt
     2521 atccagctct tcaataaacg atgtttctac ctgctgatgg gccagccaga tgcttaagcc
     2581 gacaggcagc aatactgaaa gaatcagtac tcccgaaata aggccgacca gatgtcgtgt
     2641 tctcaccagc atatccttat taacacagtg tagggaaagt atatctgatt gtggaaaaag
     2701 gctggcagga gataaggagg tagagctggt atttcatgaa gtgatgggtg gaaacgatca
     2761 ttgtcatttg accaggcgca gagctttacg cctggtatgt aaccctaact taagatgttg
     2821 attcggcgcg agcgagggta aaaatatcgt aaaaagacag ttcaccttta cggacgatca
     2881 ttttttgcag ttgttcctta tgaacatccg cgacacgcgg agagttcaag atggcttcaa
     2941 ccagcgcggc aggtacaaag cgcgtgttgt accattcgcc gttatagcaa acgcggaaat
     3001 ccagcacgtc gatatcttca tagcggtagc gtgggtagac atcgaaaaag aagaaaccat
     3061 taagcaggat aaataataca accagtagcc agacagagcc tgacagcgtt tcggactgca
     3121 acatcaccgc cagggtggca aaaaaagcaa cgtacatacc gataaacagc cccggatgtt
     3181 tacggataaa actgatacta aagcggggtt tgttgtcgcg tttttcgcgg gtattcagag
     3241 actcaatcgt ttcggtcagc aggcgttgta tttcggtcat cgtttgcctt cgtggtatct
     3301 gcaactttcc agggaacacc ctattttaac gtgggaatag tagaggggaa gtaacagatc
     3361 taccacaatt ttgcataaca gttgcgaaaa accgattaca attttttaat tattctggct
     3421 ggattaccgc ccacgacaac gttgtccggg acaccttttg tgacaactgc acctgaagct
     3481 accacgacgt tatcaccaat ggtcacacca gggttaatga ccgcgcgtcc gccaatccag
     3541 acgttattac cgatggtgac gggtttcccc agttcagcac cgctattacg tgctacaggg
     3601 tcgatgggat gtgttgccgt gtagatatga acgcctggtg ccaacataca gttatcaccg
     3661 atgcgaatag ggcagacatc aagcatcacg caatcgaagt tggcgaaaaa attattgccg
     3721 agaaaaatgt tatagccata atcacagcga aacgttggct caatataagc ctctgtcacc
     3781 tgaccgaata aattagcgag aatttactgg cgtaatgtgt gctcttccgc cagggaatga
     3841 ttgtatcggt gaataagctg acgagcgcgc aggcgatcgc gagataacgt ctcatctgcc
     3901 gagcgataca actcaccagc aatcatcttt tctttttctg tgctcattac tgacctccgg
     3961 ttgctgaaaa tgcggcaaca cagtaagacg aaaagtcagt gaagagaacg ttctcattac
     4021 atctgtgtga taaagatcac atagggtatg tcttcatggc gaaaaagtat aaaattctta
     4081 ataaacagcc ggttatagca ccgaaagcga attatttatt agcgaataaa tttccatact
     4141 gaggaaggga tcttgtcgta cagtttattc atggtcaatt cggcgaggcg gtgatctgcg
     4201 gctgagtaaa ataccgccag ttcattatct gataattcgt atttattttt ctcgataaca
     4261 cgctccagcg tgtcaattgt ctggcaacga cgtaaacgca ttaaataatc ggtttttgtt
     4321 aaaggttttt cggacatact tctacccatg gttgtaataa ttctaacaag ataaactcgc
     4381 aggattctct ttcgtcgcgt tgacaaaaca acggaatagt cgattacctg acttccgcca
     4441 tttctgaaga gcctgcatat taataccata actgctgaac aacataaagg tgtcatccag
     4501 atattcgtcg atctgctcaa tgagcttatt gtcttcatta tacttaattt tgtaattaag
     4561 tgcgaaggtc gcaatatgct caatcagttc attcaactgg aggttgatcg ccgaggttgg
     4621 gtcgttaacc caaccatgat tgctttcttc aaggtttgca agacagtcat gatacagggt
     4681 ttcacagaga aacttaagct gtgcgatatc atgtcttttg ggtgagtatt catccataac
     4741 gcgtcccctt cttagtggtt gaactaacgg acacctttcg ggatggaaaa aacttactga
     4801 cctagacttg ccctctcgtt tgttagctta actataagcc actctttgca ggttttcatc
     4861 gcattattac gaaacattac aattaggaag gaatcgggac aggactcact ttttcatact
     4921 agcactaacc cgcagcaggt aaaagcagtt tgctcgaact gttcataaca tttcaggatt
     4981 atcgccagat gaaattggtg acactaatac caggattgct ctgaatatga cgtaataacc
     5041 gaggaatgaa taaagaatta ccgcaaataa ttaagaatag cctctaaatg attatggatt
     5101 attaaatcta ttagcgctac ttaatataaa ttaactaaca atcagtattt tttatgaatt
     5161 ttatacgtgg ttaatactgg ttttcgtatg agatcctgag ttggtggttc aattactcct
     5221 taatgttcgt aggttatgca taaaaaggcc gcttacgcgg ccttagtgat tacatgttgt
     5281 atcaatgatg atcgacagta tggctgtgct cgatatcttc attcttgcgg ctaaagcggc
     5341 ggcgaaccac cacaaagaat accggaacga agaagattgc cagtaccgtt gcggtcacca
     5401 tcccgcccat tacaccggta cctactgcgt tctgcgcgcc ggaaccagca ccagtactga
     5461 taaccagcgg cataacgccg aggataaacg ccagcgaggt catcaggatc ggacgtaaac
     5521 gcatccgcac cgcatcaagc gtcgcttcaa tcagaccttt accttcttta tccatcaagt
     5581 ctttggcgaa ttcgacgata agtatcgcgt tcttcgccga caacccaatg gttgtgagca
     5641 gtcctacttg gaagtaaacg tcattggtca ggccacggaa ggtggcagcc agcaacgcac
     5701 cgataacccc cagcggaacg accagcataa cggagaacgg agtcgaccag ctctcgtaca
     5761 gcgccgccag acacaggaat acgacaatca acgaaatcgc gtacagtgaa ggtgcctggt
     5821 tgccggagag acgttcctga taggacatcc ccgtccagtc atagccaaca ccggtaggca
     5881 gtttgctcgc cagttgttcc atcagctcca ttgcttcacc ggtactttta cccggtgccg
     5941 cctggcctaa gatttccatg gatggcaggc cgttgtaacg ttccagacgc ggcgaaccgt
     6001 actcccaacg agaagaggag aacgccgaga atggcaccat ctgaccatca gcagcacgaa
     6061 cataccagtc gccgatatca tccggcagca tacggtattt cgcttctgac atgacgtaaa
     6121 ctttcttcac acgaccgcgg tcgataaagt cgttcacata gctaccgccc catgcagcgc
     6181 ccagagtggt gttaatgtcg ttgatagaaa cacccagcgc ctgcgctttt tcctggtcga
     6241 tatcaatctt aaactgcggg gtatcttcca gaccgtttgg acgtacgctg gtcagcatat
     6301 cagggtgctt cgctgcttct gcaagcagct ggttacgcgc ctgagtcagt ttttcgtgac
     6361 caaggccagc ctggtcaatc agctcaaagt caaagccggt tgcagtaccc agttccacga
     6421 ttgcgggcag gttaaaggcg aaaaccatcg catctttgat ttgcgagaaa gcgcgtgttg
     6481 cacgcatggt aatcgcttca actttgtttt cttcgcccgg acgatcggcc cagtccttca
     6541 aggaaacgaa cgcaataccg gtattctgac cacgtcccgc aaagccgaag ccgttaacgg
     6601 cgaacaccga ctcaacgttg ttcttttctt tggtcagata gtaatgcgtt acctcattga
     6661 gcactttctg cgtacgttcc tgcgttgcac ctgctggcag ctgaaccatg gtcataaata
     6721 cgccctggtc ctcatctggc aagaaggagc ttggcagacg cacgaacaga taggccatgc
     6781 cgaccacgat gatcagatac agcactaggt aacgccccgt actgcgcaga ataccgccta
     6841 cgctgtcggt gtagtggtgc gtgctcttct cgaacatgcg gttcaaccag ccgaagaagc
     6901 cttttttacc ttccccgtga tcgcctttgg caatcggttt cagcatggtg gcacaaagag
     6961 ccggagtcag gatcaacgct accagtaccg acagcgccat tgctgaaaca atggtaatag
     7021 agaactgacg atagatagca ccagtagaac cgccaaagaa ggccatcggt acgaataccg
     7081 ccgacagtac catcgcgata ccgaccagag cgccctgaat ctgccccatc gacttacggg
     7141 tagcttcttt tggcggcaaa ccttcttccg ccataacacg ctccacgttt tctaccacaa
     7201 cgatagcgtc atccaccaac aagccgatgg cgagcaccat cccgaacatt gttagcgtgt
     7261 ttatcgagaa gccaaaggcg gcaaggacgg caaaggtccc gagcaatacc accggtacgg
     7321 caatggtcgg aatcaacgtc gcgcggaagt tctgcaggaa cagatacata accaggaaca
     7381 cgaggatgat cgcttcgacc agcgttttaa ccacttcgtg aatagagatt ttcacgaacg
     7441 gcgtggtgtc gtacgggtaa acaattttca gacccgacgg gaagaacggt tccatcttcg
     7501 ccagttcagc acggattgcc gcagcggtat ccagcgcgtt tgcaccggtc gccagcttga
     7561 tccccagacc ggaagcaggt tggccgttaa actctgcgat gatgtcgtag ttctcaccac
     7621 ccagctcaat cttcgccaca tcacgcagca gtacgcggga accatcctga ttcactttca
     7681 gcaggatttt gccgaactct tcagtagagg tcagacgcgt ctgagcaata atagaggcgt
     7741 taagctgttg gcctttcacc ggcggcgtac caccgagctg acccgccgca acctgggcgt
     7801 tctgcgcttt gatggcggta atgacatcaa ccggcgttag ctggaatttg ttcagctcat
     7861 tcgggttcat ccagatacgc atcgcgtact gtgaaccgaa caactgaaca tcacccacgc
     7921 ccgacgtacg gctgatggca tctttcatat tcgccgctac gtagtcagag atatcctcct
     7981 gcgtcatggt gccatcggtg ttgataacgc cgacaaccat caggaagctg ctggatgatt
     8041 tctcaacgct caccccttgc tgctgaactt cttgcggcag caacggcatc gccagctgca
     8101 gtttgttctg cacctgaacc tgcgcgatat ccgcatcagt accagactca aaggtcaggg
     8161 tgatctgcac ggtacccgtg gagtcactgt tagaggacat gtacatcagg ttatcgatac
     8221 cgttcatatt ctgttcgata acctgtgtca ccgtgtcctg cactgttttc gcatcagcgc
     8281 cggggtagga ggcggagatc gttactgccg gcggtgcaat cgtaggatat tgcgccaccg
     8341 gcagtttgag gatcgccagc ccccctgcca acatgatgat aatggcgatc acccacgcaa
     8401 aaatcgggcg atcgataaag aaattaggca tgtcttaacg gctcctgttt aagttaagac
     8461 ttggactgtt caggctgagc accgcttgcg gcttgctggt tattatcagc ggtaacttct
     8521 tgtgctttta cctggacacc aggacgcact ttctgcagcc cacttattac tacgcgatcg
     8581 cctgctttca gaccttctgt caccagccac ttatcgccaa tagcctggct tgcaacgatc
     8641 ggacgggttt ccactttgtc atccgcgcca actaccagta cggtggcatc accacgcggc
     8701 gtacgggtta cgccctgttg cgggactaaa atagcgtttg gattaagccc ttcttccaga
     8761 cgtgcgcgca cgaacatacc cggcagcaga gtgtgatccg ggttcgggaa gatagcgcgt
     8821 agggtgatag acccagtggt ctgatcaacg gtaacgtcag agaattccag cgtaccgtcc
     8881 tgcgggaact taatgccgtc actggtgatc agcgacactt tggctttgcc gttctcttgt
     8941 ttcagcgtgc cattcgccag ttcctgtttc aggcgaagga agtcgttgct ggactgggtc
     9001 acatcaacgt agatcggatc aagttgctgc acggttgcca gcacagtcgc ctgaccgttc
     9061 tgtaccaatg cgccttccgt cacgttcgac ttaccaatgc gaccgctaat cggagaggtg
     9121 actttggtgt aagccagatt gatccgcgca gtttcaacgg cagctttcgc cgcagttacc
     9181 gcagcattcg cctgttgcgc atcagccaga gcctgatcgt acccttgctt actgatgtac
     9241 tgagtaccga gcagtttctg ataacgattc accgtcaatt gcgcgatatt ggctgcagcc
     9301 tgggctttcg ccagatcacc tttcgcactg tcgtatgtcg cctgataggt cgcaggatca
     9361 atctgataga gagagacacc agcttcgatg tcgctacctt ctttgaaatt acgcttcagg
     9421 ataatcccgc taacttgagg acgaacttct gcgatccggt aggcactggt gcgacctgga
     9481 agctcggttg tgatctgcag aggttcagtt ttgactgtta ctacgccaac ggcgggcatc
     9541 tgctggccac cttgttgggc ctgtttgtcg tcacatcctg ttagggctaa gctgcctgag
     9601 agcatcagaa cgaccgccag aggcgtaaac cctctgtttt tgttcatatg taaacctcga
     9661 gtgtccgatt tcaaattggt caatggtcaa aagttaataa acccattgct gcgtttatat
     9721 tatcgtcgtg ctatggtaca tacattcaca aatgtatgta aatctaacgc ctgtaaattc
     9781 acgaacatat ggcacgaaaa accaaacaag aagcgcaaga aacgcgccaa cacatcctcg
     9841 atgtggctct acgtcttttc tcacagcagg gggtatcatc cacctcgctg ggcgagattg
     9901 caaaagcagc tggcgttacg cgcggtgcaa tctactggca ttttaaagac aagtcggatt
     9961 tgttcagtga gatctgggaa ctgtcagaat ccaatattgg tgaactagag cttgagtatc
    10021 aggcaaaatt ccctggcgat ccactctcag tattaagaga gatattaatt catgttcttg
    10081 aatccacggt gacagaagaa cgacgtcgat tattgatgga gattatattc cacaaatgcg
    10141 aatttgtcgg agaaatggct gttgtgcaac aggcacaacg taatctctgt ctggaaagtt
    10201 atgaccgtat agaacaaacg ttaaaacatt gtattgaagc gaaaatgttg cctgcggatt
    10261 taatgacgcg tcgggcagca attattatgc gcggctatat ttccggcctg atggaaaact
    10321 ggctctttgc cccgcaatct tttgatctta aaaaagaagc ccgcgattac gtcgccatct
    10381 tactggagat gtatctcctg tgccccacgc ttcgtaatcc tgccactaac gaataaccct
    10441 gaatctgact ccaggatttt tcctggacat tttcgtcgtt gctattctgg ttcactgcgt
    10501 cgtgatattc ttgcggtttg actttttcag gtcgttcttc aggttcagaa accttcattc
    10561 atcatgacta tgttccagta ttacaaacga tcacggcatt ttgttttttc agcatttatt
    10621 gcttttgttt ttgtcttgtt atgccagaac acggcgtttg cgcgggcgtc atcgaatggt
    10681 gatctgccga caaaagcgga cctgcaggcg caacttgact cactaaataa acaaaaagat
    10741 ctttctgctc aggacaaact ggtgcagcag gatctgacag atacattagc caccctcgat
    10801 aaaatcgatc gcataaaaga agagacagtt cagctacggc aaaaagtcgc tgaagcgccg
    10861 gaaaaaatgc gccaggcgac cgcggcgtta acagcactta gcgatgtcga taacgacgaa
    10921 gaaacgcgca aaattctgag tacgctttcg ttgcgccagc tggaaactcg cgttgcccag
    10981 gcgctggacg atttgcaaaa cgcacaaaac gatctggcgt cttataacag ccagctggtt
    11041 tcgttacaga cgcagcccga acgcgtgcaa aatgcgatgt ataacgcttc gcagcagctg
    11101 caacaaattc gcagtcgtct ggatgggact gatgtcggcg agactgcctt acgtcccagc
    11161 cagaaagtgt taatgcaggc tcagcaggcg ttgctgaatg cggagattga ccagcagcgt
    11221 aaaagcctgg aagggaacac cgtcttgcag gataccttgc aaaagcaacg tgattacgtg
    11281 acggcgaaca gcgcccgtct ggagcaccag ttgcagctgt tgcaagaagc ggtaaacagc
    11341 aagcgcctga ctttaactga aaaaacggcg caggaagccg tctccccgga tgaagccgcg
    11401 cgtattcagg ctaatccgct ggtgaaacag gaactggaaa ttaaccagca gttaagtcag
    11461 cgtctgatta ccgcgactga aaacggtaat cagttgatgc agcaaaacat taaagtcaaa
    11521 aactggctgg agcgggcgct gcaatcggaa cgaaatatta aagagcagat tgccgtcctg
    11581 aagggcagcc tgctgttgtc tcgtatcctt taccagcaac aacaaacgct gccctcggcg
    11641 gatgaactgg aaaacatgac caaccgcatc gcggatttgc gtctcgaaca gtttgaagtt
    11701 aaccagcagc gtgatgcact cttccagagc gatgcgttcg tcaacaaact ggaagaaggt
    11761 cacaccaacg aagtcaacag cgaagttcac gatgcgttat tgcaagtggt tgatatgcgt
    11821 cgcgaattgc tggatcaact caacaaacag ttgggtaacc agctgatgat ggccattaac
    11881 ctgcaaatca accagcagca gttaatgagc gtatcgaaaa acctgaaatc catcctgact
    11941 cagcaaatct tttgggtgaa cagtaaccgt ccaatggact gggactggat caaagcgttc
    12001 ccgcaaagcc tgaaagatga atttaagtcg atgaaaatca cggtgaactg ggaaaaagcc
    12061 tggcctgccg tttttatcgc tttcctcgcc ggtttgccgc tgctgttgat tgccgggctg
    12121 atccactggc gtctgggctg gctgaaagcg tatcaacaga aactggcttc cgctgtgggt
    12181 tccctgcgta acgatagcca gctcaacaca ccaaaagcga tccttatcga cctgatccgc
    12241 gcgctgccgg tgtgcctgat tattctcgcg gttggcctga ttctgttgac catgcagctc
    12301 aacatcagcg aactgctatg gtcgttcagt aaaaaactgg cgattttctg gctggtgttt
    12361 ggcctgtgct ggaaggtact ggagaaaaat ggcgttgccg tgcgtcactt cggcatgccg
    12421 gagcagcaga ccagccactg gcgtcggcaa attgtccgca tcagtctcgc gttgctgcct
    12481 atccatttct ggtctgtggt ggcagaactt tccccgctgc atctgatgga tgatgtgctg
    12541 gggcaggcga tgattttctt caacctgctg ctgattgcct tcctggtatg gccaatgtgc
    12601 cgcgaaagct ggcgtgataa agagtcgcac accatgcgac tagtcaccat taccgtgctg
    12661 tcgattatcc cgattgcatt gatggtgctg actgccacgg gttatttcta caccacgctg
    12721 cgcctctccg ggcgttggat tgaaaccgtt tatctggtga tcatctggaa cttgctctac
    12781 cagacggtat tgcgcggctt aagcgtagcg gcgcggcgta tcgcctggcg tcgtgcgctg
    12841 gcgcgtcggc agaatctggt gaaagagggc gcagaaggtg ccgaaccgcc ggaagaaccc
    12901 accattgcac tggagcaagt taaccagcag acgctgcgta ttaccatgtt gctgatgttt
    12961 gcgctgttcg gtgtcatgtt ctgggcaatt tggtccgatt tgatcaccgt gttcagctat
    13021 ctcgacagca tcacgctctg gcattacaac ggcactgaag ctggcgctgc ggtggtgaaa
    13081 aacgtcacca tgggcagtct gttgtttgcg attatcgcct caatggtggc ctgggcgttg
    13141 attcgcaacc tgcctggttt actggaagtg ctggtgctct cgcgactgaa tatgcgccag
    13201 ggcgcgtcgt atgccattac taccatcctt aactacatca ttattgccgt tggtgcgatg
    13261 acggtgttcg gatcgctggg cgtctcttgg gataaactcc agtggctggc cgcagcatta
    13321 tccgtaggtc ttggttttgg tttacaagaa attttcggta acttcgtctc cggtttgatc
    13381 attctgttcg aacgtccggt gcgtattggc gatacggtaa ccattggtag cttctcgggg
    13441 acggtaagta agatccgtat tcgtgcgaca acgattaccg atttcgatcg caaagaagtg
    13501 atcatcccga acaaagcgtt tgttaccgag cgtctgatca actggtcgtt gactgacact
    13561 actacgcgtc tggtgatccg tctcggcgtg gcctatggct ccgatctgga aaaagtgcgt
    13621 aaagtgttac tgaaggcggc gactgagcac ccaagggtga tgcacgaacc aatgccggaa
    13681 gtcttcttta cggcatttgg tgccagcacg ttggatcatg agctgcgtct gtatgtgcgt
    13741 gaactgcgtg accgtagccg tactgtcgat gagctgaacc gtactatcga tcagctgtgc
    13801 cgtgaaaacg acatcaacat tgcctttaac cagcttgaag tgcatctgca caacgagaag
    13861 ggcgatgagg tgacggaagt aaaacgcgac tacaaaggcg acgatccgac accagcggtt
    13921 gggtaataac caaagggggc gacatttagt tgccccgaga ttactgacaa agtgcgcgtt
    13981 gttcatgccg gatgcggcgt gaccgcctta tccggcctac gaaaccgcaa gaattcaata
    14041 tattgcagga gcggtgtagg cctgataagc gtagcgcatc aggcagtttt gcgtttgccc
    14101 gcaatcttag gggacattta gcggccccat ttatttctca cttttccgcc tcatcatcgt
    14161 gcgttaattt cttttcataa tcacgcttta caatatccag cgcgcgcagc acggtactgg
    14221 cagggatctg attttcctcc agcagcacaa tcaaatcgac agccagtttg acatcgtcag
    14281 gggcattttc aagtgacata ctctttccat tgctagcggg ttaagcgcgc tagcctgttt
    14341 tcgatttttt ccagcgcatg gcggcagcgt gccaggcgcg cttcataggc ttccacttca
    14401 cgatgcagcg tttgctgttc cacgagatcg gtcacccgcg ccaggcgggc tctgcgttcg
    14461 gcaaccatct cacgtagccg ccgctcaaaa tcctgatgct gaatacgttt acgctgccag
    14521 cgggaaattt tcggtggcgc actgtcccac tcgcgcaatg accaggcgga ggcttcacgc
    14581 gcgatggctt ccagttgtgc cgccagatgt tccgccagcc aggccacttg cggcagctgt
    14641 tgctgctcaa ctgcatgacg aagcgcagcc agattatcgc ccgcctcgtc gagacaagac
    14701 tgtagtgttg tcgcacgagt ctgaaaaaga tgcctgtcga aacgagcact tagcgtggcg
    14761 aactgtgaca ccggggcaca acgctgacgc agcgtagcga gctgtccttc cagtttttcc
    14821 agcagcaggg cggttttcac gataagacct caatgaaaat gataattgtt atgctaaagt
    14881 agccactatg taagctgaca acactcaata tgcaacgaat cattttaatc atcattggct
    14941 ggctggcggt agtgctgggt acgctgggcg tggtattacc ggtattaccg acgacgccgt
    15001 ttatcctgct ggcggcctgg tgctttgccc gttcttcccc gcgctttcac gcctggttgc
    15061 tgtaccgctc atggtttggc agctatctac gtttctggca gaaacatcat gcgatgccgc
    15121 gcggcgtcaa accgcgggcg attttgctta ttttgctcac gtttgccatt tctctgtggt
    15181 tcgtccagat gccatgggtg cgcatcatgt tgctggtaat tctcgcctgt ttgcttttct
    15241 atatgtggcg aattccggtg attgatgaaa agcaagaaaa gcactgaagc acaacaatcg
    15301 cagttgcaat tattgcgtac agccagtaca ttctggcgtt ttcgagcaca ggcgcaggcg
    15361 gtcaaaggtt aaacaacagt tacttttgat acgtttaaaa cgcgccgtga gtaccaccgt
    15421 aacaagcagg catacactta tgaccgcgac tgcacagcag cttgagtatc tcaaaaatag
    15481 catcaaaagc attcaggact acccaaaacc cggcattctt ttccgcgatg tcaccagctt
    15541 actggaagac ccgaaagctt acgctctcag catcgacttg ctggttgagc gttacaaaaa
    15601 tgcgggcatt accaaagttg tcggcaccga agcgcgtggc ttcttgtttg gcgctccggt
    15661 agctctgggt ctgggcgttg gctttgtacc ggtccgtaaa ccgggcaaac tgccgcgtga
    15721 aaccatcagt gaaaattacg acctggaata cggcaccgat cagctggaaa tccacgttga
    15781 tgccatcaaa ccgggcgaca aagttctggt ggtggacgat ctgctggcaa ccggcggcac
    15841 tatcgaagcg accgttaaac tgatccgtcg tctgggtggt gaagtggctg acgctgcgtt
    15901 cattatcaac ctgtttgatc tcggcggcga acagcgtctc gaaaaacagg gcattaccag
    15961 ctacagcctt gtcccgttcc cgggccatta attatcgcca gtcttgtgct gcccacgcta
    16021 cggacagcac aagatgtgca ttcagcctcg ccgttctgac ggggctgtgt tagcattacc
    16081 ccttcgtgaa tccaccttcc agcgtttcag agcctgccaa tgagttatca ggttttagcc
    16141 cgaaaatggc gcccacaaac ctttgctgac gtcgtcggcc aggaacatgt gctgaccgca
    16201 ctggcgaacg gcttgtcgtt agggcgtatt catcatgctt atcttttttc cggcacccgt
    16261 ggcgtcggaa aaacctctat cgcccgactg ctggcgaagg ggctaaactg cgaaaccggc
    16321 attaccgcga cgccgtgcgg cgtgtgcgat aactgtcgtg aaatcgagca ggggcgcttt
    16381 gtcgatctga ttgaaatcga cgccgcctcg cgcaccaaag ttgaagatac ccgcgacctg
    16441 ctggataacg tccagtacgc tccggcgcgt ggtcgtttca aagtttatct gatcgacgaa
    16501 gtgcatatgc tgtcgcgcca cagctttaac gcactgttaa aaacccttga agagccgccg
    16561 gagcacgtta agtttctgct ggcgacgacc gatccacaga aattgccggt gacgattttg
    16621 tcacgctgtc tgcaatttca tctcaaggcg ctggatgtcg agcaaattcg ccatcagctt
    16681 gagcacatcc tcaatgaaga acatatcgct cacgagccgc gggcgctgca attgttggcg
    16741 cgcgccgctg aaggcagcct gcgagatgcc ttaagtctga ccgaccaggc gattgccagc
    16801 ggtgacggcc aggtttcaac ccaggcggtc agtgcgatgc tgggtacgct tgacgacgat
    16861 caggcgctgt cgctggttga agcgatggtc gagggcaacg gcgagcgcgt aatggcactg
    16921 attaatgaag ccgctgcccg tggtatcgag tgggaagcgt tgctggtgga aatgctcggc
    16981 ttgttgcatc gtattgcgat ggtacaactt tcgcctgctg cacttggcaa cgacatggcc
    17041 gccatcgagc tgcggatgcg tgaactggcg cgcaccatac cgccgacgga tattcagctt
    17101 tactatcaga cgctgttgat tggtcgcaaa gaattaccgt atgcgccgga ccgccgcatg
    17161 ggcgttgaga tgacgctgct gcgcgcgctg gcgttccatc cgcgtatgcc gctgcctgag
    17221 ccagaagtgc cacgccagtc ctttgcaccc gtcgcgccaa cggcagtaat gacgccaacc
    17281 ctggtgccgc cgcaatcagc gccgcagcag gcaccgactg taccgctccc ggaaaccacc
    17341 agccaggtgc tggcggcgcg ccagcagttg cagcgcgtgc agggagcaac caaagcaaaa
    17401 aagagtgaac cggcagccgc tacccgcgcg cggccggtga ataacgctgc gctggaaaga
    17461 ctggcttcgg tcaccgatcg cgttcaggcg cgtccggtgc catcggcgct ggaaaaagcg
    17521 ccagccaaaa aagaagcgta tcgctggaag gcgaccactc cggtgatgca gcaaaaagaa
    17581 gtggtcgcca cgccgaaggc gctgaaaaaa gcgctggaac atgaaaaaac gccggaactg
    17641 gcggtgaagc tagcggcaga agccattgag cgcgacccgt gggcggcaca ggtgagccaa
    17701 ctttcgctac caaaactggt cgaacaggtg gcgttaaatg cctggaaaga ggagagcgac
    17761 aacgcagtat gtctgcattt gcgctcctct cagcggcatt tgaacaaccg cggtgcacag
    17821 caaaaactgg ctgaagcgtt gagcatgtta aaaggttcaa cggttgaact gactatcgtt
    17881 gaagatgata atcccgcggt gcgtacgccg ctggagtggc gtcaggcgat atacgaagaa
    17941 aaacttgcgc aggcgcgcga gtccattatt gcggataata atattcagac cctgcgtcgg
    18001 tttttcgatg cggagctgga tgaagaaagt atccgcccca tttgatcgta agcacagctt
    18061 acgttcgtca tccttaacgt gattgagaga gaaacctatg tttggtaaag gcggtctggg
    18121 taacctgatg aagcaagccc agcagatgca agaaaaaatg cagaaaatgc aggaagagat
    18181 cgcgcagctg gaagttaccg gcgaatctgg cgcaggtctg gtaaaagtga ccatcaacgg
    18241 tgcacacaac tgccgtcgcg tagagatcga cccgagcctg ctggaagacg acaaagagat
    18301 gctggaagac ctggtggctg cagcattcaa cgacgcagca cgtcgtattg aagaaacgca
    18361 gaaagaaaaa atggcctctg tatcctccgg aatgcagctg ccgcctggct ttaagatgcc
    18421 gttctgatgc aaaccagccc gctgttaaca cagcttatgg aagcactgcg ctgtctgccg
    18481 ggcgttggcc cgaagtcggc gcagcgtatg gcgttcacgc tgcttcagcg cgatcgtagc
    18541 ggcgggatgc gtctggcgca ggcgctcacc cgggcgatgt cggaaatcgg ccactgcgcc
    18601 gattgccgca ctttcaccga acaggaagtc tgtaacatct gttcgaatcc gcgtcgtcag
    18661 gaaaacggtc aaatctgtgt ggtggagagt ccggcggaca tctacgccat tgagcagacg
    18721 gggcagtttt ctggtcgtta ttttgtgttg atggggcatc tgtcaccgct ggacggcatc
    18781 ggtccggatg atatcgggct tgatcgtctg gaacagcgtc tggcagagga aaaaatcact
    18841 gaagtgatcc tcgccaccaa tcccacggtt gaaggtgaag ctaccgctaa ctacattgcc
    18901 gagctttgcg cgcaatatga cgtggaagcc agccgaatcg ctcatggcgt accggttggc
    18961 ggcgaactgg aaatggtcga tggcaccacg ctgtcacact ccctcgccgg gcgtcataag
    19021 attcgttttt aagcaaacga gagcaggagc acctgctctc gcttgaaatt attctccctt
    19081 gtccccatct ctccctcatc ctgtttttaa ccttaaaatg gcattattga ggtagaccta
    19141 catgaaagga caagaaactc gtggttttca gtcagaagtg aaacagcttc tgcacctgat
    19201 gatccattct ctctattcca ataaagaaat cttcctgcgt gagcttatct ctaacgcctc
    19261 cgatgcggcg gacaagctgc gtttccgtgc gctatctaac ccggacctgt acgaaggtga
    19321 tggcgaactg cgcgttcgtg tctctttcga taaagacaag cgcacgctga ccatctccga
    19381 taacggcgtg gggatgaccc gcgacgaagt gattgaccat ctggggacta tcgctaaatc
    19441 cggtaccaaa tcattcctcg aatccctggg ttctgaccaa gcgaaagaca gccagctgat
    19501 cggtcagttt ggtgttggtt tctactctgc gtttatcgtg gccgacaaag tgaccgtgcg
    19561 tactcgcgcg gcaggcgaaa aaccagaaaa cggcgtcttc tgggaatcgg ctggcgaagg
    19621 tgaatatacc gttgccgata tcaccaaaga agatcgtggt actgaaatca ccctgcatct
    19681 gcgtgaaggc gaagacgagt tcctcgatga ctggcgcgtg cgttccatca tcagcaaata
    19741 ctccgaccat atcgcgctgc cggtagagat cgaaaaacgc gaagagaaag atggcgaaac
    19801 cgttatctcc tgggcaaaaa tcaacaaagc gcaggcgctg tggactcgta acaagtcgga
    19861 aatcaccgat gaagagtata aagagttcta caaacacatc gcccacgact ttaacgatcc
    19921 gctgacctgg agccacaacc gcgttgaagg taagcaggag tacaccagcc tgctgtacat
    19981 cccgtcccag gctccgtggg atatgtggaa ccgtgatcat aaacacggcc tgaaactgta
    20041 cgttcaacgc gtgtttatca tggacgacgc tgaacagttc atgccgaact atctgcgctt
    20101 cgtgcgtggt ctgattgact ccagcgatct gccgctgaac gtttcccgtg aaatcctcca
    20161 ggacagcacg gtaacgcgta acctgcgcaa tgcgctgacc aagcgtgtgc tgcaaatgct
    20221 ggaaaaactg gcgaaagacg acgcggaaaa ataccagacc ttctggcaac agtttggcct
    20281 ggtactgaaa gaaggtccgg cggaagattt cgctaaccag gaagcgatcg ccaaactgct
    20341 gcgttttgct tctacttata ccgattcttc tgcgcagacc gtgtctctgg aagactacgt
    20401 ttcccgcatg aaagaagggc aggagaaaat ctactacatc actgcagaca gctatgcggc
    20461 agcgaagagc agcccgcacc tggaactgct gcgtaagaaa ggcatcgaag ttctgctgct
    20521 ttccgaccgc atcgatgagt ggatgatgaa ctatctgact gagttcgacg gtaaaccgtt
    20581 ccagtcggtg tctaaagttg acgagtcgct ggaaaaactg gctgacgaag ttgatgagag
    20641 cgcgaaagaa gcggagaaag cactgactcc gttcatcgac cgcgtgaaag ctctgctcgg
    20701 cgagcgcgtg aaagatgtcc gtctgactca ccgtctgacc gatacgccag cgatcgtttc
    20761 gaccgacgcg gacgaaatga gcactcagat ggcgaaactg ttcgctgcgg cgggccagaa
    20821 agtgccagaa gtgaaataca tcttcgaact gaacccggat cacgtactgg tgaaacgtgc
    20881 ggcagatact gaagatgaag ccaagttcag cgagtgggta gaactgctgc tggatcaggc
    20941 gctgctggca gaacgcggca cgctggaaga tccgaacctg tttattcgtc gtatgaatca
    21001 gctgctggtt tcctgatgtc atgccggatg acctttgtgt catccggcat ttttcttttc
    21061 atcatctgta ctttccgcaa attatctcgc cattaaccgt ttcagcctca ggtgcctttc
    21121 ttgaggcaat cgcctgttgg tggtatcgtt tatcgctttt tcaaaaaaat cgacacattt
    21181 taaggggatt ttcgcaatgc gtatcattct gcttggcgct ccgggcgcgg ggaaagggac
    21241 tcaggctcag ttcatcatgg agaaatatgg tattccgcaa atctccactg gcgatatgct
    21301 gcgtgctgcg gtcaaatctg gctccgagct gggtaaacaa gcaaaagaca ttatggatgc
    21361 tggcaaactg gtcaccgacg aactggtgat cgcgctggtt aaagagcgca ttgctcagga
    21421 agactgccgt aatggtttcc tgttggacgg cttcccgcgt accattccgc aggcagacgc
    21481 gatgaaagaa gcgggcatca atgttgatta cgttctggaa ttcgacgtac cggacgaact
    21541 gattgttgat cgtatcgtag gccgccgcgt tcatgcgccg tctggtcgtg tttatcacgt
    21601 taaattcaat ccgccgaaag tagaaggcaa agacgacgtt accggtgaag aactgactac
    21661 ccgtaaagac gatcaggaag aaaccgtgcg taaacgtctg gttgaatacc atcagatgac
    21721 tgcaccgctg atcggctact actccaaaga agcggaagcg ggtaacacca aatacgcgaa
    21781 agttgacggt accaagcagg ttgctgaagt tcgcgctgat ctggaaaaaa tcctcggcta
    21841 atcaagccgc agcagatctg ctttcgggca ggtctgctta tccacctcag gcaatacctc
    21901 tcacagcaat tagttcttct tcctcacttt tccgctacaa ttatcaacaa gttgaatcga
    21961 taagaggcgg taatgcgtca gactaaaacc ggtatcctgc tggcaaacct gggtacgccc
    22021 gatgccccca cacctgaagc ggtaaaacgc tatctgaaac aatttttaag cgacagacgc
    22081 gtggttgata cctcacggtt gttatggtgg ccgttgctgc gcggcgtgat tttgccgctg
    22141 cgctcgccgc gtgtggcgaa gctgtacgcc tctgtctgga tggaagacgg ctcgccgctg
    22201 atggtttaca gccgccagca acagcaggcg ttggcacaac gtttaccgga tacgccagtg
    22261 gcgctgggca tgagctacgg ctcgccatca ctggaaagct ccgtcgatga actcctggca
    22321 gagcatgtag atcatattgt ggtgctgccg ctttatccgc aattctcctg ttctacggtc
    22381 ggtgcggtat gggatgaact ggcacgcatt ctggcgcgca aacgtagcat tccggggata
    22441 tcgtttattc gtgattacgc cgataaccac gattacatta atgcactggc gaacagcgta
    22501 cgcgcttctt ttgccaaaca tggcgaaccg gatctgctgc tgctctctta tcatggcatt
    22561 ccccagcgtt atgcagatga aggcgatgat tacccgcaac gttgccgcac aacgactcgc
    22621 gaactggctt ccgcactggg gatggtaccg gaaaaagtga tgatgacctt tcagtcgcgc
    22681 tttggccggg aaccctggct gatgccttat accgacgaaa cgctgaaaat gctcggagaa
    22741 aaaggcgtag gtcatataca ggtgatgtgc ccgggctttg ctgcggattg tctggagacg
    22801 ctggaagaga ttgccgagca aaaccgtgag gtcttcctcg gtgcgggcgg gaaaaaatat
    22861 gaatatattc cagcgcttaa tgccacgccg gaacatatcg aaatgatggc taatcttgtt
    22921 gccgcgtatc gctaaagctg agcggtaaag aactgagcgc cgtcgcgaag agcctcgtcg
    22981 gcggttttca tcatccgtga ataatgcaaa aaggcatgca gcgtacctgg gtagagtttg
    23041 aactcacagg gctgctgatg cgccgctaac gtctagtaaa gcagacggct gtcatccagc
    23101 agcggatcga actccgcccc ggcaataaaa cagggcggaa cttcgcgagt gagatcatta
    23161 ttaaacagac agtaatacgg tgactcgcgg tccgcgtcgt tgcttaaata cgcctcttca
    23221 tacatctgca aatcctgttg cgttaagcca tcccagacac cgcccaacag acgacgagtc
    23281 acggaatccc gtaatccgta aagcccatac cacagcaaaa cacccgcaat tttaccgcaa
    23341 tcgatctgtt tatcacgcaa ccacaacgca ctggcgagcg ccagcatggc acctgcggaa
    23401 tcaccggcaa agccaatgcg ggacatattg atttgataat cctccgcctg ctggtggaaa
    23461 taacaacaag cagccacaat ttcctctatc gcttgtggaa aacgcgcttc aggtgaaagg
    23521 gtgtaattaa taccaatcac cgtacattgg ctgtagcttg ccagcaggcg catgatgcga
    23581 tcgtgggtat cgagattgcc gagaataaaa ccgcctccat gcaaataaaa tagcgtcgcc
    23641 gggctatctg gctgcggaca aaagagacgt gtttccacct gcccatattt tgttggaacc
    23701 atgtaagctc tggttgccat ttctggagcg cccgcattcc agaatcggcg ctcaagcgtg
    23761 taatactgtc gttgctcagc aatcgttccc gttgcgggcc aggacggtaa atccggctga
    23821 agagtattca caacggtctt catttcagca gaaataaggt ccagaacagg tagtttgttt
    23881 tccggcttca tacaaaaact cctttcaaat tacgtcattg taaggaacca ctgccatgaa
    23941 aatgcgatcc cgcctgctga tattgaaact ggctgcgtct cgcgcgctcc cgtcagattg
    24001 tgttaacatt cgccgctcag ttaaccaccc gtaaaaacaa ccatgaaatt tcccggtaaa
    24061 cgtaaatcca aacattactt ccctgtaaat gcacgcgatc cgctgcttca gcaattccag
    24121 ccagaaaacg aaaccagcgc cgcctgggtc gtaggtattg accagacgct ggtcgatatt
    24181 gaagcgaaag tggatgatga attcattgag cgttatggat taagcgccgg gcattcactg
    24241 gtgattgagg atgacgtagc cgaagtgctt tatcaggagc taaaacagaa aaacctgatt
    24301 acccatcagt ttgcgggtgg caccattggt aacaccatgc acaactactc ggtgctcgcg
    24361 gacgaccgtt cggtgctgct gggcgtcatg tgcagcaata ttgaaattgg cagttatgcc
    24421 tatcgttacc tgtgtaacac ttccagccgt accgatctta actatctaca aggcgtggat
    24481 ggtccgattg gtcgttgctt tacgctgatt ggcgagtccg gggaacgtac ctttgctatc
    24541 agtccaggcc acatgaacca gctgcgggct gaaagcattc cggaagatgt gattgccgga
    24601 gcctcggcac tggttctcac ctcatatctg gtgcgttgca agccgggtga acccatgccg
    24661 gaagcaacca tgaaagccat tgagtacgcg aagaaatata acgtaccggt ggtgctgacg
    24721 ctgggcacca agtttgtcat tgccgagaat ccgcagtggt ggcagcaatt cctcaaagac
    24781 cacgtttcta tccttgcgat gaacgaagat gaagccgaag cgttgaccgg agaaagcgat
    24841 ccgttgttgg catctgacaa ggcgctggac tgggtagagc tggtgctgtg caccgccggg
    24901 ccaatcggct tgtatatggc gggctttacc gaagacgaag cgaaacgtaa aacccagcat
    24961 ccgctgctgc cgggcgctat agcggaattc aaccagtatg agtttagccg cgccatgcgc
    25021 cacaaggatt gccagaatcc gctgcgtgtc tattcgcaca ttgcgccgta catgggcggg
    25081 ccggaaaaaa tcatgaacac taatggagcg ggggatggcg cattggcagc gttgctgcat
    25141 gacattaccg ccaacagcta ccatcgtagc aacgtaccaa actccagcaa acataaattc
    25201 acctggttaa cttattcatc gttagcgcag gtgtgtaaat atgctaaccg tgtgagctat
    25261 caggtactga accagcattc acctcgttta acgcgcggct tgccggagcg tgaagacagc
    25321 caggaagagt cttactggga tcgttaagtt atcgtcggtt cgtaggccag ataaggcgtt
    25381 cacgccgcat ctggcatttg gctctcgatg cctgatgcga cgctggcgcg tcttatcatg
    25441 cctacatatt tttcatattt tacatccggc aaccaccgtt taccccgtca ccacttcacc
    25501 cgccggtggc gtttccagca gttccagcat agtacgggca atttcacgtt cacccatcac
    25561 tacctgattc gcgccacgtt cggtgatata cgtcacttca tcgtcataat gggcgcgggc
    25621 gataatctca atatccgggt ttttcgcgcg ggcagacgcc acaatctcac ccgcttcata
    25681 accgttgggg atcgtcagga tcagccattt tgcacattcc agatgtgcca gttgcataat
    25741 ttcttcgttc gctgcattgc ccaatacagc gcggacccca cgctctcgca gctcatcaac
    25801 acgggttcgt gacgtctcaa tcaccaccag cggaatatca gaggcgagca atttctcccc
    25861 cagcaggctg cctacacgac cgtaacccac caatagcgca tggttgcaaa tatccactgg
    25921 gatctgtttc tcttcttcga ttgcctcttc cagcgtctgc tcttccagcg tttcggtctt
    25981 cgccagatat ttctccagta gtgcgaacag taccgggttg agcataatcg acaggatcgc
    26041 ccctgccagt accaggtttt gtccggcctg cggcagtaaa ttcaatgcca ttcccagtcc
    26101 cgccaggata aacgcgaact caccaatctg cgccaggctg gcggcgatgg ttaatgccgt
    26161 acgttgggag tgaccaaaca gacggacaag gaacagggcc gcgaccgatt taccaaacag
    26221 aataattgcc agcgtcgcca gcactgccag cggttgctga atcagaatta acggatcaaa
    26281 caacatcccg actgagacaa aaaacagcac cgcaaacgcg tcgcgcaatg gcaacgtatc
    26341 gtgggcggca cggtgactca attcagactc gttcagtacc atcccggcaa agaacgcacc
    26401 gagtgcaaag gagacatcaa acagctctac cgcaccaaag gcaatcccta acgccagcgc
    26461 cagcactgac agggtaaaca gctcgcgaga accggttgcc gcgctgcgtg ccataatcca
    26521 cggcaccaga cggcgaccta ccagcatcat aatggcgata aatgcgatca ccttgccgat
    26581 ggtgatcccc atatcgactg caagagtggc aaagcccaca tcgccctgtt ccatcattcc
    26641 tgccactgcg ggcagcaaca ccagcgtcag aaccatcacc aggtcttcca caatcaacca
    26701 accgatggcg atttgcccac gctgactgtc tattaattgc cgttcttcaa gtgcgcgcag
    26761 taacaccacg gtactggcgg tggaaagaca taagccgaac acgataccgg tcattaacga
    26821 ccagcccagc acggcggaga gcgccatacc cagcagcgtc gccacggcta tctgggcgat
    26881 cgcgccggga atggcgatgg actttaccgc catcaaatcc ttgagcgaaa aatgcaaacc
    26941 gacgccgaac atcagcaaaa taacgcccag ttcagccagt tccggcgcaa gcttggtatc
    27001 ggcaacaaag cccggagtga atggtcctgc cagcacaccc gctaacagat atcccaccag
    27061 aggagaaata cgtagtttat tggccagcat gccgaggata aaggcgagca caaggccgcc
    27121 aacaatggtg gtgataagcg gggtggcgtg atgcattccg tctccttttc ctggtggtta
    27181 ttgtccattt ttggctggga aaaccaaaat tacaggtaat agtttatgac aatttcattg
    27241 atgatgttta tgaataattg ttgaattttg cagaaaaatg gaattagctg caaaaaaagc
    27301 acggttcgga aaacagaagg cggtttaaca gaggaaaggc ttatggcgac tgtaaaacga
    27361 tgcagccaaa gtttgccttt ggctgcaatg aaatcagtct ttatgccggt tatcaggcag
    27421 gaatatggtc aacatcccca atagtggcag gaaagcacag attttataga ctaactcgat
    27481 gctggtgtga tcggcgataa gccccagaac tgccgctccc agacctccca tgccgaaagc
    27541 aaaaccgaaa aagagtccag aaaccatacc gatacgtccc ggaagtagct cctgagcgta
    27601 gaccagaatg gcagagaatg ccgaagcgag gataaatcca ataatcaccg ttaaaacccc
    27661 cgtccagtgc aggcgggcgt agggtaaaat cagcgtaaac ggcgcaacgc cgaggataga
    27721 gccccaaatc acatatttcc gtccaatttt atcccctaca ggcccgccga tcaccgtacc
    27781 tgccgcaacg gcaaacagga aggcaaacag atgaagctgg gcattctgga tagataatcc
    27841 gaatttttgc atcagataaa aggtgtaata gctgctgatg ctcgccatat agaaatattt
    27901 cgagaaaatg aggattaaca gaatgctgac tgccagtacc actttgttgc gcggcagtgg
    27961 attgattatc gtcgctttgg gttttccttt attcattcgg tgctgtgccg agtaccaacg
    28021 gctaatttgc gccaacacca cgatcgccag cagtgccgca agcacaaacc aggcaacgtt
    28081 gcctttgcca taaggctcga taatcaccgc cgccagcaag ggtcccaggg aactgccaaa
    28141 gttgccgccg acctgaaaga tagattgcgc caggccatgc cgcccgccgg aagccatacg
    28201 ggctacgcga gaagattccg gatgaaagac cgatgaaccg gtaccgacca gcgccgctgc
    28261 cagcagaact gcgccaaaac tgcccgccag cgcaagcagc accagaccac ttaaggtaaa
    28321 gcacatgcca attggcaacg accacggcat cggatattta tcggtccagt agccgaccac
    28381 tggttgcagt agcgaagagg cgagctggaa ggtgagggtt atcatgccaa tctgcataaa
    28441 tgtcagagaa aattctgact gaagcagcgg ataaatcgcc agaatcagcg attggatcat
    28501 gtcgttcagc agatgtgaga ggctgatagc acctaaaata ccaaacgatg ttcgggcctt
    28561 ggtcgttgac gcagccgcgc ccgccacagg ctgggtttgt tcactcattg ccataggaaa
    28621 gtcacttttt cagggttgcg atgtaaagaa tgatcttatt tgtgattatt acccgactaa
    28681 catacctgta tgcgtcgtct gaaggaagtc tcaacgccga atacagaatt tctaatctgg
    28741 atgcagattt atcttcaccg gacgcagact tgtctatgat gttgcgtcat actatttttc
    28801 aacacgttga aatcaggtca gggagagaag tatgaaatta ttgcagcggg gcgtggcgtt
    28861 agcgctgtta accacattta cactggcgag tgaaactgct ctggcgtatg agcaggataa
    28921 aacctacaaa attacagttc tgcataccaa tgatcatcat gggcattttt ggcgcaatga
    28981 atatggcgaa tatggtctgg cggcgcaaaa aacgctggtg gatggtatcc gcaaagaggt
    29041 tgcggctgaa ggcggtagcg tgctgctact ttccggtggc gacattaaca ctggcgtgcc
    29101 cgagtctgac ttacaggatg ccgaacctga ttttcgcggt atgaatctgg tgggctatga
    29161 cgcgatggcg atcggtaatc atgaatttga taatccgctc accgtattac gccagcagga
    29221 aaagtgggcc aagttcccgt tgctttccgc gaatatctac cagaaaagta ctggcgagcg
    29281 cctgtttaaa ccgtgggcgc tgtttaagcg tcaggatctg aaaattgccg ttattgggct
    29341 gacaaccgat gacacagcaa aaattggtaa cctggaatac ttcactgata tcgaatttcg
    29401 taagcccgcc gatgaagcga agctggtgat tcaggagctg caacagacag aaaagccaga
    29461 cattattatc gcggcgaccc atatggggca ttacgataat ggtgagcacg gctctaacgc
    29521 accgggcgat gtggagatgg cacgcgcgct gcctgccgga tcgctggcga tgatcgtcgg
    29581 tggtcactcg caagatccgg tctgcatggc ggcagaaaac aaaaaacagg tcgattacgt
    29641 gccgggtacg ccatgcaaac cagatcaaca aaacggcatc tggattgtgc aggcgcatga
    29701 gtggggcaaa tacgtgggac gggctgattt tgagtttcgt aatggcgaaa tgaaaatggt
    29761 taactaccag ctgattccgg tgaacctgaa gaagaaagtg acctgggaag acgggaaaag
    29821 cgagcgcgtg ctttacactc ctgaaatcgc tgaaaaccag caaatgatct cgctgttatc
    29881 accgttccag aacaaaggca aagcgcagct ggaagtgaaa ataggcaaaa ccaatggtcg
    29941 tctggaaggc gatcgtgaca aagtgcgttt tgtacagacc aatatggggc ggttgattct
    30001 ggcagcccaa atggatcgca ctggtgccga ctttgcggtg atgagcggag gcggaattcg
    30061 tgattctatc gaagcaggcg atatcagcta taaaaacgtg ctgaaagtgc agccattcgg
    30121 caatgtggtg gtgtatgccg acatgaccgg taaagaggtg attgattacc tgaccgccgt
    30181 cgcgcagatg aagccagatt caggtgccta cccgcaattt gccaacgtta gctttgtggc
    30241 gaaagacggc aaactgaacg accttaaaat caaaggcgaa ccggtcgatc cggcgaaaac
    30301 ttaccgtatg gcgacattaa acttcaatgc caccggcggt gatggatatc cgcgccttga
    30361 taacaaaccg ggctatgtga ataccggctt tattgatgcc gaagtgctga aagcgtatat
    30421 ccagaaaagc tcgccgctgg atgtgagtgt ttatgaaccg aaaggtgagg tgagctggca
    30481 gtaatccgaa agtgccggat gtttgcatcc ggcacaatgc ttaatcgcgg cgggcgatat
    30541 cagcaaattt ggcatcgagg atctttgcca gatcgcctgc cgccagttcg atatccagtc
    30601 cgcgcttgcc gccggaaaca taaatagtgg caaattcttg tgcgggggcg tcgataatcg
    30661 ttggcagacg ttttttctgc cccagtgggc taatcccccc aaccaggtat cccgtcgaac
    30721 gctgcgcgac catcggatcg gccatctcaa ctttcttggc acccagcgct tttgctactt
    30781 ttttaagatc cagttgacct gcgaccggcg taacggccac ggcaaggtgt ttcatatcac
    30841 cgttcactgc caccagcagc gttttgtaga cctgatccgg attcaaacct aattttttga
    30901 cgacttcatc gccaaaattg gtttcagccg gatcgtgctc gtaggtatgg atttgaaacg
    30961 aaatcttgtt tttttcgagt aatttaactg cgggtgtcat ggtaattttc ctgccttaaa
    31021 caaaaaaacg aagcaagcat acgcctgatg gcggtccaaa aaaaagagtc atcttgccta
    31081 agagtattgg caggatggtg agattgagcg acaatcgagt tacaccgtcg cttaaagtga
    31141 cggcataata ataaaaaaat gaaattcctc tttgacgggc caatagcgat attggccatt
    31201 tttttagcgc aacatttgcg gcaaattccc ttctccatac aggtgtagtg caccgaccgc
    31261 gaccacatat cgccccggcg gcatggcgcg taatttatcc cgccaggcga gatttcgctg
    31321 atgcatcagc acatcgtaca gcgactgact gaacgtattg ggcagcgtta tatcattatt
    31381 ttgcggcggt gcattcagcc accagctcat catttgttgc agcaaccgtg cgttggtatg
    31441 ccagtgggtc agcgtatcgt ccagcagcgc cagtcctttg tcagggagct ggagcaacat
    31501 ggcaatctgg ttttcagccc cttccagttc aatcacgggt ttatgttgtt gcttcgccgc
    31561 ctgcaatagc tggtaatcga taccgtattc tgcccgcagc cccagttttt gtgcctgcgt
    31621 cgcctgaaga accatcgcga tttgccacag cggttgggta gaaaagagtg agggagaaat
    31681 gcccatctcc tggctaatgt gctgcaggtt ttggagttgc tcctcgctaa tgcgctcttc
    31741 cagcgcctcg caggcaggca aattagcaaa aggcgtatcg ctggtggaaa catccgcctc
    31801 gacgatcagc gcatcggcgt ttttgagctt tttgagcaaa cgggtgggca ggggagccat
    31861 atcgtggcta cccatatgaa tactgccaat cagatgaaaa tggcgattgc cggggagggt
    31921 gatatcgatg gcaggccagg tgtaatgatt accgcgcagc gcggcccaaa gcgtttttac
    31981 ccggtacaac agatccatac gacctccctt tgtgaaatat catgctagcg cgcggtgagg
    32041 gatggcgcaa atggagttgg ggacttgttt aatgtgtttg tatgattcag tatgttcttg
    32101 catcgctatt cacagggaag caacagttaa aaaccatgaa acaggcaaca agaaaaccga
    32161 cgacacccgg agatattctt ctctatgaat atctggaacc gctcgatttg aaaatcaatg
    32221 agttagcaga gttgctgcat gttcatcgta atcgcgtcag tgcactgatc aataacaatc
    32281 gtaaactcac cactgagatg gcattccgtc tggcgaaagt ttttgatacc acagtcgatt
    32341 ttaggctaaa cctccaggcg gcagttgatc tttgggaagt tgaaaacaac atgcgcaccc
    32401 aggaagaatt gggacggatt gaaacagtgg ctgaatattt ggcacgccgt gaagagcgtg
    32461 caaaaaaggt cgcgtaaggc caaaacatcg cttcatctcg taggccggat aaggcgttca
    32521 cgccgcatcc ggcatccgag caccattgcc tgatgcgacg cttacgcgtc ttatcaggcc
    32581 tacaaacctg tgcctgaacc gtaggccgga taaggcgttt acgccgcatc cggcagtcat
    32641 gcgtcgatgc caaatgcgcc gccctaaagc agcgcatccg caatgacgta cttattcctt
    32701 cggtttaaac cgcagcaacc ggttggcgtt acttactacg gtaattgacg agagcgccat
    32761 tgccgctccg gcaactaccg ggttaagcag tgttccagta aacggccaca aaataccggc
    32821 ggcgaccgga ataccgatgc tgttgtagat aaaggcaccg agcaggttct gcttcatgtt
    32881 gcgcagcgtt gcgcgggaaa tcgcgagcgc atccgcaacg cccatcaggc tatggcgcat
    32941 cagggtaatc gccgcggttt caatggcaac atcactgccg ccacccatcg caatgccgac
    33001 atccgcctgg gccagcgctg gcgcgtcgtt aatgccgtcg cccaccattg ccacctgacg
    33061 tccttcactt tgcagacgtt tgatcgcttc ggctttaccg tccggcagca ctccggcaat
    33121 cacttcatca attccagctt ctttggcgat cgcattggcg gtggttgggt tatccccggt
    33181 caacatcacc agacgatatc ccgctttatg caggcgttgc agcgccgcca cgctatcact
    33241 acgcaacgga tcgcgtacag ccagcagggc tactgctttc ccgtcaatcg ccagcagcac
    33301 aggcgttgct ccttgcgatg cctgagctga aatatcggct tcaatcgctt tagtatcgac
    33361 ttgctgatca tttaacaacg cctgattacc cagcaataac gcatgacctt ctgcttcacc
    33421 gctcacgccc agcccgcgca atgtgcggaa accgttgacc tgcggtagct gcatatcgcc
    33481 tgctttatca aggatcgctc gtgccaatgg atggctggaa ccttgctcca gcgccgccgc
    33541 cagacgcagc gcctgcgctt cgtcaacatc agcaaatgtt ttcactgcga caacctgcgg
    33601 cttcccttcg gtcagcgtcc cggttttatc gaacacgacg gtatcgagtg tactggcgcg
    33661 ttgcagcgcg tcggcgtccc gcaccagcac gccaaactca gccgcccgcc cgacgccgga
    33721 aataatcgac atcggcgtcg ccagtcccag cgcacacggg caggcgataa tcagcaccgt
    33781 agtggcaatc accagggtat agacaatctg cggtgccgga ccaaagaaat accagattgc
    33841 cgcactgaca agcgcaataa ccactactac cggcacaaat atggctgaga ttttatccgc
    33901 cagctgaccg atttctggct tgctgctctg ggcctggcgc accatgcgaa tgatccttga
    33961 tagcgtggta tggctgccaa ccgcactggc acgaaacagc acactgccgt cctgtaccac
    34021 tgtcccggca tggacgctat cgccttcgcc tttttgctgc gggattggtt cgcccgtcag
    34081 catcgcttca tccagccatg cttcgccctg ggtaatctcg ccatctaccg gcacgcgatc
    34141 gccggtcgtc aggcgcagca acatacctgg ctgcacttct gccagaggca cgcttttttc
    34201 accttcgtca gtaaccaggc gcgccgtcgg tggggttaaa tcgagtaatt tctccagtgc
    34261 cttagaagaa cgctgacgtg cgcgtgcttc cagcatatgg ccgagattga tcagaccgat
    34321 aatcatcgca ctggcttcat agtaaagatg tcgcgcttcc atcgggaacc actgcggcca
    34381 caggttgacg ctcatcgaat agagccacgc cacgcccgtt cccagcgcca ccagcgtatc
    34441 catcgtcgct gcaccgttca gcaggctttt ccatgcactg cggtaaaaat ggccgctggc
    34501 gaaaaccatc accgccaggg ttatcaggcc gataaccaac cacaggctgc ggttgtcggc
    34561 ggtgactatc atgttatcgc cgatcatccc ccagaccatc accgggatac ccaccgccag
    34621 tgcgacaatt gcctgccagc ggaagcgctt catcgtagcg acggcggttt cttgctggcg
    34681 ttcgcggcgt ttcgcgtcat cttcaatcgc ttccgcgccg tagcccgctt tttccaccgc
    34741 ctgcactaaa tcttgtgggg aggcactgcc catcaccagc gcagtacgct ccgccaggtt
    34801 tacccgtgcc tgagtgacgc ccggtacgct ttgcagcgca ttttgtacgc gggtgacaca
    34861 gctggcgcag ctcatgccgc tcagcagcaa ctgctggctg tcatcgtcat cggcggtcgc
    34921 tgccggaagc gcctcagaaa ccgctgtcag tgcttccgac gggattgatg actccgccag
    34981 cggtttagcc tttgggtggc ttacagatgc gtcataaccc gcttgtttga tggtttcgat
    35041 cagctgttct gcactggcag tcccggtaac gtgcgcttca gtgatagaca catccgcttg
    35101 ttccacatcc gggcgctgtt caagactttc tttcacgcgt ttaacgcagt gaccgcagga
    35161 caggccgtcc aggttcaggt cgatagtttg tgacataaaa cactccttta agacagtttt
    35221 gactggctgt gataaaggtt aaaccttcca gcaaggggaa ggtcaagaaa ttaataaacc
    35281 aggcgggtaa aagtccgtaa agattaaaaa atcggctcga tttgcatcag gattagacat
    35341 ttatttcttt gttttcctgt agttaagttg cgggtgctaa gttaaatcag gatgcctgaa
    35401 aatcggcact ggggtgagga attacctccc gcatctataa aaaggagtta acaaaagatg
    35461 ttagatgcaa acaaattaca gcaggcagtg gatcaggctt acacccaatt tcactcactt
    35521 aacggcggac aaaatgccga ttacattccc tttctggcga atgtaccagg tcaactggcg
    35581 gcagtggcta tcgtgacctg cgatggcaac gtctatagtg cgggtgacag tgattaccgc
    35641 tttgcactgg aatccatctc taaagtctgt acgttagccc ttgcgttaga agatgtcggc
    35701 ccgcaggcgg tacaggacaa aattggcgct gacccgaccg gattgccctt taactcagtt
    35761 atcgccttag agttgcatgg cggcaaaccg ctttcgccac tggtaaatgc tggcgctatt
    35821 gccaccacca gcctgattaa cgctgaaaac gccgaacaac gctggcagcg aattttacat
    35881 atccaacagc aactggctgg tgagcaggta gcgctctctg acgaagtcaa ccagtcggaa
    35941 caaacaacca acttccataa tcgggccatt gcctggctgc tgtactccgc cggatatctc
    36001 tattgtgatg caatggaagc ctgtgacgtg tacacccgtc agtgctctac gctcatcaat
    36061 actgttgaac tggcaacgct tggcgcgacg ctggcggcgg gtggtgtgaa tccgttgacg
    36121 cataaacgcg ttcttcaggc cgacaacgtg ccgtacattc tggccgaaat gatgatggaa
    36181 gggctgtatg gtcgctccgg tgactgggcg tatcgcgttg gtttaccggg caaaagcggt
    36241 gtaggtggcg gtattctggc ggtcgtccct ggcgtgatgg gaattgccgc gttctcacca
    36301 ccgctggacg aagaaggcaa tagtgttcgc ggtcaaaaaa tggtggcatc ggtcgctaag
    36361 caactcggct ataacgtgtt taagggctga tcatgatgaa cacggaaggt aataacggta
    36421 acaaacccct cggtctatgg aacgtcgttt ccatcggtat tggggcaatg gtgggggcgg
    36481 ggatcttcgc gctgctgggg caggctgcgt tgctaatgga agcctcgacc tggggcgcct
    36541 ttgcttttgg cggtattgtg gctatgtttt ccggttatgc ctatgcgcgt ctgggggcga
    36601 gctatcccag caatggcggt attatcgact tttttcgtcg cggattaggc aacggcgtct
    36661 tttcgctagc gctctcgttg ctatacctgt tgacgctggc ggtgagcatc gccatggttg
    36721 cccgtgcttt tggcgcttat gccgtgcagt ttttgcatga aggcagccag gaggagcacc
    36781 ttattttgct ctacgcgttg gggatcattg cggtgatgac gcttttcaac tccttaagca
    36841 accatgcggt agggcggctg gaagtgatcc tcgtcggcat taaaatgatg atcctgttat
    36901 tgctgattat tgccggtgtc tggtcgctgc aaccggcgca tatttccgtc tctgcgcccc
    36961 ccagctccgg tgcgttcttc tcctgtattg ggataacctt ccttgcctat gcgggctttg
    37021 gcatgatggc gaacgcggcg gataaagtga aagatccgca ggtcattatg ccacgggcgt
    37081 ttctggtggc gattggcgtt accacgttgc tttatatctc gctggcgctg gttttgctta
    37141 gcgatgtatc ggcattagag ttagaaaaat atgctgatac cgccgtagcg caggctgctt
    37201 ctccgctgct cgggcatgtg ggttatgtga tcgtcgtcat cggcgcttta ctggcgacgg
    37261 cttcaggcat taacgcgaac ctgttcgccg tgtttaacat catggacaac atgggcagcg
    37321 aacgcgaact gccgaagcta atgaataaat ccctgtggcg gcagagtacc tggggcaata
    37381 tcatcgtcgt ggtgttgata atgctgatga cggcggcgct aaatttagac tcactcgcca
    37441 gcgttgccag cgccaccttt ttgatctgct atctggcggt gtttgtggtg gcgatccgcc
    37501 tgcgtcatga tattcacgcc tcgttgccga ttcttatcgt tggtacgttg gtgatgttgt
    37561 tggtgatcgt tggctttatc tacagcctgt ggtcccaggg cagccgtgcg ttgatatgga
    37621 ttattggtgc aatcttactc agccttattg tggcaatggt catgaagcgt aataaaaccg
    37681 tataacacct ctctgtgcgc agtacttcct gtattattgt ggtggcggtc gatattcgca
    37741 ctggcaaaaa aacgtgcttg aatatctgtt gaaacccttt aacaaagcac aggaggcgtt
    37801 gcgcgaacga tgaacatcag cgatgtagca aaaattaccg gcctgaccag caaagccatt
    37861 cgcttctatg aagagaaggg gctggtgacg ccgccgatgc gcagcgaaaa cggttatcgc
    37921 acctacacgc agcagcatct caacgaactg accttactgc gccaggcacg gcaggtgggc
    37981 tttaacctgg aagagagcgg cgagctggtg aatctgttta acgacccgca gcggcacagc
    38041 gccgacgtca aacggcgcac gctggagaag gtggcggaga tcgaacgaca cattgaggag
    38101 ctgcaatcca tgcgcgacca gctgctggca ctggcgaatg cctgccctgg cgatgacagc
    38161 gccgactgcc cgattatcga aaatctctcc ggctgctgtc atcatcgggc agggtgatta
    38221 agacgagacg gctcggatat gtagggttat cccttctatc gcaatgactt caacatgcgt
    38281 acctgcaccg agatcctcgc tggcgctgac aggccatgaa ctgtcaccga cgcgcatatg
    38341 accgcgcccg ttgaccagcg gagattccag cacaaaacgt cggccaatca gctgctgccc
    38401 gcgctggttt aaatgactat cgctgtgctt ttgttcgcgc acccgccgcg acaaccattt
    38461 ccaccacagc caggcggcga gcagcgtcag gatggcaaac attacccctt gccactccca
    38521 acccagcggc accagccaga ccaccaggcc ggtaatcacc gctgccacgc cgctccacaa
    38581 caaataacca tttccgccca gcatctcggc tgccagcagc aacccgccga gactgagcca
    38641 gaaaatatgt ggatgaacga ccattaactc catcatggct gagtccgttt gttggcgctg
    38701 tctttcacca gctcggcaat cccggcaatc gaccccatca ggctgctggc ctctaatggc
    38761 atcatcacta ctttgctgtt actggaggaa ccgatctgct gtaacgcttc ggtgtatttt
    38821 tgcgctacga agtagttcac cgcctgaata tcaccggagg cgatagcttc agacaccatt
    38881 ttggtggcgc gggcttctgc ttcggcggaa cgttcacgcg cttcagcctg taaaaacgcc
    38941 gactgacgtt cgccttccgc tttcaggatt tgcgactgtt tttcaccttc ggctttgagg
    39001 atttccgcct gacggatccc ttccgcttca agaatgtaag cgcgtttggt acgttccgct
    39061 ttcatctgcg cgttcattga agagataagc tctgccggtg ggcgcacgtc gcgaatttca
    39121 atacgggtga ctttaatccc ccacgggttg gtggcttcat cgacaatacg cagcaggcgt
    39181 gagttgatgc tgtcgcgctg agagagcatt tcgtcaagtt ccattgaacc caacacggta
    39241 cggatgttag tcatggtcag gttgatgatc gccagctcca gattgctgac ttcataggca
    39301 gcgcgtggcg cgtcaatcac ctgaataaaa cagactgcgt cgatggtaac gttggcgtta
    39361 tctttcgaga taacttcctg ggaagggata tcgagcactt gctccatcat attgatcttg
    39421 cgaccaatgc gatccataaa cggcaccacc agactgagcc ccggctgtaa cgttttggta
    39481 tagcgaccaa aacgttctac cgtccattga tagccctgcg gcacgatttt gacgcccgcg
    39541 ccgacaatga ccagcgcgac aaaaatgaga atcgggataa agataagcat cggaaaaacc
    39601 tcctgttgta ccgtccataa tcagcaaaat tgctgcttga ttaaacaaat tatacctgat
    39661 tactgaaaga gagttccccc ttattcctgc gaaggataaa ctgtttttag taaaaatcag
    39721 aaaaagggaa cagcgatgca ggaaaatagt cctttgcttc agctacaaaa cgtaggatat
    39781 ctggcgggtg atacgaagat tcttaataac atcaattttt cgctgcgtgc tggcgaattt
    39841 aagttaatta ccggtccttc tggttgtggc aaaagtacgc tgctaaaaat agttgcttca
    39901 ttgatcagcc caaccagcgg aacgttactg tttgaaggtg aggatgtcag cacactaaaa
    39961 ccagaaatct accgccagca agtctcttac tgcgcccaga cgccgacgct gtttggcgat
    40021 acggtatacg ataatctgat ctttccctgg cagatccgtg accagcagcc tgacccggcc
    40081 atttttctcg attttctcga acgcttcgct ttgccggaca gcattttgac gaagaatatc
    40141 gccgagctat ctggtggtga aaaacaacgc atctcattga ttcgtaacct gcaatttatg
    40201 ccgaaggttt tattgctgga tgaaataacc agtgcgctgg atgaaagtaa taaacataac
    40261 gtcaatgaga tgatccatcg ttatgtgcgc gagcaaaata ttgccgtgct gtgggtgaca
    40321 cacgataaag acgaaattaa tcatgcggat aaagtgatta cactgcaacc gcatgccgga
    40381 gaaatgcagg aagcacgcta tgaactcgca taatattact aacgaatcat tagcactggc
    40441 attaatgctg gtggtggtgg caatcttaat tagccataaa gaaaaactgg cgctggagaa
    40501 agatattcta tggagcgtcg ggcgagcgat aattcagctg attattgtcg gctatgtgct
    40561 gaagtatatt ttcagcgtgg atgatgccag cctgacatta ttgatggtgt tatttatctg
    40621 ctttaatgcg gcgtggaacg cgcaaaaacg cagtaaatat attgctaaag cttttatctc
    40681 atcgtttatt gccattacgg tcggggcggg aattaccctg gcggtgctga ttctctcagg
    40741 ggcgattgaa tttatcccga tgcaggtgat ccctgtcgcc gggatgattg ccggtaacgc
    40801 catggtagcg gtggggttgt gttacaacaa tttagggcaa cgggtcatta gcgaacagca
    40861 acagattcag gagaaactga gtcttggcgc gacgccgaag caggcttcag cgatattgat
    40921 tcgcgacagt attcgcgcgg ctttaattcc gacggtcgat tcagcaaaaa cggttggctt
    40981 agtgagttta ccaggaatga tgtccgggct gatatttgcc gggattgatc cggtgaaggc
    41041 gattaaatat cagattatgg tgacctttat gctgctctca accgccagct tgtcgaccat
    41101 tattgcctgc tatttaacct atcgtaagtt ttataattcg cgccaccagt tggtggtgac
    41161 tcaattgaag aagaaatgat gcgatgccgg atgcgcagca tcgcatccgg cgttgtggtt
    41221 catgtgccga acaacggaat atcaatacaa caatgcatac agctggcggc gatacttcga
    41281 cgccagtgca tcacccgtac ccagcgcagc gaggatctcc tggaacgttt tacgcgtctg
    41341 accgtctgcg gcggtgagat ctttacgcag atgcccgaac agcaactcca gcgcctcttc
    41401 attgcgccca acctgatgca gttgcagcgc cagttgcgtc gccagtgcgg catcttctgg
    41461 attctccgcc acctgctgtt gcaactgttg aatttccggc ttatcagccg cctgcttcag
    41521 cagttcgatt tgcgctacca gcccctggta gcgggtgtcc tgatcctgca acggaatggt
    41581 tttcagcacc gcttccgcat cttcagaacg gttcagcgca atcagcgttt ctgccagcag
    41641 caggccgatc tccccgttct gattcgacaa ctgccaggcg tctttcagca atggcagggc
    41701 atcggtgtaa ttgccttcct gcatcagttg catcgcctgc tgcgctttca gctcttcttc
    41761 gcgcggcagc actttatcca gcagggcgcg gatcgcctct tccggttgcg gcccctggaa
    41821 gccatctacc ggttgcccgt tctggaacag atacacggtc ggaatcgcgc gcagaccaaa
    41881 ctgggcggca atcatctgct ccgcgtcgca gtccagcttc gccagaataa attgcccgtt
    41941 gtactgcgcc gcgaggcttt ccaggatcgg ggttaactgc aaacagtgct ggctacgttc
    42001 agaccaaaaa tagaacagca ccggagtggt catcgactgt tcaagaacct gttgcaggtt
    42061 agattcgtta atgttgacaa tattttctac ggacatggag tcgctctctg ttgtcgatat
    42121 tttctttgac atgggggctt aagcgcgcgc ttcaactcac ccctgcaata ttttgtccat
    42181 cacgcgccca ggcagcaggc gctcgagcac cattactgcc caggtcacca gcgttaccgg
    42241 atagcgcatc ttcggcttct cgctaataaa agcatggcgc actttgtcga ccaccgcttc
    42301 cggtcccaac gtaaagcggg cggcgatgcc gggattttcg actggtttat cactttgcgt
    42361 ctggttgacg ttgtcggtga agcgagtacg aatgggaccg ggttcgatca ggctgacttt
    42421 aattccgctg tggcgtagct ccatgcgcag cgcgtctgac cacgcctcca gcgcatattt
    42481 actggccgcg taagcgccac gacccggcgt ggagattaat cccatcaccg atgatgtcat
    42541 cacaatacgc ccttcaccgt gcggtaacat cgcgggtaac aggcgcatgg tgagctggtg
    42601 tgcgccgaaa aagttggcgg aaaactgctg ttccatctgc gcacggctga tggtggaaag
    42661 ggggccatac atgccgaatc cggcattgtt aaagatccca tacagacaat tatcggtcag
    42721 ggcgatcacc tcgtctgctg cgcgattaac actttctggt gaatccagat cgatcaacac
    42781 gccggtaaat cccatgttgt tcatacgctc aacatcattc ggttttcggc aacctgccag
    42841 cacatgaaaa ccctggcgtt ttaattcgag cgcgctttcc aggccaattc cactggaaca
    42901 tccggtaatt aagaccgatt tttgcataac tttacctgtc aggatctccg ttgctttatg
    42961 agtcatgatt tactaaaggc tgcaactgct tcgccatcca gtcggcaata aacggctggg
    43021 cgtcgcggtt gggatgaata ccgtcatcct gcatccattg tggcttgagg tagacctctt
    43081 ccataaaaaa gggcagcagc ggaacatcaa actctttggc gagtttgggg taaatggcgc
    43141 taaaggcttc attataacgg cgaccatagt ttgcaggcag acgtatttgc attaacaatg
    43201 gttcagcgtt ggcggctttg acatcctgca aaatctggcg cagcgtttgc tcggtttgct
    43261 gtggctgaaa accacgcaaa ccatcattgc gcccagttca accagcaccc aacgcggctg
    43321 atgctgtttg agcagagccg gaaggcgcgc cagcccttgt tgcgaggtgt cgccgctgat
    43381 gctggcatta actaccgacg ttttactctg ccacttatca ttcaacaagg caggccaggc
    43441 cgcgctggca gacattcgat acccggcgct caggctatca cccagaatca ataacgtgtc
    43501 cgctgcggcg gcacggaagg ttaacaggac caggaacagg aagggcaaat gccagcggaa
    43561 aacattgttg aagttcatca tcttaagaag tccgtcggtc agggggagca tgaactctcc
    43621 atcctcaccg gagttgagct ggttgtcaaa cgtggcgaga ccatcgcact ggtgggcgag
    43681 tcgggatcgg gtaagtcaac cttgctggcg atcctcgccg ggcttgatga cggcagcagt
    43741 ggcgaagtga gtctggtggg acaaccgcta cataatatgg acgaagaagc gcgggcaaag
    43801 ttgcgcgcga agcacgtcgg ctttgttttt cagttattta tgttaattcc tacccttaac
    43861 gcgctggaaa acgtcgagct tccggctctg ctgcgcggtg agagtagcgc ggaaagtcgt
    43921 aacggggcga aagcgttgct cgaacagtta gggctgggta aacgtctgga tcatcttccg
    43981 gcacagcttt ccggcggtga acagcaacga gtggcgctgg cacgagcctt taatggtcga
    44041 cctgatgtgc tgtttgccga cgaacccacc ggcaaccttg accgccagac gggcgataaa
    44101 attgccgacc tgctgttttc cctcaaccgt gaacatggca ccacgttgat tatggtgacc
    44161 cacgacctgc aactggcggc acgctgcgac cgctgcttac ggctggtgaa cgggcagttg
    44221 caggaggaag catgattgca cgttggttct ggcgcgaatg gcgttcgccg tcgctattaa
    44281 ttgtctggct ggcgctaagc ctggcggtgg cctgcgtgct ggcgctgggc aatatcagcg
    44341 atcgcatgga gaagggctta agccagcaaa gccgtgagtt tatggcgggc gatcgggcgt
    44401 tgcgcagttc acgcgaagtg ccgcaagcgt ggctggagga agcgcaaaag cgcggcctga
    44461 aagtcggcaa gcagctgact ttcgccacaa tgacctttgc aggcgacaca ccgcagctgg
    44521 cgaacgtcaa agcggtggat gatatctacc cgatgtatgg cggtctgcaa actaatcccc
    44581 ctggcctgaa accgcaggcg ggcagcgtat tgctggcccc acgcctgatg gcactgctta
    44641 acctgaaaac gggcgacacc attgacgtgg gcgatgccac cttgcggatt gccggagaag
    44701 tgattcagga accggattcc ggttttaacc ccttccagat ggctccgcgt ctgatgatga
    44761 atctggcgga tgtcgataaa accggagccg tgcagccggg gagtcgggtc acctggcgtt
    44821 ataaattcgg cggcaacgag aaccagctcg acggctatga gaaatggttg ttacctcagc
    44881 ttaaacccga acaacgctgg tacggtctgg aacaggacga aggcgcgctg gggcgatcga
    44941 tggaacgctc gcaacagttc ctgctgcttt cggcgcttct gaccttgctg ctggcagtgg
    45001 cagcggtggc ggtagcgatg aatcattact gtcgcagtcg ctacgatctg gtggcgatcc
    45061 tcaaaacgct gggggcaggg cgagcgcaac tgcgtaagct aatcgtcggt cagtggttga
    45121 tggtgctgac gctttcagcc gttaccggtg gggccatagg cctgttgttc gaaaacgtgt
    45181 tgatggtgct gctcaagccc gttctgcctg ctgcactacc gccagccagc ctctggccgt
    45241 ggctgtgggc gcttggcacc atgacggtca tctcgctgct ggtggggcta cgaccatacc
    45301 gcttgttgct ggcaacgcag cctttacgcg tattacgtaa tgatgtggtc gcgaacgtct
    45361 ggccgctgaa gttttatctg ccgattgtca gtgtggtggt tgtgctgctg ctcgccggat
    45421 taatgggtgg cagcatgctg ctttgggcgg tgctggcggg cgcggtagta ctggctttgc
    45481 tgtgcggtgt gctgggctgg atgctgctga atgtacttcg ccgcatgacg ctgaaatcgc
    45541 tgcctctgcg cctggcggtt agccgcctgt tacgtcagcc gtggtcaacg ttaagccagc
    45601 tttcggcatt ttcgctctcc tttatgctgc tggcactgct gctggtgctg cgtggcgatc
    45661 tgctcgaccg ctggcagcag cagctaccgc cagaaagccc gaactacttt ttaattaaca
    45721 tcgccacaga acaggtagca ccgctaaaag cgttcctcgc ggaacatcat atagtcccgg
    45781 aatcatttta tccggtagtg cgggcgcggc tgacggcgat taatgataag ccgacagaag
    45841 gtaatgaaga tgaggcgctt aatcgcgaac tcaatcttac ctggcaaaat acgcggcccg
    45901 atcataatcc gattgtcgcc ggtaactggc cgccaaaagc cgatgaagtg tcgatggaag
    45961 aggggctggc gaaacgctta aacgttgccc tcggtgatac cgtgactttt atgggcgata
    46021 cccaggagtt ccgcgctaaa gtgaccagcc tgcgcaaagt ggactgggaa agtctgcggc
    46081 ctaatttcta ttttattttc cctgaagggg cattagacgg gcaaccgcag agctggctta
    46141 ccagtttccg ctgggagaat ggcaacggca tgttgacgca actcaaccgc cagttcccga
    46201 ccattagcct gttagacatt ggcgcgattt taaaacaggt cggtcaggtg ctggagcagg
    46261 taagtcgggc gctggaggtg atggtggtgc tggtcaccgc ctgcggtatg ttgctgttgc
    46321 tggcacaggt gcaggtggga atgcgccagc gtcatcagga gctggtggta tggcgcacac
    46381 tcggtgcggg gaaaaaactg ctgcgcacga cattgtggtg tgatgttcgc catgcttggg
    46441 tttgtttccg gcctggtggc cgcaattggt gcggaaacgg cactggcagt gttgcaggcg
    46501 aaagtgtttg atttcccgtg ggagccagac tggcgattgt ggattgttct gccgtgcagc
    46561 ggtgcgctgc tgctgtcgct tttcggcggc tggctgggtg cgcgactggt taagggtaag
    46621 gcgctgttca ggcagtttgt ggggtgatga caggtgttag attgcctgat gcgctacgct
    46681 tatcaggcct acgaagagcg tgcaatatat tgattttgca agattgtgta ggccggataa
    46741 ggcgtttacg ccgcatctgg catgaacagt gtgcattttg gcaataaata tgaaaccaac
    46801 tgtcatgtcg gtttttttaa aggccgaagg aggtaaacct tcggccttag ctacgtttcc
    46861 tcagttattc aataaactga cggatcagtt ccagcagcgc gattagcgcc ttaagaacga
    46921 tgagaacttt atcgagaaaa gttttcatcc gttgctccct taagggagaa agacaatcac
    46981 agcttaccct tacgttgcct ataactatga ttttcttcat gtgctagatg aggactgtct
    47041 ctctccaaat tagagcacca gaatcagcac cacctgtatc ctgaccagga cttaaaagta
    47101 cttttccgca ctgcggtaat cccttgaggc agtatcacta ctagcgtcgg gattgtatcg
    47161 ccaacatagc tgtttgaata tgtaggcatc ctgacttata attcacttgc tagatgagga
    47221 ctgtttaatt tctgcccgga accaccaagt cttgggcgga aaaaataaaa aaaccgactg
    47281 tcatgtcggt ttttttatat ctgtattctg gtgcaaccat tttgttctca cgacaatcgc
    47341 cgtttcagaa taccgcccat cactgcgcct taacccatac cgccacttcc gcccactcac
    47401 cgcgaaagac aactttttcc gcttttttct caagctgata gcgatacatt gggtcgtaat
    47461 attctttaag taacggcacc agccaggcca gatgaccgtc ggtgctgccg gtggtgagtt
    47521 gcgttgtcag cgccgtatcc agctgggcag cgagttcgtt atagcgctgt aaccccagcc
    47581 gacgcttaat cgccgaaagt ccgtgatgca ggtattcgca atattcctgc cagccctgtt
    47641 cgtcgccata agcgtgggta aaatcatgat gcatacgcaa gaaatactct tcattcaggc
    47701 gttcaagacg gatctcaaac ggatcttcta ccaccgcaat cgccgcctga gtcattcgct
    47761 cgcgcagaca ttccggcagg tgattcgaac cgatcatccg gctttcatct tccagcaccc
    47821 acaggcgcaa atcctgacgg gcgtcggttt ttagcatttc ggcagccagc aggttttcaa
    47881 aactcgcctg gctaagttgt ggttgtaacg tgcgaccaaa cgccgaaccg cgatgacgcg
    47941 ccagcccttc cagatcaaca ccgtttggct gttgttgcac taatagcgtt ttaccgctgc
    48001 cggtacaacc gccaatcagc actatcggtt tttgtgccag ttcaatggtc gcctgaatcg
    48061 cggtctggcg cagtgcctta taaccgcctt ccaccagcgg ataatcaatc cccgcttcat
    48121 gcagccagcg ttgcacaata tgtgaacgtt gaccgccacg ggcgcagcag agaatacctt
    48181 gcggattttg caggcacgct gcccgccagg cgtacatacg ctgctgacga atttcacccg
    48241 ccaccagttt atgccccagc gccagcgctg cgtctgagcc ttgctgttta tagcaggtgc
    48301 caacggaggc gcgttcatcg ttatccatta acggcagatt gatagcggcg ggcattgcgc
    48361 cttgctcaaa ctcgataggg gcgcgaacat caataatggg cgtatcagca atcagcaggg
    48421 cacgatagtc ctgttccgtg tgtctctctt gcatagttaa aagtgaacct caaatcagct
    48481 tgcgcgctat tttacgcgcc aacgcgcaag gaaacttgat ttttaactgc gtggggtgcc
    48541 gaaaatttct aaaaatccgc tgatttccgg cctgtgttgg gtaaacaagg tcacaatatc
    48601 ttctaccgct ttgcagctgc caaatttgcg ccatgccaga ctcaatggcg aaggagggcg
    48661 catcgttggg attacccggc tgaccagttg ttgattatcg atcattgact ggcaaagtga
    48721 ttttggcaaa aaaccaatgc caacacccgc cagatgggcg gcgattttcg tttccatatc
    48781 gggaacaata atctcttttt gccccggcaa tcgccaggcg acgcgtttgg ttaaggtgcc
    48841 ggcgctgtct tcaatattga ccgccggaaa gcgacgcaac tgcgcttctg ttagcggctc
    48901 ttcaacgttc gccagcggat gatccgccgc catgacaaat cgccattgca ccgatcctaa
    48961 gggatcaaga ctaaaggtat ttgccagcgc ctcagttccc gtgaccccga tagccagcga
    49021 aaaaccttcg tacaatagcg agtcccagac gcccatatag atttgtcggg agatgtgaaa
    49081 ctgggtaaag gggtaacgct cattcagcca cgccagcaac tgggcgacgg cctgggggtt
    49141 atagagcagg ttgttgatga caatattcac ctggcgttcc acgccatcat tcacctgttg
    49201 cagctcgctg ggcatacttt ccagccagct cagccagtct ctggcctggg aaagtagatg
    49261 ctcgccagcc gctgtcaacg ttacgctacg agtcgtacgg aaaaacagcg ctactccggt
    49321 attctcttcc agaagtttaa tgcgataact gatcgtcgcc gtggttttac ataatcgttc
    49381 tgccgctttt gaaaaacttc ctgtttcagc aaccgcaatg aaagtccgca aggtttctgg
    49441 atcgaacatc ttcaggtatc cccttttaaa tccgcaagtt gcgtgatttt cttatcctct
    49501 gatttatcag tatttttaca tgataatcct gttcaatttg tggactaaat ctagttttgg
    49561 aaaaacattc caacttttgt attaatgttg ttctcttaag gttttagatt gcctgttatt
    49621 gaaaccaagc tgaccggtcg gcggtggttg aacggaatta tgttacaagg acaaaaagat
    49681 gaaacttcag gtattaccgt taagtcagga agcctttagt gcttatggcg acgtaatcga
    49741 aacgcagcaa cgggattttt tccatattaa caatggcctg gtggagcgtt accacgattt
    49801 ggcgctggtt gagattcttg agcaagaccg tacgcttatc agcattaacc gcgcgcaacc
    49861 ggcgaatctg ccgctgacca ttcacgaact cgaacgtcat ccgctgggca ttcaggcctt
    49921 tatcccaatg aaaggtgaag tgtttgtggt ggtcgtggcg ttaggcgacg acaaaccaga
    49981 cctgtcaacg ctgcgggcgt ttatcaccaa cggtgaacag ggggtgaatt accatcgtaa
    50041 cgtctggcat cacccacttt tcgcctggca gcgcgtcacc gattttctga ccatcgatcg
    50101 cggcggcagt gacaactgtg atgttgaaag tattcctgaa caggaactct gttttgcgtg
    50161 acgcctgcaa ccgacttgca taagataaac taattgttca ttgtttatgc tcacttgtag
    50221 gtcggagtta acgtaggtat gacggaagtt agacggcgcg gcaggccagg acaggcggag
    50281 cctgtggcac agaagggcgc acaggcgtta gagcggggaa ttgcgattct gcaatatctg
    50341 gaaaaaagtg ggggaagttc gtcggttagc gatatttctc tcaatctgga tttgccgctc
    50401 tccacgacct ttcgcttgct gaaggtttta caagcagcgg attttgtcta tcaggacagt
    50461 caattaggct ggtggcatat aggattaggt gtctttaacg tcggtgcggc gtacatccat
    50521 aaccgcgatg tcctctccgt cgccgggccg tttatgcgcc gcctgatgtt actttccggc
    50581 gaaacggtca atgtcgcgat ccgtaacggc aatgaagcgg tattaattgg tcagttagag
    50641 tgtaaatcga tggtcaggat gtgtgcgcca ctgggcagtc gtctgccgct gcatgcttcc
    50701 ggtgcgggca aagcgctgct ttatccgctg gcggaagagg agttgatgag catcattctg
    50761 caaaccggtt tgcagcagtt tacgccaact acgcttgtgg atatgcccac cttgctgaag
    50821 gacctggaac aagcgcgtga actgggctat accgtagata aagaagagca tgttgtaggt
    50881 ctgaattgca tagcttcagc aatttacgat gatgtcggta gagttgttgc cgctatctcc
    50941 atctccgggc cttcatcaag actgacagaa gatcgttttg tcagtcaggg tgagctggtc
    51001 agagacaccg cccgcgatat cagcacggcg ttgggactga aagcacatcc atagtgtctg
    51061 tcgcatcccg ctctgcggag cgggtttttt tgacaaaatt tgaaagttgg aaaatttttc
    51121 caactaatag aggtaggaat aaaatggcaa aaatgagagc cgttgacgcg gcaatgtatg
    51181 tgctggagaa agaaggtatc accaccgcct tcggcgttcc gggagctgca attaatccgt
    51241 tctactcagc gatgcgtaag cacggcggta ttcgtcacat tctggcgcgt catgtggaag
    51301 gtgtttcgca catggcggaa ggttataccc gcgcaacggc agggaatatc ggcgtatgtc
    51361 tggggacttc cggtcctgcg ggcacggaca tgatcaccgc gctctattcc gcttctgctg
    51421 attccattcc tattctgtgc attaccggcc aggcaccgcg cgcccgtctg cataaagaag
    51481 attttcaggc cgtagatatt gaagcaattg ctaaaccggt cagcaaaatg gcggttacag
    51541 ttcgtgaagc ggcgctggtg cctcgcgtgc tgcaacaggc atttcacctg atgcgttctg
    51601 gtcgtccggg tccggtactg gtggatttac cgttcgacgt tcaggttgcg gaaatcgagt
    51661 ttgatcctga catgtacgaa ccgctgccgg tctacaaacc tgctgccagc cgtatgcaga
    51721 tcgaaaaagc tgtagaaatg ttaatccagg ccgaacgtcc ggtgattgtt gccgggggcg
    51781 gggtaatcaa tgctgacgca gctgcactgt tacaacagtt tgctgaactg accagcgttc
    51841 cggtgatccc aacgctgatg ggctggggct gtatcccgga cgatcatgaa ctgatggccg
    51901 ggatggtcgg tctgcaaacc gcgcatcgtt acggtaacgc aacgttgctg gcgtccgaca
    51961 tggtgtttgg tatcggtaac cgttttgcta accgtcatac cggttcggta gagaaataca
    52021 ccgaagggcg caaaatcgtt catattgata ttgagccgac gcaaattggt cgcgtgctgt
    52081 gtccggatct cggcattgtc tctgatgcta aagcggcgct gacactgctg gttgaagtgg
    52141 cgcaggagat gcaaaaagcg ggtcgtctgc cgtgtcgtaa agaatgggtc gccgactgcc
    52201 agcagcgtaa acgcactttg ctgcgcaaaa cccacttcga caacgtgccg gtgaaaccgc
    52261 agcgcgtgta tgaagagatg aacaaagcct ttggtcgcga tgtttgttat gtcaccacta
    52321 ttggtctgtc acaaatcgct gcggcacaaa tgctgcatgt ctttaaagac cgccactgga
    52381 tcaactgtgg tcaggctggt ccgttaggct ggacgattcc ggcggcgctg gggggttgtg
    52441 ccgctgatcc gaaacgcaat gtggtggcga tttctggcga ctttgacttc cagttcctga
    52501 ttgaagagtt agccgttggc gcgcagttca acattccgta catccatgtg ctggtgaata
    52561 acgcctatct cggcctgatt cgtcagtcac aacgcgcttt tgacatggac tactgcgtgc
    52621 aactcgcttt cgagaatatc aactccagtg aagtgaatgg ctacggtgtt gaccacgtaa
    52681 aagtagcgga aggtttaggt tgtaaagcta ttcgggtctt caaaccggaa gatattgcgc
    52741 cagcctttga acaggcgaaa gccttaatgg cgcaatatcg ggtaccggta gtcgtggaag
    52801 ttattctcga gcgtgtgacc aatatttcga tgggcagcga actggataac gtcatggaat
    52861 ttgaagatat cgccgataac gcagcggatg caccgactga aacctgcttc atgcactatg
    52921 aataagggag ataaatgatg ttacgtttct ctgctaattt atcgatgtta tttggtgaat
    52981 atgattttct cgcccgtttt gagaaagctg cgcagtgtgg ttttcgcggc gttgaattta
    53041 tgtttcctta tgactacgac attgaagaat taaaacaggt actggcgagt aataaactcg
    53101 aacatacgct gcacaattta ccggcaggtg actgggcggc gggcgagcgc ggtattgcct
    53161 gtattcctgg ccgtgaagaa gagtttcggg atggcgtagc agcagcgatt
//
LOCUS       DAZPCS010000014        48333 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZPCS010000014 DAZPCS010000000
VERSION     DAZPCS010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48333
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.014"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            82..486
                     /locus_tag="ACHUQ7_000764"
     CDS             82..486
                     /locus_tag="ACHUQ7_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFR1134297.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            512..1225
                     /gene="msyB"
                     /locus_tag="ACHUQ7_000765"
     CDS             512..1225
                     /gene="msyB"
                     /locus_tag="ACHUQ7_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFR1134298.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSILM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSTYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            1374..1940
                     /gene="satP"
                     /locus_tag="ACHUQ7_000766"
     CDS             1374..1940
                     /gene="satP"
                     /locus_tag="ACHUQ7_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFR1134299.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(1975..2562)
                     /gene="mog"
                     /locus_tag="ACHUQ7_000767"
     CDS             complement(1975..2562)
                     /gene="mog"
                     /locus_tag="ACHUQ7_000767"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFR1134300.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(2677..3630)
                     /gene="tal"
                     /locus_tag="ACHUQ7_000768"
     CDS             complement(2677..3630)
                     /gene="tal"
                     /locus_tag="ACHUQ7_000768"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFR1134301.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            3909..5339
                     /locus_tag="ACHUQ7_000769"
                     /pseudo
     CDS             3909..5339
                     /locus_tag="ACHUQ7_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
     gene            5409..6185
                     /gene="yaaA"
                     /locus_tag="ACHUQ7_000770"
     CDS             5409..6185
                     /gene="yaaA"
                     /locus_tag="ACHUQ7_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFR1134302.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(6338..6634)
                     /locus_tag="ACHUQ7_000771"
     CDS             complement(6338..6634)
                     /locus_tag="ACHUQ7_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFR1134303.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(6848..8134)
                     /gene="thrC"
                     /locus_tag="ACHUQ7_000772"
     CDS             complement(6848..8134)
                     /gene="thrC"
                     /locus_tag="ACHUQ7_000772"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFR1134304.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFSALRKLMMNHQ"
     gene            complement(8135..9067)
                     /gene="thrB"
                     /locus_tag="ACHUQ7_000773"
     CDS             complement(8135..9067)
                     /gene="thrB"
                     /locus_tag="ACHUQ7_000773"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFR1134305.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNKLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(9069..11531)
                     /gene="thrA"
                     /locus_tag="ACHUQ7_000774"
     CDS             complement(9069..11531)
                     /gene="thrA"
                     /locus_tag="ACHUQ7_000774"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFR1134306.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(11557..11676)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(11612..11677)
                     /gene="thrL"
                     /locus_tag="ACHUQ7_000775"
     CDS             complement(11612..11677)
                     /gene="thrL"
                     /locus_tag="ACHUQ7_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFR1134307.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(11891..12577)
                     /locus_tag="ACHUQ7_000776"
     CDS             complement(11891..12577)
                     /locus_tag="ACHUQ7_000776"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFR1134308.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(12977..13117)
                     /gene="yjjY"
                     /locus_tag="ACHUQ7_000777"
     CDS             complement(12977..13117)
                     /gene="yjjY"
                     /locus_tag="ACHUQ7_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFR1134309.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            13213..13929
                     /gene="arcA"
                     /locus_tag="ACHUQ7_000778"
     CDS             13213..13929
                     /gene="arcA"
                     /locus_tag="ACHUQ7_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFR1134310.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(13988..15340)
                     /gene="creD"
                     /locus_tag="ACHUQ7_000779"
     CDS             complement(13988..15340)
                     /gene="creD"
                     /locus_tag="ACHUQ7_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFR1134311.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(15398..16822)
                     /gene="creC"
                     /locus_tag="ACHUQ7_000780"
     CDS             complement(15398..16822)
                     /gene="creC"
                     /locus_tag="ACHUQ7_000780"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFR1134312.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMGGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLDQALGNLLDNAIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(16822..17511)
                     /gene="creB"
                     /locus_tag="ACHUQ7_000781"
     CDS             complement(16822..17511)
                     /gene="creB"
                     /locus_tag="ACHUQ7_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFR1134313.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALYPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(17524..17997)
                     /gene="creA"
                     /locus_tag="ACHUQ7_000782"
     CDS             complement(17524..17997)
                     /gene="creA"
                     /locus_tag="ACHUQ7_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFR1134314.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            18208..19077
                     /gene="robA"
                     /locus_tag="ACHUQ7_000783"
     CDS             18208..19077
                     /gene="robA"
                     /locus_tag="ACHUQ7_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFR1134315.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(19074..19721)
                     /gene="gpmB"
                     /locus_tag="ACHUQ7_000784"
     CDS             complement(19074..19721)
                     /gene="gpmB"
                     /locus_tag="ACHUQ7_000784"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFR1134316.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            19773..20293
                     /gene="yjjX"
                     /locus_tag="ACHUQ7_000785"
                     /pseudo
     CDS             19773..20293
                     /gene="yjjX"
                     /locus_tag="ACHUQ7_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
     gene            complement(20378..20704)
                     /gene="trpR"
                     /locus_tag="ACHUQ7_000786"
     CDS             complement(20378..20704)
                     /gene="trpR"
                     /locus_tag="ACHUQ7_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFR1134317.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(20794..22731)
                     /gene="sltY"
                     /locus_tag="ACHUQ7_000787"
     CDS             complement(20794..22731)
                     /gene="sltY"
                     /locus_tag="ACHUQ7_000787"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFR1134318.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLIQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            22942..24609
                     /gene="ettA"
                     /locus_tag="ACHUQ7_000788"
     CDS             22942..24609
                     /gene="ettA"
                     /locus_tag="ACHUQ7_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFR1134319.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(24716..24883)
                     /gene="nadS"
                     /locus_tag="ACHUQ7_000789"
     CDS             complement(24716..24883)
                     /gene="nadS"
                     /locus_tag="ACHUQ7_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFR1134320.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(24917..26149)
                     /gene="nadR"
                     /locus_tag="ACHUQ7_000790"
     CDS             complement(24917..26149)
                     /gene="nadR"
                     /locus_tag="ACHUQ7_000790"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFR1134321.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(26170..27552)
                     /gene="radA"
                     /locus_tag="ACHUQ7_000791"
     CDS             complement(26170..27552)
                     /gene="radA"
                     /locus_tag="ACHUQ7_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFR1134322.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(27601..28569)
                     /gene="serB"
                     /locus_tag="ACHUQ7_000792"
     CDS             complement(27601..28569)
                     /gene="serB"
                     /locus_tag="ACHUQ7_000792"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFR1134323.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            28675..29319
                     /locus_tag="ACHUQ7_000793"
     CDS             28675..29319
                     /locus_tag="ACHUQ7_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFR1134324.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            29347..30362
                     /gene="lplA"
                     /locus_tag="ACHUQ7_000794"
                     /pseudo
     CDS             29347..30362
                     /gene="lplA"
                     /locus_tag="ACHUQ7_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            30394..30657
                     /locus_tag="ACHUQ7_000795"
     CDS             30394..30657
                     /locus_tag="ACHUQ7_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR1134325.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(30818..31537)
                     /gene="deoD"
                     /locus_tag="ACHUQ7_000796"
     CDS             complement(30818..31537)
                     /gene="deoD"
                     /locus_tag="ACHUQ7_000796"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFR1134326.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(31594..32817)
                     /gene="deoB"
                     /locus_tag="ACHUQ7_000797"
     CDS             complement(31594..32817)
                     /gene="deoB"
                     /locus_tag="ACHUQ7_000797"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFR1134327.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(32869..34191)
                     /gene="deoA"
                     /locus_tag="ACHUQ7_000798"
     CDS             complement(32869..34191)
                     /gene="deoA"
                     /locus_tag="ACHUQ7_000798"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFR1134328.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADNAPESTPTVYRRISE"
     gene            complement(34318..35097)
                     /gene="deoC"
                     /locus_tag="ACHUQ7_000799"
     CDS             complement(34318..35097)
                     /gene="deoC"
                     /locus_tag="ACHUQ7_000799"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFR1134329.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGDEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            35355..36896
                     /locus_tag="ACHUQ7_000800"
     CDS             35355..36896
                     /locus_tag="ACHUQ7_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFR1134330.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSQ"
     gene            36868..37731
                     /locus_tag="ACHUQ7_000801"
     CDS             36868..37731
                     /locus_tag="ACHUQ7_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFR1134331.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(37945..38727)
                     /locus_tag="ACHUQ7_000802"
     CDS             complement(37945..38727)
                     /locus_tag="ACHUQ7_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR1134332.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAVLGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(38724..39797)
                     /locus_tag="ACHUQ7_000803"
     CDS             complement(38724..39797)
                     /locus_tag="ACHUQ7_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFR1134333.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRISEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(39919..40080)
                     /locus_tag="ACHUQ7_000804"
     CDS             complement(39919..40080)
                     /locus_tag="ACHUQ7_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFR1134334.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(40197..40802)
                     /gene="osmY"
                     /locus_tag="ACHUQ7_000805"
     CDS             complement(40197..40802)
                     /gene="osmY"
                     /locus_tag="ACHUQ7_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFR1134335.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(41195..42784)
                     /gene="prfC"
                     /locus_tag="ACHUQ7_000806"
     CDS             complement(41195..42784)
                     /gene="prfC"
                     /locus_tag="ACHUQ7_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFR1134336.1"
                     /translation="MTLSPYLKEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLNGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(42875..43552)
                     /gene="yjjG"
                     /locus_tag="ACHUQ7_000807"
     CDS             complement(42875..43552)
                     /gene="yjjG"
                     /locus_tag="ACHUQ7_000807"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFR1134337.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRSNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(43567..44013)
                     /gene="rimI"
                     /locus_tag="ACHUQ7_000808"
     CDS             complement(43567..44013)
                     /gene="rimI"
                     /locus_tag="ACHUQ7_000808"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFR1134338.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(43982..44395)
                     /gene="holD"
                     /locus_tag="ACHUQ7_000809"
     CDS             complement(43982..44395)
                     /gene="holD"
                     /locus_tag="ACHUQ7_000809"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFR1134339.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            44498..45529
                     /gene="rsmC"
                     /locus_tag="ACHUQ7_000810"
     CDS             44498..45529
                     /gene="rsmC"
                     /locus_tag="ACHUQ7_000810"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFR1134340.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGCFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            45806..45892
                     /locus_tag="ACHUQ7_000811"
     tRNA            45806..45892
                     /locus_tag="ACHUQ7_000811"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45840..45842,aa:Leu,seq:cag)
     gene            complement(45931..46167)
                     /locus_tag="ACHUQ7_000812"
     CDS             complement(45931..46167)
                     /locus_tag="ACHUQ7_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFR1134341.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
     gene            46308..47096
                     /gene="fhuF"
                     /locus_tag="ACHUQ7_000813"
     CDS             46308..47096
                     /gene="fhuF"
                     /locus_tag="ACHUQ7_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFR1134342.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            complement(47134..47811)
                     /gene="bglJ"
                     /locus_tag="ACHUQ7_000814"
     CDS             complement(47134..47811)
                     /gene="bglJ"
                     /locus_tag="ACHUQ7_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFR1134343.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIVDDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAEDILLCMRHCEA
                     SNVLHPY"
     gene            complement(47769..>48333)
                     /locus_tag="ACHUQ7_000815"
     CDS             complement(47769..>48333)
                     /locus_tag="ACHUQ7_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1134344.1"
                     /translation="VIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAV
                     ELLMCPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTL
                     SERKVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGA
                     RELNLPSVYGDAAEWNTAELRREMSHS"
BASE COUNT        11471 a        13129 c        12141 g        11592 t
ORIGIN      
        1 ttacgtgcct gaatcttctc tttatcagca gtaaactagt gggtattcat ccccctacct
       61 cttcccacta agagaatcct tatgaaatcc gtttttacga tttccgccag cctggcgatt
      121 agcctgatgc tgtgctgcac ggcgcaggca aacgaccata aactcctcgg cgtcattgca
      181 atgccgcgta acgaaaccaa cgatctggcg ctgaaacttc ctgtttgtcg cattgtgaaa
      241 cgcatacaac tctctgccga ccatggcgat ttacagttaa gtggtgcatc ggtttatttc
      301 aaagccgccc gtagcgccag tcagagcctg aatattcctt cagaaataaa agaagggcaa
      361 accactgact ggatcaacat taacagcgat aacgacaata aacgctgcgt ctcaaaaatc
      421 accttttccg gtcatacggt gaactcatcg gatatggcca cgctgaaaat tatcggcgac
      481 gactaacgcc agaatataga agccacaaaa aatgaatgtt aattacctga atgattcaga
      541 tctggatttt cttcagcatt gtagtgagga acagttggca aatttcgccc gattgctcac
      601 ccataatgaa aaaggcaaaa ctcgcctctc cagcatactg atgcgcaacg aactgtttaa
      661 atcgatggaa gggcatcccg agcaacatcg ccgcaactgg cagctgattg ccggagaatt
      721 acagcatttt ggtggcgata gtatcgccaa caaactgcgc ggacacggta aattgtatcg
      781 ggccattttg ctcgatgttt caaagcgatt gaagctgaaa gccgacaaag agatgtctac
      841 gtttgaaatt gagcaacagt tactggaaca atttctgcgt aatacctgga agaaaatgga
      901 cgaggaacat aagcaggagt ttctgcacgc ggtcgatgcc agggtgaatg agctggaaga
      961 gctgctgccg ctgctgatga aagacaaatt attggcaaaa ggtgtgtcgc atttgctttc
     1021 cagccaactg acccgcattt tacgcaccca cgcagcaatg agcgtacttg ggcatggttt
     1081 gctgcgcggc gcggggctgg gaggccctgt gggcgcagca ttaaatgggg ttaaagcggt
     1141 cagcggcagc acctatcgcg tgacgattcc agccgtactg caaatcgcct gcctgcgccg
     1201 gatggttagc gccactcagg tctgataccg gaaaccgggg aaaattttca ttttcccctc
     1261 aaaagatcgt agacactgcc ccactggctg attattatgc cgcgccctga aaacactaca
     1321 gttattcagg gaaattattt caccattcat tcgatgatga tttttgagga attatgggca
     1381 acactaagtt ggctaatccg gcaccgctgg gcctgatggg cttcggcatg accaccattc
     1441 tgcttaacct gcacaacgtg ggttatttcg ctctggacgg tattattctt gccatgggca
     1501 ttttctacgg cggcatcgcg caaatttttg ctggtctgct ggagtacaaa aaaggcaaca
     1561 ctttcggttt aaccgcattc acctcttacg gttctttctg gctgacgctg gttgcgattc
     1621 tgctgatgcc gaaactgggt ctgaccgatg cgccaaatgc acagttcctt ggtgtctacc
     1681 tgggtctgtg gggcgtattt acgctgttta tgttcttcgg cactctgaaa ggcgcgcgcg
     1741 tcctgcaatt cgttttcttt agcctgaccg tgctgtttgc cctgctggcg atcggtaaca
     1801 ttgccggtaa tgccgcgatc atccactttg ccggctggat tggtctgatc tgcggtgcca
     1861 gcgcaatcta tctggcgatg ggtgaagtgc tgaacgagca gtttggtcgc accgttctgc
     1921 cgattggtga atcccactaa ttgtcttgag atccccccgc tcggggggat ttttttattc
     1981 gctaacgtcg cgtcttgcac tcttcggtct gaatgcagca accacttctg gtgccgtttc
     2041 aacgtatggc ccttccagca actgaatgca gtacggtacg ctggcaaaaa taccatgcac
     2101 cacaacgtta ccctcagcgt ccttcacacc ttccagcgtc tctttaatag acttcggctg
     2161 accgggtaag ttaaggatca gcgcctgttt gcgaatcacg cccacctgac gcgaaaggat
     2221 cgcagttggt acaaaatgca ggctgatctg gcgcatctgt tcaccaaagc caggcatctc
     2281 gcggtccgct actgccagcg tcgcatcggg cgttacgtca cgacgcgccg ggccagttcc
     2341 gcccgtggtg agcaccagat ggcaactcat ttcatccacc agctcacaca acgtttgctc
     2401 gatgatcgcc tgctcatcgg ggattaagcg ggtttccagt tcaaacggcg tggttagcgc
     2461 cgatgtcagc cattcttcca gcgctggaat gcctttatcc tgataaacgc cgctggatgc
     2521 gcgatcagag atggaaacta agccaatacg taaagtattc atcaagattt ttccgtttaa
     2581 acaatctcgt taaacagaat gatacactgc gaagggagtg acagacaggc ttcagaagag
     2641 gtagcgtgac cgacttcccg gtcacgctaa gaatgattac agcagatcgc cgatcatttt
     2701 ttccagtttt tcctggtcaa tagcaaactt acggatacct tccgccagtt tatctactgc
     2761 cattggatcc tggttgtgct gccacaggaa ctcggactca gtgatacgcg ccggacgcgc
     2821 tttcacttcg ccggtgtaag acagtttacg ttcgatagcc ccttcgctct ccgccagctc
     2881 tttcagcagt gccggtgcga tggtcagacg gtcacagcct gccagttcca gaatttcgcc
     2941 gatgttacgg aagcttgcgc ccataaccac ggtttcataa ccgtgctctt tgtagtactg
     3001 gtagatttca gatacagaaa ccacgcccgg atcttctgcc ggagcgtact ctttcttatc
     3061 ggtattcgct ttgtaccagt caagaatacg gccaacaaac ggcgagatca ggaacacgcc
     3121 cgcctccgca caagcacgag cctgagcgaa ggagaacagc agggtcaggt tacagttgat
     3181 accttctttt tccagctgtt ctgcagcacg gataccctgc caggtagaag ccagtttgat
     3241 cagaatacga tcgttgctaa tacctgcatc gttgtagagt ttgatcaggc gttttgcttt
     3301 cgcaattgac gcttcagtgt cataggaaag acgcgcatca acttcagttg agatacggcc
     3361 cggaaccagt ttcaggattt ccagaccaat atttactgcc agtttgtcgg tcgcgtccac
     3421 gatctgctgc gcgcgatcgt tgctctgctg tttcgcccag gcgacagcat catcaatcaa
     3481 cttacggtat tccggaatct gcgctgcgtt aagaatgaga gaagggttgg ttgtggcatc
     3541 ctgcggttga tacagcttca ttgccgcgat gtccccagtg tcggccacta cggtggtgta
     3601 ctgacgaagg gaggtcaatt tgtccgtcat gatagtattt ctctttaaac agcttgttag
     3661 ggggatgtaa ccggtctgcc ctgatgatat cacgacgcaa cgtcagcgca accgccgaca
     3721 agacggttat cgccaggcga gactgtttcg gatttctgac actgcgttga tatcgcccac
     3781 catttttata caaaacctca tgtatgctac gcagaagtta tcaagtacct cgtagcgtat
     3841 atacttctta aacaattggt aacgtttaca caggaaagtc atcgcgaccg gcaataagag
     3901 ggatatgcat gccagatttt ttctccttta ttaacagcgt cctttgggga tcggtaatga
     3961 tttacctgct cttcggcgca ggttgttggt tcacttttcg caccggattt gtgcagtttc
     4021 gctacatccg ccagtttggc aaaagtctta aaaatagcat tcatccacag ccaggcggtt
     4081 taacctcatt tcagtcattg tgtaccagtc ttgcggcgcg cgtgggtagc ggcaacctgg
     4141 ccggcgttgc gctggcaatt accgccggtg gaccaggtgc cgtcttttgg atgtgggttg
     4201 ctgcgtttat cggcatggcg acctcgtttg ccgaatgttc ccttgcacaa ctttataaag
     4261 aacgtgacgt caatgggcag tttcgtggcg gaccggcatg gtatatggcg cgcgggctgg
     4321 ggatgcgctg gatgggcgtt ctgttcgccg tctttttgct catcgcctac ggcataattt
     4381 tcagcggagt tcaggcgaac gccgttgccc gagccctgag tttttctttt gattttcctc
     4441 cgctggtgac gggcattatt ctcgctgtct ttgctctgct ggcaatcact cgtggtcttc
     4501 atggcgtcgc ccggctcatg caggggtttg tcccgttgat ggcgataatc tgggtactga
     4561 ccagcctggt gatttgcgta ataaatatcg ggcaacttcc ccacgtcatt tggtctattt
     4621 ttgagagtgc ttttggctgg caggaagcgg caggcggcgc ggcgggatat accttaagcc
     4681 aggcgattac taacggtttt cagcgcagta tgttttccaa tgaggtggga atgggatcga
     4741 cgccaaacgc ggcagcggca gctgcatcct ggcctccgca tccggcagca caagggattg
     4801 tccagatgat tggcattttt atcgacaccc tggtcatctg tacggcaagc gccatgctga
     4861 tattactggc gggtaacggc acaacctaca tgccgctgga aggtattcag cttatccaga
     4921 aggcgatgcg ggtgctaatg ggttcctagg gtgctgaatt tgttaccctc gtggttattc
     4981 tgtttgcctt cagctccatc gttgccaact acatttatgc cgaaaacaat ctcttctttt
     5041 tacgcctgaa caaccctaaa gcgatctggt gtttgcggat ctgcaccttc gcaacggtca
     5101 tcggcggcac cttgctaagc cttccgctga tgtggcaact ggcagatatc ataatggcct
     5161 gcatggctat taccaatttg accgccattt tactgctctc gcctgtggtt cataccattg
     5221 ccagtgatta tctacgccag cgtaaactcg gcgtgcgccc ggtgtttgat ccgttgcgtt
     5281 atccggatat cggtcgccag ctttctccgg acgcgtggga tgatgtttcg caggagtaat
     5341 cacaactatc gatcaactca ttctcttttt ttgctaaagt cggcataaat ttcctgcaag
     5401 gactggatat gctgattctt atttcacctg cgaaaacgct tgattaccaa agcccgttga
     5461 ccaccacgcg ctatacgctg ccggagctgt tagacaattc ccagcagttg atccatgaag
     5521 cgcggaaact gacgcctccg cagattagca cgctgatgcg catcagcgac aaactggcgg
     5581 gtatcaacgc cgctcgcttt catgactggc agccagattt cacgccggcg aatgcccgcc
     5641 aggcgattct ggcgtttaaa ggtgatgtct acaccggctt gcaggccgaa accttcagcg
     5701 aagacgattt cgattttgcc caacagcatt tgcgaatgct ttccggcttg tatggcgtac
     5761 tgcgcccgct cgatttaatg cagccttatc gtctggaaat ggggatccgt cttgagaatg
     5821 cccgagggaa agatctgtat caattctggg gagatatcat caccaacaag ctgaacgagg
     5881 cgctcgcagc acaaggcgat aatgtggtga ttaatctggc gtcagatgag tattttaaat
     5941 cggtgaagcc gaagaaattg aatgccgaga ttatcaagcc ggtgttcctc gatgagaaga
     6001 acggcaaatt taagatcatc agcttctacg ctaagaaagc acgcggtctg atgagtcgtt
     6061 tcattattga aaatcggctg accaaaccag agcaactgac tggttttaat agcgaaggtt
     6121 acttctttga tgaagattcc tccagcaatg gcgaactggt gtttaaacgc tacgagcagc
     6181 gttaagtttg tagtcaatgc cggatgcggc gtgaacgcct tatccggcct acaaaagcat
     6241 gcagattcaa tatattgcag aattaacgta ggcctgataa gacgcgccag cgtcgcatca
     6301 ggcgctgaat gccggattgt tacccggcat ttgtcattta gcggtgatgt ttgcctggac
     6361 catgaccgcc gtgatgatca tgaggagctt tcttatggtg gcgcggcggt ggcggcggtc
     6421 cgtgtgggtg ccagcgattg cctcgccatt cataatgttg tttccaccag ccgtggtcgc
     6481 gccagtgacc tccatcccag taatagccac gattatcacg atcgcctatc tgtaatttta
     6541 ctgacgggac taacgtaatt tccgcagcct gtgctgccat gggagcgacc agaaccaggg
     6601 aaagtgcgag tacgatagat tgcatctttt tcacaggtta ctccttttat cattgcccgt
     6661 tatgggtcga tatccggaga ttacgcgatg agaacgacgg ttgttattct ccgcaatcct
     6721 aatctctaag ttaccgcatt tattgataat ttctcctttt tccctgtcgt ttttctccat
     6781 aatttcttca taaaaaagcc gggctgcata aaagcaaacc cggcctgatt gagataatga
     6841 atagatttta ctgatgattc atcatcaatt tacgcaacgc agaaaaatcg gcgggcagat
     6901 tatgtgaaag caagggtaaa tcagcacgtt ctgccagctc ttttggcaga tccaacgttt
     6961 caccgagaat cgcttccacg ctctctttaa atttcgccgg atgcgcggtg ccgaggaaca
     7021 agccatattc gcctggattc aactggtcac gcagcgcacg atacgctacg gcagcgtgcg
     7081 gctccgaggt gtagcccagt tcttttaact cacgcattgt ctgttgcgtg gtttcatcat
     7141 ccacggctgc ataacccagc tctttcagtt gccagatttt gcggcggaac aactcttcca
     7201 cacgcggcca gttgttcggc tgactcacgt ccatcgcgtt ggataacgtc gcctgagtcg
     7261 ctttgggtga ccactgaccg tcgtgcagga aacgtggcac ggtatcgttc acgttggtcg
     7321 cagcaataaa acgtttcacc ggcagaccga atgacttcgc cagcagaccc gccgtcaaat
     7381 cgccgaagtt tccgcttggc accgagacaa ccagctggtt gcgcgcctcc tgcggcagct
     7441 gcgcaacagc ttcaaagtag tagcaaatct gcgccagcaa acggctgatg ttaatcgagt
     7501 tagccgagtt taaccccagc gccactttca gttcttcatc atcaaacgcc tgcttcacca
     7561 gcgcctgaca ggcatcgaaa tcgccgtcga tggcaacagt ttcgatattg ccgcccaacg
     7621 tacagaacag tttttcttgc agtggactga ttttgcctcg tggatagagg ataaccactt
     7681 tcacattcgg caaaccgtag aaagcatgag ccactgcagc tccggtatca ccggaggtcg
     7741 cggtcagaat ggtcactggc ttatcatccg caatatgggt cagcatttgt gccataaagc
     7801 gaccgccgaa atctttaaat gccagcgttg gcccgtggaa caattccaga caaccgacat
     7861 cgctttcaac attggcgacc ggagccggga aggcaaacgc cgcgcgcacg cgctcttcca
     7921 ggatttcctg tgggatttca tcaccaataa acgccgagag gatcttcgca ctgcgggtga
     7981 caaaatccag cttcagcatc tcatcaattt cagtcaggct gaattccggc aggtcgtgcg
     8041 gaaaaaacag cccctgattt ttgcccaacc cctgggttac ggcttgcgca aagctgacct
     8101 gctcgttgtg atctttcaga ttgtagagtt tcatttagtt ttccagtact cgtgcgcccg
     8161 ccgtatccag ccggcaaata tgaacaaaac cttcctgatt ttgcaggtag ttcttaccca
     8221 accagtcggc aacgcgctgg gcggtatccg gcttgtcaca tagagcgaac aaggtcgggc
     8281 cggagccgga gataccgctc gctaccgcgc cgatttccgc gaccgcctgc cgcgcctgcc
     8341 ggaagccagg cagtaaccgt tcacggtagg gttcagcgat aacatctttc atcagcttcg
     8401 cggcaagctc aggctgacgg gaatagcagg cgtgaatgaa gccagccaga tgtcgcccgt
     8461 gcgcaatgca atcctggcgg cgatactgcg ccggtaaaat agcccgggct tctgccgtcg
     8521 agactttaat tcccggatac gccagcaccc acagccactc atcaaaccct ggcacttgct
     8581 ggctgatgat gtcgttttct tcgatcatca actgcatacc accaagaaaa cacggtgcca
     8641 cgttgtcgta atgaatgctg ccggagatac gtccttccag ctcgcccatc aaagccagca
     8701 aacgagtgtc attaagcggc ttgccgcagt gttcattcat cgccatcagc gccgcgacca
     8761 ccgaacaggc gctggagcct aagcccgaac cgatcggcat attcttttcc agagtcatcg
     8821 ccactggaat ttgcttgcca agctcctggc aaaaacgctc ccagcactga taaacgatat
     8881 tttcccgtgg ttctgacggc agcttatcgg caaagcgtcc gagtttgttg agactgaatg
     8941 tctctgccgc ctcaaccgtg actacatctc cgagcaatgc accatcaacg ggtgccaccg
     9001 ccgccccgag cacatcaaac ccgacgctca tattggcact ggaagccggg gcataaactt
     9061 taaccatgtc agactcctaa cttccatgag agggtacgta gcagatcggc aaagacaccg
     9121 gcagcggtaa cgtcattgcc cgcaccatat ccgcgcagca ccaacggcag cggctgataa
     9181 tagtggctat aaaaggccag ggcgttttcg ccatttttca ctttgaacag cggatcattc
     9241 ccatccactt cggcaatctt cacgcggcag acaccatctt catcaatatt gccaacatag
     9301 cgcaaaactt ttccttcatc acgggccttc gccacgcgcg cggcaaagag atcgtcgagc
     9361 tgtgacagat tcgccataaa agcggcaaca tcaccctcgg cgttaaactc tgcgggcagc
     9421 acaggttcaa tttcaatatc cgccagctcc agttcacgtc ccgtttcacg agcgagaatc
     9481 aatagtttac gcgccacatc cataccagaa agatcatctc gcgggtccgg ttcggtataa
     9541 cccatttccc gcgccagcgt ggtcgcctcg gagaaactca tgccttcgtc taacttgccg
     9601 aagatataag aaagcgaacc tgaaagaatg ccggagaact tcaccaattc atcacctgca
     9661 ttgagcagat tttgcaggtt ctcaataacc ggtaatccag ccccaacgtt ggtgtcatag
     9721 aggaatttac gccgcgattt ttccgccgca taacgcaact gatgatagta atccatcgac
     9781 gaggtgttgg cctttttgtt cggcgtgaca acgtggaaac cttcgcgcag gaagtcggca
     9841 tattgatccg ccactgcctg gctggaggtg cagtcaacaa tgaccgggtt cagcagatga
     9901 tattctttca cgaggcgaat taagcgcccg agattaaacg gctctttggc ttgtgcaagt
     9961 tcttcctgcc agttttccag attaaggcca tgcacactag tgagcagagc cttcgagttg
    10021 gcaacgccgc agacacgtaa gtcgatatgt ttattcttca gccagctttg ctgacgcttc
    10081 agttgctcca gcagcgcacc gccaacgcca ccgacgccaa tcacaaacac ttcgataacc
    10141 tgatcggtat tgaacagcat ctgatgagta acgcgcacgc cagtggtcgc atcatcgtta
    10201 tttaccacga cagagattga gcgttcagaa gatccctgag caatggcgac aatgttgata
    10261 ttggcgcggg ccagcgcggc aaagaatttc gccgagatcc cacgcaaggt gcgcatacca
    10321 tcacctacca ccgagataat ggccagccgt tccgtcaccg ccagcggctc cagtaagcct
    10381 tctttcagtt ccaggtagaa ctcttcctgc attgcccgtt cagctcgcac acagtcgctt
    10441 tgtggaacgc agaaactgat actgtattcg gaagatgatt gcgtaatcag caccacggaa
    10501 atacgggcgc gtgacattgc agcaaagacg cgcgccgcca tgccaaccat ccctttcatc
    10561 cccgggccgg aaacgctgaa cattgccatg ttattcagat tggaaatgcc cttgaccggt
    10621 aattcgtctt catcacggct ggcaccaatg agcgtacctg gtgcttgagg atttccggta
    10681 tttttaatca ggcaagggat ctggaactgg gcgatggggg taatggtgcg ggggtgaaga
    10741 actttagcgc cgaagtagga aagctccatc gcttcctggt aggacatcga cttcaacaac
    10801 ctcgcatcgg gcacctgacg cgggtcgcag gtatagaccc cgtcaacgtc cgtccaaatc
    10861 tcgcaacaat cggcgcgtaa acaggcagcc agcaccgccg cggagtagtc ggaaccgtta
    10921 cgtccaagca ccaccagttc gcctttttca ttaccggcgg tgaaacctgc catcaacacc
    10981 atgtgatcag ccggaatgcg gcttgccgca atacggcggg tggactcagc aatatcgacg
    11041 gtagattcga ggtaatgccc cactgccagc agtttttcga ccggatcgat aacggtaacg
    11101 ttgtgaccac gcgcttctaa cacgccggcc ataatggcga tcgacatttt ctcgccacgg
    11161 caaatcagcg cagcgttgat gctatccggg cactgcccca acaaactaat gccatgcagg
    11221 acatgtttta tttgggcaaa ttcctgatcg acgaaagttt tcaattgcgc cagcgggaac
    11281 cccggctggg cggcggcgag tcccgtcaaa agttcggcaa aaatacgttc ggcatcgctg
    11341 atattgggta aagcatcctg gccgctaatg gttttttcaa tcatcgccac caggtggttg
    11401 gtgattttgg cgggggcaga gaggacggtg gccacctgcc cctgcctggc attgctttcc
    11461 agaatatcgg caacacgcag aaaacgttct gcatttgcca ctgatgtacc gccgaacttc
    11521 aacactcgca tggttgttac ctcgttacct ttggtcgaaa aaaaagcccg cactgtcagg
    11581 tgcgggcttt tttctgtgtt tcctgtacgc gtcagcccgc accgttacct gtggtaatgg
    11641 tgatggtggt ggtaatggtg gtgctaatgc gtttcatgga tgttgtgtac tctgtaattt
    11701 ttatctgttt gtgcgctatg cctatattgg ttaaagtatt tagtgaccta agtcaataaa
    11761 attttaattt actcacggca ggtaaccagt tcagaagctg ctatcagaca ctcttttttt
    11821 aatccacaca gagacatatt gcccgttgca gtcagaatga aaagctgaaa aatacttact
    11881 aaggcgtttt ttatttggtg atattttttt caatatcatg cagcaaacgg tgcaacattg
    11941 ccgtgtctcg ttgctctaaa agccccaggc gttgctgtaa ccagtcgacc agttttatgt
    12001 catctgccac tgccagagtc gtcagcaatg ccatgactcg ttcgcgtaaa gcttgcagtt
    12061 gatgttggtc tgccgttgtg tcacttttcg ccggttgttg tattaatgtt gctaattgat
    12121 agcaatagac catcaccgcc tgccccagat tgagcgaagg ataatccgcc accatcggca
    12181 caccagtaag cacgtcagcc aacgctaact cttcgttagt cagcccggaa tcttcgcgac
    12241 caaacaccag cgcggcatgg ctcatccatg aagatttttc ctctaacagc ggcaccagtt
    12301 ctactggcgt ggcgtagtaa tgatatttcg cccgactacg cgcagtggtg gcgacagtga
    12361 aatcgacatc gtgtaacgat tcagccagtg tcgggaaaac tttaatatta tcaataatat
    12421 caccagatcc atgtgcgacc cagcgggtgg ctggctccag gtgtgcctga ctatcgacaa
    12481 tccgcagttc gctaaaaccc atcgttttca ttgcccgcgc cgctgcccca atattttctg
    12541 ctctggcggg tgcgaccagg ataatcgtta tacgcatatt gccactcttc ttgaccaaat
    12601 aaccgcgaac agggtgatca ctgtcaactt attacgcggt gcgaatttac aaattcttaa
    12661 cgtaagccgc agaaaaagcc ctttacttag cttaaaaaag gctaaactat tttctgactg
    12721 cactaacggt ttaattgtta aaaaatgcta catatccttc tgtttactta ggataatttt
    12781 ataaaaaata aatctcgaca attggattca ccacgtttat tagttgtatg atgcaactag
    12841 ttggattatt aaaataatgt gacgaaagct agcatttaga tacgatgatt tcatcaaact
    12901 gttaacgtgc tacaattgaa cttgatatat gtcaacgaag cgtagtttta ttgggtgtcc
    12961 ggcccctctt agcctgttat gttgctgtta aaatggttag gatgacagcc gtttttgaca
    13021 ctgtcgggtc ctgagggaaa gtacccacga ccaagctaat gatgttgttg acgttgatgg
    13081 aaagtgcatc aagaacgcaa ttacgtactt tagtcatgtt acgccgatca tgttaatttg
    13141 cagcatgcat caggcaggtc agggactttt gtacttcctg tttcgattta gttggcaatt
    13201 taggtagcaa acatgcagac cccgcacatt cttatcgttg aagacgagtt ggtaacacgc
    13261 aacacgttga aaagtatttt cgaagcggaa ggctatgatg ttttcgaagc gacagatggc
    13321 gcggaaatgc atcagatcct ctctgaatat gacatcaacc tggtgatcat ggatatcaat
    13381 ctgccgggta agaacggtct tctgttagcg cgtgaactgc gcgagcaggc gaatgttgcg
    13441 ttgatgttcc tgactggccg tgacaacgaa gtcgataaaa ttctcggcct cgaaatcggt
    13501 gcagatgact acatcaccaa accgttcaac ccgcgtgaac tgacgattcg tgcacgcaac
    13561 ctgctgtccc gtaccatgaa tctgggtact gtcagcgaag aacgtcgtag cgttgaaagc
    13621 tacaagttca atggttggga actggacatc aacagccgtt cgttgatcgg ccctgatggc
    13681 gagcagtaca agctgccgcg cagcgagttc cgcgccatgc ttcacttctg tgaaaaccca
    13741 ggcaaaattc agtctcgtgc tgaactgctg aagaaaatga ccggccgtga gctgaaaccg
    13801 cacgaccgta ctgtagacgt gacgatccgc cgtattcgta aacatttcga atctacgccg
    13861 gatacgccgg aaatcatcgc caccattcac ggtgaaggtt atcgcttctg cggtgatctg
    13921 gaagattaat cggctttacc accgtcaaaa aaacggcgct ttttagcgcc gtttttattt
    13981 ttcaacctta tttccagata cgtaattcat cgtccgttgt aacttcttta ctggctttca
    14041 ttttcggcag tgaaaacgca taccagtcta tattacgagt cacaaacatc atgccggcca
    14101 gcgccaccac cagcacactg gttcccaaca acagcgcgct atcggcagag ttgagcagtc
    14161 cccacatcac tccatccagc aacaacagcg cgagggtaaa caacatgcta ttgcgccagc
    14221 ctttcaatac cgcctgcaaa taaataccgt tcattaacgc cccaatcaga ctggcgatta
    14281 tccatgccac ggtaaaaccg atatgttcag aaagcgccag caagagcaaa taaaacatca
    14341 ccaatgaaag ccccaccagc aaatattgca ttgggtgtaa acgttgcgcg gtgagcgttt
    14401 caaaaacaaa gaacgccata aaagtcagtg caatcagcag aatggcgtac ttagtcgccc
    14461 ggtccgttaa ttggtattga tcggctggcg tcgtcactgc gacgctaaac gcggggaagt
    14521 tttcccagcc ggtatcattg cctgaagcaa aacgctcacc gagattatta gcaaaccagc
    14581 tgctttgcca ttgcgcctga aaacctgact cgctagcttc ccgtttggct ggtagaaaat
    14641 cacctaaaaa actgggatgc ggccagttgc tggttaaggt catttcgcta ttacgcccgg
    14701 caggcaccac agaaagatcg ccggtaccgc ttaaattcag tgccatattc agctgcaggt
    14761 tctgcttccg ccagtcccct tcgggtaaag ggatatgcac gccctgcccg ccttgctcta
    14821 acccggtacc gggttcaatg gtcagtgccg ttccgttaac ttcaggcgct ttcaccacac
    14881 caataccacg cgcatccccg acgctaatca caataaaggg cttgcctaag atgatatttg
    14941 gcgcgttgag ctcgctaaga cgcgaaacat cgaaatcggc ttttagcgtt aaatcactgt
    15001 gccagacctg accggtataa atccctatct tgcgttcttc cacgttctga ttgccatcaa
    15061 ccatcaatga ctcaggtaac caaaaatgga taaaacttcg tttccgctcc acggttttat
    15121 cctcttcctg caccgtataa agctcggtca caggaatagc gataagcggc ccaacgagtt
    15181 tttgcggccc gctggtactt tggcgaatcg catcttccac atcgctacgg taatcggcac
    15241 gttcgacaat cacttgccga atcagcatta tcggaatcaa caacagcaat actgcaccaa
    15301 acaaggtggt cattttccag aacagggggg atttcaacat agtcttctcc tttgcaatgg
    15361 cagcaggata cgaaggctat gtgggaagaa tttgaagcta tgtgaagtga cggtgaagtc
    15421 gtagcgaggc cagcacgcca ccttcctgca cgttgcgcag cgtgacttcg ccgttaaaca
    15481 aacgggcgac ctcactgacg aacgccaacc ccagaccgct gcttttttgc ccatttgcac
    15541 gcggcaaaga gtaaaagcgt tcaaaaatac gtgacagcgc gtagtcagga ataccactac
    15601 cggtatccag caccttaagc gtgacgtgtt cctgatccac ttcggcgctt agcgttatgc
    15661 aaccgctctc gggagtaaaa tcgatggcgt tatccagtaa attccccagc gcctgatcca
    15721 gtaacgccgg ttcagcagca acattaacct cggtgggcat aacatgcaaa gtgatgtttt
    15781 tttctgccaa ctgcacggtg cgcgcttcgc tgacgcggcg aaataatgcc gccacatcaa
    15841 cagcagtcag aacgacttcc tgacgattct ccagtcttgc ctggcgtagt aacgtttcca
    15901 ccagcgcctg cattcgcgca ttttgtgtca gaatgttgtc agtaaaacga gccactactt
    15961 caggcggcgg accttcgcgt aaaatttccg ccgcgccacg aatcgccgcc agtgggcttt
    16021 ttagctcatg ggttaacgca taaacatact gctcaatata gtttttccct tccagcttca
    16081 cgcgcatact ttccagcgcc tgcgcgagtt tacgcagctc gctactaccg agatcgggaa
    16141 gaggaacggg cttattgtca gtgacggaat cagcatagcg agtgagccgg gcaatggagc
    16201 ggttgatcca ccaaaccatg cctgcgccaa tcaccagtgc aatccccaac aaaatggcgc
    16261 tggcccataa aattcgccgc tcgctgcgtt taatgaccgg agccatcgcc gcgttcggtt
    16321 tgcctacgct caaaacgcca ataagccgcg agccgcccat aatcggcgcg gcaacataca
    16381 tcaccgaact ttcgggatcg gcaggatttt gcaacgtgct gcgcgcacca tactgaccac
    16441 gcaacgttag ccagacgtca ttccagcgcg aataatcctg tccaacggct ttatttgccg
    16501 aatcgaacaa tactttgcca tgcgcatcgg tcatatagac atgatattca ttgcgcactt
    16561 tgttaatgcc accaatattg gcgcgaaacg ggcgatgttg tagctgatta aacgcctgcg
    16621 ccagttgccc atgcgttggg tccccagaga gcaaatcggg acgcgccagc tccgcaagta
    16681 acgttgccgt gtcgatcaac gtcccctccg ttgctcttcg cacgcccggt ttaacttctt
    16741 tgacaaaaat agccagtacg aaccaggccg ccaccgccac cagtaaaaaa tagcccagca
    16801 gcaaccgcat gccgatacgc attacaagcc cctcaggcta tatcccatgc cgcgatgagt
    16861 attaatcggt gaaagatcgg ggttgatggc gcgcagcttg gcacgcagcg ttttaatgtg
    16921 gatatcgacg gtgcgatcgt aggtgtcctg cgcatcttcc cacacgctat ccattagttg
    16981 ctggcgggac cagacgcggc ctggcgactt gagtaacgtc ttcagcaata aaaactcata
    17041 ccgcgtcagc gttaatggcg tgtcaaacca gctgatctgc gccgcgggtt cattcaattc
    17101 aaaatgacca atacggatga cgggagacgg cgtcgagaac ttcttcaccc gacgcagtaa
    17161 ggtgcgcacc ctggcgcaca cttcgcgggg tgaaaacggt ttagccacat agtcgtcagc
    17221 gccaatttcc agcccaagca ggcgatcgac ctcttcactt cgggccgtca ggaacagtac
    17281 aggtaacgcc ggatagagcg ccagtaactg gcggcacaat tcaaagccac tgatatcagg
    17341 caaaccaacg tcgagaatca tgacgtcggg aacctgctgg cgagctttat ccagcaccgg
    17401 caaacctcgc tcaaagacct cgacggcaaa accttcctgc tgcaacatgt agaccagcgt
    17461 gtcggctatc ccttgctcat cttccactaa ccagaccgtt tcccgttgca taaatcgcct
    17521 ctgttattgc cgccacggca tgacaggaac cgcgctaatc gcgtttttcg gcgaaccttc
    17581 tacaactttg tcggagtaag ccagataagc gagcgcgttg cgtttggcgt cataaaagcg
    17641 cacgacctgt aacgacttaa agaccaggga cgtgcgtttt ttgaatacta cctcgccctg
    17701 agctttgccg tttttaatac gatccgacag ttcaatcggc ccgacttgct gacaagaaat
    17761 ggccgcatcg gaggtatctt ctgccaggcc caatccccct ttaataccac cggttttcgc
    17821 ccggctcaca taacaggtga catttttcac atcgggatcg tcaaaggctt ccacaacaat
    17881 tttgtgatcc gggccgatca ttttaaatac ggtgtcgacc gaaccaatct cttctgcatg
    17941 agccaatggc cccagcatta tcaggcttaa agacaggatc aaatgcttgt atttcatatt
    18001 gttaccattt ttatctatta cacgagcaat tagttcgtca cggtaaataa catattgatt
    18061 aagaacaaaa tctcaattct tttatttccg tcaagcccta aaacatactc tactaagaaa
    18121 aaaacactga atgctaaaac agcaaaaaat gctattatcc aattacctga tgtcaggtgc
    18181 tcgttgttga aaggatgagg atattttatg gatcaggccg gcattattcg cgacctttta
    18241 atctggctgg aaggtcatct ggatcagccc ctgtcgctcg acaatgtagc ggcgaaagca
    18301 ggttattcca agtggcactt acagagaatg tttaaagatg tcactggcca tgctattggc
    18361 gcgtatattc gtgctcgtcg tttgtcgaaa tcggcggtcg cactacgcct gactgcgcgt
    18421 ccgattctgg acatcgcgct gcaataccgc ttcgactctc aacagacatt tacccgcgca
    18481 ttcaagaagc agtttgccca gactcctgca ctttaccgcc gttctcctga atggagcgcc
    18541 tttggtattc gcccgccgct acgtctgggt gaattcacaa tgccagagca caaatttgtc
    18601 accctggaag atacgccgct gattggtgtt acccagagct actcctgttc gctggagcaa
    18661 atctctgatt tccgccatga aatgcgttat cagttctggc acgattttct cggcaacgcg
    18721 ccgaccattc cgccggtgct ctacggcctg aatgaaacgc gtccgagtca ggataaagac
    18781 gacgaacaag aggtattcta taccaccgcg ttagcccagg atcaggcaga tggctatgta
    18841 ctgacggggc atccggtgat gctgcagggc ggcgaatatg tgatgtttac ctatgaaggt
    18901 ctgggaaccg gcgtgcagga gtttatcctg acggtatacg gaacgtgcat gccaatgctc
    18961 aatctgacgc gccgtaaagg tcaggatatt gagcgatact acccggcaga agacgccaaa
    19021 gcgggagatc gcccaattaa tctgcgctgt gaactgctga ttcctatccg ccgttaacgc
    19081 tgcagctcat ctaatgcagg ggcgtccaga tgcgagatgt cccctgcggt ttcaacgacc
    19141 cagccagacg ccagccacag gctttcctga taatccacgc gcgaaataga acagttacgc
    19201 agacgtaagc ggcgctctgc ccatgctggt aatccgagaa tcgtactcac cagacatccc
    19261 agtgcaatac catggctcac cagcagcggt cggcttccct gcggtaagtc ccggcaagat
    19321 tccagtgccg cattgacgcg atcgctgagc tcctgcattg actcgccttc aggaatacgc
    19381 ccgtcaacgg tgccattgac cagctgccga cgccagttct cttcttcttc ggtcagagaa
    19441 tcgatatgtc ttttttccag cacacccatg tttaattcac gcaggcgaga atcaaagatg
    19501 atgtcacagc cgcaggcctg ggcgatgatt tccgccgtac gccgggtgcg tcctaaatcg
    19561 ctactgataa tatgcgtaat gccaagctct ttggcacggg ttgccacctg catcgcctgt
    19621 tgctcacctt tggcggtcag cgggctgtca gactggccct gaatacgtcg ctcggcgttc
    19681 cactgcgttt caccgtggcg gactaggtat acctgtaaca tgcttttttt ccgtatactg
    19741 ctgtaacgtt aattttctga gtaatgctga ttatgcacca agttgtctgt gcgaccacca
    19801 atcccgctaa aattcaggcc attctgcagg catttcacga gatcttcggc gaaggatcct
    19861 gccatattgc atccgttgcc gtcgagagcg gtgtaccgga acaacccttt ggcagtgagg
    19921 aaacgcgcgc tggcgcacga aatcgggtag caaatgcccg ccgtttactt ccagaggctg
    19981 atttttgggt ggcgattgaa gctggcattg atggcgacag cactttcagt tgggtggtga
    20041 ttgaaaacgc cagccagcgc ggcgaggcgc gttcggcaac tttgccgtta cctgcggtga
    20101 ttctggaaaa agttcgtgaa ggcgaggcgc ttggtcccgt gagtcgcgtt ataccggtat
    20161 tgatgagatt ggtcgtaagg aaggggcgat cggcgtgttt accgccggga aactcactcg
    20221 cgccagcgtc tatcaccagg cggtgatcct ggcgctgagt ccgtttcaca atgccgtgta
    20281 tcaggcattg taggacggat aaggcgttca cgccgcatcc gacattcggt gcacgatgcc
    20341 tgatgcgacg ctgccgcgtc ttatcaggcc tacaaaatca atcgcttttc agcaacacct
    20401 cttccagcca ctgacgcagc tccacgggcg cggctttcag gctgttagat ccacgcgtaa
    20461 tcgtcgcgat gcccgcgccg agttcatttt ttaactcacg ctgactcatt tcgccacgca
    20521 acagctcttc gacaatacgc acgcgagtcc ccaacgcttc gcgctcatct ggcgtcagca
    20581 tcaggtttaa caacggtaaa tggagatcgt tttggtaggc attcttaagc aggtcgacaa
    20641 aacgtaacca ctcctggtga cgctgttctg ccatcgctgc tgaatagggt gattgttggg
    20701 ccataatatg tcgccattgt tagcggggga agcaaaatga ctcccccggt tgtactcgct
    20761 aaagagtacg atagcatatc ataaacgtgc ggatcagtaa cgacgtcccc attccgtggc
    20821 gctcatcaac gtcggtttat cccccatgaa atagcggtag taagcatcat aagccagcac
    20881 gttcttcaca taaccgcgcg tctcggagaa cggaatactc tcgacaaatg ccacagcgtc
    20941 gatacgcccg gcgctgttgc caagccaggt tcgcacccgt ccaggtccgg cgttataagc
    21001 tgctgaggag aaaatacggt tattgccaaa ctgctgataa acatattgca ggtaactggt
    21061 gccaatgttg atgttcgttt ccggatccag caattgccca gggctgctat aaccaggaat
    21121 agagaacatc ttcaccgtat gggtcgctgt accaggcata atctgcatca ggccgctggc
    21181 ccctaccggt gatttcactt tcggattcca ggcgctctcc tgacgggcaa tcgccatcgc
    21241 atagctttgc gggatctctt tcccactggt gtaacgtttg aaaagatcgt tgtaagccag
    21301 cgggaatcgc tcttccagat gatcccaaag cttcccggcg atcgttgcct gaacgctaag
    21361 atcccaccat tggttattga aagcataccg agccagttga gcctgctctg tttttgactt
    21421 gctcttcacc agattggccc actcgctacg cgcggtatta tcgagattcc agtacatcaa
    21481 ctcgcgcacg cgcgccatct ccggcccctg agtcagggtg ctgtcaacat tctgcggcgc
    21541 tttatcaatc ttcagctcat actcttcacc gatgcgttgt gctgcaacca tcgggtagaa
    21601 accacgctgt tgtatgagtt gatgcaaaat ctctttcgct tcagcttcac gtccgcgttc
    21661 cagcaataaa tccgcctgcc agtaacgcca ttcatctttc tcttttgctt ccatcggcag
    21721 acgcgccagc caggtattca ggccgcgacg atcgccggtg ccaagcgcca ttcgcacacg
    21781 gcgttcaatc aacgacgtgg attgcgagcg cataatggcg tcatcgcgcc atttcgcctg
    21841 ctcatcggtg acatcattgc ccatcaaacg ccaggcgacg atatcgcgca gctcctgaat
    21901 ctgatcttca ttaagctgct gcgcctgggc aagcgacggg atcatcagcc gtgcattttc
    21961 ggcatcctgc cgcgccacac tggcaaacgc caccgccgcc atttgacggg taaaatcggt
    22021 cgcgccggtt gtacgcgcga aggtcagtac cgtattaggg ttgttcgcca gtgaaatgat
    22081 tgccgaggcg atagtctggt aatcggcagg catctgccct gccagcactg ttaccaggcc
    22141 tgtgttaccc gctttcatcg ccagacggat acgctctaaa tacgccagcg gatcttgttt
    22201 acctgacgca cgccagacgc taaataactt gtcacaggcg ttaggctggc tcttgccggt
    22261 tagccacagc tctttcgccc cttgccaggc ttcttcactc tgcccggtgt tccatttcgc
    22321 atagtagtaa ttacattgcg cttcggtagt tccgggcttt tccgggctaa aggctaacaa
    22381 gccacgccag tcttcacgcc gcgccagttc attgacgaaa cgagattgca gcgtgcgagc
    22441 gggaggaagc gtggggttag cgcgaacaaa gttagtgacc gtcaccgccg gttgattcat
    22501 caggtcatcg gtgatctggc ggtattccag gtagggataa agcggataat ccttcagtcc
    22561 aggcatcatt tgttcgacca catccatctg tcgattatcc caggcctgct tgatttgcgc
    22621 gtaacggcta cgctgctcat ccagtgagtc ggctcgcgcc acgctgctga ccgtcagcag
    22681 acagacaccg gcagccaaca gccgccaggt aacttgtttg gctttttcca caagcgcatc
    22741 ctctaagtgt aaatacatca atgcagcgtc atgccgcgta atagatttat gctaaccagt
    22801 cattgccgtt tacgccacgt tacggacact tttttacttt tactgcgaag gtgatcgacc
    22861 aactgggatt aggcggggaa acaggctaca cttcgccttt gaaaactcat catcacgata
    22921 aacatagagg cgaagtccaa cgtggctcaa ttcgtttata ccatgcatcg tgtcggcaaa
    22981 gttgttccgc cgaaacgtca tattttgaaa aacatctctc tgagtttctt ccctggggca
    23041 aaaattggtg tcctgggtct gaacggcgcg ggtaagtcca ccctgctgcg cattatggcg
    23101 ggcattgata aagacatcga aggtgaagcg cgtccgcagc cagacatcaa gattggttat
    23161 ctgccgcagg aaccgcagct gaacccggaa cacaccgtgc gtgagtccat tgaagaagcg
    23221 gtttcagaag tggttaacgc cctgaaacgc ctggatgaag tgtatgcgct gtacgccgat
    23281 ccggatgccg attttgacaa gctggccgct gaacaaggcc gtctggaaga gatcattcag
    23341 gctcacgacg gtcataacct gaacgtacag ctggagcgtg cggcggatgc gctgcgtctg
    23401 ccagattggg acgcgaaaat cgctaacctc tccggtggtg agcgtcgtcg cgtggcgttg
    23461 tgccgcctgc tgctggaaaa accagacatg ctgctgctcg acgaaccgac caaccacctg
    23521 gatgccgaat ccgtggcgtg gctggaacgc ttcctgcacg acttcgaagg caccgtggtg
    23581 gcgattactc acgaccgtta cttcctcgat aacgttgcag gctggatcct cgaactggac
    23641 cgcggtgaag gaattccgtg ggaaggtaac tactcctcct ggctggagca gaaagatcag
    23701 cgtctggcgc aggaagcttc acaagaagcg gcgcgtcgta agtcgatcga gaaagagctg
    23761 gagtgggtac gtcagggaac taaaggccgc cagtcgaaag gtaaagcacg tctggcacgc
    23821 tttgaagagc tgaacagcac cgaataccag aaacgtaacg aaaccaacga actgtttatt
    23881 ccacctggac cgcgtctggg cgataaagtg ctggaagtca gcaacctgcg taaatcctac
    23941 ggcgatcgcc tgctgattga tgacctgagc ttctcgatcc cgaaaggggc gatcgtcggg
    24001 atcatcggtc cgaacggcgc gggtaaatcg accctgttcc gtatgatctc cggtcaggaa
    24061 cagccggaca gcggcaccat cactttgggt gaaacggtga aactggcatc ggttgatcag
    24121 ttccgtgact caatggataa cagcaaaacc gtttgggaag aagtttccgg cgggctggat
    24181 attatgaaga tcggcaacac cgagatgcca agccgcgcct acgttggccg ctttaacttt
    24241 aaaggggttg atcagggtaa acgtgttggc gaactttccg gtggtgagcg cggtcgtctg
    24301 catctggcga agctgctgca ggttggcggc aacatgctgc tgctcgacga accaaccaac
    24361 gacctggata tcgaaaccct gcgcgcgctg gaaaacgccc tgctggagtt cccgggctgt
    24421 gcgatggtta tctcgcacga ccgttggttc ctcgaccgta tcgccacgca catcctggac
    24481 taccaggatg aaggtaaagt tgagttcttc gaaggtaact ttactgagta cgaagagtac
    24541 aaaaaacgca cgctgggcgc agacgcgctg gagccgaagc gtatcaagta caagcgtatt
    24601 gcgaagtaat gcgtaaaatg ccggatgcgg cgcgaacgcc ttatccggcc tacaaaacca
    24661 gcaatttcaa tacgttgtaa aactgtaggc ctgataagac gcggtaagcg tcgcatcagg
    24721 cattgagcac cgaatgccgg atgcggcgtg aacacctttt ccggtctaca acagccttag
    24781 catcctcaat ttcgtggcgg gtcacccgtg caggtttttt caaaccttgt ttaatctcga
    24841 cagcctcttc cagagaggtt ttcaactcat caaaaaaact cattttatac ctcgcctttg
    24901 agcagtttcg tcacggttat ccttgctccc ccatcatctc ccgcaccagc tccacgcagc
    24961 gcaggaaacg gctgtcgtaa tcgtcctctt caacccgcac gaattcgata ttgttctctt
    25021 ccagcatctc caccagcaag ttctggaact ctttgcgatc caccgaactg ccgaggctgc
    25081 gtaaaccatc cgccacccac ggcgtgttgt tctccagcag gatcaccaga tcgaaacggt
    25141 attcatcaat cagcgcctgc acgaacggat gctcacgccc ttcgtacttt ttgcagaacg
    25201 cctgggtagt gacaaaatcg gtgtcaataa acgccacttt attggcgtat ttcactgcaa
    25261 aatcaatgta ttgtgcgtga cccaacgcga ttttatcgta gtcggaatac tgcaatgcga
    25321 tttcatcacc gccgaggtgt gaaaagacat aatcgcggcc atattcccac gcactggtgg
    25381 tgttgaagat attggcaagt ttgtttacca gggtggattt accgctcgac tcgccgccaa
    25441 ggatcgccac ggtacgcaca aaaaacggct tcacttcggt aggaatatat tcccagtagc
    25501 ggaacgggtt ttcgcggatc tgcgcaccgc tgatactcat aaaggtacgt ttcggatcga
    25561 ccagcaccgt ctcgatcccc agatgttcca tatactgtgg cgcatcggct tcttccgagg
    25621 tgtagatcag gtccggctgg atcccctttt cagccataaa ctttttgatg ccgttgctcc
    25681 acacatccca gccatgcgga tacggctcca tgccctcttc gttgaaagca tgaatacgga
    25741 tatttttctg atatttaaag gtttgcaata gccagcgcag acgatccggc accgtgggct
    25801 gctgcgacat ggcgctgtct tcaaacaacg cgcggtcgcg ggtatcgtca aaacccataa
    25861 tgatatgcag ctcgtcaacc tggctacagg cgcgctggat aaggtagata tgtccggtat
    25921 gcagcgggta gaacttaccg aatacgacac caatggtttt cttctgccgg ggaaattcaa
    25981 gccccaaaaa acggtgcaat gcctccagct tttgcgcgct ggggctttta attttggcat
    26041 tcagtaactg gcttaaatac cctttggtca taccgctggc atcagctacc tgctgtagcg
    26101 tgcagccctg ttgcttgatg gcagttttca ggtaatcaaa tgacgacata tctccctccg
    26161 tatctctcat tataagtcgt cgaacacgct aagcgcgtcg gagagttttt taacgccaaa
    26221 aatctgcatc ccttccggcg cttttttcgg tacgttagcc gccggaacaa tcgcccggcg
    26281 aaaaccgtgt ttcgccgctt cagaaatccg ctcctggcca ctgggcaccg ggcggatctc
    26341 ccctgccagc ccgacttcac caaacaccac cagatcctgt ggcagcggtc tgtcacgcag
    26401 gctggaaacc atcgccagca gtaacgctaa gtcggcgctg gtttcggtta ccttcacgcc
    26461 gccgaccacg ttcacaaaca catcctgatc ggccatttgc agaccaccgt gacggtgcaa
    26521 gacagccagc aggattgcca gacggttttg ttccagcccc actgccacgc ggcgcggatt
    26581 cgccattatc gagtgatcga ccagcgcctg aatctccacc agcaacggac gcgttccttc
    26641 ccacaccacc atcacggagc tgccggaggt cacttcatct ccgcgactta agaaaattgc
    26701 cgaagggttg ctgacttcac gcagcccctg ttcggtcatc gcgaagacgc ccagctcatt
    26761 caccgcgccg aagcggtttt tatggctgcg taaggtgcga aaacgggagt cggcatcgcc
    26821 atccagaagc accgaacagt cgatacagtg ttccagcact ttcgggccag ccagcgaacc
    26881 atctttggtt acgtgtccaa ccatgacaat cgccacaccg cgcgttttgg cgaaacgcgt
    26941 cagataagcc gccgtttcac gcacctgcgc cacgctacca ggcgatgatt gtacatccgc
    27001 catatgcatc acctggatcg agtcaattac catcagcttc ggttgctctt cttcggcaat
    27061 caggcagatc tgctcgatgc tggtttccga caacatattg agattgtcag tcggcaggcc
    27121 aaggcgatga gcgcgcattg ccacctgttg cagcgactct tcgccggtga catacagcgt
    27181 tttcatctgc tgggccagtt tgcacagcgt ttgcagcaac agcgtggatt tccccgcacc
    27241 agggttaccg ccaatcagaa tggcacttcc tggcaccacg ccgcctccta gtacgcggtc
    27301 gaactcttta aatccggtgg aaaaacgcgg cagctcttca aggctgatat cggagagttt
    27361 ctggactttt gccaccccgg cgctaccggc atagccgctg agacgctcgt tacgcgccac
    27421 cgttggcgac gcagcaagac gcacctcggt gatggtgttc caggcatgac aggcactgca
    27481 ctgcccctgc cagcgcggat aatcggcccc gcattcatta caaacaaagg cgcgttttgg
    27541 agcttttgcc acggtgtacc tcgttaatgc tgtgccgccc gcaggatgac gggcaggcaa
    27601 ttacttctga ttcaggctgc cagagaggat gcagaatacc cccatcaggt cagcgtgacg
    27661 gatggtgact tccgcctttt cattcacttt tggcttggca tggtaggcaa tccccagccc
    27721 tgctgctttg atcatcggca ggtcattagc tccatcgcca atcgccacgg tctgcgccag
    27781 cgggatttca tactcctgcg cgaggcgagt cagagttttc gctttgtact gcgcgtctac
    27841 gatgtcgccg atcacattgc cggtaaattt accgtccatg atctccagtt cattggctac
    27901 cacggctgtc aggcgcagct tgtcgcgcag gtattcagca aagaaagtaa agccgccgga
    27961 ggcaatcgcc actttccagc ccagcgtttc cagcttgagc accagctgcg ttaagcctgg
    28021 catcagcggc agattttcac gcacctgttg cagaatattg gcgtcagcgc ctttcagcgt
    28081 cgccacgcgg ctgcgcaggc tggcggtaaa atcgagttcg ccgcgcatcg cccgttcggt
    28141 tacctccgcc accatctcgc ccgttccggc cagcttggca atttcatcaa tacattcaat
    28201 ctggatggcg gtggaatcca tgtccatcac cagcaaaccc ggcgtgcgca ggtgcgggat
    28261 tttccccagc ggggcgacat ccagctgcgc ttcgtgggcc aggcgtgtag cccgtgcggt
    28321 gagtgaacct gccagacgaa tcacctgata atcttccacg caccaggcgg caacaatcac
    28381 catcgccgca cccagtttgc tctggtattg ggtcagacgc tgtttatcca gcccacgacc
    28441 atacagcagc cagccgctac gacctgcgtg gtaatccagt ggcatcactt catcaccact
    28501 taatgaaaga ggcagacccg gccataaaga gacatcttca ggcaggtcgc accaggtaat
    28561 gttaggcatt aaggctcctg taaaatcgtt cgaagcaggg aaaataacgc atgaggctac
    28621 cttgtatcca ttgcttctgg caacattaag tctcaaattt tcaaagggtg gaagatggct
    28681 cgcacaaaac tgaaattccg gctacatcgg gcagtgattg tcctgttctg tcttgccttg
    28741 ttagtggcac tgatgcaggg ggcgtcatgg tttagtcaaa accaccagcg acagcgtaat
    28801 ccacagctgg aagaactggc ccgcaccctg gcgcgtcagg tgacgctgaa cgttgcaccg
    28861 ctgatgcgta ccgactcacc ggatgaaaaa cgcattcagg cgatcctcga tcagttaaca
    28921 gatgaaagcc gtatcctcga cgcgggtgtg tatgacgaac aaggcgatct tatcgcacgt
    28981 tctggcgaaa gcgtcgaagt gcgcgatcgg ctggcgctcg acggtaaaaa agcaggcggc
    29041 tattttaacc agcagattgt cgagccaatt gcgggtaaaa acggaccgct cggctatctg
    29101 cgcctgacac tcgacaccca tacgctcgcc accgaagccc aacaggtgga taacaccact
    29161 aacattttac gcctgatgtt gctgctctca ctggcaatcg gcgtagtgct gacccgcacg
    29221 ctgctacagg gtaaacgcac ccgctggcag caatcgccct tcctgttaac cgccagcaaa
    29281 ccggtgccgg aagaggaaga aagcgagaaa aaagagtgac ccattactac aagaaaggaa
    29341 atcgttatgt ccacattacg cctgctcatc tctgactctt acgacccgtg gtttaacctg
    29401 gcggtggaag agtgtatttt tcgccaaatg cccgccacgc agcgcgttct gtttctctgg
    29461 cgcaatgccg acacggtagt aattggtcgc gcgcagaacc cgtggaaaga gtgtaatacc
    29521 cggcggatgg aagaagataa cgtccgcctg gcgcggcgca gtggcggtgg cggcgcggtg
    29581 ttccacgatc tcggcaatac ctgctttacc tttatggctg gcaagccgga gtacgataaa
    29641 actatctcca cgtcgattgt gctcaatgcg ctgaacgcgc tcggcgtcag cgccgaagcg
    29701 tccggacgta acgatctggt ggtgaaaacc gtcgaaggcg accgcaaagt ctcaggctcg
    29761 gcctatcgcg aaaccaaaga tcgcggcttc caccacggca ccttgctact caatgccgac
    29821 ctcagccgcc tggcaaacta tctcaatccg gataaaaaga aactggcggc gaaaggcatt
    29881 acttcagtgc gttcccgcgt gaccaacctc accgagctgc tgccggggct caccatgagc
    29941 aggtttgcga ggccataacc gaggcctttt tcgcccatta tggcgagcac gtggaagcgg
    30001 aaatcatctc cccggacaaa acgccagact tgccaaactt cgccgaaacc tttgcccgtc
    30061 agagtagctg agagtggaac ttcggtcagg ctccggcatt ctcgcatctg ctggatgaac
    30121 gctttagctg gggcggcgtg gaactgcatt tcgacgttga aaaaggccat atcacccgcg
    30181 cccaggtatt taccgacagc ctcaaccccg cgccgctgga agccctcgcc gggcgactgc
    30241 aaggctgcct gtaccgcgcg gatatgctgc aacaagagtg cgaagcgctg ttggttgact
    30301 tcccggacca ggaaaaagag ctacgggagt tgtcgacgtg gatagcgggg gcggtaaggt
    30361 aactcgctga ttaaaatatc caaggaagga aatatgatcg atctggaaaa ccaggaacgg
    30421 gagattatca atttaatgtt cagccagcgt atttcctggc tagccgccgt gcgtatcagg
    30481 cataaattat cgcttgctga agtaagtaaa atgttaggta tctcgattaa ttctctaaaa
    30541 cagatcgaaa agacggaacg gcttagctca aacatcaaaa gcaaaatggc agaaatttat
    30601 ggttgtccac cagagttgct gatatgccct tcctggatga cggctgaaca taaatgaata
    30661 gattcgtgaa tagatacttg aatgtttttt attttctatc catatgaaaa ataatagtaa
    30721 atctataaat cagttgctat tgatgaatag atactaaaaa gccggagcag tcccccactc
    30781 cggcttaaca cgacaatcgc ctttcagcga aacacaatta ctctttatcg cccagcagaa
    30841 cggattccag tgcgattttg atcatgtcgt tgaaggtggt ctgacgctca gcggcagtgg
    30901 tctgctcgtg agtgcggatg tggtcagaca cggtgcagat ggtcagggct ttcgcgccaa
    30961 actctgcagc cacgccgtag ataccagccg cttccatttc cacgccgagg atgccgtatt
    31021 tttccatcac gtcgaacatt tcgccgtccg gagagtagaa caggtcagcg gagaacaggt
    31081 tacccacgcg agcatcaacg cccagtgctt tagctgcatc tactgcgtta cgcaccatgt
    31141 caaagtcagc gatagcggca aagtcatggt ctttaaaacg gatgcggtta actttggaat
    31201 cggtgcaggc ccccataccg ataacgacgt cgcgcagttt tacgtgcggc agaactgcgc
    31261 cacaggaacc aacgcggata attttcttca cgccgaaatc ggtgatcagt tctttggtgt
    31321 agatggagca ggacgggata cccataccgt gacccattac ggaaattttg cggcctttgt
    31381 aagtaccagt gaagcccagc ataccgcgaa cgttgttcac ttcacgggca tcttcaagga
    31441 aagtttcagc aatatacttc gcacgcagcg ggtcgcctgg catcaaaact acgtcagcga
    31501 aatcgcccat ttctgcatta atgtgtgggg tagccattgt tttatccttt ttgtgacata
    31561 acaaaggcgg agtcaacgct ccgcccaaat ccatcagaac atggctttgc catattccat
    31621 atcagaagta ccaaaatatt ttgccagagt ctggccgata tccgcgaagg tttcacggtg
    31681 acccagtgag cccggtttta ctttcgggcc atataccagt accggaatgt gttcacgcgt
    31741 gtggtcagta ccggtccagg tcggatcgca accgtggtca gcggtgagga tcaggatgtc
    31801 gtcatcgcgc agcagagaca tcagctccgg cagacggcgg tcgaacagtt ccagacccgc
    31861 agcataaccg gcgacgtcgc ggcggtggcc ccaggaagag tcgaagtcaa cgaagttggt
    31921 gaagacgatg gtgttatcac ccgcttcttt catctctttg atggtggcgt caaacagcgc
    31981 gtccaagcca gtcgctttca cttttttggt gataccgcag ttggcgtaga tgtctgcaat
    32041 tttaccgaca gaaaccacct ggccgtgttt ttcatcaacc agtttctgca gcacggtcgg
    32101 tgctggcggc tcaacagcca agtcgtgacg gttaccggta cgctggaagt taccagcttt
    32161 gtcaccgata aacggacgag cgataacacg accgatattg tagccgccgt tggtcagctc
    32221 ttcacgggcg atttcgcaca gttcgtagag tttatccaga ccgaaagttt cttcatggca
    32281 ggcaatctgg aacacggagt cagcggaggt atagaaaatc ggcttgccgg ttttcatgtg
    32341 ctcttcgccc agttgatcca gaatgaccgt accggaagag tggcagttac cgaggtaacc
    32401 cggcagatta gcgcgttcga ccagtttatc cagcagctct tgcgggaagc tgttttcgtg
    32461 atcggagaaa tatccccact caaacagaac cgggacaccg gcgatttccc agtgaccaga
    32521 cggggtatct ttaccggatg acatttcgtg cgcccatgcg tacgcgccga taacttcagc
    32581 gttgccgtcc attcccgccg gaatgaaacc ggtagaacct tcgtgtgctt tcgccagccc
    32641 cagacgggtc agatttggca gattgagcgg ccctttacga ccattatcag cttcgccttt
    32701 ggcacaagct tctgcgatat gacccagggt gtcagccccg acgtcaccaa agcgttctgc
    32761 atcttctgta gcgccgatgc cgaatgagtc cagcaccata ataaatgcac gtttcatatg
    32821 ttctccgtac tcagtgcttc aaatttaaat gatcagatca gtataccgtt attcgctgat
    32881 acggcgatag acagttggtg tgctttccgg tgcgttatcg gcaagtttaa ttgccgcttt
    32941 caccgctttc gccgcttcct gccagctgtt ttcgtctttc gcgaggataa ccgccagcgg
    33001 acgctgaccg tctacctggt cgcccagacg cgccatatca gtaaagccga cgctgtaatc
    33061 gatggtgtca gatgcctgac gacgaccgcc gcccattgca actactgcca tccccagcgc
    33121 gcgggtatcc atttcactga caaaaccttc ggtatcagca tagactgctt tcgtcagcat
    33181 cgctgtcggc agatacttcg cgtagttctc aacgaagtcg gtcgggcctt tttgtgccgc
    33241 taccatacga ccaaagactt ctgccgcttt accgttgtcc agcaccgcct gcaatttcgc
    33301 gcgcgcttcg gcgtcatctt tcgccagttt gccggagatc aacatctcca cgcacagcgc
    33361 catcgtgaca tcaaacagac gcgggttacg atactcaccc gtcaggaact gcaccgcttc
    33421 acgaacttca accgcgttac ctgcactgga ggccagtacc tgattcatgt cggtgagcag
    33481 cgcggtggtg cgcacgccag cgccgttagc cacgccaaca atcgcttcgg caagggcttc
    33541 agagagttcg taggtcggca taaacgcgcc gctacccact ttcacgtcca tcaccagcgc
    33601 gtccagacct tccgccagtt tcttcgccag gatcgaggca gtgatcagcg ggatggagtc
    33661 cacggttgcg gtaatatcac gggtcgcgta gaaacgttta tcagccggag ccagtgaact
    33721 ggtctgaccg ataatcgcca cgccgacgtc tttaataatt tcgcggaaac ggttgtcatc
    33781 cgggaaaatg tcgaagccag ggatggattc cagtttgtcg agcgtaccgc cagtatgacc
    33841 gaggccgcga ccggagatca tcggaatata gccgccgcag gctgcgacca tcggccccaa
    33901 catcagcgaa gtgacatcgc cgacgccgcc ggtcgagtgt ttatcaacaa tcgggccatt
    33961 cagatgcagg cttttccagt cgagaacggt tcctgaatct cgcatcgcca tggtcagcga
    34021 gacacgctca ggcattgtca tatcgtggaa gaaaatggtc atcgcgaggg cggcaatctg
    34081 cccttcggaa atagtgttgt cgcgaatacc gttgataaag aaacgaattt cttcatcgct
    34141 cagcgcatga ccatcacgtt ttttacgaat aatttcttgt gcgagaaaca aggtaacctc
    34201 ccgaggaaaa gagtatgagg cggcttttgc cggatgcgtc tacgccttat ccggcctacg
    34261 ggtagatacg tctcgtaggc ctgataagcg cagcgcatca ggcgtaaagc atcttactta
    34321 gtagctgctg gcgctcttac cgtcgccgtg gcccagcgct ttcagcaggc ttgccagcag
    34381 gctggaagca ccaaagcggt agtgacgcgc atcagcccag tcagcaccga acagttcatc
    34441 tgcgatggcg agatatttct gcgcatcttc cgcagtacgc acgccgcccg ccggtttgaa
    34501 accaacggtt ttttctacgc ccatatcacg gatcacttcc atcatgatgc gagcgctttc
    34561 cggcgtcgcg ttcacagcca ctttaccggt agaggttttg atgaagtccg cacccgcttt
    34621 gatggagatt tcagacgctt tacggatcag cgcttcgtct ttcagttcgc cggtttcgat
    34681 gatcactttc agcagtacat tcgctgccgc gcaagcctct ttacaggctt tcaccaggtc
    34741 aaaaccaacc tgctcgtcac ccgccatcag cgcgcggtac gggaacacca cgtcaacttc
    34801 atcagcaccg taggcgattg ccgcacgggt ttctgccagc gcgatttcga tgtcgtcgtt
    34861 accgtgtggg aagttggtta ccgtagcgat acggatttcc ggggtgccct gctctttcag
    34921 agttttgcga gcaatcggga taaaacgagg atagatacag atagcggcgg tattgccgac
    34981 cggagttttg gcctgatgac acagagcaat tactttctcg tcggtgtcgt cgtcattcag
    35041 ggtggtcagg tccatcaatt tcagtgcacg caggctgctt gctttcagat cagtcatttc
    35101 attctcctgg cttgtcgcca ataaaattca ccttgcgagt ttgttagtat tctaacatct
    35161 actccgcaac acacttcgat acacatcaca attaaggaaa tctacttaca agtttgcatc
    35221 actgtaatgc gatctggttc aaataattca ctttcaaatg aatgcgtcag tggtggcaaa
    35281 cgcatcagga tcaaagtgaa catcacgaaa cttcttacaa tggcgcatca ccttcggcat
    35341 aaaaggaaaa gaagatgccc acttctcatg aaaatgcact gcaacaacgt tgccagcaaa
    35401 ttgtcaccag cccagtgctt agcccggagc agaagcgcca ttttctggca ctggaagcag
    35461 aaaacaatct gccttaccca cagcttcctg ccgaagcccg ccgcgcgctg gatgaaggtg
    35521 taatctgcga tatgtttgaa ggtcatgcgc cgtacaaacc gcgctatgtc ttacccgatt
    35581 acgctcgttt tctggcgaac ggttccgaat ggctggagct ggaaggcgcg aaagatcttg
    35641 atgacgcact ctctctgctg accattcttt accaccacgt accgtcggtc acatcgatgc
    35701 cggtctacct ggggcaactg gatgcgttgt tgcaaccgta tgttagaatt ctaacacaaa
    35761 acgagatcga tgttcgaata aaacgtttct ggcgttacct cgacagaacc ctgccagatg
    35821 cctttatgca cgccaatatc ggcccgtctg attcgcccat tacccgcgcg atcttacgtg
    35881 cagacgcgca ggtttcaccg aacctgacct ttatctacga tcctgaaatc acccctgatg
    35941 acctgctgct ggaagtggcg aagaacatct gtgaatgtag caaaccgcac atcgccaacg
    36001 gtccggtgca tgataaaatt ttcacaaaag ggggctacgg gattgtgagc tgttacaact
    36061 cactgccgct ggcgggtggt ggcagcacgc tggtacgcct taacctgaaa gccattgccg
    36121 agcgcagcga atcgctggat gacttcttta cgcgcactct accgcactac tgccagcagc
    36181 agatcgccat catcgatgcg cggtgtgaat tcctctatca acaatcacac ttctttgaga
    36241 atagcttcct ggtgaaagaa gggctgatta accctgaacg ttttgtgcca atgtttggca
    36301 tgtatgggct ggcggaagcg gttaacttgc tgtgtgagaa agaagggatt gccgcgcgct
    36361 acggtaaaga agccgccgca aatgaagtgg gttatcgcat cagcgcgcaa ctggcggagt
    36421 ttgtcgccaa tacccccgtg aaatatggct ggcaaaaacg cgccatgtta cacgcacagt
    36481 cggggatcag ttccgatatc ggcaccacgc cgggcgcgcg tttgccgtat ggcgatgagc
    36541 cagatccgat cacccatctg caaactgtcg ccccgcatca tgcttattat tattccggca
    36601 tcagcgacat tctgacgctc gacgaaacca tcaaacgtaa cccgcaggca ctggtacagc
    36661 tttgcctcgg tgcctttaaa gccggaatgc gtgaatttac cgccaatgtc agcggtaacg
    36721 atctggttcg cgttaccggt tatatggtgc gtttgtcgga tttagaaaaa tatcgcgccg
    36781 aaggttcacg caccaacacc acctggctgg gcgaagaagc cgcacgcaac actcgtattc
    36841 tggaacgcca gccgcgcgtg ataagccatg aacagcagat gcgctttagt cagtaagatt
    36901 atccccttct cctgcgttga cgggccaggc agtcgtctgg ctctgttttt gcagggctgc
    36961 aatctgcgct gcaaaaactg tcacaatccg tggacgatgg gacgttgcaa tgactgtggg
    37021 gagtgcgtgc cacagtgtcc gcatcaggcg ttgcagattg ttgacggcaa agtggtgtgg
    37081 aacgctgtgg tctgcgagca atgtgatacc tgcctgaaga tgtgtccgca acatgccaca
    37141 ccaatggcgc aatccatgag cgtggacgaa gtgcttagcc atgtgcgcaa agcggtgctg
    37201 tttattgaag ggataacggt aagcggcggc gaagccacga cccaactgcc gtttgtagtg
    37261 gcgctgttta ctgctatcaa aaacgatccg caactgcgcc atctcacctg tctggtggac
    37321 agtaacggta tgttgagcga aaccggctgg gaaaaattac tcccggtgtg tgacggcgca
    37381 atgctcgatc tcaaagcatg gggtagcgaa tgtcatcaac aattaaccgg acgcgataat
    37441 caacagatta agcgcagcat ctatttgctg gcagagcgcg gcaagctggc ggaactgcgt
    37501 ttgctgatga tcccaggcca ggtggattat ttgcaacaca tcgaagaact ggcggcgttt
    37561 atcaagggac ttggtgatgt tccggttcgc ctgaacgcgt ttcatgccca cggcgtgtat
    37621 ggcgaggcgc aaagctgggc gagcgccacg ccggaagacg ttgagccgtt ggctgatgca
    37681 ttaaaggtgc gcggggtgag ccggttgata tttccggcgc tctatttgtg acatgccgga
    37741 tacggcctac ggttcggcac agacttgtag gcctgataag acgcgtcaag cgtcgcatca
    37801 ggcattgtgc gccaactgcc ggatgcggcg tgaacgcctt atccggccta cggttcggca
    37861 caaacctgta ggcatgataa gacgcgttaa gcgtcgcatc aggcattatg cgccgactgc
    37921 cggatgctgc ctacgaaggg caaactaccc acgaacgtta aacaccgcat acgtgttatt
    37981 aagcaacact tcggcaatct catccgcggg ttccgggcgt aactcgcaaa gcacggcaaa
    38041 cacacgggca gcctgttccg ggcggttagg ctgcccctga aaaccgttga gcggcatatc
    38101 cggcgcatcg gtttccagca acaacgatgc cagcggtaat tttgcgatga catcgcgggt
    38161 tttactggcg cgtggatagg tgatagtacc gcctacgccg attttgtagc ccagctgtac
    38221 aaaccgttca gcctgttgct ggctgccaga aaatccgtgg acaacaccag cacgcggcaa
    38281 atcgtggcgt ttaagatgca tcgccagttt gtcgtgcgtg cgccgtgaat gcaggatcac
    38341 cggcagatcg tagcgtttcg ccagtttcag ttgttcgtcg agtaaccact gctgcctctc
    38401 aaattgcgga tcgtcgccga agagatccag accgatctcc cccaccgcca ccaccttcgc
    38461 cggacgcctt tccagcgcct gctgtagctg ctcaagagac acatcgctat gtttttccaa
    38521 cataccagga tgcaagccca atacggcata cagcggttga taattttccg ctaacgcctg
    38581 cacacgggca aaattttccg cctctgtcgc cggaacaatg atcttgccta cgcccgcttg
    38641 tgccgcgcgt tgcaggctgg cctcttcatc gccaataaaa ggcgggaaat caaaatggca
    38701 gtgagtgtcg ataaaacggc aaatcaagcc agatcctcat tgttaaatgt cgggtcgttc
    38761 gcctgcggag cgtcgctcac ttcggcaacc agcgtatcgt tggcgactgg cgcaggcgga
    38821 atgacaatcg attccggcgt caccactggc accagatggc gggtaagcgg cgctttttca
    38881 gttaatagct tgccaaccgt ggcgaggaaa taacgaccgc ataaacgccc aagtttatag
    38941 tcttcacgca gcgccggaat ccgactgcca agcgcgatac tatgtaatgg cttcggcgga
    39001 taaatttcgg atatccgcag cttgcccggt ggtttctcaa taaattgctg aatgtcacga
    39061 tagctggttt catgatgctg caccagattg accagcggct gcaggctact gtcacccagc
    39121 cagcgttcca tgcgtttgaa ccactgcggc gtgtagtaca tttgtgacgg cacagtgcgg
    39181 atgacgacca acgttttagc gccctgcctt gccgcctctt taaccggaat cgcatcactg
    39241 atcccgccat ccaggtagtt aatgccttcc agcgacactc cgctacgata aaagccaggt
    39301 atcgcactgg aggcgcgaat cacatccagc cagttttgtt tggttggtaa aaagtaattc
    39361 ggcgcgtagt catcctgacg acaggcgcac atataaaacg atttgccgct gtcaaacaac
    39421 cgcgcggcgg tatccatttg caacggcatc tggcttgcgg tggcctccac cagccagtcg
    39481 agatcgataa gatttcctcc acggacaaag cgcaatggat cgaaaaattc gcgttttgtg
    39541 gtatagcgca tgatgacttt gcgcgcgtaa ccgggctgat tgcagataaa cgccgagagg
    39601 ttctgcgccc cggcagatgt gccgagataa agatcgaaag gattaaactg cgcgcgcata
    39661 aactcatcca gcacgccagc cgtgaaaatt ccacgctgtc cgccgccttc gcacaccaga
    39721 gctattcgtc caggctggaa cggccttagc gacaacggcg caatattacc aagcgttaca
    39781 ggtattcgct gccccacttc tgccttcctg tttttgttgt gaaagaaaga caaagtaacg
    39841 caatttatta ttcgctaaaa ccgcaaaagc cagtccgctg gactggcttt ggataaaatt
    39901 ttcttaatgg tctgaaatct agggtcgttt tcggcccatg aacaaactca ctaggaacag
    39961 aataatcccg acgacaaaga caattttagc tgcgcctgca gcggtaccgg ccagaccacc
    40021 aaacccaagt gcggcggcga ttaacgcgat aaccagaaat atgatgcccc aacgaaacat
    40081 aagcgtctcc tttaccatag ttaatgtcac cgcttgatat gagcgtgaaa atcactcaat
    40141 gcgatatttt tagtgtggtg cacatcgcgc ctcccgacaa agttcgggag gacgaattac
    40201 ttagttttca gatcattttt aacgcttttc acaccatcta ccgctttggc gatactttca
    40261 gcacggtcac tttgtgcctg agaatctacg gtaccggaga gctgaaccac gccatcggtg
    40321 gtttcaactt tcactttacg tgaagggacg atatcgtccg ccagcagttt ggctttgatt
    40381 tcactggtgg tggcggtgtc acccgcgtag cccttcaccg agccttcttt agcgtcgcga
    40441 acgtgcagtt tgtcgctgac agaggtcacg ccttcaacgc ctttcgccac tttcactgcc
    40501 tcttcggcct gggcctgact ttcaacgaaa ccgctcaggg tcacgacttt ttgatcggtt
    40561 ttgacagaga tatcggtgct cttgatgttg tcatgatcca ccagcgccgc cttcactttc
    40621 gcggtgatgg cgctgtcatc catgaaatta ccgactttat tcatagagct atcgactttt
    40681 tgccctgcgc tttcattggt agtctgcgcg ttgttttccg cgtaggcaga gccggtcgcg
    40741 acggcagagg tcaacattac agccagcaga gttttcgaaa tcttcagtct tgtcatagtc
    40801 atcgatttat tcctgtatgt ttgctcgtaa tttgagccta gcaacacgag gttgcattgc
    40861 tgaataggga gagacttcac cctctacaga agtcaatggt cgccatcaca aaagcgatgg
    40921 gtgatgaata acgaccatta cagcctctga atcagttatt aatatcggta gaatgacaat
    40981 cgacggcttt agatactaat atctacgcat tgaacggtat ttaacgccat cagaaatgtc
    41041 atcactttgt taaatataga tcacaatttt gaaaccgctc gggatatcag cgaaaacata
    41101 agcaaaagtg aatgttttaa gaacattccg taagcggcta ataaggaagg gaaattgaca
    41161 gggcgcagcg gctaccgcgc cctggaggca agaattaatg ctcgcgggtc tggtggaact
    41221 gaacgtccgg ataacgttcc tgtgccaggc gcaggttgac catgctggta gcgatgtaag
    41281 cgaggttatc gccgccatca agcgccagtt ggctttcgtt cttacgcttg aactcttcga
    41341 atttcttcgc gtctgcacat tctacccagc gggcagtggc aacgttgact gactcataca
    41401 ctgcttcaac gttgtattcg ctcttcaggc gcgctaccac cacatcaaac tgcagcacac
    41461 caaccgcacc aacgatcaga tcgttgttgg agattggacg gaacacctgc accgcgccct
    41521 cttcggaaag ctgtaccagc cctttgagca gctgtttttg cttcagcgga tctttcaggc
    41581 ggatacgacg gaacagttct ggtgcgaagt tcggaatacc ggtgaacttc atcatctcac
    41641 cctgggtaaa ggtgtcgccg atctgaatgg tgccgtggtt gtgcaggccg aggatatcgc
    41701 ccggatacgc ttcttcaacg tgcgaacggt cacccgccat aaaggtcagc gcgtcggaga
    41761 tcaccacatc tttcgcagtg cgcacctggc gcagtttcat gcctttttca tatttaccgg
    41821 acaccacacg cataaacgcc acgcggtcgc ggtgtttcgg atccatgttg gcctgaattt
    41881 taaatacgaa gccggtaaat ttatcttcgc tcgcttctac ggtacgggta tcggtctgac
    41941 gcggcatcgg cgcaggcgcc cactccacca ggccattcag catatgatca acgccgaagt
    42001 tacccagcgc agtaccgaag aataccggag tgatttcgcc cgcaaggaac agctctttgt
    42061 cgaactcgtt agacgcgcct ttaaccagtt ccagttcgtc acgcagctgc tgtgccagat
    42121 cttcaccaac cgcagcatcg agatccgggt tattcagccc tttaacaatg cggacttcct
    42181 gaatggtgtg ccctttaccg ctctgataga gataggtttc atctttataa aggtggtaaa
    42241 cgcctttaaa cagcttgccg cagccaattg gccaagtgat cggtgcgcag ccaattttca
    42301 gctcgttctc aacttcatcg agcagttcca tcgggtcgcg gatatcacgg tcaagtttgt
    42361 tcataaaggt gaggatcggc gtgtcgcgca gacgggtaac ttccatcagc ttacgggtac
    42421 gatcttcaac accttttgcg gcgtcgataa ccatcaggca gcagtccacc gccgtcaggg
    42481 tacgataggt atcttccgag aagtcttcgt gccccggggt gtcgagcagg ttaaccaggc
    42541 aatcgtgata cggaaactgc atcacagacg tagtaatgga gatcccacgc tgcttttcca
    42601 tctccatcca gtccgactta gcgtgctggt tggaaccacg gccttttact gtaccggcgg
    42661 tctgaatggc ctgtccgaac agcagcacct tctcggtgat ggtagtttta ccggcgtccg
    42721 ggtgagaaat aatggcaaaa gtgcggcgct tcgccacctc tttcaaataa ggagacaacg
    42781 tcataattct ttcttcttga gtaagcggca tcgcgccgcg cttgttgaaa acgaaaaatt
    42841 gcggctattt tacccatcaa cgggggggag gcaatcagtg tttacacagg agctgctcca
    42901 gttcgtgcaa cgaagaaacg gtccaggtgg gcgcgatgcc ttctggttgc tcgcgattgt
    42961 gcgcattcag ccagcaggtc gcaagcccgg cgttgatgcc accgagaata tcggactcgg
    43021 cagtgtcgcc aaccatcagc acgcgtgaac ggtcaggatt gcccgcctgt tccagcgcat
    43081 aatcgaaaat tttcttattc ggtttggcaa cgccaacttc ttcggaaatc accagcaaat
    43141 cgaagtaatc acgcaggccc gtccgttcca gacgcacttg ctgcaaggcg ctaaaaccgt
    43201 tggtgatgat gccgatttta gcgttgctac gaatggcgtt aagcagagaa accgcgcccg
    43261 gcaacggcgt acagatttcc gccatcgcat taataaaggc atcgttaagc aagcctgctt
    43321 caactttcag ccgttcggcc cagctctcga aacgcccgtg ctgaagctgt aatgaagtga
    43381 tcgcgccgtt ttgataatcc acccacagtg gcttgttaac ggcctgatag tcctgaaaat
    43441 cttcagcggt aaaggtgacg ctgtaatcaa gaaacatccg ctgcaggccg gtgaatgagt
    43501 caaaggtaaa cagcgtttca tcggcatcaa agaaaatcca gtcccacttc attattccac
    43561 cttgtcttac atactgattg gcaacgccat gatgatggcg tcttcgcgac cgtccgtggt
    43621 ggggtagtaa ttgcggcgaa tcgtcgcctc gttaaagcct aaactttcgt acagggcaat
    43681 ggcggcagca tttgaggcac ggacttccag ccatagtgtc gccacgccgc gtttttccag
    43741 ttcgtcgatc agatgttcca gcagcgccct acccagcccc tgacgctgat agtcgggatc
    43801 gaccgcaata ttgaacaatg tagcttcatc cagcaccact tgcgtaatcg caaacgccgc
    43861 cattttgccg ttttgcgtta actgaaagtt gagataacgc tcgccctggt tgctggcaaa
    43921 ggttttttca ctccacggaa aggcgtgggc gcgttgttca atgtggtaag ccgctggtaa
    43981 atcagtcgtt tcgagggaag aaatcgtgtt catatgtgca aatttgttgc cataacgcgg
    44041 cgcgtgccgt tgggtttgcc cgtaattcgg tgagcgccgg tgatgccacc tgagcgcctt
    44101 ccaatgatag cggttcgtca gtacccaacc gccagctgtt gcagcgactg ccttgcggca
    44161 gcatcgcgat tttttctggc gtcagttgca gcacttgatc ggggctgacg gttaatgcgc
    44221 gcagaacatc gctcactaaa ggatcagtca gggcgggaag atcgtttgcc accatcacca
    44281 gacggacgtg tgccgggctc gcaatggcaa tctccccctg caacgcgcca gggcgacgca
    44341 gcgaccactg ggtaatgccc agttgctgta actgccagtc tcgtcgggat gtcatagcga
    44401 atcgctcctg ttgtcagggg cgcaaatata gcaaattcgt cgataccgcg ccaacatatg
    44461 gctataatcg ccgccagtat caattgagga gcattccatg tctgcattta ccccggcaag
    44521 tgaagtcttg ctgcgtcaca gtgatgattt cgaacaaagc cgtattctgt ttgccggaga
    44581 cttacaggat gacctgcccg cgcgtttaga taccgcggcc agccgtgctc atacccagca
    44641 attccaccat tggcaggtgt taagctgcca gatgggggat aacgcccgtt ttagtctggt
    44701 cgccacggcg aatgatgtcg cagattgcga tacgctgatt tactactggc cgaagaacaa
    44761 accggaagcc cagttccagt tgatgaattt actttctctg ctgccagtgg ggacagatat
    44821 ttttgtcgtt ggcgagaacc gcagcggcgt gcgcagcgct gagcagatgc tggcagatta
    44881 tgcgccgttg aataaagtcg acagcgctcg tcgctgtggc ctctattttg gtcgtctgga
    44941 aaaacagcca gtatttgatg cggataaatt ctggggcgaa tacagcgtcg atggcctgac
    45001 ggtcaaaacg ctgcctggcg tgtttagccg cgacggtctg gatgtcggta gccagttgct
    45061 gctctcgacg ttaactccgc acacgaaagg taaagtgctg gatgtcggct gtggcgcggg
    45121 tgtactttca gttgcctttg cgcgccattc gccgaaaatt cgtctcacct tgtgcgatgt
    45181 ctctgccccg gcggtagaag ccagccgcgc aacacttgca gccaacggtg ttgaaggtga
    45241 agtctttgcc agcaacgtct tttccgaggt gaaaggttgt tttgatatga tcatctccaa
    45301 cccgccgttc cacgatggga tgcaaaccag cctggatgcg gcgcaaacgc tgattcgtgg
    45361 cgcggtgcgt catcttaata gcggcggcga gctgcgaatt gtagcgaacg ccttcctgcc
    45421 ttacccggac gtgctggatg agacatttgg cttccatgaa gttatcgcgc aaaccgggcg
    45481 cttcaaggta tatcgcgcca ttatgacccg ccaggcgaag aaaggttgag tatctgatat
    45541 ctgtcgccgc ataccgtgtc ggatgcggcg tgaacgcctt atccgaccta caaaacctgc
    45601 acgttagccc ttcgtaggcc agataagacg cgccagcgtc gcatctggca tctgcccccg
    45661 catgcctacc aaacctgcac gctagtttcc tgatggacat ttttccagca attacacatc
    45721 aggcgataat taactattga cgaaaagctg aaaaccacta gaatgcgcct ccgtggtagc
    45781 aattcttttt aagaattgat ggtatgcgaa ggtggcggaa ttggtagacg cgctagcttc
    45841 aggtgttagt gtccttacgg acgtgggggt tcaagtcccc cccctcgcac caattatctt
    45901 tacttccttt cttgtttctt ccctgttttg tcatccctga ttcaaattca ttatcatcag
    45961 catgatgcca gcaataagcg ccaggcacga tggtagcaaa atgtaatgcc gcaactgttt
    46021 gtgatacagc atcaccggcg tcaatactaa tcccatcaga atgacaatcc gccactgatc
    46081 cggcgataaa tccgcataca tcagccctgc aatcagcaat cccggcagca acactcccca
    46141 gccactgccc agcgtacgtt gcaacatggt ttcatctctt tcattgataa tgaaaaccaa
    46201 tatcatatga taatttttat catttgcaag ccagagaaat cccttgctat cgagtaaacc
    46261 tatcgctagg attagcaatc attatcattt agattactat cacgattatg gcctatcgtt
    46321 ccgcaccgct ctatgaagat gtcatctggc gaacgcatct ccagccgcag gatgctgggc
    46381 ttgcacaagc cgtacgggcg atgatcgcca aacatcgtga acatttgctg gagtttatcc
    46441 gcctggatga acccgctccg cttaacgcca tgacgctggc gcaatggtca tcaccgaatg
    46501 cgctaagttc tctactggcg gtctattccg atcatatcta tcgcaaccaa ccaacgatga
    46561 tccgcgagaa caaaccgctg atctcactat gggcacaatg gtatatcggc ctgatggtgc
    46621 caccattaat gctggcgcta ctgacgcagg aaaaagcatt agatgtgtcg ccggaacatt
    46681 tccatgctga gtttcacgaa accggacgcg tcgcctgttt ctgggtcgat gtgtgtgaag
    46741 ataaaaacgc aacaccacat tcgccgcagc agcgaatgga aacgttaatc agccaggcgc
    46801 tggttccggt tgtgcaagca ctagaagcga ccggagagat caacggcaaa cttatctgga
    46861 gtaataccgg ttatttgatc aactggtatc tcactgagat gaagcaactg ctcggcgagg
    46921 ctactgttga atcgctgcgc catgccctct tttttgagaa aacgctcacg aacggtgaag
    46981 ataatccact ctggcgtacc gtggtactgc gcgacggcct gctggtgcgc cgcacttgct
    47041 gccagcgtta tcgcttaccg gatgtgcagc aatgtggcta ttgtacgctg aaatgaaagc
    47101 actgccgggg aagtaaaccc ggcatcatgc ggattaatag ggatgcaaca cattacttgc
    47161 ttcgcaatgc cgcatacata acagaatatc ttcggcctcc aacaaccctg catcagaact
    47221 gacgcccagt ttcgacatca cattaaattt atgcgctcgg atcgttttga tattacgttt
    47281 aagctgctcg gcaatttgtg tcattgagta gccacgcgac ataaagcgca atatttctct
    47341 ctccgtcggg cttaacgtcc ggctttggtt aatgtaccac tgattgttca gtcggtcggt
    47401 cgcctgacgt acaccattta atgacaaaaa gagttcctga tgaaaaatct ccagcgttga
    47461 cgctttactt aatacaccgt ccagcggtga tggcgacaat gaaccaatca gccgagcttc
    47521 aatatcatca tccacaataa ctaaacgccg ggtacgcgga aacttaatcg ccagctcagt
    47581 caggcaagat aatccctctc tgcgctcact tctcatggca gaaaaagaaa aaatgaccgc
    47641 cgcaaacgag atccgcgaca tggcatcctg aaatgactcc tgactggtat aggtatggag
    47701 cttataaggg ttgcccgcaa actttctgaa taaactctca ataccgatac tactcatgac
    47761 gcatttttct atgagtgcga catttctctt cttaattcgg ctgtgttcca ttctgcggca
    47821 tctccataaa cagaaggcaa gttaagttct cttgcaccct gcgcgctatt tatccatgca
    47881 tacatttcag cgttgctgtg aattgccagt cgacgcatcg cactgttttt ttgggcgctg
    47941 atagttttat tacttttctt aagcaatgaa gctatctggt tgatgcccca tcctttacct
    48001 aatagccgca gtaccttgcg ctctgaaaga gtgagaatga cggagcgata gctaaaatca
    48061 tgagtttcag tcattgccgg agaggtcagc atggcgctga tacgctctgc gtgcgtattg
    48121 ccggaacgta cggtcttgac cagattctca atgggttcaa catcagacaa taacgtcgcc
    48181 gtagggcaca tgagcagttc tacagcctgg gaataccagg agcgtgacac catgaaaacc
    48241 cagtgaattt cccgatattg tgagattaaa gaataataat gttcacaaac agaacgtgga
    48301 tcttcacttt caccggctaa atcagcaatg act
//
LOCUS       DAZPCS010000015        47664 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZPCS010000015 DAZPCS010000000
VERSION     DAZPCS010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47664
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.015"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            75..227
                     /gene="hokA"
                     /locus_tag="ACHUQ7_000816"
     CDS             75..227
                     /gene="hokA"
                     /locus_tag="ACHUQ7_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFR1134345.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     AYKLKE"
     gene            complement(204..275)
                     /gene="ysaE"
                     /locus_tag="ACHUQ7_000817"
     CDS             complement(204..275)
                     /gene="ysaE"
                     /locus_tag="ACHUQ7_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsaE"
                     /protein_id="HFR1134346.1"
                     /translation="MRNAVSKAGIISRRRLLLFQFVG"
     gene            complement(414..626)
                     /gene="cspA"
                     /locus_tag="ACHUQ7_000818"
     CDS             complement(414..626)
                     /gene="cspA"
                     /locus_tag="ACHUQ7_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFR1134347.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(907..1197)
                     /locus_tag="ACHUQ7_000819"
                     /pseudo
     CDS             complement(907..1197)
                     /locus_tag="ACHUQ7_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
     gene            1631..2341
                     /locus_tag="ACHUQ7_000820"
     CDS             1631..2341
                     /locus_tag="ACHUQ7_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFR1134348.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2391..3365)
                     /gene="ghrB"
                     /locus_tag="ACHUQ7_000821"
     CDS             complement(2391..3365)
                     /gene="ghrB"
                     /locus_tag="ACHUQ7_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFR1134349.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRVHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3469..4129)
                     /locus_tag="ACHUQ7_000822"
                     /pseudo
     CDS             complement(3469..4129)
                     /locus_tag="ACHUQ7_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
     gene            4282..6480
                     /locus_tag="ACHUQ7_000823"
     CDS             4282..6480
                     /locus_tag="ACHUQ7_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HFR1134350.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALAGGYTGHLGDY
                     STGAAQAIMPYVVGGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGL
                     SYFSALRDSGKKLICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWH
                     DEAFLARCTTGYAVFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTML
                     MAGWGMQRQQFGEQKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGDPTRRSAVLSSM
                     QGSLPGGCDAVDKIPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQD
                     TNRLIRAWQKPELVVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMK
                     QVVPPRYEARNDFDVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVE
                     LPPFAEFWQANQLIEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYG
                     YPDCPGHPMWLEPDEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVT
                     IHPDDAQARGITEGDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGI
                     CKNGAVNVLTKDLPSSRLGNGCAGNTTLAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6449..6889)
                     /locus_tag="ACHUQ7_000824"
     CDS             complement(6449..6889)
                     /locus_tag="ACHUQ7_000824"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFR1134351.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(6886..7449)
                     /gene="tag"
                     /locus_tag="ACHUQ7_000825"
     CDS             complement(6886..7449)
                     /gene="tag"
                     /locus_tag="ACHUQ7_000825"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFR1134352.1"
                     /translation="MERCGWVSQGPLYIAYHDNEWGVPETDSKKLFEMICFEGQQAGL
                     SWITVLKKRENYRAYFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYLGNKP"
     gene            7607..8305
                     /locus_tag="ACHUQ7_000826"
     CDS             7607..8305
                     /locus_tag="ACHUQ7_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFR1134353.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8534..9742
                     /locus_tag="ACHUQ7_000827"
     CDS             8534..9742
                     /locus_tag="ACHUQ7_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1134354.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTRSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10066..11757
                     /gene="eptB"
                     /locus_tag="ACHUQ7_000828"
     CDS             10066..11757
                     /gene="eptB"
                     /locus_tag="ACHUQ7_000828"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFR1134355.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LVVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            11849..11925
                     /locus_tag="ACHUQ7_000829"
     tRNA            11849..11925
                     /locus_tag="ACHUQ7_000829"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11883..11885,aa:Pro,seq:cgg)
     gene            12669..14276
                     /gene="dppA"
                     /locus_tag="ACHUQ7_000830"
     CDS             12669..14276
                     /gene="dppA"
                     /locus_tag="ACHUQ7_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFR1134356.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            14584..15603
                     /gene="dppB"
                     /locus_tag="ACHUQ7_000831"
     CDS             14584..15603
                     /gene="dppB"
                     /locus_tag="ACHUQ7_000831"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFR1134357.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            15613..16515
                     /gene="dppC"
                     /locus_tag="ACHUQ7_000832"
     CDS             15613..16515
                     /gene="dppC"
                     /locus_tag="ACHUQ7_000832"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFR1134358.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            16526..17509
                     /gene="dppD"
                     /locus_tag="ACHUQ7_000833"
     CDS             16526..17509
                     /gene="dppD"
                     /locus_tag="ACHUQ7_000833"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFR1134359.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            17506..18510
                     /gene="dppF"
                     /locus_tag="ACHUQ7_000834"
     CDS             17506..18510
                     /gene="dppF"
                     /locus_tag="ACHUQ7_000834"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFR1134360.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(18540..19811)
                     /locus_tag="ACHUQ7_000835"
     CDS             complement(18540..19811)
                     /locus_tag="ACHUQ7_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFR1134361.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPVTWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            20287..20394
                     /locus_tag="ACHUQ7_000836"
     CDS             20287..20394
                     /locus_tag="ACHUQ7_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR1134362.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            20770..20877
                     /gene="ldrD"
                     /locus_tag="ACHUQ7_000837"
     CDS             20770..20877
                     /gene="ldrD"
                     /locus_tag="ACHUQ7_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxic polypeptide
                     LdrD"
                     /protein_id="HFR1134363.1"
                     /translation="MTFAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            21253..21360
                     /locus_tag="ACHUQ7_000838"
     CDS             21253..21360
                     /locus_tag="ACHUQ7_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR1134364.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            21736..21843
                     /locus_tag="ACHUQ7_000839"
     CDS             21736..21843
                     /locus_tag="ACHUQ7_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR1134365.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(21930..23609)
                     /gene="bcsG"
                     /locus_tag="ACHUQ7_000840"
     CDS             complement(21930..23609)
                     /gene="bcsG"
                     /locus_tag="ACHUQ7_000840"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFR1134366.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDIWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIDFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(23606..23797)
                     /gene="bcsF"
                     /locus_tag="ACHUQ7_000841"
     CDS             complement(23606..23797)
                     /gene="bcsF"
                     /locus_tag="ACHUQ7_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFR1134367.1"
                     /translation="MMTISDIIEIIIVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(23794..25365)
                     /gene="bcsE"
                     /locus_tag="ACHUQ7_000842"
     CDS             complement(23794..25365)
                     /gene="bcsE"
                     /locus_tag="ACHUQ7_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFR1134368.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            25638..25826
                     /gene="bcsR"
                     /locus_tag="ACHUQ7_000843"
     CDS             25638..25826
                     /gene="bcsR"
                     /locus_tag="ACHUQ7_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFR1134369.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            25838..26590
                     /gene="bcsQ"
                     /locus_tag="ACHUQ7_000844"
     CDS             25838..26590
                     /gene="bcsQ"
                     /locus_tag="ACHUQ7_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HFR1134370.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            26587..29204
                     /gene="bcsA"
                     /locus_tag="ACHUQ7_000845"
                     /pseudo
     CDS             26587..29204
                     /gene="bcsA"
                     /locus_tag="ACHUQ7_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            29215..31554
                     /gene="bcsB"
                     /locus_tag="ACHUQ7_000846"
     CDS             29215..31554
                     /gene="bcsB"
                     /locus_tag="ACHUQ7_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFR1134371.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMSGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNFEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGTPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLATISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            31561..32667
                     /gene="bcsZ"
                     /locus_tag="ACHUQ7_000847"
     CDS             31561..32667
                     /gene="bcsZ"
                     /locus_tag="ACHUQ7_000847"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFR1134372.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            32862..33251
                     /locus_tag="ACHUQ7_000848"
     CDS             32862..33251
                     /locus_tag="ACHUQ7_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001391818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase subunit BcsC-related outer
                     membrane protein"
                     /protein_id="HFR1134373.1"
                     /translation="MISNAACKTTVWHSRQRYWKTRANGRRRQHFSGNDWRWAPAAYG
                     LLTDWQEEAARQSNDGGSSQGFGYTARALLERRVTSNWFVGTAIDIQQVKDYAPSHFL
                     LYVRYSAAGWQGDMDLPPQPLIPYADW"
     gene            33333..35321
                     /gene="hmsP"
                     /locus_tag="ACHUQ7_000849"
     CDS             33333..35321
                     /gene="hmsP"
                     /locus_tag="ACHUQ7_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFR1134374.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            35510..36796
                     /gene="dctA"
                     /locus_tag="ACHUQ7_000850"
     CDS             35510..36796
                     /gene="dctA"
                     /locus_tag="ACHUQ7_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFR1134375.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGALGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            37017..38513
                     /locus_tag="ACHUQ7_000851"
     CDS             37017..38513
                     /locus_tag="ACHUQ7_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFR1134376.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(38609..39538)
                     /gene="kdgK"
                     /locus_tag="ACHUQ7_000852"
     CDS             complement(38609..39538)
                     /gene="kdgK"
                     /locus_tag="ACHUQ7_000852"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFR1134377.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRAKG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEALVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            39770..40537
                     /gene="pdeH"
                     /locus_tag="ACHUQ7_000853"
     CDS             39770..40537
                     /gene="pdeH"
                     /locus_tag="ACHUQ7_000853"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFR1134378.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            40607..42667
                     /locus_tag="ACHUQ7_000854"
     CDS             40607..42667
                     /locus_tag="ACHUQ7_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFR1134379.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGNLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(42849..44171)
                     /locus_tag="ACHUQ7_000855"
     CDS             complement(42849..44171)
                     /locus_tag="ACHUQ7_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1134380.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(44582..45595)
                     /gene="yhjD"
                     /locus_tag="ACHUQ7_000856"
     CDS             complement(44582..45595)
                     /gene="yhjD"
                     /locus_tag="ACHUQ7_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFR1134381.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(45644..46543)
                     /locus_tag="ACHUQ7_000857"
     CDS             complement(45644..46543)
                     /locus_tag="ACHUQ7_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1134382.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            47063..>47664
                     /locus_tag="ACHUQ7_000858"
     CDS             47063..>47664
                     /locus_tag="ACHUQ7_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFR1134383.1"
                     /translation="MQIVMFDRQSIFIHGIKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT        11413 a        11878 c        12827 g        11546 t
ORIGIN      
        1 agtgaggcta ccctatgcct gaacaccata aggttagcct cttactcgtt ggaaatcaac
       61 acagggggct gggaatgccg cagaaatata gattactttc tttaatagtg atttgtttca
      121 cgcttttatt tttcacctgg atgataagag attcactgtg tgaattgcat attaaacagg
      181 agagttatga gctggcggcg tttttagcct acaaattgaa agagtaagag tcttcgacgg
      241 gaaattattc ccgccttact tacggcgttg cgcattctca ttgcacccat atttattctt
      301 cacaaaaata ataatagatt ttattacgcg atcgattatt tatttcctga aaaaaaataa
      361 aaaaatcccc gccaaatggc agggatctta gattctgtgc ttttaagcag agattacagg
      421 ctggttacgt taccagctgc cgggccttta gcgccgcttt cgatggtgaa ggacactttc
      481 tgaccttcgt ccagagattt gtaaccatcg ttctggatag cagagaagtg tacgaacaca
      541 tctttagagc catcgtcagg agtgatgaag ccgaagcctt tgtcagcgtt gaaccatttt
      601 acgataccag tcattttacc ggacatagtg tattaccttt aataattaag tgtgcctttc
      661 ggcgatatgg cgtgctttac agattttgaa gcgttaaagg aatgtgcact acgaggggta
      721 tcaacgataa ctcttgaagg gacttgcctt actacactgc tttaatggtc tgtacgtcaa
      781 accgttgatg tgcattaagc cactcattgg cgggtgatgc aacaattatt tttcatattt
      841 atgattaatc ggttggaaaa cggtcctgtc atcaggaccg taaacagcaa taaagtggat
      901 aaaagtctat ttcatcaact gcttacttaa tgccgggttg gcttgaatca aacgcatcaa
      961 ttttagttcg gcacttgaag gcttcacgcg tctggattcc cattccttta ccatggcgac
     1021 tgatacgccc aaaacccggg cgaaatcatc gatttttaat cctgtccctt ttcataactg
     1081 ctcaatttcg gtacaggacg ttgttctgtg cgtcagggta attttctgcg tttcatcttt
     1141 aaaaacaatc tgttccaggc tgctcaacag ctcatgcatt ggatctttat attccattga
     1201 aaactcctca aatcacactg cgggatcgtg aattgcatcg aagctcatta agaatagtcg
     1261 ggaaaagcag acacagcggg gtgaagtcag ggcatgctct ggctatgatt cgattcagcc
     1321 tgtcatttag cgtgacttat agccacctaa cgatctgata aatgccgata tatctcttat
     1381 cactcctgtt ttgtaaagat ttcgcaaatc taaccttctg cgaaggcgta aatctctggc
     1441 tacaaaaaag gcaacggcac tatcattttt taacaatgaa acaacaaatt aaccttgttt
     1501 cattttctcc atgattttac caggaagatt acgtagaaag ggtatcctgc aagcctgtcc
     1561 tggactatcc ttgtcacgtc agacacgcgt gtgtcgttgt gcgctttttt tgggtgaaag
     1621 gagtaagaaa atggcgacag gaaagtcctg ctctcgctgg tttgcgcctc ttgcggcgtt
     1681 attaatggta gttagcctga gtgggtgttt tgataaagaa ggcgatcagc gtaaagcgtt
     1741 tatcgacttc ctgcaaaata cagtgatgcg tagcggtgaa cgtctaccaa ccctgactgc
     1801 cgatcagaaa aaacagtttg gtccttttgt ctctgattac gcgattctgt atggttattc
     1861 tcagcaggtg aatcaggcga tggattccgg tctgcgcccg gtcgtagaca gcgttaacgc
     1921 aattcgcgta ccgcaggatt acgttacgca aagcggtccg ctgcgtgaaa tgaatggttc
     1981 gctgggtgtg ctggcgcaac aactgcaaaa tgcgaagttg caggccgatg ctgcgcactc
     2041 tgcgctaaaa cagagcgatg acctgaaacc ggtatttgat caggcattca ccaaagtggt
     2101 aacgacgcca gctgatgcat tgcaaccgtt gattccggcg gcacaaactt tcacgcaaca
     2161 actggtgatg gttggagact acattgctca gcaaggtact caggtgagct ttgtcgcaaa
     2221 tggcatccag ttcccgactt cacagcaggc aagtgaatat aacaaactga ttgcgccatt
     2281 accggcacaa catcaggcgt ttaatcaggc ctggactaca gcagttactg caacccaata
     2341 aagagtaaaa gcccgagcga ttatcgggct ttacttcacg cagtcgcagc ttagtccgcg
     2401 acgtgaggat tcacacagtt cttctcaacc tttccttgta acgcatcaat caaattatcc
     2461 acggcacagg cggccatgcc ataacgcgtc tcatgggtgg cagatccaat atgcggtact
     2521 gcgacgacgt tggccattga gagcaacggc gaatctacgg acagtggctc ttgttcgaag
     2581 acatccagcc cggcggcgtg aatttcgcct ttctgcaatg ctgcgatcag tgcattttcg
     2641 tcaaccaccg ggccacgtcc ggcattaatg aaaatggcgg aggatttcat tttggcgaat
     2701 tgttctgcgc caaacagatg atgcgtctca tcagttaacg gcaggatcag gcaaacgaaa
     2761 tctgactctt gtaacagagt atccaaatcg cagtagcggg cgttgaagcg ttcttctgct
     2821 tctttatggt ggcggcgcgc gttatagagg atgggcatgt tgaagccaaa gtgcacacgt
     2881 tgtgccagcg ccatgccgat ccgtcccatc ccgacaatgc ccagtgtttt atggtgaacg
     2941 tcagtgccgt accagtccgg gcctatgctc gcggtccatt cgcctgcttt tacccgttct
     3001 gctacctcca caacccgacg agcggtagac aacaccagcg ccatcagcgt atcggcgacg
     3061 gtttctgtta atacggttgg cgtgtgcatc agcagaattt ttcgggcggt aagcgcatcg
     3121 acatcaaaat tgtcatagcc gacggagatc gttgatgtgg cacgcagttt cggcattttt
     3181 tccagcaatg cggcatctac attctcgttt gaacccagta aaccttcagc ttctgcaaaa
     3241 attgctgcat tttgaacgac ggtttgtggg ctgaggtttg ccacctggtg aacggtgaaa
     3301 tgcgcttgca ggcgttgcag taaatcatca ggtaaggctt tgtagaggat aacggacggc
     3361 ttcatgcttc tctccattca ctgataaggc gatcacctta gcctgaaata tgatgaagga
     3421 aaactgaaga atgccaggtg atttctgcat cacctggcat gaaagggatt acagcgggct
     3481 taaggtaatt tctacacggc ggttttgcgc cttaccttct gcggtgctgt tgctggcgat
     3541 tgggttagcc gggccaaggc cctgagtacg gatgcggctg gcgtccacgc cctgggtgat
     3601 caacgcgctg gcaacggaat ccgcacgctg ctgggagaga cgcatgttca ggtcgtgacc
     3661 ccccgtgctg tcggtataac caatcacgtt aaccgccgtt ttcggatact ctttcagcac
     3721 cattgccacg ccggtcaggg tgttagcgcc tgccggtttc aggggagcgc tgctgctgtc
     3781 gaaggtcaca ttgttcggca tattgaggat aatgttatcc ccgctgcggg ttacgctaac
     3841 accagtgccg cgcattttgt cacgcagctt cgcttcctgc acatccatgt aataacccac
     3901 gccgccgccc agagctgcgc ctgctgctgc gccaatcagc gcgcctttac cgcgatcttt
     3961 cttcgaagaa gagagcgcac caatacccgc gcccacgaga gagcccagac ctgcgccgat
     4021 agcagatttg cctgcttcgc gttcgccggt gtaagggtta gttgtgcagc cagataccgc
     4081 cagagcgcca ctcactacgg cggcaagtaa gataaacacg tttcttcatt gttaatcctt
     4141 aataaccttt ttattctttg tcacgggttc cgtggcggga gattatgccg cgtgaacatg
     4201 aagattattc ctgggaatac tcggaaattt gtaagtaata tttaactgct caatacatct
     4261 aacctttcag gagtcttcgg tttggccaac tcatcctcac gatattccgt tctgactgcc
     4321 gcccactggg ggcccatgct ggttgaaacc gacggcgaaa ccgtgtttag ctcgcgtggc
     4381 gcgttagcca caggaatgga aaactccttg cagagcgcgg ttcgcgacca ggttcacagc
     4441 aatacgcggg tacgatttcc aatggtgcgc aaaggctttc tcgcgtcacc ggaaaacccg
     4501 caaggcattc gtgggcagga tgaatttgtt cgcgtgagtt gggatgaggc gctggcaggc
     4561 ggttataccg ggcatctggg ggattattcg accggtgcgg cacaggcgat catgccgtat
     4621 gtcgtaggtg gcagcgaagt ttatcaacag caaaccagtt ggccgctggt gctggaacat
     4681 agcgatgtcg tggtgttgtg gagtgctaac ccactcaata cgctgaaaat tgcgtggaat
     4741 gcatccgatg agcaggggct ttcttacttt tccgcactgc gtgacagtgg gaaaaagctg
     4801 atctgcattg atccaatgcg atcggaaacc gtcgatttct ttggcgataa aatggagtgg
     4861 gtggcaccgc acatgggcac cgatgttgcg ctgatgctgg ggatcgccca tacgctggtg
     4921 gaaaatggtt ggcacgacga agcgtttctg gcgcgttgca ccacaggtta tgccgtcttc
     4981 gcctcttatt tgctgggcga gagtgacgga atagcgaaaa acgccgaatg ggcggcagag
     5041 atttgtggtg ttggcgcagc gaaaatccgc gagctggcgg ctattttcca ccaaaatacc
     5101 accatgctga tggcaggctg gggaatgcag cgccaacagt ttggtgagca aaaacactgg
     5161 atgatcgtca cgctggcagc aatgttgggg caaatcggca cacccggcgg cggttttggt
     5221 ctttcttacc attttgccaa tggtggtgac cccacgcggc gttctgcggt gctctcttcc
     5281 atgcagggca gcttgccggg tggctgcgat gcggtggata aaatccctgt tgcccgcatt
     5341 gttgaagcac tggaaaaccc tggtggcgca tatcaacaca acggtatgaa ccgacatttc
     5401 ccggatattc gttttatctg gtgggcgggc ggtgccaact ttactcatca tcaggatacc
     5461 aatcgtctga tccgtgcctg gcaaaaaccg gagctggtgg tgatctctga atgcttctgg
     5521 acggcggcgg cgaaacacgc ggatattgtt ctgcctgcga ctacctcttt tgagcgtaat
     5581 gatctcacca tgaccggtga ttacagtaat cagcatctgg tgccgatgaa gcaagtggtg
     5641 ccgccacgct atgaagcgcg taatgatttt gatgtttttg ccgagttgag tgaacgttgg
     5701 gagaagggcg gctatgcgcg gtttacggaa ggaaaaagtc agctgcaatg gctggaaacg
     5761 ttttataacg ttgcccggca gcgcggggca agccagcagg ttgaattgcc gccatttgct
     5821 gagttctggc aagccaacca gttaattgag atgccggaaa acccggacag cgagcggttt
     5881 attcgcttcg ctgatttttg ccgcgatccg ctggcgcatc cgttaaaaac cgccagcggc
     5941 aagattgaaa tcttctcaca gcgtattgcc gattacggtt acccggattg ccctgggcat
     6001 ccaatgtggc tggagccgga cgaatggcag ggcaatgccg aaccagaaca gttgcaggta
     6061 ctttctgccc atccggcgca ccgcctgcac agccagctga attacagttc tctgcgcgaa
     6121 ttgtacgcgg tagcaaaccg tgagcctgtc accattcatc ctgacgatgc tcaggcgcgc
     6181 ggcataaccg aaggggatat ggtgcgggtg tggaactccc gtgggcagat ccttgccgga
     6241 gcggtcatta gcgagggaat taaacctggc gtgatttgca ttcacgaagg ggcatggccg
     6301 gatctggatt taaccgctga cggtatttgt aaaaacggcg cagtgaacgt gctgaccaaa
     6361 gatctcccca gctcgcggct ggggaatggc tgtgcgggta atacgacgct ggcatggctg
     6421 gaaaaataca acggtccgga actgacactt acagcgtttg aaccaccggc cagctcataa
     6481 tccaggtggg tagttgggtt tcatcctgcc atgcgcaatc gacaatgtga aaaccctgcg
     6541 cacggtaaaa atctatcgcc ggttgatttt tttgataaac ctccagcatc agatggggat
     6601 agcgctgctg cacatactgc atcagcgcct taccaatacc gcgtctgacg gccttcggtg
     6661 cgacaaacat tgctgccaga aatcggcctt ccataatgct gacaaaaccg agaagcttac
     6721 cgtcctcttc ccagacccag ttttgcgcgt tggcaagata ggcatcccgc accagcggaa
     6781 tgcagtcacg ccagtaattc gcttttataa agggatgccc ccaggttgta ctttccagcc
     6841 acagttcgag gatcgcgggg agttctgaac gttgcgcttc ccgaatcatg gtttatttcc
     6901 cagatagcaa cagcagccaa ccacatgatc attcaccagc ccacatgcct gcataaagga
     6961 gtaacagatt gtggtgccga caaacttaaa accacgtttt ttcagtgcct tagatagggc
     7021 gtcggaggcg gacgtagatg tgggaatttc gctcaacgtt gtggcttgtg tcacctgtgg
     7081 ctgatgattt acaaacgacc agacaaagtc gggaaacggt tcgccgttct gttccatttg
     7141 caggtacgcc cgcgcattac caataattgc ctgaattttc cctcgatggc ggataatccc
     7201 ggcgtcctgt accagtcttt cgacatcctc ttcctgcatt gctgcgacct tcaccggatc
     7261 gaactgatga aagtaggcgc gatagttttc gcgttttttg aggacggtga tccacgataa
     7321 cccagcctgc tgcccttcaa agcagatcat ttcgaacagt tttttactgt cagtttcagg
     7381 cacgccccac tcattatcat ggtaggcaat ataaagcggg ccctgactca cccagccgca
     7441 acgttccata ctttccctcg ctatgtaccc gatcgtaaaa atttcagccg cttttcttga
     7501 cgcatcggtg aaaaagtcaa tatttaatac agggctacta tgcccgatat ccttcataca
     7561 atgacaataa tattgccgaa ttcggctctt ccctggagtc gtgttaatga tcataaaaaa
     7621 aagcggtggt cgctggcagc taagcctgct ggcgagcgtg gtaatcagtg ccttttttct
     7681 caacacagct tacgcctggc aacaagaata tatcgttgat acgcaacccg gacattccac
     7741 agagcgttac acctgggata gtgatcatca acctgattac aacgatattt tgtcgcaacg
     7801 tattcaaagt agccaaaggg cgctgggact ggaagtcaat ctggcggaag aaactcctgt
     7861 ggatgtgacc agcagtatga gtatgggctg gaattttcct ttatatgaac aggttacaac
     7921 cggcccggtc gcggcattac attacgatgg cacaaccacc tcgatgtata acgagtttgg
     7981 cgacagtact accacgctga ccgatccgtt atggcatgcc agcgtgagta gcttaggctg
     8041 gcgtgttgac tcccggcttg gcgatctccg accctgggcg caaatcagct ataaccagca
     8101 atttggcgag aatatctgga aggcgcaatc aggcctgagc cggatgacgg cgacaaacca
     8161 gaacggcaac tggctggatg tcaccgtagg cgctgatatg ttgctcaatc aaaatattgc
     8221 cgcctatgct gcgctaactc aggcagaaaa taccactaat aatagcgact atctgtatac
     8281 gatgggggtt agcgccagat tttaacgtaa cagtcacaat tgaaaccatt gaataacaac
     8341 agttgtggcg atagtgggtg ctaacttacc aaataataaa tttggtgaat aattgtcgcg
     8401 tcattcattc ctgaactaag gcatttcatt ccgttctgat ggcatttcat gccgtttttc
     8461 cccaagcata aaatgcactt cgttatggtt gtcggcagag atttttcctt tttattactg
     8521 caggaatact gccatgacac cttcaaatta tcagcgtacc cgctggctga cactcatcgg
     8581 tactatcatt acccagtttg cgctggggtc ggtttatacc cggagcctgt ttaatggcgc
     8641 gctttctgcc aagctggatg cgccggtaag ccaggtcgct ttctctttcg gcttgttaag
     8701 tctggggctg gcaatttcgt cttctgttgc gggcaaattg caggaacgtt ttggcgttaa
     8761 acgcgtcacc gtggcttccg gcattttgct gggattaggc ttcttcctga ccgcgcattc
     8821 caacaacctg atgatgctgt ggttaagcgc cggtgtgctg gtcggtctgg cggatggcgc
     8881 gggttacctg ctgacgctct ctaactgcgt gaagtggttc ccggagcgta aaggtctgat
     8941 ctccgcgttc gctatcggtt cttatggtct gggtagcctg ggtttcaaat ttatcgacac
     9001 gcagctgctc gaaacggtgg gtctggaaaa aacctttgtg atttggggag cgattgcgct
     9061 ggtgatgatt gttttcggcg caacgttaat gaaagacgca ccaaaacagg aagtgaaaac
     9121 cagcaatggt gtggtggaga aagattacac gctggcagag tcgatgcgta aaccgcagta
     9181 ctggatgtta gcggtaatgt tcctgactgc ctgcatgagc ggcctgtacg tgattggggt
     9241 agcgaaagat atcgcccaaa gtctggcaca ccttgatgtg gtttccgcag ccaatgcagt
     9301 aacggttatt tccatcgcta acctttcagg tcgtctggtg ctgggcattc tgtctgacaa
     9361 aattgcccgt atccgtgtta tcaccattgg tcaggtgatt tcgctggtgg gtatggcggc
     9421 cctgctgttt gcaccattaa atgcagtgac gttctttgca gcgattgcct gcgtggcatt
     9481 taactttggc ggcactatta ccgtctttcc atcactggtc agtgagttct tcggcctcaa
     9541 taacctggcg aaaaactacg gtgtgattta cctcggtttc ggtatcggta gcatttttgg
     9601 ttcgattatc gcctcactgt ttggcggttt ctatgtgacc ttctacgtga ttttcgccct
     9661 gctgattctg tcattggcgc tttctacgac gattcgtcag ccagagcaga aaatgttgcg
     9721 tgaggcgcat ggctcccttt aatgctcagc cgcgagctac tgggcttcag cttccgtgtg
     9781 catcaaacgc atcaacgaaa tgtataaaca atgactcgta aaatttgggc cagttaactg
     9841 gcccttttta ctgctttttg taacgctaaa gtctcttttc aaacttgcat ttttgtaaat
     9901 ttgtgcttca tgcacactct ttccccacac tttttcgctt tgctgtggtc tacttattcg
     9961 cgcgtgtaga ttttacttat ctgactacct ccgcactttt cccctgccgg gcctgaaaag
    10021 ccgctaagca gggtgttatc acctgtttgt ccagggtttg tttgcatgag atacatcaaa
    10081 tcgattacac agcagaagct gagctttttg cttgcaatct atattggcct ttttatgaat
    10141 ggcgcggttt tttatcgccg cttcggcagc tatgcgcacg attttaccgt ctggaaaggc
    10201 gtttctgctg ttgttgaact ggccgccacc gtactggtga ccttcttttt actacgtctt
    10261 ctatcgctgt ttggccgccg cagctggcgt attctggcgt cgctggtggt gctcttttcc
    10321 gcaggtgcca gctattacat gaccttcctt aatgtggtca ttggttatgg catcatcgct
    10381 tccgtcatga ccaccgatat cgacctgtca aaagaagttg ttggtctgaa ctttattctc
    10441 tggttaatcg ccgttagtgc attgcctctt atccttatct ggaataaccg ctgtcgctac
    10501 accttgctcc gacaactgcg aaccccaggg cagcgtattc gcagcctggt ggtcgtcgta
    10561 ctggcgggta ttatggtttg ggcaccgatt cgtttgctgg atatccagca gaagaaagtg
    10621 gagagggcga ccggcgttga tttgccgagt tatggcggtg tcgtagcgaa ctcttatctg
    10681 ccatcaaact ggctttctgc gttggggctg tatgcctggg cgcgggtcga tgaatcttcc
    10741 gataataatt cattgcttaa tccggcgaag aagtttacat accaggctcc acaaaacgtt
    10801 gatgatactt atgtcgtgtt tatcatcggt gaaaccacgc gttgggacca tatgggtatt
    10861 ttcggctatg agcgtaatac cacgccgaaa ctggcccagg agaaaaatct ggcggcgttc
    10921 cgtggttact cctgtgatac cgcaaccaaa ctctcactgc gttgcatgtt tgtacgtcag
    10981 gggggcgcgg aagataatcc gcagcgcaca ttaaaagaac agaacatttt cgcggttctg
    11041 aagcagttag gattcagttc tgacctctac gctatgcaga gcgaaatgtg gttctacagc
    11101 aacacgatgg cagacaacat tgcttatcgt gagcagattg gtgcggagcc acgtaatcgt
    11161 ggcaagccgg tagatgatat gttgctggta gacgaaatgc agcaatcgct agggcgcaac
    11221 ccggatggta agcatctgat cattctgcat accaaaggtt cgcattttaa ctacacccag
    11281 cgttatccgc gtagcttcgc gcagtggaag ccggaatgta ttggtgttga tagcggctgt
    11341 accaaagcgc agatgatcaa ctcctatgac aactcggtga cctatgtgga tcactttatc
    11401 tccagcgtga ttgatcaggt tcgcgataag aaagcgattg tgttctacgc agctgaccac
    11461 ggtgagtcaa ttaatgaacg cgagcacctg cacggcacgc cgcgtgaact ggcaccgccg
    11521 gagcagttcc gcgtaccgat gatggtctgg atgtcagata aatatctgga aaatccggcc
    11581 aatgcgcagg cgtttgcgca gctgaaaaaa gaagccgaca tgaaagtgcc acgccgtcac
    11641 gtagagctgt acgacaccat catgggttgt cttggctata cttcaccgga tggtggaatt
    11701 aacgaaaaca acaactggtg tcacatcccg cagacaaaag tggcagcggc taactaagcg
    11761 gcctgctgac tttctcgccg atcaaaaggc attttgctat taagggattg acgagggcgt
    11821 atctgcgcag taagatgcgc cccgcattcg gtgattggcg cagcctggta gcgcacttcg
    11881 ttcgggacga aggggtcgga ggttcgaatc ctctatcacc gaccaaattc gaaaagcctg
    11941 ctcaacgagc aggttttttt gcatttgtag ctcatgagga tgagaacctc cgggggcagg
    12001 gaggctcgac tcgacgtagg cctgataaga cgcgccagcg tcgcatcagg caacggcacc
    12061 gcacttattg tcggatgcgg cgcgaccgcc ttattcgacc aacacaccac acattttcct
    12121 gccaactttg cgccccatat ctcactatcc attaacggat ttgtgacaga aaccattgtc
    12181 tttttttata tttgcttaaa tcttttttgc gttacttatg gctggcgctg gcattttccg
    12241 ctgtgcgttt tagcgttcat ggtattaatc gctcagatat ccaccttttc ctcagatttt
    12301 tttcattaaa tgcataaaac ttaatcacca aatgttgcta aatattaagc tgattgttct
    12361 ggttatgttg gagtagcaca aatttctctg ctgcaaatag ctgtttgaag tttttttaat
    12421 ctttgtttgt ggattctcac cgttggtgta aatcccggtt ggctgtattg acgttttcac
    12481 attctgttga cagattgtag gtcacgaggg gcattttatg gaggatccgc actgttacac
    12541 tgatgttaat tagtacggca tccccacctc ataacgttga cccgaccggg caaaaaacaa
    12601 aaaaggtcag gcagcgacaa cccactgcaa agggttaaaa caacaaacat cacaattgga
    12661 gcagaataat gcgtatttcc ttgaaaaagt cagggatgct gaagcttggt ctcagcctgg
    12721 tggctatgac cgtcgcagca agtgttcagg ctaaaactct ggtttattgc tcagaaggat
    12781 ctccggaagg gtttaacccg cagctgttta cctccggcac cacctatgac gcctcttccg
    12841 tcccgcttta taaccgcctg gttgaattta aaatcggcac caccgaagtg atcccgggcc
    12901 tcgctgaaaa gtgggaagtc agcgaagacg gtaaaaccta taccttccat ctgcgtaaag
    12961 gtgtgaagtg gcacgacaat aaagaattca aaccgacgcg cgaaatgaac gccgatgacg
    13021 tggtgttctc gttcgatcgt cagaaaaacg cgcaaaaccc gtaccataaa gtttctggcg
    13081 gcagctacga atacttcgaa ggtatgggct tgccggagct gatcagcgaa gtgaaaaagg
    13141 tggacgataa caccgttcag tttgtgctga ctcgaccgga agcgccgttc ctcgctgacc
    13201 tggcaatgga cttcgcctct attctgtcaa aagaatatgc tgacgcgatg atgaaagccg
    13261 gtacaccgga aaaactggac ctcaacccaa tcggaaccgg tccgttccag ttacagcagt
    13321 accaaaaaga ttcccgtatc cgctacaaag cgtttgatgg ctactggggc accaaaccgc
    13381 agatcgatac gctggtcttc tctattaccc ctgatgcttc cgtgcgttac gcgaaattgc
    13441 agaagaatga atgccaggtg atgccgtacc cgaacccggc agacatcgct cgcatgaagc
    13501 aggataaatc catcaacctg atggaaatgc cggggctgaa cgtcggctac ctctcgtata
    13561 acgtgcagaa aaaaccgctg gatgacgtga aagttcgcca agcactaacc tacgcggtga
    13621 acaaagacgc gatcatcaaa gcggtttatc agggcgcggg tgtatcagcg aaaaacctga
    13681 tcccgccaac catgtggggc tataacgacg acgttcagga ttacacctac gatcctgaaa
    13741 aagcgaaagc cttgctgaaa gaagcgggtc tggaaaaagg tttctccatc gacctgtggg
    13801 cgatgccggt acaacgtccg tataacccga acgctcgccg catggcggag atgattcagg
    13861 cagactgggc gaaagtgggc gtgcaggcca aaatcgtcac ctacgaatgg ggtgagtacc
    13921 tcaagcgtgc gaaagatggc gaacatcaga cggtaatgat gggctggact ggcgataacg
    13981 gggacccgga taacttcttc gccaccctgt tcagctgcgc cgcctctgaa caaggttcta
    14041 actactcaaa atggtgctac aaaccgtttg aagatctgat tcaaccggcg cgtgctaccg
    14101 acgaccataa caaacgcgtt gaactgtaca agcaagcgca ggtggtgatg cacgatcagg
    14161 ctccggcact gatcatcgct cactccaccg tgtttgaacc ggtacgcaaa gaagtcaaag
    14221 gctatgtggt tgatccatta ggcaaacatc acttcgaaaa cgtctctatc gaataattaa
    14281 aagccataca agactgatgg caaaggcaaa actgcctgat gtgctccgct tatcaggcct
    14341 acgaaaattc tgcaatgtat tgaatttgca cgattttgta ggccggataa ggcgttaacg
    14401 ccgcatccgg cataaacaaa gcgcactttg tcaacaatct gtatacccgg tggcgctgtg
    14461 cctctgcgtg cagcgccatc cggcagcaat acttcattct ctgtccgaca agggcgggaa
    14521 ttgatttgtg agcaatacag acacgcagtt ccaggctgcg agtcactata gagaatccgg
    14581 gttatgttgc agtttattct ccgacgtttg ggactcgtca tccccacgtt tatcggtatt
    14641 acccttctca catttgcctt tgtccacatg atcccgggcg atccggtgat gatcatggca
    14701 ggcgaacgtg ggatctcccc tgagcgtcac gtgcagctgc tggctgaact cggcttagat
    14761 aaaccgatgt ggcagcagta tctccattac atttggggcg taatgcacgg cgatctaggc
    14821 atttcaatga aaagccgaat tccagtatgg gaagagttcg tgccgcgctt tcaggccacg
    14881 ctggaacttg gcgtctgcgc gatgattttt gctacggcag tcggtattcc ggttggtgtg
    14941 ctggctgcgg ttaaacgcgg ttccattttc gatcacactg cagttggcct ggcgctgacc
    15001 ggctactcga tgccgatctt ctggtggggc atgatgctga tcatgctggt ttcggtgcac
    15061 tggaacctga cgcccgtctc cggtcgcgtg agcgatatgg tgttcctcga tgactccaat
    15121 ccgttaaccg gttttatgct aatcgacacc gccatctggg gtgaagacgg caactttatc
    15181 gatgccgtcg cccatatgat cttgcccgcc attgtgctgg gtactattcc gctagcggtc
    15241 attgtgcgta tgacacgctc ctcgatgctg gaagtgctgg gcgaggatta catccgcacc
    15301 gcgcgcgcca aagggctaac ccgcatgcgg gtgattatcg tccatgcgct gcgtaacgcg
    15361 atgctgccgg tagtgaccgt tatcggcctc caggtgggaa cattgctggc gggggcgatt
    15421 ctgaccgaaa ccatcttctc gtggcccggt ctggggcgct ggttgattga cgcactgcaa
    15481 cgccgcgact atccggtagt gcagggcggc gtattgctgg tggcgacgat gattatcctc
    15541 gtcaacttgc tggtcgatct gctgtacggc gtggtgaacc cgcgtattcg tcataagaag
    15601 taaggggaca tcatgtcaca ggttactgaa aataaagtga ttagcgcacc ggtgccgatg
    15661 accccgttac aggagttctg gcactatttt aaacgcaaca aaggcgcggt cgtcgggctg
    15721 gtttacgtcg ccatcgtgct gttcatcgcg atctttgcca actggattgc gccttataac
    15781 ccggcggatc agttccgcga tgcgctgctc gccccgccag cctggcagga aggcggcagt
    15841 atggcacact tgctgggcac cgatgacgtc ggtcgtgatg tgctgtcgcg gctgatgtac
    15901 ggcgcgcgtt tatcgctgct ggttggctgt ctggtggtgg tgttgtcgct gattatgggc
    15961 gttattctcg gcctgatcgc cggttacttc ggcggcctgg ttgataacat tatcatgcgc
    16021 gtggtcgata tcatgctggc gctgccaagt ctgctgttgg cactggtgct ggtggcgatt
    16081 ttcggcccgt cgattggtaa cgccgcgctg gcgctgacct tcgttgcctt gccgcactat
    16141 gtgcgcttaa cccgcgccgc cgtgctggtg gaggtgaatc gcgattacgt caccgcctct
    16201 cgcgtggcag gtgccggggc gatgcgccag atgtttatta acatcttccc gaactgcctt
    16261 gcgccgctga ttgttcaggc gtcgctcggt ttctctaacg ccattctcga tatggctgct
    16321 cttggtttcc tcggcatggg ggcacagccg ccaacgcctg agtggggcac catgctctcc
    16381 gacgtgttgc agttcgcgca aagcgcctgg tgggtcgtga ccttccctgg tctggcgatc
    16441 ctgttgacgg tgctggcatt taacctgatg ggtgacggtc tgcgtgacgc gctcgatccc
    16501 aaactaaagc agtaagaggt tcgagatggc gttattaaat gtagataaat tatcggtgca
    16561 tttcggcgac gaaagcgcac cgttccgcgc cgtagaccgc atcagctaca gcgtaaaaca
    16621 gggcgaagtg gtcgggattg tgggtgagtc cggctccggt aagtcggtca gttcactggc
    16681 gattatgggg ctgattgatt atccgggccg cgtaatggca gaaaaactgg agtttaacgg
    16741 ccaggatttg cagcgtatct cagaaaaaga gcgccgcaac ctggtgggtg ccgaagtggc
    16801 gatgatcttc caggacccga tgaccagcct taacccgtgc tacaccgtgg gtttccagat
    16861 tatggaagcg attaaagtgc atcagggcgg caacaaaagt acccgccgtc agcgggcgat
    16921 tgacctgctg aatcaggtcg gtattcccga tccggcttcg cgtctggatg tttacccgca
    16981 tcagctttcc ggcggcatga gccagcgcgt gatgatcgcc atggcgattg cctgtcggcc
    17041 aaaactgctg attgccgatg aaccgaccac cgcgctggac gtgaccattc aggcgcaaat
    17101 catcgaacta ctgctggagc tacagcagaa agagaacatg gcgctggtgt taattaccca
    17161 tgacctggcg ctggtggcgg aagctgcgca taaaatcatt gtgatgtatg caggtcaggt
    17221 agtagaaacg ggcgatgcac acgccatctt ccatgcgccg cgtcacccgt atactcaggc
    17281 attgctgcgt gcgctgccag aatttgctca ggacaaagaa cgtctggcgt cgttgccagg
    17341 tgttgttccc ggcaagtatg accgcccgaa cggctgcctg cttaacccgc gctgccccta
    17401 tgccactgac agatgtcgcg ctgaagaacc ggcgctgaat atgctcgctg atgggcgtca
    17461 gtccaaatgc cattacccac ttgatgatgc cgggaggccc acactatgag tacgcaagag
    17521 gccacctcgc aacaaccgct gttgcaggct atcgacctga aaaaacatta tccggtgaag
    17581 aaaggcatgt tcgtgccgga acgtctggtg aaggcgctgg acggcgtttc gtttaacctt
    17641 gaacgtggca aaacgctggc agtagtgggc gaatctggct gcggtaaatc gaccctcggt
    17701 cggttgctga cgatgatcga aacgcccacc ggcggcgagc tgtattatca ggggcaggat
    17761 ctgctcaagc acgatccgca ggcgcagaag ctgcgtcggc agaaaatcca gatcgtcttc
    17821 cagaatccct acggttcgct gaatccgcgt aagaaagttg ggcaaattct cgaagagccg
    17881 ctgcttatta acaccagctt aagcaaagaa cagcgtcggg aaaaagccct gtcgatgatg
    17941 gcaaaagtcg gcctgaaaac cgagcactat gaccgctatc cgcacatgtt ttccggcgga
    18001 caacgtcagc gtatcgctat tgcccgtggt ctgatgctcg acccggatgt ggtgattgcc
    18061 gatgagccgg tttctgcgct ggacgtctca gtgcgtgcgc aggtgctgaa tctgatgatg
    18121 gatttgcagc aggagttggg gctttcttat gtctttatct cccacgacct gtcggttgtg
    18181 gagcacattg ctgatgaagt gatggtgatg tacctaggcc gctgcgtgga gaaaggaacg
    18241 aaggaccaaa tcttcaataa cccgcgccat ccgtacaccc aggcgctgct ctccgcgacg
    18301 ccacgcctga acccggacga tcgtcgtgaa cgcatcaaac tcaccggtga actgccaagc
    18361 ccgctgaatc ctccgccggg ttgcgccttc aacgcccgct gtcgtcggcg cttcggcccc
    18421 tgcacccagt tgcagccgca gctaaaagac tacggaggtc aactggtagc ttgttttgct
    18481 gttgatcagg atgaaaatcc gcagcgttaa ctcacaaaac cggggccatg cgctccggtt
    18541 tactcaatca gcttcaacag tggcgacaag catagcaaaa tgccgtaaag cagaatcagc
    18601 caggctttaa atccgcgaag tttttccagt tgtgcgactt tatagatcag gaaaaacgga
    18661 atgaggcacg acacaatgcc atataacggg cttcccaact gaaagaacac cagcactgat
    18721 acacgaaacg aaacccaaat cgtcaacgta atgacgataa aagcgcagat cgccagagtc
    18781 agcatgcgtg agttaatttt cttggtatca ataattcggc ttaacagatt gagaataatg
    18841 cctttaatag cctcgtggaa accgagataa atgccaaaga atgcggtcag tacggcaaag
    18901 atattcagca ccgtagaggt gatatgaatg atatgtccag ggatcacctg cgcggccagt
    18961 gccagtgccg agatattttg ttcaaaggca gaaacggctt cttcgtggct aattgagaag
    19021 gtaaacgaaa aggcaaaaaa caggatcacc gcgatgagcg tgatataact aattcggtgg
    19081 gtacgcagcg cgagccgggt tgccagtact ttatctgctt cccgtttacg ataggcaata
    19141 ttcattgggt taagtacctg aataaatacc gcagaaaaga agcaaaatgg aatggtaagc
    19201 aggacatcgc ggaaaaagac ggaggcttgc gggaaggcgg ttatattggc gaaattccag
    19261 tgcgggatca tcgcaaaacc gaacacgaca ataatcccta ctttgaccac caccattggc
    19321 ccagaaatct taaacagtaa tcgttcacca ccagacgcaa tcgctatcag tacggcgaaa
    19381 atagcgactt tataaagtag agattgtgaa agatcggcat cggttaaacc gaaggttttc
    19441 aggtacgagg cgctgtcgaa aaccacggag agagagtaga taaaaatccc gtggataatc
    19501 atcaaaaagt agataacccc gaggaaaatt ccccagttct tccccaggta atgactgata
    19561 atatcggtgt agtcattaca ggaatcgctt tcagaaaggg tttttaaata aatgtcctgc
    19621 actacccagg tgacaggata agcaatgatc gctgcggtaa taaataccca aattcccttc
    19681 aagccaattt gtactggcat cagcacggtt ccggcaccaa tcgccatgcc tatgcataat
    19741 aaaacccagc cgaagtcgta gcgtgtaaac ggcaatttct gattgtgttt cgatagtgtg
    19801 ttgtgctgca taaagtcctg tccagaaaga agtcggcctc gcaaaaatga ataaacccag
    19861 tttctcttgc gcaacaggtg gagaagtcga atacgcagta tgtaaaaaat gaggcaacag
    19921 attcactggg gaggtcgcga ggaggcgcaa ttctacaaaa ttgcgcattt cactaccagt
    19981 ctggagtcag cccggatatg aactgcaact attgcaaata aagaaattaa gctggaaggc
    20041 atcgcgcaat gaggaagatt gccgggtgct gacagtaaaa agcaatcgta gaatcagcgt
    20101 caagccggaa ggattccggt gaggcgcaat gttgcggggg cttaatccct ggtggcattg
    20161 gttgctggag agagaaaacc cccgcacgtt gcaggtatgt acctgacaac ataacggggg
    20221 ctaatcttga acctagacca ctcaagaata gccgcgaaac gttgtcatta caacacaggc
    20281 ggctatatga cgctcgcaga gctgggcatg gccttctggc atgatttagc ggctccggtc
    20341 attgctggca ttcttgccag tatgatcgtg aactggctga acaagcggaa gtagcgtgtc
    20401 atgtgggcgt caggctgccg taatggcaat ttgcgcccgg accaggccgc aggggtgaaa
    20461 ctctgcggcc tttttcgtaa gaaaaatgca acggttgcaa gcggataata ttctgcggaa
    20521 tgcgcagcac agagtgaaga tgagacggat tccggaacag aaaaacagtc gtagaatcag
    20581 cgtcaagccg gaaggattcc ggtgaggcgc aatgttgcgg gggcttaatc cctggtggca
    20641 ttggttgctg gagagagaaa acccccgcac gttgcaggta tgcacctgac aacaccacgg
    20701 gggccattct tgcatccaaa caatacaaga atagccgcga aacgttgtca ttacaataca
    20761 ggcggctata tgacgttcgc agaactgggc atggccttct ggcatgattt agcggctccg
    20821 gtcattgctg gtattcttgc cagtatgatc gtgaactggc tgaacaagcg gaagtaacgt
    20881 gccatgcggg cgtcaggctg ccgtaatggc aatttgcgcc cggaccaggc cgcaggggtg
    20941 aaactctgcg gcctttttcg taagaaaaat gcaacggttg caagcggata atattctgcg
    21001 gaatgcgcag cacagagtga agacgtgacg gattccggaa cagaaaaaca gtcgtagaat
    21061 cagcgtcaag ccggaagggt tccggtgagg cgcaatgttg cgggggcttt atccctggtg
    21121 gcattggttg ctggagagag aaaacccccg cacgttgcag gtatgcacct gacaacatca
    21181 cgggggccat tcttgaacct gaacaactca agaatagccg cgaaacgttg tcattacaac
    21241 acaggcggct atatgacgct cgcagaactg ggcatggcct tctggcatga tttagcggct
    21301 ccggtcattg ctggcattct tgccagtatg atcgtgaact ggctgaacaa gcggaagtaa
    21361 cgtgtcatgc gggcgtcagg ctgccgtaat ggcaatttgc gcccggacca ggccgcaggg
    21421 gtgaaactct gcggcctttt tcgtaagaaa aatgcaacga ttgcacgcgg ataatattct
    21481 gcggaatgcg cagcacagag tgaagacgtg acggattccg gaacagaaaa acagtcgtag
    21541 aatcagcggc aagccggaaa ggttccggtg aggcgcaatg ttgcgggggc tttatccctg
    21601 gtggcattgg ttgctggaga gagaaaaccc ccgcacgttg caggtatgca cctgacaaca
    21661 tcacgggggc taatcttgaa cccaaacaac tcaagaatag ccgcgaaacg ttggcattac
    21721 aacacaggcg gctatatgac gctcgcagaa ctgggcatgg ccttctggca tgatttagcg
    21781 gctccggtca ttgctggcat tcttgccagt atgatcgtga actggctgaa taagcggaag
    21841 taacgtgtca tgcgggcgtc aggctgccgt aatggcaatt tgcgcccgga tcaggccgca
    21901 gaggttaaac tctgcggcct ttttcgtgct tactgcgggt aaggcaccca gtcgccgccg
    21961 ttcaggcgaa cgtacggttt atcttggtat tgaataacta ctgcatttga gttctcggag
    22021 accggtgctg tttgtggcaa cccactggtg agttttttcc agtcaacatt gtcttcggtg
    22081 aaaatcttgc catcaagaac gcgaaccacc agatcggaga tagccaggaa gctgctcggt
    22141 tggtcaatga caatcggtgc cccctgatgt ggtgccttca tgccgaagaa tttcacccca
    22201 acggggacgt ctgtgataga cgggctaggg atatcacgta ggccagatac ctgcattctg
    22261 tcgcccttca gcgcgccgcc gtgttccggc accacgacca ccatcacttt acgacccgat
    22321 ttctccagtt cagtaaagaa ggcgtccagt tcatcaaaga atttctgcgc ccgcgctttg
    22381 taatccgctg ttttgctgac ccccggataa tggttgccgt catgcagtgg aagcgtgttg
    22441 tagaacgtgg cactacggct gtttttatct ttttcggtaa cgtccagcca gcggttaagc
    22501 acggcggtat cgtcataaac cggcgaacca tcaaagccca gcaaaataac cggcagattt
    22561 gtttgatcca tcaattcagt ctgcatgccg ccattttcgc gaacttcttt caaaaaaccg
    22621 ccgaactggc cgttatgtcc catcatcagg tgctgggtaa agcccagttt cgaaaggtta
    22681 tcaaacagat agcagtcgtt atttgccggt tgatacagat tagtgtgcga agtctgcccg
    22741 cagctggcgc gcagtaaacg gatcgccgcc gggccactgt aggaggtggc ggagttaaag
    22801 ttcttgaaat caatatcgaa atgcgaccac agtggatgcg acatcaaccc ggcggcttct
    22861 atatccgacc aggaaagcga acagatgtta atcaccagta gttcaaatgg ctgagcatca
    22921 gcgggcagcg aagacgggaa ggtcgattta cgtttcgcct ccgcgttata gaaattattc
    22981 agccaggcgt taaggttcgc cgttgttggc ggtgcagttt gtgcgggcat atcacccact
    23041 accggtgcgc cacccgtcgc cgcaacggtt gccgctgcgt taccacccgt cgttgttaca
    23101 gtggtcgtcg gttgtccggc tggccacaag gagaaacttg gtcccgccag ggtaagtacg
    23161 ttcagccata gcagtatggc aaccacaaaa acggtaatgc gaatccattg tgacaggaat
    23221 aaccaggcca ctaataaaac aaaaatggcc ccaatcatct gccagttaat aaagcgtgtg
    23281 acaaggtcga ttaaataatc ggtactgaac cccgccacct gcgaaccctg gctcattatg
    23341 ctttccgggc caggcaacca gatgtcatgc cagaacaaag caaagccgat cggcagggca
    23401 atccagtggc gcaagcgatg caggctgtaa cgcggaatgg gcatcagcag aaacgcggca
    23461 aacaccaaat tgaggagcgg gtggaagtta agatatcccg cccacaacag gccaaactta
    23521 accagaaaat agaagttcca gccggaaagg ccgcgccagt attgccagag ggaagaaggc
    23581 atggcggtat tttgcgtaaa ttgagtcatt ttttggttgc cctggctttt tccgtgcggc
    23641 gtaacgtccc ggccggttta acataacgag gtttagcaaa gaacaaacgt aaggtgtcgc
    23701 gaatgcgtcg caaagagtgc cgcgccagat agcccagcgg gaaaaatatc agtgcgcaaa
    23761 cgataataat ttcaatgata tcgctgatgg tcatcatgat gagcgctcca cagcatcatc
    23821 taacagtcgc atgggttctg gtattcgtcg ccagtggcga ccatcgtgct cggcattgat
    23881 gaccggttta gaactttgcg ttaaaggcag cggcattccc cattgttctg gggcaagcag
    23941 gcgcatctgc accagctcgg cactgatttg atcatcttca aaccagacca tacggtttga
    24001 gaaaatgtcg ccagtaggca atgggaaaat atgattcaac gcggtatcca gatcgttaat
    24061 ccgacagaat gagagaaaca gcaccagccg attaccgcca atggtcatga tatcgccggt
    24121 acggttaggg cgacatagcg tcagggcttg ctcaacgcgg atacccggta ccggacgtag
    24181 ggcaaccaga acgcctttac cgtgggcagg caagagaggg ttattcatca tgttgttgac
    24241 ggcattacag aacacatccc acttctggaa accacgcagt ttgagcggct gggtcattga
    24301 cagcaaggta gtgatatctt ccggtacata gcgactaaac ttctgccctt gcacgctttc
    24361 gatcatcgtc agacaacggg agagtggcgc attccacgga ataaccatat ttgcaccgca
    24421 ggccaataac aaacgttcat cggtggcgcg caggctagcg gtattttccc gcacgaggat
    24481 tttcatcgca ctaccgcgct ggcgacgcag ggtatgaatg ctgcgggcca gtggctcgat
    24541 ttgcgcattt tgctgtaaag aaaagaccac cgtcgccgcc tgagcggtac gggcttcatt
    24601 gaacaggact tcgttattgt tgaacagttg ccagtgttcc gatagcggcg gcgcaccttc
    24661 cagtacagca acattactca gaatgcgttt ttcgtcgctg cgtggttgga tctccgcctc
    24721 ttcgctttga actaatgtcc agataccatt ttgttgctga acgctaagct gctgacgggc
    24781 gctgacccct ttttcgttgc accagaaggc aatatccagc aaatgttggt caccctgaaa
    24841 acgcaaactg gcaagaccaa aaagtgaacg gtattcctca agcaacaatg aaaattgttt
    24901 atcgttatta tttccgggat taattaccaa aagcgaacaa tggtttaacc tgctccattt
    24961 attcatttta tccaaccatg agcgaagccg ctcggcagga atattttgcc atgcgttatt
    25021 tgcgcaaaca agaataaaaa ggtaattatg gggatcaata gaacactgca aatcacgggc
    25081 caaatagtat agaccttttt catgatttag cattgaaaat aattttattt tttccggccc
    25141 ttgagaatca tctaattgaa agattttcgc cggatcgctg tccatgctaa tgaccgcgac
    25201 gtgtgcggtt tcagcctggg atgcaattgt ttgattcgcc agactgatag catcttcatg
    25261 gcgatcgacg ttaaaccacc agacgccgcc tgctggcata tgtcgcagct catcccataa
    25321 tgatgagata ccgatagaga atacagggtc cacaatgtcc ctcatgtcac cattttgtct
    25381 gattatcagt ttactagcga aagctaagaa ataaacctac cattgaaatt aaaacttatc
    25441 agatttagca tgtgaatcaa tttagaaggt cacaagacgt gtgattttag tcttttcgtt
    25501 tttaactttt tctgttgcgt aacggaaagt caaaaagtga gcaaattccc gtctcgccgt
    25561 aaacaataaa ccggcgaaat gctcacggtt agaattgtct catgaatggt acggttattt
    25621 catagggatc aagcaaaatg aataacaatg aaccagatac tctgcctgat cccgcgatag
    25681 gctatatctt ccagaatgat attgtggcgt taaagcaggc attttcactg cctgatattg
    25741 attatgccga tatttcccaa cgcgaacagt tggccgcggc attaaaacgc tggccgttgc
    25801 tggcagagtt tgcgcaacaa aagtagggga ttggtgaatg gccgtactgg gattgcaggg
    25861 ggtgcgggga ggcgtgggga caacaaccat caccgccgca ttagcctggt cattacaaat
    25921 gttgggagaa aatgtcctgg tggtcgatgc ctgcccggac aacttgttgc gcctgtcatt
    25981 taatgttgat tttacccacc gtcagggctg ggccagagcg atgctggatg gccaggactg
    26041 gcgtgacgct gggttgcgct acacctcgca gctcgatttg ctgccttttg gtcagttatc
    26101 cattgaagaa caagaaaatc cacagcactg gcaaacccgg ctgagcgata tttgctccgg
    26161 cttacagcaa ctaaaagcca gcgggcgtta ccagtggatt ttaatcgact taccgcgtga
    26221 tgcctcgcag ataacccacc agctgctgag tttgtgcgag cactcgctgg caatcgtcaa
    26281 tgtggatgcc aactgccata tccgactgca tcagcaagcg ctgccggatg gcgcacatat
    26341 tttgattaat gacttccgta ttggcagtca ggttcaggac gatatttacc agctttggtt
    26401 gcaaagccag cgccgattac tgccgatgct cattcatcgt gatgaagcga tggctgaatg
    26461 cctggcggct aagcaaccag taggtgaata tcgcagtgat gcgctggcgg ctgaagagat
    26521 actgacgctg gcgaactggt gcctgttgaa ctactccggg ctgaaaacgc cagtcgggag
    26581 tgcatcatga gtatcctgac ccggtggttg cttatcccgc cggtcaacgc gcggcttatc
    26641 gggcgttatc gcgattatcg tcgtcacggt gcgtcggctt tcagcgcgac gctcggctgt
    26701 ttctggatga tcctggcctg gatttttatt ccgctggagc acccgcgctg gcagcgtatt
    26761 cgcgcagaac ataaaaacct gtatacgcat atcaacgcct cgcgtccgcg tccgctggac
    26821 ccggtccgtt atctcattca aacatgctgg ttattgatcg gtgcatcgcg caaagaaacg
    26881 ccgaaaccgc gcagcgggca ttttcaggtc tgcaaaatat tcgtggacgt taccatcaat
    26941 ggatgaacga gctgcctgag cgcgttagcc ataaaacaca gcatctggat gaaaaaaaag
    27001 agctcggtca tttgagtgcc ggggcgcggc ggttgatcct cggtatcatc gtcaccttct
    27061 cgctgattct ggcgttaatc tgcgttactc agccgtttaa cccgctggcg cagtttatct
    27121 tcctgatgct gctgtggggg gtagcgctga tcgtacggcg gatgccgggg cgcttctcgg
    27181 cgctaatgtt gattgtgctg tcgctgaccg tttcttgccg ttatatctgg tggcgttaca
    27241 cctctacgct gaactgggac gatccggtca gcctggtgtg cgggcttatt ctgctcttcg
    27301 ctgaaacgta cgcgtggatt gtgctggtgc tcggctactt ccaggtagta tggccgctga
    27361 atcgtcagcc ggtgccattg ccgaaggata tgtcgctgtg gccgtcagtg gatatctttg
    27421 tcccgactta caacgaagat ctcaatgtgg tgaaaaatac catttacgcc tcgctgggta
    27481 tcgactggcc gaaagacaaa ctgaacatct ggatcctcga tgacggcggc agggaagagt
    27541 ttcgccagtt tgcgcaaaac gtgggggtga aatatatcgc ccgcaccact catgaacatg
    27601 cgaaagcggg caacatcaac aatgcgctga aatatgccaa aggcgagttc gtgtcgattt
    27661 tcgactgcga ccacgtacca acgcgatcgt tcctgcaaat gaccatgggc tggttcctga
    27721 aagagaaaca gctggcgatg atgcagactc cacaccactt cttctcgccg gacccgtttg
    27781 aacgcaacct gggacgtttt cgtaaaacgc cgaacgaagg cacgctgttc tatggtctgg
    27841 tgcaggatgg caacgatatg tgggacgcca ctttcttctg cggttcctgt gcggtgattc
    27901 gtcgtaagcc gctggatgaa attggcggca ttgctgtcga aaccgtgact gaagatgcgc
    27961 atacttctct gcgtttgcac cgtcgtggct atacctccgc gtatatgcgt attccgcagg
    28021 cggcggggct ggcgaccgaa agtctgtcgg cgcatatcgg tcagcgtatt cgctgggcgc
    28081 gcgggatggt ataaatcttc cgtctcgata acccgctcac cggtaaagga ctgaagtttg
    28141 ctcagcggct gtgctacgtc aacgccatgt tccacttctt gtcgggcatt ccacggctga
    28201 tcttcctgac tgcgccgctg gcgttcctgc tgcttcatgc ctacatcatc tatgcgccag
    28261 cgttgatgat cgccctattc gtgctgccgc atatgatcca tgccagcctg accaactcga
    28321 agatccaggg caaatatcgc cactctttct ggagtgaaat ctacgaaacg gtgctggcgt
    28381 ggtatatcgc accaccgacg ctggtggcgc tgattaaccc gcacaaaggc aaatttaacg
    28441 tcaccgccaa aggtggactg gtggaagaag agtacgtcga ctgggtgatc tcgcggccct
    28501 acatcttcct tgttctactc aacctggtgg gcgttgcggt aggcatctgg cgctacttct
    28561 atggcccgcc aaccgagatg ctcaccgtgg tcgtcagtat ggtgtgggtg ttctacaacc
    28621 tgattgttct tggcggcgca gttgcggtat cggtagaaag caaacaggta cgccgatcga
    28681 accgcgtgga gatgacgatg cccgcggcaa ttgcccgcga agatggtcac ctcttctcgt
    28741 gtaccgttca ggatttctcc gacggtggtt tggggatcaa gatcaacggt caggcgcaga
    28801 ttctggaagg gcagaaagtg aatctgttgc ttaaacgcgg tcagcaggaa tacgtcttcc
    28861 cgacccaggt ggcgcgcgtg atgggtaatg aagttgggct gaaattaatg ccgctcacca
    28921 cccagcaaca tatcgatttt gtgcagtgta cgtttgcccg tgcggataca tgggcgctct
    28981 ggcaggacag ctacccggaa gataagccgc tggaaagtct gctggatatt ctgaagctcg
    29041 gcttccgtgg ctaccgccat ctggcggagt ttgcgccttc ttcggtgaag ggcatattcc
    29101 gtgtgctgac ttctctggtt tcctgggttg tatcgtttat tccgcgccgc ccggagcgga
    29161 gcgaaacggc acaaccatcg gatcaggctt tggctcaaca atgatgataa cgcgatgaaa
    29221 agaaaactat tctggatttg tgcagtggct atggggatga gtgcgttccc ctctttcatg
    29281 acgcaggcga cgccagcaac gcaaccactg atcaatgctg agccagctgt agccgcccag
    29341 acggaacaaa atccgcaggt ggggcaagtg atgtcaggcg agcagggagc tgatgccccc
    29401 atcgtggcgc agaacggtcc ttcgcgtgat gtgaagctga cctttgcgca aatcgcgccg
    29461 ccgccgggca gcatggtgct acgtggtatt aacccgaacg gcagcattga gtttggtatg
    29521 cgcagcgatg aagtggtgac gaaggcgatg ctcaacttcg aatacacgcc atcgccatcg
    29581 ttactgcctg tccagtcgca gttaaaggtt tatctcaatg atgagctgat gggcgtgctg
    29641 ccagtgacca aagaacagtt gggtaaaaaa acgctggcgc aaatgcccat taatccactg
    29701 tttattaccg acttcaaccg tgtgcggctg gagtttgtcg gccattatca ggacgtgtgc
    29761 gaaaacccgg ccagcaccac gctttggctg gatgttgggc ggagcagtgg actggatctg
    29821 acctatcaga ccctgaatgt gaagaatgac ctgtcacact tcccggtgcc attctttgac
    29881 ccgcgcgata accgtactaa caccttgccg atggtctttg cgggtacgcc ggatgttggg
    29941 ctgcaacaag catctgccat tgtcgcctcg tggtttggtt cgcgttctgg ctggcgtggg
    30001 cagaacttcc cggtgctcta taaccaattg ccggatcgca atgccattgt ctttgcaacc
    30061 aacgacaaac ggccggactt cctgcgcgat catccggcgg taaaagcccc ggtgattgag
    30121 atgattaacc atccgcagaa tccttacgtc aaactgctgg tggtgtttgg tcgtgacgac
    30181 aaagacctgt tgcaggcagc gaaaggtatc gctcagggta acattctgtt ccgtggtgaa
    30241 agcgtggtag tgaatgaagt gaaaccgctg ctaccgcgta agccgtacga tgcgccgaac
    30301 tgggtacgta ccgatcgtcc ggtcacattt ggcgaactga aaacctatga agaacagtta
    30361 caatccagcg gccttgagcc agcagcgatt aacgtttcgc taaacctgcc gccggatctc
    30421 tacctgatgc gcagtaccgg cattgatatg gatattaatt accgctacac catgccgccg
    30481 gtgaaagaca gttcgcggat ggatatcagc ctgaataacc agttcctgca atccttcaac
    30541 ctgagcagca aacaggaggc gaaccgtctg ctgctgcgta ttccggtatt acaaggtttg
    30601 ctggatggca aaacagatgt ctctattccg gcgctgaaac tgggcgcgac caaccagctg
    30661 cgcttcgact ttgagtatat gaacccgatg ccaggcggtt cggtagataa ctgtattacc
    30721 ttccagccgg tgcagaatca tgtggtgatt ggtgacgatt ccaccatcga cttctcgaag
    30781 tattaccact tcatcccgat gccggatcta cgcgcctttg ctaacgcggg cttcccattc
    30841 agccggatgg cggatctgtc gcaaaccatc accgtgatgc cgaaagcgcc taacgaagca
    30901 cagatggaaa cgttgctgaa tactgttggt tttatcggcg cacagacggg cttcccggcg
    30961 attaatctga cggtgaccga tgatggcagc accattcagg gcaaagatgc cgacatcatg
    31021 atcatcggtg gtatcccgga caaactgaaa gacgataagc agatcgacct attggtgcag
    31081 gcgaccgaaa gctgggtgaa aacaccgatg cgccagaccc cgttccccgg cattgtgccg
    31141 gacgagagcg atcgcgcggc agaaacccag tcaacgctga cctcttccgg tgcgatggcg
    31201 gcggtgattg gcttccagtc gccgtataac gaccagcgca gcgtgattgc gctgttggca
    31261 gatagcccac gcggttatga aatgcttaac gatgcggtga acgatagcgg caaacgcgcc
    31321 accatgttcg gttcggtcgc ggtgatccgc gagtccggta tcaacagcct acgtgttggc
    31381 gacgtttatt acgtaggtca tctgccgtgg ttcgagcgct tgtggtatgc gctggcaaac
    31441 catccgattc tgctggcggt gctggcgact atcagtgtga tattgctggc atgggtactg
    31501 tggcgtctgc tgcgaattat tagtcgtcgt cgtcttaacc cggataacga gtaattgaag
    31561 atgaatgtgt tgcgtagtgg aatcgtgacg atgctgttgt tggctgcctt tagtgttcag
    31621 gcagcctgta cctggcctgc ctgggagcag tttaaaaagg attacatcag tcaggaaggg
    31681 cgcgtcatcg accccagcga cgcgcgcaaa atcaccacct ccgaagggca aagttacggt
    31741 atgttctttg ccctggcggc taacgaccgt gcagctttcg ataatattct cgactggacg
    31801 cagaacaatc tcgctcaggg ttctttaaaa gaacgtttgc ccgcctggct gtggggcaag
    31861 aaagagaaca gtaagtggga agtgctggac agcaattcgg cctccgatgg tgatgtctgg
    31921 atggcctggt cgttgctgga ggcggggcgt ttgtggaaag agcagcgtta taccgacatc
    31981 ggcagcgcgt tgctaaaacg tatcgcgcgg gaggaagtgg tgacggtgcc tgggctgggt
    32041 tccatgttgt taccgggcaa agtgggtttt gctgaggata acagctggcg ttttaacccc
    32101 agctacatgc cgccgacgct ggcgcagtat ttcacccgct ttggcgcgcc gtggaccacg
    32161 ctgcgcgaaa ccaatcaaca tttattgctg gaaaccgccc cgaaaggctt ttcgccagac
    32221 tgggtgcgct atgagaaaga caaaggctgg cagctaaaag ccgaaaaaac attgatcagc
    32281 agctacgacg ctatccgcgt ttacatgtgg gtaggcatga tgcctgacag cgatccgcaa
    32341 aaagcgcgga tgctcaaccg gtttaaaccg atggcgacat tcactgagaa aaacggttat
    32401 ccgccggaaa aagtggatgt ggctacgggg aaagcgcagg gtaaaggacc ggtcggtttt
    32461 tctgccgcca tgctgccttt tttacaaaac cgcgatgcgc aggcagttca gcgccagcgc
    32521 gtggccgata actttcccgg cagcgatacc tattacaact atgtgctgac cctgtttgga
    32581 caaggctggg atcaacaccg tttccgcttc tcgacaaaag gtgagttatt acctgactgg
    32641 ggccaggaat gcgcaaattc acactaaaca tattcacgct ttccctcggt ctggccgtca
    32701 tgccgatggt cgaggcagca ccaaccatca gcagaccctg cggatggaca gcggcaacac
    32761 taatgccgtg cgcgggctgg cgaatattta ccgccagcag tcgccagaaa aagctgaagc
    32821 gtttatcgcc tcgctctctg ccagccagcg gcgcagcatt gatgatatcg aacgcagcct
    32881 gcaaaacgac cgtctggcac agcaggcaga ggtactggaa aaccagggca aatgggcgca
    32941 ggcggcagca cttcagcggc aacgactggc gctgggcccc ggcagcgtat ggattactta
    33001 ccgactggca ggaagaagct gcgcggcaat ccaacgatgg cggcagcagt cagggcttcg
    33061 gctacacggc gcgggcatta cttgaacgac gtgttacttc caactggttt gttggcacgg
    33121 caattgatat ccagcaggtg aaagattacg cacccagcca tttcctgctc tacgtacgtt
    33181 attccgccgc cggatggcag ggtgacatgg atttaccgcc gcagccgctg ataccttacg
    33241 ccgactggta agttttcaga tagcgcctct cttaatgccg ctgcgatcgg gtatactcgg
    33301 gcggcaatct gggatttccg gggggagaca atttgcgcgt aagtcgctcg ttaacaatca
    33361 agcagatggc aatggtggca gccgttgtcc tggtgttcgt ttttattttt tgcaccgttt
    33421 tgctgttcca tctggtccag cagaatcgct ataacacggc tacgcaactg gaaagcattg
    33481 ctcgctctgt ccgcgaaccc ttatcttcag ctattttgaa aggcgatatt cccgaagcgg
    33541 aagctattct tgccagcatt aaaccggcag gcgtggtcag ccgtgccgat gtagtgctgc
    33601 ctaaccagtt ccaggcgctg cgtaaaagtt ttattccaga gcgtccggtg ccggtaatgg
    33661 ttactcgcct gtttgagcta ccggttcaaa tctcgctggg cgtctactcg ctcgaacgtc
    33721 cggcaaatcc gcagccaata gcctatctgg tgctacaggc ggattccttc cgtatgtata
    33781 agttcgtgat gagcactctc tcaacgttag tgaccattta cttacttttg tcgcttatcc
    33841 tgaccgtcgc catcagctgg tgcattaacc gcctgatttt gcatccgtta cgcaatattg
    33901 ctcgcgaact taacgccatc ccagcccagg agcttgttgg tcaccaactg gcattaccgc
    33961 gtctgcatca ggacgatgaa atcggtatgt tggtgcgcag ttacaacctc aaccagcaat
    34021 tgctgcagcg ccattatgaa gaacagaacg aaaatgcgat gcgcttcccg gtgtcggatt
    34081 tgccgaacaa agccttgctg atggagatgc tggagcaggt tgtcgcgcgt aaacaaacca
    34141 ccgcgctgat gatcatcacc tgtgaaaccc tgcgtgatac tgcgggcgtg ctgaaagagg
    34201 cgcaacgaga aattctgctg ctgacgctgg tggaaaaact caaatcggta ctgtcgccac
    34261 gtatgatcct cgcgcagatt agcggttatg actttgctgt cattgccaac ggtgtacagg
    34321 aaccgtggca cgcaatcact ttgggtcagc aagtactcac tatcatgagc gagcgcctgc
    34381 cgattgaacg tattcaactc cgtccgcact gtagcattgg cgtggcgatg ttctacggcg
    34441 atctcaccgc cgaacagctt tacagtcgcg ctatttctgc ggcatttacc gctcgccata
    34501 aaggcaagaa tcagattcag ttctttgatc cgcagcagat ggaagcagcc cagaagcggt
    34561 tgacggaaga gagcgatatc cttaatgcac tggaaaatca tcagtttgca atttggttac
    34621 agccacaggt cgagatgacc agcggtaaac tggtcagtgc ggaagtgtta ctgcgtatcc
    34681 agcaaccgga tggcagttgg gacctgccgg atggcttaat cgatcgcatt gagtgctgtg
    34741 ggctgatggt taccgtcggt cactgggtgc tggaagagtc ctgtcgattg cttgcagcct
    34801 ggcaagagcg cggcattatg ctgcccttgt cggtaaacct ctctgcgctg caactgatgc
    34861 acccgaatat ggtggcggat atgctggaac tgttaacccg ctatcgcatt cagccgggaa
    34921 cactgattct ggaagtgaca gaaagccgac gtattgacga ccctcatgct gcggtggcaa
    34981 tcctccgtcc gctgcgcaat gccggagttc gggtggcgct ggatgatttc ggcatgggct
    35041 acgcagggct gcgtcagctg cagcatatga aatcgttgcc aatcgacgta ctgaaaatcg
    35101 acaaaatgtt tgttgaaggc ttgccggaag atagcagcat gattgctgca attatcatgc
    35161 tggcgcagag cctgaactta caaatgattg ccgaaggcgt ggagactgaa gcacaacgag
    35221 actggctggc aaaagcgggc gttggtattg cccagggctt cctttttgct cgcccactcc
    35281 ctattgaaat cttcgaagag agttacctgg aagaaaagta gctaccccaa actgattaca
    35341 aaactttaaa aagtgctggt ttgtgcgagc cagctcaaac tttttaacct ttttgtttca
    35401 attatgatcc aggtacattt ctgtgatgtt gtctgggtgt tattttaagg ccgcaggtac
    35461 ccccccccca taaccttaca agacctgtgg ttttactaaa ggacacccta tgaaaacctc
    35521 tctgtttaaa agcctttact ttcaggtcct gacagcgata gccattggta ttctccttgg
    35581 ccatttctat cctgaaatag gcgagcaaat gaaaccgctt ggcgacggct tcgttaagct
    35641 cattaagatg atcatcgctc ctgtcatctt ttgtaccgtc gtaacgggca ttgcgggcat
    35701 ggaaagcatg aaggcggtcg gtcgtaccgg cgcagtcgca ctgctttact ttgaaattgt
    35761 cagtaccatc gcgctgatta ttggtcttat catcgttaac gtcgtgcagc ctggtgccgg
    35821 aatgaacgtc gatccggcaa cgcttgatgc gaaagcggta gcggtttacg ccgatcaggc
    35881 gaaagaccag ggcattgtcg ccttcattat ggatgtcatc ccggcgagcg tcattggcgc
    35941 atttgccagc ggtaacattc tgcaggtgct gctgtttgcc gtactgtttg gttttgcgct
    36001 ccaccgtctg ggcagcaaag gccaactgat ttttaacgtg atcgaaagtt tctcgcaggt
    36061 catcttcggc atcatcaata tgatcatgcg tctggcacct attggtgcgc tcggggcaat
    36121 ggcgtttacc atcggtaaat acggcgtcgg cacactggtg caactggggc agctgattat
    36181 ctgtttctac attacctgta tcctgtttgt ggtgctggta ttgggttcaa tcgctaaagc
    36241 gactggtttc agtatcttca aatttatccg ctacatccgt gaagaactgc tgattgtact
    36301 ggggacttca tcttccgagt cggcgctgcc gcgtatgctc gacaagatgg agaaactcgg
    36361 ctgccgtaaa tcggtggtgg ggctggtcat cccgacaggc tactcgttta accttgatgg
    36421 cacatcgata tacctgacaa tggcggcggt gtttatcgcc caggccacta acagtcagat
    36481 ggatatcgtc caccaaatca cgctgttaat cgtgttgctg ctttcttcta aaggggcggc
    36541 aggggtaacg ggtagtggct ttatcgtgct ggcggcgacg ctctctgcgg tgggccattt
    36601 gccggtagcg ggtctggcgc tgatcctcgg tatcgaccgc tttatgtcag aagctcgtgc
    36661 gctaactaac ctggtcggta acggcgtagc gaccattgtc gttgctaagt gggtgaaaga
    36721 actggaccac aaaaaactgg acgatgtgct gaataatcgt gcgccggatg gcaaaacgca
    36781 cgaattatcc tcttaatctc accacttatg cccgcactcc cttccccgag tgcgggtgtt
    36841 tgcgcataat tgacccctgt gcctgaactt accacctggg tgagttcagg tttatttcac
    36901 ttcttccgtg acattttcat gttcttgcgg tctaacacga agtgttttta acgtcattta
    36961 gactgaccga caacgggcag tttttttatt accaggatag ttgatcaggg gttcacatgc
    37021 agggcataaa aattcgactt ttagcgggcg gtttgctgat gatggccact gctggctatg
    37081 tgcaggcaga tgcgctccag cctgatccag catggcaaca ggggacgctt tccaacggtt
    37141 tacagtggca agtgctgacc accccccagc gtcccagcga tcgtgttgaa attcgcctgc
    37201 tggttaatac cggttcgctc gccgaaagta cacaacagag cggttacagt cacgccatcc
    37261 ctcgtattgc gctaacgcaa agcggtggcc ttgacgcagc acaggcgcgt tcattgtggc
    37321 agcaggggat cgaccctaaa cgcccgatgc cgccggtaat tgtctcttat gacaccacgc
    37381 tgtttaatct gagtttgccc aataaccgta acgatttgct gaaagaagcg ctctcttatc
    37441 tggcaaatgc cactggcaaa ttgaccatca caccagaaac catcaaccac gcgctgcaaa
    37501 gtcaggacat ggtggcaacc tggcctgccg atactaaaga gggctggtgg cgctatcgtc
    37561 tgaaagggtc aaccttgtta ggtcacgatc ctgccgatcc gctgaaacaa cccgttgaag
    37621 cggaaaaaat taaagatttc tatcagaaat ggtacacccc ggatgcaatg acgctactgg
    37681 tggtgggaaa cgtggatgcg cgctcggttg tcgaccaaat caacaaaacg tttggcgaac
    37741 tgaaaggcaa acgtgaaacg ccagctccgg tgccgacgct ttctccgctg cgtgcggaag
    37801 cggtgagtat tatgactgac gcggtgcgtc aggaccggtt atctatcatg tgggatacgc
    37861 cgtggcagcc gattcgtgaa tcagccgcac tgctgcgcta ctggcgtgcg gacctggccc
    37921 gcgaggcgct gttctggcat gttcagcaag cgttaagcgc cagtgacagc aaagacatcg
    37981 gtcttggatt tgactgccgt gtgctgtatc tgcgtgcgca gtgtgccatc aacatcgaat
    38041 caccaaacga caagctgaac agcaacctta atctggtggc gcgtgaactg gcgaaggttc
    38101 gcgataaagg tctgccggaa gaagagttca atgcgttagt ggcgcaaaag aaactggagc
    38161 tgcagaaact gtttgccgcc tatgcacggg ctgataccga tattctgatg ggccagcgga
    38221 tgcgttcgtt gcaaaatcag gtcgtcgata tcgcgccgga gcagtatcag aaactgcggc
    38281 aggatttcct taacagcctg actgtggaga tgttaaatca ggatctgcgt cagcagttgt
    38341 cgaatgatat ggcgttaata ctgctgcagc cgaaaggcga gccggaattt aacatgaaag
    38401 cgttgcaggc ggcctgggat caaatcatgg ccccatctac cgccgctgcg accacctctg
    38461 tcgccacgga tgacgtacat cctgaagtga cggatattcc acctgcacag taatgcctga
    38521 tggtgatgag ttagcacaac catcaggtat ttatggatga gctggataga ggggtaacac
    38581 tttatccagc cttttgcata tgctgcgttt acgctggcat cgcctcacgc gggataatcg
    38641 cgccgcgata ctgaataacg gtacttgcgg tcaggtgccc gcgtttcgcc gcgttttccg
    38701 cactgccgcc cgtcaaacgt accgccagat aaccggcgct gaaagagtcg ccagctgcgg
    38761 tggtatcgat cactttttct ttcggcagtt tcaccgccgg aacatcaact aacgcttcac
    38821 cagcaatgga caccaggcaa gaatccgccc cgcgtttcac caccacttct ttcacgcccg
    38881 cgttatgggt gcgcgcaatg acgtcttcca ccggctgttg accccacagc gcgtcttcgt
    38941 cgtccagcgt caggaaggcg atatccgtgc attccagcat ttgctggtac acctgctgtg
    39001 tctcttcttt gctggcccac aggcgcggac gatagttatt gtcgaaaatc acttttccgc
    39061 ctttggcgcg gcattcgcgc agcagggaaa gcagcttttc gcggctggtc gggcttaaga
    39121 tcgccaggct aatcccgctc aggtagagat aatcgaaatt cgccagctct tcgcaaatcg
    39181 ccgcagactg ctcgctcgcc agccagaatt tggcggcggc ttcgttacgc cagtagtaga
    39241 acgtacgttc gccggtgctg tcggtttcaa tgtagtaaag gcccggaaga cggttttcca
    39301 tccgttgggt cagggaagta tcaacgttct cgccgtgcca ggcgtccagc atctgctggc
    39361 taaaactgtc cgttcccagc gccgttacgt aatgaacggt taatgccgca ggatcgacct
    39421 gacgggcgat atagacggaa gtgttcaggg tatcgccgcc gaaaccgcgc ttaacgtccg
    39481 cgcctttctc ggaaagctca atcatgcatt cgccaatcac ggcaatcttt ttggacatag
    39541 tcgtgaacct gatctgtaaa aaattagcgt tagtttgcgc tgtggtggtt tgctggtcaa
    39601 ccatattaaa acagtgttcc attatttttt tgagccagaa cgtattcctg aaagattccg
    39661 ttgtggagtg aggaaattta ccccggccag gcggtaaagt tctgccctta cgcgccgata
    39721 atctttgtcg agtccgggca gcatcacttt taaacacagg acatctttga tgataaggca
    39781 ggttatccag cgaataagca accctgaagc aagcatcgag agcttgcagg aacggcgttt
    39841 ttggttgcag tgtgagcgtg cttacacctg gcagccgatc tatcaaacat gcgggcggtt
    39901 aatggccgtg gagctattaa cggtggtcac gcatcccttg aacccttcgc aacgcctgcc
    39961 gccggatcgc tattttactg aaatcaccgt cagccatcgg atggaggttg tgaaagagca
    40021 gattgatttg ctggcgcaaa aagccgactt ctttgtagag cacggcctgc tggcatcggt
    40081 caatattgat ggccctacgc tcatcgccct gcgtcagcaa ccaaaaatcc tgcgccagat
    40141 tgagcgtctt ccctggctgc gtttcgaact ggtggagcat atccgtctgc cgaaagattc
    40201 aacctttgcc tcgatgtgtg aatttggccc gctgtggctg gatgattttg gtaccgggat
    40261 ggcaaatttc tctgcactaa gtgaagtgcg ttatgactac atcaaaatcg cgcgagaact
    40321 gtttgtgatg ctgcgtcagt cgccggaagg ccgcacactc ttttctcagc ttttacatct
    40381 aatgaatcgc tattgtcgcg gggtgattgt cgagggcgta gaaacaccgg aagagtggcg
    40441 tgatgttcag aactcgcccg cattcgccgc acaaggctgg tttctttcac gcccggcacc
    40501 gatggaaacg ctgaatacgg cggttctggc gctataagct gcctcatttt ccgccgggct
    40561 ggactatctt taggactggc acaggaagaa tatgaggaag cgaacgatga gcaaggcagg
    40621 caaaataacc gctgcgattt caggggcttt cttgttgttg attgtcgtgg cgatcatttt
    40681 gattgcaact tttgactgga atcgactcaa accgaccatc aaccagaaag tctctgcgga
    40741 gttgaatcgt ccgttcgcta tccgtggcaa tctgggcgtg gtgtgggagc ggcaaaaaca
    40801 agaaactggc tggcgcagct gggtgccgtg gcctcatgta catgcagaag acatcattct
    40861 tggtaatcca ccggatattc ccgaagtcac gatggtgcat ttgccgcgag tagaggcaac
    40921 gctggcaccg ctggcgctgc tgaccaaaac ggtctggctg ccgtggatca agctcgaaaa
    40981 gcccgacgcg cgcctgattc gcctctctga aaagaacaat aactggacgt ttaatcttgc
    41041 caacgatgac aacaaagacg cgaatgcaaa gccgtcggca tggtcgtttc ggctggataa
    41101 tattcttttc gatcaagggc ggatcgccat tgatgacaaa gtaagcaaag cggatctgga
    41161 aatttttgtt gatccgttag gcaagccgct gccattcagc gaagttactg gatcgaaagg
    41221 taaagcggat aaagaaaagg tgggcgatta catttttggc ctgaaggcgc agggacgtta
    41281 taacggtgaa ccgctcacgg gtacgggaaa aataggcggt atgctggcgc tgcgtggcga
    41341 agggacgccg tttccggtac aggctgattt ccgttcaggt aatacccgtg ttgcttttga
    41401 tggcgtcgtg aatgacccaa tgaagatggg cggtgtcgat ttacggctta aattttctgg
    41461 cgattcactg ggtgatctct atgaactgac gggcgttctg ctgcccgata ccccgccgtt
    41521 tgaaacggat ggtcggctgg tagcgaaaat cgacactgaa aaatcgtcgg tctttgatta
    41581 tcgcggtttt aatgggcgaa ttggcgatag cgatatccac ggttctctgg tctacaccac
    41641 cggaaagcca cgaccaaaac tggaaggtga tgtcgagtcg cggcaattgc ggctggcgga
    41701 cctgggaccg ttgattggcg ttgattccgg gaaaggggca gaaaagtcga aacggtctga
    41761 acagaagaag ggcgaaaaaa gcgttcagcc tgcgggcaaa gtgctgcctt atgaccgctt
    41821 cgaaaccgat aaatgggacg ttatggatgc cgatgttcgc ttcaaagggc ggcgcattga
    41881 gcatggcagt agcctgccga ttagcgatct ttctactcat atcatcctca aaaatgctga
    41941 cctgcgcctg caaccgctga aatttggcat ggcgggcggc agcattgcgg cgaatattca
    42001 tctggaaggc gataaaaagc cgatgcaggg gcgggcagat attcaggctc gtcgactgaa
    42061 actgaaagaa ctgatgcccg atgtggaact gatgcagaag acgctggggg aaatgaacgg
    42121 tgacgcggaa ctacgcggta gcggtaactc ggtggcggcg cttttaggca acagtaacgg
    42181 caacctgaaa ctgttgatga atgacgggct ggtgagccgc aacctgatgg agattgttgg
    42241 gctgaatgtc ggcaactaca ttgtcggtgc gatatttggt gacgatgagg tgcgggtgaa
    42301 ctgcgcggcg gcgaatctga atattgccaa cggcgtggcg cgcccgcaga tttttgcttt
    42361 cgatactgag aacgcgttga ttaatgttac cggcacggca agttttgctt cggaacagct
    42421 ggatttgact attgatccgg agagtaaagg gattcggatt atcacactgc gttcgccgct
    42481 gtatgtgcgt gggacgttta aaaatccgca ggctggggtg aaagccggac cgctgattgc
    42541 ccgtggtgct gttgctgcgg cactggcaac gctggtaaca ccggcggcgg cgttactggc
    42601 actgatctca ccttccgaag gggaggctaa tcagtgtcgg acgatattgt cgcagatgaa
    42661 gaagtgatga taattgccgg atgtctgaca tccggcaatt aaacagattt agatttttgg
    42721 caaagtgtgc tttgttcatg ccggatgcgg cgtgaacgcc ttatccggcc tacaaatcct
    42781 tgtgaattca atatattgta tggaaattca gtcctgataa gcgtagcgca tcaggctttt
    42841 cacacttatt acaacgactg atgtcgcgtc tcatgggtca gcagcagggc gattaacgtc
    42901 aagccagcca tcgccgccag atataacccc accgcaccta acccgtagtt agcctgcaac
    42961 caggctgcga tatacggcgc aacggaagcc ccgagaatcg acgatacgtt gtaagagaac
    43021 gatgctccgg tgtaacgcac ttctgtcgga aacagctctg gtaacagcgc acccatcggc
    43081 ccgaaggtca gccccatcag acttaacccc agcagcagga aggcaaaaac cagaatcggg
    43141 ttgccagaac cgagcagcgg gttaaaggcg aacagcgcga acaggatgat cagcgtggtg
    43201 atgatcacca tgcttttacg gcgaccaaag gcatcagcca gtaatccggc gactggcacc
    43261 atcacgccaa aaccaataac tgccatcatc agcatccaca acacttcgtt acgcggcagg
    43321 ccaagcccaa ctggcgcggc ggcggtactg aaggtcatag agtagaccgt catgatgtaa
    43381 aacagcgtat aggttgccag cataatgaac gtacccagta cggttacgcg aacatgtttg
    43441 gtcagcagcg tacccagcgg gatcttcacc tgttttttcg ctttagcgac tttctcaaac
    43501 accggcgact catgcagcga cacgcgaaca tacaggccga taatgaccag caccgccgag
    43561 aagataaatg gcacacgcca gccccagctc ataaactgct catcggtcag tagccaggaa
    43621 agcagcaaga aagtgccgtt ggcaaagaag aagccaatcg gtgcgcctag ctgtgggaag
    43681 gagccataca gtgcacgttt gcgcggtggg gcgttttcag tcgccagcag cgccgcgccg
    43741 ccccattcac cgcctaagcc cagaccctga ccaaatcgag ccagcgccag cagcagtggg
    43801 gcgaaaatac caatcgtggc atagcccggc agcagaccaa tcaccacggt cgaaatcccc
    43861 atcgttagca acgaggcgac cagcgtcgct ttacgcccaa cgcgatcgcc aaaatgacca
    43921 aaaacggcag agccaatggg gcgcgcgacg aaggcgatgg cgaaggtggc gagcgactgt
    43981 agcgttgctg ccgttggatc gccctgcggg aagaagatat gcggaaacac aataacggcc
    44041 gcagtggcgt aaatgtaaaa gtcgaagaac tcaatggccg tgccaatgag agaggcgaca
    44101 aggactttat tacgcgagtt gatcggcgtg tattcttgtt cgtggtcgag tgttgtggct
    44161 gttgcttgca taatcttttc ttattttttg gctaacgaat agccatatta cgcacagcaa
    44221 aagtgatatt tcaatgtgta acaaatcgcg gaaatggtca aaaaccaggc aaaaagggga
    44281 taatgtttag gccagtgatt tttcttctat gaaatgcttc acataatttt ataaatagtg
    44341 gataaaactg gtttcaggtt aaataaaagt taccaactgg atttatatgc tggggttatg
    44401 agttggagtg aaattgtttg ctcccaaata gatggctgaa gaggctctgg ggaagagttt
    44461 caagaggtta tgtgcgcatt atagccggat gcgatgctgg cgcatcttat ccggcctacg
    44521 gggagccagg ccgaataaaa tcgtatgccg gataaggcgt ttacgccgca tccggcctat
    44581 tttaaggctg cgttttccct ggcattcgcg ggtcgtcttt atattcggcg gtggcaatcc
    44641 acgccgcgca aaacagcgtc aaacgggcga agaagtagaa aaatgccatc agccccagca
    44701 cggaaccaaa tgctgcgcca gagggggatt tcatcagcga tggcagggtg taggtcatca
    44761 cgattttaat cacttcaaaa ccaatagcag ccagaaatgt tccgcgaatc agggcttttt
    44821 tgcgcggacg gtggcgcggc aaccgccaga agatccagaa gaaaagcaga tagttggcga
    44881 agatggaaat cgccaggcca atcaatcgcc acgtcggttt cagccactca atgctgttca
    44941 ggtgcagggc gctaataatc atttgctgcg ccgaaccggc aaccgaggtg atcgaaagcg
    45001 tcacaatcag cgcaatcaac aaaccaatca gtgaaataaa atcacgcaga tattttaccc
    45061 agaacttctc ctgatcttgc ggcgagcgtt cccagacatc gcgcgactgg gcacgaatcg
    45121 cttcacgcag gttacccatc cagttgatgc cggaataaag tgccaccgcc aggccgacaa
    45181 gccctacagt cgtacgctgc tgaacggcgg tgttgatggt gtttttcaac gtggcggcta
    45241 gtgtcggatc gctgatgttt tgcagaattt tgtcgaagat atcctgtagc aacatcggat
    45301 gggaggccag cacaaagccc cctgcggcaa acgacaccat caaaatcggg atcatcgaca
    45361 aaaatgagaa ataggtgata gccgcaccaa actggttacc cagccgatcg ttaaagcgtt
    45421 ctgtcgcgcg aataaggtgc gcaataaccg gttggcgttg gactttttcg gcagtggtgg
    45481 tgacggtttc cagcgcctgg ctaactttgc cgcctttctc gctattatcc aactgcttaa
    45541 tcggctcgtg ctccagatcc tgggtgggac gtttgatctc gttttcctgc gtcatccgct
    45601 cttttgtcct tctgtcgtgg tcattcttaa aaagtatagt cggtcagtcc aggtgctctt
    45661 tcattacgcc agccagccac tgcataaaca ggtttacacg ccgggaaagc tcccgacgct
    45721 gcgggtaaac cagcgaaagg gagagcggct cggcacggta gcctggcaac acttcaataa
    45781 gccgcccggc acgcagggct tcgcgcacgg caatgcgcgg gatctgaata atccccagac
    45841 cggcaagacc ggcggcgaga tagttttcgc tactgtttac cgtcaacatg ccgccagact
    45901 taaaccactg gacgccatta acgctggcaa cctcaaaacc taacggatgt acacccaggt
    45961 gcggtgtata acgcactatt gcgtgtgaag tcagatcatc ggggctttgc ggataaccaa
    46021 agcgcgtcag atagtgcgga ctggcacagt tgaccatggt tagtttgccg aggggacggg
    46081 cgataacgcc atcttccggt aacgcgccag tgcgtatcac gcaatcaaaa ccatcatgaa
    46141 gaatatctac cggacggtta tggctactca gttccagctc aattcccgga tgctgataga
    46201 gaaattccga cagacgcggc agtaacaggt ttttcgcgat tcctgacggg atgtcgatgc
    46261 gtaatttacc gctgatactg gtggcatcct gttgaaacag accgtccagt tcgctgagat
    46321 tactcaacac atcttttgcc cgttgataat aggtcatgcc ttctggcgtg agtttgaccc
    46381 gtcgcgtagt gcgctggaga agctgggtgc caagttgatg ttccagtgcc tgtatctggc
    46441 gcgaaacact tccctttggc aaagcaaaga aatccgccgc gcgggaaaaa ctttccagct
    46501 ccgcgacttt gatgaacaac tgcattgcgt gaattttatc catttctttg accgattgtt
    46561 gttcacagcg aaacagtaac gcgcatatcg cattgtttat tgttttttct aacatctaat
    46621 gccgggtttc acaatgttgt catgacgaat gtggcgattt ctcagcagga aaagggcgga
    46681 atagctttat gagaaaggtg aatcccctta aacagagcgt atttttcagt gattcccttt
    46741 aaagtgaata gaggacgtat tggctatttg tgcttatttt tgtctcattg ccatttgtta
    46801 ttttggcttg tagtcgcgga atttcatttt taattgcatg cgtactaacc accaatataa
    46861 ggaaatcctc aaaattgttt taaatagtgc cctcgacatg taatttcagg cgtttagctg
    46921 taactattta tatcggtaac tattttctac gcgtcatctc tgtagaatgg ccaccgccaa
    46981 atggcgaatt cttatttttt ctcgcgggta atacccttcc actacttaag tctttttaac
    47041 gtattcaggt ggagattcag ttatgcaaat agtcatgttt gacaggcagt caatatttat
    47101 tcatggaata aaaatcagtt tacagcagcg tattccagga gtgagtattc agggggtcag
    47161 tcaggcagac gagttatggc aaaagctgga aagttaccct gaagccttag ttatgctcga
    47221 tggcgatcag gatggtgagt tttgctactg gttgctgcaa aaaaccgtgg tgcagtttcc
    47281 tgaggttaag gtgttaatca cggcgacgga ttgcaataaa cggtggttac aggaagttat
    47341 ccattttaat gtgctggcca ttgtgcctcg cgattcaacc gtcgagacat ttgcgttggc
    47401 ggtaaatagt gcggcgatgg ggatgatgtt tctgcctgga gactggcgga cgacgccgga
    47461 aaaggacatt aaagatctca aatcgttaag cgcccgccag cgggagattt taaccatgtt
    47521 agcggcgggc gaatcaaata aggagatcgg cagagcgctg aatatcagta ccggaacggt
    47581 aaaagcgcat ctggaatccc tgtatcgccg tctggaagtg aaaaatcgca cccaggcggc
    47641 aatgatgtta aatatctcct cctg
//
LOCUS       DAZPCS010000016        46515 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZPCS010000016 DAZPCS010000000
VERSION     DAZPCS010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46515
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.016"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(68..1927)
                     /gene="gss"
                     /locus_tag="ACHUQ7_000859"
     CDS             complement(68..1927)
                     /gene="gss"
                     /locus_tag="ACHUQ7_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFR1134384.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2132..2998
                     /gene="yghU"
                     /locus_tag="ACHUQ7_000860"
     CDS             2132..2998
                     /gene="yghU"
                     /locus_tag="ACHUQ7_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFR1134385.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3027..3108)
                     /locus_tag="ACHUQ7_000861"
                     /pseudo
     CDS             complement(3027..3108)
                     /locus_tag="ACHUQ7_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3121..3369)
                     /gene="hybG"
                     /locus_tag="ACHUQ7_000862"
     CDS             complement(3121..3369)
                     /gene="hybG"
                     /locus_tag="ACHUQ7_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFR1134386.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3503..3844)
                     /gene="hypA"
                     /locus_tag="ACHUQ7_000863"
     CDS             complement(3503..3844)
                     /gene="hypA"
                     /locus_tag="ACHUQ7_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFR1134387.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3837..4325)
                     /gene="hybE"
                     /locus_tag="ACHUQ7_000864"
     CDS             complement(3837..4325)
                     /gene="hybE"
                     /locus_tag="ACHUQ7_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFR1134388.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRGE
                     NA"
     gene            complement(4318..4812)
                     /locus_tag="ACHUQ7_000865"
     CDS             complement(4318..4812)
                     /locus_tag="ACHUQ7_000865"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFR1134389.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4812..6515)
                     /gene="hybC"
                     /locus_tag="ACHUQ7_000866"
     CDS             complement(4812..6515)
                     /gene="hybC"
                     /locus_tag="ACHUQ7_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFR1134390.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6512..7690)
                     /gene="hybB"
                     /locus_tag="ACHUQ7_000867"
     CDS             complement(6512..7690)
                     /gene="hybB"
                     /locus_tag="ACHUQ7_000867"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFR1134391.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7680..8666)
                     /gene="hybA"
                     /locus_tag="ACHUQ7_000868"
     CDS             complement(7680..8666)
                     /gene="hybA"
                     /locus_tag="ACHUQ7_000868"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFR1134392.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8669..9787)
                     /gene="hybO"
                     /locus_tag="ACHUQ7_000869"
     CDS             complement(8669..9787)
                     /gene="hybO"
                     /locus_tag="ACHUQ7_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFR1134393.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGSAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9976..10263)
                     /gene="yghW"
                     /locus_tag="ACHUQ7_000870"
     CDS             complement(9976..10263)
                     /gene="yghW"
                     /locus_tag="ACHUQ7_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFR1134394.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10382..11269)
                     /gene="yghX"
                     /locus_tag="ACHUQ7_000871"
     CDS             complement(10382..11269)
                     /gene="yghX"
                     /locus_tag="ACHUQ7_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFR1134395.1"
                     /translation="MPRLTAKDFPQELLDYYDYYVHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVG
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            11426..12466
                     /gene="gpr"
                     /locus_tag="ACHUQ7_000872"
     CDS             11426..12466
                     /gene="gpr"
                     /locus_tag="ACHUQ7_000872"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFR1134396.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12506..13000)
                     /locus_tag="ACHUQ7_000873"
     CDS             complement(12506..13000)
                     /locus_tag="ACHUQ7_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFR1134397.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLKWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13191..14075
                     /gene="yghA"
                     /locus_tag="ACHUQ7_000874"
     CDS             13191..14075
                     /gene="yghA"
                     /locus_tag="ACHUQ7_000874"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFR1134398.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPIPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASVITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(14347..14772)
                     /gene="exbD"
                     /locus_tag="ACHUQ7_000875"
     CDS             complement(14347..14772)
                     /gene="exbD"
                     /locus_tag="ACHUQ7_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFR1134399.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14779..15513)
                     /gene="exbB"
                     /locus_tag="ACHUQ7_000876"
     CDS             complement(14779..15513)
                     /gene="exbB"
                     /locus_tag="ACHUQ7_000876"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFR1134400.1"
                     /translation="MGNNLMQTDLSVWGMYQYADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15765..16952
                     /gene="metC"
                     /locus_tag="ACHUQ7_000877"
     CDS             15765..16952
                     /gene="metC"
                     /locus_tag="ACHUQ7_000877"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFR1134401.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            17092..17751
                     /gene="yghB"
                     /locus_tag="ACHUQ7_000878"
     CDS             17092..17751
                     /gene="yghB"
                     /locus_tag="ACHUQ7_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFR1134402.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGSWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17791..18690)
                     /gene="yqhC"
                     /locus_tag="ACHUQ7_000879"
     CDS             complement(17791..18690)
                     /gene="yqhC"
                     /locus_tag="ACHUQ7_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFR1134403.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18884..20047
                     /gene="yqhD"
                     /locus_tag="ACHUQ7_000880"
     CDS             18884..20047
                     /gene="yqhD"
                     /locus_tag="ACHUQ7_000880"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFR1134404.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGTGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            20152..20979
                     /gene="dkgA"
                     /locus_tag="ACHUQ7_000881"
     CDS             20152..20979
                     /gene="dkgA"
                     /locus_tag="ACHUQ7_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFR1134405.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGKIAKLDQGKRLGPDPDQFGG"
     gene            21179..22105
                     /locus_tag="ACHUQ7_000882"
     CDS             21179..22105
                     /locus_tag="ACHUQ7_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFR1134406.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            22156..22413
                     /gene="yqhH"
                     /locus_tag="ACHUQ7_000883"
     CDS             22156..22413
                     /gene="yqhH"
                     /locus_tag="ACHUQ7_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFR1134407.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22456..24675)
                     /locus_tag="ACHUQ7_000884"
     CDS             complement(22456..24675)
                     /locus_tag="ACHUQ7_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708831.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFR1134408.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTLGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VIVQPPRQKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEGVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(24786..26198)
                     /gene="ftsP"
                     /locus_tag="ACHUQ7_000885"
     CDS             complement(24786..26198)
                     /gene="ftsP"
                     /locus_tag="ACHUQ7_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFR1134409.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(26273..27010)
                     /gene="plsC"
                     /locus_tag="ACHUQ7_000886"
                     /gene_synonym="parF"
     CDS             complement(26273..27010)
                     /gene="plsC"
                     /locus_tag="ACHUQ7_000886"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFR1134410.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(27244..29502)
                     /gene="parC"
                     /locus_tag="ACHUQ7_000887"
     CDS             complement(27244..29502)
                     /gene="parC"
                     /locus_tag="ACHUQ7_000887"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFR1134411.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(30048..30530)
                     /locus_tag="ACHUQ7_000888"
     CDS             complement(30048..30530)
                     /locus_tag="ACHUQ7_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFR1134412.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDAPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(30583..30975)
                     /gene="ygiW"
                     /locus_tag="ACHUQ7_000889"
     CDS             complement(30583..30975)
                     /gene="ygiW"
                     /locus_tag="ACHUQ7_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFR1134413.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSVTQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            31127..31786
                     /gene="qseB"
                     /locus_tag="ACHUQ7_000890"
     CDS             31127..31786
                     /gene="qseB"
                     /locus_tag="ACHUQ7_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFR1134414.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            31783..33057
                     /gene="qseC"
                     /locus_tag="ACHUQ7_000891"
     CDS             31783..33057
                     /gene="qseC"
                     /locus_tag="ACHUQ7_000891"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFR1134415.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNGPGVTPEALARIGERFYRPPGQTATGSGL
                     GLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(33103..33435)
                     /locus_tag="ACHUQ7_000892"
     CDS             complement(33103..33435)
                     /locus_tag="ACHUQ7_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HFR1134416.1"
                     /translation="MLKQKIKTIFEALPYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWLYVFSLVICLVCGWWLF
                     WGRFIFCY"
     gene            33754..34335
                     /gene="mdaB"
                     /locus_tag="ACHUQ7_000893"
     CDS             33754..34335
                     /gene="mdaB"
                     /locus_tag="ACHUQ7_000893"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFR1134417.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGSLVQDKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            34366..34680
                     /locus_tag="ACHUQ7_000894"
     CDS             34366..34680
                     /locus_tag="ACHUQ7_000894"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFR1134418.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(34728..36620)
                     /gene="parE"
                     /locus_tag="ACHUQ7_000895"
     CDS             complement(34728..36620)
                     /gene="parE"
                     /locus_tag="ACHUQ7_000895"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFR1134419.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQGLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(36649..37230)
                     /gene="yqiA"
                     /locus_tag="ACHUQ7_000896"
     CDS             complement(36649..37230)
                     /gene="yqiA"
                     /locus_tag="ACHUQ7_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFR1134420.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(37230..38057)
                     /gene="cpdA"
                     /locus_tag="ACHUQ7_000897"
     CDS             complement(37230..38057)
                     /gene="cpdA"
                     /locus_tag="ACHUQ7_000897"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFR1134421.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(38082..38504)
                     /locus_tag="ACHUQ7_000898"
     CDS             complement(38082..38504)
                     /locus_tag="ACHUQ7_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFR1134422.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(38505..39134)
                     /gene="nudF"
                     /locus_tag="ACHUQ7_000899"
     CDS             complement(38505..39134)
                     /gene="nudF"
                     /locus_tag="ACHUQ7_000899"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFR1134423.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            39339..40820
                     /gene="tolC"
                     /locus_tag="ACHUQ7_000900"
     CDS             39339..40820
                     /gene="tolC"
                     /locus_tag="ACHUQ7_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFR1134424.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLEREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            40968..41639
                     /locus_tag="ACHUQ7_000901"
     CDS             40968..41639
                     /locus_tag="ACHUQ7_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFR1134425.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            41645..42805
                     /locus_tag="ACHUQ7_000902"
     CDS             41645..42805
                     /locus_tag="ACHUQ7_000902"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFR1134426.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(42843..43631)
                     /gene="ygiD"
                     /locus_tag="ACHUQ7_000903"
     CDS             complement(42843..43631)
                     /gene="ygiD"
                     /locus_tag="ACHUQ7_000903"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFR1134427.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            43774..44547
                     /gene="zupT"
                     /locus_tag="ACHUQ7_000904"
     CDS             43774..44547
                     /gene="zupT"
                     /locus_tag="ACHUQ7_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFR1134428.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQLLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(44605..44775)
                     /gene="yqiD"
                     /locus_tag="ACHUQ7_000905"
     CDS             complement(44605..44775)
                     /gene="yqiD"
                     /locus_tag="ACHUQ7_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFR1134429.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(45036..45689)
                     /gene="ribB"
                     /locus_tag="ACHUQ7_000906"
     CDS             complement(45036..45689)
                     /gene="ribB"
                     /locus_tag="ACHUQ7_000906"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFR1134430.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(45793..45941)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            46063..46353
                     /gene="ubiK"
                     /locus_tag="ACHUQ7_000907"
     CDS             46063..46353
                     /gene="ubiK"
                     /locus_tag="ACHUQ7_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFR1134431.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTEIKKQPDPETLPPTL"
BASE COUNT        11566 a        12419 c        11584 g        10946 t
ORIGIN      
        1 tgaatatttt gattaatccc cgtactaatt attcttcata atcagtacgg ggttgctaca
       61 acatgtatta ctttttcacc acaattaacg gttcaatatc actctctttt ttgatgacca
      121 gtgattcatc accgcgcaaa cacgtcccac cgtagttgcc gccaacggtg aaggtacata
      181 cctgaatgta tttaccgtcc actttcggca aacaccacag ttgctgatag atgtttttct
      241 gctcggcaaa tttaccgctg gttttgtcca gcacctcttc atgatggctg accaggtcga
      301 tattgctacc acagcgaccg gcgatcggtt tcactgcgta acctgttttc accaattcat
      361 cattaacagt gaaatcggta tccagcaggt aacgatggtg cgggaacagc gaccagagga
      421 tcggcagaat cgctttgttg ccggggatca ccgtccacag cggctcaaag accagcactt
      481 ccgggcgcag caatacgtcg ataagacgca cttcgttttg cggatgaccg gtacggattg
      541 gcaccgcagc aaactcacgg tcgctaactt cacgaatctg atcaaacgcg gtttcccacg
      601 cccaggtttt ccacacgcag ttaaccagtc gcccttcccc atcaatcagt tgcccggcag
      661 catcccagcc cagttcatcc agcccgcgca agatacgcgt ttcaaagccc gcctggtgca
      721 gcgcctgctc cataaactgc gcgtgatagt tttcctcgat atctttgtcc tgcatgatat
      781 ggacaaacgg acgtgcacga ctgtgtttcc aggcaccagc cagttcgtta atcagccctt
      841 ccgccggatt gaagccgttg cctttatagc cctgctccgc ccaacgttcg aggatcaagc
      901 ccgcttcggt atgacaggag gcggagtcgg cgttgtattc gtaaaccttc aggccacgct
      961 catccatgca gaaatccata cgaccagtga tcatatggtg acggcgacgc tgccaggaga
     1021 gacgcaaacg tggccagagg attttcggaa tgtcgaacag cgccagcagg ttgtcatctt
     1081 tcagcacctt gtcggttgcg tgaagataca tcagatgcag ctcgttggtg gctttaatta
     1141 gctcctgctc ggcactctcg gtaatggtgt agtaatgata cggatcctga ttgatcacct
     1201 gaccgttggc ctgcacatag gcgttttgta gcggatcttt ttcatccagc catttaccgt
     1261 caaactggcc tttgttttcc agacgcgctc cactgatttt cagcagctcg cctgcaattt
     1321 ccggctgcgg taagctgtat tcagtatctt ccgtctggat catccagccc agaatggtgg
     1381 tgtcatcaaa agtgtcttta agggtatagc agccgttttc gaccaccatc tccagctcgc
     1441 gcgtccactg ttgcccttgc ggcaacgggg aatgaatcac gttctgttcc gcaatacgga
     1501 ctttgttgcc atgcaattgg gtaatgatgg cgacatggcc agtgtcttta aattcaccgc
     1561 ctttatccca gataagaagc gcacccgcga ccggcgcacg cggcgagccg ttaggaaatg
     1621 cctgcaatgg caggatgttg tcattaacca cttctcgcag gaagcgcagc gagaaaatct
     1681 cccacgccat acccacgtca gtaaagacca caccgtaatt cagaaagaga aaacggcgag
     1741 caaattcaac gcattgccac ttgtggccca tatattcgtc gtcgatatag ctacggaata
     1801 cggcgtcatc ttcgtattcc tgcggatcga gagaactgta atctgaagag tagattgcta
     1861 ccccacctgg ggcgtagccc aataatgtcc cgaacggggc atcctggctg gtcgttcctt
     1921 tgctcatcac tttaccttta acaatacaac ctaagcagtt ggcgtaattg ttgtgctctg
     1981 attacctgct cagcgacatt aaccggacag aggtcagact aatcatacac ctcatcgcag
     2041 cggctggcgc aaagggttaa aaaattcaca ttctgtacag caagtgacct gctacactgc
     2101 ttcaacacta ccactcagaa ggcaactcac tatgacagac aatacttatc agcccgcgaa
     2161 agtctggacg tgggataaat ccgctggcgg cgcgttcgcc aatatcaatc gcccgttttc
     2221 tggtccgacg catgaaaaaa cgctgcccgt tggcaaacat ccattgcaac tttattcgct
     2281 gggaacgccg aacggtcaga aagtaacgat tatgcttgag gagctactgg cgctgggcgt
     2341 tactggtgca gagtacgacg cctggctgat tcgtattggc gatggcgatc aattctccag
     2401 cggctttgtc gaagtgaacc caaactcgaa gatcccggcg ctgcgcgatc atacgcataa
     2461 tccgccgatc cgcgtgtttg aatctggttc gatcctgctt tatctggcgg agaaatttgg
     2521 ctacttcctg ccgcaggatc tggcaaaacg gactgaaacg ttgaactggc tgttctggtt
     2581 acagggtgcc gcgccgttcc tcggcggtgg ttttggtcac ttttaccatt acgcgccggt
     2641 gaaaattgag tacgccatca accgctttac catggaagcc aagcgcctgc tcgacgtgct
     2701 ggataagcaa ctggcgcagc ataagtttgt tgcgggcgat gagtacacca ttgcggatat
     2761 ggcgatttgg ccgtggtttg gcaacgtggt gctcggcggc gtgtatgatg ccgcagagtt
     2821 tctggatgcg ggaagttata aacatgtgca acgctgggcg aaagaagtgg gcgaacgtcc
     2881 ggcagtgaaa cgtgggcgta ttgttaaccg caccaacggg ccgctgaacg agcagttgca
     2941 tgagcgccat gacgccagtg attttgagac aaatacagaa gataagcgtc aggggtaagg
     3001 gttggtgttc gtcgcagcaa gccatccagg ccggataagg cgttcacgcc gcatccggca
     3061 atcgtgcaca atgcctgatg cgacgctgcc gcgtcttatc aggcctacaa aagctaatca
     3121 tcacgcgctg gtaatgtcgt aatccatttg gcgcagtgcg tctaatgtgg ctttggcttc
     3181 atcttcgtcg atgatgctca tggcaaatcc gacgtgtacc agcacccact ggcccagcag
     3241 atcggcaggg ttaccttcgc aaatcagggc gatattcacg tcgcgcttga taccacatac
     3301 ttcaacctgc gcaagctggt gaatatcttc accgacagcc agcacctggc ctggaacgcc
     3361 aatacacatt gttaactccg gttatttcga ttgccggaca aacgcgaaaa agaaccttta
     3421 gcgggtgttc cctctcaccc taaccctctc cccggagggg cgaggggacc gtccgtgcgc
     3481 tttttaggcg ttgtcctcag tcttattcaa cttcaatact tttgacgatc agcgaatcgc
     3541 cggtatcgac acgcaaccgc tcgccgtgac agagcggaca ctgcgcatcg tgctgatgaa
     3601 tctccaccac ctggctgcaa tcccagcacc aggcctgggc gggtttatag acgatatgta
     3661 aatcgcaccc ttgcgccacc gttccgtggc agacaatttc aaaactaaaa cggacggcgc
     3721 tctcctcaac gcaggagagc gcgccaattt ccagccacac ggcggtgacg cgcttaacat
     3781 cgtgctgctc cgcctgccgt tggataattt caacggcgct ctggcaaagc gacaactcat
     3841 gcattttcgc ccctcctgcg gccaaacagc agggcgcgac gccctgcgtg tggtacatcc
     3901 ggattggtga ctggcaacga aaggatcatt cgtgcgcagt catccgtcag gcgttggcct
     3961 tcttcaatcg acatgctgtg cgaaagcggc gacatcagcg aacaggagag gtattgcgaa
     4021 acaccgtcca gttcgccaac ggtaaaggtc atggtgccat acggcagttg cagaccaatt
     4081 ttttcactca ctttgcgcag cggccagagt tgatccgggc cggggaagat aactgcactc
     4141 agcatccacg gggtgatcac acaccccgtc cactgacctt cgaacagcgt aaaatcagaa
     4201 acatacaccg gcattgaagg atgcagaaaa gagagatcgt gcatcgaacg ccgggcaatt
     4261 tcttcaaacg ctgcctgtac ttgcgccttc ggggaggtct ggaaacctgc tatctcttca
     4321 gtcatgaatc gcctcccgtg ggatggcttc cacgcctgat tcgcgcagcg cagccagaac
     4381 ctgctcaagc gcaggttcaa tcattgcttc aaccgtcggc gttaagccga tgtgtggctc
     4441 cagcgattcc gggatcacgc cgaccagggt cagctttttc ggaaactcac cggtgaagcg
     4501 cagggccgac aatacgtcgg ccaggccaag ctgatgcgga gagattttgt tggtaaacaa
     4561 cgccggaact tcttcatccc gcaggatcat catcgttccc ggagtgttct ttttcgacac
     4621 aatggcatcc gcaataatca gatgatcgcg atttgccatg tcgccaagca gctccattcc
     4681 cgccgtgccg ccatcgagga tctcaacata atccggcaga atgtatcgtt gctctaacgc
     4741 ttcgacaatc cgcacgccga tggcttcatc ggtcagcaaa atattgccga cccctaagac
     4801 taaaatacgc attacagaac cttcactgaa accacttcgt tgccgtcggc atccactacg
     4861 tgtaccgcac aggccatgca cgggtcgaag gagtgaatgg tacgcaccac ttccagcggt
     4921 ttattcggat cggcaaccgg tgtacccacc agcgactgct cgtaaggacc gacgtcatca
     4981 ttgaagttac gcggaccaga gttccaggtt gatggaacaa ccgcctggta gttgctgatg
     5041 ataccgtctt taataaccat ccagtgagag agcataccgc gcggcgcttc gaggaagcca
     5101 acacctttga actcacccgt tgccggaatg ttcggcttca caaaggtggt gtgatcgcct
     5161 ttgccgatat tggtgatcag tgcactgtat tggttttgca ggatatcctg caattcacag
     5221 cagtgaacgg tacgaccaat aatacggccc agcgtggagt gcagctgtgc cacttccagc
     5281 gtgttgccag tcagtttctg ataaatcgca acgatttcat tcagtttgtt ctgggtagat
     5341 tcgcgacctg ccgccagttt caccagcata ttagccagcg gacccacttc taccgttttg
     5401 ccgtagaaag tcggtgattt cacccaggaa tatttcccgt cgtcagacca accatcataa
     5461 gccggaatgg tggtaccttc ccacggcgcc tgtggcgctt cgtctttata ccaggagtgc
     5521 ttcgcgcttt cctgaatgcc tttgatcagg tattcatcgg aatgagaagt gatcggacga
     5581 tacgaggaca gatccgcatt ctcaatgtag cctcccggga acaggaagct gccgtttttg
     5641 ctgtcggtcg ggaattccgg cacgctcagg tagttcaccg cacctttacc gcgcgtcagc
     5701 cattccgggt agaacgcggc gataaccgcg gtatcaacct tataaacctg ctcaacaaag
     5761 tcgctcagtt tgtcgatgaa agacttgatg tacatcaggc gctcaaggtt caacacgccc
     5821 aacccgtcga ggttgattgg gttcgcgaca ccacccacag ccaggttctg aatgtgcggc
     5881 gttttaccgc ccagcagcgc cacgacgcgg ttagcgtcac gctggcactc cagcgcctgc
     5941 aggtagtgcg ctaccgcaat caggttcact tccggcggca gtttcatcgc cgggtgaccc
     6001 cagtagccat tggcgaaaat acccaactga ccgctggcaa ccagatcttt gatcttgttc
     6061 tgaactttag tgaactcttc cggactgttc agatgccagg tcgaaacgcc tttcagcatt
     6121 tcggaggctt tggttgggtc agcttgcagt gcagaagtga tgtccaccca gtccagcgcc
     6181 gaaagctgat agaaatgaac gatatggtca tgcgtggtgt gcgcagccag aatgatgtta
     6241 cggatgtatt gcgcgttaac cggaacgtcg atattcagcg cactttctgc cgcacgaacg
     6301 gaagacagcg cgtgagtggt agtacatacg ccacagatac gttgcacaat catccatgca
     6361 tcgcgcggat cgcggttttt cacgatctct tccatgccgc gccacatggt accggaagcc
     6421 catgctttcg aaacgacgcc attttcgatt tcgcaatcga tgcgtaaatg cccctcaata
     6481 cgggttaccg gatcaatagt aattctctgg ctcatgcttt gctcgcctca tgacgattat
     6541 gatcgttttg ttttaaagga ggaagtatcg gcagtagacg aatgagtacg atgtaagcgc
     6601 aaatctcaat agccacaaaa ccaatagaaa tcaacagttc ttcccaggtc gggaagtagg
     6661 cgtaaccgcc gcccgggttg aatgccacca acgaataggt cagacgccag gttgcacaac
     6721 ctaacagtgc gctcagtgct gacaggaaca gcatacggga atcattacgc agcttcgcca
     6781 cacgcagaac gaccagcggg aagagcatca gcaggacttc aatccagaac atcacggagt
     6841 agaagtcacc ggcaaacgct aacgacagct tgtcgcgata gataagctcg ccaaagcgca
     6901 gcccgatgaa aatcgccagc aacacactga tggtgttggt cagcttgacg aacaggctct
     6961 tttcatccgg accgttgcca cgcagacccg cctgcaccag tgaaccttca aagatgacaa
     7021 tcgagaagcc catgatgaac gccgtcagca gcgagaacag cggcaacatt tcatagctct
     7081 gccagagcgg atgcaccttg tagcccgccg agatcatcag cgaccccatt gaagactggt
     7141 gcatggtcgg cagcagcgca ccgagcgcga tgatgaagaa catcaccttg ttcagacgtt
     7201 gtagcgacac cttccagccc agacgttcaa acagtgccgg agcaaactcc agagccatca
     7261 cgccgatgta gatggtcata cagaccgccg tctcgaacag taccgagttc acgttgaagt
     7321 gacccggaat gtagaagtac ggcaggttcc agtagcgacc cacgtcgata gtgatcgaca
     7381 agccacccag tgagtaacca aacagactcg ccaacagcgc cggacgcacc agcggatggt
     7441 attgcccacg gttaaagacg tataccgccc acgccagcgc ccagccgcca caggcaaagc
     7501 cggtgccaat cagcaggtca aacgcgatcc acacgcccca cgggaagccg ccgttcaggt
     7561 cagagacaga gcccagaccg aacaccagac gcttcacaat caggagcata cagatgacga
     7621 ttaacggtcc aaaaatcatg accggtttac tgatgatttt gccgcccagc ggttgtggat
     7681 catgactcat gatcgtctcc tccgtcgtga tggtcgtttt tggtgttgcg acgaaccagc
     7741 acggttaagc ccgccagcac agccagtggt agcatcatgc ctttatacag ggtgtgttga
     7801 atattttcgg aacgcgcacc ggtagaaaga tcgtccagtt tcggcaggtc gagattttca
     7861 taaggcacac ccgtcagtac cagaacctga gtaccgccgc cctctttctc gccgtacaga
     7921 tgcggataat atttcggcac cgtatgcagg taagtgtcgc cagatttcag cgtctgacgc
     7981 ggatagtggt attcgctgcc aggcttcagc gccagacgtt ttttcgcctc cgccatcagc
     8041 tcttcacgcg taccgaaaat caccgcgccc gccgggcaca cttctacgca gccaggcaga
     8101 ccgcctttat cgagacgttc cacacctttc tggttgcaca gctcgcactt atgcagcgca
     8161 ccaaacgggt tgttgtagtc gtacttcggc acgttgtacg gacaggcgac catgcagtaa
     8221 cggcaaccgg tgcacacgtc tttgtcgtaa tggacaatgc cggttttcgg atcttttttc
     8281 agtgcagaga ccgggcacac agagacacag ttcggatcga cgcagtgcat acactgtttc
     8341 ttaatgtacg cgtagccgtt ctcctcctgg tctttgttga cccctgtgcc gctggtccac
     8401 acctgaatga tgttattggt atacggcgac agtttgtcgt tgttcgacca ggtctgttcc
     8461 ccttgcgggt tacgttcagg gaagttgata tcctgacact tggtgacgca agcctggcag
     8521 cctacgcaca aggtcgagtc gtacaacatc cccagcgatc ccggaattgg cgggcggttt
     8581 tcagcagccg catgactgac agacggcagc gcgcccgtca gcaatgcccc gcaggaggct
     8641 gctttaataa aattacgtct gttcacggtt attctccccg tgagtcagcg ttatctttct
     8701 tttgctgacg acccagttca cgcaccgcca tcacgctgac accggcaacc agcccaacca
     8761 caccgccgag caaaccaata gcgcctgcag agacgttgcc gccctcttta gcgttaacat
     8821 ccggtttctg tgaacgcgga gtctgatttt cgacgttggc aagctgatgg atgcctttat
     8881 ggaagccgat acctttttcg ttacagccat agcacgggtg accaatcgcc accggccaca
     8941 caccgccaac atcgcagaat tgcagcgttg agcagttgcc gtaagtttct ggccctttac
     9001 agccgaggtg gtacaagcac cagccttcgc ggtggccttc atcaccgaac tctttggcaa
     9061 aacgaccagc atcgaagtgc gggcgacgtt cgcagtgttc gtgaatcaga cggccatagg
     9121 cgaaggtcgg acggtttttg tcatccagtt tcggcggttt gccgtaagtg atgatgtgcg
     9181 caacggtcgc gaggaagttg tgcgggttcg gcgggcagcc cggaatattg ataacggttt
     9241 tgcctggcag aacttcttgc aagctgactg cgccagttgg gttaactcca gctgcggcaa
     9301 caccgcccca cgcggagcag gaaccaatag cgataatggc tgctgagcct tccgccgctt
     9361 tgcggatgtg atccacaatc ggctcaccgg caaccatgca ataaataccg ttatctttta
     9421 atgggatgga accatccacc actaacacat actgcccttt gtacttctcg agagcgttat
     9481 gtttgttctc ttcgacctga tgaccgaagg cggcggaaag cacttcgtga tattccagag
     9541 agatagtctc cagcacgagg ttttctaccg ttggatgcgt tgcacgaagc agagattccg
     9601 tacaaccggt gcactcctgc gcgccaatcc agataactgg cggacgctgc gggttagtaa
     9661 ccgattcggc catctctgca gcggctttgc tacttaaccc catggtggcg gctaatgctg
     9721 cacaaagctt catgaaatca cgacggttaa tgccgtgaga atggatgagg gtgttatctc
     9781 cagtcattta tagttattcc gttgcgagaa cctggcatat attttgcctc aatcgcaaaa
     9841 tcaataatgc gatcgatgcg ccatttacca cacatttatt atggttatcg atatcatgat
     9901 actgcggcgg ggcgctaaaa cgaagggaag aagcattagt gtaattaatt aaagcaagat
     9961 aatacgtatg tttgatcaat tttcgaggcg ataaccggcc ataaaaaagc gcaacgtaga
    10021 acaggaatta ttctcacgaa agaaatccat taccatctct ttatccagtc catagcggcg
    10081 cgtcagaata cccgcttccc aggcgctaag ctggcgatct ccggtctttt cgtacatcct
    10141 gtgtgagtag cctaatacaa aaccgcgttt ataatcggca cagaattttg tcacattaac
    10201 cgcactgtcg gcatgcgttg cttttaatcc cgccattaag cctttaccaa aatggttatt
    10261 catcgttcac ctcattcatt atatatcgcc atatataacg atattttagg caaagaacga
    10321 cttttgcgca cgctattttt tcatacttgt ttaagacgta atcccgtaag agataaacct
    10381 atcaggagag atatttatcg aaccatttca gtgtcctttg ccaggcaaga tcggcggcag
    10441 atttgtcata acggggcgtg gaatcattat ggaatccgtg attaaccccc ggatagatat
    10501 aagcctcata aaccttatta ttgtctttca acgccgcctc gtaaggaggc cagccctcgt
    10561 tgattcgggt gtccagttcc gcgaagtgga gtagtaaagg cgcttcaatc ttcgccacat
    10621 cggcagtggg tgcctgacga ccataaaacg gcaccgcgca ggccagttcc ggatacgcga
    10681 cagccgccgc gttcgatacg ccaccgccat agcaaaatcc ggtaataccc actttacctg
    10741 tcgcttgcgg atagcgttgc ataaactcaa ttgcggcaaa gaaatcattc atcagtttgg
    10801 ttggatcaac ctgctgttgc agctcacgac ctttatcatc atttcccgga taacctccaa
    10861 cggaacttaa tccgtcaggt gccagggcga tataccccgc cttcgccact cgccgtgcca
    10921 catcttcgat atacggattc agtccacgat tctcatgcac cacccccacg gctggcgttt
    10981 tgccgctcat ttttgcgggt ttcaccagat aaccccgtac ctcgccgtga ccatttggcg
    11041 aaggatacgt gatgtactca gcaacaatct ccgggtcggt aaactctacc tgagtcgcca
    11101 gcgcataatt tggcttgagc aaatcaaaca acgctaatgc cgtcatcccg cccaccgcat
    11161 acttcgccgc aaggttgagg aactcacgtt tcgaaatttt cccgtgaacg taatagtcgt
    11221 agtaatccaa caactcttgt gggaaatctt tggcggttaa acgcggcatc gttgcattcc
    11281 tcaactggtg tttttttaag caaagcataa gcgcgtgttt ttgcccagtt tttcgtcact
    11341 ctgtgagcca gactacggga tacgcgctgg cgaatcgcta aactagaaac attgtttcga
    11401 aattgaacgg tggaaaggag aggtcatggt ctggttagcg aatcccgaac gttacgggca
    11461 gatgcagtac cgctattgcg gaaaaagtgg tttacgcctg cccgcattat cgctcggttt
    11521 atggcacaat ttcggtcacg ttaacgcgct ggaatcacag cgtgcaatcc tgcgtaaagc
    11581 gtttgattta ggcattacgc actttgattt agccaacaat tacgggccgc ctccaggaag
    11641 cgcagaagag aactttggtc gcctgctgcg cgaggatttt gctgcttatc gcgatgaact
    11701 gattatctct accaaggctg gctacgatat gtggcccggc ccgtacggct ctggcggttc
    11761 acgtaaatac ctgctcgcca gcctcgacca aagcctgaag cgtatggggc ttgagtatgt
    11821 cgatatcttt tactctcatc gcgtcgatga aaatacgccg atggaagaaa ccgcctctgc
    11881 gctggctcat gcggtacaaa gcggtaaggc gctgtatgtc gggatctcct cttactcgcc
    11941 agagcggacg caaaaaatgg tcgagttgct gcacgagtgg aaaattccgc ttttaattca
    12001 tcaaccttcg tacaatttac tgaaccgctg ggtggataaa agcggcctgc tggataccct
    12061 gcaaaataac ggcgtgggct gtattgcctt tactcctctg gctcagggat tgctgaccgg
    12121 aaaatatctc aacggcattc cgcaagattc acggatgcat cgtgaaggga ataaagttcg
    12181 tggtctgacg ccgaaaatgc ttaccgaagc caacctcaac agcctgcgct tattgaatga
    12241 aatggcacag cagcgtggac aatcaatggc gcaaatggcg ttaagctggt tgctgaaaga
    12301 tgatcgcgtg acgtcggtat tgattggtgc cagccgcgcg gagcaactag aggagaacgt
    12361 gcaggcgctg aataatctga catttagcac cgaggagctg gcgcagattg atcagcatat
    12421 cgccgatggc gagctgaatc tgtggcaggc gtcgtccgat aaatgacctg ttaataacgg
    12481 gcgcgcgaac cgcgcccgaa tcagatcagt gattatgacg agtcagtcgg tcaagatagc
    12541 ccatcacaaa tgcagaaagc acaaacgtca gatggataat gacgtaccac atcagtttgt
    12601 tatccgggac atttttcgca tccataaaga cgcgcagtaa gtgaatggaa gaaattgcca
    12661 caatcgacgc tgctactttg tttttcagcg acgttgcgtc cattttcccc agccacttca
    12721 gcttctcttt gttctcggag atatccagct gtgagacgaa attctcataa ccggaaaaca
    12781 tcaccatcac cagcaaaccg ccaaccagcg tcatatccac cagcgacagc aacacgagga
    12841 tcaaatctga ttccgccatc gagaagatat tcggcagtac gtgaataatc tcctggaaga
    12901 acttcagcgc cagggcaact aacgccagcg aaaggccaaa gtacacgggg gcaagcagcc
    12961 agcgagaagc atacattgca ttttcaagaa aacgttccat acattcctgt cgtgtagtta
    13021 aaacgggcaa cagtatatcg caattgagta aacagcacgc aacagcaaat caacatatac
    13081 gttaaacctt tgtcgcataa ttctgaggtt tagggatctt cctggttccc ttatcaacgc
    13141 atcgagagcg tctaccctta aaggacgttg aacctgaagg gagaaaaacg atgtctcatt
    13201 taaaagaccc gaccacgcag tattacactg gtgaatatcc caaacagaaa caaccgacgc
    13261 caggcatcca ggcgaagatg acaccgatac cggattgcgg cgagaaaacc tatgttggta
    13321 gcggtcgcct gaaagatcgt aaagcactgg tgacaggggg cgattccgga ataggtcgcg
    13381 ctgccgccat cgcttacgcg cgtgaagggg ctgacgtggc gatcagttat cttcccgtgg
    13441 aagaagaaga cgctcaggat gtgaaaaaga tcattgaaga atgcggacgc aaagccgttc
    13501 tgctgccagg cgatttaagc gatgagaaat tcgcccgttc gctggttcac gaagcgcaca
    13561 aggcgttagg cgggctggat attatggcgc tggtcgccgg gaaacaggtt gccattccgg
    13621 atattgcaga cctcaccagc gaacagtttc aaaagacctt tgccattaac gttttcgcgc
    13681 tgttctggct aacccaggaa gcgatccccc tgctaccgaa aggtgcaagt gtcatcacca
    13741 cttcgtcaat ccaggcatac cagccaagtc cgcatttact ggactatgcg gctacgaagg
    13801 cggcgattct gaactacagc cgtggcctgg caaaacaggt cgcggagaaa ggtattcggg
    13861 tgaatattgt cgcgccaggc cctatctgga cggcactgca aatttccggc ggacaaacgc
    13921 aggataagat cccgcagttt ggtcagaaaa cgccgatgaa gcgtgcgggg caaccggcgg
    13981 aactggcccc tgtatatgtt tatctggcaa gtcaggagtc gagctacgtc accgcagaag
    14041 tgcacggcgt gtgcggcggc gagcatttag gttaaaaaaa tgcccggttg tgaaaagcaa
    14101 ccgggcatca ttgtgaattc actcttacct gatgtggcga aaacgcctta tccgcctaca
    14161 aacgcatgaa gattcaacat gttgcaatag attttgtagg cctgataagc gtagcgcatc
    14221 aggcattgag cacctaatgc cggatgcgtc gtgaacgcct tatccggcct acaaacgcac
    14281 gaagattcaa catgttgcaa tagattttgt aggcctgatg agcgtagcgc atcaggcaat
    14341 tctactttac ttcgctttgg cggtttcttc gccgaccaga cctatcttca ggtaacccgc
    14401 ctgatgcagc gtatccatta ccttcatcaa cgtctcgtaa tcgacggttt tgtccgctcg
    14461 gaagaagatg gtggtgtctt tcttgccttc ggttaacgca ttcaacgccg taatcattgt
    14521 ttcatcggtg accggatcgt taccgataaa catcgagttg tctgccttca ccgacagata
    14581 aaccggtttt tccgggcgcg gctgcggcgt gctggtagaa gcaggcaagt tcaccttcac
    14641 atctaccgtc gctaacggtg ccgccaccat aaagataatc agcagaacca acatcacgtc
    14701 gataaacggc gtcacgttga tatcatgcat ttcgccgtta tcgtcgaggt tttcgttaag
    14761 atgcattgcc atcggacatt atcctgcgcg taatttttgt gcgacacgaa ccggatgcgc
    14821 agcggcgctg gcttccagat ctaggtcacg gctttgcagc aacaatacct gcgctgcaac
    14881 atcacccagc atcgctttaa agccgccaat ctggcgtgca aatacgttat agataacgac
    14941 cgcaggaatc gctgccacga ggccgattgc cgttgctaac agagcttctg cgatacccgg
    15001 cgcaacgact gccaggttag tggtctgcgt ttgcgcgata ccaataaagc tgttcatgat
    15061 gccccagacc gtaccaaaca gaccaacgaa cggagaaatc gcgccgatgg ttgccagata
    15121 gccgttaccg cggcccattt gacgacccac tgcggcgacc cgacgctcca gacggaagct
    15181 ggtacgttct ttaatacctt cgttatcgtc gctgccttct gacagttcca gctcattctg
    15241 cgcttcattg agcaaatgca ggcttaagct tttgctacca aaatcagcgg cgatatcgtt
    15301 ggcctggttt aaggaacgtg cttcagccag cagttgctgc tcgcgcttaa ggcgacgctt
    15361 ctgattgaag aactctacgc tcttactgaa gaagattgcc caggtgacta cagaggccaa
    15421 aataagccca atcatcacgc acttaacgac aatatcggcg tactgataca taccccagac
    15481 ggaaaggtcc gtctgcatta aattattacc cactgtgtat ctccaggacg caagtcacaa
    15541 aatctgcgca taataatatc aaaacgacgt cgaattgata gtcgttctca ttactatttg
    15601 catactgccg tacctttgct ttcttttcct tgcgtttacg cagtaaaaaa gtcaccagca
    15661 cgccatttgc gaaaattttc tgctttatgc caattcttca ggatgcgccc gcgaatattc
    15721 atgctagttt agacatccag acgtataaaa acaggaatcc cgacatggcg gacaaaaagc
    15781 ttgatactca actggtgaat gcaggacgca gcaaaaaata cactcttggc gcggtaaata
    15841 gcgtgattca gcgcgcttct tcgctggtat ttgagagtat ggaagccaaa aagcacgcga
    15901 cgcgcaatcg cgccaatggc gagttgttct atggacggcg cggaacgtta acccattttt
    15961 ccttacaaca agcgatgtgt gaactggaag gtggcgcagg ctgcgcgcta tttccctgcg
    16021 gggcggcagc ggttgctaat tcgattcttg cttttgtcga acagggcgat catgtgttga
    16081 tgaccaacac cgcctatgaa ccgagtcagg atttctgtag caaaatcctc agcaaactgg
    16141 gcgtaacgac atcgtggttt gatccgctga ttggtgccga tatcgttaag catctgcagc
    16201 caaacactaa aatcgtgttt ctggaatcgc cgggttccat cactatggaa gtccacgacg
    16261 ttccggcgat tgttgccgcc gtgcgcagtg tggtgccgga tgccatcatt atgatcgaca
    16321 acacctgggc agccggtgtg ctgtttaagg cgctggattt tggcatcgat gtttctattc
    16381 aggccgccac caaatatctg gttgggcatt cagatgcgat gattggcact gccgtgtgca
    16441 atgcccgttg ctgggagcag ctacgggaaa atgcctatct gatgggccag atggtcgatg
    16501 ccgataccgc ctatataacc agccgtggcc tgcgcacatt aggtgtgcgt ttgcgtcaac
    16561 atcatgaaag cagtctgaaa gtggccgaat ggctggcaga acatccgcaa gttgcgcgag
    16621 ttaaccaccc tgccctgcct ggcagtaaag gacacgaatt ctggaaacga gactttacag
    16681 gcagcagcgg gctattttcc tttgtgctta agaaaaaact caatgatgaa gagctggcga
    16741 actatctgga taacttcagt ttattcagca tggcctactc gtggggcggg tatgaatcgt
    16801 tgatcctggc aaatcaacca gaacatatcg ccgccattcg cccacaaggc gagatcgatt
    16861 ttagcgggac cttgattcgc ctgcatattg gtctggaaga tgtcgacgat ctgattgccg
    16921 atctggacgc cggttttgca cggattgtat aacattgcca cttttggaca attttgcaga
    16981 cattttattg tgaaaagtct taaattgttg cgtccgggat caaggcgtcc cggacgattc
    17041 aggagtacaa taggcagata aaggcttaaa cgctgttcca caggaaagtc catggctgtt
    17101 attcaagata tcatcgctgc gctctggcaa cacgactttg ccgcgctggc ggatcctcat
    17161 attgttagcg ttgtttactt tgtcatgttt gccacgctgt ttttagaaaa cggcctgctg
    17221 cccgcctcat ttttgccagg cgacagcttg ttgatactgg caggcgcatt gattgcccaa
    17281 ggggttatgg attttctgcc tacgattgcg attctgaccg ccgcagcaag tctgggctgc
    17341 tggctaagtt atattcaggg gagctggtta gggaatacca aaacggtgaa aggctggctg
    17401 gcacagcttc ctgctaaata tcaccagcgc gccacctgca tgtttgaccg ccacggtctg
    17461 ctggcgctgc tggctggacg ttttcttgca tttgtccgta cgctgctgcc aaccatggcg
    17521 ggaatttccg gtctgccaaa ccgccgcttc cagtttttca actggttaag tggattgctg
    17581 tgggtcagcg tggtaaccag ttttggctat gccttaagta tgattccgtt cgttaaacgc
    17641 catgaagatc aggtaatgac gttcctgatg atcctgccaa ttgccttgtt aaccgctggc
    17701 ttgttaggca cgctgtttgt ggtgattaaa aaaaaatact gtaacgcctg acgattttcc
    17761 ccgttcccgg ctgctgtacc gggaacgtat ttaattcccc tgcatcgccc gcattcttgc
    17821 cgcatcttcc cccggcgtca caccgaagta acgtttaaac tcacggctaa attgcgatgc
    17881 gctttcatag ccgacgcgca tcgctgctgc gctggccttc atgccgtcat ggatgatcat
    17941 catccgcgcc ttatgcagac ggtaattctt caaatactgc aacggcgagg tgctggtgac
    18001 agacttaaaa ttatggtgga acgccgatac gctcatgttg gcttctgccg ccagttgctc
    18061 gacgctcagg ttttcggtgt atttattctc aattcgtttc agcacgcggc taatcagact
    18121 gaagtgagtc tggcgactga ccagcgccag taacgcgccg ccgcaaggtc cggtcagcac
    18181 gtagtacaga atttcgcgga tgatctgttt gccgagaata cgcgcatcca gtggtcgctc
    18241 catcacgtcg agtaaccgct ccgccgcgca taaaatctct tctgataacg tggcggagtt
    18301 aatcccgctg gctgccatcg acggctggaa atgctcatct tcgccaatgt ccatcaacag
    18361 ttcctgtaac tgcaaaatat cgacattgag acgcaaccct gccagcggca cctctgacgt
    18421 ggcataggtt tcgcactcaa acggcaacgg caccgtcagc agcaggtatt cattggcatc
    18481 ataacgaaac acgcgttcat tgatataacc gattttatgc ccggaaaaga gaattatgat
    18541 gccaggctcg tacatcaccg gtgtacgtgc gaaaggcgtc tcgccataca acaaacgcac
    18601 atcgggcaac agttctgaca aactattttc tttatttttc agtttattaa ctttatccgc
    18661 cagcaagcga caaatctctt cacgtttcat atcgcgtaat ttcttaggaa taatgcggca
    18721 atttgattgt gcgcaatttt gtagcatttc tccagcactc tggagaaata ggcaagacat
    18781 tggcagaaat gagcattgag agccagggcg ctggcgatca caatgaaaaa catcaggcag
    18841 atcgttctct gccctcatat tggcccagca aagggagcaa gtaatgaaca actttaatct
    18901 gcacacccca acccgcattc tgtttggtaa aggcgcaatc gctggtttac gcgaacaaat
    18961 tcctcacgat gctcgcgtat tgattaccta cggtggcggc agcgtgaaaa aaaccggcgt
    19021 tctcgatcaa gttctggatg ccctgaaagg catggacgtg ctggaatttg gcggtattga
    19081 gccaaacccg gcttatgaaa cgctgatgaa cgccgtgaaa ctggttcgcg aacagaaagt
    19141 gactttcctg ctggcggttg gcggcggttc tgtactggac ggcaccaaat ttatcgccgc
    19201 agcggctaac tatccggaaa atatcgatcc gtggcacatt ctgcaaacgg gcggtaaaga
    19261 gattaaaagc gccatcccga tgggctgtgt gctgacgctg ccagcaaccg gttcagaatc
    19321 caacgcaggc gcggtgatct cccgtaaaac cacaggcgac aagcaggcgt tccattctgc
    19381 acatgttcag ccggtatttg ccgtgctcga tccggtttat acctacaccc tgccgccgcg
    19441 tcaggtggct aacggcgtag tggacgcctt tgtacacacc gtggaacagt atgttaccaa
    19501 accggttgat gccaaaattc aggaccgttt cgcagaaggc attttgctga cgctaatcga
    19561 agatggtccg aaagccctga aagagccaga aaactacgat gtgcgcgcca acgtcatgtg
    19621 ggcggcgact caggcgctga acggtttgat tggcactggc gtaccgcagg actgggcaac
    19681 gcatatgctg ggccacgaac tgactgcgat gcacggtctg gatcacgcgc aaacactggc
    19741 tatcgtcctg cctgcactgt ggaatgaaaa acgcgagacc aagcgcgcta agctgctgca
    19801 atatgctgaa cgcgtctgga acatcactga aggttccgac gatgagcgta ttgacgccgc
    19861 gattgccgca acccgcaatt tctttgagca attaggcgtg ccgacccacc tctccgacta
    19921 cggtctggac ggcagctcca tcccggcttt gctgaaaaaa ctggaagagc acggcatgac
    19981 ccaactgggc gaaaatcatg acattacgtt ggatgtcagc cgccgtatat acgaagccgc
    20041 ccgctaagct ttttacgcct caaactttcg ttttcgggca tttcgtccag acttaagttc
    20101 acaacacctc accggagcct gctccggtga gttcatataa aggaggaacg tatggctaat
    20161 ccaaccgtta ttaagctaca ggatggcaat gtcatgcccc agctgggact gggcgtctgg
    20221 caagcaagta atgaggaagt aatcaccgcc attcaaaaag cgttagaagt gggttatcgc
    20281 tcgattgata ccgccgcggc ctacaagaac gaagaaggtg tcggcaaagc cctgaaaaat
    20341 gcctcagtca acagagaaga actgttcatc accactaagc tgtggaacga cgaccacaag
    20401 cgcccccgcg aagccctgct cgacagcctg aaaaaactcc agcttgatta tatcgacctc
    20461 tacttaatgc actggcccgt tcccgctatc gaccattatg tcgaagcatg gaaaggcatg
    20521 atcgaattgc aaaaagaggg attaatcaaa agcatcggcg tgtgcaactt ccagatccat
    20581 cacctgcaac gcctgattga tgaaactggc gtgacgcctg tgataaacca gatcgaactt
    20641 catccgctga tgcaacaacg ccagctacac gcttggaacg cgacacacaa aatccagacc
    20701 gaatcctgga gcccattagc gcaaggaggg aaaggcgttt tcgatcagaa agtcattcgc
    20761 gatctggcag ataaatacgg caaaaccccg gcgcagattg ttatccgctg gcatctggat
    20821 agcggcctgg tggtgatccc gaaatcggtc acaccttcac gtattgccga aaactttgat
    20881 gtctgggatt tccgtctcga caaagacgaa ctcggcaaaa ttgcaaaact cgatcagggc
    20941 aagcgtctcg gtcccgatcc tgaccagttc ggcggctaac gtgcaaattc tcccggtggc
    21001 ggtaatgttc cgctaccgga cttttcagaa atcatttatt cccctcgcgt cccgcccgtt
    21061 gttactcttc cttgttcagg aatgccaaat ataaggacat catcatgcag agccggaagc
    21121 tcttaaaaga acaactcatc tatatccggg ataaacgcaa cggagaggtg aaaaacagat
    21181 gaaaataata cttctgtttt tagcagccct ggcaagtttt accgtacacg cgcagcctcc
    21241 ttcacagacc gtagaacaaa cagtccggca gatttatcag aactataaat cagatgccag
    21301 tacgccttat ttcggtgaaa ccggagagcg ggcgataact tcagcgcgta ttcaacaggc
    21361 gcttaccctg aacgacaatc ttacactacc gggcaatatt ggctggctgg attatgatcc
    21421 ggtttgtgat tgtcaggatt ttggcgatct ggtgttagaa agcgtagcga taacccaaac
    21481 tgacgtcgat catgccgatg ccgttgtgcg ctttcgtatc tttaaagatg ataaagaaaa
    21541 gaccacacag acactgaaaa tggtggcgga aaatggtcgt tgggtcattg atgatattgt
    21601 cagcaatcat ggcagcgtct tacaagcagt taatagcgag aatgaaaaaa cgctggccgc
    21661 tttagcttcg ttgcagaaag aacagccaga atcctttgtt gctgaactct ttgaacatat
    21721 tgctgattat agctggccgt ggacgtgggt ggtttccgac tcttaccgcc aggcggttaa
    21781 tgccttctat aaaaccacct tcaagacggc caataatccc gatgaagata tgcaaataga
    21841 acggcaattt atttacgaca atccgatctg ttttggcgaa gagtcgctat tttcacgcgt
    21901 tgatgaaatt cgagtcctgg agaaaaccgc cgattccgcc cgcattcatg ttcgttttac
    21961 gctgaccaat ggcaacaatg aagagcaaga gctggtttta cagcggcgcg aaggtaagtg
    22021 ggaaatcgct gattttatcc gcccgaacag cggcagccta cttaagcaga ttgaggcaaa
    22081 aaccgccgcc agattaaagc aatgagctga attaaataac aattagccgg aacaataaat
    22141 aaaagggaac actatatgaa aacgattttc accgtgggag ctgttgttct ggcaacctgc
    22201 ttgctcagtg gctgcgtcaa tgagcaaaag gtcaatcaac tggcgagcaa tgtacaaaca
    22261 ttaaatgcca aaatcgcccg gcttgagcag gatatgaaag cactacgccc acaaatctat
    22321 gctgccaaat ccgaagctaa cagagccaat acgcgtcttg atgcacagga ctattttgat
    22381 tgcctgcgct gcttgcgtat gtacgcagaa tgataaaaaa atccccggca gcatgtcagt
    22441 tgccggggat tttttttaac gtccaaccgc cgctttaggg cgtttcttcg caccagcatt
    22501 caccggacga gattgcgtag acgacgcttt ttttgccgta gcaggcgtct gacgctgagt
    22561 cgccatcggc gtatgtttag tcaacgccgg acgggtattg cggttctggc gacgagcttc
    22621 acgcatctct tcaatggttg gcgcaggcac taagcaatcg cgacggctgc caatcagatg
    22681 ctttttgccc atcgcttcca gcgcctggcg gattaacggc cagtttgccg gatcgtggta
    22741 acgcaacaac gctttatgca aacgacgctg tttgtcgccc ttcggtacga agacgccttc
    22801 gctcttataa ccaatcttcg ccagcggttt tttcccggtg taatacatgg tggttgagtt
    22861 cgccagcggc gatgggtaga agttctgtac ctggtcgaga cggaaacgat gctttttcag
    22921 ccacagcgcc aaattcacca tatcttcatc acgcgtaccg gggtgcgcag agatgaaata
    22981 cgggatcaga tactgctctt tacctgcctg tttcgagtag gtatcgaaca gctctttaaa
    23041 gcggtcatag ctgcccatgc ccggcttcat catcttcgac aacggccctt cttcggtatg
    23101 ttccggagca atcttcagat aaccgccgac gtgatgggtc gccaactctt tgatatagcg
    23161 cggatcttcc acggcgatgt cataacgtac gcctgaggcg ataaggattt ttttaatgcc
    23221 tttcagatca cgcgcacggc gatagaggtt gatcgtcggt tcgtggttag tgtccatgtg
    23281 cggacaaata tccggataaa cgcacgacaa acggcgacaa gtttgttcag cgcgtggcga
    23341 tttgcagcgc aacatataca tgttggcagt tggcccaccg agatcggaaa tcacgcccgt
    23401 aaaacctgga acggtgtcgc ggatcgcttc gatctcatta atgatcgaat cttccgaacg
    23461 gctctgaata atgcgccctt cgtgctcggt aatagaacag aaagaacagc cgccaaagca
    23521 gccgcgcata atgttgaccg aaaaacggat catttcgtaa gccggaatac gggcattgcc
    23581 ataggccgga tgtggcacgc gcttgtacgg cagcgcaaaa acgctgtcca tctcttcggt
    23641 agaaagcggg atagcaggcg ggttgatcca cacatagcgg tcaccgtgtt tttgcatcaa
    23701 tgcgcgggcg cagcctgggt tggtttcgtg gtgcagaatg cgcgaagcat gggcgtacag
    23761 cactttatcg cccttcactt tctcgaaaga aggcagcaat acgtaggttt tttcccacgg
    23821 tttctggcgc ggtggctgca cgattacagc tttcgcttcc tgctttttcg gcgcaacagg
    23881 tttgttatcc gcacacggca aatcttcgcc atacggatgc gggattgggt cgattttccc
    23941 tagggtatca agacgggtgg aatccacacc gctccagcca ggcagcgcct cttttacgat
    24001 aattgcggta ttacgcacat cgcggatttc actaatcggc tcgcccatcg ccagacgatg
    24061 cgctacttcc accagcggac gttcaccgtt accaaacatc agcatgtcgg ctttcgaatc
    24121 taccagaacg gaacggcgca cggtatcgga ccagtaatca taatgcgcgg tacggcgcag
    24181 gctagcctca ataccgccga ggatcaccgg cacatctttc cacgcctctt tacaacgctg
    24241 ggtataaacc agcgtggcgc gatccggtcg cttacccgcg acattatctg gcgtgtaggc
    24301 atcatcatga cgtaaacggc gatcggcggt atagcggttg atcatcgaat ccatgttgcc
    24361 agcagtaaca ccaaaaaaca gattcggttt acccagacgc ataaagtcgt ctttgctgct
    24421 ccagtccggc tgggcgatga tcccgacgcg aaagccctgc gcttccagca tacgaccgca
    24481 aatcgccatc ccgaagcttg ggtgatcgac atacgcgtcg ccagtaacca aaatgatgtc
    24541 gcagctatcc cagccaagtt gatccatctc ttcacgagac atcggcaaaa acggtgccgg
    24601 tccaaaacag gcggcccagt actgcggcca ggagaacagg tcgcgatccg gttggatcag
    24661 ggagatagag ctcattttgc ttccagaaat gataaaaaaa taatcaaagg ccggggatta
    24721 taagccggaa cgaaagagaa atcgaaaggt attccatact cgccctcctc gggcgagtat
    24781 gaagattacg gtaccggatt gaccaacagt tgcccaatcg agccacggtc cgccatttcc
    24841 agcgtctgac tgttgaagta gaacgggaag tgcgcccagg aaggctgacc gaaataaaca
    24901 agcagctcca cttgtccgtc aacccaaacg gtatctttcc agcctctgtc ttccgggaac
    24961 ggcatcgcgc cattcacgtt acggatctgg aacattacgc cttcaatatg gaacgcttgc
    25021 ggctcgtccg cgcgtaccgt ccagcgttcc cacgttcctt gctgcgcggt gacatcaata
    25081 cggttgacgt cccacagctg tccattaata cccgggtcat cacccagact gatatcgcga
    25141 ctgcgaattg gcgaaccagc catgatttca gttggcagca agcgcatcgg aagactgtct
    25201 gtgaccagcg gcagaaggcc ggttgggcgc agcgttagca ccaaggtaga aaccagaata
    25261 ctggatggct caaagaagcc gcgaatacga tcaacaatgc tcgccgcttc accacaggtg
    25321 atcgacactt catcgccgtt gctcatatcc accagaatct cgcggcgctc gcccggtgcc
    25381 agcgaaagtt gcttcaccga tacaggagca ggcaggaatc cctgatcgcc agaaatcaca
    25441 tgtaacgggc gaccatcgct catctgtagt tgatagcgac gagagttcga cgcgttcagc
    25501 agtcgcaagc gcacccagcc acgcgagact tcaacgtacg ggctttgtac accgttaacc
    25561 agcagcgtat caccaacaaa gccgccgctt cccggttcgt tgtattctgg cgtaccaaag
    25621 ttatccagcc gtttatcctg gataatgacc ggaaaatcat ccacaccata atggttgggg
    25681 ataggcagcg acttgctgac ttcatcttcc accagccaca ttccggcaag gccgttatag
    25741 acctgctgag ccgtgcggtt gggagtattg gcgtgatacc acagagtagc tgcgttctgg
    25801 cgaataggca gtacgggtgc ccagtcagcg tttggcgaca tcatccgtgc cggaccgccc
    25861 atcagcgggc ctggtacctg tagcccggcc accgtcattg agacattttc tgtcaggcgg
    25921 ttgctgtaaa taagcttaac atcgtcgccc ttccagacgc ggatagtcgg ccccaggtaa
    25981 cgaccattga ttccccagac cgacgcgcgt gtccctggcg taaatgacca gtgcgcacgt
    26041 tgtacagtca taaacagcgg ttgcccacgg cgagattcaa gtagcggcgg aacgggtagc
    26101 ggttgctgtt gcccggctgc gctggccttc aggggaacag cgcctgcaca aagtgcaatc
    26161 cccgatgcct gaatgaactg acgccgactg agtgacatat ttgctccatg taaaactggc
    26221 taaagtaata ccgtgcagga aagtgttccc cgcaaaaata cagattgccg acttaaactt
    26281 ttccggcggc ttcgcgttct gcgacttctt tatcgagctc ggcgattttt tgttccatta
    26341 tcgaacgaca atgggcagcc agctcacgaa cctgatcttt gccatactga ctgacgtcaa
    26401 ttggcggcag catttcgaca atcaccagac cgttgtgcag tcgattaaga ttaatcttat
    26461 tcgaagttgt agagacgcac acgggaataa tcgggacgcc cgccgcaatt gccgcgtgaa
    26521 atgctccagt cttgaacggt agcaggccgc gaccacggct gcgggttcct tccgggaaca
    26581 tccagatgga aatacggcgt tttttgaaat gattcacgac ttccgcaatg gtgccgtgag
    26641 ctttagtgcg attgtttctg tcgatcaata agttgccggt taaccagtac aactgcccga
    26701 agaaggggat ccacagcaag ctctttttac ctaccgtcac cgtcggcggt tgcacaatgt
    26761 tcgatgctgt caccatgtca tagttgttct ggtggttagc gatatagata gcattgccgt
    26821 agctttcagc atctgcaggt ttgcggcact caactttcag gccgaacagt ggcgcaagac
    26881 ggccaaacat atgcccaaag gtggccacat gtttcgggtt acgcgggctg aaaaggcagt
    26941 aaatggagcc gaatacacag actaagatgc tgtaaatcac ggtaataata agacgaaaga
    27001 tatatagcat aacaccctct gaaggtctga gagactggca ttgtacgtca cctgaaatca
    27061 ggttagggca ttgttaaaag ccgctctctg gaaaagcgta ttgttaatcg caacgcatga
    27121 ataaacaacg gtttcaaggg aatttttatc gtttccgggg gttgaatacg ctgccggatg
    27181 acgacttaac gtttcatccg gcgttccttg ctagcgggag gaaacagcgc cctccccggc
    27241 atattactct tcgctatcac cgctgctggc acggcgagga gagtcgatct caacacgatc
    27301 gatacgctgc aaaccgcgca tcaacgtacc gcggcgtcca cgttcgccag tgactttctg
    27361 tagctcttcc ggacgcagtt taattttgcg tttcccaaca tgaatggtca gcgtgctttg
    27421 cggcggcaga acgtacagtt gcgccagacc atcctcgcca cgcgcggctt ctgccgatgg
    27481 aatgttgata atcttgttgc ctttgccctt cgacagctgc ggcagatcgc ttaccgggaa
    27541 catcaacata cggcctgcct gagtgattgc cagcagcata tcggaagcat cttcaatcac
    27601 caccggcggc ataacatggg cattttccgg taaggtgatc aaagccttac ccgcacggtt
    27661 acgcgctacc agatcgttaa aggtgcagac gaaaccgtaa cccgcatcgg aagccatcag
    27721 cagtttctga tcgtcgcttt ccatcagcat atggtcaacg gtcgccccag gcggcaacgt
    27781 taatttgccg gtgagtggtt cgccctgacc acgcgccgac ggcagcgtaa tcgggtcgat
    27841 ggcatagcta cgaccggtgg aatcaacaaa cactaccggt tggttactct tacctttcac
    27901 cgccgctttg aagctatcac ccgctttata attcaggccc ggcgcgtcga tatcatggcc
    27961 tttagcgctg cgtacccagc ccatctgcga cagcacaatg gtgacaggtt cagacggcag
    28021 catgtcgtgc tcgctcatcg ctttcgcttc ttcgcgttcc tgcaacggcg aacgacgatc
    28081 gtcaccgtag gcttgcgcgt ctgcctgcag ttctttcttc agcaggttat tcattttacg
    28141 ctcggaagcc aaaatgccct gcaactggtc gcgctctttt tccagctcac tctgctcacc
    28201 gcgaatcttc atctcttcca gtttggcaag atgacgcagt ttcagttcga ggatcgcttc
    28261 cgcctgggtt tccgtaaggc caaaccgcga catcagcgcc ggtttcggtt catcttcgtt
    28321 acgaatgatc tcaatcactt cgtcgatgtt gagaaacgcc accagcaaac cttccaggat
    28381 atgcaggcgc ttgaggactt tctccagacg atagttcagt cggcggcgca cggtatcgcg
    28441 acggaacacc agccattcgg agaggatttc cagcaggttt ttcaccgccg gacgaccatc
    28501 cagaccgatc atattgaggt taatgcgata gctcttttcc agatcggtgg tagcgaagag
    28561 gtggttcatc acctgatcca tatccacgcg gttggaacgc ggcacaatca ccagacgggt
    28621 cgggttctcg tggtcagatt catcgcgcag atcgtcaacc atcggcagct ttttgttgcg
    28681 catttgcgca gcaatttgct ccagtacgcg cgcacctgaa acctgatgcg gtaatgcgct
    28741 gataaccacc gcgccatctt ctttcttcca caccgcgcgc atacgcactg aaccacgtcc
    28801 gttctcgtag attttacgaa tctcggcgcg cgaagtgata atttccgctt cagtcggata
    28861 atccggcccc tgcacgatat ccagcagctg atcgagcgta gttttcggct ggtcgattaa
    28921 tgcgattgcc gcctgagcta cttcacgcag gttatgcggt ggaatatcgg tcgccatgcc
    28981 gacggcaata ccggtggtgc cgttaagcaa aatgtttggc agacgggcag gtagcatttt
    29041 cggctcctgc aaagtgccgt cgaagtttgg cacccagtca gccgtcccct gccccagctc
    29101 gctcaatagc agctcggaat atttcgacaa ccgggattcg gtgtaacgca ttgccgcgaa
    29161 cgatttcgga tcgtccggcg cgccccagtt cccctgacca tcaaccagcg gataacggta
    29221 agagaacggc tgcgccatca ggaccatcgc ttcataacag gcgctatcgc cgtgcggatg
    29281 gtatttaccc agtacgtcac cgacggtacg ggccgatttt ttaaatttgg cgctggcatt
    29341 caggcccagt tcagacatcg catacacaat gcggcgctga acaggtttca gaccatcacc
    29401 aataaacggc aacgcacggt ccatgatcac gtacatggag tagtttaagt aggcgttttc
    29461 cgtaaattca tgtagcgcaa ggcgctctgc catatcgctc attaattctg attcctcaac
    29521 ttattcgcca gccttcaaag gggcaatatt gccggagata ctacattatc tgccgcgtcg
    29581 agtcacagga gatcagcgtg aatagcgccg ccggacgtgc tccgacggcg ataaaggcat
    29641 caatgtgcct tgatatacaa ctctttgctg tagacttgat cggcatagag ggcagcacaa
    29701 ggttgcgccc atgccacacc actcggcatg ggggtccgct ccgggcgatt cgagaagttg
    29761 aggttatgcg agtccaggga agcctaaagc atcaaagtag tcgactgtaa gtatacggtt
    29821 cagcccagtt tagctgtgtc tccaccagtg ccccgcgcct cctgccataa gtgcggcttg
    29881 ctcatggata tccccgagac ggcttacggc gcaccggata ataaatttcg cgtatacatg
    29941 gtattcctta ccggagaaat tataagccca acaacaatac gcctgtgaca ttgttcgctc
    30001 aaacttatcg cgagtgattt ggctcacact ccggtaaaga ttccccatca cgccaacggc
    30061 acataaacat ccgtttccag tcgctgctct gtcacccctt cggcaagatt ggtgtagtga
    30121 aacagaatcg gtgctttacg cattttctcg ccgcttgcag gcagccagtg gcgaataatg
    30181 ccccatatcg tgtgtgaaat atcgtccagc tcgccaacgt ggcgggccac ggcataacgt
    30241 ccaccggtaa gttcaccatt gctcacacca taacgattat cgggaatcgg ttcgctaacg
    30301 ctgccgcaga tatcaaagcg aaacgcttcc ggtgcggtgg tggcaggggc gtcccaggcg
    30361 acgccaaaag tctggctttg gttaacagga gaaagccccg tctctttacg ccacatgata
    30421 aatttcgcca cactgtagtt gagcaactca gggctacagc ggtgcggcaa catcgccaca
    30481 gggattgatg ggaaatcgat aatgtttaca tccagtgtca ggtttgtcat tttgcctccc
    30541 tgcgaaatta ttcccgggaa cgcgtcccgg gagcggtaac aattacggat ttactttgcg
    30601 gatctgtttg acgtcaattt caacagaatt ccagtcttta tcgacttcac cctgaatctc
    30661 aaccgtatct ttcggcgtca ccgtcacacc gttccagcgt ttgtgatcga tatcaacatt
    30721 gatagtaccg ctggcatctt taaacacgta gagatcgtca gagatgcgtt caacgatatt
    30781 gccgcgcagg gttacccagg tgtcgtcacg cagggatttt gcgctttcta cagtcgttac
    30841 gctgccgttc ggcccctgaa atcctccggc ctgactttgc gttaccgatg ggccagaaaa
    30901 accgccctgc tctgctgcca tcaccggtgc actgcacagg gccattactg cgattactgc
    30961 tgcgaatttt ttcatgttta ttactccctt taatgtctgt ttccgagcat ttaacaagat
    31021 agtccttaac aacttcttaa gggaaaaaaa taaaatttaa tgctgtacag agcgcgttac
    31081 aacacggttt actggcagca aatacggtta tcgcagggat gaaaaaatgc gaattttact
    31141 gatagaagat gacatgctga ttggtgacgg catcaaaact ggccttagta aaatgggttt
    31201 tagtgtcgac tggtttacac aaggtcgtca gggaaaagag gcgctttata gcgcacctta
    31261 tgatgcggtg atcctggatt taaccttacc aggcatggat ggtcgcgata ttttgcgcga
    31321 atggcgagag aaaggtcagc gtgagccggt actgaacctg accgcgcgtg atgcgttagc
    31381 ggaacgtgta gaagggctgc gtctgggagc tgacgattat ctgtgtaaac cttttgcgtt
    31441 gatagaagtc gccgccaggc tggaggctct gatgcgccga accaacggcc aggccagcaa
    31501 cgagctgcgc cacggtaacg tcatgctcga ccccggcaaa cgtatcgcca cgctggctgg
    31561 cgaaccctta acactgaaac caaaagaatt tgccctgctg gaattactga tgcgtaacgc
    31621 tggtcgggta ctgccgcgca aactgattga agagaaactg tatacctggg acgaagaggt
    31681 caccagtaat gccgttgaag tgcatgtgca tcatctgcga cgcaaactcg gtagtgattt
    31741 tattcgtacc gtgcatggta ttggctacac attaggtgag aaatgaaatt tacccaacgt
    31801 cttagtctgc gcgtcaggct gacgctaatc tttttaattc tggcctcggt gacctggctg
    31861 ctttccagct ttgtcgcctg gaaacaaaca acggataacg tcgatgaatt gttcgacacc
    31921 caactgatgc tgtttgccaa acggttaagt acgctcgatc tcaacgaaat caacgcggcg
    31981 gatcgcatgg cacagacgcc aaataaatta aaacacggtc atgttgatga cgatgcgctg
    32041 acctttgcca tctttaccca cgacggcaga atggtcctta atgatggcga taacggagaa
    32101 gatattccct atagctatca acgggaaggt tttgctgacg ggcaactggt cggtgaagac
    32161 gatccttggc gttttgtctg gatgacctca cctgatggca aatatcgcat cgttgttggc
    32221 caggaatggg aataccgtga agacatggcg ctggcgattg ttgccgggca attgatcccg
    32281 tggctggtcg cactgccgat tatgttaatc atcatgatgg tactactggg tcgtgaactc
    32341 gcgccgctga acaaactggc gctggcacta cgtatgcgtg accctgactc ggaaaaacca
    32401 ctaaacgcga ctggcgtacc cagcgaagtg cggccactgg ttgagtcgct aaatcaactg
    32461 ttcgcccgca cacatgcgat gatggttcga gaacgacgct ttacctccga tgcagctcac
    32521 gaactacgta gcccgttaac agcgctgaaa gtgcaaaccg aagttgcgca gctctctgac
    32581 gatgatccgc aggcgcggaa aaaagcactg ctccaattac attccgggat cgatcgcgct
    32641 actcgtctgg ttgatcaact gctcacgcta tcgcggctgg actcactgga taaccttcag
    32701 gacgtcgcgg agatcccgct tgaagatctc ctacaatcgt cggtgatgga tatttaccac
    32761 acggcgcagc aggcgaaaat tgacgtgcga ctgacactca atgcccacgg catcaaacgc
    32821 accggacaac cgctattgct aagtttgttg gtgcgaaatt tgctggataa cggccccggt
    32881 gtgacaccag aggcactggc gcgaattggc gaacgcttct atcgcccacc cggacaaacc
    32941 gctaccggca gtggacttgg gctatcgatt gtccagcgaa tcgccaagct gcatgacatg
    33001 aatgttgagt ttgggaatgc ggaacaaggt ggatttgagg cgaaggtaag ctggtaactt
    33061 ctctgtctat gagagccgtt aaaacgactc tcatagattt tactaatagc aaaatataaa
    33121 ccgtccccaa aaaagccacc aaccacaaac cagacaaata acgagcgaga atacatatag
    33181 ccaaaactcg aacatttccc gcataaagag ttttcttaag ataagaataa taagtggcgt
    33241 aagaagaaaa aatgctgcaa tcattgcttg caatatacga tcttcgcctg aaacggaagg
    33301 tatactgcaa atattgcctc ccgactcaag catccagtca tacttattga aagcaaaaaa
    33361 agaatctatt aaccaataag taagcattat atatggcaat gcttcaaata ttgttttaat
    33421 tttctgcttt aacatatatc agtacgctca taaaaatcca tttataatta tcaacatgac
    33481 acgttgatat tttcatcttt tacttattca aagccatatt ttcaatcccc aaaaaaaccg
    33541 aaccaaacgt gtaaattatt ccggtgcgag taagaattat gaggaatggc tatcagtatt
    33601 gtcattttca gaaaatattt atcctgcatc ggtgagtcag agctagatca gacttttgct
    33661 aaattcgcaa aagactttgc acattttgct aatttcacag taccgctctg tgacgtacta
    33721 tagtcgacaa acgtctcacc ttgagattaa aaaatgagca acatcctgat tatcaacggc
    33781 gcgaaaaaat tcgcccactc caatggtcaa ctgaacgaca ccctgaccga agtcgcggat
    33841 ggcacactgc gcgacctcgg gcatgatgtc cgcatcgttc gcgccgacag cgactacaat
    33901 gtcaaagcgg aagtacaaaa ctttctctgg gctgatgtgg tgatctggca gatgccaggc
    33961 tggtggatgg gcgcgccgtg gacagtgaaa aaatatattg atgatgtatt caccgaaggt
    34021 cacgggactc tgtatgccag cgatggtcgt acccgcaaag atccgtcgaa aaaatacggt
    34081 tccggcagcc tggtacagga caaaaaatat atgctttctc taacctggaa cgcaccaatg
    34141 gaagccttca ccgaaaaaga tcagttcttc cacggcgttg gcgttgacgg tgtgtatctg
    34201 ccgttccata aagcaaacca attcctcggt atggaaccgc tgccgacatt tatcgctaat
    34261 gacgtgataa aaatgcctga tgttccccgc tatactgaag aatatcgcaa gcatcttgtg
    34321 gaaatttttg gttaactaga gctcaggctt tagaaggagt taaccatgct taccgtaatc
    34381 gcagaaatcc gtactcgtcc tggtcaacat caccgtcagg cggtattgga tcagtttgct
    34441 aaaatcgttc caaccgtact gaaagaagaa ggttgccacg gctatgcgcc aatggtggat
    34501 tgcgctgctg gcgtgagttt ccagtctatg gcaccggatt ctatcgtgat gattgagcag
    34561 tgggaaagca tcgcgcacct tgaagcgcat ctgcaaaccc cgcacatgaa ggcgtacagc
    34621 gaagccgtaa aaggcgacgt gctggagatg aatatccgta ttctgcagcc agggatttaa
    34681 tcctgccttg tttgcccggc catcctgacc gggcaatgtt ctttccttta aacctcaatc
    34741 tccgccatgt cgcctttctc ttgcaaccag ttgcggcgat cttccgagcg tttcttcgcc
    34801 agcagcatat ccatcatcgc gtcagtacgc tgatcgtctt catcatcgat agtcaactgc
    34861 accagacggc gagtgttcgg atcaagcgtg gtttcacgca attgcatcgg gttcatttcc
    34921 cccagacctt taaaacgctg gacgttcggc ttgcctttct tgcgttttaa ttgctcaagt
    34981 acgccctctt tctcttcttc cgtcagcgcg taataaacct ctttcccgag atcaatacgg
    35041 tagagcggtg gcagtgcgac gtaaacgtga ccgtgtttca ccaacgcgcg gaagtgtttt
    35101 acgaacaaag cgcagagcag cgtggcaatg tgcagaccat cagagtccgc atccgcgagg
    35161 atacagattt tgccataacg aagctggctc agatcgtcgc tgtcaggatc gataccgatc
    35221 gctaccgaaa tatcatgcac ttcctgcgag gccagcactt cgtcggaaga aacttcccag
    35281 gtgttaagga tcttaccttt cagcggcatg atcgcctgat attcacgatc gcgcgcctgc
    35341 ttggcagatc cgcctgcgga gtcaccttcc acgaggaaca gttcggtacg gttaaggccc
    35401 tgcgcggtac aatcagccag tttgccaggc agcgccgggc cgctggtcag ctttttacgc
    35461 accacttttt tagccgcacg catacgacgc tgggcgctgg aaatcgccat ctctgccagc
    35521 aactccgccg cctgaacgtt ctggttcagc cacaggatga aggcatcttt caccacgccc
    35581 gaaacgaatg ccgcacactg acgcgaagag agacgctctt tggtctgccc ggcaaactgc
    35641 ggatcctgca ttttaaccga cagaacatag gcgcagcgat cccagatatc ttccgccgac
    35701 agcttcacgc cgcgcggcag aatgttgcgg tattcacaga actcacgcat cgcgtccaac
    35761 aggccctgac gcagaccatt aacatgggta ccgccctgca tcgttgggat aaggttgacg
    35821 tagctttcgg tcagcagttc accgccttcc ggcagccaca gtagcgccca gtccacagct
    35881 tcagtatcgc cagcgaaatt accgataaac ggtttttctg gcagcgtcgg taaaccgttt
    35941 accgcttccg ccaggtaatc attcagaccg tcctgatagc accagcgctg ttcggtgttg
    36001 ttgatctcat ctttaaaggt aatctcaacg ccggggcaca gtactgcttt ggccttcagc
    36061 acatgcgtca ggcgtgaaac agaaaaacgc gggctgtcaa agaaggtttc atccggccag
    36121 aagtgcacgc tggtaccggt attgcgttta ccgcaagtgc cgacaacctg taaatcctgc
    36181 accttttcgc cattttcaaa ggcgatgtta taaacctgac catcgcggcg cacgttaact
    36241 tctacgcgct tcgacaaggc gttaactacc gaaatcccca cgccatgcag gccgccagag
    36301 aactggtaat ttttgttaga gaatttaccg cctgcatgca gacggcaaag aatcagttca
    36361 accgccggta ctccttcttc cgggtgaata tccaccggca tcccgcgccc atcgtcaatc
    36421 acttctaacg actggtcagc atgtaagatt acatccacgc gttttgcgtg acccgccagt
    36481 gcttcatcga cactgttatc aataacttct tgccccaaat ggttagggcg agtggtatcg
    36541 gtatacatcc ccggacggcg gcgaaccggc tcaagcccgg tgagtacctc aatggcatca
    36601 gcgttataag tttgcgtcat ggtttaagtt agcaattcga gttgatcgtc agagatggtg
    36661 cagaccaaga aaatcgacga tcgggttgaa ataatcttcg aagcccgtga atgcgtggtt
    36721 gccgccttct atgacagtct ggcggcagga agcatagtac gccaccgcct ggcggtaatc
    36781 cagcacttca tctcccgtct gttgcagcag ccagatcaaa tccggcgctt ccagcgggtc
    36841 aatctgcatg actttgagat cgtaaatatg gcgtgactct agcacatatt gctgcccggt
    36901 gtaggggttc tcgttctgac cgagatagtc cgtcagcaat tcaaacgggc gtaccgccgg
    36961 gtttaccacc actgcgggca gcataaaaca ttgcgacaac caggtggcgt aatatccccc
    37021 cagtgacgaa ccgacaatac ccagcgaatc gccgccatgt tccaggacaa tggattccag
    37081 cagttctgcc gcgtcggaag gatacggcgg caactgcgga atgatcatct caacgtcagg
    37141 gtgatgttcc gccagccagt tttttaacaa gctcgctttt gcagagcgcg gcgagctgtt
    37201 gaaaccgtgt aaataaagaa gcgtagacat cagtagcctt ctgaagcggt atcaggttgg
    37261 aaacgtgtgt ccgccaggcg atgcacctcg gtggtcagcg tgccatcagc atgtaactcg
    37321 agagtacgcc agccgggcgc gatagtatcc agcgtaaagt tggaacagtg cggcttaaac
    37381 tgcacacagg tcgacggcgt tgccagcagg cggcgaccgt tccagtcgag atccagctcc
    37441 tgatgaatat gaccgcacag caagtatttg acgtgcggaa acttcgccag cacggtatcc
    37501 agttcgcccg cgttacgcag actgtgttga tcgagccaac tacaacccgc aggtagcgga
    37561 tgatgatgca gcagcagcaa cgtatggcgt tctggcgcat cggccagttt acgttccagc
    37621 cactcaagct gaaactcgct cagctcaccg tgcggcacgc caaacacctg gctatccagc
    37681 aacaggattt gccattgctc accaataaac acccgcttcg ccggggagat acccgcatcc
    37741 tgtaacgcgc tgtacatcgc gggctggaaa tcgtggttgc ccggcagcca gacgcagggc
    37801 gcacgaaaac ttgcgatgcc ttcagcgaaa tgctgatagg ccgcagagga ttgatcctgc
    37861 gctaaatcac ctgtcgcgac aatcaggtcg aattcgtgct ggtgtggacg aatcgcctcc
    37921 agcaccgcct ggtagctttc ccaggtgttt acccctaaca gggcttcgtg cttttgtgca
    37981 aacaggtgag tatcggtaat ttgtaaaatc cttcctctgg cctcaccagc cagaggaagg
    38041 gttaacaggc tttccaaatg gtgtccttag gtttcacgac gctagtaaac cggaatcgcc
    38101 atcgctccat gtgctaaaca gtatcgcaac cagtccgcta aaaactggtt aatttgatgc
    38161 ttttcgtcgc gttgatgcaa ctttttatta ggataatcat accgcgcttt gaagcgaaaa
    38221 atctgctggc ttgaacacac ttcagccacc atcgcgtcat gatacagacg caccgtcatt
    38281 gacggaaggc tccagtaact gatcgcgggc gcagtctgtt ctattgtcac cagggtagtg
    38341 tatcgggtcg attccacaat cgtcagccga tattgtgcgt ttgccacctg atagcttaca
    38401 gtttcgccgg gtgcgtcatt gcgcggtaac aaacggcgca attgtgaaaa gttcatctcg
    38461 cacaggcgca tcatttcagg aaagtcaggt gtgtaacgct tcatttatgc ccactcattt
    38521 tttaacgctt gatgatgcag ctgcagccat tgcaaagcga tgaccgacgc tgcgttgtcg
    38581 attttcccct cttctaccca ctggtatgcc tgttcccggc ttaccacatg aacgcgaata
    38641 tcttcgtttt catcagccag accatgaata ccgcttgcgg tcgtggcgtc cacttcgccc
    38701 accataattg acgaacgctc actggtgccc cccgggcttg ccaggaaact taacaccggt
    38761 ttggtccgtt tgactatcag tcccgcctct tcaatcgctt cgcgacgggc aacatcttcc
    38821 acactttcac cctcttcaat catcccggca accatctcca gtagccaggg ggtttcgctg
    38881 gtgtcgtacg cggcaatccg aatctgctca atcagcacaa cttcatcacg cactgggtca
    38941 aagggtagca agactgcggc gtgaccgcgc tcaaaaattt cccgccgtac ctcatgactc
    39001 atttgcccgt tgaatagacg atgacgaaat ctataaagat ctaatgaaaa aaagccgcga
    39061 taaagtgttt ctcgtgcaat aatttctaca tcgtttttgc caaatgtaac gggcaggttg
    39121 tctggcttaa gcattgttaa tgtcctagca ctaatagtga attaaatgtg aatttcagcg
    39181 acgtttgact gccgtttgag cagtcatatg ttaaattgag gcacattaac gccctatggc
    39241 acgtaacgcc aaccttttgc ggtagcggct tctgctagaa tccgcaataa ttttacagtt
    39301 tgatcgcgct aaatactgct tcaccacaag gaatgcaaat gaagaaattg ctccccattc
    39361 ttatcggcct gagcctttct gggttcagtt cgttgagcca ggccgagaac ctgatgcaag
    39421 tttatcagca agcacgcctt agtaacccgg aattgcgtaa gtctgccgcc gatcgtgatg
    39481 ctgcctttga aaaaattaat gaagcgcgca gtccattact gccacagcta ggtttaggtg
    39541 cagattacac ctatagcaac ggctaccgcg acgcgaacgg catcaactcg aacgcgacca
    39601 gtgcgtccct gcagttaact caatccattt ttgatatgtc gaaatggcgt gcgttaacgc
    39661 tgcaggaaaa agcagcaggg attcaggacg tcacatatca gaccgatcag caaaccttga
    39721 tcctcaacac cgcgaccgct tatttcaacg tgttgaatgc tattgacgtt ctttcctata
    39781 cacaggcaca aaaagaagcg atctaccgtc aattagatca aaccacccaa cgttttaacg
    39841 tgggcctggt agcgatcacc gacgtgcaga acgcccgcgc gcagtacgat accgtgctgg
    39901 cgaacgaagt gaccgcacgt aataaccttg ataacgcggt agagcagctg cgccagatca
    39961 ccggtaacta ctatccggaa ctggcggcgc tgaatgtcga aaactttaaa accgacaaac
    40021 cacagccggt taacgcgctg ctgaaagaag ccgaaaaacg caacctgtcg ctgttacagg
    40081 cacgcttgag ccaggacctg gagcgcgagc aaattcgcca ggcgcaggat ggtcacttac
    40141 cgactctgga tttaacggct tctaccggga tttctgacac ctcttatagc ggttcgaaaa
    40201 cccgtggtgc cgctggtacc cagtatgacg atagcaatat gggccagaac aaagttggcc
    40261 tgagcttctc gctgccgatt tatcagggcg gaatggttaa ctcgcaggtg aaacaggcac
    40321 agtacaactt tgttggtgcc agtgagcaac tggaaagcgc acatcgtagc gtcgtgcaaa
    40381 ccgtacgttc ctccttcaac aacattaatg cttctatcag tagtattaac gcctacaaac
    40441 aagccgtagt ttccgcgcaa agctcattag acgcgatgga agcgggctac tcggtcggta
    40501 cgcgtaccat tgttgatgtg ttggatgcga ccaccacgct gtacaacgcc aagcaagagc
    40561 tggcgaatgc gcgttataac tacctgatta atcagctgaa tattaagtca gccctgggta
    40621 cgttgaacga gcaggatttg ctggcactga acaatgcgct gagcaaaccg gtttccacta
    40681 atccggaaaa cgttgccccg caaacgccgg aacagaatgc tattgctgat ggttatgcgc
    40741 ctgatagccc ggcacccgtc gttcagcaaa catccgcacg cactaccacc agtaacggtc
    40801 ataacccttt ccgtaactga tgacgacgac ggggcttcgg ccccgtctga acgtaaggca
    40861 acgtaaagat acgggttatc tgccgcattc ttcccccttc tcgcttcaat ttcgaccagc
    40921 catcctctat tctgatgggt atttaccact ggtcccggaa gacaaaaatg aaacggacaa
    40981 aatccatacg ccacgcatcg ttccgcaaaa actggagcgc acgccatctg acaccagtcg
    41041 ctctcgcggt tgccactgtt tttatgctgg ctggctgtga aaagagtgat gaaacagtgt
    41101 ctctctatca aaatgctgac gactgttcag ctgcaaaccc aggcaaaagc gccgaatgta
    41161 ccaccgcgta caacaatgcg ctgaaagaag ccgaacgtac tgcgccgaaa tacgccaccc
    41221 gtgaagactg tgttgctgaa tttggtgaag gtcagtgtca gcaagcacca gcccaggctg
    41281 gcatggcacc agaaaaccag gcgcaggctc agcaatccag cgggagtttc tggatgccgc
    41341 tgatggccgg ttacatgatg gggcgtctga tgggcggcgg cgcgggattt gcacagcagc
    41401 cgctgttctc ctcgaaaaac ccggccagtc cggcttacgg taaatatacc gacgcgacgg
    41461 gtaaaaacta tggcgcagcc cagccaggcc gcaccatgac cgtaccgaag acggcaatgg
    41521 caccaaaacc ggcgaccacc actaccgtta cccgtggcgg ttttggtgaa tctgttgcca
    41581 aacaaagcac tatgcagcgt agcgcaaccg gcacctcttc tcgttcaatg ggtggctgat
    41641 accgatggaa agagtcagta ttaccgagcg cccggactgg cgtgagaaag cccacgaata
    41701 cggtttcaat tttcacacca tgtacggcga gccgtactgg tgtgaagatg cttactacaa
    41761 gttgaccctc gcccaggttg aaaagctgga agaagtcacc gccgaactgc accagatgtg
    41821 cctgaaagtg gtggaaaaag tgatcgccag cgatgagctg atgaccaaat tccgcatacc
    41881 aaaacacacc tggagttttg tgcgtcaatc atggctgact caccagccat cgctttattc
    41941 gcgtcttgat ctggcgtggg atggcactgg cgaacctaaa cttctggaaa ataacgccga
    42001 tacgccaacg tcactatacg aggcggcgtt ctttcagtgg atctggctgg aagatcagct
    42061 taacgccggt aacttgccgg agggcagcga ccagtttaac agtctgcaag aaaaactgat
    42121 cgatcgcttc gttgagctgc gtgaacagta tggcttccag ttgctgcatc tcacctgctg
    42181 tcgcgacacg gtggaagatc gcggaaccat tcagtatttg caggactgcg caaccgaagc
    42241 tgaaatcgcc accgagttcc tctacatcga cgagataggt ttaggcgaaa aaggccagtt
    42301 cacggattta caggatcagg tgatttctaa cctgttcaag ctttatccgt gggaatttat
    42361 gctgcgcgaa atgttctcca ccaagctgga agatgctggc gtacgctggc tggaaccggc
    42421 gtggaagagc attatctcca acaaggcact tctaccgcta ctgtgggaga tgttcccgaa
    42481 tcacccgaac ctgctgcccg cttattttgc ggaagatgat catccgcaaa tggaaaaata
    42541 tgtggttaaa ccgatcttct cccgtgaagg cgcaaacgtg tcgatcattg agaacggcaa
    42601 aaccattgaa gcagcggaag gtccgtatgg cgaagaaggg atgattgttc agcaattcca
    42661 cccgttaccg aaattcggcg acagctatat gctgattggt agctggctgg tgaacgatca
    42721 acctgccgga attggcattc gtgaagaccg tgcgttgatc acccaggata tgtctcggtt
    42781 ttatccacat atttttgttg aataagccac gataccggat ggcactcgcc atccggtaat
    42841 tgttagccta tctgcaccga cagcatactt aaactgccca tttctatacc atcgaccgga
    42901 atggtaattg gctcctgccc atcccacaca cctaacacat acaacaacgg cagatagtgc
    42961 tctggcgttg ggttcgataa cgcgccacct tcatggtcga gataattcac cagaggatgc
    43021 tgctctaccg gcccttgcca cgtcagattc gctttcacat actcattaaa cgacgtcgcc
    43081 cacggatacg gtgaactatc accgtgccac ttcactgtgc gcaggttatg caccacgtta
    43141 ccgctggcga ccaacattat tccttcatca cgcagcgccg cgagtttgcg ccccatttcg
    43201 aaatgccagg cggcaggttt gctactgtcg atactcaact gcaccatagg gatatcggcg
    43261 tcaggataca tcttaatcag cacgccccac gagccgtggt caaagcccca ggcttcttta
    43321 tccagcgtca ccgggatcgg cgctaacagc tcaaccagac gctgtgccag cgcaggcgaa
    43381 cccggagcag gataatgcgt atcgtacagc gcctgcggga agccaccaaa gtcatgaatc
    43441 gtgggcggcg tctccatcgc ggtcactcct gttccacggg taaaccagtg agccgaaacc
    43501 accacaatcg cttgcgggcg tggcaatgtc atccccaact tctgccagct gcgggtatac
    43561 aaattatctt ccagcacgtt catcggacta ccgtgaccta aaaacaatgc tggcatacgt
    43621 gttgaagaca tgatgatatc cttaactaaa ggtgtcattt tgatatcctc acaatacgct
    43681 tgttcggcgg agtaagaacc cggataacaa tgatgatgat catcagttat tttgacgatc
    43741 tgcctgaagg tgaagattta taaggagttg tcgatgtcag tacctctcat tctgaccata
    43801 ctggcggggg cagccacgtt tattggcgcg tttctcggcg ttctcggaca aaaaccctcg
    43861 aaccgcttac tggcgttttc gctaggtttt gcggcgggga tcatgttgct catctcatta
    43921 atggaaatgc ttcctgccgc actagcagct gaaggaatgt cgccggtgtt gggttatgga
    43981 atgtttatct tcggtctgct tggctacttt ggcctggacc gcatgttgcc gcatgcgcat
    44041 ccgcaggatt taatgcaaaa atcggtgcag ctgttgccaa aatcgatcaa gcgcacagcc
    44101 attctgctca ctctcggcat cagtctgcat aacttcccgg aagggattgc cacctttgtc
    44161 acggcgagca gcaacctgga gctgggattt ggcatcgcac tggccgtcgc gttgcacaat
    44221 atccctgaag gtctggcagt ggcaggcccg gtttatgcgg caacggggtc taaacgcacc
    44281 gcgattctgt gggcggggat ttctggacta gcagaaattc ttggtggtgt gctggcgtgg
    44341 ttaatcctcg gtagcatgat ttccccggtg gtcatggcgg caatcatggc ggcggttgca
    44401 ggaattatgg tggcgctctc ggttgatgaa ttaatgccgc tcgccaaaga gattgaccct
    44461 aataataacc ccagctatgg cgtactatgt ggaatgtcag tgatgggatt cagtttagtg
    44521 ctgctacaaa cggcgggaat tggttaaaaa caacatcaga agttgtccag cacatggaca
    44581 acttctgcca gcttcacgaa agccttattc taccttttcg gctgtctctt ctctcgtact
    44641 gtttaagtat ttgttccgcg cttcaagcag tgcgtctctg tcctggcgaa acgcccgaca
    44701 ataacgtttc aaatgcagga aataacccac cacaaccaga gcaatcaata caatccagta
    44761 ccaggcgata aacatccttt tacccttaat atttaacaat actaaacatt tagcgcataa
    44821 atttcacata tcctttttcg gatatattca tatggtcgca taataacgga acaattatat
    44881 gattttgatc ataatgaaaa agaatgcctg atattaatga aattttctca tagaaagaga
    44941 agcggaatta acatcttgca tagcagggca aaataaaaac cgaagccagc tacaacttcg
    45001 gtttcttgat taaggcagta attaagcagc ggttttcagc tggctttacg ctcatgtgcc
    45061 tgacggtatg ccaccaggtc ttcaatagtc acgagcgcca tattgtgttt attggcaaac
    45121 tcaatgcact ctggtgcacg cgccatcgtg ccatcgtcat tagtcagctc acacagcaca
    45181 ccagccggtt taaagcctgc cagcgtcatc aaatcaatag tcgcttcagt atgaccgcca
    45241 cgcgtcagta caccacctgc ctgcgcgcga agtgggaaaa cgtggcccgg gcgattcaga
    45301 tcagaaggtt ttgcgccatc ggcaatcgct gcgcgaacgg tcgtaatacg atcagcggca
    45361 gaaacaccgg tagtcacacc ttcagccgct tcaatggtca cggtaaaacc ggtgccatag
    45421 gcgctggtgt tattttctac catcattggc agatcaagtt gtttacggcg atcttcagtg
    45481 atgcacaggc aaacaatacc gctaccgtgg cgaatggtca gcgccatctg ctcaacggtc
    45541 atggtttctg ccgggaagat catatcgcct tcgttttcac ggtcttcatc atcaagcacc
    45601 attacaccgc gtccttcacg cagcgcagcc agtgcatttt caacacgttc gaaaggcgta
    45661 ccaaaagagg aaagtagcgt ctgattcatg gtaaaaaaac ctcactaaaa ttatggttac
    45721 cagaatcagg gcagtcttag gagtggcggc atatagccaa aataacgtga gcgggtccat
    45781 gcccgacaga atcgttactc tctcccatcc ggactctaac cgtcggcccc ggaattacac
    45841 cggatctgct gtcctttgag ttcgcaccca aagcgctcgc gggctttcaa ctgagttgat
    45901 ttaccgccgg tggggaattt cgccccgccc tgagaataag cggattcact ataacgctaa
    45961 tgattagcgg cagcaacgca tagcttcaca taattctggt ttatgactta cccttatcgc
    46021 actacaatgg tactcaacac ctatcagtac agggaaacta caatgattga cccgaaaaaa
    46081 attgagcaaa tcgctcgcca ggttcacgaa tcaatgccta aaggaatcag ggagttcggg
    46141 gaagatgtgg agaaaaaaat ccgccaaacc ctacaagctc agctgacgcg cctcgacctt
    46201 gtaagccgtg aagagttcga cgtccaaacg caagtcctgt tacggactcg tgagaaactg
    46261 gcgctgcttg aacaaagaat cagcgaactg gaaaatcgca gtactgagat caaaaagcag
    46321 ccagatccag agactctccc cccaacgctg taaacatttg tctttaatgc actgtaaatt
    46381 atgaattaat cctctaattt acagtgtttt ctattacatt atcgcttaat cccattcaga
    46441 gtatcatcac aaaaatgaat gattgcaatt gaaaatataa ttgttatgta ttctttaagt
    46501 tttacttaat cgata
//
LOCUS       DAZPCS010000017        46492 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZPCS010000017 DAZPCS010000000
VERSION     DAZPCS010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46492
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.017"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            334..459
                     /gene="timP"
                     /locus_tag="ACHUQ7_000908"
     CDS             334..459
                     /gene="timP"
                     /locus_tag="ACHUQ7_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFR1134432.1"
                     /translation="MKIRCFCIVLIVSGALFSQVNNNRSLSGDNLLVVNNLQSSK"
     gene            complement(491..1267)
                     /gene="sseB"
                     /locus_tag="ACHUQ7_000909"
     CDS             complement(491..1267)
                     /gene="sseB"
                     /locus_tag="ACHUQ7_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFR1134433.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(1409..2692)
                     /gene="pepB"
                     /locus_tag="ACHUQ7_000910"
     CDS             complement(1409..2692)
                     /gene="pepB"
                     /locus_tag="ACHUQ7_000910"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFR1134434.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAIMRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(2751..2951)
                     /gene="iscX"
                     /locus_tag="ACHUQ7_000911"
     CDS             complement(2751..2951)
                     /gene="iscX"
                     /locus_tag="ACHUQ7_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFR1134435.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(2963..3298)
                     /gene="fdx"
                     /locus_tag="ACHUQ7_000912"
     CDS             complement(2963..3298)
                     /gene="fdx"
                     /locus_tag="ACHUQ7_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFR1134436.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(3300..5150)
                     /gene="hscA"
                     /locus_tag="ACHUQ7_000913"
     CDS             complement(3300..5150)
                     /gene="hscA"
                     /locus_tag="ACHUQ7_000913"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFR1134437.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDATIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(5167..5682)
                     /gene="hscB"
                     /locus_tag="ACHUQ7_000914"
     CDS             complement(5167..5682)
                     /gene="hscB"
                     /locus_tag="ACHUQ7_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFR1134438.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSLAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(5778..6101)
                     /gene="iscA"
                     /locus_tag="ACHUQ7_000915"
     CDS             complement(5778..6101)
                     /gene="iscA"
                     /locus_tag="ACHUQ7_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFR1134439.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(6118..6504)
                     /gene="iscU"
                     /locus_tag="ACHUQ7_000916"
     CDS             complement(6118..6504)
                     /gene="iscU"
                     /locus_tag="ACHUQ7_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFR1134440.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(6532..7746)
                     /gene="iscS"
                     /locus_tag="ACHUQ7_000917"
     CDS             complement(6532..7746)
                     /gene="iscS"
                     /locus_tag="ACHUQ7_000917"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFR1134441.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(7858..8346)
                     /gene="iscR"
                     /locus_tag="ACHUQ7_000918"
     CDS             complement(7858..8346)
                     /gene="iscR"
                     /locus_tag="ACHUQ7_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFR1134442.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(8616..9356)
                     /gene="trmJ"
                     /locus_tag="ACHUQ7_000919"
     CDS             complement(8616..9356)
                     /gene="trmJ"
                     /locus_tag="ACHUQ7_000919"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFR1134443.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            9475..10278
                     /gene="suhB"
                     /locus_tag="ACHUQ7_000920"
     CDS             9475..10278
                     /gene="suhB"
                     /locus_tag="ACHUQ7_000920"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFR1134444.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKH"
     gene            10423..11277
                     /locus_tag="ACHUQ7_000921"
     CDS             10423..11277
                     /locus_tag="ACHUQ7_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR1134445.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQQLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            11468..12748
                     /gene="csiE"
                     /locus_tag="ACHUQ7_000922"
     CDS             11468..12748
                     /gene="csiE"
                     /locus_tag="ACHUQ7_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFR1134446.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(12740..13879)
                     /locus_tag="ACHUQ7_000923"
     CDS             complement(12740..13879)
                     /locus_tag="ACHUQ7_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFR1134447.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQVSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(14039..14929)
                     /gene="hcaR"
                     /locus_tag="ACHUQ7_000924"
     CDS             complement(14039..14929)
                     /gene="hcaR"
                     /locus_tag="ACHUQ7_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFR1134448.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KLALRDFIAIVQERLASVTA"
     gene            15065..16426
                     /gene="hcaE"
                     /locus_tag="ACHUQ7_000925"
     CDS             15065..16426
                     /gene="hcaE"
                     /locus_tag="ACHUQ7_000925"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFR1134449.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNSRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            16423..16941
                     /gene="hcaF"
                     /locus_tag="ACHUQ7_000926"
     CDS             16423..16941
                     /gene="hcaF"
                     /locus_tag="ACHUQ7_000926"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFR1134450.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            16941..17261
                     /locus_tag="ACHUQ7_000927"
     CDS             16941..17261
                     /locus_tag="ACHUQ7_000927"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFR1134451.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            17258..18070
                     /gene="hcaB"
                     /locus_tag="ACHUQ7_000928"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             17258..18070
                     /gene="hcaB"
                     /locus_tag="ACHUQ7_000928"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFR1134452.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTTSK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            18080..19297
                     /gene="hcaD"
                     /locus_tag="ACHUQ7_000929"
     CDS             18080..19297
                     /gene="hcaD"
                     /locus_tag="ACHUQ7_000929"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HFR1134453.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLHHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRSAAQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVV
                     DGEKVELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIF
                     AGGDVAITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQ
                     FIGDMRGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDA
                     KLLIDENIALKSL"
     gene            19394..19816
                     /locus_tag="ACHUQ7_000930"
     CDS             19394..19816
                     /locus_tag="ACHUQ7_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFR1134454.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(19864..20736)
                     /locus_tag="ACHUQ7_000931"
     CDS             complement(19864..20736)
                     /locus_tag="ACHUQ7_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFR1134455.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDEHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(20748..21842)
                     /locus_tag="ACHUQ7_000932"
     CDS             complement(20748..21842)
                     /locus_tag="ACHUQ7_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFR1134456.1"
                     /translation="MKINRIVNEGFMKTMLAAYLLGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGGMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYVLMASGKCGKVVINFPD"
     gene            complement(21875..22873)
                     /locus_tag="ACHUQ7_000933"
     CDS             complement(21875..22873)
                     /locus_tag="ACHUQ7_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1134457.1"
                     /translation="MYASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(22898..24409)
                     /locus_tag="ACHUQ7_000934"
     CDS             complement(22898..24409)
                     /locus_tag="ACHUQ7_000934"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFR1134458.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASY
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(24432..25414)
                     /locus_tag="ACHUQ7_000935"
                     /pseudo
     CDS             complement(24432..25414)
                     /locus_tag="ACHUQ7_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
     gene            complement(25511..28792)
                     /locus_tag="ACHUQ7_000936"
                     /pseudo
     CDS             complement(25511..28792)
                     /locus_tag="ACHUQ7_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
     gene            28910..30103
                     /locus_tag="ACHUQ7_000937"
     CDS             28910..30103
                     /locus_tag="ACHUQ7_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFR1134459.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGSLETVASLSALKKQARVWLK
                     SQPVNTQLEPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(30301..31554)
                     /gene="glyA"
                     /locus_tag="ACHUQ7_000938"
     CDS             complement(30301..31554)
                     /gene="glyA"
                     /locus_tag="ACHUQ7_000938"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFR1134460.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            31881..33071
                     /gene="hmpA"
                     /locus_tag="ACHUQ7_000939"
     CDS             31881..33071
                     /gene="hmpA"
                     /locus_tag="ACHUQ7_000939"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFR1134461.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(33116..33454)
                     /gene="glnB"
                     /locus_tag="ACHUQ7_000940"
     CDS             complement(33116..33454)
                     /gene="glnB"
                     /locus_tag="ACHUQ7_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFR1134462.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(33515..34849)
                     /gene="glrR"
                     /locus_tag="ACHUQ7_000941"
     CDS             complement(33515..34849)
                     /gene="glrR"
                     /locus_tag="ACHUQ7_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFR1134463.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(34839..35552)
                     /gene="qseG"
                     /locus_tag="ACHUQ7_000942"
     CDS             complement(34839..35552)
                     /gene="qseG"
                     /locus_tag="ACHUQ7_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFR1134464.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(35717..37144)
                     /gene="qseE"
                     /locus_tag="ACHUQ7_000943"
     CDS             complement(35717..37144)
                     /gene="qseE"
                     /locus_tag="ACHUQ7_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFR1134465.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(37702..41589)
                     /gene="purL"
                     /locus_tag="ACHUQ7_000944"
                     /gene_synonym="purI"
     CDS             complement(37702..41589)
                     /gene="purL"
                     /locus_tag="ACHUQ7_000944"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFR1134466.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVVQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFQRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            41848..43404
                     /gene="mltF"
                     /locus_tag="ACHUQ7_000945"
     CDS             41848..43404
                     /gene="mltF"
                     /locus_tag="ACHUQ7_000945"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFR1134467.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(43401..43904)
                     /gene="tadA"
                     /locus_tag="ACHUQ7_000946"
     CDS             complement(43401..43904)
                     /gene="tadA"
                     /locus_tag="ACHUQ7_000946"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFR1134468.1"
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(43962..44597)
                     /gene="yfhb"
                     /locus_tag="ACHUQ7_000947"
     CDS             complement(43962..44597)
                     /gene="yfhb"
                     /locus_tag="ACHUQ7_000947"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFR1134469.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            44806..45654
                     /locus_tag="ACHUQ7_000948"
     CDS             44806..45654
                     /locus_tag="ACHUQ7_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFR1134470.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            complement(45993..46492)
                     /locus_tag="ACHUQ7_000949"
                     /pseudo
     CDS             complement(45993..46492)
                     /locus_tag="ACHUQ7_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT        11055 a        12790 c        11692 g        10955 t
ORIGIN      
        1 tcctttaaaa aacaaaaaac cgccaaaatc aggcggttat ttgttgctgg tccggttcgc
       61 ggcctttcca gcaggttgta ttaccgtagt aatgcaagcg cgtctcagcg gagacaatac
      121 tcgccagtaa ctctcttttt gtcaagcaaa agagagttat tattgttctg ttagtgtatt
      181 atccactgcg gccctttccg ccgtctcgca agcgggcgct ggcttaagga aaggatgttc
      241 cgcgaccgtt agagcaagcg cgtctcagcg cttgctatcg cggcaatatc gccagtggtg
      301 ctgtcgtgat gcggtcttcg catggaccgc acaatgaaga tacggtgctt ctgtatcgta
      361 cttattgttt ctggtgcgct gttttcccaa gtaaataata accggagtct ctccggcgac
      421 aatttactgg tggttaacaa ccttcagagc agcaagtaag cccgaatgcc gccctttagg
      481 gcggcatatt ttagattatc cggttttgtt taaagtcacg caaaaaacca ccccagcgac
      541 gttcatagaa tggcgcaata tgttcggtaa taaagtggct aattcctttt tccccttttt
      601 tcacctgaca aatatcgatt ggttcatcgc caggtaatgt atcggtcgct acacttcccg
      661 tcgcctgaat aatttcttcg atatcaccat cggcttcaat gccaataagt aaattaggct
      721 gtgcctcttc gttctcttta attgaacaaa taaaagcacg cttcaccggc ttaatggttt
      781 taaataaggt ggtgagtgaa tcaatcattt gtgctggcgg ctctgcgact tccgataata
      841 tcagcgattc accgccttcc aggatttcct ggctgctcag cggatttccc tcttcaccaa
      901 tcaacaaact gatttcacgc ggcataaatt ctttaccggt cggcagtttg gcattaagga
      961 agagcgtttc gccaagggtc atctcaaaca atgtgcgaac gggcattacg acaaatgcct
     1021 gttcgtcttc aaccgcctgt tgaagcgctt ctaacgaggt gaaaaaagga atgacgctgg
     1081 tgccgtcttc tttttcccag tgctgtaaat caagcgcact atcttcaacc acagcctcgc
     1141 cctgcgccgc cgtaccaggc acccagacgg tggattccag tagagtacgg aaaaaggccg
     1201 ggcggtgcgc cggttcggtt gctgcttttt ccagcaggtc ttcaagttcg ttttttgttt
     1261 cggacataag aaccacaatt cattcaacgt tcggggcgca aatgcctgat gcgctacgct
     1321 tatcaggcct acaagggatt cgcaatttgt tgaatttgca gaatttgtag gccggataag
     1381 gcgtttacgc cgcatccggc atattagttt acgccgttaa cagattagct atcgtgcgca
     1441 caccaagtcc cgtagcgccc gcagaccact gttcaaccgg cgctttacgg taagtcgccg
     1501 agcagtcgat atgcagccag ccttgctgat agttctcaac aaagtgcgac aggaagcccg
     1561 ccgccgtgct cgcgcctgcc ggatacgccg cgcttccggt attgttcagt tcggcaaagt
     1621 tagacggcag ctggctgcgg tggaattccg ccagcggcag acgccagaat ggttcgttct
     1681 cttgtgaggc actcgccagt aaacgaccgg caagcgcatc gtcaaaactg aacagcgcgt
     1741 gataatcatt acccagcgca gttttcgccg ccccggtgag ggtcgccgca tcaatgatca
     1801 tttccggttt ctgcgcactg gcatcaatca gaccatcggc aagcaccaga cgcccttcgg
     1861 catcagtgtt catcacttca acttttttac cgttgcgata ggtgatgata tcgcccagct
     1921 tgaacgcatt gccgctaatc aggttatccg cacagcagag gaacagcttc acgcgcttgt
     1981 tcagtccgcg cataatggca aatgccagcg ccccggtaac cgttgccgcg ccgcccatgt
     2041 ccgacttcat cgagtccata aacgcagtct gtttgatgct gtagccgccg gagtcaaaag
     2101 tgataccttt acctaccagg cacgcgtaca ccggtgcttc tttatcgcca gttgggttgt
     2161 aatccagcgc cagcaatacc ggagaacgtt ctgaaccgcg cccgacagtg tgcagcccca
     2221 tataaccttg ctcacgcaga tcttcgcctt tggtgatccg ataagtcaca cgatcgcccg
     2281 cgacgttgct gatcagatca acagcacgct gtgccagttg cgatggtccc aattcttctg
     2341 ccggtgcgtt gatggtgtca cgcacccagt cgatgatcat caggcggtta tccagttcct
     2401 ggcgctgggc atcgtccaga tccggccact ccactttacg cgtgcctttc gggcctttat
     2461 aaccttgcca gaatgcccag cagcgatccg catcccagcc ttcaccgctt aactgaacat
     2521 gcttgatgcc cagaccgtca atcttgcggg ccgcacgctg gatcagcccc agatcgtctg
     2581 ccccgttcaa atgcagggta atgccgtcat tattaatgct gtaagttgct ttttctcccc
     2641 agcgcgcgtc ggcaggttgg gtagagaggg taatcttcat cgcttctgtc attttagtta
     2701 tccttattcg tttaacagca aaacgggcta cccccaggca gcccgttcat tcactcggct
     2761 tcgtccagcc agactaacaa aatcgcttcg aggattttct cgttggatgc ctgcgggtcg
     2821 tcgtcgaaat cttccagatc gcaaatccac tgatgcatat cggtgaatcg aaccgttttc
     2881 ggatcaagat cgggatacgc atcgtacagt gcttcgccaa tttcgcggct atcggtccac
     2941 ttaagtccca tactaacctc tgttaatgct cacgcgcatg gttgatagtg taacgcggga
     3001 tttcgaccac taaatcttcg tcggtgacgc gcgcctggca gcttaaacgg ctttccggct
     3061 ccagtcccca ggctttgtcc agcatgtcgt cttcctgctc tgagctttcc ggcagtgagt
     3121 caaaaccttc acgaacgatg cagtggcagg tggtgcaagc acaggatttt tcacaggcgt
     3181 gttcaatctc aataccgtta cgcagcgctg cgtcgagaat ggtttcaccg ctattagctt
     3241 ccagaacagc gccatcaggg cagaggtcct gatgaggcaa aataacaatc tttggcatat
     3301 taaacctcgt ccacggaatg gcctttcagc gcacgacgaa ccgactggtc catgcggcga
     3361 gcggcgaaat cctgggtttg tttgtctacg tttttaatcg cttgttcgat ggcgtcaaca
     3421 tcatcgccct gcgccacttc actcaggtga gcggcagcat tgtcaatgac ctgacgctct
     3481 gcggcgctta acagcgcggc atcagcagcc agcgcgccgt gcagactttc cagcacacgc
     3541 gccgcttcta ctttttgttc tgccagcata cgggctttta cgtcctgctc ggcatagctc
     3601 attgagtctt tgatcatcga agcgatttcg ctatcggtca gaccgtaaga cggtttgacc
     3661 tgaatagacg cctcaacgcc ggtggatttc tccatcgccg tcacgctcaa aagaccgtcg
     3721 gcatcgacct ggaacgtcac gcgaatatgc gcaccgccag ccggtagcgc cggaatacca
     3781 cgcagcgcaa aacgcgccag tgagcggcag tcctgcacca gttcgcgctc accctgcatt
     3841 acatggatag acatcgccgt ctgaccatct ttaaaggtgg tgaaatcctg agcgcgggcc
     3901 accggaatag tggtattacg cggaatcact ttctccacca ggccgcccat cgtttcgagg
     3961 cccagcgaca gtgggatcac atcaagcagc agcatttcgc tgtctggctt gttacccacc
     4021 agaatatccg cctgaatcgc cgcgccaata gcgacgactt tatccgggtc gatggaagtc
     4081 agcggtggac gaccgaaaaa ttcgcctacc cgttcacgca ccagcggcac gcgagtagaa
     4141 ccgcccacca tcaccacttc cagcacttcg tcagcttcta cacccgcgtc tttcagcgcg
     4201 cgacgacaag ccagtaaggt tcgttttacc agtggcgcga tcagttcatt gaattgttca
     4261 cggctgattt cgccctgcca gcccgcaacg ttaacggtca cggagtccgc atcgctcagc
     4321 gcgattttgg ctgcaatggt ggcatccagc agttcacgct gaacgcggtt atcgctacga
     4381 tcaggaatgt ccgcctgctc gcgaatgtaa tccgccagca gatggtcgaa atcatcgccg
     4441 ccgagcgcgg aatcaccgcc ggttgccagc acttcaaaca cgccgcgact taagcgcaga
     4501 atggaaatat caaacgtccc gccaccgagg tcataaacag cgatcacgcc ttcctgaccg
     4561 gaatccagcc cgtaggcgat agccgcagca gtcggttcgt taagtaagcg cagaacatgc
     4621 aaccccgcca gacgcgccgc gtctttggtg ccctgacgct gggcatcgtc aaagtacgcc
     4681 ggaacggtga taactacacc atccagctcg cctgccaggg cttcagttgc ccgcgccgcc
     4741 agtgctttga ggatgtccgc agaaacgcgc accgggttca gtagccccgc cgccgtttca
     4801 atcatcggca ggccgttttc gctggcctgg aattgataag gcagatgcgg atagcgttgc
     4861 tggatatcag ccagcgagcg ccccatcagg cgtttaacag aactaatggt gttggcggtg
     4921 tcaagcgctg cgttagtacg cgcgtcataa cccaccgaat gcccttgctg ttgatagtga
     4981 acaacagatg gcagcaggtg acggccttca tggtcggcta acgtttcggc ctgaccgctg
     5041 cgcactgtcg ccaccagcga gttggttgtg cccaggtcaa taccagccgc cagacgacgc
     5101 tgatgcggcg cagcactcaa accaggttca ctaatttgta ataaggccat gtttagcttc
     5161 cagaaattaa aaatcgagca gtttttcttc gagttgttcg gcactgctcc gcagtttatc
     5221 gagaaaacgc agcttacgca ccgtatccgc cgccgcgtcc cacgcctcgt tgtctaactg
     5281 ttcaaccatc aactgatggc gggtatcaaa catctttttc acacgtttga taaagctttc
     5341 cagccgcgct tcatctttcg cctgttcgat ctcgtccagc tcttcgcgca gctccaactg
     5401 ttccatcagg aacgcggtgt cgcgcacagt atgctgctcg ctggcgagat caaaaccgtg
     5461 taaagaaagc aaatattccg cgcgcattaa cggatgacgc agcgtttgcc aggcctggtt
     5521 aatggttgca gattgctgta cggcggcgag ttgttccgcc aggcttccgc tggcgaattt
     5581 atcaggatga tactgacgtt gtagatcctg aaaacgcagg ctcagtgcct gggtatcgag
     5641 ttgataacgg gcaggcaagc caaagagggt gaagtaatcc ataacattct cagggctgcg
     5701 atccgccgcg ataacggcga atcaggtaaa acaaacccca cgcgcaggcg accacggtgg
     5761 ggttatcggt atgcgcatca aacgtggaag ctttcgccgc aaccacactc atctttaacg
     5821 ttcgggttgg tgaatttaaa cccttcattc aggccttctt ttacgaagtc cagctgcgta
     5881 ccgtccagaa attgcaggct tttgccatcg accacgactt tcacgccttt gtcttcaaac
     5941 acgatgtctt ccggcgtcgg ttcgtcaaca aattccagta cataagccat acctgaacat
     6001 ccggaggttc tcacgcccag acgcaggcca aaccctttgc cgcggttagc cagaaaggta
     6061 tttactcgcg ctgctgcact gtcgctcagt gtaatcgaca taaccaaacc tcaactctta
     6121 ttttgcttca cgtttgcttt tatagtccgc aatggcggct ttgatcgcgt cttctgccag
     6181 aatagaacag tgaattttca ccggcggcag ttcaagttct tcagcaatat cggtgttttt
     6241 gatcgcctgc gcttcgtcga gagacttccc tttcacccat tcggtgacca gggagctgga
     6301 agcgatagcg gaaccgcagc cgtaagtttt aaaacgcgcg tcttcaatga taccttcatc
     6361 gttgacttta atctgcaact tcattacgtc gccacaggcc ggtgcaccca ccatgccgct
     6421 gccgacgttc tcgtcgttgt tatcaaagga acccacgtta cgcggattct cgtaatggtc
     6481 gataactttt tcgctgtaag ccattataaa ttctcctgat tccgataccg attaatgatg
     6541 agcccattcg atgctgttca gatccacgcc ctgcttgtac atttcccaca gcggagaaag
     6601 gtcacgcaga cgaccgatgg atttacgaac taactcgatg gtgtagtcga tctcttcttc
     6661 agtagtaaaa cgacctaaag agaaacggat agagctatgt gccagctcgt cgttcagccc
     6721 cagcgcgcgc agcacgtagg acggttcgag gcttgctgac gtacaggcgg aacctgaaga
     6781 aactgcgagg tctttcagcg ccataatcag cgactcacct tcaacgtagt tgaagctgac
     6841 gttgagaatg ttcggcgcac cgtgttccag gtcaccgttc aggtaaactt cttcgatatc
     6901 tttgatgccg ttccacagac ggttacgcag gccgcgcaga cgttccatct cggtcgccat
     6961 ctcttctttt gcgatgcgat aggcttcgcc cattccgacg atctggtgaa caggcagagt
     7021 gccggaacgc ataccgcgct cgtgaccgcc gccgtgcatt tgcgcttcga tgcgtacacg
     7081 cggtttacga cgcacataca gcgcaccgat acctttcggg ccatagattt tgtgaccgga
     7141 gaaagacatc aggtcaactt tcaactggct caggtcgatt ggcagtttgc ccacgctctg
     7201 ggttgcatca acgtgataga taatgccacg agcacggcac atttcgccga tagccgcgat
     7261 atcctgcacc acgccgattt cgttatttac gtgcatgatg gaaacaagga tggtgtcgtc
     7321 acgcatcgcc gcttcgagtt ctttcaggtc gataataccg ttacgctgcg gtgccaggta
     7381 ggtgacttcg aaaccttcgc gctccagctg acggcaagta tccagtaccg ctttgtgttc
     7441 ggttttgctg gtgatgatgt gcttgccttt tttctgataa aagttggctg cacctttgat
     7501 cgccaggttg tcagattcgg ttgcaccaga ggtaaagacg atttcacgcg gatcagcgcc
     7561 gaccagatcg gcaatctgat tacgggcgat atctaccgct tcttcagcct gccagccgaa
     7621 acggtgagaa cgagaggccg ggttaccaaa ggttccgtcc atcgtcatga actgcatcat
     7681 tttctcggca acacgcgggt ccaccggcgt ggttgcggag tagtcgagat aaatcggtaa
     7741 tttcattgct ctataaactc cgtacatcac tcaatgcaag gaatcaggct accggctgga
     7801 tgtacgaccg tgtttacgga gtatttagca ctccggcctg attctgaatt ctttttatta
     7861 agcgcgtaac ttaacgtcga tcgcgtcttg tgtgcgggtg cgtggcgcgt cgtgagtatg
     7921 ctgacgacca gacacatcca gcacttcctg gttattaacc agttcgccta aagtaatgtt
     7981 gttgagaaaa ccggtgagac ggtcgctcaa atcacgccac agcgcgtggg tcaggcattt
     8041 atcgccgccc tggcagccgc ctttaccctg acaacgggtg gcatctacag attcgtcaac
     8101 ggcgctaatc acttcgccaa cggcgatgct gctggcatct ttgcctaaca gataaccacc
     8161 gcctggtcca cgtacgctgg aaaccagacc atttttacgc agacgggaaa acagttgttc
     8221 cagataagaa agggaaattc cctgacgttc ggaaatatca gccaacggta ccgggcccgc
     8281 ttcagagttg agcgcaacgt caagcattgc ggtcacggca tagcgccctt tagatgtcag
     8341 tctcatgtct tacttcacct caaactcgcc cctgcccggg gttttttatt gtaaaagtgg
     8401 gggtattgca tagcagggtc aagtctgaca ttcccgagta aattggtcaa ctatttactt
     8461 gactgattta gtcgggtatt taaccttcag tgccattttt ttatcgtggc gtttgtacct
     8521 gttgtcggat gcggtgtgaa caccttatcc gacctacgat tctgtacatt gtaggcctga
     8581 taagacgcgt taagcgtcgc atcaggcaac cactgttatt cggccttgtt acctttattc
     8641 tgctgctcaa tagaagccag aatcccgcgc aggatattca attcctggct ttccgggcgc
     8701 gcacgggtaa acagacggcg caatttattc atcacctgcc ccggatggtt ttcacggata
     8761 aaaccggttg ccagcagcgt ttgttccaga tgaccataaa aacgctccag atcatcgacc
     8821 agcggatacg gcgtctcttc atgctcgacc tgctcgccat tttcctgagt tgccagccag
     8881 gccatacgca cttcataggc aataacctga accgccattg ccaggttcag cgaactgtat
     8941 tccgggtttg ccgcaatcgc gacatgataa tggcatttct gcaactcttc attggtcaag
     9001 ccgacgcgct cacgaccaaa caccagcgcc accggggtat ttgccgcttc agcgacgctt
     9061 ttcagaccgc attcgcgcgg gtcgagcatc ggccacggca gcgtgcggga gcgtgcgctg
     9121 gtgcccacca ccaggctaca accggctaac gcttcgtcga gcgtatcgac gatgtgagca
     9181 ttaccaatca catcgctggc ccctgcagcc agggcaatcg cctgggagtc gggtttcacc
     9241 agtggattaa ccagccacag attggttaat cccattgttt tcatggcacg ggcaacagaa
     9301 cccatattgc cggtgtgtga cgtctccacc agcacaattc gaatattttg cagcattgtc
     9361 tttcttcatc taaagattat tcacgcatct tatcataaaa cgaagacaga tgccgatctc
     9421 gctgctatac tctgcgccgt tttcccgttc tttaacatcc agtgagagag accgatgcat
     9481 ccgatgctga acatcgccgt gcgcgcagcg cgcaaggcgg gtaatttaat tgccaaaaac
     9541 tatgaaaccc cggacgctgt agaagcgagc cagaaaggca gtaacgattt cgtgaccaac
     9601 gtagataaag ctgccgaagc ggtgattatc gacacgattc gtaaatctta cccacagcac
     9661 accatcatca ccgaagaaag cggtgaactt gaaggtactg atcaggatgt tcaatgggtt
     9721 atcgatccac tggatggtac taccaacttt atcaaacgtc tgccgcactt cgcggtatct
     9781 atcgccgttc gtatcaaagg ccgcaccgaa gttgctgtgg tatacgatcc tatgcgtaac
     9841 gaactgttca ccgccactcg cggtcaaggc gcacagctga acggctaccg tctgcgcggt
     9901 agcaccgctc gcgatctcga cggtaccatt ctggcgaccg gcttcccgtt caaagcaaaa
     9961 cagtacgcca ctacctacat caacatcgtc ggcaagctgt tcaacgaatg tgcagacttc
    10021 cgtcgtaccg gttctgcggc gctggatctg gcttacgtcg ctgcgggtcg tgttgacggt
    10081 ttctttgaaa tcggtctgcg tccgtgggat ttcgcggcag gcgagctgct ggttcgtgaa
    10141 gcgggcggca tcgtcagcga cttcaccggt ggtcataact atatgctgac cggtaacatc
    10201 gttgctggta acccgcgtgt tgtgaaagcc atactggcga acatgcgtga cgagttaagc
    10261 gacgctctga agcattaatg actcaggcgg gtgatatcac tcacccgccc tcgcctttca
    10321 ggcgctattc cgaaatactt cctcaccgct ttactttctt tcgtcactct cccaccatct
    10381 ttccccgatt aatggatgat aaagaagtcg caaggaaagg ttatggcact gccagtgaac
    10441 aaacgcgttc ccaaaattct gtttattctc tttgttgttg ccttctgcgt ttatttagtg
    10501 ccgcgcgttg ccatcaactt cttctattat cctgacgata aaatttacgg tcccgacccc
    10561 tggtcggcgg aatccgtcga atttacggct aaggacggta ctcgtctgca aggctggttt
    10621 attccttctt cgacgggccc tgctgacaac gccatcgcaa ccatcattca tgctcacggc
    10681 aatgccggaa atatgtccgc ccactggccg ctggtcagtt ggttacctga gcgtaatttc
    10741 aacgttttta tgtttgatta tcgcgggttt ggtaaatcaa aaggcactcc gtcccaggcc
    10801 ggattgctgg acgatacgca aagcgccatc aatgtggtgc gccatcgcag tgatgtaaac
    10861 ccacaacagc tggtgctgtt cgggcagagt attggcgggg cgaatattct tgctgttatt
    10921 ggtcagggtg atcgtgaagg catacgtgcg gtgatcctcg actccacatt tgcctcttat
    10981 gcaaccatcg ccaaccagat gatccccggc agtggctact tacttgatga gagttacagc
    11041 ggcgaaaatt acatcgccag cgtcagcccg atcccgcttt tactcattca cggtaaagcc
    11101 gatcacgtta tcccatggca gcacagcgaa aagttgtata gcctggcaaa agagccaaaa
    11161 cggttgatcc taatcccgga tggcgaacac attgatgctt tttccgatcg tcacggcaat
    11221 gtttatcgcg aacagatggt ggactttatc cttagcgcgt tgaatccgca gaactaaccc
    11281 atgatcgcta gcacgataat cattcacaaa accaccttaa gacatgctaa tccactggtc
    11341 agaacagttt aaaatgagaa aaattctgtg acgcttgcca acatttctga tgattagcat
    11401 tcccttcgcc atttccctga gcaaacttta gctattctta tcaattatgc ttatgggaga
    11461 tctacagatg atgcctacgc ttgctccacc atctgtcctt tcggctcccc agcgccgctg
    11521 ccagatcttg ctgacgctct ttcagccggg gttgaccgcc accatggcaa ccttcagcga
    11581 gcttaatggt gtggatgatg atattgccag tcttgatatc agcgaaacag gacgggagat
    11641 cctgcgctat catcaactca cactgacgac tggttatgac ggtagctacc gggttgaagg
    11701 tacagtgctt aaccaacgtt tgtgtttatt tcactggcta cgacgtggtt tccgtctgtg
    11761 tccgtcattt attaccagcc aattcacccc cgccctgaag agtgaactga agcggcgcgg
    11821 aattgcgcgt aacttttacg acgataccaa tctacaagcg ttagtgaatc tctgctcccg
    11881 acggctgcaa aaacgctttg aatcgcgcga tattcatttc ctgtgtctgt atctgcaata
    11941 ttgtttgctg cagcaccacg ctggaattac gcctcagttt aatccgctcc aacgtcgctg
    12001 ggcggaatcc tgccttgaat ttcaggtagc gcaggaaatt ggacgccact ggcagcgtcg
    12061 ggcgctccag catgtaccgc ctgatgagcc actgtttatg gcattacttt tttccatgtt
    12121 gcgggttccc gatccactgc gggatgcgca tcagcgggac agacaattgc gtcagtctat
    12181 caaacgtctg gtaaaccatt ttcgtgagct gggaaatgtt cgtttttatg atgaacaggg
    12241 gttatgtgat cagctttata tccacctcgc ccaggcgtta aatcgcagtt tgtttgccat
    12301 cggtattgat aataccctgc cggaagagtt cgccagactg tatccacgcc tggtacgcac
    12361 cacccgcgcg gcgctggccg gatttgaaag tgaatacggc gtccatcttt ctgatgagga
    12421 aagtggtctg gtcgcggtga ttttcggtgc ctggctaatg caggaaaacg atctgcatga
    12481 aaaacagatt attctactga ccgggaatga tagcgagcga gaagcgcaga ttgagcagca
    12541 gttacgcgaa ctaacgttac tgccgctcaa cattaagcat atgtcggtaa aggcattttt
    12601 gcagacaggc gctccgcgtg gcgcggcact gattattgcg ccttatacca tgccgttacc
    12661 gctcttttca ccaccgctga tctatacgga cctgacgctg acaacacatc aacaggagca
    12721 gatccgcaaa atgctcgaat cagcatgagg gaacaacttt cgggcgcaaa aacattgccg
    12781 gaagcgccac cagcgccatt acccagaaca cgccgtggcc cagatattga tacaggaaac
    12841 cggcgaaaac ggtcatgata gcgatactgc cgcccatcgc gacggcagag taaaccgcct
    12901 gtaaacggat gacttcgcta acctggcgag cagcaatata acgcatggcg gccaggtggc
    12961 agaccgtgaa ggtgccgcaa tgcagaattt gcaccactat caaccacggc aacgccgtag
    13021 ttgctcccat aatgccccag cgcactacgc cgcaaatcgc cgagatcaac agcatatcgc
    13081 gagcactaca acggcggaaa agtttattac tcagcgcaaa gataatgact tccgccacca
    13141 cgcccagcga ccacaaatac cccaccgccg aggccgagta gccagctgcc tgccagtaaa
    13201 tggcgctaaa accgtaatag gccgcatgtg ccccctgcaa taaacaaacg caggccagaa
    13261 agcgccagtt ctggcgaacc agcgccaacc acgcagacca accggtgctc tcctgctggc
    13321 ggcttgcccc ttgtggctga atcgtcggac ggatgagaaa gccgagcagc atggatgcca
    13381 cgcccaacgt caacagcacg aggatcaccc gataatcaaa catagtgacc agtttgcccg
    13441 tcagcgccga gccaatgaca aacgccaccg agccccacag tcgcactttg ccgtaatcaa
    13501 gcgggaactg cttttgccac gtattcgcca gtgcatcggt caacggtacc agcggtgaga
    13561 aaaagaggtt aaagccaatc atcaccagca tcagccacgc tacgtgcgcc cccgcccaga
    13621 aggcgacagc aaagagaagt ggcagcagtg ccagcacgcg caaggcggaa atcaggcggg
    13681 aaggatcgct gacgcggggc gcgatgagca aactcccgag aaaacgggca accagacctg
    13741 cccccaataa caggccgatg gtttctggcg ttaaaccaat ccctttaagc cagacgctcc
    13801 agaaaggtaa aaaaatgccg taactaaaaa agtatgtgaa atagccgagc gccaaccagc
    13861 gcgtggattg caaaaccatg agtccctccc gtcaggaggc gttagtctgg ggttaatcga
    13921 atgttggtgc aagttgaaaa ataagaatat tcgagcctgc ttcccgatgt ctgtaacgcc
    13981 agttcacaag cactaaaaaa ctgcgatccg cgacgcatgt tgcaaaaata atgcgtaatt
    14041 atgccgttac gcttgccaaa cgttcctgca caatggcgat gaaatcgcgt aatgccagct
    14101 tcatctcccc tttcttccac gccatcagta aagcaatgga aggcacattt ccggcaatgg
    14161 gacgaaaaac aacctgcccg gtattaaaat tattcatata accgggtatc aaagtgacgc
    14221 ccagccccat gcccaccaga ttcatggtca ccagaatatt cgttgccacc tggacgatat
    14281 ttggctggct gttttcttgc gcaaaccacg ctttaacgat cggcgcaagc gaaccggaat
    14341 acgccggatc ggtactgacg aaattcacgc catccagttg cgcggcggtg atctcttttt
    14401 catgtgctaa agggtgatca accggtaaca ccaccactaa tggttcgtca aaaagctcca
    14461 gataatcaat ctccgggcta taaacaggat ggcgcattaa gccgacgtcg agttcaccac
    14521 ggcgaatttt ttcctcctgt tgcgtggtga ttaaactcac cagctcaatc aaggtgtctg
    14581 gctgtctgag acgaaacatc ggtaatactt ttggcaataa attcacttcc gccgatggca
    14641 caaagccaat ggttaattgt ctgtcttcct gaacaatttt ccgcgcccgt aatttggcat
    14701 tttccgcttg ttcgaggatt gccagcgcgt cctggagaaa acattctccc gccgccgtca
    14761 acgcgacttt gcgcttatcc ctcaccagta acggaacacc gacacagttt tcaagatcgc
    14821 ggatctggct gcttaacgaa ggctgtgagg tatgcagttt ttccgccgca cgggtaaagt
    14881 tcagtgcctg cgccactgcg acgaaatagc gtaaatgccg tagttccatc accttcccct
    14941 tgttatcgaa aaaacgtctc agctggtaga aattaactat ttcacatatt agcaaccaac
    15001 cagcaacatc cttatggcac aaaaatagaa ggtcaataca tcttatcttt caggattaaa
    15061 aaatatgacc acaccctcag atttgaacat ttaccaactg attgacaccc aaaacggtcg
    15121 ggtcactccg cgtatttata ccgacccgga catttaccaa ctggagcttg agcgtatttt
    15181 cggtcgttgc tggctatttc tcgcccacga aagccagatc ccaaaacccg gtgatttctt
    15241 taacacctac atgggagaag atgcagttgt cgtggtgcgc cagaaagacg gcagcatcaa
    15301 ggcgtttctc aaccaatgcc gccaccgggc catgcgtgtg agttatgcag attgtggcaa
    15361 cagtcgcgcc tttacctgcc cgtatcacgg ctggtcttat ggcattaacg gcgagttgat
    15421 cgatgtaccg ctggaacctc gcgcctaccc acaagggttg tgtaaatccc actgggggct
    15481 aaacgaagtt ccttgtgtgg agagttataa aggactgatt tttggcaact gggataccag
    15541 cgcaccgggc ctgcatgatt acctgggtga cattgcctgg tatctggatg gcatgctgga
    15601 tcgtcgcgaa ggcggcaccg aaattgtcgg cggcgtacaa aagtgggtga tcaactgtaa
    15661 ctggaaattc ccggcagagc agttcgccag tgaccagtat catgctctgt tcagccatgc
    15721 ttctgccgtt caggtattag gggcgaaaga tgatggcagc gataagcgcc tcggtgatgg
    15781 acaaaccgcc cgcccggtgt gggaaaccgc caaagatgcg ctgcaatttg gtcaggacgg
    15841 tcacggtagc ggtttcttct ttactgaaaa accggatgct aatgtctggg tcgatggcgc
    15901 agtttcaagc tattaccgcg aaacctatgc cgaagcagaa caacgtttag gtgaagttcg
    15961 cgccctgcgc ctggcgggtc ataacaatat attccccacg ctttcatggc taaacggcac
    16021 tgccacactc cgcgtctggc atccgcgcgg ccctgatcaa gttgaagtgt gggcgttctg
    16081 tattactgac aaagccgcct ccgatgaagt taaagccgct tttgaaaaca gcgccactcg
    16141 tgcttttggt cctgctggtt ttctcgagca ggatgactcg gagaactggt gtgaaatcca
    16201 gaaattgctt aaaggccacc gcgcccgcaa cagcaaactg tgtctggaaa tggggcttgg
    16261 tcaggaaaag cgccgcgacg acggcattcc tggcattact aactatatct tttcagaaac
    16321 tgccgctcgt ggaatgtacc aacgctgggc cgatcttctg agtagcgaaa gctggcagga
    16381 agtgctcgat aaaaccgccg cttaccagca ggaggtgatg aaatgagtgc gcaagtttca
    16441 ctggagttac atcaccgcat tagccagttt ctctttcacg aagccagtct gctggatgac
    16501 tggaaatttc gtgactggtt ggcacagctc gacgaagaga ttcgttacac catgcgcacc
    16561 acggttaacg cgcaaacacg cgaccgtcgc aaaggcgtcc agccgccgac gacctggatt
    16621 ttcaatgaca ccaaagacca gctggagcgg cgaatcgccc gtctggaaac gggcatggcc
    16681 tgggcagaag agccgccgtc acgcactcgt cacttaatca gcaactgcca ggtaagcgaa
    16741 accgacatcc caaacgtatt tgccgtgagg gtgaattatc tactttatcg ggcacaaaaa
    16801 gagcgcgatg aaacattcta tgttggaacg cgtttcgaca aagttcgccg tctggaagat
    16861 gacaactggc gcttgctgga acgggatatc gtcctggatc aagcggtgat cacttcccat
    16921 aacctgagtg tactgttctg atgaatcgaa tttatgcgtg tcccgttgcg gatgtgccgg
    16981 agggtgaggc tctccggata gatacctcgc ccgtcatcgc cttgttcaac gtgggcggcg
    17041 agttttatgc cattaacgat cgttgcagcc atggtaatgc gtcgatgtca gaagggtatc
    17101 tggaagatga cgccacggtg gagtgcccgc tacacgccgc cagtttttgc ctgaaaacgg
    17161 ggaaagcgtt atgcctgccc gccaccgatc cgctcaccac ttatccagta cacgttgaag
    17221 gtggtgacat tttcatcgac ttaccggagg cgcagccatg agcgatctgc ataacgagtc
    17281 catttttatt accggcggcg gatcgggatt agggctggcg ctggtcgagc gatttatcga
    17341 agaaggcgcg caagttgcca cgctggaact gtcggcggca aaagtcgcca gtctgcgtca
    17401 gcgatttggc gaacatattc tggcggtgga aggtaacgtg acctgttatg ccgattatca
    17461 acgcgcggtc gatcagatcc tgactcgttc cggcaagctg gattgtttta tcggcaatgc
    17521 aggcatctgg gatcacaatg cctcactggt taatactccc gcagagacgc tcgaaaccgg
    17581 cttccacgag ctgtttaacg tcaacgtact cggttacctg ctgggcgcaa aagcctgcgc
    17641 tccggcgtta atcgccagtg aaggcagcat gattttcaca ctgtcaaatg ccgcctggta
    17701 tcctggcggc ggtggcccgc tgtacaccac cagtaaacat gccgcaaccg gacttattcg
    17761 ccaactggct tatgaactgg caccgaaagt gcgggtgaat ggcgtcggcc cgtgtggtat
    17821 ggccagcgac ctgcgcggcc cacaggcgct cgggcaaagt gaaacctcga taatgcagtc
    17881 tctgacgccg gagaaaattg ccgccatttt accgctgcaa tttttcccgc aaccggcgga
    17941 ttttacgggg ccgtatgtga tgttggcatc gcggcgcaat aatcgcgcat taagcggtgt
    18001 gatgatcaac gctgatgcgg gtttagcgat tcgcggcatt cgccacgtag cggctgggct
    18061 ggatctttaa ggaagcacga tgaaagaaaa aacgatcatt attgtcggtg gcgggcaagc
    18121 ggcggcaatg gctgcggcct cgctacgcca gcaagggttc accggtgagc tgcatctgtt
    18181 ttccgatgag cgacatcttc cttatgaacg acctccactc tcgaaatcca tgttgctgga
    18241 agattccccg cagttacagc aggtgttacc cgctaactgg tggcaggaaa acaatgttca
    18301 tctgcattcc ggtgtaacca tcaaaacgct gggtcgcgac acacgagagt tagtgttaac
    18361 caacggcgaa agctggcact gggatcagct ttttatagca accggcgcgg cagctcgacc
    18421 gctgccgttg cttgatgcac tgggagaacg ctgctttacc ctacaccatg ccggtgatgc
    18481 cgccagactg cgagaagttc tgcagcccga acggtcagtc gtgattgtcg gtgccggaac
    18541 tattggtctg gaactggctg ccagcgccac gcagcgcagc gcagcgcagc gcagatgtaa
    18601 ggtgacagtg attgaactgg cggcaaccgt catgggccgt aatgcaccac cgcccgtgca
    18661 acgctatctt ttacagcgcc atcagcaggc tggtgtgcgc attctgctca ataatgccat
    18721 tgaacatgtg gtcgatggtg aaaaagtaga actgacgctg caaagtgggg aaacgcttca
    18781 ggctgatgtg gtgatttacg gtattggtat cagcgccaac gagcaactgg ctcgcgaggc
    18841 caaccttgat actgccaatg gcattgtcat tgatgaggct tgccgcacct gcgatcccgc
    18901 gatctttgcc ggtggcgatg tggcaatcac tcgtcttgat aatggtgcac tacaccgctg
    18961 cgaaagctgg gaaaacgcca ataaccaggc gcaaattgcc gctgccgcaa tgttagggct
    19021 accgctaccg ctactgccgc cgccgtggtt ctggagcgat cagtacagtg ataacttaca
    19081 gtttattggc gatatgcgtg gcgatgactg gctttgtcgt ggcaacccgg aaactcagaa
    19141 ggcgatttgg tttaatctgc aaaacggcgt gcttatcggt gcggtcacgc tgaatcaggg
    19201 ccgtgagatt cgcccaattc gcaaatggat ccagagcggc aaaacgtttg atgcgaaact
    19261 gctgatagat gagaacatcg cgcttaaatc actgtaatca ggataattag cgaatatctc
    19321 aatgcctggg gcgtggcgag gtgcaagagt gtgtattacg tttaaatcac attatcttgc
    19381 aaagggattg gttatgaaca cactacgtta ttttgatttt ggagctgccc gccccgtttt
    19441 gttattaatt gcccgtatcg ccgtggtctt aattttcatt atttttggtt ttcccaaaat
    19501 gatgggcttt gacggtacgg tccaatatat ggcctcgttg ggcgcgccaa tgccgatgct
    19561 ggcagcgatt attgcggtag ttatggaagt gcccgccgcg atattaatcg tgcttggctt
    19621 tttcacccgt ccgctggcgg tgctgtttat tttctacacg ctgggtacgg cggtgattgg
    19681 tcaccattac tgggatatga ccggcgatgc ggttgggcca aatatgatta atttctggaa
    19741 gaatgtcagt atcgctggcg cgttcttgct attggcaatt accggaccgg gggcaatttc
    19801 tctcgatcgg cgttaggaaa gatgccggat gcggcgtgaa cgccttatcc ggcattttat
    19861 aaattacagc cactctaccc gcaacgacat ctctgaggtt gttgattccc ctggtgctag
    19921 cgcaatgaga tcaccccctt ccgggcgatg atgctcatcc ggcgcatggc tcatcggttc
    19981 cagacagaag aaatcaaacg catatccttt atcaaacgca gggtcggaaa caaagatgaa
    20041 ataacacggt gcaggtggcg tcgtttccat gatgatggca taaccttcct gcggctgctc
    20101 gatgcgggcc tgaccattcc atccggcaaa accattgttc acccactgac gcggcaacgg
    20161 cgcgagctgg ttaaaatcca gttcctgcgg tagctgctcg caaaactcac ccgccagcca
    20221 ctgctcccgt tccagccagt aaccgctcgc ctgcgcctga atccgcgttt gtggtgacaa
    20281 cggaaaataa ggatgccagc cggtaccaaa tggcagcgtc tctgcccctt gattggtgac
    20341 agagagcgtc accgtcagcg tatccgccgt taaatgaaac gcctgactta cccgatagtg
    20401 atagacaccg ctgcgatgtt catacaccag acacaaacta tcatcgctgt gcgagacaca
    20461 ttgccactcg cccagccagc catcgccgtg tagatagtgc gcatcccact caacattcgg
    20521 ttgcagttga tactcacgcc cctgccagac aaaacggttg ccgctcaccc ggtttgcaaa
    20581 tggcaccagc ggaaaacatg aggcatcggt tgccacaccg ctttttttac caggacgtaa
    20641 gagcggcgtc gtatcgcgcc agaagccttc gattacgccg ccctggtcag aaacgtccag
    20701 ctttagcgac ccgtgggata aggtatagat ggtcatccgc taactcctta atccgggaag
    20761 ttaatcacaa ctttcccgca tttgccgctc gccatcagca cataggcatc acctgcctgt
    20821 tctagcgaga agcgatgggt aatggcgtta cgcggccaca gcttccagtc agtcaaatcg
    20881 tgggcgcatt tttccatatg gaacagactg gtcacccagg agccgataat ccgccgttga
    20941 tggtgcatca gatcagcgct gacctcgaat tccacttttc cggtttcacc aatgtaaacc
    21001 acccgccccc agtcagcagt ggattgcagt gccagcaagc gacctgcggc attaccggaa
    21061 caatcgagcg caacatccgc gccaccgtgg gtaagttcag caattatctg cggcagacct
    21121 tcggtggttg ctaaatagcc gtgatccatc ccccctaact gttttgccat cgccagacgt
    21181 tccggcagca tatcaacgcc gatgatccgt tttgcaccgc gacctttcgc cagcatcatc
    21241 gccatcatgc cgaccggccc cagaccgacc accagcacgt tatcgctgcc ggaaacttcg
    21301 ccgcgcaaaa tcccttcata ggccgtgcca acgccgcaac tgataaacgc accatcttcg
    21361 tagctcagcg catccggcag gaggatcaga tctttttctt ccgccagcag atattcggca
    21421 tgaccgccgt cacgctgcca gccgtaagcc gcttttcctt tgccagtaca agagatagga
    21481 aagccgcggc ggcagttcgg gcaaaaacca cagccagaaa tgtgatacac cagcacgcgg
    21541 tcgccctctt taaaatggcg gcagccttgc cccatcgcca caatctgccc gcacggttca
    21601 tgaccgttga taaatccctg gtataacggt ttatcgggtg ccgccgccgt ggcacggtgt
    21661 tgatgataga tatagtggac atcgcttccg caaatcccgg aggatttcat tttgatcagt
    21721 acctggttaa tccccggcgt cggcaccgca acttcccgca gatcgacggt tgaatttcct
    21781 agtaaataag cagccagcat cgttttcata aatccctcat taacaatacg attaattttc
    21841 atccctgcag cacccgcgcc aggggcgtta aggtttagcg tttcgcttta ctgcttcgct
    21901 gggtcagcaa gatattcgcc agcaccgcca ccacgatgat gacgccgcgt accacctgct
    21961 ggaaaaagga gttaataccg agcagcacca gaccgttacc gattagcgta atcaccagca
    22021 caccaagcaa tgtaccgaac agggaaccgc gaccgccgga aagtgccgta ccgccgacca
    22081 cgaccgcggc gatgacgtca aactccagac cgtttgcggc acctgcgtta ccggaaccga
    22141 ggcgtgccgc caacaaaatg ccggtcaccg ccgctaataa tcccgaaagg gtaaagataa
    22201 gaatgcgcac tcgacgaacg ttaataccgc acaactgcgc cgccgtggca ttaccgccaa
    22261 cggcaaaaac cgagcgcccg aaggcggttt ttcggctaat gaacacaaac agcgcaaaca
    22321 acaccatcat gatcagcgcg gatactggca caccgagaaa ttgtccgccc agccagtcca
    22381 gcacctcgtt ttcgtcaatc ggcactggca gtgcgttggt cataaacagc cccattccgc
    22441 gcagagcgct ccacagcccc agtgtggcaa cgaaacttgg tacgttaaac acgccgcgca
    22501 gcaccccggc gagcgttccc atcagcgcgc ctaacagcaa caccagcaga caagcaatcg
    22561 ccaacggaac ttcaaattgc agcaaaaatg ccaggcacac cgagacaaaa gccaccatcg
    22621 gcccaacgct gacatcaatt tcaccggaga taataatcag cgtcatcgcc caggcggcaa
    22681 tcccaatggt ggcggcatcg cgcagcacgt tcatctggtt attcaatgag ataaagccag
    22741 gcgcgttcag ggagaagacc agataaagaa tggcaatcac caccagcaaa ccgatctcat
    22801 taatatggcg actgacaaat tgtttgagcg agacgctctt gccctgcggc aatggtaatg
    22861 acgaagcata catcttcagg ttcctcggta aaattcatca gtgcacagac agaatggcgg
    22921 acatcagctc atccacattg accggcgagt gaaactcctg cgagaacgtg ccgtgctgta
    22981 ataacaggat gcggtcacac accagcggca attcctccac ttcactggag ataaacacca
    23041 cgctttttcc ttcggcagcc agctcacgga caatacggta aatctgctgt ttggcttcga
    23101 tatcgacgcc gcgcgttggc tcgtcgagca acaaaatctg gctggcggca tagacccaac
    23161 gaccgatcac cactttttgc tgattgccac cagaaagcgt gccgatgggt gtttcgctac
    23221 tggcagcctt gaccgtcatc cgctgcatca cctcttcggt caggcggcgg atggtggacc
    23281 attgcagcac accgttggtg ctgatttttt gccgattggt cagcactgta ttttcgtcaa
    23341 cgcctagcca gggaatgatc cccgcttctt tgcggttttc tggcgtatag ccaatgccgc
    23401 gtttcagcat gtcgccgtaa tcggggcgca tgattttctc gccgttgata acaatttcgc
    23461 cctgttcata cgtctccagc ccaacaatcg ccttcagcaa ttcactgcgc cctgccccca
    23521 gcaggccagc aatgccaagc acttcgccac gacgtagcgt aaagctgata tcctccagct
    23581 tgggcttatg gcgtaacgca cggacttcca gtacggcctg atccacaatt tcctgagggg
    23641 caaccggcgc aatatcaacg tgatcacgcc ctagcattag cgacacaata tgatgcgtgg
    23701 aggtgttttc gagcatcaca tcgcccgcca cctgaccatc gcgcataacg gtggcatagg
    23761 aggcaatgcg gcggatttct tccatccggt ggctgacata aatcaccgcc acgcccagtg
    23821 ccgacatctt tttcaccgca ctgatcacca gttcaacttc tgcactggca agcgagctgg
    23881 tgggttcatc aagaatgacc acgcgcggct cgcccttcat cacccgtgca atttccacca
    23941 gttgcttttg cgccgggctt agcgttgaaa caagttgttc aggactaacg tcaacgccca
    24001 gcgcctgtaa gcaacgttgg gcatcctgcg ccatttgcag gtaatcaatc atgccgttgc
    24061 ggcgaggcca ttgaccaagg cagaggtttt ctgccaccgt cagcccttcc accagactta
    24121 attcctgata aaccgcacga acccccagtt cagcggcacg gcgagtcaac gtagcttcgt
    24181 caccttccag tcgcgtctcg ccaatccaga tatcaccgct atccgggcgt tcgctgccgg
    24241 taagcattcg aatgagagtc gatttgcccg cgccgttttt acctaacagc gcacgaactt
    24301 cgcctttatt gagcgtgaag ttaacgttat ccagcgcaac gacgccgggg taacgcttat
    24361 ttcctgccac cacttttgct accgggactg cctctgttgc cgtgaacata gggacctctg
    24421 cgaatcagcg attagggcag accatcaacg tgcgttgcca gccactgttt accatcttcc
    24481 gttttggtat agagatcgat tggcacctga atcacttttt caccgtcggc ttgtttattg
    24541 ataaccttca atgtttgcgc gaaaacagca ttgcccattt tcttaccgga aatatccact
    24601 accgctttca gcacctgatt gttttccagc tcctgagcaa tttcggttgt catatccgaa
    24661 ccgaaaacag caatttttcc ggcctgattt tgattacgta ccgcttttac cgcgccgagt
    24721 gtcgcaccgc ccgactcccc cataatggcg ttgagatccg gcgtggagat aatcagtttt
    24781 tcaccaacgg aaatcgcttt atctaaaaca gtcccttcct ggttagcgac aatttgcgcg
    24841 ccgggaacgc gggattttaa tacttcttca aatcctttac gtcgctgcac acaaacttca
    24901 aaggcttcgc aattgatgac ggcaattttc ggctggtcaa ttttattggc aataaaataa
    24961 tcggcggcag cgttacccag tttttaccaa attccagtgg atcgccgacc agatacgccg
    25021 agacatattt atcgacaccc ttttgattaa tacaggtgtt gtagcaaatc accggaatac
    25081 ccgcttcact ggcgcgacga acggtacggc tactgccatt ttcagacact gccgataaaa
    25141 taatggcatc gacattacgc gccacgaggg tatcaacaaa ggtactttct ttcgaaatat
    25201 ccccctgggc gttagtttca attaactgta cctgtactga agaatctttt gccgcatcct
    25261 gaacgccctg acgcactcca gcgtaatatc cctgggtatc aaggtatatt gcaccaatgg
    25321 tcatttcttt atccgcagcc ctggcaaata gtgcgctacc taatagcgta gccattaata
    25381 atagattacg ggttgttctc atttttttag gcatagtgcc tcctgtaggg tttttattaa
    25441 caacggctta ttctaattat tttgtgatga gcggcagcgc gtgccgctca aatattacag
    25501 ttaatagggt ttagtgaacg tagttaaaaa taaaaggcat cacagtggtg aataatgccg
    25561 ctttcggcca ggccggatta atcgccgtca attcagcgcg agcagactca tattgcgcaa
    25621 cctccccgag tcccgcggac gccagcatcg ccaccatcag gcatttttct ttatgctgct
    25681 gttgtaaatc gccatacagc gataacaggt caggttgtga aacagcaaag aaatccgcct
    25741 caatactggt tttcgccatc tcttgcgccc actgtttcat ttcactaaac agttgctgtg
    25801 cggtttgctg ttcacccagc agtcgcagcg ccattccttg ccagaagaga taatcaaccg
    25861 gctgatcgtt gtaataactg tggatgttaa tggtgcgatc gccggtcgcc gccagacgta
    25921 aacaacgcat cgcttcagtt tcatcgccct gcgcgttggc gcatatcgcc tgccagaacc
    25981 agatgtcgtt atcagtttgc cccggtaaac ggccttcgct taaattctcc ggataataca
    26041 gcgcggcatg aagcagttcg ctggcctgct gcggctctct ggcatcaaga tgctgccagg
    26101 cgcgtaataa ctggttgagg ataaactgac tggtgacctt cccttccccg ccttcccacg
    26161 ggtggaattt acgcgtggcg agaatgtccg ccgctttgtc tgcctgaccc gtgagatgcc
    26221 acaaattgag cagttctgcg gtcatgtcgt cgcgtttcag cgcaatttcc agattatttt
    26281 ccagacgcgc cagtcgtttc tccggtgtgg ctccacttaa cttatccagc aaatcccgtt
    26341 cgaaaagcag acgtgcatcc tgcggcgcaa gctgataagc attatcaaga taacgcgcgg
    26401 ccagctcata atcgtgtttc ttattccacg catggatcgc taacccgcgc cagccgtcgg
    26461 caaactccgg cgacatctct acgcaacgtt gccataaggc aatggctttg ttgtagctac
    26521 gtttgttgta gtagaagcag gccagtaaat ggcgagcaaa ccaacactct tcaatactct
    26581 ccagcgccgc cacttcttcc agcgtattcg ggaaacggac aaactgcggg aagacatcaa
    26641 tggcttttgc gaccagttcg ccacgttcgg ctttcggcag caagctggct tgcaggtaaa
    26701 gcggcagcgt gcgctggcag tccagtgcgt tcagcatctc tgccgccagg gtgggcattc
    26761 cccagttaat cagttgcccg gcggtcatca gagcgttaac gtcgcgtccc tgacacagac
    26821 cgcgccactg cgcgagggct gattcgctac gaccatcgaa ccagttcagc caccacagag
    26881 tggcgttcag cggataatca cgcagcagtt tctcgcgctg caaacgcgcg ttgtcctgac
    26941 gaccacttaa caccagcagc agattatgca agcaaagcac ttcctgattg gttgggcagg
    27001 cacgaagact ttgttggcaa aaatccagac ccgcgtcgaa gttatcatta cgcgccgcca
    27061 gtcgtgccag accataatag ccgcctgctt tactattgcc gctccagacc gcacgccaga
    27121 aatcctcttc ggcttgttga tattgtccct gacgttcgta agcactggcg cgaatcaaac
    27181 ttgcttgtcc acactgcggt tttttgttca gcgcatgtgc acgtttcagc gcctgactgg
    27241 catacgccac cgcttgcggg aaatctgcgc ggttatattc cagcatcgcc agcgccaggt
    27301 tacagcgata atccagcggg tccagcgcca cgccgcgcag gtagtaatcg aacggtgaac
    27361 ggctggcgtg atgatattgc tccagatgct gaccgataaa ccaggcttca tctgtactgg
    27421 taatgtcttg tgctgccagt ggcgctttgg cgacgtccgg caacggcaac tcctgcggct
    27481 gatgttcctg ataactcagt acaatattgc cgtcggcatc ggagagttca atggtcagtc
    27541 tgtcaggatt gataccgtgc aacacgccct ggatggcggt cgctggtgtc agggccacgg
    27601 catcatcgag caacgcgttg catttgccga tttcgcggat cgccagacgg tatccgttca
    27661 acggagagat ggcatacagc ccccactcaa tcccccgctc actacgctgg agttttatca
    27721 ccgcatcgcg ggaggcattt tgcaccatgc ccagagaatg ataaggcagg aaatactgct
    27781 cgaaacgctt ctcttcgtaa gcatcaagcc aggtaaaatc aggctggtta tcggcaaaaa
    27841 taccggtcat cagttcgata tacgggccgt tattgtcggt cagactctta tcccacgcct
    27901 ggccaaattc actgtgtccc caactccact gttttttacc tggcgcaata tggtggttgg
    27961 caacgtgcag caaaccgcca tcttcatcgt gacaccacgc gccgacaaaa tcgtactgcg
    28021 atttttcggc catatatgag gttggaacag gcacattttt atagcgagaa atgtccactc
    28081 cggcggagta gtccactttg tagtaagtgc cggtggcgat ggggaaagcg gagacggccc
    28141 gtttgccgtg atcaaacacc gcagtcacat ccggtgggaa aacgctctga tgcccttcac
    28201 cccctttcac tgccgggttg acccaccaca agaaatgacg cggcgtggcg ttgccgttat
    28261 agacgcggct ggcgatttcc agtgccgccc ggtcagggcg cagggtgaaa cctgtcatca
    28321 cctgtaaacc atgcatcggc tccgtttcgc cgacccacac cgtctgtgca ccgtcttcat
    28381 gggcttcgag ggtgaaatca acgggcataa aggtggtcgg gcgatggtgt tgcggccagt
    28441 taaactcaat cccgccggag atccacggtc ccagcatccc caccagcgca ggtttaatga
    28501 cttcattgtg atagacaaaa tcgcgctgtt tcactttatc ccatgcgcga tgcacccgac
    28561 cgcccagttc cggcaggatc atcactttga tgtagtcgtt ttccagccac accgcctgct
    28621 aggatttcag ggttttctgc tcgctcagcg tatcggtcac gccgtaggga taaaccgcgc
    28681 cggacgatcc ctgataaacg cgattttcca ggaacatggg atgtatatcc tgcggcccgg
    28741 tttcataggt cgggatctca acgcgctctt gccacacttt tactggagtc atggtgccct
    28801 caatattaac aagacatact gaattaaaag atttgtggca gtgtattgaa caatctggca
    28861 atgttttcgc ggaataatca cgcaattaac taaacaaggt ttagtgaaga tgagagcctg
    28921 cattaacaat caacagattc gccaccataa caaatgcgtg attctggaac tgctgtaccg
    28981 gcaaaagcgc gccaataaat caacgctggc ccggctggcg caaatttcga ttccggcagt
    29041 cagtaatatt ttgcaggaac tggaaagcga aaaacgggtg gtgaatattg acgatgaaag
    29101 ccagacgcgc gggcatagta gcggtacatg gctgattgcg ccggaaggtg actggacgct
    29161 gtgcctgaac gtgacgccca ccagtattga gtgtcaggtt gctaatgctt gtttaagtcc
    29221 gaaaggcgaa tttgagtatt tacagattga tgtaccgacg ccgcaggcgc tgctgtccga
    29281 aatcgaaaaa tgctggcatc gccaccgtaa attgtggccg gaccgcacca tcaatctggc
    29341 gctggcaatc cacgggcagg ttgatccggt gaccggcgta tcgcaaacca tgccgcaagc
    29401 gccgtggaca acgccagttg aggtgaagta tctgctggaa gagaagctgg gcattcgggt
    29461 gctggtcgat aatgactgcg tgatgctggc gctggcggag aaatggcaaa ataattcgca
    29521 ggaacgggat ttctgcgtga tcaacgttga ttacggcatt ggctcgtcgt tcgtgattaa
    29581 cgagcaaatt tatcgcggca gtttgtatgg tagcggacag attggtcaca ccatcgttaa
    29641 tccggatgga gtcgtctgcg actgtggacg ttacggcagc ctggaaaccg tcgcctcgtt
    29701 aagtgcatta aaaaaacagg cgcgggtatg gctaaaatca caaccggtta atactcagct
    29761 tgagcctgaa aaactgacaa ccgcgcagtt aatcgctgcc tggcagagtg gagaaccgtg
    29821 gatcaccagc tgggttgatc gctctgccaa tgccattggt ttgagtctgt ataacttcct
    29881 caacatcctc aatattaatc agatttggtt gtacggtcgc agttgtgcct ttggtgagaa
    29941 ctggcttaat actattattc gccagacagg atttaacccg ttcgaccgcg acgaaggacc
    30001 gagcgtgaaa gcgacgcaaa ttggcttcgg gcaattaagc cgcgcacaac aggtgctggg
    30061 aattggctat ttgtatgttg aggcgcagtt acgacagatt tgatggcgcg ataacgtaga
    30121 aaggcttccc gaaggaagcc ttgatgatca taaacgaaaa attgcctgat gcgctacgct
    30181 tatcaggcct acacggagat tgcaatatat tgaatttgca agattttgta ggcctgagaa
    30241 ggtgttcatg ccgcatccaa catgaacaac gagcacattg tcagcaaatc accgtttcgc
    30301 ttatgcgtaa accgggtaac gtgcgcagat gtcgagaact ttacctttga tgcgctcgat
    30361 aacggcttca tcattgatgc tgtccagcac gtcacacatc cagccagcca gttctttcgc
    30421 ttcggcttct ttaaagccgc gacgggtaat cgccggagta cctacacgaa taccggaggt
    30481 cacaaacggg ctcttcggat cgttcggtac gctgtttttg ttgacggtga tgttagcacg
    30541 gcccagagcg gcgtctgctt ctttaccggt caggttttta tcaaccagat caaccaggaa
    30601 caggtggtta tcagtgccgc cggaaaccac tttgtagccg cgctcgagga acacttctac
    30661 catcgcttta gcgtttttcg cgacctgctg ctggtaagtt ttgaactcag gctccatcgc
    30721 ttctttcaga gcaaccgctt taccggcgat tacgtgcatc aacggaccgc cctgaccacc
    30781 agggaaaacg gcagagttca gttttttgta cagctcttcg ctaccacctt tcgccaagat
    30841 caggccgccg cgcggacccg ccagggtttt gtgagtggtg gtagtaacaa cgtgagcatg
    30901 aggaaccggg ttcgggtaga cgccagcagc aaccaggccc gcaacgtgcg ccatatcaac
    30961 gaacagataa gcaccgatgc tgtcagcgat ttcacgcatt ttcgcccagt caaccacgcc
    31021 ggaatatgca gagaaaccac cgataatcat tttcggcttg tgttctttgg cttgtttttc
    31081 cagatcggcg taatcgatat gaccggtagc atcgataccg taaggaacga tgttgtacag
    31141 tttaccggag aagttaaccg gagaaccgtg agtcaggtga ccgccatgcg ccaggttcat
    31201 acccagaacg gtatcacctg gttccagcag cgcggtgtag accgcaaagt tagcctggga
    31261 gccggagtgc ggctggacgt tagcgtagtc agcgccgaac agttctttcg cacgatcgat
    31321 cgccagttgt tcaacgatat caacatactc gcaaccgccg tagtagcgtt tgcccggata
    31381 accttcagca tatttgttgg tcagctgaga accctgcgcc tgcattacgc gcgggctggt
    31441 gtagttttcg gaggcgatca gttcgatgtg ctcttcctga cgtacttttt cctgctccat
    31501 agcctgccac agttcggcat cataatcggc aatgttcatt tcacgcttta acatccgcat
    31561 ctcctgactc agctaacaat aaaatttttg gcctttatag gcggtcctgt tggacaacgg
    31621 cgaacagtat aaccgaatca ttgtgcgata acaggtcttg acaaaggaat ttacgcaaac
    31681 gattaccttc aggctacgca aggctttgga gaataaagag cttgcaaccg gaaacggatt
    31741 tcttttcagg tttgtgatgc aaatttttca cttcatcaca ttctttctga aaaacaccaa
    31801 agaaccattt acattgcagg gctatttttt ataagatgca tttgagatac atcaattaag
    31861 atgcaaaaaa ggaagaccat atgcttgacg ctcaaaccat cgctacagta aaagccacca
    31921 tccctttact ggtggaaacg gggccaaagt taaccgccca tttctacgac cgtatgttta
    31981 ctcataaccc agaactcaaa gaaattttta acatgagtaa ccagcgtaat ggcgatcaac
    32041 gtgaagccct gtttaacgct attgccgcct acgccagtaa tattgaaaac ctgcctgcgc
    32101 tgctgccagc ggtagaaaaa atcgcgcaga agcacaccag cttccagatc aaaccggaac
    32161 agtacaacat cgtcggtgaa cacctgttgg caacgctgga cgaaatgttc agcccggggc
    32221 aggaagtgct ggacgcgtgg ggtaaagcct atggtgtact ggctaatgta tttatcaatc
    32281 gcgaggcgga aatctataac gaaaacgcca gcaaagccgg tggttgggaa ggtactcgcg
    32341 atttccgcat tgtggctaaa acaccgcgca gcgcgcttat caccagcttc gaactggagc
    32401 cggtcgacgg tggcgcagtg gcagaatacc gtccggggca atatctcggc gtctggctga
    32461 agccggaagg tttcccgcat caggaaattc gtcagtactc tttgactcgc aaaccggatg
    32521 gcaaaggcta tcgtatcgcg gtgaaacgcg aagagggtgg gcaggtatcc aactggttgc
    32581 acaatcacgc caatgttggc gatgtcgtga aactggtcgc tccggcaggt gatttcttta
    32641 tggctgtcgc agatgacaca ccagtgacgt taatctctac cggtgttggt caaacgccaa
    32701 tgctggcaat gctcgacacg ctggcaaaag caggccacac agcacaagtg aactggttcc
    32761 atgcggcaga aaatggcgaa gttcacgcct ttgctgatga agttaaggaa ctggggcagt
    32821 cactgccgcg ctttaccgcg cacacctggt atcgtcagcc gagcgaagcc gatcgcgcta
    32881 aaggtcagtt tgatagcgaa ggtctgatgg atttgagcaa actggaaggt gcgttcagcg
    32941 atccgacaat gcagttctat ctctgcggcc cggttggctt catgcagttt accgcgaaac
    33001 agttagtgga tctgggcgtg aagcaggaaa acattcatta cgaatgcttt ggcccgcata
    33061 aggtgctgta atttgatgtt gccggatgga aacatccggc aacccttgac gcggtttaaa
    33121 ttgccgcgtc gtcctcttca ccggtacgga tgcgaatgac ccgagccacg tcaaagacga
    33181 agattttacc gtcaccgatt ttgccggttt gcgccgtgcg aataatggta tcgacacagg
    33241 tatcgacaat gtcgtccggt acgacaatct caattttcac tttcggcaga aaatccacca
    33301 tatactccgc gccgcggtac agctcggtat ggcctttctg gcgaccaaag cctttcactt
    33361 cggtcaccgt catgccggta ataccgactt cggccagtgc ttcgcggaca tcgtccagct
    33421 tgaagggttt tataatcgca tcaatctttt tcatgctatt ccttgaaaag gttgcctgtc
    33481 ttttgatctg ctaaacgtaa cacataacgc caattcattc cttgaaatcg tttgcatcca
    33541 gctcgtgtcg ggaaagcagt ttataaaatt ctgtccggtt gcgccccgcc attctcgccg
    33601 cgtgggtgac gttgcctttg gtgatttgca gcagcttacg caagtagttg agttcaaaat
    33661 gattacgtgc ctcaacaaag gttggcagcg ccgtattttc accctccagc gcctgctcca
    33721 ccagcgcatc actaatcacc ggagatgagg tcagcgccac gcactgttca atcacgttga
    33781 ccaactggcg cacattaccc ggccagctcg cggtcatcag gcgtttcatc gcatcggtag
    33841 agaacgcgcg gacaaacggt ttatgtcgct ctgccgcctg gcgcaacagg tgatttgcca
    33901 gtagcggaat gtcttctgtg cgctccgcca gtgccggaat tttcaggctg acaacgttga
    33961 ggcggtaata gagatcttcg cggaattccc cgcgcgccat cgcttttgac agatcacggt
    34021 gagtggcaga aataatccgc acattgatat caatatcgcg gttactgccc agcgggcgca
    34081 ctttacgctc ctgcaacacg cgcagcagtt tgacctgtaa cggcgcaggc atatcgccaa
    34141 tttcatcgag aaatagcgtt ccgccttccg ccgcctggaa taaaccttcg cgattgctga
    34201 cagcgccagt aaacgcgcca cgcgcatgac caaacagctc cgactccagc aattgctcgg
    34261 gcaatgcgcc acagttaata gcaataaatg gtttgctgtt gcgcgggctg gcgttgtgga
    34321 tagcctgggc gaaaatctct ttcccggtgc cgctctgacc gttaatcaaa acgctgacgt
    34381 ctgattgcgc caccagccgc gcctgttcca gcaaacgcag catcagcggg ctgcgggtga
    34441 caattgcctc gcgccagcgt tcatcggtgg ctggcgcgga ttgctccagc gcatcgtcaa
    34501 ttgcctgata tagcgcatct ttatcgacag gcttggtgag gaaactaaaa acgccctgct
    34561 gtgttgcagc aacggcatcg gggatagaac catgcgcagt aagaataatt actggcattc
    34621 ccggctgcac tttctggatt tcagcaaata gctgcatacc gtccatttca tccatccgca
    34681 ggtcgctgat aactaaatct actttttcgc gattcagtac ccgtaatcct tcagcgccac
    34741 tttccgccgt gaccacactg tagccttcgc tggtcaggcg caggccaagc agtttcagca
    34801 atcccggatc gtcatcgacc aataataaat gcgcaggttt atggctcatc aggagtgacc
    34861 tcatgggtgg atggcgcggg cttttcactc tcgtgcggcg tatccggcga gaaatttcca
    34921 gccggtttgc gggtcgagag ctggcgttca atatcggtca ggttttccag cttgcgggtg
    34981 gtgagttcca gttgctgctg tagaacgtga tgttgctggc gcaatgtatc cagctcgctg
    35041 tcgctggact gctggagttt actgtaacgt tggcgctctt ccgccagttg cagttgcagc
    35101 gcctgaccgt cgcgccagag ttgatacagt gggcgaacct gtgccgggat ctcggtactt
    35161 aacgcttcaa tacgcgcgac cagctggcgg cgctcatacg gcgtaatttt ggcgtcggcg
    35221 agcaaaatcc cttgtttaaa ggtattttgc cagctgccgt cgtcatattg gcgagcttgc
    35281 tgacgcgact gcgcaggcat taaacgatca gcacaatcca tcgcccgcag ccagtaaagc
    35341 ggattggttt cggttgattt accttgcagc gcccagatgt cgctacattc agtagaaaga
    35401 tagtcagcca gttgataaac cggaattttt tcttctgctg gcgtatcaat ggctggctta
    35461 ttgtgattct gcacgcaacc cagcaatgcc agacatggca gccctgccag ccacagccgt
    35521 cggggcaata atcgttgaaa aatgtgtcgc atattcacca gacttaaagc ctatcccagt
    35581 gggcgtaatt gttgcagaca gtctggacat ggacagcgcg gagaaaccgg ggcgtacata
    35641 tcgtacgtga agatttcgag cactacccgg ggccgaactg acaaataaaa tagcctgata
    35701 ggataggctg ttagcattat ttcgtgtttt tcgacgacgg taattcaatg cggaaacaaa
    35761 cgtcttgccc gctctcgtcg accagataca gttccccttg catacggcga atacaatccc
    35821 tggcaatgct taatcccaga ccgctgccct tcaccgcccc ttttcgctgg tggcttccct
    35881 gaaaaaaggg ttcgaagatc atggcgcgtt cctcttgcgg aatgggcgtg cctgtattga
    35941 tgacatcaat ataaacccgc gcaccatgta aactgctgcg aaggcaaatg ttaccggatt
    36001 cagccccgta gtgcaccgca ttggagtaaa gattatccag tacgctcatc agcagcattg
    36061 gctccgccag gcaagctgtt gctttgagat cgacgtcggt atgcatcatt ttagcccgtg
    36121 cgggcaggct atgagcagaa accactgtct ccaccagcgg tgctaattca acattctcca
    36181 gttccaccgc actgtccgcc tgtttacggt tgtaatcaag cagttgttcg atcagttttt
    36241 gcaaattgca gctgctgcta tcaagaatgc tcaccacctc tttttgctct ggcgtaagcg
    36301 gcccgacaac ctgatcagcc agtaattcag tgccctcgcg catactcgcc agtggcgttt
    36361 ttaattcatg agataaatgt cttaaaaatt gatggcgttg ggattccagc catgacaggc
    36421 gctcacttaa ccaaagaata cgttgcccaa ccgagcgtaa ctcgctcggt ccacagaacg
    36481 aaacgctatt gcccagagaa cgcccttccc ccagacggtt gatcatgcgc tcgatatttt
    36541 tcaccggccc gataatcatc cgcgtgaaaa gtagcaccat caccagactc accagaaata
    36601 gcaccagcga ttgccaacca aaatattgcc cacgttcggc gatttcacgc tgaagttgct
    36661 gcccacgaga gaacaccact gtgcgcgtgg cctgcaccat ttcggtattg gcactggcaa
    36721 aggcttcaag acgcgcggcg gcggcagcat cgggaccact gttgttacac tgaagttggg
    36781 ccagattgtt caaatcctga cgcaatgcct ggtaaagttt atcgtctggc agtacgcctg
    36841 cgtgggcatc gagcatttcg ctgtaacgct tgcgctggct ttgataaacc ttcgcaagcg
    36901 tagggtcgtc cagcacgcaa tactgacggt agctacgctc catctccagc gccgcgttgg
    36961 tcatcgcttc actacgccgg gcatcaataa gcgtagtgcg gttaaccagc gccgcctgat
    37021 cgctaagcgc attcaggctt tgccaggctt gccatgccag caccaacagg ggtagtagaa
    37081 ttagcaaaaa tgccagcatt accagttgtc gtaatgagcg gggaaaaacg ggccagcgtt
    37141 tcaaggtgtt actctcgtca gacgcgaata gcctgatgct aaccgagggg aagttcagat
    37201 acaacaaagc cgggaattac ccggctttgt tatggaataa ggcggtgcct aactcgacgt
    37261 ttcgcccgat ggttgatata gctacgctga tatcagaagc tggacggcag gcaccttgtt
    37321 gtgcgtcatt cgaattttat gtagcacgtc ccgaaggggc tgacataagt cggtgaatga
    37381 gccactgctt actattatgc agtaactgtg ccaataaaga aaatagtttg gtaacgtact
    37441 gattatacgt tgctttgagg gatttatggc ttcttcgcta tttgaatgta aatcagccac
    37501 tgtgtcgcta aaaagagaca acttaagata aacttattag attgcaataa aatcaatcat
    37561 ttaagtgtcg ccagaaagcg acacggtaaa ccacccattg tcgtgattta cagacacaaa
    37621 aaagctcccg gagttgggag cttatgatag tggttggtgc ttaatgccgc atccgggctg
    37681 caaaaccaat gaactgatga cttaccccaa ctgcttacgc gcattgcgga aaatgcgcat
    37741 ccatgggcca tcctcgcccc agttttccgg atgccaggag ttgctgacag tacggaaaac
    37801 acgttccggg tgcggcatca taatggtgac tcgaccactt tcagtcgtaa ccgccgtaat
    37861 accgttcggt gaaccgttcg ggttagccgg gtaggtttca gtgactttgc cgaagttatc
    37921 gacatagcgc agtgccacca gccctttgct ttccagtgcc gccagatgcg ccgcatcacg
    37981 cacttccacg cgcccttcac catgagagac agcaatcggc atttgcgagc ccaccatccc
    38041 ctgcaacagc agagacgggc tttgggttac ttcaaccagg ctgaaacgcg cttcaaagcg
    38101 atcggaggtg ttacgcacaa aacgtggcca caactcactc cccgggatca gttcacgcag
    38161 gttagacatc atctggcaac cgttacatac ccccagggcc agcgtttgcg gacgatggaa
    38221 gaaggttgca aactcatcgc gtacacggtc attgaacagg atagacttcg cccaaccttc
    38281 accggcaccc agcacatcac cgtaggagaa acctccgcac gcgaccaggg cgtgaaaatc
    38341 ctccagtccc gtacgtccag tcagcaggtc gctcatatgc acgtcgatag catcaaagcc
    38401 cccacgttgg aaagctgccg ccatttcaac atgcgagttc acgccctgct cacgcagtac
    38461 ggcaactttc ggacgtgcgc cagtggcaat atacggtgct gccacatctt cgttgatatc
    38521 gaacgacagt tttacattca ggcccggatc ggcgtcgtta gatttcgcct gatgctcctg
    38581 atcggcacac tccgggttgt cacgcaggcg ctgcatctgc caggtagttt ctgcccacca
    38641 gacacgcaac gtagtgcggc tttcgctgaa tacagtctgc ccgttggcgg taatcacaaa
    38701 acggtcaccg gaaaccgcct gcccaacata atggacgcaa tccgcaagcc catgctgtgc
    38761 cagtacggac tcgaccgctt cacggtcagc ggcacgaacc tgaatcaccg cacccagttc
    38821 ttcgttaaac aacgccgcca ggcgatcgtc acccagagag gcgatatccg cgtcaatgcc
    38881 acaatgacct gcaaaggcca tttccgccag cgttaccagc aggcctccat cagagcggtc
    38941 gtgatacgcc agcagcttac gctgtgcaac cagcgcctga atcgcgtcat agaagccttt
    39001 cagttggaca acatcgcgta catctgccgg tttgtcgcca agctgacgat aaacctgcgc
    39061 cagcgccgtt gcgcccagcg cgttattgcc tttgcccaaa tcaatcagca gcagtgcgtt
    39121 atcttcggta caaagctgcg gcgtgatggt gtgacgtaca tcttccacgc gggcaaatgc
    39181 agaaatcacc agcgacagcg gcgacgtcat ttcgcgctct tcgttacctt cctgccagcg
    39241 ggttttcatc gacatagagt ctttacccac cgggatcgtc aggcccagcg ccggacaaag
    39301 ttcttcaccc acggctttaa cggcttcata caggcccgca tcttcaccag ggtggcctgc
    39361 cgccgccatc cagttggcgg aaagtttaat gcgtttgata tcgccaatct gtgttgcggc
    39421 aatattggtt aacgcttcac cgaccgccag acgggcagag gcggcgaaat ccagcagcgc
    39481 aaccggcgca cgctcgccaa tcgccatcgc ttcaccgtag tagctgtcga ggctggcggt
    39541 agtgaccgcg cagttagcga ccggcacctg ccacggcccc accatctgat cgcgcgccac
    39601 catgccggtt acgctgcggt caccgatggt caccaggaaa gttttctccg ccacggtcgg
    39661 cagatgcagt acgcgtttca ccgcgtctgc aatggtgatc ccttcacgga ccagcgcatc
    39721 gcccttcgct ttcagcgttt gtacatcgcg ggtcatcttc ggcgttttac caagcaggac
    39781 gtccagcggc agatcgatcg gctgattatc aaaatgacga tcgtgcagag aaagatgcag
    39841 ttcttcggtc gcttcaccaa tcaccgcgta gggtgcgcgc tcacgcttac acagttcgtc
    39901 aaacagcggt aattgatcgg cagcaaccgc cagcacgtag cgttcctggg attcgttaca
    39961 ccagatttcc agcgggctca tgcccggttc gtcgcttaga atatcgcgca gttcaaattt
    40021 accgccgcgt ccgccgtcgc tcaccagctc cggcatcgcg ttagaaagac cgcccgcacc
    40081 gacgtcgtgg ataaacagaa ttgggttagc atcaccaagc tgccagcaac ggtcgatcac
    40141 ttcctggcaa cgacgctcca tctccgggtt gtcgcgctgt acggaagcaa agtcgaggtc
    40201 ggcatcagac tgaccagacg ccatggaaga cgccgcgccg ccgccaagac cgatgttcat
    40261 tgccgggccg ccgagaacga ccagcttcgc accgacgttg atctcgcctt tttgtacgtg
    40321 atcggcgcga atgttgccga tcccgcccgc cagcatgatc ggtttgtgat aaccgcgcag
    40381 ctcttcgcca ttgtggctgt tcactttttc ttcataagta cggaagtagc cgttcagtgc
    40441 cggacgacca aattcgttgt taaacgccgc accgcccagc gggccttcgg tcatgatgtc
    40501 cagcgcggta acaatgcgct caggcttacc gaaatcttct tcccacggct gttcgaagcc
    40561 aggaattcgc aggttggata cggagaaacc aaccaaacca gctttcggct tcgcgccgcg
    40621 cccggtggca ccttcatcgc ggatttcacc gccggaaccg gtcgccgcgc ccggccacgg
    40681 agaaatcgcc gtcgggtggt tgtgagtttc gactttcatc aggatgtgcg ccggttcctg
    40741 atggaaattg tagcggcccg tttcgtggtc agcaaagtag cggcccactt cagaaccttc
    40801 cattacggcg gcgttatctt tataagcaga gagaacgtga tctggcgtgg tttcgaaagt
    40861 atttttgatc atcctgaaca gcgatttcgg ctgttgttca ccgtcgataa tccagtcggc
    40921 gttaaaaatt ttgtggcggc agtgctcaga gttcgcctga gcgaacatat acagctcgat
    40981 gtcgttcggg ttacgaccaa gctttgtgaa agcatcctgc agatagtcaa tttcatcttc
    41041 cgccagagcc aagccaagac gcaggttagc gtcgatcagc gcctgacggc cctgccccag
    41101 caaatcaacg cttgtgaccg gagtcggttg atggtgggca aacaactgct ctgcatcatc
    41161 taaatcaaaa aagaccgttt ccatcatgcg gtcgtgcagt tcagcggtaa cctgctgcca
    41221 ttgttcattg gtcagcgtac cggcttctat atagtaagca acgccgcgct caaggcggtt
    41281 tacctgttgt agcccgcagt tatgggcaat atcggtcgct ttcgaagacc agggagagat
    41341 ggtgccagga cgcggggtca ccagcaggag tttgccttgc ggggcgtggc tggcgagtgc
    41401 cggaccatat ttcagcaggc gttcaagttg tgcgtgctca tcatcgttta acggcgcatt
    41461 gaggtcagca aaatggacat actcggcgta aatattgtga accgggagcc tggcagcctg
    41521 aaaacgtgcc agcagtttgt tgattcggaa tgccgacagt gcaggcgaac cacgcagaat
    41581 ttccatcata agtctctcgt cttctaagct ttcggtgtac ccaagggggg ggaaacgggc
    41641 gtcattataa agaatctgat gcgctgacga aaccgtttgc gtggaaataa aatcaccatc
    41701 gtgaattagc aacgcgtgcc gccaatggct gtaataagtt gccatctggc gcaggtttac
    41761 gcaaaatgcc gctcatttat gagtaaacct ttcactatta ttacgttttt tcaagctggg
    41821 acgcgcacga cacagagaat taactaattg aaaaaattaa agattaatta tctgttcatc
    41881 ggcattctgg cactgctgct cgcggtcgct ctctggccat ccattccctg gtttggtaaa
    41941 gccgacaacc gtatcgccgc cattcaagcg cggggagagt tgcgtgtgag caccattcat
    42001 actcccctga cttataacga aatcaacggg aaaccttttg gcctggatta cgaactggcg
    42061 aaacagtttg ccgattacct cggcgtaaaa ctgaaagtga ccgtgcggca gaatatcagc
    42121 cagctgtttg acgaccttga taatggtaac gccgacctgc tggcggcagg actggtctat
    42181 aacagtgagc gggtaaaaaa ttatcagcct ggccctacct attattccgt gtcacaacaa
    42241 ctggtttata aagtgggtca gtatcgccca cgtacgctgg gcaacctgac ggcggagcaa
    42301 ctcaccgttg caccgggtca tgtggtagtt aacgatctcc agaccctgaa agaaacaaaa
    42361 ttcccggaat taagctggaa ggtagacgac aaaaaaggct ctgcggaatt aatggaagat
    42421 gtcatcgaag gaaaactcga ttacaccatt gctgattctg tcgccatcag cctgtttcag
    42481 cgcgttcacc cggagctcgc cgtagcgctc gatatcaccg atgaacaacc ggtgacttgg
    42541 tttagcccgt tagatggcga taataccctt tccgccgccc tgctcgactt cttcaacgaa
    42601 atgaatgaag acggtacgct ggcacgcatt gaagagaaat acctggggca tggcgatgat
    42661 tttgattacg tcgatacgcg cacattttta cgcgccgtcg atgcggtact gccgcagtta
    42721 aagcccctgt ttgagaaata cgccgaagaa attgactggc gtttgctggc cgctattgct
    42781 tatcaggaat cgcactggga tgcacaggcc acttcaccga cgggtgtgcg cggcatgatg
    42841 atgttaacca aaaataccgc gcaaagcctc ggcattacgg atcgtaccga tgccgaacag
    42901 agcatcagcg gtggcgtgcg ttatttgcag gatatgatga gtaaagtgcc ggaaagtgtg
    42961 ccggagaacg aacggatctg gtttgccctc gctgcgtaca atatgggcta tgcgcatatg
    43021 ctggatgccc gcgccctgac ggcaaaaacc aaagggaatc ctgacagttg ggctgacgta
    43081 aaacagcgtc tgcctttact tagccagaaa ccctattaca gcaagctgac ttacggctac
    43141 gctcgtgggc atgaagccta cgcttatgtc gaaaatattc gtaagtatca gattagcctg
    43201 gtgggttatc tgcaagagaa agagaagcag gctacagaag cggcgatgca actggcgcag
    43261 gattatccgg cggtatcgcc tacggagttg ggcaaagaga aatttccttt tctctcgttt
    43321 ctttcccagt cgtcatcaaa ctatttgacc cattctccct ctctgctgtt ttccaggaaa
    43381 gggagtgaag agaaacaaaa ttaatccgtc gaggattgcg cttttttctg cgctttaatt
    43441 tcctggcggc gcatgcgaaa gaagtcactg agcaacgccg cgcactcatc cgccagtatt
    43501 ccttccgtaa tttccactcg gtgattcata cccggatgat gcagcacatc cattaaagat
    43561 cccgcagcgc cagttttcgc gtcacgcgca ccaaagacca cgcgaccaat gcgactgtgg
    43621 atcatcgctc cggcacacat tacacatggt tcaagcgtga catacaacgt ggcgtcgatc
    43681 agacgataat tttgcatcac cagaccaccc tgccgcaggg ccatgatttc tgcatgtgcg
    43741 gtgggatcat ggcgaccaat cgggcggttc cagccttcgc cgattacccg attgttatgc
    43801 actaataccg cgccgaccgg cacttcccgc tcatcccagg cacgtttcgc cagcgtcagc
    43861 gcgtgacgca tccaatattc gtggctaaat tcgacttcag acaaaaagaa aactccggtt
    43921 ataaaagcgc ggcgcattat acacggacgc tatgctttac tctattccag ttgctggagt
    43981 tcaccgcgcg gggttactcg ccaacgatgc tgacagaaat aaagcagcgg attgtcctga
    44041 ttactgtcgc tatagccact gtacagccgc agcggcgtgc cgattttgcg ctccagttgt
    44101 gcgacctttt catgtcccag acaacgcatc gtcaataccc aaccaccata gccacgctga
    44161 atttggctgg cgataagatt aacccgcggc agccagggcg tatcgaaata aaccgcttca
    44221 accagcggct gcggagagcc ggtaatcaac cagatatcag catcggaact taacaggtag
    44281 gtggttaatc gctcctgaac cagcggaaag gcggtaacat tgtcgcgaaa ccagcgcacg
    44341 aaatcggcct gcaacgtctg taaacgtgct tcgctgtgac caaaagtgca cccccacaga
    44401 agcagactca tcggccagcg tgccgcacga ccttttatca ataacgcaat ggctataatc
    44461 ggtaacaacg gcaggacaag taacgcattc agcggttggc gacgcagtaa atagcgcaga
    44521 aaactgccga acatatcctg ctgatgcaat gttccatcta agtcaaaaaa caccacacga
    44581 cgctcgtgag ttgccaaacc atactcctct ggactgaaac atcctgatta attttcgcta
    44641 ctacatagcc taacagatag atcatcactt ttccggcaac aatcgaccgc atcatggcta
    44701 actggaattt ttaattcatg gcaattagcg gcaatggaat ataaaattca ctcgcgtgtg
    44761 tctcatattg cctgttgtcg ccactatttc atcgcgagga aagatatgaa cggcttactt
    44821 cgtatccgtc agcgttacca agggcttgcc cagagtgata aaaaactggc ggattatctg
    44881 ctgctacaac ctgatacggc gcgccattta agctctcagc aactggccaa cgaagccgga
    44941 gtcagtcagt ccagcgtcgt gaagttcgcg caaaaactcg gctataaagg ttttccggcg
    45001 cttaagttgg cgttgagtga agcgctggca agccagccgg aatcgccctc cgtgcccatt
    45061 cataaccaaa tccgcggtga tgatccgtta cggctggtcg gcgaaaaact gattaaagaa
    45121 aataccgccg ctatgtacgc aacgctaaac gttaatagtg aagagaaact gcatgaatgc
    45181 gtaacaatgt tgcgctctgc gcggcggata attctgaccg gtattggcgc ttcgggtctg
    45241 gtggcgcaaa actttgcctg gaagctgatg aagattggct tcaatgctgc cgcactgcgc
    45301 gatatgcatg cgctactcgc aacagtacag gcgtcgtccc ctgacgatct gttattagcc
    45361 atttcctaca ccggtgtacg acgcgagtta aacctggcgg cagatgagat gctgcgagtg
    45421 ggcggaaaag tgctggcgat taccggcttt actccgaatg ccctgcaaca gcgtgcttct
    45481 cattgcctgt ataccattgc cgaagaacaa gcgacaaaca gtgcttcaat ctctgcttgt
    45541 cacgctcagg gaatgttaac ggatttgctg ttcattgcgc tgattcagca ggatctggaa
    45601 ctggcaccag aacgtattcg tcatagtgaa gcgctggtga aaaaactggt ctgagtaaag
    45661 aatgcgcgta taatgcccgc ccggtttgtg ttgttttgag agtttcctta tggcgctgtt
    45721 aatcactaaa aaatgcatca attgtgatat gtgtgaaatc aaataaaaac acatttattt
    45781 tcatagtgat acagcaatgt gttattgaat gtgttgttgg atttatggtg caaaaaaatt
    45841 ttgcataagt tcagcaacac aaagaacacc actttcttta acgtcctccg cgccctaaag
    45901 gacggggctt tacggcgcaa aaggcctgtg acatacaaca ttaacttttc agtcaaccgt
    45961 agcacgttct tgtatacgcc gtgtctgcgg ttttatataa ccctggctgg aaacctctta
    46021 tacaaagtcg attcgccaac atcatagatg atcgccacct tctggcgaga aactcctgat
    46081 gcaattagtc gcccggcctg cgcccattat tctggtgaaa gtttgggacg gcgaccgcca
    46141 attcgtccct gtgcgcgagc agcttccagt cctgcttttg ttcgttcaac aatcagttca
    46201 cgttccattt cagccagggc acccatcaca tggaagaaaa agcgtcccat tggtgtgctg
    46261 gtatcaattg aatcagtcag actacgaaag ttgatgcctc gttcgcgcaa cgcctccacc
    46321 agcacgacaa gatgccgcat actgcgcccc agccgatcca gcttccagac aaccagtgtg
    46381 tcgcctgccg ataatgtctt gagcagtttt ttcagccccg gtctttcgga ctttgtacca
    46441 cttatcttgt cttcaaaaat cagctcgcat cctgcacagt tcaacgcatt ac
//
LOCUS       DAZPCS010000018        44435 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZPCS010000018 DAZPCS010000000
VERSION     DAZPCS010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 44435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44435
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.018"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            50..1816
                     /gene="adeD"
                     /locus_tag="ACHUQ7_000950"
     CDS             50..1816
                     /gene="adeD"
                     /locus_tag="ACHUQ7_000950"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFR1134471.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     AGEISGPIVIKGRYIAGVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVINGQNALLDKLDAFRYLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAASNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLALSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(1862..3253)
                     /gene="uhpT"
                     /locus_tag="ACHUQ7_000951"
     CDS             complement(1862..3253)
                     /gene="uhpT"
                     /locus_tag="ACHUQ7_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFR1134472.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3391..4710)
                     /gene="uhpC"
                     /locus_tag="ACHUQ7_000952"
     CDS             complement(3391..4710)
                     /gene="uhpC"
                     /locus_tag="ACHUQ7_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFR1134473.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(4720..6222)
                     /gene="uhpB"
                     /locus_tag="ACHUQ7_000953"
     CDS             complement(4720..6222)
                     /gene="uhpB"
                     /locus_tag="ACHUQ7_000953"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFR1134474.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHLVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(6222..6812)
                     /gene="uhpA"
                     /locus_tag="ACHUQ7_000954"
     CDS             complement(6222..6812)
                     /gene="uhpA"
                     /locus_tag="ACHUQ7_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFR1134475.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(6887..7177)
                     /gene="ilvN"
                     /locus_tag="ACHUQ7_000955"
     CDS             complement(6887..7177)
                     /gene="ilvN"
                     /locus_tag="ACHUQ7_000955"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFR1134476.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIAKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(7181..8869)
                     /gene="ilvB"
                     /locus_tag="ACHUQ7_000956"
     CDS             complement(7181..8869)
                     /gene="ilvB"
                     /locus_tag="ACHUQ7_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFR1134477.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAVAPAFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPRVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(8975..9073)
                     /gene="ivbL"
                     /locus_tag="ACHUQ7_000957"
     CDS             complement(8975..9073)
                     /gene="ivbL"
                     /locus_tag="ACHUQ7_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFR1134478.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            9638..9727
                     /gene="tisB"
                     /locus_tag="ACHUQ7_000958"
     CDS             9638..9727
                     /gene="tisB"
                     /locus_tag="ACHUQ7_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFR1134479.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            complement(9851..9925)
                     /gene="ysdE"
                     /locus_tag="ACHUQ7_000959"
     CDS             complement(9851..9925)
                     /gene="ysdE"
                     /locus_tag="ACHUQ7_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFR1134480.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            10007..11191
                     /gene="emrD"
                     /locus_tag="ACHUQ7_000960"
     CDS             10007..11191
                     /gene="emrD"
                     /locus_tag="ACHUQ7_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFR1134481.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(11199..11696)
                     /locus_tag="ACHUQ7_000961"
     CDS             complement(11199..11696)
                     /locus_tag="ACHUQ7_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFR1134482.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(11693..12055)
                     /locus_tag="ACHUQ7_000962"
     CDS             complement(11693..12055)
                     /locus_tag="ACHUQ7_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFR1134483.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(12045..12392)
                     /locus_tag="ACHUQ7_000963"
     CDS             complement(12045..12392)
                     /locus_tag="ACHUQ7_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFR1134484.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            12501..12914
                     /gene="yidI"
                     /locus_tag="ACHUQ7_000964"
     CDS             12501..12914
                     /gene="yidI"
                     /locus_tag="ACHUQ7_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HFR1134485.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(12961..14403)
                     /locus_tag="ACHUQ7_000965"
                     /pseudo
     CDS             complement(12961..14403)
                     /locus_tag="ACHUQ7_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(14438..16153)
                     /locus_tag="ACHUQ7_000966"
     CDS             complement(14438..16153)
                     /locus_tag="ACHUQ7_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFR1134486.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAIGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLEGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYVGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWWHRHDPAVTFTPD
                     AKDSL"
     gene            16320..17213
                     /locus_tag="ACHUQ7_000967"
     CDS             16320..17213
                     /locus_tag="ACHUQ7_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFR1134487.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHSNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDISVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMSADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(17210..18024)
                     /locus_tag="ACHUQ7_000968"
                     /pseudo
     CDS             complement(17210..18024)
                     /locus_tag="ACHUQ7_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(18024..19640)
                     /locus_tag="ACHUQ7_000969"
     CDS             complement(18024..19640)
                     /locus_tag="ACHUQ7_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFR1134488.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            19935..20609
                     /locus_tag="ACHUQ7_000970"
     CDS             19935..20609
                     /locus_tag="ACHUQ7_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFR1134489.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLMPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(20648..22309)
                     /locus_tag="ACHUQ7_000971"
     CDS             complement(20648..22309)
                     /locus_tag="ACHUQ7_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFR1134490.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(22505..22933)
                     /gene="ibpB"
                     /locus_tag="ACHUQ7_000972"
     CDS             complement(22505..22933)
                     /gene="ibpB"
                     /locus_tag="ACHUQ7_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFR1134491.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(23045..23458)
                     /gene="ibpA"
                     /locus_tag="ACHUQ7_000973"
     CDS             complement(23045..23458)
                     /gene="ibpA"
                     /locus_tag="ACHUQ7_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFR1134492.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(23452..23524)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            23763..24095
                     /locus_tag="ACHUQ7_000974"
     CDS             23763..24095
                     /locus_tag="ACHUQ7_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFR1134493.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(24097..25329)
                     /locus_tag="ACHUQ7_000975"
     CDS             complement(24097..25329)
                     /locus_tag="ACHUQ7_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFR1134494.1"
                     /translation="MKQITFAPRNHLLTNTNTNTNTNTWTPDSQWLVFDVRPSGASFT
                     GETIERVNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHR
                     RGVIAEGGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERGSFTYNDHVMHQLDPA
                     LDLRNVGVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVG
                     NHALAFIGDTLSPKGEKVPELFIVELPQDEASWKSAGDAPLSGTETTLPAPPRGVVQR
                     RLTFTHHRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRKLTH
                     NKTDIQSAFNWHPSGEWLGFVLDNRIVCAHAQSGEVEYLTENHANPPSADAVVFSPDG
                     QWLAWMEGGQLWITETDR"
     gene            25430..26530
                     /gene="cbrA"
                     /locus_tag="ACHUQ7_000976"
     CDS             25430..26530
                     /gene="cbrA"
                     /locus_tag="ACHUQ7_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFR1134495.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRAVFLKQPEKSNAAYWRATHKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(26491..27828)
                     /locus_tag="ACHUQ7_000977"
     CDS             complement(26491..27828)
                     /locus_tag="ACHUQ7_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1134496.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(27903..29051)
                     /gene="dgoD"
                     /locus_tag="ACHUQ7_000978"
                     /pseudo
     CDS             complement(27903..29051)
                     /gene="dgoD"
                     /locus_tag="ACHUQ7_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(29048..29665)
                     /gene="dgoA"
                     /locus_tag="ACHUQ7_000979"
     CDS             complement(29048..29665)
                     /gene="dgoA"
                     /locus_tag="ACHUQ7_000979"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFR1134497.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(29649..30527)
                     /gene="dgoK"
                     /locus_tag="ACHUQ7_000980"
     CDS             complement(29649..30527)
                     /gene="dgoK"
                     /locus_tag="ACHUQ7_000980"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFR1134498.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(30524..31213)
                     /gene="dgoR"
                     /locus_tag="ACHUQ7_000981"
     CDS             complement(30524..31213)
                     /gene="dgoR"
                     /locus_tag="ACHUQ7_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HFR1134499.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLAAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            31491..32147
                     /gene="yidX"
                     /locus_tag="ACHUQ7_000982"
     CDS             31491..32147
                     /gene="yidX"
                     /locus_tag="ACHUQ7_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidX"
                     /protein_id="HFR1134500.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(32193..33005)
                     /gene="yidA"
                     /locus_tag="ACHUQ7_000983"
     CDS             complement(32193..33005)
                     /gene="yidA"
                     /locus_tag="ACHUQ7_000983"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFR1134501.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(33119..33517)
                     /locus_tag="ACHUQ7_000984"
     CDS             complement(33119..33517)
                     /locus_tag="ACHUQ7_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFR1134502.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(33757..36171)
                     /gene="gyrB"
                     /locus_tag="ACHUQ7_000985"
     CDS             complement(33757..36171)
                     /gene="gyrB"
                     /locus_tag="ACHUQ7_000985"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFR1134503.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(36200..37273)
                     /gene="recF"
                     /locus_tag="ACHUQ7_000986"
     CDS             complement(36200..37273)
                     /gene="recF"
                     /locus_tag="ACHUQ7_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFR1134504.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMDDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(37273..38373)
                     /gene="dnaN"
                     /locus_tag="ACHUQ7_000987"
     CDS             complement(37273..38373)
                     /gene="dnaN"
                     /locus_tag="ACHUQ7_000987"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFR1134505.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(38378..39781)
                     /gene="dnaA"
                     /locus_tag="ACHUQ7_000988"
     CDS             complement(38378..39781)
                     /gene="dnaA"
                     /locus_tag="ACHUQ7_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFR1134506.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAACQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            40078..40158
                     /locus_tag="ACHUQ7_000989"
     CDS             40078..40158
                     /locus_tag="ACHUQ7_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1134507.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            40388..40528
                     /gene="rpmH"
                     /locus_tag="ACHUQ7_000990"
     CDS             40388..40528
                     /gene="rpmH"
                     /locus_tag="ACHUQ7_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFR1134508.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            40545..40904
                     /gene="rnpA"
                     /locus_tag="ACHUQ7_000991"
     CDS             40545..40904
                     /gene="rnpA"
                     /locus_tag="ACHUQ7_000991"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFR1134509.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            40868..41125
                     /gene="yidD"
                     /locus_tag="ACHUQ7_000992"
     CDS             40868..41125
                     /gene="yidD"
                     /locus_tag="ACHUQ7_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFR1134510.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            41128..42774
                     /gene="yidC"
                     /locus_tag="ACHUQ7_000993"
     CDS             41128..42774
                     /gene="yidC"
                     /locus_tag="ACHUQ7_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFR1134511.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIVAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            42880..44244
                     /gene="mnmE"
                     /locus_tag="ACHUQ7_000994"
     CDS             42880..44244
                     /gene="mnmE"
                     /locus_tag="ACHUQ7_000994"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFR1134512.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT        10787 a        12039 c        11354 g        10255 t
ORIGIN      
        1 tctttatgat aaataataat caaattgata aaatcaaaat gagaaaaata tgaataattc
       61 cattaaccat aaatttcatc acattagccg ggctgaatac caggaattgt tagccgtttc
      121 ccgtggcgac gctgttgccg attatattat tgataatgtc tctattctcg acctgatcaa
      181 tgccggagaa atttccggcc caattgtgat taaaggacgt tacattgccg gtgttggcgc
      241 agaatacgct gatactccgg ctttgcagcg gattgatgct cgcggcgcaa cggcggtgcc
      301 agggtttatt gatgctcacc tgcatattga atccagcatg atgacgccgg tcacttttga
      361 aaccgctacc ctgccgcgcg gcctgacgac cgttatttgc gaccctcatg aaatcgtcaa
      421 cgtgatggga gaagccggat tcgcctggtt tgcccgctgt gccgaacagg caaggcaaaa
      481 ccagtactta caggtcagct cttgcgtgcc cgctctggaa ggctgcgatg ttaacggtgc
      541 cagttttacc cttgaacaga tgctcgcctg gcgggaccat ccgcaggtta ccggccttgc
      601 agaaatgatg gactaccctg gcgtaattaa cgggcagaat gcgctgctcg ataaactcga
      661 tgcatttcgc tacctgacgc tggacggtca ctgtccgggt ttgggtggca aagaacttaa
      721 cgcctatatt gctgcgggta ttgaaaactg ccacgaaagt tatcagctgg aagaaggacg
      781 ccggaaatta caactcggca tgtcgttgat gatccgcgaa gggtccgctg ccagcaatct
      841 caacgcactg gcaccgttga tcaacgaatt taacagcccg caatgcatgc tctgtaccga
      901 tgaccgtaac ccgtgggaga tcgcccatga agggcatatc gatgccttaa ttcgccgcct
      961 gatcgaacaa cacaatgtgc cgctgcatgt ggcatatcgc gtcgccagct ggtcgacggc
     1021 gcgccacttt ggtctgaatc acctcggctt actggcaccc ggcaagcagg ccgatatcgt
     1081 cctgttgagc gatgcgcgta aggtcacggt gcagcaggta ctggtgaaag gcgaaccgat
     1141 tgatgcgcaa accttacagg cggaagagtc ggcgagactg gcgctgtctg ccccgccata
     1201 tggcaatacc attgcccgcc agccagtttc cgccagcgac tttgccctgc aattcacgcc
     1261 cggaaaacgc tatcgggtca ttgacgtcat ccataacgaa ttgattacgc actcccgctc
     1321 cagcgtctac agcgaaaatg gttttgatcg cgatgatgtg tgctttattg ccgtacttga
     1381 gcgttacggg caacggctgg ctccggcctg tggtttgctc ggcggctttg gcctgaatga
     1441 aggcgcgctg gctgcgacag tcagccatga cagccataat attgtggtga tcggtcgcag
     1501 tgccgaagag atggcgctgg cggtcaatca ggtgattcag gatggcggcg ggctgtgcgt
     1561 ggtacgtaac ggccaagtac aaagtcatct gccgttaccc attgccgggc tgatgagcac
     1621 cgacacggcg cagtcgctgg cggaacaaat tgacgccttg aaagccgccg cccgtgaatg
     1681 cggtccgtta cccgatgagc cgtttattca gatggcgttt ctttctctgc cagtgatccc
     1741 cgcgctaaaa ctaaccagtc aggggctatt tgatggcgag aagtttgcct tcactacgct
     1801 ggaagtcacg gaataataaa aaaagcccgg cgtcatgccg ggcaaaagtc accagttacg
     1861 tttatgccac tgtcaactgc tgaatttttt tctcgcggcg gattttgcgt tcttccatta
     1921 ccgccactat cgccatcagg cagatacaac caatcgcggc gatatccagc gcggcgaagg
     1981 tgcctgccca gccggtaagg ccgaataccg gcgtcccatc ggcaatcatt cccagaccta
     2041 acttggcaaa gctgtcacca atcaggtaag caaaggtgcc tttaatacca tcggcagcgc
     2101 caatcgcttt tttaggtaca aagccaacag cagccacacc aatcaacaat tgcgggccaa
     2161 agaccaggaa acccaacgca aagagagaag ccagatagat atattcgtta ctggcatgtt
     2221 gatacacacc gagcgtggcg ataatcagcg ccagcgcgat gcaggccacc aggccacggc
     2281 gaccgttcgc caggtcagag agccagcccc acagcagcgt accgaccagc gcaccagctt
     2341 caaacagcgt aaagccctga atcgccaccg ctttagagag tttcagttcc tggaacgcgt
     2401 atacggttga ccactggtcg ataccaatac gtaccacata gaggaaaatg ttggcgaagc
     2461 acagcagcca gatcactttg tttttcagca catactcaac aaagatctgc cacttggtca
     2521 tatcggtaga ttctgtctct ttgtcctctt cgctgatctc ctcgccgaac agttcttcag
     2581 ctttgccgag gccataagat tccggggagt cgctgccgta acgcaggccg ataaaaccga
     2641 caatcagcgc gataatcgac gggaagataa acatgccgat gacatggcca tcgaacaggt
     2701 aatttgcccc gaacagcgcc acacctgctg cgcctgcacc gccaaggttg tgagaaatat
     2761 tccagaaacc gaggaatgtc ccgcgtttac gacgcggcgt ccatttggtg atggtggagt
     2821 aactgcacga accgccggta ctctggaaaa agccgcttaa ggcgtagaag gcaatcatca
     2881 ggaacaggct aaccgagccg ctgcccatac tggcactgaa gcccagcata caaatggcag
     2941 agaggataag catgaacggc aggaattgtt tggtgttttt gccgtcggcg tagtatgaaa
     3001 ccaacgtttt acccacgcca taagtgatgg agaaacccag gccgatcatc cccagctgcg
     3061 tcatgctcaa cccgtaggtc gaaatcatat cgttctgcgc gatgttaaag ttcttgcgaa
     3121 tcaggtacat cgtcaggtag ccgataaaga ccaccaggta ggattgcatg aacggtttga
     3181 accacatttt gcgccgcact tcgagcggaa ggtccagggt cggcttgcga acttggttta
     3241 ggaaagccag catgggttac tcctgaaatg aatacctgcg tgaggcatgc attgtaaaaa
     3301 tcagcagcaa atcaggcctg agatagcgtc caagaaaaac ctggaaaatt tcttagtttt
     3361 gccggatatg aagtgaaaag gtgagatgca tcacgcttca cgcggtgtct gggcgttcaa
     3421 aaagggcagt aacagcagtg cggaaatccc ggcggcgata gagataacca caaaaaatcc
     3481 gctccagtgc caggtatcga gcactttcgc cagcggccaa ccagcaagcg acgcccccag
     3541 ataagcaaac aagccgacaa accccgtcgc cgcccctgcc gcctctttgt gggaacactc
     3601 tgccgccgcc ataccaatta acatctgcgg gccaaagaca aaaaaaccaa tggtgaagaa
     3661 gcaggttgcc tgcatcacgt agctggcaaa tggcatcagc cacagcgagc cgactgaaag
     3721 caaaattccg gcggcgaaaa tcaaattcat cggcccacgg ttgccgttaa acaatttgtc
     3781 cgagccccaa ccggctacca gcgcaccgat aaatccgccc agttcaaaca tcgtcactgc
     3841 cgtattcgcc gtgaccagat cgacgcccag cgtctcggac atatacaaat tgccccagtc
     3901 gttgatcgcc gcccggacca catagaccag cacatagcaa aacgaaagca gccagatata
     3961 cggattcagc aacacatatt tggtgaggat ctctttacgc gtcaaccctg ccccttcttg
     4021 ttgttgagca atttccagcg cgtcgtgccg ccattcaccg accgccggta aacctaacgc
     4081 ctgcgggcga tcgcgtagcc gccagcagag aaaaatcccc acgactatcg ccatacaacc
     4141 agcaatcatc atcccggcac gccagccgta atgcagcgcg ctcgctgcca tcacaatggg
     4201 aatgagtgcg ccgccgacgt tatgcgccgt gttccataat gcccaccaac cgccgcgctc
     4261 ggtacgtgaa taccaggccg ttaacagacg cgcacacacc ggtgaacccc agccctggaa
     4321 aaaggcgttc agcacccaga gcacggcaaa cgcccatagc gacgtcgaga agccaaacag
     4381 aatgttcatg atgcccgtgg cgataagccc tatccccata aaataacggg catttgagcg
     4441 atcgctgaca atgccggaga caaacttcga cacgccgtaa gtaatgtaaa acagggtcgc
     4501 taacaggccg atatcgctac ggctgagcac gccgttagca aggatttctg gtaccgcggc
     4561 gttaaaactt ttccgcgtga agtaaaacag cgcgtaaccc agccagatgg tcagcagaat
     4621 atgccgacgc cagtagcgat aacgggcatc aatttcatgt ttatcagtca ttaatggcgc
     4681 atcggcaggc gctttcagaa acggcaacat catcgaacct tagacataac gttgaggtag
     4741 agaaacgctg acacgcgtgc cgtgcagaca ggaaatgtgt aatgtgccgc ccagcgccgt
     4801 tacgcgctcg cgcattccgg tgaggccaaa accttgttgc ccggaacccg gcggcaaacc
     4861 gctgccatcg tcttcaatca ccagcatcaa ccgttcatcc tgctgccagc cttgcagcgt
     4921 aaccgcgctg gcatcggcat gtttcacaat gttgttcagc ccttcctggc agacacgaaa
     4981 cagcgtcacg cgctggtttt cgcttaatgc tgattcatcg atacgccatt cgagatggct
     5041 gacaatgccg cgcccttcca gctccatttc ccgcatcagt gagcggatgg cctgctccag
     5101 ggtgagatca tccagctggc gcggacgtaa ccgtcctaac aaacggcgca ccgcgtcgta
     5161 aacgcccagc gacagttgtt cgatgagctg cccgctctgc ttcacactgg cgttatctgc
     5221 cgccagccgc tgaacaatgc ccgcctgagt acgaatagca gtgatggtct gaccgatatc
     5281 gtcgtgcagc tcacgcgcca cgtcgcggcg cacgctctct tcggtttcta acaaacgttc
     5341 agccagatgc tgattgcgcg ccagttcctt ttgtagcgac tggttaagtt cacgcaaccg
     5401 ctggatgcct gcgcccagta acaaccctgt cagactttgc accagcaacg agagtaataa
     5461 atccaccaga tgatcgcgcc aggtttgact ggcgatcagc gcgatggcgt tcatcaacgt
     5521 cgcaatcagc gctccttgcc agccatagtg ccaggccagc gcgataatcg gcagcgccag
     5581 acagaatggc gtaaagcgcg acagttcgtc cggcaatccc aactggagcc agagactgat
     5641 aacaaacagc agcaagtacc agaccagatg ccgcccgcgc cagttgatgg gctgagaaac
     5701 cagtgacggg ccgagcggca gccaggtgtt attggcgaga tagtgccaga acaccagaca
     5761 tatcggggcc agcgtcagac cgccagttaa agtcagcaac agcgcattcc acgactcttt
     5821 gccgtgccaa agccagggca gcgactgcaa caacgccgcc gccgttaacg ccgcaccctg
     5881 taacagcaag gtgcgccagt cacgctgatg gcgatagcgc gagatcagcg ctacgggcag
     5941 taacgtcagc aaactaccga tcatcaataa cggaaaatgg gttaaaccga ccgcctgcgt
     6001 tagccagtaa atcagcagcc actccgcacc cagcaatacg ggccagtatc cgcgcgggca
     6061 ttgcagcatt agccccagac gcagaccaaa cggaaataac agcaccgcca tatcagggcg
     6121 ctcaaccaga tgcaggctga tactccatag gcaaaaccat gcggcagaga agataaaaaa
     6181 gcaggcaata acggtaatta agcgggagaa caacgtcttc atcaccagcc atcaaacatg
     6241 cggcgcgcca gctctacgtc gttactgacg cccagttttt ccatcagatt ggcgcgatgg
     6301 acgtgtaccg ttttcggtga caagcccagt tcggcggcaa tctctttcac cgccattcct
     6361 tgcgccagtt tttccgccac ctggcgttca cgtttggtca gcgggtcctg acgaccggat
     6421 gccagtttaa tggcaatatc cggcgtcaga taacagccgc ccgtggcaac cgtatgcacc
     6481 gcagcaatga gttcatccgg gctacagcgt ttggaaagaa agccgcgtgc ccccgcgtta
     6541 agcgcctgct caaccagcgc aggactgtcg tgaacggaga gcataatcgt cgccatacct
     6601 ttcggcagct ggcttagcag ctccagaccg gagatatcgg gcatggagat atcgcaaata
     6661 cacacctgca caccgcgccc cggcagcccc gccagcgcct cgcgccccga accaaactcg
     6721 gcaactacct gcaaatcagg ttccagcccc agcagctgcg caaagccgga gcggacgatg
     6781 aggtgatcgt ctataagggc aacggtgatc atggtcttgt cctggcgggt aaaaaaaaac
     6841 gcgcttacct taacgataag cgcgatgttg ttcaagcctt gagcggttac tgaaaaaaca
     6901 ccgcgatctt gttaaacatc gtcggatcgg actgattacg ctgcactttc acgacatctt
     6961 ccagcttagc gatttggctt atcatctgct ccagacgctg gtcgtcattg accagtagcc
     7021 agatatggct tttgtcgctg tcctgaatcg gcagacaaag aatgccttca acgttaaaag
     7081 cgcggcgggc aaaaaggcca caaacgtggg tcattacgcc cggatggttg cgaacggtga
     7141 gctccagaat tacgttgtta tgagttgtgt tttgcatggc ttattccccc accatttcag
     7201 tattcgccgc acctggcggc accatcgggt aaactttttc ttcggcatca atgcgcacat
     7261 ggatcagcgc cgggccaggg cgattgatga tttcctgcaa tgcagcctgc ggatcggctt
     7321 cgttattcaa atcacaggtt tcgaggccga atccggcggc aatctgcata aagttgattt
     7381 tgcccggata ggtggcggca aaaacgcctt gctcgtagaa cagactctgt tgctgatgca
     7441 ccagccccag cgcttcgttg ttcatcagaa tgattttgac atccagctga ttttcactgg
     7501 cggtcgccat ctcctgaata ttcatcatca ggctgccgtc gccggagaaa cacaacactt
     7561 tgcgatccgg gttcgccagc gccgcgccaa tcgccgcagg caggccaaaa cccatcgtgc
     7621 ccagcccacc ggaggtcagc cactggcgtg ggcgattgag cggataagct tgcgcggtcc
     7681 acatctgatg ctgacccaca tcggtggtga taatcgcatt gtcatcgaca caggcggcaa
     7741 cggcgttgat caggccgtaa tggcttaatg gatcgcacgc tttcgggatt ggacacggga
     7801 attcgcgctg caaatccgct accaactggt gccactctgc acgcggttgc gcttccaccc
     7861 gcgggatcaa ttgcgccagc acgtcatcaa catccgcctg aatcgccaca tgcggctgct
     7921 tgattttacc cagctctgcg cgatcgatat cgacatgaat aattttggca ttcggacaga
     7981 actgctcggt tttgccaatc gcccggtcat caaaacgcgc accgagcaca atcaacaaat
     8041 ccgcctcctg caaaatatag ttggtactgc gcacgccgtg catccccagc atacccagcg
     8101 acaacggatg cgcttttggc agcatgccca gcgccattaa agtcatggtg gtaggcagtt
     8161 gcgctttctc cgccagttca cgcacccgcg caggcgcatt gatcacacca ccacccagat
     8221 aaagcaccgg gcgtttggca gcgttaatca ttgtagctgc gtcacgaatg ctttcttcgc
     8281 taaaggcggg tgcaacggct ttttctgcca ccgcgggctg agcttcaatc tcaaaaaccg
     8341 ccgtttgtac atccttagga atgtctatcc acaccgggct agggcggcct gattgcgcaa
     8401 tgcggaaggc atcgctcatg acctgtggga gctcttcgat atgtctgacc agatagttgt
     8461 gtttggtgat ggggatagag atgccgtagg tgtccacttc ttggaaggcg tcggtgccga
     8521 tcatcgaggc gggaacctga ccagtgatgc aaatcagcgg gatggagtcc agccgcgcat
     8581 cagcaatggc ggtcaccagg ttagtcgcac ccggcccgct acaggccata cagaccgccg
     8641 gtttaccgtc ggtgcgcgcc attccctgag cgataaaccc cgcgccctgt tcatggcgag
     8701 ccagaatatg gcggatttgc gtgctttggc ttaaggcgtc gtaaacaggc aggatagaac
     8761 cgcccggaat gcccgtcaca atcttaatgc cctgctgttc caggaaatga acgataaatt
     8821 ctgcgccggt aaagcgctta cgcgtcgatg ttgtgcccga acttgccatg ctccagtcct
     8881 tttcttctgg gccgactttc cgggaggtgc ttaaacgaaa aaccccgccc ggtttgcgcc
     8941 ggcggggttt tggaatcgtg tgttgttcca gtccctacgg cgcattgccg acgaccacca
     9001 ccacacgcac gacgaccact gcggcggatg gcgcagttgg tagtagtttt gcgttgaaca
     9061 tggaagtagt cattggggac cttgttggtt ttgtgtttaa caatatttat acaagcacag
     9121 ctttacaggg gagacaatgg aaaatttttc agcaagggaa aattgagggg ttgatcacgt
     9181 tttgtactga attgcagata acaaaaaacc ccgccggagc gaggtttcgt cagccgcctg
     9241 cggctggtaa ccgcaaagca cactgtatta tgtcaacact gaaagtatac gtgttccgcg
     9301 cagaacgcgc aatttcggca cgaattttga cgtatttagt gcatagttga gtatcgttca
     9361 cagtttgcgt tttgtccaaa tattactgtt tatttataca gtaagcttct ataatatcac
     9421 tgtacgcaat gtgttatgcg ggggccgcat cgttacccgg cgcactaagt cctggctgaa
     9481 acgggtggtg ccgtcagcgc cttaaccccg cgtgagcaca ctgtgttatg tcaacaagca
     9541 caacgtttct ccttgagata ccgcgtgcac aacagctggc aacaggcagc ggaaaggtac
     9601 gtcagctggc agtgctcctg aaccacagga gacgcgtatg agcctggtgg atatcgccat
     9661 tcttatcctc aaactcattg ttgcagcact gcaactgctt gatgctgttc tgaaatacct
     9721 gaagtaattc agattcaagt cgcaccaaag gggagcgggt aaccgctccc cttttatatt
     9781 tagcgtgcgg gttggagtcg gatgcgatgc tgacgcatct tatccgccct accatccctc
     9841 ccggcaacat ttattgccgc ttttgtttac atattctgcc gctaaacaat tccccattcc
     9901 tggcgtatat ctggctaaca ttcatcaatg tgatagattc ctctcccgca tttatgggaa
     9961 tgcgtagtga cttattctaa ttatttttat aaaagcatcc gtgataatga aaaggcaaag
    10021 aaacgtcaat ttgttattga tgttggtatt actcgtggcc gtcggtcaga tggcgcaaac
    10081 catttatatt ccagctattg ccgatatggc gcgcgatctc aacgtccgtg aaggggcggt
    10141 gcagagcgta atgggcgctt atctgctgac ttacggtgtc tcacagctgt tttatggccc
    10201 gatatccgac cgtgtcggtc gccgaccagt gatcctcgtc ggaatgtcca tttttatgct
    10261 ggcaacgctg gtcgcggtca cgacctccag tttgacggta ttgattgccg ccagcgcgat
    10321 gcaggggatg ggcaccggcg ttggcggcgt aatggcgcgt actttgccgc gtgatttata
    10381 tgaacggaca cagttgcgcc atgctaacag cctgttaaac atggggattc tcgtcagtcc
    10441 gttgctcgca ccgctaatcg gcggtctgct ggatacgatg tggaactggc gcgcctgtta
    10501 tctctttttg ttggttcttt gtgctggtgt gaccttcagt atggcccgct ggatgccgga
    10561 aacgcgtccg gtcgatgcac cgcgcacgcg cctgcttacc agttataaaa cgcttttcgg
    10621 taacagcggt tttaactgtt atttgctgat gctgattggc ggtctggccg ggattgctgc
    10681 ctttgaagcc tgctccggcg tgctgatggg cgcggtgtta gggctgagca gtatgacggt
    10741 cagtattttg tttattctgc cgattccggc ggcatttttt ggggcatggt ttgccgggcg
    10801 tcctaataaa cgcttctcaa cgttgatgtg gcagtcggtt atctgctgcc tgctggctgg
    10861 cttgctgatg tggatccccg actggtttgg cgtgatgaat gtctggacgc tgctcgttcc
    10921 cgccgcgctg ttctttttcg gtgccgggat gctgtttccg ctggcgacca gcggcgcgat
    10981 ggagccgttc cccttcctgg cgggcacggc tggtgcgctg gtcggcggtc tgcaaaacat
    11041 tggttccggc gtgctggcgt cgctctctgc gatgttgccg caaaccggtc agggcagcct
    11101 ggggttgttg atgaccttaa tgggattgtt gatcgtgctg tgctggctgc cgctggcgac
    11161 gcggatgtcg catcaggggc agcccgttta agcgcacgtc accgcagcat cgtcatcagc
    11221 tccatgggag aacgatgctg ctttatcaga tcacgcatca cccgcatatg cggtgcggag
    11281 taagaataaa acgcctgata gcccgcacaa agcacgctgt ctggcgtctc ctgccgagcg
    11341 tgcgggcaca ggcgatgcca gtcgcacgcc tggcagtcac ggcgcggttg cgggttttcg
    11401 ctgccacacc atttttgtaa ggtggcgtca aaaagctcaa tcgagatacg actgatatct
    11461 gcccgcaccc aacactcaaa cagggcaatt aaaaatttcc cccacagctc tgctgtcacc
    11521 gaagcggccg tcagatcgcc ccggtcatca aattcaagat agggaatgag ctgtacggag
    11581 cggatgcccc gctgtttgaa attgtgataa aaatcggcag gttgcagagc atccttgtac
    11641 tctacaacca gcttatgcct gtcctcttca gcatcaatga cctgacttcc tgtcatgcga
    11701 ctctctcaat aaatacgata agttgatgta tatgcgttac agcaaacagt attgcgagag
    11761 ataacaccgc gagggagatc aaaaatttgt cacggactac gtgaaattgg gaaaaatcgc
    11821 tattcgtgac atccattaaa ttgcgattac gggtgtagtg ccagaggatc agcgccacga
    11881 tggcgaggat gccaatcgaa atccagaata acatgcccgc ctggtgccag ttgtgtttga
    11941 tagccaacgc catcaacgcg ccgtagccca gcatggtacg aaaccacgcc agtgatgtgc
    12001 gctccggctg cagccctggg tcggcaatgc gtctggcttt acggctatcc ggcatacaac
    12061 accagtccca taacgatcac cgccacaacc atcaatatta agctgatgat taataagctg
    12121 ttggtgtagg gcaggtcctc tttcagacgc atcgcttttt cattgcgtaa ccagcgcagg
    12181 tagccataca ttgctaaccc gccggaaaac agacacaaca acaacgccag cagttcacga
    12241 atgacgggcg tggcgaaatc tggcgcaagt tgatcaagcc caacccccgc cgccagaaat
    12301 cctagtgcgg tacggatcca cgccagaaaa gtacgctcat tagccagcga gaagcggtaa
    12361 tcgggcgctt ctccaaggcg ggaaattttc attgttgctc ctttgcaatt tcactaacgc
    12421 tctatcttta gcggtttttt ttaagaattt cgtccgcccg tttcctgata tgcttttgcc
    12481 atccgctaca aggaggtcag atgggtatca tagcgcaaaa taaaatcagt tcattgggca
    12541 tgctgttcgg tgccatcgct ttaatgatgg ggattatcca cttttcattc ggcccttttt
    12601 ccgcaccacc gccaacgctt gaaagcatcg tggcggacaa aacggctgaa attaaaagag
    12661 gtcttctcgc cggaataaaa ggcgaaaaaa ttaccactgt ggagaaaaaa gaggacatgg
    12721 atatcgataa aatcctcgat caaagcggta tcgcactggc cattgctgcg ctactttgcg
    12781 catttattgg cggtatgcgt aaggaaaatc gctggggcat tcgcggcgcg ctggtttttg
    12841 gtatcggcat cgtgtgcagc attttgctga tattcctgat tttttccttc ctgaccggcg
    12901 gttcgctggt ttaagcagcc tgaataatgc ccgccgggtg atcatcagcg ggcattatcg
    12961 tcagaatttc tgttttttct cctccacctt cactccttgc gtcggtaggc cggtgtcata
    13021 gtcgcgtacc actggcgaat agccatcttg tgggcgtggg cgaaacgccc ccatccagcg
    13081 cggtcgtgca tctttacgcc acggacgcag actccattgg taactgcgga acggatcgcg
    13141 aattttgtcc atgtaatcca ataaggcgtc atgcattttg ctgcgaacgt ctgcaaaacg
    13201 gatatcatcg atcaggttat gcatttcatt tgggtcatta cggcgatcgt aaagttcatc
    13261 actggtgaag aggttgagca ccagtttaaa gtcatccgtc acccagcaac gcaccggaat
    13321 aaaaccgcca aagctgtcat gctcaatctc gtagcggtta aattccacca tcacgccgcg
    13381 tggctctttc acggcaagga tattttcccc cggcagaatc tctggttttt caatatctgc
    13441 cagcgccatc attgtcggca gtaaatcaat atgactgact ggcgtatcga cctgtcgccg
    13501 ctccccttgc ggcgaacgga tgatcagcgg aatacgggtg atgtcgtcat acatcgccgc
    13561 tcctttactg atcagcttat gtgcgcccat catttcaccg tgatcggagg tataaatgac
    13621 ccacgtattt tcacgttgct ctggcgttaa ggcattgatg acacgtccga tttggtcatc
    13681 aacaaagtca ttgcaggcaa aatagagcgg atggtgataa agcccgtcat caccgactgg
    13741 cgatggcatc gcctgcgccc ataagcggtg atgttccggt ttgttcgcca ggtcatcctg
    13801 tgatttctcg ccaagttcgt agtaaaaatc agcgtatttc tctaaatact ccaccggaca
    13861 ggtgaacggg tgatgcggct catcatacga aaccaccatc aggaagggtt cctcggcgcg
    13921 cacgggctat tgcagaaaat ctaccgctcg attgctgatg cggtgcgccc aggtgaaggt
    13981 ttcgtcaata tggttcgcct gtaaatcttc gacgctgttt aggccattgc gccacaggct
    14041 gatctctttt tccgtcagtt cgctaagata gttcgcccca tcaaaccagt aatcagcatc
    14101 ccactccggc ggacactcgc cagtgccgaa atagtcatga ccgtcgagat gccatttgcc
    14161 gatgtaacag gtgtgatagc cggcatcctt aaagtagcgc cccatagtgg agatgttttt
    14221 gcctggcgcg acgttgttgg tccacgggcc ggactggtta gcgtagatac cggtgaatag
    14281 tccggcccgt gcaggcgtac aaaccggtga acaagtgtag gcggaattaa agcgaatacc
    14341 ttccgccgcc agactatcaa tattttgcgt attcagcggt ttaccgctat agcaaccgac
    14401 catattggtg gcctgggtat cggtcaattg gggcgtttca taggctatcc ttcgcgtcgg
    14461 gagtaaaggt tacggctggg tcgtgacgat gccaccagct gtcaaaaatc aggtagacaa
    14521 tcactacggc agcgatgaaa taactgatca tcgctaacca gcgcgtaccg tagccgccga
    14581 attcagccag cccgacatag acgccaatca tcgcgaacag gataccaatt gacgcgatct
    14641 tgacgttttt ccacggtttc atgtccaccg caaacgcatc tttgaaggtg aacggcgtgg
    14701 cgcgcggttt gataaaaccg atcaccagca tcacgaccac gttgatgcag aacgtacagg
    14761 ccagcacata gaggaaatgg aagtcgaact tcaccagata gttgatggtg atgtagctga
    14821 ttatgccaat ccccatcgcc acttttgccg ccagcgccgg gatgcgcgga aagaaaaagc
    14881 ccataatgat gatggtaacc agcggcacgt tgtaaatgcc gttgagctgt ttcatccagc
    14941 tatacagccc ctgcggcgcg ttcgcgatcc acggcgctac caaaaccgaa acgatagcga
    15001 taaagaaacc aaatttgcgc ccgacggtga ccagctgctg cggctcggca ttctggttaa
    15061 tgatgcgacg gtaaataccc atactgaata atgtactggc gctattcaga aagccgttga
    15121 aggtactgat caccgcacca aataacaccg cgccgaagaa accctccagt ggcactggca
    15181 gaacgttatt gaccagcgtc gggtaggcca tgtcggcttt cggtagatcc tgatacagat
    15241 gaaacgcaat caaccctggc agcaccagta ccagcgggtc gagcattttc agcaccgccg
    15301 ttaacagcgc ccccttttgc ccttccgcca ggctttttga cgccagcgtg cgttgcacga
    15361 tgccctgatt tgtacaccag taaaaggtgt tcaccagaat taaaccggta aatgccgcgc
    15421 caatcggcaa gggatcggtc gggccaccga ctgagtttaa tttctcggcg tgaacggtgg
    15481 taatttgctc aatgccctgc ataaagctgc ccttgcctat cgcgatcagg ccaaataccg
    15541 gcaccatcaa cccgccgata accagcccaa taccgttgat ggagtccgcc actgccattg
    15601 cgcgcagtcc gccgatcacc gcatacaaaa ttcccgccag accaagcaaa attaccagta
    15661 gccagatagc cgcaccgtga gaaatctgta gcgattcccc gacgtgaaac aggctgttga
    15721 gcgccaacgc gccggagtag agaacaatcg gcagaaagca gacgccggtg gcaatgagga
    15781 agcagaagtc gatgataata cgcgtcgttt tatcataacg ttcctccaga aaatcgggga
    15841 tggtggcaat gccgcgcttc agatagcgcg gtaaaaagat tagcgcgagg aagatcagcg
    15901 tcaccgctga agtcacttcc cagcccatca ccgacatgcc gcttttgtag gcctgcccgg
    15961 aaagaccaac cagttgttcc gtggaaaggt tggttaacat taacgaagcg gcaataaccg
    16021 gcgcttttag tgaacgtccg gcaagaaaat agccttgttg cgatccggta tctgttttgc
    16081 gaaccttcca ccaggtgatc accgccacca gcagcgtaaa accgacaaaa ctcaagattt
    16141 gtaacgaatt catctcatag cccttattta tgtttatgtc ccggacgcat gaatataggg
    16201 agaggtaaaa tgcgtcgggt caggcagtca gattcttaaa atacaaacgt cgtatccctg
    16261 aacggattgt tttctgttaa gttcaggttg tgagcataat tcgccggagg gatattaaaa
    16321 tgaatggaaa attgcaaagt tcagatgtaa aaaacgaaac tccgtacaat attcccttat
    16381 tgattaacga aaatgtgatc tccagcggaa tttctctgat ctcgctctgg catacctacg
    16441 ccgacgagca ttaccgggtg atctggccgc gggacaagaa gaaaccgctt atcgccaact
    16501 catgggttgc ggtgtatacc gtacaaggat gtgggaaaat tcttttaaag aatggcgaac
    16561 aaataactct gcatagcaac tgtattatat ttttaaagcc aatggatatt cactcttatc
    16621 actgtgaagg tttagtctgg gaacagtact ggatggaatt tacccccacc agtatgatgg
    16681 atatttccgt tggtcagcaa agcgttattt ataatggcga aatttataat caggaactca
    16741 ccgaagttgc tgagttaata acttcaccag aagcaataaa aaataatctg gcagtcgctt
    16801 ttctgacgaa aattatttat cagtggattt gtcttatgtc cgcagatggt aaaaaagatc
    16861 cacaacggcg gcaaattgaa aaattaattg ccactttaca tgccagtctg caacaacgct
    16921 ggagcgtagc tgatatggct gccacgatcc cctgtagcga agcctggttg cgtcgtctgt
    16981 ttttacgcta taccggcaag acgccgaaag aatattacct cgatgcgcgt ctggatctgg
    17041 cgctatcgct attaaagcaa caaggaaact cggttggcga agtcgctgat acgctcaact
    17101 tcttcgactc ctttcatttc agcaaagcct ttaaacataa atttggttat gcgccgtcag
    17161 ccgtgctaaa gaatacggac cagcacccaa cggatgccag tccacacaat taacgtaact
    17221 ctggccagta cgctttgttg gcctccagca actcatccag aatagctttg gcgaccgagg
    17281 cgctcggtac cgttttcgac aacgtaatcg cctgccacag gtgctgatat gaacgctgtt
    17341 cccaggcgtc cacgaccagt ttttccaccg ccacttgctg actcattaac cctttctgaa
    17401 actgcgggat atcgccgacc accagtggtt caggccgtag tagcgcacgc gcatctgttt
    17461 gcgatcttgc agcccaacaa tttgcgccat ccggctttca ataccgattg gcatgtcaca
    17521 gatgttgagg attttcgcat tcgggcgcag acgacgcgtg gcttctgcga caattgctgc
    17581 cgggttggag tagttgagca tctaggcgtt tggtgaatat ttttccatat aatccaccag
    17641 ttccaggacg ccgccaatgg aacgcatgcc gtacgctatt ccgccgggtc cgcaagtttc
    17701 ctgaccaaca acaccgtggc gcagtgggat tttttcatcc agttcgcgca tcgggtactt
    17761 gccgacgcgg atgtgcgcca tgacaaaatc aacgtcgctg aacgccactt caggatcggt
    17821 ggtgtaacta aacgcaatgt ccggcgcttt ttctttaagg atgactttac aggcttcggc
    17881 aatcacttcc tgccgcgcgc catcgttatc ataaaatttc agtgcacgaa gcgggaaacg
    17941 gtcctgattc gccaggagca tcaacacgat gcctggcgta aaggtgcttc caccgcctgc
    18001 gacaaccact gagaatttgg tcattatact gcctccgtaa tggcaacatt ttctgctgat
    18061 tgatgagaat taattaagct atcgagctgt tcacgcaatt gggatacatg cagaccaatg
    18121 atcacctgaa tggcatcgcc actacggaag acgccgtgcg ctcccagctt tttaaagact
    18181 tcgtcatcca gcgtttgtga catgtcatgc agtgcaatac gtagacgcgt cgcgcaattg
    18241 ttaatgctgg agatattgcc gaccccgccc agggcttgca ggataccggc agcctgatcc
    18301 agctcttttt ttggctctgc agcggtggtt tggcctcgcg aggctttgta ttcggctttt
    18361 gagtagagtt tcacttccgc atcttcacgt cccggcgtgc acatgttgaa ctgcagaatc
    18421 agggtgcgga aaaccacgaa gtacagcagg gtaaagcaca acccaatggc gatttgggtc
    18481 agcatcatat ccgcgtggtt gctgaacatc gggatccagt tttgcggtaa aacctggtca
    18541 atcagacctc cgcccatgtt gcccaccaca ccaaagagat acattacggt cgacattgag
    18601 gccgccagca cggcgtgtac cgcaaacagc aacggtgaaa tgaacaggaa ggtaaattcc
    18661 agcggttcgg taattcccac cagcatggcg gttaaggttg cgggaatcag caagcccgcc
    18721 acttttaccc gattttccgg tgcggcagtg aagtacatcg ctaaagaaat gcccacggca
    18781 ccaaagattt ttgagttacc gtgcagggca aaaccgcctt ccgggaacaa cgatttcagt
    18841 ggctcggcgc tcagactgaa ctcttgcaga tgctgcgccc agtacatctg aatgccgcct
    18901 tcaacagctg ccggaccaaa gataaacggt ccgtagatga agtggtgtaa accggttggg
    18961 atcagaatac gttcgaggaa ggtgtaaacc cacaccccaa gcgcacccgc cgaacgcagg
    19021 aacgcttgca gagattcaat ccccatttgt acttttggcc agccgagcaa tgtcagccag
    19081 gcacagggga tcatcaccag gaaggcgata atcaccacat aagacgtccc ctggaaaatg
    19141 ccgagaaaaa ccggcagttt tttatcgaac agacggttat gcagcgccgt cacaatgccg
    19201 gaaatgataa ttgcgccgat aatgctggta tcgagggttt taatcccggc catcattgtc
    19261 agaccgctac ctgccaccgc gtcctgagtg aaatcgacgc cgaagtagct tccccaggtc
    19321 attcccatcg cattgatgaa atagttccag gtcaggaaac tcaccatcac cgccagacaa
    19381 gcacgtccct gcgcttgctt agcaaggcca atgggtaaac cgacagcaaa aatcagcggc
    19441 atattacgga ataccgtcca accgccctct tcaataatgt gtacgatttg cgcgaataaa
    19501 ctgttcggat cggtcagtga ttccccgaca aacatcgggt tttgcagcaa gatggcaaga
    19561 cccaccacaa tcccggcgaa gggaaacagc agcactggcg tgaacatcgc gccgccaaag
    19621 cgttgaattt gactgagcat ttccacatcc tcttttctca attctgtaag taggaagcct
    19681 gattggttat gcggcagacc tggagcgagg ataggaagat gcgatcggca gaacatgacg
    19741 acccaatggg attcgtgatc gctttcatgc ttttcgttgg gcggtagaga tctacgtttt
    19801 cgcgaaaaag tagacatctg tgagcacagg tacacagcat cgaacacatc tttaaaaaaa
    19861 agattttttt caatcgatta agcagaactt gtgggcgcat tacccgtgct tgcaggcaaa
    19921 aaagagagct agagatgatc tacaaaagca ttgcggagcg gttaagaatt cgacttaatt
    19981 ccgcggattt cacgctaaac agccttcttc ccggtgaaaa aaagctggcg gaagagtttg
    20041 cggtatcgcg gatgactatc cgtaaagcca ttgacctgct ggtagcgtgg gggctggtgg
    20101 tccgccgcca cggcagcggc acttacctgg tgcgcaaaga tgtgctgcat caaaccgcca
    20161 gcctgaccgg actggtggag gtgttaaaac ggcagggaaa aacggtcacc agccaggtgc
    20221 tgatttttga aatcatgcct gcgcctccgg ccattgccag ccagttacgg attcaaatca
    20281 acgagcagat ctacttctcc cgtcgcgttc gttttgtgga agggaaaccg ctaatgctgg
    20341 aagacagcta tatgccggta aaactgttcc gtaacctttc gctgcaacat ctggaagggt
    20401 cgaagtttga atatattgaa caagagtgcg ggattttgat tggcggtaat tatgaaagcc
    20461 tgatgccggt gctcgccgat agactgctgg cgcggcaaat gaaggtagcg gaacacacgc
    20521 cactgctgcg gatcacctcg ttgtcatata gcgagagcgg ggagtttttg aattattcag
    20581 tgatgttcag aaatgccagc gaatactagg tggagtacca tttacggcga ctccacccgg
    20641 aaaaaagtta accgatactc cagaagagca ccgccagtaa ttggggggtg ataatgcgca
    20701 ggaacatcac taacggatag acagtggcgt aagagagcgc cgccgcacca ctggttggat
    20761 gaagattatt agcaaacgcc agcgccggag gatcggtcat ggaacctgcc agcatcccgc
    20821 acatggtcag gtaattcatt ttggctaaca tccgcgccag aatgccaaca gtaatcagcg
    20881 gaacggcggt gatcagggca ccataaccaa tccagcttag cccttcgcca ttgaccaggg
    20941 tattcacaaa atccccacca gatttcagac caacgaccga gaggaacagc acgatcccca
    21001 gctcccgcag cgcgaggttg gcacttggcg gcataaacca gtacagcttg ccgatactgc
    21061 cgatacgccc gaggatcaac gccataatca gcggaccgcc cgccagcccc agtttcaacg
    21121 cggccgggaa tcctggcaca aagacgggaa tagaacctaa caatacgcca agcccgatgc
    21181 caataaacac tggcagcatc tgaacctgtt gcagtttttg ctgcgcattc cccagcacat
    21241 tggcaacggc atcaattgcg gacggacgcc ccaccaggtt gaggatatcg ccgaactgca
    21301 ggctgatatc gccactggcg accagttcga ccccggcacg gttcaggcgc gagataacaa
    21361 cgtcatagcg ttctttaaag tgcaggtcgc gaatacgttt tccgagcacg ttttcattgg
    21421 tgaccaccac acgctcgacg cgcaaatcag tgcctttcgt agacagcgag gtatcgacct
    21481 cctgaccaat caccagttgc gcattatgta aatccgctgg ctgccccacc agatgcagca
    21541 aatcgcccag ttggataatg gtatctggcg aaggtacttt tagggtttct tctcgtttca
    21601 gacgcgagca gataattttg tcgccgttga gaatcggtac atctttaatg gctaaatcat
    21661 gcaggttagg gttctcaacg cgaatattga tagtcttgat cagcgcgccg ccattggtgc
    21721 gtgaagactc gtgctgctga gcttctgtct cgacattgac gcggaaaata acccgcaaca
    21781 tccacatggt gaacaaaatc ccgcaaatgc cgaatggata cgccatcgcg taactcatcc
    21841 ccatctgatc gaccatttcc attggtgtac ccaggtcgcg caagatctgc tgccctgccc
    21901 ccagcgctgg cgtattggtt accgcaccgg agaaaatccc cagcactacc ggcagtggaa
    21961 tatcaaacag tttatgcagg atggcggtaa ccagaccacc gatgatgacg atcagaacag
    22021 caaacaggtt gaggcgtaat ccggagacgc gcaatgaggc aaagaagccc ggccctacct
    22081 gaatcccgat ggtataaacg aacaggatca ggccaaattc ctgaataaca tgcagcatat
    22141 cgctacttaa tgtcatcccc gcctgagaaa caaaatggcc gacgatgatc ccaccaaaca
    22201 gcacgccgcc aatacctaat cctatgccgc gaaatttgac gttgccgata aacaaaccga
    22261 cgactgccac caaagccaga atactgaccg ttaatgctat atcactcatc gtccatttct
    22321 cttgagataa cattttaatt gctacggatt atggctgaat cggtgaagca gatttgcctt
    22381 atagcgcaca ttatcacggg aaagaacgtg ccgaaatatc ttaaacagtc gaaatttaac
    22441 gcattgttga agaagaaaga caaaaaaagc cccaccagaa tggcggggca aagagaatag
    22501 ctagttagct atttaacgcg ggacgttcgc tgatagcgat acgctgcgct gcgatgggtt
    22561 caggctcatt acgaattaaa tcaatatgca gtaaaccgtt tacgaaggtt gcgccagaga
    22621 cttccatatt ttcagccagc gtaaaactca ggctaaatgg ctgattcata agcccttgat
    22681 gcagccattt tttctcttct tttggctgct ccggcgtgcc ttttacgctc aggcgcgtgc
    22741 cttccagttg aatctctaaa tcttcctgac ggaaacctgc cagcgcaagg gtaatgcggt
    22801 agtggttatc gtcgcttttc tcaatgttgt acggcgggaa gctctggctt tcaccggcgt
    22861 tttgcagtgc gttggccagt ttgtcaaaac cgatccattg acgcatcagt ggggataaat
    22921 cgaagttacg catagtcatt tctccttcta agaagcgagt aagtacctgc aaatccgaag
    22981 attcgcatat gctccctgac ggcgagcatg gagatgtcag gccgcgccag gcggccttag
    23041 ggaattagtt gatttcgata cggcgcggtt ttttcgcttc cggaatcacg cgttcgagat
    23101 cgatatacag caaaccattt accaggttag ccccacgaac atgaatgttc tcagctaact
    23161 ggaatttgcg ttcaaagttg cgttcagcga tgccctggta cagataggtg cgctcttttt
    23221 gttcgtcggc gtgagcacct ttcaccacca gcagattatc ctgggcggta atttccagtt
    23281 cgctctcagc aaaaccagcc acagcgatag caatgcggta atggttttcg tctaccagtt
    23341 caacgttata cggagggtag ccgccattac tctggctctg gttgttttct aagtggttaa
    23401 acaaacggtc aaatccaata gcagaacggt aaagcgggga taaatcaaag ttacgcataa
    23461 tcaatagctc ctgaaatcag cgagaatgta agaccttcca caatggacag gtcaggtagc
    23521 cagaacaccc atcaggcgcg tcctcatcga ctacgatgta aaatgggtct ggaaatgaac
    23581 ttttcaagcc ttatcagcgg actttttttt gcagtttatg gtctattgca tagactgagg
    23641 gggcagcacg aaacgttaaa gtgcgatagc cgccacagtg cgacgtaatg atgatgttat
    23701 tttcagcaag ggatcgctat aactcatcag gccaacaccc acgatcaaca gatgaattta
    23761 ttatgataag aaatgtgttg ttagcgttca tgatatgcag cggaatgaca ttactcggag
    23821 gatgctccag cgtgatgtcc cataccgggg ggaaagaagg aacgtatccc ggcacccgcg
    23881 ccagcgccac catgattggc gacgatgaaa ccaactgggg cactaagtcg ctggcgattc
    23941 tcgatatgcc gtttaccgct gtaatggaca cgcttttact gccgtgggat gtgttccgca
    24001 aagacagctc ggtgcgctcg cgcgttgaaa aaagcgaagc caacgcgcaa gccaccaatg
    24061 cggttattcc accggccaga atgcctgata actgatttag cgatcagttt cggtgatcca
    24121 cagctggcca ccttccatcc acgccagcca ttgaccatcc ggcgagaaga ccacggcgtc
    24181 cgcagaaggt ggattggcgt ggttttcggt taaatactca acctcgccgc tttgcgcatg
    24241 ggcacaaaca attcgattat ccagcacaaa gcccaaccat tctcctgacg gatgccagtt
    24301 aaatgcagac tgaatatccg ttttgttatg ggttaacttg cgcggttcgc cgccctgtgg
    24361 cgagataagc cacagttgca caatgccgtt atcatcacgc attaaaaacg cgatttgcgt
    24421 accctgcgga ttacagcgca cccagtggcg cgggacgttg actaaccccg gataagcccg
    24481 atgatgggta aaggttaaac gtcgctgcac gacgccacgc ggtggcgcgg gtagggttgt
    24541 ttccgttcca cttaacggcg catcacctgc cgatttccag ctagcttcat cttgcggtaa
    24601 ctcaacgata aatagctccg gcactttctc gccctttggc gaaagtgtgt cgccaataaa
    24661 cgccagcgcg tgatttccta cccatccttc ttcataagca cgattgattt catcactgcc
    24721 aggctgcggc gtgggcgtgg ttttactcac cagcacacac cagtggctac cgctgtattc
    24781 acgcggatgt tgcttttgta cgttgaccgg gccaaacggt gcagcaaccc cgacgtttcg
    24841 caaatccagc gccggatcga gttgatgcat tacatggtcg ttataggtaa agctccccct
    24901 ttcaccgttc gggctaaaga catgcacatg gctgccaccg cgcagcgctc ctggcgtata
    24961 cggcgcggta atatccattg catcgagatt gctcaccttg ccgccttcag caatgactcc
    25021 gcgtcgatga tggaagtcgt aatgccatgt ttcatcagga ttttcagggc cgtgaataaa
    25081 gacatatttc tctgacttcg gatgaacggt caccacgccg acgtgtgcgc cctgtgacgc
    25141 gcgatagata acctcgacct cgcctgtatg gatattcaca cgctcaatgg tctcgccggt
    25201 aaacgacgcc ccagaaggac gcacgtcaaa taccagccac tggctgtcgg gcgtccaggt
    25261 attggtattt gtattggtat tggtattggt gagcaggtga ttacggggag caaaggttat
    25321 ctgtttcatg gcgcggtcct gatacaaaac ggttcccgcc atcatacttc acaaggactt
    25381 cactttcaga caatagtctt ttgccatcgt gatgaatgtg agtgaaaaga tggaacattt
    25441 cgacgtggcg attattggcc tcggcccggc agggtcggcg ttggcacgaa agttagccgg
    25501 caaaatgcag gtgatcgcgc tggataaaaa gcaccagtgc ggtactgaag gtttcagcaa
    25561 accttgcggc ggtctgctgg caccggacgc gcagcgatct tttattcgcg atggactgac
    25621 gcttcctgtc gatgtgatcg ccaatccaca gattttcagc gtcaaaactg tcgacgtcgc
    25681 cgcatcgctc acgcgtaact accagcgaag ctatatcaat attaatcgcc atgctttcga
    25741 cttgtggatg aaatcgctga tccccgccag cgttgaggtt tatcacgata gcctgtgccg
    25801 aaaaatctgg cgtgaggatg ataaatggca tgtcattttt cgtgcagacg gctgggagca
    25861 acatattacc gcccgctatc tggtcggtgc cgatggcgca aactcgatgg tgcggcgaca
    25921 tctctacccg gatcatcaaa tccgtaaata tgtcgctatc cagcagtggt tcgcagagaa
    25981 acatccggtg ccgttctact cctgcatctt tgataatgcg ataactgact gttactcatg
    26041 gagtatcagc aaagatggtt attttatctt tggcggtgcc tatccaatga aagacggtca
    26101 gacgcgtttc acgacgctga aagagaaaat gagcgccttt cagttccagt ttggcaaagc
    26161 ggtgaaaagt gagaaatgca cggtgttgtt tccctcacgc tggcaggatt ttgtctgtgg
    26221 taaggacaac gcctttctga ttggtgaagc ggcgggattt atcagcgcca gctcgctgga
    26281 ggggattagc tatgcgctgg atagcgcaga gattctgcgt gcggtgttcc tgaaacagcc
    26341 ggagaagagc aacgccgctt actggcgcgc cacacacaaa ctgcgtttaa aactcttcgg
    26401 caagatagta aaaagccgat gcctgaccgc accggcttta agaaagtgga ttatgcgcag
    26461 tggtgtggcg catattccac agttgaaaga ttatccaacg cgcttcacat cacccaccag
    26521 caggatgtaa gagagcgcgc caatcaacgc gacggcggag atataaacca gtgcaggcgc
    26581 gaaaccgtaa ccctgcgcca ggtaccccac caccagcgga acggtgatgc cgcccagacc
    26641 accggcgaag ttaaacacgc cgccggttaa accaatcagg cgcatcggtg ccagagaaga
    26701 gaccagcgac caggtaatcg aagcaaaacc gttgccgaag aatgccagcg ccatcaggca
    26761 cataatcatc atcggatcgt tggtgtagtt agcgcccata atgcaggtgg agatcagcaa
    26821 gccgcagata atcggcgttt tacgcgcaaa gcccagtgaa aagcccttac gtaccagcag
    26881 atccgcgacc cagccagaga gcaggacgcc gacaaacgcc gcgaggaatg gcaccgtggt
    26941 cataaagcct gctttcagcg ccgtgattcc tttttcctgg gttaaatagt tcgggaacca
    27001 ggttaagaaa aaccacagtg cagaagccac cgcaaattgc ccaagataaa cgccgatcag
    27061 tttacgatgg aacaccagtt tccagtcttt ggctgttaac ggctgacgcg cctctttctt
    27121 caccggcgca tcaccatcca ccagaccgcc gccatcacga atgtaatcca gttcagcttt
    27181 gctgatacct ttggtcaggc gcggcggctg ataaacctta aaccaaatca gcgaccagat
    27241 aatgccgata ccaccagtga caatgaacac ccagtgccag ctcaacatct cctgaatcca
    27301 gatcagcagc ggagtcaaaa acgccagacc gacaaactga ccagacgtat aaaaaccaac
    27361 ggcggaagcg cgttcatgtt ccgggaacca gctggtcacc atccggttat tggtcgggaa
    27421 agcgggcgct tcgaaaatac cggttatcgc acgcagacca attaatgaca ttaatcccgt
    27481 ggcaaagcct tggaataaag tcgccactga ccaaccaaat atcgcaataa aataagtcac
    27541 gcgagaacct acgcgatcta aaaaccaacc gccggggatc tggcatagcg tataaagcca
    27601 ggcgaaggcc gaaaatacat agcccatttc cgctttcgta atgccgaact cttcctgaat
    27661 atgggcggaa gccacggcca ggttagcgcg gtcaacataa caaatgacca ccgtaataaa
    27721 gatcatcacc agcgtcagat aacgccgacg ccccggcttt gctgcattaa cgggaatatc
    27781 catagcgagc tttctccaga ttttgggcat agcgaagccg ctcaccatgc cctgtaattt
    27841 acagagggtt atatttttgt attgctgttt tagtgcccga tgaggggctt acgtggcagg
    27901 aattaccact ctgctacgct gttatcttca tgacgccaga gcggattacg ccagtccggg
    27961 gcatttttac tgaactcaat aactttagct tcgtcgattt ccacacctaa gcccggtttc
    28021 gttaacggtt taaagaagcc gccaaccatg ctgaagtctt ctttgttttt cacaaagtcg
    28081 agtaactccg cgcctttgtt gtaatgaatg cccatacttt gttactgaag taccgcgtta
    28141 taggaaacga agtcgatatg caggcaagcc gccagtgcaa tcggtccgaa cggacagtgc
    28201 ggcgcaaggg tcacatcata ggcttctgcc attccagcaa ttttgtagca ttcggtaata
    28261 ccgcctgcat gggagagatc cggttgcaga atcgaaatac cgcctgcctc cagcacgcgt
    28321 ttaaaatcga aacgtgagaa catgcgctca cccgccgcca gtggaatatg cgtttgtgcc
    28381 gccaatttcg ggtagtattc ggcttgttcc gccagcaccg gctcttcaat aaacagcggg
    28441 cgatacggct ccagttcttt aatcagcact ttcgccattg gcgcgctgac gcgaccgtgg
    28501 aaatcaagac caaactcaat ctgattgcca aaagcttcac gaatttgtgc cacggtgttg
    28561 actgccgcat ctaccgcgcg ggagttatca attagcccca gttcttcaca accgttcagt
    28621 ttgaaggtat cgaagccgat ttcgcgcagg gttttaatgc cgtcgataac atccgccgga
    28681 cgatcgccgc cgacccaact gtaggcttta attttgtcgc gaaccaggcc gcccatcagt
    28741 tgccagactg gcgcattcag cactttgcct ttgatatccc ataacgcctg gtcgatcccg
    28801 gcgatggcgc tcatcaggat tggaccgcca cgataaaatc cggcgcgata catcacttgc
    28861 cataagtcgt tgatgcgcga aggatcctga ccaatcaaat agtcacccag ctcgtgaact
    28921 gccgcttcca ccgtacgggc gcggccttca atcacgggct cgccccaacc gaccacgcct
    28981 tcatcggttt caattttcag gaacatccag cggggaggta aacgatacgt ggtaattttg
    29041 gtgattttca ttgcactgcc tctcgatacg ccttaacaaa tgctgctgcc tgctgcgcgg
    29101 tgcgttctac ggactgcccg gcgcgataaa gatcgctgcc taagcccgcc ccaacacaac
    29161 ctgcgtctat ccactgcgcc aggttttctg gcgtcacgcc gccaacggca aagactgcga
    29221 tgtccggtgg taataccgct tttaacgctt tgatgtattg cggaccaaaa gccgatgacg
    29281 gaaatatttt cagcgcctgc gcgcccgctt cgagcgcggt aaaggcttcg gtcgccgtcg
    29341 cgcagccggg gcaaacggtc atgccgtagc ccaccgcgcg gcggatcact tcactatgga
    29401 tattgggcgt aacgatgagc tgacagccca tcctggcgag cgcatcgacc tgttcaggtt
    29461 tcagtaccgt acctgcgcca atcaacgcct tgtcgccata cgcatcaacg atggcgggaa
    29521 tgctctgctc ccattgtggg gaattcagcg ggatttcaac cgcgtcgaac ccggcgtcaa
    29581 tcaccgcgcc aacatgcgcc agcgcctcgt cgggcgtaat gccgcgcaaa atggcgatca
    29641 gcgggagttt agtttgccac tgcatgagcg atgctcctta taccagcctg aaatgccgtg
    29701 tcgcccgcca ccgccgccac gtcgcaaccc atcgcctgaa aggcttgctg gtagcgcgcg
    29761 gtcagcgatg ttccggcgac aagggtgatg gcgtgttgat gggtcacata gtcgcgcata
    29821 ctggcaacct ctgcgccaat caacaaacca gagagaaatt cgctgacctg ttcgcgggga
    29881 agtgttccca gcacatgcga ggcgcgaact tcaaaaagct gcggcaatat ggcgggcgca
    29941 ttaaggccgc gctcaaggcc agccgcgaag gcatcggcag agttttcctg cggcggcaaa
    30001 cctgcgccaa tcaatgagtg atttaacagt aaatgatgta attcaccggt catcacggtg
    30061 cgaaaatcgt tgatttgctg gctatcggcc tgcacccatt tgcaatgcgt accgggcata
    30121 acataaagag aggaaggagc cagagctcgc gcgccgatca attgtgtttc ttcgccgcgc
    30181 atcacattgt ggttatcgtc atgagagacg cataatccgg gaataatcca gatattgtcg
    30241 ccaacagacg ttaattgttc gccaatagac gaaaaacggg caggaacaga taaatacgga
    30301 gcaactttcc agccgacatt gctgccgacc attcctgcca tcaccactgg cgtattttct
    30361 tcacgccagt cggtcgtgac ttctgctaac actgcagccg gagattttcc gttcaggcgc
    30421 gtgacgcctg cttctgattg cctgctctcc aggcagtggt cgccctgata aagccaggcg
    30481 cgcagattgg tcgatcccca gtcaattgcg atgtagcgag ctgtcatgtg atttccttta
    30541 accttcgtgt cgagctggca atcatggtaa gcgccgcctg ctctgccgca tcgccgtcct
    30601 gatgccgtat cgcatcgaac agcgccttat gttcctggag cgtttgcggc atgttggcct
    30661 catcgcccat ccaggttcgt tcaaaaaccg cccgctgcag cgaactgatc gcaatgctaa
    30721 gttgctgtaa caccgggtta tgcaccgact gcagcaccgc ctcgtggtag cgaatatccg
    30781 cttcgttaaa cgcttcgcgg tcctgattgt tggcaatcat ctcgttcagc gccgattcaa
    30841 tctgcgccag atcgctggag gtcgcgcgct ctgctgccca acgggcaatc gccggttcca
    30901 ccagatttcg cacttcgctc atggcactga taagccgtgg gtcgtagtcg ttttccagca
    30961 cccattgcag tacgtcagtg tcgaggtaat tccactggtt acgcggtgcc acaaacgccc
    31021 cgcgataacg tttcatttca atcagccgct tcgccatcag cgaacggaac acctcacgga
    31081 tgatgttgcg cgaggttgca aactcttcac agagctccgc ctcagccgca agtggcgagc
    31141 caggtacgta tttgccgtga acgatctgtt tacccagcgt aatgacaatg cgatcggttt
    31201 tattgagagt catggagagt ccttgtgctc tgtatgttct tctctacttt accccgatcg
    31261 atgcataacg cggcaacttt gtagtaccag cgtgatgacg ttcgcgtttg ccgcgcgcgt
    31321 aatgtagtac aaacttatat tgttgcgcta caatttagat caaaaaaaga acaacacata
    31381 aaaaaatgac atgcgtcggg cagaaatctg aaaagggata tcaggcgcta aacaggaggg
    31441 aaagaagagt atgctttcaa cggcttagct actcgtttaa aggattaatc atgaagttga
    31501 attttaaggg attttttaag gctgccggtt tattcccact ggcgctgatg ctttcaggct
    31561 gtatctcgta tgctctggtt tcccataccg caaagggtag ttcaggaaag tatcaatcgc
    31621 agtcagacac catcactggg ctatcgcagg caaaagatag taatggaaca aaaggctatg
    31681 tttttgtagg ggaatcgctg gattacctta tcactgatgg tgccgatgac atcgttaaga
    31741 tgctcaatga tccagcactt aaccggcaca atattcaggt tgccgatgac gcaagatttg
    31801 ttttaaatgc ggggaaaaag aaatttaccg gcacaatatc gctttactac tactggaata
    31861 acgaagaaga aaaggcactg gcaacgcatt atggttttgc ctgtggtgtt caacactgta
    31921 ccaggtcact ggaaaaccta aaaggcacaa tccatgagaa aaataaaaac atggattact
    31981 caaaggtgat ggcgttctac catccattta aagtgcgatt ttatgaatac tattcaccca
    32041 gaggcattcc ggatggtgtt tccgcagcat tactgccagt gactgttaca ctggacatca
    32101 ttactgcacc gctgcaattt ctggttgtat atgcagtaaa ccaataatca gtaagcgggc
    32161 aaacgcgttt atgctgtttg cccgcccaca gattaattca gcacatactt ctcaatagca
    32221 aacgccacgc catcttcaag gttggatttt gtgacaaagt tcgccacttc tttcaccgaa
    32281 ggaatagcgt tatccatcgc cacaccgacg cctgcatatt caatcattgc gatgtcgttt
    32341 tcctgatcgc caatcgccat gatttcttcc ggtttaatac ctaacacgtc ggccagtgat
    32401 ttcacacccg tacctttgtt aacgcgttta tcgaggattt cgaggaagta cggcgcactt
    32461 ttcagcacgg tatatttctc tttcacttcc tgcggaatac gcgcgatagc ctggtcgagg
    32521 atggcgggtt catcaatcat catcactttc aggaactggg tattggggtc cattttctcc
    32581 gcttcgcaga acaccagcgg aattgtggca acgaaggatt catgcaccgt gtagtagctg
    32641 atatcgcggt tagcggtgta cagcgtggtg cggtccaggg cgtggaaatg agaaccgact
    32701 tcgcgagaga gtttttccag gaaacgatag tcgtcatagc tgagagcggt ttgcgccacg
    32761 gtgctaccat cagcggcctt ctgtaccagc gcgccgttat aagtaatgca gtagtcgccc
    32821 ggctgttcca tatgcagctc tttcaggtag ttgtgcacac ctgcatacgg acgacccgtc
    32881 gttagcacga cattcacgcc acgggcgcga gctgcggcaa tcgcattttt aacggcgggt
    32941 gaaatggtgt gatcgggcag cagaagggtg ccatccatat cgatagcaat gagtttgata
    33001 gccatgagtt cccaaggtag attagttcct gacccatgct aacgcgattc cgctcaaaaa
    33061 tcagtacaac acccgaggga aaaggggatg caacgcgggt tgcatgctcc ccatttgctt
    33121 agcggaagag tttccctttc agcagttcca tgcctgcgga aagcagatcg ttattggctt
    33181 gtgctgacac ttcaccttgc ggtgagagcg catcaataat cttcggcaat tgctctgcca
    33241 gtaaactgga agccgtactg gtatccacgc caagtttttg cccgagatcg gacaccgcat
    33301 ttgtgccgag cgccgactcc agttgctcgc cgctaactga ttgatttctc tgttgattac
    33361 tcaaccaggt tgagagaatg gcccctaagc cgccactttg cagtttttcc agcagcacct
    33421 gaatgccacc ctgctcctca acccaactta aaatcgcctg atatttcccc gcatcgcctt
    33481 tcagaaaggc accgacaact tcatcaaaaa gccccatgat aatcacctgt aaagcgttac
    33541 gtgttgaccc aaaaagtata gatctgcggg atgataattg cggattgcag aaataaaaag
    33601 ggcggagatg atctccgccc ttttcttata gcttcttgcc ggatgcggcg tgaacgcctt
    33661 acccggccta caaaatcatg caaaatcaat atattgcagg agtctcgtag gactaataag
    33721 cgtagcgcat caggcacgct cgcatggtta acgccattaa atatcaatat tcgccgcttt
    33781 cagggcgttc tcttcaataa acgcacgacg cggttctacg gcgtcgccca tcagcgtggt
    33841 gaacaactgg tcagcggcaa tcgcatcctt aacggtaacg cgcagcatac gacggctttc
    33901 cgggtccatg gtggtttccc acagctgttc cgggttcatc tcgcccagac ctttataacg
    33961 ctggatggag aggccacgac gggactcttt caccagccag tccagcgcct gctcgaagct
    34021 ggctaccggc tgacgacgct cgccacgttc gataaacgca tcttcttcca gcaagccacg
    34081 cagtttctca cccagcgtgc agatacgacg atattcgcca ccggtgataa actcgtgatc
    34141 cagcggatag tcagtatcca caccgtgggt acgcacgcga acaatcggct cgaacaggtt
    34201 ttgttcggca ttggtgtgga catcaaactt ccactggctg ccgtgctgtt ctttgtcgtt
    34261 cagttcgctg accagcgcgt tcacccagcg ggtaacggtc tgctcatcag aaaggtcagc
    34321 ttccgtcaac gtcggctgat agataagctc tttcagcatt gctttcggat aacgacgctc
    34381 catgcggttg atcattttct gcgtcgcgtt gtactcagat accagtttct ctaacgcttc
    34441 gccagccaat gccggtgcac tggcgttggt gtgcagcgtt gcgccgtcca gcgcgataga
    34501 gatctggtac tgatccatcg cttcgtcgtc tttaatgtac tgttcctgct tgcctttctt
    34561 cactttgtac agcggcggct gagcgatgta gacgtgaccg cgttcaacga tttccggcat
    34621 ctgacgatag aagaaggtca acagcagcgt acgaatgtgc gagccgtcga cgtccgcatc
    34681 ggtcatgatg atgatgctgt gataacgcag tttgtccggg ttgtactcgt cacgaccgat
    34741 accacagcca agcgcggtga taagcgtcgc cacttcctga gaagagagca tcttatcgaa
    34801 gcgcgctttc tcgacgttaa ggattttacc cttcagcggc agaatcgcct ggttcttgcg
    34861 gttacgcccc tgcttcgcag agccgcccgc ggagtcccct tccaccaggt acagttcgga
    34921 aagcgccgga tcgcgttcct ggcagtctgc cagtttgccc ggcaggccag ctaagtcgag
    34981 cgcaccttta cggcgggtca tttcacgcgc gcgacgcgcc gcttcacggg cacgggcagc
    35041 atcgataatt ttgccaacca cgattttcgc atcagttggg ttttccagca ggtattccgc
    35101 cagcagttcg ttcatctgct gttcaaccgc cgatttcacc tcagaagaaa ccagtttgtc
    35161 tttggtctgg gaggagaatt tcgggtccgg cacttttacg gaaacgaccg caatcaggcc
    35221 ttcacgcgca tcgtcaccgg tggcgctgac tttggctttt ttgctgtagc cttctttgtc
    35281 catgtaggca ttcagggtac gggtcatcgc cgcacggaag cctgccaggt gagtaccgcc
    35341 gtcacgctgc ggaatgttgt tggtaaagca gtagatgttt tcctggaagc catcgttcca
    35401 ctgcagcgcc acttcaacgc caataccgtc tttttcggtg gagaagtaga agatatttgg
    35461 gtggatcggc gttttgttct tgttcagata ttcaacgaac gctttgatgc cgccttcata
    35521 gtggaagtgg tcttctttgc cgtcacgctt gtcgcgcaga cgaatggaaa cgccggagtt
    35581 gaggaacgac aactcacgca gacgtttcgc cagaatttca tattcgaact cggtcacatt
    35641 ggtgaaggtt tcgaggctgg gccagaaacg caccatggtg ccggtttttt cagtctcgcc
    35701 ggtaaccgcc agcggagcct gcggtacacc gtgttcgtag atctgacggt gaattttacc
    35761 ctcgcgctga ataaccagct ccagtttttg cgacagggcg tttactaccg aaacaccaac
    35821 gccgtgcaga ccgccggaca ctttatagga gttatcgtca aatttaccgc ctgcgtgcag
    35881 aacggtcatg atcacttccg ccgccgatac gccctcttcc gggtgaatac cggtcggaat
    35941 gccgcgcccg tcatcctgta cagagacaga gttatcggcg tgaatggtga cgataatttc
    36001 tttacagtga cccgcgagcg cttcgtcgat agcgttatct accacctcga ataccatgtg
    36061 gtgcagaccg gtgccgtcat ccgtgtcgcc gatatacata cccgggcgct tacgcaccgc
    36121 atccagccct ttcaggactt tgatactgga ggagtcataa gaattcgaca tcaacgtttc
    36181 tcgctcattt atacttgggt taatccgtta ttttaccctt ttccacggta aacatcttcg
    36241 aattttcgtc cgacatgtct ataacgtgtt cagcactgat cgcgctgaca aagacctgtg
    36301 attgcgtcgc ttttaagcgg ctggcaagca acccgcgacg ctcatcatca agctcagagg
    36361 caaaatcatc tatcaggtag agacaccgcc gcccgctttc acgagtgagg aactctcctt
    36421 gcgccagacg taaggcgcac atcaacagct taagctgccc acgcgataag gtatcttcca
    36481 ccggcgcacc gtcggcgcga atgcgtaagt ccgctttatg cgggccatgc gcggtgtagg
    36541 ttagctggcg atcgcgttca aaattacgtt ccagcacctc agcgtactct gtctctttct
    36601 cccagccgcg ctggaaagag aaagtcagag aaaactcagg gagaaattgc ttacaggtat
    36661 catccatatc ggccgcgata ccggcgctat actccgcgcg ccaggtgctg atttgctccg
    36721 ccagcgggat cagttcttta tcccacgggc gtagctgttc gtaacgagtc acctggcgca
    36781 gcgccgcatt gcgctgcttg agcaatcgct tgagattgct ccaggcggtg aaaaatccag
    36841 gttcgttgtg aaagcatccc cagtcgagga atgctcttct gtatttgggg ccgccgttga
    36901 gtaaagtaaa cccttctggc gttatcaact gcatcggcat caggtgcgcc agttccgcga
    36961 ctttatgccc gtccgtaccg tcaatacgga ctttgctgtc gccctgtttg tctttggtta
    37021 agccaatcgc cgtctcgcgc tcttcgccct gtaatcgccc gtggagaaca aatgcctcct
    37081 gctcatggcg aatgacgcga ccaatctgca aactgcgaaa cgcccgacca tggccgagcg
    37141 tatagatggc ttccagcacg ctggttttgc cactgccgtt ggcacctacc agaaagttaa
    37201 agccgggaga taaagcgaga tccgcggttt caatattgcg gaaatcgcgg atcaacaagc
    37261 gggtgaggga cattacagtc tcattggcat gacaacatag gcagccgact gtgatgcggc
    37321 atcttcaatc tgcacgctgg aaaccgaatc ggtcagcatc atgcggacgt tttcgcattt
    37381 cagcgcgttc agaacatcca gcacatagct gacgttgaag ccgatttcca tctccgcacc
    37441 gctataggta acgtcgagga tctcttccgc ttcttcctgt tccgggttgt tggcagtaat
    37501 tttcagctgg ttttcgctga catacaggcg cacaccgcgg aatttctcgt tagagagaat
    37561 cgccgcgcga gcaaacgcct gcttgagcag atcgcagcct gcttccagat gtttgtccgg
    37621 gttcttcggc agaacgcgac ggtaatccgg gaagcgacca tccaccagtt tcgaggtgaa
    37681 aataaagtcg ccaacgtggg cacgaatgtt attgctgcca atctgcacac gcagcagatt
    37741 gtcgccgccg tcgagcatac gcatcagttc aatcacgcct ttacgcggta cgatcaccga
    37801 atggcttggc aaagattgac caattggcat tgaacagacc gccagacggt ggccgtcggt
    37861 tgccacggtg cgcagttctt caccttcggt ttcaaacagc ataccgttta agtaatagcg
    37921 aacatcctga tgcgccatcg aaaactgggt tgcttcaatc agacgcttca tcgtcgcctg
    37981 cggcagggta aattcgactt cgctctgcca gtcatcgagg ttcgggaaat ccgccgctgg
    38041 cagggtagac agcgaaaaac ggctacgccc ggagcgcacc agcatccgtt caccttccag
    38101 ctgcacggca atttccgcgc cttcaggcag accacggcag atatcaaaga atttgcgcgc
    38161 cggaacggtc gtcgctcctg gctcgtgtgg ctgaaccagc gcaacgcgtg ccaccatttc
    38221 catctcgaga tcagtaccgg tcagcgacaa cgtaccgtca gcaacctgta acagcagatt
    38281 accgagaatc ggtagcgtag gacgaccacc taacggaccg ctcacctgtt gtagcggttt
    38341 taataaatgc tcacgttcta cggtaaattt cataggttta cgatgacaat gttctgatta
    38401 aatttgaaaa atcttctttg atatcgtggc tctcttcacg caactgctcg atcttacggc
    38461 aggcatgaag caccgtcgtg tggtcacgcc caccaaacgc atcgccaatc tctggcagac
    38521 tgtggttagt cagctctttc gctagcgcca tcgccatctg gcgcggacgc gccaccgagc
    38581 gggatcgacg cttggaaagg agatccgcga ctttgatctt gtagtactcc gccaccgtct
    38641 tctgaatatt gtcgatggtg accagttttt cctgcaatgc cagcaagtcg cgcagcgcct
    38701 cacgcacgaa gtcgatggtg atcgcccgtc cggtaaagtt ggcgttagca ataacgcggt
    38761 tcagcgcccc ttccagctca cgtacgttag atcgtagacg cttggcgata aagaacgcca
    38821 cttcacccgg caaacgaatg tcattttcgt cggccttttt catcaggatc gccacacggg
    38881 tttccagctc tggcggttcg atcgccacag tcagtcccca accgaagcgg gatttcaaac
    38941 gatcctcaac gccgttgatc tctttcggat agcgatccga ggtgagaatg atctgttgat
    39001 taccttccag cagggcgttg aaggtgtgga aaaactcttc ctgagatcgt tctttattag
    39061 caaaaaactg aatatcgtcg atcagcagtg catctacgga acggtagtag cgtttaaact
    39121 cttcgatcgc gttgttttgc agggctttaa ccatgtcctg aacaaagcgc tcggagtgca
    39181 tataaaccac tttggcattc ggcttgcgcg ccataatgcc gttacccacc gcatgcagca
    39241 ggtgagtttt acccagaccc gtgccgccat aaaggaacaa cgggttatag gcaccgccag
    39301 ggttatccgc cacctggcaa gccgccgcgc gcgccagttg gttagattta ccttcaacga
    39361 agttatcaaa cgtgtgtttg acgtttacgt tagaacgata ggtcggttct gccggggccg
    39421 ggacgttatc ccaacctgag cgcgtagaag gcgcagcacg ttgcggctgc gtttgcgcca
    39481 cctgtgcagg ggccgcgacg ttgctcgtca ctgccgcttg tggcgtttgc gtcaccggtt
    39541 tggtgccgac ttcaaaacgc agctgtgggg catccgctcc gcagaaactg gttagcagtc
    39601 cattgatatt attaaggtac ttgtcccgta cccaatcgag gacaaaacgg tttggcgcgt
    39661 acagggccag cgtgttatcg ctcagttccg cctgcaatgg gcgtatccac atactgaatt
    39721 ctgtggctgg taactcatcc tgcaatcggg caagacactg ctgccaaagc gaaagtgaca
    39781 cggcggactc cactcgaaca aaagtcgata atgactaagg ctgaaacatt catgattgtt
    39841 gacgtacgtc gaaaagaccc tgcttgaggg tgacgcacga accgctgtct gcggttatat
    39901 gcccgatcaa gatcctgcaa aacgatcggg accgcggatc atagcctaaa ctgcgcaaga
    39961 gatcttctgt ttctcacaga ttttccctat ttatccacag gactttccag aactcgctaa
    40021 gtgtaaacga tcctgccgcg aggcgggcac gatttacgcc gcatattgga aaatttaatg
    40081 accatagaca aaaattggct taatcgatct aataaagatc caggacgatc cttgcgcttt
    40141 acccatcagc ccgtataatc ctccacccgg cgcgccatgc tggtttccac tggtgtgagg
    40201 tcgtacattt tccctgcgaa aaggtgcgga aaagcgcggt aaataaggaa agagaattga
    40261 ctccggtgtg tacaattatt acaatccggc ctctttaatc acccatggct tcggtgtcca
    40321 tcgtttcatt tttcggcgga tatccaataa agccattgaa tttattcaag tttaggtaga
    40381 aatcgccatg aaacgcactt ttcaaccgtc tgtactgaag cgcaaccgtt ctcacggctt
    40441 ccgtgctcgt atggctacta aaaatggtcg tcaggttctg gcacgtcgtc gtgctaaagg
    40501 ccgcgctcgt ctgaccgttt ctaagtaata aagctaaccc ctgagtggtt aagctcgcat
    40561 ttcccaggga gttacgcttg ttaactccca gtcaattcac attcgtcttc cagcagccac
    40621 aacgggctgg cacgccgcaa attaccattc tcggccgcct gaattcgctg gggcatcccc
    40681 gtatcggtct tacagtcgcc aagaaaaacg ttcgacgcgc ccatgaacgc aatcggatta
    40741 aacgcctgac gcgtgaaagc ttccgtctgc gccaacatga actcccggct atggatttcg
    40801 tggtggtggc gaaaaaaggg gttgccgacc tcgataaccg tgctctctcg gaagcgttgg
    40861 aaaaattatg gcgccgccac tgtcgcctgg ctcgcgggtc ctgatagccc tcattcgggt
    40921 ctatcaacgc ctgattagtc cgctactcgg gccgcattgt cgtttcactc caacctgttc
    40981 aagctacgga attgaggcat tgcgcaggtt tggagtgata aaaggcagtt ggttgacggt
    41041 gaaacgcgta ttaaaatgcc accctttaca ccctggtggt gacgatcccg tcccgcccgg
    41101 accatttgat accagagaac actaacgatg gattcgcaac gcaatctttt agtcatcgct
    41161 ttgctgttcg tgtctttcat gatctggcaa gcctgggagc aggataaaaa cccgcaacct
    41221 caggcccaac agaccacgca gacaacgacc accgcagcgg gtagcgccgc cgaccagggc
    41281 gtaccggcca gtggccaggg gaaactgatc tcggttaaga ccgacgtgct tgatctgacc
    41341 atcaacaccc gtggtggtga tgttgagcaa gctctgctgc ctgcttaccc gaaagagctg
    41401 aactctaccc agccgttcca gctgttggaa acttcaccgc agtttattta tcaggcacag
    41461 agcggtctga ccggtcgtga tggcccggat aacccggcta acggcccgcg tccgctgtat
    41521 aacgttgaaa aagacgctta tgtgctggct gaaggtcaaa acgaactgca ggtgccgatg
    41581 acgtataccg acgcggcagg caacacgttt accaaaacgt ttgtcctgaa acgtggtgat
    41641 tacgctgtca acgtcaacta caacgtgcag aacgctggcg agaaaccgct ggaaatctcc
    41701 tcgtttggtc agttgaagca atccatcact ctgccaccgc atctcgatac cggaagcagc
    41761 aacttcgcac tgcacacctt ccgtggcgcg gcgtactcca cgcctgacga gaagtatgag
    41821 aaatacaagt tcgataccat tgccgataac gaaaacctga acatctcttc gaaaggtggt
    41881 tgggtggcga tgctgcaaca gtatttcgcg acggcgtgga tcccgcataa cgacggtacc
    41941 aacaacttct ataccgctaa tctgggtaac ggcatcgtcg ctatcggcta taaatctcag
    42001 ccggtactgg ttcagcctgg tcagactggc gcgatgaaca gcaccctgtg ggttggcccg
    42061 gaaatccagg acaaaatggc agctgttgct ccgcacctgg atctgaccgt tgattacggt
    42121 tggttgtggt tcatctctca gccgctgttc aaactgctga aatggatcca tagctttgtg
    42181 ggtaactggg gcttctccat tatcatcatc acctttatcg ttcgtggcat catgtacccg
    42241 ctgaccaaag cgcagtacac ctccatggcg aagatgcgta tgctgcagcc gaagattcag
    42301 gcaatgcgtg agcgtctggg cgatgacaaa cagcgtatca gtcaggaaat gatggcgctg
    42361 tacaaagctg agaaggttaa cccgctgggc ggctgcttcc cgctgctgat ccagatgcca
    42421 atcttcctgg cgttgtacta catgctgatg ggttccgttg aactgcgtca ggcaccgttt
    42481 gcactgtgga tccacgacct gtcggcacag gacccgtact acatcctgcc gatcctgatg
    42541 ggcgtaacga tgttcttcat tcagaagatg tcgccgacca ctgtgaccga cccgatgcag
    42601 cagaagatca tgacctttat gccggtcatc ttcaccgtgt tcttcctgtg gttcccgtca
    42661 ggtctggtac tgtactatat cgtcagcaac ctggtaacca ttattcagca gcagctgatt
    42721 taccgtggtc tggaaaaacg tggcctgcat agccgcgaga agaaaaaatc ctgattcggt
    42781 gagttttcgc taaaataagg gcggtcagtt gaccgccttt tttcttttcg tagggcggat
    42841 aagcaccgcg catccgccac acaaagcaac aggaacatca tgagcgataa tgacactatc
    42901 gtagcccagg ccacgcctcc gggacgtggc ggcgttggca tcctgcgcat ctccggcttc
    42961 aaagcccgtg aagttgccga aaccgtgctg ggtaaactgc ctaagccgcg ctacgccgat
    43021 tatcttccgt ttaaagacgc cgacggcagc gtgctcgatc aggggattgc gctatggttc
    43081 cctggcccga actcgttcac cggcgaagat gtgctggaac tgcaaggtca tggcggtccg
    43141 gtgatcctcg acctgctgtt aaaacgcatt ctgaccattc ccggcctgcg gattgctcgc
    43201 cctggtgagt tttccgaacg cgcgtttctt aacgataaac ttgacttagc ccaggccgag
    43261 gcgattgccg atcttatcga cgccagttcg gaacaggcgg cccgttcggc acttaactcg
    43321 ctgcaaggcg cattctccgc acgggttaat catctggtag aagccctcac ccacttgcgc
    43381 atttacgtcg aagcggcaat tgatttccct gatgaagaga tcgatttcct ctccgacggc
    43441 aaaattgaag cccagcttaa tgacgtgatg gccgatctcg atgcagtgcg tgctgaagca
    43501 cgtcagggta gtttgttgcg cgaagggatg aaagtagtga ttgccggacg tcctaatgcc
    43561 ggtaaatcga gcctgttaaa cgcgctggcg ggccgtgaag cggcaatcgt aaccgatatc
    43621 gccggaacca cgcgtgacgt gctgcgtgag catatccaca ttgacggaat gccgctgcat
    43681 atcatcgaca ctgccgggct acgtgaggcc agtgacgaag tggaacgtat tggtatcgag
    43741 cgcgcgtggc aggaaattga acaggccgac cgcgtgctgt ttatggtcga tggcaccacg
    43801 acagacgccg ttgatccggc agagatctgg ccggaattta tcgcccgtct gcccgcgaaa
    43861 ctgccgatca ccgtggtacg caataaagcc gatatcaccg gcgaaacgct gggaatgagt
    43921 gaagtgaacg gtcacgcgtt aattcgtctc tcggcaagga ctggtgaagg cgtggacgtg
    43981 ctgcgtaacc atctcaaaca gagcatgggc tttgacacca atatggaagg cggcttcctg
    44041 gcgcgccgtc gccacttaca ggcgctggaa caggcagcgg agcatttgca gcaaggtaaa
    44101 gcgcaactgt tgggagcctg ggcaggtgaa ctgctggcgg aagagttgcg cctggcacag
    44161 cagaacttaa gcgaaatcac cggggaattt acttcagacg acctgctggg gcggattttc
    44221 tccagcttct gtattggtaa gtaaccgcgc ttacgaagcc gtattctgac tgtcagatgc
    44281 ggcttcgctt cattgttagc actcctgtta tgcctcaacc cttttttaaa cattaaaatt
    44341 cttacgtaat ttataatctt taaaaaaagc atttaatact gctccccgaa cgattgtgat
    44401 tcgattcaca tttaaacaat ttcagaatag acaaa
//
LOCUS       DAZPCS010000019        40891 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZPCS010000019 DAZPCS010000000
VERSION     DAZPCS010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40891
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.019"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(88..1338)
                     /gene="icd"
                     /locus_tag="ACHUQ7_000995"
     CDS             complement(88..1338)
                     /gene="icd"
                     /locus_tag="ACHUQ7_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFR1134513.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            1510..2163
                     /gene="rluE"
                     /locus_tag="ACHUQ7_000996"
     CDS             1510..2163
                     /gene="rluE"
                     /locus_tag="ACHUQ7_000996"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFR1134514.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILF
                     NKPYDVLPQFTDETGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            2173..2634
                     /gene="nudJ"
                     /locus_tag="ACHUQ7_000997"
     CDS             2173..2634
                     /gene="nudJ"
                     /locus_tag="ACHUQ7_000997"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFR1134515.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2688..3794
                     /gene="mnmA"
                     /locus_tag="ACHUQ7_000998"
                     /gene_synonym="trmU"
     CDS             2688..3794
                     /gene="mnmA"
                     /locus_tag="ACHUQ7_000998"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFR1134516.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3830..4471
                     /gene="hflD"
                     /locus_tag="ACHUQ7_000999"
     CDS             3830..4471
                     /gene="hflD"
                     /locus_tag="ACHUQ7_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFR1134517.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4475..5845
                     /gene="purB"
                     /locus_tag="ACHUQ7_001000"
     CDS             4475..5845
                     /gene="purB"
                     /locus_tag="ACHUQ7_001000"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFR1134518.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            6014..6685
                     /gene="phoP"
                     /locus_tag="ACHUQ7_001001"
     CDS             6014..6685
                     /gene="phoP"
                     /locus_tag="ACHUQ7_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFR1134519.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6685..8145
                     /gene="phoQ"
                     /locus_tag="ACHUQ7_001002"
     CDS             6685..8145
                     /gene="phoQ"
                     /locus_tag="ACHUQ7_001002"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFR1134520.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAQWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(8942..9223)
                     /locus_tag="ACHUQ7_001003"
     CDS             complement(8942..9223)
                     /locus_tag="ACHUQ7_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1134521.1"
                     /translation="MTEAEILRLIRRVSGISQQADEQTTQPDSVTAENYARVVAEVMR
                     RDGIQLNDADMRDIRIRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
     gene            complement(9237..10898)
                     /locus_tag="ACHUQ7_001004"
     CDS             complement(9237..10898)
                     /locus_tag="ACHUQ7_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFR1134522.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDNARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            complement(10882..11208)
                     /locus_tag="ACHUQ7_001005"
     CDS             complement(10882..11208)
                     /locus_tag="ACHUQ7_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFR1134523.1"
                     /translation="MDDIAVKQWREKSRQLAERGDLTPADWSNLELYCVNYSIYRKAV
                     ADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRRKNPPETEEED
                     ELDRLE"
     gene            complement(11264..11446)
                     /locus_tag="ACHUQ7_001006"
     CDS             complement(11264..11446)
                     /locus_tag="ACHUQ7_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1134524.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            complement(11430..11870)
                     /locus_tag="ACHUQ7_001007"
     CDS             complement(11430..11870)
                     /locus_tag="ACHUQ7_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFR1134525.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            complement(11870..12166)
                     /locus_tag="ACHUQ7_001008"
     CDS             complement(11870..12166)
                     /locus_tag="ACHUQ7_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFR1134526.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLAFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGGY"
     gene            complement(12163..12501)
                     /locus_tag="ACHUQ7_001009"
     CDS             complement(12163..12501)
                     /locus_tag="ACHUQ7_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFR1134527.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            complement(12498..13709)
                     /locus_tag="ACHUQ7_001010"
     CDS             complement(12498..13709)
                     /locus_tag="ACHUQ7_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFR1134528.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISDAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRYCLLSGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGDHNWRYCITDAYTGNIRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSDVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            complement(13711..14283)
                     /locus_tag="ACHUQ7_001011"
     CDS             complement(13711..14283)
                     /locus_tag="ACHUQ7_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFR1134529.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWADLAGV"
     gene            complement(14323..15480)
                     /locus_tag="ACHUQ7_001012"
                     /pseudo
     CDS             complement(14323..15480)
                     /locus_tag="ACHUQ7_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
     gene            complement(15781..16005)
                     /locus_tag="ACHUQ7_001013"
     CDS             complement(15781..16005)
                     /locus_tag="ACHUQ7_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1134530.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMNITDYRAFLETGALIEKDHHG
                     FVRCALSGEMLAVNPEQTDALIEFLKGIRD"
     gene            complement(16046..16159)
                     /locus_tag="ACHUQ7_001014"
     CDS             complement(16046..16159)
                     /locus_tag="ACHUQ7_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFR1134531.1"
                     /translation="MSRVGISEPHHAAGAIRLFLRHLKRYGALLAFRHGPA"
     gene            complement(16234..16308)
                     /locus_tag="ACHUQ7_001015"
                     /pseudo
     CDS             complement(16234..16308)
                     /locus_tag="ACHUQ7_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16318..16623)
                     /locus_tag="ACHUQ7_001016"
     CDS             complement(16318..16623)
                     /locus_tag="ACHUQ7_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1134532.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWE
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            complement(16620..16898)
                     /locus_tag="ACHUQ7_001017"
     CDS             complement(16620..16898)
                     /locus_tag="ACHUQ7_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1134533.1"
                     /translation="MHTSGKFNKSFKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            complement(17178..18926)
                     /locus_tag="ACHUQ7_001018"
     CDS             complement(17178..18926)
                     /locus_tag="ACHUQ7_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HFR1134534.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHQESEIVHVWRGGVWCPVSTMELSREMVVIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNALNFH
                     KWLEHAAGKDQRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            complement(18923..19222)
                     /locus_tag="ACHUQ7_001019"
     CDS             complement(18923..19222)
                     /locus_tag="ACHUQ7_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFR1134535.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFTLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            complement(19228..19461)
                     /locus_tag="ACHUQ7_001020"
     CDS             complement(19228..19461)
                     /locus_tag="ACHUQ7_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1134536.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            complement(19463..19683)
                     /locus_tag="ACHUQ7_001021"
                     /pseudo
     CDS             complement(19463..19683)
                     /locus_tag="ACHUQ7_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19676..19897)
                     /locus_tag="ACHUQ7_001022"
     CDS             complement(19676..19897)
                     /locus_tag="ACHUQ7_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1134537.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            complement(19887..20087)
                     /locus_tag="ACHUQ7_001023"
     CDS             complement(19887..20087)
                     /locus_tag="ACHUQ7_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFR1134538.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            20217..20396
                     /locus_tag="ACHUQ7_001024"
     CDS             20217..20396
                     /locus_tag="ACHUQ7_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1134539.1"
                     /translation="MSTHKNERRGNPPFQFRLDPDLREKMEKAQQQDGDDSLAAWIKR
                     IIRKELQQRGIEPKG"
     gene            20521..20850
                     /locus_tag="ACHUQ7_001025"
     CDS             20521..20850
                     /locus_tag="ACHUQ7_001025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1134540.1"
                     /translation="MNQNDSRRVLLPLRSTLKNTHQRRELRSYGNALNQTDCIAHQPD
                     SGYHPRAYVWDHTHKRRSGLSVQKGGSISGPLFLCLKNPNFVVFQFHQGERIPAHVLA
                     YIQSSWL"
     gene            complement(20900..21088)
                     /locus_tag="ACHUQ7_001026"
     CDS             complement(20900..21088)
                     /locus_tag="ACHUQ7_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR1134541.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(21463..22692)
                     /locus_tag="ACHUQ7_001027"
     CDS             complement(21463..22692)
                     /locus_tag="ACHUQ7_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1134542.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGEKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQETSVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            22941..24062
                     /gene="roxA"
                     /locus_tag="ACHUQ7_001028"
                     /gene_synonym="ycfD"
     CDS             22941..24062
                     /gene="roxA"
                     /locus_tag="ACHUQ7_001028"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFR1134543.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(24111..25337)
                     /gene="pepT"
                     /locus_tag="ACHUQ7_001029"
     CDS             complement(24111..25337)
                     /gene="pepT"
                     /locus_tag="ACHUQ7_001029"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFR1134544.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVILEGMEK
                     AVQVIVRIAELTAQRK"
     gene            25587..26723
                     /gene="potA"
                     /locus_tag="ACHUQ7_001030"
     CDS             25587..26723
                     /gene="potA"
                     /locus_tag="ACHUQ7_001030"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFR1134545.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            26707..27564
                     /gene="potB"
                     /locus_tag="ACHUQ7_001031"
     CDS             26707..27564
                     /gene="potB"
                     /locus_tag="ACHUQ7_001031"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFR1134546.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            27561..28355
                     /gene="potC"
                     /locus_tag="ACHUQ7_001032"
     CDS             27561..28355
                     /gene="potC"
                     /locus_tag="ACHUQ7_001032"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFR1134547.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            28352..29398
                     /gene="potD"
                     /locus_tag="ACHUQ7_001033"
     CDS             28352..29398
                     /gene="potD"
                     /locus_tag="ACHUQ7_001033"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFR1134548.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(29554..30375)
                     /gene="cobB"
                     /locus_tag="ACHUQ7_001034"
     CDS             complement(29554..30375)
                     /gene="cobB"
                     /locus_tag="ACHUQ7_001034"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFR1134549.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLEGL"
     gene            complement(30391..31302)
                     /gene="nagK"
                     /locus_tag="ACHUQ7_001035"
     CDS             complement(30391..31302)
                     /gene="nagK"
                     /locus_tag="ACHUQ7_001035"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFR1134550.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLVFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(31331..32575)
                     /gene="lolE"
                     /locus_tag="ACHUQ7_001036"
     CDS             complement(31331..32575)
                     /gene="lolE"
                     /locus_tag="ACHUQ7_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFR1134551.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(32575..33276)
                     /gene="lolD"
                     /locus_tag="ACHUQ7_001037"
     CDS             complement(32575..33276)
                     /gene="lolD"
                     /locus_tag="ACHUQ7_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFR1134552.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLLNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSCALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(33269..34468)
                     /gene="lolC"
                     /locus_tag="ACHUQ7_001038"
     CDS             complement(33269..34468)
                     /gene="lolC"
                     /locus_tag="ACHUQ7_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFR1134553.1"
                     /translation="MYQPVALFIGLRYMRGRAVDRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLRVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            34730..35803
                     /locus_tag="ACHUQ7_001039"
     CDS             34730..35803
                     /locus_tag="ACHUQ7_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFR1134554.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            35931..39377
                     /gene="mfd"
                     /locus_tag="ACHUQ7_001040"
     CDS             35931..39377
                     /gene="mfd"
                     /locus_tag="ACHUQ7_001040"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFR1134555.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGILIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKARNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            39520..40482
                     /gene="ldtC"
                     /locus_tag="ACHUQ7_001041"
     CDS             39520..40482
                     /gene="ldtC"
                     /locus_tag="ACHUQ7_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HFR1134556.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPASSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(40565..40777)
                     /locus_tag="ACHUQ7_001042"
     CDS             complement(40565..40777)
                     /locus_tag="ACHUQ7_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015705050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HFR1134557.1"
                     /translation="MSFANFAAVEVQSTPEGQQKVGTISANAGTNLGSLEEQLAQKAD
                     EMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT         9622 a        10793 c        10103 g        10373 t
ORIGIN      
        1 tatgctcgat atgctattaa taatatccac ataaataaaa caacgggcgt gttatacgcc
       61 cgtttaaata tttaacacat gtagtgatta catattcttg atgatcgcat caccaaactc
      121 tgaacatttc agcagtttag cgccttccat cagacgctcg aagtcatagg ttacggtctt
      181 ggcgttgatc gcgccttcca tacctttaac aatcaggtct gcggcttcag tccagcccat
      241 atggcgtaac atcatctcag cggagagaat aatagagcca gggtttactt tgtcctgacc
      301 ggcgtatttc ggcgcagtac cgtgggtggc ttcaaacagg gcgcattcgt caccgatgtt
      361 tgctccaggg gcgataccga taccgccaac ctgcgctgcc agagcgtcag aaatgtagtc
      421 accgttcagg ttcatacagg cgataacatc atattcagcc ggacgcagca ggatctgttg
      481 caggaatgca tcagcaatca cgtctttaat gacgatctct ttgccggtat tcgggttttt
      541 aactttcagc cacgggccgc cgtcgatcag ttcaccgcca aattcttcac gcgccagctg
      601 gtagccccag tctttaaacg cgccttcggt gaacttcatg atgttgcctt tgtgcaccag
      661 ggtcacagag tcacgatcgt tagcaattgc gtattcgatc gctgcacgaa ccagacgttt
      721 ggtgccttct tcagaacacg gcttaatacc gataccgcaa tgttccggga agcgaatttt
      781 cttcacgccc atctcttcac gcaggaattt aatcactttc tcggcgtcgg cagagtcagc
      841 tttccattcg atacccgcat aaatgtcttc cgagttttca cggaagataa ccatatcggt
      901 cagttcaggg tgtttaaccg ggcttggagt gccctgatag taacgtaccg gacgcaggca
      961 gatgtagaga tccagttcct gacgcagggc aacgttgaga gaacgaatac cgccaccaac
     1021 cggagtggtc agcgggcctt taatggcaac gcgatattca cgaatcagat caagggtttc
     1081 agcaggcagc cagacatcct gaccataaac ctgtgtggat ttttcaccgg tgtaaatttc
     1141 catccaggag attttacgct cgcctttata ggctttctcg actgcagcgt cgaccacttt
     1201 cagcatggct ggggttacat ctacaccgat tccatcacct tcaatgtaag ggataatcgg
     1261 attttcagga acgttgagtt tgccgttttg cagggtgatc ttcttgcctt gtgccggaac
     1321 aactacttta ctttccattc acctctcctt cgagcgctac tggtttgctc gtctgccacc
     1381 acgttgcata tgcgtttgcg tcctgcgata cggatgcttt agagcaattt tttgttaatg
     1441 atttgtaatt gacttgtcaa tactacccta ttgtttgccg tcatgaaaga tgcgcgttat
     1501 taggctataa tgcggcaatt cataatctct gaaaatacca tgcaaaaaac ttcttttaga
     1561 aatcaccagg ttaagcgatt cagctcgcaa cgttctacca ggcgtaaaca tgaaaaccag
     1621 cccacgcgtg tgatcctctt caataaaccc tacgatgttc ttccgcagtt caccgatgaa
     1681 accggacgca aaacattaaa agaattcatc ccggttcagg gcgtttatgc agcaggtcgc
     1741 cttgaccgcg atagcgaagg gttgctggtg ctgaccaata acggcgcgtt gcaggcgcgt
     1801 ttaacccagc cgggtaaacg caccggaaaa atctattatg tgcaggtgga aggtattccc
     1861 acacaagacg cacttgaagc cttgcgcaat ggcgtaacct taaatgatgg ccctaccctg
     1921 cccgcaggcg cggagctggt tgacgaaccc gcgtggttat ggccgcggaa tccaccaatt
     1981 cgtgaacgca aaagtattcc caccagctgg ctgaagatca ccttatatga aggacgtaat
     2041 cgccaggtgc gccgcatgac cgcccatgtt ggtttcccca cgctgcgact gattcgctat
     2101 gcgatgggag attactcttt ggataatctt gccaatggcg aatggcgaga agtgacagat
     2161 taaggatgca aaatgtttaa accgcacgtt accgttgctt gcgtggtgca cgcagaaggc
     2221 aaatttttag tcgttgaaga gacgattaat ggtaaagcgt tatggaacca acctgccggg
     2281 catctggaag ccgatgaaac cttagtggaa gccgccgccc gtgaactgtg ggaagaaacc
     2341 ggcatcagcg cgcagccgca acactttatt cgtatgcatc agtggattgc gccagataaa
     2401 acgccgtttt tgcgtttcct ctttgccatt gagcttgagc aaatatgccc gacccagcct
     2461 catgacagcg atatcgactg ctgccgttgg gtcagcgccg aagaaatttt acaggcgtca
     2521 aatcttcgtt cgccgctggt ggcggaaagt attcgttgtt atcaaagcgg gcaacgttat
     2581 ccgctggaga tgattggcga ttttaactgg ccttttacaa agggtgtcat ctaaaaggct
     2641 gcgtgataga atacgccgcc ttgaagttca atgtcgtgag tgatccaatg tctgaaaccg
     2701 caaaaaaagt aatcgtcggc atgtccggcg gtgtcgattc ctccgtttct gcctggctgt
     2761 tgcaacaaca gggatatcag gtcgaaggcc tgtttatgaa gaactgggaa gaagacgacg
     2821 gtgaggaata ttgcacagca gcagcggatc tggctgatgc ccaggcggtc tgcgacaagc
     2881 tcggcattga actgcacacc gttaactttg ctgccgagta ctgggacaac gtcttcgaac
     2941 tgttccttgc cgaatataaa gccggtcgca cgccgaatcc ggatattctg tgcaacaagg
     3001 agatcaaatt taaagccttc ctcgaatttg ccgccgaaga tttaggtgcc gattatatcg
     3061 ctaccggtca ttacgtacgt cgggccgatg tcgatggcaa gagccgcctg ctgcgtggtc
     3121 tggacagcaa taaagaccag agctacttcc tttatacgct cagccatgag cagattgcgc
     3181 aaagcctgtt cccggtaggc gaactggaaa aaccgcaggt gcgtaagatt gctgaagatc
     3241 ttggtctggt caccgcgaag aaaaaagact ctaccggcat ttgcttcatt ggcgaacgta
     3301 aattccgcga gttcctgggc cgttatctcc cggcgcaacc gggcaaaatc attaccgtcg
     3361 atggcgatga aattggtgag caccaggggc tgatgtatca cactctcggt cagcgtaaag
     3421 gtctgggtat cggtggcacc aaagaaggta ccgaagaacc gtggtatgtg gtggacaaag
     3481 acgtcgaaaa caacattctg gttgtcgctc agggccatga acacccgcgg ctgatgtctg
     3541 tcgggttgat tgcccagcag ctgcactggg tcgatcgcga accattcacc ggcactatgc
     3601 gttgcacggt aaaaacccgc tatcgccaga ccgacattcc gtgcaccgtc aaggcgctgg
     3661 acgacgatcg cattaaagtg attttcgatg agccggttgc cgccgtgacg ccgggccagt
     3721 ctgccgtctt ctataacggt gaagtgtgcc tcggtggcgg tattattgag cagcgtctgc
     3781 cgctgccggt ctgattatta tctttactta aaacaaggaa gcagtgaacg tggcaaagaa
     3841 ttactatgac atcaccctcg ccctggccgg tatttgtcag tcggcacgcc tggtgcaaca
     3901 actcgctcac caggggcatt gtgatgccga tgcgctacac gtctcactca acagtattat
     3961 tgatatgaac cccagctcga cgctggcggt ttttggcggt agcgaagcca acctgcgcgt
     4021 cgggctggaa accctgctcg gcgtgctcaa tgccagcagt tgccagggct taaacgccga
     4081 attaacccgc tacacactca gcttgatggt gcttgagcgc aaactctcct cagcgaaagg
     4141 cgcgctcgac actctgggca accggatcaa cggcctgcaa cgccagctcg aacacttcga
     4201 tttacagtcc gaaacgctga tgagcgcgat ggctgctatc tatgttgatg tgatcagccc
     4261 gcttggcccg cgcattcagg tcaccggttc ccctgctgta ctgcaaagcc cacaagtgca
     4321 ggcgaaagtt cgcgcaaccc tgctggcagg cattcgcgcc gccgtgctct ggcaccaggt
     4381 cggcggcgga cgtctacaac tgatgttttc tcgtaatcgc ctgaccactc aggcaaaaca
     4441 aattcttgct catttaaccc cggagttgtg atctatggaa ttatcctcac tgaccgccgt
     4501 ttcccctgtc gatggacgct acggcgataa agtcagcgcg ctgcgcggga ttttcagcga
     4561 atatggtttg ctgaaattcc gtgtacaagt tgaagtacgt tggctgcaaa aactggccgc
     4621 gcacgcagcg atcaaggaag ttcctgcttt tgctgccgac gcaatcggtt accttgatgc
     4681 aattgtcgcc aatttcagcg aagaagatgc cgcacgcatc aaaaccatcg agcgtaccac
     4741 taaccacgac gttaaagcgg ttgagtattt cctgaaagaa aaagtggcgg agatcccgga
     4801 actgcacgcg gtttctgaat tcatccactt tgcctgtact tcggaagata tcaataacct
     4861 ctcccacgca ttaatgctga aaaccgcgcg tgatgaagtg atcctgccgt actggcgtca
     4921 actgattgat ggcattaaag atctcgccgt tcagtaccgc gatatcccgc tgctgtctcg
     4981 tacccacggt cagccagcca cgccgtcaac catcggtaaa gagatggcaa acgtcgccta
     5041 ccgtatggag cgccagcacc gccagcttaa ccaggtggag atcctcggca aaatcaacgg
     5101 cgcggtcggt aactataacg cccacatcgc cgcttacccg gaagttgact ggcatcagtt
     5161 cagcgaagag ttcgtcacct cgctgggtat tcaatggaac ccgtacacta cccagattga
     5221 accgcacgac tacattgccg aactgtttga ttgcgttgcg cgcttcaaca ccattctgat
     5281 cgactttgac cgtgacgtct ggggttatat cgcccttaac cacttcaaac agaaaaccat
     5341 tgctggtgag attggttctt ccaccatgcc gcataaagtt aacccgatcg acttcgaaaa
     5401 ctccgaaggg aatctgggcc tttccaacgc ggtattgcag catctggcaa gcaaactgcc
     5461 ggtttcccgc tggcagcgtg acctgaccga ctccaccgtg ctgcgtaacc tcggcgtggg
     5521 tatcggttat gcgctgattg cgtatcaatc caccctgaaa ggcgtgagca aactggaagt
     5581 gaaccgtgac catctgctgg atgagctgga tcacaactgg gaagtgctgg cagaaccaat
     5641 ccagacagtt atgcgtcgct atggcatcga aaaaccgtac gagaagctga aagagcttac
     5701 tcgcggtaag cgcgttgacg ccgaaggcat gaagcagttt atcgatggtc tggcgttgcc
     5761 agaagaagag aaagcccgcc tgaaagcgat gacgccggct aactatattg gtcgagctat
     5821 cacgatggtt gatgagctga aataagcctc aaatcagtgc cggatggcga tgctgtccgg
     5881 cctgcttatt aagattatcc actttttctt ttttcacttt acctcccctc cccgctggtt
     5941 tatttaatgt ttacccccat aaacacataa tcgcgttaca ctattttaat aattaagaca
     6001 gggagaaata aaaatgcgcg tactggttgt tgaagacaat gcgttgttac gtcaccacct
     6061 taaagttcag attcaggatg ctggtcatca ggtcgatgac gcagaagatg ccaaagaagc
     6121 cgattattat ctcaatgaac atttaccgga tattgcgatt gtcgatctcg gattgcctga
     6181 cgaggacggt ctgtcactga ttcgccgctg gcgtagcaac gatgtttcac tgccgattct
     6241 ggtattaacc gcccgtgaaa gctggcagga caaagtcgaa gtattaagtg ccggtgctga
     6301 tgattacgtg actaaaccgt ttcatattga agaggtgatg gcgcgaatgc aggcattaat
     6361 gcggcgtaat agcggtctgg cttcacaggt catttcgctc cccccgtttc aggttgatct
     6421 ctctcgccgt gaattatcta ttaatgacga agtgatcaaa ctgaccgcgt tcgaatacac
     6481 cattatggaa acgttgatac gcaataatgg caaagtggtc agcaaagatt cgttaatgct
     6541 acaactctat ccggatgcgg agctgcggga aagccatacc attgatgtac tgatgggacg
     6601 tctgcgcaaa aaaattcagg cacaatatcc gcaagaagtc attaccaccg ttcgcggcca
     6661 gggctacctg ttcgaattgc gctgatgaaa aaattactgc gtcttttttt cccgctctcg
     6721 ctgcgggtac gttttctgtt ggcaacggca gcggtagtac tggtgctttc gcttgcctac
     6781 ggaatggtcg cgctgatcgg ttatagcgtc agtttcgata aaactacgtt tcggctgtta
     6841 cgtggcgaga gcaatctgtt ctataccctt gcgcagtggg aaaacaataa gttgcatgtc
     6901 gagttacccg aaaatatcga caagcaaagc cccaccatga cgctaattta tgatgagaac
     6961 gggcagcttt tatgggcgca acgtgacgtg ccctggctga tgaaaatgat ccagcctgac
     7021 tggctgaaat cgaatggttt tcatgaaatt gaagcggatg ttaacgatac cagcctcttg
     7081 ctgagtggag atcattcgat acagcaacag ttgcaggaag tgcgggaaga tgatgacgac
     7141 gcggagatga cccactcggt ggcggtaaac gtctacccgg caacatcgcg gatgccaaaa
     7201 ttaaccattg tggtggtgga taccattccg gtggagctaa aaagttccta tatggtctgg
     7261 agctggttta tctatgtgct ctcagccaat ctgctgttag tgatcccgct gctgtgggtc
     7321 gccgcctggt ggagtttacg ccccatcgaa gccctggcaa aagaagtccg cgaactggaa
     7381 gaacataacc gcgaattgct caatccagcc acaacgcgag aactgaccag tctggtacga
     7441 aacctgaacc gattgttaaa aagtgaacgc gaacgttacg acaaataccg tacgacgctc
     7501 accgacctga cccatagtct gaaaacgcca ctggcggtgc tgcaaagtac gctgcgttct
     7561 ctgcgtagtg aaaagatgag cgtcagtgat gctgagccgg taatgctgga gcaaatcagc
     7621 cgcatttcac agcaaattgg ctactacctg catcgtgcca gtatgcgcgg cgggacattg
     7681 ctcagccgcg agctgcatcc ggtcgcccca ctgctggaca atctcacctc agcgctgaac
     7741 aaagtgtatc aacgcaaagg ggtcaatatc tctctcgata tttcgccaga gatcagcttt
     7801 gtcggtgagc agaacgattt tgtcgaggtg atgggcaacg tgctggataa tgcctgtaaa
     7861 tattgcctcg agtttgtcga aatttctgca aggcaaaccg acgagcatct ctatattgtg
     7921 gtcgaggatg atggccccgg tattccatta agcaagcgag aggtcatttt cgaccgtggt
     7981 caacgggttg atactttacg ccctgggcaa ggtgtagggc tggcggtagc ccgcgaaatc
     8041 accgagcaat atgagggtaa aatcgtcgcc ggagagagca tgctgggcgg tgcgcggatg
     8101 gaggtgattt ttggtcgcca gcattctgcg ccgaaagatg aataaatatg tccttttacc
     8161 actacatcaa gtcattgatt cgatcaaaca attttcattg aaaaagtgcc aaaaatcgca
     8221 aattgactac accattaact acaccgttcg gtgcactgta tgaaacaacg tggaacaaat
     8281 agacacaaga aatatacagg cgggtaatct ttccaggggg aagcgcacca attcatgagg
     8341 ggcgttaatg tcgatatggg gatccccata acggggctac cggatttttt ttccggttaa
     8401 cctttaatca ggctggtggg ttttacatac ccttgatcac cgtaatgatg atccagatgt
     8461 gcaccttttc caccaatggc ggaaaagctc caaccgtaac gatgatcgct atggttgcct
     8521 cgatggtctg cgtggtcatt atgaccgttc agccagtgac cttaatcaga atttttcgct
     8581 aaaaaatgac gttggccacg tcatccggaa agcacaaaat cacgtcgatt tttaatgaac
     8641 ggttaaaccg tctttttgtc acggtgtgtc actggttgtc actctccgtc acgattgagg
     8701 tggtggtcat tctgcccgac cgcgcatctt tgcgctatcg ggaatatcaa acagttacct
     8761 cgccgacttc ctcaaattga ggtttccgaa aatatcagag cgggttagcg attaagctga
     8821 cgagtaatta aactgcgaaa atttcgtagt ttctgaatat attccggcct gctggttaac
     8881 agtcctcaga tgtgagggat gtagccagcg caattttgcg ctttgcttta atctcagtga
     8941 gttaccgccg taaccgctcc ggcttcttcc agtggtacgt gattttctcc ttctcccgat
     9001 acagttcaac gcggcgattg taggccagca tttccagaac gcggatccgt atgtcgcgca
     9061 tatccgcatc attaagctgg ataccatcac ggcgcatcac ctcagcaaca acacgcgcat
     9121 aattttcggc tgtcacgctg tccggctgcg tggtctgttc gtcagcctgc tggctgattc
     9181 cagagacgcg gcggattaat cgcagtattt cggcttctgt catgctgctg acctcattac
     9241 accccgctaa attcttccag tttctggcgg tggctgtcgc ttatatcaaa agcaaaatcc
     9301 tcatgctctg cctggaatgt accaaacgcc atcagcgccg ctacgctcgg gtctatcttg
     9361 ttcggtgatt ttttcttgtt cggcttgata ttggcgttcg cgtcactctg catcacgacg
     9421 ttactcatcg accaggacaa caccggatcg ccacgatgca caatcaccct gcggttaaca
     9481 aaaacttcga acgatttcgc cgccggactg aatctgaggt aggtttgcgg gaacggctcc
     9541 acctcaaaac cagccccctg taattgcgtc ctgagatgcg tggcgttcca cgtatcaaag
     9601 cccaccagcc tgatattaaa tttctccgca tcctgcatga tgtcatctct gatccggtcg
     9661 taatcaatgc agtcacctgg cgttgtgcgt atccagcccg ctttagccca ctggcgatag
     9721 acagcgcggt ttttattggc ggggttctgt agctggaact ccggcagata atgacgggaa
     9781 atcagcataa tggttttacc gaccggaaag gcatagcata cgctggaaat atcgctggtt
     9841 gatgataagt ccagccccgc gtaacactcc tgaccgtgta aatcttcctc cgtgaacgtt
     9901 ccggcacact cagcccatgc accattaccc atccacgggg tagtcccctg acaccagata
     9961 ttaaatcgct ttgtcatcat ctccacccac tgcgacggaa taccccgcgc tttctggatg
    10021 gttgaggcca gtttttcacg atccacggaa acatcaatat tagggttcgc ctttatccac
    10081 atcgccggat catcaacctc gttttcgtcg tccagttcgt aaatcagtac aaaaattgaa
    10141 tcgttaacct cttcgccgtc caggatctgg cagcaataat catagtgctg tttacaggct
    10201 gaaacgacgt tactgcccga tgtggtaatg gcaaataaca gcccttcggg acgtgcgccc
    10261 atccccagct caagcgcgga ataaacgccg ttatcggggt gtaggtggta ttcgtccacg
    10321 atggaaagac tggggtttgt cccctcgatg gttgccgctt ttgctgccag cggctttaac
    10381 aggctgttgc ttttcgggtg tatcacctta tgcgcctgaa tattcacccg cctgcgtaac
    10441 ggtcgggata aaaggcacat ctgacgcgca ttatcaaaca cgatccgcgc ctggtcacgg
    10501 ctcaccgctg cggtgtagat atcctgctga ccgttctcca taatcagaaa ccagttagcc
    10561 agaatcgcgg cggtcgtgga tttcgcattt ttgcgcggca cttcgataaa ggcgctcgtg
    10621 tatttgcgcc gtccggtggc cttaacctta aagccgagga tgcacgcaaa ggcgaactgc
    10681 tgccacggct ccagctcaat gggtctgcca cgcatcggtc cttttacgtg cgggcacacc
    10741 ctggaaaagg caataaaccg ctccacaacc tcacgatcga acgtgtaaag ggggctttta
    10801 aggtccgaaa agtaccgttt aacagcctgt tttaaccgtt tacaggccgg aattttgccc
    10861 gtttttacgt cttctgcgta cttattccag gcggtcaagc tcgtcctctt cttctgtttc
    10921 cggtggattt ttacggcggc ttatcgggtc aaaaccgagc aaggaggcca tttttatcat
    10981 cactctttca gcgtcggatt ttgcgcttaa tgcggggttt ctgctctcgc cgccctgact
    11041 gttaacaatg ctgaacccgc gcgccgcaag gtctgcaacg gctttccggt aaatggagta
    11101 gttgacgcaa tacagttcca gattgctcca gtcggcgggg gtcaggtctc cccgttccgc
    11161 aagctgccgc gatttttccc gccactgctt cacggcgata tcatccaggt aggcggggtc
    11221 gggtttgtcc tgaaaacgcc ccccaccccg tcatacagcc tcatcagcga ttgcggaaac
    11281 attccataac ctcgcggtca cggtcggtta atcgcttcgc tgtgatgcgt tctgcgcgtc
    11341 ctgacgcttt atctttatgc cctgtttcct gtgtcttcca tgcgtcacgc tgccttataa
    11401 gtccacggat aaggcggttt tgttcccgct cattcatcag cgtcatacat ccagttattg
    11461 cggttagcag cccgttcttc ctcttcacga aatccacctg cggcacgctt gctttttgtt
    11521 gccggatcaa gccatttcgt tttctggtta tgacacgcct ggcacaatgg ttgatggttc
    11581 cattcgggcc agaagagaac atcatcaccg ccattaatcg ggataatgtg atccaccacc
    11641 acggcgggcg tatatatccc cttctcaagg catcgcacgc ataacgggtt tttactcaga
    11701 tacatggcgc ggtatttgtc ccactgtctg gaatatccac gcgcgcggcg gtgtcctcgt
    11761 ctggcatcct ctgcacgcca tgccgcccgc ctgtgctcct cacacttgcc ggatttaacg
    11821 cgcctgttac agcctggttc tgtgcatcgt cttaatggtt gccacggcat cagtaccccc
    11881 ccacatcacg gtaagccgtc cagagtgcgc ctatcgtcat gggtacgcgt gtttttgcgt
    11941 tatccgcgac aatctggcga ttttcataca ggtgagcgat aaacatcatg cagccaatct
    12001 ttatggctgg cgtgaacgcc agcccgtcat caaagcgcct gcctatgtgt atctggcacg
    12061 cctcaagcga tgcggcaatg tatccgctga ttaactggtc ttcatcgtcg ccatcgatgc
    12121 ggcaatggag tttcacttct tccagggtga taagttcttc tgtcattttt ccgcgccctc
    12181 acgacaaaga atttcaaggc gtgtcctggc ggcatccggc agcggctgcc cgatgatatt
    12241 cagtacacgc cccgccagcg gccccgtact gacctttatc cggctggtgg cgttgatgtc
    12301 cttccggtac cgtatccaga tccttacggt accaatcgcc agctctgcgc ccgatgaaat
    12361 ggcctccttg ctgctgacca tgttcacgct tgcccagagt gtgtgaccgt cctcccacgt
    12421 ttcgagtatt tcgccggtta tatccctggt ctgtttcagg gtctgaatcg ttatcctgtc
    12481 acgcagtcgc cctatgttca ttccggtttt tctccctcgc tgatttttac ttcctgtttc
    12541 catgcctggc tgaactcatc accaccatca cgcggtgaca gcccttcacg ttcgcgggct
    12601 tcgttcggac acataacgtc ggattttatg ccgcgctcat aggtggcaaa gcgttcgcca
    12661 ggtgtggccc gtaacaggtc cgcgctgtca aactccacct gataccggat accaggtaca
    12721 ggcgaggcca ccagcaaggc gttttttatc tgctgctcaa agttcgccag ccacgggcgc
    12781 atcgtcatgg tgagaaatgc gcggcttgcc tcgctgaagt tgctgtaggt gctgttgctg
    12841 tattcctgca aaaaaatcgg cgagacgttg aacattcggg cgatgtcttc aatggtgaag
    12901 cgacgggagg ccagccattc ggcatcctga ttactcatgc ccagctgctg atagctcatc
    12961 cccccttcaa ggatgggcgt ttttccggcg tttctggccc ctttgtagcg ttccagtgcg
    13021 gctaatgcct gtttgccctt cacgccgtcc agccattcgc ctgacgtgat aacccctgcc
    13081 gccatcatgc catcacgcat cacgctcgcg ccgtggcgtt gttgggccag cccaagcccc
    13141 agcgattcgc ggcagatggt tacaggtgag cgccccataa aaccgtcatc cgtggagtaa
    13201 cgaaggtgga gaacctccca cggtaaatag ttgcggatgt ttccggtgta ggcgtcagtg
    13261 atgcagtagc gccagttatg atcacctgtc tgctccacgt tcaccgactg cggcgggtaa
    13321 ggatgtaaag ccgccggaaa accatcacgc ccccactgaa taaccgcata agcattacca
    13381 cttaacaggc agtagcggat catcattcgc ttaaactggt agggtgtctg ccacgcgttc
    13441 gggcgctcgt tgaggatgtg atcgaccgga tgagaatcaa gccattcgcg ggcctccttc
    13501 cccttctcat tgcgtaccag gtacaggtaa cacggcatgg tagccaccgc gtcagagatg
    13561 accgtgacgg cgttcatgac ggcggggagt gattccgctg tcccagatga tacgtactca
    13621 cccgcccccg tgttcgatgt gccggccagc gccataaatt catcaagcgt catgctgcgc
    13681 tgctcttttt ttcgtctgaa aggccacata tcacaccccc gctaaatccg cccaccagcg
    13741 gcgattatcc gcacgcggca ttttttcggg gtgctgctca tacagggaac ggcgggccag
    13801 ctccacgccg gaatcggggt aagccggtac cgatgtaacg gtaatttcgt acagttccgc
    13861 caccagcacg gtgcgcacgc atggatctgt tgtggtatcc catacatcct tacgggagcg
    13921 aaagccaaag ctcatccccg agatgtcccc acgcttaacc agttcgataa cgtcgcgccc
    13981 tgtactggta tccggtgggg tcagttcaaa gcgtaaccct gtttcgtcct cttccagttt
    14041 cagcgtgccg gaacgggtgc gccccagtaa catgctgtgg tcatgctcat acaggccgcg
    14101 aacgtcatta cccgccgcaa gccattcagt aaacgccccc cgctggaatt tttcgtaaaa
    14161 ctcaccccat aacagttctg aaagcttatc ccagcgaaca acgtagcccg tcagcgtacc
    14221 ggctccgctg gtggtgattt ccgatgaccg gatttccata ctcttcataa ttttttcacc
    14281 cataaaacac taaaggggct ttttagcccc ttctgtatgc tgttaatcgt cgtcttccgg
    14341 cagtgccaga atcttgatcg cgttcgaatc caccacgcca ccgcctaaat atttttgcgt
    14401 gaaaattttg atgaatcctg gttctgtcag gttgtccggt ctggtgcgaa cacctgtttc
    14461 gtgatcaaca atgtagtaac cgcgtttgaa gtcacccagg gcaataacgt tatcaggcat
    14521 aaactccaga tattcgaccg gaaggcccag caacgtatca ggatcacccg cctgtaaacg
    14581 gtcgcgccag atataatcac cgttcgcgtt cttcaccttc tgaagttttg ccgccgtcgt
    14641 ggagttaacc actcagaccg cgtttttgcg gtatttttta cgtaatgcaa atttcaggtc
    14701 gatcagcggg tccgcagatg tccacgccag agattcggaa ggtttaatta cctgcaacgt
    14761 accaaaatca cgctctttgt cgttcttctc tgcacggggt acggataaaa aaccttttgc
    14821 ttttttgtca ccgtcgccca caaccagatc gctttcttcg gtttccgtga aggtgtcgcc
    14881 aatctcaccc gtcagccatg aaaggatgtc cacatcggaa aaatccacga tttcctgtgt
    14941 ggtgcgcggg tacgcataga ccggatacag cttaatgctc acctcgttaa tctgcggggt
    15001 gctggtctgt tcgcgtgcct taccctcttc accgtggtta acggtcgcac cgccagcgga
    15061 aacaagctgc ttaaactcgt tgctgctgat tttcttcacg gtacagatgc gacgcatggt
    15121 ggattcatcc gccagcattc gcatgatttc ggtgttcagt tcagggataa cggtataacc
    15181 accatcagcg ggaacgcctg tacttaatgc gcgggtttca ccggtcagaa tgtagttacg
    15241 tagttctgcg gggtcagtgg tctggctgct tttacctggc ttgctgcgct cttcgtctgc
    15301 aatggcttca aggcgggaaa tgtctttatc gagggattca gctttagcgc gtaattcgtc
    15361 aaattttgcg ccctcagcat cgttaagact gcggttttct ttttcagcgt tctccagcat
    15421 gtcgcgcatc tgatttttaa tggcggtttt ctgctggcgt aattcgatta ttctctgcat
    15481 aaaaaaagtc ctggggttaa gtaaggaact ccaggacgcg gcaaacactc aaccgttttt
    15541 cataaggaaa tcagtaatcg caccgatcgt tttcccgcct ggtaatgaat attggcgagc
    15601 acattaacag gcgggaaagt ggccccagcg tcctggcacc acgggcgaga ataatcatga
    15661 ttcagttcgg gtaaaatatg ccgatccgct cagtgaacaa cgtggaacaa ccacgaacaa
    15721 ataatttaca aaaaataaca aaaagccgga ttgctccggc tgtatgtgct gtatgggtgc
    15781 tcagtctctg atccctttca gaaattctat caatgcatct gtctgttcag gatttactgc
    15841 cagcatttca ccggatagcg cgcaacgaac aaaaccatga tgatcctttt caatcagcgc
    15901 ccccgtttcc agaaatgcgc ggtaatcagt gatattcatc gtctccatgt tgtcagcatg
    15961 gtatttatca cgctgtttaa gtatctcatc aaatttcatc ggcattgcgt tttcctctgt
    16021 tgtgtctgtt tgtttcagat agtaactatg ccggaccgtg acgaaaagcc agtagtgctc
    16081 cataccgttt caaatgacgc aaaaaaagcc ggattgctcc ggctgcgtgg tgtggttctg
    16141 atattcctac tctgctaatt tgctcagccc catgtctgcg taagtgcgat ttactgcatt
    16201 tctcaggtct gcgtagttct ccgttggcgg ctccggtgtg cccttgctgc tctgttttac
    16261 cagcgggtcg cccaccagct gcccgtaagc tgcaatttgt gctgtcatat tgccacctca
    16321 gtgaagcgat ccggcattgt tctttttcat ctcttccatc attttttcag tgagcattac
    16381 gcactgaaac cccatgtttt tgatgtctac gggtttcatg cctttgtcag tggatatcac
    16441 aggcagaata tcctgataca catcgcggtc agggtaacta agcacacaaa gctcccatga
    16501 tgccacgggt gaggatcctt cgtctgtttc aacccaccag cctggtgtag ctgcgcttat
    16561 ttgcagaatc gtgatccgtc cgttgtgctt gtaaaatttt tcaccaaatt ttggttcgct
    16621 catgattcca cctctccggt tttaacgttg attgttgtta cctgttccgc ttcggcaatc
    16681 tcccgctctg tcagcgtggc aaaatttgcc gccgtcgtgg tcatgaatgc gcttatcagt
    16741 tcgggatgtg ctttcgcgta tccttccccc gcgtggcggt ctatcgttct gattgccacc
    16801 tttaaagcgt gctctgtcat gtctaacgcg cgatatttcg gttctgttct gtctctgcgt
    16861 tttttgaatg atttattaaa tttccctgaa gtgtgcatat ttatttttac cccctcgttt
    16921 aaaaagtttt gagttgtgcc tccccttgtc taccttatct accttagtgg ccctcatgcc
    16981 agtaatggcg ctgctttcag cggggtagag tgcttttatc cactatctac cccgtgtcta
    17041 cctccctgtc tgattcaggt aaaatcaggt agagagggta gatagtgggt agacagtaaa
    17101 aaaaggctat ctacctaact taatgcactg aattaaatgt atttttcttt actcaggtag
    17161 acagcaatta caaaaaatta taaaaacgcg tcgcactcgt ctgttgttat tgcgttagtc
    17221 tgcgttactc ccttaacttt ccgcgtaata tattcatgtc cgtaaacttt cgcggctggc
    17281 ttcatagcct tgccaaagtc atttacgttt agcggtttgc tcctgcctgc gtacgccata
    17341 aacgccagat agacgcggta aaggctgttt ctggtcgtgt acttcactga atcgccaccg
    17401 ccgcccatca tcaggccgcg tgcttcctcc agaaaattca ggaactggca aaactcaata
    17461 accggatccg tctgttgctt tattgccaga gcttcatcac cgtcacgctg ttccagtagt
    17521 aaagcccgtg ctttctcaga gtcggtaaag ttcgccagca atcggcggat aatgacaggg
    17581 atttcagccg caatcttttc cggtagctcc ctgtcttttt cggcctcact aacgatattg
    17641 tcgaaacgga aaatcacgcg acgacgtgcc acacctccgg cccgttcggt gaatatcatc
    17701 gggttattgt tggtcgccag caccaccgcc ctgattacag ccgtgaaacg cttttcgtat
    17761 ttcgggttaa tttccacggg gtcaccgccc gtgattttct tgatgcccgt tccttcgcct
    17821 gtatatttcg gctggtcagc caggacgata agacgactcc cgacaacctg cgcacgtcca
    17881 ccagcatcat caagcgatgt catttcagcg cttaccgtgt tctgtttccc tgccagaagg
    17941 ctggctatgt gtgtgaatgt acttttaccg ctcccgccgt ctccggtggc ctcaataaac
    18001 atctgccagt cgtaccggtt cgccataatc atgtacagcg cggcacatat acgcatcatc
    18061 ttgcgctggt cttttccggc tgcgtgctca agccatttat gaaagtttag cgcgttatcg
    18121 cggatgttct cccctggtgc tggtggcgtg tactcaatgc cgttgtgcgt ggtgatccag
    18181 ttctccggcg tgtgcgggga aaattccccc gttttcaggt caagcgcacc attggcgaac
    18241 ggcagcaaat cgccggacgg ctcgcccatt ggttcggcaa taacttttaa cgcttccacg
    18301 gcgttattga ttacgcgctt gctgaaagtg gccctgtgct ctgaatagat caccaccatt
    18361 tcgcggctaa gttccattgt gctgaccgga caccataccc cgccgcgcca tacgtgaacg
    18421 atttcacttt cctgatgcac acaaacgcca tcaaagcgct cggcaagcag ctgcgcgcgc
    18481 tcactgtctg ccatttgtgc cagttgcgta tttttctgct ccggcatggt aagccctgcg
    18541 gcaatatttt cacgctcagc attcagatag cgccgccagt tttcacactt ctggacgtgc
    18601 attccttcgg ggtaaaaatt tgcgtcctga atatctgccg ccgccagctt ctgaccaatc
    18661 tttttggtct ccaccaaatc cagttctccg gcctggtaca gccttacgcg tttttttcca
    18721 tccggaacaa ttttcagtgc atcaagttcg gcaagctgat ttggcccaag ccacacaggc
    18781 ggcacattat cgccggatgc ggggccatcc tgctcctgcc attgtttcgc gtgcgaccag
    18841 gcatcactac ccgcaaagat aattacctct gtgtctttgt gttttattcc gcgtggctgt
    18901 ttttttacgt tcggtgccag tttcattttt tacccctgaa tacgttaagc atctttttta
    18961 tttcctgaat attggcgcgt gctttctccc tgctggttgg tttactgcgg ggcgctgcct
    19021 gtaccagcgt gaaatcacgc cggaattgat aaacaggcat cacgcaatca tattcgtaac
    19081 cttcacggcg gtaggtgatg cgccgttctg ccacgccttt aatcgttacc atgccgccgt
    19141 actggtcgcg gtaaatatca ccgcgcgtaa atttagggtg agtgttgcca ctggcagtta
    19201 agccagaata tttaagtttc attattttta ttctccggtg tgttgttctt tatatctgtc
    19261 gtgcaataga tctatttctt gcagttccat tattacaggc tcaagaagcg ttattaatgc
    19321 cgtggcaatt cttgattttt gtttgtcgcg ttcattgtcg ccaagtgttt caagccatat
    19381 gcgcaatatt tccagcatgt tttcactgtg agaaagtgca agaaatgcgc ggtctattgt
    19441 ttcgtggtaa atatcacgca tattaatccc cgtccgtcgt ttttcttaaa ataacctctg
    19501 tcacgaaatc agcataatcg gcggcgatat tcagtacatc aagcccgttg atttatattc
    19561 tctcgtggaa agtaagaaaa aagccgctct aataagttct ggcattgacg aaagcgcatc
    19621 agccgcatca tcaggaacgc cggaaaattc ctgtttcagg gaattaaaac gatcatcacg
    19681 cataaccccc cccattttca caatcggcaa caagaatatt tttagcgtca ttcagtgacc
    19741 gcgttgcgat gtatcggata cattccaggg cgaactgtgt ccggtctttc tgttcttctc
    19801 cctgcgcaag ctctgccgtg cattcaatat caataagcgc gtgcatcagc gtagtgagtg
    19861 cggcggctgc tgcgttcggt gtggttttat tgtgcatagg tttccccctg acgaatacgg
    19921 gcggcgaata cagcaacaca accggacggg caacggctac gcgcttcgcg ttccgtccaa
    19981 gcggttacgt ggatgatttg ggattctccg gcactcagtg ccagaaaacg ccacacaaag
    20041 gccgtttgtg tgtgtacaag gtgtggtata tgatttacgg caaccataac ggctcctagt
    20101 ttacgttgtt ggttagaagc cccgttactg ctcctacagt gcggggcttt gctttttgtg
    20161 ttgattgtgt gtaatgtgtc actacacata aatacattac ctgaggtgta attaacgtgt
    20221 caacacacaa aaacgaaagg cgtggtaatc ctccgttcca gttccgttta gatcctgact
    20281 tacgggaaaa gatggaaaag gcacaacaac aggacggcga tgattctctt gctgcctgga
    20341 taaagcgaat aatccgcaaa gaattacagc aacgcggcat agaacccaaa ggataaaaac
    20401 tatcagcgcc gtggtgtgag gtactacggc gcattgcttt acaggcgcac acaatgacca
    20461 acgaagaatc aaccgaaaaa acatcacctg cacgaaaaag acgacgcaga aagaaagagc
    20521 atgaatcaga acgattcgcg ccgtgttctt ttgcccttga gaagtacctt aaagaatact
    20581 catcaaagga gagaacttcg cagttatggc aacgcactga accagactga ttgcattgcc
    20641 caccagcctg atagcggcta tcatccccgt gcttatgttt gggatcacac acacaaacgg
    20701 cgcagcgggt tatctgttca gaaaggcggc tccatttcgg ggccgctttt tttatgcctg
    20761 aaaaacccca attttgtggt tttccagttt caccagggcg aacgaatccc cgcccacgtt
    20821 ctggcgtata ttcaatcttc atggttatag ctctgtgttc agatgattga tgtgtggcgg
    20881 ctgcgtgccg ccagcgtgat taatgaactg ccttgcagct atccttccag gccagaacct
    20941 cggataaaga ccagccaacg gaacgaccgc caagtttacg acgtgatggg aattgtccgg
    21001 ccttttccag gcggtagcgg catgagcggc taaggcctgt tagcttttcg cattcttttt
    21061 cacgtataaa ccgatcagtg cttaacacta ttgccccctt tcgtttctta aagagttatt
    21121 tcgtgttcta ttgcgttggg atgtgtctga ttgtgtcagg atgattcaga gttggcaaat
    21181 gttgaggccg tatggtttac agaaagagga ataatcagga taaaatcctt taaattcatg
    21241 ttaatacaaa ggcataaaat attgtttcat gccttttttc tcgctattta aagagtgatt
    21301 cgctagtgta taaaaaatca gtaacacatt aaaaatcagc tacttataaa tctgtacact
    21361 ttttcacctc ttgttcgtag ttgttccgca ttgttgctca ttgttgcacg ttgtatctgt
    21421 tcgcatatcc agtatgcgca tactgaaaaa acacgaaaaa aattattttc ttctggctac
    21481 tggtagcgtg gttacgtttt catgtgttcc cgccagtatc cctaaccgct ccgtccacat
    21541 atccagcgca ttgcgtttag catccagata acgggaatga ttataaactc gctgcattcc
    21601 tggcatctgg tggcctgtaa gctgctccac gacgtgagga tccacgccta aatcgttcag
    21661 catcgttgta aaggtgcgcc ggatgtcatg tagtgaccag tgagggtgat ttagcctcct
    21721 gtgcgctaat ctgccgtact gcgacacgct tgtttcctgt ttcacttccc ccagcaataa
    21781 gcccgtgtgc ctgttctgct ccaccagctg cgtgacgaac ggcagtattg cttccggtat
    21841 gggccggaat attgccacct tcgttttgct gtgttctttc ggcacggtcc agagcatttc
    21901 ggtaaaatcc cactcgctga tctccgataa cctcagttct accgtccggc atccgaacac
    21961 aatcaggagg cggattaacg cgatgtagta gggggagaat atttttttgt ccagtgcctg
    22021 caataattcg cccagttctt tggtgcttaa gacacgctcg cttatatccg gtttttcccc
    22081 aacgtccgcc acactcatat catcaagaac gttgctgatt gcatagcgcc gcctccggca
    22141 gaacttaagc gcctgtttgc acgtctgtag caagaatccg gcagtaacag gcgttcgctt
    22201 tgccacctgg tcaaaacagg ccagccagtg ccgtagctcg catttatcca gcggcatagc
    22261 accaatgtgc tgtattacgt gattattaag gcgctttttc agggcggcat aatccacgcg
    22321 gttttccttt gcgtacgact caagccagta ggtgagcgca tcgccaaccg ttaccggctt
    22381 taacgcttcc tgtacggtgt aattaagctc atgacgtgga tttttcccct ctgccagcca
    22441 tgcgcgacac tgggcggctt tttccctggc tgatttcagg ctcagatcag gataatttcc
    22501 cagcttaatg cgttccggtg gtgcccccct tcccgttccg gccctgtaag tgaaatacca
    22561 ggttaaaagg ccactggttg aatgcctgac gctcaggttt ccaccgtcat taagaaaggc
    22621 tgttttttgg gcgggtgtgc cgttgatttt cctcagctgt gtatcgctca gtttgttaag
    22681 tgctctgctc ataattttga ttccggtcat cacaattaac tacaccatta actacaccga
    22741 tcgttgcaca aagggctaca acgtgaaaca agctggaaca aggaaaatca caaatctgtt
    22801 tataatcaaa aacataatga acaatctgaa acattatgaa acggcaaaaa acactaattg
    22861 ataaaatatg tccatacttc acgcattacg ttaagcatcc gttataatcg gttgcagata
    22921 ccagcctgtg gatgcttaac atggaatacc aactcactct taactggccc gattttcttg
    22981 aacgtcactg gcagaaacgc ccggtggtgt taaaacgcgg ctttaataat tttattgacc
    23041 cgatctctcc agacgagttg gcgggtctgg cgatggaaag cgaagttgac agtcgactgg
    23101 tcagtcacca ggatggcaaa tggcaggtca gccacggccc gttcgaaagc tacgatcatc
    23161 tcggtgaaac caactggtca ttactggtac aggcagtaaa ccactggcat gagccgaccg
    23221 ccgcgctgat gcgaccgttc cgtgaactac cggactggcg tattgatgat ctgatgattt
    23281 ctttttctgt acctggcggc ggcgtcggcc cgcatctcga tcagtacgac gtgtttatca
    23341 ttcagggtac cggacgtcgt cgctggcgag tgggcgaaaa gctgcaaatg aaacagcact
    23401 gcccacatcc ggatctgtta caggtcgatc cgttcgaagc catcatcgat gaagagctgg
    23461 agcctggtga tattctttat attccgccag gattcccgca tgaaggctac gcgctggaaa
    23521 atgcgatgaa ctattccgtg ggttttcgcg cgccaaatac gcgggaatta attagcggat
    23581 ttgccgatta tgtgctgcaa cgtgaactgg gcggcaacta ctacagcgat ccggatgttc
    23641 cacctcgtgc tcatcctgcg gatgttctgc cgcaagagat ggataaactg cgtgagatga
    23701 tgctcgaatt gatcaaccag ccggaacact ttaagcaatg gtttggcgag tttatatccc
    23761 agtcacgtca tgaactggat atcgcgccgc cggaaccgcc ttatcagcca gatgaaatct
    23821 acgatgcgct gaaacaaggt gatgtgctgg tgcgcctggg tggtctgcgc gtattgcgca
    23881 ttggcgacga cgtgtatgcc aatggtgaga agatcgattc cccgcaccgt ccggcactgg
    23941 atgcactcgc cagcaacatt gcgctgactg cggagaattt tggcgatgcg ctggaagatc
    24001 cgtcattcct cgcgatgctc gcggcgctgg tcaatagcgg gtattggttc ttcgaagggt
    24061 aagtttggat ttaagccgga tgcagcatca catgccgcat cccttttcgc ttacttccgt
    24121 tgcgccgtta actcggcaat acggacgatc acctgcaccg ctttttccat accttccaga
    24181 atcacaaact catgcttacc atgatagttg taaccgccag tgaacaggtt cgggcaaggt
    24241 aatcccataa acgacaactg cgcgccgtcg gtaccgccgc ggatcggttt cagttccggt
    24301 tcaatatcgc agtcacgcat cgcctgctgg gcgatatcga gaatatgcgg atgctcaacc
    24361 actttctcgc gcatattgta gtaactgtct tcaatcacca attcaatgta gcaatcagga
    24421 tgtaaccctt tgcccacttt tttggcgatc tccatcattt tacgtttacg cgcttcaaac
    24481 tgtttacggt cgaaatcacg gatgatgtag tgcatatcgg cccgttcaac ggtgcctttc
    24541 atgctcgcca ggtgatagaa accttcatag ccttctgtca tttccgggct ttcatccgcc
    24601 ggaacttccg catgaatacg tgccgccagc gacagcgcat ttaccatcac tcctttcgcc
    24661 gtgcccggat gaacattgtt accgacaatt tttatattga ccgatgcggc gttgaagttt
    24721 tcaaactcca gttcgcctac gccaccaccg tcaacagtgt aagcccagcg ggcatcgaag
    24781 gcatcaacat caaaatgttt cgcccctttg cccacttctt catccggggt aaaggcgacg
    24841 cgaatatcac catgcggaat gtttttctgt tgcaataccg ccagcgcggt catgatttct
    24901 gcaatacctg ctttgtcatc agcacctaac aaggttttac catcggtggt aatcaacgtc
    24961 tgacccagta gctgatgcag caccgggaac ataaccggtg ataaaacttc atcgccgata
    25021 cccagcgcaa tatcgccacc gcgatagttt tcaacaattt gcggattaac atttttgccg
    25081 ctgcaatccg gtgaggtatc cacatgagaa ataaagccaa tcgccgggat atcgccaggg
    25141 acgttagccg gtaacgtcgc catcaaagtg cccttctcac ttaaggtcac attgataagc
    25201 cccatttctt cgagctgctc tttcagcaga tgcaataact tccattggcc ttccgtgctg
    25261 ggaacctgtc tcacccctgc ttttgattgg gtatccagag acacgtagtt caaaaatcgc
    25321 tcaagtagtt tatccatgta gtcaccctca ctttttgtga caacattatt aagaagcaag
    25381 aaaagacaaa tattgcgtca ggtcactttt acccctgcaa gcgggaatat ttatcagcat
    25441 taccttaatg aatataaagc taagccagta attcacaaca aagattggcg attcaagcgg
    25501 tttgccgtag aattaccgcc ctcattagga gtcaccaagg tggttaacca caaaccccgc
    25561 atcggtaagc catccgttgc gtttacatgg gacagagtaa aaaattgaat aaacaaccga
    25621 gttcgctttc accgctggtg caattggcgg gaattcgcaa atgctttgat ggtaaagagg
    25681 tcattcccca gctggatctg actatcaaca atggcgagtt cctcacgctg cttggccctt
    25741 ctggctgcgg taaaacaacc gttcttcgcc tgattgcagg tctggaaact gttgattccg
    25801 gacgcatcat gctggataac gaggacatca cccacgttcc ggcggaaaac cgctatgtga
    25861 acactgtttt ccaaagctat gcacttttcc cccacatgac cgtgttcgaa aatgtggcct
    25921 ttgggttgcg catgcaaaaa acccccgctg ctgaaattac gccccgcgtg atggaagccc
    25981 tgcggatggt gcagctggaa accttcgctc aacgtaaacc gcatcagctc tctggtggtc
    26041 aacagcaacg cgtcgccatt gctcgagcgg tggttaacaa gcctcgtctg ttgttgctgg
    26101 atgagtcgct ctcagcgctg gattacaaac tgcgtaagca aatgcagaac gagctgaaag
    26161 cgttacagcg taagcttggc attacattcg ttttcgtgac tcacgaccag gaagaagcac
    26221 tcaccatgtc ggacaggatt gtggtgatgc gcgatggtcg cattgagcaa gacggcacgc
    26281 cgcgtgaaat ctacgaagag ccgaaaaacc tgtttgttgc cgggttcatt ggcgaaatta
    26341 atatgtttaa cgccacggtg atcgagcgac tcgacgagca gcgcgtacgc gccaacgttg
    26401 aaggccgcga atgtaatatc tacgttaact tcgccgttga accggggcaa aaactgcatg
    26461 ttctgctgcg cccggaagac ttacgtgttg aagagattaa cgacgacaac cacgctgaag
    26521 ggctgattgg ttacgttcgt gagcgtaact acaaaggcat gacgctggag tcggttgttg
    26581 aactggaaaa tggcaagatg gtgatggtca gcgaattctt caatgaagac gatcctgact
    26641 ttgaccactc tctcgaccaa aaaatggcca ttaattgggt agaaagctgg gaggtcgtac
    26701 tggctgatga agaacacaag taagttccag aatgtagtga ttgtcactat tgtcggttgg
    26761 cttgtgttgt ttgtctttct gcccaacctg atgatcattg gcaccagctt tttgacccgc
    26821 gacgacgcca gtttcgtcaa aatggtcttt acactggata actacacgcg tctgctcgat
    26881 ccgctctatt ttgaagtgct attgcactcg ctgaatatgg cgctgatcgc caccctcgcc
    26941 tgcctggtgc tgggctatcc atttgcctgg tttctggcga agctgccaca caaggtgcgt
    27001 ccgctgctgc tgtttctgct gattgttccg ttctggacca actcattgat tcgtatctac
    27061 gggctgaaaa ttttcctcag caccaaaggc tatctcaacg agtttttgct ctggctgggc
    27121 gttatcgaca caccaatccg tatcatgttc acgcccagtg cggtgattat cggtctggtt
    27181 tacattctgc tgccgtttat ggtgatgccg ctgtactcca gtatcgaaaa actggataag
    27241 ccgttgcttg aggcggcgcg cgatctcggt gccagcaagt tacagacttt tatccgcatc
    27301 attattccac tgacgatgcc ggggattatt gccggatgtc tgctggtgat gctgccagcg
    27361 atgggcctgt tctatgtgtc cgacctgatg ggcggtgcga aaaacctgct gatcggtaac
    27421 gtcatcaagg tacagttcct caatattcgt gactggccgt ttggtgcagc caccagcatt
    27481 acgctgacta tcgtaatggg cctgatgttg ctggtttact ggcgcgcttc tcgtttgctg
    27541 aataagaagg tggaactcga atgatcggtc gactgcttcg cggcggtttt atgaccgcta
    27601 tctacgcgta cctgtatatc ccaatcatta ttttgattgt gaactccttt aacagctcgc
    27661 gctttggcat caactggcag ggttttacca ccaaatggta tagcctgctg atgaacaacg
    27721 acagcctgtt acaggcagcg cagcattcac taacaatggc ggtgttttcg gcgacgtttg
    27781 ctacgcttat cggttcactg acggcagttg cgctgtaccg ttatcgcttt cgcggtaagc
    27841 cgttcgttag cggaatgctg tttgtggtaa tgatgtcacc agatatcgtg atggcaattt
    27901 cgctgctggt gctgtttatg ctgctgggta ttcagcttgg cttctggtcg ctgctgttct
    27961 cgcatatcac cttctgcctg ccatttgtgg tggtgacggt gtattcgcgc ctgaaaggtt
    28021 ttgacgtgcg gatgctggaa gcggcgaaag atctcggtgc cagcgaattt accattctgc
    28081 gaaaaatcat tctgccactg gcaatgccag cggtggcggc gggctgggtg ttaagcttta
    28141 ccctgtcgat ggacgatgtg gtagtttctt cattcgtcac cggaccaagt tatgaaattc
    28201 tgccgttaaa aatttattcg atggtcaaag tcggcgtatc gccggaagtt aacgcgctgg
    28261 caaccatatt actggtgctg tcgctggtga tggttattgc cagccagctt attgctcgtg
    28321 ataaaacgaa aggtaacaca ggggacgtta aatgaaaaaa tggtcacgcc acctgctcgc
    28381 ggcgggtgct ctggcactgg gcatgagcgc cgctcacgcc gatgacaaca acacgctgta
    28441 tttctacaac tggaccgagt acgtgccgcc aggactgctt gaacagttca ccaaagaaac
    28501 cggtattaag gttatctatt cgacttacga gtcgaacgaa accatgtacg ccaagctgaa
    28561 aacgtacaaa gacggtgcct atgacctggt ggttccttca acctattacg tcgataaaat
    28621 gcgtaaagag gggatgatcc agaagatcga caagtcgaag ttaacgaact tcagcaatct
    28681 cgatccagac atgctcaaca agccttttga cccgaataac gactactcca ttccgtatat
    28741 ctggggtgcg acggcgattg gtgttaacgg tgatgcggtg gatccgaaat ctgtcaccag
    28801 ctgggccgat ctgtggaagc cagagtacaa aggcagcctg ctgttgaccg acgatgcccg
    28861 tgaagtgttc cagatggcgc tgcgtaagct gggctactcc ggtaacacca ccgatccgaa
    28921 agagattgaa gctgcatata acgagctgaa aaaactgatg ccaaacgtcg cagcgtttaa
    28981 ctccgataac ccggctaacc cgtacatgga aggcgaagtt aacctcggca tgatctggaa
    29041 cggttctgct ttcgttgcac gccaggcggg tacgcctatt gacgtggtgt ggccgaaaga
    29101 aggcggcata ttctggatgg acagcctggc gatcccggca aatgccaaaa acaaagaagg
    29161 cgcgctgaaa ttgatcaact tcctgctgcg cccggatgtg gcaaaacagg ttgctgaaac
    29221 tatcggttat ccaacgccaa accttgcggc gcgtaagctg ttaagtccag aagtggcaaa
    29281 cgataagacg ctctacccgg atgctgaaac cattaaaaat ggcgaatggc agaatgacgt
    29341 tggcgcagcc agcagcattt atgaagagta ttatcagaag ctgaaagcag gacgttaatt
    29401 cagcaagcgg gcggtggtat taccgcccgt tttttagtta gcaaggatgt ggctaatgag
    29461 tcaggacagt aaagttttat cccgctcttt cgtcggtatt gtggtccgct tgtaggcctg
    29521 ataagcgtag cgcatcaggc aatgcttcct gctttataat ccctccagca acttttcaac
    29581 aaattctggc accacctggc ttgccgggcc gtaatatttc tcggcaaatt cattaccaac
    29641 ctgactcggt tcaagattca gttcaacggt gtgagcacca tgtagtttgg cttcgtgtac
    29701 aaacccagcc gccggataga catgcccgga agtaccaatg gcaataaaga catccgccat
    29761 cgacaacgcc atataaattt catccatgcc gagtggcatt tcgccaaacc aaacgacgtg
    29821 cgggcgcaag ggtgccggga actggcaaca atggcattta tcttctggcg taacgtctcc
    29881 ggtccagtcg agaacctgac cactttgcga acaacgaact ttcagcaatt ccccatgcat
    29941 atgaatgaca ttggtattac ctgcgcgttc atgcaggttg tctatattct gcgttaccag
    30001 caaaaagcga tcgccgagag cgtcttgcag tttagccagc gcaagatgcg cggcgttagg
    30061 ctgaatttct ggctgctgca gctgtcgacg acgggcgtta taaaacgctt gcaccagttc
    30121 aggatcgcga tcgaaacctt ccggcgttgc cacatcttca acccgatgtt cttcccacag
    30181 gccatctgcg gcgcgaaagg tacgaatacc tgattccgca gaaattcctg cccctgtcag
    30241 tacgagtact cttggttttt ccattgcttc cggcaccact ttatctctga aaaaaatacg
    30301 ctgacgcaaa cgctcgcgca ggcgacgttt atttttacga aaacgactta accgatgacc
    30361 ccgacgcgac agcatagcaa cctctgttgt ttaatcggtt agatgtagga aggccgcacc
    30421 acgcattcct cccgcatcac cgtggcgcgc gcgttcaatg cgtggaacac gagctacagg
    30481 taagagatga caaggcaacc tgtccgccag ttgcgttgtg attgccggga aattcgataa
    30541 gccaccacca atgacgacca ggtcagggtc aacaatggtc aggatatttc ccagagaaac
    30601 cgctaataaa tccagataac gctcaacgtg cgcccttgcc tgctcatcgc cttgatcata
    30661 aagcgcaata atttcgggag cctgcaacgg ttgatgataa tagtgttgat acagccacgc
    30721 aaaaccgcga ccagacagat aattttcaat gcagccatgc tgaccacagc cgcagcggcg
    30781 taacgggaaa tccaacccca tcatggttaa cgcatcaacc ggcagacgca tatggccaaa
    30841 ctcgccggta atgtagcttt taccagtaat cggtttgccg ttgaaaacca gcccgccgcc
    30901 aacgccggtg ccgagaatta accccatcac cagtggatat tgcgtaaatt catcatccca
    30961 ggcttctgaa agggcaaaac agttggcatc gttatcaagg cgtacatcgc gatcaagacg
    31021 tgcgctcagg tcggcacgca gcggtttgcc gctggcagcg gggacattag cggcatacag
    31081 cttaccatct tctgtttccg gcattcccgg aataccgatg ccgacagaac ctttacagcc
    31141 aaaacgttgg tcagcttcag ctaccagctc acacactgca tctaaaaatg cgtcatagct
    31201 gtcacgcggt gtcggcaccc gcttttccca ctgcaactgc cgaccgctat caaacacgcc
    31261 aagcgcaatt tttgttccac caatatcaaa cccgtaatac attgccgctc cttgttttaa
    31321 tgtactgcct ttactggccg ctaaggactc gcgcagggtc aatattactg gcgcgccgcg
    31381 ccggatacca acttgccaaa agactcagca acaatgctgt gaccagtacg tagaagacgt
    31441 ccagccagtg caattccgat ggcaggaagt caataaaata aatatcgctg gagaggaact
    31501 gatgaccgat cagcttttca atccactcaa taatcggggt aagttgcagt gaaacaacta
    31561 cgccgataac caccccacac aggctgccga acagccctgc cagcaatcca taccagacaa
    31621 agatggcgcg aattaaacca tctttcgccc ccagcgttct taatactgcg atatcgccac
    31681 tcttgtcttt caccgccatc actaaggtgg agacgatgtt gaaacaggcc acgccaatca
    31741 ccagtaccat cgccagatac ataatggcgc ggatcatctg gatatcgcga tacatatagc
    31801 cgtaagtacc aatccaactt ttaatataaa catagctgtt ggtcacttca cccgcatcgc
    31861 gtaccagctt attggcgttg aaaacatccg tcattttaag ggcaatacct gacacgctgg
    31921 aacccatatc aagatattgt tgggcatccg ccagcgggat catggcaaaa ctgtgatcga
    31981 gttgaccact caactgcaaa ataccggcaa tgtgcaaacg cacacgtttt ggctgcatca
    32041 gtttatgctc gggattcgag ttggggatca tgatcgacac ccaatcgccc tgcttcactt
    32101 tcagcgcatc cgccacgcct ttgccgatga taatttgctg ttcgcccgct ttaaaattgc
    32161 gccacgcatc cccctgaaca aacgagggta atgcgctcag acgctgttcc tgttgtgggt
    32221 taacgccctt cacctggatt gcgcgcagat tcgctccact ttccaccaga ccggtgaaat
    32281 tgatatacgg cgcagcggcg gcaatacccg gcactttctg cacgttatcc agtgcttcct
    32341 gccagttagt ccatggctga tttaccgctt cgatttcgcc atgcggcacc accgccagaa
    32401 tgcggttatt cagttcgcgt tcaaagccgt tcatcgcgct taagccgacg atcaataccg
    32461 ccacgccgag ggcaatgcca atggtagaaa tgacagagat tagcgacacc atgccgccgc
    32521 gtcgccgtcc gcgactaaaa cgcaggccaa tcaataacga taaaggcatc gccattactc
    32581 cgcccccatc aggctcagtt ccgccgtcag acgtccatca cgcatttcca gttggcggct
    32641 catacgtttc gccagttgca ggtcgtgagt gaccaccagg aaagcggtgc cctgcaagcg
    32701 attcaactcc ccaagcaact ggaagatgct gtcggcgtta cgcgcatcga ggttaccggt
    32761 aggttcatcc gccagtacca gacgcggatt attgaccagc gcacgggcaa tcgctacacg
    32821 ctggcgttcg ccgccagaaa gttcagatgg gcggtgattc gcacgatgct ccagccccac
    32881 cgcttttaac atctccagtg cacagctgtt gatttcagcg ggctttttct tgccaatcag
    32941 cagtggcata gccacgtttt ccagggcagt aaaatccggc agcaggtggt gaaactgata
    33001 aataaagccc agcttctggt tgcgcagttc tgctttcgcc gctgaagaca gtttgctcat
    33061 cggctgaccg ttaaagatca catcgccgga ggttggcgta tccagcccgc ctaacaggtg
    33121 cagcaaggta cttttaccgg aaccagagct accgacaatt gccatcattt caccttcgcc
    33181 cacgctgaaa ctgacattga gcagcacatc ggtttgcaca ctgccttcct gatagcgttt
    33241 gcacaggttg tcgcattgca acaggatctt attcataacg taaagcctca gcgggttgtg
    33301 tggcggcagc gcgccatgaa gggtaaagcg tagacagcag cgcgatagcc atcgccacca
    33361 gcgcaataac aatgacctgt aaaggttcga tagccaccgg cagcgccgcg ccatcaagca
    33421 ggacgccgat tatcggcatc agattattta actggctggc aagcagtgcg ccaagcgccg
    33481 ctccgaggat tgcaccgata atcccggcgc tagccccttg caccataaag accatcatga
    33541 tttgtcgcgg agttaagcct tgcgtttgca ggatcgcgac ttcgccctgc ttctccatca
    33601 ccatcagccc cagcgaggta ataatgttaa acgccgcaac agcgacaatc aggctcagca
    33661 gtaagcccat catatttttt tccatgcgta cggcctggaa cagctcgcct ttacggtcac
    33721 gccagtcctg ccatttgctg ccttcaggca gtttttgctg acttaaagag tcaactctca
    33781 gcggctcatc cagccacaaa cgccagccgg taatattggc tgccggataa cgcatcagac
    33841 gcgaggcatc ctcaatattc accagcattt catagccatc gacttcactg ttggcggcga
    33901 aagtgccaat cacattgaac aggcgctgac ttggaatacg ccccatcggc gtgaactggc
    33961 tggcagatgg caccatcacg cggatttgat caccgcgatt gacgcccaac tgtgaggcaa
    34021 gttgttcgcc gaggatgaca ttatatttcc ccggctcgag gtcagtttgt ttcacattga
    34081 ccagatacgg cgttagtgga tctttttgcg ccgggtcgat accgagcatc accccgaccg
    34141 ccacgctgcg cgcgctttgc agtaccacat caccggtagt aataggtgcg acgcgattaa
    34201 cgccgtccag tttgactgcc gtttccggga gttgctgcgg gttaagagag ccatgctcag
    34261 aagagagaat tgcctgtggc atcaggccaa ggatgttgtt ttgcagctcg cgctcaaagc
    34321 cgttcatcac tgacaatact gtgaccagcg ccatcacccc gagggtaatg ccgatggtag
    34381 aaagccagga gacgaaacga ccgaagcgat ccactgcacg cccacgcatg taacgcaggc
    34441 caataaatag agcgacaggt tggtacatga aatccgtctg gttgctgtag caaagacacg
    34501 agtatataag cgaaagcaag atgggtaaac gtctgaactg taagtgatcg tcagggctaa
    34561 tctgaaaagc ccccgtaaat caatcggcta aattgccata ttttatcatc aatcttgccg
    34621 cctgatctta atctgaaaaa tcatccaata cagacagata cccattacac ctcgggtcat
    34681 aaccagcagg atcaaccgca tgtaaattaa aaaagagcgt gttgttctga tgaaacaaaa
    34741 agagctatgg attaaccaga tcaaagggtt atgtatttgt ctggtggtga tttatcactc
    34801 ggtcattacc ttttatccgc atatgaccac ttttcagcat ccgttatcgg aagtcctgag
    34861 caaatgctgg atctatttca atctttacct tgcccccttt cgtatgccgg tttttttctt
    34921 tatatccggc tatttgattc gccgctatat cgacagcgta ccgtggggaa attgtctcga
    34981 taaacggatc tggaacatct tctgggtgct ggcgctttgg ggcgtggtgc agtggctggc
    35041 gttaagtgcg ctgaaccagt ggctggcacc tgagcgcgat ttaagcaatg cctccaatgc
    35101 cgcttatgcc gattccaccg gtgagttcct gcacgggatg atcaccgcca gcaccagctt
    35161 gtggtatctg tatgcgttaa ttgtctattt cgtggtatgt aaaattttta gccgtctggc
    35221 gctgccacta ttcgccttgt ttgtactgct gagtgtggcg gttaatttcg ttcccacgcc
    35281 gtggtgggga atgaacagtg tgatacgcaa tttgccttat tacagccttg gcgcatggtt
    35341 tggcgcaaca ataatgacct gtgttaaaga ggtgccgttg cgccgccatc tgctgatggc
    35401 ttctttgctt accgttctgg cggtcggtgc ctggttgttt actatctcgc tgctgttgtc
    35461 gctggtatcg attgtggtga tcatgaagct gttttatcag tacgagcaac gattcggtat
    35521 gcgctccacc agcctgctga atgtgattgg ctccaacacc attgctatct acaccaccca
    35581 tcgcattctg gttgaaatat tcagcttaac tctgcttgcg caaatgaacg cagcacgctg
    35641 gtcgccgcaa gtcgaactga cactcctgct ggtttatccc tttgtcagtt tgttcatctg
    35701 tactgttgcg ggcttgctgg taagaaaact ttcacagcgt gcattcagcg atctgttgtt
    35761 ctccccgcct tcactgcccg ctgccgtcag ttactctcgt taaaccatca ccccagccgg
    35821 ggcgattcaa tttgctaaac catgtcgatt acggataatg aacagcattg cttatcagag
    35881 atatgccccc atatgttgag gcatatccta acgagaatct gacaaccgtt atgcctgaac
    35941 aatatcgtta tacgctgccc gtcaaagcgg gtgagcagcg tctgctgggc gagttaaccg
    36001 gcgcagcctg tgcaacgctg gtagcggaaa ttgccgaacg tcacgccggt ccggtggtac
    36061 tcattgcacc agatatgcaa aatgctctgc gtttgcatga tgaaatcagc cagttcaccg
    36121 atcagatggt gatgaatctg gcggactggg aaactcttcc ctacgacagt ttttcgcctc
    36181 atcaggacat tatctcctcg cgcctttcca ccctttacca gctaccgacg atgcagcgtg
    36241 gcatactgat tgttccggtg aatacgctta tgcagcgcgt ttgcccacac agttttctcc
    36301 acggtcatgc gctggtgatg aaaaaaggtc agcgcctgtc acgagatgca ttacgaaccc
    36361 aactggacag cgccggttat cgccatgttg accaggtgat ggagcacggc gaatacgcca
    36421 cgcgcggcgc gttgctggat ctcttcccga tggggagtga gctgccttat cgtcttgatt
    36481 tctttgatga tgaaatcgac agcctgcggg tgtttgacgt cgacagccag cgcacgctgg
    36541 aggaagtaga agcgatcaat ctgctgcccg cgcacgaatt tccgaccgat aaagcggcaa
    36601 ttgaactgtt ccgcagccag tggcgcgata ccttcgaagt gaagcgcgat ccggaacata
    36661 tttaccagca agtgagtaaa ggcacattac ctgctgggat cgagtactgg cagccattgt
    36721 tcttcagcga accactgcca ccgctgttca gttatttccc tgccaatacc ttgctcgtga
    36781 atactggcga tctggaaacc agtgccgaac gtttccaggc tgacacgctg gcgcgttttg
    36841 agaatcgcgg cgtcgatccg atgcgcccgc tgttgccacc acaatcgctc tggctgcggg
    36901 tggacgagct cttctcagag ctgaaaaact ggccccgagt gcagctaaaa actgaacatt
    36961 taccgacaaa agccgcgaat gccaatttag gtttccagaa actgccagac ctggccgttc
    37021 aggcgcaaca aaaagcgccg ctggatgcgc tgcgtaagtt cctcgagact ttcgacggtc
    37081 cggtggtgtt ctcggtagaa agtgaaggtc gccgtgaagc gctgggtgaa ctgctcgcac
    37141 gaattaaaat tgctccgcaa cgcattatgc gtcttgatga agccagcgac cgtgggcgtt
    37201 atctgatgat tggcgctgcc gaacatggtt ttgtcgataa ggcgcgtaat ctggcgctga
    37261 tctgcgaaag cgatctgctc ggtgaacgtg ttgcccgccg tcgtcaggat tctcgccgca
    37321 ccatcaaccc cgatacactg atccgtaacc ttgcggagct gcatattggt cagccggtgg
    37381 tccatctgga gcacggcgtc ggtcgctatg ccggaatgac cacgctggaa gctggcggca
    37441 ttactggcga gtacctgatg ctcacctatg ccaacgacgc caaactgtat gttccggtgt
    37501 cgtcactgca tctgattagc cgttacgcag gtggcgcgga agaaaacgcc ccgctgcata
    37561 aacttggcgg cgatgcgtgg tcacgcgcgc ggcagaaagc ggcggaaaaa gtgcgtgatg
    37621 tggcggcgga attgctggat atctacgcgc aacgcgccgc caaagagggc ttcgcgttta
    37681 aacacgatcg tgagcagtat cagttattct gcgacagctt cccgtttgaa accacgccgg
    37741 atcaggcgca ggccattaat gcggtactta gcgacatgtg tcagccgctg gcaatggatc
    37801 gtctggtgtg cggcgatgtt ggctttggta aaacagaagt ggcgatgcgc gccgctttcc
    37861 tggcagtaga taaccacaag caggtagcgg tgttggtgcc taccaccctt cttgcgcagc
    37921 agcattacga caacttccgc gaccgtttcg ccaactggcc ggtacgtatc gaaatgatct
    37981 cccgtttccg cagcgccaaa gagcagacgc aaatccttgc ggaagtggcg gaagggaaaa
    38041 tcgatattct gatcggtacg cacaaactgc tgcaaagtga cgtcaagttt aaagatttag
    38101 gcctgctgat tgtcgatgaa gaacaccgct tcggggtgcg tcataaagag cgcattaaag
    38161 cgatgcgcgc gaacgtggat attctgacgc ttactgcaac gccgatccca cgtacgctga
    38221 atatggcaat gagcggaatg cgtgacctgt cgattatcgc cacgccgccc gcccgtcgtc
    38281 tggcagttaa aacctttgtc cgtgagtatg acagcctggt ggtccgggag gcgatcctgc
    38341 gtgaaatttt gcgcggagga caggtttatt atctctacaa tgatgtggaa aacattcaga
    38401 aagccgccga acggctggca gaactggtgc ctgaagcacg gattgccatc ggtcacgggc
    38461 agatgcgcga gcgcgaactg gaacgggtga tgaatgattt ccatcatcaa cgtttcaacg
    38521 tgctggtttg taccaccatt atcgaaaccg ggatcgacat cccgacagcc aacaccatta
    38581 tcattgaacg tgcggatcac ttcggtctgg cgcagctgca ccagttacgc ggtcgcgtcg
    38641 gacgttcaca tcatcaggca tatgcatggc tgctgacgcc gcatccaaaa gcgatgacta
    38701 ccgatgcaca aaaacgtctt gaagcgattg cctcgctgga agatctcggg gcaggttttg
    38761 cgctggcaac gcacgatctg gagattcgcg gcgcgggtga actgcttggc gaagaacaaa
    38821 gcggctcaat ggaaaccatc ggtttctcgc tgtatatgga gttgctggaa aacgccgtcg
    38881 atgcactgaa agccggacgc gagccgtcgc tggaagatct caccagccag caaacagaag
    38941 tcgagctgcg gatgccgtcg ctattgccag atgatttcat ccctgacgtg aacacgcgtc
    39001 tgtcgttcta caaacgtatt gccagcgcca aaacggaaaa cgaactggaa gagatcaaag
    39061 tcgagcttat cgatcgcttc ggcctgctgc cagatccggc gcgtaccctg ctggatattg
    39121 cccgtctgcg ccagcaagcg cagaaactgg ggatcaggaa gctggaaggt aatgagaaag
    39181 gcggggtgat cgaatttgcc gagaagaatc acgttaatcc ggcctggttg attggtttgc
    39241 tgcaaaaaca gccgcagcat taccgccttg atggtccgac gcgcctgaaa tttattcagg
    39301 atttgagtga gcggaaaacg cgtatcgaat gggtacgcca gtttatgcgt gaactggaag
    39361 agaacgcgat cgcttaatgt aaatcggggg cgtttctgcg cccccatctg caacatttac
    39421 aaattctttg cactttcctg cactatccga cactgtcacc atccataatt caggcttatc
    39481 tgtttattac aataacctta tatttattat gtatttttgg tgatgatcaa aacgcgtttt
    39541 tctcgctggc taacgttttt tacgttcgcc gctgccgtgg cgctggcgct accggcaaaa
    39601 gccaacacct ggccgctgcc gccagcgagc agtcgtctgg ttggcgaaaa caaatttcat
    39661 gtggtggaaa atgacggtgg ttctctggaa gccatcgcca aaaaatataa cgtcggcttt
    39721 ctcgctctgt tacaagctaa ccccggcgtt gatccttacg taccgcgcgc gggcagcgtg
    39781 ttaacgatcc cgttgcaaac cctacttcca gacgcgccgc gcgaaggcat tgtgatcaac
    39841 attgcggagc tgcgtctcta ttactacccg ccgggtaaaa attcggtaac cgtgtatcca
    39901 ataggtattg gtcagttagg tggtgacacg ctgacaccga caatggtgac caccgtttca
    39961 gacaaacgtg caaacccaac ctggacgcca acggcaaaca tccgcgcccg ttataaagca
    40021 cagggaattg agttgcctgc ggtagtgccg gctggaccgg ataacccaat gggccatcat
    40081 gcgattcgtc tggcggccta tggcggcgtt tatttgcttc acggtacgaa cgccgatttc
    40141 ggcattggca tgcgggtaag ttctggctgt attcgtctgc gggatgacga tatcaaaaca
    40201 ctctttagcc aggtcacccc aggcaccaaa gtgaatatca tcaacactcc gataaaagtc
    40261 tctgccgaac caaacggtgc gcgtctggtt gaagtacatc agccgctgtc agagaagatt
    40321 gatgacgatc cgcagctgct gccaattacg ctgaatagcg caatgcaatc atttaaagat
    40381 gcagcacaaa ctgacgctga agtgatgcaa catgtgatgg atgtccgttc cgggatgccg
    40441 gtggatgtcc gccgtcatca agtgagccca caaactctgt aaatcctgga taaaaaaaat
    40501 ccccgcgaca cggcatgtcg cggggaaaaa atcagcagta gcaggcatta atgagggtta
    40561 atgcttattt ataaattact gctgttccat ggagggtatt cggaccggtt acagaagtaa
    40621 tacggaaaga ttttgcgccc atctcatccg ctttttgcgc cagctgctct tccagcgatc
    40681 ccagatttgt ccccgcgtta gcactgatag taccgacttt ttgttggcct tctggcgttg
    40741 actgaacttc gacagccgca aagttggcaa atgacatgga gcttaaaatc gccgcagcga
    40801 tgaggttttt acgtttttca taatagtggc cttatgcaga tgaatgacgt cgcaagaaat
    40861 tacttaacga tcgataacta aatgatagat g
//
LOCUS       DAZPCS010000020        40243 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZPCS010000020 DAZPCS010000000
VERSION     DAZPCS010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40243
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.020"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            324..410
                     /gene="leuL"
                     /locus_tag="ACHUQ7_001043"
     CDS             324..410
                     /gene="leuL"
                     /locus_tag="ACHUQ7_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFR1134558.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            503..2074
                     /gene="leuA"
                     /locus_tag="ACHUQ7_001044"
     CDS             503..2074
                     /gene="leuA"
                     /locus_tag="ACHUQ7_001044"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFR1134559.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVTNYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKTQHNENNKETV"
     gene            2074..3165
                     /gene="leuB"
                     /locus_tag="ACHUQ7_001045"
     CDS             2074..3165
                     /gene="leuB"
                     /locus_tag="ACHUQ7_001045"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFR1134560.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDIQCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3168..4568
                     /gene="leuC"
                     /locus_tag="ACHUQ7_001046"
     CDS             3168..4568
                     /gene="leuC"
                     /locus_tag="ACHUQ7_001046"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFR1134561.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAALVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4579..5184
                     /gene="leuD"
                     /locus_tag="ACHUQ7_001047"
     CDS             4579..5184
                     /gene="leuD"
                     /locus_tag="ACHUQ7_001047"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFR1134562.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(5233..6411)
                     /gene="setA"
                     /locus_tag="ACHUQ7_001048"
     CDS             complement(5233..6411)
                     /gene="setA"
                     /locus_tag="ACHUQ7_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HFR1134563.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLLSVARVELPSENALSMQGDWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVALFLTAKVKDV
                     "
     gene            complement(6513..6644)
                     /gene="sgrT"
                     /locus_tag="ACHUQ7_001049"
     CDS             complement(6513..6644)
                     /gene="sgrT"
                     /locus_tag="ACHUQ7_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFR1134564.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            6733..8388
                     /gene="sgrR"
                     /locus_tag="ACHUQ7_001050"
     CDS             6733..8388
                     /gene="sgrR"
                     /locus_tag="ACHUQ7_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFR1134565.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEKKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      8422..8521
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8552..9535
                     /gene="thiB"
                     /locus_tag="ACHUQ7_001051"
     CDS             8552..9535
                     /gene="thiB"
                     /locus_tag="ACHUQ7_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFR1134566.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EANCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            9511..11121
                     /gene="thiP"
                     /locus_tag="ACHUQ7_001052"
     CDS             9511..11121
                     /gene="thiP"
                     /locus_tag="ACHUQ7_001052"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFR1134567.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            11105..11803
                     /gene="thiQ"
                     /locus_tag="ACHUQ7_001053"
     CDS             11105..11803
                     /gene="thiQ"
                     /locus_tag="ACHUQ7_001053"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFR1134568.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(11917..12681)
                     /locus_tag="ACHUQ7_001054"
     CDS             complement(11917..12681)
                     /locus_tag="ACHUQ7_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR1134569.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(12767..13645)
                     /gene="araC"
                     /locus_tag="ACHUQ7_001055"
     CDS             complement(12767..13645)
                     /gene="araC"
                     /locus_tag="ACHUQ7_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFR1134570.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPYFSDLFGQIINAGQGEGRYSELLAMNLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            13984..15684
                     /gene="araB"
                     /locus_tag="ACHUQ7_001056"
     CDS             13984..15684
                     /gene="araB"
                     /locus_tag="ACHUQ7_001056"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFR1134571.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDTPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            15695..17197
                     /gene="araA"
                     /locus_tag="ACHUQ7_001057"
     CDS             15695..17197
                     /gene="araA"
                     /locus_tag="ACHUQ7_001057"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFR1134572.1"
                     /translation="MAIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PYKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGYLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            17397..18092
                     /gene="araD"
                     /locus_tag="ACHUQ7_001058"
     CDS             17397..18092
                     /gene="araD"
                     /locus_tag="ACHUQ7_001058"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFR1134573.1"
                     /translation="MLKDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLHKH
                     YLRKHGAKAYYGQ"
     gene            18167..20518
                     /gene="polB"
                     /locus_tag="ACHUQ7_001059"
     CDS             18167..20518
                     /gene="polB"
                     /locus_tag="ACHUQ7_001059"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFR1134574.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IHADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDKAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDIIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            20683..23589
                     /gene="rapA"
                     /locus_tag="ACHUQ7_001060"
     CDS             20683..23589
                     /gene="rapA"
                     /locus_tag="ACHUQ7_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFR1134575.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKSCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            23601..24260
                     /gene="rluA"
                     /locus_tag="ACHUQ7_001061"
     CDS             23601..24260
                     /gene="rluA"
                     /locus_tag="ACHUQ7_001061"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFR1134576.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(24377..25192)
                     /gene="djlA"
                     /locus_tag="ACHUQ7_001062"
     CDS             complement(24377..25192)
                     /gene="djlA"
                     /locus_tag="ACHUQ7_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFR1134577.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            25447..27801
                     /gene="lptD"
                     /locus_tag="ACHUQ7_001063"
     CDS             25447..27801
                     /gene="lptD"
                     /locus_tag="ACHUQ7_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFR1134578.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            27854..29140
                     /gene="surA"
                     /locus_tag="ACHUQ7_001064"
     CDS             27854..29140
                     /gene="surA"
                     /locus_tag="ACHUQ7_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFR1134579.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            29140..30129
                     /gene="pdxA"
                     /locus_tag="ACHUQ7_001065"
     CDS             29140..30129
                     /gene="pdxA"
                     /locus_tag="ACHUQ7_001065"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFR1134580.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            30126..30947
                     /gene="rsmA"
                     /locus_tag="ACHUQ7_001066"
     CDS             30126..30947
                     /gene="rsmA"
                     /locus_tag="ACHUQ7_001066"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFR1134581.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDIRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            30950..31327
                     /gene="apaG"
                     /locus_tag="ACHUQ7_001067"
     CDS             30950..31327
                     /gene="apaG"
                     /locus_tag="ACHUQ7_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFR1134582.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            31334..32176
                     /gene="apaH"
                     /locus_tag="ACHUQ7_001068"
     CDS             31334..32176
                     /gene="apaH"
                     /locus_tag="ACHUQ7_001068"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFR1134583.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(32254..32733)
                     /gene="folA"
                     /locus_tag="ACHUQ7_001069"
     CDS             complement(32254..32733)
                     /gene="folA"
                     /locus_tag="ACHUQ7_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFR1134584.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(32924..34786)
                     /gene="kefC"
                     /locus_tag="ACHUQ7_001070"
     CDS             complement(32924..34786)
                     /gene="kefC"
                     /locus_tag="ACHUQ7_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFR1134585.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWVKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(34779..35309)
                     /gene="kefF"
                     /locus_tag="ACHUQ7_001071"
     CDS             complement(34779..35309)
                     /gene="kefF"
                     /locus_tag="ACHUQ7_001071"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFR1134586.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(35357..36751)
                     /locus_tag="ACHUQ7_001072"
     CDS             complement(35357..36751)
                     /locus_tag="ACHUQ7_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1134587.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(36810..37097)
                     /gene="fixX"
                     /locus_tag="ACHUQ7_001073"
     CDS             complement(36810..37097)
                     /gene="fixX"
                     /locus_tag="ACHUQ7_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFR1134588.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(37094..38380)
                     /locus_tag="ACHUQ7_001074"
     CDS             complement(37094..38380)
                     /locus_tag="ACHUQ7_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR1134589.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVLLAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGNPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(38431..39372)
                     /locus_tag="ACHUQ7_001075"
                     /pseudo
     CDS             complement(38431..39372)
                     /locus_tag="ACHUQ7_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
     gene            complement(39387..40157)
                     /locus_tag="ACHUQ7_001076"
     CDS             complement(39387..40157)
                     /locus_tag="ACHUQ7_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFR1134590.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
BASE COUNT         9679 a        11120 c        10567 g         8877 t
ORIGIN      
        1 attcaccaaa tcaatatcaa aaaaaatcgc aaaacatata attcaataca aatcatcagg
       61 ataggttttg caacgcgtgc attttgtccc ctttttcctc gttgattaga tgcaaaaatt
      121 tatgctgaaa tatgtcaacc gatgaaaagc gtcggtagtt aagcagaaat taatatcgct
      181 tactttaacc accgtagcac aattagctaa ttttacggat gcagaactca cgctggcggg
      241 acgtttttat tgcgtcaggg ttgacatccg tttttgtatc cagtaactct aaaagcatat
      301 cgcattcatc tggagctgat ttaatgactc acatcgttcg ctttatcggt ctactactac
      361 taaacgcatc ttctttgcgc ggtagacgag tgagcggcat ccagcattaa gccagcacgc
      421 agtcaaacaa aaaacccgcg ccattgcgcg ggttttttta tgcccgaagc gaggcgctct
      481 aaaagagaca aggacccaaa ccatgagcca gcaagtcatt attttcgata ccacattgcg
      541 cgacggtgaa caggcgttac aggcaagctt gagtgtgaaa gaaaaactgc aaattgcgct
      601 ggcccttgag cgtatgggtg ttgacgtgat ggaagtcggt ttccccgtct cttcgccggg
      661 cgattttgaa tcggtgcaaa ccatcgcccg ccaggttaaa aacagccgcg tatgtgcgtt
      721 agctcgctgc gtggaaaaag atatcgacgt ggcggccgaa tccctgaaag tcgccgaagc
      781 cttccgtatt cataccttta ttgctacctc cccaatgcac atcgccacca agctgcgcag
      841 caccctagac gaggtgatcg aacgcgctat ctatatggtg aaacgcgccc gtaattacac
      901 cgatgatgtt gaattttctt gcgaagatgc cgggcgtaca cccattgccg atctggcgcg
      961 agtggtcgaa gcggcgatta atgccggtgc caccaccatc aacattccgg acaccgtggg
     1021 ctacaccatg ccgtttgagt tcgccggaat catcagcggc ctgtatgaac gcgtacctaa
     1081 catcgacaaa gccattattt ccgtacatac ccacgacgat ttaggcctgg cggtcggcaa
     1141 ctcactggcg gcggtacatg ccggtgcacg ccaggtggaa ggtgcaatga acgggatcgg
     1201 cgagcgtgcc ggtaactgtt cgctggaaga agtcatcatg gcgattaaag ttcgtaagga
     1261 tattctcaac gtccacaccg ccattaatca ccaagagata tggcgcacca gccagttagt
     1321 tagccagatt tgtaatatgc cgatcccggc aaacaaagcc attgttggca gcggcgcatt
     1381 cgctcactcc tccggtatcc accaggatgg cgtgctgaaa aaccgcgaaa actacgaaat
     1441 catgacacca gaatctattg gtctgaacca aatccagctg aatctgacct ctcgttcggg
     1501 gcgtgcggcg gtgaaacatc gcatggatga gatggggtat aaagaaagtg aatataattt
     1561 agacaatttg tacgatgctt tcctgaagct ggcggacaaa aaaggtcagg tgtttgatta
     1621 cgatctggag gcgctggcct tcatcggtaa gcagcaagaa gagccggagc atttccgtct
     1681 ggattacttc agcgtgcagt ctggctctaa cgatatcgcc accgccgccg tcaaactggc
     1741 ctgtggcgaa gaagtcaaag cagaagccgc caacggtaac ggtccggtcg atgccgtcta
     1801 tcaggcaatt aaccgcatca ctgactataa cgtcgaactg gtgaaataca gcctgaccgc
     1861 caaaggtcac ggtaaagatg cgctgggtca ggtggatatt gtcaccaact acaacggtcg
     1921 ccgcttccac ggcgtcggcc tggccaccga tattgtcgag tcctccgcca aagccatggt
     1981 gcacgtactg aacaatatct ggcgtgccgc agaagtcgaa aaagagttgc aacgcaaaac
     2041 tcaacacaac gaaaacaaca aggaaaccgt gtgatgtcga agaattacca tattgccgta
     2101 ttgccgggag acggtattgg tccggaagtg atgacccagg cgctgaaagt gctgaatgcc
     2161 gtgcgcaacc gctttgcgat gcgcatcacc accagccatt acgatgtagg cggcgcagcc
     2221 attgataacc acgggcaacc actgccgcct gcgacggttg aaggttgtga gcaagccgat
     2281 gccgtgctgt ttggctcggt aggcggcccg aagtgggaac atttaccacc agaccagcaa
     2341 ccagaacgcg gcgcgctgct gccgctgcgt aagcacttca aattattcag caacctgcgc
     2401 ccggcaaaac tgtatcaggg gctggaagca ttctgtccgc tgcgtgcaga cattgccgct
     2461 aacggcttcg acatccagtg cgtgcgcgaa ctgaccggcg gcatctattt cggtcagcca
     2521 aaaggccgcg aaggtagcgg acaatatgaa aaagcgtttg ataccgaggt gtatcaccgt
     2581 tttgagatcg agcgtattgc ccgcatcgcg tttgaatctg ctcgtaaacg tcgccacaaa
     2641 gtcacctcaa tcgacaaagc taacgtgctg caatcctcta ttttatggcg tgaaatcgtc
     2701 aacgagatcg ccacggaata cccggatgtc gaactggcgc atatgtacat cgacaacgcc
     2761 accatgcagc tgattaaaga tccatcacag tttgacgttc tgctgtgctc caacctgttt
     2821 ggcgacattc tgtctgacga gtgcgcaatg atcactggct cgatgggcat gttgccttcc
     2881 gccagcctga acgagcaagg ttttggactg tatgaaccgg cgggcggctc ggcaccagat
     2941 atcgcaggca aaaacatcgc caacccgatt gcacaaatcc tgtcgctggc actgctgctg
     3001 cgttacagcc tggatgccga tgatgcggct tccgccattg aacgcgccat taaccgtgca
     3061 ttagaagaag gcattcgcac cggggattta gcccgtggcg ctgccgccgt tagtaccgat
     3121 gaaatgggcg atatcattgc ccgctatgtg gcagaagggg tgtaatcatg gctaaaacgt
     3181 tatacgaaaa attattcgac gctcacgtag tgtacgaagc cgaaaacgaa accccgctgt
     3241 tatatatcga ccgccacctg gtgcatgaag tgacctcacc gcaggcgttc gatggtctgc
     3301 gcgcccacgg tcgcccggta cgtcagccgg gcaaaacctt cgccactatg gatcacaacg
     3361 tctctaccca gaccaaagac attaatgcct gtggtgaaat ggcgcgcatc cagatgcagg
     3421 agctgattaa aaactgcaaa gaatttggcg tcgagctgta tgacctgaat cacccgtatc
     3481 aggggatcgt ccacgtaatg gggccggaac agggcgtcac cttgccgggg atgaccattg
     3541 tctgcggcga ctcgcatacc gccacccacg gcgcgtttgg cgcactggcc tttggtatcg
     3601 gcacttccga agttgaacac gtactggcaa cacaaaccct gaaacagggc cgtgcgaaga
     3661 ccatgaaaat tgaagtccag ggcaaagccg cgccgggcat taccgcaaaa gatatcgtgc
     3721 tggcaattat cggtaaaact ggcagcgcag gcggcaccgg gcatgtggtg gagttttgcg
     3781 gcgaagcaat tcgtgattta agcatggaag gtcgtatgac cctgtgcaat atggcgatcg
     3841 aaatgggcgc aaaagccgct ctggttgctc ctgacgaaac cacctttaac tatgtcaaag
     3901 gccgtctgca tgcgccgaaa ggcaaagatt tcgacgacgc cgttgcctac tggaaaaccc
     3961 tgcaaaccga cgaaggcgca actttcgata ccgttgtcac tctgcaagca gaagaaattt
     4021 caccgcaggt cacctggggc accaatcccg gccaggtgat ttctgtgaac gacaatattc
     4081 ccgatccggc ttcgtttgcc gatccggttg aacgtgcgtc ggcagaaaaa gcgctggcct
     4141 atatggggct gaaaccgggt attccgctga ccgaagtggc tatcgacaaa gtgtttatcg
     4201 gttcctgtac caactctcgc attgaagatt tacgcgcggc agcggagatc gccaaagggc
     4261 gaaaagtcgc gccaggcgtg caggcactgg tggttcccgg ctctggcccg gtaaaagccc
     4321 aggcggaagc ggaaggtctg gataaaatat ttattgaagc cggttttgaa tggcgcttgc
     4381 ctggctgctc aatgtgtctg gcgatgaaca acgaccgtct gaatccgggc gaacgttgtg
     4441 catccaccag caaccgtaac tttgaaggcc gccaggggcg cggcgggcgc acgcatctgg
     4501 tcagcccggc aatggctgcc gctgctgctg tgaccggaca tttcgccgac attcgcaaca
     4561 ttaaataagg agcacaccat ggcagagaaa tttatcaaac acacaggcct ggtggttccg
     4621 ctggatgccg ccaatgtcga taccgatgca atcatcccga aacagttttt gcagaaagtg
     4681 acccgtacgg gttttggcgc gcatctgttt aacgactggc gttttctcga tgaaaaaggc
     4741 caacagccaa acccggactt cgtgctgaac ttcccgcagt atcagggcgc ttccattttg
     4801 ctggcacgag aaaacttcgg ctgtggctcc tcgcgtgagc acgcgccctg ggcattgacc
     4861 gactacggtt ttaaagtggt gattgcgccg agttttgctg acatcttcta cggcaatagc
     4921 tttaacaacc agctgctgcc ggtgaaatta agcgatgcag aagtggacga actgtttgcg
     4981 ctggtgaaag ccaatccggg gatccatttc gacgtggatc tggaagcgca agaggtgaaa
     5041 gcgggagaga aaacctatcg ctttaccatc gatgccttcc gccgccactg catgatgaat
     5101 ggtctggaca gcatcggcct gaccctgcag cacgacgacg ctattgcgtc ttatgaagca
     5161 aaacaacctg cgtttatgaa ttagacccct tgcccggtca aatgaccggg ctttccgcta
     5221 tcgtccacgt catcaaacgt ctttaacctt tgcggttaaa aataatgcga caacagaaat
     5281 aatcgcaatt acccagtaga cagcaaagtg cccccaactt tgtgcaattg ctccctgaat
     5341 aacaccagcc agaattaccc cggtagagat agtgttggta aataaggtgg tagctgcccc
     5401 cgctcttcca ggcattaaat cctgaaacca tagcatccca atacccgcaa caatgccgat
     5461 aaatacagcg ttaaaaagtt gcagcgtcat caacgccata cggctatgaa agaaaatcaa
     5521 tccagtgtaa aacagcactc ctgccaccac tgcaatgacc atcattcgcc gcttaccata
     5581 acgtttgaca tagtagccag ccagaatcat tgctggtatt tccagtccag ctgccgtccc
     5641 catcaggaaa cccgccagtt tgtctggcaa tcctaaatcg ctactgatcc acaatggcat
     5701 atcaataatg tacatagtgt tgcaggtcca cattaacgtc gaggcgacaa ataacatccg
     5761 tacgttacta tcctgccagt caccttgcat tgataaagca ttttccgacg gcagttctac
     5821 ccgcgccaca gacagaagca taaatgcaat caataccaga ctgagtgtga atatcccggc
     5881 ggcaatcgaa aacatcactg taaagccgta attcaacgcc agcataaagg ccagcggtgg
     5941 accgattacc catgccagag aaagctgcgc acgcatcacc gagctaaaca tcaccacttc
     6001 tcgcgccgag ttatccgcgt attcccgcgc cagagcaaat aactgtggca ttgccgtatt
     6061 ggccagagat gccagaagca caccacaggt gataagcgtc agataatgac gattaaatgc
     6121 aaacagtagc gcattgccaa tagccatcaa acagcaaaat ataatcagtt ttcgccgatc
     6181 gccctgactg tcagaacgtt ttgccaacca gaggcttacg ccgatcccag caatagcatt
     6241 caccgtataa aagagtccga tccagaaagg ttgcgcgcca acctcacgac tcagaaataa
     6301 gctcaatgta ggagcctgta gcgccccggc cacccccatc ataaaagcga ccagcataaa
     6361 tgccgcgtaa acaccgttca tacggcgagc catcgtcatt atccagatca tacgttccct
     6421 ttttagcgcg gcgagaataa aaaaaaccag caggtataat ctgctggcgg gtgattttac
     6481 accaatactc agtcacacat gatgcaggca agtcaacttt cagaattgcg gtcatcccac
     6541 tgcatcagtc cttcaagcat ggttaatcgt tgtgggacgc ttaaccaacg caaccagcac
     6601 aacttcgctg tcgcggtaaa atagtgctga taaaactgac gcatggggca cctccttgct
     6661 tcatcgttag gaattattga cttaatatag ggaaaataaa attgctgtct tttgcgcagg
     6721 agttcccctt ttatgccatc tgctcgtctg caacaacagt tcatccgcct gtggcaatgc
     6781 tgcgagggta aatcgcagga cacaacgctc aacgaactgg cagcgttatt gagctgctcg
     6841 cgtcgtcata tgcgtaccct gctcaacacc atgcaggatc gcggctggct gacgtgggaa
     6901 gcggaagtcg ggcgcggtaa acgctcgcgt ctgacattcc tctataccgg gctggcgctt
     6961 cagcaacagc gggcggaaga cctgctggag caggatcgta tcgaccaact agtgcagttg
     7021 gttggcgaca aagcgactgt gcggcaaatg ctggtttctc atctgggccg cagcttccgc
     7081 caggggcggc acatcctgcg cgtgctctac tatcgtccgt tgcgtaatct gctacctggc
     7141 agcgcattgc gccgttccga aacccatatt gcccggcaaa tcttcagttc gctaacgcgc
     7201 ataaatgagg aaaatgggga actggaagca gacatcgccc accactggca gcaaatatca
     7261 ccgcttcact ggcgtttctt tttgcgtcca ggagtccatt ttcatcatgg tcgtgaactg
     7321 gaaatggacg acgtgatcgc ctctttaaaa cgaatcaata cgctgccgct ctattcgcat
     7381 attacggaca ttgtgtcgcc gacgccctgg acgctggata tccatctcac gcagccggac
     7441 cgctggttgc cgttactgct ggggcaagtt ccggcgatga tcctgccgtg cgaatgggaa
     7501 accctcagta actttgccag ccatcctatt ggcaccggtc cgtatgcggt gattcgcaac
     7561 agcaccaatc aactgaaaat tcaggcattc gatgacttct tcggttaccg ggcattaatc
     7621 gacgaagtta acgtctgggt tctgccggaa attgccgacg agccagccgg tggtctgatg
     7681 ctaaaaggtc cacagggcga gaaaaaagag attgaaagcc gcctggagga aggttgctac
     7741 tatttactgt tcgacagccg cacccatcgc ggggcgaatc agcaagtcag ggactgggta
     7801 agctatgtgc tttctccaac taatctggtc tatttcgctg aggaacagta ccagcaactg
     7861 tggttcccgg cttatggact gctcccccgt tggcaccatg cccgcaccat aaagagcgaa
     7921 aaaccggctg gcctggaaag cctcaccctg accttttatc aggatcacag tgagcatcgg
     7981 gtgattgccg ggatcatgca gcagattctg gcaagtcacc aggtcacgct ggaaatcaaa
     8041 gagatcagct acgatcagtg gcatgaagga gagatcgaga gcgatatctg gcttaatagc
     8101 gccaacttta cgctgccgct ggatttttcg ctgttcgcgc acttgtgcga ggtgccgctg
     8161 ctccaacact gtattccgat cgactggcaa gccgacgccg cccgctggcg caatggcgaa
     8221 atgaacctgg cgaactggtg ccagcaactg gtcgccagta aagcaatggt gccgcttatc
     8281 caccactggc tgatcattca gggacaacgc agtatgcgcg gcctgcgtat gaataccctc
     8341 ggctggttcg attttaaatc agcgtggttt gcgccaccgg atccatgatt gctggtagat
     8401 aacaaaatca ctacactaac gccgttctca acggggtgcc acgcaaacgc gtgcgctgag
     8461 aaaatacccg tcgaacctga tccggataac gccggcgaag ggatttgagg ctccttctca
     8521 agtcctttgc cactcttttt tgaggtgcaa agtgttaaaa aaatgtcttc ccctgctgtt
     8581 gctgtgcaca gcgcccgttt tcgctaaacc cgttctgact gtttatacct acgattcctt
     8641 cgccgccgac tgggggcctg gtccaaagat caaaaaagct tttgaagcca actgtaactg
     8701 cgaactgaaa ctggtggcgc tggaagatgg cgtttcgctt ctcaaccgtc tacggatgga
     8761 aggcaaaaat agtaaagccg atgtggtgct ggggctggat aacaatctgt tagacgccgc
     8821 cagtaaaacc ggactatttg ccaaaagcgg tgtggcagcg gatgccgtta acgttcccgg
     8881 cggctggaat aatgacactt tcgtcccgtt tgattacggc tatttcgcct tcgtctatga
     8941 caagaacaaa ctgaaaaacc cgccgcaaag cctgaaagaa ttggttgaga gcgatcagaa
     9001 ctggcgggtg atttatcagg atccgcgtac cagcacgccg gggctgggtc tgctgttgtg
     9061 gatgcaaaaa gtctatggcg atgacgcccc acaagcctgg caaaaactgg cgaagaaaac
     9121 cgtcacggtc accaaaggct ggagcgaagc ctacggcctg tttttaaaag gtgaaagcga
     9181 tctggtactg agttacacca cctctccggc ttatcacatt ctcgaagaga aaaaagataa
     9241 ctacgccgcc gcgaacttta gcgaaggtca ctatctgcag gtggaagtcg ccgcccgcac
     9301 cgctgccagc aagcagccgg agctggcgca aaaattcctc cagtttatgg tttctccggc
     9361 ttttcagaat gcgatcccaa ccggtaactg gatgtatccg gtggcaaatg tcacgctgcc
     9421 tgccgggttt gaacaattga ccaaaccggc caccacgctg gagttcacac cagctgaagt
     9481 ggcggcacaa cgtcaggcat ggattagcga atggcaacgc gccgtcagcc gttaattccc
     9541 ggctggttaa ttccaggtgt aagcgccgcc acgctggtgg tagcggttgc gctcgcggcg
     9601 tttctcgccc tgtggtggaa cgcgccgcag ggtaactggg tggcagtctg gcaggacagc
     9661 tacctgtggc atgtggtgcg cttctccttc tggcaggcgt ttctctcggc gctactctct
     9721 gtcgtacccg cgatattcct cgcccgcgcc ctctatcgca ggcgctttcc gggtcggctg
     9781 gcgctgttgc gtctgtgcgc aatgaccttg atcctcccgg tgctagttgc tgttttcggc
     9841 attcttagcg tctatggtcg tcagggctgg ctggcatcgc tctgccaatc gctcggtctg
     9901 gagtggacct tttcgcccta cggcctgcaa ggcattttgc tggcgcacgt attttttaat
     9961 ctgccgatgg cgagccgctt attactccag gcactggaaa acattcccgg cgaacaacgt
    10021 cagcttgccg cccagcttgg gatgcgcggc tggcattttt tccgcttcgt cgaatggccg
    10081 tggctacggc gacaaatccc gccggttgcc gcgcttatct ttatgctctg tttcgccagc
    10141 ttcgccaccg tgctatcgct gggcggcggt ccgcaggcaa ccactatcga gctggctatt
    10201 tatcaggcgc tgagttacga ctacgatcct gcccgcgcgg cgatgctggc gctgctccag
    10261 atggtgtgtt gccttgggct ggtgctgttg agtcagcgac taagtaaggc cattgcgccc
    10321 ggcaccacgc tgctgcaagg ctggcgcgat ccggacgatc gtctgcatag ccgcatttgc
    10381 gacacggtgt taatagtgct ggcgctgctg ctgttgctgc caccgttgct ggcggtgatc
    10441 gtcgatgggg taaatcgcca gttgccggaa gtgctggcac aaccggtgct gtggcaggcg
    10501 ctgtggacct cgttgcgtat tgcgctggcg gcaggtgtat tgtgcgtagt gctgaccatg
    10561 atgctgctat ggagcagtcg cgaactgcgg gcgcggcaga aaatgctggc gggtcaggcg
    10621 ctggagatga gcggcatgtt gatcctcgcc atgccgggga ttgtgctggc taccggcttc
    10681 tttttactgc tcaacaacac tatcggcctg ccacaatctg ctgacggcat tgtgattttc
    10741 accaatgcgt taatggcgat cccttatgcg ctgaaagtac tggaaaaccc gatgcgcgat
    10801 atcaccgccc gctacagtat gttgtgtcag tcgctgggca ttgaaggctg gtcgcgctta
    10861 aaagtggtcg agctgcgcgc cctgaaacgt ccactggcgc aggcgctggc ttttgcctgc
    10921 gtgctgtcga ttggtgattt tggcgtagtg gcgttgttcg gtaacgatga tttccgtacc
    10981 ctgccgtttt atctctacca gcaaattggc tcctatcgca gccaggacgg cgcggtcacc
    11041 gcgttaattc tgctactact ctgttttctg ctgtttaccg tgattgaaaa actaccgggg
    11101 cgaaatgtta aaactgactg atatcacctg gctttatcac catttgccga tgcgttttag
    11161 cttaacggta gaacgcggcg agcaggtggc aatcctcggg ccaagcggcg cgggtaaaag
    11221 taccctgctg aatttgattg ccggttttct gacgccagcc agcggttcgc tgactatcga
    11281 tggtgttgat cacaccacga cgccgccgtc acgccgtccg gtgtcgatgc tgtttcagga
    11341 gaacaacctg ttcagccatc tgacggtcgc gcagaacatc gggctggggc taaatccggg
    11401 gttgaaactg aacgcggcac agcaggagaa aatgcacgcg attgcccgtc agatggggat
    11461 tgataattta atggcgcggt taccgggcga gctttccggc ggtcagcgac agcgcgtggc
    11521 gttagcgcgt tgtctggtgc gcgaacagcc gattttattg ctcgatgaac cgttctctgc
    11581 gctcgatccg gcgttacgtc aggagatgct gacgctggtg agcacgagct gccagcagca
    11641 aaaaatgacg ctattgatgg tgtcgcacag tgtggaagat gcggcgcgga tcgccacacg
    11701 ctcggtggtg gtcgccgacg ggcgcatcgc ctggcagggt aagactgaag aattgttgag
    11761 cggtaaggcg agtgcttcgg caatattggg gattacgggt tagtatgcac tgtcagatgc
    11821 ggcgtgaaca ccttatccga cctacggaca gtgcgttttg taggcctgat aagacgcggt
    11881 cagcgtcgca tgaggcaacc cgcacaagac aatcccctaa accccaacca ctttacgcaa
    11941 aatatcgata tacaccggca tcaacgggtg gcgaattaac accaccagcg ccaccacgcc
    12001 gatggcagaa atcaacggcg tcagccacaa caaacggccg cgcgacaaat aatgacttaa
    12061 acgatcggtt gctttaccgc tgcgccataa ccgccagcac agccagccac caacccacaa
    12121 aaataccgcc gtcgccagca gcaaccattt aaactcgccg ctctgcattc cggctggaat
    12181 atcgatcgcc gcgcccgcca gaatcccagg caggaagtaa aacggtggcc acagcaggca
    12241 gccgataata ttcggcgtaa tgaattttgc caccggcaga tccagcattc ccgccaccat
    12301 tggcaccagc ggacgcgtcg ggccaacaaa gcgaccgacc agaatggtga acatgctgtg
    12361 ttgatgcaac gcatgttcag ttttatcgag taatgctttg tttttcttca gaaatgacca
    12421 gcgatgcaac ggctttttaa aacgccagcc cagccagaag gagatccagt cgcccagcag
    12481 acaaccgaca atccctgcca gccaagcgtg ccagaaactt aactcgccgc tgccaatcag
    12541 cgctcccagc cccgccatca gcaccgtacc gggtagaatc aaaccgacca gcgccagcga
    12601 ctccagaaag gccaccaaca ccaccgccat caatgaatac acggtagatt gggtaataaa
    12661 gtgttccagc aatgcttgca taatgtgcct gtcaaatgga cgaagcaggg attctgcaaa
    12721 ccctatgcta ctccgtcaag ccgtcaattg tctgattcgt taccaattat gacaacttga
    12781 cggctacatc attcactttt tcttcacaac cggcacggaa ctcgctcggg ctggccccgg
    12841 tgcatttttt aaatacccgc gagaaataga gttgatcgtc aaaaccaaca ttgcgaccga
    12901 cggtggcgat aggcatccgg gtggtgctca aaagcagctt cgcctggctg atacgttggt
    12961 cctcgcgcca gcttaagacg ctaatcccta actgctggcg gaaaagatgt gacagacgcg
    13021 acggcgacaa gcaaacatgc tgtgcgacgc tggcgatatc aaaattgctg tctgccaggt
    13081 gatcgctgat gtactgacaa gcctcgcgta cacgattatc catcggtgga tggagcgact
    13141 cgttaatcgc ttccatgcgc cgcagtaaca attgctcaag cagattcatc gccagcagct
    13201 ccgaatagcg cccttcccct tgcccggcgt taatgatttg cccaaacagg tcgctgaaat
    13261 acggctggtg cgcttcatcc gggcgaaaga aacccgtatt ggcaaatatt gacggccagt
    13321 taagccattc atgccagtag gcgcgcggac gaaagtaaac ccactggtga taccattcgc
    13381 gagcctccgg atgacaaccg tagtgatgaa tctctcctgg cgggaacagc aaaatatcac
    13441 ccggtcggca gacaaattct cgtccctgat ttttcaccac cccctgaccg cgaatggtga
    13501 gattgagaat ataacctttc attcccagcg gtcggtcgat aaaaaaatcg agataaccgt
    13561 tggcctcaat cggcgttaaa cccgccacca gatgggcatt aaacgagtat cccggcagca
    13621 ggggatcatt ttgcgcttca gccatacttt tcatactccc gccattcaga gaagaaacca
    13681 attgtccata ttgcatcaga cattgccgtc actgcgtctt ttactggctc ttctcgctaa
    13741 cccaaccggt aaccccgctt attaaaagca ttctgtaaca aagcaggacc aaagccatga
    13801 caaaaacgcg taacaaaagt gtctataatc acggcagaaa agtccacatt gattatttgc
    13861 acggcgtcac actttgctat gccatagcat ttttatccat aagattagcg gatcctacct
    13921 gacgcttttt atcgcaactc tctactgttt ctccataccc gtttttttgg atggagtgaa
    13981 acgatggcga ttgcaattgg cctcgatttt ggcagtgatt ctgtgcgagc tttggcggtg
    14041 gactgcgcca gcggtgaaga gatcgccacc agcgtagagt ggtatccccg ttggcaaaaa
    14101 gggcaatttt gtgatgcccc gaataaccag ttccgtcatc atccgcgtga ctacattgag
    14161 tcaatggaag cggcactgaa aaccgtgctt gcagagctta gcgtcgaaca gcgcgcagct
    14221 gtggtcggga ttggcgttga cagtaccggc tcgacgcccg caccgattga tgccgacggt
    14281 aacgtgctgg cgctgcgccc ggagtttgcc gaaaacccga acgcgatgtt cgtattgtgg
    14341 aaagaccaca ctgcggttga agaagcggaa gagattaccc gtttgtgcca cgcgccgggc
    14401 aacgttgact actcccgcta tattggcggt atttattcca gcgaatggtt ctgggcaaaa
    14461 atcctgcatg tgactcgcca ggacagcgcc gtggcgcaat ctgccgcatc gtggattgag
    14521 ctgtgcgact gggtgccagc tctgctttcc ggtaccaccc gcccgcagga tattcgtcgc
    14581 ggacgttgca gcgccgggca taaatctctg tggcacgaaa gctggggcgg cttgccgcca
    14641 gccagtttct ttgatgagct ggacccgatc ctcaatcgcc atttgccttc cccgctgttc
    14701 actgacacct ggactgccga tattccggtg ggcaccttat gcccggaatg ggcgcagcgt
    14761 ctcggcctgc ctgaaagcgt ggtgatttcc ggcggcgcgt ttgactgcca tatgggcgca
    14821 gttggcgcag gcgcacagcc taacgcactg gtaaaagtta tcggtacttc cacctgcgac
    14881 attctgattg ccgacaaaca gagcgttggc gagcgggcag ttaaaggtat ttgcggtcag
    14941 gttgatggca gcgtggtgcc tggatttatc ggtctggaag caggccaatc ggcgtttggt
    15001 gatatctacg cctggtttgg tcgcgtactc ggctggccgc tggaacagct tgccgcccag
    15061 catccggaac tgaaagcgca aatcaacgcc agccagaaac aactgcttcc ggcgctgacc
    15121 gaagcatggg ccaaaaatcc gtctctggat cacctgccgg tggtgctcga ctggtttaac
    15181 ggtcgtcgca cgccaaacgc taaccaacgc ctgaaagggg tgattaccga tcttaacctc
    15241 gctaccgaca ctccgctgct gttcggcggt ttgattgctg ccaccgcctt tggcgcacgc
    15301 gcaatcatgg agtgctttac cgatcagggg atcgccgtca ataacgtgat ggcgctgggc
    15361 ggcatcgcgc ggaaaaacca ggtcattatg caggcctgct gcgacgtgct gaatcgcccg
    15421 ctgcaaattg ttgcctctga ccagtgctgt gcgctcggtg cggcgatttt tgctgccgtc
    15481 gccgcgaaag tgcacgcaga catcccatca gcccagcaaa aaatggccag tgcggtagag
    15541 aaaaccctgc aaccgcgcag cgaacaggca caacgctttg aacagcttta tcgccgctat
    15601 cagcaatggg cgatgagcgc cgaacaacac tatcttccaa cttccgcccc ggcacaggct
    15661 gcccaggccg ttgcgactct ataaggacac gataatggcg atttttgata attatgaagt
    15721 gtggtttgtc attggcagcc agcatctgta tggcccggaa accctgcgtc aggtcaccca
    15781 acatgccgag cacgttgtta atgcgctgaa tacggaagcg aaactgccct acaaactggt
    15841 gttgaaaccg ctgggcacca cgccggatga aatcaccgct atttgccgcg acgcgaatta
    15901 cgacgatcgt tgcgctggtc tggtggtgtg gctgcacacc ttctccccgg ccaaaatgtg
    15961 gatcaacggc ctgaccatgc tcaacaaacc gttgctgcaa ttccacaccc agttcaacgc
    16021 ggcgctgccg tgggacagta tcgatatgga ctttatgaac ctgaaccaga ctgcacatgg
    16081 cggtcgcgag ttcggcttca ttggcgcgcg tatgcgtcag caacatgccg tcgttaccgg
    16141 tcactggcag gataaacaag cccatgagcg tatcggctcc tggatgcgtc aggcggtctc
    16201 taaacaggat acccgtcatc tgaaagtctg ccgatttggc gataacatgc gtgaagtggc
    16261 ggtcaccgat ggcgataaag ttgccgcaca gatcaagttc ggtttctccg tcaatacctg
    16321 ggcggttggc tatctggtgc aggtggtgaa ctccatcagc gacggcgatg ttaacgcgct
    16381 ggtcgatgag tacgaaagct gctacaccat gacgcctgcc acacaaatcc acggcgaaaa
    16441 acgacagaac gtgctggaag cggcgcgtat tgagctgggg atgaagcgtt tcctggaaca
    16501 aggtggcttc cacgcgttca ccaccacctt tgaagatttg cacggtctga aacagcttcc
    16561 aggtctgccc gtacagcgtc tgatgcagca gggttacggc tttgcgggcg aaggcgactg
    16621 gaaaaccgcc gccctgcttc gcatcatgaa ggtgatgtca accggtctgc agggcggcac
    16681 ctcctttatg gaggactaca cctatcactt cgagaaaggt aatgacctgg tgctcggctc
    16741 ccatatgctg gaagtctgcc cgtcgattgc cgtagaagag aaaccgatcc tcgacgttca
    16801 gcatctcggt attggtggta aggacgatcc tgcccgactg atcttcaata cccaaaccgg
    16861 tccagcgatt gtcgccagcc tgattgatct cggcgatcgt taccgtctgc tggttaactg
    16921 tatcgacacg gtgaaaacac cgcactccct gccgaaactg ccggtggcga atgcactgtg
    16981 gaaagcgcaa ccggatctgc caactgcttc cgaagcgtgg atcctcgctg gtggcgcgca
    17041 ccataccgtc ttcagccatg cgctgaacct caacgatatg cgccagttcg ccgagatgca
    17101 cgacattgaa atcacggtga ttgataacga cacccgcctg ccagcgttta aagacgcact
    17161 gcgctggaac gaagtgtatt acggatttcg tcgctaagta gccacatcag gtatgtaacg
    17221 cctgatgcga cgctgacgcg tcttatctgg cctacacgct gcgattttgt aggccggata
    17281 agcaaagcgc atccggcatt caacgcctga tgcgacgctg gcgcgtctta tcaggcctac
    17341 gcgctgcgat tttgtagacc ggataagcga agcgcatccg gcacgaagga gtcaacatgt
    17401 taaaagatct caaacgccag gtattagaag ccaacctggc gctgccaaaa cacaacctgg
    17461 tcacgctcac atggggcaac gtcagcgccg ttgatcgcga gcgcggcgtc tttgtgatca
    17521 aaccttccgg cgtcgattac agcgtcatga ccgctgacga tatggtcgtg gttagcatcg
    17581 aaaccggtga agtggttgaa ggtacgaaaa agccctcctc cgacacgcca actcaccggc
    17641 tgctctatca ggcattcccc tccattggcg gcattgtgca tacgcactcg cgccacgcca
    17701 ccatctgggc gcaggcgggt cagtcgattc cagcaaccgg caccacccac gccgactatt
    17761 tctacggcac cattccctgc acccgcaaaa tgaccgacgc agaaatcaac ggcgaatatg
    17821 agtgggaaac cggtaacgtc atcgtagaaa cctttgaaaa acagggtatc gatgcagcgc
    17881 aaatgcccgg cgttctggtc cattcccacg gcccgtttgc atggggcaaa aatgccgaag
    17941 atgcggtgca taacgccatc gtgctggaag aggtcgctta tatggggata ttctgccgtc
    18001 agttagcgcc gcagttaccg gatatgcagc aaacgctgct gcataaacac tatctgcgta
    18061 agcatggcgc gaaggcatat tacgggcagt aatgactgta taaaaccaca gccaatcaaa
    18121 cgaaaccagg ctatactcaa gcctggtttt ttgatggatt ttcagcgtgg cgcaggcagg
    18181 ttttatctta acccgacact ggcgggacac cccgcaaggg acagaagtct ccttctggct
    18241 ggcgacggac aacgggccgt tgcaggttac gcttgcaccg caagagtccg tggcgtttat
    18301 tcacgccgat caggttcccc gcgctcagca tattttgcag ggtgaacaag gctttcgtct
    18361 gacaccgctg gcgttaaagg attttcaccg ccagccggtg tatggccttt actgtcgcgc
    18421 ccatcgccaa ttgatgaatt acgaaaagcg tctgcgtgaa ggtggcgtta ccgtctacga
    18481 ggccgatgtg cgcccgccag aacgctatct gatggagcgg tttatcacct caccggtgtg
    18541 ggtcgagggt gatatgcaca atggcactat cgttaatgcc cgtctgaaac cgcatcccga
    18601 ctatcgtccg ccgctcaagt gggtttctat agatattgaa accacccgcc acggtgagct
    18661 gtactgcatc ggcctggaag gctgcgggca gcgcatcgtt tatatgctgg ggccggagaa
    18721 tggcgacgcc tcctcgcttg atttcgaact ggaatacgtc gccagccgcc cgctgctgct
    18781 ggaaaaactc aacgcctggt ttgcaaacca cgatcccgat gtgatcatcg gctggaacgt
    18841 ggtgcagttc gatctgcgaa tgctgcaaaa acatgccgag cgttaccgtc ttccgctgcg
    18901 tcttgggcgc gataatagcg agctggagtg gcgcgagcac ggctttaaaa acggcgtctt
    18961 ttttgcccag gctaaaggtc ggctaattat cgacggtatc gaggcgctga aatccgcgtt
    19021 ctggaatttc tcttcattct cgctggaaac tgtcgctcag gagctattag gcgaaggaaa
    19081 atctatcgat aacccgtggg atcgaatgga cgaaattgac cgccgtttcg ccgaagataa
    19141 acctgcgctg gcaacttata acctgaaaga ttgcgagctg gtgacgcaga tcttccacaa
    19201 aactgaaatc atgccatttt tactcgaacg agcaacggtg aacggcctgc cggtggatcg
    19261 gcacggcggt tcggtggcag cgtttggtca tctctatttt ccgcgaatgc atcgcgctgg
    19321 ttatgtcgcg cctaatctcg gcgaagtgcc gccgcacgcc agccctggcg gctacgtgat
    19381 ggattcacgg ccagggcttt atgattcagt gctggtgctg gactataaaa gcctgtaccc
    19441 gtcgatcatc cgcacctttc tgattgatcc cgtcgggctg gttgaaggca tggcgcaacc
    19501 tgatccagag cacagtaccg aaggttttct cgatgcctgg ttctcacggg agaaacattg
    19561 cctgccggag attgtgacta acatctggca cgggcgcgat aaagccaaac gtcagggtaa
    19621 taagccgctt tcgcaggcgc tgaagatcat tatgaatgcc ttttatggcg tgctcggcac
    19681 caccgcctgc cgcttcttcg atccgcgcct ggcatcgtcg atcaccatgc gtggtcatca
    19741 gatcatgcgg cagaccaaag cattgattga agcgcagggc tacgacatta tctacggaga
    19801 taccgactcg acttttgtct ggctaaaagg tgcacattcg gaagaagagg cggcgaaaat
    19861 cggtcgtgca ctggtgcagc acgttaacgc ctggtgggcg gaaacgctgc aaaaacaacg
    19921 gctgaccagc gcattagaac tggagtatga aactcatttc tgccgttttc ttatgccaac
    19981 cattcgcgga gctgataccg gcagtaaaaa gcgctacgcc gggctgattc aggagggcga
    20041 caagcagcgg atggtgttta aagggctgga aaccgtgcgc accgactgga cgccgctggc
    20101 ccagcagttt cagcaggagc tgtacctgcg tatcttccgc aacgagccat atcaggagta
    20161 cgtccgccaa accatcgaca aactgatggc gggtgaactg gatgcgcggc tggtttaccg
    20221 taaacgcctt cgccgtccgc tgagcgaata tcagcgtaat gttccgcctc atgtacgcgc
    20281 cgctcgcctt gccgatgaag aaaatcaaaa gcgcggtcgc cccttgcaat atcagaatcg
    20341 cggcaccatt aagtacgtat ggaccaccaa cggcccggag ccgctggact accaacgttc
    20401 accactggat tacgaacact atctgacccg ccagctacaa cccgtggcgg agggaatact
    20461 cccttttatt gaggataatt ttgctacact tatgaccggg caacttgggc tattttgagc
    20521 aaaaaaaaga gttcgccaga taccattttg atgcgtgacg aatgctttgc catccagtac
    20581 catagcgccc tttccattcc tggacctgaa taacaccact acctcataag cacggtagcg
    20641 ggtggttatt gcctgcaatt aaagatatag agccgaacac atatgccttt tacacttggt
    20701 caacgctgga tcagcgatac agaaagcgaa ttgggacttg gaaccgttgt cgcggtggat
    20761 gcgcgaactg tcactttact tttcccatct actggtgaaa accgtctgta cgcacgcagt
    20821 gattcccccg tgacccgcgt gatgttcaac cctggtgata ccattaccag ccatgacggc
    20881 tggcagatgc aagtcgaaga agtaaaagaa gaaaatggct tgctgaccta tatcggtact
    20941 cgcctggata ctgaagagtc cggcgtagcc ctgcgtgaag ttttccttga tagcaaactg
    21001 gtgttcagca aaccgcagga ccgtctgttt gccgggcaga ttgaccgtat ggaccgcttt
    21061 gcgctgcgtt atcgcgcgcg taagtattcc agcgaacagt tccgtatgcc gtacagcggc
    21121 ctgcgcggtc agcgtaccag cctgatcccg catcagctca acatcgctca tgatgttggt
    21181 cgccgccacg cgccgcgcgt cctgctggct gacgaagtgg gtttagggaa aaccattgaa
    21241 gccgggatga tcctgcatca gcaactgctc tctggcgctg ctgaacgtgt gctgattatc
    21301 gtcccggaaa ccttacagca tcagtggctg gtagaaatgc tgcgccgttt caacctgcgc
    21361 tttgctctgt ttgatgatga gcgttatgcc gaagctcagc acgatgctta caacccgttt
    21421 gacaccgaac agctggtgat ttgctcgctg gattttgccc gtcgtagcaa acagcgcttg
    21481 gaacatctct gtgaagccga atgggacctg ctggtggtcg atgaagcgca tcacctggtg
    21541 tggagcgaag atgcgccaag ccgtgaatat caggccattg aacaactggc agagcacgtg
    21601 ccgggcgttc tgctgctgac cgcgaccccg gaacagctgg ggatggaaag ccacttcgcc
    21661 cgtctgcgtc tgctggaccc gaaccgtttc cacgattttg cgcagttcgt tgaagagcag
    21721 aaaaattatc gtccggttgc ggacgccgtt gccatgctgc tggcaggtaa caaactgagc
    21781 aatgacgaac tgaacatgct cggcgagatg atcggcgagc aggatatcga gccgctgttg
    21841 caggcagcaa acagcgacag cgaagatgcc cagagcgccc gtcaggagct ggtttcgatg
    21901 ctgatggatc gccacggcac cagccgcgtg ctgttccgta acacgcgtaa cggtgtgaaa
    21961 ggattcccga aacgcgagct gcacaccatt aagctgccgc taccgacgca gtatcagacg
    22021 gctattaaag tctccggcat tatgggcgca cgtaaaagtg cggaagatcg tgctcgcgat
    22081 atgctctacc cggagcgtat ttatcaggaa tttgaaggtg ataacgccac ctggtggaac
    22141 ttcgatccgc gcgttgagtg gctgatgggc tacctgacca gccatcgctc tcagaaagtg
    22201 ctggtgatct gcgccaaagc tgccactgcg ctgcaactgg agcaggtact gcgcgaacgt
    22261 gaaggtattc gcgctgcggt gttccacgaa ggtatgtcga ttatcgaacg tgaccgcgct
    22321 gccgcctggt ttgccgaaga agacaccggc gcacaggtac tgctgtgctc agaaatcggt
    22381 tctgaaggac gtaactttca gttcgccagt cacatggtga tgtttgacct gccattcaac
    22441 ccggatctac tggagcagcg tattggtcgt ctggatcgta tcggccaggc gcacgatatt
    22501 cagatccatg tgccttatct ggagaaaacc gctcagtcgg tgctggtgcg ctggtatcac
    22561 gaaggtctgg atgcatttga gcacacctgc ccgaccggac gcactattta cgatagcgta
    22621 tacaacgatc tgattaacta tctggcttca ccggatcaaa ccgaaggctt tgacgatctg
    22681 atcaaaagct gccgcgagca acatgaagcg ctgaaagcac agctggaaca gggtcgtgac
    22741 cgcctgctgg aaatccactc caacggtggc gaaaaagccc aggcactggc agaaagcatt
    22801 gaagagcagg atgacgatac caacctgatc gccttcgcca tgaacctgtt cgatattatc
    22861 ggtatcaatc aggacgatcg cggcgacaac atgatcgtgc tgacgccgtc cgatcatatg
    22921 ctggtgccgg acttccctgg cctgtcggaa gatggcatca ccatcacctt tgatcgtgaa
    22981 gtggcgctgg cgcgtgaaga tgcacagttt attacctggg agcatccgct gatccgcaac
    23041 ggtctggatc tgatcctttc tggcgatacc ggtagcagca cgatttcact gttaaaaaac
    23101 aaagcgttgc cggtaggtac gctgttggtg gaactgattt atgtggttga agcccaggct
    23161 ccgaagcagt tgcagctcaa ccgcttcctg ccaccgacgc cggtacgtat gctgctggat
    23221 aaaaacggca acaacctggc ggcgcaggta gagtttgaaa cctttaaccg ccagcttaac
    23281 gcggttaacc gtcacaccgg cagcaaactg gttaacgccg tgcagcagga tgttcacgct
    23341 atccttcaac tgggtgaagc gcagatcgag aaatctgccc gtgcattgat tgatgcagcg
    23401 cgtaacgaag ccgacgaaaa actgtctgcc gagctgtctc gtctggaagc tctgcgtgca
    23461 gtgaacccga acattcgtga cgacgaactg accgccattg agagcaaccg tcagcaggta
    23521 atggaaagcc tggatcaggc aggttggcgt ctggatgccc tgcgtttgat cgttgtaacg
    23581 catcagtaac ggagccgaaa atggggatgg aaaactacaa tccaccgcag gaaccctggt
    23641 tggttatcct gtatcaggat gaccatatta tggtggtcaa caagccgagc ggtttgttgt
    23701 cagtgccggg tcgtctggaa gagcacaaag acagcgtgat gacgcgcatt cagcgtgatt
    23761 atccgcaggc agaatcggtg catcgtctgg atatggctac cagcggcgtg attgtagtgg
    23821 cgctgaccaa agccgcggag cgggagttaa aacgccagtt ccgcgagcgc gagccgaaaa
    23881 agcagtatgt ggcccgcgtc tgggggcatc catcccccgc agaaggtctg gtggatctgc
    23941 cgctgatttg cgactggcca aaccgcccga aacagaaagt ctgttacgaa acgggtaaac
    24001 ctgcgcagac ggaatatgaa gtggtggagt atgcggcgga taacacggca agagtggtgt
    24061 taaaaccgat taccgggcgt tcgcatcaat tgcgtgtgca tatgctggcg ctggggcatc
    24121 cgattctcgg cgatcgtttt tatgcatcac cagaagcgag agcgatggca ccgcgtttgt
    24181 tgctacatgc agagatgttg acgattaccc atccggcgta tggcaatagt atgacgttta
    24241 aagcgccagc ggatttttaa tcgtcacgct ggcgcagttc ggtaggcgga taaggcgttc
    24301 acgccgcatc cggcaattgt gcgccgattg cctgatgcga cgcgggccgc gtcttatcag
    24361 gcctacagtt taaaacttat ttaaaccctt tctgctgctt tatcagctca tatgcctgct
    24421 gaatttcctg cgctttctgc ttcgccatct ccatcatctc aggcggcaaa cctttcgcca
    24481 ccagcttatc gggatggtgt tcactcatca gcttacggta ggcacgtttg atggtggtcg
    24541 catcatccgt cggcttcacg cccagcacat tacaggcatc ttccagcgtc gggccacgct
    24601 gcgcttgctg ccagttacca ccgccagatt gctgctgata accgccgcca aactgtgcac
    24661 cgccctgcat catgcgcaaa aactggtcaa actgggcgcg ggagatccct aattcttcag
    24721 caatgacata cagcaccgcc cgttcattcg ggtgcagtga accatcagca aacgccgcct
    24781 gaatctggat ctccagaaac atacgaatta agtcaaaacg accaaagcag acactgcgaa
    24841 actggcgcat cttttcgcgc agcgggtaat tatctgattt tcccacccgg aacgcgtttt
    24901 gcgccgcagt acgggaagcg ccatgaaggt tcattcggtc catcaactgg ctggcgatat
    24961 gaatatcagc ctccgtgacg cgacctttgg atttggttaa atgccccatc acttcaaaag
    25021 tggtggcaaa aaacagcgcc tgacgctcac gctggttggc gaaccacgcc attttacggc
    25081 tacgggcttt atcaaacata tggccaatta acaggcctaa cactacgccc caaaagccgc
    25141 cgcccatcag taaggccacg gccacgccaa tgatttttcc ccaatactgc atatattccc
    25201 caaatcgaca cacggatatc agggctatct cccacaatat aaaggtgctt ttaccgtttt
    25261 ccggctgcgg tcaattgtgg gacatcgcct ataatttgca ttatcatacc tgtcattcaa
    25321 tgccgtgcct aacaccactg acgctattcg gacaggatta acactagcgt agagatgacg
    25381 agtacgttag tctctgaccg tttgtcacgc gcaacgttac cgatgatgga acaataaaat
    25441 caacgtatga aaaaacgtat ccccactctc ctggccacca tgattgccac cgccctttat
    25501 agtcaacagg gactggcagc cgacctcgcc tcacagtgca tgttgggcgt gccaagctat
    25561 gaccgtcctc tggtacaggg cgataccaat gacttacccg tgactatcaa tgctgaccac
    25621 gcgaaagggg actacccgga tgacgccgtg tttactggca gcgtggatat catgcagggt
    25681 aacagccgtc tgcaggccga cgaagtgcag ctccatcaaa aagaggcacc aggacaaccg
    25741 gagccggtac gtaccgttga tgcgctcggt aatgtccatt acgacgataa ccaggtgatc
    25801 ctcaaagggc cgaaaggctg ggcgaatctg aacaccaaag ataccaacgt ctgggaaggt
    25861 gattaccaga tggtgggtcg ccagggtcgc ggtaaagcgg acctgatgaa acaacgtggc
    25921 gaaaaccgct ataccattct ggataacggt agctttacct cctgtctgcc gggttctgac
    25981 acctggagcg tggtaggtag cgaaattatt catgaccgcg aagaacaagt tgctgagatc
    26041 tggaacgccc gctttaaggt gggtccggta ccgatctttt atagccccta tttgcagttg
    26101 ccggtgggtg acaaacgtcg ctctggtttc ttgatcccga acgccaagta caccaccacc
    26161 aactactttg agttctacct gccatattac tggaacatcg cgccaaatat ggatgccacc
    26221 atcacgccgc attatatgca tcgtcgtggc aacatcatgt gggagaacga attccgctac
    26281 ctctcccagg cgggcgctgg cttgatggaa ctggactatc tgccttcaga taaagtctat
    26341 gaagatgaac acccgaacga tgacagttca cgtcgttggt tattctactg gaaccactcc
    26401 ggggtcatgg atcaggtgtg gcgtttcaac gtcgactaca ccaaggtcag cgatcctagc
    26461 tacttcaatg atttcgataa caagtacggt tccagtactg acggctacgc aacgcaaaaa
    26521 ttcagcgttg gctatgcggt gcaaaacttc aatgccaccg tttcaaccaa gcagttccag
    26581 gttttcagcg aacagaacac cagtagctac tcggcagagc cgcagttaga cgttaattac
    26641 taccagaatg atgttggtcc gtttgatacg cgtatttacg gccaggcagt gcactttgtt
    26701 aacaccagag acgacatgcc tgaagcaacc cgtgttcacc tggaaccgac catcaatttg
    26761 ccgctctcta ataactgggg cagcatcaat accgaagcga agtttctggc aacccattat
    26821 cagcaaacca atcttgactg gtataactcc agaaacacga ccaagctgga cgaatccgtt
    26881 aaccgcgtaa tgccgcaatt caaagttgac ggcaaaatgg tctttgaacg cgatatggaa
    26941 atgctggctc cgggttatac ccaaacgctg gaaccgcgcg cgcagtattt gtacgtgccg
    27001 tatcgcgatc agagcgacat ctataactac gactcgtctc tgctgcaatc tgactactct
    27061 ggcctgttcc gggaccggac ttacggcggt cttgaccgta ttgcctccgc taaccaagtg
    27121 acgaccggtg tcacatctcg catatatgat gatgctgccg ttgaacgttt taatatttcc
    27181 gttggtcaaa tctactattt cacggagtct cgcactggcg atgacaacat aacatgggag
    27241 aatgacgaca aaacgggctc actggtgtgg gcaggcgaca cctactggcg tatctccgag
    27301 cgttggggat tgcgtggcgg gattcagtac gatacacgtc tggataacgt agcgaccagt
    27361 aactccagca ttgaataccg tcgggatgaa gaccgtctgg tacagctgaa ttaccattac
    27421 gccagcccgg aatatattca ggctacgctg cctaagtact attccactgc tgagcaatat
    27481 aagaatggta tttcgcaggt aggtgctgtc gccagcaggc caattgccga tcgttggtcc
    27541 attgttgggg cctactacta cgacaccaat gcgaacaagc aagccgactc tatgttaggt
    27601 gtgcaataca gctcctgctg ctatgcaatt cgcgtcggtt acgagcggaa gctgaacggt
    27661 tgggataacg ataaacaaca tgcggtatat gacaacgcaa tcggctttaa catcgaactt
    27721 cgcggcctga gctccaacta cggtctgggt acgcaagaga tgctgcgttc gaacattctg
    27781 ccgtatcaaa acactttgtg atttgttgat ttaccacgta atccgcagtg cggttaattg
    27841 aaatggaaaa agtatgaaga actggaaaac gctgcttctc ggtatcgcca tgatcgcgaa
    27901 taccagtttc gctgcccccc aggtagtcga taaagtcgca gccgtcgtca ataacggcgt
    27961 cgtgctggaa agcgacgttg atggattaat gcagtcggta aaactgaacg ctgcgcaggc
    28021 aaggcagcag cttcctgatg acgcgacgct gcgccaccaa atcatggaac gtttgatcat
    28081 ggatcaaatc atcctgcaga tggggcagaa aatgggagtg aaaatctccg atgagcagct
    28141 ggatcaggcg attgctaaca tcgcgaaaca gaacaacatg acgctggatc agatgcgcag
    28201 ccgtctggct tacgatggtc tgaactacaa cacttatcgt aaccagatcc gcaaagagat
    28261 gattatctct gaagtgcgta acaacgaggt gcgtcgtcgc atcaccatcc tgccacagga
    28321 agtcgaatcc ctggcgcagc aggtgggtaa ccaaaacgac gccagcactg agctgaacct
    28381 gagccacatc ctgatcccgc tgccggaaaa cccgacctct gatcaggtga acgaagcgga
    28441 aagccaggcg cgcgccattg tcgatcaggc gcgtaacggc gctgatttcg gtaagctggc
    28501 gattgctcat tctgccgacc agcaggcgct gaacggcggc cagatggggt ggggccgtat
    28561 tcaggagttg ccggggatct tcgcccaggc attaagcacc gcgaagaaag gcgacattgt
    28621 tggcccgatt cgttccggcg ttggcttcca tattctgaaa gttaacgacc tgcgcggcga
    28681 aagcaaaaat atctcggtga ccgaagttca tgctcgccat attctgctga aaccgtcgcc
    28741 gatcatgact gacgaacagg cccgcgtgaa actggagcaa attgctgctg atatcaagag
    28801 tggtaaaacg acttttgctg ccgcagcgaa agagttctcg caggatccag gttctgctaa
    28861 ccagggcggt gatctcggct gggctacacc agatattttc gatccagcct tccgtgacgc
    28921 cctgacccgc ctgaacaaag gtcaaatgag tgcaccggtt cactcttcat tcggctggca
    28981 tttaatcgaa ctgctggata cccgtaatgt cgataaaacc gacgctgcgc agaaagatcg
    29041 tgcataccgc atgctgatga accgtaagtt ctcggaagaa gcagcaagct ggatgcagga
    29101 acaacgtgcc agcgcctacg ttaaaatcct gagcaactaa tggttaaaac ccaacgtgtt
    29161 gtgatcactc ccggcgagcc cgccgggatt ggcccggact tagttgtcca gcttgcacag
    29221 cgtgagtggc cggtcgaact ggttgtttgt gccgatgcca ctctccttac cgaccgggca
    29281 gcgatgctcg gtttgccgct caccctccgc ccttattccc ccaactcccc tgcacaaccg
    29341 caaactacgg gcacattaac gctacttcct gtcgcgctac gtgaatctgt cactgcgggg
    29401 cagttagcga ttgaaaatgg gcattacgtg gtggagacgc tggcgcgagc gtgtgatggc
    29461 tgtctgaacg gtgaatttgc tgcgctgatc acaggccccg tgcataaagg cgtcattaac
    29521 gacgcaagca ttccgtttac cggtcatacc gagtttttcg aagagcgttc gcaggcgaaa
    29581 aaagtggtga tgatgctggc gaccgaagaa cttcgcgtgg cgctggcaac gacgcattta
    29641 ccgctgcgcg atatcgcaga tgctatcacc cctgcacttt tgcacgaagt gattgctatt
    29701 ttgcatcacg atttgcgtac aaaatttggt attgccgaac cgcgcattct ggtctgcggg
    29761 ctgaatccgc acgcgggcga aggcggtcat atgggtacgg aagagataga caccattatt
    29821 ccggtgctcg acgagctgcg ggcgcagggg atgaaactca acgggccgct gcctgccgat
    29881 accctgtttc agccgaaata tctcgataac gccgacgccg tgctggcgat gtaccacgat
    29941 cagggtcttc ccgtgctaaa ataccagggc ttcgggcgcg gtgtgaacat tacgctgggc
    30001 ctgcccttta ttcgcacatc agtggaccac ggcaccgcgc ttgaactggc gggacgtggc
    30061 gaagccgatg tcggcagttt tattacggcg cttaatctcg ccatcaaaat gattgttaac
    30121 acccaatgaa taatcgagtc caccagggcc acttagcccg taaacgcttc gggcaaaact
    30181 ttctcaacga tcagttcgtg atcgacagca ttgtttctgc cattaacccg caaaagggcc
    30241 aggcaatggt cgaaatcggc cccggtctgg cggcattgac cgagccggtc ggcgaacgtc
    30301 tggaccagct gacggtcatc gaacttgacc gcgatctggc ggcacgtctg caaacgcatc
    30361 cattcttagg cccgaaactg acgatttatc agcaggatgc gatgaccttt aactttggcg
    30421 aactggccga gaaaatgggt cagccgctgc gtgttttcgg caacctgcct tataacatct
    30481 ccacgccgtt gatgttccat ctgtttagct atactgatgc cattgccgac atgcacttta
    30541 tgttgcaaaa agaggtggtg aatcgtctgg ttgcaggacc gaacagcaaa gcgtatggtc
    30601 gattaagcgt catggcgcaa tactattgca acgtgatccc ggtactggaa gttccgccgt
    30661 cagcctttac accaccaccc aaagtggatt ccgccgtcgt gcgcctggtc cctcatgcaa
    30721 cgatgcctca cccggttaaa gatattcgcg tgttgagccg catcaccacc gaagccttta
    30781 accagcgtcg taaaaccatt cgtaacagcc tcggcaacct gtttagcgtc gaggtgttaa
    30841 ccggaatggg gatcgacccg gcgatgcgag cggaaaatat ctctgtcgcg caatattgcc
    30901 agatggcgaa ctatctggcg gagaacgcgc ctttgcagga gagttaacga tgatcaattc
    30961 gccccgagtg tgtattcagg ttcaaagcgt ctacattgag gcacaatctt cacctgataa
    31021 tgaacgttac gtttttgctt ataccgtaac catacgcaat ctggggcgag cgccagtgca
    31081 gttgttgggg cgttactggc tgatcaccaa tggcaatggc cgtgaaaccg aagtccaggg
    31141 cgaaggagtg gttggcgtcc agccacttat cgcgcctggc gaagagtacc agtacaccag
    31201 cggtgcaatc attgaaaccc cgctgggcac catgcagggt cactacgaaa tgatcgatga
    31261 aaatggcgtc cctttcagca tcgacattcc cgtattccga ctcgccgttc ccacactcat
    31321 tcattaaaag aatatggcga cataccttat tggcgacgtt catggttgtt acgatgaact
    31381 gatcgcattg ctgcataaag tagaatttac ccccgggaaa gataccctct ggctgacggg
    31441 cgatctggtc gcgcgcggcc cgggttcgct ggatgttctg cgctatgtga aatccttagg
    31501 cgacagcgta cgtctggtgc tgggcaatca cgatctgcat ctgctggcgg tatttgccgg
    31561 gatcagccgc aataaaccga aagatcgcct gacaccgctg ctggaagcgc cggatgccga
    31621 cgagctgctt aactggctgc gtcgccagcc tctgctgcaa atcgacgaag agaaaaagct
    31681 ggtgatggcc cacgcaggga tcacgccgca gtgggatctg cagaccgcca aagagtgcgc
    31741 gcgcgatgta gaagcggtgc tgtcgagtga ctcctatccc ttctttcttg acgctatgta
    31801 cggcgatatg ccaaataact ggtcaccgga attgcggggg ctgggaagac tgcgttttat
    31861 caccaacgcc tttacccgta tgcgtttttg cttcccgaac ggtcaactgg atatgtacag
    31921 caaagaatcg ccggaagaag cccctgcccc actgaaaccg tggtttgcga ttcctggccc
    31981 tgtcgctgaa gaatacagca tcgcctttgg tcactgggca tcgctggaag gcaaaggtac
    32041 gccggaaggt atttacgcgc tggataccgg ctgctgctgg ggtgggtcat taacctgcct
    32101 gcgctgggaa gataaacagt attttgtcca gccgtcgaac cggcataagg atttgggcga
    32161 agcggcggcg tcttaaacac agcctgatac aggaaggccg gataagacgc gaccggcgtc
    32221 gcatccggca ctagccgtaa attctataca aacttaccgc cgctccagaa tctcaaagca
    32281 atagctgtga gagttctgcg catcggcgtc gtggaattcg ctgaataccg attcccagtc
    32341 atccggctcg taatccggga aatgggtgtc gccttccact tctgcgtcga tatgcgtcag
    32401 atacagtttt tgcgcttttg gcaggaactg ctcataaacg cgaccgccgc caatcaccat
    32461 gatttctggt acgtcaccac acgccgcaat ggcttcatcc accgatttca cccacgttac
    32521 gcgatcgtcc gtacccggtt gactgctgag gataatattt ttgcgtcctg gcaacggacg
    32581 accgattgat tcccaagtat ggcggcccat aatcacgggt ttatttaagg tgttgcgttt
    32641 aaaccaggcg agatcggcag gcaggttcca cggcatggcg ttttccatgc cgataacgcg
    32701 atctaccgct aacgccgcaa tcagactgat cattgagatt tcccgataaa aaaattgtcg
    32761 ccactatacg taaagcgtaa accgtcgtcg actggtgcga ggatgatgtt gaggaaaatt
    32821 ttatattctg ctggcgagtc cacgctctcc ccctggactc gccgcattac aatgaaacaa
    32881 aaacaaacag ttagctgtaa agtgtgattt acgtcactct ttattaagat gagggtttcg
    32941 tttccggttc atccgccatg ttgccggtat gtttaccttc ttctgttccc tgccagccat
    33001 gtcgttgaat taatgacaga tgttcgcggt cctcggtgat gatctcactt aacatcgcgc
    33061 tggtgcgttt atagaccgcc gcgcgggctt tggtgtcatt ctcaaccatt gccatctctt
    33121 ccaccatctg aatattaaag cggcggaaca catcggcacg ttctcgcgct tcatacggcc
    33181 ccagacctaa actttccagc gccagacgcc cggttttcag cgcaccttca aaggtttcac
    33241 gctccggctt ttcaacgcct gcctgacgca aacgaatgta gtgatcgaca tcgcgggcgc
    33301 gggcaataat ctgcaaatgc gggaaatgtt ctttcaccat ctccgtcagt tgcaggttgg
    33361 tttgcggatc gtcaatagcg ttaatcagca cttccgcttt cgccgctccg gcagattcca
    33421 gtaaatccat ccgcgtggca tcgccataaa acactttcat accaaattta cgcagcgttt
    33481 cgatatggtc cggatcgtga tcgagcacca ccattttcac cccgctggag agcagtaaac
    33541 gtccggtaat ctgtccaaaa cgaccgaatc cggcgataat cacgcgcggc tgttcttcat
    33601 cgatctcatc ggcttcacgc gcctcctccg tagaagattg ctcaaggcga ttgaggatca
    33661 ccagcagaat cggcgttgct gccatcgaca gcgccaccgc cagggtcagc gatttcaccc
    33721 actccggctc cagcacattc gccatctgcg ccgcaccaaa taccacaaag gcaaactcac
    33781 tgccctgccc taacaacacc gcaaaccagc gacgctgttt atttggcacc tgcaacggtc
    33841 gggcaatcag ccacagcatg gcgattttga tgatgaggaa accgagcagc aaaatgacaa
    33901 tgcgcaatgg gttttcaatc agcgtgccaa agtctatcga catgccaaca ccgatgaaaa
    33961 acagccccaa cagcaaacct ttaaacggtt cgatatcgct ttccaacgca tgacggtatt
    34021 ccgagcttgc cagcagtacg cccgccagaa acgcgcccat cgccatcgac aagccgacct
    34081 cttccagcag caaaccaaag ccaaacacaa ggaataacgc cacggcacta aacacttccc
    34141 gcaagccaga gcgggcaaca aaacgcagcg ccggacgcgt aacatagcgc cccagcaata
    34201 ccaccagtac cagcgcaccc gccactttta acgccgagag agcaaatgcg cccatcgtcg
    34261 tcgaggcact gctcgccgcc agtagcggga tcatcgccac cagcgggatt gccgcgatat
    34321 cctggaacag cagcactgca aaggcactgc ggcccatttg cgtcaccatc agattgcgtt
    34381 cattcatcgc ctgcatggca atcgccgttg atgagagcgc cagcgtcata ccgatcaatt
    34441 ccgcgacctg ccagcgcagc ccaagtaaca tacagaacag ccccagcagg ccaccgcaaa
    34501 tcaccatctg caatgcgcca ccgccgaaca ctgccgcacg cagcttccac agcctttgtg
    34561 gatcgagttc gaggccgata ataaacagca tcagcaccac cccaatctcg gcaaagtgca
    34621 gaatagattc ggcatcggtc accagtcgca gcccccacgg gccaataatg cagccggcga
    34681 tcaggtagcc aagtaccgat cccagaccaa gacgtaccgc aatgggtaca atcagcgccg
    34741 ccgatccgag gtagatcagc gcctgaacca gcgtatgact atccatgatg ggcctcctgc
    34801 cattccagca gacgttgctt atagtggcgc gcctgccctt cgagggtttc gtcgtcacaa
    34861 ataaaggtgc agtgcatggc aaacggtggc agccagttca gcccgcagta gattgccgtc
    34921 gcctgtagcg gctgcgacag cacatcaaag cccggatgcg caccaatttc aaaatggctt
    34981 tccccgccgc cggtcgtcac cgcccacagc aaatgtttgc catgcagcgc cgtgccgccg
    35041 tgaccgtaag cccagccgtg cgacaaaact ttatcgatcc aaagtttaag aagcggagga
    35101 atgctgtacc actgcatcgg gtgctgccag acgatcagat cggcgcgaga gagcgcctcc
    35161 tgctcggcgg caatatcgat attgaagtca ggatagagtt gataaagaga gcgaatttcg
    35221 acgccttcca gcgtccttgc ctgttcaagc atccgtttat tcgcatggga atgatgcgga
    35281 tacggatgcg cataaatgat gaggatcata gttattagcc cgcgtgttta cgcctttgtt
    35341 ttcccacaaa gtgtagtcat atattcacgc ggcttatagt caataatatt gattcagaaa
    35401 agagagaaat ctgaacaaat aatcccattc tctgcccgcg gatcgtcata ttgctggtct
    35461 gcgccagtga cattcctcga gtctccgggg caaacgctac ggaaatcaac aagccaaaca
    35521 gcgagatacc cgcccccatt agcatcgtgt tactgatacc gtaattattg ataaatatcg
    35581 gcagtgccca ggtcgaaaca atggtgccaa tacgacttaa ggacataatc acgcccacgg
    35641 cagaggcgcg gatatccgtc gggaagagtt cattaggata gagccactgc aaattacccg
    35701 gcccgccaga gaaaaaggca tacaccgcaa aggccatcac taccagccag atccccatat
    35761 ccgggattag ccccaaaacc gccaacgcca gcgtcatcat ggcaaagctg ccaatcaaca
    35821 atggacgccg tccggcagtg tttagccaca gcatcggcgg aatacagccg agcataaaga
    35881 acaggctaat caccacattc cctagtgccg cgtttttgcc aacccccaat cccaacgaac
    35941 caacgatttg cgggccaaag gtgtaaatgg cgaacattgg gatcacctgg caggtccaga
    36001 tggcggcaac aaacagaaca aaaggaaaat ggcggcgatt aaacagatcg cgaaaacggg
    36061 tttgctgcgg ctgctcttca tcgaaagcca ccggttcgcc aaacagtttt atcatcatct
    36121 cttcgcactc ttttactcgc cctttgcgta ataaccagcg gggagattct ggcagttcga
    36181 atcgaccaat caaaatcaac aaacaaggga tcgccgcgct acccagcatc cagcgccaac
    36241 caccttccac atcataaagc cagtagccga ccagatcggc gcaggtcgcg ccgacatacc
    36301 acatcgcggc aataaagctg atggaaaacg cccgctgacg ggtactggag aactcggtga
    36361 tcatcgaagt ggcgatggga taatctgcac cgatgacaat gccgatgagt acccgcatca
    36421 ccaacagttc gacgggggat gaaacacaca tcgtcgccac cgatatcacg ccgatggcga
    36481 tgatatcaat gaggaacatt ttgcgccgtc cgactttatc ggagatatag ccaaacagcg
    36541 atgtgccaac gaacagcccg gcgagcgttc ccgcgcccag caagccaatc cagtcagcgt
    36601 ccagtttcag cgccggagtc agttgctcca gcgccacgcc aatcattacc agtacataac
    36661 catccagaaa cggaccaccg cttccccaca gcaaaatgcg gcggtgaata gaggagaatt
    36721 tgagatcgtc aaagtttctg gacggttgca tgggttattc ctgttttttt gcaatgagag
    36781 tcagtacggc gttacgtatc aaaccaacat cagccgtaac ggaactccac accaaaggtg
    36841 ccgcgcgggt attcccactg ttccagcgcc gaccccagcc ccagaatgcg acaggtgccg
    36901 cactccagac atccggcgta atcgaagcgc acactgccgt catcctgctt cttgtacaga
    36961 cctgcggggc acgctttcac cagcagctcc agcacctgtt tatcagcatc ggccttcaca
    37021 acaatgtgcg gatgctcttc atcgacattg aatttattga cgcccagttt gacgtccaca
    37081 ttgacgggag aagtcataaa acggtcactc ctttcatgcc atccttgatc agattgatga
    37141 agcccacttt cttgccgtgg cggaggattt tcttgcgcat cagttccggc gcactgccgt
    37201 caatggtgaa caggtcacgc gccacgccca ccgccagttc cgggttgccg ctaaacatgc
    37261 gtgggttatc aaggaacgcc ggtagtttct ggtacatgcg catatcgcgc agcgggccac
    37321 tctccagatg ctgacgatat tccgcgagtt tttgcttact gaaatcgtcg cttttcatcg
    37381 ctgaaagcac ggtttttgct gcagcttccc cggcggcaat cgccagatcc ataccgcgaa
    37441 tggtaaaacc gaggttcata cacattccgg cggcatcacc ggcaatcaat acgccgtcac
    37501 cgaccagctc cggcagcatg ttgatgcctg cttccggcac tacgtgagcg gaatattcca
    37561 ccagcttgcc gcccgcgatc agcggtgcaa cggccggatg ctgtttgaaa tcttccagca
    37621 tttgcggcac cgattttttc gcgtcatgca gatgatgcag accacaaacc agccccagcg
    37681 acagggtatt ttcattggta taaaggaagc cgccgcccat caggccatcg gtgggtgatc
    37741 ccgcaaacag acaagccgcg ccctgattac cctgcaactg aaaacggtct tcgattaccg
    37801 acttcggtaa ctcgatcagt tccttcacgc caaccgccac atccgtcggt ttaacgcgtt
    37861 ttgccatccc caacttttcg gcaaggatgg agttcacccc atcagcaagg agcaccgttt
    37921 tcgcttcaat cacatcacca tcggcttcta caccgacgac tttgccatcg cgctgtacga
    37981 ggttatcgac gcggatcccg gtaattaact gcgcgcccgc ttcttcggcc tgctccatca
    38041 accaggcatc aaatttactg cgcaaaacgg agtaagaacg ctgggatggc gaggtttcgt
    38101 caccgttgca gtagtccata gtcatcgctg acttttccgt cataaacgcg agtttttcat
    38161 gggtgatcag gcgttctacg ggggcggaat cggcgaaacc tggaataatg tgttccagac
    38221 tgtgggcata gagacgcccg ccagtaacgt tcttggcacc tgcggaattg ccacgctcga
    38281 taactaacac ttgcgcccct tcgcgggcga gcaccagtgc ggcaaccgaa ccggcaagcc
    38341 ctgcaccgac gatgatggcg tcaaagatat cttcggacat aagagctccc tgtcagcggc
    38401 agagccgtaa tgcagccctg ccagagtgga tcaacgcgct aaagctgcgg tcagtgccgg
    38461 aaggatcttc acggcatcgc caacaatgcc gtaatccgcg tactggaaga tcggcgcatt
    38521 tttatcttta ttgatggcga aaatggtttg cgacgcgtta gcgccaacca tgtgctggat
    38581 ctacccggag atccccaccg ccaggtacag ttcaggtttc agcatcaggt tggagatacc
    38641 gacatagcgt tcgtgctcca tccatttttc gttttctgcc accggacgag aacaggccaa
    38701 ctccgcacct atcgccttgc aaagctgttc tgccagcgca atgttctctt tgctgccaat
    38761 gccgcgaccg acgctgacca ccagacgggc tttgtcgaga tcgacgctgt tgctctggcg
    38821 cgcctgggtt gccgtgcggg taatcgccac agccggagcc tgccactcca cggtgtgcgt
    38881 ttcgccagtg cgtgacgcgt ctggctgagc cgcatcgaac gtgccgctgc tgatggtcag
    38941 taccgcatac ggcgtggcaa tgcgttcttc gccaatcgcc agaccgccgt aaaccatgtg
    39001 tttcactgtt gctttaccgt cctgtacgct gacggtgctg gcatcgttag acaccgccgc
    39061 tttaaggcga taacccagtt ttgccgccag taatttgccg cgacgggtgt ttggcaacag
    39121 caccagaccg tcagcgccgt gctggcgaat agtgtcagcc atgacaccgg cgtaatcttc
    39181 gatcatccga tcgtccggtt tgccgtttaa tttccagaca tgattagcgc cgagctggat
    39241 tgcctgtgcg ccgtcggcat cattgaggac aaaggcattg atttgattag ctaaagcctg
    39301 cgcaccgttc atcagttccg gcagacgaga gggggtatcg ctgaataccc agacttgaga
    39361 aaacgtgttc atagcatccc ctgtaattaa atgactttgc gaagattttc agcaaatgcg
    39421 gcgatctgtt cttcgccgtc gccttcaatc acgatgcgct gacgctcgcg ctgtttcggc
    39481 gcggcaacct gttgttcaga ccaggcgtcc acggcgttaa aaccaatatc cgccgccgac
    39541 catacctgga ctggcttttt cgccgcgccg agaatggctt tcatcgaagg aatttgtggg
    39601 gagttgatat cagtggaaac agcaacaacc gcaggcagcg gaatgcttaa ggtttcggtt
    39661 tcatcttcca gttcgcgctc aacggtgagg gtatctgccg tcagggagat aattttgctg
    39721 acgccgttaa ctgccggaat attgaggatt tcgcccacca gcagaccaac ctgctgggca
    39781 taaaggtcgg aagaaccatc gccacagagg atcagatcaa agcctgcttt ctgggcggct
    39841 gcagccagtg cgctcgccgt ttgttgcggc agtgcctgct cgaactggtc atcaatcacc
    39901 acaatcagtt catccgggcc gcgcgatagc acatctttac gccctttggc gttggtcagg
    39961 gctttaccgc ccacacttaa ggctgtcacc tgcgcctctg ctgcctgttg ctttagctgg
    40021 caagccgctt caatagcgtt gagatcgtat tggcttattt tggcatcggc tttgctgaag
    40081 tctaatgaac catcagcatt attgaccgca atatcctgtt catcaggcac gcacttatag
    40141 caagtaataa tcttcattgc atctccagaa atcatgaagg aaacgtcgtt catatcggtg
    40201 gtgttaatta cagatatcac gccctttaat ttggaaataa aac
//
LOCUS       DAZPCS010000021        39978 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZPCS010000021 DAZPCS010000000
VERSION     DAZPCS010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39978
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.021"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..155
                     /locus_tag="ACHUQ7_001077"
     CDS             <1..155
                     /locus_tag="ACHUQ7_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR1134591.1"
                     /translation="EQKMLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQILTVSGG
                     GVQELN"
     gene            384..974
                     /gene="uidR"
                     /locus_tag="ACHUQ7_001078"
     CDS             384..974
                     /gene="uidR"
                     /locus_tag="ACHUQ7_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFR1134592.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1362..3065
                     /gene="uidA"
                     /locus_tag="ACHUQ7_001079"
     CDS             1362..3065
                     /gene="uidA"
                     /locus_tag="ACHUQ7_001079"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFR1134593.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWCESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     N"
     gene            3071..4096
                     /gene="uidB"
                     /locus_tag="ACHUQ7_001080"
                     /pseudo
     CDS             3071..4096
                     /gene="uidB"
                     /locus_tag="ACHUQ7_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501049.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            4135..5400
                     /gene="uidC"
                     /locus_tag="ACHUQ7_001081"
     CDS             4135..5400
                     /gene="uidC"
                     /locus_tag="ACHUQ7_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HFR1134594.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIVGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMVGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWYDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGAN
                     YRVTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(5626..7134)
                     /locus_tag="ACHUQ7_001082"
     CDS             complement(5626..7134)
                     /locus_tag="ACHUQ7_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFR1134595.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLGSFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(7235..8410)
                     /gene="manA"
                     /locus_tag="ACHUQ7_001083"
     CDS             complement(7235..8410)
                     /gene="manA"
                     /locus_tag="ACHUQ7_001083"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFR1134596.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            8609..10255
                     /gene="fumB"
                     /locus_tag="ACHUQ7_001084"
     CDS             8609..10255
                     /gene="fumB"
                     /locus_tag="ACHUQ7_001084"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFR1134597.1"
                     /translation="MSNKPFHYQSPFPLKKNDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            10398..11801
                     /gene="fumC"
                     /locus_tag="ACHUQ7_001085"
     CDS             10398..11801
                     /gene="fumC"
                     /locus_tag="ACHUQ7_001085"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFR1134598.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(11798..12727)
                     /gene="tus"
                     /locus_tag="ACHUQ7_001086"
     CDS             complement(11798..12727)
                     /gene="tus"
                     /locus_tag="ACHUQ7_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFR1134599.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(12803..14104)
                     /gene="rstB"
                     /locus_tag="ACHUQ7_001087"
     CDS             complement(12803..14104)
                     /gene="rstB"
                     /locus_tag="ACHUQ7_001087"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFR1134600.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALTMGGTVNCDISELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(14283..15602)
                     /locus_tag="ACHUQ7_001088"
     CDS             complement(14283..15602)
                     /locus_tag="ACHUQ7_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HFR1134601.1"
                     /translation="MLFLICSWGIFKTDQKSESLSKSVFIAIFLGAIIGISLHFIPET
                     SSETVIEWYNIAGNGYVNLLKLVAVPLIFISILSAINKLENSAGIGKMSLTIVACMLC
                     LVMIAGLVGLAIASAIGLDASAFGHMQSSLTSDDVSKTAAVSLPRLITSLIPTNLFLD
                     LTGARSVSVIGIVIFTILAGIALLKVKKDAPAEGEKLSAGINAIQIWVMKMVRIVIAL
                     TPYGVMALMAKVFSTYQLSQFTNLLGFIVACYLAVFVMFIVHSGILLLIGKNPVNYYK
                     TVWQVLTFAFVSRSSAASIPLAISALEKLGVPNTIANIAASFGSSMGQNGCAGIYPAV
                     MVAMIAPTMGIDPFSLQFLATLLPAIALGSIGVAGVGSGGTFAALIVLSTLNFPVALV
                     VIFIAIEPIVDMARTALNVNGSIMSGVISARLLKDRETKEQQSVTLN"
     gene            complement(15827..16570)
                     /gene="rstA"
                     /locus_tag="ACHUQ7_001089"
     CDS             complement(15827..16570)
                     /gene="rstA"
                     /locus_tag="ACHUQ7_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFR1134602.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
     gene            16699..17034
                     /locus_tag="ACHUQ7_001090"
     CDS             16699..17034
                     /locus_tag="ACHUQ7_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFR1134603.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(17031..17753)
                     /gene="folM"
                     /locus_tag="ACHUQ7_001091"
     CDS             complement(17031..17753)
                     /gene="folM"
                     /locus_tag="ACHUQ7_001091"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFR1134604.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(17790..19172)
                     /locus_tag="ACHUQ7_001092"
     CDS             complement(17790..19172)
                     /locus_tag="ACHUQ7_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR1134605.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(19358..20302)
                     /gene="ydgH"
                     /locus_tag="ACHUQ7_001093"
     CDS             complement(19358..20302)
                     /gene="ydgH"
                     /locus_tag="ACHUQ7_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFR1134606.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            20826..22358
                     /gene="pntA"
                     /locus_tag="ACHUQ7_001094"
     CDS             20826..22358
                     /gene="pntA"
                     /locus_tag="ACHUQ7_001094"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFR1134607.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            22369..23757
                     /gene="pntB"
                     /locus_tag="ACHUQ7_001095"
     CDS             22369..23757
                     /gene="pntB"
                     /locus_tag="ACHUQ7_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFR1134608.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(23782..24816)
                     /gene="tqsA"
                     /locus_tag="ACHUQ7_001096"
     CDS             complement(23782..24816)
                     /gene="tqsA"
                     /locus_tag="ACHUQ7_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HFR1134609.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            25227..25592
                     /gene="mdtJ"
                     /locus_tag="ACHUQ7_001097"
     CDS             25227..25592
                     /gene="mdtJ"
                     /locus_tag="ACHUQ7_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFR1134610.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            25579..25908
                     /gene="mdtI"
                     /locus_tag="ACHUQ7_001098"
     CDS             25579..25908
                     /gene="mdtI"
                     /locus_tag="ACHUQ7_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFR1134611.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVVYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(25947..26768)
                     /locus_tag="ACHUQ7_001099"
     CDS             complement(25947..26768)
                     /locus_tag="ACHUQ7_001099"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFR1134612.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(26868..26951)
                     /locus_tag="ACHUQ7_001100"
     CDS             complement(26868..26951)
                     /locus_tag="ACHUQ7_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1134613.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(27044..27352)
                     /gene="asr"
                     /locus_tag="ACHUQ7_001101"
     CDS             complement(27044..27352)
                     /gene="asr"
                     /locus_tag="ACHUQ7_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFR1134614.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQPAKPAAQPAA
                     "
     gene            27623..27727
                     /locus_tag="ACHUQ7_001102"
     CDS             27623..27727
                     /locus_tag="ACHUQ7_001102"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFR1134615.1"
                     /translation="MNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            complement(27776..29029)
                     /locus_tag="ACHUQ7_001103"
                     /pseudo
     CDS             complement(27776..29029)
                     /locus_tag="ACHUQ7_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            29136..30038
                     /locus_tag="ACHUQ7_001104"
     CDS             29136..30038
                     /locus_tag="ACHUQ7_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1134616.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPYDHPLANNPSVTLAELAELAKEPFVFFDPHVGTGLYDDILGLMRR
                     YHLTPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWL
                     VWPKHHEQSPAARNFRIHLLNALR"
     gene            30173..31393
                     /gene="mlc"
                     /locus_tag="ACHUQ7_001105"
                     /gene_synonym="dgsA"
     CDS             30173..31393
                     /gene="mlc"
                     /locus_tag="ACHUQ7_001105"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFR1134617.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            31518..32213
                     /gene="bioD"
                     /locus_tag="ACHUQ7_001106"
     CDS             31518..32213
                     /gene="bioD"
                     /locus_tag="ACHUQ7_001106"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFR1134618.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKRVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(32166..33458)
                     /gene="clcB"
                     /locus_tag="ACHUQ7_001107"
     CDS             complement(32166..33458)
                     /gene="clcB"
                     /locus_tag="ACHUQ7_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFR1134619.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSSWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAIVAWLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(33617..34231)
                     /gene="dmsD"
                     /locus_tag="ACHUQ7_001108"
     CDS             complement(33617..34231)
                     /gene="dmsD"
                     /locus_tag="ACHUQ7_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFR1134620.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(34274..35128)
                     /gene="ynfH"
                     /locus_tag="ACHUQ7_001109"
     CDS             complement(34274..35128)
                     /gene="ynfH"
                     /locus_tag="ACHUQ7_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFR1134621.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(35130..35747)
                     /gene="ynfG"
                     /locus_tag="ACHUQ7_001110"
     CDS             complement(35130..35747)
                     /gene="ynfG"
                     /locus_tag="ACHUQ7_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFR1134622.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(35758..37796)
                     /locus_tag="ACHUQ7_001111"
                     /pseudo
     CDS             complement(35758..37796)
                     /locus_tag="ACHUQ7_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex A
                     subunit"
     gene            complement(37857..38891)
                     /locus_tag="ACHUQ7_001112"
                     /pseudo
     CDS             complement(37857..38891)
                     /locus_tag="ACHUQ7_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
     gene            38949..>39978
                     /locus_tag="ACHUQ7_001113"
     CDS             38949..>39978
                     /locus_tag="ACHUQ7_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFR1134623.1"
                     /translation="MTDINHDNTVYPSSSFSGSPYRYQQVYTSSPDTRLTQINLLSVA
                     KPQLVRRANGRYTIRFRLKGQTTPFLSVSTRSTDRRVATMRQRELAATAKAFMLDRPE
                     VSLQELTEHLRSMAEQFLTDASDDYWNGLEVATLVDEKSNLKELAATQALSLDQQKGI
                     RLALEVLTAAQQRVDTGDTSGLIKLIDDNNLTDDSTIGDSTSILNNEQGDRPAVFTQE
                     RQSSVVFSSLVSSLLAEKVQTLKTSSYKDLSSSLNTVSRFLPEDMDLMSRSGWLAVRD
                     SMLASEVRPSTINKLLTKAKMCLDYGLMNGQLEGRNPIERMKLTKDIDSKRRAFTDEE
                     LERLLVRVE"
BASE COUNT        10163 a         9697 c        10237 g         9881 t
ORIGIN      
        1 ttgaacaaaa aatgttacag cacacgccga tcagacgtct gggccaaccg caagatattg
       61 ctaacgcggc gctgttcctt tgctcgcctg cagccagctg ggtaagcgga caaattctca
      121 ccgtctccgg tggtggggta caggagctca attaatacac taacgaaccg gtaaacggtc
      181 gtatgtgttg tttaccggga tagcgtgtgc aattcatcaa cgtctctgct aaacaactgg
      241 cagccaaatc acggctattg gttaaccaat tgctgggtga aaagtatacg aatagagtgt
      301 accttcgcac tattcaacag caatgatagg cgctcacctg acaacgcggt aaactagtta
      361 ttcacgctaa ctataatggt ttaatgatgg ataacatgca gactgaagca caaccgacac
      421 ggacccggat cctcaatgct gccagagaga ttttttcaga aaatggattt cacagtgcct
      481 cgatgaaagc catctgtaaa tcttgcgcca ttagtcctgg gacgctctat caccatttca
      541 tctccaaaga agccttgatt caggcgatta tcttacagga ccaggagagg gcgctggctc
      601 gtttccggga accgattgaa gggattcatt ttgttgacta tatggtcgag tctattgtct
      661 ctctcaccca tgaagccttt gggcaacggg cgctggtggt cgaaattatg gcggaaggga
      721 tgcgtaaccc acaggtcgcc gccatgctta aaaataagca tatgacgatc actgaatttg
      781 ttgcccagcg gatgcgtgat gcccagcaaa aaggcgagat aagcccagac atcaacacgg
      841 caatgacttc acgtttactg ctggatctga cctacggtgt actggccgat atcgaagcgg
      901 aagacctggc gcgtgaagcg tcgtttgctc agggattacg cgcgatgatt ggcggtatat
      961 taaccgcatc ctgatcctct catttcgcgg gctggtgata actgtgcccg cgtttcatat
     1021 cgtaatttct ctgtgcaaaa attatcctcc ccgtgttcgg agaattcccc cccaaaatat
     1081 tcaccgtagc catatgtcat gagagtttat cgttcccaac actctcgaac gaacgttcga
     1141 ttgcttattt tatggcttct gtcaacgctg ttttaaagat taatgcgatc catatcacgc
     1201 tgtgggtatt gcagtttttg gttttttgat cgcggtgtca gttcttttta tttccatttc
     1261 tcttccatgg gtttctcaca gataactgtg tgcaacacag aattggttaa ctaatcagat
     1321 taaaggttga ccagtatttt tatcttaatg aggagtccct tatgttacgt cctgtagaaa
     1381 ccccaacccg tgaaatcaaa aaactcgacg gcctgtgggc attcagtctg gatcgcgaaa
     1441 actgtggaat tgatcagcgt tggtgcgaaa gcgcgttaca agaaagccgg gcaattgcag
     1501 tgccaggcag ttttaacgat cagttcgccg atgcagatat tcgtaattat gtgggcaacg
     1561 tctggtatca gcgcgaagtc tttataccga aaggttgggc aggccagcgt atcgtgctgc
     1621 gtttcgatgc ggtcactcat tacggcaaag tgtgggtcaa taatcaggaa gtgatggagc
     1681 atcagggcgg ctatacgcca tttgaagccg atgtcacgcc gtatgttatt gccgggaaaa
     1741 gtgtacgtat caccgtttgt gtgaacaacg aactgaactg gcagactatc ccgccgggaa
     1801 tggtgattac cgacgaaaac ggcaagaaaa agcagtctta cttccatgat ttctttaact
     1861 atgccgggat ccatcgcagc gtaatgctct acaccacgcc gaacacctgg gtggacgata
     1921 tcaccgtggt gacgcatgtc gcgcaagact gtaaccacgc gtctgttgac tggcaggtgg
     1981 tggccaatgg tgatatcagc gttgaactgc gtgatgcgga tcaacaggtg gttgcaactg
     2041 gacaaggcac cagcgggact ttgcaagtgg tgaatccgca cctctggcaa ccgggtgaag
     2101 gttatctcta tgaactgtgc gtcacagcca aaagccagac agagtgtgat atctacccgc
     2161 tgcgcgtcgg catccggtca gtggcagtga agggcgaaca gttcctgatt aaccacaaac
     2221 cgttctactt tactggcttt ggtcgtcatg aagatgcgga tttgcgaggc aaaggattcg
     2281 ataacgtgct gatggtgcac gatcacgcat taatggactg gattggggcc aactcctacc
     2341 gtacctcgca ttacccttac gctgaagaga tgctcgactg ggcagatgaa catggcatcg
     2401 tggtgattga tgaaactgca gctgtcggct ttaacctctc tttaggcatt ggtttcgaag
     2461 cgggcaacaa gccgaaagaa ctgtacagcg aagaggctgt caacggggaa actcagcagg
     2521 cgcacttaca ggcgattaaa gagctgatag cgcgtgacaa aaaccaccca agcgtggtga
     2581 tgtggagtat tgccaacgaa ccggataccc gtccgcaagg tgcacgggaa tatttcgcgc
     2641 cactggcgga agcaacgcgt aaactcgacc cgacgcgtcc gatcacctgc gtcaatgtaa
     2701 tgttctgcga cgctcacacc gataccatca gcgatctctt tgatgtgctg tgcctgaacc
     2761 gttattacgg ttggtatgtc caaagcggcg atttggaaac ggcagagaag gtactggaaa
     2821 aagaactgct ggcctggcag gagaaactgc atcagccgat tatcatcacc gaatacggcg
     2881 tggatacgtt agccggcctg cactcaatgt acaccgacat gtggagtgaa gagtatcagt
     2941 gtgcatggct ggatatgtat caccgcgtct ttgatcgcgt cagcgccgtc gtcggtgaac
     3001 aggtatggaa tttcgccgat tttgcgacct cgcaaggcat attgcgcgtt ggcggtaaca
     3061 attgacctac atgggcctcg ggctttgcta cagcctggtg aatattcctt atggttcact
     3121 tgctaccgcg atgacccaac aaccacaatc tcgcgcccgt ctgggcgcgg ctcgtgggat
     3181 tgccgcttca ttgacctttg tatgcctggc atttctgata ggaccgagca ttaagaactc
     3241 cagcccggaa gagatggtgt cggtatacca tttctggacg attgtgctgg tgattgccgg
     3301 aatggtgctt tacttcatct gcttcaaatc gacgcgtgaa aatgtggtac gtatcgtggc
     3361 gcagccgtca ttgaagatca gtttgcaaac cctgaaacgg aatcgcccgc tgtttatgtt
     3421 gtgcatcggt gcgctgtgtg tgctgatttc gaccttcgcg gtcagcgcct cgtcgttgtt
     3481 ctacgtgcgc tatgtgttaa atgataccgg gctgttcact gtgctggtac tggtgcaaaa
     3541 cctggttggt actgtggcat cggcaccgct tgtgccgggg atggtcgcga ggatcggtaa
     3601 aaagaatacc ttcctgattg gcgctttgct gggaacctgc ggttatctgc tattcttctg
     3661 ggtttccgtc tggtcactgc cggtggcgtt ggttgcgttg gccatcgctt caattggtca
     3721 gggcgttact atgactgtga tgtgggcact ggaagctgat accgtagaat acggtgaata
     3781 cctgaccggc gtgcgaattg aagggctcac ctattcacta ttctcattta cccgtaaatg
     3841 cggtcaggca atcggaggtt caattcctgc ctttattttg ggattaagcg gatatatcgc
     3901 caatcaggtg taaacgccgg aagttattat gggcatccgc acatcaattg ccttagtacc
     3961 ttgcggattt atgctactgg cattcgttat tatctggttt tatccgctca cggataaaaa
     4021 attcaaagaa atcgtggttg aaattgataa tcgtaaaaaa gtgcagcagc aattaatcag
     4081 cgatatcact aattaatatt caataaaaat aatcagaaca tcaaaggtgc aactatgaga
     4141 aaaatagtgg ccatggccgt tatttgcctg acggctgcct ctggccttac ctctgcttac
     4201 gcggcgcaac tggctgacga tgaagcggga ctacgcatca gactgaaaaa cgaattgcgc
     4261 agggctgata agcccagtgc tggcgcggga agagatattt acgcatgggt acagggagga
     4321 ttgctcgatt tcaatagtgg ttattattcc aatattgttg gcgttgaagg cggagcgtat
     4381 tatgtttata aattaggtgc tcgtgctgat atgagtaccc ggtggtatct tgatggtgat
     4441 aaaagttttg gctttgccct gggggcagta aaaataaaac ccagtgaaaa tagcctgctt
     4501 aaattaggtc gcttcgggac ggattatagt tatggtagct taccttatcg tattccgtta
     4561 atggttggca gttcgcaacg tacattaccg acagtttctg aaggagcatt aggttattgg
     4621 gctttaacgc caaatattga tctgtgggga atgtggcgtt cacgagtatt tttatggact
     4681 gattcaacaa ccggtattcg tgatgaaggg gtgtataaca gccagacggg aaaatacgat
     4741 aaacatcgcg cacgttcttt tttagccgcc agttggtatg atgataccag tcgctattct
     4801 ctgggggcat cggtacagaa agatgtttcc aatcagatac aaagtattct cgagaaaagc
     4861 ataccgctcg acccgaatta tacgttgaaa ggggagttgc tcggctttta cgcgcagctc
     4921 gaaggtttaa gtcgtaatac cagccagccc aatgaaacgg cgttggttag tggacaattg
     4981 acctggaatg cgccgtgggg aagcgtattt ggcagtggtg gttatttgcg ccatgcaatg
     5041 aatggtgccg tggtggatac cgacattggc tatccctttt cattaagtct tgatcgtaac
     5101 cgtgaaggaa tgcagtcctg gcaattaggt gccaactacc gtgtaactcc gcaatttacg
     5161 ctgacatttg caccgattgt gactcgcggc tatgaatcca gtaaacgaga tgtgcggatt
     5221 gaaggcgcgg gtatcttagg tggtatgaac tatcgggtca gcgaagggcc gttacaaggg
     5281 atgaatttct ttcttgctgc cgataaaggg cgggaaaagc gcgatggcag tacgctgggc
     5341 gatcgcctga attactggga tgtgaaaatg agtattcagt atgactttat gctgaagtaa
     5401 gaaataacgc cggagagaaa aacctccggc gtttcagatt gttgacaaag tgcgcgtttt
     5461 ttatgccgga tgcggcgtaa acgccttatc cagcctacaa aaactcataa attcaaagtg
     5521 ttgcaggaaa aggtaggcct gataagcgta gcgcatcagg caatctctgg tttgttttca
     5581 gatgaaaacg ccggagtgaa aattctccgg cgttttggcc gtgaattact gctgcggaat
     5641 tgccggtaca gccggaacgt taagagctgg catcgcaaac atgccaacaa aatcttctaa
     5701 cgacattttc tgcccattta acgttatctg accgttagca tattgcaggc tggtggtgat
     5761 ggtattgtcc tgcaaggtgg tcagacggaa catctgcccc attgctgatg caccttcaac
     5821 ttgctgtttc gccagttttt tcgcttgatc ttcctgataa ccttccagct tcgctacctg
     5881 agtcatcaac tcagttgcca tatccaccgg aatggtcagt ttcgcatcca gagatttaac
     5941 cgaacgatct acttcctgcg ccagcgtttg cggcgcttct ttagtcgttg ccggatcttt
     6001 caggaacagc gacagattca gcgcactttc accctgactg tttttccagc ttagcggcgc
     6061 gatagtaatc accggatcgc ctttcaacat cagcggcagg gcgctaaaga aggcttccgt
     6121 cactttctcc tgataaagtt cgggattgtt ggcaatttct ggctgtgcca gcagcgcctg
     6181 agtttgcgcg ttatattgct ggctaaactg atgccaggct tcaccatcaa tctggccgac
     6241 ttttaaagtc agcttgccgc tgcccagatc ctgattctgt accttcaggc tgtttagcga
     6301 gtaatccagt tggctattga tcgttttacc gtcattgacc agatccgatt taccgctgat
     6361 ctccatgcct tccagcagtg ccagttcttt gccttccact gaaatggtca ttttttccag
     6421 tgacagtttt tggtttccta cgcgctcacc aaaacttccc agcgtgctgg aaccgtcggt
     6481 tttcagatta ttaaaggtca actgcacttt ctggttgtat tcgttaactg cgtctatccg
     6541 accactttgc gcctctccgg aaagggagat ggctttgccg tctctgtcag catttaactg
     6601 gaactcgccg ccgctaaagg cgactttttc atccttttgc tcgtaattca gtggattgag
     6661 cgaaatatcg gaactggaat caccgctgta accaatgcgc gagttaatct caaaaggcgt
     6721 ttcacctttt gccatatcaa acagtggttt gcttacttcg ttattaacca gcgtggtttg
     6781 aattgatgcc atcgacggga tcaggttcag ttttttaagc tgggcaagcg ggaagggacc
     6841 atgatcaacc gattcgttga agatgacgct ctgaccgctt ttaatccacg gattttcttt
     6901 cccggcaatg ggtttcacca acagttgcaa ctggctgctg aatacgccgc gatgatagtt
     6961 ttgataactc acttccaggt tggattcagg agctgtcagt ttgagttgcg cgttcgcctg
     7021 cgcgaccatg tcttcgagat gggtttcaat cttcttgcct gtataccatg cgccgcctgt
     7081 ccagactacg cctagcgcaa caatgacgcc taccgctacc agcgatttat tcataatgat
     7141 tatccataaa atgaaatcag gcggactggc cgcctgaagg tgttataagc ctttaataag
     7201 cttagcaaga gatgttaatt ttttcagtaa gctcttacag cttgttgtaa acacgcgcta
     7261 aacggccgtg gcctttgaca gtcaccggtg attcgttggc ggcaataaac gctgattcac
     7321 ccggtttaag ctgtaactgc tgagaacctt tccacaacgt tgcatcgcct tcgacgcaga
     7381 acaaaatggc ggcactctgc tggctgatgg tggtttcttt atcactaagg tcatgcagcg
     7441 agaaggcaaa atcatccaca ggaatcggga agtccagttc tgcaccttgt ttcaccggct
     7501 gggtcaacaa ctggttagcc ggtttggctt cgaatttcac attggcaacc agttccggaa
     7561 tatcaatgta tttaggcgtc agacctgcac gcagcacgtt atcggagttc gccatcactt
     7621 ccagcgccac gccttgcagg taagcgtgcg gtgtttcagc gaacaggaac atcgcttcgc
     7681 caggattcaa tttcaccaca ttcagtaaca gcggggagaa caggccgctg tcttccgggt
     7741 aaaattcaga aattaaacga atcgtttgcc acggttcacc ctgctgactg tcgagcgccg
     7801 attttaaaat cgccagcgcg cgggattttt cttcaccctg catattcaac aggttggcga
     7861 acagttcgct taaacgttcg gcatgtggct gttgtaaaaa gtgagcaatc gccggatgtg
     7921 caccggcgac cggctggagt agggagacaa tctcggaaaa ttcacgaaac gcgttcatcg
     7981 caaggaaagg cgtcagcgca aaaaccagct ccggcttgtg gttaggatct ttatagttac
     8041 gctcggcggc atccatcggg atacctgcgg cattttcttt ggcaaaaccg atttcagaat
     8101 tgtgtttgtt tggatgaacc tgaatggaga gtggctgtgc tgcgcataat actttgaaca
     8161 ggaaaggcag ttcgccaaag cgtttggcaa cggcctctcc gagcagagtc gatttatcac
     8221 tctcaatcac atcacgcagt gaaacgatat ctccggcggc attctgcact cgtgaactgc
     8281 ttttcggatg tgcgcccatc cacagctcgg ccatcggctg gctggacgga ttttccatac
     8341 cataaagttc agtcaacgcc gttttgctac cccaggcata gttttgcact gagttaatga
     8401 gtttttgcat gatcaatccc tgttttaatg tggaaattaa tcccgctatt aaagcaagaa
     8461 tcctacggga agtaacctgg agccgcaaaa agtcgtacta gtctcagttt ttgttaaaaa
     8521 actgtgtagg atattgttac tcgcttttaa cagggcaacg gaacacccgc ccagagcata
     8581 accaaaccag gcagtaagtg agagaacaat gtcaaacaaa ccctttcatt atcagtctcc
     8641 ttttccactc aaaaaaaatg atactgagta ttacctgcta accagtgaac acgttagcgt
     8701 atctgaattt gaagggcagg agattttgaa agtcgcaccc gaagcgttaa ctctgttggc
     8761 gcgtcaggcg tttcatgatg cgtcgtttat gctgcgtccg gctcaccaac aacaggtggc
     8821 cgacattctg cgtgacccgg aggccagcga aaatgataaa tatgtggcgc tgcaattcct
     8881 gcgtaactcc gacatcgcgg cgaaaggcgt tctgccaacc tgtcaggata ccggcaccgc
     8941 gattattgtt ggtaaaaaag ggcagcgtgt atggaccggt ggtggtgatg aagcggcgct
     9001 ggcgcgcggt gtctataaca cttatatcga agataatctg cgctactcgc aaaacgcgcc
     9061 gctggatatg tataaagaag tgaataccgg caccaatctg ccagcgcaga tcgatcttta
     9121 tgccgttgat ggcgacgaat acaaattcct ctgtatcgca aaaggtggtg gttcggcaaa
     9181 caagacgtat ctctatcagg aaaccaaagc gttactgacg ccggggaaac tgaaaaatta
     9241 cctggttgag aagatgcgca cgctgggtac cgcggcctgt cctccgtatc atattgcgtt
     9301 cgttattggt ggaacttctg cagaaacgaa ccttaaaacg gtgaaactgg cttccgcgaa
     9361 atactatgat gaactgccaa cggaagggaa tgagcacggt caggcgttcc gcgatgtgga
     9421 actggaaaaa gaattactga tcgaagcgca aaatctcggt ctgggtgcgc agtttggtgg
     9481 taaatacttc gctcacgaca tccgcgtgat tcgcctgcca cgtcacggcg catcctgtcc
     9541 ggttggtatg ggtgtttcct gctctgctga ccgtaatatc aaagcgaaga tcaaccgtca
     9601 ggggatctgg atcgaaaaac tggaacataa tccaggtaaa tatatccctg aagagctgcg
     9661 taaagcggga gaaggcgaag cggtgcgcgt tgaccttaac cgtccgatga aagagatcct
     9721 cgcacagttg tcgcagtatc ccgtttctac acgcttatcg cttaacggca cgattatcgt
     9781 tggtcgtgat attgctcacg ccaaactgaa agagcggatg gataacggtg aagggctgcc
     9841 gcaatacatt aaagatcatc cgatttacta cgcgggtccg gccaaaacgc cggaaggtta
     9901 tgcctccggt tctcttggcc caacgaccgc cggacggatg gattcttatg tcgatcaact
     9961 gcaagcgcag ggcggaagta tgatcatgct ggcgaaaggc aaccgcagcc agcaggtgac
    10021 ggatgcctgt aaaaaacacg gcggcttcta ccttggcagt atcggtggtc cggccgctgt
    10081 attggcgcag ggaagtatta agagcctgga atgtgttgaa tatccggaac tgggaatgga
    10141 agccatctgg aaaattgaag tggaagattt cccggcgttt atccttgtgg atgataaagg
    10201 aaatgacttc ttccagcaga tacaactcac acagtgcact cgctgtgtga aataaacaga
    10261 gccgcccttc ggggcggttt ttttacatgg cacgaaagac caaacatttg ttatcaaata
    10321 gtaaataatg agtgagctaa aagttgctta acgaaagcaa aacagaaaga aaaaattaat
    10381 caggtgagga gcaggccatg aatacagtac gcagcgaaaa agattcgatg ggggcgattg
    10441 atgtcccggc agataagctg tggggcgcac aaactcaacg ctcgctggag catttccgca
    10501 tttcgacgga gaaaatgccc acctcactga ttcatgcgct ggcgctaacc aagcgcgcag
    10561 cggcaaaagt taatgaagat ttagacttgt tgtctgaaga gaaagcgagc gccattcggc
    10621 aggcggcgga tgaagtactg gcaggacagc atgacgacga attcccgctg gctatctggc
    10681 agaccggctc cggcacgcaa agtaacatga acatgaacga agtgctggct aaccgggcca
    10741 gtgaattact cggcggtgtg cgcgggatgg aacgtaaagt tcaccctaac gacgacgtga
    10801 acaaaagcca aagttccaac gatgtctttc cgacggcgat gcacgttgcg gcgctgctgg
    10861 cactgcgcaa gcaactcatt ccgcagctta aaaccctgac acagacgctg agtgaaaaat
    10921 cgcgtgcatt tgccgatatc gtcaaaatcg gtcgaaccca cttgcaggac gccacgccgc
    10981 taacactagg gcaggagatt tccggctggg tagcgatgct cgagcataat ctcaaacata
    11041 tcgaatacag cctgcctcac gtagcggaac tggctcttgg cggtacagcg gtgggtactg
    11101 gactaaatac ccatccggag tatgcgcgtc gcgtagcaga tgaactggca gtcattacct
    11161 gtgcaccgtt tgttaccgcg ccgaacaaat ttgaagcgct tgcgacctgt gatgccctag
    11221 ttcaggcgca cggcgcgttg aaagggttgg ctgcgtcact gatgaaaatc gccaatgatg
    11281 tccgctggct ggcctctggc ccgcgctgcg gaattggtga aatctcaatc ccggaaaatg
    11341 agccgggcag ctcaatcatg ccggggaaag tgaacccaac ccagtgtgaa gcattaacca
    11401 tgctctgctg tcaggtaatg gggaacgacg tggcgatcaa catgggtggc gcttccggta
    11461 actttgaact gaacgtcttc cgtccgatgg tgatccataa tttcctgcaa tcggtgcgct
    11521 tgctggcaga tggcatggaa agttttaaca aacactgcgc agtgggtatt gaaccgaatc
    11581 gtgagcgaat caatcaatta ctcaatgaat cgctgatgct ggtgactgcg cttaacaccc
    11641 acattggtta tgacaaagcc gcggagatcg ccaaaaaagc gcataaagaa gggctgacct
    11701 taaaagctgc ggcccttgcg ctggggtatc ttagcgaagc cgagtttgac agctgggtac
    11761 ggccagaaca gatggtcggc agtatgaaag ccgggcgtta atctgcaaca tacaggtgca
    11821 gccgtggaat gatcaaacga agcggctgcg cctgaggttt ataacggtgc tgcacattgt
    11881 cggcatcgta atttaacaac tcaccaacgt ccggcacgcc cgcgccatta tcccgattaa
    11941 tcagtgcaat cagtggtgta gggcaggcgt gttggacttg tttttgatct cctttgtacc
    12001 agacgcgggc aatcggctgc accttcactg gacgtttgat ttttaacttc gcgttctgtg
    12061 gcagggcagc gatatcctga tactctcgct ccagttttct ttgccactcc tcgcgcgtcc
    12121 acggtgcgac actgcgtggt gatttcaggc ttttttccag ctgtgccagg acttcatcac
    12181 gatgcaaatt cttaatgata tgtttattag cccaaccaaa gcgtaaagtg gcggggtcgt
    12241 gcagaacggt gagcgtgcgg taagcattaa gggtgatcag ccccggcaaa tgacgatgca
    12301 cccattcaaa acgtgccgcg gtggggagtt ctgattcaac cgtgacgata tgctcgaacg
    12361 tggtcttgag tttattgatg tgctgaatat gactgaccaa cgctgcttgc gaaaggttat
    12421 cgacctggta acacaacacg ccaggcagac ggacagcggc cttgctgctg cgattttcgg
    12481 actgttgttg aataaataaa tggcggaaat gacgcaacgc cagcgactgc gcgtcgttgc
    12541 cgagatgttg ttttacctca atacgattaa gcggattatg ctcatcctct ttttttacct
    12601 ccggcaaaga gaacacgcgg gcaaccaata gcttgtgttg ctcaagatga gcggcaaatg
    12661 tagccagctc ttgttccatc tggcgaaagg tagtgttgag tcggtctacg agatcgtaac
    12721 gcgccataat attaaccact ttagttacaa catacttatt ttatagcaca gtcgtggtga
    12781 cttgaccatc gtgcgcgtag tgtcaggcag aggtaaattg cgggatgtta tgccataacg
    12841 gccagctaaa cgagaagcga gcaccaccca gttcgctgat gtcacaatta accgtaccgc
    12901 ccattgtcag tgctattgag tggacaattg ccagccccag cccgcagccg ccggttgagc
    12961 gatcccggct gggatcgagg cgaacaaaag gttcaaagat atgttcgcgg ttttctgggg
    13021 caatccctgg gccatcatcc tcaacaatta atgtcgctct attccccgac agtagcaggc
    13081 tggtttcaac cgttgaatgg cagtagcgca gggcgttatt gagcaaatta tccagcacgc
    13141 gctccattaa gcgcatatcc aacgccgcat aatggccttg catgagcgtt tttatccgta
    13201 ccgttttatc gggcgttact gcctgaatat ctgccagatg cgttgacagc cacaacggca
    13261 ggtctggttc gctaagatga agctcgtttt gtgggcgatc gagtcgggca taagtcagca
    13321 gctcttcaat taaagcttca agttgactga tatcacgatt caacgcctgg gattcggcgg
    13381 cgctcaggtt atcgctcatc tccagtcgat aacgcaggcg cactaacggt gttcgcagtt
    13441 cgtgagcgat accgtcaata agctgttttt tgctggcaat taaggcgttg atattgtccg
    13501 ccatctggtt aaatgcgata ccaagtcgtt caaagctcga accctcatca aagtggatac
    13561 gttcattgag atgcccatcg ccaaatcgtt gcgccgctgc ttccagtttt aacatatcct
    13621 gccagtgcgg acgcatccag ataaacaccg gaaaggcgag ggaaatagca ataaaagcga
    13681 tcagggcgat atccagcaat cgcatttgat ggaggtaata aagataagga acaggaccaa
    13741 ctgccagcac gtagtggctg cgcgggatac gctgcaaaaa cgtgtactga tcgtccaggg
    13801 cgacaatttc gccgccacgc agtcggtgca tggaaatatc atcaagatgg tatttactca
    13861 gtggctcgac acgcagatcg aaagagagat ttaaatccat ctctttcagc gtcttacccc
    13921 agtcgtgtgg ggggatctct cgcaattcgc tgcgcatcag atacagcgaa ctgttcatca
    13981 aatcatccag cgactgtttg cccgcgcgtt cggcggtaaa tttgtacacc agcccaacca
    14041 gcagagacat cacaaggaag cagacaaaca ataacaggta aaactggata aacaattttt
    14101 tcattggcat cgcccgcaac agaatactat tcgtattaat tcacgactgt ggagcaatgt
    14161 aaaccgccaa ctggataaga ccttgtttgg aagataatgt ccggaacaga cggatgtatc
    14221 cccggtgtat tcatattcag taaattcaat tttccaccgg ggcttgttta ttatgctaag
    14281 gtttaattta acgtgacgct ttgttgttct ttagtctctc tatccttaag aagacgggct
    14341 gatattacac cggacattat tgaaccattc acattaagcg ctgttcttgc catatcaacg
    14401 atcggctcta tggcaataaa aataactact aatgcgacag ggaaattaag tgttgaaagg
    14461 acaatcagcg ccgcgaatgt cccaccacta cctacgcctg ctacccctat tgagccaagc
    14521 gcgatggcag gtaaaagagt tgcgaggaat tgcaatgaga agggatcgat acccattgtt
    14581 ggagcaatca tcgccaccat cacggcagga taaatcccag cgcagccatt ttgtcccatg
    14641 ctggaaccaa aagacgctgc aatattagca atcgtatttg gaacccccaa tttttccaat
    14701 gcagaaatag ccagaggaat tgaagccgca ctactgcgag aaacaaacgc aaaggtaagt
    14761 acttgccaga cggtcttgta atagttaacg gggtttttac ctattaacag caaaatgccc
    14821 gagtgaacaa taaacatcac aaaaacggcc agataacagg cgacgatgaa cccgagcaaa
    14881 ttggtaaact gtgaaagctg atacgttgag aagactttcg ccattagtgc cattacccca
    14941 taaggcgtca gcgcaatgac aatacggacc attttcatta cccatatttg aatcgcgttg
    15001 atccccgcgc tcaatttttc gccctctgct ggtgcatctt tctttacttt taacagcgca
    15061 ataccagcaa gaattgtaaa gatgacgatg ccaataaccg aaacacttct tgccccggtc
    15121 agatcaagga agaggttggt tgggatcaat gacgtaatga gtcgcggtaa agataccgca
    15181 gctgttttcg agacgtcatc gctggttaaa gaggattgca tatgaccaaa ggcgctggca
    15241 tcaaggccaa ttgcactggc aatagccagg ccaactaaac ctgcaatcat gactaaacac
    15301 aacatacagg caacaatagt taaagacatc ttgccaatac cagcgctatt ttcgagctta
    15361 ttaatcgctg aaagaatcga aatgaatatg agtggaacgg caacaagttt taataaatta
    15421 acataaccgt taccagcaat attataccat tcgataacgg tttcactgct tgtctcagga
    15481 atgaaatgca gggagatacc aatgatagcc cccaggaaaa tcgcgataaa aacacttttg
    15541 gaaagggatt cgctcttttg atccgttttg aaaatccccc atgagcagat caaaaaaagc
    15601 acaatatatg tgaaagttgt aaccgttttt taagcctctt gcctcattca gcacctgaaa
    15661 tgccggaaag agtttgttgt aaaaaatctg cgccataaga cgcagggacg aggcgattat
    15721 agagaaattt tttctcattt tcatatcaaa tgtagggggt ttggaatttg aataagctct
    15781 tattaattga attcgttaat tgttctcttt ttcagatgaa caaatattag tcatctgatt
    15841 gtttattgtc actatcccat gcatgaggcg caaaaagata gcctttgtta cgcacagttt
    15901 taatgcgata aggttctgcg gcgttatcga gcagtttttt tcttaaccgc gaaatagcca
    15961 cgtccacgct acgatccagt ccgtcataac tgacgccgcg taaatttttc agcaatgcat
    16021 cgcggtccat gatttgcccg gcatgcgtag ctagttccca caataattcg aaatcagctg
    16081 tcgagagcga gatttcagtg ttagccaggg tgactacgcg gttgatgggg tcgatagtta
    16141 acgtgccgaa atgcagggct ttgtagggag taagagacgt ttcctgaaga cctttggtca
    16201 gtgtggcttg ctcattctga cgcaaatgca aacgtaaacg cgctagcaaa acagcagggg
    16261 gcgtcgtttt gagaatatag tcgcaggcac ccatttccag tgccaggatg tggttcatat
    16321 cgctatcgag agaggttaga agaacaatcg gtccagacca ctttgcgcgt aaatcacgac
    16381 aaatggtcat gccgtccttg cctggtagca tgatgtcgag taacaccaaa tccggatttt
    16441 ctcgcaaaat ggtttcttcg gcctggtcgc cgcgcggttc tacggtaacc tgcatatcat
    16501 gttttgccag gtacgcggca atcagtgaac cgacttccgc atcatcttcc acaaatacga
    16561 tagtgttcat aacattcacg gtagatataa aaacgtcaca atacaccgcg ctgtttttac
    16621 ttactatcaa tcgtttctaa acaagttttc atcaggtatt ctactgatga cttgttatgg
    16681 aagtgttaag gtaaaaagat ggggctggta atcaaagccg cgctcggtgc gctggtagtg
    16741 ctgttgattg gtgttttagc aaaaacgaaa aattattata tcgccgggct gattccactt
    16801 ttcccgacct ttgcgcttat cgcgcattat attgttgcca gcgaacgcgg cattgaagcc
    16861 ttacgcgcaa ccatcatttt tagtatgtgg tcgattattc cctattttgt ttacctggtg
    16921 tcgctgtggt atttcaccgg aatgatgcgc ttgcccgccg cttttgttgg ttctgttgcg
    16981 tgttggggga taagcgcatg ggtgttgatt atatgctgga taaagctgca ttaacgcaga
    17041 tgacgaccgc catcaagtgg gaaactgcgt ccggtgacaa agcaactggt aagtaagtaa
    17101 tcgaccaggt cgatcacttc tttctcgcca ggcgcggttt tcatcagtga tttattcagc
    17161 gcctgttgtc gatattcggc atcatcatgt tcattaaaca ggatcagcga tggcgcaata
    17221 gaattcactt tcacttccgg tgccagcttg cgggcaaacg agcgggtcat attatccagt
    17281 gccgctttgc ttgcagcata cgcaacatgt ttgtcgctac cgcgctccac cacataatcg
    17341 gtaaagtgaa tgatatcgct ggcggcgtgt ccgtgcccac gcagtaatct ttccagcgca
    17401 tggttgagca ggtatggggt attaacgtgg atctgcatca tgcaagccag tacgtcggcc
    17461 agtggcgcac ccggtttttc cgcaatccac gcactggcgt tatgcaaaat agcacgcaga
    17521 ccatgggtgg tttttagtac ttcatcggca aacgccatca caccgtcgtt ggtcgaaaaa
    17581 tcagcctgaa tacactgcgc acctgcatta atcagtccat caatggctgg atagtgtgtc
    17641 cgatagctga caatcaccgg ttgcttttga ttaatgaaat gccatgcgag ggcgaggccg
    17701 atgcgacgac ctccgccagt aattaatatt ggcaagggct gggttttacc catcgttatc
    17761 tcctttgcta tccaacgatg ggatgaacgt tatcccacca gcatccaggt cgccggaact
    17821 gaggcaatta acagcatgcc aatcagcacc atttcctgac ggttcagtac attatcatgt
    17881 gtatgcgttt tgcgcgcata caggaaaacc agcagccccg gtgcatacag aacaacggat
    17941 aacagcaagt gcattggacc agaggcgtac aataaccata agccataaat gcaggcaccg
    18001 acacctaccg ctttatgcag tggacgggtt gcgattttca gcaagaacgc gccgaccagg
    18061 aaatagggca ccagaatcat ttctgaggcg atggtcagca acgtgttgta atcggaaccg
    18121 gtcagccaga tgagtaccag acaaatttgc acgcagatgt tggtcaacca cagtgaggcg
    18181 gatggcgcag cttgtgcgtt ctggcgcgca aaaatgcgtg ggaatgcttt atgagtggca
    18241 gccaggaacg gaacttctgc cgccatgatg gtccagctta agtacgcgcc gcaaacggaa
    18301 acgatcaaac cggcagcgat gatgatttcg ccccatggtc ccatcatttc caccatcaga
    18361 ccggccattg acgggttacg aatttcagcc agttcaggac gtgccaccac acccagcgaa
    18421 agcagcgtta ccagcaagta aacgcccaga gcggagagaa ccgccagcag tgtcgctttg
    18481 ccaacatcac gtttattacg cgcacgcgca gaaacaacca cggcaccttc cacaccaatg
    18541 aaaacccaca gggtgatcag catggtgttt ttcacctgtt cccaaacagg tacgccaagt
    18601 gcaagtccgg tgaagtcgag cttgaaggta tccagtttga acatcatcat cgccagcaca
    18661 acaaacagac ccagcggcaa caattttgcc agtgtcgcca ccaggttaat gctggcagcg
    18721 gtttgcaccc cgcgcaggat caaaaagtga acaatccaca ataaagccga tgcaccgact
    18781 atcgattgcc aggtattacc atcgccaaac aggcgcaatt ccggcgtgtc cgtaaaaaag
    18841 cttaacgcgg aaaaaacgat caccagatag gagacgttgg cgatgaccgc gcacagccag
    18901 tatccccatg cggaacaaaa gccgattagc tcgccaaacc cttcgcgggc ataggtaaag
    18961 ataccgccat caagttcggg acgaatgcgc gtgaggatca gcatggcaaa ggccagcaat
    19021 aaaatgccag cgccagtaat accccagccg atgagcagtg ctgccgggct ggcaactgcc
    19081 gccatatttt gcggcagact gaaaacaccc gcgcccagca ttgagcttaa taccagcgcg
    19141 gtgagtgcgc tcagtcccag tttcttttcc atcgatatcc agtgattaaa acgtgataag
    19201 aagaataaat attagggcaa aacgcataaa aatggagttt gccactgaag cgcgggattt
    19261 tacgaagaga tgtgtctgca tgcaatggtc gcggggaaat ttgtgactaa aaatgcgaaa
    19321 ggcggcatca agccgcctta taggagtaac tgaatagtta tttatacaga tctgcgctga
    19381 cggtcaggtt attaccacgt ttctgccact ggcgggtgat gtggtaatac ttagcacctt
    19441 tcttcgcggc acgtttcgca acctgatagg agacttcggt catgttgccg tagttgccag
    19501 agaatttgat gctgtcgaac gggaccatca tcgctgctgt cgctttgttc agttcttcga
    19561 ctttagtgcc atccgggagc gtgacggtgt aacgcccgcc ttttgatgac tgggtttcaa
    19621 agaagcgacc gacttcagaa cttggtgatg cggtagtcgc aacacccggg atctcaactt
    19681 tcttcgcggc ttcgccaccg gcagccagag ctgcacgtcc tgcttcggaa tctgccggga
    19741 tcacatccgg gctctggacg atacgtttct tagcatcttt tttatagatg aatgcagtaa
    19801 tacgctggtt gccgccctgg ttggcatcga tttgacgaac gatgtagaaa gagtaggcac
    19861 ctttcgcttt tgccgctttg gtgatggcat cattgacttc tggctggcta cgatagaagc
    19921 cctggacggt gaccgtgtca aacggttcaa tcagaacagc ctgatctttc ggcagttcga
    19981 caacaccgtt aattacgcga ttacttgttt cttctgcttt ttcagcatcg gctttataga
    20041 ggtcagcgac cacacgccag ttaccgctgt taccaaaatc agaagtgtcg acaacataaa
    20101 aagaggcggc accttcttta tctgcgcgac gagaaacggc tttcaccgct tcgccaatag
    20161 cattaaaacg accggtaacc actacacggt caaaaggttt aaccgctgcc gcttgctccg
    20221 gtgtcagttc tgttgctgcg ttaacggaga atgccgtagc agaaagcagt gccgacgcca
    20281 ggagggtgtt cttaagcttc ataaaaataa tccttcgcct tgcgcaaacc aggtactggt
    20341 attgttatta acgagaaacg tggctgatta ttgcatttaa acggtgtaac tgtctgcgtc
    20401 atttttcata tcacattcct taaaccaatt ttaatcctgc tcaaatgacc gtctatgctt
    20461 aaaaaacagc cgtatcagca tcattactac tgaagcaact gaattgtata agttaattta
    20521 atgttaagta gtgattcgtg ccggggcgat gtctcgtttt acccgaccgt cgaagacaat
    20581 tatcagtctt tatccggcgt tctaaggtgt ttatcccact atcacggctg aatcgttaat
    20641 attttgcgag ttcacgccga aatactgatt tttggcgcta gatcacaggc ataattttca
    20701 gtacgttata gggcgtttgt tactaattta ttttaacgga gtaacattta gctcgtacat
    20761 gagcagcttg tgtggctcct gacacaggca aaccatcatc aataaaaccg atggaaggga
    20821 atatcatgcg aattggcata ccaagagaac ggttaaccaa tgaaacccgt gttgcagcaa
    20881 cgccaaaaac agtggaacag ctgctgaaac tgggttttac cgtcgcggta gagagcggcg
    20941 cgggtcaact ggcaagtttt gacgataaag cgtttgtgca agcgggcgct gaaattgtag
    21001 aagggaatag cgtctggcag tcagagatca ttctgaaggt caatgcgccg ttagatgatg
    21061 aaattgcgtt actgaatccc gggacaacgc tggtgagttt tatctggcct gcgcagaatc
    21121 cggaattaat gcaaaaactt gcggaacgta acgtgaccgt gatggcgatg gactctgtgc
    21181 cgcgtatctc acgcgcacaa tcgctggacg cactaagctc gatggcgaat atcgccggtt
    21241 atcgcgccat tgttgaagcg gcacatgaat ttgggcgctt ctttaccggg caaatcactg
    21301 cggccgggaa agtgccaccg gcaaaagtga tggtgattgg tgccggtgtt gcaggtttgg
    21361 ccgccattgg cgcagcaaac agtctcggcg cgattgtgcg tgcattcgac acccgcccgg
    21421 aagtgaaaga acaagttcaa agtatgggcg cggaattcct cgagctggat tttaaagagg
    21481 aagcgggcag cggcgatggc tatgccaaag tgatgtcgga cgcgttcatc aaagcggaaa
    21541 tggaactctt tgccgcccag gcaaaagagg tcgatatcat tgtcaccacc gcgcttattc
    21601 caggcaaacc agcgccgaag ctaattaccc gtgaaatggt tgactccatg aaggcgggca
    21661 gtgtgattgt cgacctggca gcccaaaacg gtggcaactg tgaatacatc gtgccgggtg
    21721 aaatcttcag tacggaaaat ggtgtcaaag tgattggtta taccgatctt ccgggccgtc
    21781 tgccgacgca atcctcacag ctttacggta ctaacctcgt taatctgctg aaactgttgt
    21841 gcaaagagaa agacggcaat atcactgttg attttgatga tgtggtgatt cgcggcgtga
    21901 ccgtgatccg tgcgggcgaa attacctggc cggcaccgcc gattcaggta tcagctcagc
    21961 cgcaggcggc acaaaaagcg gcaccggaag tgaaaactga ggaaaaatgt gcctgctcac
    22021 cgtggcgtaa atacgcgttg atggcgctgg caatcatcct gttcggttgg ctgtcaagcg
    22081 ttgcgccgaa agagtttctt gggcacttca ccgttttcgc gctggcctgc gttgtcggtt
    22141 attacgtggt gtggaatgta tcgcacgcgc tgcatacacc gttgatgtcg gttaccaacg
    22201 cgatttcagg gattattgtt gtcggagcac tgttgcagat tggccaggga ggctgggtta
    22261 gcttccttag ttttatcgcg gtgcttatag ccagcattaa tattttcggt ggcttcaccg
    22321 tgactcagcg catgctgaaa atgttccgca aaaattaagg ggtaacatat gtctggagga
    22381 ttagttacag ctgcatacat tgttgccgcg atcctgttta tcttcagtct ggccggtctt
    22441 tcgaaacatg aaacgtctcg ccagggtaac aacttcggta tcgccgggat ggcgattgcg
    22501 ttaatcgcaa ccatttttgg accggatacg ggtaatgttg gctggatctt gctggcgatg
    22561 gtcattggtg gggcaattgg tatccggctg gcgaagaaag ttgaaatgac cgaaatgcca
    22621 gaactggtgg cgatcctgca cagcttcgtg ggtctggcgg cagtgctggt tggctttaac
    22681 agttatctgc atcatgacgc gggaatggca ccgattctgg tcaatattca cctgacggaa
    22741 gtgttcctcg gtatcttcat cggggcggta acgttcacgg gctcggtggt ggcgttcggc
    22801 aaactgtgtg gcaagatttc gtctaaaccg ttgatgctgc caaaccgtca caaaatgaac
    22861 ctggcggctc tggtcgtttc cttcttgctg ctgattgtat ttgttcgcac ggacagcgtc
    22921 ggcctgcaag tgctggcatt gctgataatg accgccattg cgctggtatt cggctggcat
    22981 ttagtcgcct ccatcggtgg tgcagatatg ccggtggtgg tgtcgatgct gaactcgtac
    23041 tccggctggg cggctgcggc tgcgggcttt atgctcagca acgacctgct gattgtgacc
    23101 ggtgcgctgg tcggttcttc gggggctatc ctttcttaca ttatgtgtaa ggcgatgaac
    23161 cgttccttta tcagcgttat tgcgggtggt ttcggcaccg acggctcttc tactggcgat
    23221 gatcaggaag tgggtgagca ccgcgaaatc accgcagaag agacagcgga actgctgaaa
    23281 aactcccatt cagtgatcat tactccgggg tacggcatgg cagtcgcgca ggcgcaatat
    23341 cctgtcgctg aaattaccga gaaactccgc gctcgtggta ttaatgtgcg tttcggtatc
    23401 cacccggttg cggggcgttt gcctggacat atgaacgtat tgctggctga agcaaaagta
    23461 ccgtatgaca tcgtgctgga aatggacgag atcaacgatg actttgctga taccgatacc
    23521 gtactggtga ttggtgctaa cgatacggtt aacccggcgg cgcaggatga tccgaagagt
    23581 ccgattgctg gtatgcctgt gctggaagtg tggaaagcgc agaacgtgat tgtctttaaa
    23641 cgttcgatga acactggcta tgctggtgtg caaaacccgc tgttcttcaa ggaaaacacc
    23701 cacatgctgt ttggtgacgc caaagccagc gtggatgcaa tcctgaaagc tctgtaaccc
    23761 tgacggcctc tgcggaggcc gtcactcttt attgagatcg cttaacagaa cggcgatgct
    23821 ttgacctccc gctgtttgtt caagcgcaat tttgacaata attgtcaacg gcacggaaag
    23881 cagcataccc accggtccta acaaccatcc ccaaaaaatc aacgacaaaa ataccaccaa
    23941 tgtcgaaagc cccagcccac gtcccatgat gcgcggctcc agaatattgc cgaagaccag
    24001 attaatcagc agatatcccg ccagcaccag caacgcttcg tagaagccat taaacaccag
    24061 tacctgagcg atagggggga ttgccgcgag aactgaacca atattaggga tgtaattaag
    24121 cgcaaaggcc agcaatcccc agacaaaagc gaagcgaaca tcgagtgcgg cgagcatcgc
    24181 ccaggcgacc aggccggtga tgatgctgat ggctgttttc agcaccagat aatgagaaac
    24241 actgtcaatc gcacgttgaa tcgccgccat cccttcaacc ggacgcgcca tcatttgctg
    24301 aaattttccg ggcaattgtg gcacttcgag cagcataaac agcaccgtca gcagcaataa
    24361 aaatattgat gacatggcat tagataactg cgtcaataag ttggtgagca acgtcatcgc
    24421 cgcgttcgga tcaatgtaat gcgccagttg gtcaactgag acgtcaatcc ctacgcgttg
    24481 caacaacggc tcaagagctt gcagcggcgt cataatagag ttgcgatatt gcggtaacgt
    24541 ccgcgtcaac tcgttgagcg cggaacccag ataagctaat agcaacacca tcgccatcac
    24601 gatgattgtc atcaaaatcg acactgccag tacacgcggc acacgccagc ggaccatatg
    24661 ttgcaccagc gggttaagaa taacagcaat aaataatgcg agaataaacg gcacgatgat
    24721 ctcggcggca aaacggatac cgcagagaat aatgaccagc attcccaaca taatgacgat
    24781 ttttaggcca ttgagcgtga tgatcggctt tgccatgctt gtccccgttt ttcttccttt
    24841 gctaataata atgtcttttt cgctccgtca tcataacgaa aatcaaaccg ctcaggtaaa
    24901 gaagtgaaaa tattttgagt taattcttaa gctatgatac aaatcaggcg tgttcaacta
    24961 ccgaggacaa ttatcatctg cgatgacgag aagcaacact gcggataatt gtaatattat
    25021 ggacaatttg ttcaggacgt tattttctac atttacccat cttcgtacct catccactat
    25081 cttgctcgta ggtgagcagc actggcgcaa cgcgctttag tcctttcttc tgcctaagct
    25141 tgccctatgc gcagggcttg accactttac tttggtttcg ctgaattaag cgaaaattaa
    25201 aatagttctc ttgcaggaga aggacaatgt atatttattg gattttatta ggtctggcta
    25261 ttgctacaga aattaccggt acgctgtcaa tgaaatgggc gagcgtcagt gagggaaatg
    25321 gcggctttat tttaatgctg gtgatgattt ctctgtcgta tatatttctc tctttcgccg
    25381 ttaaaaaaat cgccttaggc gtagcttatg cgctgtggga aggtatcggt attttattta
    25441 ttaccttgtt tagcgttttg ttattcgacg aaagtttatc gctgatgaaa attgccgggt
    25501 taaccaccct ggtcgccggg atagtgttga tcaaatcagg tacccgtaaa gcgcgtaaac
    25561 ctgaactgga ggtgaaccat ggcgcagttt gaatgggttc acgccgcctg gctggcattg
    25621 gcaatcgtgc tggaaatcgt tgctaacgtc tttttgaaat tttctgacgg ctttcgtcgc
    25681 aaaatatttg gcttgctctc cctggcggcg gtgctggcgg cctttagtgc gctttctcag
    25741 gccgttaaag ggatcgactt gtctgtcgtt tatgcattgt ggggcgggtt tggtattgcc
    25801 gccacgttag ccgcaggttg gatcttgttt ggtcaacggt taaatcgtaa aggctggatt
    25861 ggcctggtct tgctgttggc tggaatgatc atggtgaaac ttgcctgatg aagacgctgc
    25921 ccgcgctgtc gggcagcgtt tgaacattat ttttgcgaca gttgatccag cttgtcgcga
    25981 aaaccggtaa cagaaatggc ccggttatcg gcgcgccagc gatctttcgc ggcaggagcc
    26041 gaactttgca ccccaattaa ttgccagccg tcatcggtat gcaacatcag aggcgaaccg
    26101 ctgtcacccg gcaaggtatc gcactgatgt gacatcaccg acgtttgcgc ccagccagtc
    26161 acttcacagt tttgatgact gtataacgta tcgagatgat cttcagggta tcccgcctga
    26221 gtcactttac gaccagccga ttttaatgcg gcagtaagcg cggctttatc tccctcaaat
    26281 aacggcaacg gtgtaatgcc agaaggggga ttacgtagca caatcaatcc gaagtcccac
    26341 ggtgcagctg cgggaggaac aatccaacca tccccatctg cttttaaccg ctttcccagt
    26401 gtcggatcaa cgcggccttc tatgtcgtgg atctcatagc gccaaagacc tttatttgac
    26461 acaaaacgca gcgctaccgc cttatccgct ttacccttag gcggcgtcaa taaacagtgt
    26521 cctgccgtta atgccagatt gggtgcaatc agcgtcgccg tacataaatt gccgctggcc
    26581 gtttccagtt gcccaaccgc atcccacggt gattgggtcg tgtcattcac tggcacacga
    26641 tcatcatgac caaaaaacag ggtgctgacc tcatcgtttg ccgatttggc aacgtctggt
    26701 ttatctgcaa acacaaaagc agacgtcaaa ctaattgcac ccaacactac agcaatggtt
    26761 gtacgcatat cacactctgg tgggtaatta tgattattaa aagcaaaccc tcataaatac
    26821 tatagacggg acgggatgaa agtgggagta aaatcagata actacagtca ggaaagataa
    26881 aaacgggcgg caatcagtgc gcataaaata aggatgataa gaatgaactc aaagcgataa
    26941 cggcccacca tactaccctc cgctaaaggc ggtgctaaac gttacccgct tagcaccgta
    27001 attttcaggc gcgagggggc gcgccagcat tactgttgaa aacttacgct gcgggttgtg
    27061 cagcaggttt tgccggttgc tgatggccgt gtttcccggc gtgtttcttc gctgcctgcg
    27121 ctttttgttc aggggctttc tgttcagctt tcgtattttt atgatgcttt ttagccgcct
    27181 gcgctttctg ggcaggtgct gctttatgct gttttttatg atgtgtagtt ttcgctggcg
    27241 ctgctttggt ggtcgtcgca gtcggagcag gtgtggtcgc agtctctgca gcaaaggcgg
    27301 cagaagacag acccatagca gcggcaacaa ccagagctaa tacttttttc attgtcatac
    27361 cctcaatttg ttttttcatt taaccccact gcggggccgt tgaaataact atatccctgg
    27421 gaatggcaga cttccgtgag tggttggttt cagcgtgtaa cgatatgtac aaacgctgaa
    27481 taaattacag cgttgataat aacgtattgt gacttaagga aaatttagct accaataata
    27541 gtagtcttga tcgggttaac tattatacta cttacaaagc ggtagaataa ctgcgcatca
    27601 gtaataaatg acacacagca aaatgaatcc gtttatttgg gtacttataa tcctgatgac
    27661 gctagatgcg ctgcgggaat tggctggcgc ttcgtctatt ttaggatggc tattaacgtt
    27721 ggtttgagca ggcaataagt ccggacgggt atttaccgca gtccggactt atttttcagg
    27781 cgtgcagacg acgatgcaaa cgcgtcccga ccagcagagc aatgaccagc atcagagcaa
    27841 taaatgcgcc gacgccgttc cagccatagt tatgccagaa aacaccaccc agcgtcccgg
    27901 caatactcga ccccagatag taactgaaca gatacagcga ggaggcctgg cctttagcgc
    27961 gttttgcgcg ggggccgatc cagctgctgg ctactgagtg ggctgcgaag aatccggctg
    28021 agaagagtaa cattccggca aagatcagcc acagcgagct aaataaggtc atcagtaaac
    28081 caaacggcat aactcccgtc gaaaacaaca tcaccggacc gcgcccatag cgggtggtca
    28141 tggttccggc tttgggtgag ctccatgtac cggtcaaata agccagcgat aataagccaa
    28201 ccacggcctg actgacatgc tagggggaaa gcatcaaccg atagccgatg taattaaaca
    28261 gcgtgacgaa cgaccccatc agcaaaaagc cttctgcgaa caataacggt aatccccggt
    28321 cacgccagtg cagacgaaag ttgataaata acgtcttagg gcgcagcgaa gtcgggcaaa
    28381 aatggcgtga ttcagggagg attttccaga acatcaacgc cgaggccagc gcgaaacaac
    28441 cgattgccgc cagagcaatt cgccagttga aaaagtccgt gaagacacca ctaattaagc
    28501 gtccgctcat gccgccaatt gagttgccgc tgatatacaa ccccatcgaa aaggccacga
    28561 aactggggtg gatttcctcg ctaagataag tcatgccaac agctgccacg ccacttaacg
    28621 aaagcccaat caaggcgcgc ataatcaaaa tgccgtgcca gctggtcatc attgtcgaaa
    28681 gtaacgtaca aatggaggcc aacagtagcg ccgtgaccat cactggtttg cgaccaatgg
    28741 catcggatag cgggccagta aatagcaaac cgatagccaa catcgccgtg gaaatggaca
    28801 gtgaaatact actgttcgcg ggcgttaagc caaactcctg cgaaagcacc ggaaggatag
    28861 gctgcacaca atagagaagt gcaaaggttg ccagtccggc agagaacagc gccagggtga
    28921 cgcgcataaa ttgcggcgta ccgcgtttaa taaattgatt tggctgagaa atgctttgct
    28981 tgtcagtgtc gcttgccgga gcgccatcaa cagttgtagt acggctcact tgaaatcctt
    29041 gctaaatatg cctgtggatc aggcttatac atagggtagg aaaatcgaat tgttctgtct
    29101 aatatattaa taatctcaaa taagatgttt taaatatgaa tattgaactt cgtcatctgc
    29161 gttactttgt tgctgttgcg gaagagctgc attttgggcg cgcggctgcc cgcctgaata
    29221 tttcgcaacc gccgctaagt cagcagattc aggcactgga gcaacaaatc ggtgcccgac
    29281 tgctggcacg aactaatcgc agtgtattgc tgacggcagc aggaaaacag tttcttgcag
    29341 atagtcggca aatcctgtct atggtggatg acgctgccgc tcgcgctgaa agactgcatc
    29401 agggtgaagc gggggagttg cgtattggtt ttacttcgtc cgctcctttt attcgggcgg
    29461 tgtccgatac gttatcgctg tttcgccgtg attatcctga tgtccattta caaacccgcg
    29521 aaatgaacac tcgcgagcaa atcgctccgc tcattgaagg aacgctggat atgggattgc
    29581 tgcgtaacac agcgttaccg gagacgcttg aacacgcagt catcgtccat gaaccgctta
    29641 tggcgatgat cccgtacgat catcccctgg caaataatcc gagtgtaaca ctggctgaac
    29701 tggctgaact ggcgaaagaa ccctttgtct tttttgatcc gcacgtcggg actgggctgt
    29761 atgacgatat tctcggactg atgcgacgtt accatttgac gcccgtcatc actcaggagg
    29821 tgggcgaggc aatgaccatc atcggtctgg tttccgccgg tctgggtgtt tcaattttgc
    29881 ctgcgtcatt taaacgtgtt cagctcaacg aaatgcgctg ggtgccgatt gctgaagagg
    29941 atgcggtttc tgaaatgtgg ttggtctggc cgaaacatca tgaacaaagt ccagctgcgc
    30001 gtaactttcg tattcatctg ctgaatgctc tcaggtgagg gaaatttcag cgaaaaagcc
    30061 cgaaaaatgt gctgttaatc acatgcctaa gtaaaaattt gacgacacgt attgaagtgc
    30121 ttcaccatag cctacagatt atttcggagc gcgaaaatat agggagtatg cggtggttgc
    30181 tgaaaaccag cctgggcaca ttgatcaaat aaagcagacc aacgcgggcg cggtttatcg
    30241 cctgattgat cagcttggtc cagtctcgcg tatcgatctt tcccgtctgg cgcagctggc
    30301 tcctgccagt atcactaaaa ttgtccgtga gatgctcgaa gcacacctgg tgcgagagct
    30361 ggaaatcaaa gaagcgggga accgtggccg tccggcggtg gggctggtgg ttgaaactga
    30421 agcctggcac tatctttctc tgcgcattag tcgcggggag attttccttg ctctgcgcga
    30481 tctgagcagc aaactggtgg tggaagagtc gcaggaactg gcgttaaaag atgacttgcc
    30541 attgctggat cgtatcattt cccatatcga tcagtttttt atccgccacc agaaaaaact
    30601 tgagcgtcta acttcgattg ccataaccct gccgggaatt attgatacgg aaaatggtat
    30661 tgtacatcgc atgccgttct acgaggatgt aaaagagatg ccgctcggcg aggcgctgga
    30721 gcagcatacc ggcgtaccgg tttatattca gcatgatatc agcgcatgga cgatggcaga
    30781 ggccttgttt ggtgcctcac gcggggcgcg cgatgtgatt caggtggtta tcgatcacaa
    30841 cgtgggggtg ggcgtcatta ccgatggtca tctgctacac gcaggcagca gtagtctcgt
    30901 ggaaataggc cacacacagg tcgacccata tggtaaacgc tgttattgcg ggaaccacgg
    30961 ctgcctcgaa accatcgcca gtgtggacag tattcttgag ctggcacagc tgcgtctcaa
    31021 tcaatccatg agctcgatgt tacatgggca accgttaacc gtggactcat tgtgtcaggc
    31081 ggcattgcgc ggcgatctac tggcaaaaga catcattatc ggggtgggcg cgcatgtagg
    31141 gcgcattctt gccatcatgg tgaatttatt taacccacaa aaaatactga ttggctcacc
    31201 gttaagtaaa gcggcagata tcctcttccc ggtcatctca gacagcatcc gtcagcaggc
    31261 ccttcctgcg tatagtcagc acattagcgt tgagagtact cagttttcta accagggcac
    31321 gatggcaggc gctgcactgg taaaagacgc gatgtataac ggttctttgt tgattcgtct
    31381 gttgcagggt taacattttt taactgttct accaaaattt gcgctatctc aatttgggcc
    31441 aggaaagcat aacttagact ttcaaggtta attattttcc tagtttatat ttgtgaagca
    31501 taacggtgga gttagtgatg ctgaagcgtt tctttattac cggtacagac acttctgtag
    31561 ggaaaacggt ggtttcccgc gcattgctac aagcgttagc ctcccaggga aaaagggttg
    31621 cgggatataa acccgtagcg aaggggagca aagagacacc cgaagggctg cgtaataaag
    31681 atgccttggt gttgcagagt gtttcaacca tcgaactgcc ttatgaagca gttaatccta
    31741 tcgcgttaag cgaagaagaa agtagcgtgg cgcacagttg cccaatcaat tacaccctca
    31801 tttcaaacgg cctggcaaac ctgaccgata aagtcgatca tgtcgtggta gaagggactg
    31861 gcggctggcg cagtctgatg aatgatttgc gtccactctc tgaatgggta gtgcaggaac
    31921 aactgccggt gttgatggtt gtcggtattc aggaaggttg cattaaccat gcactgctaa
    31981 cagctcaggc gatcgccaac gacgggctgc cgctcattgg ctgggtggct aaccgaatca
    32041 acccaggact ggcgcattat gcggaaatca ttgatgtgct gggtaaaaaa cttccggcac
    32101 cgctcattgg tgaactgcct tatctgccgc gcgccgaaca gcgtgaactg gggcaataca
    32161 tccgcttagc tatgctgcgc agtgtgctgg cggtagatag agtcacggtg taacgtccgc
    32221 gaaattaccg acgcaattac gcaggcaatc aataaaccgg ggagtagctg atactccccg
    32281 gtcatttcac atatcatcaa cgtcgacata atcggcgcgt gcgtggttgc cgccaacagt
    32341 gtcgccatcc cggtcaatcc gagtaaaagc gtaatttctt cgccatcagg gaaccataat
    32401 cccaggctac gaccatacaa catgccaatg gcaagaccga taaatagcgt cggtgtaaag
    32461 acaccaccgg gtgcgccgga accgctactc gccagcacgg cacacagttt acaaaggaag
    32521 atcccggcaa tgatcattaa cagtggtggg gcggttaaaa aggattgtac ggtgctatag
    32581 ccgttgcccc acactgcagg tgtgaacagg gaaagcagac ccacgatcaa cccgcctagt
    32641 gccagttgcc agggcggcgc aagtttgaga ctcacaaatc cacgatgaca ggcgttcatt
    32701 aacgttaaca acagtggtcc gcacagacct gccagcacac ctgtactgat aatcagcgca
    32761 tagtcacgag cctgaaccgt cactgagagt tgtacgttgt agagtaacgc gtcgctatga
    32821 ttaatcagat tgctaaccag ccatgccacg atggcggaaa taatcaccgg gccgagagag
    32881 gccaacatca tagtgccaaa cagcacttcg gctataaata aactgccagc aagcggggca
    32941 cgataggccg cagccattcc cgccgcggcc ccacaggcga tccataattt ccattcctgg
    33001 cgtggcgtaa aacgttgggc aaaacaggag gcggcaaggg cagctaaaag aatcatcgca
    33061 ccttcgcgac caattgcact gccgctggtt actaccagca gcgaggcaag cgatttaacc
    33121 aggcttgctg cgtaatcgaa ctgtccatcg gtttgcaacg cttccatgta atcggtcggc
    33181 gcatgagggc gttgttgggt aaatttctgc cagcccatca gcaacaaacc cgccgccagt
    33241 ccgccgagcg ccggagttag cagccgtcgc caggaggaaa ggtttgtcgc tgcattgacc
    33301 agactgccgg agtcattatt gaggaacaac cactccagta gcagcatcgc atgacgaaac
    33361 ccggcaacgg caaagacagc gagaataccg acgactgttg cgataagcag acggcggaac
    33421 atagtgcgca gatccggata gatgtgtaga tgatgcatga gcagaaatgg gcaatgaaac
    33481 acgggcgact attttgcggc aaaagaggcc aaccgcaaaa gcagtgcgtg ctttaagaga
    33541 taaaaaaacg tgacattgta acgtattatt gccgaatcgt gcgtaatcgt caccatccgg
    33601 caatattacg gtgatcctat cgaaagagtg gtttaaccgc gacaggaatc aacagttgtg
    33661 attgccattg cgccagcgtt aagcgagcca gttcacccag tgcacggtag aaagggtgtt
    33721 ctgctttttc gataaaaaca tcaagaaaac gtgttgacca cggaaaaagg tgccatgcca
    33781 gcaattcttc gcattctgtc tggcgaccat tctccgccaa ccacgcagcc atcaacagca
    33841 gtgaaccaaa atgatcttcc ggttcgtttt gcttcatttc aaactgaatg cctttctcgc
    33901 gcatccactg acgaagtgcc aatgttgaat cgccaaacag cacagattcg cgatccagcc
    33961 agaccgaccc ccacggcgga gacggcagtg cccacgggcc gacaaacaaa cgctgccagg
    34021 cctgggcgtg agtctcttca cactgtgtct gaaaagccgt caccagcggc gctaatgacg
    34081 cttctggtag aggccactga gtttcccagc cgtcactggt gagtaccgca accagaggtg
    34141 cggcttccgc actctctgga gcgtaataaa acagcgcgcc caacacgcgc gccgcgacag
    34201 aaaaattatc ttgctgtgaa aaatgggtca ttcctaacat cctgaaatgt gcgggttgcc
    34261 ccgcaccaat ttgttaacct gcaatagcca ttcctactgt catgtgcagg ccataaaaga
    34321 gcacacgccc gatcatctca ccaacaagta tgaggattag ccccagaaca agtccggcaa
    34381 cgtgaggttc acggcgacga atgagcggac aaagccagca tccaagacct gcgcataaca
    34441 ataccacacg ccagacctgt aaggaggcgt aatctggtac cagtgcgctg gcttgttgca
    34501 cggatgagtg aatagacgcc agagacaaac cttgcagcac aatcacctcg gcacaagcga
    34561 tcagcgccag aacgctaatg atggcaaatg gtgtggtatt aaatgtaacg cgggctgccc
    34621 gcagaattgc ggcagcaagt attggaccgc tcaacaatac cgtcaggaag aaagccagcg
    34681 tagtgtaacc gttatgccag gttggcacgg tgtcgatttg atacacgcag gtcatcatcc
    34741 agacgaaaat gacgccgagc gccatgctga caagtaacca gagtttcccc aacgcttgcg
    34801 gcattttacc gatgaccgcc accagccacc atagtccgcc aacggcaaaa aatatggaac
    34861 cggcagcaat ctcgttactc agaccagaag ctccgattcg attcagcgag ttaaacgctc
    34921 gcagtggcga tcccaggtgc atgacagagg caataaaccc gatgcccatc aacagccaga
    34981 ggaaaaacat gccacgaaca atgtgctgac ggtctgcgtc atttttggcg gcgaaccagc
    35041 caattccgct aacaatcagc gcgcctacta cgcattgacc caatacggta aagataacca
    35101 gtggccattc atgccatcca tttcccattt tacacttcct ccggattagc cagataacca
    35161 gtggtatcgc cggtcgggcg gctgttggcg ttaggtttga taacgatatt gggttttgtg
    35221 aaatgcgcgc gcggcagtgg cgcgacggca gccagagtgc cgtgcttctg gcgcagctct
    35281 tcaattggac cgaactcaag tgcacgcagc gggcaggatt cgacgcatat gggttgtttc
    35341 ccctcggcga cgcgcgaata acaaccatcg cacttcgtca tgtgcccttt ttcagcattg
    35401 tactgtggtg cgccgtacgg acaagccatg tggcagtagc ggcagccgat acaaacatct
    35461 tcatcaacca ccacaaagcc atcttcacgc ttatgcattg cgccgctggg gcagactttt
    35521 gtacatgcgg ggtcatcgca atggttacag gagatggaga gataataagc gaacacgttc
    35581 tggtgccaga tgccattatc ttcctgccag tcgccgccag catattcata aatacggcgg
    35641 aaactgactt ccgggccaag atctttaaaa tctttgcacg ccagttcgca ggttttacag
    35701 ccagtacagc ggctggaatc gataaaaaat ccatattgtg tggtcatggg ctactcctta
    35761 aaccttttcg atctggacaa gattgctgtg cgacgggttt ccctttgcca gcggtgaagg
    35821 gcggtgagag gtcagaatat tgatactgcc gccgtgatcg acccggtcac caaacatatc
    35881 cgctttaagc cacgcacctt gcccgatggc ggtaacgcca ggcagaatac gcggagtcac
    35941 ttttgcggca atcagcattt ctccattatt gttaaatacc cgcacggtat cgccatgacg
    36001 gataccgcgt gcctgagcat caatggggtt gatccacacc tcttgtgggc aggcctgctg
    36061 taacacatca atattgccgt agctggagtg ggtacgcgct ttggtttggc gacttcactc
    36121 agcatccagt agatgggttt gcgttcaaat tttgctgagg ttgcgggttg ggcgaggatc
    36181 acatagccca tattccctgc agattcatga gagataaggt cttcttgctc tgttggcatc
    36241 aggtcaggca acaggatatc gcaatactta gccgaggccg tcatgaagtg gtcaatgcca
    36301 acaatcatct cgcacttgct gtcatcctga agcacctcat gggtgtgatt gatgtcgcca
    36361 tgttgattga tcaatgtgtt actggcgtag caccataaaa acttgatggg gacatccagt
    36421 ttttcttttc cacgaacacc atcacgggtc gcggtcattt tcgtaccatg gtcgatggca
    36481 tctgtccatg taaaaacgga aatctgcgtt ttaacaggat tctcgagcat cgggaaccat
    36541 tctaccccca gatcccagct accttcgcgt acacctgagt tgccgccgtt tatgccgacg
    36601 ttaccggtaa gaacggaaag catggcaata gcgcgggacg tttgttcgcc gttggagtgt
    36661 cgttgtggcc cccaaccctg acaaatataa gcaggttttg ctgaaccgat ctctcgtgcc
    36721 aactggataa ttttttctgc cgggatgctg gtgatttttg ctgcccattc cggcgtttta
    36781 gctatgccgt caggcccttc gcccagaata taggctttat aatgcgcgtt acgtggtgcg
    36841 ttggcgggca gcgttttttc atcgtaacca acacaatatt tgtcgagaaa tggctgatcg
    36901 accatgtttt cagtaatcag tacccaggca atcgcacagg ccagtgcgcc atcggtgcca
    36961 gggcgaatgg gcagccattc atcttcacgc ccggcagctg tgtcgttata gcgtggatcg
    37021 atgacgatca tgcgtgcgtt tgaacgttcg cgggcttgct cgacgtagta agtgacacca
    37081 ccgccgctca tccgcgtttc tgccgggtta tttccgaaca taacgaccag tttcgtattg
    37141 gcgatatcat ccgggctgtt gccatcattg gcaccgaaca tataactcat tgcggcactg
    37201 atctgtgcgg tactgtagct gccatagcga ctgagaaaac ccccgcaaga gttcatcaga
    37261 cggtacggga cgttcgagtt ggtgatattt ccgccatcta cgcctgttcc gtacaggaca
    37321 tgtacagcct cattgccgta atctttcagg atccgccgaa gattatcact gatggtatcc
    37381 agggcttcgt cccaacttat ccgttcaaat ttaccttcac cgcgctcgcc gacgcgcttc
    37441 atgggatatt tcaacctgtc aggatgattc atccgtcggc ggatagagcg cccgcgtaaa
    37501 catgcccgaa cctgatgatt accgtaaacg tcgtcacctg tcgtatcaga ctccacccag
    37561 tacacggtgt catctttcac atgcaaacgt agcagacagc ggctcccgca gttaacggtg
    37621 caggaactcc agaccgtttt ctcttctacc ggagcctctg ccgcccggac catttgggaa
    37681 aatggcagag tgaaagcact gcttgccagc gcgagactgc caagtgcgga ggttttcatc
    37741 agacttctat ggctgatttc agccttcatg agcgcctctg tggtatggat tttcatcatt
    37801 actcacttat tgcttttcaa acaaaatgtc atgccagaat ttatggttgt agtgggttat
    37861 attttttcga tctcgaccag attagtgtgc tgcgggtttc ccttcgccag tggtgaaggg
    37921 cgcagagtgg ttagcgtatt cacacatccg ccatggtcga ttttatcgcc agacatattg
    37981 ggctcgtgcc aggctccctg gcccatagcg ctaactccag ggagaatgcg tggtgttact
    38041 ttggctggta gccgaacttc gccacgatgg ttaaacaccc gcaccatatc gccgttggca
    38101 atcccacgtt tctgtgcatc tatagggttg atccacacct cctgacggca ggctgccttc
    38161 aggacatcaa tgttgccgta ggtcgagtga gtacgggatt tgtaatggaa accaaacagt
    38221 tgcagtggga aggttctacg ttcaggggag tcccagcctt caaaggttga ggcataaact
    38281 ggcagtgggc ttatcacttc atctttttcc agttcccagg tacgggcaat ttccgccagc
    38341 ttgctggaat aaatttcaat cttaccggaa ggcgttttaa gtggatttgc ctcggggtcg
    38401 tcacgaaatg ctttgtaggc gacaaaatgg ccattgggat ctttacgctt atagataccc
    38461 atttttttca gttcgtcgta agacggtaac gccggatctt tggcaagcat tttggcgtac
    38521 agatgttgta accattgttc ctgcgtgcga ccttctgtga acttttgata gacgtcaggt
    38581 ccaagacgtt tcgcgacttc actcaggatc cagtaaatcg gtttacgttc gaatttttcg
    38641 ctggtgactg gctggaggaa aatgagatat cccatgttac cggcgtagtc gttaggaata
    38701 atatcttcct gctcaacggt catcaggtct tccagcagaa tgtcggcata ttttgccgat
    38761 gaggtcataa agttttcgat gaccacaatc atttcgcatt tcgattcgtc ctgcagaatt
    38821 tcatgcgttt tgttgatgtc agaatgctga ttaacgaggg tatttcccgc gtagttccag
    38881 atggactcca ttgccagacg gatggatgga cttgaagtag ttgactcaat gctacttaag
    38941 gtcctgatat gactgacatt aatcatgata acactgtata tccatccagt tctttctctg
    39001 ggtctcccta tcggtatcag caagtctaca cctcaagccc tgacacaagg ctgacacaaa
    39061 tcaatctact cagcgtagct aagcctcagt tagtccgcag agccaatggt cgatatacta
    39121 tccgcttcag actcaaggga cagaccacac cgtttctttc agtatcgacc cgcagcacag
    39181 acaggagagt agccacaatg cgtcagagag agcttgcagc caccgctaag gccttcatgt
    39241 tggataggcc tgaagtctca ctacaggagc ttacagagca tctccggtct atggctgagc
    39301 agttcctgac agacgctagt gacgattact ggaatggact tgaggtcgct actctggtcg
    39361 acgagaagtc caacctgaag gagctagccg ctacgcaagc cttgagtctg gaccagcaga
    39421 agggcataag gttagcgctg gaagtcctga ccgcagcaca gcagagagtc gacactggtg
    39481 atacctctgg tctgattaag ctgattgatg ataataacct tactgatgac tcaactatag
    39541 gcgactctac gtcaattctg aataatgagc agggggatag accggcagtt ttcacacagg
    39601 agcgccagtc atctgtagtt ttttcaagcc tagtgtcctc gctgctggct gagaaggtcc
    39661 agaccctgaa gacatccagc tacaaggacc tctcgtcctc actcaatact gtctcccggt
    39721 tcctgcctga agacatggac ctgatgtccc gctctgggtg gctggctgtc agggactcta
    39781 tgctggcttc tgaggtgaga ccatcgacaa tcaacaagct gctgaccaaa gcgaagatgt
    39841 gtctcgatta tggcctgatg aatgggcaac ttgagggccg caaccctatt gaacggatga
    39901 agctgaccaa ggatatcgac tccaagcgca gagcgttcac agacgaggag ctggagagac
    39961 ttctggtccg tgttgagt
//
LOCUS       DAZPCS010000022        39580 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZPCS010000022 DAZPCS010000000
VERSION     DAZPCS010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39580
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.022"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..529
                     /locus_tag="ACHUQ7_001114"
     CDS             <1..529
                     /locus_tag="ACHUQ7_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1134624.1"
                     /translation="SEVGLTKYAQHLASFAEEFYIKEQKSHSESEMRGLFSSLAELYS
                     FGNDTASANRVFHQHVPAFNTDHMIQYFINAKEWNNARELLIKEEMLGMHNLILLENI
                     CMQKNAECMAHITFTLNKLTTQPAITKIDSVGNEQLYQIGKIYHKLEIKPEPEQQVLI
                     QSLYDRASGSASATQ"
     gene            complement(589..1080)
                     /gene="yajQ"
                     /locus_tag="ACHUQ7_001115"
     CDS             complement(589..1080)
                     /gene="yajQ"
                     /locus_tag="ACHUQ7_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFR1134625.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            1248..2159
                     /gene="panE"
                     /locus_tag="ACHUQ7_001116"
     CDS             1248..2159
                     /gene="panE"
                     /locus_tag="ACHUQ7_001116"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFR1134626.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            2122..2712
                     /gene="yajL"
                     /locus_tag="ACHUQ7_001117"
     CDS             2122..2712
                     /gene="yajL"
                     /locus_tag="ACHUQ7_001117"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFR1134627.1"
                     /translation="MSASALVCLAPGSEETEAVTTINLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(2766..4214)
                     /gene="thiI"
                     /locus_tag="ACHUQ7_001118"
     CDS             complement(2766..4214)
                     /gene="thiI"
                     /locus_tag="ACHUQ7_001118"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFR1134628.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            4420..4662
                     /gene="xseB"
                     /locus_tag="ACHUQ7_001119"
     CDS             4420..4662
                     /gene="xseB"
                     /locus_tag="ACHUQ7_001119"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFR1134629.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            4662..5561
                     /gene="ispA"
                     /locus_tag="ACHUQ7_001120"
     CDS             4662..5561
                     /gene="ispA"
                     /locus_tag="ACHUQ7_001120"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFR1134630.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQALKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            5586..7448
                     /gene="dxs"
                     /locus_tag="ACHUQ7_001121"
     CDS             5586..7448
                     /gene="dxs"
                     /locus_tag="ACHUQ7_001121"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFR1134631.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPETAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            7503..8420
                     /gene="yajO"
                     /locus_tag="ACHUQ7_001122"
     CDS             7503..8420
                     /gene="yajO"
                     /locus_tag="ACHUQ7_001122"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFR1134632.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKP"
     gene            complement(8531..9049)
                     /gene="pgpA"
                     /locus_tag="ACHUQ7_001123"
     CDS             complement(8531..9049)
                     /gene="pgpA"
                     /locus_tag="ACHUQ7_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFR1134633.1"
                     /translation="MTILSRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGSMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(9027..10004)
                     /gene="thiL"
                     /locus_tag="ACHUQ7_001124"
     CDS             complement(9027..10004)
                     /gene="thiL"
                     /locus_tag="ACHUQ7_001124"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFR1134634.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFVTP"
     gene            complement(10082..10501)
                     /gene="nusB"
                     /locus_tag="ACHUQ7_001125"
     CDS             complement(10082..10501)
                     /gene="nusB"
                     /locus_tag="ACHUQ7_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFR1134635.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(10521..10991)
                     /gene="ribE"
                     /locus_tag="ACHUQ7_001126"
     CDS             complement(10521..10991)
                     /gene="ribE"
                     /locus_tag="ACHUQ7_001126"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFR1134636.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(11080..12183)
                     /gene="ribD"
                     /locus_tag="ACHUQ7_001127"
     CDS             complement(11080..12183)
                     /gene="ribD"
                     /locus_tag="ACHUQ7_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFR1134637.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGVLLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(12187..12636)
                     /gene="nrdR"
                     /locus_tag="ACHUQ7_001128"
     CDS             complement(12187..12636)
                     /gene="nrdR"
                     /locus_tag="ACHUQ7_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFR1134638.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            12787..13326
                     /locus_tag="ACHUQ7_001129"
     CDS             12787..13326
                     /locus_tag="ACHUQ7_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFR1134639.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            13624..14508
                     /gene="tsx"
                     /locus_tag="ACHUQ7_001130"
     CDS             13624..14508
                     /gene="tsx"
                     /locus_tag="ACHUQ7_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFR1134640.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(14685..15032)
                     /gene="yajD"
                     /locus_tag="ACHUQ7_001131"
     CDS             complement(14685..15032)
                     /gene="yajD"
                     /locus_tag="ACHUQ7_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFR1134641.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(15161..16132)
                     /gene="secF"
                     /locus_tag="ACHUQ7_001132"
     CDS             complement(15161..16132)
                     /gene="secF"
                     /locus_tag="ACHUQ7_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFR1134642.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALTHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(16143..17990)
                     /gene="secD"
                     /locus_tag="ACHUQ7_001133"
     CDS             complement(16143..17990)
                     /gene="secD"
                     /locus_tag="ACHUQ7_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFR1134643.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(18018..18350)
                     /gene="yajC"
                     /locus_tag="ACHUQ7_001134"
     CDS             complement(18018..18350)
                     /gene="yajC"
                     /locus_tag="ACHUQ7_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFR1134644.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(18373..19500)
                     /gene="tgt"
                     /locus_tag="ACHUQ7_001135"
     CDS             complement(18373..19500)
                     /gene="tgt"
                     /locus_tag="ACHUQ7_001135"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFR1134645.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLLEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(19555..20625)
                     /gene="queA"
                     /locus_tag="ACHUQ7_001136"
     CDS             complement(19555..20625)
                     /gene="queA"
                     /locus_tag="ACHUQ7_001136"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFR1134646.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            20718..21291
                     /gene="acpH"
                     /locus_tag="ACHUQ7_001137"
                     /pseudo
     CDS             20718..21291
                     /gene="acpH"
                     /locus_tag="ACHUQ7_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
     gene            complement(21296..23113)
                     /gene="malZ"
                     /locus_tag="ACHUQ7_001138"
     CDS             complement(21296..23113)
                     /gene="malZ"
                     /locus_tag="ACHUQ7_001138"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFR1134647.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNLGTGGACHNSES
                     PWRDWYSFSDDGTALDWLGYASLTKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYRRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEASEV
                     VLPASPFLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(23269..24642)
                     /gene="proY"
                     /locus_tag="ACHUQ7_001139"
     CDS             complement(23269..24642)
                     /gene="proY"
                     /locus_tag="ACHUQ7_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFR1134648.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(24718..26037)
                     /gene="brnQ"
                     /locus_tag="ACHUQ7_001140"
     CDS             complement(24718..26037)
                     /gene="brnQ"
                     /locus_tag="ACHUQ7_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFR1134649.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVVAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(26444..27739)
                     /gene="phoR"
                     /locus_tag="ACHUQ7_001141"
     CDS             complement(26444..27739)
                     /gene="phoR"
                     /locus_tag="ACHUQ7_001141"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFR1134650.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(27797..28486)
                     /gene="phoB"
                     /locus_tag="ACHUQ7_001142"
     CDS             complement(27797..28486)
                     /gene="phoB"
                     /locus_tag="ACHUQ7_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFR1134651.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIKMQGLSLNPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            28676..29878
                     /gene="sbcD"
                     /locus_tag="ACHUQ7_001143"
     CDS             28676..29878
                     /gene="sbcD"
                     /locus_tag="ACHUQ7_001143"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFR1134652.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGSSKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEDLDESQQQRLQHLFTTTLR
                     TLAGEHEA"
     gene            29875..33018
                     /gene="sbcC"
                     /locus_tag="ACHUQ7_001144"
     CDS             29875..33018
                     /gene="sbcC"
                     /locus_tag="ACHUQ7_001144"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFR1134653.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHVRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            33144..34325
                     /gene="araJ"
                     /locus_tag="ACHUQ7_001145"
     CDS             33144..34325
                     /gene="araJ"
                     /locus_tag="ACHUQ7_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFR1134654.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMSVLTELAHNVGISIPAAGHMI
                     SYYALVVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQECWRYTFL
                     LIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAWF
                     SYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIVL
                     ALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFNL
                     GSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKPL
                     G"
     gene            complement(34468..35376)
                     /gene="mak"
                     /locus_tag="ACHUQ7_001146"
     CDS             complement(34468..35376)
                     /gene="mak"
                     /locus_tag="ACHUQ7_001146"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFR1134655.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNVNSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVSKAKHGDSSGVRGAAWLWPQE"
     gene            35501..36412
                     /gene="rdgC"
                     /locus_tag="ACHUQ7_001147"
     CDS             35501..36412
                     /gene="rdgC"
                     /locus_tag="ACHUQ7_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFR1134656.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(36490..36945)
                     /locus_tag="ACHUQ7_001148"
     CDS             complement(36490..36945)
                     /locus_tag="ACHUQ7_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1134657.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(36942..37277)
                     /locus_tag="ACHUQ7_001149"
                     /pseudo
     CDS             complement(36942..37277)
                     /locus_tag="ACHUQ7_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(37432..37716)
                     /gene="ppnP"
                     /locus_tag="ACHUQ7_001150"
     CDS             complement(37432..37716)
                     /gene="ppnP"
                     /locus_tag="ACHUQ7_001150"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFR1134658.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(37788..38465)
                     /locus_tag="ACHUQ7_001151"
     CDS             complement(37788..38465)
                     /locus_tag="ACHUQ7_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFR1134659.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(38723..38914)
                     /gene="yaiA"
                     /locus_tag="ACHUQ7_001152"
     CDS             complement(38723..38914)
                     /gene="yaiA"
                     /locus_tag="ACHUQ7_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFR1134660.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(38964..39488)
                     /gene="aroL"
                     /locus_tag="ACHUQ7_001153"
     CDS             complement(38964..39488)
                     /gene="aroL"
                     /locus_tag="ACHUQ7_001153"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFR1134661.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAASTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
BASE COUNT         9946 a        10569 c         9894 g         9171 t
ORIGIN      
        1 tagtgaagtg ggtctaacca aatatgctca acatctcgca tctttcgctg aagaatttta
       61 tataaaagag caaaaatcac attctgaaag tgaaatgaga ggactatttt catcacttgc
      121 cgaattatat agttttggta atgatactgc cagcgcaaac cgagtgttcc atcagcatgt
      181 ccctgccttc aatacagatc atatgattca atattttata aatgccaaag aatggaacaa
      241 tgccagggaa ttattgataa aagaggaaat gttgggtatg cataatctga tattgttgga
      301 gaatatttgt atgcaaaaaa atgcagaatg catggcacac attaccttta cgcttaataa
      361 attaacaaca caaccagcaa taacaaaaat agatagcgtg ggtaatgagc aactttatca
      421 gattggcaaa atctaccata agttggaaat taaacctgag ccagagcaac aggttttaat
      481 tcagtcgctt tatgaccggg catcaggaag cgcctctgca actcaataaa tgcatggtca
      541 aaagggccac cacggtggcc ctgacaatca gtagctatat ccagaaagtt aatcgcggaa
      601 gtttttgaac tggaacggct gaccgagatc gccaccgcgc accatcgcca tgacagcctg
      661 caaatcatca cgagatttgc cagtcacgcg gatctcatcg ccctgaattt gcgcctgcac
      721 tttcagtttg ctgtctttga tcatcttgac gatttttttc tgagtggcgc tctcaatgcc
      781 ctgtttcagt ttcgcttcca caaaccaggt tttaccgcta tgaacgatat tttccggtac
      841 atccagtgaa ctgccttcaa tgccgcgctt cagcagcttg gcacgcagaa tatccagcaa
      901 ctgattgacc tggaagtcgg actcgctcaa cactttgatg gttttgctgg cgtcgttcag
      961 ctcaaatgag gcttcaacgt tacggaagtc aaaacgggac tccacttcgc gactcgcgtt
     1021 atcgaccgcg ttacgtgctt cctgaagatc aacttcagag acaatatcga aagatggcat
     1081 cttttcctct cccttcattt ttgatgcgaa gcataatacc tgcaaagtta aataaccaac
     1141 agtcctgcac caggacgtat cccttaattg tgcatagtcg caacagcatg cacaattaag
     1201 ggatagcctt attgaacacc cggagtggtt gcgggtgagg aggaacaatg aaaattaccg
     1261 tattgggatg cggtgcctta gggcaattat ggcttacagc actttgtaaa cagggtcatg
     1321 aagttcaggg ctggctgcgt gtaccgcaac cttattgtag tgtgaatctg gttgagacag
     1381 atggttcgat atttaacgaa tcgctgaccg ccaacgatcc cgattttctc gccaccagcg
     1441 atctgctcct ggtgacgctg aaagcatggc aggtttccga tgccgtcaaa agcctcgcat
     1501 ccacactgcc tgtaactacg ccaatactgt taattcacaa cggcatgggc accatcgaag
     1561 aattgcaaaa cattcagcag ccattactga tgggcaccac cacccatgcc gcccgccgcg
     1621 acggcaatgt cattattcat gtggcaaacg gaatcacgca tatcggtccg gcacggcaac
     1681 aggatggcga ttacagttat ctggcggata ttttgcaaac cgtgttgcct gacgttgcct
     1741 ggcataacaa tattcgcgcc gagctgtggc gcaagctggc cgtcaactgc gtgattaatc
     1801 cactgactgc catctggaat tgcccgaacg gtgaattacg tcatcatccg caagaaatta
     1861 tgcaaatatg cgaagaagtc gcggcagtga tcgaacgcga agggcatcat acttcagcgg
     1921 aagatttgcg tgattacgtg atgcaggtga ttgatgccac agcagaaaat atctcgtcga
     1981 tgttgcagga tatccgcgcg ctgcgccaca ccgaaatcga ctatatcaat ggttttctct
     2041 tacgccgcgc ccgcgcgcat gggattgccg taccggaaaa cacccgcctg tttgaaatgg
     2101 taaaaagaaa ggagagtgaa tatgagcgca tcggcactgg tttgcctcgc ccctggtagt
     2161 gaagagactg aagccgtcac cacgatcaac ctcttggttc gcggcggtat caaagtcacc
     2221 actgccagcg tcgacagcga tggcaacctg gcaattacct gctcgcgcgg cgtgaagctg
     2281 ctggcggatg cgccgctggt cgaagtggct gatggcgaat atgacgtgat cgtgctgcct
     2341 ggtggcatta aaggcacgga gtgttttcgc gatagcactc tgctggttga aaccgttaaa
     2401 cagttccacc gttccgggcg tatcgtcgcg gctatttgcg ccgcgccagc caccgtgctg
     2461 gtgccgcacg atatctttcc gattggcaat atgaccggct tcccgacgct gaaagacaaa
     2521 attcccgctg aacaatggca ggacaagcgc gtcgtctggg atgcacgggt aaaattgctg
     2581 accagccagg ggccgggtac agctatcgac tttggtctga aaattatcga cctgctggtt
     2641 gggcgcgaga aagcccatga agtggcatca caactggtga tggcggcagg gatttataat
     2701 tattacgagt agtgtcggat gcggcaaacg tcgcatctga ccagatgcga cgtaaaaact
     2761 acaaattacg ggcgatatac cttcacattg ttaaagcctt gctcgcgcag atagagcgcc
     2821 tgcagacggc tcatcacccc gcgctcacac cacagcagcc aggttttgtt ctggtcgaga
     2881 tcgccaaatt tggtgctcag tttatagaac ggcagagaaa ccacatcaat cccttcgact
     2941 ttcagtggct tatcttcctg ttcatcgaca gaacggatat cgagaatcac gtcgttcggg
     3001 ccgaagccgt tgacggtttc cacttccacc acttcctgct cggtctgctg ggcgatttcg
     3061 cggatatcaa cgttattcgc ttcctcaacc actttatcga gaatactgaa gtcgaacttc
     3121 tcttcttccg cttcaatctt cgatttaacc gctttcaccg tcgggctttt ggagatcacg
     3181 ccacaatatt ccggcatcgt gcgagcaaag tcttcggtgc caatctggcg agccaggttg
     3241 atgatgtgct ctttgtcgta agaaatcagc ggacgcagga tcagcgtgtc agagacgtta
     3301 tcaatcaggc gcaggttggt cagcgtctga ctggacacct ggccgagcgc ttcgccggtg
     3361 accagcgcct gtacgccgta acgttcagcc actttagatg cggcacgcac catcatacgt
     3421 ttgaggataa cgcccatctg accgtcgtcg attttctcga gaatttcccc gacaaccggt
     3481 tcgaaattaa tggcgacaaa acgcacgcgg tgggagctgc caaagcggtt ccacagataa
     3541 tgcgccacct gacgcacgcc aatttcatgc gctgcgccgc cgaggttaaa gaagcagtaa
     3601 tgcacgcggc agccgcgacg catcaacata taactggaaa caccggagtc gaaaccaccg
     3661 gaaatgagcg acagcacatc ttcctgggtg ccaatcggga aaccaccaat accttcgtag
     3721 cgacctttaa tcagcaggag gcgatcgtct tccacttcca gatgaacagt tacatccggg
     3781 ttagtcagct tcacgcgcgc ggattcaata tgctgattta aaccgccgcc gacgtaacgt
     3841 tccacatcaa tcgagctaaa atcatgtttg ccacggcgct tcacgcgtac gcagaaggtt
     3901 ttgccttcca gctgatcgcg atactgaacc aacgctttct caaaaatatc gtgcatgtcg
     3961 gtaaacggca cgtcttcgac ttcgagaata tggtggatac ccggaatacg ggtcagggcg
     4021 tcgcgaatag tcagacgctg gttttcatct ttcgcgcgaa cttcgatgtt atcccagtgg
     4081 cggacgacag cgagcgtctc atcatagtgc tttaaaacgt tacgaatgtt cccggtaagg
     4141 atttttataa agcgcaagcg cacagattgg cttttgatgg tgatttccgg gaacaattta
     4201 atgataaact tcatggcggc aatggttcgt tggcaagcct taagcgactt gtatagggaa
     4261 aaatacagca gcccacacct gcggctgcat ccaggcgcgg aagtatacca ctaacatcgc
     4321 tttgctgcgc acatcacctt accattgcgc gttatttgct atttgccctg agtccgttac
     4381 catgacgggg cgaaaaatat tgagagtcag acattcatta tgccgaagaa aaatgaggcg
     4441 cccgccagct ttgaaaaggc gctgagcgag ctggaacaga ttgtaacccg tctggaaagt
     4501 ggcgacctgc cgctggaaga ggcgctgaac gagttcgaac gcggcgtgca gctggcacgt
     4561 caggggcagg ccaaattaca acaagccgaa cagcgcgtac aaattctgct gtctgacaat
     4621 gaagacgcct ctctaacccc ttttacaccg gacaatgagt aatggacttt ccgcagcaac
     4681 tcgaagcctg cgttaagcag gccaaccagg cgctgagccg ttttatcgcc ccactgccct
     4741 ttcagaacac tcccgtggtc gaaaccatgc agtatggcgc attattaggt ggtaagcgcc
     4801 tgcgaccttt cctggtttat gccaccggtc atatgtttgg cgttagcaca aacacgctgg
     4861 acgcacccgc tgctgccgta gagtgtatcc acgcttactc attaattcat gatgatttac
     4921 cggcgatgga tgatgacgat ctgcgtcgcg gtttgccgac ctgccatgtg aagtttggcg
     4981 aagcaaacgc gattctcgct ggcgacgctt tacaaacgct ggcgttctcg attctaagcg
     5041 atgccaatat gccggaagtg tcggatcgcg acagaatttc gatgatttct gaactggcga
     5101 gcgccagcgg tattgccgga atgtgcggtg gtcaggcatt agatttagac gcggaaggca
     5161 aacacgtacc tctggacgcg cttgagcgta ttcatcgtca taaaaccggc gcattgattc
     5221 gcgccgccgt tcgccttggt gcattaagcg ccggagataa agggcgtcgt gctctgccag
     5281 tactcgacaa gtacgcagag agcatcggcc ttgccttcca ggttcaggat gacatcctgg
     5341 atgtggtagg agatactgca acgttgggaa aacgccaggg tgccgaccag caacttggta
     5401 aaagtaccta ccctgcactt ctgggtcttg agcaagcccg gaagaaagcc cgggatctga
     5461 tcgacgatgc ccgtcaggcg ctgaaacaac tggctgaaca gtcactcgat acctcggcac
     5521 tggaagcgct agcggactac atcatccagc gtaataaata aacaataagt attaataggc
     5581 ccctgatgag ttttgatatt gccaaatacc cgaccctggc actggtcgac tccacccagg
     5641 agttacgact gttgccgaaa gagagtttac cgaaactctg cgacgaactg cgccgctatt
     5701 tactcgacag cgtgagccgt tccagcgggc acttcgcctc cgggctgggc acggtcgaac
     5761 tgaccgtggc gctgcactat gtctacaaca ccccatttga ccaattgatt tgggatgtgg
     5821 gacatcaggc ttatccgcat aaaattttga ccggacgccg cgacaaaatc ggcaccatcc
     5881 gtcagaaagg cggtctgcac ccgttcccgt ggcgcggcga aagcgaatat gacgtattaa
     5941 gcgtcgggca ttcatcaacc tccatcagtg ccggaattgg tattgcggtt gctgccgaga
     6001 aagaaggcaa aaatcgccgc accgtctgtg tgattggcga cggcgcgatt accgctggca
     6061 tggcgtttga agcgatgaat cacgcgggcg atattcgtcc tgatatgctg gtggtcctca
     6121 acgacaatga aatgtcgatt tccgaaaatg ttggcgcgct taacaaccat ctggctcagc
     6181 tgctttccgg taagctttac tcttcactgc gcgaaggcgg gaaaaaagtt ttctctggcg
     6241 tgccgcccat taaagagctg ctcaaacgta ccgaagaaca tattaaaggc atggtggtgc
     6301 ctggcacgtt gtttgaagag ctgggattta actacatcgg cccggtggac ggtcacgatg
     6361 tgctggggct tatcaccacg ctaaagaaca tgcgcgacct gaaaggcccg cagttcctgc
     6421 atatcatgac caaaaaaggt cgtggttatg aaccggcaga aaaagacccg atcactttcc
     6481 acgccgtgcc taaatttgat ccctccagcg gttgtttgcc gaaaagtagc ggcggtttgc
     6541 cgagctattc aaaaatcttt ggcgactggt tgtgcgaaac ggcagcgaaa gacaacaagc
     6601 tgatggcgat tactccggcg atgcgtgaag gttccggcat ggtcgagttt tcacgtaaat
     6661 tcccggatcg ttacttcgac gtggctattg ccgagcaaca cgcagtgacc tttgccgccg
     6721 gtctggcgat tggtggatac aaacccattg tcgcgatcta ctccaccttc ctgcaacgcg
     6781 cctatgatca ggtgctgcat gacgtggcaa ttcaaaagct accggtcctg ttcgccattg
     6841 accgcgcggg cattgttggt gctgacggtc aaactcacca gggcgctttt gatctctctt
     6901 acctgcgctg cataccagaa atggtcatta tgaccccgag cgatgaaaac gaatgtcgcc
     6961 agatgctcta taccggctat cactataacg atggcccgtc cgcggtgcgc tacccgcgcg
     7021 gcaacgcggt tggcgtggaa ctgacgccgc tggaaaaact accaattggc aaaggcattg
     7081 tgaagcgtcg tggcgagaaa ctggtgatcc ttaactttgg tacgctgatg ccagaaacgg
     7141 ccaaagtcgc cgaatcgctg aacgctacgc tggtcgatat gcgttttgtg aaaccacttg
     7201 atgaagcgtt aattctggaa atggccgcca gccatgaagc gctggtcacc gtagaagaaa
     7261 acgccattat gggcggcgca ggtagcggag tgaacgaagt gctgatggcc catcgtaaac
     7321 cagtacccgt gctgaacatt ggcctgccgg acttctttat tccgcaagga actcaggaag
     7381 aaatgcgcgc cgaactcggc ctcgatgccg ccggtatgga agccaaaatc aaggcctggt
     7441 tggcataatc cctactccac tcctgctatg cttaagaaat tattcatggc aggagtggca
     7501 gcatgcaata caacccctta ggaaaaaccg accttcgcgt ttcccgactt tgcctcggct
     7561 gtatgacctt tggcgagcca gatcgcggta atcacgcatg gacactgccg gaagagagca
     7621 gccgtcccat aattaaacgc gcgctggaag gtggcataaa tttctttgat accgccaata
     7681 gctattccga tggtagcagc gaagagatcg ttggtcgcgc actgcgggat ttcgcccgtc
     7741 gtgaagacgt agtcgttgcc accaaagtgt tccatcgcgt tggtgattta ccggaaggat
     7801 tatcccgtgc acaaattttg cgctctatca acgacagcct gcgacgtctc ggcatggatt
     7861 atgtcgatat cctgcaaatt catcgctggg attacaacac gccgatcgaa gagacgctgg
     7921 aagccctgaa cgacgtggta aaagccggga aagcgcgtta tatcggcgcg tcatccatgc
     7981 acgcttcgca gtttgctcag gcgctggaac tacaaaaaca gcactgctgg gcgcagtttg
     8041 tcagtatgca ggatcactat aatctgattt atcgcgaaga agagcgcgag atgctgcctc
     8101 tgtgttatca ggagggcgtg gcggtgattc cgtggagccc gctggcgcgg gggcgactga
     8161 cgcgtccatg gggagaaact accgcacgac tggtgtctga tgaggtgggg aaaaatctct
     8221 ataaagaaag cgatgaaaat gacgcgcaga tcgcagagcg gttaacgggc gtcagtgaag
     8281 aactcggtgc aacacgagca caagttgcgc tggcctggtt gttaagtaaa ccgggcattg
     8341 ccgcaccgat tatcggaact tcgcgggaag aacagcttga tgagctgctg aacgcggtgg
     8401 atatcacttt gaagccataa cagattgccg agctggaaac gccgtataaa ccgcatgcgg
     8461 tcgtaggatt taaataagag tccaggcctg atgagacgtg acaagcgtca catcaggcat
     8521 cggtgcacaa ctacgacaga atacccagcg gccagtgatg accgataaag tacaggatgc
     8581 ctgcggaaat caccccggcg acaatatcgt cgatcatgat ccccatgccg ccatgtacat
     8641 tgcgatcaaa ccagcggatc ggccacggct tccacatatc cagaatacgg aaaatcacaa
     8701 acccggcggc aacccactgc cagtcattgg tcggcagcgc catgagcgtg atccacatac
     8761 caataaattc gtcccagaca atgctgccgt gatcgtgcac gcccatatct ttcgccgttt
     8821 gatgacaaag atagacgccg atacagatcc ccaacatcac caccagcgag tagagctgcc
     8881 agggtaaaaa ggtcatcaga taccagaaag ggatcgccgc cagcgatccc atcgagccag
     8941 gaacgattgg gcttaatcca cttccgaatc cgacagcaag cagatgccac ggattactca
     9001 tcttcaggcg acttttcgcg acatctttat ggcgtgacaa aatggtcata tcctttccag
     9061 tcaaacgtga caggttcgcc gtcacgaata aaacaaagcc cttcgatatc ggcggtcatt
     9121 tgcccgatac aggtaaacgg tacgccaagg tgtccgagag caacatccag cgcgccacgg
     9181 ttcagttccg gcaccgtgaa acacaactcg taatcttcac cgccagagag cgcccagcgc
     9241 agcgcctgtt ccggttcaac atggcgagaa agcgcatcag aaaacggcag caatgccagg
     9301 tcaatacgtg cgccgcagtc gctggctttc acgatatgcc cgagatcgga aatcagaccg
     9361 tcagagagat cgatagctga atttgccaga tcgcgcagtg cctgcccctg taaaatacgc
     9421 ggcgatggac ggagatgacg tttgatcaag tagtcagcat ctttagcatc ggcaacctgc
     9481 aaacggtttt gcaaaatcgc cagcccggcg gcgctatcgc ccggtgtacc ggtcacatag
     9541 atccagtcac ccggtttcgc cccagagcgc gttaaggctc gtcccatcgg aacaaagccg
     9601 tggataccca acgtcattga taatggccca cgcgtggtat cgccgccaat gagttgcata
     9661 tcgtaataat tgagaagatc aaacaaactg tcgctgaagg actcaagcca cgcttcgtct
     9721 acgtccggta aggttaatgc cagcgtcagc caggccggat cggcccccat cgctgccaga
     9781 tcgcttaggt tcaccgccag tgctttataa gccagatcag caggatcgat atcagggagg
     9841 aaatggttgc ccgccaccag cgtatcagtg ctgatcgcca gggtctgttt ctcggggata
     9901 ttgagaagag cgcaatcgtc gccgatgccc agttcgacat caagacgaga acttcttaca
     9961 cggtcaaaat aacgggcaat cagggagaac tcgccacatg ccatacgtta tgcctcagca
    10021 ggtaaaaaag aaaaggccgg cgatcatgga acagtcttcc gtgaatctac cggcctggat
    10081 attacttttt gttagggcga atcacaggtg ctgctttatc gagtacgccg ttgacgaact
    10141 tatggctgtc ttctgcgccg aacgatttcg ccagttcgat cgcttcgtta atggccactt
    10201 tgtatggcac atcactacgt ttagacagtt cgtacagcgc aatgcgcagt actgcttttt
    10261 ctacctggcc cagttcttcc agcaggcggg acaggtaggg cttcatcagt ccgtcgaggt
    10321 atgcggtgtt agtcgccacc ccggccagca gctcacggaa gtacaggacg tcaacgtctt
    10381 ttacatcctg ttcagccagg aactggtatt caacatcagc gatgtcgttc tgggacaact
    10441 gccaggagta gagcgcctgg acggcacact cacgagcgcg gcgacgagca gcaggtttca
    10501 cggatttccc cttactaatt tcaggccttg atggctttca atacattaat catttcaagc
    10561 gcggtcagtg cagcttctgc acctttgttg ccagctttgg tgccagcacg ttcgatcgct
    10621 tgttcaatgc tttcagtggt cagaacccca aaagcaaccg gaatttcgct gtcctgggca
    10681 acatgcgcca ggccgttgct tgcaccacca gcgacatatt caaagtgggc agtgccacca
    10741 cgaataaccg tacccagcgc aatcaccgcg tcgtatttac cggttttagc cagtgcaccc
    10801 gccgccagcg gcagctcata ggcaccaggc acccaaacaa cggtaatgtt ttcatctttt
    10861 acctgaccga tacgtttcag tgcgtcaatt gcaccttcca gcaggctgtc attgataaag
    10921 ttgttgaaac gcgcgatggt gatggcgacg cgagcgtccg gggtagcaac gttagcttca
    10981 ataatgttca ttttcttcct tcgggttcga gtatggcccc gcaggggggc ggattttagc
    11041 ataatatttc gtgcgctgct tccctttcga gccgggagat catgcaccca ctaaatgcag
    11101 gcaaacatcc gggcctacat gacgtatctc tttgaattta aattgggggg cgtcggctaa
    11161 tttctcaagc cctggcagcg agcataatcc acgggcgtcg ctgcctaata gtttaggtgc
    11221 gatatagaca atcagctcat cgactaaacc cgcctgtagc aatacgccag cgagcgttgg
    11281 ccccgcttcc acccagatgc tgttaatctg ctgtttaccc agttgcatca tcagtacaac
    11341 cagatccaga tgacctttat gctctggaat cagcaaggta cgcaccgttt ccggccactc
    11401 acgagaatct tcctgcgtac gcgcgaacca ggtttcgccg ggctgctgca caatgcgatg
    11461 ttccggcgtc acgcgatttt ggctatcaat cacaatacgt actggctggc ggagattttg
    11521 ttgcgggtag agcgcctgag tttgttcatc cagttcagac caacgcactg ttaaggctgg
    11581 atcatccgcc agcaccgtgg cgctgctggt taaaatggca tgactttgtg cgcgctgtcg
    11641 ttgtacgtcg cgccgcgcct gaggcgaagt gatccactgg ctttcgccgc tcgccatcgc
    11701 cgtgcggcca tcaagcgatg cgccaagttt taactgaata taaggaaagc cggtgcgcat
    11761 ccgcttgaga aagcctttat tcaattgctc ggcttcactc atcatcagcc cgtggctgac
    11821 gtcaatgcca gcctgttgca gacggtaaag tccacgccca gcgacctgcg ggttaggatc
    11881 ttgcatcgct gcgaccacgc gcgctacgcc agcggcgatg agtgcgtcac agcacggtgg
    11941 cgtacggcca tgatggctac agggttcgag tgtcacatac gccgttgcgc ctttggcttt
    12001 ttcacccgcc atacgcaacg cgtgtacttc ggcatgtggt tcacccgctc gttggtggta
    12061 accttcaccg acaatttcgc catctttgac aatgacgcac ccgacattcg ggttggggtg
    12121 ggtggtaaaa cgtcctcgtt gcgccagctt tagcgcccgc gccatgtaat actcgtcctg
    12181 cacggcttag tcctccaggc gcgcgatctc ttcgccaaat tctttgatat cttcgaaact
    12241 gcggtagaca gaggcaaaac ggatataggc gactttatcg agctttttca attgctccat
    12301 caccagattg ccaatcatct tgctcggtac ttcgcgctca ccggtggcgc gcagctgcga
    12361 tttaatatga ttgattgcca tttcaacgta atcggaactc accggacgtt tttccagcgc
    12421 ccgcagcatt ccgctacgca atttctcttc attaaacggt tcacgcacgt cgttgctttt
    12481 tacaacacgc ggcataacca gctccgccac ttcaaaggtg gtgaaacgtt cattacacac
    12541 cagacactgc cgacggcggc gtacggatga accctcgccc acgagacgag agtcaattac
    12601 cttagtgtcc acggcgaaac agaatgggca atgcatacgg cgtcctgacc aggtggttaa
    12661 cggaaatcta ttttaccctg aactgacgat acaacaaagg cgcaacgctt ttgagataaa
    12721 ggatcgatgc tttcgctgct tttaccctct accattaggc taattgcgac gagaaaggga
    12781 cgacctatga atactaacgt ttttcgactg ctcctgctgg gaagcctgtt cagccttagc
    12841 gcctgtgtgc agcaaagtga agtgcgacag atgaaacaca gcgtcagcac gctgaaccag
    12901 gagatgacgc aactcaatca agaaacagtc aaaatcactc agcaaaacag gctgaatgca
    12961 aaatccagca gtggggttta ccttctgcct ggagcgaaaa caccggcaag actggaaagc
    13021 cagatcggta ctttacgtat gtcgctggtg aatattacgc ctgatacaga tggcaccacc
    13081 cttacactac gtattcaggg tgagtcgaat gatcccttgc ccgcattcag cggaactgtt
    13141 gaatatgggc aaattcaggg aacaatagac aactttcagg aaatcaatgt gcagaatcaa
    13201 ttgattaatg cccctgccag cgtcctcgcc cccagcgatg ttgatattcc gttacaatta
    13261 aaggggattt ctgtggatca gttaggcttt gtacgtatcc acgacattca acctgttatg
    13321 cagtaaacgt atttcgggac gattttgtgc gtcccgcaac atcttccccg tcattttgtt
    13381 actctgctta catcacctgg attgatagta aaagtttgca acaagggcga aagtcagtac
    13441 aatccccgcc cgaatgtgtg taaacgtgaa cgcaatcgat tacgtaaatg atagaactgt
    13501 gaaacgaaac atatttttgt gagcaatgat ttttataata ggctcctctg tatacgaaat
    13561 atttagaaac gcaatttgcg cctttttcac tcccgcaagg gattttcaaa cagtggcata
    13621 catatgaaaa aaacattact ggcagccggt gcggtactgg cgctctcttc gtcttttact
    13681 gtcaacgcag ctgaaaacga caaaccgcag tatctttccg actggtggca ccagagcgtt
    13741 aacgttgtcg gaagctatca cacccgtttc ggaccgcaga tccgcaacga tacctacctt
    13801 gagtacgaag cattcgctaa aaaagactgg ttcgacttct atggttatgc ggatgcgccg
    13861 gtattcttcg gcggtaactc cgatgctaaa ggtatctgga accacggttc tccgctgttt
    13921 atggaaatcg aaccacgttt ctccatcgac aagctgacca atactgacct tagcttcggt
    13981 ccgttcaaag agtggtactt cgcgaacaac tacatttacg acatgggtcg taataaagat
    14041 ggtcgccaga gcacctggta catgggtctg ggtaccgata tcgacactgg cctgccgatg
    14101 agcctgtcca tgaacgtcta tgcgaaatac cagtggcaga actatggcgc agcgaacgaa
    14161 aacgagtggg acggttaccg tttcaaaatt aaatactttg tgccgattac cgatctgtgg
    14221 ggcggtcagc tgagctacat cggcttcacc aacttcgact ggggttccga tttaggggat
    14281 gacagcggta acgcaatcaa cggtattaag acccgtacta ataactctat cgcttccagc
    14341 catattctgg ctctgaacta cgatcactgg cactactctg tcgtagctcg ttactggcac
    14401 gacggtggtc agtggaacga cgatgcagaa ctgaacttcg gcaacggcaa cttcaacgtt
    14461 cgctctaccg gctggggtgg ttacctggta gtaggttaca acttctgatt atgaaaatgc
    14521 cgggatttat tcccggcatt tctgattgtt aacaacgtgt gctttgttca tgccggatgc
    14581 ggcgtaaacg ctttgtccgg cccacaaaag cacgtaaatt caatatattg ctgaaataac
    14641 gtaggcctga taagcgtagc gcatcaggcc attttacgtt ttaatcactt cttcttgttc
    14701 atcatcgctt tcaggtcagc gaatgggttg tacttcgctt caccgacatc tttctgcgca
    14761 tcttcccctg cgataacggt cgtaccatac tgatccgctt cggtatattt cgaatgctca
    14821 tgatcgtggc aatagagaca caacaattcc cagttactgc catcttccgg gttattggta
    14881 tggtcgtgat caatgtggtg aacggtaagt tcacgcaggt tagaataaac aaactcgcgg
    14941 gaacagcgac cgcagaccca cggatagatt tttaatgctt tttcgcgata gccgctttct
    15001 aaccgtgcat agttttttgg gatgatagcc atgaaaagtt acctggatga aatgtagggt
    15061 tacgcgcagt ttcccaagat gcggggattt tcgcaatctc catacatcaa aaaaaatccc
    15121 gatcttctga ccgggatttt caacatcaac gggaacttga ttaaggcaga attgacggct
    15181 gatccgcccc ttctttttcc actttctgct gcaacatgtg ttcgcgcttc atacccagtt
    15241 tcagagccaa cgcagatgct acgtagatgg aagatgcagt gccgatggaa acaccgataa
    15301 gcatggtcag cgagaagcct tccagtaccg gaccaccgaa gagatacagc atcaggataa
    15361 ccatcaaggt agtaccggat gtgatcaagg tacggtgcag cgtctgggtc aaggacacgt
    15421 taaagatttc gtaaggcgta ccgcgacgga tcttgcggaa gttttcacga attcggtccg
    15481 ataccacgat actgtcgtta agcgagtaac cgataaccga catcaacgat gccacaatgg
    15541 tcaggtcaat ctcgatatgg aataacgaca aaatacccag cgtaatgata acgtcgtgcg
    15601 tcagcgcaat aaccacccct gccgccagtc gccactcaaa gcggaaacct acgtacacga
    15661 ggatagacag cagcgctgcc atcaacgcca tcgcaccggt ttgcgcaagg tcagccccca
    15721 cgctcggacc gacgaactca atacgcttca ctgctgcatt ctgattggtg gattcgttaa
    15781 tcaccttcag aacttggctg cccagcacct gaccgccggt ttcgccttca gcaggcggca
    15841 tacggaccat gatgtcgtgg ctgctaccaa agttttgcag cattggctct tcaaaaccgg
    15901 ctttttgcaa tgcatcacgc attacgtcaa tttcagccgg tttttcgagc gtaatttcga
    15961 taaccgtacc accggtgaaa tccagccccc agttaaagcc gcgcacgccc ataataacga
    16021 tagcagcgat taacagcaga ccagagatgc cgaaagccca gtagtcccag cgcataaagt
    16081 catagacttt acggccgtgg tttagttgtt caacagtata ttcctgtgcc acatcgcact
    16141 cctcagattg acagcttctt gacgcgcttg ccgccatata gcaggtttac gatggcacgc
    16201 gtaccgacaa tcgcggtaaa catcgacgtc gccacaccga taccggtagt aatcgcgaac
    16261 cctttaattg ccccggtacc cactgcgtac aggatgatga ctttaatcag cgtggtgatg
    16321 ttcgcatcga agatagaact gaatgcgcca cgataacctt catcaattgc ctgttgaaca
    16381 gtacgtccgt tgctcaactc ttctttaata cgttcgttga tcagtacgtt cgcatcgacc
    16441 gctaccgcaa gggttaagac gatacccgcg atacctggca tactcagtgt tgcgcctggc
    16501 aacagcgaca taataccgac gatcaagatc aagttggcaa tcagagcact ggtcgcaatc
    16561 agaccaaact tcttatagaa gatgatcatg aacagaatag aaaccagcag accggcaagg
    16621 caagcctcca gcccctgctc aatgttctgc atacccaggg tcgggccaat tgtacgttct
    16681 tcaacaatct gaatcggcgc gatcaacgca ccggcacgca gcagcagtga cagctgacgg
    16741 gcttcgttcg ggttgttgat gccggtgata cggaagctgt tacccagacg agactggatg
    16801 ttggcgatgt taatcacctc ttcctgtttc accagaaccg cacgaccatt tgcatctttc
    16861 ttaccgctgt ctttgtactc cacaaacagg gttgccatcg gtttgccgat gttgtccttg
    16921 gtgaagttag acataatgtt gccaccagcg ctatcgagcg agatgttaac ctgcggctgg
    16981 ttgtattcgt cctggctgga agtggagtcg gtgatatggt caccagtcag aattacgcgt
    17041 ttgtacagca caaccggctg accttcgcgg gtctgtttca cttcagagtc gcccggtacg
    17101 cgaccggatg ccgcagcggc ctgatcaaca ttggtgttta ccagacggaa ttccagcgtt
    17161 gccgtcgcgc ccagaatctc tttcgcacgc gcagtgtcct gaataccggg cagttcaaca
    17221 acgatacggt cagcaccctg acgctgaacc accggctccg ccacgccaag ttggtttaca
    17281 cggttacgca ggatattaat gttctgctgc accgcatatt cacgcgcttc actcagacga
    17341 gcatcgctca ttaccgcacg cagctggttg ctgccctggc tgctaatcac caggtccgga
    17401 tggcgcttgc tcagatacgc aatggcttca tcacgagctt tagcatcgcg gaaagtgatg
    17461 ctcaggccgt agttgttttc tttacgaaca gtggtatacg ggatgccctt ttcgcgcagg
    17521 tcactgcgta ggctatcgat attttgttcc tggagtttgc caagcgcggt gtccatatcc
    17581 acttccatca ggaagtgaac gccgccacgc aggtcaaggc cgagcttcat cggctcagca
    17641 tgaatagctg ccagccagcg cggcgttgcc ggggcaaggt taagcgccac gacgtatttg
    17701 tcacccataa cgcccattaa tgcttcacga gcgcgcaact gggtgtcagt ggagtcgaag
    17761 cgcgcaagaa tagcgccctc ttccagtgcc acagacttag cagttatttt ttcttcttgt
    17821 aacgttttct ggacctggat cagcgtttgc tcactggcgg cgactccgcg cgcaccagtg
    17881 atctgaacag ccggatcctc accaaacagg ttgggaagcg catacagcag accgatgaca
    17941 atcaccacga tcagcatgac gtacttccac aaaggataac ggtttaacac ggcaattccc
    18001 ttagggaaaa attttaatta cagcgccttc atggtgcctt tcggcaggac ggcagctacg
    18061 aagtcacgtt taataactac ttcagtggtg tcattcagcg cgatagcaat gtagccgttt
    18121 tccgctactt tggttacgcg accaaccagg ccaccgttcg tcagaacttc atcacctttg
    18181 gcaatggagt ccatcagctt tttgtgttct ttggtgcgct tctgctgtgg acgcaggatc
    18241 atgaaataga aaatcagacc gaataccacc agcatcaaaa tcaaagacat cgggctacct
    18301 tgcgccggtg cacccgttgc cgctaccgca tcagaaataa aaaagctcat taaatttccc
    18361 tcattattaa tattaatcaa cgttcaaagg tggtacttct cgcccctgac gctggtaaaa
    18421 atcagttacg aagctctcta atttaccctc ttcaatagcc ttgcgtaaac ccgccatcaa
    18481 acgctggtag taacgaaggt tatgaatggt gttgagtcgc gcgcctaata tttcgttgca
    18541 acggtcgaga tgatgcaagt aagcgcgtga ataattgcga caggtgtagc aatcacactc
    18601 aggatcgagt gggccagtat cgctcttata tttcgcattg cggattttca ccacgccatc
    18661 ggtcacgaac aaatgaccat ttcgggcgtt gcgggttggc attacgcagt caaacatatc
    18721 gataccgcga cgtacgcctt caagcaggtc ttccggttta ccaacgccca tcaggtaacg
    18781 cggtttgtct gccggaattt gcggacacac atgctccaga atacggtgca tatctgcttt
    18841 cggctcaccc acagccagac cgccgacagc gtagccatca aagccgatat ctaccagacc
    18901 tttaacagat atatcacgta aatcttcgta aatactgccc tgaattatac caaataacgc
    18961 atttttgtta ccaagactgt caaaacgctc acggctacgc ttcgcccaac gcagagacat
    19021 ctccatagag cgttttgcgt aatcccagtc agcaggatac ggcgtacact catcaaagat
    19081 catgacgata tccgaaccaa gatcgtactg aatctccatc gacttttccg ggtcgaggaa
    19141 aatcgggtcg ccgttgatcg ggttacggaa gtgaacgccc tgttcggtga ttttacgaat
    19201 atcaccaagg ctgaagacct ggaagccgcc ggagtcggta agaatcggtc ctttccactg
    19261 cataaaatcg tgcagatcgc cgtgcagttt catgatttcc tggcccgggc gcagccacag
    19321 gtggaaggtg ttgccgagga taatttgcgc gccagtggct tcaacttctt ccggcgtcat
    19381 cccttttacg gtgccgtagg tgccaacagg cataaaacaa ggcgtttcca ctacgccacg
    19441 atcaaagacc aggcggccac ggcgtgcgcg accgtcggtg gtgtccagtt caaatttcat
    19501 tttttctcca ctacgtcaga aaaacagtcc aacgtttaaa ccagcgccgc ggaattactc
    19561 cccgacgcgc tcattaattg cctgcggatt gtacgtgata aacatcgcat caccgtaact
    19621 aaaaaagcga tatttctctt ctaccgccgc tttataggca ttcatggtgt gttgataacc
    19681 ggcaaaggcc gaaaccagca taatcagcgt cgactctggc aagtggaagt tcgtcaccag
    19741 cgcatcgacc actttgtact ggaagcccgg atagataaag atttgggtat cgtcaaagaa
    19801 cggttcgata agatcgtttt ttgccgcctg tgccgcgctt tccagcgaac gtactgaagt
    19861 ggtgccaacc gcaatcaccc ggttaccgcg cgctttcgcc gccagtaccg cgtctaccac
    19921 atcctgcggt acttcagcgt attccgagtg catgatgtga tcttcaatgg tgtcgacgcg
    19981 caccggctgg aaggtgcccg caccaacgtg caacgtcaca aacgccatct ccacgccttt
    20041 ggcgcgtaat ttttccagca aaggctcgtc aaaatgcaga ccagcggtcg gggctgcaac
    20101 cgcgcccggt ttttcgctat aaacggtttg ataaagttcg cggtcagcgt cttcgtccgg
    20161 acgatcgata tacggcggca gcggcatatg gccgatgctg ttgaggatat ccagtaccga
    20221 gcgttcatca ttaaattcga cttcaaacag tgcgccgtgg cgcgcggtca ttgttgcgtt
    20281 gatactttcg tcatcgccca gcaacagttc tgcgccaggt tttggcgctt tcgaggcgcg
    20341 aatatgcgca agaatgcgtt tgtcgtcgag catccgctca accagcactt caatcttgcc
    20401 gccgctggct ttacgcccaa acagacgcgc cgggatcacg cgggtattat taaaaaccag
    20461 aagatcgccg gggttgagct tatcgagtaa atcggtgaaa gtaccgtgcg tcagcgcgcc
    20521 cgtcggcccg tccagcgaca gtaaacgaca gctactgcgt tcaggcatgg gatagtgggc
    20581 aatcagggat tcgggcaatt caaaggagaa atcggtaacg cgcatgacac tgactcagac
    20641 taaaataaga gtcgggtagt ctagtgccgg ggcgctctcc ctgcaacctt tacccccctt
    20701 tggatagaat ataatcgatg aattttttag ctcacctgca tttagcccat ctcgcggaaa
    20761 gctcgctttc tggcaattta ctggctgatt tcgtacgcgg aaatcctgaa gaaagttttc
    20821 cgcccgacgt cgtggctggc attcatatgc atcgacgtat tgactgacaa tctgccggaa
    20881 gtccgcgaag cacaggagtg gtttcgtagt gaaacgcgcc gcgttgcgcc tattacgctg
    20941 gatgtcatgt gggatcactt tctttcccgc cactggtcgc agctgtcgcc ggactttccg
    21001 ctacaggaat ttgtctgtta tgcccgcgag caagtgatga cgattttgcc ggactcaccg
    21061 ccacgtttta tcaatctgaa caattacttg tggtcagagc agtggctggt gcgctatcgc
    21121 gatatggatt tcatccagaa cgtgttaaac ggcatggcaa gccgccgccc acgtctggat
    21181 gccctgcgtg actcctggta cgatttagac gctcattatg acgccctcga aacccgcttc
    21241 tggcagtttt atccgcggat gatggcgcag gcgtcacaca aggcgttata aaacgttagt
    21301 tcatccatac cgtagccgaa atggcaggca aagccagaat cccgtcagtc agttgcccat
    21361 gcccttcttt gcattgccat tgcacggcat tgagaaacgg tgacgcgggt agcaccactt
    21421 cactggcctc gccacggttg attgccacca gtacacgttg ctgattcagc acgcggacaa
    21481 ataccactac gttatcttcc gcatacagca cctgacagcc gccacgacgc agcgcctgac
    21541 ttttcttacg cagcacaatc attcgccggt acagcgcgaa taacgccgta tcctgctttt
    21601 ccacctgcca ggggaacggt ttacggcaaa acggatcgtt tttaccatcc agtcccactt
    21661 catcaccgta ataaatgcac ggtacaccag gccaggtgaa cagccagacc accgccagcg
    21721 gcaggcgcgc aatatcccga ccgagcagcg ttttaaatcg cgcagtatcg tggctatcga
    21781 gctgattaaa catacgtaat tgttgttgat gagaaagccc tgcgcggtaa ttatccatcc
    21841 aggccataca ggtttgggcg tcaatttgct gcggatcata agagatgtcg gtattggcaa
    21901 gaaatcccca caacgggaat gtgaagccac gatagttcat ggcagcatct tccacatcgg
    21961 cctgtaacca ttgccgtgca tcgccaaaat gttcgccgac aatatacgct tccggctggg
    22021 tttctttcgc cgcttcggtg atcccggcaa cgtgctgcat attattgcgc gccccacccg
    22081 cctcccccag catatgcacc acatccagcc gccagccgtc catattccac ggcgctttca
    22141 gccagtggcg gacaatactg tcttccccgc gataaatttc attcaccaga ctttccgact
    22201 gataatccag cttcgtcaag ctggcatagc caagccagtc gagcgccgtg ccatcatcac
    22261 taaacgagta ccagtcgcgc cagggcgatt cagagttgtg acaagctccc cccgtgccaa
    22321 gattgtgcct gtcaaaccag gcatgggaat cgccactgtg gttaaacacg ccgtccagca
    22381 ccagccgcat tcccagctgc tgcgtattgt gtcgcaaacg cagcaacgcc ccatcaccgc
    22441 caaactgcgg atcgacatgg cgataatcct cggtatcgta tttatgtacg ctgggagctt
    22501 taaacaccgg attgagatac agcgctgtca cgccaagctt tttcagatac ggcagttttt
    22561 cgcttatccc gtccagatcg ccgccataga acgttgatcc gcccgcctgc gccgtgaccg
    22621 gttcatccca gtcacgcaag atgatctctt gtccggctgc atgatggtaa tagacatgat
    22681 cctgttcagc ttcacgagga agactacgcg caaaacgatc agggaagatc tgataaaaaa
    22741 tctgatccgc agcccattgt gggccgatat caggtacatc gacggcaaac tgctccagtc
    22801 gtgccggcgg cattcggctg aagccctgcg gtgtaaacca acgctggcga tcgtgccaca
    22861 gcagtttgaa actgtaacgc cgccggggtt gtccgctgga gagatcaatc gccgcacgcc
    22921 atgcggtgac gcctggctgc ggctgactgc gctgcttatg cattggtact gacatttctt
    22981 cgttatcgtg ttctgtacgc agcataatgc gctgcggtgg gtcttcgccc gtcagccaca
    23041 gtgtaatgag cagttgatct ttgctttgtt taacaaatgg gggcaccggc aggtgccatg
    23101 catttaacat cataattccc ctgtgatgaa aatctgatca ccttgccata gcctaatgca
    23161 ggatcactca tcatctgaag gatatttggg ggaggaggac gggggcggat aactttcgga
    23221 atagccggat caggatttcc tcctgaaccg gcttacagaa cgcaagtatt attggttttc
    23281 agccagctga cgatcgtggc ggcgtttaaa catccagcca atcaacagca caacaatcca
    23341 cgcgaaaccg acatacagcg agatacgcgt atccgggtga taaccaatca acccgataat
    23401 aaagagcagg aaaatcagcc cgccgatggt cgttgctacc ccacccggca ctttaaattt
    23461 cagcgcctta acttcttctg gcggcaaacg gcgacggaag gcgatttgcg acagcaggat
    23521 cataatccac acccataccg tggcgaaggt tgccagcgaa gcgatcacca ggaagacgtt
    23581 ttccggcatg atgtagttca gatacaccgc aaacagcagc gcggtagtca tcaccagcac
    23641 cgtaacccac ggaataccgc gacgcgacgt tttgctgaaa attttcggtg cgctgccctg
    23701 ctctgccata ccgtggagca tacggcctac gccaaatacg tcactgttaa ttgccgacag
    23761 cgaagcggtc agcacaacaa agttaagaat gctggcggca aaggtaatgc ccatatgctg
    23821 gaacgtcagt acgaacgggc taccggcagt gccaacctga ttccacgggt agatagacat
    23881 aatgacgaac agcgtaccaa cgtagaacac cagaatacgc atcggcacgg agttaatcgc
    23941 acgcggaatc gatttctcag gatctttcgc ttcaccggcg gtaatcccga taatttcgat
    24001 cccaccgtaa gcaaacatca ccatttgcaa cgacattacc atgccaagcc agccgttact
    24061 gaagaagccg ccgttgctcc acaggttatg aataccggtc ggttgcccgc cgttgccaat
    24121 cccccagatg atgatgccga aaccggcgac aatcatgatg atgatggtgg cgactttaaa
    24181 gaacgagaac cagaattcca gctcaccgaa gaccttcacg ctcatcaggt ttacggcgca
    24241 aatgatcagc accacgctca gtacccaaat ccagtgcggc accgtcggga acaagacacc
    24301 catatagata ccaaaagcgg tcacatcggc gatggcgaca ataaggattt caaaacagta
    24361 ggtccatccg gtaatgtaac ctgccagcgg gccaaggttt tcctgcgcat aacgcgagaa
    24421 agagctggcg gccgggttat gtaccgacat ttcccccagc gcacgcataa tgatatacgc
    24481 cgcgatacca ccgataatat aggccaacaa cacgctcgga ccggccattt tgatggcgtc
    24541 tgccgaaccg taaaacagcc cggtgccaat tgctgaaccc agtgccataa agcgtatgtg
    24601 gcgggtactt agcccacgct ttagcttatt cttactttcc atcaattcct aacccatcaa
    24661 cacaataaaa aaccacgggg catcgcagcc ccgtggttaa aacaaatgtt cagtgattta
    24721 gtgagcgctg gaggtcacct gacgacctgc cgcgcgatcc cagataatgg ccagaaccac
    24781 catcaccact gttggcatta accacgccag accttgttcg gccagcggta aacgctgcgc
    24841 ccaggacggt aagatatcgc tgaatgcaga tgctttgatc ccgtcgagaa taccaaaaag
    24901 caggctgata aacatcggcg gagcaatcac gcgggacgaa ttatgccacc atgagcgtgt
    24961 aaaacttaat acaaccagtg cgatacacgg cggataaata gcggtcagca ccggtacgga
    25021 gatctggatc agctggctta agccgaggtt agacaccacc atcgagaagc cgccgaggat
    25081 aaacaccagc gtacgataag agagcggtac gtactgggca aagaattctg cacaagcaca
    25141 ggtcaggcca accgccgtga ccagacaggc gatgaagatt aacgccgcca gcaggaagct
    25201 accgccgccg ccaaaggtgt gctgaacgta agcatgcaga atagcagcgc cgtttgcaga
    25261 ctgatcgacc agcgacgcgc tgtctgaacc cagacagaac agcgccaggt acagcaaagt
    25321 cagaccaaca cccgccatca ggccagccca gacggtataa cgggtcagca gacgcgcttc
    25381 ggtaacgcca cgagaacgcg ccgcgttaac aataacgata ccaaacacca ttgcgcccag
    25441 cgtatccatg gtcagatagc cgttaacgaa gccgttagaa aatgcagcgt tttgataagc
    25501 ctcagtcgcc gtgctgatag aacccgccgg ccagacaatc gcgacaacag acaggatgac
    25561 cagcgcgata attttcagcg gcgcaaggaa gttgcccacg gtatccagca gcttgcccgg
    25621 atagagcgaa accagaataa cgatagcgaa atagaccagg ctgtagataa acagcggcag
    25681 cgcggaatca cccgtcagcg gcgcaatccc cacttcaaag gaaacggtag ctgtacgcgg
    25741 cgtagcgaaa agcggcccca ccgccaggta acaaaccgtt gccagcagta cgccagcgac
    25801 tttaccgatt ggcgtgctga ggctgtcaac accgccgcca acttttgcca gcgccactac
    25861 cgttaatacc ggcaggccaa cggcagtaat gaggaagccg aatgccgcag tccagacgtg
    25921 ttcgcctgcc tgtaagccga ccattggagg gaaaataatg ttacctgcgc cgacgaacaa
    25981 cgcaaatgtc ataaagccca gagcgatgat atcgcgcgat cttaattgat gggtcataaa
    26041 tcatactgcc tgtggatgtg gtgttgtaac gttaaatttt tgtgccaatc cctggggatg
    26101 atacagcggt atatttaaac aattttagcg ggaaatactc ccaaccaggc gcggcgaaga
    26161 atagtttttc gatttaccgc gttaatgcag tcagtatgac agcacctgaa gcgcaattta
    26221 aacgcttata acgttttaaa gcaaggaggg atctaaaaac cagaggtata tagcgataag
    26281 aaatcaccaa ccagtataaa acactatttc cgtgaaaaag acatggctaa tcatgcgaac
    26341 aaaaaatcac caaccgttgt aaaatctaac gcccgtattt tgactgcaaa gaaaataagc
    26401 cagcatcggc tggcttatgg caataaaaga tgacaaaggc ggattaatcg ctgtttttgg
    26461 caattaaacg ttccgggata acaaaactga aacgtgttcc ttttcctact gtactctcaa
    26521 tattcaggcg actttcgtga tgattcaccg catgtttcac gatcgctaac cctaatccgc
    26581 taccgccggt ttgccgggaa cgcgctttat caacgcgata aaaacgctcg gtcaggcgcg
    26641 gaatatgctc cggtgcaatg cccggtccgt tatcttcaac gctaaattcg gcaccgtgcg
    26701 gcactcgctg ccagcgtacg gtgatatgcg tgccttccgg cgtatgattc acggcgttat
    26761 agaccaggtt cgaaatcgca ctgcgtagct gatcttcgtt gccagacacc ttgaggccgt
    26821 tatctatctc aaaggtaaat gtctgttttt tctgactcag agtctgagcc tcgcgctcaa
    26881 caacgcgcag catcatcggc acatcaacct tttcattgag caattgcgtc ggcgcggctt
    26941 caattttcga cagcgttagc aactgcttca ccagcccttc catccgctgg gtctgctcgc
    27001 gcatggtgtg caacgctttt tcgcgtaccg cgccttccag cggctgctca tccatcatct
    27061 ccaggtaacc ctgtaacacg gtcaatggcg tacgtaactc atggctcacg ttggcaaaaa
    27121 agttacgccg cgccccttcc agttgatgca tttgcgtgac atcacgcgcc accatcagca
    27181 actgtttgtg ggtataaggc atgacgcgaa tttccagatg ccgcccggtg ttgagcacca
    27241 gattgagcgg gcgagaaaaa tcacgcgttt tcagatattg cgtaaactcc gggtaacgca
    27301 gtaggttaag gatgttctgc ccgttatctt ccggccagcg caaaccaaga atttgttgcg
    27361 ccagaccgtt acaccagaaa ataccgccct cttccgtggt cagcaccacc gcgtcgggca
    27421 gcgactccgc gccgctacga aagcgtttaa tcagattgcc cagttcacgg cggcgttttt
    27481 tatttcgcag ctgcatctgg tgtaagccgt atagtagcgg ttcccagcta ccacgccccg
    27541 gtggcggggt catactgcga tccacccaca gccaccatga aaggcgcaat aaattccaga
    27601 aatgccagat aagcagtcct gttaccgatg ccagcaaaaa ccagggcagg taaccaaaaa
    27661 atgcacccag gatgaaagcc gggaggcagc aaagtagcag ctccagcacc agccttttcc
    27721 acgacagccg ttccagcacg taagatactc cagttaagaa atcataagcc ctgctctgcg
    27781 tccgatgagc aaggcgttaa aagcgggttg aaaaacgata tcctgtaccg cgcacggtct
    27841 gcaccatgcg gtcatgcccg ccgggctcca gtgctttacg caggcgacga atgtggacat
    27901 cgaccgtgcg gtcttccaca tacacgttag ttccccagac gtggtttaac agctgctcgc
    27961 ggctgtacac gcgctcagga tgcgtcataa aaaagtgcag cagtttaaat tctgtcggcc
    28021 ccatctccag cggctcttcg cccgccatca ctcggtgaga tgtcgggttg agacttaatc
    28081 cctgcatctt aatcacctct tccaccgcca ttggcgaaat acggcgcatt accgctttga
    28141 ttcgcgccac cagctccttc ggcgaaaatg gcttggtgat atagtcatcc gcgccggttt
    28201 caaggccgcg cacgcgatct tcttcttccc ctctggcggt caacatcacc actggaatat
    28261 cccgggtcat cgactcgcgc ttgaggtgtt tgatgaactg gataccggag ccgccaggta
    28321 acatccagtc aaggagaatt aaatccggcc agggttcatt cagttgattc acagcactgt
    28381 cataatcttc cgcttcgacc ggctgaaagc cattttgttc gagcacgaag cagaccattt
    28441 cgcgaattgg agcttcatct tctacgacca gaatacgtct cgccatgatt tgccctgttg
    28501 taataaatag gttgcgatca ttaatgcgac gtcattatgc gtcagattta tgacagattt
    28561 atgaaaagct cgtcgcacat atcttcaggt tattgatttc cgtggcgcag aaaaaagcaa
    28621 atggcacatc tgtttgggta taatcgcgac catgcttttt cgccagggaa ccgttatgcg
    28681 catccttcac acctcagact ggcatctcgg ccagaacttc tacagtaaaa gccgcgaagc
    28741 tgaacatcag gcttttcttg actggctgct ggagacagca caaacccatc aggtggatgc
    28801 gattattgtt gctggtgatg ttttcgatac cggctcgccg cccagttacg cccgcacgtt
    28861 atacaaccgt tttgttgtca atttacagca aactggctgt catctggtgg tactggcagg
    28921 aaaccatgac tcggtcgcca cgctgaatga atcgcgcgat atcatggcgt tcctcaatac
    28981 aaccgtggtc gccagcgccg gacatgcgcc gcaaatcttg cctcgtcgcg acgggacgcc
    29041 aggcgcagtg ctgtgcccca ttccgttttt acgcccgcgt gacattattt ccagccaggc
    29101 ggggcttaac ggtattgaaa aacagcagca tttactggca gcgattaccg attattacca
    29161 acaacactat gccgatgcct gcaaactgcg cggcgatcag cctctgccca tcatcgccac
    29221 gggacattta acgaccgtgg gttccagtaa aagtgacgcc gtgcgtgaca tttatattgg
    29281 cacgctggac gcgtttccgg cacaaaactt tccaccagcc gactacatcg cgctcgggca
    29341 tattcaccgc gcacagatta ttggcggcat ggaacatgtt cgctattgcg gttcccccat
    29401 tccactgagt tttgatgaat gcggtaagag taaatatgtc catctggtga cattttcaaa
    29461 cggcaaatta gagagcgtgg aaaacctgaa cgtaccggta actcaaccca tggcggtgct
    29521 gaaaggcgat ctggcgtcga ttaccgcaca gctggaacag tggcgcgatg tatcgcagga
    29581 gccacctgtc tggctggata tcgaaatcac tactgatgag tatctgcatg atattcagcg
    29641 caaaatccag gcattaaccg aatcattgcc tgtcgaagta ttgctggtac gtcggagtcg
    29701 tgaacagcgc gagcgtgtgt tagccagcca acagcgtgaa accctcagcg aactcagcgt
    29761 cgaagaggtg ttcaatcgcc gtctggcact ggaagacctg gatgaatcgc agcagcaacg
    29821 tctgcagcat cttttcacca cgacgttgcg taccctcgcc ggagaacacg aagcatgaaa
    29881 attctcagcc tgcgcctgaa aaacctgaac tcattaaaag gcgaatggaa gattgatttc
    29941 acccgcgagc cgttcgccag caacgggctg tttgccatta ccggcccaac cggtgcgggg
    30001 aaaaccaccc tgctggacgc catttgtctg gcgctgtatc acgaaactcc gcgtctctct
    30061 aacgtttcac aatcgcaaaa tgatctcatg acccgcgata ccgctgaatg tctggcggag
    30121 gtggagtttg aagtgaaagg tgaagcgtat cgcgcgttct ggagccagaa tcgggcgcgt
    30181 aaccagccgg acggtaattt gcaggtgcct cgcgtagagc tggcgcgctg cgccgacggc
    30241 aaaattctcg ccgacaaagt gaaagataag ctggaactga cagcgacgtt aaccgggctg
    30301 gattacgggc gcttcacccg ttcgatgctg ctttcgcagg ggcaatttgc tgccttcctg
    30361 aatgccaaac ccaaagaacg cgcggaattg ctcgaggagt taaccggcac tgaaatctac
    30421 gggcaaatct cggcgatggt ttttgagcag cacaaatcgg cccgcactga gctggagaag
    30481 ctgcaagcgc aggccagcgg cgtcacgttg ctcacgccgg aacaagtgca atcgctgaca
    30541 gcgagtttgc aggtacttac tgacgaagaa aaacagttaa ttaccgcgca gcagcaagaa
    30601 caacaatcgc taaactggtt aacgcgtcag gacgaattgc agcaagaagc cagccgccgt
    30661 caacaggcct tgcaacaggc gttagccgaa gaagaaaaag cgcaacctca actggcggcg
    30721 cttagtctgg cacaaccggc acgaaatctt cgtccacact gggaacgcat cgcagaacac
    30781 agcgcggcgc tggcgcatat tcgccagcag attgaagaag taaatactcg cttacagagc
    30841 acaatggcgc ttcgcgcgag cattcgccac cacgcggcga agcagtcagc agaattacag
    30901 cagcagcaac aaagcctgaa tacctggtta caggaacacg accgcttccg tcagtggaac
    30961 aacgaactgg cgggttggcg tgcgcagttc tcccaacaaa ccagcgatcg cgagcatctg
    31021 cggcaatggc agcaacagtt aacccatgct gagcaaaaac ttaatgcgct tgcggcgatc
    31081 acgttgacgt taaccgccga tgaagttgct accgccctgg cgcaacatgc cgagcaacgc
    31141 ccactgcgtc agcgcctggt cgcgctgcat gggcagattg ttccccaaca aaaacgtctg
    31201 gcgcagttaa tggtcactat ccagaatgtc actctggaac agacgcaacg taatgtcgca
    31261 cttaacgaaa tgcgccagcg ttataaagaa aagacgcagc aacttgccga tgtgaaaacc
    31321 atttgcgagc aggaagcgcg catcaaaacg ctggaagccc agcgtgcaca gttacaggcg
    31381 ggtcagcctt gtccactttg tggttccacc agccacccgg cggtcgaggc gtatcaggcg
    31441 ctggagcctg gtgttaatca gtctcgatta ctggcgctgg aaaacgaagt taaaaagctc
    31501 ggcgaagaag gtgcggcgct gcgcgggcaa ctggatgcat taacgaagca gcttcagcgc
    31561 gatgaaaacg aagcgcaaag cctccgacaa gatgagcaag cacttactca acaatggcaa
    31621 gccgtcacgg ccagcctcaa tatcaccttg cagccacagg acgatattca accgtggctg
    31681 gatgcacaag atgagcacga acgccagctg cggttactca gccaacgaca tgaattacaa
    31741 gggcagattg ccgcgcataa tcagcaaatt atccagtatc aacagcaaat tgaacaacgt
    31801 cagcagcagc tcttaacggc attggcgggt tatgcactga cattgccaca ggaagatgaa
    31861 gaagagagct ggttggcgac acgtcagcaa gaagcgcaga gctggcagca acgccagaac
    31921 gaattaaccg cgctgcaaaa ccgtattcag cagctgacac cgattctgga aacgttgccg
    31981 caaagtgatg atctcccgca cagcgaagaa actgtggcgc tggataactg gcggcaggtt
    32041 catgaacaat gtctcgcatt acacagccag cagcagacgt tacagcaaca ggatgttctg
    32101 gcggcgcaaa gtctgcaaaa agcccaagcg cagtttgaca ccgcgctaca ggccagcgtc
    32161 tttgacgatc agcaggcgtt ccttgcggcg ctaatggatg aacaaacact aacgcagctg
    32221 gaacagctca agcagaatct ggaaaaccag cgccgtcagg cgcaaacgct ggtcactcag
    32281 acagcagaaa cgctggcaca gcatcaacaa caccgacctg acgggttggc tctcactgtg
    32341 acggtggagc agattcagca agagttagcg caaactcacc aaaagttgcg tgaaaacacc
    32401 acgagtcaag gcgagattcg ccagcagctg aagcaggatg cagataaccg tcagcaacaa
    32461 caaaccttaa tgcagcaaat tgctcaaatg acgcagcagg ttgaggactg gggatatctg
    32521 aattcgctaa taggttccaa agagggcgat aaattccgca agtttgccca ggggctgacg
    32581 ctggataatt tagtccatct cgctaatcag caacttaccc ggctgcacgt gcgctatctg
    32641 ttacagcgca aagccagcga ggcgctggaa gtcgaggttg ttgatacctg gcaggcagat
    32701 gcggtacgcg atacccgtac cctttccggc ggcgaaagtt tcctcgttag tctggcgctg
    32761 gcgctggcgc tttcggatct ggtcagccat aaaacacgta ttgactcgct gttccttgat
    32821 gaaggttttg gcacgctgga tagcgaaacg ctggataccg cccttgatgc gctggatgcc
    32881 ctgaacgcca gtggcaaaac catcggtgtg attagccacg tagaagcgat gaaagagcgt
    32941 attccggtgc agatcaaagt gaaaaagatc aacggcctgg gctacagcaa actggaaagt
    33001 acgtttgcag tgaaataact attcagcagg ataatgaata cagaggggtg aattatctct
    33061 tggccttgct ggtcgttatc ctgcaagcta tcactttatt ggctacggtg attggtagcc
    33121 gttctggtgg ttgtgatggt ggtatgaaaa aagtcatttt atctttggct ctgggcacgt
    33181 ttggtttggg gatggccgaa tttggcatta tgagcgtgct cacggagctg gcgcataacg
    33241 taggaatttc gattcctgct gccgggcata tgatctcgta ttatgcactg gtggtggtgg
    33301 tcggtgcgcc aatcatcgca ctcttttcca gccgctactc actcaaacat atcttgttgt
    33361 ttctggtggc gttgtgcgtc attggcaacg ccatgttcac gctctcttcg tcttacctga
    33421 tgctcgccat tggtcggctg gtatccggct ttccgcatgg cgcatttttt ggcgtcggag
    33481 cgatcgtgtt atcaaaaatt atcaaacccg gaaaagtcac cgccgccgtg gcggggatgg
    33541 tttccgggat gacagtcgcc aatttgctgg gcattccgct gggaacgtat ttaagtcagg
    33601 aatgctggcg ttacaccttt ttattgatcg ctgtttttaa tattgcggtg atggcatcgg
    33661 tctatttttg ggtgccagat attcgcgacg aggcgaaagg aaatctgcgc gaacaatttc
    33721 actttttgcg cagcccggcc ccgtggttaa ttttcgccgc cacgatgttt ggcaacgcag
    33781 gtgtgtttgc ctggttcagc tacgtaaagc catacatgat gtttatttcc ggtttttcgg
    33841 aaacggcgat gacctttatt atgatgttag ttgggctagg gatggtgctg ggaaatatgc
    33901 taagtggcag gatttcagga cgttattcac cactgcgcat tgcagcagtg actgacttta
    33961 tcattgtact ggcactgctg atgctctttt tctgcggcgg catgaaaaca acgtcgctta
    34021 tttttgcttt tatttgttgc gcgggattat ttgccctttc agcacctctg caaatattgt
    34081 tactgcaaaa tgccaaagga ggagagttat taggtgccgc aggtgggcaa atagcgttta
    34141 acctcggtag cgccgtcggc gcatattgcg gaggtatgat gctgacgctg gggctggcat
    34201 ataattacgt ggcgctgcct gccgccctgc tttcgtttgc tgcgatgtca tcgttgctgc
    34261 tgtatggtcg ctataagcgc cagcaagcgg cggatagtcc ggtgctggcg aaaccactgg
    34321 ggtagattac tgtcacggtg cgcgttgctt attgccggat gcggcgtgaa cgccttatcc
    34381 gccctacgcg gttctggcac attttgtagg cctgataaga cgcggcaagc gtcgcatcag
    34441 gcatgatgcg ccaattgcct acgtttttta ctcttgtggc cataaccacg cagcgccgcg
    34501 tacgccgctg gaatcaccgt gcttcgcctt actcaccggc gtttcacatt cgccgccgaa
    34561 gacaaattgt ttaatcaact gcccaaccgt ttgatataaa cggtctacat tgctcatccc
    34621 gccccccagg acaatcacat ccggatcgag aatattcacg acatgtgcca gcgattttgc
    34681 cagccgcagc tcgtagcgac gcaatgccag ttccgctacc ggatcgcttt cttcaaccag
    34741 gcggataatt tcactgcctt tcagcgcatg tccgctcaaa cgacgataat ccgtcgcgaa
    34801 tcccgtgccc gaaataaagg tttcaataca accttgttta ccgcaataac aagggacttc
    34861 ctcgcgataa cgcagttcgt cttcgtccat ccacggtagc ggattgtgtc cccactcacc
    34921 tgccgtgcca ttgccgccga tatgcgtccg cccattgaat gccacgcccg cgccgcatcc
    34981 cgtgccgata atcacggcaa ataccgtctg cgctcccgct gccgcgccat ctactgcttc
    35041 tgaaaccgcc agacagttgg catcatttgc cagccgcact tcccgctgca acctcgcgct
    35101 taagtcttta tcgaatggct gaccgttgag ccaggttgaa ttgacattct tcaccacacc
    35161 ggtgtagggc gaaatcgaac ctggaatgcc catacctacc gttccgcgct gccctgtcgc
    35221 ctgctccgcc atatcaacca acgtggcgat cgtttcaata gtctgccggt aatcatcacg
    35281 cggcgtgggc agacgatggc ggtacaactg ctcccctgca tcgcccagtg caatcacttc
    35341 agttttggtg ccgcctaaat cgatacctat acgcactgta ctctccttat ttttttcaat
    35401 atcaatagcg tagagacgga caaccggatt ggcaatgcaa ggccgccgac aattcgttat
    35461 catgcccgct aaatttaacg acaaggccgt ggaaattatc atgctgtggt tcaaaaattt
    35521 aatggtttac cgtcttagcc gcgagatttc gctgcgtgca gaagagatgg aaaaacagct
    35581 agcctcgatg gcatttaccc catgcggcag ccaggacatg gcgaagatgg gctgggttcc
    35641 tccgatggga tcgcacagcg atgcgttaac gcacgttgcc aatggtcaaa ttgttatctg
    35701 cgcgcgcaaa gaagaaaaaa tcctcccgtc tccggtgatt aaacaggcgc tggaagcgaa
    35761 aatcgccaaa ctggaagcgg aacaggcgcg taagctgaag aaaaccgaaa aagattcgct
    35821 gaaagacgaa gtgctgcact ctctgctgcc gcgtgctttc agccgtttta gccagacaat
    35881 gatgtggctc gacacggtta acggtttgat tatggtggac tgcgccagtg ccaaaaaagc
    35941 ggaagatacg ctggcattac tgcgtaaaag cctggggtcg ttaccggttg taccattgag
    36001 catggaaaac ccgattgaac tgacgctgac cgaatgggtt cgctccggta gtgcggcaca
    36061 gggcttccag ctgcttgatg aagccgagct gaaatcgttg ctggaagatg gcggcgtgat
    36121 ccgcgcgaag aaacaagatc tgaccagcga agagatcacc aatcacattg aagccggaaa
    36181 agtggtgact aaactggcgc tcgactggca gcagcgcatt cagtttgtga tgtgcgacga
    36241 tggttcgctc aagcgtctta agttctgcga cgagctgcgc gatcaaaacg aagatatcga
    36301 ccgtgaagat ttcgcccagc gttttgatgc cgatttcatc ctgatgactg gtgaactggc
    36361 agcgttaatt caaaacctga ttgaaggatt aggtggcgaa gcacaacgtt aattgctgat
    36421 tttcctttaa tgccggatgc gacgcctgcc gcgtcttatc cggcgtacga agccacacca
    36481 ggcatataat tattcgctac ggcgagcaat aatttttagc gcagcaatat tatgcgtttt
    36541 acgccgtaac tgatctaatc gcctcatccc tattcgcctg tcagcagcac gcagaatatt
    36601 cactaacccc catttatcgc tggtaaattg ttttgttaac agctccgctt ttggggtatc
    36661 aatatattca cgcaataaat cagaaattga acaaatatcc gtcaggtaag gataccagca
    36721 agtttcaccg tgaaaattac gcactccagc gttacctgca ataaagtctt tcgggtaatc
    36781 ccagatcacg tttttatcca acgtgatcca ataacgcggt aaatcggtac ttccaccatt
    36841 ttggctgcgc atgggataac gggtacagtg aatttgcagg ttgatcgttg gcgtcagtaa
    36901 gtcatagatc tcccgctgta acttgctcca tggacgttgt gtcattgttt ttcctcaagc
    36961 cggtgacgcg cttcactgcg aattttccgc gttgccccgg ttttcactaa atcacgtaac
    37021 tgcgaaagat cgggttgttc gacaaataag agtaatgatg gatcctcttt taaccacgcc
    37081 gttttcacat cggcagccag ctgtggatta ttaatagcca gcgaccgatc ctgaggctca
    37141 gtcaatgtcg ttaacaacga cgccggggta tgtgcgttgc gtaatgccgt ttggcgcaaa
    37201 tgatcattgc gactttgcag caatgcacgc caccattcat catctgattt atcttttatc
    37261 gtagccagtt ccgcaaaaaa tacctggaga tcttcactga tatttatatc aaccttgatg
    37321 aattatgaat atatcggaaa ttgaaaaaat cgcagcaaag gaaatattat tatgggttaa
    37381 agagaggata tttcagaaaa atgccctccc gttcaagggg aaggcgagga attacagata
    37441 gcggcacaga taagaggtag gttcggcaac ttgaagatga aactcactgt gaccgggaac
    37501 attaaacacc gaaccggctt catacacttg ccagtcggtc gcgtcaggca gtaacacatt
    37561 cagcgcgcca ctgattaccg tcatctcttc cggctcagcg gtgctgaagg tgtattcgcc
    37621 ttcaaccata acacccacgc tggcgcgacc agtgctgctg ctggaaaagc cgatcgattt
    37681 cactttgccg gaaaagtact cattactttg aagcataaac tggccctgtc tggtagttga
    37741 acatctactt cactataggg gccagaggcg ctggctgtca cgcaaaatta caccagtaat
    37801 tccgcagcca gccgtgcaat caatacgtta gagagcaaga caggaacatc gagctgtttt
    37861 tgcagtaaat cgcgatgacg ttggtgaaat cctaaacaat ccagcatgat gacatcagcc
    37921 ccttttgcca gtaattcttt cccagcatca atgatttttt gttctgaatc atgaatgggg
    37981 ttacccaatg aaaataccgg cgatttctgc aaaatttgcc atttttgcgc ctgaacgggc
    38041 agcatctcct caaccggaac gataaccccc acctgatgat cttcaacaat agaggaaacc
    38101 agtggaggca atattcgcga cggctcaaga aagatcgtat tacgcgcagt catactacta
    38161 atgtttgctg tactcattaa taaaatgacg tcataaccct gattatcgag cacttcaacc
    38221 acaccttgca gatctcgctc cactttacga cgcgaaacat gggccagctg gttgtcattt
    38281 aataatgtga gaatggtgtc ttcgcctgct tctggcgcgt actccgccat cacttcttca
    38341 cgacttaact tccccagcag gctatgatgg gaaatattat cttcgtcaat gtattccgtc
    38401 aggagcggca aaacctcctg cataggtaca atgccgatgg tcaggatcgc caacgacgca
    38461 ctcattgttc atccacctta cttttctttc gtcgttaatt accgggcaag tgtgaagcac
    38521 cactgctgac acttacttgt gctacaaatg acaaaaagcg tagcagcaga cgtgcacggc
    38581 ataatgtaaa gattccaaat gattccagta atggaatgtt attgtttaat attctaatta
    38641 ttagaaaaac ataaattatg aaaaatgtga cgcagatccc ttgtagtggc actcagaatc
    38701 ccttccgagc agtctgatgc ggtcactctt tgtcagggtc ctcatagcgt tttttcgcat
    38761 ccatcgcttc ctgagccgtc ggatgttcac tgatcaacga gtctggttta ggatgatcgg
    38821 ctctgagttg gtaccaggtt accgtctgtc ctggctttcc tttttcaatc gtcactatat
    38881 atgcttcacg aggataaggc ggtttcgttg gcatcgttct tccttatttc acgggatgaa
    38941 cgttaagtat aggcgctcta aaatcaacaa ttgatcgtct gtgccagggc gctacgaatt
    39001 tcagaaatca cctggctggg ttcgtttgtt gcgtcgatga taatatgcgc aacttcgcga
    39061 tatagcgcat cgcgttcttc cagcacttcc tgaacttctt cgctcagcgg ttttcccgtt
    39121 aaggttggcc gtaaatcttc ttccggtgca gcttgcagtc ggttaaccag gactgatact
    39181 ggcgcacaca aataaaccac gatcccgtta ttttgcatga agtgacgatt aaattccgtc
    39241 agaataatgc cgccgcctgt agcgataacg gtggatgccg cagttaccgc ttccagcgcc
    39301 gccgtttctc tggcgcgaaa tcccgcccac tcttcccttt cgacgatctc cgcgaccgtc
    39361 atattgagct gtgattgcaa ccactgatcg gtatcgacaa accgacggtt aagcgaatcg
    39421 gcaagggcca ttccgaccgt tgttttacca cagccccgag gcccgatcag aaaaagaggt
    39481 tgtgtcatcg tgggttttcc ccaataggtc gcaatgcggc gaaagccggt gtcatgagaa
    39541 tagcgatcat accatcaaac tagtacaatt tcgattgtaa
//
LOCUS       DAZPCS010000023        37773 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZPCS010000023 DAZPCS010000000
VERSION     DAZPCS010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37773
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.023"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..73)
                     /locus_tag="ACHUQ7_001154"
     CDS             complement(<1..73)
                     /locus_tag="ACHUQ7_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="MliC; membrane-bound lysozyme inhibitor of c-type
                     lysozyme; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor"
                     /protein_id="HFR1134662.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQL"
     gene            complement(171..1280)
                     /gene="anmK"
                     /locus_tag="ACHUQ7_001155"
     CDS             complement(171..1280)
                     /gene="anmK"
                     /locus_tag="ACHUQ7_001155"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFR1134663.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQLVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAVLLLGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            1554..2021
                     /gene="slyB"
                     /locus_tag="ACHUQ7_001156"
     CDS             1554..2021
                     /gene="slyB"
                     /locus_tag="ACHUQ7_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFR1134664.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(2068..2502)
                     /gene="slyA"
                     /locus_tag="ACHUQ7_001157"
     CDS             complement(2068..2502)
                     /gene="slyA"
                     /locus_tag="ACHUQ7_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFR1134665.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            2703..2939
                     /locus_tag="ACHUQ7_001158"
     CDS             2703..2939
                     /locus_tag="ACHUQ7_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFR1134666.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            2942..3799
                     /locus_tag="ACHUQ7_001159"
     CDS             2942..3799
                     /locus_tag="ACHUQ7_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFR1134667.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGELLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFIFAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQLVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            3799..5811
                     /locus_tag="ACHUQ7_001160"
                     /pseudo
     CDS             3799..5811
                     /locus_tag="ACHUQ7_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(5812..6333)
                     /gene="sodC"
                     /locus_tag="ACHUQ7_001161"
     CDS             complement(5812..6333)
                     /gene="sodC"
                     /locus_tag="ACHUQ7_001161"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFR1134668.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(6414..7310)
                     /locus_tag="ACHUQ7_001162"
     CDS             complement(6414..7310)
                     /locus_tag="ACHUQ7_001162"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFR1134669.1"
                     /translation="MVQRITIAPEGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(7359..7598)
                     /locus_tag="ACHUQ7_001163"
     CDS             complement(7359..7598)
                     /locus_tag="ACHUQ7_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFR1134670.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            7701..8300
                     /gene="nemR"
                     /locus_tag="ACHUQ7_001164"
     CDS             7701..8300
                     /gene="nemR"
                     /locus_tag="ACHUQ7_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFR1134671.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLAELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            8337..9434
                     /gene="nemA"
                     /locus_tag="ACHUQ7_001165"
     CDS             8337..9434
                     /gene="nemA"
                     /locus_tag="ACHUQ7_001165"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFR1134672.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAAFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQLKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            9515..9922
                     /gene="gloA"
                     /locus_tag="ACHUQ7_001166"
     CDS             9515..9922
                     /gene="gloA"
                     /locus_tag="ACHUQ7_001166"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFR1134673.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            10025..10672
                     /gene="rnt"
                     /locus_tag="ACHUQ7_001167"
     CDS             10025..10672
                     /gene="rnt"
                     /locus_tag="ACHUQ7_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFR1134674.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            10765..15223
                     /locus_tag="ACHUQ7_001168"
                     /pseudo
     CDS             10765..15223
                     /locus_tag="ACHUQ7_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(15267..16475)
                     /locus_tag="ACHUQ7_001169"
     CDS             complement(15267..16475)
                     /locus_tag="ACHUQ7_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFR1134675.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFQKRAAFPR
                     FKKRGQNAAFRYPQDVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPVHPSASMIGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWSGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQ
                     EPTEMIQATA"
     gene            16498..16908
                     /gene="tnpA"
                     /locus_tag="ACHUQ7_001170"
                     /pseudo
     CDS             16498..16908
                     /gene="tnpA"
                     /locus_tag="ACHUQ7_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278583.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(17022..17369)
                     /gene="grxD"
                     /locus_tag="ACHUQ7_001171"
     CDS             complement(17022..17369)
                     /gene="grxD"
                     /locus_tag="ACHUQ7_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFR1134676.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            17704..18531
                     /gene="mepH"
                     /locus_tag="ACHUQ7_001172"
     CDS             17704..18531
                     /gene="mepH"
                     /locus_tag="ACHUQ7_001172"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFR1134677.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSMTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            18659..19240
                     /gene="sodB"
                     /locus_tag="ACHUQ7_001173"
     CDS             18659..19240
                     /gene="sodB"
                     /locus_tag="ACHUQ7_001173"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFR1134678.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19386..20555)
                     /locus_tag="ACHUQ7_001174"
     CDS             complement(19386..20555)
                     /locus_tag="ACHUQ7_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1134679.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMHVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVTGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(20721..20810)
                     /gene="cydH"
                     /locus_tag="ACHUQ7_001175"
                     /gene_synonym="ynhF"
     CDS             complement(20721..20810)
                     /gene="cydH"
                     /locus_tag="ACHUQ7_001175"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFR1134680.1"
                     /translation="MSTDLKFSLVTTVIVLGLIVAVGLTAALH"
     gene            21109..22134
                     /gene="purR"
                     /locus_tag="ACHUQ7_001176"
     CDS             21109..22134
                     /gene="purR"
                     /locus_tag="ACHUQ7_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFR1134681.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(22131..23063)
                     /gene="punR"
                     /locus_tag="ACHUQ7_001177"
     CDS             complement(22131..23063)
                     /gene="punR"
                     /locus_tag="ACHUQ7_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFR1134682.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLSDSETLNKEWLREPEETPATGD"
     gene            23176..24387
                     /gene="punC"
                     /locus_tag="ACHUQ7_001178"
     CDS             23176..24387
                     /gene="punC"
                     /locus_tag="ACHUQ7_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFR1134683.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            24678..25826
                     /gene="cfa"
                     /locus_tag="ACHUQ7_001179"
     CDS             24678..25826
                     /gene="cfa"
                     /locus_tag="ACHUQ7_001179"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFR1134684.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCKGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(25866..26507)
                     /gene="ribE"
                     /locus_tag="ACHUQ7_001180"
     CDS             complement(25866..26507)
                     /gene="ribE"
                     /locus_tag="ACHUQ7_001180"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFR1134685.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            26722..28095
                     /gene="mdtK"
                     /locus_tag="ACHUQ7_001181"
     CDS             26722..28095
                     /gene="mdtK"
                     /locus_tag="ACHUQ7_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFR1134686.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRYMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPEPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(28136..29392)
                     /locus_tag="ACHUQ7_001182"
     CDS             complement(28136..29392)
                     /locus_tag="ACHUQ7_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFR1134687.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVLGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            29700..29776
                     /locus_tag="ACHUQ7_001183"
     tRNA            29700..29776
                     /locus_tag="ACHUQ7_001183"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29734..29736,aa:Val,seq:gac)
     gene            29781..29857
                     /locus_tag="ACHUQ7_001184"
     tRNA            29781..29857
                     /locus_tag="ACHUQ7_001184"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29815..29817,aa:Val,seq:gac)
     gene            29965..30270
                     /locus_tag="ACHUQ7_001185"
     CDS             29965..30270
                     /locus_tag="ACHUQ7_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFR1134688.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            30396..31818
                     /locus_tag="ACHUQ7_001186"
                     /pseudo
     CDS             30396..31818
                     /locus_tag="ACHUQ7_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
     gene            complement(31830..32642)
                     /gene="ydhT"
                     /locus_tag="ACHUQ7_001187"
     CDS             complement(31830..32642)
                     /gene="ydhT"
                     /locus_tag="ACHUQ7_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFR1134689.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGLGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(32646..33431)
                     /gene="phsC"
                     /locus_tag="ACHUQ7_001188"
     CDS             complement(32646..33431)
                     /gene="phsC"
                     /locus_tag="ACHUQ7_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFR1134690.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(33428..34096)
                     /locus_tag="ACHUQ7_001189"
     CDS             complement(33428..34096)
                     /locus_tag="ACHUQ7_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFR1134691.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYLVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(34160..34798)
                     /locus_tag="ACHUQ7_001190"
     CDS             complement(34160..34798)
                     /locus_tag="ACHUQ7_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFR1134692.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(34811..36912)
                     /locus_tag="ACHUQ7_001191"
                     /pseudo
     CDS             complement(34811..36912)
                     /locus_tag="ACHUQ7_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
     gene            complement(36933..37559)
                     /locus_tag="ACHUQ7_001192"
     CDS             complement(36933..37559)
                     /locus_tag="ACHUQ7_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFR1134693.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
BASE COUNT         9061 a         9889 c         9293 g         9530 t
ORIGIN      
        1 ccagttgatt aaaggcgcta caaccggaga gaaggaccgg caaaatgata attaacagtt
       61 ttttcatagt catatcccga agactttcct ggtccggagg gcaaataccc tccttaatct
      121 tccaagtgta acggcagaca cgataagaaa aaattcagtt aattctgata tcacgagttg
      181 gcggggaaaa tagcccccag taccgtctcc tggcttgcgc cagtgacgga aggcagattt
      241 cctggtaatc ccgccagcgt ccgccaggca agccaggcga aagccaatgc ttccatgtca
      301 tcgccactaa tgccaacggc atcggtggtg gtgacttctg tgcctagcag taataccgcc
      361 agacgcgcca tgagtagcgg attacgacta cctccaccac ataccatcaa tcgttcgcag
      421 ccaccgctca acaaaacttg ttcagaaatg gtcacggcgg tgagttctgc cagtgtggcc
      481 tgaacatcac ggggatcaac acccggaaaa tggcgtaaat ggcgctccag ccagccgtag
      541 ttaaagtatt cgcgtccggt gcttttcggt gcaggttgcg agaaatacgg gtcgctgagc
      601 atattttgca gcagtgggag aataacttta cccgcccgtg cccactcggc atctttatcg
      661 taaggtttac ctgcctgacg ccagatccag gcatccatca gcatgttacc aggaccggta
      721 tcgtagccgc caaccagctg cccaggaatg agcagtgaca gattggcgat gccgccaata
      781 ttgagcacca ttcgtcgctc ggttgggtga gccagcaagg catgatggaa cgcaggtacc
      841 agcggtgcgc cttgtcctcc caaggcaata tcgcgacggc gaaaatcacc gacaaccgta
      901 attccggtgc gtgccacaat ttgattgtta tcgccaatct gtaaagtgtg tggtgccacg
      961 cccgtcggtt catgccagac ggtttgaccg tgacagccga tcgcaactat atctctcgcc
     1021 tgcaggtttt gttccttaag caaggcattg accgcatcag caaaaagtcg cccgagttga
     1081 gtatcaagct gtccaaactg cgaaagtgta agctgctggc cctggcaaat atccagtaca
     1141 gcctgtttca gagacaccgg gatcggccag ctcaagcttg ccagctgtgc gacccggtgt
     1201 tcatcaattg tcgccaacac aacatcaaca ccatcaaggc tggtgcctga cataacgcca
     1261 ataaagcggc ccgatttcat agttcatcct ttttcaatct gacgtttgcg caccactcaa
     1321 acataaactt ttcgtgaata ccatgcggaa tgaccgattt ttaccgttgg tagtaaaaca
     1381 ttatcttcaa atcaataatc atcatgaatg ttttgtttat aattggttga tcctactttc
     1441 attatgattt gctcatattt ggtagaacat gtaaccatgg attcacatat gccatatact
     1501 ttgaccatga gggatgcttg cgtggcgttt catggtgaac aggagatttt tcaatgatta
     1561 aacgcgtatt ggttgtttca atggtaggtc tgtctcttgt cggttgtgtt aataacgaca
     1621 ccctgtcagg ggatgtttat accgcttctg aagcgaaaca agtacagaat gtcagctatg
     1681 gcaccatcgt taacgtacgt ccggtacaga ttcagggcgg tgatgattcc aacgttatcg
     1741 gtgcaattgg cggtgctgtt cttggtggtt tcctggggaa tactgttggt ggcggaaccg
     1801 ggcgttctct ggctactgca gcaggcgctg ttgcaggtgg cgtagctggt cagggcgtac
     1861 agagtgcaat gaacaaaacg cagggtgtcg agctggaaat tcgtaaagac gatggtaata
     1921 ccatcatggt ggtacagaaa caaggcaaca ctcgtttctc tccgggccaa cgtgtcgtac
     1981 tggccagcaa tggcagtcag gtgaccgttt ctccgcgcta aataagtttg cgtgtggtca
     2041 ggttactgac cacacgcccc cttcatttca ccctttggcc tgtaactcaa tgatattatg
     2101 ctcaagtttt gcgatgagcg taatcagttg ctccagttcc tctgcggaga tgccatgtaa
     2161 tatttccgcg cgggttttgt taataacagc ttccatttcg ctgatcagtg gctctgcctt
     2221 ttccgtcagt ttaatacgtt tagcccgacg atcgctggca caagtttgac gcgaaattaa
     2281 ccctttttct tccagttggt ccagagtacg gaccagtgat ggctgctcga tgccaatcgc
     2341 ttttgccagt tgaatttgcg actggtctgg aggtaactga tggatattgt gtaacgtaac
     2401 ccaatgggtt tgtgttaact ccagcggttt caggcgatgg tctatcagag cacgccatat
     2461 gcgcaccaac cgtgccagat cagaacctag tggcgattcc aatttcatct ccttataatt
     2521 agcttgctaa gatattatgc ggcttttaga atagtgtgca gcaattgtat tgctaaaaca
     2581 aatgtattgc tgcatttggt taccgtcaga catatttttc agaaattgcg cgtaaatttt
     2641 tcgcacttaa agaatattta ttaatctaac gcaatatatt cggtcgtaaa ggaatctact
     2701 ttgtgaagtt tatgctcaat gcaacaggat tgcccttaca agatctggtg ttcggtgcgt
     2761 ccgtctattt tcctccgttt ttcaaagcat tcgcgtttgg attcgtcatc tggcttgtcg
     2821 tacaccgcct gcttcgtggc tggatctacg ccggcgatat ctggcatccc ttgttaatgg
     2881 atttatcgct gtttgcgatt tgcgtttgcc ttgctctggc aatactgatt gcgtggtaac
     2941 tatgtcaatt aaaacaatta agtatttctc aacaatcatt gtagcgatag ttgcggttct
     3001 tgccggatgg tggctgtgga attattacat gcaatctccg tggacacgcg acgggaaaat
     3061 acgcgcagaa caggtttcta tcaccccaca ggtgtcggga cgtattgttg agctgaatat
     3121 aaaagacaac caactggtta acgcggggga acttctgctc acgatcgata aaacgccctt
     3181 tcagatcgcc gagctgaacg cacaggctca gctggcgaaa gcgcaatctg acctcgcaaa
     3241 agccaataac gaagcgaatc gtcgtcgtca tctctcacaa aattttattt ttgccgaaga
     3301 actcgatacc gctaacctca atgttaaagc gatgcaggcc agtgttaatg ccgcacaggc
     3361 gacgctgaaa cagacacaat ggcaactggc gcaaacggaa attcgcgccc cggtgagtgg
     3421 atgggtgact aacctcacta cccgcatcgg cgattacgct gacaccggga aaccgctgtt
     3481 tgcccttgtc gacagccact cgttttacgt cattggttat tttgaagaaa ccaagttgcg
     3541 ccatatccgc gaaggtgcac cagcacaaat taccctctat agtgataaca aaacgttaca
     3601 gggtcacgtt tccagtatcg gtcgggcgat ttatgatcag agcgttgaaa gtgactcaag
     3661 ccttattccg gatgtgaaac ctaacgtccc ctgggttcgt ctcgcccaac tcgttcccgt
     3721 ccgctttgcg ctggataaag ttccaggcga tgtcacgctg gtgtcgggca ccacctgtag
     3781 cattgccgta ggtcaataat gaacgcatcg tcatggtcct tgcgcaattt gccctggttc
     3841 agggccacgc tggcgcaatg gcgttatgcg ttacgcaata ccattgccat gtgtctggcg
     3901 ctgacggttg cctattattt gaatctggat gaaccctatt gggcgatgac ctcggctgca
     3961 gtggttagct ttcccaccgt tggcggtgtc atcagcaaaa gcctcggacg catggctggc
     4021 agtttactgg gagccattgc ggcactgctt cttgccggac atacgctcaa tgagccgtgg
     4081 ttttttctat tgagcatgtc ggcgtggctt ggcttttgta cctaggcctg tgcgcacttc
     4141 acgaataacg tcgcgtatgc atttcaactg gcgggctaca cggctgccat catcgccttt
     4201 ccgatggtta atattactga ggccagccag ctgtgggata tcgctcaggc gcgcgtttgc
     4261 gaggtgatag tcggtatttt gtgcggcggc atgatgatga tgatcctgcc tagcagttcc
     4321 gatgctactg cccttttaac tgcattgaaa aacatgcacg cccgattact ggaacatgcc
     4381 agtttactct ggcagcctga aacaaacgat gccattcgtg cagcacatga gggggtgatt
     4441 gggcagatac tgaccatgaa tttgctgcgt atccaggctt tctggagcca ctatcgtttt
     4501 cgccagcaaa acgcgcgcct taatgcgcta ctccaccagc aattacgtat gaccagtgtc
     4561 atctccagcc tgcgacgtat gttgctcaac tggccctcac cgccaggtgc cacgcgagaa
     4621 attctcgaac aattgctgac ggcgctcgcc agttcgcaaa cagatgctta caccgtcgca
     4681 cgtattatcg ccccgctacg cccgaccaac gtcgccgact accggcacgt cgccttctgg
     4741 cagcgactac gttatttttg ccgcctttat ctgcaaagta gtcaggaatt acatcgtctg
     4801 caaagcggtg tagatgatca tgccagactc gcacggacat ccgctctggc tcgtcatacc
     4861 gacaacgccg aagctatgtg gagcgggctg cgtacatttt gtacgttgat gatgattggc
     4921 gcatggagta ttgcatcgca atgggatgcc ggtgccaatg cattaacgct ggcagcaatt
     4981 agctgcgtac tctactccgc cgtcgcagca ccgtttaagt cgttgtcact gctgatgcgc
     5041 acgctggtgt tactttcgct attcagcttt gtggtcaaat ttggcctgat ggtccagatt
     5101 agcgatctgt ggcaattttt actgtttctc tttccactgc tggcgacaat gcagcttctt
     5161 aaattgcaga tgcccaaatt tgccgcactg tgggggcaac tgattgtttt tatgggttcg
     5221 tttatcgctg tcactaatcc cccggtgtat gattttgctg attttcttaa ctataatctg
     5281 gcaaaaatcg ttggcgttgc gctggcttgg ctagcgttcg ccattctgcg tccaggatcg
     5341 gatgctcgta aaagccgccg ccatattcgc gcgctgcgcc gggattttgt cgatcagcta
     5401 agccgccatc caacactgag tgaaagcgaa tttgaatcgc tcacttatca tcacgtcagt
     5461 cagttgagta acagccagga tgcgctggct cgccgttggt tattacgctg gggtgtagtg
     5521 ctgctgaact gttctcatgt tgtctggcaa ttgcgcgact gggaatcgcg ttccgatccg
     5581 ttatcgcgag tacgggataa ctgtatttca ctgttgcggg gagtgatgag tgagcgtggc
     5641 gttcagcaaa aatcactggc ggccacactt gaagaattac agcggatttg cgacagcctt
     5701 gcccgtcatc atcaacctgc cgcccgtgag ctggcggcaa ttgtctggcg gctgtactgc
     5761 tcgctttcgc aacttgagca agcaccaccg caaggtacgc tggcctcgta attacttaat
     5821 tacaccacag gcatagcgtt caccgccacc gcccagcggt ttaggttgat cggacatatt
     5881 atcgccgcca acgtggacca tcagcgcctt gtctttgatt tcatccagtg atttcagacg
     5941 aggcgcgatg acggcatcgg tagctttgcc gtcattattg acgaccagtg caggcagatc
     6001 gcctaaatgc ccggtacctt ctggcccttc atgtttaccg gtattttgtg gatcaagatg
     6061 cccgcctgcg gattccgcgg cgctggcttt gccatctttg gtggctggct ggcagcttcc
     6121 tttggcatga atatggaagc catgttcacc ggggggtaat gctttcagat cgggtgaaaa
     6181 ctccagacct ttatcggttt cagtaatggt gacgctacca attgactgcc caaccccttg
     6241 cgacgtgacg aggttcatct cgactttttc actggcagct tgtgcgccgg tcgcaacaac
     6301 cagcgccaga atggccagac taaaacgttt cataggacct ccgttcattg tgcattcctg
     6361 aataagtgta aaccagtgac acatttttga acggcggcta ttcctaaaag tgcttacggt
     6421 acgtcgtacc ccagtgcagc tttacggata cgaaaccatt gttgacgggt cattttcagt
     6481 gtttctgctt cgacagctgc ccgtacgcgc tcaattttac ctgagccgat aattggcagc
     6541 ggctgcgacg gtaaacgtaa tacccaggcg taaaccacct gttcaatcga gcccgcgttt
     6601 aactcctccg ccaccacagc cagttcatca cgcagcggct ggaaataatc atcattaaac
     6661 agacgaccac caccaaggca ggaccacgcc atcggacgaa cacgcagttg ttgtagttgg
     6721 tcgagcgtgc catccagcag taacggctga tgcaccgggg atatttccac ctgattagtg
     6781 gcaagggtaa acggcagacg tgattgcaac agggcaaatt gcgcaggcgt aaagttcgat
     6841 acgccaaaat gacgcacttt gccgctctga tgcagatgtt tgaacgcgtc cgccacttca
     6901 tcggcatcca ttaacgggtc tggtcggtgg attaacagca gatccagatg atcggtcgcg
     6961 agattaatta gcgactgttc ggcgctctta atgatgtgat tgcggtcagt gatgtaatga
     7021 ccaatgacgt tttcttcacg cgcggtcgtc gcgataccgc atttactgac gatttccatc
     7081 cgttcacgca ggtgaggtgc cagtttcagt gcctcgccaa acgccgcttc gcactgatag
     7141 ccaccataaa tatcagcatg gtccacggtg gtcacgccga gatccagatg ctcttcaata
     7201 aaactgacca gctggcgggc ggacatattc cagtccatca atcgccagta gcccatcaca
     7261 aaacgggaaa actccgggcc ttccggcgca atagtaatac gctgaaccat aatcgcttcc
     7321 tcttatcaga tataagagga gtatacgcaa gattaggttc aaaagagtga tggttgctcc
     7381 ggttcgtctg atgacgctgg cttatttgcg cgtaatttgc gcattaatcg ctgccgacaa
     7441 aggcgcagca cctcttgttt ttcgccatcg ctcattttat tccagttaaa acgctcatcc
     7501 cgactacgaa aacagccgcg acaaaacccg cgttcgtcag actggcaaat tccccggcac
     7561 gggctctgga cgggaaagaa ctctaattgc tccgccacct cgccctcctc agataagatt
     7621 attaccatta ttgaagctgt taatgtccaa agtagcaact ttgcttgcac tagaccaact
     7681 ggtctactac actccaacgc atgaacaaac acaccgaaca tgatactcgc gaacatctct
     7741 tggcgacggg cgagcaactt tgcctgcaac gtggattcac cgggatgggg ctaagcgaat
     7801 tactaaaaac cgctgaagtg ccgaaagggt ccttctatca ctactttcgc tctaaagaag
     7861 cgtttggcgt tgccatgctt gagcgtcatt acgccgcata tcaccagcga ctggctgagt
     7921 tgctgcaatc cggcgaaggt aactaccgcg accgcatact ggcttattac cagcaaacac
     7981 tgaaccagtt ttgccaacat ggaaccatca gtggttgcct gacagtaaaa ctctctgccg
     8041 aagtgtgcga tctgtcagaa gatatgcgca gcgcgatgga taaaggtgct cgcggcgtga
     8101 tcgccctgct ctcgcaggct ctggaaaatg gccgtgagaa ccattgttta accttttgtg
     8161 gcgaaccgct acaacaggca caagtgcttt acgcactatg gcttggcgcg aatctgcagg
     8221 ccaaaatttc gcgcagtttc gagccactgg aaaacgcgct ggcccatgta aaaaacatta
     8281 ttgcgacgcc tgccgtttag caggcatttt ttatcaccag acgaccggga gcctttatgt
     8341 catctgaaaa actgtattcc ccactgaaag tgggcgcgat cacggcggca aaccgtattt
     8401 ttatggcacc gctgacgcgt ctgcgcagta ttgaaccggg tgacattcct accccgttga
     8461 tggcggaata ctatcgccaa cgtgccagtg ccggtttgat tattagtgaa gccacgcaaa
     8521 tttctgccca ggcaaaagga tatgcaggtg cgcctggcat ccatagtccg gagcaaattg
     8581 ccgcatggaa aaaaatcacc gctggcgttc atgctgaaaa tggtcatatg gccttgcagc
     8641 tgtggcacac cggacgcatt tctcacgcca gcctgcaacc tggcggtcag gcaccggtag
     8701 cgccttcagc acttagcgcg ggaacacgta cttctctgcg cgatgaaaat ggtcaggcga
     8761 tccgcgttga aacatccatg ccgcgtgcgc ttgaactgga agagattccg ggtatcgtca
     8821 atgatttccg tcaggccatt gctaacgcgc gtgaagccgc ttttgatctg gtagagctcc
     8881 actctgctca cggttatttg ctgcatcagt tcctttcccc ttcttcaaac catcgtaccg
     8941 atcagtacgg cggcagcgtg gaaaatcgcg cacgtttggt actggaagtg gtcgatgccg
     9001 ggattgaaga atggggtgcc gatcgcattg gcattcgcgt ttcgccaatc ggtactttcc
     9061 agaacacaga taacggcccg aatgaagaag ccgatgcact gtatctgatt gaacaactgg
     9121 gtaaacgcgg cattgcttat ctgcatatgt cagaaccaga ttgggcgggg ggtgaaccgt
     9181 atactgatgc gttccgcgaa aaagtacgcg cccgtttcca cggtccgatt atcggcgcag
     9241 gtgcatacac agtagaaaaa gctgaaacgc tgatcggcaa agggttaatt gatgcggtgg
     9301 catttggtcg tgactggatt gcgaacccgg atctggtcgc ccgcttgcag ctcaaagctg
     9361 agcttaaccc acagcgtgcc gaaagtttct acggtggcgg cgcggaaggc tataccgatt
     9421 acccgacgtt gtaatccaac attgcgagcg gcgtaaagcc gccgctatac taaaacaaca
     9481 ttttgaatct gttagccatt ttgaggataa aaagatgcgt cttcttcata ccatgctgcg
     9541 cgttggcgat ttgcaacgct ccatcgattt ttataccaaa gtgctgggca tgaaactgct
     9601 gcgtaccagc gaaaacccgg aatacaaata ctcactggcg tttgttggct acggcccgga
     9661 aaccgaagaa gcggtgattg aactgaccta caactggggc gtggataaat acgaactcgg
     9721 cactgcttat ggtcacatcg cgcttagcgt agataacgcc gctgaagcgt gcgaaaaaat
     9781 ccgtcaaaac gggggtaacg tgacccgtga agcgggtccg gtaaaaggcg gtactacggt
     9841 tatcgcgttt gtggaagatc cggacggtta caaaattgag ttaatcgaag agaaagacgc
     9901 cggtcgcggt ctaggcaact aatctcctgc cgggcgtgaa ctcatcgcgc ccgcatcttt
     9961 actgcatcga caagtaatat ttgtcataat gcgcgctgca atttatccgt attaagagaa
    10021 tcagatgtcc gataacgctc aacttaccgg tctgtgcgac cgttttcgtg gtttttatcc
    10081 tgttgtgatc gatgttgaaa cagccggatt taacgccaaa accgatgcgc tgcttgagat
    10141 tgccgccatc accctgaaaa tggatgaaca aggctggctg atgccggaca ccacattaca
    10201 tttccacgtc gaaccatttg tcggcgcaaa tttgcaacca gaagccctcg ccttcaacgg
    10261 cattgacccg aacgatcccg atcgcggcgc ggtcagcgaa tacgaggcgc tgcacgaaat
    10321 ttttaaagtt gtacgtaaag gtattaaagc gagcggctgt aaccgcgcca ttatggtggc
    10381 gcacaacgcc aattttgatc acagctttat gatggccgcc gcagaacgcg cctcgctgaa
    10441 acgtaacccg ttccaccctt tcgccacttt tgacactgct gcactggccg ggctggcact
    10501 cggacaaacc gtattgtcaa aggcttgcca gaccgctggc atggacttcg acagcaccca
    10561 ggcgcactcc gcgctgtacg acaccgaacg cactgctgtg ctgttttgtg aaatcgtcaa
    10621 ccgctggaaa cgtctgggag gctggccgct acctgccgcc gaagaggtgt aatcgagtcg
    10681 atgcctgatg acatgcaatg attcaggcat ctatagtgag gctattccac gcatcaagca
    10741 tgatattcac ggggaatagc gttaatggca gataatccag acccttcatc gctcctgccg
    10801 gacgtgtttt caccggcgac ccgcgactgg tttcttcgcg cctttaaaca gccgaccgct
    10861 gtccagtcgc aaacctggca tgtggcggcg cgaggcgaac atgcgctggt gattgcaccg
    10921 actggctccg ggaaaacgct ggcagcattt ctctacgccc tcgatcggct cttccgcgaa
    10981 ggcggcgaag atacccgcga ggcgcataag cgtaaaacct cacgcatcct ctatatttca
    11041 ccgataaaag ccctgggcac cgacgttcag cgcaacttgc agatcccgtt gaagggtatt
    11101 gccgatgaac ggcggcggcg cggcgaaacg gtaattattg atgaagtcca cgcggtagcg
    11161 ggcagtaaac gtggtgcgca tctggcgtta agtctggagc ggctcgatgc gctgctccac
    11221 acctcagcac agcgaattgg cctttctgcc actgtgcgct cagccagcga tgtggcagca
    11281 tttcttggtg gcgatcgccc ggttacggta gtcaacccgc ccacaatgcg ccatccgcag
    11341 atacgaattg tcgtaccggt cgccaatatg gatgatgtct catcggtcgc cagcggcacc
    11401 ggcgaagaca gccatgccgg ccgggaaggc tccatctggc catatattga aacgggtatc
    11461 cttgatgaag tgttgcgcca tcgctcgacc attgtcttta ctaattcacg cgggctggcg
    11521 gaaaaactga cggcacgatt aaatgagctt tacgccgcac gcttacagcg ttccccgtct
    11581 atcgccgttg atgcggccca tttcgagtcg acctccggcg caacctctaa ccgtgtacaa
    11641 agtagcgacg tttttattgc ccgctcacac cacggctccg tctctaaaga acaacgagca
    11701 atcaccgaac aggcgctgaa atcgggtgaa ttacgctgcg tggtcgcaac ctccagtctt
    11761 gaactgggga ttgatatggg cgcggtggat ctggtgattc aggtggcaac gccgctttct
    11821 gttgccagtg ggttacaacg cattggtcgc gccggacatc aggttggcgg tgtatctaaa
    11881 gggctgtttt tcccccgtac ccggcgtgat ttagtcgatt ccgcagtcat tgtagagtgt
    11941 atgttcgcag gcaggctgga aaacctgaca ccaccgcata atcctctcga cgtccttgcg
    12001 cagcaaaccg ttgccgccgc ggcgatggat gcattacagg tagacgaatg gtactcccgc
    12061 gtacgccgta ccgcaccgtg gaaagatctg ccaagacgtg tttttgacgc cacgctggat
    12121 atgctttccg ggcgctatcc ctctggcgat ttttctgctt ttcgccccaa actggtctgg
    12181 aacagggaga ccgggatatt gaccgcccga cctggcgctc aattgttggc ggttaccagc
    12241 ggcggcacca ttccggatcg tggcatgtat agcgtgttat tacccgaagg tgaagaaaag
    12301 gccggttcgc ggcgggtggg tgaactggat gaggagatgg tatatgagtc gcgggtgaac
    12361 gacattatca ctctcggcgc tacctcatgg cggatccagc aaatcacccg cgatcaggtg
    12421 attgtgactc ctgctccggg tcgttctgcc cagctcccct tctggcgtgg tgaaggtaac
    12481 ggacgtccgg ctgaattagg cgagatgatc ggcgattttc ttcatttact ggcggatggc
    12541 gcgttctttt ccgggactat tcccccgtgg ctggcagaag aaaatacgaa cgccaatatt
    12601 caggggttga ttgacgagca acgcaacgcg acgggcatcg ttccggggag tcgccatctg
    12661 gtcctcgaac ggtgccgtga tgaaattggc gactggcgta ttattttgca ctctccctat
    12721 ggaagacggg tgcatgaacc ctgggcgctg gcgattgccg ggcgaataca tgccctatgg
    12781 ggcgctgacg cgtctgtggt cgccagtgat gacagcattg ttgcgcgcat tcctgacact
    12841 gatggtaaat tgcccgacgc cgcgattttt ttgttttaac cagaaaagtt gctgcaaatt
    12901 gtccgcgagg cggtaggcag ctcggcactt ttcgccgccc gttttcgcga atgcgccgcg
    12961 cgggcattat taatgccggg ccgcacgccg ggccatcgca ccccgctttg gcaacaacgg
    13021 ctgcgcgcca gtcagttgct ggaaatcgct cagggatatc cggattttcc ggtcattctc
    13081 gaaaccctac gcgaatgtct gcaagatgtt tatgatcttc ccgcactgga acgtttgatg
    13141 cgtcgcctga acggtggcga aattcaaata tccgatgtaa cgaccactac gccctcgcct
    13201 ttcgccacaa gtttattgtt cggctatgtc gcggaattta tgtaccagag cgacgccccg
    13261 ctggcagagc gccgggcatc cgtactgtcg ctggacagcg agttactgcg caatctactc
    13321 ggacaggtcg atccggggga attactcgac ccgcaggtca ttcgccaggt ggaagaagag
    13381 ttgcaacgac tggctcctgg tagaagagcg aaaggtgaag aaggattgtt cgacctgctg
    13441 cgcgaactgg ggccaatgac cgttgaagac ctggcgcaac ggcatacagg cagcagtgaa
    13501 gaggttgcgt cgtatctgga aaatcttctt gcagtaaaac gaatcttccc agcgatgatt
    13561 agcggacagg agcgtcttgc ctgtatggat gatgccgcca ggctgcgtga tgccctcggc
    13621 gtacggctac cagagtcatt gccagagatt tatttacata gagtcagtta cccgcttcgc
    13681 gacctctttc tgcgctatct ccgggctcat gctctggtca cggctgaaca actggctcat
    13741 gagtttagtc tcggtattgc cattgtcgaa gagcagcttc agcaactgcg tgaacagggt
    13801 ctggtgatga atctgcaaca agacatctgg gtgagcgatg aagtatttcg tcgtctgcgt
    13861 ttgcgctcgc tgcaagccgc cagagaagcg acgcgtcccg ttgcagccac gacctatgcg
    13921 cgattgctgc tggaacgtca gggcgtatta cccgccaccg atggtagccc ggcgctcttt
    13981 gcctcaacat ctccaggcgt ttatgagggc gtagatggag tgatgcgggt gatcgaacag
    14041 cttgccggag tcggtttacc cgcctcactc tgggaaagcc agatcctgcc tgcccgcgta
    14101 cgcgactatt cgccagaaat gctcgatgaa ttactggcaa ccggagcggt tatctggtcg
    14161 ggacaaaaaa aagctgggtg aagatgacgg cctggtggca ctgcatctac agaaatatgc
    14221 cgcagaatcg ttcactcccg ccgaagcgga tcaggcgaat cgttcggcgc tgcagcaagc
    14281 gataatcgct gttctggctg acggaggagc ctggtttgca caacaaatca gccaacggat
    14341 acgcgataaa atcgtcgaat cggttgattc ctcggcactg caagaggcgt tatgggcgct
    14401 ggtctggcaa ggcgtcatta ccagcgatat ttgggcaccg ttacgcgccc tcacccgcag
    14461 cagttccaac gcacgcacct caactcgccg cagtcaccgg gctcgtcgtg ggcgtcctgt
    14521 ctatgcacaa cccgtctcgc cgcgggtatc ttacaacaca ccaaatctgg ctggacgctg
    14581 gtcgttattg caggtggagc cactaaacga taccgaaagg atgctggcgc tggcggaaaa
    14641 tatgctcgac cgctacggca tcatcagtcg tcaggcggtg atagccgaaa atatccctgg
    14701 cgggtttcca tcgatgcaaa cactttgtcg aagtatggaa gactccgggc gaattatgcg
    14761 aggtcgtttt gtagaaggtc tgggtggcgc gcaattcgct gaacgtctga ctattgaccg
    14821 attgcgcgat ctggcgacac aagccacgca aacacgccac tatacaccag tggcgctctc
    14881 cgccaacgat ccggctaatg tgtggggaaa tcttctgccc tgggctgcac atccggcaac
    14941 gctggtacca acgcgtcggg cgggtgcgct ggtggtcgtt tctggcggca aattgttact
    15001 ctatctggcg caaggcggca aaaaaatgct ggtctggcag gaaaaggagg aattactcgc
    15061 cccagagatt ttccaggcgc tgaccaccgc actgcgtcgc gaaccaaggc tgcgctttac
    15121 gctaacagaa gtgaatgatc ttccggtccg gcaaacgccg atgtttacgc tgctgcgtga
    15181 ggcgggattt tcaagttcgc cacaagggct ggattgggga tagttgacat cctccacgcc
    15241 ctgaaagacg tggttccctg ctacgttcaa gctgtcgcct gaatcatttc ggtgggttcc
    15301 tgcttcaacg agcggcctga ctgcaccatc tctccacagg caagaacggc gtgccccgcc
    15361 gctaaaatgt tacgagcgcc atttacatcg gcgttcgctg tatatccaca tacctggcat
    15421 ctgaatttac tttgtgacag gcggttttct ttcgctgtat gaccacagca cgcgcaacgc
    15481 tggcttgtgt acgctggcgg aacagcaagc acctgaccgc cactccagag ctgcttgtac
    15541 tcaagctggc ggcgcatttc ataccagccc tgatccagta tcgaacggtt taaacctgat
    15601 tttgcccgga cattgcgccc cggctggctg accgtacccg ctgctgactt tgacatgttg
    15661 ctgaccttca aatcctcaat gactatcatt gcgtggtttt tgctgacggt cgttgtgact
    15721 ttgtgaaggt agtccctgcg gatatttgcg atacaggaat gcagtcgctg tattttgcgt
    15781 ttctgcttct gccagttgtt gctgaatttg accttccggc ttaactgtcg ctgaagtctc
    15841 gccagcgtct tctggttttt ttgaaaactg tttacaggct caaagactgt gccatctgac
    15901 agcgtggcga gtttagccac gccagcatcc agcccgatca ttgatgctga agggtgaacc
    15961 ggagttgata cttcactttc tgtctgaata ctgatgtacc acgtaccgca ggactggctg
    16021 acagtgacat ttttcacaac tcccgtgacc tgacggctat tccggtagcg catccagcca
    16081 agtttcggca gaaaaatacg gctgttttcc tgatcgagct taacgtcctg cgggtagcgg
    16141 aatgcagcat tctgtccccg ctttttgaat cggggaaaag cagcccgctt ctggaagaag
    16201 tttttgtaag cccgctcaag gtctttcagt gactgttgca atggctgtga gggcgaatct
    16261 ttaagccatt gcgtttcagt ggcgtttttc cactcaacca gccaggaagc cattttcccg
    16321 taagggatgt atttattacc ggcctcatga ttctcattct gaagcgccag agcacgattg
    16381 aaaacgaaac gacatgcgcc tgcgaagcgc ctcatttcac gctcctgttg accaccgggt
    16441 cttaactgga atttaaatgc ctgtaatcgt ttcacaggtc aaatgatact ttggtctgtg
    16501 aaaaaagaaa ccgatatccg gcgtggaagg cattgtgttt tcctgatgca tgttcacctg
    16561 gtctttgtca ccaaataccg acgccagatt tttgaccatg acacgacaga aaaactacgc
    16621 acttactttt caaatgtatg tgctgatttt gcagctgaac tggttgaaat ggatggcgaa
    16681 ccagatcacg tccatttgtt aattaactat cctcccaaac tggcgatatc cagtctggta
    16741 aacagcctca aaggcatatc gaataggtta ctgcgacgag atcgaccaga tattgcagtc
    16801 aggtattact acaaaggcgt tttgtggagt cctggctatt ttgccagtag ctgcgaaggt
    16861 gcgccaatat ccgtcatccg ccagtacatt gaacaacagc aaacacctgg tcaggtggaa
    16921 aaccgcgcct tatatccccg ccctgaagga cgaggtttta cggcgcaccg gataaaggac
    16981 tgacggatgc ccgttcgcat ccgtcagtat tgcaggacgg attattccgc gtccggctct
    17041 tcagacttgt atttagcggc agtttctttg atcagctgct gcagttcgcc acgctgatac
    17101 atttcgatca cgatatcaca accgccgacc agctcgccgt caacccacag ttgcgggaag
    17161 gtcggccagt tagcatattt cggcagttcc gcacgaatgt ccggattctg cagaatatca
    17221 acataggcaa aacgttcgcc acatgcggca agcgcctgga ctgcctgggc agagaaaccg
    17281 cagctcggca gtttcggtga acctttcatg tacagcagga tcgggttttc agcaatctgg
    17341 cgttggattt tttcgatagt ggtgctcatt gtcttgcttc ctcaaacttc tttacggcag
    17401 taatctgaca ttgtagcggg tcagtgcggc atcggaaaat aacattttca tcacgctttt
    17461 gctattttat ccctttgctc tatttgttgc atttcaaata ttcgtttctt ctctgatgcg
    17521 cggcaaactg gcgtatttat aaccattttt agctttcact gctgcatttt tttgcgctcg
    17581 ccaacgaaac gtatttttta acaataaaag ctattaactt tctcttcttc tatgcattag
    17641 aatcatcaag ttttgtaaat cagacgcagg catgatagac ctgccttaac agagggacgc
    17701 tcagtggcgc ggataaaccg tatttcgatc acgctctgtg ctttactttt taccaccctg
    17761 cctttaacgc ctatggccca tgcttcaaag caagccaggg agagttctgc taccactcat
    17821 atcaccaaaa aagcagataa aaagaaaagc acggcaacca ccaaaaagac ccagaaagcc
    17881 agccagaaaa cagcgaaaaa agccgccagt aaaagtatga ccaaaagcaa aaccgcttct
    17941 tctgttaaaa aatcttccat taccgcttct aaaaacgcca aaactcgcag caaacacgcc
    18001 gtcaataaaa cggcctcagc cagctttacc gaaaagtgta ccaaacgtaa gggctataaa
    18061 tcgcattgtg tgaaagtcaa aaatgccgcg tcaggaaccc ttgccgacgc gcacaaagcg
    18121 aaggtgcaaa aagccacgaa agtggcaatg aataaactga tgcagcaaat tggtaagcca
    18181 tatcgttggg gcggcagctc accgcgtacc ggttttgatt gcagcggcct ggtttattac
    18241 gcttataaag atttggtgaa aattcgtatt ccgcgtacgg cgaatgaaat gtatcacctg
    18301 cgtgatgcag gacctatcga acgtagtgag ctgaaaaacg gcgacctggt ctttttccgt
    18361 actcagggac gcggtacagc cgatcatgtc ggcgtgtatg tcggcaacgg caaatttatt
    18421 cagtcaccgc gcacaggtca ggaaattcaa atcacttctc tcagtgaaga ctactggcag
    18481 cgccactatg ttggcgctcg tcgggtaatg accccaaaaa cacttcgcta aaactttacc
    18541 ctgttgttac ggcaacaggg taagttcatc ttttgtctca ccttttaatt tgctacccta
    18601 tccatacgca caataaggct attgtacgta tgcaaattaa taataaagga gagtagcaat
    18661 gtcattcgaa ttacctgcac taccatatgc taaagatgct ctggcaccgc acatttctgc
    18721 ggaaactatc gagtatcact acggcaagca tcatcagact tatgtcacta acctgaacaa
    18781 cctgattaaa ggtaccgcgt ttgaaggtaa atcactggaa gagattattc gcagctctga
    18841 aggtggcgta ttcaacaacg cagctcaggt ctggaaccat actttctact ggaactgcct
    18901 ggcaccgaac gccggtggcg aaccgactgg aaaagtcgct gaagctatcg ccgcatcttt
    18961 tggcagcttt gccgatttca aagcgcagtt tactgatgca gcgatcaaaa actttggttc
    19021 tggctggacc tggctggtga aaaacagcga tggcaaactg gctatcgttt caacctctaa
    19081 cgcgggtact ccgctgacca ccgatgcgac tccgctgctg accgttgatg tctgggaaca
    19141 cgcttattac atcgactatc gcaatgcacg tcctggctat ctggagcact tctgggcgct
    19201 ggtgaactgg gaattcgtag cgaaaaatct cgctgcataa taactgatgg caaatgcagc
    19261 attgcctgat gcgcttcgct taccatgcca acgcggatga ggcgttaaca gcgcattcgg
    19321 caagataaat cgcacgttgt cagcaactgt aacgcagaag gttatccttc tgcgtttttg
    19381 tttaattagc tgttagcaac gcaaactgtt tcaggttgtt ttctggctga cataaacacc
    19441 agcaataatg ccagtcccgc gacaatcgcc cccgtcaccg gcacaaagct gtatcccagc
    19501 ccagcggaaa ttaccgcacc accagcagct gctcccagcg catttccaag attaaaggca
    19561 ccaatattga ctgatgaaga cagacctggc gcttcactgg cgacatgcat cacgcgcatc
    19621 tgtaacggag gtacaaccgc aaaggttgct gcgccccaca ccaccatgct aatagctgcg
    19681 ccgaactcat tgcgggccag gaacgggatt gccagcataa tcaccatcag cagcaacaaa
    19741 aagcctttca acgtgccgtt aactgaacga tctgccagtt tgccgccgag atagttaccg
    19801 atagagaatc cgacaccaat cagcaccagc attgccgtga cgaacaccgg tgttgcgtgg
    19861 gtaatacttt gcagtaccgg agagatatag gtgtagaggg taaacattgc accagctccc
    19921 agtaccgtcg tcagcaatgc agacagcacc tgcggacgca ttaataccgc cagctctttt
    19981 ttcacttcag gtcgtgcccc tgtaccacct ttaggtaatg agaagaacag acttaccatt
    20041 gaaatcactc ccagccccgc cgttgccaga aatgacatcc gccagccgat ggtttcaccc
    20101 aaccaggtcg ccgccggcac gccaccgata tttgccaggg ttaaccccat aaacatagtg
    20161 gcaactgcgc tggcctgttt atgttttggc accacgcttg cggccacgac tgaacccaaa
    20221 ccaaaaaatg ctccgtgatt caggctggtc aaaatacgtg aaagcatcag ggtcatataa
    20281 tccggcgcga tggcggaaag aacgttgcct agcgtgaaaa ttgccatcag gaaaatcaac
    20341 gcactgcggc gggcacgatg agaaagtaga agcgtcatca gcggcgcgcc caccattacg
    20401 ccaactgcat aggcactgat taacattccg gcagcgggaa tcgagacatc cacaccgcgc
    20461 gcaatgacgg gcaacaagcc cattggcgag aactccgttg tcccgatacc aaacgcgcca
    20521 atcgccagcg ccagcaacgg atagttaatt ttcatgcctt atctccacct cttcgcgtca
    20581 ttacgcgata ttcattaaag tggcgaaagc atgacagcaa tcacaaaaaa atgaaaataa
    20641 caaaaagaga aaacactttt gccattttgc taacaaacag gaaggagatg cgagggagaa
    20701 cgcgctccct cgagaggaaa tcagtgcagc gcggcagtca aacccacggc tacgatcaaa
    20761 ccgaggacga taaccgttgt taccagtgaa aatttaaggt cggtgctcat caagttttct
    20821 ccttttttat taccacacaa aaagtgatat tacgcatttt tacacactgt gatgaaaaaa
    20881 tctcccgtca tttataatga taagtgtttt taccacttcc ccttttcgtc aagatcggcc
    20941 aaaattccac gcttacacta tttgcgtact ggccattgac cccttcctga cgctccgtgt
    21001 cgtttttccg gcgtaccgca acacttttgt tgtgcgtaag gtgtgtaaag gcaaacgttt
    21061 accttgcgat tttgcaggag ctgaagttag ggtctggagt gaaatggaat ggcaacaata
    21121 aaagatgtag cgaaacgagc aaacgtttcc actacaactg tgtcacacgt gatcaacaaa
    21181 acacgtttcg tcgctgaaga aacgcgcaac gccgtgtggg cagcgattaa agaattacac
    21241 tactccccta gcgcggtggc gcgtagcctg aaggttaacc acaccaagtc tatcggtttg
    21301 ctggcgacca gcagcgaagc ggcctatttt gccgagatca ttgaagcagt tgaaaaaaat
    21361 tgcttccaga aaggttacac cctgattctg ggtaatgcgt ggaacaatct tgagaaacag
    21421 cgggcttatc tgtcgatgat ggcgcaaaaa cgcgtcgatg gtctgctggt gatgtgttct
    21481 gagtacccag agccgttgct ggcgatgctg gaagagtatc gccatatccc aatggtggtg
    21541 atggactggg gtgaagcaaa agctgacttc accgatgcgg tcattgataa cgcgttcgaa
    21601 ggcggctaca tggccgggcg ttatctgatt gaacgcggtc accgcgaaat cggcgtcatc
    21661 cccggcccgc tggaacgtaa caccggcgca ggccgccttg ccggttttat gaaggcgatg
    21721 gaagaagcga tgatcaaggt gccggaaagc tggattgtgc agggtgactt tgaacctgaa
    21781 tccggttatc gcgccatgca gcaaatcctg tcgcagccgc atcgccctac tgccgtcttc
    21841 tgtggtggcg atatcatggc aatgggcgca ctttgtgctg ctgatgaaat gggcctgcgc
    21901 gtcccgcagg atgtttcgct gatcggttat gataacgtgc gcaacgcgcg ctattttacg
    21961 ccggcgctga ccacgatcca tcagccaaaa gattcgctgg gtgaaacagc gttcaacatg
    22021 ctgttggatc gtatcgtcaa caaacgtgaa gaaccgcagt ccattgaagt gcatccgcgt
    22081 ttgattgaac gccgctccgt ggctgacggc ccgttccgcg actatcgtcg ttaatcaccc
    22141 gttgcgggag tctcttccgg ctcccgcagc cactccttat tcagcgtctc actatcgctg
    22201 agatactcaa gcagccaggt taacgcaggc gacatatcat tttgctgcca tgtcagacaa
    22261 catgccgaat ccgggaaggg gttttccagt tcaagtgcta cccacttccc ctcattaagc
    22321 cacggtttgg cgaaatgtgt tggcaccatc cctatgcata atcctgccga gatacaggtt
    22381 gccgatgatt cccagtcagg tacgacaagt cttttttggt tatccagcaa ccaggtaata
    22441 cgtttaggta gcgttcgcga ggtgtcttcg cgcaccaacg acggccagtt gcgcaacgta
    22501 tcatcgctga acgggccatc catcaacgcc agcgggtggt gactggcaac aacgcaactc
    22561 cagcttagca tccccatatc ccggaaggca taacgaccgc ctaccggaat cgcgcgtgtt
    22621 gcgccaattg ccagttccac gcgcccgtcg gaaagcgcat cccagacacc gttgaacact
    22681 tcctgaaaga caagaagttc gacatcatca aaatggcgat aaaaatcaac gatcatctgc
    22741 cgtgtacgtt ctggcctgac aatattatcc actgcgatag ctaactgacc gcgccagccg
    22801 ttcgctatct gctgacattg ctggcgggtg atctgcattt ttttgacaac agagcgccct
    22861 tctttgagaa accacgctcc agcagcggtc agctccacat cacggtgccg tcgttcaaag
    22921 agcggtaccg ccagccactc ttccagctga cgcacggtat agctgaccgc agaaggaaca
    22981 cgatgcagct cctgtgccgc agcgctaaaa ctaccattac gcgctaccgc atcaacaact
    23041 tcgagtgaat attctgacca catagtctgc ctgcaaaatt tttgaaacca gtcatcaaat
    23101 attaccgttt cacaacacta atttcactcc ctacactttg cggcggtgtt taattgagag
    23161 atttagagaa tatacatgca acctgggaaa agatttttag tctggctggc gggtttgagc
    23221 gtactcggtt ttctggcaac cgatatgtat ctgcctgctt tcgccgccat acaggccgac
    23281 ctgcaaacgc ctgcgtctgc tgtcagtgcc agccttagtc tgttccttgc cggttttgcc
    23341 gcagcccagc ttctgtgggg gccactctcc gaccgttatg gtcgtaaacc ggtgttatta
    23401 atcggcctga caatttttgc gttaggtagt ctggggatgc tgtgggtaga aaacgccgcc
    23461 acgctgctgg tattgcgttt tgtacaggct gtgggggtct gcgccgcggc ggttatctgg
    23521 caagcattag tgacagatta ttatccttca cagaaagtca accgtatttt tgcgaccatc
    23581 atgccgctgg tgggtctgtc tccggcactg gctcccctgt taggaagctg gctgctggtc
    23641 catttttcct ggcaggcgat tttcgccacc ctgtttgcca ttaccgtggt gctgattctg
    23701 cctattttct ggctcaaacc cacgacgaag gcccgtaaca atagtcagga tggtctgacc
    23761 tttaccgacc tgctacgttc taaaacctat cgcggcaacg tgctgatata cgcagcctgt
    23821 tcagccagtt tttttgcatg gctgaccggt tcaccgttca tccttagtga aatgggctac
    23881 agcccggcag ttattggttt aagttatgtc ccgcaaacta tcgcgtttct gattggtggt
    23941 tatggctgtc gcgccgcact gcagaaatgg caaggcaagc agttattacc gtggttgctg
    24001 gtgctgtttg ctgtcagcgt cattgcgacc tgggctgcgg gcttcattag ccatgtgtcg
    24061 ctggtcgaaa tcctgatccc attctgtgtg atggcgattg ccaatggcgc gatctaccct
    24121 attgttgtcg cccaggcgct gcgtcccttc ccacatgcaa ccggtcgcgc cgcagcgttg
    24181 cagaacactc tgcaactggg tctgtgcttc ctcgcaagtc tggtagtttc ctggcttatt
    24241 agtatcagca cgccattgct caccaccacc agcgtgatgt tatcaacagt agtgctggtc
    24301 gcgctgggtt acatgatgca acgttgtgaa gaagttgact gcccgaatca tggcaatgcc
    24361 gaagtcgctc atagcgaatc acactgatct atatcgatat acttatactt aggctgctaa
    24421 caaaattttg ttgtatgatt gaaattagcg gcctatacta atttcgagtt gttaaagcta
    24481 cgataaatat tatgttttta cggggacagg atcgttcccg actcactatg gatggtcatt
    24541 tcggcaaggg ttcctccttt ccctctgttc tacgtcggat tatagactcg cggttttttc
    24601 tgcgagattt ctcacaaagc ccaaaaagcg tctacgctgt tttaaggttc tgatcaccga
    24661 ccagtgatgg agaaactatg agttcatcgt gtatagaaga agtcagtgta ccggatgaca
    24721 actggtaccg tatcgccaac gaattactta gccgtgccgg tatagccatt aacggttctg
    24781 ccccggcgga tattcgtgtg aaaaaccccg atttttttaa acgcgttctg caggaaggct
    24841 ctttggggtt aggcgaaagt tatatggatg gctggtggga atgtgaccga ctggatatgt
    24901 tttttagcaa agtcttacgc gcaggtctcg agaaccaact cccccatcat ttcaaagaca
    24961 cgctgcgtat tgccggcgct cgtctcttca atctgcagag taaaaaacgt gcctggatag
    25021 tcggcaagga gcattacgat ttgggtaatg acttgttcag ccgcatgctt gatcccttca
    25081 tgcaatattc ctgcgcttac tggaaagatg ccgataatct ggaatctgcc cagcaggcga
    25141 agctcaaaat gatttgtgaa aaattgcagt taaaaccagg gatgcgcgta ctggatattg
    25201 gctgcggctg gggcggacta gcgcactaca tggcatctaa ctatgacgta agcgtggtcg
    25261 gcgtcaccat ttctgccgaa cagcaaaaaa tggctcagga acgctgtaaa ggcctggatg
    25321 tcaccatttt gctgcaagat tatcgtgacc tgaacgacca gtttgatcgt attgtttctg
    25381 tggggatgtt cgagcacgtc ggaccgaaaa attacgatac ctattttgcg gtggtggatc
    25441 gtaatttgaa accggaaggt atattcctgc tccatactat cggttcgaaa aaaaccgatc
    25501 tgaatgttga tccctggatt aataaatata tttttccgaa cggttgcctg ccctctgtac
    25561 gccagattgc tcaatccagc gaaccccact ttgtgatgga agactggcat aacttcggtg
    25621 ctgattacga tactacgttg atggcgtggt atgaacgatt cctcgccgca tggccagaaa
    25681 ttgcggataa ctacagtgaa cgctttaaac ggatgtttac ctattatctg aatgcctgcg
    25741 caggtgcttt ccgcgcccgt gatattcagc tctggcaggt ggtgttctca cgcggtgttg
    25801 aaaacggcct tcgagtcgct cgctaaaggc tattctatcg ccccctcttc gggggcgagt
    25861 tcagatcagg cttctgtacc tggttgattc atggcatttt ctcgtgccgc cagcacacgt
    25921 tctaccgtat ctaccactgc ctgagtttgt ggatcgattt caatgttgac gcgtgcgcca
    25981 agttttttct tcccaagagt cgtgcgttcc agtgtttccg gaattaaatg gacgcaaaaa
    26041 cgcgttggcg tgacttcgcc gacggtcagg ctaataccgt cgatgccaat aaatcctttg
    26101 tacaggatat atttcatcaa ctgactatcc tggactttaa accagatctg gcgattattt
    26161 tccgaggtta atattttcgc cacttcagcg gtggtcataa tatgacctga cattaagtgt
    26221 ccgccaattt catcactgaa tttcgccgca cgctcaacgt ttacccaatc ccccactttt
    26281 aaatcgccaa gattggtaat gcgtaacgtt tctttcatca ggtcaaaact gacatggttg
    26341 ccgttaattt ccgtcacggt caggcagcaa ccgttatgcg ccacggaagc accggtttcc
    26401 aggccgtcca gcatgtggtc gggtaactcc accacatgcg tacgaaaatt tggtttctcg
    26461 tcaatcgaca ccagttttgc ggtgccctgt acaatccccg taaacatact tacaactcct
    26521 gaaatcagtt aagacattct gttcagcaca atagcaggtg gaaaacgccc ttaccagtga
    26581 aggggtaaga atggctattt tttcactgga gaattaataa atcctcgcta caatagactg
    26641 aatttcccct gcttcttctt tttgctgccc attcaggcgg ctttttagtc tctcatataa
    26701 ctacaaataa aaggtgttca cgtgcagaag tatatcagtg aagcgcgtct gttattagca
    26761 ttagcaatcc cggtgattct cgcgcaaatc gcccaaactg cgatgggttt tgtcgatacc
    26821 gtgatggcgg gcggctatag tgccaccgac atggcggcgg tcgctatcgg tacttctatc
    26881 tggcttccgg cgatcctctt tggtcacgga ctgctgctgg cattaacgcc ggttatcgcg
    26941 caattaaatg gttccggtcg acgtgagcgc attgcgcatc aggtgcgaca aggtttctgg
    27001 ctggcaggtt ttgtttccgt tctcattatg ctggtgctgt ggaatgcagg ttacattatc
    27061 cgctacatgg aaaacatcga tccggctctg gcggacaaag ccgtgggtta tctgcgtgcg
    27121 ttgttgtggg gcgcgccggg atatctgttc ttccaggttg cccgtaacca atgtgaaggt
    27181 ctggcaaaaa ccaagccggg tatggtaatg ggctttatcg gcctgctggt gaacatcccg
    27241 gtgaactata tctttattta tggtcatttc ggtatgcctg agctcggtgg cgttggttgt
    27301 ggcgtggcta ctgcggcggt gtattgggtc atgttccttg ccatggtttc ttacattaaa
    27361 cgcgcccgct ccatgcgcga tattcgtaac gaaaaaggca ccgctaagcc cgagcctgcg
    27421 gttatgaaac gactgattca actcggtttg ccgattgcgc tggcactgtt ctttgaagtg
    27481 acactgtttg ccgtcgtggc tctgttagtg tctccgctcg gtattgttga tgtcgcagga
    27541 caccagattg ccctgaactt tagttcactg atgtttgtgc ttccaatgtc gctggcggca
    27601 gcggtaacta tccgcgtagg ttatcgtctg ggtcagggct caacgctgga tgcgcaaacc
    27661 gctgcgcgga ccgggcttat ggtgggtgtc tgtatggcaa ccctgacggc cattttcacg
    27721 gtttcactgc gggagcaaat cgccctgttg tacaacgaca atcccgaggt tgtaacgctg
    27781 gctgcgcatt tgatgttgct ggcggcggta tatcagattt ctgactcaat ccaggtgatt
    27841 ggcagtggga ttttgcgtgg ttataaagat acgcgttcca ttttctatat cacctttaca
    27901 gcttactggg tgctgggctt gccaagcggc tatattctgg cactgaccga tctggtcgtt
    27961 gaacctatgg ggccagcagg cttctggata ggctttatta tcggcctgac atcggcagcc
    28021 attatgatga tgctgcgtat gcggttcctg caacgtatgc cgtcagccat cattctgcaa
    28081 cgagcgtccc gctaataaag acaaggcgca accttcacgg gttgcgcctg tgtttttacg
    28141 caggctggag cgttgcgcca atcccgtctt cgtctggctg taatttcaga gcgttacaca
    28201 gagtttgccg actggcatct gttatctcaa cgcgtatatc cccgttaact tcccctttca
    28261 cattgatgaa gtcaggtgaa atttctttcg cactggtttg caggcaaatc gctccctctc
    28321 cgcgcaggcg tttcactttc agttgcacat gcgttgccgc accagtgatc agccacagtt
    28381 ttccgccatt cagattgatg ttaccaacaa tgaaagtgac gctgtcacct tttaccgtac
    28441 tgccgtcgag aatcaacaca ccgccctgct gtacagtaat cgttgattgt tgcagctctg
    28501 cgccataacc aataatcatt gaagcgccgt ccagtaatgc aacactcccg gtcagttcaa
    28561 ccggtgcgcg atcttcctct acgacaaaat tttcgtccgc gctggtgggt gacaacagga
    28621 tcactgttcc accttgtatt accgcatttt ccagcgtacc ggcatgcact tcagcccgcc
    28681 cccccacatt caccgacagg ttttgcgtct taccggaact gtaaagctga aggccatccg
    28741 tccccagacg ataaatggat ccattctcaa caatagtgtc agagtcttca ccaccgagat
    28801 tattcatggt gccaccagca agcaacgtat cactggcttt cccggccagc ataaaaacgc
    28861 cgccctgatt aatctcacaa ttgctggctt ctccacttgg atacaccaac aattcaccac
    28921 cttcatctac cacgaccgct gaggttgtcc cattaaccaa caggccgccc tcttgatcga
    28981 gaatgatttt ttccgcgcga tgtccttcca gtacacgcag gttaccgcca ttttccagca
    29041 acaaaccgca ggcataaccc tgatcaacgc tgaattcacc ttcgggatgg cggccattaa
    29101 ccgtagcgag cgtagagaga ctaattgcgc ctccctcgga ctgaacaata tctgtggcaa
    29161 tggcaccgtc gctgaccgat agcgtgccat tgttaccgta ctgtgtgcat ttggcgatcc
    29221 caccggttgc cactttcaac cagccagccc ccgtgaccac ggtattttcg gctcttccgc
    29281 cagcttcaac aatcaactcg ccttcagtaa gttgcgtcgc gccaaggacc tcgccggtct
    29341 taacaatttg cacattcccg tcgctcatca agtttttcgc atcagatccc atgatttatt
    29401 cctttgctgc atctgtgtgc ctttattgct acctaagtgt aaaggctacg gaggatttat
    29461 ccacgacaga tttgagatgg tggcaaacaa ctctgtttaa actctgatac acgaattatt
    29521 gggttgtatc agatgtaaat gcgatcctga ataaaaatcg cccttgcaaa tcaacaaaat
    29581 atagccaatt tgcttaaaag ctctgcaaac ggtcgaaata gcgcagaaaa ttacgttttg
    29641 cctcttgcca cctttccact ctgccgctaa tattcgtccc cgttgtcacc tacaacgttg
    29701 cgttcatagc tcagttggtt agagcaccac cttgacatgg tgggggtcgt tggttcgagt
    29761 ccaattgaac gcaccatcct gcgtccgtag ctcagttggt tagagcacca ccttgacatg
    29821 gtgggggtcg gtggttcgag tccactcgga cgcaccagat tttcttaatc tggtcttttc
    29881 ctttttccct ctgtttcttc tctgtatcca atacgttaaa agatttacac tgtcttcgta
    29941 tgcgttatca gaaggagaat cgctatggca actttgttac aacttcattt tgcttttaat
    30001 ggcccatttg gcgacgcaat ggctgagcag cttaaaccac ttgctgagtc gattaatcag
    30061 gaacctggtt ttctgtggaa ggtatggaca gaaagtgaaa agaaccacga agccggtggg
    30121 atctaccttt tcactgatga aaaaagcgcc cttgcctatc tggaaaaaca tactgcccga
    30181 ctgaaaaatc tcggcgttga ggaagttgtc gccaaagttt tcgatgtcaa tgagccactt
    30241 agtcaaatca atcaggcaaa actcgcctga cagaatttaa tcaagggcgg ttagcgccct
    30301 tttcatccct gtctgaaatt tctcaaattc taaaaatctc aaccaaactt atctgataac
    30361 actaaattcg aaagaatgcg tacaggtaag taacaatgaa aaaaattgct attgtgggtg
    30421 ccgggcctac ggggatctac accttattct cgcttctaca gcaacaaact ccactttcta
    30481 tttctatctt cgagcaggct gacgaggccg gtgtcgggat gccatacagt gatgaggaaa
    30541 actcaaaaat gatgctggca aatattgcca gtattgaaat accgccgatt aattgtacgt
    30601 atctcgaatg gctacaaaag caagaagcca gccatctcca gcgttatggc gttaaaaaag
    30661 aaaccttgca cgatcgtcag tttttaccgc gaattctgct gggcgaatat ttccgcgatc
    30721 aatttttacg attagtagac caggcacgac agcaaaaatt tgcagtggct gtttatgaat
    30781 catgccaggt taccgatctg caaattacaa atgctggcgt catgctcgct acaaatcagg
    30841 atttacccag cgagacgttt gatttagcgg tgattgccac gggtcacgtc tggcctgatg
    30901 aagaagaagc aaccagaacg tattttccca gcccgtggtc aggcctgatg gaagcaaagg
    30961 tcgatgcgtg taacgtgggt attatgggaa catccttgag cggactggat gcggcaatgg
    31021 cagtggctat tcagcatggt tcgttcattg aagatgataa acaacacgtc gtttttcacc
    31081 gcgataacgc aagtgaaaag ctaaatatta cgttgatgtc gcgcacgggt attttacccg
    31141 aagccgattt ctattgccct attccctacg agcccttaca catcgtcacc gatcaggcat
    31201 taaatgctga gattcaaaaa ggtgaagagg gccttttgga tcgggtattt agattgatag
    31261 tagaggaaat caagtttgct gatccagact ggagccaacg catagcctta gagagcctga
    31321 atgtcgattc ctttgctcaa gcctggtttg ccgagcgcaa acaacgcgac ccatttgact
    31381 gggcagaaaa aaatctccag gaagtcgaac gcaataaacg agaaaaacat actgttccct
    31441 ggcgttatgt cattctgcgc ctgcatgaag ccgtacagga aattgttcct catctgaatg
    31501 aacacgacca taaacggttc agtaaaggcc ttgccagggt tttcatcgat aattatgcgg
    31561 caatcccttc agagtctatt cgtcgactac tggccttacg tgaagcgggg atctgaaatc
    31621 cctgatgttg gcgaagatta tacgttacag caacccgaag atattcgtgg acgcgtagcg
    31681 ttcggcgcgt tgccctggtt gatgcacgac cagcctttcg ttcagggact tacggcatgt
    31741 gcagaaattg gtgaggcgat ggctcgggcg gtcgtaaagc ctgcatcccg tgctcgtcgg
    31801 cgtctttcgt ttgattaaag gtaaagctat cagcactcaa caaactgatg ctgttcatta
    31861 agacgatagc ggactccggt tcgaatattg ttttcacctt caatggcgac ggcgaagcga
    31921 actctttcgc cgtcatgata tgccagtgcg gcgcttcccc ctagcccgag aataattgag
    31981 tcgaccacgc ccgtgctcac taccgttgaa ttttcaccgc tggcaatgat gtgtacattg
    32041 tcgccgctgt tggcaattct ggcgtttgcg ccaaagctgg cgatttgcac caggtcacca
    32101 ttactggcga catgacaccg ttcacccaat gtacaaacgc gtactcgcat tccggtattc
    32161 gcaatccgac tactatctcc agcgctgctt atccgcgaag aattaccggt tacagcaacc
    32221 cgtgcgcgtt caccggagct gccaatgtga ctgttaaagc cgacacttcc aatccgcacg
    32281 ctataaccca tactggcaat ttgcgcggca tacccagcgg ttgcgattcg ggcgttatcc
    32341 tcacaacagc cgatgcgagc atgatcgtcg ctgcgggcaa actgactgca aaatggtatc
    32401 tctccggggt ctgtggccag tttttgcata gaggaaactt cctgatgcgc gaagttttca
    32461 tccgccagcc attttttcca cgcatactca accagactct ccgcccagtc cgtatatcct
    32521 tcctcaatca gtgcatgacg aatatcggca taactaccgc cgcgtgggaa atggcgcagg
    32581 aaccagcgat acatcaccgc tccgatacgc cattctcgca ggtctgctcg tgtaatgatc
    32641 attcgttagt gccggtgata gccatcgacc atgcttttaa aggtttcgtg tggcgtacgc
    32701 cccgtggtgc aaagataaag atgaccgaag ataaaaaaga ggcttataaa tgccagagca
    32761 aaatgcgcct gcaataacca gtatcttacg ccaggaaaca catctcccac ggcttgcgga
    32821 tagagacaca acagccccgt caatagtaac aacggcagca atccatacat gacaccaaca
    32881 taggcgacct gctgtaaggg attaaattta gactgtgttg ttgccgggaa aggatgttct
    32941 tccccctgca taatgccaaa caaataaaat cgcgtttgtt ttgccgctcg ttccagccac
    33001 ccctgacgac gaatgcgata gtggtgacca ttatccccaa cggcattgat cagcacaaag
    33061 ccgagccagc atgccagtaa caaaaatccg caaacttcat gcaccgcaac cagactttta
    33121 accgcagttg cgcccaccat cgcaaagtga tttatcagcc cacttgccag caacaataca
    33181 aagagtaacg cattcgacca gtgccataat ctgaccgctt tgctgtaaag ataaaccttc
    33241 tcaccatgat cggtggctga tttcttcacg ccacgagcac gaagcaaggc gtgcagcccc
    33301 agcaccaacc acatcccaac cagcaatact ccggcaatga tcaaccacac cggccaaaat
    33361 tcgggagtga actgtggcac atagttcgct aactggctct gaaactgttc agcatgttgc
    33421 gacgggttca tacattttcc tttttaatca aatgttgacc gaaccggcga tacagatgcg
    33481 gttttccggc accaggtagc tgatattgat agtatttatt atcctgcaac cacgcctgaa
    33541 tttctgggct atcttcgcga ccaaagatca atgcgtgttc cggacaggcg ctgacgcaaa
    33601 tgggcggaaa gccttttgcc agtcgggact cagcgcagaa atcacattta tctgccactt
    33661 tagtgacagg gttaaggtag cgaaccaggt acggacaagc gccaatgcaa taactgcagc
    33721 cgatgcactg cgatttttct acccgcacga tcccctgctc atcacgccac gatgcccccg
    33781 tcgggcatac gtcgatgcac ggagcatctt cgcagtgctg acatgactgg cgaaaaaagt
    33841 gatactgcgt ttcgttgtcg ttatcggtga ctggaatgtg tgctatcgat aaacggcttc
    33901 cctgagcggg gacgtggtta gttttacggc atgcgcgggc gcaaatatta cagccattgc
    33961 accgtgactc gtcatgaatc atggcatacc gaacttcctt ttcttgcctc gtgttcgcta
    34021 acagagaaga cgcagaacca gtaaaaaaga ttactgttcc catccccaga acaaatttgc
    34081 gtcgagtgaa ggacatctga ttatccccta atgcagtggc tgcaactgtc caccctatcc
    34141 atgacatctc cctgaaattt caggggttct gaaactgctc tacctcgaac cattcgcata
    34201 agccataagc cttgccatag gtcataaatc gctcgctaaa caagtacaac accgccgtcg
    34261 atgactccac ctgccgtata tcggcatatt caggtgcaac atccatcgcg gcgatcgcgg
    34321 cttcaatctg cgcttcctgg aagtaatatg gcgcttgcat cagcatggcg actttgtaag
    34381 gacgaggata cgtctggcac tcaaaacgaa cggcgtgtgc aatggcgcga caaatgtcct
    34441 gctccacaac ttgcaaagac atcgccgcgt agttttcact catggcctgc gttgagtaat
    34501 agtaatcatc ctgacttccc gtcatacaag cgatatccgc gtagtctccg ttctgcttaa
    34561 tgctttccag caaaactgac agttcctctt ctgcaaccga atagggtggc gagagaaaca
    34621 cggtgcgcgc caccagctgc ccagccgcag aatgctggcg aatgaactca gccaggattt
    34681 cagcaggttc tggttcggtt accgtatcaa cggggtgacg catcccctgc aaccattgcc
    34741 gcttcgcctc gtcaacttcg ctgactttcg ctaacggcaa ttcatcctga tggttcatct
    34801 tacccattcc ttacgcaggc agtagattgt gtgctgccag atcggcggca atatcttcca
    34861 gccccagacg ctgcaatgtt tcgcgggttg gacaaccaag ctgtgggtcc cagcccattt
    34921 ctttgtagaa catcgtgagc gaagcatgca tatcgtcacg atccattttg tcagtacctt
    34981 cagtaaagac cgggatctgc ggatccttgt cgaaaaccca ggaacagata agatcgtgtt
    35041 cattacgcat atctttggtt tgcatcggtt ttaccgtgta ggcacgatgc aacgtaaaga
    35101 tacgctctgc agctaaatct aatttttcct gagtcatctc ttcgccggtg atcgctttga
    35161 agaatttggc ttcaagcgcc agatcgcccc ggtaattacg gcttttcagc ggcgaaacgg
    35221 tcattggcca gacccagttg cacagagtga cggcgttatg caaacagacc cgcaacagcg
    35281 accactttgc atatttaatt tttgcgtcat tgattggcgt gtagtttttg gtttcatcgt
    35341 aagcatcttc agaaccaaaa agttctttcg ccacttcacg ttgcagtttc aatggcaagc
    35401 cggaaccaat aaagttgata tgggtatgcg tcatgcagtc acggttgaac atacacttaa
    35461 caatggaacc gacctgcgct gacgcttcat tggcatggtg aaccggatag ccaaacggcg
    35521 accagagttt atttttcgcg tagccccagt actctttacc caaattccag cgttctgcga
    35581 tggcatatga accatcagcc aggtgactca gctcacccac acgatgcgcc agacggtagt
    35641 aaaaatcttt aatgaagtta acgtcacccg cttccagttg atcccagcga atttctgcat
    35701 actcttcagc tggcagaaca cgcttgaaca cacctttgct gtaacaataa gtaaagtcgc
    35761 gatgcaactg cccgtagtta caccataggc cgtagtcatc gaacaaattc agaccaacca
    35821 ggttaccaat cacacgacca tcgtctttat cttcaaaatc tttcggcccg ttcgggaaga
    35881 tggtggtatg gacaaagttt gctacacaag tgttaccacc tgtgctgggc acgccaaact
    35941 ctttcacccg aggaatattc atttgggtca tacaacggat cgggcaagag tggcagccgc
    36001 tcatttttac tgtgtatttc tctgccgccg gtcctaagtc aaaaacggat ttataggtac
    36061 gaaagccgac cgtattctga ttgcctggcg gaatttcacc cgtttcaatc ggaccacctt
    36121 cagccgcgcc ccaaaacagc cctttacgtg ctgtccagcg tgacttggga tctgaatact
    36181 ctgcccacga ttgtggcgta cttggcacga catggttgtt attcgcacca ataagttcag
    36241 tcatcatgta atcattgaga cgcttcatct cctgacgatc ggcaatgttg acccctttcg
    36301 tcccttcaac cgcaatcgct ttcaggtttt tcgaacccat tattgcgcca gttcccgcac
    36361 cgccgctgtg gttacggcta ttcaacatgc cagagagagg aataaggttt tccccagcct
    36421 gaccaatagc cgccacacag gtttccgggc tggtcaatcg acaaatttct tccgtcgttg
    36481 cgcgcgtccc ttttccccat aagaaatcgg ctttttccag gctaactttg tcatctttaa
    36541 tcttcagcca taccggtgat ttcgccttcc cttcgataat aatgacgtcg tatccagcga
    36601 atttcatttg cgctgcaaaa aagccaccca tatgggcatc gcgactaaat ttcctttggt
    36661 aaaagtagaa agtgaggtga tatttacgcg agaactacag ggggcaccag atccaggtaa
    36721 tgggccggta gcaaagacta atttattcgc ttcatcgaaa ggtttcgtgc ctagcggtac
    36781 ttcgtcatac ataattttgt agccgaagcc catgccaccg acaaaacttt taaacttact
    36841 ggaatcttcg agggtaatat ttcctgtcgt gagattgact cttaatatat tacctgtcca
    36901 accgttagcc atgatttttt cctttgcaag attcacacag taatatcttt ccactcgata
    36961 atttttaacg cccctgttgg gcaggcgttt gcgcattcac cgcataacac acatttcgag
    37021 gattttttac tttcggtatt tacggtggcc atcatccacg ggcatgccgt agtacaggcg
    37081 ctacagccaa tacaacgttt atgatcgacg gtaatacagc cttctttctg ctgccaggta
    37141 atcgcaccaa tcgggcagac gttcatgcat tgcggctctt tgcattgacg gcaggtgtcc
    37201 gcggtatagt tgagatcgcc atacaggccg ccgccagagc caaccccgtt gtcgccaaag
    37261 aaataattgc gatggatttt gatacgggag aagaatgtcc ctactgagcc atcgttgaag
    37321 ttggtacaag agatttcaca gcggtggcaa cccgtgcatc gcgctcgctg ggtcaccagc
    37381 acccctttgg gtgtatttat caaccctact gtgccgctat caatatcttc ctgtttgcaa
    37441 cccaaaagtg acagtaacgc aggggcaatt gttagaccag caaggccttt tcctgatata
    37501 cgcaagaatt ctaatcgcgt taagccaata tctaatagtg gacgatcaac cgggttcatt
    37561 tatttcgcat cctcttcgca aacacagtgc tgacaattca gcagcctgat attaagcatt
    37621 caactaatac gctgctgact ctgtccccaa caccagatat tttccctaac ccgatagtgt
    37681 atgagaaaag atttaacgcc gataaactaa tcctttatgg ttaggagaaa aaataatgtg
    37741 attattctga gcccttaaca ttgatcgtta tca
//
LOCUS       DAZPCS010000024        37475 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZPCS010000024 DAZPCS010000000
VERSION     DAZPCS010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37475
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.024"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(24..278)
                     /locus_tag="ACHUQ7_001193"
     CDS             complement(24..278)
                     /locus_tag="ACHUQ7_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFR1134694.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            433..1005
                     /gene="yeiP"
                     /locus_tag="ACHUQ7_001194"
     CDS             433..1005
                     /gene="yeiP"
                     /locus_tag="ACHUQ7_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFR1134695.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            1228..2694
                     /locus_tag="ACHUQ7_001195"
     CDS             1228..2694
                     /locus_tag="ACHUQ7_001195"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFR1134696.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERSLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAYISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            2812..3798
                     /gene="yeiR"
                     /locus_tag="ACHUQ7_001196"
     CDS             2812..3798
                     /gene="yeiR"
                     /locus_tag="ACHUQ7_001196"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFR1134697.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            3837..4550
                     /gene="lpxT"
                     /locus_tag="ACHUQ7_001197"
     CDS             3837..4550
                     /gene="lpxT"
                     /locus_tag="ACHUQ7_001197"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFR1134698.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFMVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            4962..5528
                     /gene="mepS"
                     /locus_tag="ACHUQ7_001198"
     CDS             4962..5528
                     /gene="mepS"
                     /locus_tag="ACHUQ7_001198"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFR1134699.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            5709..7265
                     /locus_tag="ACHUQ7_001199"
     CDS             5709..7265
                     /locus_tag="ACHUQ7_001199"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFR1134700.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVHETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPDREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISHPLPLDDFVR
                     WLKKPYTPQW"
     gene            7347..9161
                     /locus_tag="ACHUQ7_001200"
     CDS             7347..9161
                     /locus_tag="ACHUQ7_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFR1134701.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRNRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            9162..10256
                     /locus_tag="ACHUQ7_001201"
     CDS             9162..10256
                     /locus_tag="ACHUQ7_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFR1134702.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            10256..11281
                     /locus_tag="ACHUQ7_001202"
     CDS             10256..11281
                     /locus_tag="ACHUQ7_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1134703.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            11283..12872
                     /gene="yejF"
                     /locus_tag="ACHUQ7_001203"
     CDS             11283..12872
                     /gene="yejF"
                     /locus_tag="ACHUQ7_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFR1134704.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(12876..13220)
                     /locus_tag="ACHUQ7_001204"
     CDS             complement(12876..13220)
                     /locus_tag="ACHUQ7_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFR1134705.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(13553..14749)
                     /gene="bcr"
                     /locus_tag="ACHUQ7_001205"
     CDS             complement(13553..14749)
                     /gene="bcr"
                     /locus_tag="ACHUQ7_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HFR1134706.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVL
                     VWLSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVL
                     SYMLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVR
                     RIGALNMFRSGLWIQFIMAAWMVISAPLGLGFWSLVVGVAAFVGCVSMVSSNAMAVIL
                     DEFPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYA
                     SRPKKR"
     gene            complement(14777..15472)
                     /gene="rsuA"
                     /locus_tag="ACHUQ7_001206"
     CDS             complement(14777..15472)
                     /gene="rsuA"
                     /locus_tag="ACHUQ7_001206"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFR1134707.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            15622..17382
                     /locus_tag="ACHUQ7_001207"
     CDS             15622..17382
                     /locus_tag="ACHUQ7_001207"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFR1134708.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDNPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            17507..17791
                     /gene="rplY"
                     /locus_tag="ACHUQ7_001208"
     CDS             17507..17791
                     /gene="rplY"
                     /locus_tag="ACHUQ7_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFR1134709.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(17930..18937)
                     /gene="yejK"
                     /locus_tag="ACHUQ7_001209"
     CDS             complement(17930..18937)
                     /gene="yejK"
                     /locus_tag="ACHUQ7_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFR1134710.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            19119..19346
                     /locus_tag="ACHUQ7_001210"
     CDS             19119..19346
                     /locus_tag="ACHUQ7_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFR1134711.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFASALQSSINEDKAH"
     gene            19366..21126
                     /gene="yejM"
                     /locus_tag="ACHUQ7_001211"
     CDS             19366..21126
                     /gene="yejM"
                     /locus_tag="ACHUQ7_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFR1134712.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQIKRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            21201..21277
                     /locus_tag="ACHUQ7_001212"
     tRNA            21201..21277
                     /locus_tag="ACHUQ7_001212"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21235..21237,aa:Pro,seq:ggg)
     gene            complement(21380..23971)
                     /gene="yejO"
                     /locus_tag="ACHUQ7_001213"
     CDS             complement(21380..23971)
                     /gene="yejO"
                     /locus_tag="ACHUQ7_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HFR1134713.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGAVFAAIEKGGLLEVKEGG
                     FALAVDQKTGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            24291..24938
                     /gene="narP"
                     /locus_tag="ACHUQ7_001214"
     CDS             24291..24938
                     /gene="narP"
                     /locus_tag="ACHUQ7_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFR1134714.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGEGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(25149..26201)
                     /gene="ccmH"
                     /locus_tag="ACHUQ7_001215"
     CDS             complement(25149..26201)
                     /gene="ccmH"
                     /locus_tag="ACHUQ7_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFR1134715.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(26198..26755)
                     /gene="dsbE"
                     /locus_tag="ACHUQ7_001216"
     CDS             complement(26198..26755)
                     /gene="dsbE"
                     /locus_tag="ACHUQ7_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFR1134716.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(26752..28695)
                     /gene="ccmF"
                     /locus_tag="ACHUQ7_001217"
     CDS             complement(26752..28695)
                     /gene="ccmF"
                     /locus_tag="ACHUQ7_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFR1134717.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(28692..29171)
                     /gene="ccmE"
                     /locus_tag="ACHUQ7_001218"
     CDS             complement(28692..29171)
                     /gene="ccmE"
                     /locus_tag="ACHUQ7_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFR1134718.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(29168..29377)
                     /gene="ccmD"
                     /locus_tag="ACHUQ7_001219"
     CDS             complement(29168..29377)
                     /gene="ccmD"
                     /locus_tag="ACHUQ7_001219"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFR1134719.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(29374..30111)
                     /gene="ccmC"
                     /locus_tag="ACHUQ7_001220"
     CDS             complement(29374..30111)
                     /gene="ccmC"
                     /locus_tag="ACHUQ7_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFR1134720.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     IMVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(30153..30815)
                     /gene="ccmB"
                     /locus_tag="ACHUQ7_001221"
     CDS             complement(30153..30815)
                     /gene="ccmB"
                     /locus_tag="ACHUQ7_001221"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFR1134721.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(30812..31435)
                     /gene="ccmA"
                     /locus_tag="ACHUQ7_001222"
     CDS             complement(30812..31435)
                     /gene="ccmA"
                     /locus_tag="ACHUQ7_001222"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFR1134722.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQTLLWIGHQPGIKTRLTALENLHFYHR
                     DGDAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(31448..32050)
                     /gene="napC"
                     /locus_tag="ACHUQ7_001223"
     CDS             complement(31448..32050)
                     /gene="napC"
                     /locus_tag="ACHUQ7_001223"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFR1134723.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(32060..32509)
                     /gene="napB"
                     /locus_tag="ACHUQ7_001224"
     CDS             complement(32060..32509)
                     /gene="napB"
                     /locus_tag="ACHUQ7_001224"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFR1134724.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(32506..33369)
                     /gene="napH"
                     /locus_tag="ACHUQ7_001225"
     CDS             complement(32506..33369)
                     /gene="napH"
                     /locus_tag="ACHUQ7_001225"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFR1134725.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(33356..34051)
                     /gene="napG"
                     /locus_tag="ACHUQ7_001226"
     CDS             complement(33356..34051)
                     /gene="napG"
                     /locus_tag="ACHUQ7_001226"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFR1134726.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(34058..36544)
                     /gene="napA"
                     /locus_tag="ACHUQ7_001227"
     CDS             complement(34058..36544)
                     /gene="napA"
                     /locus_tag="ACHUQ7_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HFR1134727.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNSRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(36541..36804)
                     /gene="napD"
                     /locus_tag="ACHUQ7_001228"
     CDS             complement(36541..36804)
                     /gene="napD"
                     /locus_tag="ACHUQ7_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFR1134728.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(36794..37288)
                     /gene="napF"
                     /locus_tag="ACHUQ7_001229"
     CDS             complement(36794..37288)
                     /gene="napF"
                     /locus_tag="ACHUQ7_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFR1134729.1"
                     /translation="MKIDASRRGILTGCWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            37397..>37475
                     /gene="yojO"
                     /locus_tag="ACHUQ7_001230"
     CDS             37397..>37475
                     /gene="yojO"
                     /locus_tag="ACHUQ7_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YojO"
                     /protein_id="HFR1134730.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFA"
BASE COUNT         9137 a        10141 c         9267 g         8930 t
ORIGIN      
        1 tgcttactgc ggattaaaga tgtttaaggc gcggtcagca tttccagatc gataagccag
       61 gtcatggcct gctgacgcga gttaccgcac atttcagcgc tggcttgtaa gcctgcacag
      121 actttcggtc gcagtggcga ggtgaaaatt ttacagcgtt gctgttcatc aagctgaatg
      181 cagggcgtat tggcgggctt gccatctggc ataccgggaa tagggctgga aattgaaggg
      241 gcagtgcaac acgccccgca acccggacgg cattccatac gttgttctct taaggttaac
      301 caccggattg atgagcgcgc acagtaccac cttttttgca ccagcaaaag tgcgaatacc
      361 acttgcccga aaggcccgtc gcgagtactt tgtcgcgata tttttgacat tttcgactac
      421 aggaattttt cgatgccaag agcgaacgaa attaaaaaag gtatggtact gaattacaac
      481 ggcaaactgc tgctggctaa ggatattgat attcagtcgc ccactgcccg cggtgccgct
      541 acgctgtaca aaatgcgttt ttctgatgtc cgtaccgggc tgaaagtaga agagcgtttc
      601 aaaggtgatg atatcgttga caccgtgacg ctgacccgcc gttacgttga cttctcctat
      661 gtcgatggca acgaatatgt ctttatggat aaagaagact ataccccgta taccttcacc
      721 aaagatcaga ttgaagaaga gttgctgttt atgccagagg gcggcatgcc ggacatgcag
      781 gtgctgacct gggacggtca actgctggcg cttgagctgc cgcagaccgt tgatctggaa
      841 atcgttgaaa ccgcaccagg catcaaaggg gcatctgcca gcgcccgtaa caaaccggcg
      901 acattgagca ctggtctggt gattcaggta ccagaatact taagcccggg cgaaaaaatt
      961 cgtatccata tcgaagaacg ccgttatatg gggcgtgctg actaacttca gccgcatgca
     1021 gaaaagggat agctcaggct gtcccttttt taatttatta taccaatctt ctattttgcg
     1081 cttcctgaac accttacgcc ctcaattttc actcgttgat tgatcgccct cacacttcat
     1141 cgcattaaca atccagacca atttcaattg ctgtcatata actttacact gtcattgtta
     1201 atttatcgtt actaagacgt gactcctatg aagacaattg cctccgttac gctcccgcat
     1261 catgtacacg ctccacgcta cgatcgccag cagttgcaat cacgtatcgt tcattttggc
     1321 tttggcgcct ttcaccgcgc tcatcaggcg ttactgaccg atcgtgtgct gaatgcccag
     1381 ggcggcgact gggggatctg tgaaatcagc ttgttcagcg gtgatcaact gatgagccag
     1441 ctccgcgcac agaaccattt atataccgtg ctggagaaag gtgcggacgg caatcaggtg
     1501 ataattgtcg gtgccgttca cgaatgcctt aatgcaaaac tggattcctt agcggcaatt
     1561 attgagaaat tttgcgagcc acaggtggca attgtttccc tgacgattac cgaaaaaggc
     1621 tattgtattg acccggccac cggtgcactc gacaccagta atccgcggat tattcacgat
     1681 ctacaaaccc ctgaagaacc tcactccgca ccgggtattc tcgtcgaagc actgaaacgc
     1741 cgccgtgagc gcagccttac accgtttacc gtgctctcct gcgacaatat tcccgacaat
     1801 ggccatgtgg tgaaaaacgc ggtgctgaga atggcagaaa aacgttcgcc agaactcgcc
     1861 gggtggataa aagaacacgt cagttttccg ggaaccatgg tcgaccgcat tgttccggct
     1921 gcaaccgacg aatcactggc agaaatcagc cagcatctgg gggtaaatga tccctgcgcg
     1981 attagctgcg aaccgtttat ccagtgggtg gttgaagata acttcatcgc tgggcgtcct
     2041 gcctgggaag tcgcaggtgt acaaatggtg aatgatgtcc tgccatggga agagatgaaa
     2101 ctgcgtatgc ttaatggcag ccactctttt ctcgcttatc tgggttacct ctcaggattc
     2161 gcctatatca gtgattgcat gcaggatcgc gtatttcgct atgctgccag aacattaatg
     2221 ctggatgagc aagcgccgac actgcaaatt aaagatgtcg atttaacaca atatgcggat
     2281 aagttaattg cacgttttgc taatccgtcg ctgaaacata agacctggca aatcgcgatg
     2341 gatggcagcc agaaattacc gcaacgtatg ctggcaggta ttcgcataca tctggggcgt
     2401 gaaacggact ggtcgttgct ggcattaggc gttgctggct ggatgcgtta cgtcagcggc
     2461 gttgatgatg ccggaaatgc cattgatgtt cgcgatccgc ttagcgataa aattcgcgaa
     2521 cttgttgcgg gcagcagcag tgaacaacgc gtaaccgccc tgctttccct gcgtgaagtt
     2581 ttcggtgatg atctgccaga taacccgcat tttgtgcagg ccatcgaaca agcctggcaa
     2641 caaatcgtac aattcggcgc acatcaggcg ctattaaaca ccctcaaaat ttaacgattt
     2701 ctgcggttaa agcggatgaa gctcaccttc gtccgctctc cccttctctt ttctgccttt
     2761 tttagccagg attaacgctc agttaactta ccagagtatt gcggagccgt tgtgaccagg
     2821 accaacctca tcaccggttt tctcggcagc gggaaaacca cgtcgattct tcatctgtta
     2881 gcccataaag atcccaacga aaaatgggcg gtactggtta atgaatttgg ggaagtcgga
     2941 attgatggtg ctttgctcgc cgatagcggc gcattgctga aagagatccc cggcggctgc
     3001 atgtgctgcg ttaatggttt acccatgcag gtagggttga ataccttact gcgtcaggga
     3061 aaaccagacc gcttgttgat agagccgacc gggctgggcc atccgaaaca gatcctcgat
     3121 ctgttaaccg caccagtcta tgaaccgtgg atagatctgc gcgccacctt gtgcattctc
     3181 gatccacgcc tgctgctgga cgaaaaaagc gccagcaatg aaaacttccg tgaccagctg
     3241 gctgccgcag acatcattgt cgccaataaa tccgaccgtg cgacgcccga aagtgagcaa
     3301 gcgctacagc gttggtggca gcaaaatggt ggcgatcgac aattaattca cagtgagcat
     3361 gggaaagttg acggtcatct tctggatttg ccgcgtcgca atttagccga gttgcccgcc
     3421 agcgccgcgc attctcatca gcatgtcgtg aaaaaagggt tagcagcgtt aagcctgcca
     3481 gagcatcaac gctggcgtcg cagtctgaac agcgggcaag gatatcaggc ctgcggctgg
     3541 atattcgacg ctgatacggt attcgacacc attggcattc tggaatgggc gcgacttgca
     3601 ccggtggaac gcgtcaaagg cgtgctgcgt attcccgaag ggctggtacg aatcaaccgt
     3661 cagggcgatg acctgcacat tgaaacgcaa aacgttgcgc caccggacag ccgtattgag
     3721 ctgatttcca gcagcgaagc tgactggaat gccttgcaga gcgcgctgtt gaagcttcgt
     3781 ttagcgacta ccgcgtaagg ttgcctgcgt ttttcagtaa gataattaga gaaaatatga
     3841 ttaaaaattt gccgcaaata gtgttgttga atattgtcgg ccccgcgctg tttctttcct
     3901 ggtatattcc cgttaatcat ggattctggt tgccgattga tgcggatatt ttttatttct
     3961 ttaatcagaa actggtcgaa agtaaggcct ttttatggct ggttgcattg actaacaatc
     4021 gcgccttcga cggttgttca ctgctggcga tgggtatgtt gatgctgagt ttctggctga
     4081 aagaaaacgc ccctggcaga cgacgtatcg tgattatggg tctggtcatg ctattaactg
     4141 cagtggtatt aaaccagctg ggtcaggcat taattcctgt aaaacgggcc agcccaacat
     4201 tgacttttac cgatattaac cgcgtcagcg aactgctctc tgttcccacg aaagatgcct
     4261 cacgagatag ctttcccggc gatcacggca tgatgctgct tattttttcg gcattcatgt
     4321 ggcgttattt cggcaaagtt gcaggcctta tcgcccttat tatttttatg gtttttgcat
     4381 ttcccagagt aatgattggc gcacactggt ttactgacat cattgtcggt tcgatgaccg
     4441 tgatattgat cggtttgccc tgggtgttgc tgacgccatt aagtgatcga ttaatcacct
     4501 tttttgacaa atcactacca ggaaaaaaca aacatttcca aaacaaataa ctcacagtaa
     4561 ttaacatcat cagggttatt tttatagtga ggataatcct gatgatgcgc accgtgcttt
     4621 catctatcga acgcaaaaat cattctctaa gtaaatgaat ggattgcatg cgtttcactc
     4681 aattgtactt taattgacca accccgctta ttaactttct gtatcacttt ttcttataaa
     4741 aaatcatgta aaaccgctcg ccaagaccgc accaatcggg taatctcgaa ctcgttttgc
     4801 ctcggcggta gattatcctc acagcatata attttgtgcg ttagtccaca gatttggcct
     4861 taaggaattg tttcaacatg cccaggtaat tagtctcgtg tcgcttggca tttttttata
     4921 acgatatttg tcgttaagga cttcaaggga aaacaaacaa catggtcaaa tctcaaccga
     4981 ttttgagata tatcttgcgc gggattcccg cgattgcagt agcggttctg ctttctgcat
     5041 gtagtgcaaa taacaccgca aagaatatgc atcctgagac acgtgcagtg ggtagtgaaa
     5101 catcatcact gcaagcttct caggatgaat ttgaaaacct ggttcgtaat gtcgacgtaa
     5161 aatcgcgaat tatggatcag tatgctgact ggaaaggcgt acgttatcgt ctgggcggca
     5221 gcactaaaaa aggtatcgat tgttctggtt tcgtacagcg tacattccgt gagcaatttg
     5281 gcttagaact tccgcgttcg acttacgaac agcaggaaat gggtaaatct gtttcccgca
     5341 gtaatttgcg tacgggtgat ttagttctgt tccgtgccgg ttcaacggga cgccatgtcg
     5401 gtatttatat cggcaacaat cagtttgtcc atgcttccac cagcagtggt gttattattt
     5461 ccagcatgaa tgaaccgtac tggaagaagc gttacaacga agcacgccgg gttctcagcc
     5521 gcagctaata aaccgtttgg atgcaatccc ttggctatcc tgacgagtta actgaaagca
     5581 ctgcttaggc agtgcttttt tgttttcatt catcagagaa aatgatgttt ccgcgtcttg
     5641 atccaggcta tagtccggtc attgttatct tttaaatgtt gtcgtaattt caggaaatta
     5701 acggaatcat gttcatacgc gctcccaatt ctggacgtaa gctcctgctt acctgcattg
     5761 tagcaggcgt gatgattgcg atactggtta gctgccttca gtttttagtg gcctggcata
     5821 agcacgaagt caaatacgac acactgatta tcgacgtaca aaagtatctc gatacctatt
     5881 ttgccgacct gaaatccact actgaccggc ttcagccgct gaccttagat acctgccagc
     5941 aggctaaccc cgaactgacc gctcgcgcgg cgtttagcat gaatgtccgt acgtttgtgc
     6001 tggtgaaaga taaaaaaaca ttctgttcat ctgcgactgg tgagatggac attccactca
     6061 atgaattgat tccggcgctc gacattaata aaaacgtcga tatggcgatc ttacccggca
     6121 cgccgatggt gccgaacaaa cccgcaatcg tcatctggta tcgcaaccct ttgctgaaaa
     6181 atagcggtgt gtttgccgct ctgaatctca acctgacgcc ttctctcttt tatagttcac
     6241 ggcaggaaga ttacgatggc gtcgccctca ttattggcaa tactgcgcta tctacctttt
     6301 cttcacgttt gatgaacgtt aatgaattaa ccgacatgcc ggtccatgaa actaaaattg
     6361 cgggcattcc tctgaccgtt cggctttatg cggatgactg gacatggaac gatgtgtggt
     6421 acgcattttt actgggcggc atgagtggaa ctgtcgttgg cctgctttgc tattacctga
     6481 tgagcgtacg tatgcgcccc gacagagaaa tcatgaccgc catcaagcgc gaacaatttt
     6541 acgtggcgta tcaaccggtg gtggatacac aagctttgcg ggtaacgggc ctggaagtac
     6601 tgctacgctg gcggcatcct gtagcgggag aaattccccc ggatgccttc attaactttg
     6661 ccgaatcgca aaagatgatt gtgccgctga ctcagcacct gtttgagtta attgcccgcg
     6721 atgccgcaga attagaaaaa gtactgccgg taggcgtcaa atttggcatt aacattgcgc
     6781 cggaccatct gcacagcgaa agctttaaag cagatatcca gaaactgctc acttccctgc
     6841 ccgcacacca tttccagatt gtgctggaaa ttaccgagcg cgatatgctg aaagagcaag
     6901 aagccacaca actcttcgcc tggctgcact cggtcggcgt agaaattgct attgatgact
     6961 tcggcaccgg gcacagcgcg cttatctatc ttgagcgttt tacgctcgat tatctgaaaa
     7021 ttgaccgtgg atttatcaac gccatcggta cggaaacgat cacttcaccc gtacttgacg
     7081 cggtgctgac gctggcgaaa cgtctcaata tgctgacagt tactgaaggg gtcgaaacgc
     7141 cggaacaagc gcgatggcta agcgaacgcg gcgttaattt catgcaaggc tactggatta
     7201 gtcacccgtt accgctggac gattttgttc gctggctgaa gaaaccgtat acgccgcagt
     7261 ggtaaggtgt gcttacgtcc cttattattc atagtgaaag catgccggat tgcggctaat
     7321 gatgagtaaa aggaaatccg ttgcagatga ttgtgcgcat actgctgctg tttatcgctc
     7381 tgttcacctt cggtgcgcag gcgcaggcta tcaaggaaag ctataccttt gccgtgctgg
     7441 gcgaaccccg gtacgcgttt aatttcaacc attttgatta tgtgaacccc gccgcgccaa
     7501 aaggtgggca gataacgttg tcagccctcg gtaccttcga taatttcaac cgctatgcac
     7561 tgcgcggcaa cccgggcgca cgcaccgagc agctgtacga cacgttattt acgacttccg
     7621 atgacgaacc aggcagttat tacccgctga ttgctgaaag tgcacgctat gctgacgatt
     7681 attcctgggt ggaggtcgct attaatccgc gcgcccgttt tcatgatggt tctcccatta
     7741 ctgcccgcga tgtagagttt acttttcaaa aatttctgac cgaaggcgtg ccgcaatttc
     7801 gtctggtcta caaaggcacc accgtcaaag ccattgcgcc gttaaccgtg cgcattgagt
     7861 tagctaaacc cggcaaagaa gatatgttga gtctgttttc gctgccggta tttccagaaa
     7921 agtactggaa ggatcacaaa cttagcgacc cgctcgccac gcctccgctt gccagtggtc
     7981 cgtaccacgt tacgtcctgg aaaatggggc aaaatattgt ctattcccgc gtgaaagatt
     8041 actgggcagc aaacttaccg gtaaaccgtg gacgctggaa tttcgacacc attcgctacg
     8101 attattacct cgatgataat gtcgcctttg aagcgtttaa agcaggtgcc tttgatttgc
     8161 gtatggaaaa cgacgctaaa aactgggcca cgcgttatac cggtaaaaat ttcgataaaa
     8221 aatacatcat caaagatgag caaaagaacg aatcagccca ggatacgcgc tggctggcgt
     8281 ttaatatcca acgtgcggta ttcagcgatc gccgggtgcg ggaagcaatc actctcgcct
     8341 ttgactttga atggatgaac aaggcgttgt tttacaacgc ctggagtcgc acgaacagtt
     8401 attttcaaaa taccgaatac gcggccagaa attaccccga cgccgcggag ctggtgcttc
     8461 tggcaccaat gaaaaaagat ctaccgccag aagtcttcac acaaatctac cagccgccgg
     8521 tatctaaagg cgatggctac gatcgtgaca acctgttaaa agccgacaaa cttctcaacg
     8581 aagcgggctg ggtgctgaag ggtcagcaac gcgttaatgc cacaacgggt cagccgctca
     8641 gctttgaatt attgcttccc gcaagcagca atagtcagtg ggtattgccg ttccagcaca
     8701 gcctgcaacg gctgggtatc aatatggaca ttcgcaaggt ggataactcg caaatcacta
     8761 accgcatgcg caatcgcgac tatgacatga tgccgcgact atggcgggcg atgccgtggc
     8821 ccagttccga tttacagatt tcctggtcat cggaatatat caattccact tataatgccc
     8881 ccggcgtgca aagcccggtt atcgactcac tgatcaacca aattattgcc gcgcagggaa
     8941 ataaagaaaa attactgccg ttggggcgag cactggatcg cgtattaacg tggaattatt
     9001 acatgctgcc aatgtggtac atggcggaag accgtctcgc ctggtgggat aaattctccc
     9061 aacccgctgt acgccctgtt tacagcctgg gtatcgatac ctggtggtat gacgttaata
     9121 aagcggccaa actgccgtca gccagacaac agggagagta gatgggcgct tacctgattc
     9181 gccgtctgtt gctggtgatc ccaacgttat gggctattat cactatcaac tttttcatcg
     9241 tgcaaattgc gcctggcggt ccggtcgatc aggccatcgc cgccattgag tttggtaatg
     9301 ccggagtatt acccggcgca ggcggtgaag gtgttcgtgc cagccatgcg caaacgggtg
     9361 tcggcaatat cagcgacagt aattaccgtg gcggacgcgg attagatcca gaagtgatcg
     9421 ctgagatcac tcatcgctac ggtttcgata agccgatcca cgaacgttac ttcaaaatgc
     9481 tctgggacta catccgcttt gattttggtg atagcctgtt tcgcagcgcc tcggtgctga
     9541 cgctgattaa agacagtctg ccggtttcca tcaccctcgg attgtggagc acgctgatta
     9601 tctatctggt gtcgattccg ttaggcattc gcaaagctgt ttataatggg agtcgctttg
     9661 acgtctggag tagcgcattt atcatcatcg gctacgccat tccggccttt ttgtttgcca
     9721 tcctgctgat tgtcttcttc gcgggcggca gctatttcga cctgttccct ctacgcggcc
     9781 tggtttccgc taactttgat tcgctgccgt ggtatcagaa aatcaccgat tatctgtggc
     9841 atatcacgct accggtgctg gcgacagtga ttggtggctt tgcggcgctg accatgctga
     9901 ccaaaaactc attccttgat gaagtgcgca agcaatacgt ggtgaccgcg cgtgcgaaag
     9961 gggtaagtga gaaaaacatt ctctggaaac atatgttccg caacgccatg ctgctggtga
    10021 ttgccggttt tccggcgacg tttatcagca tgttttttac cggctcgctg ctgattgagg
    10081 tgatgttttc actcaatggt cttggcttac tgggctacga agcgaccgtc tcgcgcgatt
    10141 atcctgtaat gtttggtacc ttgtatattt tcaccctgat tggcctgctg ctgaatattg
    10201 tcagtgatat cagctatacg ctggttgatc cgcgtataga ttttgaggga cgttaatgtc
    10261 gcgactcagc cccgtcaatc aggcccgttg ggcgcgtttt cgtcataacc gtcgcggcta
    10321 ctggtcgtta tggattttcc tcgttttgtt tggtttgagt ttgtgttctg aacttatcgc
    10381 taacgataaa ccgttgctgg tgcgttatga cggcagttgg tatttcccgt tgttgaaaaa
    10441 ctacagcgaa agcgattttg gcggcccgct ggcaagtcag gctgattatc aggacccgtg
    10501 gctgaaacaa cggctggaaa ataacggctg ggtactgtgg gcaccgattc gctttggtgc
    10561 taccagtatc aactttgcta ccgataagcc cttcccttct ccaccctccc ggcaaaactg
    10621 gctgggaacg gatgcaaacg gcggcgatgt gctggcacgc attctctatg gcacgcggat
    10681 ctcggttctg tttggcctga tgctgactct ctgttccagc gtgatgggcg tgctggcggg
    10741 ggcgctacaa ggctattacg gcggtaaagt cgatctctgg gggcaacgct ttattgaagt
    10801 atggtcgggg atgccgacgc tgtttttgat tattttactt tccagcgtcg tacagcctaa
    10861 cttctggtgg ctgctggcaa ttactgtctt gtttggctgg atgagtctgg tcggcgtggt
    10921 gcgggcggag tttttacgta ctcgtaattt cgactacatt cgtgcggcac aggcgcttgg
    10981 cgtcagcgat cgcagtatca tcctgcgtca tatgttgcct aatgccatgg tcgcgaccct
    11041 caccttttta ccgtttattt tatgtagttc gataaccacc ctgacctcgc tcgatttcct
    11101 cggcttcggt ctaccgctcg gttcaccgtc actcggtgaa ctgctgttac aagggaaaaa
    11161 taaccttcag gccccgtggc ttgggatcac cgccttcttg tcggtggcga tattattgtc
    11221 tttgctgatc tttattggtg aagccgtccg cgacgcattt gatcctaata aggcggtgta
    11281 gcatgacgca aactctgtta gcgattgaaa atttgtcggt gggttttcgc catcagcaaa
    11341 ccgtacgtac agtagtcaat gatgtttcac tacagattga ggctggcgaa acgctggcgc
    11401 tggtgggtga gtcaggttca ggcaaaagcg ttaccgcgct gtcaatttta cgcctgctcc
    11461 cttccccgcc ggttgaatat ctctccggcg atattcgttt tcatggcgaa tcgctgcttc
    11521 atgccagcga tcaaacgttg cgcggtgtac gcggtaataa gatcgccatg atttttcagg
    11581 aaccgatggt gtcgttaaat ccattgcata ccctggaaaa acagctttat gaagtgcttt
    11641 cactccaccg cgggatgcgt cgggaagcgg ctcgtggcga aattcttaac tgccttgatc
    11701 gcgtcggtat ccgccaggcg gcaaaacggc tgacagatta tccgcatcag ctctccggcg
    11761 gcgaacgcca gcgggtgatg attgcgatgg cgctgttaac gcgaccggaa ttattaattg
    11821 ccgatgaacc gaccaccgca ctggacgtct ctgtccaggc gcagatttta cagctgttgc
    11881 gcgaactgca aggcgagctg aatatgggca tgctgtttat tactcataac ctcagcattg
    11941 tcagaaaact ggcccaccgc gtggcggtaa tgcaaaacgg tcgctgtgtc gagcaaaata
    12001 acgccgctac gctatttgcc tcacccactc atccttacac acaaaagcta ctcaacagtg
    12061 aaccgtcagg cgatccggta ccgttgccag aacctgcctc aacgttgctg gatgttgaac
    12121 agcttcaggt tgccttcccc attcgcaaag ggattttgaa gcgcattgtg gatcataatg
    12181 tggtggtgaa aaacatcagt tttacgctac gggcgggtga aacactgggt ttagtgggcg
    12241 agtctggttc cgggaaaagc acgacgggac tggcgctgct gcgactgatt acctctcagg
    12301 gcagcatcgt ctttgacggt cagccactgc aagatttaaa tcgccgccag ctgttaccta
    12361 ctcgtcatcg tattcaggtg gtatttcagg atccaaactc ctcgctcaac ccacgactca
    12421 acgttttgca gattattgag gaaggcttac gggttcacca gccgacgctt tctgccgcac
    12481 aacgcgaaca acaagtgata gccgtgatgc atgaagtggg attagatcct gaaacacgcc
    12541 accgttatcc ggcggagttc tctggtggtc agcgacaacg tattgcgatt gccagggcat
    12601 taattcttaa gccctcgctg atcatacttg atgaaccgac atcatcactc gacaaaacgg
    12661 tacaggcgca aatattgacg cttttgaaat cattgcaaca aaagcatcaa ctggcctatt
    12721 tgtttatcag ccacgatttg cacgttgtcc gcgcgttatg tcatcaggtt atcgtactgc
    12781 gacaagggga agtagtggaa caaggaccgt gcgcgcgcgt gtttgccaca ccgcagcagg
    12841 agtatacgcg tcagttactg gcgttgagct gacgcttaaa aaggattgta gtttgaaaag
    12901 ggttcggcaa ttgccacacc aaaatttttc aatcggcatg ttgcagcaaa ctcgtcctgg
    12961 cgatttacaa acaggcacgg ctcaccttca cactccaaca ccgaacatgg aacttcgatg
    13021 tcgcttaacg cctggcttag tttatacatc accgaccatg cattatcacc atccgggcta
    13081 agcaatttaa gcgccaccag gctgttgtca ccgcacggtc cattttgagg aatcggttca
    13141 accttcgaac ctgcgaaacc agcagaccag cgatagttct gcggcagtcg atggacgatt
    13201 gagagttgta atgaagtcac ttattttccc cggaagtaca ttcacttcac aatttgttta
    13261 catcaatttt aacacatcat caacaatccg tctttataca gatcgtaaag attgatgcct
    13321 cgcctgcgtg gcctctatgg ctctgattta agtaatatct gcgggcgtgg tcacgtttgc
    13381 ggggctatat gtacccgttt ctgcgatcta actcaacctt tttaactaca atgatgtgac
    13441 tttttacata aattgatttt acataaaata aacatatatc ggggaaatga tagatttgtg
    13501 gttgacatgc atcaacaaag gaagcctttt accttcctcg ttgtgcaata gatcaccgtt
    13561 ttttcggccg actggcgtac agacagaaga gaatggagct ggttgcgcag aatgcaattg
    13621 accaaatcat cggccatgca gagttaaagg tcgcaagaga aagcaatgcg ccaacaattg
    13681 cccctatgcc aaaacggaag gttcctgcca gcgaagatgc cgttcccgcc atatggggaa
    13741 actcatcaag aatgaccgcc atcgcattgg atgacaccat cgacacgcag cccacaaacg
    13801 ccgcaacgcc aaccaccagc gaccagaatc ccagccccag cggcgcactg atgaccatcc
    13861 acgcagccat aataaattgt atccacaacc ccgagcggaa catatttaac gcgccaatgc
    13921 ggcggacgaa gcggctgtta aagatggtca tcacgaacag aaaaacaatg tttagcgcaa
    13981 agtaataacc aaagttttcc ggcgcgacgt ggttaatttc aatataaaca aacggtccgg
    14041 cgcttaagaa tgagaacatc ccggcaaagc tgaaaccact ggcaagcatg tagctcagga
    14101 cacgtttatg gcggaacagc gccgcaaagt taccaatagt ggtacgaatg tgaaatggct
    14161 gacgacgctc cggtggtaag gtttctttaa tcaggaagaa aatcattgcc gaagccagaa
    14221 tcgccgctaa tgccaggatc cagaagatgt aatgccagct cagccacacc agcacccagc
    14281 cgccaactat cggtgccatc agcagtgcaa tggtcgtcac cagcatgaca aacgacatca
    14341 tccgcgagaa ctcttctttc gggtaaatat cacgcatcaa agcgttgata accacgctgg
    14401 ccgcagccgc agccgcagcc agcccgtgga agaaacgcat cacaatcagc tgatcgatgg
    14461 tttgtgccaa cgcacacgcc accgcggcgg cggcaaacac cagcgtcccg ccaagaacca
    14521 ccggcttacg cccgaagctg tctgccatcg gcccgtagat taactgcccc aacgcaaagc
    14581 ccagaatata agtactgagg gtcatctgcg tactgcccgc cgatacgcca aactgcgctg
    14641 aaattaccgg tagcgcgggc agatacatat caatcgacag cggcatcaac atggccagca
    14701 ggccaaggat aaaaacaata gcaaacgacg aatgctgtcg ggtggtcaca acgggctcct
    14761 gaaagtcatt gaaaagttag acgacgctgg caatttcttc ttcagttaac ggacgatatt
    14821 caccgggggc taaatcagca tccagcgtaa taccgccaat acgttcacga tgcagctcaa
    14881 ccacgtggtt acccacggcg gcgaacatgc gtttcacctg atgataacgc ccttcgctga
    14941 tggtcagacg aacctgcgtt ggggtaatca cttcaagcac cgccggctta gtgagatctt
    15001 tttcgttatg cagctgcacg cctttagcaa attgctctgc cgtatcgtca gctacaggtg
    15061 attccagtgt caccagatag gtcttctcgc aatgatggcg cggagaagta atgcggtgcg
    15121 accactgacc atcatcagtc atcagcacca gaccggtggt atcgatatcc agacgtcctg
    15181 cggcgtgcag tttccacgct accggttcat ccagaaaata gagcaccgtc gggtgatccg
    15241 ggtcgtccgt ggagcaaaca tagccctgag gcttattgag catgaagtaa cgtggaccgt
    15301 gttgctgcgc cagcgggttg ccatcgtaag cgacatcatg ttcagggagc agtttgaacg
    15361 ctgcattacg aacgatttcg ccatcgacgg tgacacgatt gccgcggatt tcacgcccgg
    15421 caatagcacg gctaacgccg agttgctgtg cgataaattt atcaagtcgc atgtgtgtga
    15481 ttttgcctgt aaaaaacgga ggtcgggtaa tgacccgaaa atctgaactg ttgtctgccc
    15541 agtatagcgg tctaataact tccctcaagg gaaaaagaac cgtggcatac tattagcaga
    15601 ataatctacc tacgcgaaac catgattttt acactccgcc catatcagca agaagccgtg
    15661 gatgccacgc tcaaccattt tcgtcgtcat aaaacccctg ccgttatcgt gctgcccacc
    15721 ggcgcaggta aaagcctggt gatagcggaa ctggcacggc tggcgcgtgg tcgcgtactg
    15781 gtgctggcac acgttaaaga actggtggcg caaaaccatg caaagtatca ggcgctgggg
    15841 ctggaagccg atatttttgc cgccgggcta aagcgcaaag agagccacgg taaagtggta
    15901 tttggcagcg ttcagtcggt cgcccgtaat cttgatgcct ttcagggtga attttcgctg
    15961 ttgattgtcg atgaatgcca ccgtattggt gacgatgaag agagccagta tcagcaaatc
    16021 ctcactcacc tgactaaagt gaatccccac ttacgcctgc tggggctgac tgccacgcct
    16081 tttcgattgg gcaaaggctg gatctaccag ttccattatc acggcatggt acgcggcgat
    16141 gagaaagccc ttttccgtga ctgcatttat gagctgccgc tgcgttatat gattaaacac
    16201 ggctatctga cgccgccaga acgactggat atgccagtag tgcaatacga tttcagccgc
    16261 ttgcaggcac agagtaacgg gctgttcagc gaagctgatc tcaaccgtga gctgaaaaaa
    16321 cagcaacgca ttacgccaca tatcatcagc cagatcatgg agttcgccga aaagcgcaaa
    16381 ggggtaatga ttttcgccgc caccgttgaa cacgcaaaag agattgtggg attactgcct
    16441 gccgaagatg ctgcactgat tactggcgac aaccccggcg ctgagcgcga tgtgttaatt
    16501 gaagatttta aagcccagcg ttttcgctat ctggtcaacg tcgcagtact gaccaccgga
    16561 tttgacgccc cgcacgtcga tcttatcgcc attctgcgcc ctaccgaatc agtgagtctt
    16621 taccaacaaa ttgtcgggcg cggtctgcgt ctcgctccgg gcaagactga ttgtttaatt
    16681 cttgattatg cgggtaatcc tcacgatctc tacgcgccgg aagttggtac accgaaaggc
    16741 aaaagtgaca acgttccggt acaggttttc tgccctgcct gcggttttgc caacaccttt
    16801 tgggggaaaa cgaccgccga cgggacattg attgaacact ttggtcgccg ctgtcaggga
    16861 tggtttgaag atgacgacgg tcatcgcgaa cagtgtgact tccgtttccg ttttaaaaat
    16921 tgcccgcaat gtaacgcaga aaatgatatt gccgctcgcc gctgccgcga atgtgacacg
    16981 gtactggttg atccggatga tatgttaaaa gcggcgctac gactgaaaga tgcgctggta
    17041 ttacgctgta gcggcatgtc tttgcagcat gggcacgacg agaaaggcga atggttgaaa
    17101 atcacctatt acgatgaaga cggcgcggat gtgagtgagc gtttccgtct gcaaacgccc
    17161 gcccagcgta ccgccttcga gcagcttttt atccgcccgc atacgcgcac accgggcatc
    17221 cctctgcgct ggatcaccgc cgccgatatc ctcgcccagc aagccttact gcgacatccg
    17281 gattttgtcg tcgcccgcat gaaaggtcag tactggcaag tgcgtgaaaa agtgttcgat
    17341 tacgaaggtc gttttcgtcg ggcgcacgaa ttacgcggtt aatggtagtt ttgattgatg
    17401 tataaggcga ttgagtatag aatctcgccc gcttttgcat acgcaaagca gatcacttac
    17461 ctgttgctgg gtcgcctgta gcaggattaa tttaagagag aaagaaatgt ttactatcaa
    17521 cgcagaagta cgtaaagagc agggtaaggg tgcgagccgc cgcctgcgtg ccgctaacaa
    17581 gttcccggca atcatctacg gtggcaaaga agcaccgctg gctatcgagc tggatcacga
    17641 caaagtcatg aacatgcaag ctaaagctga attctacagc gaagttctaa ccatcgttgt
    17701 tgacggtaaa gaaatcaaag ttaaagctca ggacgtacag cgtcacccgt acaaaccgaa
    17761 gctgcagcac atcgacttcg ttcgcgctta attgctgaat aagttgtaaa aaaccccgct
    17821 ccggcggggt tttttgtatc tgcagattat gcctgattac ggtattgcta ttttttgcag
    17881 gccagataag gcgttcacgc cgcatctggc attcagaaca caaaaccgat taattgccgc
    17941 cagacgtccg gcgctgcaat tggtcgcgca aattcggcgg cgtgccttta atggtcaagg
    18001 tatcggttgc tggatcccag aagatgcgtt cacctaacag catcgcatca aagttaatag
    18061 tcaagccacc gccgctaccg gcaaacttcg tcaactgacg cagtgtgcta cggtctgccg
    18121 ggaagctctc ttccagttcg tagccttttt cagccgcaaa ctccgtgaag ctcacttcgc
    18181 ttacgcctgc cagctctttc gacagcgatt ccagttcgat ctcttcgcct gcctgcagct
    18241 gctcattaca gtagctataa acctgctggc gcacgttctg tcgctctgct ttatccagtt
    18301 gtgcttcggc agtgaaatca tcaaccgctt gcaacagccc acggttttgc gctttcgcgt
    18361 tcaatccttc gctggccccg aggaaatcca taaagaaatc cgccactttg cgccctactc
    18421 gccctttcag gaaagtgaga tagcgggtgg actctggatt ggtttcccat tcggttaaat
    18481 cgatacgcgc aacaatatcc gcatgattga tatcaagata atgggttggg ttgatgtcga
    18541 gattttcgtt aacgcgcata ctgctcaggt tgctcagaac tgccaccagc aaatactcca
    18601 ccgccagata acggtagtgg caaaacagca cgaatccacc gtcagcaaaa ggatatttcg
    18661 ctaattcgtc acgcaggcga ccggttgccg cgcggctaaa tgccagaaag tcctcttcgc
    18721 cctgacgctg taagcgcagc gtctgcgcca gttcactctc ttcgctaaac aagccgtaag
    18781 ctttattttt ggcgctatag acccgatgca gttctgccac catctcaacg acggtttctg
    18841 tcggttccag caatgaatcg cgcaacacca gctcaaggtt ctgctcatca cgcttgataa
    18901 gctggtgcag agcaatctgg ttgatatcca gactcatgat aaactctcct ttaagaccgg
    18961 gcggtattca accaccgcca gttaagcgac gcaataaaag atggcggtca ttttacagaa
    19021 ggcagcgtaa cgagaatgat ttaacgcata aaaaagcaga aaaaaaaccg ttgctacggt
    19081 aatatgttgc cctttcatca actaactgat ttcgatttat gccacaaatt tcccgctaca
    19141 gtaatgaaca ggttgaacaa ttgctcgcag agctgctcaa cgtactggaa aaacataagg
    19201 ctccgaccga cctttccctg atggtgttag gcaacatggt cactaacctt atcaacacca
    19261 gcattgcccc ggcccaacgc caggcaattg ccaactcttt tgccagcgcc ttacagtcct
    19321 ctatcaacga agacaaagcg cactaaggga aacagataac aggttatggt aactcatcgt
    19381 cagcgctacc gtgaaaaagt ctcccagatg gtcagttggg ggcactggtt tgcactgttc
    19441 aatattctgc tttcgctcgt cattggcagc cgttacctgt ttatcgccga ctggccgaca
    19501 acgcttgctg gtcgcattta ttcctacgta agcattatcg gccatttcag cttcctagtg
    19561 ttcgccacct acttgctgat cctcttcccg ctgaccttta tcgtcggctc ccagaggctg
    19621 atgaggtttt tgtccgtcat tctggcgacg gcgggaatga cgctattact gatcgatagc
    19681 gaagtcttta ctcgtttcca tctccatctt aatcccatcg tctggcagtt ggttatcaac
    19741 ccagacgaaa atgagatggc gcgcgactgg cagctgatgt tcatcagcgt gccggttatt
    19801 ttattgcttg aactggtgtt tgcgacgtgg agctggcaaa agctgcgcag cctgacgcgt
    19861 cgtcgacgct tcgcgcgccc gctggccgca ttcttattta tcgcctttat cgcctcgcat
    19921 gtggtgtata tctgggccga tgccaacttc tatcgcccga tcaccatgca gcgcgctaac
    19981 ctgccgcttt cgtacccgat gacggcgcga cgtttccttg agaagcatgg cctgcttgat
    20041 gcgcaggagt atcaacgccg tcttattgag caaggtaatc cagacgccgt ttccgtacag
    20101 tatccgttaa gcgaactgcg ctatcgcgat atgggcaccg ggcagaatgt gctgttgatt
    20161 actgtcgatg gcctgaacta ctcacgcttc gagaagcaga tgcctgcgct ggcaggtttt
    20221 gctgagcaaa atatttcgtt cacgcgccat atgagctccg gcaacactac agacaacggc
    20281 atctttggcc tgttctatgg catctcgccg agctatatgg acggcattct gtcgacccgt
    20341 acgcctgcgg cattaattac tgcgcttaat cagcaaggct atcagctggg gttattctca
    20401 tcagatggct ttaccagccc gctgtatcgc caggcattgt tgtcagattt ctcgatgccg
    20461 agcgtacgca cccaatctga cgatcagacc gccacgcagt ggatcaactg gctgggccgc
    20521 tacgcacaag aagataaccg ctggttctcg tgggtttctt tcaatggcac taacattgac
    20581 gacagcaatc agcaggcatt tgcacggaaa tatagccggg cggcaggcaa tgtcgatgac
    20641 cagatcaaac gcgtgctcaa tgcactgcgt gattctggca aactggacaa tacggtggtg
    20701 attatcactg ccggtcgggg tattccactg agcgaagagg aagaaacctt tgactggtcc
    20761 cacggtcatc tgcaggtgcc attagtgatt cactggccag gcacgccggc gcagcgtatt
    20821 aatgctctga ctgatcatac cgatctgatg acgacgctga tgcaacgcct gctgcatgtc
    20881 agcacacctg ccagcgaata ttcgcaaggt caggatttgt tcaaccctca acgccgtcat
    20941 tactgggtta ctgctgcgga taacgatacg ctggcaatta ccaccccgaa aaagacgctg
    21001 gtgctgaaca ataacggtaa ataccgcact tacaacttac gtggtgaaag agtgaaagat
    21061 gaaaaaccac agttaagttt gttattgcaa gtgctgacag acgagaagcg ttttatcgct
    21121 aactgattaa ttataaatca gttagcgaaa tatcttactt gcaatcggtg tggaaaacgg
    21181 tagtattagc agccacgagt cggcacgtag cgcagcctgg tagcgcaccg tcatggggtg
    21241 tcgggggtcg gaggttcaaa tcctctcgtg ccgaccaaaa atcccaagaa aaaaccaacc
    21301 cttacggttg gtttttttat atctgcaatt aattcgataa acagaccgtg acacatcaca
    21361 gcctgtttat tttctgttat cagaacgtcc agaccacacc cgcctgagta ttccacggcg
    21421 attctacccc tgcgccattg ccatagctga ctgacaaatg accgcttaac gtcggggtaa
    21481 acgatgacct tatcccagcc tgataaacgc cactggttcc agccacatca ttattaaatt
    21541 tgccatcgtc attaactttc acctggttag aatcggcgta ttcctgacgc acggcggctt
    21601 tcagccaggg ttccagcgtc gtaccgtttt gcaggtccat gtgatagctt accgccgttc
    21661 ccgcttcagc gcgtaatatc cgggtatttc ccacatccgc gcgcatgccg tttgataacg
    21721 tgtagtcctg accatctgtg gtaaagccgg taaaggccag atagggtcta acactccaca
    21781 atccgtcaac ccaacggaac ccgctctcaa catgagcacc cgcgccgtta ctattgtaat
    21841 cgccaaacgc tgttgcccca ttactcatct tgccatggat ggtgttggca aaacggtcaa
    21901 ctttcaccac cccatcaaca taggcaccgt tctgatgctc ccaaccggca taagccccca
    21961 gggtatagct atcgatatta ccttttccgc cgcgatcaaa accaatatca gaatgagagt
    22021 aaccaaagaa caagccgcga attgtactgc tttcttcacg ggagaaacgg ctatcgatac
    22081 cgagcgtcag gcccgtcaat gtttgctcaa aaccagctcc cgcatcagtg gtcacgttgt
    22141 tgcgggtgtt aattgccgaa ctccacatca ccgtatcgta actaacgcct tttacgctac
    22201 caagacgctc acgcacggtg tccagttctg catcaaatac cagcggttgt gcggctgcca
    22261 tgttcagcac atcagtggtt gaaggggtaa tttgcgcgcg attctctgcg aggctccagc
    22321 tatggttgcc attatccagc aaggtatatt catacgtacc gatatcaaca acgcctccgg
    22381 cattgcccaa cgtaaatgca gcatcaccgc cgcccgttgt taccagtgta aggctatctc
    22441 ctgctgccgg gctggcaccg gtgtccgtca cgaatatttt gaaatcacct gttgcctgac
    22501 cggtgacgtt gagctgatcg ctctgatgat tggccatatc ggtacgcata taaaaattgc
    22561 cgtttcccga cagggtattg gtggtcaacg taatgaaatt ttccgcactt gccgtcacag
    22621 atgaacgagt cactggctca gccatcataa tcacatttgc gccggacatc gcaaggttgg
    22681 ctaagttgag gcgagtgttt tggttcgtcg tagtgatatc ggcaatgatg gtccatgcgc
    22741 tattttcgag cgaaacgtcc gctttgctgg tatccacggc accatgcgtt ctaaaagaag
    22801 cgccctcaga aaccactacc ttacccacca acatagcggg tgcataattt atttgcggct
    22861 tcatgacctc aagaatgcca tcattccctc tgactgacac gttaaccatt gtgccagccc
    22921 agacattagc gcggccattg ttgattacca tattttcaga gatggctttt gaggaatacg
    22981 tgatacttcc attctggtct gatcgaccga gatcatatac cgcatttgcc tgcactttcg
    23041 taccagtatc ctttcccagc gattgcatag tggcatgctc atcaaggata gtgtcaatgg
    23101 cctgcgtgtc ctccataaca atgagcccgg aaccatcatc cagttcataa ttttttgaca
    23161 caccatcctt tatactaaat tggcctttac tgtttgtacc actcacttcc agcgcattcg
    23221 ttgagacaat taatttcccg cctgcttttt tatcaacgcc agttgcagtc ccgccatcca
    23281 taacctccag taagccgcca ctatccacag tagtattata agcgaagtca ttttcttcaa
    23341 ctcgcaaact tccgccgttt tccaacaaca tattattagc aataccattc ttgatatcga
    23401 actgaccgag acggtttgtt ccgaatacct ccatggcccg cgtggttgtt ttaatagcac
    23461 cgcctgtttt ctgatctacc gcaagcgcaa atcccccctc tttaacctcc agtagtcctc
    23521 ccttttcgat agcggcaaaa acggcccccc catctttttc aacgattaag cgcccgcctt
    23581 taatcgtggt atcattcgct gggccataaa cctgtagcac accattattg agggtagtct
    23641 ggtttgccgc gccacccatt tgaacaattt gataaccatc atttaatact gagtattctg
    23701 cggtaccatt catttcaatg tattgatacc ctccgttaat ttcagtatta ctacttacac
    23761 caaattcttt tatatgctgt tctccgccag gattgatttt cgtattattg gcggtcccat
    23821 aaaccagctg gatatctttt tccaggataa caccatcaac tgtctcacca tactcaactg
    23881 ttgatgccaa tgctatgggt gaatataacg ctgtagccac cagaacagat attgcggaac
    23941 gacaaagaga aacagaacca gattgatgca ttgagctttc atcctatgaa attaattgca
    24001 gttaaaagca ttgggtacag aaaataccca tagctccata cccggagtca gtttttaaaa
    24061 actgtttaaa gaaatgcaca agtattgtga ttgatttttt agttgttttt cttgatgaga
    24121 agctgatgca aaattccgtc tttataataa aaatgatgcc aaagcgaacg acaaggttgt
    24181 agttttcact acatgtccat acataaaatg gggtaacatt cacgcgcctg gtagcgttac
    24241 caacgctacg ctcaaacata atgattctaa taaaacctca ggagactact atgcctgaag
    24301 caacgccttt tcaggtgatg attgtggatg atcatccact tatgcgacgc ggtgttcgtc
    24361 agttactgga gcttgatcct ggctttgaag tggtcgccga agcgggcgaa ggcgcgagcg
    24421 cgatcgatct ggcgaataga ctggatatcg acgtgatctt gctggatctc aatatgaaag
    24481 gtatgagtgg cctggatacc ctcaatgcct tgcgcaggga tggcgttacc gcgcaaatta
    24541 ttatcctgac cgtatccgat gcctccagcg atgtctttgc gctgatagac gcaggcgcag
    24601 acggttatct gttgaaagac agcgacccgg aagtattgct ggaagcgatt cgtaccggag
    24661 cgaaaggcag caaagtcttt agcgaacgcg tcaatcagta cttacgtgaa cgtgaaatgt
    24721 ttggcgcgga agaagatccc ttcagcgtgc tgacggagcg cgagctggat gttctgcacg
    24781 agctggcaca ggggctgtca aataaacaga ttgcatcggt gttgaatatt tccgagcaga
    24841 cagtaaaagt acatattcgc aatctgctgc gtaaactcaa tgtccgctca cgcgtggcgg
    24901 ccaccattct gttcctgcaa caacgcgggg cacaataaaa atagcccgat ggatttatca
    24961 tcgggctgag atttatgaca aacgcaaaac tgcctgatgc gctacgctta tcaggcctac
    25021 gtggatcgat caatttattg aatttacaca attttgtagg ccggataagg cgttcacgcc
    25081 gcatccggca taaactaagc gcactttgtc atcagtctag cccgatggca tcaccatcgg
    25141 gcctcttttt atttactctc ctgcggcgac aaatgttgca tcgcctgcgc gatactacgt
    25201 tcaatcaccg cacggcgagt atcgttggca ggtaagagtt tcaacatcat ctcccacgcg
    25261 gcaacggctt cgccaaatcg ctgctgctca aaggcattaa acgcatacat gcttagcaca
    25321 cggatattgc tatggtccgt tctcaccagc tgacgtagca gttcaccgcc gaggcggttg
    25381 tcgttgggat cagatgaacg agtcaacgct tcagcgtatc ccagtgcggc atcgctgttc
    25441 ttcggatcga ggcgatacgc agtagcgtat gcatcggtgg cgatactggc gttacccagc
    25501 gccatgccaa cgcggcccaa cataatccag ccttctatat ctcccggatt tttttgcagt
    25561 tgagtacgca tccccagcgc aaggcgcgac atctcttctt cgttgagcgg atcggctttc
    25621 gggtccagcg ccctgtccag caacgccgga gcctgtgccg tggcctgctg ccagattttc
    25681 acctgctgat aattgccagt ctggtagtag ctgacgccag ccacaattaa cgccaccaca
    25741 atacccggca gataaacaac atatccggca cgcttacctt ccggcacgct ttgttcagga
    25801 aacgcttccg gcaccacgcg tacccgacgc cgcgagcggg cgtatatgac ccaaccgcca
    25861 atgccaatag ccactactgg cagcacccac agcagcacgg tcagcggcgt taacggcgga
    25921 tcgtaagtga cgaagttgcc gtaacgcgcc accatataat cgacaatctc ttttttactt
    25981 ttaccttcct gcatcagttc atacactttc tgacgcaggt cggtggcaat catcgagttg
    26041 gaatcggcaa tgctgttgtt ctggcatttc gggcagcgca gttcttcagt gagctgacgg
    26101 aactgttgtt cctgtgcttc atctttaaac tgcaacacgt cgatggtcgc cagcgctgag
    26161 ccggagatca tcagcatcag cacgcccaat aaaaacctca ttgtgcggcc tccttactgt
    26221 atttctccca cagcggcttg atctcttctt cccagacgcg aggattgaga tcgcccgcat
    26281 ggcgatagcg aatgataccg ttgccgtcaa taaggaaggt ttctggcgcg ccatagacac
    26341 cgagatccag ccctaacatg ccgtcgccat caaacaggct tagcgcgtaa ggattgccca
    26401 gctctttcag ccagctgatt gccttctggc gatcgtcttt atagttcatg ccgaccacgc
    26461 gaatgccctg cgctgaaagc tgattcagat attgatgttc cgcacggcag gtcggacacc
    26521 aagtcgccca gacgttaagc agtactggtt tgccctgagt cagcacatcc gcctgataaa
    26581 actgccccgg attgtccagt gattcgagac gaaacttcgg cacaggcttg ccaatgagcg
    26641 ccgattccag attggtcgga tcatcccctt cggcattacg cgccagctgc cacagcagcg
    26701 ccgcggcaat cgccaggaag ataatcaacg gaattaacaa tactttgcgc ttcatacggc
    26761 ctccggcgca gttttttgcg gactcacgcg cttacgatag cgaggatcaa acagacacag
    26821 cagtccgccc aacgccatca tcagcccgcc cgcccaaatc cagcgaacaa atggtttgta
    26881 gtaaagacgc acggcccacg cgccgttttc cagctcttca ccgagcgcgg cgtacaggtc
    26941 acgcgtgatg ccgccgtcaa ttgccgcttc ggtcatcatc gacccggcag tgttgtaata
    27001 acgtttttcc gcatacagca ccgtttccgg tttaccctct cgcgttacgc caatagtcgc
    27061 cacaccgccg cgccagttcg ggccagtcac ctctttgaca tcacggaagg tgaagcgata
    27121 ttcatgaata tcgacgctat cgccggactt catgcgcaca tcacgctcaa cgctataatt
    27181 ctggctaaag gcaatgccaa caattgtcac tgccagcccc aggtgagccg ccaccatccc
    27241 ccaataactg aaggtggttt tcgtgccgcg tgaaatacgc agcgcagctt ccgcagttgc
    27301 cagcaccgca atccagcagg ccattgccag gccgagcacc gtcatcgcca caactttgct
    27361 ttcgaacagc cacggcaaca gcagagacag caccagcgta gagatgaagg cgataatcaa
    27421 taaattgcgg attttacgtg ggcgatcgcg cccccagcgc accagaggac cgacaccaag
    27481 cagtagcgca aacggcacca tcagccaggt aaacatggtg ttgaagaacg gttcgccaat
    27541 cgaaatactg cccagtccaa gttgcttgtg caccagcggc agcagcgtcc ccagcaacac
    27601 caccagcatc gcggcgacca gcaaaacgtt gttcgctaac agcaaagatt cccgcgacca
    27661 cagcgcattg tttacgcgtg agcgaacttt gtgtccacgc gcggcaaaca gcagcagcga
    27721 accgccaatc accagcacca taaaggcgag gataaacata ccgcgcgccg gatcagacgc
    27781 aaacgcgtgt accgatacca gcacgccgga acgcacgagg aaagtcccca acagacacaa
    27841 cgagaaggca ctgattgcca gcagtaatgt ccacgctttg aagctggcgc gttgttcagt
    27901 gaccgccagt gagtgcatca gtgcagtccc caccagccac ggcataaacg aggcgttttc
    27961 taccggatcc cagaaccacc agccacccca gccgagttcg taataggccc atgcggaacc
    28021 gagcacaatg ccgagcgtta ggaaaatcca cgccgccagc gtccacgggc gagtaaaacg
    28081 cgcataagtg ctgtccagac gcccgctcag caaagaagca atggcaaaag caaacgccac
    28141 cgagaaaccc acgtacccca tataaagcag aggcggatgg aagatcagcc ccggatcctg
    28201 taacagcggg ttaagatcgc gcccttcaat cgggaagttc ggcagcgtgc gtgagaacgg
    28261 gttagaggta aagagaatga acagcaaaaa gccgacgctg accatcccca ttatcgccag
    28321 tacacgggcc acaatatcca gcggaatacg ctgactaaaa atcgccaccg caaaggtcca
    28381 gccgctcatc agcagcaccc acagcagaag cgaaccttca tgcgcgcccc aagtagccgc
    28441 cacgcgatac cacaccggaa gctgggtatt ggagttgctg gcaacatagg tgacggtaaa
    28501 gtcgttgacc acgaaagcat tgaccagcac cagaaatgcg ccagccacag acataaacag
    28561 cagccaggca aacaagcggg aagacgccat catgcgcgca tctccgcgcg ccacgcccca
    28621 tagcggatac acggacagca gcagcgcaat ccctaacgcc aagcacagca gcccgtttcc
    28681 aatttccggc atcatgatgc tgggtcctta taaacactcg ccgggcgacg gtggttagct
    28741 tccatcgctt tctcaacttc tggcggcgtg tagttttcgt cgtgtttcgc cagcacttct
    28801 ttcgcgagga tatgattgcc tttttccagc tcgccctgca ccacaacgcc ctgcccttca
    28861 cggaacagat ccggcaaaat gccttcgtaa gagacatcca ctgagccttc agcatcgtaa
    28921 atggtgaagg tcactttcag cgaattggga tcgcgctgca cgctacccgg catcaccatc
    28981 ccgccaacgc gcagacgctg accgacttcc ggcatctgct gagtttcacg cttgccgtag
    29041 agaatttccc ccggcgtata aaagagatcg atattcgagc gcagcgcata tagcaccaga
    29101 ccgatagtca gcgctagccc tgccaacacg gcacaggcaa tccacaagcg gtttttacgg
    29161 cgaatattca tgcagcctcc tgctgttgcg cagcacgtaa acgcgcctca cgcgcccgct
    29221 gttgcgccac accacgcaga attgcgcgat gttgcatcac cgagtgcacg accaaaacca
    29281 ccagcggaat aacggtcatc accaccgcca gccagacaaa aaaggcgtaa ccgcccattg
    29341 cgaaaaattc attccaggaa gcaaatgcag gggtcattta cggcctcttt tcagtatcag
    29401 ttcactcacc cacggacggc gtttttccat cagcaaaatc aaattacgca tccgcatcag
    29461 cgtcagcgtg gcagacagga gcaggaagcc aaaaatcgac cagcgcagcg gcgaacgcat
    29521 cgccggatcg atactttgct gcatccgcgt tgatccctga tgcagggtgt tccaccactc
    29581 cacggagtaa tgaataatcg gcagattcac cacgccaatc agcaccatga tacctgccgc
    29641 acggcccgcc agacggcggt cgtcgaaggc gtgccacagg gcaatcacac ccacatacaa
    29701 aaacagcagc accagttcag aagtcagacg tgcatcccat acccaccagg tgccccacat
    29761 cggttttccc catgcagagc cggtaaccag ggcaataaag gtaaacacgg caccaatggg
    29821 ggccatcgcc gccaccgcca ggttggccat tttcatctgc cagacaaggc caataaacgc
    29881 cgccaccgcc attgatgcat aaatgcccat cgaccagatc gccgcaggca catgcaggta
    29941 gataatgcgg tagctatttc cctgctgata atcagccgga gcaaagccga atccccagat
    30001 ccagccgacg gtaagcacga ccacactggc aattgccagc cacggtataa accagccaca
    30061 gatttgatac agccgtggtg ggatcgccag ttgatgcagt gttttccaca tagtttcgat
    30121 accagactcg aacaaaaatc agtaatccag cgttattgaa tgctgattcg taacgctgcc
    30181 gccgtcgcaa aaggacttaa tgtcgcggtg cctgccagca acgcgcctaa aattgccaga
    30241 tacccatcaa cgggcaaatg catagaagcc gcgtccatcg cggcggtggc aaagatgagt
    30301 aatgggatag tcagcggtaa caccagtatg ctgagcagca caccaccgcg cttaagtccc
    30361 actgtcagcg ccacgcccgg tgcaccgaga aagccaagcg taggcgttcc cagcagcagc
    30421 gtcagcgcca tcacttgcca gccataaaca tccattccca gtagcattgc taccagtggc
    30481 gaaaggatga gtaacggcag accggttacc atccagtgcg ccatcacctt cgccagcaca
    30541 acggcgggca agggtaacgg caacaacatc aattgttcaa gactgccgtc ctgcaaatcg
    30601 tcacggaaca gtcgttccag cgccagcaag gatgaaagca gcgcagcaac ccagataatg
    30661 cccggtgcaa tacgcgccag cagttgcggc tccggaccga tactgagcgg aaaaagggta
    30721 attacaatca ggaagaacca cagcgggttg gcgatttcgg cgctatggcg aaacgctacg
    30781 cgcagttcaa gacggaaaat acgccagaac atcatgcggc cctcgtttgc gtcagtgaaa
    30841 tgcggcgaat tttactttca gcaacgttga gcggctggtg ggtagtcaga atcacaatcc
    30901 ccccctgctc cgtatgctgc gccatacgct gggtcagacg atcgacgccg ttaacgtcaa
    30961 tcgcggtaaa aggctcgtcg aggatccata atgtggcacg ggtcaaccac agacgcgcta
    31021 aagcgacgcg gcgttgttgc ccggcagaaa gttgatttac cggaatatct tcgaatccgg
    31081 caagcccggc ctgcgccaag gcttccagac attgtgcggc atcgccatcg cgatgataaa
    31141 agtgcagatt ttctaacgcc gtcagccggg ttttgatccc cggctgatgg cctatccata
    31201 acagggtttg atggtagctg tcgcgtacct gatgcaaggg ctgcccttgc cagagaacct
    31261 cgccagcgtc agggcgagac aaccccgtca gcaaacggag aagcgttgtc ttccccgcgc
    31321 cgttgctacc ggtgatttgt acccactctc ctgcgttcag cgtaaatgac aagccactaa
    31381 ataaggttcg ttcatcccgc tcacaaagta actctctggc ttcaagcata cccacgcaat
    31441 aaccctgtta aaaacctggc tcgacttcac gcatatcggg cagcttgtgc gcgatccctt
    31501 tatggcaatc aatacaggtt tgcccatctt tcaccgcctg gtcatgcatc ttcgcggcaa
    31561 ccgatttctg ggcggttgta tccatatact cgaagttgtg acagttacgg cactcctgcg
    31621 agttattgtc cttcatgcgc cgccactcat tctgtgccat cgtcagacga tgagcttcaa
    31681 atttctgcgg cgtgtcaata acgccaaaaa ttttaccata cagctcttta cttgctttga
    31741 gcttgcgtat catcttcggc acaaactcgt gcggaacgtg acaatccgga caggtcgcac
    31801 ggacgccgct acggttgttg tagtgcacgg aatccatgta ttcctgatac accgtgttgc
    31861 gcatttcgtg gcagctaatg cagaactctt cggtattggc tttttccatc ccggtgttaa
    31921 agccccccca gaagacgatg ccgccaacaa aaccgatcaa cagcagcgtc cccagcgcca
    31981 gatggctggg ggtacgccac catttccaca ggcgcttaat cagaccaggc ttacggtcag
    32041 aatttcccat aataacctct tatttcccgt aaccttttga tggggtaaag gtattcccca
    32101 cgattggcgc ggtatcggcc tgcggtacgt gacattgcag acagaaataa cgacgcggag
    32161 ccacttccgc gcccactttg ccgtcgctgt ccataaagtg agtaggactg atacgcggcg
    32221 cgccagtggt gcgatagctt tcgacaccgt ggcactgcaa gcagcgattg gtattggtcg
    32281 ttacctgata accttcaacg ctatgcggga tcattggcgg ctgattcaca tagttcagcg
    32341 gcatccgatc ctgctctttt ggcatccgaa tggccccttc ctgcgtcccg gatacttccg
    32401 gcgactggct aaaatcgacg ccattagccg cccaaaccgc gccgcttacc accagggcca
    32461 gcatcgccgt ccattgacac agcgctttct tcaggtcatg gcttttcatg atttcgctcc
    32521 cgaactccat cgtgtagtta ttgtaaatac atcctcagaa cagacatcca cgcagcgacc
    32581 gcacgtcatg caatcgcggc tggtgacctg caccgggctt tgttcatcca gcaccggggc
    32641 acgtagcaca tgcggttccg ggcaaacatg aaaacaatcc atacagcggt tacatttctg
    32701 ccgatcgctg gccgcaacgg taatcacacc tttgctaccc agcacgccat acagcgcgcc
    32761 aaccggacaa atgtgcccgc accagccgtg ttcaacgacc agtagatcaa ataaaaacag
    32821 cgcgagaata agcagcgcgc cgctgccgaa gcccattacc aggctacgcc ccatcagaga
    32881 aacggggttg atccattccc aaatgagcgt gccagttaag gctgatccca ccagaatgac
    32941 caccaacagc acgtagcgaa tgtggcgagg gatcgtcgca gactgattga ggtcaaagcg
    33001 cctgcgtaac cagttcgcta agtcggtaat cgggttcagc gggcagaccc agctgcaaaa
    33061 taatcgcttt cccgccaggg cgtagagcac ggtgataatc accgcaccgg tcaacgccac
    33121 cgtggcaggc agatgaccac tggcaagact ttgcagcgtc atcagcggat cggtcagcgg
    33181 aacggtgtcg aataacaggc tactgctgta gttgccgtgc aagatccaca caccaaacca
    33241 tggaccgctc aaaaacatcc ccagcacgaa gaactgacaa aggcgacgta acaccagcca
    33301 acggtgactg cgccaccagc cttttttctc cagcgcctcg cgcccggcgt cacgtttacg
    33361 atttgccatt gttcccctcc agccagccga agcggtaatg gtgacctaac tcccctttcg
    33421 ccagtgacag cggtaacacc ttgattgccg gttgttccag cacgcatact ttttcgcact
    33481 taccgcaacc ggtacaggcg tcgctgtgaa ccgtcggcag aaagcgggcg tgcttaccgg
    33541 tacgcgtgtt gcgctccagc tccaaggtga tggcctcatc aattttcggg cattcgcgat
    33601 aacaaacatc gcagcgcagc ccctgaaagt tgagacagtt ttcctggtcc accagtaccg
    33661 ccagccccat ccgcgcgtcg tcgatcgatt caatctcacg atccagcgca ccgcttgggc
    33721 acactttggc gcacggaatg tcctcacaca tttcgcaagg aatatcccgt gcgacaaaat
    33781 atggcgtgcc cgccgacaga ccagaggcca gcgtcgccag ttttaaggtg tcgtaagggc
    33841 aagcctgaac acactgaccg caacgcacac aggcactggc aaaggcgttc tcgtttatgg
    33901 ctccgggcgg gcgcaaccgc acgccagatg cgcgtgcggt ttgctgttgt aaccccagcg
    33961 ccacacccac ggcagccagc ccgcctgctg tgcgaacgac atcgcgcaga aagcggcggc
    34021 gaccattttg aggtttcgct gaccgggaca taatggctta caccttctcc agtttgaccg
    34081 cgcacttctt gaagtccgtc tctttcgaga gcggatcggt cgcatccagc gtcagtttgt
    34141 taaccagctg tgcggcgtcg aagaacggca tgtacaccag gccctgtggc ggacggttac
    34201 gaccgcgcgt ttcaacaatc gagatcactt cgccacggcg agaaaccact ttcactttgt
    34261 cgccacggcg cagatcgcgc gctttcgcat ccagcgggtg aataaacaag accgcttccg
    34321 ggaaggcgcg gtgcagttcc ggcacacggc gagtcatact gccggtgtgc cagtgctcca
    34381 gaacgcgtcc ggtagagagc cacaggtcgt actcttcatc cggtgcttcc gccgccggtt
    34441 cgaacggcag tgcgaagatc accgctttgc catccggttt accgtagaac ttatagcctt
    34501 cgcccgcttt cacgtacggg tcgttacctt cgctgtaacg ccactgtgtt tctttaccat
    34561 tcaccaccgg ccagcgcaga ccgcgcgctt tgtggtagtc atcgaacggt gccagatcgt
    34621 gaccgtgacc gcgaccaaac catgcgtact cttcgaacag ccctttttgc agatagaagc
    34681 ccagctcgcg ggattcatcg ttcagctgat cttccgccag ttcggatacc gggaatttgc
    34741 tcacttcggg ggtggcatac agaacttcgt acagcgtttt gccacgcagt tccggtttct
    34801 tcgccagcag ctcttccggc catacttctt cagttttgaa gcggcgggag aactggacta
    34861 actgccagag atccgatttc gcttcgcctg gcgcctgtac ctgctgacgc cagaactgag
    34921 tacggcgttc ggcgttaccg taagcgccct ctttctctac ccacattgcg gtcggcagga
    34981 tcaagtcggc ggccagcgca ctgactgtcg gatacggatc ggagacgatg atgaagttgc
    35041 gcggatcgcg ccagcccggc atacgctctt cattgatgtt cggcccggcc tgcatgttgt
    35101 tggtacacat ggtccagtaa acattcagct tgccgtcttt cagcgcacgg tcttgtgcca
    35161 ccgcatgaag accgattttc gccggaatgg tgccgctcgg gatattccac ttcttctcac
    35221 agatatcacg atgtttctcg ttagttacca ccatgtccgc aggcagacgg tgagcaaagg
    35281 tgcccacttc acgcgcagta ccacacgcag aaggttgccc ggtcagggag aacggaccgc
    35341 aacccggctg ggaaattttg ccggtcagca ggtgcaggtt gtagaccagg ttgttagccc
    35401 acacaccacg ggtatgctgg ttgaagccca tcgtccagta ggagatgact ttcttgttcg
    35461 gatcggcata cagctgcgcc agttgttcta actggtcttt cggcacgccg gtcatttcag
    35521 cggttttttc cagcgtatac tcagcaacga aggctttgta atcttcaaag ctcatcggtt
    35581 cggaggcgtc agaacccgga ttcttcgctg ctttttccag cggatgggtc ggacgtaaac
    35641 cgtagccgat gtccgtcgcc cctttgcgca ggttaacgtg cttgctgaag aagtcctgat
    35701 ttatcgcatt gttttgaatg atatagttgg cgatgtagtt caggatcacc aggtcagatt
    35761 gcggcgtaaa gatgatgccg ttatccgcca gctcgaagct acgatgctgg taggtagaaa
    35821 gcaccgccac ggtgacgttc tggttagaga gacgactgtt agtgatgcgt gaccagagga
    35881 tcgggtgcat ctccgccatg ttagcgcccc acagcacaaa cgcgtcagcc tgctcgatgt
    35941 cgtcatagca gcccatcggc tcatccatac caaaggtacg cataaagcca actactgccg
    36001 acgccataca gtgacgtgcg ttcgggtcga tgttgttcga acggaagccc gctttgaaca
    36061 gcttggacgc ggcataacct tcccagatag tccactgacc agaaccgaac ataccgatcg
    36121 attccggccc tttttctttc agggcggttt tgaatttctc ttccatcaca tcgaaggcct
    36181 gatcccaggt gattggggta aattcgcctt ctttgtcata tttaccgttt ttcatacgca
    36241 gcagcggctg cgtcaaacgg tctttaccgt acatgatctt gggcaggaaa tagcccttaa
    36301 tgcagttcag gccacggtta accggtgcgt ccgggtcgcc ctgacaggcc accacacgcc
    36361 cctgctgcgt tccgaccaga acgccgcaac cagtaccgca gaaacggcac ggcgctttat
    36421 cccatttaat ggcttcctgc tgaccaacaa cggcgcgggc aacgcccggc acgctgagac
    36481 cggcagccgc cgcagcggcc gcaacggcgt tagctttcat aaagctacga cgactgagtt
    36541 tcatggtgtt tcctcacctt gctcttcctg ctggtgataa accagcgaca ccgccagcac
    36601 gccctctacg ttgcgtactg actcaatggt ttggatcagc gtttcgctgt cttctgcttc
    36661 caccaccaca atcaactgac cgctcggcgc gtcgctgaca gcaacttcac agccgggaaa
    36721 ggcgttcagt tgggtgctga tgtctgaaat tcgttcgctt ttggcctgca cgaccaggct
    36781 gcaaacttgc cagttagtgt gcatggagat actccgcagt tatggctgat accggacagc
    36841 tggctgcaca tgctccacat ccgttgcaaa gttggctatt aagttgcgcc tggtagatcc
    36901 cggagagcgt cgggcgaaag ataattgcca ttggctcaca gctatcctga cagcggcggc
    36961 attcaactga ctgatacgcc aggcacgcgt ccccgatggt gaactgcaaa tcccaggccc
    37021 tggtgtggcg cggagaaaat agcgattcgg ggcaagcctg cgcgcaggcg taacagaagc
    37081 tgcactcgtt atttttgaaa ttaacgctcg gatagccacc cgtgccgcgt tgcagaatgt
    37141 tgttttcaca agcattaata caggcgtcgc agcgggtgca atgggtcaga aaatgagatt
    37201 catcaccgga ccagggcgga cggataccgt tactggcttt gcgccagcaa ccagtgagta
    37261 tgccccgacg ggatgcatca atcttcacat tgaccttcca tcattaacgc gctctgaaaa
    37321 ttgagagcga ccaaatagac cgcataatta ataagccatt tttatagccg ctaagatatt
    37381 aaaggatgtg tcaaagatgc ataccccgat cggggtaaaa cctgtagcag gatcaaaaga
    37441 gtggcgggaa gcgtggcaaa aacgggcttt tgctc
//
LOCUS       DAZPCS010000025        36587 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZPCS010000025 DAZPCS010000000
VERSION     DAZPCS010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36587
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.025"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..190
                     /locus_tag="ACHUQ7_001231"
     CDS             <1..190
                     /locus_tag="ACHUQ7_001231"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1134731.1"
                     /translation="PPHVKALPDTITQTSIHRTLQFIEFARSCRPDKAFTPHPAINRR
                     RLPNGRFFTYSPPSNVPA"
     gene            complement(156..1391)
                     /locus_tag="ACHUQ7_001232"
     CDS             complement(156..1391)
                     /locus_tag="ACHUQ7_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFR1134732.1"
                     /translation="MLQQVPTRAFHVIAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAVRDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(1538..1614)
                     /locus_tag="ACHUQ7_001233"
     tRNA            complement(1538..1614)
                     /locus_tag="ACHUQ7_001233"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1578..1580),aa:Pro,seq:tgg)
     gene            complement(1657..1743)
                     /locus_tag="ACHUQ7_001234"
     tRNA            complement(1657..1743)
                     /locus_tag="ACHUQ7_001234"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1707..1709),aa:Leu,seq:cag)
     gene            complement(1764..1839)
                     /locus_tag="ACHUQ7_001235"
     tRNA            complement(1764..1839)
                     /locus_tag="ACHUQ7_001235"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1804..1806),aa:His,seq:gtg)
     gene            complement(1898..1974)
                     /locus_tag="ACHUQ7_001236"
     tRNA            complement(1898..1974)
                     /locus_tag="ACHUQ7_001236"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1938..1940),aa:Arg,seq:ccg)
     gene            complement(2077..3462)
                     /locus_tag="ACHUQ7_001237"
     CDS             complement(2077..3462)
                     /locus_tag="ACHUQ7_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR1134733.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWSPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3653..4393)
                     /gene="wecG"
                     /locus_tag="ACHUQ7_001238"
     CDS             complement(3653..4393)
                     /gene="wecG"
                     /locus_tag="ACHUQ7_001238"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFR1134734.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISIVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4396..5748)
                     /gene="wzyE"
                     /locus_tag="ACHUQ7_001239"
     CDS             complement(4396..5748)
                     /gene="wzyE"
                     /locus_tag="ACHUQ7_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFR1134735.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQVLLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(5745..6824)
                     /gene="rffT"
                     /locus_tag="ACHUQ7_001240"
                     /gene_synonym="wecF"
     CDS             complement(5745..6824)
                     /gene="rffT"
                     /locus_tag="ACHUQ7_001240"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFR1134736.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(6821..8071)
                     /gene="wzxE"
                     /locus_tag="ACHUQ7_001241"
     CDS             complement(6821..8071)
                     /gene="wzxE"
                     /locus_tag="ACHUQ7_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFR1134737.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8073..9203)
                     /gene="rffA"
                     /locus_tag="ACHUQ7_001242"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8073..9203)
                     /gene="rffA"
                     /locus_tag="ACHUQ7_001242"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFR1134738.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDTLAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9208..9882)
                     /gene="rffC"
                     /locus_tag="ACHUQ7_001243"
                     /gene_synonym="wecD"
     CDS             complement(9208..9882)
                     /gene="rffC"
                     /locus_tag="ACHUQ7_001243"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFR1134739.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(9860..10741)
                     /gene="rfbA"
                     /locus_tag="ACHUQ7_001244"
     CDS             complement(9860..10741)
                     /gene="rfbA"
                     /locus_tag="ACHUQ7_001244"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFR1134740.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(10760..11827)
                     /gene="rffG"
                     /locus_tag="ACHUQ7_001245"
     CDS             complement(10760..11827)
                     /gene="rffG"
                     /locus_tag="ACHUQ7_001245"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFR1134741.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAYYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(11827..13089)
                     /gene="wecC"
                     /locus_tag="ACHUQ7_001246"
     CDS             complement(11827..13089)
                     /gene="wecC"
                     /locus_tag="ACHUQ7_001246"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFR1134742.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13086..14216)
                     /gene="wecB"
                     /locus_tag="ACHUQ7_001247"
     CDS             complement(13086..14216)
                     /gene="wecB"
                     /locus_tag="ACHUQ7_001247"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFR1134743.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGALYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIAPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14272..15318)
                     /gene="wzzE"
                     /locus_tag="ACHUQ7_001248"
     CDS             complement(14272..15318)
                     /gene="wzzE"
                     /locus_tag="ACHUQ7_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFR1134744.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15330..16433)
                     /gene="wecA"
                     /locus_tag="ACHUQ7_001249"
     CDS             complement(15330..16433)
                     /gene="wecA"
                     /locus_tag="ACHUQ7_001249"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFR1134745.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16673..17932)
                     /gene="rho"
                     /locus_tag="ACHUQ7_001250"
     CDS             complement(16673..17932)
                     /gene="rho"
                     /locus_tag="ACHUQ7_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFR1134746.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18017..18118)
                     /gene="rhoL"
                     /locus_tag="ACHUQ7_001251"
     CDS             complement(18017..18118)
                     /gene="rhoL"
                     /locus_tag="ACHUQ7_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFR1134747.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18259..18588)
                     /gene="trxA"
                     /locus_tag="ACHUQ7_001252"
     CDS             complement(18259..18588)
                     /gene="trxA"
                     /locus_tag="ACHUQ7_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFR1134748.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            18719..19984
                     /gene="rhlB"
                     /locus_tag="ACHUQ7_001253"
     CDS             18719..19984
                     /gene="rhlB"
                     /locus_tag="ACHUQ7_001253"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFR1134749.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20118..21602
                     /gene="gppA"
                     /locus_tag="ACHUQ7_001254"
     CDS             20118..21602
                     /gene="gppA"
                     /locus_tag="ACHUQ7_001254"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFR1134750.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(21649..23670)
                     /gene="rep"
                     /locus_tag="ACHUQ7_001255"
     CDS             complement(21649..23670)
                     /gene="rep"
                     /locus_tag="ACHUQ7_001255"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFR1134751.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEAHGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNYQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSTKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            23757..24038
                     /gene="ppiC"
                     /locus_tag="ACHUQ7_001256"
     CDS             23757..24038
                     /gene="ppiC"
                     /locus_tag="ACHUQ7_001256"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFR1134752.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(24084..25559)
                     /gene="ilvC"
                     /locus_tag="ACHUQ7_001257"
     CDS             complement(24084..25559)
                     /gene="ilvC"
                     /locus_tag="ACHUQ7_001257"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFR1134753.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIVRKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            25709..26599
                     /gene="ilvY"
                     /locus_tag="ACHUQ7_001258"
     CDS             25709..26599
                     /gene="ilvY"
                     /locus_tag="ACHUQ7_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFR1134754.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPLLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMANQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(26596..28140)
                     /gene="ilvA"
                     /locus_tag="ACHUQ7_001259"
     CDS             complement(26596..28140)
                     /gene="ilvA"
                     /locus_tag="ACHUQ7_001259"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFR1134755.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(28143..29993)
                     /gene="ilvD"
                     /locus_tag="ACHUQ7_001260"
     CDS             complement(28143..29993)
                     /gene="ilvD"
                     /locus_tag="ACHUQ7_001260"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFR1134756.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALCAYASLATSADKGAVRDKSKLGG"
     gene            complement(30058..30987)
                     /gene="ilvE"
                     /locus_tag="ACHUQ7_001261"
     CDS             complement(30058..30987)
                     /gene="ilvE"
                     /locus_tag="ACHUQ7_001261"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFR1134757.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(31007..31270)
                     /gene="ilvM"
                     /locus_tag="ACHUQ7_001262"
     CDS             complement(31007..31270)
                     /gene="ilvM"
                     /locus_tag="ACHUQ7_001262"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFR1134758.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(31267..32913)
                     /gene="ilvG"
                     /locus_tag="ACHUQ7_001263"
     CDS             complement(31267..32913)
                     /gene="ilvG"
                     /locus_tag="ACHUQ7_001263"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFR1134759.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(32916..32966)
                     /gene="ilvX"
                     /locus_tag="ACHUQ7_001264"
     CDS             complement(32916..32966)
                     /gene="ilvX"
                     /locus_tag="ACHUQ7_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFR1134760.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(33052..33150)
                     /gene="ilvL"
                     /locus_tag="ACHUQ7_001265"
     CDS             complement(33052..33150)
                     /gene="ilvL"
                     /locus_tag="ACHUQ7_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFR1134761.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            33502..34981
                     /locus_tag="ACHUQ7_001266"
                     /pseudo
     CDS             33502..34981
                     /locus_tag="ACHUQ7_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
     gene            complement(35006..35344)
                     /gene="maoP"
                     /locus_tag="ACHUQ7_001267"
     CDS             complement(35006..35344)
                     /gene="maoP"
                     /locus_tag="ACHUQ7_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFR1134762.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            35463..36302
                     /gene="hdfR"
                     /locus_tag="ACHUQ7_001268"
     CDS             35463..36302
                     /gene="hdfR"
                     /locus_tag="ACHUQ7_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFR1134763.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     DVY"
     gene            complement(36398..36473)
                     /locus_tag="ACHUQ7_001269"
     tRNA            complement(36398..36473)
                     /locus_tag="ACHUQ7_001269"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36438..36440),aa:Trp,seq:cca)
     gene            complement(36482..36558)
                     /locus_tag="ACHUQ7_001270"
     tRNA            complement(36482..36558)
                     /locus_tag="ACHUQ7_001270"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36522..36524),aa:Asp,seq:gtc)
BASE COUNT         8939 a        10012 c         9217 g         8419 t
ORIGIN      
        1 gccaccgcac gtaaaggctt tgccggatac gatcacccaa accagtatcc acagaacgct
       61 gcaatttatt gaatttgcac gatcatgtag gccggataag gcgtttacgc cgcatccggc
      121 aatcaaccgc aggcggcttc caaatggccg cttttttact tattcaccac catcaaatgt
      181 gcctgcataa tgtcactggc cgggcgaccg tgcgccagca aatcagccat tgctttaaga
      241 tatggcggta gatggcggaa ataacgctga tacccggcac acaaataatt cagccccggt
      301 ttgccgctgg catcgagcat gaagcggtgt ttcgggcagc ctccccaaca tgcttttaac
      361 acgttacaac tgcgacactg cgccggtaat tgcttaaatt tatcttcacc aaacacctgc
      421 tgttgcgggg aatcgatcat ttctgcaatt gtttgctgat gcatattccc cagccgatat
      481 tgcggataaa catagtgatc gcaggcgtaa acgtcgccgt tgtgctcaac aatcaccgag
      541 cgcccacagg ttggctgatg atggcaaacc gcacccggcg caccgacaaa attggcaaac
      601 gcccattcga tattcatcac gaaaatcttg ccgacgtcgc gtttgatcca gtggtcaaaa
      661 atcgccacca gaaattcacc aaattcatcg gggcgcacag accattccgt cagttccccc
      721 tgaatatcac caggcgcatg tagtttcagt ccatcgcgga cagctgtttc atcagccagg
      781 cgctcgacca ccggaataaa ctggatgaat tcgactcccg catcgcacaa aaaatcataa
      841 acctgtaacg gttgctgcgc gctggtgcgg ttgacgcaga ccagcacgtt atagtcgaca
      901 tgatgttttt gcaggagcgt cagggcacgc atcaccagtt tatgcgtggg tctgccgcct
      961 ttggtcacgc gatattgatt gtggatctca gccggaccat ccagcgataa cccaacaaga
     1021 aaattatttt ctgccagaaa tgcacaccat tcgtcatcga gcagcacgcc gttagtctgg
     1081 aaactgttac ttatcttcct gccagcacca tatttcgcct gtagcgccac ggcacggcgg
     1141 taaaactcca gcccgagtag cgttggttcg ccgccctgcc aggtaaaagc cacttcgttt
     1201 tgcagttcgc tggcggcgat atagtgacgg atatacgctt ccagcgtgtc atcgtccata
     1261 tgcgtgactg gcttttcgcg gtaaagggat tgtttttcga gataaaaaca gtagtcacag
     1321 ttcagattac aatcggaacc gctcggtttc gctatcacat gaaaagcacg cgttggaacc
     1381 tgttgcagca tggtcgctcc ttgcctgaag tcatccttca atacgttaag ggcgaaatca
     1441 taccgtgggg caatgagtta ttacgggcgt ttattgcgaa gggagggtga cgaaatgcac
     1501 agaaaacaaa aaaccccgcc gaagcggggt tcaaaattgg tcggcgagag aggattcgaa
     1561 cctccgaccc actggtccca aaccagttgc gctaccaagc tgcgctactc gccgaaatac
     1621 tgctttttga atttttagtt caattcttta aagtcgtggt gcgagggggg ggacttgaac
     1681 ccccacgtcc gtaaggacac taacacctga agctagcgcg tctaccaatt ccgccacctt
     1741 cgcattgtca caacttctaa taatggggtg gctaatggga ttcgaaccca cgacaactgg
     1801 aatcacaatc cagggctcta ccaactgagc tatagccacc actacaaatc ttgttacgcg
     1861 gtattactac caccgcagct caagcgctgg gactaaatgg cgcgcccgac aggattcgaa
     1921 cctgagacct ctgcctccgg agggcagcgc tctatccagc tgagctacgg gcgcttagcg
     1981 ccgttgcggg ggtggataat acggacttcc cacccctctg tctagtccct ttttaaataa
     2041 attgctcgtt tggttacggt ttgtgcgttt tgctgcttat tcctccagtt tatgcgcttt
     2101 accgtggcga ttaagcccaa aaactttata aatcgccgtc accgccagca taaagatgat
     2161 gccgacaaac agcgacatac gcgtatcttc attaaagtac atgccgatca aaacgcaaat
     2221 caggaatgcc attgttacgt agttggccca cgggaacagg attgagcgga acggatggct
     2281 ggcaatcgcc gctttatgtg cacgccgaaa acgcagctgg cttatcaata tcacaaacca
     2341 tggcaccatc cccggaagca cgctggcact gtagacgtag acaaacacac gctgcggatt
     2401 gggaatgatg tagttcaggc atgagccaat tagcagaata gcaatagata ctgccacacc
     2461 cgcaaccggt acgccgtgac gggaaacttt tgccattgcc gccggtaact gacggttttt
     2521 cgccagtgcg tagagcatac gtccgcaact gtacatgccg ctgttacagc cagagagcgc
     2581 agccgtcagc accacaaagt tgataatgcc cgccgctgcg gtaataccga ttttggcaaa
     2641 agtcagtacg aacgggctgc cgttgctgcc aatttcattc cacgggaaga tggtgacgat
     2701 aacgaaaatc gcgcctacgt agaaaatcag gatccgccac agcactttgc ctacggcact
     2761 gcgcagcgtc acctgcggat tcttcgcttc accggcagta atgccaatca gctccacgcc
     2821 ctggtaggac gccaccacaa tacacagagc ggtcaggaac cctttccagc cacccgcaaa
     2881 gaaaccgcca tgctctgtga gattgctaaa accaatcgac tgcccgccat tgccaaagcc
     2941 aaagaaaatc acgcccaggc caatgacaat catcacgata atcgtggtga ctttgatcat
     3001 cgcgaaccag aactcgattt cgccgtacaa ccgtaccgcc gccagattcg ccaacgccac
     3061 cagcgccacc gcgatcaatg cgggtatcca ctgcgccatc tccggggacc aaaactggac
     3121 ataaacgcca atggcggtga tttctgagat ccccaccgcc atccacataa accagtaaga
     3181 ccaggcggtg agatagccaa aaaacgggct catataacga tgcgcataaa cggcgaacga
     3241 accggtaacc ggttcgagga acaacatttc gcccattgaa cgcatgatga aaaaaacgaa
     3301 cagcccggcg atgatatagg ccaacaatac ggatggcccg gcccatttca gggtactggc
     3361 ggcccccata aacaggccga cgccaatggt gccccccagg gcgatgagtt cgatatgtcg
     3421 agcttccagc ccacgctgta gctctggttt gttatctgcc ataaatcctc gtgttgtgtt
     3481 tgcatgcttt ccggtgttac cggttatcgt tatgggtaca tcgagtgttg cgaatgtttt
     3541 cgtaattcag gagaaatggc aaataaagca ttaaaaattt gaatgctttg tgtaataaaa
     3601 aagcagacag gcgacgaagt gaccactccg tcgctttaca aagagaggaa aatcagaggt
     3661 tgccggtgta gtgccagcgt aaataacgca gcaaacgaag ctgacgctta atgcggctcg
     3721 gctgcgaaag caggcggtag agccactcca gtcccagcgt ttgccagatt ttcggtgcgc
     3781 gttttacgtg accggtgaaa acatcgtaag tcccgccaac gcccatatac agcgcatctg
     3841 gatgcaccag acggcagtcg cgcatgaaga tctcctgctt tggcgatccc atcgcaacgg
     3901 tgacgatttg cgctccgctg gcatgaatgc gttcaaacag cgcctgacgc tgctcgggtt
     3961 taaaataacc atcctgactg ccaacgatat tcacattcca ttggttgcgc agtttagctt
     4021 cagtttgcgc cagcacttca ggtttaccgc ccacaagaaa taccggcgtc ccttctttgc
     4081 ctgcgcgcgc catcagctct tcccagagat cggcaccggc aacgcgggaa acctgcgcct
     4141 gcgggtactt tttacgtact gaacgtacaa tgctgatgcc atccgcatat ttaaattcgg
     4201 cagcgttaat taactccctg acctcggcgt tatcttcaat ggtcagcatt ttttcggcat
     4261 taatggcaac cagcgttccc tgcttaagct gcccgtcagc aaacagataa tcgagggcat
     4321 gctgcatatc acgccaacca atcaactgta agccacgcag cgtataggtc ggtgccgtgg
     4381 tgttgttatt cattgttatc cttcaacctg cgtccggagc gatgattttg tacgtttatg
     4441 aatgagtccg gcgctttcaa aaagccagta caacagtttt gcgatcatca gacatgcgcc
     4501 gaagaccacg ataaaaaaga ctacgcgtga gacaaacgaa tccagccctt cacgtgccag
     4561 cacgatcata ttgaagatcg ccccaaagca gaaactgtgc aatatcgcag ccttatagcg
     4621 attagtctcg cggttgccca gctcatacag ccagtcgaac catttgatga tcagtccaac
     4681 cacaatcgcc ccgagtggga tgaacaacgc gccgcccatc accaccagtg agcctataag
     4741 cgtaggcgag atcgccagtc cggagtggtt attcagtact tcccaggtaa agtaattggc
     4801 tgagttcagc accatactcg ggcgccccgg ccacagccag gaagggataa agacatagaa
     4861 atcgcggaca attggagcca ggccctggaa gtcgatgttg tcgtagttct gcaacagcaa
     4921 cgccagattc tcccacggtg aaaaggtgtc gcgagtgaga tagagaaacg tatagaacgc
     4981 ttcatcgccg ctcacattca ttccatagcg ttttaacgcc agccagaaca tgccaacaat
     5041 ccccagcacg cccgccgccg ccagcatcca caacgaaatc cagccgcgaa taatgccaat
     5101 aaacaggaag atagcgaatg cgatgatgat attggcgcga gtgccgccga caatcatata
     5161 agtcagcaag ccaaaggcga ccgtgctgac gaggaaaaac agccacgctt tgctgtcctg
     5221 gcgcagaaag tagaccacca gcatcgccgg gatgaaaaag taaaagaagc gttttaacgc
     5281 cacgccggag acttcactgg aaaagatctg actgtaggag ttaagccgga acagcaaaaa
     5341 gccgttgtgc atgaagaaga tgccgacgct taccagcgcg atacccatca ggatcaccca
     5401 cgtaagattg gtctccacgc ggttcatggt aaacagcgga cggcgcggta catcagcaac
     5461 gcgtttgcgt aggcgggttt tgtaggtgac atagtaaacc gcgtagaagc agcccgcaga
     5521 aagcaacacc tgcaacaaga tttctggcgg cgcgacacca acatcaaagc gaaataccag
     5581 cacgctggtc agcgggaagc cgaagaaaaa ggtgagcaaa aacagcaatg aaaagaagac
     5641 attgaagtta aagcgcacac ggcgaaactc aaaccaggtc agcgtggcaa taaacagcgt
     5701 gcagagcagc cagaccacaa acaggccact gaattgcagc agactcatgc gacctccccg
     5761 gcggcaatcg ccaacgcccg ctgccagcct tgtagatagt tagggctaaa gaaggcaatg
     5821 gtgtttttat ccgccgacgc caactggcgc tgcgcttcac gcacaatatc ctcgttgaga
     5881 tcgtcggtag taaacagcac tggcaaatgc tgttccgtca tatcctgcca gaacggattt
     5941 tccctgttaa gcacacaagg aatgcccgcc tgaatcagta agcaaagcgt accaatgccc
     6001 tgctggcggg caaaaataaa gtaaccaaga tcgcactgac gaagtagcgc cagataggcg
     6061 tcaaattcca gtttttcgct caaaatttgc agattttctt cgctgaataa ctccagcccc
     6121 gcctgacgaa cttcctcaat gtacgcttcg ttattaggcg gatatcccat cggcaccacc
     6181 acttttaccg tatcgccaaa ttgctgatga acggcacgta aagcggcgac atgctcattg
     6241 ctgcggtcgc cggagttccc caccagaatg gtcatttttc cttcacgttg ccgatcgttc
     6301 gccatcgtat tgagagaagg gtccatccgc gtcgggaagt acagcagttc tccccgcacc
     6361 tttgggtgtg ttttggcaaa aaagctcaaa tcgccgcggg tggcaaatac acagccgact
     6421 cgcttttgcg ccaggcgacg tagtgggtaa aaaagcttat atctcaagcc actggaaagc
     6481 tcatacaggt ctgcccccca gatatgccag aaaaactggc tgggcttaat gccaccactc
     6541 agcagagcca gccacagtgt gggattgaac tgaccgtgga aaaaaaatcg ctgctgacgg
     6601 ttagcttttg ctttcgcgat gaccgcttcc gccagcgatt ttttcccagg gaaaaattgc
     6661 acagaaagcg ccggacagct atcacttaag ccgtcgtcct tgccaacaac cataaactcg
     6721 cgcgcgtgct cgctcgtcgc ggccagcgcg tcattgaaaa accgcaaaac ggttcggtta
     6781 tggtgaggga tatccgatcc cagtacgtga atcagtacag tcatgcccgc ctacgccaga
     6841 gtaaaaacac gccacaacaa agagaaaaat agacgatata agttgccata tatgcctgcg
     6901 ccgcgcccag tgcaccatgc gcagggatta gccagtgggc aaataccatc aataaagtga
     6961 actggctgac ttccgccaga atataaaacc gcagtgacgc tttggcgatc accagataac
     7021 caaagacata agcgcccact tttaacacat cacccactaa ctgccaggca aagagatcgc
     7081 gcatagcggt aaatttattc gacaacagca accagatagc aaaatcacgc agcaaccaga
     7141 cggtaaaact cgctgccgcc actgccggta agacgaattt cagcgattta accacttccc
     7201 gggtgatatc gcgcttttcc gttagccgcg acaacgtggg cagcaaatat acgctgaacg
     7261 atgccgtaat aaattgcagg taggcatcgg aaatactgct caccccttgc cagatcccca
     7321 cttcatccca gctatactgc gccgccagca gtttacgcat catgatgtaa gcaacaggca
     7381 aggtcaccga cgtaatcaac gccatgagcg taaatttgct caactgccct gccagaccgt
     7441 tatcccagct gggtttcaga tagcttaacg ggatgacacc acgtttaatc aacatgatgg
     7501 cggcaggaat taccaccagc gcgggaatca gcgccagacc cagcaacgcc ccttcataac
     7561 cgcccaaacg gtaactgacg tagtacgcga gaacgccaat caagctgccg acaatcagcg
     7621 ataacgcatt acctgcggca tcgcgaaagc ctttcatcag cgccagtaac aggttgcccc
     7681 aggcgatccc catttgcacc agcgccacta aacgcaccag cccctgatag tcggtattac
     7741 caaacaatcc ctggctgatt ggcgcagctg ccagcacaaa aaccagcgcc atcagcgtag
     7801 agaaaccaag taccatcgct gatgaagtgc cgaccacgcg gcgcagctgt tgcggattat
     7861 catggtactg ggcaacgtat ttggttacac cgttaaagat gccagccccg gcaagcacgc
     7921 cgagcacggt aatcaactgg cggaaatttg ccgccagccc aagccccgcc ggaccaaatg
     7981 acaccgccag caacttaccg accagtaacc cggcaccaat cttgaccagt gtactggccg
     8041 ccgtccacaa ggacgctttt gccaacgaca tatcaggaga agtagttcaa caaagtcgca
     8101 attaccgtac gctgattgac gggcgacagg ttgtagaaca gcggcaggcg cagcaggcgc
     8161 tcgctctctt tggtggtgta gcgatcttca ccgtggaact caccaaagtg ttcccccgca
     8221 gggcaaccgt gcagcggaat gtaatgaaac accgccatga tttccgcttc tttcagaaag
     8281 ttaatcaacg cgctccggtc atcaatatcc cgcagtttaa tgtagaacat atgcgcgttc
     8341 tgcacgcagc catcgggaat cgacggcagc tcgatacgcc cggctttcgc cagaggcgct
     8401 aacgtatcgt agtagttttg ccacagcgcc agacgttgct ggttgatacg atccgctgct
     8461 tccagttgcg cccacaggta cgcagcttgc agatcggaca tcaaatagct ggagccaata
     8521 tcgcgccagg tatatttatc gacctgacca cggaagaact ggctgcggtt agtgcccttt
     8581 tcacggatga tctcggctcg ttcgattaac gctttatcgt taatcagcgt cgcgccgcct
     8641 tcaccacccg ccgtgtagtt tttggtttca tggaagctaa agcagccaat atgaccaatg
     8701 gttcccagtg cacgcccttt gtaagtggac atcacgccct gagcggcatc ttccaccaca
     8761 aacaaattat gctttttcgc caacgccata atggtgtcca tttcgcaggc cacacccgcg
     8821 taatggaccg gcacgataac gcgcgttttg tcggtgatcg ctgcttcaat caacgtttcg
     8881 tcgatgttca tggtgtccgg gcgaacatcc acaaagacga ttttcgcgcc acgcagcaca
     8941 aaggcattgg cggtggagac aaaggtgtag ctcggcatga tcacttcatc gccaggctgg
     9001 atatcgagca gcagcgccgc catctccagc gaagcggtgc aggacggcgt cagtaacact
     9061 ttggcgctgc caaaacgttg ctccagccac tgctggcagc gacgggtaaa accgccatcg
     9121 ccacacagtt tgccgctacc cattgccgac tgcatatagt cgagttcggt tcccaccacc
     9181 ggcggtgcgt taaatggaat catgtgatca cctgtataac cagtacgcgg tgctttctac
     9241 attcgcacca ctttgtatgt atcgtttaag cgcggcggtg ttgcccattt gggtcgccac
     9301 ccgcaaagtt gttttaccgc gagcatacgc ccagtttagc gccgtttgca tcagctcagc
     9361 acctgcaccg cgtccagcca gcaggccaat tcgcgcatct gtcgcattga gttcccgtaa
     9421 agagacatag ccgcgaatat cgccggacgc cgcacgtaga atcagacatt gatgatcaaa
     9481 ggtgccgcgc acggcatttt caatccactg tgcataaaag cgaccgctgg cgtcaggcgc
     9541 ataccacggc gcacgaaaac ggctttgcgc aaatgcggcg ctagctaact gacgtaatgc
     9601 gggaatatcg gtctcttgtg ccacgacagc accgctatca ctgacattgt tcacgggtag
     9661 cgccaaatca acttcacctt ctaccaggga gaatcccagc tgttgcaggg catccagttc
     9721 ccccgtattt gatgccgcaa ttttggcctg cacccgtgac catggcgcta aggcgtctga
     9781 cgtcaggagc ggtgcttcag acgtaatgcg cacgatggcg ctgttaacac caaagaaggc
     9841 gttttcccag gttagtggct caatactggc gcggacgggc acgaagtaac tccagcagat
     9901 attggccgta gccagttttc gctaatgaac tggcagcacg cttcacaccc tcgtcatcga
     9961 gccagccgtt acgccaggcg atctcttcca ggcaggcaat cttaaagccc tggcgttttt
    10021 ctactgtctg cacaaaagtg ctggcctcaa tcaggctgtc gtgagtgccg gtatccagcc
    10081 aggcaaatcc gcgcccgagc agttcaacgg tcaggttgcc cgcctcgagg tacatctggt
    10141 tgatggaggt aatctccagt tcaccgcgct ccgacggctt cacctgcttt gcgtactcca
    10201 cgactttact gtcgtagaaa taaagcccgg tcaccgccca gtttgacttc ggctgttttg
    10261 gcttttcttc aagcgagata gcgcggaaat tgtcgtcaaa ttccaccacg ccaaagcgtt
    10321 ccgggtccat cacctgatag ccaaaaaccg tcgccccttc cgtgcgcgcc gcaacatgac
    10381 gcagcttcgg actgaagccc tgaccgaaga agatgttatc gcccagcacc agacaagaag
    10441 gttcaccatt aaggaaggtt tcaccgatga taaaggcctg cgccagaccg tccgggctgg
    10501 gctgttcggc atattccagc tggataccga actcactacc atcgcccagc aggcgctgga
    10561 aataaccttt atcttccggc gtagtgatga tgagaatttc gcggataccg gccagcatca
    10621 gcaccgacag cggatagtaa atcattggct tatcgtaaat cggcaacagt tgcttcgata
    10681 cgccgcgcgt aatcggatgc aatcgggtac cggaaccgcc cgccaggata atacccttca
    10741 tgtttgcctc cgctgaaaat tagcctttca gacctaaacg ctcaccctga tagctgccgt
    10801 cctgcacctg cttccaccag ctttcattag ccagatacca ctgaaccgtt ttacgcattc
    10861 cactttcaaa ggtttcctgc ggcagccagc caagttcacg ggcaattttc gaagcatcaa
    10921 tggcataacg cagatcatgc cccggacggt cagcgacaaa ggtgatcagg tcacgatagt
    10981 acgccacgcc gtgcggcttg ttcgaagcca gttcttccag cagctcgcaa atggtttcca
    11041 caacatcgag attcttacgc tcgttgtgac caccaatatt ataggtttca ccgactttcc
    11101 cggtggtcgc cacgcaatac agcgcgcggg cgtgatcttc cacatacagc cagtcacgga
    11161 tttgctgccc gttgccatat accggcagcg gtttacccgc cagcgcgttg aggatcatca
    11221 gcgggatcag tttttccgga aagtggtaag gaccgtagtt attcgagcag ttggtgatca
    11281 gcgtcggcag accgtaggtc cgcagccagg cgcgcaccag atggtcgctg ctggcttttg
    11341 acgcggaata agggctgctc ggcgcatacg gcgtggtttc ggtgaagaaa tcatccgtcg
    11401 agtgcaggtc accatatact tcgtcggtgg agatatgatg aaaacggaac gctgattttt
    11461 tatcttccgt cagcgtattc cagtaagccc gcgccgcttc aagcaatgta taagtcccga
    11521 caatgttggt ttcaataaac gctgccgggc cgtcaataga acggtcaaca tggctttcgg
    11581 ctgccagatg catgacacag tctggctgat gctcagtgaa tacgcgtgcc agttctgccc
    11641 gatcgcagat atcaactttc tcaaaggcaa agcgctcgct ttgcgcgacc ggtgccagcg
    11701 acatcaggtt tccggcgtag gtcagcttat cgaccactac caccgcatcg ctcgtttcgt
    11761 tgatgatata acgcaccagc gccgagccaa taaacccggc accacctgtt atcagaattt
    11821 ttctcatcag cgccagactc ctttggcatc gacgacatac tgctgatgga cattgtcgcc
    11881 attgataact ttgaactgac tatgatcgac cagcatcacc agcacgtctg ccgttgccag
    11941 cgcctcgtca agctgcgcca gagtacaaag cccggtcagt tttttcggca actggtggat
    12001 gttaggctca acaaccagcg tttcgccgct gtgccactgg gcgatcagtt cagcgatttc
    12061 catcgccggg ctttcgcgca ggtcatcaat attcggttta aacgccagac caaagcaggc
    12121 gattttcagt tcactggctc gtttatcggt agcagcaagg cagtcggcaa ccgccgcttt
    12181 cacctgatcg ataacccaga acggtttgtg atcgttaact tcgcgcgcgg tacggataag
    12241 ccgcgcctgc tgggggttct gtgccacgat aaaccacgga tcaacagcaa tgcagtgacc
    12301 gcccacgcca gggccaggct gaagaatatt aacgcgaggg tgacgattcg ccaggcgaat
    12361 cagttcccag acgttaatcc cctgatcggc acaaatcagc gacaattcat tagcaaaagc
    12421 gatattcaca tcgcggaagc tgttttcggt gagcttacac atttccgccg tccgcgagtt
    12481 agtgacgaca cactcacctt cgaggaaaat tttgtacaat tcgctggcgc gggccgaaca
    12541 aaccggcgtc ataccaccaa tcacgcgatc gtttttaatc agctcgacca taacctggcc
    12601 cggtaataca cgttccgggc agtaagcaat gttgacgtcc gcctgctcgc ccacctgctg
    12661 tgggaaagtc agatccgggc gcatctctgc taaccattct gccatcttct cggttgaccc
    12721 caccggcgag gtggattcaa ggatcaccag cgcgcctttt ttcagcactg gcgcaatgga
    12781 gcgagcagcc gattcaacgt aggtcatatc tggctcatga tcgcctttaa acggcgtagg
    12841 tacagcaatc agccaggcat ccgcttcaac tggcgtcgtg ctcgctcgta aaaaaccgcc
    12901 ttctacggca gtttttacta cgctcgccaa atcaggctcg acgatatgga tttcgccacg
    12961 attgatggta tcaaccgcgt gttggttgat atcgacacca atcacctgtt tttgccgtga
    13021 ggcaaacgct gctgctgtag gcagcccgat gtaacccagt ccgataacag aaatggtcgc
    13081 aaaactcata gtgatatccg attatttttt aacgcttcca gaatgcgaga gcatgcctga
    13141 ccatcaccat acgggttatg ggcgcggctc atagcttgat attcgttttc gtcttttaaa
    13201 agacgcgtca cttcctcgac aattcgctgc ttatccgtgc ctaccagacg caccgtaccc
    13261 gccgtcaccg cttccggacg ctcagtggta tcgcgcatca ccagcaccgg tttccccagc
    13321 gaaggcgctt cttcctgaat gccgcctgag tcagtcaaaa tcagccaggc gtggttcatc
    13381 agccagacaa acggtaaata ctcctgggga tcgatcagaa tgacattttt cacatgcccc
    13441 agaatgcgat tgaccggctc tctgacgttc gggttgagat gcaccggata gacaatctgg
    13501 atgtcctggt gcgtggtggc gatgtctgcc agcgcgtggc agatttcttc aaagccacga
    13561 ccgaaactct cacgcctatg accggtcacc agaatcatct ttttatcggg ggcgataaac
    13621 gggtaatttg ccgccagttc tgaacgcagc gtgtcgctgc tcatcacctg gtcacgcacc
    13681 cataacagtg catcaatgac tgtattaccg gtaatgaaga ttcggctatc cgcaacgttt
    13741 tcacgcagca agttttgccg ggaagtttcg gttggagaga agtgatacat cgccagatgc
    13801 ccggtcaatg tacggttagc ctcttccggc cacggcgaat agagagcgcc cgtgcgcaga
    13861 ccagcctcaa cgtgaccaac aggaatacgc tgataaaacg ccgccaggct ggttgccagc
    13921 gtcgtcgtcg tatcgccgtg aaccagcacg acgtctggtt tgaactcggc aagaataggt
    13981 tttagccctt ccagaatccg acaggttatc tctgtcaggc cctgtcctgg ctgcattatg
    14041 ttgagatcgt agtcaggtac aatggaaaag agtttcagta cctgatcaag catctcccga
    14101 tgctgcgcag tgacgcaaac tttagcctca aaaaaaggat cttttgccaa cgcatgcacc
    14161 aacggagcca tcttgatggc ttccgggcgc gtaccaaata cagtcagtac tttcacatcg
    14221 attctcttcg aataagcggc gagcgccttt gcgctcaccg cagcagtgtt gctatttcga
    14281 gcaacggcgg gttaatgcga caccagcccc gatcagcccc ccgacaatgc cccacataat
    14341 catcaggaag gcacgacgtg ggctatcgcg ttttaccggt tcttccggcg tacgcaaata
    14401 gcgataggtc tgaaaacgcg gatccagggt tggaccaaca ttcagggtgt ttaacatggc
    14461 tcgattctga tcatagtcga gatcaaaggc cggaccgacg gcctgtaaat tttccagtcg
    14521 agcctggagc attggacgcc caagcaggaa catttctgaa tcaggtaatt cctcggcagg
    14581 cacatctgtc gcactgcgcg aaatattatg ctgctcagca attttcagcg cctgctcaat
    14641 actgttcatc cggcggtcgt agatggcttt cgccacctct tcctgacgct tcacctgagc
    14701 tttcatctgg atggtgcgtg ccgcccatgc gcctttcagc tcatcattca gatggctggc
    14761 tgcacgctgg ctggcaaaag caacatactg acgtaacagg ttattagcgt caggcgcggt
    14821 ttcggcaata agcttcacgc tgtcattgac cgcgcgagta aagtctccgg ggataaactg
    14881 gatgttgtta atcatttcat ccagcaacgc cgcatcggct ttgctgttgc ccaccatccg
    14941 ctgtttgtaa tagtcggttt gcagccagaa ctctctgcgg gtatcccacg aggccagttg
    15001 cataacaaac tctttatagg cttcgtccat gaccgatggt tgatcggcag aagccatgtt
    15061 tgaacggaca tccaggttac gcaaaaattg ctgctgcgag taatatcccc ccagcatatt
    15121 caccgttgga cgatcggtaa tcgccgtcga gctccactcc tgacgagcaa aaaaagtata
    15181 cgccagcgcg attaacgcaa acgccagccc catgccaata atccatagct tcccagccca
    15241 caaggtacga aacaacccac gaatatccag ttcattttca gcgtcttcgg ccggtttccc
    15301 aggcattggt tgtgtcatca catcctcatt tatttggtta aattggggct gccaccacga
    15361 tttctacgca gtctgcgttt tacgcgctta ataaagcgag caactttcca ggcacgctta
    15421 atgcaatatc catagaggaa gaatgctagc aaaaagagca ccagcatgac ccactccggg
    15481 acaaaatgag aatattctgc cagcacgcca atggaagcga gcagtgctgc ggcaagggta
    15541 atcagcacaa acgcctgacg ggaagtaaac ccggcacgca tgatcaaatg gtgaatatgc
    15601 tgacggtcag gagagaatgg gctcatgcct ttacgcaggc gacggtacat aatcgccacc
    15661 atatccatta gcggaatggc gattatccac aaagcggtaa ccgggctgat gggatgggtt
    15721 ttgccctggg tcgtttcgag caggatccag ataacggtaa aaccaatcag cgtactgccc
    15781 gcatcaccca taaagacttt gtagcggcga cccaggatac caaggttaag catgatgtat
    15841 ggcaggatgg cggcgatcat cgcaaagcac cagattgcga ggctggtttg cccgtcgaac
    15901 cacaaaatca taccgattgc tgcaaacgag acgcaggaca acccgcccag caagccatca
    15961 atgccatcaa ccatgttgaa cgcattaatg gccgcccaga cggcaaatag cgtcaggaag
    16021 taaccaaacg gtccgagcac catctcccag gagccaaaga tataacccag gctactgaga
    16081 taaagtttgc cgaacaccat cataacaatg ccaacagcgg cctgtatggt ggcacggatt
    16141 tttacgctga tatcaaaacg atcatccagc gcgccaatga aaacaagcac accggcacaa
    16201 gcgagataga gagatgcatg cggaatatag taatcgacaa ttccgaacgt gaagcaaatc
    16261 cctgcgtaaa ccgaaatccc cccaacgaga ggtatcaatc cctggtgacg tttgcggaag
    16321 tttggtttat ccactaaacc gacttttttt gccaccttac gggcaaaaaa cagaaacagt
    16381 gtcgtgaata aaaaaatact gatgagatca gtactcactg tcagtaaatt cacaatgcat
    16441 gctctcagag aaaattatta gcagaagtat aaccacgaag acctttattc agaagggaaa
    16501 gtccgttccg aacccaccag ccctgtgcaa aaatcaacca tttgatatgc aaattgcata
    16561 acatgccagc aaattccagg aattttccta acatcgccag cgcgacatta agattacaga
    16621 tataaaagca aaacgccacg taaacacgtg gcgtttttgg cataagacaa atttatgagc
    16681 gtttcatcat ttcgaagaaa tcgtcattgg tcttggtcat ggccagttta ttaatgagga
    16741 attccattgc atcgatttcg cccatcgggt gaatgatttt gcgcaggatc cacattttct
    16801 gcagttcttc ctgagtcgtg agcagctctt ctttacgggt accggaacgg ttgtagtcga
    16861 tagccgggaa gacgcgtttt tcagcgatct tacgagagag gtgcagttcc atgttgcctg
    16921 tacctttaaa ctcttcgtaa ataacttcgt ccattttaga accggtatcg ataagcgccg
    16981 tcgcgataat ggtcaggctg ccgccctctt ccacgttacg cgccgcacca aagaagcgtt
    17041 tcggacgatg aagggcattg gcgtccacac caccggtcaa cactttacct gacgccggaa
    17101 caacagtgtt gtaagcgcgc gccagacgag tgatggagtc gagcagaatg ataacgtctt
    17161 tcttgtgctc aaccaggcgt ttggccttct cgatcaccat ttccgcaacc tgaacgtggc
    17221 gagatgcggg ttcgtcaaag gtagaagcaa caacttcacc ttttaccaga cgctgcatct
    17281 cggttacttc ttccggacgt tcgtcgatca gcagaaccat cagcacacaa tccgggtggt
    17341 tgtaagcaat gctctgagca atgttctgca gcagcatagt tttaccggct ttcggcggtg
    17401 ccacaatcag accacgctga ccacgaccga taggtgatgc cagatccagt acgcgagcag
    17461 ttaaatcttc agtagaaccg ttaccacgtt ccatacgcag acgagagttt gcgtgcagcg
    17521 gggttaagtt ctcaaagagg attttgttgc gggcgttttc aggtttgtcg aagttaactt
    17581 cgttaacttt cagcagcgca aaatagcgtt caccttcttt cggcgggcga atcttaccag
    17641 agatggtatc accagtgcgg aggttgaaac ggcggatttg gctaggggaa acgtagatgt
    17701 catcaggacc ggcgaggtag gagctgtctg cggaacggag gaaaccaaat ccatcctgca
    17761 atatctccag tacgccatca ccaaagatat cttcgccact ctttgcgtgc tgcttcagga
    17821 tggcaaaaat aatgtcctgc ttacgcatgc gagccaggtt ttccagcccc atattttcgc
    17881 cgagagtgat cagctcagaa accggcgtat tctttaattc ggtaagattc atagtggtgt
    17941 gagttcttaa acttggggta aatctcgaac ttaatgttgt gaatggtatg gcaggatcat
    18001 ccatgcctgt ttacggtcat cgactcatgt cttttcgctg cctggtcaca ggagagtacg
    18061 cagaactgaa acgacaagac ggattgagtg acgagccaga aatctgttca cttcgcattt
    18121 aagataaaac gggaagcgtt ggaaattaca agattcaaac ttaataaggt atgtttaata
    18181 cgaagtcaac actaagttag catgactcac gccgggcgtc cagtttttag cgacggggca
    18241 cccgaacatg aaattccctt acgccaggtt agcgtcgagg aactctttca actgaccttt
    18301 agacagtgcg cccactttgg ttgccgccac ttcaccgttt ttgaacagca gcagagtcgg
    18361 gataccacgg atgccatatt tcggcgcagt gccagggttt tgatcgatgt tcagttttgc
    18421 aacggtcagt ttgccctgat attcgtcagc gatttcatcc agaatcgggg cgatcatttt
    18481 gcacggaccg caccactctg cccagaaatc gacgaggatc gccccgtccg ctttgagtac
    18541 atccgtgtca aaactgtcgt cagtcaggtg aataatttta tcgctcatat ataactccac
    18601 aggaataagc ctggcgtgtt ggtttcgttg ttggtgtaac attaaccaac taaaggttga
    18661 ctttatttca ccggatacgc tttcgtaaag caatagtaag ctgatattct accacactat
    18721 gagcaaaaca catttaacag aacagaagtt ttccgacttc gccctgcatc cgaaggttgt
    18781 agaagccctt gaaaaaaaag ggtttcataa ctgtacgccc attcaggcac tggcccttcc
    18841 gctgacgctg gcgggtcgtg acgtagccgg gcaggcgcaa accggtaccg ggaaaacgat
    18901 ggcgtttctt acgtcaacgt ttcattatct tctctctcat cctgcgattg ccgatcgcaa
    18961 ggtgaatcaa ccgcgtgcct taattatggc accgacgcgt gaacttgccg tgcagatcca
    19021 cgccgacgcg gaaccgctgg cggaagctac cggcctgaag ctgggcctgg cttacggtgg
    19081 tgatggctat gacaaacagc tgaaagtgct ggaaagcggc gttgacattc tgattggcac
    19141 cacgggtcgt ttaattgact acgccaagca gaaccacatt aacctcggtg ccattcaggt
    19201 agtggtactg gacgaagccg atcgcatgta cgatctaggc tttattaaag atatccgctg
    19261 gctgttccgc cgtatgccgc ctgcaaatca gcgcctcaac atgctgttct ccgccacgct
    19321 ttcgtaccgg gtacgtgaac tggcgttcga gcagatgaac aatgccgaat atattgaagt
    19381 ggaaccggaa cagaaaacgg gccaccgtat taaagaagag cttttctacc cttctaacga
    19441 agaaaaaatg cgtttgctgc aaacgctgat cgaagaagag tggccagaca gagcgattat
    19501 tttcgccaat accaaacacc gttgtgaaga gatctggggc cacctggcgg cagatggtca
    19561 tcgtgtcggt ttgttgacag gcgatgtcgc gcagaaaaaa cgtctgcgta ttcttgatga
    19621 atttacccgt ggcgatctgg atattctggt tgccaccgac gttgccgcgc gtggtttgca
    19681 tattccggca gtgacgcacg tctttaacta cgatttaccc gatgactgtg aagattacgt
    19741 tcaccgtatt ggtcgtacag gtcgcgcagg cgcaagcggt cactctatca gcctggcgtg
    19801 tgaagagtat gcattgaatt tgcctgctat tgagacctat attggtcact caattccggt
    19861 aagcaaatac aatccggacg cattgatgac cgatctgcca aaaccgctgc gcctcacgcg
    19921 cccgcgcaca ggcaatggtc cgcgtcgtac tggcgctccg cgtaatcgtc gtcgttcagg
    19981 ttaaataaaa tgtcatactc aaaatcattc aaactgtatc aagatggcaa gagaatgaat
    20041 cccgatgagc ttactgtagt aagtgattcg ggtgaaagaa tgcagccaac acagagacag
    20101 attgaaggat gaagagtatg ggttccacct cgtcgctgta tgcagccatt gatctcggtt
    20161 cgaatagttt tcatatgctg gttgtgcgcg aggtggctgg aagcatccag acgctgacgc
    20221 gaattaaacg caaagtgcgt ctggctgctg gcctgaatag cgaaaatgac ctctctaatg
    20281 aagcaatgga gcgcggttgg caatgtctgc gcctgtttgc tgaacgtctg caagatatcc
    20341 ctccctcgca aattcgcgtt gtcgctacgg cgacattacg acttgccgtc aatgcgggtg
    20401 attttattgc caaagcgcag gaaatcctcg gttgtccggt acaggtgatc agcggtgaag
    20461 aggaagcgcg tctgatttat cagggcgttg ctcacaccac tggtggtgcc gatcagcgcc
    20521 tggtggtgga tataggcggt gccagtactg aactggtaac cggcacgggt gcacaaacca
    20581 cctcgttgtt cagcctgtcg atgggctgcg tcacctggct ggaacgctat tttgccgatc
    20641 gtaatctggg gcaagaaaat tttgatgctg cagaaaaagc ggcacgcgaa gtgttacgtc
    20701 cggttgccga tgaattacgg tatcacggct ggaaagtgtg cgttggtgct tccggcaccg
    20761 tgcaggcgtt acaggaaatc atgatggcac aggggatgga tgaacgcatt accctggaaa
    20821 agttgcagca attgaaacag cgagccattc attgcggtcg gctggaggaa ctggagattg
    20881 acgggctgac gctggaacgt gcattagtgt tcccgagtgg tctggcgatc ctgatcgcca
    20941 tttttaccga actgaatatt cagtgtatga ccctggcggg cggtgcgctg cgtgaaggcc
    21001 tggtctacgg catgttgcat ctggccgtcg agcaggatat tcgcagccgt acactgcgta
    21061 atattcaacg ccgctttatg atcgatattg atcaggcaca gcgcgtagcc aaagttgcgg
    21121 ctaacttctt cgatcaggtg gaaaacgaat ggcatcttga agcaataagc cgcgatttgc
    21181 tcatcagcgc ctgtcagctt catgaaatcg gcctgagcgt tgacttcaaa caagctccgc
    21241 aacacgctgc ttatctggtg cgtaacctgg atcttcccgg ttttaccccc gcacagaaga
    21301 aactgctggc gacgctactg ctcaaccaga ctaacccggt cgatctctca tcgctgcatc
    21361 agcaaaatgc cgtaccaccg cgcgtcgcag aacaactctg ccgtttactc cgcctggcta
    21421 tcatttttgc cagccgtcgc cgtgacgatc tcgtgccaga gatgacatta caggctaacc
    21481 atgaactgtt gaccttgacg cttccgcaag gttggctaac ccaacatccg ctgggtaaag
    21541 agattattgc tcaggaaaac cagtggcaga gctatgtcca ctggccgctg gaagtgcatt
    21601 aatgagtaag tgccggatgc gatgctgacg catcttctcc ggccttgatt atttccctcg
    21661 ttttgccgcc atcatcgctt tcagattcgc cagatggctt tgccctttct gcatccgttc
    21721 ttcggcactg accactttgc gctcctgttc ccaaatcaga tcatcctgcg gcagctccag
    21781 caaaaagcgg ctcggctccg ggcgcaccag ttcgccgtac tgacggcgtt ctttacacag
    21841 cgtaaaggtc aattccttct gggcgcgggt aatgccgaca taggccagcc gccgctcctc
    21901 atcgatatta tcttcatcga tactgctctg gtgcggcaaa aacccttctt ccataccgac
    21961 catgtagaca taaggaaact ccagcccttt tgacgcgtgg agagtcatca gttgcacctg
    22021 atccagctct tcttcactct caccacgctc catcatgtcg cgtaaagtaa agcgcgtcac
    22081 cacctgggtg agcgtcatcg gctcatccag ttcgctgcct tccagcatct ccgtcatcca
    22141 gctaaacagt tggttgacgt tcttcatgcg catttcggca gctttcgtgc tgggcgatgt
    22201 ttcgtacagc caggattcat aatccatgcc gtggatcaga tcgcgcaccg cggcaatcgg
    22261 ctcccgctcc gccagacgct ggatttctgc caaccagtga gtgaagcggg tcaatgcttc
    22321 ataaccacgt ccgctaagcg tctgactcag gcccatatca aagctggcgg taaacatgct
    22381 tttattgcgc gtcatcgccc actcacccag ctttttcagc gtcgccgggc caatctctcg
    22441 cttcggcgtg ttaacgatac gcagaaatgc gctgtcatcg tccgggttag tcagcacgcg
    22501 cagataagcc agcaagtcct tgatttcagg acgagagaaa aacgacgtac caccagaaat
    22561 tttgtacggg atgcggtttt gcatcaggaa tttttcaaac acccgcgact gatagttacc
    22621 gcgataaaga atagcgtaat ctttgtactg cgttttattg acgaagtgat gcgcaatcag
    22681 ttcaccagtg acgcgctcgg cttcatgttc ttcgttattc gcgcttaaaa cttttagctc
    22741 tgtgccgtaa cccagttcgg agaacagacg cttttcaaag acgtgcgggt tattggcgat
    22801 caggatgttc gccgctttca aaatacgtcc ggaagagcgg tagttctgct caagcttaat
    22861 caccttcagc gccggaaaat cctggctcag cagcaccagg ttttgcggac gtgcgccgcg
    22921 ccaggagtag atcgactggt catcgtcacc caccacggta aagcgcgcgc ggctgcccac
    22981 cagcagtttc accagttcat actggctggt gttggtgtcc tgatattcgt ccaccagcag
    23041 gtagcgaatt ttgttctgcc agcgctcgcg gacttcttca ttgcgttgca gaagtagcgt
    23101 cggcaataaa atcagatcat cgaagtcgag cacattacag gctttcaggt gtgcatcata
    23161 cagcccataa caatgggcaa aaatacggtc ccgctcgcca atcgcactgg ctgccgcctg
    23221 ggacggtgtt ttgagatcgt tcttccagtt agagatggtc gaaatcaact gttgcaggag
    23281 aactttgtca tcttcaatca gcccttcggt caactctttg agcaacgcca gctgatcggt
    23341 atcgtcaaac agcgaaaagt tcgccttcat ccccagcgcc gcatactcac gcttgatgat
    23401 atccagcccc agcgtgtgga aggttgagat catcagccca tgcgcctctt tgcgacccag
    23461 cgtctgtccg acacgctctt tcatctcgcg cgccgcctta ttggtaaagg tcaccgccgc
    23521 gatatgtcgc gcctgataac cgcacccgcg gatcagatgg gcgattttat tggtgataac
    23581 acgagtttta ccggaacccg cacccgccag caccaggcag gggccggtaa cgaattcgac
    23641 agcttgttgt tggccggggt ttagacgcat aggaaaatcg ctcaatgaaa gtcgggaggg
    23701 gaaaataaca gcgtggtagt atagccagcc tcatccttac cactcaaggc acgatcatgg
    23761 caaaaacagc agcagcactg catatccttg taaaagaaga gaaactggct ctggatcttc
    23821 tcgtgcagat taagaacggt gccgatttcg gcaagctggc gaagaaacac tccatttgcc
    23881 catcaggcaa acgcggcggt gatttaggtg aattccgcca gggtcagatg gttccggcgt
    23941 tcgataaagt tgtgttctct tgtccggtac tggagccaac cggcccgctg cacacccagt
    24001 tcggttacca catcattaag gtgctgtacc gtaactaata gcaaggcctt ctccgggaga
    24061 aggccttgaa ctctgaatcg ctattaaccc gcaacagcaa tacgtttcat atccgtcata
    24121 tagccgcgca gtttcttacc tacttgctca atcgcatggc tgcgaatcgc ttcatttaca
    24181 tcacgcagct gcgcgttatc taccgcacct tccggaatag ctttacccaa atcgcccggt
    24241 tgcagttctg ccataaacgg tttcagcagc ggcacacaag cgtaagagaa cagatagtta
    24301 ccgtactcgg cagtatcaga gataaccacg ttcatttcat acagacgctt acggacgatg
    24361 gtgttggcaa tcagcggcag ttcgtgcagt gattcatagt aagcagattc ttcgatgatg
    24421 ccggaatcaa ccatggtttc gaacgccagt tcaacgcccg ctttcaccat cgcgatcatc
    24481 agtacgcctt tatcgaagta ctcctgctcg ccgattttgc cttcatactg cggcgcggtt
    24541 tcgaaggcgg ttttgccggt ctcttcacgc caggtcagca gtttcttatc atcgttggcc
    24601 cagtccgcca tcataccgga ggagaattcg ccggagatga tgtcgtccat atgtttctgg
    24661 aacagcggtg ccatgatctc tttcagctgt tcagacaacg cgtaagcacg cagtttcgcc
    24721 gggttagaga gacggtccat catcagtgtg ataccgccct gcttcagcgc ttcggtgatg
    24781 gtttcccaac cgaactgaat tagtttttct gcgtatgccg gatcggtgcc ctcttccacc
    24841 agcttgtcga agcacagcag cgagccagcc tgcaacatac cgcacaggat ggtttgctca
    24901 cccatcaggt cagatttcac ttccgcaacg aaggacgatt ccagcacgcc cgcacggtga
    24961 ccaccggttg ctgccgccca tgctttagcg atcgccatgc cttcgccttt cggatcgttt
    25021 tccgggtgaa cggcaatcag cgtcggtacg ccgaatccac gtttgtactc ttcacgcact
    25081 tcggtgccag ggcatttcgg cgcaaccatg actacggtga tgtctttacg gatctgctcg
    25141 cccacttcga cgatgttgaa accgtgcgag taacccagcg ccgcgccgtc tttcatcagt
    25201 ggctgtacgg tgcgcactac atcagagtgc tgcttgtccg gcgtcaggtt aaccaccaga
    25261 tccgcctgtg ggatcagttc ttcgtaagta cccactttaa aaccattttc ggtcgcttta
    25321 cgccaggacg cgcgcttctc ggcaatcgct tctttacgca gagcgtagga gatatcgaga
    25381 ccagaatcac gcatgttcag accctggttc agaccttgtg cgccacagcc gacgatgact
    25441 acttttttac cctgaaggta gctcgcgcca tcggcgaatt catcgcgccc cataaagcga
    25501 catttgccca gctgtgccag ctgctgacgc agattcagtg tattgaagta gttagccatg
    25561 gtgattcctc gtgatgttgt gcttcttatt gttcggttcg cgtttgcgag attgaattca
    25621 ctatatgaca ggaaatttat tgcggaaatt gatatattca caacgtcaca ttgcaatttc
    25681 tgcaacgtca acattgaggg ctgcgccatt ggatttacgc gatctgaaaa ccttcctgca
    25741 tctggcggaa agccgccatt ttggccgcag cgcgcgggcg atgcacgtta gcccgtccac
    25801 gctctcacgg cagattcagc gcctggaaga agatctcggc cagccgctgt ttgtgcgcga
    25861 taaccgcacg gtgacgctga ctgaagcggg cgaagaactg cgcgttttcg cccagcaaac
    25921 gctgttgcag tatcagcagc tgcgccacac catcgaccag caagggccgt tgctctatgg
    25981 cgaattacat atcttctgct cggtgaccgc cgcttacagc catctgccgc cgattctgga
    26041 tcgcttccgc gcggaacacc cgtcggtgga gatcaaactc actaccggtg atgcggcaga
    26101 tgcaatggaa aaagtcgtca caggtgaagc tgatctggcg attgccggga agccagagac
    26161 tctgcccggc gcggtggcgt tttcgatgtt ggagaatctg gcagtggtgt tgattgcacc
    26221 cgcattgccc tgcccggtgc gtaatcaggt ttctgtcgaa aagccggact ggtcaacggt
    26281 accgtttatc atggccaatc aggggccggt gcgccgccgc attgagttgt ggttccgacg
    26341 caataaaatc agtaacccga tgatttacgc cacggttggc gggcatgaag cgatggtatc
    26401 gatggtggcg ctcggctgtg gcgtggcatt gttgccggaa gtggtgctgg aaaacagccc
    26461 cgaaccggtg cgtaaccgcg tgatgatttt agagcgcagc gatgagaaaa cgccgtttga
    26521 gctgggcgtg tgtgcgcaga aaaagcggct acatgagccg ctaattgagg cattctggaa
    26581 gattttgccg aaccattaac ccgccaaaaa gaacctgaac gccgggttat tggtttcgtc
    26641 gtggcaatcg tagcccagct cattcagccg ggtttcgaaa tccggttcat ggtcgccaag
    26701 ttcgaacgcc gccagtacgc gcccgtagtc ggtgccatgg ctgcgatagt ggaacaaaga
    26761 aatgttccag tgcgtaccca gcgtgttgag gaagcgcagc agcgcgcccg gtgattccgg
    26821 gaattcgaag ctgtagaggc gttcctgcaa cggatgtgac ggacgcccgc cgaccatata
    26881 gcgcacatgc aacttcgcca tttcgtcgtc ggagagatcc accacgctgt agccgccgtc
    26941 gttgagcatc tgcaaaattt ctttgcgctc ttcgaggccg cggctcaggc gcacgccgac
    27001 aaagatgcag gcgtttttgg catcggcaaa acggtagttg aactcggtga ccgatcgccc
    27061 gccaagcagt tggcagaatt tgaggaagct gcctttttct tccggaatgg tcaccgccaa
    27121 caacgcttca cgctgttcgc ccagttcgca gcgttccgag acgtagcgca ggccgtggaa
    27181 gttcacgttg gcaccggaaa gaatatgcgc cagccgttcg ccgcgaatgt tgtgctgggc
    27241 gatatatttt ttcattcccg ccagcgccag cgcgccagag ggttccgcca ccgcacgcac
    27301 atcttcgaat aaatccttca tcgccgcaca gatcgcatcg ctatcgacgg tgatgatgtc
    27361 gtcgagatac tcctggcata aacggaaggt ttcgtcaccg atgcgtttta ccgcgacacc
    27421 ttcggcgaac agtcccacgc gtggcagatc aaccggatga cccgcatcca gcgctgcttt
    27481 caggcaggcg gagtcttccg cttctacggc gatcactttg atttgcggca tcagttgttt
    27541 gatcagcacc gctacgcccg ccgccagacc gccgccgccg actggcacaa atacgcggtc
    27601 gagatgggcg tcctgctgga gcagttccag cgccagcgtg ccttgcccgg caatcaccat
    27661 cggatggtcg aacggcggca cccaggtgaa cccctgctgc tgtgacagtt cgatcgcttt
    27721 agctttcgct tcgtcaaagt tcgcgccatg aagcagcact tcgccgccga atccacgtac
    27781 cgcatcgact ttgatgtcgg cggtggcggt tggcataacg atcagggcct tcacgcctaa
    27841 ccgcgcagaa gaaaacgcga cgccctgcgc gtggttaccc gcagaagcag tgatcacgcc
    27901 gtgcgctttt tgttcttccg tcaggcccgc catcatggcg tatgcgccgc gcagcttaaa
    27961 gctgtgcact ggctggcgat cttcgcgctt caccagaatg acgttatcaa gacgcgacga
    28021 cagtttttcc attttttgta gcggcgtaac ctgcgccgcc tcgtaaaccg gcgcgcgcag
    28081 cactgctctt aaatattcgg caccttccgg agcaccggac aggggttgcg agtcagccat
    28141 tattaacccc ccagtttcga tttatcgcgc accgcacctt tgtcggcgct ggttgccagg
    28201 ctggcgtagg cgcacagggc aaaggaaacc tgacgttcac ggtttttcgg cgtccaggct
    28261 ttgtcacccc gagcttcctg cgcttcacga cgcgccgcca gttcggcatc gcttacctgt
    28321 aactgaatgc cacggttcgg gatgtcgata gcgatcaggt caccatcttc aatcagacca
    28381 atgctgccgc cgcttgccgc ttccggtgag acgtggccga tggaaagacc agaggtgccg
    28441 ccagagaaac gaccgtcggt gatcagcgca caggctttac cgagacccat tgatttcagg
    28501 aagctggttg ggtagagcat ttcctgcatg ccagggccgc ctttcgggcc ttcatagcgg
    28561 atcaccacca catcgcccgc gacaactttg ccaccgagga tcgcttctac cgcgtcgtcc
    28621 tggctttcgt acactttcgc cgggccggtg aatttgagga tgctgtcatc gacgcccgcg
    28681 gttttcacga tgcagccgtt ttcagcaaaa ttaccgtaga gcaccgccag gccgccgtct
    28741 ttgctgtagg cgtgttccag cgagcggata cagccgtttg cgcgatcgtc atcgagagaa
    28801 tcccaacggc aatcctgcga gaatgcctgt gtagtacgaa tgcccgcagg gcctgcgcgg
    28861 aacatatttt ttaccgcgtc atcctgggtc agcataacgt cgtattgttc cagagtttgc
    28921 ggcaacgtca ggccaagtac gtttttcaca tcacggttca gtaaccccgc gcgatccagt
    28981 tcaccgagaa taccgataac accaccagca cggtgaacat cttccatatg gtatttttgg
    29041 gtgctcggcg caactttgca taactgcggg actttacgcg aaagtttgtc gatatcactc
    29101 atggtgaagt cgatttccgc ttcctgcgcc gccgccagca ggtgaagtac cgtgttggta
    29161 gagccgccca tcgcgatatc cagcgtcatg gcattttcaa acgccgcttt gctagcaata
    29221 ttgcgcggca gtgcactttc gtcgttttgc tcgtagtaac gtttggtcaa ttcaacaatg
    29281 cgtttaccgg cattgaggaa caactgctta cggtccgcgt gggttgccag cagcgagccg
    29341 ttgcccggct gcgacaggcc cagcgcttcg gtcaggcagt tcattgagtt agcggtaaac
    29401 atcccggagc aggagccgca ggtcggacac gcggaacgtt caacctgatc gctctgggag
    29461 tcagaaactt tcgggtctgc gccctggatc atcgcatcaa ccagatcgag tttgattatc
    29521 tgatcggaca gtttggtttt cccggcctcc atcgggccgc cggaaacaaa gatcaccgga
    29581 atattcaggc gcagggaagc catcagcatc cccggggtga ttttgtcgca gttagagatg
    29641 cagaccatgg cgtcggcgca gtgggcgttg accatatact caacggaatc agcgatcagt
    29701 tcgcgagatg gaagtgaata aagcatcccc ccgtggccca tggcaatccc atcatccacc
    29761 gcaatggtgt tgaactcttt ggcaacgccg ccagccgctt caatttgttc ggcgaccagt
    29821 ttaccgagat cgcgcagatg gacgtgaccc ggtacaaatt gggtgaacga gttcacaacc
    29881 gcgataatcg gcttgccaaa atcggcgtcg gtcattccgg tggcgcgcca cagcgcacga
    29941 gcacccgcca tattacgacc atgagtggtg gtggcggaac ggtacttagg catactttat
    30001 ttactcccag tgtctgtctc gtaaatggga cggtgcgtgc cgtcccattt tttgtattta
    30061 ttgattaact tgatctaacc agccccattt atcttcggtt tcgccagtga agaggccgaa
    30121 gaaggcttgc tgaatgcgtt tggtaaccgg gccacaacgg ccttcgccaa tctgaatacc
    30181 gtctacgctg cgcactggcg tgatttctgc cgccgtaccg gacataaaga cttcatccgc
    30241 caagtacagg gattcgcgcg acagcacctg ctcacgcact tcaattccca gctctttcgc
    30301 cagtttgatg atggcatcac gggtaatacc cggcagcgcg gaggaggtga acggtggggt
    30361 gaacagcaca ccatctttca cttcaaacag gttttcgcct gcgccttcag agatataacc
    30421 gttcacatcc agcgcgatac cttcctgata accgtggcgg cgcgcttcgc tacccaccag
    30481 cagggaagag agatagttac caccggcttt tgccgcagtt gggatggtgt ttggtgctgc
    30541 gcggttccag gaggaaacca tcgcatcgat cccctgctcc agcgcttctg cgcccagata
    30601 cgctccccac gggaaagcgg cgataatcac atcggttgag tatcccgctg gcgggtttac
    30661 tcccatgcca acatcaccga cgaagatcag cggacggata taggcgctgg tgagattgtt
    30721 tttgcggatc acgtcacgac aagcttccat cagctcatca atgctctgtg aaaccgggaa
    30781 gcgatagatt ttggcggagt catgcagacg ctgcatatgc tcacgatggc ggaatacaac
    30841 cggtcctttg tgtgagtcgt agcaacggat gccttcaaaa accgaggtgc catagtgcag
    30901 cgcgtgcgac atcacatgca ccttcgcgtc ttcccagcga accatctccc cattgaacca
    30961 aatgtaatca gctttcttcg tggtcatttt tatattcctt ttgcgctcag gcgcggattt
    31021 gttgtgatgt ggttgtgctc tggcagatgg caacgtgtgc gacgtccacc agtttattta
    31081 actgactaaa cagtaagtcg accgaccgtg ggctggcaac ggtcaattcg atatttatat
    31141 tttgtgcatc gctggcggca gccatattca ttgagcagac gtggaaacca cgatgacgca
    31201 ccacgcgtaa aacacgttct aaggtttccg gattgaagcg agccgataca ttgacctgat
    31261 gttgcatcat gataatttct ccaacatttc tgaattactg gcacctggcg gcaccagcgg
    31321 ccagacgttc tcaagttcgt cgattgagac atgaagcagg tatggcccat cactgttcag
    31381 catggtgtcg agtgccgctt caacctggtc tttacgggtg atgtgttggc cagggatgcc
    31441 gaaggcgctg gctaacatga ggaaatcggg gttatcggta agggtggttt cgctgtatcg
    31501 ttcctgaaaa aacagttgct gccattgtcg aaccatccct aaccgttggt tatcgagtaa
    31561 gacgattttc aacggtaact gcttgcgttt tacggtgccc agctcttgca cattcatcat
    31621 gaaagagccg tcaccggaga tacagacaac ggtatcgttc ggtcgcgcga cttgtgcgcc
    31681 aactgccgcc ggtaaaccaa aacccatggt acctaagccg ctggaggtga tgaaattttc
    31741 cgggcgagtg tgggcgatgt gctgcgcagc ccacatctgg tgctgcccca catctgtggt
    31801 cacgacgcaa tccgcaggtt tacgatccga cagttgtttt aacaacaacg gagcgtagat
    31861 agcgtcaccg ggatggtcgt aacgccagga atgttcatca cgcagctgcg cgcagtgttg
    31921 ctgccagtca ttgatattta acggctgctg taatgctggt aacagagcat ttaaatcacc
    31981 ttgtaatgcc acatgtgcct gacgcagctt gttcatttct gccgggtcga tatccatatg
    32041 gataacactg gcgtgtggtg cgaaggtgtt cagtttgccg gtcacccggt catcaaaacg
    32101 tgcacccacg gcgatcagca ggtcacactc ctgcaccgcg aagtttgccg ctttggttcc
    32161 gtgcatcccc agcatgccca gatagtacgg ataatctgct tctactgcgc ccagcccttt
    32221 cagcgtacag gtggcaggca tttttgtggc agcgagaaat tcacgcaaag ccggaactgc
    32281 ctgcgccatc cccacgccac cgccaacgta cagcatcggt ttttgcgctt ttgccagcat
    32341 ctggcgcgct tgctcgactt cggcatgtgg gaaagtcact tcgttttcaa cggtggtgaa
    32401 ccacggttcc agatcgccgc tggctaactg gatatctttt gggatatcga ccagaaccgg
    32461 accaggacga cctgagctgg caacgtcgaa tgcttcagcc atgatgcgcg gcaactcttc
    32521 cagagactgc accagaaagc tgtgcttggt acaggctaac gacaatccca ggacatccac
    32581 ttcctgaaat gcgtcggtgc cgataaacgg tgcggacacc tgaccagtga tggcaacgac
    32641 agggatggaa tctaacagtg cgtccgcaag cccggttatc aggttggttg cgcccggacc
    32701 agacgtggcg atacatacgc cagttttacc ggtagcacgg gcataaccga tagccgccat
    32761 tgccgcaccc tgctcatgtc ggcacagcaa gtgctccacg ccgccgtcat acaatgcatc
    32821 gtaaaccggc ataattgcgc cacccggata accgaaaacg gtgttcacac cctgtgcccg
    32881 caacgcatgt accacccact gtgcgccatt catagttagt tccccgtcct gaatcttgag
    32941 aaacagaatt ttgtgctgtt attcattgtc tgctcctcgg ttatgttttt aaggtcaaaa
    33001 aaacccccgg acctttcggt gcgggggtct tagttcgtta aggcttgatc tctaagcctt
    33061 tcctcgtcca agtgcagccc cgcacggtgg gataataatc accaccacgc taatcacgac
    33121 caggctaatc actcgtagaa gggctgtcat tttgtctttt cttgcatctt gttcgaagga
    33181 atgcctaaag agttaccata gattttgcga atagcacaag atatttttgg gcctattttt
    33241 atgatggctt taataatttc aatagaaatt gttgtttttt aaaaagaaaa tagtgagtga
    33301 aaaaattcaa tttcagtata aatgttgttc ccttcctcgt agttcatcct ttgtccgctg
    33361 catttttgcg agcatcattc caccgccaga acaactcaac ttcacttgca caaataaaca
    33421 ggaatcagcc actaaaattc acgaattagc gggctacagc agcgatatct tatgcacata
    33481 atggtgactc aaggagggtt tatgtcactg tcaattgttc atacccgcgc agccctggaa
    33541 gtaaatgcgc ccccaatcac tgttgaggta catatcagta aaggtctacc cggcttaacg
    33601 atggtgggct taacagaaac aacggtgaaa gaagctcgcg atcgcgtgcg cagcgccatt
    33661 atcaatagcg gatatgaata tccggcgaaa aaaatcacca tcaaccttgc tccagccgat
    33721 ctgccaaaag aagggggacg atatgattta cctatcgcca ttgcgttgct ggcggcctca
    33781 gaacagctta cagccaataa gttagatgaa tatgaattag tcggagaact agcgcttaca
    33841 ggcgctctgc gtggcgttcc cggtgcaatc tccagtgcaa ctgaagctat taagtcgggc
    33901 agaaaaatta tcgtcgcgaa agataacgaa gatgaagtgg ggctaattaa cggtgaagga
    33961 tgcctgatag ccgatcatct gcaggctgtc tgtgcgtttc tggaaggtaa gaacactctc
    34021 gaacgcccga aaccaactga tgcagtatcc cgggcgctac aacatgatct cagtgatgtt
    34081 gtcggtcagg agcaaggaaa acgaggactg gaaattaccg ccgctggtgg gcacaacctt
    34141 ttactgattg ggccgccggg aacaggtaaa acaatgctcg ccagccgtat taatggcctg
    34201 ttgccagatt taagcaatga agaggcactg gagagcgctg cgatattaag tctggtaaat
    34261 gctgaatcag tacaaaaaca atggcggcag cgcccgttcc gctcacctca tcacagtgcg
    34321 tcgttaactg cgatggtggg cggtggtgca attccagggc caggtgaaat ttcgttggcg
    34381 cataacggcg tgctttttct tgatgagcta cctgaatttg aacggcgtac actggatgcc
    34441 ttgcgagagc cgattgaatc cgggcagatc catctttcac gcacacgagc aaaaataacc
    34501 tatccagccc gtttccagct tgttgcggcg atgaatccca gccctaccgg acattatcag
    34561 ggaaaccata accgctgcac gccagaacag acattacgtt atctcactgg agatcccatt
    34621 accacccccc ggcattttga gtaaaacggt agtgccggga gaaagcagcg ccaccgttaa
    34681 acaacgcgta atggccgcca gagagcgcca atttaagcgg cagaataaac tgaatgcctg
    34741 gctggatagt ccggaaatac gccaattctg caaacttgag agcgaagatg cgatgtggtt
    34801 ggaagaaacg ctgatccatc tggggttatc gattcgtgcc tggcagcggt tactgaaagt
    34861 tgcacgaacc attgctgata ttgatcagtc tgacattatc acacgtcagc atttgcagga
    34921 ggcagttagc tatcgtgcga ttgaccgttt gctcatccat ctgcaaaaac tactgacata
    34981 aaaaaagggc atttcgccct ttttattaat cgtcagaatc ggtgtagtct tcagcacctt
    35041 caacctgcgg tttaccgccg gaaagggtgt gaaaacgttt tggacgctta atacgcgtca
    35101 tatacttgga ccacacgcgt tctgcttctg tcactggctc acgttcgcca cggcatactg
    35161 ctacgaagag tttctcttcc tcggtaaccg gctcgcgttt gccaagatcc aactcattga
    35221 aggcataacc atgacgctca agcagttgtg cctctttgat ggtgaaatca ccatgacgag
    35281 agaatccacg tggataatgt ttattgtcga aatatcgatt agtcgtcgta aagctttccg
    35341 ccatcctgca cgctcctaat tctttgaccg agctagttat ggcgcggagt attagttacg
    35401 cttgacagag tgtaaaacaa aacatttaaa tcataacgac aaataatttt gcggagagca
    35461 ctgtggatac ggaattgtta aaaactttcc tggaagttag ccgaacgcgt cactttggtc
    35521 gagcggctga atcgctctat ctgacccagt cagcagtgag ctttcgaatc agacaactgg
    35581 aaaatcaact gggtgtgaac cttttcaccc gccacagaaa caatatccgt ttaaccgctg
    35641 ccggtgaaaa actactgcct tatgcagaaa cgctcatgag cacctggcag gccgcccgca
    35701 aggaggtggc gcatacctca cgacataacg agttttctat cggtgccagc gcctcgttgt
    35761 gggaatgtat gcttaatcag tggttgggac gcttgtatca aaatcaggat gcccatacag
    35821 gcttacagtt cgaagcgcga attgcccaac ggcagtctct ggtaaaacag ctgcatgaac
    35881 gccagcttga tcttcttatt accactgaag cgcccaaaat ggacgaattt agtagtcagt
    35941 tgctggggta tttcacttta gcgctttata ccagtgcccc ttctaaacta aagggagatc
    36001 ttaactatct gcgactggag tgggggccag attttcaaca gcatgaggca ggtttgatcg
    36061 gtgctgacga agttcccatt ctgacaacca gttctgctga actggcacag caacagattg
    36121 cgatgcttaa tggttgcacc tggctacccg tcagctgggc gcgtaaaaaa ggcggcctgc
    36181 ataccgttgt cgatagcaca acactttcac ggccgcttta tgccatatgg ctgcaaaata
    36241 gcgataaaaa tgcgttgatt cgcgatcttt tgaaaattaa cgtgctggat gatgtgtatt
    36301 aatctgaatg gctggcaagg atgccggtag aaggatttac ttcggagagg attatttcag
    36361 ataaaaaaaa tccttagctt tcgctaagga tgatttctgg caggggcgga gagactcgaa
    36421 ctcccaacac ccggttttgg agaccggtgc tctaccaatt gaactacgcc cctaattagg
    36481 gtggcggaac ggacgggact cgaacccgcg accccctgcg tgacaggcag gtattctaac
    36541 cgactgaact accgctccac cgaattcttt tacaaccacc ggtttta
//
LOCUS       DAZPCS010000026        34999 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZPCS010000026 DAZPCS010000000
VERSION     DAZPCS010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34999
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.026"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..815)
                     /locus_tag="ACHUQ7_001271"
     CDS             complement(<1..815)
                     /locus_tag="ACHUQ7_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR1134764.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSS"
     gene            907..993
                     /locus_tag="ACHUQ7_001272"
                     /pseudo
     CDS             907..993
                     /locus_tag="ACHUQ7_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1003..1083)
                     /locus_tag="ACHUQ7_001273"
                     /pseudo
     CDS             complement(1003..1083)
                     /locus_tag="ACHUQ7_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1398..1874)
                     /locus_tag="ACHUQ7_001274"
     CDS             complement(1398..1874)
                     /locus_tag="ACHUQ7_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFR1134765.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1933..3222)
                     /gene="bioA"
                     /locus_tag="ACHUQ7_001275"
     CDS             complement(1933..3222)
                     /gene="bioA"
                     /locus_tag="ACHUQ7_001275"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFR1134766.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3309..4349
                     /gene="bioB"
                     /locus_tag="ACHUQ7_001276"
     CDS             3309..4349
                     /gene="bioB"
                     /locus_tag="ACHUQ7_001276"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFR1134767.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFEPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLGQALMT
                     PDTDEYYNAAAL"
     gene            4346..5500
                     /gene="bioF"
                     /locus_tag="ACHUQ7_001277"
     CDS             4346..5500
                     /gene="bioF"
                     /locus_tag="ACHUQ7_001277"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFR1134768.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5487..6242
                     /gene="bioC"
                     /locus_tag="ACHUQ7_001278"
     CDS             5487..6242
                     /gene="bioC"
                     /locus_tag="ACHUQ7_001278"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFR1134769.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            6235..6912
                     /gene="bioD"
                     /locus_tag="ACHUQ7_001279"
     CDS             6235..6912
                     /gene="bioD"
                     /locus_tag="ACHUQ7_001279"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFR1134770.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAESTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            7491..9512
                     /gene="uvrB"
                     /locus_tag="ACHUQ7_001280"
     CDS             7491..9512
                     /gene="uvrB"
                     /locus_tag="ACHUQ7_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFR1134771.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMDELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDRSTPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMLPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(9704..10612)
                     /gene="yvcK"
                     /locus_tag="ACHUQ7_001281"
     CDS             complement(9704..10612)
                     /gene="yvcK"
                     /locus_tag="ACHUQ7_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFR1134772.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPNVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      10877..11021
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            11009..11998
                     /gene="moaA"
                     /locus_tag="ACHUQ7_001282"
     CDS             11009..11998
                     /gene="moaA"
                     /locus_tag="ACHUQ7_001282"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFR1134773.1"
                     /translation="MASQLTDVFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEGDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            12020..12532
                     /gene="moaB"
                     /locus_tag="ACHUQ7_001283"
     CDS             12020..12532
                     /gene="moaB"
                     /locus_tag="ACHUQ7_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFR1134774.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDEAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            12535..13020
                     /gene="moaC"
                     /locus_tag="ACHUQ7_001284"
     CDS             12535..13020
                     /gene="moaC"
                     /locus_tag="ACHUQ7_001284"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFR1134775.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            13013..13258
                     /gene="moaD"
                     /locus_tag="ACHUQ7_001285"
     CDS             13013..13258
                     /gene="moaD"
                     /locus_tag="ACHUQ7_001285"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFR1134776.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            13260..13712
                     /gene="moaE"
                     /locus_tag="ACHUQ7_001286"
     CDS             13260..13712
                     /gene="moaE"
                     /locus_tag="ACHUQ7_001286"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFR1134777.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            13849..14553
                     /locus_tag="ACHUQ7_001287"
     CDS             13849..14553
                     /locus_tag="ACHUQ7_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFR1134778.1"
                     /translation="MDRFPRSDSIVQPWAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            14758..15471
                     /locus_tag="ACHUQ7_001288"
     CDS             14758..15471
                     /locus_tag="ACHUQ7_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFR1134779.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWPGIISHGRNKLLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(15507..16463)
                     /locus_tag="ACHUQ7_001289"
     CDS             complement(15507..16463)
                     /locus_tag="ACHUQ7_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HFR1134780.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDYWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(16463..17704)
                     /gene="clsB"
                     /locus_tag="ACHUQ7_001290"
     CDS             complement(16463..17704)
                     /gene="clsB"
                     /locus_tag="ACHUQ7_001290"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFR1134781.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     AQREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVNP"
     gene            complement(17701..18462)
                     /locus_tag="ACHUQ7_001291"
     CDS             complement(17701..18462)
                     /locus_tag="ACHUQ7_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFR1134782.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            18595..19005
                     /locus_tag="ACHUQ7_001292"
     CDS             18595..19005
                     /locus_tag="ACHUQ7_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFR1134783.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(18967..20073)
                     /locus_tag="ACHUQ7_001293"
     CDS             complement(18967..20073)
                     /locus_tag="ACHUQ7_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1134784.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(20084..21217)
                     /locus_tag="ACHUQ7_001294"
     CDS             complement(20084..21217)
                     /locus_tag="ACHUQ7_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1134785.1"
                     /translation="MSNPILSWHRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(21210..22946)
                     /locus_tag="ACHUQ7_001295"
     CDS             complement(21210..22946)
                     /locus_tag="ACHUQ7_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR1134786.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(22939..23934)
                     /gene="hlyD"
                     /locus_tag="ACHUQ7_001296"
     CDS             complement(22939..23934)
                     /gene="hlyD"
                     /locus_tag="ACHUQ7_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFR1134787.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(23937..24608)
                     /gene="cecR"
                     /locus_tag="ACHUQ7_001297"
     CDS             complement(23937..24608)
                     /gene="cecR"
                     /locus_tag="ACHUQ7_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFR1134788.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            24837..26057
                     /gene="rhlE"
                     /locus_tag="ACHUQ7_001298"
     CDS             24837..26057
                     /gene="rhlE"
                     /locus_tag="ACHUQ7_001298"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFR1134789.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSVAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGIRLMNKRKTA"
     gene            complement(26051..26149)
                     /locus_tag="ACHUQ7_001299"
                     /pseudo
     CDS             complement(26051..26149)
                     /locus_tag="ACHUQ7_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(26231..26713)
                     /gene="ybiA"
                     /locus_tag="ACHUQ7_001300"
     CDS             complement(26231..26713)
                     /gene="ybiA"
                     /locus_tag="ACHUQ7_001300"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HFR1134790.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAARPIKVDGNTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            26833..28983
                     /gene="dinG"
                     /locus_tag="ACHUQ7_001301"
     CDS             26833..28983
                     /gene="dinG"
                     /locus_tag="ACHUQ7_001301"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFR1134791.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITVPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKYLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            29011..29973
                     /gene="ybiB"
                     /locus_tag="ACHUQ7_001302"
     CDS             29011..29973
                     /gene="ybiB"
                     /locus_tag="ACHUQ7_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFR1134792.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALCLSSVSHPEYIGRVAKFFSDI
                     GGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WTERCLAGSEAIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            30114..31199
                     /gene="hcxB"
                     /locus_tag="ACHUQ7_001303"
     CDS             30114..31199
                     /gene="hcxB"
                     /locus_tag="ACHUQ7_001303"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFR1134793.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            31266..31325
                     /gene="ybiE"
                     /locus_tag="ACHUQ7_001304"
     CDS             31266..31325
                     /gene="ybiE"
                     /locus_tag="ACHUQ7_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFR1134794.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(31428..31688)
                     /gene="ybiJ"
                     /locus_tag="ACHUQ7_001305"
     CDS             complement(31428..31688)
                     /gene="ybiJ"
                     /locus_tag="ACHUQ7_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFR1134795.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASPLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(31953..32219)
                     /gene="ybiI"
                     /locus_tag="ACHUQ7_001306"
     CDS             complement(31953..32219)
                     /gene="ybiI"
                     /locus_tag="ACHUQ7_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFR1134796.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDRQLR"
     gene            complement(32293..32970)
                     /gene="ybiX"
                     /locus_tag="ACHUQ7_001307"
     CDS             complement(32293..32970)
                     /gene="ybiX"
                     /locus_tag="ACHUQ7_001307"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFR1134797.1"
                     /translation="MMYHIPGVLSPQDVAHFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(33012..34999)
                     /locus_tag="ACHUQ7_001308"
                     /pseudo
     CDS             complement(33012..34999)
                     /locus_tag="ACHUQ7_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         8294 a         8972 c         9457 g         8276 t
ORIGIN      
        1 gaagaggtta atctttgcag ggcttgcaga gtgcgagtcg acaatggatt gttataaata
       61 ttaacatctg cataaatgtg cagattcatg atactgtctg gcaatgtgtt cagctgattg
      121 ttagggatat atagtgattg cagacgttgt ggaagttcgg gcagtctgtg tagttggttt
      181 tccccaacgt ttaaaagcct cagattttga ggaaggggcg gtagctcttg taatctgtta
      241 tcaaaagcac ttagttcctg tagttcacat ggtaattctg gtaaggcagt aagctggttg
      301 ttgccaacag aaagtttttg tagagccggg ggaagatcag gtagcgtctc caggctattg
      361 agaagggctg agagtgactg caaggaagat ggaagagaag gcaaacatgt taaaactctg
      421 ttaagtgaaa catcaagagc gaccaggtga ggcggaagag ccgggagcct gctcagtctg
      481 ttatcgctgg ctttaagtac agttaaggac ggagggagtt ctggcagaga gcgtagctca
      541 ttgctagaga tgttaagttc ttgtatgtgc gggggcaggt atgggagaga gcgtaatctg
      601 agtaaactta aattgagggc tggctcttga aaagccagac atattttcag taatcgaact
      661 gcctgtgttc ggtcttctgt tgcagcacct tccttggccc agttatccca gatgcggtga
      721 taatccgtga tattttgctc ttctacggat aaacgggata tacatcctaa accacaaaag
      781 gagcggaaaa aagaaggatt gtgggcaggt ttcatttatt attgatcctc attgtttata
      841 tattaatata ttcgtgcaaa tggagtgagg attctttaaa aaaacgcgcc tgtaaatagt
      901 taagaacagg aagaactgtt ggggatgtgg ctggccgaaa atgaaggggt gaagttctgg
      961 ctcaatgtgc tgactgaact gaaaaaacgc ggttggtcgc cctacctttt catcaagcca
     1021 gtagtctgcc caccacagca tcatttctct gcgcttatcg agatactgag catggttgta
     1081 aattcagcta acccagtgcg ttgacaaaaa attagcgcaa gaggacaaaa aatcaccttg
     1141 cgctaatgct ctgtatcagg tcactaatac catctaagtg gttgattcat agtgactgga
     1201 tatgttgtgt tttgcagtat tatgcagtct attttttaag ctaaatgtat tataatgcat
     1261 tgatattaat gatttttact gtttctcggt cagctttttt atactaactt gagcgaaacg
     1321 ggaaggtaaa aagacaaaaa gttgttttta atacctttaa gtgataccag atggcattgc
     1381 gccatctggc agagtgatta actaaacatc gcggtaatcg atgcgcttgc cagagagtgg
     1441 aaatgaacgt taaacccgac catcgcgccg ctggctcctt catcgacatc aatacgctct
     1501 acatccagcg cgtgaacggt aaaaatgtag cgatgggttt cgcctttcgg cggcgctgcg
     1561 ccatcgtatc cggttttacc aaaatcggta cgcgtctgca aaacgccgtc tggcattgct
     1621 accagaccag agccaaaccc ttgcggtaat acgcgggtat cagcgggtaa attaacaact
     1681 acccagtgcc accagccgga gccggttggc gcatccgggt cgtagcaggt gacaacaaaa
     1741 cttttcgttc ccgcaggaac atcatcccac gccagatgcg gtgaaatatt atcgccatcg
     1801 taacccatgc cgttaaagac atgacgatgc ggcaacttat cgccatcgcg cagatcgtta
     1861 ctgatgagtt tcatgaaccc tcctttcttg tttgcagaaa gtgtagccag aaaccctcac
     1921 gctgacttcc cgttattggc aaaaaaatgt ttcatcctgt accgcgcggt taaccgctgc
     1981 ggtcagacgc tgcaactgtt gcgggagaat aatatagggc ggcatcaggt aaatcagttt
     2041 gccaaaaggc cggatccata ccccctgttc gacaaagaat ttttgcagcg ccgccatatt
     2101 caccggacga gtggtttcga ccacaccgat tgcccccagt acgcgcacat cggcaaccat
     2161 ttcggcatca cgggctggtg ccagttgctc gcgcagctgc acttcaatag ccgccacctg
     2221 ctgctgccat tcgccggatt cgataatcgc taggctggcg tttgctgccg cgcaggccag
     2281 cggattgccc ataaaagttg gcccatgcat aaagcagccg gcttcgccgt tactgatggt
     2341 ttctgcaacc tcgcgcgtgg taagtgtggc ggaaagggtc attgtgccgc cggttaaggc
     2401 tttaccgagg cacaaaatgt ccggcgcgat ttctgcatat tcacaggcaa acagtttgcc
     2461 ggtacgacca aatccggtgg cgatctcgtc ggcaatcagc aagatacctt cgcgatcgca
     2521 cattttgcgg attcgtttta accattccgg atggtacatg cgcatcccgc ctgcgccctg
     2581 gacaatcggc tcaatgatca ccgctgcgat ttcatgacga tgcgccgcca tcaggcgggc
     2641 aaaaccaacc atatcgcgtt catcccattc accatccatg cggctttgcg gggcgggagc
     2701 aaacaagttt tctggcagat agcctttcca caggctgtgc attgagttgt ccggatcgca
     2761 caccgacatc gcgccaaagg tatcgccatg ataaccattg cggaaggtca gaaaacgctg
     2821 gcgcgcttcg cctttggctt gccagtactg caacgccatt ttcatcgcca cttccaccgc
     2881 tacggaaccg gagtccgcga gaaaaacgca ctccagcggt tgcggcgtca ttgccaccag
     2941 tttgcggcat agctcgatgg cgggcgcatg ggtgataccg ccaaacatca catgcgacat
     3001 ggcatcaatt tgcgtcttca tcgccgcatt aagctgcggg tgattgtagc cgtggatcgc
     3061 cgcccaccag gacgacatac cgtcaaccag gcgtctgccg tcagacaaaa ttagctcgca
     3121 accttcggcg ctcgccaccg gataaaccgg cagaggggag gtcatggatg tgtatgggtg
     3181 ccagatatgg cgttggtcaa aggcaagatc gtccgttgtc ataatcgact tgtaaaccaa
     3241 attgaaaaga tttaggttta caagcctaca ccgaattaac aacaaaaaac acgttttgga
     3301 gaagccccat ggctcaccgc ccacgctgga cattgtcgca agtcacagaa ttatttgaaa
     3361 aaccgttgct ggatctgctg tttgaagcgc agcaggtgca tcgccagcat ttcgaacctc
     3421 gtcaggtgca ggtcagcacg ttgctgtcga ttaagaccgg agcttgtccg gaagattgca
     3481 aatactgccc gcaaagctcg cgctacaaaa ccgggctgga agccgagcgg ttgatggaag
     3541 ttgaacaggt gctggagtcg gcgcgcaaag cgaaagcggc aggatcgacg cgcttctgta
     3601 tgggcgcggc atggaagaat ccccacgaac gcgatatgcc gtacctggaa caaatggtgc
     3661 agggggtaaa agcgatgggg ctggaggcgt gtatgacgct gggcacgttg agtgaatctc
     3721 aggcgcagcg cctcgcgaac gccgggctgg attactacaa ccacaacctg gacacctcgc
     3781 cggagtttta cggcaatatc atcaccacac gcacttatca ggaacgcctc gatacgctgg
     3841 aaaaagtgcg cgatgccggg atcaaagtct gctcaggtgg cattgtgggc ttaggcgaaa
     3901 cggtaaaaga tcgcgccgga ttattgctgc aactggcaaa cctgccgacg ccgccggaaa
     3961 gcgtaccaat caacatgctg gtgaaggtga aaggcacgcc gcttgccgat aacgatgacg
     4021 ttgatgcctt tgattttatt cgcaccattg ccgtagcgcg gatcatgatg ccgacctctt
     4081 acgtgcgcct ttctgccgga cgcgagcaga tgaacgaaca gactcaggcg atgtgcttta
     4141 tggcaggcgc aaactcgatt ttctacggtt gcaaactgct gaccacgccg aatccggaag
     4201 aagataaaga cctgcaactg ttccgcaaac tggggctaaa tccgcagcaa actgccgtgc
     4261 tggcagggga taacgaacaa cagcaacgtc ttggacaggc gctgatgacc ccggacaccg
     4321 acgaatatta caacgcggca gcattatgag ctggcaggag aaaatcaacg cggcgctcga
     4381 tgcgcggcgt gctgccgatg ccctgcgtcg ccgttatccg gtggcgcaag gagccggacg
     4441 ctggctggtg gcggatgatc gccagtatct gaacttttcc agtaacgatt atttaggttt
     4501 aagccatcat ccgcaaatta tccgtgcctg gaagcagggg gcggagcaat ttggcatcgg
     4561 tagcggcggc tccggtcacg tcagcggtta tagcgtggtg catcaggcgc tggaagaaga
     4621 actggccgag tggcttggct attcgcgggc gctgctgttt atctctggtt ttgccgcgaa
     4681 tcaggcagtc attgccgcga tgatggcgaa agaggaccgt attgttgccg accggcttag
     4741 ccatgcctca ttgctggagg ctgccagttt aagcccgtcg cagcttcgcc gttttactca
     4801 taacgatgtc gctcatctgg cgcgactgct tgcttccccc tgtccggggc agcaactggt
     4861 ggtgacagaa ggcgtgttca gcatggacgg cgatagcgcg ccactggcgg aaatccagca
     4921 ggtaacgcaa cagcacaatg gctggttgat ggtcgatgat gcccacggca cgggcgttat
     4981 cggggagcag gggcgcggca gctgctggct gcaaaaggta aaaccagaat tgctggtagt
     5041 gacttttggc aaaggatttg gcgtcagtgg ggctgcggtg ctttgctcaa gtacggtggc
     5101 ggattatctg ctgcaattcg cccgccacct tatctacagc accagtatgc cgcccgctca
     5161 ggcgcaggca ttacgtgcgt cgctggcggt cattcgccgt gatgaggggg atgcacggcg
     5221 cgaaaaactg gtgtcactca ttgcgcgttt tcgtgccgga gtacaggatt tgccgtttac
     5281 gctggctgat tcatgcagcg ccatccagcc attgatcgtc ggtgataaca gccgtgcgtt
     5341 acaactggca gaaaaactgc gccagcaagg ctgctgggtc acggcgattc gcccgccaac
     5401 cgtacccgct ggtactgcgc gactgcgctt aacgctaacc gctgcgcatg aaatgcagga
     5461 tatcgaccgt ctgctggagg tgctgcatgg caacggttaa taaacaagcc attgcagcgg
     5521 catttggtcg ggcagccgca cactatgagc aacatgcaga tctacagcgc cagagtgctg
     5581 acgccttact ggcaatgctt ccacagcgta aatacaccca cgtactggac gcgggttgtg
     5641 gacctggctg gatgagccgc cactggcggg aacgtcacgc gcaggtgacg gcgttagatc
     5701 tctcgccgcc aatgcttgcg caagcccgcc ataaagatgc cgcagatcat tatctggcag
     5761 gagatatcga atccctgccg ttagcgactg cgacgttcga tcttgcatgg agcaatctcg
     5821 cggtgcagtg gtgcggtaat ttatccacgg cactccgcga gctgtatcgg gtggtgcgcc
     5881 ccggaggcgt ggtcgcgttt accacgctgg tgcagggatc gttacccgaa ctgcatcagg
     5941 cgcggcaggc ggtggacgag cgtccgcatg ctaatcgctt tttaccgcca gatgaaatcg
     6001 aagagtcgct gaacggcgtg cgttatcaac atcatattca gctcatcaca ctatggtttg
     6061 atgatgcgct cagtgccatg cgttcgctga aaggcatcgg tgctacgcat cttcatgaag
     6121 ggcgcgaccc gcgaatatta acgcgttcgc agttgcagcg attgcaactg gcctggccgc
     6181 aacaacaggg gcgatatcct ctgacgtatc atcttttttt gggagtgatt gctcgtgagt
     6241 aaacgttatt ttgtcaccgg aacggatacc gaagtgggga aaaccgtcgc cagttgtgca
     6301 cttttacaag ccgcgaaggc agtaggttat cggacggcag gttataaacc ggtcgcctct
     6361 ggcagcgaaa agaccccgga aggcttacgt aatagtgacg cgctggcgtt acagcacaac
     6421 agcagcctgc agctggatta cgcaacggta aatccttaca ccttcgcaga atccacctcg
     6481 ccgcacatca tcagcgccca agagggcagg ccgatagaat cattggtaat gagcgccgga
     6541 ttacgcgcgc ttgaacaaca ggctgactgg gtgttagtgg aaggtgctgg cggctggttt
     6601 acgccgcttt ctgacacttt cacttttgca gattgggtaa cacaggaaca actgccggtg
     6661 atactggtag ttggtgtgaa actcggctgt attaatcacg cgatgttgac tgcgcaggca
     6721 atacaacacg ccggactgac tctggcgggt tgggtggcga acgatgttac gcctccggga
     6781 aaacgtcacg ctgaatatat gaccacgctc acccgcatga ttcccgcgcc gctgctggga
     6841 gagatcccct ggcttgcaga aaatccagaa aatgcggcaa ccggaaagta cataaacctt
     6901 gccttgttgt agccattctg tatttggtta aattgcgagc gagatcgcgt cttcgattga
     6961 ctgcaattta accaattaaa ttctaaaata atcacgaaaa aaattttact tccgcctcat
     7021 gcggcgaatg tgggaattgc ccaggcggcg ggggataggg gctggagaca gttatccact
     7081 attcctgtgg ataaccatgt gtattagagt tagaaaacac gaggcaagcg agagaatacg
     7141 cggcttgcac gcgaattggc gttaaagacg gctcaaagaa atatctttta ttttttaact
     7201 ggttagataa atgcaatggc agtcactgaa caggcatctc ttgccataaa actgtcatca
     7261 ctcatcttga caaatgttaa aaaagccgtt gctttgggga taacccggta aggctggagt
     7321 tttatctcgc cacagagtaa attttgctca tgattgacag cggagtttac gctgtatcag
     7381 aaatattatg gtgatgaact gtttttttat ccagtataat ttgttggcat aattaagtac
     7441 gacgagtaaa attacatacc tgcccgccca actccttcag gtagcgactc atgagtaaac
     7501 cgttcaaact gaattccgct tttaaacctt ctggcgatca gccagaggcg attcgacgtc
     7561 tcgaagaggg gctggaagat ggcctggcgc accagacgtt acttggcgtg actggctccg
     7621 ggaaaacctt caccattgcc aatgtcattg ctgaccttca gcgcccaacc atggtacttg
     7681 cgcccaacaa aacgctggcg gcccagctgt atggcgaaat gaaagagttc ttcccggaaa
     7741 acgcggtgga gtatttcgtc tcctactacg actactatca gccggaagcc tatgtaccga
     7801 gttccgacac cttcattgag aaagatgcct cggttaacga acatattgag cagatgcgtt
     7861 tgtccgccac caaagcgatg ctggagcggc gtgatgtgat tgtagtggcg tctgtttccg
     7921 cgatttatgg tctgggcgat cctgatttat atctcaagat gatgctccat ctcacggtcg
     7981 gtatgattat cgatcagcgc gcgattctgc gccgactggc ggagctgcaa tacgctcgta
     8041 atgatcaagc attccagcgt ggtactttcc gcgttcgtgg cgaggtgata gatatcttcc
     8101 cggcagaatc ggatgacatt gcacttcgcg tggaactgtt tgacgaggaa gtggaacgat
     8161 tgtcgttatt tgacccgctg accgggcaga ttgtttccac tattccacgt tttaccatct
     8221 acccgaaaac gcactacgtc acaccgcgcg agcgcatcgt acaggcgatg gaggagatca
     8281 aagaagaact ggccgccaga cgcaaagtgc tgttggaaaa caacaaactg ctggaagagc
     8341 agcggctgac ccagcgtacc cagtttgatc tggagatgat ggacgagctg ggctactgtt
     8401 cggggattga aaactactcg cgcttcctct ccggtcgtgg accgggtgag ccaccgccga
     8461 cgctgtttga ttacctgcct gccgatgggc tgctggtcgt cgatgaatct cacgtcacca
     8521 ttccacaaat tggcggcatg tatcgcggtg accgggcgcg taaagagaca ctggtggagt
     8581 acggcttccg cctgccatca gcgctggata accgtccgct taagtttgaa gagttcgaag
     8641 cattagcgcc gcaaaccatc tatgtttcgg cgacgccggg taattacgag ctggaaaaat
     8701 ccggcggcga tgtggtggat caggtggtgc gtccaaccgg attgcttgac ccgattatcg
     8761 aagtgcggcc ggtggcgaca caggttgatg atcttctttc ggagattcgt cagcgagcgg
     8821 caattaacga acgcgtgctg gtcaccacac tgaccaagcg aatggcggaa gatcttaccg
     8881 aatatctcga agaacacggt gagcgcgtgc gttatcttca ctcagatatc gacaccgtcg
     8941 agcgtatgga gattatccgc gacttgcgtc tgggtgagtt cgacgtattg gtagggatca
     9001 acttactgcg tgaaggtctg gatatgccgg aagtgtcgct ggtggcgatc ctcgacgctg
     9061 acaaagaagg cttcctgcgt tccgaacgtt cgttgatcca gaccattggt cgtgcggcac
     9121 gtaacgttaa cggtaaagcg attctctacg gcgataggag cactccatca atggcgaaag
     9181 cgattggcga aaccgaacgt cgccgtgaga aacagcagaa gtacaacgag gaacacggca
     9241 ttacgccgca aggcttgaac aagaaagtgg tcgatatcct ggcgctgggg cagaacattg
     9301 ccaaaaccaa agcgaagggc agaggaaaat cgcgcccgat tgttgagccg gataatgtgc
     9361 cgatggatat gttgccgaaa gcgttgcagc agaaaatcca tgaactggaa gggctgatga
     9421 tgcaacacgc gcagaatctg gagttcgaag aagcggcaca aattcgtgac cagttgcatc
     9481 agttgcgtga gttgtttatt gccgcatcgt aacaggatag cgaagaagac tgatgacaaa
     9541 cggaaaacag tctgatgcgc tacgcttatc aggcctacat tttctccgca atatattgaa
     9601 tttgcgcggt ttgtaggccg gataaggcgt tcacgccgca tccggcataa acaacgcgca
     9661 ctttgtcttc aataggaagc cggaattttc cttcggattt ccgttaacct aaagcctgta
     9721 acgccttttc cagcgcgtta tgtaacaact ggcggtcatg acgatacgga atatcgctgg
     9781 cctccagtac ctcctggatc acaatccgct ctttcaccgc cgagacatcc acttttggcc
     9841 cgacgatgac cgcatcaatg acctttttac caacatactg ctccataatt gccagcttgc
     9901 tttccagctt caaattagcc gcaggtaaac tcaactcacg ccccagattg ccgatataaa
     9961 ccatcggcgc tggcgtgcgg cgtaatgcct gggcgatttc cttcagcagc agaattggca
    10021 tcaggctggt ataaaaactg ccaggcccaa taatgatgag atccgcttca ttgatagcgt
    10081 gaaccgcctc acgcgttgcg ggtacattag gcgttaacaa taactcttga atcggcgtag
    10141 ttaactggtc gatattgacc tcgccgtaaa cttcatgccc ctgatcgtca atcgccatca
    10201 gatcaacagg atgctctgac attggaatca aatgcgtatc cactttcagc agattacgaa
    10261 ttaaattgat ggcttccaga ggccgcacgc taaggtgatc cagcgccttt aacatcaagt
    10321 ttccgagatt atgaccggaa agttcgccat tgccgccaaa acggtattca aacatcgcgg
    10381 aggcgacgtt cggttccgtt atcagctggt tgaggcagtt gcgcatatcg ccccaagcaa
    10441 tgccgccttc tgaacggcga atacgccccg tcgagccacc attatccgtg gtggtgacga
    10501 tacccgttaa acgagaaccc aaagacgaaa gtgatgagag aacgcgtccc agtccatgcc
    10561 ctccgccgag agcaacgaca cgatcaagat cagccagcgt acgattgcgc atatagtttc
    10621 ctggagtcag attatccgcg ctacagtagc gcaaattgcg gggaaacagc aattaacctg
    10681 ccaatattga ggatacaaac tattttcttc tatctctaaa ggacgatgca cgctatgcct
    10741 ccctgatgat gtatatcaaa gtaaaaccgc cattttccct tattctgtag cgaaatagca
    10801 cgatcatgac gctatataca tgattacata gcgaaagtgt ggatggtaaa aatctcattt
    10861 acacgctagt atcggcttaa ccactaaaca ctctagcctc tgcacctggg tcaactgata
    10921 cggtgctttg gccgtgacaa tgctcgtaaa gattgccacc agggcgaagg aagaaatgac
    10981 ttcgcctccc gtatctggaa aggtgtacat ggcttcacaa ctgactgatg tatttgcgcg
    11041 taagttttac tacttgcgcc tgtcgattac cgatgtgtgt aactttcgtt gcacctactg
    11101 cctgccggat ggctacaaac cgagcggcgt caccaataaa ggctttctta ccgtcgatga
    11161 aattcgccgg gttacgcgcg ccttcgccag tctgggcacc gaaaaagtgc gcctgacagg
    11221 aggagagccg tctttacgcc gtgactttac cgatatcatc gccgctgtgc gggaaaacga
    11281 cgctatccgc cagattgcgg tcaccaccaa tggttaccgt ctggaacgcg atgtggcgaa
    11341 ctggcgcgat gcgggactta ctggcattaa cgtcagtgtc gacagtttgg acgcccgcca
    11401 gtttcacgct attaccgggc aggataaatt caaccaggtc atggcaggga ttgatgctgc
    11461 atttgaggcc ggttttgaga aggtcaaagt caataccgtg ctgatgcgtg atgttaatca
    11521 tcaccagctc gacacctttc tgaactggat ccagcatcgc cctatccagc tgcgtttcat
    11581 cgaactgatg gaaacgggcg agggcagcga gctcttccgt aagcatcaca tctctggtca
    11641 ggttctgcgt gacgagctac tgcgtcgcgg ctggatccac caattacgtc aacgcagcga
    11701 cggtcccgcg caagtctttt gccatccaga ttacgccgga gagattggcc ttatcatgcc
    11761 gtatgaaaaa gacttctgcg ccacttgcaa ccgcctgcgc gtttcctcca ttggtaaact
    11821 ccatctctgc ctgtttggtg aaggcggcgt taacctgcgc gatctgctgg aaggcgatac
    11881 ccagcaacag gcgctggaag cgcgtatttc agcggcgctg cgggagaaga aacagaccca
    11941 tttcctgcat caaaacaaca ccggtattac gcaaaactta tcgtacattg gcggctaaaa
    12001 cgtcaaaagg agagatcaga tgagtcaggt aagcactgaa tttatcccga cccgtattgc
    12061 tattcttacg gtttctaatc gtcgcggtga agaagacgat acctccggtc actatctgcg
    12121 cgattcggcg caagaagcgg gccatcacgt tgtcgatgaa gccattgtga aagaaaaccg
    12181 ctacgctatt cgcgctcagg tatctgcgtg gatcgccagc gacgatgtac aagtggtatt
    12241 gattacgggt ggtactggcc tgacggaagg tgatcaggct cccgaagcgc tgctgccgtt
    12301 gttcgaccgt gaggttgaag gttttggtga agtgttccgt atgttgtcgt ttgaagagat
    12361 tggcacttcc acgttgcaat ctcgtgcggt agcgggcgtc gctaacaaaa cgctgatttt
    12421 cgccatgccg ggttcgacca aagcgtgccg taccgcatgg gaaaatatca tcgcgccgca
    12481 gctggatgcc cgtacgcgtc cgtgtaattt ccatccacat ttgaagaaat aagtatgtcg
    12541 caactgaccc atatcaacgc cgctggcgaa gcgcacatgg tggatgtctc cgccaaagcg
    12601 gaaaccgtgc gtgaagcgcg ggcggaagcc tttgtcacca tgcgcagcga gacgctggcg
    12661 atgattattg atggtcgcca ccacaaaggc gacgtatttg ccactgcgcg tattgccggt
    12721 attcaggcgg caaaacgcac ttgggatctg atcccgctgt gtcacccgct gatgctcagc
    12781 aaagttgaag tcaatttaca ggccgagccg gagcacaatc gggtgcgtat agaaacctta
    12841 tgccgcctga ccgggaaaac cggtgtcgaa atggaagcgt taaccgcggc ctccgtggcg
    12901 gcgctgacca tttatgacat gtgcaaagcg gtgcaaaaag atatggtgat tggtccggta
    12961 cgtttgctgg cgaagagcgg cggcaagtcg ggtgacttta aggtggaagc ggatgattaa
    13021 agttcttttt ttcgcccagg tgcgcgagtt ggtgggaaca gatgcaaccg aagtggctgc
    13081 ggattaccca accgttgaag cgttacgcca gcacctggct gcgcagagcg atcgctgggc
    13141 gctggcgctg gaagatggca aattgctggc tgccgtcaac cagacgctgg tgagttttga
    13201 ccatccgctg actgacggcg acgaagtagc tttcttcccg ccggtaaccg gaggttaaga
    13261 tggcagaaac caaaattgtt gtcggcccgc agccgttcag cgtaggagaa gagtacccgt
    13321 ggctggcgga gcgtgacgaa gacggtgcgg tagtcacctt caccggaaag gtgcgcaacc
    13381 ataacctggg cgacagcgtc aaagcattaa ccctcgaaca ctatccgggg atgactgaaa
    13441 aagcactggc agaaattgtt gatgaagcgc gtaaccgctg gccgctgggg cgcgtcacgg
    13501 tgattcaccg catcggggaa ttatggccgg gcgatgaaat cgtttttgtc ggtgtcacca
    13561 gtgcgcatcg cagcagtgcg tttgaagccg ggcagtttat tatggattat ctcaaaaccc
    13621 gcgcaccgtt ctggaagcgc gaagccacgc cggaaggcga ccgctgggtt gaagcccggg
    13681 agagcgatct gcaggcggca aaacgctggt agttttttgt tagccggata aggcaccgct
    13741 gcgtccggca atttcaccgc tcagtacatg cgtttacgct gcgtcgatgc acagcctcat
    13801 cattttgcag tatccttaag atattcctta tatcttcagg agatcgtcat ggacagattc
    13861 ccacgttctg attcaatcgt acaaccctgg gctggcttgc aaacttatat ggcgcaagtc
    13921 tatggctgga tgaccgttgg cttgttgctg accgcatttg ttgcctggta tgcggctaat
    13981 tccgcggccg tgatggagct gttgttcact aaccgtgtct ttttaatcgg tctgatcatc
    14041 gcgcaattag cattggttat tgtgttatca gcgatgattc aaaagctgag cgcaggtgta
    14101 acgacgatgc tctttatgct ttattcggcg ctgacgggtc ttacgctttc cagtatattc
    14161 attgtctata ccgctgcttc tatcgccagt actttcgtcg ttactgccgg gatgttcggc
    14221 gcaatgagcc tgtacggtta caccacgaag cgcgatttaa gtggctttgg caatatgctg
    14281 tttatggcgt taatcggcat tgtgctggca tcgctggtca acttctggct gaaaagcgaa
    14341 gcattgatgt gggcagttac ctacatcggc gtgattgtct ttgtcggatt gacggcgtat
    14401 gacactcaga aattgaaaaa tatgggtgag cagattgata cccgcgacac gtcgaacctg
    14461 cgcaaatatt ccattcttgg tgcgttaacc ttgtatctgg acttcatcaa cctgttcctg
    14521 atgttgttac ggatcttcgg caaccgccgt taattctttt tcaccatcac tatcccggcc
    14581 atgtgtcggg atttttttct tactaaaaac gacacttaac aagtaccagg tataaaatat
    14641 gccttatgag cgagcggaat cgttttccgt atccataaat acaaaagtat tttgtatgcg
    14701 tggttttaaa tttattgatg gtgaatatta atgttttttc taaggacaac taaaagaatg
    14761 gaatcatact cgcaaaacag caataaatta gattttcagc acgaggccag gatattaaac
    14821 ggtatatggc tcattaccgc tttaggtttg gtggcaaccg caggactagc ctggggagct
    14881 aagtatctcg aaattacggc aaccaaatat gattcaccac caatgtatgt cgccataggg
    14941 ttattattac tttgtatgta tggcttaagt aaggatatca acaagataaa tgccgccatc
    15001 gcgggcgtaa tatatctgtt tttactctct ttggtggcga ttgtcgttgc aagtttagtt
    15061 cctgtatatg ccattatcat cgtgttcagc actgcgggcg cgatgttttt aatcagtatg
    15121 ctggccggtt tattatttaa tgttgatcct ggttctcacc gttttatcat tatgatgacg
    15181 ttgacagggt tggccctggt aatcatcgtg aatgcggcat taatgagtga acggcccatt
    15241 tgggtaataa gttgcttaat gattgtgtta tggccaggca ttatctcgca tggacgaaat
    15301 aagctccttg aactggcagg aaaatgccat agtgaagagt tgtggagtcc ggttcgttgc
    15361 gcttttacag gtgcattaac actctattac tattttatcg gcttctttgg gatacttgcc
    15421 gcgatagcta taacgcttgt ctggcaaagg catacgcgtt tttttcatta gtcgtaaatg
    15481 cagatactaa agaacttaac taaacttcac atcgccgctt catttttcgc ccgtagcttc
    15541 ttcgcttggc tttccagtag caggtaacag atcagcgcca gcagcagcgg gataaagtaa
    15601 tacaggacgc gataagcgag cagggcggcg ataatgctcc ctttcgaggt atgctcccca
    15661 gccagtagtg cgataaacac cgcttccagc acaccgatcc ccgccggaat atgcacgatg
    15721 acgccagcaa tactactaac cagtaacacg cccagtacaa agaaatagtt cacgctttga
    15781 ccaagtaaca gccagataat cgcccccatt accatccagt taacgctgga aatcagcatc
    15841 tgagcgaggg cgaatttcca tgaaggcagc accagctttt gccctttgat ggtcatatgg
    15901 cggtgcttcg cgaaagcgca aaaccacaaa taaaccgcga taatcatcag taagccaatg
    15961 ccgagaatac gcagcgtagt ttgatcgaca taccagtaat ccggcaactc caccacgcct
    16021 gcggtaaaga taatccctgc cagtaaaata tagcccagcc agttggtggt aatactgagc
    16081 gaggaaatcc gcgtaatagt gctgcccggt aaccctagtc gggagtacaa acgatagcgc
    16141 ataccaatgc cgccgaccca ggtactgagc gtcaggttga aggcgtagca gataaacgac
    16201 accagcatca cctggcgctt cgccagtttg tgaccgcagt aaaagcgggc gagcaggtca
    16261 tagcagccgt aaatcagata gctgacgacc accagcccga ccgcactaag cagcgcaacg
    16321 cgattgtagt cgcggatgac cttccagacc tcttcccagt ccactttttt ggcgtagacc
    16381 accagcaaca caatcaccgc gataaaaaac agccaggtga ggatcttctt tgctaagcgc
    16441 cagcgcgggt gtgatttact catcaggggt ttacccccgt gttttcagtt tctacccgat
    16501 cctgcgtttc cattgtcggt tgtgcggggg gatcaacctg cgtcagacgt ggtgtgtgtg
    16561 ccggaagcca gccaaccagc gccgggaagt ggcgtaaaaa gtggaacgcc agcacgcttt
    16621 tggtcaggtt ccaccaggtg cgtttgggca gcatggtttc atccacctgc tgacaatctg
    16681 cggcaataat gccgttcaga ttatcgcgca gcgtctggtt aaaatgacga tcgtggatga
    16741 tgacatttgc ttcgagattc agtgacaaac tgagcggatc gagattactg gaccctactg
    16801 tcgcccagtg atcgtccatc aatgccactt tgccgtggag cgggcggcgg cggtactcaa
    16861 aaacctgaac gccgccttta accagatagt tatacagcaa gcgcgcaccg actctgacaa
    16921 tcggcatatc cggttcgccc tgaatgatca gtttgatccg caccccgcgc cgtgccgctt
    16981 tacgcaaggc gtgtaaaaat cgatagccgg ggaagaagta ggcgttggcg ataatcactt
    17041 cccgctgcgc ctgagtgagc attttcagat aatggcgttc aatatcatcg cgatgttctt
    17101 cgttatcgcg ccagaccagc aatacctgcg cttctcccgg ctggcggttc tcttccgctt
    17161 tgtgatgacg tcgccaccag cgtcgtgccg cgctctgtcc aggcaggttt tccagctcaa
    17221 actggagaat atcttcgaca atcggccctt caaggcgtac cgcgtaatcc tgtttagcct
    17281 ctggaccgta gctggacata tgctcggcgg agtaattcag cccgccaata aaggcgatac
    17341 gcgcgtcgat caccacaatt ttgcgatgca tccggcgaaa cacattggtg cgcataccaa
    17401 aaaggcgagg gcggggatcg tagtagcgga acactacgcc agctgccgtc agttcattga
    17461 caaactcatc gctgagatcc ggcgaaccgt agccatccag caagacttcc gctttaaccc
    17521 cgcgttgcgc tgctgccagt agtgccgcat gcagctgttt gccgacgtta tcctcaaacc
    17581 agataaacgt ttcaagaatg atgcgttctt gtgcctcgcc aatcgcctta aacaccgcgg
    17641 gataatattg ctcgccgttt tccagcaact ggatcttatt gccttcgcgc cagctacatt
    17701 tcataaatga atctccgcac ttaaaggggc atgatcagaa aggtgtcgcc atgtccgcag
    17761 cggcaacgcg gttggcgcgc tggcgctggc atttttgacg tagatcctgt ccagtcgtag
    17821 tagaggaaat tgcaccggaa acgtgcgcgc cgggcgtccg tgggcgcggg taaaaatctc
    17881 atccagtccg gcctgcactt ttaacggatg attagctttt tgccgccagt cattgaaatc
    17941 acccgccacc aataccggtt cgccgtccgg tagctcattc acccattcgg cgagcatcgc
    18001 aagctgcgcc tgacggtgcg cctcacgcag gcccagatgt acgcacatca catgaatcgc
    18061 ttttccggtc atcggcggca caatgcggca gtagagcacg ccgcgctttt ccgcaccatc
    18121 gaccgaaaca tcgcgattct cataatgttc aatgggataa cgcgacagta cggcgttgcc
    18181 gtgatgccct tccgggtata cggcattgcg accgtaggca aaatcgctcc acatagtgtc
    18241 ggcgagaaac tcgtagtgcg aggtatcggg ccagttttcc acatgcagcg gatgaacttc
    18301 gtgcgcgccc atcacttcct gcaggcaaac aatatcggcg ctgacggtac gcacggcatc
    18361 gcgaagttcc ggcaaaatga agcgtcggtt aaacgcggta aagcctttgt gaatgttgat
    18421 ggtgagcacc ttgaacgaaa attgttgtgt ttgatcgggc ataattttcc tgtctttgcc
    18481 tcttatctca ttgaaatagt gtagtcggcg tcacaaaaag gtgcggtctt acggaatttt
    18541 ccgtaaagtt cggtactctg agtaagtaga gataaattct tcaggagaga agccatgaag
    18601 tggcaacaac gtgttcgtgt cgcaacgggt ctaagttgct ggcagattat gttgcattta
    18661 ctggtagtgg cgctgctggt ggtgggctgg atgagtaaga ctctggttca cgtcggcgtg
    18721 ggattatgcg cactgtattg tgtcacggta gtgatgatgc tggtgtttca gcgccacccc
    18781 gagcaacgct ggcgtgaggt ggcagacgtg ctggaagagc tgaccacgac ctggtatttt
    18841 ggcgcagcgc tgattgtgct gtggctgttg tcccgcgtac tggaaaacaa ctttttgctg
    18901 gcaattgcag ggctggcgat ccttgctggc ccggcggtag tgtctttgct ggcgaaagat
    18961 aagaagttac atcaccttac gtctaaacat cgcgtacgcc gctgaccctg tcgtggccgt
    19021 tatcaccagt agcggccaca aactattcca cacaatatcc agactcgcat ccttcaaata
    19081 aatctgcttg gtaatgtccg taaagtggcg aatagggtta atccacgtca gattttgcag
    19141 ccacactggc atgttttcca ccggcgaaac gtaaccggaa aggagaatgg cgggcatcat
    19201 aaaaacaaac acgccgataa acgcctgctg ttgtgtcgag cagagtgatg aaatcaacag
    19261 accgaatccc accagcgata aaccgtaaat caccatcgta aagtagaaca gcgctagcga
    19321 tccggcgaag gggatttgat acgcccagat accaatcgcc agcacaatgg tggcctggaa
    19381 ggtggcgaca attaacgccg gtacggcttt gccgatgaag atctgccagg tggtgagcgg
    19441 cgaaaccagt agctgatcga gcgtaccttg ttcacgttcg cgggcgacgg aaagtgaagt
    19501 gacgatcatt acgccgatag tggtgatcat ggcgatcagc gacggcacca caaaccattt
    19561 gtagtcgaga ttcgggttat accagttgcg taccaccagc tcgctgttgt tgggtttcgg
    19621 ttttccttcc agcagctcct gctgataatt tttgacgatc tgttgcaggt agttggcggc
    19681 aatttgcgca ctgttggagt tacgcccgtc gaggatcaac tgcaacggcg cggtctggaa
    19741 ggtatccagt ttgcgcgaga agtcagccgg gaaacgcacc agtagtaacg ccttttgtgt
    19801 gtcgatggtc gggcggatct cctgtgggct tttcagcagc agcacatgtg taaaggcgct
    19861 ggcgcgggca aaacgttgag taagttccac cgaatgctcg ccgttatctt catcgtagat
    19921 ggcgatggtg gcgttagtca cttccagcgt ggcggcgaac gggaacagga tcacctgaat
    19981 tagcacgggt aaaatcagaa tcgcgcgggt ttgtggttcg cgcagcaacg actgcaactc
    20041 tttgcggatt aacgtccata agcgatgaaa catgctcttc tccctaatcc agccgacgtt
    20101 tggttttcag ccacgtcagg ccgataaaca tcaccgccga agcgatcaaa aacagcacgt
    20161 ttaccaccag caccactgga atattcccgg cgaggaacag gctttgcagg gtgctgacga
    20221 aataacgagc gggaataatg tacgtcaccg cgcggatcac cgcgggcata ctgtcgatct
    20281 gaaaaataaa gccggaaagc ataatcgacg gcagaaaagc ggcgttcagg gcgacctgag
    20341 cggcattgaa ctggttgcgg gtaatcgtgg aaatcagcag ccccattccc agggtactga
    20401 gtaaaaacag gctggagata aaaaacagaa tcagcagcga accgcgatac ggcacgccga
    20461 gaataaacac tgacaccagc atacacagca acatcgccag catcccgaga aagtaataag
    20521 ggatcagctt acacagcagc agttccgtgc gggtaatctc cgtagagagc agagcctcca
    20581 tggtgccgcg ttcccattct cgcgccacca ccagcgaggt gagaatcgcg ccgatgaccg
    20641 tcatgataat ggtcaccgca cccgggataa taaagtgctg gcttatcgcc gccgggttaa
    20701 accagtagcg ggtttgtaca tcaataagcg gttcaaaagt ctgcccgttg tcctccgctc
    20761 gctgcatttg ccagatctgc cagatccctt cgacataccc ctgcacaaag ttagcggtat
    20821 tcggctcact gccgtcggtg atcacctgaa tcggtgcggt ggcgttggcg cgctccatct
    20881 gttcggcgaa atccacggga ataaccacca gaccgcgaat tttccccgac tgcattttgg
    20941 cgatcagttc ctgacggtta tcgctgatgg tggcgtcgat gtagggcgaa ccggtcatgg
    21001 tgtgggtgaa atccagcgcc gcttcgctac gctgttccag caaaatcccg acccgcagct
    21061 tgctggagtc gaggttaatg ccgtaaccaa aaataaacag cagtagcagt gggatcacta
    21121 ccgcaatcag ccagctactc ggatcgcgaa cgatctgccg cgtctctttg acgcacagcg
    21181 cccgcacgcg atgccaggaa aggatcgggt tactcattgc tatgctcctt atcccagtcg
    21241 tggatcaact gaataaaggc ttgctccatg gtgggatcgg gttgctcatc gttagctgac
    21301 tgtgctttca aatcgtccgg cgtaccgctg gcgattaatt tcccacggta caccaggccg
    21361 atgcggtcgc aatattccgc ttcatccata aagtgggtgg tgaccatcac cgtgacgcct
    21421 ttctctacca tgctgttgat gtgcagccaa aattcacggc gggtgagggg gtcaacgccg
    21481 gaagtcggtt cgtcgagaaa gagaatatcc ggttcatgca tcagcgaaca ggccagcgcc
    21541 agccgctgtt taaaacctaa tggcagttca tcggtggcgt gtgaggcaat acttttcagg
    21601 ccaaatgcct cgctcatacg ggagattttt tcgttctgcg cccgaccgcg taagccatac
    21661 acaccagaga aaaagcgtaa attctgttcg accgtcaggt tgccgtagag cgaaaatttt
    21721 tgcgccatat agccgagatg ctggcgcgct ttaccggaac tctctttcag atccatcccc
    21781 agcaccagcg cctggccgga agtcggcacc agcaaaccgc acatcatctt aaaggtggtc
    21841 gatttacccg cgccgtttgg ccccagcaaa ccaaaaatct ccccacgttt aacggcaaag
    21901 ttgacgtgat cggtggcggc aaaatcacca aatttcttgg tcagttcttt cgcttcgatc
    21961 accgtctcgc cgggtgtgcc ttctaccgta tgcaatattg cacccagcgg cgattctgag
    22021 gttccggcac cgcccagcaa atcaataaac gcatcttcaa aacgcggcgt agtttcgttg
    22081 atgttgattt ccggcatccc gtcggcatgg cgaatatcgt ctggtgtggc ctcttttttg
    22141 aggatcagac gtaccgattt cccctgaatc atgccatcgc tgacctgcgg cagtttcaag
    22201 gcgcgttgca acagtttgcg gttgccctcg tgtggactgg tcatcagaaa gctgcgtccg
    22261 gccatggttt gtgtcagggc ttttggttct ccctgataca gcaactcgcc ttcgttcatc
    22321 agtaacacgt cacggcactg ctcggcttcg tcgagatacg aggtactcca gaggattaac
    22381 atcccttcgc ccgccagctc atgcaccatc tgccacagtt cgcgccgtga gatagggtca
    22441 acgccgacgc cgggttcatc gagcagcaac actttcggtt cgcccaccag ggtacaggcc
    22501 aggccgagtt tttgtttcat cccaccggag agcttgcccg ccaggcgtcc ggtaaacggc
    22561 ccaagagacg taaactccag caggcgagca aaagtttgct tacgtgcctc gccggtgacg
    22621 ctgcgcaaat ccgcgtacag attgaggttc tccatcaccg tgagatcttc atacagacca
    22681 aatttctgcg gcatatagcc gagcacggcg tgcagcgcgc cgtcgttttt gatcggatca
    22741 aagccaatca ccgtggcact gccgctgtcg ggtttcagta atcccgccaa catccgcatc
    22801 agtgtggttt tacctgcacc gtccggcccc accaaccccg tcacataacc ggcgtgaatg
    22861 gtacaatcga gcggcgcgac ggcgggcttg tccatgcccg gaaagcgttt ttccaggccg
    22921 ttcagcgtga taacggcatc attcatgtcc tgcctcgtca ccgaattgta ccgtcactgg
    22981 cattccctgg cgtaacgcat catcggcgtc ggtcaccaca atacgcaggc gatagacgag
    23041 gtcggtacgc agatccggtg tttcgacggt tttcggggta aattcagcag tcggcgaaac
    23101 gaaaccaatc tgcccgtgat acggcttgtc cgggcgacca tcggtataaa gcagcacttt
    23161 gcgccccggc tgggcctggt caagattccg ttcatcaaca taagcgcgca cccacaccgg
    23221 acgcgttagt gaaacggtaa acaccgtgcc accttcattg agaaccgtgc ctggctctac
    23281 cgcgcgcgtt aacagcgtgc catcagacgg tgctatcaac gttgagtcct gtaaattcag
    23341 ctccgcctgc gccagttgcg cctgcgcctg ttcgaggctg gctttcgcct gagcgatgtc
    23401 ctgttcacgg ttaccggaac ggtactgacg caatttatcc tgtgctgatt tcagcgttgc
    23461 ctgtgcctgg tcgcgcgagg agcgggcatt ttccaggtca tttgccgaaa tggtgcggct
    23521 tttccacagt ccttgctggc ggttatagaa gttctgcgca tagtcatagg cggcttgcgc
    23581 ctgtttcacc gctgcggcgg cctgagcgat ttcttcatcg cgatacccgg caagcatcag
    23641 atcatactgc gcctgtgcca ccgagacgcc cgctttcgcc tgcatcaggg caatctcata
    23701 cggcttgtga tccagttcgc ccagcacctg gcccgctttg atagcatcac cttcgtccac
    23761 cgccagcgat tcaacgcgcc ccccaacacg gaaactaaga ttcaccgtac gaatatccac
    23821 gttgccatac agcgtcaggc cgttatcctg gcggctttga taccaccagt agcctccggc
    23881 aaccacggcg gcaagtacca ctaccgccag tccgatcacg acaggttttt tcatcactac
    23941 aaactccttt gcgataatcc ttgcaaaatc aggtcgatat gacaggtcac cgtctggttg
    24001 atcagttcgg tcttttcttc atcgaacgcg gtccagccgg tacgtaacag aattgtttct
    24061 ttgccaagac ggaacgccag aatctcacca atcaacgcat gggtatgaag gatcatgcgg
    24121 gtgtcattag cgtcgcagcc ggtccaggcg gcaatcaggc gtgtcaggtg gctgtgtagc
    24181 ggactaatca cctgttcgtg caccaggtgg taggctgccg tgggagagag ctgctcacgg
    24241 gagataaact tgctgaggtt gacggtgtca tcctgggtga gcagcttaat catgttcctg
    24301 caggcgcgaa ggatcagttc acggatggca gcccgatcag gctgtggttg ggcgaacaag
    24361 cgttcggctt cctcggcatg cggacggaac tgctcgccaa taaaatcggc aatccactgg
    24421 gcgcaggcga ggtacaaatc ttctttcgaa ccgaagtagt aggtgatggc agcgatattc
    24481 tgcccggcct gggcggctat ctcgcgggtg gtggcgttca ttccatattc accaaactgc
    24541 gccagtgcgg cagcaatcag ctgttttttc gcctgttcac ccttgattgt catggcagga
    24601 ttattcatcg cacagtccat tcttaatcaa atgattgatt aagattatga ctccatatgg
    24661 gagttgtcca gtatggctaa gaattttagc tacgccagtc acagggataa tttatgcgct
    24721 gcgtcacaaa aactgctaca ctccgctccc tcatgacatt gtggtttttg tcattttcct
    24781 tttcagtatc tccctgaaaa ctacaccggt aacggtcggg gcggttcgga gtagttatgt
    24841 ctttcgattc tttgggttta agccctgata tcctgcgcgc cgttgccgag cagggttacc
    24901 gtgaacccac ccctattcag cagcaggcga tccctgctgt gctggaaggc cgcgacctga
    24961 tggctagcgc ccagaccggc accggcaaaa cagcgggctt tacgctgccg ctgttgcaac
    25021 acctgatcac tcgccagccg cacgccaaag ggcgtcgtcc ggtacgtgcg ctcattctta
    25081 ccccgacccg tgaactggcg gcacagattg gcgaaaacgt ccgtgattac agcaaatacc
    25141 tgaacattcg ttcgctggtg gtgtttggcg gtgtcagcat taacccacaa atgatgaagt
    25201 tgcgcggcgg cgttgatgtg ctggtggcaa cgccgggacg tttgctggac ctggaacatc
    25261 aaaatgcagt gaagctggat caggttgaaa tcctcgtcct cgatgaagct gaccgcatgc
    25321 tcgacatggg ctttatccac gatatccgtc gcgtgttaac aaaactgcca gcgaaacgcc
    25381 agaacctgct gttctctgcg accttctctg acgatattaa agccctggcg gaaaaactgt
    25441 tgcacaaccc gctggaaatc gaagtggcac gccgcaatac cgcgtctgat caggtgaccc
    25501 agcacgttca ctttgtcgat aagaaacgca aacgcgaatt gctgtcgcac atgattggta
    25561 aagggaactg gcagcaggtg ctggtgttta cccgtaccaa acacggcgct aaccatctgg
    25621 ctgagcagct caataaagat ggcatccgta gtgtggcgat ccacgggaat aaatcgcaag
    25681 gtgcgcgtac ccgtgcgctg gctgatttta aatcgggtga cattcgcgta ctggtggcga
    25741 ccgatatcgc tgcgcgcggt ctggatatcg aagagttgcc gcacgtcgtc aactatgaac
    25801 tgccaaacgt accggaagat tatgtccacc gtatcgggcg taccggtcgt gcggctgcta
    25861 ccggtgaagc gttgtcgctg gtgtgtgttg atgaacacaa actgctgcgt gatatcgaaa
    25921 aactgctgaa aaaagagatc ccgcgcattg cgattccggg ctatgagccg gacccgtcaa
    25981 tcaaagccga accgatccag aacggtcgcc agcaacgtgg cggcatcagg ctgatgaaca
    26041 aacgcaaaac tgcctgatgc gctacgctta tcaggcctac gtgaactctg caatatattg
    26101 aatttgcatg cttttgtagg ccggataagg cgtttacgcc gcatccggca tttcacaaca
    26161 agcacttgtc agcaatttga gaacacggga aaaaatttat ctgttttacc gcccataagc
    26221 caccgagacg ttacttctct atagccaatt gttcgcgcaa ctccattaaa aggtagccca
    26281 gtcgattctt gcccttacca ttaccaccgt ctccccagta agcatcgttt tccgtatgct
    26341 caaccagttt tgcgggcgcg gttgccagca agagcacacg cagttctgca tgctgttcga
    26401 atttagcgcg aagtgcttta cgcatcacct gttctttaac cgactcccag tttttacgaa
    26461 gtggcttgga acgattacgc cccatgcgag cggcgaccat gggggaagaa acccgacgga
    26521 tctcttcgcg gtatttttca tcaaggaatt tttgtgcctg aaaatagtgt tcggaggtgg
    26581 gccaggtatt cccgtcaacc ttgatgggcc tggcggcaaa gttagaaaaa tcaccgtagt
    26641 catcgctggt gctgtagaaa tttatgatgg tgtcttgcat gacgtgttgg atcctttgtg
    26701 ctcgaacggg cattaaaccg cattatgttg gtggttattg cgagccgctt tccagaaaca
    26761 gaaaaaccat tacccctgaa aaccgaaaaa tgccacaata ttggctgttt atacagtatt
    26821 tcaggttttc tcatggcatt aaccgccgcg cttaaagcgc aaattgccgc ctggtataag
    26881 gcgcttcagg aacagatccc cgactttatt ccccgtgcgc cgcagcggca gatgattgcg
    26941 gacgtcgcca aaacgctggc cggagaagaa gggcggcatc tggcgattga agcccccacc
    27001 ggcgttggga aaacgctctc ctatttgatc cccggcatcg ccattgcccg cgaagagcaa
    27061 aaaacgctgg tggtgagtac cgccaacgtg gcgttgcagg atcagattta cagcaaagat
    27121 ttaccgctgc tgaaaaagat cattcccgat cttaaattca ctgccgcttt cgggcgtggg
    27181 cgctacgttt gtccacgtaa tctgacggcg ctcgccagca ctgaacccac gcaacaggat
    27241 ctgctggcgt ttcttgacga cgaactgacg ccaaataatc aggaagagca aaaacgttgt
    27301 gcgaagctga agggcgatct cgacacttat aaatgggatg gtctgcgcga tcatacggat
    27361 atcgctatag atgatgatct ctggcgtcgt ttgagtaccg acaaagccag ctgcctcaac
    27421 cgtaactgtt actactatcg tgaatgcccg ttttttgtta ctcgtcggga gattcaggaa
    27481 gcggaagtgg tggtggcaaa ccatgcgctg gtgatggcgg cgatggaaag cgaagccgta
    27541 ctgcctgacc cgaaaaatct gctgctggtg ctggacgaag gtcatcacct gccagatgtg
    27601 gcccgggatg cgctggagat gagcgctgaa atcaccgtgc cctggtatcg actacagctg
    27661 gacttgttca cgaaactggt cgctacctgc atggagcagt ttcgcccgaa gaccatccca
    27721 ccgctggcga tccctgaacg tttgaatgcg cattgtgaag agttgtatga gcttatcgcc
    27781 tcgttaaaca acattctcaa tctctacatg cctgccgggc aggaggcaga gcaccgtttt
    27841 gcgatgggcg aactgcctga tgaattgctg gagatctgcc agcggctggc aaaactcacc
    27901 gagatgctgc gtggtctggc ggagttattt cttaacgatt taagtgagaa aaccggcagc
    27961 cacgatattg tgcgtctgca tcggttgatt ttacagatga accgcgcgct ggggatgttt
    28021 gaggtgcaaa gcaaactctg gcggctggcg tctctggcgc aatcttccgg tgcgccggtg
    28081 accaaatggg cgacgcggga agagcgcgaa gggcagctac acctctggtt tcactgcgtg
    28141 ggaatacgtg tcagcgatca gctggaaagg ctgctgtggc gcagtattcc gcacattatt
    28201 gtcacctccg caaccttgcg ttcgctgaat agtttttcgc gtttgcagga gatgagtggt
    28261 ctgaaagaga aagcgggcga ccgttttgtg gcgctggatt ccccctttaa ccactgcgaa
    28321 cagggcaaaa ttgttattcc ccggatgcgc tttgagcctt ccatcgacaa cgaagagcaa
    28381 cacattgccg aaatggcggc ctttttccgt gaacaggtgg agagcaaaaa atatctcggt
    28441 atgttggtac tgtttgccag cggacgggcg atgcagcgct ttctcgacta tgtgacggat
    28501 ttacgcctga tgttgctggt gcagggcgat cagccgcgtt atcgcttagt tgaactgcac
    28561 cgcaaacgcg ttgccaacgg tgagcgtagc gtgctggtgg gcttacagtc atttgccgaa
    28621 gggcttgatt tgaaaggtga tctgctcagc caggtgcata tccacaaaat cgcttttccg
    28681 cccatcgaca gcccggtggt gatcaccgaa ggggaatggc tgaaaagcct caaccgctat
    28741 ccgtttgagg tgcaaagcct gccgagcgcc tcgtttaacc tgattcagca ggttgggcga
    28801 ctgattcgaa gccacggttg ctggggcgaa gtggttatct acgacaaacg cttgctgaca
    28861 aaaaattatg gcaagcgact actggatgca ttaccggtat ttccgataga gcaaccggaa
    28921 gtccctgaag gtatagttaa aaagaaagaa aaaacgaaat ccccacgccg tcggcggcgt
    28981 taatgatgtg agtcaggtaa ggagtcgtaa atggactatc gcaaaatcat taaagagatc
    29041 gggcgcggga aaaaccacgc gcgcgattta gaccgggata ctgcgcgcgg tctgtatgct
    29101 catatgctca atggtgaagt ccctgacctt gagttgggcg gcgtattgat tgcgctgcgt
    29161 atcaaagggg aaggggaagc agagatgctc ggcttttacg aagccatgca aaatcacacc
    29221 atcaagctga cgccgcctgc aggcaagccg atgccgattg tcatccccag ttacaacggc
    29281 gcgcgtaaac aggctaacct gacgccatta ttggcgattc tgctgcataa actcggtttt
    29341 cctgtggtgg ttcacggagt tagcgaagat ccaactcgcg tgctgacaga aaccattttc
    29401 gaactgatgg gtattacacc aacgttgcac ggcgggcagg cgcaggcgaa gctcgacgaa
    29461 catcaaccgg tgtttatgcc tgtcggcgcg ttttgcccgc cgctggaaaa acaactggcg
    29521 atgcgctggc ggatgggcgt gcgtaacagt gcacataccc tggcgaaact ggcgacgcca
    29581 tttgccgaag gtgaggcact gtgtctttcc agcgtttcgc atccggaata cattggacgc
    29641 gtcgcgaagt tctttagcga tatcggcggg tgggcgttac taatgcatgg tactgaaggc
    29701 gaagtgtatg ctaatccgca gcgctgcccg cagatcaatc tcattgaccg tgaagggatg
    29761 cgggtgctgt atgaaaaaca ggaccctgct ggtagcgagt tactgccaca agcaaaagat
    29821 ccggaaacca cggcgcagtg gactgagcgt tgcctggctg gcagcgaagc gattcccgaa
    29881 tcgctgaaaa tccagatggc ttgctgcctg gtggctacgg gtgaagcggc aactatcagc
    29941 gatggcctgg cgcgcgttaa tcaggcattt taatttttat ctcagccgga tgagccacgt
    30001 catccggctt ttaccccacc gcaatatgaa attcctgcat ctttattgac cttcccgcgc
    30061 ccggcgtgca gcataaaaaa acaacaaaca cataacataa acaggagtta accatggaaa
    30121 gtggtcatcg ctttgatgct cagacgctgc acagttttat tcaggctgta tttcgtcaga
    30181 tgggtagcga ggaacaagaa gcgaaattag ttgccgatca tttaatcgcg gcaaacctgg
    30241 cagggcatga ttctcatggt attggcatga tcccaagcta tgtgcgttcc tggagtcagg
    30301 ggcatctgca aattaaccat catgccaaaa tcgttaaaga ggcgggggcg gcagtcacgc
    30361 tcgatggcga tcgcgcattc ggtcaggtcg cggcacacga agcgatggcg ctggggattg
    30421 agaaagcgca tcagcacggt attgctgccg tggcgctaca taactcgcat catatcggcc
    30481 gtatcggtta ctgggcggag cagtgttcag cggcggggtt tgtctctatc cactttgtta
    30541 gcgtggtcgg tattccaatg gtcgcgccgt tccacggtcg cgacagccgc tttggcacca
    30601 atcccttctg tgtggttttc cctcgtaaag ataatttccc gctgttgctt gattacgcca
    30661 ccagcgccat tgcatttggc aaaacccgcg tcgcctggca taaaggcgtc cccgtgccgc
    30721 caggttgcct gattgacgtt aacggcgtgc cgacgaccaa tccggcggta atgcaggagt
    30781 cgccgttggg gtcgctgttg acctttgccg aacataaagg ctacgccctt gcagcgatgt
    30841 gtgaaattct tggcggggcg ctttctggcg gtaaaacgac gcatcaggaa acgttgcaaa
    30901 ccagccccga tgccattctt aactgcatga ccactatcat catcaacccg gaactgttcg
    30961 gcgcgccgga ttgtaacgcg cagaccgaag cctttgccga gtgggtgaaa gcctcgccgc
    31021 atgatgacga taagccgatt ttgctaccgg gcgagtggga agtgaacacg cgtcgcgaac
    31081 ggcaggagca ggggattcca ttggatgcgg gaagctggca ggccatttgt gatgcggcgc
    31141 ggcagattgg tatgtcggaa gagacgttgc aggctttctg tcagcagtta gccagctaaa
    31201 aaaaagcccg tccagtggcg gacgggcaaa caagggtaac ataggatcaa tgagggttag
    31261 agcatatgcg tctgtcggca aacagacagg gaaatacttg tgctggacgt agcgtaaacg
    31321 cctgatccgt ccaacggttc ggattttgta ggcctgataa gacgcggtag cgtcgcatca
    31381 ggcatctgtt gccggatgcg gcataaacgc cttatccgtc ctacgaatta cttataaatt
    31441 accgcagtac cgcttaattt attgttgttg gtggcggaag tgatgctgta tccactggca
    31501 ccggctgccg ccgctttctc agccagtttc gcttccaggg catcgagggg ggatgcgcca
    31561 tcggcagaaa ccacgccgat tttattcatg ttctgtgcct gggatgccgt taccggttcc
    31621 gccgcgaaca cgccaaatga cagagttgaa agagccatag cagcaacaac agtattgata
    31681 gttttcataa ttaatctctc gcaggtgatt tttgtataag tgacgttgtt tcgtcgatgt
    31741 gatgagtatc acgttttttt acgagagata aaatcgaaga gaattgaccg ccttgttcaa
    31801 ataaattgat tgatatctaa catactgata aaccatttat ttgttgtact tatggacttg
    31861 ttttatgccg cgtggcagtc acagcagcgt taaaggcacg gaatgccacg caaaatacaa
    31921 attatactcg ctatgcatct cgccagttgt agttaacgta actgtctgtc tttcgaacct
    31981 ctgcgattat atcctgtata cgctggtttt tgtaaatctt tctcctgctg acaatgaata
    32041 cataagcgca cgccaggaat ggcttcccga cgggcttgcg ggatgggggc accgcactct
    32101 tcacattcat acaggctttc gccacgcgga atttcacccc gagcgcgggc aatcgcatct
    32161 tcaattgtac tgttgatctg ttcgttgacg gcgtcatcgt tagcccaagc ggatgccata
    32221 tcgtcctccc catatcaatg cttgtacagt taagtgtagc taatataggg acgaacctgg
    32281 gcagttcaag cgtcagatct ccgaccattc ccgcagcaga ttatgataaa gattgagcag
    32341 cgacaggatc tcttcatttt caccatagcg gcttttcagc gactgaatat ttttgtccag
    32401 ttcaaacagc atggcgcgct ttttatcatc gcggatcatc gactggatcc acataaatga
    32461 tgccactcgt acgccgcggg ttacgggtgt cacgcaatgc aggctgctgg agggatacaa
    32521 caccagatca cctgccggga gttttacccg atgttgtcca aaggtgtcat taacgaccag
    32581 ttcgccgccg tcgtagcttt gtggatcgct taaaaacagc gtggcagaaa ggtcagtgcg
    32641 catccagccg ttttgtggat ggctgcgtac tgcgccatcc acatggaaac cataggtttc
    32701 attgttctga tagcgattaa acagcggcgt ggaaagggta cgcggcaagg cagcggcaaa
    32761 gaacaatgca tgttggttaa ccgcgttcag cacctcattt tgcagtgcgg cgtataacgc
    32821 gctgcgggtg tctacttgct gattgttctt aacttgtgca ccttgtgctc cggtggtgac
    32881 gcgtccatcc acccattcgg cttgttccag ttgttcgcga aaatgggcga cgtcctgtgg
    32941 cgataacacg ccgggaatgt ggtacatcat acaagttctc caaaaagtgg ggcctgcgcc
    33001 ccacatctga atcagaaatg catattggct gtgagcaaga aggttcttgg ctcgcccggg
    33061 tgataacggt agccgctctt gttgattgag gcgacgtaat cggtatcaaa caggttgtaa
    33121 acgtttagct ggaagtcgag attgcgatta acccgatacc ccagtttggc atcggcgacc
    33181 cagtaacctt ctgtaaatgc tggcgttccc accgcgccgt ctgaaccttt atgtatactg
    33241 ccgatatagc gtgcgcccgc gccaacagag atatcatcgg ttgcctgata ttggctccat
    33301 aaggtgaagg cgtgctccgg ggtatacggc agcgatgagg aaccatcctg ggcaacatct
    33361 ttgccgtttt tgatggttgc tttttgctgg gtatagccgc caatcacctg ccacgcggga
    33421 gtgatattcc ctgccacgga tatctcatag ccttcgacgc gtttcttacc gtattgcgag
    33481 taagtcccgt catcattttg ctcaacttca ttttcgatat cagtacggaa cagcgcggcg
    33541 gtgagcaaca ggcgtttatc cagaacctgc catttggtgc caatctcgct ggtgttggct
    33601 ttttgcggtt taaaatcggt gcggttggca ctgttaccac tgccagactg cgcaagggcg
    33661 aagttgttgc cgcccggagg ctgctgggaa acggcatagt taatatagac attgccgttt
    33721 tccgtcaggt ggtacagcgc cccggctttc cagttcacca gattgcccga cttggcggtg
    33781 tcgacggtgg tgactggaga accttttgcc acaccagctg ggcaggtgat ggcaccgcgt
    33841 ccgctgccgc cgcaggcggt ggcactgtca tattcagtat gataattatc cagacggatc
    33901 ccgccgttca gctcaaaatc acgggtgatt tgcagcgtat cgaaggcgta aattgcgaag
    33961 gtatccgtct gaccattggc gtttgcgccg ttgcgcgtca ggccgccggg atgaattctg
    34021 ctgtcaggat gataaatatt taccgccggt aacgtcaccg gattaacgcc atagttagtc
    34081 tgcgtttcac gggtaaattc cacgccggta ctgacatcat gaccgataga accggtatag
    34141 aacgtcgagg tcaggttggt ctggttggtg agaattttat tactcacatc tttggtattc
    34201 gccgtgcgtg actaggtcca gctattcaca tcgctggtgg gctgagtaat attcgacgcc
    34261 ccgcccataa tcgccgtcat caggtaatcc tgctttacgc gcgaccaacg ggtagtattg
    34321 cgaatggtgg tgttatcgtt gatgtcgtgc tcaaaacgca tggtggcggt gtcggtggtc
    34381 gaatcgtcgt aatcggaatc cgtgccgtaa aagttatgag tatcaacttt tccggaatga
    34441 ttcagggccg ccgctcccgc agatggggca gaatagcccg gcaaaccgat ggtcggaatg
    34501 ccgccgtctg gcgtgttgtg ctgagtgaca tgcagataat taagatacaa acgatttgct
    34561 gtaccaaggc caaaagcgac agaaggggcg acgccgtaac gctcattttt gactttgtcg
    34621 cgtccggcat cgtgggtttt ttcgcccatc acattcaggc gcaccgcggt agtatcacca
    34681 atgacctgat tgacgtccag cgtgccgcgg cggaaccagg cgctgccaat actggcggag
    34741 gcgtcaatgc cggaatcatt gcgcggctgc ttgctgatca tattgatcga gcctgtcggt
    34801 gcgctgcgcc cgtagtcggt gccggacggc cctttaatca cttcgacctg ctcggtattg
    34861 aaggtgtcgc gcgagacgct gccgatatcg cgaataccat caatataaat actgttagag
    34921 gtatcggcac cacgcatata aatggcgtcg ccagtggtgg agttaccgtt ctcacccgca
    34981 aaaaacgcac ccacgccgg
//
LOCUS       DAZPCS010000027        34800 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZPCS010000027 DAZPCS010000000
VERSION     DAZPCS010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34800
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.027"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..2098
                     /locus_tag="ACHUQ7_001309"
     CDS             <1..2098
                     /locus_tag="ACHUQ7_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HFR1134798.1"
                     /translation="FNGYDADDVSDSNTDVSDLYYRYDFLRRYYVQAGRMDNRTLFNA
                     QGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGTPVMVLLSRNSRVDAYRNEQLLG
                     SFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRTELVPFTKTGGLTDGNAQWFLQA
                     GKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANADDVSAFELGNNWTADLGRVGNL
                     AISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTNSDGDACTTDSRESYNALSCYES
                     ISATVSLNFVGWNMMLGYTCTQNNTDDSLRWDKQQSFENNYLRQTTAQSISETVQLSA
                     SRAIVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSFSLSDTPTMDSNNNSHSTNVPTD
                     YRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGLNTDTINSAVNGRWDGQYGNVYA
                     TVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGASGTDDLLGAVLVDVKGFSEQDE
                     ESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGFVEVNDSSQGNQQGTTNIINGAG
                     NRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKKFPIVGLNSAMLLVAEDGGFTLE
                     INGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDVTCSVVTYSQLPESIQVQAQLKQ
                     PKLRGNVQTAQREVAP"
     gene            2095..3183
                     /locus_tag="ACHUQ7_001310"
     CDS             2095..3183
                     /locus_tag="ACHUQ7_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CfaE/CblD family pilus tip adhesin"
                     /protein_id="HFR1134799.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPLGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(3271..4569)
                     /gene="exuT"
                     /locus_tag="ACHUQ7_001311"
     CDS             complement(3271..4569)
                     /gene="exuT"
                     /locus_tag="ACHUQ7_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HFR1134800.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            5054..6466
                     /gene="uxaC"
                     /locus_tag="ACHUQ7_001312"
     CDS             5054..6466
                     /gene="uxaC"
                     /locus_tag="ACHUQ7_001312"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFR1134801.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            6481..7968
                     /gene="uxaA"
                     /locus_tag="ACHUQ7_001313"
     CDS             6481..7968
                     /gene="uxaA"
                     /locus_tag="ACHUQ7_001313"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFR1134802.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQLDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            8051..8602
                     /locus_tag="ACHUQ7_001314"
     CDS             8051..8602
                     /locus_tag="ACHUQ7_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFR1134803.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(8607..9851)
                     /gene="sstT"
                     /locus_tag="ACHUQ7_001315"
     CDS             complement(8607..9851)
                     /gene="sstT"
                     /locus_tag="ACHUQ7_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFR1134804.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPFGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(10447..11412)
                     /gene="alx"
                     /locus_tag="ACHUQ7_001316"
     CDS             complement(10447..11412)
                     /gene="alx"
                     /locus_tag="ACHUQ7_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFR1134805.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(11683..12681)
                     /locus_tag="ACHUQ7_001317"
     CDS             complement(11683..12681)
                     /locus_tag="ACHUQ7_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFR1134806.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLGSEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKLYLPK"
     gene            complement(12760..13452)
                     /locus_tag="ACHUQ7_001318"
     CDS             complement(12760..13452)
                     /locus_tag="ACHUQ7_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFR1134807.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRT
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(13529..14032)
                     /locus_tag="ACHUQ7_001319"
     CDS             complement(13529..14032)
                     /locus_tag="ACHUQ7_001319"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFR1134808.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGSKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            14117..15253
                     /gene="rlmG"
                     /locus_tag="ACHUQ7_001320"
     CDS             14117..15253
                     /gene="rlmG"
                     /locus_tag="ACHUQ7_001320"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFR1134809.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     LGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHYARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            15538..15852
                     /gene="higB"
                     /locus_tag="ACHUQ7_001321"
     CDS             15538..15852
                     /gene="higB"
                     /locus_tag="ACHUQ7_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HFR1134810.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            15849..16265
                     /gene="higA"
                     /locus_tag="ACHUQ7_001322"
     CDS             15849..16265
                     /gene="higA"
                     /locus_tag="ACHUQ7_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFR1134811.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(16310..18328)
                     /gene="fadH"
                     /locus_tag="ACHUQ7_001323"
     CDS             complement(16310..18328)
                     /gene="fadH"
                     /locus_tag="ACHUQ7_001323"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFR1134812.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQGCLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(18554..20905)
                     /gene="ygjK"
                     /locus_tag="ACHUQ7_001324"
     CDS             complement(18554..20905)
                     /gene="ygjK"
                     /locus_tag="ACHUQ7_001324"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFR1134813.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATQEQTRVAVKAIETLNGNWRSPGGAVNYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(20922..21993)
                     /gene="ygjJ"
                     /locus_tag="ACHUQ7_001325"
                     /pseudo
     CDS             complement(20922..21993)
                     /gene="ygjJ"
                     /locus_tag="ACHUQ7_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311988.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
     gene            complement(22127..23560)
                     /locus_tag="ACHUQ7_001326"
     CDS             complement(22127..23560)
                     /locus_tag="ACHUQ7_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR1134814.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(23623..24072)
                     /locus_tag="ACHUQ7_001327"
     CDS             complement(23623..24072)
                     /locus_tag="ACHUQ7_001327"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFR1134815.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(24069..27155)
                     /gene="ebgA"
                     /locus_tag="ACHUQ7_001328"
     CDS             complement(24069..27155)
                     /gene="ebgA"
                     /locus_tag="ACHUQ7_001328"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HFR1134816.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SSQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHALTRGELKVENKLWFTTLD
                     DYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSRTR
                     YSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKMSG
                     KPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQSD
                     GEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFTMG
                     INGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTA
                     LTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGSN
                     SWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ"
     gene            complement(27338..28321)
                     /gene="ebgR"
                     /locus_tag="ACHUQ7_001329"
     CDS             complement(27338..28321)
                     /gene="ebgR"
                     /locus_tag="ACHUQ7_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFR1134817.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            28539..28870
                     /locus_tag="ACHUQ7_001330"
                     /pseudo
     CDS             28539..28870
                     /locus_tag="ACHUQ7_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            complement(28912..30291)
                     /gene="ygjG"
                     /locus_tag="ACHUQ7_001331"
     CDS             complement(28912..30291)
                     /gene="ygjG"
                     /locus_tag="ACHUQ7_001331"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFR1134818.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGVFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            30709..32229
                     /gene="aer"
                     /locus_tag="ACHUQ7_001332"
                     /pseudo
     CDS             30709..32229
                     /gene="aer"
                     /locus_tag="ACHUQ7_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
     gene            complement(32383..33006)
                     /gene="nfeR"
                     /locus_tag="ACHUQ7_001333"
                     /gene_synonym="yqjI"
     CDS             complement(32383..33006)
                     /gene="nfeR"
                     /locus_tag="ACHUQ7_001333"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFR1134819.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            33283..34028
                     /gene="nfeF"
                     /locus_tag="ACHUQ7_001334"
                     /gene_synonym="yqjH"
                     /pseudo
     CDS             33283..34028
                     /gene="nfeF"
                     /locus_tag="ACHUQ7_001334"
                     /gene_synonym="yqjH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
     gene            complement(34082..34157)
                     /locus_tag="ACHUQ7_001335"
     tRNA            complement(34082..34157)
                     /locus_tag="ACHUQ7_001335"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34122..34124),aa:Ile,seq:cat)
     gene            34282..34788
                     /gene="mug"
                     /locus_tag="ACHUQ7_001336"
                     /pseudo
     CDS             34282..34788
                     /gene="mug"
                     /locus_tag="ACHUQ7_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
BASE COUNT         8488 a         8948 c         9143 g         8221 t
ORIGIN      
        1 tttcaacggt tatgatgcag atgatgtcag tgacagtaat actgacgtca gcgatctcta
       61 ttatcgttat gattttttac gtcgttatta tgtgcaggcg ggtcgcatgg acaaccgcac
      121 actatttaat gcacaaggcg ggaactttac ctttaacttt ctgccactcg gtgcaatcga
      181 tgggatgcgt atcgggtcga cactgagcta tttaaaccag gcgcaaagcc agcaaggaac
      241 cccagtaatg gttctgcttt cgcgcaattc tcgtgttgac gcttatcgta atgagcaact
      301 tttgggatcg ttttatctca atagtggttc gcaatttatt gataccagtt cctttccgcc
      361 aggcagctac agcgtggcat taaaagtcta cgaaaataac caactcaccc gcaccgagct
      421 tgtgccgttt accaaaaccg gcggtctgac tgacggaaat gcgcaatggt tcttacaggc
      481 aggtaaaact acatcacagg tttctgatga tgaaagttca gcttatcagc taggagtacg
      541 cctgccatta catccgcaat atgagctcta cgcagggctg gcgaatgctg atgatgtgag
      601 tgctttcgag ttaggtaata actggacggc agatttaggc agggtgggga atcttgcaat
      661 cagcgccagc gtgttccgta acgatgacgg cggcaaaggt gatatgcaac aggccaactg
      721 gagtaatccg ggatggccga cgttgggctt ttatcggacc aactctgacg gcgatgcttg
      781 tacaaccgac agcagagaga gctataacgc cttaagctgt tatgaaagta tttccgcgac
      841 agtttcactg aattttgtcg gctggaatat gatgctgggt tatacctgca cacaaaataa
      901 cactgatgat agtttgcgtt gggataaaca gcagagcttt gaaaataact atcttcgcca
      961 gacaacagcg caaagtatct ctgaaactgt acaacttagc gcttcccgcg ctattgtgat
     1021 gcgtgactgg attttgagta cgtcagtcgg tgttttccat cgtaatgaca acggtggcga
     1081 taacgacgac aacggcttgt acttatcgtt ttcgttatct gacacgccaa cgatggatag
     1141 caataacaac agccattcaa ccaatgtgcc tacggattat cgttatagcg aacaggatgg
     1201 cgatcaaacg tcatggcagt tatcgcatac cttttataac gattcattca gccataaaga
     1261 acttggcgta accgtcggag gcctgaacac cgataccata aacagcgcgg ttaacgggcg
     1321 ttgggatggg caatacggaa atgtctacgc taccgtatct gatagttatg accgtaagaa
     1381 tcatgatcat ctttcggcct ttacgggcac ttatagctct acgctggctg tcagtcgcta
     1441 tggcgttaat ttgggagcca gtggtacaga cgatttgctg ggtgcggtac tggtggatgt
     1501 gaaaggcttc tctgaacagg atgaagagag tcaggatctg caactcgaag cgcgggtggc
     1561 aggcagccga actttgcagc ttggtcaaag tgacagtgtg ttgttccctt atcctggatt
     1621 tcagtctggt tttgttgagg ttaacgacag tagccagggc aatcagcagg ggacaacaaa
     1681 catcattaac ggtgcaggga atcgtgaatt aatgttgttg cctggcaagc tgcgctatcg
     1741 cgaagtgtct gccagcttta actacaacta tatcggtcgt ttattactgc cggcagcggt
     1801 gaaaaaattc ccgattgtgg ggctgaacag cgccatgtta ctggtagctg aagatggcgg
     1861 atttacactt gaaattaacg gcagcgaaaa agagctctat ctgctttccg ggcagcaatt
     1921 ccttaagtgt ccactgagtg ttgtaaagaa acgcgccagc attcgttaca gcggagatgt
     1981 tacttgtagt gtggtgactt attcacaatt accggagtcg attcaggttc aggcacagtt
     2041 gaaacagcct aaattacgtg gaaacgttca gacggcgcaa agggaggttg caccatgaga
     2101 aatcgattga ttgcggcgat attgggtttg tttggcacgc tcactggcgt tcaggcagct
     2161 cctgacgtga ccagtgaaat tacgtatgat ttggcatctg gtagagcgga ttattacttc
     2221 tggaaggatg aagcgtcggc aggaaataat ggatatatgt ggtatgaatg ttcgtatcct
     2281 gacctccaac aaacctgtac agctaatgga aatatatcga cagtacaaat ctatttaact
     2341 gaacaacgca gtgggatgcg ttggccggta aaactcaaag gatttaaaac agccattgta
     2401 agtagcgatg aagcgccgct aggatgcaag gggggcaaag ggcttcagac gaatcttaag
     2461 gattctaata gatcttcatg tacagaagat ggtcaacatt attatatata cgatacaaag
     2521 tttcttacgc tctaccttga gcagacagag atgaagaatt tgccgattgg tggcgtctgg
     2581 aaggggaaag ttaaattaca ttcgaacagc ccggcccagg actatttcgc aaatattacc
     2641 ctgaatacgc tcgaccccaa ccatattgac gtgttcttcc cggagttcgc ccacgccacg
     2701 ccaagggtgc agttagactt gcatccaaca ggaagcgtta acggcagcaa ctacgcgcaa
     2761 gatctgacta tgttggacat gtgcctgtac gatggtttta acggtaatgc catcagttat
     2821 gaaatcatgc tcaaagatga agggcgacca gctgcagggc gcagagacgg ttacttctct
     2881 atctatcgtc agggagggac caccaccgac gagggagaac gcattgatta ccgggtcaaa
     2941 atgtacaacc cggaaaccgg tgggcaaatt gatgtgcgca ataatgaaaa tatggtctgg
     3001 aacagcatta acctgaaacg tgtgcgtccg gtggtactgc ccggtattcg ctatgccgtg
     3061 atgtgtgtgc cgacaccctt gactctggca gtcgataaat tcagcgtgat ggataaacag
     3121 gccgggtact acatgggcaa attgtcagta atctttacgc cttccttgcc aaccatcaat
     3181 taaccctgca aaccggacaa acgctttttt ctatataaac gacgaagccg cctgataagg
     3241 cggcttcgtc gttataactt acgttaaacg ttaatgttgc ggtgcgggat cgttatgtgt
     3301 ttcctgcgcc acctcaatcg ccggtttgtt ctgcaatacg gtccagataa ccagcgcacc
     3361 cagcaggtca aatactgcca gaactgcgaa cagcgggctg aagccgatgg tgtcagccag
     3421 tgcaccgaca accagcgcaa acagcgtact tgccagccat gcggacatcc cggttaaacc
     3481 gttcgccgtc gccacttcgt tacgaccgaa cacgtcagaa gagagcgtaa tcagcgcgcc
     3541 agacagtgcc tggtgggcaa aaccaccgat acacagcagc ataattgcga catacgggtt
     3601 ggtgaacaga ccgatcatac ccgggccaat catcagcaca gcacccagcg ttacgaccat
     3661 cttacgggaa acgatcaggt tcacaccaaa ccaacgctgg aacagcggcg gcaggtaacc
     3721 accgaggata caaccgaggt cagcaaacag catcggcatc caggcgaaca tcgcgatctc
     3781 tttcaggtta aagccgtaaa ctttaaacat gaacagcggg atccacgcgt taaaagtacc
     3841 ccaggccggt tctgcaagga aacgcggcag tgcgataccc cagaactgac ggttacgcag
     3901 gatctgacca acggacattt tcttcgccgt gctcacctgg tgctgggctt cctgaccatt
     3961 aataatatag tcgcgttctt catcggtcag atgcttctgg tcgcgcggat gtttatagaa
     4021 aatcagccat gccatcgccc agataaagct caatgcaccg gagatgataa atgccatctg
     4081 ccagctgtgc atcacgattg cccataccac cagcggcggc gcaatcatcg caccaatcga
     4141 agaacctacg ttaaagtagc ctactgcgat ggaacgctct ttcgccggga accattcgga
     4201 gctggctttc agacccgccg ggatcatcgc tgcttccgcg gcaccgaccg caccacgagc
     4261 aacagctagg ccaccccagc tacctgccag cgcggttgca ccacagaaca cggcccacag
     4321 cacagcaaac attgcataac cgattttcgt acccagcaca tccagtacat aacctgctac
     4381 cggttgcatg accgtataag cagcagaata ggctgcgatg atataggaat actgttgggt
     4441 ggagatgttt aactcttcca tcagagttgg cgcagctgcc gccacagtgt tacgcgtcag
     4501 gtaaccaagc acggtgccga gcgtcaccag tgcgatcata taccaacgta accctttaat
     4561 tttacgcatg taaaacctca tcgttttgtt atatccgcag gaagcgcggc catcatcccg
     4621 ccagaggctg aagatgttag ttacgaccgg gtaaccgaaa aaaaaaaccg gcacgccccg
     4681 aacgttgcca tgaaagtttc gtgctaattc ggcttccgta ccggttaacc cgattcatct
     4741 aacaaatgaa tgaatcggca atttgcattc cgtcgactta tgatttgcga cggcagaaag
     4801 ataacttgtc atacaacctt aaaaggtgag agccatcaca aatgtgcgaa tatttgtagg
     4861 gacattacct gacgacagcg aggccagtac tggcgcggcc tgcagcgaga tttaccactt
     4921 tgagagtaat ttttttaact acgtttattg atctaactca cgaaaatatc ttcggactct
     4981 ggaaattggt gtgataactt tgtcagcatc gcaccataag caagctagct cactcgttga
     5041 gaggaagacg aaaatgactc cgtttatgac tgaagatttc ctgttagata ccgaatttgc
     5101 ccgccgtctg tatcacgact acgcaaaaga ccagccgatt ttcgattacc attgccattt
     5161 gccgccgcag cagattgcgg aagactatcg ttttaaaaac ctgtatgaca tctggttgaa
     5221 gggcgatcac tacaaatggc gcgctatgcg taccaacggt gtggctgagc gtctgtgtac
     5281 tggtgatgcg tctgaccgtg aaaaatttga cgcctgggcg gcgactgttc cgcacactat
     5341 cggcaacccg ttataccact ggacgcacct cgaactgcgt cgtccgttcg gtatcactgg
     5401 caaattgctt tctccgtcaa ctgccgatga aatctggaac gaatgtaacg aactgctggc
     5461 gcaggataac ttctccgcgc gcggcatcat gcagcagatg aacgtgaaaa tggtcggcac
     5521 caccgatgac ccgatcgatt ctctggagca tcacgcagag atcgccaaag acggctcttt
     5581 caccatcaaa gtgctgccga gctggcgtcc ggacaaagcc ttcaatatcg aacaggcgac
     5641 ctttaacgac tacatggcga agctgggcga agtttccgat accgacattc gccgctttgc
     5701 tgacctgcaa actgccttga ccaaacgtct ggatcacttc gccgctcacg gctgtaaagt
     5761 gtctgaccat gcgctggacg tggtgatgtt tgctgaagcg aacgaagcgg aactggacag
     5821 cattctggcg cgccgtctgg ctggcgaaac cctgagcgag cacgaagtgg cacagttcaa
     5881 aactgcggta ctggtgttcc ttggtgccga atatgcacgt cgcggctggg tacagcagta
     5941 ccacattggc gcgctgcgta ataacaacct gcgtcagttc aaactgctgg ggccggatgt
     6001 aggctttgac tccatcaacg accgtccgat ggcggaagag ttgtctaagc tgctgagcaa
     6061 gcagaacgaa gaaaacctgc tgccgaaaac cattctctac tgcctgaacc cgcgcgataa
     6121 cgaagtgctg ggcaccatga tcggtaactt ccagggcgaa ggtatgccgg gcaaaatgca
     6181 gttcggttcc ggctggtggt ttaacgatca gaaagacggt atggaacgtc agatgaccca
     6241 actggcgcag ctcggtctgc tgagccgctt tgtcggtatg ctgactgaca gccgtagctt
     6301 cctgtcatac acccgtcacg aatacttccg ccgcattctg tgccagatga tcggtcgctg
     6361 ggtggaagcg ggcgaagcac cggcggacat caacctgctg ggcgagatgg tgaaaaatat
     6421 ttgctttaac aatgcgcgtg actacttcgc cattgaactg aactaaggtc tggggttgat
     6481 atgcaataca tcaagatcca tgcgctggat aacgtcgcgg tcgctttagc agatttggct
     6541 gaaggcacag aagtcagtgt cgataaccag actgttacgc tgcgccagga tgttgctcgt
     6601 ggacataaat ttgcgttaac ggatatcgca aaaggggcca acgtcatcaa atatggcctg
     6661 ccgattggtt atgcattggc ggatattgcg gcgggggaac acgtccacgc ccacaatacg
     6721 cgcacgaatc tgagcgatct ggatcagtat cgctatcaac ttgattttca ggatctgcct
     6781 gcgcaagcgg cagatcctga agtgcagatc tatcgtcgcg ctaacggcga tgtcggggtg
     6841 cgtaacgagc tgtggatcct gccaaccgtg ggctgtgtca acggcatcgc gcggcaaatc
     6901 cagaaccgtt tcctgaaaga gaccaacaac gccgaaggta ccgacggcgt gttcctcttc
     6961 agccacacct atggctgctc acaactgggc gacgatcaca tcaacacccg caccatgctg
     7021 caaaacatgg tgcgccaccc gaacgcgggc gcagtgctgg tgattggtct gggctgtgaa
     7081 aacaaccagg ttgccgcatt ccgtgaaacg ttgggcgata tcgatcctga acgcgttcat
     7141 ttcatgatct gccaacagca ggatgatgag atcgaagccg ggatcgagca tttgcatcag
     7201 ctgtataacg tgatgcgcaa cgataaacgc gagccaggca aactcagcga actgaagttt
     7261 ggtctggagt gcggtggttc tgacggtctt tctggtatta ctgctaaccc gatgctgggg
     7321 cgtttctctg actacgtgat tgctaacggc ggtactaccg tactgaccga agtgccggag
     7381 atgtttggcg cagagcagtt gctgatggac cattgccgcg acgaagcaac gtttgaaaaa
     7441 ctggtcacca tggtcaacga cttcaaacag tactttattg cccatgatca gccgatctac
     7501 gaaaacccat caccggggaa caaagcgggc ggtatcacca cgctggaaga caaatcactt
     7561 ggctgtaccc agaaagcggg ttccagtgtc gtggttgacg tgctgcgtta cggcgagcgt
     7621 ctgaaaacgc cagggctgaa cttgttaagt gcgccgggta acgatgccgt agcgaccagc
     7681 gccctggcgg gtgcgggctg ccatatggtg ctgttcagta ctggtcgcgg cacgccatac
     7741 ggtggatttg tgccgacggt gaaaatcgcc accaacagtg aactggcggc gaagaaaaaa
     7801 cactggatcg actttgacgc gggtcagctg atccacggta aagcgatgcc gcagttgctg
     7861 gaagaattta tcgacaccat cgttgagttt gccaacggta agcaaacctg caacgagcgt
     7921 aacgacttcc gcgaactggc gatcttcaaa agcggcgtaa cgctataaag tcgaaatgag
     7981 ttgttaataa aaaacggcgt ttcataatgt gaagcgccgt ttgcttttcc atacctgcta
     8041 aggatttatc atgaccgcgt attggctggc ccagggcgtg ggtgtcatcg cctttctgat
     8101 tggtatcaca acatttttca atcgtgacga acgtcgcttc aaaaagcagc tttcggtcta
     8161 tagcgccgtt attggcgtac atttttttct tctgggcacc tatcccgctg gtgccagtgc
     8221 catccttaat gccattcgta cattgattac cttacgcacg cgcagcttat gggtaatggc
     8281 gatttttatt gtgctgactg gcggaattgg cctcgcgaag ttccatcatc ctgtcgaact
     8341 attgccggtt atcggtacga ttgtcagtac ctgggcgctg ttccgctgta aagggctcac
     8401 catgcgctgc gtgatgtggt tttcaacgtg ttgctgggtg attcacaact tctgggcggg
     8461 gtcgataggc ggcacgatga ttgagggtag ttttctgctt atgaatggcc tgaatatcat
     8521 tcgtttctgg cggatgcaga aaaggggaat tgatccgttt aaagtagaga aaacccctcc
     8581 cgccatagac gaaaggggtt aaacaattaa ttacgcaggg cgctatttgc cagacgatcg
     8641 tcttccgcct ggcaagctgc cgcagtgaac agcacgtcag ttgaagagtt cagcgcggtt
     8701 tcgcaagagt cctgcaatac gccgataata aagccgacgg caaccacctg catggcgata
     8761 tcgttcgaaa taccgaacat attacaggcc agtgggatca gcagcaaaga accccccgcc
     8821 acgccggatg cgccacaggc acacagagaa gccactacgc tcaacagcag cgctgtgggc
     8881 agatcgaccg gaatacccag cgtattaacc gcagccagcg tcaacacggt aatggtgatt
     8941 gctgcgcccg ccatattgat ggtggctccc agcggaatag aaactgaata ggtatcgcga
     9001 tccagattca gcttttcaca cagcgccata ttcaccggaa tgttcgctgc agagctgcgg
     9061 gtgaagaagg catacacacc gctttcgcgc aggcacagca gcaccagcgg gaacgggtta
     9121 cgacgaattt tccaccacac cagcaatggg ttaaccacca gcgccaccag taacatacag
     9181 ccaaccagca cgaccagcag ttgcgcgtag ccccacagtg tggagaaacc ggtggttgcc
     9241 agggtagaag aaaccagccc aaaaataccg aacggtgcga agcgaatgac cagtttcacc
     9301 ataaaggtaa cggcattcga catatcattg accaggtttt tggtggtctc gttaccgtga
     9361 cgcagtgcga agccgaggcc gatcgcccac accagaatcc cgatgtagtt acctttcagc
     9421 agggcgtcga tggggttgga aaccatgctc attaccagtc cgcgcatcac ttcgacaatg
     9481 cctgacggcg gcgaaatatc acccgcgcta ctggacaagt gcagggtaga agggaaggca
     9541 aagctgaaga ctactgcggc cagagctgca gagaaggtgc ccagcagata gaggaacaaa
     9601 ataggacgga tattggtttt ctgcccgtgc tggtggttag caatagatgc cattaccagc
     9661 atcaacacca ggatgggggc aacagctttc agtgcgccga cgaacaaagt acctaacaga
     9721 ccaacagctt ccgccgcggg ttttgagatc catgccagaa gaatccccag aacaaggccg
     9781 accaggattt gttttaccag gctgccatga gccagacgcc ggaatagccc cggtgaacgt
     9841 tgcgtagtca tttttcgatc ctttcattgt gttgtcgcac atccctggtg cgttctgtcg
     9901 acgcatcttt caggatgtaa caaagtgttt gcaaggtcga gtataaggaa agttacagct
     9961 tcgggaagcg aaaaatgctg gattttaaag agaggtcatg ttttgcaact taatggttgc
    10021 atttatttaa cataaacaga gggaaggact ggctactaag gctgattaga taacatatgg
    10081 gcaaaactat ttggaatatg agaacggttg cgctccttta acgtgttatg ccatataaag
    10141 tcactggcgc agggagcgcg cagggggcgg ccaatcgccg ccgccccctg cacccccagg
    10201 ctctggcgaa caaaatcgcc gctgcgcggt tccctcagcc tatcccttgc ggctaccggg
    10261 tcgggcgcaa ggtaacgtcc ctgtaagatg cgccctcgcc ccggcctcca gggaacgctt
    10321 cggcgatttt gacgccacca acacccgtgc ggtctttttg taaagagcta taagccgatt
    10381 acataattaa tagctcacct ttgtctggaa tgagttatct ctaggaatgg cttaatatac
    10441 agcgaatcat tctccccgct gcttatcatg ccgataattc acccaggcgt tgataataaa
    10501 cgtcatcacc agaatgccaa acaccacgcc cagcgagacg gcgattggaa tatggtagaa
    10561 gtcgacaatc agcatcttga taccgataaa caccagaatc accgccaggc catatttgag
    10621 catcgagaaa cgctctgcta cgcccgccag caggaaatac atcgcacgca ggccgaggat
    10681 tgcaaacagg tttgaggtca gcacaatgaa cgggtcagtg gtcacggcga agatagccgg
    10741 aatgctatcc acggcaaaaa tcacgtcgct caattccacc agaatcagta ccagcatcag
    10801 cggtgtggcg tacagcaatc cattcttacg cacaaagaaa tgctcgttgt cgatggtgtc
    10861 ggtcatgcgc aaatgaccgc gtagccagcg caccagcggc ttgtcgccaa tgcctgattc
    10921 atcttcatgg gcgagcgcca tcttcacgcc ggtaaacagc aggaaggcac cgaagatata
    10981 caggatccag tcgaactgcg aaatcagcca gctgcctgtg aagatcatga tggtacgcag
    11041 aacaatcgcc ccgagcacgc catacaccag cacgcgacgt tgtaatgccg ccggaacgga
    11101 gaaatagctg aacaacatca gccagacaaa gacgttatcg accgccagcg atttctcaat
    11161 cagataaccc gtgagaaagg ccagggcctg tggatcggca acggcgcgac cttcggtttg
    11221 caccagatac caccagaaag cggcgttaaa cagtaacgac agcgtcaccc agaccagcga
    11281 ccaggccgca gcctgtttca tggtcatggc atgtgcccca cgacgcccct gcaacaacag
    11341 gtcgatagcc agcataatgg cgacaacaac agcgaatccg ccccatagca acggcgtgcc
    11401 gacagtattc atagaagttc cttacacata aaaaaacggc caacgtcgga agacgtcagc
    11461 cgctgctttt tatgcataga cctcgccttc cggcaaggtc tcacttacaa caaaaaagga
    11521 atacgtctgc gtattccttt cgggttgccc ggtgaccgga tgtggttttt cactcatcgt
    11581 aatgacgatc gaccggcaat gaagttactc ccctttgcag gtaacaaagt atgacagacc
    11641 attcagtcag tcaatggtct gttacatacc ttttacactg ttttactttg gcaagtatag
    11701 ttttacgcta tctgccggaa aaatcacccc agtttggcgg cggatctcgg tcagcagttt
    11761 ggctgtgatg cgactgaccg ccagccctgg atgatccacc agatgctcat ccaccagggt
    11821 agcgaacagc tctgcttcgt agagcatggt attaatatgc tgcggctggg tgagatcctg
    11881 catctggctg ccacgcggca caaagcagac tttctggcat tcagacagtt tttcaatcac
    11941 cagcgatccc gcttcgccct gaatctcgct tcccaggaca gaatcactga cctttgagtg
    12001 ctgcaaggtg acgctgaaat caccgtaatc catcaccacc acgccgtggg cgtcaacgcc
    12061 gcttgccagc aaactcgccg ttgcctgcac gcttttcggc tcaccaaata acgccaccgc
    12121 cgacgccagg cagtaaaagc cgatatccat aattgaaccg ttagagaatg ccggattaaa
    12181 ggtgttggga ttctcaccgt ccaggtaacg ttgataccgc gaggaatact ggcaatagtt
    12241 gaaaaagact ttacgcagtt tgcctgcttt cggcagcgcc tggcgcaaca aatgaaagtt
    12301 cggcaggcag gcggttttaa atgcctcaaa cagcaccacc tgattttcgc gcgcacaggc
    12361 aatggcggca tccacttccg ccagattcga cgccagcggt ttttcgcaaa tcacatgaat
    12421 tttatggcta aggaaaagtt gtgtctggga aaagtgcagg gaattcgggc tggcaatata
    12481 caccgcgtca atggcatcgc tttccgccat cgcttccagc gacgtaaaca gatgctcgac
    12541 agaaaaatca ttggcgaagt gctgagcctg ctcaaggctg cgggaatata cggcggttaa
    12601 cttgtattta ccgctctcat gggcggcctc gacgaactgg cgagtgatcc agttcgtacc
    12661 aatcacagcg aaacgtatca taacgcgtgg atactccata aagggattct gtcgtcaatg
    12721 tagcacgcct ttttcatctt cgacgtgcgc agatacgcat tattctttcg ccggacagcc
    12781 atgctcacta aacggcccaa tcatgaccga tggcccgaga tatttcggct ggcgatggag
    12841 gatttttgcg tcaatcaccg cgctgacacg agcgagtttc tcccgcagtt gagtataaaa
    12901 accgtgcttc atattgaggt ctggcgcgtt aaacccgata gcgtcgatgc cgtactgttt
    12961 tgccagccag atggtgcgtt ggttatgaaa ttcttgcgag ataatggtga tatggttttc
    13021 cccaaacacc tttttggcac gcactaccga atccagcgtt gagaatccgg catagtcgca
    13081 aaagatcact ttcgccggta cgcctttagc gatcaaagcc tgctgcatac ctgatgcttc
    13141 atcgtaattt ttacgtccgt tatcgccact caccagtagc catttcactt ttccggcgtg
    13201 gtacaacgct gctgcggtat caatacgtcg ggtaaagtag cgattacccg gcctcgcccc
    13261 cagcaataaa cccacgttac gcgccgggac ggcgttgaca tcgccccagg tcagctgctt
    13321 gctggcgttg accataacgc gatcggcgat aaaaatggtc gctcctgcca caagtaacag
    13381 taagcaggca atctttagag tccggcgaga aaaacaaata cggagaagaa ggcgggcaaa
    13441 tgcgcgcagc atggtcgtta tccttaacga aaggggggag gtcgatttta ggcgctaaag
    13501 aaagaaaaga taacgcctga catcattttc agattttgtc ctgcccaagc gacaactgcg
    13561 ttagccacag ccgggtgtcg aactcaagct ggtggtactg cggctccata tggcagcaca
    13621 attgatagaa cgctttgtta tgctctttct ccttcaggtg cgccagctcg tgcaccacga
    13681 tcatgcgcaa aaacggttcc ggcgcgttgc gaaacacggt agcgacgcgg atctctgctt
    13741 ttgctttcag cttgctgccc tgcacacgag aaacagcggt atgtaacccg agcgcgtttt
    13801 ttagcacatg gatcttattg tcatacatca ctttattgat cggcggggca ttacgcagaa
    13861 actgattttt cagatcctga gtatattgcc agagagcttt atcggtggcg tagtcgtgtg
    13921 ttcccggata gcgttttgcc agcacatcgc ccagacgctg ttcgttaatc agtgttcgca
    13981 cctgcgaaag cagctgctcg ggataaccct gaagataagt aagattgctc ataaccgccc
    14041 acgaaaaaga gaaaaagggt atactcacgc acccttttca ggggcaacgt cgaaatttta
    14101 tcattcagga gggccgatga gccacttaga caacggtttc cgttcactga cactacaacg
    14161 ttttccggcg acggatgacg ttaacccgct acaggcgtgg gaagcggcag atgaatattt
    14221 gctgcaacag ttggacgaca cagaaatcct cggcccggtg ttgatcctga atgatgcctt
    14281 tggtgcgtta agctgcgcgc tggcggaaca taagccgtac agcattggcg actcatacat
    14341 cagtgaactg gcgacgcgcg agaatttacg cctaaacggg attgatgagt cgagcgtgaa
    14401 gtttctcgac agcaccgccg actacccgca acagccgggt gtggtgctga tcaaagtgcc
    14461 gaaaacactg gcattgctgg aacagcaact gcgtgcgctg cgcaaagtgg tcacgtcgga
    14521 tacacgtatt attgccggtg ccaaagcccg tgacattcac acttccacgc tggaactgtt
    14581 cgaaaaagtg ctcggcccga ccaccaccac gctggcatgg aaaaaagcgc gcctgattaa
    14641 ttgcacgttc aatgaaccgc cgctggccga tgcgccgcag accgttagct ggaagctgga
    14701 aggtactgac tggactatcc ataaccatgc gaatgtcttc tcccgcaccg ggcttgatat
    14761 cggcgcgcgc ttctttatgc aacatctgcc agagaatctc gaaggggaga ttgtcgatct
    14821 cggttgcggt aatggcgtta ttggtctgac gctgcttgat aaaaacccgc aggcgaaagt
    14881 ggtgtttgtc gatgaatcgc cgatggcggt tgcttccagc cgtttgaacg ttgaaaccaa
    14941 catgccagag gcgttggatc gcagcgagtt tatgatcaac aacgcgctct ccggcgtgga
    15001 gcctttccgc tttaatgctg tgctctgcaa ccctccgttt caccaacaac atgcgctgac
    15061 cgataacgtc gcctgggaga tgttccacta cgcccgccgc tgcctgaaaa tcaacggcga
    15121 gctgtatatc gttgccaacc gccatctgga ttacttccat aaactgaaga aaattttcgg
    15181 caactgcacc actattgcga cgaataataa atttgtggtg ctgaaagcgg tgaagctggg
    15241 acgtcgtcgg taagattggg agttacctgc cggatgcggc gtaaacgcct tatccggcct
    15301 acgttcagca cgttaccccc aggccggata agacgcggca agcgtcgcat ccggcaatgt
    15361 ctgctcagtt ctggaatcgc gcttccataa aacaaaaaat ccctgtcacc atcctctaca
    15421 ttctcttgtt tagcgttttt ctacgtttat tcttccgtca cacagataaa ttcatccgtt
    15481 gccaatctgt caacggatgt tatcatattg ccaatttgtc aacggacgtg atgaagaatg
    15541 cacctgataa ctcaaaaagc attgaaagat gctgcggaaa aatacccgca acataaaacg
    15601 gagttggtgg ctctggggaa cacgattgct aagggatatt tcaaaaaacc tgagtcatta
    15661 aaagcagtat tcccatctct ggataacttc aaatatctgg ataagcatta tgttttcaat
    15721 gttgggggca atgaattacg tgttgtagca atggtctttt ttgaatcgca aaagtgctac
    15781 atacgtgaag ttatgacgca taaagaatac gatttcttta ccgctgttca tcgtactaag
    15841 gggaaaaaat gattgcgatt gccgacatct tgcaagcagg agaaaagcta actgctgtgg
    15901 caccttttct ggcgggtatt cagaacgagg aacaatacac ccaggcgctg gaactggtag
    15961 atcatctgct gctcaacgat cctgaaaacc ccttgctgga tctggtgtgt gccaaaataa
    16021 ccgcgtggga agaatcagcg cccgaatttg cggaatttaa tgccatggct caagccatgc
    16081 ctggcggtat agccgtgatt cgtaccctta tggatcaata tggtttaacc ctttccgatc
    16141 tgccggaaat tggcagtaaa tctatggtgt cacgcgtttt gagcgggaag aggaaattaa
    16201 cgctggaaca cgctaaaaaa ttggcaacgc gattcggcat ttctcccgcc ttgtttattg
    16261 attaacgcga cgtgccgaat gcgttgcatc cggcgcttca aataacgatt taaatctcca
    16321 gcgccagccg tgttccctgg gcaattgccc gtcgtgcgtc cagctccata gccacatcgc
    16381 agccgccaat taaatgcaca gttttcccgc tatcaatcag cggttgcgcc agcgcgcggt
    16441 ttggctcttg ccctgcgcag atcaccacat tgtccactgc taatacctgc gtttcgccgt
    16501 tgatcaccac atgcagcccg tcatcgtcaa tcttctgata acttacgcct gggatcattt
    16561 tcacaccccg cgagagcagg gtggtgcgat ggatccagcc ggtggttttg cctaacccct
    16621 gtcccggttt gctggctttg cgctggagca tcacaatttg ccgtgggcta cgcgggatct
    16681 gcattccctg cgggcttaag ccaccagcct gttgtaggct gctgtcgatc ccccactcat
    16741 tacagaacct ggcgatattc tggctggtgg attcgcccgg ctgacttaaa tacatcgccg
    16801 tatcaaaacc aatcccgcca caaccgatga tggcaacctt gttgcctacc ggcgctttgt
    16861 cgcgcagtac atcgagataa ctcaatacct tcggatgatc gatcccgtcg ataggcggaa
    16921 cacgcggcac gatcccactg gcgaggatcg tttcatcgaa cgcctgtaac tgatccgccg
    16981 tcacggtgtg attgagtttc agcgtcacgc ccgtcacttc gatcatccgg cggtagtagc
    17041 gcagcgtttc gtaaaactcc tctttgccgg ggatctgttt ggcgatatta aactgcccgc
    17101 cgatctcgct gtgcgcatca aacaatgtta cctgatgccc acgcgccgcc gcgttaatgg
    17161 caaacgccag cccagcaggt ccagcgccga ccaccgccag attttttttc tgcacggcgg
    17221 gaaggattgg cattttggtt tcgtggcagg cgcgaggatt caccaggcac gaggtgactt
    17281 tgccaacgaa gatctgatcg aggcagccct ggttgcagcc aatacaagtg ttgatctcat
    17341 cggctcgccc tgattgcgct tttgacagca gttccgcatc cgcgagaaac ggtcgcgcca
    17401 tcgatactat atcggcatcg ccgcgcgaga gaatatcgtc ggcaacctgc ggatcgttaa
    17461 tccggttggt ggttaccagc ggcagcgaga cgtggccttt cagtttgcgc gtgacccagc
    17521 taaatgcgcc gcgcggcacg ggcgtggcga tggtcggaat ccgcgcttcg tgccagccta
    17581 ttccggtgtt gataatggtc gcgcccgccg cttcaatggc ctgcgccagt tctaccgttt
    17641 cggcaaaagt gccgccgcct tcgactaggt cgagcatcga caaccggtaa ataataatga
    17701 agtcgttgcc gacgcgttcg cgcaccgtac gcactacttc tacggcaaat cgcatccggt
    17761 tgcggtaatc gccgccccac tggtcactac gctgattggt gcgcagcgtc agaaattcgt
    17821 tgatcaaata cccttcagag cccatcactt cgacgccatc gtatccggcc tcccgcgcca
    17881 gttgcgcgca gcgggcgaaa tcgtcgatca gttgcaggat ctcttcatgg gtgagttcgt
    17941 gaggaacgaa acggttgatg ggggcctgca atgcggacgg tgcgaccaga tgcggttggt
    18001 agctgtagcg cccggtatgc aaaatttgca gggcgatttt gccgccttcc tgatgtacgg
    18061 cttcggtaat ggtgcgatgg tgtgggatct ggctggcgtc gttgagcatt gcgccacctt
    18121 ccatgccaac gcctgttaaa tctggtgcaa tgccgccgct gacaatcagc gccacgccgt
    18181 gacgggcgcg ttcggcataa aacgcagcca gccgttcagc accgtccggg tattcctcca
    18241 gcccggtgtg cattgaaccc atcaacacgc ggttttttaa cgtggtaaaa cctaaatcca
    18301 gcggggcgaa cagcgacggg tagctcataa aattgtccag tatgtaaaat aattgttatg
    18361 tagtcggatg agttctaatt tagccgtcga tggagaaaag ggaaaagggg agtgcggggt
    18421 ttgtgatggg attcaaaaag aatacgcccg gtggtaacgc cggatgcgac gcttgccgtg
    18481 ttttatccgg cttgggggat tgcacatgta gggcggataa ggcgttcacg ccgcatccgc
    18541 cagccgttgc aaattactgc ttacggaaaa aatcgttata caacatatac aaatgagcgg
    18601 cactccagga gaaatttggt gcgccttgct gcgcgcctgt cagcgggttg taattctcct
    18661 gaattgggcc atcggcggtt aatcctttgg cgtgcctgaa gaacgtatcc gccagcttca
    18721 gggcatcatc acgataaccg taacgctcca tccctttcag accaaaccag aactgatcca
    18781 cccatacgcg cccgcgccag tagatatcag cgccaaaagc cggattggtt aacgccgccg
    18841 ttcccagcgg aacaaatgtg ttgaactctt taggatcgag catcaccttc accaccgcgt
    18901 cggcattggc ctgcgttgcc gcaccgttaa acagcggcga ccagccttcc ggccctttac
    18961 cgcgctcaac aatcggtttg cccgcgcagc cgttcgccag tggtttatct tcaatacgta
    19021 cgtcatagta gaactgggta gtcgggtcga acatacaggt gttgatgtag tccgcgagct
    19081 gctgtgccaa ctggcgatag cgtttggcct cttctggctt accgaggatg gttgccatct
    19141 ccgccagata atggttatcg ctgtacatat agctggcctg atccaccgac tcctgcaata
    19201 gcgagtagcc cagcaacgtt ccgtcctgac tgcggttttc ggcgaatttc accgtccagt
    19261 cgctacgttt gccgccatta gcgacatatt tatccaactg ttctttgtcg ataaaaccaa
    19321 agacggcggc atcatcacgc cccgattccc acgacgcagc gacctgtgcc ggaatttcca
    19381 gactgtcgta ctggcctttc tccaccacgc gggcgtagtt gttcagccca gactgcgtct
    19441 cttctttatc gccttttttc accgtaaaca gcatctcgcc gctctcggtg ttgtgggctt
    19501 tgtcgcgggt cgcgccatat tccggcaccc cgttgccgtt atgatcgcgg ttacgtaacc
    19561 accagtcgtg ataggccacc agtttcgggt acatctctgc caaccaggct ttatcctggg
    19621 tgacgttgta tacttccatc accgaccagg cggcaaggct gggtttggta ttacgttcgt
    19681 tccagttgcc gccatcgcca ccacgctcgg ggctaaggtt ccacgctatc aggtcgggga
    19741 caaagcccac atcctgcgga cgcacgctgt cgccaggctg gatctgccag gagaagaccg
    19801 cgcggatatt ctctttggcg atatccggat tgaaatgcgc catcgcaaac gcctgcttcc
    19861 aggtatccca cggccaggtc tgattgccgg agaaccagcg tccggtcacc gacggcgtga
    19921 cggtgttata attcaccgca ccgcccggtg agcgccagtt accgttgagc gtttcgatcg
    19981 ctttcaccgc gacgcgcgtc tgttcctgcg tcgcatccgg attggttaac cctttcttca
    20041 ggtattcttc ccagcgttgc tgcgaggcgg tgagataaaa cgccggacgc gccagaatat
    20101 cgcggatctg catttgctct ttgctaactt cctgagcggt cagcagatgg gaataggtgg
    20161 tgtagagcgt ggtcgaaccg ttgatatgcg ccttactggt aaagcgatta ccgttgattt
    20221 cagtctgcac cggcagggat ttatgcacct gatattccga ttcgccggag gtcagcagat
    20281 cccaggtggc gcgcactttg ccaaaggtga ctttcaggcc atcacgggtg gcgctgattt
    20341 tgcgctgata gtcagggtat tcgccagcaa tggttttatc ggaaagcggt ttcccttctt
    20401 tcgcttccag tttttccagc agttcgccat cccacaccag atccagcggt ttattgctga
    20461 tgattttagt ttccagtagt gacgtgcgcg gcgtggcgaa gcgcagagtc atttcgacct
    20521 gcacatcttt tgctgtcagt ttttgcacca gcgcgccggg aatactgtat gcctccagcg
    20581 tgaagttgac tttcttgccg tcttgccaga cagtcaggcg gtcgaaattg ctggccataa
    20641 agttgatgta ttcttccgtc agcagcgcaa cgcccggaaa gccgcccatg gtgttcgggc
    20701 cgtctggcaa cagatgacca tgccaggctc cgagatcgaa aaacggatta aaacgctggt
    20761 gatcgtcgta atcgtaatcc ttcatgtact gcggcgcacc agtacggtta atcacgtttt
    20821 tataattgtc ggcgttagcg gcatgagcgg aaaaacttat cagcagagcg caggttactg
    20881 gcgttaaaat agttttgatt ttcatgagat tacagtcctt attaccagta gaaatattgc
    20941 atcaggaaga tttggtcgct gggatcgtta gaggcatttt tcatgtagaa gcggctatca
    21001 aaagccgtgg cgaaacgtcc gccgccatat tcgtaccaga taccaaactg catgaaagag
    21061 gtggtttcat cgtcagcgtg gtcagggcga tgtttggcgt aggtgtaagc ggtggagaga
    21121 taaacgcctt ttaacgcttc atacatggcg ctcaccatca tcccgctttc acttcccggt
    21181 acgtccagcc cgtcggcacg tccggtatgg tgccaggcgc gagcggcaaa gttaggggta
    21241 aacaggcccg tgagatagag ttgtcctgtg ccgtcggtaa tttccagacc attcatccag
    21301 gtgatgcctt cggcaaggtc atactggatg taaccgttca tccattgccg gataggtgta
    21361 tttgtcgtcg tagcgatcgt acttgccaaa atgtagccag gctttgcttt cgtcatgatg
    21421 tccggcaggt gtggcggtga cgctataacg aaagttgccg cttaagttct gtaccttcag
    21481 cgcggtcata atgtcgcggg tattgggaat gacgtagccc aaatcgggtg tgaagtcgcc
    21541 ccaccattgc aggtcatcca gcgaggaatc ctgacgcaga ctgagcatga tttcggtgcc
    21601 atcattggta cgataaccgc catagaaacg gttaatccca ccttcgaaac caccccagct
    21661 atggtcaggc acctaggcat taccgtcgtg gtcggcctgc acggtccagc cttcgccgta
    21721 aatcaggccg aaccaggagc cgtacttcat ctccaggcca ccttcaatat aggtgccgtc
    21781 gctgtattta tgtttattct caccctctaa atccatatat ccaccaagcc ctaattcgcc
    21841 gtaaaaacga aatgaaggag aagatgtcga ggtaattgcg ggtgtttttg tgtcgtcatg
    21901 ttctgccgga cgtgcttctt ctcctgccgc agaaaaggcg tagcaaaacg gcagagcaag
    21961 taatgctctg catggtgcag tgattaattt cattgtgtta atccatgagc taaagaaaaa
    22021 gtacagcctt ttatcgttgt aattataaaa atataaaggc agaaactatt ttccctctcc
    22081 tcatccagga agagggaaaa tgcctgctca ttttttatta gtctgcttaa tcaacattgc
    22141 tggctggcgt cgcttcaata tggcgctctt cagccgttaa tccctttata tatttgctgt
    22201 atttccacca cgcaaagccg aggaagataa caataccgcc gacgttataa aaaatgatgg
    22261 tcagaatatt cgcgccagtc gggaatgtcg aagcgacaaa ccctacggca aatatcgcaa
    22321 tcagcattga aacaacgata ataccggtgc ggcgggagcc catacggaaa tcgcgtggca
    22381 ggtgatctaa tttggcgcgt aaattcaggt aagccagcat gatgaataac ggcggaagca
    22441 tggacgctgc ggcggtcata ttaataatgg tattcatcag atcctgcacg gtattggaac
    22501 cgagcatcgg gataatcatc agcgggatga cgatcagaaa ctggatccat gctgcgcgcg
    22561 ccggaacgcc gttttcgtta agttcgacgg ttttcttacc aaagatgcct tccgggattt
    22621 cggagaagaa aattttcacc ggcgttgcgg tccacatcag cagggaaccg aacattgcgg
    22681 taaaggacac cagaccgaca aagcgattca tcaacgcttc cggcagacca aaatacgccg
    22741 ccatgccgtg gaatacctgc accgatccgc cggtaaattt caactctttg ctgctgacga
    22801 agacgttaat cagcaccgag gagacggaat acagtacgcc gataaaaatc ccggcgagga
    22861 tgatcacttt aacgaacgat ttcgaaccgc ctttgacgtc gttaacgtac accgcgacgg
    22921 actccgcgcc acctgcggcc ataaagatcc aggtggtaac gccgaggaac gcccagttga
    22981 agttcgggat catcgcgtca acggtgatcg gatcggcagg ctgtacgccg ccaaccagtg
    23041 ccgtacctgc cagtaaaatg taggagagcg ttaacagcag catcagcgtt gaagtgacgg
    23101 aggtaattgg ccccagcatt ttcgccccgt tggtggaaac ccaggtggag aaggcgaaca
    23161 gcaccatact gataacggtg gtggcaaccg gcgtcataat atattcgtag ccgaggaagg
    23221 catacgaagc ataagcaata acgcgcggca acagcgaggt gaaaaagaac agatttacga
    23281 accagtaagt gtaggcagta ataaatgccc aacgtccgcc cagcgaactt tttacccacg
    23341 cgtagacacc ggcttctgag tttttattta acgaaacaaa ttctgcgatg atcagacaga
    23401 agggaataaa ataaaaaatc gtcgcgagga aaaacatcgg tgccgaggcc agtccaagct
    23461 caatattatt attgataacg ttgttaaagc tgtaaacggc ggcaaaagtc agcgagagca
    23521 agccgaattt gccgattgta ttacgtttgg tatcagacat aacacatctc cgaaaatgtg
    23581 tagggtagcg gtggcggcaa actccttttg ccgccgtagt tgttatttgt tatggaaata
    23641 accatcttcg atggtgactt tgagaaccac ttttttgacc gcgttattgc agataaaccg
    23701 atacgcttca tggatatcgc aaataacgat ttgcccttcg tggacctcta cggtttctcc
    23761 gcagcctttt aaatattcac ggtcagtttc atcacgataa tattccacta cctgtaatgt
    23821 ctctttcggc gcatattcaa ttttttgctg cccttgcagg tagtaatgca cttcaaaata
    23881 gcgtcgatgc ccggtaaata gcgcatcggt cgcggagtca ttttccacac ggtaggtcaa
    23941 tgaatcaccg atggagtggg cgacgccagg ctgaatgttg tcgatatttt caatcgcttc
    24001 aacgcagcgt tgccacttct tgccagaggc gtaaatctgg cggaactgtt ctaagttatc
    24061 gatgatcctc attgcttatt ctcgctgtgc aaattcgtgg aaaagaaccc tgcgccgaac
    24121 tcatacgacg ccaggctttg cgcggtagct tctccgccag aaaccggcag caacgtaaag
    24181 ccgtagctga agtcacggaa ccagacgcgc caggagtcca gcacctcgct gccccaggag
    24241 ttggagccga ggccaagtag ctggtggtcg aggttcaggg tgatgtcatc actgcgctgc
    24301 agctcgttac agtgctgggc agcgtggatg ttttcctggg tatagtgcca ggcgctgaag
    24361 ttaattgggc gctgcggaac caccagcaga ccgttgccgt ggcggttagt cagtgccgtc
    24421 cagcggacat gctgacggtt gccgttgttc tgcgggaagg gatagttctc gaacatggca
    24481 tcgacggtgc tgcgccagat atcgatgatg ttagcctgct ggctgtcggc gtagttttcg
    24541 cccggtccac gaccgtaata cgccacctga tcgtattcgc cgttaatccc catggtgaaa
    24601 ccgatgcacg gaatgatgtg cggatagtcg ccgtaacgct cgccggaaag cgccacgtta
    24661 acctggccat cggcagcgat gcgccagatg taggtacagc gcatcccgaa gtcaaacacc
    24721 ggcggtgcaa taactgtgcg gctgatgatc aacacttcac catcgctctg ttctacggca
    24781 aagtcgcgca gatgttcctg catgatctgc aaatgattcg gctgccacaa cccttcgtac
    24841 tcctgcttgt ggttgtcgat catcggcttg aagaagttga tctttggctc gcgggtcagc
    24901 agcgactcgc catttacctg ccaggatgtc ggtttgccac tcatttttga gaaggtgatc
    24961 gcgaagttgt agccgcgaac ggtgcagctc aaacgatcgt cttccagcgt cagcggacgc
    25021 gcattatttg gtgcgaaagg cactggctgc gcggtgtttt ccttcagcgg gaactgataa
    25081 gtggcgatag aatgcccggc ttcgctgtag cgggtgcggg aatctttggt caccgtaatg
    25141 ttgaggaacg cttcgcgggc gtccagctgc ggcagcgtga tctgcaaggg ggcttcgctg
    25201 ttcggcgcaa cgtcgcgcag tttaatctgc tgcgtcgcga gcgtttcacc ttcggcgcgc
    25261 acctctgcgt gcagggtgta gtcatcaagc gtggtaaacc acagtttatt ttcgactttc
    25321 aactcgccgc gagtcagcgc gtggattttt accggcgcga taacctgttt gtactctttc
    25381 aggcccgatc ccggcgtctg atcggaatag atcaaaccat caagacagaa gttatagttg
    25441 ttgggatagt cgccgtagtc gccgccgaat ttataccaga cattgccgtg gtcgtcctgt
    25501 gcctggatcc cgtggtcgca ccactcccag acataatgac cctgaatgca atcgtgctta
    25561 tagaagacgt tctggtactc cgtcagcccg cccggtccgt tccccatcgc atgagcatat
    25621 tcacagatga tgcgcggctt cggatgcggg tattcaccaa actcattcat cagcggcacg
    25681 cgggtgtaca tggtggaaat aatatcgacc acttcagcat cgcgatcttc ttcgtaatga
    25741 accagtcgcg tgtcatccag cgctttcgcc gcatggtaca tcgcgcggat gttacagcca
    25801 tagccggatt cattgcccag cgaccagatg atgatcgacg gatggttttt ctgtgcgtgg
    25861 atatggcgaa caatgcgctc gacgtagact ttttcccact gcggatcgtc ggtaatacgg
    25921 ctgatatcgc cgacattagc aaagccgtgc gattcgacgt cggtttccgc catcacaaac
    25981 aggccgtaga tatcacacag ttcgtaaaaa cgcggatcgt tcgggtagtg agcggtacgc
    26041 acggagttga tattgtgctg cttcatcaac tggagatctt tctcgacgcg atccattcca
    26101 acggcgcggc ctttgcgatg atcgttgtcg tgacggttga cgccgtgcag catcacataa
    26161 cggttattga tccagaacag accgtcgcgc actttgatat cacggaagcc aacgcgttgt
    26221 ggcaccactt ccagaacgtt gccgttggcg tctttcagcg tcatgaccag atggtaaaga
    26281 taaggggatt ctgctgacca ttgctgcggc tgttcgacag taaaagcaaa gctggcgctg
    26341 gtcagttttt caattgccaa atgatcaatg gcgctgctgt gcaccacgcg ttcgccatca
    26401 aacagggtat attccagcgt cgtgacgaca ggggaggcgg cgagattttc cagcaccact
    26461 tcgcaggaaa gcgtggcatc gcaataggct tcgtcaaagt cggtacgcac agtgaaatcg
    26521 ttaatatgcg ttaggtgttt tccgaccaga taaacatcgc ggaagatccc cgccgaccac
    26581 cacatatcct ggtcttccac gtaggtagag tccgcccact gcatcacgcg cacacacaac
    26641 aggttgtcgc cggttttaac catcgcgctg atgtcaaact ctgcggtcag gcgactgccc
    26701 ttgctgaaac ccacatactg accgttaacg tagacttcaa aataggtttc gacgccgtca
    26761 aatttaatca gcgtctgttt accctgccag ccgtcgctga gggtgaaaat acgttgatag
    26821 gcaccggttg ggttatcgct ggggacaaac ggcacatcga tggggaacgg aaaaccttcg
    26881 tcggtatatt gcagtttgcc gtgaccttcc atttgccaca tggcggggac ggtaatatgc
    26941 ccccagtcag ccattaactc agaggtgaag gcttctggta cttgcagcgg atggtcaaaa
    27001 aagtggaaat tccactgact gcttaagggc agaaacaggc tgctggtttc gcgggcaaag
    27061 gtacgcgctt gcgcaacaga atcatatgaa aaaaagtacg cacgcggcgc aagtcggttt
    27121 tcgtgggtga gctgaatgtt ttcccagcga ttcatagggc ctcccggtag agagaaatcg
    27181 gaaccattca tggtgatggt agtttgtgta attgctgggg gtttagtaaa atttttacta
    27241 aaaagcagca ggtaaatgca tttactttaa ctggatcacg aaaataaacg gcagcactgt
    27301 ccggcaaaaa tgattgccga acagtgtgta aggggattta acgggtcgtg ccgcgcagtt
    27361 ttaatttgct gggaacgaag actagcagcg gcagcgcgcg accatcgcgg gctttttcat
    27421 acaccaggtt aacgccctga cttcccatca tctcggaatg gatgcgcacg gtggagagcg
    27481 gcggaaaggt aaatcgcgcg gtggggatat cgttaacgct gataagcgaa atatcttgtg
    27541 ggatgttcag gccacgttca tgaattgccc gcagtacgcc gatagcaatg gaatcggaag
    27601 caacaaacag tgccttcgga tagtcttccc gcgccagcat ttgttttgcc agttcataac
    27661 ccgacgaact ggaaaaaccg ccgcgccaga tatcctcttc gcgtaccact tgtttcagtc
    27721 ggccatattc cgcaaaggcg acctcacgaa tatccgcctt gccaggctca tcttcaccgc
    27781 caataaaacc aatacgatta acgccctggt tgatatagaa gtcgatgatt tctttactga
    27841 tgcgtgccag atcgatatcc accgcatcgt aaccgctgcc gggttcgtga aagtcgataa
    27901 aacagatatt gtcggtcaac gcgctggcag cggcgcgcag ggcgggcgtg ggtttgccga
    27961 caattaaaat accggtgacg tttttaatgt ctggtaatcc gttgtgttca taacagttgg
    28021 tcagttcgat cgccagcttt tcgcactgag tttcaatgcc gtggcggatc gccagatagt
    28081 aaggatcgtt gatctccagc tcctgctggt agctgtagat agccagaata tggtgttggt
    28141 tgactgcgcc tgtctgtagt ttacgggcgc tactggtctt gtactccagc ttttcggcga
    28201 tctcgagaat gcgatgtttc gtctcttctt tcacattcaa tgtcggatcg tcatttaaga
    28261 ccctggatac tgtcgccagg gatacgccag cttcgattgc gatgtctttt aatgttgcca
    28321 tgtttacctt ttttactttc cctgatgtgc gcccccattg tagaggaagt gcttatcggg
    28381 tcacctgttt tcagtaaata aagcgcgttt ctttgacgct gtaacggtaa cacagcggct
    28441 taggaatgtg atcacgcctg cattctccat ttttagccta caggattgag aaaaactttt
    28501 ctatagttta gtaaaatttt actcgaaggg aggaagcaat ggaaaccgtg gcttacgctg
    28561 attttgcacg tctggaaatg cgcgtcggaa agattgtgga agtgaaacgc catgaaaacg
    28621 ccgacaagct gtacatcgta caggttgatg tggggaaaaa acactgcaaa ccgtgaccag
    28681 cctggtgccg tactacagcg aagaggagct gatggggaaa acggtggtgg tcttatgcaa
    28741 tctgcaaaaa gcgaaaatgc gtggtgaaac gtcggaatgc atgctgttgt gtgcggaaac
    28801 cgatgacggc agtgaaagcg tgttgttaac gccggagcgg atgatgcctg cgggcgtacg
    28861 cgtcgtataa aaagatcgga tggcgacgtc gtatcgccat ccgatttgat attacgcttc
    28921 ttcgacactt actcgcatgg ccgccagcgc cttacgcgcc gctttgatca ccagttcaca
    28981 ctgttcaatg gtcagtgtca gtggcggttc aatgcggatc gttttggcgt tattgagcgt
    29041 tccggccacc agtacgcgct ggcggaacat ctcgctggca aagttatagc cgatttcgtt
    29101 atcaacaaac tcaatcgcca tcaacatccc tttaccacgc gcttcctgta ccagatcggg
    29161 atattcccgc gccagttgac ggaaaccgtc cagcaacata tcgccttttt gctcagcctg
    29221 agccggtaag ttctgctcca gcaacacatt gatggtcgcc agcgccgccg cacaggccag
    29281 cgggttgccg ccaaaggtgg tggtatgcag gaatgggttg tcgaacagaa ctgaaaacac
    29341 ctcttcagtg gcgatggtcg cgccaatcgg catcacgccg ccgccgaggg cttttgccag
    29401 acacagaatg tccggctgaa cgttctcatg ttcgcaggca aacatcttgc ccgtgcgccc
    29461 catgcccgtt tgtacttcat cgaggatcat cagtgcgccg aactcatcgc acagcttacg
    29521 cacggcggtg agatagcccg gcggcggcag aattacgcca ccttcgccct gaatcggttc
    29581 gaggatcacc gcagccacat catcaccggt ttttttgcac tcattaagag ccgtgcgcat
    29641 ggcttcgata ttgccaaacg gcacatgacg gaaccctggc agcagtggca taaacggttt
    29701 gcggaaggtc gatttcgccg tggccgacag cgcgccaagt gatttaccgt ggaacacgcc
    29761 gctggtggca ataaaagtaa atttgccgcg cggcgactga taagccttcg ccagcttcag
    29821 cgccgcttca acggactcgg tgccgctatt acagaagaag ctgtatttca gtttaccggg
    29881 cgttagcgca gcaagggttt tcgccagcat agcccgtaac ggatcgagta gctcctggct
    29941 gtgcagtggt tgtttcgcaa gttgattctg tacggcggaa accacaactg gattacggtg
    30001 ccccacgttg aaaattccaa aacctcccag gcagtcgata aactcctgtc cctgggtgtc
    30061 gacaagcgta tttaaacctc ccgcttgcca ctctacggct ccgtaatccc cgccggcggt
    30121 aacagatttg cgatactcta aaaaccccgg attgacatgc tctttgaagt attcaatcac
    30181 ctctcggtta agtgctttca tctcctcatg atccagcgtt cgcttctcaa tgagattcag
    30241 ggcgtgggcg ctgcacgcta aagccgatgc gctcgaaggt aacctgttca aaatatgctc
    30301 cggaggctcg cgtatcacat gataccgatt taagtattgc agggattgcg ccactccggc
    30361 tgcgatcgcg aaaatcggga taaacacaaa tgcggttatc atttgtgcaa tatttaatgc
    30421 tatccgttaa catttgttgt tattttgcac ggtttttgtc gggtatggat ttgatgattg
    30481 ctgcgcaaat gcacgttttc agggcaataa ttgcctgatt tttgggcaag ataattaacg
    30541 ttttcgtata gtaacagagt gaaagtattt taaatcaaag aattagattt gtgctttaat
    30601 cgcaaattgc gatctaaatc aaattaatcg gttaaagata accgcagcgg ggccgacata
    30661 aactctgaca agaagttaac aaccatataa cctgcacagg acgcgaacat gtcttctcat
    30721 ccgtatgtca cccagcaaaa taccccgctg gcggacgata ccactctgat gtccactacc
    30781 gatctgcaaa gctatatcac tcatgctaat gacacttttg tgcaggtgag cggctttacc
    30841 ttgcaagagt tacaagggca gccgcacaac atggtgcgtc acccggatat gccaaaagcg
    30901 gcgtttgcgg atatgtggtt caccctgaaa aaaggggagc cctggagcgg catcgtgaaa
    30961 aatcgccgca aaaatggcga ccattattgg gtgcgggcca atgcggtacc gatggtgcgc
    31021 gagggaaaaa tcagtggcta tatgtcgatt cgtacccggg cgacggatga agagattgcg
    31081 gcggtggagc cgctgtacaa agcgttgaac tccggacgta ccagtaagcg tattcataaa
    31141 ggcctggtgg tgcgtaaagg ctggctgggt aaactgcctt cattaccgct tcgctggcgg
    31201 acgcgtggag tgatgaccct gatgtttatc ttgctggcgg ccatgctttg gtttgttgct
    31261 gccccggtgg tgacgtattt cctctgtgtg ttagtggtat tgttggcaag cgcttgtttt
    31321 gaatggcaga ttgtgcgccc gatagaaaat gtcgcccgtc aggcactgaa ggtggcgacc
    31381 ggagagcgta atagtgttga gcacctgaat cgcagcgatg agctggggct gacattacgc
    31441 gcggtagggc agcttggcct gatgtgccgt tggctaatta acgatgtctc aagccaggtg
    31501 tccagtgtca gaaatggcag tgagacgctg gcgaaaggca ccgatgaact gaacgaacat
    31561 acccagcaga cagttgataa cgttcagcaa acggtggcga ccatgaacca aatggcggcg
    31621 tcggtgaaac agaactctgc cacggcgtcg gctgccgata aactttctat caccgccagt
    31681 aatgcggcag tgcagggtgg ggaggcgatg accacggtga tcaagacaat ggacgatatc
    31741 gccgacagta cctagcgcat tggcaccatt acttcgctga ttaacgatat tgcgtttcag
    31801 accaatattc tggccctgaa tgcggcggtg gaagcggcgc gtgccggcga acagggcaaa
    31861 ggttttgcag tggtggcagg ggaagtgcgt catttagcca gccgcagcgc taatgctgcc
    31921 aacgatattc gcaagctgat tgatgccagt gctgataagg tgcaatccgg ttcgcagcag
    31981 gtacacgccg ccggacggac gatggaagat attgtggcac aggtgaaaaa cgtcacccag
    32041 ttgatcgccc agattagcca ttcaacgctg gaacaggccg atgggctttc cagcctgacc
    32101 cgtgcagtgg atgagcttaa cctgatcacc cagaaaaatg ccgagctggt ggaagagagt
    32161 gcgcaggtgt cggcgatggt gaaacaccgc gccagccgac tggaagacgc ggtgacggtg
    32221 ctgcattaat cggtatgccg gatccggcgg ttggagcaca atgcctgatg cgatgctggc
    32281 gcatcttatc aggcctgcgg ggtgtgtagc gggtgtaggc cggataaggc gcgtcaagcg
    32341 tcgcatccgg caattgcacc gcgccactgg cggatgcggc gattaatcca gttgcgtaat
    32401 atcaaaagcg gcgcggtcga tcaccgcaat gatctttttt atttgtgcat cactgatatc
    32461 gctctggttg acgcgtaaat ccagcaccgc tttaaaatta tccagcgccc gcttcatttg
    32521 cgggttctgg cgcagcgccg cgccaacgca acgcgctttg atgcgttctt caatcatctc
    32581 cacttgttcg cggttttctt ccagccactg cgcgccttgt tcggtcagcg caatctgctt
    32641 cttaccacct tcctcttcgc ggatggtaat cagcgactgc tcctgcagaa aatccagcgt
    32701 cgggtagatg acgcccgggc ttggggtgta attcccctgg gttagattct caatcgcttt
    32761 aatcaattcg taaccgtggc tgtcatcgcg cgagagaata tccagaatca ccagacgcaa
    32821 ttcaccgtga ccaaagaaac gttgccgacg accgccgccg cgaccgtggc gaccaccgca
    32881 acattgaccg tgttcatgct ggtgaccgtg tccgcagtgc tcgtgttctg acttctcacc
    32941 tttgcagcag ccctcatggc gtggctggcc ttcatgttta caacaccctt cgtgatgatg
    33001 gctcatatca gcctcctttt gcttgattag atatatctaa tttatatctg atgtgagcat
    33061 tactgcaagt acaaaaatga tttttaaacg tggaagcacc tcacatctag gccggataag
    33121 acgttcacgc cgcatccggc agcattgcta tggcactgaa tgccttcaca tacttcagac
    33181 atatcagttc tacaaatcgc ttgcatttat catgattaac aatcattatc atttgcgaat
    33241 tttatttaga tatatctgat taccatcacg aaggcgataa caatgaataa caccccccgc
    33301 tacccgcagc gcgttcgcaa tgatctgcgc ttccgtgaac tgactgtgct acgcgttgag
    33361 cgcatcagcg ccggttttca gcgcattgtc cttggcggcg aggcgttgga cggttttaca
    33421 tcgcgcggct ttgacgatca cagcaaactc ttctttcctc aacctgacgc tcactttgtg
    33481 ccgccaacgg taacggaaga gagcatcgtc tggccggaag gaccacgccc accgtcgcgt
    33541 gactatacgc cactgtatga cgaactacgc catgaactgg cgatcgattt ctttattcac
    33601 gacggtgggg tcgccagcgg ctgggcgata aacttacggt ggcaggtccg cgcggttcgc
    33661 tggtggtgcc ggaagattac gcgtatcagc tgtatgtctg cgatgaatcc ggaatgcccg
    33721 cactgcgccg ccgcctggaa acgttgagca aacttgccgt taaaccgcaa gttagtgcgc
    33781 tggttagcgt gcgggataac gcctgtcagg attatctcgc gcaccttgat ggttttaata
    33841 tcgaatggct ggcacatgat gagcaggcgg tagatgcgcg tctggcgcag atgcaaatcc
    33901 ctgccgatga ttacttcatc tggataaccg gcgaaggtaa agtcgttaag aatttaagcc
    33961 gccgctttga agcggaacag tatgacccac agcgggtacg tgcagcggct tactggcacg
    34021 caaagtaact gatatataaa aagaaaaagg ctgacgattt ctcgtcagcc tttgctatat
    34081 ctggtggccc ctgctggact tgaaccagcg accaagcgat tatgagtcgc ctgctctaac
    34141 cactgagcta aggggccatg gtagcggatt ataaagtaac tccgtgccgc aatccagcca
    34201 ttaccgcgcg cctgctgttt ttataaacaa tgtattttca atccgttata cttttcctgg
    34261 tgatgttaga aaggagtaaa tatggttgag gatattttgg ctccagggtt acgggtcgtg
    34321 ttttgcggta tcaaccctgg gctttcatcc gccgggactg gttttccctt tgctcatccg
    34381 gcaaatcgct tctggaaggt gatatatcag gccgggttta ccgaccgtca gttgaagccg
    34441 caggaggcac agcatctgct ggattatcgt tgtggcatca ccaaactggt agaccgtcca
    34501 acggtgcaag ccaatgaagt ttcaaagcag gagctgcacg caggtgggcg taagctgatt
    34561 gaaaaaattg aagattatca gccgcaggcg ttggctattc tgggcaaaca agcatatgaa
    34621 cagggattca gctagcgcgg tgcacagtgg gggaaacaaa cgctcaccat tggttcgacg
    34681 cagatttggg tgctgccaaa tcccagcggt ttaagtcgcg tttcactgga gaaactggtt
    34741 gaagcgtatc gcgagctgga ccaggcgctg gtagtgcgtg ggcgataaaa aacgtcaccg
//
LOCUS       DAZPCS010000028        32041 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZPCS010000028 DAZPCS010000000
VERSION     DAZPCS010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32041
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.028"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..271)
                     /locus_tag="ACHUQ7_001337"
     CDS             complement(<1..271)
                     /locus_tag="ACHUQ7_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HFR1134820.1"
                     /translation="MVKKPQQGTIFAAAQRSDSYFVKCINMLSLYEKIRLIILFLLAA
                     LSFIGLFFMINYQLVSERAVKRADSRFELIQKNVGYFFKDIERSAL"
     gene            complement(371..1282)
                     /gene="gsiD"
                     /locus_tag="ACHUQ7_001338"
     CDS             complement(371..1282)
                     /gene="gsiD"
                     /locus_tag="ACHUQ7_001338"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFR1134821.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1285..2205)
                     /gene="gsiC"
                     /locus_tag="ACHUQ7_001339"
     CDS             complement(1285..2205)
                     /gene="gsiC"
                     /locus_tag="ACHUQ7_001339"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFR1134822.1"
                     /translation="MLYYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMFLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2223..3761)
                     /gene="gsiB"
                     /locus_tag="ACHUQ7_001340"
     CDS             complement(2223..3761)
                     /gene="gsiB"
                     /locus_tag="ACHUQ7_001340"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFR1134823.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPTALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(3781..5652)
                     /gene="gsiA"
                     /locus_tag="ACHUQ7_001341"
     CDS             complement(3781..5652)
                     /gene="gsiA"
                     /locus_tag="ACHUQ7_001341"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFR1134824.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(5639..6604)
                     /gene="iaaA"
                     /locus_tag="ACHUQ7_001342"
     CDS             complement(5639..6604)
                     /gene="iaaA"
                     /locus_tag="ACHUQ7_001342"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFR1134825.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            6808..8043
                     /gene="moeA"
                     /locus_tag="ACHUQ7_001343"
     CDS             6808..8043
                     /gene="moeA"
                     /locus_tag="ACHUQ7_001343"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFR1134826.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKMILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVREWVEVEPFNALFGGL"
     gene            8043..8792
                     /gene="moeB"
                     /locus_tag="ACHUQ7_001344"
     CDS             8043..8792
                     /gene="moeB"
                     /locus_tag="ACHUQ7_001344"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFR1134827.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(8971..9633)
                     /gene="fsa"
                     /locus_tag="ACHUQ7_001345"
     CDS             complement(8971..9633)
                     /gene="fsa"
                     /locus_tag="ACHUQ7_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFR1134828.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVTKFEQDWQGAFGRT
                     SI"
     gene            9764..10663
                     /locus_tag="ACHUQ7_001346"
     CDS             9764..10663
                     /locus_tag="ACHUQ7_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFR1134829.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            10669..13101
                     /locus_tag="ACHUQ7_001347"
     CDS             10669..13101
                     /locus_tag="ACHUQ7_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFR1134830.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIMLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVHVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13247..14062
                     /gene="ybiV"
                     /locus_tag="ACHUQ7_001348"
     CDS             13247..14062
                     /gene="ybiV"
                     /locus_tag="ACHUQ7_001348"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFR1134831.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            14214..15479
                     /locus_tag="ACHUQ7_001349"
     CDS             14214..15479
                     /locus_tag="ACHUQ7_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFR1134832.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLNRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(15720..17312)
                     /locus_tag="ACHUQ7_001350"
     CDS             complement(15720..17312)
                     /locus_tag="ACHUQ7_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFR1134833.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            17531..18451
                     /gene="ldtB"
                     /locus_tag="ACHUQ7_001351"
     CDS             17531..18451
                     /gene="ldtB"
                     /locus_tag="ACHUQ7_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR1134834.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(18509..19627)
                     /locus_tag="ACHUQ7_001352"
     CDS             complement(18509..19627)
                     /locus_tag="ACHUQ7_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFR1134835.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPMAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAAGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(19624..20091)
                     /gene="mntR"
                     /locus_tag="ACHUQ7_001353"
     CDS             complement(19624..20091)
                     /gene="mntR"
                     /locus_tag="ACHUQ7_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFR1134836.1"
                     /translation="MSRRAGTPIAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            20277..20405
                     /gene="mntS"
                     /locus_tag="ACHUQ7_001354"
     CDS             20277..20405
                     /gene="mntS"
                     /locus_tag="ACHUQ7_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFR1134837.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            20677..22260
                     /gene="opgE"
                     /locus_tag="ACHUQ7_001355"
     CDS             20677..22260
                     /gene="opgE"
                     /locus_tag="ACHUQ7_001355"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFR1134838.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(22308..22823)
                     /gene="ompX"
                     /locus_tag="ACHUQ7_001356"
     CDS             complement(22308..22823)
                     /gene="ompX"
                     /locus_tag="ACHUQ7_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFR1134839.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(22876..22941)
                     /locus_tag="ACHUQ7_001357"
     CDS             complement(22876..22941)
                     /locus_tag="ACHUQ7_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1134840.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            23176..24063
                     /gene="rhtA"
                     /locus_tag="ACHUQ7_001358"
     CDS             23176..24063
                     /gene="rhtA"
                     /locus_tag="ACHUQ7_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFR1134841.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVSAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVVILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            24362..24865
                     /gene="dps"
                     /locus_tag="ACHUQ7_001359"
                     /gene_synonym="pexB"
     CDS             24362..24865
                     /gene="dps"
                     /locus_tag="ACHUQ7_001359"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFR1134842.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            25269..26015
                     /gene="glnH"
                     /locus_tag="ACHUQ7_001360"
     CDS             25269..26015
                     /gene="glnH"
                     /locus_tag="ACHUQ7_001360"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFR1134843.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFSTEPK"
     gene            26154..26813
                     /gene="glnP"
                     /locus_tag="ACHUQ7_001361"
     CDS             26154..26813
                     /gene="glnP"
                     /locus_tag="ACHUQ7_001361"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFR1134844.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            26810..27532
                     /gene="glnQ"
                     /locus_tag="ACHUQ7_001362"
     CDS             26810..27532
                     /gene="glnQ"
                     /locus_tag="ACHUQ7_001362"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFR1134845.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALGNVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            27793..30017
                     /gene="ybiO"
                     /locus_tag="ACHUQ7_001363"
                     /pseudo
     CDS             27793..30017
                     /gene="ybiO"
                     /locus_tag="ACHUQ7_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
     gene            complement(30014..30940)
                     /gene="rlmF"
                     /locus_tag="ACHUQ7_001364"
     CDS             complement(30014..30940)
                     /gene="rlmF"
                     /locus_tag="ACHUQ7_001364"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFR1134846.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            31216..31476
                     /gene="mcbA"
                     /locus_tag="ACHUQ7_001365"
     CDS             31216..31476
                     /gene="mcbA"
                     /locus_tag="ACHUQ7_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFR1134847.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMLGTATIYK"
     gene            31741..>32041
                     /locus_tag="ACHUQ7_001366"
     CDS             31741..>32041
                     /locus_tag="ACHUQ7_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFR1134848.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPG"
BASE COUNT         7823 a         8584 c         7981 g         7653 t
ORIGIN      
        1 tcagggccga acgttcaata tctttaaaga aatagccaac gtttttctga ataagttcaa
       61 agcggctatc ggcacgtttt accgcgcgtt ccgataccag ttgatagtta atcatgaaaa
      121 aaagaccaat aaatgacagt gctgccaata aaaataaaat tatcagcctt atcttttcgt
      181 ataaactcag catgttaata catttaacaa aataactatc tgaacgctgt gccgctgcaa
      241 aaatagtccc ttgctgcggc tttttaacca aattacccga ttctacaaaa tcagcagaaa
      301 gttacaacac ttgcttaggg caataatttc ctgtgaggtg attacccttt caagcaatat
      361 tcaaacgtaa ctatccttta attttcggat ccagcgcatc gcgtaaacca tcgcccaaca
      421 aattgaacgc cagtacggtc agaaaaatag ccagggccgg aaaaacagcg acatgcggcg
      481 cgataaccat atccgctcgt gcctcattga gcattgctcc ccactctggt gtcggcggct
      541 gcgcaccgag gccgagaaat gacaggctgg cggcagagat aatcgaggtg ccaatgcgca
      601 tggtgaaaaa caccacgata gaagagacgg tcccaggcag gatatgacgc aataaaatgg
      661 tcatatcgct ggcaccaata ctgcgtgctg actcaataaa ggtttgctgt ttcaacacca
      721 gcgtgttgcc gcgcaccagg cgggcaaacg cggggatgga aaaaatggcg actgcgataa
      781 tcacgttagc aatgccgctt cccaacaccg caacaacagc gatcgccagt aaaatacccg
      841 ggaaggcaaa cagcacatcg caaatgcgca tgatcagccg atcccaccag ccttcataat
      901 atccagccaa caagcccagc aacgtcccta tcgccacacc gataaacacg gcaaacacgc
      961 ccgccgccag cgagatttgc gcaccaacca ggacacggct gaaaatgtcg cgccccagtg
     1021 aatcgacgcc aaaccagtgc tgcaaagaag gtccgttatt cagattgtca taatcaaaat
     1081 aattttcggc gtcataggga gcgatccagc gtgcaaaaat ggccaccaca atcaataaaa
     1141 taacgaataa tgcggcggtc atcgccatat gctgacggcg aaatcgtcgc cagaattcat
     1201 gccacggtgt acgtacctgg tcaggtttga ccagtggcat ggcgtttaac accgcctgac
     1261 gtcgccagtt aaatagtcgc atccttactt gtacctgata gccgggttaa tggcggcgta
     1321 aagcacatcc accactaagt tgataagaat aaattccagc gagaaaagca gaatttccgc
     1381 ctgaatcacc gggtaatcgc gcatttctac agagtcaacg agtaagcgtc caagtccggg
     1441 ccagttgaaa actttctcca ccacgataga accaccgagc aaaaagccaa actgtaagcc
     1501 catcatggtc actaccggga tcatcgcgtt gcgtagcccg tgtttgagga caacccaggt
     1561 ttcgctcacc cctttcgccc tcgcggtacg catataatct tcgcttaaaa cgtcgacaaa
     1621 tgacgcacgg gtaaagcgcg ccatcacggc ggccactgcc gcgccgagcg tcagggaggg
     1681 taaaatgtag tgctgccaac tgtctgctcc cacagtaggc agccagccca gttcaacgga
     1741 gaatacctga attaaaaaca tccccagcgc aaatgccgga aacgagatcc ccgacaccgc
     1801 aatggtcata ctcaatcgat ccggccaacg attacgccag acggcggcga taattcccgc
     1861 cgccatacca aatatcaccg cccagaccat actggttatg gtcagccata gcgttggcat
     1921 aaagcggctg gcaatctcat cggcaaccgg acgacgcgac accatcgaca ggccaaaatc
     1981 cccctgcaca gcattgctga tatagtgcca gaactggtga tacagcggct gatccaaccc
     2041 cagctgctga cgcaccagtt ctataacctg cgcatcagct tcgggcccgg caatcaatcg
     2101 cgccggatcg ccgggcagca tgtggacaaa taaaaacacc agcaccgaga cgataaacag
     2161 cgtcggaatc aaccccagta atcgtttgat aacgtaataa agcattccac tccctgcgtt
     2221 gcttattgca aatccgcgtc ttcaaagctg aagccggtat ctggcatgat ccaaaaaccg
     2281 gtcaggtttt tactgtgtgc cgacaccagt ttttctacca ccagcgggat ccacggcgat
     2341 tcttgccaga tgatatcctg cgccgcctta tataagcggg tcttttccgc cggatcattc
     2401 gttttcagtg cctgagccag gaagtcatcc acctgtttat tgctgtaaaa cgcggtatta
     2461 aacagcgtcg gtggccagtt ttgcgaggca aacagcggcg atagtgccca gtcggcttcg
     2521 ccggttgaag ccgaccagcc agtgtagaac atccgcacgc cactctcttt ttgcccttta
     2581 ccttcaactt ctgccgcccg ctgtccggca tccatcgcgg tcacctgggc tttaatcccg
     2641 acctgcgcta actgctgctg ggtaaattgc agcactttct gcgcggtgct gtggttatgt
     2701 gacgaccaca gcgtggtact gaaaccgttg ggatatcccg cctctttcag taattcgcgc
     2761 gctttcactg gatcgtaagg ccacggttta taactttgcg cgtaggcgat acttggcggt
     2821 accacaccag tagctggagt tgcatagccc gcaaaggcaa ctttcaccag cgccggacgg
     2881 ttaatggcgt aattcagcgc ctcacggacc ttcgggttat cgaacggctt ttgcgtcacg
     2941 ttcatactga tataacgctg cataattgac ggactggcca tcaactcgat atttttgttt
     3001 ttctccagca gtgtggcctg ctcgtaggga atggggaaag caaactgcgc ttcaccggtt
     3061 tgcagcattg ccgcgcgggt gttgttatcc gccaccggac gccaggttat gctgtccagt
     3121 ttgggcaatc ctggctgcca gtaacccgcg aattttttca ccttcacaaa atcggtctga
     3181 ttccaggtat ccagttcata cggtccggtt cccaccggat gaaaaccaat ctccttgcca
     3241 tatttttcca gcgctgtcgg tgaaatcatc gcggtcgccg gatgggcaag aatattaata
     3301 aacgctgaga acggctgttt gagggtaatc tttaccgttg tcggatcgat cgcctccgtt
     3361 ttggcaatat tcttatacag gttatagcgt ttaagatgat tcgccggatc gctggcccgg
     3421 tccagattcg cttttaccgc cactgcgttg aaatcggtgc catcctggaa tttaattcct
     3481 tcccgcaatt tcacggtgta agtaatgccg tcatcggaaa cggtataact ctccgccagc
     3541 acgtttttca gtttcatctc tttatccaga ccgaacagcc cctggtaaaa cgatttcgct
     3601 acggcctgag ataacgtgtc attcgcgtca tacggatcga gcgtggtgaa attcgatcct
     3661 accgccacca ccacatcttt ggcagcgaat gcacaagatg ccatcaacgc tgtcgcaatg
     3721 cccagcgcca ctaacccact acggtgtaca gctcttgcca ttttattctc cgcctgaatg
     3781 ttatctacgc atgaatgcgt attctgattg tggttgtgcg acgtaatgcc ccggcccgac
     3841 gcattgcaac gagacggctg ccacctcttc gccacgcaga tgaatattgc tgggaagatc
     3901 gtccgacagc agtacacgct gcggtcgttg tcgggacggt tcagcgaccg gaactgccgc
     3961 cagtaattta cgcgtataag gatgctgcgg gttttcgaag accgcgcacc gtgaaccaat
     4021 ttcaacaatt tgcccgagat acatcaccgc cacacgatga ctaatccgct ctaccacagc
     4081 catatcgtgg gagataaaca gatacgcaat gccgaaatca cgctggagat cgagcaacaa
     4141 gttgataatc tgcccgcgaa tagaaacatc cagcgccgaa acggcttcgt cggcaatgat
     4201 cacttttgga ttcaatgcca acgcgcgagc aatgcagatg cgctggcgct gaccgccgga
     4261 aaactcatgc gggtaacgcc aggcatgttc aggtaacagg cccacgcgct ccagcaacca
     4321 cgcaacgcgt gcagccgcgt ctttacctgg caataaaccg tgtacacgca gcggttcgat
     4381 aatcgaatca ccgatggtct gacgtgggtc cagcgaagcg taagggtcct gaaaaataaa
     4441 ctgaatatcc cggcgcaatg cctgaagttt gccaggtgac aaggtatcga ttcgctgacc
     4501 gttaaagata atttcgccgc cctgcgattc gaccaggcgc agcaacgccc gcccggtagt
     4561 ggatttaccg ctgccagact cgcccaccag cgatagcgtt tcgccaggcc agagatcaaa
     4621 actgactttc tcaacggcat gcacttcccg cgttacgcga ttcaacaaac cgctgcgcaa
     4681 agggaaacgg gtgacaagat tacgcacccg taaaacaggt tcgccatcca ccaccgtttt
     4741 ctgctcgatg gggggttcct gtttcgctgg atgttcaagc gatatcaatg ggaaacgtcg
     4801 gggataatct aaccctttca tcgcaccaag ttgcggaaca gcagctaaca gcgcacgggt
     4861 gtaaggatgt tgcggtgcat gaaaaatctg ttcgacggta cccgtttcca ccgcctcgcc
     4921 ctgatacatc accagtaccc gatcggcaat ctctgccacc acgcccatat cgtgagtgat
     4981 aaagataacg cccatcgaca tctctttttg caatactttg attaattgca ggatctgcgc
     5041 ctgaatagtg acatccagcg cggtggttgg ctcatcggca atcagcaccg ccgggcggca
     5101 tgacagcgcc atcgcaatca tcactcgctg gcgcatcccg ccagagagtt gatgcgaata
     5161 acgtgaaaga atggtttgtg cctccggaat gcgtacctga tccagcatcc gcttcgcctc
     5221 gaccatcgct tcttcacgac tggcgttctg atgcagacga attgattcgg caatctgttc
     5281 acccacagta aatactgggt tcagcgatgt catcggctcc tgaaatatca tcgccatatc
     5341 cgcaccgcgc acatggcgca tttgtgcagc gctctgctcg ctaagttcaa tcacatcacg
     5401 actgcgccgc cgcaacagca ttttatcgca ctgtactaaa ccgcccgcct gttccaacag
     5461 acgcatcaat gccagcgcag tcactgactt accggagccg gattcgccta caattgccag
     5521 cgtctcaccg cgttgcagac taaaagagag attgcggacc gcagctattt tctgctggtc
     5581 ctgcataaag gcaatattaa gattttcaac cgccagcaca ttaccggcat caagttcatc
     5641 actgtgtggc aacggtgtcc cctttttcac ggtagatacc ggtggttggc gtatcgcctg
     5701 cgtagcccca ggcgcgatac attccttcgg tgttaaacgg tagcgcgaca ttcccttcat
     5761 ggtcgatagc gattaagcca ccgctaccgc caagcgcagg gagtttttcc attactaccc
     5821 gctcgcaggc ttccgcgaga cttaatccgc cgtaatccat taacgcggcg atgtcatatg
     5881 ccgccagcgc gcggatgaag acttcgcctg tgccggtaca agaaaccgcc acactggcgt
     5941 tattggcgta gcatccggca cccactaagg gactatcgcc aactcgtccg ggtaatttat
     6001 tggtcattcc gcctgtggac gtggctgccg ccagattgcc gtctaaatcc aacgccacgg
     6061 cccccacggt gcccattttc tgtttttcat ccagtggcgc accgctatgg tcgaggactg
     6121 ttgccccttc ctcgcgcgct gccagtagtt gttcataacg caaaggcgtg gagaaaatct
     6181 ccggtgagac gcgctccatg ccatgagcaa acgcaaaatt ttctgcccct tcgccaatca
     6241 tcatcacatg cgggctttgc tccatcacca gccgggcggc aagaaccgga ttacgcagat
     6301 gactaacgcc cgccaccgca ccggctttca gggtgttacc atccatcaca caggcgtcca
     6361 gttcatgggt ttcatcacgc gtaaagacag cgccaattcc ggcgttaaac agtggacact
     6421 cttccagcag acgcaccgct tccgtcacca catccagcgc actttcgccg gcttccagca
     6481 ttttctgccc ggtttcaaca atggcagaca acgcctcgat gtagcgtaat tcctgttgca
     6541 gactcatctg cgcgcggcta attgcacctg cgccaccatg aattgcaatg actgctttgc
     6601 ccatagtatt cgtccattaa gggtggctcg ccacttttgc tataaatatc agaattttta
     6661 tgaattactc attcaatttt tgaatataga aagatggata tggcatgtaa aggcaggagc
     6721 caacgctacg cccgaggtgg catacagtct atggcgtttt tctgacataa taggcaaatt
     6781 cgattttgcc tccgcaggag tgttttcatg gaatttacca ccggattgat gtcgctcgac
     6841 accgcgctta atgagatgct ttctcgcgtt accccactga ccacccagga aacgctgcca
     6901 ctggttcagt gttttggtcg tattctggcg agcgatgtcg tttcgccact ggatgtcccg
     6961 gggtttgata actccgctat ggacggctac gcggtgcgtt tagccgatat tgcctccggg
     7021 caaccgctgc ccgttgccgg taaatccttt gccggtcagc cataccatgg tgaatggcct
     7081 gcgggtacct gcattcgtat tatgaccggt gcgccggtgc cggaaggctg cgaagcggtg
     7141 gtgatgcagg agcagactga acaaacggac aatggcgtgc gttttactgc tgaagtgcgt
     7201 agcgggcaaa atattcgccg tcgcggtgaa gatatctctg caggtgcggt tgttttcccg
     7261 gcgggaactc gcctgactac cgcagagctg ccagtaattg cttcactggg gattgccgaa
     7321 gttccggtga ttcgtaaagt gcgtgtagcg cttttttcta ccggtgatga actccagttg
     7381 cccggtcagc cgctgggcga cggccaaatc tacgacacca accgtctcgc cgtacacctg
     7441 atgttagaac agctgggttg cgaggtaatc aacttaggga ttatccgcga cgatccacat
     7501 gccctgcgcg ccgcatttat tgaagccgac agccaggcgg atgtagtgat cagttccggc
     7561 ggtgtttcag tgggtgaagc ggattacacc aaaatgattc ttgaagagct gggggagatc
     7621 gccttctgga agctggcgat taaaccaggt aaaccgttcg cgttcggtaa actcagcaat
     7681 agctggttct gcggcctgcc gggcaacccg gtttcagcga cgctgacctt ctatcaactg
     7741 gtacagcctt tgctggcaaa actaagcggc aacaccgcca gcggcctgcc cgcgcgccag
     7801 cgcgtacgca cagcgtcccg cctgaaaaaa acgccaggac gtcttgattt ccagcgcggc
     7861 gtgctgcaac gcaacgccga cggtgaactt gaagtgacga ccaccggaca tcagggttca
     7921 catatattta gctcctttag tctcggcaac tgctttatcg tgctggaacg cgatcgcggc
     7981 aatgtggacg tgcgcgaatg ggtggaagta gaaccgttta acgcgttgtt cggaggcctg
     8041 taatggcgga actcagcgat caggagatgc tgcgctacaa ccggcaaatc attctgcgcg
     8101 gttttgattt tgacggtcag gaggcgctaa aagattctcg tgtgctggta gtcggcctcg
     8161 gtggactcgg ctgcgcagct tcgcagtatc tggcaagcgc cggtgtcggc aacctgacgc
     8221 tgctcgactt cgacacggtt tcgctctcga atctgcaacg ccagacactg cacagtgatg
     8281 ccacggtcgg gcaaccgaag gtggaatccg cccgtgacgc cctgacgcgg atcaacccac
     8341 atatcgcgat tacgccagtc aatgcactgc tagatgacgc agaacttgca gcattgattg
     8401 ctgaacacga tctggtgctc gactgtacgg ataacgttgc ggtacgtaat caactgaacg
     8461 caggctgttt tgccgcgaag gtgccgctgg tttccggcgc ggcaattcgt atggaagggc
     8521 aaatcacggt cttcacttat caggacggcg aaccgtgcta tcgctgcctt agccgtttgt
     8581 ttggcgagaa cgcgttaacc tgcgtagaag caggcgtgat ggcaccgttg ataggcgtaa
     8641 ttggttcgtt gcaggcaatg gaagcgatca aactgctggc aggttatggc aaaccggcca
     8701 gcgggaaaat cgtcatgtac gatgcgatga cctgtcagtt tcgcgaaatg aaactgatgc
     8761 gcaatccggg gtgtgaggtg tgcgggcagt aattgccacc tccaagcctg ataagacgcg
     8821 acaagcgtcg catcaggcat ttgctccaga cgccagatgc ggcgtgaacg ccttatctgg
     8881 cctacggttc gggcacagat ttccaagcgc ggcaggcatc ggtgcactcc gccacgtcat
     8941 cagcaattta tgaggatgca gaacgtccgg ttaaatcgac gttctgccaa acgctccctg
     9001 ccagtcctgc tcaaacttcg tcacagcggc ttccaccgcc ggatagctaa tcatctgttg
     9061 tgccacatcc agtggcagag taattgattc acatccggcc agtaagcagt ccagcgcctg
     9121 acgcggggtt ttgaaactcg ctgccagcac tttcgcctgc ggcgcatgca ttttcaataa
     9181 ctggtgtaag tcggtcacag tctgaatgcc gctaccgccc tgagcatcaa tacgattaac
     9241 gtaaggcgca acatattccg cacctgccag cgccgacagc agcccttgtg ccgcgccata
     9301 taccgcggtt cccagcgtcg gaatcccttc cgcttttaac atcttaatcg ctgccagccc
     9361 ctcggctgtc accggaactt tcaccacgat atccgcaata atagaacgca gcttaagcgc
     9421 gtcattcacc atcccttcag cagtggtagc cattacctgg gcaaacagac gcccctgacc
     9481 gcccatcgct tcatgaagtt gcggaagcac aacatccagc ggttttttac ccgcggcgat
     9541 aatgcttggg ttagtggtca cacccgccag cggaaaaata cgtgacagcg ccttcaccgc
     9601 aacaacgtct gaagtatcca gatacagttc cataaccatc ctcaaaatgt actttttgtc
     9661 tgaatgtaga ctatcacggg aaaactctct gaacagttga cgcacatcaa tctaactttc
     9721 attcgaaagt attttaatgt ttgaacgaaa cagagaggca actatgattt tcaatattca
     9781 gcgctactcg acccatgatg gccccggtat ccgcacggtc gtatttctta aaggctgttc
     9841 gctgggctgc cgctggtgtc agaacccgga aagccgcgcc cgtacgcagg atctgctgta
     9901 tgacgcacga ctgtgtctgg aaggctgcga gctgtgcgct aaggccgcgc cggaagtgat
     9961 tgagcgcgcg ctgaatggtt tgcttattca tcgggaaaag ttaaccccgg agcatctgac
    10021 ggcgttaacc gactgctgtc cgacacaggc gttaaccgtg tgtggtgaag tgaaaagcgt
    10081 tgaggagatc atggcgaccg ttctgcgcga taaaccgttt tacgatcgca gcggcggcgg
    10141 cttaacgctt tcgggtggtg aacccttcat gcagccggaa atggcgacgg cgctgctgca
    10201 agcctgccac gaggcaggca ttcatactgc ggtagaaacc tgtctgcatg tgccgtggaa
    10261 atatatcgcc ccttctctgc cctatatcga tctgtttctt gccgatttaa aacacgttgc
    10321 cgacgcgccg tttaaacagt ggaccgacgg taacgccgcc agggtgctgg ataacctgaa
    10381 aaaactcgcc gcagcgggca aaaaaatcat tatccgcgtg ccgctgattc agggctttaa
    10441 tgccgacgaa acctctgtaa aagccattac cgattttgcc gccgacgagc tgcacgttgg
    10501 cgaaattcat tttctgccct accacacgct gggcatcaac aaatatcact tacttaatct
    10561 gccctatgac gccccggaaa aaccgcttga tgcgccagag ctgctcgatt ttgcccagca
    10621 gtatgcctgc cagaaagggt taaccgcgac cttacgagga taacaaccat gaccacactg
    10681 aaactggaca cgctcagcga ccgcattaaa gcgcacaaaa atgcgctggt gcatattgtg
    10741 aagccgccag tctgtaccga gcgcgcgcag cactataccg agatgtatca acaacatctc
    10801 gataagccga tcccggtacg tcgcgcgctg gcactggcgc atcacctggc gaatcgcacc
    10861 atctggatca aacacgatga gttgatcatt ggcaaccagg caagcgaagt tcgcgccgcg
    10921 ccgatcttcc cggaatatac tgtctcgtgg atcgaaaaag agattgatga tctggcagat
    10981 cgtcccggtg ctggctttgc ggtgagcgaa gagaacaaac gtgttctgca tgaagtgtgc
    11041 ccgtggtggc gcggtcagac ggtacaggat cgctgctatg gcatgtttac cgatgagcaa
    11101 aaaggtctgc tggcgaccgg catcattaaa gcggaaggca atatgacctc cggcgatgcg
    11161 cacctggcgg tcaatttccc gctgctgctg gaaaaagggc ttgatggtct gcgtgagaaa
    11221 gtagcggaac gtcgctcgcg catcaatctg acggtgctgg aagatttgca cggcgaacag
    11281 ttcctgaaag cgattgatat catgctggtg gcagtcagtg aacacattga acgtttcgct
    11341 gccctggcgc gtgcaatggc cgcgaccgaa acccgcgaaa gccgtcgcga tgaactgctg
    11401 gcgatggcag aaaactgcga tcttatcgcc caccagccgc cgcagacttt ctggcaggcg
    11461 ctgcaactgt gttacttcat ccagttgatt ttgcagattg aatctaacgg tcactcggtg
    11521 tcgtttggtc gtatggatca gtatctctac ccgtactatc gccgcgacgt tgaactgaac
    11581 cagacgctgg atcgcgaaca cgccatcgaa ttgctgcata gctgctggtt gaaattgctg
    11641 gaagtgaaca aaatccgctc cggctcccac tcaaaagcct ctgcgggaag tccgctgtat
    11701 cagaacgtca ccattggcgg acaaaatctg gttgatggtc aaccaatgga cgcggtgaat
    11761 ccactctctt acgcgatcct cgaatcctgc ggtcgcctgc gttccactca gcctaacctc
    11821 agcgtgcgtt accatgcagg aatgagtaac gatttcctcg acgcctgcgt acatgtgatc
    11881 cgctgcggct tcgggatgcc ggcattcaac aacgacgaaa tcgtgatccc ggaatttatt
    11941 aaactcggta ttgaaccgca ggacgcttat gactacgcag cgattggttg tatagaaacc
    12001 gccgtcggtg gcaaatgggg ctatcgctgt accggcatga gctttatcaa cttcgcccgc
    12061 gtgatgctgg cggcgctgga aggcgggcgt gatgccacca gcggcaaagt gttcctgcca
    12121 caagaaaaag cgttgtcggc aggtaacttc aacaacttcg atgaagtgat ggacgcgtgg
    12181 gatacgcaaa tccgttacta cacgcgtaaa tcaatcgaaa tcgaatatgt cgtcgacacc
    12241 atgctggaag agaacgtgca cgatattctc tgctcggcgc tggtggatga ctgtattgag
    12301 cgagcgaaaa gtatcaagca aggcggcgcg aaatatgact gggtttctgg cctgcaggtc
    12361 ggtattgcca acctcggcaa cagcctggcg gcagtgaaga aactggtgtt tgaacagggc
    12421 gcgattggtc agcaacagct ggctgccgca ctggcggatg acttcgatgg cctgactcat
    12481 gagcaactgc gtcagcggct gattaacggt gcgccgaagt acggcaacga cgatgatacc
    12541 gtcgatacgc tgctggctcg cgcttatcag acctatatcg acgaactgaa acagtaccat
    12601 aacccacgct acggtcgtgg tccggttggc ggcaactatt acgcgggtac gtcgtctatc
    12661 tccgctaacg taccgtttgg cgcgcaaact atggcaacgc cggacgggcg taaagcccac
    12721 accccgctgg cagaaggcgc tagtccggcc tctggtactg accatctcgg ccctactgcg
    12781 gtcattggct cagtgggtaa actgcctacg gcagcgattc tcggcggcgt gttgctcaac
    12841 cagaaactga atccggcaac gctggagaac gaatctgaca agcagaaact gatgatcctg
    12901 ctgcgtacct tctttgaagt gcataaaggc tggcatattc agtacaacat cgtttcccgc
    12961 gaaacgctgc tggaggcgaa aaaacatccg gatcagtacc gcgatctggt agtgcgtgtc
    13021 gcgggttatt ccgccttctt caccgcgctc tctccagacg ctcaggacga tatcatcgcc
    13081 cgtactgaac atatgctgta atctcttcgg cccggctgcg gcgtcgccgg gctaaatcat
    13141 tcacatcatc aatttcatcc ttactttcat tcgaaatata atttgtgctc tgcgtcacat
    13201 tgttattaca ctgttacggt ttcattttcg agccaggagc acacctatga gcgtaaaagt
    13261 tatcgtcaca gatatggacg gtacttttct taacgacgcc aaaacgtaca accaaccacg
    13321 ttttatggcg caatatcagg aactgaaaaa gcgcggcatt aagttcgttg tcgccagcgg
    13381 taatcagtat taccagctta tttcgttctt tcctgagcta aaggatgaga tctcttttgt
    13441 cgcggaaaac ggcgcactgg tttacgaaca tggcaagcaa ctgttccacg gcgaactgac
    13501 ccgacatgaa tcgcggattg ttattggcga gttgctaaaa gataagcaac tcaattttgt
    13561 cgcctgcggt ctgcaaagtg catatgtcag cgaaaatgcc cccgaagcat ttgtcgcact
    13621 gatggcaaaa cactaccatc gcctgaaacc tgtaaaagat tatcaggaga ttgacgacgt
    13681 actgttcaag ttttcgctca acctgccgga tgaacaaatc ccgttagtga tcgacaaact
    13741 acacatagcg ctggatggca ttatgaaacc cgtcaccagt ggttttggct ttatcgacct
    13801 gattattccc ggtctacata aagcaaacgg tatttcgcgg ttactgaaac gctgggatct
    13861 gtcaccgcaa aatgtggtag cgattggcga cagcggtaac gatgcggaga tgctgaaaat
    13921 ggcgcgttat tcctttgcga tgggcaatgc tgcggaaaac attaaacaaa tcgcccgtta
    13981 cgctaccgat gataataatc atgaaggcgc gctgaatgtg attcaggctg tgctggataa
    14041 cacatcccct tttaacagct gacctctcta ccggagccat gcgctccggt tttcccttct
    14101 ttttacatcc tgtacatcac atcaaatcat taaacttgca ttaacttatt tataacttgt
    14161 attgtcacat gcaaattagc atagtttcga atgaaagtta atgcaaggtg gttatggctt
    14221 ctacttttac cagcgacaca ttgcctgccg atcacaaagc agctatccgt cagatgaagc
    14281 acgcgctgcg ggcgcagctt ggcgatgtcc agcagatctt taatcagcta agcgatgaca
    14341 ttgccacgcg agtggctgaa atcaacgcac tcaaagcaca gggcgatgcc gtctggccgg
    14401 tgctgtctta tgccgatatc aaagcaggtc atgttactgc agagcagcgc gaacagatta
    14461 aacgtcgcgg ttgtgcggtg ataaaaggcc atttcccccg cgaacaagcg ctaggctggg
    14521 atcagtcgat gctggactat ctgaaccgca accgctttga cgaggtctac aaaggccccg
    14581 gcgataattt cttcgggacg ctcagcgctt cacgtcccga gatttacccc atctactggt
    14641 cgcaggcgca aatgcaggcc cgccagagtg aagaaatggc gaatgcgcag tcgtttctca
    14701 atcgtctgtg gacatttgaa agtgatggaa agcaatggtt taacccggat gtgagcgtca
    14761 tctaccctga ccgtatccgc cgccttccgc ccggaacgac ctccaaaggt cttggagcgc
    14821 ataccgactc cggggcgctg gaacgctggc tgcttccagc gtatcagcac gttttcgcca
    14881 acgtctttaa tggcaatctg gcgaaatacg atccctggca tgcggcacat cgtacggaag
    14941 ttgaagagta cacggtggac aacaccacca aatgttccgt gtttcggaca ttccagggct
    15001 ggactgcgct ctcggatatg ctgcctggtc aggggttgct gcacgtcgtg cccattcctg
    15061 aagccatggc gtacgtactg ttacgtccgc tgcttgatga tgtgccggag gatgaactgt
    15121 gcggcgtagc gcccggaaga gtgttgccgg tatcagagca atggcatcca ctgttaattg
    15181 aggcgttaac cagcattcca aaactcgagg ccggagactc cgtctggtgg cactgcgacg
    15241 tcatccattc cgttgccccc gttgaaaatc aacagggttg gggcaacgtg atgtacattc
    15301 ctgcggcacc gatgtgcgag aaaaatcttg cctacgcgca caaggtgaag gccgcactgg
    15361 aaaaaggcgc atcgccgggc gacttcccgc gcgaggacta tgaaacaaac tgggaaggac
    15421 gctttacgct tgccgacctc aacattcacg gtaagcgagc gttgggcatg gatgtttgag
    15481 agttaatgga agtgaattga tgtgggatta agaaaatcga ttcgacgtta ctcagattgc
    15541 tgacaagtgc gctttcttaa tgcctgatgc ggcgttaacg ccttatccgg cctacaaagt
    15601 cctgcagatt caatatatta caggtactgc gtaggcctga taagcgtagc gcatcaggct
    15661 attttgcatt tgtcatcagc cgtcgtcacc ccgacagtat cggggtgacg gataatcact
    15721 tactcgatcc ctttactacg caggtaatct tcgtaattac cgctaaagtc gatcacgcgt
    15781 tccggggtga tttccagaat gcgggtcgcc agggagctta cgaactcacg gtcgtgtgaa
    15841 acaaagatca gcgtgccctg atacagttcc agcgccatgt tcagtgactc gatggattcc
    15901 atatccaggt ggttggtcgg ttcgtccatg atcagaatgt tcggcttctg catcattaac
    15961 ttaccaaaca gcatccgccc tttttcccca ccggaaagca ctttagctgg ctttttgatg
    16021 tcgtcctggc tgaacagcaa acgaccgaga atgctgcgta ccgcctgctc gtcatcgcct
    16081 tcctgcttcc actgactcat ccattcgaac acggtcagat cattttcaaa ctcatattcg
    16141 tggtcctggg cgtagtaacc aatgcgcgcg ttttcagacc attttacggt gccgctgtcc
    16201 ggttgcagat cgcccaccag cgttttcagc agcgttgatt taccgacgcc gttggtaccc
    16261 agtaccgcaa gtttttcacc gacctccagc agcaggttga gatttttaaa cagtggaccg
    16321 ttatcaaacc ctttggtcag accttccact tccagcgcgt tacggaacag tttcttatcc
    16381 tgttcaaaac ggataaacgg gttctgacgg ctggaggctt tcacctcttc cagtttgatt
    16441 ttatcaatct ggcgcgcgcg cgaagttgcc tggcgagatt tcgaggcgtt ggcgctaaag
    16501 cggctaacga aagattgcaa ctcagcaatc tgcgctttct tcttggcgtt atcggccagc
    16561 agacgttcac gcgcttgggt cgccgccgtc atgtactcat cgtagttacc cggataaacg
    16621 cgcagctcgc cgtaatccag atccgccatg tgggtacaga ccatgttaag gaagtgacgg
    16681 tcgtgcgaga tgatgatcat ggtgctgtca cgctcgttca gcacctgttc cagccagcga
    16741 atggtgtcga tgtcgaggtt gttggtcggt tcgtcgagca ggagaatatc cggctcagca
    16801 aacagcgcct gcgccagaag cacacgcagc ttccagccag gagcaacttc actcatcggg
    16861 ccgtagtgct gttccactgg aattcccacg ccaagcaaca gttcaccggc gcgagcttcc
    16921 gcagagtaac cgtccatttc gccgtatttc acttccagat cggccacttt atagccgtct
    16981 tcttcactca tttccggcaa agcatagatg cggtcgcgct cctgcttcac ttcccacaac
    17041 tctttatgcc ccatgatcac cgtatccagc acagtgaact cttcaaaggc aaactgatcc
    17101 tgacgcagct taccaatgcg ctcgttggga tcgagggaaa cgttacccag cgtcggctca
    17161 aggtcgccgc cgagaatctt cataaaggtg gatttaccac taccgttcgc gccaatcagg
    17221 ccgtaacggt tgccgccgcc gaatttgacg gaaatgtttt caaacaacgg cttactgccg
    17281 aactgcatgg tgacgttact ggaaactaac acaggcgtat cctgaaaaga gatatgacaa
    17341 accgcgcatt atgccataag tctgaaagaa gatcctgctc ctcgcttaag gtctacactc
    17401 aagccaaagg caaaaaatgt gatttcgtac acatctgatt tcactgtgag ctggaatgaa
    17461 cttataatgc gcttccaata ctctaatatt ctcaacccaa tggcctgcca ggcacaaaat
    17521 ctcgcttaac atgaatatga aattgaaaac attattcgca gcggccttcg ctgttgtcgg
    17581 cttttgcagt accgcctctg cggtaactta tcctctgcca accgacggga gtcgcctggt
    17641 tggtcagaat caggtgatca ccattcctga aggtaacact cagccgctgg agtattttgc
    17701 cgcggagtac cagatggggc tttccaatat gatggaagcg aacccgggtg tggatacctt
    17761 cctgccgaaa ggcggtactg tactgaacat tccgcagcag ctgatcctgc cggataccgt
    17821 tcatgaaggc atcgtcatta acagtgcaga gatgcgtctg tattactatc cgaaagggac
    17881 caacaccgtt atcgtgctgc cgatcggcat tggtcagtta ggcaaagata cgcctatcaa
    17941 ctggaccacc aaagttgagc ataagaaagc aggcccgacc tggacgccga ccgccaaaat
    18001 gcacgcagag taccgcgctg cgggcgaacc gctaccggct gtcgttccgg caggtccgga
    18061 taacccgatg ggtctgtatg cactctacat cggtcgcctg tatgctatcc atggcaccaa
    18121 cgccaacttc ggtatcggcc tgcgtgtaag tcatggttgt gtgcgtctgc gtaacgaaga
    18181 catcaaattc ctgttcgaga aagtaccggt cggtacccgc gtacagttta ttgatgagcc
    18241 ggtaaaagcg accaccgagc cagacggcag ccgttatatt gaagtccata acccgctgtc
    18301 taccaccgaa gcccagtttg aaggtcagga aatcgtgcca attaccctga cgaagagcgt
    18361 gcagacagtg accggtcagc cagatgttga ccaggttgtt cttgatgaag cgatcaaaaa
    18421 tcgttccggg atgccggttc gcctgaatta atcgtcaaaa cgtaaaacaa aggcggactc
    18481 ataatccgcc ttttttattt gccagacctt accaggccgg gatcataact aacaaaacgt
    18541 atcccaccag cgccgcccac aacagcatcg gtattgaata gagatggaaa cgccaccaga
    18601 tgcggcgatc gttcgccata cgtagcgcaa tcaaatttgc cagcgatcct ggtaataaac
    18661 caaagccccc cacgtttacc gcccatgcca gtaataaaga cggaggtaca taattgagca
    18721 gcaaaatggt actcggcacg ttactgatca cctgcgataa accgactgcc gttaaccata
    18781 acccgggttc agataaatga ctcacgttac ccaacacgcc ttgcaacgct ggaagctggg
    18841 tcagtaaatg gacgtcgata aacatcgcca taaacaccag cagcaacgtc cagtccacac
    18901 tcaacaccac gcgacgcgcc agcagcacaa agcccgccgc cacaatcacc agtccccaca
    18961 gctcttgttt gaactccagc gccgtcagaa agacgatata cagccccaga caattccaca
    19021 ccagccgcgg tttccactcc ggtgtttgca cccccgtatg gtattgcagc gcctttccag
    19081 ggaaacaaca ccagcacaac agcaggagtg tcagcatcat tgcgccagcc atcggtgcca
    19141 tttgggcaat aaatccggca aacgaaagac cagaacgtcc ccagataaga atattttgcg
    19201 ggttgccaat tggcgtcagt agcgaaccag cgttgactgc cagcgcctca aaaataatca
    19261 gccgattaac cgggatctca cacagtcttt ttagcgtgat agtcagcgga acaacaataa
    19321 acagcgcgac atcgttggtc agaaaggtag aaagcagcgc cgccgccagc accataaaca
    19381 tcgccagccg acgctccgta gcaaagcggc gcaccatttt gcgccccagc acatcaaaat
    19441 aaccgcttaa ctccacacct ttggtcagca gcatcaggcc gcttaaggtg atgatggtgt
    19501 gccagtcgat agcagcaggc caggatttcg gtgcaaaggg cacgaaaaag cttaatccga
    19561 taccaacaag aattaataac tgaaaaaaac gatcgccttg cagcgtgcgt aaaaaaggca
    19621 ggctcatttg gcaccgtgtt tctgggtaaa caaacgaaaa gcgtcgagcg tctcttcact
    19681 aacatggtgc tccatgcctt ccgcgtcgcg acgggcgatt tccggactga cgcccaacac
    19741 caacaagaaa ttttcgacta tctgatgtcg ctcgcggctt tcctgcgcca gcttctctcc
    19801 ttctgccgtt aaaaacacgc cacgccaggg gatcatttca atcagcccca tggttgccag
    19861 ccgcttaagc attttagcca ccgtcggttg cgaaactccc agacgagcag ccatgtccac
    19921 ctgacgagct tcccccactt ccctgatcaa gtcagaaatc agctcaacgt aatcatcaat
    19981 aagctcgcgc cgatgcgcct ctctgacctg gcggaaccct tcaacgtgct cttccacgtt
    20041 cactaactgc gtcacttttt ttgctattgg cgtacctgcg cgacgactca ttgtgcttcc
    20101 tcattatggt gacgcataca tagcgttctc atttcgtgcg cacattgtaa accagagttg
    20161 cgaaggtaca aaaaattaac gttttagcaa tagctatata atatagcctg tgctatatct
    20221 gtatgtaatg caatcatccc tcaaggatcg acgggattag caagtcagga ggtcttatga
    20281 atgagttcaa gaggtgtatg cgcgtgttta gtcattctcc ctttaaagta cggttaatgc
    20341 tgctctctat gttgtgcgat atggtcaaca acaaaccgca gcaagataaa ccttcagata
    20401 aatagcggcg tcgcggtacg ccgcttcact cctgctttta tgcaggcata acgcgttttg
    20461 gtctgaaaaa aaccactttt tgtcggattt gcaatcccct tcgcaaaaga tttgttcgtc
    20521 agtagttgac ctgaacggcg gctcgctcta tcttcttgca gccctgcgta tattgcggct
    20581 cgcggatgcg gacccctttc ccctcttcac gcactcttgc aggtattgac ccttgacgcc
    20641 agggtaagca catggcgttt gttacgatag tggcatatga atttaaccct caaagaatcg
    20701 cttgttaccc gtagccgggt atttagcccg tggactgcgt tctacttttt acagtcgcta
    20761 ttaattaacc tcggcttagg ttaccccttc agtttgctct acaccgctgc gtttacggct
    20821 attttgcttt tgctatggcg aacattgcct cgcgtacaaa aagttctggt cggtgtcagt
    20881 tcgctggtgg cggcttgtta tttccctttt gctcaggcct acggcgcgcc taatttcaat
    20941 acattgctgg cattgcactc caccaatatg gaagagtcga ccgaaatcct gacgattttt
    21001 ccgtggtaca gctacctggt cggcttattt atttttgcgc tcggcgtaat agcaatcagg
    21061 cgaaaaaaag agaatgaaaa agcgcgctgg aataccttcg acagcctgtg tctggtattc
    21121 agtgtggcga cattttttgt tgctcccgtg caaaacctgg cctggggtgg cgtatttaaa
    21181 ctgaaagata ctggctatcc ggtatttcgt tttgctaagg atgtcatcgt caataataac
    21241 gaggtgattg aagagcaaga acggatggca aaactttccg gaatgaaaga tacctggacg
    21301 gtcactgccg ttaagccgaa gtatcagacc tatgtggtag tgatcggtga aagcgcgcgt
    21361 cgcgatgccc tcggtgcctt tggcggtcac tgggacaata ccccgtttgc cagcagcgtt
    21421 aacggtttga tatttgctga ctacattgct gccagtggct ccacgcagaa atcgcttggc
    21481 ttaacgctca atcgcgtagt cgatggcaaa ccacagtttc aggataactt tgtcaccctg
    21541 gcaaatcgcg cgggcttcca gacctggtgg ttttccaacc agggccaaat cggcgaatac
    21601 gatactgcta tcgccagcat cgccaaacgt gcagatgaag tgtacttcct gaaagaaggt
    21661 aattttgaag cagataaaaa cacgaaagac gaagcgttac tggatatgac cgctcaagtg
    21721 ctggcgcaag agcactcgca accgcagctg attgttctgc atctgatggg ctcgcatccg
    21781 caggcctgcg acaggacaca aggcaaatac gaaacctttg tgcaatcgaa agaaacgtcg
    21841 tgctatctct ataccatgac gcaaacggac gatttactgc gcaagctgta cgatcagtta
    21901 cgcaacagcg gcagcagctt ctctctggtt tacttttctg accacggtct ggcctttaaa
    21961 gagcgcggca aagacgtgca ataccttgcc catgatgata aatatcagca aaatttccag
    22021 gtgcctttta tggtcatttc cagcggcgat aaagcgcatc gggtgattaa agcccgccgc
    22081 tcagccaatg acttcttagg ctttttctcg cagtggacgg gaattaaagc gaaggaaata
    22141 aatatcaaat acccgtttat atctgagaag aaagccgggc cgatatacat caccaacttc
    22201 cagttacaga aggtggatta caaccatctc ggaaccgata ttttcgaccc gaaaccttaa
    22261 aaacaaaaat ccgccccgag aggcggattt ttatatcacc aaagtgatta gaagcggtaa
    22321 ccaacaccgg caatccaggt gcctacgtca acgctacgaa tacggctctg ctcgtaagag
    22381 aagtccagag caacgttttc catcgggttg aactgcagac cagcaccgta ggagaaaccg
    22441 tagtcgctgg tgtcgtgttt gtaggtcggg tattcagtgg tctggaattt accataaccc
    22501 acacccacta caccgtagat gcttgcccag tcgttaatgc ggtaagccgg accagcagtg
    22561 atgccgtagt actggttttt gttgtagtca ccagagcttg cagtacggct tttctcggtg
    22621 taagtgaaag aaccgatcac acccagcggg ctgttgtctt cttcatagcg gtatttcagg
    22681 ttgaaaccgc ccattttgtt catttggccc tgagcgtcgc tctgtgcgta accgccagtt
    22741 acggtagaag tcgcagctac ggaagtacct gcggtgaaag ccagaactgc ggccagtgct
    22801 gaaagacatg caattttttt cataaccacc tcaaatgtga ttcaaataag tcctaagttt
    22861 taaatatatc aaaaattaat gggaaactct tcgcgatttg tgatgtctaa cgggccattt
    22921 catgtaacag aacgtttcca tacaccgcta tccatctaaa tttaaatcac tttttcagag
    22981 aactgcgtaa gtattacgca tgttttccct gtcattcatc cagattattc ctaatcacca
    23041 gactaatgat tccatcaatc ctggcgcatt ttagtcaaaa cgggggaaaa ttttttcaac
    23101 aaatgctcaa ccagcattgg gtatatccag tacactccac gctttactta agtctagata
    23161 tttgtgggag aaaggatgcc tggttcatta cgtaaaatgc cggtctggtt accaatagtc
    23221 atattgctcg ttgccatggc gtctattcag ggtggagcct cgttagctaa gtcacttttt
    23281 cctctggtga gcgcaccggg tgtcactgcg ctgcgtctgg cattaggaac gctgatcctc
    23341 atcgcgttct ttaagccatg gcgactgcgc tttgccaaag agcaacggtt accgctgttg
    23401 ttttacggcg tttcgctggg tgggatgaat tatctttttt atctttctat tcagacagta
    23461 ccgctgggta ttgcggtggc gctggagttc accggaccac tggcggtggc gctgttctct
    23521 tctcgtcgcc cggtagattt cgtctgggtt gtgctggcgg ttcttggtct gtggttcctg
    23581 ctaccgctgg ggcaagacgt ttcccatgtc gatttaaccg gctgtgcgct ggcactgggg
    23641 gccggggctt gttgggctat ttacatttta agtgggcaac gcgcaggagc ggaacatggc
    23701 cctgcgacgg tagcaattgg ttcgttgatt gcagcgttaa ttttcgtgcc aattggagcg
    23761 cttcaggctg gtgaagcact ctggcactgg tcggttattc cattgggtct ggctgtcgtt
    23821 attctctcga ccgctctgcc ttattcgctg gaaatgattg ccctcacccg tttgccaaca
    23881 cggacatttg gtacgctgat gagcatggaa ccggcgctgg ctgccgtttc cgggatgatt
    23941 ttcctcggag aaacactgac acccatacag ctgctggcgc tcggcgctat catcgccgct
    24001 tcaatggggt ctacgctgac agtacgcaaa gagagcaaaa taaaagaatt agacattaat
    24061 taaatttaca tttctgtatg gttatgcata accatgcaga atttctcgct acttttcctc
    24121 tacaccgtat ttatatatcg aattatgcaa aagcatattt attccgaaaa ttcctggcga
    24181 gcagataaat aagaattgtt cttatcaata tatctaactc attgaatctt tattagtttt
    24241 gtttttcatg cttgttacca ctattagtgt gataggaaca gccagaatag cggaacacat
    24301 agccggtgct atacttagtc tcgttaatta ctgggacata acatcaagag gatatgaaat
    24361 tatgagtacc gctaaattag ttaaatcaaa agcgaccaat ctgctttata cccgcaacga
    24421 tgtctccgac agcgagaaaa aagcaacagt agagttgctg aatcgccagg ttatccagtt
    24481 tattgatctt tctttgatta ccaaacaagc gcactggaac atgcgcggcg ctaacttcat
    24541 tgccgtacat gaaatgctgg atggcttccg caccgcactg atcgatcatc tggataccat
    24601 ggcagaacgt gccgtgcagc tgggcggtgt agctctgggg accactcaag ttatcaacag
    24661 caaaaccccg ctgaaaagtt acccgctgga catccacaac gttcaggatc acctgaaaga
    24721 actggctgac cgttacgcaa tcgtcgctaa tgacgtacgc aaagcgattg gcgaagcgaa
    24781 agatgacgac accgcagata tcctgaccgc cgcgtctcgc gacctggata aattcctgtg
    24841 gtttatcgag tctaacatcg aataaatcca tcgctgatgg tgcagaactt tagtacccga
    24901 taaaagcggc ttcctgacag gaggccgttt tgttttgcag cccacctcaa cgcacttatt
    24961 tagtgcatcc atctgctatc cccagctgat taagtaaatt ttttgtatcc acatcatcac
    25021 acaatcgtta cataaagatt gttttttcat caggttttac gctaaataat cactgtgttg
    25081 agtgcacaat tttagcgcac cagattggtg ccccagaatg gtgcatcttc agggtattgc
    25141 cctataaatc gtgcatcacg tttttgccgc atctcgaaaa atcaatgagt tgcaaaattg
    25201 gcacgatttt ttcatatatg tgaatgtcac gcaggggatc gtcccgtgga tagaaaaaag
    25261 gaaatgctat gaagtctgta ttaaaagttt cactggctgc actgaccctg gcttttgcgg
    25321 tttcttctca tgccgcggat aaaaaattag ttgtcgcgac ggataccgcc ttcgttccgt
    25381 ttgaatttaa acagggcgat aaatatgtgg gctttgacgt tgatctgtgg gctgccatcg
    25441 ctaaagagct gaagctggat tacgaactga agccgatgga tttcagtggg atcattccgg
    25501 cactgcaaac caaaaacgtc gatctggcgc tggcgggcat taccatcacc gacgagcgta
    25561 aaaaagcgat cgatttctct gacggctact acaaaagcgg cctgttagtg atggtgaaag
    25621 ctaacaataa cgatgtgaaa agcgtgaaag atctcgacgg gaaagtggtt gctgtgaaga
    25681 gcggtactgg ctccgttgat tacgcgaaag caaacatcaa aactaaagat ctgcgtcagt
    25741 tcccgaacat cgataacgcc tatatggaac tgggcaccaa ccgcgcagac gccgttctgc
    25801 acgatacgcc aaacattctg tacttcatca aaaccgccgg taacggtcag ttcaaagcgg
    25861 taggtgactc tctggaagcg cagcaatacg gtattgcgtt cccgaaaggt agcgacgagc
    25921 tgcgtgacaa agtcaacggc gcgttgaaaa ccctgcgcga gaacggaact tacaacgaaa
    25981 tctacaaaaa atggttcagt actgaaccga aataataacg ctacacctgt aaagcgcact
    26041 ggcagttccc tctcccccct ggggagagga ttagggtgag gggcgcaaac ccgctccggg
    26101 gccattaatt accctgaatt tgattattta caccacggta acaggaacaa catatgcagt
    26161 ttgactggag tgccatctgg cctgccattc cgcttctgat tgaaggtgcc aaaatgaccc
    26221 tgtggatttc ggtcctcggt ctggctggcg gtctggtaat cggattgctg gcaggttttg
    26281 cacgcacctt cggtggttgg atagccaacc acgtcgcgct ggtctttatt gaagtgatcc
    26341 gcggcacacc tatcgtcgtc caggtgatgt ttatctattt cgccctgccg atggcgttta
    26401 acgacttacg catcgaccca tttactgcgg cggtggtcac catcatgatc aactccggcg
    26461 cgtacattgc ggaaatcacg cgtggtgcgg tgctgtctat ccacaaaggt tttcgtgaag
    26521 caggactggc gctcggtctt tcacgttggg aaaccattcg ctacgtcatt ttaccgctgg
    26581 cactgcgtcg tatgctgccg ccgctgggta accagtggat catcagcatt aaagacacct
    26641 cgctgtttat tgttatcggc gtggcggaac tgacccgtca ggggcaagaa attattgccg
    26701 gtaacttccg cgcccttgag atctggagcg ccgtggcggt gttctatctg attattaccc
    26761 tggtgctgag ctttattctg cgtcgtctgg aaagaaggat gaaaatcctg tgattgaatt
    26821 taaaaacgtc tccaagcact ttggcccaac ccaggtgctg cacaatatcg atttgaacat
    26881 tgcccagggc gaagtcgtgg tgattatcgg gccgtccggt tccggtaaat cgaccctgct
    26941 gcgctgcatc aacaaactgg aagaaatcac ctccggcgat ctgattgtcg atggcctgaa
    27001 ggtcaacgat ccgaaagttg acgagcgcct gattcgccag gaagcaggta tggtgttcca
    27061 gcagttttac ctcttcccgc atctgacggc gctgggaaac gtcatgtttg gtccgctacg
    27121 cgtgcgtggc gcgaacaaag aagaggcgga aaaactggcg cgtgagctgc tggcgaaagt
    27181 cggtctggca gaacgtgcac atcactaccc ttccgaactt tctggtggtc aacagcagcg
    27241 tgtggcgatt gcccgcgcgc tggcggtgaa gccgaaaatg atgctgtttg atgaaccgac
    27301 ttccgctctt gacccggaac tgcgccatga agtgctgaag gttatgcagg atctggctga
    27361 agaagggatg acgatggtga tcgtgaccca cgaaatcggt tttgccgaga aagtagcttc
    27421 gcggctgatc tttatcgaca aaggccggat tgcggaagat ggcaatccgc aggtgttgat
    27481 caagaacccg ccgagccagc gcttgcagga atttttgcag cacgtctctt aataagacac
    27541 attgcctgat gcgctacgct tatcaggcct acaggatatc tggcaactta ttaaaattgc
    27601 atgaacttgt aggacggata aggcgttcac gccgcatccg gcaaaaaagc ccgcacgttg
    27661 tcagcaacct gcttaatatc ccttcctccc tttcacccga aagggaggca caccagattc
    27721 ctctcattta aaatcgcccc tcccccagca tctatactta tctttttgct ctgttttctc
    27781 actggaggag tcatgcggtg gatcctgttc atcctcttct gcctgctggg cgcacctgcc
    27841 cacgcggtat ccatacccgg cgttacaacc acaacgacaa cggactcaac gactgaaccg
    27901 gccccggaac cggatatcga acaaaaaaag cggcctatgg cgcactggcg gatgtgctgg
    27961 ataacgacac ctcgcgtaaa gagttgatcg accagttgcg tactgtcgcc gcgacgcccc
    28021 ctgcggaacc ggtcccgaag atcgtgccgc cgacgctggt tgaagagcaa accgtgctgc
    28081 aaaaggtcac cgaagtcagc cgccattatg gtgaagccct ttccgcccgc ttcgggcaac
    28141 tttatcgcaa tatcaccggc tccccgcata agccatttaa tccacaaacc ttcagcaatg
    28201 cgctgagcca tttttcaatg ttagcggtat tagtgtttgg tttttactgg ctgattcgcc
    28261 tgtgcgcact gccgctatat cgcaaaatgg gtcagtgggc gaggcaaaaa aatcgtgagc
    28321 gcagcaactg gttgcagctt ccggcgatga ttattggggc gtttattatc gacctgctgt
    28381 tactggccct gacattattt gtcggccagg tattaagcga caacctgaat gcaggcagtc
    28441 gcaccatcgc tttccaacaa agtttgtttc tcaacgcctt tgccctgatt gaatttttca
    28501 aagccgtact acgcctgatt ttttgcccaa acgtggcgga gctgcgcccg ttcacgattc
    28561 aggacgagag cgcccgttac tggggtcgtc gcctgagctg gttaagcagc ctgattggct
    28621 atggcctgat tgtggccgtg ccgattatct ctaatcaggt gaatgtacag ataggtgcgc
    28681 tggcgaacgt catcattatg ctgtgcatga ccgtctgggc gttgtacctg atcttccgaa
    28741 ataaaaaaga gattacccag catatgctca actttgcgga gcattcgctg gcttttttca
    28801 gcctgtttat ccgcgccttt gcgctggtgt ggcactggct ggcaagcgcc tattttatca
    28861 tgctgttttt cttttcgttg ttcgatccgg gcaacagtct gaaatttatg atgggtgcaa
    28921 cggtgcgcag cctggcgatt attggtatcg cagcgtttgt ttccggtatg ttttcccgct
    28981 ggctggcgaa aaccatcacc ctctcgccac atactcagcg taactatccg gagctacaaa
    29041 aacggctgaa tggctggctt tcggcggcgc tgaaaacggc gcgtattctg accgtctgcg
    29101 tggcagtaat gttgctgttg agcgcatggg gattgttcga tttctggaac tggctgcaaa
    29161 acggcgcggg gcagaaaacc gtagatatcc tgatccgcat cgcactcatt cttttcttct
    29221 cggcggttgg ctggacggtg ctcgccagtt tgatcgaaaa ccggctggct tcggatattc
    29281 atggccgccc gctacccagc gcccgtacgc gtaccctgct gacgctgttc cgtaacgcgc
    29341 tggcggtgat tatcagtacc atcaccatca tgattgtgtt gtcggaaatc ggcgtcaata
    29401 tcgcgccatt gctggcaggt gccggggcat taggtctggc tatctcgttt ggttcgcaaa
    29461 cgctggtaaa agatattatc accggggtat ttattcagtt tgaaaacggc atgaacactg
    29521 gagatttggt gactatcggg ccgttgaccg gcacagtgga acggatgtcg attcgctccg
    29581 tgggcgtgcg ccaggatacc ggggcgtatc acatcattcc gtggtcttcg ataaccacct
    29641 ttgctaactt cgtccgcggt attggttcgg tagtggcaaa ctacgatgtt gatcgccatg
    29701 aagatgctga taaagccaat caggcactga aagatgcggt aacggaattg atggaaaacg
    29761 aagaaattcg cgggctgatt attggtgaac cgaactttgc cgggattgtc ggcttaagca
    29821 ataccgcgtt tacactgcgt gtttcgttca ccacgctgcc actcaaacag tggacggtac
    29881 gctttgccct cgacagccag gtgaaaaaac atttcgacct ggcgggcgtt cgcgcgccag
    29941 tgcagactta tcaggtgctg cctgctccgg gcgcgacccc ggctgaaccg ctgccgccgg
    30001 gggagccaac gctttaacgt tggcgattaa caaaacggcg gcgctgctcg tcgttcataa
    30061 aggtccaggc aataaagcga ctctgctttt gcccctgggc catctctttt tttaccacct
    30121 tcaccgcgcc cacgtcggtc agagcacgat acaacggcgg taagttttca ccacgagata
    30181 ccagcgaggt aaaccacatc acctgcttcg cgaagccttt gctctcttca atcatctttt
    30241 tgataaagtt gacttcaccg ccttcacacc acaactcctg ttgctgacca ccaaagttca
    30301 gtgcatcgtc tttgttcagc cccaggttac ggcgtttacg ctcactacct gcccgcgccg
    30361 cagcggcgga atcgtggaat ggcgggttac acaaggtcgc gtcgtattgc tcgtttttgt
    30421 ggatgatgcc gttaaaaatc gccccactct ctttttgccg acgcagacga atggcgcggt
    30481 taagccccgg attagcactg ataatcgcct gcgcactgct taacgcctgg ctgctggttt
    30541 cactaccggt aaaacgccag ccatattcat gtacgccaat cagcggataa atacagttcg
    30601 caccaacgcc gatatccaga atgctggcat tcgctggaat tgttccgctt gcttcagcca
    30661 gtaaatcggc aaggtgatga atataatccg cccggcccgg tactggtggg cagagaaaac
    30721 cgtcggggat atcccagttc gctacggcgt aaaaatgggc cagcaacgcc ttattgagcg
    30781 ccttcaccgc cagcggattg gcaaagtcta cgctttgctc ccctgcgggt gtaagcgtga
    30841 ggaattgcct gagttcagga ttgacctgac aaagcgtggc gagatcgtag cggctgtgat
    30901 gacggttgcg cggatgcaac cccggtttct gggcggacat agcattctcc tttaagcagc
    30961 ggcgtaagat acccgcataa cgcgacaggg taaatatctg gctgcgttta tcattcccca
    31021 aaaccttaac atggctatgg tttatggcaa ttcatcataa aaatcagcat tatgcatgtc
    31081 attgatttgt cttattttgt aaaaatatat cgttcaaaaa ataagcattt ctctgtcaat
    31141 tttttgcgtc atttgcatcg cgcaggcgac ctacacttat caggcactac ccgttgtatt
    31201 tatgaggatg tcattatgaa aaagtgcctc acactactga ttgccaccgt cctgagcgga
    31261 atctctctca cggcttatgc cgcgcaaccg atgagtaacc tggacagcgg tcagctacgg
    31321 cccgccggta ccgtttcggc aactggcgca tcaaacctaa gcgatctgga ggataaactg
    31381 gcagaaaaag cgcgcgaaca aggcgcgaaa ggttatgtca ttaattccgc aggcggaaat
    31441 gaccagatgt taggtactgc aaccatctac aaataacccc accaacgtaa ctgcattcgc
    31501 ctctggatgc agttttcgcc tgttttgtaa tcaaaaaaca tcaaatatga acgcaatgta
    31561 aataaatgta tttctttttc gcgcaatggg tgatagaaaa tcgcatcaag tgataatgct
    31621 tatcaaaatt attatcactt gcacgaacac tatcacggga ttaacagtgg catcgcatcc
    31681 gcagagatgc tttctcgtgg cagtgaaaat ttcaacatat aagaaaaagt cacctgcaaa
    31741 atggaaaaca atcgcaattt ccctgccaga caatttcatt cgctcacgtt ctttgccggt
    31801 ctttgtattg gtattacgcc tgtggcacag gcactcgccg ccgaagggca agctaacgcg
    31861 gatgacacgc tggttgtcga agcatcaacg ccttcgcttt atgcgccaca acaatctgcc
    31921 gatccgaaat tctcgcgtcc ggtagcggat actacccgca cgatgacggt gatttctgaa
    31981 caagtgatta aagatcaggg cgcaaccaac cttaccgacg cgctcaaaaa cgtccccggc
    32041 g
//
LOCUS       DAZPCS010000029        31954 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZPCS010000029 DAZPCS010000000
VERSION     DAZPCS010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31954
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.029"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..866)
                     /gene="rarD"
                     /locus_tag="ACHUQ7_001367"
     CDS             complement(<1..866)
                     /gene="rarD"
                     /locus_tag="ACHUQ7_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFR1134849.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAI"
     gene            complement(918..1385)
                     /gene="yigI"
                     /locus_tag="ACHUQ7_001368"
     CDS             complement(918..1385)
                     /gene="yigI"
                     /locus_tag="ACHUQ7_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFR1134850.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1550..2419
                     /gene="pldA"
                     /locus_tag="ACHUQ7_001369"
     CDS             1550..2419
                     /gene="pldA"
                     /locus_tag="ACHUQ7_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFR1134851.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2552..4381
                     /gene="recQ"
                     /locus_tag="ACHUQ7_001370"
     CDS             2552..4381
                     /gene="recQ"
                     /locus_tag="ACHUQ7_001370"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFR1134852.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSRGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4445..5065
                     /gene="rhtC"
                     /locus_tag="ACHUQ7_001371"
     CDS             4445..5065
                     /gene="rhtC"
                     /locus_tag="ACHUQ7_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFR1134853.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5127..5747)
                     /gene="rhtB"
                     /locus_tag="ACHUQ7_001372"
     CDS             complement(5127..5747)
                     /gene="rhtB"
                     /locus_tag="ACHUQ7_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFR1134854.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            5858..6880
                     /gene="pldB"
                     /locus_tag="ACHUQ7_001373"
     CDS             5858..6880
                     /gene="pldB"
                     /locus_tag="ACHUQ7_001373"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFR1134855.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATQTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            6888..7688
                     /gene="yigL"
                     /locus_tag="ACHUQ7_001374"
     CDS             6888..7688
                     /gene="yigL"
                     /locus_tag="ACHUQ7_001374"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFR1134856.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGISKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            7764..8663
                     /gene="bioP"
                     /locus_tag="ACHUQ7_001375"
     CDS             7764..8663
                     /gene="bioP"
                     /locus_tag="ACHUQ7_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFR1134857.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRSNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQYNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8551..9504)
                     /gene="metR"
                     /locus_tag="ACHUQ7_001376"
     CDS             complement(8551..9504)
                     /gene="metR"
                     /locus_tag="ACHUQ7_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFR1134858.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALGNFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            9740..12001
                     /gene="metE"
                     /locus_tag="ACHUQ7_001377"
     CDS             9740..12001
                     /gene="metE"
                     /locus_tag="ACHUQ7_001377"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFR1134859.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALSHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDNVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(12040..12855)
                     /locus_tag="ACHUQ7_001378"
     CDS             complement(12040..12855)
                     /locus_tag="ACHUQ7_001378"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFR1134860.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            13117..13878
                     /gene="udp"
                     /locus_tag="ACHUQ7_001379"
     CDS             13117..13878
                     /gene="udp"
                     /locus_tag="ACHUQ7_001379"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFR1134861.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTVSSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            14019..15446
                     /gene="rmuC"
                     /locus_tag="ACHUQ7_001380"
     CDS             14019..15446
                     /gene="rmuC"
                     /locus_tag="ACHUQ7_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFR1134862.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            15541..16296
                     /gene="ubiE"
                     /locus_tag="ACHUQ7_001381"
     CDS             15541..16296
                     /gene="ubiE"
                     /locus_tag="ACHUQ7_001381"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFR1134863.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            16310..16915
                     /gene="ubiJ"
                     /locus_tag="ACHUQ7_001382"
     CDS             16310..16915
                     /gene="ubiJ"
                     /locus_tag="ACHUQ7_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFR1134864.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            16912..18552
                     /gene="ubiB"
                     /locus_tag="ACHUQ7_001383"
     CDS             16912..18552
                     /gene="ubiB"
                     /locus_tag="ACHUQ7_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFR1134865.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            18631..18900
                     /gene="tatA"
                     /locus_tag="ACHUQ7_001384"
     CDS             18631..18900
                     /gene="tatA"
                     /locus_tag="ACHUQ7_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFR1134866.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            18904..19419
                     /gene="tatB"
                     /locus_tag="ACHUQ7_001385"
     CDS             18904..19419
                     /gene="tatB"
                     /locus_tag="ACHUQ7_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFR1134867.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            19422..20198
                     /gene="tatC"
                     /locus_tag="ACHUQ7_001386"
     CDS             19422..20198
                     /gene="tatC"
                     /locus_tag="ACHUQ7_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFR1134868.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSSEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            20240..21022
                     /gene="tatD"
                     /locus_tag="ACHUQ7_001387"
     CDS             20240..21022
                     /gene="tatD"
                     /locus_tag="ACHUQ7_001387"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFR1134869.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(21019..21507)
                     /gene="rfaH"
                     /locus_tag="ACHUQ7_001388"
     CDS             complement(21019..21507)
                     /gene="rfaH"
                     /locus_tag="ACHUQ7_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFR1134870.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            21674..23167
                     /gene="ubiD"
                     /locus_tag="ACHUQ7_001389"
     CDS             21674..23167
                     /gene="ubiD"
                     /locus_tag="ACHUQ7_001389"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFR1134871.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDNFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            23213..23914
                     /gene="fre"
                     /locus_tag="ACHUQ7_001390"
     CDS             23213..23914
                     /gene="fre"
                     /locus_tag="ACHUQ7_001390"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFR1134872.1"
                     /translation="MTTLSCKVTSVAAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDLPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHL
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(24106..25269)
                     /gene="fadA"
                     /locus_tag="ACHUQ7_001391"
     CDS             complement(24106..25269)
                     /gene="fadA"
                     /locus_tag="ACHUQ7_001391"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFR1134873.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDTQACLVGGVEHIGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(25279..27468)
                     /gene="fadB"
                     /locus_tag="ACHUQ7_001392"
     CDS             complement(25279..27468)
                     /gene="fadB"
                     /locus_tag="ACHUQ7_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFR1134874.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     QEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            27658..28989
                     /gene="pepQ"
                     /locus_tag="ACHUQ7_001393"
     CDS             27658..28989
                     /gene="pepQ"
                     /locus_tag="ACHUQ7_001393"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFR1134875.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRYTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            28989..29603
                     /locus_tag="ACHUQ7_001394"
     CDS             28989..29603
                     /locus_tag="ACHUQ7_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFR1134876.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            29642..31093
                     /gene="trkH"
                     /locus_tag="ACHUQ7_001395"
     CDS             29642..31093
                     /gene="trkH"
                     /locus_tag="ACHUQ7_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFR1134877.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            31105..31650
                     /gene="hemG"
                     /locus_tag="ACHUQ7_001396"
     CDS             31105..31650
                     /gene="hemG"
                     /locus_tag="ACHUQ7_001396"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFR1134878.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         7430 a         8137 c         8743 g         7644 t
ORIGIN      
        1 taaatcgcgt ccatcacaaa aattgccagc gccacccaaa taaaggtgaa agtcaccatc
       61 ttatcggcac ccggtttttc accataaaac gtcacagcca gcaggaacat cagcgtcggg
      121 ccaatgtact gaaaaaagcc taacgttgag agacgcaggc gcgtggcagc ggcagtaaaa
      181 cacaacagcg gtacggtagt gacaataccg gcggcgatca gcagtaaatt cagcgacatc
      241 gggttttgcc ccatatggct ggttgaactg tcggcaatag caaacaggta aatcgccgcc
      301 acgggcagca gccacatggt ttcaattaac atgccggttt gtgcttctac cgcaatcttc
      361 ttgcgcacca ggccgtagaa ggcaaaacta aatgccagcc ccagcgcgat aataggtagc
      421 gaaccaaaag tccacagctg gaccaacacg ccacatatcg ccagaatcac cgccagccat
      481 tgcatccggc ggaatcgctc gccgaggaaa atcatcccca gcacaatgtt caccagcggg
      541 ttaataaagt aaccaaggct cgcttccagc atatggtgat tgttcaccgc ccagataaac
      601 agtagccagt tgccaccaat cagtacggca gagactgcca gcataaaaat tttctgtggc
      661 gtctgaatca gcgtttttaa ataggaccac tggcggcaaa tgctcatcag caccaccata
      721 aagaaaaacg accagatcac gcgatgcgtc aggatctcat cggcgggcac gtagtaaatc
      781 agcttgaagt atgctggcgc aataccccaa ataaaataag cggcaagagc gagtaatacg
      841 ccctgccgcg tttgttttgc atccatcggg aatactcatt tttaattggt aacagcagtt
      901 tacctgcttt tatgtcttca acctaccata taggtggcgg tggcactggc aatataaagc
      961 tgttcttcat tgtgtaattc aacgcgggcg acggcgactt tattgcctgc acgcaacagg
     1021 ctactggtag cagtaaaacg ctcgcccctg cctgggcgca gataatcaac gcgaagatca
     1081 atggtcccca tccgcgatag ccgctggcgt agttcatctt cactgatggt ttcgtggcgg
     1141 gttaaggtac ttcccacgca caccagaccg gcggcgacat ccagcgccga cgcaatgact
     1201 ccgccgtgca aaatgctttg cgcccagtta cccaccatca tcggctgatt tttaaaggcc
     1261 agctgtgcga actctttttc gtaacgctcc agttccatcc ccaatgcgcg gttaaatggc
     1321 atgtgataaa caaacatctc acccactaat ttcagggctt gttcagcggt cagtacggca
     1381 gacatatcat ccttacactt cattggttaa tgaaatgttg attttatgct tctttgttgt
     1441 tggtttctac tttaggaagg gataactaac ggcaatggag ttaagtatgt aaaatagccc
     1501 gcagaaaaac attcacctta tcaataattc gttacggaga acacgaccga tgcggactct
     1561 gcagggctgg ttgttgccgg tgtttatgtt gcctatggca gtatatgcac aagaggcaac
     1621 ggtgaaagag gtgcatgacg cgccagcggt gcgtggcagt attatcgcca atatgctgca
     1681 ggagcatgac aatccgttca cgctctatcc ttatgacacc aactacctca tctacaccca
     1741 aaccagcgat ctgaataaag aagcgattgc cagttacgac tgggcggaaa atgcgcgtaa
     1801 ggatgaagta aagtttcagt tgagcctggc atttccgctg tggcgtggga ttttaggccc
     1861 aaactcggtg ttgggtgcgt cttatacgca aaaatcctgg tggcaactgt ccaatagcga
     1921 agagtcttca ccgtttcgtg aaaccaacta cgaaccgcaa ttgttcctcg gttttgccac
     1981 cgattaccgt tttgcaggtt ggacgctgcg cgatgtggag atggggtata accacgactc
     2041 taacgggcgt tcagacccga cctcccgcag ctggaaccgc ctttatactc gcctgatggc
     2101 agaaaacggt aactggctgg tagaagtgaa gccgtggtat gtggtgggta atactgacga
     2161 taacccggat atcaccaaat atatgggtta ctaccagctt aaaatcggct atcacctcgg
     2221 tgatgcggtg ctcagtgcga aaggacagta caactggaac accggctacg gcggcgcgga
     2281 gttaggctta agttacccga tcaccaaaca tgtgcgcctt tatactcagg tttacagcgg
     2341 ctatggcgaa tcgctcatcg actataactt caaccagacc cgtgtcggtg taggggttat
     2401 gctaaacgac ttgttttgat gaacggttga gtggttggca aatctggaat ccagcatcca
     2461 ggattaccct ctcagagact aaaagcattg cagtttctcg cgcaggcgct gaaaatagcg
     2521 cctgttttta tttcaggcaa tcggggtgaa tgtggcgcag gcggaagtgt tgaatctgga
     2581 gtccggagct aaacaggttt tacaagaaac ctttggctac caacagtttc gccccggcca
     2641 ggaagaaatt atcgacactg tgctttccgg tcgcgattgc ctggttgtta tgcccactgg
     2701 tggcggaaaa tccctttgct atcaaattcc tgccttattg ctaaacggcc ttaccgtggt
     2761 tgtttcaccg ctgatttcgt tgatgaaaga tcaggtggat caactacagg ccaacggcgt
     2821 ggcggcggcg tgccttaact cgacgcaaac ccgcgaacag caacttgaag tgatgacagg
     2881 ctgccgcacc gggcaaattc gcttactgta tatcgccccg gaacgcctga tgctggataa
     2941 ctttcttgag catctggcgc actggaatcc ggtgttatta gccgtcgatg aagcgcactg
     3001 tatctcccaa tggggtcacg atttccgccc ggaatatgcc gcgctcggtc agttgcgcca
     3061 gcggttcccg acgctgccgt ttatggcgct gaccgccaca gccgacgaca ccacgcgcca
     3121 ggatatcgtg cgcctgctgg ggctgaacga tccgctgatt caaatcagca gttttgaccg
     3181 tccgaatatt cgctacatgc tgatggagaa attcaaaccg ctcgatcagt tgatgcgcta
     3241 cgtgcaggaa cagcgcggta agtctggcat tatctactgc aacagccggg cgaaagtaga
     3301 agacaccgct gcgcgcctgc aaagcagggg tattagcgcg gcggcctatc atgccgggct
     3361 ggaaaataat gtccgcgccg acgtgcagga gaaattccag cgcgatgatc tgcaaattgt
     3421 ggtggcgacg gtggcgttcg gcatgggcat caataaacca aacgttcgct tcgtggtcca
     3481 ctttgatatt ccgcgcaata tcgaatccta ttatcaggaa accggtcgcg ccgggcgtga
     3541 tggcctgcct gcggaagcga tgctgtttta cgatccggct gatatggcgt ggctgcgccg
     3601 ttgtctggaa gagaagccgc aggggcagtt gcaggatatc gagcgccaca aactcaatgc
     3661 gatgggcgcg tttgccgaag cgcaaacttg ccgtcgtctg gtattgctga actattttgg
     3721 cgaagggcgt caggagccgt gcgggaactg cgatatctgc ctcgatccgc cgaaacagta
     3781 cgacggttca accgatgctc agattgccct ttccaccatt ggtcgtgtga atcagcggtt
     3841 tgggatgggt tatgtggtgg aagtgattcg tggtgctaat aaccagcgta tccgcgacta
     3901 tggtcatgac aaactgaaag tctatggtat gggccgtgat aaaagccatg aacattgggt
     3961 gagcgtgatc cgccagctga ttcatctcgg cctggtgacg caaaatattg cccagcattc
     4021 tgccctacaa ctgacagagg ccgcgcgccc ggtgctgcgc ggcgaatcct ctttgcaact
     4081 tgccgtgccg cgtatcgtgg cgctcaaacc gaaagcgatg cagaaatcgt tcggcggcaa
     4141 ctatgatcgc aaactgttcg ccaaattacg caaactgcgt aaatcgattg ccgatgaaag
     4201 taatgtcccg ccgtacgtgg tgtttaacga cgcaaccttg attgagatgg ctgaacagat
     4261 gccgatcacc gccagcgaaa tgctcagcgt taacggcgtt gggatgcgca agctggaacg
     4321 ctttggtaaa ccgtttatgg cgctgattcg tgcgcatgtc gatggcgacg acgaagagta
     4381 gtcagcagca taaaaaagtg ccagtatgaa gactccgtaa acgtttcccc cgcgagtcaa
     4441 atgtatgttg atgttatttc tcaccgtcgc catggtgcac attgtggcgc ttatgagccc
     4501 cggtcccgat ttcttttttg tctctcagac cgctgtcagt cgttcccgta aagaagcgat
     4561 gatgggcgtg ctgggcatta cctgcggcgt aatggtttgg gctgggattg cgttgcttgg
     4621 cctgcatttg attatcgaaa aaatggcctg gctgcatacg ctgattatgg taggcggtgg
     4681 cctgtatctc tgctggatgg gttaccagat gctacgcggt gcactgaaaa aagaggctgt
     4741 atctgcgcct gcaccacagg ttgagctggc gaaaagcggg cgcagtttcc tgaaaggctt
     4801 actgaccaat ctcgccaatc cgaaagcgat tatctatttt ggttcggtat tctcattgtt
     4861 tgtcggcgat aacgttggca ctaccgagcg ctggggcatt ttcgcgttga ttattgtcga
     4921 aacactggcg tggtttaccg tcgttgccag tctgtttgcc ctgccgcaaa tgcgccgtgg
     4981 ttatcaacgt ctggcgaagt ggattgatgg ttttgccggg gcgttatttg ccggatttgg
     5041 cattcatttg attatttcgc ggtgatgcca gacgcgtctt cagagtaagt cggataaggc
     5101 gtttacgccg catccgacat tatttttcac gcatgcctcg ccgatgctaa cagcgctccc
     5161 accagcataa acaacgagcc gaaaatctta ttcagcgcct tcatctgctt tggtccttta
     5221 atccatagag caatcctttg agcaagggtg gcgtaaccga tcatcacaat aatatcgacc
     5281 acaatagtgg tgacgccgag cacgatatac tgcatcagtt gcggctgttg cggcatgatg
     5341 aattgcggaa atagcgccgc cagaaacaca atacttttgg gattggtgag attcacaaaa
     5401 actgcgcgct ggaacaaatg tcgacgcgat tgagtagagg ccagcgattt aagatcaatt
     5461 gcgccagcgg cgcgccactg ctggattccc agccaaatca agtaagccgc gcctgcccac
     5521 tttaacactt caaacgcaat cactgagcgg gaaaatagcg tccccaaccc cacgccaacc
     5581 agcacaatat gaatcgccag tccggtctga agcccagcaa tagacgccac cgcgccgcga
     5641 taaccgtggt tgagcgaggt ggtcatagtg ttgattgcac cagagcctgg cgacaggctt
     5701 aaaatgatcg atgtcagcag gtaggcaaac caccattcta aggtcatgat gaactcccgg
     5761 tgtgtctatt tttgtgccac aatacgctac tgtcgcagcg ttgtgtcagg cacgcttaaa
     5821 aaaacgattt tacgtggttt aagaggcaga ttacccgatg tttcagcagc aaaaagactg
     5881 ggaaacaaga gaaaacgcgt ttgctgcttt taccatggga ccgctgactg atttctggcg
     5941 tcagcgtgat gaagcagagt ttactggtgt ggatgacatt ccggtgcgct ttgtccgttt
     6001 tcgcgcacag caccatgacc gggtggtagt catctgcccg gggcgtattg agagctacgt
     6061 aaaatatgcg gaactggcct acgacctgtt ccatttgggg tttgatgtct taatcatcga
     6121 ccatcgcggg cagggacgtt ccggtcgcct gttagccgat ccgcatctcg ggcatgttaa
     6181 tcgctttaat gattatgttg atgatctggc ggcattctgg cagcaggagg ttcagcccgg
     6241 tccgtggcgt aaacgctata tactggcaca ttcgatgggc ggtgcgatct ccacattatt
     6301 tctgcaacgc catccaggtg tatgtgacgc cattgcgcta actgcgccaa tgtttgggat
     6361 cgtgattcgt atgccgtcat ttatggcacg gcagatcctc aactgggccg aagcgcatcc
     6421 acgtttccgt gatggctatg caataggcac cgggcgctgg cgcgcgttgc cgtttgctat
     6481 caacgtactg acccacagca gacagcgata tcgacgtaac ttacgcttct atgctgatga
     6541 cccaacgatt cgcgtcggtg ggccgaccta ccattgggta cgcgaaagta ttctggctgg
     6601 cgaacaggtg ttagccggtg cgggtgatga cgccacgcaa acgcttctct tacaagctga
     6661 agaggaacgc gtggtggata accgcatgca cgaccgtttt tgtgaactcc gcaccgccgc
     6721 gggccatcct gtcgaaggag gacggccgtt ggtaataaaa ggtgcttacc atgagatcct
     6781 ttttgaaaag gacgcaatgc gctcagtcgc gctccacgcc atcgttgatt ttttcaacag
     6841 gcataactca cccagcggaa accgctctac agaggtttaa atttcttatg taccaggttg
     6901 ttgcgtctga tttagatggc acgttacttt ctcccgacca tacgttatcc ccttacgcca
     6961 aagaaactct gaagctgctc accgcgcgcg gcatcaactt tgtgtttgcg accggtcgtc
     7021 accacgttga tgtggggcaa attcgcgata atctggagat taagtcttac atgattacct
     7081 ccaatggtgc gcgcgttcac gatctggatg gcaatctgat ttttgctcat aacctggatc
     7141 gcgacattgc cagcgatctg tttggcgtag tcaacgacaa tccggacatc attactaacg
     7201 tttatcgcga cgacgaatgg tttatgaatc gccatcgccc ggaagagatg cgctttttta
     7261 aagaagcggt gttcaaatat gcgctgtatg agccgggatt actggagccg gaaggcatca
     7321 gcaaagtgtt tttcacctgc gattcccatg aacaactgct gccgctggag caggcgatta
     7381 acgctcgctg gggcgatcgc gtcaacgtca gtttctctac cttaacctgt ctggaagtga
     7441 tggcgggcgg cgtgtcgaaa ggccatgcgc tggaagcagt ggcgaagaaa ctgggctaca
     7501 gcctgaagga ttgtattgcg tttggtgacg ggatgaacga cgccgaaatg ctgtcgatgg
     7561 cggggaaagg ctgcattatg ggcagtgcgc accagcgtct gaaagatctg catccggagc
     7621 tggaagttat tggtactaat gccgacgacg cggtgccgca ttatctgcgt aaactctatt
     7681 tatcgtaatc gttctttatt tggtcagttg tcaacctgat acttcgctac aatggatact
     7741 cgttaatcaa agagttttcc attgtggcgt tacttatcat caccacgatt ctgtgggcct
     7801 tctcctttag cttttatggc gagtaccttg cggggcacgt cgatagctat tttgcggtgc
     7861 tggtgcgcgt tggcttggcg gcactcgttt ttctgccgtt tctgcgtacc cgtagcaata
     7921 gcctgaaaac ggtcggcctg tatatgctgg tgggcgcgat gcagctcggc gtgatgtata
     7981 tgctgagttt ccgcgcttat ctctacctga cggtctccga attgttgctg ttcaccgtac
     8041 tgacgccgct ctacatcacg ctgatttatg acatcatgag taagcgccgt ctgcgctggg
     8101 gctatgcttt tagcgccttg ctggcggtga ttggtgccgg gattattcgc tatgatcagg
     8161 tcaccgacca tttctggact ggcttgttgc tggtgcaact ctccaatatc acttttgcca
     8221 tcggcatggt gggttacaaa cgcctgatgg aaactcgccc gatgccgcag tataacgcct
     8281 ttgcgtggtt ctatcttggc gcgttcctgg tggcggtaat tgcatggttc ttgctgggaa
     8341 atgcgcagaa aatgccgcaa accacgttgc aatggggcat tctggtgttt cttggcgtgg
     8401 tggcttccgg gattggctac tttatgtgga actacggcgc gacgcaggtg gacgccggaa
     8461 cgctgggcat tatgaataat atgcacgttc cggcagggct gctggtaaac ctggctatct
     8521 ggcaccaaca gccgcactgg ccaacgttta ttacaggcgc gctggtgatc ctggcctcac
     8581 tgtgggtgca tcgtaagtgg gtcgctccgc gctcttcaca aacggcagat gatcgcaggc
     8641 gtgattgcgc gctgagcgaa taaacgcttc cgtaactggc tgacgctgct cgccatcgcg
     8701 cacggcggcg tacagtcggc tccacaagcc ttcgcccagg gttttggtca ccaccagacc
     8761 ctggcgctca aaactctcta ctacccaatg cggtagcgcg gcaataccca tccgcgcggc
     8821 aaccatttga atcaacaata aggtgttatc gacgcttttc agtgacgggc tgacgcctgc
     8881 cggctgaaga aaatgccgcc agacatccag tcgactacgc tgcaccggat aaattaatag
     8941 cgtctcgctg gcgagatctt ccggtgtaat acgcgttttt gccgccagtg gatggtcagg
     9001 tgctaacacc aggcgtactt cataatcgaa catcggcgaa taatgcaggc cactgcgcgg
     9061 caggatatcg gaggtcatca ccagatccag ctcgccctgt tgcaacgcgg gctgcgggtc
     9121 aaacgtcacg cccgatttaa aatccatctc tacctgcggc cagttcttat ggaaatttcc
     9181 taaagcgggt gtcagccact gaatacagct atggcactca atggcaatgc gcagacgcgt
     9241 ctgctgcggt tcattgcagg cttgcagtgc ctggctgatt tgcggcagca cctggtttgc
     9301 cagttgcaac aggatttctc cctgcggtgt aaagcgtagc ggctggctct tacgcacaaa
     9361 tagccggaag ccaaggcgtt gttccagatc gctaaactgg tgagacaggg cggattgcgt
     9421 ctgatgcaac gttgccgcag cggctgcgag cgagccgcag ttccgcaacg cttgtagcgt
     9481 tttcaggtgt tttacttcga tcatgaaagt ccttcacttc ggcatgaata atttgcgctt
     9541 gaggaatata cagtaaccgc caattatgga tgtgtaaaca tctggacggc taaaatcatt
     9601 cgtcttttaa atttatggtg cgttggctgc gtttctccac cccggtcact tacttcagta
     9661 agctcccggc gatgaataaa cttgccgcct tccctaaatt caaaatccat aggatttaca
     9721 tataattaga ggaagaaaaa tgacaatatt gaatcacacc ctcggtttcc ctcgcgttgg
     9781 cctgcgtcgc gagctgaaaa aagcgcaaga aagttattgg gcggggaact ccacgcgtga
     9841 agaactgctg acagtagggc gtgaactgcg tgctcgtcac tgggatcaac aaaagcaagc
     9901 gggtatcgac ctgctgccgg tgggcgattt tgcctggtac gatcatgtac tgaccaccag
     9961 tttgctgctg ggtaatgttc cgccacgtca tcagaacaaa gacggttcgg tagatatcga
    10021 caccctgttc cgtattggtc gtggacgtgc gccgactggc gaacctgcgg cggcagcgga
    10081 aatgaccaaa tggtttaaca ccaactatca ctacatggtg ccggagttcg ttaaaggcca
    10141 acagttcaaa ctgacttgga cgcagctgct ggaggaagtg gacgaggcgc tggcgctgag
    10201 ccacaacgtt aagcctgtgc tgctggggcc ggttacttac ctgtggctgg ggaaagtgaa
    10261 aggtgaacag tttgaccgcc ttagcctgct gaacgacatt ctgccggttt atcagcaagt
    10321 gctggcagaa ctggcgaaac gcggcatcga gtgggtacag attgatgaac ccgcgctggt
    10381 actggaacta ccacaggcgt ggctggacgc atacaaaccc gcttacgacg cgctccaggg
    10441 acaggtgaaa ctgctgctga ccacctattt tgaaggcgta acgccaaatc tcgacacgat
    10501 tactgcgctg cctgttcagg ggctgcatgt ggaccttgta catggtaaag ataacgttgt
    10561 tgaactgcac aagcgcctgc cttctgactg gctgctgtct gcgggtctga tcaatggtcg
    10621 taacgtctgg cgcgccgatc ttactgagaa atatgcgcaa attaaggaca ttgtcggcaa
    10681 acgtgatttg tgggtggcat cttcctgctc gttgctgcac agccccatcg acctgagcgt
    10741 ggaaacgcgt cttgatgcag aagtgaaaag ctggtttgcc ttcgccctac aaaaatgcca
    10801 tgaactggca ctgctgcgcg atgcgctgaa cagtggtgac acggcagctc tggcagagtg
    10861 gagcgcccca attcaggcgc gtcgtcactc tactcgtgta cataatccgg cggtagaaaa
    10921 gcgtctggcg gcgatcaccg cccaggacag ccagcgtgcg aatgtctatg aagtgcgtgc
    10981 tgaagcccag cgtgcgcgtt ttaaactgcc agcgtggccg accaccacga ttggttcctt
    11041 cccgcaaacc acggaaattc gtaccctgcg tctggatttc aaaaagggca atctcgacgc
    11101 caacaactac cgcacgggca ttgcggaaca tatcaagcag gccattgttg agcaggaacg
    11161 tttgggactg gatgtgctgg tacatggcga ggccgagcgt aatgacatgg tggaatactt
    11221 tggcgagcac ctcgacggat ttgtctttac gcaaaacggt tgggtacaga gctacggttc
    11281 ccgctgcgtg aagccaccga ttgtcattgg tgacattagc cgcccggcac cgattaccgt
    11341 ggagtgggcg aagtatgcgc aatcgctgac cgacaaaccg gtgaaaggga tgctgacggg
    11401 gccggtgacc atactctgct ggtcgttccc gcgtgaagat gtcagccgtg aaaccatcgc
    11461 caaacagatt gcgctggcgc tgcgtgatga agtggccgat ctggaagccg ctggaattgg
    11521 catcatccag attgacgaac cggcgctgcg cgaaggttta ccgctgcgtc gtagcgactg
    11581 ggatgcgtat ctccagtggg gcgtagaggc cttccgtatc aacgccgccg tggcgaaaga
    11641 tgacacacaa atccacactc acatgtgtta ttgcgagttc aacgacatca tggattcgat
    11701 tgcggcgctg gacgcagacg tcatcaccat cgaaacctcg cgttccgaca tggagttgct
    11761 ggagtcgttt gaagagtttg attatccaaa tgaaatcggt cctggcgtct atgacattca
    11821 ctcgccaaac gtaccgagcg tggaatggat tgaagccttg ctgaagaaag cggcaaaacg
    11881 cattccggca gagcgcctgt gggtcaaccc ggactgtggc ctgaaaacgc gcggctggcc
    11941 agaaacccgc gcggcactgg cgaacatggt gcaggcggcg cagaatttgc gtcgggggta
    12001 aatccaaacc gggtggtaat accacccggt cttttctcat tacagcgact tcttcccacc
    12061 atactgctta aaccattcca gcatacgctg ccagccatct ttcgcagatt cggcatgata
    12121 gctcgggcga taatcagcgt tgaatgcatg cccggcatcc gggtacacga taatctctgc
    12181 tttcgcatta gcagcccgca gcgcctggcg catcgtttca acgctctcct gcggaatgct
    12241 gttatcctga ccaccatata agccgagaac cggcgcgtta agatcggttg cgatatcaac
    12301 aggttgtttc ggtgaattca gcgacttgtc gcccgtcagt ttgccgtacc acgccactgc
    12361 ggcttttagc tgtggattat gcgcggcata cagccaggtg atacgtccac cccagcagaa
    12421 tccggtgatc attaaacgat gaacatcgcc gccgttgcgg gaagcccaac tggcgacatg
    12481 atcgagatcg gccagcacct gcgagtcagg cacttttgct accagaccgc taagcaacgt
    12541 ggggatatcg gcaaaatcat tcggatcgcc ttcgcggaag taaagttcag gtgcgatagc
    12601 cagatacccc tccagcgcca gacggcgaca aatgtcgcgg atatgttcat gcacgccaaa
    12661 aatttcctgc actacaatga ccactggcag tgggccatcg ctttgctttg gtctggcatg
    12721 gtaagcaggc atgttatccc cttgtgaagg gatagaggtg aagcccgcca caatcgcgtc
    12781 gtccggggtc tgaacgatgg tcgaagcgag aggcgatgca gcaggtgcaa atccagattg
    12841 ttgtgttgtt gccatggtat tctccgtacc cttataaaaa tgttgcgcaa tgttaactat
    12901 agtcagcatg caacaaatca cattgcctga atcggctcat cttttatgca gtcctgcaga
    12961 atgaagagtg atttatgtga tatgaatcac ttttggtggg taaatttatg caacgcattt
    13021 gcgtcatggt gatgagtatc acgaaaaaat gttaaaccct tcggtaaagt gtctttttgc
    13081 ttcttctgac taaaccgatt cacagaggag ttgtatatgt ccaagtctga tgtttttcat
    13141 ctcggcctca ctaaaaacga tttacaaggg gctacgcttg ccatcgtccc tggcgacccg
    13201 gatcgtgtgg aaaagatcgc cgcgctgatg gataagccgg ttaagctggc atctcaccgc
    13261 gaattcacta cctggcgtgc agagctggat ggtaaacctg ttatcgtctg ctctaccggt
    13321 atcggcggcc cgtctacctc tattgctgtt gaagagctgg cacagctggg cattcgcacc
    13381 ttcctgcgta tcggtacaac gggcgctatt cagccgcata ttaatgtggg tgatgtcctg
    13441 gttaccacgg cgtctgtccg tctggatggc gcgagcctgc acttcgcacc gctggaattc
    13501 ccggctgtcg ctgatttcga atgtacgact gcgctggttg aagctgcgaa atctattggc
    13561 gcgacaactc acgttggcgt gacagtttct tctgatacct tctacccagg tcaggaacgt
    13621 tacgatactt actctggtcg cgtagttcgt cactttaaag gttctatgga agagtggcag
    13681 gcgatgggcg taatgaacta tgaaatggaa tctgcaactc tgctgaccat gtgtgcaagt
    13741 cagggcctgc gtgccggtat ggtagcgggt gttatcgtta accgcaccca gcaagagatc
    13801 ccgaatgctg agacgatgaa acaaaccgaa agccatgcgg tgaaaatcgt ggtggaagcg
    13861 gcgcgtcgtc tgctgtaatt ctcttctcct gtctgaaggc cgacgcgttc ggccttttgt
    13921 atttttgcgt agcgcctcgc aggaaatgcc tttccaactg gacgtttgta cagcacaatt
    13981 ctattttgtg cgggtaagtt gttgcgtcag gaggcgttgt ggatttctca atcatggttt
    14041 acgcagttat tgcgttggtg ggtgtggcaa ttggctggct gtttgccagt tatcaacatg
    14101 cgcagcaaaa agccgagcaa ttagctgaac gtgaagagat ggtcgcggag ttaagcgcgg
    14161 caaaacaaca aattacccaa agcgagcact ggcgtgcaga gtgtgaattg ctcaataacg
    14221 aagtgcgcag cctgcaaagt attaacacct ctctggaggc cgatctgcgt gaagtaacca
    14281 cgcggatgga agccgcacag caacatgctg acgataaaat tcgccagatg attaacagcg
    14341 agcagcgcct cagtgagcag tttgaaaacc tcgccaaccg tatttttgag cacagcaatc
    14401 gccgggttga tgagcaaaac cgtcagagtc tgaacagcct gttgtcgccg ctacgtgaac
    14461 aactggacgg tttccgccgt caggttcagg acagcttcgg taaagaagca caagaacgcc
    14521 ataccctgac ccacgaaatt cgcaatctcc agcaactcaa cgcgcaaatg gcccaggaag
    14581 cgatcaacct gacgcgcgcg ctgaaaggcg acaataaaac ccagggcaac tggggcgagg
    14641 tagtattgac gcgggtgctg gaggcttccg gtctgcgtga agggtatgaa tatgaaaccc
    14701 aggtcagcat cgaaaatgac gcccgctcgc ggatgcagcc ggatgtcatc gtgcggctgc
    14761 cgcagggaaa agatgtggtg atcgacgcca aaatgacgct ggtcgcctat gaacgctatt
    14821 ttaacgccga agacgactac acccgcgaaa gcgcgctaca ggaacatatc gcgtcggtgc
    14881 gtaaccatat ccgtttgctg ggacgcaaag attatcaaca gctgccgggg ctgcgaactc
    14941 tggattacgt gctgatgttt attcccgttg aacccgcttt tttactggcg cttgaccgcc
    15001 agccggagct gatcaccgaa gcgttgaaaa acaatatcat gctggttagc ccgactacgc
    15061 tgctggtggc gttgcgtact attgccaacc tgtggcgtta tgagcatcaa agccgtaacg
    15121 cccagcaaat cgccgatcgt gccagcaagc tgtacgacaa gatgcgtttg ttcatcgatg
    15181 acatgtccgc gattggtcaa agtctcgaca aagcgcagga taactaccgc caggcaatga
    15241 aaaaactctc ttcaggtcgc ggaaatgtgc tggcgcaggc agaagcgttt cgcggtttag
    15301 gagtagaaat taaacgcgag attaatccgg atttggctga acaggcggtg agccaggatg
    15361 aagagtatcg acttcggtcg gttccggagc agccgaatga tgaagcttat caacgcgatg
    15421 atgaatataa tcagcagtcg cgctagccca ttaggggtag ttaagccggg tagaaatcta
    15481 gggcatcgac gcccaatctg ttacacttct ggaacaattt tttgatgagc aggcattgag
    15541 atggtggata agtcacaaga aacgacgcac tttggttttc agaccgtcgc gaaggaacaa
    15601 aaagcggata tggtcgccca cgttttccat tccgtggcat caaaatacga tgtcatgaat
    15661 gatttgatgt catttggtat tcatcgtttg tggaagcgat tcacgattga ttgcagcggc
    15721 gtacgccgtg ggcagaccgt gctggatctg gctggtggca ccggcgacct gacagcgaaa
    15781 ttctcccgcc tggtcggaga aactggcaaa gtggtccttg ctgatatcaa tgaatccatg
    15841 ctcaaaatgg gacgcgagaa gctgcgtaat atcggtgtga ttggcaacgt tgagtatgtt
    15901 caggcgaacg ctgaggcgct gccgttcccg gataacacct ttgattgcat caccatttcg
    15961 tttggtctgc gtaacgtcac cgacaaagat aaagcactgc gttcaatgta tcgcgtgctg
    16021 aaacccggcg gccgcctgct ggtgcttgag ttctcgaagc caattatcga gccgctgagc
    16081 aaagcctatg atgcatactc cttccatgtg ctgccgcgta ttggctcact ggtcgcgaac
    16141 gacgccgaca gctaccgtta tctggcagaa tccatccgta tgcatcccga tcaggatacc
    16201 ctgaaagcca tgatgcagga tgccggattc gaaagtgtcg actactacaa tctgacggca
    16261 ggggttgtgg cgctgcatcg tggttataag ttctgacagg agaccggaaa tgccttttaa
    16321 acctttagtg acggcaggaa ttgaaagtct gctcaacacc ttcctgtatc gctcacccgc
    16381 gctgaaaaca gcccgctcgc gtctgctggg taaagtattg cgcgtggagg taaaaggctt
    16441 ttcgacgtca ttgattctgg tgttcagcga acgccaggtt gatgtactgg gcgaatgggc
    16501 aggcgatgct gactgcaccg ttatcgccta cgccagtgtg ttgccgaaac ttcgcgatcg
    16561 ccagcagctt accgcactga ttcgcagtgg tgagctggaa gtgcagggcg atattcaggt
    16621 ggtgcaaaac ttcgttgcgc tggcagatct ggcagagttc gaccctgcgg aactgctggc
    16681 cccttatacc ggtgatatcg ccgctgaagg aatcagcaaa gccatgcgcg gaggcgcaaa
    16741 gttcctgcat cacggcatta agcgccagca acgttatgtg gcggaagcca ttactgaaga
    16801 gtggcgtatg gcacccggtc cgcttgaagt ggcctggttt gcggaagaga cggctgccgt
    16861 cgagcgtgct gttgatgccc tgaccaaacg gctggaaaaa ctggaggcta aatgacgcca
    16921 ggtgaagtac ggcgcctata tttcatcatt cgcacttttt taagctacgg acttgatgaa
    16981 ctgatcccca aaatgcgtat caccctgccg ctacggctat ggcgatactc attattctgg
    17041 atgccaaatc ggcataaaga caaactttta ggtgagcgac tacgactggc cctgcaagaa
    17101 ctggggccgg tttggatcaa gttcgggcaa atgttatcaa cccgccgcga tctttttcca
    17161 ccgcatattg ccgatcagct ggcgttattg caggacaaag ttgctccgtt tgatggcaag
    17221 ctggcgaagc agcagattga agctgcaatg ggcggcttgc cggtagaagc gtggtttgac
    17281 gattttgaaa tcaagccgct ggcttctgct tctatcgccc aggttcatac cgcgcgattg
    17341 aaatcgaatg gtaaagaggt ggtgattaaa gtcatccgcc cggatatttt gccggtcatt
    17401 aaagcggatc tgaaacttat ctaccgtctg gctcgctggg tgccgcgttt gctgccggat
    17461 ggtcgccgtc tgcgcccaac cgaagtggtg cgcgagtacg aaaagacctt gattgatgaa
    17521 ctgaatttgc tgcgggaatc tgccaacgcc attcagcttc ggcgcaattt tgaagacagc
    17581 ccgatgctct acatcccgga agtttaccct gactattgta gtgaagggat gatggtgatg
    17641 gagcgcattt acggcattcc ggtgtctgat gttgcggcgc tggagaaaaa cggcactaac
    17701 atgaaattgc tggcggaacg cggcgtgcag gtgttcttca ctcaggtctt tcgcgacagc
    17761 tttttccatg ccgatatgca ccctggcaac atcttcgtaa gctatgaaca cccggaaaac
    17821 ccgaaatata tcggcattga ttgcgggatt gttggctcgc taaacaaaga agataaacgc
    17881 tatctggcag aaaactttat cgccttcttt aatcgcgact atcgcaaagt ggcagagcta
    17941 cacgtcgatt ctggctgggt gccaccagat accaacgttg aagagttcga atttgccatt
    18001 cgtacggtct gtgaacctat ctttgagaaa ccgctggccg aaatttcgtt tggacatgta
    18061 ctgttaaatc tgtttaatac ggcgcgtcgc ttcaatatgg aagtgcagcc gcaactggtg
    18121 ttactccaga aaaccctgct ctatgtcgaa ggggtaggac gccagcttta tccgcagctc
    18181 gatttatgga aaacagcgaa gcctttcctg gagtcgtgga ttaaagatca ggtcggtatt
    18241 cctgctctgg tgagagcatt taaagaaaaa gcgccgttct gggtcgaaaa aatgccagaa
    18301 ctgcctgaac tggtttacga cagtttgcgc cagggcaagt atttacagca cagtgttgat
    18361 aagattgccc gcgagcttca gtcaaatcat gtacgtcagg gacaatcgcg ttattttctc
    18421 ggaattggcg ctacgttagt attaagtggc acattcttgt tggtcagccg acctgagtgg
    18481 gggctgatgc ccggctggtt aatggcaggt ggtctgatcg cctggtttgt cggttggcgc
    18541 aaaacacgct gattttttca tcgctcaagg cgggccgtgt aacgtataat gcggctttgt
    18601 ttaatcatca tctaccacag aggaacatgt atgggtggta tcagtatttg gcagttattg
    18661 attattgccg tcatcgttgt actgcttttt ggcaccaaaa agctcggctc catcggttcc
    18721 gatcttggtg cgtcgatcaa aggctttaaa aaagcaatga gcgatgatga accaaagcag
    18781 gataaaacca gtcaggatgc tgattttact gcgaaaacta tcgccgataa gcaggcggat
    18841 acgaatcagg aacaggctaa aacagaagac gcgaagcgcc acgataaaga gcaggtgtaa
    18901 tccgtgtttg atatcggttt tagcgaactg ctattggtgt tcatcatcgg cctcgtcgtt
    18961 ctggggccgc aacgactgcc tgtggcggta aaaacggtag cgggctggat tcgcgcgttg
    19021 cgttcactgg cgacaacggt gcagaacgaa ctgacccagg agttaaaact ccaggagttt
    19081 caggacagtc tgaaaaaggt tgaaaaggcg agcctcacta acctgacgcc cgaactgaaa
    19141 gcgtcgatgg atgaactacg ccaggccgcg gagtcgatga agcgttccta cgttgcaaac
    19201 gatcctgaaa aggcgagcga tgaagcgcac accatccata acccggtggt gaaagataat
    19261 gaagctgcgc atgagggcgt aacgcctgcc gctgcacaaa cgcaggccag ttcgccggaa
    19321 cagaagccag aaaccacgcc agagccggtg gtaaaacctg ctgcggacgc tgaaccgaaa
    19381 accgctgcac cttccccttc gtcgagtgat aaaccgtaaa catgtctgta gaagatactc
    19441 aaccgcttat cacgcatctg attgagctgc gtaagcgtct gctgaactgc attatcgcgg
    19501 tgatcgtgat attcctgtgt ctggtctatt tcgccaatga catctatcac ttggtatccg
    19561 cgccattgat caagcagttg ccgcaaggtt caacgatgat cgccaccgac gtggcctcgc
    19621 cgttctttac gccgatcaag ctgaccttta tggtgtcgct gattctgtca gcgccggtga
    19681 ttctctatca ggtgtgggca tttatcgccc cagcgctgta taagcatgaa cgtcgcctgg
    19741 tggtgccgct gctggtttcc agctctctgc tgttttatat cggcatggca ttcgcctact
    19801 ttgtggtctt tccgctggca tttggcttcc ttgccaatac cgcgccggaa ggggtgcagg
    19861 tatccaccga catcgccagc tatttaagct tcgttatggc gctgtttatg gcgtttggtg
    19921 tctcctttga agtgccggtg gcaattgtgc tgctgtgctg gatggggatt acctcgtcag
    19981 aagacttacg caaaaaacgc ccgtatgtgc tggttggtgc attcgttgtc gggatgttgc
    20041 tgacgccgcc ggatgtcttc tcgcaaacgc tattggcgat cccgatgtac tgtctgtttg
    20101 aaatcggtgt cttcttctca cgcttttacg ttggtaaagg gcgaaatcgg gaagaggaaa
    20161 acgacgctga agcagaaagc gaaaaaactg aagaataaat tcaaccgccc gtcagggcgg
    20221 ttgtcatatg gagtacagga tgtttgatat cggcgttaat ttgaccagtt cgcaatttgc
    20281 gaaagaccgt gatgatgttg tagcgcgcgc ttttgacgcg ggagttaatg ggctactcat
    20341 caccggcact aacctgcgtg aaagccagca ggcgcaaaag ctggcgcgtc agtattcgtc
    20401 ctgttggtca acggcgggcg tacatcctca cgacagcagc cagtggcaag ctgcgactga
    20461 agaagcgatt attgagctgg ccgcgcagcc agaagtggtg gcgattggtg aatgtggtct
    20521 cgactttaac cgtaactttt cgacgccgga agagcaggaa cgcgcttttg ttgctcagct
    20581 acgcattgcc gcagaattaa acatgccggt atttatgcac tgtcgcgatg cccacgagcg
    20641 gtttatgaca ttgctggagc cgtggctgga taaactgcct ggtgcggttc ttcattgctt
    20701 taccggcaca cgcgaagaga tgcaggcgtg cgtggcgcgt ggaatttata tcggcattac
    20761 cggttgggtt tgcgatgaac gacgcgggct ggagctgcgg gaattgttgc cgttgattcc
    20821 ggcggagaaa ttgctgatcg aaacagatgc gccgtatctg ctcccgcgcg atctcacgcc
    20881 aaagccatca tcccggcgca acgagccagc ccatctgccc catattttgc aacgtattgc
    20941 gcactggcgt ggagaagatg ccgcatggct ggctgccacc acggatgcca atgtcaaaac
    21001 actgtttggg attgcgtttt agagtttgcg gaactcggtg ttcttcacac tgtgcttaat
    21061 ctctttatta ataagattaa gcaatagcat ggagcgagcc tcaccatcgg gttcggtgaa
    21121 aatggcctga aagccttcga acgcgccttc ggtaataatc accttatcac ccggataagg
    21181 gggggccgga tcgacaatgt ctttcggttt atataccgat agctgatgaa ttaccgccga
    21241 tgggactatc gctggcgacg cgccaaagcg cacaaagtgg ctgacgccac gcgtagcgct
    21301 gatggtcgtg gtatgaatca cttctggatc aaattccaca aacaggtagt tagggaataa
    21361 cggttcactg actgcggtac gtttaccccg tacgattttt tccagggtga tcatcggagc
    21421 gaggcaatta accgcctgtc tctcaaggtg ttcctgggca cgttgaagtt gcccgcgctt
    21481 gcagtacagt aaataccagg attgcataat gactcttatc cgtttaatcg gggcgcaagg
    21541 atagcaaaag ctttacgcta agttaattat attccccggt ttgcgttata ccgtcagagt
    21601 tcacgctaat ttaacaaatt tacagcattg caaagatgaa cgccgtataa tgggcgcaga
    21661 ttaagaggct acaatggacg ccatgaaata taacgattta cgcgacttcc tgacgctgct
    21721 tgaacagcag ggtgagctaa aacgtatcac gctcccggtg gatccgcatc tggaaatcac
    21781 tgaaattgct gaccgcactc tgcgtgctgg tgggcctgcg ctgttgttcg aaaaccctaa
    21841 aggctactca atgccggtgc tgtgcaacct gttcggtacg ccaaagcgcg tggcgatggg
    21901 catggggcag gaagatgttt cggcgctgcg tgaagttggt aaattattgg cgtttctgaa
    21961 agagccggag ccgccaaaag gtttccgcga cctgtttgat aaactgccgc agtttaagca
    22021 ggtgttaaac atgccgacaa agcgactgcg tggtgcgccc tgccaacaaa aaatcgtctc
    22081 tggcgatgac gtcgatctca atcgcattcc cattatgacc tgctggccgg aagatgccgc
    22141 gccgctgatt acctgggggc tgaccgtaac gcgcggcccg cataaagagc ggcagaatct
    22201 gggcatttat cgccagcagc tgattggtaa aaacaaactg attatgcgct ggctgtcgca
    22261 tcgcggcggc gcgctggatt atcaggagtg gtgtgcggcg catccgggcg aacgtttccc
    22321 ggtttctgtg gcgctgggtg ccgatcctgc cacgattctc ggtgcagtca cccccgttcc
    22381 ggatacgctt tcagagtatg cgtttgccgg attgctacgc ggcaccaaaa ccgaagtagt
    22441 aaagtgtatt tccaatgacc tcgaagtgcc agccagtgcc gaaatcgtcc tggaagggta
    22501 tatcgatcct ggtgagatgg cgccggaagg gccgtatggt gaccacacag gttactataa
    22561 tgaagtcgat aatttcccgg tgtttaccgt gacgcatatt acccagcgtg aagatgcgat
    22621 ttaccattcc acctataccg ggcgtccgcc agatgagccc gcggtactgg gcgtggcgtt
    22681 gaacgaagtg tttgtaccga ttctgcaaaa acagttcccg gaaattgtcg atttttacct
    22741 gccgccggaa ggctgttctt atcgtctggc ggtagtgacg atcaaaaaac agtacgccgg
    22801 acacgcgaag cgcgtcatga tgggcgtctg gtcgttctta cgccagttta tgtacactaa
    22861 atttgtgatc gtttgcgatg atgacgtcaa cgcacgcgac tggaacgatg tgatttgggc
    22921 gattaccacc cgtatggacc cggcgcggga tactgttctg gtagaaaata cgcctattga
    22981 ttatctggat tttgcctcgc ctgtctctgg gctgggttca aaaatggggc tggatgccac
    23041 gaataaatgg ccgggggaaa cccagcgtga atggggacgt cccatcaaaa aagatccaga
    23101 tgttgtcgcg catattgacg ccatctggga tgaactggct atttttaaca acggtaaaag
    23161 cgcctgatgc gcgtttgttt tgccctattt atcgatccga cagagaaagc gcatgacaac
    23221 cttaagctgt aaagtgacct cggtagcagc tatcacggat accgtatatc gtgtccgcat
    23281 cgtgccagac gcggcctttt cttttcgtgc tggtcagtat ttgatggtag tgatggatga
    23341 gcgcgacaaa cgtccgttct caatggcctc gacgccggat gaaaaagggt ttatcgagct
    23401 gcatattggc gcttctgaaa tcaaccttta cgcgaaagca gtcatggacc gcatcctcaa
    23461 agatcatcaa atcgtggtcg accttcccca cggagaagcg tggctgcgcg atgatgaaga
    23521 gcgtccgatg attttgattg cgggcggcac cgggttctct tatgcccgct cgattttgct
    23581 gacagcgttg gcgcgtaacc caaaccgtga tatcaccatt tactggggcg ggcgtgaaga
    23641 gcagcatctg tatgatctct gcgagcttga ggcgctttcg ttaaagcacc ttggtctgca
    23701 agtggtgccg gtggttgaac aaccggaagc aggctggcgt gggcgtactg gtaccgtgtt
    23761 aacggcggta ttgcaggatc atggtacgct ggcagagcat gatatctata ttgccggacg
    23821 ttttgagatg gcgaaaattg cccgtgacct gttttgcagt gagcgtaatg cgcgggaaga
    23881 tcgcctgttt ggcgatgcgt ttgcatttat ctgagatata aaaaaacccg cccctgacag
    23941 gcgggaagaa cggcaactaa aactttattc agtggcattt agatctatgt cgcatccggc
    24001 aattggtgca cgatgcctga tgcgacgctg gcgcgtctta tcaggcctac attggtgccg
    24061 gatcggtaga ccggataagg cgttcacgcc gcatccggca agtggttaaa cccgctcaaa
    24121 caccgtcgca ataccctgac ccagaccgat acacatcgtc gccagaccaa actgaacgtc
    24181 tttgcgttcc atcagattca gcagcgtggt gctgatacgc gcaccggaac aacccagcgg
    24241 atgacccagc gcgatcgcac caccattgag gttgatcttc tcgtcaatct gctccattag
    24301 cccgagatct ttaatgcacg gcaggatctg cgcggcaaag gcttcgttca tctcaaacac
    24361 gccgatatcg ctggcagaaa gccccgcttt tttcagcgcc agcttcgagg ccggaaccgg
    24421 gccgtaaccc ataatcgatg ggtcacaacc aacgaccgcc atcgaacgca cacgagcgcg
    24481 cggcttaaga cctaattcat gggcgcggct ttcactcatc accagcatgg cagctgcgcc
    24541 atcggaaagt gcagaagatg tgcccgccgt taccgtaccg tttactggat caaacgccgg
    24601 acgcagcgtg gcgagggctt ccacggtggt ttccgggcga atcacttcgt cgtaattaaa
    24661 ctgcttcagg acgccgtcgg catcgtgacc accagttggg atgatttcat ttttaaatgc
    24721 ggccgactgc gtggcggccc aggcgcgggc gtgtgaccgc gcggcaaagg catcctgcat
    24781 ttcacggctg ataccgtgca tacgcgccag catttctgcc gttaagccca tcatgcccgc
    24841 cgctttggcg acattgcggc tcaggccggg gtgaaaatcg acgccgtgac tcatcggcac
    24901 atggcctata tgctccacgc cgccaaccag acatgcctgc gtatcgccag tcatgatcat
    24961 tcgtgctgcg tcatgcagtg cctgcatgga tgaaccacac aagcgattga cggtaaccgc
    25021 cgggacagag tgcggcactt ctgccagcag cgccgcgtta cgggcgatgt taaaaccctg
    25081 ctccagcgtc tgttgcacac aaccccagta aatatcgtcg agggccgccg cttccagcgc
    25141 cgggttacgc gccagcaggc tacgcattaa atgagcggag agatcttccg cacgcacgtt
    25201 acgaaaagca ccgcccttcg aacggcccat cggggtgcga attgcatcga caatgacaac
    25261 ctgttccatt gtgactcctt aagccgtttt caggtcgcca accggacggg ctggctcaac
    25321 cggaggatag tacggttcgt tatgacgcgc tttattacgc agaccttccg gcacttcata
    25381 cagcgggccg aggtgctgat attgctgtgc catatcgagg tattttgcgc taccgagggt
    25441 gtccagccag cggaacgcgc cgccgtggaa cggagggaag cccaggccgt agaccagcgc
    25501 catatccgct tccgccggag tggcgataat gccctcttcc agacagcgca ccacttcgtt
    25561 gaccatcggg atcatcatgc gggcgataat ctcttcttcg ctgaaatcgc gcttcggttg
    25621 gctcacttct gccagcaggt cttcaacggc ggcgtcttct tctttcttcg gcttaccttt
    25681 gctgtcttct ttataacgcc agaaaccgag gccgttcttc tgaccaaagc ggttggcatc
    25741 aaacagcgcg tcgatggcat cgcggtaatc tttctgcatc cgctgcggga agcctgctgc
    25801 catgacagcc tgagcgtgat gcgcggtatc aatgcccaca acgtccagca gatatgccgg
    25861 gcccatcggc cagccaaact gtttttccat cactttgtcg atcttgcgga aatccgcgcc
    25921 gtcgcgcagc agctggctga aaccggcgaa atacgggaac agcacgcggt taacaaagaa
    25981 gccggggcag tcgttaacca caatcggcgt cttgcccatc ttgctcgccc aggcgacaac
    26041 tttcgcgatg gtttcgtcgg agcttttctc gccgcgaata atttctacca acggcattcg
    26101 gtggaccggg ttaaagaagt gcatcccgca gaagttttcc gggcgttcca gcgcgttggc
    26161 cagttcgctg ataggaatgg ttgaagtgtt agacgccagc acggtatcct ggcgtacttt
    26221 ttgttcggtt tctgccagta cggctttttt cactttcggg ttttcaacaa ccgcttctac
    26281 cacaatatcc acgcggtcaa atccggcgta gtcgagcgtt gggtggatcg tggagatcac
    26341 gccagccagt ttcagaccat cgattttgcc gcgctcaagc tgcttgttca gcagtttcgc
    26401 ggcttcggtc atgccgaggg ttaacgactt gtcgttgata tctttcatga caaccggcac
    26461 gcctttccac gcggactggt aagcgatacc gccacccatg atgcctgcgc ccagcacagc
    26521 agcctgtttc ggggtttcaa cgtctttggt gagtttcttc gctttgcctt ttacatattg
    26581 atcgttaagg aaaatgccga ccagtgcgcg agcttcgttg gtatgcgcca gcgggacaaa
    26641 acttttgttt tccagattta aggcttcttc acgaccaaaa cgggccgcag cttcaatggt
    26701 ttttactgcg gtgatggggg ccggataatg tttccccgcc gtttgcgcga ccattccttt
    26761 agcgatggtg aagctcatgg tggcttcaat cttgctcagt tttagcggtt cctgcttcgg
    26821 ctgacgtttc gctttccagt cgaggtcgcc gttaatggcc tggcgtaaaa tcgccatcgc
    26881 cccttcaatc agtttttctg ctttgactac gccatccacc agaccgattt tcagcgcctg
    26941 atccgcgccg acatctttac cggcggcaat gatttccagt gcactgtcag cgcccagcat
    27001 acgtggcata cgtacagaac cgccaaagcc cggcatgatg cccagtttgg tttccggcag
    27061 accgatgcgc agatccggcg tcgccagacg ataatcggtc gccagcacgc attcgcagcc
    27121 accacccagc gcatagccgt tgacggcagc aatggtcggc accggcagat cttccaggcg
    27181 attaaacacg ctattggcaa agtgcagcca ctgacttaac tgttcttcag gaacgaggaa
    27241 cagggacaaa aattcggtga tatcagcacc gacgataaag gctgctttgt tcgaacgcag
    27301 cagcagccct tttagatctg attgctgttc cagcacgccg atggcctcgc cgaggctggc
    27361 gacggtcgca gtgtcgagtt tattaactga acctggggca tcaaatacca gttcggcaat
    27421 gccatcttcc agccagtcaa ggtacagggt gtcgcctttg taaagcatgt cagtctcctg
    27481 aatccgcaag gtaatctggt cgtaccagat gcatcggagt gtgtgttttg tgttaaaaat
    27541 atgcaaataa aagattaaag aaatgccgat ctgatcacgc tcggcagaaa tcacgctctg
    27601 gatgaacgat gtgctaagat gcggagactt aaggtcaaaa aaacagaagg gtaaaaaatg
    27661 gaatcactgg cctcgctcta taaaaatcat atagctacct tacaagaacg gactcgcgat
    27721 gcgctggcgc gcttcaagct ggatgcatta cttattcact ccggcgagct gttcaacgtt
    27781 tttctcgacg atcatcccta tccgtttaaa gtgaacccgc aattcaaagc gtgggtgccg
    27841 gtaactcagg tgccaaactg ctggttgctg gtggatggcg tgaacaagcc gaaactgtgg
    27901 ttctatctgc cggttgatta ctggcacaac gtcgaaccgc tgccgacctc cttctggact
    27961 gaagatgtgg aagtgatcgc gctgccgaaa gccgatggca ttggtagcct gctgcctgct
    28021 gcgcgcggca atatcggtta tatcggtccg gtgccggaac gtgcgctgca actgggtatt
    28081 gaggccagca acatcaaccc gaaaggggtg atcgactacc tgcattatta ccgctccttc
    28141 aaaaccgagt acgaactggc ctgtatgcgt gaagcgcaga aaatggcggt caatggtcat
    28201 cgtgcggcag aagaagcgtt ccgttctggc atgagcgagt tcgatatcaa tattgcctat
    28261 ctgaccgcga ccggtcatcg tgataccgac gtaccttaca gcaacattgt ggcgcttaac
    28321 gaacacgctg cggtgttgca ttacaccaaa ctggaccacc aggcaccgga agagatgcgc
    28381 agcttcctgc tggatgccgg ggccgaatat aacggctatg cggctgacct gacccgtacc
    28441 tggtcggcaa aaagcgacaa cgactacgca cagctggtga aagacgtaaa tgatgaacaa
    28501 ctggcgctga tcgccaccat gaaagcaggc gtcagctatg tggattacca catccagttc
    28561 catcagcgca tcgccaaact gctgcgtaaa catcaaatca tcaccgatat gagtgaagaa
    28621 gcgatggtcg aaaacgatct caccggaccg tttatgccgc acggtattgg ccatccgctg
    28681 ggcctgcagg tgcatgacgt cgccggtttt atgcaggatg atagcggtac acacctcgcg
    28741 gcaccggcaa aatatccgta cctgcgctac acccgtattc tccagccggg catggtgtta
    28801 accatcgaac cgggtatcta cttcattgaa tcgcttctgg caccgtggcg tgaagggcag
    28861 ttcagcaagc acttcaactg gcagaaaatt gaagcactga aaccgttcgg cggcattcgt
    28921 atcgaagaca acgtggtgat ccacgaaaac aacgtggaaa acatgacccg ggatctgaaa
    28981 ctggcgtgat ggaaagctgg ttaattcctg cggcaccggt cacggtcgtt gaagagatca
    29041 aaaagagccg cttcataacg atgttggcgc ataccgatgg cgttgaggcg gcgaaagcgt
    29101 ttgttgaatc ggtgcgggca gaacaccccg atgcccgcca ccattgcgtg gcgtgggtgg
    29161 cgggtgcgcc ggatgattct caacagctgg gtttctctga cgacggggag ccggcgggaa
    29221 cggcaggtaa accgatgctc gcccagctaa tgggcagcgg cgtcggggaa attaccgctg
    29281 tggtagtgcg ctactacggc ggcatattgc ttggcaccgg tgggttagtg aaagcgtatg
    29341 gcggcggcgt gaatcaggcg ctgcgccagc taacgaccca acgcaagacg ccattaaccg
    29401 aatatacttt gcaatgtgaa tatagtcagt taaccggcat tgaagcgttg ctggggcagt
    29461 gtgacggcaa aattatcaac agtgattatc aggcattcgt tctgctgcgg gtggcgcttc
    29521 cggcggcgaa agtggctgaa ttttccgcaa agctggcgga ttttagccgt ggttcattgc
    29581 aattgttagc gattgaagaa taatccccac ttcgttttgc agaactaagg aagcggcaga
    29641 gatgcatttt cgcgccatta cccgaatcgt tggactactg gtcatcttat tttcagggac
    29701 catgattatc cctgggctgg tagcactcat ctaccgggat ggagcgggcc gcgcttttac
    29761 ccagaccttt tttgtcgccc tcgccattgg ctctatgctg tggtggccga accgcaaaga
    29821 gaagggcgaa ctgaaatccc gtgaggggtt tctgatagtg gtgctgtttt ggaccgtgct
    29881 gggtagtgtc ggtgcgctcc cttttatctt ctcggaaagc ccgaacctca cgattaccga
    29941 tgcgtttttt gaatctttct ctggcctgac caccaccggg gccactacgc tggtggggct
    30001 ggattcgctc cctcatgcca tcctctttta tcgccagatg ctgcaatggt ttggcgggat
    30061 ggggatcatc gtgttagcgg ttgcgatact gcctatcctc ggcgtgggtg ggatgcagct
    30121 ctatcgcgca gaaatgcccg gcccgctgaa agataacaaa atgcgcccgc gaattgcgga
    30181 aacggcgaaa actctgtggt tgatttatgt cttgctgacc gtcgcctgtg cgctggcgtt
    30241 gtggtttgcc ggaatggatg cctttgatgc catcggccat agctttgcga ctatcgctat
    30301 tggcggcttc tcgacacatg atgccagtat cggttatttc gacagtccga ctattaacac
    30361 tatcattgct atcttcctgc tgatctccgg ctgtaactac ggtctgcact tttcactgtt
    30421 aagtgggcgt agtctgaagg tttattggcg cgatccggaa tttcgcatgt ttatcggcgt
    30481 acagtttacg ctggtggtta tttgtacact cgtactgtgg tttcataatg tctacagttc
    30541 ggcgctgatg acaattaacc aggcgttttt ccaggtggta tcgatggcga caaccgccgg
    30601 gtttacgact gacagcattg cccgctggcc gctctttttg ccggtactgc ttttatgttc
    30661 agcttttatc ggcggttgcg ccgggtcaac gggcggtggc ctgaaagtga tccgcatcct
    30721 gctgctgttt aagcagggga accgtgagct gaaacgactg gtgcatccga acgccgtcta
    30781 tagcattaag ctggggaatc gcgcactgcc ggaacgtatc ctcgaagccg tttggggatt
    30841 tttctccgcc tatgcattgg tgtttattgt cagtatgctg gcgattatcg ccacgggcgt
    30901 ggatgatttt tctgcctttg cctcagttgt tgcgacattg aataacctgg ggccagggct
    30961 tggcgtggtt gctgataact ttaccagtat gaacccggtg gctaaatgga tcctgattgc
    31021 caacatgctg tttggtcgtc tcgaggtctt tacattactg gtgctcttta ccccgacttt
    31081 ctggcgtgaa tgatggagta atacgtgaaa acattaattc ttttctcaac aagggacgga
    31141 caaacgcgcg agattgcctc ctacctggct tcggaactga aagaactggg gatccagacg
    31201 gatgtcgcca atgtgcaccg cattgaagaa ccacaatggg aaaactatga ccgtgtggtc
    31261 attggtgctt ctattcgcta tggtcactac cactctgcgt tccaggaatt tgtcaaaaaa
    31321 catgcgacgc gactgaattc gatgccgagc gccttttatt ccgtaaacct ggtggcgcgc
    31381 aaaccggaga agcgtactcc acagaccaac agctacgcgc ggaagtttct gatgaactcg
    31441 caatggcgtc ccgatcgctg cgcggtcatt gccggggcgc tgcgttaccc acgttatcgc
    31501 tggtacgacc gttttatgct caagctgatt atgaagatgt caggcggtga aacggatacg
    31561 cgcaaagaag ttgtctatac cgattgggag caggtggcga atttcgcccg agaaatcgcc
    31621 catttaaccg acaaaccgac gctgaaataa gcataaagaa taaaaaatgc gcggtcagaa
    31681 aattatttta aatttcctct tgtcaggccg gaataactcc ctataatgcg ccaccactga
    31741 cacggaacaa cggcaaacac gcagccgggt cagcggggtt ctcctgagaa ttccgacaga
    31801 gaaagcaaaa ataaatgctt gactttgtag cgggaaagcg tattatgaac accccgcgcc
    31861 gctgagaaaa agcgaagcgg cactgctctt taacaattta tcagacaatc tgtgtgggca
    31921 ctcgaagata cggattctta acgtcgcaag acga
//
LOCUS       DAZPCS010000030        31186 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZPCS010000030 DAZPCS010000000
VERSION     DAZPCS010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31186
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.030"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            389..2047
                     /locus_tag="ACHUQ7_001397"
     CDS             389..2047
                     /locus_tag="ACHUQ7_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFR1134879.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2044..3000)
                     /locus_tag="ACHUQ7_001398"
     CDS             complement(2044..3000)
                     /locus_tag="ACHUQ7_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFR1134880.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3151..4266
                     /gene="macA"
                     /locus_tag="ACHUQ7_001399"
     CDS             3151..4266
                     /gene="macA"
                     /locus_tag="ACHUQ7_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFR1134881.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4263..6209
                     /gene="macB"
                     /locus_tag="ACHUQ7_001400"
     CDS             4263..6209
                     /gene="macB"
                     /locus_tag="ACHUQ7_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFR1134882.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLNIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDIATLDADALAQLRREHFGFIFQRYHLLPHLT
                     VEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6282..6506)
                     /gene="cspD"
                     /locus_tag="ACHUQ7_001401"
     CDS             complement(6282..6506)
                     /gene="cspD"
                     /locus_tag="ACHUQ7_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFR1134883.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6656..6730
                     /gene="yljB"
                     /locus_tag="ACHUQ7_001402"
     CDS             6656..6730
                     /gene="yljB"
                     /locus_tag="ACHUQ7_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFR1134884.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            6829..7149
                     /gene="clpS"
                     /locus_tag="ACHUQ7_001403"
     CDS             6829..7149
                     /gene="clpS"
                     /locus_tag="ACHUQ7_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFR1134885.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7180..9456
                     /gene="clpA"
                     /locus_tag="ACHUQ7_001404"
     CDS             7180..9456
                     /gene="clpA"
                     /locus_tag="ACHUQ7_001404"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFR1134886.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     KLSDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9859..9946)
                     /locus_tag="ACHUQ7_001405"
     tRNA            complement(9859..9946)
                     /locus_tag="ACHUQ7_001405"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9910..9912),aa:Ser,seq:gga)
     gene            complement(10200..10418)
                     /gene="infA"
                     /locus_tag="ACHUQ7_001406"
     CDS             complement(10200..10418)
                     /gene="infA"
                     /locus_tag="ACHUQ7_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFR1134887.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10703..11407)
                     /gene="aat"
                     /locus_tag="ACHUQ7_001407"
     CDS             complement(10703..11407)
                     /gene="aat"
                     /locus_tag="ACHUQ7_001407"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFR1134888.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(11449..13170)
                     /gene="cydC"
                     /locus_tag="ACHUQ7_001408"
     CDS             complement(11449..13170)
                     /gene="cydC"
                     /locus_tag="ACHUQ7_001408"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFR1134889.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARTLLHDAPLVLLDEPTEGLDAT
                     TESQILELLAKMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13171..14937)
                     /gene="cydD"
                     /locus_tag="ACHUQ7_001409"
     CDS             complement(13171..14937)
                     /gene="cydD"
                     /locus_tag="ACHUQ7_001409"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFR1134890.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(15060..16025)
                     /gene="trxB"
                     /locus_tag="ACHUQ7_001410"
     CDS             complement(15060..16025)
                     /gene="trxB"
                     /locus_tag="ACHUQ7_001410"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFR1134891.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16569..17063
                     /gene="lrp"
                     /locus_tag="ACHUQ7_001411"
     CDS             16569..17063
                     /gene="lrp"
                     /locus_tag="ACHUQ7_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFR1134892.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            17198..21226
                     /gene="ftsK"
                     /locus_tag="ACHUQ7_001412"
     CDS             17198..21226
                     /gene="ftsK"
                     /locus_tag="ACHUQ7_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFR1134893.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNSAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQQQYQQPQQQVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLAPPPFD"
     gene            21381..21992
                     /gene="lolA"
                     /locus_tag="ACHUQ7_001413"
     CDS             21381..21992
                     /gene="lolA"
                     /locus_tag="ACHUQ7_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFR1134894.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            22003..23346
                     /gene="rarA"
                     /locus_tag="ACHUQ7_001414"
                     /gene_synonym="mgsA"
     CDS             22003..23346
                     /gene="rarA"
                     /locus_tag="ACHUQ7_001414"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFR1134895.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23437..24729
                     /gene="serS"
                     /locus_tag="ACHUQ7_001415"
     CDS             23437..24729
                     /gene="serS"
                     /locus_tag="ACHUQ7_001415"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFR1134896.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24969..27412
                     /gene="dmsA"
                     /locus_tag="ACHUQ7_001416"
                     /pseudo
     CDS             24969..27412
                     /gene="dmsA"
                     /locus_tag="ACHUQ7_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            27423..28040
                     /gene="dmsB"
                     /locus_tag="ACHUQ7_001417"
     CDS             27423..28040
                     /gene="dmsB"
                     /locus_tag="ACHUQ7_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFR1134897.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            28042..28833
                     /locus_tag="ACHUQ7_001418"
     CDS             28042..28833
                     /locus_tag="ACHUQ7_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0009390 - dimethyl sulfoxide reductase
                     complex [Evidence IEA];
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     family protein"
                     /protein_id="HFR1134898.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGWAMRLL
                     PAISVLALVVSGVMSVMQGTELATIHSSVQQAAALVPDYGALMSWRIVLLAVALCLWI
                     APQLKGYQPAVPLLSVSFILLLAGELIGRGVFYGLHMTVGMAVAS"
     gene            complement(28869..29495)
                     /gene="ycaC"
                     /locus_tag="ACHUQ7_001419"
     CDS             complement(28869..29495)
                     /gene="ycaC"
                     /locus_tag="ACHUQ7_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFR1134899.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVLELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29810..30958
                     /locus_tag="ACHUQ7_001420"
     CDS             29810..30958
                     /locus_tag="ACHUQ7_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1134900.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFSAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            30976..>31186
                     /locus_tag="ACHUQ7_001421"
     CDS             30976..>31186
                     /locus_tag="ACHUQ7_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001345656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR1134901.1"
                     /translation="MNLLFHTLLFCFFYLTCFFPNFIRLKIKCAAGYNFAFRYFRTSY
                     LPRIIPRFTDAPLILAKDLSMAGNVQ"
BASE COUNT         7689 a         7644 c         8374 g         7479 t
ORIGIN      
        1 tggtcgtttt atagggtcgt cgtaaaaaat gacgacggaa ataatgcgcg gctattttaa
       61 aaacgaaggc gagtcattca ccagataaat aaatccagta aatttgattt agggcaacag
      121 cgggttgccc catatagtca tttgtctgat tgacagtgta gtgcaagcaa aagatttaat
      181 cctttaggcg taataaaaaa taatttatca tgctaattat ttgattttgt tgtttttgcg
      241 ggcttatcag caagagggag tataacgcga ttattcgctc atttttcaga caattgccat
      301 gcttaaatgt gatgtcatca cgtattagca aggcctttcc cgttatactg cgagcgtaaa
      361 ggataagtca catatttctg gaggggatat gattcttgag cgcgttgaaa ttgtgggttt
      421 tcgcggtatc aaccgtttgt cgttgatgct cgaacaaaac aacgtcctga ttggggagaa
      481 cgcgtggggt aaatccagct tgctggacgc cttaaccctg ctgctatcgc cagaatcaga
      541 tctctaccat tttgagcgcg acgatttctg gttcccgccg ggagatatca acgggcgaga
      601 acatcatctg catattattt tgaccttccg cgaatcgctg ccaggccgac atcgggttcg
      661 ccgttatcgg ccgctggaag cgtgctggac gccatgcacc gatggctatc accgtatttt
      721 ttatcgtctg gaaggggaga gtgcggaaga cggcagcgtg atgacactgc gcagttttct
      781 cgataaagac ggacatccga ttgatgttga ggatattaac gatcaggcac gccatctggt
      841 gcgtttaatg ccggtgctgc gcttgcgtga tgcccgtttt atgcgccgta ttcgtaacgg
      901 cacggtgcca aatgtcccca atgtggaagt caccgcgcgc cagctcgatt tcctcgcccg
      961 tgagttgtcc tcacatccgc aaaatctctc tgatggacag attcgtcagg gactttccgc
     1021 aatggtgcag ttgcttgagc attatttctc tgagcagggg gctggacagg cgcgatatcg
     1081 tttaatgcgg cggcgggcca gcaatgagca acgaagctgg cgctatctgg acatcatcaa
     1141 ccggatgatt gaccgacctg gagggcgctc gtatcgggtt attttgctcg gcctgtttgc
     1201 tactttattg caggcaaaag gcacattgcg actggataaa gacgcgcgtc cgttattgct
     1261 aattgaagat ccagaaaccc gcctgcatcc gattatgctt tccgttgcct ggcatctgtt
     1321 gaatcttttg cctttacagc gtattgctac caccaactct ggcgagctgc tttcgttaac
     1381 tccagtggaa catgtttgcc gcctggtgcg tgaatcttct cgtgttgccg cctggcgtct
     1441 ggggccaagt ggcttgagca ccgaagacag ccgccgcatc tcttttcaca ttcgttttaa
     1501 tcgtccgtca tcgctgtttg ctcgctgctg gttgctggtg gaaggggaaa cggaaacctg
     1561 ggttatcaat gaactggcgc gtcagtgcgg acatcatttt gatgccgaag ggatcaaggt
     1621 cattgagttt gcccagtccg ggctaaagcc actggttaaa tttgcccgcc gaatggggat
     1681 tgaatggcat gtactggtcg atggcgatga agcagggaag aaatatgccg ctacggtacg
     1741 cagcctgttg aataacgatc gggaagccga acgagaacat ttaacggcgt taccggcgct
     1801 ggatatggaa cattttatgt atcgccaggg attttccgat gtgttccacc gcgtggcgca
     1861 aatcccggaa aatgtaccga tgaatctacg caaaattatc tcgaaagcga tccatcgctc
     1921 ttccaaaccc gatcttgcca ttgaagtggc aatggaggca ggacgtcgtg gtgtggactc
     1981 cgtaccaacg ctgctgaaaa aaatgttctc acgcgtgctg tggctggcgc gcggtcgcgc
     2041 ggattaaccg cgaaacatcg tggccatttg tggctgaata gcgtcgagca tctcatagcg
     2101 ccgacggtat tcagcccgtt ttttactggc gatttcggca atctcttttc gtgctatctg
     2161 tgctggaagg cggtaatggc gttcagcatc acatacgccg ccaaccgact cccagaacgc
     2221 gttgtaatca gcatggatct tgccttcttt atcgcgataa cgcaggctgc ggtaaatatg
     2281 cgtttcattg ctgacggcaa taatctgctc tacctgcaaa cgttgggcaa acagacaggc
     2341 cgcttccatc acgaggcgtt tgggaaatag cccgtggcag gctttcgtcg cattctggat
     2401 ttcctgatgt ggaatttccc attttgcgcc ttgcagtccg ccaataaaca tcgttctttt
     2461 cccctgatat tcacacaggg taaacgtgat ctctgccaga ggaatacctt cgctgttgcg
     2521 gaacaggatt gtgctgtcac cttctttatc cattgagatc atcatggtca gctcaagcgt
     2581 gaactgctca ccgtttttgc cttccagttt agccagttgc agcccggggg tattcaaata
     2641 taagctgaat tcttccgccg acatacaccc gcggagtaac gcataatggt aacgtaacgc
     2701 ctccagcaat tgcttacggc taagattcgc ggcaaggtaa gggcgatgca gacgcacagg
     2761 cagtcgcggc tggcgcgtta acaacacatt gagattaggc cagtgggaaa gttcgttcat
     2821 ccactcaacg cttaaacgcg gcataatcaa cgagcgcagc aaaaatttct ggcgaaaact
     2881 acagcgatgc cagaatttac ccggccgaca ctgtccacgt gccagactaa gaaaaagtga
     2941 caggctgctg agagattcag atggcgtaaa ggtccgttca gttagctgcg acatattcat
     3001 gaaatcaatg gttatacatg acgtcgattt caccattgcg tatcttaacc aaacatcaat
     3061 agtgtgatta ctaacgtaaa ttttagggtt ttgttgatat ttcgttgaag ttaatgaccc
     3121 ggattggcat atggagtatt cagaatattt atgaaaaagc ggaaaaccgt gaagaagcgt
     3181 tacgttattg cgctggtgat agtcatcgcc ggactgatta cgttatggag aattcttaac
     3241 gcacccgtgc cgacttatca gacactgatt gtgcgccccg gtgatttaca gcaaagcgtg
     3301 ctggcgaccg gaaagctgga cgcgctgcgt aaggttgacg tgggcgcgca ggtcagcggt
     3361 cagttgaaaa cgctgtcggt ggcgattggc gataaagtaa aaaaagacca gcttttaggg
     3421 gtcattgatc ctgaacaggc tgaaaaccag atcaaggagg tcgaagcaac gctgatggag
     3481 ctacgcgcgc agcggcagca ggcggaagcg gagctgaaac tggcgcgggt gacgtattcc
     3541 cgtcagcaac gtctggcaca aacgcaagcg gtttcactgc aggatctcga taccgccgcg
     3601 acggagatgg ctgtgaaaca ggcgcaaatt ggcaccattg acgcgcaaat caagcgcaat
     3661 caggcctctc tcgatacggc taaaaccaat ctcgattaca cccgtatcgt tgccccgatg
     3721 gctggggaag tgacgcaaat caccactctg caaggccaga cggtgattgc cgcacagcaa
     3781 gcaccgaaca ttctgacgct ggcagatatg agcactatgc tggtaaaagc gcaggtttct
     3841 gaagcggatg taatccacct gaagccgggg caaaaagcct ggtttacggt gcttggcgat
     3901 ccactgacgc gctacgaggg gcaaatcaag gatgtgctac cgacgccgga aaaggtaaac
     3961 gacgctattt tctattacgc ccgttttgaa gtacccaacc ccaatggttt gctgcggctg
     4021 gatatgaccg cgcaagtgca tattcagctc accgatgtga aaaatgtgtt gacgatccct
     4081 ctgtcggcgt taggcgatcc ggttggcgat aatcgttata aagtcaaatt gttgcgtaat
     4141 ggtgaaacac gcgagcgtga agtgacgatt ggcgcacgta acgataccga tgttgagatt
     4201 gtcaaagggc tggaagcggg cgatgaagtg gtgattggtg aggccaaacc aggagctgca
     4261 caatgacgcc tttgctcgaa ttaaaggata ttcgtcgcag ctatcctgcc ggtgatgagc
     4321 aggttgaggt gctgaagggc atcagcctca atatttatgc gggtgagatg gtcgcgattg
     4381 ttggcgcttc gggttccggt aaatcgaccc tgatgaatat tctcggctgc ctggataagg
     4441 ccaccagcgg cacctatcgc gtagccggtc aggatattgc cacgctggac gccgatgcgc
     4501 tggcgcaact gcgccgcgag catttcggct ttattttcca gcgttaccat ttgcttccgc
     4561 atttaaccgt cgagcagaac gttgaagtac ccgccgtcta tgccggtctt gagcggaaac
     4621 agcgactgct tcgtgcccag gagttgctgc aacggctggg gctggaagac cgtacagagt
     4681 attatccggc acagctttcg ggtggtcagc aacagcgcgt cagcatcgcg cgggcattga
     4741 tgaacggcgg tcaggtaatt cttgccgatg aaccgaccgg cgcactggac agccattctg
     4801 gcgaagaggt gatggcgatc ctgcatcagc tgcgcgatcg cgggcatacg gtgattatcg
     4861 tcacccacga tccgcaggtc gctgctcagg ccgagcgggt gatcgaaatt cgcgacggcg
     4921 aaattgtgcg caatcctccc gccattgaaa aagtgaatgt tgctggcggg acggagcctg
     4981 ttgtcaatac agtgtctggc tggcggcagt ttgtcagcgg ttttaacgag gcgctgacga
     5041 tggcatggcg ggcgctggca gcgaataaaa tgcgtacttt actgaccatg ctggggatta
     5101 ttatcggtat tgcgtcggtg gtttccattg tcgtggtggg tgacgccgcc aaacaaatgg
     5161 tgctggcgga tattcgttct attggtacga atactattga tgtctatccc gggaaagatt
     5221 ttggcgatga cgatccgcaa tatcagcagg cgctgaagta cgacgactta atcgccatcc
     5281 aaaaacaacc gtgggtcgcc tcagccacac ctgccgtctc gcaaaacctg cgcctgcgtt
     5341 ataacaatgt tgatgttgct gccagtgcca atggcgtgag cggcgattat tttaatgtct
     5401 atggcatgac cttcagtgaa ggaaacacct ttaatcagga gcagctgaac ggtcgtgcgc
     5461 aggtcgtggt tctcgacagt aatactcgcc gccagctttt cccccataaa gcagatgtgg
     5521 ttggcgaggt gattctggtc ggcaatatgc ccgccagagt cattggtgtg gcggaagaaa
     5581 aacagtcgat gtttggtagc agtaaagtgc tgcgtgtctg gctaccttac agcacgatgt
     5641 ccgggcgagt tatgggccag tcgtggctta actccattac tgtcagggtg aaagaaggat
     5701 ttgacagcgc cgaggcggaa cagcaactca cgcgtttact ttcactgcgc cacggtaaga
     5761 aggatttctt tacctggaac atggacggcg tcttgaaaac tgttgaaaag accacacgta
     5821 ctttacaact gtttctgacg ctggtggcgg tgatttcgct ggtggtgggc ggtattggtg
     5881 taatgaatat tatgctggtg tcagtgaccg agcggacgcg ggaaattggc attcgcatgg
     5941 ctgtaggtgc gcgagcaagc gatgttttgc aacagttcct gatcgaagcc gtactggttt
     6001 gcctggtcgg tggcgcgttg ggaataacac tgtcactgtt aattgctttc accttgcagc
     6061 ttttcttacc cggctgggag attggttttt caccgttggc gctgctgctg gcgtttctct
     6121 gctcgacggc caccgggatt ttatttggct ggttacccgc acgaaatgcg gcacgactgg
     6181 atccagtaga tgctctggca cgggagtaat ttttgagata aaaatgccag ccgatcgggc
     6241 tggcattttg cctttaggat gtacacaatg agacagaaga gctatgcgac tgccgcttct
     6301 acttcgacgg gcacaataac actggcgtga ttgccttttg gcccctggtg gacatcaaac
     6361 tgaacggatt gtccagcttt tagcgttctg taaccatcca tctgaatggt ggaataatga
     6421 gcgaaaatat cttcgccgcc gccttcaggg cagatgaaac caaacccttt ggcattgttg
     6481 aaccacttaa cagtaccctt ttccatgctt cgacatcctt cgcaaatctt atacaagtaa
     6541 gatggaataa accggggtca gagagggggc tgttcaaaac ctcgccaact ctagaaatac
     6601 aatttagaga attagggcga gccgtcaagc atttgacagg ggccaggggg caggtatgaa
     6661 tcaaaaattt gaagcagtta acgctattga caggaatgtg acagatgtcg ctgatgccaa
     6721 cgatagatga tagttatcta tcatgtggag tagattggtc aggcaaataa gctcttgtca
     6781 gcggcagggc gttctgccga taaccgtaac cgaagatgat aactgacaat gggtaaaacg
     6841 aacgactggc tggactttga tcaactggcg gaagaaaaag ttcgcgacgc gctaaaaccg
     6901 ccatctatgt ataaagtgat attagtcaat gatgattaca ctccgatgga gtttgttatt
     6961 gacgtgttac aaaaattctt ttcttatgat gtagaacgtg caacgcaatt gatgctcgct
     7021 gttcactacc aggggaaggc catttgcgga gtctttaccg ccgaggttgc agaaaccaaa
     7081 gtggcgatgg tgaacaagta cgcgagggag aatgagcatc cattgctgtg tacgctagaa
     7141 aaagcctgaa tgcaggcata aaaattgggg gaggtgccta tgctcaatca agaactggaa
     7201 ctcagtttaa atatggcttt cgccagagcg cgcgagcacc gtcatgagtt tatgaccgtc
     7261 gagcacttgt tactggcgct gctcagtaac ccatctgccc gggaggcgct ggaagcgtgt
     7321 tctgtggatt tggttgcgct ccgtcaggaa ctggaagcct ttattgaaca aaccacaccc
     7381 gttctgcctg ccagtgaaga ggagcgcgac acacagccga cgctgagttt tcagcgtgta
     7441 ctgcaacgtg cggtcttcca tgtccagtcc tccggtcgca atgaggttac cggtgcaaac
     7501 gttctggtcg ctatcttcag cgaacaggag tcgcaggcgg catatctgtt gcgtaaacat
     7561 gaagtcagcc gtctcgatgt ggtgaacttt atctctcatg gcacgcgtaa agacgagccg
     7621 acacagtctt ctgatcctgg cagccagcca aacagcgaag aacaagctgg tggggaggaa
     7681 cgtatggaga atttcacgac gaacctgaac cagcttgcgc gtgtgggcgg aatcgaccca
     7741 ctaattggtc gtgagaaaga gctggagcgt gctattcagg ttctctgccg tcgccgtaaa
     7801 aacaacccgc tgctggtggg ggaatctggt gtcggtaaaa ccgcgattgc agaaggtctt
     7861 gcctggcgaa ttgttcaggg tgatgtgccg gaagtgatgg ctgactgtac gatttactct
     7921 ctcgatatcg gttctctgtt agcgggcaca aaatatcgcg gcgactttga aaaacgtttt
     7981 aaagcgttgc tcaagcagct ggagcaggac actaacagca tcctgtttat tgatgagatc
     8041 cacaccatta tcggtgcggg tgcagcgtct ggtggtcagg tcgatgcggc taacctaatc
     8101 aaaccgttgc tctccagcgg taaaattcgc gtaattggtt cgacaaccta tcaggagttc
     8161 agcaacattt tcgagaaaga ccgtgctctg gcgcgtcgct tccagaaaat tgatattact
     8221 gaaccgtcga tcgaagaaac tgttcaaatc atcaatggcc tgaaaccgaa gtatgaagcg
     8281 caccacgacg tgcgttatac cgcaaaagcg gtgcgtgcgg cggtagagct ggcggtgaaa
     8341 tacattaacg atcgtcatct gccggataaa gccattgatg ttatcgacga agcaggcgct
     8401 cgcgcacgtc tgatgccggt aagcaaacgc aagaaaaccg ttaatgtggc ggatattgag
     8461 tccgtggtgg cccgtattgc acgcattcca gagaagagtg tttctcagag tgaccgcgat
     8521 accctgaaaa aactcagcga tcgcctgaaa atgctggtct tcggccagga taaagccatt
     8581 gaggcgctga ctgaagccat taagatggcg cgtgcaggtt taggtcacga acataaaccg
     8641 gtcggttcgt tcctgtttgc cggtcctacc ggggtcggga aaacagaggt gacggtacag
     8701 ctttcgaaag cgttgggcat tgagcttctg cgctttgata tgtccgagta tatggaacgc
     8761 cataccgtca gccgtctgat tggtgcgcct ccgggatacg ttggttttga tcagggcggt
     8821 ttgctgaccg atgcggtaat caagcatcca catgcggtgc tgctgctgga cgaaatcgag
     8881 aaagcgcatc cggacgtgtt caatattctg ttgcaggtga tggacaacgg tacgctgacc
     8941 gataacaacg gacgcaaagc ggacttccgt aacgtggtgc tggtgatgac cactaacgcc
     9001 ggggtacggg aaactgagcg caaatccatt ggtcttatcc accaggataa cagcaccgat
     9061 gcgatggaag agatcaagaa gatctttaca ccggaattcc gtaaccgtct cgacaacatt
     9121 atctggttcg atcatctgtc aactgacgtg atccatcagg tggtggataa attcatcgtc
     9181 gagttgcagg ttcagctgga tcagaaaggt gtttctctgg aagtgagcca ggaagcgcgt
     9241 aactggctgg ccgagaaagg ttacgaccgg gcaatgggcg cacgtccgat ggcgcgtgtc
     9301 atccaggaca acctgaaaaa accgctcgcc aacgaactgc tgtttggttc gctggtggac
     9361 ggcggtcagg tgacggttgc gctggataaa gagaaaaatg agctgactta cggattccag
     9421 agtgcacaaa agcacaaggc ggaagcagcg cattaatctg attgtcaggt aggttggtga
     9481 agtccgtaat ctcgtcaggg gttacggact ttttatttat ggggtggaac ggtgaaaacc
     9541 ctatttttgg aggtgaaggt aagttgttga taattagtgc tgctggaagg taaggataaa
     9601 aaagggtgct gcaggagaat gggatggttt tgctttatta acaacgggct aaacgtgtag
     9661 tatttgagtt cactgccgta caggcagctt agaaatcgac ggggtgcggt aaaacctttg
     9721 cgaacgcgtt cactgccgta caggcagctt agaaattcac aggtaacata ctccacccgc
     9781 ccaccatgtt cactgccgta cagacagata aaatgcgaaa aaaaagctcg cactttcgta
     9841 cgagctcttc tttaaatatg gcggtgaggg ggggattcga acccccgata cgttgccgta
     9901 tacacacttt ccaggcgtgc tccttcagcc actcggacac ctcaccaaat tgttttgcta
     9961 ccaaacctca tgggtggcaa cggggcgcta ctatagggag ttggagtaaa acggtcaaga
    10021 agaattttaa tgataattat tgtttgctca tactgtaaac aacttgtgca gtatatctac
    10081 atcgagacaa gttatggact tatacttcca aagtacttca tacatatcac aaaataaaaa
    10141 ggccggttaa accgaccttt tactcgttct ttctcttcgc ccatcaggcg gtaaaacaat
    10201 cagcgactac ggaagacaat gcggcctttg ctcaggtcgt acggggtcag ttcaacagtc
    10261 actttgtcgc ccgtcaggat gcggatgtag tttttgcgca ttttaccgga gatgtgtgca
    10321 gtaaccacgt gaccgttttc taactctacg cggaacatgg tattaggcaa cgtttcaaga
    10381 acggtacctt gcatttcaat attgtcttct ttggccatct aatcctctgg ggtatcacta
    10441 ccgtaatttg aaccggcaag ataatgccga agttctgtaa ataagtaaag atttgcgcgc
    10501 taaatcgcaa caaacaggtt cggcacatta ctccgaaaac acacggctaa gccgcaccaa
    10561 aagcgcaacg tataagggag cggtgagata aacgatgggc gttacctgac gcgaaaaatt
    10621 ccttatcggc agcggggtaa tgagcgtaac caactctgcg accgcaatta taacactctg
    10681 gggagaaatg tgccgaaaac attcattctt gtggtgaaaa caagcaccgt ggtacccaga
    10741 aattattcgg caatcgtccg aggcgcattt gattgagata attaaggtaa tcccggcggg
    10801 gaatttcgca ggcaccaagc gatgctgtgt gatcgttaag gacctggcag tcgataagct
    10861 taccgccatg accgataaat tcctcacaga ataccagaag cgccgtttta gacgcatttt
    10921 ccatccggct gaacatggac tcgccacaaa atagcgttcc ctgggccacg ccgtacatac
    10981 cgccgacaag ctcatcttca cgccagactt caatggagtg ggcatgaccg agttcgtgaa
    11041 ggcgatggta ggcttcgacc acgccacgcg tgatccaggt tccttcttcg cgatcgctgg
    11101 cacagccttc aatgacctga ccaaaagcgt aattcatcgt gacacgatag ggcgagcgtt
    11161 tatgaaatcg cttcatacta cggctgatat gcagtgattc tggccatagc accgcgcggg
    11221 gatcgggcga ccaccagagg atggggtcgc ctggagaaaa ccacggaaaa ataccacgct
    11281 ggtaagccat taacaggcgc gcagggctaa gatcgccccc aagtgccagc aggccgttag
    11341 gctcacgtaa tgcgccttcc ggggaaggga aggctattga atggcgagaa agctgaacca
    11401 agcgcatgac cgcaaaactc cacgcaagtc ggatcgttca ataatagctt acaaaccctg
    11461 cttgaactgg taataacgcc cctgtctggc aagcagttct gcgtgagtac cttgctcaat
    11521 aatttgcccg ttgtccatca ctattatttg ttggaaacga gagagtccgc gaagtcgatg
    11581 ggtgaccatt aacaccgttt tctcacgcat catttttgca agcaattcaa ggatctggct
    11641 ttcggttgtg gcatctaagc cttcggtagg ttcatccagc aacaccagtg gcgcatcatg
    11701 taacagcgta cgggcgatag ccagacggcg cagttcacca ccggagagct ggcgtccgcc
    11761 ttcacctaac caactgttga gacctgcatc ctcgagcagc ttctccagac caacgcgacg
    11821 caagatctcc gacagagcct catcactact gccaggcgag gcgagtaaca gattatcacg
    11881 cagtgtggcg ctaaacagat gcactcgctg aggaacaacg ctgatggtct gtcgtagagc
    11941 cgcttcattc aggctggcta tggggctatc gttaagcaaa atctcgccct gttgcgggtc
    12001 ccatgcgcgg gtcagcagtt gtaacagtgt tgatttgccg catccggttc gcccgagaat
    12061 cgctatatgt tccccggcgt ttacctgaag agaaatccct ttaagtgcct gttgggattg
    12121 ctccggataa gtgaactgaa catcccgtaa tgtcagcgaa acgcgatcgg caacacgagt
    12181 ttgggtatca gggaaggtga cctccggttt ttgatccgtt aagtcagaga tacgtacggc
    12241 agaggcaatg acttgcccca gatgctgaaa tgcacccgtt actggtgcca gtgcttcaaa
    12301 cgcggctaac gcacaaaaga caaacagggc aattaatgcg ccgggttgag cattgccgcc
    12361 aacgccgcca gacgccatcc acagcatcag gatcaccgct aacgcgccaa tgagcagcat
    12421 tatcgcttgc gacaatgcgg tcagttcaga ttgacggcgt tgcgcttcca gccattgaat
    12481 ttctgtattc tctagttgcg tgcgataacg atcgctggca ccaaaaatgg tcagctcagc
    12541 ttgcccttgc agccaggccg tcagttgttg gcgatactgt ccgcgaagat gagtcagatt
    12601 ttgcccggtg ctttttcccg cacgataaaa cagcggtggc atcaggaaaa gcgtcagtaa
    12661 cataacgctg cccagcgtaa aggcgagggt gaaatcaagg aaacttaacc cgattgtcac
    12721 caccataatc accacaaaag cgcccaccag cggcgagata acgcgcaggt aaagatgatc
    12781 gagcgtatca acatccgcca ccacgcggtt gagcaattcg ccctgacgat agcgcgccag
    12841 tccggcaggg gagaggggca acaatttgct gaaggtgtaa atgcgcagat gctgcaacac
    12901 gcggaaagtc gcgtcgtgac ttaccagacg ttcaaaataa cgcccggcag tacgggtgat
    12961 tgcggcacca cgcacgcccg cagcgggtag catatagttg aagctgtaca gtccggcaac
    13021 ccccgcaacc gctgaggccg agaggaacca gccggaaagt gtcaacagac cgatactggc
    13081 gagcagcgtc acaattgcca gcacaatacc aagacttaac atccatttat gacgtttata
    13141 cagtgccaga tagggtagca aagcgcgcat ttaaatctcc tcctgacgat gggccagtaa
    13201 tgtggcgaat gggccaccag ccacacttaa ttccgcgtaa cgtccttgct caataatccg
    13261 gccatcctgc ataacccaaa tgacatccca gtcagcaaga tcttctaact ggtgggtgac
    13321 cattaacgtt gtctggcgca gagaggcggc attcagcgcc tccattacgc gctgttcact
    13381 gtgagcatca aggctggcag cgggttcatc caacagtaat agcgaacagg gatttagtaa
    13441 cgcacgggcc accgccacgc gctgcgcctg ccccacggaa aggcgggcag cctggtcgcc
    13501 aacaggtgta tcaacgcctt gtggcaggag cggtagaaac tcgctgaccc aggcgttatc
    13561 cagcgctgct tgtaattctt gttcgctggc atcaggtcgc gccagtagta cgttatcccg
    13621 caattttgct gccggtaatt gtgggttttg cccaacccag gagagatgtt tacgccatga
    13681 ttctggtgat aaatcacgta attctatccc gttgattcgt aatgatccct gatatgagag
    13741 aaaaccagaa agcgcgttca gcagtgagct tttacctgaa ccgctgcggc caaccaacac
    13801 tgcacgttgg cctgctggca aagtaaagtt cagcggtccg gccagcgttt taccttccgg
    13861 cgacgtgata aacagatcct cggcctcaat ggtcagcgga tcggtcaatg ctaattccgc
    13921 ctcaccgcgc tgcggatggg cgagcggggt ttccataaac gttttcagac tgtcagctgc
    13981 accaacagcc tgggctttag catgataaaa cgtaccgaga tcgcgtaatg gctggaaaaa
    14041 ctctggcgca aggatcaggg ccagaaaacc tgcagccagc gtcaccccag tatcgtagtg
    14101 accaaaatcc agctcgccga gataggaaaa accaaagtag accgccacca gagcaattga
    14161 cagcgaggta aaaaattcga gaatgccgga ggataaaaac gccagtcgta gcacttccat
    14221 tgtccgttgg cggaaatctt ccgaagcaga acgaatactt tcaatttcag cttcaccacg
    14281 accaaaaata cgcaatgttt ccatgccgcg caggcgatcg aggaaatgcc cacttaagcg
    14341 agcaagagcg agaaagttac gccggttagc atcggcagcc cccattccaa ccagcgccat
    14401 aaacaacgga attagcggtg ctgtgcccag cagaatgagc gccgcagccc agttagaggg
    14461 gaagatcgcc accacaatca gcaacggcac cgacactgcc agcgccattt gcggcagata
    14521 gcgtgcatag taatcatgca tatcgtcaat ttgctcgagt accagcgtcg cccagctccc
    14581 cgcaggttta ccctgaatcc acgctggccc tgcttgttgc agacggtcga gaacctgacg
    14641 gcggatggca aagcggatat gctgcccggc gtgataaccc acccgttcgc gtaaccagac
    14701 cacccatgcg cgcaatacaa aggtcagaac cagtaacgta aagggaagca gcagggcttc
    14761 acggggaata ttctccataa tcatatgttg cagaattcgc gccatgaacc aggcctgggc
    14821 aatgatcaat atgccgctta caaagcccag cagacgagaa atattcagcc aacgttggga
    14881 gatgacgctt tgctgtttta accagcgggt taactctttt tgacgagatt tattcattgc
    14941 acgcttagca ggtgagttat cagaattatt tgcagagcaa tgttacaacg gggaaaaaat
    15001 aaaggcgacc catagtcgca aggtgtcgcc ttctttactt ttgttactga tttgtaaaat
    15061 tattttgcgt cagctaaacc atcgaggtag cgttccgcat caagtgctgc catgcagcca
    15121 gtaccggccg aagtaatggc ctggcgataa atgtgatcca tcacgtcgcc tgcggcaaag
    15181 acgccaggaa tgctggtctg ggtggcatta ccatgaatac ccgactgtac tttgatgtag
    15241 ccgttttcca gttccagctg cccttcgaaa atcgcagtat tcgggctgtg accgatagca
    15301 acaaacagac cggcaacgtc gagtgactcg atgttatcgc tgttttgcgt atcgcgcaga
    15361 cgaacgccag tgacacccat ttgatcgccg gtcacttctt ccagcgtacg gttggtgtgc
    15421 agaatgatgt tgccgttctc cactttatcc atcagacgct taataaggat tttttccgcg
    15481 cggaaaccgt cacggcggtg aatcagatgc acttccgaag cgatgttaga cagatacagc
    15541 gcctcttcaa ccgcggtatt gccgccgccg atgaccgcaa ctttctggtt gcgatagaag
    15601 aaaccgtcgc aggttgcaca agcagaaacc ccacggcctt taaaggcttc ttcagagggc
    15661 aggccgagat agcgtgcaga agctccggtg gcaataatca gcgcgtcgca agtgtattcg
    15721 ccgttatcgc cattcagacg gaacggacgg ttttgcagat ccaccttgtt gatatgatca
    15781 aaaatgatct cagtttcaaa cttggtggca tgttcgtgca tgcgcgccat taataacgga
    15841 ccggtcagat cgtttggatc accaggccag ttttccactt ccgtggtggt ggtcagttgg
    15901 ccgccttttt ccatgccggt aatcagcaca ggttgcaggt tggcgcgcgc cgcgtagaca
    15961 gcagcggtgt atcccgccgg gcctgaaccc aggataagca gtttactgtg tttggtcgtg
    16021 cccatgagat ccccatagtt gttggcagac aatgggcagg attgtaggga atttacagac
    16081 gtaaaaaaag agtatgacga ttttgttaac aatttgtgca atcggcagca tcgataagca
    16141 ggtcaaattc tcccgtcatt atcacctctg ctacttaaat ttcccgcttt ataagccgat
    16201 taaatgatga ataaacgccc ctgttaatga atatctggca tgttgtacta aaaatcgatg
    16261 ttttgctttg acaatcccct ggtgttttgc gaaaacattc gaggaagaaa aaaacagtat
    16321 tcttatatgc gcataaccat gtatgtaaat accatgttta ccgtgctagt gaaatctacg
    16381 tatggcgtgg acagacgcca ttcgtgatgt cgatagctgc cacaaggcaa cggtcttctc
    16441 accgtagacc caggcattgc gcgccgtgaa tcttcatgat ttcggtctat cgtgacgggt
    16501 agcgactctg aacagtgatg tttcagggtc agacaggagt agggaaggaa tacagagaga
    16561 caataataat ggtagatagc aagaagcgcc ctggcaaaga tctcgaccgt atcgatcgta
    16621 acattcttaa tgagttgcaa aaggatgggc gtatttctaa cgtcgagctt tctaaacgtg
    16681 tgggactttc cccaacgccg tgccttgagc gtgtgcgtcg gctggaaaga caagggttta
    16741 ttcagggcta tacggcgctg cttaaccccc attatctgga tgcatcactt ctggtattcg
    16801 ttgagattac tctgaatcgt ggcgcaccgg atgtgtttga acaattcaat accgctgtac
    16861 aaaaacttga agaaattcag gagtgtcatt tagtatctgg tgatttcgac tacctgttga
    16921 aaacacgcgt gccggatatg tcagcttacc gtaagttgct gggggaaacc ctgctgcgtc
    16981 tgcctggcgt caatgacaca cggacatacg ttgttatgga agaagtcaag cagagtaatc
    17041 gtctggttat taagacgcgc taacacggaa caggtgcaaa atcggcgtat tttgattaca
    17101 ctcctgttaa tccatacagc aacagtactg gggtaacctg gtactgttgt ccgttttagc
    17161 atcgggcagg aaaagcctgt aacctggaga gcctttcttg agccaggaat acactgaaga
    17221 caaagaagtc acattgacaa agttaagtag cggccgccgc cttctggagg cattgctgat
    17281 ccttattgtc ctgtttgccg tctggttgat ggctgcctta ctaagcttta acccttcgga
    17341 ccccagctgg tcgcaaacgg cctggcatga acctatccat aatttaggtg ggatgcccgg
    17401 tgcgtggttg gcagatacgc tgttctttat ttttggcgtg atggcttaca ccattcccgt
    17461 cattattgtc ggcggttgtt ggtttgcctg gcgtcatcag tccagcgacg aatacattga
    17521 ttattttgcc gtttcgctac gcatcattgg cgttttggcg ctcatcctta cctcctgtgg
    17581 tctggcggca atcaacgctg acgatatctg gtattttgcc tccggtggcg tcattggcag
    17641 cttactaagc actacgctac aaccactgct acacagtagc gggggaacta ttgcgctgct
    17701 ctgcgtttgg gcagcgggcc tgacgttgtt caccggttgg tcatgggtga ccattgctga
    17761 aaaactcggc ggctggattt taaacattct caccttcgcc agtaatcgta cccgtcgcga
    17821 tgatacctgg gtcgatgaag atgagtatga agacgacgaa gagtatgaag atgaaaatca
    17881 cggcaaacag catgaatcac gccgtgcccg tattcttcgc ggcgcgctag cgcgtcgtaa
    17941 acggttggcg gaaaaattca ttaatccgat ggggcggcaa acagacgctg cgttgttctc
    18001 cggtaagcgg atggatgatg acgaagagat tacctacact gcacgcggtg tggctgccga
    18061 cccggacgac gtcctatttt cgggcaatcg tgcaacgcag ccagaatatg acgaatacga
    18121 tccattatta aacagtgcgc caattaccga acctgtcgct gtagcagctg ctgctaccac
    18181 ggcgacacaa agctgggctg cgccggttga acctgtgact cagacgccgc ctgttgcctc
    18241 tgttgatgtt ccaccttcgc aacctacagt agcctggcag cctgtaccgg gtccacaaac
    18301 gggagagccg gttattgctc ctgcaccgga aggttaccca cagcagtcac aatatgcgca
    18361 gcctgcagtg caatataatg agccgctgca acaaccagta cagccgcagc agccgtatta
    18421 tgcacctgca gctgaacaac ctgcgcaaca gccgtattat gcccctgcgc cagaacaacc
    18481 ggtggcaggt aacgcctggc aagccgaaga gcagcaatcc acttttgctc cacagtctac
    18541 ataccagact gagcaaactt atcagcagcc agccgctcag gagccgttgt accaacagcc
    18601 gcaacccgtt gaacagcagc ctgttgtgga gcctgaaccc gttgtagaag agacaaaacc
    18661 cgcgcgtccg ccgctttact attttgaaga agtggaagag aagcgagccc gtgaacgtga
    18721 acaacttgcg gcctggtatc aaccgattcc agaaccggtt aaagaaccag aaccgatcaa
    18781 atcttcgctg aaagcacctt ctgttgcagc agtacctcca gtagaagccg ctgccgctgt
    18841 ttctccgctg gcatctggcg tgaaaaaagc gacactggcg acgggggctg ccgcaaccgt
    18901 tgccgcgcca gtcttcagtc tggcaaatag cggtggaccg cgtccgcagg tcaaagaggg
    18961 gattggcccg cagttgccac gaccgaaacg tatccgcgtg ccaactcgtc gtgaattggc
    19021 gtcttacggt attaagctgc cctcacagcg tgcggcggaa gaaaaagccc gtgaagccca
    19081 gcgcaatcag tatgattctg gcgatcagta taacgatgat gaaatcgatg cgatgcagca
    19141 ggatgaactg gcacgtcagt tcgcccagac acagcagcaa cgctatggcg aacagtatca
    19201 acatgatgtg cccgtaaacg cagaagatgc agatgctgcg gcagaggctg aactggctcg
    19261 tcagtttgcg caaactcaac aacaacgtta ttccggcgaa caaccggctg gggcgaatcc
    19321 gttctcgctg gatgattttg aattttcgcc aatgaaagcg ttgctggatg atggtccaca
    19381 cgaaccgctg tttacgccaa ttgttgaacc tgtacagcag ccgcaacaac cggttgcacc
    19441 acagcagcag tatcagcagc cacaacagcc ggttgcgcca cagcagcagt atcagcagcc
    19501 acaacagcag gttgcgccgc agccacaata tcagcagccg caacaaccgg ttgcgccaca
    19561 gcagcaatat cagcagccgc aacaaccagt tgcgccgcag ccgcagtatc aacagccgca
    19621 acaaccggtt gcaccgcagc cgcagtatca gcagccacaa cagccagttg cgccacagcc
    19681 gcaggatacc ctgcttcatc cgctgttgat gcgtaatggc gacagccgtc cgttgcacaa
    19741 accgacgacg ccactgcctt ctctggattt gctgacaccg ccgccgagcg aagtggagcc
    19801 ggtagatacc tttgcgcttg aacaaatggc gcgtctggtg gaagcgcgtc tggctgattt
    19861 ccgtattaaa gccgatgtcg ttaattactc tccggggcca gttatcactc gctttgaact
    19921 taacctggct cctggcgtaa aagcggcgcg catttctaac ttgtcacggg accttgcccg
    19981 ttcactttcg acggtggcgg tgcgtgtcgt tgaagttatt cctggcaaac cctatgtagg
    20041 tctggagtta ccaaataaaa aacgacaaac cgtttatctg cgcgaagttt tggataacgc
    20101 caaattccgc gataatccgt cgccattaac cgtggtgctg ggtaaagata tcgccggtga
    20161 gccggtggtt gccgatctgg cgaaaatgcc gcacttgttg gttgcgggga ctaccggttc
    20221 cggtaaatct gtcggtgtga acgcgatgat cctgagcatg ctttataaag cacagccaga
    20281 agatgtgcgt ttcatcatga tcgacccgaa aatgctggag ctttcggttt atgaaggcat
    20341 tccgcacctg ttaacggaag tcgttactga tatgaaagat gccgccaacg cgctgcgctg
    20401 gtgtgttaac gagatggagc gtcggtataa actgatgtct gcgctgggtg tgcgtaatct
    20461 ggcgggttat aacgaaaaaa ttgctgaagc cgatcgcatg atgcgtccga ttccagaccc
    20521 gtactggaag ccgggtgaca gtatggatgc ccagcatccg gtgctgaaaa aagaaccata
    20581 cattgtcgtg ttggttgacg aatttgccga cctgatgatg acggtaggta aaaaagtgga
    20641 agagctgata gctcgtctgg cgcaaaaagc ccgtgccgcg ggtatccacc tcgtactggc
    20701 aactcagcgt ccatcggttg atgttattac tggtctgatt aaagcgaata ttccgacccg
    20761 tatcgccttt accgtatcca gtaagattga ctcacgtacc attcttgatc aggctggcgc
    20821 ggaatcactg ctgggtatgg gggatatgct ctactctggg ccgaactcca cgttgccggt
    20881 acgtgtccat ggtgcttttg ttcgcgatca ggaagttcat gccgtggtgc aggactggaa
    20941 agcgcgtggt cgcccacagt atgttgatgg catcacctcc gacagcgaaa gcgaaggtgg
    21001 tgcgggtggt ttcgatggcg ctgaagaact ggatccgttg ttcgatcagg cggtgcagtt
    21061 tgtcactgaa aaacgcaaag cgtcaatttc tggcgtacag cgtcagttcc gcattggtta
    21121 taaccgtgca gcgcgtatta tcgaacagat ggaagcgcag gggattgtca gcgaacaggg
    21181 gcacaacggt aatcgtgaag tgctggcccc accgccgttt gactaattaa tgcattgccg
    21241 gataaggcgc ggtagcgtcg catccggcac tctatcaact gaaaattcag tattttcttc
    21301 tttcctcaag ctgattatta gcctggaata gagagtagag ggaactcccg atcgggagtg
    21361 acgtaatttg aggaataatg atgaaaaaaa ttgctatcac ctgtgcatta ctctcaagct
    21421 tagtagcaag cagcgtttgg gctgatgccg caagcgatct gaaaagccgc ctggataaag
    21481 tcagcagctt ccacgccagc ttcacacaaa aagtgactga cggtagcggc gcggcggtgc
    21541 aggaaggtca gggcgatctg tgggtgaaac gtccaaactt attcaactgg catatgacac
    21601 aacctgatga aagcattctg gtttctgacg gtaaaacact gtggttctat aacccgttcg
    21661 ttgagcaagc tacggcaacc tggctgaaag atgccaccgg taatacgccg tttatgctga
    21721 ttgcccgcaa ccagtccagc gactggcagc agtacaatat caaacagaat ggcgatgact
    21781 ttgtcctgac gccgaaagcc agcaatggca atctgaagca gttcaccatt aacgtgggac
    21841 gtgatggcac aatccatcag tttagcgcgg tggagcagga cgatcagcgc agcagttatc
    21901 agctgaaatc ccagcaaaat ggggctgtgg atgcagcgaa atttaccttc accccgccgc
    21961 aaggcgtcac ggtagatgat caacgtaagt agaggcacct gagtgagcaa tctgtcgctc
    22021 gatttttcgg ataatacttt tcaacctctg gccgcgcgta tgcggccaga aaatttagca
    22081 cagtatatcg gtcagcaaca tttgctggct gcggggaagc cgttgccgcg cgctatcgaa
    22141 gccgggcatt tgcattctat gatcctctgg gggccaccgg gtaccggcaa aacaaccctc
    22201 gctgaagtga ttgcccgcta tgcgaacgct gatgtggaac gtatttctgc cgtcacctct
    22261 ggcgtgaaag agattcgcga ggcgatcgag cgcgcccggc aaaaccgcaa tgcaggtcgc
    22321 cgcactattc tttttgttga cgaagttcac cgtttcaaca aaagccagca ggatgcattt
    22381 ctgccacata ttgaagacgg caccatcact tttattggcg caaccactga aaacccgtcg
    22441 tttgagctta attcggcact gctttcccgt gcccgtgttt atctgttgaa atccctgagt
    22501 acagaggata ttgagcaagt actaactcag gcgatggaag acaaaacccg tggctatggt
    22561 ggtcaggata ttgttctgcc agatgataca cgacgtgcca ttgcagaact ggtgaatggc
    22621 gacgcgcgcc gggcgttaaa tacgctggaa atgatggcgg atatggccga agtctatgat
    22681 agcggtaagc gggtcctgaa gcctgaatta ctgaccgaaa tcgccggtga acgtagcgcc
    22741 cgctttgata acaaaggcga tcgcttttac gatctaattt ccgcactgca taagtcggta
    22801 cgtggtagcg cacccgatgc ggcgctgtac tggtatgcgc gaattattac cgctggtggc
    22861 gatccgttat atgtcgcgcg tcgctgtctg gcgattgcgt ctgaagacgt cggtaatgcc
    22921 gatccacggg cgatgcaggt ggcaattgcg gcctgggatt gctttactcg cgttggcccg
    22981 gcggaaggtg aacgcgccat tgctcaggcg attgtttacc tggcctgcgc gccaaaaagc
    23041 aacgctgtct acactgcgtt taaagccgcg ctggccgatg ctcgcgaacg cccggattat
    23101 gacgtgccgg ttcatttgcg taatgcgccg acgaaattaa tgaaggaaat gggctacggg
    23161 caggaatatc gttatgctca tgatgaagca aacgcttatg ctgccggtga ggtttacttc
    23221 ccgccggaaa tagcacaaac acgctattat ttcccgacaa acaggggcct tgaaggcaag
    23281 attggtgaaa agctcgcctg gctggctgaa caggatcaaa atagccccat aaaacgctac
    23341 cgttaatgtt atcgttgcgg taatgttgtt actgtatccc tgtggtcgca ggctgtggcc
    23401 acatctccca tttaattcga taagcacagg ataagcatgc tcgatcccaa tctgctgcgt
    23461 aatgagccag acgcagtcgc tgaaaaactg gcacgccggg gctttaagct ggatgtagat
    23521 aagctgggcg ctcttgaaga gcgtcgtaaa gtattgcagg tcaaaacgga aaacctgcaa
    23581 gcggagcgta actcccgatc gaaatccatt ggccaggcga aagcgcgcgg ggaagatatc
    23641 gagcctttac gtctggaagt gaacaaactg ggcgaagagc tggatgcagc aaaagccgag
    23701 ctggatgctt tacaggctga aattcgcgat atcgcgctga ccatccctaa cctgcctgca
    23761 gatgaagtgc cggtaggtaa agacgaaaat gacaacgttg aagtcagccg ctggggtacc
    23821 ccgcgtgagt ttgactttga agttcgtgac cacgtgacgc tgggtgaaat gcactctggc
    23881 ctcgactttg cagctgcagt taagctgact ggttcccgct ttgtggtaat gaaagggcag
    23941 attgctcgca tgcaccgcgc actgtcgcag tttatgctgg atctgcatac cgaacagcat
    24001 ggctacagtg agaactatgt tccgtacctg gttaaccagg acacgctgta cggtacgggt
    24061 cagctgccga aatttgctgg cgatctgttc catactcgtc cgctggaaga agaagcagac
    24121 accagtaact atgcgctgat cccaacggca gaagttccgc tgactaacct ggtgcgcggt
    24181 gaaatcatcg atgaagatga tctgccaatt aagatgaccg cccacacccc atgcttccgt
    24241 tctgaagccg gttcatatgg tcgtgacacc cgtggtctga tccgtatgca ccagttcgac
    24301 aaagttgaaa tggtgcagat cgtgcgccca gaagactcaa tggcggcgct ggaagagatg
    24361 actggtcatg cagaaaaagt cctgcagttg ctgggcctgc cgtaccgtaa aatcatcctt
    24421 tgcactggcg acatgggctt tggcgcttgc aaaacttacg acctggaagt atggatcccg
    24481 gcacagaaca cctaccgtga gatctcttcc tgctccaacg tttgggattt ccaggcacgt
    24541 cgtatgcagg cacgttgccg cagcaagtcg gacaagaaaa cccgtctggt tcataccctg
    24601 aacggttctg gtctggctgt tggtcgtacg ctggttgcag taatggaaaa ctatcagcag
    24661 gctgatggtc gtattgaagt accagaagtt ctgcgtccgt atatgaacgg actggaatat
    24721 attggctaat acccaatttt tctgaatcta aaaagcgcct ttagggcgct ttttttgtct
    24781 ccctttgata ccgaacaata attactcctc acttacacgt aatactactt tcgagtgaaa
    24841 atctacctat ctctttgatt ttcaaattat tctatgtata caagcctata tagcgagctg
    24901 ctatagaaat aattacacaa tacggtttgt tactggaatc aatcgtgatc aagcttgagt
    24961 gagccattat gaaaacgaaa atccctgatg cggtattggc tgctgaggag agtcgccgtg
    25021 gtttggtaaa aacgacagcg atcggcggcc tggcaatggc cagcagcgca ttaacattac
    25081 cttttagtcg gattgcgcac gctgtcgata gcgccattcc aacaaaatca gacgaaaagg
    25141 ttatctggag cgcctgtaca gttaactgtg gtagtcgctg cccgctacgt atgcacgtcg
    25201 tggacggtga aatcaaatat gtcgaaacga caataccggc gatgacaatt acgacggcct
    25261 acactaggtt cgcgcctgcc tgcgtgggcg ttccatgcgt caccgtgtct acaatccgga
    25321 ccgcctgaaa tatccgatga aacgagtcgg ggcgcgcggt gaaggcaaat tcgagcgcat
    25381 tagctgggaa gaagcctacg acatcatcgc gaccaatatg cagcgcctga tcaaagagta
    25441 cggcaacgag tctatctatc tgaactatgg caccggtacg ctgggcggca ccatgacccg
    25501 ctcctggccg ccgggaaata ccctggtcgc gcgcctgatg aactgctgcg gcggttatct
    25561 taaccattac ggtgactact cttccgcgca aattgcggaa ggtttgaacc atacctacgg
    25621 cggctgggca gatggtaaca gcccgtcgga tatcgaaaac agtaaattgg tagtgctgtt
    25681 tggtaataac ccaggcgaaa cgcgaatgag tggcggtggg gtgacttact atcttgaaca
    25741 ggcacgccag aaatctaatg ctcgcatgat catcatcgat ccgcgctata ccgacaccgg
    25801 tgccgggcgc gaagatgagt ggatccctat tcgtccagga acagatgccg cactggttaa
    25861 cggtctggcg tacgtcatga tcactaaaaa cctggtggat caggcattcc tcgataaata
    25921 ttgcgttggc tatgacgaga aaaccctgcc cgccagtgcg ccgaaaaatg gtcattataa
    25981 agcttatatt ctgggtgaag ggccagatgg cgtggctaaa acaccggaat gggcctcgca
    26041 aatcaccggt gttccggcag acaaaatcat caaactggct cgtgaaatcg gcagtaccaa
    26101 accggtgttt atcagccagg gatggggccc gcagcgtcac gctaacggtg aaatcgcaac
    26161 ccgtgctatc tcgatgctgg cgattctgac cggtaacgtt ggtattaatg gagggaacag
    26221 cggcgcgcgt gaaggttcat acagcttacc gtttgtccgt atgccgacct tggaaaaccc
    26281 gatccagacc agcatttcga tgtttatgtg gaccgatgcc attgaacgtg gcccggaaat
    26341 gacggcgctg cgtgatggtg tgcgcgggaa agataagctg gatgtgccga tcaaaatgat
    26401 ctggaactat gccggtaact gcctgattaa ccagcattct gaaatcaacc gtacccatga
    26461 aatcctccag gatgataaga agtgcgagct gattgtggtt atcgactgcc acatgacctc
    26521 atcggcgaaa tatgctgaca tcctgctgcc tgactgcacc gcttccgaac agatggactt
    26581 tgcactggat gcatcctgcg ggaatatgtc ttacgtgatt ttcaacgatc aggtgattaa
    26641 accgcgcttt gaatgtaaga ccatctatga aatgaccagc gaactggcaa aacgtcttgg
    26701 cgttgagcaa cagtttactg aaggccgtac ccaggaagag tggatgcggc atctgtatgc
    26761 ccagtcgcgg gaagcgattc ctgaactgcc aacgtttgaa gagttccgca agcaggggat
    26821 ctttaaaaag cgcgacccac aagggcatca cgttgcttat aaagccttcc gtgaagatcc
    26881 gcaggcaaac ccactgacta cgccatcggg caaaattgag atttattcgc aggcgctggc
    26941 tgacattgcc gctacctggg aattgccaga aggcgatgtg atcgatccac tgccgatcta
    27001 cacgccgggc tttgaaagtt atcaggatcc gctgaacaaa cagtatccgc tgcagcttac
    27061 aggtttccac tataaatctc gcgttcactc aacttacggc aacgttgatg tgctgaaagc
    27121 ggcttgccgt caggaaatgt ggatcaaccc gcttgatgcc caaaaacgcg gtatccacaa
    27181 cggcgataaa gtcaggatct ttaacgatcg tggtgaggtt catattgagg cgaaagtgac
    27241 gccacgaatg atgccgggcg tggtcgcact gggtgaaggc gcctggtatg acccggatgc
    27301 aaaacgtgtc gataagggcg gttgtatcaa cgtactgacc actcaacgtc cgtctcctct
    27361 cgctaagggg aatccgtcac atacaaacct tgttcaggtt gaaaaggtgt aaggagtaac
    27421 cgatgacaac ccagtatgga ttttttattg attccagccg ttgcaccggt tgcaaaacct
    27481 gcgagctggc ttgtaaagac tacaaagatt tgacgccaga agtcagtttc cgccgcattt
    27541 atgaatatgc tggcggcgac tggcaggaag ataacggtgt ctggcaccag aacgtgtttg
    27601 cctactatct gtcgatttcg tgcaaccact gcgaagatcc ggcttgtact aaagtctgcc
    27661 cgagcggtgc gatgcataaa cgcgaagatg gttttgttgt ggtcgatgaa gatgtatgca
    27721 ttggctgccg ttactgtcat atggcttgcc cgtatggcgc accgcaatat aacgaaacga
    27781 aaggccatat gaccaaatgc gatggttgtt atgaccgtgt tgcggagggt aaaaagccga
    27841 tctgtgttga atcttgcccg ctgcgggcgc tggatttcgg ccctatcgac gagctgcgta
    27901 aaaaacatgg cgatctggcg gccgttgcgc cgttgccgcg agctcacttt accaaaccga
    27961 atattgtgat caaacccaat gccaatagcc gcccgaccgg ggataccact ggctatctgg
    28021 caaacccgaa ggaggtgtga gatgggaagt ggatggcatg aatggccgct gatgatcttc
    28081 acggtcttcg ggcaatgtgt ggcgggtggt tttatcgttc tggctttggc gctgctcaaa
    28141 ggcgacctgc gagcagaagc ccagcagcgt gttatcgcct gcatgtttgg cttatgggta
    28201 ctgatgggca ttggctttat cgcctctatg ctccatcttg gttcaccgat gcgcgccttt
    28261 aactcgctca accgggtagg ggcttcagca ctcagtaacg aaatcgccag cggttcgatc
    28321 ttttttgctg taggcggcat cggctggttg ctggcaatgc tgaaaaagct gtcaccggca
    28381 ttgcgtacgc tatggctggt ggtgacgatg gttcttggcg tcgtctttgt ctggatgatg
    28441 gtgcgtgtgt ataacagcat tgataccgtt ccgacctggt acagcatctg gacgccgatg
    28501 ggctgggcga tgcgtctgct gccagccatt tctgtactgg cactggtggt gagtggcgtg
    28561 atgtcagtga tgcagggcac agagctggca actattcata gttctgtgca gcaggccgca
    28621 gcgctggtgc cggactatgg tgcgctgatg tcctggcgga tcgtgctttt ggccgttgcc
    28681 ctgtgcttgt ggattgcacc acagctaaaa ggttatcagc ctgcggttcc gctactatcc
    28741 gtctcattca ttctgcttct ggcaggggaa ttaatcggtc gcggcgtatt ctatggtttg
    28801 catatgaccg tggggatggc cgtcgcaagc taatcagtaa caaccggggt ttcggccccg
    28861 gttctctttt atttctgctt cgttaacgtg tcataactgg tcatcaaatt acgataatca
    28921 ggaatatggt tagagaacaa cgtcgccagt ccttcaatat cattacgcca gtcacggtgc
    28981 agctcgcaag ccacgccgaa ccatgtcatc aattgcgcac cagcttgcga catgcgatcc
    29041 cacgccgaat gccgggtaat ttcattaaag gtccctgatg catcagtcac gacaaagaca
    29101 tcaaaccctt cttcaatggc tgaaagcgcc gggaatgcca cgcaaacttc ggttaccaca
    29161 ccggcaataa ttaactgttt tttacctgtc gctttgactg cttttacaaa atcttcgtta
    29221 tcccaggcgt taatattgcc cgggcgagca atgtaaggtg catcggggaa ttgtgctttc
    29281 agttctagaa ctaatgggcc gttaggaccg gtttcaaaac tggtggtaag aatagttggt
    29341 aagttgaaat acttagccag gtcacccagc gccagcacgt tatttttaaa cttatcgggt
    29401 tcgatatccc gtacaaggga aagtaaacca gcctggtgat caacaagcaa aacggcagca
    29461 tcatttttat caagacgaac atacggtttg gtcatcctct tctcctttcg agagtcagtg
    29521 gtacgtctga aatcggtcag acgcgatgca ttgctctgaa agcatagacg ggaaatatga
    29581 gtttgctgtg accatgaaat ttttcgactg aacgaagcgt atgaaatttg tgttagatca
    29641 ataaaaacaa tcagatgaga ttaatcatct cttcagcatt agcgttatga atgagtaata
    29701 taaatcgata ataccgccgc tgcgatatgc gcgtattttc acccattgac aatgtttttg
    29761 gcggtggcat gatgcgcatg aaatttgaac ttcctcacgg ttttaattca tgtccacgta
    29821 tacccggcct gtcatgcttt tgctgtctgg cctgcttttg ttgactctgg cgatcgcggt
    29881 gttaaataca ctcgtgccgc tttggctcgc ccaggaacac atgtccacat ggcaggtagg
    29941 cgttgtcagc tcatcctatt ttaccggcaa ccttgtcggt acattgctga cagggtatgt
    30001 cattaaacgc attggcttta accgcagcta ttatctgtcc tccttcattt ttgctgctgg
    30061 ctgtgccggc cttggcctga tgattggttt ctggagctgg ttggcgtggc gttttgtcgc
    30121 gggcgtcggc tgtgccatga tttgggtggt tgttgagagc gcgctgatgt gcagtgggac
    30181 gtcacgtaac cgtgggcgtt tgcttgctgc gtatatgatg gtttattacg tgggaacgtt
    30241 tttaggccag ttactggtca gcaaagtctc aaccgagctg atgagcgtat tgccgtgggt
    30301 tacaggtttg acattggcag ggatcttacc gctgttgttt acgcgtgtcc tgaatcagca
    30361 ggctgaaaac catgattcga cgtcaattac ggcaatgcta aaactccgtc aggcgcggct
    30421 tggcgtgaat ggctgcatta tctcaggaat cgttctggga tctctatatg gcctgatgcc
    30481 tctgtacctc aatcacaaag gggtgagcaa tgccagtatt ggtttctgga tggcggtact
    30541 ggtcagtgcg ggtatcctcg gacaatggcc gattggacgt ctggcggata agtttggtcg
    30601 actgctggtt ttgcgtgttc aggtctttgt cgtcattctc ggcagtattg cgatgcttag
    30661 ccaggcggcg atggccccag cgttattcat cctcggtgcc gctggcttta cgctatatcc
    30721 ggtggcgatg gcctgggctt gtgagaaagt tgaacatcat caactggtgg cgatgaacca
    30781 agccttactg ttgagctata ccgtgggaag tctgcttggc ccgtcattta gcgctatgct
    30841 aatgcagaat ttctccgata atttattgtt tatcatgatc gccagcgtat cgtttatcta
    30901 tttgctgatg ctgcttcgca acgccggtca tacgccgaaa cccgttgctc acgtgtaaat
    30961 gaattcaagc aaagtgtgaa cttactgttt cacactctgc ttttttgttt cttctatctg
    31021 acttgctttt ttccaaattt tattcgttta aaaataaaat gtgcagcagg ttataatttt
    31081 gcatttcgct atttccgcac ttcttatttg ccgcgcataa tccctcgttt tacagatgcc
    31141 cctttaattt tggcgaagga tttgtctatg gctgggaatg ttcagg
//
LOCUS       DAZPCS010000031        30770 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZPCS010000031 DAZPCS010000000
VERSION     DAZPCS010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30770
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.031"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..1150)
                     /gene="setB"
                     /locus_tag="ACHUQ7_001422"
     CDS             complement(<1..1150)
                     /gene="setB"
                     /locus_tag="ACHUQ7_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFR1134902.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIAT"
     gene            1518..2648
                     /gene="fruB"
                     /locus_tag="ACHUQ7_001423"
     CDS             1518..2648
                     /gene="fruB"
                     /locus_tag="ACHUQ7_001423"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFR1134903.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            2648..3586
                     /gene="fruK"
                     /locus_tag="ACHUQ7_001424"
     CDS             2648..3586
                     /gene="fruK"
                     /locus_tag="ACHUQ7_001424"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFR1134904.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            3603..5294
                     /gene="fruA"
                     /locus_tag="ACHUQ7_001425"
     CDS             3603..5294
                     /gene="fruA"
                     /locus_tag="ACHUQ7_001425"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFR1134905.1"
                     /translation="MKTLLIIDANLGQARAYMVKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            5718..6659
                     /locus_tag="ACHUQ7_001426"
     CDS             5718..6659
                     /locus_tag="ACHUQ7_001426"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFR1134906.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQNHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            6647..7585
                     /gene="psuG"
                     /locus_tag="ACHUQ7_001427"
     CDS             6647..7585
                     /gene="psuG"
                     /locus_tag="ACHUQ7_001427"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFR1134907.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTSGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            7679..8929
                     /locus_tag="ACHUQ7_001428"
     CDS             7679..8929
                     /locus_tag="ACHUQ7_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFR1134908.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRVNRNELFTAICSGMASIAGSVMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(9000..9694)
                     /locus_tag="ACHUQ7_001429"
                     /pseudo
     CDS             complement(9000..9694)
                     /locus_tag="ACHUQ7_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
     gene            9825..10766
                     /gene="rihB"
                     /locus_tag="ACHUQ7_001430"
     CDS             9825..10766
                     /gene="rihB"
                     /locus_tag="ACHUQ7_001430"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFR1134909.1"
                     /translation="MEKRKIILDCEPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERVGGPAGELFSD
                     IMNFTLKTQFEYYGLAGGPVHDATCIGYLINPDGIKTQDMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            10866..12116
                     /locus_tag="ACHUQ7_001431"
     CDS             10866..12116
                     /locus_tag="ACHUQ7_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFR1134910.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEADATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(12172..13260)
                     /locus_tag="ACHUQ7_001432"
     CDS             complement(12172..13260)
                     /locus_tag="ACHUQ7_001432"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFR1134911.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKDKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTVPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(13263..14120)
                     /gene="nfo"
                     /locus_tag="ACHUQ7_001433"
     CDS             complement(13263..14120)
                     /gene="nfo"
                     /locus_tag="ACHUQ7_001433"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFR1134912.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(14194..15243)
                     /locus_tag="ACHUQ7_001434"
     CDS             complement(14194..15243)
                     /locus_tag="ACHUQ7_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFR1134913.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            15342..16223
                     /gene="yieE"
                     /locus_tag="ACHUQ7_001435"
     CDS             15342..16223
                     /gene="yieE"
                     /locus_tag="ACHUQ7_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFR1134914.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTRDIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            16429..17898
                     /gene="lysP"
                     /locus_tag="ACHUQ7_001436"
     CDS             16429..17898
                     /gene="lysP"
                     /locus_tag="ACHUQ7_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFR1134915.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            18190..20181
                     /gene="cirA"
                     /locus_tag="ACHUQ7_001437"
                     /pseudo
     CDS             18190..20181
                     /gene="cirA"
                     /locus_tag="ACHUQ7_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(20213..21048)
                     /gene="yeiG"
                     /locus_tag="ACHUQ7_001438"
                     /pseudo
     CDS             complement(20213..21048)
                     /gene="yeiG"
                     /locus_tag="ACHUQ7_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
     gene            21306..21974
                     /gene="folE"
                     /locus_tag="ACHUQ7_001439"
     CDS             21306..21974
                     /gene="folE"
                     /locus_tag="ACHUQ7_001439"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFR1134916.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            21991..23067
                     /gene="yeiB"
                     /locus_tag="ACHUQ7_001440"
     CDS             21991..23067
                     /gene="yeiB"
                     /locus_tag="ACHUQ7_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFR1134917.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLGVLLLLGLISDSQTSRAWTPDASAILYEKYWKLHGGVEAISNRA
                     DGVGNSLLALGAQYGWQLAGMMLIGAALMRSGWLKGQFSLRHYRRTGFVLVAIGVTIN
                     LPAIALQWQLDWAYRWCAFLLQMPRELSAPFQAIGYASLFYGFWPQLSRFKLVLAIAC
                     VGRMALTNYLLQTLICTTLFYHLGLFMHFDRLELLAFVIPVWLANILFSVIWLRYFRQ
                     GPVEWLWRQLTLRAAGPAISKTSR"
     gene            23209..24249
                     /gene="galS"
                     /locus_tag="ACHUQ7_001441"
     CDS             23209..24249
                     /gene="galS"
                     /locus_tag="ACHUQ7_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFR1134918.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            24529..25527
                     /gene="mglB"
                     /locus_tag="ACHUQ7_001442"
     CDS             24529..25527
                     /gene="mglB"
                     /locus_tag="ACHUQ7_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFR1134919.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            25588..27108
                     /gene="mglA"
                     /locus_tag="ACHUQ7_001443"
     CDS             25588..27108
                     /gene="mglA"
                     /locus_tag="ACHUQ7_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFR1134920.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            27124..28134
                     /gene="mglC"
                     /locus_tag="ACHUQ7_001444"
     CDS             27124..28134
                     /gene="mglC"
                     /locus_tag="ACHUQ7_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFR1134921.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKISGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(28170..29383)
                     /gene="preA"
                     /locus_tag="ACHUQ7_001445"
                     /pseudo
     CDS             complement(28170..29383)
                     /gene="preA"
                     /locus_tag="ACHUQ7_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
     gene            complement(29377..30615)
                     /gene="preT"
                     /locus_tag="ACHUQ7_001446"
     CDS             complement(29377..30615)
                     /gene="preT"
                     /locus_tag="ACHUQ7_001446"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HFR1134922.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEQQRNTIKTQNYQIRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
BASE COUNT         7459 a         7474 c         8154 g         7683 t
ORIGIN      
        1 gagtggcgat aatcatcacc atcgcaaacc agaacacagc gtgataattc cagatctcgg
       61 cgacgatgcc cgccactgat cctgcgatga tccagcccac gcgcgacgtg ttggtataga
      121 gcgtggtggc tgaacctgcc tgaccgggca tcaaatcctg aaaatagagc atcccgatgc
      181 cgcccagaat gccaataaaa atagcattta gcagctgcaa gcccaacaga atggcaggtg
      241 aatgcgccat cagcattcct gcgtaaaaac agacgccacc cacggcagca acgcgcatta
      301 agaaacgctt acccagacgt ttggcgaaat atccggcaat caacatcgtc gggatttcca
      361 gcccggcggc ggtccccatc atcacaccgg ccagtttctc gggaagatgc agttcgttga
      421 taataaatag cggcatgttg atgatgtaca ggctgttcga gccccacatc aatgtacaaa
      481 tgacaaacag cagtagcgta tcgcgacggt tacggcgcgg cgcttcaacc gtgccggtcg
      541 ccagcggaag ctctttttgc atcgacggta aaaacagcca caccatcacg ccgcaaacaa
      601 taaacgctac cgctgcgctc agatacatta ccgtaaagct gaaacccatc gctaacgcat
      661 aagccagcgg tgggccaatg acccatgcca gtgaaacctg agcgcgtaaa aaagagctga
      721 acatcaccgc ctcacgtccg gttttgtcgg catgttcacg ggcaagggca aacatttgcg
      781 ggttagcggt cgagccaaag ctgctaagaa agacgccaac gaatagcaaa acaaagtagt
      841 tgcgattcca agcaaaaagg gtgcaggcca gcacgcctaa caggcagcaa aagacaatca
      901 gcgatttgcg atcgccgcgc ttatcagagc gcccagcgag aaactgactt accagaatcc
      961 caatgacagc gctgccggta aagaagaatc ccaccatcgc cggacgggca tgtacttcat
     1021 cggtaaggaa aatactgagt gtcggggttt gcagagcacc cgcaataccg gtgagaaagg
     1081 caacgattaa aaacgccgtc gaggtcaggt caaacgattt cgcgctggag actgcggggg
     1141 agttatgcat gctggtcgat cactggaaga ggaagacgcg gagtttacgc tgcttgtcag
     1201 aaaataaaca gacagcgaaa tcaaaagcgc cacgaataat caaaatgcca tttcactgtt
     1261 gctgattggc tgctgacgct gctgaaatga aattgctgat aacggatttt cccatcagca
     1321 attaggaaaa atgacaaaaa attgtgcagc acatcaaact tttgctcata actttacggc
     1381 tttccttgcg tgctgaaaca gaagtattat gctttcttga aacgtttcag cgcgatcttg
     1441 tctttaaccc taagctaggt tggcgctttt tttctcatag aggctgaatc gtttcaattc
     1501 agcaagagag gagaactatg ttccagttat ccgtacagga catccatccg ggcgaaaagg
     1561 ccggagacaa agaagaggcg attcgccagg tcgctgcggc gctggtgcag gccggtaatg
     1621 tagcagaagg ctacgtcaat ggcatgctgg cgcgcgaaca gcaaacctca acgttcctcg
     1681 gcaatggtat tgctattcca cacggcacta ccgacacccg cgatcaggtg ctgaaaaccg
     1741 gcgttcaggt atttcagttc ccggaaggcg tcacctgggg tgacggtcag gtagcgtacg
     1801 tggcaatcgg tattgctgcc agctcggata aacatctggg cctgctacgc cagctgaccc
     1861 acgtactgag cgatgattcc gttgctgaac aactgaagtc agcaacaaca gcagaagaac
     1921 ttcgcgcatt actgatgggc gaaaagcaga gtgagcagct gaagctcgac aacgaaatgc
     1981 tgacgctgga tatcgtcgcc agcgatctgc tgactcttca ggcgctgaac gctgcgcgtc
     2041 tgaaagaggc gagggcagtt gacgccactt tcgtcaccaa agccatcaat gaacaaccgc
     2101 tgaatctcgg acagggtatc tggctgagcg atagcgccga aggcaatctg cgtagtgcga
     2161 ttgcggtaag ccgtgcggca aatgcttttg atgtggacgg cgaaacggca gccatgctgg
     2221 tgagtgtggc gatgaatgac gatcagccca tcgcggttct taagcgtctc gctgatttgt
     2281 tgctcgacaa taaagctgac cgcttgctga aagcggatgc ggcaacgttg ctggcgctgc
     2341 tgaccagcga tgatgcgccg accgacgacg tgttaagcgc ggagtttgtg gtgcgcaatg
     2401 aacacggcct gcatgctcgt ccaggtacca tgctggtcaa taccattaaa caatttaaca
     2461 gtgatattac cgtgacaaac cttgatggca ccggcaaacc ggcaaacgga cgtagtctga
     2521 tgaaagttgt ggcacttggc gttaagaaag gtcatcgcct acgctttacc gcccagggtg
     2581 cagatgctga acaggcgctg aaagccattg gcgacgctat cgctgctggt cttggggagg
     2641 gcgcataatg agcagacgtg ttgctacgat cacccttaat ccggcttatg accttgttgg
     2701 tttctgcccg gaaattgaac gcggcgaagt gaacctggtg aaaaccaccg gtctgcatgc
     2761 ggcgggtaaa ggcatcaacg tggccaaagt attaaaagac ctgggaattg atgtcaccgt
     2821 tggcggcttc ctgggtaaag acaatcagga tggttttcag caactgttca gcgagctggg
     2881 cattgccaac cgtttccagg ttgtacaggg gcgcacccga attaacgtta agctgacgga
     2941 aaaagacggc gaagtgaccg acttcaactt ctcgggtttc gaagtcaccc ccgccgactg
     3001 ggaacgcttt gtgactgatt ctctgagctg gctcggtcag ttcgatatgg tctgtgtcag
     3061 cggaagctta ccgtcaggcg tcagcccgga agcgttcacc gactggatga ctcgcctgcg
     3121 tagtcagtgt ccttgcatta tctttgatag tagccgtgaa gcgttagtag caggtttgaa
     3181 agcggcaccg tggctggtga aacctaaccg ccgcgagttg gaaatctggg caggccgtaa
     3241 actgcctgaa atgaaagatg tgattgaagc tgcacatgcg ctacgtgaac aaggtattgc
     3301 gcatgttgtt atttcactgg gtgccgaagg cgcgctttgg gttaatgcct ccggcgaatg
     3361 gatcgctaaa ccaccgtcag tcgatgtcgt aagcaccgtt ggcgcagggg attctatggt
     3421 tggtggcctg atttatggct tgctgatgcg tgaatccagt gaacacacac tgcgtctggc
     3481 gacagctgtt gcagccctgg cggtaagtca aagcaatgtg ggtattaccg atcgtccgca
     3541 gttggccgca atgatggcgc gcgtcgactt acaacctttt aactgacagc aggagaggca
     3601 taatgaaaac gctgctgatt attgacgcta atctcggtca ggcacgcgcc tatatggtga
     3661 agaccctgct gggcgcggcg gcgcgaaaag caaaactgga aatcatcgac aatccgaacg
     3721 acgcagaaat ggcgattgtt ctcggtgatt ccatcccgaa tgacagcgcg ctgaacggta
     3781 aaaatgtctg gctgggtgat atttcccggg cagttgcgca ccctgagctg ttcctgagtg
     3841 aagccaaagg ccatgcgaaa ccttacactg cgccggtcac tgcgacagca ccggttgccg
     3901 ccagcggtcc gaaacgcgta gttgcggtga ctgcttgccc gactggcgta gcacacacct
     3961 ttatggcggc tgaagccatt gaaaccgaag cgaaaaaacg tggctggtgg gtgaaagttg
     4021 aaacccgtgg ttctgttggc gcgggtaatg caatcactcc cgaggaagta gccgcagcgg
     4081 atctggtgat tgtggcggca gatatcgaag tggatctggc gaaatttgct ggtaaaccga
     4141 tgtatcgtac ctctaccggt ctggcgctga agaaaacctc gcaggaactg gataaagcgg
     4201 ttgctgaagc aacgccgtat gaaccggcgg gcaaagctca aacggcgacc actgaaggta
     4261 agaaagagag tgcaggcgct tatcgtcact tgctaacggg cgtctcttac atgctgccga
     4321 tggtcgttgc tggtggtctg tgtatcgcgc tttcttttgc ttttggtatc gaagcgttta
     4381 aagagccggg tacgttggca gcggcgctga tgcagattgg tggtggttca gcctttgcgc
     4441 tgatggtgcc ggtactggca ggttatattg ccttctccat tgccgatcgt ccgggcctca
     4501 cgccgggtct gattggcggt atgctggcgg tcagcaccgg ttctggcttc attggcggta
     4561 ttattgcggg cttcctggct ggttacattg cgaagttaat cagtacgcaa ctgaaactgc
     4621 cgcagagtat ggaggcgctg aaaccgatcc tgatcattcc gctaatttcc agtctggtgg
     4681 tcggtctggc gatgatctac ctgatcggta aaccagttgc tggcattctc gaagggctga
     4741 ctcactggct gcagaccatg gggactgcga atgcggttct gctgggggcg atcctcggtg
     4801 gcatgatgtg tactgacatg ggcggtccgg taaacaaagc agcgtacgca ttcggtgtgg
     4861 gtctgctgag tactcaaacc tatggcccga tggcggcgat tatggcggca ggtatggtgc
     4921 caccgctggc aatgggcctg gcaacaatgg tggcgcgtcg caaattcgac aaagcgcagc
     4981 aggaaggtgg caaagccgct ctggtattgg gactgtgctt catttcggaa ggtgcaattc
     5041 cgtttgctgc tcgtgatccg atgcgtgtgc tgccgtgctg tatcgtgggt ggggcgctga
     5101 ctggcgcaat ctcaatggcg attggtgcga aactgatggc accgcacggt ggtctgtttg
     5161 ttctgctgat ccctggcgct attacgccgg tattgggtta cctggtagca attattgccg
     5221 gtacgctggt ggcgggtttg gcctatgcct tcctgaaacg tccggaagtg gacgcagtag
     5281 cgaaagcagc gtaataaaag gtgtgttaca gggcagaaat ttcctgccct gtaacatttg
     5341 atgagcgcaa agagaatgac cagcttgtga atgtaatttt atgaatttaa atggttttat
     5401 ctttcagtag aaacttcaaa cgttcatcaa acactaaggt tatttcctca agggcagatt
     5461 attttctgcc cttttttagt gttcctgttc tgtgcatccc gtcacaaatt cctcgctttt
     5521 gtaattcagg catttaatcg cattttgtga gcctgctcaa atctgcccat tttgcctctc
     5581 tggcggtaat ttactttacg taaaacatgc atactatgag cacatgttta aatatgaaca
     5641 catgtttaaa ccgtctttgc agtatctata ttcttgtgga ttgacagggt tggtaatttt
     5701 ttaccagagg acgtgctatg cgcgaaaagg attatgtcgt aattataggt tcggcgaata
     5761 ttgatgtcgc cggatattca catgaatcat taaattatgc ggattcaaat ccaggtaaaa
     5821 taaaatttac gcctggtgga gtagggcgca atattgcaca aaacctggcg ttgctgggta
     5881 acaaagcctg gctactgagc gccgtaggca gtgattttta tggtcaatcg ctgctaacgc
     5941 aaaccaatca atctggtgtt tatgtcgata aatgcctgat tgtgccggga gaaaatacgt
     6001 cgagttattt atcattactc gataataccg gtgaaatgct ggttgctata aatgacatga
     6061 atattagcaa cgctattaca gctgaatatc tcgcacagca ccgtgaattt attcagaggg
     6121 caaaggtcat tgtcgcggac tgtaatatca gtgaagaggc actggcatgg attctggata
     6181 atgccgccaa cgtacccgta tttgtcgatc cggtttcagc gtggaaatgt gtcaaagtgc
     6241 gcgaccgtct aaatcagaac cacactctga agccaaaccg ccttgaagcg gaaaccctga
     6301 gtgggattgc gctgtcaggg cgtgacgatg tggcaaaagt tgctgcctgg ttccatcaac
     6361 atggcctgaa ccgactggta ttgagcatgg gcggcgacgg cgtttattac agcgatatca
     6421 gcggtgaaaa tggctggtct gcgccgatca aaaccaatgt tattaatgtt accggagcgg
     6481 gcgatgccat gatggcggga cttgcttcgt gttgggtaga cggaatgccg tttgccgaat
     6541 ctgttcgttt cgcacaggga tgttcgtcaa tggcgctctc ctgtgaatac accaataacc
     6601 ccgatttatc gattgccaac gttatatcgt tagtggagaa cgcagaatgt ctgaattaaa
     6661 aatttcccct gaattattac aaatttctcc ggaagtgcag gacgctttaa aaaacaaaaa
     6721 accggttgtg gcgctggaat cgaccattat ttctcacggg atgccgttcc cacaaaatgc
     6781 ccagaccgca attgaagtag aagaaactat tcgtaaacag ggcgcagtac ctgccactat
     6841 cgccattatt ggcggcgtga tgaaagtggg tttaagcaaa gaaaaaattg aattactggg
     6901 tcgtgaaggg cataacgtga ccaaagttag tcgtcgcgat ttaccttttg ttgttgccgc
     6961 cggaaaaaat ggcgcaacca ctgtggcttc aacgatgatt attgcggcgc ttgccggaat
     7021 taaagtattt gccaccgggg gaattggtgg tgtgcatcgc ggggcggaac ataccttcga
     7081 tatttctgcc gatttgcaag aactggcaaa tactaatgtc accgttgttt gtgccggggc
     7141 gaaatctatt ctcgatttag gattaaccac tgagtattta gaaaccttcg gtgtgccgtt
     7201 aattggctat cagactaaag cgctgcctgc gtttttctgc cgtaccagct cgtttgacgt
     7261 cagcattcgt ctcgacagcg ccagtgaaat tgcccgtgca atggcggtga aatggcaaag
     7321 cgggctgaac ggtggcctcg tggtagcgaa cccgatcccg gaacagtttg cgatgccgga
     7381 ggaatctatc aatgcagcca tagatcaagc cgtcgccgaa gccgaagagc agggcgttat
     7441 tggtaaagaa agtacaccgt tcctgctggc gcgcgttgct gagctgacca gcggtgacag
     7501 cctgaaatcc aatatccagc tggtgttcaa caacgccatt ctggcgagtg aaattgccaa
     7561 agaataccag cgtctcgcgg gttaatttct gacgggcaga aagccgtcgc ccgtactcat
     7621 caagcatacc aaaacctggc gctgacgccg ggtttactgg catgaaggga aaatcattat
     7681 ggatataatg agaagtgttg tggggatggt ggtgttactg gcaatagcat ttctgttgtc
     7741 agtgaataaa aagagcatca gtttgcgcac ggttggagcc gcactgctgc tgcaaatcgc
     7801 tattggtggc atcatgctct acttcccacc gggaaaatgg gcagtagaac aggcggcatt
     7861 aggcgttcat aaagtgatgt cttacagtga tgccggtagc gccttcattt ttggttcgct
     7921 ggttgggccg aaaatggatg tcctgtttga cggtgcgggt tttatcttcg cctttcgcgt
     7981 acttccggcg attattttcg ttactgcgct catcagtctg ctgtactaca ttggcgtgat
     8041 ggggctgctg attcgcatcc ttggcagcat tttccagaaa gccctcaaca tcagcaaaat
     8101 cgaatctttt gttgcggtta ccactatttt cctcgggcaa aatgagatcc cggcgatcgt
     8161 taaaccgttt atcgatcgcg tgaatcgcaa cgagttgttt accgcaattt gtagcgggat
     8221 ggcgtccatt gctggttcgg tgatgattgg ttatgccgga atgggcgtac caattgacta
     8281 cctgttagcg gcatcgctga tggcgatccc cggcggtatt ttgtttgcac gtattcttag
     8341 cccggcaacc gagccttcgc aggtcacatt tgaaaatctg tcgttcagcg aaacgccgcc
     8401 aaaaagcttt atcgaagcgg cggcgagcgg tgcgatgacc gggctgaaaa tcgccgctgg
     8461 tgtagcgacg gtggtaatgg cgtttgtcgc aattattgcg ctgatcaacg gcattatcgg
     8521 cggaattggc ggctggtttg gtttcgccaa tgcctctctg gaaagtattt ttggctatgt
     8581 gctggcaccg ctggcgtgga tcatgggtgt ggactggagt gatgccaacc ttgctggtag
     8641 cctgattggg cagaaactgg cgattaacga attcgtcgct tacctgagtt tctccccata
     8701 cctgcaaacg ggcggcacgc tggaagtgaa aaccgtggcg attatatcct ttgcgctttg
     8761 tggttttgct aactttggtt ctatcggtgt tgtcgttggc gcattttcgg ctatttcgcc
     8821 aaaacgtgct ccagaaatcg cccagcttgg tttacgggcg ctggcagcag caacgctttc
     8881 caacctgatg agtgcgacta ttgccgggtt ctttattggt ctggcgtaat gctcgatttg
     8941 tagaccggat aaaacgtatt aagcgttgca tccggcaatc gcgctattgc aaataatttt
     9001 tactgcatca tcccggagaa tttattctcc gggtccatct ccagcgccag tcctgacaac
     9061 tgctttctgt ttttaattag atatcctttc ttttaattaa taaaccgtcg tgaatgaatt
     9121 gcgcgagaac atataaaaga tgtcgataag aaacgcctaa atactctgcc gcttgcgtat
     9181 gcttttcgtg ataaagatca ccttcttgtg agagtaatat aaatgctgcc aggcgattca
     9241 ctaacggaaa tgattgattc tgagttaaag aaacaatatt acgataattt ttatgactta
     9301 aggtgacgca aagttttcgt aaaaataggg tgtcgtttaa taacagcgga cggtaatgtt
     9361 tcatagggag cgcaaggcac caacactctt caatagcctg caccgcacgc ggttcatggt
     9421 ctttgtcgat taactcaatc tcgccaataa aacagggagc ggcaaagaaa tcgatcagcg
     9481 acacgcgacc attggcaagc gtggcgtaaa gcctggcgcg gcctcgcgtc agataaaaca
     9541 gccatgacgg ctgttgccct tcctgcacaa tgtagtcacg cgctaaaaaa tgaaacagcc
     9601 gcgtatcggc tgaaacatcc gttgaaaagc aatccttaaa cgcagattca ctcatcaatt
     9661 gctgcttaag gtcattattg tggatttctt tcacgatgtt tatccgctat gagatttctc
     9721 atatttagca tacgtacgct gttgttaaga ttcaatcatt ggcccggtcg aattttcttt
     9781 gctgtagaaa aaaataacgc aactggaaac agaggaaata aacaatggaa aagagaaaaa
     9841 ttattctgga ttgtgagccg ggtcatgatg atgctattgc aatgatgatg gcagcgaaac
     9901 atccggcaat agatttatta ggcatcacta ttgtagcggg taatcagacg cttgataaaa
     9961 cattaattaa tggcctgaat gtttgccaga aactggagat taatgttccg gtttatgcgg
    10021 ggatgccgca acccattatg cgtaaacaaa tcgttgccga taatattcac ggtgaaaccg
    10081 gactggatgg cccggtattc gagccgctga cccgccaggc agaaagtacc catgcggtga
    10141 aatatatcat cgataccctg atggcaagcg atggcgatat cactctggtg ccggttggtc
    10201 cgctttcaaa tatcgcggtg gcaatgcgta tgcaacccgc aatcctgccc aaaatccgtg
    10261 aaattgtgct aatgggcggc gcttacggta caggcaactt caccccatct gccgagttca
    10321 atatctttgc cgacccggaa gccgcccgcg tggtgttcac ctccggcgtt ccattagtga
    10381 tgatgggcct cgatcttacc aaccagaccg tttgcacccc ggatgtaatt gctcggatgg
    10441 aaagggtagg cggccctgcc ggagagctgt tcagcgacat catgaacttc actctcaaaa
    10501 cgcagttcga atactacggc ctggctggcg gcccggtgca tgacgccacc tgcattggtt
    10561 atctgatcaa ccctgatggc attaaaaccc aggatatgta cgtcgaagtg gacgtcaaca
    10621 gcggcccttg ctacgggcgt acggtctgcg acgaactggg cgttcttggc aagcccgcta
    10681 ataccaaagt cggcatcact attgataccg actggttctg gggcttagtc gaagagtgcg
    10741 tgcgcggcta catcaaaacc cattaagtgt tgataacaag ccgggacagt atcgcaagat
    10801 gcaggcccgg ctatttctga tgggcagaaa gccgtcgccc aaaatgacat gaagagataa
    10861 tcactatgga tgtcatgaga agtgttctgg gaatggtggt actgctggca attgcgtttt
    10921 tactgtcagt aaacaagaag aagatcagcc tgcgtaccgt tggcgcggcg ttagtgttac
    10981 aggtcgtgat tggcgggatt atgctttggt taccgccagg gcgttgggtc gcagaaaaag
    11041 tcgcttttgg cgtccacaaa gtgatggcgt acagcgacgc aggtagcgca tttatcttcg
    11101 gttctctggt cggaccgaaa atggatacct tatttgatgg cgcaggattt atctttggtt
    11161 tcagggtatt accggcaatt atcttcgtca ctgcactggt gagtattctc tactacatcg
    11221 gtgtgatggg gatcttaatt cgcattctcg gcggtatctt ccagaaagct ttaaatatca
    11281 gcaagatcga gtcattcgtc gcggtcacca ccattttcct cgggcaaaac gaaattccgg
    11341 cgatcgtcaa accctttatc gatcgtctga atcgcaatga attatttaca gcgatttgta
    11401 gtggcatggc ctcgattgct ggttcgacaa tgattggtta cgccgcactg ggcgtacctg
    11461 tggaatattt gctggcggca tcgttaatgg cgatccctgg cgggatcttg tttgcccgcc
    11521 tgttaagccc ggctacggaa tcttcgcagg tttcttttaa taacctctct ttcaccgaaa
    11581 caccgccaaa aagcattatt gaagccgatg cgacaggggc aatgaccggg ctgaaaatcg
    11641 ccgccggtgt agcgacagtt gttatggcat ttgtcgccat cattgcgtta attaacggta
    11701 ttatcggcgg cgttggcggc tggtttggtt ttgaacatgc ctcgctggag tccattttag
    11761 gttacctgct ggccccactt gcgtgggtaa tgggtgttga ctggagtgat gcaaatcttg
    11821 ccgggagttt gattggacag aaactggcaa taaatgaatt tgtcgcttat ctcaatttct
    11881 caccctatct gcaaacggct ggcactctgg atgctaaaac cgtggcgatt atttccttcg
    11941 cgttgtgcgg tttcgctaac tttggttcta tcggggtggt ggtgggggcg ttttctgcgg
    12001 ttgcgccaca ccgtgcgccg gaaatcgccc agcttggttt acgcgcgctg gcggcggcga
    12061 cactttctaa tctgatgagt gccaccattg ccgggttctt tattggttta gcgtagtgtg
    12121 caatttatga gccggattag atgcggtcaa gcgtcgaatc cggcaacatt gttacaccat
    12181 tggcactaat gacagcgcgt tatcggcaga cagggtaggg ttgtttaagc tgccgctggc
    12241 gcgtgaaatt gccgcgcagg ccatcgcaaa acgggcgctg tcacggaaat tgtttccctc
    12301 cagaaagcta tataccagac ccgccataaa accatcgtca gcaccaaaac tgtctaccgt
    12361 cgtatgcgct ggcacagtca gcaaaaattg ttcaccatcc ttttcgctgc aataaactga
    12421 ctcatcgggc aaataaacaa acagttgctg aacaccttgc tgatgcaatg cattcactgc
    12481 ggcattacgg tcagcatcgc tggtgatcgc ctgtccccat aaaatttcca gctccggtaa
    12541 agtgggtttc agggtgtgaa tatgcgccag ccagtgtttg attttgcccg ctttgaattc
    12601 tgaaacggta tcgacaaaca ccgggatctc atcagcgagg gtaaagaccc attccagcgc
    12661 ctcggctgtc aggttgcaat cagccagtac cacgcccgca tgacgaagta aatcgcgcga
    12721 cccgttcagt agttgcggtg tcaactgttc cagcagatgg gtatcgttaa ttgccagcac
    12781 ggtttgatcg tctcgattgg caattgccag atacgtcgat gtgctttgac catgcaaacg
    12841 aacgcagccg gagacattca cgccggcacg gcgcgtttct tccaggagca tttcgccata
    12901 aaagtcatcg ccaatcactg aaagcaaatg gacgtcacgg cctaacagcg ccagattgtg
    12961 ggcgatattg cgtccaacgc cgcctgccga acaatgaatt gtaccgggat gagaagccgc
    13021 ttgcgggtaa tggatatccg ccatcccgcg aatatccata ttgattgtcc ccaccactac
    13081 gcagtattcc tgctcggtga gaatgtaacc tttgtcttta atccggcctt tgcgcattaa
    13141 atccataata tgcgccgcaa cgcgcgaacg actgatttgc agcatgtccg caatttcgtt
    13201 ctgctgaatc agcgggttac gccgtaaaat tgcaaggatc tccttttccc ggttatttat
    13261 cttcaggcta ccgctttttc agtttgttgc gctttcagcc aggcgatctc ttctgcccag
    13321 atatccgggt tgatggtttc aaggatcagc ggaatgccgt cgaaacggtc gttctgcata
    13381 atccagcgga acgcatcatg accgatattg ccttcaccga ggctatgatg gcggtcaacg
    13441 cggctgccaa aggtactttt cgcatcgtta aggtgcatcc cgcgcaaata cttaaagccg
    13501 acaatacggg caaaatcagc gaacgttttt tcgcattcgg ctggagtacg caaatcatac
    13561 ccggcagcga aagcatggca ggtatcaatg cagacgccga cgcgggattt atcttccacg
    13621 ccgtcgataa tcgccgcgag atgttcgaat ttaaacccta agttactgcc ctgaccggcg
    13681 gtgttttcaa tcaccgctgt tacaccttga gttttatcca gcgcaatgtt gatggattcg
    13741 gcaatacgcg caaggcaatc ctcttctgaa atctgcatca gatggctgcc agggtggaag
    13801 ttgagcaaag aaagccccag ttgttcgcaa cgctgcattt catctataaa ggcatcgcgc
    13861 gatttttcca gagcttcagt gaccggatgt ccgaggttga tcagataact gtcgtgggga
    13921 agaatttgcg ccgatgtgta gtgatatttt tcacaggcgg ctttgaattc atcgatggtt
    13981 tgcgtcgtga gcggtgcggc acgccactga cgttggtttt tggtgaacaa ggcaaacgcg
    14041 gttgcgtcga tttcggcggc gcgaattgcg gcatttgcca gaccgccagc agcactaacg
    14101 tgcgctccaa tgtatttcat gcgaggactc ctgttaaacc cgctggagga aaacggtaat
    14161 gatagcgggt taacaggagc aagaagtagt ggtttatgcg atgacgcttt gaatcacata
    14221 gttaatcgca ccaccaccaa caatcagcca ggcaaacagt accagtgcca tcagcagagg
    14281 tttcgcccca gcttttttca gcgcgctgac gtgagtggtc agacccagcg ccgccatcgc
    14341 cattgccagc aggaaggtat ccagcgttac cagcatgttc accacgctct gcggtaacag
    14401 gtggaacgag ttaaagatgg caactacgat gaacaagatg gcaaaccacg gaatagtgat
    14461 tttgcttttc tcgccgctgt tcgccccaga cagctgttta acacgcgccg ccagcaggat
    14521 gaggaacgga gccagcatca tcacgcgcag cattttggaa ataactgctg cgttttctgc
    14581 atccgggctg atggcatgac ctgccgccac cacctgcgcc acttcgtgca cagtagaacc
    14641 aatgtagata ccgaaagttt ccggactaaa ccattgagac atcagcggat atatcgccgg
    14701 gtagaggaaa atcgcgacgg tcccgaagat aacaacggtt gcaacagcca cggttacttt
    14761 actggcttcc gctttcacta ccggctcagt cgccagtacc gcggcagcac cacagatact
    14821 gctaccggca ccgatcaacc aactggtgtg cttatccaga ccaaacactt tctgccccag
    14881 gaagcaagcc agcaggaagg tactggacag cgtcagcacg tcaatgatga tcccactgat
    14941 accgacatcg gcaatttgcg agaacgtcag acggaagcca taaagaatga tacccagacg
    15001 taataaatat tgcttggcaa acagcacacc accgtcacag cttttccaga tgtgcggata
    15061 gatggtgttg cctaaaacca tccccaacaa gattgcgagg gtgagggcac taaacccggc
    15121 acccgcaacc gcgggaatgg aaccacccca cagggcgacc ccggtgataa ctgcactcag
    15181 ggctaacccc ggaataaaat gccacagtgt acgatgttgt ttctgtaagg tgatattcgt
    15241 cataaccctc tcctttaacc gggcataagg ttacgactaa ctggtttaaa aataaaattg
    15301 attatatatt tataattaat ctttataagt ggtaagcgac tatgcacatc accctccggc
    15361 agttggaagt ttttgcagaa gtattgaaaa gtggatcaac cacccaggcg tcggtgatgc
    15421 tggcgttgtc gcaatcagca gtgagcgcag ccttgactga cctggaaggg cagcttggcg
    15481 ttcaactgtt tgatcgcgtg gggaaaagac tggttgttaa tgaacacggg cggctgctct
    15541 atccgcgagc gttggcactg cttgaacagg cggttgaaat cgaacaactg tttcgcgaag
    15601 acaacggcgc gattcgtatc tatgccagta gtaccatcgg taactacatt ctgcctgcag
    15661 ttatcgcccg ttatcgccat gattatccgc agttgccgat tgaacttagc gttgggaaca
    15721 gccaggacgt gatgcaagcg gtgctggatt tccgcgttga tattggcttt attgaaggac
    15781 cgtgccacag cactgaaatc atttctgaac cgtggctgga agacgagctg gtggttttcg
    15841 ccgcgccgac ttcgccgttg gctcgtggtc cggtcacctt agaacagctg gccgctgcgc
    15901 cgtggatcct gcgtgaacgc ggttccggca cgcgggatat tgtcgattat ctgttgctgt
    15961 cacatttacc gaagtttgag atggcgatgg aattaggtaa ctccgaggca atcaaacatg
    16021 cggtgcgtca tgggttggga attagttgcc tgtcgcgacg tgtgattgaa gatcaattgc
    16081 aggcaggcac attaagtgaa gttgcggtcc ctctgccgcg cctgatgcgt acgttgtggc
    16141 gtatacatca tcggcaaaaa cacctttcca acgcgctacg gcgctttctg gactattgcg
    16201 atcccgcaaa tgtgccgcgt taagttgctg tacaagaaca tgctggtgct gtgtcgattt
    16261 cgtgacgcag cgccttcagc atgcattcgc cagaaaagag attggctgct ttacttataa
    16321 tccctgggcg atcatgaagg tgtcttataa ccgtgtattt ctgccggaag gattgccaat
    16381 cgtctgctac aatcgcgcca tcatttttaa gatggatagc atttttgtat gggttccgaa
    16441 actaaaacta cagaagcgcc gggcttacgc cgtgaattaa aggcgcgtca cctgacgatg
    16501 attgccattg gcggttccat cggtacaggt ctttttgttg cctctggcgc aacgatttct
    16561 caggcaggtc cgggcggggc attgctctcg tatatgctga ttggcctgat ggtttacttc
    16621 ctgatgacca gtctcggtga attggctgca tatatgccgg tttccggttc gtttgccact
    16681 tacggtcaga actatgttga agaaggcttt ggcttcgcgc tgggctggaa ctactggtac
    16741 aactgggcgg tgactattgc cgttgacctg gttgcagcac agctggtcat gagctggtgg
    16801 ttcccggata caccgggctg gatctggagt gcgttgttcc tcggcgttat cttcctgttg
    16861 aactacatct ctgttcgcgg ctttggtgaa gcggaatact ggttctcact gatcaaagtc
    16921 acgacagtta ttgtctttat catcgttggt gtgctgatga ttatcggtat cttcaaaggc
    16981 gcgcagcctg cgggctggag caactggaca atcggcgaag cgccgtttgc tggtggtttt
    17041 gcggcgatga tcggcgtagc tatgattgtc ggcttctctt tccagggaac cgagctgatc
    17101 ggtattgctg caggcgagtc cgaagatccg gcgaaaaaca ttccacgcgc ggtacgtcag
    17161 gtattctggc gaatcctgtt gttctatgtg ttcgcgatcc tgattatcag cctgattatt
    17221 ccgtataccg atccgagcct gctgcgtaac gatgttaaag acatcagcgt cagtccgttc
    17281 accctggtgt tccagcacgc gggtctgctc tctgcggcgg cggtgatgaa cgcagttatt
    17341 ctgacggcgg tgctgtcagc aggtaactcc ggtatgtatg cgtctactcg tatgctgtac
    17401 accctggcgt gtgacggtaa agcgccgcgc attttcgcta aattgtcgcg tggtggcgtg
    17461 ccgcgtaatg cgctgtatgc gacgacggtg attgccggtt tgtgcttcct gacctccatg
    17521 tttggcaacc agacggtata cctgtggctg ctgaacacct ccgggatgac gggttttatc
    17581 gcctggctgg ggattgccat tagccactat cgcttccgtc gcggttacgt attgcaggga
    17641 cacgacatta acgatctgcc gtaccgttca ggtttcttcc cactggggcc gatcttcgca
    17701 ttcattctgt gtctgattat cactttgggc cagaactacg aagcgttcct gaaagatacc
    17761 attgactggg gcggcgtagc ggcaacgtat attggtatcc cgctgttcct gattatttgg
    17821 ttcggctaca agctgattaa aggaactcac ttcgtacgct acagcgaaat gaagttcccg
    17881 cagaacgata agaaataagt ttcctccctt ccttgctaag ccctctcaac cgagagggct
    17941 ttttcaattc catttccctg acaaatcatg cggatataaa atttaacatt tggattgata
    18001 attgttatcg tttgcattat cgttacgacg caatcaaaaa aggctgacaa atcagaggct
    18061 gttccggctt tctgggatga tcacctgcat acaaataagt ccaccgcgat gctgccgtac
    18121 gcaaggggac gtaaagaaga tgtgagcgat acccatttta ttttcgtagt tacctcatgg
    18181 agatatggaa tgtttaggtt gaaccctttc gtacgggtcg ggctgtgttt gtccgctatt
    18241 tcttgtgcat ggcctgtgtt agcggtcgat gatgatggcg aaacgatggt tgtcactgca
    18301 tcttccgtgg aacaaaatct taaagatgca cccgccagta tcagcgtcat tacccaggaa
    18361 gacctgcagc gaaaaccggt acagaatctg aaggatgtcc tcaaagaagt gcctggcgta
    18421 caactgacga acgaagggga taaccgtaag ggcgttagta ttcgtggtct ggacagcagc
    18481 tacaccctga ttcttgtcga cggtaaacgc gttaactccc gcaatgccgt cttccgccac
    18541 aatgatttcg atctgaactg gatcccggtc gattccatcg aacgtattga agtggttcgt
    18601 ggcccgatgt cgtcgctata cggctccgat gcgctcggcg gtgtagtgaa tatcatcacc
    18661 aaaaaaatcg gtcagaaatg gtcgggtacc gttaccgtcg ataccaccat tcaggaacat
    18721 cgcgatcgcg gtgataccta taacggtcag ttctttacca gtggaccatt aattgatggt
    18781 gtgctgggaa tgaaagctta cggcagcctg gcaaaacgtg aaaaggatga cccgcaaaac
    18841 tcaacgacca ccgataccgg agaaacgccg cgtattgaag gattctccag ccgcgacggc
    18901 aatgtcgaat ttgcctagac accgaatcaa aatcacgatt ttactgccgg atacggtttc
    18961 gaccgtcagg atcgtgattc cgattcgctg gacaaaaacc gcctggaacg ccagaactac
    19021 tgcgtcagcc ataatgggcg ttgggattac ggcaccagcg aactgaaatc ctacggtgag
    19081 aaagtcgaga acaaaaaccc tggcaacagc agcccgataa cttccgaaag caatacggtc
    19141 gacggcaaat acacgttgcc gctgacggcg attaatcagt ttctcacggt tggcggtgaa
    19201 tggcgtcacg acaaacttag cgatgcggtg aacctgaccg ggggaaccag ctccaaaacg
    19261 tctgccagcc agtacgcgct gtttgtggaa gatgaatggc ggatcttcga gccgctggcg
    19321 ctgacgaccg gcgtgcgtat ggacgatcac gaaacctacg gtgaacactg gagtccgcgt
    19381 gcctacatgg tttataacgc caccgacacc gtaacggtga aagggggctg ggcgacggca
    19441 tttaaagcac cttctctgtt gcaacttagc cctgactgga cgagcaattc ctgccgtggc
    19501 gcatgtaaga ttgtgggtag cccggatctg aaaccagaaa ccagcgaaag ttgggagctg
    19561 gggctttact acatgggtga agaaggctgg ctggaagggg gtgaatccag cgttatcgtt
    19621 ttccgtaacg atgtgaaaga tcgtatcagc atcagccgta cgtctgacgt caacgctgca
    19681 ccgggctacc aaaactttgt tggttttgag acgggcgcta acggacggcg cataccggta
    19741 tttagctact acagcgttaa caaagctcgt attcagggcg tggaaaccga actgaaaatt
    19801 ccgttcaacg atgaatggaa actgtcgatc aactacacct acaacgatgg tcgtgatgtc
    19861 agcaacggcg aaaacaaacc gctatccgat ctgccgttcc atactgctaa cggtacgctg
    19921 gactggaaac cgctggcgct ggaagactgg tcattctatg tttctggtca ctataccggg
    19981 cagaaacgcg ccgacagcgc gacggctaaa acaccgggcg gttataccat ctggaatacc
    20041 ggcgcggcct ggcaggtgac taaagacgtc aaactgcgcg caggcgtgct gaaccttggc
    20101 gacaaggatc tcagtcgtga cgactacagc tataacgaag acggacgtcg ttactttatg
    20161 gcagtggatt atcgcttctg atgagaagat gcccggcgaa ccgggcggac tttcacttca
    20221 gtaaatactg cgcatggaag cgcaggtgat cctctataaa agaggcgatg aagtagtaac
    20281 tgtgatcata tcccggctga atacgcagcg tcatcggcca cgctttctgg cgcgcggctt
    20341 ctgccaatac cgcaggttgc aactggtcgg caagaaactg atcattatcg ccctgatcga
    20401 taagcgtcgg gatcgcatcc tgcgcgttac tggcatacat cagcgcgcaa ctatcccatt
    20461 ccagcatgca tttttgtcct cacctaaata ggtgctaaac gctttgattc cccacgggac
    20521 gctgcacgga ttcacaatgg gcgcaaaggc cgaaacgctg gtgtatttac ccggattttt
    20581 cagcgccata atcagcgcac cgtgaccacc cattgagtga ccgctaatgg cgcagcggtc
    20641 gctgacatta aattgcgact gaaccagcgc cggtaattca tcgcgcagat aatcatacat
    20701 ccggtaatgc gtcgcccacg gcggttgcgt ggcattaaga taaaagcctg cgccctggcc
    20761 taaatcgtag ccatcgtcgt tggcaacctt ttcgccgcgc gggctggtgt ctggcatcac
    20821 cagtacaatc cccagttccg ccgctacccg ctgggcaccc gccttggtgg tgaagttctc
    20881 gtcattgcag gttaatccgg aaagccagta cagcactggt ggcggggtgt gatcacgagg
    20941 gggggggaga aagatactga acgtcatcgg gcagtttaag gtactggagt cgtgtcgcca
    21001 gcgttgctgc cagccttcaa aacagcggtg ctcttcgagc atttccatgc gtggctcctt
    21061 gttgtgttgt ttgcaatttc ttcatcatac cgtttattca atgtgctgtg agtaactttc
    21121 acttccgtat ttgcataacg atgttttaac atctgctgat gaaaggcagc ggcaattaca
    21181 ataattatcg ctgtgaatac tggattatgt gcgccgcctc acgcacaata atcaagctgt
    21241 aaatcagctt aataactttg cccccacgca gggtggaggc gtcacacctg caggagaaat
    21301 cataaatgcc atcactcagt aaagaagcgg ccctggttca tgaagcgtta gttgcgagag
    21361 gactggaaac accgctgcgc ccgcccgtgc atgaaatgga taacgaaacg cgcaaaagcc
    21421 ttattgctgg tcatatgacc gaaatcatgc agctgctgaa tctcgacctg gctgatgaca
    21481 gtttgatgga aacgccgcat cgcatcgcta aaatgtatgt cgatgaaatt ttctccggtc
    21541 tggattacgc caatttcccg aaaatcaccc tcattgaaaa caaaatgaag gtcgatgaaa
    21601 tggtcaccgt gcgcgatatc actctgacca gtacctgtga acaccatttt gttaccatcg
    21661 atggcaaagc gacggtggct tatatcccga aagattcggt gatcggtctg tcaaaaatta
    21721 accgtatcgt gcagttcttt gcccagcgtc cgcaggtgca ggaacgtctg acgcagcaaa
    21781 ttcttattgc gctacaaacg ctgctgggca ccaataacgt ggctgtctcg atcgacgcgg
    21841 tgcattactg cgtgaaggcg cgtggcatcc gcgatgcaac cagtgccacg acaacgacct
    21901 ctcttggtgg attgttcaaa tccagtcaga atacgcgcca cgagtttctg cgcgctgtgc
    21961 gccatcacaa ctgattaaaa ggcaggaacc atggagcgca acgtcacgct cgattttgtt
    22021 cgcggcgtcg ccattctggg gatcctgcta ttaaacatca gcgcctttgg gctaccaaag
    22081 gcggcttatc tcaatcccgc ctggtacgat gctattacgc cgcaggatgc atggacctgg
    22141 gcatttctcg atctcatcgg ccaggtgaaa ttcctcacgc tttttgcgct gctgtttggt
    22201 gctggcctgc aaatgttgct gccccgtggc agacgctgga tccagtcgcg gttaacgctg
    22261 ttagtcttgt tgggctttat tcacggttta ttgttctggg acggcgatat tctgctggct
    22321 tacgggctgg tggggcttgg cgttttgctg ttattggggc tgatttccga cagccagacc
    22381 agccgcgcct ggacgccgga tgcatcggct attttgtatg aaaaatactg gaagcttcac
    22441 ggcggcgttg aagcgatcag taatcgtgcc gatggtgttg gcaacagttt actggcactg
    22501 ggcgcacagt atggctggca actggcaggg atgatgctca ttggtgcggc gctgatgcgc
    22561 agcggctggc tgaaagggca gttcagctta cgtcactatc gtcgtactgg ttttgtgctg
    22621 gtggcgattg gggtgaccat taaccttcct gccatcgccc tgcaatggca gctggactgg
    22681 gcgtatcgct ggtgtgcctt cttacttcaa atgccgcggg aactgagtgc gccgtttcag
    22741 gcgattggct atgcgtcgct gttttatggc ttctggccgc aattgagccg ctttaagctg
    22801 gtgcttgcga tcgcctgcgt cggacggatg gcgctgacca actatctatt gcaaacgctg
    22861 atttgtacca cgctttttta ccacctcggt ttgtttatgc attttgaccg cctggagctg
    22921 ctggcgtttg ttattccggt atggctggcg aatatcctct tctctgttat ctggctgcgt
    22981 tacttccgcc aggggccggt ggaatggctc tggcgtcagt taactttgcg tgctgccgga
    23041 ccggcaatat ctaaaacatc aagataacga tctggattac attcgttaac aaaacggctg
    23101 taaccgtttc cattgctgtg actcgattca cgaagtcctg tattcagtgc tgacaaaata
    23161 gccgccagca agcagtcatt tactgcaatc tcataacagg tagtgaatat gatcaccatt
    23221 cgtgatgtag cgcgtcaggc tggcgtctct gtggcaacgg tttcccgggt gctcaataac
    23281 agcacgctgg tcagtgccga cacgcgtgaa gcagtaatga aagccgtgag tgagctggat
    23341 tatcggccaa acgccaatgc ccaggcgctg gcaactcagg ttagcgacac cattggcgtg
    23401 gtggtgatgg acgtttctga tgcgtttttc ggcgcgctgg taaaagcggt ggatctggtc
    23461 gcccagcagc atcagaaata cgtgctaatc ggcaatagct atcatgaagc ggaaaaagag
    23521 cgtcacgcca ttgaggtgtt aattcgccag cgttgtaatg cgttgattgt tcactcaaaa
    23581 gcattgagtg acgatgaact ggcgcaattt atggataaca ttcccggtat ggtgttaatc
    23641 aaccgcgttg tgccggggta cgcccatcgt tgcgtttgcc tggataatct cagcggtgcc
    23701 cgaatggcga cgcgcatgtt gctgaataac ggtcatcaac gtattggtta tctttcttcc
    23761 agccacggca ttgaagatga cgccatgcgt aaagcaggct ggatgagtgc gttgaaagag
    23821 caggatatta ttccgccgga aagctggatt ggcactggta cgccggacat gccgggcggt
    23881 gaggcggcga tggttgaact gctggggcgc aatctacaac ttaccgctgt atttgcttat
    23941 aacgacaata tggccgctgg cgcactgaca gcattaaaag ataatggcat tgcgattccg
    24001 ttacatctct caatcatcgg tttcgatgat attcccatcg cccgttacac cgacccgcaa
    24061 ttaacgaccg tgcgttatcc cattgcttca atggctaaat tagccaccga actggccttg
    24121 cagggggcag caggcaatat tgatcctcgt gccagccact gttttatgcc gacgttagtg
    24181 cgtcgccatt ctgtcgcaac gcgccagaat gcggcggcga tcactaactc aacaaatcag
    24241 gcgatgtaac cgctttcaat ctgtgagtga tttcacagta tcttaacaat gtgatagcta
    24301 tgattgcacc gttttaatgt tgtaacccgt atgtaacagt gaataatcac tttcaccgag
    24361 gtaatagcgt cataacaaca attaaagccg ttttctggag cgttaccggg catggaagaa
    24421 cgaattttaa aaagtgagct tcggcgttca gtaacacttc attaactcta ctgccccgcc
    24481 gagcatttat ctcaagcact accctgcata agaaaaaccg gagataccat gaataagaag
    24541 gtgttaaccc tgtctgctgt gatggccagc atgttattcg gtgccgctgc acacgctgct
    24601 gatactcgca ttggtgtaac aatctataag tacgacgata actttatgtc tgtagtgcgc
    24661 aaggctattg agcaagatgc gaaagccgcg ccagatgttc agctgctgat gaatgattct
    24721 cagaatgacc agtccaagca gaacgatcag atcgacgtat tgctggcgaa aggggtgaag
    24781 gcactggcaa tcaacctggt tgacccggca gctgcgggta cggtgattga gaaagcgcgt
    24841 gggcaaaacg tgccggtggt tttcttcaac aaagaaccgt ctcgtaaggc gctggatagc
    24901 tacgacaaag cctactacgt tggcactgac tccaaagagt ccggcattat tcagggcgat
    24961 ttgattgcta aacactgggc ggcgaatcag ggttgggatc tgaacaaaga cggtcagatt
    25021 cagttcgtac tgctgaaagg tgaaccgggc catccggatg cagaagcacg taccacttac
    25081 gtgattaaag aattgaacga taaaggcatc aaaactgaac agttacagtt agataccgca
    25141 atgtgggaca ccgctcaggc gaaagataag atggacgcct ggctgtctgg cccgaacgcc
    25201 aacaaaatcg aagtggttat cgccaacaac gatgcgatgg caatgggcgc ggtagaagca
    25261 ctgaaagcac acaacaagtc cagcattccg gtgtttggcg tcgatgcgct gccagaagcg
    25321 ctggcgctgg tgaaatccgg tgcactggcg ggcacagtac tgaacgatgc taacaaccag
    25381 gcgaaagcga cctttgatct ggcgaaaaac ctggccgatg gcaaaggtgc ggctgatggc
    25441 accaactgga aaatcgacaa caaagtggtc cgcgtacctt atgttggcgt agataaagac
    25501 aacttggctg aattcagcaa gaaataagac tgattgggcg cagtcgttta ctgcgccctt
    25561 attgtatggc caacaaggta aaattatatg gtcagctcaa cgactccgtc ttccggggaa
    25621 tacttgttgg aaatgagcgg tatcaacaag tcctttcctg gtgttaaggc acttgataac
    25681 gttaatttaa aagtccggcc acattctatc catgcattaa tgggggaaaa cggtgcagga
    25741 aaatcgacat tattaaaatg cctgtttggt atttatcaaa aagactccgg caccatttta
    25801 ttccagggta aagagatcga tttccattcc gccaaagagg cactggaaaa tggtatttcg
    25861 atggtacacc aggagttaaa cctggtatta caacgttcgg tgatggataa catgtggctg
    25921 gggcgatatc ccaccaaagg catgtttgtc gatcaggaca aaatgtaccg cgaaaccaaa
    25981 gcgatttttg atgaactgga tattgatatc gatccgcgtg cgcgcgtcgg cacattatcc
    26041 gtttcgcaaa tgcagatgat cgaaatcgcc aaagcgtttt cctataacgc gaaaattgtg
    26101 attatggatg aaccgacttc ttcgttaacc gaaaaagagg tcaatcatct gttcactatt
    26161 attcgtaaat taaaagagcg cggctgcggt attgtttata tctcgcataa aatggaagaa
    26221 atcttccagt tatgtgatga agttaccgta ttgcgcgacg gtcagtggat cgccaccgaa
    26281 ccgctggcag gactgacgat ggacaagatc atcgccatga tggttgggcg ttctcttaat
    26341 cagcgttttc ctgacaaaga aaacaagccg ggcgaagtca tcctcgaggt gcgtaaccta
    26401 acgtcactgc gccagccgtc gattcgcgat gtctcgtttg atctgcataa aggggagatc
    26461 ctcggtattg ccgggctggt gggggcgaaa cgtaccgata ttgttgagac gttatttggt
    26521 attcgcgaga aatcggctgg caccattacg ttgcacggca aacagatcaa taaccataat
    26581 gccaacgaag ccataaacca cggatttgca ctggtaactg aggagcgccg ctcaacggga
    26641 atttatgcct atctggatat tggttttaac tcgttaattt ccaatattcg caactacaaa
    26701 aataaagttg gtttactgga taactcgcgg atgaaaagcg atacccagtg ggtgattgat
    26761 tcgatgcggg taaaaacgcc gggtcatcgg acgcaaattg gttcgctctc cggtggtaat
    26821 cagcaaaagg tgattattgg tcgctggcta ctaacgcaac cagaaatatt aatgctcgat
    26881 gaaccgacgc gcggtattga tgtcggggcg aagtttgaaa tttatcagtt aattgccgaa
    26941 ctggcgaaga aaggcaaggg gattattatt atctcctctg aaatgcctga gttgttaggg
    27001 ataacagacc gtattctggt catgagcaat ggtctcgttt ccggaattgt cgatacaaaa
    27061 acaacaacgc aaaacgaaat tctgcgtctt gcgtctttgc acctttaaga ttaggggctt
    27121 cccatgagtg cgttaaataa gaaaagtttt cttacttacc tgaaagaggg cggtatttac
    27181 gtcgttcttt tagttttgct ggcgattatt attttccagg acccaacatt tttaagtctg
    27241 ttgaacttaa gtaatattct cacccagtca tcggtgcgta ttattatcgc gctcggtgtg
    27301 gcagggttaa ttgtcaccca ggggaccgat ctttctgctg gtcgtcaggt agggctggcg
    27361 gcagtggtgg ctgcgacatt attgcagtcc atggataacg ccaacaaagt gttcccggaa
    27421 atggcgacga tgccgattgc gctggttatt ctgattgtct gtgccattgg tgcggtgatc
    27481 ggtttgatca acggtctgat tatcgcttat ctcaacgtga cgccgttcat taccacgctc
    27541 ggcacgatga tcatcgtcta tggcatcaac tcgctctatt acgactttgt cggggcgtcg
    27601 ccaatttctg gttttgacag tggcttctct acctttgctc agggctttgt cgcgctgggg
    27661 agtttccgtc tctcttacat caccttctac gcgttgattg cggtggcgtt cgtctgggtg
    27721 ttgtggaaca aaacccgctt cggtaagaac atttttgcca ttggcggtaa cccggaagcg
    27781 gcaaaaatat ctggtgtcaa cgtcggcctg aacctgctga tgatctacgc gttgtctggc
    27841 gtgttctatg cctttggcgg gatgttagaa gccggacgta tcggctctgc caccaacaac
    27901 ctcggcttta tgtatgagct ggatgctatc gcggcgtgcg tggtaggcgg tgtatcgttc
    27961 agcggcggtg tggggacggt gattggcgtg gtgaccgggg taattatttt taccgtcatc
    28021 aactatggcc tgacgtatat cggcgtaaac ccatactggc agtacatcat caaaggggcg
    28081 attattatct tcgccgtagc gctggattca ctgaaatacg cgcgtaagaa gtgatgtgat
    28141 tgcggatatt gccggatgcg tctctggttt tacaacgtta ccgggtgttc tttctcgcct
    28201 ttcttaaact tcacttcccc gagctcaata caacccaccg ggcagacgtg accacaaagc
    28261 agacaaccca cacatttctc ggtattacaa tgcggtgtgc gggttttctc gctccattcc
    28321 atcgcctggt gaccgccgtc gtaacaggaa atataacagc gtccacagcc aacacatttg
    28381 tcaagattga tacggggata gacaatataa ctgcggtcta aatcttccgc cgggacaata
    28441 ttgttattcg ccagacctac catctcctgc aacgaatcaa atccctgatc ggcgagataa
    28501 tgcgataacc cgctcgccat atcttctacg atgcgatagc cgtactgcat aatgccggtg
    28561 gtgacctgta acgttgctgc gccgagcaat aaaaactccg ccgcatcttc ccaggtttca
    28621 atgccgccga taccgctgat tgggaaatcg cgcagttctg gatgggtgcg catttgctga
    28681 atgaagcgca gggcgatcgg ttttaccgct ttaccggaat atccggaaat actcgattta
    28741 ccgttaacga tcggcatacc gattttctga ttaagatcga tattggtgat ggatttaacg
    28801 gtgttaattg ccgcaatgcc atctgcgccg ccgcgcttcg ccgccagcgc cacttcgcac
    28861 atatcaccga tattcggcgt catcttcgcc agcattggca gcgtggaacc ccgtttcact
    28921 gcccgacaat atttttctac cagctccggg ctttgcccga catcgctacc catcgcatga
    28981 gaagtcattt gcggacagga gaagttacac tcgatcatat ccgcgccagc tgctcccatt
    29041 gctgctcatt ttcccccatg atcgaagcga tcaatacttt gtccgggtaa tcttccttca
    29101 gccgacgcag ggcggtcaga ttctcttcca acggatgttc agcaatctgc tccatatttt
    29161 tgaagccgat aaaaccggta tcttctttca ccagatgatc aaaacgcggc gagacttcgt
    29221 tggcgataaa aaagccgatc gttttaaaca ccacaccgcc ccaacctgtg tcgtaggctt
    29281 tggcacacat ctcatagcag ttgcctaccg gcgaagaaga gaggcagaac gggttgggaa
    29341 agttcacgcc gcaaaaagta atcgaaagat ctttcgttaa catgagcaag ctccctctaa
    29401 atagtgatga atcgcctcgg cggcttcttt cccggttttc acggcataga ccacggtttt
    29461 gtcaccctca acaatatcgc cagcagcaaa gacttgcgga tcgcggatct gataattttg
    29521 tgttttgatg gtgttacgct gctgctctaa ctccgcaaag gcatccagtc tggcatgctg
    29581 accgacggcg agaataattt tatctgccgc catcgtcagt tcgcctgata accgtacatg
    29641 cttaaacgtg actttattgc cttccacggc tactggcgtg aatccatcga tgatcgaaac
    29701 gcccagctcc cgggcgctgg caaactcttt ttcgcttgcc ggaaactcat ctaactcttc
    29761 acgtgctacg caagttaccg cctgacagcc gagaactttc agcgtgctgg ctacgtccat
    29821 cgcgacatca ccgccgccga taattaatgc gctttgtgga atgctgatat cgccttgcgc
    29881 ctgtcgtaca cgttgcagga agtcgacggc aatctcaacg tcgctatgct caaacagcgg
    29941 tagcccggaa ccgcttgata accccacggt gaccagtacc gcgcggtttt ccgctttcag
    30001 ttgctccagg gtgagtgttt taccgatttc gttattgcac ttaatggtca cgcccatttt
    30061 ttcaatacgg gcgatctctg catccagcac tgattgcggt aaccggaatt gcggaatacc
    30121 gttacgcaac cagccaccgg ggtgcgcttc tttctcataa atcgtgacgt cataaccctg
    30181 gtttgtcagt gtcacactgg cctgcaatcc ggcaggacct gcgccaataa tcgcgacttt
    30241 gccgagcgtt ttagtaccgg gctgataaat ttccatcccg gtttgttgtt caaaatcagt
    30301 aacaaaacgc tgtaagcggc cgatatcaat gggagcatca acaccggcac gggtacaacc
    30361 gctttggcat aatttctccg tcgggcacac tctggcacaa acggcaccga gggcattatt
    30421 ttcgcgaatt gtctcggcag cgcctttaaa attacgaaag tagattgagc gaataaattt
    30481 ccccggatcg gtctgtgccg ggcaagcctg actacaggga gcgtcgtgac ataataaaca
    30541 gcgagaggct tctttaatcg ccagtaacgg cgtaaaagcc ggagtgagtt catccagata
    30601 attttgttgc ggcatgtgat cttccttaat gctatgagta attatggcga acgctaaata
    30661 aatattgcgt gctgatatta aggctatgcg cgtggggaaa tttaagctgt gaaaggattc
    30721 acaaatagct gcggttcaga atgttatatc cgcagcttat tgagtgaagt
//
LOCUS       DAZPCS010000032        30582 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZPCS010000032 DAZPCS010000000
VERSION     DAZPCS010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30582
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.032"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..588
                     /locus_tag="ACHUQ7_001447"
     CDS             <1..588
                     /locus_tag="ACHUQ7_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HFR1134923.1"
                     /translation="IPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEV
                     EIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKP
                     HTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKM
                     VVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            818..1201
                     /gene="secE"
                     /locus_tag="ACHUQ7_001448"
     CDS             818..1201
                     /gene="secE"
                     /locus_tag="ACHUQ7_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFR1134924.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLAAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATIAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            1203..1748
                     /gene="nusG"
                     /locus_tag="ACHUQ7_001449"
     CDS             1203..1748
                     /gene="nusG"
                     /locus_tag="ACHUQ7_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFR1134925.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1907..2335
                     /gene="rplK"
                     /locus_tag="ACHUQ7_001450"
     CDS             1907..2335
                     /gene="rplK"
                     /locus_tag="ACHUQ7_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFR1134926.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            2339..3043
                     /gene="rplA"
                     /locus_tag="ACHUQ7_001451"
     CDS             2339..3043
                     /gene="rplA"
                     /locus_tag="ACHUQ7_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFR1134927.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3456..3953
                     /gene="rplJ"
                     /locus_tag="ACHUQ7_001452"
     CDS             3456..3953
                     /gene="rplJ"
                     /locus_tag="ACHUQ7_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFR1134928.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            4020..4385
                     /gene="rplL"
                     /locus_tag="ACHUQ7_001453"
     CDS             4020..4385
                     /gene="rplL"
                     /locus_tag="ACHUQ7_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFR1134929.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4705..8733
                     /gene="rpoB"
                     /locus_tag="ACHUQ7_001454"
     CDS             4705..8733
                     /gene="rpoB"
                     /locus_tag="ACHUQ7_001454"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFR1134930.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8810..13033
                     /gene="rpoC"
                     /locus_tag="ACHUQ7_001455"
     CDS             8810..13033
                     /gene="rpoC"
                     /locus_tag="ACHUQ7_001455"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFR1134931.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(13275..14408)
                     /gene="thiH"
                     /locus_tag="ACHUQ7_001456"
     CDS             complement(13275..14408)
                     /gene="thiH"
                     /locus_tag="ACHUQ7_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFR1134932.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAVLTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14405..15175)
                     /gene="thiG"
                     /locus_tag="ACHUQ7_001457"
     CDS             complement(14405..15175)
                     /gene="thiG"
                     /locus_tag="ACHUQ7_001457"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFR1134933.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVALLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHATQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEVGLLARQSGPGSRSYFAHATSPLTGFLEASA"
     gene            complement(15177..15377)
                     /gene="thiS"
                     /locus_tag="ACHUQ7_001458"
     CDS             complement(15177..15377)
                     /gene="thiS"
                     /locus_tag="ACHUQ7_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFR1134934.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(15361..16116)
                     /gene="thiF"
                     /locus_tag="ACHUQ7_001459"
     CDS             complement(15361..16116)
                     /gene="thiF"
                     /locus_tag="ACHUQ7_001459"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFR1134935.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(16109..16744)
                     /gene="thiE"
                     /locus_tag="ACHUQ7_001460"
     CDS             complement(16109..16744)
                     /gene="thiE"
                     /locus_tag="ACHUQ7_001460"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFR1134936.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16744..18639)
                     /gene="thiC"
                     /locus_tag="ACHUQ7_001461"
     CDS             complement(16744..18639)
                     /gene="thiC"
                     /locus_tag="ACHUQ7_001461"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFR1134937.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(18720..18820)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18873..19349)
                     /gene="rsd"
                     /locus_tag="ACHUQ7_001462"
     CDS             complement(18873..19349)
                     /gene="rsd"
                     /locus_tag="ACHUQ7_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFR1134938.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19444..20217
                     /gene="nudC"
                     /locus_tag="ACHUQ7_001463"
     CDS             19444..20217
                     /gene="nudC"
                     /locus_tag="ACHUQ7_001463"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFR1134939.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSLLLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20257..21321
                     /gene="hemE"
                     /locus_tag="ACHUQ7_001464"
     CDS             20257..21321
                     /gene="hemE"
                     /locus_tag="ACHUQ7_001464"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFR1134940.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRHELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21478..22149
                     /gene="nfi"
                     /locus_tag="ACHUQ7_001465"
     CDS             21478..22149
                     /gene="nfi"
                     /locus_tag="ACHUQ7_001465"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709792.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFR1134941.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSKPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22192..22782
                     /locus_tag="ACHUQ7_001466"
     CDS             22192..22782
                     /locus_tag="ACHUQ7_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFR1134942.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMGDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            22969..23241
                     /gene="hupA"
                     /locus_tag="ACHUQ7_001467"
     CDS             22969..23241
                     /gene="hupA"
                     /locus_tag="ACHUQ7_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFR1134943.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23254..23949
                     /locus_tag="ACHUQ7_001468"
     CDS             23254..23949
                     /locus_tag="ACHUQ7_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFR1134944.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23951..24376)
                     /gene="zraP"
                     /locus_tag="ACHUQ7_001469"
     CDS             complement(23951..24376)
                     /gene="zraP"
                     /locus_tag="ACHUQ7_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFR1134945.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24614..25990
                     /gene="zraS"
                     /locus_tag="ACHUQ7_001470"
     CDS             24614..25990
                     /gene="zraS"
                     /locus_tag="ACHUQ7_001470"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFR1134946.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMYR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            25987..27312
                     /gene="zraR"
                     /locus_tag="ACHUQ7_001471"
     CDS             25987..27312
                     /gene="zraR"
                     /locus_tag="ACHUQ7_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFR1134947.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGAQD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27309..28598)
                     /gene="purD"
                     /locus_tag="ACHUQ7_001472"
     CDS             complement(27309..28598)
                     /gene="purD"
                     /locus_tag="ACHUQ7_001472"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFR1134948.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFAAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28610..30199)
                     /gene="purH"
                     /locus_tag="ACHUQ7_001473"
     CDS             complement(28610..30199)
                     /gene="purH"
                     /locus_tag="ACHUQ7_001473"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFR1134949.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7163 a         7962 c         8418 g         7039 t
ORIGIN      
        1 attccggaac cagagcgtgc gattgacaag ccgttcctgc tgccgatcga agacgtattc
       61 tccatctccg gtcgtggtac cgttgttacc ggtcgtgtag aacgcggtat catcaaagtt
      121 ggtgaagaag ttgaaatcgt tggtatcaaa gagactcaga agtctacctg tactggcgtt
      181 gaaatgttcc gcaaactgct ggacgaaggg cgtgctggtg agaacgtagg tgttctgctg
      241 cgtggtatca aacgtgaaga aatcgaacgt ggtcaggtac tggctaagcc gggcaccatc
      301 aagccgcaca ccaagttcga atctgaagtg tacattctgt ccaaagatga aggcggtcgt
      361 catactccgt tcttcaaagg ctaccgtccg cagttctact tccgtactac tgacgtgact
      421 ggtaccatcg aactgccgga aggcgtagag atggtaatgc cgggcgacaa catcaaaatg
      481 gttgttaccc tgatccaccc gatcgcgatg gacgacggtc tgcgtttcgc aatccgtgaa
      541 ggcggccgta ccgttggcgc gggcgttgta gcaaaagttc tgagctaatt gccgataaca
      601 tttgacgcaa tgcgcactaa aagggcatca tttgatgccc tttttgcacg ctttcgtacc
      661 agaacctggc tcatcagtga ttttctttgt cataatcatt gctgagacag gctctgttga
      721 gggcgtataa tccgaaaagc taatacgcgt ttcgatttgg tttgcctcgc gatcgcgggg
      781 tgaaaatgtt tgtagaaaac ttctgacagg ttggtttatg agtgcgaata ccgaagctca
      841 aggaagcggg cgcggcctgg aagcgatgaa gtgggtcgtt gtggtggcat tgctcctggc
      901 ggcgattgtc ggcaactacc tttatcgcga cattatgctg ccgctgcgtg cgctggccgt
      961 agtaattctg attgctgcag cgggtggtgt cgcgctgtta acgacaaaag gtaaagctac
     1021 cattgctttt gcccgtgaag cgcgtaccga agtccgtaag gtcatttggc cgactcgcca
     1081 ggaaacattg cacaccacgc tgattgtggc tgcggttacc gcagtaatgt cactgatcct
     1141 gtggggactg gatggtattc tggttcgcct ggtatccttt atcactggcc tgaggttctg
     1201 agatgtctga agctcctaaa aagcgctggt acgtcgttca ggcgttttcc ggttttgaag
     1261 gccgcgtagc aacgtcgctg cgtgagcata tcaaattaca caacatggaa gatttgtttg
     1321 gtgaagtcat ggtaccaacc gaagaagtgg ttgaaatccg tggcggtcag cgtcgcaaaa
     1381 gcgaacgtaa attcttccct ggctacgtcc tcgttcagat ggtgatgaac gacgcgagct
     1441 ggcacttggt gcgcagcgta ccgcgtgtga tgggcttcat cggcggtact tccgatcgtc
     1501 ctgcgccaat cagcgataaa gaagtcgatg cgattatgaa ccgcctgcag caggttggtg
     1561 ataagccgcg tccgaaaacg ctgtttgaac cgggtgaaat ggtccgtgtt aatgatggtc
     1621 cgttcgctga cttcaacggt gttgttgaag aagtggatta cgagaaatct cgtctgaaag
     1681 tgtctgtttc tatcttcggt cgtgcgaccc cggtagagct ggacttcagc caggttgaaa
     1741 aagcctaacc cagcgatcaa aaaagcggcg atttaatcgt tgcacaaggc gtgagattgg
     1801 aatacaattt cgcgcctttt gtttttatgg gccttgcccg taaaacgatt ttttatatca
     1861 cggggagcct ctcagaggcg ttattaccca acttgaggaa tttataatgg ctaagaaagt
     1921 acaagcctat gtcaagctgc aggttgcagc tggtatggct aacccgagtc cgccagtagg
     1981 tccggctctg ggtcagcagg gcgtaaacat catggaattc tgcaaagcgt tcaacgcaaa
     2041 aactgattcc atcgaaaaag gtctgccgat tccggtagta atcaccgttt acgctgaccg
     2101 ttctttcact ttcgttacca agaccccgcc ggcagcagtt ctgctgaaaa aagcggcagg
     2161 tatcaagtct ggttccggta agccgaacaa agacaaagtg ggtaaaattt cccgcgctca
     2221 gctgcaggaa atcgcgcaga ccaaagctgc cgacatgact ggtgccgaca ttgaagcgat
     2281 gactcgctcc atcgaaggta ctgcacgttc catgggcctg gtagtggagg actaagaaat
     2341 ggctaaactg accaagcgca tgcgtgttat ccgtgagaaa gttgatgcaa ccaaacagta
     2401 cgacatcaac gaagctatcg cactgctgaa agagctggcg actgctaaat tcgtagaaag
     2461 cgtggacgta gctgttaacc tcggcatcga cgctcgtaaa tctgaccaga acgtacgtgg
     2521 tgcaactgta ctgccgcacg gtactggccg ttccgttcgc gtagccgtat ttacccaagg
     2581 tgcaaacgct gaagctgcta aagctgcagg cgcagaactg gtaggtatgg aagatctggc
     2641 tgaccagatc aagaaaggcg aaatgaactt tgacgttgtt attgcttctc cggatgcaat
     2701 gcgcgttgtt ggccagctgg gccaggttct gggtccgcgt ggcctgatgc caaacccgaa
     2761 agtgggtact gtaacaccga acgttgctga agcggttaaa aacgctaaag ctggccaggt
     2821 tcgttaccgt aacgacaaaa acggcatcat ccacaccacc atcggtaaag tggactttga
     2881 cgctgacaaa ctgaaagaaa acctggaagc tctgctggtt gcgctgaaaa aagcaaaacc
     2941 gactcaggcg aaaggcgtgt acatcaagaa agttagcatc tccaccacca tgggtgcagg
     3001 tgttgcagtt gaccaggctg gcctgagcgc ttctgtaaac taatgccttt acgtgggcgg
     3061 tgattttgtc tacaatctta cccccacgta taatgcttaa tgcagacgta tatccgagat
     3121 attcgggttg tggcaaggcg gcaactgagt gagtcgccaa gagcatagct aactatgtga
     3181 ctggtgcgaa tgaaggaagc caacgccgcc acaagttgaa tagcgacgga tagaaaagat
     3241 ttgttcgttg gagcctggcc tatccaggcc tccgtcgaag accgcaggag tttcgcaaga
     3301 aacttaatcc cctgcgtaga cggtgacaga acgctaagat tattctttta tattctggct
     3361 tgtttctgct caccgtaatt aagacgctct ctccgtttgg aggagtgaag tgagttccag
     3421 agattttctc tggcaaacat ccaggagcaa agctaatggc tttaaatctt caagacaaac
     3481 aagcgattgt tgctgaagtc agcgaagtag ccaaaggcgc gctgtctgca gtagttgcgg
     3541 attcccgtgg cgtaactgta gataaaatga ctgaactgcg taaagcaggt cgcgaagctg
     3601 gcgtatacat gcgtgttgtt cgtaacaccc tgctgcgccg tgctgttgaa ggtactccgt
     3661 tcgagtgcct gaaagacgcg tttgttggtc cgaccctgat tgcatactct atggaacacc
     3721 cgggcgctgc tgctcgtctg ttcaaagagt tcgcgaaagc gaatgcaaaa tttgaggtca
     3781 aagccgctgc ctttgaaggt gagctgatcc cggcgtctca gatcgaccgc ctggcaactc
     3841 tgccgaccta cgaagaagca attgcacgcc tgatggcaac catgaaagaa gcttcggctg
     3901 gcaaactggt tcgtactctg gctgctgtac gcgatgcgaa agaagctgct taatcgcagt
     3961 tatcttttta acgcattcgc ttacgtataa acttattctg atattcagga acaatttaaa
     4021 tgtctatcac taaagatcaa atcattgaag cagttgcagc tatgtctgta atggacgttg
     4081 tagaactgat ctctgcaatg gaagaaaaat tcggtgtttc cgctgctgct gctgtagctg
     4141 tagctgctgg cccggttgaa gctgctgaag aaaaaactga attcgacgta attctgaaag
     4201 ctgctggcgc taacaaagtt gctgttatca aagcagtacg tggcgcaact ggcctgggtc
     4261 tgaaagaagc taaagacctg gtagaatctg caccggctgc tctgaaagaa ggcgtgagca
     4321 aagacgacgc agaagcactg aaaaaagctc tggaagaagc tggcgctgaa gttgaagtta
     4381 aataagccaa cccttccggt tgcagcctga gaaatcaggc tgatggctgg tgacttttta
     4441 gtcaccagcc tttttgcgct gtaaggcgcc agtagcgttt cacactgttt gactactgct
     4501 gtgcctttca atgcttgttt ctatcgacga cttaatatac tgcgacagga cgtccgttct
     4561 gtgtaaatcg caatgaaatg gtttaagcgt gatagcaaca ggcattgcgg aaagtgttcc
     4621 attttccggt caacaaaata gtgttgcaca aactgtccgc tcaatggaca gatgggtcga
     4681 cttgtcagcg agctgaggaa ccctatggtt tactcctata ccgagaaaaa acgtattcgt
     4741 aaggattttg gtaaacgtcc acaagttctg gatgtacctt atctcctttc tatccagctt
     4801 gactcgtttc agaaatttat cgagcaagat cctgaagggc agtatggtct ggaagctgct
     4861 ttccgttccg tattcccgat tcagagctac agcggtaatt ccgagctgca atacgtcagc
     4921 taccgccttg gcgaaccggt gtttgacgtc caggaatgtc aaatccgtgg cgtgacctat
     4981 tccgcaccgc tgcgcgttaa actgcgtctg gtgatctatg agcgcgaagc gccggaaggc
     5041 accgtaaaag acattaaaga acaagaagtc tacatgggcg aaattccgct catgacagac
     5101 aacggtacct ttgttatcaa cggtactgag cgtgttatcg tttcccagct gcaccgtagt
     5161 ccgggcgtct tctttgactc cgacaaaggt aaaacccact cttcgggtaa agtgctgtat
     5221 aacgcgcgca tcatccctta ccgtggttcc tggctggact tcgaattcga tccgaaggac
     5281 aacctgttcg tacgtatcga ccgtcgccgt aaactgcctg cgaccatcat tctgcgcgcc
     5341 ctgaactaca ccacagagca gatcctcgac ctgttctttg aaaaagttat ctttgaaatc
     5401 cgtgataaca agctgcagat ggaactggtg ccggaacgcc tgcgtggtga aaccgcatct
     5461 tttgacatcg aagctaacgg taaagtgtac gtagaaaaag gccgccgtat cactgcgcgc
     5521 cacattcgcc agctggaaaa agacgacgtc aaactgattg aagtcccggt tgagtacatc
     5581 gcaggtaaag tggttgctaa agactatatt gatgagtcta ccggcgagct gatctgcgca
     5641 gcgaacatgg agctgagcct ggatctgctg gctaagctga gccagtctgg tcacaagcgt
     5701 atcgaaacgc tgttcaccaa cgatctggat cacggcccat atatctctga aaccttacgt
     5761 gtcgacccaa ctaacgaccg tctgagcgca ctggtagaaa tctaccgcat gatgcgccct
     5821 ggcgagccgc cgactcgtga agcagctgaa agcctgttcg agaacctgtt cttctccgaa
     5881 gaccgttatg acttgtctgc ggttggtcgt atgaagttca accgttctct gctgcgcgaa
     5941 gaaatcgaag gttccggtat tctgagcaaa gacgacatca ttgatgttat gaaaaagctc
     6001 atcgatatcc gtaacggtaa aggcgaagtc gatgatatcg accacctcgg caaccgtcgt
     6061 atccgttccg ttggcgaaat ggcggaaaac cagttccgcg ttggcctggt acgtgtagag
     6121 cgtgcggtga aagagcgtct gtctctgggc gatctggata ccctgatgcc tcaggatatg
     6181 atcaacgcca agccgatttc cgcagcagtg aaagagttct tcggttccag ccagctgtct
     6241 cagtttatgg accagaacaa cccgctgtct gagattacgc acaaacgtcg tatctccgca
     6301 ctcggcccag gcggtctgac ccgtgaacgt gcaggcttcg aagttcgaga cgtacacccg
     6361 actcactacg gtcgcgtatg tccaatcgaa acccctgaag gtccgaacat cggtctgatc
     6421 aactctctgt ccgtgtacgc acagactaac gaatacggct tccttgagac tccgtatcgt
     6481 aaagtgaccg acggtgttgt aactgacgaa attcactacc tgtctgctat cgaagaaggc
     6541 aactacgtta tcgcccaggc gaactccaac ctggatgaag aaggccactt cgtagaagac
     6601 ctggtaactt gccgtagcaa aggcgaatcc agcttgttca gccgcgacca ggttgactac
     6661 atggacgtat ccacccagca ggtggtatcc gtcggtgcgt ccctgatccc gttcctggaa
     6721 cacgatgacg ccaaccgtgc attgatgggt gcgaatatgc aacgtcaggc cgttccgact
     6781 ctgcgcgctg ataagccgct ggttggtact ggtatggaac gtgctgttgc cgttgactcc
     6841 ggtgtaactg cggtagctaa acgtggtggt gtcgttcagt acgtggatgc ttcccgtatc
     6901 gttatcaaag ttaacgaaga cgagatgtat ccgggtgaag caggtatcga catctacaac
     6961 ctgaccaaat acacccgttc taaccagaac acctgtatca accagatgcc gtgtgtgtct
     7021 ctgggtgaac cggttgaacg tggcgacgtg ctggcagacg gtccgtccac cgacctcggt
     7081 gaactggcgc ttggtcagaa catgcgcgta gcgttcatgc cgtggaatgg ttacaacttc
     7141 gaagactcca tcctcgtatc cgagcgtgtt gttcaggaag accgtttcac caccatccac
     7201 attcaggaac tggcgtgtgt gtcccgtgac accaagctgg gtccggaaga gatcaccgct
     7261 gacatcccga acgtgggtga agctgcgctc tccaaactgg atgaatccgg tatcgtttac
     7321 attggtgcgg aagtgaccgg tggcgacatt ctggttggta aggtaacgcc gaaaggtgaa
     7381 actcagctga ccccagaaga aaaactgctg cgtgcgatct tcggtgagaa agcctctgac
     7441 gttaaagact cttctctgcg cgtaccaaac ggtgtatccg gtacggttat cgacgttcag
     7501 gtctttactc gcgatggcgt agaaaaagac aaacgtgcgc tggaaatcga agaaatgcag
     7561 ctcaaacagg cgaagaaaga cctgtctgaa gaactgcaga tcctcgaagc gggtctgttc
     7621 agccgtatcc gtgctgtgct ggtagccggt ggcgttgaag ctgagaagct cgacaaactg
     7681 ccgcgcgatc gctggctgga gctgggcctg acagacgaag agaaacaaaa tcagctggaa
     7741 cagctggctg agcagtatga cgaactgaaa cacgagttcg agaagaaact cgaagcgaaa
     7801 cgccgcaaaa tcacccaggg cgacgatctg gcaccgggcg tgctgaagat tgttaaggta
     7861 tatctggcgg ttaaacgccg tatccagcct ggtgacaaga tggcaggtcg tcacggtaac
     7921 aagggtgtaa tttctaagat caacccgatc gaagatatgc cttacgatga aaacggtacg
     7981 ccggtagaca tcgtactgaa cccgctgggc gtaccgtctc gtatgaacat cggtcagatc
     8041 ctcgaaaccc acctgggtat ggctgcgaaa ggtatcggcg acaagatcaa cgccatgctg
     8101 aaacagcagc aagaagtcgc gaaactgcgc gaattcatcc agcgtgcgta cgatctgggc
     8161 gctgacgttc gtcagaaagt tgacctgagt accttcagcg atgaagaagt tatgcgtctg
     8221 gctgaaaacc tgcgcaaagg tatgccaatc gcaacgccgg tgttcgacgg tgcgaaagaa
     8281 gcagaaatta aagagctgct gaaacttggc gacctgccga cttccggtca gatccgcctg
     8341 tacgatggtc gcactggtga acagttcgag cgtccggtaa ccgttggtta catgtacatg
     8401 ctgaaactga accacctggt cgacgacaag atgcacgcgc gttccaccgg ttcttacagc
     8461 ctggttactc agcagccgct gggtggtaag gcacagttcg gtggtcagcg tttcggggag
     8521 atggaagtgt gggcgctgga agcatacggc gcagcataca ccctgcagga aatgctcact
     8581 gttaagtctg atgacgtgaa cggtcgtacc aagatgtata aaaacattgt ggacggcaac
     8641 catcagatgg agccgggcat gccagaatcc ttcaacgtat tgttgaaaga gattcgttcg
     8701 ctgggtatca acatcgaact ggaagacgag taattctcgc tcaaacaggt cactgctgtc
     8761 ggggtaaaac ccggcagcgg attgtgctaa ctccgacggg agcaaatccg tgaaagattt
     8821 attaaagttt ctgaaagcgc agactaaaac cgaagagttt gatgcgatca aaattgctct
     8881 ggcttcgcca gacatgatcc gttcatggtc tttcggtgaa gttaaaaagc cggaaaccat
     8941 caactaccgt acgttcaaac cagaacgtga cggccttttc tgcgcccgta tctttgggcc
     9001 ggtaaaagat tacgagtgcc tgtgcggtaa gtacaagcgc ctgaaacacc gtggcgtcat
     9061 ctgtgagaag tgcggcgttg aagtgaccca gactaaagta cgccgtgagc gtatgggcca
     9121 catcgaactg gcttccccga ctgcgcacat ctggttcctg aaatcgctgc cgtcccgtat
     9181 cggtctgctg ctcgatatgc cgctgcgcga tatcgaacgc gtactgtact ttgaatccta
     9241 tgtggttatc gaaggcggta tgaccaacct ggaacgtcag cagatcctga ctgaagagca
     9301 gtatctggac gcgctggaag agttcggtga cgaattcgac gcgaagatgg gtgcggaagc
     9361 aatccaggct ctgctgaaga gcatggatct ggagcaagag tgcgaacagc tgcgtgaaga
     9421 gctgaacgaa accaactccg aaaccaagcg taaaaagctg accaagcgta tcaaactgct
     9481 ggaagcgttc gttcagtctg gtaacaaacc agagtggatg atcctgaccg ttctgccggt
     9541 actgccgcca gatctgcgtc cgctggttcc gctggatggt ggtcgtttcg cgacttctga
     9601 cctgaacgat ctgtatcgtc gcgtcattaa ccgtaacaac cgtctgaaac gtctgctgga
     9661 tctggctgcg ccggacatca tcgtacgtaa cgaaaaacgt atgctgcagg aagcggtaga
     9721 cgccctgctg gataacggtc gtcgcggtcg tgcgatcacc ggttctaaca agcgtcctct
     9781 gaaatctttg gccgacatga tcaaaggtaa acagggtcgt ttccgtcaga acctgctcgg
     9841 taagcgtgtt gactactccg gtcgttctgt aatcaccgta ggtccatacc tgcgtctgca
     9901 tcagtgcggt ctgccgaaga aaatggcgct ggagctgttc aaaccgttca tctacggcaa
     9961 gctggaactg cgtggtcttg ctaccaccat taaagctgcg aagaaaatgg ttgagcgcga
    10021 agaagctgtc gtttgggata tcctggacga agttatccgc gaacacccgg tactgctgaa
    10081 ccgtgcaccg actctgcacc gtctgggtat ccaggcattt gaaccggtac tgatcgaagg
    10141 taaagctatc cagctgcacc cgctggtttg tgcggcatat aacgccgact tcgatggtga
    10201 ccagatggct gttcacgtac cgctgacgct ggaagcccag ctggaagcgc gtgcgctgat
    10261 gatgtctacc aacaacatcc tgtccccggc gaacggcgaa ccaatcatcg ttccgtctca
    10321 ggacgttgta ctgggtctgt actacatgac ccgtgactgt gttaacgcca aaggcgaagg
    10381 catggtgctg actggcccga aagaagcaga acgtctgtat cgctctggtc tggcttctct
    10441 gcatgcgcgc gttaaagtgc gtatcaccga gtatgaaaaa gatgctaacg gtgaattagt
    10501 agcgaaaacc agcctgaaag acacgactgt tggccgtgcc attctgtgga tgattgtacc
    10561 gaaaggtctg ccttactcca tcgtcaacca ggcgctgggt aaaaaagcaa tctccaaaat
    10621 gctgaacacc tgctaccgca ttctcggtct gaaaccgacc gttatttttg cggaccagat
    10681 catgtacacc ggcttcgcct atgcagcgcg ttctggtgca tctgttggca tcgatgacat
    10741 ggtcatcccg gagaagaaac acgaaatcat ctccgaggca gaagcagaag ttgctgaaat
    10801 tcaggagcag ttccagtctg gtctggtaac tgcgggcgaa cgctacaaca aagttatcga
    10861 tatctgggct gcggcgaacg atcgtgtatc caaagcgatg atggataacc tgcaaactga
    10921 aaccgttatt aaccgtgacg gtcaggaaga gaagcaggtt tccttcaaca gcatctacat
    10981 gatggccgac tccggtgcgc gtggttctgc ggcacagatt cgtcagcttg ctggtatgcg
    11041 tggtctgatg gcaaagccgg atggctccat catcgaaacg ccaatcaccg cgaacttccg
    11101 tgaaggtctg aacgtactcc agtacttcat ctccacccac ggtgctcgta aaggtctggc
    11161 ggataccgca ctgaaaactg cgaactccgg ttacctgact cgtcgtctgg ttgacgtggc
    11221 gcaggacctg gtggttaccg aagacgattg tggtacccat gaaggtatca tgatgactcc
    11281 ggttatcgag gggggtgacg ttaaagagcc gctgcgcgat cgcgtactgg gtcgtgtaac
    11341 tgctgaagac gttctgaagc cgggtactgc tgatattctc gttccgcgca acacgctgct
    11401 gcacgaacag tggtgtgacc tgctggaaga gaactctgtc gacgcggtaa aagtacgttc
    11461 tgttgtatct tgtgacaccg actttggtgt atgtgcgcac tgctacggtc gtgacctggc
    11521 gcgtggccac atcatcaaca aaggtgaagc aatcggtgtt atcgcggcac agtccatcgg
    11581 tgaaccgggt acacagctga ccatgcgtac gttccacatc ggtggtgcgg catctcgtgc
    11641 ggctgctgaa tccagcatcc aggtgaaaaa caaaggtagc atcaagctca gcaacgtgaa
    11701 gtcggttgtg aactccagcg gtaaactggt tatcacttcc cgtaacaccg aactgaaact
    11761 gatcgacgaa ttcggtcgta ccaaagaaag ctacaaagtg ccttacggtg cggtactggc
    11821 gaaaggcgat ggcgaacagg ttgctggcgg cgaaaccgtt gcaaactggg acccgcacac
    11881 catgccggtt atcaccgaag taagcggttt tgtacgcttt actgacatga tcgacggcca
    11941 gaccattact cgtcagaccg acgaactgac cggtctgtct tcgctggtgg ttctggattc
    12001 cgcagaacgt accgcaggtg gtaaagatct gcgtccggca ctgaaaatcg ttgatgctca
    12061 gggtaacgac gttctgatcc caggtaccga tatgcctgcg cagtacttcc tgccgggtaa
    12121 agcgattgtt cagctggaag atggcgtaca gatcagctct ggtgacacct tggcgcgtat
    12181 tccgcaggaa tccggcggta ccaaggacat caccggtggt ctgccgcgcg ttgcggacct
    12241 gttcgaagct cgtcgtccga aagagccggc aatcctggct gaaatcagcg gtatcgtttc
    12301 cttcggtaaa gaaaccaaag gtaaacgtcg tctggttatc accccggtag acggtagcga
    12361 tccgtacgaa gagatgattc cgaaatggcg tcagctcaac gtgttcgaag gtgaacgtgt
    12421 agaacgtggt gacgtaattt ccgacggtcc ggaagcgccg cacgacattc tgcgtctgcg
    12481 tggtgttcat gctgtgactc gttacatcgt taacgaagta caggacgtat accgtctgca
    12541 gggcgtaaag attaacgata aacacatcga agttatcgtt cgtcagatgc tgcgtaaagc
    12601 taccatcgtt aacgcgggca gctccgactt cctggaaggc gaacaggttg aatactctcg
    12661 cgtcaagatc gcaaaccgcg aactggaagc gaacggcaaa gtgggtgcaa cttactcccg
    12721 cgatctgctg ggtatcacca aagcgtctct ggcaaccgag tccttcatct ccgcggcatc
    12781 gttccaggag accactcgcg tgctgaccga agcagccgtt gcgggcaaac gcgacgaact
    12841 gcgcggcctg aaagagaacg ttatcgtggg tcgtctgatc ccggcaggta ccggttacgc
    12901 gtaccaccag gatcgtatgc gtcgccgtgc tgcgggtgaa gctccggctg caccgcaggt
    12961 gactgcagaa gacgcatctg ccagcctggc agaactgctg aacgcaggtc tgggcggttc
    13021 tgataacgag taatcgttaa tccgcaaata acgtaaaaac ccgcttcggc gggttttttt
    13081 atgggtgtta ttcagagggc aaattcttca cggaaaatat taagaaaggc agggcgaaac
    13141 gaggaagaag cttttcgccc tggacaacat tcctgctgac ggcactacca taaaaaaaca
    13201 caacgctttc aacaaccttg cgtttttcat cttccgtagc ggctcggaat aaatttacgt
    13261 tcttacattc cgtctcatag tctttgcgag gcgcgtccca gatagctgtc ccagtctttc
    13321 cataccggct gcaaaccctg agcggttaac acggcagcaa ccgcttccgg tctgcgatcg
    13381 tcgtgcggtg agaactgttc caactcgggg tgattatcgg catagccacc tggctgcgtt
    13441 ttcgagaagg cgctgacgtt attgatcgcc agcggaataa cgcgatcgcg aaaccacggt
    13501 gattcccgcg tggagagtga cagttcaatc tccggtgcaa gcagtcggaa ggcgcagatg
    13561 gtttgcacta actggcgttc atccataatc gacgcaggct caatgccgcc agtacacggg
    13621 cgcaggcgcg gaaaggagac agagtaacgg ctttgccagt aatgctgttg cagccatagc
    13681 aaatgttcgg caaccatata gcagtcaacg cgccagttgt cggaaaggcc aattagcgcg
    13741 ccgaggccta tcttatcaat ccccgcacgc cccagccgat ccggcgtttc cagccgccag
    13801 aagaagtcct gttttttgcc tttcaaatga tggcgggtat aagtcgcctc gtgatatgtc
    13861 tcctgataaa ccatcacccc atccagacca agttgcttta actcggcgta ttccgtctcc
    13921 gccagcggtt gcacttccat ttgtagtgaa gagaactgtt cacgcagggc ggggagatga
    13981 cgacgaaagt aatccatccc cactttcgcc tgatgttcac cagtgactaa tagcagatgt
    14041 tcaaagccca tctcccgtat agcggcgctt tccctggcaa tatccgcttc atccagcgtt
    14101 ttgcgcttga tgcgattact catggaaaat ccgcagtacg tgcagtcgtt agcgcaaaga
    14161 ttggaaagat aaagcgggac gtagaaacta actgtgttgc caaatcgctg acgggtcaga
    14221 cgctgcgcct gttgggccag ttgttccaga tagccactgg cggcaggcga taacagcgcc
    14281 atcatgtcgt cgcgggtgag ttgcgaggca tttagcgccc gctctacgtc agcagccgtt
    14341 ttgccgttga tacgcaggcg gatgtcgtcc cagtccagtt gtcgccagcg attgctgaag
    14401 gttttcatgc cgatgcctcc agaaatccgg tcagcgggct ggtggcatga gcaaaataac
    14461 tgcggctgcc cggtccggac tgacgtgcca gcaggcctac ttctaccgcc agacgaaatg
    14521 ccttcgccat gttgacggga tcgtccgcga cggcaatcgc cgtattcact aacaccgcgt
    14581 cggcccccat ttccagcgcc tgcgtggcat ggctgggaac gccgatgcca gcatcgacaa
    14641 ccaccggaac tgtggcctgc tggataataa tctccagcat ggcacgggtt tccagtccct
    14701 gattcgagcc aatcggcgcg ccgagcggca tcaccgctgc acagccgact tcttccagac
    14761 gtttacacaa taccggatcg gccccgcagt aaggcagcac gacaaatccc tgttgtacca
    14821 gcgtttcggc ggctttcagg gtttcgatgg gatcgggcaa cagccagcgg gcgtcagggt
    14881 gaatctccaa ttttagccag tttgtgccta acgcttcacg tgccagatgg gcggcgaaaa
    14941 tggcttcttc cgctgttttc gccccggaag tattcggcag cagggccaca cccgccgcga
    15001 taagcggttc gaggatagcg tcgttgtgct ggcgcaagtc gacacgtttc atcgccagtg
    15061 tcaccagctg gctgccggaa gcgcggatcg cctccaccat cagttgtgaa gaagcgaatt
    15121 tccctgtgcc ggtaaacaga tgtgaatcaa acgttttgtc cgcaatacgt aacatctcaa
    15181 ccccctgcaa taacctgaaa aagcaggatc tggtcgccat cctgcacgat atgttgcgtc
    15241 cactgctcac gcgggacgat ttgctgatta atcgccagag ccgcgcccgc ttgtcgttgg
    15301 tcgagttgct ccagtagttc gtgaacagtt tgcccggcgg cgcactgcat cggttgatcg
    15361 ttaaacagga tctgcattgc ttcctccgca taccgggcaa ccactggcgc ggcgcaacgc
    15421 caggctgcgc cactggctcg acttaccgtc gaacagtcgg agttctcccg caggcgtctc
    15481 tataccgctt aataacttaa tggcttccag tgcctgcaaa gtgcccatca ccccgaccac
    15541 cgggccaacc acgcccgccg tgcggcagtt gcgttctggc tgctggttat ctggccacag
    15601 gcagcggtaa cacccctgct cccagggcgg tgtcagtacc atcaactgac cgccaaatcc
    15661 gaccgcgctg gcggtgataa gcggcgtgtt gagtgccacg caggcggcat taatctcctg
    15721 gcgagtcgcc atattgtcgg tacagtcgag caccacatcg gcttgtgcaa ccgcatcttt
    15781 caacgcctca cccgttaacc gttgttgtaa tgctgtcagt tgaatgtcgg gattcaactg
    15841 tgtcagtcgc tgttggctga cctgcgattt cgggctgttg atatcttcag tggtaaagag
    15901 gatttgtcgt tgcagattgc ttaaatgcac atcgtcgtca tctgccagta ccagcgtccc
    15961 gacgccagcc cctgccaggt atagcgcagc aggtgtaccc agcccgccaa gaccaataat
    16021 cagcacctgg ctgtcgagca gtttttgctg cccgtcaaga gcgatatcgt cgagcagaat
    16081 ttggcggcta taacgcataa agtcacggtc attcatcgcc aactcctgca atttccagca
    16141 actgtgccgt tgccaaacgc cagtctgcgg cttgagtaat ggcgctgacg acggcgatac
    16201 tgccgacacc cgttgctatc accgcaggcg cgcgtgccag actgataccg ccaatcgcca
    16261 cggtgggata atccgccagg cgctcaacat gccgtgccaa ctgttccagc ccctgcggtg
    16321 cagaaggcat ctgtttggtt tgcgtcggga acacatgtcc cagcgcgata taagaggggc
    16381 gtgctgccag cgcgacgtcg atttccatat cgtcatgtgt cgaaacgccc agccgcaggc
    16441 ctgccgcgcg gatggcattg agatcggtgg cttgcaaatc ttcctgcccc aaatggacgc
    16501 catacgcctg atgcttgatc gcgagccgcc agtaatcgtt gataaacaat cgcgcgttat
    16561 agcggcggcc cagcgcaatt gccgccacga catcggcttc cacctcttca tcgcgccgat
    16621 ctttgatgcg tagctggaga gtacgtacgc ctgcatccaa cagacgttcg atccactgta
    16681 cgctgtccac caccgggtac agtcctaaac gaaaaggtac aggaggaaaa tcaggctgat
    16741 acatcacgct tcctccttat gcaggtagat ttctccgcct ctggcacgga agttctccga
    16801 catatccgcc attcccactt caatggtttg cgcggcggcg taatcgcgta cttcctggct
    16861 gattttcatc gagcagaatt ttggcccaca catggagcaa aaatgggcga ctttgccgga
    16921 ctcttggggc agggtttcat cgtgataagc gcgggcggta aacgggtcga gggccagatt
    16981 aaactggtct tcccagcgaa attcgaagcg ggctttcgac atggcgttat cgcgaatttg
    17041 cgcgcccgga tgcccttttg ccaggtcagc agcgtgggcg gcaatcttat aagtgataag
    17101 cccctgctta acatcttctt tattgggcag acccagatgc tcttttggcg ttacgtaaca
    17161 gagcatcgcg cagccaaacc agccaatcat cgccgcacca atccccgacg tgaagtggtc
    17221 atagcccggt gcaatatcgg tagttagcgg ccccagagtg taaaacggcg cttcgtggca
    17281 gtgctctaac tcctcggtca tattgcggcg gatcatctgc atcggcacat ggcctgggcc
    17341 ttcaatcatc acctgtacgt catattccca ggcgattttg gtcagttcgc ccagcgtatg
    17401 caattcggca aactgcgctt catcgttggc gtcctgaata gaaccggggc gcagaccgtc
    17461 gcccagcgac agcgaaacgt cataagcggc acagatttca caaatttcgc ggaagtgttg
    17521 atagaggaaa ttttcctgat gatgggagag gcaccatttc gccataatcg aaccgccgcg
    17581 agagacgata ccggtcaggc gtttcgcggt catcggcaca tagcgcagca gtacgcccgc
    17641 atggatagtg aagtaatcca caccttgctc ggcctgttcc agcagcgtgt cgcggaacgc
    17701 ttcccaggta agatcttcgg cgatcccgtt aaccttctcc agcgcctggt agatcggcac
    17761 tgtaccgatc ggcaccggac tgttacgcaa aatccactcg cgggtttcgt gaatatagcg
    17821 accggtggag agatccatca ccgtatccgc tccccagcgc gtggaccata ccagcttttc
    17881 cacttcttct tcgatggaag aggtgaccgc cgagttgccg atattggcgt taacttttac
    17941 caggaagtta cggccaataa tcatcggctc cgattccgga tgattaatgt tggccgggat
    18001 aatcgcacgt ccggcagcaa cttcatcacg gacaaattcc gcagtgatat tttccggcag
    18061 atgtgcgcca aagctcattc ccggatgctg gtggcgtaaa acctcgctac ggatgcgctc
    18121 gcggcccata ttctcgcgga tggcgatgaa ttccatttcc ggcgtgatga tgccctggcg
    18181 ggcgtagtgc agttgggtga cacggcgtcc tgttttggcg cgttttggcg ttaataagcc
    18241 gctaaaacgt agctcgtcga gaccatcatc tgccagccgc gttctggtgt aatcggaact
    18301 gcgcacggta agttcttcgg tatcggcgcg cgcatcgatc cacggctggc gtagttttgc
    18361 cagcccttgc tgcacgttaa tggcgatctg cggatcacca tacgggccgg aggtgtcgta
    18421 gaccggaatc gcttcgtttt cttcgtactg cggctgttct ttgctaccgc caattagcgt
    18481 cgggctaagc tggatctcac gcatcggcac gcgcacgccg gggtgtgtgc cagtgatata
    18541 aatgcgtttt gagttgggaa aggcggtgcc ttccagggtg tcgataaaat gttgggcccg
    18601 ggcgcgttgt tcgcggcggg tcagttttgt tgcagacata gctcattcca aaaagttaag
    18661 gacgtggctt gtcagacgac ggatgaagca agagacgatc gccgcaaagg cgatgcgata
    18721 gcagattaac tcttgttccc ttcgcaggta ttagcctgat caggttccgc ggatcccgaa
    18781 taaacggtct cagccagtta aggcactccg acaagaaatt agcccccaca aacggggcgt
    18841 tgaatgtaaa ttacgcgtta acagcgcaga actcaagcag gatgtttgac gcgggcggcg
    18901 tcaagcacca gcagaatcag cttatcttcc agcacaaagc gcgcttccag cgcctcgccg
    18961 atgtcagata aaacctgttg aaactcaagg taattatcat gatcgatggc ggtttccagg
    19021 ctggaatcgt agtaatccat aatctgttgg gtattggctt cgagttgcgg ccaaatcttc
    19081 gcggcgcgcg cgagttgccc gttgccttcc agcttatgaa gaatgcgctc ataaatactg
    19141 aaatgtccgg cagacaagta atcgaccagg ctctgacaaa aatcatcaag ggctttttcg
    19201 tttagcctca tgtacgattc tttgccaggc ttaatgccaa ccagattgta gtaagccacg
    19261 agcagatgct tacgtacatg tagccagcga tcaaccagtt tgttacttcc tctgacgcgc
    19321 tccgtcaggt tatcgagctg gttaagcatg attgactccg caagtttgta ttcaaaaact
    19381 gctcagtgag aaatgtaaaa accatgttaa acatgccagt gatgcaaagg tagtgcaaga
    19441 gctatggatc gtataattga aaaattagat cacggctggt gggtcgtcag ccatgaacaa
    19501 aaattatggt tgccgaaggg agaattgcca tatggcgaag cggcaaattt cgatcttgtg
    19561 ggtcagcgcg cactacaaat cggcgaatgg cagggggaac ctgtttggtt aatacaacag
    19621 cagcggcgtc acgatatggg gtcggtacgt caggtcattg atctcgatgt tgggctgttt
    19681 caactggccg gacgtggcgt gcaactggcg gagttttacc gatcgcataa atactgtggt
    19741 tactgcgggc atgaaatgta tccgagcaaa accgaatggg cgatgctgtg tagccattgc
    19801 cgtgagcgtt actacccgca aatcgccccc tgcattattg ttgccatccg tcgcgatgat
    19861 tcgctcctcc tcgcccagca tacccgccat cgtaacggtg tccatacagt acttgccgga
    19921 ttcgtcgaag tgggcgaaac cctcgagcag gcagtcgcgc gggaagtgat ggaagagagc
    19981 ggaattaaag ttaaaaactt gcgttacgtg acttctcagc cgtggccgtt tcctcagtct
    20041 ttaatgaccg cgtttatggc ggaatatgac agcggcgaca tcgtgatcga cccgaaagaa
    20101 ttgctcgagg cgaactggta tcgctatgac gatttgccgt tactcccgcc gcccggcacc
    20161 gtagcgcgcc gtctgataga agatactgtg gcgatgtgtc gggcagagta tgagtgatga
    20221 tacactgacc gcctgacgca ctaaggaaca gccaaaatga ccgaacttaa aaacgatcgt
    20281 tatctgcggg cgctgctgcg ccagcccgtt gatgtcactc cagtatggat gatgcgccag
    20341 gcgggtcgct atctaccgga atataaagcc acgcgcgccc aggcgggcga ttttatgtcg
    20401 ctgtgcaaaa acgccgagct ggcgtgcgaa gtgactttgc aaccgctgcg tcgctacccg
    20461 ctggatgcgg cgatcctctt ttccgatatc ctcaccgtgc cggacgcgat ggggttaggg
    20521 ctctattttg aagccggaga aggtccgcgt tttacctcgc cagtcacctg caaagccgac
    20581 gtcgataaac tgccaattcc ggacccggaa gatgagctgg gttacgtgat gaacgcggtg
    20641 cgtaccattc gtcacgaact gaaaggcgaa gtgccgctga ttggtttttc cggcagcccg
    20701 tggacgctgg caacctacat ggtggaaggc ggcagcagca aagccttcac cgtgatcaaa
    20761 aaaatgatgt atgccgatcc gcaggcgctg cacgctctgc tcgataaact ggcgaaaagc
    20821 gtcactttgt atctgaatgc gcagattaaa gccggtgctc aggcagtgat gattttcgac
    20881 acctggggcg gcgtgcttac cgggcgcgat tatcaacagt tctcgctcta ttacatgcat
    20941 aaaattgttg atggtttact gcgtgaaaac gacggtcgcc gcgtgccagt cacgctgttt
    21001 accaaaggcg gcggacagtg gctggaagcc atggctgaaa ccggttgcga tgcgctgggc
    21061 ctcgactgga caacggacat cgccgatgcg cgccgccgtg tgggcaataa agtcgcgttg
    21121 cagggtaata tggatccgtc gatgctgtac gcgccgcctg cccgcattga agaagaagta
    21181 gcgactatac ttgcaggttt cggtcacggc gaaggtcatg tctttaacct tggtcacggc
    21241 attcatcagg atgtgccgcc agaacatgct ggcgtgttcg tggaggcagt gcatcgactg
    21301 tctgaacagt atcaccgcta agagcctgat ccatcaggct attttatttg ccattttgag
    21361 gccgggcagt gctcaaaatc ctcacgtact acgtgtacgc tccggttttt ccgcgctgtc
    21421 cggcctcaaa ctgccttcaa caattacgcc tgttgaacca ggctctaagg agcgattatg
    21481 gatctcgcgt cattacgcgc tcaacaaatc gaactggctt cttctgtgat ctgcgaggat
    21541 cgactcgata aagatccacc ggatctgatc gccggagccg atgtcgggtt tgagcagggc
    21601 ggagaagtga cgcgagcggc gatggtgctg ctgaaatatc cctcgcttga gctggtggag
    21661 tataaagttg cccgcatcgc caccaccatg ccttacattc caggttttct ttccttccgc
    21721 gaatatcctg cgctgctggc agcgtgggag atgctgtcgc aaaagccgga tttagtgttt
    21781 gtcgatggtc atgggatctc gcatcctcgc cgtcttggcg tcgccagcca ttttggctta
    21841 ttggtggatg tgccgaccat tggcgtggcg aaaaaacggc tctgcggtaa attcgaaccg
    21901 ctctccagca aaccgggcgc gctggcccca ctgatggata aaggcgagca gctggcctgg
    21961 gtctggcgca gcaaagcgcg ctgtaacccg ttgtttatcg ctaccggcca tcgggtcagc
    22021 gtggacagcg cgctggcgtg ggtacaacgc tgcatgaaag gctatcgtct gccggagcca
    22081 acgcgctggg cggacgcggt ggcctcggaa cgtccggcgt tcgtgcgcta tacagcaaat
    22141 cagccctaat tcaggtaaac tgcggataat ttccgtattt gagaactcaa catgttacaa
    22201 aacccaattc atctgcgtct ggagcgcctg gaaagctggc agcatgtcac tttcatggct
    22261 tgcttatgcg aacgcatgta ccccaattac gccatgttct gccagcaaac cggttttggt
    22321 gatgggcaaa tttaccgtcg tattctcgat ctcatctggg aaacgctgac cgttaaagac
    22381 gcaaaagtaa atttcgacag ccaactggag aaatttgaag aagcgattcc ttcagccgac
    22441 gatttcgatc tgtacggcgt ttatccggca atcgatgcct gcgtggcgtt aagtgaactg
    22501 gtccattcgc gtttgagtgg tgaaacgctc gaacacgcgg tggaagtgag taagacctcc
    22561 atcacgaccg ttgcgatgct ggaaatgact caggctggtc gcgaaatggg cgatgaagag
    22621 cttaaagaaa acccagctgt agagcaagaa tgggacattc agtgggaaat attccgactt
    22681 ttagctgagt gcgaagaacg cgatatcgag ctgataaaag gccttagggc agacctgcgt
    22741 gagtcgggtg agagcaatat tggtataatt tttcagcaat aagaccagaa aacgtgattt
    22801 aacgcctgat ttgtcgtacc tggagtcttc cctttcgccc cccgtctggt ctacatttgg
    22861 ggggcgaaaa aaagtggcta tcggtgcgtg tatgcaggag agtgctattc tggcatttcc
    22921 gtcgcactcg atgcttagca agcgataaac acattgtaag gataacttat gaacaagact
    22981 caactgattg atgtaattgc agagaaagca gaactgtcca aaacccaggc taaagctgct
    23041 ctggagtcca ctctggctgc aattactgag tctctgaaag aaggcgatgc tgtacaactg
    23101 gttggtttcg gtaccttcaa agtgaaccat cgcgctgagc gtactggccg caacccgcag
    23161 accggtaaag aaatcaaaat tgccgcagct aacgtaccgg catttgtttc tggcaaggca
    23221 ctgaaagacg cagttaagta agattgcgtg gcagtgaaca gttttaacga aggggtggtt
    23281 tcaccccttt tgtctttctg gcgtcgatca ttgatgctgg caggtgcgct gtttctcact
    23341 gcctgtagcc acaactcttc acttcctccc tttaccgcca gtggatttgc tgaagaccag
    23401 ggcgcggtac gcatctggcg aaaagacagc ggcgataatg tgcatctgct tgccgtgttt
    23461 agcccgtggc gcagtggcga taccacgacg cgagagtatc gctggcaggg cgataacctc
    23521 acgctcatca atatcaatgt ttacagcaaa ccgccggtga acattcgcgc gcgttttgac
    23581 gatcgcggcg atctgagctt tatgcaacgt gaatccgatg gggaaaagca gcagctttct
    23641 aacgaccaaa tcgatttata ccgttatcgt gctgatcaga tccgccagat tagcgatgcc
    23701 ttacgtcagg ggagagtcgt gctgcgccag gggcgctggc atgcgatgga acagaccgtg
    23761 accacctgcg aagggcaaac cattaaacct gatttagatt cgcaggcgat agcgcatatc
    23821 gagcgccgcc agagccgctc ttctgttgat gtcagcgtgg catggctgga agcgcccgaa
    23881 ggttcgcaat tactgttagt ggcaaactct gatttctgtc gctggcaacc caacgagaaa
    23941 acgttctgat ttaccagtgg cccataccca tatgaccgcc accaccgcag ccgccgtagc
    24001 ccatgcccat tccggctccg cgcggaatac ccgcttcagc catcgcgata tctcgtttca
    24061 cccgtaactc atctaacgac tgacgcaaat tctccatctc tttggcgacc gcgttaattt
    24121 tgctgctatc cggtgggttc gcggctaaca gggcattgta ttcataacgc ttcgtcacca
    24181 gttgctgttg cagtgcgctg ctttgagcgt aaaagtcatt atggattttc tgccacgctg
    24241 tctgttgttc gctggtcaaa ggcgcggcat tttgctgcca cataccgtgt ccgccgtgag
    24301 caaatgcaga tgtcgatccc atcgccattg ctgaaagcgc catcattacc agggcaattt
    24361 tcgtgttccg tttcatggtt aatcctccag tggttgtctt acttcgggta ttgcatcttc
    24421 cgtgccaacg atgaaacgct gatatgacgg gtaatctggc atgataaacg agtaaaaatg
    24481 actcgcctgc tgcgggtagc gagtcatttt tactcattga aacttaagcc tttgtgttac
    24541 agcgcagggt aagcgctgat aaaagatggc atgatttctg ctgtcagaaa gggatgagca
    24601 ggcaaagaag aagatgcgtt ttatgcaacg ttctaaagac tccttagcta aatggttaag
    24661 cgcgatcctc cccgtggtca ttgttgggct ggtagggttg tttgcggtga cggtgattcg
    24721 cgattatggg cgcgagactg ccgccgccag acaaacgctg ctggaaaaag gcagtgtact
    24781 tattcgcgct cttgaatccg gctcgcgcgt cggcatgggg atgcgcatgc atcatgcgca
    24841 gcagcaggcc ttactggaag aaatggccgg gcagcctgga gtacgttggt ttgcggtcac
    24901 ggatgaacaa ggaacaatcg tgatgcatag caactccggc atggtgggaa aacagcttta
    24961 ttcccctcag gaaatgcagc agttacatcc gggagatgaa gaagcgtggc ggcggatcga
    25021 tagcgcagac ggcgagcctg ttctggaaat ttatcgccag tttcaaccga tgtttgctgc
    25081 tggaatgtac cggatgcgcc atatgcagca gtatgccgcg acaccacaag caattttcat
    25141 cgctttcgac gccagtaata ttgtgagtgc cgaagatcgt gagcagagaa acaccctgat
    25201 tattctcttc gccctggcga cggtcttgct ggcaagcgta ttgtcattct tctggtatcg
    25261 ccgctatctg cgctcgcgcc agcttctaca agatgaaatg aagcgcaaag agaagctggt
    25321 ggcgctgggg catcttgcgg caggcgttgc ccacgaaatc cgtaacccac tttcctcgat
    25381 taaaggactg gcgaaatact ttgccgagcg cgcgcctgca gggggagaag cgcatcaact
    25441 ggcgcaggtg atggcgaaag aggccgaccg tttaaaccgc gtggtaagcg agttgctgga
    25501 actggttaag ccaacgcatc tggctttgca ggcggtggat ctcaacacgc tgattaacca
    25561 ctcattacag ctggtaagcc aggatgcaaa cagccgggag atccagttac gctttaccgc
    25621 caacgacaca ttaccggaaa ttcaggccga tccggacagg ctgactcagg tcctgttgaa
    25681 tctctatctc aatgctattc aggcgattgg tcagcatggc gtgattagcg tgacggccag
    25741 cgaaagcggc gcgggcgtga aaatcagcgt taccgacagc ggtaagggaa ttgcggcaga
    25801 tcagcttgaa gccatcttca ctccgtactt caccaccaaa gccgaaggca ccggattggg
    25861 gctggcggtc gtgcataata ttgttgaaca acacggtggt acaattcagg tcgcaagcca
    25921 ggagggaaaa ggctcaacgt tcaccctctg gcttccggtc aatattacgc gtaaggaccc
    25981 acaaggatga cgcacgataa tatcgatatt ctggtggtgg atgatgacat tagccactgc
    26041 actattttgc aggctttact gcgcggctgg ggctataacg tcgcgctggc gaacagcggg
    26101 cgacaggcgc tggagcaggt gcgggaacag gtttttgatc ttgtgctttg cgatgtgcga
    26161 atggcggaga tggacggcat cgccacgctg aaagagatca aagcgttaaa cccggcaatt
    26221 ccggtgctga ttatgactgc gtactccagc gtcgagacgg cggtagaggc actgaaaact
    26281 ggggcgcagg attatctcat caagccgctg gatttcgata acctacaggc gacgctggaa
    26341 aaggcgctcg cgcatacgca cagtattgat gctgaaacac ctgcggtgac tgccagccag
    26401 ttcggtatgg tcggtaaaag cccggcgatg caacacctgc tcagtgaaat cgccctcgtc
    26461 gcgccatcgg aagccacggt gctgatccac ggcgattccg gcaccggtaa agagctggtc
    26521 gccagggcga ttcacgccag tagcgcacgt agcgaaaaac cgctggtaac gctcaactgt
    26581 gcggcactca acgaatcctt gctggaatct gaattgttcg gtcacgaaaa aggggcgttt
    26641 actggagccg ataaacgccg ggaagggcgc tttgttgagg cggacggcgg cacgctgttt
    26701 ctcgatgaaa ttggcgatat ctcgccgatg atgcaggtgc gtctgctgcg tgcgattcag
    26761 gagcgcgaag ttcagcgtgt cggtagcaac cagactatct cggttgatgt ccggctgatt
    26821 gcggcgaccc atcgcgatct tgccgcagag gtgaatgccg ggcgttttcg ccaggatctc
    26881 tactatcgcc tgaatgtggt ggcgattgaa gtaccatcgc tgcggcaacg gcgggaagat
    26941 atccctctgc tggctggcca ttttcttcag cgctttgccg agcgtaatcg caaggcggta
    27001 aaaggcttta cgccacaggc gatggatttg ctgatccact acgactggcc gggaaatatt
    27061 cgtgaactgg aaaacgcggt ggaacgggcg gtggtgctgc tgaccgggga atatatttcc
    27121 gaacgcgagc tgccgctggc gattgccagt acgccgatcc cgctggggca aagtcaggat
    27181 attcagccgc tggtggaagt ggaaaaagag gtgattctgg cggcgctgga gaaaacgggc
    27241 ggcaacaaaa ccgaagccgc ccgtcagtta gggatcacgc gcaaaacgct attggcaaaa
    27301 ctgtcgcgtt agttctgctc gcgttcgatg gcgcgccaac cgatatcttt ccggcagaag
    27361 caatcatccc agtggatatc ggtcattaag gcgtaggcgc gtttctgcgc ttctgccacg
    27421 gtatgaccca gcgcggtgac gcacagtacg cgcccgccgc tggttaccac ctgctcgtca
    27481 tccgccagtt ttgtgcccgc gtggaacact ttgccgtcag ccacttcttc cagcggcagg
    27541 ccgtggatca catcgccggt gcggtaatcg cccggatatc cacccgcagc catcaccacg
    27601 ctgagagaag cacgttcatc ccactctgac gttttctcgt ccagtttgcc ttcacaggcc
    27661 gccaggcaga gctcaaccag atcggacttc atgcgcagca taatcggctg ggtttccgga
    27721 tcgccaaagc ggcagttaaa ttcaataacc ttcggattgc cctgtttgtc gatcatcagg
    27781 cccgcataga gaaaaccggt gtaggtgttg ccttccgccg ccatgccttt cacggttggc
    27841 cagatgatac gttccatggt gcgctgatga acttcatcgg ttactaccgg cgcgggggag
    27901 taagcgccca tcccgccggt gtttggtccg ttatctttat cgcctacgcg tttgtgatcc
    27961 tggctggtag ccatcggcag cacatgctcg ccgtccacca tcacgataaa gctcgcttct
    28021 tcgccatcga ggaactcttc gataacgatg cgatgacccg cgtcgccaaa agcgttgccc
    28081 gccagcatat cgtgaacagc cgcttccgct tcttccagcg tcatcgccac gataacgcct
    28141 ttcccggcag ccagaccgtc cgctttaatg acgattggcg cgcctttctc acgcagatac
    28201 accagcgcag gttctacctc ggtgaagttc tggtattccg ccgtagggat cttatggcgg
    28261 gccaggaaat ctttggtaaa cgcttttgag ccttccagtt gggccgcacc tgcggttggg
    28321 ccgaagattt tcagcccggc ggcgcggaag gtatcgacca cgcctttcac cagcggcgct
    28381 tccgggccga cgatggtcag atcaatcttt tcgttttgtg cgaaatccaa cagcgccggg
    28441 atatcggtca cgccgatagc aacgttttgc agcgcgggtt ccagcgcagt gcctgcatta
    28501 cccggagcag caaaaacagt ctcaaccagc ggcgactggg ccgctttcca ggccagcgcg
    28561 tgctcgcgcc cgccgttacc aatcactaat actttcatct attgctccat taatggcgga
    28621 agtggcgcat gtcggtgaag agcatcgcaa taccgtgctc gtcggcggcg gcaatcactt
    28681 cgtcatcacg gatggaaccg ccaggctgga ttacgcaggt cacgcccgca gcggcggcgg
    28741 catcaatacc gtcgcggaac gggaagaacg cgtcagaagc catcgaggaa cctttcactt
    28801 ccaggccttc atcggcagct ttaataccgg cgattttcgc ggagtacacg cggctcatct
    28861 ggcccgcgcc aataccgatg gtcatattgt ttttggcata gacgatagca ttggatttca
    28921 cgaatttcgc cactttccag cagaacagcg catcacgcag ttcctgttcg gtcggctgac
    28981 gttgggtgac gacgcgcagt tcttccgcac cgaccatccc caggtcacga tcctgaacca
    29041 gcagaccgcc gttcacgcgt ttgaaatcaa gacccggaac acgctcgccc cactgaccgc
    29101 aggtcagaac gcgtacgttc tgcttggcgg cggtgatttt cagggcttct tcgctggcgg
    29161 aaggcgcaat aatcacttcg acaaactgac gagaaatgat ggcctgtgcg gtttccgcat
    29221 ccagctcgcg gttaaaggca atgatgccgc cgaatgcgga ggttgggtcg gttttgtacg
    29281 cgcgatcgta agcatcaaga atggaattgc cgatagctac accgcaaggg ttggcgtgct
    29341 tcacaatcac acatgccggc tcggcgaact ctttcacgca ctccagcgcc gcatcggtat
    29401 cggcgatgtt gttataagag agggctttac cctgaacctg ggttgcggta gcaacggagg
    29461 cttctttcac attctcttct atatagaagg cagcctgctg gtggctgttc tcgccgtaac
    29521 gcatatcctg cttcttaatg aagttcaggt tcagcgtgcg tgggaagcga ccggcggctt
    29581 ctttgctttc accgtggtac gccggaacca tgctgccgaa gtagttggca atcatgctgt
    29641 cgtaggcggc agtgtgttcg aaggctttga tggcgaggtc gaaacgggtt gcaagcgtca
    29701 gcgatccttc gttgtcatcc atctctttaa taatggcgtc gtagtcgctg ctcttcacca
    29761 cgattgcgac atctttatgg ttcttagcgg cggagcgcac catggtcggg ccgccgatat
    29821 cgatgttctc aaccgcatct tccagcgagc agccttcacg agcaacagtc tgggcgaacg
    29881 gatacaggtt aacaaccacc atatcgatag gctggatctg atgttcttcc ataatggcat
    29941 cgtcctggcc gcgacggccc agaatgccac catgtacttt cggatgcagg gtcttcacgc
    30001 gtccatccat catctccggg aaaccggtgt aatcggaaac ttcggttacc ggcagacctt
    30061 tttctgctaa cagacgggca gtgccccctg tagacagcag ctccacaccg cgtgcggaaa
    30121 gtgcctgggc gaattcgacg ataccggctt tgtcagaaac actgagcaga gcgcggcgga
    30181 ctggacgacg ttgttgcatg gtaaatcccc tgggtttgac tattacagag agcgttagct
    30241 gaatttttcg cgaaaaactc agctaacgcc cctaacgggg catccttatt tttcgcccgc
    30301 attgtaacga aaacgtttgc gcaacgctcg cgaatttttc tctttcaatg gtgatcacaa
    30361 ttttgacagt ggttaccgtg ggaaaaatac agaaattaca ttgatgattg tggataactc
    30421 tgtgcgtaaa aaggtataaa gcggggtttt gctggggaat gcagcagtca gtcatttttc
    30481 tgcaattttt ctattgcggc ctgcggagaa ctccctataa tgcgccttca tcgacacggc
    30541 ggatgtgaat cacttcacac aaacagccgg ttcggttgaa ga
//
LOCUS       DAZPCS010000033        29702 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZPCS010000033 DAZPCS010000000
VERSION     DAZPCS010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29702
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.033"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..1224
                     /locus_tag="ACHUQ7_001474"
     CDS             <1..1224
                     /locus_tag="ACHUQ7_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFR1134950.1"
                     /translation="FELPEITDNELLVSVISDSVCLSTWKAALLGSEHKRVPDDLENH
                     PVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSGYSAGYSYEYFGGNATYMI
                     IPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYHTTQYVYEHRMGVKPGGNI
                     ALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQVQKLLPVDLAASKGIELVY
                     VNTKGMSDPIQMLRALTGDVGFDDIFVYAAVPAVVEMADELLAEDGCLNFFAGLTDKN
                     FKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQLQPSFMVTHIGGLDAVPET
                     VLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKELARLVEETHGIWNEQAEK
                     YLLAQFGVDIGEAAQ"
     gene            1221..2186
                     /gene="glpX"
                     /locus_tag="ACHUQ7_001475"
     CDS             1221..2186
                     /gene="glpX"
                     /locus_tag="ACHUQ7_001475"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFR1134951.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            2208..2717
                     /gene="fumE"
                     /locus_tag="ACHUQ7_001476"
     CDS             2208..2717
                     /gene="fumE"
                     /locus_tag="ACHUQ7_001476"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HFR1134952.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            2714..3427
                     /locus_tag="ACHUQ7_001477"
     CDS             2714..3427
                     /locus_tag="ACHUQ7_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFR1134953.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKLLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQALPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPAAALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRGLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(3399..4076)
                     /locus_tag="ACHUQ7_001478"
     CDS             complement(3399..4076)
                     /locus_tag="ACHUQ7_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR1134954.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHNAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(4070..4747)
                     /locus_tag="ACHUQ7_001479"
     CDS             complement(4070..4747)
                     /locus_tag="ACHUQ7_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR1134955.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVIIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(4735..5442)
                     /locus_tag="ACHUQ7_001480"
                     /pseudo
     CDS             complement(4735..5442)
                     /locus_tag="ACHUQ7_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
     gene            complement(5443..6021)
                     /locus_tag="ACHUQ7_001481"
     CDS             complement(5443..6021)
                     /locus_tag="ACHUQ7_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFR1134956.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVSGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(6044..6475)
                     /locus_tag="ACHUQ7_001482"
                     /pseudo
     CDS             complement(6044..6475)
                     /locus_tag="ACHUQ7_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
     gene            6847..7866
                     /gene="epd"
                     /locus_tag="ACHUQ7_001483"
     CDS             6847..7866
                     /gene="epd"
                     /locus_tag="ACHUQ7_001483"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFR1134957.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7916..9079
                     /gene="pgk"
                     /locus_tag="ACHUQ7_001484"
     CDS             7916..9079
                     /gene="pgk"
                     /locus_tag="ACHUQ7_001484"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFR1134958.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            9294..10373
                     /gene="fbaA"
                     /locus_tag="ACHUQ7_001485"
     CDS             9294..10373
                     /gene="fbaA"
                     /locus_tag="ACHUQ7_001485"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFR1134959.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            10564..11424
                     /gene="mscS"
                     /locus_tag="ACHUQ7_001486"
     CDS             10564..11424
                     /gene="mscS"
                     /locus_tag="ACHUQ7_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFR1134960.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            11563..12198
                     /gene="argO"
                     /locus_tag="ACHUQ7_001487"
     CDS             11563..12198
                     /gene="argO"
                     /locus_tag="ACHUQ7_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFR1134961.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            12291..13031
                     /locus_tag="ACHUQ7_001488"
     CDS             12291..13031
                     /locus_tag="ACHUQ7_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFR1134962.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            13198..14148
                     /locus_tag="ACHUQ7_001489"
     CDS             13198..14148
                     /locus_tag="ACHUQ7_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1134963.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATIFSILLKIVTGQTINPASSRLQLNYGVSRRRG"
     gene            complement(14090..15568)
                     /gene="scpC"
                     /locus_tag="ACHUQ7_001490"
                     /pseudo
     CDS             complement(14090..15568)
                     /gene="scpC"
                     /locus_tag="ACHUQ7_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
     gene            complement(15592..16377)
                     /gene="scpB"
                     /locus_tag="ACHUQ7_001491"
     CDS             complement(15592..16377)
                     /gene="scpB"
                     /locus_tag="ACHUQ7_001491"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFR1134964.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(16388..17383)
                     /gene="meaB"
                     /locus_tag="ACHUQ7_001492"
     CDS             complement(16388..17383)
                     /gene="meaB"
                     /locus_tag="ACHUQ7_001492"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFR1134965.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGIAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(17376..19520)
                     /gene="scpA"
                     /locus_tag="ACHUQ7_001493"
     CDS             complement(17376..19520)
                     /gene="scpA"
                     /locus_tag="ACHUQ7_001493"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HFR1134966.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSLF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCHTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(19724..20617)
                     /gene="argP"
                     /locus_tag="ACHUQ7_001494"
     CDS             complement(19724..20617)
                     /gene="argP"
                     /locus_tag="ACHUQ7_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFR1134967.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            21000..21659
                     /gene="rpiA"
                     /locus_tag="ACHUQ7_001495"
     CDS             21000..21659
                     /gene="rpiA"
                     /locus_tag="ACHUQ7_001495"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFR1134968.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            21915..23147
                     /gene="serA"
                     /locus_tag="ACHUQ7_001496"
     CDS             21915..23147
                     /gene="serA"
                     /locus_tag="ACHUQ7_001496"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFR1134969.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            23376..23435
                     /gene="ibsC"
                     /locus_tag="ACHUQ7_001497"
     CDS             23376..23435
                     /gene="ibsC"
                     /locus_tag="ACHUQ7_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFR1134970.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            23768..23827
                     /locus_tag="ACHUQ7_001498"
     CDS             23768..23827
                     /locus_tag="ACHUQ7_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR1134971.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(23928..24530)
                     /locus_tag="ACHUQ7_001499"
     CDS             complement(23928..24530)
                     /locus_tag="ACHUQ7_001499"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFR1134972.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(24551..24734)
                     /gene="ssrS"
                     /locus_tag="ACHUQ7_001500"
     ncRNA           complement(24551..24734)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHUQ7_001500"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24776..25105)
                     /gene="zapA"
                     /locus_tag="ACHUQ7_001501"
     CDS             complement(24776..25105)
                     /gene="zapA"
                     /locus_tag="ACHUQ7_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFR1134973.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            25273..25851
                     /gene="ygfB"
                     /locus_tag="ACHUQ7_001502"
     CDS             25273..25851
                     /gene="ygfB"
                     /locus_tag="ACHUQ7_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFR1134974.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            25877..27202
                     /gene="pepP"
                     /locus_tag="ACHUQ7_001503"
     CDS             25877..27202
                     /gene="pepP"
                     /locus_tag="ACHUQ7_001503"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFR1134975.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            27199..28377
                     /gene="ubiH"
                     /locus_tag="ACHUQ7_001504"
                     /gene_synonym="visB"
     CDS             27199..28377
                     /gene="ubiH"
                     /locus_tag="ACHUQ7_001504"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFR1134976.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            28400..29602
                     /gene="ubiI"
                     /locus_tag="ACHUQ7_001505"
                     /gene_synonym="visC"
     CDS             28400..29602
                     /gene="ubiI"
                     /locus_tag="ACHUQ7_001505"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFR1134977.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPTELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
BASE COUNT         7092 a         7432 c         8022 g         7156 t
ORIGIN      
        1 tttgaactgc cagaaattac cgataatgaa ctgttagtga gtgtaatttc tgacagcgtc
       61 tgtttatcga cctggaaagc ggcgttactc ggtagtgaac ataaacgcgt acccgacgat
      121 ttagaaaatc atccggtcat taccgggcat gaatgtgccg gggttattgt cgaagtgggt
      181 aaaaatctca ctggcaaata taaaaaaggc cagcgttttg tattgcaacc ggcgatgggg
      241 ttaccaagcg gatattcagc gggctacagc tacgaatatt ttggcggcaa tgccacttat
      301 atgattattc ccgaaatcgc cattaatttg ggctgcgtat taccgtatca cggctcttat
      361 tttgctgcgg cgtcgctggc agagcctatg tgctgcatta ttggtgctta tcatgccaat
      421 tatcacacca cgcaatatgt ttatgagcat cgcatgggcg tcaaacctgg cggcaatatt
      481 gcactgctgg cgtgtgcggg tccgatgggc attggcgcta tcgattacgc cattaacggt
      541 ggcatacaac cgtcacgggt ggtggtggtc gatatcgacg acaaacgcct ggcgcaggtg
      601 cagaagctgc tgccggtgga tctggcggcc agtaaaggca ttgagctggt gtatgtgaat
      661 accaaaggga tgagcgatcc tatccagatg ctgcgggcgc tgacaggaga tgtcgggttc
      721 gatgacattt ttgtttatgc ggcggtgcct gctgtcgttg agatggctga cgaattactg
      781 gcggaagatg gctgtctgaa cttctttgcc gggctgacgg ataaaaactt caaagtgccg
      841 tttaatttct acaacgtcca ttacaacagc acgcacgtag tcggtacatc cggcggttca
      901 acggacgaca tgaaagaggc gattgccctt agcgccacag gacagttaca gccgtcgttt
      961 atggtgaccc atattggtgg cctggatgcg gtgccagaaa ccgtgctcaa tctgccggat
     1021 atccctggcg gtaaaaaact catttataac ggcgtgacca tgccgctcac tgccattgcc
     1081 gattttgccg aaaaaggcaa aaccgatccg ctgtttaaag agttggcgcg gctggttgag
     1141 gaaacgcacg gcatctggaa tgaacaggcc gagaaatatc tgctggcaca atttggcgtt
     1201 gatatcgggg aggccgcgca atgatgtccc tggcgtggcc attatttcgc gttacggaac
     1261 aggcagcgtt ggctgcctgg ccgcaaaccg gatgtggcga caaaaataaa attgatggcc
     1321 tggcggtcac cgcgatgcgc caggcattaa acgacgtcgc ttttcgtgga cgagtggtta
     1381 tcggcgaagg agaaattgac catgcgccga tgctgtggat tggcgaagag gtgggcaaag
     1441 gcgatgggcc agaagtcgat atcgcggttg atcccattga aggcacgcgg atggtggcga
     1501 tggggcagag caatgcgctg gcggtcatgg ctttcgctcc tcgagatagc ctgttacatg
     1561 cgcccgatat gtatatgaaa aagctggttg ttaatcgact ggctgccggg gcgattgatc
     1621 tgtctttgcc tctggcggac aacttgcgta acgtggcgcg ggcgttaggt aaaccgctgg
     1681 acaagctgcg catggttacg ctggataaac cgcgtctgag tgccgcgatt gaagaagcaa
     1741 cacagctggg cgtgaaggtt tttgccctgc cggatggcga tgtcgccgcc agcgtgctga
     1801 cctgctggca ggataatcct tacgatgtga tgtacaccat cggcggtgcg ccagaaggcg
     1861 tgatttctgc ctgtgccgtt aaagcgttag gcggggatat gcaggccgag ctgattgact
     1921 tttgtcaggc taaaggggat tacacggaaa atcggcagat tgcagagcag gagcgcaagc
     1981 gttgtaaggc aatgggcgtt gacgttaacc gtgtgtactc gctcgatgaa ctggtgaggg
     2041 ggaacgatat cctctttagc gctacgggcg tgacgggcgg cgagttggtg aacggtatcc
     2101 aacagacggc gaatggggtg cggacgcaga cattactgat cggcggcgcg gaccaaacgt
     2161 gtaatataat agactccctg cattgatggt gatctatcgg aagaccaatg gcgacgctga
     2221 cagaagatga tgtgcttgag caactggatg cacaggacaa tttattttca tttatgaaaa
     2281 ctgcgcattc tattttgctc caggggatac gccagtttct gccgtcgctg tttgtcgata
     2341 acgatgaaga gatcgtcgaa tatgcagtga agccgttact cgcccaaagc ggcccgcttg
     2401 acgatattga tgttgcgctg cgtttgattt atgcgctggg gaaaatggat aaatggctgt
     2461 atgccgatat cacgcacttt tcccagtact ggcattacct gaacgaacag gatgaaacgc
     2521 ccggatttgc cgatgatata acctgggatt ttatcagcaa tgtcaacagt ataactcgca
     2581 atgcgacgct ctacgatgcg ttaaaagcga tgaagttcgc cgatttcgcc gtctggtcag
     2641 aggcgcgttt tagcggaatg gtcaaaacgg cgctgacgct ggcagtaacg accaccttaa
     2701 aggaattaac gccgtgaaaa ttgaattaac ggtgaatggg ctgaacgtgc aagcgcagta
     2761 ccatgatgaa gagatagaac gcgtgcataa actgttgctg cgtatgctgg cggccttaca
     2821 gacggtaaat ccgcagcggc gtacggtggt ttttctttgc gctccgccgg ggacgggtaa
     2881 atctacccta accaccttct gggaatatct cgcgcaacaa gacccggagc tgcccgctat
     2941 tcaggcgctc ccgatggatg gttttcacca ttacaatagc tggctggacg cgcatcaatt
     3001 gcgccccttc aaaggcgcac cagagacatt tgacgttgcg aaactggcgg aaaatctgcg
     3061 ccaggtggtg gaaggggatt gtacgtggcc gcagtacgat cgacaaaagc atgatcctgt
     3121 tgaagatgcg ttgcacgtta ccgcaccact cgtcatcgtc gaaggaaact ggttgttgct
     3181 ggacgacgag aagtggtgcc agcttgcgca attttgtgat ttctcgatct ttatcaacgc
     3241 gccagctgct gcgttgcggg agcgtctggt ggggcgcaag ctggcgggag gggtatcgct
     3301 ggcagatgca gaagcctttt atgaccgtac cgacgggccg aatgttcgcc gggggctgga
     3361 agagagtctg cctgcaaatt tgaccttaat gatgaccgct acgggtgaat atcgtctggt
     3421 ggattaacgt tcctgattac accatcttcc agccgcacca cacgagagaa atgacgccgg
     3481 gtaaatgcgg cgtcatgact gattgctacc acgctcgtgc ctcgctggcg gcacttctcc
     3541 agccagcttt caaacacgct caaccaattt tcgtcaaaat cccgactcgg ctcgtccagc
     3601 agtaatagtg gcggagagag tgcttccagg caggcgacgg cgaccatccg tcgctgcgca
     3661 ttatgcagat ccaacgggtg cgagttggca gtgtctgcta actgacaaca ttgcagggca
     3721 gcatgggtac gttgagtgat ttcatccgcc gggcattttt gcagtttcaa accaaatgcg
     3781 atctcgtcag cgacggtgct gtgaaaaagt tggttttccg cttcctgaaa cagtacgcca
     3841 acctttgccg cgcgctggcg gtttttcagg tttttcatcg cctgttgttg cagcataacc
     3901 gtaccggctg tgggcgtaag aagacctgcc attactcgta acagcgtcga tttccctgcg
     3961 ccattgtcac cggtcagcgc caaccattca ccttgtttga gttgcagaga aatgtcgcaa
     4021 agacaatccg ttgccgcgcc aggccagcga taagatatct tatttaacgt taacatagtg
     4081 gctggagcgc tccgttttgc aattgccacg cttgctggca gtagttcagg aaaggtgtgc
     4141 gatgacgttc aaaaagaata atcagcgaac cacgttcaaa cgcccagtgt tgtaagcgct
     4201 gcaacagcat ttcgctggcc tgcggtgtca ggcggctaaa ggcttcatca agaattaaga
     4261 gctttggctg catcgcgatg gcgcaggcga tgatcacgcg ctgtgtctct ccaccggaga
     4321 gtgttgccgg gtgacgatgg cgcaacggct ggcactcggt cagagtcagg gcggcatcaa
     4381 tccgcgccat aatctctgct tcagcaagac acagattttc tggcccaaag gcaacttctt
     4441 cttccacgct aaaggtacag ccggagagct gcaaataggg cgattgctgg acgagctgga
     4501 tggtggcgga ttgttcatta agcgggagat gcccaatggg cgtaccgagg agcgtccccg
     4561 tgcctgttat ctctcttggc agaaagtccg gataccagcc cgccatcagc tgtgccagcg
     4621 tacttttgcc actgccgttg tcaccaaaga tggcgaccat ccccggtttg acatagtgaa
     4681 agtcataaca gaacggagga tgcgttgagt gtgtcgggca ataacggaac tgttctaacg
     4741 taaccatatc cacgctccga attctgtcag catcagcaag atcatggtgt aacgcgctac
     4801 gcgttgtagg gtgctatcgg ctggtgccca caacgttgtt cgtcggttgt ggatccgaaa
     4861 tgcccgcata tccagcgccg caccacggac agccagatcg ttaagcgcat tgtgtgttag
     4921 cggaataatt aacgcgggca tagcacgtaa acgctgatac cagccctcat ccagcggcac
     4981 gccgcgggca cgctgcgctt catgaataat cgctaattgc cgttttaact gctcaacgac
     5041 caataacggc ccggcaaaca gataagcgac gcctggcggc aggcgagaag caaacagagc
     5101 gcgaataaaa cattgcacgg ggacgtactg cattcacaac tgtgaggttg aaacaatcgc
     5161 cagaatcagc aaccagagcg taatagcatg cgaccaccgt tctggcgagc gtggcgtgcc
     5221 gcttagccat tctggtagcc agccaccgtg taccagccac agcccggctc ccagcgagaa
     5281 catcagccag acgacatatt ttgcccgccg ccgcgtggcc tttagcgcaa taaggcagaa
     5341 aaaggtcgcc gcgctataga tcggcaaaat ggtctgcgcg ggcaggataa gcgtggtgca
     5401 ggccgctaat gcccacagtg ttaatgaggt aaatggatgc atttagcgca cggcagccat
     5461 tgccggaaaa tggcgcgtgg tgcgaatcgg cagctggcgc agtaaaagcc agacaatcac
     5521 cgctgtaaga attttatcga ccaggttcgc gcctatcacc gtaatcgcca ccgactccac
     5581 caggttttgc cccatcgagt gcatccaggc aacgaacaaa tcggctccgc taccggtgac
     5641 accgccgaac agcgcagtac gcagcggtac agcgacgacc gttaccgcaa gcgtaataat
     5701 cacgccgctg acaacgactt ttggcagggt gcgaaaccaa cccgctcgcg ccagccaacc
     5761 ggagaccagg ccgatgacca tcgcgacggg tgcaaatgcg gcggcgatcg ggtcggtcag
     5821 caatccccag agtaaattgg tcagtaagcc ggtcagcatt ccgatcaccg gaccaagcag
     5881 taccgcgcta ataagtgtgc cgatggagtc gagaaatata ggtaatttga ccatgctggc
     5941 cagttgcccg ccgatcatat tgatggcgat agatataacg atcaacacca gtgcctgact
     6001 ggaaaagtgg ctacgcgcca taagaaaacc tctttgaaag tagttattta acgggggaaa
     6061 tatgcaactc gtcgtaacca gaaagcgcgc aaggttggcg gctaaacgcc agttcctcaa
     6121 ggctaccgat caccagttca agcgtggtcc gcacggtaca gccgggcatc atatgaccgc
     6181 caagcatcgt gccgtgtgga tcggaaacgc agagatgaag atgctcacca ctctgttcca
     6241 gcgtgccgtt tagtgagatc acttcgaatg taccgttcag gagcgtggtg ccttcttgcc
     6301 cggcatagcg caaggcgacg tcagtcaggc taccggtaca gcctgcaatc taggcggcat
     6361 gaagttgatg ttgctgtgca aaagcgtgta gctgggaaaa aacttcctgc ccggggagga
     6421 ggcgcagtgc gtaaaagcgt gcggaggaag aatatgagta gggcaatgtc atcattggag
     6481 tactccgaaa ctcggacgta ttgcggataa tatcgacgct gcatcagtca tattctgata
     6541 taagtcaggg tttgtgacgg cacaacgaaa agattttgct acgcttattc gtgattctgc
     6601 aaagtggaat aaattccaca ggggcggggc tgaaaacgtg acgtaagtca gataattgct
     6661 ttctttgact ggacaatcat tccttttatt ccacgtttcg cttatcctag ctgaagcgtt
     6721 tcagtcgatt aaatgttcga caattaacca atcagtcgca gtttgcgaca ggtaaggttt
     6781 ccccggacga tttgctggat tactctgtct ggcaaatttg ctcagggaaa accttgcagg
     6841 agatctatga ccgtacgcgt agcgataaat ggcttcggtc gcatcgggcg taatgtggtt
     6901 cgtgctttgt atgaatccgg acgccgggcg gaaattaccg tggtggcaat caacgaactg
     6961 gcggatgctg cgggcatggc gcatttgttg aaatatgaca ccagccatgg ccgttttgca
     7021 tgggaagtac gacaggaacg cgatcaactt tttgttggtg atgacgccat ccgcgtattg
     7081 catgaacgtt cactgcaatc gctcccctgg cgtgaacttg gcgttgatgt agtcctcgac
     7141 tgcaccggcg tatatggctc ccgcgagcat ggcgaagcgc atattgccgc cggggccaaa
     7201 aaagtgctct tttcacatcc tggcagtaac gatctcgacg cgaccgttgt ttacggcgtc
     7261 aatcaggatc aacttcgtgc ggaacaccgc atcgtttcta acgcttcctg taccacgaat
     7321 tgcataattc ccgtcatcaa attgttagat gatgcgtacg gtattgagtc cggcactgtg
     7381 accacaattc actccgccat gcacgatcaa caggttattg atgcatacca tcctgacctg
     7441 cgtcgcaccc gggcagccag ccagtcgatc attccggtcg atactaaact ggccgctggt
     7501 atcacacgat tttttccgca atttaacgat cgctttgaag cgattgcggt acgtgtgcca
     7561 accataaatg tgacggcaat cgatttaagc gtgacggtga agaaacctgt aaaagccaat
     7621 gaagtcaacc tgttgctgca aaaagcagca caaggtgcat ttcatggtat agttgactat
     7681 acggaattgc cgttggtctc tgtagatttt aaccacgatc cgcacagtgc cattgtcgat
     7741 ggcacccaaa cccgggtcag tggcgcacac ctgatcaaaa cgttggtctg gtgcgataac
     7801 gaatggggct ttgctaaccg aatgctcgac acgacgttag ctatggctac tgttgctttc
     7861 aggtaagacg caagcagcgt ctgcaaaact tttagaatca acgagaggat tcaccatgtc
     7921 tgtaattaag atgaccgatc tggatcttgc tgggaaacgt gtatttatcc gtgcggatct
     7981 gaacgtacca gtaaaagacg ggaaagtaac cagcgacgcg cgtatccgtg cttctctgcc
     8041 gaccatcgaa ctggccctga aacaaggcgc aaaagtgatg gtaacttccc acctgggtcg
     8101 tcctaccgaa ggcgagtaca acgaagaatt ctctctgctg ccggttgtta actacctgaa
     8161 agacaaactg tctaacccgg ttcgtctggt taaagattac ctcgacggcg ttgacgttgc
     8221 tgaaggtgaa ctggttgttc tggaaaacgt tcgcttcaac aaaggcgaga agaaagacga
     8281 cgaaaccctg tccaaaaaat acgctgcact gtgtgacgtg ttcgtaatgg acgcattcgg
     8341 tactgctcac cgcgcgcagg cttctactca cggtatcggt aaattcgctg acgttgcgtg
     8401 cgcaggcccg ctgctggcag ctgaactgga cgcgctgggt aaagcactga aagaacctgc
     8461 tcgcccgatg gtggctatcg ttggtggttc taaagtatct accaaactga ccgttctgga
     8521 ctccctgtct aaaatcgctg accagctgat tgttggtggt ggtatcgcta acacctttat
     8581 cgcggcacaa ggccacgatg tgggtaaatc cctgtacgaa gctgacctgg ttgacgaagc
     8641 taaacgtctg ctgaccacct gcaacatccc ggttccgtct gatgttcgcg tagcaaccga
     8701 gttctctgaa actgcaccgg ctaccctgaa atctgttaac gatgtgaaag ctgacgagca
     8761 gatcctggat atcggtgatg cttccgctca ggaactggct gaaatcctga agaatgcgaa
     8821 aaccattctg tggaacggtc cggttggcgt gttcgaattc ccgaacttcc gcaaaggtac
     8881 tgaaatcgtg gctaacgcta tcgcagacag cgaagcgttc tccatcgctg gcggcggcga
     8941 cactctggca gcaatcgacc tgttcggcat tgctgacaaa atctcctaca tctccactgg
     9001 cggcggcgca ttcctcgaat tcgtggaagg taaagtactg cctgcagtag cgatgctcga
     9061 agagcgcgct aagaagtaaa aaatcacagg gcagggaaac ctgcacttgt ttcagcgcgc
     9121 ttttagagca cgcatttttt catactctaa ataattcgag ttgcaggaag gcgacaagag
     9181 agtgaatccc caggagctta cataagtaag tgactggggt gagcgaacgc agacgcagca
     9241 catgcaactt gaagtattat gagtataagg cccgacgata caggacaaga gacatgtcta
     9301 agatttttga tttcgtaaaa cctggcgtaa tcactggtga tgacgtacag aaagttttcc
     9361 aggtagcaaa agaaaacaac ttcgcactgc cagcagtaaa ctgcgtcggt actgactcca
     9421 tcaacgccgt actggaaact gctgctaaag ttaaagcgcc ggttatcgtt cagttctcca
     9481 acggtggtgc ttcctttatc gctggtaaag gcgtgaaatc tgacgttccg cagggtgctg
     9541 ctatcctggg cgcgatctct ggtgcgcatc acgttcacca gatggctgaa cattatggtg
     9601 ttccggttat cctgcacact gaccactgcg cgaagaaact gctgccgtgg atcgacggtc
     9661 tgctggacgc gggtgaaaaa cacttcgcag ctaccggtaa gccgctgttc tcttctcaca
     9721 tgatcgacct gtctgaagaa tctctgcaag agaacatcga aatctgctct aaatacctgg
     9781 agcgtatgtc caaaatcggc atgactctgg aaatcgaact gggttgcacc ggtggtgaag
     9841 aagacggcgt ggacaacagc cacatggacg cttctgcact gtacacccag ccggaagacg
     9901 ttgattacgc atacaccgag ctgagcaaaa tcagcccgcg tttcaccatc gctgcgtcct
     9961 tcggtaacgt acacggtgtt tacaagccgg gtaacgtggt tctgactccg accatcctgc
    10021 gtgattctca ggaatatgtt tctaagaaac acaacctgcc gcacaacagc ctgaacttcg
    10081 tattccacgg tggttccggt tctactgctc aggaaatcaa agactccgta agctacggcg
    10141 tagtgaaaat gaacatcgat accgataccc aatgggcaac ctgggaaggc gttctgaact
    10201 actacaaagc gaacgaagct tatctgcagg gtcagctggg taacccgaaa ggcgaagatc
    10261 agccgaacaa gaaatactac gatccgcgcg tatggctgcg tgccggtcag acttcgatga
    10321 tcgctcgtct ggaaaaagca ttccaggaac tgaacgcgat cgacgttctg taagtcattc
    10381 ctttctgctt atctcaaggc ccgctctgcg ggtctttttt tcgccaaaag caaggtgatt
    10441 cagatgagaa atctgtgatc tatttggcaa aattatgctt tattgtttac ccttgtcaga
    10501 ctgcccgtca taaggcggcg gagtgtattt ctccattttg agtcagttga aaaggaatat
    10561 tgaatggaag atttgaatgt tgtcgatagc ataaacggcg cgggaagctg gctggtagct
    10621 aaccaggcgc tgctgctaag ttatgcagta aacatcgtgg cggcactcgc gatcatcatc
    10681 gttggtttga ttatcgcgcg gatgatttcc aacgcggtga atcgcctgat gatctcccgt
    10741 aaaatcgatg ccactgttgc tgattttctt tctgcattag tccgttacgg tattatcgcc
    10801 tttacgctaa tcgctgccct gggacgcgtg ggtgtacaaa cagcgtcagt cattgctgta
    10861 ctcggtgccg caggcttagc tgttggtctg gctttgcagg ggtcactttc taacctggcc
    10921 gctggcgtgt tacttgtcat gttccgcccg ttccgtgccg gagaatatgt tgacctcggc
    10981 ggcgtagccg gtactgtgct gagtgtgcag attttctcca ccaccatgcg tactgcagac
    11041 ggtaaaatta tcgttattcc gaacggtaaa attattgccg gaaatattat taacttctcc
    11101 cgcgagccag ttcgccgtaa cgaatttatt attggcgtgg cgtatgattc cgatatcgat
    11161 caggttaagc agatcctgac caatattatc cagtctgaag atcgcatttt gaaagatcgc
    11221 gaaatgactg tgcgcctgaa cgaacttggt gcatcgtcga ttaatttcgt ggtccgcgtc
    11281 tggagcaaca gcggcgatct gcaaaacgtg tactgggatg tgctggagcg tattaaacgt
    11341 gaatttgatg ccgccggtat cagcttcccg tacccgcaaa tggatgtgaa ctttaagcgg
    11401 gtgaaagaag acaaagctgc gtaatcaacg ctgaggccag ataatactct ctggcctctc
    11461 tcttattagt tattctgatt gccaattaat attatcaatt tccgctaata acaatcccgc
    11521 gatatagtct ctgcatcaga tacttaattc ggaatatcca acgtgttttc ttattacttt
    11581 caaggtcttg ctcttggggc agctatgatt ctgccgctcg gcccacaaaa tgcttttgtg
    11641 atgaatcagg gcattcgtcg tcagtaccac attatgattg ccttactttg cgctatcagc
    11701 gatttggtcc tgatttgcgc ggggattttt ggtggtagcg cgttattgat gcagtcgccg
    11761 tggttgctag cgctggtcac ctggggcggc gtagtcttct tgctctggta tggttttggc
    11821 gcttttaaaa cagcgatgag tagtaatatt gagttagcta gtgccgaagt cctgaagcaa
    11881 ggcagatgga aaattatcgc caccatgttg gcagtgacct ggctgaatcc gcatgtttac
    11941 ctggatactt ttgttgtact gggcagcctt ggcgggcagc ttgacgtcga accaaaacgt
    12001 tggtttgcgc tcgggacaat tagcgcctct ttcctgtggt tctttggtct ggctattctc
    12061 gcagcctggc tggcaccgcg cctgcgcacg gcaaaatcac agcgcattat caatctggta
    12121 gtgggatgtg tcatgtggtt tatagccttg cagctggcga gagacggtat tgctcatgca
    12181 caagccttgt tcagttaggc gctatctgat ggaaaaataa aacagaggcg ctaagcttgc
    12241 ctccagaggt cctgaatttt gggcaagtca attaacacgg agagactaac gtgaagttca
    12301 aagttatcgc cctggcggca ttaatgggta ttagcgggat ggcagcgcag gctaacgaat
    12361 tgccggatgg accgcatatt gtcacctccg gtacggcaag tgtggatgcg gtgccagaca
    12421 ttgccactct tgcgattgaa gttaacgtgg ccgcgaagga tgccgctaca gccaagaaac
    12481 aggcagatga gcgcgtcgca caatacattt ccttccttga actcaatcag atcgcgaaaa
    12541 aagatatcag ctcagcgaat ttacgcaccc agccagatta tgactatcag gatggtaaaa
    12601 gtatcctgaa aggctaccga gctgtgagaa cggtggaagt cacgctccgt cagttagaca
    12661 aactgaattc cttgctggat ggcgcgctga aggcgggtct taacgaaatt cgttctgtgt
    12721 cgctgggcgt ggcgcagccg gatgcctata aagacaaagc gcgtaaggca gcgattgata
    12781 acgcgattca tcaggcgcag gaactggcga acggctttca tcgtaaactg gggccggtat
    12841 atagcgtgcg ctaccatgtt tccaactatc agcccagccc aatggtgcgg atgatgaaag
    12901 ccgatgccgc gccggtgtct gcccaggaaa cttacgagca ggccgctatt cagtttgatg
    12961 atcaggtcga tgtggtcttc cagttagaac ctgtggatca acaacccgct aaaacacctg
    13021 cagcacaata atacttgtag cccgacgtat tcgtcgggct aatgccttta cccgatattg
    13081 cctccttttt ctgtaatttt ctgtaaaacc atgacgtaat cacctatctt tgctcgcggt
    13141 ttcatcaagg ataggctgac tgaaacggtt aagcggacaa ggatgcgggg aggggggatg
    13201 gacattttta tttcgaaaaa gatgcgcaat ttcattttat tagcgcaaac caataatatt
    13261 gcccgggcgg cagaaaaaat ccatatgacg gcttcgccat ttggtaaaag cattgccgcg
    13321 ctggaagaac aaattggcta tacgctattt acccgcaaag ataataatat cagcctcaat
    13381 aaagccggtc aggaactgta tcaaaaactg ttcccggttt atcaacgact ttctgcgatt
    13441 gataatgaaa tccataactc agggcgtcgt tcacgagaaa ttgtgattgg catagataat
    13501 acttatccga caattatttt cgatcagttg attagtctcg gagataagta tgaaggcgtg
    13561 accgcccagc cagttgagtt cagcgaaaac ggcgttatcg ataatctttt tgaccgtcag
    13621 ttagatttta ttatctcccc acagcatgta tctgcacgag ttcaggaact ggaaaacctg
    13681 actatcagtg aattaccacc gctgcgattg ggctttctcg tttctcgccg ttatgaagag
    13741 cgtcaagagc aagagctgct tcaggagctg ccctggctac aaatgcgctt tcaaaaccgg
    13801 gctaacttcg aagcaatgat tgatgcgaat atgcgacctt gtgggatcaa tcccaccatt
    13861 atttatcgtc cctacagttt tatggccaaa atcagcgccg tggagcgcgg acattttctg
    13921 acggtgatcc cgcattttgc ctggcgactg gtgaatccgg caacgctgaa atattttgat
    13981 gcgccgcaca ggccgatgta tatgcaggaa tatctttatt caatcagaaa tcatcgttat
    14041 accgccacga tcttcagcat attgctgaag atcgtgacgg gacaaaccat taacccagca
    14101 tcgagccggt tgcaattaaa ttacggtgta agtcgaagac gtggctaaga tcgtggtgaa
    14161 tatgtccgcc gggcgcattt tccagatagc gatgcagata atcccgatac ataggatgtg
    14221 cacaattatc aatgatagtg cgggcgcgtt gaagcgggga aagaccgcgc agatcggcga
    14281 tcccttgttc ggtgatgatc actttgacgc tgtgttcgct gtgatcaaca tggctgcaca
    14341 ttggcacgac ggttgagatc ttgccttctt tagcaatcga cggagccata aagatcgaca
    14401 gatacgcgtt gcgttcaaaa tcaccgctgc cgccgatgcc gttcatcaga tcgaccccgg
    14461 ctacgtgcgt tgaatttgca tgcccgtaaa tatcaaactc caggccgacg ttcagagcga
    14521 tgacgcccag acgacggatg atttccgggt tattggaaat ctcctgcgga cgcaacacaa
    14581 tgcggctggc aaagtaatcc atattgtcgt aaatcttgcg cagggaatcg gccgagattg
    14641 tcaggctgga ggcgctggcc ccgctgattt tgccggtttc cagtaaatgc accaccgatt
    14701 cctgtagcac ttccgaatac atcatgaacg gaggaatttc cgggttttcc cccagacgtg
    14761 ccattaccgc attattgata ttgcccacgc cactttgcag cggcagaaat tccggcggaa
    14821 tacgcccatg cgccatttcc tgcaataaga acgtgaccac gttatcggca atctgctggc
    14881 acatgggatt ttgcttatcc agcatattac cggcgtcggg caagttggtt tccacgacgg
    14941 cgacaatctt tttcggatcg atttgcacat agcgggtacc gacgcgatcc attgcatgga
    15001 agatcgacac gctattgcgc cgcggtggcg cgccaggaat cacaatatct gccagttctg
    15061 caacgcgcgg atcgtgatag tgattaagtt caatgatcac tttcttcgct cgcagcagct
    15121 aggtcggcgc attaccgatc ccgctggtta accagactcg accatccggt gccagtgccg
    15181 atgcttcaat gacggcaaca tcaatgtcgc cgaagaaacc gtaattgacc atttgcgcca
    15241 cttcgctcaa atgcaggtca acgaaactca ccgcgccctg attgatcttt ttacgtaaac
    15301 tggacgatgt ttgatatggc gcacgccagg aaacagcatc ggcgtcagaa agtacatcgt
    15361 cagcggcggc gctgattgac gcccccgtca gcaggcgaat ttgatacggc tttttggcct
    15421 catgctgttc gttagctctg cgggcaatcg cggcgggtag ggctttcggc gaacccgctg
    15481 gggtaaagcc gctaaatgcc accatgtcgt tatgctggat aatttctgcc gcttcatcgg
    15541 cggtcatcct tgtccactga gtttccattt ttactccttc gttcgcaggg attaatgacc
    15601 aacgaaatta ggtttacgtt tttcgaggaa agcgttcatc ccttcctggt aatcttcgct
    15661 gtcataaacc gcgcggcgca tcccctgaat acgttcaaat tcatcggagt tcatggtgtg
    15721 tgcttcgccc agtacacgca gctcttcttt gataacggca atggctaacg gcgctttctc
    15781 agagatgtgg tgcgccattt gtaaggtgaa atcttccagt tcttccactt ccacaacatg
    15841 gttgaggatg ccgacagcca gcgcgcgctg ggcggtgatt ggcgaagcgg taaaaatcag
    15901 ctctttgaca atgtggaagc ccgcgtcgcg ggtcaggttg tgaatgccga ccaggttata
    15961 cgggacgccg aggtttacag gcgtcattga gaaggttgag gtactggcgg cgatgatcag
    16021 atcggaactc atgatcattt caaatgcgcc accccaaaca ctaccttcca ccatcgaaat
    16081 gatcggtttc gggaattttt ggatcatgcg ggtgatttga cgcaatggat catcatagga
    16141 gagcggatcg cgaccgccag acggcagttc gtgaatatcg tgacctgcgg agaagacttt
    16201 ggatccactt ggcgcccgca aaatgataca gcgaatttcc ggccggttga gatcgcttaa
    16261 cgcctgcata agatcatcaa taaagacttt acttaaggca ttaagttttc gtccatagtt
    16321 aaactcaatg actgccactt tgttgatagt ggcaacgttc acatactgat aagacataaa
    16381 aattccttta atcaaaatat tgcgtctgga taaattcact gagctgccgc aggccggtgc
    16441 gcggtgagag cgtattgttt ttgaccgcta aaagcgtctg gcggtaatag cgatcgaaat
    16501 cttcattcgc gaacaggtga ttcagtactt cttcttcggt ctgcttacgc agccattcca
    16561 ccgattgttg ttgccgcact tgttgtaaac gaccactggc agttagcgcg gttttgaagt
    16621 cgatgatggc gtgccagatc tcatcgattc cacgtttttc cagtgcgcta caagtcagac
    16681 cccgtggctg ccattcgtcg tatttacgtc gcagaatatg cagggcactc tcgtacatat
    16741 gccgggcaat ggcgacattg gtatggttat cgccatcgtc tttgttgata acgagcagat
    16801 cagccacttc catcagccct tttttaatgc cctgcagatc atcgccgcca ccggcaattt
    16861 gcaacgagat aaaacagtcc accatgcggg cgacttccgt ttccgactgc gcgatgccaa
    16921 ccgtttcgac aatcactacg tcataacccg ccgcttcgca taacagcatt aattcccgtg
    16981 ctcgctgact ggcaccgccc agatgaccgg aggagggtac cgggcgaata aacgccgcct
    17041 cggcacgcgc caggtcattc atgcgggttt tatccccgag aatgctaccg ccagtgaccg
    17101 ggctgctggg atcgaccgca ataacagcaa cctttaatcc ctcacgaatc aacaacatgc
    17161 caaaggcttc aagaaaggta cttttccccg cgccgggtgt gccggtaacg cccagtcgca
    17221 gggcgttacc acagaacggc ataatagcgt caagcagctg tgtacttagc gcctgatgac
    17281 gtgggtgacg gctttccacc agcgtcatgg cttgggcgag cgtggcacgc tcaccctgac
    17341 gtaagcggcg aatactttct gccagcgtgg cttcattaat catgatgctg gcttatcaga
    17401 ttcagtacgt cgcgcacact gtcgagcata ggtgtacctg gaccataaat cgccgccacg
    17461 ccgcgctctt gcaggaaggc gtaatcctgc ggcggaatga cgccacccgc gaccacgcag
    17521 atatcttcgc gtccccattt tttcagcgct tcgaccagtt ccgggatcag cgttttatga
    17581 ccggcagcca gtgaggatgc gcccactacg tgaacgtcgt tttctacggc taggcgggcg
    17641 atctcttcag gtgtagagaa catcgggctt aaatctacgt cgaaaccgag atcggaatag
    17701 gcgctggcga tcactttcgc gccgcgatcg tgtccatcct ggcccatctt agcgatcaga
    17761 atgcgcgggc gacgaccatt gtcggcaagg aactgctccg tttgcgcaac aatggcatcg
    17821 aactcggagg ccgatttctc agactgatga tagctttgcg caatcacgcc ggtaacacac
    17881 tggcttggca ccagataacg gtcgaaagcg acttccagcg catcggaaat ttcacccagg
    17941 gtggcgcgaa cgcgagcggc attaacagcg gcagccagca ggttttcgtt atgctgtgcg
    18001 gcgtgagtca gggcgttcaa cgcggcggtt acggcggcat catcacgggt ggcgcgaatg
    18061 cgttccagcg aagcaatttg ctcgttacgc accatcacgt tgtcgatctc aagtacatcg
    18121 gtttcgtctt cgtgatccag tttgtacttg ttgacaccaa cgatgacacg cttgccctgg
    18181 tcgatcagcg actgttcgcg cgctgaggcc tcttcgatca ttcgttttgg cagacctgct
    18241 tcgatcgctt tcgccatgcc accggcttcg tcgatctgtt ggataatagc tctggcttgt
    18301 ttgacgattt gatcggtcag cgactcaatg taataggatc cggccagtgg atcgacggtg
    18361 tggcagagtt ctgattcttc ctggatgatg atctgggtgt tgcgggcaat gcgtgctgag
    18421 aaatcggtag gcaaaccaag cgcttcgtca aaggcgttgg tatgcagtga ctgagtaccg
    18481 cccagcgtcg cagccagtgc ttcaatggtg gtgcggataa cgttgttata cggatcctgt
    18541 tcagtcaggc tccagcctga ggtctggcag tgggtacgca gcgccagtga tttcggatcc
    18601 tgtgcgccaa atccactgac cgcttcgctc cataaataac gtgccgcacg caacatggcg
    18661 acgttcataa acagatccat gccgataccg aagaataacg acaggcgagg agcgaagtca
    18721 tcaattttca gtccggcaga gattgctgct ttgatgtact caatcccatc ggcgagcgta
    18781 aatgctacct gctgcacgca gttggcaccc gcttccccca tgtggtaacc gctgatactg
    18841 atggtattaa atcgcggcat gttgccggaa caccaggcga tgatgtcggc gataatgcgc
    18901 attgacggtt ttggtgggta aatataggtg ttgcggcaga ggtactcttt gagaatatcg
    18961 ttttgaatgg tgccggtcag tttatcaggt gtaacacctt gctcttctgc ggcgacgata
    19021 taaaacgcca gtactggtag cactgcgcca ttcatggtca tcgaaaccga cattttatcc
    19081 agcgggatct ggtcgaacag gactttcata tcttccacgg tgtcgatagc gacgcccgct
    19141 ttgccgacgt cacccgccac gcgtgggtta tcggagtcgt agccacggtg ggtggcaagg
    19201 tcaaacgcaa cggaaagacc tttttgcccg gcggccaggt tacggcgata aaaagcgttg
    19261 gactcttttg ctgttgaaaa accagcatac tgacggatgg tccacggttg ggcggtatac
    19321 atagtggcac gcgggccacg aacgtagggc ggcaaaccag gaagggtacc tgtcacctcc
    19381 agattatcga gatcggcttc ggtatacagc ggcttgatgg cgatcccttc cgcggtttgc
    19441 tgaaccagcg agtcgacagt tttctcccga cggctcaatt ccttgttggc aagctgttgc
    19501 cactcctgct cgttagacat tatttgctcc gtaaaacgca atcaaaatga gggtatttgc
    19561 gcttacggtg agctttttac gtgggtttgt cgccagcaaa aaaaatgcat tcagacgtcg
    19621 ttttttgccg cctgagattt gtctattttg tgatgtgatt caaggaatac aaaaagaagt
    19681 gaggacaacg cctgatatgt gctatcaggc attatttgat ggattaatcc tgacgaagga
    19741 ctttgtgacc ataatcgagt aacgcatcag tgactttacg catcatgcgg ctttcaggag
    19801 caaagcggtg ccagtagagc atccgtcgtt gaaatagccc aggcgttaag tcaatcagtt
    19861 caccgctggc cagctctttc tcgatttgca ggtgcgggat catacagcag gtggtgccct
    19921 ggcgagcaag ttgtacgaac gcttctgaag aattaacgat atggcagggt acgctgccag
    19981 gaggaagatc gaagttttgc tgcaaaaagg cctggtgcat atcgtcaaga tggtcaaacg
    20041 cgaccactgg cgctttcaat aatgccgaac gcgttacgcc gttaggaaaa tatttttcgg
    20101 caaacggttt tgagctgacg aacagatagt cgagcgcacc gagtttatcg acaagacaac
    20161 tcggcagcgc ctgatgttga atactcaccg cgccgaccac ttcgccgcgg cgcagacgtt
    20221 cctgagtacg ggtttcatct tctacctgca agttgaggcg gataggcgaa tcagccaaca
    20281 caggagccag tgccggaagc aaccacgtcg ccagactgtc ggcgttgacc gccagtgaaa
    20341 gcagcagcgg agtcgaaccg gtttgttcat cgcccagcca ctcttcttcc agcaactcca
    20401 cctggcgcag cagtgccagc agtttttgcc cttgttccgt cgggcgcggc ggtacggtac
    20461 gcaccaacag cggctgcccg aacatatttt ccagttgctt aatgcgctgt gagacggctg
    20521 attgtgtaat gcacagcttt tgtgccgcgc gctcaaatcc tcgttcacgt atcaccgcat
    20581 ccagtgcctg taatgttctg tagtccgggc gtttcattgc tgttgtctgc tcctggtaaa
    20641 tgctttttcc tgcactatga cacaattttg tgtcaggttg catataccga agccgtcggc
    20701 gggtaacatt tgtatcaatg gttaaacgcg cagcttaaaa gatcagggat ttgcgctatt
    20761 gcatttcgct tagcgacttc gcgccctggt tatgttgaat ggcgtggcgt tattgcctct
    20821 gttcgcaccg tggagcggca tacccagacc gttgtatgct ttatttgcgg ggcgaaaggg
    20881 gatgcccgcc attgcgcgat attttatgga tgagttaacc acccggcttg ccaacggggt
    20941 ctgaatcgct ttttttgtat ataatgcgtg tgaaatttca taccacaggc gaaacgatca
    21001 tgacgcagga tgaattgaaa aaagcagtag gatgggcggc acttcagtat gttcagcccg
    21061 gtaccattgt tggtgtaggt acaggttcca ccgccgcaca ctttattgac gcgctcggca
    21121 cgatgaaagg ccagattgaa ggggccgttt ccagttcaga tgcttccact gaaaaactga
    21181 aaagcctcgg cattcacgtt tttgatctca acgaagtcga cagccttggc atctacgttg
    21241 atggcgcaga tgaaatcaac ggccacatgc aaatgatcaa aggcggcggc gcggcgctga
    21301 cccgtgaaaa aatcattgct tcggttgcgg aaaaatttat ctgtattgcc gacgcctcca
    21361 agcaggttga tattctgggt aaattcccgc tgccagtaga agttatcccg atggcacgta
    21421 gtgcagtggc gcgccagctg gtgaaactgg gcggtcgtcc ggaataccgt cagggcgtgg
    21481 tgaccgataa tggcaacgtg atcctcgacg tccacggcat ggaaatcctt gacccgatag
    21541 cgatggaaaa cgccataaat gcgattcctg gcgtggtgac tgttggcttg tttgctaacc
    21601 gtggcgcgga cgttgcgctg attggcacac ctgacggtgt caaaaccatt gtgaaatgat
    21661 ctgacggggg aacctccccc gttaaaaaaa ttctcttcat taaatttggt gacatgtgtc
    21721 acgcttttac caggcaattg tcgattgctc taaataaatc ctctaaacca gcatattcat
    21781 ccaagaatta cctttgcgtg atatttcctc aacatcgcga cgcaaacgtt catattgccg
    21841 caatattatt ttttgatatg ttgaaaggcg gatgcaaatc cgcacacaac atttcaaaag
    21901 acaggattgg gtaaatggca aaggtatcgc tggagaaaga caagattaag tttctgctgg
    21961 tagaaggcgt gcaccaaaag gcgctggaaa gccttcgtgc agctggttac accaacatcg
    22021 aatttcacaa aggcgcgctg gatgatgaac aattaaaaga atccatccgc gatgcccact
    22081 tcatcggcct gcgatcccgt acccatctga ctgaagacgt gatcaacgcc gcagaaaaac
    22141 tggtcgctat tggctgtttc tgtatcggaa caaaccaggt tgatctggat gcggcggcaa
    22201 agcgcgggat cccggtattt aacgcaccgt tctcaaatac gcgctctgtt gcggagctgg
    22261 tgattggcga actgctgctg ctattgcgcg gcgtgccgga agccaatgct aaagcgcacc
    22321 gtggcgtgtg gaacaaactg gcggcgggtt cttttgaagc gcgcggcaaa aagctgggta
    22381 tcatcggcta cggtcatatt ggtacgcaat tgggcattct ggctgaatcg ctgggaatgt
    22441 atgtttactt ttatgatatt gaaaacaaac tgccgctggg caacgccact caggtacagc
    22501 atctttctga cctgctgaat atgagcgatg tggtgagtct gcatgtacca gagaatccgt
    22561 ccaccaaaaa tatgatgggc gcgaaagaga tttcgctaat gaagcccggc tcgctgctga
    22621 ttaatgcctc gcgcggtact gtggtggata ttccggcgct gtgtgatgcg ctggcgagca
    22681 aacatctggc gggggcggca atcgacgtat tcccgacgga accggcgacc aatagcgatc
    22741 catttacctc tccactgtgt gaattcgaca acgtccttct gacgccacac attggcggtt
    22801 cgactcagga agcgcaggag aatatcggct tggaagttgc gggtaaattg atcaagtatt
    22861 ctgacaatgg ctcaacgctc tctgcggtga acttcccgga agtctcgctg ccactgcatg
    22921 gtggtcgtcg tctgatgcac atccacgaaa accgtccggg cgtgctaact gcgctgaaca
    22981 aaatcttcgc cgagcagggc gtcaacatcg ccgcgcaata tctacaaact tccgcccaga
    23041 tgggttatgt agttattgat attgaagctg acgaagacgt tgccgaaaaa gcgctgcagg
    23101 caatgaaagc tattccgggt accattcgcg cccgtctgct gtactaattc cccttctctg
    23161 aaaattaacg ggcaggtcac tgacttgccc gtttttttat cccttctcca caccgttgcc
    23221 gcgttgcgca ttctactttg cgagtctgct ccagaaaata aatcaaagtc agatcaatgc
    23281 gttgtgttta tttaatgggg atttacgatg gcgggacagc atggggctgt aacggtaaag
    23341 ccctcaccga agcgagggct taaaggagaa gggttatgat gcgacttgtc atcatactga
    23401 ttgtactgtt actgataagt ttcggagctt attaacagtc agtctcaagg gaggagtcat
    23461 cctcccttac ccttactcac taaattaggt caaagaatca acgatgtcaa tcagggcgat
    23521 gcggggtgca tcgccctgaa ccgaactttt tgatagccac ttcatcgccc acgtttactg
    23581 ccgtaataga tcctttttcc caaaaattat ctctgttttg catggggttt ttcgagcacg
    23641 ctcgaagaaa agatttaaag gtagatcaat gcgttgtggt tatttaaaga gagcttacga
    23701 tagctgggca gcatggggct gtaacggtaa agccctcacc gaagcgaggg cttgaaggag
    23761 aagggttatg atgcgacttg ccatcatact gattgtactg ttactcataa gtttcccagc
    23821 ttattaacag tcagtcacag gggaggagca atcctccctt acccttactc actaaattag
    23881 gttaaagaat caacgatgtc aatcagggcg atgcaggtgt atcgccctta ccactcccag
    23941 actttcgacg gtgtaaccac cgcaggaaga gggatatccc actcttcaac ggggagtttt
    24001 tccaccaact gacaatcatg cgcataaccc accggttgcg ttttatagtg ctgccagttt
    24061 tgtaaggtcc gatcataaaa accaccgccc attcccagac gctgaccgta ctcatcaaag
    24121 gcgaccagcg gtgtgatcag cacgtctaat cgggaaaggg gtagcacgtc acgcacatcc
    24181 agttttggct catggatctt caacctgttc atcaccagtt cgctttgcgg atggtaattc
    24241 aggaacagca aattaccggc actaaaggga tgcaaaactg gaagatatac gcgcttaccg
    24301 gcgcgccaga gttgttctat tagcggctgg gtgtcgagtt caccatcaaa agagaggaat
    24361 acagcgaccg tatgcgccat caccaccggg ggataagtca tcatccgggt agcggcttgt
    24421 tgacccattt ccagctgttg ttccggcgtt aacgcacgac gacgttgccg aatcattttg
    24481 cggatttctt gtcgggataa tgtcagtggg agctctggta gttgcgtcat ggctggtgcc
    24541 agataagaag ggaatctccg agatgccgcc gcaggctgta acccttgaac ccttggttca
    24601 aggtgaatgt gtcgtcgcag ttttaaggct tctcggacga accgagcatg ctcaccaacc
    24661 gcggagcgcc acattcttgt ggtatgaaat atcggctcag gggactggcc cgcttgcgaa
    24721 catctcagag aaattttgtc ttcacggtta ctctaccaca gtaaaccgaa aagtgtcatt
    24781 caaagttttg gttagttttt tcggtgatgc gaccttgttc aagtaacgct tgttctatgg
    24841 tctgctgcag catccgaata cgctgttcca tacttgccgc gtagtcacga gtctttgctt
    24901 tttcttgcgc taactcatag ctgatattca atgcggcaat gaagaccaac tgttcagtat
    24961 ttgtgactct agtgcgttct ttcagatctt gcaaccgttg gttcagatcg tccgctgcct
    25021 gattcaacgc atccctttgg tcaggcgggc agttcacacg cagtgaacgg ccaaaaattt
    25081 ggatatcgac gggttgtgca gacatgccac cttcctgctg attgactgcg ctgccttcgt
    25141 ctttagaccc atggtctgcg aaggggcgac actatagcta ccctgatgag aagagacaag
    25201 cccttttctg gtccaccagg gcccaaagtg gtagcatatc atgaatattc ctccctttga
    25261 cgacgaatgc ttatgtctat acagaacgaa atgcctggtt acaacgaaat gaaccagtat
    25321 ctgaaccaac aagggacggg tctgacccct gccgagatgc atggtttaat cagtgggatg
    25381 atatgtggcg gtaacgatga cagctcatgg ctgccgctac ttcacgacct gacgaacgaa
    25441 ggcatggctt tcggtcatga gctggcacag gcactgcgta aaatgcactc tgccaccagc
    25501 gatgccctgc aggatgacgg cttccttttt cagctttatc tgcctgatgg cgatgatgtc
    25561 agcgttttcg atcgggctga tgcactggca ggttgggtca atcacttcct gcttggtctt
    25621 ggcgttacgc aaccgaagct ggacaaagtg accggcgaaa ccggtgaagc catcgacgat
    25681 ctgcgtaaca tcgcgcagct gggctacgac gaagacgaag atcaggaaga gcttgaaatg
    25741 tcgcttgaag agatcatcga atacgttcgt gttgccgcgc tgttatgcca cgacaccttt
    25801 actcatccgc aaccgaccgc gccagaagta caaaaaccga ctctacacta aaaacaaaaa
    25861 acgtaaggag agtgttatga gtgagatatc ccggcaagag tttcagcgtc gccgtcaggc
    25921 cctggtggag caaatgcaac ccggcagcgc cgcgctgatt tttgctgcac cagaagtaac
    25981 acgtagcgcc gacagcgaat acccctatcg tcagaacagt gacttctggt acttcaccgg
    26041 ctttaacgaa ccggaagcgg tgctggtgct gattaaaagc gatgacactc ataaccacag
    26101 cgttctgttt aaccgcgttc gcgacctgac ggcggagatc tggtttggcc gtcgcttagg
    26161 ccaggatgcc gcgccagaga aactgggcgt tgaccgcgca ctggcattca gcgaaatcaa
    26221 tcagcaactt tatcaactac ttaacggcct ggatgtggtt taccatgccc agggcgaata
    26281 tgcatatgct gatgaaatcg tgaacagtgc gctggaaaaa ctgcgtaaag gttcgcggca
    26341 aaatctcacc gcaccggcaa cgatgatcga ctggcgtcct gttgttcatg aaatgcgcct
    26401 gttcaaatcg ccagaagaga ttgccgtact ccgccgcgcg ggagaaatca ccgccatggc
    26461 acatacacgg gcgatggaaa aatgccgtcc gggaatgttc gagtaccatc tggaaggcga
    26521 aattcaccac gaatttaacc gccacggtgc gcgctatcct tcctataaca ccattgtagg
    26581 cagcggtgaa aacggctgca ttctgcacta caccgaaaac gagagtgaac tgcgcgacgg
    26641 tgacctggtg ttgattgacg cgggttgtga atacaaaggt tacgcgggcg atattacccg
    26701 caccttcccg gtcaacggca aattcaccca ggcccagcgt gaaatctacg acattgtgct
    26761 ggagtctctc gaaaccagcc tgcgcctgta tcgtccggga acttccattc tggaagtcac
    26821 tggtgaagtg gtgcgcatca tggttagcgg cctggtaaaa ctcggcatcc tgaaaggtaa
    26881 tgttgatgaa ctgatcgctc agaacgccca tcgtcctttc tttatgcatg gccttagcca
    26941 ctggttagga ctggatgtcc atgacgttgg cgtttatggt caggatcgct cgcgcattct
    27001 ggaaccgggg atggtactga ccgtagagcc tggtctgtat atcgcgccgg atgcggatgt
    27061 gccagaacaa tatcgcggta tcggcattcg tattgaagac gacattgtga ttaccgaaac
    27121 cggcaacgaa aacctcaccg ccagcgtggt gaaaaagccg gaagaaatcg aagcgttgat
    27181 ggctgctgcg agaaagcaat gagcgtaatc atcgtcggtg gcggcatggc gggcgcgacg
    27241 ctggcgctgg ctatttcccg gttaagtcac ggggcgctgc cggtacattt gattgaagcg
    27301 actgcgccag agtcacatgc tcatccgggc tttgatggac gagcgatagc gctggcggcg
    27361 ggtacctgtc agcaactggc gcgcatcggc gtctggcaat ctctggctga ttgcgcaacc
    27421 gccatcacta ccgtgcatgt cagcgatcgt ggtcatgctg gattcgtcac cctcgccgca
    27481 gaagattacc aactggcggc gctggggcag gttgtcgaat tgcataatgt cgggcaacgg
    27541 ctgtttgcat tgctgcgaaa agcacctggc gtaatgctgc attgccccga tcgcgtagct
    27601 aacgttgccc gtactcagag tcatgttgaa gtgacgctgg agagcggcga gatgctgacg
    27661 ggccgcgtgc tgatagccgc tgatggcacc cattcagcgt tagccaccgc gtgcggcgtt
    27721 gattggcagc aggagcctta cgaacaactg gcagtgattg ccaacgttgc tacttctgtt
    27781 gcgcacgaag gccgcgcttt tgaacgcttc acgcaacatg gcccgctggc gatgttgccg
    27841 atgtctgacg gacgctgttc gctggtctgg tgtcatccac tggaacggcg cgaagaggtg
    27901 ctgtcgtgga gcgacgagaa gttttgccgt gaactccagt ctgcctttgg ctggcgactg
    27961 gggcaaatta cccacgccgg taaacgcagt gcttatccgc tggcattaac ccgcgccgcc
    28021 agaccgatta cccatcgcac tgtgctggtg ggcaatgcgg cgcaaactct gcacccgatt
    28081 gccgggcaag ggtttaacct cggtatgcga gatgtcatga gccttgcgga aaccctgact
    28141 caggcgcatg agcgcggaga agacatcggt gattacggca tattgtgccg ctatcagcag
    28201 cgtcgacaga gcgatcgcga agcaaccatc ggcgtcacgg acagcctggt acatcttttt
    28261 gccaaccgtt gggcaccgct ggttgtcggg cgcaacatcg gactgatgac gatggaatta
    28321 ttcaccccgg cacgcgatgt gctggcgcag cgcaccctcg gttgggtggc gcgttgaagg
    28381 cggttttaag gaataaacaa tgcaaagtgt tgatgtagcc attgttggcg gcggcatggt
    28441 ggggctggcg gttgcctgcg gcttacaggg gagcggctta cgcgttgccg tactggagca
    28501 gcgcgtaccg gaacctctgg cggtagatgc actaccacaa ctgcgcgttt cggctatcaa
    28561 tgccgccagc gaaaaattac tcacccgtct tggcgtctgg caggacattc tcgctcgtag
    28621 ggccagttgt tatcacggca tggaagtgtg ggacaaagac agttttggtc acatctcgtt
    28681 tgacgatcaa agcatgggct atagccatct tgggcacatc gttgaaaact cagtgattca
    28741 ctacgcgctg tggaacaaag cgcagcagtc gtcagatatt actctgttgg cccccacaga
    28801 attacagcag gtcgcctggg gagaaaatga aaccttcctg acgctgaaag atggcagcat
    28861 gttaacggcg cgtctggtga ttggcgcgga cggtgctaat tcctggttgc gcaacaaagc
    28921 agatattccg ctgactttct gggattatca gcatcacgcg ctggtagcga ccattcgcac
    28981 ggaagaaccg catgatgcgg tggcgcggca ggttttccat ggcgaaggca ttctggcctt
    29041 tttaccgctt agcgatccgc atctttgctc gattgtctgg tcactgtcgc cagaggaagc
    29101 gcagcggatg cagcaggcaa gtgaagacga atttaatcgc gcgttaaata tcgcttttga
    29161 taatcgcctt ggcttatgca aggttgagag cgcgcgtcag gtgttcccgc tgacggggcg
    29221 ttatgcgcgc cagtttgccg cacaccgtct ggcgctggtg ggcgacgccg cgcataccat
    29281 tcacccactg gcggggcagg gcgttaatct cggatttatg gacgccgcag agctgattgc
    29341 tgaactgaaa cggttgcatc gtcagggtaa agacatcggg cagtacattt acctgcgccg
    29401 ctatgaacgt agccgcaagc acagcgcggc gctgatgctg gctggtatgc agggttttcg
    29461 cgatctcttt tccggcgcta atccggcgaa aaaactgctg cgtgatattg gtttgaaact
    29521 ggccgacacg cttcctggcg ttaagccgca acttatccgc caggcaatgg gattaaacga
    29581 tttgcctgaa tggctgcgtt aaaaatttct cctctgttgt ttatttgata cccatcacac
    29641 tttcatctcc cggttttttc gccgggagat tttcctcatt tgaaataaac taatttcacc
    29701 tc
//
LOCUS       DAZPCS010000034        28197 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZPCS010000034 DAZPCS010000000
VERSION     DAZPCS010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28197
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.034"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="ACHUQ7_001506"
     CDS             complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="ACHUQ7_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFR1134978.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERTSSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERIRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQTLPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQI"
     gene            complement(2138..2584)
                     /locus_tag="ACHUQ7_001507"
     CDS             complement(2138..2584)
                     /locus_tag="ACHUQ7_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFR1134979.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            2707..4761
                     /gene="helD"
                     /locus_tag="ACHUQ7_001508"
     CDS             2707..4761
                     /gene="helD"
                     /locus_tag="ACHUQ7_001508"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFR1134980.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSGGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="ACHUQ7_001509"
     CDS             complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="ACHUQ7_001509"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFR1134981.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(5347..6009)
                     /locus_tag="ACHUQ7_001510"
     CDS             complement(5347..6009)
                     /locus_tag="ACHUQ7_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFR1134982.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            6182..6595
                     /locus_tag="ACHUQ7_001511"
     CDS             6182..6595
                     /locus_tag="ACHUQ7_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFR1134983.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="ACHUQ7_001512"
     CDS             complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="ACHUQ7_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFR1134984.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="ACHUQ7_001513"
     CDS             complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="ACHUQ7_001513"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HFR1134985.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            8300..8578
                     /gene="yccX"
                     /locus_tag="ACHUQ7_001514"
     CDS             8300..8578
                     /gene="yccX"
                     /locus_tag="ACHUQ7_001514"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFR1134986.1"
                     /translation="MSKICIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="ACHUQ7_001515"
     CDS             complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="ACHUQ7_001515"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFR1134987.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="ACHUQ7_001516"
     CDS             complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="ACHUQ7_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFR1134988.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMIANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(9861..9948)
                     /locus_tag="ACHUQ7_001517"
     tRNA            complement(9861..9948)
                     /locus_tag="ACHUQ7_001517"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9912..9914),aa:Ser,seq:tga)
     gene            10374..11492
                     /gene="hyaA"
                     /locus_tag="ACHUQ7_001518"
     CDS             10374..11492
                     /gene="hyaA"
                     /locus_tag="ACHUQ7_001518"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFR1134989.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            11489..13282
                     /gene="hyaB"
                     /locus_tag="ACHUQ7_001519"
     CDS             11489..13282
                     /gene="hyaB"
                     /locus_tag="ACHUQ7_001519"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFR1134990.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            13301..14008
                     /gene="hyaC"
                     /locus_tag="ACHUQ7_001520"
     CDS             13301..14008
                     /gene="hyaC"
                     /locus_tag="ACHUQ7_001520"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HFR1134991.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            14005..14592
                     /gene="hyaD"
                     /locus_tag="ACHUQ7_001521"
     CDS             14005..14592
                     /gene="hyaD"
                     /locus_tag="ACHUQ7_001521"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFR1134992.1"
                     /translation="MSEQHVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            14589..14987
                     /gene="hyaE"
                     /locus_tag="ACHUQ7_001522"
     CDS             14589..14987
                     /gene="hyaE"
                     /locus_tag="ACHUQ7_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFR1134993.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            14984..15841
                     /locus_tag="ACHUQ7_001523"
     CDS             14984..15841
                     /locus_tag="ACHUQ7_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFR1134994.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            15975..17519
                     /gene="appC"
                     /locus_tag="ACHUQ7_001524"
     CDS             15975..17519
                     /gene="appC"
                     /locus_tag="ACHUQ7_001524"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFR1134995.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            17531..18667
                     /gene="appB"
                     /locus_tag="ACHUQ7_001525"
     CDS             17531..18667
                     /gene="appB"
                     /locus_tag="ACHUQ7_001525"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFR1134996.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSHPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNKNELY"
     gene            18679..18771
                     /gene="cbdX"
                     /locus_tag="ACHUQ7_001526"
     CDS             18679..18771
                     /gene="cbdX"
                     /locus_tag="ACHUQ7_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFR1134997.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            18851..20149
                     /gene="appA"
                     /locus_tag="ACHUQ7_001527"
     CDS             18851..20149
                     /gene="appA"
                     /locus_tag="ACHUQ7_001527"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFR1134998.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESMVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTVFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(20264..22430)
                     /gene="etk"
                     /locus_tag="ACHUQ7_001528"
                     /pseudo
     CDS             complement(20264..22430)
                     /gene="etk"
                     /locus_tag="ACHUQ7_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
     gene            complement(22450..22896)
                     /gene="etp"
                     /locus_tag="ACHUQ7_001529"
                     /gene_synonym="yccY"
     CDS             complement(22450..22896)
                     /gene="etp"
                     /locus_tag="ACHUQ7_001529"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFR1134999.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYSMLERASQEWAKRLSR"
     gene            complement(22884..24023)
                     /locus_tag="ACHUQ7_001530"
     CDS             complement(22884..24023)
                     /locus_tag="ACHUQ7_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFR1135000.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(24069..26165)
                     /locus_tag="ACHUQ7_001531"
     CDS             complement(24069..26165)
                     /locus_tag="ACHUQ7_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFR1135001.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAWQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGKSEQLQQQRVEAEDLSAFGRCYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDDGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKLFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLDPMTAEREVPVGQ"
     gene            complement(26165..26911)
                     /locus_tag="ACHUQ7_001532"
     CDS             complement(26165..26911)
                     /locus_tag="ACHUQ7_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFR1135002.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPSEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(26908..27552)
                     /gene="gfcB"
                     /locus_tag="ACHUQ7_001533"
     CDS             complement(26908..27552)
                     /gene="gfcB"
                     /locus_tag="ACHUQ7_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFR1135003.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVAAQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNPGRAPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGHNV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(27659..27964)
                     /locus_tag="ACHUQ7_001534"
     CDS             complement(27659..27964)
                     /locus_tag="ACHUQ7_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1135004.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTSGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
BASE COUNT         6783 a         7187 c         7452 g         6775 t
ORIGIN      
        1 taatctggcg ttgcagacga ccaatctctg gcagtaatgc aatcaataac ccaacttgtt
       61 gtacgaccag aggttctttg ctatctgccc gtggttcaag ttgctgcatc cgctgtttca
      121 ggccagctaa tgcctgattg acgcgttctt catcagcagg ttgatgatgt aacgcgtcat
      181 caacatagca aactgcgtca tcaagaaacg ccagaatttc aggattagtt aactgctccc
      241 ggtgagcacc gagggctgag atatagctgg taaacgtatg gttaaggcac agcaagcgaa
      301 acgcggcttc gcgaatttgc ggggtaacgt tcggttcgct ggacatattt gataccaccg
      361 acgccagttc agcatcacgg ttgtgtgcat cgcggcgggc aatacgatac gccagacggt
      421 tatcacgccc ctgatggtat tgctccagta tggcatcgag atagcgacag ttggcctctg
      481 tggcgcgttc gagcattcgc ggcagattgc gaaacttcca gtcaggccag atgtagctca
      541 ctgccgccca cgcaatggca caaccaatca gcgtatcgat tacgcgaggt aacgctactt
      601 caaaaccttc acccagtaag ttaaaacaca gtagcaccaa aagtgtgatg aacatcgttg
      661 catgagcgta ttgcacgtta cggaaggcaa aaaagagcac gccggtaata accagcagca
      721 ccagctgccc ttccagtgat ggcacaaacc acagcacagg aatgccaatc gcgataccta
      781 ccagcgtacc aataatcctt aacttcaggc ggtggcgcgt ggcgttatag tttggctggc
      841 agacaaacaa acttgtcagc aagatccaat acccgtgatg cattccggtt atttgaatga
      901 tggcgtagcc gaagcacaac accagcgaca ttcttaccgc atgacggaag agggcggatt
      961 ccggcgtgaa gtgacggcta agacgcagcc agatatcact caatccgtgc gggctgtcat
     1021 cagcgagctc attttcgtca ttattacggg gtagtgtctg ggcctgttct gattcaattg
     1081 ttgccagttg ggcatcaatg gcgcgtaaat tgttcagcaa aaatcccagt gttttgagta
     1141 aatcggcggg tgcgccgtta tcgcgtatcc gctccagcgc agcatcaata tgcgtaaaag
     1201 cgcgctcaaa atgcggatca tgttgataag actgacgcaa caaaatacag cgtgacagtt
     1261 gctggcacgc ctggccctgc atcgacatca gccgctgaaa acggaacagc acgtcgctgt
     1321 ggcgaaaatg ttcacgcaat gtttgatact gaatatgaga agagctggta cgctcgtgaa
     1381 tatcctgtgc gacaaagtaa taatgcagcg tgcgacgcgt tccccgttga ccacgatcgc
     1441 cacgtaagcg ggtcagcagc gagagtttcg tctgattcaa tgtcgccatc agctgaccgt
     1501 tggcgagagc caaatcgtac agcggtgcct ggctttcatc ttcaatatca ggatcaaaca
     1561 tgcgcgactt gagctcaaga taacgcgcca gttgttcata gcaacgcgcc aggttgtcct
     1621 gcagcgggcg gaccgggaac agcagatgac caataagtgt caggacgttg taccagacgg
     1681 caccggccag cagatacatc ggctgctgat accagtgctc atacagtgat gttcccaaca
     1741 tagtgtaaat ggcgatcagc aatgcaccga aggcaattgt tgcatagcgt tgacccagac
     1801 cgccgagcaa aatgaagccg ctggtagaga gcgttaagcc aatcgcaaat agccagggcc
     1861 agggaaacag caattctact gaggccgagg cgataaaaaa gcagaacagc gtaatgatga
     1921 ggttacgcaa acgtcccgcc agtcggtcat cgagatcggt cagcgccgct gccaccatcc
     1981 ccagcgttag cggaatcgtc agttttacat cacccagcca ccacggaaac gctgtggttc
     2041 cacaaagcgc aataaaaata cgcgcgtaat acagccaggc gctgttccag gtatagcgtt
     2101 tgagcaaagg acttagcata aaggccatat tagtggatta ttcaaaacga cgacgtgcat
     2161 tggcttcacg cgcagcttgc gctgtttcta ccgataccac gcgacgacca accggccata
     2221 gtgcaatagc ggcaatttta aagttcgcaa tgccgacagg aatgccaatg attgaaatac
     2281 attgtgcgat gcccgttgca atgtgcatca ggcataacca ccagccaaag aaaatcagcc
     2341 agaaaatatt caataccgta ccgccagtat tcagcaacac atttttgcca gccgggttca
     2401 gttcatcgac atgaatagct tcattgccat aaggcaccag agacagttta gtgatctccc
     2461 agcaggatcg tgtcagcggt aaggtaaaaa tcagcacaat actgaccaga gtcgccaaca
     2521 gccagcccag agtggtggca aatccgccaa gcacaaagtt cagaatgttc aaaacggtac
     2581 gcataaaacc tcgctttact gtggttttca gtaatgggcg gcaattgtaa cgtttttttg
     2641 ggctggagca cgttttctct gacggttaca ctgataagaa ataatttcgt gtggatctac
     2701 agagtcatgg aactgaaagc gacaacgctt ggaaaacgtc tggcacagca cccttacgat
     2761 cgggcggtga tcctcaatgc cgggattaaa gtctccggcg atcgccacga ataccttatt
     2821 cctttcaatc aattactggc gattcactgt aagcgcggtc tggtatgggg cgagctggaa
     2881 tttgtactgc cggacgaaaa agtggtgcgt ctgcacggca cagaatgggg cgagacgcag
     2941 cgtttttacc atcatcttga tgctcactgg cggcggtgga gtggcgagat gagcgaaatt
     3001 gcgtctggtg ttttacgcca gcaactggat ttgattgcca cgcgcactgg agaaaataaa
     3061 tggctgacgc gtgagcaaac ctctggcgtt cagcaacaaa tccgccaggc tttgtcggcg
     3121 ttgccgttgc cggttaaccg actggaagaa ttcgataact gccgtgaggc gtggcgtaaa
     3181 tgtcaggctt ggttgaaaga tattgaaagc gctcggttgc agcataacca ggcgtatacc
     3241 gaagccatgc ttaccgagta tgcggatttt ttccgccagg tcgagtcttc accgctgaat
     3301 ccggcgcagg cccgggcagt cgttaatggc gagcattctc tgttagtgct ggctggtgca
     3361 ggaagcggaa aaacgtcggt gctggtggcc cgtgcaggct ggttgctggc gcgtggtgaa
     3421 gcgtcccctg agcaaatttt attgctggcg tttggtcgca aagccgctga agagatggac
     3481 gagcggattc gcgaacggct acataccgaa gacattaccg cacgcacgtt tcatgcgttg
     3541 gcgctgcata ttattcagca gggcagcaaa aaagttccga tagtcagcaa actggaaaat
     3601 gataccgctg cccgtcatga actctttatt gctgagtggc gcaagcaatg cagcgaaaag
     3661 aaagcgcagg cgaagggctg gcggcaatgg ctgacggaag aaatgcagtg gtcagtgcca
     3721 gaaggtaact tctgggatga tgaaaaatta cagcgtcgcc ttgcctctcg cctcgatcgc
     3781 tgggtaagtc tgatgcggat gcacggtggt gcacaggcag aaatgattgc cagtgcaccc
     3841 gaagagattc gcgatctgtt cagtaaacgt atcaagttga tggccccgtt attaaaagcc
     3901 tggaaaggtg cgctgaaggc agaaaacgct gtcgattttt cgggccttat tcatcaggcg
     3961 attgtgattc tggagaaagg ccgctttatc agcccgtgga agcatattct ggttgatgaa
     4021 tttcaggata tctcgccgca gcgcgcagcg ttgttagcgg cattacgcaa gcaaaacagt
     4081 cagacgacgt tgttcgccgt tggtgatgac tggcaggcga tttaccgatt cagcggtgcg
     4141 caaatgtcgc tcaccaccgc tttccatgaa aactttggtg aaggcgatcg ctgtgattta
     4201 gacacgactt accgttttaa cagtcgtatc ggtgaggtgg caaaccggtt tattcagcag
     4261 aacccaggcc agctgaaaaa gccgctaaac agcttaacca atggagacaa aaaagccgtc
     4321 acgttattgg atgagagtca acttgacgct ttgctggata agctctctgg ttatgccaaa
     4381 ccggaagagc gcattctgat cctggcgcgt taccatcaca tgaggcctgc cagcctggaa
     4441 aaagcggcaa cacgctggcc gaagttgcaa atcgacttta tgaccattca tgccagcaaa
     4501 gggcaacagg cggattacgt catcatcgtt ggcttgcagg aggggagtgg tggttttccg
     4561 gctgcggcgc gggagtcgat tatggaagag gcgctactgc caccggttga ggatttcccg
     4621 gacgctgaag aacggcgttt aatgtacgtg gcgctgaccc gggcacgcca tcgggtatgg
     4681 gcactgttta acaaagagaa tccctctccc tttgtggaaa tactgaaaaa tctggatgtg
     4741 ccggtggcga gaaaaccgta agaaacaggt ggcgtttgcc acctgtgcaa tattacttca
     4801 gacggtcagc gagataacgc tgataatcgg ggataagaat atcgaccgcg tcgttgaaat
     4861 gcggcgactg gataatgaag tctgccgttg ccacgttggt ggcgaccgga atgttccata
     4921 ccgtcgccag acgcagcaag gctttcacgt caggatcgtg cggcacggca tttagtggat
     4981 cccagaagaa tatcaataca tcaattttcc cttctgagat caatgcgcca acctgctggt
     5041 caccccccat tgggccactc aacatcgcgt tgacgttcat gccggtcgcg cgggaaatca
     5101 agttaccggt agtgcctgtt gcatacagta cgtgttgttc cagtaacggt tgatgccgtt
     5161 ccacccagct catcagcatt tgtttgcagt gatcgtgtgc caccagcgca atatgtttcc
     5221 gcgcaggtaa agtgcgagtc gtcagttcca taatgtacat ccgtagttaa ctttcctaca
     5281 gattactgta agcacttatc gctgcaagat aaagaccgaa aaagcctgcg cacaggcaca
     5341 aaaatctcag gaagatggtt gtttttccgc ccattgcagg aaagtatttc gcgtttgtgg
     5401 gtcagccagt ttaaaccaat acttcagccg ttgttctgtg agcacctgag actgcggtgg
     5461 aatagcatcc agctcggaaa cacccgaaag cagcgtactg tcatctgcca tcatcgtggc
     5521 aaattgcggt agtgaagcgc gtttcgcgga tttgttatag cgttcaacct cgacctcata
     5581 atcaatcgtt tttgccgttg aggtaatggc gagaatatcc agctttaccg gaatcggcgt
     5641 cgcatcgcca tccaacaatt caaggcgcgg cgcggcatca aaatggttag cttcccgctc
     5701 attctccagg cgaggcaggc gaaaattgac ctggttgatg agctgggtat taaaactgac
     5761 caccagcggt ggggagatat acagccgttc ttcactattg gaaagatgaa tcgtcttctc
     5821 aacgcgaaac actaactgat gcggtccatt gtccagttca atgctgtccg caccgcgcag
     5881 cagagagctg gaaaccttct tgccgtcgag taccagaaga tcgacatcgg ttgaaagccg
     5941 cagcgtggtg gcaaaaacgg ataccggcaa acataatgca atcaaggtgg tcacgatgcc
     6001 ggttttcata ggtagctcct gtcaaaagac cgcccgaata ataatgtgtc aaaatttttc
     6061 tttaatcata cttactaaca tatagacata tttcccgctt ttgctctcat tcattcgtat
     6121 tagctgcatg gttggcatgt cggctttgtc gtacactttg ttaaacagtc tggaggaaat
     6181 aatgaaagaa accgatattg caggcatttt aacgtccact cacaccattg cgctggtggg
     6241 ggcaagcgac aaacccgatc gccccagcta tcgcgtgatg aaatatctac tcgaccaggg
     6301 gtatcacgtc attccggttt cgccaaaagt tgccggcaaa acgctgctgg gccagcaggg
     6361 gtatggcacg ctggcagacg ttccggaaaa ggtcgatatg gtcgatgttt tccgtaattc
     6421 agaagcggcg tggggcgtgg cacaggaagc tattgccatt ggcgcgaaaa ccctctggat
     6481 gcaactgggg gtgattaatg agcaagcggc agtactggca cgggatgccg ggttaaatgt
     6541 ggtgatggat cgctgcccgg ctattgagat ccctcgcctg ggtctggcca aataaaaaat
     6601 ccccggaagg caaaaacctt ccggggattt gttcagggat tagttacgca gacgcggggc
     6661 ctggagttgt ttgcggatgg tctgcgccag ctcatccatg gatggctgtt caggatgttc
     6721 atcctgcaat tcgctactta gctgggcttc agccaggtag gtatgaaccg gtaggccgtt
     6781 atcgtcctcc atcaccacgt gataccacgg agcggcgcga agctcgtcat tcaccgccag
     6841 ctcatcgggc gacggttcag agagcgaata aaccgggtcg atatccacga ccactccgag
     6901 ataacctaac agggaatggc ggacctgctg gccgataccg aatttgctgg caatcatagt
     6961 cacctcccgg gaaatcttta cctttgatat aagggtaaaa ttccacattt caagttacat
     7021 gacgcgacag gcaaaccctt tcagatatag cccttccggg taggtagcga tcaccggatg
     7081 atcggctgcc tgacggaact gctctataaa ttgtacatca cgaccggcat caattgcggc
     7141 atccgcgatg attttctgaa ataaatcgct ggtcatcaga ccggaacagg agaaagtcag
     7201 gagaataccg ccttcattca gcaactgaat cgccagcatg ttgatgtctt tataaccacg
     7261 acacgcgccc atcaactggc ttttattctc aacaaacttc ggcgggtcca tcacgataac
     7321 gtcaaatttt tcaccgcgat cgcgataagt acgcagcaat ttaaagacat catcacggac
     7381 aaactcagcc ttgctcagat ccagtttgtt cagctcaacg ttctgccgtg caatatccag
     7441 cgcttcctgg gaggtatcaa cgctgacaac ctggctgcaa ccgcccatca gtgccgatac
     7501 ggcgaaacca ccggtatagg agaaacagtt cagcacacgt ttattttcaa cgtagcggcg
     7561 ggtagccagg cggctgtctc gctggtccag atagtagccc gttttgtgtc cgtgctgaat
     7621 atccaccagc agcttcatgc cgtgttcttc aatcggcagc agggcaggcg gcaactcgcc
     7681 ggtgacgagg ccctgggtca gctccatccc ttctttttta cgtaccgcaa catcgctgcg
     7741 atcgtaaatc gcacattccg ggtacagcgt ttgcagggca ctaattaatg ccgcgcgctg
     7801 atattctgcg ccagcactca gcagttgcag caccagaaaa ttaccgaaac gatcgatagt
     7861 aatacccggc aggccatcag attctccggc gattaaacga tagctgtcga ggccatcttt
     7921 ttgcgccagc cagtcacgcc atttttgtgc ttgttgcaaa cggcgggaaa aaaaagcaat
     7981 gtcgatagac tcagacgggt caaacgtcca gacgcgcgcc cggatttgcg aagctggcga
     8041 ataagcgccg cgtgctaacc attttccctg atgatcaaca atatcgatgg tttcaccgag
     8101 gctggcttta ccttccatgc gggcaacggc cccggaaaag acccacggat ggcgacgaag
     8161 taatgatttt tcgcgccctt tggctaacac taaacgtaca ctcataattt acttttctgt
     8221 cgatgcaaag aaatgggcgt cattgtccgg agttttccat ggatttgcaa cgcgtccatt
     8281 aaggataagg agagcagcaa tgtcgaaaat ctgcataatt gcctggattt acgggcgagt
     8341 tcagggcgta ggatttcgct acaccacaca gtacgaagcg aaaagactgg gcttaaccgg
     8401 gtacgccaaa aatcttgacg atggcagcgt ggaagtggtt gcctgcggtg aagaagggca
     8461 ggtagaaaaa ttaatgcagt ggttaaagag tggcggcccg cgttctgcgc gtgtagaacg
     8521 ggtgctcagc gagccgcatc atccctcggg ggaattaacc gattttcgta tccgttaaat
     8581 acattttacc ggttttggca ggccagcaat tttggtggct tgcttagccg ggcctttcgg
     8641 gaacagtcgg tataaatagc ggctgttacc tttttcttca ccaaatttat tcgccatcgc
     8701 ttttaccagc atacgaatcg ccggagacgt attaaattcc aggtagaagt cacgtacaaa
     8761 acgcaccact tcccagtgtt ctggcgacag cgaaatccct tcgttctctg caatcaccac
     8821 tgccagcggt tcactccact ggctgctttc tttgagatag ccttcggtat ccgtttctat
     8881 ctctttacct tcgaagatca gcataattat tactacctta atcagactgg cggcagtgta
     8941 acaaaaaaca aagccccgca taaagcgagg ctatgaaagt gttagcgggt gagattaatc
     9001 gcggctggcg aagcccagaa tgctcagtag gctgacgaag atgttgtaca gcgaaacata
     9061 caggctaacc gtggcacgaa tatagttcgt ttcaccgcca tgaatgatgt tgctggtttc
     9121 aaacaagata gcgccagagg agatcagaat gaagaccgcg ctgatcgcca gatgcagagc
     9181 aggcagttgc aggaagatat tagcaatcat accaatcagt accaccacaa tacccgccat
     9241 cagcataccg ccgaggaacg acatatcttt gcgggtggtc agcacatatg cagagcagca
     9301 gaagaacact aacgccgttc cgcccagtgc catagcgatt acgtcaccca ttccggcaga
     9361 cagataggtg ttcagaattg gtccgaggat ataacccaga aaaccggtaa aggcgaatgc
     9421 ggagataatc ccggtcggct tattcgccgt tttataggtc aggaacatca aaccatacat
     9481 acccaccaga gtcagaatca gacccggaga tggcagcatc agcaccgtac tggcagttgc
     9541 ggtaatcgcc gaaaaggcca gcgtcaggct cagcagaaaa taggtattac gcagcacctt
     9601 atgggtgcta agcagtgatg tacggtcatg tgaagaacta acaatacgat ccattgagtc
     9661 actctctatg acagatgtaa ttaattaagc agcataatga taatgcgtaa gggcacccag
     9721 aagttttacc catctttacg catttgatct ggaacaggtt taacagcgga ttatcaggtc
     9781 attaagcaaa tataacgccc tgagaattcc gacaggcaaa agaaaaaggg ggtagcgttt
     9841 agctaacccc ttatcttatt tggcggaagc gcagagattc gaactctgga accctttcgg
     9901 gtcgccggtt ttcaagaccg gtgccttcaa ccgctcggcc acacttccgg aatgacgcgc
     9961 actataaaca tcccgatgcg gcgtgtaaac ccctaatttg tttgtttgcc tgaaaaacag
    10021 ccaaaagtgc attgatagcg tgaaataaca gcagattgat catttcatca ccatgaattc
    10081 cttctctttt actcgtttag caaccggcta aacatcccca tcgcccggct aaagaaaaat
    10141 aggtccattt ttatcgctaa aagataaatc cacacagttt gtattgtttt gtgcaaaagt
    10201 ttcactacgc tttattaaca atactttctg gcgacgtgtg ccagtgcaga aggatgagct
    10261 ttcgttttca gcatctcacg tgaagcgatg gtttgccttg ctacagggac gtcgcttgcc
    10321 gaccataagc gcccggtgtc ctgccggtgt tgctaggaga agagacgtgc gatatgaata
    10381 acgaggaaac attttaccag gccatgcggc gtcagggcgt tacccggcgc agctttctca
    10441 aatattgtag tctggctgcc acgtcgctgg gattaggcgc gggaatggca ccaaagattg
    10501 cctgggcgct ggagaacaaa ccgcgcattc cggtggtatg gatccacggt ctggaatgta
    10561 cctgctgtac cgaatctttt atccgctccg ctcacccact ggcgaaggac gtcatccttt
    10621 ccctgatttc cctcgattac gacgataccc tgatggctgc cgccggaacc caggcggaag
    10681 aagtctttga agacatcatc acgcaatata atggcaaata tatcctcgca gtagaaggta
    10741 atccgccgct gggcgagcag gggatgttct gtatcagcag cggtcgaccg tttattgaga
    10801 aactcaaacg tgccgctgcc ggagccagcg cgattatcgc ctggggaacc tgcgcgtcct
    10861 ggggctgcgt gcaggccgcg cgacctaatc cgacacaggc aacgcctatc gacaaagtca
    10921 tcaccgacaa acccattatc aaagtacctg gctgcccgcc gatcccggat gtgatgagcg
    10981 ccatcattac ttacatggtg acctttgatc gcttgccaga tgtcgacaga atgggtcgtc
    11041 cactgatgtt ttatggtcag cgtatccacg ataaatgcta tcgccgcgct cacttcgacg
    11101 ccggagagtt cgtccagagt tgggatgatg acgctgcccg caaaggttat tgcctgtaca
    11161 aaatgggctg caaagggcct accacctata acgcctgttc ctccacacgc tggaatgatg
    11221 gcgtttcttt cccaatccag tctggtcacg gctgcctggg ctgtgcggaa aatggtttct
    11281 gggatcgcgg ttcgttctac agccgcgtgg tcgatattcc gcaaatgggt actcattcca
    11341 ccgccgatac cgtcggttta accgcgcttg gcgtggtggc agcagctgtt ggtgtgcacg
    11401 cagttgccag cgccgttgac caacgcagac gtcataacca gcaacctaca gaaaccgaac
    11461 atcagccagg caatgaggat aaacaggcat gagcactcag tacgaaactc agggatacac
    11521 catcaataat gccggacgcc gcctggtggt cgacccgatt acgcgcatcg aaggccacat
    11581 gcgctgtgaa gtgaatatta acgatcagaa tgtgatcacc aatgccgtct cctgcggcac
    11641 catgtttcgc gggctggaga tcatcctgca agggcgcgac ccgcgcgatg cgtgggcgtt
    11701 cgttgaacgt atctgcggcg tctgtactgg cgtacacgcc ctggcttcgg tttacgccat
    11761 cgaagatgcc atcggtatta aagtgccgga caacgccaat atcatccgca acattatgct
    11821 ggcaacgctc tggtgccacg atcatctggt gcacttctat cagcttgccg ggatggactg
    11881 gatcgacgtg ttagatgcgc tgaaagccga cccgcgaaaa acctccgaac tggcgcaaag
    11941 tctctcctct tggccgaaat catcccctgg ctatttcttc gacgtacaaa atcgcctgaa
    12001 gaaattcgtt gaaggcgggc agttggggat cttccgcaat ggctactggg ggcacccgca
    12061 gtacaaactg ccgccagaag ccaacctgat gggctttgcc cactatctcg aagctctcga
    12121 tttccaacgt gaaattgtca aaattcacgc ggtctttggc ggtaaaaacc cgcatccaaa
    12181 ctggattgtc ggtgggatgc cttgcgccat taacattgac gaaagcggcg cggtcggggc
    12241 ggtcaatatg gaacgcctga acctggtgca gtcgattatc acccgcacgg cagacttcat
    12301 taacaacgtg atgatccccg acgccttagc catcggtcag ttcaacaaac cgtggagcga
    12361 aatcggcacg ggtctttctg ataaatgtgt cctcagctac ggcgcattcc cggatattgc
    12421 caacgacttt ggtgagaaaa gtctgctgat gcctggcggc gcggtgatta acggcgactt
    12481 caacaatgtg ctgccagtgg atttggttga tccgcagcag gtgcaggagt ttgtcgatca
    12541 cgcctggtat cgttatccca acgatcaggt cgggcgtcat ccattcgatg gcattaccga
    12601 cccgtggtac aaccccggcg atgtcaaagg cagcgatacc aacattcagc agctgaatga
    12661 acaggaacgc tactcgtgga tcaaagcgcc acgctggcgc ggtaacgcga tggaagtggg
    12721 gccgctggca cgcacgttaa tcgcttatca caaaggcgat gctgcgaccg ttgagtcggt
    12781 cgatcgcatg atgtcggcgt tgaacctgcc gctttccggt atccagtcaa cgttgggccg
    12841 cattttgtgc cgcgcgcacg aagcgcagtg ggccgcaggt aagttgcagt atttcttcga
    12901 caagctgatg accaacctga aaaacggcaa tctcgccact gcttccactg aaaaatggga
    12961 acctgcaacc tggccgacag agtgccgtgg cgtcggcttt accgaagcgc cgcgcggagc
    13021 gttaggccac tgggccgcca ttcgcgatgg caagattgat ctctaccagt gcgtggtgcc
    13081 gaccacctgg aacgccagtc cgcgcgatcc caaagggcag attggcgctt atgaagcggc
    13141 gctgatgaac accaaaatgg cgatccccga gcaaccgctg gagatactgc gtactctgca
    13201 cagctttgat ccgtgcctcg cctgttcaac tcatgtgctg ggcgacgacg gtagcgagct
    13261 gatctccgtg caggtgcgtt aacagcgaag gagaatcatc atgcaacaga aaagcgacaa
    13321 cgttgtcagc cactatgtct ttgaagcacc agttcgtatc tggcactggc tgacggtgtt
    13381 atgcatggcg gtgttgatgg tcaccggtta ctttatcggc aagccgctac cttccgtcag
    13441 tggcgaggcg acgtatctgt tctatatggg ttacatcagg ttaattcact tcagcgccgg
    13501 gatgattttt accgtggtct tgctgatgcg gatctactgg gcttttgttg gcaatcgtta
    13561 ctcccgcgag ctgtttatcg tgccggtatg gcgtaaaagc tggtggcagg gcgtgtggta
    13621 tgaaatccgc tggtatctgt ttctggcaaa acgtccgagt gccgatatag gccataaccc
    13681 catcgcccag gcggcgatgt tcggctattt cctgatgtcg gtctttatga tcatcaccgg
    13741 ttttgcgctg tacagcgaac acagccagta cgctattttt gcgccgttcc gttatgtggt
    13801 ggaatttttc tactggacgg gtggcaactc aatggacatt cacagctggc atcggctggg
    13861 gatgtggctg attggggcgt ttgtgatcgg tcatgtctac atggcgctgc gtgaagacat
    13921 catgtccgac gacacggtga tctccaccat ggtcaacggc taccgtagcc acaaatttgg
    13981 caaaataagt aacaaggagc gttcatgagc gagcaacacg tggtggtcat ggggctgggc
    14041 aacctgctgt gggccgatga aggcttcggc gtgcgggtgg cggaacggct gtatgcccat
    14101 taccactggc ccgagtatgt ggagattgtc gatggcggta ctcagggact gaacttgctg
    14161 gggtatgtcg aaagcgccag ccatctgttg attctcgatg ccattgacta cgggctggaa
    14221 cctggaacgc tgcgaaccta tgccggagaa cgcattccgg cctatctcag cgcgaagaaa
    14281 atgagcctgc atcagaacag tttctccgaa gtgctggcgc tggcagatat ccgcggacat
    14341 ctgccagcac atattgccct cgtcgggctg caacccgcga tgctcgacga ctacggcggc
    14401 agcctgagcg aactggcacg ggagcaactg cccgctgcgg aacaatcggc gctggcgcag
    14461 ttggctgcgt ggggaagtgt gccgcaaccg gctaatgaat cgcgttgtct caattatgac
    14521 tgtctgtcga tggaaaatta cgaaggcgtt cgcttgcgcc agtaccggat gacacaggag
    14581 gagcagggat gagcaacgac acgccatttg atgcgttgtg gcaacgaatg ctggcgcgcg
    14641 gctggacgcc ggtcagtgaa tcccgtcttg acgactggct tacgcaagcg ccagacggcg
    14701 tggtgttatt aagcagtgac ccgaaacgca cgccagaggt cagcgataat ccggtaatga
    14761 ttggcgaatt actgcacgag tttcccgact atacatggca ggtggcgatt gctgaccttg
    14821 agcagagcga agccatcggc gatcgctttg gcgcctttcg ctttcctgcc actttagtgt
    14881 ttaccggcgg aaactatcgc ggcgtgctga atggtattca cccgtgggcg gaactgataa
    14941 acctgatgcg cgggcttgtc gaaccgcagc aggagcgtgc ctcatgagcg aaactttttt
    15001 ccatctgctg gggccaggaa cgcaaccgaa cgatgacagt ttcagcatga atccactgcc
    15061 gatcacctgt caggtgaatg atgaaccgag tatggcggcc ttggagcaat gtgctcacag
    15121 cccgcaggtg attgcgctgt taaacgagtt acaacatcaa ctaagcgaac gccagccgcc
    15181 gttgggcgag gtgctggcag tcgatctgtt aaatctcaac gccgacgatc gtcactttat
    15241 caatacgctt ctcggggaag gggaagtgtc agtgcgcatt cagcaggctg acgacagtaa
    15301 aagtgaaata caggaggcga tcttctgcgg attatggcgg gtgcgcagac gtcgcggcga
    15361 aaagttgctg gaggacaaac tggaggctgg ctgcgcgccg ctggcgttgt ggcaggcggc
    15421 aacgcaaaat ctcttgccga cagattcgct gttaccgccg cccattgatg gcctgatgaa
    15481 tggcctaccg ttggcgcatg agttactggc acatgtacgt aaccccgacg cgcagccgca
    15541 cagcattaat ctgacgcaat tacccatcag cgaggctgat cggctttttc tctcgcgtct
    15601 ctgtgggccg ggaaatattc agattcgtac cattggctat ggcgagagct atatcaacgc
    15661 cacggggtta cgccatgtct ggcatttacg ctgtacggac accttaaaag gcccgttact
    15721 ggaaagttat gaaatctgcc caataccgga agtggtgctg gcagcgccag aagatttggt
    15781 cgactctgcg cagcggctta gcgaggtatg tcagtggctg gcggaagctg caccgacgta
    15841 aaaagacggt aagtatcgct ttcagtctta tgaatatcgc aatcggcgaa tacctctggt
    15901 cgtagagttt caggataaag agggagatct aacattatcg ggttattttt ctctcttcgc
    15961 ctacaggagt gcgcatgtgg gatgtcattg atttatcgcg ctggcagttt gctctgaccg
    16021 cgctgtatca ctttttattt gtacccctta ccctggggct gatttttttg ctggctatca
    16081 tggaaaccat ttacgtggtt accggcaaaa caatctaccg cgatatgacg cgcttctggg
    16141 gtaagctctt cggtatcaat tttgctcttg gcgtggctac cggcctgacc atggagtttc
    16201 agtttggtac taactggtca ttctattcca actatgtggg cgatattttt ggcgcaccac
    16261 tggcgatgga agcattaatg gccttcttcc tcgaatccac ctttgtcggg ctgttcttct
    16321 tcggctggca acggctgaat aaataccagc acctgctggt gacgtggctg gtggcgttcg
    16381 gttcaaatct ctctgcattg tggatattga atgccaacgg ttggatgcaa tacccgaccg
    16441 gtgcgcattt tgatatcgac accctgcgta tggagatgac cagcttcagc gagctggtct
    16501 ttaacccggt cagccaggtg aaatttgtgc acaccgtcat ggcgggctac gtgaccgggg
    16561 ccatgtttat tatggcgatc agcgcctggt atttactgcg cggacgggag cgcgatgtcg
    16621 cattacgctc gtttgccatc ggttccgtct tcggtactct ggcgattatc ggtaccctgc
    16681 aactcggaga cagttctgcg tatgaagtcg cgcaagtaca accggtaaaa ctggcggcga
    16741 tggaagggga gtggcaaacg gaacctgctc ctgcaccgtt ccatgtggtt gcctggccgg
    16801 aacaggatca agagcgtaac gcctttgcca tcaaaattcc cgcgctgcta gggatcctcg
    16861 ccactcactc attagataaa cccgtgccgg gtctgaagaa tttgatggct gaaacctacc
    16921 cacgcttgca acgcggacgt atggcctggc tgttaatgca ggaaatatct caaggcaatc
    16981 gtgagccgca tgtgttgcag gcattccggg agctggaagg cgaccttggt tacggcatgt
    17041 tgctctcccg ctatgcgccg gatatgaatc atgtcacagc cgcacagtac caggcggcga
    17101 tgcgtggcgc gatacctcag gttgcgccgg tattctggag tttccgcatc atggtgggct
    17161 gtggttccct gctgctactg gtgatgctga ttgcgcttgt ccagacgctg cgtggcaaaa
    17221 tcgaccagca tcgctgggtg ctgaaaatgg cgctctggag tttgccgttg ccgtggattg
    17281 cgattgaagc cgggtggttt atgaccgagt ttggtcgtca gccgtgggcg atacaggaca
    17341 tcttaccgac atactccgcg cactccgctt taaccacagg acaactggct ttctcactga
    17401 tcatgatcgt agggctttac accctgttct taatcgccga agtctacctg atgcagaaat
    17461 atgcccgtct ggggccgagc gcgatgcaga gtgaacaacc gacgcagcaa caggggtaaa
    17521 ggagaaaatc atgtttgatt atgaaacatt gcgcttcatc tggtggctgc tgattggcgt
    17581 gatcctggtg gtctttatga tctccgacgg atttgacatg gggatcggct gtttgctgcc
    17641 gctggtggcg cgtaatgatg atgaacgccg gatagtgatt aatagcgttg gtgcgcactg
    17701 ggaaggcaat caggtctggt tgatcctcgc aggtggggca ttatttgcag cctggcccag
    17761 agtgtatgca gcggcgtttt ccggctttta tgtggcgatg atcctggtgc tgtgctcgct
    17821 gttcttccgt ccgctggcct ttgattatcg cggaaaaatt gccgatgcac gctggcgtaa
    17881 aatgtgggat gccggtctgg tcatcggcag tctggtgccg ccggtagtct tcggtatcgc
    17941 cttcggcaac ttgttgctcg gcgtgccgtt tgccttcaca ccgcaattac gcgtggagta
    18001 tctcggcagc ttctggcaac tgctgacgcc attcccttta ttgtgtggtt tgctcagcct
    18061 tgggatggtg attttgcaag gtggcgtctg gttacaactg aaaactgttg gtgtgattca
    18121 tctgcgttca cagctggcga ctaaacgcgc tgcactgttg gtgatgctgt gctttttgct
    18181 ggcaggttac tggctgtggg tcggtattga tggctttgta ctgctcgccc aggatgctaa
    18241 cggtccttcc catccgttaa tgaaactggt ggcagtgcta cctggtgcct ggatgaataa
    18301 tttcgtcgaa tcgcccgttt tgtggatctt cccgctgctg gggttcttct gcccattgct
    18361 gacggtgatg gcgatttatc gtggtcgccc gggttgggga tttttgatgg catcattgat
    18421 gcaatttggc gtgattttca cggcaggcat cacgctgttc ccctttgtca tgccgtcaag
    18481 tgtgagtcca atctccagcc tgacgttgtg ggacagcact tccagtcagc tgacgctgag
    18541 cattatgttg gtaatcgtgc tgatattttt gcccattgtg ttgctctaca ctctctggag
    18601 ctactacaaa atgtggggac gcatgacaac agaaactctc cgccgtaaca aaaacgagtt
    18661 gtactaagga gcaggaacat gtggtattta ctttggttcg tcggcatttt gttgatgtgt
    18721 tcgctctcca cccttgtgtt ggtatggctg gacccgcgtc tgaaaagtta acgaacgtag
    18781 gcctgatgcg gcgcattagc atcgcatcag gcaatcaata atgttagata tgaaaagcgg
    18841 aaacatatcg atgaaagcga tcttaatccc atttttatct cttctgattc cgttaacccc
    18901 gcaatctgca ttcgctcaga gtgagccgga gctgaagctg gaaagtatgg tgattgtcag
    18961 tcgtcatggt gtgcgtgctc caaccaaggc cacgcaactg atgcaggatg tcaccccaga
    19021 cgcatggcca acctggccgg taaaactggg ttggctgaca ccgcgcggtg gtgagctaat
    19081 cgcctatctc ggacattacc aacgccagcg tctggtagcc gacggattgc tggcgaaaaa
    19141 gggctgcccg cagtctgctc aggtcgcgat tattgctgat gtcgacgagc gtacccgtaa
    19201 aacaggcgaa gccttcgccg ccgggctggc acctgactgt gcaataaccg tacataccca
    19261 ggcagatacg tccagtcccg atccgttatt taatcctcta aaaactggtg tttgccaact
    19321 ggataacgcg aacgtgactg acgcgatcct ctgcagggca ggagggtcaa ttgctgactt
    19381 taccgggcat cggcaaacgg tgtttcgcga actggaacgg gtgcttaatt tcccgcaatc
    19441 aaacttgtgc cttaaccgtg agaaacagga cgaaagctgt tcattaacgc aggcattacc
    19501 atcggaactc aaggtgagcg ccgacaatgt ctcattaacc ggtgcggtaa gcctcgcatc
    19561 aatgctgacg gagatatttc tcctgcaaca ggcacaagga atgccggagc cggggtgggg
    19621 aaggatcacc gattcacacc agtggaacac cttgctaagt ttgcataacg cgcaatttta
    19681 tttactacaa cgcacgccgg aggttgcccg cagtcgcgcc accccgttat tggatttgat
    19741 catggcagcg ttgacgcccc atccaccgca aaaacaggcg tatggtgtga cattacccac
    19801 ttcagtgctg tttattgccg gacacgatac taatctggca aatctcggcg gcgcactgga
    19861 gctcaactgg acgcttccag gtcagccgga taacacgccg ccaggtggtg aactggtgtt
    19921 tgaacgctgg cgtcggctaa gcgataacag ccagtggatt caggtttcgc tggtcttcca
    19981 gactttacag cagatgcgtg ataaaacgcc gctgtcatta aatacgccgc ccggagaggt
    20041 gaaactgacc ctggcaggat gtgaagagcg aaatgcgcag ggcatgtgtt cgttggcagg
    20101 ttttacgcaa atcgtgaatg aagcacgcat accggcgtgc agtttgtaat gcataaaaaa
    20161 gagcattcag ttacctgaat gctctgaggc tgatgacaaa cgaagaactg tctaatgcgt
    20221 agaccggaaa aggcgttcac gccgcatccg gtcactttca gttttactct ttctcggagt
    20281 aactataacc gtaatagtta tagccgtaac tgtaagcggt gctggcgcgt ttaatcacac
    20341 cattgaggat tgcgccttta atattaacgc cagcctgttc cagacgctgc attgacaagc
    20401 tcacctcttt ggcggtgttc aagccaaaac gcgcaaccag caggctggtg ccgacagagc
    20461 gtcccacgac cgcagcattg ctcaccgcca gcatcggcgg cgtatcgaca atcaccagat
    20521 cgtaatggtc gttcgcccat tccagtaatt gacgcatccg atcgcgcatc agcagttcag
    20581 acgggttagg tggcacctga ccgcgagtaa tcacatcaaa gtctcctttg ccaaaatgct
    20641 ggatcacttt gttgagctca tctttacctg ccagatattc cgacaagcca tgttcattac
    20701 tcacggtaaa caggttatgc gaataaccac ggcgtaagtc ggcatcaata aataacactt
    20761 tttgatcgga ctgggcgatc accgctgcca gagttgaact gacaaacgtt ttaccactgt
    20821 ctggcgtcgc accggtgatc atcagaatgt tattctcagt ctccatcata gcgaaatgca
    20881 gactggttcg tagcgcacgt acggcttcca cagcagaatc cgccgggtta tccaccgcca
    20941 ggaaggggat atttttagta cgatggcgct gctgattaga aaataaattt ttcttacgca
    21001 gacgggtgcg tttatccagc cactcggaca ttgggatagt ggaataaacg ctgatgccgt
    21061 gctcttccag ttgttccggg gcttctacac cacgacgcaa catcgcacgc gccagcacgg
    21121 cactcaccga aataaacagg ccaagaataa aaccaagcac cacattcaac gcttttttcg
    21181 gtttcactgg ctgcggctga gtgactgccg ggtcgataat ccgcacgtta ccaatggcac
    21241 tggatttcga aatactcaac tcctgctggc ggttaagtaa ttgcagatat accgcacggc
    21301 ccgcttctac gtcacgactt aaacgcaaca cttcctgttg ggtggaaggc attgccgata
    21361 cccgcttatt caggcgtttg cgttcttgct ccagcgtctg gcgtttttcc agcagcgcac
    21421 gataagttgg gtgatctttc ttatacagct gggagatctc tgcctcgcgg aaggtcagct
    21481 cattgagttg attatcaacg ttcacaatct gctcaagaac ggctttggct tccaggttaa
    21541 ggtcaaccga atcgcgctgc tggcgataaa cgttgagttt ttcttccgcc tggtccagct
    21601 cgctgcgcac ttcaggtaac tggcgctgta agaattcaag gctttgtgaa tcctgcgccg
    21661 cctggcgagc gatattctgt tgcaaatagt tgttagcgat gctgttcaga atacgagtaa
    21721 tcaactgggg atcatcacca gtcatggtaa gttccagcat cccgctttct ttactgcgtt
    21781 cgctaacggt aaaggtttcc tgcaatgcgt taatcgcttc cagttcggta cgctggctca
    21841 ggacaaactg tgttcctggt ttggccttaa tgtccgcgat agtcagcgca acgccatctt
    21901 tttccagacg ctggccgacc ataccattga cggtgaactc ttcaccttcc agtgtatagt
    21961 ggccgttttc cccaaccgtg agtgtcagtt gctgatcctg accattcagt tgtggaatat
    22021 gcatccagct gatcgccagc tcacctagtt tttctttggt taatctcgcc cagccgcgac
    22081 ccacaatcgg aaaatacttc tgctcaacta tgtcgcgcag attcagttca gcaatggttt
    22141 taccgagaat catgcgcgat tgcagcagtt ggatctccgg tgcagactcg ggcgatgagt
    22201 tagggatcat atcgctcagg ccgctgagaa tggcgttgcc ctgtttttgc tcaacctgga
    22261 ccagagtatc tgcctgataa attggtgtgc ttaacagcga tcagcgtgaa taacgcggtc
    22321 acgctgataa taaacttacg gtgatcccat aactcgccga ccagacgaag cagatcgatc
    22381 tcattttcct gagtgctgcc tggtggcgta ttcatatttt tagttgtcat cgtattttat
    22441 aaactcaaat taccggctga ggcgcttcgc ccattcctga ctggcgcgct ccaacatact
    22501 gtagacatgt tcaaatgcgt cctgactttt acgataggga tccgggatct ctttctgttc
    22561 cagccattgc ccaaacagca ttgtcttccc gcggacctcg ggagcaattg cggtaacctg
    22621 agcaatatgt tccgactcca ttgccagaat cagatcgtaa tttcgtgcca tctcagcagt
    22681 gagcttacgt ccggcatgtc cttcaagaga tacgccatga ttagcggcaa catctgccgc
    22741 tgtcgcatct gcagggtgtt ttaccagacc atgaacacct gccgatttga cttttacgcc
    22801 cggcagacgt ttacgcagca agcgctcgcc aatgggcgaa cggcaaatgt tgccggtaca
    22861 aaccactagg attgagttaa attttagttg ggccatgttt gaatccaccg tgaagtctct
    22921 gtcaggtcat gcaccccgga aatcgtcggt accagcagag aaatcacacg gttccaacga
    22981 gccagcggcg cggtagtgac ataaacaatg tcgtaaggtt caagctggaa ttccgttccg
    23041 agtatcattg ccgatgcgtc cttcgcattc agctgataaa tattggcgat tttgccattc
    23101 tgcttgtttt gcgttgcacg aataacaaaa atccccgtcg catcagcgac atcctgattc
    23161 ataccctcag cgttacccag cgcttctgcc agggtcatac cgctgcggtc cattttcagc
    23221 gtgctctgtt tacccacttc acccatcacg aagactttca gatcatcgtt gcgcggaata
    23281 aataaaatat cgcccgggtg cagcagtttg ttctggcgca agtcgccacg ctgcatcagc
    23341 gcatagaggt tcactttggt ctttacgcca ttctgggtga gcaccacgtt gcgccagtct
    23401 gcatctgcgg tcagaccgcc cgcggcgtta atcgcatcca tgatggtcag aggaatattg
    23461 gtaatcggct gctgacccga tttgctgact tcgccggtga cgtacgcttt ttgcgatcgg
    23521 aacgccgcga cgctgacatc gacttgcggg ctttcaatga ctgaatcgag acgcgctgta
    23581 atctcgttgc gtacctgggt aagtgttttc ccggccactt tcaggcgacc aatatagggg
    23641 tagaaaatcg caccatcggc gttaacccag ttgccggtat cactggcgct acggtattgc
    23701 cctgcggggg tggttagttc cgggtgatcc cagaccgtaa ccatcaacac atcaccaatc
    23761 ccgatacgat attcatagtt tgcgatttgc tgctccagct ccgggttagc ctgcgacata
    23821 atcggtttgg cgcgcagctg atcaatcaat cctggcgtga cgggatagat attgaccatt
    23881 ttgtccaggt catactgatt gtcaggtagt tcaatgacat ccttgttact ggtcgataaa
    23941 ttctgaccag gaacaagggt acatgctgtc aaagacagaa cagccaatgt cagtaccgaa
    24001 aacttaaaaa tgttcttttt cattggttta tatttatcag tcaatgtaat gttatgaatt
    24061 acgtcaggtt attgtccaac cggtacttct cgctctgccg tcattggatc gagctggtat
    24121 ttgcgtgaca gcatttgtcc accatcacgc gtcaatggtg tccacgaaac caccgcgcgg
    24181 ttgcggttag gtccaatggt catcaagtcg aacggaatcg agatataaaa acctttacta
    24241 aagccgcctt cgccgtagtc atctttagat acgttactga ttgccgccca tacccctacc
    24301 gccacgccgc tgtcaaagag tttggcgacg tcgatcgttg cgcctttatc ttttgccaga
    24361 tattgcccaa cgctaagttt catcagtacg ccattgagcg tcggcgggtt ccagtaagcc
    24421 gtcacgaaac ctgtgggcgt ggaataatcg gtgaagcgca tcatgttgtc ccagtcacgc
    24481 tgcttcacgt agttaacgtc cacacccagc gcccagctgg catctagcgg gcgataaaga
    24541 agctcggaac cgacaccggc atacatcgtt tccagataac cgccatacac ctggccatag
    24601 aaaccattgc ctaagtcggc aaagtaattc gcttgcaagt tgttgagata aacgtcattg
    24661 cgaacgtaat cacggatatg cgtgcgcacg cggggcaggg tagagtccgc gggtaacagt
    24721 gaagacttaa acttgtcgta gttgttgtaa atattggtga aaataccgtc atcaagcagc
    24781 aggtggtcgg taaaccagta gcgggcgctg gacatcagcc ccagctggaa catatagaaa
    24841 tcttccgggc cgcccagcga ctgtgaaagt gttgggttga aagagtagct aaagcgatct
    24901 tcacgaatac ggtagcaccg accaaaggca gaaagatctt ctgcttcaac acgttgttgt
    24961 tgcagttgct ctgatttacc aggcgctgtg ccggccagct gtttgcgcag gctggctacg
    25021 tcggtttcgg tagtcaccat cgccatatgc tcgcgctttt gcgtcacgct aatcttctca
    25081 acgccttgcg gcaggttatt caccagaatc cggttggcac gatcgaccgc ttcgcgagag
    25141 tcacggtatt tgtattgctg accagacata tacagcgtct tatcgcgtag ctgaatttct
    25201 ggcgcgtcaa agcccgcgtt atacttcagg gcggtaagtt ggtttgctac cgtggtgtac
    25261 tgcaaacctt cagactctgg cgcaggctgc caggccggtt ttggcgtatc gcgtagggca
    25321 gggcgcagat cgttgaaatt ggtccgtaaa gtgaaaccaa acatcaacgt gtttccgcgt
    25381 tcataactga ggttgaggtc tgcccagctg gcagcgcgat aaactgcacc gacgttgaaa
    25441 tggcttgcct gaggcagttt gccagcgaaa tcattctggt aattgttgcc gtcgtattcg
    25501 agtttcagac gcaggggatt ccacggcgtt tgatactcaa tgccgccaaa gatggaagcc
    25561 gggccacgaa agatatcgct aaagctgata tcgcccgcat cgtgagactc tgcgcgatga
    25621 caatatttat cgctcacccg gcaaaacggg ttggtaatat tgcccgcatt accggcgtag
    25681 ccccatgcca tcccgagggt gaaatcaaat ggccccgctt gcttgctggc caccagatat
    25741 tcaccgtcaa acaaacccgt accagcaata tcacgtttac caaacgccac ttgcggtagc
    25801 cagtaacctt cttcccacag gcgaagttta aaatcgaatg atttgtcttt gtatgactga
    25861 tcgccgctga aatcttccca ctggctatat ttgcgtgtgc gcacatccgt ataacgaatg
    25921 gtgccttcca gccatgggaa cagcgccacg gaggtggagt agaaccggta ttgatcgtta
    25981 tcccggtagt tgacgctgaa ttcaccttcc ggtgcgatgc gcgcatttgg catctgcaac
    26041 aatcctgtgc cgccaaagtc tgattgcgac gaacccagcg gatccgggta ggttaatact
    26101 tcagcatgac aggcactgga gacggcaatg gccaggcagc ttaaaagata agaattcttc
    26161 ttcatcagtc aggaacacgt tgcgttaaaa cagacacaat ctggtcattt aaatcagcat
    26221 atttttcggg cagtacatgc gcagaaaagc ccaaccaaag ctggctgcca ggcggtggtt
    26281 ccacatgtct tttattccac agggcaacag gggcgacgac agtctcaccc tcaggcgtaa
    26341 tcaccataac gttattcttg tctgcgcctg caaggcgggg gtgatcttgc agataatcgg
    26401 taacgctacg gcctgcctgc cagggcaatt gcccagcccc agaaaccgca cccagcagag
    26461 taatagtcac cggacgctgt acggtataga gcgtatagtc cccgaccaac gggggattgc
    26521 tgttctcatc cactcgtaca aaatcagggt ccagtttgac cggtaagcga ccagtgatat
    26581 tgagattgag taactgctgg cgcacggatt taatcgttgc cgccacatcg tcgtcagctt
    26641 ctgcttccca ggaagcaagc tgtgccatga catgttggta gtctttaagc gctttagctt
    26701 tcgccgcaga gtcggtcagc aaggcaccag gccaccagag gcgatcgcgc agttgcggtt
    26761 gtgtcaccaa ctggacaaca ttttccacgg ggccaacgga aagtgtctgt tgttcgctag
    26821 gcagataaat agtcaccgtt ccttgcgcaa aggcatgggg tgtcattacg taaagtacgc
    26881 tggcaatgaa atacgactgt aatttattca tggtgcgggt ttaagaaacg tcatttctac
    26941 gggaataacc cctgcgccta acatttgccg actttgacga acctggccag tagccgaatc
    27001 gacccagaat gtgttttgcc actgtttttc tggcagcgtg gaagtcacct cttcctgcca
    27061 gacattacac agcacattgt gacccgcaat attcagcgtc tctaatccag caaaggaaaa
    27121 agtagaactt agggtcgcag agcgaaaatg gttgtcttca ctccagagaa tatcgcgcgt
    27181 ccagcggctg ccatctttta ttgccagcgc gttacgcaga ggggcccgcc cgggatttgt
    27241 cacttccagc agattattat taagtttgac ggtctttaaa agacgtccat tgtgggtaac
    27301 cagcatggcg ttatcctggg ataaccattt gctttgttct tgttctatat atcccagtac
    27361 cacaaagatt cgttgccctt cattaaggcg taaatacatc gtggaataag gcaacgcctg
    27421 gatctgctga gccgctacag tgatatcctg attatcgaaa aggctggcgc gaaatgtatc
    27481 gaccatactt tgctggctgt gcgtacatcc cgcaagaaat agtgcgaaaa tcgataaaat
    27541 aagagggcgc acctttaatc cttgaaaggt taattaaaaa aatgcaaatc tctaaagccc
    27601 ggtacgaata tcgcaaccgg gtattattta acttgaattt cgacgcgttt aagcagaatt
    27661 agcgcgtagt ggacgtggtg gttgaggtgt tatgagaacc gccatcgcca ctgtcatttg
    27721 ctgcaactac tgccaacgtc gccacaccaa cagccgttgc cacaccgaca actgtcgccg
    27781 tgccgacagc gccagcagcc acgccactag tggtagccgc agtcgtggtt gcacctgtag
    27841 tggtcgttgt ggtgccagta gtacccgtcg ttgcctcggt cccgtttgct gcttcagggg
    27901 cagcaaacgc agctggcgtc gtcaacatga aggccataag gattgcagaa agcttgtgtt
    27961 tcataacttt tcctttattc atcgcatgga caatacgtac gcttgagatc aggattgtct
    28021 actttgagta taacatcctt gtgaaatctc cctttcctca taccgctagc actatatact
    28081 gcgggtggga aaggcgcgca gagggaaata taagattgtt aacgatttcc ctctggaaat
    28141 acgacttgtg agcataagat aaatcttaga aaaatacgaa ttaaagataa gaaaatt
//
LOCUS       DAZPCS010000035        27601 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZPCS010000035 DAZPCS010000000
VERSION     DAZPCS010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27601
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.035"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            319..1617
                     /gene="kgtP"
                     /locus_tag="ACHUQ7_001535"
     CDS             319..1617
                     /gene="kgtP"
                     /locus_tag="ACHUQ7_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFR1135005.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1614..1937)
                     /locus_tag="ACHUQ7_001536"
     CDS             complement(1614..1937)
                     /locus_tag="ACHUQ7_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFR1135006.1"
                     /translation="MRILFVCSLLLLSECSHMDNDSWSGQDKAQHFIASAILSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="ACHUQ7_001537"
     CDS             complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="ACHUQ7_001537"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFR1135007.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3452..6112)
                     /gene="pat"
                     /locus_tag="ACHUQ7_001538"
     CDS             complement(3452..6112)
                     /gene="pat"
                     /locus_tag="ACHUQ7_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFR1135008.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRNHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="ACHUQ7_001539"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="ACHUQ7_001539"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFR1135009.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="ACHUQ7_001540"
     CDS             complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="ACHUQ7_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFR1135010.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7537..8574
                     /locus_tag="ACHUQ7_001541"
     CDS             7537..8574
                     /locus_tag="ACHUQ7_001541"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFR1135011.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVLDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8622..9311)
                     /gene="ung"
                     /locus_tag="ACHUQ7_001542"
     CDS             complement(8622..9311)
                     /gene="ung"
                     /locus_tag="ACHUQ7_001542"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFR1135012.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAECE"
     gene            9616..9999
                     /gene="grcA"
                     /locus_tag="ACHUQ7_001543"
     CDS             9616..9999
                     /gene="grcA"
                     /locus_tag="ACHUQ7_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFR1135013.1"
                     /translation="MITGIQITKAANADLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="ACHUQ7_001544"
     CDS             complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="ACHUQ7_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFR1135014.1"
                     /translation="MTPILLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10745..11626
                     /locus_tag="ACHUQ7_001545"
     CDS             10745..11626
                     /locus_tag="ACHUQ7_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1135015.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPHFAVEKEIESGELIELPFGEQSQTITAMCAHYAGKAVS
                     PAMHIFIQCVEECFVVA"
     gene            complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="ACHUQ7_001546"
     CDS             complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="ACHUQ7_001546"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFR1135016.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13127..13864
                     /gene="trmN"
                     /locus_tag="ACHUQ7_001547"
     CDS             13127..13864
                     /gene="trmN"
                     /locus_tag="ACHUQ7_001547"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFR1135017.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="ACHUQ7_001548"
                     /pseudo
     CDS             complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="ACHUQ7_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
     gene            15727..15882
                     /gene="rseD"
                     /locus_tag="ACHUQ7_001549"
     CDS             15727..15882
                     /gene="rseD"
                     /locus_tag="ACHUQ7_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFR1135018.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15879..16454
                     /gene="rpoE"
                     /locus_tag="ACHUQ7_001550"
     CDS             15879..16454
                     /gene="rpoE"
                     /locus_tag="ACHUQ7_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFR1135019.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16487..17137
                     /gene="rseA"
                     /locus_tag="ACHUQ7_001551"
     CDS             16487..17137
                     /gene="rseA"
                     /locus_tag="ACHUQ7_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFR1135020.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17137..18093
                     /gene="rseB"
                     /locus_tag="ACHUQ7_001552"
     CDS             17137..18093
                     /gene="rseB"
                     /locus_tag="ACHUQ7_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFR1135021.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18090..18569
                     /gene="rseC"
                     /locus_tag="ACHUQ7_001553"
     CDS             18090..18569
                     /gene="rseC"
                     /locus_tag="ACHUQ7_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFR1135022.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18767..20566
                     /gene="lepA"
                     /locus_tag="ACHUQ7_001554"
     CDS             18767..20566
                     /gene="lepA"
                     /locus_tag="ACHUQ7_001554"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFR1135023.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20582..21556
                     /gene="lepB"
                     /locus_tag="ACHUQ7_001555"
     CDS             20582..21556
                     /gene="lepB"
                     /locus_tag="ACHUQ7_001555"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFR1135024.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21828..22508
                     /gene="rnc"
                     /locus_tag="ACHUQ7_001556"
     CDS             21828..22508
                     /gene="rnc"
                     /locus_tag="ACHUQ7_001556"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFR1135025.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22505..23410
                     /gene="era"
                     /locus_tag="ACHUQ7_001557"
     CDS             22505..23410
                     /gene="era"
                     /locus_tag="ACHUQ7_001557"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFR1135026.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQDKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGCDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23422..24150
                     /gene="recO"
                     /locus_tag="ACHUQ7_001558"
     CDS             23422..24150
                     /gene="recO"
                     /locus_tag="ACHUQ7_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFR1135027.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGDTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDG
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24162..24893
                     /gene="pdxJ"
                     /locus_tag="ACHUQ7_001559"
     CDS             24162..24893
                     /gene="pdxJ"
                     /locus_tag="ACHUQ7_001559"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFR1135028.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24893..25273
                     /gene="acpS"
                     /locus_tag="ACHUQ7_001560"
     CDS             24893..25273
                     /gene="acpS"
                     /locus_tag="ACHUQ7_001560"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFR1135029.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25693..25773
                     /gene="shoB"
                     /locus_tag="ACHUQ7_001561"
     CDS             25693..25773
                     /gene="shoB"
                     /locus_tag="ACHUQ7_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFR1135030.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="ACHUQ7_001562"
     CDS             complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="ACHUQ7_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFR1135031.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            26442..27538
                     /locus_tag="ACHUQ7_001563"
                     /pseudo
     CDS             26442..27538
                     /locus_tag="ACHUQ7_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
BASE COUNT         6806 a         6866 c         7236 g         6693 t
ORIGIN      
        1 taagcgaaag gccatccgtc aggatggcct ttttgcattg gcgcagaaaa aaatgcctga
       61 tgcgacgctg cgcgtcttat actcccacat atgacagatt cagcaacgga tgcggtttcc
      121 ccaacttgcc tacttccata cgtgtcctcc ttaccagaaa tttatcctta agctcctcaa
      181 taaccatttt cctgctaact aaattcatgg tttaggttgc ataatgatac gcaacaaatg
      241 tataacattt tctttaccaa aagaaataaa caaaagcgac caacaaaagc atcggattac
      301 ggcaggagac ataatggcat ggctgaaagt actgtaacgg cagacagcaa actgacaagt
      361 agtgatactc gtcgccgcat ttgggcgatt gtgggggcct cttcaggtaa tctggtcgag
      421 tggttcgatt tctatgtcta ctcgttctgt tcactctact tcgcccacat tttcttccct
      481 tccgggaaca cgacgactca actactacaa acagcaggtg tttttgctgc gggattcctg
      541 atgcgcccaa taggcggttg gctatttggc cgcatagccg ataaacatgg tcgcaaaaaa
      601 tcgatgctgt tatcggtgtg tatgatgtgt ttcggatcgc tggttatcgc ctgcctccca
      661 ggttatgaaa ctataggtac gtgggctccg gcattattgc ttctcgctcg tttatttcag
      721 ggattatccg ttggcggaga atatggcacc agcgccacct atatgagtga agttgccgtt
      781 gaagggcgca aaggttttta cgcatcattt cagtatgtga cgttgatcgg cggacaactg
      841 ctagccctac tggttgtcgt ggttttacaa cacaccatgg aagacgctgc actcagagag
      901 tggggatggc gtattccttt cgcgttagga gctgtgttag ctgttgtggc gttgtggtta
      961 cgtcgtcaat tagatgaaac ttcgcaacaa gaaacgcgcg ctttaaaaga agctggatct
     1021 ctgaaaggat tatggcgcaa tcgccgtgca ttcatcatgg ttctcggttt taccgctgcg
     1081 ggctcccttt gtttctatac cttcaccact tatatgcaga agtatctggt aaatactgcg
     1141 ggaatgcatg ccaacgtggc gagtggcatt atgactgccg cattgtttgt attcatgctt
     1201 attcaaccac tcattggcgc gctgtcggat aagattggtc gccgtacctc aatgttatgt
     1261 ttcggttcgc tggcagccat ttttaccgtt cctattctct cagcattgca aaacgtttcc
     1321 tcgccttatg ccgcttttgg tctggtgatg tgtgccctgc tgatagtgag tttttataca
     1381 tcaatcagtg gaatactgaa ggctgagatg ttcccagcac aggttcgcgc attaggcgtt
     1441 ggtctgtcat atgcggtcgc taatgctata tttggtggtt cggcggagta cgtagcgttg
     1501 tcgctgaaat caataggaat ggaaacagcc ttcttctggt atgtgacctt gatggccgtg
     1561 gtggcgtttc tggtttcttt gatgctacat cgcaaaggga aggggatgcg tctttagtga
     1621 cgggtcagtt gccagacggt atagccggtg cttgcaccgg cgacatccca ggccaaatcc
     1681 ttccagctcc agccgctccc ttcggggcgg ctatcccaaa gctcttttga cgcccccaaa
     1741 ctgacagaga acatcaatcc aaacatggca ctgcgatccc ggctcatccc ctgatgctgt
     1801 gaatattcat ttccggcggc ggaaagtatc gccgaggcga taaagtgttg agctttatcc
     1861 tgcccactcc agctatcgtt atccatatgg ctgcattcag aaagtaacaa tagcgaacag
     1921 acaaaaagaa tacgcaaaac gtggcctcca aaaaacaaac cccgtacagg acggggttgt
     1981 gattacagga tgcggctaat taatcggtcg atgcggatac ggcgcaaacg gcggatgagt
     2041 ttacgaacct tcaccggata atcggcaata ctttgcagat cgcgatagtg cttaacgatg
     2101 gtggtacgct cgcggatcag ctccagttct ttctctcgct gcggcgcaag ctccagttgt
     2161 ggatcgtgga tcaaaatggc gttttccaga tccagacgcc aggcgcgcgg gttcaggtta
     2221 ttaccggtga tcaacatcca cttatcatca acccacatcc ctttcaggtg ataggtgttg
     2281 tcgtcatctt tccataaccg aaccactagc tggtcagtat tgacgtaata ctgcaaacgg
     2341 ctcaggaaac gacgcagatt gatctcatag agataaggca atgcgccaat tatcttgaaa
     2401 ggttcatctt ccggaatgta gaagtcgttc gcggttttat caccaacaat aatttcgacc
     2461 tttttccctt cgcgcagcaa ctggataata ttgcgcacaa ggattgctgg caggttgaag
     2521 tatggcgtac agatggttag tttctgctcg gcacaaggca taagatggaa aatggtcttg
     2581 ttcaacagac tcgatttccc cagccccact agcggcgtta cggaaagctg atcgttgtcg
     2641 gcatcgccct ggaaatgata agcggcatca cgcagctcct ggcggaacag acgaatatcg
     2701 ttcttgattt ccgggctttt tggccgatta acatcatcca gacgattaac gccgcggcca
     2761 ttcataatat tctgtgtaac ccattcaaac ataatgtctg acatcttacg gttacggatc
     2821 agatgataac ggtcgtagcg atatttatcg tgttgatgca ggtaaacatc gttcaggctg
     2881 gcaccgctat aaagtacgct atcgtcgatg ataaagcctt taaagtgcag aacaccaaga
     2941 gcttcacggg tattgattgg aacgccataa accggaacat ctacgcccgg attttcctgc
     3001 gccatgcggc agtaccagtc agcgttagtg ttagatgccg cagcgccaat gcgtccacgt
     3061 tgtgcacgat gccagtcgac cagcacccgc acatccagtt ccggacgctg ccttttagcc
     3121 tcatacaacg cgttcagaat gcctttgcca ccgtcatcct gttcgagata cagggcgaca
     3181 atgcaaatgc gctgcttcgc gctggctatt ttttccagca gcgtctcccg gaagtcggcg
     3241 ggagcgtaaa agaaatcgac atcatcaact gattgagaaa tcttgggtag ttgggcaagg
     3301 tgttgttgat gtttattacg cttaaatttt gacaacatca cagtgcattt cttctctgtt
     3361 cattgaaggg tcctctgtgc aatgcagacg acataagcgg gcaataataa caccagtccc
     3421 gattaagtgg tcaacatttc cagtacctta ctcatgattc ctcgcgccgg gcaagattta
     3481 gcgtaagccc aacgatcccc tcttcgagct ggatatcaac gttaaacccg agcttgcggg
     3541 ctagcgccac catgccacga ttgtttggca tcgtaatacc attcagacgt tgtagtccgt
     3601 gatttcgcgt ataggtaatc aacttttcca ttaagcgtcg acctaagcct aaccctttga
     3661 gatccgagcg aaccagtaca gcaaattcgg catcgatgtt atcaggatcg gaaatcgcac
     3721 gcgtgacgcc gaggatctct tccgtttgat caatacgtcg taccgctaca aacgccattt
     3781 cccgatcgta gtcgatctgt gtcatgttgg ctaaatcttc atgggtaaat tcgttgatct
     3841 cgctaaagta gcggtaataa agatcttctt tggtgactcg cgaaatgaat tgctgaagtt
     3901 gtggctcatc ttctggcaaa atcgggcgga acaagcagcg ttcaccgttt ttcaattcta
     3961 cccattcttc cagctgatgc ggataagggc gcactgccag ccgactctcg ttatcgcctt
     4021 caaacggcga gatatccagc gtgacatcca gcgcggtaaa ttcactgcca gaagccagca
     4081 aaggatgaat atccagacgc tgaatttccg ggcaatcgac aatcaagttg gaaacctgca
     4141 ccagaagctg gctcaagcct gcaacatcca atgggcgtag cgcactgcgc gcacgaatct
     4201 ttttactttt gatcccctga ataaccagat agcgggccag gttcatgttc agcggcggca
     4261 gtgcgacgac ggcttgatct tcaggacgcc actccacacc gccttcaccc agcatgatca
     4321 acggcccgaa aaccggatcg tgctcaacca caacccgcaa ctcctgagcg ccagcacggt
     4381 tagccatact ttgcaccaac aggccgtgga cccgcgcctg tggccaggcc atttttacgc
     4441 gatcgaaaat agcgttcgcc gcttgctgga cttcattggc tgtacgcagg taaagcatga
     4501 cgccctgaac ttccgattta tgtggaatat ccggcgaacg caatttcagc gccaccggat
     4561 aaccaatctg ttcggcaata tgcaccgctt cggtgctatc gctggcaatc caggttggga
     4621 gcgtgttcat gccatacgct tgcaggatgg gctgaacttc atgggtatcg agcgacgtag
     4681 ccccttcggc aatcgcctgt tgcaacagaa gatgcgcttc tgcggtattg gaagtcagat
     4741 tgctgggcaa cgccggcgtt tcgcgtagtt gcttctgatt acgccggtac tccaccatat
     4801 gcataaaagc agtgatggtt ccttccgggg tacggtaggt cggcagcccg gcttcgctga
     4861 ataaacgtcg tgcctcttgc gaggagtgct cgccgcacca gttcgtcagc aaagagacat
     4921 atttgctgcg gggatgatgc tttaccgctt caattaatac ttgcgcgctt tctgttgcgg
     4981 gagcagcggc gctgggcgaa tgaataacca tcagtgcatc aaaatcctgg ctgtggagca
     5041 gaatgtccag cgttttgaca taatgctcgc tgctggcgtc atcgcgcaga tcgagcgggt
     5101 tagagactgc cacatgttct ggcaatgcat cgcgcaattt ctggcaggtt tcttcgctta
     5161 gcgttgccag cttgccattg cgtgaccata aggcatccag cgccagcgcg gcaggcgcag
     5221 caccgttgct gataatcatc aaccggtcgc cacgcagcgg gcgcatatgg ctaagggttt
     5281 ccaccgccga aaacagttcg tgggtatcct gtacccgcaa caaaccggca cgctgaatag
     5341 ccgcatccca tgccgggtcc attcctgccg tggtgttgag cagtcgctgt gccgccgggc
     5401 tacgtccgct tttaatcacc agaatcggtt tattacgcga ggcactacgg gccgccgaaa
     5461 caaagcgtcg cgcgtcgctt aactgttcga gatagagcag gatggcgctg gttttactgt
     5521 cgcgcgccag atagtcgagc aattcatcaa cgtcgatatc taggctgtcg ccgagcgcaa
     5581 taaagtagga aaagcccatc tcacgctgtt gcgcccagtc gaggatggtg ttggagacgg
     5641 cagccgattg cgaaataaac gccagcttgc cgcgtttaat cggcacaggc gaaaagctgg
     5701 cattcagacc ttgccaggga gccagtaaac ccagactgtt tggtccaagc aggcgcatgt
     5761 tatggcgcag ggcgcaggcg cggagatctt cgtgttgcga tgccggggcg gaaagaataa
     5821 tgcaggtttt acagcctttc tcgccgagct cttccagaag agcaagatta cggctggcat
     5881 tggtacataa aaccgcaagg tcgggtgtaa agggcaagct ggcaatatcc ggccaggcca
     5941 acacacccaa caccgctttc caggctggcg tcaccgggag taccggtccg ttaaagcctc
     6001 ccgccagcag gttacgcatc atcaggtaac ctgcgcgatt gggtttcatc gacgcgccaa
     6061 ttaccgctat cgattttggt cgcagtagtg cttccagtcc tcgctgactc ataccggtct
     6121 cccgcagaag tgaccgaatg attttaaacg ctttctaact gttctgctgt gatgctaccc
     6181 agatgttgcg tttttcctgc cagatagcgt gttttaaagc gggtaaaatg ctcgcctaac
     6241 cctgctgccg ccccggtatc gccggccata tctaacagtg cgatggctac ctcggcagta
     6301 caatattggc ctgcagcctg ggcttcacgc aggcgatagg cagaaagccg ggaaagatcg
     6361 acggaaatga cgggaagatt atccagatac ggacttttac gaaacatctt gcgagcttcc
     6421 ggccaggtac catcgagcat gataaacagc ggtggcttac cggcaggtgg tgtgaagatc
     6481 acttcccgtt gctcatcagc atacgaggcg ggaaagacca ccattggctg ataatccggg
     6541 ttttgtacca gatccagcaa atcctgcgag ggttcggtac gcgaccattg aaacgcaacg
     6601 gtatcaggca aaatatcagc aatcagacgc ccggtattac tgggcttcat tggctcggtg
     6661 tcgaacatca gcaaacagaa gcgacttttt gcttgtgctg gggtaattgt cgaacagaga
     6721 cataatttct ctggcaaaag acagcgttgg cagcgacgaa cgcgattacc gcgggcaaga
     6781 aaaggacgtg ttgcgcgcgc aatacgctcg gcgcgtaact ggagaacagc gttttcggtc
     6841 ataagagagc gtcgaaaaaa cgccattgtc gcagaggaga aaacggggca caagatgcgc
     6901 cccggtaaga ttaaagagat tcgttcagcc agctatcgaa cggcgctttc ggtactgcgc
     6961 cattaagcat gtcgacaacc tgaccgtttt tgaaaatcat gatcgtcggt atactacgaa
     7021 ttccaaagcg actgctcaat tcacgttcag cttcggtatt cactttcaca aagcgcactt
     7081 taccgctacg ctcttgcgcg acatcttcaa aaattggtgc gaaattacgg caggggccgc
     7141 accacggtgc ccagaagtcg atcaccacag gtagatcatc cttcagcaat ttgtcgagtg
     7201 tttcaccggt cgcattaatc acctctccgt caaacaagtc gtgaccgcag cgtccgcatt
     7261 ttgccgcatc ttcgatccga tcgtcgggaa tgcgattgat ggcctgacaa tgggtacaaa
     7321 cggtattcat aactaacctc gggatgagta aggagactta tcggcaacga cgccggtatg
     7381 tttctaatat gttacatatt atcggggaca ttgttccaga caacaatctg ttttattcaa
     7441 taggtatgat agtcgcaggc tttgctacga aatcatggcg aaccgcgcgg acttcgggta
     7501 atctgcgcgc ttcgcgcagc gctggtggag aaaagcatga acgatgaaat gaaaggtaaa
     7561 agcggcaagg tcaaagtgat gtatgtccgc agtgatgatg attctgataa acgtacccac
     7621 aacccgcgta ccgggaaagg gggcgggcgt ccaggaaaat ctcgtgctga tggtggccgt
     7681 cgccccgccc gcgatgacaa acagagtcag ccccgtgacc gcaagtggga agattcgccg
     7741 tggcgcacgg tttcccgcgc gccgggtgat gagacgccgg aaaaggccga tcacggtggc
     7801 atcagtggta aaagttttat tgatccggaa gtgttgcgtc gtcagcgtgc ggaagaaacc
     7861 cgcgtctacg gcgaaaacgc ctgtcaggca ctgttccaga gccgtccgga agcgattgtt
     7921 cgcgcctggt ttatccagag tgtaacgccg cgttttaaag aagccttgcg ctggatggca
     7981 gcaaaccgca aagcgtacca tgtggtggat gaagcggaat tgacaaaagc gtcaggtacg
     8041 gaacatcacg gcggcgtttg cttcctgatc aaaaagcgta acggtacaac cgtgcagcag
     8101 tgggtaatcc aggcaggcgc gcaggattgc gttctggcac tggaaaacga atctaacccg
     8161 cataacctgg gtggcatgat gcgcagctgc gcgcacttcg gcgtgaaagg tgtcgtggtg
     8221 ctggatgcgg cactgctgga gtcgggggcg gctatccgta ccgcagaagg cggcgcagag
     8281 cacgttcagc cgattactgg cgacaacatt gttaacgtgc tggatgattt ccgtcaggca
     8341 ggttacaccg tagtgaccac ttccagcgag cagggtaaac cgctgttcaa aaccagtctg
     8401 ccagcgaaaa tggtactggt gctgggtcag gaatatgaag ggttaccgga tgccgcacgc
     8461 gatccgaacg atctgcgcgt gaagattgac ggtacgggca acgttgccgg gctgaatatc
     8521 tctgtcgcaa ccggcgtttt gcttggtgaa tggtggcgtc agaataaagc ctgataaatc
     8581 agccgggtgg caactctgcc atccggcatt tccccgcaaa tttactcaca ctctgccggt
     8641 aatactggca tccagtcaat cggcgtctcg ccacgttgtt ccagccactg atttgccagc
     8701 acaaaatggt tgcagccaaa gaatccacga tgcgccgaaa gcggcgacgg atgcggtgct
     8761 ttcagtacat gatggcgttg cttatctata atcgcccctt tcttttgcgc atgtgatccc
     8821 cacaacaaaa acaccacgcc ttcgcggtgc tggttaatca ggctgatgac tttatcagta
     8881 aacgtctccc aaccgaggct ggcgtgggaa tgcgcctgac ctgcgcgaac cgtcaacaca
     8941 gtattgagca gcagaacgcc ctgacgcgcc cagctttcga gatagccatg attagggcgg
     9001 gtgaagcccg gaatagtatt ttccagctct ttatacatat tcaataatga cggaggaatg
     9061 gcaatgccgg gacgaacgga aaatgccaga ccatgcgcct gtcccggtcc gtgataagga
     9121 tcctggccga gaatcaccac tttaacgtca cccaactcgg taaagcggaa cgcgttaaag
     9181 acatcttttt gtggtggata gatagtgacg ccggactgcc gctcgctggc gacggtctga
     9241 agggtattaa gaaaataagg ttgttgcttc tcttcagcca gcacgtcatg ccaggttaat
     9301 tcgttagcca tcttgctctc ctgcgaatct tcaatccgcc tagcttaact gcttctttct
     9361 acagaacaaa atcatgctca ttttttagac ttaaacgagt gcaaaaaaat gtaaattggt
     9421 aaaatttcac acccgcataa gttgatgtaa aacaataaaa tccatttacg accacaatcg
     9481 atgtgtggtt tttgttgatt taaatcaaag aatcaagggt ggttgaggag tatatataca
     9541 ctcaagcaac aatggtttta ccaattggcc gcgacaggct gaacaaatca aataattttg
     9601 ccggggaggc atcacatgat tacaggtatc cagattacta aagccgctaa cgccgatctg
     9661 ctgaactctt tctggctgct ggacagcgaa aaaggcgaag cacgttgcat cgttgcaaaa
     9721 gcaggttatg cagaagatga agtggttgca gtaagcaaac tgggtgacat tgaataccgt
     9781 gaagttccag tagaagtgaa accagaagtt cgcgttgaag gtggtcaaca cctgaacgtt
     9841 aacgttctgc gtcgtgaaac tctggaagat gcagttaagc atccggaaaa atatccgcag
     9901 ctgaccatcc gtgtatccgg ttatgcagtt cgctttaact ctctgactcc ggaacagcag
     9961 cgcgacgtta tcgctcgtac ctttactgaa agcctgtaag gcttaagtag caagttacta
    10021 agcggaagag ggcgacctct tccgcttttt ttcgttaata gaaaatgcgt accgcgcaat
    10081 agaccagcaa cagggcaagc acgatattta actggcgacc atactggcga aacaatcgct
    10141 gaaacagatg ccccgccagc gcccagcaca cattgccaaa cgtcccaatc atcgccagca
    10201 aaacgctgac gccaactacc cagcttagcg cctgtgtttg cggcagaaca aacgtcgaca
    10261 gtgccgtaac accgtacaaa atgattttga cgttcacaaa ctgcaaagca aagctggccc
    10321 aaaagctgat tggttttgcc tgaagtccgt cttcctttgt tgggctggtg gcgattttcc
    10381 acgccagcca gacaatatat gccgcccccg cccaactcaa aaggtgtacc gctgccgggt
    10441 caatcactgc cagtgaaaat gaaatgcccg cacacagtaa catcacaatc aaaaatccca
    10501 gactcatccc tgccagcacg cgggtacttt gacgaaatcc atgcgacgta gcagagctaa
    10561 gggcgagaat attgttcggt cctggcgtca tagcggtaat cagggtgtaa gtccaaaaag
    10621 cacttaaaag gatcggtgtc acttgttatc tcctctgttt tggtagacag tacccgacac
    10681 ggaacattta aaaaaatgaa tgtttttata ttctaaattc gatattttcg ataggtttgg
    10741 ggttatggat ctgcgccgtt ttattacgct taaaaccgtg gtggaagagg gttccttttt
    10801 gcgagcttcg caaaaattgt gctgtacaca atcgacggtg acttttcata ttcagcagct
    10861 tgagcaggag ttttcagtcc agttatttga gaaaattggc cgacggatgt gccttacccg
    10921 cgaaggaaaa aagttgctgc cgcacattta tgaacttacc cgagtgatgg atacgcttcg
    10981 tgaggctgcc aaaaaggagt cagatccgga cggggaacta cgcgttgttt cgggcgaaac
    11041 gttgctctct taccgaatgc cgcaggtgtt gcagcgtttt cggcaacgcg caccaaaagt
    11101 gcgtttatcg ttgcaggcgc tgaactgtta cgtgatccgt gatgcgctac tgaatgatga
    11161 ggccgatgtc ggcgtctttt atcgcgtagg gaatgatgat gccctgaacc gacgagagtt
    11221 gggtgaacaa tcgctggtgc tggtggcttc accacaaatt gcagatgtcg attttactga
    11281 gccgggaaga cataacgcct gtagctttat tatcaacgaa ccgcaatgtg tcttccggca
    11341 gatatttgag agcacgctgc gacagcggcg gatcacggtg gaaaacacca ttgagcttat
    11401 aagtatcgaa agcatcaagc gttgtgtcgc ggcgaatatc ggtgtcagtt acttgcccca
    11461 ctttgcggtg gaaaaagaga ttgagagcgg agaattaatt gaattgcctt ttggcgaaca
    11521 gtcgcagacc attacggcaa tgtgcgcaca ttatgctgga aaagcggtta gcccggcgat
    11581 gcacattttt attcagtgtg ttgaagagtg ttttgtggtt gcgtaaaaaa acaatgccgg
    11641 gcttaaaccc ggcattgaga ttactcttct gtcgtttgcg gtggcacgcc cgttccgctc
    11701 ggtttacggc gcttaccaat atttttggtg tcgcgatggc gttttttcac ccgcggcttc
    11761 tctttctctt tagctttttt cttctcggcg cgttttgcca gtactttctt cgatggcttg
    11821 ccggtctgct tctcgcttgg cgcacgcgtt ttcgggcgta actcatcaat aacgcgagct
    11881 ttaatcggct cttcaatgta acggccaacc ttgcccagca gcaaatggtc atgggcttcc
    11941 accagcgaaa ttgcggtgcc tttacgaccg gcgcgcgcgg tacgtccgat acgatgcaaa
    12001 taagtatcgc cactgcgcgg catatcgaag ttaaagacgt ggctgacgtc aggaatgtcg
    12061 ataccgcgcg cggcaacatc ggtggcaacc agtacgttta cgcggccttc ggtcaggcgt
    12121 ttaattgctt cgttacgctt gccttgtacc atctcacctt cgagatagca gttgttgatg
    12181 cccgcttcgc gcagccagtt tgccagctca tgcacacgtt cacgcttacg cacaaacacg
    12241 attgagcgag tcgcttcagg ctgttttaac aggtgcacca gcaatgcggt tttatgttcc
    12301 aggtcatcgg cgcggtaata ccactgatga atttttttgc gctcacgggt ggacggattg
    12361 gcagaaactt ccaccggatc ttccagcaga cgctcggcaa agtcctgaat cgcatcgcct
    12421 tccagcgttg ccgaaaagag cagggtctgt ttacgccagc gagtttcgcc agcaatatgt
    12481 tcgatatcct gagcgaagcc catatccagc atacggtctg cttcgtcgag gatcagcgtt
    12541 tcaaccgcgc ggcaatcgaa gttctcttct tttatgtatt gcagcagacg tccggtcgtg
    12601 gcgaccacga tgtcctgatt ttcgctgaac acttccgcgt ggttcatata ggctacgccg
    12661 ccggtgatgg tggcgatatc cagatgcgta tgtttcgcca gttcgcgggc atgatcggcc
    12721 acctgcatcg ccagctcgcg agttggggtg aggatcaaaa tacgcggcgg accggatttc
    12781 ttacgtggga aatcgagcag gtgctgcaac gctggcagca gatacgccgc cgttttaccg
    12841 gtgcctgtcg gcgcagaacc gagtacatca cggccatcga gcgcaggcgg aatggcggca
    12901 gcctgaatgg cggtcgggcg agtgaaacct ttatcctgga gggcttccag caggctttcg
    12961 tcgagttcaa gttcggaaaa agtcgttaca gtcatgttct acctctgtgt ggggcgctga
    13021 ttatagacgt tacggctgca atcttcatct gtttgtatgg atatcgcttc tcgggtattg
    13081 ttctcacccg gtgctatcgc cgtttttcct gcaaggtttt attttcatgt cacagtctac
    13141 atccgtgctt cgtcgtaatg gatttacttt taaacagttt tttgttgctc acgatcgctg
    13201 tgcgatgaaa gtgggaacgg atggcatttt gctgggcgca tgggcaccgg tggctggggt
    13261 gaaacgttgc cttgatatcg gcgcgggtag cgggttgctg gcactgatgc tggcgcagcg
    13321 aaccagtgac agcgtgataa ttgatgccgt tgagctggaa agtgaagctg ccgcgcaggc
    13381 gcaggaaaat atcaaccagt ccccgtgggc agagcggatt aacgtccata cggcggatat
    13441 tcagcagtgg atcacacagc agacagtacg cttcgattta atcatcagta acccacctta
    13501 ctatcagcag ggagtagagt gcgcgacacc tcaacgggaa caggctcgct ataccactac
    13561 gcttgatcac ccatcgctgc tgacctgtgc ggcggagtgc attaccgaag agggattttt
    13621 ctgcgtggtg ctgccagagc agattggtaa tggttttact gaactggcat taagcatggg
    13681 ctggcattta cgtttgcgga cggatgtggc ggaaaacgaa gcgcgactgc cgcatcgggt
    13741 gctgctggca ttctccccgc aggcgggaga atgctttagc gatcgcttag tcattcgtgg
    13801 gccagaccag aactattccg aagcgtatac ggcgctgacc caggcttttt atctgtttat
    13861 gtaatgattg ccgggggaaa ggatcgatgg accggaatgc gtgagcagtt ccgggtaatc
    13921 cagcgtaaaa tgcagccccc ggctttcttt gcgcatcatc gcgcagcgaa caatcaactc
    13981 ggcaacctgt accagattac gcagctccag caaattattt gagacgcgga aatgggcgta
    14041 atattcgtct atttcttgtt ggagcatggt tatccgccgc agggcgcgtt ccaggcgctt
    14101 cgttgtgcgc acaatgccaa cgtaatccca cataaacagc cgtagctcgt gccagttatg
    14161 ctgaattact acccgttcgt cagggttctc aacgcggctt tcatcccacg gcggtaacgt
    14221 actgacgccg tgggcataag gcatacgtct ggtgatatct tctgccgccg accagccata
    14281 gaccagacac tccagcaatg aattcgaggc catgcggtta gcgccgtgta agccggtata
    14341 actcacctcg ccaatggcat acaagccctc gacgtccgta cgcccatgat catcaaccat
    14401 tacaccacct caggtatagt gtgcagcagg cacaatcggt accggttctt gtgtgagatc
    14461 aatccccagc ccgagcaatt tttcataaat catcgggaaa tgctggcgaa taaaatcggc
    14521 gggcttatgg ctgatgtcga ggaacataca atctgcgccg aggcgtttca tttcatggtc
    14581 aatggcgcgg gcgacaatat cgcgcggggc cagttcgccg cgctcatcaa aatcgggcat
    14641 aaaacgcgtg ccatccgggc gcttgagata agcgccttcg ccgcgcagtg cttccgttaa
    14701 caggaaattg cgtgcctgtg ggtgatacag tgcggtaggg tggaactgat taaattcgag
    14761 attggcaacc cgacagcccg cgcgccacgc catagcaatg ccatcgccag aagaaatatc
    14821 cggattggtg gtgtactgat acaccttcga cgcaccgccg gttgccagta ccactgcttt
    14881 tgcgtggcag gtttccaccg tttctttatt tcggttccat acccacgcgc ctacaacccg
    14941 tcgcgtgccc ggcaggccaa ttttgtcaga gacaatcaga tcaactgcgt tgctgcgctc
    15001 cagcacacga atattcggat ggttctgcgc cttgctcacc agcgtggatt gtacttctct
    15061 accggtggcg tcggcggcat gaagaatacg acggtgacta tgtccacctt cacgggtcag
    15121 atggtagctt tcttcgccat tcggttgaac gtgggtatca aacaaaaccc cctggtcaat
    15181 taaccatggc acacaggaac gagcattgct ggcgacaaat tcaactgcat ggtgatcgca
    15241 aataccagcc ccggcaatca atgtgtcttc cacatgcgag tcaatgctgt cagtttcatc
    15301 aaacacggcg gcaataccgc cctgggcata aaatgttgaa ccttccgtta ccgggccttt
    15361 acttagaacg atgacctgat gctggtcagc caggcgtagc gccagtgaaa gtccggctgc
    15421 gccgctacca ataatcaaca cgtcacatga atgttcaggg agagtattca ttttctttgt
    15481 ttaatttact aaacattgtt tggtcggcat agcatcatgt tgtgcgaata aacacctgct
    15541 attttaatat ttgttacagt ggctaaacac gttgacgcag ggcggcgaga aaaagagaag
    15601 ttactggctg gtggaggatt aggtggtgaa ataaaaaggc cgttgggtta ctcttcaggc
    15661 agttaaatgg gcatttctac acagataatg cgatgttcag attctgtaga cttataatga
    15721 tagataatga tccgtctaca gcatgacaaa caaaaacaga tgcgttacgg aactttacaa
    15781 aaacgagaca ctctaaccct ttgcttgctc aaattgcagc taatggagtg gcgtttcgat
    15841 agcgcgtgga aatttggttt ggggagactt tacctcggat gagcgagcag ttaacggacc
    15901 aggtcctggt tgaacgggtc cagaagggag atcagaaagc ctttaactta ctggtagtgc
    15961 gctatcagca taaagtggcg agtctggttt cccgctatgt gccgtcgggt gatgttcccg
    16021 atgtggtaca agaagctttt attaaagcct atcgtgcgct ggattcgttc cggggagata
    16081 gcgcttttta tacatggctg tatcggattg ctgtaaatac agcgaaaaat tacctggttg
    16141 ctcaggggcg tcgtccacct tccagtgatg tggatgccat tgaagctgaa aacttcgaaa
    16201 gtggcggcgc gttgaaagaa atttcgaacc ctgagaactt aatgttgtca gaagaactga
    16261 gacagatagt tttccgaact attgagtccc tcccggaaga tttgcgcatg gcaataacct
    16321 tgcgggagct ggatggcctg agctatgaag agatagccgc tatcatggat tgtccggtag
    16381 gtacggtgcg ttcacgtatc ttccgagcga gggaagctat tgataacaaa gttcaaccgc
    16441 ttatcaggcg ttgacgatag cgggatactg gataagggta ttaggcatgc agaaagaaca
    16501 actttccgct ttaatggatg gcgaaacgct ggatagtgag ctgcttaacg aactggctca
    16561 taacccagaa atgcagaaaa cctgggaaag ctatcactta atccgtgatt caatgcgggg
    16621 tgatactccc gaggtgctcc atttcgatat ctcttcacgc gtgatggccg ccattgaaga
    16681 agagccagta cgtcaatcgg cgacattgat cccggaagcc cagcctgcgc cgcatcaatg
    16741 gcagaaaatg ccattctggc agaaagtacg tccgtgggcg gcacagctta cccaaatggg
    16801 cgtagccgca tgcgtatcgc ttgcagttat cgttggcgtc cagcactata atggacaatc
    16861 tgaaacgtcc cagcagcccg aaacgccggt atttaataca ctgccgatga tgggtaaagc
    16921 cagcccggta agcctgggag taccttctga agcgaccgca aacaatggtc aacagcagca
    16981 ggtacaggag cagcgtcgtc gcattaatgc aatgttgcag gattacgaac tgcaacgccg
    17041 actccactct gaacagcttc agtttgagca ggcgcaaacc cagcaagccg ctgtacaggt
    17101 gccaggaatt caaactttag gaacgcaatc gcagtaatga agcaactttg gtttgccatg
    17161 tcattagtga caggtagcct gttattctct gctaacgcct cggccactcc cgcgtccggg
    17221 gcgttattac agcagatgaa cctggccagt cagtcactga attacgagct gtcattcatc
    17281 agcatcaata aacagggtgt tgagtctctg cgttatcgac atgcacgcct cgataaccgt
    17341 cctcttgcac aattgttgca aatggatggc ccgcgccggg aagtggtaca gcgcggcaat
    17401 gaaatcagct attttgaacc gggacttgaa ccgttcacgc ttaatggcga ttacattgtt
    17461 gattctctgc catcgcttat ctataccgat ttcaaacgcc tttctcctta ctacgacttt
    17521 atctccgtcg ggcgcacgcg tattgctgat cgtctttgcg aagtcattcg cgtggttgcc
    17581 cgagatggta cacgctacag ctacatcgtg tggatggaca ccgaatcgaa attaccgatg
    17641 cgggttgatc tccttgatcg cgatggtgaa acgctggaac aatttcgcgt gattgctttt
    17701 aacgtcaatc aggatatcag cagcagtatg cagacgctgg cgaaggcaaa tttgccgccg
    17761 ttgctttctg ttcctgtagg tgaaaaagct aaattcagct ggacgccaac ctggttgcca
    17821 cagggtttta gcgaagtttc cagtagtcga cgtccgctac cgacgatgga caacatgcct
    17881 atcgaatcac gtctctattc cgacggatta ttcagcttct cggtaaacgt taaccgcgct
    17941 acgccatcga gcaccgatca gatgttgcgc accggacgca gaaccgtcag tacaagcgta
    18001 cgtgataacg ccgaaatcac cattgtcggt gaactgccgc cgcaaacggc gaaacgcatt
    18061 gccgagaata ttaagttcgg ggcagcgcaa tgatcaaaga gtgggctacc gtcgtctcct
    18121 ggcaaaacgg gcaggcgctg gtcagttgtg atgttaaagc ttcatgcagc agctgcgctt
    18181 cacgcgccgg ttgcggtagc cgcgtgttga ataaacttgg cccgcaaacc acgcatacca
    18241 ttgtcgtacc ctgtgatgag ccgttagtgc cggggcaaaa agtggagttg gggatcgccg
    18301 aaggcagcct gcttagctcc gcattactgg tttatatgtc gccgctggtg ggattattcc
    18361 tcatcgcttc gctatttcag ctactctttg cttctgacgt cgcagcatta tgtggtgcgg
    18421 tcctcggcgg tattggcggg ttcctgattg cgcgcggcta ctcgcgtaag tttgctgccc
    18481 gggcagaatg gcaaccgatc atcttaagcg tggcattgcc gcctggcctt gtgcgatttg
    18541 aaacatcttc ggaagacgcg agccagtgat ttcactggct ttgttgcatt ttgcataaca
    18601 aatgcgagga cgtttccaga taccacaatc catacggtat gaacatttcc tcgcctcaat
    18661 gttgtagtgt agaatgcggc gtttctatta atacagacgt taagctcaga acagcgactt
    18721 ctaaaagcct ggttaaccgg gcattaaggc acaataatca tactttatga agaatatacg
    18781 taacttttcg atcatagctc acattgacca cggtaaatcg acgctgtctg accgtattat
    18841 ccagatctgc ggtggcctgt ctgaccgtga aatggaggcg caggttctcg attccatgga
    18901 tcttgagcgt gagcgtggca ttaccatcaa agcgcaaagc gtgacgctgg actacaaagc
    18961 gtctgacggc gaaacctatc agcttaactt tatcgacacc ccgggccacg tagacttctc
    19021 ctatgaagtt tcccgttcgc tggctgcctg tgaaggtgca ttgctggtgg tcgacgccgg
    19081 gcagggcgta gaagcgcaaa ccctggcaaa ctgctacacc gccatggaaa tggatctcga
    19141 agtggtgccg gtactgaaca agattgacct gccggcagcc gatcctgaac gcgtggcgga
    19201 agaaattgaa gatatcgtcg gcatcgacgc caccgatgca gtgcgctgtt cagcgaaaac
    19261 cggcgttggc gtgcaggacg ttctcgaacg tctggtgcgc gacattccgc cgccggaagg
    19321 cgatccggaa ggcccgttgc aggcactaat tatcgactcc tggttcgaca actacctggg
    19381 cgttgtttca cttatccgta ttaaaaacgg caccctgcgt aagggcgaca aagtgaaagt
    19441 catgagtacc gggcagacct ataacgccga ccgtctgggc atcttcacgc cgaaacaggt
    19501 tgaccgcact gaactgaaat gtggcgaagt aggctggctc gtatgtgcga ttaaagatat
    19561 ccacggcgct ccagtcggcg ataccttaac gctggcgcgt aatccggcag aaaaggcgct
    19621 gcctggcttt aagaaagtca aaccgcaggt atacgccggt ctgttcccgg taagttccga
    19681 cgactatgaa gccttccgtg acgcgctggg taaactcagc ctgaacgatg cctcactgtt
    19741 ctatgagccg gaaagctcca gcgcgctggg ctttggtttc cgctgcggct tccttggcct
    19801 gctgcacatg gagatcatcc aggagcgtct ggaacgtgaa tacgatctgg atctgatcac
    19861 cactgcgccg accgtagtgt atgaagttga aaccacgtca agggaagtta tctacgtcga
    19921 cagcccatcc aagctgcctg cggtaaataa catttacgaa ctgcgcgagc cgattgcaga
    19981 gtgtcacatg ctgctgccgc aggcatatct cggcaacgtt attacgctgt gcgtagaaaa
    20041 acgcggcgtg cagaccaata tggtttacca cggtaatcag gtggcgctga cgtacgagat
    20101 cccgatggcg gaagtggtgc tcgacttctt cgatcgcctg aaatctacct cgcgtggtta
    20161 tgcgtctctg gattacaact tcaaacgctt ccaggcgtcc gacatggtac gtgtagacgt
    20221 attaatcaac ggtgaacgtg ttgatgcgct ggcgttgatc acccaccgtg ataattcgca
    20281 aaaccgcggt cgcgagttgg tggagaagat gaaagatctg atcccacgcc agcagtttga
    20341 tatcgccatt caggcagcga ttggtacgca cattattgcg cgatccaccg tgaaacagct
    20401 gcgtaaaaac gtactggcta aatgttatgg cggcgatatc agccgtaaga aaaagctgct
    20461 gcagaagcag aaagaaggta agaaacgcat gaagcagatc ggtaacgttg agctgccgca
    20521 ggaagcgttc ctcgccattc tgcacgtcgg caaagacaac aaataaccct taggagttgg
    20581 catggcgaat atgtttgccc tgattctggt gattgccaca ctggtgacgg gcattttatg
    20641 gtgcgtggat aaattctttt tcgcacctaa acggcgggaa cgtcaggcag cggcgcaggc
    20701 ggctgccggt gactcactgg ataaagcaac gttgaaaaag gttgcaccga agcctggctg
    20761 gctggaaacc ggagcttctg tttttccggt gctggctatc gtattgattg tacgttcgtt
    20821 tatttatgaa ccgttccaga tcccgtcagg ttcgatgatg ccgactctgt taatcggtga
    20881 ttttattctg gtagagaagt ttgcttatgg cattaaagat cctatctacc agaaaacgct
    20941 gatcgaaacc ggtcatccga aacgcggcga tatcgtggtc tttaaatatc cggaagatcc
    21001 aaagcttgat tacatcaagc gcgcggtggg tttaccgggc gataaagtca cttacgatcc
    21061 ggtctcaaaa gagctgacga ttcaaccggg atgcagttcc ggccaggcgt gtgaaaacgc
    21121 gctgccggtc acctactcaa acgtggaacc gagcgatttc gttcagacct tctcacgccg
    21181 taatggtggg gaagcgacca gcggattctt tgaagtgccg aaaaacgaaa ccaaagaaaa
    21241 tggaattcgt ctttccgagc gtaaagagac actgggtgat gtgacgcacc gaattctgac
    21301 agtgccgatt gcgcaggacc aggtggggat gtattaccag cagccagggc aacaactggc
    21361 aacctggatt gttccgccgg gacaatactt catgatgggc gacaaccgcg acaacagcgc
    21421 ggacagccgt tactggggct ttgtgcctga agcgaatctg gtcggtcggg ccacggctat
    21481 ctggatgagc ttcgataagc aagaaggcga atggccgact ggtgtgcgct taagtcgcat
    21541 tggcggcatc cattaatagc catcttcgtt cacgttgtcg ccgttatggc gacaacgtga
    21601 attatttatg agataaatct cccgtggcta acgacatccc ccgtcgttgt gtatagaata
    21661 ttcccccgaa gtttaaggtt ggcaccttca ggttgccacg gcacacgaaa cagcgttggt
    21721 ccccatatac cgctaaactg aaactgcagc gaagcagtta gcagagccat gtatatcagg
    21781 tctgtttcgt gtgctgaatt gttgacgcat ttatttattg gtatcgcatg aaccccatcg
    21841 taattaatcg gcttcaacgg aagctgggct acacttttaa tcatcaggaa ctgttgcagc
    21901 aggcattaac tcatcgtagt gccagcagca aacataacga gcgtttagaa tttttaggcg
    21961 actctattct gagctacgtt atcgccaatg cgctttatca ccgtttccct cgtgtggatg
    22021 aaggcgatat gagccggatg cgcgccacgc tggtccgtgg caatacgctg gcggaactgg
    22081 cgcgcgaatt tgagttaggc gagtgcttac gtttagggcc aggtgaactt aaaagcggtg
    22141 gatttcgtcg tgagtcaatt ctcgccgaca ccgtcgaagc attaattggt ggcgtattcc
    22201 tcgacagtga tattcaaacc gtcgagaaat taatccttaa ctggtatcaa actcgtctgg
    22261 acgaaattag cccaggcgat aaacaaaaag atccgaaaac gcgcttgcaa gaatatttgc
    22321 agggtcgcca tctgccgctg ccgacttatc tggtagtcca ggtacgtggc gaagcgcacg
    22381 atcaggaatt tactatccac tgccaggtca gcggcctgag tgaaccggtg gttggcacag
    22441 gttcaagccg tcgtaaggct gagcaggctg ccgccgaaca ggcgttgaaa aaactggagc
    22501 tggaatgagc atcgataaaa gttactgcgg atttattgcc atcgtcggac gtccgaacgt
    22561 tggcaaatcc acattgttga acaaactgct ggggcagaaa atctccatca cctcccgcaa
    22621 ggcgcagaca actcgtcacc gcattgtggg gatccatact gaaggcgcgt atcaggcgat
    22681 ctacgtcgat acaccgggcc tgcatatgga agaaaaacgc gccattaacc gcctgatgaa
    22741 caaagcggcg agcagctcta ttggcgatgt tgagctggtg atttttgtcg ttgaaggcac
    22801 ccgctggacg ccggacgacg aaatggtgct caacaaactg cgcgaaggca aagcgccggt
    22861 aatcctcgcg gtgaacaaag tggacaacgt gcaggataaa gccgatctgc tgccgcacct
    22921 gcagttcctg gcaagccaga tgaacttcct cgatatcgtg ccaatctctg ccgaaaccgg
    22981 gctgaatgtt gacactattg cggcaatcgt gcgtaagcat ctgcctgaag cgactcatca
    23041 cttcccggaa gattacatca ccgatcgctc acagcgtttt atggcgtctg aaatcatccg
    23101 cgaaaaactg atgcgtttcc ttggcgctga actgccgtac tccgtgaccg tggagatcga
    23161 acgtttcgtc tctaacgaac gcggcggttg tgacatcaac ggtttgattc tcgttgagcg
    23221 tgaagggcag aagaagatgg ttattggcaa caaaggggcc aagatcaaaa ccatcgggat
    23281 tgaagcgcgt aaagacatgc aggaaatgtt cgaagcgcct gtccaccttg aactgtgggt
    23341 aaaagtgaaa tcgggttggg ccgacgacga acgcgcactg cgcagtctcg gttatgttga
    23401 cgatctttaa gagccactcc gatggaaggc tggcagcgcg catttgtcct gcatagtcgc
    23461 ccgtggagcg aaaccagcct gatgctggac gtcttcacag aggaatcggg tcgcgtgcgt
    23521 ctggttgcca aaggcgcacg ctctaaacgc tctactctga aaggtgcatt acagcctttc
    23581 acccctctct tgctacgttt tggcgggcgt ggcgaagtca aaacgctgcg cagtgctgaa
    23641 gctgtctcac tggcgctgcc attaagcggt atcacgcttt acagcggtct gtacatcaac
    23701 gaacttctct cccgcgtact ggaatacgag acgcgcttct ctgaactctt tttcgattac
    23761 ttgcactgca ttcagtctct tgcaggtgac actggtacgc cagaacccgc gctgcgtcgc
    23821 tttgaactgg cactactcgg gcatctgggc tatggcgtca attttaccca ttgcgcgggt
    23881 agcggcgagc cggtagatgg caccatgacg tatcgttatc gcgaagaaaa agggtttatc
    23941 gcaagcgtcg ttatcgacaa taaaacgttc accggaaggc agttaaaagc gttaaacgcg
    24001 cgggaatttc ctgacgcaga cacactacgc gccgcgaaac gctttacccg aatggcgctt
    24061 aagccgtatc ttggcggtaa accattaaag agcagggaac tgttccggca gtttatgcct
    24121 aagcgaacgg tgaaaacaca ttatgaatga tgaggattgt catggctgaa ttactgttag
    24181 gcgtcaacat tgaccatatt gcaacgctgc gtaacgcacg cggtactgct tacccggatc
    24241 cggtgcaggc ggcgtttatt gccgagcagg cgggagcgga cggcattacc gtgcatttac
    24301 gtgaagatcg ccgccacatt actgaccgcg atgtgcgcat cctgcggcag acgctggata
    24361 cccgaatgaa tctggagatg gcagtgaccg aagagatgct ggcgatcgcc gttgagacga
    24421 agccacattt ttgctgcttg gtaccggaaa agcgtcagga agtaacaacc gaaggcggcc
    24481 tggacgttgc agggcagcgt gaaaaaatac gcgatgcctg caaacgtctg gcagatgccg
    24541 ggattcaggt ttctctgttt atcgacgccg atgaagagca gatcaaagct gcggcagagg
    24601 ttggcgcacc gtttatcgag atccacaccg gttgctatgc tgatgccaaa actgacgccg
    24661 aacaggcgca agagctggtg cgtatcgcca aagccgcgac ctttgccgct agtctcggtc
    24721 tgaaagttaa cgccggacac ggtctgacct atcacaacgt gaaagccatt gccgccatcc
    24781 ctgagatgca tgaactgaat atcggtcatg ccattattgg ccgtgcagtg atgaccggac
    24841 tgaaagatgc ggtggcagaa atgaagcgtc tgatgctgga agcgcgtggc taatggcaat
    24901 attaggttta ggcacggata tcgtagagat cgctcgcata gaagcagtga tcgcccgatc
    24961 cggtgagcgc ctggcacgcc gcgtattaag cgataacgaa tgggcaatct ggaaaacgca
    25021 ccaccagccg gtgcgttttc tggcgaagcg ttttgctgtg aaagaagccg cagcaaaagc
    25081 gtttggcacc gggatccgca atggtctggc gtttaatcaa tttgaagtat tcaatgatga
    25141 gctcggcaaa ccacggctac ggctatgggg tgaggcatta aaactggcgg aaaagctcgg
    25201 cgttgtaaat atgcatgtaa cgctggcaga tgaacgccac tatgcttgtg ccacggtaat
    25261 tattgaaagt taaaataata ccgttctctt taaaaagtaa aacccccgaa ctggtaagga
    25321 acgggggttt tactttttaa ctttaatttt gtgcagcatg caccctcaac aacagcgatt
    25381 atggatcagt gtacttatct tttacaagca acttgcaatc tttagcataa aaactcgagc
    25441 ctttacgaag aaagcaatat tgatggaaag attaacgtga ccgccaattc gtaagtacat
    25501 taaaattggc ttcgttattg aagattttgc tgtgctttac accatgccac agaattcccc
    25561 cattgaaacg agtggtgtcg tcaaagctct ggtgtggagt gcagcatgca ccctcaataa
    25621 ctcgcacgtt cagttttggg gagatgtaag ggctaatctg aatggctgca ttccttgttt
    25681 aaggaaaaac gaatgactga ttgccgatac ctgattaaac gggtcatcaa aatcatcatt
    25741 gctgttttac agctgatcct tctgttctta taacacaagg aaacgtactt aaggtgcgtc
    25801 cggtgaacca atcggacgcg cctttaataa ctataaataa gtgtcttggc agatactata
    25861 taaattaact tagtgaatga ttatgctaat gtcatcaatt aaataaatat aatggcgtta
    25921 aggcttccca gtaatataat taatactcta cttccagagt agaatattaa attttatccg
    25981 cgtggtgcat cagcacaaat ttatcccaca actgttcttc tgtctcgaca tgcgccggat
    26041 ctttcacaat agtattgggg atcgggcaca ccttctggca ggttggtgtc tcgtagtgcc
    26101 ctacgcattc ggtacactta tcgctgttaa tttcgtagat atgatctccc attgaaatcg
    26161 cctcattcgg gcattcgggt tcacacatat cacaattaat acagcgttta gtaattagta
    26221 aagacatttc aatggattac cgttaaatca ttttaaaatc agtaagttgt atcgagtttg
    26281 tatgctttac tgtcattaac ttactgtatg ttgatccagt gtatttaacc ttgataaact
    26341 cagtccagca acacaaaacc gcaacacatt gcattttgtc ccgtagaaaa gacttgtatg
    26401 tgtgagcttg ttttctgcgc ctacgcagat aaggattgag aatgccgcgc actgtaacac
    26461 ataatccgga tagccccaat aatgacgatg ttttagccgc atctgaaaaa tgggacgcct
    26521 gtaaaccccc ctataccagc gcacacatga aaatctgtgt tgctgccgcc aaaatcatcc
    26581 tcgctgcttc cggcgtggct cgctgttcca aatacgaaaa agagaactat ctccgtatcg
    26641 atttcagcaa agccggtaag gttacatttt acgccgagtt tccaaaaaag atgggcctca
    26701 aaggtaaaaa gctcggcgag tggccggagc tcgttatcca gctggcgcgc gaaaaagcac
    26761 taggtatggc tgacggtgga ctgcgggcag agtccgtaca tgcagcgctg gaaatgtacc
    26821 gggatgaccc aaagccaaag tagcccggca gaagctgagc ccggacagtt tcacaaccta
    26881 cggggtgcgt atcgaccgga ttaaagtaac gttcggcgag cgtgaggtgt tcagcgacgt
    26941 aacatacaat cggctggtgg aagtgctgga cgagtggatc gccactcgct cgaacaataa
    27001 cgccctggag ttgtttgccg agctccgtcg gttctggaag ttctgcgcac ctactctttg
    27061 caacggccgc aatgttgccg ccagtctgcc agatgattat gtttcctccc gcgtacagaa
    27121 acctaccccc acacggcttt ttaccgatat tgaatcaatc gcccgactct ggctcaatgt
    27181 tgctgcctgc acctctgtac accagaagaa tgctgttcgc ttcatgatca tcactggtgt
    27241 tcgtccgatt aatgtccata acctgcgctg ggactacgtt tacgaggagg ctggtgaaat
    27301 tgtttatccg gaaggggtta tcggcatgcg aggggctatg aaaacacaaa aggctttccg
    27361 cctgccgata acgcctgaga tccggcggat tatcgacgag cagaaagcct ggcgtgattc
    27421 agttcctgag tgcaacaggg attatgtatt tttgcagcca cgtgatccaa tgcagccatt
    27481 ttcaaaacga tcactggata agctggtgaa aacatacagc ccggacgggg ctgtaaaatg
    27541 aggtgtactg gcaatagcgg acactaccat ttgttctttt tttaagcagc catctgatga
    27601 t
//
LOCUS       DAZPCS010000036        27474 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZPCS010000036 DAZPCS010000000
VERSION     DAZPCS010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27474
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.036"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            75..1352
                     /gene="waaA"
                     /locus_tag="ACHUQ7_001564"
     CDS             75..1352
                     /gene="waaA"
                     /locus_tag="ACHUQ7_001564"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFR1135032.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            1360..1839
                     /gene="coaD"
                     /locus_tag="ACHUQ7_001565"
     CDS             1360..1839
                     /gene="coaD"
                     /locus_tag="ACHUQ7_001565"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFR1135033.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="ACHUQ7_001566"
     CDS             complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="ACHUQ7_001566"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFR1135034.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="ACHUQ7_001567"
     CDS             complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="ACHUQ7_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFR1135035.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="ACHUQ7_001568"
     CDS             complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="ACHUQ7_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFR1135036.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3426..4094)
                     /gene="radC"
                     /locus_tag="ACHUQ7_001569"
     CDS             complement(3426..4094)
                     /gene="radC"
                     /locus_tag="ACHUQ7_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFR1135037.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            4266..5486
                     /gene="coaBC"
                     /locus_tag="ACHUQ7_001570"
     CDS             4266..5486
                     /gene="coaBC"
                     /locus_tag="ACHUQ7_001570"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFR1135038.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5464..5922
                     /gene="dut"
                     /locus_tag="ACHUQ7_001571"
     CDS             5464..5922
                     /gene="dut"
                     /locus_tag="ACHUQ7_001571"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFR1135039.1"
                     /translation="MMKKIDVKILDPRVRKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            6029..6625
                     /gene="slmA"
                     /locus_tag="ACHUQ7_001572"
     CDS             6029..6625
                     /gene="slmA"
                     /locus_tag="ACHUQ7_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFR1135040.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="ACHUQ7_001573"
     CDS             complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="ACHUQ7_001573"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFR1135041.1"
                     /translation="MKSYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7369..8085)
                     /gene="rph"
                     /locus_tag="ACHUQ7_001574"
     CDS             complement(7369..8085)
                     /gene="rph"
                     /locus_tag="ACHUQ7_001574"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFR1135042.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            8212..9075
                     /locus_tag="ACHUQ7_001575"
     CDS             8212..9075
                     /locus_tag="ACHUQ7_001575"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFR1135043.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMKSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            9296..10120
                     /gene="dinD"
                     /locus_tag="ACHUQ7_001576"
     CDS             9296..10120
                     /gene="dinD"
                     /locus_tag="ACHUQ7_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFR1135044.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTIPEELPTPQVSIKQLENSVKITEKK"
     gene            10411..11028
                     /locus_tag="ACHUQ7_001577"
     CDS             10411..11028
                     /locus_tag="ACHUQ7_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFR1135045.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="ACHUQ7_001578"
     CDS             complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="ACHUQ7_001578"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFR1135046.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVLQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNPLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            12965..13588
                     /gene="gmk"
                     /locus_tag="ACHUQ7_001579"
     CDS             12965..13588
                     /gene="gmk"
                     /locus_tag="ACHUQ7_001579"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFR1135047.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            13643..13918
                     /gene="rpoZ"
                     /locus_tag="ACHUQ7_001580"
     CDS             13643..13918
                     /gene="rpoZ"
                     /locus_tag="ACHUQ7_001580"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFR1135048.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQELEAAELQAVTAIAEGRR"
     gene            13937..16045
                     /gene="spoT"
                     /locus_tag="ACHUQ7_001581"
     CDS             13937..16045
                     /gene="spoT"
                     /locus_tag="ACHUQ7_001581"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFR1135049.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            16052..16741
                     /gene="trmH"
                     /locus_tag="ACHUQ7_001582"
     CDS             16052..16741
                     /gene="trmH"
                     /locus_tag="ACHUQ7_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFR1135050.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            16747..18828
                     /gene="recG"
                     /locus_tag="ACHUQ7_001583"
     CDS             16747..18828
                     /gene="recG"
                     /locus_tag="ACHUQ7_001583"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFR1135051.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="ACHUQ7_001584"
     CDS             complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="ACHUQ7_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFR1135052.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            20347..21738
                     /gene="xanP"
                     /locus_tag="ACHUQ7_001585"
     CDS             20347..21738
                     /gene="xanP"
                     /locus_tag="ACHUQ7_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFR1135053.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            21859..23568
                     /locus_tag="ACHUQ7_001586"
     CDS             21859..23568
                     /locus_tag="ACHUQ7_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFR1135054.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="ACHUQ7_001587"
     CDS             complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="ACHUQ7_001587"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFR1135055.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPIDVQWFDGSDSQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(25949..27332)
                     /locus_tag="ACHUQ7_001588"
                     /pseudo
     CDS             complement(25949..27332)
                     /locus_tag="ACHUQ7_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
BASE COUNT         6707 a         7445 c         6944 g         6378 t
ORIGIN      
        1 aacgggaaaa gtaatggtaa agccacagct aaatacatag aatccccagc acatccataa
       61 gtcagctatt tactatgctc gaattgcttt acaccgccct tctctacctt attcagccgc
      121 tgatctggat acggctctgg gtgcgcggac gtaaggctcc ggcctatcga aaacgctggg
      181 gtgaacgtta cggtttttac cgccatccgc taaaaccagg cggcattatg ctgcactccg
      241 tctccgtcgg tgaaactctg gcggcgatcc cgttggtgcg cgcgctgcgt catcgttatc
      301 ctgatttacc gattaccgtt acaaccatga cgccaaccgg ttcggagcgc gtacaatcgg
      361 ctttcgggaa ggatgttcag cacgtttatc tgccgtatga cctgcccgat gcgctcaacc
      421 gtttcctgaa taaagtcgac cccaaactgg tgttgattat ggaaaccgaa ctatggccta
      481 acctgattgc ggcgctacat aaacgtaaaa ttccgctggt gatcgctaac gcgcgactct
      541 ctgcccgctc ggccgcaggt tatgccaaac tgggtaaatt cgtccgccgc ttgctgcgtc
      601 gtattacgct gattgctgcg caaaatgaag aagatggtgc acgttttgtg gcgctgggcg
      661 caaaaaataa ccaggtaacc gttaccggta gcctgaaatt cgatatttct gtaactccgc
      721 agttggctgc taaagccgtg acgctacgcc gccaatgggc accacaccgc ccggtatgga
      781 ttgccaccag cactcacgaa ggtgaagaga gcgtggtcat tgccgcacat caggcattgt
      841 tacagcaatt cccgaattta ttgctcatcc tggtaccccg tcatccagaa cgcttcccgg
      901 atgcgattaa ccttgtccgc caggctggac taagctatat cacacgctct tcaggggaag
      961 tcccctccac cagcacgcag gttgtggttg gcgatacgat gggcgagttg atgttactgt
     1021 acggcattgc cgatctcgcc tttgttggcg gttcactggt tgaacgtggt gggcataatc
     1081 cgctggaagc tgccgcacac gctattccgg tattgatggg gccgcatact tttaacttta
     1141 aagacatttg cgcgcggctg gagcaggcaa gcgggcttat taccgttacc gatgccacta
     1201 cgcttgcaaa agaggtttcc tctttactca ccgacgccgt ttaccgtagt ttctatggcc
     1261 gtcatgccgt tgaagtactg tatcaaaacc agggcgcgct acagcgtctg cttcaactgc
     1321 tggaacctta cctgccaccg aaaacgcatt gaggttgtta tgcaaaaacg ggcgatttat
     1381 ccgggtactt tcgatcccat taccaatggt catatcgata tcgtgacgcg cgccacgcag
     1441 atgttcgatc acgttattct ggcgattgcc gccagcccca gtaaaaaacc gatgtttacc
     1501 ctggaagagc gtgtggcact ggcacagcag gcaaccgcgc atctggggaa cgtggaagtg
     1561 gtcgggttta gtgatttgat ggcgaacttc gcccgtaatc aacacgctac ggtgctgatt
     1621 cgtggcctgc gtgcggtggc ggattttgaa tatgaaatgc agctggcgca tatgaatcgc
     1681 cacttaatgc cggaactgga aagtgtgttt ctgatgccgt cgaaagagtg gtcgtttatc
     1741 tcttcatcgt tggtgaaaga ggtggcgcgc catcagggcg atgtcaccca tttcctgccg
     1801 gagaatgtcc atcaggcgct gatggcaaaa ttagcgtagc gtttatgccg gatggtatgc
     1861 catccggctc gcatgaatta cttctggcac tgccgacaat aaaacgttgc ccgctgcgca
     1921 tgtttagtcg ccacaatcgg cgtaccgcac acccgacacg gctcaccttt tcgcccgtaa
     1981 acctgcaatt cctgcgcgaa atagcccggt ttaccatcac tttgcagaaa atctttcagc
     2041 gttgtaccac cctgctcaat cgaacgcagc aacaccgctt taatcacccg agctaacaat
     2101 tcacactctg ccagcgacag tgatgacgcc agccgatccg gatggatccc cgccgcaaac
     2161 agtgattcgc tggcatagat attccctacc cctaccacca gcttgttatc catcagccac
     2221 ggtttaatcg ccgttttttt cttcgcgcac ttctgatgca gatactcacc attgaaatcg
     2281 tcgctaagcg gctccggtcc aagatgggcc agcacattat gcccttccag ctctttggtc
     2341 cacagccagg caccaaagcg gcgcggatcg gtgtagcgca gcactttacc gttgctcatc
     2401 accaaatcca catggtcatg cttttcaggg ggaagttctt ctggaaggat gcgcaggctg
     2461 ccagacatcc ccaaatgaat gataatccag ccctcaggca gctccagcag cagatattta
     2521 gcccgccgct gcacgctaag cactggttga tcgcttaatc ggtagatctc ttctgaaacc
     2581 ggccagcgca agcgtccgtt gcgcaccacc gcatgaagaa tggttgcacc aacgagatgc
     2641 ggttctatgc cgcgacggct ggtttcaact tcgggtaatt caggcatagc atctccagga
     2701 atgaacagat gcaatcaata tgggggcaag cagataacaa aaaaccccgc cggagcgagg
     2761 tttttcgtta catcaaagcg agaattattt gattttcgct tctttgtaga tcacgtgctg
     2821 acgaacaact ggatcgaatt ttttcagttc cagtttttcc ggcttagtac gtttgttctt
     2881 cgtagtggta tagaagtgac cagtaccagc agaagaaacc agcttgattt tctcacgaat
     2941 acctttagcc atgatttatt tcctctaagt acttagtact tttcgccacg ggcacgcagt
     3001 tcagccagaa ctgtatcgat gcctttttta tcgattacac gcataccttt agcagatacg
     3061 cgcagggtga caaaacgctt ctcgctctca acccagaaac ggtgagagtg caggttcggc
     3121 aggaaacggc gtttagtcgc gttcagtgcg tgggaacggt tgttaccggt caccggacgc
     3181 ttgccagtaa cttggcagac tcgggacatg tctattctcc aaaaatcaaa ttagctcgag
     3241 cttcgtatgg ggtattggcg cctcgtcagg ctttacagcc cggtcatcgc agttctatgt
     3301 gaactctcga ttgccaggcc caaatgccaa acccgagatt ctcaaaggtg gcgtagtata
     3361 cgctgactca gcgatgtgct caagtcccga acagacaaag atcccgaagg atcgcgcata
     3421 gcgggttaaa tccagccgcg ttcggcaaaa gaaacatact ctccacgccc aatcacgata
     3481 tggtcgagca cgcgtaaatc catgaactga caactcttta ttatccgttc agtaatgagt
     3541 ttatccgctt tactgggttc agcacaaccc gaagggtgat tatgtgcaag gatcagcgcc
     3601 gaggcgttta tttttatcgc ttcgcggata atttcccgag gatggacttc aacatggttt
     3661 agcgtgccgg aaaaaagacg gctatgcgtt ataacccggt gttgtgagtc gagaaagatc
     3721 accataaaga tctcccgctc ctcacccgtg agctggcttt gtaaaaattc ccgcgtcatc
     3781 tccgggctga gtaaagggct ttcttcacgc atccgcacgt tgtagtaacg tcgcgccagt
     3841 tcagcaatcc cctttaactg ggcaaatttc gctacgccaa ttccatgaac accactaaat
     3901 tgttcatatt cagaggttaa caagccataa agagagccga aattctccag catctctttt
     3961 gccagggtta atacatcttt accgcgcgtt ccggtacgca gaaatagcgc cagcagctcg
     4021 acatccgtta aggcgctaat accaaacttc agcatttttt cgcgcggcat caacagctgt
     4081 gcattgtttt tcaccttcac ctcctttgtg gtgctcgcat cctgtcatag ccgttccgtt
     4141 aaatcgacgg ccagttttca atcctggaaa gagcctcgca aagtgaatct caggtgaagg
     4201 cacgacaaca aaagtattgt gataaaatcg ccaacttctg gtgtcaccca acaggaaaaa
     4261 tcatcatgag cctggccggt aaaaaaatcg ttctcggcgt tagcggcggt attgctgcct
     4321 ataaaacccc tgaactggtg cgtcgtttgc gcgatcgcgg ggccgacgtc cgcgtagcca
     4381 tgaccgaagc ggcaaaagcc tttatcaccc cacttagctt gcaggcggtt tctggttatc
     4441 ccgtttccga cagtctgctt gacccggcag ccgaagccgc tatgggccat attgagctgg
     4501 gtaaatgggc tgatttagtg attctcgccc ctgccacggc agatttgatt gcccgtgttg
     4561 ctgccggaat ggcgaatgac ctggtatcga cgatttgtct ggctacacct gcgcctgtag
     4621 ccgtgctccc cgccatgaac cagcagatgt accgtgccgc tgccacgcag cataatttag
     4681 aggtgcttgc ttcccgtggt ttgctcatct gggggccaga cagtggcagt caggcttgtg
     4741 gtgatatcgg tcctggacga atgctcgatc cgttaaccat tgtggatatg gcggtggcgc
     4801 atttttcgcc cgtcaacgac ctgaaacatc tgaacattat gattaccgcc ggtccgacgc
     4861 gtgaaccgct cgatccggtg cgttatatct ctaatcacag ctccggcaag atgggttttg
     4921 ctatcgccgc cgctgctgcc cgtcgtggcg cgaacgtcac gctggtatca ggtccagttt
     4981 cactaccgac gccaccgttt gttaaacgtg ttgatgtgat gaccgcgctg gaaatggaag
     5041 ccgccgtgaa tgcttctgta cagcagcaaa atatttttat cggttgcgcc gccgtggcgg
     5101 attatcgcgc agctaccgtg gccccagaaa aaatcaaaaa gcaggccacg cagggtgatg
     5161 aattaacaat aaaaatggtt aaaaaccccg atatcgtcgc aggcgttgcc gcactaaaag
     5221 accatcgacc ctacgtcgtt ggatttgccg ccgaaacaaa taatgtggaa gaatacgccc
     5281 ggcaaaaacg tatccgtaaa aaccttgatc tgatctgcgc gaacgatgtt tcccagccaa
     5341 ctcaaggatt taacagcgac aacaacgcat tacacctttt ctggcaggac ggagataaag
     5401 tcttaccgct tgagcgcaaa gagctccttg gccaattatt actcgacgag atcgtgaccc
     5461 gttatgatga aaaaaatcga cgttaagatt ctggacccgc gcgttaggaa ggaatttccg
     5521 ctcccgactt atgccacctc tggctctgcc ggacttgacc tgcgtgcctg tctcgacgac
     5581 gccgtagaac tggctccggg tgacactacg ctggttccga ccgggctggc gattcatatt
     5641 gccgatcctt cactggcggc aatgatgctg ccgcgctccg gattgggaca taagcacggt
     5701 atcgtgcttg gtaacctggt aggattgatc gattccgact atcagggcca gttgatgatt
     5761 tccgtgtgga accgtggtca ggacagcttc accattcaac ctggcgaacg catcgcccag
     5821 atgatttttg ttccggtagt acaggctgaa tttaatctgg tggaagattt cgacgccacc
     5881 gaccgcggtg aaggcggctt tggtcactct ggtcgtcagt aacacatacg catccgaata
     5941 acgtcataac atagccgcaa acatttcgtt tgcggtcata gcgtgggtgc cgcctggcaa
     6001 gtgcttattt tcaggggtat tttgtaacat ggcagaaaaa caaactgcga aaaggaaccg
     6061 tcgcgaggaa atacttcagt ctctggcgct gatgctggaa tccagcgatg gaagccaacg
     6121 tatcacgacg gcaaaactgg ccgcctctgt cggcgtttcc gaagcggcac tgtatcgcca
     6181 cttccccagt aagacccgca tgttcgatag cctgattgag tttatcgaag atagcctgat
     6241 tactcgcatc aacctgattc tgaaagatga gaaagacacc acagcgcgcc tgcgtctgat
     6301 tgtgttgctg cttctcggtt ttggtgagcg taatcctggc ctgacccgca tcctcactgg
     6361 tcatgcgcta atgtttgaac aggatcgcct gcaagggcgc atcaaccagc tgttcgagcg
     6421 tattgaagcg cagctgcgcc aggtattgcg tgaaaagaga atgcgtgagg gtgaaggtta
     6481 caccaccgat gaaaccctgc tggcaagcca gatcctggcc ttctgtgaag gtatgctgtc
     6541 acgttttgtc cgcagcgaat ttaaataccg cccgacggat gattttgacg cccgctggcc
     6601 gctaattgcc gcgcagttgc agtaatatga cgccggatga cttttcatcc ggcgagtttc
     6661 tttaaacgcc aaactcttcg cgataggcct taaccgccgc cagatgttcc gccatttccg
     6721 gcttctcttc cagataggca atcagatctt tcagggtgat gatagagatc actttgcagt
     6781 tgtaatcacg ctcaacttcc tgaatcgccg aaatctcgcc gcgcccgcgt tcctgacgat
     6841 cgagcgaaat caacacgcca gcaagcgtcg cgccattggc ctgaataatc tccatcgact
     6901 cgcgaatcgc cgttccggcg gtgatcacat catctaccag cattacgcgt ccttgtaacg
     6961 cgctaccaac cagattgccg ccttcaccgt ggtcttttgc ttctttgcgg ttaaagcagt
     7021 acggcaggtc caggtcgtga tgctccgcca gtgccacagc ggttgtggtg gcaatcggga
     7081 tccctttgta agcagggcca aacagcagat cgaactcaat gccggaatcc accaacgctt
     7141 cagcgtaaaa acggcctaac agtgccagat cgcgcccggt attaaacagc ccggcgttga
     7201 agaaataggg gcttttgcgc ccggatttca gcgtaaactc gccaaacttt aacacctgct
     7261 tgctaagcgc aaattcaata aactggcgct gatatgattt catgccttcg ctcctcatct
     7321 tacttttcta cagacaaaaa aaaggcgact catcagtcgc cttaaaaatc agtttgccag
     7381 cgccgccttc tgcgtcgcta caatggattc gattccccct cgggccagcg ccaacaaggt
     7441 gagtagctct tcatgggtga acggctcgcc ttctgccgtc ccctgcactt caatgatgcg
     7501 cccgtcttcg gtcatcacta cgttcatgtc ggtctctgcg gctgagtctt caacgtattc
     7561 cagatcgcaa attgcttcac cgtttacaat accgacagaa actgcggcta ccatcccttt
     7621 catcgggttg gttttcagct tgccgttttc caccagcttc tgtagcgcat ctgccagcgc
     7681 cacgcaggca cccgtaatcg acgcggtacg agtgccacca tcagcctgaa gcacgtcgca
     7741 gtccagcgta atggtgaact cacccagcgc tttcaaatct actgccgcgc gaagagcacg
     7801 ggcgatcaga cgctggattt ccattgtgcg tccaccctgc ttacctttcg ccgcttcacg
     7861 agcgttacgg gtgtgggtag aacgtggcag catgccgtac tctgcggtga tccagccctg
     7921 gccctgacct ttcaggaagc gcggcacgcc ttcttcaata gaggcggtac acaatacttt
     7981 ggtatcgcca aattcgacca gcaccgagcc ttctgcatgt tttgtatagt tacgagtcag
     8041 ggtaacggga cgcacctgat tattgctacg gcctgctgga cgcatattga aatctccggc
     8101 ttgaaacaaa tgtggctgcg cattatacgg acttccggcg gttattccta tcctgacaag
     8161 gcatcgatgg ctataatcct tccacctctc cttttataaa caggaacgtc tatgatccgc
     8221 agtatgaccg cctacgcccg gcgtgaaatc aagggtgaat gggggagcgc aacctgggaa
     8281 atgcgctcgg taaaccagcg ttatctggaa acttactttc gtctgccgga gcagttccgt
     8341 agccttgaac ctgtcgttcg cgagcgtatt cgttctcgcc tgacgcgcgg taaagtggaa
     8401 tgtaccctgc gctatgagcc agatgttagc gcacaaggtg agctgatcct caatgaaaaa
     8461 ctggctaaac agctggtaac tgccgcgaac tgggtaaaaa tgaagagtga cgaaggggaa
     8521 atcaacccgg ttgatattct acgctggccg ggcgtgatgg cagcccagga gcaggatctc
     8581 gacgcgattg ccgctgaaat tctcgcggcg ttggatggta cgctggacga ctttatcgtc
     8641 gcgcgcgaaa ccgaaggtca ggcactgaaa gcattgatcg agcagcgtct ggaaggcgtc
     8701 accgccgaag tggtcaaagt ccgcgcccat atgccggaaa ttctgcaatg gcagcgtgag
     8761 cgtctggtcg cgaagctgga agatgctcag gttcagttgg aaaacaaccg tctggagcag
     8821 gagttggttc tgctggcaca acgaattgac gttgccgaag agctggaccg cctcgaagcg
     8881 catgttaaag agacgtacaa cattctgaag aaaaaagaag cggttggtcg tcgtctggac
     8941 tttatgatgc aggagttcaa ccgtgagtcg aacactcttg cgtcgaaatc tatcaatgcc
     9001 gaagtgacaa actccgccat cgagctgaaa gtgttgattg agcagatgcg cgagcagatt
     9061 cagaatattg agtaactcgt aacaccaatt ctgacttttc agccttcaca aatgccgccc
     9121 actcaaacag agcggcattt ttcttccccg caacttcaat tctgttttgc gtgcctgctc
     9181 cagattttgc gatgtttttt tgcccagcac actgagaacg tgagatactc acaactgtat
     9241 ataaatacag ttacagattt actttctttg caatcgatat cacatggagt gggcaatgaa
     9301 cgaacatcat caaccttttg aagagataaa actgattaat gcaaacggag cagaacaatg
     9361 gtcagcaaga caacttggga aactactggg ttattcagag tatcgtcact ttatacctgt
     9421 attaacgcgc gccaaagagg cctgtgagaa cagtggtcac acaattgatg accatttcga
     9481 ggagatcctc gatatggtca aaattggctc aaatgccaaa agagcattaa aagacatcgt
     9541 actctcccgc tatgcctgtt acctggtagt acaaaacggc gaccccgcga aaccggtcat
     9601 tgcggcaggg cagacttatt ttactatcca gacccgacgg caggagcttg ctgacgatga
     9661 agcatttaag caacttcgtg aagacgaaaa gcgtctgttt ctgagaaatg agttgaaaga
     9721 acataacaaa caattggttg aggcagcaca gcaagctggg gtagcgacag ctactgattt
     9781 cgccatattt cagaatcatg gttaccaggg gctgtatggt ggattagatc agaaagccat
     9841 ccaccaactg aaggggctga aaaagagtca gaagatcctg gatcatatgg gttcaacaga
     9901 actggcggct aatctctttc gagctaccca aacagaagaa aaactcaagc gggatagcgt
     9961 gaattcaaaa cagcaagcta ataccacaca ctttgacgtg ggtagcaagg tgaggcaaac
    10021 cattcaggaa cttggcggaa ccatacctga ggagttgccg accccgcagg tcagcatcaa
    10081 gcagttggaa aacagtgtaa aaattacaga gaagaaatag cagaaacgaa aaactctttt
    10141 tgttgattga gacacccgat gcgtaagcca aggtccaggt gcagtgaaca caatggctaa
    10201 acattgcacc tttcttttcc cccctcagtt ttaacctatt ttttcttatg cattttctca
    10261 gacaagaagt cagaagaatg catctacgct acagaaaata gcgatttcac ataactaaaa
    10321 gttatcaatt tcctctcacc ttaaaaaata tcaatcgtga caatgcgcac aaatcgctac
    10381 cctgccagac agatttttag ggagagaacc atgctgttac acattttgta tttggttggc
    10441 attactgccg aagccatgac gggggcgctg gcggccgggc gacggcgcat ggatacattt
    10501 ggcgtaatta ttattgcaac cgccaccgca attggcggag ggtcagtgcg cgatattctg
    10561 ctgggccact atccgctcgg ctgggtcaaa cacccggaat atgtgattat cgtcgctacc
    10621 gccgcagtac tcaccactat cgttgctcct gtaatgcctt acctgcgcaa agtgtttctg
    10681 gtgctcgatg cgctcggact ggtggtcttt tctatcatcg gcgcacaggt tgcactggat
    10741 atggggcacg gcccaattat tgccgttgtc gcggcggtaa ccaccggtgt atttggcggc
    10801 gttttgcgcg atatgttctg taaacgcatt ccgctggtct ttcaaaaaga gttgtatgcc
    10861 ggtgtctcat ttgcctccgc cgtgctgtac atcgcgctgc aacactatgt ttctaaccat
    10921 gatgtggtga ttatctccac cctggtattc ggcttttttg ctcgtttact ggcgctacgt
    10981 cttaagctgg gattaccggt cttttactac agccacgaag gtcactaagg ttcaaaacct
    11041 gtgatctgct gggcagccag ccaactgccc agcttcttga tttgcgcatt ttccttccat
    11101 tcaataacct gtctggcgcg cccagatcca gtccccggca gctgctgcca gaactgctcc
    11161 gtgctaaata aaagttgcga ccaggaccgt tcatcactgg cattaagcgc cgcccgggtt
    11221 agcggtattc ccattgccat cacccagcga gtaaaaggct ggttacgagc cagattaaac
    11281 tgatgccata actgcgcact tttacttttc gcgatccccg gcgtgttctg taattgctct
    11341 ggcgttaata aaagccagga aaagatatgc tcaaagcgat gagtctgatg cagcgcgcgc
    11401 caaccggcct caccaatgcc atccagcccc agaacctgtt ttgaccccag ccagactaag
    11461 cgtgaaatga actgttcctg acaaacatca gaagcaaagt agcaggtcaa cgggttaaag
    11521 cggttttctg gtggtgtcgg ttttgtacgt tctgcaccgc gccagaccac atcatcaatg
    11581 cgaggaatac cctgaccggc aaggctgacg aggatctgat caccaggcgc aatatcccac
    11641 tcctgccagc gcctgacgga accaatattc acccgctgga cttttttatc atccagcatg
    11701 acaggtgcga gtgacgcaac caccgatatt ttaccgctct tacccaccgc aaactgaatc
    11761 gccttcactt cggcaacctg agctacaggt tgatatttcc aggccaccag ccactccgcc
    11821 tggcccggta gccaatgacg ggattcgggt tccttagccc ctcgcacaac cacgccatcg
    11881 gtgacgaagg gtaatttcgc tttccaccac tcattgcgta cgcgcgcaac ctcatcagca
    11941 tttttaaccg cacgggtata cctctgcgtt agagtaaaac ctgcggtagc cagctccttt
    12001 aaacgatcgg tcattaactg cggtccatcc ggccatgccc agacaaaaac agccagtgag
    12061 tttagcgtgt cgctattccc ctggcgcatc atcaggccag caacttttgc gcgggcattt
    12121 attcccccca tttgttgttg gatatgcccc tcgcgctgga gaaatatttc cccctgaagc
    12181 gtactgttgg ctaaaggccc gctaacggtt tgcagcacag cggaaattaa gctaactttc
    12241 tgcgtccagt cctcgccttt caggccatta ccgcgactga ttgctttgtt cagtttcccg
    12301 tcccgataaa ccagggttac cgcaacgcca tcaacttttg gctgcaccca aagatcgcta
    12361 cgttctcgca tccacagact taatgcgttt ttatccgcca ttttgcgtac acccgtatgg
    12421 gcaaccggat gcataaccgc accatttaaa ggcggcatca tcacgtcgcg gggctcgctc
    12481 acaaagcagc gttgccactg cgtaagacgg gcgcttaact gatcgtaaac accgtcttcc
    12541 acctcacttt ttccttcctt ccagtagtca tcgtcccact gttttatttg ctgttgcagg
    12601 cgggaaattt cttcctgtgc tctggctggc gaccaggccg gacagaccgc ccacacagat
    12661 gattgccagc acaagatact tattaatatc gccatccata ctttcatctt cacctccgct
    12721 gtagtcaggc tggcagatat acaacgtggt gaaggcaaag ccgagtggca aaaacggagt
    12781 ctgcgaggac gcttcctgag aatcgtcttt attgcagtaa atcacaggca aatgcggaaa
    12841 cagctacgca aaacgcaaca actttgcgca aaaagtgtga gcaagggcta cgtcacatgg
    12901 ccgcgccgtg tataataagc tcgtatgtag gctttatttc gctaatcaca tacgaaagat
    12961 actcatggct caaggcacgc tttatattgt ttctgccccc agtggcgcgg gtaaatccag
    13021 cctgattcag gctttattaa aaacccaacc gttgtatgac acccaggttt ctgtttcaca
    13081 caccacacgc caaccgcgtc ctggtgaagt ccacggtgaa cattatttct ttgttaatca
    13141 tgatgaattt aaagaaatga ttagcagaga tgcgttcctc gaacacgcag aagtttttgg
    13201 taattactat ggcacttcgc gtgaggccat tgagcaagta ctggcgaccg gtgtcgatgt
    13261 ttttctcgat atcgactggc agggcgcgca gcaaattcgc cagaagatgc cgcacgcgcg
    13321 gagtatcttt attttaccgc cgtccaaaat tgaactggac cgccgtctac gcggtcgcgg
    13381 tcaggacagc gaagaggtca ttgcaaagcg tatggcgcaa gctgttgcag aaatgagcca
    13441 ttacgccgaa tatgattatc tgattgtgaa tgatgacttc gataccgcgt tgaccgattt
    13501 gaagaccatt attcgcgccg aacgtctgcg catgagccgc caaaagcagc gtcatgacgc
    13561 tttaatcagc aaattgttgg cagactgaac ctgatttcag tatcatgccc agtcatttct
    13621 tcacctgtgg agctttttaa gtatggcacg cgtaactgtt caggacgctg tagagaaaat
    13681 tggtaaccgt tttgacctgg tactggtcgc cgcgcgtcgc gctcgtcaga tgcaggtagg
    13741 cggaaaggat ccgctggtac cggaagaaaa cgataaaacc actgtaatcg cgctgcgcga
    13801 aatcgaagaa ggtctgatca acaaccagat cctcgacgtt cgcgaacgcc aggaacagca
    13861 agagctggaa gccgctgaat tacaagccgt taccgctatt gctgaaggtc gtcgttaatc
    13921 acaaagcggg tcgcccttgt atctgtttga aagcctgaat caactgattc aaacctacct
    13981 gccggaagac caaatcaagc gtctgcggca ggcgtatctc gttgcacgtg atgctcacga
    14041 ggggcaaaca cgttcaagcg gtgaacccta tatcacgcac ccggtagcgg ttgcctgcat
    14101 tctggccgag atgaaactcg actatgaaac gctgatggcg gcgctgctgc atgacgtgat
    14161 tgaagatact cccgccacct accaggatat ggaacagctt tttggtaaaa gcgtcgccga
    14221 gctggtagag ggggtgtcga aacttgataa actcaagttc cgcgataaga aagaggcgca
    14281 ggccgaaaac tttcgcaaga tgattatggc gatggtgcag gatatccgcg tcatcctcat
    14341 caaacttgcc gaccgtaccc acaacatgcg cacgctgggc tcacttcgcc cggacaaacg
    14401 tcgccgcatc gcccgtgaaa ctctcgaaat ttacagcccg ctggcgcacc gtttaggtat
    14461 ccaccacatt aaaaccgaac tcgaagagct gggttttgag gcgctgtatc ccaaccgtta
    14521 tcgcgtaatc aaagaagtgg tgaaagccgc gcgcggcaac cgtaaagaga tgatccaaaa
    14581 aatcctctcg gaaatcgaag ggcgtttgca ggaagcggga attccctgcc gcgtcagtgg
    14641 tcgcgagaag catctttatt cgatttactg caaaatggtg ctcaaagagc agcgttttca
    14701 ctcaatcatg gacatctacg ctttccgcgt gatcgtcaat gattccgaca cctgttatcg
    14761 cgtgctcggg caaatgcata gcctgtacaa gccgcgtccg ggtcgcgtga aagactacat
    14821 cgccattcca aaagcgaacg gctatcagtc tttgcacacc tcgatgatcg gcccgcacgg
    14881 tgtgccggtt gaggtccaga tccgtaccga agatatggat cagatggcgg agatgggtgt
    14941 tgccgcgcac tgggcttata aagagcacgg cgaaaccagc accaccgcgc aaatccgcgc
    15001 ccagcgctgg atgcaaagcc tgctggagct gcaacagagc gccggtagtt cgtttgaatt
    15061 tatcgagagc gttaaatccg atctcttccc ggatgagatt tacgttttca caccggaagg
    15121 gcgaattgtc gagctgcctg ccggtgcaac gcccgtcgac ttcgcttatg cagtgcatac
    15181 cgatatcggt catgcctgcg tgggcgcacg cgttgaccgc cagccttacc cgctgtcgca
    15241 gccgcttacc agcggtcaaa ccgttgaaat cattaccgca ccgggcgctc gcccgaatgc
    15301 cgcgtggctg aactttgtcg ttagctcaaa agcgcgcgcc aaaattcgtc agttgctgaa
    15361 aaacctcaag cgtgatgatt ctgtaagcct gggccgtcgt ctgctcaacc atgctttggg
    15421 tggtagccgt aagctgaatg aaatcccgca ggaaaatatt cagcgcgagc tggatcgcat
    15481 gaagctggca acgcttgacg atctgctggc agaaatcgga cttggtaacg caatgagcgt
    15541 ggtggtcgcg aaaaatctgc aacatgggga cgcctccatt ccaccggcaa cccaaagcca
    15601 cggacatctg cccattaaag gtgccgatgg cgtactgatc acctttgcga aatgctgccg
    15661 ccctattcct ggcgacccga ttatcgccca cgtcagcccc ggtaaaggtc tggtgatcca
    15721 ccatgaatcc tgccgtaata tccgtggcta ccagaaagag ccagagaagt ttatggctgt
    15781 ggaatgggat aaagagacgg cgcaggagtt catcaccgaa atcaaggtgg agatgttcaa
    15841 tcatcagggc gcgctggcaa acctgacggc ggcaattaac accacgactt cgaatattca
    15901 aagtttgaat acggaagaga aagatggtcg cgtctacagc gcctttattc gtctgaccgc
    15961 tcgtgaccgt gtgcatctgg cgaatatcat gcgcaaaatc cgcgtgatgc cagacgtgat
    16021 taaagtcacc cgaaaccgaa attaatgttt tatgaaccca acacgttatg cacgcatctg
    16081 cgaaatgctc gccaggcggc agcctgatct gaccgtctgc atggagcagg tccacaaacc
    16141 tcataacgtt tctgcgatta ttcgtaccgc agatgccgtt ggcgtacatg aagttcacgc
    16201 cgtctggcct ggtagccgca tgcgcaccat ggcttcggca gcggcgggta gtaacagctg
    16261 ggtacaggtg aaaacacacc gcaccattgg cgatgccgtc gctcatctca aaggccaggg
    16321 catgcagatt ctggcaaccc atctttctga taatgctgtc gatttccgcg aaattgatta
    16381 cactcgcccg acctgcattt tgatgggaca ggagaaaacg ggcatcacgc aggaagcatt
    16441 ggccctggcg gatcaggaca tcatcattcc gatgatcggc atggtgcagt cgctgaatgt
    16501 ttccgttgcc tcagccctca ttctttacga agcccagcgt cagcggcaaa atgcaggcat
    16561 gtacctgcgt gaaaacagca tgttgccgga agcagagcaa caacgcctgt tgtttgaagg
    16621 cggctatccg gtgctggcga aagtcgcaaa acgcaaaggc ctgccttatc cccacgtcaa
    16681 tcagcaaggc gagatcgaag ctgatgccga ctggtgggct actatgcagg ctgcagggta
    16741 agtgccatga aaggtcgcct gttagatgct gtcccactca gttccctaac gggcgttggc
    16801 gcagcactta gtaacaaact ggcgaaaatc aacctgcata ccgtgcagga tctactctta
    16861 caccttcccc tgcgctacga agatcgcacc catctctacc ccatcggaga actactgccg
    16921 ggcgtttatg ccacggtgga aggcgaagtg ctgaactgca atatctcctt cggcggtcgg
    16981 cggatgatga cctgccagat cagcgacggt tccggcatcc tcaccatgcg ctttttcaat
    17041 ttcagcgcgg caatgaaaaa tagcctggcg acgggccgcc gtgtactggc ttatggcgaa
    17101 gcaaagcgcg gtaaatatgg tgcggagatg atccacccgg aataccgcgt gcagggcgat
    17161 ctcagcacgc cagaattaca ggaaacgctc acgccggttt atccaacaac ggaaggcgta
    17221 aagcaggcca cgctgcgtaa attaaccgac caggcgctgg atctgctcga cacctgcgcc
    17281 attgaagaac tcctgccgcc ggaactgtca caaggaatga tgacgctacc ggaagcgttg
    17341 cgcactttgc accgcccgcc accgacgcta cagcttagcg atctggaaac cgggcagcat
    17401 ccggcgcaac gtcgtctgat tctggaagaa ctgctggcgc acaacctcag catgttagcc
    17461 ttacgtgccg gagcacagcg ttttcatgcc cagccgctga gcgccaatga cacgctgaaa
    17521 aataaactcc tcgccgcctt accgttcaag ccaacgggcg cacaggcacg cgtagtggcg
    17581 gagatcgagc atgatatggc gctggatgtg ccgatgatgc gcctggtaca gggcgatgta
    17641 ggttccggta aaacgctggt cgccgccctc gccgcgctgc gtgcgattgc ccacggcaaa
    17701 caggtggcac tgatggcacc aaccgaatta ctcgccgagc agcacgccaa taacttccgc
    17761 aactggtttg caccgctcgg tatcgaagtg ggctggctcg ccggtaagca gaaaggcaaa
    17821 gcgcgactgt ctcagcagga agccatcgcc agcggtcagg tgcagatgat tgtcggtaca
    17881 cacgccatct tccaggaaca ggtgcagttt aacggcctgg cgctggtgat tatcgacgaa
    17941 cagcatcgtt ttggcgtgca tcagcgtctg gcattgtggg agaaaggcca gcagcagggc
    18001 ttccatccgc atcagttgat catgaccgcc acgccgatcc cccgcacgct ggcaatgacg
    18061 gcgtatgccg atctcgatac ctcggtgata gatgagctgc cgccaggccg cacgccagtg
    18121 actacggtcg ctattcctga tacccgccgt accgacatca ttgaccgcgt gcgccacgcc
    18181 tgcataactg aaggtcgtca ggcgtactgg gtttgtacgt tgattgaaga gtcagagtta
    18241 ctggaagcgc aggcggcgga agctacctgg gaagagttga aactggcgct accagaattg
    18301 aatgttggcc tggtacacgg gcggatgaaa ccagccgaga aacaggcggt gatggcgtcg
    18361 tttaaacaag gtgagctaca tctgctggtt gccactaccg ttattgaagt cggtgttgat
    18421 gtgcctaacg ccagcctgat gattatcgaa aaccctgagc gtctgggtct ggcgcagtta
    18481 caccagctgc gcgggcgcgt aggtcgtggc gcggtggctt ctcactgcgt gctgctctac
    18541 aaaacgccgc tttctaaaac ggcgcaaatt cgcctgcaag tgctgcgcga cagtaacgac
    18601 ggttttgtga ttgcgcaaaa agatctggag attcgcggcc ctggcgaatt gttaggcacg
    18661 cgtcagacgg gtaatgctga atttaaagtg gcggatttac tgcgcgatca ggcgatgatc
    18721 ccggaagttc agcgcctggc gcgacatatt cacgaacgtt acccacaaca ggcaaaagcc
    18781 ttgatagaac gctggatgcc ggagacagaa cgttactcga atgcgtaaaa ggcggcggtg
    18841 agaagaccgc cgcttcatcg gttaaccggc aaaaatcggc aacattaaat acagcttaat
    18901 caccagcgca ttgacgatat cgataaagaa cgcaccgacc atcggcacca ccaaaaacgc
    18961 catgtgcgac gggccaaagc gttcagtgat cgcctgcatg ttggcgattg ccgttggcgt
    19021 tgcaccgagg ccaaaaccac agtgacccgc agccagcact gccgcatcgt agtttttgcc
    19081 catcatgcgc caggtaacga agatggcata caacgccatg aagatggtct gtaccaccag
    19141 aatcgccagc atcggcagcg ccagcgaagc cagctcccac agtttcagcc ccatcaacgc
    19201 catcgccagg aacaacgaca agcttacgtt acccagcacg gataccgcac gctcaaagac
    19261 gcggtaaaag cccattattg acagaccgtt gctcagaatc acgccaacaa acagtacaca
    19321 gacgaaggtc ggcagttcaa aagcagtgcc agccaaaagt tgcgcaacaa ttttccccac
    19381 cgtcaggcag atagcaatca gcgcgatagt ttcaatcagc accaacgagg tgatcatgcg
    19441 tcccacatcc ggcttttcaa acgccgtcgg gacttcctgg tcatccggaa taccgttcgg
    19501 cgtggtggag tgtttcacca gatagcgcgc caccggaccg ccaatcaagc cgcccagcac
    19561 cagaccgaac gttgcacagg ccatcgccac ttccgtcgca ttggtgaagc cataacgttc
    19621 aatgaacaat ttactccacg cagcgcccgt accgtgaccg ccggaaagcg taatagaacc
    19681 ggccaacagc cccatcagcg gatcaagccc taacaggcta gccataccaa tgccaatggc
    19741 attttgcatc accaacagac caacaaccac aatcaagaag atgccaacca cacgcccacc
    19801 ggcacgcaaa ctggcaatgt tggcgttcag gccaatggtg gcgaagaaag ccagcattaa
    19861 cggatcgcgc agggacatat caaagttgac ttcccagccc atgctttttt tcagtactag
    19921 tagcgccagc gccaccaaca aaccacccgc aacaggttcc ggtatggtgt atttcttcaa
    19981 aaaggagacg gaatggacca acttacgccc gagcagcaac gtcagcgttg cggcaacaag
    20041 cgttgctaaa gtatcgagat gaaacatagt tactcctttg tatccgcatc actctttcat
    20101 actcgtcata cttcatcgcg cagtggcaac ttcatgaact atgacgggta tcgagttatg
    20161 ctttcctggg tcaaattctc agctgttaat gaacaaatgg catggatttt aagcagaaaa
    20221 ataccaaaag ttatataaaa aagtgcattt atctcattta aatatttaga tgaggatgtg
    20281 gcaaacgttt gcttttctct ttcggtcagc taaaatgccc gctttgctac cacgggattg
    20341 ttttcgatgt ctgtttccac cctcgagtca gaaaatgcgc aaccggttgc tcagactcaa
    20401 aacagcgaac tgatttaccg tcttgaagat cgtccgccgc ttcctcaaac cctgtttgcc
    20461 gcctgtcagc atctgctggc gatgttcgtt gcggtgatca cgccagcgct attaatctgc
    20521 caggcgctgg gtttaccggc acaagacacg caacacatta ttagtatgtc gctgtttgcc
    20581 tccggtgtgg catcgattat tcaaattaag gcctggggtc cggttggctc cgggctgttg
    20641 tctattcagg gcaccagctt caactttgtt gccccgctga ttatgggcgg taccgcgctg
    20701 aaaaccggtg gtgctgatgt tcctaccatg atggcggctt tgttcggcac gttgatgctg
    20761 gcaagttgca ccgagatggt gatctcccgc gttctgcatc tggcgcgccg cattattacg
    20821 ccgctggttt ctggcgttgt ggtgatgatt atcggcctgt cgctaattca ggtggggctg
    20881 acctccattg gcggcggtta cgcagccatg agcgataaca ccttcggcgc accgaaaaat
    20941 ctgctgctgg caggcgtggt tttagcctta attatcctgc ttaaccgtca acgtaaccct
    21001 tacttacgcg tggcctcact ggtaattgcg atggcggccg gatatgcgct ggcgtggttt
    21061 atgggcatgt tgccagaaag caacgaaccg atgacgcaag aactgattat ggtgccaacg
    21121 ccgctctatt acggtcttgg cattgaatgg agtctgctgc tgccgctgat gctggtcttt
    21181 atgatcactt cgctggaaac cattggcgat atcacggcga cctctgacgt ttccgaacag
    21241 ccagtgtccg gtccgctgta catgaaacgc ctgaaaggcg gcgtgctggc aaacggcctg
    21301 aactcgtttg tttcggcggt gtttaacacc ttcccgaact cctgcttcgg gcaaaacaac
    21361 ggagtgatcc agttgactgg tgttgccagc cgctatgtcg gttttgtcgt cgcgctgatg
    21421 ttgatcgtgc tgggtctgtt cccggcagtg agcggttttg tacaacacat tccagaaccg
    21481 gttctgggcg gcgcaacgct tgtaatgttt ggcaccatcg ccgcctccgg tgtgcgtatc
    21541 gtttctcgtg agccgctgaa ccgtcgggcg attctgatta tcgcgctgtc gctggcggtt
    21601 ggtctgggcg tgtctcagca gccgctgatt ttgcagtttg cccctgaatg gctgaaaaac
    21661 ctgctctcct ccgggatcgc cgcgggcggt attactgcca tcgtgctgaa tctgattttc
    21721 ccaccagaaa aacagtaatt cactggcggc ggtaagataa ttaccgccgc agtctcacct
    21781 ctttcaccat tcccgccttg aggattgcgc gtaaatcgtg cataactccc ttatgtgcat
    21841 ttcacgggat ggaagaccat gaaatttatt gggaagctgc ttctctacat tctcatcgct
    21901 ctgttagtgg tgatcgctgg cctctatttt cttctgcaaa cccgctgggg agcagaacat
    21961 atcagcgcat gggtttccga gaatagcgac tatcatctgg ccttcggggc gatggatcac
    22021 cgtttttccg cgccatctca tatcgtgctg gagaatgtca cgtttggtcg tgatggtcag
    22081 cccgcgcccc tggtggcaaa aagtgtcgac attgcgctaa gcagtcgcca actgaccgaa
    22141 ccacgccatg tcgataccat cctgctggaa aacgggacgc tgaatctcac cgaccagacc
    22201 gcgccgctac cgttcaaagc cgatcgtctg caactgcgtg atatggcgtt taatagcccg
    22261 aatagcgaat ggaaactgag cgcgcagcgg gtaaatggcg gcgtggttcc gtggtcacca
    22321 gaagccggta aagtgctggg tacgaaggcg cagattcagt ttagtgccgg atcgctttcg
    22381 ctcaatgatg ttcctgccac caatgtactg attgaaggca gtattgataa cgatcgcgtt
    22441 acgctgacta acctgggtgc cgacatcgcc cgcgggacat taaccggaaa cgcgcagcgt
    22501 aacgccgacg gcagctggca agtggaaaat ctgcgcatgg cggatatccg tctacaaagc
    22561 gaaaaatcgc taaccgactt ctttgcgcca ttacgctctg tcccgtcgtt gcagattggt
    22621 cgcttggaag tcatcgatgc tcgtctgcaa ggtccggact gggcggtgac cgacctcgat
    22681 ctcagcttgc gcaacatgac cttcagtaaa gatgactggc agacacagga aggcaaactg
    22741 tcgatgaacg ccagcgagtt catttatggt tcgctgcatt tatttgaccc gattataaac
    22801 gcggaatttt ccccgcaggg cgtagcgctg cgccagttca ccagccgctg ggaagggggt
    22861 atggtcagaa cgtcagggaa ctggctgcgt gacggtaaaa cgttgatcct tgatgatgcg
    22921 gcaattgccg ggctggaata taccttgccg aaaaactggc aacagttgtg gatggaaacg
    22981 acacccggtt ggttaaacag cctgcaactg aagagattta gcgccagccg caatctgatc
    23041 attgatatcg accctgactt cccgtggcag ctcaccgcgc tcgatggtta cggtgccaac
    23101 ctgacgctgg ttaccgatca taaatggggc gtctggagtg gctcggcgaa tctgaatgcc
    23161 gccgccgcga cattcaatcg tgttgatgtt cgtcgcccgt cgctggcgct gaccgccaac
    23221 agcagcacgg tgaatatcag cgaactgagt gcatttactg aaaaaggcat tctggaagcc
    23281 accgcaagtg tttcacaaac gccacaacgt cagacccata tcagcctgaa tggacgcggt
    23341 gtgccggtga atattttgca acaatgggga tggcctgaat taccgttgac tggcgacggc
    23401 aatattcagc ttaccgccag tggagatatt caggccaatg ccccgctgaa acctacggtt
    23461 agcgggcaat tgcatgccgt gaacgccgca aagcagcaag tgactcaaac catgaatgcg
    23521 ggcgtcgttt ccagcggtga agttacatcg acggagccgg tgcggtaaac atcaatctcg
    23581 ccgggtacga ctcagacgta cccggcattc cgtcaataga ttacaacgta attgtcagcg
    23641 cattcccttg aggcgtcacc accagcccct gctcactttc agcctgcgaa ccgccttgca
    23701 gaccatttac tttcacaaca ttgcgcaagc acagcgtcca gttcttcgcc tcgcccgcac
    23761 cagtcacagt aatcgtgttg ccagtacgtg ctgcttttaa agtaaagatc accgatccgt
    23821 cagcagcagg cacttcacag acggcttcat gcccgtcttg cagattaaag aggtggaatg
    23881 ccgtgccttc gtgccacacg taatcgggac gttgatcgtt gttgcccagc gccagtagag
    23941 tgttatcacg cacataaacg ggcagactca ggaagccgtg ctgctgttta tgccagcgac
    24001 taccgtcgag ttcatcgttg tgccacaggt gtgtccagcg accttccggc aggtagaact
    24061 gcacatcgcc cgcttcagtg aacaccggcg caaccatcac gttgtcgcct aacatgtatt
    24121 gacggtcaag gtaatcacaa gccggatcgt ccgggaactc catcatcatg gcccgcatca
    24181 tcggcgtacc ccgcgcgttc gcacgcgcag cttcacgata cagatacggc atcatgcggc
    24241 atttcagttg cgtgaagaag cgcaccacat cacaggactc atcatcgtag gcccacggca
    24301 cacgataaga tttgctaccg tgtaaacggc tatggctgga gagcaaacca aacgcgcacc
    24361 agcgtttgta aacgtgcgcc ggagcggtat tttcaaagcc gccgatatcg tggctccaga
    24421 agccaaaacc tgaaaggcca atagacaaac caccgcgcag gctttccgcc attgattcgt
    24481 agttagcgta acaatcgcca ccccagtgta ccgggaattt ctgcgcaccg acggaggccg
    24541 agcgggcaaa caagacagct tcttcctcac caacggtgtc cttgagcacg ttccacacca
    24601 gttcgttgta gatgtacgca taatggttat gcattttctg cgaatcggaa ccgtcaaacc
    24661 actgaacatc aattgggata cgttcgccaa agtcggtctt aaagcaatca acgcccatcg
    24721 cgaccagacc tttcagtttg tcggcgtacc atttgcaggc atccggattg gtaaagtcat
    24781 aaatcgccag acctggctgc catttatccc actgccatag cgaaccgtcc gggcgtttga
    24841 gtaaatagcc tttctcttgt aactctttaa agacggggga tttttgaccg atataggggt
    24901 taatccagac gcagattttc agccctttcg ctttcaggcg gcggatcatc ccttccgggt
    24961 cagggaaagt cagcgggtcc cactcaaaat cgcaccactg gaaggctttc atccagaaac
    25021 agtcaaagtg gaaaacatgc agcggcagat tgcgttccgc cataccatcg ataaagctgt
    25081 ttaccgtcgc ttcgtcgtag ttggtggtaa atgaagtggt tagccacagg ccgaaggacc
    25141 acgggggcgg cagcgccgga cgaccagtaa agcgggtata acgatcaagt accgctttcg
    25201 gcgtcgggcc gtcgataaca aagtattcga gatattcact ctcaacgctg aactgcactt
    25261 tggagacttt ctccgatccc acttcaaaag agacacactg gggatgattg accagtaccc
    25321 cataaccacg gttagtcatg tagaacggga tatttttata cgcctgttca gtacttgtgc
    25381 cgccgtcccg gttccaggtc tctaccgtct ggccattgcg caccagggca gtaaagcgct
    25441 ctcccagacc gtaaactgtt tcgccaacgc caagatcaag ccgctcaaac atataattgc
    25501 gttgattatt cgtgtcctgc acgtagccat tatttttcac ctgactaccg gtaatacgtt
    25561 cgccgttgcg cagaaaatcc agtgaccaga actcaccttt gctgacacgc gcgcttaagt
    25621 tgccactttt aaactcagca taacgttctg tgttttcgat tgtgaccttc acgtcctgca
    25681 aaatattgag cggataatga ggaccgttat tcagcgcccc ctgaaaatgc tcaatccgca
    25741 caccgacaat accttcctgt ggggagaaaa agcgcagcgt aaataaaggc gtatcaagct
    25801 gccaggtacg ttcacgcaca tcacgggggg cagcatagac caccatttca ttatcctgct
    25861 gttcaacctc gaatacctga agcgggtgaa tcaaattgag gccaggttga atcaaccagt
    25921 ttccatcgct aattttcatt gccgttcctt agttctgcac ttcttgagag gtgaattgtt
    25981 gctggcaacg gcgtttaccc tgagccagct gttccataac ggttttcaga ttgtgcgtcg
    26041 tgagtgagta gtagcgttta gcgataatcg cgctcagcaa ataacagatc gccggaacaa
    26101 tcgtgaatag cgcaataatg atgctaatcg tggcgctgtt ctgcgctttt tctgccgcat
    26161 catatccgcc ataagccagc atccagccga taagagcgcc gccgaaggcc aaccccagtt
    26221 tgagcacaaa cagcgtgcca gcaaaactga tcccggtcag gcgtttacca ttgcaccact
    26281 cgccgtagtc gacggtatcg aacatcatta cccactggat aggtgtcacc agttgatgca
    26341 acacaccaat cacgaagatg aagacaaaca tagtgatgct ggcctgcatg ggaacaaaga
    26401 acatcgcgag gctaatcact gccagcaggg cgttcgtcca ccagaagata gtgactttac
    26461 atttccagtc ggtcagaggt tttgccagtg cggaaccaat caggttaccc acgcaataag
    26521 tggtgagaaa agcgacaaac acttccggcg tgcccaaaat ccatgtgacg taatacatca
    26581 tcgccccacc gcgtacgcac accgccagga tattgaaaat ggttagtaaa ccgacaatcc
    26641 gccactggtc gttttgccag atatcacgta aatcttcccg catagacgtt gttgtaggtg
    26701 gtgcttcaac gcgttcttta gtggtgaaga aacaaaatgc cagcatcatg aatgccacca
    26761 cggaaaggac cacgataccg ccctggaaac cgagtggttt attatcaccg ccaattaaat
    26821 taaccagtgg catcatcaga acagtagaaa gcatgcctcc cgcggtcgcc agcacaaaac
    26881 gccaggattg cagcgagata cgctgagtcg ggtcattggt gattacacca cccaatgcgc
    26941 agtaagggat attgacgacg gtataaagta aggtaagtta gggtgtaagt aattgctgca
    27001 tagatcattt tgccgttcat actgagatct ggcgtgctat aggccagtac acagacgatc
    27061 ccgaatggca gtgcgccaaa cagtacccac ggacgaaatt taccccagcg agagcgcgtt
    27121 cgatcggcca acaaccccat gcaaggatcg gaaatcgcat ccagtgcacg agcgaccaaa
    27181 aacatggttc cgacaaatcc ggcaggaatg ccaaaaatat cggtataaaa gaacatcata
    27241 tataacatta cgttatcgaa aataatgtgg ctggcggcgt ctcccatgcc ataaccaatt
    27301 ttctctttaa cggacaacac ttcactcctc atctgctttt ccttgatgct ataggggtgc
    27361 tgagaccgat aacgcatttt gtaaaccatc cctgtgagag cgaatattcc gatatctggt
    27421 tattaattta tgattcttgt tttatgtgat cgtggtagcg ttaattccgc tcat
//
LOCUS       DAZPCS010000037        27383 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZPCS010000037 DAZPCS010000000
VERSION     DAZPCS010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27383
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.037"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..576
                     /locus_tag="ACHUQ7_001589"
     CDS             <1..576
                     /locus_tag="ACHUQ7_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1135056.1"
                     /translation="LAEEDPASATRPFRTEVKRSRLLIDEYLLIRKIADVQNEWFGLC
                     MDLALVTGQRKGDLAAMRWEDIRDSRLYVEQQKTGAKIRISLPTTISRLNLTLADVLD
                     NLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFHEIRSLSGRLHSAEK
                     GSDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            812..1630
                     /gene="ybhA"
                     /locus_tag="ACHUQ7_001590"
     CDS             812..1630
                     /gene="ybhA"
                     /locus_tag="ACHUQ7_001590"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HFR1135057.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETTQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIAHGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(1631..2689)
                     /gene="modC"
                     /locus_tag="ACHUQ7_001591"
     CDS             complement(1631..2689)
                     /gene="modC"
                     /locus_tag="ACHUQ7_001591"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFR1135058.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2692..3381)
                     /gene="modB"
                     /locus_tag="ACHUQ7_001592"
     CDS             complement(2692..3381)
                     /gene="modB"
                     /locus_tag="ACHUQ7_001592"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFR1135059.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3381..4154)
                     /gene="modA"
                     /locus_tag="ACHUQ7_001593"
     CDS             complement(3381..4154)
                     /gene="modA"
                     /locus_tag="ACHUQ7_001593"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR1135060.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4321..4470)
                     /gene="acrZ"
                     /locus_tag="ACHUQ7_001594"
     CDS             complement(4321..4470)
                     /gene="acrZ"
                     /locus_tag="ACHUQ7_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFR1135061.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4599..5387
                     /gene="modE"
                     /locus_tag="ACHUQ7_001595"
     CDS             4599..5387
                     /gene="modE"
                     /locus_tag="ACHUQ7_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFR1135062.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5455..6927
                     /gene="modF"
                     /locus_tag="ACHUQ7_001596"
     CDS             5455..6927
                     /gene="modF"
                     /locus_tag="ACHUQ7_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFR1135063.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDYTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDKIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQVVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            7188..8204
                     /gene="galE"
                     /locus_tag="ACHUQ7_001597"
     CDS             7188..8204
                     /gene="galE"
                     /locus_tag="ACHUQ7_001597"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFR1135064.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            8214..9260
                     /gene="galT"
                     /locus_tag="ACHUQ7_001598"
     CDS             8214..9260
                     /gene="galT"
                     /locus_tag="ACHUQ7_001598"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFR1135065.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAECEDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            9264..10412
                     /gene="galK"
                     /locus_tag="ACHUQ7_001599"
     CDS             9264..10412
                     /gene="galK"
                     /locus_tag="ACHUQ7_001599"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFR1135066.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            10406..11446
                     /gene="galM"
                     /locus_tag="ACHUQ7_001600"
     CDS             10406..11446
                     /gene="galM"
                     /locus_tag="ACHUQ7_001600"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFR1135067.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            11649..12401
                     /gene="gpmA"
                     /locus_tag="ACHUQ7_001601"
     CDS             11649..12401
                     /gene="gpmA"
                     /locus_tag="ACHUQ7_001601"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFR1135068.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12568..13620)
                     /gene="aroG"
                     /locus_tag="ACHUQ7_001602"
     CDS             complement(12568..13620)
                     /gene="aroG"
                     /locus_tag="ACHUQ7_001602"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFR1135069.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLVNAVKARRG"
     gene            13936..14316
                     /locus_tag="ACHUQ7_001603"
     CDS             13936..14316
                     /locus_tag="ACHUQ7_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFR1135070.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14430..15371
                     /gene="zitB"
                     /locus_tag="ACHUQ7_001604"
     CDS             14430..15371
                     /gene="zitB"
                     /locus_tag="ACHUQ7_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFR1135071.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHTTIQMEYQPCHRPDCHLNEGVSGHSHHHH"
     gene            complement(15368..16087)
                     /gene="pnuC"
                     /locus_tag="ACHUQ7_001605"
     CDS             complement(15368..16087)
                     /gene="pnuC"
                     /locus_tag="ACHUQ7_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFR1135072.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(16125..17168)
                     /gene="nadA"
                     /locus_tag="ACHUQ7_001606"
     CDS             complement(16125..17168)
                     /gene="nadA"
                     /locus_tag="ACHUQ7_001606"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFR1135073.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQVECSLDLGCPVEEFNAFYDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNDLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(17417..17492)
                     /locus_tag="ACHUQ7_001607"
     tRNA            complement(17417..17492)
                     /locus_tag="ACHUQ7_001607"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17457..17459),aa:Lys,seq:ttt)
     gene            complement(17496..17571)
                     /locus_tag="ACHUQ7_001608"
     tRNA            complement(17496..17571)
                     /locus_tag="ACHUQ7_001608"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17536..17538),aa:Val,seq:tac)
     gene            complement(17623..17698)
                     /locus_tag="ACHUQ7_001609"
     tRNA            complement(17623..17698)
                     /locus_tag="ACHUQ7_001609"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17663..17665),aa:Lys,seq:ttt)
     gene            complement(17701..17776)
                     /locus_tag="ACHUQ7_001610"
     tRNA            complement(17701..17776)
                     /locus_tag="ACHUQ7_001610"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17741..17743),aa:Val,seq:tac)
     gene            complement(17912..17987)
                     /locus_tag="ACHUQ7_001611"
     tRNA            complement(17912..17987)
                     /locus_tag="ACHUQ7_001611"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17952..17954),aa:Lys,seq:ttt)
     gene            complement(18152..18943)
                     /gene="cpoB"
                     /locus_tag="ACHUQ7_001612"
     CDS             complement(18152..18943)
                     /gene="cpoB"
                     /locus_tag="ACHUQ7_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFR1135074.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVSSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGATASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(18953..19474)
                     /gene="pal"
                     /locus_tag="ACHUQ7_001613"
     CDS             complement(18953..19474)
                     /gene="pal"
                     /locus_tag="ACHUQ7_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFR1135075.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(19509..20801)
                     /gene="tolB"
                     /locus_tag="ACHUQ7_001614"
     CDS             complement(19509..20801)
                     /gene="tolB"
                     /locus_tag="ACHUQ7_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFR1135076.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(20934..22175)
                     /gene="tolA"
                     /locus_tag="ACHUQ7_001615"
     CDS             complement(20934..22175)
                     /gene="tolA"
                     /locus_tag="ACHUQ7_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFR1135077.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEK
                     AAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAG
                     QIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKI
                     PKPPSQAVYEVFKNAPLDFKP"
     gene            complement(22240..22668)
                     /gene="tolR"
                     /locus_tag="ACHUQ7_001616"
     CDS             complement(22240..22668)
                     /gene="tolR"
                     /locus_tag="ACHUQ7_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFR1135078.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(22672..23364)
                     /gene="tolQ"
                     /locus_tag="ACHUQ7_001617"
     CDS             complement(22672..23364)
                     /gene="tolQ"
                     /locus_tag="ACHUQ7_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFR1135079.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(23361..23765)
                     /gene="ybgC"
                     /locus_tag="ACHUQ7_001618"
     CDS             complement(23361..23765)
                     /gene="ybgC"
                     /locus_tag="ACHUQ7_001618"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFR1135080.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(23915..24208)
                     /gene="ybgE"
                     /locus_tag="ACHUQ7_001619"
     CDS             complement(23915..24208)
                     /gene="ybgE"
                     /locus_tag="ACHUQ7_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFR1135081.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(24208..24321)
                     /gene="cydX"
                     /locus_tag="ACHUQ7_001620"
     CDS             complement(24208..24321)
                     /gene="cydX"
                     /locus_tag="ACHUQ7_001620"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFR1135082.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(24336..25475)
                     /gene="cydB"
                     /locus_tag="ACHUQ7_001621"
     CDS             complement(24336..25475)
                     /gene="cydB"
                     /locus_tag="ACHUQ7_001621"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFR1135083.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLL
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(25491..27059)
                     /gene="cydA"
                     /locus_tag="ACHUQ7_001622"
     CDS             complement(25491..27059)
                     /gene="cydA"
                     /locus_tag="ACHUQ7_001622"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFR1135084.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQTVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT         6892 a         7260 c         6704 g         6527 t
ORIGIN      
        1 cttgccgaag aagatccagc ttctgcaacc aggccattca gaactgaagt gaagcgcagt
       61 cgactgttaa ttgacgagta tctgttaatc aggaaaatag cagacgtcca gaacgaatgg
      121 ttcggccttt gcatggatct tgcactggta acaggacagc gcaaaggaga tctggcggct
      181 atgagatggg aggatataag agatagcagg ctatacgtgg aacaacaaaa aacaggggca
      241 aaaattcgca tttctctccc caccacgatt tccagattga atttaacact tgccgatgta
      301 ctggataact tgaaaaaaat aaacggtaaa aacgaaaagt tactcggagg gaagacagcc
      361 aggacaattg cagcacagtt caggattgcc cgagacacat ccggattgaa atgggaaggt
      421 gacccaccgc catttcatga aatccgaagt ttatccggtc gcctgcactc agcagaaaaa
      481 ggcagtgatt tcactcaggc gctccttgga catcgttcat ccagcatgac ggataaatac
      541 cgtgacggac gagggaggga atggaaagac atttagaatt aatttcgaat gatttcgaaa
      601 tgccacttgt aattaattga ttataaagaa ttaattccta aagagcattt gcttcatgaa
      661 tgctcctttg catttagcaa ctgactgtaa cagctaaaat tagtcgcttt tggcggtgaa
      721 tgctgaatta aaacgcagaa ttttgtccga tagtggtatc ggtgtaccat tcgcgcagac
      781 gaaaattctt aaccttaatc tggaatacgc catgaccaca cgcgtgattg ctctcgactt
      841 agacggcacc ttattgaccc cgaaaaagac cttgcttcca tcatcgatag aagccctggc
      901 ccgcgctcgc gaagcaggct atcaattaat catcgtcaca ggtcgccatc acgtcgctat
      961 tcatcctttt tatcaggcgc tggcgctgga tacacctgct atttgctgta atggcaccta
     1021 tttgtatgat tatcatgcaa aaaccgtgct ggaagcggac ccaatgcccg ttaataaagc
     1081 cctgcaactc attgagatgc tgaatgaaca ccacattcac ggtctgatgt atgtcgatga
     1141 tgcaatggtc tatgagcacc cgaccgggca tgtcattcgc acgtctaact gggcgcaaac
     1201 cctgccgccg gaacagcgtc cgactttcac acaagtcgct tctctggctg aaacgacgca
     1261 acaagttaac gccgtatgga agttcgccct gacgcacgat gacctgccgc aattgcagca
     1321 ttttggtaag catgtcgaac atgaactggg actggagtgt gaatggtcct ggcacgatca
     1381 ggttgatatt gcacacggcg gcaacagcaa aggtaaacgt ttgacgaaat gggttgaggc
     1441 gcaaggttgg tcgatggaaa acgtcgtggc attcggcgat aactttaatg atatcagtat
     1501 gctggaagcc gctggtacag gcgtggcgat gggcaacgcc gatgacgcgg taaaagcgcg
     1561 cgccaacatt gtgattggtg ataacaccac cgacagcatt gcccagttca tttatagcca
     1621 cctgatttaa tcaggcggtt atcgacacac ttttaatttg cgcgtacagc cacaggccag
     1681 gtttgatcgc cagttcatcc ctggcccacg ggctgatacg cgcccacagc gttttaccgc
     1741 cgacttccag ctccacttcc acctggccgt tgtcgtcata actattaaca acttttgccc
     1801 gcaatacgtt acgaatgctg gtttgctgcg gcggttgtaa aaccagagaa acatcggaag
     1861 cctgaatgcg gatacgtagc gcagcttgca gcggttcgcc cagcttattg acccacaaat
     1921 gctgatcgcc cagcgccagc gcggtcatcg cgtaatgcgg atgatgctca agcaccgtca
     1981 ctttcagaat gctactttgt tgctctttcg gcagccacgg attcatcacg ctactgcccc
     2041 acacttcctc cagcgcgcca aaggctttca cctgaccgtt ttccagtacc atcactctgt
     2101 ctgccagatg gaggatctca tccagcgaat ggctgacata caacatcgga atgttgattt
     2161 cccgtgtcag ccgttgcaga taaggcaaca gttcgcgttt acgcggaata tccagtgacg
     2221 ccagcggttc atccagcaac agcaattccg gtgctgtcag caaagcccga ccaatcgcca
     2281 cgcgctgttt ttcgcctccg gacaggctgc ctggtaaacg gtcaagcaac ggttcaatgc
     2341 ctaaaagcgc caccagctta tcgaactgat cgaccatact tttcgacatg ccgtagcgca
     2401 gattgccacg cactttgtaa tgcgggaaca accgcgcatc ctgaaaaaca tagccaacac
     2461 gacgcttttc cggcgtcagg cagatacctt tttcggcatc atttagtacc cgcccattga
     2521 ggacaatccg ccctttttgc gggcgcgtca gtccactgat ggcgttaatc agcgaagttt
     2581 ttccggcacc ggagacgcca aagatagcag tgatgccatt ggcgggcagc gtttcattaa
     2641 tggtcaggca atggttgccc aacgtctggg aaaaattcag ctccagcatg attagcgccc
     2701 cgcccgttca cggctgattc tggccagcca ttctgaaatc aacagggaga tcatcgccag
     2761 cgcaatagaa ataatgcaca gtctcgccgc gccactttcc ccgccggggg tctggatcag
     2821 ggtatacatg gcagaaggaa tggttcgcgt ttcgctagga atgttcgaca caaaggtgat
     2881 ggttgcacca aactcaccga gagaacgagc aaaagccagt accgtaccaa caataattcc
     2941 cggtaaggtc agcggtaacg tgatagtaaa gaaaacgcgc cagcgcccgg cacctagtgt
     3001 tcttgcggcc tgttccagtt tgacatcaac cccttccagc gccagacgaa ttgcccgcac
     3061 catcagcgga aacgacatga cggcggcagc gagaaccgcg ccgcgccagc taaaggcgaa
     3121 ggtaatacca aaccagtcat acagacgttc accgataaat ccgcgccgtc ccatcgaaac
     3181 taataataag taaccgacga ccacgggcgg taacaccagc ggtagatgca gtacgctgtc
     3241 gagcagagct ttgcccggaa acgtgcaacg caccagtaac caggcaaaaa agatcccaaa
     3301 cggcaggcta aacagcacag ccagggaaga aactttcagg cttaataaaa ctgcctgcca
     3361 ttctggatcg gtcagtatca ttacttggtt gtaaatccgt aacgtttaaa gatttccgct
     3421 gcctgcggtc ccttcagata atcataaaat gctttcactg tcgcattgtt atgcccttcc
     3481 acaacagcaa ccggatattc cactttttta tgtgaatctt ccgggaaggt ggcaaccact
     3541 tttacccctt tgctggcaac tgcgtcagaa ccgtagacaa tgcccagagg cgcttcgtta
     3601 cgttcgacca gcgccagcgc cccacgaaca tcttccgctg gggccagttt cggagagagc
     3661 gtatcccatg cgcccagttt ttgcagtgct tcttttgcat aaatgccagc gggaacatgt
     3721 tccggatcgc caaccgccag gcgaccgcca ttcagcagcg aagtccagtt agttttgctg
     3781 tcgatggtga aatctttctg ctcgctggct ttcggtgcta caacgaccag gctattgccg
     3841 agcagtgtct gacgcgtagc agtatcgatc gcttttttat caaccgcata atccatccat
     3901 ttctgatcgg cagaaataaa cagatccgca ggggcacccg cttcaatctg acgggcgaga
     3961 gtagatgacg aagcgaaaga agaaaccaca tccacgcctt tctctttttt atactgcgta
     4021 gcaatgtcct gcattgcgtt agtcagtgat gcggcggcga acaccgtgat tttcccttca
     4081 tctgccagtg cattgccagc aactgcgaaa gagagtgccg ccccggcaaa caagttcaac
     4141 catttacgag ccattggtaa ccccttaatg taacgttatg taggcgacaa tataacgact
     4201 aactcggaat tttcccagca gttattgcta acctttgtga ggtagataag aaaaaatatc
     4261 ggcaggaaaa gcaggaagtt gagaaaaaga aaatgcccga ctaagcgggc actcagggaa
     4321 tcaatgattt tgtccgggct ggtctttttt accaacacca gaaaagatgt tgaatacttc
     4381 accaagaccg taaatcagac ccaggatgat ggccatcacg acaggtacca tgattacggc
     4441 gaataccaga ctttttaata actctaacat ggtcaactcc agatatagtc atgagactat
     4501 tctaaccgct aagcacagaa aagcactccc cttttgtgcg gtcagctttg cgtggcgttc
     4561 gttttccgtc acaataagac ttttgccagg acattgttat gcaggccgaa atccttctca
     4621 cccttaagct ccaacaaaaa ttattcgccg acccgcgccg catttcgcta ctaaaacaca
     4681 ttgcgctttc cggttccatt agccagggag cgaaagatgc cggtatcagc tataaaagcg
     4741 cctgggatgc cattaacgag atgaatcagt taagtgagca tattctggtc gagcgcgcaa
     4801 caggcggtaa aggtggcgga ggcgcagtac tgacccgcta tggtcagcga ctgattcagc
     4861 tctatgactt actggcgcaa atccagcaaa aagcctttga tgtgttaagt gacgatgacg
     4921 ccctgccgct gaacagcctg ctggccgcga tctcacgttt ttcactgcaa accagcgccc
     4981 gtaaccagtg gttcggtacc atcaccgccc gcgatcatga tgacgttcaa cagcatgttg
     5041 atgtcttact ggctgacgga aaaacacgcc tgaaagtcgc aattaccgca caaagcggcg
     5101 cacgtctggg gctggatgaa ggcaaagaag tgttgatatt gctaaaagcg ccgtgggtag
     5161 gcattactca ggacgaagcg gtcgcacaaa acgccgacaa ccaattaccg ggtattatta
     5221 gtcatattga gcgcggcgca gagcagtgcg aagtattaat ggcgctaccc gacgggcaaa
     5281 cactgtgcgc cacagtgccg gtaaatgaag cgacttctct tcagcaaggg cagaatgtca
     5341 cggcctactt taatgccgac agcgtgatta tcgccacgct gtgctaagcg tgttgacaat
     5401 ttgttatgaa acacgtatcc ctgtcagtaa tcgctgcaca aaatgggata taaaatgtca
     5461 tcgttgcaaa ttttgcaagg cacgtttcgt cttagcgaca caaaaacgct gcaattgcct
     5521 cagctaacgt taaacgcggg tgatagttgg gcgtttgtcg gttcgaatgg aagtggaaag
     5581 tcggcgctgg cccgcgcgct ggcgggggaa cttccgcttt tgaaaggtga acggcaaagc
     5641 cagttttccc acatcactcg tctctccttc gagcaattgc aaaagctcgt cagcgacgaa
     5701 tggcaacgga ataacaccga tatgctcggc cctagcgaag attacaccgg acgcactacg
     5761 gctgagatta ttcaggatga agtaaaggat gcaccgcgtt gcatgcaact ggcgcagcag
     5821 ttcggtatta ccgccctcct cgaccgacgc tttaaatacc tttccactgg cgagacgcga
     5881 aaaaccctgc tgtgtcaggc gctgatgtcg gagcctgact tgttgattct tgatgagccg
     5941 ttcgatggcc tggatgttgc ctcacgtcag cagctggctg agcgactcgc ctcgttacat
     6001 cagtccggta ttactttggt actggtgctc aatcgcttcg ataagatccc ggaatttgtc
     6061 cagtttgctg gcgtgctggc ggattgtacg ttagcggaaa ctggcgctaa agaggaactg
     6121 ctccagcaag cactcgtcgc gcaacttgcg catagtgaac agcttgaagg tgtgcaactg
     6181 ccggagccgg atgaaccttc agcacgtcac gccttacccg ccaacgaatc gcgcattgtg
     6241 ctgaacaatg gcgtggtttc ttataatgat cgccccattc ttaataacct tagctggcag
     6301 gtgaatccag gcgaacactg gcaaattgtc gggccaaatg gcgcgggaaa atcgacgtta
     6361 ttaagcctga ttactggcga tcatccgcaa ggttacagca acgatttgac gcttttcggc
     6421 cgacgtcgcg gcagcagcga aaccatctgg gacatcaaaa agcatatcgg ttacgtcagc
     6481 agtagtttgc atctggatta ccgggtcagc actaccgtgc gtaatgtgat tctttctggc
     6541 tattttgatt cgattggcat ttatcaggtc gtttcggatc gccagcaaaa actggtgcag
     6601 cagtggctgg atattctcgg cattgataaa cacacggctg acgctccgtt ccatagtctt
     6661 tcctggggac agcagcgttt ggcgctgatc gtccgcgcac tggtgaaaca tccgacgttg
     6721 cttattctcg atgaaccact acaggggctt gatccgctca atcgccaact tatccgccgt
     6781 tttgttgatg tgctgattag cgaaggtgaa acgcaattgt tgtttgtttc gcaccacgct
     6841 gaagatgcgc ctgcctgtat tacccatcgc cttgagttcg tgccggacgg tggactctat
     6901 cgctatgtgc tgacaaaaat atattgagtc ggtagtgctg accttgccgg aggcggccta
     6961 agcgcactct ccggccaacg gttcgacgca tgcaggcatg aaaccgcttc ttttttcaga
     7021 taaaaagcgc aatcattcat aaaccctctg ttttataatc acttaatcgc gcataaaaaa
     7081 cggctaaatt cttgtgtaaa cgattccact aatttattcc atgtcacact tttcgcatct
     7141 ttgttatgct atggttattt cataccataa gcctaatgga gcgaattatg agagttctgg
     7201 ttaccggtgg tagcggttac attggaagtc atacctgcgt gcaattactg caaaacggtc
     7261 atgatgtcat cattcttgat aacctctgta acagtaagcg cagcgtactg cctgttatcg
     7321 agcgtttagg cggcaaacat ccaacgtttg ttgaaggcga tattcgtaac gaagcgttga
     7381 tgaccgagat cctgcacgat cacgctatcg acaccgtgat ccacttcgcc gggctgaaag
     7441 ccgtcgccga atcggtacaa aaaccgctgg aatattacga caacaatgtc aacggcactc
     7501 tgcgcctgat tagcgccatg cgcgccgcta acgtcaaaaa ctttattttt agctcctccg
     7561 ccaccgttta tggcgatcag ccccaaattc catacgttga aagcttcccg accggcacac
     7621 cgcaaagccc ttatggcaaa agcaaattga tggtggaaca gatcctcacc gacctgcaaa
     7681 aagcccagcc ggactggagc attgccctgc tgcgctactt caacccggtt ggcgcgcatc
     7741 cgtcgggcga tatgggcgaa gatccgcaag gcattccgaa taacctgatg ccatacatcg
     7801 cccaagttgc tgtaggccgt cgcgactcgc tggcgatttt tggtaacgat tatccgaccg
     7861 aagatggtac tggcgtacgc gattacatcc acgtaatgga tctggcggac ggtcacgtcg
     7921 tggcgatgga aaaactggcg aacaagccag gcgtacacat ctacaacctc ggtgctggcg
     7981 taggcagcag cgtgctggac gtggttaatg ccttcagcaa agcctgcggc aaaccggtta
     8041 actatcattt tgcaccgcgt cgcgaaggcg accttccggc ctactgggcg gacgccagca
     8101 aagccgaccg tgaactgaac tggcgcgtaa cgcgcacact cgatgaaatg gcgcaggaca
     8161 cctggcactg gcagtcacgc catccacagg gatatcccga ttaaggaacg accatgacgc
     8221 aatttaatcc cgttgatcat ccacatcgcc gctacaaccc gctcaccggg caatggattc
     8281 tggtttcacc gcaccgcgct aagcgcccct ggcagggggc gcaggaaacg ccagccaaac
     8341 aggtgttacc tgcgcacgat ccagattgct tcctctgcgc aggtaatgtg cgggtgacag
     8401 gcgataaaaa ccccgattac accgggactt acgttttcac taatgacttt gcggccttga
     8461 tgtctgacac gccagatgcg ccagaaagca acgatccttt gatgcgttgc cagagcgcgc
     8521 gcggtaccag ccgggtgatc tgcttttcac cggatcacag taaaacgttg ccagaactga
     8581 gcgttgcggc attgacggaa atcgtcaaaa cctggcagga gcaaaccgca gagctgggaa
     8641 aaacgtaccc gtgggtgcag gtctttgaaa acaaaggtgc ggcgatgggc tgctctaacc
     8701 cgcatccgca cggacagatt tgggcaaata gcttcctgcc taacgaagct gagtgcgaag
     8761 accgcctgca aaaagaatat tttgccgaac agaaatcacc aatgctggtg gattatgttc
     8821 agcgcgagct ggcagacggt agccgtaccg ttgtcgaaac cgaacactgg ttagccgttg
     8881 tgccttactg ggctgcctgg ccgttcgaaa cgctactgct gcccaaagcc cacgttttgc
     8941 ggatcaccga tttgaccgac gcccagcgca gcgatttggc gctggcgttg aaaaagctga
     9001 ccagtcgtta tgacaacctc ttccagtgct ccttccccta ctctatgggc tggcacggcg
     9061 caccatttaa tggcgaagag aatcaacact ggcagctgca cgcgcacttt tatccgcctc
     9121 tgctgcgctc cgccaccgta cgtaaattta tggttggtta tgaaatgctg gcagagaccc
     9181 agcgagacct gaccgcagaa caggcagcag agcgtttgcg cgcagtcagc gatatccatt
     9241 ttcgcgaatc cggagtgtaa gaaatgagtc tgaaagaaaa aacacaatct ctgtttgcca
     9301 acgcatttgg ctaccctgcc actcacacca ttcaggcgcc tggccgcgtg aatttgattg
     9361 gtgaacacac cgactacaac gacggtttcg ttctgccctg cgcgattgat tatcaaaccg
     9421 tgatcagctg tgcaccacgc gatgaccgta aagttcgcgt gatggcagcc gattatgaaa
     9481 atcagctcga cgagttttcc ctcaatgcgc ccattgtcgc gcatgaaaac tatcaatggg
     9541 cgaactacgt tcgtggcgtg gtgaaacatc tgcaactgcg taacaacagc ttcggcggtg
     9601 tggacatggt gatcagcggc aatgtgccgc agggtgccgg gttaagttct tccgcttcac
     9661 tggaagtcgc ggtcggaacc gtattgcagc agctttatca tctgccgctg gacggcgcac
     9721 aaatcgcgct taacggtcag gaagcagaaa accagtttgt tggctgtaac tgcgggatca
     9781 tggatcagct aatttccgca ctcggcaaga aagatcatgc cttgctgatt gactgccgct
     9841 cactggggac taaagcagtt tccatgccga aaggtgtggc tgtcgtcatc atcaacagta
     9901 acttcaaacg taccctggtt ggcagcgaat acaacacccg tcgtgaacag tgcgaaaccg
     9961 gtgcgcgttt cttccagcag ccagctctgc gcgatgtcac cattgaagag ttcaatgctg
    10021 ttgcacatga gctggaccca atcgtggcga aacgcgtgcg tcatatcctg actgaaaacg
    10081 cccgcaccgt tgaagctgcc agcgcgctgg agcaaggcga cctgaaacgt atgggcgagt
    10141 tgatggccga gtctcatgcc tctatgcgcg atgatttcga aatcaccgtg ccgcaaattg
    10201 acactctggt agaaatcgtc aaagctgtga ttggcgacaa aggtggcgta cgcatgaccg
    10261 gcggcggatt tggcggctgt atcgtcgcgc tgatcccgga agagctggtg cctgccgtac
    10321 agcaagctgt cgctgaacaa tatgaagcaa aaacaggtat taaagagact ttttacgttt
    10381 gtaaaccatc acaaggagca ggacagtgct gaacgaaact cccgcactgg cacccgatgg
    10441 tcagccgtac cgactgttaa ctttgcgtaa caacgcaggg atggtagtca cgctgatgga
    10501 ctggggtgcg actttacttt ccgcccgtat tccgctttcc gatggcagcg tccgcgaggc
    10561 gctgctcggc tgtgccagtc cggaatgcta tcaggatcag gctgcgtttc tgggggcctc
    10621 tattggtcgt tatgccaacc gcatcgccaa tagtcgctat acctttgacg gtgaaaccgt
    10681 gacgctttcg ccaagccagg gcgttaacca gctgcacggc ggcccggaag ggttcgacaa
    10741 acgtcgctgg cagattgtga accagaacga tcgtcaggtg ctgtttgccc tgagttcaga
    10801 tgatggcgat cagggcttcc cgggtaatct cggcgcgacg gtgcaatatc gtctgaccga
    10861 cgataaccgt atctccatta cttatcgcgc cacagttgat aaaccttgcc cggtgaatat
    10921 gactaatcac gtctatttca atcttgacgg cgagcagtct gacgtgcgca atcacaagtt
    10981 gcagattctg gcggacgaat atcttccggt tgatgaaggc ggcattccgc acgacggctt
    11041 gaaatctgtc gccggaacgt cttttgattt ccgcagcgcc aaaatcatcg ccagtgagtt
    11101 tcttgccgac gacgatcagc gcaaagtgaa aggttacgat cacgcattct tgttacaggc
    11161 caaaggcgat ggcaagaaag tggcggcgca tgtctggtca gcagatgaaa aattgcagct
    11221 gaaggtctac accaccgctc cggctcttca attctactcc ggcaacttcc tcggcggcac
    11281 accgtcgcgg ggaaccgaac cttacgccga ctggcaaggg ctggcgttgg aaagcgagtt
    11341 tctgccggac agcccgaacc accctgaatg gccgcaaccg gactgcttcc tgcgtcctgg
    11401 cgaagagtat tccagcctga cggaatatca gtttattgct gagtaatgtt aagccctcgt
    11461 catgagggct ttatcttcat attgttcaaa tcaccagcaa acaccgacat atttgcaact
    11521 caatattcac aacaacctta cactgcgcca ctattttcgc tatggttatg cgtaagcatt
    11581 gctgttgctt cgtcacggca atataatgag aattattatc attaaaagat gatttgagga
    11641 gtaagcatat ggctgtaact aagctggttc tggttcgtca tggcgaaagt cagtggaaca
    11701 aagaaaaccg tttcaccggt tggtacgacg tggatctgtc tgagaaaggc gtaagcgaag
    11761 caaaagcagc aggtaagctg ctgaaagagg aaggttacag ctttgacttt gcttacactt
    11821 ctgtgctgaa acgcgctatc cataccctgt ggaatgtgct ggacgaactg gatcaggcat
    11881 ggctgcccgt tgagaaatcc tggaaactga acgaacgtca ctacggtgcg ttgcagggtc
    11941 tgaacaaagc ggaaactgct gaaaagtatg gcgacgagca ggtgaaacag tggcgtcgtg
    12001 gttttgcagt gactccgccg gaactgacta aagatgatga gcgttatccg ggtcacgatc
    12061 cgcgttacgc gaaactgagc gagaaagaat tgccgctgac ggaaagcctg gcgctgacca
    12121 ttgaccgcgt gatcccttac tggaatgaaa ccattctgcc gcgtatgaag agcggtgagc
    12181 gcgtaatcat cgccgcacac ggtaactccc tgcgtgcgct ggtgaaatat cttgataaca
    12241 tgagcgaaga agagattctt gagctgaata tcccgactgg cgtgccgctg gtgtatgagt
    12301 tcgacgagaa tttcaaaccg ctgaaacgct attatctggg taatgctgac gagatcgcgg
    12361 cgaaagcagc agccgttgcg aaccagggta aagcgaagta aacgtcattc gtttaaaaaa
    12421 tgagaaagcc gactgcaagt gagtcggctt ttttgtttgt cgttgctaac aatggagcac
    12481 attgcctgat gcgacgctgc gcgtcttatc aggcctgtgg tgattcatcg gatacgccac
    12541 tctgacggcg catccgacaa ttaaacctta cccgcgacgc gcttttactg cattcaccag
    12601 ttgacgtaac agagcatcgg tatcttccca gccaatgcag gcatcggtga tgctcttacc
    12661 gtaggccagc ggctccccgc tatcgaggct ctgattgcct tccaccagat ggctttccac
    12721 catcacgcca ataatggcct tttcgccacc ggcaatctgc tggcaaacgt cagcacaaac
    12781 atccatctgc tttttgaatt gtttggacga attagcatgg ctgaaatcga tcatcacctg
    12841 agctggcaga cctgctttgt tcagcccttc tttcacttca gcaacgtgct tcgcgctgta
    12901 gttaggctct ttaccgccgc gcagaatgat atggcaatcg ccgttaccac tggtattcac
    12961 aatcgccgaa tgcccccatt tagttacgga caggaagcag tgcggcgcac cggcggcatt
    13021 aatggcatcg atagccactt taatcgtacc gtctgtgcca tttttaaaac ctaccggaca
    13081 agaaagacca gacgccagtt cgcggtgcac ctgcgattcc gtggtacgtg caccaattgc
    13141 gccccagctc atcaggtcag cgagatactg aggagtgatc atatcgagaa actcacccgc
    13201 cgctggcaga ccgctgtcgt taatatcaag cagcaattta cgggctatac gcagaccgtc
    13261 gttgatctgg aagctgttat ccatatgcgg atcgttaatc aaccctttcc agcccaccgt
    13321 ggtacgcggc ttttcaaaat agacgcgcat tacgatttcc agctcatctt tcagctcttc
    13381 acgcagcgcc agcaagcgag tggcatactc tttagccgcg acaggatcat gaattgagca
    13441 cgggccaatc accaccaaca ggcgatcatc attacctttc aggatcttat ggatcgcttt
    13501 tcgggcatgg gcaaccgtat tcgcggcatt ttcagttgcg gggaattttt ccagcaatgc
    13561 gacaggagga agtaactctt tgatttcttt gatgcgtaaa tcgtcgttct gataattcat
    13621 gtctgttcca gtgttgccat acttatctta gtgaatgcaa tacttccaat ctatatcttc
    13681 cgtcagaatg tgtaaacggg gttttacact atgaacggat taatcctgga attagcaaga
    13741 aaaacgccag attgtcgcga aaaacgagat ctctcctaca atttctaact gtaactcctt
    13801 tgtttgttaa ttatttcaag attctctgct gcgtttcata aactggctga aatcttaaac
    13861 caatgcctta tattcacctg caaatgcact gttggaagag gttatccgac ataacgacca
    13921 taacaggagc atcctatgaa aatgacaaaa ctggccacat tatttctgac tgccactcta
    13981 agccttgcca gcggtgccgc actggctgcc gatagcggag cgcaaactaa taacggccag
    14041 gcaaacgccg cagctgatgc gggccaggta gcccctgacg cccgtgaaaa tgtcgcgcca
    14101 aataacgtcg acaataacgg ggtaaatacc ggttctggcg gcacaatgct gcattcggat
    14161 ggttcttcaa tgaacaatga cggaatgacc aaagatgaag agcacaagaa caccatgtgc
    14221 aaagatggtc gatgtccgga tatcaataaa aaggtacaaa ccggtgatgg catcaataat
    14281 gatgtcgata ccaaaaccga cggtaccacg cagtaaccct gcaaaatagc caataatggg
    14341 agagcggagt ctctcccttt taatttttca cttctaaaag cagtatgatg tcataaagtt
    14401 aagtgagaat aattaaggga tgactcgtca tggcgcactc acactcacac acgtcctcac
    14461 acctgccaga agataataat gctcgtcgct tgttgtatgc tttcggcgtg actgccgggt
    14521 ttatgctggt agaagtcgtt ggtggttttc tttctggttc tctggcattg ctggccgatg
    14581 cgggtcatat gttgaccgat actgccgccc tgcttttcgc cctactcgcc gtgcaatttt
    14641 cacgtcgtcc tcccaccatt cgccacactt tcggttggct aagactgacc acgctcgcgg
    14701 cgtttgtgaa tgccatcgcc ctcgtggtaa ttaccatttt gattgtctgg gaggcgatag
    14761 aacgcttccg cacgccgcgt ccggtcgagg gaggcatgat gatggcaatt gccgtggctg
    14821 ggttgctggc aaatatactt tctttctggt tacttcatca cggcagcgaa gagaaaaacc
    14881 tcaacgtacg agcagcggca ctgcatgtgc tcggggacct gctgggttcg gttggggcta
    14941 ttattgccgc gttgattatt atctggactg gctggacacc tgctgacccc attctctcga
    15001 tactggtgtc gcttctggtt ctgcgtagcg cgtggcgact gttgaaagat agcgtgaatg
    15061 aattacttga aggtgcaccg gtatcgctgg atatcgctga actgaagcgt cgtatgtgcc
    15121 gggaaatccc ggaagtccgc aatgtgcacc atgtacatgt atggatggta ggcgagaagc
    15181 cggtgatgac gttgcatgtg caggtgatcc cgccacacga tcacgatgcc ttgttggatc
    15241 agatccaaca ctacctgatg gatcactatc agattgagca caccaccatt cagatggaat
    15301 atcaaccttg tcataggcca gactgccacc tgaatgaagg ggtgtctggc cattcacatc
    15361 atcaccatta atgggacagc gcgcgtgagc ctctttcacg tgcgctgttg atccacatcc
    15421 ggctgccgtt gagcgcaata aaggtcagga tgatgtactc cagagacatg gcgtaaacgc
    15481 cctgaagtgc aaaaataacg acgctaatca cgttaataat cacccacaac agccagtttt
    15541 ccacatactt acgcgtcatc agaatcattg ccacgataga taacaccatc atgcatgaat
    15601 cccagaacgg gaaagcgtcc ggttgcagtt caggcatcac aacctgtaat cctaatgctt
    15661 gcatgatcat gactgccacg cgggtcaaaa atgcaaacac cggattgata aataccgtca
    15721 tcagaccaat cgaaacaacg caaaccgcca accagctgag tgccttcggc aatggcaacc
    15781 agcgaatttt caactccgcc tcgttctgac tggtttgtcg cgaccacgca taccagccgt
    15841 aaatattcgc ggcaaagaaa aacacctgta atagcaggct ggcatacagc tgaatctgaa
    15901 agaaaataat gccaaacaag gtaacgttga tcaggccaaa gaagtagttg ctgatcttct
    15961 ccagactggc aaggccaata cacagcaacc cggcgatcgt gcctaccgct tcgatccatg
    16021 agagatcata accgcccgcc cctattggta tatgtaccag gatattctgc acactaaaaa
    16081 aatccatctt tcccccaaag cgtaacgtta cgccttatta ttcgttatcc acgtagtgta
    16141 gccgcaaaat ccagcatacg attgagcggc accagcgccc tctctcgcag cctctcatcg
    16201 acatgaacct cgtgattact tccttcctgt tctaatgcct ctgcgatagc ctgaaggtca
    16261 ttcatggcca tccacggaca atgcgcgcag ctgcggcagg ttgcgccctc acccgcggtt
    16321 ggtgcttcca gtaactcttt atctggcacc gcctgctgca ttttgtagaa aataccccga
    16381 tcggttgcca caataagcct ctgatgaggc aatgcttttg cagcagcgat cagttgactg
    16441 gtggaaccga ccgcatccgc catatcgaca atagcttgtg gtgattctgg atgcaccagt
    16501 atggcagcat ccgggtattc ttcttgcaag cgggttaacg cctgagtctt aaattcatca
    16561 tgcacaatgc aggcaccctg ccagcataaa atgtctccac ccgtctgttt ttgcacgtaa
    16621 cgccccagat gtttgtcggg tgcccagatg attttttcac ccaaactatc aagatgatca
    16681 ataagttcga cggcaatgct tgaagttacc acccaatctg cgcgcgcttt taccgcagca
    16741 gaagtgttgg cgtagacgac gacagtacga tcgggatggg catcgtaaaa tgcgttaaat
    16801 tcttcaacag ggcagccgag atccagtgaa cactcaacct gaagtgtcgg catcagaatt
    16861 gttttttccg gactgagaat tttggcggtt tctcccataa atctcacccc agcgactaac
    16921 aaagtagaag cgggatgctt tgcaccgaag cgcgccattt ccagagaatc agaaatacag
    16981 ccaccggttt cttctgccag ttgttgaatt tcgggatcgg tatagtagtg ggcaaccatc
    17041 accgcattac gttcttttag cagacgtttt atcttctcgc ggtaatacgc tttttcatca
    17101 atgcttaacg gcgtcggctt cggggggaaa ggataaatcg ccgtgtctgg atcaaacatt
    17161 acgctcatct taccatctcg ttttacaggc ttaacgttaa aaccgacatt agcgtaacat
    17221 tcgctgttat cggtgtaatt tgttttatat gctaaacaag atagtcgaat ggtggggaaa
    17281 agtcactcga attacgccta attccaccga cgtagagttg cttgcacagt aagctctgac
    17341 gatgtcatca ggtttacaaa gtgaaattga ggatgcctga atagcaaaaa agcgccttta
    17401 gggcgctttt ttgcattggt gggtcgtgca ggattcgaac ctgcgaccaa ttgattaaaa
    17461 gtcaactgct ctaccaactg agctaacgac ccgagtggtg ggtgatgacg ggatcgaacc
    17521 gccgaccccc tccttgtaag ggaggtgctc tcccagctga gctaatcacc cgatactgcg
    17581 ctggatacta caaactactt ccaccgaacc caccgtaatt agtggtgggt cgtgcaggat
    17641 tcgaacctgc gaccaattga ttaaaagtca actgctctac caactgagct aacgacccgg
    17701 tggtgggtga tgacgggatc gaaccgccga ccccctcctt gtaagggagg tgctctccca
    17761 gctgagctaa tcacccgaca ctacgctgga tactacaact aactatgtta gtgggggtcg
    17821 tgcaggatga ctcgacttcg tctcgccctt cggcgccgtt gctgatgcaa cgttatcctt
    17881 cacgttttac taccgcttcc accttagcga ttggtgggtc gtgcaggatt cgaacctgcg
    17941 accaattgat taaaagtcaa ctgctctacc aactgagcta acgacccact ttttcgttgc
    18001 tttcggtttg tttgatatcc cgtggcaacg gcggcatata ttactgattt cagacttgag
    18061 cgcaacaaaa atttcgatgc agatcactca actgcttatg attcgcacga cacgaccaga
    18121 aataatgcga cttctggtcg tgtgttatgc attacatcgc gttcagacgt ttttgtgcct
    18181 gtttagcgcc atcagtacca gggtatttac tgataacctg ctggtatacg gctttcgctt
    18241 ttgcggtgtc acctttgtcc tgcatgatga cgccgacttt aaacatcgca tcagcagcct
    18301 ttggtgactt cggatagttt ttcactaccg aagcaaaata gtacgccgca tcatcttttt
    18361 tacccttgtt gtagtttaac tgacccagcc aataattggc gtttggcagg taagttgaat
    18421 cagggtaatt tttgatgaaa ttctgaaatg ccaccattgc gtcatcctgg cgggatttat
    18481 cctgcaccag cgcaatagct gcattgtaat ccgtgtttgc atcgccgctt ttcaccggcg
    18541 cgccagcatt cgcagtacca gcatcagctg tcggcgtcgt tgatgccgtc gcaccgcttt
    18601 gatcgctgct ggttgattgc gccgctgcac caccgctgct gaggctgtcg atctgcaaca
    18661 ggatctgctt ttgccgctcc acgacctgat tcagttgata ctgattttcc tgaatctgac
    18721 cacgcaggga atcaatatcg gattgattat cagaaagttg ttgctggagt tgggttaaaa
    18781 gctggctgtg agcattagaa atacgctcaa gttgagtgac gcggtcttcg accgagcctg
    18841 agctgacact actgattggt gcctgagcaa aagcggccca gggggccgct ataccaacca
    18901 gtaacgacag actcaatagt tgatgtctga agttactgct catgcaattc tcttagtaaa
    18961 ccagtaccgc acgacggttt ttggagtatg ccgcttcgtc atgacccagt actgcaggtt
    19021 tttctttacc gtaagaaacg atggagatct ggtctgcaga aacgccttta ccctgcaggt
    19081 acatcttaac ggcgttcgca cgacgttcac ccagggagat gttgtattcc ggagtaccac
    19141 gttcgtccgc gtgaccttct acggtgactt tgtaagacgg gttgctacgc aggaagtttg
    19201 catgtgcatc cagcatttga gcgaagtcag aacggatatc gtacttgtcc agatcgaagt
    19261 aaacgatgtt gttctgctgc agctgttgca tttgcagacg agcctgctct tcggaagaca
    19321 tgttgccgtt gccgccgttc gcatccatac cagtgccggc acccagcatg ccttcgctgc
    19381 cgtcattgct ggcgttcttg ttggaagaac atgccgcaat tgccataaca ggcagagcaa
    19441 tcatcagccc tttcagcact ttgttcagtt gcatttcaat gattccttta ctattcaatt
    19501 aattattatc acagatacgg cgaccaggca gggaatttga cctgtccatc agttgccgga
    19561 agacgcgctt tgaaacgccc atctgtagaa accaaattca gcacggatcc catcccctga
    19621 gaagagctgt agattaccat agtgccgtta ggtgccagac ttggcgtttc atccaggaac
    19681 gtggacgaca gaacttgtac gcctcccgtt gccagatctt gtttggcaat gtgctgctgc
    19741 ccaccattgg agctgaccat taccataaat ttaccgtcgc tgctgacatc cgcatcctgg
    19801 ttctgcgaac cttcccaggt aatacgttgt ggcgcaccgc cgttgatatt cactttataa
    19861 acctgcggac gaccggcctg gtcagaagta aatgccaggt tctggctatc cgggaaccag
    19921 gtcggttcgg tattgttact gcgaccatca gtcacctggc ggatctgacc agaagccaaa
    19981 tccattacgt acaggttcag actaccggtt ttcgacaagg cgaatgccag tttgctgccg
    20041 tctggcgaga atgcaggtgc accgttgtga cgcgggaatg aagccacctg acgtacagcg
    20101 ccatttgcca gcgtctgaat aaccagcgcg gaacgaccgc tttcgaaggt cacataagcc
    20161 agtttagaac cgtctggtga ccacgccgga gacatcagcg gctgcggtga acggtgaacg
    20221 acaaactggt tgtaaccgtc atagtcagat acgcgcagtt catacgggaa ctgaccgccg
    20281 ttggtctgaa caacgtaggc aatacgggta cggaacgcac ctttaatgcc ggtcagtttt
    20341 tcaaacactt catcactggc ggtatgacca gcataacgca gccactgctt gttcactttg
    20401 tacgagttct gagcaagtac agtacccggt gcgccgccag tgtcaacaag ttgataagca
    20461 acattgtaag aaccatccgg attcggagtg acttgaccga caactacagc gtcaatgccc
    20521 agtgcggacc atgcagctgg ttgtacttcc tgcgcactac ccggctgctg tggcagacga
    20581 gcgcgatcta acggattaaa tttaccgctg ttacgcaagt cagcagcaac gatgccgcca
    20641 atatcttcag gtgccgcacc aggccccgcc cactggaaag gaacaacacc aataggacga
    20701 ccggaatcta caccgctgtc gatcacaatg cggacttcag catgcagaac tgatgcccac
    20761 agtatgagaa aaccaaatgc tactcgtaat gcctgcttca tcatatctcc cttatctgga
    20821 ccgatggccc acgataattt agcagaatgt taacaaacta aaatacacaa aactaccaga
    20881 accccgtggc aaccggtgcc tgatgttgac cgtccgaaca gtcaacatcg cgattacggt
    20941 ttgaagtcca atggcgcgtt tttgaacact tcatatactg cctggcttgg tggtttcggg
    21001 atcttcgcaa gtttagctgc tgccaacgca gcctgacaaa gtgcgggatc gccaccttca
    21061 ggtttgatat ccagtaacat accatcgggt gccagtttta tgcgcagcgt acaggttttg
    21121 cctgcatagg acgatgcgtc atagaactta ctttcgatag cagatttaat ctgcccggca
    21181 tagttattga tatcggcccc tgatgcgcca ttgtttttag tattaccact cccggcaggc
    21241 gaagcattgt tccctttcgc ccctcccccc gttttcggtg cattcttacc agagcttagc
    21301 tcaccgaaaa tatcatctgc ctctgcggca gcttttgctg cagcggcttt ttcagctgct
    21361 gcttttgccg ctgccgcttt tttgtctgcg gcggcttttt ctgccgctgc tttcttatca
    21421 gctgctgcct tatcagcagc cgctttttct gctgcttttt tgtcggctgc agctttctct
    21481 gctgccgctt tcttatcagc tgcagccttt tcagcagccg ctttcttctc agcttctgcc
    21541 ttggcttttt cagcagcttc agttgccgct ttctttcttg cttcagctgc agctgcttct
    21601 gccgcttccg ctttcttctt cagtgccgca gcggctacct cggctttttt ctgcgcttcg
    21661 gctgcggctt tggcggcttc tgcttctgct tttttcttcg cgtctgcagc cgctttcttc
    21721 gctgcttctt ccgcagcttt agcatctgcc gccgctttcg ctgccgccac ttcagcttgc
    21781 ttctgcttta actcggcctg ttttgcggct tcttcagcct gctttttctg ctcctgagcc
    21841 gctaaccgct ctttctcaag ttgcttcagg cgttcctgtt cagccgcttg tttctcacgc
    21901 agttcttcag cagcctgctg ttccttcatc ttgcgctgtt catcagaacg cttcgcgctt
    21961 gattcctggc tttgcatgcg tttgtactgc tcaactaccg cacctgaatc aaccatgaca
    22021 gcgtcgatgg acgaaccacc gccgcctccg gctgaagctt ctatattctc atcgaacgaa
    22081 ctccagatca gcgccgcaaa taagatgaca tgcagcactg ctgaaataat tatcgcccgc
    22141 ttgagcttgt cgttttgttc ggttgccttt gacactctcg gtttccaaaa actgttcgcc
    22201 tgttacccgc tctctttcaa gcaagggaaa cgcagatgtt tagataggct gcgtcattaa
    22261 accaaccgat ttcacacccg cactatgtaa caagttcagt gctttaatta tttcatcgta
    22321 aggcacatct tttgcgccac cgatcagaaa gaccgttttc gggttggcct tgaaacggct
    22381 ggacacttcc gccaccacct gctctggtgg taaacgctcc aggcgatctt tctcaaccac
    22441 cacggtgtac tgaccaatac cagacacttc aacaatcact ggcggattat cgttactgct
    22501 caccgcctgt gattcagtag cgtctggcag atcgacctcc acgctctggg tgatgatggg
    22561 cgctgtcgcc ataaagatca gcaacagcac cagcagtacg tccagcaacg gaacaatgtt
    22621 gatttcggac ttgagatcgc gacgacctcg tccacgcgct ctggccatgg cttacccctt
    22681 gttgctctcg ctaacggtaa acgcctggcg gtgcagaatc gcggtaaact cttccataaa
    22741 gttgtcgtaa ttcagttcca gtttgtttac gcgctggttg agacggttat aagccattac
    22801 cgccggaatt gcggcgaaca gaccaattgc cgtcgcaatc aacgcttctg cgataccggg
    22861 cgcaaccatt tgcagtgttg cttgttttac cgccccgagg gcgataaagg cgtgcatgat
    22921 cccccagacc gtaccaaaca gaccaatata cgggctgatg gagccaaccg taccgaggaa
    22981 cggaatgtgc gtttccagat tttcaagttc acggttcatg gagatacgca tagcacgcga
    23041 cgccccttcc actacggctt ccggcgcatg gctattggca cgatgcaggc gcacaaactc
    23101 tttgaacccg ctgtaaaaga tttgttccga accagtcaga ttatcccgtt tcccctgact
    23161 ctcttgatag aggcgagaaa gttcgattcc agaccagaat ttatcttcaa acgcttcggc
    23221 ttcacgcgcc gctgcgttaa gaatacgggt tcgctggata ataatggccc aggatgcgat
    23281 tgaaaaacca atcaaaatca acatgataag tttaaccaga aggctagcct tcaggaacaa
    23341 atcaaggata ttcatgtcag tcactgctta aactccgcga caatagactt gggaagcgca
    23401 cgaggcttca ttttgagtgg gtcaacgcaa acaaccagaa cctctgcttc attcagcaaa
    23461 gtattctcgg cgttgacaat acgttgcgtg aaaaccaaag aggtgccacg cattgatgtt
    23521 atttcagtct gtatttcgag catatcgtcg agccgcgcag gtgcgtaata ttccaccgtc
    23581 attttacgta ccacaaaggc aacgcgttca gccatcagcg cctgctgact gaagtgatga
    23641 tgacgcagca tctctgtgcg tgctctttca taaaaagcga cgtaactggc gtggtacacc
    23701 acaccaccgg catcggtatc ttcatagtag acgcgaaccg gccatcgaaa cagcgttgta
    23761 ttcactttac atcccggtaa tgcaacaaaa gttagagctt ttaaacgctg ctactatacg
    23821 cgcgcgattg aggtttggga atgagggcaa gttaagggcg cgtaaaaatt tatgggccta
    23881 gagcggccca taatttttta aagagaattc tgatttaaaa gaagaagaaa atcagcccga
    23941 caatgagaac aatatcggca agcaatgggc aaaaaatccc ttgccaaaga accttctgcg
    24001 gacgaaagcc cacgccgtga atcacaccag cacagacggc ccacatcagc aataaaccat
    24061 gccagatttc cagttcactg gtcttcgcgg caaaacgaga tgggtcccaa aacatacatc
    24121 ctgctaacag aagcgccatc acgaaggaaa gcgcccgcag ggggcgcttg tccattaccg
    24181 catacaaggt cgcgataatc ttactcatca gatgtcttct tgaccggctt tgcctgattc
    24241 gacgtgttca agcgccagcg cggtgattac cccaaacgaa caggcaagaa gcgttcccag
    24301 aatccatgcg aaataccaca tttttagctc cttacttagt acagagagtg ggtgttacgt
    24361 tcaatatctt ctttggtgat acgaccgaac attttccagt aacaccaggc ggtgtagagc
    24421 agaatgatcg gtaccagaac caccgcaacc caggtcatga cgttaagcgt cagctggctg
    24481 gaagttgcat cccacattgt cagacttgcg ttcatcatgg tgctggacgg catcacaaac
    24541 gggaacattg cgataccggc tgtcaggatg atgcaggcca gcgtcaggga ggagaacaca
    24601 aacgcccacg cggctttatc catacgtgca gtcaggatgg tcagcagcag cagaaccaca
    24661 cccagtgccg gaatagccca cagaattggc gtgttgttga agttaaccag ccatgcgcca
    24721 gcttcacgaa ccacttcttt attcagtggg ttagaggctg cgtaatggtc cattgtcgat
    24781 ttcacgacat aaccatcgat accgtacatc acccatacgc cagccagtgc gaaacagacc
    24841 agtgtcacca gcgcagccac ctgagccgtt gcacgggtac gcaggtgcag ttcgcccacg
    24901 gtacgcattt gcagataggt tgcgccctga gtaatgatca tccctacgct caccacgcct
    24961 gccagcaggc cgaacgggtt aagcaactgg aagaagttac cggtgtagta cagacgcaga
    25021 tattcatcaa cgttgaacgg tacgccctgc aacaggttac cgaacgctac accaattacc
    25081 agcggcggaa cgaagctacc aatgaagatg ccccagtccc acatgttacg ccagcgggtt
    25141 tcttcaatct tggagcggta gtcaaaaccg accggacgga agaacaaaga cgccagcacg
    25201 aggatcatcg ccacatagaa gccggagaac gcagcggcat agaccatcgg ccaggcagca
    25261 aagagtgcgc cgcccgcggt gatcagccaa acctggttac cgtcccagtg tggtgcaatg
    25321 gagttaatca taattcgacg ctcggtgtcg ttacgaccga ggaaacgggt gagcatgccc
    25381 acccccatgt cgaaaccgtc agtgactgca aaaccaatca gcagaacgcc aaccagcagc
    25441 caccagataa aacgcaatac ttcataatcg atcatttgac gactcctgtc ttagcgtgcc
    25501 ggctgagtag tcgtggaaga ctgctcaaag tgatagcgac cggttttcag gctgcttggg
    25561 ccgaggcgtg caaacttgaa cattaagaac aattctgcca ccaggaacag ggtatacagg
    25621 ccgcaaatca gcaccattga gaagatgaga tcgcctgcgg tcagtgacga attcgccaca
    25681 gctgtcggca gcacttcacc gatagcccac ggttggcggc catattcagc cacgaaccag
    25741 cccgcttcta cagcaatcca cggcagcgga ataccgtaca gcgcggcgcg cagaagccat
    25801 tttttctcgc caatgcggtt gcggatgaca ctccagaaag agagcgcgat gattgccagc
    25861 agcaggaagc cacacgccac catgatacgg aacgcaaagt acagcggcgc tacacgcggg
    25921 atggagtctt tggttgcctg ttgaatctgc gcttcagtcg catcagccac gtttggcgta
    25981 tagcgtttca gcagcagacc gtaaccgagg tctttcttca tgctattgaa ctggtcacga
    26041 accgtctggt cggtagaacc agaacgcagt tgttcgagca gagagtacgc cttcatcccg
    26101 ttacgaatgc gttcttcatg ctgcaccatc agctctttca ggccgataac cggggtatcc
    26161 acggaacgcg ttgcaatgat gcccagtgcg taagggatct gaatcgcaaa tttgttcgtc
    26221 tcctcttcct gatcaggaat gccgaacaaa gtaaaggcag caggcgcagg ttgcgtttcc
    26281 cactcggctt caatagcagc cagtttggtt ttctgcacgt cgcccatttc gtagccggat
    26341 tcatcaccca gaacaataac agacagaaca gcagccatac cgaagctggc agcgatagca
    26401 aaggagcgtt tagcgaaggc gaagtcacga cctttcagca tataccatgc gctgataccg
    26461 aggatgaaca tcgcgccagt cacataacca gacgctacag tgtgaacgaa tttcacctga
    26521 gcaaccgggt taagcaccag ctcggagaag ctcaccatct ccatacgcat agtttcaaag
    26581 ttgaaatcgg acgcgattgg gttttgcatc cagccgttcg caaccagaat ccacagtgcg
    26641 gacaggtttg aaccgagcgc caccagccag gtgacacaca tatgctgaac tttacccaga
    26701 cgatcccaac cgaagaagaa cagacctaca aaggtggatt cgaggaagaa ggccatcaga
    26761 ccttcgattg ccagcggcgc accgaagata tcccctacat agtgggaata gtaagaccag
    26821 ttagtcccga actggaactc catggtcaga ccggtagcca cacccagagc gaagttgata
    26881 ccaaacaact tgccccagaa cttggtcata tctttataaa tctgtttgcc ggagaggacg
    26941 tagaccgttt ccataatggc caggaggaac gccataccga gcgtcagtgg cacaaaaagg
    27001 aagtggtaca tcgcggtcaa ggcaaactgt aagcgcgaca gttcgactat atctaacatc
    27061 atgactcctt gctcatcgca tgaagactcc gagagtgaac cccgttagaa agggtcacac
    27121 gcatgcccca atagaaattt gtttgaacac tttcatcata ccatgtgaat gcacacaaaa
    27181 acggcgtaaa gaaaggttgc aaacatgtta ataaaaactc aaattgatcc cacgtatata
    27241 ttacgccgca aaatccttac aataaacagg tttttgttga agcaaatttg catttttcga
    27301 cggtgatcaa tttatagcta aattgccgcc tttcagccaa tttgatcgag aacaatttat
    27361 ctctttttga tgcccatttc caa
//
LOCUS       DAZPCS010000038        27243 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZPCS010000038 DAZPCS010000000
VERSION     DAZPCS010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27243
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.038"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(189..1853)
                     /gene="glnS"
                     /locus_tag="ACHUQ7_001623"
     CDS             complement(189..1853)
                     /gene="glnS"
                     /locus_tag="ACHUQ7_001623"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFR1135085.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHITVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(2056..4002)
                     /gene="nagE"
                     /locus_tag="ACHUQ7_001624"
     CDS             complement(2056..4002)
                     /gene="nagE"
                     /locus_tag="ACHUQ7_001624"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFR1135086.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLLIKAQGHVVAGQTPLYEIKK"
     gene            4335..5135
                     /gene="nagB"
                     /locus_tag="ACHUQ7_001625"
     CDS             4335..5135
                     /gene="nagB"
                     /locus_tag="ACHUQ7_001625"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFR1135087.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMIMVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5195..6343
                     /gene="nagA"
                     /locus_tag="ACHUQ7_001626"
     CDS             5195..6343
                     /gene="nagA"
                     /locus_tag="ACHUQ7_001626"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFR1135088.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            6352..7572
                     /gene="nagC"
                     /locus_tag="ACHUQ7_001627"
                     /gene_synonym="nagR"
     CDS             6352..7572
                     /gene="nagC"
                     /locus_tag="ACHUQ7_001627"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFR1135089.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7620..8372
                     /gene="nagD"
                     /locus_tag="ACHUQ7_001628"
                     /pseudo
     CDS             7620..8372
                     /gene="nagD"
                     /locus_tag="ACHUQ7_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
     gene            complement(8423..8521)
                     /locus_tag="ACHUQ7_001629"
                     /pseudo
     CDS             complement(8423..8521)
                     /locus_tag="ACHUQ7_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8769..10433
                     /gene="asnB"
                     /locus_tag="ACHUQ7_001630"
     CDS             8769..10433
                     /gene="asnB"
                     /locus_tag="ACHUQ7_001630"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFR1135090.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKNHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNVKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            10812..10888
                     /locus_tag="ACHUQ7_001631"
     tRNA            10812..10888
                     /locus_tag="ACHUQ7_001631"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10846..10848,aa:Met,seq:cat)
     gene            10897..10981
                     /locus_tag="ACHUQ7_001632"
     tRNA            10897..10981
                     /locus_tag="ACHUQ7_001632"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10931..10933,aa:Leu,seq:tag)
     gene            11005..11079
                     /locus_tag="ACHUQ7_001633"
     tRNA            11005..11079
                     /locus_tag="ACHUQ7_001633"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11037..11039,aa:Gln,seq:ttg)
     gene            11114..11188
                     /locus_tag="ACHUQ7_001634"
     tRNA            11114..11188
                     /locus_tag="ACHUQ7_001634"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11146..11148,aa:Gln,seq:ttg)
     gene            11204..11280
                     /locus_tag="ACHUQ7_001635"
     tRNA            11204..11280
                     /locus_tag="ACHUQ7_001635"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11238..11240,aa:Met,seq:cat)
     gene            11329..11403
                     /locus_tag="ACHUQ7_001636"
     tRNA            11329..11403
                     /locus_tag="ACHUQ7_001636"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11361..11363,aa:Gln,seq:ctg)
     gene            11441..11515
                     /locus_tag="ACHUQ7_001637"
     tRNA            11441..11515
                     /locus_tag="ACHUQ7_001637"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11473..11475,aa:Gln,seq:ctg)
     gene            complement(11669..12844)
                     /gene="ubiF"
                     /locus_tag="ACHUQ7_001638"
     CDS             complement(11669..12844)
                     /gene="ubiF"
                     /locus_tag="ACHUQ7_001638"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFR1135091.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPTRIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            12990..14414
                     /gene="miaB"
                     /locus_tag="ACHUQ7_001639"
     CDS             12990..14414
                     /gene="miaB"
                     /locus_tag="ACHUQ7_001639"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFR1135092.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            14567..15607
                     /locus_tag="ACHUQ7_001640"
     CDS             14567..15607
                     /locus_tag="ACHUQ7_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFR1135093.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            15604..16071
                     /gene="ybeY"
                     /locus_tag="ACHUQ7_001641"
     CDS             15604..16071
                     /gene="ybeY"
                     /locus_tag="ACHUQ7_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFR1135094.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            16161..17039
                     /gene="corC"
                     /locus_tag="ACHUQ7_001642"
     CDS             16161..17039
                     /gene="corC"
                     /locus_tag="ACHUQ7_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFR1135095.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            17064..18602
                     /gene="lnt"
                     /locus_tag="ACHUQ7_001643"
     CDS             17064..18602
                     /gene="lnt"
                     /locus_tag="ACHUQ7_001643"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFR1135096.1"
                     /translation="MDFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIATPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     AEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFAREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            18999..19907
                     /gene="gltI"
                     /locus_tag="ACHUQ7_001644"
     CDS             18999..19907
                     /gene="gltI"
                     /locus_tag="ACHUQ7_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFR1135097.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            20077..20817
                     /gene="gltJ"
                     /locus_tag="ACHUQ7_001645"
     CDS             20077..20817
                     /gene="gltJ"
                     /locus_tag="ACHUQ7_001645"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFR1135098.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWVWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQTYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            20817..21491
                     /gene="gltK"
                     /locus_tag="ACHUQ7_001646"
     CDS             20817..21491
                     /gene="gltK"
                     /locus_tag="ACHUQ7_001646"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFR1135099.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            21491..22216
                     /gene="gltL"
                     /locus_tag="ACHUQ7_001647"
     CDS             21491..22216
                     /gene="gltL"
                     /locus_tag="ACHUQ7_001647"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFR1135100.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMAPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            22334..23269
                     /gene="rihA"
                     /locus_tag="ACHUQ7_001648"
     CDS             22334..23269
                     /gene="rihA"
                     /locus_tag="ACHUQ7_001648"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFR1135101.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            23353..25023
                     /gene="hscC"
                     /locus_tag="ACHUQ7_001649"
                     /pseudo
     CDS             23353..25023
                     /gene="hscC"
                     /locus_tag="ACHUQ7_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
     gene            complement(25083..26534)
                     /gene="djlC"
                     /locus_tag="ACHUQ7_001650"
     CDS             complement(25083..26534)
                     /gene="djlC"
                     /locus_tag="ACHUQ7_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HFR1135102.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(26531..27238)
                     /locus_tag="ACHUQ7_001651"
     CDS             complement(26531..27238)
                     /locus_tag="ACHUQ7_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR1135103.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRAGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
BASE COUNT         6665 a         7056 c         6929 g         6593 t
ORIGIN      
        1 tgctatttct tgccggatgc ggcgtgaacg ccttatccgg cctacaaaag cacgcaaatt
       61 caatatattg cagagatcat gtaggcctga taagcgaagc gcatcaggca atttagcgtt
      121 tgaaatttat tgtctattta ttgccggatg cgacgtaaat gcctcacccg gcttggtaga
      181 acttaaaatt actcgcctac tttcgcccag gtatcacgca gcccaacggt gcggttaaat
      241 accggttttt cggccgtgga atggcggctg tcgaggcaga agtaaccttc acgctcaaac
      301 tggaatgctt tacccgcaac cgcatctttc agcgacggtt cagcaaagcc ctgtttgatc
      361 accagcgatt ccgggttaat caccgacagg aaatcatccg cagcacctgg gttaggcacg
      421 ctgaacagac gatcatacaa acggatttca accggcagcg catgtgccgc gctcacccag
      481 tgaataacac ctttgacttt acgaccatct gccggatctt tgcttaaggt gtcggcgtca
      541 taagtacaga agatggtggt gatattacct tcggcatctt tctccacgcg ttcagcctta
      601 atgacgtaag cattacgcag acgcacttct ttacccagca ccagacgttt gtactgcttg
      661 ttagcttctt cgcggaaatc ggcgcgatcg atccaaattt caccgctaaa cggcacctga
      721 cggctcccca tttccggttt gttcggatgg ttcggcatgg tgaccatttc gccttcgccc
      781 tgatagtttt cgataaccag tttcaccgga tcgataaccg ccattgcgcg cggcgcattt
      841 tcgttgagat cttcacggat gcaggattcc agcgacgcca tctcaatggt gttgtcctgc
      901 ttggtcacgc cgatgcgttt gcagaactca cgaatagaag ccgcagtgta accacgacga
      961 cgcagaccgg aaatggtcgg catacgcggg tcatcccagc cttcaacgtg cttgtcggtc
     1021 accagcaggt tcagcttacg cttggacatc acagtgtatt ccagattcag gcgcgagaat
     1081 tcgtactggc gcggatgaac aggaatggtg atgttatcca gtacccagtc gtacagacga
     1141 cggttgtcct ggaactcaag cgtacacaga gagtgcgtaa taccttccag cgcatcgctg
     1201 atgcaatggg tgaagtcgta catcgggtag atgcaccact tgttgccagt ctggtggtgt
     1261 tcagcaaatt taatgcggta cagcaccgga tcgcgcatta cgataaatgg cgacgccatg
     1321 tcgattttcg cacgcaggca ggctttacct tcttcaaaac caccggcacg cattttttcg
     1381 aacagcgcca agttctcttc aacgctgcgg tcgcggtacg ggctgttttt acccggctgc
     1441 gtcagggtac cgcggtattc gcggatctgt tccggtgtca gttcatcaac gtacgccagg
     1501 cctttattga tcagttcgat cgcataggcg tggagctgat caaaataatc ggaggagtaa
     1561 cggacgttac cagaccagtg aaaacctaac cactctacgt cgtttttgat cgaatcgaca
     1621 tactcgatat cttcttttac cgggttagtg tcgtcgaaac gcaggttgca ctggccttta
     1681 tagtcctggg cgatcccgaa gttcaggcag atagatttcg catggccaat atgcagatag
     1741 ccattcggct ccggcgggaa acgggtgtgt actgtgatgt gcttaccact ggccagatct
     1801 tcatcgatga tctgacggat aaagttagtc gggcgggctt ctgcctcact catcgtggat
     1861 tcctcaaagc gtaaacaacg tataacggcg tatgatctta taagcgggac aggctgacaa
     1921 ctgttagttt tttaaaattg ataatctaac aaggattatg gggatgattg atgaaaaaaa
     1981 aagcggcaga gaagatctcc accgcttgtc attgttggat gcgacgctgc cgtgtcgcat
     2041 caggcataaa gcagattact ttttgatttc atacagcggt gtttgacccg ccacaacatg
     2101 gccctgagct ttaatgagca agccactgaa atcgtcgata ttgctgcaaa ccaccgggct
     2161 aatcatcgag cgggcgttag cgttcaggta atccagatcc atttccagaa tcggttgccc
     2221 tgcgcttacc tgcgcaccct cttccaccag acgtttaaag cctttacctt ccagcgctac
     2281 ggtgtcgata cccatatgga cgacgatctc cgcgcctttt tcggtttcca ggcagaacgc
     2341 gtggttggtg ttgaagattt tcacgattgt ccctgcggct ggtgatacga cgattttatc
     2401 tgtcggtttc accgccacac cgtcacccac cgctttgctg gcgaatgctt cgtcaggaac
     2461 ctgatccagt gccacgacat caccggtaat cggcgatacc agctccgcga tagataccgc
     2521 gtttggtaca gcctgcggtt ttgctacagg cgcggcagtt gccggagttg cttcagctga
     2581 cgcagcggct accggaccac gggcaacgac tttcttcatc gcatcgccga tggattctgc
     2641 tttcgcgcca acaatcacct gaatagtctg tttgttcagt ttcactacac cagaagcacc
     2701 cagacgttta cacatcgtat cgttgacgcg ggcagagtca gccacggtca ggcgcagacg
     2761 ggtaatacag gcatcaatcg ctttcaggtt gtcagtaccg ccaaccgcag caatatagtt
     2821 ggttgccagt tgagtcagac cttcttcagt attgctgttg gcttcttcag taacgatctc
     2881 gtcttcttta tcttcacgac ccggcgtttt caggttgaac atgcggataa ccaaactgaa
     2941 caccacgaag tagatagcga agaagataac gcccatcacc agcagcatcc agacgttctg
     3001 gctggcggca ggcaggttat acatcaacgc gtagtcgata gcacccgcag agaaagagaa
     3061 gccggcatgg atacccagca gcgttgccac aaacaggctg ataccggtca gcagtgcgtg
     3121 caggaggtac agcagcggcg caaggaacat gaacaggaat tccagcggct cagtcacacc
     3181 ggtcaggaac gcagtaacag caacagaaag cagcatcccg ccaaccatcg gacgacgctc
     3241 tttcggtgct gcgaagtaca tcgccagcgc cgcacccggc agaccgaaca tcatgatcgg
     3301 gaagaagccg gacatgaaca tccccgcggt gccgtcacca gcgtagaaac ggttgatgtc
     3361 gccgtggaaa accgtacccg ccgcgttggt gaattcacca atctggaacc aggcgatggt
     3421 gttcagcacc tgatgcagac cggttgggat cagcagacgg ttgatgaaac caaagatacc
     3481 ggaacccagc gcgcccgcag aaacgatcca ctcgccgcct gcatggatag cgtgctgtac
     3541 cggcggccag acgtaaccaa aaatggccgc cagcaccaga cagaagaagc cggtggcgat
     3601 cggcacaaag cgtttgccgc cgaagaagct caggaagtcc ggcagtttaa tatcggacca
     3661 acggttatag gctgcgccac caaccagacc ggtaatgata cccgccagta cacccatgtt
     3721 aatttctggg ttgatggtca ccatcgcttt ggttaacaca aagtaaccta ccgcacccgc
     3781 cagcgccgcc gcacctgcgc tgtctttcga ccagctggat gccacaccga tggcgaagat
     3841 taatgcgagg ttatcaaaaa ttgcaccgcc cgcctgggca ataaacgcaa cgttaagtaa
     3901 atctggctga ccgaatcgca gcaacagcgc cgccaccggc agcaccgcga tagggagctg
     3961 taacgcccta ccgagtcgct ggaaaaaacc taaaatattc atcttattcc ccctacgaga
     4021 accctatttg gctcgtttaa agccgtattt ttattttgct gcaaatttta ctgccgatgt
     4081 tctgtaatca gattgttaga tcatctgcta cagagtgtgt gaaaatttaa ttcgcatcgc
     4141 aaattaaacg cgtgtctttt gtgagttttg tcaccaaata tcgttatcat cactcccttt
     4201 tactggctaa accagaaaac ttattttatc attcaaaaaa tcaagtcgga ttgacgcctg
     4261 tctgcgcaaa tccaggttac gcttaaagat gtctaatccg ccaacggctt acattttact
     4321 tattgaggtg aataatgaga ctgatccccc tgactaccgc tgaacaggtc ggcaaatggg
     4381 ctgctcgcca tatcgtcaat cgtatcaatg cgttcaaacc aactgccgat cgtccgtttg
     4441 tactgggcct gccgactggc ggcacgccga tgaccaccta taaagcgtta gtcgaaatgc
     4501 ataaagcagg ccaggtcagc tttaagcacg ttgtcacctt caacatggac gaatatgtcg
     4561 gtctgccgaa agagcatccg gaaagctact acagctttat gcaccgtaat ttcttcgatc
     4621 acgttgatat tccagcagaa aacatcaacc ttctcaacgg caatgccccg gatatcgatg
     4681 ccgagtgccg ccagtatgaa gaaaaaatcc gttcttacgg aaaaattcat ctgtttatgg
     4741 gcggtgtagg taacgacggt catattgcat ttaacgaacc ggcgtcttct ctggcttctc
     4801 gtactcgtat caaaaccctg actcatgaca ctcgcgtcgc gaactctcgt ttctttgata
     4861 acgatgtcaa tcaggtgcca aaatatgccc tgactgtcgg tgttggtaca ctgctggatg
     4921 ccgaagaagt gatgattatg gtgctgggta gccagaaagc actggcgctg caggccgccg
     4981 ttgaaggttg cgtgaaccat atgtggacca tcagctgtct gcaactgcat ccgaaagcga
     5041 tcatggtgtg cgatgaacct tccaccatgg agctgaaagt taagacttta agatatttca
     5101 atgaattaga agcagaaaat atcaaaggtc tgtaattgtt atccctgccc tggttccttg
     5161 ctcagggcaa tattttttaa aatcgggggg cagaatgtat gcattaaccc agggccggat
     5221 ctttaccggc cacgaatttc ttgatgacca cgcggttgtt atcgctgatg gcctgattaa
     5281 aagcgtctgt ccggtagcgg aactgccgcc agagatcgaa caacgttcac tgaacggggc
     5341 cattctctcc cccggtttta tcgatgtgca gttaaacggc tgcggcggcg tacagtttaa
     5401 cgacaccgct gaagcggtca gcgtggaaac gctggaaatc atgcagaaag ccaatgagaa
     5461 atcaggctgt actaactatc tgccgacgct tatcaccacc agcgatgagc tgatgaaaca
     5521 gggcgtgcgc gttatgcgtg agtacctggc aaaacatccg aatcaggcgt taggtctgca
     5581 tctggaaggt ccgtggctga atctggtaaa aaaaggcacc cataatccga attttgtgcg
     5641 taagcctgat gccgcgctgg tcgatttcct gtgtgaaaac gccgacgtca ttaccaaagt
     5701 gaccctggca ccggaaatgg ttcctgcgga agtcatcagc aaactggcaa atgccgggat
     5761 tgtggtttct gccggtcact ccaacgcgac gttgaaagaa gcaaaagccg gtttccgcgc
     5821 ggggattacc tttgccaccc atctgtacaa cgcgatgccg tatattaccg gtcgtgaacc
     5881 gggcctggcg ggcgcgatcc tcgacgaagc tgacatttat tgcggtatta tcgctgatgg
     5941 cctgcatgtt gattacgcca acattcgtaa cgctaaacgc cttaaaggcg acaaactgtg
     6001 tctggttacc gacgccaccg cgccagcagg tgccaacatt gaacagttca tttttgcggg
     6061 taaaacaata tactaccgta acggactttg tgtggatgag aacggtacgt taagcggttc
     6121 atccttaacc atgattgaag gcgtgcgtaa tctggtcgaa cattgcggta tcgcactgga
     6181 tgaagtgctg cgtatggcga cgctctatcc ggcgcgtgcg attgacgttg aaaaacgtct
     6241 cggcacgctc gccgcaggta aagtagccaa cctgaccgca ttcacacctg attttaaaat
     6301 caccaagacc atcgttaacg gtaacgaggt cgtaactcaa taagagaaag tatgacacca
     6361 ggcggacaag ctcagatagg taatgttgat ctcgtaaaac agcttaacag cgcggcggtt
     6421 tatcgcctga ttgaccagta cgggccaatc tcgcggattc agattgccga gcaaagccag
     6481 cttgcccccg ccagcgtaac caaaattacg cgtcagctta tcgaacgcgg gctgatcaaa
     6541 gaagttgatc agcaggcctc caccgggggc cgccgcgcta tctccatcgt caccgaaacc
     6601 cgcaatttcc acgcaatcgg cgtacggctt ggtcgtcatg acgccaccat cactctgttt
     6661 gatctcagca gcaaagtgct ggcagaagaa cattacccgc tgccggaacg tacccagcag
     6721 acgctggaac atgccctgct gaatgccatt gctcagttta ttgatagcta ccagcgcaag
     6781 ctgcgcgagc taatcgccat ttcggtgatc ctgccagggc ttgttgaccc ggacagcggc
     6841 aaaattcatt acatgccgca tattcaggta gaaaactggg ggctggtaga agcactggaa
     6901 gaacgtttta aagtgacctg tttcgttggt cacgatatcc gtagtctggc gctggcggag
     6961 cactacttcg gtgcaagtca ggattgcgaa gactccattc tggtgcgtgt ccatcgcgga
     7021 accggggccg ggattatctc taacgggcgc atttttattg gccgcaacgg taacgtcggt
     7081 gaaattggcc atattcaggt cgaaccgctg ggtgaacgct gccactgcgg caactttggc
     7141 tgcctggaaa ctatcgccgc caacgctgcc attgaacaac gggtgttgaa tctgttaaag
     7201 cagggctacc agagccgtgt gccactggac gactgcacca tcaaaactat ctgcaaagcc
     7261 gcgaacaaag gcgatagcct ggcctcggaa gtgattgagt atgtcggtcg tcatctgggt
     7321 aaaaccatcg ccattgctat caacttattt aatccgcaaa aaattgttat tgctggtgaa
     7381 atcaccgaag ccgataaagt gctgctccct gctattgaaa gctgcattaa tacccaagcg
     7441 ctgaaggcgt ttcgtactaa tctgccggtg gtacgttctg agctggatca ccgctcggca
     7501 atcggcgctt ttgcgctggt aaaacgcgcc atgctcaacg gtattttgct ccagcatttg
     7561 ctggaaaatt aatgtgcttt tatagtggcg cttattgttg tcaatattct gggtagtcca
     7621 tgaccattaa aaatgtaatt tgcgatatcg acggcgtgct gatgcacgat aacgtcgccg
     7681 taccgggtgc agcggaattt ttgcacggga ttatggataa aggcctgccg ctggtgttgc
     7741 tgaccaacta tccttcgcag actgggcaag atctggcgaa ccgctttgcc actgccggtg
     7801 tcgatgtgcc ggatagcgtg ttttatacct ctgcgatggc gaccgccgat ttcctgcgtc
     7861 gctaggaagg caagaaagcg tatgtggtgg gcgaaggcgc actgattcat gaactgtaca
     7921 aagccggttt cactattacc gatgtgaacc ctgatttcgt gattgttggc gaaacacgtt
     7981 cctacaactg ggacatgatt cataaagcag cctatttcgt cgctaacggt gcacgcttta
     8041 tcgccaccaa cccggacacc cacgggcgcg gtttttatcc cgcttgtggc gcgttgtgtg
     8101 cagggattga gaaaatctcc gggcgcaaac cgttctatgt tggtaagccc agcccgtgga
     8161 tcatccgcgc ggcattaaac aaaatgcagg cgcattcgga agaaacagtg attgtcggcg
     8221 ataacctgcg taccgatatt ctggcgggct tccaggccgg tctggagacg attctggtgc
     8281 tttctggtgt ttcatcgctc gacgatatcg acagtatgcc gttccgccca agctggattt
     8341 acccgtcagt cgctgaaatc gacgttatct gaataccacc cacgtctgcg gacgtggttg
     8401 gctctgatga caaacgccaa actgcctgat gcgctacgct tatcaggcct acgcagctcc
     8461 tgcaatatat tgaattttca tgcttttgta ggccggataa ggcgttcacg ccgcatccgg
     8521 catgaacaaa tcgcacttta tcagcaattt ggcccccgta aaatataacc attcaataaa
     8581 acccgctaaa aattatcgat tcatcaataa atacatcctc acaccagacc ctttttcacc
     8641 aatcgacaat caccattacg ttttttattt ttgcactgcc aaatagcttg cgcacagtgc
     8701 tgctttgcgg cattttttta aacaagcaaa cacaacaagc aacaaatacc aggttaacgg
     8761 agaaggttat gtgttcaatt tttggcgtat tcgatatcaa aacagacgca gttgagctgc
     8821 gtaagaaagc actcgagctg tcacgcctga tgcgtcatcg tggcccggac tggtccggta
     8881 tttatgccag cgataacgcc attctcgctc acgaacgtct gtcaattgtt gacgttaacg
     8941 cgggggcgca acctctctac aaccaacaaa aaaaccacgt actggcggta aacggtgaaa
     9001 tctacaacca ccaggcattg cgcgctgaat atggcgatcg ttatcagttc cagacagggt
     9061 ctgactgtga agtgatcctc gcgctgtatc aggaaaaagg gccggaattt ctcgacgact
     9121 tgcagggcat gtttgccttt gccctgtacg acagcgaaaa agatgcctac ctgattggtc
     9181 gcgaccatct ggggatcatc ccactgtata tggggtatga cgaacacggt cagctgtatg
     9241 tggcctcaga aatgaaatcg ctggtgccag tttgccgcac gattaaagag ttcccggcgg
     9301 ggagctattt gtggagtcag gacggcgaaa tccgttctta ttatcatcgc gactggttcg
     9361 actttgatgc agtgaaagat aacgtgaccg acaaaaacga gctgcgtcag gcactggaag
     9421 attccgttaa aagccatctg atgtctgatg tgccttacgg tgtgctactt tctggtggtc
     9481 tggattcctc aattatttcc gctatcacca agaaatacgc agcccgtcgc gtggaagatc
     9541 aggaacgctc tgaagcctgg tggccgcagt tacactcctt tgctgtaggt ctgccgggtt
     9601 caccggatct taaagcagcc caggaagtgg caaaccatct gggcacggtg catcacgaaa
     9661 ttcacttcac tgtacaggaa ggtctggatg ccatccgcga cgtgatttac cacatcgaaa
     9721 cttatgatgt gaccactatt cgcgcttcaa caccgatgta tttaatgtcg cgtaagatca
     9781 aggcgatggg cattaaaatg gtgctgtccg gtgaaggttc tgatgaagtg tttggcggtt
     9841 atctttactt ccataaagcg cccaacgtta aagaactgca tgaagagacg gtgcgtaaac
     9901 tgctggcctt gcatatgtat gactgcgcgc gcgccaacaa agcgatgtca gcctggggcg
     9961 tggaagcacg cgttccgttc ctcgacaaaa aattccttga tgtggcgatg cgcattaacc
    10021 cgcaggataa aatgtgcggt aacggcaaaa tggaaaaaca catcctgcgt gaatgttttg
    10081 agtcatacct gcccgcaagc gtggcctggc ggcagaaaga gcagttctcc gatggcgtcg
    10141 gttacagttg gatcgacacc ctgaaagaag tggcggcgca gcaggtttct gatcagcaac
    10201 tggaaactgc ccgcttccgc ttcccgtaca acacgccgac ctcaaaagaa gcgtatctgt
    10261 accgcgagat ctttgaagaa ctgttcccac ttccgagtgc cgctgagtgc gtgccgggcg
    10321 gtccttccgt cgcgtgttct tcagctaaag cgatcgaatg ggatgaagcg ttcaaaaaaa
    10381 tggacgatcc gtctggtcgt gcggttggcg ttcaccagtc ggcgtataag taagaaatgc
    10441 ggtactgccc cggagaaatc cggggctgtt ttagttattg cgctgcttgt tgattctgat
    10501 atgcagtaag gaaatgttcc gggtttgcca catcactgtt tgacaactca tcactgtagg
    10561 ccagtatttc atagattaat ttcccaccac tattgttgaa ttccgaattt catcctgtac
    10621 gacaaaagaa tggctgtagg gtggggaaaa acacaaagta aacaataatt gacgaatata
    10681 gcgccacgct gttcgcaacc taaccaaaca gtcactttcg agcaattttc cttgaaaaag
    10741 aggttgacgc tgcaaggctc tatacgcata atgcgccccg caacgccgat aaggtatcgc
    10801 gaaaaaaaga tggctacgta gctcagttgg ttagagcaca tcactcataa tgatggggtc
    10861 acaggttcga atcccgtcgt agccaccatc ttttttgcgg gagtggcgaa attggtagac
    10921 gcaccagatt taggttctgg cgccgcaagg tgtgcgagtt caagtctcgc ctcccgcacc
    10981 attcaccaga aagcgttgta cggatggggt atcgccaagc ggtaaggcac cggtttttga
    11041 taccggcatt ccctggttcg aatccaggta ccccagccat cttcttcgag taagcggttc
    11101 accgcccggt tattggggta tcgccaagcg gtaaggcacc ggtttttgat accggcattc
    11161 cctggttcga atccaggtac cccagccatc gaagaaataa tctggctacg tagctcagtt
    11221 ggttagagca catcactcat aatgatgggg tcacaggttc gaatcccgtc gtagccacca
    11281 aattctgaat gtgtcgaata cgttcggcaa attcaaaaac caatttgttg gggtatcgcc
    11341 aagcggtaag gcaccggatt ctgattccgg cattccgagg ttcgaatcct cgtaccccag
    11401 ccaatttatt caagacgctt acatcgtaag tgcacccagt tggggtatcg ccaagcggta
    11461 aggcaccgga ttctgattcc ggcattccga ggttcgaatc ctcgtacccc agccacatta
    11521 aaaaagctcg cttcggcgag ctttttgctt ttctgcgttc attcaatgtc gaatgcgatg
    11581 ttgacacgtc ttatccttca atgtcggatg cgacgctgcc gcgtcttatc cgacctacgg
    11641 ctggcacgca tccggcaatg ttgtaaggct acaaccctaa cgcatatttc agcgcctgac
    11701 gtttcaacac gccagcacgc tctgccgcca ttaacccgag attacgcaca aaacgcagcg
    11761 gcggcagatt attactgaat ccggcataaa acagatccat accactttgc ataatgaagt
    11821 tatccgccat gcgccgcatc tggtaacgct tgaggacagg ataactggcc cacgcttcac
    11881 cgtagctgcg ggcattcacc agaacatcaa tcagggcatc gacatcacga taaccaagat
    11941 tcaccccctg ccccgccagc ggatggatgg tatgcgcggc atcgcccacc agcgcaagcc
    12001 ccggctgcac atactgcaac gcatgtcggc gcgtcagcgg aaacgcacca gcggcaagcg
    12061 gtgtaacgta acccagacgc gacgggaaat gcttcgcgat ttccgcctgg agttgcgcca
    12121 tattcatatt ctgcaactgg cgaatacgcg tcggagagtc ataccacacc agcgatgccc
    12181 agttatcaaa caacggcaga aacgcacgcg gtccgtccgg ggtaaattgc tgccaggtgc
    12241 tgtcgccggg atcgttctca cactgtacgc taatcaacat gcacgactgc gcatactgcc
    12301 aggcatgaac gccaattccc gccatctgcc gcacctgaga atttgcgccg tcggcaccaa
    12361 tcaccagttt cgcgcggatc gtttcaccgc ccttcagttc cagctcctga agatcattat
    12421 ctcgatgtaa tgcaatcagc gaggttggca cacgtaacgt tactttcgga tgcgcttcca
    12481 gcgcctgcca caacgcctgt tgcagcactg tgttttccac catgtagcca agcaacggca
    12541 actttaattc ggctgcgtca aataccacat gtgccgtttc ccactcccag gtttccagtc
    12601 tgcggtaagg atggcaacgc atagcctgta ctgcatccca gacccctaac cctttaagca
    12661 atgataccga agccgcgctg atcgccgaga tccgcacgtc cggttggctg tcagcgacaa
    12721 acggtgcagg ttctgcgtgc tcgatcaccg ttaccgaaaa tccgtgctgt gccagcccca
    12781 gcgccagtgc gccgccgacc attcctccgc cgacaatggc aatttccgtt ggttgatttg
    12841 tcatggtcaa tcatcctgtt agcaatatgc tttaagttta caggattttt acccccaagg
    12901 gctgataacg ctcatactgg tcacaacggc agcaaagcat tacactatgc gccctgcatt
    12961 cctggctact atttcgcaag agcaagtcga tgaccaaaaa actccatatt aaaacctggg
    13021 gctgtcagat gaacgagtac gattcatcga agatggccga tctgctggat gccacccacg
    13081 gctatcaact gaccgacgtg gcggaagaag cggatgtgct actgctgaac acctgctcaa
    13141 tccgcgagaa ggctcaggaa aaagtcttcc atcagttggg tcgctggaaa ctgttaaaag
    13201 agaagaatcc agacctgatt atcggcgtcg gtggctgcgt ggcatcgcaa gaaggcgagc
    13261 acattcgcca gcgcgcccac tatgtcgata ttatttttgg gccgcaaacg ctgcaccgtc
    13321 tgccggagat gatcaactcc gtgcgcggcg accgcagccc ggttgtagat atcagcttcc
    13381 cggaaatcga gaagtttgac cgtctgccgg aaccgcgcgc cgaagggccg accgcgtttg
    13441 tctccatcat ggaaggctgc aataaatatt gcacctactg cgtggtgcct tacacccgtg
    13501 gtgaagaggt aagccgtccg tctgacgata ttctgtttga gattgcccag cttgcggctc
    13561 agggcgtgcg tgaagtcaac ctgctcggtc agaatgtgaa tgcctggcgt ggtgagaact
    13621 acgacggcac caccggatcg tttgccgatc tgttgcgtct ggttgctgcg atcgacggga
    13681 tcgatcgtat tcgctttacc accagccatc cgatcgaatt caccgacgat atcatcgaag
    13741 tgtaccgcga cacgcctgag ctggtgagct tcctgcatct gccggtacag agcggttccg
    13801 atcgcattct gaacctgatg ggacgtaccc atacggcgct ggagtacaaa gcgatcatcc
    13861 gtaaactgcg tgcggcgcgt ccggatattc agatcagttc tgacttcatt gttggcttcc
    13921 ctggcgaaac caccgaagac ttcgagaaaa cgatgaagct gattgccgac gtcaatttcg
    13981 acatgagcta cagctttatc ttctctgcac gtccaggaac gcctgccgcc gatatggttg
    14041 atgatgttcc ggaagaagag aagaagcagc gtctgtatat tctgcaagaa cgtatcaatc
    14101 agcaagcgat ggcatggagc cgtcggatgc tcggcaccac ccagcgtatt ctggtagaag
    14161 ggacttcgcg taagagcatc atggagcttt ccgggcgtac cgaaaataac cgcgtggtca
    14221 acttcgaggg cacaccggat atgatcggta aattcgtcga tgtagaaatt accgacgtct
    14281 acccgaactc tctgcgcggt aaagtggtgc gaactgaaga tgaaatgggt ctgcgcatgg
    14341 cagaaacacc ggagtcggtg attgcccgca cccgcaaaga aaacgacctt ggcgtgggtt
    14401 attatcagcc gtaattctca ggcctgccgt tccggtgggc cttgtttttt ctctctttat
    14461 ccccatcttt atcgcaatgc ttgcgccttt ttcctgtggc gtgaataatt tccttatagg
    14521 ccggtgaatg ttcagcttcc ggcggcatac agataagagg aacggtttga acatagacac
    14581 tcgcgaaatc accctggagc cagcagacaa tgcgcgtctg ttgagcctgt gcggcccgtt
    14641 tgatgacaac atcaagcagc tcgaacgccg tctcggcatc gagatcaatc gccgcgataa
    14701 ccactttaaa ctgaccggcc gtccgatttg cgtcaccgct gcggcagaca ttctgcgtag
    14761 cctgtatgtc gatactgccc cgatgcgcgg tcagattcag gatatcgaac cggaacagat
    14821 ccaccttgcg attaaagaag cgcgggtact ggagcaaagc gcggagagcg tgccggagta
    14881 cggcaaagcg gtcaatatca aaactaaacg cggcgtaatt aagccgcgca cgccaaacca
    14941 ggcgcagtac atcgccaata ttctcgacca tgacattacc ttcggcgttg gcccggcggg
    15001 tacgggtaaa acctacctgg cagtggctgc ggcagttgat gccctggagc gtcaggaaat
    15061 tcgccgtatt ctgctgactc gtccggcggt cgaagccggt gagaaactgg gcttcctgcc
    15121 tggcgattta agccagaaag tagacccgta tctgcgccca ctgtacgacg cgctgtttga
    15181 aatgctgggc tttgagaaag tcgagaaact gattgagcgc aacgttattg aagtcgcgcc
    15241 gctggcctat atgcgtggtc gtacgctgaa cgacgcgttt atcattctcg atgagagcca
    15301 gaacactacc atcgaacaga tgaagatgtt cctgacccgt atcggtttta actcaaaagc
    15361 ggttatcacc ggcgacgtca cacagatcga cctgccgcgt aatactaaat caggcttacg
    15421 tcatgccatt gaagtgctgg ccgatgtcga agagatcagc tttaacttct tccacagcga
    15481 agacgtggtt cgtcacccgg tggtggcgcg tatcgttaac gcttatgaag cctgggaaga
    15541 agcagaacaa aaacgtaaag cggcgctggc agcagaacgc aagcgcgaag aacaggaaca
    15601 aaaatgagtc aggtgatcct cgatttacaa ctggcatgtg aagataattc cgggttaccg
    15661 gaagagagcc agtttcaaac atggctgaat gcggtgatcc cgcagtttca ggaagagtcg
    15721 gaagtgacta ttcgcgtggt cgataccgcc gaaagccaca gtctgaatct gacctatcgc
    15781 ggtaaggata agccgaccaa cgtgctctcc ttcccgtttg aagtgccgcc tggcatggag
    15841 atgtcgctac tgggcgatct ggttatctgc cgtcaggtgg ttgagaagga agctcaggag
    15901 caaggcaaac cactggaggc gcactgggcg catatggtgg tgcacggcag tctgcatttg
    15961 ttaggttacg atcacattga agatgacgaa gcagaagaaa tggaagccct cgaaacagag
    16021 attatgcttg ctctgggcta tgaggatccg tacattgccg agaaagaata atttgccagg
    16081 catttgactg gcagacgtcc ccgccgttgg tgattaacga cggcgttttg attaactaac
    16141 atgagaaccc atacgacgcc atgagcgacg acaattcaca cagtagtgac acgataagca
    16201 acaagaaggg atttttctcc ctgttactca gccaactttt ccacggtgaa ccgaaaaacc
    16261 gtgacgaact gctggcgctg atccgtgatt ccgggcagaa cgaccttatc gacgaagata
    16321 cgcgcgatat gctcgaaggg gtgatggaca tcgcagacca acgcgttcgc gacatcatga
    16381 tcccccgctc ccagatgatt accctgaaac gcaaccagac gctggacgaa tgccttgatg
    16441 tcatcatcga gtccgcccac tcacgtttcc cggtgattag cgaagacaaa gatcacattg
    16501 aagggattct gatggcgaaa gacttgctgc cgtttatgcg cagcgatgct gaagccttca
    16561 gcatggacaa agtgttacgt caggcggttg tcgttcctga aagtaagcgc gtagaccgga
    16621 tgctgaaaga gtttcgctct cagcgttacc acatggcgat cgttattgac gaattcggtg
    16681 gggtttccgg tctggtgacc attgaagaca tcctggaact gattgttggt gagattgaag
    16741 acgagtatga cgaagaagat gatatcgact tccgtcagct gagtcgtcat acctggaccg
    16801 tgcgcgcact ggcttccatt gaagacttca acgaagcgtt cggcacccac tttagcgatg
    16861 aagaagtcga cactatcggt ggtctggtga tgcaggcgtt tgggcatctt ccggcgcgtg
    16921 gcgaaactat cgacatcgac ggttaccagt tcaaagtggc gatggccgat agtcggcgta
    16981 ttattcaggt tcatgtcaaa atcccggatg actcacccca gccaaagctg gatgaataaa
    17041 accgaaactg gatagataac tacatggatt ttgcctcatt aattgaacgc cagcgcattc
    17101 gcctgctgct ggcgttatta ttcggtgcct gcggaacgct ggccttctct ccttacgacg
    17161 tctggcctgc ggcgattatt tcgctgatgg ggcttcaggc gttgaccttt aaccgtcgcc
    17221 cactccagtc tgccgctatt ggcttttgct ggggatttgg cctctttggc agcggtatta
    17281 actgggtcta tgtcagcatc gcgacctttg gcgggatgcc tggcccggtt aacatcttcc
    17341 tggtggtgct gctggcggcg tatttgtcgc tgtataccgg actgtttgct ggcgtgctgt
    17401 cgcgtctgtg gccgaaaacc acctggctgc gcgtggcgat tgccacccct gctctctggc
    17461 aagtgaccga gtttctgcgc ggttgggtgc tgactggctt cccgtggtta cagttcggct
    17521 atagccagat tgatggcccg ttaaaagggc tggcaccgct aatgggcgtg gaagccatta
    17581 acttcctgct gatgatggtt agtggcctgc tggcactggc gttggtcaaa cgcaactggc
    17641 gtccgctggt ggtggccgtc gtgctgtttg ccctaccctt cccgctgcgt tacatccaat
    17701 ggtttacccc gcaaccggag aaaaccattc aggtttcgat ggttcagggc gatattccgc
    17761 aatcgctgaa atgggacgaa ggccagcttc ttaatacgct aaagatttac tacaacgcaa
    17821 cggcaccgct gatgggcaaa tcatcgttga ttatctggcc ggagtcggcg ataaccgatc
    17881 tggaaattaa tcagcaaccg ttcctcaaag cactggacgg tgagttgcgt gataaaggta
    17941 gctcgctggt aaccgggatt gtcgacgcgc gtctcaataa gcagaaccgc tacgatacct
    18001 acaacaccat catcacgctg ggtaaaggtg caccgtacag ctacgaatca gccgatcgct
    18061 ataacaaaaa ccatctggtg ccgtttggcg agtttgtccc gctggagtcg attctgcgtc
    18121 cgttagcacc gttctttgat ctgccgatgt cgtcgttcag ccgtgggcca tatatccagc
    18181 cgccgctgtc ggcaaatggt attgagctta ctgcggctat ttgctacgag attattctcg
    18241 ccgagcaagt gcgcgataac ttccgcccgg ataccgacta cctgctgact atctccaacg
    18301 atgcgtggtt tggtaaatct attggtccat ggcaacactt ccagatggcg cgaatgcgtg
    18361 cgctggagct ggcgcgccca ctgttgcgca gcaccaacaa cggcattacg gcggtgattg
    18421 gcccgcaggg tgagattcag gcgatgatcc cgcagttcgc ccgcgaggtg ttaaccacta
    18481 acgtgacgcc gaccaccgga ctcacaccat acgcacgtac cggcaactgg ccgctgtggg
    18541 tgctgacggc actgtttggt tttgctgctg tgttgatgag tctgcgtcag cgacgtaaat
    18601 aatcccttct tatcgtgccg gaatacatcc catatttcgg cacgctcacc ggatgcggca
    18661 tcagtgcatt atccggataa caatattctc ctatccaact ttggcacatc tattgctttg
    18721 ttatacaagg caaagcttga accagcatca gtacaacccg gttgcaccac agcaatccac
    18781 cggtagcacc aaaagagtgc attttaagaa ttttgcttca gaatggtgcg gtgcacctcg
    18841 caaaaacaaa caataaaaca tcaatatctt tacatttagc caaactaaat gttacctaac
    18901 aggcacaaca ctgcacaata aagttgcaga cgataacaac acaaacactc acaacgggta
    18961 tccatgcgtt cttaacgcag aagataaagg tgttggatat gcaattacgt aaacctgcca
    19021 cagcaatcct cgccctggcg ctttccgcag gactggcaca ggcagatgac gccgccccgg
    19081 cagcaggcag tacgctggac aaaatcgcca aaaacggtgt gattgtcgtc ggtcaccgtg
    19141 aatcttcagt acctttctct tattacgaca atcagcaaaa agtggtgggt tactcgcagg
    19201 attactccaa cgccattgtt gaagcagtga aaaagaaact caacaaaccg gacttgcagg
    19261 taaaactgat tccgattacc tcacaaaatc gtattccact gctgcaaaac ggcactttcg
    19321 attttgaatg tggttctacc accaacaacg tcgaacgcca aaaacaggcg gctttctctg
    19381 acactatttt cgtggtcggt acgcgcctgt tgaccaaaaa gggcggcgat atcaaagatt
    19441 ttgccgacct gaaaggcaaa gccgtagtcg tcacttccgg cactacctct gaagttttgc
    19501 tcaacaaact gaatgaagag caaaaaatga atatgcgcat catcagcgcc aaagatcacg
    19561 gtgactcttt ccgcaccctg gaaagcggtc gtgccgttgc ctttatgatg gatgacgctc
    19621 tgctggccgg tgaacgtgcg aaagcgaaga aaccagacaa ctgggatatc gtcggcaagc
    19681 cgcagtctca ggaagcctac ggttgcatgt tgcgtaaaga cgatccgcag ttcaaaaagc
    19741 tgatggatga cactatcgct caggtgcaga cctccggtga agcggaaaaa tggtttgata
    19801 aatggttcaa aaatccaatt ccgccgaaaa acctgaacat gaatttcgaa ctgtcagacg
    19861 aaatgaaagc actgttcaaa gaaccgaatg acaaggcact gaactaatta caagaaccag
    19921 gggcggaaat tccagccctc tcgattgtta cgtagcacgg acagactata cgcctgatgg
    19981 tcgttcccca tcgggcctga aaaccgcaat acgctgggta acaatcttcg agggtagcag
    20041 ttaacgctgc taccctcttt tttctggagt agatttatgt ctatagactg gaactggggt
    20101 atttttttac aacaagcccc gttcggcaat accacctatc ttggttgggt ctggagtggt
    20161 tttcaggtga cgatcgcttt gtcgatctgc gcctggatta tcgctttcct cgtcggttca
    20221 tttttcggca ttttacgtac cgttccaaac cgttttctct ccggtctggg tacgttgtat
    20281 gtcgaactgt tccgcaacgt gccgttgatt gtgcaattct ttacctggta tctggtgatc
    20341 ccggagctgc tgccggagaa aatcggcatg tggtttaagg ccgagctgga tcccaatatt
    20401 cagtttttcc tttcatccat gctctgcctg gggctgttta ccgccgcccg tgtttgcgaa
    20461 caggtacgcg ccgctattca gtcgctaccg cgtgggcaga agaatgccgc gctggcgatg
    20521 ggcctgacgc taccgcagac ttatcgatac gtactgctgc ctaatgctta tcgcgttatc
    20581 gtcccgccga tgacttccga gatgatgaac ctggtgaaaa actccgccat cgcctcgacc
    20641 atcggtctgg tggatatggc ggcgcaggcg ggtaaattgc tcgactactc ggctcacgcc
    20701 tgggagtcgt ttaccgccat cacgcttgcg tatgtgttga ttaacgcttt tatcatgctg
    20761 gtgatgacgc tggttgaacg taaagttcgc ctgcccggca acatgggggg caaataatgt
    20821 acgagtttga ctggagttcc attgttcctt ccctgccata tctgctcgac gggctggtga
    20881 tcaccctgaa aatcaccgtc acggcagtcg tgattggtat tttgtggggt accatgctgg
    20941 cggtgatgcg cttgtccagc ttcgcgcctg tttcgtggtt tgccaaagcc tacgttaacg
    21001 tattccgctc gattccttta gtcatggttt tgctgtggtt ttacttgatt gtgccgggat
    21061 ttctgcaaaa tgtgctggga ttatcgccaa aaaatgatat tcgtttaatt tcggcgatgg
    21121 tggcgttttc gatgtttgaa gcggcctact attcagagat tatccgtgcc ggtattcaaa
    21181 gtatttcccg tggtcagtcg agcgccgcgc tggcgttggg gatgactcac tggcagtcga
    21241 tgaaactgat tattctgccg caggctttcc gcgcgatggt gccgctgctg ctcactcagg
    21301 gcatcgtact gttccaggat acctcgctgg tgtatgtgtt atctctggcc gatttcttcc
    21361 gtaccgcctc aaccattggt gagcgtgatg gtacgcaggt cgagatgatc ctgtttgccg
    21421 gatttgttta tttcgttatt agccttagcg cgtcgttgtt ggtcagctac ttgaaaagaa
    21481 ggacagcata atgattaccc tgaaaaatgt ttcaaaatgg tatggtcact ttcaggtgct
    21541 gaccgactgc tcaaccgaag tgaaaaaagg cgaagtggtg gtggtttgcg gcccgtctgg
    21601 ttccggcaaa tcaacgctga ttaaaaccgt caacggcctc gaaccagtgc agcaaggtga
    21661 aatcaccgtc gatggtatcg tggttaacga caagaaaacc gatctggcaa agctgcgctc
    21721 ccgcgtcggg atggtattcc agcatttcga gctgttccct catctgtcga ttatcgaaaa
    21781 cctgaccctg gcgcaggtga aagtgcttaa acgcgataaa gcgcccgctc gtgaaaaagc
    21841 attgaaattg ctggagcgcg tgggactttc cgcacatgcc aataaatttc cggcgcagct
    21901 ttccggcggt cagcagcagc gcgtggcaat cgctcgcgca ttgtgtatgg ctcctattgc
    21961 gatgctgttc gacgaaccga catcggcgct ggatccggag atgatcaacg aagtgctgga
    22021 cgtgatggtg gaactggcga acgaaggaat gaccatgatg gtggtgaccc acgaaatggg
    22081 ctttgcccgc aaagtggcga atcgggtgat ctttatggac gagggtaaaa ttgtcgaaga
    22141 ctcgccgaaa gacgctttct tcgatgatcc gaaatcggac cgcgcaaaag acttcctcgc
    22201 gaaaatcctg cattaatcac tctggcgcac atacccaatt gcgcgccatc tcacgcttta
    22261 tcgtttcgcc cctcgtatct gttacatcct ggcgttaccc ttgtcgcaaa gaagcacaca
    22321 acaaggagca acaatggcac tgccaattct gttagattgc gacccaggtc atgatgacgc
    22381 tatcgcaata gttctcgcac tcgcctcacc agagcttgat gtcaaagcaa ttacgtcttc
    22441 cgccggaaac cagacaccag aaaaaacctt acgcaatgtt ctgcgtatgc tgaccttgct
    22501 taatcgcacc gatattccgg tggcaggcgg cgcggtaaaa ccgttaatgc gtgagttgat
    22561 tatcgcggac aatgtgcacg gcgaaagcgg tctcgacggc ccggcattac cggaaccgac
    22621 gttcgcaccg caaaactgta cggcggtgga gctgatggcg aaaacgctgc gcgaaagtgc
    22681 ggaacctgtc acgattgttt ctaccggacc gcaaactaac gttgccttgc tgctcaatag
    22741 ccatccggaa ctgcatagca aaattgcccg tatcgtgatt atgggcggcg caatggggct
    22801 tggtaactgg acgcctgcgg ctgaatttaa tatttacgtt gacccggaag cggcagaaat
    22861 tgtcttccag tcagggatcc cggtggtgat ggccggtctg gatgttactc ataaagcgca
    22921 aatccacgtt gaagacaccg agcgtttccg cgcgattggc aaccctgttt caaccattgt
    22981 tgccgaactg ctggatttct tcctcgaata tcataaagac gaaaaatggg gctttgtcgg
    23041 cgcaccgctg catgacccat gcaccatcgc ctggctgttg aaacccgagt tgtttaccac
    23101 tgttgagcgc tgggttggcg tggaaacaca ggggaaatat acccagggta tgacggttgt
    23161 tgattattat tatctgactg gcaataaacc gaatgccact gtaattgtcg atgttgatcg
    23221 tcagggcttt gttgatttac tggctgatcg tctgaaattt tacgcttaac actcgtcatt
    23281 gattaagatc accacgcgtt acctgggtgg tcttaatcta tttatctctt tttctttcaa
    23341 gatataaatg caatggataa tgcagaactc gccataggta tcgatctcgg cactaccaat
    23401 agtttaattg ccgtctggaa agacggtgcc gcgcaattaa ttccaaataa gttcggtgaa
    23461 tatttaacac catccataat tagcatggat gaaaataatc atattttagt cggaaaaccg
    23521 gctgtatcac ggcgtacttc gcatccggat aaaacggcag cgttatttaa acgtgcaatg
    23581 ggcagtaata ccaactggcg gttaggcagc gacacattta acgcgccaga actgtcctct
    23641 ttggtattac gctcattaaa agaagatgcc gaagaatttc tgcaacgtcc gattaaagat
    23701 gtggtgatct ccgttctggc ttatttcagc gatgaacaac gcaagcatac ccgtttagca
    23761 gcggacttag ccgggttaaa tgcggtacgc ttaattaatg aacccacagc agctgcgatg
    23821 gcgtatggcc tgcataccca acaaaatacc cgttcgctgg tgtttgatct cggtggcggc
    23881 acgtttgacg ttacggtgct tgagtacgcc acgccggtga ttgaagttca cgcctccgct
    23941 ggcgacaact tcctcggtgg cgaagatttt acccatatgt tggtcgatga cgttttaaag
    24001 cgcgcggatg tcacaaggac cacgcttaac gagagtgaac tggcagcctt gtacgcctgt
    24061 gtggaagcgg caaaatgtag caatcaatcg ccaatgcaca ttcgctggta gtaccaggat
    24121 gaaacgcggg aatgcgaatt ttacgagaac gaactggaag atttgtggtt gccgctgctc
    24181 aatcgcttgc gagtgccgat tgaacaggcg ttgcgcgatg cgcgtctgaa gccgagtcaa
    24241 atcgacagtc tggtgctggt tggcggcgcg tcacaaatgc cgctggtgca gcgaatcgcc
    24301 gtgcgtctgt ttggcaaatt accgtatcaa agttacgaac cgagcaccat tgtcgcgctg
    24361 ggcgcagcaa tccaggccgc ctgccgctta cgcagtgaag atattgaaga agtgatcctc
    24421 actgatattt gcccttactc gttgggcgtt gaggttaacc gccagggcgt ttccggcatt
    24481 ttctcgccga ttattgaacg aaacaccact gtgcccgtgt cgcgtgtaga aacttattca
    24541 accatgcacc cggaacagga ttcaattacg gttaacgtct atcagggaga aaaccacaaa
    24601 gttaaaaaca acattctggt ggaatccttc gatgtgccgt tgaagaaaac cggggcttat
    24661 cagtcgattg atattcgctt tagttatgat atcaacgggt tgcttgaagt tgacgtgctt
    24721 ctggaagacg gcagcgttaa gtccagagtg attaaccaca gcccggtaac attgagcgcg
    24781 cagcagattg aagagagtcg gacgcggtta tccgcattga aaatttatcc gcgcgatatg
    24841 ctcatcaatc gcacctttaa agccaaactg gaagagttgt gggcgcgggc gctgggtgac
    24901 gagcgagaag agattggccg ggtgatcacc gattttgatg cggcgctgca gtcaaacgat
    24961 atggcccgcg tcgatgaggt caggcggcgg gcgagcgttt atttagccat tgagacctca
    25021 taatcacggc tgccggatgc ggcgtaaatg ctgcatccgg catcggcgca aaaaacacat
    25081 cgtcaactaa acaacatcgc gattttgccg agagcataac ctataaccag caccgttaaa
    25141 ctgattttga taatgctggt aatgctctgt ggtttttcac cctcatcatt tttttcttca
    25201 tggttttcag gtgttggctc atcgacatct tcatcctccg gctcgagttt tactttcgtc
    25261 aacagcatcc gggtgataat ggcctctaat ggatgagcat ctccgccttc cagccttgta
    25321 ttaagacggg ccagcatctc ttgtttcgga aacgcttcaa gtactacctg ttccgctacc
    25381 cgatacaaat ccagtaacgg tgaatcttct tctgtctcgc taccgactct cgatttccac
    25441 cacgcccatg ctttgcgttt gaagaataac gactcaataa acgattgacc taacggcgat
    25501 aattttccgc tatgtaaaat ttccgcccat aacagccgcg tccgggcgct ctcaccagga
    25561 acatatttca gcggttgacc ttgcgcatct accatgctcc gcgcttcgat aaccatcact
    25621 aataacgcga aataatccgg gcaatgacgc ttgctccact gcaacaacaa attatcagct
    25681 tgtgtagatg ggtaactttc ccagtacgcg ccgagatcag caagcaggct ttccccctcg
    25741 ccgcagtaca aacgttgtgc acactggtaa taccgggcat cttcgctttc tggttcttcc
    25801 gcttgccact gtttggctac aggaatgagt tccaccatac ctgtttgcac cgagctgtac
    25861 cagcgcaaca gtttgcgatg taactttgca tcatcaggaa tgaaccacgg tccttccatc
    25921 gccaaaaacg cagtgacata gtcaggatgg taacggcagc aacgttccag cgcgaagtaa
    25981 tattcaacag tctggttctg taacgcgacg ggatattcca gaaggctaag gaaatcgaaa
    26041 caatcaccag cctgaatggc ctcaagaaaa gcctcccttt cttcctcatc cattccttcg
    26101 ctgtcattaa acgaatgcca gttcaaacgc tctgccaaca aagaagcaca atttaatgac
    26161 agatatcgcg cttctatagc gattgcacac agcggccagc gtaattgatc gacatcctcc
    26221 atgttccagg tatttaattg ctgaataaat ttctgccagg cggagggctg aaaacgatca
    26281 ctttctgaat ccagtaatgt cctgaatgca cgtagtattt catgttcagc ggtggcatct
    26341 ttttcttcat catctgcttc ctcgacagga tttaccgcca gtcgcagcgc ctcttcatag
    26401 gcctggcgta atgctttaaa cccctgcgga tcggtttcgg gatgacacaa cggtaagcga
    26461 gcaagataag cctggcggat aatgtctatt tgcgtcgtgc tttcaatttc gaggatttgc
    26521 caacatgttt tcataggtcg ctcacatcct taagcgttac cgggcattca ggctggacag
    26581 ttaatggctg ccagggaagc gtggcataga aacggtcgct tcccgctgcc agctcttcga
    26641 acatccgact cccggcgcta tcgtatttgt acagggttag cgcctcgcgt agcgaagcgt
    26701 tgtcttcgca ctgagaagat tgccagtaga gacgccctag tgaataggca gaaaaatagt
    26761 gcatccagct gtgatagtaa tggtgagctc gtgcatagac gcgcgattgc agccataaac
    26821 tctcttcttc cgtcagccag ttgttgagca caccggcccg tgacagaaat cccattcgaa
    26881 cataatccca ggctttgatt cccccttctc cacagcacat agcggtttgg gcaacgaatt
    26941 gggtataaat caaccctctc tccgagccac cagcaattaa tgctttccac tcttcagggg
    27001 aagagcgttg ccatgtcaga tataagccag ctaaatcatt ggcatgacca tcatcagtca
    27061 tctgataaat agtctgaatt aaatcatcac gattatttat tccccaggat gatgagagat
    27121 cgaattcttt tatcccggta tacatttttg gcgagtcatg cgcgggtttg caactctgat
    27181 taagtatttc catgggcgca gaaagcgcaa aaagaagata ttgttcttcc ttattcattt
    27241 ttt
//
LOCUS       DAZPCS010000039        26186 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZPCS010000039 DAZPCS010000000
VERSION     DAZPCS010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26186
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.039"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..215)
                     /locus_tag="ACHUQ7_001652"
     CDS             complement(<1..215)
                     /locus_tag="ACHUQ7_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HFR1135104.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDA"
     gene            525..2297
                     /gene="aspS"
                     /locus_tag="ACHUQ7_001653"
     CDS             525..2297
                     /gene="aspS"
                     /locus_tag="ACHUQ7_001653"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFR1135105.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALLLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2415..2867
                     /gene="nudB"
                     /locus_tag="ACHUQ7_001654"
     CDS             2415..2867
                     /gene="nudB"
                     /locus_tag="ACHUQ7_001654"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFR1135106.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            2896..3636
                     /locus_tag="ACHUQ7_001655"
     CDS             2896..3636
                     /locus_tag="ACHUQ7_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR1135107.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            3671..4192
                     /gene="ruvC"
                     /locus_tag="ACHUQ7_001656"
     CDS             3671..4192
                     /gene="ruvC"
                     /locus_tag="ACHUQ7_001656"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFR1135108.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLTRGRLR"
     gene            complement(4194..4796)
                     /locus_tag="ACHUQ7_001657"
     CDS             complement(4194..4796)
                     /locus_tag="ACHUQ7_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFR1135109.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            4866..4931
                     /gene="yobI"
                     /locus_tag="ACHUQ7_001658"
     CDS             4866..4931
                     /gene="yobI"
                     /locus_tag="ACHUQ7_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HFR1135110.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            5070..5681
                     /gene="ruvA"
                     /locus_tag="ACHUQ7_001659"
     CDS             5070..5681
                     /gene="ruvA"
                     /locus_tag="ACHUQ7_001659"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFR1135111.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDTSSETLIREALRAAL"
     gene            5690..6700
                     /gene="ruvB"
                     /locus_tag="ACHUQ7_001660"
     CDS             5690..6700
                     /gene="ruvB"
                     /locus_tag="ACHUQ7_001660"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFR1135112.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGAQEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(6847..7632)
                     /gene="znuB"
                     /locus_tag="ACHUQ7_001661"
     CDS             complement(6847..7632)
                     /gene="znuB"
                     /locus_tag="ACHUQ7_001661"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFR1135113.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(7629..8384)
                     /gene="znuC"
                     /locus_tag="ACHUQ7_001662"
     CDS             complement(7629..8384)
                     /gene="znuC"
                     /locus_tag="ACHUQ7_001662"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFR1135114.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            8463..9395
                     /gene="znuA"
                     /locus_tag="ACHUQ7_001663"
     CDS             8463..9395
                     /gene="znuA"
                     /locus_tag="ACHUQ7_001663"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFR1135115.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            9411..10733
                     /gene="mepM"
                     /locus_tag="ACHUQ7_001664"
     CDS             9411..10733
                     /gene="mepM"
                     /locus_tag="ACHUQ7_001664"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFR1135116.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            10853..11824
                     /gene="lpxM"
                     /locus_tag="ACHUQ7_001665"
                     /gene_synonym="msbB"
     CDS             10853..11824
                     /gene="lpxM"
                     /locus_tag="ACHUQ7_001665"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFR1135117.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(11955..13397)
                     /gene="pyk"
                     /locus_tag="ACHUQ7_001666"
     CDS             complement(11955..13397)
                     /gene="pyk"
                     /locus_tag="ACHUQ7_001666"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFR1135118.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPTDVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVPFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(13525..14394)
                     /gene="hexR"
                     /locus_tag="ACHUQ7_001667"
                     /gene_synonym="yebK"
     CDS             complement(13525..14394)
                     /gene="hexR"
                     /locus_tag="ACHUQ7_001667"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFR1135119.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            14731..16206
                     /gene="zwf"
                     /locus_tag="ACHUQ7_001668"
     CDS             14731..16206
                     /gene="zwf"
                     /locus_tag="ACHUQ7_001668"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFR1135120.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKVGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            16441..18252
                     /gene="edd"
                     /locus_tag="ACHUQ7_001669"
     CDS             16441..18252
                     /gene="edd"
                     /locus_tag="ACHUQ7_001669"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFR1135121.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPENKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            18289..18930
                     /gene="kdgA"
                     /locus_tag="ACHUQ7_001670"
                     /gene_synonym="eda"
     CDS             18289..18930
                     /gene="kdgA"
                     /locus_tag="ACHUQ7_001670"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFR1135122.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(18986..20164)
                     /gene="purT"
                     /locus_tag="ACHUQ7_001671"
     CDS             complement(18986..20164)
                     /gene="purT"
                     /locus_tag="ACHUQ7_001671"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFR1135123.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSKSLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRHLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            20298..20588
                     /gene="yebG"
                     /locus_tag="ACHUQ7_001672"
                     /pseudo
     CDS             20298..20588
                     /gene="yebG"
                     /locus_tag="ACHUQ7_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            20655..21011
                     /gene="yebF"
                     /locus_tag="ACHUQ7_001673"
     CDS             20655..21011
                     /gene="yebF"
                     /locus_tag="ACHUQ7_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFR1135124.1"
                     /translation="MKKRGAFLGRLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVCVDCKAGMAEYQRR"
     gene            21338..21997
                     /locus_tag="ACHUQ7_001674"
     CDS             21338..21997
                     /locus_tag="ACHUQ7_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFR1135125.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVSGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            22207..24267
                     /gene="ptrB"
                     /locus_tag="ACHUQ7_001675"
     CDS             22207..24267
                     /gene="ptrB"
                     /locus_tag="ACHUQ7_001675"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFR1135126.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPKVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(24264..24926)
                     /gene="exoX"
                     /locus_tag="ACHUQ7_001676"
     CDS             complement(24264..24926)
                     /gene="exoX"
                     /locus_tag="ACHUQ7_001676"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFR1135127.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPLGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERYPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(24950..25606)
                     /gene="yobB"
                     /locus_tag="ACHUQ7_001677"
                     /pseudo
     CDS             complement(24950..25606)
                     /gene="yobB"
                     /locus_tag="ACHUQ7_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
     gene            complement(25708..25938)
                     /gene="holE"
                     /locus_tag="ACHUQ7_001678"
     CDS             complement(25708..25938)
                     /gene="holE"
                     /locus_tag="ACHUQ7_001678"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFR1135128.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            26077..>26186
                     /locus_tag="ACHUQ7_001679"
     CDS             26077..>26186
                     /locus_tag="ACHUQ7_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1135129.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAA"
BASE COUNT         6652 a         6486 c         6819 g         6229 t
ORIGIN      
        1 gcatcaaccg gctgttttaa tgcttcggcg taatcggcag accagccaac tcgcaccaga
       61 aacacgggct gaccgctggc gcgaaatttc gccgccagct tcccggcgcg attaactacc
      121 tcatcggcag tatgtggacc accggcaaaa ggtaagatgc cttcttgtaa atcgatcacc
      181 accaacgcag tggttttagc attaagttca agcatgctga ctcctgaaaa aacaaaatcg
      241 tgcctcacac cttaatgcga ttagccgatg gcggaacgac aaattttgtt aatttttatg
      301 agttcgggca acacctttat agcaaactgg cgtagagtct gatttcaggc ccgatataaa
      361 gtggtaacga ggcgttccag cgcgggccag gctcttacgg tgcggcagaa tttccagtat
      421 aatagccgcc ttttttcatt cagttgtgac atacagctaa cgctgcgact tggtcacctg
      481 cgcagcaggg ggcatccgcc ggcagctaag ttaagggata tctcatgcgt acagaatatt
      541 gtggacagct ccgtttgtcc cacgtggggc agcaggtgac tctgtgtggt tgggtcaacc
      601 gtcgtcgtga tcttggtagc ctgatcttca tcgatatgcg cgaccgcgaa ggtatcgtgc
      661 aggtattttt cgatccggat cgtgcggacg cgttaaagct ggcctctgaa ctgcgtaatg
      721 agttctgcat tcaggttacg ggcaccgtac gtgcgcgtga cgaaaaaaat attaaccgcg
      781 atatggcgac cggcgaaatt gaagtgctgg cgtcctcgct gactatcatc aaccgcgcag
      841 atgttctgcc gcttgactct aaccacgtca acaccgaaga agcgcgtctg aaataccgct
      901 acctcgacct gcgtcgcccg gaaatggcgc aacgcctgaa aacccgcgct aaaatcacca
      961 gcctggtgcg ccgttttatg gatgaccacg gcttcctcga catcgaaact ccgatgctga
     1021 ccaaagccac gccggaaggc gcgcgtgact acctggtgcc ttctcgtgtg cacaaaggta
     1081 aattctacgc actgccgcaa tccccacagt tgttcaaaca gctgctgatg atgtccggtt
     1141 ttgaccgtta ctatcagatc gttaaatgct tccgtgacga agacctgcgc gctgatcgtc
     1201 agcctgaatt tactcagatc gatgtggaaa cttctttcat gaccgcgccg caagtgcgtg
     1261 aagtgatgga agcgctggtg cgtcatttgt ggctggaagt gaagggcgtg gatctgggcg
     1321 atttcccggt aatgaccttt gcggaagcag aacgccgtta tggttctgat aaaccggatc
     1381 tgcgtaaccc gatggaactg accgacgttg ccgatctgct gaaatctgtt gagtttgcag
     1441 tatttgcggg gccagcgaac gatccgaaag gccgtgttgc cgctctacgt gttccgggtg
     1501 gcgcatcgct gacccgtaag cagatcgacg aatacggtaa cttcgttaaa atctacggcg
     1561 cgaaaggtct ggcttacatc aaagttaacg aacgcgcgaa aggtctggaa ggtatcaaca
     1621 gcccggtagc gaagttcctt aatgcagaaa tcatcgaagc catcctggat cgtactgccg
     1681 cgcaagatgg cgatatgatt ttcttcggtg ccgacaacaa gaaaattgtt gccgacggga
     1741 tgggcgcact gctcctgaaa gtgggtaaag accttggtct gaccgacgaa agcaaatggg
     1801 caccgctgtg ggttatcgac ttcccgatgt ttgaagacga cggtgaaggc ggcctgacgg
     1861 caatgcacca tccgttcacc tcaccgaaag atatgactgc tgcagaactg aaagctgcac
     1921 cggaaaatgc ggtggcgaac gcttacgata tggtcatcaa tggttacgaa gtgggcggtg
     1981 gttcagtacg tatccataat ggtgatatgc agcagacggt gtttggtatt ctgggtatca
     2041 acgaagagga acagcgcgag aaattcggct tcctgctcga cgccctgaaa tacggtactc
     2101 cgccgcacgc aggtctggca ttcggtcttg accgtctgac catgctgctg actggcaccg
     2161 acaatatccg tgacgttatc gccttcccga aaaccacggc ggcagcgtgt ctgatgactg
     2221 aagcaccgag ctttgctaac ccgactgcac tggctgagct gagcattcag gttgtgaaga
     2281 aggctgagaa taactgatat gactcaaata cacgaaatca ttcgcgttgc atcaaggcgg
     2341 caactgagtg aactcccatg agcatagata actatgtgaa tgggatgagc gaaggcagtc
     2401 aacgaagagg cagcgtgaag gataaagtgt ataagcgtcc cgtttcgatc ttagtggtca
     2461 tctatgcaca agatacgaaa cgggtgctga tgttgcagcg gcgtgacgat cccgatttct
     2521 ggcagtcggt aaccggcagc gtggaagagg gtgaaaccgc gccgcaagct gccatgcgcg
     2581 aagtaaagga agaggttacc attgatgttg tcgctgaaca actgacctta attgactgtc
     2641 agcgcacggt agagtttgaa attttttcac atttacgtca tcgctatgcg ccgggcgtga
     2701 cgcgtaatac ggaatcatgg ttctgtcttg cgcttccgca cgagcggcag atcgttttca
     2761 ctgaacatct ggcttacaag tggcttgatg cgcctgctgc ggcggcgctc actaagtcct
     2821 ggagcaaccg gcaggcgatt gaacagtttg taattaacgc tgcctgaaca ggcagggcta
     2881 tctggagata tttttatggc aggtcatagt aaatgggcca acaccagaca tcgtaaagct
     2941 gcgcaggatg ctaagcgcgg taaaatcttc actaaaatca ttcgtgagct ggtaaccgcg
     3001 gctaagctgg gcggtggcga tccggacgct aacccgcgtc tgcgtgcggc ggtggataaa
     3061 gcactgtcta acaacatgac ccgtgacaca ctgaaccgcg caattgcacg tggtgtgggc
     3121 ggtgatgatg atgcaaacat ggaaaccatc atctacgaag gttacggtcc tggcggcacg
     3181 gcaatcatga ttgaatgtct gtctgacaac cgcaaccgta ccgttgctga agtgcgtcac
     3241 gcattcagca aatgtggcgg taacctcggc actgacggtt ccgtagctta tctgttcagc
     3301 aaaaaaggcg tgatctcctt cgagaaaggc gacgaagaca ccatcatgga agcagctctg
     3361 gaagcaggtg ctgaagacgt tgtgacatat gatgacggcg cgattgatgt ctacactgca
     3421 tgggaagaaa tgggtaaagt gcgcgacgct ctggaagcgg caggtctgaa agcagacagc
     3481 gcggaagttt ccatgatccc gtccaccaaa gctgatatgg atgcagaaac cgcgccgaaa
     3541 ctgatgcgtc tgatcgatat gctggaagat tgcgacgacg tgcaggaagt ttaccataac
     3601 ggtgaaatct ctgatgaggt tgcagcgact ctctgatgat gcctgctaaa cagcaaaacg
     3661 gagacgcgtg atggctatta ttctcggcat tgacccgggt tcgcgcgtga ccggctacgg
     3721 cgtcatccgc caggtaggta ggcaactgtc ctacctgggt agcggatgca tacgcaccaa
     3781 agtggatgat ttaccgtctc gtctgaagct catctatgcg ggcgtgacgg aaatcatcac
     3841 ccagttccag cctgattatt tcgccattga acaagtcttt atggcgaaga acgctgactc
     3901 agccctgaaa ctgggccagg cgcgcggcgt ggcgattgtt gcggcggtga atcaggaatt
     3961 gccggtattt gaatacgcgg cacgtcaggt aaagcaaacg gtggtaggta tgggtagtgc
     4021 cgaaaaaagc caggtgcagc atatggtccg caccttgctg aaactgcccg ctaatccaca
     4081 ggcggatgcc gccgatgcgc tggcgattgc catcacccac tgccacgtta gtcagaatgc
     4141 gatgcagatg agcgaatcgc ggctgaacct gacgagaggg cgactgcgtt aagttatacc
     4201 gcctcggtca gttccggctg aggcgtttca actccctccg cgtgaatcaa caccaaaccc
     4261 ggatgatgcc acaaactggc tggcgtgacg gttttacgct cccacggaat agaacctaag
     4321 aaccagaatt tccagaaagt gagttttgca ttcggattgc tatttaacag ttgtccaaac
     4381 gtctctattt cacccaccgg aatacatagc gcctctttat aaatatcaaa gacaaatttt
     4441 gaacagaact ggcgcgaaga ttcgtattta aaacccgtgt ggtaaagttt gcgtagccgg
     4501 gagggaacct gttcaacaat tcgttgtttt tgttgttctg tcagcccagc gtctaatcgc
     4561 tttatagcat agcgttgatt agacgaacgt ttaataaaac gagatagcgt ggtgatggtt
     4621 gaaaggggaa cccggctttc tgcaaccagg aagtcttcac cgttatgacc gataatgatc
     4681 ccgacgtgat tactccagca atttgatgca gctgatattt gaccaaataa ggcagcacct
     4741 atacatgtaa agacgatatc tccaatttca tattcagcag ggtaattaat attcacgttg
     4801 tcattccatt gaaatagata caaatatacg taatagcata aaaaaattat ttttcaggag
     4861 tcgcaatgta tatttttatt acgcatttct tcactgaata tgtaatattc aaatatttgc
     4921 ttccaatata acctgttgaa taaattatac tgtgccattt ttcagatcat cgggacacct
     4981 cgcaagtttt cttcatcctt cgctggatat ctatccagca tttttttatc atacagcatt
     5041 atctttgatt cattacgcag gagcgtcatg tgataggcag actcagaggc atcatcattg
     5101 aaaaacaacc cccgctggtg ttaattgaag tgggcggcgt aggctatgaa gtgcatatgc
     5161 cgatgacctg tttttatgaa ctccctgaag cgggtcagga agcgatcgtt ttcacccact
     5221 ttgtggtgcg tgaagacgcg caactgctgt acggttttaa caataaacaa gaacgcacgt
     5281 tgttcaaaga gttgatcaaa accaacggcg tcggcccgaa gttggccctg gcgatcctct
     5341 ccggaatgtc agcgcagcag ttcgttaatg ccgttgagcg tgaagaagtg ggggcactgg
     5401 tgaaactgcc gggtattggc aaaaaaaccg ccgaacgctt gattgttgaa atgaaagacc
     5461 gatttaaagg tttgcatggc gatctcttta cgccagccgc cgacctggta ctcacgtctc
     5521 ctgctagccc ggcgaccgac gatgctgaac aagaagcagt tgccgcgctg gtggcgctag
     5581 gctataaacc acaagaagca agccgcatgg tgagcaaaat cgctcgccct gacaccagca
     5641 gtgaaacatt aattcgcgaa gccctacgcg ccgcgttatg aggtaaagga tgattgaagc
     5701 agaccgtctg atttctgccg gtaccacttt gccggaagat gtagcagatc gcgccattcg
     5761 ccctaaatta ctggaagagt atgttggtca gccgcaggtt cgttcacaga tggagatttt
     5821 catcaaagca gcgaaactgc gcggcgatgc cctcgatcat ttgttgattt ttggtcctcc
     5881 ggggttgggt aaaactacgc ttgccaacat tgtcgccaat gaaatgggcg ttaatttacg
     5941 cacgacttct ggtccggtgc tggaaaaggc gggcgatttg gctgcgatgc tcactaacct
     6001 tgaaccgcat gacgtgctgt ttattgatga gatccaccgt ttatcgccag ttgttgaaga
     6061 agtgctgtac ccggcaatgg aagactacca actggatatc atgattggtg aaggtccggc
     6121 ggcacgctcc attaaaattg atttgccgcc gtttaccctg attggcgcaa ccacgcgcgc
     6181 aggttcgctg acatcaccgt tgcgcgaccg ttttggtatt gtgcaacgtc tggagtttta
     6241 tcaggtgccg gatctgcaat atatcgtcag tcgcagtgca cgctttatgg ggcttgagat
     6301 gagtgatgac ggtgcgcagg aagtagctcg tcgcgctcgc ggtacgccac gtattgccaa
     6361 ccgtctgctg cgtcgagtgc gtgatttcgc cgaagtgaag cacgatggca ccatctcggc
     6421 agatatcgct gctcaggcgc tggatatgtt gaatgtcgat gctgaaggtt tcgattatat
     6481 ggaccgcaaa ttgttgctgg cggtaatcga taagttcttt ggtgggccgg taggtctgga
     6541 taacctggcg gcagccattg gcgaagaacg tgaaaccatt gaagatgtac tggaacctta
     6601 tttgattcag caaggctttt tgcagcgtac accgcgcggg cgtatggcga cgacgcgggc
     6661 gtggaatcac tttggcataa cgccgccaga aatgccgtaa gtcggattgg tcgctcgcgc
     6721 cgcatccgac actggcagtg tgtctgatgc gacactgttc gcgtcttatc aggccagcca
     6781 gagatagcga accgtaggtc ggataaggcg ctcgcgccgc atccgacaaa tgtgttcagc
     6841 gatagattag ctggcctgct ttttcatcat actgataata aataacagtg ccgcacatag
     6901 caccaccgac ggacccgccg gcgtatcgta aaacgcggaa aaggttaaac cgccagttac
     6961 tgccaccatc cccaccaaaa cagcgacacc agccatctgt tccggcgtac gggcaaagcg
     7021 acgcgcagta gctgcaggaa taatcagcaa cgaagtaata atcaacgcac cgacaaattt
     7081 catcgctaca ccaatcgtca atgccgtcac cagcatcaac aacaatttca cttgctgtaa
     7141 tttcacacca tcaacaaacg ccagatccgg gctaatcgtc atcgacagca aattgcgcca
     7201 ttgccagaac aaaatagcca ccacgatgac cacgccaatc gcaatagaga tgagatcttc
     7261 tggcgtcact gccagtaaat caccgaacag gtaagccatc aaatcaacac gaatgttaga
     7321 catcagacta acgaccacta ggcccagcga cagggcactg tgcgccataa tccctaataa
     7381 cgtgtcgatc gccagctgtg gacgcttctc cagccatacc agaccgcccg ccagcagcag
     7441 cgtaacggca atcaccgcat agaatggatt cacgtccagc aacaaaccaa acgcgacacc
     7501 aagtaatgag gcatgagcca gcgtatcacc gaaataagac atacgacgcc agactacaaa
     7561 cgaacccagc ggacccgcgg cacaggcgag catgatcccg gctaaccaac cgggaaataa
     7621 taattcaatc atgagcgatc atttccccga cgcaaaacaa ttcgtccctg taaatcgtga
     7681 cgatgattat gatgatggcg atagataccc agttgttcag caccacgagg accaaacatt
     7741 gaaataaact ccggatgcag ggaaacaact tccggtgtgc cggaacaaca aatgtggtga
     7801 ttcaggcaca gcacttcatc ggtttttgcc atcaccagat gcagatcgtg agaaaccatt
     7861 aaaacgccac aatccagttc acgacgcaac tggtcaataa ggtcatataa cgccacttgg
     7921 ccatttacat ccacgccttg agtgggttca tccagcacta ataattgcgg gcaatttaac
     7981 aatgctcgcg ctaatagtac acgctgcgtt tcgccaccag agagcttttg catcggtgcg
     8041 ttaatcaggt gcccggcctg gacacgtttc agtgcaggca aaatatcttc tttatgtgta
     8101 ccagggcgta agcgtaaaaa acggtttacg gtcagtggca acgtggtgtc gagatacagc
     8161 ttctgcggta catagccgat gcgcagtttt ccgttgcgct tgataacccc ttcatcgggt
     8221 gttaccagcc cgagcactac ccgtaccagt gtcgacttac ctgcgccatt tggcccaagt
     8281 aaagtcaaaa tttttccagg tttaagttcc agcgacacat cagagaggac gcggcgttgg
     8341 ccaaaagaaa ccgagacatt ttccagggaa accagacttg tcatgttaat tttagtcttg
     8401 cagtagtcat gaaatgttat aatatcacac ttctcatatt cattacgatg attggtcgca
     8461 ttatgttaca taaaaaaacg cttcttttcg cagcattatc cgccgctctc tggggaggtg
     8521 caacacaggc cgcagatgcc gccgttgtcg cttcgcttaa acccgttggg ttcatcgctt
     8581 ctgccattgc tgatggtgta acagaaacgg aggttttact gcctgacggc gcttcagaac
     8641 atgattattc actgcgtcca tcggatgtaa aacgcttaca gaacgcggac ttagtcgttt
     8701 gggttggccc ggagatggaa gcgtttatgc aaaaaccggt aagcaaatta ccagaagcga
     8761 agcaggtaac gattgcgcag cttgaagatg tgaaaccgct actgatgaaa agtattcacg
     8821 gcgatgatga tgatcacgac cacgcggaaa aaagtgacga agatcaccat cacggcgatt
     8881 tcaacatgca tctttggctt tccccagaga tagcgcgggc tacagcggtt gcaatccatg
     8941 gaaaattagt ggaacttatg ccgcaaagtc gagccaaact tgacgccaac ctgaaggatt
     9001 ttgaggcaca attggcctca accgaaacgc aggttggtaa cgagctcgcg ccgctcaagg
     9061 ggaaaggtta tttcgttttt cacgatgctt acggctattt cgaaaaacag ttcggactga
     9121 caccgcttgg tcattttacc gttaaccctg agattcaacc tggcgcgcag cgtttacatg
     9181 aaataagaac acagttggtt gagcaaaaag caacctgcgt ttttgctgag ccacagttca
     9241 ggccagcggt cgttgagagc gtcgcacgag ggacatccgt tcgtatggga acgttggatc
     9301 ccctggggac gaatatcaaa ctgggtaaaa caagctattc agaattcctg agtcaattag
     9361 ccaaccagta tgcgagctgc ctgaaaggag attaatgagg aagtgattac gtgcaacaga
     9421 tagcccgctc tgtcgccctg gcgtttaata atttaccgcg accacaccgc gttatgttgg
     9481 ggtcgctcac cgttcttact ctggccgtcg ctgtctggcg gccctatgtt tatcaccgtg
     9541 atgccacgcc aattgtcaaa accattgagc tggaacagaa cgaaattcgt tcgctcttac
     9601 ctgaagccag tgagccgatt gatcaagctg cacaagaaga tgaagccatt ccccaggacg
     9661 aactggatga caaaatcgcc ggtgaagcgg gcgtgcatga atatgttgtt tccactggcg
     9721 atacgctaag cagcattctc aatcagtatg gtattgatat gggtgatatc acccaacttg
     9781 ctgctgccga caaagaattg cgtaacctga aaatcggtca acaactctcc tggacattaa
     9841 ccgcggacgg cgaactgcag cgcctcacct gggaagtgtc tcgtcgtgaa acccgaacct
     9901 atgaccgtac tgccgctaac ggttttaaaa tgaccagcga aatgcagcaa ggagagtggg
     9961 ttaacaatct gctgaaaggt accgtcggag ggagctttgt tgccagcgcc agaaacgccg
    10021 gtttaaccag cgccgaagtg agcgcagtga ttaaagccat gcagtggcaa atggatttcc
    10081 gcaaactgaa aaaaggcgat gaatttgcgg tattaatgtc tcgagaaatg cttgatggta
    10141 aacgtgagca aagccagctg ctgggcgtac gtttgcgttc agaaggtaaa gattattacg
    10201 caatccgcgc tgaggatggc aaattctacg atcgtaacgg tactggtctg gcgaaaggat
    10261 tcttgcgatt cccgacggcg aaacagttcc gtatctcgtc taactttaac ccgcgtcgaa
    10321 ctaatccggt gacaggtcgc gttgcaccac acagaggtgt tgatttcgcc atgccacaag
    10381 gtacgccagt gctttcagtg ggtgacggtg aagtggtggt tgccaaacgc agtggcgcag
    10441 caggttatta tgtggctatt cgtcatggtc gcagctacac cacgcgttat atgcacttgc
    10501 gcaagattct ggtgaaaccg ggacagaagg tgaaacgtgg cgaccgtatc gcgctttccg
    10561 gtaataccgg acgttcaact gggccgcatc tgcactatga agtatggata aaccagcagg
    10621 ccgtaaaccc gctgacggca aaactgccgc gtaccgaagg gctgaccggc tccgatcgtc
    10681 gcgaattcct ggcgcaggcc aaagagattg tgccgcagct acggtttgat taattaacat
    10741 ccattcgcag ccggtacgca gtcagtaccg gcttttttta tttggtgcgg ggcaagttgt
    10801 gccgctacac tatcaccaga ttgatttttg ccttatccga aactggaaaa gcatggaaac
    10861 gaaaaaaaat aatagcgaat acattcctga gtttgataaa tcctttcgcc acccgcgcta
    10921 ctggggagca tggctgggtg tagcagcgat ggcgggtatc gctttaacgc cgccaaagtt
    10981 ccgtgatccc attctggcac ggctgggacg ttttgccgga cgactgggaa aaagctcacg
    11041 ccgtcgtgcg ttaatcaatc tgtcgctctg ctttccagaa cgtagtgaag ctgaacgcga
    11101 agcgattgtt gatgagatgt ttgccaccgc gccgcaagcg atggcaatga tggctgagtt
    11161 ggcaatacgc gggccggaga aaattcagcc gcgcgttggc tggcaagggc tggagatcat
    11221 cgaagagatg cggcgtaata acgagaaagt tatctttctg gtgccgcacg gttgggccgt
    11281 cgatattcct gccatgctga tggcctcgca agggcagaaa atggcagcga tgttccataa
    11341 tcagggcaac ccggtttttg attatgtctg gaacacggtg cgtcgtcgct ttggcggtcg
    11401 tctgcatgcg agaaatgacg gtattaaacc attcatccag tcggtacgtc aggggtactg
    11461 gggatattat ttacccgatc aggatcatgg cccagagcac agcgaatttg tggatttctt
    11521 tgccacctat aaagcgacgt tgcccgcgat tggtcgtttg atgaaagtat gccgtgcgcg
    11581 cgttgtaccg ctgtttccga tttatgatgg caagacgcat cgtctgacga ttcaggtgcg
    11641 cccaccgatg gatgatctgt tagaggcgga tgatcatacg attgagcggc ggatgaatga
    11701 agaagtcgag atttttgttg gtccgcgacc agaacaatac acctggatac taaaattgct
    11761 gaaaactcgc aaaccgggcg aaatccagcc gtataagcgc aaagatcttt atcccatcaa
    11821 ataaaaaagc ctctcgcgag gagaggcctt cgcctgatga taagttcaag tttgcttcag
    11881 aatattcgaa atctgttgaa ctatcattga actataggcc ggatgtggcg ttttcgccgc
    11941 atccggcaac gtacttactc taccgttaaa atacgcgtgg tattagtaga acccacggta
    12001 ctcatcacgt cgccttgggt gacaatcacc aggtcaccag acatcaagta acctttatcg
    12061 cgcagcagat taaccgcttc gctggcagct gctacgccgt cattagcgct atcaaagggc
    12121 accggcgtaa cgccacgata gagagcagtc aggttcagcg tacgttcatg gcgcgacatg
    12181 gcgaaaattg gtagaccaga gctgatacgg gaggtcatca gcgcggtacg acccgattcg
    12241 gtcatggtaa tgatcgccgt aacgcccttc aggtggttag ctgcgtacat tgccgacatg
    12301 gcaatagctt cttccacatt gtcgaactga acgtccagac ggtgtttaga aacgttgatg
    12361 ctcgggattt tttccgcacc caggcaaacg cgcgccatgg ctgcaacagt ttctgacgga
    12421 tactgcccag cggcagtttc tgcagacagc atcacagcgt cagtaccatc cagaacggcg
    12481 tttgctacgt ccatgacttc tgcacgcgtc ggcatcgggt tagtaatcat tgactccatc
    12541 atctgggtcg ccgtgattac cgctcggttt agctgacgcg cacgacggat caacgctttc
    12601 tgaatgccga ccagttccgg gtcgccaatt tccacaccga ggtcgccacg tgcaaccatt
    12661 accacgtcag aggcgaggat gatgtcatcc attgcatcct ggctgcaaac ggcttccgca
    12721 cgttcaacct tggcaacaat tttcgcatca catcctgcat cgcgtgccag gcgacgggca
    12781 tagttcagat cttcgccaca gcgtgggaag gagacagcca ggtaatctac gccaatcaac
    12841 gccgcagtct taatgtctgc tttgtctttt tcggtcagcg cttcagccga caaaccgccg
    12901 ccaagtttgt tgataccttt attgttggag aggggaccac cgacggtgac ttcggtgaac
    12961 actttcatgc cctgaacttc cagtactttt aactggacgc gaccatcgtc cagcagcagg
    13021 atgtcaccag gcacgacgtc agtaggcagg cctttgtagt cgataccgac tttttctttg
    13081 tcgccttcac ctttacccag gttggcgtcg agcaggaatt tatccccaat attgaggaaa
    13141 actttgcctt ctttaaaggt ggatacacgg attttgggcc cctggaggtc acccagaata
    13201 gccacatgac gccccagttt tgcggcaatc tcacgaactt tatccgcgcg cattttgtga
    13261 tcttcaggcg agccgtgaga aaagttcata cgtacaacgt tggcacccgc cgcgataact
    13321 ttttcaagat tattatcgcg atctgttgct gggcctaacg tggtaacgat ttttgttctg
    13381 cgaagccttt tggacatgta atactccgtt gactgaaaca accaggtgtt gcttgaacat
    13441 gaaatccgaa tgaaataacg ccgcgatggc gataaattaa tctcattcgg atgtcgtata
    13501 agattaggac agtgacagtc gcttttagcg atcgtcactt aaattaagta actgcttatc
    13561 aaaacgcgat tccttcagcg cttccttgac ccgcttcaag ttatctctga attttgcacc
    13621 gcgtcgcaaa gtaaatcctg tcgccagcac atctatcacg gtcagctgtg caagtcgaga
    13681 aaccatgggc atataaatgt cagtatcttc cggtacgtcg agggtaattg ccagcgttgc
    13741 ttcccgggcg agcggggtac ccgcagaggt gagggcaatc accatggcgt cgttttcgcg
    13801 tgccagctgc gccagctcga ccagattttt tgttcttcct gtgtgagaaa tcagcaccac
    13861 cacgtctccg tcgctacaat tcatacaact catgcgttgc agcacgatat catcggagta
    13921 caccaccgga acattaaaac gaaagaactt attcatcgca tcgtgggcaa cggcggcaga
    13981 agagcctaat ccgaaaaagg cgattttttt tgcctgagtg agcaagtcga cggcgcggtt
    14041 gatggcagat ttatccagtg aatgacggac atgatcgagc gttgccattg cggactcaaa
    14101 tattttcccc gtgtatgatt caacgctgtc atcttcattg acgttgcgat taacataggg
    14161 agtgccattc gccagacttt gtgccagatg aagtttaaaa tcaggaaaac cgcgcgtgtc
    14221 catgctgcga cagaaacgat tcaccgtcgg ttcgctaaca ttggcttcca gcgccagagc
    14281 agcaatactc gaatggatcg cgttatcggg cgaagccaga atgacctcgg caactttgcg
    14341 ctctgatttg ctcaaatgtt ccagctgaga ctggattttt tccagcatat tcatgatgta
    14401 aagagactca cgggtaatga cgatttccgc actgaaagaa atcgaaatgc agttttgtca
    14461 gatattacgc ctgtgtgccg tgttaatgac aaaagcagat aaaaaagttg ttattttttt
    14521 cataacatga tcagtgtcag atttttaccg aatggaaaac gatgattttt ttatcagttt
    14581 tgccgcgctt tgcgcgcttt tcccgtaatc gcacgggtgg ataagcgttt acagttttcg
    14641 caagctcgta aaagcagtac agtgcaccgt aagaaaatta caagtatacc ctggcttaag
    14701 taccgggtta gttaacttaa ggagaatgac atggcggtaa cgcaaacagc ccaggcctgt
    14761 gacctggtca ttttcggcgc gaaaggcgac cttgcgcgtc gtaaattgct gccttccctg
    14821 tatcaactgg aaaaagtcgg tcagctcaac ccggacaccc ggattatcgg cgtagggcgt
    14881 gctgactggg ataaagcggc atataccaaa gttgtccgcg aggcgctcga aactttcatg
    14941 aaagaaacca ttgatgaagg tttatgggac accctgagtg cacgtctgga tttttgtaat
    15001 ctcgatgtca atgacactgc tgcattcaac cgtctcggcg cgatgctgga tcaaaaaaat
    15061 cgtatcacta ttaactactt tgccatgccg cccagcactt ttggcgcaat ttgcaaaggg
    15121 cttggcgagg caaaactgaa tgctaaaccg gcacgcgtag tcatggagaa accgctgggg
    15181 acgtcgctgg cgacctcgca ggaaatcaat gatcaggttg gcgaatactt cgaggagtgc
    15241 caggtttacc gtatcgacca ctatcttggt aaagaaacgg tgctgaacct gttggcgctg
    15301 cgttttgcta actccctgtt tgtgaataac tgggacaatc gcaccattga tcatgttgag
    15361 attaccgtgg cagaagaagt ggggatcgaa gggcgctggg gctattttga taaagccggt
    15421 cagatgcgtg acatgattca gaaccacctg ctacaaattc tctgcatgat tgcgatgtct
    15481 ccgccatctg acctgagcgc agacagcatc cgcgatgaaa aagtgaaagt actgaagtcc
    15541 ctgcgccgca tcgaccgctc caacgtacgc gaaaaaaccg tacgcgggca atatactgcg
    15601 ggcttcgccc agggcaaaaa agtgccggga tatctggaag aagagggcgc gaacaaaagc
    15661 agcaatacag aaaccttcgt ggcgatccgc gtcgacattg ataactggcg ctgggccggt
    15721 gtgccattct acctgcgtac tggtaaacgt ctgccgacca aatgttctga agtcgtggtc
    15781 tatttcaaaa cacctgaact gaatctgttt aaagagtcgt ggcaggatct gccgcagaat
    15841 aaactgacta tccgtctgca acctgatgaa ggcgtggata tccaggtact gaataaagtt
    15901 cctggccttg accacaaaca taacctgcaa atcaccaagc tggatctgag ctattcagaa
    15961 acctttaatc agacgcatct ggcggatgcc tatgaacgtt tgctgctgga aaccatgcgt
    16021 ggtattcagg cactgtttgt acgtcgcgac gaagtggaag aagcctggaa atgggtagac
    16081 tccattactg aggcgtgggc gatggacaat gatgcgccga aaccgtatca ggccggaacc
    16141 tggggacccg ttgcctcggt ggcgatgatt acccgtgatg gtcgttcctg gaatgagttt
    16201 gagtaatatc agcgcttatc ctttatgggt attttaccgg taacatgatc ttgcacagat
    16261 tgtagaacaa tttttacact ttcaggcctc gtgcggattc acccacgagg ctttttttat
    16321 tacactgact gaaacgtttt tgccctatga gctccggtta caggcgtttc actcataaat
    16381 cctctgaatg aaacgcgttg tgaatcatcc tgctctgaca actcaatttc aggagccttt
    16441 atgaatccac aattgttacg cgtaacaaat cgaatcattg aacgttcgcg cgagactcgc
    16501 tctgcttatc tcgcccggat agaacaagcg aaaacgtcga ccgttcatcg ttcgcagttg
    16561 gcatgcggta acctggcaca cggtttcgct gcctgccagc cagaaaacaa agcctctttg
    16621 aaaagcatgt tgcgtaacaa tatcgccatc atcacctcct ataacgacat gctctccgcg
    16681 caccagcctt atgaacacta tccagaaatc attcgtaaag ccctgcatga agcgaatgcg
    16741 gttggtcagg ttgcgggcgg tgttccggcg atgtgtgatg gtgtcaccca ggggcaggat
    16801 ggaatggaat tgtcgctgct aagccgcgaa gtgatagcga tgtctgcggc ggtggggctg
    16861 tcccataaca tgtttgatgg tgctctgttc ctcggtgtgt gcgacaagat tgtcccgggt
    16921 ctgacgatgg cagccctgtc gtttggtcat ttgcctgcgg tgtttgtgcc gtctggaccg
    16981 atggcaagcg gtttgccaaa taaagaaaaa gtgcgtattc gccagcttta tgccgaaggt
    17041 aaagtggacc gcatggcctt actggagtca gaagccgcgt cttaccatgc gccgggaaca
    17101 tgtactttct acggtactgc caacaccaac cagatggtgg tggaatttat ggggatgcag
    17161 ttgccaggct cttcttttgt tcatccggat tctccgctgc gcgatgcttt gaccgctgcc
    17221 gctgcgcgtc aggttacacg catgaccggt aatggtaatg aatggatgcc gatcggtaag
    17281 atgatcgatg agaaagtggt ggtgaacggt atcgttgcac tgctggcgac cggtggttcc
    17341 actaaccaca ccatgcacct ggtggcgatg gcgcgcgcgg ccggtattca gattaactgg
    17401 gatgacttct ctgacctttc tgatgttgta ccgctgatgg cacgtctcta cccgaacggt
    17461 ccggccgata ttaaccactt ccaggcggca ggtggcgtac cggttctggt gcgtgaactg
    17521 ctcaaagcag gcctgctgca tgaagatgtc aatacggtgg caggctttgg tctgtctcgt
    17581 tatacccttg aaccatggct gaataatggt gaactggact ggcgggaagg ggcggaaaaa
    17641 tcactcgaca gcaatgtgat cgcttccttc gaacaacctt tctctcatca tggtgggaca
    17701 aaagtgttaa gcggtaacct gggccgtgcg gttatgaaaa cctctgccgt gccggttgag
    17761 aaccaggtga ttgaagcgcc agcggttgtt tttgaaagcc agcatgacgt tatgccggcc
    17821 tttgaagcgg gtttgctgga ccgcgattgt gtcgttgttg tccgtcatca ggggccaaaa
    17881 gcgaacggaa tgccagaatt acataaactc atgccgccac ttggtgtatt attggaccgg
    17941 tgtttcaaaa ttgcgttagt taccgatgga cgactctccg gcgcttcagg taaagtgccg
    18001 tcagctatcc acgtaacacc agaagcctac gatggcgggc tgctggcaaa agtgcgcgac
    18061 ggggacatca ttcgtgtgaa tggacagaca ggcgaactga cactgctggt agacgaagcg
    18121 gaactggctg ctcgcgaacc gcacattcct gacctgagcg cgtcacgcgt gggaacagga
    18181 cgtgaattat tcagcgcctt gcgtgaaaaa ctgtccggtg ccgaacaggg cgcaacctgt
    18241 atcacttttt aagacgacaa atttgtaatc aggcgagaga aaactctgat gaaaaactgg
    18301 aaaacaagtg cagaatcaat cctgaccacc ggcccggttg taccggttat cgtggtaaaa
    18361 aaactggaac acgcggtgcc gatggcaaaa gcgttggttg ctggtggggt gcgcgttctg
    18421 gaagtgactc tgcgtaccga gtgtgcagtt gacgctatcc gtgctatcgc caaagaagtg
    18481 cctgaagcga ttgtgggtgc cggtacggtg ctgaatccac agcagctggc agaagtcact
    18541 gaagcgggtg cacagttcgc aattagcccg ggtctgaccg agccgctgct gaaagctgct
    18601 accgaaggga ctattcctct gattccgggg atcagcactg tttccgaact gatgctgggt
    18661 atggactacg gtttgaaaga gttcaaattc ttcccggctg aagctaacgg cggcgtgaaa
    18721 gccctgcagg cgatcgcggg tccgttctcc caggtccgtt tctgcccgac gggtggtatt
    18781 tctccggcta actaccgtga ctacctggca ctgaaaagcg tgctgtgcat cggtggttcc
    18841 tggctggttc cggcagatgc gctggaagcg ggcgattacg accgcattac taagctggcg
    18901 cgtgaagctg tagaaggcgc taagctgtaa aagtcaaaat gcccgatcta ggatcgggca
    18961 tttttgtagc gtttttttgc cgggtttaac cctgtacttt tacctgtccg gcggcgtgct
    19021 tcgcgcgttc aatggcgtca acaacactct ctgaagtagc cagtaccacc cccagatgac
    19081 ggctgccatc aatttccggc ttaccaaata aacgaatctg caaatctgcg cctacggcat
    19141 tctgcacatt atcaaacgtg acattctgac tggtcagttg tggcagaata acggcagaag
    19201 ctgcaggacc atactgacgg atcccgccaa caggaagtcc gaggaaggca cgtacatgca
    19261 gggcaaactc tgagagatct tgagaaatta acgtcaccat cccggtatca tgtggacgag
    19321 gggagacctc actgaaaatc acctcatcac cacagacaaa cagctcgaca ccaaacagac
    19381 cataaccgcc cagtgccagc accactttac gggcaatctc ctgcgcacgt tcaagggcaa
    19441 gcgggctcat ttgctgtggt tgccaggatt cacggtagtc gccatcttcc tggcgatgac
    19501 ctactggtgc acagaaatgg acgccatcca ccgcgctgac ggttagcagg gtaatttcga
    19561 agtcaaactt aacgacgcct tcaacaatta cgcggcccgc tccggcgcga ccgccttgct
    19621 gagcgtattc ccatgcctga gcaagttgct ctgcagagcg aataaacgtc tgccccttgc
    19681 cggaagagct catcaccggt tttacaatgc agggatagcc aatggcggca accgcctcgc
    19741 ggaaaaggct tttgctatcg gcaaaacgat aagtggaagt gggcagctgc agctcttctg
    19801 ccgccaggcg acggataccc tcgcgattca tcgttaattt cgttgcgcga gcgcagggga
    19861 caacattcag tccttcctct tcaagttgga tcagcatatc ggtggcaata gcttcgatct
    19921 ccggcacgat ataatgtggt ttttccagtt caaccacacg gcgtaatgca tcaccatcaa
    19981 gcatattaat gacatgggag cgatgcgcga catgcatggc tggtgcgtcg gcatagcgat
    20041 cgacggcaat cacctctacg ccgagacgct gacactcgat tgccacttct ttacccagtt
    20101 caccggagcc taataacatc acgcgagttg ctgccggacg cagcgcagtg cctaataacg
    20161 tcataacgaa tatcccctga ttaattccgc gcgcagtata aacgaaaacg tttgcgtgtg
    20221 tctttattgc tgatgttgat ttctcaaccg aaaagaaata tactgtataa aatctcagtt
    20281 attatgagag gctgcaaatg gcggttgaag ttaaatacgt agtcattcgt gagggcgaag
    20341 agaaaatgtc gtttaccagc aaaaaggaag ccgatgctta tgacaaaatg cttgatacgg
    20401 cggatctgct tgacacctag ctgacaaatt ctccagtgca aatggaagac gagcaacgtg
    20461 aagccctttc gctatggctg gcagaacaaa aagaggtgct gagcaccatt ctgaaaaccg
    20521 gcaaattacc gtccccacag gtcgtcggtg ctgaatccga agaggaagat gcctcacacg
    20581 cggcctgaag caacttgcgc tcctgacaat ttgtaccatg ctttctcctg aatcattgtg
    20641 ctatggagaa aaacatgaaa aaaagagggg cgtttttagg gcggttgttg gtttctgcct
    20701 gcgcatcagt tttcgctgcc aataatgaaa ccagcaagtc ggtcactttc ccaaagtgtg
    20761 aaggtctgga tgctgccgga attgccgcga gcgtaaaacg tgattatcaa caaaatcgcg
    20821 tggcgcgctg ggcagatgat caaaaaattg tcggtcaggc cgatcccgtg gcctgggtca
    20881 gtttgcagga cattcagggt aaagatgata aatggtcagt accgctaacc gtgcgtggta
    20941 aaagtgccga tattcattac caggtctgcg tggactgcaa agcgggaatg gcggaatatc
    21001 agcggcgtta atggcttctg ataattcgat tgaccgttgg cggatgcggc gcgagtgcct
    21061 tgtccgtccc agaacgcacc acaatcgtag gcctgagaag acgcaacagc gtcgcatcgg
    21121 gcaatcagca tcaaacaatg ccactcaaaa cgacaaacta ctttcaccta aaataccctt
    21181 atcacctgat atacagtaaa taccttacct ggcgcgaatt tctttgaata acggcctacc
    21241 gccccgctta tttgcgcatc ttttgtctcg atatacattt tctgacatcc tcatccgcta
    21301 tgctacaggc actaaaaatg gttattgagg agcaaatatg gctaactggt taaatcaact
    21361 gcaatccctg cttgggcaaa gcagttcttc tacctcctcg tctgcggatc agggattggg
    21421 caaactgtta gtgtcaggcg cattaggcgg actggctggg ctgctggtcg caaataaatc
    21481 agcacgtaaa cttcttacga aatatggcac caacgcgtta ctggttggcg gcggagcggt
    21541 agcgggtacg gtgctgtgga ataaatacaa agataaaatt cgcgcggcgc atcaggacga
    21601 accgcagttt ggcgcgcaaa gtacgccgct ggatgagcgt acggaacgtt tgatccttgc
    21661 gctggtcttt gccgctaaaa gtgatggtca tattgatgcc aatgaacgtg cggcaatcga
    21721 ccagcaattg cgtgaagccg gcgtggaaga gcaggggcgt gtactcattg agcaggcaat
    21781 cgaacaaccg ctggatccac aacgcctggc taccggggtc cgcaatgaag aagaggcgct
    21841 ggaaatctat ttcctgagct gcgcggctat tgatattgac cattttatgg aacgcagtta
    21901 cctgaatgca ctgggtgacg cgctgaaaat cccccaggat gttcgtgaag gcattgaacg
    21961 cgatctcgaa cagcaaaaac gcactttggc ggaataattc cgctctctgg atagcctgaa
    22021 gttgactatt tcgggctatc cagaaccgca cagccatttg aacaggctga ttttctctgt
    22081 ctgcataaca ttcgatatcc gcgccattac ctgtcatcat ctaagcaatg acgaccctgt
    22141 ttcgcttgca tccccggtga gttttgccac ccttataaga tgtttccaac cagaaagaac
    22201 aataacatgc taccaaaagc cgcccgcatt ccccacgcca tgacgcttca tggcgatacg
    22261 cgcatcgata attactactg gctgcgggac gatacgcgtt ctcagccaaa agtcctggac
    22321 tacctgcaac aagaaaatag ttacggtcat cgggtgatgg cctcacaaca agccttgcag
    22381 gatcgcatct taaaggaaat catcgaccgc attccgcaaa gagaagtttc tgcgccctac
    22441 ataaaaaatg gctaccgcta tcggcatatt tatgaaccag gctgtgaata tgctatctac
    22501 cagcgtcaat cggcattcag cgaagagtgg gatgagtggg aaacattgct cgatgccaac
    22561 aagcgcgcag ctcatagtga gttttattcg atgggcggaa tggcgattac gcccgataac
    22621 accattatgg cgctggcaga agattttctt tcccgacgcc agtacggcat tcgttttcgt
    22681 aatctggaaa caggtaactg gtacccggaa ctgctggata acgttgaacc cagctttgtc
    22741 tgggcaaatg actcctggac tttctactat gttcgcaagc atccggtgac gctgctgcct
    22801 tatcaggtct ggcgtcacgc catcggtacg ccagcatcgc aagatacact gatctacgaa
    22861 gaaaaagacg atacctatta tgtcagcctg cataaaacga cgtcgaagca ctatgtcgtc
    22921 attcatctgg ccagcgccac caccagtgaa gttcgcctgc tggacgcgga aatggccgat
    22981 gccgagccgt ttgtttttct gccgcgccgc aaagatcacg aatacagcct tgatcactac
    23041 cagcatcgtt tttatctgcg ttccaatcgc cacggcaaaa actttggctt ataccgtacc
    23101 cgtatgcgtg atgagcaaca gtgggaagag ttaattccgc cacgcgaaaa catcatgctg
    23161 gaagggttta cgctgtttac cgactggctg gtggttgaag agcgtcagcg cgggttaacc
    23221 agtttgcgcc aaattaaccg caagacccgg gaagtcattg gcattgcctt tgatgatccg
    23281 gcctatgtga cctggattgc ctacaatcca gaacctgaaa ccgcgcgatt gcgttatggt
    23341 tattcttcca tgaccacacc agacactttg tttgaactgg atatggatac cggtgagcgt
    23401 cgtgtattaa aacaaacaga agtgcctggt tttgatgcgg cgaattaccg cagtgaacac
    23461 ctgtggatag tcgcccgtga tggcgtcgaa gttccggttt cgctggtcta tcatcgcaaa
    23521 cattttcgca aaggacacaa cccgttgctg gtgtatggct atggttctta cggcgcaagt
    23581 attgatgccg atttcagttt tagccgcttg agtttgttag atcgtggctt tgtctacgcc
    23641 attgtccatg ttcgcggcgg tggtgagctg gggcaacaat ggtacgaaga cggtaaattt
    23701 ctgaagaaga aaaatacgtt taatgattat cttgatgcct gcgatgcatt gttaaaactg
    23761 ggctatggct ctccttcgct ctgttatgcg atgggcggaa gtgcgggggg catgttgatg
    23821 ggcgttgcaa ttaatcaacg cccggaatta ttccacggcg ttatcgccca ggtaccgttt
    23881 gttgatgttg taacaacgat gcttgatgaa tcaattcctc ttaccactgg tgagtttgaa
    23941 gagtggggaa atccgcagga tccgcaatat tacgagtaca tgaaaagcta cagcccgtat
    24001 gacaacgtca ccgcacaggc ttatccgcat ttactggtaa cgaccggttt acacgattct
    24061 caggtgcaat attgggaacc ggcaaaatgg gtcgctaaat tgcgcgagct gaaaaccgat
    24121 gaccatcttt tattgctctg taccgacatg gactcaggcc atggcggtaa atctggccgc
    24181 tttaaatcgt acgaaggcgt agcgatggaa tatgcttttc tggtcgcgct ggcgcaggga
    24241 acattacccg ctacgcctgc ggattaagta ttttccagat aatgtttcag tgttaaacgc
    24301 agctccgggc tcatgctgtc caggttatta aataaccagc gcagatagcc cggatagcgt
    24361 tcggcaacgt cggaaaccgc tttgccacgg tatttgccaa aggtgaaggt tgtcatcaac
    24421 gacggacgtc cggtgatatc ggccatctgt tctgccgtcc agccggaggt gttcataata
    24481 tcgataagca acgcggcggt gatataacag tcatacaacg cgcggtgatg atgcaggccc
    24541 agcggtgtct gtacattgag cttgcgtgtt ttatataacg ccatattgct gtacttgagc
    24601 cctggccaca aacgacgggc cagtttcata gtgcaaatcc actcaccggg catctcaggc
    24661 agtacgcggc ggtcaaagct ggcgttgtgc gcgacatacc attcactacc gtaatagtgt
    24721 gggatcacat cttcaatcca cggtttatcg gcgaccatgg cttcggtgat gcgatgaatc
    24781 gccatcgctt gtggactaat aggacgatcg gggcgcacca ggaggctcat ggggttgacg
    24841 atttttccgt caatgacatc aacagaggca atctcaacga tccctccctg caaaccgcag
    24901 gtttctgtat cgataatgcg caacatggca tgatccaggc cgaaaaagcc tagcgtaatg
    24961 gaatgatatc gccttgccaa ccctgtgatg aacgctgacc gactaacaat aatgaaccac
    25021 gatcggcgcg aacgatgaga cgaccatatt catcccatag tgcactgtta ccacgggcat
    25081 ttgccatcag cacggcaatc gagtactgat gtgaaaaaaa ttgtaagcga cgggccgacg
    25141 ccagcaggtc gggttctccc agacattgcc ctgtcgtaaa cagtgaacag gtcgggtcca
    25201 tatccatacc ttgcggttgt tcatcgacca ccgtgatggt tctggaacga cggcccagac
    25261 atgcaccatg actctgatgg tagttcccag gcgtttttcg ccacggtgcg aacaccgcaa
    25321 ttccacgaat aaagcgatcg ttatattcaa cgggaaggcc ggcaatgatg gtcattcgct
    25381 aggtcgttgc ggcataacaa agcgggtcta acagtgaaag atcgggtggg gcaggaaggg
    25441 cacgtcgcga ataatcacac cccagtaaag aaagcgatgg aaaaactaac agctgacatt
    25501 gttgcctggc ggcggccctc acaaactcca gatgatgagc tacctgttcg gtgagtgagg
    25561 ttttacgagg ctcatattgc gctgctgcaa ctttccagaa cgacataatg acttcctttt
    25621 caaaatgact gcaaattatg ggggcggttt agaaaatact cataatttag caggcgttat
    25681 gtaagaaagt gtaactctgt ataagtttta tttaagtttg ggctcgtaag gtaaacgtga
    25741 cagattgacc gaagccaaac ggtgggcaat aagccgctcg cgaaaccagc tgcgcaaatg
    25801 ttctggctgt tcacgttcaa ccgcttcagc gatcaccggc atattgtagc gttctttaaa
    25861 agccaccccg gccgccgcca aatcgacatt cactttatcc atttctgttt gatccagttt
    25921 agccagattc ttcagcattc ttaatctcct catcattcgc gggcaaaagt tactaagagt
    25981 gagcctggat ggcaagccgg aattgatgat cctcgactca gacattagac aaggttatgc
    26041 tctgtaaaaa agttattaca aaaaggaata gataatatgg cttcaactgc acgctccctt
    26101 cgctacgcgc tggcaatcct gaccacttcg ttggtgacac cttccgtctg ggcacatgcg
    26161 catttaacgc atcagtatcc tgcggc
//
LOCUS       DAZPCS010000040        25659 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZPCS010000040 DAZPCS010000000
VERSION     DAZPCS010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25659
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.040"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..1358)
                     /gene="yeaG"
                     /locus_tag="ACHUQ7_001680"
     CDS             complement(<1..1358)
                     /gene="yeaG"
                     /locus_tag="ACHUQ7_001680"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFR1135130.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQDPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNKSEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQ"
     gene            1794..2540
                     /gene="mipA"
                     /locus_tag="ACHUQ7_001681"
     CDS             1794..2540
                     /gene="mipA"
                     /locus_tag="ACHUQ7_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFR1135131.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            2630..3391
                     /locus_tag="ACHUQ7_001682"
     CDS             2630..3391
                     /locus_tag="ACHUQ7_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFR1135132.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHV
                     QQHAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(3442..4326)
                     /locus_tag="ACHUQ7_001683"
     CDS             complement(3442..4326)
                     /locus_tag="ACHUQ7_001683"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFR1135133.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(4410..5405)
                     /gene="gapA"
                     /locus_tag="ACHUQ7_001684"
     CDS             complement(4410..5405)
                     /gene="gapA"
                     /locus_tag="ACHUQ7_001684"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFR1135134.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            5747..6160
                     /gene="msrB"
                     /locus_tag="ACHUQ7_001685"
     CDS             5747..6160
                     /gene="msrB"
                     /locus_tag="ACHUQ7_001685"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFR1135135.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            6202..6474
                     /locus_tag="ACHUQ7_001686"
     CDS             6202..6474
                     /locus_tag="ACHUQ7_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFR1135136.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            6844..7920
                     /locus_tag="ACHUQ7_001687"
     CDS             6844..7920
                     /locus_tag="ACHUQ7_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFR1135137.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVTEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            7947..9326
                     /locus_tag="ACHUQ7_001688"
     CDS             7947..9326
                     /locus_tag="ACHUQ7_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1135138.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMDVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLITM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSNIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            9343..10388
                     /locus_tag="ACHUQ7_001689"
                     /pseudo
     CDS             9343..10388
                     /locus_tag="ACHUQ7_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            10409..11245
                     /locus_tag="ACHUQ7_001690"
     CDS             10409..11245
                     /locus_tag="ACHUQ7_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFR1135139.1"
                     /translation="MLADIRYWENDATNKYYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFSTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSIEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            11250..12197
                     /locus_tag="ACHUQ7_001691"
     CDS             11250..12197
                     /locus_tag="ACHUQ7_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFR1135140.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMSTGGDA
                     INEATIISRLGHRTALMSRIGKDASGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFPQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNFAEEKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            12207..13187
                     /gene="ydjG"
                     /locus_tag="ACHUQ7_001692"
     CDS             12207..13187
                     /gene="ydjG"
                     /locus_tag="ACHUQ7_001692"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HFR1135141.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTALGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            13324..14082
                     /locus_tag="ACHUQ7_001693"
     CDS             13324..14082
                     /locus_tag="ACHUQ7_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFR1135142.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAVLTENIHFYKRASSFYEEKLLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            14200..15558
                     /locus_tag="ACHUQ7_001694"
     CDS             14200..15558
                     /locus_tag="ACHUQ7_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1135143.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLYAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLEGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFRLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(15651..16292)
                     /gene="pncA"
                     /locus_tag="ACHUQ7_001695"
     CDS             complement(15651..16292)
                     /gene="pncA"
                     /locus_tag="ACHUQ7_001695"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFR1135144.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLGGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(16303..17319)
                     /gene="ansA"
                     /locus_tag="ACHUQ7_001696"
     CDS             complement(16303..17319)
                     /gene="ansA"
                     /locus_tag="ACHUQ7_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFR1135145.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAPNYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNALAHAGVIDGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(17486..19342)
                     /gene="sppA"
                     /locus_tag="ACHUQ7_001697"
     CDS             complement(17486..19342)
                     /gene="sppA"
                     /locus_tag="ACHUQ7_001697"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFR1135146.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            19503..20054
                     /locus_tag="ACHUQ7_001698"
     CDS             19503..20054
                     /locus_tag="ACHUQ7_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFR1135147.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            20171..21214
                     /gene="selD"
                     /locus_tag="ACHUQ7_001699"
     CDS             20171..21214
                     /gene="selD"
                     /locus_tag="ACHUQ7_001699"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFR1135148.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            21219..23180
                     /gene="topB"
                     /locus_tag="ACHUQ7_001700"
     CDS             21219..23180
                     /gene="topB"
                     /locus_tag="ACHUQ7_001700"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFR1135149.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            23307..24347
                     /locus_tag="ACHUQ7_001701"
     CDS             23307..24347
                     /locus_tag="ACHUQ7_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR1135150.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWGIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(24464..25657)
                     /gene="gdhA"
                     /locus_tag="ACHUQ7_001702"
     CDS             complement(24464..25657)
                     /gene="gdhA"
                     /locus_tag="ACHUQ7_001702"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005094017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFR1135151.1"
                     /translation="MERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGG
                     MRFHPSVNLSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGKVMRFCQTLMT
                     ELYRHLGADTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEA
                     TGYGLVYFTEAMLKRHGMGFEGMSVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTV
                     VDESGFTKEKLARLIEIKASRDGRAADYAKEFGLVYLEGQQPWSVPVDIALPCATQNE
                     LDVDAAHQLIANGVKAVAEGAYMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEM
                     AQNAARLGWKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAML
                     AQGVI"
BASE COUNT         6517 a         5971 c         6624 g         6547 t
ORIGIN      
        1 tgctgttcca ggacgtagaa cagatggacc gggtttgccg ccacttctac atgatcgaag
       61 ttgaacacgc gggagaggat cttaaacgca aaacgcgtcg acagaccgtt catcccttca
      121 tcgacaccgg cgtagtcacg atattcctga tacgacttgg ctttcggatc ggtgtctttc
      181 agactttcgc catcataaac ccgcatcttt gaataaatgc tggagttttc tggctctttc
      241 aggcgcgaaa gaatggaaaa acgtgacagt gtttcgagcg tgccaggggc gcatggggcg
      301 tgagtcaatt cactgtgatt aagcaatttc tcgtagattt tgatctcttc ggaaatgcgc
      361 aagcaatacg gcaccttcac gatgtaaaca cggtcgagga aggcttcgtt gtttttgtta
      421 ttacggaaag tgacccattc ggacttgttc gagtgggcga gaataatccc gttgaacggc
      481 agggcggaga taccttccgt cccgttgtag ttaccttcct gagtggcggt taacaaggga
      541 tgcagcactt ttatcggtgc tttaaacatc tcaacgaatt ccatgatccc ctgattggcg
      601 cggcacagcg caccggaata gccgtaggcg tccgggtcat tctgcgcgta gtgttcgagt
      661 ttacgaatat cgactttccc aaccagcgcg gagatgtcct ggttgttctc atcaccgggt
      721 tccgttttgg cgatagcaat ttgttgcaga attgacggcc agaccttcac tacccggaac
      781 ttagtgatat cgccaccaaa ttcatgcagg cgttttgccg cccatggcga catgatggtg
      841 ccgagataac ggcgaggaat gccatactct ttttccagaa tctgagcatc ttcctgcgga
      901 ttgaaaagac agaacggatg atcgttgacc gggctacgct caccgttggc gctcaataca
      961 taaatcggta cgagctgcat taatgatttc agtcgctcag caagcgatga tttaccccca
     1021 cccacaggcc ccagcagata caggatttgt ttcttctctt ccagcccctg agccgcgtgt
     1081 ttcagataag agacaatctg ttcaatcgcg tcttccatgc cgtaaaactc ttcaaatgcc
     1141 ggataacgtg caatgacccg gttagaaaag agtcgagaaa gtctggggtc ctgggctgta
     1201 tcgaccatga caggctcacc gatagccatc aatagccgct cagccgcgtt ggcataagca
     1261 ctgcgatctt gccgacaagt ggtaagaaac tcctgcagtg tgaactcttc gtccttggca
     1321 gcttcatatc gctggcgata gtgatcgaat atattcatgg tatgccgtcc tttcgttttt
     1381 tagcacaggt taagagccgt tcatatgaat agtagaggct cccggaagag gtaaactgaa
     1441 cgacgtctct tgctaaaaca cagcaactct catgccattt tatcgacgat gagaacaacg
     1501 tgcccggtga tacaccttca aaattaagcg tagatggcaa atggaaaact tgcctgcacg
     1561 gctttcttaa tttcaatggc atatcaacaa ctcatccata aattttgcat aattaatgta
     1621 aagaccaggc tcgccagtaa cgctaaatcc atttggctgt aagcgcggtg tcatccgcgg
     1681 caggaaaatt aaacagttac tttaaaaaat gaaaacgtaa aaaggttggg tttcgatgta
     1741 ttgacgggta aactttgtcg cccgctaaac atttgtttaa ggaatgatta attgtgacca
     1801 aactcaaact tctggcactt ggagtgctta tcgcaacgtc tgcaggcgta gcgcacgctg
     1861 aaggtaaatt ttccctgggc gcaggcgtag gtgtcgttga gcacccatat aaagattacg
     1921 ataccgatgt ttacccagta ccggtaatca actatgaagg cgataacttc tggttccgtg
     1981 gcttaggtgg tggttactac ctgtggaatg acgcaacgga taaactttca attaccgctt
     2041 actggtcgcc gctttacttc aaagcgaaag acagtggcga tcaccaaatg cgtcacctgg
     2101 atgaccgtaa gagcaccatg atggctggtc tgtcttatgc tcactttacc cagtacggtt
     2161 acctgcgtac caccctggct ggcgataccc tggataacag caacggcatt gtctgggata
     2221 tggcctggtt gtatcgttac accaacggtg gcctgaccgt gactccgggt attggtgtgc
     2281 agtggaacag cgaaaaccag aacgaatact attatggcgt atcgcgcaaa gagtccgctc
     2341 gcagcggtct gcgtggctat aacccgaatg acagctggag cccttacctt gagctgagcg
     2401 ccagctacaa cttcctcggc gactggagtg tttacggtac cgcgcgctac acccgtctgt
     2461 ctgatgaagt tactgacagc ccgatggtgg ataaatcctg gactggcctg atttctaccg
     2521 ggatcaccta caaattctga taatgcatct ttccagggca ttttgtgcat aaaaacaggg
     2581 cgtcacgccc tgttttgcat tacggtggtg caatacctgg gaggaaacaa tgcaacaaaa
     2641 aatgattcaa tttagtggcg atgtctcact gccagccata gggcagggaa catggtatat
     2701 gggcgaagat gccagtcagc gcaaaacaga agttgctgca ctacgcgcag gcattgaact
     2761 cggtttaacc ctcattgata ccgccgaaat gtatgccgat ggcggtgccg aaaaggtggt
     2821 tggggaagca ttaaccggtc tgcgagagaa ggtctttctc gtctctaaag tctatccgtg
     2881 gaatgctggc gggcaaaaag cgataaatgc atgcgaagcc agtttacgcc gtctcaatac
     2941 tgactatctc gatctctact tattacactg gtctggcagt ttcgcttttg aagagactgt
     3001 tgctgcgatg gaaaaattga tcgcccaggg aaaaatccgt cgctggggcg tttctaacct
     3061 tggttatgcc gatatgcagg aactctggca gctgccgggg ggaaatcagt gtgccactaa
     3121 tcaggtgctt taccatctcg gttcacgagg gattgagtac gatctactcc cctggtgcca
     3181 gcaacagcag atgccggtga tggcttacag tccattagcc caggccgggc ggttgcgcaa
     3241 tggactgtta aaaaacgcgg tagtcaacga aattgcacat gtccaacaac atgcagctgc
     3301 gcttgaggtc gaactttcgt cagcggaatt agctatgctg gataaggcat atccggcacc
     3361 aaaaggaaaa actgcgctgg atatggtgtg atgttgatac gtgccggatg catcgcaccc
     3421 ggcacacaac attaacgcaa attaacgttt ggcaacgcga atggattgcg ccagatgtgc
     3481 aggtttctct ttggtgactt tttgcgtttc tgaagcgtaa gccgtttcta cacaaacaaa
     3541 ggtcttgtag ccatcgtccg gcatgtcgcc catgctaact gaaagcgccg gtcccgggtt
     3601 ccagccgaca acgttcagat gatgctggtg gcctacggcg ataatacgat tcagcgcttc
     3661 atcattaatc acgctgcaat cttgcggatt cagatacacg cgatcggtac ggtcagggaa
     3721 ggtctgaata ccgtcggtca gtacatcttc tttcgcgtca ttcactttat caatgaagcg
     3781 atcgcccagc ccactgacgc ttaccttagc gatatcaccc acgttaaagt aggtatgcag
     3841 ggcagaggtg gtttcaaatt cgccatgtga ttcaagatcg atttcacagg ttttacccac
     3901 gcggaaatgc gctaacagcg taaagtcgtg cggccagaat tttttcgtct cttcgctttg
     3961 tgtcaattca aaagtcagcg ctacgccatc agcatcttca tgatgtgatt tcagcgtcca
     4021 cggcaggttg cgggcaaaac cgtgcgcagg cagaccttgt tgtgccgccg gaccaaacca
     4081 cggccagcaa accggtacgc caccgcgaat agcgacgcca tttttgaacg gtgtgttgtt
     4141 gctcaaccac agaacttctt cttcacccgc aggtttccac gagagaaggt gtgcgccctg
     4201 taatgcaaaa gaggctttta cctggggatg atcgaccaca atgaggtcca gttcatccag
     4261 tttacgacgg gagaggacag gggagatttg ttcgatgacc ggaagggcaa aaattttctt
     4321 aatcatgacg cagtccttta acttcatttt atcaggtaaa aaaaagagcg accgaagtcg
     4381 ctctttttag atcacagtgt catctcaact tatttggaga tgtgagcgat caggtccaga
     4441 actttgttgg agtaaccggt ttcgttgtcg taccaggata ccagtttcac gaagttgtcg
     4501 ttcagagcga taccagcttt agcatcgaac acggaagtgc aaacttcgcc gttgaaatcg
     4561 gtagatacta cgtcatcttc ggtgtagccc agaacgcctt tcatttcgcc ttcagcagca
     4621 gctttaacgg cagctttgat ctgctcgtaa gttgcagctt tttccagacg aacggtcagg
     4681 tcaactacgg atacgttcgg ggtcggaacg cggaacgcca taccagtcag tttgccattc
     4741 agttctggca gtactttacc tacagcttta gcagcaccgg tagaggacgg gatgatgttc
     4801 tgggaagcgc cgcggccgcc gcgccagtct ttgtgagacg ggccatcaac ggttttctga
     4861 gtagcggtag tagcgtgaac ggtggtcatc agaccttcga tgatgccgaa gttatcgttg
     4921 ataactttag ccagcggagc caggcagttg gtggtgcagg aagcgttgga aacgatgtcc
     4981 tggccagcat atttgtcgaa gttagcgcct ttaacgaaca tcggagtgtt gtctttagac
     5041 ggaccagtca taaccacttt cttcgcacca gcggtgatgt gtttacgagc agtttcgtca
     5101 gtcaggaaca gaccagttgc ttcagcgaca acgtcaacac ccacttcgtc ccatttcagg
     5161 ttagccggat cacgttcagc ggtaacacgg atttttttac cgttaacgat cagatgaccg
     5221 tctttcactt caacggtacc gtcgaaacgg ccgtgagtgg agtcatattt cagcatgtat
     5281 gccatgtaat cagcgtctaa caggtcgttg attgcaacga tctcgatgtc agaacgtttc
     5341 tgagcagcac ggaaaacaat gcgaccgata cggccaaaac cgttgatacc tactttgata
     5401 gtcatatatt ccaccagcta tttgttagtg aataaaaggt tgcctgtaaa attacaaaaa
     5461 ccttacgcag cgtcaagcgg aatcgtgtca atcattgcga caaatcaatc ctgtgcctaa
     5521 gcattacgcg actgactcgc ctcacttttc ctttgggctt gagaccacat ggggtcggcg
     5581 ccccgaattt taaagggcaa ttacgataaa aatgtgattt tcatcacgat ttaggtgcag
     5641 ccatttcgtc gagatcaagt ttaggacaaa attttgccag ttggtgttaa tgttttgtta
     5701 gaatcagtca cgtaatgtga gcacgattaa agtgagatgt gagtaaatgg cgaataaacc
     5761 ttcggcagaa gaactgaaaa aaaatttgtc cgagatgcag ttttacgtga cgcagaatca
     5821 tgggacagaa ccgccattta cgggtcgttt actgcataac aagcgtgacg gcgtatatca
     5881 ctgtttgatc tgcgatgccc cgctgtttca ttccgaaacc aagtatgatt ccggctgtgg
     5941 ctggcccagt ttctacgaac cggtgagtga agaatccatt cgttatatca aagacttgtc
     6001 acatggaatg cagcgcatag aaattcgttg cggtaactgt gatgcccatc tggggcatgt
     6061 ctttcccgac gggccgcaac caacaggcga acgttattgt gttaactctg cttctttacg
     6121 cttcactgat ggcgaaaacg gcgaagaaat caacggttga aaaaacgatt cagcaactta
     6181 ttccacagga gcggaaaaat tatgaatctt gatgacatta tcaacagcat gacgcctgag
     6241 gtataccagc gtttgtcgac cgccgttgaa ctggggaaat ggcctgatgg cgttgcgttg
     6301 accgaggaac aaaaagagaa ttgcctgcaa ctggtgatgc tgtggcaagc ccgccataat
     6361 accgaagcac agcatatgac aattgacaca aatggtcaga tggtcatgaa gagcaaacag
     6421 cagttaaaag aagattttgg tatctcagca aagcctattg ccatgtttaa gtaagtggtt
     6481 catccccttt ttgccgcggc tatgaagggg atagccaaca atgaccccac gactcgtctc
     6541 aattatctcg acccgacctg gctaagtccc agtccgatct cttgcagtcg cgttcacgcc
     6601 tgaatgactt ctcctgaact taagtctcag acctatttgg ccggtaatcc ctctcgaatt
     6661 tgctcttttt gcgtcccgcc ttccaccaac ctgtaacaga cccagccatt ttctgcgtaa
     6721 ttgtgatttg agttgtaaaa tgcaggtcat tatctgtgtt gtgcagattt aatattgacc
     6781 tcaaaaatca tgtgggtaag tatgatggcc atctaagcaa aacaaaacac agaggatgac
     6841 gcaatgaaag cactggctcg gtttggcaag gcctttggcg gctacaagat gattcatgtc
     6901 ccacaaccca tttgtggccc ggaagacgta gtgactgaaa ttaaagccgc ggcaatctgc
     6961 ggcgcagaca tgaagcacta caatgtcgat agcggttctg atgagtttaa ctctatccgc
     7021 ggccatgagt tcgcaggttg tattgcgcag gttggtgaaa aagtcaaaga ctggaaagtg
     7081 gggcaacgcg tcgtgtcgga taacagcggt cacgtttgcg gcgtttgtcc ggcctgtgaa
     7141 cagggtgatt ttctgtgttg tacagaaaaa gtaaaccttg gtctggataa caatacctgg
     7201 ggcggtggtt tttccaaata ttgtctggtt cctggtgaaa ttctcaaaat tcatcgtcat
     7261 gcgttgtggg aaatccctga tggtgttgat tatgaggacg cagccgtact tgaccctatc
     7321 tgtaatgcct ataaatccat cgcgcagcaa tcgaaattcc ttcctggtca ggatgttgtc
     7381 gtcattggca ctggcccact cgggctgttt tccgtacaaa tggcgcggat tatgggggcg
     7441 gtaaatatcg tcgtcgttgg tctgcaagaa gatgtggcgg tccgcttccc ggttgcaaaa
     7501 gaactggggg cgacggcagt agtaaatggt tctaccgaag atgtggtggc gcgctgccag
     7561 caaatttgtg gcaaagataa tctgggactg gtgattgaat gctccggtgc caatatcgca
     7621 ctgaaacaag ccatcgaaat gctccgcccg aatggggaag tggtacgcgt tggaatgggc
     7681 ttcaaacctc ttgatttctc gattaatgac attaccgcct ggaataaaag catcattggg
     7741 catatggcct atgactccac ctcatggcgt aacgctatcc ggctattagc cagcggcgct
     7801 atcaaagtca aaccgatgat cacgcatcgt atcggcctgt cgcaatggcg cgaagggttt
     7861 gatgcgatgg tcgataaaac cgcaatcaaa gtgatcatga cttacgactt tgatgaataa
     7921 ctactctcct gaaggaacgt tatcgcatgg aacagataac aaaaccgcat tgtggtgcac
     7981 ggctggatcg cttaccggat tgccgctggc attcgtcaat gtttgccatc gtcgcgtttg
     8041 gcttgcttgt ctgctggagt aatgccgttg gtggcttgat cctcgcgcag ctgaaagcgt
     8101 tgggctggac agataattcc actacagcca cattctcagc aatcacgacc gccggaatgt
     8161 ttctcggtgc gctggtgggc ggcatcattg gcgacaaaac cggtcgcaga aatgcgttca
     8221 tcctctatga ggccattcat attgcctcga tggttgtcgg tgctttctca ccgaatatgg
     8281 attttcttat tgcctgccgt tttgtgatgg acgttgggct gggagcttta ctggttacac
     8341 tgtttgctgg tttcaccgaa tatatgcccg gtagaaatcg cggaacgtgg tccagtcggg
     8401 tttcctttat tggcaactgg tcatatccgc tctgctcact gattgcgatg ggactcacgc
     8461 cgctgattag tgcagagtgg aactggcggg tacaactgct tattcctgca atattgtcgc
     8521 ttatcgctac ggcgctggcc tggcgctact ttcctgaatc cccgcgctgg ctggaatcgc
     8581 gcggacggta tcaggaagcc gagaaagtca tgcggagtat agaagaaggc gtcatacgcc
     8641 agacgggtaa acctttgccg cctgtggtta ttgctgatga cggtaaagcg ccacaagcgg
     8701 tgccgtattc agccttactg acaggagtat tactgaaacg cgtgatatta ggctcttgtg
     8761 tgctgattac catgaacgtt gtgcagtaca cactaattaa ctggttgcca acaatattca
     8821 tgacccaggg gattaattta aaagactcga ttgttttaaa taccatgagt atgtttggcg
     8881 cgccatttgg tatttttatt gccatgctgg tgatggataa aattccgcgt aaaacaatgg
     8941 gtgtggggct attaatcctg attgcggtgc tcggatatat ctattcactg caaaccagta
     9001 tgttgctcat aacgctgatt ggtttcttcc tgattacttt cgtctatatg tacgtttgct
     9061 atgcctcggc agtgtatgtc cctgaaatct ggccgacaga ggccaaactc cgtggctccg
     9121 gtctggcgaa tgcggtaggg cgaatcagtg gtattgccgc accttatgcc gttgcagtgc
     9181 tgctcagtag ttatggtgta acgggagtct ttattcttct gggggctgtt tcaaatattg
     9241 tcgcaattgc tatcgccacc attggaattg aaaccaaagg tgtctccgtt gaaagtttaa
     9301 gtattgatgc agtagccaat aaataatacg ggtggattca aaatgaaaaa ttcaaaagca
     9361 atattgcagg tgccgggcac aatgaaaatt atttcagcag aaataccagt gcctaaagaa
     9421 gatgaagttt tgattaaagt agaatatgtc ggtatttgtg gttcagatgt acatggtttt
     9481 taatcaggcc cgtttattcc gcctaaagac ccaaatcaag aaattggcct gggtcatgaa
     9541 tgcgccggga cggttgtggc tgtgggaagc cgcgtgcgca aatttaaacc gggggatcgg
     9601 gtaaatatcg aacctggcgt tccttgcggt cactgtcgtt actgtctgga aggcaaatat
     9661 aacatctgcc cggacgttga ttttatggcg acacaaccca actaccgcgg cgcattaacg
     9721 cactatctgt gtcatccgga gagctttact tacaaactgc ccgacaatat ggacacgatg
     9781 gaaggggcgc tggtggagcc tgccgcagtc gggatgcatg ccgcgatgct ggcagatgtt
     9841 aaaccgggta agaagataat tattctggga gcaggttgta ttggtttgat gacgttgcaa
     9901 gcgtgcaaat gcctgggcgc aacggaaatt gccgtcgttg atgtactgga aaaacgtctg
     9961 gcaatagggg aacagcctgg tgcgacagtg gttattaatg gcgcaaaaga ggacactatt
    10021 gcacgctgtc agcaatttac cgaagacatg ggcgcagata ttgttttcga aacagcgggt
    10081 tctgcggtca ccgttaaaca ggcaccttta atctggtaat gcgcggcggt aaaattatga
    10141 ttgttggtac tgtacccggc gattcggcaa tcaatttcct caaaatcaat cgcgaagtca
    10201 ctatccagac ggtattccgc tatgccaatc gttatccggt cacgattgaa gctatttctt
    10261 cagggcgatt cgatgtgaaa tcgatggtga cgcatattta cgattatcgg gatgtacaac
    10321 aggcatttga agagtcagtt aacaacaaac gcgacattat taaaggcgtt attaaaatta
    10381 gcgattaatt aatacgggag aacagaacat gctcgcagat atcagatatt gggaaaacga
    10441 tgccacaaat aaatattatg caattgccca ttttaacgta tggaatgcag aaatgttgat
    10501 gggcgttaaa gacgcagccg aagaagcgaa atccccggtt attatttctt ttagtacagg
    10561 ttttgtcggt aacacctcat ttgaagattt ctctcacatg atggtatcaa tggcacaaaa
    10621 agcaacggtg ccggtaataa ctcattggga tcatggtcgg agtattgaga ttattcataa
    10681 cgcctggact catggtatga attcattaat gcgtgatgct tctgcatttg atttcgaaga
    10741 aaatattcgt ttaaccaaag aggctgttga tttctttcat ccgctgggta ttccggtaga
    10801 ggcggaatta gggcatgtcg gtaatgaaac cgtttatgag gaagcgctgg cgggctatca
    10861 ctataccgat cctgaccagg cggctgaatt tgttgaacgt acgggctgtg actcattggc
    10921 tgtcgccatc ggcaaccagc atggggttta tacgtcagag ccgcaattga actttgaggt
    10981 cgtcaaacgc gtacgcgatg ctgtttctgt tccgctggtt ttgcatggcg catcggggat
    11041 cagtgatgcc gacattaaaa ctgcaatttc gttgggtatt gcgaaaatca acattcatac
    11101 ggagctctgt caggccgcaa tggtagcagt gaaagaaaac caggatcagc ctttcctgca
    11161 tttagaacgt gaagtacgta aagctgtaaa agaacgggca ttggaaaaaa ttgaactgtt
    11221 cggttctgat ggcaaagcgg aataatgata tggataatct cgacgttatt tgtataggtg
    11281 ccgctattgt tgatattcca ttacaaccgg tcagtaaaaa tatctttgat gtagattctt
    11341 atcctcttga aagaatcgca atgagcaccg ggggcgatgc aataaatgaa gcaacaatta
    11401 tttctcgtct ggggcatcgt accgcattaa tgagccgcat tggtaaagat gcctcagggc
    11461 aatttattct cgaccattgt cgcaaagaga atattgatat tcaaagcctg aaacaggacg
    11521 tcagcataga tacatctatt aacgttggac tggtgacgga ggatggcgag cggacgtttg
    11581 tcacgaaccg taacggcagt ttgtggaaat taaatattga cgatgttgat tttgcgcggt
    11641 ttcctcaggc gaaattatta tcactggcca gtattttcaa cagtcctcta ttggatggta
    11701 aagcgctaac agaaattttt acgcaagcga aagcccggca gatgatcatt tgtgccgata
    11761 tgatcaaacc gcggttgaat gaaacgctgg atgatatttg cgaagcattg agttatgtcg
    11821 attatctgtt tccaaatttt gccgaggaaa aattactcac agggaaagag acactggatg
    11881 aaattgctga ctgctttctt gcgtgcggcg taaaaacggt ggtgattaaa acgggtaaag
    11941 acggctgctt tatcaagcgt ggtgacatga cgatgaaggt gccggcggtc gcaggaataa
    12001 ccgccatcga caccattggc gcgggcgata actttgcttc aggttttatt gcggcactgt
    12061 tagaaggcaa aaatctgcgt gaatgcgcac gctttgccaa tgcaacggcg gctatctcgg
    12121 ttctaagcgt cggtgccacc accggcgtaa aaaacagaaa gctggtcgaa caattgctgg
    12181 aagaatacga aggataatga aggcaaatga aaaagatacc tttaggcacg acggatataa
    12241 cgctttcgcg aatggggttg gggacatggg ccattggcgg cggtcctgca tggaatggcg
    12301 atctcgatcg gcaaatatgt attgatacga ttcttgaagc ccatcgttgt ggcattaatc
    12361 tgattgatac tgcgctagga tataactttg gcaatagtga agttatcgtc ggtcaggcgt
    12421 taaaaaaact gccccgtgaa caggttgtag tagaaaccaa atgcggcatt gtctgggaac
    12481 gaaaaggaag tttattcaac aaagttggcg atcggcagtt gtataaaaac ctttccccgg
    12541 aatctatccg cgaagaggta gaagccagct tgcaacgtct gggtattgat tacatcgata
    12601 tctatatgac gcactggcag tcggtgccgc cattttttac gccgatagct gaaactgtcg
    12661 cagtgcttaa tgagttaaag gctgaaggga aaattcgcgc tataggcgct gctaacgtcg
    12721 atgctgacca tatccgcgag tatctgcaat atggtgaact ggatattatt caggcgaaat
    12781 acagtattct cgaccgggca atggaaaacg aactgctgcc gctatgtcgt gataatggca
    12841 ttgtggttca ggtttattcc ccgctagagc agggattgtt gaccggcact atcactcgtg
    12901 attacgttcc gggcggcgct cgggcaaata aagtctggtt ccagcgtgaa aacatgctga
    12961 aagtgattga tatgcttgaa cagtggcagc cactttgtgc tcgttatcag tgcacaattc
    13021 ccactctggc actggcgtgg atattaaaac agagtgattt aatctccatt cttagtgggg
    13081 ctactgcacc ggaacaggta cgcgaaaatg tcgcggcact gaatatcaac ttatcggatg
    13141 cagacgcaac attgatgagg gaaatggcag aggccctgga gcgttaaata ctcaacacga
    13201 ttcacctctg tttctgtgaa aactgtgatg aaaatctgtg attcaaatag gttattttga
    13261 aagtaaacat cggttatgcg ataatcgcgc taatgtgatg taaaaagata caggggtgta
    13321 atcgtggcgg caaaagacag gattcaggca attaagcaaa tggttgccaa cgataaaaag
    13381 gtgacagtct caaatttgag tgggattttt caggtaaccg aagaaaccat tcgccgcgat
    13441 cttgagaagc tggaagatga aggctttttg accagaacct atggtggtgc tgttttaaat
    13501 acagcggtgt tgacggagaa tatccatttt tataagcgcg cttcatcgtt ttatgaagag
    13561 aagctgctta ttgcacgcaa ggcactaccc tttatcgaca ataaaaccac catggcagcc
    13621 gattccagta gtaccgtaat ggaattgctc aaattgttac aggaccgtag tgacctgacg
    13681 ttgctaacca actcggcgga agcgattcat gttctggctc agtcagaaat taaagtcgtt
    13741 tcaacgggtg gggaactaaa caaaaataca ctttccctgc aaggaagaat tactaaagag
    13801 atcatcagcc gctaccatgt cgatatcatg gtaatgagtt gtaaaggtct tgatattaac
    13861 agcggcgcgc tggactctaa tgaagcggaa gctgaaatca aaaagacaat gatccgtcag
    13921 gcgacagaag ttgcgttatt ggttgatcac tctaagtttg atcgtaaagc ttttgtccag
    13981 ttagctgact ttagtcatat taattatata ataactgata aatcaccggg tgcagagtgg
    14041 atagcatttt gcaaagacaa taatatccag ctggtgtggt aatttaagca cacgttgtca
    14101 atattatttg ttcctgcgac tggaaataaa atgatgataa agatgcggca agtgaatttg
    14161 ccgcaattgc taccttttat agcaaatccg aggcacccaa tggaacaata tgatcaaatt
    14221 ggcgcaagac tggaccgctt gcctttggcc cggtttcatt atcgtatatt tggtataata
    14281 agctttagtc tgttattaac ggggtttttg agttactcag gtaatgtcgt cttagcaaag
    14341 ctggtaagca atggatggtc aaataatttc ctctatgccg cctttacctc ggcattaatg
    14401 tttggttatt tcatcggctc acttactggt gggtttattg gtgactactt tgggcggcgc
    14461 agggcgtttc gcataaatct tctcatcgtc ggtattgctg caacaggggc cgcttttgtc
    14521 cctgatatgt actggctcat tttctttcgc ttcctgatgg gaacaggaat gggggcgctg
    14581 attatggttg gctatgcctc atttacggag tttatccccg cgacggtgcg tggaaaatgg
    14641 tccgcgcggc tctcatttgt tggtaactgg tcgcccatgc tgtctgcggc gataggcgtg
    14701 gtggttatcg ctttttttag ttggcgaata atgtttcttc tgggtggtat tggcatactg
    14761 ttagcctggt ttctctcagg taaatacttt atcgagtcgc cacgatggct ggaagggaaa
    14821 gggcaaatcg ccggtgcaga aagccaactt cgtgaagtag agcagcaaat tgaaagggag
    14881 aagagtattc gtttacccca gcttactttg aaccagagca acagcaaggt taaggtaatc
    14941 aagggtacct tcaggctcct gtttaaaggg gaaatgttac gacgtacatt agttgcgata
    15001 actgttttaa ttgcaatgaa catttcgctt tataccatta ccgtatggat accgaccata
    15061 tttgttaact ccggcattga tgtcgataaa tcaatattaa tgaccgctgt tattatgatt
    15121 ggtgctccgg taggaatatt tattgcggca ttaattattg atcattttcc tcgtcgatta
    15181 tttggctccg ccttacttat tattattgcc gtgttaggct atatctattc aagtcagact
    15241 acagagtggg cgattttaat ctatggtctg gtgatgatct tctttttata catgtatgtt
    15301 tgcttcgcgt cggcggttta tatcccggag ctttggccaa cgcatttacg cctgcgcggt
    15361 tcgggtttcg ttaatgccgt cggacggatc gtcgcagttt tcacgcccta tggcgttgcg
    15421 gcattattaa cacattatgg gtcgatcacg gtgtttatgg tgcttggtgt catgttagtg
    15481 ctctgtgcgc tggttctctc catttttggc atcgaaacgc ggaaggtgtc gctggaagag
    15541 atttctgagg tgaattaaat ctttcgttga tggcatcaat tgccagatgt accactgacg
    15601 tgttttattt ggcctacaat gccagtacag aattgtaggc cagcgtaaaa ttacccctgc
    15661 gtctcttccc agtctgccag cgtatatagc gttgccccag ctgctgacat ctccataaac
    15721 gcgtgtgcac tgtcctgggg ctggatattc acgccacgac agccatcggt aatcacgttt
    15781 accttataac ctaactgtaa cgcgtccagc acggtaaact tcacgcaata gtcagtagcc
    15841 aggcccataa cgatcaattc atcgatttca tgatcgcgca accagtcatc aagcgcggtt
    15901 ttctgccgac gtccgttatc aaaaaaggca ctgtaactgt caactaaagg attttcgcct
    15961 ttatggaaca ccgctgcgat ctctttttga ttcagtaacg gatgtaattg tgcgccttca
    16021 ctgttctgca cacagtgttc aggccagaaa gtttgccgca ggccgccgag ttggcctggc
    16081 gtataaggct ctacgccgtg ctggctggca aagctgccgt gattcgccgg atgccagtcc
    16141 tggctggcga taaccgcttc tccgcgcgac tggcaccagt caataaggcg gttagcaacc
    16201 tccaccgtac tgtcaccttc cggtacggcg agcgcgccac cagcacagaa atcattttgt
    16261 aaatcgacca gtaacagggc gcgatggggc attgcagtct ccttaatcat ccggcgtcag
    16321 ttcgccgcgc aggttttggc tcatggcctt gcgaatggtt tcagtatcca gttcctggct
    16381 cagcaggtaa tgcagtttgg ttagcgtggc ttctacagtc atatctgcac cgtcaataac
    16441 gccggcatgg gcgagggcgt taccggtggc ataaccactc atattcactt taccggacat
    16501 acattgtgtc aggttgacca ccacaatacc gcgatcgctg gcttcttgta attcctgcag
    16561 gaaggctttg ttttgtggcg cattacccac gccataggag cgcagaatca atgctttcac
    16621 cggttggcgc agaaaattgc gcacgacgtc agcagaaatc cctggataaa tcgtcaccac
    16681 gccaattggt tgtggcgtga ttggatgaac gattaattcc ccttcaccgt gcggggcggg
    16741 tggcgtattc aaacgacgaa tatggatacc agcttccagt aacggaggaa ggtttggaga
    16801 ggcaaacgca tcaaaaccat cggcatgggc tttggtagtg cggttgccgc gatacaatcg
    16861 gttattgaaa aagagcgtta cttcgttgat cggataattc ggcgcaacgt acaacgcatt
    16921 cagcagatta atttgtccgt cagagcgtaa ctcagccagc gggatttgtg accctgtcac
    16981 aatgaccggt ttaccgagat tctcgagcat gaacgacagc gcagaggcgg tatacgccat
    17041 cgtgtcggta ccgtgtagaa tgacaaaacc atcatagtcg tcatagtgcg ctttaatatc
    17101 ttcagcaata tgctgccagt cttccggcgt catatcagaa gaatccatca acggcgtata
    17161 ttcatgaatg gtgaaatctg gcatctccgg gcgatggaat tccggcatca gcgccagttg
    17221 gcgttgtaga tgacctgaca ccggaatata accctgctcg gaacgctgca tcccgatggt
    17281 cccgcccgtg taggcgacgt aaattgattt cttttgcatg atattgatat gaatgatatc
    17341 gaaagagcaa aagtatatac gtgaggcagg aggagcgcac ctgatactat gcggataagg
    17401 aaatatgggg cgagacagcg ggaagaggat tcgtcgaggt aaaaaataat gtggccgacg
    17461 atctgtcggc cactcagtac aagacttaac gcacgttggc gcaggtcagg caaaacgcat
    17521 aacggttttg tgggtcgtta aacgcggcca gcttgtcgct ttcactttta acggtagagg
    17581 ctaccgaggc aagcggtgca ggtaacatgg cctggaacgc atctggcaac attgcccgga
    17641 cagaaccaga catgttgtcc atcactttgt cgaagaaggt cggttcatca acgtagtatt
    17701 ccagatgcca ctgtttcact tttgcgagct ctgctgcttt ggcaaccgca tcatcgaaat
    17761 ccccgagact atcgaccagc ccgttagctt ttgcatcctg accggtccag acgtggccct
    17821 gggcgatttt atcaatttgc tccggcgtcg aatgacgcgc atcagcaacc agcgtgataa
    17881 agcgtttata gccattctca atgcttaatt gcatcatctg ctgcgcttcc ggcggcagtg
    17941 ccctggtgat agaaacatcc gccagcggtg aagttgagac accatcagta tgaacaccaa
    18001 tcgaatccag actattttct acggtggtga tcacgccgaa gataccgata gaaccggtca
    18061 gggtgctggg gttagccaca atgtaattag ctggcgtgga aatccagtaa ccaccagatg
    18121 ccgccatgcc gcccatcgat acaaccacag acttacccgc tgcccgggct gctgccagtt
    18181 cagcgcgaat cacttcagac gcggtaacgc tgccgcctgg gctattaaca cgcaggacaa
    18241 tcgctttcac tttcgggtca aggcgagcgt cgcggatttg tgccgcagtg gtatcaccgc
    18301 caacattccc ctgagtttcc tcgccatcca taattgcgcc attagcgaag acgacaccga
    18361 tgctgtcacc ggtatctgcc ggcgttttca atgcgtaatc gtaataactg atggcgcgat
    18421 aatttttatc agtcttactc cagccgaact ctttggtcag agctttttcg atttcggcac
    18481 tcgatgccag tgcatcgacc agcttgtttt ccagtgcata tttcgcggta tcgccaccgg
    18541 ttttggttaa accctcaagc aacccttgcg cgccagggaa tacctgctga gcagggatct
    18601 gccggttagc ggcaacagta ttcagatagt tttgccacag ctcaccaatc cagcggctgt
    18661 cagcttcgcg ggctgccggt gacatatcat cacgaataaa cggttcaacg gcagatttat
    18721 acgtacccac gcggaacaca tgggtggaaa ctttcagctt atccagcaac gatttgtagt
    18781 acagaccgtt ggtagcaaaa ccgtgcagat caaccacgcc ttgcggtgac agccaaattt
    18841 tattggcgaa actggcgaga taatattgcc cctggctgta gttctcgcca acggcataaa
    18901 ccggtttccc gctgtcacga aactctttca gggctttgcc gatgtactgc atagacggct
    18961 ggtcgccgcc tgcgaagttt ttcagatcca tcacgatacc ggtgatattg cggtcgtcct
    19021 tcgcctggcg aatggtgttg acgatatcaa acagtgagtt ttcctgcaga cgatcggaac
    19081 tggcaccaag cagctggcgg cttaatttac taaaccgctg agaactgtct ggtttatcga
    19141 cgatcacacc agaaatgtcc agcagcagtg cgccacgact ggccgtttct ttcgaatcac
    19201 caccactgac ctgcatccag atccccacac caaccagtac gaggaaaata aagaacaggt
    19261 taagtaccat ttcacggacg aaattcagca gacgccacgt ccatttaaaa aatccggcaa
    19321 taaatcgcca aagggttcgc atgtattctc ccaacttaag gtcacacctg tcacaggcgc
    19381 aatagatgtg gttatcgtaa tgacccaacc ggcacttgtc agcaggaatt gcggactgtg
    19441 ctgtaacaaa atccactctc gtgttaattt tgtgaataaa tatcacgaca ggagttaatc
    19501 aaatggatgc actcgaacta ttgatcaatc gccgtagcgc ctcccgcctg gctgaacccg
    19561 cgccaacggg tgaacaactg caaaacatcc tgcgtgcggg tatgcgtgcg ccggaccata
    19621 agtccatgca accgtggcat ttttttgtga ttgaagggga agggtgcgag cgtttcagcg
    19681 ccgtactgga acagggggcg attgctgccg gtagtgatga taaagctatc gacaaagccc
    19741 gtaatgcgcc gttccgcgca ccgctcatca tcacggtggt ggcgaaatgc aaagaaaatc
    19801 ataaagtccc gcgatgggaa caggaaatgt ctgccggatg cgcggtcatg gcgatgcaaa
    19861 tggcagccgt tgcccaggga tttggcggca tctggcgcag tggcgcatta actgaaagtc
    19921 cggtagtgcg tgaagcattc ggttgccgtg agcaggataa aattgtcggt tttctctacc
    19981 tcggcacgcc gcagttgaaa gcatctacgt cgattaacgt cccggacccg acgccgtttg
    20041 taacttattt ctgataattg tcgcgaaact gtctggattg tgagcaaagg cgcaggaatt
    20101 cagacactct cacttatcac ttcacggaat gagggttacc atacccggat tgcattgaca
    20161 ggagatgtcc atgagcgaga actcgattcg tttgacccaa tacagccacg gagctggttg
    20221 cggctgtaaa atttccccaa aagtgttgga aaccatcctg cacagtgagc aggcgaagtt
    20281 tgttgatccg aatttgcttg tgggtaatga aacccgcgac gatgcggcgg tgtacgatct
    20341 gggcaatggc accagcgtta tcagtaccac cgacttcttt atgccgatcg ttgataatcc
    20401 tttcgatttt ggccgcattg cggcgactaa cgccatcagc gatatcttcg cgatgggggg
    20461 caaaccgatt atggcgattg cgatcctcgg ctggccgatt aacaaacttt ccccggaaat
    20521 tgcccgcgaa gtaaccgaag gtggacgcta tgcatgccgt caggcgggta ttgcgctggc
    20581 tggcggtcac tccatcgatg cgccggagcc gatttttggt ctggcggtaa cggggatcgt
    20641 accgaccgag cgggtgaaga aaaacagtac cgcacaagcc ggatgcaaac tgttcctgac
    20701 gaaaccgctg gggatcggcg ttcttaccac ggctgagaaa aaatcactgt tgaaaccaga
    20761 acatcaggga ctggcgacgg aagtgatgtg ccggatgaac atcgcaggcg cgtcctttgc
    20821 caacatcgaa ggcgtaaaag cgatgaccga cgttacgggc tttggtctgc tgggccactt
    20881 gagcgaaatg tgtcaggggg ctggtgtgca ggcacgcgtc gactatgaag cgatcccgaa
    20941 actccccggt gttgaagagt acattaagtt gggcgcagta cctggcggca ctgaacgtaa
    21001 ctttgccagc tacggtcatc tgatgggtga aatgccgcgt gaagtgcgcg atctgctgtg
    21061 cgatccgcaa acttctggcg gtttgctgct ggcggtcatg ccggaagcag aaaatgaggt
    21121 taaaactaca gccgccgagt ttggcattga actgacggcg attggcgaac tggtgccagc
    21181 gcgcggcggt cgtgccatgg ttgagatccg ttaatttaat gcggttgttt attgccgaaa
    21241 aaccgagtct ggcgcgcgcc attgctgatg tcctgcccaa accgcaccgg aaaggcgatg
    21301 gctttatcga gtgcggtaat ggtcaggtgg tgacttggtg tatcggtcac ctgcttgagc
    21361 aggcgcagcc agacgcctac gacagccgct atgcgcgctg gaatcttgcg gatttgccga
    21421 ttgtcccgga aaagtggcaa ttacagccac gaccctccgt gaccaaacaa cttaacgtca
    21481 tcaaacgctt cctgcatgaa gccagcgaaa tcgttcacgc cggggacccg gatcgtgaag
    21541 ggcaattgct ggtggatgaa gtgctggact atctgcaact ggcaccggaa aagcgccagc
    21601 aggtgcagcg ttgcttgata aacgacctga acccgcaggc ggttgagcgg gcgatcgacc
    21661 gtcttcgttc caacagtgag tttgtgccat tgtgcgtttc tgcgctggcg cgagcgcgtg
    21721 ccgactggct gtacggcatc aatatgaccc gtgcgtatac cattctcggt cgcaatgccg
    21781 gttatcaggg cgtactttcc gtgggacgcg tgcagacacc cgtgctcggg ctggtggtgc
    21841 gccgcgatga agagattgaa aacttcgtgg cgaaagactt ctttgaagtc aaagcacata
    21901 tcgtgacacc tgccgatgag cggtttaccg ctatctggca accgagcgaa gcgtgtgaac
    21961 cgtaccagga tgaagaaggg cgcttgttac atcgtccact ggcggagcat gtggttaacc
    22021 gcattagtgg tcaaccggct attgtcacca gctataacga taaacgggaa tcagaatccg
    22081 cgccgctgcc gttttcgctt tcggcgttgc agattgaagc ggcaaaacgc ttcggtctga
    22141 gcgcgcagaa cgtgcttgat atctgccaga agctgtacga aacgcacaag ctaatcactt
    22201 atccgcgttc cgattgtcgc tatttgccag aagaacattt tgccggacgc cacgcggtga
    22261 tgaatgccat cagcgttcat gcaccggatc tgttgccgca gccagtggta gatccagata
    22321 tacgcaaccg ctgttgggat gacaaaaagg tcgatgcgca ccacgccatc atcccgaccg
    22381 cacggagttc tgcgatcaac ctgacggaga acgaagcgaa ggtctataac ctgattgccc
    22441 gtcaatacct gatgcagttc tgcccggatg cggtgttccg caagtgtgtt atcgaactgg
    22501 acattgccaa aggcaaattt gtcgctaaag cccgttttct tgctgaagca ggctggcgca
    22561 cgctgttagg cagcaaagag cgcgatgaag aaaacgacgg cacgccattg cctgtggtgg
    22621 cgaaaggcga tgagttgctg tgtgaaaaag gtgaagtggt agagcggcaa acccagccgc
    22681 cgcgccattt taccgatgca acactgcttt cggcgatgac cgggatcgcg cgctttgtgc
    22741 aggacaaaga tctgaaaaag gtccttcgtg cgaccgatgg tctggggaca gaagcaaccc
    22801 gtgccgggat tattgaactg ctgttcaagc gtggttttct gaccaaaaaa gggcgctata
    22861 tccactccac cgacgccgga aaagcgttat tccattcgct gccagaaatg gcgacgcgac
    22921 cggacatgac cgcgcactgg gaatcggtgc tgacgcaaat cagcgaaaag cagtgtcgct
    22981 atcaggactt tatgcagccg ctggtgggga cgctatatca gcttattgat caagccaaac
    23041 gtacgccggt gcggcagttt cgcggcattg tggctccggg cagtggtggc agtgctgata
    23101 agaaaaaggc tgcaccgcgt aaacgtagtg cgaaaaaaag tccgccagca gatgaagccg
    23161 gaagcggggc gatagcgtaa gcgagcgaat ctttcgtgct attcgagtca tattctgaaa
    23221 tatccagcgg atcaagaaag ttcgttggat atttttttgc atggataaaa ttatcgcctc
    23281 taaagtatgt aataacaggg aatgtgatga aaaaagtttt attgcaaaac catcctggga
    23341 gcgagaagta ttcttttaat ggctggggaa tatttaatag taattttgaa cgggtgatta
    23401 aagaaaataa ggccatgctg ctttgtaagt gggggtttta tttaacatgt gttgtcgctg
    23461 taatgtttgt attcgcagcg ataacatcca acggtttgaa tgaaagaggc ctgattaccg
    23521 cgggatgctc ttttctttat ctattaatta tgatggggct tattgttcgg gccggtttta
    23581 aagcaaaaaa agaacaactg cattattatc aggctaaagg tattgagccg ctcagtatcg
    23641 agaagttaca ggcgctacaa ttgatcgcac cttatcgatt ctatcataag caatggtctg
    23701 aaacgctaga gttctggccg cgaaagcctg aacctggcaa agataccttc caatatcatg
    23761 tgcttccttt tgactcgatc gatatcatca gtaaaagacg cgagtcttta gagaatcaat
    23821 ggggtatcga agatagcgaa agttattgtg ccttaatgga gcattttctt tctggcgacc
    23881 atggagccaa tacctttaaa gcaaacatgg aggaagcccc agagcaggtt atcgccttgt
    23941 tgaataaatt tgctgttttt ccctcagact atatctctga ttgcgctaat aatcgctccg
    24001 gtaaatcctc ggcgaagcta atatgggcgg cggaattatc atggatgatc tcgatatcaa
    24061 gcacagcttt tcaaaacggg acaattgaag aagaactggc ctggcattat ataatgcttg
    24121 cttctcgaaa ggcgcacgag ttgttcgaaa gcgaagaaga ttatcaaaaa aatagtctaa
    24181 tgggatttct ttactggcat atctgctgct atcgcagaaa gttaacggat gctgaacttg
    24241 aagcgtgtta tcgttacgac aagcagtttt gggagcacta cagtaagaaa tgccgttggc
    24301 ccataagaaa tgttccctgg ggagcatcat ccgttaaata ctcataaacg cctgaaattt
    24361 tgccgggggc gctgcgcttg accggcctac attagagcgt aactgcaatg aattgtgccc
    24421 atctgtaggc ctgataagcg tagcgccatc aggcatttac aacttaaatc acaccctgcg
    24481 ccagcatcgc atcggcaacc ttcacaaaac cggcaatgtt cgcgccctgc acgtagttgg
    24541 tttgctcacc ttcaccacca tgctcaacac aggcatggtg gatatccagc atgatgtgat
    24601 gcaaacgtgc gtcaactttc tcggctttcc agcccaggcg cgcagcgttt tgtgccattt
    24661 ccaggcccga cgttgctaca ccgccagcat tagccgcttt acccggtgca aacagtacgc
    24721 ctgcctgctg gaacagttca gtcgcttcga tggtggtcgg catatatgcc ccttcagcga
    24781 cggctttaac gccattggcg ataagctgat gcgcggcgtc aacatccagt tcgttctggg
    24841 tggcgcaagg cagggcgata tcaaccggta cagaccacgg ctgttggcct tcgagataga
    24901 ccagaccaaa ttctttggcg taatctgccg ctcgaccatc gcggctggct ttgatttcga
    24961 taagacgtgc cagtttctct ttcgtgaatc cgctttcatc aactacagtg ccgctggagt
    25021 ctgacgcagt gatcacacga gcaccaaatt ccatcgcttt ttcgatagcg tactgggcga
    25081 cgttgccgga gccagaaacg gaaacgctca tcccttcaaa acccataccg tggcgtttta
    25141 acattgcttc tgtgaaataa accagaccgt agccggtagc ttccgggcga ataagactac
    25201 cgccaaatga aaggccctta ccggtgaaga cgcaggcggt attgttggag agctttttca
    25261 tcatccccgc cataaagccg acttcacgac caccaacgcc gatatcacct gccggaacgt
    25321 cggtatccgc gcccaggtgg cgatacagtt cagtcatcag cgtctggcaa aaacgcatca
    25381 ctttaccttc gctttttcct ttcggatcga aatcgctgcc gcctttacca ccgcccatcg
    25441 gcagggtagt cagggcattt ttgaaggttt gttcaaagcc gaggaatttg agaatggaaa
    25501 ggttaactga cggatggaag cgcataccgc ctttgtacgg gccgatggca gagctgaact
    25561 gcacacgcca tgcacggttg acctgtatct ggttgcgatc atcaacccat accacgcgaa
    25621 actggatcac gcgctccggt tcaaccagac gctccagta
//
LOCUS       DAZPCS010000041        25500 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZPCS010000041 DAZPCS010000000
VERSION     DAZPCS010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25500
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.041"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            160..663
                     /locus_tag="ACHUQ7_001703"
     CDS             160..663
                     /locus_tag="ACHUQ7_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFR1135152.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(743..994)
                     /gene="yecJ"
                     /locus_tag="ACHUQ7_001704"
     CDS             complement(743..994)
                     /gene="yecJ"
                     /locus_tag="ACHUQ7_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFR1135153.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(1109..1195)
                     /gene="azuC"
                     /locus_tag="ACHUQ7_001705"
     CDS             complement(1109..1195)
                     /gene="azuC"
                     /locus_tag="ACHUQ7_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFR1135154.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            1458..1781
                     /locus_tag="ACHUQ7_001706"
     CDS             1458..1781
                     /locus_tag="ACHUQ7_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFR1135155.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            1952..2449
                     /gene="ftnA"
                     /locus_tag="ACHUQ7_001707"
     CDS             1952..2449
                     /gene="ftnA"
                     /locus_tag="ACHUQ7_001707"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFR1135156.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(2486..2725)
                     /locus_tag="ACHUQ7_001708"
     CDS             complement(2486..2725)
                     /locus_tag="ACHUQ7_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFR1135157.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGVTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            2915..4126
                     /gene="tyrP"
                     /locus_tag="ACHUQ7_001709"
     CDS             2915..4126
                     /gene="tyrP"
                     /locus_tag="ACHUQ7_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFR1135158.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(4188..4853)
                     /gene="yecA"
                     /locus_tag="ACHUQ7_001710"
     CDS             complement(4188..4853)
                     /gene="yecA"
                     /locus_tag="ACHUQ7_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFR1135159.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(5049..5135)
                     /locus_tag="ACHUQ7_001711"
     tRNA            complement(5049..5135)
                     /locus_tag="ACHUQ7_001711"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5099..5101),aa:Leu,seq:taa)
     gene            complement(5148..5221)
                     /locus_tag="ACHUQ7_001712"
     tRNA            complement(5148..5221)
                     /locus_tag="ACHUQ7_001712"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5187..5189),aa:Cys,seq:gca)
     gene            complement(5276..5351)
                     /locus_tag="ACHUQ7_001713"
     tRNA            complement(5276..5351)
                     /locus_tag="ACHUQ7_001713"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5316..5318),aa:Gly,seq:gcc)
     gene            complement(5503..6051)
                     /gene="pgsA"
                     /locus_tag="ACHUQ7_001714"
     CDS             complement(5503..6051)
                     /gene="pgsA"
                     /locus_tag="ACHUQ7_001714"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFR1135160.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYVGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(6108..7940)
                     /gene="uvrC"
                     /locus_tag="ACHUQ7_001715"
     CDS             complement(6108..7940)
                     /gene="uvrC"
                     /locus_tag="ACHUQ7_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFR1135161.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(7937..8593)
                     /gene="uvrY"
                     /locus_tag="ACHUQ7_001716"
     CDS             complement(7937..8593)
                     /gene="uvrY"
                     /locus_tag="ACHUQ7_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFR1135162.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            8735..8806
                     /locus_tag="ACHUQ7_001717"
                     /pseudo
     CDS             8735..8806
                     /locus_tag="ACHUQ7_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302039.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            8889..9065
                     /gene="yecU"
                     /locus_tag="ACHUQ7_001718"
     CDS             8889..9065
                     /gene="yecU"
                     /locus_tag="ACHUQ7_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFR1135163.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTT
                     NFHCNMTRKINEYA"
     gene            9052..9276
                     /gene="yecF"
                     /locus_tag="ACHUQ7_001719"
     CDS             9052..9276
                     /gene="yecF"
                     /locus_tag="ACHUQ7_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFR1135164.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(9309..9929)
                     /gene="sdiA"
                     /locus_tag="ACHUQ7_001720"
                     /pseudo
     CDS             complement(9309..9929)
                     /gene="sdiA"
                     /locus_tag="ACHUQ7_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(10159..10911)
                     /gene="tcyN"
                     /locus_tag="ACHUQ7_001721"
     CDS             complement(10159..10911)
                     /gene="tcyN"
                     /locus_tag="ACHUQ7_001721"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFR1135165.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(10908..11576)
                     /gene="tcyL"
                     /locus_tag="ACHUQ7_001722"
     CDS             complement(10908..11576)
                     /gene="tcyL"
                     /locus_tag="ACHUQ7_001722"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFR1135166.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(11591..12577)
                     /gene="dcyD"
                     /locus_tag="ACHUQ7_001723"
     CDS             complement(11591..12577)
                     /gene="dcyD"
                     /locus_tag="ACHUQ7_001723"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFR1135167.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDEVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(12682..13482)
                     /gene="tcyJ"
                     /locus_tag="ACHUQ7_001724"
     CDS             complement(12682..13482)
                     /gene="tcyJ"
                     /locus_tag="ACHUQ7_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR1135168.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKESGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(13570..14121)
                     /gene="fliZ"
                     /locus_tag="ACHUQ7_001725"
     CDS             complement(13570..14121)
                     /gene="fliZ"
                     /locus_tag="ACHUQ7_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFR1135169.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(14167..14886)
                     /gene="fliA"
                     /locus_tag="ACHUQ7_001726"
                     /pseudo
     CDS             complement(14167..14886)
                     /gene="fliA"
                     /locus_tag="ACHUQ7_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
     gene            complement(15051..16703)
                     /gene="fliC"
                     /locus_tag="ACHUQ7_001727"
     CDS             complement(15051..16703)
                     /gene="fliC"
                     /locus_tag="ACHUQ7_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HFR1135170.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASAMNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTAGY
                     SVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            16969..18375
                     /gene="fliD"
                     /locus_tag="ACHUQ7_001728"
     CDS             16969..18375
                     /gene="fliD"
                     /locus_tag="ACHUQ7_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFR1135171.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGDDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKVQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            18400..18810
                     /gene="fliS"
                     /locus_tag="ACHUQ7_001729"
     CDS             18400..18810
                     /gene="fliS"
                     /locus_tag="ACHUQ7_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFR1135172.1"
                     /translation="MYAAKGTQAYAQIGVESTVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSSIQDPV"
     gene            18810..19175
                     /gene="fliT"
                     /locus_tag="ACHUQ7_001730"
     CDS             18810..19175
                     /gene="fliT"
                     /locus_tag="ACHUQ7_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFR1135173.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            19253..20740
                     /gene="amyA"
                     /locus_tag="ACHUQ7_001731"
     CDS             19253..20740
                     /gene="amyA"
                     /locus_tag="ACHUQ7_001731"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFR1135174.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAECWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(20774..21187)
                     /gene="yedD"
                     /locus_tag="ACHUQ7_001732"
     CDS             complement(20774..21187)
                     /gene="yedD"
                     /locus_tag="ACHUQ7_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFR1135175.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRGIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            21374..22579
                     /gene="yedE"
                     /locus_tag="ACHUQ7_001733"
     CDS             21374..22579
                     /gene="yedE"
                     /locus_tag="ACHUQ7_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFR1135176.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRE
                     APQTAKEIA"
     gene            22576..22809
                     /gene="yedF"
                     /locus_tag="ACHUQ7_001734"
     CDS             22576..22809
                     /gene="yedF"
                     /locus_tag="ACHUQ7_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFR1135177.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            22916..23586
                     /locus_tag="ACHUQ7_001735"
                     /pseudo
     CDS             22916..23586
                     /locus_tag="ACHUQ7_001735"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(23626..24834)
                     /locus_tag="ACHUQ7_001736"
                     /pseudo
     CDS             complement(23626..24834)
                     /locus_tag="ACHUQ7_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
     gene            24842..25268
                     /gene="tnpA"
                     /locus_tag="ACHUQ7_001737"
                     /pseudo
     CDS             24842..25268
                     /gene="tnpA"
                     /locus_tag="ACHUQ7_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT         6288 a         6540 c         6113 g         6559 t
ORIGIN      
        1 cttataaccg ttcagacgtt attcttattt cagatcatcg tcagaattga ctccacgatc
       61 acatttcgga ccggcagaaa ggaattattc tgcaaacagt aattatggtg ttttgattta
      121 tcttgcacct ctccacttct ggatataagg atattaggta tggcaaccgc tggaatgctt
      181 ctcaaactca actctcaaat gaaccgcgag ttttacgcat ccaatctcta ccttcacctg
      241 agtaactggt gttctgaaca gagtctgaac ggcaccgcca ctttccttcg cgcccaggca
      301 cagagtaatg tgacccaaat gatgcgcatg tttaacttta tgaagagtgt cggcgctacc
      361 cccatcgtta aagccattga tgttccaggt gaaaaactga actctctgga agaactgttc
      421 caaaaaacga tggaagaata cgagcaacgt tcaagcactc tggcgcagtt agccgatgaa
      481 gcgaaagaac tgaatgatga ttcaaccgtc aatttcctgc gcgatctgga aaaagaacag
      541 cagcatgatg gcctgttgct gcaaaccatt cttgatgaag tgcgcagtgc gaaacttgcg
      601 ggtatgtgcc ctgtgcagac cgaccaacat gttctgaatg tcgtgtcaca ccagctgcat
      661 tgatcataca tcggcgctaa tacattgcgc cgatgaagtt tttgagaaac cgctgcctca
      721 tctgtttgaa gcagcggttt ttttaatggg attcaccctg tggggtaaac ttgagttcaa
      781 tcagcgcgat ggctttttgg attgcccgca tggtgaccgg gtctgcggca gcgggatgat
      841 gagtaaagtc gatattcttc agctgactgg acattttttc acgaacttca acgggcgcga
      901 ttacatcgag aacatccaga atttgtttga taaccaactg gcaagctacc acatcagaaa
      961 ccagttcctg atcggcattc agcggctggg acatcgtaaa ctcctgatag cattttgaaa
     1021 gccgttatag tagcggcttc acatcttcag cgatagtcac atccaccgtc atcaggacac
     1081 aaaaaaacct gccggagcag gttttttgtt atcggaacat attgcctggc ggtacgtctt
     1141 tgaacgtctt gcaatagtta ttgaacatac ttttcaggat tttgcgcagt ttcatcgcgg
     1201 cactccgacc atttgttata caggtgttat tgtctttgcg cttataatat gacaaccatc
     1261 acaaaaatca atctttatgt gatacaaatc acataaatac ccctttaatg ttataaaaat
     1321 gataatcaaa aaacagcccc cctatttctg acacctacag atggcaagaa atagcgcctg
     1381 ccaggcgtct tttccggcca ttgtcgcagc gctgtaacgc gtaaaatagt gctttctctt
     1441 actcttctgg ctggaccatg agacttctga ttctgactct ttcattaata acgctggcgg
     1501 ggtgtacggt tactcgccag gcccatgtga gcgaagttga cgcggcaaca ggtattgtac
     1561 ggttggttta tgaccaggct tttttgcagc atgcccatac cgatcgttat gtcagtcgcg
     1621 ggatcgccga tcgcgcatgc cagcaggaag gctataccca cgcgatcccc tttggtcagc
     1681 cagtaggcaa ctgtagcctt tttgcgggtt ccttatgttt gaatacggaa ttcactttgt
     1741 catatcagtg ccatcattcc gccttccctg tctttctcta aatattttaa tcagcgaggg
     1801 gatcttcgct gattaaagaa atagcggtaa tgcgttttat tcccattcgt atttttaata
     1861 attgaagttt atattttacc ttttgcaaat aataaaataa caaattatag tgacgccaca
     1921 gcaacaaata taacctttgt ggagcactat catgctgaaa ccagaaatga ttgaaaaact
     1981 taatgagcag atgaacctgg aactgtactc ttcactgctt tatcagcaaa tgagcgcctg
     2041 gtgcagctat cataccttcg aaggtgctgc cgcgttcctg cgccgtcacg cccaggaaga
     2101 gatgacgcat atgcagcgtc tgtttgatta cctgactgat accggcaatt taccgcgtat
     2161 taataccgtt gaatctccgt ttgctgaata ttcctcactt gatgaattat tccaggaaac
     2221 ctataaacac gaacaattaa tcacccagaa aattaacgaa ctggctcatg ctgcaatgac
     2281 caatcaggac tacccaacat ttaatttcct gcagtggtat gtttctgagc agcatgaaga
     2341 agagaaactg ttcaaatcga ttattgataa attaagcctg gcaggcaaaa gcggcgaagg
     2401 tctgtatttt atcgacaaag aactctctac cctcgacaca caaaactaat gctctccggc
     2461 ggcaggtttc tgccgccgtt tcaaatcagt gacggcaaat cttactctga tcggtcgaaa
     2521 aaccttcttc tgaaggtata aatttgcctt ttgctgccag aaacgctacc agctctcccg
     2581 ctgtcacccc ttctgccgag cacgtgtgaa aacgtgcctg ttcaccaaaa cgcgctttaa
     2641 tcgcagcttc cagactggca tgcgtatatt gctcgcctga ttcaatcatc atatttaaca
     2701 cttcatgacc gtgaatagag tccatcgttc ctcctcaaaa aaagcctagc gtagcgatta
     2761 ccgcttatga agactttgcg ccagcgcagg actgaatgct tttattgtac atttatattt
     2821 acacaatatg taacgtcggt ttgacgaagc agccgttatg ccttaacctg cgccgcagat
     2881 atcactcata aagatcgtca ggacagaaga aagcgtgaaa aacagaaccc tgggaagtgt
     2941 ttttatcgtg gcgggaacca caattggcgc aggcatgctg gcaatgccgc tggctgcggc
     3001 cggtgttggt tttagcgtta cgttaatctt gttgattggg ctttgggcgt tgatgtgcta
     3061 cacggcgcta ttactgctgg aggtgtacca gcatgttccg gcagataccg gtctgggcac
     3121 gctggcaaaa cgctatctgg gacgctacgg tcaatggctg acgggcttca gtatgatgtt
     3181 cttaatgtat gctctgactg cagcatacat cagcggtgcc ggtgaattgt tggcctccag
     3241 catcagcgac tggacaggta tttcaatgtc ggcaaccgct ggcgtgctgt tgttcacttt
     3301 tgttgccggt ggcgtggttt gtgttggaac atcgctggtc gatttattta accgttttct
     3361 gttcagcgcc aaaattattt ttctggtggt gatgctggta ttgctgctac cgcatattca
     3421 caaagtgaat cttttaaccc tgccgttgca acaggggctg gcactgtctg caatcccggt
     3481 gatttttacg tcgtttggtt ttcacggtag cgtgccgagt attgtcagct atatggatgg
     3541 caacattcgt aagctacgct gggtgtttat aatcggtagt gccatccccc tggtggcata
     3601 tattttctgg caggtggcga cgcttggcag cattgattca acaaccttta tgggattgct
     3661 ggctaatcat gctggattaa acgggctgtt acaggcgtta cgcgaaatgg tggcctctcc
     3721 gcatgttgag ctggcggtgc atttatttgc tgatttagcc ctcgccacgt catttctcgg
     3781 cgttgcgtta ggcttatttg attatctggc tgatttgttt cagcgttcaa ataccgttgg
     3841 tggacgattg caaactggtg caattacctt tctgccgccg ttggcgtttg cactgtttta
     3901 tccacgagga tttgtgatgg cgctgggtta cgccggtgtg gcgctggcgg tactggcatt
     3961 gattatccct tcactattga cctggcaaag cagaaagcac aatcctcagg cgggttaccg
     4021 ggtcaaaggt ggtcgtccgg cgctggtggt ggtgtttctc tgtggtattg ctgtgattgg
     4081 cgtgcaattt ttgattgcgg cagggttgtt accagaagtg gggtgatcag atagcctcaa
     4141 attccttatt gggtgccaga attaacgctg acacccaatg tgacctctta atgcaggcag
     4201 cactgcttaa atttcttacc actgccgcac ggacaaggat cgttacgccc cggtttctct
     4261 tctgctttga tcggttgctg aacagctttt tcctgcggat gcgccatcca gtatgcatgt
     4321 aaatcaagcg ccgccagtcg aatggcatct acgctctctt caaacgcttc tggcgacatt
     4381 ttctctaccc gctcgaagtt ttcctcagta ccgtgcagcg caatcgcctc cagcgctggt
     4441 tttaaagaat cgggcaacgt tgaccagtca gaaagtgcca cgccccgcat atagccaaag
     4501 caccactcct caacaatcgt cagctcgctg ccatcaactt ctcgcaagcc gaataacggc
     4561 tcaaactgct ccgggaattc gttcagacgc tctgcggtat cggccatatg ttgaaaagcc
     4621 aggttcataa agcgcgtcat ctctttctct gacgcccaac gcggcacata gtcagcccca
     4681 ccccacacgg caaccagcca ctgttctggt tcaatctctt gcggagaact caacaccgcc
     4741 gtcaataaac cgtccagctc cgccacatca aggatggcgt ggtcagtgtt gtatttggtc
     4801 agaatatcgt ccagccattc caactcactt tcgtttaacg gtcccgtttt catacgcttt
     4861 tccttgtgga tctcaactcg ccagcaccta tcttacatgc cggtccgtat cagagatact
     4921 ttttgagtgg ctttgccggt gattaaaaat taaggagggt gtaacgacaa gttgcaggca
     4981 caaaaaaacc acccgaaggt ggtttcacga cactgcttat tactttgatt ttattcttat
     5041 ctttcccatg gtacccggag cgggacttga acccgcacag cgcgaacgcc gagggatttt
     5101 aaatcccttg tgtctaccga ttccaccatc cgggctcggg aagaaagtgg aggcgcgttc
     5161 cggagtcgaa ccggactaga cggatttgca atccgctaca taaccgcttt gttaacgcgc
     5221 caaattcttc aggcctttca gccagacatc cgcttgacgc cgatgtcttt taaactggag
     5281 cgggaaacga gactcgaact cgagacctcg accttggcaa ggtcgtgctc taccaactga
     5341 gctattcccg cattcatcaa gcaatcagtt aatcacttga ttttattatc gtctggcaat
     5401 cagtgccgcc gttcgatgcg ttgcattcta cttacctggc gcgatgagtc aacgatattt
     5461 ttcaccactt ttgatcgttt gctgaaaatt acgccgaaac gatcactgat caagcaaatc
     5521 tgcacgcgca gcgctcaaat attgcaacat tgaccacaga gtcagtaccg cagccacaaa
     5581 gaaaagtgca ataccgacgt actcaaccca aatgttcgga cgccacagca gccaggccaa
     5641 cgccaccatc tgggcagtgg ttttcacttt cccaatccag gagacggcca cgctactgcg
     5701 tttacccaac tccgccatcc attcgcgtag cgcagaaata ataatttcac gggcgatcat
     5761 cgttgccgcc ggtaatgtca cccaccagct gtgataatgc tcggttacca gcaccatggc
     5821 gatagccacg agaactttat ctgccacagg gtcaaggaaa gcaccaaacc gggtactctg
     5881 gttccagcgg cgtgccagaa aaccatcgaa ccagtcagtc accgccgcga cgcagaaaat
     5941 gagcgcggcg gcaaacggcg accaggtgac aggcagataa aagaccaata caaagaatgg
     6001 gataaggatg acacggaaca gtgtaagcaa cgtagggata ttaaattgca taatgacggg
     6061 taactatctg ttgtcagtaa gattacccct atgttgctac agagacatca atgtttcaac
     6121 gaccagaaga tcttttctgc cagaccttgc gaaatacccg gcacttttgc aatttcctcg
     6181 acgctggcgt tacgtaaacc ttgcaaaccg cccatatatt tcaacaacat ttgccgacgt
     6241 tttggcccga cgccttcaat ggtttccagg gaactggtat ttttgacctt cgcccgtttt
     6301 ttacggtgcc cgccaatcgc gtgatcatgt gattcatcgc gaatatgctg gataacatgc
     6361 agcgcgggtg aatctggcgg caaactaaat ccctcacctt ccggctcaaa gaacagcgtt
     6421 tccagtccag ccttacgatc tgctcctttg gcaacgccaa gtagcagcgg atgattttta
     6481 tcccatgaga catccagttc ggcgaagaca tttttcgcct gcgcaagctg gcctttgccg
     6541 ccgtcgataa ggatcacatc cgggatctta ctgtcgtcaa tggctttacc ataacgccga
     6601 cgcagcacct gattcatcgc cgcataatca tcgcccggcg tgatgcctgt aatgttatag
     6661 cgccgatact ccgcacgcag cgggccgtta gcatcaaaca ccacacagga agcgacggtt
     6721 tgttcgccca tggtatggct gatgtcaaag cactccatcc gcttcacttc cggcaatttc
     6781 aacacgctgg caagcgcggt cagtcgctgg tgaacggtag attgctgcga aagtttgctg
     6841 gttaaggccg tcgccgcatt ggtgcgcgcg agtttcagat aacgcgccct atcgccgcga
     6901 ggtttggttt gaacattaat cttgcgtccc gccagttctg aaagggaatc ggcgagcagc
     6961 gttttatcgc taagattaaa atcgagcagg atctcacccg gtaaggtgcg catctggctg
     7021 ccttgtaaat agaactgacc tacgaaggtt tctaccacct cgctcagttc cgtaccgcca
     7081 ggcactttcg ggaaatagct gcggctgccg agcactttgc cctgacgaat gaacaatacg
     7141 tggacacaag ccatgcccgc atcgaacgcc acaccaataa cgtcaaggtc gtcgccggta
     7201 ttggaaacaa actgtttttc ggtgacgcgt cgcaccgcct gaatttggtc gcgaatacat
     7261 gccgcttctt caaactccag attctggctg gcagtttcca tacggctaat gagttgcgta
     7321 agcacctgat catctttgcc agacaaaaac aggcgcacat actcgacctg ctgagcgtat
     7381 tcttcttcac tcaccagtcc ttcaacgcac ggtcccagac agcgccctat ctggtattgc
     7441 agacacggac gcgagcgatt gcgataaaca ctattttcgc actggcgaat ggggaaaatc
     7501 ttttgcagta gcgccagtgt ttcacgtacg gcatagccat tcgggaacgg gccgaaatat
     7561 tcacctttgg catgctttgc accacgatgc atcgccagac gcgggtgggt atcgccactc
     7621 aggaagataa aaggataaga tttatcatcg cgtagcaaaa cgttgtaacg cggctgatag
     7681 agtttgatgt agttgtgttc cagcaacagc gcttcggttt ctgtatgcgt aaccgttaca
     7741 tcaatttgct ggatctgggc gaccagcgct tcggttttgc gcgaagcgag gttgctgcgg
     7801 aaatagctgg aaagccgttt tttcaggtct ttcgctttgc cgacatagat aaccgtacca
     7861 ccagcatcgt acatgcgata aacgcctggc tggctggtta cggtttttaa aaacgctttt
     7921 gcgtcaaact gatcactcac tgacttgata atgtctccgc attacacaga ccatggcgaa
     7981 ttgccaggtg agtcagctca acatcgccat gaatgtttag tttactgaac atacgatagc
     8041 ggtagctgtt caccgttttc ggactgagat tgagctgttc tgagatctca ttgaccttct
     8101 ggcccttagt gatcatcagc ataatctgca attcacgttc agacaaactg gcaaatgggc
     8161 tttctgtttt ttctggttcg atctggctta acgccatttg ttgagcgata tcagaagcaa
     8221 tgtaacgctg ccctgaatag acagaacgaa tcgcactcac gacttcctgc ggagccgcgc
     8281 ctttgctgag gtagccagca gcacctgcct gcatgacttt cgctggtaaa gggttttctg
     8341 tatggaccgt aagcatgatg attttaacat cagctgtgga acgcgcgatt ttacgcgtcg
     8401 cctcaagacc gccaatgccc ggcatactca tgtccattag caccacgtca acggcatttg
     8461 tccggcacca cttaacggcg tcttcaccgc acgatgcctc tccgacgact tttataccct
     8521 taatatcttc cagaatgcgt cgtatccctg cgcgcaccag ttcgtggtca tcaacaagta
     8581 gaacgttgat caaaggaata tctccagaaa tagggataac gctactgata gttagtcatt
     8641 cgtatattag cggtttttat tgcaactttg aaacgttaaa aatggtgggt attcgatttt
     8701 tctctcgttt ttgtcaattc gattgtccac agttatggtc acgcccgtca ccaacggctt
     8761 accggatcgt ttcacagtaa gccctacgat gaaatgtgac aaaaattgac tttattcagc
     8821 aaaaatgaaa atcagcccgc tgaatatgta acattaatta acccgaacaa tacgggcaaa
     8881 aaacattcat gataaaaata tttattggtc attatattaa cgtattttat agcactgccg
     8941 atatcacgct caaaaaacaa ccactgctat ttttagcaaa gcttatggta tactccgccg
     9001 ccttaacatt tttcaccaca aattttcatt gcaacatgac gaggaaaata aatgagtacg
     9061 cctgattttt ctactgccga gaataatcaa gaactggcaa atgaagtctc ctgcctgaaa
     9121 gcgatgctga cgctgatgct gcaggcgatg ggacaagctg acgcgggccg cgtgatgtta
     9181 aagatggaaa aacagcttgc gctgatcgaa gacgaaaccc aggctgcagt attttccaaa
     9241 acggttaagc aaattaaaca agcctaccgt cagtaatata aaaccggctg agagcgtgcc
     9301 tttcagccgg ttattgacaa aatcatcgct atctctgctg atgtcttccc ttccgccgtc
     9361 catttcagaa tttctttttc gcgcttgctg aaattcatct ctggcgtcat cactatttca
     9421 tcatttaaac gcatcagagc catcagactt tcgcgcacca gtaactgcat ttttaattgc
     9481 agttcatcac taagaatggg tatttcgcgc gtgctgcaac gggaaaagga caaaaagccc
     9541 agcgcccgat ttggcagcat taaatactga gtgacaccgc ggcgtaaacc atgtgcgtgc
     9601 gcggcttccc ataatggctg tgcttcgctg aataagtcat cattccacat taaatggccc
     9661 tgactaaagt tttcagggtt cagcacagga tcaattgcga gaaaattttt tgcctgataa
     9721 taactaaccc acgactcagg gtaattggtg tagaaagcca ctttaggtcg cgtgaatggt
     9781 accgggtggc gaacacataa cgaatagtaa tcgtactcca gctgctgagc ctgaagctct
     9841 atttcatggt aggcctcttc tgcggcctcc atcctctgaa aacgcaacag catcgtgcga
     9901 cgccagctga aaaaatccgt atcctgcata gtaaaccgca acgcccctga gagtgagtat
     9961 catttataat gataattcaa actatcacat aaataagatt tatatataat ttatattatt
    10021 caggcaatga attacttttg caagccatcg cattctctta tgttattaat gagttatgct
    10081 gatttgttaa gcagttttat cacgcttgaa atggcgtccc gccccgacag gtgaatcgtc
    10141 ggggctgatt ttttcttatt attgcagcag aagcttctcg aggaactggc gggtgcgagg
    10201 ctgctgggga tcggcaaata acgcttttgc tgccccctgc tcgactatcc gcccctggtc
    10261 cataaagatc gcccggtccg caacatcccg ggcaaagctc atttcgtgtg tcacaatcac
    10321 catcgtgcgc ttttcctgcg ccagctggcg gatggtgttc aggacttcgc ccaccagctc
    10381 tggatccagc gccgacgttg gctcgtcaaa caaaatcacc tcaggacgca ttgccagtgc
    10441 acgcgcaatc gcaacacgct gctgttgacc gccagacaaa cgacgtggat agctggtttc
    10501 tttaccggcc agcccaactt tcgccagcag ctcgcgagcg cgcgccgtgg cctcttcttt
    10561 cggttcgcct ttgacgatca ccggcccttc aataatgttt tccagcaccg tacgatgcgg
    10621 aaacaaatta aagttctgga agacaaaccc gacgtgctga cgcaactggc gaatcagcga
    10681 tttttgctga cttaatgaac gtgcagtatc aatagtgata tcgccaacgg tgatcgttcc
    10741 cgcttccggt tgttccagca gatttatgct acgtagcaat gtggttttgc cggaaccact
    10801 cggaccgata attgccacca cttcgccagg ctttacctcg aggtcgatac cgtgcagcac
    10861 cgtctgaccg tggaattttt tcaccaggtt cttaacttca atggcactca ttttggttct
    10921 ctctcctggc gattaagttg attctcaaaa tggttctgca acgtcgataa cacagtcgcc
    10981 atgatccagt agatgagcga agccgccagg tacatggtga acacctccag cgtgcgcgag
    11041 gtaatcaact gcgcctgacg gaacagctcc ggcacctgga ttgtcgcggc cagcgacgta
    11101 tcttttacca gactgataaa gctgttcgac agcggcggca acgccacccg cgccgcctgt
    11161 ggcaaaatcg cgcgacgcat ggtctgccac ggcgtcatac caatactggc agcggcttcc
    11221 cactgacctt tatcaataga agaaatggcg gcgcgcagcg tttcggcggc ataggcggca
    11281 gtgttcagcg acagaccaat cattgctgac ggaatgggat ctaattcgat accaaactgc
    11341 ggcaggccgt aatagatcat aaacagttgt gcgatgagcg gcgtaccacg aaaaatagag
    11401 atataaaaac gcgccagcca gcgcacgggc cagattggcg acaggcgcat cagcgcgaga
    11461 ataaacccga gtagtaagcc aaaaaacatg ccgccgatac tgagttgcag ggtataccca
    11521 gcccctttca acaggaacgg caaggaatca ataaccagtt gtatactttc ttgcatgagc
    11581 gttcccggag ctaaacgtgg ggatgatagg cgaacagcgc aggcgcgccg ccggtatgaa
    11641 taaacagaat cggcccttca tctttgaagc gtttctgact gataccgtca atcagccccg
    11701 ccatcgcttt tccggtatac acaggatcaa gcagaatgcc ttccagacgc gccagcaatt
    11761 tcactgcttc catgccttcg tcgttcggca cgccgtagcc aggtgcaaaa tagtcatccc
    11821 agagtaaaat ttccgctgat gcggtcagct ccagttcttt cgcaatcgcc tgttgtaggt
    11881 taaccacttt cggcaattga tcggcaacgg aacgcgacac ggtcacgcca atcagttcgc
    11941 tttcaggcat caggtgttcc agcccaacag ccagtccggc gtgagttccg gcactgcccg
    12001 atgcgactac caccgacgaa atattaaccg ccccttcaca ctgttgcgcg atttccagcg
    12061 cactctccac ataacctagc gcgccaagag cattagaacc gccaaccgga atgacatacg
    12121 ggcgaaagcc ttgtgcttcg actcgcgtcg ccagttcttc cagttgggca ttgggatcgg
    12181 tcagtgcgtc gcacatttca atctgggtat tgaacagatc cagcaacaaa cgattgccgt
    12241 tggttaaata gttttctgcg gttgtgccaa taggattttc cagcagcgcc acgcagtgca
    12301 gaccgagttt cgccgcgact gcggcagtct ggcgcacatg gttagactgg atcgcgccgg
    12361 cagtaatcag cgtatcggca ccttcgcgca gggcatctgc cgcgagaaat tccagcttac
    12421 gtaatttatt gccgcccatt gccatggggg tgacctcatc ccgtttgatg aaaatttccc
    12481 gtccaagata atcagaaaag cgcggcagat attcgagcgg cgttggcgcg ccgataaact
    12541 ccaaccgtgg aaaacgggtt aaattatgca gtggcataac agcctccgat gtgtgttgtt
    12601 gtgattttct tattatgcac gctgaaaacg cgtaaataaa aaaggcgcta gtgaaagcgc
    12661 ccttttttgt cattatgctg attatttggt cacatcagca ccaaaccatt tttcggaaag
    12721 agcttgcaga gtgccgtctt tttgcatttc cgcaattgca tcattcaccg ctttcagcag
    12781 gtcttcattt cctttacgca gcgccacgcc agactcctga cgggagaatg cttcaccggt
    12841 tactgccagc gtatcgttgg ttttcttcac cagatccagc gccgccagac gatcaacgag
    12901 gatcgcatcg atacgcccta cgcgcagatc ctgatatttg gtcgggtcat catcataggt
    12961 acgcacatcg acaccctgaa cattctgccg cagccactct tcatagttgg tgcccagacc
    13021 gacacccact tttttgcctt tcagatcagc agccgtttta atggtgcctt cgttaccttt
    13081 tttcaccagc gcctgaatac cggaaatggt gtaaggggta gagaaatcgt attttttctt
    13141 gcgctcgtca gaaatggtga cctgattaat caccacatca atgcgtttag agtccagcga
    13201 cgccagcata ccgtcccatt tggtcggttt tagtgacgcc tcaacgccaa gatgttttgc
    13261 cagctgttgg gcaaattcca cttcaaaacc ggttaatttg ccgtcatccc cctgaaaact
    13321 gaacggcgga taagtgcctt ccagccctac cagcagcgtg ccgctctctt tgactttatt
    13381 aagcagacct tcatctgcaa aacttttaac gctcatgccc gcaaccagcg ccacggccat
    13441 cacacccatc aatgcctgac gtcccagatg tgctaatttc atattcaccc cgaatgttgt
    13501 tatgtctgtt tgcagtgtag agccattgtt tgtaaacaca aaaacaactc cgctacatct
    13561 tattcttatt taatatatat cagaagcagg caggctggag gatttctgca caagtccggt
    13621 acaggtttgg cgctgatagt gttgatactt ccgtaatgcg atgcggtaat tgttggtgct
    13681 ggtggtgggc agccacgggg cgaggatttc atcaaggaaa ccgtcctgca gctgatcgag
    13741 ggaaatattt tgctcatgta gatgattacc caaacggcgc agacgaacga catattcacg
    13801 caccgtacct gggctcattt cagtttgctc aaaaagaaat ttcttaaagc cgataatatc
    13861 gaaaaaatca ctctgcgttt tgcaatgtaa atcaccgcaa aatcggcaca atgccgccca
    13921 tgattgttgt tccgtttgcc agcctttttc atcaagcagc gtgtccaggc gggaaatctc
    13981 gattttattc actattttgc cgtcgcgaac taaggtaatg cgatcgagta atttacggca
    14041 atgcgcgcaa tgggtctggc tgtgtttaaa gtctttaaga tagcggctta atggccgtct
    14101 tttcaggtgc tgcaccatca ttaagaactc ctggtagtca aagttaaagt gcggcattta
    14161 ctgacgttat aacttaccca gtttagtgcg taaccgttta atgtcctggc tgtgtaactg
    14221 actgacccgc gattccccga cctccagcac cgcgccaatc tctttgagat tcagctcttc
    14281 ctggtaatag agggttaata ccagtttttc gcgctccggc aacgtttcga tggcttccat
    14341 cacccgctgg cgcagattac tgtccagcag ttgttgtagc gggttttctc gctgatgatc
    14401 atcagtaacc agttcgatgc tatcgccgtg ctcttcacgc cactcatcgt aggagaagag
    14461 ctggctgtta ttggtgtcga gcagcatttg acgataatcg gcaatatcga tccctaaacg
    14521 ttccgctacc tcagtttccg tggcgttgcg gccaagttcc tgctccagtt gccctattgc
    14581 ctgtgccact tcacgcgcgt tgcgtcgcac gctgcgcggc acccagtcac ggctgcgaag
    14641 ttcatccagc atagcgccac ggatacgcta cactgcgtaa gttgtaaatg ccgttccttg
    14701 tagggcgtca tagcgttcga cagcattaag taacccaatg ccgcccgcct gtagcagatc
    14761 gtcaagttcc acgctcgcgg gcagtcgaac ctgcaggcgc aatgcttcgt gacgcaccag
    14821 cgggacataa cgctgccaca gcgagtgttt atccattaca ccttcagcgg tatagagtga
    14881 attcacgata aacagccctg cgttatatga gttatcggca tgattatccg tttctgcagg
    14941 gtttttaatc ggacgattag tgggtgaaat gaggggttat ttgggggtta cgcaaaaagc
    15001 cgaatgtaaa aaaccccgcc ggtggcgggg tttgagaggc attcagacga ttaaccctgc
    15061 agcagagaca gaacctgctg cggtacctgg ttggcttttg ccagcacgga gttaccggcc
    15121 tgctggataa tctgcgcttt cgacatgttg gacacttcgg tcgcatagtc ggcgtcctga
    15181 atacgggact gcgcttcaga caggttggta gtggtgttgt tcaggttggt gactgcggaa
    15241 tccaaacggt tttgcaccgc acccagggag gagcggaatt tgtcgatgga gctgatggct
    15301 tcatccagag ctttcagggg atcggctgtc gtagctgctt ttgtttcagc atctgtcgtc
    15361 aatttaccat cagcatcttt ataaactgcc ttaccgctgc cgttagtcac tgaaccatca
    15421 tcctgtaggt aaaaatctac agtatcagat gtggtcaacg cctcgttgtt atcaacataa
    15481 accgaatcaa caccttttgt tacagcgcca gttgtgccgt tgacgctata acctgcagta
    15541 gtgaaatcaa cattgtttcc cgaggttgat acagctttat cgaaagctgc ctgatcaact
    15601 tttgcacctg ttactgaata agtaatagtg tccggcgtag tactattacc ggtaaaggag
    15661 atagaggtac cgccaatgtc aatggttgtc gctttggtat tattggctgc aagtgtggac
    15721 agactagcct cagtcaaaga agcaccagag ccgtttttcg ttaagcgccc agttgtatca
    15781 atgtagagtt tatctccatt gcttgccgta attttaccat cactgctgat ctgaacatcc
    15841 tgtgcagaac catcgatagt aatagtgccc ttagcggtat caccaactcc cggggtcaaa
    15901 tatttctgaa catcggcagc tgaagccgtt gtggtatcaa aagagtatga cttacttgca
    15961 ctgtcatact tgtaattcat tgcactagcc gccgttccga agccgttatt aatggttgcc
    16021 tgaacagtat caccatcact aactccagcc agcaaatcag acgctttagc agcatcgtaa
    16081 cccgcactca cagtatattt gttgccgacc actgttgcat tagcagctac caagtcagct
    16141 ttagatgcag cagtattagc cacagccccg ccaccattca cattaaaccc attcagcccc
    16201 agcgtatcag agtcaatttt cttcagatca atagtgatag tctggccgtc attcgcacca
    16261 acctgaattt tcatcgaacc gtcttttgcc agtacgttca cgccgttgaa ctgggtctga
    16321 ccggatacgc ggtcaatttc gtcgagacgg gatttgattt cgtcctgaat ggagtccaga
    16381 tccgaatcag agttagtccc ggtagaagcc tgaaccgtca gctcacggat acgctgtaag
    16441 ttgttgttga tttcggacag cgcgccttca gtggtctgtg caacagaaat accgtcgttg
    16501 gcgttacgtg cagcctgagt caggccttta atgttagaag taaaacggtt agcaatcgcc
    16561 tgacccgcgg cgtcatcctt cgcgctgtta atacgcaagc cagaagacag acgctcgata
    16621 gaactcgaca gcgcagactg gttcttgttg atattatttt gagtgatcag cgagaggctg
    16681 ttggtattaa tgacttgtgc catgattcgt tatcctatat tgcaagtcgt tgattacgtt
    16741 ttgggtttcc acccgtcggc tcaatcgccg tcaaccctgt tatcgtctgt cgtaaaacaa
    16801 cctttagaat ttttttcaca aacagccatt ttttgttagt cgacgaaata ctcttttctc
    16861 tgccccttat tcccgctatt aaaaaaaaca attaaacgta aactttgcgc aattcaggcc
    16921 gataaccccg gtattcgttt tacgtgtcga aagataaaag gaaatcgcat ggcaagtatt
    16981 tcatcgctgg gagtcgggtc aggtctggat ttaagttcaa tccttgatag cctcaccgcc
    17041 gcgcaaaaag cgacgctaac ccctatttca aatcagcaat cgtcgtttac cgctaaactt
    17101 agcgcctacg gtacgctgaa aagcgcgctg acgactttcc agaccgccaa tactgcattg
    17161 tctaaagccg atcttttttc cgccaccagc accaccagca gcaccaccgc gttcagtgcc
    17221 accaccgcgg gtaacgccat cgccgggaaa tacaccatca gcgtcaccca tctggcgcag
    17281 gcgcaaaccc tgaccacgcg caccaccaga gatgatacga aaacggcgat cgccaccagc
    17341 gacagcaaac tcaccattca acaaggcgac gacaaagatc cgattaccat tgatatcagc
    17401 gcggctaact catcgttaag cgggatccgt gatgccatca acaacgcaaa agcgggcgta
    17461 agtgcgagca tcattaacgt gggtaacggt gaatatcgtc tgtcagtcac atcaaatgac
    17521 accggccttg ataatgcgat gacactctcg gtcagcggtg atgatgcgct acaaagtttt
    17581 atgggctatg acgccagtgc cagcagcaac ggtatggagg tctcggtcgc cgcccagaat
    17641 gcgcagctga cggtcaacaa cgtcgccatc gagaacagca gcaacactat cagcgacgcg
    17701 ctggaaaaca tcactctgaa cctgaacgat gtcaccacgg gcaaccagac gctaaccatc
    17761 actcaggaca cctccaaagt gcaaacggcg attaaagact gggtgaatgc ctataactcg
    17821 ctaatagata ccttcagcag cctgaccaaa tacaccgccg tagatgcggg agctgatagc
    17881 cagagttcta gcaatggcgc actgctcggc gactccacgc tgcggacgat tcagacgcag
    17941 ttgaagtcga tgctgagtaa taccgtcagt tcttccagct ataaaacgct ggcgcagatt
    18001 ggtatcacga ccgatcccag cgatggcaaa ctggaactgg atgccgacaa actcaccgct
    18061 gcactgaaaa aagatgccag cggcgtaggt gcattgattg ttggcgatgg taaaaaaacc
    18121 ggcatcacaa ccaccatcgg cagcaacctg accagttggc tttcgacaac gggcattatt
    18181 aaagccgcta ccgatggcgt tagtaaaacc ctgaataagt taactaaaga ctacaacgcc
    18241 gccagcgatc gcattgatgc gcaggtcgcg cgctacaaag aacaatttac ccaactggac
    18301 gttttaatga cctcgttaaa cagcaccagc agctacttaa cgcagcagtt cgaaaacaac
    18361 agtaattcca agtaatcatt attcatcggg agacaggtca tgtacgcggc aaaaggcacc
    18421 caggcctatg cacaaattgg cgtcgaaagc accgtaatga gcgccagcca acagcagttg
    18481 gtcaccatgc tatttgatgg agtgctgagc gcactggtta gagcgcgact gtttatgcag
    18541 gacaacaatc agcaaggcaa aggcgtctct ttgtcaaaag cgatcaacat cattgagaac
    18601 ggactgcggg tgagtcttga tgaagagagc aaagacgaat taacccaaaa cttaattgct
    18661 ctttatagct atatggtcag gcgtttgctg caagccaatt tacgcaacga tgtctcagca
    18721 gtcgaagagg tggaagcatt aatgcgtaat attgccgatg cctggaaaga gtctttactc
    18781 tccccttctt cgattcagga cccagtctga tgaacaatgc accgcatttg tatttcgcct
    18841 ggcaacaact cgtcgaaaaa agccagctca tgttacgcct ggcaacggaa gaacaatggg
    18901 acgaactcat cgctagcgaa atggcgtatg tgaatgcggt gcaggagatc gcacacctga
    18961 ctgaagaggt tgccccgtcc actacgatgc aggagcagct ccgcccgatg ctgcacctga
    19021 ttctcgacaa cgaaagcaag gtaaagcagt tattacagat tcggatggat gaactggcga
    19081 aactggtcgg tcagtcatcg gtgcaaaaat cggtgttaag tgcctatggc gatcagggcg
    19141 gctttgtgct ggctccgcag gataacctct tttgaatctt aatgagccga tggctcgcga
    19201 ataatccgat tacggctacg cttctaatat tccccttgaa tggagtcgaa gaatgcgtaa
    19261 tcccacgctg ttacaatgtt ttcactggta ttacccggaa ggcggtaagc tctggcctga
    19321 actggccgag cgcgcagacg gttttaatga tattggtatc aatatggtct ggttgccgcc
    19381 cgcctataaa ggcgcatcgg gcggctattc ggtcggctac gactcctatg atttatttga
    19441 tctaggtgag tttgatcaga aaggcagcat ccctactaaa tatggtgata aagcacaact
    19501 gctggccgcc attgacgccc tgaaacgtaa tgacattgcg gtgctgctgg acgtggtagt
    19561 caaccacaaa atgggcgcgg atgaaaaaga agctattcgc gtgcagcgtg taaatgctga
    19621 tgaccgtacg caaattgacg aagaaatcat tgagtgtgaa ggctggacgc gttacacctt
    19681 ccccgctcgt gccgggcaat actcgcagtt tatctgggat ttcaaatgtt ttagcggtat
    19741 cgaccatatc gaacaccctg atgaagatgg catttttaaa attgttaacg actataccgg
    19801 agaaggctgg aacgatcagg ttgatgatga actcggtaat ttcgattatc tgatgggtga
    19861 gaatatcgat tttcgcaatc atgccgtgac ggaagagatt aaatactggg cgcgctgggt
    19921 gatggaacaa acgcaatgcg acggctttcg tcttgatgcg gtcaaacata ttccggcatg
    19981 gttttataaa gagtggatcg aacacgtaca ggaagttgcg ccaaagccgc tgtttattgt
    20041 ggctgaatgc tggtcgcatg aagtcgataa actgcaaacg tatattgatc aggtggaagg
    20101 caaaaccatg ctgtttgatg cgccgctgca gatgaaattc catgaagcat cgcgcatggg
    20161 tcgcgactac gacatgacgc agatcttcac tgggacgctg gtggaagccg atccttttca
    20221 cgccgtaacc ctcgttgcca accacgacac ccaaccgttg caagccctcg aagcaccggt
    20281 cgaaccgtgg tttaaaccgc tggcgtatgc cttaattttg ttgcgggaaa atggcgttcc
    20341 ttcggtgttc tatccggacc tctacggtgc acattacgaa gatgtcggtg gtgacgggca
    20401 aacctatccg atagatatgc caataatcga acagcttgat gagttaattc tcgcccgtca
    20461 gcgtttcgcc cacggtgtac agacattatt tttcgaccat ccgaactgca ttgcctttag
    20521 ccgcagtggc accgacgaat atcccggctg cgtggtggtc atgtcgaacg gagatgatgg
    20581 cgaaaaaacc attcatctgg gagagaatta cggcaataaa acctggcgtg attttctggg
    20641 gaaccggcaa gagagcgtag tgacagacga aaacggcgaa gcgaccttct tttgcaacgg
    20701 cggcagcgtc agcgtgtggg ttatcgaaga ggtgatttaa atccatcccc ggcggcaagc
    20761 cggggagatt tcattacggc agtggcgtcg gtaacggcgc tttatccagc gcggcggcac
    20821 actctgccgt cgggcgatcc acacgctcca tcgtcatacc atcgtactca atggtgttac
    20881 cgtcacgctc aacctcgtac agctcacgtt ttacggtaac gttagtgaga tcatcagaca
    20941 tcaatgtcaa cttacccggc acggcaatcc cccgctgcca ctgacggcaa tccaaggtat
    21001 cgccctcttt agtgacaatc aggctgccta tcgcttccgg actgaccagc gcacgttgcg
    21061 gccctttggt ctgccagtag cccgccagcc agtccggcgc gggggttttc acgacattgt
    21121 tatagttttc gacttcggca cagcccgcca gcaacatgag cgcgcctgca attgcgagtt
    21181 ttttcatcat tcgtcctgca tgagaagaaa agaggattgt ggcattaaag ctctgatgtc
    21241 gccagccttt ccaggttcag agagaaattt gatctaacta ttatttttgt gaattattac
    21301 cccgaaaata aatttgcatg gtgtatgatt cgcggctctt gtttcaaacc ttctgagttc
    21361 agaggctaca ttcatgtcat ggcagcaatt caaacacgcc tggttgatta aattctgggc
    21421 acccatccct gcggtcatcg cggcgggtat tctctccact tactattttg gcattactgg
    21481 caccttttgg gctgtcacgg gtgaatttac ccgttggggc ggtcagctcc tgcagctgtt
    21541 tggcgtccat gctgaagagt ggggttattt taaaattatc catctggaag gatcgccatt
    21601 aacccggatc gacggcatga tgatcctcgg tatgtttggc ggctgctttg ctgctgcgct
    21661 gtgggccaac aatgtcaaac tacgcatgcc acgcagccgt atccgcatta tgcaggccat
    21721 cattggcggc attatcgccg gttttggcgc gcgtctggca atgggctgta acctggcggc
    21781 gttctttacc ggcattcctc agttctcgct gcatgcctgg ttctttgcca tcgccactgc
    21841 cattggttca tggtttggcg cgcgctttac cctgctgccc atcttccgta ttcccgtgaa
    21901 aatgcagaaa gtttctgccg cctcaccgct gacgcaaaaa ccggatcagg cgcggcgtcg
    21961 ttttcgtctc gggatgctgg tctttttcgg catgctgggc tgggcgctgc tcacggcgat
    22021 gaaccaacca aaactggggc tggcaatgct gtttggcgtc ggctttggtt tactgattga
    22081 acgtgcgcaa atctgcttta cttcggcgtt ccgcgatatg tggatcaccg gacgtaccca
    22141 tatggcgaaa gcaatcatta tcggtatggc ggtgagtgcc atcgggatct tcagttacgt
    22201 acagttaggc gttgaaccta aaatcatgtg ggcgggacca aatgcggtaa ttggtggttt
    22261 actttttggt tttggcatcg tgctggcagg cggctgcgaa accggctgga tgtaccgcgc
    22321 ggtagaaggc caggtgcact actggtgggt cggtctgggc aacgtgatcg gctcaacgat
    22381 tctggcgtat tactgggatg atttcgctcc ggcgctggcc accgactggg acaaaatcaa
    22441 cctgctgaaa acttttggtc cgatgggagg cctgctggtg acatatttgc tgctgtttgc
    22501 tgcgctgatg ttgattatcg gctgggaaaa acgcttcttc cgccgtgagg caccgcagac
    22561 tgctaaggag atcgcatgaa aaatatcgtt cctgattacc gccttgatat ggtgggtgaa
    22621 ccctgccctt atccggcagt cgcaaccctt gaggcgatgc cgcaattaaa aaaaggtgag
    22681 atcctggaag tggtgagcga ctgtccgcag tcgatcaata atattccact ggatgcgcgt
    22741 aatcacggct acacggtgct ggatattcag caagacgggc cgaccattcg ttatttaatt
    22801 cagaagtaat ccctcattcc cgctgtggcc cacagcggga acatcgttac ctctgacaca
    22861 tagattttac ccgccccctc tcctattctt ttcagtatga tctcaggagg ttgctatgtg
    22921 tggacgcttt gcccaatccc aaacgcgtga ggattacctt gcgcttctcg cggaagatat
    22981 tgaacgcgat attccctacg atcccgaacc cattggcaga tacaatgtcg cgccgggaac
    23041 caaagttctt ctgctcagtg aacgtgatga acaccttcat ctggatccgg ttttctgggg
    23101 atatgctccc ggatggtggg ataaaccgcc gctgattaac gcccgcgtag aaactgcctc
    23161 aaccagtcgc atgtttaaac cgctctggca acatggtcgg gcaatctgtt ttgccgatgg
    23221 ctggtttgag tggaaaaaag aaggcgacaa aaaaagcctt attttatcta tcgcgctgac
    23281 ggacaacctg tttttatggc cgcgataggt agcataccgt ttgagcgtgg tgacgaagcc
    23341 gaaggatttt tgatcgtcac tgcggcggca gatcaaggtc tggtagatat tcatgaccgc
    23401 cgcccgctgg ttctgtcgcc agaagctgca cgggaatgga tgcggcaaga cattggtggt
    23461 aaagaagcct cagaaatcgc taccagaagc tgtgtgccag caaaccagtt cacctggcac
    23521 cctgtgtcgc gcacggtggg taatgtcaaa aaccagggcg cggagttaat tcaacctgtt
    23581 tgttgacatc ctccacgccc tgaatgacga ggacccctgc tacgttcagg ctgtcgcctg
    23641 aatcatttcg gtgggttcct gcttcaacga gcggcctgac tgcaccatct ctccacaggc
    23701 aagaacggcg tgccccgccg ctaaaatgtt acgagctcca tttacatcgg cgttcgctgt
    23761 atatccacat acctggcatc tgaatttact ttgtgacagg cgattttctt tcgctgtatg
    23821 accacagcac gcgcaacgct ggcttgtgta cgctggcgga acagcaagca cctgaccgcc
    23881 acgccagagc tgcttgtact caagctggcg gcgcatttca taccagccct gatccagtat
    23941 cgaacggttt aaacctgatt ttgcccggac attgcgcccc ggctggctga ccgtacccgc
    24001 tgctgacttt gacatgttgc tgaccttcaa atcctcaatg actatcatta cgtggttttt
    24061 gctgacggtc gttgtgactt tgtgaaggta gtccctgcgg atatttgcga tacaggaatg
    24121 cagtcgctgt attttgcgtt tctgcttctg ccagttgttg ctgaatttga ccttgcggct
    24181 taactgtcgc tgaagtctcg ccagcgtctt ctggtttttc tgaaaactgt ttacaggctc
    24241 aaagactgtg ccatctgaca gcgaggcaag tttagccacg ccagcatcca gcccgaccat
    24301 tgatgctgaa gggtgaaccg gagttgatac ttcactttct gcctgaatac tgatgtacca
    24361 cttaccgcag gactggctga cagtgacatt tttcacaaca cccgtgacct gacggctgtt
    24421 ccggtagcgc atccagccaa gttttggcag aaaaatacgg ctgttttcct gagcgagctt
    24481 aacgccctgc ggatagcgga atgcatcatt ctgtcaccgc tttttgaatc aggggaacgc
    24541 cgagcgcttc tggaaaaagt ttttgcaggc ctgctcaggg tctttcagtg actgttgcaa
    24601 tggctgtgac ggagcatctt taatccattg cgtttcagtg gcatttttcc actcaaccaa
    24661 tcaggaagcc attttcgcgt aagggatgtc tttgttaccg gccttatgat tctcattctg
    24721 acgtgccaga gtacgactga aaacgaaacg acatgcgcct gcgaagcgcc tcatttcacg
    24781 cgcctgttga ccaccgggtc ttaaccggaa tttgaatacc tgtagtcgtt tcacagggca
    24841 aattatactt tggtctgtga aaaaaactga tatccgacgc ggaaggcact gtgttttcct
    24901 gatgcatgct caccgggtct ttgttaccaa gtaccgacgc cagatttttg accatgacgc
    24961 gacagaaaaa ctacgcactt acttttcaaa tgtatgtgct tattttgaag ctgaactggt
    25021 tgaaatggat ggcgaaccag atcacgtcca tctgttaatt aactatcccc ccaaactggc
    25081 gatatccagt ctggtaaaca aacagcctca aaggcgtatc gggtaggtta ctgcgacggg
    25141 atcgaccaga tattgctgcc aggtattacc gcaaagatgt tttgtggcgt cccggctatt
    25201 ttgccagtag ctgcggaggt gcgccaatat ccgtcatccg ccaatacatt gaacaacagc
    25261 aaacacctgg tcagatgaaa aaacgagcct tatatccccg ccctgaagga cgggctttta
    25321 cggtgcactg gataagcctg gagattatct acacctgcat ggacatcatt gtactatgcc
    25381 ccgtacatgc tccatcacat cctcaacatc aaagtattag agccttgcaa accattatgc
    25441 gtctcagttt gtctcatcta tcttacgcaa aaaaaataga actaatatca agcggcgcta
//
LOCUS       DAZPCS010000042        24716 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZPCS010000042 DAZPCS010000000
VERSION     DAZPCS010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24716
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.042"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(49..125)
                     /locus_tag="ACHUQ7_001738"
     tRNA            complement(49..125)
                     /locus_tag="ACHUQ7_001738"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(89..91),aa:Arg,seq:acg)
     gene            complement(129..221)
                     /locus_tag="ACHUQ7_001739"
     tRNA            complement(129..221)
                     /locus_tag="ACHUQ7_001739"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(185..187),aa:Ser,seq:gct)
     gene            complement(537..722)
                     /gene="csrA"
                     /locus_tag="ACHUQ7_001740"
     CDS             complement(537..722)
                     /gene="csrA"
                     /locus_tag="ACHUQ7_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFR1135178.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(957..3587)
                     /gene="alaS"
                     /locus_tag="ACHUQ7_001741"
     CDS             complement(957..3587)
                     /gene="alaS"
                     /locus_tag="ACHUQ7_001741"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFR1135179.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3715..4215)
                     /gene="recX"
                     /locus_tag="ACHUQ7_001742"
     CDS             complement(3715..4215)
                     /gene="recX"
                     /locus_tag="ACHUQ7_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFR1135180.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4458..5519)
                     /gene="recA"
                     /locus_tag="ACHUQ7_001743"
     CDS             complement(4458..5519)
                     /gene="recA"
                     /locus_tag="ACHUQ7_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFR1135181.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5599..6096)
                     /gene="pncC"
                     /locus_tag="ACHUQ7_001744"
     CDS             complement(5599..6096)
                     /gene="pncC"
                     /locus_tag="ACHUQ7_001744"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFR1135182.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6241..7326)
                     /gene="mltB"
                     /locus_tag="ACHUQ7_001745"
     CDS             complement(6241..7326)
                     /gene="mltB"
                     /locus_tag="ACHUQ7_001745"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFR1135183.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7582..8144
                     /gene="srlA"
                     /locus_tag="ACHUQ7_001746"
                     /gene_synonym="gutA"
                     /pseudo
     CDS             7582..8144
                     /gene="srlA"
                     /locus_tag="ACHUQ7_001746"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: protein motif:HMM:NF015565.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
     gene            8141..9100
                     /gene="srlE"
                     /locus_tag="ACHUQ7_001747"
                     /gene_synonym="gutE"
     CDS             8141..9100
                     /gene="srlE"
                     /locus_tag="ACHUQ7_001747"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFR1135184.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            9111..9482
                     /gene="srlB"
                     /locus_tag="ACHUQ7_001748"
                     /gene_synonym="gutB"
     CDS             9111..9482
                     /gene="srlB"
                     /locus_tag="ACHUQ7_001748"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFR1135185.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9486..10265
                     /gene="srlD"
                     /locus_tag="ACHUQ7_001749"
     CDS             9486..10265
                     /gene="srlD"
                     /locus_tag="ACHUQ7_001749"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HFR1135186.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSRMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10370..10729
                     /gene="gutM"
                     /locus_tag="ACHUQ7_001750"
     CDS             10370..10729
                     /gene="gutM"
                     /locus_tag="ACHUQ7_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFR1135187.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVDR
                     SSGRFKPRVVVAIALDDQQCIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10796..11569
                     /gene="srlR"
                     /locus_tag="ACHUQ7_001751"
                     /gene_synonym="gutR"
     CDS             10796..11569
                     /gene="srlR"
                     /locus_tag="ACHUQ7_001751"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFR1135188.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQIVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11562..12527
                     /gene="gutQ"
                     /locus_tag="ACHUQ7_001752"
     CDS             11562..12527
                     /gene="gutQ"
                     /locus_tag="ACHUQ7_001752"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFR1135189.1"
                     /translation="MSEALLNTGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12524..14038)
                     /gene="norR"
                     /locus_tag="ACHUQ7_001753"
     CDS             complement(12524..14038)
                     /gene="norR"
                     /locus_tag="ACHUQ7_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFR1135190.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14225..15664
                     /gene="norV"
                     /locus_tag="ACHUQ7_001754"
     CDS             14225..15664
                     /gene="norV"
                     /locus_tag="ACHUQ7_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFR1135191.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAMDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15661..16794
                     /gene="norW"
                     /locus_tag="ACHUQ7_001755"
     CDS             15661..16794
                     /gene="norW"
                     /locus_tag="ACHUQ7_001755"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFR1135192.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(16922..19174)
                     /gene="hypF"
                     /locus_tag="ACHUQ7_001756"
     CDS             complement(16922..19174)
                     /gene="hypF"
                     /locus_tag="ACHUQ7_001756"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFR1135193.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQALVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19327..19854)
                     /gene="hydN"
                     /locus_tag="ACHUQ7_001757"
     CDS             complement(19327..19854)
                     /gene="hydN"
                     /locus_tag="ACHUQ7_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFR1135194.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVPVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(20003..21013)
                     /gene="ascG"
                     /locus_tag="ACHUQ7_001758"
     CDS             complement(20003..21013)
                     /gene="ascG"
                     /locus_tag="ACHUQ7_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFR1135195.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESS
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            complement(21075..21146)
                     /locus_tag="ACHUQ7_001759"
                     /pseudo
     CDS             complement(21075..21146)
                     /locus_tag="ACHUQ7_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21156..22865)
                     /gene="hycE"
                     /locus_tag="ACHUQ7_001760"
     CDS             complement(21156..22865)
                     /gene="hycE"
                     /locus_tag="ACHUQ7_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFR1135196.1"
                     /translation="MSEEKLGQHYLAALNEAFLGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHPHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGLARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSYAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(22883..23806)
                     /gene="hycD"
                     /locus_tag="ACHUQ7_001761"
     CDS             complement(22883..23806)
                     /gene="hycD"
                     /locus_tag="ACHUQ7_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFR1135197.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(23809..>24716)
                     /locus_tag="ACHUQ7_001762"
     CDS             complement(23809..>24716)
                     /locus_tag="ACHUQ7_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HFR1135198.1"
                     /translation="TGIALEQPALIALGLVGGLYHLLNHSLFKSVLFLGAGSVWFRTG
                     HRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEWVIYQSFFKLSNSGAFVA
                     RLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAENATCAPLLMSVSVVALAI
                     CCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLIACPLLPFIIMAICKGDR
                     LPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKLRKWLNPVSLVPGWQCEG
                     SALLFRRMALVELAVLVVIIVSRGA"
BASE COUNT         5831 a         6950 c         6338 g         5597 t
ORIGIN      
        1 tactgcagat tttttgatac cgctactaaa gccgtatcaa gtaagagatg gtgcatccgg
       61 gaggattcga acctccgacc gctcggttcg tagccgagta ctctatccag ctgagctacg
      121 gatgcaaatg gcggtgaggc ggggattcga accccggatg cagcttttga ccgcatactc
      181 ccttagcagg ggagcgcctt cagcctctcg gccacctcac cacacgcctc ttacgagtgc
      241 ttcgaagaac ttgtttatcg ctcatcatcg ctgcgtggcg cacatattac tttctgagac
      301 ttataagtca aacaattttt cccacacttt tatcgtttgc acacttcacg ctcaattagt
      361 ctgtaaaagc gtcaaaaaga gtgttttatc tacagaaaaa cggaggtctg gcagatagca
      421 gtaatgcaaa aaaatggaga cttaagttga atgaacggga gtaaagcgaa aagactatag
      481 agtgaaggag aaattttgag ggtgcgtctc accgataaag atgagacgcg gaaagattag
      541 taactggact gctgggattt ttcagcctgg atacgctggt agatctcttc acggtgaaca
      601 gaaacttcct tcggggcatt tacgccaata cgtacctggt tgccctttac ccctaaaact
      661 gtcacggtga cctcatcccc aatcatgagg gtctcaccaa ctcgacgagt cagaatcagc
      721 attctttgct ccttgaaaga ttaaaagagt cgggtctctc tgtatcccgg cattatccat
      781 catataacgc caaaaagtaa gcaatgacaa acacattaca tgtaagcagt catggcatta
      841 cattctgtta aacctaagtt tagccgatat acacaacttc aacctgactt tatcgttgtc
      901 gatagcgtta atgcgaatgc cgtgaagcga gtccacggca ttgcctgacg cttatattat
      961 tgcaatttcg cgctgaccca gcctttcaca ctggctaacg ctgcaggtaa ggccgcagca
     1021 tccgtaccac cggcttgcgc catgtcagga cgtccaccac ccttgccgcc cacctgctga
     1081 gcgaccatac caatcagttc ccctgctttc acacgatctg tgacgtcctt agatacgcct
     1141 gcaatcagag aaaccttacc ttcggctacc gttgccagca cgataattgt cgaccccagc
     1201 tgatttttta aatcgtcaac catggtacgc aacattttcg gctcaacacc gctaagctcg
     1261 ctaaccaaca gcttaacacc attaacatca attgccttac tggaaagatt tgcgctctcc
     1321 tgtgcggcag cttgttcttt aagctgttgt agttcttttt ctaactgacg cgtacgttcc
     1381 agtactgagc gcactttatc cgccagatta ttgctatcgc ctttcagcag atgcgcgact
     1441 tcgcttaagc gatcgctgtc tgcatgaacg gtggtgatag cgccttctcc ggttaccgct
     1501 tcgatacgac gaacgcctgc agcagtaccc gattcagaga tgatgcggaa cagaccaata
     1561 tcaccagtgc ggctggcgtg agtaccgcca cacagctcgg tggagaaatc gcccatgctc
     1621 agcacgcgta cacgctcatc atacttctcg ccgaacagcg ccatcgcacc tttcgctttc
     1681 gccgcttcga gattcatgat gttggtttcg atcggcaaat tgcgacgaat ctgtgcgttc
     1741 accaggtctt cgaccgcacg aatctcttcc ggtttcatcg cttcgttgtg tgagaagtcg
     1801 aagcgcagca ccttgtcgtt aaccagtgaa cctttctgcg atacatgagt ccccagaacc
     1861 tggcgcagcg cagcgtgcat caggtgcgtt gcggagtgat tcagacgaat acgggcgcga
     1921 cgagcctcat caatatcagc ctgcacagcg tcgcccactt tcagagaacc cgcagcaagt
     1981 ttaccgatgt gaccaatcgc ctggccgtat ttctgcgtat cttccaccgc gaaggagaag
     2041 ttagcgcctt tcagttcgcc tttatcacca acctgaccgc cggattccgc atagaatggc
     2101 gtttgatcca gcacgaccac agcttcctgg ccggcattga tggcatcaac cgctttacca
     2161 tcaacaaaca gcgcggtcac tttgccgttc agttccagat ggtcatagcc tttaaattca
     2221 gatgcactat caacacggat cattgcgttg taatcggcac caaagccgct ggcttcgcgc
     2281 gcgcgacgac gctgctcttc cattgctgct tcaaaaccag cttcgtcaac tttgatgttg
     2341 cgctcacgac aaacatcagc cgtcaggtca accgggaagc cataggtgtc gtacagacgg
     2401 aaagccgttt caccatccag cgtatcacca gaaagttttg ccagctcttc atccagcaac
     2461 gccagaccgc gctccagagt acgggcaaac tgctcttctt cagtcttcag cacctgctca
     2521 acctgcgcct gctggcgttt caggtcttca cccgcagagc ccataacgtc gatcagcgga
     2581 ccaaccagtt tgtagaagaa ggtttctttc gcgccgagca tattgccgtg acgcactgcg
     2641 cgacgaatga tacgacgcag tacataacca cggttttcat tggacggcat tacgccatcc
     2701 gcgatcagga acgcacaaga acgaatgtgg tcagcgatta cgcgcagcga tttattgctc
     2761 agatcggtcg cgccagtgac tttcgctacc gcctggatca acgtgcggaa caggtcgatg
     2821 tcatagttag agttaacgtg ttgcagcacc gcagcaatac gctccagacc cataccggta
     2881 tctacagacg gcttcggcag cggttccatc gtgccatctg cctggcggtt gaactgcatg
     2941 aagacgatgt tccagatctc aatgtagcgg tcgccgtctt cttccggact tcccggaggg
     3001 ccaccccaaa tgtggtcgcc gtgatcgtag aagatttcgg tgcacgggcc gcacggacca
     3061 gtgtcaccca tctgccagaa gttgtcagat gcgtatggcg cacctttgtt atcgccgatg
     3121 cgaataatac gttcgcgcgg gatcccgact tctttttccc agatttcgta ggcttcgtcg
     3181 tcgctttcat agacggtaac ccacagacgc tctttcggca gggcaaacca tttttcgctg
     3241 gtcagcagtt cccatgcaaa ctgaatggca tcgtgtttga aatagtcgcc gaagctgaag
     3301 ttgcccagca tttcgaagaa ggtatggtga cgcgcggtgt aaccgacgtt ttccaggtcg
     3361 ttgtgtttac cacccgcacg cacgcagcgt tgggaagtgg tagcgcggga ataattacgc
     3421 ttgtcgagcc caaggaacac atccttgaac tggttcatcc cggcgttggt aaacaacaaa
     3481 gttgggtcgt tatggggtac cagggagctg ctggcaacta cctgatgtcc cttactatgg
     3541 aaaaagtcga gaaacgcctg acggatctca gcggtgctct tgctcataat tatcctgaaa
     3601 tcaagctaac gaaatatcgc caccagctcc agcgtgtctt aaccgccggg ctggtaactg
     3661 aaaagtggga ataagataag ttttcttgac tgggaagtaa aataccgtat gcgttcagtc
     3721 ggcaaaattt cgccaaatat cctggatatc ttccatcaga tagccacgat agagcagaaa
     3781 acgctggatc ttaacttttt ctgaaaagac agttggcaaa ggttcaccat attttcgcgt
     3841 cgcctgatcg cgcgccagtg cgcaccagtc gatgtcacat tcacgcatcg ctttttctgt
     3901 cgcttcgcgg gaaatacctt tctgattcag ttcctgacga atacgtgcag gtccataccc
     3961 tttgcggcta cggctggcga taaagcgcgc aacaaatcgg ctgtcatcga gatagccatg
     4021 ttcatggcac caggcaataa cgcgctcgta atcttctgcc gtagcatcaa tctcttctgg
     4081 gccatttttg cccataatcg gtgccgcgag tttacgtcgc agttcttgct cactgtgatc
     4141 gcgcaccgcc agaatgcgta ccgcacgatc caacaggcga gcatatgccg ggcgacggga
     4201 tgttgattct gtcatggcat atccttacaa cttaaaaaag caaaagggcc gcagatgcgc
     4261 cccaggtttt gctgataaac agatttaata gtcaatgagt atgcagaatg ttcccgcagg
     4321 cccaattcgt tagaggatcg aggtgagagt aatttttcac gccagtgctg gcctgttccc
     4381 ctcaccctaa ccctctcccc aaaggggcga ggggaccgtc cgtgcacaca ttatctatta
     4441 tcatcaataa cgaacgatta aaaatcttcg ttagtttctg ctactccttc gctgtcatct
     4501 acagagaaat ccggcgttga gttcgggttg ctcagcagca actcacgtac tttcttctcg
     4561 atctctttcg cggtttccgg gttatctttc agccaggcag ttgcattcgc tttaccctga
     4621 ccgatcttct cacctttgta gctgtaccac gcgcctgctt tctcgatcag cttctctttt
     4681 acgcccaggt caaccagttc gccgtagaag ttgatacctt cgccgtagag gatctgaaat
     4741 tcagcctgtt taaacggtgc agcgattttg ttcttcacca ctttcacgcg ggtttcgcta
     4801 cccaccacgt tttcgccctc tttcaccgcg ccgatacgac ggatgtcgag acgaacagag
     4861 gcgtagaatt tcagcgcgtt accaccggta gtggtttccg ggttaccgaa catcacacca
     4921 attttcatac ggatctggtt gatgaagatc agcagcgtgt tggactgctt caggttaccc
     4981 gccagcttac gcatcgcctg gctcatcata cgtgccgcaa ggcccatgtg agagtcgccg
     5041 atttcgcctt cgatttccgc tttcggcgtc agtgccgcca cggagtcaac gacgataacg
     5101 tctactgcgc cagaacgcgc cagggcgtca cagatttcca gtgcctgctc gccggtatcc
     5161 ggctgggagc acagcaggtt atcgatatcg acgcccagtt tacgtgcgta gattgggtcc
     5221 agcgcgtgtt cagcatcgat aaacgcacag gttttacctt cacgctgcgc tgcggcgatc
     5281 acctgcagcg tcagcgtggt tttaccggaa gattccggtc cgtagatttc gacgatacgg
     5341 cccatcggca gaccacctgc cccaagcgcg atatccagtg aaagcgaacc ggtagagatg
     5401 gtttccacat ccatggaacg gtcttcaccc aggcgcatga tggagccttt accaaattgt
     5461 ttctcaatct ggcccagtgc tgccgccaac gctttctgtt tgttttcgtc gatagccatt
     5521 tttactcctg tcatgccggg taataccgga tagtcaatat gttctgttga aacaattata
     5581 ctgtatgctc atacagtatc aagtgttttg tagaaattgt tgccacaagg tctgcaatgc
     5641 atacgcagta gcctgacgac gcaccgcatc acggtcgccg ctgaagcatt cccgccgggt
     5701 aatgccttca ccgcgggcag tggcaaaagc aaaccagacg gtgccgacag gcttctcttc
     5761 actgccgcca tccggcccgg cgataccact aatagacacg gcataatcag cacgagccgc
     5821 tttcagtgca cctatcgcca tttccaccac gacgggttca ctcaccgcgc catgctgcgc
     5881 cagcgtctct tcgcgtacgc cgatcatctg cgctttggct tcgttactgt aggtgacaaa
     5941 tccgcgttca aaccaggcgg agctaccggc aatatcggta atcactttcg ctacccaacc
     6001 accggtacaa gactcggcag ttgttacggt tgcgccacgg gctttcagcg cctgcccaac
     6061 ctgttcgctt aactgcatca gttcactgtc agtcatcaca acctctgtca gtcaaaaatt
     6121 tatgccggac aagatagcac tttcgtcagg aagatggtgt tggcgttgca attttacgag
     6181 gaggattcag aaaaaagctg attagccaga gggaagctca cgcccccctc ttgtaaatag
     6241 ctactgtact cgcgccagcg ccacggcttg tcctaactgc cagaccgcca ttgcgtaatg
     6301 ggtgctgtgg ttgtaacggg tgatggtgta gaagttcggc agaccgtacc agtactggta
     6361 gccggtgcca acatccagac gcagcaggct ggcttgttga tggttgccca gcggctgctg
     6421 tggcgttaaa cctgcggcgg caagctgcga aatgctgtac ttagttttga aaccatttgg
     6481 caagcctgga gcctgaccgt ttgccattat cgcgacctga tcgcctttca cccagccgtg
     6541 cgctttgaaa tagttcgcca cgctaccgat cgcatcaacc ggatcccaca ggttgatatg
     6601 cccgtcgccg ctgaaatcta ccgcatattg tttgtaagac gacggcataa actgtccgta
     6661 gcccatcgcc ccggcaaagg aacctttcag attgagcgga tcgtcctgct cgtcgcgcgc
     6721 catcagcagg aaggtttcca gctcgccaga gaaatactcc gcgcggcgtg ggtagttaaa
     6781 tgacagcgtt gccagcgcat cgaggatgcg agttttcccc atcacgcgcc cccagcgggt
     6841 ttcaacgccg ataatcccga cgataatttc cggcggtacg ccatacacct gccacgcgcg
     6901 attcaacgca tcttcatact gattccagaa aaccacaccg ttctgcacgt tgtccggcgt
     6961 aataaatttt ttgcgataac gcagccatgc gccgttcgga cctgatggcg gtttcaccga
     7021 tgtggttggt gcctggttat ccatcagccg cagtaccgaa tccagacgct tcgcctggga
     7081 gagaatttcc tgcaactgct gacgatcgaa accgtgtttg ttcaccattt tgtcgatgaa
     7141 ctgctgggca ttcgggttat tagcgaaatc gccgcccatc tgcatcacat tgtgctgcgg
     7201 ctcaagcagg aagccgccag acggcgttcc ggtggtcgtt tcagtctcag taggttttgg
     7261 cttgctgcta caggcggcaa gcaacacaaa aaggggaagc aatgttacat aacgacgctt
     7321 gaacatgagg ggtccattta acagattcaa ccaggggcaa gtatggtaaa gcatcacacc
     7381 ccgcacaagg aagcggtagt cactgcccga tacggacttt acataactca actcattccc
     7441 ctcgctatac ttttattcaa actttcaaat taaaatattt atctttcatt ttacgatcaa
     7501 aataacactt ttaaaccttt caacctgatt agattaggtt gccgtttggt aataaaacaa
     7561 taaatcctga aggagagaac aatgatagaa accattactc atggtgcaga gtggtttatc
     7621 gggctgttcc aaaagggcgg agaggtgttt accgggatgg tgaccggcat tcttccgctg
     7681 ttgattagcc tgcaggttat catgaacgca ctgattaatt ttatcggtca gcatcgtatt
     7741 gaacgttttg ctcaacgttg cgccggtaac cctgtttccc gttacctact gttaccgtgc
     7801 attggcacgt ttgtcttttg caatccgatg accttaagcc tgggtcgctt tatgccggaa
     7861 aagtacaaac ccagctacta cgcggcggcc tcttatagct gccactcaat gaatggcctc
     7921 ttcccccata tcaaccctgc gaactgtttg tttatcttgg cattgccagc ggtctgacaa
     7981 cgctgaacct gccacttggc ccactggcgg tgagttatct gctggttggt ctggtcacca
     8041 atttcttccg cggctgggtg accgatctga ccaccgccat ttttgagaaa aagatgggca
     8101 ttcaacttga acaaaaagtt cacctggcag gagcaacatc atgacgcgta ttcggatcga
     8161 aaaaggaacg ggtggctggg gcggcccgct tgagctggaa gccacgccgg gcaaaaaaat
     8221 cgtctatatc accgccggta cccggcctgc gattgttgac aaactggcac aacttactgg
     8281 ctggcaggct attgacggat ttaaagaagg tgaacccgcg gaggcggaaa ttggtgtcgc
     8341 ggtaatcgac tgtggcggca cattacgctg cggcatctat ccgaaacgac gtattcccac
     8401 cattaatatc cactcgacgg gcaagtccgg tccgctggcg cagtacattg tggaagatat
     8461 ttatgtctct ggcgtaaaag aagaaaacat cactgtagtg ggtgatgcga caccacaacc
     8521 ctcttccgtg ggccgtgact atgacaccag taagaaaatc accgaacaaa gcgatggttt
     8581 actggcgaag gtgggaatgg gcatggggtc cgccgttgcc gtgttgtttc agtctggtcg
     8641 tgacaccatc gacactgtat taaaaaccat tctgccgttt atggcattcg tctcggcgct
     8701 cattggcatc attatggctt ctggccttgg tgactggatt gcccacggtc ttgctccgct
     8761 agcgagccat ccactgggtc tggtcatgct ggcgctcatc tgctccttcc cactgctttc
     8821 acctttcctc ggcccaggcg cagttatcgc acaggttatc ggcgtattga ttggcgtgca
     8881 gattggtctc ggcaatattc cgccgcatct ggctttaccg gcactgtttg ccatcaacgc
     8941 gcaggcggct tgcgacttca tcccggtcgg tttgtcgctg gcggaagccc gtcaggacac
     9001 ggttcgcgtc ggtgtccctt ctgtactggt gagccgcttt ttaaccggcg cgccgactgt
     9061 actgatcgcc tggtttgtct ccggttttat ctatcaatag aggctgaaac atgaccgtta
     9121 tttatcagac caccatcacc cgtatcggcg cgagtgccac tgacgccctc agcgaccaga
     9181 tgctcatcac ctttcgtgaa ggcgcgccag cggacctcga agagtattgc ttcattcatt
     9241 gccacggcga gttgaaaggt gcactccatc ccggtttgca attttcactc gggcagcatc
     9301 gctatccggt gaccgctgtt ggcagcgtgg cggaagacaa ccttcgcgaa ctgggtcatg
     9361 tcaccctgcg cttcgatggt ttaaacgaag cggaatttcc gggcactgtc catgtggcag
     9421 gccctgtacc cgacgatatc gcgccgggat cggttttgaa gtttgaatct gttaaggagt
     9481 aaaaaatgaa tcaggttgcc gttgtcatcg gtggtgggca aaccttaggc gcgttcctgt
     9541 gccacggtct ggctgccgag gggtatcgcg tcgcggttgt cgatattcag agcgacaaag
     9601 ccgcaaatgt ggcacaagaa attaacgccg aatatggtga aggtatggcg tacggttttg
     9661 gtgctgacgc cactagcgag caaagcgttc tggcgctctc tcgtggggta gatgaaatct
     9721 ttggtcgcgt ggatttgctg gtctatagcg ccggaatagc aaaagcagcc tttatcagcg
     9781 acttccagct cggcgatttt gaccgttcgc tacaagtgaa tctggtgggt tatttcctgt
     9841 gtgcccgtga attttcgcgt ttgatgatcc gcgacgggat tcagggtcgc attattcaga
     9901 tcaactcgaa atccggcaaa gtgggcagca aacacaactc tggctacagc gcagcgaaat
     9961 ttggtggcgt cgggctgact caatcactgg cgctggatct ggcggagtac ggcattacgg
    10021 tgcattcact gatgctcggt aacctgctga aatcgcggat gttccagtca ctgttgccac
    10081 aatacgcgac caagctgggt atcaaaccgg atcaagtcga gcagtattac atcgacaaag
    10141 taccgctcaa acgcggctgc gattatcaag atgtgctgaa tatgctgctg ttctacgcca
    10201 gtcctaaggc gtcgtactgc accggacagt cgatcaatgt caccggcggt caggtgatgt
    10261 tctgatcaac agcggagatc cgttaaggat ctccgtgaga ctatagaatg cctgatgcgc
    10321 tacgctcatc aggcatacag gacttccgcc actacattaa ggaaaagtta tggtatccgc
    10381 actcatcacc gtcgccgtta tcgcctggtg tgcgcaactg gccttaggcg gctggcaaat
    10441 ttctcgtttt aaccgtgcct tcgacacact atgccagcaa gggcgggttg gcgtggaccg
    10501 ttccagcggg cgctttaaac cgcgggtcgt ggtcgccatc gcgctggacg atcagcagtg
    10561 catcgtcgac accttgttta tgaaaggact gactgtcttc gcccgaccgc aaaaaattcc
    10621 cgcaattacc ggcatgcatg cgggtgattt acagcccgat gtgatctttc cccatgatcc
    10681 actatcacag aatgctctat cattggcgct taaactgaaa cgtggataat ttcgttgtga
    10741 atgttacttg cttgcgaagt tatcattttg aaacctaaat caggtaatca cgcccatgaa
    10801 acctcgtcag cgtcaggccg ccattctgga gtatctgcaa aagcagggta aatgctcggt
    10861 tgaagaattg gcgcaatact ttgacaccac aggcacaacc attcgcaaag atctggtcat
    10921 tctggaacat gccggaaccg tcattcgtac ttatggcgga gtggtgttga ataaagagga
    10981 atccgatccg cctatcgatc ataaaacgct catcaacacc cacaagaaag agctgattgc
    11041 agaagctgcc gttagtttta tccatgatgg cgattcgatc attcttgatg ctggcagtac
    11101 cgttttgcag attgttcccc tgctctcgcg ctttaataac atcacggtga tgaccaacag
    11161 cctgcatatc gtcaatgcgc tatccgaact ggataacgaa caaactatcc tgatgccagg
    11221 cggaacattt cgcaaaaaat cggcctcatt tcacgggcaa ctggcagaga atgccttcga
    11281 gcatttcacc ttcgataaat tgtttatggg caccgacggc atcgatctca atgcgggcgt
    11341 aaccacgttc aacgaggttt ataccgtcag taaggcaatg tgcaatgccg cgcgcgaagt
    11401 gattttgatg gcggactcat caaagtttgg ccgtaaaagc cccaacgtag tttgcagtct
    11461 tgaaagcgtc gataagctaa ttaccgacgc aggtatcgat ccggcgtttc gtcaggcgct
    11521 ggaagagaaa gggatcgatg tgatcataac cggagagagc aatgagtgaa gcactactga
    11581 acacgggacg tcagacgtta atgctggagt tgcaggaagc aagccgttta ccggaacgtc
    11641 tgggcgatga ttttgttcgc gccgccaata tcatcctgca ctgcgaaggc aaagtggtgg
    11701 tttcgggaat tggcaaatcg ggccacattg gtaagaaaat cgccgcaaca cttgccagta
    11761 ccggcacacc ggcttttttt gtccatccgg cagaagcact gcacggcgat ctggggatga
    11821 ttgaaagccg cgatgtgatg ctgtttatct cttactccgg tggcgcgaag gaactggatc
    11881 tgattattcc gcgtctggaa gataaatcta tcgcgctgct ggcgatgacc ggcaaaccga
    11941 cgtcaccgct gggcctggcg gcgaaagcgg tgctggatat ctccgtagaa cgcgaagcct
    12001 gcccgatgca ccttgcgccg acctccagca ccgtcaatac cctgatgatg ggtgacgcgc
    12061 tggcgatggc ggtcatgcag gcgcgcggtt ttaatgaaga agattttgcc cgctcccacc
    12121 cagctggggc actgggcgct cgcttgctga ataaagtgca tcatctgatg cgtcgtgatg
    12181 acgctatccc acaggtagcg ttaaccgcca gcgtgatgga tgcgatgctg gaactcagcc
    12241 gcaccggtct ggggctggtg gcggtatgtg acgctcaaca acaggtacaa ggcgtcttta
    12301 ccgacggcga tttacgtcgc tggctggtag gcggcggcgc actcaccacg ccagttaatg
    12361 aagcgatgac gacaggcggc accacattac aggcacaaag tcgcgccatc gacgccaaag
    12421 aagttctgat gaaacgcaaa atcactgccg caccagtggt agatgaaaac ggcaaactca
    12481 ccggcgcaat taacctgcag gatttctatc aggccgggat tatttaatcc ttcaatccca
    12541 gacgtttcgc cagccgatgc aggttggcga cgtcagtttc cagcattcgc gcacaggctg
    12601 cccagttgtg atgattttgt gccagtgcct ggcgaatagt ttcacgctgg aacgcttctg
    12661 tcgcttcacg caggttttgc ttaacaacgg gcaccgccgc cgcttctggc ggcggcaacg
    12721 tcacctcagg aaaagcaaaa tgttgcgcct caagaatcac ttcatcgccg ttgcgggtgg
    12781 ctctcgccag cactaccgcc cgatgaatag catgttccag ttcgcgcacg tttcccggaa
    12841 aactgtagtg ttgcagtaaa tttcgcgccc cggcacttaa taccacgcgg gagagcccct
    12901 gccgcaaacg acactgctcg cagaaatacc ccgccagcag aatgacatca tcgccccgct
    12961 cacgcagcgg cggcaccgaa agtggaaaca cgctcaggcg atgaaacaaa tcggcgcgga
    13021 atcgccctgc cagcacctct tcgcgtaagt cgcggttagt cgccgccagc acgcgcacat
    13081 cgacccgcaa actacggtca tcgccaacgc gctgaatatc gccatactgc aacaccctca
    13141 gcagcttggc ctgcaatgcc aacgacaact cgccgatctc atcgagaaac agagtaccgt
    13201 tatccgccat ttcaaacttc ccgctgcggt tactgatagc gccagtaaac gctcctttca
    13261 catgcccgaa caactcactt tccgccacac tttccggcag tgcggcacag ttgagataga
    13321 ccagcggatt caccgcccgt ggcgaggctt catgaatcgc tttcgccacc agctccttac
    13381 cggttcccgt ctcaccgctg atcagaacgt tgagatcgga cgccgccaca atctcaatct
    13441 cttttttcag ttgcgtcatg ccaggggaca agccaatcat ctgcgtctgt ttcaccgctt
    13501 caaacggcgt ggcatcgcct ggcagcatat tctggctttc cagttgttca atcagcaacg
    13561 cattgcttaa cgctcccgcc gccagcgcgg caatcagccg taactcttcg tcgctgaaaa
    13621 catcgaactg atcgggctgc atcccgtcga gcgtcagtgc gccgatcagg ttttgtccga
    13681 caaacaatgg cagaccaacg caggcgtgaa ccttcagact ctcctgccca ggaatcaaac
    13741 cgtcataggg atcgggcaat tcgctgtctg cgggaaagcg caccacatcc ccggcgcggg
    13801 caatcgcttc cagccgtgga tgcccttcca gcgcaaagcg tctaccgagt acatcctttg
    13861 ccagaccgtc gatggcaagc ggaataaact gccgcgaatc gtaacgtagc aacgcagacg
    13921 catcgcactc cagcacctga cgtagcgtgg tgatcaggcg ctgaaaacga tcctgatgac
    13981 caatcccacg ctgcaattcg atggcgatat tcgccagcac atcaacggaa aaactcatct
    14041 ttgcctcact gtcaatttga ctatagatat tgtcatatcg accatttgat tgatagtcat
    14101 tttgactact cattaatgag cataatttta tttatagagt aaaaacaatc agataagaaa
    14161 ctggcacgca atctgcaatt agcaagacat ctttttagaa cacgctgaat aaattgaggt
    14221 tgctatgtct attgtggtga aaaataacat tcattgggtg ggtcaacgtg actgggaagt
    14281 gcgtgatttt cacggcacgg aatataaaac gctgcgcggc agcagctaca acagctacct
    14341 catccgcgaa gaaaaaaacg tgctgatcga caccgtcgac cataaattca gccgcgaatt
    14401 tgtgcagaac ctgcgtaatg aaatcgatct ggcggatatc gattacatcg tgattaacca
    14461 tgcagaagag gaccacgccg gggcgctgac cgaactgatg gcgcaaattc ccgatacgcc
    14521 gatctactgt actgccaacg ctatcgactc gataaatggt catcaccatc atccggagtg
    14581 gaattttaat gtggtgaaaa ctggcgacac gctggatatc ggcaacggca aacagctcat
    14641 ttttgtcgaa acaccaatgc tgcactggcc ggacagcatg atgacttacc tgacaggcga
    14701 cgcggtgctg ttcagtaacg atgctttcgg tcagcactac tgcgacgagc atctgttcaa
    14761 cgatgaagtg gatcagacgg agcttttcga gcagtgccag cgttactacg ccaatatcct
    14821 gacgccgttc agccgcctgg taacgccgaa aattaccgag atcctgggct ttaacttacc
    14881 agtcgatatg atagcgacct cccacggcgt ggtctggcgc gataacccga cgcaaattgt
    14941 cgagctgtac ctgaagtggg ccgctgatta tcaggaagac agaatcacca ttttctacga
    15001 caccatgtcg aataacaccc gcatgatggc tgacgctatc gcccagggaa ttgcggaaac
    15061 cgacccacgc gtggcggtga aaattttcaa cgtcgcccga agcgataaaa acgaaatcct
    15121 gaccaatgtc ttccgctcaa aaggcgtgct ggtcggcact tcgacgatga ataacgtgat
    15181 gatgccgaaa atcgccgggc tggtggagga gatgaccgga ttacgcttcc gtaacaaacg
    15241 cgccagtgct ttcggatctc acggctggag cggcggtgcg atggatcgcc tttccacgcg
    15301 cctgcaggat gcgggtttcg aaatgtcgct cagcctgaaa gccaaatggc gaccagacca
    15361 ggacgctctg gagttatgcc gtgaacacgg tcgcgaaatc gcccgtcagt gggcgctcgc
    15421 gccgctgccg cagagcacgg tgaatacggt agttaaagaa gaaacctctg ccacgacgac
    15481 ggctgacctc ggcccacgaa tgcagtgcag cgtctgccag tggatttacg atccggcaaa
    15541 aggcgagcca atgcaggacg ttgcgccagg aacgccgtgg agtgaagtcc cggataactt
    15601 cctctgcccg gaatgctccc tcggcaaaga cgtctttgac gaactagcat cggaggcaaa
    15661 atgagcaacg gcattgtgat catcggttcg ggcttcgccg cccgccaact ggtgaaaaat
    15721 attcgcaaac aggacgccac tattccatta accctgattg ccgccgacag catagatgag
    15781 tacaacaaac ccgacctcag ccatgttatc agtcaggggc aacgtgccga cgaccttacc
    15841 cgccagacgg cgggtgaatt tgccgggcag tttaatctgc gcctgtttcc gcacacctgg
    15901 gtgacggata tcgatgccga agcccatgtg gtgaaaagcc agaataatca gtggcaatac
    15961 gacaagttag tgctggcaac cggtgccagc gcctttgtcc cgcctgtgcc tgggcgtgag
    16021 ttaatgctga cgttaaatag tcagcaagag tatcgcgcct gtgaaacgca actgcgggat
    16081 gcccgacgcg tgttgattgt cggcggtggt ttgattggta gcgaactggc gatggatttt
    16141 tgtcgtgcag gcaaagcggt cacgctaatc gacaacgctg ccagtattct ggcgtcatta
    16201 atgccaccag aagtaagcag ccgcttgcag catcggttga cggagatggg cgttcatctg
    16261 ctgttgaaat ctcagttgca ggggctggaa aaaacggatt ctggcattct ggcaacgctg
    16321 gaccaccagc gtagtatcga agtggatgcg gtaattgccg ccaccggact gcgcccggaa
    16381 acagccctgg cacgacgcgc cgggctgacg attaatcgcg gcgtttgcgt cgatagttat
    16441 ctgcaaacca gtaatgccga tatttacgcg ctgggcgatt gcgcggaaat taacggtcag
    16501 gtattgccgt tcctgcagcc gattcaactt agcgggatgg tgctggcaaa aaatcttctc
    16561 ggcaataaca cgccgctgaa actaccggcg atgctggtga aaatcaaaac gccggaatta
    16621 ccgctgcatc tggcaggcga aacccagcgt caggatttac gctggcagat taataccgaa
    16681 cgccagggaa tggtggcgcg cggcgttgac gatgctgacc agcttcgcgc ctttgtggtc
    16741 agtgaggatc ggatgaaaga ggcatttgta ttgttgaaaa cgttgccggt gtaggtgggc
    16801 tactgtgcct aaaatgtcgg atgcgacgct ggcgcgtctt atccgaccta cggggacgca
    16861 tgtgtaggcc ggataaggca tttacgccgc atccggcaag ggtgtccaaa tgcaacacgc
    16921 tttatccgtt ctggacttca cccgctaacc aacgcgccgc agcaataacc ccctgcccca
    16981 gagacaaacc gccatcaccc gccggtaaac tctgtggaaa gagcaatgtg aaatcagcga
    17041 gataatgcgc cagacgtgca cgcagcaaac ggttatgaat aaccccgccg ctaaatacca
    17101 gcgtagtgat accacgcatc gtggcctgct cacgcatcaa cgcggcaaaa ccctgcgcca
    17161 gcgcatcatg aaacgcccac gcgcgttgat taaccagtgc ctgccagttc agccactgct
    17221 gccagaaagt tgcgagatcc agttgattgt ccacccgcgg catcgtcacc ggatgcgtca
    17281 ctccgtcgca tgaggctgcg agcgcctcca gagcacaagc cgcttcacct tcataactta
    17341 acgtggctgg cgcacagccc agtgccgccg ccactgcatc gaacaaccgc ccacacgatg
    17401 atgccagcgg cgcgttaatt ccacgctcaa tggcccgcgc cagtacgctc cagttttgct
    17461 gttgcacact tgccgtttca gggtaattct gccactccgg cacaaagcgc aggcactgcg
    17521 ccagcaggtt tcgccacggc tgcttcgccg ccaaatcgcc acccggaagc gccactgcag
    17581 gcaagccgcc caggtgctca cattcgcgat agttcacccg caggcactcg ccgccccaca
    17641 aagcgccatt ctcccccata ccgataccgt cgagcgtcaa agcaatgaca tcaccgccat
    17701 ccagcggcca ctgatgctct gccagacacg ccgctgcatg ggcatgatga tgcagtaccg
    17761 tttgcgtcgg cagattcatt tcgctcgccc actggctgga gacatagccc ggatgcacat
    17821 catgcacaat gtattgcgga gtgaaatcgt agatgttttg catcaggcgt aacgcttcgc
    17881 gccactgcat ctggatgcca tcgtcactta aatcgcccag atgctgactc aacaccgctt
    17941 gttcaccgcg caccaggcag aaggtatttt tcagatccgc gccaagacac agcacaggcg
    18001 gaacattttt aaagcccgga ggcaaaacca gcgcatccgg cacatacccc cgcgaacggc
    18061 gcagcatttc gccgctttcg cgcaccactg aatcatccat ccgctgcacg atgtcgcggt
    18121 tatgtagcaa gaatccgtcg gcaatgccct gcaaatccgc cagcgcctgt tcgttgctga
    18181 tagctggtgg tttaccgctc aggttgccgg aggtcatcac cagcgggcat tgcagttcct
    18241 gtaacagcaa atgctggagc gggttcgcag gcaacatcac cccgacttcg ttaaggccag
    18301 gggcgatatc atcacaaagc tcaggtacgt attttttatc caccagcaca atcggcgcag
    18361 cgggcgtggt aagcaactgg cgcgcagcgt ctggtaaact ttctgccact ggcaacatga
    18421 ctgccagcgg tttcgccggg cgatgtttgc gctcccgcag tgtcgccacc gcgttactgt
    18481 tacgtgcatc gcaggcaaga tgaaatccgc caatcccttt gatggcgaca atgttgccca
    18541 tttttaactg tgcgatagct gcctgtaatg ccgcctcttg ttctgcatgt tcaccatgac
    18601 ttacccattc aagatgcggg ccacactccg ggcaggccac cggctgggcg tggaagcgac
    18661 gatcgagcgg gtcgcggtac tctttgtcac aggccggaca tagcggaaac gccgccatca
    18721 cggtaaacgg gcggtcgtaa ggcatcgcgc gaataatggt gaaacgcggg ccgcagtggg
    18781 tacagttgat aaacggataa cgataacgcc gctcgcctgg ggtattcatt tcggcaaggc
    18841 aagcagggca agtggcggca tccgggacaa tttgcgtatt catggtgccg ccggtgctct
    18901 ggcggatggt gaactcggtg ggcagttgtg accagataaa cggctcacgc tcgacgctat
    18961 caatacgcgc cagtggcgga cagtgctgat gcaattgaac aagaaacgtt tccgggtctt
    19021 ccagcagccg gacttctacg ccatcgccgt cattacatac atcgccgtga agatttaatt
    19081 gctgtgccag ctgccagaca aacggacgaa aaccgacgcc ctgcactttg ccacgaatac
    19141 gcagttggac accgcaagat gtgttttttg ccattgagtt attcccgcca tcatgaattg
    19201 cgtaacccgc cctgccggac acgacagcgt cgtatccggc agtctcaggt cggcgatacc
    19261 gcccgctccg tattctacga atatttccgg gaattccttt gatgccagaa cagttctgta
    19321 agatttttag aacatcagcg ccgtacggcg gcgtttttct gcgctcagtt gttcaagttt
    19381 attacgatcg acacaaatca gcgcatgggt cgggcaagcc gccatacacg ccgggccgtc
    19441 ttcacgatgg ttgcacaggt cgcatttatt ggcttcggct ttgtcagccc gtacattcag
    19501 acccgcgccg ctgttgcgaa tcacaggacg taccaccact tccatcgcgc catacgggca
    19561 agccaccacg caggttttgc aaccaatgca acgttcctgc atcacaggaa caaacccttt
    19621 atcacggctg atagcaccat tcgggcagac gttagcgcac ggtgcatctt cacactgacg
    19681 gcaaactgtc gccgtggaaa tgttcacacc tttaatgaca tggatacgcg gtaaaaatgt
    19741 ttccggggtc agcgatgcac agtcctgatt ttcctgatga gaaaccacgc acgccacttc
    19801 acaggtacgg caaccaatac atttactcgc gtcagcaatg atgaaacggt tcatcaaatt
    19861 ctccagcaat gacagttaat gcgccgatac actcacaaat catgccagtt tttaatttgc
    19921 tgttatttaa ggaaattaat ttctgtaatg caggaaaaaa gatgtcatcg acactagtga
    19981 cgatgacatg tgatgacaat gtttatcgcg aaagggcaat gagtgagtcg cggcggatca
    20041 gttttccgct aaaggttttc ggcggtgaga aatccccgcc atcgagcata aaaatcagcc
    20101 gtccaataat ttcctgaatc atctcagtca ccggaatttt tacgctggag agcgccggaa
    20161 cgatataggg ggcaattgcg atatcatcga atccgataac tgacacctgc tctggcaccg
    20221 ctacgccgcg ctcgtgcaac gctttgatcg cacctatcgc catatcgtcg ttactggcaa
    20281 ctaacgcgct aaatttagcc cctcgttcga gcaacgtttc taccccttcg gccccgctgg
    20341 cgggcgtcca tttaccgtta gcgataagtt tttcattgag cgcaatacca tgctgcgaca
    20401 gcgcgtcttt atacccggca agacgttcaa tgctggtggg ggaatccatc gagccggtaa
    20461 ggaaagcaat ctcctgatgc ccggcgttta tcaactctgc cacggcgtta aagctggtct
    20521 gtttatgatc gcaccagacg ctatggctgc tattattgcg caggcggcga ttaagcacca
    20581 ttatcggctg actgtgcgcg tcaatgatgt catcaatctc atccacgctt aaaaaacgcg
    20641 ggtaaatcat aatggcgtcg cagcgcaaat ccagcagata ctgaatcgcc tggcgctctt
    20701 cttcggcgct gtgtttacca tctgccaata gcaactgtcg ccctttctct tccgccattc
    20761 gcgcggcatg aaacagtaat tcactaaaat aaatgccgtg ataaagcgtg ttggtcacta
    20821 ccagccccag cgtctgagta ctcttcgccg acagattgcg ggccagcaaa tttgggcggt
    20881 aactgctctc ttctaccgcc tgaaacacgc gatctttagt ctcctggctg acgtagccat
    20941 tacctgaaag cacgcgggaa acggtcgctt ttgaaacccc ggcgcgcttc gccacttcca
    21001 gcatcgtcgt catcattttc atccctttac acgcaatcaa cgcagtgtac tgcaccgttt
    21061 gccgattgtc cttgcgcaat cggctccagc ggataagacg aggtcgccgt gccggttttg
    21121 atgacttttt tgataaaggt aaacatggcg attccttatt tcagcggcga gtttttacgc
    21181 tcaatgctgt aacgctcgag ttctttgtac ggcaccactt tgctcttctt cttacgcaca
    21241 tcgactacgg tcatgcggtc ggtacaggag tagcaagggt ccaggctacc gataatcagc
    21301 ggcgcatagg aaacggtatt gccacgcagc atgtagcgca gggtcggcca gttcgcgtag
    21361 gtcgcggcac ggcagcgcca gcggtacagc ttctggttgt cgccggtcat gctccagtgg
    21421 atatcgtcgc cgcgcggcgc ttcggcaaag cccagcgcga aacggtgcgg aatgtaggta
    21481 aagccttcca ccatcagcgg accgcccggc aggttatcca gaccgtagtc gatcatattc
    21541 agcgcggtat agacctcgtt gattcgcact ttcagacggg aaataacgtc gcagccctgc
    21601 tcgctgtgga cttccattgg cagcaggcca taaccgacaa acgggtgatc ggcgcgagta
    21661 tcgcgggcga gaccgctggc gcgaaccatc gggccaacgt tactgaaatc gcgggcgatt
    21721 tccgggtcca gacgaccaat gccgacggtg cgttgttcca tgttcggcgt actcagcagc
    21781 acatccacca gctcctgcac ttcacgacgc atttgttgcg ccagctggcg ggtctggatc
    21841 atgtcatctt tcagcagatc gcgacgaatc ccgccgatca ggttcaggcc gtaggtttta
    21901 cgcgcaccgg taaggatctc tgccattttc atggaggttt cacgcacgcg gaagaactgc
    21961 ataaagccgg agtcgaagcc ggtaaagtga caggcgaggc cgaggttgag cagatgcggg
    22021 tgcaggcgtt ccacctccag cagaatggcg cggatcatct gcgcgcgttc tggcacctga
    22081 atacccatcg cgttttccac cgacgtggtg taggcggtgc tgtgggcaaa gccgcagatc
    22141 ccgcacacac ggtcagagag gaaggtcact tcgttataac ccatgcgggt ttccgccagt
    22201 ttttccatac cgcgatggac gtagaacaga cggtagtcgg cgtcgataat gttttcgcca
    22261 tcgacgaaca gacggaagtg acccggttcg tcagaagtga cgtgcagcgg accaatcggc
    22321 acgacgttgt ttttcttgtc gcccagttcg ttgatgaact cgtaggtttc agcatcggta
    22381 gtcggtgccg gacgctgacg ataatccatg ctgtctttac gcagcggata aagttcatcc
    22441 ggccagtcat ccggcagcac cagacgacgt tcatccggca gaccaaccgg aatcaaaccg
    22501 tacatatcgc gcacttcgcg ctcgccccac accgccgccg gaacgcgcgg cgtcacggac
    22561 ggatactccg gtttgttggc gtcaacttcg acgcgaaccg ttacccaaca cttggtgccc
    22621 ttctccatcg acagcacgta ataaacggcg taatgaccat tcagtttgcg ttcgtcgtta
    22681 ccaaacagca ccgacagcca gccaccctgc ttgtagtaga gaaactccac cacttccggc
    22741 agatagttca cctttacggt gacagtcagc tgatctttgg tctgccaggc gtggtccagc
    22801 acgacgccta gaaatgcctc attcagcgcg gcgagataat gttgacctaa tttttcttca
    22861 gacatgctca aactctcttt aatcacgccg ccagcaagga gacgaacgct aaaaatgcaa
    22921 agccaaaccc agcccaggta atgcgcggag taatatcaag acgcagacgg gccatgctgt
    22981 tttcgaacag cgcgataacc aggacgccga ccaccagttt gacgatggca atcaccagcg
    23041 ccagcagcag tccaccgacg gtgaaggttt ccatttgtcc ccacggaata aacaccccga
    23101 cgaacatctg caacaccacc agctgtttca ggctgatacc ccatttcatg acgccaaagc
    23161 cgctaccgct gtattcggag agcggacctt cctgcaactc ctgctcggct tccgccaggt
    23221 cgaacggcag tttgcccatt tcgataaaag tggcgaacgc acaggcgcag agcgccagca
    23281 ccagcgggat gctttggctc agcggccagt gataaacggt gtcggtaatg ttgctgatgt
    23341 tggtggaacc ggcaacctgt gcggcaaccc acagaccaag cagcaacatc ggttcgacca
    23401 gcacgccaag catcgcttca cggctcgcac cgatagcggt aaacgggcta ccggtatcca
    23461 gaccggaaat ggcaaagaag aaacgcgcga tggcgaagag atacagtaag gtgatcaaat
    23521 cgcccagttg cggcagcgga gaaccgacgg tcaccaccgg cagcgcagta gcgatagtca
    23581 gcatgacgcc caccatcaca tacggcgtca ggcggaacat ccagccagag gcatccgggc
    23641 caacgctctg gcggcccagc agtttgataa tgtcgcgata ctcctgcaac acgcccggcc
    23701 cgcgacggtt atgcagacgg gcgcgcgcca cgcgggtaat accggagagc agcggcgcaa
    23761 cggcaaataa caccagcgcc tgaattaacg gatataaaac actcattctc aggctcctcg
    23821 tgaaacaata atcaccacca gtaccgccag ttcaaccagc gccatccggc ggaacagcaa
    23881 cgcactacct tcgcactgcc agcccggcac tagcgacacc ggattcagcc atttgcgcag
    23941 tttcagcact ggcgcaaacg cctgtttaac cggcatggca aaaccgtgag cggtaatcac
    24001 cattgatttt tcatggtcgt agccgcacac ccaggccgca ccgcgggaac gtgacggcag
    24061 gcggtcgcct ttgcaaatcg ccataatgat gaatggcagc agcgggcagg caatcagcag
    24121 caatgtgatc attggttgag aaacggtggt gttagcaggc tccagaggca gaggtacagc
    24181 agcagagagc atcggcagca gccacggcgc agcaacaccg ccaattacgc agcaaatcgc
    24241 cagtgccact acgcttacgc tcatcaggag cggcgcacag gtggcgtttt cggcttcttt
    24301 ggtacgcggc gcgccgagga acgtgacgcc atagactttc gccatacaca tcaccgccag
    24361 cgcaccggta attgccagac ccacagcgag cagcggcccc agcagacggg caacaaacgc
    24421 gccgctattg ctcagtttga agaaggattg atagataacc cattccccgg caaacccgtt
    24481 cagtggcggc agcgcagcca ttgccatcaa cccgactaac atggcgatgg agataaccgg
    24541 cattttcttg ccaataccac cgagtttttc gatatcgcga tgaccggtac ggaaccagac
    24601 gctccccgcc ccgaggaaca gtacgctttt gaacaggcta tggttaagca gatggtacag
    24661 accaccgacc aggccaagag caatcagcgc cggttgttcg agcgcgatac ccgtta
//
LOCUS       DAZPCS010000043        24246 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZPCS010000043 DAZPCS010000000
VERSION     DAZPCS010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24246
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.043"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            503..2431
                     /gene="thrS"
                     /locus_tag="ACHUQ7_001763"
     CDS             503..2431
                     /gene="thrS"
                     /locus_tag="ACHUQ7_001763"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFR1135199.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2435..2977
                     /gene="infC"
                     /locus_tag="ACHUQ7_001764"
     CDS             2435..2977
                     /gene="infC"
                     /locus_tag="ACHUQ7_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFR1135200.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3074..3271
                     /gene="rpmI"
                     /locus_tag="ACHUQ7_001765"
     CDS             3074..3271
                     /gene="rpmI"
                     /locus_tag="ACHUQ7_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFR1135201.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3324..3680
                     /gene="rplT"
                     /locus_tag="ACHUQ7_001766"
     CDS             3324..3680
                     /gene="rplT"
                     /locus_tag="ACHUQ7_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFR1135202.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTTLVEKAKAALA"
     misc_feature    3797..3921
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            3803..3847
                     /gene="pheM"
                     /locus_tag="ACHUQ7_001767"
     CDS             3803..3847
                     /gene="pheM"
                     /locus_tag="ACHUQ7_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFR1135203.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4130..5113
                     /gene="pheS"
                     /locus_tag="ACHUQ7_001768"
     CDS             4130..5113
                     /gene="pheS"
                     /locus_tag="ACHUQ7_001768"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFR1135204.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVSIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5128..7515
                     /gene="pheT"
                     /locus_tag="ACHUQ7_001769"
     CDS             5128..7515
                     /gene="pheT"
                     /locus_tag="ACHUQ7_001769"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFR1135205.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPVLQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7520..7819
                     /gene="ihfA"
                     /locus_tag="ACHUQ7_001770"
     CDS             7520..7819
                     /gene="ihfA"
                     /locus_tag="ACHUQ7_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFR1135206.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            7920..8900
                     /gene="btuC"
                     /locus_tag="ACHUQ7_001771"
     CDS             7920..8900
                     /gene="btuC"
                     /locus_tag="ACHUQ7_001771"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFR1135207.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPMWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            8963..9514
                     /gene="btuE"
                     /locus_tag="ACHUQ7_001772"
     CDS             8963..9514
                     /gene="btuE"
                     /locus_tag="ACHUQ7_001772"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFR1135208.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTTVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9514..10263
                     /gene="btuD"
                     /locus_tag="ACHUQ7_001773"
     CDS             9514..10263
                     /gene="btuD"
                     /locus_tag="ACHUQ7_001773"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFR1135209.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10341..10805
                     /locus_tag="ACHUQ7_001774"
     CDS             10341..10805
                     /locus_tag="ACHUQ7_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFR1135210.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLHLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11052..11765
                     /gene="ydiV"
                     /locus_tag="ACHUQ7_001775"
     CDS             11052..11765
                     /gene="ydiV"
                     /locus_tag="ACHUQ7_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFR1135211.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLEKGFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            11829..13265
                     /gene="selO"
                     /locus_tag="ACHUQ7_001776"
     CDS             11829..13265
                     /gene="selO"
                     /locus_tag="ACHUQ7_001776"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFR1135212.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFVVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLREEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13269..13460)
                     /gene="hemP"
                     /locus_tag="ACHUQ7_001777"
     CDS             complement(13269..13460)
                     /gene="hemP"
                     /locus_tag="ACHUQ7_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFR1135213.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13592..14638)
                     /gene="aroH"
                     /locus_tag="ACHUQ7_001778"
     CDS             complement(13592..14638)
                     /gene="aroH"
                     /locus_tag="ACHUQ7_001778"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFR1135214.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLAFAVDTRF"
     gene            complement(14795..15628)
                     /gene="ppsR"
                     /locus_tag="ACHUQ7_001779"
     CDS             complement(14795..15628)
                     /gene="ppsR"
                     /locus_tag="ACHUQ7_001779"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFR1135215.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            15960..18338
                     /gene="ppsA"
                     /locus_tag="ACHUQ7_001780"
     CDS             15960..18338
                     /gene="ppsA"
                     /locus_tag="ACHUQ7_001780"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFR1135216.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18395..20041)
                     /gene="fadK"
                     /locus_tag="ACHUQ7_001781"
     CDS             complement(18395..20041)
                     /gene="fadK"
                     /locus_tag="ACHUQ7_001781"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HFR1135217.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNIPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWLDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRSIKLLSVY
                     GSTESSPHAVVNLDNSLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEMAAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASDKIQKFLLRKDIIRRLTQDVCEEIE"
     gene            complement(20098..20391)
                     /locus_tag="ACHUQ7_001782"
     CDS             complement(20098..20391)
                     /locus_tag="ACHUQ7_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFR1135218.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(20388..21677)
                     /locus_tag="ACHUQ7_001783"
     CDS             complement(20388..21677)
                     /locus_tag="ACHUQ7_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR1135219.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(21733..22671)
                     /locus_tag="ACHUQ7_001784"
     CDS             complement(21733..22671)
                     /locus_tag="ACHUQ7_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFR1135220.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGVKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(22691..23455)
                     /locus_tag="ACHUQ7_001785"
     CDS             complement(22691..23455)
                     /locus_tag="ACHUQ7_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFR1135221.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            23774..>24246
                     /locus_tag="ACHUQ7_001786"
     CDS             23774..>24246
                     /locus_tag="ACHUQ7_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFR1135222.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDALAYTLA"
BASE COUNT         5983 a         5897 c         6390 g         5976 t
ORIGIN      
        1 tctggaggtt ttgaggatga tgattaatta ttatcgccag aacgaaatgt gcacgaaaaa
       61 ttgttataag aattgtgtca cttttatcat tgtgatatga ccacttcgtc attaatctca
      121 tgagcgttta aaatactacc gccaaatcac gcaacgaact ttgctggatt cagtttatgc
      181 tgctgtaaat ccgctcgcag taaacctttc agacgcaccg tgatgttatc agtggttctt
      241 taagcgtttt gctggtgtac tcactacaaa cgaattgcga atcgaatcaa tgtgaaacgg
      301 aaaggtacaa tctccctttt tttagtgtta acatcgttca accgggtgga aggtgagagg
      361 ttaatgtctt tcatttgtgt acctgattgc gaaccaattt agcatttgtt ggctagatgg
      421 tttcgcaatg aactgttaat aaacaaattt ttctttgtat gtgatctttc gtgtgggtca
      481 ccactgcaaa taaggatata aaatgcctgt tataactctt cctgatggca gccaacgcca
      541 ttacgaccac gctgtaagcc ccatggatgt tgcgctggac attggtccag gtctggcgaa
      601 agcctgtatc gcagggcgcg ttaatggcga actggttgat gcttgcgatc tgattgaaaa
      661 cgacgcacaa ctgtcgatca ttaccgccaa agacgaagaa ggtctggaga tcattcgtca
      721 ctcctgtgcg cacctgttag ggcatgcgat taaacaactt tggccgcata ccaaaatggc
      781 aatcggcccg gttattgaca acggttttta ttacgacgtt gatcttgacc gcacgttaac
      841 ccaggaagat gtcgaagcac tcgagaagcg gatgcatgag cttgctgaga aaaactacga
      901 cgtcattaag aagaaagtca gctggcacga agcgcgtgaa actttcgcca accgtgggga
      961 gagctacaaa gtttccattc ttgacgaaaa catcgcccat gatgacaagc caggtctgta
     1021 cttccatgaa gaatatgtcg atatgtgccg cggtccgcac gtaccgaaca tgcgtttctg
     1081 ccatcatttc aaactaatga aaacggcagg ggcttactgg cgtggcgaca gcaacaacaa
     1141 aatgttgcaa cgtatttacg gtacggcgtg ggcagacaaa aaagcactta acgcttacct
     1201 gcagcgcctg gaagaagccg cgaaacgcga ccaccgtaaa atcggtaaac agctcgacct
     1261 gtaccatatg caggaagaag cgccgggtat ggtattctgg cacaacgacg gctggaccat
     1321 cttccgtgaa ctggaagtgt ttgttcgttc taaactgaaa gagtaccagt atcaggaagt
     1381 taaaggtccg ttcatgatgg accgtgtcct gtgggaaaaa accggtcact gggacaacta
     1441 caaagatgca atgttcacca catcttctga gaaccgtgaa tactgcatta agccgatgaa
     1501 ctgcccgggt cacgtacaaa ttttcaacca ggggctgaag tcttatcgcg atctgccgct
     1561 gcgtatggcc gagtttggta gctgccaccg taacgagccg tcaggttcgc tgcatggcct
     1621 gatgcgcgtg cgtggattta cccaggatga cgcgcatatc ttctgtactg aagaacaaat
     1681 tcgcgatgaa gttaacggat gtatccgttt agtctatgat atgtacagca cttttggctt
     1741 cgagaagatc gtcgtcaaac tctccactcg tcctgaaaaa cgtattggca gcgacgaaat
     1801 gtgggatcgt gctgaggcgg acctggcggt tgcgctggaa gaaaacaaca tcccgtttga
     1861 atatcaactg ggtgaaggcg ctttctacgg tccgaaaatt gaatttaccc tgtatgactg
     1921 cctcgatcgt gcatggcagt gcggtacagt acagctggac ttctccttgc cgtctcgtct
     1981 gagcgcctcc tatgtgggcg aagacaacga gcgtaaggta ccggtaatga ttcaccgcgc
     2041 aattctgggg tcgatggagc gtttcatcgg tatcctgacc gaagaattcg ctggtttctt
     2101 cccgacctgg cttgcgccag ttcaggttgt tatcatgaat attaccgatt cacagtctga
     2161 atacgttaac gaattgacgc aaaaactatc aaatgcgggc attcgtgtta aagcagactt
     2221 gagaaatgag aagattggct ttaaaatccg cgagcacact ttgcgtcgcg tcccatatat
     2281 gctggtctgt ggtgataaag aggtggaatc aggcaaagtt gccgttcgca cccgccgtgg
     2341 taaagacctg ggaagcatgg acgtaaatga agtgatcgag aagctgcaac aagagattcg
     2401 cagccgcagt cttaaacaat tggaggaata aggtattaaa ggcggaaaac gagttcaaac
     2461 ggcgcgccct aaccgtatca atggcgaaat tcgcgcccag gaagttcgct taacaggtct
     2521 ggaaggcgag cagcttggta ttgtgagtct gagagaagct ctggagaaag cagaagaagc
     2581 cggagtagac ttagtcgaga tcagccctaa cgccgagccg ccggtttgtc gtataatgga
     2641 ttacggcaaa ttcctctatg aaaagagcaa gtcttctaag gaacagaaga aaaagcaaaa
     2701 agttatccag gttaaggaaa ttaaattccg tcctagtaca gatgaaggcg actatcaggt
     2761 aaaactccgc agcctgattc gctttctcga agagggtgat aaagccaaaa tcacgctgcg
     2821 tttccgcggt cgtgagatgg cgcaccagca aatcggtatg gaagtgctta atcgcgtgaa
     2881 agacgatttg caagaactgg cagtagtcga atccttccca acgaagatcg aaggccgcca
     2941 gatgatcatg gtgctcgctc ctaagaagaa acagtaaggc cttcaagtag cactatctgt
     3001 ggagcctttg ggcttcacag gcattgttcg cctttgtttc gtttattaac aatgcgaagt
     3061 ggaagttatt aaaatgccaa aaattaagac cgtacgcggt gctgctaagc gcttcaaaaa
     3121 aaccggtaaa ggtggtttta agcacaagca cgctaacctg cgtcacattc tgactaaaaa
     3181 agcgaccaaa cgtaaacgtc acctgcgtcc gaaagccatg gtttccaaag gcgatctggg
     3241 cctggtaatc gcgtgcctgc cgtacgcata agccgttaac gtttttaact ttttaattag
     3301 aatatagata caggagagca catatggctc gcgtaaaacg tggtgttatt gcacgcgcac
     3361 gtcacaagaa aattttgaaa caagctaaag gctactacgg tgcgcgttct cgcgtatacc
     3421 gcgttgcctt ccaggctgtt atcaaagctg gtcagtatgc ttaccgtgac cgtcgtcaac
     3481 gtaagcgtca gttccgtcaa ctgtggattg cgcgtatcaa cgcagcagca cgtcagaacg
     3541 gtatttctta cagcaaattc atcaatggcc tgaaaaaagc ctctgttgaa atcgaccgta
     3601 agatcctggc tgatatcgca gtattcgaca aagtagcgtt caccactctg gttgaaaaag
     3661 cgaaagcagc tctggcataa gccagttgaa agagggagct agtctccctc ttttcgtttc
     3721 aacgccatca aaacattgac ttttatcgcc gtagcctttt caataaaggt cttttgaaga
     3781 gtaccaaaag gtaacgcaag caatgaatgc tgctattttc cgcttctttt tttactttag
     3841 cacctgaatc caggaggcta gcgcgtgaga agagaaacgg aaaacagcgc ctgaaagcct
     3901 cccagtggag gctttttttg tatgcgcgtt ttgaaaaata aaaagccagt ctatcaggcg
     3961 taattgtcgc agccagtctg gacacggcca gtgcggagaa accggagcgt acttaagtac
     4021 gtgaggattt cgagcactgc ccgggaccaa aatggcaagt aaaatagcct gatgggatag
     4081 gctctaagtc caacgaacca gtgtcaccac tgacacaatg aggaaaacca tgtcacatct
     4141 cgcagaactg gttgccagtg cgaaggcggc cattagccag gcgtcagatg ttgccgcgtt
     4201 agataatgtg cgcgtcgaat atttgggtaa aaaagggcac ttaacccttc agatgacgac
     4261 cctgcgtgag ctgccgccag aagagcgtcc ggcagctggt gcggttatca acgaagcgaa
     4321 agagcaggtt cagcaggcgc tgaatgcgcg taaagcggaa ctggaaagcg ctgcactgaa
     4381 tgcgcgtctg gcggcggaaa cgattgatgt ctctctgcca ggtcgtcgca ttgaaaacgg
     4441 cggtctgcat ccggttaccc gtaccatcga ccgtatcgaa agtttcttcg gtgagcttgg
     4501 ctttaccgtg gcaaccgggc cggaaatcga agacgattat cataacttcg atgctctgaa
     4561 cattcctggt caccacccgg cgcgcgctga ccacgacact ttctggtttg acgctacccg
     4621 cctgctgcgt acccagacct ctggcgtaca gatccgcacc atgaaagccc agcagccacc
     4681 gattcgtatc atcgcgcctg gccgtgttta tcgtaacgac tacgaccaga ctcacacgcc
     4741 gatgttccat cagatggaag gtctgattgt tgataccaac atcagcttta ccaacctgaa
     4801 aggcacgctg cacgacttcc tgcgtaactt ctttgaggaa gatttgcaga ttcgcttccg
     4861 tccttcctac ttcccgttta ccgagccttc tgcagaagtt gacgtcatgg gtaaaaacgg
     4921 taaatggctg gaagtgctgg gctgcgggat ggtgcatccg aacgtgctgc gtaacgttag
     4981 catcgacccg gaagtttact ctggtttcgc cttcgggatg gggatggagc gtctgactat
     5041 gttgcgttac ggcgtcaccg acctgcgttc attcttcgaa aacgatctgc gtttcctcaa
     5101 acagtttaaa taaggcagga atagattatg aaattcagtg aactgtggtt acgcgaatgg
     5161 gtgaacccgg cgatcgatag cgatacgctg gcgaatcaaa tcactatggc gggcctggaa
     5221 gttgacggtg tagaaccggt tgccggtagc ttccacggcg tggtcgttgg tgaagtggtt
     5281 gagtgtgcgc agcatccgaa cgctgacaaa ctgcgtgtga caaaagtgaa tgtcggcggc
     5341 gatcgcctgc tggacatcgt ctgcggtgcg ccaaactgcc gtcagggcct gcgtgtggcg
     5401 gtagctacca ttggtgctgt tctgccgggt gatttcaaaa ttaaagcggc gaaactgcgt
     5461 ggcgaaccgt ctgaagggat gctgtgctcc ttttctgagc tgggaatttc tgacgatcac
     5521 agtggcatta tcgaactgcc tgcggatgcg ccgattggca ccgacatccg cgaatacctg
     5581 aaactcgatg ataacaccat cgaaatcagc gtgacaccaa accgtgccga ctgcttaggt
     5641 atcattggtg ttgcgcgtga cgttgccgtg ctgaatcagc tgccgctggt tgaaccggaa
     5701 atcgttccgg ttggtgcgac tatcgacgac acgttgccga ttacagtcga agcgccggaa
     5761 gcctgcccgc gctatcttgg ccgtgtggta aaaggcatta acgttaaagc gccaactccg
     5821 ctgtggatga aagaaaaact gcgtcgttgc gggatccgtt ctatcgatgc agttgttgac
     5881 gtcaccaact atgtactgct cgaactgggc cagccgatgc acgctttcga taaagatcgc
     5941 attgaaggcg gcattgtggt gcggatggca aaagagggcg aaacgctggt gctgcttgat
     6001 ggcactgaag cgaagctgaa tgctgacact ctggtcatcg ccgaccacaa caaggcgctg
     6061 gcgatgggcg gcatctttgg tggcgaacac tctggcgtga atgacgaaac gcaaaacgtc
     6121 ttgctggaat gcgctttctt cagcccgctg tctatcaccg gtcgtgctcg tcgtcatggc
     6181 ctgcatactg atgcgtctca ccgttatgag cgtggcgttg atccggtact gcagcacaaa
     6241 gcgatggaac gtgcgacccg tctgctgatc gacatctgcg gtggtgaggc tggcccggta
     6301 attgatatca ccaacgaagc aacgctgccg aagcgtgcaa ccatcactct acgtcgtagc
     6361 aaactggatc gcctgatagg ccatcatatt gctgatgagc aggtaactga cattctgcgt
     6421 cgtctcggct gcgaagtgac cgaaggcaaa gacgaatggc aggcagttgc gccgagctgg
     6481 cgtttcgata tggagattga agaagatctg gtcgaagaag tcgcgcgtgt ttacggctac
     6541 aacaacatcc cggatgagcc ggtacaggca agcctgatta tgggtactca ccgtgaagct
     6601 gacctgtcgc tcaagcgcgt gaaaacgctg ctcaacgaca aaggctatca ggaagtgatc
     6661 acctacagct tcgttgatcc gaaagtgcag cagatgatcc atccaggcgt tgaagcctta
     6721 ctgctggcaa gcccgatctc tgttgaaatg tcagcaatgc gtctttctct gtggaccggc
     6781 ctgctggcaa ccgtggtgta caaccagaac cgtcagcaga accgtgtgcg cattttcgaa
     6841 agcggtctgc gtttcgtacc agatactcag gcaccgttgg gcattcgtca ggatctgatg
     6901 ttagccggtg tgatttgcgg taaccgttac gaagagcact ggaacctggc aaaagagacc
     6961 gttgatttct atgatttgaa aggcgacctt gaatccgttc tcgacctgac cggtaaactg
     7021 aatgaggttg agttccgtgc agaagcgaat ccggcactgc atccggggca atccgcagcg
     7081 atttatctga aaggtgaacg tattggtttt gttggagttg ttcatcctga actggaacgt
     7141 aaactggatc ttaacggtcg cactctggtg ttcgaactgg agtggaacaa gctcgcagac
     7201 cgcgtggtgc ctcaggcgcg cgagatttct cgcttcccgg cgaaccgtcg tgacatcgcg
     7261 gtggtggtcg cagaaaacgt tcccgcagcg gatattttat ccgaatgtaa gaaagttggc
     7321 gtaaatcagg tagttggcgt aaacttattt gacgtgtacc gcggtaaggg tgttgcggag
     7381 gggtataaga gcctcgccat aagcctgatc ctgcaagata ccagccgtac actcgaagaa
     7441 gaggagattg ccgctaccgt cgccaaatgt gtagaggcat taaaagagcg attccaggca
     7501 tcattgaggg attgaaccta tggcgcttac aaaagctgaa atgtcagaat atctgtttga
     7561 taagcttggg cttagcaagc gggatgccaa agaactggtt gaactgtttt tcgaagagat
     7621 ccgtcgcgct ctggaaaacg gcgaacaggt gaaactctct ggttttggta acttcgatct
     7681 gcgtgataag aatcaacgcc cgggacgtaa cccgaaaacg ggcgaggata ttcccattac
     7741 agcacggcgc gtggtgacct tcagacccgg gcagaagtta aaaagccggg tcgaaaacgc
     7801 ttcgcccaaa gacgagtaat ctgatctaac taaaaaggcc gctatgcggc cttttttctt
     7861 ttcactgtcg aagagtcacc gtaaaatcaa cgccatgaca cttcagcaga acggatacca
     7921 tgctgacact tgcccgccaa caacagcgac aaaatattcg ctggttatta tgcctgtcag
     7981 ttttgatgct gctggcgctt ctcttaagcc tttgcgccgg tgaacaatgg atttcgccag
     8041 gtgactggtt tactcctcgt ggcgaactgt tcgtctggca aattcgcctg ccacgtacgc
     8101 tggctgtatt gctggttggt gcggcgctgg ctatatccgg cgctgtaatg caggcgttgt
     8161 ttgaaaatcc tctggcagaa cctggactac ttggcgtctc taacggcgca ggcgtggggc
     8221 ttatcgccgc ggtattgctt gggcaagggc aactccccaa ctgggcgcta gggctgtgtg
     8281 cgattgctgg cgcgcttatc atcactttaa tactcttacg tttcgcccgt cgtcatcttt
     8341 cgaccagtcg gttattgctg gctggcgttg cattagggat tatctgcagc gcactaatga
     8401 cgtgggctat ctacttttcc acctctgttg atttacgtca gctgatgtac tggatgatgg
     8461 gcggttttgg cggcgtagac tggcggcaaa gctggctgat gctggcattg atccccgtgt
     8521 tgttgtggat ctgttgtcag tccaggccga tgaatatgtt agcgcttggc gagatctcgg
     8581 cgcggcaact gggtttaccc atgtggttct ggcgcaatgt gctggtggca gcgaccggct
     8641 ggatggttgg cgtcagtgtg gcgctggcgg gtgctatcgg ctttattggt ctggtgatcc
     8701 cacatattct tcggttgtgt ggtttaaccg atcatcgcgt attacttccc ggctgcgcgc
     8761 tggcaggggc gagcgcattg ctgctggccg atattgtagc gcgcctggca ttagctgccg
     8821 cagagctgcc tattggcgtg gtcaccgcaa cgttgggtgc gccggtgttt atctggttat
     8881 tgttaaaagc aggacgttag ccgcaaaaag acggtctatg attaaaagct aagattttta
     8941 ctgaccacac ctaggagaaa cgatgcaaga ttccattctg acgaccgtag tgaaagatat
     9001 cgacggtgaa gtgaccacgc tggagaagta cgccggtaat gtgctgttaa ttgtcaatgt
     9061 cgcctcaaag tgtggcttaa cgccgcaata tgagcagttg gagaatattc aaaaagcctg
     9121 ggccgatcga ggttttgtgg tgctgggatt cccgtgcaac cagtttctgg aacaagaacc
     9181 gggcagcgat gaagagatta aaacttactg taccaccaca tggggggtga cgttcccgat
     9241 gttcagtaag attgaagtta atggcgaagg acgccatccg ctgtatcaaa aattgattgc
     9301 cgcagcgccg accacagtcg cgccggaaga gagcggattc tatgcccgta tggtcagcaa
     9361 aggccgtgca ccgctgtacc cggatgatat tttatggaat tttgaaaaat tcctggttgg
     9421 cagggacgga aaagtcatcc agcgtttttc cccggatatg acgccggaag atcccattgt
     9481 gatggaaagc attaaactgg cgttggcaaa ataatgtcta ttgtgatgca gttacaggat
     9541 gttgcggaat ctacccgcct ggggccgctt tcaggcgagg ttcgggctgg ggaaatcctg
     9601 cacctggtgg ggccgaatgg cgcgggtaag agtaccttac tggcgcgaat ggccggaatg
     9661 accagcggta agggaagcat tcagttcgcg gggcaaccac tggaagcatg gtccgcaaca
     9721 aaactcgcgc tgcatcgcgc ctatctttca caacagcaga cgccgccgtt tgcaatgccg
     9781 gtctggcact acctgacact gcatcagcac gataaaacgc gtaccgaact actgaatgat
     9841 gtcgcagggg cgctggctct tgatgacaaa ctcggtcgta gcaccaatca actttccggc
     9901 ggtgaatggc aacgcgtacg tcttgctgcg gtggtgttgc aaatcacacc acaagccaat
     9961 cccgcaggcc aattgctgct tcttgatgag ccgatgaaca gtcttgatgt tgcgcaacaa
    10021 agtgcgttag acaaaattct gagcgcgctg tgtcagcaag gactggcgat tgtgatgagc
    10081 agtcacgatc tcaaccacac gttgcgtcat gcgcatcggg cgtggttgct aaaaggtgga
    10141 aaaatgctgg ccagtggacg cagagaagag gtgctcacgc cgccaaatct ggcgcaggcc
    10201 tatgggatga attttcgccg tctggatatc gaaggtcaca gaatgctgat ttcgaccatc
    10261 tgatttcatt agtatcccga aaatcgggta attacgacgc taaattaatg caagaataaa
    10321 aaacagagga accgtacgga atgcgtttct gccttatttt gatcacagca ctgtttctgg
    10381 ccgggtgtag ccaccataaa gcaccgccgc caaatgccag actttctgat tcgattaccg
    10441 ttattgccgg tttgaacgac cagctacaaa gctggcatgg cacgccgtat cgttatggtg
    10501 gcatgacgcg gcgcggtgtg gactgttcgg gatttgtggt tgtgacgatg cgcgatcgtt
    10561 tcgatttgca tctgccccgt gaaaccaaac aacaagcctc tattggcacg caaattgata
    10621 aagacgagtt gctgcctggt gacctggtct ttttcaaaac gggttccgga caaaatggtt
    10681 tgcatgtagg tatttacgat accaacaacc aatttatcca cgcctctacc agcaagggag
    10741 tgatgcgttc ctcacttgat aatgtctatt ggcagaaaaa tttctggcag gcgagacgaa
    10801 tctagtatgt cgaaaaattg acggcgatga tacatcgccg tcaatttact cctgaataca
    10861 gtagtatttt gcagacaaaa attccgtaaa tggattattc gtttataatc aggataatct
    10921 aaaaaaaggc cccctgaaaa agtggttaac atggaacaaa tgaaagttac tccttctgta
    10981 attgttatgt cttatggcct catccctttt agccggatac tgaaaaacat ccttcgagag
    11041 ggacggttac catgaagatt tttttggaga atctttatca ttctgattgc tatttcctgc
    11101 cgataagaga taaccagcaa ctccttgtcg gcgttgagtt aattacccat ttctccagtg
    11161 aagatggcac ggttcgtatc ccgacgagtc gggttatagc gcaacttacc gaggagcaac
    11221 attggcaatt attttcagag cagttggaat tactaaaatc atgccaacac ttttttattc
    11281 aacataaact ctttgcctgg cttaatttaa cgccacaagt ggctacatta ttattagaca
    11341 gggataatta cgcaggcgaa ctattaaaat atccgtttat tgagctgttg attaatgaga
    11401 attacccgca tctcaatgaa ggaaaagata atcgcgatct tttgtcctta tcgcaaatgt
    11461 atccccttgt gttaggcaat ttgggagcag gtaatagcac aatgaaagcg gtttttgatg
    11521 gtcttttcac ccgcgtaatg ctggagaaag gttttattca gcaacaaatc acgcatcgct
    11581 ccttcgaacc gtttattcgt gcgatccagg cgcaaatttc cccttgttgc aattgcatca
    11641 tcgcgggggg cattgatacg ccagaaatat tggcacagat tacccccttc gatttccatg
    11701 ctctccaggg atgcctgtgg cctgccgttc ctataaatca gataacgacg ttggttcagc
    11761 gataaccctc ctgtttgctg gtgtttaaga cgagagtaac cgtctacact atcaaacaga
    11821 taggatctat gaccctgtct tttattaccc gctggcgcga tgaattgcca gaaacctata
    11881 cagcactttc ccctacgcct ttaaataatg cccggctgat ttggcataat accgaactcg
    11941 ctaacacgct gagtattcca tcgtcgctgt ttaaaaatgc cgcaggtgtc tggggcggcg
    12001 aaaccctact gcctggtatg tcaccactgg cacaggttta cagtggtcat cagttcgtcg
    12061 tctgggcggg ccaactgggt gatgggcgcg gcattttact tggcgaacaa ctgcttgctg
    12121 atggcactac aatggactgg catctgaaag gtgctggcct gacgccttat tcgcgaatgg
    12181 gtgatggacg ggcggtttta cgttcgacga tacgagaaag tctcgccagt gaggcgatgc
    12241 attatctggg cattccgacg acccgcgcgt taagtatcgt caccagcgat tcgccagtgt
    12301 atcgggaaac ggtggagcca ggcgcgatgc tgatgcgtgt ggcaccaagt catctgcgct
    12361 ttggtcattt cgaacatttt tactatcgcc gcgagccgga aaaggttcgt cagttggctg
    12421 actttgccat tcgtcattac tggtcacatc ttgaagacga tgaggacaaa taccgtctct
    12481 ggtttaacga tgttgtcgca cgtaccgcgt cgttaattgc ccaatggcag acggtcggct
    12541 ttgcacatgg ggtgatgaat accgacaaca tgtcgctgct gggactgacg cttgattacg
    12601 ggccgtttgg ttttcttgat gattacgaac ccggttttat ttgtaatcac tcggatcatc
    12661 aagggcgtta tagctttgat aatcaacctg ctgtcgcgtt gtggaattta cagcgtctgg
    12721 cgcagacatt gtcaccattt gttgccgtag atgccctgaa tgaggccctg gacagctatc
    12781 agcaggtttt gttgacgcat tatggacaac ggatgcggca gaaactgggc ttcatgacgg
    12841 agcaaaaaga ggataacgcg ctactgaatg aattattcag tctgatggcg cgagagcgca
    12901 gcgattatac ccgcacattc cgcatgctga gtctgaccga gcagcacagc gcggcgtcac
    12961 cgctacgtga ggagtttatt gatcgtgcgg catttgatga ctggtttgcc cgttatcggg
    13021 ggcgtttgca acaagacgag gttagcgata gtgagcgtca gcaactgatg caaagcgtta
    13081 acccagctct ggtattgcgc aactggttgg cgcaacgggc gattgaagcg gcagaaaagg
    13141 gtgatatgat ggaattgcac cgcctgcatg aggcgttgcg aaatcctttc agcgacagag
    13201 atgatgacta tgtcagtcgt ccacctgact ggggtaaacg gctggaagtc agctgctcga
    13261 gttaaaggct acttagttaa cagcagcttg ccagcctgag ttttacggag cagatattct
    13321 tgcccgtcat gatcgataat cagtttgcca tccggaccta acagtgtctg gctggtaatt
    13381 cgccggatgg gctgcgggga agcggtcttg tgattggcat ccgtttcagg cgtgagtttt
    13441 ctgctatccg tataacgcat aaaaattaat aataattgat ataacaatga ttcctattat
    13501 caatattatg gcagaccaga gcaagcagta atgtgttgag aagacaacaa gttgcgcaga
    13561 aatgcccatc ccatcaggaa tgggcacgca ttcagaagcg ggtatctacc gcaaaggcga
    13621 gtttttcgac caggcgttcg gtatcttccc agcccagaca cgggtcggta atggattgac
    13681 cgtaagtgag cggctgaccg ccgacgattt tttgcgttcc ttcgcgcagg aaactttccg
    13741 ccataattcc agcaatctcc gtagagccat tgcggatttg ctgacaaata tcctcacaaa
    13801 cttctaactg gcgacggtgc tgcttctggc agttaccgtg gctgaaatcc accaccagat
    13861 gttcaggtaa atcaaactcg tgcagcgtat cgcaggctgc ggcgatatca tcggcatgat
    13921 aattcggttt tttgccgcca cgcataataa tgtggccata cgggttgccg ctggtctgat
    13981 agatggtcat ctgaccattt ttgtctggcg agaggaacat atggctggcg cgggctgcgc
    14041 ggatagcatc cacggcaatc cgcgtgttgc catcggtacc atttttaaaa cctaccggac
    14101 aggagagtgc tgaagccatt tcgcggtgga tctgactttc ggtagtacga gcgccaatcg
    14161 cgccccaact gattaaatca gcaataaact gaccggttac catatcgagg aactcagtcg
    14221 cggtagggac acccagctca tttacctgta aaagtaattt gcgcgccagc tccagaccgt
    14281 gatttacccg atagctgccg tttaaatccg gatcggagat tagccctttc cagccgacaa
    14341 cagttcgtgg tttttcaaaa taggtgcgca ttacgatttc cagccgtgac tggtactggt
    14401 tgcgcagcga ctgcagacgg gtggcgtact ccattgcagc ggtgagatcg tggatcgagc
    14461 aggggccaat aatgaccaac agtcgcttat cttcaccatt cagtattttt tcaattctgc
    14521 ggcgggagtc ggtgacatgg gtggcgacgc caggcgttac gggataccgt agcgcgagtt
    14581 cggcgggcgt taccaggctc tcaatacgcg cagtacggag ttcgtcagtt ctgttcatta
    14641 caagtctcag aaatttgttg cttctacggc aggactaccg gaaagtgatg cgcatcacga
    14701 taaaccaatc gcagctgatt tcaagtgcga ggtgtgtcac acctcgccgg gtggttagca
    14761 tgataacaaa aaaacaaacc aatgcactag ttctctagta cattcggcga ctaaggccca
    14821 tgatatcgag gatcttggtg gcaatctctt ctaccgaata attggtactg ttaatccacg
    14881 ggatctgatt tttacggtac aaggcttcca cttccgcgac ttccatcctg cactgacgaa
    14941 gcgaggcata gcgactgttc tcccgacgtt cctcgcgaat cgccgccaga cgttccgggt
    15001 cgatagtcag gccgaacaat ttatgctgaa gcggtttgag cgacgcgggt agcaccagat
    15061 tatccatatc gtcggcaata aaggggtagt ttgccgcgcg gataccaaat tgcattgcca
    15121 gatacagact ggtgggggtt ttaccacagc gagaaacacc gagcaggatc acctgagcct
    15181 gatccagatt gcgcaacgaa atgccgtcat cgtgggcgag ggtgtaatca atcgccgcaa
    15241 tgcgcgcatc atatttattg agattattag ggttaaggcc atgggtacga tgagcaatcg
    15301 gcgttggatc cagtttcatc tcttgttgta gcggggcaac cagcgcctga acgatatcct
    15361 ggcaaaagcc ttcactttgc aagatgatgg cgcgaatctc cggcaacacg atggagtaga
    15421 agaccagcgg gcgcacgcct gtctggtgat aaattgcgtc aatctgatcc ttcactgccc
    15481 gtgcacggct ctcattttcg acaaacggca gcgtgatgct gctgatagtg acgggaaatt
    15541 gtgacattac tgcgtgtcct aatacctccg cagttattgc cgtaccatca gaaatataaa
    15601 aaacgtggcg atcaacagca ttatccattt tgttcttccc gtgaagcaga cataattcta
    15661 tgaaagcata aattaaaagc gcacagaagc gtaggacgta atgtctggtt tataaaatga
    15721 accttcattt tattttttat aaaaacagca tttcattttt atggtttcgt ttataccgat
    15781 ggtttatgtg gaaattgtcg aatagagcag gtttgcgcaa cgctgggatc agtcttaaaa
    15841 agtaaaaaaa tatatttgct tgaacgattc accgtttttt tcatccggtt aaatatgcaa
    15901 agataaatgc gcagaaatgt gtttctcaaa ccgttcattt atcacaaaag gattgttcga
    15961 tgtccaacaa tggctcgtca ccgctggtgc tttggtataa ccaactcggc atgaatgatg
    16021 tagacagggt tgggggcaaa aatgcctccc tgggtgaaat gattactaat ctttccggaa
    16081 tgggtgtttc cgttccgaat ggtttcgcca caaccgccga cgcgtttaac cagtttctgg
    16141 accaaagcgg cgtaaaccag cgcatttatg aactgctgga taaaacggat attgacgatg
    16201 ttactcagct tgcgaaagcg ggcgcgcaaa tccgccagtg gattatcgac actcccttcc
    16261 agcctgagct ggaaaacgcc atacgcgaag cctatgcaca gctttccgcc gatgacgaaa
    16321 acgcctcttt tgcggtacgc tcctccgcca ccgcagaaga catgccggac gcttcctttg
    16381 ccggtcagca ggaaaccttc ctcaacgttc agggttttga cgccgttctc gtggcagtga
    16441 aacatgtatt tgcttctctg tttaacgatc gcgccatctc ttatcgtgtg caccagggtt
    16501 acgatcaccg tggcgtggcg ctctccgccg gtgttcagcg gatggtgcgc tccgacctcg
    16561 catcttctgg cgtgatgttc tccattgata ccgaatccgg ctttgaccag gtggtgttta
    16621 tcacttccgc atggggcctt ggggaaatgg tcgtgcaggg tgcggttaac ccggatgagt
    16681 tttacgtgca taaaccgaca ctggcggcga atcgcccggc tatcgtgcgc cgcaccatgg
    16741 ggtcgaaaaa aatccgcatg gtttacgcgc cgacccagga gcacggcaag caggttaaaa
    16801 tcgaagacgt accgcaggaa cagcgtgaca tcttctcgct gaccaacgaa gaagtgcagg
    16861 aactggcaaa acaggccgta caaattgaga aacactacgg tcgcccaatg gatattgagt
    16921 gggcgaaaga tggccacacc ggcaaactgt tcattgtgca ggcgcgtccg gaaaccgtgc
    16981 gctcacgcgg tcaggtcatg gagcgttata cgctgcattc acagggtaag attatcgccg
    17041 aaggccgtgc tatcggtcat cgcatcggcg cgggtccggt gaaagtcatc catgacatca
    17101 gcgaaatgaa ccgcatcgaa cctggcgacg tgctggttac tgacatgacc gacccggact
    17161 gggaaccgat catgaagaaa gcatctgcca tcgtcaccaa ccgtggcggt cgtacctgtc
    17221 acgcggcgat catcgctcgt gaactgggca ttccggcggt agtgggctgt ggagatgcaa
    17281 cagaacggat gaaagacggt gagaacgtca ctgtttcttg tgccgaaggt gataccggtt
    17341 acgtctatgc ggagttgctg gaatttagcg tgaaaagctc cagcgtagaa acgatgccgg
    17401 atctgccgtt gaaagtgatg atgaacgtcg gtaacccgga ccgtgctttc gacttcgcct
    17461 gcctgccgag cgaaggcgtg ggccttgcgc gtctggaatt tatcatcaac cgtatgattg
    17521 gcgtccaccc acgcgcactg cttgagtttg acgatcagga accgcagttg caaaacgaaa
    17581 tccgcgagat gatgaaaggt tttgattctc cgcgtgaatt ttacgttggt cgtctgactg
    17641 aagggatcgc gacgctgggt gccgcgtttt atccgaagcg cgtcattgtc cgtctctctg
    17701 attttaaatc gaacgaatat gccaacctgg tcggtggtga gcgttacgag ccagatgaag
    17761 agaacccgat gctcggcttc cgtggtgcgg gccgctatgt ttccgacagc ttccgcgact
    17821 gcttcgcact ggagtgtgaa gcagtgaaac gtgtgcgcaa cgacatgggg ctgaccaacg
    17881 ttgagatcat gatcccgttc gtgcgtaccg tagatcaggc gaaagcggtg gttgaagaac
    17941 tggcgcgtca ggggctgaaa cgtggcgaga acgggctgaa aatcatcatg atgtgtgaaa
    18001 tcccgtccaa cgccttgctg gccgagcagt tcctcgaata tttcgacggc ttctcaattg
    18061 gctcaaacga tatgacgcag ctggcgctcg gtctggaccg tgactccggc gtggtgtctg
    18121 aattgttcga tgagcgcaac gatgcggtga aagcactgct gtcgatggct atccgtgccg
    18181 cgaagaaaca gggcaaatat gtcgggattt gcggtcaggg tccgtccgac cacgaagact
    18241 ttgccgcatg gttgatggaa gaggggatcg atagcctgtc tctgaacccg gacaccgtgg
    18301 tgcaaacctg gttaagcctg gctgaactga agaaataaaa taaatccccg gcggcgttca
    18361 gtcgccgggg ttatgtgatc cccgaagatg aaacttattc aatctcttca cagacatcct
    18421 gcgttaatcg ccgtataata tcttttctta acaaaaactt ttgtatttta tctgaggcag
    18481 tgcgcggtag tttttcgatt accacgatat gttcaggata tttatatttt gcgacccgtt
    18541 tacggctaaa aaaagccgcc atctcttcca gcgataatga atgatgcggc gctttcagca
    18601 cgacataagc gcatgatcgt tcacctaaac gttcatcggg cattgcaacc acacaggcat
    18661 cgtgaatttt aggatgctgc aataaaatat cttccacttc acggctgcta atattttcgc
    18721 cgccgcggac aataatatct tttttgcgtc cggtaatttt tatatagcca gcctcatcca
    18781 tacggcagag atcgccgctg taataccagc cttcttcatc cagcgcacgg gcggttaatt
    18841 caggttcatc aaaatacccc ataaacacat tggggccacg cgatgcttct tcaccttcgc
    18901 aacctggcgg taaggtcttg cgtgcgtcat cgaccacttt aatctctaca cctgcggcag
    18961 cgtaaccatc ggtatgcata aagcgcgaca aagaattatc gagattcacc accgcatgcg
    19021 gcgaactttc tgtggaacca taaacactta ataatttaat gctgcgctgc tggcattcgc
    19081 gcgccacttt tttggggatt gtggtaccgc cgcaaagaaa gaagcgcagc gctgaaaggt
    19141 ctgcgggttg tttctctagt aaattcaaaa gatcatagac aaacggcgtt gcgcctagca
    19201 tacaggtgca acgctgctgc tcaagcagcg cgagacacgc atcaggagtg aaaatatcta
    19261 acaacacgct gcgagcgcca attaagaatg gtgccgttac gccatgcaga aagcccgttg
    19321 cgtgaccaag tggcgcaggc atcataaaga catccagcca ggtcagattc agccgcgcgc
    19381 aataagcccg ctcactggcg agaatattgt tatgcgttag catcacgccc tttggcagac
    19441 cctcggttcc ggaggtaaac agcaccgcag ctaattcatc gccgtgggtc gttatcgccg
    19501 tggtcagtgg gatattgtcg gcgataatct gacttaatga gagggaagag gtggcgggag
    19561 ccagtttgtc cacgccgaca atttgttgta gttgtggaag ctgattttgc agcggcagga
    19621 ttaaatctac cggacgcgtt tgtttaaaca acgtcggtgc aaagaacatt tttgcctgac
    19681 acttattgag cacccacacc agttctgctt cccgccagga aggcaacagc ggcacggaaa
    19741 ccgcaccgat tttcaggcag gcaagataga taacggtaaa ttcacaccag ccaggcagtt
    19801 gaaatgcgat gcgatcgcct gattcaatac ccttcgctaa catccagttt gccagacagc
    19861 tcgcggcgtg atcgagcgcg ctataggtgt acgatgcacc atgattatcg accacggcaa
    19921 ttttgtctgg catcgcacga gcggtctgct gccagtaatc ggccagtgaa gcatcgcccc
    19981 atagcccttg ctgacgatac gccgcacgac gttgttcgtt aaacgttaat gtcactttca
    20041 ttttggactc tcgaaacaga acatcaggcc cgagttgcgg gcctgtggga tgcatgatta
    20101 gccgtagcga aaatcaatac cgaaggtgcc tgcgggatat tgccactgtt cgagaatagt
    20161 gttaccgcac agcacccgac aggtgccgca ctccagacaa ccggcgggat caaaatgaat
    20221 gtttcctacg tcatcctgct tataaagtcc ggcagggcag gctttcatta atttacggaa
    20281 ttcattgata tcgggatttt ccgtcagaat gatatgcggg tggccctcat caacatggaa
    20341 tttattgacg cctaatttga tgtcgacgtt aaccgtagcg ttctggctca tagcgcggtt
    20401 gctcccttaa tgccatcttt cagcaagttg atcagcccaa ttttcttcgc gtgtcccatg
    20461 atcattttgc gtaccggctg gtttggtttg ccgtcaatgg tgaacatctc gttcatgatg
    20521 tcggctacca ttcgtgggta ttggctaaac aggcgcgggt tttccatcag cgccgggatc
    20581 ttgcgaaaat gctgcatatc acgcatgacg cagctttgtt ccagctcacg tttgtattgc
    20641 gccagactgt tggcggagaa atccgcgcgt tctttggcgg cgatcaccgt tgtggcggca
    20701 gcctgagccg atgcaatggc taaatccatg ccgcggaccg taaaacccaa attcaggcag
    20761 aagcctgcgg cgtcaccaac gatcatcacg ccctcgttaa ccagttgcgg caccattgcc
    20821 agaccgcctt ccggcaccat atgcgcggaa tattcaagca gtttgccgcc gctaatcagc
    20881 gggcgaatgg cggggtgttg tttaaaatct tccagcattt gcggcacgct tttttgcgca
    20941 tgggcgatat cacccaatcc acaaaccagc cccaaggata tggaatcctt gttggtatag
    21001 agaaatcccc cgcccatcag gccgtcagaa ggggaaccgg caaacagcca ggcggcacct
    21061 tcctcgcccg taatattaaa gcgatcgttg atctgttctg gtgtgaggcc aataacctct
    21121 ttaacaccaa cagcgtaatg atgcggatcg gaagcgggaa ccattcccag cgagcggcca
    21181 agcatcgagt taacgccatc agccagaatc accacattcg cttcgagaat atcatcccca
    21241 gcctgcacgc cagtgacctt gtttccttca cgaaccagcg cgtcgacgcg aacgcctggg
    21301 ataaactgcg cgcccgcctg ctcggcttgt tccatcaacc acgggtccag acgattacgc
    21361 aatacggtat aagatgcgtg ttgaggaaca tctggttgct cgcggtgaaa atcgagggta
    21421 acggcgcttt cttcggttaa gaaggagatt ttctcgcgtg tgaccttgcg ttctaccggc
    21481 gctgatactg caaagcctgg aatgattgct tcaagtgtgt gggcataaag acgcccgccg
    21541 gtcatgtttt tacatccggc actgtcgccg cgttctatca ccaggacatc cagcccggct
    21601 cgtgccatga ccagtgcggc aacgctacca gcaacgcccg caccgaccac aatggcatca
    21661 aatttgtcat ccgacatgcg ttatgctccc ttcctgctgc gtttacagcg ggagatgtgg
    21721 ttgaaagggg aattagcggc tcaactggct aatcagggca gggacgactt tgtagatatc
    21781 gcccaccaga ccgtagtcgg catagttgaa gattggcgca tttttatctt tattgatggc
    21841 gacaatcact tttacgccgt tgccgccaac catatgttgg atctgcccgg agatccccag
    21901 cgtcaggtag agatcggatt tcagcaacac gccggagaca ccgatataac gttcacgctc
    21961 catccagttc tcgccttcgg caattggacg tgaacagccg acttcagcat tcagcaccgc
    22021 cgccagttcg tggaccattt tcaggtcatc ctgcgccgcc agaccacgac cgacgccaac
    22081 cacacgtttt gctttgctca ggtccacgct gcttgcggct ttcgcacggc gttcgcgaca
    22141 gagaatttca tgacgcggag caacataagg taccgtttct gtcgggcact gatgagaggt
    22201 atcactggtg cacggttcct gaacaccggg tgcaagggta atgatcgcca gcgggctgtt
    22261 gatcttttcc tgagcgaacg ctaacccgcc atacatccgg tgttcggcgc aaatgtgacc
    22321 atcgacaata tccaccgccg tggcatcgtt caccagcgcc gcattcagtt gcacacttaa
    22381 ccgtgctgcc agagctttac cacgtttcgt cgcggccaac agcaacatag cggggtgttt
    22441 atctttcagc agggcagcaa tgctttcggc gtaattttca gtgcgttgca gcgcgtcgtt
    22501 ttgctcaaga acataaatac attttggacc ataaggcata actgcctgcg cctggtcggt
    22561 attttgtaca atggcataca cctgttggcc ccattgctga gcgccgccaa acagttcagc
    22621 ataacgttca ggattatcgc taaagaccca gacgctgttt aattgactca tcgtttcttc
    22681 tccgtaggct tcagttcagg gctttcttca gatgttccgc cagctcggca atggcctccg
    22741 gcgaatcgtt atcgagaatg atgtgcttac gttctgtttg cggcggtacg cgaatgccga
    22801 ccagttcagc aagtggcgcg ctctggctcc agtcaatatc acttgcctgc cactgattta
    22861 ccggtttttt acccgcgccg agaatggctt tcatcgaagg aatgcgtggc acgttaatat
    22921 cggaggtgac gcagagcacg gctggaacag agagttcaat aacttcaaca tcatcttcaa
    22981 gcgtgcgttc aatcaccagt gtattgccct gacgctgaat agcactcact gcattaatca
    23041 ccggaagttg cagaatttct ccgaccagca agccaacctg ctgggcataa aggtcgccgg
    23101 aaccttcacc aaagatcagt aaatcgaagc cgatcttttc aattgctgcc gccagcgctt
    23161 ttgcggtatc gagaggcagt gcatgttcaa gttgcgcatc ctgcaccaaa tacaggctgt
    23221 gcggcccgcg ggatagcacg tctttgcgca ctttcgagtt ctgcaacaat gagccgccaa
    23281 cggtcagcgc ggctatctca tcgtcatctg ttgcgagctg gcttgcagct tcaatggcat
    23341 tgagatcgaa ctggctgatt ttggcgtcgg cattgtcgaa attcagggtg tattctggag
    23401 tgacaacaat gtcctgttct tcaggcacca gcttaaagca ggttattatt ttcatgattt
    23461 ctcctgtgag tcgcatacgg aaccatctcc gatggaaggt aaaaatggcc tgaaaatagt
    23521 gtgcgatagc ggtgcggggg ggggggcgac ggagcaagct gttttccttg ccaacaatct
    23581 tgcaaaaaaa atgcgattgc taaaacaaaa gtgcaatttg cggcaatata gttattccat
    23641 gaccgtacaa tcttgcattc agtgtaaata atcttgttta gctctcgcta aacgcggttg
    23701 tataaccgca atacacgccg tattttatcc ggcatatatt gtttttgctt gctcatggaa
    23761 tagggaggtt gtcatgtatc aacgctgttt tgataatgcc agtgaaacgc tgtttgtcgc
    23821 cggtaaaacg ccacggcttt cacgttttgc atttagcgat gatccaaaat gggagtctgg
    23881 acatcacgtt catgacaatg aaaccgagct gatttacgtc aagaaagggg ttgcaaggtt
    23941 taccatcgat tcttcgttat atgtcgcaca tgcggatgac attgtggtga tagaacgcgg
    24001 caggctgcat gcggtggcct ctgacgttaa cgatccggca acgacgtgta cctgtgcgct
    24061 gtacggcttt cagtttcagg gggctgagga aaatcagtta ctgcaaccgc attcttgtcc
    24121 ggtaattgcc gcggggcagg gaaaagaagt cataaaaacc ttatttaatg agctaagtgt
    24181 gattttgccg caaagtaaaa atagccaaac atcttcgtta tgggatgcat tagcctatac
    24241 attagc
//
LOCUS       DAZPCS010000044        24184 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZPCS010000044 DAZPCS010000000
VERSION     DAZPCS010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24184
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.044"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(25..597)
                     /locus_tag="ACHUQ7_001787"
     CDS             complement(25..597)
                     /locus_tag="ACHUQ7_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFR1135223.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            815..1675
                     /gene="tesB"
                     /locus_tag="ACHUQ7_001788"
     CDS             815..1675
                     /gene="tesB"
                     /locus_tag="ACHUQ7_001788"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFR1135224.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHCIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(1724..3010)
                     /gene="amtB"
                     /locus_tag="ACHUQ7_001789"
     CDS             complement(1724..3010)
                     /gene="amtB"
                     /locus_tag="ACHUQ7_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFR1135225.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSVGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(3040..3378)
                     /gene="glnK"
                     /locus_tag="ACHUQ7_001790"
     CDS             complement(3040..3378)
                     /gene="glnK"
                     /locus_tag="ACHUQ7_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFR1135226.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(3559..5340)
                     /locus_tag="ACHUQ7_001791"
     CDS             complement(3559..5340)
                     /locus_tag="ACHUQ7_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFR1135227.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGAVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDCIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIIDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(5333..7105)
                     /locus_tag="ACHUQ7_001792"
     CDS             complement(5333..7105)
                     /locus_tag="ACHUQ7_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFR1135228.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLSLMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            complement(7135..7593)
                     /gene="decR"
                     /locus_tag="ACHUQ7_001793"
     CDS             complement(7135..7593)
                     /gene="decR"
                     /locus_tag="ACHUQ7_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HFR1135229.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGVWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(7747..8565)
                     /gene="cof"
                     /locus_tag="ACHUQ7_001794"
     CDS             complement(7747..8565)
                     /gene="cof"
                     /locus_tag="ACHUQ7_001794"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFR1135230.1"
                     /translation="MARLVAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVRTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            8665..10357
                     /locus_tag="ACHUQ7_001795"
                     /pseudo
     CDS             8665..10357
                     /locus_tag="ACHUQ7_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
     gene            10422..11117
                     /gene="queC"
                     /locus_tag="ACHUQ7_001796"
     CDS             10422..11117
                     /gene="queC"
                     /locus_tag="ACHUQ7_001796"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFR1135231.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(11169..11567)
                     /gene="fadM"
                     /locus_tag="ACHUQ7_001797"
     CDS             complement(11169..11567)
                     /gene="fadM"
                     /locus_tag="ACHUQ7_001797"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFR1135232.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGVENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILNQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(11661..12032)
                     /locus_tag="ACHUQ7_001798"
     CDS             complement(11661..12032)
                     /locus_tag="ACHUQ7_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFR1135233.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(12183..14054)
                     /gene="ppiD"
                     /locus_tag="ACHUQ7_001799"
     CDS             complement(12183..14054)
                     /gene="ppiD"
                     /locus_tag="ACHUQ7_001799"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFR1135234.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DVASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGASG
                     INSDIITVDGDRAFVLRVSEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(14246..14536)
                     /gene="hupB"
                     /locus_tag="ACHUQ7_001800"
     CDS             complement(14246..14536)
                     /gene="hupB"
                     /locus_tag="ACHUQ7_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFR1135235.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(14727..17081)
                     /gene="lon"
                     /locus_tag="ACHUQ7_001801"
     CDS             complement(14727..17081)
                     /gene="lon"
                     /locus_tag="ACHUQ7_001801"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFR1135236.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDVPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVIGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(17269..18543)
                     /gene="clpX"
                     /locus_tag="ACHUQ7_001802"
     CDS             complement(17269..18543)
                     /gene="clpX"
                     /locus_tag="ACHUQ7_001802"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFR1135237.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(18669..19292)
                     /gene="clpP"
                     /locus_tag="ACHUQ7_001803"
     CDS             complement(18669..19292)
                     /gene="clpP"
                     /locus_tag="ACHUQ7_001803"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFR1135238.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(19539..20837)
                     /gene="tig"
                     /locus_tag="ACHUQ7_001804"
     CDS             complement(19539..20837)
                     /gene="tig"
                     /locus_tag="ACHUQ7_001804"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFR1135239.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(21181..21498)
                     /gene="bolA"
                     /locus_tag="ACHUQ7_001805"
     CDS             complement(21181..21498)
                     /gene="bolA"
                     /locus_tag="ACHUQ7_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFR1135240.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            21803..22381
                     /locus_tag="ACHUQ7_001806"
     CDS             21803..22381
                     /locus_tag="ACHUQ7_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR1135241.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRALRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            22425..23900
                     /gene="ampG"
                     /locus_tag="ACHUQ7_001807"
     CDS             22425..23900
                     /gene="ampG"
                     /locus_tag="ACHUQ7_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFR1135242.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
BASE COUNT         5667 a         6666 c         5827 g         6024 t
ORIGIN      
        1 ccgggcggag agggttaacg tcgcttaata ttgcgtagga gcgggaactg ccgaagacgg
       61 attcacctga gttggtgaag tcgaaggtac ggtagtcgtt gcgccaccgc tggcctgaac
      121 cggcacggcg gtttgctgta ccggcaccaa tgtcaggtcg gctttagttc cgccctggtt
      181 gatcaccggc tgaacggtat cggtgataaa taccagtttg tcattcacgg taatcgccgc
      241 actcaacaga atacgcgcgt tcggctgaac atctgccggg ttaaatggca gaacaaagct
      301 gaatggtgac tgtttacctt cagtacgcac cgctttctgc gccagcactt ttgacggtgc
      361 atcggctaac gacgcgtcag aaagtgtcac ggtcagcaca gcatcaggcg gcagtgcgac
      421 tttctgacgg atccagacag taccggagac attcggttgc tggatagcag gttgttgtgt
      481 tgctgaaata gacgtatttg cagccggggc tggcgtctga atatccgcgc ttttatctgc
      541 gcaagccgcc aacgcaatcg caaccgctaa accactggcc atgtgcacga gtttcattca
      601 tttctcctta ttatcaatgc accagcgggc taactttcct cgccggaaga gtggttaaca
      661 aaatagtaac gtcaacaagt gtggcacaca tcacgcattt ctgcctgtaa ttagcccgta
      721 attcagacta tttcacccat cggaccagtg gcaaatttgc gttatactca actcactttg
      781 gcttgctgcg gcagctttgt tactggagag ttatatgagt caggcgctaa aaaatttact
      841 gacattgtta aatctggaaa aaattgagga aggactcttt cgcggccaga gtgaagattt
      901 aggtttacgc caggtgtttg gcggccaggt cgtgggtcag gccttgtatg ctgcaaaaga
      961 gaccgtcccg gaagagcggc tggtacattc gtttcacagc tactttcttc gcccaggcga
     1021 tagtaagaaa ccgattattt atgatgtcga aacgctgcgt gacggtaaca gcttcagcgc
     1081 ccgccgggtt gctgctattc aaaacggcaa accgattttt tatatgacgg cctctttcca
     1141 ggcaccagaa gcgggtttcg aacatcaaaa aacaatgccg tccgcgccag cgcctgatgg
     1201 cctgccttcg gaaacgcaaa tcgcccaatc gctggcgcac ctgctgccgc cagtgcttaa
     1261 agataaattc atctgcgatc gtccgctgga agtccgtccg gtggagtttc ataacccact
     1321 gaaaggtcac gtcgcagaac cacatcgtca ggtgtggatc cgcgcaaatg gtagcgtgcc
     1381 ggatgacctg cgcgttcatc agtatctgct cggttacgct tctgatctta acttcctgcc
     1441 ggtagctcta cagccgcact gcatcggttt tctcgaaccg gggattcaga ttgccaccat
     1501 tgaccattcc atgtggttcc atcgcccgtt taatttgaat gaatggctgc tgtatagcgt
     1561 ggagagcacc tcggcgtcca gcgcacgcgg ctttgtgcgc ggtgagtttt atacccaaga
     1621 cggcgtgctg gttgcctcga ccgttcagga aggggtaatg cgtaatcaca attaaaaaaa
     1681 tagccggagg tgaaaaccgt ccggctattt tttgcagtgc ttgttacgcg ttataggcat
     1741 tctcgccgtg gctgttgacg tccagccctt ctcgctcttg ttcttccggc acacgtaggc
     1801 caaccgtcag atccgccagt ttgtagccaa taaatgccac cacaccggac cagacgatcg
     1861 taatggcgat gctttccaac tgtaccagca actgatggcc catcgtcaca ccttcagcga
     1921 agcccacgcc gcccagagaa ctggcggcaa aaatcccggt catgatacag ccgacaatgc
     1981 cacaaacgcc gtgcacaccg aagacatcgc agggatcatc cacccgcagc aagcgtttga
     2041 gcatggtaac gccccacaaa cccgccagac cagctaccac gccgataatc aacgcgccgc
     2101 caaccccaat gtagccgcac gctggcgtca cgccgaccag accggcaatc gcgccagaac
     2161 acgctcccag cagagaaggc ttaccacgca gcgcccattc accgaagatc cagccaagaa
     2221 ttgccgccgc cgttgcgacc acagtattca caaatgccag tgccgcgatt tcattcgccg
     2281 tgcccactga cccggcgtta aagccaaacc aaccgatata gagaatggca gtcccggtga
     2341 agaccatcgg caggttgtgc ggtttaaacg cctctttacc gaagcctacg cgtttgccta
     2401 tcagatacgc gcccaccaga ccggcgattg ctgcgttaat gtgcaccacc gtgccacccg
     2461 cgaaatccag cgcaccgtga gaagccagca aaccaccgcc ccacaccata tgcgcaatcg
     2521 gaatgtaaga gagcgtcagc cataccacca cgaaaatcaa cacagctgag aagcggattc
     2581 gttccgccag cgccccaact atcaagccta cggtaatgca ggcaaacgat ccctgaaacg
     2641 ctacgtggat atactgataa atgctgccca tcaccgccgt cagttcgatg ttttttagca
     2701 tcaaccagtt aatgttgccg aagaagttgt tgccctcacc aaacgccagc gaataaccgt
     2761 aaaccaccca gagaatacag accagtgcaa acgtcaccgt cacctgcgtc agcatcgaca
     2821 gcacgttttt gccgcgaatc aacccaccgt aaaacagggc aatccccgga atagtcataa
     2881 acagcaccag cgcagtacaa atcatcataa acgcattgtc ggctttatcg gccaccgcag
     2941 gtgcagccat taccagtccc ggcagcatcg ccagtgaagc aagcccagtt tttatcgtcg
     3001 ctatcttcat ttttcgttcc tgttgctgtg tgccagagat tacagcgccg cttcgtcggc
     3061 ttcgccggta cgaatacgaa tgacgcgttg caattcagcg acgaagattt tgccgtcgcc
     3121 aatttttccg gtgtaagccg ccttactgac gatatcgatc acttcatcga gttggtcatc
     3181 agcaatcgcc acatcaattt ttacttttgg caggaagttg acgctgtatt ccgccccccg
     3241 gtacagctcg gcatgccctt tctgacgccc gaaacctttc acttcggtga cggtcaggcc
     3301 ctgaatacca atggaagata acgcttcacg aacgtcttcc agcttgaatg gtttgattat
     3361 cacggtcacc agcttcatag atcccctccg gtcagaattc ggtaatggtt ctgctacacg
     3421 aagaaggtat tgcaagcggt gtgccagaaa tgaaaaacga gaaagagagc aggaagttaa
     3481 gaagattgaa aatgaaaacg cactatgaca gtgcacagtg cgttacattt ttgcaccaac
     3541 gatgaaatat tgcgctattc aggcgctcaa tgattcctct tcacgcacgc tggctgccag
     3601 ctcttcgccc gcaagttgca gttgatacat ctgccagtag cgtccctggg ccgccagcag
     3661 ttgctggtga gtgccctgct ccacggcttg cccacgatga agcaccagaa tggtgtcggc
     3721 atcaataata gttgataagc ggtgagcaat caccaccagc gtggtatgtt cacgcaccgc
     3781 cgccagagca tgttgaatcg cctgttcagt accggagtca atgctggcgg ttgcctcatc
     3841 aaggatcagg atttgcggcg tctccaccag cacgcgcgcc agtgccagca gttgcttttg
     3901 cccgactgag agattatttc cctgctcgcc cagcggcgta taaatacaat cactcatgct
     3961 gcgcgccagc tccgccagtt gcacggtttc cagcgcctgc cagacgcgtt cttcggagat
     4021 atcccgcccc agcgtcacat tagcgaggaa ggtatccgcc agcaccaccg gatcttgctg
     4081 caccattgcc acgccctggc gcagcgcgct atgacttagc gaacttaatg gacgaccatc
     4141 aaggtgaatc tcaccttccg ttagcgggta atagcccatc aataaactgg cgagggtact
     4201 tttaccactg ccggtatgcc cgaccagcgc cacaaagtta cgcgaaggca cagagagatt
     4261 aatgttcttt agcaccagat tgtcatcgcg ataagcaaat gacacgttat cgacttcgat
     4321 ggtgccactc tgtaacgggt aatcatcatt gccatattgc tggcgcggtc cgtccatcag
     4381 ttcaaacacg cgctcaccag caacaacagc ctgttgcagc atcgcctgtt gcgtggtcag
     4441 ttcgattaag ggttcgttaa gtcgcccaag atagctgata aacgcataca acacgcccac
     4501 ttcaatggtg ccgctggcgg agaagccaaa cagcatcaat aagccacaaa gaatgagcga
     4561 tgaaaacaga ctcagcagcg gacgcagcag aaaaccgtcg aggcgcaggg tttgcatcct
     4621 agccatatag tgtgaacgac tggcctcccc catacgttcg ccaaatcgcg cctgctgacg
     4681 aaactgctgg ataacgctca tgccattgat gatttcgtta aagccgtcgt tgatatccgc
     4741 cagataggcg cgcacacggc ggacaatcgg cgtgctgtaa cgctggtata tcaccattac
     4801 caccagcacc accgggaaaa tcattatcgc caccagcgcc attcgccagt cgaggctgaa
     4861 catcgccacc agcatcgcgc ccaccagtgc ggcactgcgc aggacagttg ccactacggt
     4921 aacgtagaga tcgcggatca cttcagtgtc attagtgacg cgggaaatca cctgcccgac
     4981 gggttgggta tcaaactcgc ttaatggctg gcgtaacgca gcatccatca cgtcggtacg
     5041 caactgttgc actgcgccta ctgccgcccg attaaacagc agcgactgcg cgtaatgtag
     5101 cccggcggca aacagttgta gcccaacata cgccgcagcc agccctgcaa ccactttcaa
     5161 cggcaggtta tttttcgcta ccatattgtc gataaaatag ctgataagca gcggcccact
     5221 gacttctgcc gccgccgcaa cccacatcat caggaccgca atccccagcg gtttacgcca
     5281 cggcgaacca tacgctaaca ggcgcttgag agtcggccac agttggctaa aactacgcat
     5341 cgacggcctc ctcgcgaatt tccggaacgt cgtcgagcgc cgcctccagt tgttgatagc
     5401 gatacatatc gcgataccaa ccgctttgtt gcgccagctc atcatgattg ccacgctggg
     5461 cgatatgtcc gtgctgcatc acaataattt cactggcttc cgtcagtgcc gaaaggcgat
     5521 gggcactgat gattaccgtt cttccctgtc cccactgacg caggttatgc aggatctggt
     5581 gctctgtgcg tccgtccacc gccgaaagcg catcatcaag gatgaggatt tccgcgttga
     5641 ctaataacgc tcgagcaatg gagatacgct gtttttgccc accggaaagc atcacgccgc
     5701 gctcgcccac ttctgtatcg taaccttgcg gtagacgcaa aatatcgtca tgtacgctgg
     5761 ctaaccgcgc gacatgctca atctcttgct gggtggcatt cgggcaaccc agcgcgatgt
     5821 tattcgccac ggtgtcagaa aaaaggaatg gcgtctggct gaccaccgcc aggcggctac
     5881 gccagctatc gagttgtaac ttcgtcagag gaatatcatg aaagcgaata tccccctcgc
     5941 tgacgtcgaa atgacgctga atgagcgaca acagggtact tttgccggaa ccagtcggcc
     6001 cgcaaatacc cagcatctga ccgggtttca gggcgaaatt gacgttttcc agcgcaggat
     6061 ggtcagtctg cggatacgtg aactggtgaa tatttacatc cagttcgcca cgcccttccg
     6121 gcacgggttc gctaccatcg ttcaccaccg gcgcttccgc cagcatggcg cgaatacggc
     6181 tgtatgcagc actaccacgt tccacaatgt taaacatcca tgccagcgcc agcattggcc
     6241 aaatcatcag acctaaatac atcataaaac tggtgagctg gcccagcgtt aaactgccct
     6301 gcaccaccat ccaactaccg ccgccaatcg ccagcaagtt cgccatacca atcgcgatat
     6361 agatggtcgg gtcgaagcga gcatcaatac gcgccacccg catgtttttt ttgccggtat
     6421 cttcggcatc cgcggcaaat aacgccgact ggcgatcttc cagaccaaag gctttgatca
     6481 tgcggatact ggtgaggctt tcctgggtgc ggtcattaag actggaaaac gccgcctgtg
     6541 ccagcttaaa gcgttcatgc aaggcatcgc cgttgcgctt aatcatgatc gccatcactg
     6601 gcatcggtaa cagggcaaat aaggtcaact gccagctaat ttgcgtagac atcataatca
     6661 acacagcgca gcccattacc agtgaatcca ccagcgtcag caccccttct ccggcggcaa
     6721 acacgacgcg atcgacgtca ttggtcgcac gagccatgag atcacccgtg cgatgacgca
     6781 ggtaaaactc cgggtgctgc cggcttagct gacggtaata atcttcacgc agttcaacag
     6841 ccagttggta agacgcgcca aacagcaata cgcgccagac ataacgcagg agataaacca
     6901 caacggcaat cagcaccatg gtggcgatcc acatcaggct cagcccggta gtaaagtgtt
     6961 gttctgtcac gccatcgaca acaataccaa ccacttttgg cggaaccagt tgcagcatcg
     7021 cgataatgac aagcaaggcg acagccccaa gatagcgacg ccattcccga cggaaatacc
     7081 agcttaattg agcaaataat cgcacgcgtt gtgtcctgac ctgattctgg atatttattc
     7141 gatgggtaaa gaagtggtgt atttaatctg ttccatcgcg aagctggaag tgacgtccga
     7201 cagccccggc acgctgttta ccagacgctt ataaaactcg tcgtagcgtt tcatgtcggc
     7261 aacctggacg cgcatcagat aatcgtattc accagccatg cgccagaccc ccagcacttc
     7321 tggcatttcg gtaaccaccg tgacaaagcg gcaataccat tcgctgctgt gatgttgcgt
     7381 ttttatcagc acaaaggcgg tcaggccgag gcctattttt tccggatcca gcagggcgac
     7441 tttgccgata aggataccgt cgtcctccag ccgtttcagg cgcttccagc agggggtggt
     7501 tgtcagatta acggcttcag ccagtgcctg caaagagagg gtgcaatcct gctgcagtaa
     7561 ggccagcagc ttacggtcaa ttttatctaa catagccctt ccacggagaa tttttttctc
     7621 ttttaattct attttaaagg tgaaatcgcg acaatttatt ctgtgcggtt taaacaccac
     7681 ggcacaaaat gacaaattca acgcaaaaaa aaaccgggca attgcccggt gctggaaggg
     7741 atctcgttat tcgggggaat aaggtagatg tggatagtcc agccagtgcg tcaaatagtg
     7801 agagacagcc tgatttcggc aatgtccaat caccggtaaa tgcgggagct ccgcgcgcag
     7861 ttgcggcatc gcattgccca taataaaccc gctaccgacg ctgcctaaca tttcgcgatc
     7921 gttcatcgca tcaccaaagg ccatgcaatc gcgcaacgat aaacctaaat gttgggtccg
     7981 caccgtcaat gcagcgcctt tattgcagcc caccggcagc acttcgaggc aatccgtggc
     8041 ggaaaaacac aaatgtgcac gctcgcctaa tgcttcgtat agctggatct gcaagcgtgt
     8101 aagatcgtcg tgatcgccac agaagcagat cttggtgacg ctgccgagtg gcattttttt
     8161 gacatcgatt atctgataac gaaaaccgct atagacaaat acctgcaaca acgcagggct
     8221 ctctttcccg gtaaaccaac cgtcgtcatt gaagatatgc atgctggctc gggtatccca
     8281 ttgctgatac agcaccagct ccgcgacatc cgcaggtaaa tcatcacgat gtaaaagttc
     8341 accttccaga gaatgcacgc gcgttccgtt gccggtaatc aaatatgcat ccagcgatag
     8401 cgccccgaga atatgctgca tctccagcgc atgacgcccc gtggcaaaag tgagggtaat
     8461 gtcgcgttca cgcagtcgcg ccaaagtaga gagggttttc tcacctaaat gatggtcagg
     8521 catcaataaa gtgccatcca tatcaaatgc taccagacga gccatttctt tctccactct
     8581 gtgacacggt taatggtgat tgagtatcac ctgatatata cggaagtaat agtgaatagt
     8641 taaataatat tgttccgggt ttatatgcga ttgctcaacc gtcttaacca gtatcaacgt
     8701 ctgtggcaac cttcagccgc aaaccgtcac cgtcagcgaa ctggccgaac gctgtttttg
     8761 cagcgaacgc catgttcgta cgctgttgcg tcaggcacag gaggcgggat ggctggagtg
     8821 gcaggcgcag tcaggacgcg gaaagcgcgg acaattacgc tttctggtca cgccggaatc
     8881 gctacgcaat gcgatgatgg aacaggcact ggaaaccgga aagcagcaag atgtgctgga
     8941 gctggcgcaa ctggccccag gtgagctgcg cactctgtta cagccattta tgggcggaca
     9001 atggcaaaac gatacaccca cgttgcgtat tccctactat cgcccgctcg aaccgctaca
     9061 accaggcttt ttgcccggcc gtgccgagca gcatctcgcc gggcagatat tttccggcct
     9121 gacccgcttc gataataata ctcagcgccc gattggcgat ttagcgcatc actgggaaac
     9181 ctctactgac aggttacgct gggactttta tcttcgttca accctacact ggcataacgg
     9241 cgatgcagta aaagcctcac acttacacca gcgattattg atgctgttac aactgccagc
     9301 actggatcaa ttatttatta gcgtgaagcg tattgaagtc acccatccgc agtgtctgac
     9361 cttcttttta catcgccccg attactggct tgcgcaccgg ctggcgagct attgcagcca
     9421 tctggcgcat ccgcaattcc cactgatcgg cactggtcct tttcgcttaa cacaattcac
     9481 agcggagctg gtgcgcctgg agagccatga ttattaccat ttgcgtcatc cgctgcttaa
     9541 agcggttgag tactggataa ctccgccgct tttcgaaaaa gatttgggaa ccagttgtcg
     9601 gcatcccgtg caaatcacca tcggcaaacc ggaggagctg caacgggtca gccaagtcag
     9661 tagtggcatc agtttaggtt tttgctattt aacgttgcgc aaaagtcccc gactctccct
     9721 ctggcaggcg cgaaaagtga tctccattat tcatcaatcc ggtttattac aaacgttaga
     9781 agtcggagaa aacctgatca ccgccagtca tgcattactg ccaggctgga ctattccgca
     9841 ctggcaagta ccggatgaag tcaaactacc gaaaaccttg acgctggttt atcacctacc
     9901 gatagaactt cataccatgg cagaacgcct acaggcgaca ctggcagcgg aaggctgtga
     9961 actcacaatt atttttcata acgcaaaaaa ctgggacgac acgaccctac tggcacacgc
    10021 agacctcatg atgggcgaca gattaattgg cgaagcaccg gaatatactc tggagcaatg
    10081 gctacgttgc gatccactat ggccacatgt tttcgacgct ccagcatatg cacatctgca
    10141 atcgacgctg gacgcggtgc aagtaatgcc tgatgaggaa aaccgattta atgccctgaa
    10201 agcggttttt agccagttaa tggcagacgc gacgctgacg ccgctgttca actatcacta
    10261 tcgcattagt gcccctcccg gcgtgaacgg tgtgcgactg acaccgcgcg gctggtttga
    10321 atttaccgaa gcctggcttc ccgcgccgtc gcaatgaata gctggtccgg gttaatcgca
    10381 ggcgttacca taacgctttt attgttcaag caggattatc tatgaaacgt gctgtcgttg
    10441 tgttcagtgg aggccaggat tccaccacct gtctggtgca ggcattacaa caatatgatg
    10501 aagtccattg cgtgacgttc gattacggtc agcgacatcg cgcagaaatc gacgtggcac
    10561 gcgaactggc gctgaaactg ggagcacgcg cgcataaggt tcttgatgta acgctgctca
    10621 acgagctggc ggtcagcagc ctgacacgtg acagcattcc ggtgcctgat tatgaacctg
    10681 aagccgatgg catcccgaat acgtttgtcc cagggcgtaa tattttgttc ctgacgctgg
    10741 cggcaatata tgcgtatcag gtaaaagcag aagccgtaat taccggcgtc tgtgaaacgg
    10801 atttctccgg ctacccggat tgccgcgatg agtttgtgaa agcactaaac catgccgtca
    10861 gtttgggcat ggcgaaagat attcgttttg aaacgccgct gatgtggatt gataaagcgg
    10921 aaacctgggc gctggcagat tattacggca aactggattt agtccgtaac gaaacgttga
    10981 cctgctataa cggcattaaa ggcgacggtt gcggtcattg tgcggcatgt aatttacgcg
    11041 ccaacggttt gaatcattat ctggccgata aaccgacggt gatggcagcg atgaagcaga
    11101 aaaccgggtt gaggtaatta ttccgggtgt cgccggatgc ggcttgagca tccggcacca
    11161 caaaacgttt acttaaccat ctgctccagc ttttcgcgca attccccttc cagagctaat
    11221 gctttctgcg ttttaagatc aatacaaaca aacgtaataa gcgcatccgc taccacctgc
    11281 ccttccggct ccagtgtaat gacctggttt aagatgccgc ttttaccgtt taattgctgc
    11341 aactgactgg taatagttaa caggtcgctt aataccgctg gacgacgata gttaatattg
    11401 atattgacca cgacgaaggc gatgttatgg gccgtcatcc actgaaaact gtcgctattt
    11461 tccaccccat cccagcgggc ttcttcgaga aattcaaggt agcgggcgtt gttgacgtgc
    11521 tggtaaacgt cgagatgata tccacgaact ttgatttgtg tttgcatagc gcaataacct
    11581 tacgttgttg ttataagtac agaggtcata actggtatga cctcttcagt ctggcaaaaa
    11641 attgccacta tgcaaattaa ttacagggtt aataccgcca gattacgttc caccagcgaa
    11701 ttgcccatcc ccggcacctg ctttagatcc tccaccgttt taaacggacc gtactcttcg
    11761 cgataactga caatcgcctg cgctttcttc aggccaacgc cattcatcgc gcgggctagc
    11821 tcttccgcgc tggcattatt aatgctgacc cgggtgcctt cttcgtcact ggctttcgcc
    11881 ggtactgctg ctttactttg tgctgcagga gcttccgctt tggtttctac cgccgtcggt
    11941 ttcgccacag aagctgccgc cagcgcgcta tgagacattc cggcacaggc cagggacagt
    12001 gtaatgagca gtgctttaat tccgtgtttc atactgtttt ctccttgttt gttaacagtg
    12061 gaatcacctt aacgggagtg aaaacagtaa acaaatggca atgttcagaa atggaaaagg
    12121 ccgcgaaagc gaccttttct tgttacagct gcaacgcatt tttttgcgag gcggcttcag
    12181 gattattgct gttccagcgc atcgccaatt ttgattttcg cctctttacg caggttactc
    12241 atcagagctt caaagacgat ttgtgcgttg ttctgggtga taccctgcac catcgctttt
    12301 ttctgatctt ccggcattga accttgtttc acttcatcca gcgccagcag aaccacatta
    12361 ccttgcatat cggtcgccat accgtagctc ggtttgtctt tcgctggcag tggcagtgca
    12421 aacgccgcct ggctaatcgg gtcacgaccg gaacggctta aggttttcgg ctcgccaaat
    12481 ttcagaccgg cagcctgcat agcttccgca cctttgccgg ctttcaaatc aaccagcagt
    12541 ttctcagcat ccactttcgc ctgttgttca gctttgttgt gctgaaccag tgccttaact
    12601 tgttcctgaa catctgccaa cggtttcacc gcttccggtt tgtgctcgct gacgcgcagc
    12661 acgaatgcgc ggtcgccgtc tacggtgatg atgtcagagt tgatgcccga cgcgccgttt
    12721 tcacctacca gaccgccgtt aaagatagcg tcggcaaccg gcttgaagtt caactcttcc
    12781 ggcaggttat ctttgctgaa ccaacccgtc tgagtggctt taacgccggc agcttgctct
    12841 gcaccggcca gagactcggt gtcgttgctt gccacatcgc tcactttctg ctgcagcgca
    12901 tagtacgcat cgagggcttt ttcgtgtttc actttcgccg caatgtcgtc acgtacttcg
    12961 tctaacgatt tcactttcgc tggctgaatg tcgtccagac gtacaatcag gaaaccgacc
    13021 gaagatttga tgacaccaga cagttggcct ttttctttca gaccagcatt tttcagttcg
    13081 tcggggatag tggcatcttc taaccaaccc atatcgccgc cgttacgggc agagataata
    13141 tcggcagatt tttctttggc taatgcagca aaatcaccgc ctttattcag ctcatcaagt
    13201 accgctttcg cttcatcttc agttttggtc tggatgatgc tgtagcgggt acgctgcggc
    13261 tgggtgaatt gatcctgatg ctgatcgtag tagctctgga tatccgcatc gctaaccggt
    13321 tgctgcatcg ttgcggcaac cagcttgatg taactcacgc ggaattgttc cggcgtcatg
    13381 aaattgtttt tgttttgttc gtagtagctg gcaatctcct gttcggtcac aggctgcttc
    13441 gccgccagcg cgttaacatc gatagtcgcc tcacgcacca cgcgttgttg cgcgaccagt
    13501 gccgccagct cgtcggtttc acctttcagc ataaaatcgg taccggcaac gccgttaatc
    13561 agctgttggg tagtgagctg gttacgcagc gcctgggcgt actgatcggc ggtcatcccc
    13621 atctggttga ggataccgtt atagcggctg ttatcaaatt tgccgtcaac ctggaaggct
    13681 ggggtcgcga aaatcgcctg tttaacctgc tcatcactga tacccagttt cagctcacga
    13741 gcgtactgat ccaacagcgc ctcgtcgatc agacgattca gcacctgttg acgcagggtt
    13801 ttcatatagc cttcgttcgc tgccagctcg gagtattgat cgcccagctg ttgctgcata
    13861 cgattacgct cgctgttaaa ggcgttctcg aactgcccac ggctgatttc ctggtcattc
    13921 acttttgcgg cgtagttatt gcctccgcca atcaggtaac cactcacgcc ggtcaatatg
    13981 aacgacacga taatgatacc gaaaataatc ttgagcacga gactgtttgc agccgtgcgt
    14041 aagctgtcca tcatggtgta acaacactcc gctgtagatg actgtgtacc tcacgcaacg
    14101 tatcgatgcg cgcaaaggcc tattgtgaca agaaacgggg gcaattgtca gcccacaaga
    14161 cacagaaagt caccgaatac aaataaaaaa ggcacatcag tagatgcgcc cttgaacttc
    14221 gtcacatccc cactggggac aacgcttagt ttaccgcgtc tttcagtgct ttacctgcac
    14281 ggaagctcgg tactttagcg gcagcgatgg tgatctcttt accggtctgc gggttgcggc
    14341 cagtacgggc agcacgctct ttaacggcaa aagtaccaaa acctaccagt gctacatcat
    14401 ccccttcttt cagagattca gttacggaag caataatagc atctaacgca cggccagccg
    14461 cagctttaga gatatcagcc cctgcagcaa tcttgtcgat caattgagat ttattcactc
    14521 ttctcttcct ctttataatt tatatcgcac ttgaatcctt caaggtacga acgcgcagca
    14581 gttatatcag gccagccatc cccttacaac accccttaat aagggcaagc ccgatccgct
    14641 atctaactta gcgagacaaa aaaaggctgg caagcccgaa ttagcctgcc agccctgttt
    14701 ttattagtgc attttgcgcg aggtcactat tttgcagtca caacctgcat gccagacggt
    14761 tcattttgca gcgccagagt cagaacttcc tcaatgcgct tcacaggatg aatgtccaga
    14821 tcggcaatta cgttgtcagg aatctcttcc agatcgcgtt tattttcgaa cggaattagc
    14881 actgttttaa tcccgccgcg atgcgctgcc aggagttttt ctttcaaacc accgatcggc
    14941 agtacctgac cacgcagagt gatctcaccg gtcattgcca catcggcacg aaccgggtta
    15001 ccggtcaggc aagaaaccag cgcggtgcac atagcaatac cggcactcgg accatctttc
    15061 ggcgtcgcac cttccggtac gtggacgtgg atgtcacgtt tttcgtaaaa atcagggttg
    15121 atccccagtt tttccgcacg cgcacgaacc accgttaacg ccgcctgaat agactcctgc
    15181 atcacttcgc cgagcgaacc ggtataggtc agtttgcctt tacccggaac acatgcggtt
    15241 tcaatggtca gcaagtcacc gcccacttcc gtccacgcca gaccgattac ctgaccgaca
    15301 cggttttcgt tatcagcgcg accatagtcg aaacgctgaa caccgaggta gtcatgcagg
    15361 ttatcgccgt taatttcgat atgttttaat gacttatcga gcagtaactg cttaaccgct
    15421 ttgcggcaca gtttggagat ttcacgctcc agaccacgca cgcccgcctc acgggtgtag
    15481 taacgaataa tgccgataat ggcgctatcg tcgacggtca gctcaccttt tttcagtgca
    15541 ttacgttcaa tctgcttcgg cagcaggtga cgtttggcga tgttcagttt ttcatcttcg
    15601 gtataaccgg agaggcgaat cacttccata cgatccagca gcggtgccgg aatgttcatg
    15661 gagttcgacg tcgcgacaaa catcacgtcg ctgagatcgt aatccacttc caggtagtgg
    15721 tcgctgaacg ctacgttctg ctctggatcc agcacttcaa gcagtgcaga ggccggatcg
    15781 cctcgcatgt cagaagacat tttgtcgatc tcatcgagca ggaacagcgg atttttcacg
    15841 cccactttcg ccattttctg gatcagttta cccggcatag aaccgatgta agtacggcgg
    15901 tgaccacgga tttccgcttc atcacgcacg ccgcccagcg ccatacggac atatttacgc
    15961 ccggtggctt tggcaatgga ctgaccaaga gaggttttac ctacccccgg cggccctacc
    16021 aggcagagga tcggtccctt gattttgttg acacggcttt gaaccgcaag gtactcaagg
    16081 atgcgatctt tcacgcgctc cagaccataa tggtcggtat caaggatttc ctgcgcctga
    16141 cgcaggtctt ttttgacctt gctgcgcgca ttccacggta cctgtaccat ccagtcgata
    16201 taaccacgca ctacggtcgc ttctgccgac atcggagaca tcattttcag cttctgcaac
    16261 tctgcttccg ctttctcttt tgcctctttc ggcatcttcg ccgcgtcgat tttgcgcttc
    16321 agggcttcgt tttcgtccgg cacgtcgtcc atttcaccga gttctttctg aatagctttc
    16381 atttgctcgt tcagatagta ctcacgctgg gatttctcca tctgcttttt aacgcggttg
    16441 cgaatgcgtt tctcaacctg cagcagatcg atttccgatt ccatcattgc catcagatat
    16501 tccagacgtt cgttaacgtc ggacatctcc agaacggact gtttgtcagc cagtttcagc
    16561 ggcatatgtg cagcaatggt atccgccaga cgcgccggat cgtcgatgct attcagcgac
    16621 gtcagcactt ctggtgggat ttttttgttc agcttgatgt agccttcgaa ctggctgatt
    16681 gcagtacgca ccagcacttc ctgttcccgc tcatcaatgg tcggcgactc cagatactcc
    16741 gccttcgcag aaaagtgttc gccattgtca gagagcgcag aaatacgcgc gcgctgtaac
    16801 ccctcgacca gcactttgac ggtgccgtca ggcagtttca gcatctgcaa tatagaggcc
    16861 acggtcccga cggtgaaaag atcgtttaca cccggctcat ccgttgaagc ttctttctgc
    16921 gcgaccagca taattttttt atcatggtcc atcgccgctt ccagacaacg gatagatttt
    16981 tcccgcccga caaataaggg gatgaccatg tgcggataaa ccaccacatc gcgcagcggc
    17041 aatacgggga tttcaatgcg ttcagaacgc tcaggattca tagagctctc tcttagttta
    17101 atttccgcca ggtaatcaga tgacacgact gtgcttcacg ccatctatta acatgtacgt
    17161 cagtatatgg ggatgtttcc cccacattca acgccgagaa tagaggaaaa attaaagggg
    17221 agataaaatc cccccttttt ggttaactaa ttgtatggga atggttaatt attcaccaga
    17281 tgcctgttgc gcttccggct taccataaat cagcaacggt ttgctttgac catcaattac
    17341 cgactcgtcg ataaccactt tttcgacgtc ttccatggac ggcagatcgt acatggtatc
    17401 gagcagtgcg gcttctacga tggaacgcag gccacgggca ccggttttac gcgccatcgc
    17461 tttcttagcg atagcatcca gcgcctcgtc acggaattcc agatccacgc cttccagatt
    17521 aaacagcgcc tgatactgct tggtcagggc gtttttcggc tctttgagga tctgaatcag
    17581 agcttcttcg ctcagttcat tcaacgttgc tacaaccggc agacgaccaa tgaactcagg
    17641 gataagacca aacttgatca gatcttccgg ttcaacctgc gccagcagct cgccttcgct
    17701 tgctttgtcg gacttcgctt ttaccgtcgc gccaaaacca atgccggagc cggtttctac
    17761 acggtgggaa atcactttat ccagaccggc aaacgcaccg ccacaaataa acaggatctt
    17821 agaggtatca acctgcaaga attcctgctg cggatgttta cgcccacctt gcggtggaac
    17881 agcagctacc gtaccttcga tcagtttcaa cagtgcctgc tgtactcctt caccggaaac
    17941 gtctcgggta atggacgggt tgtctgactt acgagaaatc ttgtcgattt catcgatgta
    18001 gacaatcccg cgctgcgctt tctgtacgtc gtaatcgcac ttttgcaaca gcttctgaat
    18061 gatgttttca acgtcttcgc ccacataacc ggcttcggtc agcgtggttg cgtcggccat
    18121 ggtgaacggg acgtccagca ggcgcgccag cgtttcagcc agcagcgttt taccggaacc
    18181 ggtcggaccg atcagcagaa tgttactttt gcccaactcg acaccattgc tggtatcgcc
    18241 gttgcgcaga cgtttgtaat ggttgtatac cgcgaccgcc agcacttttt tcgcctgttc
    18301 ctggccgata acgtaatcgt ccaggtggtt gcgaatttca tgcggcgtcg gtagcgcact
    18361 gcgttcacga tgcggtgcaa cttctttaat ctcttcgcga atgatgtcgt tacataaatc
    18421 aacacattcg tcgcagatat acacggatgg accggcaatc agcttgcgca cttcatgctg
    18481 gcttttgccg caaaaagagc aatacagcaa tttgcctgag ccatctttgc gtttatctgt
    18541 catgggtcaa aacctcttct ttgttctttg tgccgcacac gacgacgcaa atgccattct
    18601 caggcgcaag ccgcttacag cgttgtgccg ccctagataa gtatagcggc acagttgcgc
    18661 ctctggcatc aattacgatg ggtcagaatc gaatcgacca gaccgtattc caccgcttca
    18721 ggggcggaaa ggaagcgatc gcgctcggta tcacgttcaa tctgttctaa tgattgaccc
    18781 gtatgaagcg ccataagttc attcatgcgc cctttaactt tcagaatttc acgggcatga
    18841 atttcgatat cggtcgcctg gccctggtag ccgcccaacg gttggtgaat catcacgcgc
    18901 gaattcggca ggcaaaaacg tttacctttt gcccctgcgg tcaacaagaa agcgcccatc
    18961 gaggccgcct ggcccataca gatggtgctg acatcaggct tgataaactg catggtgtca
    19021 tagatagaca tcccggcagt gatcaccccg cctggggagt taatgtacag atagatatct
    19081 ttttctgggt tttccgcttc caggaacagc atctgcgcca caatcaggtt agccatgtgg
    19141 tcttcaacct ggccagtcag aaaaatgacg cgttccttaa gtagacgaga atagatatca
    19201 aaagagcgct caccgcgtga ggtctgttca atgaccatcg gcaccagcgc catatggggt
    19261 gcaaagttat ctcgttcgcc gctgtatgac atttccgtct cctggataaa attgaaaaaa
    19321 acctgctgta ccgattgtaa ccttatggac ggattatcag ctagtccctg tgttatgggt
    19381 acggcatcac catatttcaa gcataacaat cttttgttgt tacgctaaca ccgaaagagg
    19441 cttttcgcac ggctccatgc aaaattcatg acaaaaaaaa gcccaccacc tggcggtgac
    19501 gggcctttgt gcgaatttag cgcgttatgc tgcgtaaatt acgcctgctg gttcatcagc
    19561 tcgttgaaag tggtttcttt ttcagtcact ttcgctttcg ccagtacagc ttcaacagcc
    19621 tgttcttcca gagcaacatt gcgcatgttg tccatcagtt ctttgttttt gctgtagaac
    19681 tcgataactt ctttcggatc ttcgtacgca gaagccatct cttcgatcag gcctttcacg
    19741 cgctcttcgt cagctttcag ctcgttggtg cggataactt cgcccagcag caggccaaca
    19801 actacgcggc gtttagcctg ttcttcgaac agttcgcgcg gcagttccag agcttgtttt
    19861 tcgttgccac cgaaacgctg tgcagcctgg cgacgcagaa cgtcgatttc gctgtcgatc
    19921 agcgcagccg gtacgtcgat gtcgttagct tttaccagac cttcgatcgc ctgagactta
    19981 acgcggttac ggatggcgct cttcagctcg cgctccatgt ttttacgcac ttcagcgcgc
    20041 agaccttcta cggaaccatc ttcaacgccg aaacgtttga tgaattctgc agtcagttcc
    20101 ggcagttcac gctcttcaac tttcttcagg ttgatagcga atttcgctgc tttacctttc
    20161 aggttttctg cgtggtattc ttccgggaag gtcacgtcga tggtgaactc ttcgccagct
    20221 ttgtggcctt tgataccgtc ttcaaagccc gggatcatac gaccctggcc catcgccagt
    20281 acgaaatcag acgctttacc gccttcgaac tcttcgccgt ctacagaacc ggtgaagtcg
    20341 atggttacgc ggtcttctgc ttcaacagcg ccgtcttttt ctttccaggt cgcctgctgt
    20401 ttacgcagag tatccagcat gccgtcaacg tcagcgtcgg tcacttcaac gatcggtttt
    20461 tcaacttcga tcgcttccag accctgcagt tcaacttccg gataaacttc aaactctaca
    20521 gagtaagtga agtcttcacc cagcttgtat tcgcccggaa cataagtcgg tgcgccagcc
    20581 ggattgattt tttctttaat gatggcgtca atgaagttac ggctcatcag gtcacccaga
    20641 acgtcctggc gtacagacgc gccataacgc tgagcaacga tattcattgg cactttgcct
    20701 ttgcggaagc cgtcaatacg tacttttttc gcaacgttga ccagctcgct tttaacagcg
    20761 gtctcgatgc tgtcagcagc gatagtaatc gttacacggc ggccaaggcc ttgagtggtt
    20821 tcaactgaaa cttgcatctt gttacctcaa aaaatcacag tgctcggtca actcttaccc
    20881 cgcaagcaca ggttgttggc ttcgcggaac cgggatgttc ttataatcaa tcacattccc
    20941 tgttaccaga atcatcccga aagcattcaa ataggacgcg gcattatagc ggcatcactt
    21001 ttatgagtcg agaacggtta ttgcgcgctg tcgcgtattt tttcacaatt cccgggcaat
    21061 tttcgtctga atgctgggca aaaagcgcaa aatccagaca aaacaaaacg gcccgcaggc
    21121 cgtttttcat aaaatcttta gcaacatact ggaaaagcgc cgacagttgc aaatgcgttt
    21181 ttacgcgatg cttcctgctc cacgacaggg aggagaggca aagacggtgt cctgcaaccc
    21241 ttcccactcc ttaatagtgt aagtatgcag agccagcgca tgaacggtag tagagagttc
    21301 ctccgctaaa gtactgtaaa tcattcgatg acgattcaga aaacgttcac ccgtaaaacg
    21361 atcgctgacc agcacaactt taaaatggct ttcagagccg gctgggacat tgtgacgata
    21421 gctttcatcc actacttcga ggaatacggg ttggaacgcc gcccttaatt tttcttctat
    21481 ccgctcacgt atcatcatga aatatctcct ccgacaacgc tgagatgtca tccatccctt
    21541 taaatactag ccgcttttac cgtttccatt gcagcttaac aacaaatatt taggtttcgt
    21601 cctggttttt ccgtcaagcg tatgaagttt actgatttaa agattgaaac acaggcctcg
    21661 catcagcgtt tcttttacca ggggtgatta tttcgtcaac atgatgaatt tacatgcctt
    21721 acccacttcc cctcgccgtt ggcaatgtta tgatggcggg aatttttacc aaacacgctt
    21781 aagagtctct gagagcccga acatgttcaa aaaaatcctc ttcccgttag ttgctctgtt
    21841 tatgcttgca ggatgcgcaa aaccgccaac aactattgaa gtttccccga cgattacact
    21901 gccacagcag gatccaagcc tgatgggcgt caccgtaagc attaatggtg ccgatcagcg
    21961 taccgatcag gcgctggcaa aagtcacccg cgataatcaa atcgttaccc tgaccgcctc
    22021 ccgcgctctg cgtttcctgc tgcaagaagt gctggaaaaa cagatgaccg cgcgtggtta
    22081 catggttggt ccgaatggcc cggttaatct gcaaatcatc gttagccaac tgtatgctga
    22141 cgtgtcccag ggcaacgtgc gctacaacat cgctaccaaa gcggatatcg ccatcatcgc
    22201 taccgcgcag aatggcaaca aaatgaccaa aaactatcgt gccagctaca acgttgaagg
    22261 tgcgttccag gcctccaaca aaaatatcgc cgatgcggtt aacagtgtgc tgaccgatac
    22321 catcgctgat atgtctcagg acaccagcat tcacgaattc atcaagcaga acgcgcgtta
    22381 atttctgccc tctggcccgg tgcaagccgg gcctgtagac gcccatgtcc agtcaatatt
    22441 tacgtatttt tcaacagccg cgttcagcca tattgctgat cctgggtttt gcttccgggc
    22501 taccgctcgc cctgacatcc ggcaccttac aggcctggat gacggtcgaa aatatcgatc
    22561 tcaaaaccat tggtttcttc tctctggtag gccaggctta cgtttttaaa ttcctctggt
    22621 caccgctgat ggaccgctac acgcccccat tttttgggcg gcggcgcggt tggctgctcg
    22681 ccacgcaaat cctgttatta gtcgccattg cggcgatggg ttttctcgaa ccaggcaccc
    22741 aactccgctg gatggcggcg ctggcggtgg tgatcgcttt ttgctctgcc tcccaggata
    22801 ttgtcttcga tgcgtggaaa accgatgtgc tcccggcaga agaacgtggt gcgggcgcgg
    22861 caatcagcgt gctgggttac cgtttaggga tgctggtttc cggcggccta gccctgtggc
    22921 tggcagataa atggttgggc tggcagggca tgtactggtt gatggcggcg ctgttgatcc
    22981 cctgtattat cgcgacgttg cttgcaccag aaccaaccga caccattcct gtgcccaaaa
    23041 cgctggaaca agcggttgtt gcacctctgc gagatttctt tggtcgcaat aatgcctggc
    23101 ttattttgct tcttatcgtg ctgtataagc tgggcgacgc attcgccatg agcctgacaa
    23161 ccacgttttt gattcgcggc gtcgggtttg atgcgggtga agtaggcgtg gtaaacaaaa
    23221 cgcttggctt actggcgacc attgttggcg cattgtacgg tgggattttg atgcagcgcc
    23281 tgtcgctgtt ccgggcgctg ctgattttcg gcattttaca aggtgcgtct aacgctggtt
    23341 actggctgct gtcgattact gataagcatc tctacagcat gggcgcagcc gtctttttcg
    23401 aaaacctctg tggcgggatg ggcacatcag cctttgtcgc gctgttaatg acgctgtgta
    23461 ataagtcatt ttccgctact caatttgccc tgctctcagc gctttccgct gtagggcgag
    23521 tttatgtcgg ccccgtggcg ggttggtttg ttgaagcaca cggctggtcg acattctatc
    23581 tattctccgt cgccgctgcc gtaccggggc ttattttgct gctggtttgc cgccagacgc
    23641 ttgaatatac acgagtaaat gacaacttta tctcccgtac cgaatatccg gcaggttatg
    23701 cctttgccat gtggacactg gcggcgggcg tcagcctgtt ggccgtgtgg ttacttctgt
    23761 tgacgatgga cgcgctggat ttgacgcact tctctttcct gcctgctctg ctggaagtcg
    23821 gggttttagt cgccctttct ggcgtcgtgc ttggtggttt gctggattat ctggcgctac
    23881 gaaaaacgca tctgacgtaa tctgtaaata ttatttagct gtagtaatca ctcgccgtaa
    23941 ttattacggc taaataaaca tcagcatcgc ttattaatat ttgttctttt gtgcggctta
    24001 gcgtttggaa attattgacg ccatttataa attctatttt tcttacacga ttcagctaat
    24061 gagtctttat tttctcacca cccagttgtc actttaatga taattatttg ttaaataatt
    24121 gttttatttc gcattggtta taccaattgc ccaccaagac tccttctgca ctcccctttt
    24181 gtta
//
LOCUS       DAZPCS010000045        23323 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZPCS010000045 DAZPCS010000000
VERSION     DAZPCS010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23323
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.045"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..246
                     /locus_tag="ACHUQ7_001808"
     CDS             <1..246
                     /locus_tag="ACHUQ7_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFR1135243.1"
                     /translation="VTIPRGQLKNLKASYTLTEPQLTAPLKKGQVVGTIDFQLNGKSI
                     EQRPLIVMENVEEGGFFGRMWDFVMMKFHQWFGSWFS"
     gene            complement(293..1051)
                     /gene="deoR"
                     /locus_tag="ACHUQ7_001809"
     CDS             complement(293..1051)
                     /gene="deoR"
                     /locus_tag="ACHUQ7_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFR1135244.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1109..1705)
                     /gene="ybjG"
                     /locus_tag="ACHUQ7_001810"
     CDS             complement(1109..1705)
                     /gene="ybjG"
                     /locus_tag="ACHUQ7_001810"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFR1135245.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            1990..3216
                     /gene="mdfA"
                     /locus_tag="ACHUQ7_001811"
     CDS             1990..3216
                     /gene="mdfA"
                     /locus_tag="ACHUQ7_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HFR1135246.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISFCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETAMRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARL
                     TSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGLVR
                     LTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWLSL
                     MVIFLKDKQMGNSHEG"
     gene            complement(3257..3541)
                     /gene="ybjH"
                     /locus_tag="ACHUQ7_001812"
     CDS             complement(3257..3541)
                     /gene="ybjH"
                     /locus_tag="ACHUQ7_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFR1135247.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(3627..4442)
                     /gene="ybjI"
                     /locus_tag="ACHUQ7_001813"
     CDS             complement(3627..4442)
                     /gene="ybjI"
                     /locus_tag="ACHUQ7_001813"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFR1135248.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVMSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGYNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(4442..5650)
                     /locus_tag="ACHUQ7_001814"
     CDS             complement(4442..5650)
                     /locus_tag="ACHUQ7_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1135249.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     SYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            5734..6270
                     /gene="rcdA"
                     /locus_tag="ACHUQ7_001815"
     CDS             5734..6270
                     /gene="rcdA"
                     /locus_tag="ACHUQ7_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFR1135250.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(6445..8130)
                     /locus_tag="ACHUQ7_001816"
     CDS             complement(6445..8130)
                     /locus_tag="ACHUQ7_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFR1135251.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLHHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            8400..8777
                     /locus_tag="ACHUQ7_001817"
     CDS             8400..8777
                     /locus_tag="ACHUQ7_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFR1135252.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(8807..9064)
                     /gene="grxA"
                     /locus_tag="ACHUQ7_001818"
     CDS             complement(8807..9064)
                     /gene="grxA"
                     /locus_tag="ACHUQ7_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFR1135253.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            9224..9511
                     /locus_tag="ACHUQ7_001819"
     CDS             9224..9511
                     /locus_tag="ACHUQ7_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFR1135254.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNCPPQYSRSEREKDNDANH"
     gene            9495..10217
                     /gene="nfsA"
                     /locus_tag="ACHUQ7_001820"
     CDS             9495..10217
                     /gene="nfsA"
                     /locus_tag="ACHUQ7_001820"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFR1135255.1"
                     /translation="MTPTIELICGHRAIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRWDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            10278..11180
                     /gene="rimK"
                     /locus_tag="ACHUQ7_001821"
     CDS             10278..11180
                     /gene="rimK"
                     /locus_tag="ACHUQ7_001821"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFR1135256.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIYYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            11268..11744
                     /locus_tag="ACHUQ7_001822"
     CDS             11268..11744
                     /locus_tag="ACHUQ7_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFR1135257.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            12096..13208
                     /gene="potF"
                     /locus_tag="ACHUQ7_001823"
     CDS             12096..13208
                     /gene="potF"
                     /locus_tag="ACHUQ7_001823"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFR1135258.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYVNANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            13303..14469
                     /gene="potG"
                     /locus_tag="ACHUQ7_001824"
     CDS             13303..14469
                     /gene="potG"
                     /locus_tag="ACHUQ7_001824"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFR1135259.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLR
                     PINMMFQSYALFPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQ
                     LSGGQRQRVALARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVT
                     HDQEEAMTMAGRIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQE
                     DGLVLDSPGLVHPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHI
                     AYLGDLSVYHVRLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            14479..15432
                     /gene="potH"
                     /locus_tag="ACHUQ7_001825"
     CDS             14479..15432
                     /gene="potH"
                     /locus_tag="ACHUQ7_001825"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFR1135260.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            15429..16274
                     /gene="potI"
                     /locus_tag="ACHUQ7_001826"
     CDS             15429..16274
                     /gene="potI"
                     /locus_tag="ACHUQ7_001826"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFR1135261.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     GGWSTRWYGELLRDDAMMSAVGSSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            16334..16822
                     /locus_tag="ACHUQ7_001827"
     CDS             16334..16822
                     /locus_tag="ACHUQ7_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFR1135262.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            16863..17990
                     /gene="rlmC"
                     /locus_tag="ACHUQ7_001828"
     CDS             16863..17990
                     /gene="rlmC"
                     /locus_tag="ACHUQ7_001828"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFR1135263.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(18165..18896)
                     /gene="artJ"
                     /locus_tag="ACHUQ7_001829"
     CDS             complement(18165..18896)
                     /gene="artJ"
                     /locus_tag="ACHUQ7_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFR1135264.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVISKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(19188..19856)
                     /gene="artM"
                     /locus_tag="ACHUQ7_001830"
     CDS             complement(19188..19856)
                     /gene="artM"
                     /locus_tag="ACHUQ7_001830"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFR1135265.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(19856..20572)
                     /gene="artQ"
                     /locus_tag="ACHUQ7_001831"
     CDS             complement(19856..20572)
                     /gene="artQ"
                     /locus_tag="ACHUQ7_001831"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFR1135266.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(20579..21310)
                     /gene="artI"
                     /locus_tag="ACHUQ7_001832"
     CDS             complement(20579..21310)
                     /gene="artI"
                     /locus_tag="ACHUQ7_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFR1135267.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEAPYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(21328..22056)
                     /gene="artP"
                     /locus_tag="ACHUQ7_001833"
     CDS             complement(21328..22056)
                     /gene="artP"
                     /locus_tag="ACHUQ7_001833"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFR1135268.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(22274..22789)
                     /locus_tag="ACHUQ7_001834"
     CDS             complement(22274..22789)
                     /locus_tag="ACHUQ7_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR1135269.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
BASE COUNT         5733 a         5755 c         6023 g         5812 t
ORIGIN      
        1 gtgaccatac cgcgtgggca gctgaaaaac ctgaaggcga gctacacgtt aacggaaccg
       61 cagcttaccg caccgctgaa aaaaggtcag gttgtcggga ccattgattt ccagcttaac
      121 ggtaaatcca ttgagcagcg tccgctgatc gtgatggaaa atgtggaaga gggcggattc
      181 tttggtagga tgtgggattt cgtgatgatg aaattccatc agtggttcgg cagctggttc
      241 tcttaatctt ctgataaccg gatggcgcga aacgtcatcc ggttatacgt cattaataca
      301 tcaacttaat acggtgcgtc tgcgcgtact tcacatactc atcttccggg caacaatcac
      361 tcaccacaat atcaaagcgt ttcaggtcac ccatgcgcgc cggacgcacc ttgccaaatt
      421 tactgtggtc gacaaccagc acatgctttt gcgccatcga catggcccag tgttttaccg
      481 gcaactcttc aagattaaaa caggtagcgc ctttactgac atgcacgccc gccgcggaat
      541 aaaaagcgat atccgggcaa aaattattca acgtttgctg aaaatcgatg ggtttgaaaa
      601 tggcgttgct ggcgtgaaat tcaccaccgc acagaaatgc gcggcaatgg ggtttctctt
      661 tcagcgccag aaaggtattt agcgaataac aaacggcggt aaaagggatt tcattatcaa
      721 tcgcttcaat aatccacggc gtggtggtgc cacagtcaaa aaagagggtc tgatcgggtt
      781 ctaccagtgt cgcagccagt tttgccgccc ggcgtttttc ttccaccagg cgggattttt
      841 gatcgcttaa caggtaatgg ctggcactgc gcggttccag aacaatatag ccgccgagca
      901 aaacgacggg cgcactgtgg ttgttcagat cgcgacgaat cgtcatctcc gaaaccccaa
      961 gcagggcggc ggcgtcttta agatgtaact tatcgctgcg ttttaattct tgcagcagct
     1021 gcccgatacg ctcttcgcga cgtgtttcca taatccctct gaatagttat tgaagcgagc
     1081 cgctcaatac tacacttttt agcagagatc agtcacgcac ccagcctttg cggatcggta
     1141 atgcaaaaca gacgcgatac cacgattgca gacgttgata gagtttatgc cccatcgctt
     1201 gccagataat ctgggcacta aggcagccaa tcatacctgc cagcaatcca ccgagcatat
     1261 ccagcggcca gtggacgcca agataaacgc gcgaccaggc aatgacgacg gccagcacca
     1321 ttaaaagtga gccggaccac aggcgatgcc agcataaaaa tgccagtgca aaggtgaaaa
     1381 tcaccgtacc gtgatcgctc gggaatgagt catccgccgc atgatgcagg aagttatagc
     1441 cgatattttc gacaaagggt cggtcgtgcg gaaaaagatg tcccatcgtc caggacacaa
     1501 acaggctgac cgccagcgcg atagcgattt ttatcaccag ttgccgttgt gctgtaagcc
     1561 cccacaacca aagtaccgcg gccagcaacg gcaccacggt aatcaaatct ttagcaataa
     1621 aaatcgccaa cgagatcatc cacggagccg agtctggcgt cgcgttaata agagagaata
     1681 gagagagatt caaattttcc agcataactt cccgacgcaa agtgattaaa agggagccaa
     1741 tacaggcaag tcgttgagaa taaagtgcag gttaaactgg gtaaagcggc atcgtcttat
     1801 ttccctcaag cggcctgttt acggtgggtg attgtaacgg gcataggtta aataaaactt
     1861 aaagaaagcg tagctatact cgcaataatg taagaatgtg cttaaccgtg gtttcagcta
     1921 caaaattcgc tttctcgtta gctgcgcttt tattaaactc tgcgcgatta ttattggcga
     1981 agaaattgca tgcaaaataa attagcttcc ggtgccaggc ttggacgtca ggcgttactt
     2041 ttccctctct gtctggtgct ttacgaattt tcaacctata tcggcaacga tatgatccaa
     2101 cccggtatgt tggccgtggt ggaacaatat caggcgggca ttgattgggt tcctacttcg
     2161 atgaccgcgt atctggcggg cgggatgttt ttacaatggc tcctggggcc gctgtcggat
     2221 cgtattggtc gccgtccggt gatgctggcg ggtgtggtgt ggtttatcgt cacctgtctg
     2281 gcaatattac tggcgcaaaa cattgaacaa ttcaccctgt tgcgcttctt gcagggcata
     2341 agcttctgtt tcattggcgc tgtgggatac gccgcaattc aggaatcctt cgaagaggcg
     2401 gtttgtatca agatcaccgc gctgatggcg aacgtggcgc tgattgctcc gctacttggt
     2461 ccgctggtgg gcgcggcgtg gatccatgtg ctgccttggg aggggatgtt tgttttgttt
     2521 gccgcattgg cagcgatctc ctttttcggt ctgcaacgag ctatgcctga aaccgccatg
     2581 cgtataggcg agaaactgtc actgaaagaa ctcggtcgtg actataagct ggtgctgaag
     2641 aacggccgct ttgtggcggg ggcgctggga ttcgttagcc tgccattgct ggcgtggatc
     2701 gcccagtcgc cgattatcat cattaccggg gagcagttga gcagctatga atatggcttg
     2761 ctgcaagtgc ctattttcgg ggcgttaatt gcgggtaact tgctgttagc gcgtctgacc
     2821 tcgcgccgca ccgtacgttc gctgattatt atgggcggct ggccgattat gattggtctg
     2881 ttggtcgctg ctgcggcaac ggttatctca tcgcacgcgt atttatggat gaccgccggg
     2941 ttaagtattt atgctttcgg tattggtctg gcgaatgcgg gactggtgcg attaaccctg
     3001 tttgccagcg atatgagtaa aggtacggtt tctgcggcga tgggaatgct gcaaatgctg
     3061 atctttaccg tcggtattga aatcagcaaa catgcctggc tgaacggggg caacggactg
     3121 tttaatctct tcaaccttgt caacggaatt ttgtggctgt cgctgatggt tatcttttta
     3181 aaagataaac agatgggaaa ttctcacgaa gggtaaaaaa atgcctgatt gatttgtgcg
     3241 atcaggcatt ctcgaattaa tggtgatggt cgtcaatctg gtgttcgata accatccctt
     3301 cacctacgct ggcaagatgg cgaacataag gatgcgggcg gtaagccgga gctggcgcag
     3361 gagccacata gacggtttgc ggcacagtcg cgacgctgac cgcttgtgga acgctgactg
     3421 aatcaggaac cacaaccact ttgtatccac tcggcacatc gacggtgaca ctttgcgcca
     3481 tcgccacacc agtaaagccc attaaaagcg cgcccaacag tagacaattt ttcataatca
     3541 tttcgcctga aataatgcag cgggttaatt tccgcgaatt atgcagcctt cattcaggca
     3601 ttgtgttgcc gggcgtgctg ccgtttttat tggtcaaatg gcgcttcgtg tttaagaact
     3661 ttatcgatca catccagtac gccttcacgg ttattgtagc ctgcccggta ttttgccgct
     3721 gcgacgactg cgctgccggc attttccatt gcaaaactaa agcctgcctg acgcagcatc
     3781 tcaatatcgt taccgccatc gccaaagacc accacttcgc tgtcgtctat tccccataat
     3841 ttctgcagtt ggcgaaggcc attggcttta tgtacgccgg ggataatcag atcgatgctg
     3901 ccgttgccgg tgtggactgg caccataata tcgccgatgg cctcatgtaa tgctttttgt
     3961 acttgtggaa tcagttcatc ggaaagattc aggccaaact taaagaaaat atcttctaag
     4021 ttgtcaaagt tatcgacgta ttccagacgg tgataataca tttccgccac cgttttcatg
     4081 gcatcgtcat attttttgag tgtataggcg ctattttttc cgcaggcaat aatttccact
     4141 tccgggcgcg tcagcaaatg ttccacgaca gtagcaaacg catccttcga cagctcgcca
     4201 ttaaaaacat ctttgccttc actcattacc cagccgccgt tttcagccac aaaggcaatt
     4261 tcattcgcaa tttcagggaa gaaagagatc aactgataat attgattccc gctggcgacc
     4321 acaaagcgaa tgccttgtgc tttcatttgc tgatactgag ccataaaccg ctcacggtta
     4381 taggtttttt gatcgcttaa gaaagtacca tccatgtcta ccgcaattaa tttaatgctc
     4441 atcaactatt ctccatcgcc gtctgcgttt tggtatcggg tttggcgacg gctttcgcga
     4501 caatagccgc gagaataacc agcgccagta caaccagcat tgcactacgt aatccataat
     4561 gttcgccgag atagcccagc agcggcggcc cgacgaggaa agccagataa ccggtcgttg
     4621 ctaccacact gacgcgggtt ggtgcatcgg ggccggtatc gctggcggca gaaatggtca
     4681 gcgggaagcc cagcgaggca cccagtcccc aaagtacaac agacactcct gcgacccagg
     4741 cgctatcgac aaaaataatc agcccaatac ccaacgcccc cattagtgca ctggcccgaa
     4801 ccacggcaac gcgactgtaa ctgtcgatga accaaccgcc ggtgaagcgt ccaacggtca
     4861 tccctagggt aaaaccggca taaatcagtg agccggaagt ggggctaaaa ccgtgaccat
     4921 caaccattaa taagggtaac cagtcgttgg cagaaccttc ggcaaaggcc atcgccagca
     4981 ctacaacacc aatcaacagc aactggatat cgcgataaaa aggaatgcct ttttcgccat
     5041 gctgggtgcc atcggcagca tttttgcccg taccgtcagg gattgcctga atggcgatat
     5101 aaataggcgc gatacctacc agcgccgcca ataaaatgtg caccgttgcc ggaacgccaa
     5161 aggccgtcag tgccatcccg acaccagcgc ctgccagcgt gcccaggcta taaaaaccgt
     5221 gcatcatcgg caaaaccgtt ttattcattt ctcgctcaac ggcggcacct tcaacgttta
     5281 tcgccacttc cgcagaacca aaacttgccc caaagacgcc gagaccaacg gcaaagagca
     5341 ggggcgatgt cagccagagt gccagactta atatcatcat cccgatcaat gcgcaggaca
     5401 tcgtgaccag gatgacatta cgtgtcccaa agcgtttcac taaccacgcc gagcagagaa
     5461 taccgctcat cgaaccgatc gacagaccaa agagaacacc gcccatttca gcgatcgaga
     5521 cagagagaat atcgcggata gcaggcgtac gggttgccca ggacgccatt aacaggcctg
     5581 gcaaaaagaa gaacataaac agcgcccagg ttcggcgttt caatgcatta cgtgaagaat
     5641 ttacggtcat agatcacgtc aaaataagaa gagggaagac aacattagca aggttgtgta
     5701 catttgtaca caattgcaga cagaggaaat gacatgcgtc gcgctaacga tccgcaacgg
     5761 cgagaaaaaa ttatccaggc cacgctggag gcggtgaaac tttacggaat acatgctgtt
     5821 acgcaccgca aaattgctac ccttgccggg gtaccgttgg gatcgatgac ctactatttt
     5881 tcaggaattg atgagttgtt gctggaggcg ttcagcagtt ttactgagat tatgtcccgg
     5941 caatatcagg cattttttag cgatgttagt gatgctcaag gcgcatgcca ggctatcacc
     6001 gatatgatct acagctcaca ggttgcaacg ccggataata tggagctgat gtaccagctc
     6061 tacgcgctgg ctagccgaaa accgctatta aaaacggtaa tgcaaaactg gatgcagcgc
     6121 agtcagcaaa cgctcgaaca atggtttgaa cccggaaccg cccgcgcgct tgatgcgttt
     6181 attgagggga tgacgctgca ttttgtcacc gaccgtaagc cgctatcgcg cgaggagatt
     6241 ttgaggatgg ttgagagggt tgcagggtag tagataaatt ttagataaca aaaaacccat
     6301 caaccttgaa ccgaaatggc ggggttgatg ggctccacaa aatggggaca tcaaagaaaa
     6361 gcagtggcac taattaagac tgatgccctg cggaaaagtt ctgcggttgt gcaaaaaaat
     6421 ttcattttca gggcaacttc agttttatcc taatcctggc cataccatga cgatgattgt
     6481 ccctgccagc gtcagcagga cgttggcgat tgcgtaggtg cccgcatagc ccagcgcagg
     6541 gatgttactg cgagctgtat cactgatgat ctccattgcc ggcgcgcagg tgcgtgcgcc
     6601 catcattgcg ccgaacaaca gtgcgcggtt cattcgcaat acataagcac cgaacaagaa
     6661 acagataacc acgggcacaa gactgacaat taatccggca atcaacatct gaccgccaat
     6721 cgcgcccagg ccgttattaa taccgctacc ggcgctcaga ccaacgcctg ccataaacac
     6781 catcaagccg aactctttca ccatgcttaa tgcaccttgc ggaatgtaac cgaaggtcgg
     6841 gtggttagca cgcataaagc ccagcataat tccggcgaat aacaacccgg cagcgttccc
     6901 catgccgaaa ctgaatgtgc tgaactggaa ggtgatcatc ccgatcatca gcccaataac
     6961 aaagaaggcg caaaatgcca gcaggtcagt gacctggctg tgaatcgaga taaagccgat
     7021 gcgatcggcg atggttttta cgcgacgggc atcaccgctg acttgtaaaa cgtcaccttt
     7081 gttaagcacg acgttgtcat ctatcggcat ctcaatctgg ctacgaatga cgcggttaag
     7141 gaagcaaccg tgatcggtca acttcagttg tgcgagacgt ttacctacag cgttatggtt
     7201 tttaacgacc acttcttcag tgacgatacg catgtcgaga aggtcacgat cgaaaacttc
     7261 tttaccgtta cggaagctgg gatcgagtcg ggcatgggcg tcgggatagc ctaccaacgc
     7321 tatttcatcg cccatttgta gcacggcatc accgtctgga tttgccagaa tcccgttacg
     7381 tcgaatacgt tcaatgtagc agccggtttg tcgataaata cccagttcac gcagattttt
     7441 gccgtcggtc caggccacca gctccgggcc gacgcgatag gcgcggatca ccggtaaata
     7501 aaccttacgg ttggcatcag tgtccaggcc acgttcgcgg gcgatttgct gggcgctggt
     7561 ctgtaagtcc tgatgctgca atttcggcaa gtaacgcgca ccaacaatca aactcaccag
     7621 accgattaaa taggttaagg catacccgag gctcagatta tccagtgcca gtgagagctg
     7681 cctgctttcc atgccggaat gatgcagtgt atcgccagca ccgaccagaa ccggtgtcga
     7741 cgtcatagag cctgctaaca taccggccgt caggccaata tcccagccaa acagcttacc
     7801 taaccctaag gcgatcacca gcgcactgcc aaccatcacc agtgctaaca ttaggtaatt
     7861 tttcccatcg cgaaaaaaaa tggaaaaaaa gttcggtccg gcttcgaccc cgacgcagaa
     7921 aataaacagc ataaagccaa gattaagcgc atcggtgtta atgctgaaat gttgttggcc
     7981 taataacagc gatacgacta aaacgccaat ggaattaccc agttggatcg aaccaagtcg
     8041 taactttccg agacatagcc caagcgcgag gaccacaaat aataacagaa tgtaattccc
     8101 atttaacaat tcggcgacgt ttatattcac ggaggctaac ttcttgttta ctagtaagct
     8161 gttgaaagaa atgctaattt acgataatgt gttttaccag aattcagggc gcagattcat
     8221 tcagcgcacc taaacgatag taaagtaaca atatatttta ctagtgtaat cacattaggt
     8281 atcaacggct atatgaattg cgttggccta tattagcatg gaatgcgaag cggctttatc
     8341 ttactgaacg ccacactggc gaaaaatgtg ttcgatagac gcagtgtcag gaggaacgag
     8401 tgaaacataa acaacgttgg gcgggggcaa tctgctgttt tgtcctcttc attgtggtgt
     8461 gcctttttct ggcgacgcac atgaaaggcg cttttcgggc tgccgggcat cctgaaatcg
     8521 gcttgctgtt tttcattctt cctggagcag ttgccagttt cttttcacag cgtagagaag
     8581 tcctgaaacc tctatttggc gcaatgctgg cggcaccctg ttcaatgctc attatgcggc
     8641 tgtttttttc accgacgcgc tcattctggc aagagctggc atggttacta agcgcggtgt
     8701 tctggtgtgc gctgggggca ctgtgtttct tatttatcag tagtttgttt aaaccacagc
     8761 acagaaaaaa tcagtaaagc cctcaacgcg agggcttgtc agacgatcag gcgtccagat
     8821 tttctttcac ccatgcagca aaatcggtat agccgccgat atgttgctga tcgacaaaaa
     8881 tctgcggcac ggtttctacg ggtttacctg ccttttgttg tagatcttct ttagtgatcc
     8941 cttccgcacg aatatctaca tactgatact gaaaatcatc gcgttcattg ctcaatttct
     9001 cagccagatc ttttgcacgc acacagtaag ggcaacccga acgaccaaaa ataacggttt
     9061 gcattatttc tctcctcata gatttatgcc tgtaatgatc acgctaaaat gtattcgctg
     9121 aaagtaggtt taacctgttg cattaattgc taaaagctat aactgttaaa cacaatacag
     9181 tgaaaagttt tagactgaag gctcactttg cagagggaag cgtatgcgcg cgatcggtaa
     9241 attgcctaaa ggcgtgttga tactggaatt tatcggaatg atgctactgg cggtggcgct
     9301 gctgtcggta agcgactccc tgtcgctgcc tgagccattt tctcggccag aagtgcagat
     9361 tctgatgatt tttctcggtg ttttgctcat gcttcccgct gcggtggtgg ttattcttca
     9421 ggtggcaaaa cgtcttgccc cacagctgat gaactgtcca ccgcaatatt cacgttcaga
     9481 aagagaaaaa gataatgacg ccaaccattg aacttatttg tggccatcgc gccattcgcc
     9541 atttcactga tgaacccatt tccgaagcgc agtgtgaggc gattattaac agcgcccgtg
     9601 cgacgtccag ttccagtttt ttgcagtgta gtagcattat tcgcattacc gacaaagcat
     9661 tacgtgaaga actggtgacg ctgaccggcg ggcaaaaaca cgtagcgcaa gcggcggagt
     9721 tctgggtgtt ctgtgccgac tttaaccgcc atttacagat ctgtccggat gctcagctcg
     9781 gcctggcgga acaactgttg ctcggtgtcg ttgatacggc aatgatggcg cagaatgcat
     9841 taaccgcagc ggaatcgctg ggattgggcg gggtatatat cggcggcctg cgcaataata
     9901 ttgaagcggt gacgaaactg ctgaaattac cgcagcatgt tctgccgctg tttgggctgt
     9961 gccttggctg gcctgcggat aatccggatc tcaagccgcg tttaccggcc tccattttgg
    10021 tgcatgaaaa cagctatcaa ccgctggata aagacgcact ggcgcagtat gacgagcaac
    10081 tggcggaata ttacctcacc cgtggcagca ataatcgctg ggatacctgg agcgatcata
    10141 tccgccgaac aatcattaaa gaaagccgcc catttattct ggattatttg cacaaacagg
    10201 gttgggcgac gcgctaaaac cgccacgtcg atgtatgata cgcgggcttt tgaccaggtc
    10261 tgacagagag gtgcagggtg aaaattgcca tattgtcccg ggatggaacg ctctattcgt
    10321 gtaagcggct gcgtgaagcc gctatacagc gcggtcacct ggttgaaatt cttgatccgc
    10381 tttcttgcta catgaacata aatcctgcgg cgtcttctat ttactacaaa ggccgcaagt
    10441 taccccattt tgacgcagtg atcccgcgta ttggcaccgc cattaccttt tatgggacgg
    10501 cggcactgcg ccagttcgag atgctgggga gctatccgct caatgagtcg gtcgccattg
    10561 cccgggcgcg tgacaaattg cgttccatgc aactgctggc gcgtcagggc atcgacctgc
    10621 ctgtcacggg cattgcgcat tcgccggatg ataccagcga tttaatcgac atggtcggtg
    10681 gtgcgccgct ggtggtcaag ttggttgaag gcacgcaggg aattggcgtc gtgctggcgg
    10741 agactcgtca ggcggcggaa agcgtgattg acgctttccg cggtctgaac gcgcatattc
    10801 tggtgcagga atatatcaaa gaggcgcaag ggtgcgatat ccgctgtctg gttgttggtg
    10861 atgaagtggt cgctgcgatt gaacggcggg cgaaagaggg ctattttcgt tccaatttgc
    10921 atcgtggcgg cgcggcaagc gtcgccagta tcacaccaca ggagcgtgaa atcgcgataa
    10981 aagccgcgcg aacgatggcg ctggacgttg ctggtgtgga tattctgcgt gctaatcgcg
    11041 ggccgttggt gatggaagtg aatgcgtcgc cggggctgga aggaatagaa aaaaccaccg
    11101 gtatcgatat cgcgggtaaa atgatccgct ggatcgaacg ccacgctacg acagaatatt
    11161 gcctgaaaac gggtggttag tcgcaatcac attactgatc atggttttgc ctgcgctttt
    11221 tgcgtaagct gtgccggtct ttttatcgaa agaggttgta caaaattatg acatcgctgg
    11281 tcgttcctgg tctggatacg ctgcgtcaat ggctcgatga cctggggatg agtttttttg
    11341 aatgtgataa ctgtcaggct ctgcatctgc cccatatgca gaatttcgac ggtgtctttg
    11401 atgccaaaat cgatctgatc gataacacga tcctgttttc tgccatggcg gaagtccgac
    11461 cttcagccgt attgccgctg gcggcggatt tatctgccat caatgccagt tcgctgaccg
    11521 tgaaagcatt tcttgatatg caggatgata atctgccaaa gctggtggtt tgccagtctt
    11581 tatccgttat gcagggcgta acctatgagc agtttgcatg gttcgtgcgt cagagcgaag
    11641 agcagatttc gatggtcatt cttgaagcta atgcccatca actgctgtta ccgactgacg
    11701 atgaagggca aaacaacgtt accgaaaact attttctcca ctgataattc ctttcgagca
    11761 cgcagtcgct gccgcagtga ctgcgcgctg caaaattatc tgctgttttt aaccttttct
    11821 taaagattat ttcacttctc ttgtgtcgat ttggctttat cacatagagc aaatacgcat
    11881 aaaaatttgt taaataccgt tttttaatcc gagctatagt ctcaaaccct ggctaaagtt
    11941 attcttgcga tgcttctata tagcgagcag tgctggccgg gagagagttc tcttttctta
    12001 caccgcgccg ataaaaaaat atgcacgttt attgcatatc tttcagtgtg acaacttttg
    12061 ttcgtttgtt aacgaacttt cagaaggaaa gagatgtgac cgccttaaat aaaaaatggc
    12121 tatcgggtct ggttgcgggt gctctgatgg ccgtctctgt cggcacgctc gcggctgaac
    12181 aaaaaacact ccacatttat aactggtctg attatatcgc ccctgacacg gtggccaatt
    12241 ttgaaaaaga aaccggtatt aaagtcgtct acgatgtttt cgactctaac gaagtactgg
    12301 aaggcaaatt aatggcgggt agcaccggct ttgacttggt ggttccatct gccagctttc
    12361 tagaacgcca gttgactgcg ggagttttcc agccgctgga caaaagcaaa ttgccggagt
    12421 ggaagaatct cgacccggag ctgctgaagc tggtcgccaa acacgatccc gacaataaat
    12481 ttgctatgcc ctatatgtgg gcgacgaccg ggattggcta taacgttgat aaagttaaag
    12541 ctgtgctggg cgaaaacgcg cccgtcgata gctgggactt gatcctcaaa cctgaaaatc
    12601 tggaaaaact gaaaagctgc ggtgtctctt tcctggatgc gccagaagaa gtttttgcta
    12661 ccgtgttgaa ttatctcggc aaagatccca acagcactaa agcggatgat tacaccggac
    12721 cggcaaccga tctgctgtta aagctgcgcc cgaacattcg ttatttccat tcatctcaat
    12781 acattaacga cctggcaaac ggcgatattt gcgtcgctat cggctgggca ggtgatgtct
    12841 ggcaggcgtc aaaccgcgcg aaggaagcga agaatggagt gaatgtctcg ttctcgattc
    12901 caaaagaagg ggcgatggcg ttctttgatg tattcgccat gccagcggat gccaaaaaca
    12961 aagacgaagc ctatcagttc ctgaattacc tgctgcgccc ggatgtagta gcgcatattt
    13021 ccgaccatgt gttctatgtc aacgccaata aagcagccac gccgctggtg agtgcggaag
    13081 tccgtgagaa cccgggtatt tatccgcctg cggatgttcg tgcgaagctg ttcactctga
    13141 aagtgcagga cccgaaaatc gaccgtgtgc gcacccgcgc gtggaccaaa gtgaagagcg
    13201 gaaaataatc cgcagcctta gatgccgggc gggcgcacca cacccgccgg caattcgcac
    13261 catcatggtg cgcttgcaca cattcaatgc cggagagcag ccgtgaatga cgctatccct
    13321 cgcccgcagg cgaaaacccg taaggcgctg acgccgctat tagaaatccg caacctcacc
    13381 aaatcctacg atggtcaaca tgcggtggat gatgtcagcc tgaccatcta caaaggtgaa
    13441 atcttcgcgc tgctgggcgc atccggctgt ggcaagtcca cgctgctggg cgcatccggc
    13501 tgtggcaagt ccacgctgct gcgtatgctg gcaggtttcg aacaaccttc tgccggacag
    13561 ataatgcttg atggcgtcga tttgtcacag gttccgcctt acctgcgccc catcaatatg
    13621 atgtttcagt cttacgcgct gtttccgcat atgaccgtgg aacagaacat cgcttttggc
    13681 ctgaaacagg acaaactacc gaaagcggaa atttccagtc gggtcaatga gatgctcggc
    13741 ctggtgcaca tgcaggagtt cgccaaacgt aaaccgcatc agctttctgg tggtcagcga
    13801 cagcgtgtgg ccctggcccg aagccttgcc aagcgcccga aactattact gctcgacgag
    13861 ccgatgggcg cgctggataa aaagctgcgt gaccggatgc agcttgaagt ggtggatatt
    13921 ctggagcgcg tcggtgtgac ttgtgtgatg gtcacccacg atcaggaaga ggcgatgacc
    13981 atggcggggc gcatcgccat tatgaatcgt gggaaatttg tccagattgg cgaaccggaa
    14041 gagatctacg agcatccgac tacccgctat agcgccgaat ttattggctc ggtaaacgtc
    14101 tttgaaggcg tactcaaaga gcgtcaggaa gatggcttgg tgcttgattc gccggggctg
    14161 gtgcatccac tgaaagtcga cgcggatgcc tcggtggtcg ataacgtgcc ggtacatgtg
    14221 gcgctgcgcc cggaaaaaat catgctttgc gaagagccgc ccgccaatgg ttgtaacttc
    14281 gcggtggggg aggtgataca cattgcctat ctcggcgatc tttcggtgta tcacgttcgt
    14341 ctgaaaagtg ggcagatgat tagtgcccag ctacaaaacg cccatcgtca tcgtaaaggg
    14401 ttaccgacct ggggcgacga agtgcgtttg tgctgggaag tggacagctg tgtggtgctg
    14461 acggtttaag gagcaaagat gaatacactt gaacctgctg cccagtcgaa accgccgggc
    14521 ggatttaagc tgtggttgtc gcagctgcaa atgaagcatg ggcgcaaact ggtcattgcg
    14581 ttgccatata tctggttgat cttgctgttt ctgctgccat ttctgattgt ctttaaaata
    14641 agcctggcgg agatggcgcg cgctattcca ccttataccg aactgatgga gtgggctgac
    14701 gggcaactgt ccatcactct taatctcggt aattttctgc aactgaccga cgatccgctc
    14761 tatttcgatg cttatctcca gtcgctacag gtggctgcga tttcgacatt ttgctgttta
    14821 ctgatcggct atccgctggc gtgggcggtg gcgcacagta agccttcgac ccgtaatatt
    14881 ttattactac tggtgatcct gccgtcgtgg acctcgtttc tgatccgcgt ttatgcttgg
    14941 atgggaatat taaaaaacaa cggtgtgctg aataattttc tgctgtggct gggggttatc
    15001 gatcaaccgc tgaccattct tcataccaat ctggcagttt atattggcat tgtttacgcc
    15061 tacgtgccgt ttatggtgct gccgatttat accgcgttga ttcgtattga ttattcgctg
    15121 gtggaagcag cgctggatct cggtgcacga ccgctgaaaa cgttctttac tgtgatcgtg
    15181 ccgctgacta aaggtgggat tattgccgga tcgatgctgg tgtttatccc ggctgtgggc
    15241 gagtttgtga tcccggaact gctcggtggc ccggacagca tcatgatcgg gcgcgtgcta
    15301 tggcaagagt ttttcaataa ccgcgactgg ccggtggcct cggcggtagc gatcatcatg
    15361 ttgctgctgc taattgtgcc gataatgtgg ttccacaaac accagcaaaa aagcgtggga
    15421 gaacacggat gaataattta ccggtagttc gttcgccctg gcggattgtg attttgctgc
    15481 tgggctttac ctttctctac gcgccaatgc tgatgctggt tatctattcg tttaacagct
    15541 cgaagctggt gacggtgtgg ggcggctggt caacgcgctg gtatggtgag ttattgcgcg
    15601 atgacgcgat gatgagtgcg gttggttcaa gcctgacaat tgctgcctgt gcggcaacgg
    15661 cggcggcgat cctcgggact attgcggcgg tggtgctggt gcgctttggc aggtttcgcg
    15721 gatcaaatgg ctttgccttt atgatcaccg cgccgctagt aatgccagat gtcatcacgg
    15781 gcttgtcgct gttgttatta ttcgtcgcgc ttgctcatgc cattggctgg cctgcggacc
    15841 gcggtatgct taccatctgg ctggcgcatg tcactttctg tacggcttat gtggcggtcg
    15901 ttatttcgtc gcgtctgcgg gaactggatc gctcgataga agaagccgcg atggatctcg
    15961 gtgcgacgcc gctgaaagtg tttttcgtca ttacgctacc gatgatcatg cccgcgatca
    16021 tttctggctg gttactggct tttactttgt cgcttgatga tctggtgatc gccagctttg
    16081 tttccgggcc gggagccacc acgttaccga tgctggtctt ttccagcgtg cggatggggg
    16141 tgaatccgga aatcaacgcc ctggcaacat tgatcctcgg cgcggtcgga attgtcggat
    16201 ttatcgcctg gtatctgatg gcgcgcgcag aaaaacagcg gatacgcgac atccaacgtg
    16261 caagacgtgg ctgaagacac taaaatttgc caacctggct acataatgcc gcgcatgtcg
    16321 cggcattgtt ttcatggaag acgaaacgtt gggatttttt aagaaaacat cttcatctca
    16381 tgctcgcctg aatgtgcctg cgctggtgca ggtggcggcg ctcgccatta ttatgatccg
    16441 tggcctcgac gtgctgatga ttttcaatac gctgggcgtg cgcggtattg gcgagttcat
    16501 tcatcgcagc gtacaaacct ggagtttaac gctggtcttt ttaagcagtc tggtgctggt
    16561 tttcattgag atctggtgtg cgttttcact ggtgaaaggg cgtcgctggg cgcgctggct
    16621 atatctgctg acacaaatca ccgccgcaag ttacttgtgg gcggcttcgc tggggtacgg
    16681 ttatccggag ctgttcagca ttcccggtga atcaaaacgt gaaatcttcc atagcctgat
    16741 gctgcagaag ctgccggata tgctcatcct gatgctgctg ttcgttccct cgaccagtcg
    16801 gcggttcttc cagttgcaat aatgtgtata atcgtcgccc ctgatgatgt gaaggtcaat
    16861 gtatgcagtg cgcactttac gacgcgggtc gctgtcgttc ctgtcagtgg ataacgcagc
    16921 cgattccaga gcaactctcc gctaaaaccg ccgatcttaa aaatctgctc gccgattttc
    16981 cggttgagga atggtgcgcg ccggtgtcag gcccggaaca agggtttcgt aataaagcca
    17041 aaatggtggt gagtggtagc gttgaaaaaa cactgctcgg tatgctgcat cgcgatggca
    17101 cgccggaaga cctttgtgac tgcccgcttt atccagcctc atttgcgccc gtttttgcgg
    17161 cgctaaaacc gtttatcgcc cgagcggggt taacgcccta caacgtggcg cgtaagcgtg
    17221 gcgaactgaa atacattctg ctgactgaaa gccagagtga tggcggcatg atgctgcgct
    17281 ttgtactgcg ttctgatacc aagctggcgc aactgcgtaa ggcgctgccg tggttacacg
    17341 aacaactacc gcagctgaaa gttattaccg tcaatattca gccggtacat atggcgatta
    17401 tggaagggga gacggagatc tacctgaccg aacaacaggc actggcggag cgttttaatg
    17461 acgtaccgct gtggatccgt ccgcaaagtt tcttccagac taatccggcg gtcgccagcc
    17521 agttgtacgc caccgcgcgc gactgggtac gacagctgcc ggttaaacat atgtgggatc
    17581 tgttctgcgg tgtggggggc tttggtttac actgcgcgac gcctgacatg cagttaaccg
    17641 ggatcgaaat tgcgccagag gccattgcct gtgcaaagca gtcagccgct gaactgggct
    17701 taactcgttt acaatttcag gcgctggact ccactcagtt tgccactgct cagggggagg
    17761 tgccggagct ggtgctggtt aacccgccgc gccgcggcat tggtaaaccg ctgtgtgatt
    17821 atctttcaac gatggcaccg cgttttatta tctactccag ctgtaacgcc caaaccatgg
    17881 cgaaagatat ccgcgaactg ccaggttacc gcatcgaacg ggtacagctt tttgatatgt
    17941 tcccgcacac cgcgcactat gaagtgttga cgctgctggt gaagcaataa aaaagccaca
    18001 ggtgcggctt cagattgctg atcaggtgcg ctttgttcat gccggatgcg gcgtgaacgc
    18061 cttatccggc ctacaaaatc atgctaaatc aatacattgc agagttgatg tgggccggat
    18121 aagcgcagcg catcaggcaa tcttactttt gtcatcagtc tcaattactg cggaaaccac
    18181 tggtcattga ttttctgata tgtaccgtca gctttaattg ctgccagcgc gttattcagt
    18241 ttttccagca gagctttgtt atccggacgt acagcgatac ccaggccggt gccgaagtat
    18301 tgcggatcgg tcactttttc agtcgcaacg cccagttgcg gattggtttt cagccattcg
    18361 tttaccaccg cggtgtcacc aaatacccca tcaatacgac catttttcag atcgataaag
    18421 gcattctgat aactgtcata ggagacagtt ttcacttccg ggtgctgatc ctgaatatat
    18481 ttctggtgcg tcgtaccgtt ttccatccca atgcgtttgc ctttcaggtc ggcaaacgtt
    18541 ttgtaggtat cttttttgga aatcacgacg gctgagtttt catagtaggg tgtggtaaac
    18601 gaaacctgct tgctacgctc cggggtgata tccataccgg agattacggc gtcatatttt
    18661 ctgaatttca gtgacgggat caggctgtcg aacgcgtgat tagtaaaagt acattctgcc
    18721 tgcatttgtt tgcacaaggc ttttgccaga tcgatatcaa agccgacaat ctcattatta
    18781 gcacctatag attcaaaggg tggataggtg gctgaaacgc caaaattgat tttctctgcg
    18841 gcagaagcac cgaaagtaaa ggaagcaagt aaagcggcaa gaactaactt tttcatgatg
    18901 gaactcccgt ctgtcaatct tatgattttt ggccgtgtct gcggcatggg ataacaatgc
    18961 catcaagtga atttatatgc aataaacatg attaaataat ttaaatgaaa taaaaaaaga
    19021 cggataactt agtgagttgt ccgtcttcat tataagaatt tatgcactat gtaggccgga
    19081 taaggcgtcc acgccgcatc cggcacaggc accgtgctga tgtctgatgc gacgctggcg
    19141 cgtcttatca gacctacaaa cccccccggc gaatgtacgc agctacatta attccgccgt
    19201 tcgaatgcca gtgctttgcg ctcgatcaga cgcatcatca gcgtcagcag gccgttgacg
    19261 accaagtaaa taatccctgc cgcaccgaac accattacat cgtaggtgcg tccgtacaac
    19321 aactggctgt atcccatcac ttccatcagc gtaatggtgt aagccagaga ggtacttttg
    19381 aacaccagca ccacttcgtt ggaataagaa gagagcgagc gtttaaaggc atacggcagc
    19441 aggatcgcca gcgtatcttt tttgctcatt cccagggcgc tacaggactg ccactgacct
    19501 tctgggatcg cacgaattgc accgtaaaac agctgcgtgg tatacgccgc actgttcagc
    19561 gataacgcaa tcagcgcaca taaccacggt tctgacaaca aatgccacag tgccggatac
    19621 tcctgcaacg tcggaaactg gcccggcccg taataaatca ggaagatctg caccagcagc
    19681 ggcgtaccgg taaacagcgt gatataaccc cgcaccagcc acaccagcac cggcgttttc
    19741 agcgtcagga tgatggtaaa aatcaatgcc agaatcagtg ccacaatcag cgaggcaacg
    19801 gttagcgtca ggctggtgtg cagccctttc atcagctcgg gtaaatactc aaacattagc
    19861 tgggcctccg ctcaaaacgt gtcgcgcgca ggtcaatgcg tttgagaatg tactgactga
    19921 gcagggtgat caccaggtaa atcgccgccg ccacaatgta ccaggtaaat ggttcctggg
    19981 tacgagtagc aatgcttttg gtttgtaaca ttaaatcatt cacactaatc aaactgacca
    20041 gcgcggtatc tttcagcagc accagccact ggttaccgag gccaggcagc gcatgacgcc
    20101 acatctgcgg catcaccaga cggaaaaaga tagccgattt cgacagcccc agcgcctgac
    20161 cagattccca ctggcccacc ggtacagctt tcaacgcgcc gcgcagcgtt tgcgaggcat
    20221 aggcggcata cagcagtgac agagcgatga caccacaaag gaacggactc acgtcgaagt
    20281 tctcaatgtc catctgcacc gggatctgca cgaacccaag attgatagtg aagccatccg
    20341 aaagcgtcag cagtaactgc gaggagccaa aatagataaa cagcactacc agaatttctg
    20401 gcaggccacg cagaatggtt accagcgctg aacctgccca cgcgacagga cgccatttcg
    20461 ctgactccca taccgcaaag aacatcgcca gcgccagccc gacaatcaat gcacaaacgg
    20521 caaggccgac ggtcatcccg gcggcgcttg ctaaaggaaa aaattcattc atcaggaatt
    20581 acttctggaa ccatttgttg tagatggttt cgtaagtgcc atctttcttc actttttcca
    20641 gcgcagtgtt gagtttctgc tgcagttcag tgtttccctg acgtaccgcg atgccgaggc
    20701 cagtaccgaa ataatcttta tcggtcactt tatcgcccac tgccgccagt tttgggttat
    20761 ctttcagcca ctcagtgacc actgcggtgt caccaaatac gctgtcgata cgcccgtttt
    20821 gcagatccag ttttgcgttc tggtagctgt catacggaac ggtggtgatt tccgggtgct
    20881 tatccataat gaacttctgg tgtgtcgtcc cgttctgaac gccgactttt ttgcctttca
    20941 gctgatcaac actggtgtat ttgccttgct gacccacaaa cagggcagag ttgtcatagt
    21001 acggggtagt aaacagtacc tgcttttcac gctccggagt gatatccatg cccgccatca
    21061 ctgcttctac gcgacggaat ttcaggcttg ggatcaggct gtcaaacgcc tggttagaga
    21121 aagtacaggt tgcatcaatc tctttacaca gtgcctgcgc caggtcgacg tcaaaaccaa
    21181 cgatctggtt gtttgcatca atcgattcaa acggaggata gggggcttcg gtagcaaaac
    21241 gaatggtttc ggcagctgtg gcggaaagac taaaacctgc aattaacgcg gcaatcagaa
    21301 cttttttcat tgttgttatc ccgaatctta gtgagagaga tagtttttaa atgcttcggt
    21361 ttgcggctcg gtaaagcagc tcgcgtcgcc ttgttctacg atatgaccat tttccatata
    21421 caccactcgg ctggcggttt tacgcgccac ttcaacttcg tgggtgacga tcacctgggt
    21481 aatattcgtt tctgccagct cacgaatgat gctgacgatt tgtgccgtaa tttccgggtc
    21541 cagtgcggcg gtcggttcat cgaacagcag tacctgcggt tccatcatca acgcacgggc
    21601 aatagcaaca cgctgctgct gaccaccaga aagatgcagc gggtaacgat cgctataagg
    21661 tttgagacgc agacgttcca gcagtttttc tgcacgggcc agcgcctgat ctttactcaa
    21721 ccccagtaca cggcagggcg cttcaatcag gttttgctgc acggtcagat gcggccacag
    21781 gttgtattgc tgaaacacca tgccaacgtt acgacgcaaa tcgcgaatcg ctttgtcaga
    21841 gggtgttttg gtgaaatcga aatggttgcc tgcaatgttg agcgtaccgg agcgcggcat
    21901 ctcaagcaga ttgagtacac gcagcagcga gcttttaccc gcgccgctgg ggccaagtaa
    21961 caccagcgtt tcgccctgtg ggcaatccag cgtgatatcg aacagcgcct gatgcgcgcc
    22021 gtagaagcaa ttaatgccgt ttaattgaat actcattgac actcgtatac tggcagtctg
    22081 atagctattg aggtcgaaga tagtaccttt aacagaataa ttatgcaata tttatgcttt
    22141 aaaagttaaa agcaaagcgc attattcaat aaacatagca caaaataacg ggggcggtgg
    22201 tcggtgagca taaatgtcgg cattcctcac gaaatgccgg ataatttacg gggtttattg
    22261 gttgatcaag gcgttagcga ttctcgatgg actgacggag cgtacccgcc gtggcatgaa
    22321 cgctaccgcc taagtagcgc acatcgtcga tgacccaaca ctggccttcc tgaatcatta
    22381 acacttcatc ctgccagccc tggtcaccct gtttgagatc cacccgcaat ggaatgttac
    22441 gggcatcacg attagggata gtcgatgcac tggcaacgtg ggcgctgtct ggcaaggtgg
    22501 ttcgactgga gaacggatcg ctgcttaaca attcacgatg actattatcg cggctggcat
    22561 cgctaagttg ggttgccagt ttgtcactca aataggggcg cagggcggta atgtcattgc
    22621 tgcgatgttg aatgcggtag tcgtagaatt gttgagccac actgtcaggc cctccctgga
    22681 cacaggaacc ggtgcggggg ccgttatctt tataagctgg cgtgacggtg gtacaggcac
    22741 tcagcagcag tgcacatgga ataagtagag atagtgaacg gtagcgcata atcatttcct
    22801 tattagcttg ctgtctgcgt cacactacga ccaaacgcta tcagattatt taatacattg
    22861 tttttaatac aattctgaaa caatctgaac acctgattgg gtttgagtcc attgctggcg
    22921 acccactcaa ggttgctcgt agctgactta cttaaattac ccaaacaata gacagtgtaa
    22981 accatttgtg aggttgagcg tgttatatgg ctgaaatgat catttaaggt aaatatgata
    23041 gttctgaaat ggtggtataa atcctcctgt agaggttaag agcatctgca agaagccagt
    23101 ttcacattta taacgcgtga taaatgaatg cggttattgt ctaatcatta caaaattttt
    23161 cacatgcgat aagagatcct gcgattaaga tgattgccaa aattacgttg tatttttgaa
    23221 aatgagcgaa atttgataga acatttatct gctgtacgat ttaatgaact taatgataca
    23281 gcattacaaa aacaatcgaa gtttataaag atgatttctg att
//
LOCUS       DAZPCS010000046        23021 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZPCS010000046 DAZPCS010000000
VERSION     DAZPCS010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23021
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.046"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            25..954
                     /gene="aaeR"
                     /locus_tag="ACHUQ7_001835"
     CDS             25..954
                     /gene="aaeR"
                     /locus_tag="ACHUQ7_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFR1135270.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            complement(1082..2527)
                     /gene="tldD"
                     /locus_tag="ACHUQ7_001836"
     CDS             complement(1082..2527)
                     /gene="tldD"
                     /locus_tag="ACHUQ7_001836"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFR1135271.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(2683..6483)
                     /gene="yhdP"
                     /locus_tag="ACHUQ7_001837"
     CDS             complement(2683..6483)
                     /gene="yhdP"
                     /locus_tag="ACHUQ7_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFR1135272.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLNAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSILKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            complement(6551..8020)
                     /gene="rng"
                     /locus_tag="ACHUQ7_001838"
     CDS             complement(6551..8020)
                     /gene="rng"
                     /locus_tag="ACHUQ7_001838"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFR1135273.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            complement(8010..8603)
                     /gene="yhdE"
                     /locus_tag="ACHUQ7_001839"
     CDS             complement(8010..8603)
                     /gene="yhdE"
                     /locus_tag="ACHUQ7_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFR1135274.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            complement(8612..9100)
                     /gene="mreD"
                     /locus_tag="ACHUQ7_001840"
     CDS             complement(8612..9100)
                     /gene="mreD"
                     /locus_tag="ACHUQ7_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFR1135275.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            complement(9100..10203)
                     /gene="mreC"
                     /locus_tag="ACHUQ7_001841"
     CDS             complement(9100..10203)
                     /gene="mreC"
                     /locus_tag="ACHUQ7_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFR1135276.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            complement(10269..11312)
                     /gene="mreB"
                     /locus_tag="ACHUQ7_001842"
     CDS             complement(10269..11312)
                     /gene="mreB"
                     /locus_tag="ACHUQ7_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFR1135277.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            complement(11617..13557)
                     /gene="csrD"
                     /locus_tag="ACHUQ7_001843"
     CDS             complement(11617..13557)
                     /gene="csrD"
                     /locus_tag="ACHUQ7_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFR1135278.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            13709..14683
                     /locus_tag="ACHUQ7_001844"
     CDS             13709..14683
                     /locus_tag="ACHUQ7_001844"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFR1135279.1"
                     /translation="MQALLLEQQDGKTLASVQTLDERRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASGILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            15661..16131
                     /gene="accB"
                     /locus_tag="ACHUQ7_001845"
     CDS             15661..16131
                     /gene="accB"
                     /locus_tag="ACHUQ7_001845"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFR1135280.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAKSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            16142..17491
                     /gene="accC"
                     /locus_tag="ACHUQ7_001846"
     CDS             16142..17491
                     /gene="accC"
                     /locus_tag="ACHUQ7_001846"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFR1135281.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSTDRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            17600..17842
                     /locus_tag="ACHUQ7_001847"
     CDS             17600..17842
                     /locus_tag="ACHUQ7_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFR1135282.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            17832..19283
                     /gene="panF"
                     /locus_tag="ACHUQ7_001848"
     CDS             17832..19283
                     /gene="panF"
                     /locus_tag="ACHUQ7_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFR1135283.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIIVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            19295..20176
                     /gene="prmA"
                     /locus_tag="ACHUQ7_001849"
     CDS             19295..20176
                     /gene="prmA"
                     /locus_tag="ACHUQ7_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFR1135284.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            20505..21470
                     /gene="dusB"
                     /locus_tag="ACHUQ7_001850"
     CDS             20505..21470
                     /gene="dusB"
                     /locus_tag="ACHUQ7_001850"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFR1135285.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            21496..21792
                     /gene="fis"
                     /locus_tag="ACHUQ7_001851"
     CDS             21496..21792
                     /gene="fis"
                     /locus_tag="ACHUQ7_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFR1135286.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            21878..22762
                     /gene="yhdJ"
                     /locus_tag="ACHUQ7_001852"
     CDS             21878..22762
                     /gene="yhdJ"
                     /locus_tag="ACHUQ7_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFR1135287.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDAFAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            22846..>23021
                     /locus_tag="ACHUQ7_001853"
     CDS             22846..>23021
                     /locus_tag="ACHUQ7_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFR1135288.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDR"
BASE COUNT         5547 a         6162 c         5805 g         5507 t
ORIGIN      
        1 atcactttta agtcagagtg aataatggaa cgactaaaac gcatgtcggt gtttgccaaa
       61 gtagttgaat ttggctcttt taccgccgcc gccagacagc tacagatgag cgtttcgtcc
      121 atcagtcaga cggtgtcaaa actggaagat gagttgcagg taaagctgtt aaaccgtagc
      181 acacgcagca ttggcctgac cgaagccggt agaatttact accagggctg ccgtcgtatg
      241 cttcatgaag tgcaggatgt tcatgagcaa ctgtatgcct tcaataacac ccccatcggg
      301 acgctacgca ttggctgttc ttcaactatg gcacaaaatg ttctcgccgg gctgacagcc
      361 aaaatgctga aagaataccc aggtttgagc gccaatctgg ttaccggaat tccagccccc
      421 gacctgattg ccgacggtct ggatgtggtg atccgcgtcg gcgcgttgca ggattccagc
      481 ctgttttccc gccgtcttgg cgcgatgcca atggtggtgt gcgccgcgaa aagctatctc
      541 acacaatacg gcataccgga aaaacccgcc gatttaagta gccattcatg gcttgaatac
      601 agcgtgcggc ccgacaatga atttgaactg attgcaccgg aagggatctc gactcgcctg
      661 atcccgcaag ggagatttgt gactaatgat ccgatgacgc tggtgcgctg gctaacggcg
      721 ggtgccggga tcgcctacgt gccgctgatg tgggtgatca acgagatcaa tcgtggggag
      781 ctggagatcc tgctgccgcg ttatcagtca gatccacgcc cggtttatgc gttatatacc
      841 gaaaaagata agctgccgct gaaggtacag gtcgtgatca actcgctgac ggattatttt
      901 gttgaggtcg gtaaattgtt tcaggagatg cacgggcgcg ggaaagagaa gtaattcata
      961 ttgtactgtt acgttctaca aacctgtgcc aacgggttcc cctcacccta accccagagg
     1021 agcgagggga ccgttcgtgc acgtagaaag attaattatc cttctgaaaa taatgaaatt
     1081 attacgcagt accgccaaca gtcaggttat cgactttcaa cgttggctgg cccacgccaa
     1141 ccggcaaact ttgcccttct ttaccgcaga cacccacgcc gttatccagt ttcaggtcgt
     1201 tgccaaccat cgaaatctgc tgcatggttt cgataccgga accaatcaac gttgcgcctt
     1261 tcaccggctt cgttacttta ccgttttcaa tcagatatgc ttctgaagtg gagaaaacga
     1321 atttgccgga ggtgatatcc acctgaccgc caccaaagtt cggtgcatag ataccgtact
     1381 caacggattc aataatttct tgcggggtcg atttacccgg cagcatatag gtgttggtca
     1441 tacgcggcat gggcagatgg gcgtaggatt cacggcgacc gttgccagtc ggcgtcatcc
     1501 ccatcaaacg cgcgttgagt ttatcctgca tgtagccttt cagaatgccg ttctcaatca
     1561 gcacgttgta ctggcctggc gtaccttcat catcaatcgc caccgaacca cggcgatcga
     1621 ccatcgtgcc gtcatcaacc acggtacaca gttctgaagc caccagctcc ccgacctgtc
     1681 cactaaatac tgaagtgcca cggcggttga agtcgccttc cagaccgtga cctaccgctt
     1741 catgcaacag cacgcccggc caacctgcgc caagtactac cggcatggtg cccgctggtg
     1801 cagcaacggc agaaagattg accagcgcca tacgcactgc ttcttttgcc catgcatccg
     1861 cacggacttc gccgtcaaga tcggcaagga agaactcata accaaaacga ccgccgccgc
     1921 cactggcacc gcgttcgcgt ttgccatctt cttcgaccag aacgctcacg gaaagacgca
     1981 ccagcggacg gacatccgcc gctagcgtgc cgtcggtggc cgcaaccaga attaattcat
     2041 agacaccact gaggctggca gtcacttcct gtacgcgctt gtccgcttcg cgagcaacct
     2101 tatcgacgcg acgcaggata tccagcttct cttcacggct catgctttgc agcggatcta
     2161 ccgaggtata caacgggcta tgctctaccg cgcccagcgt ctgtactttg ccatcaccac
     2221 tatcacggac gatggtgcgc gccgcttgcg cactctgttc cagcgccagc aggctgattt
     2281 ggtcagcgta agcaaatccg gttttttcac cgctgattgc acgcacgcca acgccctgat
     2341 cgatgttgta agaaccatct ttaataatgc ggtcttctaa aacccaggat tcgtgatagc
     2401 tcgactgaaa atagagatcg ccataatcaa ggcgacgttc ggccagttga ccgaggatcg
     2461 cgaacaagtc ctgatgtttc aggccgttcg ccgctagcaa ttgttcactt accaggttca
     2521 gactcatcgt ttttgctact cgttagttac tgcagtagag gattttttac ggctgccgga
     2581 tgcggcgtga acgccctatc cggcctacag ttatgttccg gctcgttggc ctttggcaac
     2641 gattatcata tgagattggg gcaattacgc gccctcgtca aatcattgcg ctttttcttt
     2701 acgcggttgg cgcaacactt cgttgatttg cggatcgtcc agcggacccg aaatgtgata
     2761 gcgcaaaatg gagactttgc tccacagcgg ccccagcact ttactggcgg caaacactgc
     2821 cgcgccaaca atggggttaa ccgcaaaagc cgcagccacg cccaccgtcg cagaaatctc
     2881 tggtgcgaca accgcttcca tattcaggtc gcggcgtacc agatttaccg accctttcat
     2941 ggcgatatcc gcctccaggc catccaccag cgtgtcgtcg gtgtgcataa cgccgtcttt
     3001 aatccacgcg gtgctgcgaa tggagtcaaa atagaaccct tcgccaaaag tgtctctgaa
     3061 atcaaaacgc agcttacgca tcagggcatc tacgctcaat aagcgcagca attgcccggc
     3121 atgtccggta ttgatttcgg taatttcgcc tttacccagt tgagtatgaa tgatgccatt
     3181 caacgtcgct tcatctggct gccagggtgc tttgcgccag tgtaaatcgt aatccacatt
     3241 aaatgacgac tggcgtatgg gcgtcgtgac accaaaaaat tctgcggcgg catcaatttt
     3301 ctgcccacgc agttttcctt tcagcgaggt acgttcattc cccggattat taacccattc
     3361 accatcggca gtaagccgcg agaaaccagt atcaatcagt ccattggtca gcgttaacgt
     3421 atcgccagaa atggtgatat cactgtcaat gcgaccgaat ttttgccccc agaaccagca
     3481 ctctgtgcat cgtatttgtg cgtccggcca gccacggaag ttaatgcgct ccgttgtcgg
     3541 gaaaggtgat gacggcgttg aatcaccacg agttttcgcc acgctcgggt tgtaataaag
     3601 atatttgata ttcgccagcc acggcgcgtt attacgcatc gctagcgtgg cgttgatttc
     3661 acgcccttgc gcctcaacca gggtgccatt tgccgtcggt tgcgaaacaa tactcaggtt
     3721 attccattgc tgatttccca gtgacaacat aggcgtacgt aacgttatgt gttgtgggaa
     3781 actcgctgca ccaccgacac tctccgcagc gcctttctgg aacagggcca gccactcggc
     3841 accattcatc ggcggcatat taagttcaac gccactttgt tccggcaacg gcgggagcgt
     3901 tttactgtct gccgcccaaa tagcacgatc gagcgtcagc ttttgaccaa gcaaccagcg
     3961 gctattgaaa tggttatccg caccagcctg tccggttaat tcaaagctgt tgagattgcc
     4021 atcaacctta acgtttaccg ctagtggttc acccgcaggt ttggctaacg gtgaaggtaa
     4081 gtgactactc acattattca gatcgccgtt cagctctacg ttataggtcg cgccagcatg
     4141 ataaggcaga tcaatgccca ctttaccatc ccacgccacg ctgccactca atgcttcgtt
     4201 caccgcttca ggcagaacgc cggttttcgc cggttgccag ttaccgttga gattcaccgc
     4261 aacctggtag gcttttgccc cttctttggt ggaaaaatcc acgttcaacg gctgattaaa
     4321 ccagcttgct gtcagtggtt cactttgcag atcgccattg ataaagctga atttaccgct
     4381 caaatttttc aggatgctgt cgagtggttt gataaacaga ctgttattac gcagcgtcac
     4441 ttcacctttc gcggttacca gttcgccgtt cagcgggata tcaagatgta agcgagcatt
     4501 cacatcgccg tcgagctgga gttcttgcag ggtcgcaccc agagaatctt tcagcggtgt
     4561 ctcatcaaag taagggccaa cggctttacc cggaccttta atgtcagcgt caatcagcag
     4621 tttttctttt gagtagtcag ggatcactgc ggtgagatta ctcgcgcgca cgccgcccag
     4681 attaacgcca tcggttttca tccataaacc gtcgttaata aagtccagtt caatatcaag
     4741 gttagttaat gcaggccagt ccggctggaa ggcaaacttc gcgttgcgca gcggcaccag
     4801 cacttcaaac tgaccttcgt tgtgtttata ggggaagagt tgcggattgc caccataaac
     4861 cagcgtcgcg ttatccgctt caccgccctg aatcgcacca cttaaataat caaccaggtc
     4921 tttacccatc aagttttccg ggaaatagcg ccaggcttgt gaaccatcat cggtactgat
     4981 gccagccaga atacccagcc agggttcatc gttagcaggt tgcaggtaac gaaaaccgcc
     5041 gcgcgcatgg acggctttgg ctttaacgtc aatattacgc ccatccagct ggaaaccttt
     5101 gtcattgttc agccagctta tagttgcctg gccgtcggcg atttccagtg gcgcacggaa
     5161 taccgtttcg taaggcatct ttgcctgctt catcgacgcg gtaagcaaac cattttcaac
     5221 gctgccggaa agcgtcccgg agaagtgttc cgcacccggt aataatttcc attgcttcca
     5281 ggccagatcg ctccacgatg cctgaaaacg ggtcttgtct gccgcctgaa gcgggatatc
     5341 cagcgccaga gtgttaatct tgccgctcgg ttgtgtggag cgccaaacat cacccagtgc
     5401 aggtgaaagt ttcgcggcca gcgggcgtat gccctccagg cctgccagct ccagattact
     5461 agcgcgaatc cggagttcgt cactgcgttt attgtctttg ccgccaacgt cctgttccgg
     5521 tatccaggcc agcgtcaatg ctccgctcgg ccagggtttg ccgtccatcg tgatccgtgt
     5581 atcgggaata gagaactgcc agcccggatt ttcacgcgta atatgcgcgg tcagattatc
     5641 caccgacagc gtatgcgttt gcttctcgcc caaccagctg gcaccgccct gtttcagcca
     5701 gacgtcaccg ccggttacat cgcctttgtc gatcgtcatc cagccttcaa gggagaactg
     5761 tgccgtttcc agagcaatat tgtcctgcat ccatttaccg agccacggct tcaggtcgat
     5821 gtcatccgcc tgtagccaga cgcgaccatt gcttaacaac ccctcatcat cgcgcaaatc
     5881 catgcgcacc tgcatcacgc cgtgctgtcc ggtaaggctg gagaggctta ccaggccttc
     5941 cgcacggtgt cgacgtggat cgttcagcca ggtgagttgt gggatcgcca gctcggcgcg
     6001 ctgaccggat ggcgtcagaa aactgacttc actgtcgcgg agatcgaaat ggtcaaattg
     6061 acgaagaaac agatcgctga tgtgactggc ttccagactg tcatcgctac caccgctggt
     6121 gataggagtg ttggtgcgaa agcgcagctg ccagaaagtg aggtcgcgaa actgccagcg
     6181 catatgtaac aggctctgcc agacatccag cgccagagta acgcgtttaa ccgaaaattc
     6241 gccgccatct tttagttctg cacggatgtc gtgtgcttca agcgtcgggc caaaattctg
     6301 ccagctggct gagagctgac tggcttctac cggcatgcca gtcgcggatt ctattttgtt
     6361 gaggatttcc ggacgccagg cgttaagatg cggtaaagca atacgcaggc cgctaaccag
     6421 cagggcagcg atcacaacga gcgcggctcc agtaagcagt aaaatccccg gcaatcgcct
     6481 cacccgtcac tccttgtctg ctaaaaatgt gactcaaaaa ccctttgccg gatggcggcc
     6541 cagcatctgt ttacatcatt acgacgtcaa actgctcctg gttatagagc ggttcaattt
     6601 gtactttaac ctgtttgcca acgaaaattt ccacttccgc cagcgagtgt gactcttcgc
     6661 ctttcaaggc ttcggctact gccggagaag catagaccag gaaacggtcg gagtcgtaag
     6721 catggtggac acgaacaatc tcgcgcatga tttcatagca taccgtttcc acggttttca
     6781 ccgttccgcg accgtggcag gttgggcatt cgttacacag tacgtgctca atgctttcgc
     6841 gggtgcgttt acgcgtcatc tccaccagcc ccagcgccga aaaaccatta acgctggttt
     6901 tcacccggtc tttgctcaac gcctgctcca gcgaatgcag cacgcggcgg cggtgatctt
     6961 cattattcat atcgatgaaa tcaataatga taatcccgcc cagattacgc aaccgtaact
     7021 ggcgagcgat agcctgcgtc gcttcaatat tggtattgaa aatagtgtcg tccagattgc
     7081 gatgaccgac aaacgctccg gtattgatgt ccacggtggt catcgcttcg gtctggtcga
     7141 taatcagata accaccggat ttcagttcta ctttgcgttc caacgctcgc tggatttcgt
     7201 tttcgacatc aaagagatcg aaaatcggct ggcgtccggt gtaatgctcc agcttgctgg
     7261 tcatctcggg aatgtactcc gaggtaaact ccagcaatgc ttcgtaagtc aggcgtgagt
     7321 caacgcgaat gcggtccagt tcggcgtcgg caaaatcacg cagaacacgc tgcgccagcg
     7381 ccagttcgcc gtacagctga taacgggtct gcgggcgctt tttacgctcc ataactttgg
     7441 tccagacgcg tttcagataa gcggcatcgg aggccagttc agcctcgcca accccttccg
     7501 ctgcggtgcg gatgataaac ccgccctgct cgtcgcaata ctctgcgacc acttttttca
     7561 gacgttcacg ttctgattcg ctttcaattc gttgggaaac cccaacgtga gaagcccctg
     7621 gcataaatac cagatagcga gaaggcagcg tgatatcggt ggtcaggcgc gcacctttag
     7681 tgccaagcgg atctttcacc acctgcacca tcagatcttg cccctgacga accagttccg
     7741 agatgtcgcg caccgtgaat tgcttttgtt cttcacccgc cacacattcg gtatgcggca
     7801 tgatgtcgga tgcatgaaga aacgcggctt tatccagccc aatatctaca aaagccgcct
     7861 gcatacccgg aagtacacga cttacacgac ccttgtagat attgcctact attccgcgtc
     7921 gcgcctcacg ttcaatatga atttcctgca gaataccgcc atcaatatac gccactcgcg
     7981 tttccgaagg cgttacgttt actaacaatt cagccgtcat gtttatccct tttctcacgc
     8041 agtgcgttaa aattacttaa taattcatac gtttcaacca gcggtaagcc gactacggcg
     8101 tgatagctgc catttatctt cctgacaaaa cagccaccca gcccctgaat accgtatgca
     8161 cctgctttat ctaacggttc accgctggcg acatagcccg cgatgtcttc gtctgttaac
     8221 gttctgaaag tcacatcggt gaccaccagg caatcgagaa tgtgctggct gtcggccaac
     8281 gccactgccg tcatcacctg atgggtctga cccgataatt tgcgcaacat ctgcgccgca
     8341 tgctctgcgt cgcgcggttt ctcgagcact tctccgttca ggataacgat aatatccgca
     8401 cccagcaccg ggagatcctg tgccgtttgc gcgacacccg cctgtgcttt ctcgcgtgcc
     8461 agacgcacaa catactgctg cgcgctctcc tgcggctgac gctgctcctc aatgcccgta
     8521 acaatacgtt caaaggtcac gccaagttgc gcaagtaact cctgacgacg cggagaaccg
     8581 gaagctaaat acagagaagt catagaaacc tttattgcac tgcaaactgc tgacggactt
     8641 tacgcatcag caagaaaatc cacggccaga gcaccccatt gactacacta ctccagaaca
     8701 cttccggtct gaaagagacg ttaatcacta aaaactctgc ccagaaaaca ataatatcca
     8761 ccaccagcga aagcaacatg acgaccagcg cctgctgcca taatgcgagg ttgcggaaaa
     8821 gctggtattt cagcgccacc aggtaagcaa tgatgctcat cgccaatacg cgtacgccaa
     8881 gcatcgagcc gctgatcaga tccagtatgg cacccatcac aaaacctgtg cccacattta
     8941 cgcgatgggg caaggccagg atccaataca acaagataag taacacccag tttggccgga
     9001 aaacaatcag gttatccggc cagggcatga tttgcagcaa cagcgcaatg aggaaagaga
     9061 gccagattac ccagcgtccc tggctacgat agctcgccac tattgccctc ccggcgcacg
     9121 cgcaggcggt tgagcaccac tttgcggcgg cgtagccctt tgtggagagc gattagcagc
     9181 gggctgtgtc ggcgcaacag gcgcagtagc tgcatttcct gtcgccggtt gctgcggagt
     9241 cggctgagcg atccccgttg ccggttcagg taactttggc cccatcgcgt ctggcgaagg
     9301 caatacctgc ggcatcatct gcatcagacg ttcattagca acacgatgca cctcttccgg
     9361 cgtcatcggg ttagcgccgt tacgatccgc gccccacagc agcagcagat aacgcaaacg
     9421 ttgcagtcct gcagtcggac gcgcctggat cacagtataa gcgcgctggg tatcgagttt
     9481 tacggaagag acaaccgcga ccggatagcc ttccgggaaa cgaccgccca gaccggaggt
     9541 caccagcaca tcaccaacac gaatatccgt attcgctggc agatgctcaa gctgcaaatc
     9601 atccgtacaa ccgttaccgg ctgcaattac gcggatatcg ttgcgcagca cctggattgg
     9661 cagcgcgtgg gtcgcatcac aaatcagcag cacgcgactg gtcagtttag cgacggccac
     9721 cacctgacca acaacacctt tgtcgctgat gaccggctgg ccttcataaa cgccattaac
     9781 gctaccttta tcgataacaa cttgatcgct ataaggatcg ttaaccgtgg agataacctg
     9841 agtcaccatt ttctgctcat cctgacgcag cggggaaccc agcagctcgc gcagacgcgc
     9901 gttctcctgt ttgtattgtc caagcatcag cagttcactg tttttcagca acagttcctg
     9961 acgtaacgcc cggttttcaa gttctagttg gtcacgcgag gccagcgtct gcgatatgcc
    10021 atccagcaat tcacgaggag cattggaaac aaagtagaaa ggactgacgg cggtatccat
    10081 ataagtacgg atttgactga acgtccccag gcggctgtcg gcaataataa tgccgagcgc
    10141 caccagcacc gccagaataa ggcgaatctg tagcgacggg ccacggctaa aaattggctt
    10201 cataagttat gcgtattctc gtatcagacc aggcagggta aacagacgct tcccctgcct
    10261 gcatccgatt actcttcgct gaacaggtcg ccgccgtgca tgtcgatcat ttccagcgct
    10321 ttgccgccac cgcgcgccac acaggtcagc gggtcttcag caacaacgac tggaatgccg
    10381 gtttcttcca ttaacaaacg gtcaaggtta cgcagcagtg cgccaccacc ggtgagcacc
    10441 atgccgcgct cggagatgtc ggaagccagt tccggcgggc actgttccag tgcaaccatt
    10501 accgcgctca caataccggt cagcggttcc tgcagtgctt cgaggatttc gttggagttc
    10561 agggtaaaac cgcgtggaac accttctgcc aggttacggc cacgaacttc gatttcacgg
    10621 acttcatcgc ccgggtaagc cgaaccgatt tcgtgcttga tacgttctgc ggtggcttca
    10681 ccgatcagag aaccgtaatt acgacgcaca tagttgatga tagcttcgtc gaaacgatca
    10741 ccaccaatgc gcacggaaga ggagtaaacc acgccgttca aggagataac agcaacttca
    10801 gtggtaccac caccgatatc aaccaccata gaaccggtcg cttcagaaac cggcaggcca
    10861 gcaccaattg cggcagccat cggttcttca atcaggaaga cttcacgggc accagcgccc
    10921 tgcgcggatt cacgaattgc gcggcgttca acctgggtcg cgccaaccgg tacacaaacc
    10981 agaacgcgcg ggcttggacg cataaagctg ttgctgtgca cttgtttgat gaagtgctgg
    11041 agcatttttt cagtcacgaa gaagtcggcg ataacgccgt ctttcattgg gcgaatggca
    11101 gcaatattgc ccggcgtacg gcccagcatc tgcttcgcgt catgacctac tgcagctacg
    11161 cttttcggtg aaccggcacg atcctgacga atggccacca cggaaggctc attcaatacg
    11221 atgccttgtc cttttacata aatgagggta ttcgcagtac ccaggtcaat ggacaagtca
    11281 ttggaaaaca tgccacgaaa ttttttcaac atactaaggg ataatcctga aagctggggc
    11341 ggaaaagaaa atccgcttac tttaccaacc acacgcagca gcgacaaggc gcaaaaatca
    11401 tctgctacgg tgaaaattag tgcagttcgt ttcctttgtt acaaatctct gcctgagtcc
    11461 agaaaggctt aatgcatcag cagcattcct cgcctgtttg caaccgcgta aggtcattca
    11521 tctgcatatg tgctgcaaca atctggcgag cagacaagca cactcccatg agacgcagcg
    11581 cgcgttattc tacgtgaaaa cggattaaac ggcaggttaa accgagtatc tttgtgaata
    11641 ttttttcacg ttagtatcaa gtggctgtga ggacgcgaaa aaatcccctt gcccgcctgt
    11701 aacaccgcgc tgaatcaggg tctgccactc gcttcgcgaa cgcacgccgg tggcgtaaac
    11761 gtgggtgctg gtcccggagc aggcttccac caggctttga accagcagct ggttctccgt
    11821 tcgcttctca atgttcctga ccagccccgg atggagcttg agtaactcaa cattaagttc
    11881 tttgatccaa ctggtgctta ccagcgtcaa accagcctgg ttgacggcta cccgtacccc
    11941 taaagcattc actaaacgaa taacaggttg taaacgactg atatgttgac ctacatcggc
    12001 ctctgcaagt tcaataatta tgcgttttcg ttgtgatttt tcacattgca ttaacgtatc
    12061 gcgcagccaa cgctgaaaac gcggacgaat cagcgactca acggtaacct gaatcgccag
    12121 attttcctct ggccagtaac gcaatagtgg aataagacgg ctgatttgca gacggtcata
    12181 ctcttccgat aagccaaact gcaagaccat cggcatatac tccgccgagc taacctcttc
    12241 attaccatcg aagatgcggc acatgagttc gcgatgatga acctgacctt cgcgagtaac
    12301 cgccggtttt tgataaaggc gcgggccgcc gcgactgagc atttgctcga taagcgtacg
    12361 ccagcgaaca ttaccgcgtc ctttttcagg caacgagtca tcataaatag cccagctatt
    12421 gccaccctgc aatcccgcat tacgcgtggc agactctgca tgttccatta cctgctcggt
    12481 atcctgacca ctacgccagg cacagatacc aatgtggatc atatcgtcgc gatcgagcat
    12541 tttattgttc ggcaaggtat cgacggcttt gattaactga ctggctatgc tctctgcctc
    12601 ttttaacgtc cggtgcggta acagcgcagc aaaatcactg cggtggtaac gcgccagcag
    12661 tgcgccaggg tagcgcatca taaatgtcga cagcagattc gtcagagtga agaactgttc
    12721 ttcaacctgg ctgtgccccc aggtatcgct caacatattg aaatccggca gacgaatcat
    12781 catcacgatc ccgtgggtac ctactttctc ctgatcttcc agtaacgttg ctaactgatt
    12841 atcgaaaaag agtcggttat tgaggccggt tttcacgtcc tgtgcggcat aagagcggat
    12901 cagcgtatca agacggctgt gttgttcgcg tgcgttctga atttcacgaa gcagcgtatc
    12961 cagcgcactg ctggttctgg gcggccattc atagatggtt cccaacacat tagagccacg
    13021 ctcaccgttt aagatacgag tagcccgggt ttccagcaat tcttgcccgg caagttgccg
    13081 ttgtaaccag cgtaccgcca gaaagagcat aagaatgata aagccaatcg cccccgtgag
    13141 cggcgcggtg gtcatcaacg aatggaaata gttgcccatc ggatcctgat aaaccagacg
    13201 caacgacatc cccggatgct ttatcaacgg aacgctcagt tcacgaaaca gatcgctggt
    13261 gccaaccgga cgataactac catttctggc cagggtataa acctgtttat caccatgcag
    13321 caggtctaca cgaacgatat ctgctgacac cattaattcg gtaatttgtg gccttaatgc
    13381 gctgaagtca ttcgacacaa ggtgggtatc aatcgccgtc gccaccgcct gaacgcgatg
    13441 actaaactta tactgaatgg cgttgtagaa acttagcgaa cagcccagca aagtcacaaa
    13501 aattgttaac ccggtgagca gcgtaacaaa ggccgaaaat ttcgtcgtta atctcatcct
    13561 tgtgttaact ccgatagtga ggaagcgggc atactagcaa atcagattta tctcgcaatt
    13621 tattgcgctt catcggcttt gcttttccat tagcgagtat agtcttcaga aattattttc
    13681 caatccatca tgcacatgag gaccacttat gcaggcgtta cttttagaac agcaggacgg
    13741 caaaactctc gcatcagtac agactctgga cgaacgtcgc ctgccggagg gcgatgtcac
    13801 ggtcgatgtt cactggtcga gcctgaacta taaagatgcg ctggcgatta ccggtaaggg
    13861 aaaaatcatc cgtaattttc cgatgattcc tgggatcgat tttgccggaa ctgtacgcac
    13921 cagcgaagat ccgcgttttc atgccggtca ggaggtgtta ctcactggct ggggcgttgg
    13981 tgaaaaccac tggggtgggc tggcggagca ggcgcgagtg aaaggtgact ggctggttgc
    14041 catgccgcaa gggctggacg cgcgtaaagc aatgattatc ggtactgccg gttttaccgc
    14101 catgctgtgt gtgatggcgc tggaagatgc cggtgttcgc ccgcaggacg gtgagattgt
    14161 cgtgacgggt gccagtggtg gcgtcggcag taccgccgtg gcgctgctgc ataagttggg
    14221 ttatcaggtc gttgccgttt ccggtcgcga aagtacccat gaatatctga aaagtttagg
    14281 tgccagcggt attctccctc gtgatgagtt tgccgaatcc cgtcctctgg aaaaacaagt
    14341 ctgggctggg gcaattgaca ctgttggcga caaagtgctg gcaaaagtgc tggcgcaaat
    14401 gaattacggc ggctgcgtgg cggcctgtgg tctggcgggt ggttttactc tgccaaccac
    14461 ggtcatgcca tttattctgc gtaatgtccg tttgcaaggg gtggattcag taatgacgcc
    14521 accagaacgc cgcgcacaag cctggcagcg actggtcgcc gatttaccgg aatcattcta
    14581 tacccaggcg gcaaaagaga tatctctgtc agaggcaccg aactttgccg aggccatcat
    14641 taataaccag atccagggtc gcacgctggt gaaggttaac taaccattta gcaaggaata
    14701 ataagagagg gaactcattt tgacatcatt attcaatcgg ttaacgggaa aagcggttag
    14761 ccggacagct ttcgtcgaac accttggtca ggaagttgta caacatcatc caaactggaa
    14821 agtcatgatt tcgactgacc acaaattgat gcgcattgat actcctctaa acagccatta
    14881 ttgatacgcc tccgtcgcct gttaggttta tggtgctttg cctgggcgac actacgctta
    14941 acctgttact gatttctggt accatggggt gaataatctg attttatttg actacaaatt
    15001 aaccactcga acctatttaa tgctgagaat tgtcagttgg ttgattttgc gtgctttagc
    15061 attcacatct atccagacga tgcagtgaaa attgggtaat ccccagcaac cgctgcgtaa
    15121 tgtcgtctat cttgtcgcga tcctggcatc cctacattat ttgtggtctg tgaagattat
    15181 ctcattgcag cccctcatct tcgcagggct ggcttttcag cttttcacct tacgttataa
    15241 gaagttccgt cgatgatggc gctaatttcg tgaattgtgc ggcttgttgc aaattacacg
    15301 gtgttgaagg ttatttacat gttagctgtt gattatattc cctgataaga ccagtattta
    15361 gctgccaatt gctacgaaat cgttataatg tgcgacctcg tcctccctga cgcagttttt
    15421 gcgctgcgga aaaggtgaca ttggcgcaac gaaggtatat tttgtttttt gccggaggat
    15481 agcagcagat cgctgcacaa tgtccgtcaa gtctaacatt gacactctgg ggcaaaatag
    15541 accggcgtcc cggcctgctg gaatttatcg ctatgcatac agctgtcggg gcatacgctt
    15601 tacagacggc ggtgaaacgc ctgtcacaat cacactaaac aaagagtacg gaacccactc
    15661 atggatattc gtaagattaa aaaactgatc gagctggttg aagaatcagg catctccgaa
    15721 ctggaaattt ctgaaggcga agagtcagta cgcattagcc gtgcagctcc tgccgcaagt
    15781 ttccctgtga tgcaacaagc ttacgctgca ccaatgatgc agcagccagc taaatctaac
    15841 gcagccgctc cggcgaccgt tccttccatg gaagcgccag cagcagcgga aatcagtggt
    15901 cacatcgtac gttccccgat ggttggtact ttctaccgca ccccaagccc ggacgcaaaa
    15961 gcgttcatcg aagtgggtca gaaagtcaac gtgggcgata ccctgtgcat cgttgaagcc
    16021 atgaaaatga tgaaccagat cgaagcggac aaatccggta ccgtgaaagc aattctggtc
    16081 gaaagtggac aaccggtaga atttgacgag ccgctggtcg tcatcgagta acgaggcgaa
    16141 catgctggat aaaattgtta ttgccaaccg cggcgagatt gcattgcgta ttcttcgtgc
    16201 ctgtaaagaa ctgggcatca agactgtcgc tgtgcactcc agcacggatc gcgatctaaa
    16261 acacgtatta ctggcagatg aaacggtctg tattggccct gctccgtcag taaaaagtta
    16321 tctgaacatc ccggcaatca tcagcgccgc tgaaatcacc ggcgcagtag caatccatcc
    16381 gggttacggc ttcctctccg agaacgccaa ctttgccgag caggttgaac gctccggctt
    16441 tatcttcatc ggcccgaaag cagaaaccat tcgcctgatg ggcgacaaag tatccgcaat
    16501 cgcggcgatg aaaaaagcgg gcgttccttg cgtacctggt tctgacggcc cgctgggcga
    16561 cgatatggat aaaaaccgcg ccattgctaa acgcattggt tatccggtga ttatcaaagc
    16621 ctccggcggc ggcggcggtc gcggtatgcg cgtagtgcgc ggcgacgctg aactggcaca
    16681 atccatctcc atgacccgtg cggaagcgaa agctgctttc agcaacgata tggtttacat
    16741 ggagaaatac ctggagaatc ctcgccacgt cgagattcag gtactggctg acggtcaggg
    16801 caacgctatc tatctggcgg aacgtgactg ctccatgcag cgccgccacc agaaagtggt
    16861 cgaagaagca ccagcaccgg gcattacccc ggaactgcgt cgctacatcg gcgaacgttg
    16921 cgctaaagcg tgtgttgata tcggctatcg cggtgcaggt actttcgagt tcctgttcga
    16981 aaacggcgag ttctatttca tcgaaatgaa cacccgtatt caggtagaac acccggttac
    17041 agaaatgatc accggcgttg acctgatcaa agaacagctg cgtatcgctg ccggtcaacc
    17101 gctgtcgatc aagcaagaag aagttcacgt tcgcggccat gcggtggaat gtcgtatcaa
    17161 cgccgaagat ccgaacacct tcctgccaag cccaggcaaa atcacccgtt tccacgcacc
    17221 tggcggtttt ggcgtacgtt gggagtctca tatctacgcg ggctacaccg taccgccgta
    17281 ctatgactca atgatcggta agctgatttg ctacggtgaa aaccgtgacg tggcgattgc
    17341 ccgcatgaag aatgcgctgc aggagctgat catcgacggt atcaaaacca acgttgatct
    17401 gcagatccgc atcatgaatg acgagaactt ccagcatggt ggcactaaca tccactatct
    17461 ggagaaaaaa ctcggtcttc aggaaaaata agactgcaaa agcgtcaaaa ggccggattt
    17521 tccggccttt tttattactg gggatcgaca acccccataa ggtacaatcc tcgctttctt
    17581 cacccatcag ggacaaaaaa tggacactcg ttttgttcag gcccataaag aggcccgctg
    17641 ggcgctgggg ctgacccttt tgtatctggc agtttggtta gtagacgctt acttacctgg
    17701 cgttgccccc ggttttaccg gctttccgcg ctggtttgag atggcctgca tcctgacgcc
    17761 gctactgttt attggactgt gctgggcgat ggtgaaattt atctatcgcg atatcccact
    17821 ggaggatgac gatgcagctt gaagtaattc taccgctggt cgcctatctg gtggtggtgt
    17881 tcggtatctc ggtttatgcg atgcgtaaac ggagcaccgg caccttcctt aatgagtatt
    17941 tcctcggcag ccgctctatg ggcggtattg tgctggcgat gactctcacc gcgacctata
    18001 tcagtgccag ttcgtttatc ggcgggccag gagctgctta taaatacggg ctgggctggg
    18061 tattgctggc gatgattcag cttcctgctg tctggctttc actcggtatt ctcggcaaga
    18121 agtttgcgat tcttgcgcgc cgctacaatg cagtgacgct gaacgatatg ctgtttgccc
    18181 gctaccagag tcgtcttctg gtgtggctgg cgagtttgag tttgctggtt gcgttcgttg
    18241 gtgcgatgac cgtgcagttt atcggcggtg cgcgcctgct ggaaaccgcg gcgggtattc
    18301 cttatgaaac cgggctgctg atttttggta tcagcattgc gttatatacc gcctttggtg
    18361 gctttcgcgc cagcgtgctg aacgacacca tgcaagggct tgtgatgctg attggcaccg
    18421 ttgtgctgct tattggcgtg gtacatgccg ctggcggctt aagcaacgca gtacagacct
    18481 tgcaaaccat cgatccgcaa ctggttacgc cacaaggcgc tgacgatatt ctgtcgcctg
    18541 cctttatgac gtcgttctgg gtactggtgt gctttggcgt gattggcctg ccgcatactg
    18601 cggtgcgctg tatctcttat aaagacagca aagcagtaca ccgggggatc atcatcggta
    18661 cgattatagt cgcaatcctg atgttcggta tgcacctggc cggagcgtta ggtcgtgcag
    18721 tgatccccga tctcaccgta ccagatctgg tgatcccgac gttaatggta aaagtgctgc
    18781 caccttttgc tgccgggatc tttctggctg cacctatggc tgcgatcatg tcgacaatta
    18841 acgcccaact gctgcaaagt tccgctacga tcattaaaga tctctatctg aatatccgtc
    18901 cggatcaaat gcaaaacgag acgcgtctga agcggatgtc ggcggtaatt acgttagttc
    18961 tcggcgcgtt gctgctgctt gccgcctgga agccgccaga aatgatcatc tggctgaatt
    19021 tgctggcctt cggtgggctg gaagccgttt tcctgtggcc gctggtgctg ggtctttact
    19081 gggaacgcgc caacgccaaa ggcgcgctaa gtgcgatgat cgttggcggc gtgctgtatg
    19141 ccgtactcgc gacgctgaat attcagtacc tgggcttcca ccctatcgtg ccctcgttac
    19201 tactaagttt gctggctttc ctggtcggaa accgtttcgg tacatccgtc ccgcaagcta
    19261 ccgttttgac tactgataaa taaagagttt tgccatgcct tggatccaac tgaaactgaa
    19321 caccaccggc gcgaacgcgg aagatcttag cgatgcgctg atggaagcgg gtgccgtttc
    19381 tatcactttt caggataccc acgatacgcc agtatttgag ccgctgccgg gcgaaacgcg
    19441 cctgtggggc gacaccgatg tgattggtct gttcgacgct gaaaccgata tgaacgacgt
    19501 ggtggcgatt ctggaaaacc atccgctgct cggcgcaggc ttcgcgcata aaatcgaaca
    19561 actagaagat aaagactggg agcgcgaatg gatggataat ttccacccga tgcgctttgg
    19621 tgaacgactg tggatctgcc ctagctggcg tgatgtgccg gacgaaaacg ccgtcaacgt
    19681 gatgttagat ccagggctgg cgtttggtac gggtacccat ccaaccacct ctctgtgcct
    19741 gcaatggctc gacagcctcg atttaaccgg taaaacagtc atcgactttg gctgtggttc
    19801 cggcattctg gcgatcgcgg cgctgaaact gggtgcagca aaagccattg gtattgatat
    19861 cgatccgcag gcgattcagg ccagccgcga taacgccgaa cgtaatggcg tttctgaccg
    19921 tctggaactc tacttaccga aagatcagcc agaagaaatg aaagccgacg tggtggtcgc
    19981 taacatcctt gcaggcccat tacgtgaact ggcaccgtta atcagcgtcc tgccagtttc
    20041 aggcggtttg ctgggccttt ccggtattct ggcaagccag gcagagagcg tttgtgaagc
    20101 ttatgccgat agcttcgcac tggacccggt cgtggaaaaa gaagagtggt gccgtattac
    20161 cggtcgtaag aattgacctt cgcatcgccg tagggtgacg cggggcgaag tgcgagcaag
    20221 ctcacaaaag gcacgtaaat ttgccgatta tttacgcaaa tttgcgtgcc aaaattttca
    20281 ttcataaaga aaaattgaga acttactcaa atttctttga gtgtaaattt tagtcactat
    20341 tttctaatat gatgattttt atgagtaatt atcgcaccac gctcatttta aatgcaattc
    20401 tttgatccat ctcagaggat tggtcaaagt ttggcctttc atctcgtgca aaaaatgcgt
    20461 aatatacgcc gccttgcagt cacagtatgg tcatttctta actcatgcgc atcggacaat
    20521 atcagctcag aaatcgcctg atcgcagcgc ccatggctgg cattacagac agaccttttc
    20581 ggacgttgtg ctacgagatg ggagccggat tgacagtatc cgagatgatg tcttctaacc
    20641 cacaggtttg ggaaagcgac aaatctcgtt tacggatggt gcacattgat gaacccggta
    20701 ttcgcaccgt gcaaattgct ggtagcgctc cgaaagaaat ggcagatgca gcacgtatta
    20761 acgtggaaag cggtgcccag attattgata tcaatatggg ttgcccggct aaaaaagtga
    20821 atcgcaagct cgcaggttca gccctcttgc agtacccgga tgtcgttaaa tcgatcctta
    20881 ccgaggtcgt caatgcagtg gacgttcctg ttaccctgaa gattcgcacc ggctgggcac
    20941 cggaacaccg taactgcgaa gagattgccc aactggctga agactgtggc attcaggctc
    21001 tgaccattca tggccgtaca cgcgcctgtt tgttcaatgg agaagctgag tacgacagta
    21061 ttcgggcagt taagcagaaa gtttccattc cggttatcgc gaatggcgac attactgacc
    21121 cgcttaaagc cagagctgtg ctcgactata caggggcgga tgccctgatg ataggccgcg
    21181 cagctcaggg aagaccctgg atctttcggg aaatccagca ttatctggac actggggagt
    21241 tgctgccccc actgcctttg gcagaggtta agcgcttgct ttgcgcgcac gttcgggaac
    21301 tgcatgactt ttatggtccg gcaaaagggt accgaattgc acgtaaacac gtttcctggt
    21361 atctccagga acacgctcca aatgaccagt ttcggcgcac attcaacgcc attgaggatg
    21421 ccagcgaaca gctggaggcg ttggaggcat acttcgaaaa ttttgcgtaa acagaaataa
    21481 agagctgaca gaactatgtt cgaacaacgc gtaaattctg acgtactgac cgtttctacc
    21541 gttaactctc aggatcaggt aacccaaaaa cccctgcgtg actcggttaa acaggcactg
    21601 aagaactatt ttgctcaact gaatggtcag gatgtgaatg acctctatga gctggtactg
    21661 gctgaagtag aacagcccct gttggacatg gtgatgcaat acacccgtgg taaccagacc
    21721 cgtgctgcgc tgatgatggg catcaaccgt ggtacgctgc gtaaaaaatt gaaaaaatac
    21781 ggcatgaact aattcaggtt agctaaatgc ttgattaaaa aggcgctact cggcatgggg
    21841 aagcgccttt tttataggtg tcacaaaggg agtgaccatg agaacaggat gtgaaccgac
    21901 ccggtttggt aatgaagcta agaccattat tcacggtgat gcctttgccg aacttaaaaa
    21961 gctacctact gaaagcgtcg atctgctctt tgccgaccca ccgtataaca tcggtaaaaa
    22021 ttttgatggt ctgatcgaag cctggaaaga agatctgttt atcgactggc tgtttgaagt
    22081 gattgcagag tgccaccgcg ttctgaaaaa gcagggcagc atgtacatta tgaacagtac
    22141 ggaaaacatg ccctttatcg atctccagtg ccgcaagctt tttaccatca aaagtcgcat
    22201 cgtctggtca tatgacagtt ctggagtaca ggcgaaaaaa cactacggct ccatgtacga
    22261 acccatcctg atgatggtga aagacgcaaa gaactacaca ttcaacggtg atgctattct
    22321 ggtagaagcc aaaaccggat cgcagcgcgc gttgatcgat tatcgcaaaa atcctccaca
    22381 gccatacaat catcaaaaag taccgggtaa cgtctgggat tttccgcgcg tgcgttattt
    22441 gatggatgaa tatgagaacc acccgacgca aaaaccgaaa gccttactga aacgcattat
    22501 tctcgcctct tccaacccag gcgatatcgt tctcgacccg tttgccggta gctttactac
    22561 cggtgccgta gccatcgcca gcggacgaaa attcattggt attgagatca acagcgagta
    22621 catcaaaatg gggcttcgac ggctggatgt cgcgtcgcat tactccgcgg aagaactggc
    22681 gaaagtgaaa aaaagaaaga ccggcaacct gtcaaaacga agccggttaa gcgaagttga
    22741 ccccgatctc attgcaaagt aaaggaagtg taagcctagt tttcagatta ttcatttcgt
    22801 gtatatttcc tgccagactt ggttaaacat gcacaggctc aggtaatgat tcgcaagtat
    22861 tggtggctcg tcgttttcgc tgtcttcgtt tttctgtttg atactttact gatgcagtgg
    22921 attgaactgc tgacaacaga aacagacaaa tgccgtaata tgaactcagt taatccacta
    22981 aaactggtta actgtgacga actgaatttt caggacagaa t
//
LOCUS       DAZPCS010000047        22943 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZPCS010000047 DAZPCS010000000
VERSION     DAZPCS010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22943
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.047"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            59..613
                     /locus_tag="ACHUQ7_001854"
     CDS             59..613
                     /locus_tag="ACHUQ7_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR1135289.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTMLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(623..2050)
                     /locus_tag="ACHUQ7_001855"
     CDS             complement(623..2050)
                     /locus_tag="ACHUQ7_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFR1135290.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVYYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(2047..2754)
                     /locus_tag="ACHUQ7_001856"
     CDS             complement(2047..2754)
                     /locus_tag="ACHUQ7_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR1135291.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRIIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            2918..3895
                     /gene="ybeQ"
                     /locus_tag="ACHUQ7_001857"
     CDS             2918..3895
                     /gene="ybeQ"
                     /locus_tag="ACHUQ7_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
                     /protein_id="HFR1135292.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFWYK
                     QAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEALL"
     gene            complement(3965..4447)
                     /locus_tag="ACHUQ7_001858"
     CDS             complement(3965..4447)
                     /locus_tag="ACHUQ7_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFR1135293.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTEIDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EIFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            4682..7264
                     /gene="leuS"
                     /locus_tag="ACHUQ7_001859"
     CDS             4682..7264
                     /gene="leuS"
                     /locus_tag="ACHUQ7_001859"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFR1135294.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALQAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            7279..7860
                     /gene="lptE"
                     /locus_tag="ACHUQ7_001860"
     CDS             7279..7860
                     /gene="lptE"
                     /locus_tag="ACHUQ7_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFR1135295.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTMLGN"
     gene            7860..8891
                     /gene="holA"
                     /locus_tag="ACHUQ7_001861"
     CDS             7860..8891
                     /gene="holA"
                     /locus_tag="ACHUQ7_001861"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFR1135296.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            8893..9534
                     /gene="nadD"
                     /locus_tag="ACHUQ7_001862"
     CDS             8893..9534
                     /gene="nadD"
                     /locus_tag="ACHUQ7_001862"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFR1135297.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            9558..10169
                     /locus_tag="ACHUQ7_001863"
     CDS             9558..10169
                     /locus_tag="ACHUQ7_001863"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HFR1135298.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            10429..10746
                     /gene="rsfS"
                     /locus_tag="ACHUQ7_001864"
     CDS             10429..10746
                     /gene="rsfS"
                     /locus_tag="ACHUQ7_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFR1135299.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            10750..11217
                     /gene="rlmH"
                     /locus_tag="ACHUQ7_001865"
     CDS             10750..11217
                     /gene="rlmH"
                     /locus_tag="ACHUQ7_001865"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFR1135300.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            11248..13149
                     /gene="mrdA"
                     /locus_tag="ACHUQ7_001866"
     CDS             11248..13149
                     /gene="mrdA"
                     /locus_tag="ACHUQ7_001866"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFR1135301.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     EPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTANKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            13152..14264
                     /gene="mrdB"
                     /locus_tag="ACHUQ7_001867"
                     /gene_synonym="rodA"
     CDS             13152..14264
                     /gene="mrdB"
                     /locus_tag="ACHUQ7_001867"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFR1135302.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            14275..15363
                     /gene="rlpA"
                     /locus_tag="ACHUQ7_001868"
     CDS             14275..15363
                     /gene="rlpA"
                     /locus_tag="ACHUQ7_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFR1135303.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTASSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            15502..16713
                     /gene="dacA"
                     /locus_tag="ACHUQ7_001869"
     CDS             15502..16713
                     /gene="dacA"
                     /locus_tag="ACHUQ7_001869"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFR1135304.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFSDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            16824..17087
                     /gene="ybeD"
                     /locus_tag="ACHUQ7_001870"
     CDS             16824..17087
                     /gene="ybeD"
                     /locus_tag="ACHUQ7_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFR1135305.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            17188..17829
                     /gene="lipB"
                     /locus_tag="ACHUQ7_001871"
     CDS             17188..17829
                     /gene="lipB"
                     /locus_tag="ACHUQ7_001871"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFR1135306.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDNSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            18088..19041
                     /locus_tag="ACHUQ7_001872"
     CDS             18088..19041
                     /locus_tag="ACHUQ7_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HFR1135307.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFILRDPILHGVIDVIRNAF"
     gene            19250..20215
                     /gene="lipA"
                     /locus_tag="ACHUQ7_001873"
     CDS             19250..20215
                     /gene="lipA"
                     /locus_tag="ACHUQ7_001873"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFR1135308.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(20316..20519)
                     /gene="tatE"
                     /locus_tag="ACHUQ7_001874"
     CDS             complement(20316..20519)
                     /gene="tatE"
                     /locus_tag="ACHUQ7_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFR1135309.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(20648..21436)
                     /locus_tag="ACHUQ7_001875"
     CDS             complement(20648..21436)
                     /locus_tag="ACHUQ7_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFR1135310.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            21529..21912
                     /gene="crcB"
                     /locus_tag="ACHUQ7_001876"
     CDS             21529..21912
                     /gene="crcB"
                     /locus_tag="ACHUQ7_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFR1135311.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTVH"
     gene            complement(21966..22175)
                     /gene="cspE"
                     /locus_tag="ACHUQ7_001877"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(21966..22175)
                     /gene="cspE"
                     /locus_tag="ACHUQ7_001877"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFR1135312.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(22350..22910)
                     /gene="pagP"
                     /locus_tag="ACHUQ7_001878"
     CDS             complement(22350..22910)
                     /gene="pagP"
                     /locus_tag="ACHUQ7_001878"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFR1135313.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT         5909 a         5750 c         5668 g         5616 t
ORIGIN      
        1 tattgatgag tataaacttg acgctttcaa aataaaaaga aaatcgaagc attcacacat
       61 gaataaaaaa ttaatgtata tattcgcaat ttttatagtt gcagcaatta cctgtattag
      121 ccaacccaag aaaacgatgt tgcgtgataa agccatggtg aattatgcct ttgattattt
      181 aagatcacca ggcagtctac cgttaaccac ggcagccacg gagctttccg cgattcatgg
      241 tcactcaaca tcgcaatatc gccttggcga attttatctt catggtagcg acggtaaacc
      301 actggattat acacaggcga gatactggta tgagcaatca gcagaacagg aaaatccgcg
      361 cgcacaaagc aaactggggt ggatctacct gaaaggcctg ggagtcaaac ccgatacccg
      421 taaagcaatt ctctggtata aggaagcagc tgaacaaggg tatgctcatg ctcaatatac
      481 tttgggtttg atctacagaa atggcactgg tattaatgtt aaccattatg aatctcaaaa
      541 atggttaaaa ctggccgcca aacaacatta caaaaatgcg gaaagattac ttgccggact
      601 tcccgcacat taacgtcgac tgtcacccga agagatggag tattttccct atcaaaccgg
      661 caaagataaa aatgtagaaa ataattttga tgacgcccag acagcccagc ccgccatcat
      721 caacgggcat tggctgcggt tgcggctccg gctcgacggt ttcctgaccg gtaaaattcg
      781 ccgcctgaat taataatcga cgaataatta atgcttccag tggacaacga tcttctgcat
      841 caaggcggct cacaatcggc attaatgatt cttctgtcgg ttggctggcc tgcactaatt
      901 gtttggcaac taaatataat ggccataagg gatgttctgt atcacgcgtg agtgagatcc
      961 gcggcttacg tcctttgtgc aaaaccatct cgaccagtga acgccccagc gcagagagtt
     1021 tttcatcgct gagtatttcg tataaatgaa aacgcgtacg ggcgctgtcg ccggggacat
     1081 aaagtagcgg ttttccctga tcgttaacca gatcacgcgc ttcaatcatc atcaccacta
     1141 atgggtaata atagacccga tgctgacggc accattgcaa cattaaagca tccgcctggg
     1201 tggagggata ctcgcgccag tagtaacaaa gttctgccag caggctttcg ccttctccac
     1261 aatagacgcg ttgtgcgtat tcataataac gcgcatcttc attatcaggt tcagcttccc
     1321 gccacgaccg ggcgtaatcc aggagttcgg ggattcccag gcggcttgcg ctataccagc
     1381 ggagtaaatt tctgtgcagt tttgcatcat cggggataat cactgcgcga tgttgcgcta
     1441 aatacaatgt cagccattct ggatgcgatg accaaatccg agcgaacatt tggtaaaaat
     1501 cgatggtgtc attttgcacg gcaactggca gatgcagaat ggtctggaag ttaaaaacat
     1561 tccccttcgc aatggcatat aaaaaggatt ccagttcttc ttcgtctatt tccccgacgt
     1621 tttcctcctg caaccaccgc aatctttctg ctaataacac cacgcactcg aaggataaat
     1681 gggcagtgtt catggcgatt gtgcacagcg accagcgtaa ttcatcaatc tcttccatcg
     1741 agcaataatt taattgctga atgaatcgct gccaggcgga gggcagaaaa cgttcgctat
     1801 cagaggcaag taacgcgcga aaggcgagca gaatttcatg ttctgctacc tcatattctt
     1861 ctggttgcca aacagattta gcaggcgact gcgcaatccg tagcgcctcc tcatacgcct
     1921 gacgaagttg tttaaaaccc tgcggatcgg tttctggatg aaaggacggt aacagcgcca
     1981 gataagcgcg gcggataata tcgacgtcag tcgtttcctc tatatcgagg atcttccagc
     2041 aatttttcat aagtcgctca tatccttaag cgtttccggg catgtggggt agtcagccag
     2101 tggttgccag gacaaggtag ggtaatacgc atcatcactg gcaaataatt gataaaacat
     2161 atcattgccg gaatcgtcgt attctttacg cgctaagaat tcgcgtaata aaggcagatg
     2221 atcatcttcg ggagactgcc aatattgccg accaaaggta taaccggcaa agtattgccg
     2281 ccagttacta taataacgta gagcccgtag atgaatacgc gactggatcc atagactttc
     2341 ttcttcactc aaccagttat taagtacgcc cattcgactt aaaaaaccca tccggacata
     2401 atcccatgct ttaattcctc cctcaccaca gcattcggct gtgctggcga caaattgcgc
     2461 ataagcctga ccttgctcat ttaattcagc aagatagtca cgccattcac atggagaata
     2521 acgaaaccag cggtgataaa accctgccaa ccgagcggca tgaccgttct cgatcattcg
     2581 gtgaattatg cgcagcaggt catcacggtc atcaattccc catgaggaag aaaggtcaaa
     2641 aaagcatgta cctaaataca ttttggggga tgaatgcgaa ggtaaacagc attcattgcg
     2701 tatttccatc ggagtggaaa gtgcaaacaa tattttttgc gaccccatgt ccatataatt
     2761 tatccttaaa cattcattta atgaatgtaa actacatttc gaggctcagt tatttatact
     2821 tttctgacgc aacataatac gcataaaata aggcttacgc aaaaatggaa cacataaaaa
     2881 tataatatat tttatctaca accataagga aatgataatg atttttacgt caagttgctg
     2941 cgataattta tcaatagatg agattatcga acgtgctgaa aaaggcgatt gcgaggctca
     3001 atatattgtt gggttttatt ataatcgcga tagcgcaatt gactctccgg acgacgaaaa
     3061 ggccttttac tggctgaagc tggccgctga gcaaggtcat tgtgaagcac agtattcctt
     3121 agggcaaaag tataccgagg ataaaagccg tcataaagat aatgagcaag ccatcttctg
     3181 gctgaaaaaa gctgccctac aaggccatac tttcgcttcc aacgcccttg gctggatact
     3241 ggatcgtgga gaagacccca actataaaga agcggttacc tggtatcaga tagccgcgga
     3301 gagcggaatg tcttatgcgc aaaataatct tggatggatg tacagaaatg gcaacggagt
     3361 cgcacaagac tatacgctgg catttttctg gtacaaacaa gctgcattac aaggccatag
     3421 ttacgcacaa gacaatctgg ccgatcttta taaagacgga gaaggcgttg ctcaaaacaa
     3481 gacactcgcc gcattctggt atttgaaaag cgcacagcag ggtaatcggc acgcccagtt
     3541 tcaaattgca tgggattata acgctggcga aggggtggac caggactata agcaagcgat
     3601 gtactggtat ctgaaggctg ccgctcagga aagcgtcggc gcttacgtca acatcggtta
     3661 tatgtataaa cacggacaag gcgttgagaa agattatcag gctgcctttg aatggtttac
     3721 gaaagccgct gaatgcaatg acgccactgc ctggtataac ctggccatta tgtatcacta
     3781 cggagaagga agacctgtcg atctccgaca ggctctcgac ctgtatcgta aagttcagtc
     3841 atccggaacc agagatgtca gtcaggaaat tcgtgagact gaagctttac tgtagaaata
     3901 aaaacaaggg ccggggaatt tcattctgcc catattctct ttattgctgt gcgaaatcag
     3961 actattacgg ctcaaacggg cgtctctgga actggtcata accacatttc gggcatagcg
     4021 tcagcacttc cggtgtgtag atcgggagat ggaagtgaca tttctcgcag accagatttc
     4081 ccagcccgac cacttctccg ctgtgataaa ccccatgatg attgaggtcc tggaaaattt
     4141 cgcgccattc aagctgcgtt ttatcggtga tgtctgccag ctcctgccac aagctttctt
     4201 taatcacccg cataaagacg ctgtcagatt cttctttcag gctctcttca tagctcatgg
     4261 cgaactcttc caggtcacgt ctgacagctc gcgtcagctc atcgatctcg gttcgcgtta
     4321 actcccctgt ttttattacg cgctcgcgcg cctgttccac cagtgcgtcg atatcacgtt
     4381 cgccattgcg caggcgttcg ttcagtgacg cgaccagttc acggtaatat tgagcaacct
     4441 tgttcatcgt ttcatctccc gggtcgttaa ctgactataa taatagacgt tctttgttcc
     4501 ttgctttgct aatacgccca caaactctgt aaattaatgt acgaataatc cgggcatcat
     4561 ttcaacgtcg agtgcgcgaa aggctgtttt gacgcggacg tttgggctat gctatgcgga
     4621 tctgaaaaac cacctcaacg ctacatttgt agccgtattg aaaacaggac cactggctgc
     4681 catgcaagag caataccgcc cggaagagat agaatccaaa gtacagcttc attgggatga
     4741 gaagcgcaca tttgaagtaa ccgaagacga gagcaaagag aagtattact gcctgtctat
     4801 gcttccctat ccttctggtc gactacacat gggccacgta cgtaactaca ccatcggtga
     4861 cgtgatcgcc cgttaccagc gcatgctggg caaaaacgtt ctgcagccga tcggctggga
     4921 cgcgtttggt ctgcctgcgg aaggcgcggc ggtgaaaaac aacaccgctc cggcaccgtg
     4981 gacgtacgac aacatcgcgt atatgaaaaa ccagctcaaa atgctgggct ttggttatga
     5041 ctggagccgc gagctggcaa cctgtacgcc ggaatactac cgttgggaac agaaattctt
     5101 caccgagctg tataaaaaag gcctggtata taaaaagact tctgcggtca actggtgccc
     5161 gaacgaccag accgtactgg cgaacgaaca agttatcgac ggctgctgct ggcgctgcga
     5221 taccaaagtt gaacgtaaag agatcccgca gtggtttatc aaaatcactg cttacgctga
     5281 cgagctgctc aacgatctgg ataaactgga tcactggcca gacaccgtta aaaccatgca
     5341 gcgtaactgg atcggtcgtt ccgaaggcgt ggagatcacc ttcaacgtta acgactatga
     5401 caacacgctg accgtttaca ctacccgccc ggacaccttt atgggttgta cctacctggc
     5461 ggtagctgcg ggtcatccgc tggcgcagaa agcggcggaa aataatcctg aactggcggc
     5521 ctttattgac gaatgccgta acaccaaagt tgccgaagct gaaatggcga cgatggagaa
     5581 aaaaggcgtc gatactggct ttaaagctgt tcacccatta acgggcgaag aaattcccgt
     5641 ttgggcagca aacttcgtat tgatggagta cggcacgggc gcagttatgg cggttccggg
     5701 tcacgaccag cgcgactacg agtttgcctc taaatacggc ctgaacatca aaccggttat
     5761 cctggcggct gacggctctg agccagatct ttctcagcaa gccctgactg aaaaaggcgt
     5821 gctgttcaac tctggcgagt tcaacggtct tgaccatgaa gcggccttca acgccatcgc
     5881 cgataaactg actgcgatgg gcgttggcga gcgtaaagtg aactaccgcc tgcgcgactg
     5941 gggtgtttct cgccagcgtt actggggcgc gccgattccg atggtgacgc tggaagacgg
     6001 taccgtaatg ccaaccccgg acgaccagct gccggtgatc ctgccggaag atgtggtgat
     6061 ggacggcatt accagcccga ttaaagcaga tccggagtgg gcaaaaacta ccgttaacgg
     6121 tatgccagcg ctgcgtgaaa ccgacacttt cgacaccttt atggagtcct cctggtacta
     6181 tgcgcgctac acttgcccag agtacaaaga aggtatgctg gattccgaag cggctaacta
     6241 ctggctgccg gtggatatct acattggtgg tatcgaacac gccattatgc acctgctcta
     6301 cttccgcttc ttccacaaac tgatgcgtga tgcaggcatg gtgaactctg acgaaccagc
     6361 gaaacagttg ctgtgtcagg gtatggtgct ggcagatgcc ttctactatg ttggcgaaaa
     6421 cggcgaacgt aactgggttt ccccggttga tgctatcgtt gaacgtgacg agaaaggccg
     6481 tatcgtgaaa gcgaaagatg cggcaggcca tgaactggtt tataccggca tgagcaaaat
     6541 gtccaagtcg aagaacaacg gtatcgaccc gcaggtgatg gttgaacgtt acggcgcgga
     6601 caccgttcgt ctgtttatga tgtttgcttc tccggctgat atgactctcg aatggcagga
     6661 atccggtgtg gaaggggcta accgcttcct gaaacgtgtc tggaaactgg tgtacgagca
     6721 cacagcaaaa ggtgatgttg cggcactgaa cgttgatgcg ctgactgaag atcagaaagc
     6781 gctgcgtcgc gatgtgcata aaactatcgc taaagtgacc gatgatatcg gccgtcgtca
     6841 gaccttcaac accgcaattg cggcgattat ggagctgatg aacaaactgg cgaaagcacc
     6901 gaccgatggc gagcaggatc gcgctctgat gcaggaagca ctgcaggccg ttgtccgtat
     6961 gctcaacccg ttcaccccgc acatctgctt cacgctgtgg caggaactca aaggcgaagg
     7021 cgatatcgac aacgcgccgt ggccggttgc tgacgaaaaa gcgatggtgg aagactccac
     7081 gctggtcgtg gtgcaggtta acggtaaagt ccgtgccaaa atcaccgtcc cggtggacgc
     7141 aacggaagaa caggttcgcg aacgtgctgg ccaggaacat ctggtagcaa aatatcttga
     7201 tggcgttact gtacgtaaag tgatttacgt accaggtaaa ctcctcaatc tggtcgttgg
     7261 ctaagcgcgg gaggaagcgt gcgatatctg gcaacactgt tgttatctct ggcggtgtta
     7321 atcaccgccg ggtgtggctg gcatctgcgt gacaccacgc aggttccttc cactatgaag
     7381 gtcatgatcc tggactcagg cgatccgaac gggccattaa gccgtgcggt gcgtaaccag
     7441 ttacgtctga atggtgtcga gttgcttgat aaagaaacca cgcgtaagga cgttccatcc
     7501 ctgcgtttgg gtaaagtgag catcgcgaaa gataccgcat cggtattccg taacggtcaa
     7561 acagcagagt atcagatgat catgacggtg aatgcgaccg tgttgatccc cggccgtgat
     7621 atctacccga ttagcgccaa agtcttccgt tcattcttcg ataacccgca aatggcgtta
     7681 gcgaaagata acgaacaaga catgatcgta aaagagatgt acgaccgtgc tgccgaacag
     7741 ctgattcgta agctgccaag catccgtgct gcggatattc gttccgacga agaacagacg
     7801 tcgaccacaa cggatactcc ggcaacgcct gcacgcgtct ccaccatgct gggtaactga
     7861 tgattcggtt gtacccggaa caactccgcg cgcagctcaa tgaagggctg cgcgcggcgt
     7921 atcttttact tggtaacgat cctctgttat tgcaggaaag ccaggacgct gttcgccagg
     7981 tagctgcggc acaaggattc gaagaacacc acactttttc cattgatccc aacactgact
     8041 ggaatgcgat cttttcgtta tgccaggcta tgagtctgtt tgccagtcga caaacgctat
     8101 tgctgttgtt accagaaaac ggaccgaatg cggcgatcaa tgaacaactt ctcacactca
     8161 ccggacttct gcatgacgac ctgctattga tcgtccgcgg taataaatta agcaaagcgc
     8221 aagaaaatgc cgcctggttt actgctcttg cgaatcgcag cgtgcaggtg acctgtcaga
     8281 caccggagca ggctcagctt ccccgctggg ttgctgtgcg cgcaaaacag ctcaacttag
     8341 aactggatga cgcggcgaat caggtgctct gctactgtta tgaaggtaac ctgctggcgc
     8401 tggctcaggc actggagcgt ttatcgctgc tctggccaga cggcaaactg acattaccgc
     8461 gcgttgaaca ggcggtgaat gatgccgcgc atttcacccc ttttcattgg gttgatgctt
     8521 tgttgatggg aaaaagtaag cgcgcgttgc atattcttca gcaactgcgt ctggaaggca
     8581 gcgagccggt tattttgttg cgcacattac aacgtgaact gttgttactg gttaacctga
     8641 aacgccagtc tgcccatacg ccactgcgtg cgttgtttga taagcatcgg gtatggcaga
     8701 accgccgggg catgatgggc gaggcgttaa atcgcttaag tcagccgcag ttacgtcagg
     8761 ccgtgcaact cctgacacga acggaactca ccctcaaaca agattacggt cagtcagtgt
     8821 gggcagagct ggaagggtta tctcttctgt tgtgccataa acccctggcg gacgtattta
     8881 tcgacggttg atatgaaatc tttacaggct ctgtttggcg gcacctttga tccggtgcac
     8941 tatggtcatc taaaacccgt ggaaacgctg gcgaatttga ttggtctgac gcgggtcaca
     9001 atcatcccta ataatgttcc tccgcatcgt ccccagccgg aagcgaacag cgtgcagcgt
     9061 aaacacatgc ttgaactggc gattgccgac aagccattat ttactcttga tgaacgcgag
     9121 ctaaagcgca atgccccctc ttacactgcg caaacactga aagagtggcg gcaggaacaa
     9181 ggaccggacg tgccgctggc gtttattatt ggtcaggatt cattgctgac ctttccgacc
     9241 tggtacgaat acgaaacgat actcgacaat gcacatttga tcgtctgtcg gcgtccaggt
     9301 tacccacttg aaatggcgca accgcaatac cagcaatggc tggaagatca tttgacacat
     9361 aacccggaag atcttcacct tcagcctgcc ggtaaaattt atctggctga aacgccgtgg
     9421 tttaacatct cggcgaccat catccgcgaa cgtttgcaaa acggtgaatc gtgtgaggat
     9481 ttattgccgg aaccggtatt gacttacatt aaccaacaag gcttgtatcg ctgatacctg
     9541 tcgttacgga gggcatgatg cgactgtggt taattcgtca tggcgaaaca caagcgaatg
     9601 tcgatggtct gtacagcggt catgcgccca cacccctgac cgcacgcggt attgagcaag
     9661 cgcaaaatct gcatacactg ctagatgatg tttcctttga cttggtttta tgcagtgaac
     9721 tggaacgggc acagcatacc gcgcgactgg ttctcagtga ccgccagctc cccgttcata
     9781 tcatccctga actcaacgaa atgttttttg gcgactggga gatgcgacat catcgcgacc
     9841 tcatgcaaga agatgccgaa aactatagcg cgtggtgcaa tgactggcag catgctatcc
     9901 ccacgaacgg tgaaggattt caggcatttt cgcaacgtgt ggaacgcttt atcgcaaggc
     9961 ttagtgaata tcagcactat cagaatattt taatcgtcag ccatcagggc gtactgagtc
    10021 tgttaattgc tcgtttaatt ggcatgcctg ccgagtctat gtggcatttt cgcgttgacc
    10081 aggggtgttg gagcgccatt gatatcaacc aaaaattcgc gacgctacgc gtcctcaata
    10141 gccgtgccat cggggtcgag aacgcatgac ttttctgttt ttttacgggt aagccgcaac
    10201 gaccattgac agccccgggc aggctgatat tctccgcagc cagacttttt ccgccagaca
    10261 cgactttgta gaaattgttt tacaaaaatg gcgatgcaat ctgcggcgcg gggtgggatg
    10321 atagcccact ttcgaaagcc gattcggcga caattgtccc gaaatcgcct ctggttcagg
    10381 tatactgaca gaccattttt atctatttga ttcacccagg gggaaaactt gcagggtaaa
    10441 gcactccagg attttgttat cgacaaaatt gatgacctca aaggtcagga catcatcgcc
    10501 ttagacgttc aaggcaaatc cagcatcacc gactgcatga tcatctgtac gggtacgtcc
    10561 agccgtcatg ttatgtccat tgctgaccac gttgtgcagg agtctcgcgc agcgggcctg
    10621 ttaccgctcg gcgtagaagg tgaaaacagc gccgactgga ttgtcgtgga tttgggcgat
    10681 gtgattgtcc atgtcatgca ggaagagagc cgtcgcctgt atgaactgga aaaactctgg
    10741 agttaatgcg tgaagctgca acttgtcgcc gtgggaacga aaatgccgga ctgggtacaa
    10801 accggtttta ccgagtacct gcgtcgtttt ccgaaagata tgcccttcga gctgattgaa
    10861 attccggccg gcaaacgcgg caagaatgcg gacatcaagc gcatactcga caaagagggt
    10921 gagcagatgt tggcggccgc aggcaaaaac cgcattgtca ccctcgatat tccaggcaag
    10981 ccctgggata cgccgcagtt agccgctgag ctggaacgct ggaagctgga tggtcgcgac
    11041 gtaagtctac tgattggcgg gcctgaaggg ttgtcgcctg cctgtaaagc ggcggctgag
    11101 cagagctggt cgctgtcggc gcttaccctc ccccatccgc tggttcgcgt gctggtcgca
    11161 gagagtctgt accgggcgtg gagcatcacc accaaccatc cttatcaccg tgagtgataa
    11221 gggagctttg agtagaaaac gcagcggatg aaactacaga actcttttcg cgactatacg
    11281 gcagagtccg cgctgtttgt gcgccgggcg ctggtcgcct ttttggggat tttgctgctg
    11341 accggcgtgc ttatcgccaa cctgtataat ctgcaaattg ttcgctttac cgactaccag
    11401 acccgctcta atgaaaaccg cattaagctg gtgcctatcg cgcccagccg cggcattatc
    11461 tacgaccgta acggtatccc tctggccctc aaccgcacta tctaccagat agaaatgatg
    11521 ccggagaaag tcgataacgt gcagcaaacg ctggacgctt tgcgcagcgt ggtagatctg
    11581 accgatgacg atattgctgc attccgaaaa gagcgcgcac gttcacaccg tttcacctct
    11641 attccggtga aaactaacct aaccgaagta caagtagctc gctttgccgt caatcagtac
    11701 cgttttccgg gtgtcgaagt taaaggctat aaacgtcgtt actatcctta cggttcggcg
    11761 ttgacccacg tcatcggcta tgtgtcgaaa atcaacgata aagacgtcga acgcctgaat
    11821 aatgacggca aactggccaa ctatgcggca acgcatgata ttggtaagct gggcattgag
    11881 cgttactatg aagacgtgct gcacggtcag accggttatg aagaggttga agttaacaac
    11941 cgtgggcgtg ttatccgcca gttaaaagaa gtaccaccgc aagccggaca cgatatttac
    12001 ctgacgctgg atctcaaact ccagcaatat attgaaacgc tgctggcggg tagccgcgca
    12061 gctgtggttg ttaccgaacc gcgtacaggt ggggtgctgg cgctggtttc cacgcctagt
    12121 tatgacccaa acctgtttgt tgacggtatc tccagcaaag attattccgc cttgctgaac
    12181 gacccgaata caccgctggt gaaccgcgcc acacaggggg tttatcctcc cgcgtctaca
    12241 gttaaaccct atgtggcggt ttcggcatta agcgccgggg tgatcacgcg caatacgacg
    12301 ctgtttgacc caggctggtg gcaactacca gggtcggaaa aacgttatcg tgactggaaa
    12361 aaatggggcc acgggcgtct gaatgtcaca agatcgctgg aagaatctgc ggataccttc
    12421 ttctatcagg tggcctacga tatggggatc gatcgcctct ccgaatggat gggtaaattc
    12481 ggttatggtc attacaccgg tatcgacctg gcggaagaac gttccggcaa catgcctacc
    12541 cgcgaatgga aacagaaacg ctttaaaaaa ccgtggtatc agggtgacac cattccggtt
    12601 ggtatcggcc agggttactg gacagcgacc ccaatccaga tgagtaaggc actgatgatc
    12661 ctgattaatg acggtatcgt gaaggttccg catttgctga tgagcaccgc cgaagacggc
    12721 aaacaggtgc catgggtaca accgcatgaa ccgcccgtcg gcgatattca ttccggttac
    12781 tgggagctgg cgaaagacgg tatgtacggt gttgctaacc gccctaatgg tacggcgaat
    12841 aaatactttg ccagcgcacc gtacaaaatt gcggcgaaat ctggtaccgc tcaggtcttc
    12901 ggtctgaaag cgaacgaaac ctataatgcg cacaaaattg ccgagcgttt acgtgaccac
    12961 aaactgatga ccgcctttgc gccatacaac aatccgcaag tggctgtcgc catgattctg
    13021 gagaacggcg gcgcgggtcc ggcggttggt acgctgatgc gccagatcct cgaccacatt
    13081 atgctgggtg ataacaacac cgatctgcct gcggaaaatc cagcggttgc cgcagcggag
    13141 gaccattaat catgacggat aatccgaata aaaaaacatt ctgggataaa gtccatctcg
    13201 atcccacaat gctgctgatt ttactggcat tgctggttta cagcgccctg gttatctgga
    13261 gcgccagcgg tcaggatatt ggcatgatgg agcgtaaaat cggccaaatc gcgatgggtc
    13321 tggtcatcat ggtggtgatg gcgcaaattc ctccacgcgt ttatgaaggc tgggccccct
    13381 atctctatat catctgtatt attttgctgg tggcggtaga tgctttcgga gccatctcta
    13441 aaggcgctca acgctggctg gacctcggta ttgttcgttt tcagccgtcg gaaattgcca
    13501 aaatagccgt accactgatg gttgcgcgct ttatcaaccg cgacgtttgc ccgccatcgt
    13561 tgaagaatac tggcatcgcg ctggtgctga tatttatgcc cacgctgctg gtggctgcac
    13621 agcctgacct gggaacatca atcctcgttg cgctttccgg tctgtttgta ctgttcctct
    13681 ctggccttag ctggcgtctg attggcgtcg cagtagtgct ggtagcggcg ttcattccga
    13741 ttctgtggtt cttcctgatg catgattacc agcgccagcg cgtaatgatg ctcctggacc
    13801 cggaatcaga cccactcggc gcgggctatc acattattca gtctaaaatt gctattggct
    13861 ccggcggatt acgcggcaaa ggctggctgc acggcactca gtcacagctt gaatttctcc
    13921 ccgaacgcca tactgacttt atcttcgcgg tactggcgga agagctggga ttagtgggca
    13981 ttctgattct gctcgctctc tacattctgc tgatcatgcg cgggctgtgg atagccgcca
    14041 gagcgcaaac cacctttggt cgcgtcatgg ctggtggctt aatgctgata ttattcgttt
    14101 atgtcttcgt aaatattggt atggtaagcg gtattctgcc ggttgtaggg gttccgctcc
    14161 cactggtcag ttatggagga tcggcgctaa ttgtgctgat ggctgggttc gggattgtaa
    14221 tgtcaatcca cacccacagg aaaatgttgt cgaaaagcgt gtaagaggtg cgcaatgcgt
    14281 aagcagtggc tcgggatctg catcgcggca ggaatgctcg cggcatgtac aagcgatgat
    14341 ggtcagcaac agacggtaag tgtaccgcag cctgcggtat gtaacggccc catagttgaa
    14401 attagcgggg cggacccgcg tttcgaacca ctgaacgcga cggcaaatca ggattaccag
    14461 cgcgacggta aaagctacaa aatcgtgcag gatccgtctc gatttagcca ggcgggactg
    14521 gcggcaatct atgatgccga accaggcagt aacctgacgg cctctggcga agctttcgat
    14581 ccgacaaagc tgacggcggc ccatccaacg cttccgatcc ccagctacgc cagaatcact
    14641 aacctggcta acgggcgaat gatcgtggtg cgcattaatg atcgcggccc ttacggcaac
    14701 gaccgcgtta tttcgctttc tcgcgcggca gctgaccgtc ttaacacgtc aaacaacacc
    14761 aaagttcgta tcgatccgat tattgtcgcc caggatggtt cgctttctgg tcctggtatg
    14821 gcttgtacca cagtcgccaa acagacttac gccctgcctg cacctcccga tttaagcggt
    14881 ggcgcgggaa caagttcagt gtctggcccg cagggtgaca ttcttccggt cagtaattcg
    14941 acgctaaaaa gcgaagatcc gaccggcgcg ccggtaacca gtagcggttt cctcggcgct
    15001 ccaacgacct tagcgcctgg tgtactggaa ggcagcgaac cgacgcctgc gccacagccc
    15061 gttgttacag cttcgtcgac aacgcctgca acctcgcctg caatggtgac accgcaagcc
    15121 gcctcgcaaa gcgccagcgg caactttatg gtgcaagtcg gggccgtaag cgatcaggct
    15181 cgtgcgcaac agtaccaaca gcaactggga cagaagttcg gcgtccccgg tcgcgtaact
    15241 caaaatggcg cggtctggcg gatccagctt ggcccattcg ccagcaaagc cgaagccagt
    15301 accttgcagc aacgtttgca aaccgaagcc caattacagt catttattac taccgcgcag
    15361 tagcgtaaag caggcatctg atgtgtcaat ttctgtaaat gacgttaaca aagtcacgcg
    15421 gaaagtcaga tgcctgccgg tagtgcattt gctatagtag ggcacttttt taattccatc
    15481 acggatgtcg tagttcagac catgaatacc attttttccg ctcgtatcat gaagcgcctg
    15541 gcgctcacca cggctctttg cacagccttt atctctgctg cacatgccga tgacctgaat
    15601 atcaaaacta tgatcccggg tgtaccgcag atcgatgcgg agtcctacat cctgattgac
    15661 tataactccg gcaaagtgct cgccgaacag aacgcagatg tccgccgcga tcctgccagc
    15721 ctgaccaaaa tgatgaccag ttacgttatc ggccaggcaa tgaaagccgg taaatttaaa
    15781 gaaactgatt tagtcactat cggcaacgac gcatgggcca ccggtaaccc ggtgtttaaa
    15841 ggttcttcgc tgatgttcct caaaccgggc atgcaggttc cggtttctca gctgatccgc
    15901 ggtattaacc tgcaatcggg taacgatgct tgtgtcgcca tggccgattt tgccgctggt
    15961 agccaggacg cttttgttgg cttgatgaac agctacgtta acgcactggg cctgaaaaat
    16021 acccacttcc agacggtaca tggtctggat gctgatggtc agtacagctc cgcgcgagat
    16081 atggcgctga tcggccaggc attgatccgt gacgtaccga atgaatactc gatctataaa
    16141 gaaaaagaat ttacgtttaa cggtattcgc cagctgaacc gtaacggcct gttatgggat
    16201 aacagcctga atgtcgacgg catcaaaacc ggacacactg acaaagcagg ttacaatctt
    16261 gttgcttctg cgactgaagg ccagatgcgc ttgatttctg cggtgatggg tggacgtact
    16321 tttaaaggcc gtgaagccga aagtaaaaaa ctgctaacct ggggcttccg tttcttcgaa
    16381 accgttaacc cactgaaagt aggtaaagag ttcgcctctg aaccggtttg gtttagtgat
    16441 tctgatcgcg cttcgttagg ggttgataaa gacgtgtacc tgaccattcc gcgtggtcgc
    16501 atgaaagatc tgaaagccag ctatgtgctg aacagcagtg aattgcatgc gccgctgcaa
    16561 aagaatcagg tcgtcggaac tatcaacttc cagcttgatg gcaaaacgat cgagcaacgc
    16621 ccgctggttg tgttgcaaga aatcccggaa ggtaacttct tcggcaaaat cattgattac
    16681 attaaattaa tgttccatca ctggtttggt taaaaattga acacttgaaa gtgtaatttc
    16741 cgtccccata tactaagcat cagtaaaaaa actcccgcct tctggcggga gttgctattt
    16801 aattacgtta cgccggagct gacatgaaaa ccaaacttaa cgaactgctt gaattcccta
    16861 ctccttttac ttacaaagtt atggggcagg cgttacctga gctggttgat caggtggttg
    16921 aagtggtaca gcgccatgcg ccaggtgact acaccccaac ggtaaaacca agcagcaaag
    16981 gcaactacca ctcggtatct atcactatca acgccactca tatcgagcag gttgaaacac
    17041 tgtatgaaga actgggcaaa atcgatattg tccgcatggt tctgtaactc gcttctccgt
    17101 tacccggctc ctggtcgggt aactcccctc tcctcgctgt gatatacttt tcccccactt
    17161 ttactcattc tccacggaga tgccgttttg tatcaggata aaattcttgt ccgccagctc
    17221 ggtcttcagc cttacgagcc aatctcccgg gctatgcatg aattcaccga tacccgcgat
    17281 aatagtaccc ttgatgaaat ctggctggtc gagcactatc cggtattcac ccaaggtcag
    17341 gcaggaaaag cggagcacat tttaatgccg ggtgatattc cggtgatcca gagcgatcgc
    17401 ggtgggcagg tgacttatca cgggccggga caacaggtga tgtatgtgtt gcttaacctg
    17461 aaacgccgta aactcggtgt gcgtgaactg gtgaccttgc ttgagcaaac agtggtgaat
    17521 accctggctg aactgggtat agaagcgcat cctcgggctg acgcgcctgg tgtctatgtc
    17581 ggggaaaaga aaatttgctc actgggttta cgaattcgac gcggttgttc attccacggt
    17641 ctggcattaa acgtcaatat ggatctttca ccatttttac gtattaatcc ttgtgggtat
    17701 gccggaatgg aaatggctaa aatatcacaa tggaaacccg aagcgacgac taataatatt
    17761 gctccacgtt tactggaaaa tattttagcg ctactaaaca atccggactt cgaatatatt
    17821 accgcttaat tccatacatc aatggcccaa tttacactgg gtcattactc ataatacctg
    17881 ctaatgcttt acatctcctt cgcgtttgta tattttcttt atcacttgca gaaatgcggt
    17941 gccacgcctt gctccgtaac tcaccccatt gattaaccta ttgaaatagt tgattatggt
    18001 gagcaaaagc gttccgttcc aattagacat aagattattg taatttttta aatattattt
    18061 ttccatgaat aaaattggag taagtgcgtg gatagtaata atcaaattga accctgttta
    18121 agcagaaagt cgtcagaagg taaaccacaa atatttacaa ccttacgaaa tattgatctt
    18181 aaccttctga ctatttttga agctgtatat gtacataaag ggatcgttaa tgcagcgaaa
    18241 gtgcttaatc tgaccccctc ggcaatcagt cagtctattc agaaactgcg cgttatattc
    18301 cctgacccat tgtttattcg caaaggccag ggtgtcactc ctaccgcatt tgcgatgcat
    18361 ctacatgagt atatcagtca gggccttgag tccattcttg gcgcgctgga tatcgaagga
    18421 agctatgata agcaacgaac gataactatt gcgaccactc cctcggtcgg agccctggtc
    18481 cttcctgtca tctatcgggc gattaaaact cactatccgc agcttttact gcgcaacccg
    18541 cccatcagcg acgcggaaaa ccaactcagt cagtttcaaa ccgatctcat catcgataac
    18601 atgttttgca ccaatcgtac ggtgcaacat catgttctgt tcaccgacaa tatggtgtta
    18661 atttgccgca agggaaatcc actactctct ttagaagatg acagagagac tatcgacaac
    18721 gctgcgcatg tacttctgtt accggaaggg caaaatttca gcggtctgcg gcagagagtt
    18781 caagagatgt ttccggaccg gcaaatcaat ttcaccagct acaacatttt gacaatcgct
    18841 gcactggttg ccaacagtga catgttagcg attattccca gccgttttta taacctgttt
    18901 agtcgttgct ggccgctgga aaaactgcct tttccgtcct taaatgagga gcaaatagat
    18961 ttctctatcc actacaacaa attcatcctg cgtgatccca tcctgcacgg agtaatagat
    19021 gttatccgta atgcattttg aaaaagtatt gttacagcca gatcacagcg cagaacgata
    19081 aaatggcaca atccaacaac aattttacat tttcgcgacc gctttggctg ctttcaggtc
    19141 cgtttcaatg atatactgcc agtcgttaat tcaaaaatag ttgataatta caacaatcta
    19201 ttgaattgaa acgctttcct tcgtaattcg caactggaac acgcacgcta tgagtaaacc
    19261 cattgtgatg gaacgcggtg ttaaataccg cgatgccgat aagatggccc ttatcccggt
    19321 taaaaacgtg gcaacagagc gcgaagccct gctgcgcaag ccggaatgga tgaaaatcaa
    19381 gcttccagcg gactctacac gtatccaggg catcaaagcc gcaatgcgca aaaatggcct
    19441 gcattctgtc tgcgaggaag cctcctgccc taacctggcg gaatgcttca accacggcac
    19501 agcaacgttt atgatcctcg gcgctatttg tacccgccgt tgtccgttct gtgatgttgc
    19561 ccacggtcgc ccggtagctc ctgatgccaa tgaaccagtg aaactggcgc agaccattgc
    19621 cgatatggcg ctgcgttatg tggttatcac ctccgttgac cgtgatgacc tgcgcgatgg
    19681 cggtgcccaa cactttgcgg attgtattac tgccattcgg gaaaaaagcc cgcaaatcaa
    19741 aattgaaact ctggtgccgg atttccgcgg tcgaatggat cgtgctctgg atattctgac
    19801 tgcaacgccc ccagatgtgt tcaaccataa cctggaaaac gtaccgcgta tttaccggca
    19861 ggtacggcct ggcgcagatt acaactggtc gctgaagctg ctggaacgct ttaaagaagc
    19921 gcatccggaa atcccgacca agtctggtct gatggtgggt ctgggtgaaa ccaatgaaga
    19981 aattattgag gtaatgcgcg acctgcgccg tcatggtgtg acgatgttaa cgctggggca
    20041 atatttgcag ccaagccgcc atcacctgcc ggttcaacgt tacgttagcc cggatgagtt
    20101 cgacgaaatg aaagccgaag cgctggcgat gggctttacc catgctgcat gcggtccgtt
    20161 tgtccgctct tcttaccacg ccgatttgca ggcgaaagga atggaagtta agtaatcctg
    20221 taataattac ttacatctgt ctgataaaaa acccgctttt tgaagcgggt tttttgtatc
    20281 gaacaagata ttgagggagc gtcctgctcg ccacgtcact ctttatgaga gagcttttca
    20341 gcctgaagat caacgtctgc gccttttttc gccgcagcat cgtcatcatt catcgccttc
    20401 ttgaaccctt taatggccgc tccaaggtct ccgcccagcg tacgtaactt cttagtccca
    20461 aacagcagaa cgaccagcgc cgcaactacc agcagtttgg taatactaat ctcacccata
    20521 gataccttct tgacatataa ccggcaacag aacgcgcctt aaaaaccaga gaatattaac
    20581 ggtcattcga cgcgcgcaca caatagcaat ctgtaacaag gtgaatcaag cgccgagtaa
    20641 aaaaacatca taataattgc ggcggcgcaa agcgacggtt gtttaagatg ggcagttgcg
    20701 cgcgcacctg acgcacacgt tcgggcgtca cttccgccat aatgagtgca ggcatttctg
    20761 acgctgccgc aatggtgacg ccaaagggat ctataatccg gctttgcccg atatttttgt
    20821 tcccgcactc ccccgccgcc accatataac aggtggtatc cagcgcacga gcggcaagca
    20881 acgttgacca gtgatgctct ttgagcggcc cacgaaccca ggcggcagga agtaccagga
    20941 tttcagctcc ctgtaaagcc tgtgccagcg ccagctctgg aaagcgtaag tcataacagg
    21001 tcatcagacc gaccttcatc ccttccacct ccagtaacgg agctatttca ttaccagcat
    21061 caacatggcg tgattcctga atggcaaatg catcatagag atgcagtttg gcataacggg
    21121 cgacgatgtt tcctgcctga agtgccacca gcatattcca tgcgcgcccc ggcgttgaag
    21181 gaagatgaat cgtcagaatt gtcgtcatca tgttacgttt actttctcgc cgtaaacgtc
    21241 cgaggaattc gccttccagc agctgtgctg atttaaccga tagatctgca tcatgatcat
    21301 cgcgcgccag caatgcttcc ggcaggacaa acagcgatac gtcgttttcc gccgcctgcg
    21361 ccatcaacga ggcacaaatc tcagcgttct tttcccacac agatgtaaca gcaaactgtc
    21421 ctgcagcaac cagcattgcc acctcctgtc agagtcgtaa aattcgccaa cgacgattat
    21481 tcggcgttac acttatcact catacaaatc aaatagcagg attttgcagt gttacaactt
    21541 cttttagcag tttttattgg cggtggtacg ggaagcgtag cgagatggct gttaagtatg
    21601 cgatttaacc cgctgcatca ggcgattccg ttggggacgc tggcagcaaa cctgattggg
    21661 gcattcatca taggaatggg attcgcctgg ttcagcagga tgacgaacat tgatccagtg
    21721 tggaaagtat taatcaccac cggattttgt ggcggtctaa caaccttctc aacattttcg
    21781 gcagaagtgg tgtttttgtt acaagagggc cgctttggct gggcattact gaacgttttc
    21841 gtcaaccttc tggggtcttt tgccatgacc gcactggcat tctggctgtt ttcggcctca
    21901 accgtacact aaagaccaaa aaaaacccgc tgattaagcg ggttttgaat tcttgctgac
    21961 gtatcttaca gagcgattac gtttgcagca gaagggcctt tggcaccgtt agtgatttcg
    22021 aactctacgc gctgaccttc agcaagagtt ttaaaaccat tagtctggat tgcagagaag
    22081 tgtacgaaca cgtctttgct gccgtcttcc ggagtaatga aaccgaatcc tttggactca
    22141 ttaaaccact taacgttacc tttaatctta gacatcaaaa ttacctttac atgaaaaata
    22201 gacacaaatg ctgtgtcggt tatcagtaca ccaattgtgg tacgctttgt ccagtgcaac
    22261 tttactaaaa agtgataaaa gtcgcgttat ttttgatgtt gcttatttta gttattgatt
    22321 ttaaagaagt tactaaaact tcatttgtct caaaactgaa agcgcatcca ggcaaagtac
    22381 acattgccat tgttgtaggt acccggaatg taggtcatct gaaaagtcac tgggccataa
    22441 cccacggagg ccaatggcag tagaaccggg agagggatgt aattccagtt atcgcgtgcc
    22501 gttacgccag cggtgaatcc cagacctaaa tgaaaatttt catccgccag cggtcgccag
    22561 gtactttccc atccgtatcc ggcaatcggt tcccatttgt tccacgagtc cttaaatgcc
    22621 atggcataca ggccatgcca gtttcctttt tcatcccaac gcgacaggcc aaaaccgcca
    22681 ccccacggtc gctcgttata gcgatcggtt ttttctttgt cgtaagcgaa acgtgcatgc
    22741 caggtgatgg caggaatata taaatcataa tgttcaggct gttgccaggt ttgtgcaata
    22801 ttttctctaa acgttgtcat cctctcatct gcgttagcaa aaactttacc aacgctgatt
    22861 aactgaataa aaacaaagga aaagatagcg acatatttac tcacgttcat ttgtgaccat
    22921 aaaacattta tcaaaaatct act
//
LOCUS       DAZPCS010000048        22891 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZPCS010000048 DAZPCS010000000
VERSION     DAZPCS010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22891
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.048"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            144..1166
                     /locus_tag="ACHUQ7_001879"
     CDS             144..1166
                     /locus_tag="ACHUQ7_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HFR1135314.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
     gene            1166..2146
                     /locus_tag="ACHUQ7_001880"
     CDS             1166..2146
                     /locus_tag="ACHUQ7_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFR1135315.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENESV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLPIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWIGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            2143..2901
                     /locus_tag="ACHUQ7_001881"
     CDS             2143..2901
                     /locus_tag="ACHUQ7_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR1135316.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLSVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHTLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISR"
     gene            2911..3720
                     /locus_tag="ACHUQ7_001882"
     CDS             2911..3720
                     /locus_tag="ACHUQ7_001882"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR1135317.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTVYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRHAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYHWYQQQDARALAPASRDWALIWWDSVPQEAL"
     gene            3720..4574
                     /gene="modD"
                     /locus_tag="ACHUQ7_001883"
     CDS             3720..4574
                     /gene="modD"
                     /locus_tag="ACHUQ7_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HFR1135318.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
     gene            4600..6554
                     /locus_tag="ACHUQ7_001884"
                     /pseudo
     CDS             4600..6554
                     /locus_tag="ACHUQ7_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
     gene            complement(6604..6858)
                     /locus_tag="ACHUQ7_001885"
     CDS             complement(6604..6858)
                     /locus_tag="ACHUQ7_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFR1135319.1"
                     /translation="MGIIAWIIFGLIAGIIGKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIDGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            7059..7793
                     /locus_tag="ACHUQ7_001886"
     CDS             7059..7793
                     /locus_tag="ACHUQ7_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFR1135320.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            complement(7795..8406)
                     /gene="emtA"
                     /locus_tag="ACHUQ7_001887"
     CDS             complement(7795..8406)
                     /gene="emtA"
                     /locus_tag="ACHUQ7_001887"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFR1135321.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            8506..9420
                     /gene="ldcA"
                     /locus_tag="ACHUQ7_001888"
     CDS             8506..9420
                     /gene="ldcA"
                     /locus_tag="ACHUQ7_001888"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFR1135322.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINVHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGAQLTISGHPVLKM"
     gene            9515..11251
                     /locus_tag="ACHUQ7_001889"
     CDS             9515..11251
                     /locus_tag="ACHUQ7_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFR1135323.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            11862..13276
                     /locus_tag="ACHUQ7_001890"
                     /pseudo
     CDS             11862..13276
                     /locus_tag="ACHUQ7_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243131.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13444..14514)
                     /gene="dadX"
                     /locus_tag="ACHUQ7_001891"
     CDS             complement(13444..14514)
                     /gene="dadX"
                     /locus_tag="ACHUQ7_001891"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFR1135324.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQSDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGAPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(14524..15822)
                     /gene="dadA"
                     /locus_tag="ACHUQ7_001892"
     CDS             complement(14524..15822)
                     /gene="dadA"
                     /locus_tag="ACHUQ7_001892"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFR1135325.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGSTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            16152..17684
                     /locus_tag="ACHUQ7_001893"
     CDS             16152..17684
                     /locus_tag="ACHUQ7_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFR1135326.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNCLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGCATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            complement(17736..18455)
                     /gene="fadR"
                     /locus_tag="ACHUQ7_001894"
     CDS             complement(17736..18455)
                     /gene="fadR"
                     /locus_tag="ACHUQ7_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFR1135327.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            18676..20217
                     /gene="nhaB"
                     /locus_tag="ACHUQ7_001895"
     CDS             18676..20217
                     /gene="nhaB"
                     /locus_tag="ACHUQ7_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFR1135328.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYNVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            20363..20893
                     /gene="dsbB"
                     /locus_tag="ACHUQ7_001896"
     CDS             20363..20893
                     /gene="dsbB"
                     /locus_tag="ACHUQ7_001896"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFR1135329.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(20939..22207)
                     /gene="umuC"
                     /locus_tag="ACHUQ7_001897"
     CDS             complement(20939..22207)
                     /gene="umuC"
                     /locus_tag="ACHUQ7_001897"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFR1135330.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVMLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(22207..22626)
                     /locus_tag="ACHUQ7_001898"
     CDS             complement(22207..22626)
                     /locus_tag="ACHUQ7_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFR1135331.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
BASE COUNT         5485 a         6008 c         5722 g         5676 t
ORIGIN      
        1 catttttaat gatagagagg gtcaacaggc ttgcggaagt cgtcacatct tcatgttttc
       61 aatagctaaa ctttgatatt gaacatgatt tttatcgata tatatgaaag tatatatcga
      121 ttttactttt caggatgttc accatgccca taaccagacg gacgtttgcg caggcacttg
      181 catctaccct gctcctgcag tctctaccgt ccttttcaca aacagttaat cgttttgctt
      241 cgcaatctct tcccgaagcc caaaacatta cacgcatcgt cagcgcaggc gcgcccgccg
      301 atttgctctt gctggctgtc gcgccagaaa aaatggtcgg tttttcctct tttgattttg
      361 cccgtcaggc attaattcca ttgccagagc acattcgcca gttacccagg ctgggacgac
      421 tcgccgggcg cgccagcaca ctatcgctgg aagggcttat ggcgttacat cccgatttgg
      481 tcgttgattg cggcaatacg gatgaaacct tgatctccca ggcacggcag gttagcgaac
      541 agacacaaat cccctggtta ttgcttaacg gaaaactggc gcaatcagca gaacagttaa
      601 caacgcttgg caaaacgtta ggcgaagagc accgcgccgc agaacaagcc aatctcgcca
      661 gccacttcgt tggtgaagct caggcattcg ccacctcacc cgccgctaac ctccgctttt
      721 atgctgcgcg cggtccccga gggctggaaa cgggcttaca gggttcgttg cataccgagg
      781 cggcggaatt attaggtttg cacaacgtcg cgcaaatagc cgatcgccac ggcctgacac
      841 aggtttccat ggaaaatctc ctgcgctggc agccggatat tattctggtt caggaggccg
      901 ttactgcaga ttttattcgt cgtgatcctc tctggcaggg cgtgaaagcc gttgcggaac
      961 aacgcatctt atttttaagt ggcttgccct ttggctggct cgatgccccg ccgggaatca
     1021 accgtcttct gggattacgc agacttcacg cctggctgga tcccgccatc aatcgccagt
     1081 ttaaaagtga catgcagcat tacgcccaac tgttctggca ttgttcactc agtgatgccg
     1141 actatcaaaa attggtggcg agctaatgag aatcgtaaac ggatgtattt tgctgctggc
     1201 agctatcagt atcacttttg ccgccgtaag tggggcttat catctcgata tgcagcaact
     1261 gctggcgcta attcttcgtc aagaaaatga gtctgtgcag gaacaaattg tcttctggca
     1321 aatccgcgtg ccgcgtattc ttgctgcgct gttactgggc gcagcgctgg caggagccgg
     1381 aaccacctat cagggaatgt tgcgtaatcc cttagtttca ccggatattt taggcgtctc
     1441 cgccggtgcc ggacttggtg cctgcgcggc aatcctgtgg ggattaccca ttgtttacat
     1501 ccagctgtac gcattttgcg gcgggctgat ggtggtcgcg ggcgtctggt tgattacgcg
     1561 tcgggtgacc cgccacgatc cgatcttaac cctggtgctg gtaggcattg cgttaggaac
     1621 gttgtgcgga gcggggatat cgctaattaa aacgctggcc gatccttaca cccagttacc
     1681 gtcaattacc ttctggttgc tggggggact gtccaccgtg accttacgcg atctctgcta
     1741 tgctgcaccg atcattctca taggctcctt gcccctgttt tttctccgct ggcgcatgaa
     1801 tttactcacg ctttccgacg acgaagcccg ttcgctggga ctgaacgtga cgcgtctgcg
     1861 ctttggattg attgtctgcg ccacactaat caccgccagc acggtagcaa tagcgggcat
     1921 aattggttgg atcggtctgg tcgttccgca cattgcccgc ttgttgaccg gtcataacca
     1981 tcagcagctg ttgcctatgg caatgtgtac tggcgctatt ctgctcctgc ttaccgatac
     2041 gctggcgcgc agcattggaa caacagaaat cccacttggc attctgaccg cttttgttgg
     2101 cgcaccgttt ttcctgcttt tgttgttacg cggaggtcgc cagtgacttt gcttgccgtt
     2161 cgtcatgcgt ctctgggtta ctcccgccat ccagtgctac gcgacgtgtc cttcaccctg
     2221 tcgcaaggaa caatttgttg tctgcttggc gcgaatggtt gcggcaaaac cacactcatg
     2281 cgctccattc ttggcgttat accgttatta aaaggcgaga tcctactgga tagcctctct
     2341 gtgcagacac tcagccatcg ccagcgcgca caggctattg cctgggtgcc gcaggcgcac
     2401 gacggtattt ttgctttcag tgtgctggat atggtgttga tgggcctggc ccccaccata
     2461 ggcgcttttt ccgttcccgg taagcaagaa cgcctgaaag ccacggagca actggaaaag
     2521 ctgggcattt tgcatctggc gacacgacgc tggaatacgc ttagcggcgg tgaacgtcag
     2581 ctggctttaa tcgcccgtgc actcgtccaa caaccgcgct tgttgttact ggatgagccc
     2641 gcctccagcc tcgatttcgg tcatcagatc caacttctgg acacgctggc gcagctcaaa
     2701 aataacggca tgacaatgtt aatgtcgact caccatacgc tccatgccaa cgccattgcc
     2761 gacagcatta tccaagtaga gccagatgga cgagtaacac aaggcttacc gaccgaacag
     2821 ttaacaacga acaaactggc ggccctgtat cgggtttctg ccgaccaaat tcatcatcac
     2881 ctctctgcaa ttagccgcta aggaacaatc atgctcattg atgatattga ttttgcagac
     2941 ctttatctgc agcaactcaa actggctcac cgcacagaaa aaacgcccga tcactgggat
     3001 caacgggcgg agaaaatggc ggaaaattgc gcctcgccaa cggacagcta cctgcaacaa
     3061 ctgatcgcta aaattgacct gcaaggcgct cagaccttat tcgatatggg ctgcggacca
     3121 ggcaccgtat cgctggcgct ggctgacaaa ctcactacgg tttatggcgt tgattacagt
     3181 caggggatgc tgaacgttgc cgcccgccgc gccgccgcac tgaaggctga caatgtccac
     3241 tggatccagc gcgcctggga agaggactgg agtgacctgc cacgctgcga tattgccgtg
     3301 gcttcccgct ccactctggt ggctgatatg cgccatgcaa tgagcaaact gaataatcag
     3361 gcacggcttc gcgtctatac aactcatctg gtcagcacct cgttcgtctc tccagccatt
     3421 cagcgggcgg ctggtcggga agtgatcgaa ttacccaact atatctttgc gctcaacgtc
     3481 ctgtatcaga tgggaattta tgcccacgtt gattttatcc gtggacaaaa ttgccaacag
     3541 gataacagca cctgggaacg ttttgagcag aacgtcagct ggagtcttgg cgcgcttaac
     3601 gacgatgagc gtgagcggtt atatcactgg tatcaacaac aagatgctcg tgccctcgcc
     3661 cctgccagcc gcgactgggc gctcatctgg tgggatagcg taccgcagga ggcactgtga
     3721 tgattttcct ttctcaggca caaatcgatg cgttactgct ggaagatatc cagggaggtg
     3781 acctgaccac gcgagcatta aatattggac accagcctgg ctatatagcg ttttttcacc
     3841 gtcagggcgg ctgcgtcagc ggcgtttctg tcgcgtgtaa gatgttaact acgctgggat
     3901 taactattga tgacgcggtc agcgacggtt cacaagcgaa cgcgggtcaa cggctaatcc
     3961 gtgcgcaggg taatgccgca gcacttcatc agggatggaa ggcagtccag aatgtgctgg
     4021 agtggagttg cggtgtttct gattatctcg ctcaaatgct ggcattgctt cgtgaacgtt
     4081 atcctgacgg caatatcgcc tgcacccgaa aagcaattcc gggcacccgc ttactggctt
     4141 cgcaggcaat tctggctgcc ggaggactaa ttcatcgtgc cggatgtgcg gaaaccatat
     4201 tattgtttgc caaccaccgc cattttcttc atgacaatca gggctggtca ggcgcaatcg
     4261 ctcagttacg tcgccacgca cccgagaaga aaattgttgt tgaagccgac acgccgaaag
     4321 aggctatcgc cgcgttacgc gcacaaccag acgtacttca gctcgacaaa tttagtccgc
     4381 aacaggcaac tgaaattgcg caaatagcgc cgtcgctggc tcctcactgc acgctggctc
     4441 tgaccggcgg aattaatctg acgacactca aaaattacct cgactgcggc attcgccttt
     4501 ttattacctc cgcgccttat tacgcggcac cagctgacat taaagtcagt ctgcaaccca
     4561 cagcctctat ttaattcttt gttaaacgga atttattcta tgagactcaa aaaacgttat
     4621 ctctgcacag cattatcact tgcctttacc cagcaggccg tagcggctca ggagagcgac
     4681 acgctgaccg tatggtccag tccggtatca tcgacgacga ccaccgttct cgatcaaccc
     4741 accatgaagg ccttggataa acagaatgtc gctcaggcat taagtgtcgt ccccggcgtg
     4801 gtgctgcaaa agtcaggcag ccgcaacgaa gaacaggtaa aagttcgtgg ctttgatagt
     4861 cggcaggtgc cggtctattt cgacggtgtg cccatttatg tcccctatga cggcaacctc
     4921 gatctggcgc ggattctgac caacaatctg ggggcagtcg aagtttccaa agggtattcg
     4981 tcgctgcttc atgggcctaa tcagatgggc ggagccatta atatcaccac cctgaagaag
     5041 caagtctggg atatcgccag ggatggagcc gtagtcagga caatgcctac gatatgcatg
     5101 cttcatttgc cgccagcagc gatcttgggt atttgcaagt cagcggtagc cagctaaagc
     5161 aggattttct cggcctgccg catggtgtaa ataatgatat tgcaggcaaa cacggcaaga
     5221 tgattaattc atcggctgat gataaacgcg gcattgtgaa gctcggtttt acaccgcgcg
     5281 aaaacgatga atacacgctg acttacatta agcaggatgg tgaaaaagat aacccgccat
     5341 acagcggaaa ttgtggtcaa aaatcacgct actggcagtg gccagagtat gacaaagaaa
     5401 gtttttatta tcagggagcg acccaactaa acgaccgttt taccctgaaa agtcggctgt
     5461 atcgcgacac ctttgaaaat acgctgatga tgtacaactc gctggctgat ttgaaaaata
     5521 aaaaaggcag ctacagccat tattccgatt acagcgacgg tgccggatta caactggcag
     5581 ccgatgtgcg tgaaaacgat ctgctgtcgt ttgccgttaa ctggaaagat gacgtacacc
     5641 gggaaaaagg tgcgccgcac gccgcttacg atcgctatga agatcgtacc tggtcgctcg
     5701 ccagtgaata tcaatgggct gctgctgata atgtcgatgt cgtggcggga atcagctatg
     5761 actggcgcga tagcgtagaa gcgaaaaaac acgagaaaga tggcagcatc acccattatg
     5821 acgacaacaa tcagtcagct tttaactggc aggtgatggg gaaataccac tttgccaatg
     5881 aagacactct ggcgctttcg tactatgacc gcacacgctt tccgacgctg aaagaacgct
     5941 ataccacatc caaacctgcg tataaccaga tagcgattgt taacccgcag ctcaagccgg
     6001 aacgcgctcg cggggtggat ttaacctgga atggtgcctt cacgcgcgac tggggatttg
     6061 aggtcagcgt ttactataac cgggtgagtg atgccatcct ctcgcacaat atcaatgccg
     6121 ataccattca aaatcagaac agcggcacgg tggattacag cggtctggat gccggtatta
     6181 aggggaaaat cagcaatata ctggatgtag gattgagcta cgctctgatc cacgctgacg
     6241 ccaaacgcaa agacatcggc aagataaccg atcttccaac gcagacaatg accgcatgga
     6301 tgacactcaa accatgggag ccgttaagcg taacgctgtc ggaagaggcg cgttcctcca
     6361 gctacagcaa cagtgacggt tcacaaaaag ccgccggttt tgcggtgacc cacattcgag
     6421 ccgattacac cttaggtcat ggcttcagcg tcaatgcgtc ggtcaataac ctgtttgata
     6481 cccaatatgc ctacagtgaa gggtttattg aagaaggtcg caatttctgg gcaggtgtcg
     6541 agtatacgtt ctgatgtgaa agcggatagc ggcatctgta tcgctatccg ccttatgaaa
     6601 atcttattct cttcgcagga ggcggaatac gcccagcacc aggatagcgc ccaccaccgc
     6661 caccaggaag ctgtgcaaat taaaaccgct gatggagccg tcaatgccaa acatggtcgc
     6721 cagccagccg ccgaccaccg caccgactat cccgagaata caggtcagga aaaatccacc
     6781 accatcacgc cccggcatga ttagcttgcc gataatgccg gctatcaggc caaaaataat
     6841 ccaggcaata attcccatgt tcaggccctc gtttcgatga atcggtcgca agaaatgctt
     6901 acgtcaacgc tctaagtata ggcaagggcg ggatcgatgc tgggcgtttg tcggctgatt
     6961 taagaacaac atgtaaaaaa tattcacgca tttgtattaa gttttgttaa ctatgaccga
     7021 taaaccaaag acagtttgtc agtcaggagt ttttccgcgt gagtcattac catgagcagt
     7081 tcctgaaaca aaatccgtta gccgtcctgg gcgtgttacg cgatttgcac aaagccgcaa
     7141 ttcctttgcg tctcagttgg aatggcgggc agctgatcag caaaatactg gcaataaccc
     7201 cggataaact ggtgctggat ttcggcagtc aagccgaaga caacatcgcc gtgctaaagg
     7261 cacagcacat taccattacc gccgaaactc agggtgcgaa agtcgagttt actgttgaac
     7321 aactacagca gagtgaatac ttgcagcttc cggcatttat taccgtaccg cctcccacct
     7381 tatggtttgt acaacgacgc cgatatttcc gcatctccgc cccactccat ccgccttatt
     7441 tttgccagac caaactggcg gataacagta cgttacgttt ccgcttgtat gatttgtcgt
     7501 taggcggcat gggcgcatta ctggaaacag caaagcctgc cgaattacaa gaaggtatgc
     7561 gcttcgctca aattgaagtc aacatggggc aatggggtgt ttttcacttt gacgcccagt
     7621 taatctccac cagcgagcgc aaagtgattg atggcaagaa tgaaaccatc accactcccc
     7681 gtctgagctt ccgttttctt aacgtcagcc cgacggtgga gcggcaatta cagcggatta
     7741 ttttctctct cgagcgagaa gcccgggaaa aagcggacaa agtgcgcgac tgatttacat
     7801 cgcgtccagt gcctgctcaa gtttgtagat atagcgcgga gcctgcggcg caggatgatt
     7861 tcgcgctacg tgatcgagga actcgtcagc atccaaatcg ttgattttgc tgatcgcctt
     7921 tttccgatct gacgagaaag tccgtagcag cgcacctgcc ccgttagcgt atgacaccac
     7981 cagcgcatat tgcagtacct tcggatcttc aatgcctgcc agcgggccgg tttccagaat
     8041 attcaggtaa gccgccccca ttgaaatatt acgctctgga tttttcagct cgctggtcgt
     8101 cggctcacca ctccagccca tacggcgata aacatcacgt ccggaggttg aagcttttaa
     8161 ctgcatcaaa ccaatggcat tcgatttact caccgcgttc ggattaccac ccgattcgat
     8221 agcgataatc gccgtgatca attgtggatc gacgccccag gctgcaccgg ctttctggct
     8281 tattggcatc cactgcatcg cacgttgcac cggaactttc gcgttccacg gcgggttcgt
     8341 atagtcatgc tttgatgaac aacccgctaa taacacaatc aaaaaggcaa accatctcaa
     8401 tttcacttct tctatcctta tagcctggaa tcgccgttga ggtgacaacg gcacacctaa
     8461 gcggcatgat atacatttcg tttctgttgt cagcaaggaa ttgccatgtc tctgtttcac
     8521 ttaattgccc catcgggtta cagcattaaa cagcacgccg cgctgcgtgg tatccagcgc
     8581 cttaccgacg cggggcatca agtcaataac gtcgaggtta ttgcccgtcg ttgtgagcgt
     8641 tttgcgggta cggaaacgga gcgtctggaa gatctcaatt cccttgccag actgactacc
     8701 cccaacacca tcgtgctggc tgtacgcggc ggttacggtg ccagtcgttt actggcagat
     8761 attgactggc aggcactggt ggctcgccaa caacatgacc cgttactcat ttgcggacat
     8821 agcgatttta ccgccattca gtgcggtctt ctggcgcaag gcaatgtcat cacctttagc
     8881 ggccccatgc tggtagcaaa ctttggtgcg gatgaactga acgcctttac cgagcaccat
     8941 ttctggctgg cgttacgcaa tgaaaccttc accattgaat ggcaagggga aggcccgaca
     9001 tgtcagaccg aaggcacgtt gtggggaggc aatcttgcga tgctgatttc actgattggt
     9061 acaccgtgga tgccaaaaat tgagaacggt attctggtgc tggaagatat taacgtgcac
     9121 cctttccgcg tcgaacgtat gctgttgcag ctctatcatg cgggaatttt gccacgtcag
     9181 aaggcgatta ttctcggtag ctttagcggc agcacgccca atgattatga cgcaggttac
     9241 aacctggagt cagtgtacgc gtttttgcgt tcccgcctgt cgattccact cattaccggc
     9301 ctcgattttg gtcatgaaca acgcacggtt acgctgccgt tgggcgcaca tgccattctg
     9361 aataacaccc gggaaggcgc tcagttgacg attagcggtc atcctgttct taaaatgtaa
     9421 aaaaatcacg cttcagggct aagtaaaacg ctgtctctgc cgctaatatg gtagggttta
     9481 tataagagac agcgtaatca ggagtaaccg accattggat gccacaacaa taattagcct
     9541 tttcatctta ggatccatcc ttgtcaccag cagtatatta cttagttcat tttcttcccg
     9601 tcttggcatt cctattctgg ttatttttct ggcgatcggc atgctggcgg gagtcgatgg
     9661 cgtcggcggt atcccgtttg ataattaccc cttcgcctac atggtaagta acctggcgct
     9721 ggcgattatt ttgctcgacg gcgggatgcg cactcaggcc agttcctttc gtgtggcgtt
     9781 aggaccggcg ctgtcgctgg cgacgctggg cgtacttatc acctctggtt taaccggcat
     9841 gatggcggcg tggctgttta atcttgattt gattgaaggc ttattaatcg gcgctatcgt
     9901 cggctccacc gatgctgcag cggtcttttc tctgctgggt ggtaaggggc ttaacgaacg
     9961 tgttggctcg acgctggaaa ttgaatccgg cagtaatgat ccaatggcgg tctttctgac
    10021 gattaccctg attgcaatga tccagcagca tgaaagcagt gtcagttgga tgttcgtggt
    10081 cgatattctg cagcaatttg gcctcgggat tgtcatcggt cttggcggtg gttatttact
    10141 gctgcaaatg attaatcgca tcgccctgcc tgccggatta tatccattgc tggcattaag
    10201 cggcggtatt ttaatttttg ctttaaccac ggcgctggaa ggcagcggta ttctggctgt
    10261 ttatctgtgc ggttttctgc tgggtaatcg gccgattcgc aaccgctacg gcatcctgca
    10321 aaatttcgac ggcctcgcct ggctggcgca aatcgccatg ttcctggtgc tggggctatt
    10381 ggttaaccca agcgatctgc tgcctattgc cattccggcg ctcattttgt cggcatggat
    10441 gatattcttc gcccgtcccc tttcggtatt tgctggattg ctccctttcc gcggtttcaa
    10501 tctgcgtgag cgcgtgttta tcagctgggt gggattacgc ggcgcggtgc caatcatcct
    10561 agccgtgttc ccgatgatgg cggggctgga gaatgcgcga ctgttcttta atgtcgcctt
    10621 ctttgtggtt ctggtttcac tgctcttgca gggaacatca ctctcgtggg cggcgaaaaa
    10681 agccaaagtg gtggttccgc ccgtcggacg tccggtgtca cgcgttggcc tggatattca
    10741 tccggaaaat ccgtgggagc agtttgttta tcaattgagt gctgataaat ggtgtgtagg
    10801 tgcggcactg cgcgatttgc atatgccaaa agagacgcgt attgcagccc tgtttcgtga
    10861 taaccagttg cttcatccta ccggcagcac ccgactgcgc gaaggcgatg tattgtgtgt
    10921 aattggtcgg gaacgcgatc tcccggcgct cggtaaactg ttcagccagt cgccaccggt
    10981 cgcgctggat caacgcttct ttggtgactt cattctcgaa gccagcgcca aatatgctga
    11041 tgttgcgctg atatatggcc tggaagacgg gcgagaatat cgtgataaac agcaaacgct
    11101 gggtgaaatt gtccagcagt tgctaggcgc ggcaccggtt gtcggtgacc aggtagagtt
    11161 tgccgggatg atctggacgg tggccgagaa agaagataat gaagtgttga agattggcgt
    11221 tcgggtggct gaagaggaag ctgaatctta aatttaatgc agcatctatc gccggatgcg
    11281 atgctttact gaacgcagcc tgagcctgta ataaacttgt catttcctga ttttgatatg
    11341 ctcatcccaa catcagaaga aggttaaatt atggatgaca aaccattgca ggctctggct
    11401 aatgaactca aaacaccggc tcaagaaagt cagcgtaagg gctttttccg actgtaacag
    11461 gtaataaaaa ccagccccgc attaccggtc aaccatcagg ggcccctgcc tgacatcgcc
    11521 tgaacatatt cccccacgca acctgccgca ctgacatagc ccacacaata ggcgtttcat
    11581 ccgcctgtag caccggatgc atgagcgatt tcttatagtt attcctttgc ttaataacga
    11641 ttatttccgg gtcgttcact ctcgtatttt ctttaatacg tcagagctac ctaggccgta
    11701 atacagcatg taccagaccc gacacggcat ttctgaccag gaaaaaagtc aacgatgagt
    11761 agtgatatca gcgcaaatga cgtcctgtgg ctcctttgcg gtgaccacat tcacgtctcc
    11821 ggcgctgcca gaggatattt atttcaagga aacgtagcat tatggctagc tttaaagaca
    11881 tctgcccatt cgcccccgcc attcccccat cttccgttaa cccaggcgta cgacgcccgc
    11941 cagggcggct cttctcaaga atcaaatttt gcagaacatg aagtatctca gcccgcaaaa
    12001 attcagctgg ggtgatgccc cctggcagat cattgacctc agtatcgcgg gcaaagtgaa
    12061 catacaggtg gacaacaata ccatcatcac gttggggact cgtctgaatc agcaacataa
    12121 cgagttcatg atggtcgcca aatggtgcga atgggctata cagcaggatg gtttgcaaga
    12181 aaacctgcaa aaaaacctgt acgaaatcct ggaagaaaac cagcaaaaca aacagtcaga
    12241 aattccccaa gaagacctga aagaaagtct ggaagaaatc aaggaaaata tcctggaaga
    12301 aaacctgcca gcaagccgga ttgaaaaccg ggcagaagca ttgcgcagaa tgaaggaatg
    12361 tctcattaca agacggagta tgctcaatct tagcaacctg ggattgactt cactccctga
    12421 gaatttgcct ccacatctga ttgaatttta ctgcagtaaa aacgtgttga ccgcgttacc
    12481 gaaggtgatg ccaaagtggc tgctggtgct tgactgtaca gataacgtct tgatcttgtt
    12541 accgaaggtg cagccctcaa aactgatggt actgaagtgc tatgaaaaca gtattatctg
    12601 gctgcctgag ctgtcgacta acctgagagt gattaactgt tctgaaaact tcttgcaatt
    12661 tttaccgccg tcgatgcccc agtacctgta taaactatcc tgtgccggca acaatattaa
    12721 ctcaatacct gatgagatgc tggagaactt gactcgcctg aaagtatttg actgttctag
    12781 taacgatttg atctcttcac cacggctgcc gcccaaacta ataatttact actgtggaga
    12841 aaacaagttt aaaacggtac aggtgccgca gccccagagc ctgaaggtgt ttgactgtaa
    12901 tggtaacccg tgggacaaag acaatttacc gacgctgctc aaagccgtcg agggcctgaa
    12961 aaaacagcag ggcctgaagg accttttgga ctttttgcac aaggaaggct aggttgacct
    13021 ggaaggactc gaggagctgg aggaccttat ggatctggag ttcctggatg acccggaact
    13081 cctggagcgc gtgaaggtac aggaggacct ggagctcctg gatcaacagt tgggcctgtt
    13141 gagtctggaa agacaacagg actcgcagcc tgttaatcaa caatctgaac atgaacccga
    13201 atctgcatca agggtgaagc gtgatttatc tgaagtcgac tccgagtcaa caatgaagcg
    13261 taagcgtttt atgtagaacg cctgtgcggc gagttcaggt tccggggaaa tagtgtccat
    13321 tcttttaatg gacactatca tgggcaatgg ctctgtcaca ttaacaacta cagttgctga
    13381 ccagccggat ggatgatttt ttcgcaacca gaagacgctg cctccgatcc ggcttacaac
    13441 aagttacact gtcacaaccg ggacgcgtaa cgccagtgcg cacatcaact catagcccac
    13501 cgttccggca gcggcggcga catcatcaat tttgatctcc ttgccccaca gctcaaccgg
    13561 cgtaccaatc cccgcctgcg ggcaaggcgt taaatcaacc gccagcatat ccatcgagac
    13621 ggtccccacc gtcatggtgc gcacgccgtc cactaaaaca ggagcaccgg taggcgcatg
    13681 gcgcggataa ccgtcggcgt atcctgcggc gacaatgccg attcgctgtt catcgcgcgc
    13741 agtatagcga ccgccgtagc ccacacgctc gcccgctttt agcgtctgga caccaataat
    13801 ctcactgctt agcgtcatca ccggacgtaa tccggtattg gcgatatcac gccactgacc
    13861 ggacggcgaa gcgccataca aaataatgcc aggccgaacc cagtcaaaat gcgcttccgg
    13921 gtgccacaga gtcgccgccg aattggacaa cgaacgccga cactccagcc cctccgccgc
    13981 ctgctcaata cgcgccatcg cgccggaaat tccatcagga tgttccgctt cagcaaaatg
    14041 cgacatcagg gtcatttcgc caacattcgc cattgcccgc aactgctgcc agacggtaag
    14101 cacgcgatcg gactggaagc ccaaccgatt catcccactg ttcactttaa gataaatatc
    14161 caacggtgct tttagccgcg cattttgcag tgctttgagc tgccagttgc tgtgtacgca
    14221 ggtggtcagg cggtgctggt cataaatctc cagatcctga gcatggaaaa atccttccag
    14281 catcaggatc ggccccttcc agccgcgctc acgtaacgtt attgcctctt ccaggttaag
    14341 taatgcaaag ccatcggtgg ccccgagcgc gctccagata cgctcaatac catgcccgta
    14401 agcgttcgct tttaccaccg accagacgcg cgcgtgcggc gcggcctggc ggacaatgga
    14461 cagattctgt tttaatgcct gcagatcgag gctggcctgt atcggacggg tcatctcgtt
    14521 tccttagctg tgtgcgccat gtaaatggcc cggacgtgat ggcgtaaatc cacggctgta
    14581 gcgcgctacg cttagatcct catatgggat cgctggcgtg cgaccagaga gcagatcgct
    14641 taacaactga ccggaaccgc aagccatcgt ccagccgagc gtgccgtgac cggtattcag
    14701 ccacagattt ttaaagcgtg tactcccgac aaccggcgta ccgtctggcg tcatcgggcg
    14761 caaacccgtc cagaaagtcg cctgctcgac atgaccgccg cgtggataga gatcgcgaac
    14821 caccatttcc agcgtttcac gacgcggttg caacagctcg gtgttaaaac caacaatttc
    14881 cgccattccg ccaacacgaa tgcggttatc gaaacgggta atggcgattt tgtaggtttc
    14941 atcaagaatg gtggatactg gcgcaccatc ttcctgcgca attggaatag tcagcgagta
    15001 gcctttcagc ggatacaccg gaatatcaac aatgcctttg agcatcgccg tcgagtaaga
    15061 accaaacgcc atcacatacg catcggcctt aatcacttca tcgccaaact tcacgccgta
    15121 gatttgctcg ccgtcgcaaa gcagttggtc aacgggcgta ttaaagcgga atttaacccc
    15181 cgcctgctct gccatccgcg ccagattctg ggtaaacagc tggcagtctc cggtttcatc
    15241 attgggtaac tgcaggccgc ccgtcagttt gtgcgctact tctgccagcg cgggatccac
    15301 ttccgccagg cggctggatt ccagcagctg atacggtacg ccggcatctt ccagcacggc
    15361 gatatcgcgg gtcgcatttt catactgttg ttcggtacgg aacagttgca gcgtcccacc
    15421 ctgacgccct tcatactgaa tattggtttc ggcgcgcaat gctttcaggc aatcacggct
    15481 gtattccgcc agacgcacca tccgcccttt gttttccatg tagtggctgg tgtcgcagtt
    15541 acgtaacatt tgccacatcc atttcaactg aaactgcgta ccgtcgagac gaaccgccag
    15601 cggcgcatgg cgctggaaca tccatttaat cgcttttaaa ggcacacctg gtgctgccca
    15661 cggtgcagca tatccggggg agatttgccc ggcattcgcg gcactggttt ccagggcggc
    15721 ccccggctcc cgatcaatga cggtgacctc atgtcctgcc tgatttaagt accaggcgct
    15781 ggcaacgcct accacaccac ttcccagtat gacaactcgc atagccactc cgataacggt
    15841 agatacagta aaagaataat aatctaatta catcttgata actcagttga aaatattatt
    15901 caacatacgg cttttttatg gtgagctgac tcacatctcc ctgactctgc ggcaatatgg
    15961 cgtctttagt atctcctgct gaacgccctt tttatccagc ataaaattcc gttcagaagc
    16021 ggattagtgg cactctgata ataagaaatc gcaaagaaaa aaattctccg gcgacgcggt
    16081 ttttaacagg tgttctatgc ttgaaatgag gtgctccaca cagagagcgc cgacaacaat
    16141 gaggtgcgcg tatggcgacg atcgattcta tgaataagga caccacacgt ttgagcgatg
    16201 gacccgactg gacgttcgac ctgctggatg tttatctggc agagatagac cgggtggcga
    16261 aactctaccg gctggatacc tacccgcacc agattgaagt gataacctca gaacagatga
    16321 tggatgccta ttccagcgtc ggcatgccaa ttaactatcc gcactggtca ttcggtaaaa
    16381 agtttatcga gactgaacgg ctgtataagc acggtcagca agggctggcc tatgaaatcg
    16441 tcattaactc taacccgtgt atcgcttact tgatggaaga gaacaccatt actatgcagg
    16501 cgctggtgat ggcacacgcc tgctatggac ataactcttt ctttaaaaac aattgcttat
    16561 tccgcagttg gaccgacgcc agttcgattg tcgattatct gatttttgcc cgtaaatata
    16621 ttaccgagtg cgaagaacgt tatggcgttg atgaagtaga acggcttctg gactcgtgcc
    16681 acgcgctgat gaactacggc gtggaccggt acaaacgccc gcaaaaaatc tcgctgcaag
    16741 aagagaaagc ccggcagaaa agtcgcgaag agtatctaca aagtcaggtc aatatgctct
    16801 ggcgtaccct gccgaagcgc gaggaagaga aaacggttgc tgaagcgcgc cgctatccgt
    16861 ccgaaccaca agaaaacctg ctctatttta tggagaaaaa tgcgccactg ctggaatcat
    16921 ggcagcgtga aatcctgcgt attgtgcgta aggtgagcca gtatttttat ccgcaaaaac
    16981 agactcaggt gatgaacgaa ggctgtgcga ccttctggca ctataccatc cttaaccatc
    17041 tgtatgatga agggaaagta acggaacgtt ttatgctgga gtttttgcac agccacacca
    17101 atgtggtctt ccagcccccc tataacagcc cgtggtacag cggcatcaac ccgtatgccc
    17161 tcgggttcgc catgttccag gatattaaac ggatttgtca gtcgccaacg gaagaagaca
    17221 aatactggtt cccggatatc gccggttccg actggctgga aacgctgcat ttcgcgatgc
    17281 gtgatttcaa agatgagagt tttatcagcc agtttctgtc accgaaagtg atgcgtgatt
    17341 tccgcttctt caccgtgctg gatgatgatc ggcataatta tctggagatt tccgctattc
    17401 ataatgaaga aggttatcgg gagatccgta accggttatc gtcgcaatat aacttaagta
    17461 atctggagcc gaatattcag atctggaacg tggatttgcg cggcgaccgt tcgctgacgc
    17521 tgcgttatat tccacataat cgcgcaccgc tggatcgggg gcgcaaagaa gtcctgaagc
    17581 atgtgcatcg cctgtgggga tttgatgtga tgctcgaaca gcaaaacgaa gacggcagca
    17641 tcgagttgct ggaacgttgc ccgccaagaa tgggaaatct gtaaaaacaa caaaaaaccc
    17701 ctcgtttgag ggggttgctc tttaaacgga agggattatc gcccctgaat ggctaaatca
    17761 cccggcagat ttttctgcat ccggtgccaa atctcgccac tctcatgccc atagcgacgc
    17821 actgtttcgt acacctgatc gtgcgcgcct tcactgcaca acgccgacag tttgtggtag
    17881 aagcccagcg ccagactgcg cgcttccgga ttggcgaaat agtgacgacc aatgcgcgta
    17941 tacagccctt tcatcccgtt aaggatcaga ccgtaaatcg ggttgccgga agcaaacgcc
    18001 aggccgcgga atatgttgta atccagctcg gcaaaggcat cggcgtgatc ggccacttca
    18061 ttagcggtag ccagcacttc ctgcgcttta tcgggatgct gacgaaacgc ggtgcgaata
    18121 aaaatagtgg aaatattggt acgcaccgac agcaaattat caataagctg cggcacactt
    18181 tcgtgatcca gtcgcgccag tgtttcaagg atatttaaac cggaagtttc ccagaaatta
    18241 ttcaccttcg tcggcttgcc atgttgaatg gtcaaccagc catctcgtgc cagacgctgt
    18301 aacacttcac gtaacgtagt acgcgttacg ccaattaatt ctgaaagttc acgttctgcg
    18361 ggcaaaatag tcccgggagg gaagcggtta ttccagatac tttcaataat gtactcttcc
    18421 gcgaaacccg ccgggctttg cgccttaatg accatagtga gatttccata acacagcaaa
    18481 acaaagttgg actcatcata ccagagggct aactacgctg atagcagaca aaaagaagaa
    18541 aaagggatca aggcttcatt ttccgcatct ctttcccctt gactatcaag acttgcctca
    18601 ctcctgcctg accgttaagc tttgcgttta aacataaaaa catcattgat tatttgtaag
    18661 gtaagggaaa ccatcatgga gatctcctgg ggccgcgcgc tatggcgcaa ttttttgggc
    18721 cagtcccccg actggtacaa actcgccctc atcattttct taatcgtaaa cccgttaatt
    18781 ttcctcatca gccctttcgt cgcgggctgg ttgctggtag cggaatttat tttcactctg
    18841 gcgatggccc tgaaatgcta cccgctactc cccggtggtc tgttggctat cgaagcggta
    18901 ttcatcggca tgaccagcgc ggaacacgtc cgtgaagagg tggcggcaaa tcttgaagtc
    18961 ttgctgttat tgatgtttat ggtggcgggt atctatttta tgaaacagct gttgctgttc
    19021 atatttaccc gtttgctgtt aagcattcgc tccaaaatgc tgctgtcgct ctctttttgc
    19081 gtggcggcag cgttcctctc cgcgttcctc gatgccttaa ccgtcgtggc ggtggtgatc
    19141 agcgttgcag tcggttttta tggtatttat catcgcgtag cctcttcccg taccgaagac
    19201 accgacctgc aagacgatag tcatatcgac aagcattaca acgtggttct ggaacagttc
    19261 cgtggctttc tgcgtagcct gatgatgcat gccggtgtcg gcaccgcatt aggcggcgta
    19321 atgaccatgg tgggcgaacc acagaacctg atcatcgcta aagcggctgg ctggcatttt
    19381 ggcgatttct tcctgcgcat gtcgccggtg accgttccgg ttctgatttg tggcctgtta
    19441 acctgcctgc tggtagagaa gctgcgttgg tttggctacg gtgaaacgct gccggagaaa
    19501 gtccgcgaag tgttgcaaca gtttgacgat caaagccgcc accagcgtac ccgtcaggat
    19561 aaaatccgtc tgattgtcca ggcgattatt ggcgtctggc tggtgactgc gctggcgttg
    19621 catctggcgg aagttggctt gattggtttg tcagtcatta ttctggcaac atcattgacc
    19681 ggtgtcaccg atgaacacgc tatcggtaaa gccttcaccg aatctctgcc attcaccgca
    19741 ctgttgacgg tgtttttctc ggtagtcgcg gtgattatcg accaacaact gttttcgccg
    19801 attattcagt ttgtgttgca ggcatctgaa catgctcagc tgtcgctgtt ctatattttc
    19861 aacggtctgc tgtcatccat ttcggataac gtcttcgtgg ggacgattta tatcaacgaa
    19921 gcgaaagcgg caatggaaag tggcgctatc acgttgaagc aatacgagct gctggcggtc
    19981 gccattaata ccggtaccaa tctgccctct gtcgctacgc cgaacggtca ggctgcgttc
    20041 ctgttcctgc tgacctctgc actcgcgcca ttgattcgcc tctcttatgg ccgcatggtg
    20101 tggatggccc tgccttacac cctcgtactg acactcgtcg gcttgctctg cgtcgagttt
    20161 acgcttgccc ctgtaaccga atggtttatg caaatgggct ggatagcaac gctttgataa
    20221 caacttaccg ggcatataaa tgcccggttt gccttttcgc ccgataattg tccaattgcg
    20281 tcctattttt gctgcctcct ggtggcgcag cgaatgaatt ggtttaaact gcgcactcta
    20341 tgcatattgc agggaaatga ttatgttgcg atttttgaac caatgttcac aaggccgggg
    20401 cgcgtggctg ttgatggcgt ttactgctct ggcactggaa ctgacggcgc tgtggttcca
    20461 gcatgtgatg ttactgaaac cttgcgtgct ctgtatttat gaacgctgcg cgttattcgg
    20521 cgttctgggt gctgcgctga ttggcgcgat cgccccgaaa actccgctgc gttatgtagc
    20581 gatggttatc tggttgtata gtgcgttccg cggtgtgcag ttaacttacg agcacaccat
    20641 gcttcagctc tatccttcgc catttgccac ctgtgatttt atggttcgtt tcccggaatg
    20701 gctgccgctg gataaatggg tgccgcaagt gtttgtcgcc tctggcgatt gcgccgagcg
    20761 tcagtgggat tttttaggta tggaaatgcc gcagtggctg ctcggtattt ttatcgctta
    20821 cctgattgtc gcggtgctgg tggtgatttc ccagccgttt aaagcgaaaa aacgtgatct
    20881 gttcggtcgc taatccattc ggcgctcctg cgggagcgct tttttcctgc cgctatattt
    20941 atttgacccg cagtaaatca gaacttcgcg ttgtataacg tggtgaaagc atagctcgct
    21001 tcatctgcca ttgttgttgg atcccctgcc cggcaaaata gagtgttcct ctgccctctt
    21061 tagcattcag agtatccatt accgccatca attgctcact cccggggcgc ggtgcgttgt
    21121 catcgaataa attgagctgc gcgactccct gactgaagaa atcccccagc atcacgcccg
    21181 ctttttggta gcgatggccc gcttgccaga tggcatccag agatcgcgta gcagcgttaa
    21241 tgatatccct gctgtcctga gtgggcgtca gcagttttac cgacgcgcta ttgccgtaat
    21301 aaggttcatt gagcgcaaat ggtgacgtct taataaatgt ggagataaac cgacaatatt
    21361 gatgctcact gcgaagtttt tccgccgccc gggcagcgta actacaaatg gcctgccgca
    21421 tcgacgtata atccgtgatg cgttcaccaa acgagcggga acagataatt tcctgcttcg
    21481 tcggtgcaaa ctcttccagt tgcaaacagg gttcgccgcg cagttcacgc accgttcttt
    21541 cgagcatgac attaaaatgt ttacggataa accggatatc tgtatccgcc aaatcgagaa
    21601 cggttttgat ccccatcgcg tccagttttt tgctgatccg ccgtccaatc ccccagacgt
    21661 catccacggg gagagcagac attaatttac gctggcgttc cagatttgat aaatccacca
    21721 ccccacccgt ctgccgctgc catttttttg ccgcatgatt ggcaagctta gccaacgttt
    21781 tggtctgggc gatccccaca ccaacagtaa gatgggtacg ttgtagcacc gttgcgcgaa
    21841 tttctctgcc aaaatcagtc agatcgcgac aattacgcac acctgtcaga tcgcagaatg
    21901 cttcatcaat actgtaaatc tcgacgcggg gcgatagttc ttccagcgtc gacatcaccc
    21961 gattgctcat gtctgcgtaa agctcataat tgctgctaaa gcaaaccaca ccacagcgac
    22021 gaaacagatc tttttgtttg aaccagggat cgcccatttt aacgcccagc gcctttgcct
    22081 cagcgtttcg ggcgataacg caaccgtcat tattcgatag cacaaccacc ggtttacccc
    22141 ataaatcagg gcgaaacacc gtctcacagc tggcataaaa cgcgtttaca tcacagaggg
    22201 caaacatcag cgcatcgcct taacgacgtg gatcaccaca ccaaagacat ccagcgtatc
    22261 ttcgctactg atggtaatgg gcgagtacgc actgttcatg ggaataagct gtaccgtcgg
    22321 gcgtagttgc aattttttca ccgtaaactc gccgtcaaca gcagcgatga caatatcacc
    22381 atggctggcg gtaatagcac tatcgacaat cagtaaatca ccgtcactaa ttccaccatc
    22441 aatcatagaa tcaccacttg ctttgacgaa gtaagtcgcg ctgggatgct ggatcaacag
    22501 ttgattcaga tcgatgcgct gttcaacgta atctgctgcc ggtgaaggaa agccacactg
    22561 aacaagatcg ctaaatagcg gaaaagtcac aatttcgcgg agatccgcag gcttgataaa
    22621 caacataata atctgcctga agttatactg tttttatata cagtggtctg ttcttgccag
    22681 cagatcaata ctgattcagg ctatcaatat ttgtcgctgc atagactgct gatttttcgt
    22741 tctcttatcc tgtgctcacg tggccttctg gcgacgacgc tcatccagca gaaatgaaaa
    22801 atatcacccg gcaaaaaaat agaatagaag cattcgccat aatgacatta aacattgttt
    22861 gatatttatc atattaatag aaataaagac a
//
LOCUS       DAZPCS010000049        22278 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZPCS010000049 DAZPCS010000000
VERSION     DAZPCS010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22278
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.049"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..1025)
                     /locus_tag="ACHUQ7_001899"
     CDS             complement(<1..1025)
                     /locus_tag="ACHUQ7_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1135332.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEA"
     gene            complement(1127..2107)
                     /locus_tag="ACHUQ7_001900"
     CDS             complement(1127..2107)
                     /locus_tag="ACHUQ7_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFR1135333.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGATSVRFYPCYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYASAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(2115..2825)
                     /locus_tag="ACHUQ7_001901"
     CDS             complement(2115..2825)
                     /locus_tag="ACHUQ7_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFR1135334.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(3042..4865)
                     /gene="typA"
                     /locus_tag="ACHUQ7_001902"
     CDS             complement(3042..4865)
                     /gene="typA"
                     /locus_tag="ACHUQ7_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFR1135335.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            5238..6647
                     /gene="glnA"
                     /locus_tag="ACHUQ7_001903"
     CDS             5238..6647
                     /gene="glnA"
                     /locus_tag="ACHUQ7_001903"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFR1135336.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            6820..7869
                     /gene="glnL"
                     /locus_tag="ACHUQ7_001904"
     CDS             6820..7869
                     /gene="glnL"
                     /locus_tag="ACHUQ7_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFR1135337.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            7881..9290
                     /gene="glnG"
                     /locus_tag="ACHUQ7_001905"
     CDS             7881..9290
                     /gene="glnG"
                     /locus_tag="ACHUQ7_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFR1135338.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            9402..9512
                     /locus_tag="ACHUQ7_001906"
     CDS             9402..9512
                     /locus_tag="ACHUQ7_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFR1135339.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(9741..11114)
                     /gene="hemN"
                     /locus_tag="ACHUQ7_001907"
     CDS             complement(9741..11114)
                     /gene="hemN"
                     /locus_tag="ACHUQ7_001907"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFR1135340.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVYEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(11303..11812)
                     /gene="yihI"
                     /locus_tag="ACHUQ7_001908"
     CDS             complement(11303..11812)
                     /gene="yihI"
                     /locus_tag="ACHUQ7_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFR1135341.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDECLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            complement(11931..12097)
                     /locus_tag="ACHUQ7_001909"
                     /pseudo
     CDS             complement(11931..12097)
                     /locus_tag="ACHUQ7_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12394..13026
                     /gene="yihA"
                     /locus_tag="ACHUQ7_001910"
     CDS             12394..13026
                     /gene="yihA"
                     /locus_tag="ACHUQ7_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFR1135342.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKALLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(13408..16194)
                     /gene="polA"
                     /locus_tag="ACHUQ7_001911"
     CDS             complement(13408..16194)
                     /gene="polA"
                     /locus_tag="ACHUQ7_001911"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFR1135343.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEVGKWLQAKGAKTAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKELGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            16558..17490
                     /locus_tag="ACHUQ7_001912"
     CDS             16558..17490
                     /locus_tag="ACHUQ7_001912"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFR1135344.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGQDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(17531..18961)
                     /locus_tag="ACHUQ7_001913"
     CDS             complement(17531..18961)
                     /locus_tag="ACHUQ7_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFR1135345.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLAYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(19116..19742)
                     /gene="dsbA"
                     /locus_tag="ACHUQ7_001914"
     CDS             complement(19116..19742)
                     /gene="dsbA"
                     /locus_tag="ACHUQ7_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFR1135346.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEEK"
     gene            complement(19759..20745)
                     /gene="srkA"
                     /locus_tag="ACHUQ7_001915"
     CDS             complement(19759..20745)
                     /gene="srkA"
                     /locus_tag="ACHUQ7_001915"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFR1135347.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFKQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAQQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(20822..21091)
                     /locus_tag="ACHUQ7_001916"
     CDS             complement(20822..21091)
                     /locus_tag="ACHUQ7_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFR1135348.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            21161..21745
                     /gene="mobA"
                     /locus_tag="ACHUQ7_001917"
     CDS             21161..21745
                     /gene="mobA"
                     /locus_tag="ACHUQ7_001917"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFR1135349.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            21727..22254
                     /gene="mobB"
                     /locus_tag="ACHUQ7_001918"
     CDS             21727..22254
                     /gene="mobB"
                     /locus_tag="ACHUQ7_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFR1135350.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAVQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         5515 a         5584 c         5333 g         5846 t
ORIGIN      
        1 gcttcgccga taggtgcaaa aaatacggct cgctgtgtaa agactatcgc gccaaagccc
       61 agcgtacatg ccatccctaa atagaccggc atactttcgt gcggcagcat aatcaacagt
      121 acgagcgcag cggtactgat gataaaggtg tagcatagat atttactcgg cgatttcagg
      181 atcttatctg aaatcatgcc accaatcggt ccaccaatca ttttcagaca gtattggtta
      241 atgatgccgt aagcccccac cagcgcaacg ggcaatagat agatgttttt caggaatgga
      301 atgaagaatg tcaggccaca gtaaaccgcg taaacgaaga agacgttgaa agcgataagc
      361 cagatggttt tgtctctcag cactgaggtc atactggtgt tttgcgatgc tccgtcttct
      421 tttttcacct caacggacgg tgcctcttct ttgtcattca ggacaaagaa aataataatg
      481 cctacggcaa tcacaataag ggaatagaac cagatgcctg ctttaaaacc taataagcca
      541 ctgccaaacc aggtaaatac tgccaacgca gaaaatgcca ccacggtatc gacaatgcca
      601 cgccctgttt cgaagaagcc aaacaaccga ccttgttgtt cgctattgcc caatcgactt
      661 accgacttga gcaagaccgg ccagttcatc atgtcgcaag taacgccaaa tagcgcccag
      721 acaaagagga tgccccaata ccccggcatt gtcgtcaaat aaacacccag taatcctgtc
      781 gcaatgagtg agaatgacat ggtgtatctg cgcggtagtt tatcggcaaa ataaatagac
      841 agaaaaaagc ccactgtggt gacaaatgag tttaccgaca tagcattacc aatttgacca
      901 ttggtcaaat ggaaatattc ctgcatcggg atataaaacg catctttcag cgacggtaat
      961 ttataaattg taccgccaca cagtgttaat agactaaata acgcccattt ctttttcgtg
     1021 agcataacat cccctaagcg atttttaaaa tcactgtttt aaaggcaata agataccgac
     1081 cattcatatg gaatggtttt attaactgcg tatgagttaa aacttattag tggttcagaa
     1141 gatggtttgc ctcttgtttg atttgttgta gtacgttacg cacatgattt atctgattaa
     1201 ttgcatcttc tttttcttta cgtaaccgcg catccagtaa atgattttct ggtaggggtg
     1261 tttcactcat ctggcgataa gggatccacg gattatcatc ttcgtcttca aagcggaacg
     1321 ccgacgcata gtaatcaacc tcttcttcca ggccatatgc cgtcacctgc ggctcatcat
     1381 cgccaaggca gataaggtgc gttaataacg ctttgaatgg catatcaccc tgacctgaaa
     1441 tacaggcttt atgacccagg ccaccctgtt ctttaacgat caaggcatct ttaatatgga
     1501 cctgggtaat atgcggtgcc atcgttttta aggcgtcgat gggatgctca tttgcattga
     1561 tcatgttagc aaaatcaaac agtaaggaaa gagactccat ctcactttct ttgaccagcg
     1621 acaccagctc atgacttttt aaatcttcat gctgctcaat cgtaaaagtc aggccgctgt
     1681 cctgatacgt ttcccgtaca taggcaatgt cgttagcgat aatcgataat acgtcgcgca
     1741 aattaccttc ataacatgga taaaaacgta cggacgttgc cccagttttc aacgcgatgg
     1801 cgacggcttc gtcaatagat gccttatctg aagcgctggt ttcaatatga atatcaaggt
     1861 tcagacggcg ggctttatcc ccaaaggcag agagttcttt atcgtccata ttaccaagag
     1921 aaaaacgctc gccatccaga acatggattt tgacgccacg tagattattt tcactggcga
     1981 tatcaagtaa atcagcaggc aacactcttt ccagccgcat atttaaatga aaggcataag
     2041 catgtaaata aagaggaaga gtgtcgacac gttgtagaat ctttcttgcg ttatttattg
     2101 tcaccataaa acacctacag attaatgatg aggtctacgg cctcaaatcg ccaatattca
     2161 atatctatct ggacaatatt gccttgctgg tcggcgcgat gcttttcaat taagatcgct
     2221 ggcatacccg cagaaccgcc aagatacttg ctgatatcgc ccggcattct gacgggtttg
     2281 aaaatcaatt tttttactac cgtctctttt tggtagcact tttcccagac tgcagaaaat
     2341 gagtggtttt caagttgttc aataaaaccc ggagcaactt caggattaat atatgtttcg
     2401 tgatagaaaa ctttatgtcc ttccagcgcc ccccagccgg tgatgcgata gagttcagtt
     2461 gatggcgtca ctgcaatcag tggcgcgagc gtctcgggaa tatcgctggt acggtttttc
     2521 tcggtaaacc cccaagaagg ctctcgccct tgctcaattg cggcccgctg aaagctcgcc
     2581 gacagctccg gactgtaatt aaatcgcggc tgggtcacga accacccctt acgatcctta
     2641 cggaagattt tggattctgc ctcgaggttc aacaacgcct ggcgcagcgt catacgttta
     2701 atgcccagca gttctcccag ctctctttct gaagggagtt ttccacccgg cgtggtgatg
     2761 ccatctttca accaccgatc cagtttctct tttgcatttt ctacggtgga ttgattctcc
     2821 gccattgttt aatccttttg gtttaaacca gatgaagcat cttacgctta cgccaaaaaa
     2881 gaaagcaatt aacggtgtat gactttgcag ataattgtga caaagctctc tattgacgta
     2941 atcacagact tataagggag atactcgcat tggtgtgcgc tcacgacagg taaaaaaaaa
     3001 cctgccatcg ctggcaggtt ttttatgact aaaaaacgaa attaatcgtc tttcggtgcg
     3061 cggttggcgc ggcgacgatc gttttccgtc aggtgacgtt tacgaatacg gatagaggtc
     3121 ggagtcactt ctaccagttc gtcatcatcg atgaactcca gagcttgttc cagagtcatg
     3181 cggataggcg gaaccagaac aacagcttcg tcagtaccgg aagcacgcat gttggtcagt
     3241 ttcttaccgg tcaggcagtt tacagtcagg tcgttagagc ggctatgaat accgataatc
     3301 tgaccttcgt aaacttctgc accgtgaccg aggaacagct taccgcgatc ctgcagaccg
     3361 aacagcgcga acgcgacagc tttaccctga ccgttagaga tcagtacacc gttctgacgc
     3421 tgacccactt cgcccggacg tacgtcgtcg tagtggctga aggtggagta cagcagacca
     3481 gtaccggaag tcatggtcat gaactcagaa cggaagccaa tcagaccacg gcttgggatc
     3541 acgtagtcga gacgtacgcg gcctttaccg tctggattca tgtttttcag gtcgccttta
     3601 cgttcgccca gcgcctgcat cacagaaccc tgatgctgtt cttcaacgtc cagcgtcacg
     3661 ttttcatacg gctcttgttt acgaccgtct atttcacgga agattacttt cggacgggat
     3721 accgccagtt cgaaaccttc acgacgcatg ttttcgataa gaacagagag gtgcagttca
     3781 ccacgaccag aaacgcggaa cgcatcggcg tcttcggttt cttctacgcg cagcgcaacg
     3841 ttgtgtacca gttctttgtt cagacgatcg aggatctgac gagacgttac gaacttacct
     3901 tctttaccgc agaacggcga ggtgttaacg cagaagaaca tagaaacggt cggctcatca
     3961 acggagagtg ccggcagcgc ttcaacgttt tgcgtgtcgc aaacggtgtc agaaatgttc
     4021 agttcgccca ggcccgtgat cgccacgata tcgccagctt ccgccagatc ggtttcgata
     4081 cgttccagac cgaggtggcc cagcacttta ccgactttcg cgttacgggt tttgccttcg
     4141 ctatcgatga tagtaacctg ctggttcggc ttcactttac cacgcttgat gcggccaatg
     4201 ccgataacgc caacatagct gttgtaatcg agctgagaaa tctgcatctg gaacggaccg
     4261 tcaaggtcaa cgtccggcgc aggaacgtgg tcaacaatcg cctgatacag cggggtcatg
     4321 tcttccgcca tatcttcgtg gtccagaccc gcgataccgt tcagcgcaga agcgtaaacg
     4381 atcgggaagt ccagctgctc gtcggtcgcg tcgaggttaa cgaacagatc gaatacctga
     4441 tccacaaccc aatcaggacg cgcgccaggg cggtcaactt tgttgataac aacaatcggc
     4501 ttcaggccgt aagcaaacgc ttttttggtt acgaagcgcg tttgcggcat cgggccgtca
     4561 aatgcgtcaa ccaccagcag cactgagtct accatggaca ttacacgttc aacttcacca
     4621 ccgaagtcgg cgtgccccgg ggtatcaacg atgttgatac ggtaatcatt ccatttgata
     4681 gcggtgtttt tcgcgaggat ggtaatccca cgctctttct ccaaatcgtt ggagtccatc
     4741 acgcgctctt gggtttcggc acgagagtcg aacgtaccgg attgttggag cagcttgtct
     4801 accagggtgg ttttaccatg gtctacgtgc gcgatgatgg cgatattacg caatttttcg
     4861 atcacaactt tgcctcaggc attagaaata gcgcgttatt gtacacggat taatcgcact
     4921 acaaaacagg atcacaaaca tcctccgcaa acaagtattg cagagtccct ttgtgatcgc
     4981 tttcacggag cataaaaagg gttatccaaa ggtcattgca ccaatatggt gcttaatgtt
     5041 tccattgaag cactatattg gtgcaacatt cacatcatgg tgcagccctt ttgcacgatg
     5101 ttaagcatga taacgccttt taggggcaat ttaaaagttg gcacagattt cgctttatct
     5161 tttttacggc gacacggcca aaataattgc agatttcgtt accacgacga caatgaccaa
     5221 tccaggagag ttaaagtatg tccgctgaac acgtactgac gatgctgaac gagcacgaag
     5281 tgaagtttgt tgatttgcgc ttcaccgata ccaaaggtaa agaacaacac gtcactatcc
     5341 ctgctcatca ggtgaatgct gaattcttcg aagaaggcaa aatgtttgac ggctcctcga
     5401 ttggcggctg gaaaggcatt aacgaatccg acatggtgct gatgccagac gcatccactg
     5461 cagtgattga cccgttcttc gccgactcca ccctgattat ccgttgcgac atccttgaac
     5521 ctggcaccct gcaaggctat gaccgtgacc cgcgctctat tgcgaagcgc gccgaagatt
     5581 acctgcgttc cactggcatt gccgacaccg tactgttcgg gccagaacct gaattcttcc
     5641 tgttcgatga catccgtttc ggatcatcta tctccggttc ccacgttgct atcgacgata
     5701 tcgaaggcgc atggaactcc tccacccaat acgaaggtgg taacaaaggt caccgtccgg
     5761 cagtgaaagg cggttacttc ccggttccgc cggtagactc tgctcaggat attcgttctg
     5821 aaatgtgtct ggtgatggaa cagatgggtc tggtggttga agcccatcac cacgaagtag
     5881 cgactgctgg tcagaacgaa gtggctaccc gcttcaatac catgaccaaa aaagctgacg
     5941 aaattcagat ctacaaatat gtcgtgcata acgtagcgca ccgcttcggt aaaaccgcga
     6001 cctttatgcc aaaaccgatg ttcggtgata acggctccgg tatgcactgc cacatgtctc
     6061 tgtctaaaaa cggcgttaac ctgttcgcag gcgacaaata cgcaggtctg tctgagcagg
     6121 cgctgtacta cattggcggc gtaatcaaac acgctaaagc gattaacgcc ctggcaaacc
     6181 cgaccaccaa ctcttataag cgtctggtcc cgggctacga agcaccggta atgctggctt
     6241 actctgcgcg taaccgttct gcgtctatcc gtattccggt ggtttcttct ccgaaagcac
     6301 gtcgtatcga agtacgtttc ccagatccgg cagctaaccc gtacctgtgc tttgctgccc
     6361 tgctgatggc cggtcttgat ggtatcaaga acaagatcca tccgggcgaa gccatggaca
     6421 aaaacctgta tgacctgccg ccagaagaag cgaaagagat cccacaggtt gcaggctctc
     6481 tggaagaagc actgaacgaa ctggatctgg accgcgagtt cctgaaagcc ggtggcgtgt
     6541 tcacggatga agcgatcgat gcgtacatcg ctctgcgtcg cgaagaagat gaccgcgtgc
     6601 gtatgactcc gcatccggta gagtttgagc tgtactacag cgtctaatag ttgaagttgt
     6661 actacccggc gcacaacgcc gggatttagt tgccgtggaa actttcagcc catctctgca
     6721 tgggcttttt tctccgtcaa ttctctgatg cttcgcgctt tttatccgta aaaagctata
     6781 atgcactaaa atggtgcaac ctgttcagga gactgcttta tggcaacagg cacgcagccc
     6841 gatgctgggc agatcctcaa ctcgctgatt aacagtattt tgttaattga tgacaatctg
     6901 gcgatccatt acgccaaccc cgccgcgcaa caactgctcg cccaaagctc ccgcaaattg
     6961 tttggtacgc cgttaccgga actgttgagc tacttctcat taaatatcga gctgatgcaa
     7021 gaaagtctgg aggcggggca aggctttacc gataacgaag tgacgctggt catcgatggt
     7081 cgctcgcata tcctttctgt gacggcccag cgtatgccgg acggcatgat cctgctggag
     7141 atggcaccga tggataacca gcgccgctta agccaggaac aactacaaca tgctcaacaa
     7201 gtagctgccc gtgatttagt gcgcggcctg gcacatgaga ttaaaaatcc gcttggcggt
     7261 ttacgtggcg cggcgcagtt actcagcaaa gcgttacctg acccatcact actcgaatat
     7321 accaaagtga ttatcgaaca ggcggaccgg ctgcgaaatt tggtcgaccg tctgttgggg
     7381 ccgcagctgc ccggtacgcg cgttaccgaa agtattcaca aagtggctga acgcgtggta
     7441 acgctggtgt cgatggaact gccggacaac gtgcggttga ttcgtgatta cgatcccagc
     7501 ctgccggaac tggcgcacga cccggatcaa attgaacagg tcttgctgaa tattgtgcgc
     7561 aatgcgctac aggcgctggg gccggaaggc ggtgaaatca ttctgcgtac ccgcaccgcg
     7621 tttcaactga ccttacacgg cgagcgctac cggctggcgg cgcggattga tgtggaagat
     7681 aacgggccgg gcattccgcc tcatttgcag gatacgctgt tttacccgat ggtcagcggc
     7741 cgcgaaggtg gcaccgggct tggcttatcc atcgcccgta atttgattga tcagcattca
     7801 ggcaaaattg aatttaccag ttggccaggt cataccgagt tctcggttta cctgcctatc
     7861 aggaaataaa ggtgacgttt atgcaacgag ggatagtctg ggtagtcgat gacgatagtt
     7921 ccatccgttg ggtgcttgaa cgtgcgctcg ctggagcggg tttaacctgt acgacatttg
     7981 agaacggcgc ggaagtactg gaggcgctgg cgagcaaaac gccggatgtg ctgctttcag
     8041 atatccgtat gccgggaatg gacgggctgg cgctgctcaa gcagattaaa cagcgccatc
     8101 caatgcttcc ggtcatcatt atgaccgcac attccgatct ggatgctgcc gtcagcgcct
     8161 atcaacaagg ggcgtttgat tatctgccca aaccgtttga tatcgacgaa gcagtggcgc
     8221 tggttgagcg cgctatcagt cattaccagg aacagcagca gccgcgtaat attcagctta
     8281 acggcccaac gaccgatatc atcggcgaag cgcaagccat gcaggacgtg ttccgtatta
     8341 tcggtcggct ttcgcgttct tctattagcg tgctgattaa cggcgaatcc ggcaccggta
     8401 aagaactggt cgctcatgcc ctgcatcgcc acagtccgcg caccaaagcg ccgtttatcg
     8461 cgctgaatat ggcagctatc ccaaaagatt tgatcgaatc agaactgttt ggccacgaga
     8521 aaggcgcgtt tactggcgcg aataccattc gtcaggggcg ttttgaacag gccgatggcg
     8581 gcacgttatt tctcgacgaa attggtgata tgccgctgga tgtgcagacg cgtttgctgc
     8641 gcgtgctggc agacggtcag ttttatcgcg ttggcggcta tgcgccggtg aaagtggatg
     8701 tgcggattat cgctgccact caccagaatc ttgaacagcg ggtgcaggaa ggtaagttcc
     8761 gtgaggatct gttccaccgc ctgaacgtta tccgcgttca tctgccgccg ctgcgcgaac
     8821 gtcgggaaga tattccccgt ctggcgcgcc attttttaca ggttgccgcg cgcgaactgg
     8881 gcgtagaagc gaagttgctg catccggaaa ccgaagctgc tctgacgcgt ctggcgtggc
     8941 caggcaacgt gcgccagctg gaaaacacct gccgctggct aacggtgatg gccgccgggc
     9001 aggaagtgtt gattcaggat ttgcccggcg aactgtttga atcaacggtt gcggagagta
     9061 cttcgcaaat gcaaccggac agttgggcga cacttttagc gcagtgggca gacagagcgc
     9121 tgcgttccgg tcatcaaaat ctgctttccg aagcgcagcc agagctggag cggacgttac
     9181 tgacgaccgc gttgcgacat acgcaggggc ataaacagga agcggcgcgg ctactcggct
     9241 ggggccgcaa cactctgacg cgtaagttaa aagagctggg gatggagtga ttcacggctt
     9301 gtgtgtaaag attgattatt gagcgcaaat tgctggtatt ttgcgcttta ctgttccgat
     9361 aagttcagta tgatcttgcc cggaaaacgg ggagagtcat tatgctggaa tcaataatta
     9421 atctggtatc gagtggcgca gttgacagcc acacaccgca aactgccgtt gctgccgtgc
     9481 tgtgtgccgc gatgattggg ctgtttagct gaagctcgtt gtttatgccg gatgcggaga
     9541 aagtcgtagg cctgataaga cgcgaccagc gtcgcatcag gcatcatgct cagatgcctg
     9601 atgcggcgta aacgccttat ccggcctaca aaagcaataa tttcaacagg ttttgttgcc
     9661 cagcgcatca ggcaatcttg ttctcagcac attgatggcg ctttcgtttt ctactttgta
     9721 aacgaagcgc cattcactct ttaaatcacc cgtgagaact gctgcatccg cgctttctgg
     9781 cgcagatagg tatcaaagca catgcaaatg ttgcggatca gcaagcgacc tttcgccgtc
     9841 acctgtattc ccttctcatc cacatccacc agcccatctt ttgctaacgg ggcgagcagc
     9901 ttgagatctt ccgcaaagta atcagcgaag tgcaaatccc actgtttctc aagaggggcg
     9961 taatccagac ggaagttgca gatgagcgac ttaatcacat cgcggcgaat acagtcatca
    10021 cgcgttagcg caataccacg ccacagcgca ttgccttgtt catacacttg ctgatagtac
    10081 tgcttcaact ctttctggtt ctgcgcgtag cagtcgccaa tcatgctgat ggcggaaacg
    10141 cccatcccca gcagatcggt atcgccctga gtggtgtagc cctggaagtt acgatgcagc
    10201 acgccttcac gctgggccac cgccagctcg tcatcaggac gggcaaagtg atccataccg
    10261 ataaactgat agcccgattg cgtcaggaag gcgatggttt cctgcaggat atcgagtttt
    10321 tgctgcggac tcggcaggtc agcatctttg attttgcgct gagcagcaaa aatggtcggc
    10381 agatgcgcgt agttaaaaac gctcagacga tcggggttca gctccgccac acgtttcagg
    10441 gtaaaggcga aactctccgg cgtctgtttc ggcaggccgt aaatcaggtc gatgttggtg
    10501 gaggtaaaac caatctcacg cgcatggtta agcagtgcaa agatgaactc ttcatcctgc
    10561 tcgcggttaa ccagacgttg cacttctttg ttgaagtcct gcacgcccat gctcaggcga
    10621 ttaaagcctt cggcgcgtaa atgatcgagt acatccagtt cgatttcccg cggatcgact
    10681 tcgatcgaaa tctccgcatc ggtattgaaa tggaagtttt cgcgcagcag cttcatcaga
    10741 cggctgattt gcgctttatt cagatacgtc ggcgttccgc cgccccagtg caattggctg
    10801 acgtgacgcc cggcgaacag cggtgcacga tggacgattt cttgctccag cgcgtccaga
    10861 tactgatcgg ccttgtgctg ctggcgagta acaatcttat tgcaaccgca gaagtaacaa
    10921 agcttatggc agaacgggat atgtacgtag agagataatg gacgctcagg atagcgcgcc
    10981 acggcttgta aaaacgcctg ttcgccgaag tcttctgaaa actccagcgc ggtcgggtac
    11041 gaggtgtatc gtggcccgga atagttatat ttctggatca gggccagatc ccagtcgatt
    11101 tgctgtacag acatgctcac tccttccgat ggcgtctctg gcggctacgg cgtaccggcg
    11161 gatgcccgtt tcgggtcatc agccggttgc gcagccactt ttgtcgccgc gacaaccgct
    11221 gtagtttaac gaataaccac accagataac atataaccgg aagggttatc agcagaacgg
    11281 gaaggcccac ggcggcaatc cgttagttgc cccgcagcag acgcatcatg tcttcttgct
    11341 tctcgtcttc ttcctcttct tcgtcatcgt cataagagag gccgagtttc tgcatcaact
    11401 catcaatacg atccagttta gcatccaccc aggattgctc ttcggcgctc agggtttcgc
    11461 ctgcttccag acgttccagc agcgcatcca gacactcatc cgtttccagt aattccaact
    11521 ccgcctgcgg tgaaagcata ggtttctcac tcttcggttt gtgctgtttg gtgacttttt
    11581 cagtcactcc caatggaata ggggttttac tgccaatacg tggatctttt ggtgcgttct
    11641 ggcctttgct gcctgacgtg gtgtttccgc ccgctgcgcg gctgcccggc gcatgaccac
    11701 gacgtttttt ctgacgctta cggtcacgag cttcctgatc cagctcctcg cgtgtttttc
    11761 gacgcgcttt tgcatgacct ttgctgcgtg agtttgaaga tgatggtttc ataatggttc
    11821 gtcttttagc cgttttattc agtatagatt tgcggcggaa tctaacagaa agcaagcaaa
    11881 gaaaaaaggc gacagattac tctgtcgcct tttttcctga ctcataaccc ttaacgggtc
    11941 ttacaatcct tgcaggccgc caacacaccc tgtttttcca ttagccggaa caatccgtgt
    12001 tgattccatt tccgtttaat tacgtctccg gacgtttgtc ttcctgacaa tcctctgtct
    12061 tcgcctcctg gcgctcctga ccctcatcct gagtcattgt tcctgttagc gtcctcgctc
    12121 tatgggtgct actttacgcc tttgctttaa acaaacaatc aaccgccatc agtatttgca
    12181 cattttcaga gttttatata aaagcaattc actgatttat atagcttatt tgacatgcat
    12241 cgcagaattt tctctgctat ttcgcataaa gactatcggc aatgtctcac aaagcgtatg
    12301 gcttttactt acaacgctca cgccgaaaaa tccgcctttt attatggttc aggtataatc
    12361 gccacaaagc tttgactgac ggagacgacc gctttgacta atttgaatta tcaacagacg
    12421 cattttgtga tgagtgcgcc tgatattcgc cacctacctt ccgataccgg aattgaagtg
    12481 gcttttgcag gccgttccaa cgcaggtaaa tccagcgcgc tgaacacgct gactaaccag
    12541 aaaagcctgg ctcgtacctc aaaaacccca gggcgcaccc agcttatcaa cctgtttgaa
    12601 gtggctgacg gcaagcgtct ggttgacttg cctgggtacg gttatgcgga agtcccggaa
    12661 gagatgaagc gcaaatggca gcgtgcgctc ggcgaatacc tcgaaaaacg tcagagcctg
    12721 caaggtctgg tggtgctaat ggatattcgc catccgctga aagatttgga tcagcagatg
    12781 attgagtggg cggtagacag caatatcgcc gttctggtgc tgctgaccaa agcggacaaa
    12841 ctggcaagcg gcgcacgtaa agcgctattg aatatggtgc gtgaagctgt actggcgttt
    12901 aacggtgatg tgcaggttga aacgttttct tcgttgaaga aacaaggcgt agacaagctg
    12961 aggcagaaac tggatacctg gtttagcgag atgcagcctg tagaagaaac gcaggacggc
    13021 gaataatttt cttgccttaa tgcttgtgcc ggatgtggcg tatccggccc gtaaattcaa
    13081 tagattacaa ttctaccgtt ggcctgataa acatagcgca tcaggcatta cggatctttt
    13141 ctttcgccca ataaaaaacg ccccagtcat tactgactgg ggcggctaaa atattcagcc
    13201 aaatccgatt acgtgaagta aaaggtctga aagatagaac atcttacctc tgtaccctac
    13261 gcgactaact ttactctttt ttgttcagtt cagaaagcac tttttgtaat tttttttcag
    13321 ttgttgcata ggaatttcta atagccatca caaaacgtga cagcttatgt tgcttactta
    13381 cgaaaaaagg gcatgttcag gcgaatctta gtgcgcctga tcccagtttt cgccactccc
    13441 cacttccacc agcaacggca catccagacg ggtacagttt tccatcagtt gatgaatctg
    13501 cttcgcgacg gcatcaacat catctttatg aacttcaaat accagttcat cgtgtacctg
    13561 catgatcata cgtacacgcg gttgctcagc ctgtaaccac gcatcaacgg caatcatcgc
    13621 ccgtttgata atgtcggcgg cggttccctg cattggcgcg ttaatggctg cacgttcagc
    13681 cgctgcacga cgagcaccat tgctggattt gatatccggc agatacagac ggcgtccgtc
    13741 cagcgtttca acgtagccca gctctttcgc ctgagcacgg gtgcgttcca tatactccag
    13801 cacgccaggg tagcgttcga agtaaaggtc catgtacttc tgcgcttctt tacgtggaat
    13861 gttcaattgc cgcgccagac cgaaagcact catgccataa atcagaccaa agttgatcgc
    13921 tttcgcgcta cggcgttgct cgctggtgac ggtttccagt ggcaaaccaa acacttctgc
    13981 cgccgttgcc cggtggatat cttttccttc cgcgaatgcg gtcagcaagc ctttatcacg
    14041 cgagagatgc gccataatgc gcagttcaat ctgcgagtag tccgctgaga caatcacata
    14101 atcctctggc gcaataaacg cctggcggat acgacgacct tcttcgttac gcaccggaat
    14161 gttttgcagg ttaggatcgg ttgacgataa acgtcccgtt gcagttactg cctggtgata
    14221 agaggtatgc acacgcccgg ttttcgggtt gatcatcagc ggcagcttgt cggtgtaagt
    14281 cgacttcagc ttcgccagac cacgatactc cagaatcact tttggcaacg gatagtccag
    14341 cgccagttct tccagtacct cttccgacgt tgacggcgcg ccacccggcg ttttcttcag
    14401 cggtttaata ccctgttttt caaacagaat ggtttgcaac tgcttggtgg aagaaaggtt
    14461 aaattcttca cctgcaattt catgcgcttt cttttccagc tcagccagac gcaaggtgag
    14521 ctcttcagaa tgattgtgca gcactttcgg atcgatcttc acaccgttac gttcaatgcg
    14581 tgaaagcacc ggcaccagcg gcatttcgat attctcgaag acgttcaacg gccctttgtg
    14641 tttctgcaga tccggccaca ttttcagatg caactgcaag gtgacatctg catcttcggc
    14701 ggcgtaacgt ccggcctctt cgagggcaat ctggttaaag gtcagttgat ttttgccttt
    14761 acccgcaatc tcttcaaaag tgatggtttt gtgcttcaac caacgttccg cgaggctgtc
    14821 catatcgtga cgcccggcaa cgctattgag aatgtaggac tccagcatgg tatcaaacgc
    14881 aatcccacgc agttcaatgc cgtaattcgc cagaataccg cgatcgtatt tcaggttttg
    14941 cccgaccttc agcgcctttt catcttccag cagcggtttt agcaactcga gtgcacgctc
    15001 gtgagagatt tgatcgggcg catcaagata atcatgagca accggaatat atgccgctac
    15061 gcctggctcg atagcaaaag aaagcccgac caggttagca gagatgttat caaggctgtc
    15121 ggtttcggta tcaaatgcaa ataccggcgc tttttccagc ttcgcaatcc acgctttcag
    15181 tgtttcttca tcaaggatag tgacgtagtt gtcataagaa atcaccgttg ccgtcacttc
    15241 tggcgcttcg tctgcaacac tggtttcctg aggcttcgcg gctgtttttg cccctttggc
    15301 ctgtaaccat ttgcccactt cgacatcagc agtccagcgt ttgaactcat actttttgaa
    15361 cagccccaac aactcttccg ctgccggttg ctgcacttcc agttgttcac aggtcagctc
    15421 cagttcaaca tcggttttaa tcgtcgccaa ctggtatgag agataagcaa cttctttgtt
    15481 ttgctcgagc ttcgctgcca ttgttttcgc gccacggaag ctcaacccag caattttttc
    15541 tggctcggca tacagcgtat ccagcccgcc aagaccttgc agcaatgcct gcgcggtttt
    15601 ttcaccgacg cccggtacgc caggaatgtt atcagaggag tcacccatca gcgccaggaa
    15661 gtcgatgatc aattctggcg gcacgccgta cttattcacc acctcttccg gtccgaggat
    15721 ggtattcgtc atggtattga taagcgtaat atttggcgtc accagctgcg ccatatcttt
    15781 atcgccagtg ctaatcagca ccggacgccc ggctttttcg gcttcgcgcg ccagagtacc
    15841 gataacgtcg tccgcttcta cgccagaaac cgccagcagc ggcagtccca tcgctttaac
    15901 catcgcgtgc agaggttcga tttgtgctcg cagatcgtcc ggcattggcg ggcgatgtga
    15961 tttgtaatgt tcaaacagtt catcacgaaa ggtttttccc ttggcgtcaa agaccaccgc
    16021 tgcatgcgtc ggtttatatt gcatgatcag actgcgcagc atgttgagga caccatacat
    16081 cgcaccggtc ggctcgcctg cgctgttagt cagcggggga aacgcgtgat atgcgcgata
    16141 aagataagat gaaccatcta caaggataag tggattttgg gggatctgaa ccataatgtc
    16201 cgtgcctgtt tatcagatta tgggtaaagg atgccacaga caggatgaaa acatgagttt
    16261 ttcaccgctt ttcgtgaaaa tcttttgtag atctgctgga tcgctcgcaa atcgtcatgt
    16321 ggataacttt gtgcataaat tatttacatc gcttttaatt tattatcatg ctttttacag
    16381 catgatgttt gtctttgttt ttcatgcagt taaaaataaa tccatcccta ataattgaga
    16441 taatgggatt ttttctaatg tggatagata tgaattattt ttctccttaa ggatcatccg
    16501 ttatttgggt cattttctgc taaaatctgc gccttgcgct tctttagtgc actgtctatg
    16561 gctaaccttt tgaataaatt cattatgacg agaatactcg ctgcgataac ccttttgctg
    16621 agtatcgtat tgaccatttt ggtcactatt ttttgttccg tcccgatcat cattgccggg
    16681 attgtaaaac tattgctacc tgtgccagtc atttggcgaa aggtttcgcg tttttgtgat
    16741 tttatgatgt attgctggtg tgaaggtctg gcggtattac tgcatctgaa cccacacctg
    16801 caatgggaag ttcacgggct ggaagggtta agtaagaaga actggtatct gcttatttgt
    16861 aaccaccgta gttgggcaga tattgtcgta ctgtgcgtgc tgtttcgtaa gcacattccg
    16921 atgaataaat atttcctcaa gcaacagctg gcctgggtgc catttcttgg cctggcgtgc
    16981 tgggcgctgg atatgccgtt tatgaagcgt tattcccgcg cttatttatt acgtcatccg
    17041 gagcgacgtg gtcaagatgt tgaaaccact cggcgttctt gtgaaaagtt tcgcctgcat
    17101 cccaccacca ttgtcaattt cgttgaaggc tcccgtttca cgcaagaaaa acatcagcaa
    17161 acccactcca cttttcaaaa cttgttgcca ccaaaggcgg caggcattgc gatggcgcta
    17221 aatgtactgg gtaaacaatt cgataaactg cttaatgtta cgctgtgtta tccggacaat
    17281 aaccgtcagc cgttcttcga tatgttaagc ggtaaactga cgcggattgt cgtacatgtg
    17341 gatttacagc ctattgccga tgagttacat ggcgactaca tcaatgacaa aagctttaag
    17401 cgccattttc agcaatggct gaactcattg tggcaggaaa aagaccggct tctgacgtct
    17461 ctcatgtcat cacagcgtca gaataagtaa gttttactcg cctctgaatg agcagaggcg
    17521 agtgagtatt ttaatgaaca aaacgtccgg cacgagacat aaattcttct tcgctcatct
    17581 cctgtccgtt aaaaacaact ttacccggtg tataacgcag ctgcagcgag gcggtattgt
    17641 tgtcaatcgt gattaactga aacatctgac ctaatgtcat catcccgctg atttgtttat
    17701 cagcctgctt ttgcgctttt tccgcatcca ttccttcaga taaattaagc tgttttgcgg
    17761 tttctgtgac gacattaagc ggtaacttca catcaaaatt gagcgatttg atatctttgt
    17821 ttgtggatga tgaagatttg gctgggtcgg caatactgat atccagattg gcatttagtt
    17881 cgccgagtgc gttcttccat cttaccggtt gtttaatggt cggctcactt ttttgtaata
    17941 acggtaaata ctctttgaac aatgcagcat tcacttcttg cagagcaact tcatcgtttg
    18001 ctaactcagg gtgtgcagca agctgcttct gcatcgcaat gttatactgg atgataaatt
    18061 ggcgcactgc tgagggatca atagattcag caatcaatga ccactcacca ctaccgagtt
    18121 gctgattccc ttttgttaac ttatcaagtt cgtaggccag attgacattg atataatctt
    18181 tgtctttaac gcgatccagc ctggtttttg caacgaaagc atcgatttgg gcaacgtctt
    18241 ccccgtgatt gataatgtta atctgattaa atttcgactc gctttcacca accggaaatt
    18301 tcttttcttc taatcgcgcc agagaatcaa tggtaaaact ttttgccgtt actttgttca
    18361 ttggtgataa ttgcagaaca atatcttcga ctttgccttc aatatccagg ttagacaatg
    18421 aactaccatc accattaaaa gtaaactggc cctcaccaaa agaaaatttt ccatactctg
    18481 caggggcaac atctaatgtc gtagagaatg agccaccaaa cgcgaatcgc aatgtcgctt
    18541 ccacgggcga tttatttttc gcggcgataa atagcggttg tgtcagttca ttattcacta
    18601 agttcacttt tgccgctgcc agtgctggga tgacattacc atgcattaac attgtgatgg
    18661 gcaacggacc gtgctcaaca tccacatcaa atacaacttt ctggcctggc ttgatattga
    18721 gatcgggtgc accgttatcg aatgtcattt gcatttgaaa acgggaatta aaaaagcctt
    18781 tgtcaaagtt tttataactt aacgtcatat cgtaggcatg ttcacctttc tttttcgcat
    18841 cgttaaaatc tttaataaac ttttcgatac caggctgaat ttgcgtacct gtgtaccatg
    18901 tgccaccacc ccagattacg gctaacgcaa caataacacc tgtagctgac ttacgtatca
    18961 tataaatatt ccctgataaa acagcaaaac tttgaatatc cacttatgct gaaataatgt
    19021 ccagcggcag gatgcattat cagaatgtat ttatatttat taaataataa aaaaagcccg
    19081 tgaatattca cgggctttat gtaatttaca ttgaattatt tttcctcgga cagatatttc
    19141 accgtatcag catactgctg aacaaaaaca tccatattgc tggtatccat accctgcgga
    19201 ttcagctgat atttaccgtt aacaaacatc gccggaacac cacgcaattg cacatcagct
    19261 gcggcttttt cctgctgagc gaccagagat ttcaccacga agctgttcca cgccgcgtcg
    19321 tactcttcac ctttaatacc tgcattgata aatacatcgc ggatatcaga tgctgaacga
    19381 atggtctggg ttttctgtac gccttcaaac agcggaacag tcactttgtc ttccacgccc
    19441 agcgccatcg ccacagccca tgcctgagtc agatctttgc ccaggtcacc acccatgaag
    19501 ttgacgtggt atttagtcat cttcacgcct tccggcagtt ttttcttcac attatcagaa
    19561 atatgcagaa cttcttcaaa ctgatagcag tgcgggcaga agaaagagaa aaactccagc
    19621 acttgcggcg cgccagctac cggtttttcc agggtagtgt actgtttacc atcttcatac
    19681 tgcgccgccg atgcgctaaa cgctaaaact aaaccagcca gcgccagcca aatctttttc
    19741 atgatctact ctctccgatt aatacatagg tgttaattgc aaagggggtt cttgtagaac
    19801 ttttgcctgt tctataaaag tcgccgtctg tcgtagccag taatcttccc cggttaacca
    19861 cgggaaattt ttcgggaacg cgggatcagc ccaatgccgc attagccagg caagataata
    19921 aaccaaacgc atggcgcgta aaggttcaat cagtccgatt tcagcggtgt cgaactcgct
    19981 aaattcttca taagcttcaa taatagtttc cagttgcatc cgctgctggg ctttatcgcc
    20041 attgagcaac atccacaaat cctgaatagc tggaccatta cgtgcatcat ccagatcaac
    20101 aaacattgga ccatcgcgcc agagaatatt cccggcgtgg cagtctccat gtagccgcag
    20161 aacggtgaaa tcttcccgcc agtgtgctgt aacggcggcg atcagctcat ctgtcgcttt
    20221 cagaaatgcc gctttcaacc cggaaggtat cagtgtagcg tcctcaaaca gcttgcgagg
    20281 ctcaatgaga tattcattca gaccgatggt cgggcgatga ataaaaagct gtttgcgccc
    20341 cgtctggtgc atacgcccta aataacgccc aaccgcttcc atctgatcga tattatcagc
    20401 ttcgaactgg cgaccaccga cgcttggaaa aacagcgaaa taaaatccct gatgattcaa
    20461 taaagtctga ccgttaaagg ccacaggtgc tgcgaccgga acttcatcat ttaccagctg
    20521 caacgcaaat tgatgttctt cgaggatttg atcggctgtc caacgttcag ggcgataaaa
    20581 tttgacgaca aaacgtcgac gctcttcgtc ctgaaattga tagacacggt tttcatagct
    20641 gttaagcggg gtaagaccgg aatccacccg gatcccttgc ttaaacagag cgtccatgat
    20701 ggtatccggg tgtagtgtct ggaaagtaaa agcgctgttg ttcatcccat catccggaaa
    20761 acattacgaa tgatgaagga tatcatttca cgatgatttt ggtggcgccg cttacaagct
    20821 tttactcttt aattacgccg cgagcgcgta acagcgccgt tttgaagtct tcttcataat
    20881 ctttctgcaa acccgggatc accgcatctt tcgcggaatc acgcattttc aggtgataga
    20941 tcagaatgtc atccgtcaaa tccgccagtt cgccgtcaaa acctgactct ttcgccagtt
    21001 tctgcaaaaa ttgcaacaag ttaaagtctg gctctttttg ccaggctggc tggaggagtt
    21061 caataacttc attcagacgt ttacatttca tagtaatgct ccttactctt gagacagaca
    21121 cgttagcagg gtcaatccca caataaaaga ggcgatatcg gtgaatctaa tgacgacgat
    21181 aacaggcgtt gtgctggcag gcggtaaagc cagacgaatg ggcggcgtag ataaaggatt
    21241 gctggactta aatggcaaac cgctgtggca acatgtcgct gacgcgctta agacgcagct
    21301 ctctcacgtc gtggttaatg ctaatcgtca tcaggaaatc tatcaggcag gcggtctgaa
    21361 agtgattgaa gattcactgg cggattaccc aggccctctg gcaggaatgc tttcagtaat
    21421 gcagcaggaa gctggtgagt ggtttttgtt ttgtccgtgc gatacgcctt acattcccca
    21481 tgatttagcc gcccggctta atcatcagcg caaagatgcg cctgtcgtgt gggtccatga
    21541 cggtgaacgc gatcacccga ctattgctct ggtaaaccgc gctattgagc ctttattact
    21601 ggaatatctg caagcaggag aacgccgggt aatggcattt atgcgtctgg ctggcggtca
    21661 tgcggttgat ttcagcgatc ataaagatgc atttgttaac gtgaatacgc cagaggagct
    21721 tgcccgatgg caggaaaaac gatgataccg ttactcgcct ttgccgcgtg gagtggtacc
    21781 ggaaaaacta cgcttttgaa aaaactgatc ccggcattat gcgccagagg gatccgtccg
    21841 gggctgatta agcatacgca ccatgatatg gatgttgata agccaggcaa agatagctat
    21901 gagctgcgca aggctggcgc ggtacaaacc atcgttgcca gccagcaacg atgggccttg
    21961 atgacggaaa caccagacga agaagagctg gatctacatt ttctcgcaag tcgaatggat
    22021 acctcaaagc tggatttgat tctggtcgaa gggtttaagc atgaagagat cgcaaagatt
    22081 gtgctgtttc gcgatggagc cggacatcga cctgaagaat tagtgataga caggcatgtt
    22141 attgctgtag ccagtgatgt gccgcttaat cttgatgtcg cattactgga tattaatgat
    22201 gttgaggggc tggctgattt tgtggtggag tggatgcaaa agcagaacgg ataagcaaga
    22261 gtaggctgga tcagacgc
//
LOCUS       DAZPCS010000050        22168 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZPCS010000050 DAZPCS010000000
VERSION     DAZPCS010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22168
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.050"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(481..1572)
                     /gene="ychF"
                     /locus_tag="ACHUQ7_001919"
     CDS             complement(481..1572)
                     /gene="ychF"
                     /locus_tag="ACHUQ7_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFR1135351.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1689..2273)
                     /gene="pth"
                     /locus_tag="ACHUQ7_001920"
     CDS             complement(1689..2273)
                     /gene="pth"
                     /locus_tag="ACHUQ7_001920"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFR1135352.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGSEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2551..2829
                     /gene="ychH"
                     /locus_tag="ACHUQ7_001921"
     CDS             2551..2829
                     /gene="ychH"
                     /locus_tag="ACHUQ7_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFR1135353.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(2884..4563)
                     /gene="dauA"
                     /locus_tag="ACHUQ7_001922"
     CDS             complement(2884..4563)
                     /gene="dauA"
                     /locus_tag="ACHUQ7_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFR1135354.1"
                     /translation="MNKIFSSHVMPFRALIDVCWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(4688..5635)
                     /gene="prs"
                     /locus_tag="ACHUQ7_001923"
     CDS             complement(4688..5635)
                     /gene="prs"
                     /locus_tag="ACHUQ7_001923"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFR1135355.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5786..6637)
                     /gene="ispE"
                     /locus_tag="ACHUQ7_001924"
     CDS             complement(5786..6637)
                     /gene="ispE"
                     /locus_tag="ACHUQ7_001924"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFR1135356.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(6637..7260)
                     /gene="lolB"
                     /locus_tag="ACHUQ7_001925"
     CDS             complement(6637..7260)
                     /gene="lolB"
                     /locus_tag="ACHUQ7_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFR1135357.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7474..8730
                     /gene="hemA"
                     /locus_tag="ACHUQ7_001926"
     CDS             7474..8730
                     /gene="hemA"
                     /locus_tag="ACHUQ7_001926"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFR1135358.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFVDSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            8772..9854
                     /gene="prfA"
                     /locus_tag="ACHUQ7_001927"
     CDS             8772..9854
                     /gene="prfA"
                     /locus_tag="ACHUQ7_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFR1135359.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9854..10687
                     /gene="prmC"
                     /locus_tag="ACHUQ7_001928"
     CDS             9854..10687
                     /gene="prmC"
                     /locus_tag="ACHUQ7_001928"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFR1135360.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10684..11076
                     /gene="sirB2"
                     /locus_tag="ACHUQ7_001929"
     CDS             10684..11076
                     /gene="sirB2"
                     /locus_tag="ACHUQ7_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFR1135361.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            11080..11889
                     /gene="sirB1"
                     /locus_tag="ACHUQ7_001930"
     CDS             11080..11889
                     /gene="sirB1"
                     /locus_tag="ACHUQ7_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFR1135362.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDGWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVERCPEDPISEMIRAQINNIAHKHIVLH"
     gene            11925..12779
                     /gene="kdsA"
                     /locus_tag="ACHUQ7_001931"
     CDS             11925..12779
                     /gene="kdsA"
                     /locus_tag="ACHUQ7_001931"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFR1135363.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(12928..13035)
                     /locus_tag="ACHUQ7_001932"
     CDS             complement(12928..13035)
                     /locus_tag="ACHUQ7_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR1135364.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIIAAAIVSWWRNRK"
     gene            complement(13440..14540)
                     /gene="chaA"
                     /locus_tag="ACHUQ7_001933"
     CDS             complement(13440..14540)
                     /gene="chaA"
                     /locus_tag="ACHUQ7_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFR1135365.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLVLVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQAKTHQSLFVYEHEDDSDDDDPHHGKTSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            14810..15040
                     /gene="chaB"
                     /locus_tag="ACHUQ7_001934"
     CDS             14810..15040
                     /gene="chaB"
                     /locus_tag="ACHUQ7_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFR1135366.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            15198..15893
                     /gene="chaC"
                     /locus_tag="ACHUQ7_001935"
     CDS             15198..15893
                     /gene="chaC"
                     /locus_tag="ACHUQ7_001935"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFR1135367.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(15937..16290)
                     /gene="ychN"
                     /locus_tag="ACHUQ7_001936"
     CDS             complement(15937..16290)
                     /gene="ychN"
                     /locus_tag="ACHUQ7_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFR1135368.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            16475..17869
                     /gene="ychO"
                     /locus_tag="ACHUQ7_001937"
                     /gene_synonym="iatA"
     CDS             16475..17869
                     /gene="ychO"
                     /locus_tag="ACHUQ7_001937"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFR1135369.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(17870..18520)
                     /gene="narL"
                     /locus_tag="ACHUQ7_001938"
     CDS             complement(17870..18520)
                     /gene="narL"
                     /locus_tag="ACHUQ7_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFR1135370.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18513..20309)
                     /gene="narX"
                     /locus_tag="ACHUQ7_001939"
     CDS             complement(18513..20309)
                     /gene="narX"
                     /locus_tag="ACHUQ7_001939"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFR1135371.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            20648..22039
                     /gene="narK"
                     /locus_tag="ACHUQ7_001940"
     CDS             20648..22039
                     /gene="narK"
                     /locus_tag="ACHUQ7_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFR1135372.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATGTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
BASE COUNT         5482 a         5504 c         5719 g         5463 t
ORIGIN      
        1 acaatatgcg ccatttttgc attactaata agaaaaagca aaaaacatcg atattcattt
       61 ttgttcaatg gattttctgg cctatcgtta ttattgtgct actggttatc attttcatct
      121 tgccaggatc gcacgtaatc acgttattcg gggactttat ctgcgggata ttacccttca
      181 ggtaagtcct tttctcgcca gcatattgga gcgagatcaa tattgatcag ctgtgaagag
      241 ggctgatgtg tagtggaaca aaaactgacg ttatatcact gctaatgaga tatcagtcat
      301 ctgaacactt atgtctttaa ttattctcgt ggttcactgt aggcaataag cacaaaagtg
      361 taggatgtta caagaatgat taggactcgg tgaaatgaaa aatccacgca attgcgtgga
      421 ttttatatac ttttgcgctc ttcatgagat ttagcgaaac ctcatgagac aataaattaa
      481 ttagacgttg aaaaggaagt tcatcacatc gccatctttc acgatgtaat ctttaccttc
      541 tgcacgcatt ttgcctgctt ctttcgcgcc ttgttcacct ttgtaagtga tgaaatcttc
      601 aaacgagatg gtttgtgcac ggataaagcc tttttcaaaa tcagtatgga ttttgcccgc
      661 tgcctgcggc gcggttgctc caaccggaat ggtccatgca cgcacttctt tcaccccagc
      721 ggtgaagtaa gtttgcaggt tcagcagctt ataaccggca cggatcacac ggttcaggcc
      781 cggttcttcc agcccaagct cctgcataaa ctcgtcacgt tcttcgtcgt ccagttcggc
      841 aatgtctgct tcaacagcag cacaaaccgg aaccacaaca gaaccttctt tcgccgcgat
      901 ttcacgcacc tggtcaagat atgggttgtt ttcaaaacca tcttcgttga cgttggctat
      961 gtacattgtt ggtttcagcg tcaggaagct caggtaacga atagccgctt tctcttcagc
     1021 gcttaaatcc agcgcgcgca gcatacctgc gttttccagc tggggcaggc atttttccag
     1081 gaccgccagc tcagctttcg cgtctttatc gccacctttg gctttcttct gtacgcgatg
     1141 aatcgcacgt tcgcaggtgt cgaggtctgc cagcgccagt tcggtgttga taacttcaat
     1201 atcgtcagcc gggttaactt tgccggaaac gtgaatgatg ttgtcatttt caaagcagcg
     1261 aacaacgtga ccgatcgctt cggtttcacg gatgttggtc aggaactggt tacccagacc
     1321 ttcgcctttc gatgcgcctt ttaccagacc ggcgatatcg acaaattcca tggtcgtggg
     1381 aagcgtacgt tgcggtttta cgatttcagc cagttgatcc aggcgagggt caggcattgg
     1441 tacgacgcct gtgttcggct caatggtgca gaatggaaag ttggccgctt caataccggc
     1501 tttggtcagc gcgttgaaca gggtagattt cccgacgttg ggcaaaccga cgataccgca
     1561 tttgaatccc atgatttaaa tcaccttaat atcttaataa tcaacctgtt attgctaaca
     1621 gattgcagaa atggaaataa ctttgcctat tatacacggc actcggcaaa aatgccgcag
     1681 acaacgactt attgcgcttt aaaggcgtgc aatcggttcg ttgctttggt caagccatct
     1741 gtaaaccaca tttcagtaca acgcgccgct tcgtcgatgg cttcatcaat taacttctgt
     1801 tcactaacag gcggtttgcc taacacaaaa ccgacaactt tatttttatc gcccggatga
     1861 ccgattccga tgcgtaaacg gtgaaagtta gggttattac ccaatttact gatgatgtct
     1921 ttcagtccat tgtgaccacc atggccaccg cccaacttaa atttggcgac gccaggaggc
     1981 agatccagtt cgtcgtgggc caccagaatt tcgtccggat taatgcggaa aaaactggcc
     2041 atcgccgcaa cggctttgcc gctgagattc ataaatgtag tcgggactaa caggcggaca
     2101 tcttcgcttc caagggtgac tcgcgaagta taaccaaaga atttagcctc ttcgcgcagc
     2161 ggagcgcgca aacgctctgc cagtaagtca acgaaccagg caccagcatt atgtcgcgtt
     2221 gcggcgtatt cagcaccggg gttcgccagg ccgacaatca atttaatcgt cacgtttttt
     2281 tgtcctgagt gtgtacataa ctggcgcgta gtttactggt tgcggccccg cttgacaaaa
     2341 aactgcgtat caaatgcaga taacgtaata attgcctgag tggactatta gaaagtcaag
     2401 gtgttcaggc gtttatttgt aaagttttgt tgaaataagg gttgtaattg tgattacgcc
     2461 cgcacataac ccacagggtg ttgtctatac tttacacata aggaagaggg gtattccctg
     2521 ttacaaccca gaaagttccg gaggtgacat atgaaacgca aaaacgcttc gatactcggt
     2581 aacgtgctca tggggttggg tctggtggta atggtggtcg gcgtggggta ttcaatcctc
     2641 aaccagttac cacagtttaa tatgccccag tatttcgcac atggtgcagt gctaagtatt
     2701 ttcgtcggtg ccattctctg gctggcgggt gcccgtgttg gcgggcatga acaggtgtgc
     2761 gaccgttact ggtgggttcg tcactatgac aaacgttgcc gccgtagcga taatcgccgt
     2821 catagctaac aaataatgca gtttgctgac cagtcagttt gcgctgactg gtcaatctcg
     2881 tacttataaa tccgccatcg ccgcgcgacg attcgggaag aacgcgaggc gtcccgggat
     2941 cggttgaatg cctgcgcgag ccatagttcg cagtggctgg aattccacgt tgcacacgcg
     3001 cagttcacat ccttcgggca gacgcttcac aaaacgctgg aacgcatcaa gaccaccagc
     3061 atcaagtacc ggaacggcat cccacttcag aatcacaatc cgtttgcctt caagacgtga
     3121 ctccaggtcc gtgaataagc cttcagcagc agcaaaaaac agcgggccaa taacgcgcag
     3181 aaccaggaca tcgtctggaa catctacgac taccggtgcc aggcgagtca tacgtgcgat
     3241 acgacgcata aacagcagcg atgccagcac gatccccacg ctgatggcaa taaccatatc
     3301 aaacagcacg gtcagtgaca tacacagcag catgacgatg atgtcatctt tcggcgcatg
     3361 acgcagcaag tcgaccactt tgtgcgcttc actcatgttc cacgccacca tcaacagcag
     3421 ggctgccatg gcggaaagcg gcagccagga gagcagtggt gccagtacca gcagggcgag
     3481 aataaccaga atagagtgga tcaccgccga gataggggaa gttgccccgg cacggacgtt
     3541 agcggcagaa cgcgcgatgg cagcagtagc ggtaatacca ccaaagaatg gagcgatgat
     3601 attccccagc ccctgaccga ccagttcact attcgccttg tgcttagtcc cggtcatacc
     3661 atccagtacc acagcgcaga gcagagattc gatcgccccg agcattgcca ttgagaatgc
     3721 cgcaggcagc agggtgcgaa tagaatccca ggttagcgtg aattctgaat taggcagatc
     3781 ccacggcagc accagttgcg gcagcagttg cggaataccg ttaccctgag aaccatcggc
     3841 caggacgtag tggaattgcg aaccgatggt agcaacatgt ccgccgagca ggttaacaat
     3901 ccccatcacc gcgcaaccag ccagcaaggc cggaaggtga ccgggtaaac gaatgcccag
     3961 acgcggccag aaaaccagaa tgccaagcgt cacaatgcca atggcagcat cacccacatt
     4021 aatggtcggc agcgccataa ataatgcgcc gactttttgt agataatgtt ccgggacatg
     4081 ggccatttgc agaccgagaa aatctttaat ctgcatggta ccgatggtga tcccgatacc
     4141 cgaggtgaaa cctaaggtga cggaaaccgg aatatactca atcagacgac caaagcgtgc
     4201 cagccccatc agaatcaaaa agattcccga caacaaggtc gcaaccagca gtcctgccag
     4261 tccaaactgt tgcgaaacgg gatagagaat taccacaaat gccgcagtcg gaccggaaac
     4321 gctaaagcgt gacccacccg ttagagcaat gacaatcccc gcaacagctg cggtatataa
     4381 accgtactgg ggggccacac cactaccaat agccaacgcc atcgccagcg ggatagcaat
     4441 aatcccgacg gttatcccgg caatcaggtc acgggtaaac cgtgcggcag tatatttttc
     4501 tttccagcaa acgtcgatca gagcgcggaa aggcatcaca tgtgaggaaa atattttgtt
     4561 cacaataatg tttcatccgt gagcgcatca tctgtcaact aaatggcagg tgaaggaggc
     4621 ataggtcata caaatggata ttacagacaa aaaaacccgc cgcagcgggt ctttgagccg
     4681 ggttcgatta gtgttcgaac atggcagaga tcgattcttc gttgctgata cgacgaatcg
     4741 cttcggccag catacctgac agggtcagag tacgcacgtt cggcagtgat ttgatttcat
     4801 cgctcagcgg aatggtatcg cagacaacga cttcatcaat tacagagtta cgcaggttgt
     4861 tcgccgcgtt gccagagaag atcgggtgag tcgcgtacgc aaatacacgt ttagcaccac
     4921 gttctttcag cgcttcagca gctttacaca gcgtaccgcc agtgtcgatc atatcatcga
     4981 ccagtacgca gtcacgacct gcaacgtcac cgatgatatg catcacctgg gaaacgttcg
     5041 cacgcggacg acgtttgtcg atgattgcca tatcggtatc gttcagcagc ttagcgatag
     5101 cgcgggcacg cacaacgccg ccgatgtccg gagaaaccac aattgggtta tccagattca
     5161 gctgcagcat gtcttccagc aggatcgggc taccaaatac gttatcaacc ggaacgtcga
     5221 agaaaccctg aatctgttca gcgtgcagat ccactgtcag cacacggtca acaccgacgc
     5281 tggagaggaa gtctgcaacc actttcgcag tgattggtac acgagcggaa cggacgcgac
     5341 ggtcctggcg cgcatagcca aagtagggga taacagcggt gatacgacct gcggaagcac
     5401 gacgcagggc atcaaccata acgactaatt ccatcaggtt gtcgttagta ggggcacaag
     5461 tggactggat gatgaaaata tcaccaccgc gtacattttc gttaatttgt acgctgactt
     5521 cgccgtcgct aaagcgacct acagcggcgt cgccgagtga agtgtacagg cggttggcaa
     5581 tacgttgtgc tagtaccggg gtggcgttac cagcaaaaag cttcatatca ggcacgagaa
     5641 gaacctcagg catgcgtcca ttggtggaaa gaatctgccg aaaactgtgc gggccaggca
     5701 tgatcctttc caggcggtgt attaaagagc gcgatgcaac gtctggaaca aggtgacgtt
     5761 gtcaccgaaa ctcagcttgc ccggcttaaa gcatggctct gtgcaatggg gaaagattaa
     5821 cgcctttcgc cacaaagcca ttgagccatt ccggggcttg ctctagcacc tggcgggctt
     5881 cagactctgt atcaaattca gcaaagacac aggcccctgt cccagtcagg cgtgacgggg
     5941 cgtattctaa cagccaggaa agcaccgcat caacctcgcg aaaacgtttt cttgcgataa
     6001 cctcgcaatc attgctgaat tcacatttta gcaacgtttc tattgacctt tttggcgtat
     6061 tgcgcgggag ttcaggatct ttaaaaatca ccggagtcgg aatacttaca ccagggtgcg
     6121 ccaccagata ccacttctct ggcggatcca ccggcgttag tatttcacca acgccttcgg
     6181 caaacgcggc atgcccccga acaaagacag gaacatctgc gcccagcgtc agccccattt
     6241 ccgccagctc atccatgctt agcccgcatt gccagagatg atttaatgcc accaggaccg
     6301 tcgcggcatt ggatgaacca ccgccgagac cgccgcccat cggcaaacgc ttgtcaatgc
     6361 tgatattcgc accgcttccc gtcggaagac gcccgctgtc tgccgcagtt ttcatcaaca
     6421 accgcgctgc gcgaacgatc aggttatctt catgttccac gccttcaacg ggcgttaaca
     6481 gacgaatatc cccatcgtca cgaagctcaa tgctgatggt gtcgccgtaa tcaagaaact
     6541 gaaacagcgt ttgcagcgtg tggtaaccat ccgcacgctg accggtaatg tataaaaaca
     6601 gattaagttt tgccggagag ggccactgtg tccgcattat ttcactatcc agttatccat
     6661 ttttaacttg atgcgttgac caccgtcggt gagttccata ttggctggca tcgcaggttg
     6721 cgttttggtg tcataaccac cataaacaac cttccagttt ttgccattct ggctgtaggt
     6781 aatttcgctc aggcggtact ggtcgtccag tttgtagtcg gttgcatcac ccggtaaacc
     6841 taaaatccac tggcgcaagc tgttgagcgg aattggcatt ccggtcaatt tgccaatcat
     6901 ctcttcggcg tcatcggcgg tataacgctg acctttattg tcgactaact gcacgttacc
     6961 cggttgagca ttcagctcca gttccgtgct gcccaatggg ttagtgagca gcagacggta
     7021 gcgatcctgg ccggtttgct gccagaaaaa gcgggcgtac actttttgtt ggtcagaaat
     7081 ataagcgaac gcgccgcgag tctgatactg attaagattg cgcacgtctt gctgatgctg
     7141 acgccattgt ggcgaatccg ggcttttgcc aggacctttg ggcgtggtaa cggaacaggc
     7201 agtgagcaca agagcagcca gcggtagcag gcggataaga cgaaaatcgg gcaggggcat
     7261 agtgatgaca agtccttgag atacgttgca gttataaccc ttaatgctag cgttaccgtc
     7321 cgctatcgtc tatgttcaag ttgtcttaat tgccagaatc taacggcttt cggcaattac
     7381 tccaaaaggg ggcgctctct tttattgatc ttacgcatcc tgtatgatgc aagcagacta
     7441 accctatcaa cgttggtatt atttcccgca gacatgaccc ttttagcact cggtatcaac
     7501 cataaaacgg cacctgtatc gctgcgagaa cgtgtatcgt tttcgccgga taagctcgat
     7561 caggcgcttg acagcctgct tgcgcagccg atggtgcagg gcggcgtggt gctgtcgacg
     7621 tgcaaccgca cggaacttta tcttagcgtt gaagagcagg ataacctgca agaggcgtta
     7681 atccgctggc tttgcgatta tcacaatctt aatgaagaag atctgcgtaa aagcctctac
     7741 tggcatcagg ataacgacgc ggttagccat ttaatgcgtg ttgccagcgg cctggattca
     7801 ctggttcttg gggagccgca gatcctcggt caggttaaaa aagcgtttgt cgattcgcaa
     7861 aaaggtcata tgaaggccag cgaactggaa cgcatgttcc agaaatcttt ctctgtagcg
     7921 aaacgcgttc gcactgaaac agatatcggt gccagtgctg tgtctgtcgc ttttgcggct
     7981 tgtacgctgg cgcggcagat ctttgaatcg ctctctacgg tcacagtgtt gctggtaggc
     8041 gcgggcgaaa ccatcgagct ggtagcgcgt catctgcgcg aacataaagt acagaagatg
     8101 attatcgcca accgcactcg cgaacgtgcc caaattctgg cagatgaagt tggcgcggaa
     8161 gtgattgccc tgagtgagat cgacgaacgt ctgcgcgaag ccgatatcat catcagttcc
     8221 accgccagcc cgttaccgat tatcgggaaa ggcatggtgg agcgcgcatt aaaaagtcgt
     8281 cgtaaccaac caatgctgtt ggtggatatt gccgttccgc gcgatgttga gccggaagtt
     8341 ggcaaactgg cgaatgctta tctttatagt gtggacgatc tgcaaagcat catttcgcac
     8401 aacctggcgc agcgtaaagc cgcagcggtt gaggcggaaa ctattgtcgc tcaggaaacc
     8461 agcgaattta tggcgtggct gcgagcacaa agcgccagcg aaaccattcg cgagtatcgc
     8521 agccaggcag agcacgttcg cgatgagtta accgccaaag cgttagcggc ccttgagcag
     8581 ggcggcgacg cgcaagccat tatgcaggat ctggcatgga aactgactaa ccgcttgatc
     8641 catgcgccaa cgaaatcact tcaacaggcc gcccgtgacg gggataacga acgcctgaat
     8701 attctgcgcg acagcctcgg gctggagtag cagtacatca ttttcttttt ttacagggtg
     8761 catttacgcc tatgaagcct tctatcgttg ccaaactgga agccctgcat gaacgccatg
     8821 aagaagttca ggcgttgctg ggtgacgcgc aaactatcgc cgaccaggaa cgttttcgcg
     8881 cattatcacg cgaatatgcg cagttaagtg atgtttcgcg ctgttttacc gactggcaac
     8941 aggttcagga agatatcgaa accgcacaga tgatgctcga cgatcctgaa atgcgtgaga
     9001 tggcgcagga tgaactgcgc gaagctaaag aaaaaagcga gcaactggaa cagcaattac
     9061 aggttctatt actaccaaaa gatcctgatg acgaacgtaa cgccttcctc gaagtccgtg
     9121 ccggaaccgg cggcgacgaa gcagcgctgt ttgcaggcga tctgttccgt atgtacagcc
     9181 gttacgccga agcccgccgc tggcgggtag aaatcatgag cgccagcgag ggtgaacatg
     9241 gtggttataa agagatcatc gccaaaatta gcggtgatgg tgtgtatggt cgtctgaaat
     9301 ttgaatccgg cggtcatcgc gtgcaacgtg ttcctgctac ggaatcgcag ggtcgtattc
     9361 atacttctgc ttgtaccgtt gcggtaatgc cagaactgcc tgacgcagaa ctgccggaca
     9421 tcaacccagc agatttacgc attgatactt tccgctcgtc aggggcgggt ggtcagcacg
     9481 ttaacaccac cgattcggca attcgtatta ctcacttgcc gaccgggatt gttgttgaat
     9541 gtcaggacga acgttcacaa cataaaaaca aagcgaaggc gctttcagtg ctcggtgctc
     9601 gcatccacgc tgctgaaatg gcaaaacgcc aacaggccga agcgtctacc cgtcgtaacc
     9661 tgctggggag tggcgatcgc agcgaccgta accgtactta caatttcccg caggggcgcg
     9721 ttaccgatca ccgcatcaac ctgacgctct accgcctgga tgaagtgatg gaaggtaagc
     9781 tggatatgct gattgaaccg attatccagg aacatcaggc cgaccaactg gcggcgttgt
     9841 ccgagcagga ataatggaat atcaacactg gttacgtgaa gcaataagcc aacttcaggc
     9901 gagcgaaagc ccgcggcgtg atgctgaaat cctgctggag catgttaccg gcagagggcg
     9961 tacttttatt ctcgcctttg gtgaaacgca gctgactgac gaacaatgtc agcaacttga
    10021 tgcgctactg acacgtcgtc gcgatggtga acccattgct catttaaccg gggtgcgaga
    10081 attctggtcg ttgccgttat ttgtttcgcc agcgacctta attccgcgcc cggatacgga
    10141 gtgtctggtg gagcaggcac tggcgcggtt gcctgaacaa ccttgccgta ttctcgatct
    10201 cgggacgggt accggggcga ttgcgcttgc gctggctagc gagcgcccgg actgcgaaat
    10261 tatcgctgta gatcgtatgc ctgatgctgt ctccctggca caacgtaatg cccagcatct
    10321 ggcgatcaaa aatatccaca ttctgcaaag cgactggttt agcgcgctag ccgggcagca
    10381 gtttgcgatg attgtcagca atccgccgta tattgacgag caggacccac atcttcaaca
    10441 aggcgatgtc cgctttgagc cgctcactgc gctggttgcg gcagacagtg gaatggcaga
    10501 catcgtgcat atcatcgaac agtcgcgtaa cgcgctggta tccggcggct ttctgcttct
    10561 ggaacatggc tggcagcagg gcgaagcggt gcgacaagca tttatcctcg cggggtatca
    10621 tgacgtcgaa acctgccgtg actatggtga taacgagcgc gtaacgctcg gccgctatta
    10681 tcaatgacaa gtttttctac actgcttagt gttcatctta ttagtatcgc gctttctgtt
    10741 gggctattaa ccttacgttt ctggctacgt tatcagaagc atccacaggc atttgctcgc
    10801 tggacgcgca ttgtgccgcc ggttgtcgat acggtgttat tgttaagcgg cattgcgttg
    10861 atggctaaag cgcacatcct gccattttcc gggcaggcac agtggctgac tgaaaagctg
    10921 tttggagtta tcatttatat cgttttgggt tttattgcac tcgattatcg tcgtatgcac
    10981 agtcagcagg cgcgcattat tgccttcccg ctggcgttgg tggtgctgta catcatcatt
    11041 aaactcgcca ccacaaaagt accgttactg gggtaagtca tgagatcgtt agctgatttc
    11101 gaatttaata aagcgccatt gtgcgaaggc atgatcctgg cttgcgaagc aatccgccgc
    11161 gattttccct cgcaagatgt ttacgacgaa ctggagcgtc tcgttagtct ggcgaaggaa
    11221 gaaatcagcc agcttctgcc tttagaagag cagctggaaa aactgatcgc gctgttttac
    11281 ggcgactggg gatttaaagc ctcacgcggc gtttatcgtc tttccgatgc attatggctg
    11341 gaccaggtgt taaagaatcg acagggcagt gcggtatcat taggtgcggt tttattatgg
    11401 gtcgcgaatc gtctcgattt gccgctgctg ccggtgattt tccctacgca gctgatattg
    11461 cgcattgaat gtccggatgg cgaaatttgg ctgattaatc cttttaacgg tgaatcgtta
    11521 agcgaacata tgctggacgg atggttaaag ggaaatatca gcccgtcggc ggaactgttt
    11581 tatgaagacc ttgatgaagc tgataacatt gaggtaatcc gcaaattgct ggatacactc
    11641 aaagcctcgt tgatggaaga aaatcagatg gagctggcgt tacgcaccag cgaagcttta
    11701 ttacaattca accctgaaga tccctatgaa attcgcgatc gtgggttgat ttatgcgcaa
    11761 ctggattgcg aacacgttgc gttgaacgat ttaagttatt tcgttgaacg gtgtccggaa
    11821 gacccgatca gcgaaatgat ccgtgcgcaa ataaataaca tcgcgcataa acatattgtg
    11881 ctgcattaat taatcgacat tttactcaag attaaggcga tcctatgaaa caaaaagtgg
    11941 ttagcattgg cgacatcaac gtagcaaatg acctgccgtt cgtactgttt ggcggtatga
    12001 acgtgctgga atctcgcgat ctggcgatgc gcatttgcga gcactacgta actgtgaccc
    12061 agaaactggg tatcccttac gtgttcaaag cctcttttga caaagccaac cgctcctcca
    12121 tccactctta tcgtggaccg ggcctggaag aagggatgaa aatcttccag gagttgaagc
    12181 agacttttgg cgtgaaaatt atcaccgacg ttcacgaacc aagtcaggca cagcccgttg
    12241 ctgatgtcgt ggatgtgatt cagttgccgg cgtttcttgc tcgccagact gacctggttg
    12301 aagccatggc gaaaaccggt gcggtaatta acgtcaagaa accacagttt gtcagcccgg
    12361 gacagatggg taatatcgtt gataaattca aagaaggcgg caacgaaaaa gtgattcttt
    12421 gcgatcgtgg tgctaacttc ggctatgaca acctggttgt cgatatgctg ggcttcagca
    12481 ttatgaagaa agtgtctggt aactcgccgg tgattttcga cgtgacccac gcactgcaat
    12541 gccgcgatcc gtttggcgca gcttccggtg gtcgtcgtgc tcaggtggct gagctggcac
    12601 gagccggtat ggcggtaggt ctggcggggc tgtttattga agcgcatccg gatccggaac
    12661 atgcgaaatg tgatggtcca tccgcgctgc cgctggctaa actggaaccg ttcctcaagc
    12721 agatgaaagc gattgatgat ctggtgaaag gtttcgaaga actggatacc agcaagtaat
    12781 cttttttgct tgaaaaataa agtcttagcg ttctgcgtta agactttttt catgggtgcc
    12841 agatacaaaa aaggccgcag gctgttaccc ctgcggccgg tttcgggcgc atattgccat
    12901 cacggcagcc tgacgcccgt tttcacctta cttccggtta cgccaccagc tgacaatcgc
    12961 tgcggcaata atccccgcca ggatcggtgc tgccaggtcg tgccagaaag tcatggcaaa
    13021 ctgcgcgagc gtcatatagc cgccttgttg taatgacaac attttgcggc tattcttgaa
    13081 gtgtctggtt tcaagattag cccccgtttt gttgtcaggt tttacctctc aacgtgcggg
    13141 ggttttctct ttccagcaac caatgccacc agggataaag cccccgcaac attgcgcctc
    13201 accggataat tccggcttgg tgtggatact acgtctcaat tcatcttcac ttcatccctg
    13261 aaatgtttgc aataaagagt acattccggc ttttcaacag ctgttgcagt ggtttcgtgc
    13321 aggttgtggt acaggctcgc aattctgatt aacgagctgt ccaggtagta tgaagaaata
    13381 caataagcag gaatttatct tatctctccc gatttccgtc atcggaggag atataactgt
    13441 caggcaaata tcgtcatcaa ataggcggca aacagtgcca gatgcgctgc gccattgagc
    13501 acgttagtac gtccggtgga gaaggagata tggcacagca ctaaagaggc caccatcacc
    13561 accatttctg gcgcaccaag tgcaaactgc aattcgttac ccgtcataaa ggcaattagc
    13621 gtgacgacag gtacggtaag cgaaattgtt gctaacactg aaccaaagaa cagattcatc
    13681 gcgcgctgaa cctggttgtt caacactgct tttaacgcac ctaaaccttc cggcgacaga
    13741 atcaacagtg ccaccaggaa gccagtaaag gcaacagggg cattcatgct gtcgagcaat
    13801 gtctccagcg agctggcgtt cattttggtg actgcaataa cggcaatcag atggataatc
    13861 aaccagatag catgccacag gctgctatgg gcagacgttt taccgtggtg cgggtcgtcg
    13921 tcatcactgt catcttcgtg ctcgtagaca aacaaacttt gatgcgtttt ggcctggatc
    13981 agcaaaaata cgccatacat tgccgcagaa attaatgcta ccagtaacgc ctgaccggtt
    14041 gaaaaattcg ccgcaggcag agccattgga aataccagta cgattatcgc cagggggaac
    14101 agcgcaatta aatactgctt gataccaaac agattcatat attgggtggc aaacttacga
    14161 ccgcccaaca ataatgaaaa gccaaccagc ccaccggtaa caatcataat gattgaatag
    14221 agcgtatcac gcattagcgt tggcgcggcg tcgccggttg ccattaaagc tgaaatcaaa
    14281 ctgacttcaa gaataaccac tgaaaggcta agaataagcg aaccgtaagg ttctcccagg
    14341 cgatgggcta atacgtccgc atgacggaca acactaaagg cgctacttaa aataccaata
    14401 agcgcaagaa gattgatggc aatgaccact ggtagtgtct ggctacttcc ccacaggaac
    14461 agcactacca gcaccagaac cgggaaaata agcgaagtct ccttgtggcg ggtttttacc
    14521 gcctcttgag catttgacat tatggttatc cctttgcaga tgaatttatc gaaaatgtaa
    14581 aaaataggta ggaaaaataa cagaaagtgt ctggatatcg gtaacatttt acgaattttt
    14641 accccgctgt cgatttttta ctcattgggg cataaaaata agtattacgt ttagacaatg
    14701 tttgttttag cgtttcttaa gaacagtctg acatgaaaat tcttatgttt tggcaagagt
    14761 agatattgtt gaccacactt aatgttcaac tttgtaaaag gagtcaacga tgccgtataa
    14821 aacgaaaagc gatctgccgg aaagcgtaaa gcacgttcta ccgtctcatg cccaggatat
    14881 ctataaagaa gcgttcaaca gcgcatggga tcaatataaa gataaagaag atcggcgtga
    14941 tgacgccagt cgcgaagaaa ccgcgcataa agtggcctgg gctgctgtga agcatgaata
    15001 tgccaaaggg gatgatgata aatggcataa aaaatcgtaa aaccggtagc ttagttaaag
    15061 ctattcgtgc ggtgttgcct tgcaagtggt ccgtggattg catattgttc cgttagtggt
    15121 ttcaaaatga gcggtaaaaa tgtccggaag acaccaaaaa gttgtcgcag ggaagtatgc
    15181 agtggcggag gtgtaaggtg ataacgcgtg atttcttgat gaatgccgat tgtaaaacgg
    15241 catttggtgc cattgaagaa tcactcttat ggtcagcaga acaacgggcg gcttcgctgg
    15301 cggcgacgct ggcttgtcga cctgatgagg gaccggtgtg gatcttcggt tatggatcgt
    15361 tgatgtggaa tccggcactg gagtttaccg aatcatgcac cggtacactg gttggatggc
    15421 atcgcgcgtt ttgcctgcgc ctgaccgccg ggcgcgggac tgcgcaccag ccgggacgga
    15481 tgcttgcact gaaagagggc ggacgcacca caggcgtcgc ctatcgacta ccagaagaga
    15541 cgctggagca ggaactaacc ctgttgtgga agcgcgagat gattaccggc tgttatctgc
    15601 caacctggtg tcagcttgat cttgatgatg gacgcacagt aaacgccatt gtgtttatta
    15661 tggacccgcg acatccagaa tatgaatctg acactcgcgc tcaggtcatc gcgccgttga
    15721 ttgcggcggc gagcggtccg ctgggaacca atgcacaata cctgttttca ctggaacagg
    15781 agcttatcaa actgggaatg caggacgatg ggctgaatga tttgctggta tcggtaaaaa
    15841 aactactggc ggagaattat ccggatggtg tgttaaggcc gggattcgcc tgagtaaact
    15901 tccctcatag tggggcgtca gacgccccct taaacattaa aatgtgagca ctttatcggc
    15961 tgacagcgtc cattgcgcca gttccacaag agtaccgatt tccaccccat caatcagagg
    16021 aagtgtgcta atcccgcgcc cgtcggtaca ggttttgcac aatttcaccg gtacattctg
    16081 agcggtaagg atctccagca tttgctgaat gttgtagcct tcccctggtt tttgcccgcg
    16141 caacccggct gtgaccgcat cagacatcag gaacagacgc agatccagat tgctctcctg
    16201 ctctcgtaac gcaatggcca gccgcaagct gttaaacaag gattcgctcc cgtaaggtgc
    16261 gccattggca acgatcacta ttttttgcat tatttactcc tgtattcagg gaattagaca
    16321 ctcatcttct atcttactgc ttctgcagcg tctgaccaat cggtcacatt tttaaggatt
    16381 ttcctgaaag cgcgagaaaa tacgacaaaa gttgccagta atcgttattc tttaaggcta
    16441 tggtttttca ttattaccgg aagttaccga cgttttgagc cgtttcgttc ctcgcattat
    16501 cccgttttat ttactcttgc ttgtagcagg cggtacagct aacgcacaat ctaccttcga
    16561 gcaaaaagcg gcaaatccct ttgataataa caatgatggt ctgccggatt taggcatggc
    16621 tcccgaaaat catgatgggg aaaaacactt tgctgaaatt gtgaaagatt tcggcgaaac
    16681 cagtatgaat gataacgggc tggatactgg cgagcaggca aaagctttcg cattgggaaa
    16741 agtccgcgac gcgcttagtc aacaggttaa tcagcacgta gagtcctggc tatcaccgtg
    16801 gggaaatgcc agtgttgacg tcaaagtgga taacgaagga catttcaccg gcagtcgtgg
    16861 aagctggttt gtgccgttac aagataatga tcgttatctc acctggagcc agcttggtct
    16921 tactcagcag gataatgggt tggtgagcaa tgtgggcgtt gggcaacgct gggcgcgcgg
    16981 caactggctg gtgggttata acacttttta tgacaacttg caggacgaaa atcttcagcg
    17041 agcgggcttt ggtgccgaag cgtggggcga atatttgcga ttatcggcaa acttttatca
    17101 gccgtttgct gcatggcatg aacagacagc cacgcaggaa caacggatgg cgcgcgggta
    17161 cgacctgaca gctcggatgc gcatgccgtt ctatcaacac ctcaatacca gtgtcagcct
    17221 agaacagtat tttggtgatc gtgttgattt gtttaactct ggtacgggtt atcacaatcc
    17281 cgtcgcgttg agtctgggat taaattacac ccctgtgcca ttagtcactg tgacggccca
    17341 gcataaacag ggggaaagtg gcgaaaatca aaataacctc gggctgaatc ttaattaccg
    17401 ctttggtgta ccgctcaaaa aacaactttc tgcgggcgag gttgccgaaa gtcagtcgtt
    17461 acgtggtagt cgctatgaca atccgcagcg aaataatcta ccgactcttg agtaccgaca
    17521 gcgaaaaacg ttaacggtgt ttctggcgac accgccgtgg gatctaaaac ctggcgaaac
    17581 agtgccgctg aaattacaaa tccgcagtcg ttacggtatt cggcaactga tttggcaggg
    17641 cgatacgcag atattaagtt tgacgccggg cgcacaagcc aacagcgcgg agggctggac
    17701 gctgatcatg cctgactggc agaacgggga aggggcaagc aatcactggc gattgtctgt
    17761 ggtggtggaa gataaccagg ggcagcgtgt ctcctccaat gagatcacgc taacgcttgt
    17821 cgaaccgttc gatgcattgt caaacgacga actgcgctgg gaaccgtaat cagaaaatgc
    17881 gctcctgatg cacccatacc gctgcttcca cgcgagactt gagcttcatt ttcttcagca
    17941 tgtgcttgac gtgcactttt actgtgcttt cggtgatatc caggcggcgg gcaatcatct
    18001 tgttcggcaa accctgggca atcagcttga gaatatcgcg ctcgcgtggg gttaactggt
    18061 taacatcgcg ctcagtagtg gcacggttag cgcgcaagct ggcggccaga acaggcgtta
    18121 atgcttcgct taataccatt tcgccagcag ctgcctgatg caatgctttc agcagatctt
    18181 ccggttccat atcttttaac agatagccat ccgcgccgcg tttcagtgcg gtgaccacat
    18241 cttcttcatg gttagagacg ctgaatacca caatgcgccc tgagagggac ttttcgcgca
    18301 gtttatccag cgtttccaga ccgttcatgc cgggcatatt gagatctaac aggatcagat
    18361 cgggatcaag agattccgcc agttcaatac cctgttcgcc attactcgct tcgccaacca
    18421 cggtgatatc tggtgccata ctgataagct gttttacgcc agttcgcagc atcgggtgat
    18481 cgtcaatcag caggatagta gccggttcct gattactcat gggtatctcc ttggacgtct
    18541 gtgaaagttt tttcgggaat aaaggtgacc accacttcgg tgccacctga ttcacgacgg
    18601 cggacgcggc aatcgcctcg taaactttgc gcacggtcgc gcattattat catgccgtag
    18661 tgattgctgc ggatggcatt ttcaggcacg ccacagccgt tatcctggac ggtcagtttg
    18721 acctgattat cgttttgcgc caccgtcacc acgacctcac tcgcttgcga atgtttgagg
    18781 gcgttactta atgcctcacg ggcaatttgc aacaagtgga ttgcctgatg tgaaggcacc
    18841 agacgaggcg gcaattgata atccagcttc accgggaagc caaatttggc gctgtactct
    18901 tcgcaactcg cttccagcgc cggacgtaat ccaggctcgg tgagctgcaa gcggaatgtg
    18961 gtgagcaatt cacgcaactg cgcccaggat gcattcagtt cgttacggat ctgacttaac
    19021 agttcgcggc tgctttctgg cagcgcatcg ccctgcatct gtaaacaact cacctgcatc
    19081 ttcatgcaag agagagattg ggcaatagaa tcatgcagtt cgcgcgcaat ggtggcacgc
    19141 tcttccatca cgatcaactg ttgctgacgt tcctgatggc gatccagcgc cagcgtggcg
    19201 gtaagttgtt caaccagggt atccaccagt tgttgttggt catggctaag atgacgcccc
    19261 tgcggcaggg tcgccagcaa aataccgtac tgcgtatgag agtcagccag ccgccacttc
    19321 agggtcgtgc cgcgatcacc aacgggtaat acgccgcgcg ggcagagctg gcagccttta
    19381 tcatcacaag tcatatctgg ctggcaggta aactcctgat gattctcttc atcatcagtg
    19441 tcatacaccc gcagttcgat atcacgtagc agggttaaat tctgtaagcc gttgagtacc
    19501 ggtgacaggc gttcacacag cggggcgcgg gaatgcaaac ggcggttagc ctgccataaa
    19561 aaagagagga tctgattttt atgctccagc ccggcggttt tctcctgaac ccgctgctca
    19621 agtacggcat aactttcggc cagttctgca gacatattgt tcaacgcagt tccaagcatc
    19681 gccatttcgt tgcgcccgct gatgtttgcg cgttgggtaa aatcgcgatg gctgacggca
    19741 ctcgccattg ccagcagttg ccgccacggt tgtagcagtc gcgcccgcaa ccagataata
    19801 gtgaacacca gtaaaagtgc cataaatacc gccattaccc gatggaccag taccactgtc
    19861 tcaatacgca tttccgtggt gcggtcaaaa ccagatacca gctgatcaag cccggcaaca
    19921 aactggctga catccgctga caccgtttct cggttttgtg cacgcatcag cgcagggatc
    19981 agttcattac gccagtaatc ttgtaagccc tgtaattgcg ccagttgtcc gtctcgttct
    20041 gctgctcgag tcaactcggc gctaaatgcc gtttgttcca tctctttaat taagggcttg
    20101 tctttctcgc ttaatggcac tgccgccaac agacggtaac tttgcatgcg cagcgatccc
    20161 gctttgttga tcgcatgggc gctgccctga acgccttgca ccagccagcc agaaaccgcc
    20221 atccctgcca gtccaatagc agtagaaagc aacacaataa gcgcaacctg attaaccagg
    20281 gtgagcggag agagacaacg tttaagcatg taaacccctt ccttcaggct ttaaatgagc
    20341 aataacctta atgaatgtga cgatatattc tggaatggca gtattctcgg ctattggctg
    20401 aagtataccc atagccggaa agagttactc cttatttgcc gtgtggttag tcgctttaca
    20461 tcggtaaggg tagggattct acagcaccgt gaaaaatctc ataattttta tgaaatcact
    20521 gtactcacta tgggtaatga taaatatcaa tgatagataa agttatctta tcgtttgatt
    20581 tacatcaaat tgcctttagc tccagacact aaggtggcag acatcgaaac gagtatcaga
    20641 ggtgtctatg agtcactcat ctgcctccga aagggctact ggagctgtca taacagattg
    20701 gcgaccggaa gatcctgcgt tctggcaaca acgcggtcaa cgtattgcca gccgcaacct
    20761 gtggatttcc gttccctgtc tgctgctggc gttttgcgta tggatgttgt tcagcgctgt
    20821 tgcggtgaac ttaccgaaag tcggttttaa ttttacgacc gatcagctat ttatgttgac
    20881 tgcgctgcct tcggtttctg gcgcgttatt acgtgttcca tactccttta tggttcctat
    20941 cttcggtggt cgtcgctgga cggcgttcag caccggtatt ctgattattc cttgcgtctg
    21001 gctgggtttt gccgtgcagg atacctccac gccttatagc gtcttcatca tcatctctct
    21061 gctatgcggc tttgctggcg cgaacttcgc atccagtatg gcaaacatca gcttcttctt
    21121 tccgaaacag aagcagggtg gcgcgctggg tctgaatggt ggtctgggaa acatgggcgt
    21181 cagcgtcatg cagttggttg ctccgctggt ggtatcactg tcgattttcg cagtatttgg
    21241 tagccagggc gtcaaacagc cggatgggac tgagctgtat ctggcgaatg cgtcctggat
    21301 atgggtgccg ttccttgcca tcttcaccat tgcggcgtgg tttggcatga acgatcttgc
    21361 tacctcgaaa gcctccatca aggagcagtt gccggtactc aaacggggtc atctgtggat
    21421 tatgagcctg ctgtatctgg caaccttcgg ctccttcatc ggcttctccg cgggctttgc
    21481 gatgctgtca aaaacgcagt tcccggatgt tcagattctg caatacgctt tcttcgggcc
    21541 gtttattggt gcgctggcgc gttctgcagg tggtgcatta tctgaccgtc tgggcggaac
    21601 tcgtgtcacg ctggtgaact ttattctgat ggcgattttc agcggcctgc tgttcctgac
    21661 cttaccgact gacgggcagg gcggaagctt catggcgttc ttcgcggtct tcctggcgct
    21721 gttcctgaca gctgggctgg gtagtggttc cactttccag atgatttcag tgatcttccg
    21781 taaactgaca atggatcgcg tgaaagcaga agggggttct gacgaacgtg cgatgcgtga
    21841 agcggcaacc ggcacggcag cggcgctggg tttcatctct gcgattggcg cgattggtgg
    21901 cttctttatc ccgaaagcgt ttggtagctc gctggcatta acgggttcgc cagtaggtgc
    21961 aatgaaagta tttttgattt tctatatcgc ctgcgtagtg attacttggg cggtatatgg
    22021 tcggcattct aaaaaataaa tcttaatatt tgattatcct ttgcgcggca tgatgccgcg
    22081 ctttttttat gcgtcattta gttacaacat attagtgtta tatggcttat ttcgccggat
    22141 ttcattaaga gccattaata tgttaccc
//
LOCUS       DAZPCS010000051        21942 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZPCS010000051 DAZPCS010000000
VERSION     DAZPCS010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21942
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.051"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..157)
                     /locus_tag="ACHUQ7_001941"
     CDS             complement(<1..157)
                     /locus_tag="ACHUQ7_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFR1135373.1"
                     /translation="MLARDKSNLKIEEIRMHKHHEIHRVKPLMPALCRIRQGKKVINW
                     ETHSLTVD"
     gene            complement(167..718)
                     /gene="ybcL"
                     /locus_tag="ACHUQ7_001942"
     CDS             complement(167..718)
                     /gene="ybcL"
                     /locus_tag="ACHUQ7_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HFR1135374.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(887..1219)
                     /gene="emrE"
                     /locus_tag="ACHUQ7_001943"
     CDS             complement(887..1219)
                     /gene="emrE"
                     /locus_tag="ACHUQ7_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HFR1135375.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(1564..1878)
                     /gene="fliE"
                     /locus_tag="ACHUQ7_001944"
     CDS             complement(1564..1878)
                     /gene="fliE"
                     /locus_tag="ACHUQ7_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFR1135376.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            2092..3751
                     /gene="fliF"
                     /locus_tag="ACHUQ7_001945"
                     /pseudo
     CDS             2092..3751
                     /gene="fliF"
                     /locus_tag="ACHUQ7_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
     gene            3744..4739
                     /gene="fliG"
                     /locus_tag="ACHUQ7_001946"
     CDS             3744..4739
                     /gene="fliG"
                     /locus_tag="ACHUQ7_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFR1135377.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLRRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLAKRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            4732..5418
                     /gene="fliH"
                     /locus_tag="ACHUQ7_001947"
     CDS             4732..5418
                     /gene="fliH"
                     /locus_tag="ACHUQ7_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFR1135378.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            5418..6791
                     /gene="fliI"
                     /locus_tag="ACHUQ7_001948"
     CDS             5418..6791
                     /gene="fliI"
                     /locus_tag="ACHUQ7_001948"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFR1135379.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            6813..7154
                     /gene="fliJ"
                     /locus_tag="ACHUQ7_001949"
                     /pseudo
     CDS             6813..7154
                     /gene="fliJ"
                     /locus_tag="ACHUQ7_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
     gene            7151..8278
                     /locus_tag="ACHUQ7_001950"
     CDS             7151..8278
                     /locus_tag="ACHUQ7_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFR1135380.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            8383..8847
                     /gene="fliL"
                     /locus_tag="ACHUQ7_001951"
     CDS             8383..8847
                     /gene="fliL"
                     /locus_tag="ACHUQ7_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFR1135381.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            8852..9856
                     /gene="fliM"
                     /locus_tag="ACHUQ7_001952"
     CDS             8852..9856
                     /gene="fliM"
                     /locus_tag="ACHUQ7_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFR1135382.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASISGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVEFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            9853..10266
                     /gene="fliN"
                     /locus_tag="ACHUQ7_001953"
     CDS             9853..10266
                     /gene="fliN"
                     /locus_tag="ACHUQ7_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFR1135383.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            10269..10634
                     /gene="fliO"
                     /locus_tag="ACHUQ7_001954"
     CDS             10269..10634
                     /gene="fliO"
                     /locus_tag="ACHUQ7_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFR1135384.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            10634..11399
                     /gene="fliP"
                     /locus_tag="ACHUQ7_001955"
                     /pseudo
     CDS             10634..11399
                     /gene="fliP"
                     /locus_tag="ACHUQ7_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly.; frameshifted; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
     gene            11409..11678
                     /gene="fliQ"
                     /locus_tag="ACHUQ7_001956"
     CDS             11409..11678
                     /gene="fliQ"
                     /locus_tag="ACHUQ7_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFR1135385.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            11687..12472
                     /gene="fliR"
                     /locus_tag="ACHUQ7_001957"
     CDS             11687..12472
                     /gene="fliR"
                     /locus_tag="ACHUQ7_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFR1135386.1"
                     /translation="MMQETSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPGSHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     SEPLNSNAFLAPTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            12757..13381
                     /gene="rcsA"
                     /locus_tag="ACHUQ7_001958"
                     /pseudo
     CDS             12757..13381
                     /gene="rcsA"
                     /locus_tag="ACHUQ7_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
     gene            complement(13425..13613)
                     /gene="dsrB"
                     /locus_tag="ACHUQ7_001959"
     CDS             complement(13425..13613)
                     /gene="dsrB"
                     /locus_tag="ACHUQ7_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFR1135387.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVALEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            13776..13954
                     /locus_tag="ACHUQ7_001960"
                     /pseudo
     CDS             13776..13954
                     /locus_tag="ACHUQ7_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2525 domain-containing protein"
     gene            14252..15067
                     /locus_tag="ACHUQ7_001961"
                     /pseudo
     CDS             14252..15067
                     /locus_tag="ACHUQ7_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(15064..16758)
                     /gene="dgcQ"
                     /locus_tag="ACHUQ7_001962"
                     /pseudo
     CDS             complement(15064..16758)
                     /gene="dgcQ"
                     /locus_tag="ACHUQ7_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
     gene            complement(16929..17111)
                     /locus_tag="ACHUQ7_001963"
     CDS             complement(16929..17111)
                     /locus_tag="ACHUQ7_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFR1135388.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(17190..18107)
                     /locus_tag="ACHUQ7_001964"
     CDS             complement(17190..18107)
                     /locus_tag="ACHUQ7_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFR1135389.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            18280..19200
                     /gene="yedA"
                     /locus_tag="ACHUQ7_001965"
     CDS             18280..19200
                     /gene="yedA"
                     /locus_tag="ACHUQ7_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HFR1135390.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     QAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(19189..19659)
                     /gene="vsr"
                     /locus_tag="ACHUQ7_001966"
     CDS             complement(19189..19659)
                     /gene="vsr"
                     /locus_tag="ACHUQ7_001966"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFR1135391.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(19640..21058)
                     /gene="dcm"
                     /locus_tag="ACHUQ7_001967"
     CDS             complement(19640..21058)
                     /gene="dcm"
                     /locus_tag="ACHUQ7_001967"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFR1135392.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(21125..21626)
                     /locus_tag="ACHUQ7_001968"
                     /pseudo
     CDS             complement(21125..21626)
                     /locus_tag="ACHUQ7_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
     gene            complement(21705..>21942)
                     /gene="drpB"
                     /locus_tag="ACHUQ7_001969"
     CDS             complement(21705..>21942)
                     /gene="drpB"
                     /locus_tag="ACHUQ7_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein DrpB"
                     /protein_id="HFR1135393.1"
                     /translation="WAFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWL
                     GALVGFLFMALVGALLGGIAWYTRPRSARCRRYE"
BASE COUNT         5375 a         5790 c         5575 g         5202 t
ORIGIN      
        1 tatcaacagt tagactatgc gtctcccaat tgataacttt ttttccctga cggatacgac
       61 ataaagctgg cataagaggc ttaaccctgt gaatctcatg atgtttatgc atccgtattt
      121 cttcaatctt taggttagac ttatctcttg ccagcatact cttaccctat tttatctcat
      181 aaactggtgt tatctcagcg gttgcaattt tattggcatt aagcatataa ccaactaacg
      241 ctccgctgga attagaatcc acaggaatct tatcagtttt tagagcccat actttaaact
      301 ggtaatgatg gggtttatct cctttgggag gacatgctcc accaaatcct gcataaccaa
      361 aatcatttcg gccttgaaca gcaccagtcg gaagttttgt accatcacgt cttcctgcat
      421 cagtgggcaa atatgttaca gttgctggaa tattagcaac agtccagtgc caccatccac
      481 tgcctgtagg agcatctgga tcatacacag ttacggcaaa gcttttggtg ccttcaggag
      541 caccagacca ggttaatgag ggcgatgtat taccaccttc acacccaaat ccagaaaata
      601 catgagacgt tgtaagttgc tctcctgttt ttatttcatt actagtgacc tgaaatgctg
      661 cggcctgcgc agaaaatgtt atgaatgcca atacacttga aacgataagt tttttcataa
      721 aaacctcatt gttgtgacct atcgttattt tatttgatgt gccttgatct cattatgcat
      781 aaaggcgcaa tgatcgcgca aatgcaatca taattataaa ggtgccccga acattagcca
      841 taatatgcac aaatcagatt cctgttatct caaggagaat tattttttaa tgcggtgcac
      901 ttcgtgacaa caagttaatg actaacacac cggcacaaat caacatcatg cctatgatag
      961 ctggcaggtc cagtcgttga ccaaaaaatc cccaggaaag caaactaatc agaacgatac
     1021 cgactcctga ccagatagca taagcaatcc ctgtaggtat ataagccagc gtctgagcta
     1081 ataaccagaa tgatgcacaa tagcaaataa ttgtgccaac agagggccat aaccgtgtaa
     1141 acccctctga aaacttcatt aaggttgtac caatgacctc tgcaagtatt gcgccaccaa
     1201 gataaatata agggttcata gcatattctt tcctgttcat actggagaga attgtactac
     1261 agtttgaaca caactcaccg ttttcatcct ggtgtatgtg tatccaaggg gctctggcgt
     1321 ggcaagagat aaaggcagga acaataatga tcagaaggaa ccccaggcta acgaatgagt
     1381 tcactcttcc tgggagcaaa gcgaagacaa aacatgttat cgcgtttacg gatcgtggat
     1441 ggcagaaaac aaccaggatc aggtatccat tttaaaccag aaattgagtg agtttgcccc
     1501 atccatgccc cacgctgtgg gatcggatat aattaaacaa gcatagtcaa caggttacac
     1561 aacctacacc tgcatggaca tcacttcctg atacgccgcc accagcttat tacgcacctg
     1621 aatccccatt tgcatagaaa ctgaggcttt ttgcatatcg gtcatcacat cgtttagcgc
     1681 cacgccgggt tcaccgaggg tgaatttttc tgcctgcgtg cgggcgactg tttgtgtatc
     1741 gcttatgcga tcgagcgcgg cgtgcagctg cccggcaaaa ctaatggtcg gttgcggcag
     1801 tgattcctgc gcacgcgcac tcatcgccgt agcctgtaac tggctgataa ccccttcaat
     1861 cccctgtatc gctgacattt tcgtctcccg gataatttct ggtaacaaag cctaccagta
     1921 agtcaataag acaaaggcgc taaatagcaa caaaaaaact ggtttattgg cggatagaaa
     1981 aaacgaaagc acaaataatg ggagcgtcaa tttttcgagt gtgctgaccc gagagtgagt
     2041 cttgttccac tttgccaata acgccgtcca taatcagcca cgaagtgcgc gatgaatgcg
     2101 actgcagccc agacaaaatc tcttgagtgg cttaatcgcc tgcgtgcgaa tccgaaaatt
     2161 ccattgattg ttaccggttc cgcggcagtg gcggtcatgg tcgcactgat cctgtgggcg
     2221 aaagcccccg actaccgcac attattcagc aatctttccg atcaggatgg tggcgcaatt
     2281 gtcagccaac tgacgcaaat gaatattcct taccgcttca gcgaagccag cggcgctatt
     2341 gaggttccgg cagataaagt tcacgaactg cgcctggcgc aacaaggttt gcccaaaggc
     2401 ggcgcggtcg gtttcgaact gctcgatcag gaaaagtttg gtatcagcca gttcagcgaa
     2461 caggtgaatt atcagcgggc gctggaaggc gagctttctc gtaccatcga aactatcggc
     2521 ccggtaaaag gggcgcgcgt acatctggca atgccgaaac cgtctttatt cgtccgtgaa
     2581 caaaaatccc cttctgcatc ggtgacggta aatctgttac ccggccgcgc actcgatgaa
     2641 gggcaaatta gcgctattgt acatctggtt tccagcgcgg ttgctggtct gccgcctgga
     2701 aacgtcacgc tggtggatca gggcgggcat ctgttaaccc agtccaatac cagcgggcgc
     2761 gatcttaatg acgctcagtt gaaatatgcc agcgatgtcg aaggccgtat tcagcagcgt
     2821 attgaagcga tcctgtcacc tattgttggt aacggtaata ttcacgccta ggtcacggcg
     2881 caactggact tcgccagtaa agaacaaacg gaagaacagt atcgccctaa cggtgatgaa
     2941 tctcatgcgg cgcttcgttc acgccagctt aatgagagcg agcaaagcgg ttccggttat
     3001 ccgggcggcg taccgggggc gttgtcgaat caaccggcac ctgcgaataa cgcgccaatc
     3061 agcacgcctc cgacaaatca aaataaccgc cagcagcagg cgagcaccac cagcaatagt
     3121 gggccgcgta gcacacagcg gaatgaaacc agtaactacg aagtcgatcg caccattcgt
     3181 cataccaaaa tgaacgtggg cgatgtgcaa cgtctgtcag tcgcggtagt ggtgaattac
     3241 aaaaccttgc cagacggtaa accattgcct ctcagcaacg aacagatgaa gcaaattgaa
     3301 gctctgaccc gcgaggcgat gggcttttct gaaaaacgcg gtgattcgct caatgttgtt
     3361 aactcgccgt tcaatagcag tgacgaaagc ggcggagcac tgccgttttg gcaacagcaa
     3421 gtgtttatcg atcagttgct ggctgccggt cgctggttgc tggttgctgg tactgctggt
     3481 ggcgtggttg ctgtggcgga aagcggtacg cccgcagcta acacgtcgcg ccgaggcggt
     3541 gaagactgtg cagcaacagg cgcaggcccg cgaggaagtg gaagatgcgg tggaagtccg
     3601 tctgagcaaa gacgaacaac tccaacaacg gcgcgctaac caacgactgg gggcagaagt
     3661 catgagccag cgtatccgtg aaatgtctga taacgatccg cgcgtggtgg cgctggtcat
     3721 tcgccagtgg ataaataacg atcatgagta acctgacagg caccgataaa agcgtcatcc
     3781 tgctgatgac cattggcgaa gaccgggcgg cagaggtgtt caagcacctc tcccagcgcg
     3841 aagtgcaaac cctgagcgct gcaatggcga acgtcacgca gatctccaac aagcagctaa
     3901 ccgatgtgct ggcggagttt gagcaagaag ctgaacagtt tgctgcactg aatatcaacg
     3961 ccaacgatta tctgcgttcg gtattggtca aagctctggg tgaagaacgt gccgccagcc
     4021 tgctggaaga tattctcgaa acccgcgata ccgccagcgg tattgaaacg ctcaacttta
     4081 tggagccgca gagcgccgcc gatctgattc gcgatgagca tccgcaaatt atcgccacca
     4141 ttctggtgca tctgaggcgc gcccaggccg ccgatattct ggcgttgttc gatgaacgtc
     4201 tgcgccacga cgtgatgttg cgtatcgcca cctttggcgg cgtgcagcca gccgcgctgg
     4261 ccgaactgac cgaagtactg aatggcttgc tcgacggtca gaatctcaag cgcagcaaaa
     4321 tgggcggcgt gagaacggca gccgaaatta tcaacctgat gaaaacgcag caggaagaag
     4381 ccgttattac cgccgtgcgt gaattcgacg gcgagctggc gcagaaaatc atcgacgaga
     4441 tgttcctgtt cgagaatctg gtggatgtcg atgatcgcag cattcagcgt ctgttgcagg
     4501 aagtggattc cgaatcgctg ctgatcgcgc tgaaaggggc cgagcagcca ctgcgcgaga
     4561 aattcctgcg caatatgtcg cagcgtgccg ccgatattct gcgcgacgat ctcgccaagc
     4621 gtggtccggt gcgtctgtcg caggtcgaaa acgaacagaa agcgattctg ctgattgtgc
     4681 gccgtcttgc cgaaactggc gagatggtga ttggcagcgg cgaggatacc tatgtctgat
     4741 aatctgccgt ggaaaacctg gacgccggac gatctcgcgc caccaccggc agagtttgtg
     4801 ccaatggtcg agtcggaaga aaccattatt gaagaggccg aacccagcct tgagcagcaa
     4861 ctggcgcaac tgcaaatgca ggcccacgag cagggttatc aggcggggat tgccgaaggt
     4921 cgccagcaag gtcacgaaca gggctatcag gaaggactgg cccaggggct ggaacaaggt
     4981 ctggcagagg cgaaggctca acaagcgcca attcatgccc ggatgcagca actggtcagc
     5041 gaatttcaaa ctacccttga tgcgcttgat agtgtgatag cttcgcgcct gatgcagatg
     5101 gcgctggagg cagcacgtca ggtgatcggt cagacaccaa cggtggataa ctcggcactg
     5161 atcaaacaga tccaacagtt gttgcagcaa gaaccgttat tcagcgggaa accacagctg
     5221 cgcgtacacc cggatgatct gcaacgtgtg gatgatatgc tcggcgcaac tttaagtttg
     5281 catggctggc gcttgcgggg cgatcccacc ctccaccctg gcggatgtaa agtctccgcc
     5341 gatgaaggcg atctcgacgc cagtgtcgcc actcgctggc aagaactctg ccgtctggca
     5401 gcaccaggag tggtgtaatg accacgcgcc tgactcgctg gctaaccacg ctggataact
     5461 ttgaagccaa aatggcgcag ttgcctgcgg tacgtcgcta cgggcgatta acccgcgcta
     5521 ccgggctggt gctggaagcc accggattac aattgccgct cggcgcaacc tgtgtcattg
     5581 agcgccagaa cggcagcgaa acgcacgaag tagaaagcga agtcgttggc tttaacggtc
     5641 aacggctgtt tttaatgccg ctggaggaag tggaaggtgt cctgcccggc gcgcgtgtat
     5701 atgccaaaaa catttcggca gaagggctgc aaagcggcaa gcagttgccg ctcggtccgg
     5761 cgttattagg tcgtgttctg gacggtagcg gtaaaccgct cgatggcctg cccttccccg
     5821 atacgacgga aaccggtgcg ctgattaccc cgccatttaa cccgttgcaa cgtacaccga
     5881 ttgaacatgt gctggacacc ggcgtgcgcc caatcaatgc cctgcttacc gtcggacgcg
     5941 ggcagcgtat ggggctgttt gccgggtcgg gcgttggtaa aagtgtgctg ctggggatga
     6001 tggcacgcta cacccgcgcc gatgtcattg tcgtgggttt gattggtgaa cgtgggcgcg
     6061 aagtaaaaga ttttattgag aatatcctcg gtgccgaagg acgtgcacgc tcagtggtga
     6121 ttgccgctcc ggcggatgtt tctccgctcc tgcgaatgca aggtgccgcc tatgccacgc
     6181 gcattgccga agattttcgc gatcgtggtc agcatgtgtt gctgattatg gactccctca
     6241 cccgctacgc gatggcccag cgtgaaattg cgctggcgat tggcgaacca cctgccacta
     6301 aaggttatcc accgtcggtg tttgccaaat taccggcact ggtcgagcgt gccggtaatg
     6361 gcattagcgg cggcggctcg attaccgcgt tttataccgt gctcaccgaa ggcgatgacc
     6421 agcaggaccc cattgctgac tccgcgcggg caatcctcga cggtcacatt gtgctgtctc
     6481 gccgactggc ggaagccggg cactatccgg ctatcgatat tgaagcgtcg atcagtcgcg
     6541 caatgacggc gttgatcagt gagcaacatt acgcgcgagt gcgcaccttc aaacagctgt
     6601 tgtcgagttt tcagcgtaac cgcgatctgg ttagcgtcgg cgcgtatgcc aaaggcagcg
     6661 atccgatgct cgataaagcc atcgccctgt ggccgcagct ggagggctat ttgcaacaag
     6721 gcatttttga acgcgcggac tgggaagcgt ctctccaggg gctggagcgt attttcccga
     6781 cagtgtcata acccaggaga taacggcaga tggcggaaga acagctcaaa atgttgatcg
     6841 attatcagaa tgaatatcgc aataacctca atagcgatat gagtgccggg atgaccagca
     6901 accgctggat caactatcag cagtttatcc agacgctgga aaaagccatt actcagcatc
     6961 gccagcaact taatcagtgg acgcagaaag ttgacattgc cctgaacagc tggcgagaaa
     7021 agaaacaacg tttgcaggcc tggcagacac tgcaggaacg gcaatccacg gcggcactgc
     7081 ttgcagaaaa ccgcctcgat cagaaaaaga tggatgagtt cgcccagcgc gccgccatga
     7141 ggaaacctga atgattcgct tagcgccctt gattaccgcc gacgttgaca ccaccacatt
     7201 gcctggcggc aaagccagcg atgctgcaca agattttctc acgttgttga gcgaagcatt
     7261 agcaggcgag acaacaactg acaaagcggc cccccagttg ctggtagcaa cagataagcc
     7321 cacgacaaaa ggcgagccgc tggtcagcga tattcttgcc gatgcgcaac aagcggattt
     7381 actgatccct gtggatgaaa cacttcctgt catcaacgac gaacaatcca catcaacacc
     7441 gttaaccacc gctcagacga tgacgttggc tgcggtggct gacaaaaata cgacaaaaga
     7501 cgaaaaagcg gatgatctta atgaagacgt caccgcaagt ctgagcgccc tttttgcgat
     7561 gttgccgggt tttgacaata cgcccaaagt gactgatgca ccgtcaaccg tgttaccggc
     7621 agagaaacca acgctcttca caaaactgac ttctgcgcaa ctcactacag cacagcctga
     7681 tgacgccccc ggcacaccag ctcagccatt aacaccgctg gtagcagaag cccagagtaa
     7741 agcggaagtc atcagcacgc cttcgccggt gaccgctgac gccagcccgc taatcactcc
     7801 acaccagaca cagccactgc ccaccgtcgc cgcgcctgtt ttgagtgcac cgctgggttc
     7861 tcacgaatgg caacaatcat tgagccagca tatttcgctg ttcacccgcc aggggcaaca
     7921 aagtgcagag ttgcgtctgc acccacagga tttaggtgaa gtgcaaattt ccctcaaagt
     7981 ggatgataac caggcgcaga tccagatgat ttcaccgcat cagcacgtac gcgccgccct
     8041 ggaagcagcg ctgccggtac tgcgtacgca gctggccgaa agtggcattc agttagggca
     8101 aagcaacatt agtggcgaaa gctttagtgg tcagcagcag gccgcttccc agcaacagca
     8161 aagccaacgc acagcaaacc atgaacctct ggcgggggaa gacgacgata cgcttccggt
     8221 tcccgtctct ttacaagggc gtgtaacagg taacagcggc gttgatattt tcgcctaacg
     8281 tcagaggtag caccgtaatc cgcgtctttt ccccgctttg ttgcgctcaa gacgcaggat
     8341 aattagccga taagcagtag cgacacagga agaccgcaac acatgactga ttacgcgata
     8401 agcaagaaaa gcaagcgatc gctttggatc ccgattctgg tattcattac cctcgcggcc
     8461 tgtgccagcg caggttacag ctactggcat tcgcatcagg ttgccgctga cgacaaagcg
     8521 cagcaacgcg tcgtgccctc cccggtcttc tatgcgctgg atacctttac ggtcaatttg
     8581 ggcgatgcgg atcgcgtgct ttatatcggc ataaccctgc gactgaaaga tgaagcaacc
     8641 cgctcgcggc tgagtgagta tttgccggaa gtccgtagtc gcttgctgtt actgttttca
     8701 cgtcaggatg ctgccgtact ggcgacagaa gaaggcaaga aaaacctgat tgccgagatt
     8761 aaaaccacac tttccacccc gcttgttgcc gggcaaccga aacaggatgt caccgacgtg
     8821 ctgtataccg cttttattct gcgataacga catgggcgat agtattcttt ctcaagctga
     8881 aattgatgcg ctgttgaatg gtgacagcga agtcaaagac gaaccgacag ccagtattag
     8941 cggcgaaagt gacattcgtc cgtacgatcc gaatacccaa cgacgggttg tgcgcgaacg
     9001 tttgcaggcg ctggaaatca ttaatgagcg ctttgcccgc cattttcgta tggggctgtt
     9061 caacctgctg cgccgtagcc cggacattac cgtcggggcc atccgcattc agccgtacca
     9121 tgaatttgcc cgcaacctgc cggtgccgac caacctgaac ctaatccacc tgaaaccgct
     9181 acgcggcaca gggctggtag tgttctcgcc gagtctggtg tttatcgccg tggataacct
     9241 gtttggcggc gatggacgct tcccgaccaa agtggaaggt cgcgagttta cccatactga
     9301 acagcgcgtc atcaaccgta tgttgaagct ggcgctggaa ggctatagcg acgcctggaa
     9361 agcgattaat ccgctggaag ttgagtacgt gcgttcggaa atgcaggtgg aatttaccaa
     9421 tatcaccacc tcgccgaacg acattgtggt taacacgccg ttccatgtgg agattggcaa
     9481 cctgaccggc gaatttaata tctgcctgcc attcagcatg atcgagccgc tacgggaatt
     9541 gttggttaac ccgccgctgg aaaactcgcg taatgaagat cagaactggc gcgataacct
     9601 ggtgcgccag gtgcagcatt cacagctgga actggtggcc aactttgccg atatctcgct
     9661 acgcctgtcg cagattttaa aactgaaacc cggcgacgtc ctgccgatag aaaaacccga
     9721 tcgcatcatc gcccatgttg acggcgtccc ggttctgacc agtcagtacg gtactctcaa
     9781 cggtcagtat gcgttacgga tagaacattt gattaacccg attttaaatt ctctgaacga
     9841 ggaacagccc aaatgagtga catgaataat ccggccgatg acaacaacgg cgcaatggac
     9901 gatctgtggg ctgaagcgtt gagcgagcaa aaatcaacca gcgaaaaaag cgctgccgac
     9961 gcggtgttcc agcaatttgg cggtggtgat gtcagcggaa cgttgcagga tatcgacctg
    10021 attatggata ttccggtcaa gctgaccgtc gagctgggcc gtacgcggat gaccatcaaa
    10081 gagctgttgc gtctgacgca agggtcagtc gtggcgctgg acggtctggc gggcgaaccg
    10141 ctggatattc tgatcaacgg ttatttaatc gcccagggcg aagtggtggt tgttgccgat
    10201 aaatatggcg tgcggatcac cgatatcatt accccgtccg agcggatgcg ccgcctgagc
    10261 cgttagtgat gaataaccat gctactgtgc aatcttccgc gccggtttct gctgagccac
    10321 tgctgcaggt gagcggcgca ctcatcgcca ttattgccct gatcctcgct gctgcctggc
    10381 tggtaaaacg gctgggattt gcccctaaac gcactggcgt taacggtctg aaaattagcg
    10441 ccagtgcttc gctgggcgcg cgtgaacggg tcgtggtggt cgatgtggaa gatgcacggc
    10501 tggtgctcgg cgttaccgca gggcaaatca atctgctgca taaacttccc ccttcagcac
    10561 caacggaaga gataccgcag accgattttc agtcggtcat gaaaaatttg cttaagcgtg
    10621 gcgggagatc ctgatgcgtc gtttgttttc tgtcgcaccg gtccttctct ggctgattac
    10681 gcccctcgcc ttcgcgcaac tgccgggtat caccagccag ccgctacctg gcgggtatca
    10741 ccagccagcc gctacctggc ggtggacaaa gctggtcgct cccggtgcag acgctggtgt
    10801 tcatcacctc gttgacgttc attccagcga ttttactgat gatgaccagt ttcacccgca
    10861 tcattattgt ttttggttta ttgcgtaacg cgctgggaac gccctccgcg ccacctaacc
    10921 aggtattgct ggggctggca ctgtttttga ccttttttat tatgtcgcca gtgatcgaca
    10981 aaatttatgt cgatgcgtac cagccattca gcgaagagaa aatctcaatg caggaggcgc
    11041 tggaaaaagg ggcgcagccg ctgcgtgagt ttatgctgcg tcagacccgc gaggcagatt
    11101 tagggttgtt tgccagactg gcgaataccg gcccgttgca ggggcctgaa gctgtgccga
    11161 tgcgcatttt gctcccggcc tacgtgacca gcgagttgaa aaccgcattt cagataggct
    11221 tcacgatttt catccctttt ttgattatcg acctggtgat tgccagcgtg ttgatggcat
    11281 tggggatgat gatggttccc cccgccacca ttgctctgcc ctttaaactg atgctgtttg
    11341 tactggtgga tggctggcaa ttgctggtcg gttcgctggc gcagagcttt tacagctaga
    11401 gaggcaaaat gacacctgaa tcggtcatga tgatgggaac tgaagcgatg aaagtcgcgc
    11461 tggcactggc agccccgctg ttgctggtag cgctggtcac gggccttatc atcagtattt
    11521 tgcaggcagc cacgcagatt aacgaaatga cgctgtcgtt tattccgaaa atcatcgccg
    11581 tatttatcgc catcattatt gccggaccgt ggatgctcaa tctgttgctg gattacgtcc
    11641 gcaccttgtt cactaacctg ccgtatatca tcgggtagcc cgtacaatga tgcaggagac
    11701 aagcgatcaa tggctttcct ggttaagcct gtacttctgg ccgttactgc gcgtgctggc
    11761 gctcatttct accgcgccaa ttctgagcga acgcagcgtg ccgaaacggg taaaactggg
    11821 tctggcaatg atgatcacgt tcgccattgc cccatcatta cctgccaacg atgttcctgt
    11881 tttttcgttc tttgctctgt ggctggccgt acagcagatc ctgatcggca ttgcgcttgg
    11941 ttttaccatg caatttgcct ttgccgctgt gcgaaccgct ggcgaaatta tcggtctgca
    12001 aatggggctg tcatttgcga catttgtcga tcctggcagc catctcaata tgcccgtttt
    12061 agcgcgcatc atggatatgc tggcattact gctgttcctg acatttaacg gtcatttatg
    12121 gttgatttca ctgctggtcg atacctttca caccctgccg attggtagcg aaccgttgaa
    12181 cagcaatgcg tttctggcac ccaccaaagc agggagtttg attttcctta acgggctgat
    12241 gctggcgtta ccgctcatta ctctgttgct gaccctgaat ctggcattag gtttactgaa
    12301 tcgtatggcc ccgcaattat ccatttttgt tattggattt ccattaactc tgactgtcgg
    12361 catctcttta atggcggcat taatgccgtt aattgcgcct ttttgcgaac atttattcag
    12421 tgaaattttt aatttgctgg ctgatattat tagtgaatta ccattaatat aattccgtaa
    12481 cgtttatcat gctaccctaa ggattatccg aaaaataata tctgcgaaca tcttccagga
    12541 tactcctgta gcgaaatatt tgttttaagc tcactcacat atcgcaacat ttactttaag
    12601 acaattccag gcaaattata caacacttta cgggatagta agtccgcctg aaaaattgcg
    12661 agagtggcgc attaggtgac ccatgttgtt ccgtttagtc atgatgaaat attcaggtaa
    12721 ggggaattat cgttacgcat tgagtgaggg tatgccatgt caacgattat tatggattta
    12781 tgtagttaca cccgactagg tttaaccggg tatctgttga gtagaggggt taaaaaaaga
    12841 gaaatcaacg acattgaaac cgttgatgac cttgccatag cttgtgattc acagcgtcct
    12901 tcagtggtgt ttattaatga ggactgtttc atccacgatg cttctaacag tcagcgcatc
    12961 aagcacatca ttaatcaaca tcccaatacg ttatttatcg tttttatggc aattgccaat
    13021 gttcattttg atgaatatct attggtcaga aaaaaattta ttgatcagtt ctaaatcgat
    13081 taaaccggaa tctctcgacg atatccttgg cgatattctg aaaaaagaga caacgataac
    13141 ctcgttttta aatatgccga cgttatcatt gagccgaacc gaatcgagta tgttgcgaat
    13201 gtggatggca ggtcagggaa ccattcaaat ctccgaccaa atgaatatca aagccaagac
    13261 cgtttcatcg cataaaggta atattaaacg taagatcaaa acgcataata aacaggttat
    13321 ctaccatgtc gtccgactga cggataatgt gactaatggt atttttgtca acatgcgcta
    13381 acacattctg actggtggtt tcccaccagt caggctgaat aagattactc tgctttctca
    13441 acaaagatac cgtcctgatg ccctgcttca ttaaagaacc agatgccgag cgggtagtct
    13501 tccagcgcaa ccaggtacat tgtgccttca ctaaactcct caactgccag taccactcca
    13561 ggacgacgcg gaccgccatc cgttttgact gttacccgat cattcacctt catcgttttc
    13621 ctcctgtggc tttgtgccag tgtagaacaa tttcgttttt ctggcagcgc caagcgcgcg
    13681 cgagtgctga ttttctcgac ggtctatact taagagatgc caggcggact taacgactgg
    13741 cggcaacaac agagtaacgg ttgcgaggaa agatgatgaa aaccgcaaaa gtgtacagcg
    13801 ataccgcaaa acgtgaggtc agcgtcgatg tcgccgccag aacgattctg aacacgttag
    13861 cgtcgatgga cgtgaatatc atacatggcg tgaattggcg gatgccttcg aactggatat
    13921 tcatgacttc agcgtctctg aagtgaatcg ttgaatgcac aataaaaaaa tcccgaccct
    13981 gagggtgtcg ggatgaaact tgcttaagca agaagcactt aaaaaattcg ttacaccagg
    14041 aaatctgatg tgttcatcac cttatctgca atttttttcg ctgacaagaa aatattcgcg
    14101 ctatgaatga ttaacgctac ttatgaatag aaatgtgacg cgcgtcaccc cttggtggct
    14161 ctggtgtcaa cgggatcgct cccgaaaaaa tcatctttga ttatttgtgc tgttttgaga
    14221 atcatcctgg taaggggagt actgcgcgac catgttttca attcaacaac cactactggt
    14281 ttttagcgat cttgatggca ccctgctgga cagtcatagt tatgactggc aaccggcagc
    14341 cccctggctc agccgtttac acgaagccaa tattcccgtc attctctgta gcagtaaaac
    14401 tttagcggaa atgctgtact tgcaaaaaat gttggggcta caaggcttac cgctgattgc
    14461 agagaatggc gcagtgatcc agcttgctga acaatggcag gatatagacg gttttccacg
    14521 catcatctca ggtattagcc atggcgaaat ctgccaggtt ttaaatacgc tacgcgagaa
    14581 agaacatttt aaattcacga cttttgatga tgtcgacgat gcaaccattg ccgaatggac
    14641 gggattaagc cgtagctagg cggcgctgac gcagctgcat gaggcgtcgg taacgctaat
    14701 ctggcgcgac agtgacgagc gtatggcaca atttatcgct cgtctgaacg aactgggctt
    14761 gcagtttatg cagggtgcgc gcttctggca cgtactggat gcctctgccg gaaaagatca
    14821 ggctgccaac tggattatcg cgacctatca acaattgtca ggcagacgcc caaccacact
    14881 tggcctgggc gatgggccaa acgatgcgcc tttactggag gtaatggatt acgcggtgat
    14941 tgtgaaaggg ctaaaccgtg aaggggtaca tctgcatgat gaggatccgg cccgcgtctg
    15001 gcgaacgcag cgtgaaggac cggaaggctg gcgtgaaggg ctggaccatt ttttctccgc
    15061 ccgttaagcg ttatcgctcg cgaatacccg attgcgcccg gcctgttttg cgaggtaaag
    15121 ccgacggtcg gccaatgact ggagttgttc aaaatcataa tcaccggttt cctcgctgct
    15181 acttaccccc agcgaggcac tgatgcgtat cgttgtactc ttggcgatca gcatctcttt
    15241 ttcatttaac ttcaggcgaa tacgttctgc gacctccgca gcctgcgtca gattcgcgcc
    15301 tggcagaatc acacaaaact cctcaccacc gacccgcccg gcaacgtcct gcgcacgcaa
    15361 ggaactgcta attaatccgg cagcatgaga aagaacacgg tcgcccgcct gatgaccaaa
    15421 gcggtcatta atcgctttaa aatggtcaag atcgacctga atgacagaaa aaggatgttg
    15481 gtgcgtctga cacaatttag cgagcggacg ggctttttcg aacagtgcgc cacggttata
    15541 taaacgcgtt aaggtgtcgt gccacgcctg ccactgcaac gagctttgca gaacatacat
    15601 gttgctgacc atccggcgaa tcacatacca ggagaggagt aacatggtgg taaagagcgc
    15661 ccacagcagg gttaatgcaa tgctgatact gccgaaatcg ccgcgcacgc cttcgcttag
    15721 cgtatggacg cgcgccagca caccgtcgaa atggtccaga cgttcccagc taacatagcg
    15781 actgtccata cgaatgccgc cccgcgtgtc atgttccatt gcctgcgcca gcaaggccag
    15841 ttcacgagga tcaaaaatat tccctgttgg atggtcagga ttggaagagg tcaaaaatct
    15901 cagcttactg tcatagagct gatactcacc atcgaggttt ttatcgatgg cgtttcttaa
    15961 aaattgctgc atagtacgca cgggaatact catccccagc acgccatacc agtagttatt
    16021 actgtctacc ggaacactga cggtaacctg cggttcagta ttgctggcgt gttccggttg
    16081 cgaagtgaac cagcgtaccg cgcggtgacg attttctcgt tgcgaatggc cgataaacca
    16141 gggttgggtg acatagccgt aataacgcgt tggtacattg cgcgtaaaca aggtcggctg
    16201 cgtcgaaacg taaaatccag cacgcgagac atacatcgcc tgttcaacca tcgacgagga
    16261 gttgtgcgcc agtcgcagca agtaaccaac ttccagtgca gcggtaattt cattgtcgag
    16321 gctttcattt tcgcgagaca ggaaattccc ctcgctgact aatgcatccg atacgccatt
    16381 gactggcagg gtgcgccgtc ggttgagttc gatttgccag gcgtgctcgt cgcgatgctg
    16441 ttcgaactcg gttacggcat tacgcagaga ggtgaaatcc agtggcgcca cgagagcttc
    16501 gcgcatgcca ttacgcaaaa agatcagttt gtcgacgtta tactgcaaat gcttatcgag
    16561 cgcgttggct acgttttcca gatgattacg ctagctggag atataggcat cttccagcac
    16621 caccacttca cgccaggtga gcaaggtgga aaaaagcaat accacgataa agcagagatt
    16681 aacgacatga ccgggcccca ggcggcgtgc gagttttttc aaccagctct ggttttccat
    16741 ttttgtctcg tgctgcaccc tgacgactcc caacctgctt ttttatgatt ctggcataac
    16801 ccggcaatag cgtaccgcaa aacagcgata attttataat ggtggcatga taagacgcga
    16861 ctatcattca ttgcaggcat tgggacacca ggccggatgt gatccggcct ggtgttaatg
    16921 ataccctttc aaacttcttc cgcagaacga ctcccctccc ccggaacaag atcggtttca
    16981 tgaaaagctt cccgcttgac cccgtaaccg tcataccaac gacactcaac cataccgctg
    17041 gagtatccag tgacaatcat ccgcgggccg ccctctttaa ccgtaacttc ctcactaacc
    17101 ataaagctca tactcgcctc cttttttcga gtgaaacttg ttcaccttag ttgaagatgg
    17161 cgaattttgc ataatagcca gtgcgagtat taatgtgcct gaccgcgcat tttcgctact
    17221 gctttcactc ccagcaccac gatgccgccg atgataaatc caagaatcag atttaaaaca
    17281 gtcggtaata tcatcgccac cactgcactt tgctgcccgg cgatatgttc aatagcgtga
    17341 tgcagcggcg caataccatg taccacaatc ccgccgccga cgaggaacat cgccagcgtg
    17401 ccgacaatcg ataatgcttt catcagccaa ggcgcgataa tcaataaccc tttacctaat
    17461 gcctgcatca gcgcgctaga tttttccgcc agccaatacc ctaggtcatc aatcttaacg
    17521 ataaccccta ccagaccgta gacgcccacg gtcactacca gcgcgatgcc tgaaagcacc
    17581 agcacctgat taagcaacgg cgcctctgcc acaatcccca gcgtgatggc gacgatttcc
    17641 gcagacaaaa taaaatcggt acgaatcgcc cctttaattt tgtccttttc aaacttcagc
    17701 ggatcctgcg ccgccagctt ctccagacgc tgctggctct gcgctggatc ttctttatgt
    17761 ttacgcgcct ccagcatatg cagcactttc tctacccctt caaagcagag aaacgcgcca
    17821 ccaatcatca acagtggcgt aatcgcccac gggataaacg cactgatgat cagcgccagc
    17881 ggcaccagaa tcactttatt gatcagcgat cctttcgcca cgccccagac cacgggaagt
    17941 tcccggttgg cccgcacgcc tgaaacttgt tgcgcattga gcgataaatc atcccctaat
    18001 acaccggcgg ttttcttcgc cgccagtttg cccatcacag agatatcgtc cagcagtgtg
    18061 gcgatatcat cgagcaacgt cagtaaacta ctcccggcca aaagcattcc ctctcatttt
    18121 tattgttgaa gggtgtaagt atgaagcaaa aacggcagct ctgatacttg cttcacaggt
    18181 caacatttat ttaacaatca agtgcaatta tatctctcgc cagcagaatg attttcacgt
    18241 ttactatgaa ccaccttttt atttccgccg tgagggatta tgcgtttccg ccagttgtta
    18301 ccgctttttg gcgcgctgtt tgcgttgtat atcatttggg gctcaaccta ttttgtcatt
    18361 cggattggcg tgggaagctg gcctccgtta atgatggcgg gcgttcgatt ccaggcagcc
    18421 ggtattttat tgctggcatt tttgctactg cgcggacaca aacttccccc gctacgtccg
    18481 ctgctcaatg ccgcgctgat tggcctgtta ttgctggctg tcggtaatgg catggtgacg
    18541 gttgccgaac atcaaaatgt tccttccggc atcgccgccg tagtgattgc aaccgtgccc
    18601 ctctttaccc tgtgcttcag tcgcctgttt ggcattaaaa cgcgcaaact ggaatgggtg
    18661 ggtattgcca ttgggcttgc cggaatcatc atgctcaata gcggtggaaa tttaagcggc
    18721 aatccgtggg gcgcgattct gattttaatc ggctcgatta gctgggcgtt tggctcagtt
    18781 tatggctcgc gcattacctt acctgtaggg atgatggcgg gtgcgattga gatgctggcg
    18841 gcaggcgtgg tgttaatgat cgcatcgatg attgcgggtg aaaaactgac ggcgctccct
    18901 tccctgtcag gcttccttgc ggtcggctat ctggcgctgt ttggttcgat tatcgccatt
    18961 aacgcttata tgtatttaat ccgtaatgtc agtccggctc tcgccaccag ctacgcctac
    19021 gttaacccgg tggtcgcggt cttgctgggt acgggactgg gtggcgaaac gctgtcgaag
    19081 attgaatggc tggcgctcgg cgtaattgtt ttcgcggtgg tactggtcac gttgggaaaa
    19141 tatctcttcc cggccaaacc cgttgtcgcg ccagttattc aggatgcatc aagcgagtaa
    19201 atgaatcccc tgcgtgtcga tctgcgcgct ggcaccttcg ccgcagatcc actcttccag
    19261 acgctcggta agcgccgcat ccgtcagctt ctcgcgccta cgtaacgcac actcccagac
    19321 aatcagtacg cgccagccga gctcctgcaa gcgactgata tcgcggcgat cgcgttcaac
    19381 atttttacct atcttctcca gccagaactc agttcgagtc gcaggcactt taaacagata
    19441 gcagtgatga tgatgccaga agcagccgtg ggtaaatatc acgcagcgat attcatcaac
    19501 gacaaaatcc gggcgtccgg gcagactggc gtcctgaacg cgaaacgcaa ggccctgccc
    19561 ggttaacaaa ctggcgaggc gcttctcaat cgccgtatca cgcgtggcaa tcgcgcgcat
    19621 atttttgctg cgggtggcct tatcgtgaac gtcgaccatg ttgtgcctct tgctgacgca
    19681 acgccaccgc ctgtttgatt tttggctcaa gcagttttgc caccgcggca aagaccggca
    19741 cgaccaccga gttgccgaac tggcgatagg cctgagtgtc cgaaaccgga atgcggaatt
    19801 tcgcttctcc cggcgcttca aaacccatta agcgcgcgca ttcacgaggc gttaaccgac
    19861 gtggacgatg ttgctgattc agcggatcgt caaagtcttt ctcacccgtg gccatatccc
    19921 aaccgcgatc gattaaaatt tccgcgccat ctttgtaata acgagcagac agcgtacggg
    19981 tgacgctttg cggattgttc ggataaacca ttccataacc gaagccgtta ccgcgcgcct
    20041 gatgtttttt cgcatatcga tagaggtact tccacagcac cggcgtcagg atatatttcg
    20101 cctcaaccat cgggtccagc agctgcgcca gcgtcactcg ctgtgcaggg aaacattcgc
    20161 tgatatcacg cagagtaaaa tcggctttca gattcagatc gcgacgaaaa cccaccagca
    20221 cgatgcgttc acggtgctgc ggcaggaaat gtttaccatc gatgattttc ggatcgtccg
    20281 gcccgttatc ttctgcatcg gcaacgtcat agcccagttc gtccagcgtc tgcatgatga
    20341 tgcggaacgt ttttccctta tcgtgactct tcaggttttt gacgttttcg agcacaaaca
    20401 tcgccggacg acgcgcgtcg ataatgcgca ccacatcaaa gaacagcgtg ccctgggtat
    20461 cgcaggcaaa accgtgcgcc cgcccgagcg agttcttttt cgatacgcca gccagcgaaa
    20521 atggctgaca agggaaaccg gccagtaaaa catcgtgttc aggaatgtgt tgacgaatat
    20581 gttccgccgc cgcctcatca ctcacgcctt cttgatgact gagggtgatg tcgcggatat
    20641 cttcattaaa atgatgcgtc gccgggtcgc aataatggtt ggctttataa gtgcgtaccg
    20701 catgtttgtt ccattcgctg gtaaacacgc actgtccacc aatcgattca aaaccacgac
    20761 ggatgccgcc aattccggca aatagatcga taaagcgaaa cgcataatgc ggatgatgtt
    20821 ccggtggttt gggtaacaac gtttgcagat gggcaaactc tttctcactt aaacggtgcc
    20881 atgccgagtc attcgccagc gcacgtttta aaattgccgc gctccagtga ttctcaccca
    20941 caccattaag ctgcgccacc agcattttaa catcataaat ttgcagcagt ttctccagca
    21001 ttgcctgtgc ggcattcccg gtgctgtatg aatcggttac tgatatattt tcctgcatag
    21061 atttaaccgg ccatctgaga tggccgacag gttaacataa ttactgccgt ttagggccac
    21121 gcgctcagcc agcgggtgaa aacgcatcta tcaccttgtg atcgatacct tcattctccc
    21181 ccgccagttc ggcaccgagc ttcgccatca aacagcgcca agctcaatgc ccctgaaacg
    21241 gcaaatacgc gcgccaggcc aatcgccccc agggcttcca gccgatccgc atcctggacg
    21301 attttagctt ccgttgttaa gggggcgatt tgcgcgctga aactgtgagc ggcaatggca
    21361 tgacaaacgg cctcaatttt ctccgccgga aattgcacga actcttcacg gagcaggcga
    21421 cgcgtctctt ctgccgccag gattgaagaa cgctgccgtt gcggatgatt tttcgccagg
    21481 ctcacgatat cgtgaaaata acaggcggtt aaaatcacca gcatatcgac atcgtcatct
    21541 gccgccagtt tctgtgccgt agcccagaca cggcgaaaat gacacaggtc atgcgccgcg
    21601 tcctgatgtt gatgatggtt tttcaaccag ttctcgaact atgcctgcca gtgttgtaac
    21661 tccatacgcg ctccgttgcc aaagagtctc aaccttagca gtttttattc atagcgtctg
    21721 caacgtgcgg aacgaggtcg ggtgtaccat gcaatgccgc ctaacaaggc accaaccagg
    21781 gccataaaca gaaatccgac caatgctcct aaccacttcc ccgcggtaga tcctaaatca
    21841 ctttcaaatc ccgacacggg ggcatcgggg atccggctca tcacccagat gtatcgcgcc
    21901 attgcccaga cgaaatagcc acaccatgta tagaaagccc ac
//
LOCUS       DAZPCS010000052        21512 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZPCS010000052 DAZPCS010000000
VERSION     DAZPCS010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21512
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.052"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(19..1017)
                     /gene="fbp"
                     /locus_tag="ACHUQ7_001970"
     CDS             complement(19..1017)
                     /gene="fbp"
                     /locus_tag="ACHUQ7_001970"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFR1135394.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            1193..2566
                     /gene="mpl"
                     /locus_tag="ACHUQ7_001971"
     CDS             1193..2566
                     /gene="mpl"
                     /locus_tag="ACHUQ7_001971"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFR1135395.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2649..3200)
                     /gene="yjgA"
                     /locus_tag="ACHUQ7_001972"
     CDS             complement(2649..3200)
                     /gene="yjgA"
                     /locus_tag="ACHUQ7_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFR1135396.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3294..4646
                     /gene="pmbA"
                     /locus_tag="ACHUQ7_001973"
     CDS             3294..4646
                     /gene="pmbA"
                     /locus_tag="ACHUQ7_001973"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFR1135397.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4829..5211
                     /gene="cybC"
                     /locus_tag="ACHUQ7_001974"
                     /pseudo
     CDS             4829..5211
                     /gene="cybC"
                     /locus_tag="ACHUQ7_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
     gene            complement(5256..5720)
                     /gene="nrdG"
                     /locus_tag="ACHUQ7_001975"
     CDS             complement(5256..5720)
                     /gene="nrdG"
                     /locus_tag="ACHUQ7_001975"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFR1135398.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5878..8016)
                     /gene="nrdD"
                     /locus_tag="ACHUQ7_001976"
     CDS             complement(5878..8016)
                     /gene="nrdD"
                     /locus_tag="ACHUQ7_001976"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFR1135399.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVNEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVNVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFSGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8410..10065)
                     /gene="treC"
                     /locus_tag="ACHUQ7_001977"
     CDS             complement(8410..10065)
                     /gene="treC"
                     /locus_tag="ACHUQ7_001977"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFR1135400.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(10115..11536)
                     /gene="treB"
                     /locus_tag="ACHUQ7_001978"
     CDS             complement(10115..11536)
                     /gene="treB"
                     /locus_tag="ACHUQ7_001978"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFR1135401.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(11655..12602)
                     /gene="treR"
                     /locus_tag="ACHUQ7_001979"
     CDS             complement(11655..12602)
                     /gene="treR"
                     /locus_tag="ACHUQ7_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HFR1135402.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12787..12840
                     /gene="mgtL"
                     /locus_tag="ACHUQ7_001980"
     CDS             12787..12840
                     /gene="mgtL"
                     /locus_tag="ACHUQ7_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFR1135403.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12981..15677
                     /gene="mgtA"
                     /locus_tag="ACHUQ7_001981"
     CDS             12981..15677
                     /gene="mgtA"
                     /locus_tag="ACHUQ7_001981"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFR1135404.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKLTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFRSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15883..16269)
                     /gene="ridA"
                     /locus_tag="ACHUQ7_001982"
     CDS             complement(15883..16269)
                     /gene="ridA"
                     /locus_tag="ACHUQ7_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFR1135405.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16342..16803)
                     /gene="pyrI"
                     /locus_tag="ACHUQ7_001983"
     CDS             complement(16342..16803)
                     /gene="pyrI"
                     /locus_tag="ACHUQ7_001983"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFR1135406.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEEQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16816..17751)
                     /gene="pyrB"
                     /locus_tag="ACHUQ7_001984"
     CDS             complement(16816..17751)
                     /gene="pyrB"
                     /locus_tag="ACHUQ7_001984"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFR1135407.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17755..17889)
                     /gene="pyrL"
                     /locus_tag="ACHUQ7_001985"
     CDS             complement(17755..17889)
                     /gene="pyrL"
                     /locus_tag="ACHUQ7_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFR1135408.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
     gene            complement(18170..18565)
                     /locus_tag="ACHUQ7_001986"
     CDS             complement(18170..18565)
                     /locus_tag="ACHUQ7_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFR1135409.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQSEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(18697..19410)
                     /gene="bdcA"
                     /locus_tag="ACHUQ7_001987"
     CDS             complement(18697..19410)
                     /gene="bdcA"
                     /locus_tag="ACHUQ7_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR1135410.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            19481..20074
                     /locus_tag="ACHUQ7_001988"
     CDS             19481..20074
                     /locus_tag="ACHUQ7_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFR1135411.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            20219..20671
                     /gene="tabA"
                     /locus_tag="ACHUQ7_001989"
     CDS             20219..20671
                     /gene="tabA"
                     /locus_tag="ACHUQ7_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFR1135412.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            20794..>21512
                     /locus_tag="ACHUQ7_001990"
     CDS             20794..>21512
                     /locus_tag="ACHUQ7_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFR1135413.1"
                     /translation="MGKISDLNYSQHITLADNFKQKNEALDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIEFSSYSKDTDGNEKMKF
                     YMNNPEGERADLSKVKIEITLASASTKGIREGHTVIIFKQSDGSTNRYEGKSFERKDD
                     SSLHLITNKVLACYQREANKKIARLLNNHQKLNNIQELNDSQELNNSQKLNNSQELNN
                     SQVSCKGSVDSTITDLLEKAL"
BASE COUNT         5195 a         5324 c         5669 g         5324 t
ORIGIN      
        1 gtggagaggc gcaggagatt acgcgtccgg gaactcacgg ataaagcgtt cgacatcttc
       61 aaccatatgg tcgttgccga caaagaacga acggcgctgg tgcagagttt ccgggatgat
      121 atccagaata cgctctttgc catcgctcgc tttaccgccc gcttgttccg ccaggaatgc
      181 catcgggttg cactcataaa gcaaacgcag tttgccgtcc gggtggctgg cggtgcttgg
      241 gtagagataa ataccgcctt tcagcaggtt acggtggaaa tccgcaacca gtgaaccgat
      301 ataacgcgag gtatacgggc gattggtgga tttatcttct tcctggcaga atttaatgta
      361 cttcttcacc ccgttcggaa acttaatgta gtttccttcg ttgatggagt aggttttgcc
      421 tttctccggg aagcgcatcc gttcctggca caggcagaaa acgcccagcg aaggatcgta
      481 agtaaaggcg tgaacaccgc atccggtggt gtaaaccagc atggtagagg agccgtatac
      541 cacgtaacct gccgcaacct gtttgttacc aggctggagg aaatcttctt ccgttaccgg
      601 cgtgccaaca ggcgtaacgc ggcggtagat ggagaaaatg gtaccgacag agacgttaac
      661 atcgatgttg gacgagccat ccagggggtc catcagcacc acgtattttg cgtgttcaca
      721 gccttcaaag acgacaatct catcttcttc ttcagaggca atccccgcaa cgatatcgcg
      781 tgctttcagt gcggctttca gtttttcatt agcgaacaag tcgagttttt gctgaacctc
      841 gccctgcacg ttctcagcac cgctggcacc caggatatca accagtcctg ctttgttgat
      901 atcgcgatgg ataatcttgg cgcccagttt tattgccgac agcaaagcag tgagctcacc
      961 ggtagcatga gaaaactcgt gctgcttttc gacaataaat tcacctaacg ttttcataaa
     1021 actttccctg caatgtttat ggagtaaagc gaccgcaaca atcttaacaa ataatctcaa
     1081 tgttgcgctc aggtgaatcg cgccagcaaa ttgcggatta tcctgaaatg cgtttctcac
     1141 ttgcccgaca tatgcgtaaa atgagcggca gattaaaaaa ggatagtgac gtatgcgcat
     1201 tcatatttta ggaatttgtg gcacatttat gggcggtctg gcgatgctgg cgcgccagtt
     1261 aggccatgaa gtaacgggtt cggacgccaa tgtgtatccg ccgatgagca ccttacttga
     1321 gaagcaaggc attgagctga ttcagggtta cgatgccagc cagctcgagc cgcagccgga
     1381 tctggtgatt attggcaacg ccatgacccg tggaaatccg tgtgtggaag cggtactgga
     1441 aaaaaacatc ccttatatgt caggtccaca gtggctgcac gattttgtgc tgcgcgaccg
     1501 ctgggtgctg gccgttgccg gtacacacgg caaaaccacc accgcgggaa tggcgacctg
     1561 gattctggaa cagtgtggtt acaaaccggg atttgtgatc ggcggtgtgc cggggaactt
     1621 tgaggtttcg gcgcgtctgg gcgaaagcga cttctttgtt atcgaagcgg atgagtacga
     1681 ctgcgccttc ttcgacaaac gctctaaatt tgtccattac tgcccgcgta cgctgatcct
     1741 caacaacctt gagttcgatc acgccgatat ctttgacgac ctgaaagcga tccagaaaca
     1801 gttccaccat ctggtgcgta tcgttccggg gcagggccgt attatctggc cggaaaatga
     1861 catcaacctg aaacagacca tggcgatggg ctgctggagc gagcaggagc tggtgggtga
     1921 gcagggtcac tggcaggcga aaaagctgac caccgatgct tccgaatggg aagttttgct
     1981 ggatggcgaa aaagtgggcg aagtgaaatg gtcgctggta ggcgaacata atatgcacaa
     2041 tggcctgatg gcgattgcag cggctcgcca tgttggtgta gcgccggcag atgccgctaa
     2101 cgcgctgggt tcgtttatta atgctcgtcg ccgtctggag ttgcgtggtg aagcgaatgg
     2161 cgtcacggta tatgacgatt ttgcccatca cccgacggcg attctggcaa cgctggcggc
     2221 gctgcgtggc aaagttggtg gtacggcgcg cattattgct gtgctggaac cgcgctcgaa
     2281 taccatgaaa atggggatct gcaaagacga tctggcacct tcattaggtc gtgctgatga
     2341 agtcttcctg ctgcaaccgg cgcatattcc gtggcaggtg gcagaagtgg cagaagcctg
     2401 cgttcagcct gcacactgga gtggcgatgt ggatacgctg gcagatatgg tggtgaaaac
     2461 cgctcagcct ggcgaccata ttctggtgat gagcaacggc ggttttggtg ggatccatca
     2521 gaaactgctg gatggtctgg cgaagaaggc ggaagctgcg cagtaattcg gcttcagcct
     2581 gatatagcat tgccggataa ggcgtttacg ccgcatccgg caaaccatac tcacatcaac
     2641 aacgaaaatt acccttcatt ctccgccagt tcgcgcagat actggaaaat ctggcgtgcg
     2701 gatttcggcg gcttattccc ttctttctct ttcttcgcgt tgcggatcag tgtacgcagt
     2761 tgctgacgat cggcatcggg ccacagattc aatacttcag cgatggcgtc atcaccttga
     2821 tcgatcagac gatcgcgcag gttttccagt ttatggaaca gcaccacctg ctggttgtga
     2881 cggtttttca gcttatccag cgcctgacga ataggctcta cgtcgcgctg gcgcagcatc
     2941 ttaccgatga gctgcaactg gcggcggcga ccttccatct taatacgctg ggcgagctca
     3001 atagccgggc gcagatccgc atctaacggg atcttatcca gcgcgttttt ccccagatca
     3061 acaatttccg cgccaaggcg ttttagctcc tcggcatcac gtttaatttc acttttactg
     3121 acccagataa tttcatcgtc ttcgtcttcg atgtcatcac cgggaacgtc gtcgagccag
     3181 tcttcgggct gcttagtcat ctcaggctcc ttaaaaaaag aggctaatgt taccagttaa
     3241 gatgcgcact gaaaaacggt tctctgttag acttcagaga aactctctac attatggcac
     3301 ttgcaatgaa agtaatctct caagttgaag cgcagcgcaa gattctggaa gaagcagttt
     3361 ccactgcgct ggagttggcc tcaggcaaat cggacggtgc ggaagttgcc gtcagcaaga
     3421 ccaccggcat tagcgtaagc acgcgttatg gtgaagtgga gaatgttgaa ttcaatagtg
     3481 atggcgcgct ggggatcact gtttatcacc agaaccgtaa aggtagcgca tcgtctaccg
     3541 atttaagccc gcaggccatt gcccgcaccg tacaggcggc gctggatatt gcccgttata
     3601 cctcgccaga tccctgtgcc ggcgtggcag acaaagagct gctggccttt gacgcaccga
     3661 atctcgactt gttccaccct gcggaagttt ccccggatga agccattgaa ctggcggccc
     3721 gcgcagaaca ggcggcattg caggcggaca aacgcatcac caataccgaa ggtggcagct
     3781 ttaacagcca ctacggtgtc aaagtttttg gcaacagcca cggcatgttg cagggttact
     3841 gctcaacgcg tcattcgctc tccagctgtg taattgccga agaaaatggc gatatggagc
     3901 gtgattacgc ctacaccatt ggccgcgcga tgagcgatct gcaaacgcca gagtgggtcg
     3961 gggccgactg tgctcgccgt actttatcgc gcctgtcacc gcgtaaactc tccaccatga
     4021 aagcgccggt catttttgcc aatgaagtgg caaccgggct ttttggccat ctggtggggg
     4081 cgatagcggg tggctcggtt taccgtaaat ctacctttct gctggattcg ctgggtaaac
     4141 aaattctgcc ggactggctg accattgaag agcatccgca tctgctgaaa gggctggcgt
     4201 cgacgccatt cgacagcgaa ggtgtgcgca ccgagcgtcg cgatattatt aaagatggca
     4261 tcctgactca gtggctgctg accagctact cggcgcggaa actggggctg aaaagcaccg
     4321 gacatgcggg cggtattcac aactggcgga ttgccggaca agggctaagc ttcgaacaaa
     4381 tgctcaaaga gatgggcacc gggctggtgg tgacggagct gatgggccag ggcgtgagtg
     4441 ccattaccgg tgattattcc cgtggtgcag cgggcttctg ggtagagaat ggcgaaattc
     4501 agtatcccgt tagtgaaatc accatcgcag gtaatttaaa agatatgtgg cgcaatattg
     4561 tcaccgtcgg taacgatatt gaaacacgca gtaatataca gtgtggttct gtgctgttgc
     4621 cggagatgaa aatcgccggg cagtaaaaaa ctggcgcgac ctgccgcgct ataaactaaa
     4681 aaattacaca aaatcattcg cactgcatcg agacggaaag tgaatgaggg ccggggagcg
     4741 tactcgcagt acgtgacccg ggcgaatgag cgtagccgtc gatgaggcag tgtgaaggat
     4801 gaagtgtaaa taaaaaagga agtgagcaat gcgtaaaagc ctgttagcta ttcttgcagt
     4861 ctcctcgttg gtattcagtt ctgcgtcgtt tgccgctgat cttgaagaca atatggaaac
     4921 cctcaacgac aatttaaaag tgatcgaaaa agcggataac gcggcgcaag tcaaagacgc
     4981 gttaacgaag atgcgcgccg cagcgctgga tgcgcaaaaa gcaacgccgc cgaagctcga
     5041 agataaatca ccggacagcc cggaaatgaa agatttccgc cacggtttcg acattctggt
     5101 cggtcagatt gacgacgcgc tgaagctggc aaatgaaggt aaaaagaagc gcaggctgct
     5161 gcagagcaac tgaaaacgac ccgcaacgcc tatcaccaga agtatcgtta attcctcatt
     5221 tccctgttgc ctgcactcag gtaacaggga acttatcatc gcaaatgatg caccacctga
     5281 ttgctgctgc cgcgccagat cagggaaggg tcttttaaat cctgcacaaa tttgccgtcg
     5341 accagcacgt taatcaaatc aacaacctgc atttgcgcag cgttgagttc gtcgagttta
     5401 tagcctgtcc acacccagat gtctttaccc ggacactcgg cgcggatgcg ttttaccagt
     5461 ttcagaatat ccggcacgtt ttgcggatgc agcggatcgc cgccggagag ggaaatcccc
     5521 tggcgtttga tacgagtgtc attcagatct ttaatgatct tgtcttccat tgctttggta
     5581 aatggctgac cagaatttac ccgccatgtg cttttgttat agcaaccggg gcattcatga
     5641 acacacccgg agacaaacag ggtgcagcga gtgccggggc cgttgacgat gtcgacagga
     5701 tagtactgat gataattcat tatttcgcct ggcttatcag tccgcgtgta ggctgcatcc
     5761 ggtaatcaat gcctgatgcg acgctgtcgc gtcttatcag gcctacagcc gttgccaaat
     5821 gtaggccgga taaggcgttt acgccgcatc cggcatttgc ttagcgcaga gtgaagatta
     5881 acctatctgc ccattcccca aatgtttaac gcggcgctta acttcttcct gcttaccagc
     5941 gttaaacgga cgtgcatccg ggctacctaa atatccgcac acgcggcgag ttaccgacac
     6001 acgggaggcg tcatggttac cacatttcgg gcaagtgaag cctttgctgg tgcactcgaa
     6061 ctcaccggta aagccacact cgtagcactc atcaatcggt gtattggtgc cgtaatacgg
     6121 tacatgctga tagctgtaat cccagacatc ttccagcgct ttcaggttgt gctgaatgtt
     6181 tggatactcg ccgtagcaaa tgaaaccacc gttcgccagc ggcgggtaag gcgcttcaaa
     6241 gtcgatcttg tcgtacgggt tcaccttctt ctccacatca aggtggaaac tgttggtgta
     6301 gtaaccttta tcggtcacgc ccggcaccac gccaaactca gcagtatcga gacggcagaa
     6361 gcggtcgcac aggttttcac tcggcgtgct gtaaagactg aaaccataac ccgtttcttc
     6421 tttccactga tccactgcct gacgcagacg ttcaacaatg gcgatacctt tcgcgcgaag
     6481 cttctcgttg tcgtaaacat gctcgccgct gaacagcgcg ttaatggttt cgtggatgcc
     6541 gatgtaaccc agcgaaatag acgcacgacc gtttttgaag atttcagaaa catcatcgtc
     6601 agcattcaga cgtacgccac aagcaccttc catatagagg atcggggcga cgcgcgcttt
     6661 cacgccttcg agacgagcga tacgggtcat cagtgcctta cgtgccagca ccagacgttc
     6721 atccagcagc ttccagaagg tggcttcatc gccttttgct tccagagcaa tacgcggcag
     6781 gttcaggctg atcacgccga ggttgttacg accatcgtgg atctgctcgc cgttttcatt
     6841 ttcccacacg ccgaggaagc tgcggcagcc catcggggtt ttaaacgaac cggtgacgtt
     6901 cactacctga tcgtagttca ggatatccgg atacatgcgc ttgcttgcgc actccagcgc
     6961 cagctgtttg atgtcgtagt tcggatcgcc ttttttatgg ttcaggccat cgcgaatcgc
     7021 aaacaccagt ttcgggaaca ccgcagtttt acggttttta ccgaggcctg cgatacggtt
     7081 acgcaggatc gattcctgaa tcaggcgcga ttcccagctg gtgcccagac caaaaccaaa
     7141 ggttacaaac ggcgtctgac cgttggcggt gtgcagggtg tttacttcgt actccagcga
     7201 ctggaaggca tcgtagcact ctttgatggt acgagagtta gcgtagcctt cggcgtccgg
     7261 gatgttccac tcttctgcgg tcttgcgatg tttgttgtag ctggcggtaa caaacggcgc
     7321 cagcacttca tcgatacggt taatggtggt gccgccataa atatggctgg cgacctgagc
     7381 aataatctgc gcagttaccg cggttgccgt agagatcgac ttcggcggtt caatctcggc
     7441 gttccccatt ttaaaaccct gggtcagcat gcctttcagg tcgatcaaca tgcagttgaa
     7501 catcgggaag aacggcgagt aatcgagatc gtgatagtga atatcgccac gctcatgtgc
     7561 ctgcaccacg tcacgcggca gcaggtgctg gcgtgcatag tgtttagcca cgatcccggc
     7621 cagcaggtcg cgctgggttg gaatcacctt gctgtctttg ttggcgtttt cgttgagtaa
     7681 cgaggcgttg gtctgctcga ccagaccacg gatctcctgg ttcaggcgac cgcgtttttc
     7741 acgttcaatg tcgcgatcgt gacggtactc gatgtaagca cgagccagtt gtttgtatgg
     7801 acccgacatc agctggtttt caactgcggt ctggatctcg ttgatatcca cctggttgcg
     7861 cccctgcatc tgctcgttga caaccgcggc aacagtggcg caataatcgg catcatcgac
     7921 ttccgctgct ttagctgcac gcagaatcgc ttctttgatg cgctctgatt taaacggcac
     7981 tttgcagccg tctcgtttca tcacatgcgg tgtcatgatc gctccatatt tttaagaaca
     8041 ggttatccac agaaattggg aaaggcgttt tccggttttt tcgtttcgtt ttccggtgct
     8101 ttccgcaatc gccatccgct tatccacaac gccgcacctt tttcgtgggg agctgtagta
     8161 gcaattatag tcgattaata caacatattg ggttgggacg cattttaaag tctatatata
     8221 gtgctttgca tcaaggatgt tttaactttt tttgatgtag ctcaaagtaa aaagcagagc
     8281 gtacggatga cgggcgctac agcgttatgt aaatttttta atgaatttgc tggttgaaaa
     8341 atcaacaaaa acaacatgct gacagacaaa accccggaat gaccggcatt atcggggctt
     8401 agggaagatt tacttctgta accaccagac agcctcaaaa ggccgtaaat tcatggcaca
     8461 gggttgtggt gaggcttctt cgtagttatg catcacaagc tgccagttgc cgcgcatttg
     8521 ccctggctgc cagggttgga tctcacggct aaggttggca atgaccagca aggtttgccc
     8581 cttccattca cggcgatagc accacaatac agggctgttt ggcagcagat cctggtaatt
     8641 gccccatgtc aggatggctt cttgcttacg cagtgcgatt aacttttggt aggtgtaaaa
     8701 cacagaggag tcatcggcca gcgcggcttc tacgttgatt tgttgatagt tatcgcctag
     8761 gccaatccac ggttcgccag ccgtaaaccc ggcattatcg ccgttgctcc attgcatggg
     8821 cgtgcggctg ttgtcacggg atttactggc gaggattgcc aataactcgt cggcatcacg
     8881 accatcgttg cgcagctcgg caaacatatt gaggctctcc acgtcgcgat agtcagtaat
     8941 gcgcgtgaaa tgcgggttgg tcatgccaat ctcttcgccc tggtagatat acggcgttcc
     9001 ctgcatgcca tgcagcacca tcgccagcat ttttgccgca ggcacgcggt attcaccttc
     9061 atcaccaaag cgagaaacaa tgcgcggctg atcgtggtta caccagaaca aggcattcca
     9121 tgccacgttg tgcattcctt gttgccagtg gcggaacaat gttttcaacg ccacaaagtc
     9181 aggtttagcc agcgtccatt tttcaccacc gggataatcg accttcaggt gatgaaaatt
     9241 aaaggtcatc gacaattcac tgcctgtcag tgccgcgtat cgctggcaat gctcaaggct
     9301 ggtggaggac atttcaccta cggtcattaa cccgcgtggc gtaaacacat cgcggttcat
     9361 ctcgtgcaaa aactcgtgtg ctcgtggccc gtcggtgtag aagcgacgcc cgtcgccatc
     9421 caggtcttca gggaaacgcg ggtctttgga gatcagattc accacatcca ggcgcaaccc
     9481 gtcgacccca cgatcggccc agaattcaca gacttttttc agctctgcgc gtaccgctgg
     9541 attctcccag ttgagatccg cctgttctgg tgcaaagaga tgcaaatagt actgttcgct
     9601 ttccgcatgc cagcgccacg cactaccgcc aaattttgaa cgccagttgt tcggtggcgt
     9661 ttctggttcg ccatcgcgcc agatataaaa ctggcggtaa gggctttctt tgttcagcgc
     9721 ctcgcgaaac caggcatgtt gggtagaggt atggttaaac accatatcga gaatgatacg
     9781 aatcccgcgc gattttgcct gcgtcaccag ttcgtcaaaa tcgtccagcg tgccgtaggt
     9841 gggatcaatc gccgtatagt tcgctacatc gtaaccgtta tcgacctggg gagagacata
     9901 aaagggggtt agccagatgg catcaacgcc cagtttatgc agatagtcca ggcgttggat
     9961 aacgccacgt aaatcgccgg tgccgctacc cgtggtgtcc tgaaaactct ttggataaat
    10021 ctggtagata acgccgtttt gccaccagtg gggaagatta gtcattacgt tattcctgca
    10081 aatgcgaggg ggcgcaattg cgccccgaag aaaattaaac aatgtccagc gtgcccaggc
    10141 ggtatttccg ctgatagata aacgaggtga gtacaatcgg gatgatgatg gcgatagcca
    10201 ttgccagcgc aaatacctgc cagtagctcg gttgaataga gagaattccc ggcaggcccc
    10261 ctacgccgat accattcgcc ataacgccgt tcaggccgca tagcaatcct gccagaccag
    10321 aaccaatcat cgcgcacagc atcgggaagc gatatttcag gttgatgccg tacattgcag
    10381 gctcagtgac cccaagccag gcggagatag cggcaggcac ggagatctcg cgttcattgt
    10441 gcttgcggct ggaaatgata atgcctatca cggcggagcc ctgagcgata ttcgacagcg
    10501 caatcagcgg ccacactggc gtgccaccca tgctttgaat catctgcaaa tcaatagcaa
    10561 gggtggtctg gtgtacgccg gtgatcacca gcggcgcgta caggaagcca aacaatgccg
    10621 caccaatcgg agcaaagctg ccggtcatca ggtgacgtac cgcaaaggca acgccatcgc
    10681 caatcatgcg accaaacgga ccaatcagcg catgggcgag gaacaccgcg aggatcagcg
    10741 aacagacggg caccaccacc agatagaggt aatccggtac gatgcgttta aggcgagttt
    10801 caataacgcc cagcgccagc ccggctaaca gtgccgggat cacctgcgcc tgatagccca
    10861 ctttggcgat gctgaacatg ccaaagtccc acacttccgg cagctgctgc ccgagcagat
    10921 aagcgttcat cagctggggg gaaaccagtg tcacgccaag cacgatacca aggatcggcg
    10981 tgccgcccat ttttttcacc gctgaccagc aaatcccgac cggcaggtag aagaaaatcg
    11041 cttcaccgat caaccacaga aaatcgtaga tcgtttgcag ggaagggtac atttgcgcca
    11101 gcgtctgacc gttgctcatg ggcaaatcgc cgatcacatt gcgaaaaccg aggatcaaac
    11161 cgccgctaat caacgcgggc agcaacggga agaagatctc cgcgaaatga gagatcaact
    11221 gctcatgcca tttcatattt tgccgggcgg ctttttttac ctgctcttta tcaacctgcg
    11281 cctgtccggt tgacgctatt agtgcttgat agtaatcacc cacgttggtg ccaatcacca
    11341 cctgaaattg cccggcattg gtgaaacagc ctttcaccat ggggagttgc tcaatttctt
    11401 tcggtctggc attggccggt tggttgagga caaagcgcag gcgagtaata cagtggctca
    11461 ccgtcgcaat attgccgcgc ccgccgacca gttcaatcaa ccgatcgata tccgtttggt
    11521 ttattttgct catcataaag ccccatggca gatgacattt ttggttggct gcagaatata
    11581 ttgcgcggaa aaatttaaaa acgggaacgt tcccgaaacg cagcgaagat cacaatttat
    11641 cgttcaggaa acgatcagga cagggtggcg gggatgatga tttgttgcgg ttcgctgcgc
    11701 ccggttacct gtgcgatcaa ttggcaggcc gcctggcgtc cagcttcggc gtagccagga
    11761 tctacggtga cgatctccgg atgaaggaat ttcattaacg gcgtattacc tacgctcgcc
    11821 agttgcaagg tgtcgatgcg ttgctcttgc aggtatttac ttgcgccaag agccagcgtg
    11881 tcggttgcgc acagtaacgc ggtagtttca ggcgtaatca cttttgctac gttttcatag
    11941 ccttgcttca tagcaagccc tggcagagcg gcaacgggat gcagtttatg cgctttgcag
    12001 aacgccaggt aggcttcgtg acgtcgcttc ccggtcgtca cgtcactatg cggcacgccg
    12061 agataactga tattgcgatg cccctggtca tagagccgtt gcatcagaat cttgattgcc
    12121 ccttcgtcgt cataacagac cgaagcaaag ccttttgcgt cacgcgccag cagaaccagc
    12181 gatgactgcc agtgggctaa catttcttcg gttatgccgg taaaaccgaa cagcactacg
    12241 ccgtcgatat tacgccgttt cagcaccccc aaatgttcgg caactaattg tggggaaaac
    12301 tgattttcca tcatgattgg atcgtaacct tgttcataga acgctggcag catggtttga
    12361 acggcgagat tttctgacaa cgaatccaga cgggtaacaa tgatggcgac cactttatcg
    12421 ctttgcccac gcatagcgcg cgcagagcgg gaaggggaaa atccatgctg attcatcact
    12481 gcttcaacac gctcgcgcgt gcgctgactc acgccgcttt cgttattcag cacccgggaa
    12541 accgtagatt tccccacgcc gcttaagcgc gcgatgtctt tgatggtcag ccgattttgc
    12601 atcctgttgt cccataacgt gttgtttaat tatttgagcc taactttacc cgtgcattca
    12661 gcaatgggta aagtctggtt tatcgttggt ttagttgcca gcaggtatta tatcgccata
    12721 aatgctacga atattattgg attctcctta ttatttgcgg cgcttttttc acttaccgga
    12781 ggttatatgg aacctgatcc cacgcctctc cctcgacgga gattaaaact tttccggtaa
    12841 gcccgtcttt tcacggcgtt accggatgcg taaggccgtg acgttttaat gtccctgctc
    12901 agctttatta ccttcaggta aggcttcgcc acgcctgaag acatttctgt actgtttcag
    12961 acagtgcgga gggactcctt atgtttaaag aaatttttac ccggttcatt cgccatttac
    13021 cttcccgtct ggttcatcgc gatccattgc ctggcgcgca gcagacagtg aatacggcgg
    13081 tcccgccgtc cttaagtgcg cattgcctga aaatggcggt gatgcccgaa gaagaattgt
    13141 ggaaaacgtt cgacacccat ccggaaggat taaatcaggc ggaagtggaa tctgcccgcg
    13201 aacaacatgg tgataataaa ttacccgccc aacaaccgtc gccgtggtgg gtacatttat
    13261 gggtctgcta tcgcaacccc tttaatattt tgctcaccat tcttggtgct atttcttacg
    13321 ccacggaaga tttatttgcc gcaggtgtta tcgcgctaat ggtcgctatt tctacgttgc
    13381 tgaactttat tcaggaagct cgttccacca aagcggcaga tgccctgaaa gcgatggtca
    13441 gtaataccgc gacggtactg cgagtaatta acgacaaagg cgaaaatggc tggctggaga
    13501 tcccgataga ccagctggtg cccggcgata ttataaaact ggcggcgggg gatatgatcc
    13561 cggcagattt acgtatcttg caggcgcggg atctgttcgt cgctcaggcg tcgttaaccg
    13621 gtgagtctct gcccgtggaa aaagccgcta ctactcgcca gccggagcac agcaatccgc
    13681 tggagtgcga caccctgtgt tttatgggca ccaccgtggt gagcggcacg gcacaagcaa
    13741 tggtgattgc tacaggagcc aacacctggt ttggtcaact ggcggggcgt gttagtgagc
    13801 aggaaagcga gccgaatgcc tttcagcaag ggatcagccg cgttagtatg ctgctgattc
    13861 gctttatgct ggtgatggct ccggtggtgt tgcttatcaa cggttacacc aaaggcgact
    13921 ggtgggaagc ggcactgttt gcgctttcgg tcgcggtagg cttaacgccg gaaatgttgc
    13981 cgatgattgt tacctcgacg ctggcgcgcg gcgcagtaaa gctgtcgaaa cagaaagtga
    14041 tcgtcaaaca tctggatgcc attcagaact ttggcgcaat ggatattctg tgcactgata
    14101 aaaccggcac cctgacgcag gacaaaattg tgctggagaa tcataccgat atctccggta
    14161 aaaccagcga acgcgtgctg catagcgcgt ggttgaacag tcattaccag accggactta
    14221 aaaatctgct tgataccgcg gtgctcgaag gtacggatga agagtcagcg cgttcgctgg
    14281 ccagccgttg gcaaaaaatt gatgagattc cgtttgattt cgagcgtcgt cggatgtcgg
    14341 tggtagtggc agaaaatacc gaacaccatc agctggtttg caaaggtgca ttgcaggaaa
    14401 tcctcaatgt gtgttcgcag gtgcgtcaca atggcgagat tgtaccgctc gatgacacca
    14461 tgctgcgtaa gattaagcgg gttactgata cgctgaatcg tcaggggctg cgcgtggttg
    14521 cggtggcgac gaaatacctg ccagcgcgtg aaggagatta ccagcgggcg gatgaatccg
    14581 acctgatcct cgaaggatat attgctttcc ttgatccgcc aaaagagaca accgctccgg
    14641 cactgaaggc attaaaagcg agtggaatta ccgtaaaaat ccttactggc gacagtgagt
    14701 tagttgcagc gaaagtgtgc catgaagtgg ggctggatgc gggagaggtg gtcattggta
    14761 gtgatattga aacgctatct gacgatgaac tggcaaatct cgcacagcgc accacgctgt
    14821 ttgcccgcct gacgccgatg cataaagaac gcattgtgac tttactgaag cgcgaagggc
    14881 atgtggttgg ctttatgggc gatggtatta atgatgcgcc cgccttacgc gctgcggata
    14941 tcggcatttc tgtggacggc gcggtagata ttgcccgtga agcggctgat atcatcctgc
    15001 tggaaaaaag cctgatggtg ctggaagagg gggttattga gggacgtcgc actttcgcca
    15061 acatgctgaa atacatcaaa ttgacggcga gttctaactt cggtaatgtg ttcagcgtgc
    15121 tggtggcgag tgctttcttg cccttcctgc cgatgttgcc gttacacttg cttattcaga
    15181 acctgctgta cgatgtgtca caggtggcga tcccgtttga taacgtcgac gacgagcaaa
    15241 ttcaaaagcc gcagcgttgg aatccggcgg atctggggcg ctttatggtc ttcttcggac
    15301 cgatcagctc gattttcgat attctgacgt tttgcttgat gtggtgggta ttccatgcca
    15361 acacgccgga aacgcaaacg ctgttccggt cgggctggtt tgtggtggga ttactgtcgc
    15421 aaacgctgat tgtgcatatg atccgtaccc gccgtgtgcc gtttattcag agctgtgcat
    15481 cgtggccgtt aatgatcatg accgtgatcg tgatgattgt cgggatcgca ttgccgtttt
    15541 caccgctggc cagttatctg cagctgcagg cgctgccgtt aagctatttc ccgtggctgg
    15601 ttgcgattct ggcagggtat atgacgttaa cccagttggt gaaagggttc tatagccgtc
    15661 gttacggctg gcagtaaaga ataaactggg catggtggcc cggattcaga ttgcagacaa
    15721 agtgagcttt gttcatgccg gatgcggcgt aaacgcccaa tccggcctac aaattcaata
    15781 aattgcagga atcacgtagg cctgataagc gtagcgcatc gggcgatttt gcttttgtca
    15841 tcagacttaa tccgggcatg atagcccgga tttccatcaa gattagcgac gaacagcgat
    15901 tgcttcgatc tcaatcttca cgtctttcgg cagacgggca acttcaacgc aagaacgtgc
    15961 cgggaaagtg gcgttgtgtt cggtgaagaa ggcttcgtaa gtggcgttta cggttgcgaa
    16021 gtcgttcaga tctttcacga acacagtggt tttaacgatg tcgcccactt tcaggccagc
    16081 ggcttcgacg attgctttta cattatccag cgactgacgt gcctgtgcag cgacgtctgc
    16141 cggtacttca ccagttttcg gatttaccgg gatctgaccg gaggtgatga tcatattgcc
    16201 cagatcaacg ccctgtacgt aaggaccgat agctgccggt gcattttccg tcgcgatagt
    16261 tttgctcatg atttctcctt tattacagcg gtaaaaagtc tggctcatta tagggagcca
    16321 gacttttatt accaaccgca attaattggc cagcaccaca ttatgggaaa actctttttc
    16381 acagtatttg catttaagcg cgatgtcatt ggcgcgtttt cgcacggcaa agctggatga
    16441 aaccggttcg gcatggctga tacagttgct gttcgggcag accagcacat tgtcgatgcg
    16501 ctctggcaga cttgggcgcg atttacccac cacttcatag ttgtcgatac ggttaaccgt
    16561 ggcttgcggt gcatacaatg ccagttggtc tacttgctct tcactcaaaa aggtattttc
    16621 gattttgatc agatctttgc ggcccatctc gccagaaggc aaattcagac caatggtgat
    16681 gcgctgatct gtttcggtca acttgaacag actcaacagc ttaaaaccga tctgggcggg
    16741 gatatggtca attaccgtgc cgcgtttaat agcttcaacc tgcaatttat tatcgtgtgt
    16801 catctctatt tccccttaca gtaccagatc gcgattcaga accagtgcca gtaacgcctg
    16861 gcgagcgaaa atcccgttgc ctgcttgctg gaagtaccag gcgtgaggcg ttttatccac
    16921 atccgtcgca atctcatcaa cacgcggcag cggatgcagc actttcatat tggctttggc
    16981 gttgtggaga tcgctggcgc gaagaacaaa ctgcgctttc acgttggcgt actcggacgg
    17041 gtccagacgc tctttttgca cgcgggtcat gtacaggatg tctacttccg ccatcacttc
    17101 ttcaatagag ctgtgcagac tccaggcgat ccctttttca tcgagcatat ccagaatgta
    17161 ttgcggcatt gccagcgcat ccggcgcgat aaagtaaaaa cggttgccgt cgaacttcgc
    17221 tagcgcctgg gtcagggagt gaacggtgcg gccatatttc aggtcaccaa ccattgcgac
    17281 gtggagattg tccagacgcc cctgggtttc ctgaatggtg aataagtcca gcaaggtttg
    17341 cgtcggatgt tggttggagc catcaccggc attcagtacc ggtacattgc cagaaaattc
    17401 ggtggctagg cgtgccgcac cttcctgcgg atgacgcatc actatcgcat cgacgtaagt
    17461 gctgataacc gaaatggtat cggccagcgt ttcgcctttt ttacccagtg atgtattggc
    17521 gctgtcggag aagcccacca cgctggcacc caggcggtgc atggaagttt cgaaagagag
    17581 gcgggtacgg gtagaggctt cgaagaaaca gctggcaatg actttgtgct tcaacagctc
    17641 tggttgcggg tttgctttca gtttcgccgc tgtcgccagc accagattaa ggtcatcgcg
    17701 actaaggtcg tttatggaaa tgatatgttt ctgatatagc ggattagcca tcttttatct
    17761 cctgacacct aggcaaaaaa aagcccctcg attgaggggc tgggaatggg tgatcaacgg
    17821 gaagaaaaac ggcaggccag cgtctttttt cagacgcggt aagacaaaat gtcgaacaca
    17881 ctgaaccata catcctcccg gcaaattgtc cggcattata ctcatagcca gaagcggtgc
    17941 aagcatttga tgcaatattt tgtcagcgca aacggtttat ttgaattaaa agtcaaaata
    18001 tatgcatttt tattttctat gattctggag gggaagtgtc gttcggtatc aataattgca
    18061 gacatttcct gcttttttct ttttcatttt cacgcaatca gaaatgcatt ttattccatt
    18121 catccgactt atagggcgga gggtgaaaac gaacggctaa cactattgct tatagctcag
    18181 ggatacgcgc tatcacttta atttcaaaat caaagcctgc cagccaggta acacccaccg
    18241 ccgtccagtt tggataaggt ggggcgctaa atatttcatt tttcaccgtc atgatgtctt
    18301 cagattgttt ttccggatcg gtatgaaagc tcgtaacatc aatgatatcg tcaaaagtgc
    18361 atcccgcagc tgccagggtc gcatgcaaat tatcaaaggc cagtctgact tgttgctgaa
    18421 aatcgggttc tggtgttccg tcctctcgac ttcctacttg cccggaaaca aacagcaaat
    18481 cgccggaacg aatagccgca gaataacgat gctcagcata cagtgaatgt cggccagcag
    18541 ggaaaacagc ggttctttct accatttggt tatcctcaag atttacgaca tgaacagaag
    18601 atttatattt atcgggagcc gcttttaggg ggacgacgtt agtaaacaaa acccagacat
    18661 catggataat ggctgggctt aattgagcgt agtcggttat gcgccaaacg cgccatcaat
    18721 ggtatgcatc gcgccggtaa caaaactggc ttctggccct gctaaccatg cgaccatacc
    18781 agcgacctct tccggttgcc catgtctttt gatggccata aaactatgca acatatcgcg
    18841 cattggcccg ttggcgggat taacgtcggt atcaattggt cctggctgga cgacgttgat
    18901 ggtgatccct cgcggtccaa aatcacgggc cagcccgcgc gccatgcctt gcagggcaga
    18961 tttactggcg gcataagcag ccatgcctgc aacaggcata cgatcgccat tcacggagcc
    19021 gatgattaag atgcgcccgc cttcgggcat ctgccgggcg gcttcaacag aggcatgata
    19081 aggagcatga atattgattt tgaaaaggcg atcaatatcg tcggcattta attccagggc
    19141 atcgccaaag acgccaatac ctgcatttac caccaggata tccaatgcgc cgctcttacg
    19201 aacgacatca ataacagcgt ctctgtcagc actatctgtg aatactgctg tcgctccagt
    19261 ctcttgtgcc agacgttcag cggcatcttt cgaccccgca taggtgaatc gtacattggc
    19321 cccatcggtg acgaaacgac gtacgatagc ggcaccgatt ccacgactgc caccgaggat
    19381 gagaactgtc ttaccggtaa aagcgcccat aaggactcct tgatttatta tgtaacatgc
    19441 attacaaaac tgttttaact ttctgtcaac aggttttgta atggtcacta aaaaacaatc
    19501 tcgcgttcca ggtcgtccca gacgtttcgc tcctgagcag gcagtctctg cggcaaaagt
    19561 gctttttcac caaaaaggtt tcgatgcagt aagtgtctct gaagttactg attatcttgg
    19621 tattaacccc ccgagtctct acgcggcttt tggcagtaag gctgggttat ttagccgtgt
    19681 actcaatgaa tatgtcggta cggaagctat tccgcttgcc gatattcttc gtgatgatcg
    19741 tccggtaggc gagtgcctgg ttgaggtatt aaaagaagcg gcgcgcagat atagccaaaa
    19801 cggcggctgc gctggctgta tggttcttga aggtattcat agtcatgatc cacaagcgcg
    19861 tgatattgcc attcaatatt atcacgccgc agaaacgacc atttatgact atatcgccag
    19921 gcggcatcca caaagcgcac aatgtgtgac tgattttatg agtaccgtga tgtcagggct
    19981 ttctgcgaag gcacgggagg ggcactccat cgaacaactc tgtgcaacag ctgccctggc
    20041 aggggaagcg ataaaaactc ttctcaagga gtgatgctgg ccttgatccg aaaggcggga
    20101 acgcgcctgc cgataagttg tgataagaca ataattcacg cgttaaggct ggcggaattg
    20161 atcatctttt cgtataacga tagaaatgaa acattgtttt aattaaggag tggaaaagat
    20221 gatcatcgga aatattcata atcttcagcc gtggctaccc caggagttac gccaggcgat
    20281 tgagcatatc aaagcacacg ttacggcaga aacgccaaag ggcaagcatg atatcgaagg
    20341 caatcgtctg ttttatctta tctcggaaga tatgaccgag ccgtacgaag ctcgccgtgc
    20401 ggagtaccat gcccgctatc ttgacattca gattgtgtta aaaggtcagg aaggcatgac
    20461 cttcagcacg caacctgcag gcgcgccgga taccgactgg ttagctgata aagacatcgc
    20521 atttttgccg gaaggcgttg atgagaaaac agttatccta aatgaaggtg attttgttgt
    20581 gttttatccg ggggaagtgc ataaaccgct gtgcgcagtg ggcgcgccag cccaggttcg
    20641 caaagcagta gtgaagatgc tgatggcgtg atgacttttc gccgtaaata actccaggtt
    20701 tacggcgagt ttgtgaaaag agcgtttttt gatatttttt tgtgagtaaa atttgtaatg
    20761 cttagacgtt cttattaact caaggagttc gtcatgggca aaatatcaga tttgaattat
    20821 tctcaacaca ttacattagc cgacaatttt aaacaaaaaa atgaagcctt agatacctgg
    20881 tatgtgggaa tgaatgattt tgcccgaatt gccggggggc agaatagcag aagcaatatt
    20941 ctttctccta gagcattttt ggagtttttg gcaaagatat ttaccctggg ttatgtggat
    21001 tttagcaaac gctccaacga agcgggtaga aatatgatgg ctcatattga gttctcatct
    21061 tattctaaag atactgatgg caatgaaaaa atgaagtttt acatgaataa tcctgaaggg
    21121 gagcgagcgg atttatctaa ggtgaagata gaaattacac ttgcctcagc ttctactaaa
    21181 gggattcgtg aaggacatac agtcattata tttaaacaat ctgatggttc gactaatcgt
    21241 tatgaaggga agtcttttga aagaaaagat gatagttcat tacacctgat tactaacaag
    21301 gttctggcgt gttaccaacg cgaagctaac aagaaaatag cgcgtctatt aaataatcat
    21361 cagaagttaa ataatataca ggagttaaat gattcgcagg agttaaataa ttcacagaag
    21421 ttaaataatt cgcaggagtt aaataactcg caggtgagtt gtaaaggttc agttgattct
    21481 acgattacgg atttattaga aaaagcattg aa
//
LOCUS       DAZPCS010000053        21510 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZPCS010000053 DAZPCS010000000
VERSION     DAZPCS010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21510
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.053"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(42..2111)
                     /gene="glyS"
                     /locus_tag="ACHUQ7_001991"
     CDS             complement(42..2111)
                     /gene="glyS"
                     /locus_tag="ACHUQ7_001991"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFR1135414.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2121..3032)
                     /gene="glyQ"
                     /locus_tag="ACHUQ7_001992"
     CDS             complement(2121..3032)
                     /gene="glyQ"
                     /locus_tag="ACHUQ7_001992"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFR1135415.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3127..3426)
                     /locus_tag="ACHUQ7_001993"
     CDS             complement(3127..3426)
                     /locus_tag="ACHUQ7_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFR1135416.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVRVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3601..4596
                     /gene="wecH"
                     /locus_tag="ACHUQ7_001994"
     CDS             3601..4596
                     /gene="wecH"
                     /locus_tag="ACHUQ7_001994"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFR1135417.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4638..5075)
                     /gene="yiaA"
                     /locus_tag="ACHUQ7_001995"
     CDS             complement(4638..5075)
                     /gene="yiaA"
                     /locus_tag="ACHUQ7_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HFR1135418.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5121..5462)
                     /gene="yiaB"
                     /locus_tag="ACHUQ7_001996"
     CDS             complement(5121..5462)
                     /gene="yiaB"
                     /locus_tag="ACHUQ7_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFR1135419.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5631..7085)
                     /gene="xylB"
                     /locus_tag="ACHUQ7_001997"
     CDS             complement(5631..7085)
                     /gene="xylB"
                     /locus_tag="ACHUQ7_001997"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFR1135420.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7157..8479)
                     /gene="xylA"
                     /locus_tag="ACHUQ7_001998"
                     /pseudo
     CDS             complement(7157..8479)
                     /gene="xylA"
                     /locus_tag="ACHUQ7_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            8845..9837
                     /gene="xylF"
                     /locus_tag="ACHUQ7_001999"
     CDS             8845..9837
                     /gene="xylF"
                     /locus_tag="ACHUQ7_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR1135421.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKKA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            9915..11456
                     /gene="xylG"
                     /locus_tag="ACHUQ7_002000"
     CDS             9915..11456
                     /gene="xylG"
                     /locus_tag="ACHUQ7_002000"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HFR1135422.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVERELTAIYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     ITQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11434..12615
                     /gene="xylH"
                     /locus_tag="ACHUQ7_002001"
     CDS             11434..12615
                     /gene="xylH"
                     /locus_tag="ACHUQ7_002001"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HFR1135423.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12693..13871
                     /gene="xylR"
                     /locus_tag="ACHUQ7_002002"
                     /pseudo
     CDS             12693..13871
                     /gene="xylR"
                     /locus_tag="ACHUQ7_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription.; internal stop;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
     gene            complement(13979..14803)
                     /locus_tag="ACHUQ7_002003"
     CDS             complement(13979..14803)
                     /locus_tag="ACHUQ7_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HFR1135424.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWAPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15123..17153
                     /gene="malS"
                     /locus_tag="ACHUQ7_002004"
     CDS             15123..17153
                     /gene="malS"
                     /locus_tag="ACHUQ7_002004"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFR1135425.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTSYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASSALREWKKTNPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            17331..18584
                     /gene="avtA"
                     /locus_tag="ACHUQ7_002005"
     CDS             17331..18584
                     /gene="avtA"
                     /locus_tag="ACHUQ7_002005"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFR1135426.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEGRPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18735..19208)
                     /locus_tag="ACHUQ7_002006"
     CDS             complement(18735..19208)
                     /locus_tag="ACHUQ7_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFR1135427.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(19310..20158)
                     /gene="yiaJ"
                     /locus_tag="ACHUQ7_002007"
     CDS             complement(19310..20158)
                     /gene="yiaJ"
                     /locus_tag="ACHUQ7_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HFR1135428.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            20358..21356
                     /gene="yiaK"
                     /locus_tag="ACHUQ7_002008"
     CDS             20358..21356
                     /gene="yiaK"
                     /locus_tag="ACHUQ7_002008"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HFR1135429.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21368..>21510
                     /locus_tag="ACHUQ7_002009"
     CDS             21368..>21510
                     /locus_tag="ACHUQ7_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFR1135430.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATNFNVLEPGVVEIDGKN
                     IYA"
BASE COUNT         5408 a         5489 c         5278 g         5335 t
ORIGIN      
        1 aaaaagcctg ccagatggca ggctttttaa taacggcgtt attactgcaa cagcgaaata
       61 tccgcaacgc gcaggaacag ttcgcgcagt ttctccagca tggtcagacg gttgagacgc
      121 aattctttgt catcaaccat gaccatcact ttatcgaaga aagcatcaac cggctcacgc
      181 agctctgcca gttcgaccag cgcatcctgg taacgacctt ccgcaaagta cggctccagc
      241 ttgtcacgta gcaccacaac ctgcatcgcc agtttaattt cttccggctc tttcagggta
      301 gaggcattca cgcgatcgct cagcacttcg tcagatttcg ccaggatgtt agatacacgt
      361 ttgttcgccg ccgccagtgc tgcagctgct tccagggtac ggaagtgcga taccgctttc
      421 atgcgggcat cgaaatcagc cggacgagtc ggacgacgcg ccagtaccgc ctggatggtg
      481 tcaacggtat aaccttcgtc ctgataccag gcgcggaagc gaccgagcat aaagtcgata
      541 acatcatcaa ctacgttggc attagtcagc ttatcgccat acagacgcac cgcttcttcg
      601 gtcagcgttt gcagatcaag gttgaggttc ttctcaacga taattcgcag cacgccaagt
      661 gcggcacgac gcagcgcaaa cgggtctttg tcgcctttcg gatgctgacc gataccgaag
      721 atacccgcca gagtatccat cttatcagca atcgccagcg cacaagctac cgggttggac
      781 ggcaggtcat caccggcaaa gcgcggctga tactgctcat tcagcgcgac ggctacgtct
      841 tccgcttcgc catcgtgacg cgcgtagtgc atccccataa cgccttgggt gtcggtgaac
      901 tcgaagacca tgttggtcat caggtcacac ttagacagca ggcccgcacg ggttgcgtgg
      961 ttaacgtcag cgccaatctg ttcggcgatc cagccagcca gcgcctggat gcggtcagtc
     1021 ttgtcacgca gcgtacccag ctgttgctgg aacagcacgg tttgcaggcg cggcaggtta
     1081 tcttccagac gttttttgcg gtcggtgttg aagaagaact cggcatccgc cagacgtgga
     1141 cgaacgactt tctcgttacc agagataatt tgctgcggat ctttcgattc gatgttggca
     1201 acgaagataa agttcggcag cagtttgccg tcgttcgcat acaccgggaa gtacttctgg
     1261 tcacctttca tggtgtaaac cagggcttca gccggcaccg cgaggaattt ctcttcgaat
     1321 tttgcggtca gaacgaccgg ccactccacc agcgaagcca cttcttccag caggctttcg
     1381 cttaagtcag cgttaccgcc aatcttacgc gctgcttctt cggcatcggc tttaatcttc
     1441 gctttacgtt cttcgtaatc ggcgatgact ttcccgcgct cacgcagaat ttccggatac
     1501 tgatcggcgt tgtcgatggt gaattccggt tcgcccataa agcggtggcc gcgaatcacg
     1561 cgatcggact gaatgcccag aatggttgcc ggaatgactt tgtcgcccag cagcagggtc
     1621 acggtgtgca ccggacgcac gaagtgcacg tcgcttgcac cccagcgcat cagtttcggg
     1681 atcggcagtt tcgccagcga agtcgcaacc atattcggca gcagtgcttc ggtgctttcg
     1741 cccttcacat gggcgcgata cagcagccat tcgcctttat cggtagtcag acgctcagcc
     1801 tggtcaacgg taataccaca accacgcgcc caaccttctg ccgctttgct cggttttcct
     1861 tcagcgtcga acgcctgggc aatcgccggg ccgcgttttt cgatttcacg atctggttgt
     1921 gcttccgcca ggttagctac tttcagcgcc agacgacgcg gagcagcaaa ccattgaacg
     1981 gtgccgtgtg cgaggcctgc gttatccagc tccgcagtaa agttcgcagc aaaggactct
     2041 gccaggctgc gcagtgcttt tggtggcagc tcttcagtgc cgatttccac cagaaaagtt
     2101 ttctcagaca tagccgcctc ttacttatct ttgttgcaca tcgggaagcc gagggcttca
     2161 cgggaagcgt aatatgcttc tgccactgct ttggtcaggg tgcgaatgcg cagaatgtag
     2221 cgctgacgct cggtgacgga gatggcttta cgcgcatcca gcaggttgaa gctgtgggcg
     2281 gctttcagaa tacgctcgta ggctggcagc ggcagcggat tttccagcgc cagcagctgc
     2341 tgcgcttctt tctcgtactg ctcaaagcag gtgaacagga agtccacatc cgcgtattcg
     2401 aagttgtaag tggactgctc cacttcgttc tgatggaaca cgtcgccgta ggtggtttta
     2461 cccagcgggc cgtcgctcca gactaggtcg taaacgctgt ctacgccctg aatgtacatg
     2521 gccagacgtt ccagaccgta ggtgatctcg ccggtaaccg gtttacactc cagaccacca
     2581 acctgctgga agtaagtgaa ctgcgtcact tccatgccgt ttagccacac ttcccagccc
     2641 agtccccagg cccccagagt cgggttttcc cagttatctt ccacgaaacg gatgtcgtga
     2701 atagtcgggt ccatgcccag ctctttcaga gaaccgaggt acagctcctg aatattgtcc
     2761 ggcgatggct taatgaccac ctggaactga tagtagtgct gtaaacggtt ggggttttcg
     2821 ccgtagcgac catcggtcgg gcgacgagaa ggctgaacat aagcagccgc catcggttct
     2881 ggccccagcg cgcgcagaca ggtcattggg tgagaggttc ccgcgccgac ttccatgtcc
     2941 aatggttgaa caatggtgca gccctggcga gcccagtaat cctgtaaggt caggatcaag
     3001 ccctggaagg tcctggtatc aaacttttgc atattatttc gtgctggata cgtgtggatt
     3061 taaaggaagg gatcagtata cccgctggat ggaagatata cagtacgaaa cgggaaaaag
     3121 cagggcttaa cgcatggaaa gatgcaaaaa atggccatct gcgtcaaaag aacagacaaa
     3181 ctgctcttta cggaaggtat aaccgcgcat ttcatagctt ccctggaact gttcgaaggc
     3241 ggtgacgtcg attttttgct cgccggtgtt ataacgtttt gccgcctgat ccttgcataa
     3301 cgcttccata ttcaacgaac gcagaggatc aacccttacc cgttgtgctt tctgcacggg
     3361 tgacggtgta gaacaaccca ctagcaccat aagcgccatt gccggaaaga aagcgttcat
     3421 catcattaaa ttaccgtctg gtttgcaggt gagtcttatt atttatatcg gtatagtggc
     3481 tgatatctta cgaatttcct taagcctcta taggctgtag tgataaaact cagattattc
     3541 cagtgcgcca accaaatgat ttatcggtca gacttttttt aaccagatac agagacacag
     3601 atgcagccca aaatttactg gattgataac ctgcgaggga tagcgtgttt aatggtggtg
     3661 atgattcaca ccactacctg gtatgtgacc aatgctcata gtgttagccc cgtcacatgg
     3721 gatatcgcca atgttctgaa ttctgcctct cgtgtcagcg tgccgctatt tttcatgatt
     3781 tccggctatc tcttttttgg cgaacgcagc gcccagccgc gccatttctt gcgtatcggc
     3841 ttatgtctga ttttttatag cgcaatcgca ctgctctaca ttgcgctgtt tacctccatc
     3901 aatatggagt tagcgctgaa aaacctgctg caaaagccag tgttttacca cttgtggttt
     3961 ttcttcgcga ttgcggtgat ttatctggtt tcaccgctga ttcaggtgaa gaacgtcggc
     4021 ggaaaaatgt tgctggtgct aatggtggtg attggcatta tcgctaaccc aaacacagtg
     4081 ccgcagaaaa ttgacggttt tgaatggctg ccaattaact tatatatcaa tggcgatact
     4141 ttttactaca ttctgtatgg catgttgggc cgcgctatag ggatgatgga cacacagcat
     4201 aaagcactgt cgtgggtgag cgccgcgctg tttgcgacgg gggtttttat tatctctcgc
     4261 gggacattat atgaattgca gtggcgcgga aattttgccg atacctggta tctttactgt
     4321 gggccgatgg tttttatctg cgcaatcgcg ctattgactc tggttaaaaa cacgctggat
     4381 acgcgtacca ttcgcggact tggcttaatc tcccgccatt cattgggtat atacggattc
     4441 cacgccttga ttatccatgc gctgcgcacc cggggaattg agcttaaaaa ttggccaata
     4501 ctggatatta tttggatctt ttgcgcgacg ttggcagcga gtttgttact ttctatgctg
     4561 gtacaacgaa tcgacagaaa cagattagtg agttaagtaa aagcccgttc acattggact
     4621 gaccgggctt acgtgaatta ttcgctgtat tcttcctggg taacctgtgt ttcttttggc
     4681 gggtttattc cggcatcacg aatattcttc tgcaccgcta ctgcaccaaa caagcttaag
     4741 aagaaagcca gtccataaaa acctttttca ctgagtaata atgtcgcatt ccacagacct
     4801 accatcagta gagcaacaga gataataaag acagtcaggc aggtcatata ataaatggaa
     4861 gtggtcggta tgccttcata cttgtcccga acggtctttt gataagacgc agcagaaaac
     4921 agtcctaaca ccagtacggc aaaataatac cctttttcat ttaactgcat ctctgcattc
     4981 catagcccta acaggtaggt aacgatacca ccaacgagag ctatccatga cacaatacta
     5041 aaggccggtg aataggttga tattttgttg tccatcacag tattcctttc attcctgaat
     5101 gtgtaagagc tttatgttgc tcataccgag cgaaacaaac gcatttgacc aaacaagcag
     5161 acaaaaaagg cgatggggta gatcaccatt tcataagcat tgataggtgc atttaaaaca
     5221 ccgataaaca agagaccgat agtgattaac gcaactaact ggcagatatg ggtaaaaaaa
     5281 ttatccagtt gcccgagttt ttctgcgcga agatatgcat agttaccaaa agttgctgtc
     5341 attaacacgc caagaaaata gccttttcca cttagcaatg ggcatgagat ccatagccca
     5401 accagataaa ccagcgcccc gacaacaaat aatagttttg ccacagtttt tgaggttttc
     5461 ataacgatct ccatatctac cagcgataca ttacgagtaa ccaacgaaag acaaaactga
     5521 aaaatgccat taacaaatga ttttcagaat aaattcatac taaatattaa ttaattagtg
     5581 agatatatag atgtgaatta tcccccaccc ggtcaggcag gggataacgt ttacgccatt
     5641 aatggcagaa gttgctgata gaggcgacgg aacgtttcac gtcgtggctg ataagcggca
     5701 taacgctgcg catctggtag atgcgactgt tctaacggta gctgcggcaa caactcaatg
     5761 agcgatttct ctggattcgc cgcaatctgc gccagccttg ctgcgcccag tgctggcccc
     5821 acatcccccc ccgtacggta atcgagctgc tgaccgctga tatccgccag catctgacgc
     5881 cagtactcac tacgcgcccc gccaccaatc aacgtaacac tttgcggttt aatgccgcag
     5941 gcatgcacga catccatgcc atctgccagc gcataaccca cgccttccag cactgctcgc
     6001 gccagttcat tggggccatg ttgatgagtc aaaccaaaga aaaccccctt cgcctgggga
     6061 ttattgtgtg gtgtacgctc gccggaaaga taaggcagaa accaaactgg ctcggcactt
     6121 tcatcagcct gttgagctgc agcgattaaa gctgggacat tgcacaggcc ggttaatttc
     6181 gcggcccaat ccagacacga tgctgcactc agcattacag acattaaatg ccaacgctgc
     6241 ggtagcgcat ggcaaaagct gtgtacagcg ctttctggct tgcttaagaa cccttcgctg
     6301 acagcaaaat agacccccga cgtccccagc gataacattg cctgattagc atcaaccatt
     6361 cccacaccaa ctgcaccagc tgcattgtcg ccaccgcctg cgacaactgg caccgtcgcc
     6421 ataccccacg ctttcgcaac ttcaggtaac aaagcaccag taatttcgct gccttcgtat
     6481 aatgcgggca tctggtcacg agataagtcg caagcctgca gcatgacgtc actccagtca
     6541 cgctttgcga catccagcca catggtgcca gctgcgtcag acatatcgct ggcaaactcc
     6601 cccgtcatac gcagacgcaa gtaatctttc ggtaataata ctttgtcgat ttgacggaat
     6661 atctccggct catgccgctg aacccatagc aatttaggcg cagtaaatcc gggcatcatc
     6721 aggttgccgg taatcactcg tgattgcgga actctcgctt ccagcaaagt gcactcttgc
     6781 gcacagcgcc cgtcgttcca caaaatggca gggcgcaata cccgttgttg agcatccagt
     6841 aaggttgctc catgcatctg gccggcaata cccaatgctt taacgtcctg cagagaatgc
     6901 tgatcgccca gagctttcat tgcgcgatca gttgcctgcc accactgttc cgggtcttgt
     6961 tccgaccaaa gtggatgcgg gcgcgaaacg gtcagctttt ccgtttgcga agcaaccacc
     7021 tcaccctgct cgttgagcaa aataactttt acgcccgagg tgccaagatc tatcccgata
     7081 tacatatcga tcgttcctta aaaaaatgcc cggtatcgct accgataacc gggccaacgg
     7141 actgcacagt tagccgttat ttgtcgaaca gataatgatt taccagattt tccagttgct
     7201 cctggcgacc actctgatgc accggagaca aattatgttc ctgagcatat ttggctaaat
     7261 ctgccagtga catttggcct ttcaggattt gctggcccaa ttcgctattc cagccggaat
     7321 aacgctgcgc gatgcgttta tccagctcgc catcttcaat catgcgcgct gcaattttca
     7381 gcgccagtgc catcgtatcc atcgcgccga tatgaccgta aaacagatca tatttatcag
     7441 tactttgacg acgtactttg gcatcgaagt tcagaccacc ggtggtgaaa ccgcctgctt
     7501 tgagaatttc atacatcacc agcgcattct cttccacact gttcgggaac tggtcggtgt
     7561 cccagcccag ttgcgcatcg ccacggttgg cgtcgacaga accgaacagg ccaagcgcaa
     7621 tggcggtggc tatttcatga tggaaagagt gacctgccag cgtcgcgtgg ttagcttcaa
     7681 tgttcagttt aatctctttt tccagaccaa actgtttcag gaagccatag accgtcgcgg
     7741 catcgtaatc atattgatgt ttagtcggtt cttgcggttt cggttcgata agcaacgtgc
     7801 cctagaagcc gattttatgt ttatgctcaa ccaccatctg cataaagcgg cccagttgtt
     7861 cacgctcctg acgcaagtcg gtatttaaca gcgtttcgta accttcacga ccaccccaca
     7921 ggacatagtt ttcaccgccc aatttatggg ttgcttccat cgctgtaaca acttgcgttg
     7981 ccgcccagct gaagacttca ggatctgggt tcgtcgccgc acccgcgcca tagcgagggt
     8041 ttgtaaagca gttagcggtt ccccacagca gcttcacgct gctctcttct tgcttggctg
     8101 ccaggacatc aaccatttgc gcaaaattat tgatgtactc ttttaacgac gcgccctcag
     8161 gggaaacatc cacatcgtgg aagcaataaa atggcacatg taacttgtgg aaaaactcaa
     8221 atgcgacatc tgctttacgc ttcgccaacg ccagtgcctc accaggctgc tgccacggac
     8281 gattaaacgc ccccacacca aacatgtccg ccccgttcca gcagaaggtg tgccagtagc
     8341 aggcggcaaa acgcaagtgc tcttccatac gcttacccaa caccagttcg tcgggattgt
     8401 agtgacggaa tgctaacggg tttgaggatt ttgagccttc ataacgaacg cgatcgagct
     8461 ggtcaaaata ggcttgcata ttgaactcca taatcaggta atgccgcggg tgatggatga
     8521 tgtcgtaata ttgggcactc cctttcaatt gctcaattat gttatttcac actgctattg
     8581 agataattca caagtgtgcg ctcgctcgca aaataaaatg ggatgatgaa aatgggtaat
     8641 tcctcgaaga taaaaatgca ataagtacaa ttgcgcaaca aaagtaagat ctcggtcata
     8701 aatcaagaaa taaaccaaaa atcgtaatcg aaagataaaa atctgtaatt gttttcccct
     8761 gtttagttgc taaaaattgg ttacgtttat cgcggtgatt gttacttatt aaagctgtcc
     8821 tctaactaca gaaggcccta caccatgaaa ataaagaaca ttctactcac cctttgcacc
     8881 tcacttctgc ttaccaacgt tgcggcacac gccaaagaag tcaaaatagg tatggcgatt
     8941 gatgatctcc gtcttgaacg ctggcaaaaa gatcgagata tttttgtgaa aaaggcagaa
     9001 tctctcggtg cgaaagtatt tgtacagtct gcaaatggca atgaagaaac acaaatgtcg
     9061 cagattgaaa acatgattaa ccggggtgtc gatgttcttg tcattattcc gtataacggt
     9121 caggtattaa gtaacgttgt aaaaaaagcc aaacaagaag gtattaaagt attagcttac
     9181 gaccgtatga ttaacgatgc ggatatcgat ttttatattt ctttcgataa cgaaaaagtc
     9241 ggtgaactgc aggcaaaagc cttggtcgat attgttccgc aaggtaatta cttcctgatg
     9301 ggcggctcgc cggtagataa caacgccaag ctgttccgcg ccggacaaat gaaagtgtta
     9361 aaaccttacg ttgattctgg aaaaattaaa gtcgttggtg accaatgggt tgatggctgg
     9421 ttaccggaaa acgcattgaa aattatggaa aacgcgctaa ccgccaataa taacaaaatt
     9481 gatgctgtag ttgcctcaaa cgatgccacc gcaggtgggg caattcaggc attaagcgcg
     9541 caaggtttat cagggaaagt agcaatttcc ggccaggatg cggatctcgc aggtattaaa
     9601 cgtattgctg ccggtacgca aactatgacg gtgtataaac ctattacatt gctggcaaat
     9661 actgccgcag aaattgccgt tgaattgggc aatggtcagg agccaaaagc agatacctca
     9721 ctgaataatg gcctgaaaga tgtcccctcc cgcctcctga caccgatcga tgtgaataaa
     9781 aacaacatca aagatacggt aattaaagac ggattccaca aagagagcga gctgtaagcg
     9841 ttacgccccg gcgcggagcg ggggcgtgat ttctctccat gccgcgtgaa tgaattggct
     9901 taggtggagt cgttatgcct tatctacttg aaatgaagaa cattaccaaa accttcggca
     9961 gtgtgaaggc gattgataac gtcagcttgc ggttgaatgc tggcgaaatc gtctcactct
    10021 gtggggaaaa tgggtctggt aaatcaacgc tgatgaaagt gctgtgtggt atttatcccc
    10081 atggctccta cgaaggcgaa attatttttg cgggagaaga gattcaggcg agtcacatcc
    10141 gcgataccga acgcaaaggt atcgccatca ttcaccagga attggccctg gtgaaagaat
    10201 tgaccgtgct ggaaaatatc ttcctgggta acgaaataac ccacaatggc attatggatt
    10261 atgacctgat gacgctacgc tatcagaagc tgctcgcaca ggtcagttta tccatttcac
    10321 ctgatacccg cgttggcgat ttagggcttg ggcaacaaca actggttgaa attgccaagg
    10381 cacttaataa acaggtgcgc ttgttaattc tcgatgaacc gacagcctca ttaactgagc
    10441 aggaaacgtc ggttttactg gatattattc gcgatctaca acagcacggt atcgcctgta
    10501 tttatatttc gcacaaactc aacgaagtca aagcgatttc cgatacgatt tgcgttattc
    10561 gcgacggaca gcacattggt acgcgtgatg ctgccggaat gagtgaagac gatattatca
    10621 ccatgatggt cgagcgagag ttaaccgcga tttaccctaa tgaaccacat accaccggag
    10681 atgaaatatt acgtattgaa catctgacgg catggcatcc ggtcaatcgt catattaaac
    10741 gagttaatga tgtctcgttt tccctgaaac gtggcgaaat actgggtatt gccggactcg
    10801 ttggtgccgg acgtaccgag accattcagt gcctgtttgg cgtgtggccc ggacaatggg
    10861 aaggaaaaat ttatattgat ggcaaacagg tagatattcg taactgtcag caagccatca
    10921 cccaggggat tgcgatggta cccgaagaca gaaagcgcga cggcatcgtt ccggtaatgg
    10981 cggttggtaa aaatattacc ctcgccgcac tcaataaatt taccggtggc attagccagc
    11041 ttgatgacgc ggcagagcaa aaatgtattc tggaatcaat tcagcaactc aaagttaaaa
    11101 cgtcgtcccc cgaccttgct attggacgtt tgagcggcgg caatcagcaa aaagcgatcc
    11161 tcgcccgctg tctgttactt aacccgcgca tcctcattct tgatgaacct accaggggta
    11221 ttgatattgg cgcgaaatat gagatctaca aattaattaa ccaactcgtc cagcagggta
    11281 ttgccgttat tgtcatctct tccgaattac ctgaagtgct cggccttagc gatcgtgtac
    11341 tggtgatgca tgaagggaaa ctaaaagcca acctgataaa tcataacctg actcaggagc
    11401 aggtgatgga agccgcattg aggagcgaac atcatgtcga aaagcaatcc gtctgaagtg
    11461 aaattggccg taccgacatc cggtggcttt tctgggctga aatcactgaa tttgcaggtc
    11521 ttcgtgatga ttgcagctat catcgcaatc atgctgttct ttacctggac caccgatggt
    11581 gcctacttaa gcgcccgtaa cgtctccaac ctgttacgcc agaccgcgat taccggcatc
    11641 ctcgcggtag gaatggtgtt cgtcataatt tctgctgaaa tcgacctttc cgtcggctca
    11701 atgatggggc tattaggtgg cgtcgcggcg atttgtgacg tctggttagg ctggcctttg
    11761 ccacttacca tcattgtgac gttggttctg ggactgcttc tcggtgcctg gaacggatgg
    11821 tgggtcgcgt accgcaaagt cccttcattt attgtcaccc tcgcgggcat gttggcattt
    11881 cgcggcatac tcattggcat caccaacggc acgactgtat cccccaccag cgccgcgatg
    11941 tcacaaattg ggcaaagcta tctccccgcc agtaccggct tcatcattgg cgcgcttggc
    12001 ttaatggctt ttgttggttg gcaatggcgc ggaagaatgc gccgtcaggc tttgggttta
    12061 cagtctccgg cctctaccgc agtagtcggt cgccaagctt taaccgctat catcgtatta
    12121 ggcgcaatct ggctgttgaa tgattaccgt ggcgttccca ctcctgttct gctgctgacg
    12181 ttgctgttac tcggcggaat gtttatggca acgcggacgg catttggacg acgcatttat
    12241 gccatcggcg gcaatctgga agcagcacgt ctctccggga ttaacgttga acgcaccaaa
    12301 cttgccgtgt tcgcgattaa cggattaatg gtagccatcg ccggattaat ccttagttct
    12361 cgacttggcg ctggttcacc ttctgcggga aatatcgccg aactggacgc aattgcagca
    12421 tgcgtgattg gcggcaccag cctggctggc ggtgtgggaa gcgttgccgg agcagtaatg
    12481 ggggcattta tcatggcttc actggataac ggcatgagta tgatggatgt accgaccttc
    12541 tggcagtata tcgttaaagg tgcgattctg ttgctggcag tatggatgga ctccgcaacc
    12601 aaacgccgtt cttgattttg ataaaaattt tctcaaagcc ggttacgtat taccggtttt
    12661 gagtttttgc atgattcagc aggaaaagaa ccatgtttac taaacgtcac cgcatcacat
    12721 tactgttcaa tgccaataaa gcctatgacc ggcaggtagt agaaggcgta ggggaatatt
    12781 tacaggcatc acaatcagaa tgggatattt tcattgaaga agatttccgc gcccgcattg
    12841 ataaaatcaa ggactggtta ggagatgtcg tcattgccga cttcgacgac aaacagatcg
    12901 agcaagcgct ggctgatgtc gacgtcccca ttgttggggt tggcggctcg tatcaccttg
    12961 cagaaagtta cccacccgtt cattacattg ccaccgataa ctatgcgctg gttgaaagcg
    13021 catttttgca tttaaaagag aaaggcgtta accgctttgc tttttatggt cttccggaat
    13081 caagcggcaa acgttgggcc actgaacgcg aatatgcatt tcgtcagctt gtcgccgaag
    13141 aaaagtatcg cggagtggtt tatcaggggt tagaaaccgc gccagagaac tggcaacacg
    13201 cgcaaaatcg gctggcagac tgactacaaa cgctgccacc gcaaaccggg attattgccg
    13261 ttactgacgc ccgagcgcgg catattctgc aagtatgtga acatctacat attcccgtac
    13321 cggaaaaatt atgcgtgatt ggcatcgata acgaagaact gacccgctat ctgtcgcgtg
    13381 tcgccctttc ttcggtcgct cagggcgcgc ggcaaatggg ctatcaggcg gcaaaactgt
    13441 tgcatcgatt attagataaa gaagaaatgc cgctacagcg aattttggtc ccaccagttc
    13501 gcgtcattga acggcgctca acagattatc gctcgctgac cgatcccgcc gttattcagg
    13561 ccatgcatta cattcgtaat cacgcctgta aagggattaa agtggatcag gtactggatg
    13621 cggtcgggat ctcgcgctcc aatcttgaga agcgttttaa agaagaggtg ggtgaaacca
    13681 tccatgcaat gattcatgcc gagaagctgg agaaagcgcg cagtctgctg atttcaacca
    13741 ccttgtcgat caatgagata tcgcaaatgt gcggttatcc atcgctgcaa tatttctact
    13801 ctgtttttaa aaaagcatat gacacaacgc caaaagagta tcgcgatgta aatagcgagg
    13861 tcatgttgta gcgatgatga gaattgtcgg cgtcacatca gggaactcag cacagccacc
    13921 tgatgcgagc aaaagcctta tccgaccgac aaatcagata aggctcgagg aaatgaaatt
    13981 acatatgcgc cgcgattaac cgttggttat cctggtacat tgcgaacagg tagttgttat
    14041 aactcttccc tttggtcgag tagcccttca gcttgtgaat catcgctgtg gcagtcactt
    14101 cctgatccgc tttacgcagc tgtgcacgcg atttacggaa cgaagagtaa gccgggtgcg
    14161 tattcaggtt agtgacatag gcgctcaccg attctttgac agaactaaac tgtgagtacc
    14221 ctttcacttt acccggcgca ttggtacaac gtcctttcat gcatttcatg ccgaacagat
    14281 tgttattgtt gcgcgccagc ttcgacgttc cccaaccgct ttctgctgca gccatcgtcg
    14341 ccaccatact ggtagggata atgtctacgc gttcaagcaa ggtattccac gggatttttc
    14401 gcgtattacc agaccacttc accttgtagc gtttggcgat gtctttcaga cgcgcacgct
    14461 cagcaggtgc ccattggccc tgatactgtt ttgaaatcag ccagttacgt tctgcagtaa
    14521 tggctgcatt ttggctggta atgtaaggca ttacggtccg gagaaacgcc tttttccttg
    14581 gtgttccgga agggtatttt cgcaaatcag gaagtgaact actctttgca ctattgcgag
    14641 aatactcttg tttactgctt acctgtttat tactagcttt agttaagtgg gacttttgac
    14701 tcgctgttgt tgtgtgcgtc ttcgctaaca cctcactcga aaacaccaga gtgagtaaca
    14761 taagaatcat cgccccatat cgtcgtatgg gagtcaaaat catcaggtct cctggtcgga
    14821 tttaatcatt ccaacacctt atatttttca caaatttgag agttgaatct caaatcatat
    14881 caaaaatagc tgtcaagagc accccaagga atagtccaaa tctgaaacta tgtcacgagt
    14941 taacgattca gattggcgct aaatcgcaga aaatgtgggg gttatcgcaa aattcagccg
    15001 ttttttgcgc gcgatcgctc acccttgctt ctcatcctgt ggacttaccg ctcagggatg
    15061 agttttgtct ggcttatcgc tggcaaactg tctgaaatcg cagcaataag gactcatcca
    15121 ccatgaaact cgccgcctgt tttctgacac tccttcctgg cttcgccgtt gccgccagct
    15181 ggacttctcc ggggttccct gcctttagcg aacagggaac gggaacattt gtcagccacg
    15241 cgcagttgcc caaaggtacg cgtccactca cgctaaattt tgaccaacag tgctggcagc
    15301 ctgcagatgc gataaaactc aatcagatgc tttccctgca accttgtagc aacacgccgc
    15361 ctcaatggcg attgttcagg gacggcaaat atacgctgca aatagacacc cgctccggta
    15421 cgccaacatt gatgatttcc atccagaacg ccgccgaacc ggtagcaaac ctggtccgtg
    15481 aatgcccgaa atgggatgga ttaccgctca cgctggatgt cagcgccact ttcccggaag
    15541 gagccgccgt acgggattat tacagccaac aaattgcgat agtgaagaac ggtcaaataa
    15601 cgttacaacc cgctgctacc agcaacggtt tactcctgct ggaacgggca gaaactgaca
    15661 catccgcccc tttcgactgg cataacgcca cggtttactt tgtgctgaca gatcgtttcg
    15721 aaaacggcga tcccagtaat gaccagagtt acggacgtca taaagacggt atggcggaaa
    15781 ttggcacttt tcacggcggc gatttacgcg gcctgaccaa caaactggat tacctccagc
    15841 agttgggcgt taatgcttta tggataagcg ccccatttga gcaaattcac ggctgggtcg
    15901 gcggtggtac aaaaggcgat ttcccgcatt atgcctacca cggttattac acacaggact
    15961 ggacgaatct tgatgccaat atgggcaacg aagccgatct acggacgctg gttgatagcg
    16021 cacatcagcg cggtattcgt attctctttg atgtcgtgat gaaccacacc agctatgcca
    16081 cgctggcgga tatgcaggag tatcagtttg gcgcgttata tctttctggt gacgaagtga
    16141 aaaaaacgct gggtgaacgc tggagcgact ggaaacctgc cgccggacaa acctggcata
    16201 gctttaacga ttacattaat ttcagcgaca aaacaggctg ggataaatgg tggggaaaaa
    16261 actggatcag aacggatatc ggcgattacg acaatcctgg attcgatgat ctcactatgt
    16321 cgctggcctt tttgccggat atcaaaaccg aatcaactac cgcttctggt ctgccggtgt
    16381 tctataaaaa caaaacggat actcacgcta aagtcatcga aggctttaca cctcgcgatt
    16441 acttaaccca ctggttaagt cagtgggtcc gcgactatgg gattgatggt tttcgggtcg
    16501 ataccgccaa acatgttgag ttgcccgcct ggcagcaact gaaaaccgaa gccagctccg
    16561 cgcttcgcga atggaaaaaa actaaccccg acaaagcatt agatgacaaa cctttctgga
    16621 tgaccggtga agcctggggc cacggcgtga tgcaaagtga ctactatcgc cacggcttcg
    16681 atgcgatgat caatttcgat tatcaggagc aggcggcgaa agcagtcgat tgtctggcgc
    16741 agatggatac gacctggcag caaatggcgg agaaattgca gggtttcaac gtgttgagct
    16801 acctctcgtc gcatgatacc cgtctgttcc gtgaaggggg cgacaaagca gcagagttat
    16861 tactattagc gccaggcgcg gtacaaatct tttatggcga tgaatcctcg cgtccgttcg
    16921 gtcctacagg ttctgatccg ctgcaaggta cacgttcgga tatgaactgg caggatgtta
    16981 gcggtaaatc tgccgccaac gtcgcgcact ggcagaaaat cagccagttc cgcgcccgcc
    17041 atcccgcaat tggcgcgggc aaacaaacga cactttcgct gaagcagggc tacggctttg
    17101 ttcgtgagca tggcgacgat aaagtgctgg tcatctgggc tgggcaacag tgacttttcc
    17161 ggcttcccgt tcgtcagtac ctcgggaagc cgccaaccag gataaaatgt cagccctaat
    17221 cagcgttgcg ggataaagca ccgctcactc ttcaacagac cgatttgcac cccagcaaat
    17281 gtagcgttat tgttaccttc cttgctacag agttcgacag atatcccgct atgacattct
    17341 ccctttttgg tgacaaattt acccgccact ccggcattac gctgctgatg gaagatctga
    17401 acgacggttt acgcacgcct ggcgcgatta tgctcggcgg cggtaatccg gcgcagatcc
    17461 cggaaatgca ggactacttc cagacgctac tgaccgacat gctggaaagt ggcaaagcga
    17521 ctgatgcact gtgtaactac gacggtccac aggggaaaac ggagctactc acactgcttg
    17581 ccggaatgct gcgcgagaag ttgggttggg atatcgaacc acagaatatt gcactaacaa
    17641 acggcagcca gagcgcgttt ttctacttat ttaacctgtt tgccggacgc cgtgccgatg
    17701 gtcgggtcaa aaaagtgctg ttcccgctgg caccggaata cattggctac gctgacgccg
    17761 gactggaaga agatctgttt gtctctgcgc gtccgaatat tgaactgctg ccggaaggcc
    17821 agtttaaata ccacgtcgat tttgagcatc tgcatattgg cgaagaaacc gggatgattt
    17881 gcgtctcccg gccgacgaat ccaacaggta atgtgattac tgacgaagag ttgctgaagc
    17941 ttgacgcgct ggcgaatcaa cacggcattc cgctggtgat tgataacgct tatggcgtcc
    18001 cgttcccggg tatcatcttc agtgaagggc gcccgctatg gaatccgaat atcgtactgt
    18061 gcatgagtct ttccaagctg gggctgcctg gctcccgctg cggcattatc atcgccaatg
    18121 aaaaaatcat cactgccatc accaatatga acggcattat cagcctggca cctggcggta
    18181 ttggtccggc gatgatgtgt gaaatgatta agcgtaacga tctgctgcgc ctgtctgaaa
    18241 cagtcatcaa accgttttac taccagcgtg ttcaggaaac tatcgccatc attcgccgct
    18301 atttaccgga aaatcgctgc ctgattcata aaccggaagg agccattttc ctctggctat
    18361 ggtttaagga tttgcccatt acgaccgagc agctctatca gcgcctgaaa gcacgcggcg
    18421 tgctgatggt gccggggcac aacttcttcc cgggactgga taaaccgtgg ccgcatacgc
    18481 atcaatgtat gcgcatgaac tacgtaccag agccggagaa aattgaggcg ggggtgaaga
    18541 ttctggcgga agagatagaa agagcctggg ctgaaagtca ctaaaaattg cctgatgcgc
    18601 tacgcttatc aggcctacat gatttctgca atatattgaa tttggaagaa tttgtaggcc
    18661 ggataaggcg tttacgccgc atctggcata aacaaagcgc actattccgg cattgagaaa
    18721 cgccggaaaa cgtttcaaac aggctgctgc tgtagccgat gccgctgcac tttctcgaca
    18781 tccatgcact gcaacgcctt tgtcgggcag gcttcaacac aggccggccc cgtctcccga
    18841 tgccagcaca gatcgcactt aatcgccctc gcctttttgc gcgaagaaac gacctccatc
    18901 gcaccaaacg ggcaagccag catacagctt ttacagccaa tgcaacgtgt ttgttcaacg
    18961 aaaatatgcc catgttcgcg gcttatcgcg tcaacagggc agacattcgc gcacggtgca
    19021 tcttcacact gatgacaggc tactggcgtg gtccagctgt ggtctttaat gacacgaata
    19081 cgggaaataa actcgtctgg tgacaacgca gcgcaatcct gattctcctg atgcgacact
    19141 gcgcaagcca cttcacaggt acggcaaccg atacatttcg tcgcatccgc aataataaac
    19201 cggttcatga tcattccttt gcactaaaac agcagagtgc cagagagcgc gtccctactg
    19261 ctttgatcca acgaaggaaa tgttaaagtt ttggcacttg tccaaagcgt tatgtgatcg
    19321 cgcccaggtc atccctgaca gtaaatccca gttcattaga aatagcctgc gcggtttcac
    19381 gcagtggttt caggagattt ttctctccca cctgtttcag acgtgatgtc gaaagcgaaa
    19441 tcgacacggc gtacggcacc cgcccatgaa tatcaaacac cggaacagca atacaggaga
    19501 cgccgagttc gttttcttct ctgtccatcg ccgctccgct ttcacgaatg tgcgccagtt
    19561 cgtcgaacat cgcgggcagc tcggtaatgg tattgcgggt taacggctgg atctcatgct
    19621 gatggttttc ccagtatgac ttcacgtagt ccgggtgacc aaacgccatg tagatcttgc
    19681 ccattgcgga acagtagagc ggcatatgct ggccaatata ggcacgggtt cgcagcatcc
    19741 cggttgtggg ttccagctta taaatcaaaa tagcgtgatc gtcttcgcgg ctggagaagt
    19801 taatggtttc accagtggcg atgttcagtg cctcaagatg cggagcggcg atatgaatga
    19861 tattcagcga agacagcgcc ttctgcccga cggcaataaa tttggtagtc aggcggtaac
    19921 tccctgcggc gggcgcggtg gtcacatagc cgcaggactg taatccctgc aataagcgat
    19981 ggacggtact cttatttaaa ccagccagct ccgaaagatg cgccaacgga caaccgtttg
    20041 gatagttgct caaaatctca atcagcatca acccgcgaaa cagactctgg cttccggctg
    20101 gacgctcttt ttcctgcgcc atctcgttct ctttttttcc catcacttct ttccccattt
    20161 tgtcgcgtcc tgatggtagc gcaaagtgtg ccgtagttca cgatctcgac agataattta
    20221 taaccacttg atttttatgt cttttgaaat tcatcaatca gattgctttg ttaaaaagtg
    20281 atcgatatat ttgaaatcta gttccgcata ttgaaatttt aagccaaaaa agcgatcaaa
    20341 aaacaaggaa gcctaggatg aaagtgacat ttgagcagtt aaaagcagcc tttaatcggg
    20401 tcttaatttc acgcggcgtt gacagcgaaa cggctgacgc ctgtgcagag atgttcgccc
    20461 gcaccaccga atccggcgtt tattctcacg gcgttaatcg tttccctcgt ttcattcaac
    20521 aactggaaaa cggcgatatc attcctgatg cccaacccaa acgtataacc agcctcggcg
    20581 caattgaaca gtgggatgcc cagcgttcga tcggcaacct gacggcgaaa aagatgatgg
    20641 atcgcgccat tgaactggct gccgatcacg gtattggtct ggtggcactt cgtaatgcca
    20701 accactggat gcgcggcggc agctacggct ggcaggcggc ggaaaaaggc tatattggca
    20761 tttgctggac caactccatc gccgtaatgc cgccgtgggg cgcaaaagag tgtcgcatcg
    20821 gcactaaccc gctgatcgtc gccattcctt ccacgccgat caccatggtc gatatgtcga
    20881 tgtcgatgtt ctcttacggc atgttagaag ttaaccgcct ggcaggccgt cagctcccgg
    20941 tcgatggtgg ctttgatgat gagggcaatt tgaccaaaga acctggcgtt atcgagaaga
    21001 atcgccgcat tttgccgatg ggctactgga aaggttctgg catgtcgatt gtgctggata
    21061 tgatcgctac tctcctttcc gacggcgcat cggttgccga agtcacccag gacaacagcg
    21121 acgaatacgg catttcacaa atttttattg ccattgaagt ggacaagctt atcgacggtc
    21181 ccacccgcga tgccaagctg caacgtatca tggattacgt tactaccgct gaacgcgctg
    21241 acgaaaacca ggccatccgc ttacccggcc acgaatttac taccctgctg gccgaaaacc
    21301 gccgtaacgg catcactgtt gatgacagcg tgtgggccaa aatccaggcg ttatgaggag
    21361 ataagtcatg atttttggac atatcgcgca gcccaatccg tgccgtttgc ccgccgccat
    21421 tgaaaaggcg ctcgattttc tgcgcgctac caacttcaac gtcctggagc cgggcgttgt
    21481 cgaaatcgac ggcaagaata tttatgcgca
//
LOCUS       DAZPCS010000054        20780 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZPCS010000054 DAZPCS010000000
VERSION     DAZPCS010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20780
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.054"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..579
                     /locus_tag="ACHUQ7_002010"
     CDS             <1..579
                     /locus_tag="ACHUQ7_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR1135431.1"
                     /translation="GYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGVLNAM
                     ASQRAKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATKML
                     FSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQLVS
                     LPDFSHYELYDKPEAVQEALAKVIPFFNTHLG"
     gene            597..1235
                     /locus_tag="ACHUQ7_002011"
     CDS             597..1235
                     /locus_tag="ACHUQ7_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR1135432.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFVEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            1526..2254
                     /gene="mpaA"
                     /locus_tag="ACHUQ7_002012"
     CDS             1526..2254
                     /gene="mpaA"
                     /locus_tag="ACHUQ7_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFR1135433.1"
                     /translation="MTVTRSRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHNELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="ACHUQ7_002013"
     CDS             complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="ACHUQ7_002013"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFR1135434.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            3313..3819
                     /gene="tpx"
                     /locus_tag="ACHUQ7_002014"
     CDS             3313..3819
                     /gene="tpx"
                     /locus_tag="ACHUQ7_002014"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFR1135435.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="ACHUQ7_002015"
     CDS             complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="ACHUQ7_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFR1135436.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(5552..6613)
                     /locus_tag="ACHUQ7_002016"
     CDS             complement(5552..6613)
                     /locus_tag="ACHUQ7_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFR1135437.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTVRLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(6610..8007)
                     /locus_tag="ACHUQ7_002017"
     CDS             complement(6610..8007)
                     /locus_tag="ACHUQ7_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFR1135438.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDALLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWDESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AAMKADHVTVDQHANMVSLLQQLIQDAWQNAAFERISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGALLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            8163..9161
                     /locus_tag="ACHUQ7_002018"
     CDS             8163..9161
                     /locus_tag="ACHUQ7_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR1135439.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(9272..10144)
                     /gene="ompG"
                     /locus_tag="ACHUQ7_002019"
                     /pseudo
     CDS             complement(9272..10144)
                     /gene="ompG"
                     /locus_tag="ACHUQ7_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006175591.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
     gene            complement(10217..11298)
                     /locus_tag="ACHUQ7_002020"
                     /pseudo
     CDS             complement(10217..11298)
                     /locus_tag="ACHUQ7_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(11312..11971)
                     /gene="pgmB"
                     /locus_tag="ACHUQ7_002021"
     CDS             complement(11312..11971)
                     /gene="pgmB"
                     /locus_tag="ACHUQ7_002021"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFR1135440.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(11968..14235)
                     /gene="ycjT"
                     /locus_tag="ACHUQ7_002022"
     CDS             complement(11968..14235)
                     /gene="ycjT"
                     /locus_tag="ACHUQ7_002022"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFR1135441.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGAGYTIASRRFVSADQLPLIALEITIMPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     TQLHAGETVTLQKLVWIDWRDDRQAALDEWGSVSLRQLEMCAQQSYDQHLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHGERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMVYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMTKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSSASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LLEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLDGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(14232..15286)
                     /locus_tag="ACHUQ7_002023"
                     /pseudo
     CDS             complement(14232..15286)
                     /locus_tag="ACHUQ7_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
     gene            complement(15296..16071)
                     /locus_tag="ACHUQ7_002024"
                     /pseudo
     CDS             complement(15296..16071)
                     /locus_tag="ACHUQ7_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
     gene            complement(16089..17141)
                     /locus_tag="ACHUQ7_002025"
     CDS             complement(16089..17141)
                     /locus_tag="ACHUQ7_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFR1135442.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(17172..18014)
                     /locus_tag="ACHUQ7_002026"
     CDS             complement(17172..18014)
                     /locus_tag="ACHUQ7_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFR1135443.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALLERFIKSGLTAG
                     GVKG"
     gene            complement(18001..18882)
                     /locus_tag="ACHUQ7_002027"
     CDS             complement(18001..18882)
                     /locus_tag="ACHUQ7_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFR1135444.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(18903..20186)
                     /locus_tag="ACHUQ7_002028"
                     /pseudo
     CDS             complement(18903..20186)
                     /locus_tag="ACHUQ7_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(20200..>20780)
                     /gene="ycjM"
                     /locus_tag="ACHUQ7_002029"
     CDS             complement(20200..>20780)
                     /gene="ycjM"
                     /locus_tag="ACHUQ7_002029"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HFR1135445.1"
                     /translation="VNWKNNPDGTRSPYEMNVTYMNALSRRESSDEERCTRFILAHAI
                     LLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKYHSEEITRELNDEATLRHAV
                     YHELSRLITLRRSHNEFHPDNNFTIDTVNSSVMRIQRSNADGNCLTGLFNVSKNIQHV
                     NITDLHGRDLISEVDILGNEITLRPWQVMWIK"
BASE COUNT         5071 a         5669 c         5052 g         4988 t
ORIGIN      
        1 ggctatgcca ttaatgccgc gctgacagaa aaacgaatta aagctgtagt gagtattacg
       61 ggtgttaata tcgggcgtct ttttcgggaa ggtttcagca attatgatcc gattggcgta
      121 ttgaatgcaa tggcgtcaca acgagcaaaa gaggctcgtg gtggagaatt acagatcaac
      181 gaattacttc ccgccagcct ggatgcagca aaagcccatg gactgaccga acgcgatgtt
      241 tatgaagcaa ctgactacta taaaacacca cgcgggcaac aaccgggagg tgcgacaaaa
      301 atgttgttct cacatgcaca gaaaacgctg gcctgggatg ccttcgcatt tactgaagtg
      361 ttactgaccc agccggtgat ggtagtagtt ggtgaaaaag tcggtgcctt cggtgcttat
      421 cgtgatggac tggaagtcta tggccgggcg atggtttcgc aggatcgtca gttagtttcg
      481 ttacccgatt tctcacatta tgaactatat gacaaaccgg aagcagttca ggaggcgctg
      541 gcaaaagtta ttcctttttt caacacccat ctgggataag tttcatgaga aatattatga
      601 aaaatgtact gattcttggt gctggcggtc agatagcccg ccatgtgatt aatcaactgg
      661 cagataaaca aactattaaa caaactttat ttgcccgtca gccagcaaaa atacacaaac
      721 cctatccaac taatagccag attattatgg gggatgtact gaatcatgca gcactgaagc
      781 aggccatgca gggacaggat attgtgtatg ccaaccttac gggggaagat ttagatattc
      841 aggcaaattc agtgatcgct gcgatgaaag cctgtgatgt taaacgtctg atctttgttc
      901 tttccctcgg tatttatgat gaagttccag gaaaatttgt cgaatggaat aatgcagtta
      961 ttggtgaacc cctaaaacca ttccgcaggg ctgcggatgc aattgaggct tctggactgg
     1021 aatatacaat tcttcgccct gcctggctga ctgatgaaga tattattgat tatgagctta
     1081 cctctcgcaa tgaaccattc aaaggtacga tcgtatcacg taagagtgtc gcagcattaa
     1141 ttacagatat catcgataaa cctgagaaac acattggtga gaatataggc atcaatcaac
     1201 ccgggactga tggcgataag ccattcttca tgtaaatcaa ttatatagat accatcgtac
     1261 tgtactatgt gcagtacgat ttattaaact tattaaggtg aactgatcaa tcagatgcga
     1321 ttaacatgtg tagaaccagt aagaataaaa tacgccatgt atataacatt acgacaattc
     1381 taatatctgc acatcaatga aaatgcaaac aacttccggc aaaaaagtga tacaagatcg
     1441 atgacatagc gttgaagaaa aggatttgat aatccgcaaa atttgcacaa cactgcctgt
     1501 aatacttcct gaaccagaga cgatcatgac cgtaacccgc tcacgcgccg aacgcggcgc
     1561 atttccgccc ggaacagaac attacggacg ttcattattg ggcgcgccgt taatctggtt
     1621 tcctgcccct gcagccagcc gcgaaagtgg tttgattctg gctggcactc acggtgatga
     1681 aaactcttcg gtcgtcacgc tctcctgtgc tctgcggact ttgacacctt ctttgcgccg
     1741 ccatcatgtg gtgctgtgtg tgaatcctga cggctgccag ctggggttac gggccaatgc
     1801 taatggtgtg gatttaaaca gaaactttcc ggcggcgaac tggaaggaag gtgaaacggt
     1861 ttatcgctgg aacagcgccg ctgaagaacg tgatgtcgta ttactgacag gtgacaaacc
     1921 tggctccgaa cctgaaaccc aggcgctgtg ccagcttata caccgcattc aacctgcctg
     1981 ggtggtctca ttccacgatc cactggcttg tattgaagat cccagacaca acgaattagg
     2041 cgaatggctg gcccagtcgt ttgaattgcc tctggtaacc agcgtcggtt atgaaacgcc
     2101 tggctctttc ggcagttggt gtgcagatct caacttgcat tgcatcaccg cagagtttcc
     2161 gcccatctcc tccgacgaag ccagcgaaaa atatctcttt gcgatggcaa acttgctgcg
     2221 ctggcatcct aaagatgcaa ttcgcccgtc gtgaactgaa gcgccggttc cacatctacc
     2281 gccagccagg tcggtccgtc aagatcggcg aaactgacct gcggcaccag cggtaaagcg
     2341 gcgctaatgg cacgagaggt acacaacatg cagcccagca tcagactgaa accttgtgca
     2401 cgcgcttcag tcgccagcgc cagcgcttcc gtcagaccac cggttttatc gagcttaatg
     2461 ttaaccatct catagcgccc tttcagcgcc ttcagattgc tacgagtatg acaactttca
     2521 tcagcacaaa tcggcaacgg atgaataaaa ttctccagcg ccgcatcgtc ctgcgcagga
     2581 agcggttgtt caagcatcgc aacgcctaaa tccgccaata gctggcaacg cgccgccaac
     2641 ccttctgcac gccaggattc atttgcatca acgatcagcg tggcatcggg cacagcggtg
     2701 cgaattgcca ccatccgctc actgataaga tggttatcca gcttcacttt cagtaatttc
     2761 gcgcctgcct gccagagtgt tgatgcacta ttggccatct gatcaggcgt accgatgaca
     2821 accgtctgtg cagtgataac tgtctcggga agcgttatgc cgatcaaatc agccagcgat
     2881 tgctgctgtt ttcgcgccgc gagatcccac aatgcacaat ccagcgcatt acgtgctgcg
     2941 ccggcaggga gaattttttg caactcctcc cgtgtcagcc ctttctctaa ttgcggcacg
     3001 acgctcataa tttgcgccat taccgaggca tcactttctc cataacgcgg atacggcgtg
     3061 cattcgccgg tgcctttaat accctcttct tccagttcaa ccaccaccac gcgcgcttca
     3121 ctgcgacttc cccgggcaat cacaaacggg gtatgtaagg gccaggcttc ctcaaatacc
     3181 ttaacgattc tcatctttac tccttgttgt cgcggtggtc tgcggattaa gcgcaaatag
     3241 cgtttgctgt gttattgaca gttagcataa actaggtgtg acgttaacta tatgtaaaca
     3301 ggaaagataa tcatgtcaca aaccgttcat ttccagggca acccggttac agtcgccaat
     3361 tccatcccgc aggcgggtag caaagcgcag acttttactc tcgtggcaaa agatctgtct
     3421 gacgtcaccc tcggtcagtt tgcgggtaaa cgcaaagtgc tgaacatttt cccgagtatt
     3481 gataccggtg tttgcgccgc atcagtacgt aagtttaacc aactggcaac tgagatcgac
     3541 aacaccgttg tgctgtgtat ctctgccgat ctgccgttcg cccagtctcg tttctgcggc
     3601 gcagaaggtc tgaacaacgt tatcaccctc tccactttcc gtaacgctga atttctgcaa
     3661 gcctacggtg tggcaattgc tgatggccca ctgaaaggtc tggcagcgcg tgccgttgtg
     3721 gttattgacg aaaatgacaa tgtgattttc agccagctgg tggatgaaat caccaccgag
     3781 ccggattacg aagcagctct ggctgtactg aaagcataag ttaatatgcc tgatggtgtt
     3841 gcaccatcag gcatattcgc gcttactctt cgttcttctt ctgactcaga ccatattccc
     3901 gcaacttatt ggcaatcgcg gtatgtgaaa cgccgagacg ttttgccagt ttgcgcgtgc
     3961 tgggataatt gcgataaagc tgggttaata ccgagcgttc aaaacggctg gtgatttcgt
     4021 ccagcgaacc ttccatcgca tcttcgccca cggctaccgt tgcggcgtca taatccggca
     4081 acaaaatatc ctgtggacgc agctcataac cgtccagttg tgtcagtgcg cgatagatag
     4141 cgttctttaa ctgccgcaca tttcccggcc acgcataacg tgtaagtaca gtattcaggt
     4201 cagcggccag tttcggacgc ggcacgccct gctcgtcggc aaagcgggcg acgaacagct
     4261 cagttaacgg cataatgtcc tgcggacagt cacgtagcgg cggcagattg agcgtcaaca
     4321 cgttcagacg ataatagaga tcttcacgga acacgccttt ttgcaccagt tcgaccagat
     4381 tcttctgcgt agcgcaaatc acccgcacat cgacatgcac ctcatggtct tcgccaaccc
     4441 gacggaaagt gccatcatta agaaaacgca gtaatttcgc ctgcatccgt ggtgacattt
     4501 cccctatttc atccaacagc accgaaccac cgttcgcctg ctcaaagaac cctttcttcc
     4561 cttccggagc atgaccaaac agttcactct cgaccgcatc ttctggtata gacgcgcaat
     4621 tcagcgccag gtaaggtttg cccgctctgg ggcttgcctg atggcaggcg taggcaaaaa
     4681 gatctttacc tgtacctgtg tcacccgtaa tcagcagcgg cgcgcttagc atcgccagtt
     4741 tctgcgcctg ttcgacaacg tgcttcattt tcgggctgac ggcgacaatt tggctgaagg
     4801 cgctgacgtc ctgggcggcg acattttgca actggcggcc catacgaatc gttgatcgca
     4861 acatcaccac cgcaccggtc aggacgtgtt gatcattttc atcctgaaga taaacaggcg
     4921 taatctccat caggaaattc tgcccattaa taacgacatg ctcgttatgc gaatcttgcg
     4981 gttcgctttc cagccaacgt aaaaaattaa agccgttaat caattgtgcg gcggtatggt
     5041 tgcgcaggcg atccaatttt tgcccaaaaa gctgacagct cgccgggttc gccatatcca
     5101 ctttgctttt catatcgaca gagagcacag gttcaggcaa cgcctccagt aacgcgctca
     5161 acgccagatg ctcacgttcg gaaggcatcc acgggacagt acgcacatcg gtaacacctg
     5221 caatacggcg tatttcggcc atcagactgc tgaaactctc aaactccagt tcagcaaaat
     5281 tgaggtagat tcgcccaatg ggatcaatct caataccgcg taaatcaatg cctcttagca
     5341 cgagtagatc gagtaattcg cgggtcagac cgagtcggtc ttcacaaaag acttccagac
     5401 gcatgggaac cttcacctga aaaaagaaca attaatatga tgatatgaca ctatttggat
     5461 agcaggaagg tttctgtcaa caatcattga cacaaagacg gaaaaacggg cacttcggcg
     5521 taaagatatc cggtcagcac ccgatattgc attatttttc gctgggcgtt ttgccttttt
     5581 gcagagtttc tttcacctga ccgataagct gacgacggaa atccccaagg cgaggtttgt
     5641 catcgtcaat ccacggcagc gggcggcaaa gctccatagc tttaatcccg agtcgtaccg
     5701 tcagaagtcc agcaccaatt ccctgagctg cgcgggtaga caaacgagca gcgagatctt
     5761 gcgacatcca gtccatcccc acttcgcgca ccagttcgct ggctccggca aaagcgatat
     5821 tcagcaatac cagcttaaac agacgcaaac ggctgtaata ccccagttca atgccataca
     5881 gcgtggcaat gcgattaatt aaacgcagat tgcgccaggc gataaacgcc atatcgacca
     5941 acgccagcgg gctgaccgca atcatcaacg ttgattccgc cgccgaacgg ctgatttcgc
     6001 gccgcgcctg ggcatctaaa actggctgga ccaaatgcgc atacaggctg accacttcac
     6061 gatcgttctg cgtttcatgg attgaggcat accagcgttg cagcgccgga tgcgactgat
     6121 caatacccgc ctgctgcgcc agtttttcgc aaaatgcgcg acctttgccc gtgccgtggc
     6181 tatgcaataa atcacgcgct tcgtcgcgtt catgggcgcg ctgtcgcaag cgccataagc
     6241 gccgccactc tgttaccaca gaacctacgc cagcgccgat aatcaatgcc ccagcggcac
     6301 atccacccag cgctacccag tcctgggttt gccaggcatt cattgtccac tgtataccct
     6361 gcccgacaac gcttgcgcca aacagagcca gcccgcccat caccattttg cgccacaggc
     6421 tgcgtttcgg acgtaacgct gcgtccatta ccgcttcgac ttgcccctct tcttcctgcg
     6481 cttcgtcgag cgtggccggg gcaaaatttt gcgcctgatt ttcgtcaaag gtctgctgcg
     6541 ccctgaattt tggattctgt tcgacctcca gcggaccgtc gaaatcaata cgtggtttta
     6601 acggttcggt catcgcaatt tatctcctat taaaaattcc agcgcggcat caagacgaat
     6661 atgcggcagc ggtttgtcga catccatcac ctgcgggcga aacgcttcaa actggaaccc
     6721 ttgcttgtcc cagaacgcct gaccgggcaa acgcgcagga acttcaccgg ggtaaacggt
     6781 gagaagcgca ccatcgctga gccggttacc gcgcaatgcc ggaattttct cgccgttgac
     6841 gtcaataatg ccgctggtgg tcgcctgcac cgaggccagc cccaggcaat ccatactaat
     6901 cctttcaaac gccgcatttt gccaggcatc ctgaatcagt tgttgcagta atgaaaccat
     6961 attggcgtgc tgatcgaccg ttacatggtc cgctttcatc gcggcaaaca gcagtttgtc
     7021 gataaccggc gagaataagc ggcgaaacag cgtgcgctgc ccgtagtgga aactttgcat
     7081 cagctgcgtc agtgccagac gcatatcatt aaatgcctgt ggcccactgt tgagaggttg
     7141 caggcaatcc accagcacaa tctggcggtc aaagcgcaga aaatgattct tatagaaccc
     7201 cttcaccact ttctcgcagt aataattaaa ccgctcgcgc agcattccgg cattggtgtg
     7261 cttatcggcc tgcgccagtt tggactcgtc ccaggcatcg acatccggcc acgggaagaa
     7321 ttgcagcgcg ggcgcacctg ccatatctcc cggcaagaca aagcgcccag gctgaataaa
     7381 gtgcagcccc tgctgtttac agtggtggag ataatcggtc cacacggcgg caatgtctgc
     7441 cagacggttt tcgtcggcag gcgctagcgg gtcaagtcct tcgcacatca ttcgccattt
     7501 cgccgaccat tcgccgcgct gaccattgag taagcccgtc atctggcgcg accagcttaa
     7561 gtaatcctgc gccagcatcg gcaggtcgag caaccattcg ccgggataat ccacaatttc
     7621 cagatacagc gtggaggtat ccttaaagtg gcgcagcagc gcatcgttcg atttaaaacg
     7681 taacgccagg cgaatttcac tgacgccgcg cgtaggcgtc ggccaggcgg gaggatcacc
     7741 atataactgc gccagtcctt catcataggt gaagcgcgga atgccaaagt cacgctgggg
     7801 aatgcgtttt acgcccagca agcgctcttc acgcaccgcg cttaacagcg gcaaacgtgc
     7861 tccggcatga atattgagca actgattaac catcgcggtg ataaacgctg ttttgccgct
     7921 gcggctaagt ccggttacag cgaggcgcag atgtctgtcg acaccccgat tcaccagcgc
     7981 attgagttca tttttaagtc gcttcattgc tgtcctgtgc tgctctgaag accagaaaaa
     8041 aaatcattcg taaggatatg aatgtggggg ccaggccatt gattatcaac tgcccgtgtt
     8101 gtcatcgacg ggcgaactcc ataagatgtg cagcattcac gttcggacgg agttcctgac
     8161 ctatgtcccc tactatttat gatattgcca gggttgcagg cgtatcaaaa tccaccgtat
     8221 cacgcgtgct gaataagcaa actaatatct ccccggaagc gcgcgaaaaa gtgttacggg
     8281 ccattgaaga attacagtat cagccaaaca agctggcccg cgcgctgacc tcttcgggtt
     8341 ttgatgccat tatggtgatt tctacccgtt cgaccaaaac tacggcgggt aatccgtttt
     8401 tctctgaagt tttgcatgcc atcaccgcca aagcggaaga agaaggtttc gacgtgatat
     8461 tacaaacgtc gcacaatccg gcagaagatc tgcaaaaatg cgaaagcaaa attaaacaga
     8521 aaatgattaa aggcattatt atgctcagtt cgccagcgga tgagtcattt tttgcccaac
     8581 tcgataaata cgatattccg gtggtggtaa ttggcaaagt tgaaggtcaa tatgcccatg
     8641 tttattctgt cgataccgat aattttggtg acagcattgc gttgaccgat gcattaattg
     8701 aaagtgggca tcaaaatatt gcctgcctgc atgctccgct tgatgtccat gtttcggttg
     8761 atcgcgttaa tggctataag caaagtctgt ccgcgcataa tattgcagtg cgtgataaat
     8821 ggattgttga cggcggttat acccatgaaa cagcactgca agcagcacgg caattattaa
     8881 gtcagtcacc gttgcctgag gcagtgtttg ccactgacag cctgaaatta atgagcattt
     8941 atcgtgcggc ggcagagaaa aatattgcta ttccgcagca gttagcggtg gtgggttata
     9001 gtaatgaaac gctgtcgttt attttaacgc ctgcaccagg cggcatcgat gttccgacgc
     9061 aggagttagg tcaacaaagc tgcgagttat tattccgctt aatttcggga aaaccgtcac
     9121 cacagaatat taccgttgcc acgcatatga cgctgaaata aataccagct accttgcctg
     9181 aatgccggat gcgatgctgg cgcatcttat ccggcctaca tttatagatc ggataaagta
     9241 ctcgcgccgc atccggcaat ttagcccatt atcagaacga gtaatttacg ccgacacctg
     9301 cataatggaa tttatcgctg tcgccttcgt cgtgatcctg ccactcaaac gcgtattcca
     9361 gcgaaacgga aagtccgttc tggaaatcat aaccgtaaaa taaacccaca cggttaaaat
     9421 catggccttc acgttcaata tcatcctgcc agtcccagtt actctagcga tccagcccaa
     9481 tgcgcgtata cggcgtcacc gagtggttgc cgagcgtcag cggcaaatag gcgcgaattt
     9541 cttgtgtgga aaactcaccg ttattgcggc tgtcgtccat attgaagccg cgctcgagat
     9601 aatagttcac tcgcaaagca accgtttcgt tgaaggtata ttgcagacct gtttccgttt
     9661 cgacacgggt atcagcgtaa ccggtcgtgt tcaaatcgtt ggcaaattta tacatcgaca
     9721 accaaccgtt gaaacgtaaa tcatcagtca gtttcacatc ccagtctggc gcgattttcc
     9781 agcgctgcat attcgccgtg tctttgcccg gttcatcaac gtagtgataa ccataattac
     9841 ggaaaccgcc ggtcaggccg aaactgaaat catcgctttc gaggaactga taatgcacct
     9901 ccagctccgg gcgatcaaac ctcgttccac gtttacccgc actataatct accggccctt
     9961 cctgataata ggccagagca attctccacg gcccgttggc ggcattaaaa tagactgaag
    10021 gctccgccag cccatccata tcttcgccat aaccctcgac gttttctatt tcgtacatcg
    10081 caccgatatt aaagtgccag tcgctctttt cctcggcctg tgcgcaggcc attcccgcac
    10141 acatcaccag tgcggtacag ggtaataact ttttttgttc cttgtattat cctgggggaa
    10201 tgccttcccc ctgtatttag cgaattgcta tttccgtttc tgcatcaaag aaatgacatt
    10261 tcgtcatatc aaaatgaata gtgatatttt ctcctgcatg ataatcattt aacgcaccag
    10321 cacggaccac taactcgtgc ccccaaccgt ggtgtagagc ataaattccg caccggttaa
    10381 ttctgccacg ctaattttgg cggaaatgtt attttcctct tgcgcgtccg gatgaatatc
    10441 ttccgggcgt attcccatca cgatgggctt atgcaaactt tcctgtgttt tcagaaccgc
    10501 taatttctct tcgagaatgg ttaatttaag cgtttccgta acgaatttat cgccatcgat
    10561 cgtgccgcga ataaaattca tcgctggtga tccaataaat ccggcaacaa acatattcgc
    10621 gggttggtta taaacggttt tcggcgcacc tacttgctga acaatcccgt ctttcataat
    10681 cacaatccgc gccgccatgg tcatcgcttc ggtctgatcg tgggtcacgt agatcatggt
    10741 ggtgttcagt ttctgatgca gcttgctgat ctctgcacgc atttgtacgc gcagcttggc
    10801 atcaaggtta gagagcggtt catccattaa aaacacgccc gcttcgcgca caatcgcccg
    10861 cccgagcgcc acacgctggc gttgcccgcc ggaaagcgcg cccggcttgc gtttcaggta
    10921 ctcacgcagg ccgagaattt gcgccgccca gttcacccgc tcatcaatca cctctttggc
    10981 gattttttgc atcttcaggc caaacgccat gttgtcgtaa accgtcatat gcggatacaa
    11041 cgcgtagttc tggaacacca tcgcgatatt gcgtgctttg gctggaacgt cattcattcg
    11101 tttgccgtcg atcaacagat cgccgccgct gatctcctca agcccggcaa tcatgcgcag
    11161 ggtggtcgat ttaccgcagc ccgacgggcc gacaaatacg atgaactctt tatcggcaat
    11221 ttccaggttg aagtccttca ccacatgcac ctggttatcg tagatttttt gaatatgttg
    11281 taacgaaagc tgagccatgt tgattccttt gctatacgtt ttgccagaag gccgataacc
    11341 gcgaccaggt cagggagtcc gttgagggca acagtaattg cgccccggtt aagcccgcac
    11401 cgatccccac cgagcgcata ccgctggcgt taatggcgtc aatgcccgcc tgcgcatctt
    11461 caatgccgat acatgcctgc ggcggcacgc ccagcccggc acaggcagcg agaaagattt
    11521 ccggatccgg tttcgagttt ttaagttggg aagcatccgc gcagaaggtg aaaaactcac
    11581 gcagctccag cgccgctaaa atcgtcggcg cattcaggga gacggaagcc agccctaccg
    11641 gaatctgctg tgcacggagc tctgccagca aattgcgaat accgggcagc acagcattaa
    11701 ccgtcagctc gcgcagtgag tggacgtaaa gcagattttt gcgatacgcc agttgcgccc
    11761 gctcctgcgg gttaaagtcg ccctctttgc ccccgtgttg cagaatgcgc cgcagagact
    11821 catcgcggct gatccctttt agagattcgt taaactgcgc atcaatactg atgccaattt
    11881 cagcggcaat ctgctgccac gcctggaaat gcagatgcgc ggtatcggtg attacaccat
    11941 ccagatcgaa aattacccct tgcagtttca ttcatcctcc tgatgcgtgg tagcggtccc
    12001 attgaagggc aaaataaaat cacccaaaca gaaaacagat tcttcagcca cggatataag
    12061 ctgaccgtcc aaacgcagtg aaacgggcgc agaagttcga atcgcaatac gctgcgcgtc
    12121 gagagtgacc tgtaattcgc agccctgcca gaacagaggg aaagacaact gttgccactg
    12181 ctcaagtaac gccggattta gatgcaattc accgtcacgc acgctcaccc cggcaaaacc
    12241 ctgaatcgcc cccagccaga tagcgccagt tgcggcagca tggataccat catcacaact
    12301 atgcggatca gcaccaagat cgatttcagt cccctcgcgc cagaactgat aactttgggt
    12361 cagcaggcca cagcgtgcag caacaatgcc gtggattgct ttacttaatg acgagtcgtg
    12421 aatagtgcgc ggttcataaa attgcagatt ggcaagacac gatgccgatg agaactgctc
    12481 tggcagcatg taattgagca tcaccacatc agcttgtttg aggatctgca tctcgttcac
    12541 ttctgcgcgt gaataatcca gcagaatggt ttgcttcccc gccgccgctt tgtatttcgc
    12601 cagattaatc gccggcttag tcataaacga atcatcctgc ggcaaaacac cgtcgggctg
    12661 aatttctggc agccgcagtt ctttaaggaa catttcggca cgatgaataa acgcgtcgtc
    12721 actacagcca aactggcggg caatactcag cgcctgctgg acgttgtaat acaccatata
    12781 gctggtgaag gcgttattat tgacatgttc ggtatattcg tctggcccaa taacatcatg
    12841 aatttccaga cggtcgttaa ccctcaccgc gcggctaatc cagaactttg cagtttccag
    12901 aagtagcgcc atgccttcat gcgcaatgaa actttcatcc cccgtggtct gccagtattg
    12961 aataaccgcc caggcgatat cggccaccag atgatgttcc gcctgcgccg aggccacttt
    13021 ttgccgcagc ccggtgcgaa tgttaatggc ggcaaattcc ggcgtctctt cttcgccgct
    13081 gcgcgcgctt tcccacggaa atagcgcgcc ctgccagccg ttgcgtcgcg ctttctcctg
    13141 cgcgcctggc aagttgtgcc agcgataacg cagtaaactt cgggcaaccg tcggatcgct
    13201 aaacagatgg aacggcagca aaaatacttc tgtatcccag aaaacgtggc ctttgtagcc
    13261 ttcgccggtt aagccttttg ccgcaatact gctgcgctcg ccgtgagccg gcgtcatgat
    13321 gcgcagatga taaagcgcat aatctaacgc ttgctgatcg tgcgcatcgc cgccatttac
    13381 cgtgatacgg cgtttctgcc accattgacg ccagttttct gttgatgctg caagatgttg
    13441 gtcgtaactc tgttgcgcgc acatttcaag ctggcgaagc gacacgctgc cccactcgtc
    13501 taaagcagct tgcctgtcat cccgccagtc gatccacacc agtttttgca acgtcactgt
    13561 ctcgcctgca tgaagctgcg tactggtatg ttgcagtaaa cggcgctctt tggcggtata
    13621 gcattgctgc acatcaccgc tcacctggca gcaacaactg atggccacat cactgcgccc
    13681 atcctgggtg gtgtagatcc cctgcatcaa atgctgacca aacacccgca cctgggtttc
    13741 gtcgagatgc tggcgaccgt ggttggtttg cgtggcgtca atgcctgttg aaatcagcac
    13801 tgacgcgtcg gcgtccagtg gcataatagt gatttccagc gcaatgagcg gcagttggtc
    13861 tgccgaaaca aagcgacggc tggcgatggt gtaacctgcg ccgttgctgg tacgccagac
    13921 aacgctacgg cgtaattctc cactggcaaa gtcaagttca cgctgccagg cttcgcggga
    13981 taacgagaaa acctcaccat ttatggcaat ctccatcccc aagatatcag gcagattcac
    14041 cagttcgttg atttcacctt ttcccgcccg atgatacagc cccgccagat acatccctcg
    14101 cgtctggcgc gtgtaatctt cttcatggct ggcgcgaagc ccaagatagc cgttcccctg
    14161 cgccatcagc gatgcatact tgttcagggt atgctggctg aaatggggtt ctgataacgt
    14221 tactggcctg gtcataattc cacacgcgtc cctgtttctg cggattgata taacgccgcc
    14281 accagttgct ggatgatgta cccctgctcg gcgtcggcta tcatcacggg cttgccctgt
    14341 acgtgattga taaaggcttc catgctgcgc aaatggcggt tgtcgtctgc catttcccgt
    14401 tgcatcagcg tcattaattc accgttgtta tcggtgtaga tatgtgctgg aaacagcgtc
    14461 gcaccggctt tatcaccaca aaagctgacg ttcataatca actgttcgcg gatgttgagt
    14521 gcaaatgacg tttccagcca cagaatgccg ccgttatgaa attcaatggt gccaaacagc
    14581 gaatcttcga cgctgtaagt tgccggatcc cactcgccaa attgaccaca gctcttttgc
    14641 gtgccgatct tttgaaaact atgcgcattc acgcttttca ccgccggaaa gcccagcaca
    14701 tacatcgcag catccagcat atgaatgccg atgtcgatca gcaggccacc accctgcagt
    14761 tctttattgg taaagacacc ccagccggga acgccgcagc gacgcagggc gcgggcggtg
    14821 gtaacgtaaa tctctcccaa aacgccgttg gtcacctgtt cacgcagctg ttgcgtatcg
    14881 agcgcaaacg atggtgaaag tcgtaggcca gcaccttgcc cagtttgcgc gcggtatcgc
    14941 acatttccca cgcctgttct gtcgtcatgg cgggcggttt ttcgcacatc acatggcagc
    15001 ctgcttccag tgccatcagg gtatgttcgt aatgaaaacg gttaggtgag ccgacgctaa
    15061 ccacatcagg tttcaccgcc agcagcatgg cctgcggatc gtcccacacg gatgcattcc
    15121 cgtatttttc tgccagcgcc tgcgcctggg aaaggtggct gtcacagaca gccaccagtt
    15181 ccagatcgtt gcgggtgcag tagtacgaag catgaacctt atccgccacc tggcccgcgc
    15241 ctattatcgc gacccgcagc ggagagcttg tcattgcact tttcacaaag acctcttagc
    15301 aggtacgcaa ccaggccaac gaatcacggt acgcctgggc aggatcttct gcccggatac
    15361 gcccttcata caccacataa ccctgatagt tatcggcgcg cagctgttca aacagcgcgt
    15421 ggaaatcgag ggtgccactg cccggctggt aacgatggtt atcggcaata tgcacatgac
    15481 cgagcaagtc acggttgtcg tgcagcgcct gcgccaggtt atcttcttcg atgttcatgt
    15541 gatagaaatc gccgataatc tgcacatgtt taagattgtt ttcgacgatg taacgacgag
    15601 catcggcgag ggtgttgatc atatgatcct gatagcggtt taacggttcg agatacacca
    15661 cggttccggt acgcgcggcg acctgttcca gtacgcgcag ggaatcactc accagtttgc
    15721 ggtcgccgtc caggctacgc ggcgaggtca tcggcggtaa gcggaaggta aacatgcccc
    15781 acgctgccgg aaccccccca cttctgccag cgcctcgaga atgcgttcga tctgctttaa
    15841 gccattaaga cgacgctctt cgataaagtc accaatccag ccgtcatagc cgccgcaggc
    15901 ggtggtcact ggtaaaccgg tttctttgat cgccgctttg acttcttcga tgttgttgac
    15961 cagcagtttg ccgtcaatct caaaaccatc gaagcccatc tctttgatat aacgaaatct
    16021 ctcgcggata ttttccggaa agaatgcctg attctgtgtg ccgattttca ttttttcatt
    16081 cctttgaatt aaaacgtaac gcccattttg atgctctgtt ccggatgctg atcgacatac
    16141 tgcatgtagc tttccgggct gttggcaaag gtcactaccg ggtcgattaa atcttcgcaa
    16201 ttgagataac cgttcatcag cagttcccag caggtttctt caatacgctt acggctccag
    16261 cgcggataat ccgggttcgg ttcgctgcac gcgcgggaga aaacaatttt ggcgttattg
    16321 aaatgcgctt cgcgtccgag gttaaaacct tcagcaaatg gtttggcaaa cgcgacatag
    16381 gagatggtgc cgccgtaggc caggccgcgc agcgccgatt gcagcgcgtc ggcgtaaccg
    16441 ctggtttcga taatcacatc agccccctgc ttgccggtca gcgttttgat ctctttgcct
    16501 acgtcagtgc caatggggtt aaggcagaaa tcagcaccgt ggcgacgggc aatatcacag
    16561 cgatgggcga taggatcgac gccaattacc accgatgcgc cagcgcgttt cgccagttgg
    16621 atggcaattt gaccgatcgc gccaagccct accaccacga caaaatcccc tacgcgtacg
    16681 ttggcatcgc gcacgccgct catggcaaac tgcgccgggt cgtaacagac ggcgtttttc
    16741 caggagctgc cttgcggcat tttgcgcagc ttgtagttat tcactgcgtt aatgatgacc
    16801 gtctcggaga gcgggccgta gccgcatacg ctgtcgccca ccgcgtagtc tgtaacgtcg
    16861 ctgccgcact cgataatgtc gccaaccacc atgttgccaa gctggaattt gccaaactca
    16921 atgccgcgcg gcgcatctgc cggacgcggg gtgaacatct gccactcgcc gttaaagtct
    16981 tcatcgataa acgggctggc ggcgcggaag tcgaccactt ccgttccgtg tttcggtgcg
    17041 ccgaaacgca cgcggatctt cacttcattg gctaaaatcg cccgatcttc atactcaacc
    17101 agcgcagcaa cacgcggtgc tgtggctact aactttttca tgtaaaactc cttgtaaaga
    17161 aactggccgc tttagccctt cacgccaccg gcggtcaaac cactcttaat aaaacgttcc
    17221 aaaagcgcat acataatcac caccggcaat gcggtcacca gcgaggccgc catcatccgt
    17281 ccccagatgt aatcaggcgt actgaacagc gcgttcaggc ccactggtaa ggtgaaattg
    17341 ctggcgctgg agaggaaaat cgacgcaaac agatagtcgt tccacgccac cataaagcag
    17401 tagacgaaca ccgatatcag cccggacatc gccaggggca cggtgatacg gaagatgatt
    17461 tgcagacggt tgaggccatc catcatcgcc gcttcttcga tctcatcagg gatggtgtcg
    17521 aagtaacttt tcagcataaa cacggcggtg ggcagggttt gcgtcaccat ggtaatgatc
    17581 agcgccatct cggtgtcgta aataccaagc gcggtgatga ttttgaacag aggcaccacc
    17641 agcaaaatgc cagagaacat atacaccgtg taaaagctgg cgttgatggt catccggcct
    17701 ttaaagcgca ggcgggaaag cgcgtaagct ccgagaatgc cgagaaatac cgccaccacg
    17761 gaggaaacca ccgacaccac catgctgtta cggaagtagt cgacaaacgg gaaaatcatc
    17821 gggttaaaaa tgtcgacgta atgctccagc gtccactgtt gcggcagcag cgtaggatgc
    17881 agcgatatcg cctctttcgc gcttttgaac gaggtcatca gcatcacaaa aaacggaaac
    17941 agcgtgatga tgagaaacag cgccagcccg cagtaaaaac cgatacgact gagcgtgcgt
    18001 ttatttgttg ccattgaggt tcacccgttt cctggtcagc agaatgacag cgaaaataat
    18061 gatgaagagc accaccgaga tcgccgccgc tttccctaag tcattaaagg caaaggcggt
    18121 tttgtagaga tagacaccaa gaatatcgac cttggttgtc agcagataaa catcagcgaa
    18181 catgtagaac atccagatgg tgcgcagggt caccaccgtc gccaggaccg gcataattgc
    18241 gggcagcgtg acgatgcgaa accgctgcca ggcattagcg ccatccatct ctgccgcttc
    18301 gtacagcgat ttgtcgatgg tctgcaaaat cgccagaaac gagataaagg catacgggaa
    18361 gtagcgccag atggcgaaca gcaccaccag cacaaagcta ctgcccggat tatcaaacca
    18421 cagcggtgcc tgctcataga gatgcagaag atcgacgccg aggtagttca caatgccgta
    18481 gccgttgttg aacatgtatt tccaggcaaa caccagcgaa atggacggcg ttacgtagga
    18541 gaggatcacc agcgaacgcg cggttttgcg caggcggaac tcgcggttga aaaacatcgc
    18601 cacggccagc ccgagcacgg tgctgcccgc caccaccagc gccgtatacc agaccgtcat
    18661 ccacagcgag tgccagaagc cgggatcgga gagaatgcgc acatagttac tcaccccaac
    18721 aaacgttgac tcgatgttgg gattgagcgg cagacgtaaa aaactgattt cgatattcga
    18781 caccatcggc cacgccacca gaccgcccag cagtaataag ctgggcgcga gaagcagcag
    18841 cgcaaacggc atatcggaac gacctgaaaa aagcctgttc attgcggatt cctgtcctgg
    18901 gtttagtgct gttcgatcag ttcatccagc tttttctggc tcgcttgcag cgtgccaggg
    18961 agatcggctt tacccacggt gacgttatgc accatggagc tcaccacacc agaacccgtc
    19021 acatcgccca tgcgggtaaa gtttttatcc cctactgcgc caaagacctg aatatttggc
    19081 agttcaccga ttagctgatt cggtagttca cccagcgcct taataacgtc gttgtctttc
    19141 caggtggcgg tagtgacaac cgctttattc accggcagcg cagcacctgg cgacatcatc
    19201 acccagtcgg caatgttgtc cgcctgctcc ataaaggtga caaatttttc tgctgcttcg
    19261 gtcttttgtc cggcggtaat ggtcagcgag gtcaacatgc cgtagaccgc agagtttttc
    19321 tcggttggca ccacgaaacc gacgtttttc gggtcgcctt ctttaatcac agccggaagg
    19381 atataggtgg agtaaatcgc catcggcgcg gtgccgttca taaaggcgtc tttcacttcc
    19441 atgatgtcgt tagaacccgg catggtgttg gcagcaaggt cgcggtaata ggtcagcgcc
    19501 tgcatcatct ctggtgtatc aagggtgatt ttgccttcgg cgttaaagac gttgccctag
    19561 ttggataacg caaactggga gaaggattgt tccgtcagca cgctttctgc cgtaggcagc
    19621 gcaataccgt attttttatt cgccgggtca ttcagtttct gtgcaacgtc cagcagctgt
    19681 tgccagtttt tcggctcctc cagccccgct tttgccagca catctttgcg ataccagatg
    19741 ccgccaatcc aggcgctgac aggaacaccg gtccatgcgc taccatcttc ggtacgcaca
    19801 atacgcagta cgccatcgta aaacgcgcct tcaccaacgt tgctgatgac cgtggcaacc
    19861 gctttgcgat cgataagctg ctctttatcc ataactttgg cgtagtcatg gctggtttcg
    19921 atcacttccg gcagcgagcc gctacgtgaa agggtgatga ctttggtgtt atgagcgtct
    19981 tcttccacgg gcacttgttt aacggtgata cctgggtttt ctttttcaaa acgggcaatc
    20041 aatttgctga taactgcctg acgctcctgc tccaccgaag aatgcataaa ttcgatggat
    20101 acatgggttt tattttcttc tttacagcct gaaatcaggg cgcaggaaac cagtgctgat
    20161 aacagcacga tttttgattt aatcatgaga tgttcctttt tatttaatcc acataacctg
    20221 ccaggggcgc agcgttattt cattacccaa tatatcaact tcactaatca gatcccgacc
    20281 atgcagatca gtaatattta catgctgaat atttttactg acattaaaca atccagtcag
    20341 acaattacca tcagcgttac ttctttgaat acgcattacg gatgaattaa ccgtatcgat
    20401 ggtaaaatta ttatccggat gaaactcgtt atggctgcga cgaagtgtaa ttaaacgcga
    20461 caactcatga tataccgcat gccttaatgt agcttcatcg ttcagttctc gtgttatctc
    20521 ttcactgtga tattttttac ggttaatcgc acggttatat ccgagttttt cgacacctgc
    20581 gtaatcatta cgcgagccaa gaatactttg aatatatatc gcgggtacac cggggaaact
    20641 taacaaaatc gcatgggcaa ggataaacct ggtgcaacgt tcttcatcgc tactctcgcg
    20701 gcggcttaac gcattcatat aggtgacatt catctcatag ggactgcgcg tgccgtcggg
    20761 attatttttc cagtttacta
//
LOCUS       DAZPCS010000055        20713 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZPCS010000055 DAZPCS010000000
VERSION     DAZPCS010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20713
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.055"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(77..4537)
                     /gene="mukB"
                     /locus_tag="ACHUQ7_002030"
     CDS             complement(77..4537)
                     /gene="mukB"
                     /locus_tag="ACHUQ7_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFR1135446.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLLGRDEAPSQAS"
     gene            complement(4537..5241)
                     /gene="mukE"
                     /locus_tag="ACHUQ7_002031"
     CDS             complement(4537..5241)
                     /gene="mukE"
                     /locus_tag="ACHUQ7_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFR1135447.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(5222..6544)
                     /gene="mukF"
                     /locus_tag="ACHUQ7_002032"
     CDS             complement(5222..6544)
                     /gene="mukF"
                     /locus_tag="ACHUQ7_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFR1135448.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6541..7326)
                     /gene="cmoM"
                     /locus_tag="ACHUQ7_002033"
     CDS             complement(6541..7326)
                     /gene="cmoM"
                     /locus_tag="ACHUQ7_002033"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFR1135449.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     YQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7462..8241
                     /gene="elyC"
                     /locus_tag="ACHUQ7_002034"
     CDS             7462..8241
                     /gene="elyC"
                     /locus_tag="ACHUQ7_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFR1135450.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8218..9111)
                     /locus_tag="ACHUQ7_002035"
     CDS             complement(8218..9111)
                     /locus_tag="ACHUQ7_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFR1135451.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9265..10011)
                     /gene="kdsB"
                     /locus_tag="ACHUQ7_002036"
     CDS             complement(9265..10011)
                     /gene="kdsB"
                     /locus_tag="ACHUQ7_002036"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFR1135452.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHDDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10008..10190)
                     /gene="ycaR"
                     /locus_tag="ACHUQ7_002037"
     CDS             complement(10008..10190)
                     /gene="ycaR"
                     /locus_tag="ACHUQ7_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFR1135453.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10242..11474)
                     /gene="ycaQ"
                     /locus_tag="ACHUQ7_002038"
     CDS             complement(10242..11474)
                     /gene="ycaQ"
                     /locus_tag="ACHUQ7_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFR1135454.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11511..12497)
                     /gene="lpxK"
                     /locus_tag="ACHUQ7_002039"
     CDS             complement(11511..12497)
                     /gene="lpxK"
                     /locus_tag="ACHUQ7_002039"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFR1135455.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHEDVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12494..14242)
                     /gene="msbA"
                     /locus_tag="ACHUQ7_002040"
     CDS             complement(12494..14242)
                     /gene="msbA"
                     /locus_tag="ACHUQ7_002040"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFR1135456.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14279..16538)
                     /locus_tag="ACHUQ7_002041"
                     /pseudo
     CDS             complement(14279..16538)
                     /locus_tag="ACHUQ7_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(16745..17029)
                     /gene="ihfB"
                     /locus_tag="ACHUQ7_002042"
     CDS             complement(16745..17029)
                     /gene="ihfB"
                     /locus_tag="ACHUQ7_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFR1135457.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17189..18862)
                     /gene="rpsA"
                     /locus_tag="ACHUQ7_002043"
     CDS             complement(17189..18862)
                     /gene="rpsA"
                     /locus_tag="ACHUQ7_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFR1135458.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18973..19656)
                     /gene="cmk"
                     /locus_tag="ACHUQ7_002044"
     CDS             complement(18973..19656)
                     /gene="cmk"
                     /locus_tag="ACHUQ7_002044"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFR1135459.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQLIEKALQ
                     YARQKLALA"
     gene            complement(19829..20593)
                     /gene="ycaL"
                     /locus_tag="ACHUQ7_002045"
     CDS             complement(19829..20593)
                     /gene="ycaL"
                     /locus_tag="ACHUQ7_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFR1135460.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         4981 a         5728 c         5031 g         4973 t
ORIGIN      
        1 ccgttatgtg ccattttttt agtgcagaaa cggagttttc ggaaaaagaa aaggcggcat
       61 tgctgccgcc ttaattttaa ctcgcctggg aaggcgcttc gtctcttcct agaagcgttt
      121 cagggagttg cggcgcaaat cctcgcaggc cgacgacgtg aacatgttcg gtattctgga
      181 agactttacg taccagttta taggtggtgc ctttttccgg actgatattt tccggcgctg
      241 cgatgatgag ctgcatttgc aaacgttcac acaattcaaa cagcgtggcg atagaacgag
      301 catccagtcg cgctgcttca tcgaggaaca gcaggcggca aggagagata tctttaccgc
      361 gcaggcggcg agattcatct tcccagcttt gtaccaccat caccagaatc gacataccgg
      421 taccaatcgc ctcaccggtc gacaatgcac cagactctgc gcgcaaccaa ccatcggaac
      481 cacggttaac ctcaacttcc atttccagat agttgcggta atccagcagt tcttcgccaa
      541 tggtctgcgg cgtgcgctgc cccatatcaa tctgcggatt aagacgctga tacaatttcg
      601 ccaacgcttc cgagaaggtc agacggttgc tgttaaacag atcctgatgc tgctcgtgct
      661 gttcagagag cacatccagc agcatggcgt gcgtttcacg cacgttcacg ttgagacgca
      721 cgctgttcac ctgaccaaac gatacgttct gcaacccctg gttgagcata cggatacggt
      781 tctgctcgcg ctgaatggtt ttgcgaatga tgttcgccac gctgcgggaa ctgatcgcca
      841 gtttctgttc acgggaggtt aattcttcgg tcagacggct aagttcaatc tccatctgtt
      901 cgatagcttc caccggatca tcggtacgaa taatatcctg acgaatacgt tcacgcagat
      961 gctgataaac cgccacgaag aactgaattt tacgctccgg acgtttcgga tcttccgaca
     1021 tgcgcagcac gtcgcgcaga tgttcgttat ccgccaccgc cagacgcagc gcacctaacg
     1081 ccttatccga catggaacgc aaatcatcag cggagagata agccagctca cgacggtgta
     1141 agcggcgctc aacgccgtta tctttcacca tgcgcatcac cgcacaccag cccgctttgg
     1201 cggtcactac ctgctcgcgc atctcaaagt aatcccgctc cagcttgcgc agtttgcggg
     1261 tcaggttgtc catctccgct tcgcagaagg taagcgcttt ttccagttga ttgcggcgtg
     1321 aacggttatt gctcagttgc gcatgcagct cgtcacggcg aatacgcgcc cgctcttctg
     1381 ccccgctatc agcacgcacg ccgatatcct gcaattcacg ttgcagatcg ttgagtagct
     1441 cttttttggt gtcgtaagaa cttttcagcg aagccagcac ctggttgtac tgactcaact
     1501 gcgctgcgtg accgcgcaac gcttcgcgag cgcgggtacg ctccgcttcc gcctgttcca
     1561 gacgttcacg cagtttttcg ttgagatcgc tgttaccgct aagcatttct gccgagtcag
     1621 aatagctaaa gtgcgcacga cgctgcacca cttccgtcag ggcaaacgct tgctgacggg
     1681 catcgcgctg catctgctga gagtacgcgt aatcttcttt taactgttcg aactgttccg
     1741 ggtcgctctg caataccgaa acgatcggtt ccagtttcgc cagttgattg ccaaactgct
     1801 gaacaaaacg cgcggcttcc tgggcttcat ccagacgttc gcggatttca tcgacgcgat
     1861 ccgccaggct gtcatcagcc aacaggttga gacgcggcag aatgcggttc agcgccgtaa
     1921 cgccttcttt cgcctgctca aactgaatac gctgctgctg gttatcattt tcatgattac
     1981 tcaacgcccg ctccagttcg actcgacggc tgttcagttg acggatttct gcttccgggt
     2041 cagactcaaa cgccaccgcc agatgactgc cgataaagcg gctgaacgcc tgatgcagac
     2101 gctgggtttt ctgtacatca aaggagagcg tggcgaagcg ttcggaaagc acttcacgct
     2161 cggcatggag gctttcaata cggctttcac gcgcagcacg accaaacagc ggcacttccg
     2221 ggaaacgtga ataacgccac tgtcgatcgg cgattttcac caccaccgct ttttccagct
     2281 catcaacgct gaacacgcta tcatcgaatg actgcggatc cccttcgatc aaatagagat
     2341 cttccgggca atcggtcaag ccttccaggt gttcggttac ctgtgacaga tctggcacca
     2401 cgatggcgtg gcgtgacggg ccatacagag ccgagaagta cggcgcatct tccaggctaa
     2461 cgtcgtcata aatttctgac agcagcacac caccaaaacg ctccgccagc gcgttcagac
     2521 gctgatcttc agagccgcca ggctggctta aacgttcgat ctcttcatcg acggcgtttt
     2581 tgcgcgcgcc tacttcatcg cgttcaacaa tcgcctcgcg ctcacgctcc agcaactgtt
     2641 gcagatattc agtgacgtcc tggctggagg agaactcttc gccgcactgt tcactcaact
     2701 ggttgagact gttttgcgct gccagccaaa ccggtgcacg ctgcatcaaa ctctgaatgc
     2761 gagactgcag ctgttcctgt tcctgccgca gtgccatccg ctcttcacgg gcgttagata
     2821 cgctatcgga aagagaggca atgcgtgctt ccagctcctg atgcagagct tccagttcat
     2881 caatatcaaa attcttgccc tgacgtttgc agaaatctgc cagcagacgc tcagcttctt
     2941 gctgctcacg cagacgctgt tccagttcgc ttaagcgcat ccgcaacggc tgaacctgct
     3001 ctgccaggtg acgctgatca accccttcgc gcaatagttc gcgagcgaca tcccacgcct
     3061 cgttacgcgc cagcgggccg ttgattgcca ccaccagctg ataagcctgc tcaaactggc
     3121 tgtgcgcggt ttgcgccatg ctcattttct gctcaagaga gagcattttt tcagtcgctt
     3181 ccagctcttt cgcctggaag gtttccagcc attcggcggc gctgtcggcg gttaagtccg
     3241 gcagatggca cagttctttg gcacgattaa gcgcagcaat cgcctggtta tactggatcg
     3301 cgcgcgtttg ctggacgtcc agcgcctgct ggtagtcagc aagctggctt ttcagctcgt
     3361 ccacttccag ttcggcagct tccgcgcgag cctcattctc ttcctggcgt tcgatggctt
     3421 ctgccaccac ttcattttgc tcttccagac ggatctgcag ctcatcgaga tccacttcgt
     3481 agcgttcgat cttctcctgc tgacgcaatg cggtttgcac caggttcagg tgatcgctgg
     3541 ccgcctgata atccgcttcc agatcacctt cggcaccgtt gtgctctgcc agctcacgcg
     3601 ccatatcgac gtgtttgtac tgctcagccg ccagttgctg acgcgaagta tgtagctcgc
     3661 gacgaaactc cagggctttg tcgagatgga cacggcgctc gttggcgtga cgcatgtagt
     3721 ccgccgccac gtagttggta gcttcgctga tcagatgctt aaacaggtca cggtccgact
     3781 gggtgacacg aatcgcttcc agcgtcatgc ggttttcacg cagcgccgct tccatgtcct
     3841 ggaacgcttt acgcacgccg ctgttttccg gcaacaggta gtcgcgcaga gaacgggtaa
     3901 tggcactgga gatcccgcca tacagcgaag cttcgatcag acgatagaat ttgctacggt
     3961 cagatgcgga gcgcagacga cgcgcgatga tgcccagatc gaacatcagc gagtggtaat
     4021 cggtaatgga gttgaactgt ttaaactgca cgccttccat cgcctcgagc ttgtctttca
     4081 gctcgttaag cggcagcacg cgcgcctggc gttcgttcag ggtttcggtc accagctgtg
     4141 tcggctgcac cgacatcggc agtccctgaa tggcaaatgg tttgatatcc actttacgat
     4201 cgcgtccggc aacctgttgc agacgcacac cgaccaccac gcgctggtgg cgcgagttaa
     4261 tggtgtcgag catcgaataa cagacacccg ctttcagctt accgtgcaga cctttatcgc
     4321 gcgaaccgct ggtggccccg gcttccgtag tgttacggaa atgcagcagg gtcaggtcgg
     4381 ggatcagcgc cgtaacgaac gccgccatcg tggtggattt acccgccccg ttaccgccgg
     4441 aaagcgtcgt gaccagctcg tcaaggtcaa aagttcgggc aaaaaagccg ttccagttaa
     4501 tcagcgtcag tgagcgaaat ttaccgcgtt caatcattat tcttcctctc cgctatctgg
     4561 ctgactctct tcggtttcat cgttgagttg cagatgattt tcaatcggca ttgcttcgcc
     4621 atcacgaatc aggcgacgct gtgcttcacg gggatcgtcg ccagcacgca catcggctcc
     4681 gaagcggaac accgattcgg taatgcggaa cttgctgctg tcgtggccca taaaccacac
     4741 catgcctaaa cgacgcagac ggttgagcga agaacgtact ttctcctgca acttctgacg
     4801 gtcaacgtct gaaccggttg aacggttgtt caccagtttc agcagtttcg cttcatcggc
     4861 cagggtgagc agttcgtcgt acagttcctg ctgggtgaaa atcccctcat tcgccagccg
     4921 ttccgggctg agatagagat aacagaggat tttcccgacc atcatatcca gttccgacaa
     4981 gacggaacgc gggataagag tggtggaacg tgggcgtaaa tagaagaacc cttccggtgc
     5041 gcgaataagc tcaacgttat aacgcgcgta aaactcttcc agatattcct gaaaatccat
     5101 caggaatgca tgattatcca gttcgtcgag gccaatatgg cggcctgaac gtaaggcgct
     5161 gtccagcgcc ggaaataacg gattcgccag cgcctgcgcc agcttaaccg gcatcacttg
     5221 ttcaatattt gtcgatgaca tgcgcctgta ccttggctcc gtaatcatta atcggctgcc
     5281 atttcgctgg cagtccggtg aaatctgctt gcgctacgcc aagacgtacc gcctgatcaa
     5341 taacaatacg cgcaacgtca aagtgacgtg cacgcggata ctgtgacaga tattcgcgta
     5401 ccaccagacc aagatccagc ggcacttgtc tggttttgta cacggcaagt tgttcttcga
     5461 tgatcgccgc cagctgttcg cggatctcgt taaactcttc gtattccaga tcctcaggaa
     5521 gttccccagt cacttcttca tcgcgcagtg ccatctcttc gtcacgcata tccagcagac
     5581 gatcggcatt ggcataagtt agcgcccacg gctcatcaaa ataggtttgt accgactgac
     5641 gtaaccgctg agcaaagacg cggtttttat ccatatcgat cgcggtacga ataaatttgt
     5701 gtacgtggcg gtcgtagcca atccacaagt cgatggattg ctggccccaa ctgataatac
     5761 gatcgagttt gctctgcaga tcgaacacca gacgatcgac gaaatgcaga tcgtcatggg
     5821 tcatcgtcgc atcctgaatg cgcaacagat tagcctgcaa tttgtcgcct gccgcttcca
     5881 gcgtatcctg caattcacgc agcgttccgg aagtttccga aagcaacaat tcacagctgg
     5941 aaatcgccgc ccgccagtct ttgttcagca actgggcgat atcgtccttc acctgctgct
     6001 gctgttcgtc catcagacgt tgcgtcaggt cgatactgtc gaaaatttct gctaccgaat
     6061 atttcagtgg cgcatagaca ttacggtgcc agtgaaattc atcaccgccc tcttcggcgg
     6121 catctgctgc gcgtttgagc tcacccgcca caatcgacaa ctgcatagaa agacgcagcg
     6181 tagaaaactc gcgctgacgg atgtagtagt cagtaatgcc gatgccgagc ggcgtcagac
     6241 ggtaaattgc gttcccttcc gcctgctcgc tggtaaagcg gttcagcaga cgttgacgca
     6301 ccatgtcgtt gatcgcgtta ttggcgcgca cgccgatggt ttcgctggtt tgctcaaacg
     6361 catcactcac atggcggaat gcatccacca gctcgccttc actcatctca ccatccagac
     6421 gctcgccgtt cagcgtggca accgccagca gaaaagagag tcggtctacc ggcagcgaga
     6481 tggagaagtc attttttctg gcccaggcaa ccagttcggg gactgtctgg gaaaattcac
     6541 tcatacttta tccttgctct gcggtttgcg cgcggtgaca tgaatataac gccctagggt
     6601 aatatacggt tcctgacggc aataacgcgt ttctaattca agtaatgctt catagcagtc
     6661 gcgctgctgg tacttctcgc gcagataatc atgaaacacg cgaacgcctg tcttacccat
     6721 aatttgccaa ccagcttctt ccagccacag ataaacctgc gccgggtcgc gtggataatc
     6781 tggcgaaagc gtccgttttt tctttttcgg cattcccgcc tgcacgtaat caaaattccc
     6841 ggcgaccatg ttatgcatca acaaaccatg cgcattgtag aacattaacg acaacactcc
     6901 gcctggacgc aacactgacc acagggtctg caatacactg cggggatcgg ccacccactc
     6961 gagcaccgca tggaacaata tcagatcaac gggcgtttcc aaatgcgaag caacatcctg
     7021 agcggcgcaa tgtataaatt gcatgttgtc gctcacacct tttgcttctg ccgcctgttt
     7081 tgcgcggtcg atcatctgcg cagaaagatc gcataaaata acctgatgcc cgcgctcggc
     7141 cattttgatt gcggtctgcc cttctccgcc gccagcatcc agcacacgca gtttttgcgg
     7201 gcccatttcc gccagcacgc gatcgagatc ctgccacaga atagcctgtc gaagctgccc
     7261 tttggtggtg ccgtaaatgt tacgggaaaa cttttccgca atatcatcaa aattgcgatc
     7321 ctgcattgct ttctccaccg gttggctaag acgttttctg ataaagacgc tattttgtca
     7381 cagccacggc gagaatgaac ccgtctggtg taatatcgct agcgatccct tgctatatgg
     7441 ttaaaaaagg aaccagaaag gatgcttttt acactgaaaa aagtgattgg caatatgttg
     7501 ctgccacttc cgctgatgtt gttaattatt ggcgctggcc tggcgctgct gtggtttagc
     7561 cgttttcaaa aaaccgggaa gatttttatt agcattgggt ggctggcact cttgttattg
     7621 agtttgcaac cggtagccga ccgcctgctg cgtcccatcg aaagcaccta tccgacatgg
     7681 aataattcgc aaaaagtgga ctatatcgtg gtgctcggag gtggttatac ctggaacccg
     7741 cagtgggcac cgagctctaa tttaatcaat aacagtctgc cacgcctgaa tgagggtatc
     7801 cgtttatggc gcgaaaatcc gggttcaaaa ctgatcttca cgggaggcgt agcaaaaacc
     7861 aatacggtga gtacagcgga agtaggtgcc agagttgcgc aatcgctggg cgtaccgcgc
     7921 gagcaaatta tcaccctgga tttgccaaaa gataccgaag aagaagctgc ggcagtgaaa
     7981 caggcgattg gtgacgcccc tttcctgctg gtgacctccg catcacatct gccgcgggcg
     8041 atgatctttt ttcagcagga agggttaaat ccgctaccag ccccggcgaa tcagttggcc
     8101 atcgactcgc cgcttaatcc gtgggaacgg gcaatccctt ctccggtctg gctgatgcat
     8161 agcgatcgtg ttggctatga aacgcttggc cgcatctggc agtggctgaa aggtccgtca
     8221 ggcgagccac ggcaagagtg attttgatgc cagatcgaag ttgcgccgac taaatcgtcc
     8281 ggtattaacc agttgcgcaa cttcatccca taacacatac aaccaacgcc gccagatgaa
     8341 cgactccgcc actggcgcgc gttgcaggta actccagagc aaatcttccg ccagagaatt
     8401 atccattagt cggaacagtt cgtattctcg cggtgcccaa agcattagtc ccggcccgac
     8461 catcgccagt aactgatcgc tgcgcgaatc tttcagcatg ctgcgtaaac agaagttacc
     8521 gtgaatcaac acacaattgt cgttaaagcc ttcaaataat gccgggagac attcgcgagt
     8581 gcgaaacagg atccgcttat cctgcatcgt cagaccggtg ttattgaact gattgagcgt
     8641 ggtccatagc acttcaacat gttgccggta ccatgagggc cagaaatttt cctgagtatt
     8701 gtcgaccgcg ccgacgcaac cgcgactgtc ctgacggtgc caggccagta aggcttcaac
     8761 gatttggtct ttgagctgtt cccagcgttc tggtgttcgg gctggtgcct ccaccgaaac
     8821 accacgcatc cgctccagta gcaggacatc agggccgggg tgttcttcat gcgtcatcac
     8881 accataaata gtcggcatac ggacagtccc acttcttgcc agcatggtgg ttttccatgc
     8941 cagttgtcgg gcttttccgg gcgtactaaa gcttcttgcc attaacggca ttgggtttcc
     9001 ctggctgtca tacaaagccc acaacgccgt atccgctttt tcattgacgc actcaatacg
     9061 actgagtttt tcgcccagta aatggcttaa ttcggcacgc agctgttcca ttcagattac
     9121 cctcataaaa gctactgctt tcataatgag cgccaatata aaagatgtca cctgaatgag
     9181 atcaattgtt gaaaagaaaa attgaggaaa atcgtaacgg tacgacactc ctcccaaaat
     9241 tggctgaagt gtcgtgaagt gaaattagcg catttcagcg cgaacgcgct caagatcttc
     9301 aggggtatcc acacctgtgc caggaacttc ctgagcaaca gcaacatgga ttttttcgcc
     9361 gtaccacaga acacgaagct gctctaacat ttcgatgtgt tctaacggac ttgcctgcca
     9421 gttgacgtaa cgacggataa agcctgcacg gtagccataa ataccaagat gacgcaggaa
     9481 gttatcgcca acggtttcaa ggccttctgc aaaacgatca cgatcccaag gaatggtggc
     9541 acgtgagaag tacagtgcat acccttcagc gtcgagaacc actttcaccg cattcgggtt
     9601 aaacgcttct tccgcattgt ggattggcac cgccagagtc gccataccca cctgacgctg
     9661 agcgaggtta tcagcaacct gacgaatgat tgtcgcaggg atcatcggtt catcaccctg
     9721 cacattaacg atcaccgtgt cgtcgctgaa tgcgcatttt tcgacaactt ccgccagacg
     9781 ttctgttcct gactgatgat cggcgcgcgt catacatact tcaccgccag cggcttcaac
     9841 ggcgcgggca acatcatcat gatcggttgc cacgatgatg cgctcggcac ctgattcacg
     9901 cgcgcgttca agaacatgaa caatcatggg tttgccgtta atatcaacca atggtttacc
     9961 gggcagacgc gtggacgcgt agcgcgcggg aataatgacc acaaaactca tgatttactc
    10021 tcatcagcag tcagcacgcg ggcttccgtt tccagcaaca ctggaatgcc atcgcggagc
    10081 gggaaagcca ggttatccag tttgcaaata agctcttgtt tttcctggtt ataccaaagt
    10141 tttccgttgc aaaccgggca ggcaatgatt tcaagcaaac gatgatccat agttcctcca
    10201 gatggatcgg gttatgaatg cataaatctt atcataatca tttatgcgac ggggtctatt
    10261 tcccagccag tgcggcaatc agtaaagaga ccttgcgggc agcgtcctaa tgtcacccgc
    10321 gttgcctgct gccagctcgc gaaatcagta atcgcctgac gtaacccttt ttgcagcatt
    10381 gtcgttggtt taatgccttc ctgtaaccac agagagataa cttcaaggat gcctgtctgg
    10441 cgatgcattt tggcatccat tcgcccaact aattgcccac gatgtaataa tggcagaaca
    10501 aaatagccat actggcgttt cggcgctggg gtatagcact ccagccggta gctaaaatca
    10561 aaaagctgct ctgcgcgttt gcgatcccag acaacaggat caaaaggcga aagtaccgcg
    10621 ctgtgcgttg cagtgagctt tccggccagc gcacgctcga gtagcggcag caaatcagca
    10681 tgcagccaaa gattgcccaa tttttcaaca tgcacagcaa tgatttgctg ctgttcagcc
    10741 ctcgcttcgc gccacgccgc cagtgccgga cgtttcagcc gatagtaatc tgccagccac
    10801 tgttcgcgga atattcccag gctacgcgca ctgttatcca gcatgatgat ttctgcttct
    10861 gtttgcgaaa cgagatcgcg ctcatcatcc cagtcaggca tgacacggtg ggttaaatca
    10921 taaacgcgct ggaagttgcg ccgttcaatc accatcacct ttccggcagt aaataaacct
    10981 tccagatgcc gtttatgcgg cttccattcc caccagccgc ttgcaccttt acgaggatgc
    11041 tcaaaatcgg ctgaacgtac cggcccttta tcatgaatat gctgaattaa ctgtgcaatt
    11101 tccgcctcat gttcctgcat ccaggcgtct ttgtatttcc agcccatttt ttcaggtgcc
    11161 agcatgcggt ggcgaataag acgaaagtcg ctacgcggca taaagcaggc ttcatgcgcc
    11221 cagtattcca ttaattcgcc acgcgccaga gactcatcca gccactgggc aggataattt
    11281 cccagacgac tgaaaagcac cagatatgga ctacgggcaa caatattgat ggtatcgatt
    11341 tgcagcaagg acatgcggga gatcgttgcc ggaatatcct ccaacgacgc tcgacggcgg
    11401 ggtttgttta acaggccttg tgcggcaagg tgaagattac gcgcatcagc aagggagagg
    11461 tgcggcagcg acattcatga ctccatcaat cgaacgctgc cgcggcgtaa ctagttgcca
    11521 gaagccagcg aggttagttg cgtaagcagt ttcgctggtt catcacctga aagctgtgcg
    11581 tctacaggca aataccacca attttcttct gcaaaggccc ggcatttcac cgcatctttt
    11641 tcagtcatta ccagcgtttg cccggcgctt accaacgcac tgacatcctc atggttcaaa
    11701 gactgatgat cggccagcgg tacacatttt tccggttgta cgccacacat cttcagcgtg
    11761 gcaaaaaagc gcggcggatg cccaatcccc gccatcgcca ctacatgttc aagctgagca
    11821 acgtcacaac gcgtaccggt acgtaaattc accgcctgac ccggcagcag atgcatgggg
    11881 atttcaccgc tgcgagggac accgccgttg acgattaccg catcaaccga ctttaagcgc
    11941 cccgctcgct cacgcattgg ccccgccggc aaccaccagc cattgccaaa gcgacgcaca
    12001 ccatcaataa cgacaatttc cacatcacgc gccagacggt aatgctgtaa accgtcgtcg
    12061 gttacgatga tctgcacatc agggtgttgc gccagaatgg cttttaccgc atcagaacga
    12121 acgggagaaa ccgcaacagg cgcatcagtg cgttgataaa tcaacacagg ttcatcaccc
    12181 gcctgtgctg tggtggtatc tgccgacaat aacagcggat aagattcagc cttaccacca
    12241 tatccccgcg atacgacccc cacgcgaata ccgcgctgtt gcaactgttc caccagccag
    12301 acaacgaccg gggtttttcc gttgccgcct gcggtgagat taccaaccac gacaaccggt
    12361 acgggggcac gccaggcgcg cttcagtttt agtttatagc aaagacggat cgcgccactc
    12421 accaggccat acaaccagga gagtggcagc aatagccgcc acaaagggga ttcaccagac
    12481 cagatttttt cgatcattgg ccaaactgca ttttgtgaag ttgcgcgtaa acgccgcggt
    12541 gctcaagcaa atcgttatgc gtaccgcgtt ccacaatgac accatcctcg acgaccacga
    12601 tttcgtctgc cttttcaatg gtagacaagc ggtgggcaat caccagagag gtacggtttt
    12661 tctgcaactc atccagtgcc gcctgaatcg cacgttcgga ttcggtatcc agagccgagg
    12721 tagcttcatc cagaatcaga atcgggctat cacgcagcaa ggctcgagcg atagcaatac
    12781 gctgacgctg accgccagag agcagcacgc cgttttcacc aatcactgta tcgagaccgt
    12841 tatccatctt attgatgaag tccatggcgt aggccatccg cgccgcttct tcaatttgct
    12901 cacggctgta ctgttcagtc cgtgcgtaag caatgttgtt agcaaccgta tcgttaaaca
    12961 gatggacatt ctgcgacacc agagcaacct ggttacgtaa cgacgccagg gtatactcgc
    13021 gcagatcgtg accatccatc aggatttcgc cttcatcaat atcgtaaaaa cgcgtgatca
    13081 ggctggcgat ggttgattta cctgaaccag agcgtccaac cagagcaacc gtcttccctg
    13141 ccggaatttt caggttgatg ttacgcaatg cgggtacgtc acgtcccgga taagtaaagg
    13201 tgacattgcg gaattccacg tcgccagtcg cacgctcgat cacgcgctta ccttcgtctt
    13261 tctcctgctc actgtccaga atggtaaaca gcgtctgaca agccgccata ccgcgctgga
    13321 actgggcgtt aacgttggtc agcgatttca gcggacgcat cagtgcaatc attgaagaga
    13381 aaacaacggt aatcgtaccg gcagtcaggt tatccataac acttgggaag ctcgccgcat
    13441 acagaacaaa cgccagcgcc aaagaggcga tcagctgaat gatcggatca gagatggaag
    13501 aggctgaaac cattttcatc ccctgaagac gcattcggtt actgacttta tcgaagcgtt
    13561 tcgtttccac ttcctgacca ccgaaaatca atacttcttt gtggcccttc agcatttgtt
    13621 ctgcactggt ggtcacttgc cccatggtgt tctgcatgtt tttactgatg ttgcgaaaac
    13681 gcttcgatac tacgcgaatc gcaatcgaaa caatcggtgc cagcacaatc aaaatgatcg
    13741 acagttgcca actgtaatag aacatcatga tgaacaggcc gatgatcgac gcaccttcac
    13801 gcacaacagt aatcagtgcg ccggaagaag aagaagcaac ctgttcggaa tcgtaggtga
    13861 tacgtgacaa cagcgtaccc gttgactgtt tgtcaaagaa tgaaactggc attcccatca
    13921 tgtgaccaaa caggcggcga cgcatggtca ttaccacctt tcctgatacc caggagatac
    13981 agtagctgga gacatagctg gtgataccac gtaaaatcat cagtccgatc accaccagcg
    14041 gcatccacac cagcacggag cgatctgttt taccaaagcc atcatcaaga agtggcttaa
    14101 ggagcgataa catgaaggta tcgctggctg cgttgaggat taacgctacg cccgccacga
    14161 tcagacccgc tttgaaaggc gcaatggttg gccacagtcg gcggaatgtc tgccacgtag
    14221 agagatcttt gtcgttatgc attcaaaaaa ccagcatttg ttgaaatagc cgcatattct
    14281 acccgttatc cactggcacg ccaaaccact gatgatacca acgaggtaaa atttgctctc
    14341 tgaggctgct aatccgccag ccttgtgctg aaaaattgac cgtcacttgc ccctgatgtg
    14401 gagtatcaag ccattgatat cctttctgtt gatagcgatg cttaactttg ttagagggca
    14461 atcgccatgc gttatagcgc gatgccgatg cgagtgccac ttttccattc actcgctgaa
    14521 ttaatggcaa cgatgatgag gtattactgc catggtgagg tacctgaagc aatgttgcct
    14581 gcacttgctg ccagtaacgg cttagcatct tttgttcagc tggggcttca atatcaccgg
    14641 ttagaagaat gctattcgtc ccgtcatcaa ccttaaccac acaggaatgg ttatttccct
    14701 tatcgttgct accttgtaaa ggccagtgcg cgctgaaacg caatccttgc cactgccacg
    14761 cttcgccacg cacacagggc tgatgatgtt cccagtttaa cggacttctg atccataaca
    14821 tcggccatat atgcaatatt gaatccagcc ctccccggtg atccagatgt tcatggctca
    14881 gaataacgcc ttccggttca agattatgcc agtggagcca ggggataatc agttgttgcc
    14941 cactatcccc ttcaggccag tcctgtgtca tagagaatcg ctttgccgtt tctggctatc
    15001 accattgcca gcccttgccc gacatcaagc atgtacagct gccactcgtc aggtcgaggt
    15061 ttttgccaca gcggccagca catcagcaag cctccagcca cacacattgc tggcaacgtt
    15121 cgccaggcgt ttaatcgcca taccaccagt aagaaccatg gggaaaatga tagccattgc
    15181 caacattcaa caatgttgat ccacccttcc ggcaaactct ttaaccccca gaaaagtaaa
    15241 gccaaagacc ggtcggcaag aaaccataac ccctgttcca ggattaacgg cccgctaaaa
    15301 tgcacaacca tcgcggcgag gatcaacgga accgtgataa atgtcaccaa gggaattgca
    15361 aatagatttg caataaacga ggtcagacta atgccatgaa atatgacgat ttgcatgggc
    15421 ataagcagga gtgtgattcc cagttgcaga tggatgaggg aaacaactgc acgcaatacc
    15481 ggcggcagtt gccactcagg acagggaaac cactgatacc aaaaaatcag tgccgcgacc
    15541 gcagcggcag agagccataa actttgcgag agaatggcaa caggatccat cagcaaaatt
    15601 gccgccagac aacatatcca tacatcccag ccactccact gtcgcccact taacttaagc
    15661 attccccacg tagcaagcgc caccacggta cgcaatgcag gaggttgcat tcccgtcaac
    15721 caggcataaa aagcagcaca gcagattccg ccaattaatg gtatttgcca gtggatccag
    15781 cgcccaggca gaaaaatttg tccactgcga atgagtccgg cagccagcaa cgccgcaaaa
    15841 gcgatgtgca atcccgatat cgccattaaa tgcgccgttc cagtatcgcg catgatattt
    15901 ttgatttctt tggggacgga taaccgttcc cccataccta aaccaagaat aaccgcattc
    15961 cacggatagg gttgcagcgt tgtttgtagt gacgccagat actgtgcacg caggctacaa
    16021 ttcggttcaa tgacacttgc ctgcagaaaa cggccggtga gcggctgatg ctgggcaatg
    16081 gcataacgct ggctatcaaa gccgccatca ttaagttggc catgaactgc acgaactttg
    16141 agtttcattg accattgttg tccagcacaa accgcttgcg gaagttattc gccatacagc
    16201 acaaggcctg gcgcagggaa tatacgtcga ccttgtagat aagtaatttg accgtaatga
    16261 gtggtcatgc cgtcagttgc agtgatctca acaattgcat cctgcgtcgc gccagttaag
    16321 gtttctcctg cccacaaaat ttgctttgct gataatatgc cccaaacgaa aaacagcaac
    16381 gtcagcgcga tataacggac ggttttaaca gggataaatg ccagtacgca ggcgaagaga
    16441 gtcagaaacg caagggttaa tgtcctaggc aattggggca aaatcagcaa cggaaaaatt
    16501 ccgcaaatta tgcatacgcc gactgtcgtt attttcatgc acccctccct gtttatggaa
    16561 agtgtgcaac tttgttgagc ggttgtctgg tgggaatttt gcgttccccg aggcacattc
    16621 agaagaattt aacgaaaggg tgaaaactgc tcaaaactct gcgaggcagc ttccagttcc
    16681 tgattagaga attgaaaaaa gcacccgaca ggtgcttttc tcgcgttcaa gtttgagtaa
    16741 aaacttaacc gtaaatattg gcgcgatcgc gcagttcttt accaggttta aagtgaggaa
    16801 cgtattttcc ttccagttct actttatcgc cagtcttcgg attacgtccg gtacgtggtg
    16861 cgcggtagtg caaagagaaa ctgccgaaac cgcggatttc aatacgctcg ccctgcgcaa
    16921 gagtcgaggc catatgctcc agcatctctt ttactgcatc ttcaaccgtc ttggcgggaa
    16981 tgtgcgattg ctgggtggca agtctttcta tcaattctga cttggtcatg attcctccgg
    17041 ttccttaaag gcaaattaag cggctgctgt tgctgcattg attaagggcg gccgtagccg
    17101 cccttagtgc ttgattacag gacgaaacct gcaatctgtc aagtaaactc aacaaacttc
    17161 ggaataaaaa tcccgaagag tcagagaatt actcgccttt agctgctttg aaagcttcag
    17221 ccattgcgtt gttggagaag tttgcatctt cctgtttgtt aacagttgcg attgcatctt
    17281 tctcgtcagc ttcgtctttc gcacgaacag acaggctgat tgcgcggttt ttacgatcaa
    17341 cgccggtgaa tttagcttca acttcgtcgc caacgctcag aaccagggta gcgtcttcaa
    17401 cgcggtcacg ggatgcttca gaagcacgca ggtaaccttc aacgccgtca gccagttcta
    17461 cggttgcgcc tttagcgtca actgcagtta ctttaccggt tacgatagcg cctttcttgt
    17521 tcagagcaac ccagttgttg aacggatctt ctgcgagctg tttaacgccc agggagatac
    17581 gttcacgttc tgcgtcaacc tgcagaacaa ctgcagcgat ttcgtcgcct tttttgtatt
    17641 cacgaactgc ttcttcgcct gcaacgttcc aggagatgtc agacaggtga accaggccgt
    17701 cgatgccgcc gtccaggccg atgaagatac cgaagtcagt gatagacttg attttacctt
    17761 caacacggtc gcccttgttg tgggtttccg cgaactgctg ccacgggtta gctttgcact
    17821 gtttcagacc cagggagata cgacgacgtt cttcgtcgat atccagaacc ataacttcca
    17881 ctacatcgcc aacgttaaca actttggacg ggtggatgtt tttgttggtc caatccattt
    17941 cggaaacgtg taccagacct tcaacgcctt cttcgatttc aacgaagcag ccgtagtcag
    18001 tcaggttggt cacgcgacca gtcagtttgg taccttccgg ataacgttta gcgatagcta
    18061 cccacggatc ttcgcccagc tgtttcaggc ccagggatac acgggtacgt tcgcggtcga
    18121 acttcagcac tttaacagtg atttcgtcgc ccacgttgac gatttcgctc ggatgcttaa
    18181 cgcgtttcca ggccatgtca gtgatgtgca gcaggccgtc aacgccgccc agatcaacga
    18241 atgcaccgta gtcagtgagg ttcttaacga tacctttaac ttccatgcct tcctgcaggt
    18301 tttccagcag ctgatcgcgc tctgcgctgt tttcggattc gataacggca cgacgagaaa
    18361 caacaacgtt gttgcgcttc tgatccagct tgattacttt aaattcaagc tctttgcctt
    18421 ccaggtgcag agtgtcacgc accggacgaa cgtctaccag agaacctggc aggaacgcac
    18481 gaataccgtt cagctcaaca gtgaagccgc ccttaacttt gccgttgata acaccggtaa
    18541 cagtttcagc atcttcgtaa gctttttcca gcgtgatcca ggcttcgtga cgtttagctt
    18601 tctcacggga cagcagagtt tcaccgaagc cgtcttctac tgcgtccaga gcaacgtcaa
    18661 cttcgtcacc tacctggatt tccagctcgc cctgggcgtt tttgaactgc tcagccggga
    18721 tggcggactc agatttcaga ccagcgtcaa ccagtactac gtctttgtcg atagcaacaa
    18781 caacgccacg aacgatagaa cccgggcggg tttcgatttc ttttaaggac tcttcaaaga
    18841 gttgagcaaa agattcagtc atgtttaatc ttcaggttta tatttaacgt ccacctggct
    18901 ccatgccgga tggggttgtt taacataccc gctgtcattc cattgcaacg ggggtactgc
    18961 aaattcggtc gcttatgcga gagccaattt ctggcgcgcg tattgtagcg ctttttcaat
    19021 caattgctca atgcttaagg tggtggaatc caacactaaa gcatcggctg ccggaaccag
    19081 tggcgctacc gctcggttac gatcgcggtc gtcgcgttct ttgatctcgg ccaaaaggcg
    19141 ctcgaagtta acactaaagc ccttctcctg caactgtagc atgcggcgat gcgcacgttc
    19201 ttccgaggag gcgtcaagga aaattttcac tggtgcatca gggaatacca ccgttcccat
    19261 gtcgcggcca tcggcaatca gacctggtaa ttcgcgaaac gcgcgttggc gacgcaataa
    19321 tgcttcacga acgcgtggga atgccgcgac ttgtgaagcc gcattcgcca cttcctgagt
    19381 acgaatttcg ccgctgacat cttccccttc gaggatcact tccagattgc cattggtcga
    19441 cacaaaacgt acatccagat gggatgccag cggtaccagc gcatcttccg acgcaacatc
    19501 aacatggtga tgtaatgccg ccaatgccag tacgcgataa attgcacccg agtccagcag
    19561 atgccattgc aacgcttccg ccatagcctt acacaaggtg cctttccctg caccgcttgg
    19621 gccatcaatg gtaataaccg gggcaattgc cgtcatcttt atctccttaa aacaggcgta
    19681 ccgttaacat aaccgcgcgc attatacgcg ccgacgccac aaatcgttaa cgttgtgtgc
    19741 aaaaaccagt tttgtctgta ttcagatggg gatttattgc gcaattatag cgggctggca
    19801 gaagaccagc ccgaaagatg acaatgattt acttaccaga ggcgatacga tcacggatgt
    19861 gttgcgcacg ctctgttgat ggtgggtgag agtcaaacat ggactgggtg cgaccgccat
    19921 ccaggctagc cagtttttca aagctgccaa ccagcccctg ggtgctgatg ccacgtttct
    19981 tcaacagatc aaaagagaaa tcatctgctt cggactcttt atcacgggag tatttagcat
    20041 taatagcgcc ttctgcgata tcacccaatt gtgagcggga aagctgggaa gccacaccgc
    20101 tggtagctga aatggcatcg cgtgcggcaa cgatcgcata agaagctttc atttcagcca
    20161 gcgagtgacc caacgcgacg tggcccagtt catggcccag aacaccttca atttcgttgt
    20221 cattcatcat gtccatcagg ccactgtaga cacgaacaca gccgttcgcc atcgcccatg
    20281 cgttgacgtc gctggtcata taaaccttat agttgaccgg cgtgccatta atattgttac
    20341 ccaatgcttt ggcgattttt gccagacgtt taccgtactt gctggattta cttgcgactt
    20401 gattgccgct gtccatttcg gcacagccct gattggcaat cgcttttgca tcggcatcgc
    20461 ttaatgttgc tgctttatag gcatttaaac cggagctgat agccatattg gtatcaatac
    20521 cgtgggtatt ttgacaccct gtcagtaatg ctgcagaggt cgcaatcgcc agcagtaatt
    20581 tagtattctt catgtgaatc atccgttgat taatcatata taaatattat gtatcaacaa
    20641 cttacattga caagcgacgc taccattaat caaaatgact cacaaggtcc gaaaaaaaac
    20701 gccgacaatg tgc
//
LOCUS       DAZPCS010000056        20691 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZPCS010000056 DAZPCS010000000
VERSION     DAZPCS010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20691
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.056"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            81..350
                     /locus_tag="ACHUQ7_002046"
     CDS             81..350
                     /locus_tag="ACHUQ7_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HFR1135461.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            344..709
                     /locus_tag="ACHUQ7_002047"
     CDS             344..709
                     /locus_tag="ACHUQ7_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFR1135462.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VAMCCPISTGANAARSTGVTVNVLPRDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(710..1498)
                     /gene="bioH"
                     /locus_tag="ACHUQ7_002048"
     CDS             complement(710..1498)
                     /gene="bioH"
                     /locus_tag="ACHUQ7_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFR1135463.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            1536..2219
                     /gene="gntX"
                     /locus_tag="ACHUQ7_002049"
     CDS             1536..2219
                     /gene="gntX"
                     /locus_tag="ACHUQ7_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFR1135464.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            2278..2853
                     /gene="nfuA"
                     /locus_tag="ACHUQ7_002050"
     CDS             2278..2853
                     /gene="nfuA"
                     /locus_tag="ACHUQ7_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFR1135465.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            3214..4530
                     /gene="gntT"
                     /locus_tag="ACHUQ7_002051"
     CDS             3214..4530
                     /gene="gntT"
                     /locus_tag="ACHUQ7_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFR1135466.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(4575..6659)
                     /gene="malQ"
                     /locus_tag="ACHUQ7_002052"
     CDS             complement(4575..6659)
                     /gene="malQ"
                     /locus_tag="ACHUQ7_002052"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFR1135467.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(6669..9062)
                     /gene="malP"
                     /locus_tag="ACHUQ7_002053"
     CDS             complement(6669..9062)
                     /gene="malP"
                     /locus_tag="ACHUQ7_002053"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFR1135468.1"
                     /translation="MSQPIFNDKQFQEVLSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLTDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VCLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENLQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            9674..12379
                     /gene="malT"
                     /locus_tag="ACHUQ7_002054"
                     /pseudo
     CDS             9674..12379
                     /gene="malT"
                     /locus_tag="ACHUQ7_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
     gene            complement(12422..13438)
                     /gene="rtcA"
                     /locus_tag="ACHUQ7_002055"
     CDS             complement(12422..13438)
                     /gene="rtcA"
                     /locus_tag="ACHUQ7_002055"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFR1135469.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFSTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGIFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(13442..14668)
                     /gene="rtcB"
                     /locus_tag="ACHUQ7_002056"
     CDS             complement(13442..14668)
                     /gene="rtcB"
                     /locus_tag="ACHUQ7_002056"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFR1135470.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQLFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            14849..16447
                     /gene="rtcR"
                     /locus_tag="ACHUQ7_002057"
     CDS             14849..16447
                     /gene="rtcR"
                     /locus_tag="ACHUQ7_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HFR1135471.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCIYQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(16429..17187)
                     /locus_tag="ACHUQ7_002058"
     CDS             complement(16429..17187)
                     /locus_tag="ACHUQ7_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFR1135472.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(17204..18034)
                     /gene="glpG"
                     /locus_tag="ACHUQ7_002059"
     CDS             complement(17204..18034)
                     /gene="glpG"
                     /locus_tag="ACHUQ7_002059"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFR1135473.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYMQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(18079..18405)
                     /gene="glpE"
                     /locus_tag="ACHUQ7_002060"
     CDS             complement(18079..18405)
                     /gene="glpE"
                     /locus_tag="ACHUQ7_002060"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFR1135474.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            18595..20100
                     /gene="glpD"
                     /locus_tag="ACHUQ7_002061"
     CDS             18595..20100
                     /gene="glpD"
                     /locus_tag="ACHUQ7_002061"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFR1135475.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANALMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYTRTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(20306..20587)
                     /locus_tag="ACHUQ7_002062"
     CDS             complement(20306..20587)
                     /locus_tag="ACHUQ7_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1135476.1"
                     /translation="MQNRKWILTSLVMTFFGIPILTQFFAAVIAMLGAGLAAIPEVCN
                     LLFTPTIYLLLNIFMLTLGALMLFFSGRVGNVPEFCYVGYDGVGFAVIS"
BASE COUNT         4888 a         5692 c         5217 g         4894 t
ORIGIN      
        1 gtcagtagat actctaccct gcttaagtgc tactttgtgc tatcttgcat gtaacacaaa
       61 gtagcactta gggaactgat atgcagacag tgaaattacg gcaacaagga ggagccatga
      121 tcgttactat cccacgagat cttgccatcg atcttggctg gtcaccgggc acagagctta
      181 ccgtggagaa aaaaggcgat agcgttaatt tgcgcgccac agagcacaaa ccgcgtgggc
      241 gtcttaccgt cgcgcagttg ttgagccaaa tcgatgaaag cgaaattacg gagctaaatc
      301 agtccacaga aggatgggct gaagggaaaa aaggtaatga agcatggtaa aggcacggac
      361 gccacatcgt ggtgagatct ggtattttaa ccctgatccg gttgccgggc atgaacttca
      421 ggggccacat tattgcattg tggtaacgga caaaaaactc aacaatgttt taaaagttgc
      481 tatgtgctgc ccgatttcaa caggggcaaa tgcagcacgt tccacagggg tgacggtgaa
      541 cgtcctcccc cgtgatacgc aaaccggtaa cctgcatggc gttgtacttt gtcaccagct
      601 aaaagccgtc gatcttattg cccgtggcgc taaatttcat accgttgccg atgaaaaatt
      661 gattagtgaa gttatcagta aactggtgaa tttaatcgac ccacaataat tagctctcac
      721 ttaccaccaa caccctctgc ttcaacgcca ccagcaggtg acaaaactcg accggatgcg
      781 aaataaacgg cgcatgggcc gctttggcga agatatatga ttcgctgtga ggccaaagtt
      841 tatccagcat cggcaccact ttgcgcggca ccagaccgtc gagatagcca tacaatcgca
      901 aaaacggcat ggacacgttt tgcagcggct gacggagatc gaccgttttc aggatttcca
      961 gcccgccatt aagcacgtca acctccggca tcggtaacgc cagaacggtt ttcttcaacg
     1021 cccgcgcatc ctggcgcgcc gtttcagtcc ccatggtttg taacgccagg aaccgctcca
     1081 ctgtacgctg aaaatcatca ctgagttgct gctgaaatcc cgccagcacg tccggtttta
     1141 tccccggcca ctcgtcacga gcactaaaac aaggtgacga cgccacggtg accagcgcct
     1201 gaacacgctc gggatgggtt aacgcaatct ggcttgccac cagcccgccc agactccagc
     1261 ctaaccaaat ggctttatca ggtgcctgtt gcagcacggc ttcggccata tcagcaagtg
     1321 acagcgcacc aaatccccgg ctacgcccga agccgggcag gtcaacaagg tgcagcgtaa
     1381 aatgcgagct aagttcctcg tcaatgcaac gccacacttc ggcattcagt ccccatccgt
     1441 gcagcagcac aagatgaaca ttcccctgac ctttggtctg ccaccagatg ttattcatcc
     1501 gctattgttc tcttttgact tacaaggatg aacatatgct aacagtaccg ggattatgct
     1561 ggctatgccg aatgccactg gcgttaggtc attgggggat ttgttcggtc tgttcacgcg
     1621 ccacccgcac agataaaacg ttatgcccac aatgtggatt acccgccaca cactcccatc
     1681 ttccctgcgg tcgctgcctg caaaaaccgc cgccctggca aagactggtc acggttgccg
     1741 actatgcgcc gccgttaagt ccgcttatcc accagcttaa attttcccgg cgcagtgaaa
     1801 tcgccagcgc cctgtcacgt ctgttactat tggaagtctt acacgctcgt cgcaccaccg
     1861 ggttgcaatt gccggatcgc atcatcagcg ttccgttatg gcagcggcgt cactggcgtc
     1921 ggggatttaa tcagagcgat ttgctgtgtc agccgttatc acgctggttg cactgccaat
     1981 gggatagcga agccgtcaca cgtacacggg ccactgcgac ccagcatttt ctcagtgccc
     2041 ggctgcgcaa gcgcaacctg aaaaatgcct ttcgtcttga attgcccgtg caaggtcgcc
     2101 atatggtgat tgtggatgat gtcgttacca ccggaagtac cgtcgcagag attgcgcagt
     2161 tgcttttacg caatggtgcg gcgactgtcc aggtctggtg cctttgtcga accttgtaga
     2221 gcctcgatga tgggcgtatt ataaccaact aaaatagtca actattaggc cattactatg
     2281 atccgtattt ccgatgctgc acaagcgcac tttgccaaac tgctggcaaa tcaggaagaa
     2341 gggacacaaa tccgcgtatt tgtgattaac cctggcacgc ctaacgctga atgtggtgtt
     2401 tcttattgtc cgccggatgc cgtggaagcc accgacactg ccctgaaatt tgacctgctg
     2461 accgcgtatg ttgatgagtt aagcgcacca tacctggaag atgcagagat cgactttgtt
     2521 accgaccagt tgggttctca gttaacgctg aaagccccga acgccaaaat gcgtaaagtg
     2581 gcagacgatg caccgctgat ggagcgcgtg gagtatatgc tgcagtcgca gatcaaccca
     2641 cagcttgctg gtcacggtgg tcgcgtttcg ctgatggaaa tcaccgaaga cggttacgcc
     2701 attctgcaat ttggcggcgg ctgtaacggt tgttccatgg tcgatgtgac gctgaaagaa
     2761 gggatcgaga agcagctgct gaacgaattc ccggagctga aaggtgtgcg cgatctcacc
     2821 gaacaccagc gcggcgaaca ctcctactac taagttatcc cctcttttgt agattgcccg
     2881 atgcgacgct aaagcgtcat ttcgggccta cgttgaacat gtgccagacg ttggcacagc
     2941 attgctttgg caaatccctc ccttcgtttc cccgcgataa tatgaccaac ctctcataat
     3001 ttaaatttac cccgctctgg tgattctcaa acgccaaatg ttacccgtat cattcacatg
     3061 ggtaccaaac atactcctga catctgacta caataattag ttttagtggg tatcagtcgt
     3121 ggtgccgcaa tatctctgtt cccgattggg ataattagag tttgtcgtca gaaaattgac
     3181 gttacccata acaaatgaaa ggccaggtaa atcatgccat tagtcattgt tgctatcggt
     3241 gtaatcttgt tgttgctcct gatgatccgc ttcaaaatga acggcttcat cgctctcgtc
     3301 ctcgtggcgc ttgctgttgg attaatgcaa ggaatgccgc tggataaagt tattggctcc
     3361 atcaaagccg gtgtcggcgg gacgctcggt agccttgccc tgatcatggg ttttggcgca
     3421 atgctgggca aaatgctggc agactgcggt ggcgcacaac gtatcgccac cacgctgatt
     3481 gccaaatttg gtaaaaagca catccagtgg gcggtggtgt taaccggttt taccgttggt
     3541 tttgccctgt tctatgaagt gggcttcgtg ctgatgctgc cgctggtgtt taccatcgcg
     3601 gcttccgcga atattccatt gctgtatgtt ggtgtaccaa tggcggctgc actgtctgtg
     3661 acccacggct tcctgccacc gcatccgggc ccgactgcga ttgccaccat tttcaatgcc
     3721 gatatgggta aaaccctgct gtacggtact attctggcaa tcccgaccgt gattctcgcc
     3781 ggtccggttt acgctcgcgt gttgaaaggt atcgataagc caatcccgga aggtctctac
     3841 agcgcgaaaa ccttcagcga agaagagatg ccgagctttg gcgtcagcgt ctggacctct
     3901 ctggtgccgg ttgtgctgat ggcgatgcgt gcgattgccg aaatgatcct gccgaaaggt
     3961 cacgctttcc tgccggtagc ggagttcctc ggtgacccgg taatggcaac gctgattgcc
     4021 gtgctgattg cgatgttcac ctttggtctg aaccgtggtc gttcaatgga tcagattaac
     4081 gacacgctgg tttcttccat caaaatcatt gcgatgatgc tgttgatcat cggtggtggc
     4141 ggtgcgttca agcaggtgct ggtagacagc ggcgtggaca aatacattgc ttccatgatg
     4201 cacgaaacca acatttctcc gctgctgatg gcctggtcga ttgctgccgt actgcgtatc
     4261 gcgctgggtt ccgcaaccgt tgcggcaatc actgcgggtg gtatcgcggc accgctgatt
     4321 gcaacgacgg gtgttagccc agagctgatg gttattgcgg tgggttccgg tagtgtgatt
     4381 ttctctcatg tgaacgatcc gggcttctgg ctgttcaaag agtactttaa cctgactatc
     4441 ggcgagacca tcaaatcctg gtcgatgctg gaaaccatta tctcggtgtg cggtctggta
     4501 ggctgtctgc tgctgaatat ggtgatttga taacactgac tgccggatgc atcgcatccg
     4561 acatcaaccg cactctactt cttcttcgct gcagctctgc gccgtctgtc caaatccttc
     4621 agcaacttgt tcacgccatc atcggcaaac atcgactcaa gcgttgcgga aagcttgcgt
     4681 cgccagtttt tatactggta actggtgcca ggaatattca ccggttcggc catatccagc
     4741 cagtcttccg gctgtagtcc taacagagca ctgttactgt cggcaatgta gcgctgcaaa
     4801 ccacggttca gcgtcggcgt catcgacatc aacgatgcct tatgcccggc acgttttggc
     4861 agacaaccat atttatgcag tgcatccagc agcccttgct tcgccagttc gcgatcctga
     4921 tacagaccgc gcagtaccac ttcatccgga tacagcccca gggttttgcc cagcgttaga
     4981 tccccgctct cccagtaacc acgcagcgtt ggcaggtcat gtgtcgccgc aaccgccatc
     5041 gactgctccg gatacgcttt cggtgcacgg aacgtcttct cgtggtcgtt ttcgaaatag
     5101 agcactttgt aagagtacac accgctgctg cgcagcttac caacaatctc taccggtacg
     5161 gtaccgagat cttcaccaat caccatacag cgatgacgtt tactttcgag tgccagaatc
     5221 gagagcagat catccaccgg atagtgaaca tacgcgccct gatctgccgt ctcgccatac
     5281 ggtatccacc acaaacgcag catcgacatc acatggtcaa ttcgtaatgc gccgcagttt
     5341 tgcatattgg cacgcaacag ctcgataaac ggttcatagg cacgcgcggt gatgatatgc
     5401 gggtccattg gcggtaatcc ccagttctgc cccaacgggc cgaggatatc cggcggcgcg
     5461 ccaaccgatg ctttcaggca atatagttca cggtcacacc aggtttccgc cccaccttcc
     5521 gctacgccaa ccgccagatc acgatacaag ccaatcggca tttcatagcc ctggcttatc
     5581 tcccagcagg cggcaaactg gctgtaagcc agccactgca accagagata aaaatcgacg
     5641 tcatcacgat gttcttcgca gaactgacgc acttctggtg aatccacgtt cttatacatc
     5701 tctggccatg caggccagcc ccagcgcatt tcgtcctctt tcacttgctg ggcatgtagc
     5761 gcatcaaagg cagcctgcca gaacaggctg tcgccctgct ctgcaacaaa ctggcgaaac
     5821 gcggccatct gctcatcatc acgttgcgcg aaacctttcc acgccattcg taatgctgtc
     5881 atttttaggg cggtaaccgt ggagtaatcg acccagtcgg catcgcgcgc ctgttgcagc
     5941 gtctgttgcg tggtcggcaa ctgccaccag gcctgagcct cttcgctaag atggaaatct
     6001 tcaacggcgt taacgtcgat ataaatcaca ttcagccaac ggcgagaaga cgggctgtat
     6061 gggctggcgc tctccggatt tgccggatag agcgcatgaa tcgggttcag gccaatgaac
     6121 gacccgccac gttttgccac atccaccagc atcgctttga gatcgccaaa atccccaata
     6181 ccccagtttt tttccgatcg cagcgtataa agctgaacgc aggcacccca cagcttttgt
     6241 ttattcagca aagcctgcgg ttcgtaacaa cgtttcgggg cgacaatcac ccggcaatgc
     6301 gcgcgctggt cgtcctgggt gagtgttagc gtgtgataac cttccggcag cttcgtcggt
     6361 agattgaacg ctttgccccc cgttacatgg cctttgtact gcgttccttc ttcggtggtc
     6421 agctgccagc tatattcgcc gctgccctcc accaccatcg gcattttttt gccgctggta
     6481 taaaccatga cattcgggac tggcgttacc gccactttcg tggcggtacg ttgatgcatc
     6541 gcgtcaagca aacgccgttt ggtttcggcg ctaatcgact gcggtttacc gtgggcattg
     6601 atgtaattgg ggctaatccc cgccgccagc gcggcattat ccagacgttt gctttccatc
     6661 gagcttcctt agcgttttgc ctgccagata cgagcctgat aatcgcgaat agagcgatcc
     6721 gagctaaaca taccgcagcg ggcggtattg aggatcgccg cgcgagtcca ggcctcctgg
     6781 tcgcggtaca gcacatccac ctgcttttgt gcctcaacat aggctgcgaa atccgccatc
     6841 accagatacg gatcgccgcc ctgtttgccg atactgtgca gcatctggtc gaaggcatgc
     6901 ttatcgccgt cgctgtattt accgctttcc agctctttca atactgcgtc cagcacctta
     6961 tctttcttcc gccatttcac cgggtcgtag cctttggcca gaatagcctt cacttgttcc
     7021 acggtatgac caaaaataaa gatattttct tcaccgactt tctcggcgat ttcaacgttc
     7081 gcaccatcca gcgtaccgac agtaagcgca ccattgagcg ccagtttcat attgccggta
     7141 ccggaagctt ctttacctgc agtcgaaatt tgttcggaga tatccgccgc cgggatcagt
     7201 ttttccgccg ccgaaacgca ataatccggc aggaacacca ccttcaactt atcgccaacc
     7261 agtggatcgt tgttgatcac gtcagccact ttgttgatcg caaagataat attcttcgcc
     7321 aggtagtagc ccggtgccgc tttcgcgccg aagaggaaga cgcgcggtac gcgatcagcc
     7381 tgcaggtttt cacgaatttc tttgtacaac gccagaatat gcagcagatt caggtgctgg
     7441 cgtttgtact catgcagacg tttgatctga atatcgaaaa tcgcctgtgg attgatctca
     7501 ataccggtac gaactttcac aaactccgcc agacagactt tattcgcctg cttgatctcg
     7561 cgatattgct gacggaattt cgcatcatca gcgaattttt ccagattgat cagctgatcg
     7621 agatcgttag cccactcttt ttgcagtgat ttatccaaca gagccgccag tgcagggttg
     7681 cactgtttga tccagcgacg tggggtaata ccgttggtaa cgttatggaa tttgttcggc
     7741 catagctggt gatattccgg gaacagatct ttcaccacca gatccgagtg cagcgccgca
     7801 acaccgttca ccgcgaaacc gccaaccaca cacaggttcg ccatatgcac ttgtttatcg
     7861 tgcaccaccg ccagtttggc ccacactttt tcatcgcccg gccaggtttt ctccaccagc
     7921 gttttaaagc gagtattaat ttcgttaata atctgcatat ggcgcggcag taagcctttc
     7981 accagtttca catcccagcg ttccagcgct tctggcatca gggtatggtt ggtgtaagcg
     8041 aaagttttgc tggtgatggc ccaggcgtca tcccagctca tctggtgctc atcggtcagc
     8101 acgcgcagca gttctggaat cgcgatagtt ggatgggtat cgttcagctg aataacttcg
     8161 taatccgcca gttcgtgcag tttacgcccc gccagatgat ggcgacgcaa aatatccgct
     8221 accgaacagg cacactggaa gtattgctgc atcaggcgca gctttttacc ggcagtatgg
     8281 ttgtcgtttg gatagagaac tttggtcagt ttttccgcat tgatgccttg ctgttcggcg
     8341 cgcaggaaat caccgtcgtt aaatttagtt agatcaaacg gatgcgcatg cgtcgcctgc
     8401 cacagacgca gcggctgcgc cacgccatta cgatagccga caacggggag atcccacgct
     8461 tgaccggtaa tggtaaactc cggctcccag cgtccgtctt tcatcacttt accgccaatc
     8521 cctacctgca catccagtgc ttcgttgtgg cggaaccacg ggtagttact gcgatgccag
     8581 tcatccggcg cttcaacctg tttgccatcg acaaaagact ggcggaacaa accatattga
     8641 tagttcagac cgtaacccgt cgcagactga ccgacagttg ccattgagtc gaggaagcac
     8701 gccgccagac gtcccagacc accgttaccc agcgccgggt cgatctcttc ttccagcagg
     8761 tcagtcaggt tgatgtcata agccttcaac gaatcctgta catcctgata ccagccgaga
     8821 ttcaacaggt tgttgcccgt cagacgacca atcaaaaact ccattgagat gtagttaaca
     8881 tgtcgctgat tcgccaccgg tttggcgaat ggctgagcac gcagcatttc ggccagtgct
     8941 tcactcactg ccagccacca ctggcgagga gtcatttcag ccgcagaatt taagccataa
     9001 cgctgccact gacgtgaaag cacttcctga aattgcttat cgttaaaaat aggttgtgac
     9061 ataggagttc cacttttctt agattttcaa cacaacgtta tcgctagttt gccaggctcg
     9121 atgttgacct tcctcatcct gcgggggatt aggcagggag gagttgcggg gatgagcaag
     9181 gaaatgtgat ctcgaccact taaagctagt gcaaaccaca ggattagcag caaatcaatg
     9241 ccatacagcg cagaaaatct gtatctaagt gcaaaaaatg gccgttgcgt attttcaaaa
     9301 agcggaaggt aactctataa attaagtaaa ggagtgaaac agtttcacaa gtaaaatatc
     9361 cagtgtgctc catctcattc ttaatagatt tattaagatc atctttttag atggcacttt
     9421 catcaggaat gaagaagaaa cccttgctta aatgaatctg atgaacataa gggaaaccag
     9481 tattcacgct ggatcagcgt cgttttaggt gagttgttaa taaagatttg gaattgtgac
     9541 acagtgcaaa ttcagacaca taaaaaaacg tcatcgcttg cattagaaag gtttctggcc
     9601 gaccttataa ccattaatta cgaagcgcaa aaaaaataat atttcctcat tttccacagt
     9661 gaagtgatta actatgctga ttccgtcaaa actaagtcgt ccggttcgac tcgaccatac
     9721 cgtggttcgt gagcgcctgc tggctaaact ttccggcgcg aacaacttcc ggctggcgct
     9781 gatcacgagt cctgcgggtt acggaaagac caccctcatt tcccagtggg cggcaggcaa
     9841 aaacgatatc ggctggtact cgctggatga aggtgataac cagcaagagc gtttcgccag
     9901 ctatctcatt gccgccgtgc agcaggcaac caacggtcac tgtgcgatat gtgagacgat
     9961 ggagcaaaaa cggcaatatg ccagcctgac gtcactcttc gcccagcttt tcattgagct
    10021 ggcggaatgg catagcccac tttatctggt catcgatgac tatcatctga tcactaatcc
    10081 agtgatccac gagtcaatgc gcttctttat tcgccatcaa ccagaaaatc tcaccctggt
    10141 ggtgttgtca cgcaaccttc cgcaactggg cattgccaat ctgcgtgttc gtgaccaact
    10201 gctggaaatt ggcagtcagc aactggcatt tacccatcag gaagcgaagc agttttttga
    10261 ttgccgtctg tcatcgccga ttgaagccgc agaaagcagt cggatttgcg atgacgtttc
    10321 cggttgggcg acggcactat agctaatcgc cctctccgcc cggcagaata cccactcagc
    10381 ccataagtcg gcacgccgcc tggcgggaat caatgccagc catctttcgg attatctggt
    10441 cgatgaggtt ttggataacg tcgatctcgc aacgcgccat tttctgttga aaagcgccat
    10501 tttgcgctca atgaacgatg ccctcatcac ccgtgtgacc ggcgaagaaa acgggcaaat
    10561 gcgtctcgaa gagattgagc gtcaggggct gtttttacag cggatggatg ataccggcga
    10621 gtggttctgc tatcacccgc tgtttggcaa cttcctgcgc cagcgctgcc agtgggaact
    10681 ggcggcggag ctgccggaaa tccaccgtgc cgccgcagaa agctggatgg cccagggatt
    10741 tcccagcgaa gcgattcatc atgcgctggc ggcaggcgat gcgctgatgc tgcgcgatat
    10801 tctgcttaat cacgcctgga gtttgttcaa ccatagcgaa ctgtcgctgc tggaagagtc
    10861 gcttaaggcc ctgccgtggg acagtttgct ggaaaatccg cagttagtgt tattgcaggc
    10921 gtggctaatg caaagccaac atcgctatgg cgaagttaac accctgctag cccgtgctga
    10981 acatgaaatc aaggacatca gagaaggcac catgcatgca gaatttaacg ctctgcgcgc
    11041 ccaggtggcg attaacgatg gtaatcagga tgaagcggaa cggctggcaa aactggcact
    11101 ggaagagctg ccgccgggct ggttctatag ccgcattgtg gcaacctcgg tgctgggtga
    11161 agtgctgcac tgcaaaggcg aattgacccg ctcactggcg ctagtgcagc aaaccgaaca
    11221 gatggcacgc cagcacgacg tctggcacta cgccttatgg agtttaattc agcaaagtga
    11281 aattctgttt gcccaagggt tcctgcaaac cgcgtgggaa acgcaggaaa aagcgttcca
    11341 gctgatcaac gagcagcatc tggaacagct gccaatgcat gagtttctgg tgcgcatccg
    11401 agcacaactg ttatgggcct gggcacggct ggatgaagcc gaagcgtcgg cgcgtagcgg
    11461 gattgaagtc ttgtcgtctt atcagccaca gcaacagctt cagtgcctgg caatgttgat
    11521 tcaatgctcg ctggcccgtg gtgatttaga taacgcccgt agccagttga accgtctgga
    11581 aaacctgctg gggaatggca aatatcacag cgactggatc tctaacgcca acaaagtccg
    11641 ggtgatttac tggcaaatga ccggcgataa agccgccgct gccaactggt tgcgtcatac
    11701 ggctaaaccg gagtttgcga acaaccactt cctgcaaggt caatggcgca acattgcccg
    11761 cgcacaaatc ttgctgggcg agtttgaacc tgcggaaatt gttctcgaag aactcaatga
    11821 aaatgcccgc agtctgcggt tgatgagcga tctcaaccgt aacctgttgc tgcttaatca
    11881 actgtactgg caggccggac gtaaaagtga cgcccagcgc gtgttgctgg acgcattaaa
    11941 actggcgaat cgcaccggat ttatcagtca ttttgtcatc gaaggcgaag cgatggcgca
    12001 acaactgcgc cagctgattc aacttaatac gctgccggaa ctggaacagc atcgcgcgca
    12061 gcgtattctg cgagaaatca atcaacatca tcggcataaa ttcgcccatt tcgatgagaa
    12121 tttcgttgaa cgtctgctaa atcatccgga agtgccagaa ctaatccgca ccagcccgtt
    12181 gacccaacgt gaatggcagg tcctggggct gatctactcc ggttacagca atgaacaaat
    12241 tgcgggcgaa ctggaagtgg cggcaaccac catcaagacg catatccgca atctgtacca
    12301 aaaactcggc gtcgcccacc gtcaggctgc cgtgcagcac gcgcagaaac tgctgaagat
    12361 gatgggttat ggggtgtgag tttagccgga taatgcattg gatccggttt acatctttac
    12421 atcattcaat actcacccgc gttacgccat ctgtttctat caaaccaaac cgcaccggca
    12481 agaaacgctc caccaccgcg atattggtca gcagatggca tgaaggatgg gcgaccgtaa
    12541 attctcccgc gcccgccagc gccatcggca gtaccagttg gtcggcgaga tattccccca
    12601 ccgccgctgg gcttgccagg tagcgtttca cctctttcac taactgcgcg gctaccacct
    12661 cggcactgac gcgcttttca ccgacgacga aaaagcgttc agtgatattt tcactttcga
    12721 cttccagcga gacggtatta cccggcccct ggtcgcgcgg caggttatga atattctgtt
    12781 catgcaggga aaaaatcccc gccagtgtag cgatttcacg ctcagcaaca tggcgcggca
    12841 caccagctaa tagaacttct ccacgcatct gcacaatgtt cccgcgctcg ccaagttgca
    12901 aggtgctaaa cgatgccacg ggcgagactt ccgttgccac cacaccgcct ccggcaggat
    12961 aaaaaccgtg acgtaacagc gtggtttgct gatgaattcc tattttcgcc agcagcggct
    13021 ccagcacccg gcggataaaa tccgcaggcg gcgccgacgg gttatcggtg ccgccgctca
    13081 cttcaacacg cgaaggtcca tcggcaaacc acagcgcggg cagcaccgtt tgcagcacca
    13141 gcgtacaact tccggcgcta ccgatagcaa agcggtaatc gccgccgcgc acggtgccag
    13201 gccggaagac cagacgctgc gatcccagct ccgcgccttc caccgttgcc ctacaaattt
    13261 ccgcagccgc ttttaccgcg gtcagatgct ggcgcagcag ccccggtttc gcccgtccgg
    13321 ctcgaatgcc agtgatggta aatggctggc cggttatcat cgacaggctc agcgccgagc
    13381 gcaggatctg cccgccgcct tcgccctgtg cgccatccag cgcaatcatc cttttcatca
    13441 tttatccttt tacgcacacc acctgacgca gggtgtagat aacttccacc agatcgcttt
    13501 gtgccgccat caccgcatca atatctttat acgccatcgg gatttcgtcg atcacttcgg
    13561 catctttacg gcattccaca tgcgcggtgg cacgaatttg gtcttccacg ctgaacagtt
    13621 ttttcgcttt agttcggctc attacccgcc cggcaccgtg gctgcacgaa cagaacgact
    13681 cttcatttcc cagcccacgg acgataaagc tttttgctcc catcgaaccg ggaataattc
    13741 catattgacc agcacgcgca gacaccgcac ctttacgcgt cacgtagatc tcttcaccaa
    13801 agagctgttc tttttgcaca tagttgtggt gacagttgat ctcttccatc gccagcgttt
    13861 gtggctgtct gaccgttttc tgcgtaatgc tctgcaacgc cgttaccaca ttttccatca
    13921 tcgcatcgcg gttaaggctg gcgaaaagct gcgcccaggc cacggctctt aggtaatcat
    13981 caaagtattc cgtaccttcc ataaagtacg ccagatcacg cgacggcaac gtctcaagtt
    14041 ggtcctgcat ctctttttgc gccagatcga taaagtaagt cccgatggcg ttaccaattc
    14101 cgcgtgaacc ggagtgcagc ataatccaca cctgctccga ctcatcaagg cagatttcaa
    14161 taaagtggtt acccgttccc agcgttccca ggtgtttata gttgttagtg ttaaggaaac
    14221 gcggatattt ttgcgttaac cactgataac cggcctccag ctcagcccat ttcgcatcga
    14281 cgttaaaagg cgggttttcc catgcgcctt tatcacgttt acaacgcccg gtagtacgcc
    14341 cgtgtggtac ggccgtttca atcgcttgac gcagctccgc caggttttca ggcaggtctg
    14401 ccgccgttaa cgcggtacgc agcgcgttca ttccacagcc aatatccacg cccaccgccg
    14461 ccggaataat cgcccctttg gtcgggatca cgctaccaat ggtggaacct ttacccaggt
    14521 gtacatcagg cattaccgca atatgtttga aaataaacgg catcttcgcc gtattaataa
    14581 gttgctgacg cgcatcggct tctaccggca cgcctttggt ccacattttt accggggcat
    14641 tttcagtggt cagtaattcg taattcattt tgtttttctc ttttcgttgt ttgcggtcct
    14701 gataattgca accgtcgtgc cagaaaatta aataagcaga ttaatttatt gtttctaaaa
    14761 agattatatc tttatatccg taaacggaga tttcccgcaa agccgattta ccgataatga
    14821 aatatcgcct tttataagga tatctaagat gcgtaaaaca gtggcttttg gctttgtcgg
    14881 taccgtgctg gattatgccg gacgcggcag tcagcgctgg tcaaaatggc gtccgtcact
    14941 ctgtatatac cagcaagaat cgttggtcat cgatcgactg gaattattgc acgatgcccg
    15001 ctcgcgctcg ctatttgaaa cgcttaaacg cgatatcgcc agcgtttcgc cagaaacaga
    15061 agtggtgggc gttgagattg aactgcataa cccgtgggat ttcgaagagg tttacgcctg
    15121 cctgcatgat ttcgcccgtg gttacgagtt tcagccagaa aaagaagact atttaattca
    15181 catcaccacc ggcacccacg tcgcgcagat ttgctggttt ctgctggcgg aagcgcgtta
    15241 tctacccgcc cggttgatac aatcttcaac tccgcgcaaa aaagaacagc cgcgcggccc
    15301 aggtgaagtg acgattatcg atctcgattt aagccgttat aacgccatcg ccagccgctt
    15361 tgccgaggaa cggcagcaaa cgctcgattt cctcaaatcc ggtattgcca cgcgtaaccc
    15421 ccacttcaac cgcatgattg agcagatcga aaaagtggcg attaaatccc gcgcgccgat
    15481 tctgcttaac ggcccgaccg gcgcaggtaa gtcatttctg gcgcgacgca tcttcgagtt
    15541 aaaacaggca cggcatcagt ttagcggcgc gtttgtggaa gtgaactgcg ccaccctgcg
    15601 cggcgatacc gcgatgtcga cgctgtttgg tcatgtgaaa ggcgcgttta ccggggcgcg
    15661 ggaatctcgt gaaggattat tacgcagcgc caacggcgga atgttgtttc ttgatgagat
    15721 tggcgaactg ggcgcagacg aacaggcaat gctgctgaaa gctattgaag agaaaacctt
    15781 ttacccgttt ggcagcgatc gccaggtgag cagtgatttt cagcttatcg ccggaacggt
    15841 gcgcgatttg cgccagctgg ttgccgaagg caaattccgc gaagatctgt acgcgcggat
    15901 caatctctgg accttcaccc tgccgggtct acgccagcgc caggaagata ttgaaccgaa
    15961 cctggattat gaagtggagc gccacgccac actcactggt gacagcgtgc gttttaacac
    16021 cgaagcgcgg cgtgcctggc tggcctttgc aacatctccc caggcaacat ggcgcggtaa
    16081 ctttcgcgaa ctttctgcca gcgtcacgcg gatggccacc tttgccacta gcggacgcat
    16141 cactctggac gtggtggaag atgagataaa ccgtctgcgc tataactggc aggagagccg
    16201 cccctccgcg cttacggcgt tgctgggcgc agaggcagaa aacatcgatc tcttcgaccg
    16261 tatgcaactg gaacacgtta tcgctatctg ccgccaggca aagtcgcttt ctgctgccgg
    16321 acgacagctt tttgatgttt cgcgccaggg caaagccagc gtcaatgacg cggatcggct
    16381 acgcaaatac ctggcgcgtt ttggtctgac gtgggaagcc gtgcaggatc agcacagctc
    16441 cagttgaata tggtggtccg tcagcacctg catcacgctt actggcggcg gggcgtcggt
    16501 gtagacggca tcaaccatgc tgatgctccc catattgacc atcgcattac ggccaaattt
    16561 tgaatggtca accaccagca taacgtggcg cgagttctca ataatggcgc gcttggtacg
    16621 aacttcgtgg taatcgaact ccagcagcga gccgtcgctg tcgatgccgc tgatccccag
    16681 aatgccgaaa tcaaggcgga actgggagat aaaatcgagc gtcgcttcgc caatgatccc
    16741 gccatcgcgg ctacgtaatt cgccaccggc gagaataatg cggaaatctt cttttaccat
    16801 caacgtgtta gcaacgttga gattgttggt gacaatgcgc aaattgctgt gattgagcag
    16861 cgcgtgcgcc accgcttccg gcgtggtgcc gatatcgata aacagcgtcg agccattggg
    16921 gatttgctcc gccactttgc gggcgatgcg ctctttttct tcggtctggg tggctttacg
    16981 atcgtgccac ggcgtgttaa ccgaactgga aggcagcgcc gcgccgccat gatggcgcag
    17041 gatcaggttt tgctctgcca gctcgttgag gtcgcggcga atagtctgcg ggctgacgga
    17101 gaagtgctcc accagttcct cggtactgac ataaccctgc tgtttaacca gttcgataat
    17161 accgttgtga cgttgtgttt gtttcattta taaatccctg gaattatttt cgttttcgcg
    17221 cattgagcga atcaacaaaa gccatcgcta aacccacggc taatccggcg atgtgtgctc
    17281 cgttcgccat cgacatccca aacaaatcaa accatccggc gacaatccag atcagcgcaa
    17341 agataattaa cccacgttgc aggtaaatgc cactttgcgg atcgcgttcg ccacgtagcc
    17401 agacgtagcc catcagcgca tacaccacgc cagaaagccc gccaaaccac ggcccgctga
    17461 atttttgctg catatagccg cttaacaggg cgctgataag cgtaatgaca attagcttac
    17521 cgctaccgag gcgtttttcc accgcgccgc cgagatacca ccaccaaagc aggttaaaga
    17581 ggatatgcat cagcgagaag tgcattaacg cgtgggtgaa gtaacgccag aactcaaatt
    17641 tcagtgttgg atcgaatggc caggccagcc ataacatcac ttcctgatcg ccgagaattt
    17701 gcatggcaat aaacaccacc acgcaggcga tcatcatcac ccaggtcacc ggacctgcgc
    17761 gttcacgcaa ggcagcaaag aaaggataac ggcgataatg caggccactg ccggtatggc
    17821 ctgccagcca gctggccgcc agataacgcg gatctgccgg gttttcgaga aaacgcgcca
    17881 gctccgcccg cacgcgctcg gcctgggact catccgccag ccagacatcg ctttggttat
    17941 gttgttgaat cgtgaggata acaccctgcg tcgccatgta atcaacaaac gcctgcgcca
    18001 cgcgggggtt agcaaaagag gtaatcatca acatcgttgc tgtcgcttat tccacacaaa
    18061 aggggacagt ataaagcgtt acgcgccgta cgccacctct gcgggaaact gtcgctgcca
    18121 ggcttcaaag ccgccgtcaa tgctatagac cacatcgtag ccctgttgca gcagatactg
    18181 cgccgcccct ttgctgctat tgccgtgata acacatcacc atcaccggag tgtcaaagtc
    18241 gttatcacgc ataaacgcgc ccagcgtgtc gttggttaaa tggaaagcct gcaccgcatg
    18301 ccccatagcg aaactctgtg gatcgcgaat atcgaccagc accgcctctt tttcctgcaa
    18361 cttctggtgc gcgtcggcaa cgttaataca ttcgaactga tccatgcgtc tctctttctt
    18421 tacaaacaag tgggcaaatt taccgcacag tttacgtcga agcggcagat aaacgccata
    18481 atgttataca tatcactcta aaatgttttt tcaatgttac ccaaagcgcg attctttgct
    18541 aatatgttcg ataacgaaca tttatgagct ttaacgaaag tgaatgaggg cagcatggaa
    18601 accaaagatc tgattgtgat aggtggcggc atcaatggtg ctggtatcgc ggcagacgcc
    18661 gctggacgcg gtttatccgt gctgatgctg gaggcgcagg atctcgcttg cgcgacctct
    18721 tccgccagtt caaaactcat tcacggtggc ctgcgctacc ttgagcacta tgaattccgc
    18781 ctggtcagcg aggcgctggc tgaacgtgaa gtgctgctga aaatggctcc gcatatcgcc
    18841 ttcccgatgc gttttcgcct gccacatcgt ccgcatctgc gcccggcgtg gatgattcgc
    18901 attggtctgt ttatgtacga tcatctgggt aaacgcacca gcctgccggg atcaactggt
    18961 ttgcgttttg gcgcaaattc agtgttaaaa ccggaaatta agcgcggatt cgaatattct
    19021 gactgttggg tagacgacgc ccgtctggta ctcgccaacg ccctgatggt ggtgcgtaaa
    19081 ggcggcgaag tgcttactcg gactcgcgcc acctctgctc gccgcgaaaa cggcctgtgg
    19141 attgtggaag cggaagatat cgataccggc aaaaaatata gctggcaagc gcgcggcctg
    19201 gttaacgcca ccggcccgtg ggtgaaacag ttcttcgacg aggggatgca tctgccttcg
    19261 ccttatggca ttcgcctgat caaaggcagc catattgtgg tgccgcgcgt gcatacccaa
    19321 aagcaggcct acattctgca aaacgaagat aaacgcattg tgttcgtgat cccgtggatg
    19381 gacgagtttt ccatcatcgg caccaccgat gtggaataca aaggtgatcc gaaagcagtg
    19441 aagattgaag agagtgaaat caattacctg ctgaaagtgt ataacgcgca ctttaaaaag
    19501 cagctaagcc gtgacgatat cgtctggacc tactccggtg tgcgtccgct gtgtgatgat
    19561 gagtccgact cgccgcaagc cattacccgt gattacaccc ttgatatcca tgatgaaaac
    19621 ggcaaagcac cgctgctgtc ggtattcggc ggtaagctga ccacctaccg aaaactggca
    19681 gaacatgcgc tggaaaaact gacgccgtat tatcagggta ttggcccggc gtggacgaaa
    19741 gagagcgtgc tgccgggtgg tgccattgaa ggcgaccgcg acgattacgc cgctcgcctg
    19801 cgccgccgtt acccgttcct gactgaatcg ctggcacgtc actacacgcg cacttacggc
    19861 agcaacagcg agctgctgct cggcaatgcg ggaacggtaa gcgatctcgg ggaagatttc
    19921 ggtcatgagt tctacgaagc ggaactgaaa tacctggtgg atcacgaatg ggtccgccgc
    19981 accgacgatg ccctgtggcg tcgcacaaaa caaggcatgt ggctgaatgc ggatcaacaa
    20041 tctcgtgtga gtcagtggct ggtggaatat acgcagcaga agttgtcgct ggcgtcgtaa
    20101 attaacgtaa ggtgatcagg tcagatttca atctggcctg agactgatga caaacacaaa
    20161 actgcctgat gcgctacgct tatcaggcct acgtggttta tgcaatatat tgaatttgca
    20221 tggtcttgta ggccagataa gacgttcacg tcgcatccgg catgaacaaa gcgcactttg
    20281 tcaaaaatct aacctacttt ttaattcagg aaattaccgc aaagcccacg ccatcatacc
    20341 caacgtaaca aaactcaggc acgttcccca ctcgccccga gaaaaatagc attaatgcgc
    20401 ccagcgtcag cataaaaata ttgagcagaa ggtaaattgt tggcgtaaag agtaaattgc
    20461 aaacctcagg aatagcggca agcccagccc ccagcatggc aataaccgct gcaaaaaatt
    20521 gcgtcagtat tgggatgccg aaaaaagtca ttaccagcga ggtcaaaatc cattttctat
    20581 tttgcattat tctttccatt ctttctgaat ggcgaaatta tactccccga gtccccttgc
    20641 cccttctgga cacttttccg aaatgatggc gaaaaaaacg ggaccctttg g
//
LOCUS       DAZPCS010000057        20577 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZPCS010000057 DAZPCS010000000
VERSION     DAZPCS010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20577
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.057"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            112..1167
                     /gene="phoE"
                     /locus_tag="ACHUQ7_002063"
     CDS             112..1167
                     /gene="phoE"
                     /locus_tag="ACHUQ7_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFR1135477.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
     gene            complement(1206..1607)
                     /gene="crl"
                     /locus_tag="ACHUQ7_002064"
     CDS             complement(1206..1607)
                     /gene="crl"
                     /locus_tag="ACHUQ7_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFR1135478.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(1665..2909)
                     /gene="frsA"
                     /locus_tag="ACHUQ7_002065"
     CDS             complement(1665..2909)
                     /gene="frsA"
                     /locus_tag="ACHUQ7_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFR1135479.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            complement(3001..3459)
                     /gene="gpt"
                     /locus_tag="ACHUQ7_002066"
     CDS             complement(3001..3459)
                     /gene="gpt"
                     /locus_tag="ACHUQ7_002066"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFR1135480.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3721..5178
                     /gene="pepD"
                     /locus_tag="ACHUQ7_002067"
     CDS             3721..5178
                     /gene="pepD"
                     /locus_tag="ACHUQ7_002067"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFR1135481.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(5235..5849)
                     /gene="prfH"
                     /locus_tag="ACHUQ7_002068"
                     /pseudo
     CDS             complement(5235..5849)
                     /gene="prfH"
                     /locus_tag="ACHUQ7_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(5911..6954)
                     /locus_tag="ACHUQ7_002069"
     CDS             complement(5911..6954)
                     /locus_tag="ACHUQ7_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFR1135482.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCQGRPD"
     gene            complement(7200..7652)
                     /locus_tag="ACHUQ7_002070"
     CDS             complement(7200..7652)
                     /locus_tag="ACHUQ7_002070"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR1135483.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTVSQHYSLQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            complement(7662..8060)
                     /gene="yafO"
                     /locus_tag="ACHUQ7_002071"
     CDS             complement(7662..8060)
                     /gene="yafO"
                     /locus_tag="ACHUQ7_002071"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HFR1135484.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            complement(8063..8356)
                     /gene="yafN"
                     /locus_tag="ACHUQ7_002072"
     CDS             complement(8063..8356)
                     /gene="yafN"
                     /locus_tag="ACHUQ7_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HFR1135485.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            complement(8408..9463)
                     /gene="dinB"
                     /locus_tag="ACHUQ7_002073"
     CDS             complement(8408..9463)
                     /gene="dinB"
                     /locus_tag="ACHUQ7_002073"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFR1135486.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRECRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(9534..10319)
                     /gene="lafU"
                     /locus_tag="ACHUQ7_002074"
     CDS             complement(9534..10319)
                     /gene="lafU"
                     /locus_tag="ACHUQ7_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFR1135487.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            10291..12003
                     /locus_tag="ACHUQ7_002075"
                     /pseudo
     CDS             10291..12003
                     /locus_tag="ACHUQ7_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(12220..12717)
                     /gene="rayT"
                     /locus_tag="ACHUQ7_002076"
     CDS             complement(12220..12717)
                     /gene="rayT"
                     /locus_tag="ACHUQ7_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HFR1135488.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTTQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(12893..13414)
                     /locus_tag="ACHUQ7_002077"
                     /pseudo
     CDS             complement(12893..13414)
                     /locus_tag="ACHUQ7_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            13798..14538
                     /gene="dpaA"
                     /locus_tag="ACHUQ7_002078"
     CDS             13798..14538
                     /gene="dpaA"
                     /locus_tag="ACHUQ7_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFR1135489.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVAN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(14509..15276)
                     /locus_tag="ACHUQ7_002079"
     CDS             complement(14509..15276)
                     /locus_tag="ACHUQ7_002079"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFR1135490.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(15482..16060)
                     /gene="lpcA"
                     /locus_tag="ACHUQ7_002080"
     CDS             complement(15482..16060)
                     /gene="lpcA"
                     /locus_tag="ACHUQ7_002080"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFR1135491.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            16300..18744
                     /gene="fadE"
                     /locus_tag="ACHUQ7_002081"
     CDS             16300..18744
                     /gene="fadE"
                     /locus_tag="ACHUQ7_002081"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFR1135492.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLTIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYSHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMALLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDNLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKMAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALTKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(18787..19233)
                     /gene="ivy"
                     /locus_tag="ACHUQ7_002082"
     CDS             complement(18787..19233)
                     /gene="ivy"
                     /locus_tag="ACHUQ7_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFR1135493.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     AIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            19414..20184
                     /gene="yafV"
                     /locus_tag="ACHUQ7_002083"
     CDS             19414..20184
                     /gene="yafV"
                     /locus_tag="ACHUQ7_002083"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFR1135494.1"
                     /translation="MPSLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            20294..20526
                     /locus_tag="ACHUQ7_002084"
                     /pseudo
     CDS             20294..20526
                     /locus_tag="ACHUQ7_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
BASE COUNT         5041 a         5280 c         5289 g         4967 t
ORIGIN      
        1 aatatcatta atctgtaata tatctttaac aatctcaggt taaaaacttt cctgttttca
       61 acggggctct cccgctgaat attcgcgcgt taattaaaat caggaatgaa aatgaaaaag
      121 agcactctgg cattagtggt gatgggcatt gtggcatctg catctgtaca ggctgcagaa
      181 atatataata aagacggtaa taaactggat gtctatggca aagttaaagc catgcattat
      241 atgagtgata acgccagtaa agatggcgac cagagttata tccgttttgg ttttaaaggt
      301 gaaactcaaa ttaacgatca actgactggt tatggtcggt gggaagcgga gtttgccgga
      361 aataaagcgg agagtgatac tgcacagcaa aaaacgcgtc tcgcttttgc cgggttgaaa
      421 tataaagatt tgggttcttt cgattatggt cgtaacctgg gcgcgttgta tgacgtggaa
      481 gcctggaccg atatgttccc ggaatttggt ggcgattcct cggcgcagac cgacaacttt
      541 atgaccaaac gcgccagcgg tctggcgacg tatcggaaca ccgacttctt cggcgttatc
      601 gatggcctga acttaaccct gcaatatcaa gggaaaaacg aaaaccgcga cgttaaaaag
      661 caaaacggcg atggcttcgg cacgtcattg acatatgact ttggcggcag cgatttcgcc
      721 attagtgggg cctataccaa ctcagatcgc accaacgagc agaacctgca aagccgtggc
      781 acaggcaagc gtgcagaagc atgggcaaca ggtctgaaat acgatgccaa taatatttat
      841 ctggcaactt tctattctga aacacgcaaa atgacgccaa taactggcgg ctttgccaat
      901 aagacacaga actttgaagc ggtcgctcaa taccagtttg actttggtct gcgtccatcg
      961 ctgggttatg tcttatcgaa agggaaagat attgagggca tcggtgatga agatctggtc
     1021 aattatatcg acgtcggtgc tacgtattat ttcaacaaaa atatgtcagc gtttgttgat
     1081 tataaaatca accaactgga tagcgataat aagctgaata ttaataatga tgatactgtc
     1141 gcggttggga tgacgtatca gttttaatga atattgccgg atgtgatgca tccggcacat
     1201 ttcactcacg ccgttaactt caccggttca tcacgaaaat cattcgccgg ttccagcttc
     1261 agattcagcg tcgtcagcag ttcacgcagc ttctcgtgaa actcacgcag ggtgtgctcc
     1321 agtcgttcaa ccacttcagt atcttttacc ggaacactct tccagtcgcc tgctttatcg
     1381 aacagaccaa actggtagct gtaggtaaaa cgggattcct gcgcttccag ctccatccac
     1441 cagccccaga attcacgcac ttccggtgcc ggtttcacgt tgacgcatac agccagacaa
     1501 tcgaaaaaga atcgattatc ttcgcactta ccttcacgaa tatacgggcc tagtgcggta
     1561 aattttttga tcaatctgct cttcgggtgt ccactcggta acgtcattgc tatctcctgt
     1621 tgtgatgcaa ctgttttacc aaattggcaa aatttagcaa atttttaaca caagcgtttt
     1681 tcgatccagc cggtgatttc ctgaagacct ttgtcaaaat tccgatacac cgggttaaat
     1741 gggatctcta ataatttacc gtcagcagat gatgaggtga ttaagcgtga gtcctcttcc
     1801 gggctgaacg gatcgttctt ccagtagcct gataacattg gcgttgggca gcgacgcccc
     1861 agcaatcctt gcacttttaa tgaatagcga ttcagctcca cgcgtaacgc ttcgtcggaa
     1921 gcatcatgca tccccaaacg actcgccaga acgtcaagat acatttccgg cacctgttgc
     1981 tggcacttaa aatcactcaa cagggtatga actaccggac caagacaggc aaccgctttc
     2041 agacgcggcg attcaaggta tgccagacgc acggcaacgt tagcgccgaa acggaaacca
     2101 aaggccgcga cgcgagtgtg atccacccac ggtacgttag gcagcgcctt taagacgtgc
     2161 tgatgcaaca ggctggagtc ctgggtgagc ttccattttg aagaaaagcc caccgacggc
     2221 atatcaatag tcagcatcgc aatgccgcgc ggcgcaaaat aacgttcata caggctgtaa
     2281 tagtccgtct gcatcgcatc cagaccacca cacattaata ctgtcgggaa cgggccatcg
     2341 cctttcggca tatgcaaaaa gccggtgatg ggcgcaccgc cgggtacggt aaactccatc
     2401 tgccgcatcg tgcccggcag acgctgggcg gcctcttcat aggcgcggtt tgataaagcc
     2461 tgcgcttgct cggccaggtc atctcctttc agatgaggat aggcggcaat gttgtacaac
     2521 gtagccgcat gcagccagtg tcgaccactg agttgcggat cttcttccgt acaggctttt
     2581 tgttgccaca ccatcgcctg ggtggcccac tcataaatcc agttgccacc acggtagcca
     2641 atcacggtat cgtataaatc atcgtcggta cgttcagcat cgctcatcac aatacgtgcc
     2701 tggacgtcga ggatttcacg tgggtcaata ccgcgccaga tccacatcag acggttaatc
     2761 atgcgatacc agtgagggat ggttttacca tcaagagccg actgcacagg cggttgtgcg
     2821 ccgtgattaa agcggcggac tagcgtcgag gtttcaggat gtttaaagcg gggtttgaac
     2881 agggtttcgc tcaggtttgc ctgtgtcatg gatgcagcct ccagaatact tactggaaac
     2941 tattgtaacc tgcctgaagt taaaaagaac aacgcccggc aatgccaggc gttgaaaaga
     3001 ttagcgaccg gagattggcg ggacgaatac gacgcccata tcccacggct gttcgatcca
     3061 ggtatcttgc gggatatcaa caacatagtc atcaaccagc ggacgaccag ccggttttgc
     3121 gaagatggtg acaaagtgcg cttttggata catttcacga atcgcaacag cagtaccacc
     3181 ggtatccacc aggtcatcaa taacgatgaa gccttcgcca tcgccttctg cgcgtttcag
     3241 cactttaagc tcgcgctggt tgtcgtgatc gtagctggaa atacaaacgg tatcgacatg
     3301 acgaataccc agttcacgcg ccagtaacgc ccccggtacc agaccgccac ggcttacggc
     3361 aataatgcct ttccattgtt cagaaggcat cagtcggctt gcgagtttac gtgcatggat
     3421 ctgcaacatg tcccaggtga cgatgtattt ttcgctcatg tgaagtgtcc cagcctgttt
     3481 atctacggct taaaaagtgt tcgaggggaa aataggttgc gcgagattat agagatctgg
     3541 cgcactaaaa accagtattt cacatgagtc cgcgtctttt tacgcactgc ctctccctga
     3601 cgcgggataa agtggtattc tcaatacata tctcgcaagc ctgtcttgtg ttgacaacat
     3661 tttctgctaa ccctgtgacc tgcaatactg ttttgcgggt gatcgacaag gagacttaac
     3721 gtgtctgaac tgtctcaatt atctccacag ccgctgtggg atatttttgc caaaatctgt
     3781 tctattcctc acccgtccta tcatgaagag caactcgctg aatacattgt tggttgggca
     3841 aaagagaaag gtttccatgt cgaacgcgat caggtaggta atatcctgat tcgtcaacct
     3901 gctaccgcag gtatggaaaa tcgtaaaccg gtcgtcttac aggcccacct cgatatggtg
     3961 ccgcagaaaa ataacgacac cgtgcatgac ttcacgaaag atcctatcca gccttatatt
     4021 gatggcgaat gggttaaagc gcgcggcacc acgctgggtg cggataacgg cattggtatg
     4081 gcctctgcac tggcggttct ggctgacgaa aacgtggttc acggcccgct ggaagtgctg
     4141 ctgaccatga ccgaagaagc cggtatggac ggtgcgttcg gcttacagag caactggttg
     4201 caggctgata ttctgattaa caccgactcc gaagaagaag gtgaaatcta catgggttgt
     4261 gcggggggta tcgacttcac ctccaacctg catttagatc gtgaagcggt tccagctggt
     4321 tttgaaacct tcaagttaac cttaaaaggt ctgaaaggcg gtcactccgg cggtgaaatc
     4381 cacgttggcc tgggtaatgc caacaaactg ctggtgcgct tcctggcggg tcatgcagaa
     4441 gaactggacc tgcgccttat cgatttcaac ggcggcacac tgcgtaacgc catcccgcgt
     4501 gaagcctttg cgaccattgc tgtcgcagct gataaagtcg acgcactgaa atctctggtg
     4561 aatacctatc aggagatcct gaaaaacgag ctggcagaga aagagaaaaa tctggccttg
     4621 ttgctggact ctgtagcgaa cgataaagct gccctgattg cgaaatctcg cgataccttt
     4681 attcgtctgc tgaacgccac cccgaacggt gtgatccgca attccgacgt ggcaaaaggt
     4741 gtggtcgaaa cctccctgaa cgtcggtgtg gtgaccatga ctgacaataa cgtagaaatt
     4801 cactgcctga tccgttcact aatcgacagc ggtaaagact acgtggtgag catgctggat
     4861 tcgctgggta aactggctgg cgcgaaaacc gaagcgaaag gcgcatatcc tggctggcag
     4921 ccggacgcta attctccggt gatgcatctg gtacgtgaaa cctatcagcg cctgttcaac
     4981 aagacgccga acatccagat tatccacgcg ggcctggaat gtggtctgtt caaaaaaccg
     5041 tatccggaaa tggacatggt ttctatcggg ccaactatca ccggtccaca ctctccggat
     5101 gagcaagttc acatcgaaag cgtaggtcat tactggacac tgctgactga actgctgaaa
     5161 gaaattccgg cgaagtaatt atttgatttg ctgccggatg gcgtttaatc gccttccggc
     5221 agtttcatcc ttcattatcc ttcgataaaa gccatccctg taaacgttcg tcgcgggttg
     5281 ccacgttcaa tctggtgatg gaacattcgc cgctgtgatt tcagcaccgc gctattttcc
     5341 tattgctgct gatccagctt ccaggcaatc agcaatcgcg ccagccgttt gttagcatgc
     5401 tggctgcgct ctgactgaac cttcacgcta ataccggatg ccaaatgcgt ggcgcgtacc
     5461 gccgagtcgg ttttattgac atgttgaccg cccggccccg acgaacgcag cgtctcatac
     5521 cggatcgcat cggattgctc ctgctcatca gcggtaaaac gcccaatgcc cagaaaccag
     5581 tttttacgcc cgtgatgtgg gcgatacggg ctcgggcaaa tccactgaat agtgccacac
     5641 cagctttcgc ttaacgccca tgcgttgtcg ccatccagag aaatcagcgc cgaacgcagc
     5701 gtgtcagagt agcggcctgt ttctgtttcc agcaccgtta ccgcgacgtc ttgtcgggca
     5761 gcttctttaa ttagcctgtc cagcgccttt ttcactgcga gacaacattc ttccggcccc
     5821 tgcgctgagg agagttatag caagatcatc cttttttccc tccactgttt ttgagcgtta
     5881 gcaccggacg cagccgcgcg acaggaataa ttaatccggc ctgccctgac attgcaccac
     5941 actttcagcc gatttatagg cttgtggcgc ttcttcgaag atgagttgtt tatcgcgaca
     6001 aattacccga ctgccaagtt cagtacgcga gagttgcgtc gctgtgtatt tcgctgccag
     6061 acgcccttta cactcggtgc gcccccattt acgcccagcc ccatgcgcca gcgaatgcaa
     6121 tgtttcctca ctcctgacgg gctgaaccag cctggagtaa tcaccgcgtg aaccgggaat
     6181 aaccaccaga ccacagtcat caggcgtggc acctttacga tgtaagaccc cttgttgatc
     6241 accgattcga cacgcgctaa caaagttatg cgccacatcc agaaccggac ttccggtggc
     6301 cttaacctgt tgcattatgc gcaaagcgat caggtggcga ttaaaacgcg caaacgccag
     6361 cgcgtcgtca ctgccttcag gcaaaccatg atgcgaaaac gaggcaatat gccgctgtaa
     6421 aatagactgc cctagccccc tcgagccgct atgaaccagc agttgcagat gctgcgcatc
     6481 cagacctgcc agtgcaaaca gttcagcgtt gatgatttga tcaacttgtt gcagttcagc
     6541 gaaatgattg ccaccaccga tagagccaag cgagctgcgc cacggatgct gtgcaaatgc
     6601 tgacggcagg ttttcctcca gccagctttc ttcagcaacg tcatccaaag ctgataatcg
     6661 cttttcaaac ttatcggcgt tgtatttgcg agcgagaata tctgtttgcc atagcgccat
     6721 gccacagccg atatcattgc cgaccagtgc tgggtaaaaa cggcctgcgg agaagaacgc
     6781 tgcgccaatc gggtagccgc gtccggggtg taaatctggc atccccacta cgcgctgcat
     6841 gttgggtaaa tttgccgtgg tgtgtaattg ttggatcgct aaactctcga tccacaggtc
     6901 atcagatgcg atggtaaata ccgcatcgga taagggacga atataattgc ccatatacca
     6961 ttccgtaaat agactcgtca tacttcaagt tgcatgtgct gcgacttgaa ttattgagag
     7021 taacaattaa aaaatcagga aatggcagac gaatccaggg caagaaaaaa agaattaatc
     7081 ctggaaaggt ctgcaaagag tcattgataa aggatttgtc atgatttacc tccttgcagt
     7141 ttgtggaata agaaaacgcg cacatactag gcgggcgtta ttttcattgc aagctggatt
     7201 taatgttgcg gtttataacg catataaaaa ttaataaacc acgctccccg gcattcaacg
     7261 cgctgctgct taactgtctg aaaaccataa cgttcaaaaa agggttttgc ggttatgctt
     7321 gcgtccaccg taagttcgga ttcagactta ataaaaggtt ttaacaaagc gctggcaacc
     7381 ccacggcggg tgtattcagg gtcaacaaat aacatatcga tatagtgttc aacgcaggta
     7441 ataaagccga ccagttttgc attaataact gcaacccgca cttgtgattt cgcgagtttc
     7501 tccttccagc gagattcgtc aatctgcgcc caggcggcaa tttgttgtag tgaataatgc
     7561 tgactgacgg tcatcgtaac cgctctaatg aaaatagcgc atagttgctg aaaatcgcca
     7621 ggctgataat ctcttatttg tatgttattc ataatataaa ttcaaaaacg catgcgaaac
     7681 gcttctgcca tttttccaat tttatgcatt tggttgttat ctcgagccag tttatgaggt
     7741 tcaggcttca gaatggcaat gagcaaccat gcttgctcat caaacgcacc ctgacaatat
     7801 accaaatgcg cttcgtcatt cgttctgctg aattggcgca actgtggcgg aaatggatta
     7861 ttcacatttg ccagatgaat atgagcaact cgctcaaatt tgattaatgg ccaggtaaac
     7921 gagtcgtcgt agagtgcatc gcgaccaaat atatctggca aaacaccgtc acgcttatag
     7981 gaaataaaat ccgccgttaa cgcatcaagt tcctctgctg taagttgcag gcgaataagt
     8041 tttgttttga atacccgcat ccttattcct taaagtcatt gaaatcatca tccgtcatat
     8101 cattaagata ttgtttagcg cggcgtgtaa tttcctctaa tcttgcagca cttggacgaa
     8161 agcgcgcctt tatgggcttt ttctcctctt gttcagcaag catgtccatt aacgcttcga
     8221 atgcgctggc acttaagaga tatcctgcgg ggcgattatt agaaagaacc gcaactggtt
     8281 gatcaataaa gtatttagct gggtttttac gtaactcagt gatattgacc gatttttcag
     8341 cgagaattcg atgcatacag tgataccctc ataataatgc acaccagaat atacataata
     8401 gtatacatca taatcccagc accagttgtc tttccatttg cgggtcaagc aacgtcacat
     8461 gcagccccac cagacgcaca ccgcgcccgc cgcggcgttc atcccaggtt ttacgcgcgg
     8521 tggcgattaa gtcagcttta ttcagccgcg gccagacgtg ctcctgggtg gtctgctgaa
     8581 aatcgtcgaa ctttaatttc accccctggc gagcaatcag taaatcaggt tttacctttg
     8641 ccagacggcg ttcaagttcc ggatacagcc gctcgataat cgcttcacat tcagaccagt
     8701 gatgaatatc ttccgccatc gtgcgttcca cgccgacgga ttttcgtaac cgttcgctgt
     8761 taacgtcgcg ctcgtcaatc ccctgactac gctcccacaa aatgcggcca aatttgccaa
     8821 agcgtttaag cagaatcacc agatcacact tttgtacatc accgcaggtc cgcagcccca
     8881 tcgcttccag ttttgccgcc gagactttgc cgacgccggg gattttttcc agtggtaagg
     8941 tttgtaaaaa tgccggaact tctgccggcg taatcacaaa ctgaccgttg ggtttattca
     9001 tgtcggaggc gattttggcg agaaacttta ctggtgccac gcccgcagat gccgtcagtt
     9061 gcagctcgtt gaagatcgtc tggcggattt cctgggcgat gagggtcgca gaaccgtggc
     9121 aatggacgct atcggtgaca tcgagataag cctcatccag tgacaacggt tcaatgcgcg
     9181 aggtgtagcg cgagaagatt tcacggatat gatttgaggc ttctttgtag gcgtcaaagc
     9241 gccccggaag caaggtgaga tgtgggcata atttgagcgc catcccagta ggcatagcgc
     9301 tacgtacgcc aaatttacgc gcgggataat tggcggtgct gatcaccccc cgacattcgc
     9361 ggctgccgcc aatagcaata gggatatcgc gcagagcggg attgtcgcgc atctccaccg
     9421 ctgcgaaaaa gcagtccata tccacatgaa tgattttacg cattgctcac ctctcaacac
     9481 tggtaaagta tacagtgatt tcaggttttg agaaatgcgt aaagattcag catttagcgc
     9541 attcgctgcg aaagcacctg ctgcttatcc agcttttgca ccagcggctg caccacttta
     9601 tccccatgct gaccaaagta ttgatacagc gtatcggacg cacttttggt cagcaccata
     9661 atctcaatac gccgattgcc cgcgctttgc ggattcttcg cgtccagcag catctggtcc
     9721 gccattgcgc ttacctgcat cactttatct tccggcattc cggcctcttc cagcacccga
     9781 cgagctgaaa gcgcgcgatc gcccgaaagg ttccagttgt tgtagatatt gtttttgtag
     9841 gccatcgcat cggtatgccc ggtaataata attttgttat agagcgagtc gaacactggc
     9901 gcgagctcca ccagcaaagt tttaaagaac ggcataatct gcgcgctgcc gcgttcgaac
     9961 atattgcgat tctggtcgtc tttaatcagc acgcgtaatc cctggggaac aatctccatc
    10021 tccagattcg cttccatatg cgcatcacgg gcaatggtgt tgatactggt tgccaactcg
    10081 ccaagctcgg tggcggattt cttcttcagc agcgccgttt tctcattcac atcacgcgct
    10141 tttttctccg tctcttccgg caccgccacg gtggcaggtt ttggcggatg agacgggctg
    10201 attttattta acggcgacag cccaccaccg ttaaaaatcg actgcccgtg cagcgcggcg
    10261 ataatgcttt cacgctcgga tttactgacg ctgttgacaa tcaactttat cgtcgtcacc
    10321 aaagaggccg agcgtatttc cgaggtttct gcccgcttta ccctggacgc gatgcccggc
    10381 aaacagatgg cgattgacgc cgatcttaac gccggattga tcaaccaggc gcaggcgcaa
    10441 acccggcgta aagatgttgc cagcgaggcc gatttctacg gcgcgatgga cggggcatcg
    10501 aagtttgtac gcggagacgc catcgccggg atgatgattc tggcgatcaa cctgagcggc
    10561 ggcgtctgta tcggcatctt caaatacaac ctgagcgccg acgccgcctt ccagcaatac
    10621 gtgctgatga ccatcggcga cggcctggtg gcgcagatcc cttccctgct gctctccacc
    10681 gcggcggcga ttatcgtcac ccgcgtcagc gacaacggcg atatcgccca tgacgtgcgc
    10741 caccagttgc tggcaagccc gtcggtgctc tacaccgcca ccgggatcat gttcgtgctg
    10801 gcggtggtgc cggggatgcc gcatctgccg tttttgctgt tcagcgccct gcttggcttt
    10861 accggctggc ggatgagcaa acgcccgcag gcggcggagg cggaagagaa aagcctcgaa
    10921 acgctgaccc gcaccatcac tgaaaccagc gaacaacagg tcagttggga aactattccg
    10981 ctgatcgagc ccatcagctt aagcctcggc tacaagctgg tggcgctggt ggacaaagcg
    11041 cagggcaacc cgctcaccca gcggattcgc ggcgttcggc aggtgatttc cgacggtaac
    11101 ggcgtgctgc tgccggagat ccgcattcgg gaaaacttcc gcctcaagcc cagccagtac
    11161 gctattttca tcaacggcat tgaggctgat gaagcggata ttccggcgga taaactgatg
    11221 gcgctgccct ccagcgaaac ctacggcgag attgacggcg tgctggggaa cgacccggcg
    11281 tacgggatac cggtcacctg gatccagcct gcgcagaaag cgaaggctct gaatatgggg
    11341 tatcaggtga tcgacagcgc cagtgtgatt gccacccatg tgaacaagat tgtgcgcagc
    11401 tatattcctg atttgtttaa ctatgatgac atcacgcagt tgcataaccg tttgtcgtcg
    11461 atggcaccgc gcctggcgga ggatttaagc gcggcgctca attacagcca attgctgaaa
    11521 gtgtaccggg cgctgctgac cgaaggcgtt tccctgcgcg atatcgtcac catcgccacc
    11581 gtgctggtcg ccagtagcac ggtgactaaa gatcatattc tgctggcggc cgatgtgcgc
    11641 ctggcgctgc ggcgcagcat tactcatccg ttcgttcgca agcaggagct gacggtgtat
    11701 acgctgaata atgagctgga aaatctgctg accaacgtag tgaatcaggc gcaacaggcc
    11761 ggcaaagtga tgctcgacag cgtgccggtt gacccgaata tgctcaacca gttccagagc
    11821 acgatgccac aggtgaaaga gcagatgaaa gcggcgggga aagatccggt gctgctggtg
    11881 ccgccgcagc tacgcccttt gctgccgcgt tatgcaaggt tgtttgcgcc ggggctgcat
    11941 gtgctgtcgt ataacgaagt gccggatgag ctggagttga agattatggg ggcgttgatg
    12001 taagaatccg gcatcggtgc acaattgccg gatgcggcgt aaacgcctta tccggcctac
    12061 cgttcaggca caagttttta ggcatgataa gacgcgccag cgtcgcatca ggcatctgcg
    12121 cataactgcc ggatgcggcg tgaacgcctt atccggccta ccgttaaagc acgatttgta
    12181 ggccggataa gcgtaagcgc atccggcacc agcgcacagt tataaaatga tacgctcccc
    12241 ggcacttaaa tccgttatgt ctcccgccca atcgatggga tataaccctc gcgcgacatc
    12301 gcgatggaac gttgagaatg gccaatcact cacctgcttt acccagccat gtttctctgg
    12361 atttatataa atataatcaa catgatggcg ataatctttt gtattgcgaa tggcatgctc
    12421 ccaaaaacgc ggttgccaga tatttttcaa tccgcaagca tgggtgaact gctttttaat
    12481 ctcccgccag cgagaggaaa aatcatcatc gctttcgggt aatgtccata tgcaatgcat
    12541 atgctccggc aaaacgaccc aggcgttaat ttcaaaaggt ctgtctcgct taacgttaat
    12601 aatggcgtta cggagcgtct ggaactgggt ggtcagaaga tggcttcgac gattgcgtaa
    12661 atttaccgtg aaaaaccatg ttcccccctt gatgtaataa cgacgatatt ccgacatagc
    12721 gactcctttt tctcaacggt aagaggaaaa ggaaaggccg acaatgacgc tgtgttatat
    12781 cgggaaagcg gctcgcaaaa acgcacagat ttgtaggcat gataagacgc gtcagcgtcg
    12841 catcaggcat ccgtgcacaa ccgccggatg cggcgtaaac gccttatccg gcctacaaaa
    12901 tcatctcttc cgtcaaaatc ctgcgcgcgc ctaaaaaatg gtcctgccag aaaggttcgg
    12961 ctaaccggct tacccgaatg gtttccccgg tgcgcagtga ctcaataaac tgaccgtcgc
    13021 ccagatacac ccccatatgg tcggcaatct cgcggctgtg gatatggaaa aatagcaaat
    13081 ctccccggcg caggtcgtta ttcgccacaa tcgttgcccg acggtagtgg tacatctcat
    13141 tggccgtgcg cgggagtttc gcctcaagga tcttgttgta ggcataaaaa actaacccac
    13201 tacaatcgaa gccttgatcg ggccgcgtgc cgccccagac gtaaggcttg ccaagctgct
    13261 gctccaggcg gtgaatcgcc acttcggtga tgttatgcag gtgatcgctg cttaaaaagt
    13321 ggttgttttc cgccagcgcc tgccagcgtc tgtcactcgc cttgagcggc gcaggaaacc
    13381 attctggatg ctgttttatc tgctcccggt tgtggtggcg ctggcgagta aagggagcaa
    13441 aacgcataac gaaaacacgc ctggatgcaa aaatctgccg agcaagaacg aagacatgaa
    13501 agacataaac cggctgaatg aaggtgagaa ttttaaaaga ccggaggagt aaacgggatt
    13561 attaattgct tatttgcata ttactattga gatttcataa tattaggaaa taaatgatac
    13621 atgcgctttt cggactattg aaaactgccc ctgtgctaaa tttgtaaacc cagcgccgac
    13681 aggcttgcgt aaaactcttt tggtgataat gtgagccttc caaatccaga ctattccgat
    13741 ttgggatcca ttttgccgtc ctggcgtgtg taaccgtttt atcaaggaat aagcagtatg
    13801 cgtaaaatcg cattaattct tgcgatgttg ttgatcccgt gcgtttcgtt tgccggtttg
    13861 ctgggcagca gtagctcgac cacgccagtg agcaaagagt ataagcagca gttgatggga
    13921 tcccctgtct acatccagat cttcaaggaa gaacgtacgc tcgatcttta cgtcaaaatg
    13981 ggcgagcaat atcaactgct cgacagttat aaaatctgta aatattccgg cggcttaggg
    14041 ccaaaacagc gtcagggcga tttcaaaagc ccggaagggt tttatagcgt ccagcgtaat
    14101 cagttaaaac cagacagccg ttactacaaa gcgattaata ttggattccc caatgcttat
    14161 gaccgtgcgc atggttatga agggaaatac ctgatgatcc acggcgattg tgtatccatc
    14221 ggctgctacg caatgaccaa tcagggtatt gatgagatct tccagttcgt tactggtgcg
    14281 ctggtgtttg gtcagccgag cgtgcaggtg agtatttacc cattccgcat gaccgacgcc
    14341 aatatgaagc gccataaata ttccaacttt aaggacttct gggagcagct gaagccgggc
    14401 tacgactact ttgagcaaac ccgtaagcca ccgaccgttt ctgtggccaa tggccgttac
    14461 gtggtcagca agccgttaag ccacgaagtg gtgcagccac aactggcatc aaactacacg
    14521 ctccccgagg caaaataagc gccattcgcc tggcataatt ttttgccagg tttcattgcc
    14581 cgtcagcggc tgtgtcgcaa tcaccgtgac cacatcattc ggtgtggtct gcgagctgaa
    14641 gtcgatttcc acatcctgat ccagcaacgt tgccacgcca aacggcgcgc ggcgggtgat
    14701 ccagtgtaaa ttagtcgagc aatacgccat tacatagcgc ccgtccgaaa gcagcatgtt
    14761 gaaaacgccc ttcgcccgca gttcatccgc cagtgaggcg atatatttaa acactgccgc
    14821 catgttgccc ggcgtgcgcg ggtaacgctg cgttaattta tgcagcagcc agcaaaaggc
    14881 tttttcgctg tcggtttcac cgaccgggcg gaagttgccg gtttccagtg atttgtagcc
    14941 cgtcagttgt ccgttatggg cataagtcca gttacgcccc cataactcgc gggtaaatgg
    15001 gtgagtattt tccagcgcca cctcgccccg attagcctgg cgaatatgag ccaccaccga
    15061 acaggatttt atcgggtagt cctggacaag tttggcgatg ggggaattaa agctgggttg
    15121 tggatcttta aatgtgcgac agcctttacc ttcgtaaaag gtaatgcccc agccatcttt
    15181 atgtggcccg gttccaccac cacgctgtac aagtccggtg aaactaaagc agatatcggt
    15241 agggacgttg gcgctcatcc cgagcaattc gcacatgggt aacctccaca acagcgggag
    15301 cgcatggacc ccggcgaaga ttgatttaag aattttcagg tcggatgcgg cgtaaacgaa
    15361 ttattcggcc tacggttcgg gtacagattt gtaggcatga taagacgcgt cagcatcgca
    15421 tcaggcatca gcgcacaagt gccggatgcg gcgtaaacgc cttatccggc ctacgccaga
    15481 cttacttaac catctctttt tcaattaact ggatcaggat atggatcact ttaatgtgaa
    15541 tctcctgaat gcggtcggca taaccaaagt gcggaacgcg aatttcgata tccgccgtgc
    15601 cagccatttt accgccgtct ttaccggtca gggtgatcac tttcatcccc ttctcacgcg
    15661 ccgctgcgat cgctttgatc acgtttgcag agttaccgga ggtggagatc cccagcagta
    15721 catcgccttc gcgacctacc gcttcaacgt agcgggagaa aatatcattg aaaccgaaat
    15781 cattaccgac gcaggaaata tgactaacgt cagaaatagc aatcgccggg tagcccggac
    15841 ggttttcacg gtagcgaccg gtcaactctt cggcaaagtg catagcgtcg caatgggaac
    15901 cgccgttgcc gcaggaaagc actttgccac cggctttaaa gctgtctgct aacaggaccg
    15961 ccgcgcgctg aatggcgtga atattggcgt catcttttaa aaagttagcc agcgtttccg
    16021 ccgcttcgtt cagttcgtta cgaataagat cctggtacat gaggatatcc ttcagcataa
    16081 atgtaataga caaaatgcag tgtaccggat accgccaaaa gcgagaagta cgggcaggtg
    16141 ctatgaccag gactttttga cctgaagtgc ggataaaaac agcaacaatg tgagctttgt
    16201 tgtaattata ttgtaaacac attgctaaat gtttttacat ccactacaac catatcatca
    16261 caagtggtca gacctcctac aagtaagggg cttttcgtta tgatgatttt gagtattctc
    16321 gctacggttg tcctgctcgg cgcgttgttc tatcaccgcg tgagcttatt tatcagcagt
    16381 ctgattttgc tcgcctggac agccgccctc ggcgttgctg gtctgtggtc ggcgtgggta
    16441 ctggtgcctc tgaccattat cctcgtgcca tttaactttg cgcctatgcg taagtcgatg
    16501 atttccgcgc cggtatttcg cggtttccgt aaggtgatgc cgccgatgtc gcgcactgag
    16561 aaagaagcga ttgatgcggg caccacctgg tgggagggcg acttgttcca gggcaagccg
    16621 gactggaaaa agctgcataa ctatccgcag ccgcgcctga ccgctgaaga gcaagcgttt
    16681 ctcgacggcc cggtagaaga agcctgccgg atggcgaatg atttccagat tactcatgag
    16741 ctggcggatc tgccaccgga gttgtgggcg taccttaaag agcatcgctt cttcgcgatg
    16801 attatcaaaa aagagtacgg cggactggag ttctcagctt atgcccagtc tcgtgtgctg
    16861 caaaaactct ctggcgtgag cgggatcctg gcgattaccg tcggcgtgcc aaactcatta
    16921 ggcccgggcg aactgttgca acattacggc actgacgagc agaaagatca ctatctgccg
    16981 cgtctggcgc gtggtcagga gatcccctgc tttgcactga ccagcccgga agcgggttcc
    17041 gatgcgggcg cgattccgga caccgggatt gtctgcatgg gcgaatggca gggccagcag
    17101 gtgctgggga tgcgtctgac ctggaacaaa cgctacatta cgctggcacc gattgcgacc
    17161 gtgcttgggc tggcgtttaa actctccgac ccggaaaaat tactcggcgg tgcagaagat
    17221 ttaggcatta cctgtgcgct gatcccaacc accacgccgg gcgtggaaat tggtcgtcgc
    17281 cacttcccgc tgaacgtacc gttccagaac ggaccgacgc gcggtaaaga tgtcttcgtg
    17341 ccgatcgatt acatcatcgg cgggccgaaa atggccgggc aaggctggcg gatgctggtg
    17401 gagtgcctct cggtaggccg cggcatcacc ctgccttcca actcaaccgg cggcgtgaaa
    17461 tcggtagcgc tggcaacggg cgcgtattct cacattcgcc gtcagttcaa aatctctatc
    17521 ggtaagatgg aagggattga agagccgctg gcgcgtattg ccgggaacgc ttatgtaatg
    17581 gatgctgcgg catcgctgat tacctacggc attatgctcg gcgaaaaacc tgccgtgctg
    17641 tcggctatcg ttaagtatca ctgtacccac cgcgggcagc agtcgattat tgatgcgatg
    17701 gatattaccg gcggtaaagg cattatgctc gggcaaagca acttcctggc gcgtgcttac
    17761 cagggcgcac cgattgccat caccgttgaa ggggctaaca ttctgacccg cagcatgatg
    17821 atcttcggac aaggagcgat tcgttgccat ccgtacgtgc tggaagagat ggaagcggcg
    17881 aagaacaatg acgtcaacgc gttcgataaa ctgttgttca aacatatcgg tcacgtcggt
    17941 agcaacaaag ttcgcagctt ctggctgggc ctgacgcgcg gtttaaccag cagcacgcca
    18001 accggcgatg ccactaaacg ctactatcag cacctgaacc gcctgagcgc caacctcgcc
    18061 ctgctttctg atgtctcgat ggcactgctg ggcggcagcc tgaaacgtcg tgagcgtatc
    18121 tcggcacgtc tgggggatat tttaagccag ctttacctcg cctctgccgt tctgaagcgt
    18181 tatgacgacg aaggccgtaa tgaagccgat ctgccgctgg tgcactgggg cgtacaggat
    18241 gcgctgtatc aggcagaaca ggcaatggac aatttactgc aaaacttccc gaaccgcgtg
    18301 gttgccgggc tgctgaatgt ggtgatcttc ccgaccggac gtcattatct ggcaccgtct
    18361 gacaagctgg atcataaaat ggcgaagatt ttacaagtgc cgaacgccac tcgttcacgt
    18421 attggtcgcg gtcagtacct gacgccaagc gagcataacc cggttggttt actggaagag
    18481 gcgttggtgg atgtgattgc cgccgacccg attcatcagc ggatctgtaa agagctgggt
    18541 aaaaatctgc catttacccg tctggatgaa ctggcgcaca acgcgctgac gaaggggctg
    18601 attgataaag atgaagccgc tattctggtg aaagcggaag aaagccgtct gcgcagtatt
    18661 aacgttgatg actttgatcc ggaagagctg gcgacgaagc cggtaaagtt gccggagaaa
    18721 gtgcggaaag ttgaagccgc gtaattgaat aacggagccg aaaggctccg tttctttatc
    18781 cgctaattat ttaaaattaa agccatccgg atggttttcc aggctgccgg tcaacgccgc
    18841 gaacagcacc gttttaccat caatcgaaag cgcatcgttc acattcagcc aggtgagttt
    18901 ctcttgcgac gttttctcat caatagccga gaacagcccc gtcatctgat tagatttctc
    18961 ggaccacatc acagcgatac gttgcgagcc acagtcatgc ggtttgcacg cgctcatcac
    19021 ctgatacgtc tcatctccca acgttacggt ttgtgcggga gtataagtac cgcctttcat
    19081 cacccatgca ggtagcttat gcccttgcac catctgatta aacgcagctt tggtggtttc
    19141 gccctttgca aggctgctaa tggttaaatc atcctgcgcc attgcactgg tggcgatgac
    19201 cagtgcggcg actgtcgtta ttgccttaaa catcattcct cccgagctta tcctgcccat
    19261 gttattaacc tcctaaagcc tagcgataac acgataattt gcaaatcaga tatttccaat
    19321 atcagcattt gttatcaatt tattctgcgc taaggcaact gcctgataag gtgttaaagt
    19381 tataaaaccc ttcgcataaa ggagttgtac accgtgccta gtttgaagat tacgcttttg
    19441 cagcaaccgt tggtgtgggt ggatggtcct gccaacctgc gccattttga tcgtcaactg
    19501 gaaggtatta ccgggcgcga tgtgatcgtt ctaccggaga tgtttaccag cggctttgcc
    19561 atggaagcgg cagcttcgtc gcttgcacaa gatgacgtag tgaactggat gacagctaag
    19621 gcgcagcagt gcaatgcgct gattgcaggc agtgttgcat tacaaacgga gtctggttcg
    19681 gttaaccgct ttttgctggt tgagccgggc ggcacggtac atttttatga taagcgtcat
    19741 ctgttccgca tggcagatga gcatctgcat tataaagcgg gcaatgcgcg agtgattgtg
    19801 gaatggcgcg gctggcgtat tttgccgctg gtgtgctacg acttacgttt tccagtgtgg
    19861 tcgcgcaatc tcaacgatta tgacctcgcc ctgtacgtcg ccaactggcc agctccgcgc
    19921 tctctgcact ggcaggcatt gctgacggcc cgcgcgattg agaatcaggc gtatgtggcg
    19981 ggatgcaatc gcgtcggcag cgatggcaac ggctgccatt atcgcggtga cagccgggtg
    20041 attaatccgc aaggcgagat tatcgctacc gccgacgcgc ataaggcaac gcgtattgat
    20101 gcggagctgt cgatggtggc attgcgggaa tatagagaaa agtttccggc atggcgggat
    20161 gcggatgagt ttaggttgtg gtgagtgggg gttgtgttta gggccggggg ctgggctaga
    20221 ttacgaaagc ccctaactta aacacaatat tacgccagaa gtcaccattt ccaaaacaca
    20281 gcgtcgctgt accgtgggtt atctgcccaa ccgcagcgac acgccggtga tcaaaatcag
    20341 cgccaaatgg ctgagaaaag cgggatttga gatcggcacg ggcgttaccg tgaagatctc
    20401 ggagggatgt ttgatcctgc ttgggataac aacgaagtac aggaactgcg cagggaactt
    20461 tatcaggtta aacagccagt taaggggatg cgagacggga tgtttagtgt gctgaacgaa
    20521 agttaaataa taaaaagccg gaaagatctg gaagaaattt ccggttatta tcgttgc
//
LOCUS       DAZPCS010000058        20523 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZPCS010000058 DAZPCS010000000
VERSION     DAZPCS010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20523
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.058"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            245..586
                     /gene="raiA"
                     /locus_tag="ACHUQ7_002085"
     CDS             245..586
                     /gene="raiA"
                     /locus_tag="ACHUQ7_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFR1135495.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    689..814
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            690..737
                     /gene="pheL"
                     /locus_tag="ACHUQ7_002086"
     CDS             690..737
                     /gene="pheL"
                     /locus_tag="ACHUQ7_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HFR1135496.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            836..1996
                     /gene="pheA"
                     /locus_tag="ACHUQ7_002087"
     CDS             836..1996
                     /gene="pheA"
                     /locus_tag="ACHUQ7_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFR1135497.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2039..3160)
                     /gene="tyrA"
                     /locus_tag="ACHUQ7_002088"
     CDS             complement(2039..3160)
                     /gene="tyrA"
                     /locus_tag="ACHUQ7_002088"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFR1135498.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGKVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3171..4241)
                     /gene="aroF"
                     /locus_tag="ACHUQ7_002089"
     CDS             complement(3171..4241)
                     /gene="aroF"
                     /locus_tag="ACHUQ7_002089"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFR1135499.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4451..4816
                     /locus_tag="ACHUQ7_002090"
     CDS             4451..4816
                     /locus_tag="ACHUQ7_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFR1135500.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKRADENASTIRLN"
     gene            4966..5484
                     /locus_tag="ACHUQ7_002091"
     CDS             4966..5484
                     /locus_tag="ACHUQ7_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFR1135501.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDASSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5474..6699
                     /gene="dgcN"
                     /locus_tag="ACHUQ7_002092"
                     /pseudo
     CDS             5474..6699
                     /gene="dgcN"
                     /locus_tag="ACHUQ7_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6715..7197
                     /locus_tag="ACHUQ7_002093"
     CDS             6715..7197
                     /locus_tag="ACHUQ7_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFR1135502.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVIATP
                     "
     gene            complement(7274..7621)
                     /gene="rplS"
                     /locus_tag="ACHUQ7_002094"
     CDS             complement(7274..7621)
                     /gene="rplS"
                     /locus_tag="ACHUQ7_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFR1135503.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7663..8430)
                     /gene="trmD"
                     /locus_tag="ACHUQ7_002095"
     CDS             complement(7663..8430)
                     /gene="trmD"
                     /locus_tag="ACHUQ7_002095"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFR1135504.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8461..9009)
                     /gene="rimM"
                     /locus_tag="ACHUQ7_002096"
     CDS             complement(8461..9009)
                     /gene="rimM"
                     /locus_tag="ACHUQ7_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFR1135505.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(9028..9276)
                     /gene="rpsP"
                     /locus_tag="ACHUQ7_002097"
     CDS             complement(9028..9276)
                     /gene="rpsP"
                     /locus_tag="ACHUQ7_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFR1135506.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9413..10774)
                     /gene="ffh"
                     /locus_tag="ACHUQ7_002098"
     CDS             complement(9413..10774)
                     /gene="ffh"
                     /locus_tag="ACHUQ7_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFR1135507.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10941..11732
                     /locus_tag="ACHUQ7_002099"
     CDS             10941..11732
                     /locus_tag="ACHUQ7_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFR1135508.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLVICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLMHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            11753..13039
                     /locus_tag="ACHUQ7_002100"
     CDS             11753..13039
                     /locus_tag="ACHUQ7_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFR1135509.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGKYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(13094..13687)
                     /gene="grpE"
                     /locus_tag="ACHUQ7_002101"
     CDS             complement(13094..13687)
                     /gene="grpE"
                     /locus_tag="ACHUQ7_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFR1135510.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13810..14688
                     /gene="nadK"
                     /locus_tag="ACHUQ7_002102"
     CDS             13810..14688
                     /gene="nadK"
                     /locus_tag="ACHUQ7_002102"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFR1135511.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14774..16435
                     /gene="recN"
                     /locus_tag="ACHUQ7_002103"
     CDS             14774..16435
                     /gene="recN"
                     /locus_tag="ACHUQ7_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFR1135512.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16584..16925
                     /gene="bamE"
                     /locus_tag="ACHUQ7_002104"
     CDS             16584..16925
                     /gene="bamE"
                     /locus_tag="ACHUQ7_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFR1135513.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16987..17277)
                     /locus_tag="ACHUQ7_002105"
     CDS             complement(16987..17277)
                     /locus_tag="ACHUQ7_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFR1135514.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17267..17704)
                     /gene="ratA"
                     /locus_tag="ACHUQ7_002106"
     CDS             complement(17267..17704)
                     /gene="ratA"
                     /locus_tag="ACHUQ7_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFR1135515.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17875..18357
                     /gene="smpB"
                     /locus_tag="ACHUQ7_002107"
     CDS             17875..18357
                     /gene="smpB"
                     /locus_tag="ACHUQ7_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFR1135516.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18572..18934
                     /gene="ssrA"
                     /locus_tag="ACHUQ7_002108"
     tmRNA           18572..18934
                     /gene="ssrA"
                     /locus_tag="ACHUQ7_002108"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            19139..20380
                     /locus_tag="ACHUQ7_002109"
     CDS             19139..20380
                     /locus_tag="ACHUQ7_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFR1135517.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQ
                     FRYIRPVTKTRAKKSIGPYPSVTLADARNYRAESRSLLAKQIDPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRLCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANF
                     VMAADRGSMIEGGIKGMKLVG"
BASE COUNT         5300 a         5359 c         4815 g         5049 t
ORIGIN      
        1 cccttgagct gccgtttttt tattctgtta gttgtgaaac tgaagcgatt tagtcactat
       61 cgatctcatc aaatatggct cgctttgaga tattcctcaa gtaaaaaaac acctcttcct
      121 gcgatttctc acaaaaaaga ttcgttgaca aaaagtgaca aaattatgag atttccatca
      181 cacattttga catcaggaac ggtatgctga attcaccaag acgggaagac aagaggtaaa
      241 atttatgaca atgaacatta ccagcaaaca aatggaaatt actccggcca tccgccaaca
      301 tgtcgcagac cgtctcgcca aactggaaaa atggcaaaca catctgatta atccacatat
      361 cattctgtcc aaagagccac aagggtttgt tgctgacgcc acaatcaata cacctaacgg
      421 cgttctggtt gccagtggta aacatgaaga tatgtacacc gcaattaacg aattgatcaa
      481 caagctggaa cggcagctca ataaactgca gcacaaaggc gaagcacgtc gtgccgcaac
      541 atcggtgaaa gacgccaact tcgtcgaaga agttgaagaa gagtagtcct ttatattgag
      601 tgtatcgcca acgcgccttc gggcgcgttt tttgttgaca gcgtgaaaac agtacgggta
      661 ctgtactaaa gtcacttaag gaaacaaaca tgaaacacat accgtttttc ttcgcattct
      721 tttttacctt cccctgaatg ggaggcgttt cgtcgtgtga aacagaatgc gaagacgaac
      781 aataaggcct cccaaatcgg ggggcctttt ttattgataa caaaaaggca acactatgac
      841 atcggaaaac ccgttactgg cgctgcgaga gaaaatcagc gcgctggatg aaaaattatt
      901 agcgttactg gcagaacggc gcgaactggc cgtcgaggtg ggaaaagcca aactgctctc
      961 gcatcgcccg gtacgtgata ttgatcgtga acgcgatttg ctggaaagat taattacgct
     1021 cggtaaagcg caccatctgg acgcccatta cattactcgc ctgttccagc tcatcattga
     1081 agattccgta ttaactcagc aggctttgct ccaacaacat ctcaataaaa ttaatccgca
     1141 ctcagcacgc atcgcttttc tcggccccaa aggttcttat tcccatcttg cggcgcgcca
     1201 gtatgctgcc cgtcactttg agcaattcat tgaaagtggc tgcgccaaat ttgccgatat
     1261 ttttaatcag gtggaaaccg gccaggccga ctatgccgtc gtaccgattg aaaataccag
     1321 ctccggtgcc ataaacgacg tttacgatct gctgcaacat accagcttgt cgattgttgg
     1381 cgagatgacg ttaactatcg accattgttt gttggtctcc ggcactactg atttatccac
     1441 catcaatacg gtctacagcc atccgcagcc attccagcaa tgcagcaaat tccttaatcg
     1501 ttatccgcac tggaagattg aatataccga aagtacgtct gcggcaatgg aaaaggttgc
     1561 acaggcaaaa tcaccgcatg ttgctgcgtt gggaagcgaa gctggcggca ctttgtacgg
     1621 tttgcaggta ctggagcgta ttgaagcaaa tcagcgacaa aacttcaccc gatttgtggt
     1681 gttggcgcgt aaagccatta acgtgtctga tcaggttccg gcgaaaacca cgttgttaat
     1741 ggcgaccggg caacaagccg gtgcgttggt tgaagcgttg ctggtactgc gcaaccacaa
     1801 tctgattatg acccgtctgg aatcacgccc gattcacggt aatccatggg aagagatgtt
     1861 ctatctggat attcaggcta atcttgaatc agcggaaatg caaaaagcat tgaaagagtt
     1921 aggggaaatc acccgttcaa tgaaggtatt gggctgttac ccaagtgaga acgtagtgcc
     1981 tgttgatcca acctgatgaa aaggtgccgg atgatgtgaa tcatccggca ctggattatt
     2041 actggcgatt gtcattcgcc tgacgcaata acacgcggct ttcactctga aaacgctgtg
     2101 cgtaatcgcc gaaccagtgc tccaccttgc ggaaactgtc aataaacgcc tgcttatcgc
     2161 cctgctccag caactcaatc gcctcgccga aacgcttata gtaacgtttg attaacgcca
     2221 gattacgctc tgacgacata atgatgtcgg cataaagctg cggatcctga gcaaacagtc
     2281 gcccgaccat cgccagctca aggcggtaaa tcggcgaaga gagcgccaga agttgctcaa
     2341 gctgaacatt ttcttctgcc aggtgcagcc cgtaagcaaa agtagcaaag tggcgcagag
     2401 cctgaataaa cgccatattc tgatcgtgct cgacagcgct aatacgatgc aaccgagcgc
     2461 cccagacctg aatttgctcc agaaaccatt ggtatgcttc cggcttacgt ccatcacacc
     2521 agaccacaac ttgctttgcc aggctaccgc tgtccgggcc gaacatcggg tgtaacccca
     2581 gtaccgggcc atcgtgcgcc gccagcatgg cctgtaatgg tccatttttc actgatgcca
     2641 gatcaaccag aatacaatct ttcggtaaag gcggtaattt gccgataact tgctcagtaa
     2701 cgtggattgg cacactaaca atcaccattc cggcatcggc aacaatatca gccgctcgat
     2761 cccagtcatg ttgctccaga atccgcacct gataacccga tagtgtcagc atcttctcga
     2821 acaggcgtcc catctgaccg ccaccgccga cgataaccac cggacgcagt gacggacaaa
     2881 gtgttttaaa tcctttgtcg ttttcactgg agtaagattc acgcatcacc cgacgcaaaa
     2941 catcctcaat cagatctggc ggtacaccca gagcttccgc ctctgcacga cgcgaggcca
     3001 acatagatgc ctcgcgctcc ggaacataaa taggcagtcc aaagcggctt ttcaccttgc
     3061 ccacttcagc aaccagttcc agacgcttcg ctaataaatt cagcagcgct ttatcgactt
     3121 catcaatttg atcgcgtaat gcggtcaatt cagcaaccat aataaacctc ttaagccacg
     3181 cgagccgtca gctgcccgtt cagatcctga tgaatttcac gcagcaaggc atcggtcatt
     3241 tcccagctaa tgcaggcatc ggttacggat acaccgtatt tcatttcact gcgcggttgc
     3301 tcagaagact gattgccctc gtggatatta ctttcgatca tcagaccaat aattgagcga
     3361 ttaccatctt tgatttgagc aaccacggat tctgccaccg caggctgacg gcgataatct
     3421 ttattggaat taccgtggct gcaatctacc atcagagacg ggcgcagtcc cgcctgttcc
     3481 atctcttttt cacattgcgc aacatccgca gggctatagt tcggcgcttt accaccgcgc
     3541 aggatcacat gcccgtccgg attcccctga gtttgtagca acgcaacctg ccctgcctgg
     3601 ttaatgccaa caaaacagtg cggctgggcg gcggcgcgca tagcgttaat tgctgttgcc
     3661 agactgccgt cggtgccgtt tttaaaacca accggcatgg aaagcccgga ggccatttca
     3721 cggtgagttt gcgattccgt tgtacgagca ccaattgctg accagctaaa cagatcgccc
     3781 aggtattgcg ggctattcgg atctaacgct tccgtcgcca gtggcagtcc catattcacc
     3841 agctcaagca acaatttacg cgcgatttgc agcccggctt ctacatcaaa agagccatcc
     3901 atatggggat cgttaattaa ccctttccag ccgacagtgg tacggggttt ttcaaaatag
     3961 acgcgcatta ccagatagag gctatcgctg acctctgcgg caagagcttt aaatcgacga
     4021 gcatattcca gagcagtttc cggatcatga atggaacaag gaccacatac taccagcaga
     4081 cgaggatcgc gcccggcgat aatatctgaa atggttttac gcgagtcagc aatctgggct
     4141 tcttgttgca ggctcaatgg aaaagcggcc ttcagttgtt ccggagtcat taaaacctgt
     4201 tcgtcggtaa tatgtacgtt attcagcgcg tctttatgca tgatggcgat cctgtttatg
     4261 ctcgtttgcg atagttgctc ctcagcgagg atgacgtaac gataacacat aaagtaaagt
     4321 tttcaatcca tatttcgtac atttttattt acacaggcaa tttagtcgcg ctttcaaccc
     4381 ttgcttctgt atagataaat ttacatcccc tttaaaaaca atccgctatg ctttgaaaaa
     4441 ggagaaagaa atgatgaaaa agtttatcgc ccccttgttg gctttactgg ttagcggatg
     4501 tcagattgat ccttatactc acgcgccaac cttgaccagc accgactggt atgatgtcgg
     4561 tatggaagat gcgatatcgg gcagcgccat aaaagatgac gatgcattta gcgattcaca
     4621 ggcggatcgc ggtctatacc ttaaaggata tgccgaaggg caaaagaaaa cttgccagac
     4681 cgattttact tatgcccggg gactttccgg taaaagcttt cctgccagct gcaacaatgt
     4741 tgaaaatgcc agccaactgc atgaagtatg gcaaaaaagg gctgatgaaa atgccagcac
     4801 aatacgatta aattaattaa aaccccacaa ataaattaaa cataagattt tactcattgt
     4861 gttttatttc tcacattgat gacggtcgca tgaaaaaatg atattttaat gagcatcctg
     4921 aaaaaatatc gatgcatttc gagcgaagat ggtggggatc cctgaatgcg tttttctcac
     4981 cgactgttct tacttctgat tctcctgctg acgggagcac caatactcgc tcaagagcct
     5041 tctgacgttg cgaaaaacgt gcgtatgatg gtttccggta ttgtcagtta cacccgctgg
     5101 cctgcgctat cagggccacc aaagttatgt atcttttcat cttcacgttt tagtaccgcc
     5161 ctacaagaga atgccgcgac atctttgcct tatctgcctg tcatcattca cactcaacaa
     5221 gaggcgatga tttcaggctg caatggtttt tattttggca acgagtcacc gacattccag
     5281 atggaattaa ccgaacaata tccgtcaaag gcgttattat taattgccga acaaaatacc
     5341 gagtgcatta ttggtagcgc cttttgtctg atcatccaca ataatgacgt cagatttgcc
     5401 gtaaacctgg atgcctcatc gcgtagcggt gtaaaggtca acccggatgt cttaatgctc
     5461 gcacggaaga aaaatgatgg ataacgataa ttctcttaat aagcgcccca cgtttaaaag
     5521 agcattacgc aacatcagta tgaccagcat atttatcact atgatgctga tctggttgct
     5581 gctttccgtg acctcggtgc tgaccctgaa acagtacgcg caaaaaaacc tggcactgac
     5641 agcagcaaca atgacttaca gtctggaagc agctgtcgtt tttgccgatg gccctgcagc
     5701 aactgaaaca ctggcagcgc tgggccagca agggcatttt caactgcaga agtacgtgat
     5761 aagcagcaaa atattctggc atcctggcat tacccccgta aggatccagg cgatactttc
     5821 agcaatttca taagccactg gctcttccct gcccccatca ttcagccgat tcgtcacaat
     5881 ggtgaaacca ttggcgaagt acgcttaacc gctcgcgaca gttcaatcag ccattttatc
     5941 tggttttcgc tcgccgtact gaccggttgt attctgctgg catcaggcat cgcaattacc
     6001 ctcacccgcc atttgcacaa tggcctggtg gaagcactga aaaatatcac cgatgtcgta
     6061 catgatgtgc gttccaaccg caatttttcc cgacgagttt cggaagaacg tatcgctgag
     6121 tttcaccgct tcgctctcga cttcaacagt ctgctggatg aaatggaaga gtggcagctt
     6181 cgtttacagg ccaaaaatgc gcagcttctg cgtaccgcgc tacatgaccc attaaccggg
     6241 ctggctaacc gcgcagcgtt tcgtagcggc atcaatacgt tgatgaacaa ttccgatgcc
     6301 cgaaaaacgt cggcgttact atttcttgat ggcgataatt tcaaatatat caatgatacc
     6361 tggggtcatg cgacgggcga tagggtcttg attgaaatcg caaaacggtt agctgaattt
     6421 ggcgggctgc gacataaagc ataccgcctg ggcggcgatg aattcgctat ggtgctctat
     6481 gatgtacagt cggaatctga agtgcagcag atatgctcag cactgacaca aatctttaat
     6541 ctcccgtttg atcttcataa tggccatcag accaccatga cattaagcat tggttacgcg
     6601 atgaccattg agcacgcctc tgcggaaaaa ttacaagagc ttgccgatca caatatgtat
     6661 caggccaaac accagcgtgc cgaaaagctg gtgagataac aaggatatat cgatatgata
     6721 aagcacctgg tagcacccct gattttcacc tcactaatac tgactggctg ccagtcccct
     6781 cagggaaagt ttactcctga gcaagtcgcc gctatgcaat cttatggatt tactgaatcc
     6841 gccggcgact ggtcgctggg cttatcagat gccattctgt tcgcaaaaaa tgactacaaa
     6901 ttgctcccgg aaagccagca acagatccaa accatggcag ccaaattggc ctcgacaggg
     6961 ctaacacatg cccgtatgga tggacacacc gataactatg gtgaagacag ttacaacgaa
     7021 ggcttatcat tgaaacgggc gaatgtcgtg gccgatgcat gggctattgg tggacaaatt
     7081 ccacgcagca atctcaccac acagggttta ggaaaaaaat atcccatagc cagtaacaag
     7141 accgcccagg gccgcgccga gaaccgccgc gtcgcagtgg tgattgcaac cccttaaaac
     7201 acgagcaaac agacaaaaaa agggccagcc aattggccag cccttcttaa caggatgtcg
     7261 cttaagcgaa atcttagtta agacgctctt tgatacgagc agccttacca gtacgctcac
     7321 gcaggtagta cagtttagct ttacgaacag caccacgacg tttgacagaa atgctgtcaa
     7381 ctaccggaga gtgagtctgg aagacacgct caacgccttc gccgttggaa attttacgaa
     7441 cagtgaatgc agagtgcaga ccgcggttac gaatagcgat aaccacgccc tcgaatgcct
     7501 gcagacgttt tttggaacct tcaacaaccc atactttcac ttccacggta tcacccggac
     7561 ggaaggaagg tacgtcctgc ttcatctgct cttgttcaag ttgcttaata atgttgctca
     7621 taatttaatc tcttatcctg ggtaaactga tctctcgggg gcttacgcca tcccatcatg
     7681 tttatgttgc tgttgtgcgt gttccgtttt gaactccgcc agcaaccttg cttgctcttc
     7741 agtcagagcc aggttttcca gaagttcagg tcttctaagc caggtacggc ccagcgactg
     7801 tttcaaacgc cagcgacgta tctcggcatg gttgcccgac agtaacactg gcggaacttc
     7861 catcccttct aacacctcag gccgcgtata gtgcgggcaa tccagcaatc cttcagcaaa
     7921 ggaatcttcc gttgccgagg cttcatgtcc cagtactccc ggaataaacc gggaaacgga
     7981 gtcaatcagc gtcattgctg gtaactcacc accactgaga acgtaatcgc cgattgacca
     8041 ttcttcgtca atttcggttt ggatcacgcg ctcatctata ccttcgtagc gaccgcacac
     8101 cagaatcaat ttttgattcg ttgccagttc gctgacgccc gcttgatcaa gcttgcgtcc
     8161 ctgtggtgac agataaatca cctttgcgcc ttcacccgcc gcggcttttg ctgcatgaat
     8221 ggcgtcccgc aagggttgca ccatcattaa catccccggt ccgccgccgt aaggacgatc
     8281 gtccacggta cggtgccggt catgcgtgaa gtcgcgagga ctccaactct ggatgctcag
     8341 caggccattt ttaactgccc ggccagttac cccgtaatcg gtaattgcgc ggaacatttc
     8401 aggaaacagg ctaattatgc caatccacat agcgccgcct tttaccgttt atccggtggt
     8461 ttaaaaacca ggatcccaat ctacttcgat tgaacgagta gtgagatcga ctttcttgat
     8521 aacctgccca tcgaggaacg gtacgagacg ttccttgata ccaaacgcat ctttcaggtt
     8581 tgccttaatg acgagaacgt cattagatcc ggtttccatc atatcaacga ctttaccgag
     8641 atcgtagcct tcagtggtta ctacctggca gcccatcagg tctttccagt agtagtcccc
     8701 ctcttcaagc tgaggcagct gcgatgaatc cacgacaatt tcacaattcg tcagcaggtt
     8761 cgccgcatca cgatcgtcaa cgcctttcag cttgatgatc atgtcctgat tgtggtgctt
     8821 ccagctttcc agctggactt gctgccactg acccgccttc tggataaacc agggctgata
     8881 gtcaaaaatg ctttcggcgt cttcggtgga agaaaacact ctgagccacc cacgaatacc
     8941 gtaagacgaa cccatttttc ccaaaacgat gggatcaaca ggtgcttgcg cggtgagttg
     9001 tttgctcatc atgaccaccg tgacagatta agctgctttg tttacttctt tgatcagcgc
     9061 agcaacgcga tcagaaatag ttgcgccctg gccaacccag tgagcgatgc gatccagatc
     9121 caggcgagtg ccttcttctt tttcgctagc gattgggttg aagaaaccaa cgcgctcgat
     9181 gaagcgaccg ttgcgtgcat tacggctgtc agcgacaaca acctggtaga acggacgctt
     9241 tttagcgccg tgacgtgcta aacgaatagt taccataaca tcctcttgtg tgaataaaac
     9301 aaccggaccc catcgaggaa cggagtccgg tgtcatatta aaagcccgaa aattttactg
     9361 atttctggag aaaatgcaat cagcagttaa taactctgtc gcataaggtc atttagcgac
     9421 caggaaatcc cggcggcatc attcccttca tgcttctcat catcttcgcc attccgcctt
     9481 tcttcatttt cttcatcatg cgctgcatgt cgtcgaactg cttcagaagg cggttaacgt
     9541 cctgtacctg catcccgcaa ccggcggcga tacgacgctt acgcgaacct ttgatgattt
     9601 ctggcttagc gcgctctttc atcgtcattg agttgatgat ggcttccata cgcaccagca
     9661 ctttatcgtc catctgcgac ttgacgttat ccgggatctg ccccatgccc ggcagtttgc
     9721 ccatcaagct ggccatgccg cccatatttt tcatctggcg cagttgctca agaaagtcgt
     9781 tgagatcgaa gccgtcgcct ttttttagct tgctggctaa tttctctgcc tgcgcgcggt
     9841 caactttgct ttcgatatct tcgatcagcg acagtacgtc gcccatgccg agaatacgcg
     9901 acgcgatgcg gtccggatgg aacggctcca gcgcctcagt tttctcgcca acaccgagga
     9961 acttgatcgg tttgccagtg atgtgacgaa tagagagcgc cgcaccgccg cgggcatcgc
    10021 cgtccacttt ggtcaacact acgccggtaa gcggtaacgc ttcattgaat gcttttgccg
    10081 tattggccgc atcctgaccg gtcatggcgt caaccacaaa cagggtttca accgggttaa
    10141 tcgacgcatg gacttgtttg atctcgtcca tcatcgcttc gtcaacgtgc agacgaccag
    10201 cggtatccac cagcagcacg tcgtagaatt tcagtttggc ttctttcagc gccgcgttaa
    10261 caatatcaac cggcttctga ccaacatcag aagggaagaa atcaacgccc acctgctctg
    10321 ccagcgtctc aagctgtttg attgccgccg ggcgatagac gtcagcagaa accaccagca
    10381 ctttcttctt gtgcttctcg cgcaggaaat taccgagttt accaacgctg gttgttttac
    10441 cggcaccttg caggcctgcc atcagcacga ccgcaggcgg ttgcgcagcc agattcaggg
    10501 tctggttctc ttcgcccatc gccgcaacca gttcgttacg gacgattttg acgaactcct
    10561 gccccggcgt caggctctta ttaacttcat gaccaaccgc tttctctttt acgcgattga
    10621 taaactcacg cactaccggc agagcgacgt ccgcctccag cagcgccatg cgcacttcgc
    10681 gcagcgtatc ttttacgttg tcttcagtga ggcgtccacg gccactgata ttgcgcagcg
    10741 tgcgcgacaa acgatcggtt aaattatcaa acattgtctc tcgcctgggg tggaaacggt
    10801 tggccgcaat cgcgacacat catcagtatt ttgccgcagt ataacatgaa ggcgtctttg
    10861 ttgttatgca acggttggag cagcgttcac ctgacgctat actgcttctc tttcttattg
    10921 ctcaaactgt cgacattact atgcccgttt ttgctctgct cgcgcttgtc gcctactccg
    10981 tcagtcttgc gctgattgtt cccggtctgc tgcaaaaaaa cggcggctgg cggcgcatgg
    11041 ctattatttc tgcggtcatt gcgctggtct gccacgcaat cgctctggaa gcccgcatcc
    11101 tgcccgacgg tgatagcgga caaaacctca gcctgctgaa cgttggttca ttggtcagtt
    11161 tggtgatctg tacggtaatg accattgtgg cttctcgcaa tcgtggctgg ttgctgctac
    11221 ccattgtcta tgcctttgcg cttatcaacc tggcgctggc gaccttcatg cccaatgaat
    11281 acatcaccca tctggaagca acgcctggga tgctgatgca cattggctta tcgctctttt
    11341 cctatgccac gctaattatc gccgccctgt acgcgctgca actggcgtgg attgattacc
    11401 aactgaagaa caagaagctg gcatttaacc aagaaatgcc gccattgatg agtatcgagc
    11461 gtaaaatgtt ccacatcacg cagattggcg tggtgctgct aacgctcacg ctttgcactg
    11521 gcctgttcta catgcacaac ctatttagca tggaaaatat cgacaaggct gtgctctcta
    11581 tcgtggcgtg gtttgtctat attgtgctgc tgtggggaca ttaccatgaa ggatggcgtg
    11641 gacgccgcgt cgtctggttt aacgttgcgg gcgcggtaat tctgacactg gcctacttcg
    11701 gcagccgaat tgtccagcag ttaatcagct aaacccagaa aaggagtttc ccctggaaca
    11761 catttctact actacgttga tcattattct gatcatcatg gtggtcattt cagcctattt
    11821 ttccgggtcc gaaaccggaa tgatgaccct caaccgctat cgtcttcgac atatggcgaa
    11881 acagggtaat cgctcggcca aacgcgtcga aaaattgctg cgtaagccag accgcctgat
    11941 aagcctggtg ttaatcggca ataacctggt caatattctt gcctccgcgc tcggcactat
    12001 tgtcgggatg cgtttgtacg gcgatgcggg cgtggcaatt gcgactggtg tgctgacttt
    12061 tgtcgtactg gtttttgctg aggtattgcc gaaaaccatt gccgcgctgt acccggaaaa
    12121 agtcgcttat ccgagtagtt ttctgctggc tccgctgcaa attttgatga tgccgctggt
    12181 ctggttgctg aatgctatca cccgtatgct gatgcgcatg atgggtatca aaaccgatat
    12241 cgtggttagc ggctctttga gcaaagaaga gttgcgcact atcgtgcatg aatcgcgctc
    12301 acaaatttcc cgtcgcaatc aggatatgct gctgtcggtg ctcgatctgg aaaaaatgac
    12361 cgttgatgac atcatggtac cgcgcagtga aattatcggt attgatatca acgatgactg
    12421 gaaatctatt ctgcgccaac tctcccactc acctcacggg cgcatcgtgc tttaccgtga
    12481 ttcgctggac gacgccatca gtatgctgcg agtacgtgaa gcctggcggt tgatgtcgga
    12541 gaaaaaagag ttcaccaaag aaaccatgct gcgcgccgcg gacgagatct attttgtgcc
    12601 tgaaggtacg ccgctcagca cgcagttggt aaagtttcag cgcaacaaaa agaaagtcgg
    12661 cctggtcgtc aacgagtatg gagacattca ggggctggtg acggttgaag atattctgga
    12721 agagattgtt ggcgatttca ctacgtcgat gtcgccaaca cttgccgaag aggtcacgcc
    12781 gcaaaacgac ggttcggtga ttatcgatgg caccgccaac gtgcgggaaa tcaacaaagc
    12841 ctttaactgg catctaccgg aagatgatgc ccgcacggtt aatggcgtca ttcttgaggc
    12901 actggaagag atccctgtcg caggcacccg cgtgcgtatt ggcaagtacg atatcgatat
    12961 tctcgacgta caggacaata tgattaagca ggtaaaagtt tttcctgtga aaccgctgcg
    13021 cgagagtgtg gcggagtaac gaaaacggcc ctgcattcgc atgcagggcc gtgaattatt
    13081 acgaaaacag aaattacgct tttgctttcg ctacagttac catcgccgca cgaatcgtac
    13141 gaccattcag cgtataaccc ttctgcataa tgcccagtac gttacctggc gcaacgtcat
    13201 cagattccac cattgcgata gcctgatgca cattcgggtc cagcggcacg ttagtttcgg
    13261 cgatcacttc aacaccaaac ttacgcacaa catccagcat cgacttcagc gtcagctcaa
    13321 tgccttcaac catcgcagac atatccgggt tagctttatc agccacttcc agcgcacgat
    13381 ccaggctatc aatcaccggc agcaattcgt tgatgaattt ctccagcgcg aatttgtggg
    13441 ctttttcaat atccagttca gtacgacgac gcaggttttc catttcggct tttacgcgca
    13501 aaataccgtc acgttcacgg gtctgggctt cagccagctg agcttcgaga ttcgcaattt
    13561 tttcatcgcg cggatccacc tgctcagcag aagcttctgg ctcaactgcc tcaatctctt
    13621 cgtgctgatc catgataatt tcttccgggg cttgcccctc aggcgttttc tgttctttac
    13681 tactcatgaa tttctccgcg tttttttcgc attcatctcg ctaacttcgc ttattatggg
    13741 gatcagtttc agggtttcaa gggaagcact cacattgtca tcaatcttcg caacaaggac
    13801 ctcggaaaaa tgaataatca tttcaagtgt attggcattg tgggacaccc acggcaccct
    13861 actgcactga caacacatga aatgctctac cgctggctgt gcacaaaagg ttacgaggtc
    13921 atcgttgagc aacaaatcgc tcacgaactg caactgaaga atgtgaaaac tggcacgctc
    13981 gcggagattg ggcaactggc tgatctcgcg gtagtcgttg gtggcgacgg taatatgctg
    14041 ggcgcggcgc gcacgctcgc ccgttacgat attaaagtta ttggaatcaa ccgtggcaac
    14101 ctgggtttcc tgactgacct tgaccccgat aacgcccagc aacaattagc cgatgtgctg
    14161 gaaggtcact acatcagcga gaaacgtttt ttgctggaag cgcaagtctg tcagcaagat
    14221 tgccaaaaac gcatcagcac cgcgattaac gaagtggtac ttcatcctgg caaagtggcg
    14281 catatgatag agttcgaagt gtatatcgac gagatctttg cgttttctca gcgatctgat
    14341 gggctaatta tttcgacgcc aacaggctcc accgcctatt ccctctctgc gggcggtcct
    14401 attctgaccc cctctctgga tgcgattacc ctggtgccca tgttcccgca tacgttgtca
    14461 gcacgaccac tggtcataaa cagcagcagc acgatccgtc tgcgtttttc gcatcgccgt
    14521 aacgacctgg aaatcagttg cgacagccag atagcactgc cgattcagga aggtgaagat
    14581 gtcctgattc gtcgctgtga ttaccatctg aatctgattc atccgaaaga ttacagttat
    14641 ttcaatacat taagcaccaa gctcggctgg tcaaaaaaat tattctaatt ttacgccagc
    14701 ctctttactg tatataaaac cagtttatac tgtacacaat aacagtaatg gtttttcata
    14761 caggaaaacg actatgttgg cacaactgac catcagcaac tttgctatcg ttcgtgagct
    14821 tgagattgat tttcatagcg gcatgaccgt aataactggc gagaccggtg cgggtaaatc
    14881 tattgcaata gatgccctcg gtctttgtct cggtggtcgc gctgaagccg acatggtgcg
    14941 taccggcgct gctcgcgctg acctgtgcgc ccgtttttct ctgaaagata cgccagcggc
    15001 cctgcgctgg ctggaagaaa accagcttga agacgggcat gaatgtttgc ttcgtcgcgt
    15061 gatcagcagt gatggtcgct cccgtggttt catcaacggt acagctgttc ctctgtcaca
    15121 actgcgcgaa ctgggtcagt tgctgattca gatccatggt cagcacgctc atcaattact
    15181 caccaaacct gagcaccaaa aattcctgct tgatggctat gccaatgaaa cctctctact
    15241 tcaggaaatg accgcacgtt atcagttgtg gcatcaaagc tgccgtgacc tcgcgcatca
    15301 tcaacagtta agtcaggaac gcgccgcccg tgcggaactg ctgcaatacc aattaaaaga
    15361 acttaacgaa tttaatccgc agcccggaga gtttgagcaa atcgacgaag agtacaaacg
    15421 tctggcgaac agcggtcaat tgctgaccac cagccagaat gcattggcat taatggccga
    15481 cggtgaagac gcaaacctgc aaagtcagct ttacacggct aaacaactgg tgagcgaatt
    15541 gattggcatg gacagcaaac tgtccggcgt acttgatatg ctggaagaag ctaccatcca
    15601 gattgctgaa gccagcgacg aactgcgcca ctactgcgat cgtctggatc tcgatcccaa
    15661 ccgactgttt gaacttgaac agcgcatctc aaaacagatt tcgctggcac gtaaacatca
    15721 cgtcagccct gaggcattac cacagtatta ccagtcgcta ctggaagaac agcagcaact
    15781 ggacgatcag gccgactcac aagaaacgct cgcgctggcg gtaacgaaac atcatcagca
    15841 ggcactggaa accgcgcgcg cattacacca acaacgccag caatatgcag aagaacttgc
    15901 acagctgatc accgacagta tgcatgcgct ctcaatgccg catgggcagt ttacgattga
    15961 tgtcaaatat gacgagcatc acctgggcgc tgacggtgcc gatcgtattg agtttcgggt
    16021 aaccaccaac ccaggtcagc caatgcagcc tattgccaaa gtcgcatccg gtggtgaatt
    16081 gtcccgcatc gcactggcaa tccaggtcat cacggcgcgt aaaatggaaa ccccggcact
    16141 gatttttgat gaagtggatg tagggattag cggtccaaca gcggcagttg tcggcaaact
    16201 gctgcgtcaa ctcggcgaat caactcaggt gatgtgtgtt acccacctgc cacaagtcgc
    16261 gggatgtggt catcaacact attttgtcag caaagaaacc gatggtgcga tgacagaaac
    16321 acatatgcaa tctctggata aaaaagcgcg gttacaagag ctggcgcgcc tgcttggtgg
    16381 cagtgaagtc acacgtaaca cactggcgaa tgcgaaagaa ctgcttgcag cgtaaacttt
    16441 tttcctgctt cacggtcaga gtaaacagca aaacgccgta agaccggaaa gcaaaaggtt
    16501 ttaaagtgat gaaaggtcta ttatcatcgg catattacag atgagccacg tactgctcgg
    16561 gcccgaaaag gaatcaaatc actatgcgct gtaaaacgct gactgctgca gcagcagtac
    16621 tattgatgtt aaccgcaggc tgttccactc tggagcgagt ggtttaccgt cctgacatca
    16681 accaggggaa ctatctgacc gctaacgacg tatccaaaat acgtgttggc atgacgcaac
    16741 aacaagttgc gtacgcattg ggtacaccgc tgatgtccga tccatttggt acgaatacct
    16801 ggttctatgt cttccgccag caaccaggtc atgaaggtgt aacgcagcaa acgctgacgc
    16861 tgacctttaa cagtagcggt gtgttgacca atattgataa caaacctgcg ctgagtggta
    16921 actaataata aagttgctct caaagacgtt aaaaaaggtg ctcaatgagc accttttttc
    16981 tgtctgttat ttattcgctg atttttctgc tcgttgcctg cgaagctctt tcggatcggc
    17041 aatgagaggg cgataaatct ccacccgatc gccatcatgc acactatcgc ttagttttgc
    17101 cggacggctg taaatgccga ctttgttttt agttaaatcg atatcggtac gcaattccag
    17161 caagccactg gcgcgaatag cttcttcaac cgtcgcgccc tcctgcagcg tcactcgctg
    17221 caggtattgc ttctcaggta gcgcataagc cacctcaacg gcaattttac ctagcactgt
    17281 aaacctcttt cgcacgaacc gtaaaagcct ggaccatatt agccgccagc tctttaaaca
    17341 cgcgaccaaa ggcgagttca atcaacttat tggtaaactc aaagtcgaga tgaaactcga
    17401 tacgacacgc ctcctggctc agcggcgtaa acttccatcc accaatcaat ttcttgaacg
    17461 gcccatccac cagattcata agaatacttt ggttactggt caactggttg cgggtagtaa
    17521 acgttttgct gatcccagcc ttagagacat ctaccgccgc agtcatctgc ccaggagtgg
    17581 actccagaat ccgacttccg gtacaacctg gcaaaaactg aggataagac tgaacgtcat
    17641 tcactaactg atacatttgc tccgcgctgt agggtaccag tgcggtccgg ctaatctgag
    17701 gcataacaat ttccatcaac aaaaatccaa caaataatat catttatcct gctgtaaaaa
    17761 aaacgctatc ccggcgctgg gtaacatcgg gttcatgcta agatagagcc ttgtcccccg
    17821 caggattgat atggggtatt ttcgatttca gattaccgat gattcacgac gcttatgacg
    17881 aagaaaaaag cacataaacc tggttcagcg accatcgcgc ttaacaagcg cgcccgtcac
    17941 gaatacttta tcgaagaaga gttcgaagcg ggacttgccc tgcaaggctg ggaagttaaa
    18001 tccctgcgcg caggaaaagc caatatcagc gacagctacg tccttctgcg tgacggagag
    18061 gcatttctgt ttggcgctaa catcacgcca atggccgtgg cctccacgca tgtggtgtgc
    18121 gatcctaccc gtacccgcaa gttacttctc aaccagcgcg aactggactc attgtacggt
    18181 cgcgtcaatc gagaaggcta taccgtagtg gcgctctccc tgtactggaa aaatgcctgg
    18241 tgcaaagtga aaatcggcgt cgccaaaggt aaaaaacagc acgataaacg ttcagatatc
    18301 aaagaacgcg aatggcaggt ggataaagca cgcatcatga aaaacgccca ccgttaaacc
    18361 tgcactccaa ttattgacca gttcctcacc gcgcctccct ctccggcggc gcgaatgaac
    18421 atcttattgg ctatcacatc cgacacaaat gttgccatcc cattgcttaa tcgaataaaa
    18481 atcaggctac atgggtgcta aatctttaac gataacgcca ttgaggctgg tcatggcgct
    18541 cataaatctg gtatacttac ctttacacat tggggctgat tctggattcg acgggatttg
    18601 cgaaacccaa ggtgcatgcc gaggggcggt tggcctcgta aaaagccgca aaaaatagtc
    18661 gcaaacgacg aaaactacgc tttagcagct taataacctg cttagagccc tctctcccta
    18721 gcctccgctc ttaggacggg gatcaagaga ggtcaaaccc aaaagagatc gcgtggaagc
    18781 cctgcctggg gttgaagcgt taaaacttaa tcaggctagt ttgttagtgg cgtgtccgtc
    18841 cgcagctggc aagcgaatgt aaagactgac taagcatgta gtaccgagga tgtaggaatt
    18901 tcggacgcgg gttcaactcc cgccagctcc accaaaattc tccatcggtg attaccagag
    18961 tcatccgatg aagtcctaag agcccgcacg gcgcaagccc tgcgggcttt tttgtgctcc
    19021 gaatttgtcc cgcgaagtct gatgccaact aattaaatcc gatcctttta ggcaccttgt
    19081 taggcacttc ataaagctat attgtctttg aggtgcctaa aactatggaa acccggcaat
    19141 ggcaagacaa accaaacctc tatccgttaa ggaaatcgaa tctgctaaac ccaaggaagc
    19201 ggactacgtt ctctatgatg gcgatggcct tgagctactc attaaatcca gcgggagtaa
    19261 aatctggcag tttcgctaca ttcgccctgt caccaagaca cgagcaaaga aaagcatagg
    19321 cccttacccg tcagttaccc ttgccgatgc cagaaactac cgtgcagagt cccgctcact
    19381 cctagctaaa cagattgatc cgcaggaaca tcagcaagaa caattgcgta gttcgctgga
    19441 agccaaaacc aatactttcc agctcgtagc tgaacgttgg tggaatgtga agaaagccag
    19501 tgtgacagag gactatgcgg aagatatctg gcgctctctt gaaagagatg tctttcctgc
    19561 gattggcgac gttagcgtta cagatattaa agctcataca ctggttcagg ccgtacaacc
    19621 ggttcaggcc agaggagcac tggaaacggt tcgtcgcctg tgtcaacgta ttaatgaggt
    19681 catgatctat gcccagaaca cagggctgat tgatgctgtt cccagcgtta atatcggtaa
    19741 agccttcgaa aagcctcaga aaaagaacat gcccagcatt cgaccggatc agctatctca
    19801 actgatgcag acaatgcgaa cagccagcat tagcctttcc acacgctgcc tgttcatgtg
    19861 gcaacttctt actattaccc gccctgccga agcggctgaa gctcgctggg aagaagtaga
    19921 catagaagcg caagagtgga agattcccgc agcacgcatg aaaatgaacc gcgaccatac
    19981 tgttccattg tcagatgaag caatagctgt gctggagatg atgaaaccgc taagcggcaa
    20041 ccgagaattt atctttccta gccgtatcaa gcccaaccag cctatgaaca gtcagactgt
    20101 taacgcctca ctaaaacgcg caggttttgg tggtgtgctt gtttcgcatg gtctgagatc
    20161 aattgccagt acggctctta atgaacaagg ctttccgcct gatgccattg aggcagcatt
    20221 ggcccatgtg gataagaatg aggttcgtcg tgcttataac cgcagcgatt acctggaaca
    20281 gcgtcgcccg atgatgcaat ggtgggcaaa ttttgtgatg gcagcggatc gtggaagcat
    20341 gattgaaggt gggataaaag gaatgaagct agttgggtga ctaaatagct gcgcggcata
    20401 gtgtcccact ttcagccagc ccttgcaggt taagccacca gacagtaaac ggcgggtgtc
    20461 gcgttattag gcgcagctat ttagtagtaa tattaggatt ggtgattctg cactccagaa
    20521 gtt
//
LOCUS       DAZPCS010000059        20520 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZPCS010000059 DAZPCS010000000
VERSION     DAZPCS010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20520
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.059"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            1..2264
                     /locus_tag="ACHUQ7_002110"
                     /pseudo
     CDS             1..2264
                     /locus_tag="ACHUQ7_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
     gene            2265..3914
                     /locus_tag="ACHUQ7_002111"
     CDS             2265..3914
                     /locus_tag="ACHUQ7_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HFR1135518.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QELIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            3919..4695
                     /locus_tag="ACHUQ7_002112"
     CDS             3919..4695
                     /locus_tag="ACHUQ7_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFR1135519.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            4970..7819
                     /gene="rcsC"
                     /locus_tag="ACHUQ7_002113"
     CDS             4970..7819
                     /gene="rcsC"
                     /locus_tag="ACHUQ7_002113"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFR1135520.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPCVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            complement(8019..8669)
                     /gene="rcsB"
                     /locus_tag="ACHUQ7_002114"
     CDS             complement(8019..8669)
                     /gene="rcsB"
                     /locus_tag="ACHUQ7_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFR1135521.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(8686..11358)
                     /gene="rcsD"
                     /locus_tag="ACHUQ7_002115"
     CDS             complement(8686..11358)
                     /gene="rcsD"
                     /locus_tag="ACHUQ7_002115"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFR1135522.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTRQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            12096..13217
                     /gene="ompC"
                     /locus_tag="ACHUQ7_002116"
     CDS             12096..13217
                     /gene="ompC"
                     /locus_tag="ACHUQ7_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFR1135523.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            13329..14384
                     /gene="apbE"
                     /locus_tag="ACHUQ7_002117"
     CDS             13329..14384
                     /gene="apbE"
                     /locus_tag="ACHUQ7_002117"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFR1135524.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            14458..15522
                     /gene="ada"
                     /locus_tag="ACHUQ7_002118"
     CDS             14458..15522
                     /gene="ada"
                     /locus_tag="ACHUQ7_002118"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFR1135525.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            15522..16172
                     /gene="alkB"
                     /locus_tag="ACHUQ7_002119"
     CDS             15522..16172
                     /gene="alkB"
                     /locus_tag="ACHUQ7_002119"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFR1135526.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVAPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            16248..17891
                     /gene="yojI"
                     /locus_tag="ACHUQ7_002120"
     CDS             16248..17891
                     /gene="yojI"
                     /locus_tag="ACHUQ7_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFR1135527.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAVDQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            18109..18258
                     /locus_tag="ACHUQ7_002121"
     CDS             18109..18258
                     /locus_tag="ACHUQ7_002121"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1135528.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTY"
     gene            18367..19733
                     /gene="mqo"
                     /locus_tag="ACHUQ7_002122"
                     /pseudo
     CDS             18367..19733
                     /gene="mqo"
                     /locus_tag="ACHUQ7_002122"
                     /inference="COORDINATES: protein motif:HMM:NF017817.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
     gene            complement(19855..20364)
                     /gene="eco"
                     /locus_tag="ACHUQ7_002123"
     CDS             complement(19855..20364)
                     /gene="eco"
                     /locus_tag="ACHUQ7_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFR1135529.1"
                     /translation="MKKESKKMKTILPEVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     TGMKRQVIQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAEVR"
BASE COUNT         4976 a         4989 c         5511 g         5044 t
ORIGIN      
        1 attaacctcg tcgctgaaag gtaaacctgt ttctgcgcag ctaacggtag gcgtggtcga
       61 tgaaatgatc tacgcgctgc aaccagaaat cgcgccgaat atcggcaaat ttttctatcc
      121 gctggggcgt aacaatgtgc gtaccagctc cagtctgtcg tttatcagct atgaccaggc
      181 gctctccagc gagccggttg cgcctggcgc gactaaccgc agcgagcggc gagtaaaaat
      241 gcttgaacgt ccacggcgtg aagaggtgga taccgcggca tggatgccgt cactcacaac
      301 cgataaacaa ggcaaagcgt atttcacgtt cctgatgcct gattcgttaa cccgctggcg
      361 tatcaccgcg cgtgggatga acggcgacgg gctggtcggg caggggcgtg cttatctgcg
      421 ttcggaaaaa aatctctaca tgaagtggag tatgccaacg gtgtatcgcg tgggcgacaa
      481 accggcggca ggactgttta tcttcagtca gcaggataac gaaccggtgg cgctggtgac
      541 taaatttgca ggcgctgaga tgcgccagac gctgacgctg cacaaagggg cgaattatat
      601 ttcgctgacg cagaatattc agcaatctgg cttgttaagt gcagaactgc aacaaaatgg
      661 gcaagtgcag gacagcatta gcacaaaact gtcttttgtg gacaacagct ggcccgttga
      721 acagcagaaa aatgtcatgc tcggcggtgg cgagaacgcg ctgatgttgc ccgagcaggc
      781 gagtaatatc cggctacaaa gtagtgaaac gccgcaggag attttccgca acaatcttga
      841 tgcgttagtc gatgaaccgt ggggtggggt gatcaacacc ggtagccgtc tgatcccgct
      901 cagtctcgcc tggcgttcgc ttgccgatca tcaaagtgcc gccgctaacg acattcgtca
      961 gatgattcag gataaccgtc tgcggctgat gcaactggcg gggcccggag cgcgctttac
     1021 ctggtggggt gaagatggca atggtgacgc cttccttacg gcatgggcat ggtacgccga
     1081 ctggcaggcc agccaggcgc tcggcgtaac gcaacaaccg gaatactggc agcatatgct
     1141 cgacagttat gccgagcagg cggataatat gccgttattg catcgggcgc tggtgctggc
     1201 gtgggcacag gagatgaatc tgccgtgcaa aacgttgttg aaaggggggg atgaagctat
     1261 cgcccggcgc ggaactaaag atgaagattt ctctgaggaa gacatccgcg atatcaatga
     1321 cagcctgatc ctcgatacac cggaatctcc actggcagat gcggtggcaa acgtcttaac
     1381 catgacgttg ctgaaaaaag cgcagttgaa gtccacggtg atgccacagg ttcagcaata
     1441 tgcgtgggat aaagcggcaa acagcaatca gccgctggcg cacacggttg tgctgctcaa
     1501 tagcggtggt gacgctaccc agacggccgc tattttaatg gtttgaccgc tgagcaatcc
     1561 actattgagc gcgcgctggc catgaactgg ctggcgaaat atatggcgac aatgcctcca
     1621 gttgttttgc ctgcgcctgc gggcgcatgg gctaaacata agttaactgg agggggcgaa
     1681 gactggcgtt gggttggtta gggtgtgccg gacattctct cttttggtga cgaattatcg
     1741 ccgcaaatgt gcaggtccgc tggcgtgaac cggcaaaaac ggctcaacaa agtaacattc
     1801 cggtgaccgt tgaacgccag ttgtatcggc ttatccccgg tgaagaagag atgagcttta
     1861 ctctgcagcc ggtgaccagc aatgagattg acagcgatgc gctgtatctc gatgaaatta
     1921 cgcttaccag cgagcaggat gcagttctgc gctatggtca ggtggaagta ccgctcccgc
     1981 cgggagctga cgttgagcgc acaacatggg gcatttcagt caataaaccc aacgccgcga
     2041 aacagcaggg gcaattgctg gaaaaagcgc gtaatgaaat gggcgaactg gcctatatgg
     2101 tgccggtgaa agaactgacg ggaacggtca ctttccgcca tttgctgcgc ttctcgcaaa
     2161 aagggcaatt cgttctgcct cctgctcgtt atgtgcgttc ctatgcacct gcgcagcaaa
     2221 gtgtcgcggc agggagtgaa tggaccggga tgcaggtgaa ataagtgaac tggcgcagaa
     2281 tcgtctggct attggcgttg gttactctgc caacgcttgc ggaggagacg ccgttacaac
     2341 tggtgctgcg cggtgcgcag cacgatcaac tgtatcagct gtcgtcatca ggcgtgacca
     2401 aagtctcagc attacctgac tcgctcacca cgccgttagg gagtttgtgg aaactgtatg
     2461 tttacgcctg gctggaggat acccatcaac cggagcagcc ctaccagtgt cgcggaaact
     2521 ccccggaaga ggtttattgt tgtcaggctg gggagagcat aacgcgcgat accgcgctgg
     2581 tacgttcctg tgggctctat tttgcgccgc agcggttgca tattggcgca gatgtgtggg
     2641 ggcaatactg gcaacagcgt caggcacctg cgtggttagc gtctcttaca acgctgaagc
     2701 cagaaacctc ggtaacggtt aaatcgctgc ttgattcatt agcgacgtta cctgcgcaaa
     2761 acaaagctca ggaagtctta ctggatgtgg tgctggacga ggcgaaaatc ggtgtcgcat
     2821 ccatgctcgg tagtcgggta cgggtgaaaa cctggagctg gtttgccgat gataaacagg
     2881 aaatccgtca gggtggtttt gctggctggt taaccgatgg tacaccgctg tgggttactg
     2941 gcagtggcac cagcaaaacg gtactaaccc gttatgcgac ggtgcttaat cgggttttgc
     3001 cagtgccgac gcaggttgcc agtgggcaat gtgtagaggt ggagctgttt gcccgctatc
     3061 cgctgaagaa aatcacagcg gaaaagagta cgacggcagt aaaacccagc gtacttaatg
     3121 ggcgctatcg tgtgacgttt actaatggta accacattac gtttgtcagc cacggtgaga
     3181 cgaccttact gagcgaaaag ggcaaactga aattgcagtc gcatctcgat cgtgaagagt
     3241 acgttgccag agttctggat cgcgaggcga aaagtacgcc acctgaggca gcgaaagcca
     3301 tgactgtggc gatacgtact tttttacagc aaaacgccaa tcgggaaggt gattgcctga
     3361 ccatccccga cagcagcgcc acgcagcgtg tttctgcttc gccagcgacc acgggggcgc
     3421 gaacgatgac cgcctggacg caggagctca tctatgcagg cgatccggtt cattatcatg
     3481 gcagtcgcgc taccgaaggg acactttcct ggcggcaagc cacggcccag gcggggcagg
     3541 gggagcgtta cgaccagata ctcgcgtttg cttatcccga caacagcctt agccgctggg
     3601 gagctccacg ctcaacctgc cagttattac ccaaagcgaa agcctggctg gcgaaaaaaa
     3661 tgccgcagtg gcggcgtata ttacaaggcg agacggggta caacgaacca gacgtgtttg
     3721 cggtctgtcg tttagtctca ggtttcccct ataccgatcg gcagcagaaa cggctgttta
     3781 ttcgtaactt ctttacgctt caggatcggc tggatttaac ccatgagtat ctgcacttgg
     3841 cctttgatgg ttatcccacc gggcttgatg agaactatat cgaaacgctg acccgacaat
     3901 tattaatgga ctaatgctat gcgaaagatt tttcttccgt tgttactggt ggcactttcg
     3961 cctgtcgctc acagtgaagg tgtgcaggaa gtcgagattg acgcaccgct ctctggctgg
     4021 catccggcag agggcgaaga tgccagtttt tcacaatcga tcaattaccc ggcgtcgtcc
     4081 gtcaacatgg cagatgatca aaatatctca gcgcagatcc gcggcaaaat aaagaattat
     4141 gctgcggcgg gtaaagttca gcagggccgg ctggtggtca acggtgccag tatgccgcag
     4201 agaattgaat ccgatggttc atttgcacgc ccttatattt tcactgaagg cagcaacagc
     4261 gtgcaggtca tcagcccgga tgggcaaagc cggcaaaaaa tgcagtttta ctcaacgccg
     4321 ggtacaggaa ctattcgtgc acgtttacgg ctggttctct cgtgggatac ggacaatacc
     4381 gatctcgatc tccatgttgt cacgcccgat ggcgaacacg cctggtacgg taacaccgtg
     4441 ctgaaaaaca gtggtgcact ggatatggat gtcacgacgg ggtacggacc cgagattttc
     4501 gccatgccag caccagttca cgggcgctat caggtgtata tcaactacta tggtgggcgc
     4561 agcgaaacgg aattgacgac cgcccaactg acgctgatca ccgatgaagg atcggtcaat
     4621 gagaaacagg aaacatttat tgtaccgatg cgtaatgctg gcgagctgac gctggtgaaa
     4681 agttttgact ggtgatccat cccttcacac aacctggctc tctctttttt cagataagac
     4741 tgctgggcga tctgccgacg ccaggatata accattaaca agcgtcggca gattggtcat
     4801 ttcttatttt tcgtactgag aactgtccat ctactccggc tttcctggtt aaggcaatgg
     4861 tttctaagcg atacgcatta ttgcaatggt gacagaacat tgcggtaaca cgcttttacc
     4921 gctaccttaa ccacactcca tcggtcacct gaggcggagc ttctcccctt tgaaatacct
     4981 tgcttctttt cgtacaaccc tgaaagcctc gcgctacatg ttcagagcat tggcgttagt
     5041 gctctggctg ttgattgctt tttcatccgt tttttacatc gttaatgcgt tacatcagcg
     5101 agaatcggaa attcgtcagg aatttaatct gagttccgat caggctcagc gctttattca
     5161 acgcacctct gatgtgatga aagagcttaa gtacatcgcc gaaaatcgct tatcggcaga
     5221 aaacggtgtg ctttccccgc gtggacgaga aacgcaggcg gatgtgcctg cgtttgaacc
     5281 gctgtttgcc gactccgatt gttccgcaat gagtaacacc tggcgaggtt ctctggagtc
     5341 attggcgtgg tttatgcgct actggcgcga taatttttct gcggcttacg atctcaaccg
     5401 ggtattttta atcggcagtg ataacctctg catggccaat ttcggtctgc gtgatatgcc
     5461 agtggaacgc gataccgcgt tgaaagcgtt gcatgaacgc atcaataaat atcgaaatgc
     5521 accacaagat gatagcggca gtaacctcta ctggatcagc gaaggtccgc gcccttgcgt
     5581 cgggtatttt tacgcgttga cgccagttta tctggcgaac cggttgcagg cgcttttggg
     5641 tgtcgagcag accatccgga tggagaactt tttcttaccg ggtacgttgc cgatgggggt
     5701 taccattctt gatgaaaatg gtcataccct gatttcgctt accggaccag aaagtaaaat
     5761 taagggcgat cctcgctgga tgcaggaacg ctcctggttt ggctatacgg aagggttccg
     5821 ggagctggtg ctgaagaaaa atctgccacc ctcatcgcta agcatcgtgt attcggtgcc
     5881 ggttgataag gtgctggaac gcattcgcat gttgatcctt aacgcaattt tgctgaatgt
     5941 gcttgccgga gctgcattgt ttactctcgc gcggatgtac gagcgacgta ttttcattcc
     6001 ggcggaaagc gacgccctgc gactggaaga acatgagcag ttcaatcgca agattgtcgc
     6061 ctccgcgcca gtgggtatct gcattttgcg taccgctgat ggcgtcaata ttttaagtaa
     6121 cgaactggcg catacctatc tcaatatgct tacgcatgag gaccgccaac gactgacgca
     6181 aattatctgt gggcagcagg tcaattttgt tgatgtcctg accagcaaca ataccaatct
     6241 gcaaatcagc ttcgtccatt cgcgctatcg taatgaaaac gtggccattt gtgtgctggt
     6301 ggatgtttct tcgcgcgtga agatggaaga gtcgttgcag gagatggcac aagcagcgga
     6361 acaggcgagc cagtcaaaat cgatgttcct tgccaccgtc agtcatgagc tgcgaacgcc
     6421 gctgtatggc attatcggta acctggatct gttgcaaacc aaagagttac cgaaaggcgt
     6481 cgatcgtctg gtgacggcaa tgaacaactc ttccagcctg ttgttgaaaa ttatcagcga
     6541 tattctcgat ttctcgaaga ttgaatcgga acagttgaag atcgaaccgc gtgagttttc
     6601 accgcgtgaa gtgatgaacc acatcaccgc caactattta ccgctggtgg tacgcaagca
     6661 gttaggcttg tactgcttta ttgaaccgga tgtgccagtg gccttaaatg gcgacccgat
     6721 gcgtttacag caggtcatct ccaacctgtt gagtaacgcc ataaaattca ccgataccgg
     6781 ctgtatagtt ttgcatgttc gcgctgatgg cgattatctc tctatccgtg ttcgcgatac
     6841 cggcgtgggg attccggcga aagaagtggt gcgcttgttt gatcccttct tccaggttgg
     6901 aacgggcgta cagcgaaact tccaggggac cggtctgggt ctggcgattt gtgaaaaact
     6961 gatcagcatg atggacggcg atatctcggt agattcagaa ccgggaatgg gcagccagtt
     7021 taccgtgcgt attccgttgt acggcgctca gtacccgcag aaaaaaggcg tggaagggtt
     7081 gagtggtaaa cgctgctggc tggcggtccg caatgcgtcg ctctgtcagt tcctggaaac
     7141 cagtttgcag cgcagcggca tcgtcgttac aacatacgaa gggcaggaac cgactcccga
     7201 agatgtgtta atcactgacg aggtagtgag taaaaaatgg cagggcagag cggtagtgac
     7261 cttctgtcgt cgccatattg gtattccgct ggagaaagcg ccaggggagt gggtacacag
     7321 tgtggctgct ccgcatgagc taccggcatt gttggcgcgt atttatttga tcgagatgga
     7381 gagcgacgat cctgctaacg ctctgccgtc gacggacaaa gcggtcagcg ataacgacga
     7441 tatgatgatt ctggtcgtgg atgatcatcc gattaaccga cgtctgctgg cagatcagtt
     7501 gggatcgttg ggctatcaat gtaaaaccgc gaatgatggc gtcgatgcgc ttaatgtact
     7561 tagtaagaat catattgata tcgtgcttag cgacgtcaac atgccaaata tggatggtta
     7621 ccgcttgacg caacgcattc gtcagttggg actgacgttg ccggtaatcg gagtaactgc
     7681 taatgcgttg gctgaagaga agcagcggtg tctggagtcc ggtatggaca gctgcctctc
     7741 gaagccggta acgctggatg tgataaaaca gacgctgacg gtatatgccg agagggtcag
     7801 gaaatcgcgg gattcgtagg acggataagg cgtttacgcc gcatccggca ttcagtgcaa
     7861 atgccagata agacactgac gcgtcttatc tggcctactt taatgtaatc tgttgaaata
     7921 atgggaatcg taggccggat aaggcgttta cgccgcatcc ggcaatcggt gcaaatgcca
     7981 gataagacac taacgcgtct tatctggcct acagatgatt agtctttatc tgccggactt
     8041 aaggtcacag aagagagata attcagcagg gcgatatcgt tctcgacgcc cagcttcatc
     8101 atcgcagatt tcttctggct actgatggtt ttaatactgc ggttcagctt tttagcgatc
     8161 tcggtcacta ggaagccttc cgcaaacagg cgcagaactt cactctcttt tggcgagaga
     8221 cgcttgtcac cgtaaccacc agcactgatt ttttccaaca ggcgagaaac gctttccggg
     8281 gtaaatttct tccctttctg cagcgcggcg agagctttcg gcagatcggt cggtgcacct
     8341 tgtttcagca cgatcccttc gatatccaga tccaataccg cactaagaat cgccgggttg
     8401 ttgttcatag tcagaacaat gatcgacagg cttgggaaat ggcgcttgat gtacttgatt
     8461 aaggtaatgc catcgccgta cttatcgcca ggcatggaga gatcggtaat caacacatgc
     8521 gcatccagtt tcggcaggtt gttgatcagt gctgtagagt cttcaaattc gccgacaaca
     8581 ttcacccact caatttgctc aagtgatttg cgaataccga acaagactat cggatggtca
     8641 tcggcaataa ttacgttcat attgttcatg taataggcta ccttgctaca gcaagctctt
     8701 gacataactg tcaatgtcgc tgatgtattt ttctattcct ggaacatcct tctcacgaat
     8761 cagatgttcc agcgtttcac ataactgctt gccgggtacc agatttagca tggcaaatac
     8821 gcctttaaga cgatgagccg tttgggctaa cgcagcaaag tcactggttg ctgcttcagt
     8881 atacagcctc ttaacatcat ccggtactgt gtctacaaag agcgcataat agccgctggc
     8941 atggagttgc gcattttcat ctccgcccag aggcgattct gtcacctctt cctgtgccag
     9001 ttgcacttca attaattgta agaccgcttc ctgcatagcg ttgctcatat tgaagttgac
     9061 gcacaattga ccaggcccaa tttctcgtac gccagactca tcatcgctta aaagcaagcc
     9121 agaggcagta agattagacg gattatccgt taaaaagata tcataatctt gactaattaa
     9181 tctttcatcg ggtgtgatac aggttgcacc ccaattttct aacaggcgag tgacaatatt
     9241 ccgaatttct gccgaagtaa catccaccat tacgcagaca tcatccagta aacgctcttc
     9301 ttcctcttca acttccgggt cagcggcgag cattttgata tgcacagagt agcgtgtacc
     9361 aagcccatcc cgcgttttga tgtttaaatg accgcccagt ttacgtgcca gttgatcgct
     9421 cagccagaat gccagcgggt ccgcctttcc ataacgatcg ttttgggtct ggttgataaa
     9481 cgggaagtgc aaattatcca tttcatgaat acttacgcct tctcccgtgt ccagaatgcg
     9541 gaatgtcagg cggtcttcag aggactcatc ctgatcaacc tcaagggtga tttttcccaa
     9601 ttgcgttgag gtcacggcat attgcatcag tagcagcaaa atacgtcgta aggcatcgcg
     9661 atcgccgcgg cgcatatcgt gtgctttcag atgattgtta atcagcagtt gcagaccttt
     9721 acgcttgatg gcaggcaaca ctgaaggcac aacttcatca attaaatcct gcacggagaa
     9781 cagcaccgtc tcacttttcc agctatcatc cgcaagcatg ttcgctaact gtatttcttc
     9841 gaccagccgc accagcacat ctgcctgatt cgccagttgt ttgctttccg gggcgttgag
     9901 tttagccgca ctctccgcca gggactgtgc gggttctttc agcgcatcgc caatgttttt
     9961 cataaaggtc atccgcccct gctggttttt ctcatacaga cgctgcgcct gcttgagttt
    10021 cttgtttacc agcacttcgc gatcctgatc gcgaataatg aaaatttgtg tgcgcggcgc
    10081 gacctggctg cggaacatgc ggatctcata cagctcgtta ttgatcgtcg cctgaataat
    10141 cccctgatgc tgttccgcca tggtggtgat gttttgcaga ttcagatgcg gcagcaaatg
    10201 atcggcaatt ttgttactta tgacagtgcg gttcgattcc tgatcgtgaa ccagcaggcc
    10261 gagcggcaac agtgagacta tctcttcatt gattgcccgt aaaatgcgca attcgttatt
    10321 gaccgccgtg ctggggacgc tttctgtact gcggctggag aaatggcgga atgtggtata
    10381 gccaaataac gccagcgcca gcaaaccgat gttcagcagc agtggcagca gaatgttttg
    10441 caacgtatcc agcaataagg tgccataagg aacctgccag accaggcgca tatcggtaga
    10501 gttgagtgcc gaggagattt caatcttcgt actgttaaag tggatactga cgctatccgt
    10561 cccttctttc tcattatcat tgtttcccgt tgccgtcgcg tctggctcaa ggcggaaact
    10621 gtccagcggc atacccggtg ggatcaaatc attaatcggc agatcaaaag ccacgaccgt
    10681 tgccagatgt cccggctggt tgaacgtggt acgcaaggta aagtaatgac cgttctgcca
    10741 ggctaggcgg cgcatgttag aaaaactttc gcgttcatcg agggcgttgg cctgttgtaa
    10801 catttctgca cgacgtgaat caacaatgtc actgacggtc gattctttaa atccggaggt
    10861 gagatcttta aggggtaggg ttgagatcag caccagactg ttatcctgac cattcaggta
    10921 atacatcgac cacggtacat tttctgcacc ccacaatgta tccagataag tggacatccg
    10981 ctgagtcatc tcaagcgttg agttgtcgtg agagccaaag attaacgctt ccgttttgcg
    11041 gcgtggtttc tccagatagt agacatcctg tttcaggcgc gtctcttgta aaccttcgcc
    11101 ggaggagggg gaggtcgtcg cggcaatgtt gtcgtagatc tgcctggtca cgtaacgcca
    11161 ggtatcgacg cgcttttgga ttgcgtgggt aatgtcgaca atctggtaac ttttatcttt
    11221 cagccaagcg ttaacggcac tttgtaccat tacccccatc gtcaccagta acacaatgat
    11281 caacagtaaa aagaagcggg taatgctccc cggtaggagt gaaaagcggg tcgtggccgt
    11341 tgtctctttc tgacgcattc gtgtttatga cctgttaaaa cttcgcgaag tgatagggca
    11401 gtataaaggg tacagtgtga aattccagac tcttacctga ttgatgattc tctattctcc
    11461 cgtgatattt atcatcctgt atggggggaa atagatgtac aagcgccatt ttgctgtaca
    11521 aataacctac aaaaagccca acgaaattaa ttgcaaataa acaaagattg ctggaaatta
    11581 tgctgatgtt aattatttgt gaaatagtta acaagcgtta tagtttttct gtggtagcac
    11641 agaataatga aaagtgtgta aagaagggta aaaaaaccga atgcgaggca tccggttgaa
    11701 ataggggtaa acagacattc agaaatgaat gacggtaata aataaagtta atgatgatag
    11761 cgggagttat tctagttgcg agtgaaggtt ttgttttgac attcagtgct gtcaaatact
    11821 taagaataag ttattgattt taaccttgaa ttattattgc ttgatgttag gtgcttattt
    11881 cgccattccg caataatctt aaaaagttcc cttgtattta cattttgaaa catctatagc
    11941 gataaatgaa acatcttaaa agttttagta tcatattcgt gttggattat tctgcatttt
    12001 tggggagaat ggacttgccg actgattaat gagggttaat cagtatgcag tggcataaaa
    12061 aagcaaataa aggcatataa cagagggtta ataacatgaa agttaaagta ctgtccctcc
    12121 tggtcccagc tctgctggta gcaggcgcag caaacgctgc tgaagtttac aacaaagacg
    12181 gcaacaaatt agatctgtac ggtaaagtag acggcctgca ctatttctct gacgacaagt
    12241 ctgtagatgg cgaccagacc tacatgcgtc ttggcttcaa aggtgaaact caggttactg
    12301 accagctgac cggttacggc cagtgggaat atcagatcca gggcaacagc gctgaaaacg
    12361 aaaacaactc ctggacccgt gtggcattcg caggtctgaa attccaggat gtaggttctt
    12421 tcgactacgg tcgtaactac ggcgtagttt acgacgtaac ttcctggacc gacgtactgc
    12481 cagaattcgg tggtgacact tacggttctg acaacttcat gcagcagcgt ggtaacggct
    12541 tcgcaaccta ccgtagcacc gacttctttg gtctggttga cggtctgaac ttcgctgttc
    12601 agtatcaggg caaaaacggt agcccggaag gtgaaggcat gaccaacaac ggtcgtgaag
    12661 ctctgcgtca gaacggtgac ggcgttggcg gttctattac ttatgattac gaaggcttcg
    12721 gtatcggtgc tgcagtttcc agctccaaac gtactgatga ccagaacttc ggtctgaacc
    12781 gttatgatga gcgttacatc ggtaatggcg accgcgctga aacctacacc ggtggtctga
    12841 aatacgacgc gaacaacatc tacctagctg ctcagtacac ccagacctac aacgcaactc
    12901 gcgtaggcaa cttgggttgg gcgaacaaag cacagaactt cgaagctgtt gctcagtacc
    12961 agttcgactt cggtctgcgt ccgtccctgg cttacctgca gtctaaaggt aaaaacctgg
    13021 gtgtcatcaa tggtcgtaac tacgacgacg aagatatcct gaaatatgtt gatgttggtg
    13081 cgacctacta cttcaacaaa aacatgtcca cctacgttga ctacaaaatc aacctgctgg
    13141 acgacaacca gttcactcgt gacgctggca tcaacactga taacatcgta gctctgggtc
    13201 tggtttacca gttctaatct cgatggatat cgaacaaagg gcctgcgggc ccttttttca
    13261 ttgttttcag cgtacaaaca cagttttttg gtgtactctt gcgaccgttc gcatgaggat
    13321 aatcacgtat ggaaataagc tttacccgcg tggcactgct ggttgccgcg ctcttctttg
    13381 ttggttgcga tcaaaaacca caacccgcca aaacccacgc tactgaagtt accgttcttg
    13441 aaggcaaaac catgggtacc ttctggcgtg ccagcatccc gggcattgat gccaaacgca
    13501 gtgccgaact taaagaaaag attcagaccc agctggacgc cgacgatcag ctgctttcga
    13561 cctataaaaa agattccgcg ctgatgcgct ttaacgactc gcaaagtttg tcgccgtggc
    13621 cggtaagtga agcgatggcc gatatcgtca ccacctcgct gcgcattggg gcaaggaccg
    13681 atggtgcgat ggatattacc gtcgggccgc tggtgaatct gtggggcttt ggcccggaac
    13741 aacagccggt tcaaattccg agccaggaac agatcgatgc gatgaaagcc aaaaccggct
    13801 tacagcacct gacggtcatc aatcagtcgc atcagcaata tctgcaaaaa gacctgccgg
    13861 atttatatgt cgatctctcc accgtcggtg aaggttatgc ggcggatcac ctggcacgct
    13921 tgatggagca ggaagggatt tcccgctatc tggtgtcggt gggcggcgcg ctgaacagcc
    13981 gtggtatgaa cggtgaaggc cagccgtggc gggtagcgat tcaaaaacca accgataaag
    14041 aaaacgcggt tcaggctgtg gtggatatca acggtcatgg gattagcacc tctggcagct
    14101 accgtaacta ttacgaactg gacggcaaac gtctttccca tgttatcgat ccgcaaaccg
    14161 ggcgtcccat cgaacacaat ctggtatccg tgacggtgat tgctccgacg gcgctggaag
    14221 ccgatgcctg ggatactggc ttgatggtac tcgggccgga gaaagccaaa gaggttgttc
    14281 gccgggaagg gctggcggtt tatatgatca ccaaagaagg cgatagcttt aaaacctgga
    14341 tgtcaccaca gtttaaaagc ttccttgtca gcgaaaaaaa ttaaagcgca agattgttgg
    14401 tttttgcgtg atggtgaccg ggcagactaa aggctatcct taaccaggga gctgattatg
    14461 aaaaacgcca catgcttaac tgacgatcaa cgctggcaat ctgtcttagc ccgcgacccg
    14521 aatgccgacg gcgaattcgt tttcgccgtg cgcaccacgg gtatcttttg ccgtccttct
    14581 tgccgcgcca gacatgcctt gcgggaaaat gtctccttct acgcaaatgc cagcgaggca
    14641 ctcgccgccg gatttcgccc ctgcaaacgt tgtcagccag acaaagccaa tccccggcaa
    14701 catcggttgg ataaaatcac ccacgcgtgt cgactgctgg aacaggaaac gcctgtaacg
    14761 ctggaagcct tagccgacca ggtggcgatg agtccatttc atctacatcg gttgtttaaa
    14821 gctactaccg gaatgacgcc taaagcctgg caacaggcct ggcgcgctcg ccgtttgcgc
    14881 gaatcgctgg cgaaagggga gagcgtgacg acgtctattc ttaacgccgg attccccgac
    14941 agcagcagtt actaccgcaa agccgatgaa acgctgggca tgacggctaa acaattccgt
    15001 cacggtggcg aaaatctggc ggtgcgttac gcgctggctg attgtgagct gggtcgttgc
    15061 ctggtggcag aaagcgagcg ggggatttgc gcgatattgc tgggcgatga tgacgcgaca
    15121 ttaatcagcg agttgcagca gatgtttccc gctgccgaca acgcgcctgc cgatctgacg
    15181 tttcagcaac atgtgcgtga agtgatcgcc agcctcaatc aacgcgatac gccgctgacg
    15241 ttaccgctgg atattcgcgg cactgctttt cagcaacaag tctggcaggc actgcgcacg
    15301 ataccttgtg gtgaaaccgt cagttatcag caactggcta acgccatcgg caaaccgaaa
    15361 gcggtacggg ccgttgccag cgcctgtgcc gccaacaagc tggctatcgt aataccctgt
    15421 catcgggtgg tccgtggtga tggcacactt tccggttacc gctggggcgt gtcgcgtaaa
    15481 gcgcaactgc tgcgccgcga agctgaaaat gaggagaggt aatgttggat ctgtttgccg
    15541 atgctgaacc gtggcaagag ccactggcgg ctggtgcggt aattttacgg cgttttgctt
    15601 ttaatgctgc ggagcaacta atccgcgata ttaatgacgt tgccagccag tcgccgtttc
    15661 gccagatggt cgctccaggc ggatatacca tgtcggtggc gatgaccaac tgtgggcatc
    15721 tgggctggac gacccatcgg caaggttatc tctattcgcc cattgatccg caaacaaata
    15781 aaccgtggcc cgctatgcca caaagctttc atgatttatg tcaacgtgcg gctacggcgg
    15841 cgggctatcc agatttccag ccagatgctt gtcttatcaa ccgctacgct cctggcgcga
    15901 aactgtcgct gcatcaggat aaagacgaac cggatctgcg cgcgccaatt gtttctgttt
    15961 ctctgggctt acccgcgatt tttcaatttg gtggcctgaa acgtaatgat ccgctcaaac
    16021 gtttgttgtt ggaacatggc gatgtggtgg tatggggcgg tgaatcgcgg ctgttttatc
    16081 acggtattca accgttgaaa gcggggtttc atccactcac caccgactgc cgctacaacc
    16141 tgacattccg tcaggcaggt aaaaaagaat aaaaataaga attattattg ctgtgcgcgc
    16201 gaagatgttt aaactgcggg ctgtaattca ttgtccgggt tttctgcatg gaacttcttg
    16261 tacttgtctg gcggcagtat cgctggccat ttatcagtgt gatggcgcta agcctcgcca
    16321 gtgcggcatt aggcattggc ttaattgctt ttatcaatca gcgccttatc gaaacggcgg
    16381 ataccagtct gctggtgtta ccggagtttc tgggattatt gctgctgttg atggcagtca
    16441 ctctcggatc gcaactggcg ctcaccactt tggggcatca cttcgtttac cgactgcgta
    16501 gtgaatttat caagcggatt ctggataccc acgtcgagcg cattgaacaa ctcggtagcg
    16561 cctcgttgct ggcgggatta accagcgatg tgcgcaatat caccattgct tttgtgcgtc
    16621 tgccggaact ggggcagggg atcattctca ctatcggttc atcggcgtat ctgtggatgc
    16681 tgtcgggcaa aatgttgctg gtaacggcta tctggatggc gatcaccatc tggggcggtt
    16741 ttgtgctggt ggcgcgggtg tacaaacata tggcgaccct acgtgaaacc gaagacaagc
    16801 tgtacacgga ttttcaaact gtacttgaag ggcgcaaaga gctgactctg aaccgggaac
    16861 gcgccgagta tgtgtttaac aacctctaca tccccgatgc gcaagagtat cgccatcata
    16921 ttattcgcgc agacaccttc catcttagtg ccgtgaactg gtcaaacatc atgatgctgg
    16981 gcgctatcgg cctggtgttc tggatggcga acagtctcgg ttgggctgat accaacgttg
    17041 ctgcgaccta ttcgttgacg ctgttattcc tgcgtacgcc gctgctttcg gcggttggcg
    17101 cattgccgac gctgctgacg gcgcaggtgg cgtttaacaa gctgaacaaa ttcgcgctcg
    17161 cgcctttcaa agctgagttt ccgcgcccgc aggcgtttcc caactggcaa acgctggagc
    17221 tgcgtaacgt gacgtttgct tatcaggata acgcgttttc cgttggtccg attaatctca
    17281 ccatcaaacg tggcgagctg ctgtttctga ttggcggtaa cggtagcgga aaatcgacgc
    17341 tggcgatgtt gttgacgggc ttgtatcagc cacaaagcgg cgaaatcttg ctggatggca
    17401 aacctgtcag cggcgaacaa ccggaagatt atcgcaaact gttttcggca gtgtttaccg
    17461 atgtctggct gtttgatcaa ctgctggggc cggaggggca acccgctaac ccgcaactgg
    17521 ttgagaagtg gctggcgcag ctgaaaatgg ctcataagct tgagttaagc aacgggcgta
    17581 ttgttaacct gaagttatca aaagggcaga aaaaacgcgt ggcgctgttg ctggcgctgg
    17641 cagaagaacg cgatattatc ctgctggatg aatgggcggt ggatcaggat ccacacttcc
    17701 gtcgtgagtt ttatcaggtg ttgctgccgc tgatgcagga gatgggtaaa actattttcg
    17761 ctatcagtca tgatgatcat tactttatcc acgccgaccg cctgctggaa atgcgcaatg
    17821 ggcaacttag cgagctgacg ggcgaagagc gcgatgccgc ttcgcgtgat gccgttgccc
    17881 ggacggcata atattacgac gctgacctgg cggactgctg ccgtcaggtc aatatgctaa
    17941 aaatcccttc atttctaata accctataat taatcaacga aattataatg tttctaaaat
    18001 tagaatataa tttataaaca ttatttaaat gttgttactt aagtgttacc gttgatgccg
    18061 cgcaaatctc acctgcaata aagcgactaa aagtaaggca ttaacaagat gaaaaaagtg
    18121 actgccatgc tcttctcgat ggccgtgggg cttaatgccg tttcgatggc ggcaaaagcg
    18181 aaagcgtccg aggagcagga aactgatgta ctgttgattg gcggcggcat tatgagcgcc
    18241 acgttgggga cctattgacg cgagctggag cctgaatggt cgatgaccat ggtggagcgc
    18301 ctggaggggg gcgcgcagga gagttcgaac ggctagaaca acgccggaac cgggcattct
    18361 gcactgatgg aactgaacta caccccgcaa aacgccgatg gcagcatcag tattgaaaaa
    18421 gcagtcgcca ttaacgaagc atttcagatt tcccgccagt tctgggcgca ccaggtcgag
    18481 cgcggcgtgc tgcgtactcc gcgttcattt atcaataccg ttccgcatat gagctttgtc
    18541 tggggcgagg ataacgttaa tttcctgcgc gcccgttacg ccgcgttgca acaaagctcg
    18601 ctgtttcgcg gtatgcgtta ctctgaagat cacgcgcaga tcaaagagtg ggcaccgtta
    18661 gtgatggaag ggcgcgatcc gcaacagaaa ttggcactga tgtgaactat ggtgagatca
    18721 cccgccagtt aattgcttcc ttacagaaga aatctaactt ctcgctgcaa ctcagcagcg
    18781 aagtccgcgc cctaaagcgt aatgacgata acacctggac cgttaccgtt gccgatctga
    18841 aaaacggcac tgcacagaac atccgcgcca aatttgtctt tatcggcgcg ggcggtgcgg
    18901 cgctgaagct gttacaggaa tcggggattc cggaagcgaa agactacgcc ggtttcccgg
    18961 tgggcggaca gttccttgtt tcggaaaatc cagacgtggt taatcgccat ctggcgaaag
    19021 tttacggtaa agcatccgtt ggcgcaccac cgatgtcggt tccgcatatc gatacccgtg
    19081 ttctggacgg taaacgcgta gtgctgtttg ggccatttgc caccttctca accaaattcc
    19141 tcaaaaacgg ttcattgtgg gatctgatga gttccaccac cacctctaac gtgatgccga
    19201 tgatgcacgt cgggctggat aatttcgatc tggtgaaata tctggtgagt caggtgatgt
    19261 tgagtgaaga ggatcgtttt gaagcgttga aagagtacta tccgcaagcg aaaaaagagg
    19321 actggcgttt gtggcaagcg gggcagcgtg tgcagattat caagcgtgat gccgagaaag
    19381 gtggcgtact gcgtctgggt accgaagtgg tcagtgacca gcaagggacc attgcggcgc
    19441 tgctgggggc atcgccaggg gcgtcaaccg ccgcgccgat tatgttgaat ctactggaaa
    19501 aagtatttgg cgatcgcgtt tccagcccgc aatggcaggc tacgttgaaa gcgatcgttc
    19561 cgtcttatgg acgcaagctg aacggtgatg tagcggcaac agaacgcgag ttgcagtaca
    19621 ccagcgaagt gctggggctg aaatacgaca aaccgcaagc agcagatagt acgccgaaac
    19681 cgcagttgaa accgcaaccc gttcaaaaag aagtggcgga tattgcgttg taatgatgtg
    19741 gcgtatactg ataagacgcg ccagcgtcgc atcaggcaac cggctcgggc gttagatgtt
    19801 ggatgcggcg taaccgcctt atccaaccaa cggggtgccg tacttcacaa cagtttagcg
    19861 aacttccgcg ttgtcaattt tctcttccgc cttccagacg cggtatttca catctacatt
    19921 gtctggcgta tacaccacga tcggcagctt gctgttgtaa cgcagcattc cagcatcgcc
    19981 cagatacgcg gtgacaaatt tcttctcttt cttgccatcc gggcaggcca tcatcgtgga
    20041 aaccggcgag ctaactttat caaagacata ataatcatag ccccagcctt ccagcgtttt
    20101 gctttccagc ttcccgccga gacgatgcaa attgcaatcg acttccagcg tctgaccgat
    20161 taacagttct actttcaggg tagattcatc ttcttgcggg gttaactgaa tcacctgacg
    20221 cttcatgcct gtttcagctt gtggataagg cgcgattttt tccagtggct ggacgctttc
    20281 tgccgcccag gcggaagtgg tagcgaaagc ggcaaacaat acttcaggta gaatggtctt
    20341 catttttttg ctttccttct tcatttatag gttttcaccg atgatgtcgc tgaaggttaa
    20401 catattcatc agatttatta atttgcttta tgaataactt aaagaacatt cagacaattt
    20461 tacctgctct tttagtgtta aattctgatg agggagcaaa ccagcagaaa tatctctggt
//
LOCUS       DAZPCS010000060        20448 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZPCS010000060 DAZPCS010000000
VERSION     DAZPCS010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20448
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.060"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(57..1706)
                     /gene="actP"
                     /locus_tag="ACHUQ7_002124"
     CDS             complement(57..1706)
                     /gene="actP"
                     /locus_tag="ACHUQ7_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFR1135530.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTSADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVADLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1703..2017)
                     /locus_tag="ACHUQ7_002125"
     CDS             complement(1703..2017)
                     /locus_tag="ACHUQ7_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HFR1135531.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(2217..4175)
                     /gene="acs"
                     /locus_tag="ACHUQ7_002126"
                     /pseudo
     CDS             complement(2217..4175)
                     /gene="acs"
                     /locus_tag="ACHUQ7_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
     gene            4568..6004
                     /gene="nrfA"
                     /locus_tag="ACHUQ7_002127"
     CDS             4568..6004
                     /gene="nrfA"
                     /locus_tag="ACHUQ7_002127"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFR1135532.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6049..6615
                     /gene="nrfB"
                     /locus_tag="ACHUQ7_002128"
     CDS             6049..6615
                     /gene="nrfB"
                     /locus_tag="ACHUQ7_002128"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFR1135533.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQASPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6612..7283
                     /gene="nrfC"
                     /locus_tag="ACHUQ7_002129"
     CDS             6612..7283
                     /gene="nrfC"
                     /locus_tag="ACHUQ7_002129"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFR1135534.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7280..8236
                     /gene="nrfD"
                     /locus_tag="ACHUQ7_002130"
     CDS             7280..8236
                     /gene="nrfD"
                     /locus_tag="ACHUQ7_002130"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFR1135535.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8256..9974
                     /locus_tag="ACHUQ7_002131"
     CDS             8256..9974
                     /locus_tag="ACHUQ7_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFR1135536.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQENSLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            9967..10350
                     /gene="nrfF"
                     /locus_tag="ACHUQ7_002132"
     CDS             9967..10350
                     /gene="nrfF"
                     /locus_tag="ACHUQ7_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFR1135537.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10347..10943
                     /gene="nrfG"
                     /locus_tag="ACHUQ7_002133"
     CDS             10347..10943
                     /gene="nrfG"
                     /locus_tag="ACHUQ7_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFR1135538.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11285..12598
                     /gene="gltP"
                     /locus_tag="ACHUQ7_002134"
     CDS             11285..12598
                     /gene="gltP"
                     /locus_tag="ACHUQ7_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFR1135539.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12676..13365)
                     /gene="yjcO"
                     /locus_tag="ACHUQ7_002135"
     CDS             complement(12676..13365)
                     /gene="yjcO"
                     /locus_tag="ACHUQ7_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFR1135540.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13459..15606)
                     /gene="fdhF"
                     /locus_tag="ACHUQ7_002136"
                     /pseudo
     CDS             complement(13459..15606)
                     /gene="fdhF"
                     /locus_tag="ACHUQ7_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
     gene            complement(15804..17270)
                     /gene="mdtP"
                     /locus_tag="ACHUQ7_002137"
     CDS             complement(15804..17270)
                     /gene="mdtP"
                     /locus_tag="ACHUQ7_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HFR1135541.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(17267..19309)
                     /gene="mdtO"
                     /locus_tag="ACHUQ7_002138"
     CDS             complement(17267..19309)
                     /gene="mdtO"
                     /locus_tag="ACHUQ7_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFR1135542.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIYGQTFPAMLDYPEVVVRLTLWCIVVG
                     LYPTLLMTLIGVLWFPNRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREALALQ
                     KLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKLASE
                     INKLQHAVAEGQCWQSDWRLSESEAVAARECNLENICQTLLQLGQMNPNTPPTPAAKP
                     PSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVGSSY
                     QKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERSSYI
                     GTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAMIYTFVWPESEARTLPQ
                     KLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLDSEE
                     RALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGLATA
                     LSRSPQITLEETPTSQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGATQ"
     gene            complement(19309..20340)
                     /gene="mdtN"
                     /locus_tag="ACHUQ7_002139"
     CDS             complement(19309..20340)
                     /gene="mdtN"
                     /locus_tag="ACHUQ7_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFR1135543.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMTTVRAPFDGR
                     IISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(20359..>20448)
                     /locus_tag="ACHUQ7_002140"
     CDS             complement(20359..>20448)
                     /locus_tag="ACHUQ7_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1135544.1"
                     /translation="RANINLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         4564 a         5430 c         5622 g         4832 t
ORIGIN      
        1 atgcatgttg gagaggaagc gtattccccg gtcaggcgac cggggaaggg gagagattaa
       61 tgtgcgcggc cttgctcaac gccaaagccg gtctgggagc ggataaactg cgcgcggaac
      121 agttcacgct cgcgcgcccc ttccgctgag ttatcggttg ccgagaagaa ccagatgccg
      181 aggaatgcca cgctgataga gaacagcgcc gggtattcat acgggaagat ggctttttcg
      241 tgaccgagga tctgtaccca aatagtcggg ccgaggatca tcagcaccac tgcggtaatc
      301 agccccagcc agccgcccag catcgcgcca cgggtagtca gtttcgacca gtacatggaa
      361 agcagaatga tcgggaagtt acagctcgcc gcgatggcaa acgcaaggcc taccataaag
      421 gcgatgttct gattctcaaa cagcacgccg aggataatcg caatcacgcc gaggatcagt
      481 acggtgattt ttgatacccg cagctcttca cgttcggtcg cgcctttttt gaagacgtta
      541 gcgtacaagt catgcgaaac cgccgatgcg cccgccagcg tcagatccgc aaccaccgcg
      601 aggatagtgg cgaaagcaac cgctgaaata aaaccgagga ataggttgcc gcccactgca
      661 ttcgccaggt gaacggccgc catgttgtta ccaccaatca gatggcccgc cgcgtcttta
      721 tattccggat tcgcaccaac aagcatgatc gcgccgaagc cgataataaa ggtcagaata
      781 tagaagtagc ccataaatcc ggtggcgtag aacacgctct tacgtgcttc gcgggcatcg
      841 ctgactgtaa agaagcgcat cagaatgtgc ggcaagcccg ccgtaccaaa catcagtccc
      901 agccccagag agagcgcgga gatcggatct ttcaccagtc cgcccggttt catgatgtcg
      961 acacctttcg ggtgtaccgc catcgcttcg ctgaacagat tgttgaagct aaagccgacg
     1021 tgtttcatca ccataaaggc cataaagctg gcaccgaaca gcaacagcac agctttgata
     1081 atttgtaccc aggtggtcgc cagcatgccg ccgaacagga cgtacatcat catcagcacg
     1141 ccgaccagca ccaccgcaat gtgatagtta aggccaaaca gcagctcgat cagtttacct
     1201 gcgcctacca tctgagcgat aaggtaaagc gccaccacta ccagagaacc acaggccgaa
     1261 agaatacgaa tcggcccttg tttcagacga taagaggcca catcggcaga ggtgtagcgt
     1321 cccaggttac gcagacgttc ggcaatcaga aacagaatga ttggccagcc caccaggaag
     1381 cccaacgagt agatcagccc gtcatagccg gaggtaaaca ccagcgcgga gatccccaag
     1441 aatgaggcgg cggacatata gtccccggca atcgccagcc cgttctggaa gccagtgata
     1501 ttgccgcctg cggtgtagta gtcgctacga gaacgtacgc gttttgatgc ccagtaggta
     1561 atgccgagcg taaacacgac gaaaatcagg aacataataa tcgcctgcca gttcgttggc
     1621 tggcgctcta cggccccgct aatagcatcc gcggcgttag ctgcgaaagg gagtgtggcg
     1681 gcaagcgccg tcagaactct tttcatgatg cttgtacctc atgcaggact tcattattaa
     1741 gacggtcgaa ttcgccgttc gcccgccaga tgtagatacc ggtgagaaca aaggagatca
     1801 caatcactcc aacaccaatt ggaataccgc gtgtgacgct ggtgttcgga ttcagcggcg
     1861 tgcccagcca gccgggcgcg aaggcgatca gtaaaataaa gccgatataa actgccagca
     1921 taataatcga caggatggtg gcaaaccgtt gccgtttttc gactaactcc ctgaaatgcg
     1981 cattgtcttc tatccgctga taaatcgtac cattcatcac agattctcca gaggtaatgt
     2041 agggattgtt ttaattcccg ctcccttatg ggagaaggtt aacgctcggg taacccttgc
     2101 cgaatgtagg ccggataagg cgtttacgcc gcatccggca atcaatgcct gatgcgacgc
     2161 tgtcgcgtct tatcaggcct acaaaccgtt accgattcgc atccggcaat tgtgggttac
     2221 gatggcatcg cgatagcctg cttctcttca agcagcttct cgaccacgcc aggatcggca
     2281 agcgtcgagg tatcgcccag gttgctggta tcgcccgccg caattttgcg cagaatacgg
     2341 cgcataattt tgccggagcg ggttttaggc agggagtcgg tccagtgcag cacgtctggc
     2401 gtcgccagcg ggccaatctc tttacgcacc cagttgcgga cttctgcgta cagttctggt
     2461 gacggttcct ccccgtgatt aagcgtgaca taggcgtaga tcgcctgacc tttaatattg
     2521 tgcggaatac ctactacggc ggcttcggca atcttcggat gcgccaccag cgccgactct
     2581 atctctgccg tccccagacg gtgaccggag acgttcagca cgtcgtccac acgcccggtg
     2641 atccagtaat agccatcttc atcgcgacgc gcgccgtcgc cgctgaaata catatttttg
     2701 aaggtggaaa aataggtctg ctcaaaacgt tcgtgatcgc caaacagcgt acgcgcctga
     2761 ccaggccagg agtcggtgat caccaggcta ccttcggtag ccccttccag cgggttacct
     2821 tcgttatcga ccagcgccgg ttgcacgccg aagaacggac gtgttgccga accggctttc
     2881 agctcggtag cgccaggcag cggggtgatc atgaaaccgc cggtttcggt ctgccaccag
     2941 gtatcgacca ccggacattt ctcgttgccg atttttttcc agtaccactc ccacgcttcc
     3001 gggttaattg gctcgcccac ggaaccgaga atgcgtagcg acgaacggtc ggtgccttca
     3061 atcgctttat cgccttccgc catcagcgcg cggatcgccg tgggtgcggt atagagaata
     3121 ttgacctgat gcttgtccac cacctgcgcc atacgggcag gcgtcggcca gttcggtacg
     3181 ccttcaaaca tcagcgtggt tgcgccgcag gccagcgggc cgtacagcaa ataactgtgt
     3241 ccggtaaccc agcccacatc ggcggtgcac cagtagatat cacccggatg ataatcaaag
     3301 acatatttaa aggtcagcgc cgcgtacacc agataaccgc cggtagtgtg cagtacgcct
     3361 tttggttttc cggtagaacc ggaggtatag agaataaaca gcggatcttc ggcgttcatc
     3421 tcttccgcct agtgctgatc gctggcttgc tcaaccaggt cgtgccacca caggtcgcgc
     3481 ccttcctgcc agtcgatgtt tccgccagta cgcttcagta ccaccacatg ctctacgctg
     3541 gtgacgttcg ggttttttag tgcgtcatca acgtttttct tcagcggaat actacgcccg
     3601 gcgcgcacgc cttcgtcgga agtgatcacc agtcgtgagt tggaatcaat aatgcgcccg
     3661 gcaacggctt ccggcgagaa gccgccgaaa atcaccgaat gcatcgcgcc aatgcgggcg
     3721 caggccagca tcgcaaccgc ggcttccggc accatcggca tataaatcgc caccacatca
     3781 ccttttttaa tgcccagctc gagcagggta ttggcgaagc ggcagacgtc gcggtgcagc
     3841 tctttatagc tgatatgttt gctctggctg gcgtcgtcgc cttcccagat gatggcggtg
     3901 cgatcgccgt tttcttgcag atggcggtca aggcagtttg ccgccagatt cagcgtgccg
     3961 tcctcgtacc atttaatgga cacattaccg ggggcaaagg aggtattttt caccttctgg
     4021 tacggtttga tccagtcgag aatttttccc tgttcgcccc agaaggtatc aggtacgtta
     4081 atagattgtt gatacatcgc ctcgtactgc tgagggttta tcaggcaacg gtctgcgatg
     4141 ttggcaggaa tggtgtgttt gtgaatttgg ctcatgcttt tgttctcctt gtaggatgtt
     4201 aataatatgt ggcataagcg ttaaatgtag gggtattggc agttttgttt agtatttggg
     4261 cgacagatca cgcaaaagta gaatggtgca aataaacggc agggtaattt ttgaaggtca
     4321 ccaacaaaag ttgattaatt ctttgaggaa catgcagtta tgcatgctgt tgaaaaagag
     4381 gaagatactg actaactcta aagtggtatt tcacatgcac ttacaattga ttaaagacaa
     4441 cattcacagt gtggttattt gttacacata ggggtgagca atgtcatgac agtgtaggtg
     4501 cggttactgt cgtgaaaaac aataaaaacc gccattgcaa caatggcgca attcggatga
     4561 agcccctatg acaaggataa aaataaacgc acgccgtatc ttcagcttat tgattccttt
     4621 tttctttttc acttctgttc acgctgaaca aacggcagct cccgcaaaac ctgtaactgt
     4681 ggaagcgaag aatgaaacct ttgccccgca gcatcacgat caatatctct cctggaaagc
     4741 cacctcggaa cagtcagagc gtgttgacgc cctggcggaa gatccacggc tggtgatcct
     4801 gtgggcgggg tatcccttct cgcgcgatta caacaagcct cgtggacatg cctttgctgt
     4861 gaccgatgtg cgtgaaaccc tgcgtaccgg tgcgccgaaa aacgctgaag atggtccgct
     4921 accgatggcg tgctggagtt gtaaaagccc ggatgtggcg cgtctgatcc agaaagacgg
     4981 cgaagatggc tacttccacg gtaaatgggc gcgcggcggc ccggaaatcg tcaacaactt
     5041 aggttgtgcc gactgccata acaccgcctc accagagttc gccaaaggca aaccggagtt
     5101 aaccctttcc cgtccgtatg cggctcgcgc gatggaagcc attggtaaac cttttgagaa
     5161 agccggacgt ttcgaccagc aatcgatggt ttgcggtcag tgccatgtgg agtattactt
     5221 cgacggcaaa aacaaagcgg ttaaattccc gtgggatgac ggcatgaaag tcgaaaatat
     5281 ggagcagtat tacgacaaaa ttgccttctc tgactggact aactccctgt cgaaaacgcc
     5341 aatgctgaaa gcgcagcacc cggaatatga aacctggaca gcgggcattc acggtaaaaa
     5401 caacgtgacc tgtatcgact gccatatgcc aaaagtgcag aacgccgaag gcaaactcta
     5461 caccgaccat aaaattggta atccgtttga taacttcgcc cagacttgtg cgaactgcca
     5521 tacccaggat aaagctgcct tgcaaaaagt ggtcgcggaa cgtaagcaat cgattaacga
     5581 cctgaaaatc aaggttgaag atcaactggt tcacgctcac ttcgaagcga aagcggcgct
     5641 ggatgcaggc gcgacggaag ccgaaatgaa gccgattcag gacgatatcc gtcatgccca
     5701 gtggcgttgg gatctggcga ttgcttccca cggtattcat atgcacgcac cggaagaagg
     5761 tctacggatg ctcggtacgg cgatggataa agcggcggat gcgcgcacca aactggcgcg
     5821 cctgctggcg accaaaggta tcacccatga aatccagatc ccggatatct cgaccaaaga
     5881 gaaagcccag caggccattg gcctgaacat ggaacaaatc aaggccgaga agcaggactt
     5941 catcaaaacg gtgatcccgc agtgggaaga gcaggcacgt aaaaacggtc tgttaagcca
     6001 ataaccccgt tccgcctcgc aaggggcgga aaacacaatg gagtgaatat gagcgtatta
     6061 cgttcgttat taactgctgg ggtgctggcg tcgggcctgt tgtggagcct gaacggaatt
     6121 accgctaccc ctgcggtgca ggcatctggt gatcgctacg aagttaccca gcagcgtaac
     6181 ccggatgccg cctgtctgga ctgtcataaa ccagataccg aaggtatgca tggcaaacat
     6241 gcttccgtta tcaacccgaa taacaaattg ccggtcacct gcaccaactg tcacggccaa
     6301 gcatcaccgc aacaccgcga aggggtgaaa gatgtgatgc gctttaacga gccgatgtat
     6361 aaggtcggag agcagaacag cgtctgtatg tcctgtcatc tgccggaaca gttgcaaaaa
     6421 gcgttctggc cgcacgatgt ccacgtaacc aaagtggcgt gcgccagctg ccattccctg
     6481 catccacagc aagatacgat gcagacgtta agtgacaaag gacggattaa gatttgcgtc
     6541 gattgccaca gcgatcagcg caccaatccg aactttaacc cggcgtctgt tccgttgctt
     6601 aaggagcagc catgacctgg tctcgtcgcc agtttctcac cggcgtcggc gtgctggcag
     6661 ccgtcagcgg caccgcaggg cgcgtggtgg cgaaaacgtt gaatatcaat ggggtgcgtt
     6721 acggcatggt gcatgacgag tcgttatgca tcggctgtac cgcctgtatg gatgcttgtc
     6781 gggaagtgaa caaggtgccg gaaggcgtct cgcgcctgac gattattcgt agcgagccgc
     6841 agggcgaatt tcctgatgtg aaatatcgct tcttccgtaa gtcttgccag cactgcgatc
     6901 atgcgccgtg cgttgacgtc tgcccgaccg gtgcgtcttt tcgtgatgcc gctagcggga
     6961 ttgtcgatgt taatcctgat ctctgcgtcg gttgtcagta ctgcatcgcc gcctgtccgt
     7021 accgcgtgcg ctttatccat ccggtcacga agacggcgga caaatgcgat ttctgtcgta
     7081 agaccaattt gcaggccggt aagcttcccg cgtgcgttga agcttgcccg acgaaggcgc
     7141 tgacgtttgg caatctggac gatcctaaca gtgagatttc gcaactgctg cgccagaagc
     7201 ctacttaccg ctacaagctg gcgctgggaa ccaaaccgaa gctgtaccgc gtaccgttta
     7261 aatacgggga ggtgagccaa tgacgcagac ttccgcattt cattttgaat cgctggtgtg
     7321 ggactggccg attgccatct acctgttttt gattggtatt tctgccggtc tggtgacgct
     7381 ggccgtgctg ttacgtcgct tctacccgca ggcgggcggt gcagacagta cgttgctgcg
     7441 caccacgctg attgtcgggc cgggcgcggt gatcctcggt ctgttgatcc tcgtcttcca
     7501 cctgacaaga ccgtggacct tctggaagct gatgttccac tacagtttta cctcggtgat
     7561 gtcgatgggg gtgatgctgt ttcagctcta catggtggtg ctggtgctgt ggctggcgaa
     7621 aatctttgaa catgatttgc ttgccctgca acaacgctgg ttgccgaagc tggggatcgt
     7681 gcaaaaggtt ctgagcctgc tgacgcccgt tcatcgcgga ctggaaacgc tgatgctggt
     7741 gttggcggtg ctgctggggg cttataccgg ctttctgctg tcggcgctga aatcgtatcc
     7801 gttcctcaat aacccgagcc tgccggtgct gctcctcttc tccggcatct cgtccggtgc
     7861 ggcggtggcg ctgatcgcca tggcgatacg ccaacgcagt aacccgcatt ccacggaagc
     7921 gcactttgtt caccggatgg aaatccccgt ggtatggggt gaaatcttcc tgctggtggc
     7981 gttttttgtc ggtctggcgc tgggcgatga cggtaaagtt cgtgcgctgg tggcggcatt
     8041 aggtggcggt ttctggacgt ggtggttctg gcttggtgtc gccgggctgg ggctgattgt
     8101 gccaatgttg ctcaaaccgt gggtcaatcg cagttccggc attcctgccg tgctggcggc
     8161 gtgtggggcc agtctggtcg gcgtgttgat gctgcgcttt ttcattctct acgccggaca
     8221 gttgacggtg gcgtaagcca gaaaagaggt ggtttctgga cgtattcctt cctgaagtcg
     8281 gttttctggc gttgttgtta agtctcgggg tcaacgtgtt gaccccgttg acggcctttg
     8341 cgggagtgcg gttgcgctgg cctgccatga tgcgactcac ttgcatcggc attctggcgc
     8401 agttcgcgct cctgctgctc gcctttggcg tactgacgta ttgttttctc atcagcgatt
     8461 tctcggtcat ttatgtcgcc caacatagct acagcctgct gtcgtgggaa ctcaaactgg
     8521 cggcggtgtg gggcggtcat gaaggttcgc tgctgctttg ggtgctgctg ctttccgcct
     8581 ggagcgcgct gtttgcctgg cattatcggc agcaaagcga tccgctattt ccgctgacgc
     8641 tagccgtttt atctctcatg ctcgccgcac tgctactgtt tgtggtgctg tggtccgatc
     8701 ccttcgtgcg gatatttcca ccagcaatcg aaggccgcga tctcaatccg atgctgcaac
     8761 atcccggtct tatctttcat ccaccgctgc tttaccttgg ctatggcggt ttgatggtag
     8821 cggcgagcgt ggcgctggcg agtctactgc gcggcgagtt tgatgcggcc agcgcccgaa
     8881 tttgctggcg ctgggcgtta cctggctgga gtgcattaac ggcggggatc atcctcggtt
     8941 cctggtgggc ctactgcgaa ctcggctggg gcggctggtg gttctgggat ccggtggaaa
     9001 acgcctcttt attaccctgg ctttctgcca ctgcgctgct gcacagtttg tccctgacac
     9061 gccagcgggg gattttccgc cactggtcgc tgttgctggc gatagttact ctgatgctgt
     9121 cgctgctggg caccttaatt gtccgttctg gcattctggt ttcggttcat gcgttcgcac
     9181 tggataacgt ccgcgccgtg ccgttgttca gcctgtttgc actgattagc cttgcgtctt
     9241 tggctctgta tggctggcga gcgcgggacg gtggcccggc ggtgcgtttt tcggggttat
     9301 cgcgggaaat gttaatcctc gctacgctgt tgctgttttg cgcagtgcta ctgatcgtgc
     9361 tggtgggaac gctttatccg atgatttacg gcctgctggg ctggggacgc ctctccgttg
     9421 gcgcgccgta ttttaaccgc gcgacgttac cgtttggtct gttgatgctg gtggtgattg
     9481 tgctggcgac gtttgtctct ggcaaacgcg tgcagcttcc ggcgctggta gctcatgctg
     9541 gcgtgctgtt atttgccgcg gggatcgtgg tttccagtgt cagtcgtcag gagaacagcc
     9601 tgaatttaca gccgggtcag caggtgacgc tggcaggata caccttccgt tttgagcgac
     9661 tcgatctgca agccagaggc aattacacca gcgaaaaagc gatagtggcg ctgtttgacc
     9721 atcagcaacg cattggtgaa ttaacgccgg agcggcgttt ttatgaagca cgccgtcagc
     9781 aaatgatgga accgtcaatt cgctggaacg gcatccatga ctggtatgcg gtcatgggtg
     9841 aaaaaaccag agcggatcgt tacgcttttc gtttgtatgt acaaagcggt gtgcgctgga
     9901 tctggggggg aggattgttg atgattgcgg gcgcattgtt aagcggatgg cgggggaaga
     9961 agcgcgatga ataaagggct tctcacgcta ctgctgttat ttacctgttt tgcgcgcgct
    10021 caggttgtag acacctggca attcgccaat ccgcaacaac agcaacaggc gttaaatatt
    10081 gccagccagt tacgttgtcc gcagtgccag aatcaaaact tactcgaatc caatgcgccg
    10141 gtggcggtca gtatgcgcca tcaggtttat agcatggtgt cggaggggaa gaacgaagtc
    10201 gaaatcattg gctggatgac cgaacgctac ggagattttg ttcgctataa cccaccgtta
    10261 acgggtcaga cgctggtgtt gtgggcgctg ccagtggtgt tgttattgct gatggcactg
    10321 atcctctggc gagtgagggc taagcgatga aacagcctca aataccggtg aaaatgctga
    10381 caacactcac gatattgatg gtattcctct gtgtcggcag ttatctgtta agcccaaaat
    10441 ggcaggcggt acgtgcggag tatcagcgtc agcgcgatcc gctacatcag tttgccagcc
    10501 agcaaacccc ggaagcgcag cttcaggcat tgcaggataa aatccgcgct aatccacaaa
    10561 acagcgaaca atgggcgtta ctgggcgagt actatctgtg gcaaaacgat tacagcaatt
    10621 cgctgctggc gtatcgtcag gcgttgcaac tacgtggtga gaacgctgaa ctgtatgcgg
    10681 cgctggcgac ggtgctttat taccaggcca gccagcatat gaccgcccag actcgcgcaa
    10741 tgatcgacaa agccctcgcg ctggactcta acgaaatcac cgctctgatg ctgctggctt
    10801 ccgatgcgtt tatgcaggcg aattacgcgc aagccatcga actatggcaa aaagtgatgg
    10861 atctcaactc accccggatc aaccggacac agctggtcga gtcgattaat atggcaaaac
    10921 tgttgcagcg gagatcggat taatcatctg gtgatgaaga ttatgtcatt gagctgcata
    10981 aaaaataatc gaatgaacat atgccaaaaa taatcaccaa tcagtattat tgcagattaa
    11041 acaaataaaa atctttccat aacaaatggt tattcattaa tcctgccata aactcgcgtt
    11101 attatgcatt aatgcagcga aaagctctgt tgttaaaggg ttgcgcaaca taccgcgcaa
    11161 atgatactga tcataagcgt taaaaaaatc tacaaaccaa cgcaacacaa ttcatgccct
    11221 ggcagtatgt cacgttctcg cgtttctgaa cggggaacgg cgctccattg aggaagttat
    11281 tctgatgaaa aatataaaat tcagcctggc ctggcagatt ctgtttgcta tggtgctggg
    11341 cattctcctg ggaagctacc tgcactacca tagcgacagc cgcgactggc tggtcgtcaa
    11401 tttgctctct ccggcgggtg atatcttcat ccatctgatc aaaatgattg ttgtgccgat
    11461 tgtgatctcc acgctggtgg tgggtatcgc gggtgttggt gatgccaaac agctcgggcg
    11521 tattggcgcg aaaaccatta tttacttcga ggtgatcacc accgtcgcca tcattttggg
    11581 gatcactctg gcgaacgtct tccagcccgg tgccggggtg gatatgtcgc agctggcgac
    11641 cgtcgatatc tcgaaatatc agagcactac ggaagcggta caaagcagtt cccacggcat
    11701 tatgggcacg attttgtcgc tggtgccgac gaacattgtg gcgtcgatgg cgaaaggcga
    11761 aatgctgccg atcatctttt tctcggtgct gtttggtctg gggctttctt ccctgcccgc
    11821 gacgcatcgt gaaccgctgg tgaccgtgtt ccgctccatc tctgaaacca tgtttaaagt
    11881 gactcacatg gtgatgcgtt atgcaccggt gggtgtgttt gcgctgattg cggtgacggt
    11941 ggctaacttt ggtttctcgt ctctgtggcc actggcgaaa ctggtgctgc tggtgcattt
    12001 cgccattctg ttcttcgcgc tggtagtgct gggaattgtg gcacgtctgt gcgggttaag
    12061 cgtctggatc ctgattcgta ttctgaaaga tgagctgatt ctggcgtact ccactgccag
    12121 ctctgaaagc gtactgccgc gaattattga gaagatggaa gcctacggag caccggcgtc
    12181 gatcaccagc ttcgtggtgc cgaccggtta ctcttttaac cttgatggtt cgacgctgta
    12241 tcaaagtatt gccgctattt tcatcgctca gctgtatggc attgacctgt ccatctggca
    12301 ggaaatcatt ctggtgctga cgctgatggt gacctcgaaa gggattgctg gcgtggctgg
    12361 cgtgtcgttt gtggtgttgc tggcaacgct gggtagcgta ggtattccgc tggaaggtct
    12421 ggcgtttatt gctggtgttg accgtatcct cgacatggcg cgtactgcgc tgaacgtggt
    12481 gggtaatgcg ctggcggtgc tggtgattgc caagtgggaa cacaaatttg accgcaaaaa
    12541 ggcgctggct tatgagcgtg aagtgctggg caaatttgat aaaactgcgg atcaataatt
    12601 gaagattgcc ggggatatcc cccggcaatg tgtggatgcc tgatgcgacg cttgccgcgt
    12661 cttatcaggc ctaccttaac cgttggttaa tttttcaaac tcttcacacc cggtatcaaa
    12721 cccttccata cagctcaggt tcaaccagtg cagcgccttt tgtttgttct tctcgataaa
    12781 accttcctca ccgtttaaga acatcattcc cgcccagtac tcggaataac cggttcggga
    12841 aattgcagag ctgcgtttga aataccaggt tgccttgtca tcgtcggcct taatgcccac
    12901 gccgttggcg taaatcaaac caagcagcat ttgggcatcg acggcggagt cattctccag
    12961 atcttccgag gcgttttcta acagcgaaat cgcctttgga taatccggtt tacccgcttg
    13021 agtatttacc agaatatgcg ccagcgttac ttcaccttct ttactgcccg cttgcgccgc
    13081 tttctctgca agaacttttg cttgtggata gtccagactg accggattgg tgattttgat
    13141 ctgcgccaac agcgcgcagg catcagcatc accgctgtcg gcggctttct gtgcccaata
    13201 ttcggctttg ctcaaatcgc cggagctaaa ccagctgtcg gcaagaaaat attgtgcacg
    13261 tcggtccccg gcctctgctg cctttaaata ctggctgcca ggctcggagt cgttcgcgtg
    13321 ggcaaagaat gtcaaaaaca acatcaatgc gataattttt ttcattttga taatacgatt
    13381 gggtagggag taaccagtat aaagagttcg aatggataaa aaaacagcct ccgaaaggag
    13441 gctgtagaaa ggactgtatt acgccagtgc cgcttcgcgc aggcgagttt tcaacttgtt
    13501 gtactcgtca atcacgtact gctcggcggc gcgctgatcg gagatcggct cgacgcgcac
    13561 ggcgcagtat ttgtattccg gcgttttcgt aatcgggctt aagctttcgg taaccagctc
    13621 gttacaggca ccaatccacc actggtaggt catgtaaatc gcccctttgt tcggacgatc
    13681 gctgacctgc gcacgggtga taattttgcc tttacgcgag tgcactcaaa ccaatgcctc
    13741 atcttcaata cccagacgtt tggcgtcttc ggtattgatt tgtgcgtagc caggttcatc
    13801 agccagcgcc gccagtgccg cacagttacc ggtcatcgaa cggcaagagt agtgaccaac
    13861 ttcacgcacc gttgacagta ccatcgggta ctcgtcggtg agtttgtcga ttggcgctac
    13921 ccagtcgcag gtgaagaact gcgccagacc gttcggggta tcaaacttct ctttaaacag
    13981 ataagaagtc ccctgatcgg catctgaagt atcgcggcaa ggccactgaa tgaagcccag
    14041 ttcgcccatt ttctcgtaag tcgcaccgta gaaatccggg cacagatgac gcaactcatc
    14101 ccagatctcc tgggtgttgt tgtagtgcat cggataaccc atacgggtgg cgatttcact
    14161 gatgatttgc cagtccgttt tcagatccca tttcggttca actgctttga agaaacgctg
    14221 gaagccacgg tcagccgcag taaacacgcc ttcatgctcg ccccacgacg ttgacggcaa
    14281 aatgacatcc gccgccgacg cggttttggt tataaagatg tcctgaacga tgaccagttc
    14341 cagatcttca aaggctttac gtactgccga cagctccgca tcagtttgca gcggatcttc
    14401 gcccataatg tacgcggcac gcacttcgcc atgcgctgcg cggtgcggca gctcgctgat
    14461 gcgatagccg gtatgagcag gcaggctttc cacgccccag gctttggcga atttttcgcg
    14521 gttagccgga tctttcacgt actggtatcc cggatacgta tccggcagcg cgcccatatc
    14581 gcaggcaccc tgcacgttgt tctgaccacg aaccgggttt acacccgcat gcggcttacc
    14641 gaggttactg gtcagcatcg cgaggctggt cagagaacgc acggtttcca cgccctggta
    14701 gaactgggtt acacccatgc cccacaggat ggcggcgctt ttcgcctggg catacatccg
    14761 tgccgcctga cgaatctcac tggcgctgac accggtgata tcttcaaccg actccggcgt
    14821 gtagccttca acgattttac gatactcttc aaagccttct gtacgggaag cgacgaacgc
    14881 tttgtcgtac agattttctt caataatgac atggcccatc gcattcaaca gcgcgatgtt
    14941 cgagccgttt ttcagtgcaa tgtgcatgtc agcaatgcgc gcggtttcaa ttttgcgcgg
    15001 atcgcagacg ataattttcg ccccgttacg tttagcgtta attacgtgat tcgccacgat
    15061 tgggtgggaa tccgccgggt tgtacccgaa aacgaacact aaatcggtat tatcaatttc
    15121 gttaatagca ttgctcattg cgccattacc gaccgattgg tgcagacctg caaccgatgg
    15181 gccgtgtcag acacgagcgc agcagtcaac gttattggta ccaataacgg cgcgcgcaaa
    15241 tttttgcatt acatagttgg tttcgttacc cgtaccacgc gaggagccgg tcgtctggat
    15301 ggcatccgga ccgtacttct ctttgatggc gctcaggcgc tcggcaacgt aattcagtgc
    15361 ctcatctcag gaaacaggtt cgagtttgcc gccacgctgg cgacggatca tgggggtttt
    15421 caggcgcggg gtcaggatct gggtatcgtt aatgaaatcc cagccataat aacccttcag
    15481 acacagggta ccctggttgg ttttcccctg cgctgcctcc gcccggacga ttttgccgtt
    15541 atcgacgacc aggttgattt tgcaacctga tgcgcaatag gggcaaaccg tgacgacttt
    15601 tttcatcggt ctcgctccag ttaatcaaat cacgcatacg cgctctcgac tacagtatgc
    15661 atcttttatg ccacatttta tttggggtca ttccctgata ttacgggcac tatttattca
    15721 aaactctgac gaaaaacagg ctgtcgtcag ttttgacgtg acgaaacgaa ataccgcgtg
    15781 acagccatca cgcggcagac attttatttt ttctcgacga cgggagccgc ctgatacccg
    15841 ccgcccagcg atttcatcaa ctgaatgctt tggatcaccc ggcggctgtc cagcatcagt
    15901 aatgacatct cttcggcaag tactggcaac cgggcttcag tggcctgtaa gcggctggtt
    15961 aagccgcgct gataggcggc ctcggcagcg cgctgggtga agcgcgttgc ttccacgtgt
    16021 tcagcctgca tttctcgctc gtcgttgagc gtttgcagac gcgtgccgtt gacggcaacg
    16081 tcacgcaccg cgttcagtac tgactggttg taacgttcaa tcatcatgtt gctggcggcg
    16141 cgcgtgcctt cgagattggc attcaaccgt ccaccgtcaa acagcggcaa tttcagaccc
    16201 gggatgaagt tgaactggcg actggttttt ttgaataagg tatccaggtg gatggcatca
    16261 aggccgaaaa acgctttgat atcaaagctc ggatagaaca acgcccgcgc ggaatccacc
    16321 tgatctaatg acgcctgaac ataccagcgc atggcttgca gatccgggcg tctggcgagc
    16381 aactcataag agagtgtcgc cggaatgccg gtctggactc gtggtaatgc cacaggtttg
    16441 atctccggca tatcgctggc tcccgcgcca atcaatgcgc gcagagattc tctcgtttcg
    16501 gtgatttgcc ctttgacggc agcaatttgt ttatcgaccg ccagaatctg tgcccgcgcg
    16561 ccgtggaaag gcacttgtgc ttccagaccg tgcgccactt tactttggtg cgctttcacc
    16621 gcgtaatcaa tcacatcgcg agtttgttct aacagatcga gcatctgata gctggcctgc
    16681 atactgtaat aaagctgcgc tacgcccgtg gtcagcgata gctctactgc tgcggtttct
    16741 gccagcgcgg cattatgcgc gccaatggcg gcggcaaccg ctgagcgatg cacaccccac
    16801 aaatcaagat ccagtccggc aaacaaacct actgtggctt ccgtatagta cggcccgtcc
    16861 atccccagtg cgggcgcatc catcgcataa gggcttaaaa agccgttcgc cgagacacgt
    16921 tggcggttaa gcatccctaa cgctgccacc tgcaactgtg aaccggcatc taacaaatcg
    16981 gcctgcgatt gcgctttttc ttcccgcagt ttcgcttcgg cgagggtgtg tgaaccactt
    17041 agcgtccgtt ggatcagggc atccaactgc gggtcattga gttgtttcca ccactgcgcc
    17101 tgcggccagc cggagctggc aagatgaata tcatcggcca gtttgatttg ttccggtttg
    17161 agctgttgat gaggcgcaga atccttacgt accagggcac agccggaaat cagcgtcgtg
    17221 ctgccgagaa tgctgcacaa cagcagacgt gaaagttgac gattgatcat tgcgtggctc
    17281 cttgtgcctg ttccgtggcg ggcgttaatg ccggggctgt ccagtctggc aagcgggcga
    17341 aaagctggca gacgtgttgc tcctgtttta ataaggtggg caggatggcc tgcgaggtgg
    17401 gtgtctcttc aagcgttatt tgaggagaac ggctgagtgc ggttgccaga ccggcagcgt
    17461 atttctccag ggcgtcggca aactgacctg ctctgtccgg ctgtagtgcg ttatccagcg
    17521 cctgcgccga attccaggtg gcatcccagg cgtgaaggat atcgcggccc tgacgaataa
    17581 ccgtttgcga acgttcaatc agtaaggcgc gttcttcgct gtccagttga cgctccagcg
    17641 ccacgcgttg gcacatctct tcacaggcat taaacgccgc atgcagaccg atacgaattt
    17701 gcagataagt gcgcagagcc gtgacttcct gctggcgtgg gatccgcatt actttactta
    17761 acatacccag cgcgccagcc agtttttgcg gcagcgtgcg cgcttcactt tcaggccaga
    17821 caaaggtgta aatcatcgcg gacaccacgg taccaatgat gatacccagg gcgcgatcgc
    17881 gaatttccac caggtcgtac actgggccaa aaacgttttc gagcgtggcg agcgcgaagg
    17941 tgaccaccat ctgtgtgccg atataagaag agcgttcaga gctggtggca atccatgcgc
    18001 ccaacaggaa aatcggtgcc agcacaaaca gcaattcgac aatattgtcc agccagggca
    18061 tgaccagtag cgtgaataac agcgccagaa tcgcgccgca aaaggccccg ccaaaacgca
    18121 gcaccatctt ctggtacgac gaaccgacat ttggattagc gacgatcacg caggtcagca
    18181 tacaggtgtg aatgccttcc cagtccacgc cgctgtaaaa ggtgtaacag atcaaacagg
    18241 cgagcaatgt ttttaccgcg tagcgtatat agtctggatt ggtaaaagca tcggcgacca
    18301 ttgatggcgg tttggcggcg ggcgttggcg gcgtattcgg gttcatctgg cccagttgta
    18361 acaacgtctg gcagatattc tccaggttac attcccgtgc cgccaccgct tcactttcac
    18421 tgagccgcca gtcgctttgc cagcactgac cttcagcaac ggcatgctgc agcttgttga
    18481 tttctgaggc taatttttgc cgaaattcaa taattgcctg agaatcagca aaagaggtgg
    18541 gatcgtagcg attcagcgtc gagtaaatgt aggttaccgt tgccacgcag ctttgccacc
    18601 atgcgctttg agttcgccag ttggcatcgt ccgcgagaca aaagacattc agtttttgca
    18661 gcgccagcgc ctctctttca atccgcgttt cgggtagcgg tgcgaggctg tccgtcaggt
    18721 ggctaatggc atcatcaagc cgatcattaa gcgcttgatg catttgcgta atggcacgat
    18781 tgggaaacca cagcacgccg attaacgtca tcagcaaggt tggatagagg ccaacaacga
    18841 tacaccacag cgttaagcgc acgactactt ccggatagtc gagcatggcg gggaaggttt
    18901 gcccgtaaat ggcgacagcg aaaaagacca gtcccagccg atgggtgcgc atcaaaaaca
    18961 tgcagcccat caggatcggc ccggcgatga tcaatcggat caacggttcg ccgtatgacc
    19021 atttgtagat caaaaacagg ctgccgatct ccagcaccgt ggcaaccaca aacaagatcg
    19081 ccacaaattt ggtgtaaaac gcgttcgact gaataccgta aaacagcact gccagcgata
    19141 acgccacaaa agggatctca aaagtcatcg agatcagaat caccagcagg cagcctaccc
    19201 agagttgcac gatctgcggc acgcggccgg gtcgccgctc gcttaactct tcatgaaaga
    19261 acgccagcag cctgaccacc ggtaatggca gggagttgag cgcgctcatt attgcggctc
    19321 aagattagcg actgccgaag cgccgatgcg gaacatttcc gggtcaggtt tatcgaccat
    19381 gattttgacc ggaaaacgct gggcaacgcg gacccagtta atagaacgag acacttttgg
    19441 caggccgccc agcaccaggc cgccgtcatc cggtagcacg ccgtagccaa tcgaatccac
    19501 tttaccctcg aaggttttgc cgctgtcact catcaggcga atcgttgcgg gtgtacctga
    19561 gcgaatattt ttcagatcgg tttcgcggaa gttggcgatc acgtaccagt gacgagtgtc
    19621 gatgagggta aaaatagggc gcatggcaga agcaaattgc ccgacggagg ttttgaggga
    19681 aatgatccgg ccatcaaatg gcgcgcgaac ggtcgtcatt tccagatgta gtttggtcag
    19741 ggcaatatcc gcttcgaccg ccgcacgttg cgccaccagc gcatcgacgc cgctgacggc
    19801 gctggcggct gactgcgcct gtaacaatac ggcattaaga tccgcttctg cggcgcgctg
    19861 cgccgttctt gcacggtcaa cgtcttctgc tgagacaaaa ccctctttca gtaatggctc
    19921 ggtgcggcgt aatgtatctg tggcctgttt cgcggcggca cgggcttttt ctaccgtggc
    19981 attaaccgag tcggcaccaa actgttgggc gtcaacgcta cgctgggtga gcataatttg
    20041 cttatccagc gccgcgaggg aggcttcggc tttcgccagg ttggcttcgt acgggcgcgg
    20101 gtcgatgcgg aacagcaaat cgccctgttt caccgcctgg ttgtcggtga ccgccagttc
    20161 tacaatgcga ccgctgactt ccggcaccac atcaatggta tctgctgacg cgtaagcgtc
    20221 attagttgat ggcgcactgt ctacgcgcca gataacgaaa acaagggcaa ccagcgccaa
    20281 cgcaaccact aacagagcag ggaatttact gcgaggtgct tttttcggcg tactttccat
    20341 gagcgcgtct caaatagatt agaaaaatgc cagccagaac agcatggcgt agagagcaaa
    20401 aagggcggta taaataattc cgacaaatgc cagattgata tttgcccg
//
LOCUS       DAZPCS010000061        20151 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZPCS010000061 DAZPCS010000000
VERSION     DAZPCS010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20151
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.061"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            89..865
                     /gene="exuR"
                     /locus_tag="ACHUQ7_002141"
     CDS             89..865
                     /gene="exuR"
                     /locus_tag="ACHUQ7_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFR1135545.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1105..1188
                     /gene="yqiM"
                     /locus_tag="ACHUQ7_002142"
     CDS             1105..1188
                     /gene="yqiM"
                     /locus_tag="ACHUQ7_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HFR1135546.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            1210..1872
                     /gene="yqjA"
                     /locus_tag="ACHUQ7_002143"
     CDS             1210..1872
                     /gene="yqjA"
                     /locus_tag="ACHUQ7_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFR1135547.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1876..2259
                     /gene="mzrA"
                     /locus_tag="ACHUQ7_002144"
     CDS             1876..2259
                     /gene="mzrA"
                     /locus_tag="ACHUQ7_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFR1135548.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2406..2760
                     /gene="yqjC"
                     /locus_tag="ACHUQ7_002145"
                     /pseudo
     CDS             2406..2760
                     /gene="yqjC"
                     /locus_tag="ACHUQ7_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
     gene            2798..3103
                     /locus_tag="ACHUQ7_002146"
     CDS             2798..3103
                     /locus_tag="ACHUQ7_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFR1135549.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3106..3510
                     /locus_tag="ACHUQ7_002147"
     CDS             3106..3510
                     /locus_tag="ACHUQ7_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFR1135550.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3500..3799
                     /locus_tag="ACHUQ7_002148"
     CDS             3500..3799
                     /locus_tag="ACHUQ7_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFR1135551.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDISASSREWLEATGAYDRRWN
                     MLLSLRSWALVGSRVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3985..4281
                     /locus_tag="ACHUQ7_002149"
     CDS             3985..4281
                     /locus_tag="ACHUQ7_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFR1135552.1"
                     /translation="MKKLEDVGVLVAHILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLLTAFLFHSNFAEGVNSLMFMKNLTISGGFLLLAITGPGAYSIGRLLNKKW"
     gene            4351..5337
                     /gene="yqjG"
                     /locus_tag="ACHUQ7_002150"
     CDS             4351..5337
                     /gene="yqjG"
                     /locus_tag="ACHUQ7_002150"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HFR1135553.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5630..5998
                     /locus_tag="ACHUQ7_002151"
     CDS             5630..5998
                     /locus_tag="ACHUQ7_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFR1135554.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTIIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(6205..7101)
                     /gene="yhaJ"
                     /locus_tag="ACHUQ7_002152"
     CDS             complement(6205..7101)
                     /gene="yhaJ"
                     /locus_tag="ACHUQ7_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFR1135555.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFAGK"
     gene            7206..7907
                     /locus_tag="ACHUQ7_002153"
     CDS             7206..7907
                     /locus_tag="ACHUQ7_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFR1135556.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            7930..8094
                     /gene="yhaL"
                     /locus_tag="ACHUQ7_002154"
     CDS             7930..8094
                     /gene="yhaL"
                     /locus_tag="ACHUQ7_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFR1135557.1"
                     /translation="MSKKSTKKRQPAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(8306..9616)
                     /gene="cyuA"
                     /locus_tag="ACHUQ7_002155"
     CDS             complement(8306..9616)
                     /gene="cyuA"
                     /locus_tag="ACHUQ7_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708914.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFR1135558.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AITDAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTHQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITAIMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(9644..10975)
                     /locus_tag="ACHUQ7_002156"
     CDS             complement(9644..10975)
                     /locus_tag="ACHUQ7_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR1135559.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTDMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
     gene            complement(11250..12614)
                     /gene="tdcG"
                     /locus_tag="ACHUQ7_002157"
     CDS             complement(11250..12614)
                     /gene="tdcG"
                     /locus_tag="ACHUQ7_002157"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFR1135560.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPCRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(12686..13075)
                     /locus_tag="ACHUQ7_002158"
     CDS             complement(12686..13075)
                     /locus_tag="ACHUQ7_002158"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFR1135561.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(13089..15383)
                     /gene="tdcE"
                     /locus_tag="ACHUQ7_002159"
     CDS             complement(13089..15383)
                     /gene="tdcE"
                     /locus_tag="ACHUQ7_002159"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFR1135562.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKSEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(15417..16625)
                     /gene="tdcD"
                     /locus_tag="ACHUQ7_002160"
     CDS             complement(15417..16625)
                     /gene="tdcD"
                     /locus_tag="ACHUQ7_002160"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFR1135563.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(16651..17982)
                     /gene="tdcC"
                     /locus_tag="ACHUQ7_002161"
     CDS             complement(16651..17982)
                     /gene="tdcC"
                     /locus_tag="ACHUQ7_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFR1135564.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(18004..18993)
                     /gene="tdcB"
                     /locus_tag="ACHUQ7_002162"
     CDS             complement(18004..18993)
                     /gene="tdcB"
                     /locus_tag="ACHUQ7_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFR1135565.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLVDGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(19092..20030)
                     /gene="tdcA"
                     /locus_tag="ACHUQ7_002163"
     CDS             complement(19092..20030)
                     /gene="tdcA"
                     /locus_tag="ACHUQ7_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFR1135566.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
BASE COUNT         5215 a         5276 c         4814 g         4846 t
ORIGIN      
        1 caatcatcaa atttatatat gaccaatggc atacccatga ataaagtggt atgataactt
       61 cgtccgcatt ccactccggg agacgcatat ggaaatcact gaaccacgcc gtttgtatca
      121 acaacttgct gctgacctga aagagcgcat tgaacagggc gtctatctgg tgggtgataa
      181 actgcctgcc gaacgcttta ttgccgatga aaagaacgtc agccgaacgg tagttcgtga
      241 agccatcatc atgctggaag ttgaaggcta tgtggaggtg cgtaaaggtt caggtattca
      301 tgtggtttcc aaccagccgc gtcatcagca ggcggctgac aataatatgg aattcgccaa
      361 ttacggtccg tttgagttgc ttcaggctcg ccagctcatc gaaagtaata ttgccgagtt
      421 cgcggcaact caggtaacga aacaagacat catgaaactg atggccattc aggaacaggc
      481 gcgcggcgaa caatgcttcc gtgattccga gtgggattta cagttccaca ttcaggtagc
      541 cctggcgacg cagaactccg ccctggcggc catcgttgaa aaaatgtgga cccagcgtag
      601 ccataacccg tactggaaaa aactacacga acacattgat tcccgtaccg ttgataactg
      661 gtgtgatgac cacgatcaaa ttctcaaggc gttaattcgc aaagatcctc atgctgctaa
      721 gctggcaatg tggcagcatc tggaaaacac caaaatcatg ttatttaacg aaaccagcga
      781 cgatttcgag ttcaatgccg accgctatct gttcgccgaa aacccggtag tgcatctcga
      841 tacggccacc agcggcagca aatgaaattc ctgtccgaca ggcgctgcaa tgcgcctgtt
      901 tgtctcgtcg gtaagcgaaa agtataaagt gtcagcctgt gtaaatcctc tcgccaccct
      961 cccctgcaat cagcaaaatc agactccacg gacatgtaat tttgataacg aacaacgttg
     1021 acctttgtta caattagatt caatttgaat ttatgctttt gaatgctttc ttatctcacg
     1081 ttttaacagg gaatagttca ggctgtgttg atgtatcaaa cccgcagaac ataccaaaac
     1141 agcaataaca ttgcggtagt gcatctttta aaaccagcgt ggcgttaacc gattcaccag
     1201 gaataatgaa tggaactttt gacccaattg ctgcaagccc tgtgggcgca ggattttgaa
     1261 accctggcca atccatcgat gattggcatg ttgtattttg tcttgtttgt aattttgttc
     1321 cttgaaaatg gcttgcttcc ggcggccttt ttaccgggcg acagtttact ggtattggtc
     1381 ggcgtgttga ttgcgaaagg cgcgatgggt tacccgcaaa cgattctgct gctgaccgtt
     1441 gccgccagcc tcggttgctg ggtcagctat attcaggggc gatggctggg caatacccgc
     1501 accgtacaaa actggctatc tcatttaccc gcgcattatc atcaacgcgc acaccatctt
     1561 tttcataaac acggtttatc ggcgctgtta attggtcgct ttattgcatt tgtcagaaca
     1621 ctgctgccga cgattgccgg gttatcaggg ctgaataacg cgcgctttca gttcttcaac
     1681 tggatgagcg gtctgctgtg ggtattgatc ctgacaactc tgggttacat gctcggcaaa
     1741 acgccggtat ttttaaagta cgaggaccag ctgatgtcat gcctgatgct gctcccggtg
     1801 gtgctgctgg tgtttggcct ggcaggttct ctggtcgtgt tatggaaaaa gaaatatgga
     1861 aatcgggggt aagggatgca aatacctcgc atgtcgcttc gccagctagc ctggtccggc
     1921 actgttttac ttctggtcgg cacgctgttg ctggcctggt ccgcggttcg ccagcaagag
     1981 tctacgctgg cgattcgtgc cgttcatcaa ggcacaacga tgccagacgg tttttcgatc
     2041 tggcatcacc ttgacgctca tggcattcct ttcaaaagta tcacccccaa aaacgacact
     2101 ctgttaatta catttgactc cagcgaccag agcgccgccg caaaagcggt cctcgacaga
     2161 acattgcccc atggctacat cattgcgcag caggacaata acagtcaggc tatgcagtgg
     2221 ctgacccggt tacgggataa ctctcatcgc ttcggataac ttcctgttat ccgaaacata
     2281 tcactcactt tggtgatttc accgtaactg tctatgatta atgaggcggt ggttaacact
     2341 accgcttcgt tgtctggaca atcgttcctt tgtaataggt ccgaatcaca atggaaggtt
     2401 caagaatgaa ataccgcatc gctttagctg tttctctctt tgctcttagt gccggtagtt
     2461 atgccactac cctgtgtcag gaaaaggagc aaaatatcct taaggagatc agctatgccg
     2521 aaaaacacca aaaccagaat cgtattgacg gtctgaataa agccctgagt gaagtccggg
     2581 ccaactgttc agatagccag ctgcgtgccg atcatcacaa gaaaatcgca aagcagaaag
     2641 atgaggtggc ggaacgccag caagatttag ccgaggcgaa gcaaaaaggc gatgccgata
     2701 gaacggaaac tggcagaagc gcaggaagag ctgaaaaagc tggaagcgcg cgactactaa
     2761 ctcacaatag tcactactta ctcacctgga gaaaactatg tcgaaagaac acactacgga
     2821 acatctgcgt gctgagttga aatccctttc cgatacgctg gaagaggtgc ttagctcatc
     2881 tggcgagaag tcgaaagaag agttgagtaa gattcgtagc aaagcggagc aggcactgaa
     2941 acagagccgt tatcgcctgg gtgaaaccgg tgatgccatc gccaaacaaa cccgtgtcgc
     3001 ggcggcgcgt gctgatgagt atgtgcgtga aaatccgtgg accggcgtgg gaattggcgc
     3061 tgcaatcggt gtagtgctcg gcgttctgct gtcgcgtcgt taattatggc ggacactcat
     3121 cacgcgcaag ggcccggtaa aagcgttctg ggcatcgggc agcgaattgt ttccatcatg
     3181 gttgaaatgg tggaaacacg tctgcggctg gcggtggtgg agctggaaga ggaaaaagct
     3241 aatctctttc aacttttact gatgctgggc ctgacgatgc ttttcgctgc atttggtctt
     3301 atgagcctga tggtgctaat tatttgggcg gttgatccgc aatatcgcct gaatgcgatg
     3361 attgccacca ccgtggtgtt gctgctactg gcactgattg gcggtatctg gacgttacgt
     3421 aaatcgcgta aatccacctt gctgcgccat acacgccatg agctggcaaa cgatcggcaa
     3481 ctgctcgagg aggagtcccg tgagcagtaa agtcgaacgt gaacgacgta aggcgcaact
     3541 gcttagccag atccagcaac aacggctgga tatttccgcc agtagtcgtg aatggctgga
     3601 ggcaacaggc gcttacgatc gtcgctggaa tatgctgcta agtctgcgct cctgggcgct
     3661 ggttggcagt agagtgatgg cgatctggac gattcgccat cctaatatgc tggtccgctg
     3721 ggccagacgc ggttttggcg tatggagcgc ctggcgtctg gttaaaacga ccctcaagca
     3781 gcaacagctt cgcggttaac ttccctctgg ccggagcaat tccggcctta tccatcaaat
     3841 tttttgaata tttttgacag ttttccttgc taacaatcat cattcaccac gtttatgatt
     3901 ctctccatcg acagcaacga cgctaatagc gcgcccttgc acaaaaaaac aatcagcagc
     3961 ctgagtggcg cagtggagag tatgatgaaa aaattagaag atgttggtgt actggtagcg
     4021 cacattttaa tgccgattct gtttattacc gcaggctggg gaaaaatcac cgcttactct
     4081 ggtacccaac aatatatgga agcaatgggt gtcccgggtt ttatgctgct gacggcattt
     4141 ttatttcaca gcaactttgc tgaaggcgtc aactcgctga tgttcatgaa aaacctgaca
     4201 atttctggcg gattcctgct gctggcaatt accggtccgg gcgcgtatag catcggccgc
     4261 ctgctgaata aaaagtggta agcacgcgct atacttaacg ataaaaaacg aggaggaagc
     4321 tcctcgtttt tgctattgga ggagagaaaa atgggtcaac tgattgacgg cgtctggcat
     4381 gacacctggt acgataccaa atctaccggc ggtaaatttc aacgttcagc ttccgcattt
     4441 cgtaactggc tcactgccga tggcgcacct ggccccactg gcacaggcgg ttttatcgca
     4501 gagaaagatc gttatcatct ctatgtttca ctcgcctgcc cgtgggcgca ccgcacgctg
     4561 atcatgcgca aactcaaagg actggaaccg tttatttccg tttccgtagt gaacccgctg
     4621 atgctggaaa acggctggac ctttgatgac agttttccgg gagcaaccgg agatacgctc
     4681 tatcaacatg aatttctgta tcagctttat ctccacgccg atccacacta cagcggacga
     4741 gttactgttc ccgtgctgtg ggacaaaaag aaccacacca tcgtcagcaa cgaatcagca
     4801 gaaatcatcc gcatgttcaa taccgcgttt gatgcgctgg gcgcaaaagc gggtgattac
     4861 tacccaccag ccctgcaaac gaaaattgac gaacttaacg gctggattta tgacaccgtt
     4921 aacaacggcg tgtataaagc tggttttgcc accagccagc aagcctacga cgaggcggtg
     4981 gcgaaagtgt ttgaatcgct ggcgcgactg gagcagattt taggccagca tcgttacctg
     5041 accggcaacc agctaaccga agccgatatt cgcctgtgga ctacgctggt gcgttttgat
     5101 ccagtgtatg tgacccactt caagtgtgat aagcaccgca tcagcgatta cctgaatctg
     5161 tatggcttcc tgcgcgatat ctaccagatg ccggggatcg ccgaaactgt caatttcgat
     5221 cacatccgta atcattactt ccgcagccat aagaccatca accctacggg gattatttca
     5281 attggtccgt ggcaggatct cgatgaaccg catggacgag atgttcgctt cggttaaaaa
     5341 aaggcacctt acgaggtgcc tctgataatt agctaagtgc atgttgttca tgtcggatgc
     5401 ggcgtgaacg ccttatcaaa cctacaaagc gtttatattc aatatgttac aatgaccgcg
     5461 taggccagat aagcgtagcg catctggcga ttttgcgctt gccatcagtc ttagcgcgcc
     5521 ttcacggcac tttattaatt ccttgaacga atatttactg ccatttcatt cacgtctatt
     5581 cttaatttgc tgctttaaaa acaagtgatt gagcaaaatt gaggcaaaaa tggactggta
     5641 tctgaaagta ctaaaaaatt atgtcggttt ccggggtcgt gcgcggcgca aagagtactg
     5701 gatgtttatt ctggtcaaca tcatctttac gttcgtgctg gggctgctgg ataaaatgtt
     5761 aggctggcaa cgcgctggtg gcgaaggcat cctgacgata atctacggta ttctggtgtt
     5821 tttaccgtgg tgggcggttc agttccgccg cctgcacgac accgaccgct cggcgtggtg
     5881 ggcactgcta ttcttaatcc cgtttatcgg ctggcttatt atcatcgtct ttaactgcca
     5941 ggcgggtacg ccaggcgaaa accgctttgg accagatcca aaactggaac aagaataatg
     6001 attaagcacc ttgtaaaagg tgcttcaaat tgttgacaaa gtgcgctttg tttatgccgg
     6061 atgcggcgta aacgccttat ccagcctaca aattgccaaa attcaatata ttgcagtgac
     6121 tgcgtaggcc tgataagcgt agcgcatcag gcagttttgc gtttgtcagc agtcagaagc
     6181 accttgtgaa aggtgcttaa tagattattt tccagcgaac aactttggaa tttcccgcag
     6241 acaccaggat ttcgcttccc ccatactgtc acgacgccag gccataataa tatcgatctc
     6301 gctggtcgat tccgggctga cgacacgcaa ccgcccttcc gcaatatctt tttcgaccat
     6361 cggatacggc atcgtcgcca cgccaagccc cgccagtaat gcctgacgtt tatcttcaat
     6421 cgtgctcacc gttaagcgcg gctgtttgtc cagcagctgt acggtcaaca ccgggcgctc
     6481 acgagcggta tccgctaccg caattccacg atatttcaca cgcgtcactt cagataacgg
     6541 ttccggctcc tgatgaatcg ggtgatcagg cgcggcaacg tagacgttca ttaacgtata
     6601 gagcttgcgc gagttgatct ccgacgagga acgaaaatgc atatccggcg cgataacgat
     6661 atccgcccgc ccctgctcca gccgttccca cgcccccgcc agcacttctg tgatgattgc
     6721 cagttgggta ttggcttttg ccgccagttt gtcgattaac gggaaaaagg caggtgtcgg
     6781 taccagcgct tcggtcacaa tggtgagatg cgtttcccaa ccgcgcgcga gagcttccgc
     6841 atcagtagtc agtttatctg cggcttccag caaaacgcgc cctcgctcca gcaacatcct
     6901 cccgacattg gtgaatttgg tgcgatggcc cgagcggtca aacagcacca catccagctc
     6961 ttcttccagt ttttgcatgg tgtagctaag tgcggaaggc acgcgtccca gctcatccgc
     7021 tgccgccgca aaactgcccc ggcgatcgat cgcatccata acccgtagtg cttccagcgt
     7081 taatgccctt tctttggcca tttcgttctc attcaggaaa tttgaacata ccgggcagaa
     7141 tatctggcta acaatgtagc gtccagcccc ttaccataaa aggaagtaaa gagaggtcaa
     7201 gaattatgat tactacccga actgccaggc agtgtggaca agcagactac ggatggttgc
     7261 aggcccggta tactttttcc tttggacact acttcgaccc gaaattgtta ggctatgcct
     7321 ccctgcgtgt gcttaaccag gaagtgctgg ccccaggtgc cgcctttcag ccgcgaacct
     7381 atcccaaagt cgatatttta aatgtgattc tggatggaga agcagagtat cgcgacagcg
     7441 aaggcaatca tgttcaggcc agcgccggag aggcgttgct gctctctacc cagccgggtg
     7501 tcagctatag cgaacacaat ctcagcaaag acaaaccgtt aacgcgaatg cagctttggc
     7561 tggatacctg cccgcagcga gagaatccgc tgattcaaaa gctggcgctt aatatggaca
     7621 agcagcaatt aatcgcctcg ccagagggtg cgatgggaag cctgcaatta cgccaacaag
     7681 tgtggctgca ccatatcgtg ctcgacaaag gcgaaagtgc gaatttccag ttgcatgggc
     7741 cacgcgcgta tttgcaatcg attcacggga aatttcatgc gcttacgcat catgaagaga
     7801 aagcggcgct aacctgcggt gatggggcgt ttattcgtga cgaggctaac attacgctgg
     7861 ttgccgattc cccactgcgc gctttgctga tagatttgcc tgtctagttg tttttacagg
     7921 gagatgatga tgagtaaaaa atcgaccaaa aagcgccagc cagcgaagcc cgtggtggcg
     7981 aaagaacctg ctcgcaccgc caaaaatttt ggctatgaag agatgttgag cgagctggaa
     8041 gctatcgtcg cggatgctga aacgcgttta gccgaggatg aagctaccgc gtaaagacag
     8101 gttgccggat gcggcgtaaa cgccttatcc ggcctacaaa acacaacaac atcaacaaat
     8161 taaacacccc gtaggccgga taagatgcgc cagcatcgca tccggcatca ggtacaaaac
     8221 acaacaacat caacaagttg agcaccccgt aggccggata aggtgcgcca gcatcgcatc
     8281 cggcacaata ctcaaaacct ggcgtttatc tggccttgct cgccataatc tcgataatct
     8341 gccgatccgt ttgctgcatc gaatggcttg ctaacgcaca caggttggca atcgactgct
     8401 caacatcatg cgccacgatc ccttcattgc cggtcacggc ggtatcatcc agcgccatta
     8461 acaccgcttt ccacgcagcc gaagcactgg tcgaaacctt catcgcgcag ctgttcgacg
     8521 caccatcgca aatcatgccg ctgacatcgc cgatcatact gctgatcgcc atcgagatag
     8581 tttcataacg cccatccacc agccatgcca tcccggcggc ggcccccatt gctgcggtcg
     8641 ttgcggcaca aagtgcagac aaacgcggta actggttatg gatgtaaatt gcgctcaaat
     8701 gagaaagcat cagcgcacgc gccagtcgtt catcatccgc tccgaagtgt tctgctacaa
     8761 ccaccacagg cattattgca gtgatcccct ggttacccga gccggagtta ctcatagccg
     8821 gaagcgtagc gccgcccata cgcgcatcgg atgccgcgct ggtacgaatc acaatggatg
     8881 aagagagatc tttcgccagc aaaccgcgct cgcactgttt ttccagcgtc gcgccaatat
     8941 gcagccccca cttaccgctc aaaccttcct gcgataacgc acaatttagc ttcgcggaat
     9001 cgagaataaa gcggatcgcc gcaaatggga cttcattgac gaacttcagg atctcagcca
     9061 gcgtcgttct ggaaagcacc gtcagcggcg actcttgctc gccctctgcc acacacgcct
     9121 ggtgggtaaa caccacacca ttgtgcgtct caatatgcac aatgttggta tgcccgccga
     9181 cgatggtgac acacgcccac ttctcaccgt tccagacttt ggcgcgtgag aagaggattt
     9241 cattgcaagg ttcctggatc ttaacagaga ctttccccgc cgccagcagt gctttggcat
     9301 cggtaattgc ctgcgcagtt gcgtctttca gcacttccag cccggcgttg gcatttccac
     9361 ctaacgcccc cagcgccgcc gcaatcggca gccccaccat tcccgtgccg ggaacggtga
     9421 cgcccagacc gttcttcatc agatttggcg aaacccaggc ttctacacgt tcaaccggac
     9481 cttccagttc tgccgcagca accgccgccg ccagcgccag tgaaatcggt tcagtacatc
     9541 ccagcgccgg ttttacttcc tcctgaacgg cgaggatgta acgctgccat aacggattta
     9601 aagtcgaatc aaacatatta aaaaccttaa aatttcaggt aaatcaagaa aatgccagga
     9661 acagagaaac acaaagcaac aaaccagtga cgataatcag gtacagagac atccctttgt
     9721 atttgtgcaa tgccggtact ttgtaaacca gccacgccgg gatcaggcac cctaccatgc
     9781 cgaaaatcgg gctacagata gaggtgaagc tcaacaccgg tgcgttcagt acgatggcgc
     9841 tccaggccag caaaatggcg aaaatcatga tgccgcgctg aacaagattt tcgttaatct
     9901 tctcggcagg catcttgcga cgcaggatgt tcattacgat cccttgcgtt gcttcgcgaa
     9961 agcctaaata gacgccaaag aatgcagtca tgacagcaaa gatattgaga atgacgctga
    10021 ccactttcac ccacgctgcg ccgtcaccgc taataaactg tgcggcaatc gccagcgcgg
    10081 aaatattctg ctcgtaggct tttaccgctt cgtcatgtcc catcgccagc gtgaacgaca
    10141 cggcgtagaa aaagacggtg acaaacaaaa tgccaaacgc gatattcatt gcccgcaatg
    10201 ctttatgccg cgccacttca attgattttt cccgcgagcg ataagagatc accatcgggc
    10261 ttaacgtctg gataaacaga atcgacgtca gggtaaacgg cagcgtaata atggcgtttt
    10321 tcaccagcag ccccagcggc ggtagcgaac cgacgttata cagatgccac attcccacca
    10381 tcgacacgcc cagcgcagcg actaccagca gcttggtcag caccatgtcg gtcgaaattt
    10441 tgaataacaa tttctcgccg cgtgaagaga tcgccaccag aatgcaaatc agtaccagac
    10501 cataaaaggg actgtctgac agcaaccctt ccgtcacgcc gaaggtatgc agataggaag
    10561 cactatcgtt ggtgatggcg gtggaataga cgaacatcca aatcaccagc attacgaaat
    10621 agagcgcacc taacaggatg ccccagtttt tacctaaata accgctaatg acactcgggt
    10681 aatctttaca ttctggtgat tctgccagcg tattaataaa caaccgctga aacagataca
    10741 ttgccgggta accaatcacc gatgagagca aaaataccca caatcccatc aaaccgacct
    10801 gcaccgggag aaaaacaatc cccgcgccaa tcgccatccc gatactcata atcacccagc
    10861 cggtgtcagt actgtcgaat ttgatcgctt ctcgccactc gctctcactc attccagcac
    10921 gacccgccgg ggtcgaagcg tctgcaatga cgcctttatt cgatgcaatt tccataattt
    10981 ctcgctcaat attttgtagg gcttattatt tttttccgag ccgcatcaag gcgatatgcg
    11041 gtctacgttt ttgcaggcga gttattagaa taaagaaatg atacgcgcga ggtcagagaa
    11101 aatcttcaca atcaagactt taaaataacg gagatggata agaattttct actaaattaa
    11161 tcacaagaga atattcattc tctatttgcg gcagatcaca aaaaaaaggt gcacatttgt
    11221 gcacccaagg atgaaagctg acagcaatgt cagccgcaga ccactttaat ggccagtcct
    11281 ccgcgtgatg tttcgcggta tttatcgttc atatctttgc cggtttcata catcgtctcg
    11341 atcactttat cgagtgaaac acgcggtgcc gaggtgcggc gcatcgccat ccgcgcggcg
    11401 tttactgctt tcacggcatt aatggcatta cgttcaatgc acgggatttg tacctgtccg
    11461 gcaaccggat cgcaggtcag cccaaggtta tgctccatcg cgatttccgc cgcattgcat
    11521 acctgcgccg gactgccgcc cagtagttca gttaaccctg ccgctgccat tgaacaggcc
    11581 acgccaatct ccccctgaca gccgacctcc gcgccagaga tggaggcgtt cattttatac
    11641 agcgcgccaa tagcccccgc tgccagaaaa tagcgggcaa ttgaccgctc gtttaccgga
    11701 cgacggaact tatcgtaata agccagcact gccggaataa tgccgcacgc accgttagtc
    11761 ggtgccgtta ccacgcgccc gccagctgcg ttttcttcac taaccgccag cgcgtacatg
    11821 ttgatccagt cgatgacatt catcggatcg ttagagatgt tatcgctaga aaccagctga
    11881 cgacgcagcg ctacggcacg acacggcaca ttgagcggac caggcagcac gccttcagtg
    11941 ttcatcccgc gttcaatacc gtcatgcatc acttgccaga tacgggcaaa ccctgcgtca
    12001 atttccgctt tgctgcgcag cgccagctcg ttgtgcatca tcaggccaga tatagacagg
    12061 ccgttgtaat cacacatttt cagcagttca cctgctgagt ggaaatcgta aggtacggac
    12121 gtttcgacat cgtgcgacag gccgaagtgt tcttcttcga caataaaccc gccgccgaca
    12181 gagtaatagg ttttacttaa tagcgcttcc tgtcccttcc aggcagtgat ccgcattccg
    12241 ttctcatggc gaggcaacat ttcgggatgg aagataatgt tctttgctac aggaaaatca
    12301 acaatatgcg caccagatgc cactggcagc cgcccgctgc gcgttactaa ctctataaat
    12361 gcagggatct catcaatgac aacatcctgc ggactgtttc ctgccagtcc catgataatg
    12421 gcgacatccg tggcatggcc tttgcccgtc agtgacaacg acccgtacag atcgaccaca
    12481 atatggctcg tcgcggttaa taagccgcta ctttccagcc gatcaataaa actttttccg
    12541 gcattcattg gccccacggt atgcgaactg gagggaccaa tcccaatttt gaaaatatcg
    12601 aatgcactaa tcatatccac accctcggat tgccgttcag tgaagtggag cggaacgacc
    12661 ttgcgaccgt cccgctcacg aggctttacg cactacgtac tgcgatagct tcaatttcca
    12721 gcttcacatc tttcggcaaa cgcgcaacct gcacacagct ccgggtcggg taggtcgcct
    12781 gatgctcatc gaagaactgc ttatagactt cattgatggt ggcaaaatca ttcagatcgg
    12841 tgatgaacac ggtcatcttg atgatatcgc ccacgctcag cccggcagca accacgatcg
    12901 ctttgacgtt ttcgaggctt aaacgcgcct gatcttgcac atcagccggg atctcaccgg
    12961 tctgcgggca aaccggaatt tgcccggagg tgaagaccat gctgcctaaa tcaacgccct
    13021 gaacataagg gccgattgcg cctggggcac gttgcgtttc gataatcttt ttcatacatc
    13081 ctccggcgtc agagcgcctg ggtaaaagta cgtgaaataa catcctgttg ctgttcacgg
    13141 gtcagtgcgt tgaagcgcac ggcgtagcca gagacacgga ttgtcaggtt aggatatttt
    13201 tccgggtgct cgatggcatc cagcagcatt tcccgattca ttacgttgac gttgaggtgt
    13261 tgaccgcctt cgacatccgc ttcgtggtgg aaatacccat ccagcaggcc gacaaggttg
    13321 gttttacgta ctggatcttc tttgcccagc gccgcaggaa cgattgagaa ggtgtacgag
    13381 atcccatctt tggcgtaggt gaacggcagt ttcgccaccg acgtcaatga ggccacggca
    13441 cctttgcggt cacgaccatg catcgggtta gcgcccggcg cgaacggtgt tccggcgcga
    13501 cgaccgtccg gcgtattacc ggttttctgg ccgtacacca cgttagaagt gatagtcaga
    13561 atcgactggg tagggacggc gttgcgatag gttggcagcg ctttaatttt cttcataaag
    13621 cgttcaacca ggtcgcaggc aatgctgtct acgcgctcgt cgttgttgcc gtactgcgga
    13681 tattcaccgt cgatttcaaa gtccaccgcc aggccgtttt cgtcacggat tggtttcacg
    13741 cgggcatatt tgatggcaga cagggagtcc gtcgccaccg acaggcccgc gatgccgcat
    13801 gccatagtgc gatagacatc acgatcgtgc agcgccatca gcgaagcttc gtagctgtac
    13861 ttgtcgtgca tgtagtgaat gatattcagc gcgctgatgt actgcaccgc cagccagtcc
    13921 atgaagtgat cgaggctgtc catcactttg tcgtaatcca gcacgtcgtc catcagcggt
    13981 gctgttttcg gcccgacctg aatcttcagc ttctcgtcca ccccgccgtt aattgcgtag
    14041 agcagcgttt tcgccaggtt agcgcgtgca ccaaagaact gcatttgctt accaatcacc
    14101 attgggctga cgcagcaggc aatcgcgtaa tcgtcgctgt tgaagtcagt acgcatcaga
    14161 tcgtcatttt catactgcaa ggaagaggtg acgatcgaca cctgcgcggc atattttttg
    14221 aaggcaatcg gtaattcttc cgaccaaaga atggtcaggt taggttccgg tgccggcccc
    14281 atagtgtgca gggtgtgcaa atagcggaag gagtttttgg tcaccagcgt acgaccgtcc
    14341 agccccatcc cgccgatcac ttccgtcgcc cagattgggt cgccggagaa cagcgaatca
    14401 aattccggtg tacgcaggaa gcgtaccata cggatcttca tgatgaagtg atcgatcagt
    14461 tcctgtgcct gctgctcatt gaggacgcca gctttaaagt cgcgctcaat gtagatatcc
    14521 aggaacgatg ccgtgcgccc cagcgacatc gcgccgccat tttgcgattt cactgccgcc
    14581 agataagcga agtagagcca ctgcaccgct tcctgtgcat tctgcgccgg gcgagagata
    14641 tcaaagccat atttcgccgc catttcctga atctgcaaca gcgcatgacg atgctctgcc
    14701 agctcctcac gcagacggat ggtggcttcc agatcctcgc ctttttccag acgagactgg
    14761 agatcggcaa attgcagttc gcgttcacgc accagataac tgatgccata cagcgctacg
    14821 cggcgatagt caccgataat gcgcccacga ccatagccat ctggtaaacc ggtcagcacg
    14881 ccagatttac ggcagcgcag catatccggt gagtaaacat caaatacgcc ctggttatgg
    14941 gttttacgca gatcggtaaa cagatattca aattcactgt ccatttcacg gccataggcg
    15001 tggaatgaac ttttaatcat attaatgcca ccgaacgggt gtagcgcacg tttcaacggc
    15061 gcatccgttt gcaggccaac aattttttcc agcggctggt taatatatcc cgcatcatga
    15121 gcggtaattg tggtggcaat attggtatcg aaatcaaccg gcgcgtgggt tgcgttttcg
    15181 atacggatgc cttccattac cttttcccac aattccgtgg tggcaggcgt cgcttcggcg
    15241 agaaaagatt catcgccttc atacggtgta tagttatgtt gaataaaatc gcggacatta
    15301 atttcgcttt tccagtccgt acctttaaag ccaagccatg cgtcagcgta cagcttatcg
    15361 ctggtatcaa tatctacctt catgaaaaat aatctctcta caatacttca actaaattat
    15421 gcaaattctg cgggcgcgtt aactttacct aaatgaatgg catccaaagc aatcattttt
    15481 tcttcgttag tcggaataac gacgcaaatg acatgcgcat tttcactgga aacaattcgc
    15541 tcgccaaaag agttagaacg attattcatt tctgtatcaa tctctacgcc taatacagcc
    15601 aaatgttcca tgaccagacg acgaattaaa cttgaattct ctcctattcc gccggtgaat
    15661 ataattccat ccagccgacg taatgaagct gcatgtccgg caatatgacg ggcaattcgg
    15721 tgaacaaagg ttttaattgc cagttgcgcg cgttcgtgac cttcatgcca ggctttttcc
    15781 agaacacgta aatccgaaga aagaccggaa atacctaata atcccgactc tttattcact
    15841 acgcgttcca ggtcacccag gctctggttg gtttggctgg cgacccagga catcgcacca
    15901 aagtcgacat cgccactgcg ggtacccatc atcaagcctt ccagcggcgt cattcccatt
    15961 gaggtgtcaa cactctgacc gttgcgaact gcgcagattg acgcgccatt gccaagatgc
    16021 gccacaacca ggccggagtc atcttccgcc agattcagca gcgaatgtgc gcgctgggaa
    16081 acatagcggt gcgacgtgcc gtggaaacca taacggcgta cacctaactc ttcataatat
    16141 ttccacggca ggccgtataa ataagcttcc ggagccatcg tctggtggaa actggtatca
    16201 aataccgcca cctgagttac gcccggaaat aattgctgcg ccgattcaat accacttaaa
    16261 tttgcgtaat tatgcagggg tgccagtgga gaaacgcgac ggatattatc aatgacttca
    16321 tcggtaataa tggcggactc ggtaaaaata ctgccgccgt gagcgatgcg gtggccaatt
    16381 aaggccacgc tgtcatttaa attccgtttt tccagttcaa atgcaattgc cttcaatgca
    16441 ccttcgtagc tgtggtgagc cagcggtgct ggctctcccc catttacgga taagaatgca
    16501 ttttccgagt taataccgtc ggcaatacct gacattaata cttcacaatc gctggcatcg
    16561 agtacggaaa acttaatcga agacgaacca cagttaataa ccaaaacaac cggaaattca
    16621 ttcatctctt ttctcatcct gagttacgga ttaaaacagt ttgtatacga tgttcaggat
    16681 ggtcagcaga ccaatcacgg taacaaacac gttatccaga cgaccacggt atttcgccag
    16741 agacggcgct ttacggatag catacatcgg caacaggcac agcagggatg cgataatcgg
    16801 tgcgcccatg gcttcaatca ggtcgaggat gttcgggttg gcgtaggcaa caacccaggt
    16861 ggagcccatg atgaagatca tgctgagagt attcagttta cccagcgaca ctttggtttt
    16921 gtcaccttta taaccgaact tcagaatcag accattcaag ccttccagcg tccccagata
    16981 gtgaccgaag aaagatttga agatagccac gagtgcgatg atggaagccg catattccag
    17041 tgtaatcgcg aacgttgttt tcgtaccggt catggacgca aagtggttag ccagataaga
    17101 aagcactgga atattctgcg ctttggcttc cgccatgttg gccggagaca gagtaaacag
    17161 gcagctaaag gcaaagaaca tcaccactgc aaccatcagc atgctggcac gagaaatgat
    17221 ttgggaacat ttacgttcgg tgaagtcgcg accgaagtct ttctcatact cttcacgttt
    17281 agaaaccacg aaggaagaga cgattggcga gaagttaaag gagaaaacca tgatggaaat
    17341 ccccagccag acagtgatca ggataccgtc atgaccggtt aacgacagcg aaccgaggtc
    17401 aacctggtcg ataactgcag agttccagta agggatcagc gacagagaaa tcagcaccag
    17461 gctggcgata aacggccata ccaggtagct catcacttta accatcagat ccttaccaaa
    17521 ccagatgacg aaagccatca gcagcaacag gaacagcgcc acaaagccgc gattcagcgg
    17581 tgcaaatccg agctggtttt cccagaacgt cataaaggta ttggtaatag taacgccata
    17641 aatccacagc agtgggcaaa tcgcgaagaa gtacaggaac gtgataacca cgccgccagt
    17701 tttaccaaaa tgctcttcca ccgtttccgt aatgttgccg gaagggttag agccagaaag
    17761 acacagacgc gccagcgccc ggtggcaata aaatgcgatg gggtatgcca ataccaacat
    17821 cagaagaatc gggatcagtc cgccaaaacc tgcgcggata gggaagaaca gcaccccggc
    17881 gccgattgcc gtaccaaaca agcctaacgt ccatgtggta tctgatttac gccaggacga
    17941 ttgttttgtc tggctggata caatgctatc tgaagtactc atatcctatc ctcaacgaat
    18001 taattaagcg tcaacgaaac cggtgatttg agagacgcga gaaagatcga tattgccgcc
    18061 ggaaataata ctgacggttt ttctattttg aatatattgg tctaatttac cgcttaataa
    18121 tgcagcacat gccagagcgc ctgcgccttc ggtgacgact ttattgcgct gaattaaggc
    18181 aatcatactg tttctgattt cgtcttcgct gaccagcacg atgtcatcga ctaattcacg
    18241 aacgatttcg taagttaaat tacccgggcg ggagacatca caaccatcca ccagggtgcc
    18301 ggtagttcgg tgcgtggtta tttctccgga gtggaaagaa gccgccatgc cgtgaacgtt
    18361 ttctgactgt acgccaataa cacgaatggt cgggttaata gatttaattg ccaccgcaat
    18421 accagcaatt aaaccaccac caccaattgg cacaatcacg ttatcgacat catagagatc
    18481 ttccataatt tccagaccaa tcgttccctg gccagcaatc actttcggat catcgtaagg
    18541 tgggataaaa atacgacctt ccatttcgac aatttcgctc actttagcga tagtgtcgtt
    18601 gaagttatca ccatgcagaa cgacttctgc ggagtagtcg cacgttgccg ctactttgga
    18661 ttttggcgca ccttttggca tcaccacttt accgtcgata cccagcatcg cgcaggagag
    18721 cgaaacccct tgcgcatggt tgcccgcaga acaggccacc acgcctttgc gtttttccgc
    18781 atcggtcagt gaacttaatt tattaaatgc gccacgaatt ttaaatgaac ccgtacgctg
    18841 catattttca aacttcagga atatttcacc tttgcaacgt tcactaaaat agttggagcg
    18901 aggcatgcct gttttataaa ttcgcccagc cagtcgttgt ttcgcttcaa taatatcatc
    18961 aatagcaacc ggcagatcgt atgtaatatg cattataaaa cctcttcgcc tgaattaaat
    19021 atgtaggtaa ccgtaaccga cacctgcaaa acagacaggt ggattattta tgtttaaata
    19081 aaaacaaaca actaaccaac ttctattaat tgccttcgtc tacacccatt ataagatgaa
    19141 tactctttgg ctaattccac caaaaccgat gctgcttttt taatacgata atttttcgac
    19201 cataccgcgg catattgtgc cacaggtaat gtttcttcaa ccggaatagt aataaattga
    19261 ttagaaccaa aaggtgacgt catatcacaa ggaattacag ttaagaaatc agcattgaga
    19321 acaagattat aaattgtcac gactgagtcg gttttaacga tgttttcaat actgatgcca
    19381 tttctttgta acgtagtaag cagttcgctg tagtacccca tattagtttg tggcaacacc
    19441 cactgttcgt tcttcaacga ctccagcgtg gtggtgccgg tgcatgttcg ggacttactg
    19501 gctaccagca caaactcgga ctcgaacagc ggctcaacat gtaaatcctg aagcttcatt
    19561 tctgcactta acgtaccaat cgcaaaatcc agtcgaccgt cgcggattgc cggtaagaac
    19621 gaagacagtt gcgcttcata catagaaacc tgcgctttcg ggaacacctc tttgaacttg
    19681 ttgatcatcc ctgacataaa agtaaaacca atcaatgaag gaaaaccaaa tgagacttcc
    19741 accaccgctt cagaagacat accgcttatc tcattaacca tatttttcat ttcacgggta
    19801 atggattcgg aacgggagag taacaattga ccggcaggtg ttaatgttac accggtattt
    19861 ttccgcacca ctaattccac accaaaataa tcttcaatat cgttaatgat tttactgacg
    19921 gccggttgag ttaaccctaa ttcttttgca gccgagccga tagagccact tctaatgact
    19981 tcctgaaaga ctaccaggtg ctgcgttttc ggaagaagaa tagtgctcat aacgacctac
    20041 gttaattacc tcattgacgg catgaagtgt atcaaaatga aataaacagg atatgtgcga
    20101 ccactcacaa attaactttc attactttcc aaggtatcgt tattacaatt a
//
LOCUS       DAZPCS010000062        20030 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZPCS010000062 DAZPCS010000000
VERSION     DAZPCS010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20030
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.062"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            251..1345
                     /gene="gcvT"
                     /locus_tag="ACHUQ7_002164"
     CDS             251..1345
                     /gene="gcvT"
                     /locus_tag="ACHUQ7_002164"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFR1135567.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            1369..1758
                     /gene="gcvH"
                     /locus_tag="ACHUQ7_002165"
     CDS             1369..1758
                     /gene="gcvH"
                     /locus_tag="ACHUQ7_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFR1135568.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            1877..4750
                     /gene="gcvP"
                     /locus_tag="ACHUQ7_002166"
     CDS             1877..4750
                     /gene="gcvP"
                     /locus_tag="ACHUQ7_002166"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFR1135569.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWTEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            5017..5760
                     /locus_tag="ACHUQ7_002167"
     CDS             5017..5760
                     /locus_tag="ACHUQ7_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR1135570.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LIMQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDSLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(5817..7250)
                     /gene="bglA"
                     /locus_tag="ACHUQ7_002168"
     CDS             complement(5817..7250)
                     /gene="bglA"
                     /locus_tag="ACHUQ7_002168"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFR1135571.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTCGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKFVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLCGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            7295..7605
                     /gene="yqfB"
                     /locus_tag="ACHUQ7_002169"
                     /pseudo
     CDS             7295..7605
                     /gene="yqfB"
                     /locus_tag="ACHUQ7_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
     gene            7769..8428
                     /locus_tag="ACHUQ7_002170"
     CDS             7769..8428
                     /locus_tag="ACHUQ7_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFR1135572.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(8624..9604)
                     /gene="ygfZ"
                     /locus_tag="ACHUQ7_002171"
     CDS             complement(8624..9604)
                     /gene="ygfZ"
                     /locus_tag="ACHUQ7_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFR1135573.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            9847..10113
                     /gene="sdhE"
                     /locus_tag="ACHUQ7_002172"
     CDS             9847..10113
                     /gene="sdhE"
                     /locus_tag="ACHUQ7_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFR1135574.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMSFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            10094..10501
                     /locus_tag="ACHUQ7_002173"
     CDS             10094..10501
                     /locus_tag="ACHUQ7_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFR1135575.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(10541..11062)
                     /gene="fldB"
                     /locus_tag="ACHUQ7_002174"
     CDS             complement(10541..11062)
                     /gene="fldB"
                     /locus_tag="ACHUQ7_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFR1135576.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIIALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            11174..12070
                     /gene="xerD"
                     /locus_tag="ACHUQ7_002175"
     CDS             11174..12070
                     /gene="xerD"
                     /locus_tag="ACHUQ7_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFR1135577.1"
                     /translation="MKQELARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            12095..12805
                     /gene="dsbC"
                     /locus_tag="ACHUQ7_002176"
     CDS             12095..12805
                     /gene="dsbC"
                     /locus_tag="ACHUQ7_002176"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFR1135578.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            12811..14544
                     /gene="recJ"
                     /locus_tag="ACHUQ7_002177"
     CDS             12811..14544
                     /gene="recJ"
                     /locus_tag="ACHUQ7_002177"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFR1135579.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            14635..15733
                     /gene="prfB"
                     /locus_tag="ACHUQ7_002178"
     CDS             join(14635..14709,14711..15733)
                     /gene="prfB"
                     /locus_tag="ACHUQ7_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFR1135580.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            15743..17260
                     /gene="lysS"
                     /locus_tag="ACHUQ7_002179"
     CDS             15743..17260
                     /gene="lysS"
                     /locus_tag="ACHUQ7_002179"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFR1135581.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(17303..17851)
                     /gene="idi"
                     /locus_tag="ACHUQ7_002180"
     CDS             complement(17303..17851)
                     /gene="idi"
                     /locus_tag="ACHUQ7_002180"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFR1135582.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPRGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            17906..17977
                     /locus_tag="ACHUQ7_002181"
     CDS             17906..17977
                     /locus_tag="ACHUQ7_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1135583.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            complement(17974..18099)
                     /gene="yqfG"
                     /locus_tag="ACHUQ7_002182"
     CDS             complement(17974..18099)
                     /gene="yqfG"
                     /locus_tag="ACHUQ7_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFR1135584.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(18101..19549)
                     /gene="uacT"
                     /locus_tag="ACHUQ7_002183"
     CDS             complement(18101..19549)
                     /gene="uacT"
                     /locus_tag="ACHUQ7_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFR1135585.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIICGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVMRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLEVRTVRMWLLMRKLKKNEHGE"
BASE COUNT         4938 a         5004 c         5374 g         4714 t
ORIGIN      
        1 tcatcacatc atctttgtat agaaattggt gtattttttg gttttttatt ctgtcgcgat
       61 ttttgcattt tttaaccata agctaatgtg atgatcaatt ttaccttatg gttaacagtc
      121 tgtttcggtg gtaagttcag gcaaaagaga acgattgcgt tggggaccgg gagtggctcc
      181 gatgctgggt tttgtggtga taatttcacc atgaaaaagt tgtcagcccc gcttattcaa
      241 tgaggacaag atggcacaac agactccttt gtacgaacaa cacacgcttt gcggcgctcg
      301 catggtggat ttccacggct ggatgatgcc gctgcattac ggttcgcaaa tcgacgaaca
      361 tcatgcggta cgtaccgatg ccggaatgtt tgatgtgtcg catatgacca tcgtcgatct
      421 ccgcggcagc cgcacccggg agtttctgcg ttatctgctg gcgaacgatg tggcgaagct
      481 caccaaaagc ggcaaagccc tttactcggg gatgttgaat gcctctggcg gtgtgataga
      541 tgatctcatc gtctactact ttactgaaga tttcttccgc ctcgttgtta actccgccac
      601 ccgcgaaaaa gacctctcct ggattaccca acacgctgaa cctttcggca tcgaaattac
      661 cgttcgtgat gacctttcca tgattgccgt gcaagggccg aatgctcagg caaaagctgc
      721 cacactgttt aatgatgctc agcgtcaggc ggtggaaggg atgaaaccgt tctttggcgt
      781 gcaggtgggc gatctgttta ttgccaccac tggttatacc ggtgaagcgg gctatgaaat
      841 tgcgctgccc aatgaaaaag cggccgattt ctggcgtgcg ctggtggaag cgggcgttaa
      901 gccctgcggt ctgggggcgc gtgacacgct gcgtctggaa gcgggcatga atctttatgg
      961 tcaggagatg gacgaaacta tttctccttt agccgccaac atgggctgga ctatcgcctg
     1021 ggaaccgaca gatcgtgact ttatcggtcg tgaagccctg gaagtgcagc gtgagcatgg
     1081 tacagaaaaa ctggttggtc tggtgatgac cgaaaaaggc gtgctgcgta atgaactgcc
     1141 ggtacgcttt accgatgcgc agggcaacca gcatgaaggc attatcacca gcggtacttt
     1201 ctccccgacg ctgggttaca gcattgcgct ggcgcgcgtg ccggaaggta ttggcgaaac
     1261 ggcgattgtg caaattcgca accgtgaaat gccggttaaa gtgacaaaac ctgtttttgt
     1321 gcgtaacggc aaagccgtcg cgtgatttac ttttttggag attgattgat gagcaacgta
     1381 ccagcagaac tgaaatacag caaagaacac gaatggctgc gtaaagaagc cgacggcact
     1441 tacaccgttg gtattaccga acatgctcag gagctgttag gcgatatggt gtttgttgac
     1501 ctgccggaag tgggcgcaac ggttagcgcg ggcgatgact gcgcggttgc cgaatcggta
     1561 aaagcggcgt cagacattta tgcgccagta agcggtgaaa tcgtggcggt aaacgacgca
     1621 ctgagcgatt ccccggaact ggtgaacagc gaaccgtatg caggcggctg gatctttaaa
     1681 atcaaagcca gcgatgaaag cgaactggaa tcactgctgg atgcgaccgc atacgaagca
     1741 ttgttagaag atgagtaacg gctttattcc tcttctgcgg gagaggatca gggtgaggaa
     1801 aatttatgcc tcaccctcac tctcttcgta aggagagagg ttcacaattc actgcacgtt
     1861 tcaggaacca tcgctcatga cacagacgtt aagccagctt gaaaacagcg gcgcttttat
     1921 tgaacgccat atcggaccgg acgccgcgca acagcaagaa atgctgaatg ccgttggtgc
     1981 acaatcgtta aacgcgctga ccggccagat tgtgccgaaa gatattcagc ttgcgacacc
     2041 accgcaggtt ggcgcaccgg cgaccgaata cgccgcactg gcagaactca aggctattgc
     2101 cagtcgcaat aaacgcttca cgtcttacat cggcatgggt tacaccgccg tgcagctacc
     2161 gccggttatc ctgcgtaaca tgctggaaaa tccgggctgg tataccgcgt acactccgta
     2221 tcagcctgaa gtctcccagg gccgccttga agcactgctc aacttccagc aggtaacgct
     2281 ggatttgact ggactggata tggcctctgc ttctcttctg gacgaggcca ccgctgccgc
     2341 cgaagcaatg gcgatggcga aacgcgtcag caaactgaaa aatgccaacc gcttcttcgt
     2401 ggcttccaat gtgcatccgc aaacgctgga tgtggtccgt actcgtgctg aaacctttgg
     2461 ttttgaagtg attgtcgatg acgcgcaaaa agtgctcgac catcaggacg tcttcggcgt
     2521 gctgttacag caggtaggca ctaccggtga aattcacgac tacactgcgc ttattagcga
     2581 actgaaatca cgcaaaattg tggtcagcgt tgccgccgat attatggcgc tggtgctgtt
     2641 aactgcgccg ggtaaacagg gcgcggatat tgtttttggt tcggcgcaac gcttcggcgt
     2701 gccgatgggc tacggtggcc cacacgcggc attctttgcg gcgaaagatg aatacaaacg
     2761 ctcaatgccg ggccgtatta tcggtgtatc gaaagatgca gctggcaata ccgcgctgcg
     2821 catggcgatg cagactcgcg agcaacatat tcgtcgtgag aaagcgaact ccaacatttg
     2881 tacttcccag gtactgctgg caaacatcgc cagcctgtat gccgtttatc acggcccggt
     2941 tggcctgaaa cgtatcgcta accgcattca ccgtctgacc gatattctgg cggcgggcct
     3001 gcaacaaaaa ggtctgaaac tgcgccatgc gcactatttc gacaccttgt gtgtggaagt
     3061 ggccgacaaa gcgggcgtac tgacgcgtgc cgaagcggct gaaatcaacc tgcgtagcga
     3121 tatcctgaac gccgtgggga tcacccttga tgaaaccacc acgcgtgaaa acgtaatgca
     3181 gcttttcagt gtgctgctgg gcgataacca cgggctggag atcgacacgc tggacaaaga
     3241 cgtggctcac gacagccgct ctatccagcc tgcgatgctg cgcgacgacg aaatcctcac
     3301 ccatccggtg tttaatcgct atcacagcga aaccgaaatg atgcgctata tgcactcgct
     3361 ggagcgtaaa gatctggcgc tgaatcaggc gatgatcccg ctgggttcct gcaccatgaa
     3421 actgaacgcc gccgccgaga tgatcccaat cacttggacg gaatttgccg aactgcaccc
     3481 gttttgcccg ccggaacagg ctgaaggtta tcagcagatg attgcgcagc tggctgactg
     3541 gctggtgaaa ctgaccggtt acgacgccgt ttgtatgcag ccgaactccg gcgcacaggg
     3601 cgaatacgcg ggcctgctgg cgattcgtca ttatcatgaa agccgcaacg aagggcatcg
     3661 cgatatctgc ctgatcccgg cttctgcgca cggaactaac cccgcttctg cacatatggc
     3721 aggaatgcag gtggtggttg tggcgtgtga taaaaacggc aacatcgatc tgactgatct
     3781 gcgcgcgaaa gcggaacagg cgggcgataa cctctcctgt atcatggtga cttatccttc
     3841 tacccacggc gtgtatgaag aaacgatccg tgaagtgtgt gaagtcgtgc atcagttcgg
     3901 cggtcaggtt taccttgatg gcgcgaacat gaacgcccag gttggcatca cctcgccggg
     3961 ctttattggc gcggacgttt cgcacctcaa cctgcataaa actttctgca ttccgcacgg
     4021 cggtggtggt ccgggtatgg gaccgatcgg tgtgaaagcg catctggcac cgtttgtacc
     4081 gggtcatagc gtggtgcaaa tcgaaggcat gttaacccgt cagggcgcag tttctgcggc
     4141 accgttcggt agcgcctcta tcctgccaat cagctggatg tacatccgca tgatgggcgc
     4201 agaagggctg aaaaaagcaa gccaggtggc aatcctcaac gccaactata ttgccagccg
     4261 cctgcaggat gccttcccgg tgctgtatac cggtcgcgac ggtcgcgtgg cgcacgaatg
     4321 tattctcgat attcgcccgc tgaaagaaga aaccggcatc agcgagctgg acattgccaa
     4381 gcgcctgatc gactacggtt tccacgcgcc gacgatgtcg ttcccggtgg cgggtacgct
     4441 gatggttgaa ccgactgaat ctgaaagcaa agtggaactg gatcgcttta tcgacgcgat
     4501 gctggctatc cgcgcagaaa ttgaccaggt gaaagccggt gtctggccgc tggaagataa
     4561 cccgctggtg aacgcgccgc acattcagag cgaactggtc gccgagtggg cgcatccgta
     4621 cagccgtgaa gttgcggtat tcccggcagg cgtggcagac aaatactggc cgacggtgaa
     4681 acgtctggat gatgtttacg gcgaccgtaa cctgttctgc tcctgcgtac cgattagcga
     4741 ataccagtaa ttcactgatt cgagtatttt ctaaaggcgc ttcggcgcct ttttagtcag
     4801 atgacaaagt acaaaagtgc tcagacagtc ccctcgcccc tttggggaga gggttagggt
     4861 gaggggaaca ggccggcact ggcgcgaata tttaccctca ccccgaccct ctccctgaaa
     4921 gggcgagggg gaaaagcgtg ccaacattga agattgagcc agtttgttag caatctcaaa
     4981 gatacgtcaa cgaattaatt tttctcggaa aaacaaatgg ctatagcact tgtgaccggt
     5041 ggcagtcgcg gcatcgggcg ggcaactgca ttactgttgg cgcaagaagg gtatacggtg
     5101 gcggttaatt atcagcaaaa cctccacgcg gcgcaggaag tgatgaactt aataatgcaa
     5161 gccggtggca aagcattcgt gctccaggcg gatatcagcg acgaaaacca ggtcgttgcg
     5221 atgtttacag caatcgatca gcacgatgaa ccgctagcag cgctggtcaa taacgccggg
     5281 atcttgttta cccagtgcac cgttgaaaac cttaccgcag agcgaatcaa ccgagtactt
     5341 tccaccaacg tgacgggata ttttctctgc tgccgcgagg cggtaaaacg catggcgctt
     5401 aaaaatggtg gcagtggcgg cgctatcgtc aatgtctctt cggtggcctc acggttgggt
     5461 tcgccagggg aatatgttga ttacgcggca tcgaaagggg cgattgattc gttaaccacc
     5521 ggactatcgc tggaagtcgc cgcgcagggg atccgcgtta actgcgtgcg gccagggttt
     5581 atttataccg aaatgcacgc cagcggcggc gagcctggac gcgtcgatcg cgttaagtcg
     5641 aacatcccca tgcagcgtgg tggacaggca gaagaggtcg cgcaggccat tgtctggcta
     5701 ctaagtgata aagcctctta cgtcacggga agttttatcg atttggcggg cgggaaataa
     5761 aacagggaag ttgtctgacc ggatgcaaca agtattgcat ccggtacttc atcgacttaa
     5821 agcttctcgc cgttgctggc aatcacctct ttgtaccagt taaagctctt cttacgtgaa
     5881 cgcgacatat cgccagtacc gtcgtcatgt ttattcacat agataaagcc gtagcgtttg
     5941 ctgtactgcc cggtagtgaa tgacacgcag tcgatgcagc cccacggtgt gtagcccatc
     6001 agatccacac catcgtaagt cactgctttt ttcatctctt caatatgggc gcgcaggtag
     6061 tcaatgcggt agtcgtcgtt gatgctgcca tcttcttcca ctttgtcgta agcgccaaaa
     6121 ccgttttcga caataaacag cggcctctga taacgctcat acagttcgca aagggcatag
     6181 cgcagaccta ctggatcaat ctgccagccc cagtcagacg ctttaacata cgggttcggt
     6241 acgctgcctt caaaaccaga gatcgcatcg ccggtgccgc cttcggcctt cactgcgttg
     6301 gtcatgtaat agctgaaacc aagataatcg caggtgcctt cgcgcagtac atcgagatcg
     6361 ccgtcttcca ttttgatatt aaatccgcga cgttcccact cgttcaatac ataggacggg
     6421 taatagccgc atagctgcac atcggtaaag acgtagcgtt cgcgcatcga ctcctgagcg
     6481 aacatcacat cgtccgggtt acatgagtag ggatagagcg gcaccatcgc cagcatacag
     6541 ccgactttca tctccgggtt aatgcgacgc gcggctttca ctgccagggc gctggcgaca
     6601 aactggtgat gcagcacctg gtacatcgtc tcttccgggt tgtcatgctc ggtatacacc
     6661 acgccggagc agcagtaacc aaacagcggc gcacgccagt tacgctggtt gtttatttcg
     6721 ttgaaggtca tccagtattt gactttatgc ttgtagcgtt cgaataccac ttccgcgaaa
     6781 cggacaaaga aatcaacgaa tttacggttg gtccagctac cgtattgctg caccagatgc
     6841 agcggcattt caaagtggga gagggttatc accggttcga tgttgtattt cagcagttca
     6901 tcgaacagag agtcgtaaaa cttcagccct tcttcgtttg gctgagcttc atcacctttt
     6961 ggaaaaatac gcgtccaggc gatggaagtc cgaaaacatt tgaagcccat ttcggcaaat
     7021 agcttgatgt cttccttgta gtgaccataa aaatcaacgg cttcatggtt tggatagtat
     7081 tttcctggca agacttcttt ggtgatttcg cgcggcacgc cgtgtgcgcc acaggtcaga
     7141 acgtcacaaa tgctcggtcc tttgccgcct ttgttccagc cgccttcgac ctgatgagcg
     7201 gcaacggcgc caccccataa gaaatctttc ggtaaggtga gttttttcac tatcataggc
     7261 tccagatcag tcaattctta caggaaatag taccatgcag ccaaacgaca tcactttttt
     7321 caacgtttcc aggatgacat tctggctggg cgtaaaacca tcaccatccg cgacgagtct
     7381 gaatcgcact tcaaaacggg tgatgtgctt cgtgtcggac gttttgaaga tgacggttat
     7441 ttttgcacga ttgaagtcac cgcaacctca accgtaacgc tggatacact gacggaaaaa
     7501 catgcagagc aggaaaatat gaccctgcct gaactgaaaa aggtcattgc tgacatctat
     7561 cccggtcaga cacaatttta tatgattgaa tttaaatgtc tttaaattga actcacgcct
     7621 gttagttgaa atttcgcatt taatttcatg atttttagga gattagtctc aaatcagaat
     7681 ttattttagc taacaggtgt tcactggaac tattctcagt tacgctggag aggtatacat
     7741 gtgcgtgtac gtttctctgg agtaagttat ggttcagaag cccctcatta agcagggata
     7801 ttcgctggca gaggaaattg ccaacagcgt cagtcacggc attgggctgg tgtttggtat
     7861 cgttgggctg gtgttgctac tggttcaggc ggtggatctc aatgccagcg ccacagcgat
     7921 aaccagttac agcctctatg gcggcagtat gatcctgctg ttcctcgctt cgacgctcta
     7981 tcacgccatt cctcatcaac gggcaaaaat gtggctgaag aaatttgacc attgcgctat
     8041 ttacctgttg attgccggaa cctacacgcc gtttttgctg gtggggctgg attctccgtt
     8101 agcgcgcggg ttgatgattg ttatctggag cctagcattg ctggggattc tgttcaaact
     8161 gaccatcgcg caccgattca aaattttatc tctggtgact tatctggcga tgggctggct
     8221 gtcgctggtg gtaatttatg aaatggcagt taagctcgcg gcgggcagcg ttaccttact
     8281 ggcggtaggc ggcgtggttt attcgctcgg ggtgattttc tacgtctgca aacgcattcc
     8341 atacaaccat gccatctggc acggcttcgt gctcggcggt agtgtgtgcc actttctggc
     8401 gatctatttg tatattgggc aggcgtaatg ggtatgcctt tgtaggcctg ataagacgcg
     8461 tcagcgtcgc atcaggcatc ggagcgcaac cgccggatgc ggcgtgaacg ccttatccga
     8521 tctacgggcc tcgtccctgt tgtgataaag gcatcaaatc ccagacatga taagacgcgt
     8581 cagcgtcgca tcaggcgata aatgcctgat gcgatacaga gatttactct tcgagcgaat
     8641 acggcagcgg ctcgatatgc agtgtattcg catcgtcgcg tacgcggaag atgctgtccg
     8701 gttccatatc gttattcatg acgacctgta ccacgacctg accatcttcc agttttaccg
     8761 cagccagcac cgtaccggta cgacgccagt tctcgcccat tttcagctct aagtcttcac
     8821 cagcttccgg cagtcggctg gcactacctg ccagcaacca gagcgcgcgt ttgttggcac
     8881 cacggaattt tgctcgcgcc accatctctt gtccggtata acagcctttc ttaaagctga
     8941 taccgcccag cgcctggaga ttggtcgcct gtgggataaa ctgcccgctg ttggcggcat
     9001 caatcaccgg gaaaccagct tcaatgttta atgccagcca ctgttggcta ttgttcagtt
     9061 ccgcttcacc gcgcagttta tcggtcagca tattagcagt agcttcatcg gttacgatca
     9121 ggaaacgttc tgccgggtgt tcaaaccata gcaaagtggt cgcgccttct ttgactacct
     9181 gtttttcttt cgaaggcagt tcgctaaaga gatttgccag cgcggcgcgc gcctgaaaac
     9241 cggcaacacc aagcagcaca cgctcgtcgt ctggcgcgat ggtcacttta gagaataccg
     9301 catatttttt cagttcagtc agctgcggtt cacgcacgct gcgccgttca atccatgcaa
     9361 agccatcgcc gtcgcggaac agacgtaaat tgctccacat tttacctttg gcgtcgcaat
     9421 gggcggcgag caggtgctga tcttctgcca tctggctgac atctgctgtc acctgaccct
     9481 gcatatattt ttcgctgtcc gcgccagtaa tggtggcaag cgcccagtca tcaagcgtca
     9541 tcagcgtcag cggtaaacgg gcagaagccg taggctgacg tggaggaaaa ggtgtaaaag
     9601 ccataataga gtcctgagtt gcttaacggc gtattaatga ctaatggtaa aagagctatg
     9661 aggcaatgca agtgctttca ctacgcgatc tgtgccttct gcgaggcgac tgccaaaaaa
     9721 atgaagccct tgaattgttg tgaattaatt tcgcgatcgc gatggtgttt actgatattc
     9781 ccgtaaaaga agtcgaaaaa cacgttacaa tagccgggta ctggattttc gtgagacagg
     9841 aagaagatgg acattaacaa caaagcccga attcattggg catgccgccg tggtatgcgc
     9901 gaactcgata tttcaatcat gtcgtttttc gaacatgagt acgacagctt aagcgatgac
     9961 gaaaaacgca tctttattcg tctgctggaa tgtgacgatc cggacctgtt taactggctg
    10021 atgaatcacg gtaaaccggc cgatgcagaa ctggaaataa tggtccgact catccagaca
    10081 cggaaccggg aacgtggtcc tgtggcaatc tgatttgcgc gtctcctggc gcgcacagtg
    10141 gctttccttg ctgattcatg ggctggttgc cgctgttatt ttactcatgc cctggccgct
    10201 cagttacacc ccgttatgga tggtgttact ttcgctggtg gtatttgatt gcgttcgcag
    10261 ccagcggtgt attaatgctc gccaggggga aattcgcttg ttgatggacg ggcgtttgcg
    10321 ttggcaaggg caggagtgga gcatcgtcaa agcaccgtgg atgattaaga gcggcatgat
    10381 gctgcgttta cgttctgatg gcggtaaacg gcaacattta tggctggcag ccgacagcat
    10441 ggacgaagcc gaatggcggg atttacggcg gattttgttg caacaagaga cgcaaagata
    10501 aagatgacgg caaagcatat gcgctaagcc gtcagtgaga tcaggcgtaa tgctctgcca
    10561 tttcgttgag gatttgctcg caccagctct gaatacgctc gtcgctaagg tcatactggt
    10621 tagtttcatc cagcgccaga cccacgaaca gttgcccgtc agcaatcacc ggtttcgggc
    10681 tggtaaattc atatccttcc gttggccagt agccgacgaa cttcacgcct ttggtcgaga
    10741 gtttgtcatg cagcataccg agcgcatcga ggaaccactc gccgtatccc agttgatcgc
    10801 caagcccata cagcgcaata attttacctt caaggttcag gtcgtcgagc tgatcccaga
    10861 cggcttccca gtcttcctgg atttcaccaa aatcccaggt cgggataccc agaatgagca
    10921 catcgtactg ctccattaat ttcggggagt cgtccttgag gttatgtaag gtcaccagtt
    10981 ctgggccgat aatatcgcgg attttttctg ccgccatttc ggtgtaacag gtgctggaac
    11041 cgtaaaaaag acccatattc ataacgtaag cttctcgatt ctgctgtggc ttttgcagct
    11101 agtgtatcag aatcgcttta gtcacggcac aataaggcat aatggatcaa atgagtgacc
    11161 ataaggggcg caagtgaaac aggagctggc acgcatcgag cagtttcttg atgctctgtg
    11221 gctggaaaaa aatctggctg aaaatacgtt gaacgcttac cgtcgcgatc tgtcaatgat
    11281 ggtggagtgg ttgcatcacc gcgggttgac gctggcgacg gcgcaaagtg acgatttgca
    11341 ggcattactg gcagaacggc tggagggcgg gtataaagcc accagctcag cgcgtttgct
    11401 gagtgcagtg cgccgattgt tccagtatct ttatcgcgaa aagtttcgtg aagacgatcc
    11461 cagtgcgcat ctcgcttcac cgaaattgcc ccagcgtttg ccaaaagatt taagcgaagc
    11521 gcaggtcgaa cgtttattac aggcgccatt aattgatcag ccactggagc tacgcgataa
    11581 agccatgctt gaagtgttgt atgctaccgg gctgcgtgtc tctgaactgg tcgggctgac
    11641 aatgagtgat atcagcctgc gtcagggcgt agtgcgggtc attggtaaag gcaacaaaga
    11701 gcgcctggtg ccgttagggg aagaggcggt ttactggctg gaaacctatc tggaacatgg
    11761 gcgtccgtgg ctgctgaatg gtgtgtcaat tgacgtgttg tttcccagcc agcgtgcgca
    11821 gcagatgacg cgacagacct tctggcaccg cattaaacat tatgcagtgc tggcgggtat
    11881 cgacagcgaa aagctgtcac cgcatgtgtt gcgtcacgct tttgccactc atttattaaa
    11941 tcatggtgcg gatttacgcg tggtgcagat gctactgggc cacagcgatc tttccaccac
    12001 gcaaatttat acgcatgtcg ctaccgagcg tctgcggcaa cttcatcaac agcatcaccc
    12061 gcgggcgtga tgtctgaaaa gaacgggaag atttatgaag aaaggtttta tgttgtttac
    12121 tttgttagcg gcgttttcag gctttgctca ggctgatgac gcggcaattc aacaaacgtt
    12181 agccaaaatg ggcatcaaaa gcagcgatat tcagcccgcg cccgtagctg gcatgaagac
    12241 agttctgact aacagcggtg tgttgtacat caccgatgat ggtaaacata tcattcaggg
    12301 gccaatgtat gacgttagtg gcacggctcc ggtcaatgtc accaataaga tgctgttaaa
    12361 gcagttgaat gcgcttgaaa aagagatgat cgtttataaa gcgccgcagg aaaaacacgt
    12421 catcaccgtg tttactgata ttacctgtgg ttactgccac aaactgcatg agcaaatggc
    12481 agactataac gcgctgggga tcaccgtgcg ttatcttgct ttcccgcgcc aggggctgga
    12541 cagcgatgca gagaaagaaa tgaaagctat ctggtgtgcg aaagataaaa acaaagcgtt
    12601 tgatgatgtg atggcaggta aaagcgtcgc accagccagc tgcgacgtgg atattgccga
    12661 ccattacgca cttggcgtcc agcttggcgt tagcggtact ccggcagttg tgctgagcaa
    12721 tggcacactt gttccgggtt accagccgcc gaaagacatg aaagaattcc tcgacgaaca
    12781 ccaaaaaatg accagcggta aataattcgc gtgaaacaac agatacaact tcgtcgccgt
    12841 gaagtcgatg aaacggcaga cttgcccgct gaattgcctc ccttgctgcg ccgtttatac
    12901 gccagccggg gagtacgcag tgcgcaagaa ctggaacgca gtgttaaagg tatgttgccc
    12961 tggcagcaac taagcggtgt cgaaaaggcc gttgagatcc tttacaacgc ttttcgcgaa
    13021 ggaacgcgga ttattgtggt cggtgatttc gacgccgacg gcgcgaccag cacggctcta
    13081 agcgtgctgg cgatgcgctc gcttggttgc agcaatatcg actacctggt accaaaccgt
    13141 ttcgaagacg gttacggctt aagcccggaa gtggtcgatc aggcccatgc ccgtggcgcg
    13201 cagttaattg tcacggtgga taacggtatt tcctcccatg cgggagttga gcacgctcgc
    13261 tcgttgggca tcccggttat tgttaccgat caccatttgc cgggcgacac attacccgca
    13321 gcggaagcga tcattaaccc taacttgcgc gactgtaatt tcccgtcgaa atcactggca
    13381 ggcgtgggtg tggcgtttta tctgatgctg gcgctgcgca cctttttgcg cgatcagggc
    13441 tggtttgatg agcgcggcat cgcaattcct aacctggcag aactgctgga tctggtagca
    13501 ctggggacag tagcggacgt cgtgccgctg gacgctaata atcgcattct gacctggcag
    13561 gggatgagtc gcatccgtgc cggaaagtgc cgtccaggga ttaaagcgct gctggaagtg
    13621 gcaaatcgcg atgcacaaaa actagccgcc agcgatttag gttttgcgct gggaccgcgt
    13681 ctcaatgctg ccgggcgact ggatgatatg tccgttggtg tggcgctgtt gctgtgcgac
    13741 aacatcggcg aagcgcgcgt gctggcaaat gaactcgatg cgctaaacca gacgcgaaaa
    13801 gagatcgaac aaggaatgca ggttgaagcc ctgaccctgt gcgagaaact ggagcgcagc
    13861 cgtgacacgc tacccggcgg gctggcaatg tatcaccccg aatggcatca gggcgttgtc
    13921 ggtattctgg cttcgcgtat caaagagcgt tttcaccgtc cggttatcgc ctttgcgcca
    13981 gcaggtgacg gtacgctgaa aggttccggt cgctccattc aggggctgca tatgcgtgat
    14041 gcgctggagc gattagacac actctacccc ggcatgatgc tcaagtttgg cggtcatgcg
    14101 atggcggcgg gtttgtcgct ggaagaggat aaattcgaac tctttcaaca acggtttggc
    14161 gaactggtta ctgagtggct ggccccttcg ctattgcaag gcgaagtggt gtcagacggt
    14221 ccgttaagcc cggccgaaat gaccatggaa gtggcgcagc tgctgcgcga tgctggcccg
    14281 tgggggcaga tgttcccgga gccgctgttt gacggtcatt tccgtctgct gcaacagcgg
    14341 ctggtgggcg aacgtcattt gaaggtgatg gtcgaaccgg tcggcggcgg tccactgctg
    14401 gatggtattg cttttaatgt cgataccgct ctctggccgg ataacggcgt gcgcgaagtg
    14461 caactggctt ataagctcga tatcaacgag tttcgcggca accgcagcct gcaaattatc
    14521 atcgacaata tctggccaat ttagcgtcat cttctctata aaaaagagcg tggattgggt
    14581 acaatcccgc tcttatcacc gcattttgac tagctcaata aaagaaatca gaccatgttt
    14641 gaaattaatc cggtaaataa tcgcattcag gacctcacgg aacgctccga cgttcttagg
    14701 gggtatcttt gactacgacg ccaagaaaga gcgtctggaa gaagtaaacg ccgagctgga
    14761 acagccggat gtctggaacg aacccgaacg cgcacaggcg ctgggtaaag agcgttcctc
    14821 cctcgaagcc gttgtcgaca ccctcgacca aatgaaacag gggctggaag atgtttctgg
    14881 tctgctggaa ctggctgtag aagctgacga cgaagaaacc tttaacgaag ccgttgctga
    14941 actcgacgcc ctggaagaaa aactggcgca gcttgagttc cgccgtatgt tctctggcga
    15001 atatgacagc gccgactgtt acctcgatat tcaggcgggg tctggcggta cggaagcaca
    15061 ggactgggcg agcatgcttg agcgtatgta tctgcgctgg gcagaatcgc gtggtttcaa
    15121 aactgaaatc atcgaagagt cggaaggtga agtggcgggt attaaatccg tgacgatcaa
    15181 aatctccggc gattacgctt acggctggct gcgtacagaa actggcgttc accgcctggt
    15241 gcgtaaaagc ccgtttgact ccggcggtcg tcgccacacg tcgttcagct ctgcgtttgt
    15301 ttatccggaa gttgatgatg atattgatat cgaaatcaac ccggcggatc tgcgcattga
    15361 cgtttatcgc gcgtccggcg cgggcggtca gcacgttaac cgtaccgaat ctgcggtgcg
    15421 tattacccac atcccgaccg ggatcgtgac ccagtgccag aacgaccgtt cccagcacaa
    15481 gaacaaagat caggccatga agcagatgaa agcgaagctt tatgaactgg agatgcagaa
    15541 gaaaaatgcc gagaaacagg cgatggaaga taacaaatct gacatcggct ggggcagcca
    15601 gattcgttct tatgtccttg atgactcccg cattaaagat ctgcgcaccg gggtagaaac
    15661 ccgcaacacg caggccgtgc tggacggcag cctggatcaa tttatcgaag caagtttgaa
    15721 agcagggtta tgaggaacca acatgtctga acaacacgca cagggcgctg acgcggtagt
    15781 cgatcttaac aatgaactga aaacgcgtcg tgagaagctg gcgaacctgc gtgagcaggg
    15841 aattgccttc ccgaacgatt tccgtcgcga tcatacctct gaccaattgc acgcagaatt
    15901 cgacggtaaa gagaacgaag aactggaagc gctgaacatc gaagtcgccg ttgctggccg
    15961 catgatgacc cgccgtatta tgggtaaagc gtctttcgtt accctgcagg atgtgggtgg
    16021 ccgcattcag ctgtacgttg cccgtgacga tctgccggaa ggcgtttaca acgagcagtt
    16081 caaaaaatgg gacctcggcg acatcctcgg cgcgaaaggt aaactgttca aaaccaaaac
    16141 cggcgaactg tctatccact gtaccgagct gcgtctgctg accaaagcac tgcgtccgct
    16201 gccggataaa ttccacggct tgcaggatca ggaagcgcgc tatcgtcagc gttatctgga
    16261 tctcatctct aacgatgaat cccgtaacac ctttaaagta cgctcgcaga tcctctctgg
    16321 tattcgccag ttcatggtga accgcggctt tatggaagtt gaaacgccga tgatgcaggt
    16381 gatcccaggc ggtgccgctg cgcgtccgtt tatcacccac cataacgcgc tggatctcga
    16441 catgtacctg cgtatcgcgc cggaactgta cctcaagcgt ctggtggtcg gtggcttcga
    16501 gcgtgtattc gaaatcaacc gtaacttccg taacgaaggt atttccgtac gtcataaccc
    16561 agagttcacc atgatggaac tctacatggc ttacgcagat tacaaagatc tgatcgagct
    16621 gaccgaatcg ctgttccgta ctctggcaca ggatattctc ggtaagacgg aagtgaccta
    16681 cggcgacgtg acgctggact tcggtaaacc gttcgaaaaa ctgaccatgc gtgaagcgat
    16741 caagaaatat cgcccggaaa ccgacatggc ggatctggat aacttcgact ctgcgaaagc
    16801 gattgctgaa tctatcggta tccacgttga gaagagctgg ggtctgggcc gtatcgttac
    16861 cgagatcttc gaagaagtgg cagaagcaca tctgattcag ccgaccttca ttactgaata
    16921 tccggcagaa gtttctccgc tggcgcgtcg taacgacatt aacccggaaa tcacagaccg
    16981 ctttgagttc ttcatcggtg gtcgtgaaat cggtaacggc tttagcgagc tgaatgacgc
    17041 ggaagatcag gcgcaacgct tcctggatca ggttgccgcg aaagacgcag gtgacgacga
    17101 agcgatgttc tacgatgaag attacgttac cgcactggaa catggcttac cgccgacagc
    17161 aggtctgggg attggcatcg accgtatggt aatgctgttc accaacagcc ataccatccg
    17221 cgacgttatt ctgttcccgg cgatgcgtcc ggtgaaataa gcattacgtt atgctcacaa
    17281 ccccggcaaa tgtcggggtt ttttatttaa gctgggtaaa tgcagataat cgtattctgg
    17341 cttcgcgatt tgtcgcctgc atcaccatcc acggactgaa cgcccacggc gtggcatcaa
    17401 taccgcgtaa tacatcagct aaatcacacc attgataatc catcacttca tcatcgttga
    17461 tctgtaacgc actggtggtg cgtgcggcaa ataccggaca cacttcattt tccacaatgc
    17521 ctctcggatc ggtggcgcgg tagcgaaagt caggatagat agattcagga ggcgtaattt
    17581 ccacgccaag ctcataacgg caacggcgga tcaccgcgtc ttcgctgctt tctcccagtt
    17641 gtgggtgccc acaaaccgag ttagtccaca cgccaggcca cgcttttttg ctcagtgcgc
    17701 ggcgggtaac taataattgc cccttggcat taaacagcca actggagaac gcgagatgta
    17761 agagggtgtc tgccgtgtgt gcggcatact tttccagcgt acccgtggga actccctgtg
    17821 cattcaataa aatgacgtgt tccgtttgca taatttctca catgtaattc tgatcactca
    17881 tcattataga taattgaaga gtgaaatgat taaccaagag agcgtttatc gacaaccgcc
    17941 cgttttgagc ggatgccgac aggtaaaaac catttaaaaa agtagcatca tatatttgaa
    18001 gcgactttca atggccattg caacacagtc agaaatgaca ggaatagaga gagtaaaaag
    18061 caacaacaga ctgaatatag cgcgcattaa aaaattcata ttattctcca tgctcatttt
    18121 tcttcagctt gcgcatcaga agccacatac gtactgtcct gacttctaaa tctttatcag
    18181 agacggattc cttcaccagg tcagcatgat gctgatagcc attaaagaag acgttcagca
    18241 caacggcgct aagggttgcg agcataatgc cgctatgtag cagcggttgc agtacggccg
    18301 gtaacttaga aaagaaatcg tgagagagcg tcggagtcat gccaacgccg agactgatcg
    18361 ccacaatata gaggttgtaa cggttggtgg tgtagttaca gcgcgacaga attcgaatcc
    18421 ctgtcgccag taccatgccg aacatcacta gaccagcgcc gcccagcaca aattgcggaa
    18481 tggaggctac cagcaccgcc atttttggca ccatgccaaa caggattaat ataattcccg
    18541 aagaaataca cacccagcgg ctatgaacgc gcatcacgct aaccaggcca acgttttgag
    18601 aaaatgacgt gtgggggaag ctgttaaacg tgccgcctat cattgtccct acgccatcga
    18661 cacgcagccc gcaaataata tcgtgtgaag agagtttacg accgactatt tcacccagtg
    18721 ccaggaacat ccccatcgac tcgataaaca cgatgattaa cacggcagtc atggtcagaa
    18781 tggaaacggg atcgaaaatc ggcataccaa acgacatcgg ggtgacaatc gcaaaccacg
    18841 aggcatcatg tagcccggat aaattgactt cattcatcat ccacgaaagt aaaaagccaa
    18901 atacaatccc cagtaatacg gcgacgttgg acataaatcc tttcgcatag cgagtaatga
    18961 gcaagataaa aattaagacg gcaaaggaga tacctaaata aacgggatta ccatattgcg
    19021 gattcccttt acctccagcg gcccagtcaa tacccacctg aatgatgcta agcccaatag
    19081 aagtaataac cacaccggta accagtggcg ggaataaagg catcaagcga ccaataagtg
    19141 gcgctaataa tgtggtgata aaacccgcgg cgatagtggc accaaatatc cccagcaggc
    19201 cgatatccgg gttcatccca atggctatca ttggtgttac agcagcaaag gtcaccgaca
    19261 taatcaccgg caggcggatc cccataaagc ggccgatacc gatacattgc aataatgtga
    19321 cgatcccgca gcaaaagaga tccgagctaa tgagcatcgc aatagcatct tttgagaggc
    19381 ccagtcggtc accaatcata agaggaacag cgactgcacc tgcgtacatg acaaggacgt
    19441 gttgcagacc gagtatgatc agctttcctg gtgataatat gcggtcaacc tcatcagttg
    19501 ggcggtcttg cccagaagat gagggaagtt gggaatctat ggcgctcatc agtgtttctc
    19561 ctttcgcccg ccagaataaa aagcaaccgt ttacgttaat gaaactgtct aaacacatgc
    19621 tcgtggagtg ccagtttttg cgaggaaggg gagttagaga caggaattgc ggaggcaatc
    19681 acataatcgt gcttaaacca cgaaaatgac aagagaaact gacaatggaa ttatctgtaa
    19741 ttttctcata ttgatatttc attaatcatt ctggcatgtc gcactctcgc atttaatcgt
    19801 ttttatctgg atagcgctct tttgatcggc aatgttgaaa taatgaaagc ggcatagatt
    19861 gaggggcaat aatttgaact cagtcgcaaa attataatta aaacggtcat gctacctaat
    19921 atatgcccgt ttattgcatc gcgaaatttg agaagacata actcattaaa tgaaagggat
    19981 gcaaatgaat acgtttatcg ctgctgaagc tgcggaatgt ataggctgtc
//
LOCUS       DAZPCS010000063        19820 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZPCS010000063 DAZPCS010000000
VERSION     DAZPCS010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19820
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.063"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            384..805
                     /gene="atpI"
                     /locus_tag="ACHUQ7_002184"
                     /pseudo
     CDS             384..805
                     /gene="atpI"
                     /locus_tag="ACHUQ7_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
     gene            814..1629
                     /gene="atpB"
                     /locus_tag="ACHUQ7_002185"
     CDS             814..1629
                     /gene="atpB"
                     /locus_tag="ACHUQ7_002185"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFR1135586.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            1676..1915
                     /gene="atpE"
                     /locus_tag="ACHUQ7_002186"
     CDS             1676..1915
                     /gene="atpE"
                     /locus_tag="ACHUQ7_002186"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFR1135587.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            1977..2447
                     /gene="atpF"
                     /locus_tag="ACHUQ7_002187"
     CDS             1977..2447
                     /gene="atpF"
                     /locus_tag="ACHUQ7_002187"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFR1135588.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            2462..2995
                     /gene="atpH"
                     /locus_tag="ACHUQ7_002188"
     CDS             2462..2995
                     /gene="atpH"
                     /locus_tag="ACHUQ7_002188"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFR1135589.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLTFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            3008..4549
                     /gene="atpA"
                     /locus_tag="ACHUQ7_002189"
     CDS             3008..4549
                     /gene="atpA"
                     /locus_tag="ACHUQ7_002189"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFR1135590.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            4600..5463
                     /gene="atpG"
                     /locus_tag="ACHUQ7_002190"
     CDS             4600..5463
                     /gene="atpG"
                     /locus_tag="ACHUQ7_002190"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFR1135591.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            5490..6872
                     /gene="atpD"
                     /locus_tag="ACHUQ7_002191"
     CDS             5490..6872
                     /gene="atpD"
                     /locus_tag="ACHUQ7_002191"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFR1135592.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            6893..7312
                     /locus_tag="ACHUQ7_002192"
     CDS             6893..7312
                     /locus_tag="ACHUQ7_002192"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFR1135593.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            7663..9033
                     /gene="glmU"
                     /locus_tag="ACHUQ7_002193"
     CDS             7663..9033
                     /gene="glmU"
                     /locus_tag="ACHUQ7_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFR1135594.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            9195..11024
                     /gene="glmS"
                     /locus_tag="ACHUQ7_002194"
     CDS             9195..11024
                     /gene="glmS"
                     /locus_tag="ACHUQ7_002194"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFR1135595.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            11186..12007
                     /locus_tag="ACHUQ7_002195"
     CDS             11186..12007
                     /locus_tag="ACHUQ7_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsA endonuclease N-terminal domain-containing
                     protein"
                     /protein_id="HFR1135596.1"
                     /translation="MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRS
                     HRIYSHKTGRVHHLLSDLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHP
                     VIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPW
                     FIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVD
                     IAYDLELGKTLSEIRALTANGFIKFNIYKSFRANKCADLCISQVVNMEELRYVAN"
     gene            11994..14102
                     /locus_tag="ACHUQ7_002196"
     CDS             11994..14102
                     /locus_tag="ACHUQ7_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFR1135597.1"
                     /translation="MWQINEVVLFDNDPYRILAIEDGQVVWMQISADKGVPQARAELL
                     LMQYLDEGRLVRTDDPYVHLDLEEPSVDSVSFQKREEDYRKILPIINSKDRFDPKVRS
                     ELVEHVVQEHKVTKATVYKLLRRYWQRGQTPNALIPDYKNSGAPGERRSATGTAKIGR
                     AREYGKGEGTKVTPEIERLFRLTIEKHLLNQKGTKTTVAYRRFVDLFAQYFPRIPQED
                     YPTLRQFRYFYDREYPKAQRLKSRVKAGVYKKDVRPLSSTATSQALGPGSRYEIDATI
                     ADIYLVDHHDRQKIIGRPTLYIVIDVFSRMITGFYIGFENPSYVVAMQAFVNACSDKT
                     AICAQHDIEISSSDWPCVGLPDVLLADRGELMSHQVEALVSSFNVRVESAPPRRGDAK
                     GIVESTFRTLQAEFKSFAPGIVEGSRIKSHGETDYRLDASLSVFEFTQIILRTILFRN
                     NHLVMDKYDRDADFPTDLPSIPVQLWQWGMQHRTGSLRAVEQEQLRVALLPRRKVSIS
                     SFGVNLWGLYYSGSEILREGWLQRSTDIARPQHLEAAYDPVLVDTIYLFPQVGSRVFW
                     RCNLTERSRQFKGLSFWEVWDIQAQEKHNKANAKQDELTKRRELEAFIQQTIQKANKL
                     TPSTTEPKSTRIKQIKTNKKEAVTSERKKRAEHLKPSSSGDEAKVIPFNAVEADDQED
                     YSLPTYVPELFQDPPEKDES"
     gene            14099..15766
                     /locus_tag="ACHUQ7_002197"
     CDS             14099..15766
                     /locus_tag="ACHUQ7_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR1135598.1"
                     /translation="MSATRIQAVYRDTGVEAYRDNPFIEALPPLQESVNSAASLKSSL
                     QLTSSDLQKSRVIRAHTICRIPDDYFQPLGTHLLLSERISVMIRGGYVGRNPKTGDLQ
                     KHLQNGYERVQTGELETFRFEEARSTAQSLLLIGCSGSGKTTSLHRILATYPQVIYHR
                     ELNVEQVVYLKIDCSHNGSLKEICLNFFRALDRALGSNYERRYGLKRHGIETMLALMS
                     QIANAHALGLLVIDEIQHLSRSRSGGSQEMLNFFVTMVNIIGVPVMLIGTPKAREIFE
                     ADLRSARRGAGFGAIFWDPIQQTQRGKPNQEWIAFTDNLWQLQLLQRKDALLSDEVRD
                     VWYELSQGVMDIVVKLFVLAQLRALALGNERITAGLLRQVYQDELKPVHPMLEALRSG
                     IPERIARYSDLVVPEIDKRLIQLQLDIAAIQEQTPEEKALQELDTEDQRHLYLMLKED
                     YDSSLLIPTIKKAFSQNPTMTRQKLLPLVLQWLMEGETVVSELEKPSKSKKVSAIKVV
                     KPSDWDSLPDTDLRYIYSQRQPEKTMHERLKGKGVIVDMASLFKQAG"
     gene            15769..17295
                     /locus_tag="ACHUQ7_002198"
     CDS             15769..17295
                     /locus_tag="ACHUQ7_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsD family Tn7-like transposition protein"
                     /protein_id="HFR1135599.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVA
                     TLGLPSHLGVIARHLHQTGRYAVQQLIYEHTLFPLYAPFVGKERRDEAIRLMEYQAQG
                     AVHLMLGVAASRVKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVF
                     FDRAVDDHRHQFWALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQW
                     TLFYQRLAQDLGLTKSKHIRHDLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFR
                     KHRKAFSYLQHSIVWQALLPKLTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVK
                     HKDWQQLVHKYQGIKAARQSLEGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDW
                     NQRDRIAVRQLLRIIKRLDSSLDHPRATSSWLLKQTPNGTSLAKNLQKLPLVALCLKR
                     YSESVEDYQIRRISQAFIKLKQEDVELRRWRLLRSATLSKERITEEAQRFLEMVYGEE
                     "
     gene            17296..18912
                     /locus_tag="ACHUQ7_002199"
     CDS             17296..18912
                     /locus_tag="ACHUQ7_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn7-like element transposition protein TnsE"
                     /protein_id="HFR1135600.1"
                     /translation="MVRLATFNDNVQVVHIGHLFRNSGHKEWRIFVWFNPMQERKWTR
                     FTHLPLLSRAKVVNSTTKQINKADRVIEFEASDLQRAKIIDFPNLSSFASVRNKDGAQ
                     SSFIYEAETPYSKTRYHIPQLELARSLFLINSYFCRSCLSSTALQQEFDVQYEVERDH
                     LEIRILPSSSFPKGALELSAVVQLLVWLFSDQDVMDSYESIFRHYQQNREIKNGVESW
                     CFSFDPPPMQGWKLHVKGRSSNEDKDYLVEEIVGLEINAMLPSTTAISHASFQEKEAG
                     DGSTQHIAVSTESVVDDEHLQLDDEETANIDTDTRVIEAEPTWISFSRPSRIEKSRRA
                     RKSSQTILEKEEATTSENSNLVSTDEPHLGGVLAAADVGGKQDATNYNSIFANRFAAF
                     DELLSILKTKFACRVLFEETLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLE
                     VDASDGVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGV
                     NHPKGLGELQVSREDMEGWAERVVREQFTH"
     gene            complement(18968..19120)
                     /locus_tag="ACHUQ7_002200"
                     /pseudo
     CDS             complement(18968..19120)
                     /locus_tag="ACHUQ7_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(19143..19520)
                     /locus_tag="ACHUQ7_002201"
     CDS             complement(19143..19520)
                     /locus_tag="ACHUQ7_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1135601.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
BASE COUNT         5175 a         4423 c         5162 g         5060 t
ORIGIN      
        1 tggtttttgt tgtgtaaatg ttatttatta aaacagtatc tgtttttaga ctgaaatatc
       61 ataaacttgt aaaggcatca tttgccaagt aaataaatat gctgtgcgcg aacatgcgca
      121 atatgtgatc tgaagcacgc tttatcacca gtgtttacgc gttatttaca gtttttcatg
      181 atcgaacagg ggtagcggaa aagtcgcaat tgtatgcact ggaaaaatat ttaaacattt
      241 attcaccttt tggctactta ttgtttgaaa tcacgggggc gcaccgtata atttgaccgc
      301 tttttgatgc ttgactctaa gccttaaaga aagttttata cgacacgcgg catacctcga
      361 agggagcagg agtgaaaaac gtgatgtctg tgtcgctcgt gagtcgaaac gttgctcgga
      421 agcttctgct cgttcagtta ctggtggtga tagcaagtgg attgctgttc agcctcaaag
      481 accccttctg gggcgtctct gcaataagcg ggggcctggc agtctttctg cctaacgttt
      541 tgtttatgat atttgcctgg cagtctttct gcctaacgtt ttgtttatga tatttgcctg
      601 gcgtcaccag gcgcatacac cagcgaaagg ccgggtggct tggacattcg catttggcga
      661 agctttcaaa gttctggcga tgttggtgtt actggtggtg gcgttggcgg ttttaaaggc
      721 ggtattcttg ccgctgatcg ttacgtgggt tttggtgctg gtggttcaga tactggcacc
      781 ggctgtaatt aacaacaaag ggtaaaaggc atcatggctt cagaaaatat gacgccgcag
      841 gattacatag gacaccacct gaataacctt cagctggacc tgcgtacatt ctcgctggtg
      901 gatccacaaa accccccagc caccttctgg acaatcaata ttgactccat gttcttctcg
      961 gtggtgctgg gtctgttgtt cctggtttta ttccgtagcg tagccaaaaa ggcgaccagc
     1021 ggtgtgccag gtaagtttca gaccgcgatt gagctggtga tcggctttgt taatggtagc
     1081 gtgaaagaca tgtaccatgg caaaagcaag ctgattgctc cgctggccct gacgatcttc
     1141 gtctgggtat tcctgatgaa tctgatggat ttactgccta tcgacttgct gccgtacatt
     1201 gctgaacatg tactgggtct gcctgcactg cgtgtggttc cgtctgcgga cgtgaacgta
     1261 acgctgtcta tggcactggg cgtatttatc ctgattctgt tctacaacat caaaatgaaa
     1321 ggcatcggcg gcttcacgaa agagttgacg ctgcagccgt tcaatcactg ggcgttcatt
     1381 cctgtcaact taatccttga aggggtaagc ctgctgtcca aaccagtttc actcggtttg
     1441 cgactgttcg gtaacatgta tgccggtgag ctgattttca ttctgattgc tggtctgttg
     1501 ccgtggtggt cacagtggat cctgaatgtg ccgtgggcca ttttccacat cctgatcatt
     1561 acgctgcaag ccttcatctt catggttctg acgatcgtct atctgtcgat ggcgtctgaa
     1621 gaacattaat ttaccaacac tactacgttt taactgaaac aaactggaga ctgtcatgga
     1681 aaacctgaat atggatctgc tgtacatggc tgccgctgtg atgatgggtc tggcggcaat
     1741 cggtgctgcg atcggtatcg gcatcctcgg gggtaaattc ctggaaggcg cagcgcgtca
     1801 acctgatctg attcctctgc tgcgtactca gttctttatc gttatgggtc tggtggatgc
     1861 tatcccgatg atcgctgtag gtctgggtct gtacgtgatg ttcgctgtcg cgtagtaagc
     1921 gttgctttta tttaaagagc aatatcagaa cgttaactaa atagaggcat tgtgctgtga
     1981 atcttaacgc aacaatcctc ggccaggcca tcgcgtttgt cctgttcgtt ctgttctgca
     2041 tgaagtacgt atggccgcca ttaatggcag ccatcgaaaa acgtcaaaaa gaaattgctg
     2101 acggccttgc ttccgcagaa cgagcacata aggaccttga ccttgcaaag gccagcgcga
     2161 ccgaccagct gaaaaaagcg aaagcggaag cccaggtaat catcgagcag gcgaacaaac
     2221 gccgctcgca gattctggac gaagcgaaag ctgaggcaga acaggaacgt actaaaatcg
     2281 tggcccaggc gcaggcagaa attgaagccg agcgtaaacg tgcccgtgaa gagctgcgta
     2341 agcaagttgc tatcctggct gttgctggcg ccgagaagat catcgaacgt tccgtggatg
     2401 aagctgctaa cagcgacatc gtggataaac ttgtcgctga actgtaagga gggaggggct
     2461 gatgtctgaa tttattacgg tagctcgccc ctacgccaaa gcagcttttg actttgccgt
     2521 cgaacaccaa agtgtagaac gctggcagga catgctgacg tttgccgccg aggtaaccaa
     2581 aaacgaacaa atggcagagc ttctctctgg cgcgcttgcg ccagaaacgc tcgccgagtc
     2641 gtttatcgca gtttgtggtg agcaactgga cgaaaacggt cagaacctga ttcgggtaat
     2701 ggctgaaaat ggtcgtctta acgcgctccc ggatgttctg gagcagttta ttcaccttcg
     2761 tgccgtgagt gaggctaccg ctgaggtaga cgtcatttcc gctgccgcac tgagtgaaca
     2821 acagctcgcg aaaatttctg ctgcgatgga aaaacgtctg tcacgcaaag ttaagctgaa
     2881 ttgcaaaatc gataagtctg taatggctgg cgttatcatc cgagcgggtg atatggtcat
     2941 tgatggcagc gtacgcggtc gtcttgagcg ccttgcagac gtcttgcagt cttaagggga
     3001 ctggagcatg caactgaatt ccaccgaaat cagcgaactg atcaagcagc gcattgctca
     3061 gttcaatgtt gtgagtgaag ctcacaacga aggtactatt gtttctgtaa gtgacggtgt
     3121 tatccgcatt cacggcctgg ccgattgtat gcagggtgaa atgatctccc tgccgggtaa
     3181 ccgttacgct atcgcactga acctcgagcg cgactctgta ggtgcggttg ttatgggtcc
     3241 gtacgctgac cttgccgaag gcatgaaagt taagtgtact ggccgtatcc tggaagttcc
     3301 ggttggccgt ggcctgctgg gccgtgtggt taacactctg ggtgcaccaa tcgacggtaa
     3361 aggtccgctg gatcacgacg gcttctctgc tgtagaagca atcgctccgg gcgttatcga
     3421 acgtcagtcc gtagatcagc cggtacagac cggttataaa gccgttgact ccatgatccc
     3481 aatcggtcgt ggtcagcgtg aattgatcat cggtgaccgt cagacaggta aaaccgcact
     3541 ggctatcgat gccatcatca accagcgcga ttccggtatc aaatgtatct atgtcgctat
     3601 cggccagaaa gcgtccacca tttctaacgt ggtacgtaaa ctggaagagc acggcgcact
     3661 ggctaacacc atcgttgtgg tagcaaccgc gtctgaatcc gctgcactgc aatacctggc
     3721 accgtatgcc ggttgcgcaa tgggcgaata cttccgtgac cgcggtgaag atgcgctgat
     3781 catttacgat gacctgtcta aacaggctgt tgcttaccgt cagatctccc tgctgctccg
     3841 tcgtccgcca ggacgtgaag cattcccggg cgacgttttc tacctccact ctcgtctgct
     3901 ggagcgtgct gcacgtgtta acgccgaata cgttgaagcc ttcaccaaag gtgaagtgaa
     3961 agggaaaacc ggttctctga ccgcactgcc gattatcgaa actcaggcgg gtgacgtttc
     4021 tgcgttcgtt ccgaccaacg taatctccat taccgatggt cagatcttcc tggaaaccaa
     4081 cctgttcaac gccggtattc gtcctgcggt taacccgggt atttccgtat cccgtgttgg
     4141 tggtgcagca cagaccaaga tcatgaaaaa actgtccggt ggtatccgta ccgctctggc
     4201 acagtatcgt gaactggcag cgttctctca gtttgcatcc gaccttgacg atgcaacacg
     4261 taagcagctt gaccacggtc agaaagtgac cgaactgctg aaacagaaac agtatgcgcc
     4321 gatgtccgtt gcgcagcagt ctctggttct gttcgcagca gaacgtggtt acctggcgga
     4381 tgttgaactg tcgaaaattg gcagcttcga agccgctctg ctggcttacg tcgaccgtga
     4441 tcacgctccg ttgatgcaag agatcaacca gaccggtggc tacaacgacg aaatcgaagg
     4501 caagctgaaa ggcatcctcg attccttcaa agcaacccaa tcctggtaac gtctggaggc
     4561 ttgccttagg gcaggccgca aggcattgag gagaagctca tggccggcgc aaaagagata
     4621 cgtagtaaga tcgcaagcgt ccagaacacg caaaagatca ctaaagcgat ggagatggtc
     4681 gccgcttcca aaatgcgtaa atcgcaggat cgcatggcgg ccagccgtcc ttatgcagaa
     4741 accatgcgca aagtgattgg tcaccttgca cacggtaatc tggaatataa gcacccttac
     4801 ctggaagacc gcgacgttaa acgcgtgggc tacctggtgg tgtcgaccga ccgtggtttg
     4861 tgcggtggtt tgaacattaa cctgttcaaa aaactgctgg cggaaatgaa gacctggacc
     4921 gacaaaggcg ttcaatgcga cctcgcaatg atcggctcga aaggcgtgtc gttcttcaac
     4981 tccgtgggcg gcaatgttgt tgcccaggtc accggcatgg gggataaccc ttccctgtcc
     5041 gaactgatcg gtccggtaaa agtgatgttg caggcctacg acgaaggccg tctggacaag
     5101 ctttacattg tcagcaacaa atttattaac accatgtctc aggttccgac catcagccag
     5161 ctgctgccgt taccggcatc agatgatgat gatctgaaac ataaatcctg ggattacctg
     5221 tacgaacccg atccgaaggc gttgctggat accctgctgc gtcgttatgt cgaatctcag
     5281 gtttatcagg gcgtggttga aaacctggcc agcgagcagg ccgcccgtat ggtggcgatg
     5341 aaagccgcga ccgacaatgg cggcagcctg attaaagagc tgcagttggt atacaacaaa
     5401 gctcgtcagg ccagcattac tcaggaactc accgagatcg tctcgggggc cgccgcggtt
     5461 taaacaggtt atttcgtaga ggatttaaga tggctactgg aaagattgtc caggtaatcg
     5521 gcgccgtagt tgacgtcgaa ttccctcagg atgccgtacc gcgcgtgtac gatgctcttg
     5581 aggtgcaaaa tggtaatgag cgtctggtgc tggaagttca gcagcagctc ggcggcggta
     5641 tcgtgcgtac catcgcaatg ggttcctccg acggtctgcg tcgcggtctg gatgtaaaag
     5701 acctcgaaca cccgatcgaa gtcccggtag gtaaagcgac tctgggccgt atcatgaacg
     5761 tactgggtga accggtcgac atgaaaggcg agatcggtga agaagagcgt tgggcgattc
     5821 accgcgcagc accttcctac gaagagctgt caaactctca ggaactgctg gaaaccggta
     5881 tcaaagttat cgacctgatg tgtccgttcg ctaagggcgg taaagttggt ctgttcggtg
     5941 gtgcgggtgt aggtaaaacc gtaaacatga tggagctcat tcgtaacatc gcgatcgagc
     6001 actccggtta ctctgtgttt gcgggcgtag gtgaacgtac tcgtgagggt aacgacttct
     6061 accacgaaat gaccgactcc aacgttatcg acaaagtatc cctggtgtat ggccagatga
     6121 acgagccgcc gggaaaccgt ctgcgcgttg ctctgaccgg tctgaccatg gctgagaaat
     6181 tccgtgacga aggtcgtgac gttctgctgt tcgttgacaa catctatcgt tacaccctgg
     6241 ccggtacgga agtatccgca ctgctgggcc gtatgccttc agcggtaggt tatcagccga
     6301 ccctggcgga agagatgggc gttctgcagg aacgtatcac ctccaccaaa actggttcta
     6361 tcacctccgt acaggcggta tacgtacctg cggatgactt gactgacccg tctccggcaa
     6421 ccacctttgc gcaccttgac gcaaccgtgg tactgagccg tcagatcgcg tctctgggta
     6481 tctacccggc cgttgacccg ctggactcca ccagccgtca gctggacccg ctggtggttg
     6541 gtcaggaaca ctacgacacc gcgcgtggcg ttcagtccat cctgcaacgt tatcaggaac
     6601 tgaaagacat catcgccatc ctgggtatgg atgaactgtc tgaagaagac aaactggtgg
     6661 tagcgcgtgc tcgtaagatc cagcgcttcc tgtcccagcc gttcttcgtg gcagaagtat
     6721 tcaccggttc tccgggtaaa tacgtctccc tgaaagacac catccgtggc tttaaaggca
     6781 tcatggaagg cgaatacgat cacctgccgg agcaggcgtt ctacatggtc ggttccatcg
     6841 aagaagctgt ggaaaaagcc aaaaaacttt aacgccttaa tcggagggtg atatggcaat
     6901 gacttaccac ctggacgtcg tcagcgcaga gcaacaaatg ttctctggtc tggtcgagaa
     6961 aatccaggta acgggtagcg aaggtgaact ggggatctac cctggccacg caccgctgct
     7021 caccgccatt aagcctggta tgattcgcat cgtgaaacag cacggtcacg aagagtttat
     7081 ctatctgtct ggcggcattc ttgaagtgca gcctggcaac gtgaccgttc tggccgacac
     7141 cgcaattcgc ggccaggatc tcgacgaagc gcgagccatg gaagcgaaac gtaaggctga
     7201 agagcacatt agcagctctc acggcgacgt agattacgct caggcgtctg cggaactggc
     7261 caaagcgatc gcgcagctgc gcgttatcga gttgaccaaa aaagcgatgt aacaccggct
     7321 tgaaaagcac aaaagccagt ctggaaacag gctggctttt tttacgcgtt gacccgtcct
     7381 gaagagcgtt cacatagatc ctgctgatat aaaacacccc tgttttcccg tttattcatt
     7441 gatcgaaata agagcaaaaa catccacctg acgcttaaat gaaggtactg ccttaatttt
     7501 ctgcagataa aaggcgtgac gatggtcgaa aatggcactt tcgtcagcgg ggataatccg
     7561 ttattgaaca atttatcctc tgtccatttc acgatgaaaa aaatgtagtt ttttcaaggt
     7621 gaagcggttt aaattcgttc tcaaattaca gtcaggacgc gtatgttgaa taatgctatg
     7681 agcgtagtga tccttgccgc aggcaaaggc acgcgcatgt attccgatct tccgaaagtg
     7741 ctgcataccc ttgccgggaa agcgatggtt cagcatgtca ttgatgctgc gaatgaatta
     7801 ggcgcagcgc acgttcacct ggtgtacggt cacggcggcg atctgcttaa acaggcgctg
     7861 aaagacgaca accttaactg ggtgcttcag gcagagcagc tgggtacggg tcatgcaatg
     7921 cagcaggccg cacctttctt tgccgatgat gaagacattt taatgctcta cggcgacgtg
     7981 ccgctgatct ctgtcgaaac actccagcgc ctgcgtgatg ctaaacccca gggtggcatt
     8041 ggtctgctga cggtgaaact ggatgatccg accggttatg gacgtatcac ccgtgaaaat
     8101 ggcaaagtta ccggcattgt tgagcacaaa gatgccaccg acgagcagcg tcagattcag
     8161 gagatcaaca ccggcattct gattgccaac ggcgcagata tgaaacgctg gctggcgaag
     8221 ctgaccaaca ataatgctca gggcgaatac tacatcaccg acattattgc gctggcgtat
     8281 caggaagggc gtgaaatcgt cgccgttcat ccgcaacgtt taagcgaagt agaaggtgtg
     8341 aataaccgcc tgcaactctc ccgtctggag cgcgtttacc agtccgaaca ggctgaaaaa
     8401 ctgctgttag caggcgttat gctgcgcgat ccggcgcgtt ttgatctgcg cggtacgctt
     8461 actcacgggc gcgatgttga aattgatact aacgttatca tcgagggcaa cgtgactctc
     8521 ggtcatcgcg tgaaaatcgg caccggttgc gtgattaaaa acagcgtgat tggcgatgat
     8581 tgcgaaatca gtccgtatac cgttgtggaa gacgcgaatc tggcagcggc ctgtactatt
     8641 ggcccgtttg cccgtctgcg tcctggtgct gagttgctgg aaggtgcaca cgttggtaac
     8701 ttcgttgaga tgaaaaaagc gcgtctgggt aaaggcacga aagccgggca tctgacttac
     8761 ctgggcgatg cggaaattgg cgataacgtt aacatcggcg cgggaaccat tacctgcaac
     8821 tacgatggtg cgaataaatt taagaccatt atcggcgacg atgtgtttgt cggttccgac
     8881 actcagctgg tggccccggt aacagtaggc aaaggcgcga ccattgctgc gggtacaact
     8941 gtgacgcgta atgtcggcga aaatgcatta gctatcagcc gtgtgccgca gactcagaaa
     9001 gaaggctggc gtcgtccggt aaagaaaaag taattctggc cgggtaatcc ggtcatctgg
     9061 gatgaggaga taacataatc tccctcccac aagcagtaac tataaaaata accccactct
     9121 ctacaaggct cggggcgccc gaaaaaacgg gcatacaggt tgaccgacaa cgatataaat
     9181 cggaatcaaa aactatgtgt ggaattgttg gcgcgatcgc gcaacgtgat gtagcagaaa
     9241 tccttcttga aggtttacgt cgtctggaat accgcggata tgactctgcc ggtctggccg
     9301 ttgttgatgc ggaaggtcat atgactcgcc tgcgtcgcct cggtaaagtc cagatgctgg
     9361 ctcaggcagc ggaagaacat cctctgcatg gcggcaccgg tattgctcac actcgctggg
     9421 cgacccacgg tgaaccttca gaagctaacg cgcatccgca tgtttctgaa cacattgtgg
     9481 tggtgcataa tggcatcatc gaaaaccatg aaccgctgcg tgaagagtta aaagcgcgtg
     9541 gctatacctt cgtttctgaa accgacaccg aagtgattgc ccatctggtg aactgggagc
     9601 tgaaacaagg cgggactctg cgtgaggctg ttctgcgtgc tatcccgcag ctgcgcggtg
     9661 cgtacggtac agtgatcatg gactcccgtc acccggatac cctgctagcg gcacgttctg
     9721 gtagtccgct ggtgattggc ctgggtatgg gcgaaaactt tatcgcttct gaccagctgg
     9781 cgctgttgcc ggtgacccgt cgctttatct tccttgaaga gggcgatatt gcggaaatca
     9841 ctcgccgttc ggtaaacatc ttcgataaaa ctggcgcgga agtaaaacgt caggatatcg
     9901 aatccaatct gcaatatgac gcgggcgata aaggtattta ccgtcactac atgcagaaag
     9961 agatctacga acagccgaac gcgataaaaa acacccttac cgggcgcatc agccacggtc
    10021 aggttgattt aagcgagctg ggaccgaacg ccgacgaact gctgtcgaag gttgagcata
    10081 ttcagatcct cgcctgtggt acttcttata actccggtat ggtttcccgc tactggtttg
    10141 aatcgctggc aggtattccg tgcgacgtcg aaatcgcctc tgaattccgc tatcgtaaat
    10201 ctgccgtgcg tcgtaacagc ctgatgatca ccttgtcaca gtctggcgaa accgcggata
    10261 ccctggcggg cctgcgtctg tcgaaagagc tgggttacct tggttcgctg gcaatctgta
    10321 acgttccggg ttcttctctg gtgcgcgaat ccgatctggc gctgatgacc aacgcgggta
    10381 cggaaatcgg cgtggcgtcc actaaagcat tcaccactca gttaactgtg ctgttgatgc
    10441 tggtggcgaa gctgtctcgc ctgaaaggtc tggatgcctc cattgaacat gacattgtgc
    10501 atggtctgca ggcgctgccg agccgtattg agcagatgct gtctcaggat aaacgcattg
    10561 aagctctggc agaagatttc tctgacaaac atcacgcgct gttcctgggc cgtggcgatc
    10621 agtacccaat cgcgctggaa ggcgcattga agctgaaaga gatctcttac attcacgctg
    10681 aagcctacgc tgcaggcgag ctgaaacacg gcccgctggc gctgattgac gcggatatgc
    10741 cggttatcgt cgttgcaccg aacaacgaac tgttggaaaa actgaaatcc aacattgaag
    10801 aagttcgcgc gcgtggcggt caattgtatg tcttcgccga tcaggatgcg ggttttgtaa
    10861 gtagcgataa catgcacatc atcgagatgc cgcatgtgga agaggtgatt gcaccgatct
    10921 tctacaccgt tccgctgcag ctgctggctt accatgtcgc gctgatcaaa ggcaccgacg
    10981 ttgaccagcc gcgtaacctg gcaaaatcgg ttacggttga gtaatcacta tcatagataa
    11041 aaaagggctg ctgtgggcgg acaataaagt cttaaactga acaaaataga tctaaactat
    11101 gacaataaag tcttaaacta gacagaatag ttgtaaactg aaatcagtcc agttatgctg
    11161 tgaaaaagca tactggactt ttgttatggc taaagcaaac tcttcatttt ctgaagtgca
    11221 aattgcccgt cgtattaaag aggggcgtgg ccaagggcat ggtaaagact atattccatg
    11281 gctaacagta caagaagttc cttcttcagg tcgttcccac cgtatttatt ctcataagac
    11341 gggacgagtc catcatttgc tatctgactt agagcttgct gtttttctca gtcttgagtg
    11401 ggagagcagc gtgctagata tacgcgagca gttcccctta ttacctagtg ataccaggca
    11461 gattgcaata gatagtggta ttaagcatcc tgttattcgt ggtgtagatc aggttatgtc
    11521 tactgatttt ttagtggact gcaaagatgg tccttttgag cagtttgcta ttcaagtcaa
    11581 acctgcagca gccttacaag acgagcgtac cttagaaaaa ctagaactag agcgtcgcta
    11641 ttggcagcaa aagcaaattc cttggttcat ttttactgat aaagaaataa atcccgtagt
    11701 aaaagaaaat attgaatggc tttattcagt gaaaacagaa gaagtttctg cggagctttt
    11761 agcacaacta tccccattgg cccatatcct gcaagaaaaa ggagatgaaa acattatcaa
    11821 tgtctgtaag caggttgata ttgcttatga tttggagtta ggcaaaacat tgagtgagat
    11881 acgagcctta accgcaaatg gttttattaa gttcaatatt tataagtctt tcagggcaaa
    11941 taagtgtgca gatctctgta ttagccaagt agtgaatatg gaggagttgc gctatgtggc
    12001 aaattaatga ggttgtgcta tttgataatg atccgtatcg cattttggct atagaggatg
    12061 gccaagttgt ctggatgcaa ataagcgctg ataaaggagt tccacaagct agggctgagt
    12121 tgttgctaat gcagtattta gatgaaggcc gcttagttag aactgatgac ccttatgtac
    12181 atcttgattt agaagagccg tctgtagatt ctgtcagctt ccagaagcgc gaggaggatt
    12241 atcgaaaaat tcttcctatt attaatagta aggatcgttt cgaccctaaa gtcagaagcg
    12301 aactcgttga gcatgtggtc caagaacata aggttactaa ggctacagtt tataagttgt
    12361 tacgccgtta ctggcagcgt ggtcaaacgc ctaatgcatt aattcctgac tacaaaaaca
    12421 gcggtgcacc aggggaaaga cgttcagcga caggaacagc aaagattggc cgagccagag
    12481 aatatggtaa gggtgaagga accaaggtaa cgcccgagat tgaacgcctt tttaggttga
    12541 ccatagaaaa gcacctgtta aatcaaaaag gtacaaagac caccgttgcc tatagacgat
    12601 ttgtggactt gtttgctcag tattttcctc gcattcccca agaggattac ccaacactac
    12661 gtcagtttcg ttatttttat gatcgagaat accctaaagc tcagcgctta aagtctagag
    12721 ttaaagcagg ggtatataaa aaagacgtac gacccttaag tagtacagcc acttctcagg
    12781 cgttaggccc tgggagtcgt tatgagattg atgccacgat tgctgatatt tatttagtgg
    12841 atcatcatga tcgccaaaaa atcataggaa gaccaacgct ttacattgtg attgatgtgt
    12901 ttagtcggat gatcacgggc ttttatatcg gctttgaaaa tccgtcttat gtggtggcga
    12961 tgcaggcttt tgtaaatgct tgctctgaca aaacggccat ttgtgcccag catgatattg
    13021 agattagtag ctcagactgg ccgtgtgtag gtttgccaga tgtgttgcta gcggaccgtg
    13081 gcgaattaat gagtcatcag gtcgaagcct tagtttctag ttttaatgtg cgagtggaaa
    13141 gtgctccacc tagacgtggc gatgctaaag gcatagtgga aagcactttt agaacactac
    13201 aagccgagtt taagtccttt gcacctggca ttgtagaggg cagtcggatc aaaagccatg
    13261 gtgaaacaga ctataggtta gatgcatctc tgtcggtatt tgagttcaca caaattattt
    13321 tgcgtacgat cttattcaga aataaccatc tggtgatgga taaatacgat cgagatgctg
    13381 attttcctac agatttaccg tctattcctg tccagctatg gcaatggggt atgcagcatc
    13441 gtacaggtag tttaagggct gtggagcaag agcagttgcg agtagcgtta ctgcctcgcc
    13501 gaaaggtctc tatttcttca tttggcgtta atttgtgggg tttgtattac tcggggtcag
    13561 agattctgcg tgagggttgg ttgcagcgga gcactgatat agctagacct caacatttag
    13621 aagcggctta tgacccagtg ctggttgata cgatttattt gtttccgcaa gttggcagcc
    13681 gtgtattttg gcgctgtaat ctgacggaac gtagtcggca gtttaaaggt ctctcatttt
    13741 gggaggtttg ggatatacaa gcacaagaaa aacacaataa agccaatgcg aagcaggatg
    13801 agttaactaa acgcagggag cttgaggcgt ttattcagca aaccattcag aaagcgaata
    13861 agttaacgcc cagtactact gagcccaaat caacacgcat taagcagatt aaaactaata
    13921 aaaaagaagc cgtgacctcg gagcgtaaaa aacgtgcgga gcatttgaag ccaagctctt
    13981 caggtgatga ggctaaagtt attcctttca acgcagtgga agcggatgat caagaagatt
    14041 acagcctacc cacatacgtg cctgaattat ttcaggatcc accagaaaag gatgagtcat
    14101 gagtgctacc cggattcaag cagtttatcg tgatacgggg gtagaggctt atcgtgataa
    14161 tccttttatc gaggccttac caccattaca agagtcagtg aatagtgctg catcactgaa
    14221 atcctcttta cagcttactt cctctgactt gcaaaagtcc cgtgttatca gagctcatac
    14281 catttgtcgt attccagatg actattttca gccattaggt acgcatttgc tactaagtga
    14341 gcgtatttcg gtcatgattc gaggtggcta cgtaggcaga aatcctaaaa caggagattt
    14401 acaaaagcat ttacaaaatg gttatgagcg tgttcaaacg ggagagttgg agacatttcg
    14461 ctttgaggag gcacgatcta cggcacaaag cttattgtta attggttgtt ctggtagtgg
    14521 gaagacgacc tctcttcatc gtattctagc cacgtatcct caggtgattt accatcgtga
    14581 actcaatgta gagcaggtgg tgtatttgaa aatagactgc tcgcataatg gttcgctaaa
    14641 agaaatctgc ttgaattttt tcagagcgtt ggatcgagcc ttgggctcga actatgagcg
    14701 tcgttatggc ttaaaacgtc atggtataga aaccatgttg gctttgatgt cgcaaatagc
    14761 caatgcacat gctttagggt tgttggttat tgatgaaatt cagcatttaa gccgctctcg
    14821 ttcgggtgga tctcaagaga tgctgaactt ttttgtgacg atggtgaata ttattggcgt
    14881 accagtgatg ttgattggta cccctaaagc acgagagatt tttgaggctg atttgcggtc
    14941 tgcacgtaga ggggcagggt ttggagctat attctgggat cctatacaac aaacgcaacg
    15001 tggaaagccc aatcaagagt ggatcgcttt tacggataat ctttggcaat tacagctttt
    15061 acaacgcaaa gatgcgctgt tatcggatga ggtccgtgat gtgtggtatg agctaagcca
    15121 aggagtgatg gacattgtag taaaactttt tgtactcgct cagctccgtg cgctagcttt
    15181 aggcaatgag cgtattaccg ctggtttatt gcggcaagtg tatcaagatg agttaaagcc
    15241 tgtgcacccc atgctagagg cattacgctc gggtatccca gaacgcattg ctcgttattc
    15301 tgatctagtc gttcccgaga ttgataaacg gttaatccaa cttcagctag atatcgcagc
    15361 gatacaagaa caaacaccag aagaaaaagc ccttcaagag ttagataccg aagatcagcg
    15421 tcatttatat ctgatgctga aagaggatta cgattcaagc ctgttaattc ccactattaa
    15481 aaaagcgttt agccagaatc caacgatgac aagacaaaag ttactgcctc ttgttttgca
    15541 gtggttgatg gaaggcgaaa cggtagtgtc agaactagaa aagccctcca agagtaaaaa
    15601 ggtttcggct ataaaggtag tcaagcccag cgactgggat agcttgcctg atacggattt
    15661 acgttatatc tattcacaac gccaacctga aaaaaccatg catgaacggt taaaagggaa
    15721 aggggtaata gtggatatgg cgagcttatt taaacaagca ggttagccat gagaaacttt
    15781 cctgttccgt actcgaatga gctgatttat agcactattg cacgggcagg cgtttatcaa
    15841 gggattgtta gtcctaagca gctgttggat gaggtgtatg gcaaccgcaa ggtggtcgct
    15901 accttaggtc tgccctcgca tttaggtgtg atagcaagac atctacatca aacaggacgt
    15961 tacgctgttc agcagcttat ttatgagcat accttattcc ctttatatgc tccgtttgta
    16021 ggcaaggagc gccgagacga agctattcgg ttaatggagt accaagcgca aggtgcggtg
    16081 catttaatgc taggagtcgc tgcttctaga gttaagagcg ataaccgctt tagatactgc
    16141 cctgattgcg ttgctcttca gctaaatagg tatggggaag ccttttggca acgagattgg
    16201 tatttgcccg ctttgccata ttgtccaaaa cacggtgctt tagtcttctt tgatagagct
    16261 gtagatgatc accgacatca attttgggct ttgggtcata ctgagctgct ttcagactac
    16321 cccaaagact ccctatctca attaacagca ctagctgctt atatagcccc tctgttagat
    16381 gctccacgag cgcaagagct ttccccaagc cttgagcagt ggacgctgtt ttatcagcgc
    16441 ttagcgcagg atctagggct aaccaaaagc aagcacattc gtcatgactt ggtggcggag
    16501 agagtgaggc agacttttag tgatgaggca ctagagaaac tggatttaaa gttggcagag
    16561 aacaaggaca cgtgttggct gaaaagtata ttccgtaagc atagaaaagc ctttagttat
    16621 ttacagcata gtattgtgtg gcaagcctta ttgccaaaac taacggttat agaagcgcta
    16681 cagcaggcaa gtgctcttac tgagcactct ataacgacaa gacctgttag ccagtctgtg
    16741 caacctaact ctgaagattt atctgttaag cataaagact ggcagcaact agtgcataaa
    16801 taccaaggaa ttaaggcggc aagacagtct ttagagggtg gggtgctata cgcttggctt
    16861 taccgacatg acagggattg gctagttcac tggaatcaac agcatcaaca agagcgtctg
    16921 gcacccgccc ctagagttga ttggaaccaa agagatcgaa ttgctgtacg acaactatta
    16981 agaatcataa agcgtctaga tagtagcctt gatcacccaa gagcgacatc gagctggctg
    17041 ttaaagcaaa ctcctaacgg aacctctctt gcaaaaaatc tacagaaact gcctttggta
    17101 gcgctttgct taaagcgtta ctcagagagt gtggaagatt atcaaattag acggattagc
    17161 caagctttta ttaagcttaa acaggaagat gttgagctta ggcgctggcg attattaaga
    17221 agtgcaacgt tatctaaaga gcggataact gaggaagcac aaagattctt ggaaatggtt
    17281 tatggggaag agtgagtggt taggctagct acatttaatg acaatgtgca ggttgtacat
    17341 attggtcatt tattccgtaa ctcgggtcat aaggagtggc gtatttttgt ttggtttaat
    17401 ccaatgcaag aacggaaatg gactcgattt actcatttgc ctttattaag tcgagctaag
    17461 gtggttaaca gtacaacaaa gcaaataaat aaggcggatc gtgtgattga gtttgaagca
    17521 tcggatcttc aacgagccaa aataatcgat tttcctaatc tctcgtcctt tgcttccgta
    17581 cgcaacaagg atggagcgca gagttcattt atttacgaag ctgaaacacc atatagcaag
    17641 actcgttatc acatcccaca gttagagcta gctcggtcat tatttttaat taactcctat
    17701 ttctgtcgaa gctgtttgag cagtaccgct ttacagcaag agttcgacgt tcagtatgag
    17761 gttgagcgag atcatttaga gataaggatc ttacccagtt catcgtttcc taaaggggcg
    17821 ttagagctgt cggccgtagt gcagcttttg gtttggttgt tttcggatca agatgttatg
    17881 gattcgtatg aaagtatttt taggcactat caacaaaata gagaaattaa gaacggcgtt
    17941 gaaagctggt gctttagctt tgaccctccg cccatgcagg gttggaaatt acatgtaaaa
    18001 ggacgttctt ctaacgagga taaggattat ttagttgagg aaatagtagg tttagaaatc
    18061 aacgctatgc ttcctagcac aacagctatt agccatgcct cttttcagga aaaggaggca
    18121 ggtgatggta gtacgcagca catagcggtt tcaacagagt cagttgttga tgatgagcat
    18181 ctacagttgg acgatgagga aacagccaat atagacacag acacacgagt catagaggct
    18241 gagccgacat ggataagttt tagtagacct agtcgaattg aaaaatctcg cagggcaaga
    18301 aaaagtagcc aaactatttt agaaaaagaa gaagcaacaa caagtgaaaa tagtaatttg
    18361 gttagtactg atgagccaca cttaggtggt gtcctagcag cggcagatgt gggtgggaag
    18421 caggatgcaa ccaattacaa ctctattttt gctaatcgat ttgctgcttt tgatgagcta
    18481 ctttcaattc taaaaactaa atttgcatgt cgggtgcttt ttgaagaaac cttggttttg
    18541 ccaaaagttg ggcgtagccg attacatctg tgtaaagatg gctcaccaag agtgattaaa
    18601 gccgttgggg tgcaacgtaa tggcagtgaa tttgtattgc tagaggtgga tgcatcggat
    18661 ggggtgaaaa tgctttctac caaagtgttg agtggcgttg atagcgaaac atggcggaat
    18721 gattttgaaa agatacggcg tggagtggtg aagagctcat tgaattggcc aaatagtttg
    18781 tttgatcaat tatatggaca agacgggcat agaggggtga atcatccaaa ggggttgggg
    18841 gagctgcaag tatcgagaga ggatatggaa gggtgggctg agagagtggt tagagagcaa
    18901 tttacgcatt aaaggaatga ctgaaagagc ctgtaaaccc ttttgtgtaa gtgcttttgc
    18961 cggtcagtta aaggtggcca tttaaacggt caccaaattc gatcataaaa cggttcatgg
    19021 ccggcttcca gttgcggatc ggcatcgtcc atttcttggt cgccgcctgg atagccaggt
    19081 acaccacctt catcgctgat tcgtccgtag ggaacacctt gagttcatat taatggaatt
    19141 ttctacaaat agcctccgtg gttttgaggg gggattacag acgatccata gtagtaatcc
    19201 aatgagttct tgagcgcggc gacatgtttg gacgccttgg caaaaattag agcctgcttg
    19261 aagtgcaggc gagcacgtgc ttggacgatt gatccattcg cggtcaaaaa ctcaatcttg
    19321 gatgacagcg tgtcaggaaa tccaatatcg aagtcccgcc ccgtgtcaaa gtagggcatt
    19381 tcatgatcaa aggacgaagc ttcccaggcc acttccctaa tattccagcc gctagaaaca
    19441 agatttcgta cgacttcatc aacttttttg ctttccaact tgtcacaaag tgcataggca
    19501 agagctttgg cggcagacac gtgcgtttcc tcttaaacat aggtcgaacg gatacccgag
    19561 gcaaagcgga gcattagtcc caaagcatct ccggtagatc cggaggctcc atactgaagg
    19621 catggcatct tgggcattga tatttgaaac ttctctctag atagcaggga tttcttggcc
    19681 ttgcctcatg ctgatagctt gtaaatatca cacgatcctt agccccacaa agtgagcata
    19741 ctccgtagct ttcatttata gaaatcgtcg ttagattgtc gcagcatgtg cacgttccga
    19801 ctatttgctc aggaagcatc
//
LOCUS       DAZPCS010000064        19698 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZPCS010000064 DAZPCS010000000
VERSION     DAZPCS010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19698
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.064"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(151..1584)
                     /gene="hldE"
                     /locus_tag="ACHUQ7_002202"
     CDS             complement(151..1584)
                     /gene="hldE"
                     /locus_tag="ACHUQ7_002202"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFR1135602.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVFYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="ACHUQ7_002203"
     CDS             complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="ACHUQ7_002203"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFR1135603.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTMIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(4495..5796)
                     /gene="ygiF"
                     /locus_tag="ACHUQ7_002204"
     CDS             complement(4495..5796)
                     /gene="ygiF"
                     /locus_tag="ACHUQ7_002204"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFR1135604.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQSLFSTDFYREKWLVEIDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            6038..6658
                     /locus_tag="ACHUQ7_002205"
     CDS             6038..6658
                     /locus_tag="ACHUQ7_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFR1135605.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            6722..7960
                     /gene="cca"
                     /locus_tag="ACHUQ7_002206"
     CDS             6722..7960
                     /gene="cca"
                     /locus_tag="ACHUQ7_002206"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFR1135606.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYTAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIRTFPMLNPKTIVKLFDSIDAWRKPQR
                     IEQLALTSEADVRGRTGFESADYPQGRWLREGWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(8123..8944)
                     /gene="bacA"
                     /locus_tag="ACHUQ7_002207"
     CDS             complement(8123..8944)
                     /gene="bacA"
                     /locus_tag="ACHUQ7_002207"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFR1135607.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(9035..9403)
                     /gene="folB"
                     /locus_tag="ACHUQ7_002208"
     CDS             complement(9035..9403)
                     /gene="folB"
                     /locus_tag="ACHUQ7_002208"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFR1135608.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            9508..10125
                     /gene="plsY"
                     /locus_tag="ACHUQ7_002209"
     CDS             9508..10125
                     /gene="plsY"
                     /locus_tag="ACHUQ7_002209"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFR1135609.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPISWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(10068..11069)
                     /locus_tag="ACHUQ7_002210"
     CDS             complement(10068..11069)
                     /locus_tag="ACHUQ7_002210"
                     /inference="COORDINATES: protein motif:HMM:NF007315.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1135610.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     ELLYRSMKFVSARNFWRHGASNGWASPMKAIRSCRSTRNHSGSFSRFLLNFVQIFVS"
     gene            11276..12119
                     /gene="ttdA"
                     /locus_tag="ACHUQ7_002211"
                     /pseudo
     CDS             11276..12119
                     /gene="ttdA"
                     /locus_tag="ACHUQ7_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            12116..12721
                     /gene="ttdB"
                     /locus_tag="ACHUQ7_002212"
     CDS             12116..12721
                     /gene="ttdB"
                     /locus_tag="ACHUQ7_002212"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFR1135611.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            12769..14232
                     /gene="ttdT"
                     /locus_tag="ACHUQ7_002213"
     CDS             12769..14232
                     /gene="ttdT"
                     /locus_tag="ACHUQ7_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFR1135612.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVTLIVVFYLLRY
                     FFASATAYTCALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(14275..15288)
                     /gene="tsaD"
                     /locus_tag="ACHUQ7_002214"
     CDS             complement(14275..15288)
                     /gene="tsaD"
                     /locus_tag="ACHUQ7_002214"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFR1135613.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            15526..15741
                     /gene="rpsU"
                     /locus_tag="ACHUQ7_002215"
     CDS             15526..15741
                     /gene="rpsU"
                     /locus_tag="ACHUQ7_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFR1135614.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            15852..17597
                     /gene="dnaG"
                     /locus_tag="ACHUQ7_002216"
     CDS             15852..17597
                     /gene="dnaG"
                     /locus_tag="ACHUQ7_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFR1135615.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            17792..19633
                     /gene="rpoD"
                     /locus_tag="ACHUQ7_002217"
     CDS             17792..19633
                     /gene="rpoD"
                     /locus_tag="ACHUQ7_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFR1135616.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         4683 a         5506 c         5031 g         4478 t
ORIGIN      
        1 gttgttactg ttaagtttcc cgacttacta acaactgatc agagggggga gaaatcctcc
       61 cttacccttg ttcctttact ttaggttgaa aaaacaacag cgtcaatagg cctgcctgct
      121 acgaagcgag atctgtgaac cgctttccgg ttagcctttt ttatcctgtt ggatcttctt
      181 gatgatgtta gtggtcgagc aaccgtcttc gaagttgagc accagcactt cgccgccgtt
      241 ggcccagact tctttactcc cggcgatctc ttctggttta tagtcgccgc ccttcaccag
      301 taaatctggc aggatcccgg cgatcaagcg ctgcggcgtg tcttcttcaa acgacaccac
      361 ccagtcgacc gcttccagtg cgcccagcac aatcatacgc tgttcgagcg ggttaacggg
      421 gcgagaatcc cctttcagcc gtttggtgga ggcatcgctg ttcacagcga caatcaagcg
      481 gtcacccagt ttgcgggcat ttgccagata gaagacgtgt ccggcgtgca ggatgtcaaa
      541 gacgccattg gtcatcacca ctttttcacc gcgtttacgc gctgccgcta cggccagctt
      601 cagttcctct tcggtcatca cgccaaagcc ggtatctgcg cgcccacgca ctgcattttc
      661 cagctcgatc ggcgaaaccg tggaggttcc cagtttgccg accaccacgc cagccgccgc
      721 attggcaaag aagcaggctt cttccagcga attacccgct gccagcgttg ccgccaggac
      781 gccaatcacc gtgtcgcccg caccggtaac gtcatacact tcctgcgcct gggttggcat
      841 atgcagcggc gctttacccg gttgcagcag cgacataccc tgttcggaac gggtcactaa
      901 cagagccgag agttcgtaat cggcaatcag tttcatgccg cgctcaacaa tctcttcttc
      961 ggtcttacat ttaccgacaa cagcttcaaa ttccgagaga ttcggtgtca gcagcgtagc
     1021 gccgcggtag cgctcaaaat cggtaccttt tggatcaatc agcaccggaa cgcccgcttt
     1081 acgcgccagt tggatcatct gctgtacgct tgccagcgca cctttggcgt agtcagaaag
     1141 caccagcgcg ccaatcgaac tcagcgcctg attaatccgc tcgtgcagcg gctgcggatc
     1201 aacaccttcg aaaccttctt caaaatccag acggatcagc tgttggttgc gggaaagtac
     1261 ccgtaatttg gtgatggtcg gatgcgtcgg tacagaaacg aagtcgcatt tgacgttgac
     1321 gtcggccaga gatttactca gcgcgcgcgc tgcatcgtca atgcccgtca acccgaccag
     1381 gcgtgcatta gcaccgagag aagcgatatt catcgccacg ttagccgcgc cgcccggacg
     1441 ttcttcgatg gtattcactt taaccacggg caccggcgct tccggcgaga tacgactggt
     1501 ggggccgtac cagtaacgat ccagcatcac atcaccaacc accatcactc ctgcacgttc
     1561 aaactctggc agcgttactt tcattcctgt ctcctgagag attcaaaatt tgcgcgcgat
     1621 aataccatac ttcattcttc caccagccac ttctgccagc ttgcccgcac cagatcacgc
     1681 tctgcggtga agcaatcctc cgacacatgg cccggcaatt cctgtaatgc cagatgatga
     1741 agttcatcgc gcagcgtcgt gtaagcacgg gtcagcgcca tcgcttcctg ctcttccata
     1801 atgtcgtttt gcgccagcag ctccagaata cgcacgttgt ctgaccagcg cgttaacttc
     1861 ggtttttcat gagcgtagcg caacaccaga tattgggtaa taaattcgat atcggtaatt
     1921 ccgccttcat cggctttgat atcaaagcga ttacgatgtt tattgccgag atgagcgcgc
     1981 attttctcgc gcatttcccg cacttccgtt tgcagagttt taccttctcg cggcagtgtc
     2041 ataatctcac gacgcaccgc gtcaaagtgc gcagtgagct gcggatcgcc gtacactaca
     2101 cgcgcacgca ccagcgcctg atgttcccac gtccaggcct cgtttttctg ataatcggca
     2161 aatgcttctg cggatgtcac cagcattccc gccgccccgg acggacgcag tcgagcatcc
     2221 acttcataca aaatgccgga agaggtacgc gtactgaaca gatgcataat gcgttgcgcc
     2281 agacgcagat aaaactgccg cccgtcgatt tcccgctcgc cgtcagtcat cgcatccatt
     2341 gggcaatcat ggaggaagat aaggtcaaga tcggaactgt agcctaactc ccagccgccc
     2401 agcttgccgt agccgaccac cgcaaaaccg cgcccttcac gttcgttgag gtgatttggc
     2461 ttaccgtagc gggccaccat ttgaccccac gcctgctgaa cgacggcatc tatcatagct
     2521 tctgccagcc aggttaagtg atcgctcact ttcattaccg gtagcgtacc ggcgatatcc
     2581 gctgccgcaa tgcgtaacag ctgcgcctgt ttgaactgac gcagcgcctc aagctgttgt
     2641 tcttcgtcat cttccggcac gcgcagcaaa tactggcgca gttcatcacg gtaggcatcg
     2701 gtcgccgtcg gctgataaag ggtatttgga tcgagcaatt catccagcaa taatggataa
     2761 cgcgctagct ggctggcaat catcggcgat gcggcacaca gagaaatcaa atgtttaagc
     2821 gccgcaggga attcactgag caattctaaa taggttgtgc gggtaacaat ccccaccagc
     2881 aaggcggtaa tgcgcgacag cgtgacagca gcgtcttcac gcgcacagac atcacttagc
     2941 agatgtggca tcagatggtc gagcacctga cgtcctcgcg gcccgatagt acgcttatcc
     3001 agctctttgc ggaaatcagc aatcatcgtt aggacctgtt tgcgatcatc ctcgctaaga
     3061 tgcgccagca ctggcgtagt gtcatcttcc tgcaacgcat cctgccacag ctcacgccac
     3121 tgttccgaca gcgactcttc ctgagtttca ctttcgtcgt cgccaatcaa ttcattaaac
     3181 acccggcgca cattggtcat atgtgcggtc agcgccccgg tcagttgcgg ccagtcagca
     3241 aagtccatcg cccacgccag ccgcgcacgg ttaagctcat cagaaggaag cgtttgggtt
     3301 tgttcgtcgt taatgctttg cagcaggttt tccagacgcc gcaggaacag atatgccact
     3361 cgcaattgtt cagcatcgtt ttcagaaagc agatgcagcg cggcaatggc gctgagcgtt
     3421 ggcagtaaag agcgcgactg cagcaacggt tcgcgcccac cgcgaatgag ctggaacacc
     3481 tgaacgataa attcaatttc gcgaatgccg cctgcgccga gtttgatatt gtcggtcaaa
     3541 ccacgtcgac gcacttcacg ggcaatcatc cctttcatgt tgcgcagcga ctgaatcacg
     3601 ctgaaatcga tgtaacgacg gaaaacaaac gggcgcagca tcgcacgcaa ctcgttagca
     3661 tagacgcctt cgctatcgcc cataatccgc gctttgacca tcgcgtaacg ctcccagtcg
     3721 cgtccctgct cctggtaata atcctccaac gcggcaaagc tcagcaccag cgggccactt
     3781 tcgccaaacg gacgcagtcg catatccacg cgatagacga agccatcctg cgttggttga
     3841 tccagcactt taatcagccg ctgccccatg cgggtaaaaa actgcgcgtt atccagttcc
     3901 cggcgtccgc cctgcgtaca accatgttcc ggccaggcaa aaatcagatc gatatcagag
     3961 gagaaattca gttccccgcc gcccagctta cccatgccta agatcagcag cggttgcgct
     4021 tcgccctgcg cattgcacgg cgttccccac tcgcggcagc aggcgtcata cagccagtca
     4081 cgcgccgcaa caatcagcgt ctccgccaga taactgagct gctgcaatat gctctcttca
     4141 gtaaccagtg ccagcgtttg cgcccatgcg atgcgcacca taatgcgccg ccggaatagc
     4201 cgcagctcgc gcattaaccc ggcttcgtcg ctcacattac tgagcgcctc ctgcaaccac
     4261 acgctgtaat gttgccattc gtcagcctgt ggcggttggc tttccagttc cgtcagccac
     4321 tctggatgcg caatcacgct gtcctgcaca aaatcactga aagtaagtac tgacttcgcc
     4381 tgtgcactaa gtgattcctc ggctaaaagc tctggcagcc gctcaacaac ggtctgccag
     4441 tactgctgta acggtgaaga gagcggcttc ataagcgatt ttatccttgc ctgattaacg
     4501 ttttccgctg tgcaaccaga acggttcctg attgtttgcc tcattacgga aatgttcaat
     4561 ttcaatgcgt tgcccggtcg caatagcgtg acgcagcccc tgccagttct ccagccaggc
     4621 ttgcgcgacg acaggatcat agtaacccgc cagcaacagt attgagtcaa tatcacgcat
     4681 caggcgtggc aactggtcac ggtagcgatc gcctaacggc tggcagaaaa cgcttttcag
     4741 ttcagcggca tggcgggaaa gatggatatc ggcaaagcgt ttgaaggagt cgctaatttt
     4801 gccctgcgct ttggcatcta aaaacggctg ccatgctttg cttaccagcc attcggtcaa
     4861 cgccagcttc gccattgccg tttcggtaga gtagaccgcc gtcacggcag aaaccgcaga
     4921 agcgatggtc gcttcgcatt gagtcagcaa atcacgtaag tgagtactcg ctttacgcgg
     4981 cacaatgcca ccgaacagca tcagtgtatg acggactagg ctaatggctg ccagcacctg
     5041 ctctttcgcc gcatcgttgc cgcgtaccca cagttcttca tgatactgcc attgcgctaa
     5101 caccagctcg agcgccgctt ccagcccctg ttccacatcg gcttttgccg caacatgcaa
     5161 aatggtggtc ggtttgattt cacgcgccgg attgccctgc gccagatgat agccacgcgc
     5221 cgctttgctc aggctgccct ggcgtaatcc ggtttgcgat accagttggt tcgccagttt
     5281 cagcaccgcg cgcgtgtcgc cgctaagcag ctccagttcc agctcgcaga taggctcagc
     5341 aaattcaccc gctttcactt ccccctggtc gagagcgatt tcaattcggc tgccatcgat
     5401 ctctactagc catttttcgc gataaaaatc ggtgctaaac agcgactgca cgcgggaagc
     5461 gagatcggca ggcaattcgc cgttcggcca gatttccgtc ggtaactgcg ccaggtcgag
     5521 cgtcggttcg ctcaacgcca cgttatattc cgggcgctgg tgtaagccgc ctgtcactct
     5581 tcctgcaact ttcatggtca tctcatagcg accgttttcg ccacgaatac gtaagcccat
     5641 atcgtgccta cgcagccagt tatccggcgt ttcgtagtaa atattcagca actgcacggg
     5701 gtcatggtgc tcgccgccca gcgtattgag atggtcacgc aacgcctcaa cggcactgtg
     5761 attaacaata aactttaatt cgatttcctg agccatggcc tttacttatc ggttatgtca
     5821 cataagtgac gatgaacggc gaacttaatg cgatcttttt tgtcagtaga tagtattttg
     5881 cgccaaattg ccatgcaacg ggcaatttga cgggcgtaaa agtttgaagc agtggcaaga
     5941 cctgacacag aggattccga ttgatgtcga atgctttgcg tagcgacact gatgccacta
     6001 ctatcgttcc actttccatg acaataacga cagcctgatg ccaaaattac gcctgatcgg
     6061 attaacttta ctcgcactta gcgcgactgc cgtctcacac gctgaagaaa cgcgctatgt
     6121 ttctgacgaa ctgaatacct gggtccgtag cggtccggga gatcattatc gcctcgtggg
     6181 cacggttaac gccggcgagg aagtgacctt attacaaact gacgccaaca ccaattatgc
     6241 ccaggtgaaa gacagctctg gccgtaccgc ctggatcccg ttgaaacaac ttagcactga
     6301 gccaagcctg cgctcccgtg tgccagatct ggaaaatcag gtcaaaaccc tgaccgataa
     6361 actcaccaat atcgataaca cctggaatca gcgcaccgca gaaatgcagc aaaaagtggc
     6421 gcagagcgac agcgtgatca acgggttaaa agaagaaaat cagaaactga aaaacgagct
     6481 gattgtcgcg cagaaaaagg tcgatgccgc cagcgtacag ctggatgaca aacagcgcac
     6541 catcatcatg caatggttta tgtatggcgg tggcgtgctg gggcttggct tgctgctcgg
     6601 tctggtactg ccgcacctga tcccaagccg caaacgcaaa gatcgctgga tgaactaaat
     6661 cgccttcttc gttgcactgt cttattatta ggaattgtgt atttgacgaa gggatgatgg
     6721 cgtgaagatt tatctggtcg gtggtgctgt tcgggatgca ttgttaggac tatcggtcaa
     6781 agacagagat tgggtggtgg tcggcagtac gccacaggag atgctcgacg cgggctacca
     6841 gcaggtaggc cgcgattttc ctgtgtttct gcatccgcaa acgcatgaag agtatgcgct
     6901 ggcacgtacc gaacggaaat ccggttccgg ttacaccggt tttacttgct ataccgcacc
     6961 ggatgtcacg ctggaagatg atcttaagcg tcgcgatctg accattaatg cgctggccca
     7021 ggacgataac ggcgagatta tcgacccgta caacggtctg ggcgatctgc aaaatcgtct
     7081 gttgcgccat gtttcccccg cttttggcga agatccgtta cgcgtactgc gcgtggcgcg
     7141 ttttgctgcg cgttatgccc acctcggttt tcgtattgcc gatgaaactc tgacgctgat
     7201 gcgcgagatg acccatgcgg gtgaactgga acacctgacg cctgaacggg tatggaaaga
     7261 gacggaaagc gccctcacca cccgcaatcc ccaggtgttc ttccaggtac tgcgcgattg
     7321 cggcgcactt cgcgttttat tcccggaaat tgacgcactg tttggcgttc cggcacctgc
     7381 caggtggcat ccggaaatcg acacgggtat tcatacctta atgacgctct caatggcggc
     7441 gatgctgagt ccgcaggttg atgtccgttt cgcaacttta tgccacgatc tcggtaaagg
     7501 actgacgccg ccagaacttt ggccgcgtca tcatgggcat ggcccggcgg gtgttaagtt
     7561 agtggaacaa ctatgccagc gtctgcgcgt gccaaatgaa attcgcgatt tagccagact
     7621 ggtggctgag tttcacgatc tcatccgcac cttcccaatg ctgaacccaa aaaccatcgt
     7681 caaattattt gattccatcg atgcctggcg taaaccgcag cgtatcgagc aactggcgct
     7741 gaccagcgag gctgacgtgc gcggcagaac cggttttgaa tcagcggact acccgcaagg
     7801 ccgttggttg cgcgaaggct gggaagtggc gcagtcagtg ccgacaaaag cggtcgttga
     7861 agcgggattt aaaggcgtgg agattcgcga ggagctgacc cgacggcgga ttgcggcggt
     7921 agccagctgg aaggaacaac gttgcccaaa gcctgactga gtataaaact aaacgttagc
     7981 acgaaccgtc ccctcgcccc ttcggggaga gggttagggt gaggggaaca gaccagcacc
     8041 ggtgcgaaca atcaccccca ccccggccct ctccctacag agcgagggag aaaacccggc
     8101 ctgctcccga accttttctc aatcaaaaga acacgacata caccgcagcc gccacaataa
     8161 agcgataaat ggcgaacggg ataaacgaaa tgcgcttaat caattgcagg aaggttttaa
     8221 tcgctatcag cgccaccaca aaagcggtga taaacccaac ggcaaacatc gggatatcgc
     8281 cggttgtcag gaagccccag cttttgtaga gatcgagcgc cgttgcgccc atcatcatcg
     8341 gtaccgccag caggaacgag aactcggaag cagcgtaacg gctcacgccc atcagcatcc
     8401 cacctgaaat ggtcgccccg gaacgggaga aacccggcca cagcgccaga cactggaaac
     8461 agccaatcat aaacgcctga cgataggtca tatcatcaag acccggcgca cgcggctctt
     8521 tcggcttcag gcattcggcg gcaatcagca acaaaccgcc aacgaccagc gcatacatca
     8581 catttatcgg gttaaacaat gacttaatcg tgtcgtggaa taatagcccc agtaccaccg
     8641 ccggaatcat ccccagcaaa atgtggatca gcgttaaacg acctttgctt tcaccttcgt
     8701 gctgcaacgg gcggccaaag tggatgccaa tcaggccaaa cagacgccgc cagaacatca
     8761 ctactaccgc cagaattgat cctaactgga tcacaacttc aaaggttttc gccgtgtcgc
     8821 cctcaaaccc caacaagtga ccgacaataa tcatatggcc cgtgctggat accggcagaa
     8881 attctgtcaa tccttcgacc acacccaata ttgccgctat cagcagcgag tgcatatcgc
     8941 tcatcaataa acccctaaat tattaaaatg taccgcttgt ccgaactact gcagtatgac
     9001 caggttataa ccgtttggtt taacagctgt aaaattaatt attttctttc agattattgc
     9061 cacgctcaat gattacgcca acattcgccg cccgcgccac tgcgcctggc ttgctgagtt
     9121 tgatacgcac ccacggcgag ttgaagcgtg ctaacagcag ctccgccacc tcttcagcca
     9181 cgcgttccac cagcgcaaaa cgcgccccct cgacgtggct gaccaccgtt tctgcaatgt
     9241 cagcgtaact gaggcaatcc gccacatcat cacttttcgc cgctttacgg ttatcccatg
     9301 ccatttcgat atcgaacact aacttctgtt cgatggtctg ttcccagtcg taaacaccaa
     9361 tagtggtgat taccgaaagt tgctctataa atacaatatc catcacgtcc tgcctgcttt
     9421 ttggctaacc ggataccact tccggcgaaa tgtgcgtatt atccacagat tcatcgttga
     9481 acacgaattt tcaaaacgga acagcttatg agtgcaatcg cgcctggaat gatcctcatc
     9541 gcgtacctct gcggctccat ttccagtgcc attctggttt gccgcttgtg tgggctgccc
     9601 gatccgcgaa ccagcggctc cggcaatcca ggcgcaacca atgtgttacg tatcggtggc
     9661 aagggagcag ccgtagcagt actgattttc gacgttctga aaggaatgtt gcctgtctgg
     9721 ggcgcgtatg aattaggtgt cagccccttc tggctaggct taattgccat cgccgcctgt
     9781 cttggacaca tctggcccgt tttcttcggt tttaaaggag gaaaaggcgt tgctaccgct
     9841 tttggagcaa tcgcaccaat tagctgggat ctcaccggag taatggcggg aacctggtta
     9901 ctgaccgtgc tattgagcgg atactcgtcg ctgggagcga ttgtcagtgc actgattgct
     9961 ccgttttatg tctggtggtt taagccacaa ttcaccttcc cggtttcgat gctctcttgc
    10021 ctgatcctgc tgcgtcatca tgacaacatc cagcgtctgt ggcgtcgtca ggagacaaaa
    10081 atctggacga aattcaaaag aaagcgcgaa aaggatcccg agtgatttct ggtggatcta
    10141 catgacctga tagccttcat cgggcttgcc cagccgttgc tggcaccatg ccgccagaaa
    10201 ttccgcgcag acacgaattt catgctgcga tagagcagct cccggtaaac agcccagata
    10261 ttggcgcttt gtgcatactc cggcaatact tgcaccagtt tgccactctc cagaaacggc
    10321 aacacatccc actcggaacg cagcataatc cctttgccct ccagcgccca ttgcaacact
    10381 atctcgccgc tattggagga aagatgcccg ctaaccttca ccgatttttt ctcctgaccg
    10441 ttccccaact cccatattcc gtgggtcata tcgcgctctt tggtcaccag acagtcatga
    10501 cgacttaatt cctgtaatga ttgtggctgc ggatattttt gcagatactc aggtgctgcg
    10561 cataatattc ttttattttt cgttaacaga tgcgcaatat aataatcagg aatttcgtca
    10621 ttaatacgaa tatccagatc aatattatcc tgcactaaat caatttgccg atcgaacaat
    10681 tcaaaatgca cctgtaactc aggataattg cgcataagtt cggtaatggc tggcgcaata
    10741 tggctgcgcc caaaaccaaa actgcaacca atacgaatca tcccctccgg gcgcgttttg
    10801 atttgcgtga cgtcatcgac cagtcgctga tattgggtga ggatttccag cgcatgttcg
    10861 taacaacgtt ggccactttc agtcagcgcc acgccgcgag cggagcggtt cagcagcgtt
    10921 gttgccagcg tattttcaag gatttggatc cgcttggtga caaatgcagg cgtttgccca
    10981 agcgtcgccg ctgcagcact aaaactcccg ctgtggacga tttctaccag tacctgcaag
    11041 tctttggcta aaggccagct attcagcatc gttaattatc cgcagttgtg ataagcgcag
    11101 tgtatttcgc aaaacattgc cattccctgc tctggcgcaa tattcattct tattttcgcg
    11161 ttgattcacg aactgttaat tacatattaa cggtggcaaa aaatataaaa ccacattttt
    11221 agtgttagtt tgtggcggtg aatttttcca gacaaataca aaaactggag ttgccatgat
    11281 gagcgaaagt aataagcaac aggcagtgaa taagttgaca gagattgtcg ctaactttac
    11341 cgccatgatt tctacccgaa tgcctgatga cgtggtggat aaactaaaac agctaaagga
    11401 tgccgaaacg tcgtcgatgg ggaaaattat ctaccatacg atgttcgaca acatgcaaaa
    11461 agcgattgac ctgaatcgtc ctgcctgtca ggacaccggg gagattatgt tcttcgttaa
    11521 agtcggttcc cgcttcccac tgcttggcga gctgcaaagc atactcaaac aagccgtgga
    11581 agaggcaacc gtcaaagcgc cactacgtca caatgcggta gaaatttttg acgaagtaaa
    11641 caccggcaaa aataccggta gcggcgtacc gtgggtcacc tgggacatca tccccgacaa
    11701 tgacgatgcg gaaatcgaag tttacatggc aggcggcggc tgcacgctac ctggccgctc
    11761 gaaagtgtta atgccgtcag aaggctacga aggcgtggtg aaattcgtct tcgaaaatat
    11821 ctccaccctc gccgtaaacg cctgtccacc ggtactggtg ggcgtgggca tcgccacctc
    11881 ggtggaaacc gccgccgtac tctcgcgtaa agccattttg cgcccgattg gcagccgcca
    11941 tcccaatcca aaagcggcag aactggagct acgcctggaa gaaggactca accgtctggg
    12001 gattggtcca caagggctga ccggcaacag ttcagtgatg ggcgcatcgt cgcggcacgc
    12061 tgctggttca tgccgatctc acctttgaaa atctgtctca cacccggagc gcgttatgaa
    12121 aaagatcctg acaaccccga tcaaagctga agatctgcaa gatattcgcg tcggcgatgt
    12181 gatctacctg accggtacgc tggtgacctg ccgcgacgtt tgtcaccgcc gtttgatcga
    12241 actgaaacgt ccgatccctt acgatctcaa cggcaaagcg attttccacg ctggccccat
    12301 cgtgcgcaaa aacggcgaca aatgggagat ggtctccgtc ggcccgacaa ccagtatgcg
    12361 tatggaaagt tttgaacgtg aatttattga gcagaccggc gtaaaactgg tggttggcaa
    12421 aggtggtatg gggccgctga ccgaagaagg ctgccagaaa ttcaaggcgt tacatgtgat
    12481 tttcccagca ggctgcgcgg tgctggcggc aacccaggta gaagagattg aagaagtgca
    12541 ctggacagaa ctcgggatgc cggagtcact gtgggtctgc cgggtcaaag agttcggccc
    12601 gctgattgtc tctattgata cccacggcaa caacctgata gccgaaaaca aaaagctgtt
    12661 cgccgaacgc cgcgatccca tcgtggaaga gatctgcgag cacgtccatt acatcaaata
    12721 accctcccgg agaggctcac ccctctcctt tttcgcaggc ataacacgat gaaaccttcc
    12781 actgaatggt ggcgatatct tgcgccgctg gcggtcatcg ccattattgc tctacttccg
    12841 gttcccgcag ggctggagaa ccatacctgg ctctactttg ccgtttttac tggcgtgatc
    12901 gttggactga tcctcgaacc cgtgccgggt gccgtggtgg cgatggtggg tatctccatc
    12961 atcgccatac tctctccctg gctgctgttc agccccgaac agctcgccca gccaggcttt
    13021 aagttcaccg ccaaatccct ctcctgggca gtttctggtt tttctaattc ggttatctgg
    13081 ctcattttcg ccgcctttat gtttggcaca ggctatgaaa aaaccgggct tggacgccgc
    13141 attgcgctga ttctggtgaa aaagatgggg catcgcacgc tgtttctcgg ctatgcggtg
    13201 atgttctccg agctgatcct ggcacctgta acaccgtcca actcggcgcg tggtgcgggt
    13261 attatctatc ccatcatccg taacctgcca ccgctctatc aatcacaacc aaacgacagc
    13321 agttcgcgca gcattggctc gtacatcatg tggatgggga ttgttgccga ctgcgtgacc
    13381 agcgccattt tcctgacggc gatggcacct aacttgctgt taattggact gatgaaaagc
    13441 gcatctcacg ccacgctgag ttggggcgac tggttcctcg ggatgttgcc gctcagcatt
    13501 ttactggttc tgctggttcc ctggctggct tacgtgctgt acccgccggt actgaagtct
    13561 ggtgatcagg tgccgcgctg ggcagagacg gaactgcagg caatgggccc gctctgttcg
    13621 cgtgaaaaac ggatgctggg gctgatggta ggcgcgctgg tgctgtggat tttcggcggt
    13681 gattatatcg atgccgcgat ggtcggttac agcgtggtgg cgctgatgct gcttctgcgc
    13741 attatcagtt gggacgacat tgtcagtaat aaagcggcat ggaacgtttt cttctggctg
    13801 gcctcgctta tcaccctcgc gaccggactc aacaataccg gttttattag ctggtttggc
    13861 aaactgttag caggcagctt aagtggttat tcgccgacga tggtgatggt gacgttgatt
    13921 gtggtgtttt atctactgcg ctactttttc gccagcgcca cggcgtatac ctgcgccctc
    13981 gcaccgatga tgatcgccgc cgcgctggcg atgccggaaa tcccgctgcc ggtgttctgc
    14041 ctgatggttg gcgcggcaat tggtctgggg agcattctta caccatacgc caccggaccc
    14101 agtccgattt actacggtag tggttatctg ccaacggcgg attactggcg actgggggcg
    14161 atttttgggc tgatattcct cgtgttgctg gtgattaccg gcttactgtg gatgcccgtg
    14221 gtgttgcttt aagtgaggac ggtgccggaa attgctctcc ggcacccaat agttttacgc
    14281 agccggtaac tccgccagcg gccagcgcgg acgcacgcta acgccgagat ccgccgtcgc
    14341 gcctgcttta aaccgcacca ttccggcata ggcgatcatc gcgccgttat cagtacaaaa
    14401 ttccggacgc gcgtagaaca cttcgccgcg gcgttttttc atcatttcag ccagcttcgc
    14461 ccgtaacgtg cggttagcac tcacgccgcc cgccatgacc agtcgcttaa agcccgtctg
    14521 atccaacgct cgcttacact taatcatcaa cgtatcgacc accgcatctt caaaggcgcg
    14581 ggcgatatca gcacgcgtct ggtcgtcggt gccgttgtca cgaatcgtgt tcgccgcaaa
    14641 ggttttcaga ccagaaaagc tgaaatccag ccccggacgg tcggtcatcg gacgcgggaa
    14701 aacaaagcgc ccggcagtac cctgagccgc cattttcgac agtaacggtc cgccaggata
    14761 atccagcccc agcagcttcg cggttttatc aaacgcttca ccggcggcat catcgataga
    14821 ctcgccgagc agctcgtact gaccaatgcc agtcacgctg attaactgcg tatgaccgcc
    14881 ggacaccagc agcgcgacaa acggaaattc cggcgggtta tcttccagca tcggcgctaa
    14941 cagatgccct tccatatggt gtaccgggat tgccggaacg ttccaggcaa acgccagaga
    15001 acgccccacg gttgcgccaa ccagcagtgc gccgactaat ccagggcctg cggtataggc
    15061 cacagcatca atgtcttttg ccgttaaacc agactccttt agcgccgcct ggatcaacgg
    15121 tacggtttta cgcacatgat cgcgggaggc cagttcaggc acgacgccgc cgtagtcagc
    15181 gtgcaatttc acctgactat acaattggtt ggctaacaaa cctttttcat cgtcgtaaat
    15241 ggcgatgccg gtttcatcgc aggaagtttc aatacccagt acacgcatga cttgttttac
    15301 ctcgctttat taccgcgcag tgtaggacca atgcgggttg atgtaaaact ttgttcgccc
    15361 ctggagaaag cctcgtgtat actcctcacc cttataaaag tccctttcaa aaaaggccgc
    15421 ggtgctttac aaagcagcag caattgcagt aaaattccgc accattttga aataagctgg
    15481 cgttgatgcc agcggcaaac cgaattaatc aaaggtgaga ggcacatgcc ggtaattaaa
    15541 gtacgtgaaa acgagccgtt cgacgtagct ctgcgtcgct tcaagcgttc ctgcgaaaaa
    15601 gcaggtgttc tggcggaagt tcgtcgtcgt gagttctatg aaaaaccgac taccgaacgt
    15661 aagcgcgcta aagcttctgc agtgaaacgt cacgcgaaga aactggctcg cgaaaacgca
    15721 cgccgcactc gtctgtacta attccccgag agcgttgctc tccgatcaga ccgagttgta
    15781 gttgtaaggc cgtgcttccg aaaggaatgc gcggcttatt ttcgtttatg aattgctaaa
    15841 aatcggggcc tatggctgga cgaatcccac gcgtattcat taatgatctg ctggcacgca
    15901 ctgacatcgt cgatctgatc gatgcccgtg tgaagctgaa aaagcagggc aagaatttcc
    15961 acgcgtgttg tccattccac aacgagaaaa ccccgtcatt caccgttaac ggtgagaaac
    16021 agttttacca ctgctttgga tgtggcgcgc acggcaacgc gatcgacttc ctgatgaact
    16081 acgacaagct cgagttcgtc gaaacggtcg aagagctggc agcaatgcac aatcttgaag
    16141 tgccatttga agcaggcagc ggccccagcc agatcgagcg ccatcagagg caaacgcttt
    16201 atcagttgat ggacggtctg aatacgtttt accaacaatc tttacaacaa cctgttgcca
    16261 cgtctgcgcg ccagtatctg gaaaaacgcg gattaagcca cgaggttatc gctcgctttg
    16321 cgattggttt tgcgcccccc ggctgggaca acgtcctgaa gcggtttggc ggcaatccag
    16381 aaaatcgcca gtcattgatt gatgcgggga tgttggtcac taacgatcag ggacgcagtt
    16441 acgatcgttt ccgcgaacgg gtgatgttcc ccattcgcga taaacgcggt cgggtgattg
    16501 gttttggcgg gcgcgtgctg ggcaacgata cccccaaata cctgaactcg ccggaaacag
    16561 acattttcca taaaggccgc cagctttacg gtctttatga agcgcagcag gataacgctg
    16621 aacccaatcg tctgcttgtg gtcgaaggct atatggacgt ggtggcgctg gcgcaatacg
    16681 gcattaatta cgccgttgcg tcgttaggta cgtcaaccac cgccgatcac atacaactgt
    16741 tgttccgcgc gaccaacaat gtcatttgct gttatgacgg cgaccgtgca ggccgcgatg
    16801 ccgcatggcg agcgctggaa acggcgctgc cttacatgac agacggccgt cagctacgct
    16861 ttatgttttt gccggatggc gaagaccctg acacgctggt acgaaaagaa ggtaaagaag
    16921 cgtttgaagc gcggatggag caggcgatgc cactctccgc atttctgttt aacagtctga
    16981 tgccgcaagt tgatctgagt acccctgacg ggcgcgcacg tttgagtacg ctggcactgc
    17041 cattgatatc gcaagtgccg ggcgaaacgc tgcgaatata tcttcgtcag gaattaggca
    17101 acaaattagg catacttgat gacagccagc ttgaacgatt aatgccaaaa gcggcagaga
    17161 gcggcgtttc tcgccctgtt ccgcagctaa aacgcacgac catgcgtata cttatagggt
    17221 tgctggtgca aaatccagaa ttagcgacgt tggtcccgcc gcttgagaat ctggatgaaa
    17281 ataagctccc tggacttggc ttattcagag aactggtcaa cacttgtctc tcccagccag
    17341 gtctgaccac cgggcaactt ttagagcact atcgtggtac aaataatgct gccacccttg
    17401 aaaaactgtc gatgtgggac gatatagcag ataagaatat tgctgagcaa accttcaccg
    17461 actcactcaa ccatatgttt gattcgctgc ttgaactgcg ccaggaagag ttaatcgctc
    17521 gtgagcgcac gcatggttta agcaacgaag aacgcctgga gctctggaca ttaaaccagg
    17581 agctggcgaa aaagtgattt aacggcttaa gtgccgaaga gcgatcggga agcccccgac
    17641 agccgcactg agaggcagcg gcaaatatat aagtacgccc tcgtaattat cgttggcggt
    17701 aaacaaccgt tggatttcag cgttaacggc tgaaggacat cgggtcaatc gcccaacacc
    17761 aacctcatga aataagtgtg gataccgtct tatggagcaa aacccgcagt cacagctgaa
    17821 acttcttgtc acccgtggta aggagcaagg ctatctgacc tatgccgagg tcaatgacca
    17881 tctgccggaa gatatcgtcg attcagatca gatcgaagac atcatccaaa tgatcaacga
    17941 catgggcatt caggtgatgg aagaagcacc ggatgccgat gatctgatgc tggctgaaaa
    18001 caccgcggac gaagatgctg ccgaagccgc cgcgcaggtg ctttccagcg tggaatctga
    18061 aatcgggcgc acgactgacc cggtacgcat gtacatgcgt gaaatgggca ccgttgaact
    18121 gttgacccgc gaaggcgaaa ttgacatcgc taagcgtatt gaagacggga tcaaccaggt
    18181 tcaatgctcc gttgctgaat atccggaagc gatcacctat ctgctggaac agtacgatcg
    18241 tgttgaagca gaagaagcgc gtctgtccga tctgatcacc ggctttgttg acccgaacgc
    18301 agaagaagat ctggcaccta ccgccactca cgtcggttct gagctttccc aggaagatct
    18361 ggacgatgac gaagatgaag acgaagaaga tggcgatgac gacagcgccg atgatgacaa
    18421 cagcatcgac ccggaactgg ctcgcgaaaa atttgcggaa ctacgcgctc agtacgttgt
    18481 aacgcgtgac accatcaaag cgaaaggtcg cagtcacgct gccgctcagg aagagatcct
    18541 gaaactgtct gaagtattca aacagttccg cctggtgccg aagcagtttg actacctggt
    18601 caacagcatg cgcgtcatga tggaccgcgt tcgtacgcaa gaacgtctga tcatgaagct
    18661 ctgcgttgag cagtgcaaaa tgccgaagaa aaacttcatt accctgttta ccggcaacga
    18721 aaccagcgat acctggttca acgcggcaat tgcgatgaac aagccgtggt cggaaaaact
    18781 gcacgatgtc tctgaagaag tgcaccgcgc cctgcaaaaa ctgcagcaga ttgaagaaga
    18841 aaccggcctg accatcgagc aggttaaaga tatcaaccgt cgtatgtcca tcggtgaagc
    18901 gaaagcccgc cgtgcgaaga aagagatggt tgaagcgaac ttacgtctgg ttatttctat
    18961 cgccaagaaa tacaccaacc gtggcttgca gttccttgac ctgattcagg aaggtaacat
    19021 cggcctgatg aaagcggttg ataaattcga ataccgccgt ggttacaagt tctccaccta
    19081 cgcaacctgg tggatccgtc aggcgatcac ccgttctatc gcggatcagg cgcgcaccat
    19141 ccgtattccg gtgcatatga ttgagactat caacaaactc aaccgtattt ctcgccagat
    19201 gctgcaagag atgggccgtg aaccgacgcc ggaagaactg gctgaacgta tgctgatgcc
    19261 ggaagacaag atccgcaaag tgctgaagat cgccaaagag ccaatctcca tggaaacgcc
    19321 gatcggtgat gatgaagatt cgcatctggg ggatttcatc gaggatacca ccctcgagct
    19381 gccgctggat tctgcgacca ccgaaagcct gcgtgcggca acgcacgacg tgctggctgg
    19441 cctgaccgcg cgtgaagcaa aagttctgcg tatgcgtttc ggtatcgata tgaacaccga
    19501 ccacacgctg gaagaagtgg gtaaacagtt cgacgttacc cgcgaacgta tccgtcagat
    19561 cgaagcgaag gcgctgcgca aactgcgtca cccgagccgt tctgaagtgt tgcgtagctt
    19621 cctggacgat taatcggtag gccggatcag gcgttacgcc gcacccggca ctagtctctc
    19681 tgcacaaacg ccaccttt
//
LOCUS       DAZPCS010000065        19365 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZPCS010000065 DAZPCS010000000
VERSION     DAZPCS010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19365
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.065"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..122
                     /locus_tag="ACHUQ7_002218"
     CDS             <1..122
                     /locus_tag="ACHUQ7_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFR1135617.1"
                     /translation="ADAKKVQRQILHVPGFWAELSWHLRQRTTGKTKALYNKV"
     gene            138..686
                     /gene="wcaF"
                     /locus_tag="ACHUQ7_002219"
     CDS             138..686
                     /gene="wcaF"
                     /locus_tag="ACHUQ7_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFR1135618.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            712..1833
                     /gene="gmd"
                     /locus_tag="ACHUQ7_002220"
     CDS             712..1833
                     /gene="gmd"
                     /locus_tag="ACHUQ7_002220"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFR1135619.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            1836..2804
                     /gene="fcl"
                     /locus_tag="ACHUQ7_002221"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             1836..2804
                     /gene="fcl"
                     /locus_tag="ACHUQ7_002221"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFR1135620.1"
                     /translation="MSKQRIFIAGHCGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     MFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRT
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRGE"
     gene            2804..3283
                     /gene="gmm"
                     /locus_tag="ACHUQ7_002222"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             2804..3283
                     /gene="gmm"
                     /locus_tag="ACHUQ7_002222"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFR1135621.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            3280..4503
                     /gene="wcaI"
                     /locus_tag="ACHUQ7_002223"
     CDS             3280..4503
                     /gene="wcaI"
                     /locus_tag="ACHUQ7_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFR1135622.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            4506..5942
                     /gene="cpsB"
                     /locus_tag="ACHUQ7_002224"
     CDS             4506..5942
                     /gene="cpsB"
                     /locus_tag="ACHUQ7_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFR1135623.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSVGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMEPTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            6135..7505
                     /gene="cpsG"
                     /locus_tag="ACHUQ7_002225"
                     /gene_synonym="manB"
     CDS             6135..7505
                     /gene="cpsG"
                     /locus_tag="ACHUQ7_002225"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFR1135624.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARTISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            7560..8954
                     /gene="wcaJ"
                     /locus_tag="ACHUQ7_002226"
     CDS             7560..8954
                     /gene="wcaJ"
                     /locus_tag="ACHUQ7_002226"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HFR1135625.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            8956..10434
                     /gene="wzxC"
                     /locus_tag="ACHUQ7_002227"
     CDS             8956..10434
                     /gene="wzxC"
                     /locus_tag="ACHUQ7_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HFR1135626.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            10614..11894
                     /gene="wcaK"
                     /locus_tag="ACHUQ7_002228"
     CDS             10614..11894
                     /gene="wcaK"
                     /locus_tag="ACHUQ7_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HFR1135627.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRIKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRRNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPVLNARFNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            11891..13111
                     /gene="wcaL"
                     /locus_tag="ACHUQ7_002229"
     CDS             11891..13111
                     /gene="wcaL"
                     /locus_tag="ACHUQ7_002229"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HFR1135628.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTIIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLLPENDARALAQRLAAFSQLDTDELAPVVKRACEKVEHDFNQ
                     QVINRELASLLQAL"
     gene            13122..14516
                     /gene="wcaM"
                     /locus_tag="ACHUQ7_002230"
     CDS             13122..14516
                     /gene="wcaM"
                     /locus_tag="ACHUQ7_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HFR1135629.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRGSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            14691..15584
                     /gene="galF"
                     /locus_tag="ACHUQ7_002231"
     CDS             14691..15584
                     /gene="galF"
                     /locus_tag="ACHUQ7_002231"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HFR1135630.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVIIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            15957..17042
                     /gene="rfbB"
                     /locus_tag="ACHUQ7_002232"
     CDS             15957..17042
                     /gene="rfbB"
                     /locus_tag="ACHUQ7_002232"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFR1135631.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGTYQSWIEQNYEGRQ"
     gene            17042..17941
                     /gene="rfbD"
                     /locus_tag="ACHUQ7_002233"
     CDS             17042..17941
                     /gene="rfbD"
                     /locus_tag="ACHUQ7_002233"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFR1135632.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            18000..18878
                     /gene="rfbA"
                     /locus_tag="ACHUQ7_002234"
     CDS             18000..18878
                     /gene="rfbA"
                     /locus_tag="ACHUQ7_002234"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFR1135633.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPQEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAHRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            18883..>19365
                     /gene="rfbC"
                     /locus_tag="ACHUQ7_002235"
     CDS             18883..>19365
                     /gene="rfbC"
                     /locus_tag="ACHUQ7_002235"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFR1135634.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
                     N"
BASE COUNT         4821 a         4686 c         5279 g         4579 t
ORIGIN      
        1 gtgctgacgc caaaaaagtc caacggcaga tattacatgt acctggcttc tgggcagaat
       61 tatcctggca tttacgccaa cgtacgacag gtaagactaa agccttatat aacaaagtct
      121 gaatataagg aaaaccaatg caagatttaa gcggtttctc ggtgccgaaa gggttccggg
      181 gcggcaacgc tattaaagtg caattatggt gggcagtaca ggcaacaata tttgcctggt
      241 cgccacaagt attgtatcgc tggcgggctt ttttattacg tttattcgga gcaaaaatag
      301 gaaaaaacgt agttattcgt ccgtcagtaa aaattaccta tccgtggaaa ttaaccttag
      361 gtgattacgc gtgggttggc gatgacgtca atttatatac cctcggtgaa ataaccattg
      421 gcgcacattc ggtgatatcg caaaaaagtt atttatgcac cggtagccac gaccatgcaa
      481 gtcaacattt caccattaac gccacgccta ttgtgattgg cgagaaatgc tggctggcaa
      541 ccgatgtctt tgttgcccca ggcgtcacaa tcggcgacgg caccgtcgtg ggtgcacgaa
      601 gcagtgtttt taaatcgctt ccggcaaatg tggtttgccg ggggaatccc gcagtggtga
      661 tacgcgaacg cgttgaaact gaataaattc aaaaatacag aggaataaga catgtcaaaa
      721 gtcgctctca tcaccggtgt aaccggacaa gacggttctt acctggcaga gtttctgctg
      781 gaaaaaggtt acgaggtgca tggtattaag cgtcgcgcat cgtcattcaa caccgagcgc
      841 gtggatcaca tttatcagga tccgcacacc tgcaacccga aattccatct gcattatggc
      901 gacctgagtg atacctctaa cctgacgcgc attttgcgtg aagtacagcc ggatgaagtg
      961 tacaacctag gcgcaatgag ccacgttgcg gtctcttttg agtcaccaga atataccgct
     1021 gacgtcgacg cgatgggtac gctgcgcctg ctggaggcga tccgcttcct cggtctggaa
     1081 aagaaaactc gtttctatca ggcttccacc tctgaactgt atggtctggt gcaggaaatt
     1141 ccgcagaaag aaaccacgcc gttctacccg cgatctccgt atgcggtcgc caaactgtac
     1201 gcctactgga tcaccgttaa ctaccgcgaa tcctacggca tgtacgcttg taacggaatt
     1261 ctcttcaacc atgaatcccc gcgccgcggc gaaaccttcg ttacccgcaa aatcacccgc
     1321 gcaatcgcca acatcgccca ggggctggag tcgtgcctgt acctcggcaa tatggattcc
     1381 ctgcgtgact ggggccacgc caaagactac gtaaaaatgc agtggatgat gctgcaacag
     1441 gaacagccgg aagatttcgt tatcgcgacc ggcgttcagt actccgtacg tcagtttgtg
     1501 gaaatggcgg cagcacagct gggcatcaaa ctgcgctttg aaggcacggg tgttgaagag
     1561 aagggcattg tggtttccgt caccgggcat gacgcgccgg gcgttaaacc gggtgatgtg
     1621 attatcgccg ttgatccgcg ttacttccgt ccggcagaag ttgaaacgct gctcggcgac
     1681 ccgaccaaag cgcacgaaaa actgggctgg aaaccggaaa tcaccctcag agagatggtg
     1741 tctgaaatgg tggctaatga cctcgaagcg gcgaaaaaac actctctgct gaaatctcac
     1801 ggctacgacg tggcgatcgc gctggagtca taagcatgag taaacaacgc atttttatcg
     1861 ctggccattg tgggatggtc ggttctgcca tcaagcggca gctcgaacag cgcggtgatg
     1921 tggagctggt attacgtacc cgcgatgaac tgaacctgct ggacagccgc gccgtgcatg
     1981 atttctttgc cagcgaacgc attgaccagg tctatctggc ggcggcgaaa gtgggcggca
     2041 ttgttgccaa caacacctat ccggcggatt tcatctacca gaacatgatg attgagagca
     2101 acatcattca cgccgcgcat cagaacgacg tgaacaaact gatgtttctc ggatcgtcct
     2161 gcatctaccc gaaactggca aaacagccga tggcagaaag cgagttattg cagggcacgc
     2221 tggagccgac caacgagcct tacgccattg ccaaaatcgc cgggatcaaa ctgtgcgaat
     2281 cttacaaccg ccagtatgga cgcgattacc gcactgtcat gccgaccaac ctgtacgggc
     2341 cgcatgacaa cttccacccg agtaactccc atgtgatacc agcattgctg cgtcgcttcc
     2401 acgaggcgac ggcacagaat gcgccggacg tggtggtatg gggcagcggt acaccgatgc
     2461 gtgaattcct gcacgtcgat gatatggcgg cggcgagcat tcatgtcatg gagctggcgc
     2521 atgaagtctg gctggagaat acccaaccga tgctgtcgca cattaacgtc ggcacgggcg
     2581 ttgactgcac tatccgcgag ctggcgcaaa ccatcgccaa agtggtgggt tacaaaggtc
     2641 gggtggtttt tgatgccagc aaaccggatg gtacgccgcg caaactgctg gatgtgacgc
     2701 gcctgcatca gcttggctgg tatcacgaaa tctcactgga agcggggctt gccagcactt
     2761 accagtggtt ccttgagaat caagaccgct ttcgggggga atgatgtttt tacgtcagga
     2821 agactttgcc actgtagtgc gctccacacc gcttgtctct ctcgacttta ttgtcgagaa
     2881 cagtcgcggc gagtttctgc ttggcaaaag aaccaaccgc ccggcgcagg gttactggtt
     2941 tgtgccggga gggcgcgtgc agaaagacga aacgctggaa gccgcatttg agcggctgac
     3001 gatggcggaa ctggggctgc gtctgccgat aacagcaggc cagttttacg gtgtctggca
     3061 gcatttttat gacgataact tctccggcac ggatttcacc actcactatg tggtgctcgg
     3121 ttttcgcttc agagtagcgg aagaagagct gttactgccg gatgagcagc atgacgatta
     3181 ccgctggctg acgccggacg cgctgctcgc cagtaacgat gttcacgcta acagccgagc
     3241 ctattttctc gctgagaagc gtgccggagt acccggatta tgaaaatact ggtctacggc
     3301 attaactact cgccggagtt aaccggcatc ggcaaataca ccggcgagat ggtggaatgg
     3361 ttggcggcac aaggtcatga ggtgcgggtc attaccgcac cgccttacta cccgcaatgg
     3421 caggtgggtg agaactattc cgcctggcgc tacaaacgag aagaaggggc cgccacggtg
     3481 tggcgctgcc cgctgtacgt gccaaaacag ccgagcaccc tgaaacgctt gttgcatctc
     3541 ggcagttttg ccgtcagcag tttctttcca ctgatggcgc aacgtcgctg gaagccggat
     3601 cgcattattg gcgtagtgcc aacgctgttt tgcacgccgg gaatgcgcct gctggcgaag
     3661 ctctctggtg cgcgtaccgt gctgcatatt caggattacg aagtggacgc catgctgggg
     3721 ctgggccttg ccggaaaagg caaaggcggc aaagtggcac agctggcaac ggcgttcgaa
     3781 cgtagcggac tgcataacgt cgataacgtt tccacgattt cgcgttcgat gatgaataaa
     3841 gccatcgaaa aaggcgtggc ggcggataac gtcatcttct tccccaactg gtcggaaatt
     3901 gcccgttttc agcatgttgc agacgccgat gttgatgccc ttcgtaacca gcttggcctg
     3961 ccggataaca aaaaaatcat tctttactcc ggcaatattg gtgaaaagca ggggctggaa
     4021 aacgttattg aagctgccga tctcctgcgc gatgaaccgc tgatttttgc cattgtcggg
     4081 cagggcggcg gcaaagcgcg gctggaaaaa atggcgcagc agcgtggact gcgcaacatg
     4141 caatttttcc cgctgcaatc gtatgacgct ttacctgcac tgctgaagat gggcgattgc
     4201 catctggtgg tgcaaaaacg cggcgcggca gatgccgtat tgccgtcgaa actgaccaat
     4261 attctggcgg taggcggtaa cgcggtgatt accgcagagg cccacacaga actggggcag
     4321 ctttgcgaaa cctttccggg tattgcggtt tgcgtagaac cggaatcggt cgaggcgctg
     4381 gtggcgggga ttcgtcaggc gctcctgctg cccaaacata acacggtggc acgtgaatat
     4441 gccgaacgca cgctcgataa agagaacgtg ttacgtcaat ttataaatga tattcgggga
     4501 taattatggc gcagtcgaaa ctctatccag ttgtgatggc aggtggctcc ggtagccgtt
     4561 tatggccgct ttcccgcgta ctttacccca agcagttttt atgcctgaaa ggcgatctca
     4621 ccatgctgca aaccaccatc tgccgcctga acggcgtgga gtgcgaaagc ccggtggtga
     4681 tttgcaatga gcagcaccgc tttattgtcg cggaacagct gcgtcaactg aacaaactca
     4741 ccgagaacat tattctcgaa ccggcagggc gtaacactgc acctgccatt gcactggcgg
     4801 cgctggcggc aaaacgtcat agcccggaga gcgacccgtt aatgctggta ttggcggcgg
     4861 atcatgtgat tgccgatgaa gacgcgttcc gtgccgccgt gcgtaatgcc atgccttatg
     4921 ccgaagcggg caagctggtg acctttggca ttgtgccgga tctaccagaa accggttatg
     4981 gctatattcg tcgcggtgaa gtgtctgtgg gtgagcagga tacggtggcc tttgaagtgg
     5041 cgcagtttgt cgaaaaaccg aatctggaaa ccgcccaggc ctatgtagca agcggcgaat
     5101 attactggaa cagcggcatg ttcctgttcc gcgccggacg ctatctcgaa gaactgaaaa
     5161 aatatcgccc ggatatcctc gatgcctgcg aaaaagcgat gagcgccgtc gatccggatc
     5221 tcgattttat tcgtgtggat gaagaagcgt ttctcgcctg cccggaagag tcggtggatt
     5281 acgcggtcat ggaacctacg gcagatgctg ttgtggtgcc gatggatgcg ggctggagcg
     5341 atgttggttc ctggtcttca ttatgggaga tcagcgccca caccgccgag ggcaacgttt
     5401 gccacggcga tgtgattaat cacaaaactg aaaacagcta tgtgtatgcc gaatcaggcc
     5461 tggtcaccac cgtcggggtg aaagatctgg tagtggtgca gaccaaagat gcggtgctga
     5521 ttgccgaccg taacgcggta caggatgtga aaaaagtggt cgagcagatc aaagccgatg
     5581 gtcgccatga gcatcgggtg catcgcgaag tgtatcgtcc gtggggcaaa tatgactcta
     5641 tcgacgcggg cgaccgctac caggtgaaac gcatcaccgt gaaaccgggc gagggtttgt
     5701 cggtacagat gcaccatcac cgcgcggaac actgggtagt ggtcgcggga acggcaaaag
     5761 tcaccattga tggtgatatc aaactgcttg gtgaaaacga gtccatttat attccgctgg
     5821 gggcgacgca ctgcctggaa aacccgggga aaattccgct cgatttaatt gaagtgcgct
     5881 ccggctctta tctcgaagag gatgatgtgg tgcgcttcgc ggatcgctac ggacgggtgt
     5941 aaacgtcgca tcaggcgctg tttgtcggat gcggcgtgaa cgccttatcc gacctacggt
     6001 tcggttttgt aggcctgata agacgcggca gcgtcgcatc aggcaatgaa tgcgaaaccg
     6061 cggtgtaaat aacgacaaaa ataaaattgg ccgtttcggt cagggccaac tattgcctga
     6121 aaaagggtaa cgacatgaaa aaattaacct gctttaaagc ctatgatatt cgcgggaaat
     6181 taggcgaaga actgaatgaa gatatcgcct ggcgcattgg tcgcgcctat ggcgaatttc
     6241 tcaaaccgaa aaccattgtg ttaggcggtg atgtccgtct caccagcgaa accttaaaac
     6301 tggcgctggc aaaaggttta caggatgcgg gcgtcgatgt gctggatatc ggtatgtccg
     6361 gcaccgaaga gatctatttc gccacgttcc atctcggcgt ggatggcggc attgaagtta
     6421 ctgccagcca taatccgatg gattacaacg gcatgaagct ggtgcgcgag ggggctcgca
     6481 cgatcagcgg tgataccgga ctgcgcgacg tccagcgtct ggctgaagcc aacgactttc
     6541 ctcccgtcga tgaaaccaaa cgcggtcgct atcagcaaat caacctgcgt gacgcttacg
     6601 ttgatcacct gttcggttat atcaatgtca aaaatctcac gccgctcaag ctggtgatca
     6661 actccgggaa tggcgcagcg ggtccggtgg tggacgccat tgaagcccgc tttaaagccc
     6721 tcggcgcgcc cgtggaatta atcaaagtac acaacacacc ggacggcaat ttccccaacg
     6781 gtattcctaa cccgctgctg ccggaatgtc gcgacgacac ccgcaatgcg gtcatcaaac
     6841 acggcgcgga tatgggcatt gcctttgatg gcgattttga ccgctgtttt ctgtttgacg
     6901 aaaaagggca gtttatcgag ggctactaca ttgtcggcct gctggcagaa gcgttcctcg
     6961 aaaaaaatcc cggcgcgaag atcatccatg atccgcgtct ctcttggaac actattgatg
     7021 tggtgactgc tgcaggcggc acgccggtaa tgtcgaaaac cggacacgcc tttattaaag
     7081 aacggatgcg caaggaagac gccatctacg gtggcgaaat gagcgcccac cattacttcc
     7141 gtgatttcgc ttactgcgac agcggcatga tcccgtggct gctggtcgcc gaactggtgt
     7201 gcctgaaagg aaaaacgctg ggcgaactgg tgcgcgaccg gatggcggcg tttccggcaa
     7261 gcggtgagat caacagcaaa ctggcgcaac ccgttgaggc gattaaccgc gtggaacagc
     7321 attttagccg tgaggcgctg gcggtggatc gcagcgatgg catcagcatg acctttgccg
     7381 actggcgctt taacctgcgc acctccaata ccgaaccggt ggtgcgcctg aatgtggaat
     7441 cgcgcggtga tgtgccgctg atggaagcgc gaacgcgaac tctgctgacg ttgctgaacg
     7501 agtaatgtcg gatcttccct taccccactg cgggtaaggg gctaataaca ggaacaacga
     7561 tgacaaatct aaaaaagcgc gagcgagcga aaaccaatgc atcgttaatc tctatggtgc
     7621 aacgcttttc agatatcacc atcatgtttg ccgggctatg gctggtttgc gaagtcagcg
     7681 gactgtcatt cctctacatg cacctgttgg tggcgctgat tacgctggtg gtgttccaga
     7741 tgctgggcgg catcaccgat ttttatcgct catggcgcgg tgttcgggca gcgacagaat
     7801 ttgccctgtt gctacaaaac tggaccttaa gcgtgatttt cagcgccgga ctggtggcgt
     7861 tcaacaatga tttcgacacg caactgaaaa tctggctggc gtggtatggg ctgaccagca
     7921 tcggactggt ggtttgccgt tcatgtattc gcattggggc gggctggctg cgtaatcatg
     7981 gctacaacaa gcggatggtc gccgtagcgg gggatttagc cgccggacaa atgctgatgg
     8041 agagtttccg taatcagccg tggttagggt ttgaagtggt gggcgtttac cacgacccga
     8101 aaccgggcgg cgtttctaac gactgggcgg gtaacctgca acagctggtc gaggatgcaa
     8161 aagcgggcaa gattcataac gtctatatcg cgatgcaaat gtgcgacggc gcacgagtga
     8221 aaaaactggt ccatcaactg gcggatacca cctgttcggt gctgctgatc cccgacgtct
     8281 ttaccttcaa cattctccat tcacgtctcg aagagatgaa cggcgttccg gtggtgccgc
     8341 tgtatgacac gccgctttcg ggggttaacc gcctgctaaa acgtgcggaa gacattgtgc
     8401 tggcgacgct tattctgctg ctgatctccc cggtgctgtg ctgtattgcg ctggcggtga
     8461 aactcagttc accagggccg gttattttcc gccagactcg ctatggtatg gatggcaagc
     8521 cgatcaaagt gtggaagttc cgttccatga aagtgatgga gaacgacaaa gtggtgaccc
     8581 aggcgacgca gaacgatccg cgcgtcacta aagtggggaa ctttctgcgc cgcacctcgc
     8641 tggacgaatt gccgcagttt atcaatgtgc tgaccggggg gatgtcgatt gtcggtccac
     8701 gtccgcacgc agtagcgcat aacgaacagt atcgccagct cattgaaggc tacatgctgc
     8761 gccataaagt gaaaccgggc attaccggct gggcgcagat taatggctgg cgcggcgaaa
     8821 ccgacacgct ggagaaaatg gaaaaacgcg tcgagttcga ccttgagtac atccgcgaat
     8881 ggagcgtctg gttcgatatc aaaatcgttt tcctgacggt attcaagggc ttcgttaaca
     8941 aagcggcata ttgatatgag cttacgtgaa aaaaccatca gcggcgcgaa gtggtcggct
     9001 attgccacgg tgatcatcat cggcctcggg ctggtgcaga tgaccgtgct ggcgcggatt
     9061 atcgacaacc accagttcgg cctgctgact gtgtcgctgg tgattatcgc gctggcagat
     9121 acgctttctg acttcggtat cgctaactcg attattcagc gaaaagaaat cagtcacctt
     9181 gaactcacca cgctgtactg gctgaacgtc gggctggggc ttgtggtatg tgtggcggtg
     9241 tttttgttga gtgatgtcat cggcgacgtg ctgaataacc cggacctggc accgttgatt
     9301 aaaacattgt cgctggcgtt tgtggtgatc ccccacgggc aacagttccg cgcgttgatg
     9361 cagaaggagc tggagttcaa caaaatcggc atgatcgaaa ccagcgcggt gctggcgggc
     9421 ttcactttta cggtagttag cgcccatttc tggccgctgg cgatgaccgc gatcctcggt
     9481 tatctggtca atagtgcggt gagaacactg ctgtttggct actttggacg caaaatttat
     9541 tgccccggtc tgcacttctc gctggcgtct gtggcaccga acttacgttt tggtgcctgg
     9601 ctgacggcgg acagcatcat caactatctc aacaccaacc tttcaacgct ggtgctggcg
     9661 cgtattcttg gcgcgggcgt ggcaggggga tacaacctgg cgtacaacgt ggccgtcgta
     9721 ccgccgatga agctgaaccc aatcatcacc cgcgtgttgt ttccggcgtt tgccaaaatt
     9781 caggacgaca ccgaaaagct gcgtgttaac ttctacaagc tgctgtcgat agtggggatt
     9841 atcaactttc cggcgctgct ggggctgatg gtggtgtcga ataacttcgt accgctggta
     9901 ttcggcgaaa agtggaacag cattattccg gtgctgcaat tgctgtgtat tgtgggtctg
     9961 ctgcgctccg tgggtaaccc gattggttcg ctgctgatgg cgaaagcgcg ggtcgatatc
    10021 agctttaaat tcaacgtatt caaaacattt ctgtttattc cggcgattgt tattggtggg
    10081 cagatggcgg gcgcaatcgg cgtcacgctc ggtttcctgc tggtgcagat tatcaacacc
    10141 attctgagtt acttcgtgat gattaaaccg gttcttggtt ccagttatcg ccagtacatc
    10201 ctgagtttgt ggctgccgtt ttatctctcg ttgccgacgc tggtggtcag ttatgcgctg
    10261 ggcattgtgc tgaaagggca actggcgctg gggatgctgc tggcggtgca aatagccgcg
    10321 ggcgtgctgg cgtttgtggt gatgattgtg ctgtcacgcc atccgctggt ggtggaagtg
    10381 aagcgtcagt tttgtcgcag cgaaaaaatg aaaatgcttt tacgggcggg gtgaattgct
    10441 gttttcggtt ccgacaacgg ttgccggatg cggcgtaaaa cgccttatcc gtcctacggt
    10501 tctgtgctcg ggacctgtag gcctgataag acgcgccagc gtcgcatcag gcaaaaaacg
    10561 gcaacgaaca caacaaacac agcctcacct ggcatcaaat ttatgaggaa aaaatgaaat
    10621 tacttattct gggcaaccac acttgcggca atcgtggcga cagcgccatc ttgcgcggct
    10681 tacttgatgc catcaacatc ctcaatccac ataccgaagt ggacgtgatg agccgctatc
    10741 cggtcagttc ttcctggctg ctcaaccgcc cggtaatggg cgatccgctg ttcctgcaga
    10801 tgacacaaca caatagcgcg gcgggcgttg tcgggcgcat taaaaaagtc ctccgtcgcc
    10861 gctaccagca tcaggtattg ctctcacgcg tcaccgacac tggcaagctg cgcaatatcg
    10921 ccatcgccca gggattcacc gacttcgtgc gcctgctgtc aggttacgac gccattattc
    10981 aggtcggcgg atcgtttttt gtcgatctct acggcgtgcc gcagtttgaa catgcgcttt
    11041 gcacatttat ggcgaaaaag ccgctgttta tgattggtca cagcgtcggc cccttccagg
    11101 atgagcaatt taaccaactg gcgaactatg tctttggtca ctgcgacgcg ctgatcctgc
    11161 gcgaatcggt cagccttgat ttgatgaaac gcagaaatat caccaccgca aaagtggaac
    11221 atggcgtcga taccgcgtgg ctggtcgatc accacaccga agacttcacc gccagctatg
    11281 ccgttcaaca ctggctggac gttgccgcac aacagaaaac agtggcaatt accctgcgcg
    11341 aactggcacc gttcgacaaa cgtctcggca ccactcaaca agcgtatgaa aaagcctttg
    11401 ccggggtggt caatcgcatt ctcgatgaag gctatcaggt gattgcgctc tccacctgta
    11461 cgggtatcga cagctataac aaagacgacc gcatggtggc gctcaatctg cgccagcaca
    11521 tcagcgatcc tgcccgttac cacgtagtga tggatgaact caacgatctg gaaatgggca
    11581 aaattctcgg agcctgtgaa ctcaccgtcg gtacgcgcct gcactccgcc attatctcaa
    11641 tgaactttgc tactccggcg attgccatca actatgaaca taaatccgcc gggattatgc
    11701 agcagctggg actgccagag atggcgattg atattcgtca tttactggac ggcagcctgc
    11761 aagcgatggt tgcggatacc ttaggccagc ttccggtgct gaacgctcga tttaacgaag
    11821 ccgtcagtcg cgaacgtcag acgggaatgc agatggtgca gtctgtgctt gagcgcatcg
    11881 gggaggtgaa atgaaggtcg gcttcttctt attgaaattc ccgctgtcgt cggaaacctt
    11941 cgtcctcaat caaattaccg cgtttattga tatgggcttt gaagtggaga ttgtcgcttt
    12001 gcaaaaaggc gatacagaga acacccacgc ggcatggacg aaatacaacc ttgtcgccag
    12061 aacccgctgg ttacaggacg aacctgcggg caaagtagcg aaactgcgcc accgcgccag
    12121 ccagacctta cgtggcattc atcgtaaaaa tacctggcat gcgctcaacc tcaaacgcta
    12181 tggtgccgag tcgcggaacc tgattttgtc tgccatttgt ggtcaggtcg caacaccgtt
    12241 tcgcgccgat gtgttcatcg ctcattttgg cccggcgggg gtaaccgcag caaaactacg
    12301 cgaactgggt atcattcgcg gcaaaattgc caccatcttc cacggtattg atatctccag
    12361 tcgggaagtg ctcaaccact acactcccga atatcaacaa ctgtttcgcc gtggcgacct
    12421 gatgttaccg ataagcgatc tgtgggccgg aaggctgcaa aaaatgggct gccccagaga
    12481 aaaaatcgcc gtatcgcgta tgggcgtgga catgacgcgc tttagcccgc gtccggtgaa
    12541 agcgcccgca acgccgctgg aaatcatatc tgtcgcacgc ttaaccgaga aaaaaggcct
    12601 gcatgtggcg attgaagcct gccgtcagtt gaaagagcag ggcgtggcat ttcgctatcg
    12661 catcctcggc attggcctgt gggaacgacg cctgcgcacc atcatcgaac aatatcaact
    12721 ggaagatgtg gtagagatgc cgggctttaa accgagccat gaagtgaagg agatgctcga
    12781 cgacgcggat gtcttcctgt tgccatcggt aacgggcgcg gatggcgata tggaaggcat
    12841 tccggtggcg ctaatggaag cgatggcggt cggtattccg gtggtttcta ctctgcatag
    12901 cggaattcct gaactggtgg aggccgataa atccggctgg ctgctgcctg agaacgatgc
    12961 tcgcgcactg gcacaacgtc tggcggcgtt tagccaactg gataccgacg aactggctcc
    13021 ggtcgtcaaa cgtgcgtgcg aaaaagtcga acacgatttt aaccagcagg tgattaatcg
    13081 agaactcgcc agcttgctgc aggctttata gaggagaacg tatgccattt aaaacactct
    13141 cccgacgcac cttcctgacg gcaagctcgg cgcttgcctt cctccatacc cctttcgctc
    13201 gcgcacttcc cgcccgacaa agcgttaaca ttaacgacta caacccacat gactggatcg
    13261 cctcatttaa acaagccttc agcgaagggc aaacggtcgt cgtgcctgcc ggattcgttt
    13321 gtgacaatat caacaccggc atcttcatcc ctcccggcaa aacgttacac atccttggaa
    13381 gcctgcgcgg caacggcaga gggcgatttg tcttacagga cggcagccag gtgacagggg
    13441 gagagggcgg cggtatgcat aacattaccc tggatgtgcg tggttctgac tgcaccatca
    13501 aagggctggc gatgagcggc tttggcccgg taatgcagat ttatattggc ggcaaaaaca
    13561 aacgggtcat gcgcaacctg accatcgata acctcactgt cagccacgct aattacgcca
    13621 tcttacgcca gggatttcat aaccagatta tcggtgccaa cattaccaac tgtaagttca
    13681 gcgacttaca gggcgacgcc attgaatgga acgtggcaat taacgacagt gatattttga
    13741 tatctgacca tgtcatcgag cgtatcaact gtaccaacgg caaaatcaac tggggcatcg
    13801 gcataggcct tgcgggaagc acttatgata acaactaccc ggaagaccag gcagtgaaaa
    13861 actttgtcgt ggcgaatatc acgggatcgg attgtcggca gttgatccat gttgaaaatg
    13921 gtaaacattt tgttattcgt aatatcaatg cccgcaatat cacgccggat ttcagtaaga
    13981 aagcgggcat tgataacgcg acagtcgcta tttacggttg tgacaatttc gtgattgata
    14041 atattgaaat gattaatagc gccgggatgt taatcggcta tggggtaatt aaaggcaaat
    14101 atctctcgat accgcaaaat ttccgagtga ataatattca actggataat acccatcttg
    14161 cttataaatt gcgcggcatc caaatctccg ccgggaatgc cgtctccttt gtgtcgctaa
    14221 ctaacattga gatgaagcgt gcctcgctgg agttacacaa caagccgcaa catcttttta
    14281 tgcgaaatat caatgtgatg caggaatcct cagttggacc agcattgagc atgaacttcg
    14341 acatgcgcaa agacgttcgc ggcgtcttta tggcgaaaaa agaaacactg ctgtctcttg
    14401 caaatgttca tgcggtgaat gaaagagggc aaagctccgt cgatatcgac aggattaatc
    14461 accatattgt taatgtggaa aagattaact ttagattgcc ggaacggagg gagtagattt
    14521 gcgaccattc ctggaaaaat ggagccatac ttaggaacaa tgctactgca atccacaacg
    14581 aagcggcgta acatcacaag taattcagta atcaattcag ggtaattgat gctggcgaaa
    14641 aaaatcgaac aagctataat tcagcaacca ttttacaggt ggatgaaata atgacgaatt
    14701 taaaagcagt tattcctgta gcgggtctcg ggatgcatat gttgcctgcc actaaggcga
    14761 tacccaaaga gatgctacca atcgtcgaca agccaatgat tcagtacatt gttgacgaga
    14821 ttgtggctgc agggatcaaa gaaatcctcc tggtaactca cgcgtccaag aacgcggtcg
    14881 aaaaccactt cgacacctct tatgagttag aatcactcct tgagcagcgc gtgaagcgtc
    14941 aactgctggc ggaagtacag tccatctgtc cgccgggcgt gatcattatg aacgtgcgtc
    15001 agggcgaacc tttaggttta ggccactcca ttttgtgtgc gcgacctgcc attggtgaca
    15061 acccatttgt cgtggtactg ccagacgttg tgatcgacga tgccagcgcc gacccgctac
    15121 gttacaacct tgctgccatg attgcacgtt tcaacgaaac gggccgcagc caggtgctgg
    15181 caaaacgtat gccgggtgac ctctctgaat actccgtcat ccagactaaa gagccgctgg
    15241 accgtgaggg taaagtcagc cgcattgttg aatttatcga aaaaccggat cagccgcaga
    15301 cgctggactc agacatcatg gccgtaggtc gctatgtgct ttctgccgat atttggccgg
    15361 aactggaacg tactcagcct ggtgcatggg gacgtattca gctgactgat gctattgccg
    15421 agctggcgaa aaaacaatcc gttgatgcaa tgctgatgac cggcgacagt tacgactgcg
    15481 gcaaaaaaat gggctatatg caggcgtttg tgaagtatgg cctacgcaac ctgaaagaag
    15541 gggcgaagtt ccgtaaaggt attgagaagc tgttaagcga ataatgaaaa tctgaccgga
    15601 tgtaacggtt gataagaaaa ttataacggc agtgaagatt cgtggcataa gttttgctca
    15661 ttgaattttc ctgccgttgc cgtttttata aagcctataa aaacaagaag ttacatagct
    15721 catattattc aaatttttcc aggattttcc ttgtttctac ggctgtttgg taagacaatt
    15781 aacgtttgaa tttttcggat ttggcgcgag tggctaacgc tcgccacatc gttgagatgc
    15841 atgcagtgca ctggtagctg ttaagccagg ggcggtagcg tgtattaata cctctattaa
    15901 tcaaatctgg agcagtctat ttcacagcat gctctatagc tatatggaat aaaaaagtga
    15961 agatacttgt tactggtggc gcagggttta ttggttctgc tgttgttcgt cacataataa
    16021 ataatacgca agatagtgtt gttaatgtcg ataaattaac atacgccggg aacctggaat
    16081 cactcgctga tgtttctgat tctgaacgtt atgcttttga gcatgcagat atctgcgatg
    16141 ctgtagccat gtcgcgtatt ttcgcacagc accagccaga cgcggtgatg cacctggcgg
    16201 cagagagcca cgttgaccgt tcaataacgg gccctgcggc atttattgaa accaatattg
    16261 tggggactta tgttctttta gaagcggcgc gcaattactg gtctgctctg aatgatgaaa
    16321 agaaaaaaag tttccgcttt catcatattt ctactgatga agtttatggt gacttacccc
    16381 atccggatga agcaaataat aacgaagcgt taccgctatt tacggaaacg acagcttacg
    16441 cgccaagtag cccgtattct gcgtctaaag cttccagcga tcatttggta cgtgcctgga
    16501 agcggactta tggtttacca accattgtga ctaattgctc gaacaattat ggtccttatc
    16561 acttcccgga aaaacttatt ccattggtta ttctgaatgc tctggaaggt aaggcattac
    16621 ctatttatgg taaaggggat caaattcgcg actggttgta tgtagaggat catgctcgtg
    16681 cgttatatac cgtcgtaacc gaaggtaaag cgggtgaaac ttataacatt ggtggacata
    16741 acgaaaagaa aaacatcgac gtagtgctca ctatttgtga tttgttggat gagattgtac
    16801 cgaaagagaa atcttatcgt gagcaaatta cttatgttgc cgatcgcccg gggcatgacc
    16861 gccgttacgc cattgatgcc gataaaatta gccgcgaatt gggctggaaa ccgcaagaaa
    16921 cgtttgagag cgggattcgt aaaacggtgg aatggtatct ggctaataca aattgggttg
    16981 agaatgtgaa aagcggtact tatcagtcgt ggattgaaca aaactatgag ggccgtcagt
    17041 aatgaatatc ctgcttttcg gcaaaacagg gcaggtgggt tgggaactgc agcgtgctct
    17101 ggctcctctg ggtaacttga ttgcgctcga tgttcactcc actgattatt gtggagattt
    17161 cagcaacccc gaaggtgtgg ctgaaaccgt caaaaaaatt cgccccgatg ttattgttaa
    17221 tgctgctgct cacactgcag tagacaaagc ggaatcagaa ccgaatttcg cgcaattact
    17281 taacgcgaca tgcgtcgaag cgattgcaaa agcagctaat gaagtcggag cctgggttat
    17341 acactactcc actgattatg ttttcccagg caacggtgac acgccatggc tggaaacgga
    17401 tgcaacagca ccgctaaatg tctacggtga aaccaagcta gctggggaaa aagcgttaca
    17461 agaacattgc gcaaagcatc ttattttccg taccagctgg gtatacgctg gtaaaggaaa
    17521 taactttgcc aaaacgatgt tgcgcctggc aaaagaacgc gaagagttgg ctgtaataaa
    17581 cgatcaattc ggcgcaccaa cgggggctga attactggct gattgcaccg ctcatgccat
    17641 tcgcgtggcc gcaaataaac cagaagttgc tggtttgtac catctggttg ctggtggtac
    17701 aacaacctgg cacgattatg ccgctctggt atttgaagaa gcgcgcagag cggggatcaa
    17761 ccttgctctt aacaaactta atgccgtgcc gacaacggcc tatcccacac cagcacgtcg
    17821 tccccataat tctcgcctca ataccgaaaa atttcagcag aactttgcgc ttgtcttgcc
    17881 tgactggcag gtgggcgtga aacgtatgct caacgaatta tttacgacca cggcaattta
    17941 acaaattatt gcatctcgct catgatgcta gagcgagatg aattaaaagg aatggtgaaa
    18001 tgaaaacgcg taaaggtatt attctggctg gtggttccgg cactcgtctt tatcctgtga
    18061 caatggctgt gagcaaacaa ctgctaccga tttatgataa accgatgatt tattatccgc
    18121 tttcaacgct tatgttggcg ggtattcgag atattctgat tatcagtacg ccgcaggata
    18181 caccacgttt tcaacaattg cttggtgatg gaagccagtg ggggcttaat ctgcaatata
    18241 aagtacaacc gagtccggat ggtctggcgc aagcatttat cattggtgaa gaatttatag
    18301 gtggtgatga ttgtgcgctg gtacttggtg ataatatctt ctatggtcac gacctgccga
    18361 aattaatgga taccgctgtt aacagagaga gtggcgcaac ggtttttgcc tatcacgtaa
    18421 atgatcctga gcgctacggt gtggtggaat ttgatgataa cggtacggca attagtctgg
    18481 aagaaaaacc gcaggaacca aaaagtaact atgcggttac tgggctttat ttctatgaca
    18541 atgacgttgt agaaatggcg aaaaacctta aaccatctgc acgtggcgaa ctggaaatta
    18601 ccgatattaa ccgtatttat atggaccaag gacgtttgtc tgtcgctatg atggggcgtg
    18661 gttatgcatg gctggacacg gggacacatc aaagtcttat tgaagcaagc aacttcattg
    18721 caacaattga agagcgtcag gggctgaaag tttcctgccc ggaagaaatt gctcaccgta
    18781 aaggctttat tgatgctgag caagtgaagg tattagccga gccgctgaag aaaaatgctt
    18841 atggtcagta tctgctgaaa atgattaaag gttattaata aaatgaacgt aattaaaact
    18901 gaaattcctg atgtgctaat ttttgaacca aaagtatttg gtgatgagcg gggctttttt
    18961 atggaaagct tcaatcagaa agttttcgaa gaggctgtag gacgaaaggt tgagtttgtt
    19021 caggataacc attctaaatc aactaagggt gtgttacgcg gactgcacta tcagctggaa
    19081 ccttatgctc aaggaaaact ggtacgctgt gttgtcggtg aagtttttga cgtagctgtt
    19141 gatattcgga aatcatcacc aacatttggc aagtgggttg gtgtaaattt gtcggcggag
    19201 aataagcgtc aattatggat accggaagga ttcgctcatg ggttttgcgt tcttagtgat
    19261 gaagcagaat ttgtctacaa aacaaataat ttctattcaa aaatgcaaga gcgtgggatt
    19321 ttatggagcg acaaaagtat aaatatagaa tggccggttc aaaat
//
LOCUS       DAZPCS010000066        19248 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZPCS010000066 DAZPCS010000000
VERSION     DAZPCS010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19248
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.066"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            146..1729
                     /locus_tag="ACHUQ7_002236"
     CDS             146..1729
                     /locus_tag="ACHUQ7_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFR1135635.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAKTASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(2181..4034)
                     /gene="asmA"
                     /locus_tag="ACHUQ7_002237"
     CDS             complement(2181..4034)
                     /gene="asmA"
                     /locus_tag="ACHUQ7_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFR1135636.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(4056..4637)
                     /gene="dcd"
                     /locus_tag="ACHUQ7_002238"
     CDS             complement(4056..4637)
                     /gene="dcd"
                     /locus_tag="ACHUQ7_002238"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFR1135637.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(4729..5370)
                     /gene="udk"
                     /locus_tag="ACHUQ7_002239"
     CDS             complement(4729..5370)
                     /gene="udk"
                     /locus_tag="ACHUQ7_002239"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFR1135638.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            5688..5930
                     /locus_tag="ACHUQ7_002240"
                     /pseudo
     CDS             5688..5930
                     /locus_tag="ACHUQ7_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
     gene            5929..6870
                     /gene="mdtA"
                     /locus_tag="ACHUQ7_002241"
                     /pseudo
     CDS             5929..6870
                     /gene="mdtA"
                     /locus_tag="ACHUQ7_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
     gene            6870..9992
                     /gene="mdtB"
                     /locus_tag="ACHUQ7_002242"
                     /pseudo
     CDS             6870..9992
                     /gene="mdtB"
                     /locus_tag="ACHUQ7_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
     gene            9993..13058
                     /gene="mdtC"
                     /locus_tag="ACHUQ7_002243"
     CDS             9993..13058
                     /gene="mdtC"
                     /locus_tag="ACHUQ7_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFR1135639.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLTPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNISIPKTFFPEQDTGVLMGGIQADQSI
                     SFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQQIID
                     RLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRKKLAT
                     LPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIYQPMN
                     QYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGLSAAS
                     TISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILIIAAI
                     ATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLIGIVK
                     KNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGS
                     ELRQPLEITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            13059..14474
                     /locus_tag="ACHUQ7_002244"
     CDS             13059..14474
                     /locus_tag="ACHUQ7_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1135640.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHISVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            14471..15874
                     /gene="baeS"
                     /locus_tag="ACHUQ7_002245"
     CDS             14471..15874
                     /gene="baeS"
                     /locus_tag="ACHUQ7_002245"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFR1135641.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            15871..16593
                     /gene="baeR"
                     /locus_tag="ACHUQ7_002246"
     CDS             15871..16593
                     /gene="baeR"
                     /locus_tag="ACHUQ7_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFR1135642.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            16784..17116
                     /locus_tag="ACHUQ7_002247"
     CDS             16784..17116
                     /locus_tag="ACHUQ7_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFR1135643.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            17263..18624
                     /gene="yegQ"
                     /locus_tag="ACHUQ7_002248"
     CDS             17263..18624
                     /gene="yegQ"
                     /locus_tag="ACHUQ7_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFR1135644.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(18966..>19248)
                     /locus_tag="ACHUQ7_002249"
     CDS             complement(18966..>19248)
                     /locus_tag="ACHUQ7_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1135645.1"
                     /translation="FLISGCAVHNDETSIGKFGLAYKSNIQRKLDNQYYTEAEASLAR
                     GRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFKCGE"
BASE COUNT         4327 a         5203 c         5168 g         4550 t
ORIGIN      
        1 taccgctttt aattcaggga taatgtgagg ttattaccct caaataatat gagataaata
       61 gtgctgcaac attgcatttt tgccccgatt tatccatgat cgaattgtga catttgtcat
      121 acaacgaata ggttttgtac ttactatgga atggattgcc gatccgtcta tctgggccgg
      181 gttaatcacg ctgattgtga tcgaactggt cctcggcatt gataacctgg tctttattgc
      241 catcctcgcc gaaaaactac cgccgaagca gcgcgaccgc gcacgggtta ccgggctgct
      301 gctggcgatg ttaatgcgcc tgttactgct agcgtcaatc tcctggctgg tcaccctgac
      361 tcaaccgctg ttcagcttcc gctcgtttac ctttagcgcc cgcgacttaa tcatgctgtt
      421 tggtggtttc ttcctgttgt tcaaagccac gatggagctg aacgaacggc tggaagggaa
      481 agacagcaat aatcccacac aacgcaaagg tgcgaagttc tggggcgtgg tgacgcaaat
      541 tgtggtactg gacgccattt tctcactcga ctcagtgatt accgccgtcg ggatggtcga
      601 ccatttactg gtcatgatgg ccgccgtggt tatcgctatc agcctgatgt tgatggccag
      661 caagccgtta acgcaatttg ttaacagtca cccgaccatc gttattctct gcttaagctt
      721 cctgttgatg attggcttta gcctggtggc agaaggtttc ggcttcgtca ttccgaaagg
      781 ctacctgtac gctgccattg gtttctcggt gatgatcgag gcactcaatc agttggctat
      841 ctttaaccgg cgacgttttc tttccgctaa tcagacgcta cgccagcgga ctaccgaagc
      901 ggtaatgcgc ctgcttagcg ggcaaaaaga agatgcggaa ctggacgcca aaaccgcgtc
      961 catgctggtg gatcatggaa accagcagat ctttaatccg caggaacggc ggatgattga
     1021 gcgggtactt aatcttaacc agcgtaccgt aagcagcatt atgacgtcgc gccacgatat
     1081 tgagcatatc gatctcaacg cgccggaaga agaaatccgc cagttgctgg agcgaaatca
     1141 gcatacgcgg ctggtggtta ccgacggtga tgacgcagaa gatttgctcg gtgttgttca
     1201 cgttatcgac ctgctacaac agtcactgcg cggcgaaccg ctcaacctgc gggtgttgat
     1261 tcgccagccg ctggtgttcc cggaaacctt gccgttgtta ccagccctgg agcagttccg
     1321 taatgcccgc acgcactttg cttttgtggt ggatgagttt ggctcggtgg aagggattgt
     1381 gacattaagt gacgtcactg aaaccattgc cggtaactta ccgaacgaag tggaagagat
     1441 cgacgcccgc catgatattc agaagaatgc ggacggttcc tggacggcga atggtcatat
     1501 gccgctggag gatctggtgc aatatgtgcc gctgccgctg gatgaaaaac gtgaatatca
     1561 caccattgcc gggctgttga tggagtattt gcagcgtatt ccaaaacctg gcgaagaagt
     1621 tcaggtgggg gattatttgc ttaaaacgtt gcaggtggaa agccatcgcg tgcagaaggt
     1681 gcagattata ccgctgcgta aggatggcga gatggagtac gaggtgtgat ggtatcggcc
     1741 attttaccca atgctgcgta acgccttata acttcagcaa tgaagcgttg tctgtggtaa
     1801 acggacatca cagcgggggt gtgggtccgg ctgtaaatcg ccgaatcgtt tcctgtaggc
     1861 tggggcagcc cacatggatg tgggctgagg gcgcgtttta cagggatgtt acctcgcgcc
     1921 cgtacccgat agccgaaagg aataagatga gggaaccgcg ccagcggcga tttaccgccg
     1981 ggagcccggg ttgccagggt ggtggcgatt gagccaccct ggcacgttca ccggcttgag
     2041 cgttccatag tagcgaggaa catcaagtga acggaacagc cttcgatctt acatattcag
     2101 aatataatag tgccgccgcg aacgcctgat gcgacgcttt acgcgtctta tcaggcctac
     2161 aggacaggtc acctggtaac ctacatcttc tccagcaact tcttcacatc tttgccattg
     2221 cgggaatctt tattccgctc ggcccagtca ttcaggcgac gtttcgcttc gtcctgtaga
     2281 tgtttgcgca gtaactgatc cacttgcagg ctgtaattga gttgctgcca gttgccataa
     2341 acccgcagcg gtactggcgt ttctttgagg aaatcaatca gtttgctttc cccgttccag
     2401 ccacccacga cccgaatatc aaactgggtg tcgcaggttt gatctgccag attcaacatg
     2461 ccttcccctg tcagcgccag cacaggcgat tgaccttgca tgtcgtttaa cgtcacgacg
     2521 ccatccttca acgtcaaatc ggtggtaaag cggtcaagcc gcgtcacgtt atcgaagttt
     2581 tcagcggcct tcacatcacc gccattacgc tctaccgctt gctgaatcat ctgctggaag
     2641 ttcatccctt ccatacgtgt gtcggtcatc tcaacatgcg cctgtccttg ccagttgtgg
     2701 cggaatgcgt cggcatctat gtcagcaccg gagaaatcac cagccagtga catttttccg
     2761 gtcaacgaaa tcggatagtt aaacgccttc agaatggtac caatctcaac gttctccagc
     2821 cgtggctgga agtttatccg cggatttatt gatgtcgcgt ccagcgtgcc cggcagtgaa
     2881 acctttccac cgttaagctt gccctgcagt tgagtaattt ccagcaaacc cgacttgttg
     2941 gtcatttgcg tggcaacatc tgtaaaattc attccgcgcc agcgcacgtt actggcctgc
     3001 aacaaaatat cagctgtaaa gccttgcagt ccctgatagg ccggttcatc aatacgcgaa
     3061 gaaatgaccg ggcgcggcaa cgttgattgg ctctgcccct gctgcgcggc accgttttca
     3121 ccattcgcgg tttcattaag cgggatgagg ttgtcaagat tcagttgcgg gaattgcagt
     3181 ctcagctgcc attccggttt ctctgtcagc gtgacctgtg cttgtccgct cagcgtactg
     3241 tcattggcgg tcaaactaat ttggttaaat gaaaggcgtt tatgtgactc ctgccactgg
     3301 gactggaaac tcccctgccc ctgaatacct tgttttggta aatcggcacc ctgcaactgc
     3361 cagttaattt gttcaatagc cgctgttaaa tcatgcggat aatcagaagc atctaccgta
     3421 ccgttaaggg atatcgtgag atcgcgctga tcgcgattaa cccgcccgga gaactcaaat
     3481 gagccacgat gttggggatc ttgttccatt tgcaggcgga tattgcggat tgtcacctgc
     3541 tcgtcatctt catgctggaa caccagaacg ctgtccgcca ccttaagact ggatatatca
     3601 aacgaccatc cgcgatcgtc tgacagatcc ggcaaggtat tgtcgcgcgg tgcaaccgga
     3661 gcgtcttcac tgcgcaccgc ttccgtctgc ggcgtcagtt ggatcactgc cccttttagc
     3721 atcacctgct taacgctcag ttgatgactc agtagtggta aaagcgccac gtccagacgc
     3781 atgttgtcgg cgcgaaccag tggctggctt gcgccctggg cggtgagaga cattcgcccg
     3841 gagaggatac taagctgcgg ccagacgtgc caacgcagtg gcccgtcgag ctgcaattga
     3901 taaccgctac gtgcagcaac ttgcttgacc atatagtcgc ggaaatcatt cggattcacc
     3961 agcaacacta acgcagataa cccggccacc agcacgacca agagtatcat cagcgtcgtc
     4021 agaaatcgtc tcatggtatc ctcaatgggc ctgaattagt ctttatcgat tcggctggct
     4081 accgcgccct gctggttgcg atatttcgca tcttcacggc ggttgtaagg tcgcgccgcc
     4141 gggccggaaa gcggctcaaa gctcagcgca ccaattaaca tgcccggacg cagcgccagc
     4201 ggcagcttac cggagttgta gaactccagc acaatgcaac cagaccagcc cggatcgata
     4261 cggtgcgcgg tgacgtgcac catcagcccc agacgcgcca aagaggaacg cccgtccagc
     4321 cagcccacca gatcggctgg cagcgtcacc gactccagtg tcaccgccag cgccagctct
     4381 cctgggtgaa gatagaacgc ctcgccctcg tcgagaacga tctcatcgct catcacgcgg
     4441 tcaagcgcgg cgctcacttc atctttggga ccgctcagat cgataaacgc tgccgtgtga
     4501 ccacggaagg tacgaaattt attgcccagg cgtacatcca ccgtcgcgcc gttaatacgc
     4561 tccactggcg gacgtgggtt gatcgacaaa cggccttcat caagccaggc ttcaatatct
     4621 cggtcacaca gacgcatggc attttctcct ttcgcacatc actcccttaa cgccaattac
     4681 gtcaagggca taccaactta tcactgaacg gtacacaatt tatcaagctt attcaaagaa
     4741 ctgacttatt ttcgctttca atatatcgat cgcgatgcgg tttttcccgc cgcgcggcac
     4801 gataatgtcc gcatattgtt tagaaggctc aatgaattgc aggaacatcg ggcgcacggt
     4861 tttttgatat tgcgccatca cggaatccat tgaacgccca cgctcgttaa cgtcacgctt
     4921 gatgcggcgc atcaggcaga tatccagcgg ggtatcaacg aaaatggaga agttaagttc
     4981 gtcacgcaaa cgcgcatccg tcagcaacaa aatgccttcg agaatgatga ccttcttcgg
     5041 ctcaaccgtc accgtttctt tcatacgcgt atgttcaaca tagctgtaaa ccggcaggtc
     5101 aattgccgag ccgcgtttca acgcttgtaa atgctcaagc aacaggctgt gatccatcgc
     5161 gctgggatgg tcgtagttgg tcttaacgcg ttcttccatc gacagatggc tttgatcttt
     5221 gtaatagcag tcttcgggaa ttacgccgat gtgttcatca ccgacttgct cacgtaattc
     5281 acgataaagg gtactggcaa taagactctt gccggaagcc gatgcgccag cgataccgat
     5341 aatgacgcac tgatgagact gatcagtcat atatttagcg acctgattaa cctggatgtt
     5401 aggaaggggg cgacgaagcg ccaaacgcgg caattatagg gatttcatcc gtctgatacc
     5461 agtcgaatag cgttgccgcg cgctcagagt taattgttga caaagaattc ccggtggcaa
     5521 attacgttga tcagttttat gtaaggtaaa aaatgttata cgcagttgcg caaattatcc
     5581 gcctttacgt cactttatga gcaattcgca tataaaatgt aaaacttttg tactagcata
     5641 aacacagaaa cgaatactgg cgacctggcc ttgcggataa agcggtaata agcaaacaat
     5701 cacagcatgt attaattgcc ctgccccacc cgctgcttca cctggtcagt ttaggtttag
     5761 tctcgtttat ctttaccctt gtctcgcttg agctttcgca gtttggcacc caactcgccc
     5821 cactgtggtt cccgacgtcc atcatgatgg tggcgtttta tcgccatgcc gggcgcatgt
     5881 ggccgggaat tgcgctgagc tgttcgctgg gaaatatcgc cgcatccatt ccaggaaggc
     5941 cagcaggtca aagcaggcga tttactggca gaaattgacc ccagccagtt caaagttgca
     6001 ttagcacaaa cccagggcca actggcaaaa gataaagcca cgcttgctaa cgcccgccgt
     6061 gatctggcgc gttatcagca actggcaaaa accaatctcg tctcccgtca ggagctggat
     6121 gcccaacagg cgctggtcag tgaaaccgaa ggcaccatta aggctgatga agcaagcgtc
     6181 gccagcgcgc agttgcaact cgactggagc cgcatcaccg caccagtcga tggtcgcgtt
     6241 ggtctcaagc aagttgatgt tggtaaccaa atctccagtg gtgataccac cggaattgtg
     6301 gtgatcaccc agacgcatcc tatcgattta gtctttaccc tgccggaaag cgatatcgct
     6361 actgtagtgc aggcgcaaaa agccggaaaa ccgctgatgg tagaagcctg ggatcgcacc
     6421 aattcgaaga aattaagtga aggcacgctg ttaagcctcg ataaccaaat cgatgccaca
     6481 accggtacga ttaaagtgaa agcacgcttt aataatcagg atgatgcgct gtttcccaat
     6541 cagtttgtta acgcgcgcat gttagtcgac accgaacaaa acgccgtagt gatcccaaca
     6601 gccgccctgc aaatgggcaa tgaaggccat tttgtctggg tgctgaatag cgaaaacaag
     6661 gtcagcaaac atctggtgac gccgggcatt caggacagtc agaaagtggt gatccgtgca
     6721 ggtatttctg cgggcgatcg cgtggtgaca gacggcattg atcgcctgac cgaaggggcg
     6781 aaagtggaag tggtggaagc ccagagcgcc accactccgg aagagaaagc caccagccgc
     6841 gaatacgcga aaaaaggagc acgctcctga tgcaggtgtt acccccgagc agcacaggcg
     6901 gcccgtcgcg cctgtttatt atgcgtcctg tcgccaccac gctgctgatg gtggcgatct
     6961 tactcgccgg gattatcggt tatcgcgccc tgcccgtttc ggcgctgccg gaagtggact
     7021 atccgaccat tcaggtggtc acgctctacc caggtgccag cccggatgtc atgacctctg
     7081 ccgttaccgc gccgctcgaa cgccagttcg ggcagatgtc tggcctgaaa cagatgtcgt
     7141 cgcaaagttc cggcggtgcg tcagttatca ctttgcagtt ccagctaaca ttaccgctca
     7201 atgtcgccga gcaggaagtg caggccgcga ttaacgctgc gaccaacttg ttgccgagcg
     7261 atctgcctaa cccgccggtt tacagcaaag tgaacccggc agatccgccg atcatgacgc
     7321 tcgccgtcac ctcaaccgcc atgccgatga cgcaagtgga agatatggtg gaaacccgcg
     7381 tcgcgcagaa aatctcgcag atttccggcg tcggcctggt gacgctttcc ggcggtcagc
     7441 gtccggctgt tcgcgtcaaa cttaacgctc aggcgattgc cgccctcggc ctgaccagcg
     7501 aaaccgtgcg caccgccatt accggcgcta acgttaactc ggcaaaaggt agcctcgacg
     7561 gcccttcccg tgcggtcacg ctttccgcga acgaccagat gcaatccgcc gaagagtatc
     7621 gccagctaat catcgcctac cagaacggcg cgccaattcg tctgggcgat gtcgcaactg
     7681 tagagcaagg tgcagaaaac agctggctcg gcgcgtgggc gaacaaagaa caggccattg
     7741 tgatgaatgt tcagcgccag cccggtgcta acattatctc caccgccgac agcattcggc
     7801 agatgctgcc acagctcact gagagtctgc cgaaatcggt gaaggtgaca gtgctttccg
     7861 atcgcaccac caatatccgc gcatccgtcg atgatactca gtttgaattg atgatggcta
     7921 tcgcgctggt agtcatgatt atctacctgt ttttgcgcaa tattccggcg accatcattc
     7981 ccggtgttgc tgtaccgctg tcgttaatcg gcactttcgc ggttatggtg tttctcgatt
     8041 tttcaatcaa taacctgaca ctgatggctt taactatcgc caccggattc gtggtcgatg
     8101 acgccatcgt ggtgatcgaa aacatttccc gctatatcga aaaaggcgaa aaaccgttgg
     8161 cggcggcgct caagggcgca ggtgaaatcg gctttaccat tatctcgctg accttctcac
     8221 tgattgcggt gttgatccca ctgctgttta tgggcgatat cgtcgggcga ctgttccgcg
     8281 aatttgctat taccctggcg gtagcgattt tgatctcagc ggtggtgtcg ctgaccctga
     8341 caccgatgat gtgcgcgcgg atgctcagcc aggagtcgtt gcgtaaacag aacagcttct
     8401 cccgtgcctc ggaaaaaatg ttcgacagga taatcgccgc ctatggtcgt ggactggcga
     8461 aagtgctgaa tcatccgtgg ctgaccttaa gcgtggcact cagcacgctg ctgcttagcg
     8521 tgctgctgtg ggtgttcatt ccgaaaggtt tcttcccggt acaggacaac ggcattattc
     8581 agggcacttt gcaggcaccg caatccagct cctttgccaa tatggcccag cgacaacgcc
     8641 aggtcgcgga cgtgattttg caggatccgg cagtgcaaag cctgacctca tttgttggcg
     8701 ttgatggcac taacccgtcg ctgaatagtg cacgtttaca aatcaacctc aaaccgttgg
     8761 atgaacgtga tgatcgggtg caaaaagtca tcgcccgtct gcaaacggcg gtagataaag
     8821 tgccgggcgt cgatctcttc ctgcaaccaa cgcaggacct gaccatcgat actcaggtca
     8881 gccgcaccca gtaccagttt accttgcagg ccacgtcact ggatgcgctc agtacctggg
     8941 tgccacagtt gatggaaaaa ctccagcaac tgccacagct ttctgatgtc tccagcgact
     9001 ggcaggacaa agggctggtg gcgtatgtca atgttgatcg cgacagcgcc agccgtctgg
     9061 ggatcagcat ggcggacgtc gataacgccc tgtacaacgc gtttggtcag cgactgattt
     9121 ccactattta tactcaggct aaccagtatc gcgtggtgct ggagcacaat accgaaaata
     9181 ccccaggcct cgcggcgctg gataccattc gcctgaccag cagcgacggt ggcgtggtgc
     9241 cgttaagctc aattgccaaa attgagcagc gttttgcgcc gctttccatc aaccatctgg
     9301 atcagttccc ggtaacaacc atctccttta acgtgccgga taactattcg ctgggcgatg
     9361 cggtgcaggc gattatggac accgaaaaga cgctgaatct gccggtggat atcaccacgc
     9421 aattctaggg cagcaccctc gccttccagt cggcgctggg cagcactgtc tggctgattg
     9481 tcgcggcggt ggtggcgatg tatatcgtgc tcggcattct gtacgagagc tttattcacc
     9541 cgatcaccat tctctcgacg ctacccaccg caggggtcgg cgcgctgctg gcattaatga
     9601 ttgccggtag cgaactggat gtgattgcga ttatcggcat tattttgctg atcggtattg
     9661 tgaagaagaa cgccatcatg atgatcgact tcgcgctggc tgctgagcgc gagcaaggca
     9721 tgtcgccgcg cgatgcaatc taccaggctt gtctgttgcg ttttcgtccg atcctgatga
     9781 ccaccctggc ggctctgctt ggcgcgctgc cgctgatgtt gagtaccggg gtcggcgcgg
     9841 aactgcgtcg tccgttaggt atcggcatgg tcggcggtct gattgtcagc caggtgctga
     9901 cgctgttcac cacgccggtg atttatctgc tgttcgaccg cctggcattg tggaccaaaa
     9961 gtcgctttgc tcgccatgaa gaggaggcgt aagtgaagtt ttttgccctc ttcatttacc
    10021 gcccggtggc gacgatttta ctgtcggtcg ccattaccct gtgcggcata ctgggtttcc
    10081 gtatgctgcc ggtcgccccg ctgccgcagg tcgattttcc ggtgattatg gtcagcgcct
    10141 cgctgcccgg tgcgtcgccg gagacaatgg cgtcttccgt tgccacgccg ctggagcgct
    10201 cacttgggcg cattgccggg gttagtgaaa tgacctccag cagttcgctc ggcagcacgc
    10261 gcattatttt gcagtttgat tttgaccggg atatcaacgg cgcagcgcgt gatgtgcagg
    10321 cggcgatcaa cgctgcacaa agtttgctgc ccagcgggat gcccagccgc ccgacctatc
    10381 gcaaagcgaa cccgtcggat gcgccaatta tgatcctcac gctgacatcc gatacttatt
    10441 cgcagggtga actgtacgat ttcgcctcga cgcagctggc tccgacgatt tcgcaaatcg
    10501 acggtgtcgg tgatgtcgat gtcggtggca gctcgctgcc cgccgtgcgc gtcgggctga
    10561 ccccgcaggc gctgtttaat cagggcgtgt cgctggacga cgtacgcact gccatcagca
    10621 atgccaacgt gcgtaaaccg cagggggcgc tggaagatgg cactcaccgc tggcagatcc
    10681 agaccaacga tgaactgaaa accgccgctg aatatcagcc gctgattatt cactacaaca
    10741 acggcggcgc ggttcgtctg ggcgatgtgg cgacggtgac tgactcagtg caggatgtgc
    10801 gcaacgccgg gatgaccaac gccaaaccgg ctattttact gatgatccgc aaactgccgg
    10861 aagccaatat tatccagacg gtagacagca tccgggcaaa attaccagag ttgcaggaga
    10921 ccattccggc ggcgattgat ctgcaaattg cccaggatcg ctcccccacc attcgcgcct
    10981 cgctggaaga agtcgagcaa acgctgatta tctcggtggc gctggtgatt ctggtggtct
    11041 ttttattcct gcgctcgggt cgcgccacta ttattcccgc cgttgcggtg ccggtttcgc
    11101 tgattggtac gtttgcggcg atgtacctgt gcggattcag tctcaataat ctttcgctga
    11161 tggcgctcac cattgctact ggtttcgtgg tggatgacgc cattgtggtg ctggaaaaca
    11221 ttgcccgtca tctggaagcg gggatgaaac cactacaggc cgcgctgcaa ggtactcgcg
    11281 aagtcggctt tacggtgctg tcgatgagtc tgtcactggt ggcggtgttc ctgccgctgc
    11341 tgttgatggg cggattgccg ggccgactgt tacgcgagtt tgccgtgacg ctttctgtcg
    11401 ccattggtat ttcgttgctg gtttccctga cgctaacgcc aatgatgtgc ggctggatgc
    11461 taaaagccag caagccgcgt gagcaaaagc gactgcgtgg ttttggccgc atgttggtag
    11521 ccctgcaaca aggctacggc aagtcgctga aatgggtgct caatcatacc cgtctggtgg
    11581 gcgtggtgct gcttggcacc attgcgctga atatctcgat cccgaaaacc ttcttcccgg
    11641 agcaggacac cggcgtactg atgggtggga tacaggcgga tcagagtatt tcgtttcagg
    11701 cgatgcgcgg caaattgcag gatttcatga aaattatccg tgacgatccg gcagtggata
    11761 atgtcaccgg ctttaccggc ggttcacgag ttaacagtgg gatgatgttt atcaccctca
    11821 agccacgcga cgaacgcagc gaaacggcgc agcaaattat cgaccgtctg cgcgtgaagc
    11881 tggcgaaaga accgggggcg aatctgttcc tgatggcggt acaagatatt cgcgttggtg
    11941 ggcgtcagtc gaacgccagc taccagtaca cgttgttatc cgacgacctg gcggcactgc
    12001 gcgaatggga gccgaaaatc cgcaaaaaac tggcgacgtt gccggaactg gcggacgtga
    12061 actccgatca gcaggataac ggcgcggaga tgaatctggt ttacgaccgc gacaccatgg
    12121 cacggctggg aatcgacgta caggccgcca acagtctgtt aaataacgcc ttcggtcagc
    12181 ggcaaatctc gaccatttac cagccgatga accagtataa agtggtgatg gaagtggatc
    12241 cgcgctatac ccaggacatc agtgcgctgg aaaaaatgtt cgttatcaat aacgaaggca
    12301 aagcgatccc gctgtcgtat ttcgctaaat ggcaaccggc gaatgcccca ctatcggtga
    12361 atcatcaggg attatcggcg gcctcgacca tctcgttcaa cctgccgacc ggaaaatcgc
    12421 tctcggacgc cagtgcggcg atcgatcgcg caatgaccca gcttggtgtg ccttcgacgg
    12481 tgcgcggcag ttttgccggc acggcgcagg ttttccagga gacgatgaac tcgcaggtga
    12541 tcctgattat cgccgccatc gccacggtgt atatcgtgct gggtatcctt tacgagagtt
    12601 acgtacatcc gctgacgatt ctctccaccc tgccctcggc gggcgttgga gcgctgttgg
    12661 cgctggagct gttcaatgcc ccgttcagcc taatcgccct gatagggatc atgctattaa
    12721 tcggcatcgt gaagaaaaac gccattatga tggtcgattt tgcgcttgaa gcccaacggc
    12781 acggtaacct gacgccgcag gaagctattt tccaggcctg tctgctgcgt tttcgcccga
    12841 ttatgatgac caccctggcg gcgctgtttg gcgcgctgcc gctggtattg tcgggcggtg
    12901 acggctcgga gctgcggcaa cccctggaga tcaccattgt cggcggtctg gtgatgagcc
    12961 agctcctgac gctgtatacc acgccggtgg tgtatctctt tttcgaccgt ctgcggctgc
    13021 gtttttcgcg taaacctaaa caaacggtaa ccgagtaaat gacagatctt cccgacagca
    13081 cccgctggca attatggatt gtggctttcg gcttctttat gcagtcgctg gacaccacca
    13141 tcgtaaacac cgcccttccc tcaatggcgc aaagcctcgg ggaaagtccg ttgcatatgc
    13201 acatggtcat tgtctcttat gtgctgaccg tggcggtgat gctgcccgcc agcggctggc
    13261 tggcggacaa agtcggcgtg cgcaatattt tctttaccgc catcgtgctg tttactctcg
    13321 gttcactgtt ttgcgcgctt tccggcacgc tgaacgaact gttgctggca cgcgcgttac
    13381 agggcgttgg cggtgcgatg atggtgccgg tcggcaggtt gacggtgatg aaaatcgtac
    13441 cgcgcgagca atatatggcg gcgatgacct ttgtcacgtt acccggtcag gtcggtccgc
    13501 tgctcggtcc ggcgctcggc ggtctgctgg tagaatacgc gtcgtggcac tggatctttt
    13561 tgatcaacat tccggtgggg attatcggtg cgatcgccac attgatgtta atgccgaact
    13621 acaccatgca gacgcggcgc tttgatcttt ccggcttttt attgctggcg gttggcatgg
    13681 cggtattaac cctggcgctg gacggcagta aaggtacagg tttatcgccg ctggcgattg
    13741 ctggcctggt cgcagttggc gtggtggcac tggtgcttta tctgctgcac gccagaaata
    13801 acaaccgcgc cctgttcagt ctgaaactgt tccgcactcg taccttttcg ctgggcctgg
    13861 cggggagctt tgccggacgt attggcagtg gcatgttgcc ctttatgaca ccggttttcc
    13921 tgcaaattgg cctcggtttc tcgccgtttc atgccggact gatgatgatc ccgatggtgc
    13981 ttggcagcat gggaatgaag cgaattgtgg tacaggtggt gaatcgcttt ggttatcgtc
    14041 gggtactggt agcgaccacg ctgggtctgt cgctggtcac cctgttgttt atgactaccg
    14101 ctctgttggg ctggtactac gttttgccgt tcgtcctgtt tttacaaggg atggtcaact
    14161 cgacccgttt ctcctccatg aacaccctga cgctgaaaga tctcccggac aatctggcga
    14221 gcagcggaaa cagcctgctg tcgatgatta tgcaattgtc gatgagtatc ggcgtcacca
    14281 tcgccgggct gttgctgggg ctttttggtt cgcagcatat cagcgtcgac agcggcacca
    14341 cacaaaccgt ctttatgtac acctggctta gcatggcgtt tatcatcgcc cttccggcgt
    14401 tcatctttgc cagagtgccg aacgatacgc atcaaaatgt agctatttcg cggcgaaaaa
    14461 ggagcgcgca atgaagttct ggcgacccgg tattaccggc aaactgtttc tggcgatttt
    14521 cgccacctgc attgtcttgc tgatcagtat gcactgggcg gtgcgtatca gttttgagcg
    14581 tggctttatt gattacatca aacacggcaa tgaacagcga ttacagctgt taagtgatgc
    14641 gcttggcgag cagtatgcgc agcatggtaa ctggcgcttc ctgcgcaaca acgatcgctt
    14701 tgtctttcag atcctgcgtt catttgaaca cgataattcg gaagataaac ccggcccggg
    14761 tatgccgccg cacggctggc gtacccagtt ctgggtggtc gatcaaaaca acaaagtact
    14821 ggttggtccg cgagcgccga ttccacctga cggtacacgg cgacccattc tggtcaacgg
    14881 tgcggaagtg ggcgcggtga ttgcctcccc tgttgagcgg ctgacccgca atactgatat
    14941 caatttcgat aaacaacagc ggcaaaccag ctggctgatc gtcgccctgg caacgttact
    15001 cgcggcgctc gccacctttc tgctggcgcg cggtttgctg gcaccggtaa aacgacttgt
    15061 cgatggcacg cacaaactgg cggcgggcga tttcactacc cgcgtaacgc ccaccagtga
    15121 agatgaactg ggcaaactgg cgcaagactt caaccagctc gccagcacgc tggagaaaaa
    15181 ccagcaaatg cggcgcgatt ttatggccga tatttctcac gaactgcgta cgccattagc
    15241 ggtgctgcgc ggtgaactgg aagccattca ggatggcgtg cgcaaattca cgccggagac
    15301 ggtggcgtct ttacaggcgg aggtcggtac actgaccaaa ctggttgacg atcttcatca
    15361 gttgtcgatg tctgatgaag gcgctctcgc ctatcaaaaa gcaccggtag atttgatccc
    15421 actgctggaa gtggcgggcg gcgcatttcg cgaacgattc gccagccgtg gcctgaaact
    15481 gcaattttcc ctgccagaca gtatcaccgt atttggcgat cgcgaccgtt taatgcagtt
    15541 attcaataac ttactggaaa acagcctgcg ctacactgac agcggcggca gcctgaaaat
    15601 ctctgccgag cagcacgaca aaacggtgcg cctgaccttt gccgacagtg cgccaggtgt
    15661 cagtgacgat cagctacaaa aattgtttga acgtttttat cgcaccgaag gttcccgcaa
    15721 ccgcgccagc ggcggttccg ggctggggct ggcgatttgc ctgaacattg ttgaagccca
    15781 taatggccgc attattgccg cccattcgcc ttttggcggg gtaagcatta cagtagagtt
    15841 accgctggaa cgggatttac agagagaagt atgaccgagt taccaatcga cgaaaacaca
    15901 ccgcgtattt tgatcgtgga agatgaaccg aagctggggc agttgctcat tgattatctg
    15961 cgtgctgcga gctatgcgcc gacgcttatc agccacggcg atcaggtact ggcgtatgtg
    16021 cgccagacac caccggatct gatcctgttg gatctgatgc tccctggcac cgatggcctg
    16081 acattatgcc gggaaattcg tcgtttttcc gatgttccga tcgtgatggt gacggcaaaa
    16141 atcgaagaga tcgaccgcct gctggggctg gagattggcg ccgatgacta catctgcaag
    16201 ccgtacagcc cacgggaagt ggtagcgcgt gtcaaaacca ttttgcgccg ttgtaaaccg
    16261 caacgcgaac ttcagcaaca ggatgccgaa agcccgttga ttatcgacga aggtcgtttt
    16321 caggcttcat ggcgcggtaa aatgcttgac ctgacgcctg cggaatttcg tctgcttaaa
    16381 acgctctctc acgaaccagg aaaagtgttc tcccgcgagc aattgctcaa tcatctttat
    16441 gacgactacc gcgtagtaac cgaccgcacc attgacagcc acattaaaaa tctgcgccgc
    16501 aagctggaat ctctcgacgc cgaacagtca tttatccgcg ccgtttacgg cgttggttac
    16561 cgctgggaag ccgacgcctg ccgcatcgtt tagttttagc gacattactt tgttagccgg
    16621 agatggcgtt cagacccgat ccggcaacat tattccacgc atggtcagca aactgacatt
    16681 atctctccgc cctggcaggc atcagaaaat cgctcatact ttaatcagta aacagcacct
    16741 ttagatgctg ttttcgataa gcaatttcaa tcaaggagtc attatggctg gttggtttga
    16801 actcagtaag agcagtgata atcagttccg gtttgtgcta aaagcgggca atggtgagac
    16861 tatcctcacc agcgagctgt atacctcaaa agcctctgcg gaaaagggca tcgcgtcggt
    16921 gcgtagcaac agcccgcaag aagagcgcta tgagaaaaaa acggcaagca acggcaaatt
    16981 ctatttcaat ctgaaagccg ctaatcatca aattatcggc tccagccaga tgtacgccac
    17041 cgcgcaatct cgtgaaaccg gaattgcctc cgttaaagcc aatggcacaa gccagacggt
    17101 gaaagacaat acgtaattac gatgccgggc gtgggttgtt ggcgtccggc gctttaccag
    17161 atccccccta cagcgctaca atgcccgccc ttaaagtggg ggcctcccct aaccgcttca
    17221 tcaggtgaag cggatctgac ctgtcatcag aacgagagaa ttatgtttaa accggaactc
    17281 ctttccccgg cgggaacgct gaaaaatatg cgttacgctt tcgcttatgg cgcagatgct
    17341 gtttatgcgg gccagccgcg ttactcccta cgtgtgcgca acaacgaatt caaccacgaa
    17401 aatcttcagc tcggcatcaa tgaagcccac gcgctgggga aaaagtttta tgtcgtggtc
    17461 aacattgcac cgcacaacgc caagctgaaa acctttatcc gtgacctgaa accggtggtg
    17521 gaaatggggc cggatgcgct gattatgtcc gatccagggc tgattatgct ggtgcgtgag
    17581 cacttccctg aaatgccgat ccacctctcg gtgcaggcta acgccgtgaa ctgggcgacg
    17641 gtgaaattct ggcagcaaat gggactgacc cgcgtgatcc tctctcgcga gctgtcgctg
    17701 gaagagattg aagagatcca taatcaggtg ccggatatgg agatcgagat cttcgttcac
    17761 ggcgcactgt gcatggccta ctccggtcgc tgcctgctct ctggctatat caacaagcgc
    17821 gacccgaacc agggcacctg caccaacgcc tgccgctggg agtacaacgt ccaggaaggg
    17881 aaagaagatg acgttggcaa catcgtacac aagtacgagc cgattccggt gcaaaatgtt
    17941 gagccgacgc tgggtatcgg cgcgccaacc gacaaagtgt ttatgatcga agaagcccag
    18001 cgtccgggcg agtatatgac cgcgtttgaa gatgagcacg gcacttacat catgaactcg
    18061 aaagatctgc gcgccatcgc ccatgtagaa cgcctgacca aaatgggcgt gcattcgctg
    18121 aaaatcgaag gtcgtaccaa atctttctac tattgcgcac gcaccgcaca ggtttaccgc
    18181 aaagctatcg atgacgccgc tgcgggaaaa ccgttcgata ccagcctgct ggaaactctg
    18241 gaaggtctgg cgcatcgtgg ctataccgaa ggtttcctgc gtcgtcatac tcacgacgat
    18301 tatcagaact acgaatacgg ttattcagtt tctgaccgcc agcagtttgt tggtgagttt
    18361 accggtgagc gcaaggggga cctcgcagcg gtagcggtga aaaataaatt ctccgttggc
    18421 gacagtcttg agctgatgac gccgcaaggc aacattaact ttacccttga gcacatggaa
    18481 aacgccaaag gcgaagctat gccggtcgca ccaggcgatg gttatactgt gtggctcccg
    18541 gtcccgcagg atcttgagct aaattacgcg ctgctaatgc gtaatttctc cggggaaacc
    18601 acgcgtaacc cccacggtaa gtgattaatt tcgattattt ttcccggatg gaaaattctt
    18661 agaaaccgat cacatacagc tgcatttatt aaggttatca tccgtttcgc tgaaaaacat
    18721 aatccataaa atgctagctg taccaggaac cacctcctta gcctgtgtaa tctcccttac
    18781 acgggcttat tttttgtaca taagctgtaa agaaggattg atccctgata accgtcttcc
    18841 attgaccaca tcgatagaat cccccttcat agcacaatgc ctttcacgta acggtatcgt
    18901 gctcgcccag gttccggcta cgcacagcca gaacgcgcat gtttgacgct tacaaaaaaa
    18961 tattctcact ctccacattt gaatgtcaga cgagcgacgc catgtaatcc tgcaccttct
    19021 gtcttcaggt caactatctg catttttttg ccctgagtaa cacagaaatg ggctgcatca
    19081 ttttttacta tattttctgc accagatatt ctgcccctgg ctaaagaagc ttcggcttcg
    19141 gtgtagtatt ggttatcgag tttacgctga atattacttt tatatgcaag accaaattta
    19201 ccgatacttg tttcatcatt atgcacagca caaccagata taagaaaa
//
LOCUS       DAZPCS010000067        18734 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZPCS010000067 DAZPCS010000000
VERSION     DAZPCS010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18734
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.067"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(11..250)
                     /locus_tag="ACHUQ7_002250"
     CDS             complement(11..250)
                     /locus_tag="ACHUQ7_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFR1135646.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(253..972)
                     /gene="sanA"
                     /locus_tag="ACHUQ7_002251"
     CDS             complement(253..972)
                     /gene="sanA"
                     /locus_tag="ACHUQ7_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFR1135647.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(1122..2006)
                     /gene="cdd"
                     /locus_tag="ACHUQ7_002252"
     CDS             complement(1122..2006)
                     /gene="cdd"
                     /locus_tag="ACHUQ7_002252"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFR1135648.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(2136..2831)
                     /locus_tag="ACHUQ7_002253"
     CDS             complement(2136..2831)
                     /locus_tag="ACHUQ7_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFR1135649.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(2828..3226)
                     /locus_tag="ACHUQ7_002254"
     CDS             complement(2828..3226)
                     /locus_tag="ACHUQ7_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFR1135650.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            3465..4412
                     /gene="dusC"
                     /locus_tag="ACHUQ7_002255"
     CDS             3465..4412
                     /gene="dusC"
                     /locus_tag="ACHUQ7_002255"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFR1135651.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGFWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            complement(4783..4866)
                     /gene="yohP"
                     /locus_tag="ACHUQ7_002256"
     CDS             complement(4783..4866)
                     /gene="yohP"
                     /locus_tag="ACHUQ7_002256"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFR1135652.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            5090..6526
                     /gene="mdtQ"
                     /locus_tag="ACHUQ7_002257"
                     /pseudo
     CDS             5090..6526
                     /gene="mdtQ"
                     /locus_tag="ACHUQ7_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
     gene            6618..7340
                     /locus_tag="ACHUQ7_002258"
                     /pseudo
     CDS             6618..7340
                     /locus_tag="ACHUQ7_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013097944.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(7457..8035)
                     /locus_tag="ACHUQ7_002259"
     CDS             complement(7457..8035)
                     /locus_tag="ACHUQ7_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR1135653.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            8205..8792
                     /locus_tag="ACHUQ7_002260"
     CDS             8205..8792
                     /locus_tag="ACHUQ7_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFR1135654.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLTLTVILWGYGYRLF"
     gene            8966..9898
                     /gene="pbpG"
                     /locus_tag="ACHUQ7_002261"
     CDS             8966..9898
                     /gene="pbpG"
                     /locus_tag="ACHUQ7_002261"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFR1135655.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(9936..11651)
                     /gene="dld"
                     /locus_tag="ACHUQ7_002262"
     CDS             complement(9936..11651)
                     /gene="dld"
                     /locus_tag="ACHUQ7_002262"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFR1135656.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            11847..14144
                     /gene="bglX"
                     /locus_tag="ACHUQ7_002263"
     CDS             11847..14144
                     /gene="bglX"
                     /locus_tag="ACHUQ7_002263"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFR1135657.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQGQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPISFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            14396..15313
                     /gene="osmF"
                     /locus_tag="ACHUQ7_002264"
     CDS             14396..15313
                     /gene="osmF"
                     /locus_tag="ACHUQ7_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFR1135658.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVGRGAITSDELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPTFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            15320..16477
                     /gene="yehY"
                     /locus_tag="ACHUQ7_002265"
     CDS             15320..16477
                     /gene="yehY"
                     /locus_tag="ACHUQ7_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFR1135659.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGLHLWQL
                     WPQTIWMLVGVGCAWLTACFVPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGRGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            16470..17396
                     /gene="yehX"
                     /locus_tag="ACHUQ7_002266"
     CDS             16470..17396
                     /gene="yehX"
                     /locus_tag="ACHUQ7_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFR1135660.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVHLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            17401..18132
                     /gene="yehW"
                     /locus_tag="ACHUQ7_002267"
     CDS             17401..18132
                     /gene="yehW"
                     /locus_tag="ACHUQ7_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFR1135661.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQVTLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(18113..18220)
                     /locus_tag="ACHUQ7_002268"
     CDS             complement(18113..18220)
                     /locus_tag="ACHUQ7_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFR1135662.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(18280..18734)
                     /locus_tag="ACHUQ7_002269"
                     /pseudo
     CDS             complement(18280..18734)
                     /locus_tag="ACHUQ7_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
BASE COUNT         4481 a         4726 c         5156 g         4371 t
ORIGIN      
        1 agagaaaata ttaacgatag aaaataagat aaacaatcat gccgatagat aaaattccga
       61 agccaatata tgccaccata taagcgaaga agagtccggg attttttgct cgccagacat
      121 acaccggttc cggtgcattt ttaacccgtt tatcaatcgc gcctgcgcgc cagcctttcc
      181 atgcttcgcg gaaacaaaat atcgggataa ggaaaaaaac ggcaacgaca aaaaactgct
      241 gtacatccat aactactttc cttgtttctt ttgtaattca agtaactgtt cgggtgtaac
      301 ggcgggatac ccctgcgcat cttccggcac ctggtgcata gccggaatag ggaccagcgg
      361 ccctaaaaaa cgcggttcac gtttaaaaat ataaaggtca gccagcgcac cgaaacgggc
      421 ggcaaattcg cgaatacgta ctgacagcat atctttcggt gacggtacgg cataacactg
      481 agcctgaatc cccatatgca gcgcaataaa taatgctcgc tcacagtgga aacgttgggt
      541 gataataatg aaatcattag tatcgaaaac tttgcgtgta cgcacgatgg aatccagcgt
      601 acgaaagcct gcgtaatcga gaacaatatc tgatgggtcg acaccagcag cgattaaatc
      661 tttgcgcatg gtcatcggct cgttataact ttgcaatgcg ttatcgccgc tcagtaatag
      721 ataatttacc ttaccgctgt tataggcatt aatcgctcct tgaatgcggt agcgataata
      781 ctgattaata acgccggtac gataatattt tgctgtgccg agcaccacgc cgacctggcg
      841 gtaggggaga tcctgcaatt cgtcgtagat ataaggcgcg gttttccagc tcatccagcg
      901 atcgaggccg agcacagtca acagcagcaa gccgatcagg actaacaggc tgaggaacac
      961 gcgctttaac atgcaggaga ctcgagctaa ggaaaatttc catcaggcta ctttacccgc
     1021 tggttttgcg caagaaacgg caaactgttt gccgggaatt tcgacaccag gagagggctg
     1081 gcaagccgga caaggcgttc acgccgcatc cggcaccagg cttaagcgag aagcacgcgg
     1141 tcgatactgt gacagccgag agctttcagc gttgcggagg tggcatccca ctgaatcaac
     1201 ggcgcatcgg ctttttctgc cagaaccgcg cgctggatat ccgggtaatc ataacccttg
     1261 agattcaaca gaattaacgc cccctgcaac ggcggcagag tcgggttgaa tgcggcgttt
     1321 tcagcgtagc tgccgctgaa aatacgaccg tctttacatt ccagcgcgac accgcttggt
     1381 gacttactgt aaggcatgtg cgaacggttt gccgctgcaa tcgccgcctg agaaagcgca
     1441 tcaccagtca gcgcatagcc gtgatcctgc tcgtccatca gcagcgtttt aatctccaga
     1501 tctttcggcc caaaggcatc cggcagatag tcacgcagcg cgtgtgcctc gcggcccggc
     1561 aaatgaatac gcagatccag accgctgttc agttcattca taaactgacg gcagtgacca
     1621 caaggcgtat agttaacggt gatggctgca agcgcttttt caccactcaa ccaagcgtgg
     1681 ctgatcgcgc tttgttcggc atgaacggtt tgctgcattg ttgcaccaat aaattccata
     1741 ttggcaccaa aataccaggt tccgctcaca ccgcgcgcaa ttgcgccaac attaaaattc
     1801 gacaatggcg tacgcgcaca ggccgccgcc agcggaagta gtgcgaatgc cagcgcgtct
     1861 tcgtccagtc ccgttgcgct cttcagcgat gagacttgct ccccggtcaa caaagcgggg
     1921 aagtacttgt ctgccagaat aggttccagt gcagattgca agttatccgc aagttgggca
     1981 aaagcggttt gaaaacgtgg atgcatggcg ttgcctcata atcatgtaat gggataacag
     2041 tttacgaacc cgttatggct ttatatgtga tctgaatctc attaattatg caacacgtga
     2101 aacttacatc aaaaatgcgc gacgtatcgc aaattttagc ccattactgc cagaataatc
     2161 gggaaaagga acggtgcgat cagcgaagta attatcccgc ataacaccag cgctagcgaa
     2221 ctaaatgcac cttcctgata atccagctcg gcgcagcgcg ccgtaccgag ggcgtgcgag
     2281 gcagtcccca ttgccagacc gcgcgcagct ttggtacgaa tacgcatcgc attaagcaat
     2341 gtatggccaa ataccgcgcc gaggatgccg acgaaaatca cgcaaactgc gctgattgcc
     2401 ggaataccgc caatactgcc gccaaccgcc attgcaatag gcgtggtgac tgatttcggc
     2461 aggattgacg cggcgatttc cggtgaagcg cccatcaaca atgccacgga agtccccgtc
     2521 accatcgcaa ccacgctgcc gataaaacag atggtgatga tcgatttcca gcgcgcgcgg
     2581 atctggtgta gctgctcata taaaggatag gccagcgcga cgaccgccgg ttgcagcaga
     2641 tcgtttagca cttcgctacc tttaaagtaa ctgtcgtaag agatgccggt cagcattaaa
     2701 aaaggaatga tcaccaccat tgctaccagc aacgggttaa gcaggggaaa cttataccgt
     2761 gccgccagtt tgcgggcggc aaaaaagaca atcaacgtca gcggtaatga ccaccagata
     2821 ttcgccatca ttcttctgat cctttctgac ctacgacttt acgttcaccg tgtaccagtt
     2881 gtgaactcca gctcaccacc agaaaaacca ccagcgtact gacagcacag gaaaccacta
     2941 ccgggccaaa ctgcgcgcgg agcaaatcaa aatattgcat gacgcctacg ccaatcggca
     3001 caaacaatag cgccatatag cgaatcagta cgtagcaccc cggattgacc catttcgccg
     3061 gaagaatttg caaggccagc aggacaaaca ggatcagcat cccgatgatg ctgcccggaa
     3121 tggttaccgg tagcagggaa gcaatgaaaa tgcctgcata caggcaggca taaatcagga
     3181 cgaaagcgcg taaatattgc cagataatgt tcagtgtctt actcataatc aacaccccag
     3241 atgagatgga ttcatcatac aattaattcg taaaacgtgc taccgatcac attaagaaat
     3301 cagcgcttat tttttcgcta cccggtatta cgcaatgagc agcgaactca ggcatacatc
     3361 aagcatagag agggattgca gtttgcagca atccctcttt gcagaccttt aatccgctga
     3421 ccataggcgc taacatagcg cctcattttt ttgcgggtga tgatatgcgt gtgttactgg
     3481 caccgatgga gggagtgctt gactctctgg tgcgtgaatt gctgaccgaa gttaacgact
     3541 acgatctgtg catcaccgag tttgtccgcg tggtggatca actgctgccg gtaaaagtct
     3601 ttcatcgcat ttgccctgag ctacaaaacg ccagccggac accatctggt acgctggtgc
     3661 gcgtgcagtt gttaggtcag ttcccacaat ggctggcaga gaacgccgcc cgtgcggtgg
     3721 agttaggttt ctggggcgtg gatctcaatt gcggctgccc gtcgaaaacg gttaacggta
     3781 gcggcggcgg ggcgacgtta ctcaaagatc ctgaactcat ctaccagggg gcaaaagcga
     3841 tgcgtgaagc tgtaccggcg catttgcctg tcagcgtgaa agtgcgtctg ggctgggaca
     3901 gcggtgagaa gaaatttgaa atcgccgatg cggttcaaca ggctggcgct acggagctgg
     3961 tggtgcatgg gcggacgaaa gagcagggtt accgcgcgga gcatattgac tggcaggcga
     4021 ttggcgagat tcgccagcgg ctgaatattc cggtgattgc caacggtgaa atctgggact
     4081 ggcagagcgc gcaacaatgc atggcgatca gcggctgcga cgcagtgatg attggtcgcg
     4141 gggcgctcaa tattcccaac ctgagccggg tggtaaaata taacgaaccg cgaatgccgt
     4201 ggccggaggt ggttgctttg ctgcaaaaat atacccgtct ggaaaagcag ggcgataccg
     4261 ggttatatca cgttgcgcgg attaaacagt ggttgagtta tttgcgtaaa gaatacgatg
     4321 aagcaacgga attatttcag catgttcggg tgttgaataa ctcccctgat attgcaaggg
     4381 ctattcaggc aattgatatc gagaaactct aaaaaccata gtgatgtttt tgacaatgtg
     4441 ttagctcttt atcaataggc cgcacggttg tttggtggcg tcaaaatcgc cgaggcgttc
     4501 cctgaaggcc ggggcagccc acatggatgt gggctgaggg cgcgttttac agggatgtta
     4561 cctcgcgccc gacccggtag ccgtaaggga taagccgagg gcaccgcgca gcggcgattt
     4621 tgttcgccag agcccggggg ggcagggggc ggcggcgatt ggtcgccccc tgcgcgctcc
     4681 ttgcgccagt gattatatga agcaatgcac atgacaggag cgcaacagga tgagagactg
     4741 aaatattctc gttattttcg gatctgatga cattaattaa ttttaaaata tcatcttaaa
     4801 tacgccagtc accaccagta gcccaatcag gaaaataatc aatacagccc agagtataat
     4861 tttcattggc gttcctttat ttgtgagtgc tgaataagta taggaaaaaa cgaagccctt
     4921 tgccgttatc cataaacgat taagataaat cggaagatat catttactta gtctatattt
     4981 ttctgggtat gtcgttttat tgctactgat gtattgccac gcgaaactaa aaaatatatc
     5041 gcaacacacg gtaatctccg cgcaccgttt cttacctgat tattccccca tgaatcgtga
     5101 ttccttttat ccagccatcg cctgttttcc gctgttactg atgctggccg ggtgtgcgcc
     5161 tatgcatgaa acccgccagg cgttaagcca gcaaacgccc gctgcacaag ttgacaccgc
     5221 attacccacg gcgctgaaaa atggttggcc agacagccaa tggtggctgg agtatcacga
     5281 taatcagctc acttcattaa ttaacaatgc actacagaac gcaccggata tgcaggtggc
     5341 tgaacagcgc atttagctag ctgaagccca ggcgaaggca gttgccactc aggatggtcc
     5401 gcagatcgac ttttcggcgg atatggagcg gcaaaaaatg tcggcagaag ggctaatggg
     5461 gccgtttgct ctgaacgatc cggccgcagg tacgaccggt ccgtggtaca ccaacggaac
     5521 ttttggctta acggcgggct ggcatctcga tatctgggga aagaatcggg cggaggttat
     5581 tgcccgcctg ggtacggtta aagcacgggc ggcggaacgc gagcaaaccc gccaattgct
     5641 ggctggcagc gtagcccgcc tgtactggga gtggcaaacc caggcggcgt taaacacggt
     5701 cttgcagcaa atagaaaaag agcagaacac cattatcgcg accgatcgcc agctatatca
     5761 gaacgggatt acttcttcag ttgacggtgt ggaaaccgat attaatgcca gcaaaacccg
     5821 gcagcagctc aacgatgtcg cggggaaaat gaaaattatt gaggcacggt taaatgcact
     5881 cacaaatcac cagactaagt cattgaagct taaaccggtc gcgttgccga aagtggcaag
     5941 ccagcttcct gatgaactgg ggtactcctt actggcccgg cgggcagatt tgcaggcggc
     6001 gcactggtac gttgagtcat cgttaagcac tattgatgcg gcaaaagcgg cattttatcc
     6061 tgacatcaac ctgatggcct tcctgcaaca ggatgcgttg cacttaagcg atctgttccg
     6121 tcattccgcg cagcaaatgg gcgttacggc aggcctgacg ctacctattt tcgatagtgg
     6181 tcgtcttaac gccaatctcg atatcgcaaa agccgaaagc aacttgtcta tcgccagcta
     6241 caacaaagcg gtggttgaag cagtgaatga cgtggcgcgg gcagccagtc aggttcagac
     6301 actggcggag aaaaaccagc atcaggcgca aattgagcgc gatgccttgc gtgtggtagg
     6361 tcttgcgcag gcccgcttta acgcgggcat cattgctggt tcccgcgtca gcgaagccag
     6421 aatcccggcg ctgcgtgagc gggccaatgg cctgttattg caagggcagt ggctggatgc
     6481 ctccattcga ctcaccggtg cgctgggcgg gggttacaaa cgctgatgat atgcgccttc
     6541 tatacttaac gtttattcag cgttaagtgg agaactcgat ggcataggtc gcgataatta
     6601 ccgcttccga ttcggggatc ggcaaagagt gcgcgttatt actggcgcag caggggtttg
     6661 atattggcat tacctggcac tcagatgaag aaggggcaaa agataccgcg cgtgaggtgg
     6721 ttacccacgg cgtacgtgcg gagatcgtgc aactggatct cggcaatctg ccagaaggtg
     6781 cgcaggcgct ggagacactc atccagcgat ttgggcgtat tgatgtgctg gtgaataatg
     6841 cgggtgcaat gaccaaagcg ccgtttcttg atatggcttt tgatgagtgg cgcaagattt
     6901 ttaccgttga tgttgatggt gcatttttat gctcgcaaat tgcggctcgt cagatggtga
     6961 aataagggca gggcggtcgc atcatcaaca ttacgtcggt acatgaacat acgccgctgc
     7021 cggatgccag cgcctacaca gccgctaaac atgcgctcgg cgggttaacc aaaacgatgg
     7081 cgctggagtt ggtcaggcat aagattttgg tgaacgcagt cgcgcctggg gcgatagcca
     7141 cgccaatgaa cagcatggat gatagcgacg tgaagcccga cgcggagcct tcgattcctc
     7201 tacggcgttt tggcgcaacg catgagattg ccagcctggt ggtgtggctt tgttcggagg
     7261 gcgcaaatta cacgacaggg cagtcgttga tagtggatgg cggctttatg ttggcgaatc
     7321 cgcagttcaa cccagaatag cgcagtgagg tgcgtggata tttcaatggg ttaggccaga
     7381 taagacacca gcgtcgcatc aggcatggaa cacccggtgc cggatgtgga gtgaatgctt
     7441 atctgtggca actgctttac gcctgattat ccggcttttt cttcccgcgc cgtttcagcc
     7501 accagcgcac gcccaccacc agaaccacga ccagaatcaa ccagacccag tgcttcaaat
     7561 gctggtcgag attgtgcaac cacggcgcaa tcacctgacc accagcgtaa ccaatagtgg
     7621 taaaaatcaa cgcccaggca aatgcgccga gaatattcag cggcagaaag attttcggcg
     7681 gcagctggct ggcaccaatc agcgtcgggc caatcacccg aaagccatac ataaagcgcg
     7741 taccaatgac aaacagatac ggatggcgtt ggataagttt ctgtgcccgc tcaattttat
     7801 cctgatgttt cgagaaacgg cgtaacagct tgccgccaaa ccgccgcccg cacagataga
     7861 gcacctggtc gccaatcatg ccaccaagcg ccacagaaag taccaccagt gggaacttta
     7921 atagcccctg atgtgccgca acgcctccca gcaaagtcac ggtttcacct tccgccaggc
     7981 taccgataac cagcgcggca taaccatatt gtgagatgag tgtattgaga tccataggcc
     8041 agtttgtctc cctttggttg cctgtaagca tacaccctgt gaatcattca cgctgaaaaa
     8101 ggacatacat cagacggtgc aaattacatg gatgaacaaa aaataatcac aagctggctt
     8161 atacttgaac tgtcaggttc atgaactcac aacaaggggg cggtatgagc catgtctggg
     8221 gactgttttc ccatcccgat cgtgaaatgc aggtgattaa tcgcgaaaac gaaacgattt
     8281 ctcaccatta cacccaccac gttttgctga tggcggcgat tccggtgatt tgcgccttca
     8341 ttggcactac acagattggc tggaattttg gcgatggcac tatcctgaag ttatcctggt
     8401 ttaccggact ggcgctggct gtcttgtttt acggcgttat gctggctggg gttgcggtga
     8461 tggggcgggt catctggtgg atggcgcgca attacccgca gcgtccgtct cttgcgcact
     8521 gcatggtctt cgcgggctac gttgcaactc cgctgttttt aagcggtctg gtggcgcttt
     8581 atccactggt ctggttgtgt gcgctggtcg gcacggtggc gctgttttac accggctacc
     8641 tgctttacct tggtattccg agcttcctga atatcaacaa agaggaaggc cttagcttct
     8701 ccagttcgac gctggcgatt ggcgtgctgg tactggaagt gctgctcacc ctaaccgtta
     8761 tactatgggg ctacggttat cgtttgttct agaaagttct ttgacgttgc attgctggcg
     8821 taaatgtcag caatgcagca tttactcacg attctccttt gacgatcgca ctttttgctc
     8881 gttatgatgg cgtcgctagc ctcagtaaat ccttactccg gcggtgcgca acccgtgcgc
     8941 gtgaaccact atctgaatgc tcatcatgcc gaaatttcga gtttctttat ttagcttggc
     9001 cctgatgctg gctgtgcctt ttgcaccgca ggccgttgcg aaaacggcag ccgctaccac
     9061 cgcttcacaa ccggaaattg cctccggtag cgcgatgatt gttgatctga ataccaacaa
     9121 agtgatctat tcgaaccacc cggatctggt gcgtccgatt gcgtctatca gcaaattaat
     9181 gaccgcgatg gttgtgctgg atgcacgact gccgctggat gaaaaactaa aagtggatat
     9241 cagccagacg ccggagatga aaggggtcta ttcgcgcgta cgactgaata gcgaaatcag
     9301 ccgtaaagat atgctgttgc tggcgctgat gtcttcagaa aaccgcgcgg cggcaagcct
     9361 tgcgcaccat tatcccggtg gttacaaagc ctttattaag gcaatgaatg cgaaagcgaa
     9421 atcgctcgga atgaacaaca cgcgctttgt tgaacctacc ggattgtcgg tgcataacgt
     9481 ttcaactgcc cgtgacttaa ccaaactgct cattgccagc aaacaatatc cgttgatcgg
     9541 gcagttaagt actacccggg aagatatggc aaccttctct aacccgacgt atacgctgcc
     9601 gttccgcaat actaatcatc tggtgtatcg cgataactgg aatattcagt taaccaaaac
     9661 cggctttacc aatgcggcgg gccattgtct ggtgatgcgt acggttatca ataataaacc
     9721 ggtggcgctg gtagtgatgg acgcgtttgg caaatatacc cattttgccg atgccagccg
     9781 cctgcgtacc tggattgaaa ccggtaaagt gatgcctgtg ccggcagcag cgttgagcta
     9841 taaaaaacaa aaagccgccc aaatggcggc ggcggggcag acggcacaga acgattaagt
     9901 gaattcggat ggcgatactc tgccatccgt aatttttact ccacttcctg ccagtttttc
     9961 cgtttactgg ttttaccgat ccccggattc atgctattgg tcggatcatt ctcgcgatag
    10021 aacttctgca acgtctccgg tgctttatac aaatgaccga cgttatgctc ggcagggtac
    10081 tgcgcgccgc gctgctgtag cagttccagc atctgttctt ttaacgcatg cacatccacg
    10141 ccttttttca ctatgtaatc ctgatggaag acatagcaca taaaatggcc gtaatagagc
    10201 ttgtgcacca gctggctgtc gatctccggc ggtaaatgct cataccactc ggtgtcgtta
    10261 cgccgcagag cgatatccag tgccagaatg tcttcgactt catcggaatg caccgcctga
    10321 taacgaattg ctgcgcccgc agcggcgaaa cggtgtaaaa acgctttgct accttcctcc
    10381 ggcgtacaga caaagaaatc gccctcggcc tgtttgaaat aatccaccag ccacgatttg
    10441 gcttcaccga cgccatcgcc tgccattttt aacagcagat gatgctcgta tttatcgcgc
    10501 cagtttttca tgcgcggcgg taaatggctg gggaacaggt gaccgaattt ttgcattgca
    10561 cggtcagtaa aatgcggacg gaagaatttc actttctcca gcatcgcatc ggtgcgtccc
    10621 ttgagattaa agaagaacgg catcttgtcg gtgcctaact tatcaatcat caggaaggtg
    10681 tctttgccgt atttttccgc aatatcgtag atatcccggt gcatatattc cccggcaacc
    10741 ggcagatttt cgaagttagc cagaatatga cggcggattt cggtcagcac ttccggctgg
    10801 ttggtgccga tataaaacac ctgctgattt ttttccgctt cgaaggtatc aagacgtacc
    10861 gcaaagacgg ccagcttccc ggcgcaacca gaagattcaa ataaccgatc cggatcggcg
    10921 ttataacgtg cgggcgtgtc ggcttcaata tcacgaacgc ggtggacata gtcataatcg
    10981 tgggcgtgac gaccatcgtg acgcacatcg tcatctttga tgcgatcgtc atccagcttg
    11041 ctaaggatct gctccggcgt ttcgcccaga tcaatcccca gatggttcac cagcgtcagt
    11101 ttgccatctt catctatacg cgcgaataac gacatttcgg tatacgccgg gccacgttgc
    11161 accagcgagc cgccggagtt gttacaaata ccgccgatga ccgatgcgcc tatacacgat
    11221 gatccaatca ccgaatgcgg ttcgcgtccc agcggtttga gggctttttc cagcgaatag
    11281 agcgtggtgc ccggataggc cagcacctgt tcgcccttgc cgagaacgtg cagcttgtcg
    11341 agacgcaggg tgctgatgat cacgatatcg cgatcatagt cgttaccgtt tggcgtcgat
    11401 ccttcggtca ggcctgtatt ggcagcctgc atcagaataa ttttgtcggc ggtgacgcag
    11461 gctttcagca cccgccacaa ttccagtagt gagccaggga aaacgacagc cagcgcgtcg
    11521 ccctgaccag aacggaagcc cttgcgatag cgggccgttt ttgcgggatc ggtgagcagg
    11581 tgtgaatgac cgaccagacg ggcaagttca ttcaaaaagg ctttattatc agttgttgtc
    11641 atggaagaca ttttccactc cttgtggtgg cgaaaaaata tcacaaacta gaatagcgcc
    11701 ttgtatgaca attcagcgag tgaatatttt gaaaattcag acaataaact gagcattctt
    11761 tttctctctc catcatgctt atggcaaact acggtttttg caccattttc gctgccacgt
    11821 cgggcaacaa aggaagaaaa atccatatga aatggctatg ttcagtagga atcgcggtga
    11881 gtctggtcct gcagccagca ctggcggatg atttattcgg taaccatcca ttaacgcctg
    11941 aagcgcggga tgcgttcgtc accgatctgc tcaagaaaat gacggttgat gagaaaattg
    12001 gtcagctgcg cttaatcagc gtaggcccgg ataatccgaa agaggcgatc cgcgagatga
    12061 tcaaagacgg tcaggttggg gcgattttca acaccgtaac ccgtcaggat atccgcgcca
    12121 tgcaggatca ggtgatggaa ttaagccgcc tgaaaattcc tcttttcttt gcttacgacg
    12181 tgctgcacgg tcagcgcacg gtgttcccga ttagcctcgg tctggcctcg tcttttaacc
    12241 tcgatgcggt gaaaacggtc gggcgtgtct ctgcttatga agcggcagat gatggcctga
    12301 atatgacctg ggcaccgatg gtcgatgtct cgcgcgatcc gcgctgggga cgtgcttccg
    12361 aaggttttgg cgaagatacg tatctcacct caataatggg taaaaccatg gtggaagcga
    12421 tgcagggtaa aagcccggca gatcgttact cggtgatgac cagcgtcaaa cactttgccg
    12481 catacggcgc ggtagaaggc ggtaaagagt acaacaccgt cgatatgagt ccgcagcgcc
    12541 tgtttaatga ttatatgccg ccgtacaaag cggggctgga cgcaggcagc ggcgcggtga
    12601 tggtggcgct gaactcgctg aacggcacgc cagccacctc cgattcctgg ctgctgaaag
    12661 atgttctgcg cgaccagtgg ggctttaaag gcatcaccgt ttccgatcac ggtgcaatca
    12721 aagagctgat taaacatggc acggcggcag acccggaaga tgcggtgcgc gtggcgctga
    12781 aatccggaat caacatgagt atgagcgacg agtactactc gaagtatctg cctgggttga
    12841 ttaaatccgg caaagtgacc atggcagagc tggacgatgc tgcccgccat gtactgaacg
    12901 ttaaatatga tatggggttg tttaacgacc catacagcca tttggggccg aaagagtctg
    12961 acccggtgga taccaatgcc gaaagccgcc tgcaccgtaa agaagcgcgt gaagtggcgc
    13021 gcgaaagcct ggtgttgctg aaaaaccgtc tcgaaacgtt accgctgaaa aaatcggcca
    13081 ccattgcggt ggttgggcca ctggcggaca gtaaacgtga cgtgatgggc agctggtccg
    13141 cagccggtgt tgccgatcaa tccgtgaccg tactgaccgg gattaaaaat tccgtcggtg
    13201 aaaacggtaa agtgctgtat gccaaagggg cgaacgttac cagtgacaaa ggcattatcg
    13261 atttcctgaa tcagtatgaa gaagcggtca aagtcgatcc gcgttcgccg caagagatga
    13321 ttgatgaagc ggtgcagacg gcgaaacaat ctgatgtggt ggtggctgta gtcggtgaag
    13381 cacaggggat ggcgcacgaa gcctccagcc ggaccgatat cactattccg caaggccaac
    13441 gtgacttgat tgcggcgctg aaagccaccg gtaaaccgct ggtgctggtg ctgatgaacg
    13501 ggcgtccgct ggcgctggtg aaagaagatc agcaggctga tgcgattctg gaaacctggt
    13561 ttgcggggac tgaaggcggt aatgcaattg ccgatgtatt gtttggcgat tacaacccgt
    13621 ccggcaagct gccaatttcc ttcccgcgtt ctgtcgggca gatcccggtg tactacagcc
    13681 atctgaacac cggtcgtccg tataatgccg acaagccgaa caaatatact tcgcgttatt
    13741 ttgatgaagc taacggagcg ctttatccgt tcggctatgg tctgagctat accactttca
    13801 ccgtctctga tgtgaaactt tctgcgccga ccatgaagcg tgacggcaaa gtgacggcca
    13861 gcgtgcaggt gacgaacacc ggtaagcgcg agggggccac ggtagtgcag atgtacttgc
    13921 aggatgtgac ggcttccatg agtcgccctg tgaaacagct gaaaggcttt gagaaaatca
    13981 ccctgaaacc gggcgaaact cagactgtca gcttcccgat cgatattgag gcgctgaagt
    14041 tctggaatca acagatgaaa tatgacgccg agcctggcaa gttcaatgta tttatcggca
    14101 ctgattccgc acgcgttaag aaaggcgagt ttgagttgct gtaatgcttt agtaagggcg
    14161 cgacgtttgc gccctttgcc tgatgttcat atcttaactt gctgaatttt cgccctttgt
    14221 aggccggata aggcgctcgc gccgcatccg gcaggaaacg ccggaccgaa tatgcctctt
    14281 aagtatttat ctgattctgc aactgaatcc ttccgctcag gctaactctg tcattatcaa
    14341 ctatgctttt ctcttaattc gctgaaaaca acagtaaaag aatgaggaaa gcatcatgcc
    14401 actcttaaag ctctgggcag gttcactggt tatgttggca gccgtgagcc tgccgctaca
    14461 ggcggcttcc cccgttaaag tcggttcaaa aatcgatacc gaaggcgcgc tgctcggcaa
    14521 catcatcttg caggtactcg aaagccacgg cgtaccgacg gtgaataaag tgcaactcgg
    14581 aacgactcct gtggggcgcg gggcgattac ttccgatgaa ctggatatct atccggaata
    14641 taccggcaat ggcgctttct tctttaaaga tgaaaacgat gcagcgtgga aaaatgccgg
    14701 gcagggctac gagaaagtca aaaagctcga tgcggagcac aacaagttaa tctggctgac
    14761 gcccgcgcct gcaaataaca cctggactat cgccgtgcgt caggatgtgg cagagaaaaa
    14821 caaactcact tcgcttgccg acctgagtcg ttatctgcaa gagggcggca ccttcaaact
    14881 ggcagcctcg gcagagttta tcgaacgcgc cgatgcgtta cccacgtttg aaaaagccta
    14941 tggctttaag ctcggtcagg atcagttgct gtcactggct ggtggcgaca cggcggtgac
    15001 gattaaagcc gctgcccagc aaacctctgg cgttaatgcc gcaatggctt acggcactga
    15061 cggtccggtc gcggcgctgg ggctgcaaac cttaagcgat ccgcaaggcg tgcaacctat
    15121 ctacgcgcct gcaccagtgg tgcgtgaatc ggtgttgaag gagtatccgc aaatggcaca
    15181 gtggctacag ccagtcttcg ccagcctcga tgcaaaaaca ttgcagcaac tgaatgccag
    15241 cattgcagtg gaaggactgg atgccaaaaa agtggctgcc gactacctga aacaaaaagg
    15301 gtgggcgaag taatttcccg tgacttatct acgtattaat cctgttctgg cgctgctgtt
    15361 gttgctgacg gcaatcgcag cggcgctgcc gtttatcagt tacgcgccta atcgtttagt
    15421 ctcgggtgaa gggcttcatc tctggcaact gtggccgcaa acgatctgga tgctggtggg
    15481 cgttggttgc gcctggctga cagcctgttt tgtccccgct aaaaaaggca gcatttttgc
    15541 actcattctg gcgcaattcg tcttcgtatt gttggtgtgg ggagctggaa aggcggcgac
    15601 ccaactggcg caaaatggca gtgcgctggc gcgtaccagc ctcggcagag gtttctggct
    15661 ggctgctgcg ctggcattgc tggcctgtag cgatgccatc cgccgaatct ccacgcatcc
    15721 gctgtggcgc tggttgttgc atatgcagat tgccattatt ccgctgtggt tgctgtactc
    15781 cggcacgctt aacgatctct cactaatgaa agaatacgcc aaccgtcagg atgtgtttga
    15841 cgacgcgctg gcacaacatc tgacgttgct gtttggtgcg gtgctgcctg cgttagtgat
    15901 tggtgtgccg ttgggcatct ggtgctactt ttccactgcg cggcaggggg caattttttc
    15961 tctgctcaat gtcattcaga ccgtgccttc ggtggcgctc tttggcctgt tgattgcgcc
    16021 gcttgccgcg ctggttacgg cctttccgtg gctggggaag ctcggcatag caggaaccgg
    16081 aatgacaccc gcactgattg cgctggtgct ctatgccttg ctgccgctgg tgcgaggcgt
    16141 ggtagtcggt ttgaaccaga tcccgcgcga tgtgctggag agcgccagag cgatggggat
    16201 gagcggggcg cagcgtttcc tgcatgttca gttaccgctg gcgttaccgg tatttctgcg
    16261 cagcctgcgg gtggtgatgg tgcaaactgt aggtatggcg gtgattgcgg cgttaatcgg
    16321 cgcaggcggt tttggtgcgc tggttttcca ggggctgcta agcagcgcca ttgatttagt
    16381 gttgctgggg gtgatcccgg taattgttct ggcggtgctt accgacgcgc tgttcgattt
    16441 gcttatcgca ctgctgaagg tgaaacgtaa tgattgaatt tagccatgtc agcaaactgt
    16501 tcggcgcaca aaaagccgtt aacgatctca atctcaattt tcaggaaggg agtttttcgg
    16561 tgctgattgg cacatctggc tccggcaaat ccaccaccct gaaaatgatt aaccgcctgg
    16621 tggagcatga cagcggagtg atccgctttg ccggagaaga aattcgctcg ctgccagtac
    16681 tggagttgcg ccgccggatg ggctatgcca ttcaatctat tggcctgttc ccccactgga
    16741 gcgtggcgca aaacatcgct accgtgccgc aattacaaaa atggtcgcgg gcgcggattg
    16801 acgatcgtat cgacgaatta atggcgctac tggggctgga gtcaaatttg cgtgagcgtt
    16861 atccgcatca gctttccggt ggtcagcagc aacgtgtggg agtggcgcgt gcactggctg
    16921 ccgatccgca agtcttactg atggatgaac cttttggcgc actggacccg gtaacgcgcg
    16981 gcgcgttgca acaagagatg acgcgcattc accgtttgct ggggcgcact attgtgctgg
    17041 tcactcatga tattgatgag gcgctacggc tggcagaaca tctggtattg atggatcacg
    17101 gtgaagtggt gcagcagggg aatccgctga cgatgctgac tcgtccggcg aatgattttg
    17161 tccgccagtt ttttggacgt agtgaactgg gggtgcacct gctttcgtta cgtagcgtgg
    17221 cggattacgt gcgtcgcgaa gaacgggcag aaggtgaggc actggcagaa gagatgacgc
    17281 tacgcgatgc gctctccctg tttgtcgcgc ggggatgcga ggtgctgccg gtggtgaaca
    17341 cgcagggcca gccttgcggc acgctgcatt ttcaggatct gctggtggag gcgtaagcgt
    17401 atgaagatgt tgcgcgatcc gctgttctgg ctcattgctc tttttgtggc gctgattttc
    17461 tggctgcctt acagccagcc gctgtttgct gccttgttcc cacaactgcc acgacccgtt
    17521 tatcagcaag aaagttttgc agctctggca ctggctcatt tctggctggt gggaatttcg
    17581 agtttgtttg cggtgatcat tggcacaggt gccggaattg ctgttactcg cccgtggggc
    17641 gcggaatttc gcccactggt ggaaactatt gccgccgttg gacagacttt tccgcctgtc
    17701 gcagtgctgg cgattgccgt tccggtgatc ggctttggtc tgcaaccagc gattatcgcc
    17761 ttgatccttt acggtgtgct gcccatcctg caggtgacac ttgccgggct gggagcgatt
    17821 gatcccagcg tgacagaagt tgcgaaaggt atgggaatga gtcgaggtca gcgactgcgt
    17881 aaggtcgagc taccgctggc ggctccggtg attctggcgg gcgtgcgaac ttcggtgatt
    17941 atcaacattg gtacggcgac gatcgcctca acggtagggg ccagtacgct gggtacgccc
    18001 atcatcatcg ggcttagcgg atttaatacc gcgtatgtga tccagggggc gttactggtg
    18061 gcactggcgg cgatcatcgc agaccgcctg tttgaaaggc tggtgcaggc gcttagccag
    18121 cacgcaaaat aaaggtataa cctgcgagca tgacgccacc aattccgcct aacgccataa
    18181 acaggaacag ggcgatgacc ccaattttag ctatgcgcat aatgcactcc ttatgttaac
    18241 gaaaggattg tacagtaaag cgcatttgtt aacgaatcat taaataccga gtgggaaaat
    18301 atcatggcct tgttcttgcc aactggtgag ttgctgctgt tgggcggagg ttcgattttc
    18361 accgcaccac accagcaatg tacggccttc gaatagttca gggcgtagtt gattgagcga
    18421 gtgggcgagc gaggacatca atgcgccatc cttgttgact ggcaatccag ccctccagcc
    18481 acagacgggt ggtatcctga atattccagc caaccaccag cgcatcttta ccctgttttt
    18541 tacgtgccga agccagacaa atggcgatgt agttgatcag tacgccgtcg aggatcgcca
    18601 gcagcgcctg gagagtcggt tgttggcact gaagccgtcg gcgcagagga ataaacagat
    18661 gtgtggtgag tgtctgggcg gggtaatcct gaccgcgctc tttgatccac gttcgcaggc
    18721 tatgtagatt gccg
//
LOCUS       DAZPCS010000068        18678 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZPCS010000068 DAZPCS010000000
VERSION     DAZPCS010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18678
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.068"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            376..579
                     /locus_tag="ACHUQ7_002270"
                     /pseudo
     CDS             376..579
                     /locus_tag="ACHUQ7_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
     gene            598..3984
                     /locus_tag="ACHUQ7_002271"
     CDS             598..3984
                     /locus_tag="ACHUQ7_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFR1135663.1"
                     /translation="MEEAFWAARQGDALLHTSFMADVLGAVLEVAANVVIDALIVGTT
                     SLLATSVGITMGCTAVVLTGFVAGVAMVYTGVTEKVSEACSALANMLFPPQIEGYILT
                     GSGDTWINSKPAARAAATAASRQDIEAQEAQAKAEQEEAQRQEDARTFRDVAGEYLEM
                     AGAIALAVNPVTGPVLMSRALHDQLSTEEGRSEMVDGVTHFVSELWQPTVASAAPGST
                     PTPDDKIDCHKHPSSLTQFLAQKKAAFLDAPLGTALNLLNPGGMLETAFQGVNAVIGS
                     ISNLFKGDDEPPAAEYIAEGARDVRINSQPATRSGARCTCEARVVNEPGNGAFVSPDV
                     RIGGPLLVVRDIRSGRSQITLVATVALMFLRPGKMLSKIACFAASVGMSMLTQKATSA
                     LPHPVNAATGAKYLADDDDFDFSLPGHFPLDWQRVYSSRDERTEGMFGQGWSVMYEVS
                     LVCTPGSADENCMTFVSGMGRRLDMEAVLPGGGFYSPGEGLAVRRGEQGHWLISSDDG
                     QCFLFEADPHHPQRQRLKMLGDRNSNCLNLYYDDRGRIVEISGEQQRPCIRLYYELAA
                     HPRRVTQIYQHFPETAPLLLRRYSYDEAGHLNGVYDSTGHLLREFAYDENHCMTLHRQ
                     PGGEGYYYQWGWYEGPDDAAWRVTGHHTDSGEQYRLAWDLAQRRLCVTDGLGRTRYHQ
                     WDAQNQVTAYQDEAGQVTTFRWSDEERLLLGMTDPQGGKWRYVYDLQGHITETHDPLG
                     RVEQAQWHPVWHQPETEVDAAGNLLGRRAGERATAENSVVPFNRLMSYRGVHYRYDEF
                     GRAVEKEGRSGTQSYRYDAEHRMVEVTTARGTYRYVYDALGRRTEKQHISPDGKPYNR
                     TKFLWDGMRLAQESRPEGTGSLYIYRDQGSYEPLARVDKAGKEGPNRILYFHTDVNGA
                     PEEMTDSDGKIVWETGYQVWGNTIQEKDHGGVEQNLRYQGQYLDRETGLHYNLHRYYD
                     PDVGRFMVTDPIGLRGGLNLYSYAPNPLKYADPLGLTPCAVSNQKANRLLDSSETKVT
                     VRSRSDAEQLFMDRYLGHNYKNMTGESGPSTKNLMEYLTENKTKAGSYHWDDIKDPSV
                     TKPSYRVSGHGPGNPDGDLPHLQVHQHGGSVRHIFFPWET"
     gene            3987..4367
                     /locus_tag="ACHUQ7_002272"
     CDS             3987..4367
                     /locus_tag="ACHUQ7_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1135664.1"
                     /translation="MNNLNTLLSGKVLLKEISPLMPGYRTWIEISLIDELKPSYPFRL
                     DEYAMIGHSPYANECNKDEAKFKLRISSFLASDIDNEYDPSYDYVGKYEIIASLNELK
                     TRLSTLHVNLEQFINSSEDDEYPL"
     gene            complement(4422..4709)
                     /locus_tag="ACHUQ7_002273"
     CDS             complement(4422..4709)
                     /locus_tag="ACHUQ7_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
                     /protein_id="HFR1135665.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGIARYYSQHPS
                     LHLKGDWLKEAGFDTGRGVTVKISEGGLTIIADSDEVQELRDGMFSVLNES"
     gene            4838..5347
                     /locus_tag="ACHUQ7_002274"
     CDS             4838..5347
                     /locus_tag="ACHUQ7_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFR1135666.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            5344..6762
                     /gene="phrB"
                     /locus_tag="ACHUQ7_002275"
     CDS             5344..6762
                     /gene="phrB"
                     /locus_tag="ACHUQ7_002275"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFR1135667.1"
                     /translation="MTTHLAWFRQDLRLHDNLALAAACRNSSARVLALYIATPRKWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCEN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIIDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(6912..8393)
                     /gene="dtpD"
                     /locus_tag="ACHUQ7_002276"
     CDS             complement(6912..8393)
                     /gene="dtpD"
                     /locus_tag="ACHUQ7_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFR1135668.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            8664..9407
                     /gene="ybgI"
                     /locus_tag="ACHUQ7_002277"
     CDS             8664..9407
                     /gene="ybgI"
                     /locus_tag="ACHUQ7_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFR1135669.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            9430..10086
                     /gene="pxpB"
                     /locus_tag="ACHUQ7_002278"
     CDS             9430..10086
                     /gene="pxpB"
                     /locus_tag="ACHUQ7_002278"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFR1135670.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            10080..11012
                     /gene="pxpC"
                     /locus_tag="ACHUQ7_002279"
                     /pseudo
     CDS             10080..11012
                     /gene="pxpC"
                     /locus_tag="ACHUQ7_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
     gene            11002..11736
                     /gene="pxpA"
                     /locus_tag="ACHUQ7_002280"
     CDS             11002..11736
                     /gene="pxpA"
                     /locus_tag="ACHUQ7_002280"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFR1135671.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            11772..12563
                     /gene="nei"
                     /locus_tag="ACHUQ7_002281"
     CDS             11772..12563
                     /gene="nei"
                     /locus_tag="ACHUQ7_002281"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFR1135672.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(12560..13606)
                     /locus_tag="ACHUQ7_002282"
     CDS             complement(12560..13606)
                     /locus_tag="ACHUQ7_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFR1135673.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(13758..14834)
                     /locus_tag="ACHUQ7_002283"
                     /pseudo
     CDS             complement(13758..14834)
                     /locus_tag="ACHUQ7_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            complement(14831..15562)
                     /locus_tag="ACHUQ7_002284"
     CDS             complement(14831..15562)
                     /locus_tag="ACHUQ7_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR1135674.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            complement(15577..18036)
                     /locus_tag="ACHUQ7_002285"
     CDS             complement(15577..18036)
                     /locus_tag="ACHUQ7_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR1135675.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNEQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSPNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKIPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DGNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            complement(18087..18653)
                     /locus_tag="ACHUQ7_002286"
     CDS             complement(18087..18653)
                     /locus_tag="ACHUQ7_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1135676.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLLLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
BASE COUNT         4617 a         4474 c         5155 g         4432 t
ORIGIN      
        1 tgttgttaat aaaaagctta tagatttata gttgataatt aaaacgcatc cgatatttca
       61 gctttttcta tgcaagatac aattttataa ggtgtccatt tttatatgga ggcggtagcg
      121 tgtctgttac tagtggtttt atttgcatag tgaatatatt gggattgtta aatacataac
      181 tatgtatccg ctctggtcgt tgtaaatatt gctaattgct taatattatt agtgattatg
      241 actggaaaga taaagtgtgc tgtaaagcgg tgatgggtat tacattcatg attaaaaaaa
      301 cagaacctgt caataaataa ttcctttatt aaaacggact atttacatta tgacggaata
      361 ttatctcaac gaggcggttt ggaaatacag agccagtttc tccggggtaa cgagcaggtg
      421 taccagtgtc aggtggcatt tgtattgccg ggtgagcggg tcatgatggc cttcacctac
      481 gccagaacca caccgctgac cccggcagat atgacgcgct gggctgaaat taaaaagaat
      541 ctgcgcttca ggatgcggca ggaagtcagg acgaattaat catacaggga acgggacatg
      601 gaagaggcat tctgggcggc aagacagggt gatgccctgc tgcatacgtc gtttatggca
      661 gatgtgctgg gggcggtgct ggaggtggcc gcaaatgtcg ttattgatgc cctcattgtc
      721 ggcacaacct cgttactggc gacgtcagtg gggatcacga tggggtgcac ggccgtcgtg
      781 ctgaccggtt ttgtggcggg tgtggcgatg gtgtacaccg gcgtgacaga aaaggtcagc
      841 gaagcctgtt cagccctcgc aaatatgctt ttcccgcctc agattgaagg ttacatcctc
      901 accggctctg gtgacacatg gatcaacagc aaacctgccg cgcgggcagc ggcgactgcg
      961 gcatcgcggc aggacataga ggcgcaggaa gcgcaggcga aggcagaaca ggaagaagcg
     1021 cagcgtcagg aggatgccag aacgttccgg gatgtggccg gggaatatct ggaaatggcg
     1081 ggggccatag ccctggcggt taacccggtg acagggcctg tgctgatgtc ccgggctctg
     1141 cacgaccagc tcagtacgga agaagggcgt tctgaaatgg tggatggcgt gacacatttt
     1201 gtgtcggaac tatggcagcc cactgtggcc agtgcggccc cgggatccac cccgacccct
     1261 gacgataaaa tcgactgcca taaacatcct tcctcactga cgcagtttct cgcacaaaaa
     1321 aaagccgcct ttctggatgc ccccctggga acggccctta acctgctgaa tccgggcggt
     1381 atgctggaaa ccgcctttca gggagtgaat gcggttattg gcagcatcag taatctgttc
     1441 aaaggcgatg acgagccgcc cgcagcggaa tatatcgcgg aaggcgcgcg ggatgtgcgt
     1501 atcaacagcc agccggcgac acgcagcggg gcacgctgta cctgcgaggc cagggtggtg
     1561 aatgagccgg ggaacggggc gtttgtgtca ccggatgtgc gcattggcgg tccgttactg
     1621 gtggtcaggg atatcagaag cggcaggtcg cagataaccc tggtggcaac cgtggcgctg
     1681 atgtttcttc gcccgggcaa aatgctttct aaaatcgcct gttttgctgc cagtgtcggg
     1741 atgagcatgc tgacgcagaa ggccaccagt gcccttccgc atccggtcaa tgccgccacc
     1801 ggggccaaat acctggccga tgacgatgac ttcgatttca gtctgccggg ccactttccg
     1861 ctggactggc agcgcgtgta cagcagtcgt gacgaacgta cggaaggaat gtttggtcag
     1921 ggctggagtg taatgtatga agtcagcctc gtgtgcacac cgggtagcgc agacgaaaac
     1981 tgcatgacct ttgtgtcggg catggggcga cgcttggata tggaagccgt actgccgggg
     2041 ggcgggtttt acagccccgg tgaagggctg gcggtaaggc gtggtgagca ggggcactgg
     2101 cttatcagca gcgatgacgg gcagtgtttt ctgtttgagg ctgacccgca ccacccgcag
     2161 cgccagcggc tgaaaatgct gggcgaccgt aacagcaact gtctgaatct gtactacgac
     2221 gaccggggcc gcattgtgga aatcagcggc gaacagcagc gtccgtgcat ccgtctgtat
     2281 tacgaactgg cggcgcatcc ccggcgggtg acacagattt atcagcattt ccctgagact
     2341 gcacctttac tgctgcgtcg ttacagctat gacgaggccg gccacctgaa cggcgtgtac
     2401 gacagcacgg ggcacctgct gcgtgagttt gcttacgacg aaaaccactg catgacgctg
     2461 caccgccagc cgggcggcga agggtattat taccagtggg gctggtatga aggtccggat
     2521 gacgcggcat ggcgggtgac ggggcaccac acggacagcg gtgaacagta ccggctggcg
     2581 tgggacctgg cgcagcgcag gctgtgcgtg acggacggac tggggcgcac acgttatcac
     2641 cagtgggatg cgcagaatca ggtgacggcg taccaggacg aagccggaca ggtgacgaca
     2701 ttccgctgga gcgacgaaga acgactgttg ctggggatga ccgaccctca gggcggcaaa
     2761 tggcgctatg tgtatgacct gcaggggcat atcacggaga cgcacgaccc gctggggcgg
     2821 gtggagcagg cgcagtggca cccggtgtgg caccagccgg aaacggaggt ggacgcggcg
     2881 ggaaacctgc tggggaggcg cgcgggggaa agggccacgg cagaaaacag cgtggtgccg
     2941 ttcaaccggc tgatgtctta ccgtggtgtg cactaccgtt atgacgaatt tggccgtgcg
     3001 gtggaaaagg agggccggag cggaacgcag agttaccgtt atgatgcgga acaccggatg
     3061 gtggaagtga cgacagcgcg ggggacatac cggtatgtgt acgatgcact ggggaggcgg
     3121 acggaaaaac agcacatcag ccctgacggt aagccgtaca accggacaaa atttttatgg
     3181 gacggaatgc ggctggcgca ggagagcagg ccggagggaa caggcagcct gtacatctac
     3241 agggaccagg ggagttatga accgctggcg cgggtggata aggcgggcaa agagggcccg
     3301 aaccggatac tgtattttca cacggatgtg aacggcgcgc cggaagagat gacggacagc
     3361 gacgggaaaa tcgtgtggga gacggggtat caggtctggg gtaacacgat acaggagaag
     3421 gaccacggcg gggtggagca gaacctgcgc taccagggac agtacctcga cagggaaacg
     3481 gggctgcact acaatctgca caggtactat gatccggatg tcgggcggtt catggtaact
     3541 gacccgatag ggttgagagg cggtttaaat ctctacagtt atgcacctaa tcctctaaaa
     3601 tatgctgatc cacttggatt aactccatgt gctgttagta accaaaaagc gaatcgttta
     3661 ttagatagta gtgaaaccaa agttactgtt aggtctagaa gtgacgctga acaattgttt
     3721 atggatagat atttaggtca taattataaa aatatgacag gtgagtcagg ccccagcact
     3781 aagaacttaa tggaatatct tactgaaaat aaaacaaaag caggttcata tcattgggat
     3841 gatataaaag atccttctgt aacaaaacct tcatatagag tgtcaggtca tgggccagga
     3901 aatcctgatg gtgatttacc acatttacaa gttcatcaac atggtggaag tgttagacat
     3961 atatttttcc cgtgggagac ataataatga acaatctcaa tacattatta agtggaaagg
     4021 ttttgctcaa agaaattagt ccattaatgc ctggatatag aacatggatt gaaatttcat
     4081 tgatcgatga attaaaacca tcttatcctt ttagattaga tgaatatgct atgatagggc
     4141 attcacctta tgccaatgaa tgtaataaag atgaagctaa atttaaacta cgaatatcat
     4201 catttttagc ttctgacata gataatgaat acgatccatc atatgattat gttggaaaat
     4261 atgaaattat cgcttcactt aatgagttaa aaactaggtt aagtactctt catgtaaact
     4321 tggagcaatt tataaattca tcggaagatg atgagtatcc attataataa gacagaataa
     4381 aaagctagga aggttttaaa tcttcccagc ttttttaata tttaactttc gttcagcaca
     4441 ctaaacatcc catcccgcag ttcctgcacc tcgtcactgt cagcgatgat ggtcaggccc
     4501 ccctccgaaa tcttcaccgt gacaccccgt ccggtatcaa atccggcctc tttcagccag
     4561 tcgcccttaa ggtgcagact gggatgttgt gaataatacc gggctatacc ggttttgcgg
     4621 tcctggtggc gggctctgac atagccgact ttatagcggc gctgtgtttt ggaaatagtg
     4681 acttctggcg taatatcgtg ttcagccatg attaactacc ttgattagtt gcttgcgggg
     4741 agcgcgcttt cgtaatcttg ccctgctagc ggattaactt cacaaacatc atttaccaac
     4801 gcctacactt actcctgtat accgttcagg agcagtaatg aatctacaac gatttgatga
     4861 cagcacccta atccgtatct tcgcccttca tgagttacat cgactgaaag aacatggctt
     4921 aacgcgcggg gcgcttctcg attatcacag ccgctataaa ctcgtctttc tggcccattc
     4981 tcagcccgag taccgcaaac ttggcccgtt tgtggctgat attcatcagt ggcaaaatct
     5041 ggatgatttt tacaaccagt accgccaacg cgtaattgtt ttgctttctc atcccgccaa
     5101 cccgcgcgat cacaccaacg ttttgatgca cgttcagggt tattttcgcc cgcatattga
     5161 ttccacagaa cgccagcagc tggctgcgct tatcgacagt tatcgccgtg gcgagcaacc
     5221 acttcttgcg ccgctgatgc gtatcaaaca ctatatggcg ctttatcctg acgcctggct
     5281 ttcagggcag cgttatttcg aactttggcc gcgtgtgatt aacttgcgcc attcaggagt
     5341 tttatgacta cccatctggc ctggtttcgc caggatttac gtctgcacga taatctcgca
     5401 ctggctgccg cctgccgcaa ttcgtctgca cgcgtgctgg cgctgtatat cgctacacca
     5461 cgcaagtggg agacgcataa catgtcgccg cgtcaggctg aacttatcaa tgctcaactg
     5521 aatgggctac aaatcgcgct ggcggaaaaa ggtattcctt tattgttccg tgaagtggat
     5581 gactttgtcg ccagtgtcga aatagttaaa caggtgtgcg cggaaaacag cgttactcat
     5641 cttttttata actatcagta tgaggtgaat gagcgagcgc gggatgtgga cgttgaaaga
     5701 gcgctgcgta acgtggtgtg tgaaggattt gatgacagcg tgatcctgcc gccgggggcg
     5761 gtgatgaccg gcaatcacga gatgtacaaa gtctttacgc cttttaagaa tgcctggctg
     5821 aaacggctgc gggaagggat gccggagtgc gtcgctgcac caaaagttcg tagtagcgga
     5881 tcgataaagc ccgcgccatc cattacgctg aattatcctc gtcagtcttt cgatactgcg
     5941 catttcccgg tggaagaaaa agcggcgatt gcgcaattac gccagttttg cgagaacggt
     6001 gctggagagt atgagcaaca acgagatttt ccggcagtgg aaggcaccag ccgtttgtcc
     6061 gccagcttgg caacgggcgg gttatcgcct cgccagtgtt tgcatcgctt gttggcggaa
     6121 cagccgcagg cgctggacgg tggggccggt agtgtctggc ttagtgagct gatctggcgc
     6181 gagttctacc gtcatctgat gacgtattac ccctcgttgt gtaaacatcg tccatttatt
     6241 gcctggacgg atcgtgtgca gtggcaaagc aatcccgcac atttacaggc ctggcagaaa
     6301 ggcaaaacgg gatacccgat tattgatgcc gctatgcgtc agcttaacag cactggctgg
     6361 atgcataacc ggctacggat gattacagcc agttttctgg tgaaagattt gttgatcgac
     6421 tggcgcgaag gcgagcgata tttcatgtcg cagctgattg atggtgattt ggcagctaat
     6481 aacggtggct ggcagtgggc ggcttctacg ggtactgatg ctgcgccgta ttttcgtatt
     6541 ttcaatccga taactcaggg cgagaaattt gaccgtgagg gcgagtttat tcgtcgatgg
     6601 ttaccggagc tgcgcgatgt accagggaaa gcggtgcatg agccgtggaa gtgggcgcag
     6661 aaagcaggtg tgaagctgga ttatccgcaa ccgatagtcg agcacaaaga ggcgagagta
     6721 caaacgttgg cagcgtatga ggcggcgcgg aaggggaaat aacagacgcg tcaggcaatc
     6781 gagcccagat gccggatgcg gcgtgaacgc ctaatccagc ctacaaattt gtgcaaattc
     6841 aataaattgc aggaaatacg taggcctgat aagacgcgtc aagcgtcgca tcaggcatcg
     6901 gtgctggcct attatgactc cagcgccagc gcgcggtttc tgaatttcag cgcctgatac
     6961 agccaaatca tcagtaccag tcctacacac gccagtgcgc cccaggtaat ttgatcaaat
     7021 acttcaatat atgcattgat ggagtagttg atcgccccgg aagcatcaaa cgaagcctgc
     7081 gatgtctgat cggcaatcac gcccgccaga tagttcgcga tcgcgccaga aagcagcatg
     7141 tagatgccgg ttaatacgcc ggtcacgccc ggaatttcaa tgcgcgtaat ttgcgacatg
     7201 gcaaccgggt cgataaacag ttccgcaaag cccatcaccg cgaggcctaa caccatcagt
     7261 ggcagagaag agtgaccata cattgcggac cagcgggcgc ttaaggtcag aatgcagaat
     7321 ccggcgctca tcaggccaag gccaagagca aattttcccc agatgcgcac ggtacgatta
     7381 cctgcgacgc tttctttaac cacccacgcc aggaacacac cgcacagcat aactgcgaag
     7441 gcattaatcg actggaacat cgcggtcgga acggtataac caaacatatc gcggttaacg
     7501 aagcggtcga tataaaggct aatcgagcta ccgccctgtt gtgcgaaggc ccagaacaac
     7561 atactgaaga aggtgagcgt cacaatcagc cccagctcct tccgctgttt ctggttttct
     7621 gctttgcgat aaatttttgc cagtacaccc aggccaataa tagtcgcgac aattaaggcg
     7681 tataccgacc actctttcca gaacagcacg gtaatcagtg caggcgttgc caccagcaga
     7741 accagcagcc atccccagtt cggcaggaga aagtttgtcg cacgcagtac ttttttgtta
     7801 acgccgcggg tatgagtgaa atgacgattg ccacataaga aaatgaccag acccgcgatc
     7861 attccaaccg ccgccaggcc aaagcccatc gcccagctgt actcttcctg ggcgtaacca
     7921 caggcgatag gtgcgataat agaccccacg ttacccgccg catacatcag cgagaaaccg
     7981 ccatcacgac gcggatcggt tggctcatac agctcgccga gcagacagct tacgttagat
     8041 ttaaacaggc catagccgca gacgataatc gccagggaca gatagaggaa tgacggatgg
     8101 atctcactgg cacccagcac cacatgaccg atcgccatca acaacgcccc cagcatcacc
     8161 gccatgcgat tgccgagaac tttatccgcc aaaaagccac cgaggattgg cgtgacatac
     8221 accagcgaac agtaggcgct aaataactcg taggcgtgag tatcgttgta ttttagttga
     8281 ttggtgagat agagaatcag cagggcacgc atgccgtaaa agctaaaata ttcccagatt
     8341 tgcagcgcaa cgacatagta aatagcgcgc ggttgtgatg cgtgtttatt cataataatt
     8401 ccacaattga cggcaacgtc acgtaatagt tgcgcggcgt gcttgtgttt ccttaaagtt
     8461 aaaactttga tacagatctg attatttatg caatatgctg tctgattgca taaatataca
     8521 ttagctgaag cgtgatgata taaacaattt gcgtcttttg gcgtcgggtt tgtttcttaa
     8581 tatgtcaaaa gattggctat cgacgaacag gcgatgtaac gctatgttaa cggtgtagct
     8641 atcagaatac ggaaggcgcg aagatgaaaa acaccgaact ggaacaactg attaacgaaa
     8701 aactgaacag cgcggcgatt agtgattacg cgccgaatgg tttgcaggtg gaaggcaaag
     8761 agacggtgca aaaaattgtt accggtgtca ccgccagcca ggctttgctc gatgaggcag
     8821 tgcgtctggg cgcggatgcg gtcattgtgc atcatggcta cttctggaaa ggagagtctc
     8881 cggtcattcg cggaatgaag cgtaaccgtt taaaaacgtt gctggcgaat gatatcaacc
     8941 tgtatggctg gcatttgccg cttgacgcac atcctgagct gggcaataac gcacagctgg
     9001 cggcgttact ggggatcacg gtcatgggcg aaattgagcc gttggtgccg tggggagagc
     9061 tgaccatgcc tgtgccggga atggaactag cctcctggat tgaagcgcgt ctgggacgta
     9121 agccgttatg gtgtggcgat accggacctg aggtggtcca gcgcgtcgcc tggtgcacgg
     9181 gcggcgggca aagttttatc gatagcgccg cgcgttttgg cgtggatgct tttattactg
     9241 gcgaagtttc tgaacagacc attcattcag cccgcgagca gggattgcat ttttatgctg
     9301 caggtcacca tgccactgaa cgcggtggta ttcgcgcatt gagcgagtgg ctgaatgaaa
     9361 ataccgatct tgatgtgacc tttattgata ttcctaatcc tgcataacga ataatcagag
     9421 ggatcgaaag tgcaacgagc gcgttgttat ctgataggtg aaacggcggt agtgctggaa
     9481 ctggaaccgc cggtgacgct ggctagccag aaacggatct ggcgactggc gcagcgtctg
     9541 gtggatatgc cgaatgtggt tgaagccatt cccggcatga acaatatcac ggtgattttg
     9601 cgtaatcctg agtcgctggc gctggatgcc atagagcgtt tgcaacgctg gtgggaggag
     9661 agcgaggccc tggaacctga gtctcgcttt attgaaattc cggtggttta cggtggtgca
     9721 ggcggaccgg atttggcggt ggtcgcggcg tattgcgggt tgagcgaaaa acaggttgtt
     9781 gaattgcact cctccgtgga atacgtggtc tggtttttag gttttcaacc gggcttcccg
     9841 tatctcggga gtttgccgga acaactacac acgccacggc gcgctgaacc gcgcttgctc
     9901 gttccggcag gttctgtcgg gatcggcggg ccgcagactg gtgtttatcc gctggcaacg
     9961 ccgggtggct ggcagttgat tggtcatacc tcactcagcc tgtttgatcc ggcgcgtgac
    10021 gaacccatct tattacgtcc gggagacagc gtgcgatttg taccgcagaa ggagggaata
    10081 tgctgaagat tattcgtgcg ggcatgtaca ccactgtgca ggatggcggt cgtcacggtt
    10141 tttgccagtc gggtatcagc cactgtggcg cactggatat gcctgcgctg cgcattgcta
    10201 acctgctggt gggtaatgac gccaatgccc ccgcgctgga gatcacgctc ggtcagttaa
    10261 cagttgagtt cgaaactgat gggtggtttg ccctgacggg tgcaggttgc gaagcgcggt
    10321 tggatgataa cgccgtctgg accggctggc gattgccgat gaaagcaggc cagcgtttaa
    10381 cgcttaaacg cccgcagcac gggatgcgca gttatctggc ggtcgcgggt ggtattgatg
    10441 ttccgccggt aatggggtca tgcagcaccg atctcaaagt ggggattggc gggctggaag
    10501 gccgtttact gaaggatggt gactgactcc cgattggcaa atcgaagcgt gattttatgg
    10561 aagcgcaggg cgttaaacag ctgctgtggg gcaaccgcat tcgcgccttg ccggggccgg
    10621 aatatcatga gttcgatcgc gcctcgcagg atgcattctg gcgttcgccc tggcagctta
    10681 gctcgcaaag taaccgaatg ggctatcgtt tacaggggca aattttaaaa cgcatcaccg
    10741 atcgcgaact gttatctcac ggtttgttac cgggcgtggt gcaggtgccg cataacgggc
    10801 agccgattgt gttgatgaac gacgcacaga ccaccggtgg ttatccacgt attgcctgta
    10861 tcattgaggc tgatatgtac catctggcgc aaattccgct cggtcagccg attcattttg
    10921 tccagtgttc actggaagag gcactgaaag cgcggcaaga tcagcaacgt tattttgaac
    10981 aattagcgtg gcggctgcac aatgaaaatt gacctgaacg ccgatctggg cgaaggctgc
    11041 gccagcgacg cagagctatt aacgctggtt tcctctgcca atattgcttg tggatttcat
    11101 gcaggcgatg cgcaaaccat gcaggcttgc gtgcgtgaag caataaaaaa tggtgtcgcg
    11161 attggcgctc acccgagttt tcccgacagg gaaaattttg gtcgcagcgc catgcagctg
    11221 ccgccggaaa ccgtttacgc ccagacgctg tatcaaattg gcgcgctggc aacgattgcc
    11281 cgtgcgcaag gcggcgtaat gcgtcatgtc aaaccgcacg gcatgttgta caaccaggcg
    11341 gcgaaagaag cacaactggc agacgccatc gccagagcgg tatacgcttg cgatccggca
    11401 ttggttctcg ttgggctggc aggaagcgag ctgattcgtg caggcaagca atatggcctg
    11461 acaacgcgcg aggaagtgtt tgccgatcgc ggttatcagg ctgacggctc gctggtgccg
    11521 cgaagtcagc caggcgcgtt gattgaaaac gaagaacagg cgttggcgca aacgctggag
    11581 atggtgcaac acggcagagt caaaagtatc accggcgaat gggcaacggt cgcggcgcaa
    11641 acggtctgcc tgcatggcga cggcgagcac gctctggcgt tcgcccgccg attacgagct
    11701 acatttgcca aaaaggggat tgttgtcgca gcataacccc gcttaataaa gaatgaaaaa
    11761 aggatatcac catgcctgaa ggcccggaga tccgccgtgc agcggataac ctggaggcgg
    11821 cgatcaaagg caaaccacta actgatgtct ggtttgcctt cccgcagtta aaaacttatc
    11881 aatcacaact tatcggtcaa cacgttaccc atgtggaaac gcgtggtaag gcgttgttaa
    11941 ctcattttcc caacggttta acgctctaca gccataatca gctttacggc gtctggcgcg
    12001 tggttgatac cggcgaagag ccgcagacca cgcgagtatt gcgggtaaaa ctgcaaacgg
    12061 ctgacaaaac cattctgctt tatagcgcct cggatattga gatgttgagg ccggaacaac
    12121 tgaccacgca tccgttttta caacgcgttg gtcccgatgt gctggatccg aatctgacgc
    12181 cggaggtggt aaaggaacgg ttattgtcgc cgcgctttcg taaccgtcag tttgcaggat
    12241 tactgctcga tcaggcgttt ctggctgggc ttggcaatta tttgcgggtg gagatcctct
    12301 ggcaggttgg gttgactgga aatcataaag cgaaagatct caatgcggcg caactggatg
    12361 cactcgcaca cgcgttactg gagattcctc ggttttctta cgctacgcgg gggcaggtgg
    12421 atgagaataa gcatcatggg gcgctgtttc gctttaaggt ttttcatcgt gatggcgaac
    12481 tgtgcgaacg ctgtggcggc atcattgaga aaaccacgtt gtcctcgcgc ccgttttact
    12541 ggtgtccagg ctgccagcac taggccgacc gcttcggcgc ataggttgaa ataaaccgcg
    12601 caatggctgg ccccgtcagc aaaatactga acaggcgcag ggtttgcatc gccataataa
    12661 gcgccatatc ggcattgctc cctgcggcga tgaccgccac ggtatcaagc ccgccgggac
    12721 tggtggcgag gtaggcagtc ataaaatcaa tatgcataaa ccgggtcagt ccccacgcca
    12781 tacctgcaca aatagccagc agagcaaaaa tcgacagcag gatttgcggt agcgggcgta
    12841 gtgcccgcag taagatttgt ttatcgaaac caagaccaat ccgccagcca attgccatat
    12901 acgccatcgc cagcagccat tccggtagtt cgatggtgat gagctgacca gactggagca
    12961 ctgcgcccgc cagcatcggg atgagcatcg tgccggaagg aagacgcagc atgcatcccg
    13021 ccgtaccggc aacgactgcg agcaaaatcg taagcaggag attaatgctc actggcggga
    13081 accagacgat ttgctggtta accgcttcag cgttatcacc cagcatcatg cgggtgacca
    13141 gaacagcggc ccccgcgacg aacagcactc gcagatattg cataaacgcc accaggcgaa
    13201 tatctgcgcc gtaatcttgc gccatagcga ccattgccgc tgcgccgcct ggagaagaac
    13261 cccaggcacc ggtatttcca ggcagtgagc tatagcgcac caataaccaa cccacgatgg
    13321 cgctggaaag cagcgtcacc agtaaaatag caagcacgat cggccagttg gcggccagcg
    13381 tggtgagaat cgaaccagtg aggttttgcg caatcatgca gccaagaatg gcctgcgcgg
    13441 cgagaaaagc ggagcgggga agttgcagag taattccgcg catactgaag atgatcccgg
    13501 cgatcatcgg cccaagtaat agcgccgccg ggagatgtac cgcgaggaaa ccaatagaaa
    13561 ggagaagtga tagcacacat aacattcccc actgcaaaac tggcatcctt cgctccctca
    13621 ttattttctc tttggtgtat cgatacagca taaagaaagc cgggctagga aaaaataaca
    13681 gcaatcaagg aaaaggggaa aatcagcaat tttctgaaag agatgccctt tccggctgca
    13741 aagggcatca tggcaaatca gttatagtta attttaaaaa gcaccactgt atcgaagggc
    13801 ccagcctcga tgggggtacc tgcaatggac gaaagttcag ctgtataggt tttttcgtag
    13861 accaacatgt cgctggtgaa atcggcgtat tcagcatatt tattgaaggt ataaggcgag
    13921 gcgttttcat ccagcaaacg tagcttcaga ccattaccaa tcaacaatgc tttatcttct
    13981 tcaaccaacg tttcttcggt ataaaaggag gaactgactt taaatccgtc ggtgcattgg
    14041 tcattttgtg attttgtcgt cttaatactg aatgttttcg acattgtgtg gcgacgaata
    14101 tccagcaaat taaatttacc aaaatcaatc atctgtgttt ccggtgaaat ggaaaaattg
    14161 acgctgcaat ccagcacgcg aatgttttcc agaccagtaa tatgatattt caggttttgg
    14221 gcagtagggt cttcgttaac gctaccggca ccgtcaaatt gcacgacgat atagtcgcta
    14281 agcgtacttt gataatcatg cggcggcatt tctcgtattt tgacgtataa ccgcatgcga
    14341 gcgagaaagg ttcgcgacat gtgaatatta gcgggatcac ccgagcaaat tttgttctgc
    14401 catcccatca ggggtataaa tgtcaatgtt cttactgtcg atacactggt tagtgtcgat
    14461 gcggcttttt ccctggttgg cgtcataatc aacgccattg taagttactc cgagttgata
    14521 ataacgatcc tgcacccccg ggtaggggtt tacccatacg tagacatgtt cgctttcaaa
    14581 gttgccgttg gtattgttgt cacaatagac gggaatttta atatcgtcag attcccagat
    14641 cttattttaa tatcgtcaga ttcccagatc ttatcgccga gtttggcatt gcctggtaca
    14701 gcaaaaggtt gaatcgcttc tgatttttct accgagccac cggatgaacc aaagtagcag
    14761 ttcagggcaa gcgcagactt caacgacaga aataacagac agatgacgag caacactccc
    14821 ttgccagcac tcatttcttc gcctccacgg cttgtaaatg gcattgtccg ctgcagttga
    14881 gcgtcgtcat gcgtaacgcg ccgtaatcat ccatataacc gagataaaac tggctgccgc
    14941 tataatttcc ggtgtttgtg ttgaccgtac taaaaggcgc aaccatcacg gttttgaagc
    15001 cggggagaac gcctttttca tttcgtccca ggtaggcaat ggtcagataa tacgcggtag
    15061 gatttttcag tgtcagttgg ttgccctgct ggctgacctg taactgcagt tcgacctttt
    15121 ctcctgcttt cttgcgtaat gcagccggtc gccagaacac cttgatacga ctctgaatgg
    15181 cgacttgcag aacggcatgg tcgctgtttt tttccggggc gggaggaatt tcacgcatat
    15241 tgtagaaaaa tagcgtttcg cgatcgcccg gtaattttgt ggtcgaggct tgctttacta
    15301 tccgtacctg tgttgttgct ttcggttcca gacgttgtat cggcggcagg gcgaccagaa
    15361 gatcatcgct tttctcccct ttttcatttt cgagccagga ataagcgagg taggggagtt
    15421 tatcgctacg attatccacc cgcagactgg tagctttatc attagcgtta aacacaatac
    15481 gagtgcgatc gggttgtagt gcagcgtggc tgcacagact ggcaaccaac aacaaaaggg
    15541 gtagtccttt cataaatgtc attttcattt ccttaattaa tgacaaggta aaatcaggcg
    15601 atttgcggtg tcttccatat gttcaggaag atggagcgag caatgctggc tatcacccca
    15661 gaccacggta aacttttgat tttcagcgac tccgcttaac caggcatgtc cttcctcgcc
    15721 aaccatcccc acgctaatgc cgctgtcatc ctggcggata tccgctccga ggggaggaaa
    15781 ctgaccgctg gcgttgcgaa tgatgacgtt accgtcttta ccggaacggg aagccagtga
    15841 tttgtaacct atcgcgcctt caatccacgt ttctttgata acgttttctg caactgttac
    15901 gccgtcgggt aagtcattca tgttcaccgc cacggtggaa ggctggtaac tggaaatcaa
    15961 cggcaccacc gcaatgccaa aatggttggt gtagtcgaga ttgccctgaa ccggaatatc
    16021 tgccacgcca tcggtgctga ccatcaggcg tggttcattg gtggagctgc ggcgatgaaa
    16081 cgctgcacca tattgggttg cggtgaaaga accgctccag ctgctgctga cggaactgta
    16141 atcattggcg gcataggtac cagaaatatc ccactcaccc gctgaactca ggtgctgata
    16201 gttaccgctc acctgggctc cattgtcagg acggtcggat tgcagtccgg cagacatgcc
    16261 ccagctatta cgttcatcca gcgtatcgtt ccacgacatg cggtgtgtgg tgctgtggct
    16321 actgttttgc atgtcatagc caacccgacc accgttaccg aaggggagcg aaatcgacag
    16381 gtaaatctgg ttgtcgcgat ctttatcttc gtaatgggtt gtattgaatg acgtagaaat
    16441 agagatatct ctccagtcac caatatcaac attaaaacct gccgtgatgt tggcggtcgt
    16501 ggaggcgtct gcattccacc aggtttgatg tagcaggttg gcgtaaagat tgaggtttag
    16561 tggggtaatc ggttggccca cagataagct gatcgtctgt ttttcgtcct gcgcatcgct
    16621 gtcgttgtat ttgtgatcca gatagttggc gtagctgtga aaatgacgat cggagaaacg
    16681 ataagcggcg agggaaatcg tgctgttagt ggcatccact tgtttgctgt aattaaaacg
    16741 gtagcttaag ccccgctcgt cctgctgagt atcaaaatgg ctactggccc acgtgacatc
    16801 aaacgacagc gcaccaagcc acagcatatt ttgcccaata cccattgcgg cagaatggta
    16861 gtcatcacca gaaagcagca ggccgctgta cagcgaggtg tttgacagca tcccccagga
    16921 aacttcattg ctaaaaaagg tttcattttc agtttgatgt gacatggagg gccgtggctg
    16981 acccgcggcc agtttatagc gaacctgtcc ctgacgagtc aggaagggcg tcgatgctgc
    17041 tgaaacctgg aaattgttca cccgaccatc ttcttccgtc actttgacat ccagtgtgcc
    17101 ctgaacagac tgattaaggt cgtcaatgat aaatgggcct ggtgggattt ttttctggta
    17161 aatgacgcgg cctgattgac tgatcgtcac cgtggcattg gtctgtgcaa taccgctaat
    17221 ttgtggggcg tagccgcgta gttcccatgg caacattcga tcgtcacttg ccagtgccgc
    17281 gccggtataa gaaaaaccgt cgaaaatatt gggactaaaa tccgtttcgc cgagggttaa
    17341 ttttgaacct aattgcggta acgggcgaaa aagataggta cgtgatattt cgcctgactg
    17401 ttcatggtta tcatcgctat cagtatgatt caactgataa tcactgcgta agcgccatgc
    17461 ccccgtgttg attccggctg ttccataggc gttaagatta gtgctgctgc tgccatcctg
    17521 tgggcggtag ctgctggcaa acaagttgta atccatcagg acaccggcga caccttcttt
    17581 ccatgtggag ggtggggtcc agttttctga gtgccacgcc agccaggctt gcggaatact
    17641 aatattcagt tgctgattgg cttgatcgaa attgaagagc atttcaggtc gggagctaaa
    17701 atcgacgcac tgatttatca atggtaacga ctgacggact tcaggtttta agccaaattt
    17761 atcgaccagt aaatcattga tgcacggaat ggttttatcg tcatttttgt gccagttaat
    17821 cttttgctcg ttactgattt ggttattatt taccgcaacg ctaacaaaat actcaccggg
    17881 agcaatgact cctttttcct ttaataatga aatatcaatg cggtcgcgca ttgatttatc
    17941 gagaacattc agattgaatt cgaccgccaa tgcgcaaggc atcgccacga ccaggcagga
    18001 tacaaaagag agtcgataaa tattcacggt gtccatacct gataaatatt ttatgaaagg
    18061 cggcgatagc gccgccaaag taatacttat ttataatcca gcacgtaggt tgcattcgcc
    18121 gtaactgcgc ctggcgttac aggtgtagcc tgatcaattt gttccatcca ggcaaagaaa
    18181 ttcagcgttt gttcgctgga ggtcggagcc aggtcgatat ctggagtagc ggtgcctaaa
    18241 acgatattgc tttgatcttt attcattaaa cgtacgccga cgccggtggc ttcacccgtg
    18301 ctggtgttgt tgagaatcgg atctgcgccg gtggttttcg ctgagctatc aaaggtgact
    18361 gcaactttag aaatcttgcc accggaaccg ttgctggagt tttccagatc acagttgact
    18421 aagagcaggt cgacagcaac ggcatcgctg tgatgtgact gattaatgtg gctggtggtc
    18481 acctgaccga gttcaacctc tttatcaata tcatcctgat gtatttcaca cggtgcttca
    18541 ataacttcac ctttaaagtg aatttcaccg gatccttcat cagccgcata aacaggtgct
    18601 gtgaacagca gagatacggc cagtgcggcc aatgtttttt gtcctttaaa cataactgag
    18661 tcctttaaca gaaaagaa
//
LOCUS       DAZPCS010000069        18578 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZPCS010000069 DAZPCS010000000
VERSION     DAZPCS010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18578
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.069"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            91..540
                     /gene="alaE"
                     /locus_tag="ACHUQ7_002287"
     CDS             91..540
                     /gene="alaE"
                     /locus_tag="ACHUQ7_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFR1135677.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(577..921)
                     /locus_tag="ACHUQ7_002288"
     CDS             complement(577..921)
                     /locus_tag="ACHUQ7_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFR1135678.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            1073..1402
                     /locus_tag="ACHUQ7_002289"
                     /pseudo
     CDS             1073..1402
                     /locus_tag="ACHUQ7_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
     gene            1650..1895
                     /gene="nrdH"
                     /locus_tag="ACHUQ7_002290"
     CDS             1650..1895
                     /gene="nrdH"
                     /locus_tag="ACHUQ7_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFR1135679.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            1892..2302
                     /gene="nrdI"
                     /locus_tag="ACHUQ7_002291"
     CDS             1892..2302
                     /gene="nrdI"
                     /locus_tag="ACHUQ7_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFR1135680.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            2275..4419
                     /gene="nrdE"
                     /locus_tag="ACHUQ7_002292"
     CDS             2275..4419
                     /gene="nrdE"
                     /locus_tag="ACHUQ7_002292"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFR1135681.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSCITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            4429..5388
                     /gene="nrdF"
                     /locus_tag="ACHUQ7_002293"
     CDS             4429..5388
                     /gene="nrdF"
                     /locus_tag="ACHUQ7_002293"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFR1135682.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            5743..6945
                     /gene="proV"
                     /locus_tag="ACHUQ7_002294"
     CDS             5743..6945
                     /gene="proV"
                     /locus_tag="ACHUQ7_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFR1135683.1"
                     /translation="MAIKLKIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            6938..8002
                     /gene="proW"
                     /locus_tag="ACHUQ7_002295"
     CDS             6938..8002
                     /gene="proW"
                     /locus_tag="ACHUQ7_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFR1135684.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALITWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            8060..9052
                     /gene="proX"
                     /locus_tag="ACHUQ7_002296"
     CDS             8060..9052
                     /gene="proX"
                     /locus_tag="ACHUQ7_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFR1135685.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            9244..10423
                     /locus_tag="ACHUQ7_002297"
                     /pseudo
     CDS             9244..10423
                     /locus_tag="ACHUQ7_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            10547..11284
                     /gene="ygaZ"
                     /locus_tag="ACHUQ7_002298"
     CDS             10547..11284
                     /gene="ygaZ"
                     /locus_tag="ACHUQ7_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFR1135686.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALSVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            11274..11609
                     /gene="ygaH"
                     /locus_tag="ACHUQ7_002299"
     CDS             11274..11609
                     /gene="ygaH"
                     /locus_tag="ACHUQ7_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFR1135687.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            11700..12230
                     /gene="emrR"
                     /locus_tag="ACHUQ7_002300"
     CDS             11700..12230
                     /gene="emrR"
                     /locus_tag="ACHUQ7_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFR1135688.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRKSDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            12357..13529
                     /gene="emrA"
                     /locus_tag="ACHUQ7_002301"
                     /pseudo
     CDS             12357..13529
                     /gene="emrA"
                     /locus_tag="ACHUQ7_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            13546..15084
                     /gene="emrB"
                     /locus_tag="ACHUQ7_002302"
     CDS             13546..15084
                     /gene="emrB"
                     /locus_tag="ACHUQ7_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFR1135689.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLNSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFCASAWPQFIQGFAVVCFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(15148..15663)
                     /gene="luxS"
                     /locus_tag="ACHUQ7_002303"
     CDS             complement(15148..15663)
                     /gene="luxS"
                     /locus_tag="ACHUQ7_002303"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFR1135690.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILEREVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(15813..17369)
                     /gene="gshA"
                     /locus_tag="ACHUQ7_002304"
     CDS             complement(15813..17369)
                     /gene="gshA"
                     /locus_tag="ACHUQ7_002304"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFR1135691.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(17442..17870)
                     /locus_tag="ACHUQ7_002305"
     CDS             complement(17442..17870)
                     /locus_tag="ACHUQ7_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFR1135692.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(17867..18433)
                     /gene="yqaB"
                     /locus_tag="ACHUQ7_002306"
     CDS             complement(17867..18433)
                     /gene="yqaB"
                     /locus_tag="ACHUQ7_002306"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFR1135693.1"
                     /translation="MYERYAGLIFDMDGTILDTAPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         4402 a         4812 c         4713 g         4651 t
ORIGIN      
        1 tatacgctat gcaaaaatgc agatggcaat gagatccact gctttcatct ccattaacat
       61 cccattacgc ttttattaag gagcattagc atgttctcac cgcagtcacg cttgcgtcat
      121 gcagttgcag atacgttcgc gatggttgtt tactgttctg tcgtgaacat gtgtattgaa
      181 gttttcctct ccggaatgag cttcgaacag tctttttatt ccagattggt agcgattccg
      241 gtgaacatct taattgcatg gccatacggt atgtaccgcg atctgtttat gcgcgcggca
      301 cgcaaagtta gcccgtcggg ctggataaaa aatctggctg atatcctggc ttatgtgacg
      361 ttccagtcac cggtgtatgt ggcgatcttg ttagtggtgg gcgcagactg gcatcagatt
      421 atggcggcgg tcagttcaaa catcgttgtt tcgatgttga tgggggcggt ttatggctac
      481 ttcctcgatt attgccgccg actgtttaaa gtcagccgtt accagcaggt aaaagcctga
      541 ctcttctttt cgcgactggc atcgccagtc gcgtctttaa ctggcttcgc caaataaacc
      601 attcaaataa cgttcaagcg caacacgcga actaaagcca tgcgggatac cgtaatgctc
      661 gtgtcgctcg cctgctaaat agagcggaaa ttgctggtaa tgatcgcaag ttttaatatc
      721 ggaagcgggt tcagccagtg ttgaactgcg ttcttttaat gttggatgaa ttaccagagc
      781 ggtacgtccc attcgcgctt cgcgattaac atagacataa ttttccccac ggcggtaacc
      841 atacgctttc tgggttacca catcgacagt aaaaccgact ttttcaagta cgcgcgccac
      901 ctcgtctggt cgtaaataca tatttgatcc tcgttatttt caaccgcggg cctaccttac
      961 ctgattgcgc attcacaacg ctttcagaaa agtccataaa gccgcgatga acctcagtta
     1021 agaaaattat ggtctacact gaaaattaca tcgaattcta atgggggatc atatgtttaa
     1081 ccgaccgaac cgcaacgacg ttgatgatgg cgtgcaggat atccagaatg atgtcaatca
     1141 attagctgac agcctcgaat ctgtattgaa gtcctggggc agcgacgcca aaggggaagc
     1201 tgaagccgca cgcagtaaag ctcaggcgtt gctgaaagaa accagggcgc gaatgcatgg
     1261 tcgtactcgc gtccagcaag ccgcgcgcga tgccgttggc tgagcggatt cttttgttcg
     1321 tgaaagaccc tggtgtagcg tgggtacagc agctgcggta ggtattttta tcggcgcact
     1381 gttaagcatg cgcaaatcgt agtgcaaaaa tgataataaa tacgcgtctt tgaccccgaa
     1441 gcctgtcttc ggggtttctt tttgcctggt gaatcacaaa aatccccctc ccccgtcacg
     1501 ctcatatcca gggtaatttc gatcactatt tgctatatat tgtgtagttg aatctttttt
     1561 caactacatc tagtatctct gtatcaacag agagacaacc cgacgcgtat catcgcgccg
     1621 tatcttcatt ttaaacggaa atacgaatca tgcgcataac tatttatact cgtaacgatt
     1681 gcgttcagtg ccacgccacc aaacgggcga tggaaaaccg gggctttgat tttgaaatga
     1741 ttaatgtcga tcgcgttcct gaagcagcgg aagcgttgcg tgctcagggc tttcgtcagt
     1801 tgccggtagt gattgctggc gatcttagct ggtctggttt ccgtccggac atgattaacc
     1861 gtctgcatcc agcgccacac gcggccagtg catgagccag ctcgtctact tctccagcag
     1921 ctccgaaaac acgcagcgtt ttatcgaacg tttaggtctg cccgcggtgc gcatcccgct
     1981 caatgagcgg gaacggattc aggtagacga gccttacatc ctgatcgtgc cctcttacgg
     2041 cggcggcggt acggctggcg cggtgccacg acaggtaatt cgctttttaa acgacgagca
     2101 caaccgggcg ttgcttcgcg gcgttattgc ttctggtaat cgcaactttg gtgaggcgta
     2161 tggccgcgcc ggagatgtga ttgcccggaa atgcggcgtg ccgtggctgt accgttttga
     2221 actcatgggt acgcaaagcg atatcgaaaa cgttcgtaaa ggagtaaccg aattttggca
     2281 acgacaaccg cagaatgcct gacgcaggaa acgatggatt accacgcgct gaatgcgatg
     2341 cttaatcttt acgatagcgc aggtcgcatt cagttcgata aagaccgcca ggccgttgac
     2401 gcctttattg cgacgcatgt gcgtccgaac agtgtgacct tcagtagcca gcagcagcgc
     2461 ctgaactggc tggtcaacga aggttactat gatgaaagcg ttcttaatcg ctactctcgc
     2521 gactttgtca ttacgctgtt tgcccacgca cacaccagcg gttttcgttt ccagacattc
     2581 ctcggggcat ggaagtttta caccagctat acgttgaaga cattcgacgg taaacgttat
     2641 ctggaagatt ttgccgatcg agtcacgatg gtggcgctga cgctggcaca aggcgatgag
     2701 acgctggcgt tgcaactgac cgatgaaatg ctgtcaggac gctttcagcc agccacgcca
     2761 acattcctca actgcggtaa gcagcagcgc ggcgaactgg tttcctgttt tttgctgcgt
     2821 attgaagaca atatggagtc gattggtcgg gcggtaaatt ccgcactgca gctgtcgaaa
     2881 cgcggcggcg gcgtagcatt tttgctgtcg aatctgcggg aagcgggcgc gccaattaaa
     2941 cgtattgaaa atcaatcttc tggcgtaatt ccggtgatga aaatgttgga agacgcattt
     3001 tcctatgcca accaactcgg cgcgcgtcag ggggcaggtg cagtctattt acatgctcat
     3061 catcccgata ttctgcgttt tctcgacacg aaacgggaaa atgccgacga aaaaatccgc
     3121 attaaaacac tgtcgctggg cgtggtgatc ccggatatca ctttccatct ggcaaaagag
     3181 aatgcgcaga tggcgctgtt ttcgccctat gacgttgagc gagtttatgg caagccgttt
     3241 gccgatatcg ccattagcga acactatgac gaactggttg ccgatgaacg catccgcaaa
     3301 aaatacctca acgcccgtga tttcttccag cgactggcag aaatccagtt tgagtccggc
     3361 tatccctaca tcatgtatga agacacagta aaccgtgcta accctatcgc cgggcgcata
     3421 aatatgagta atctctgctc agaaattttg caggttaaca gcgcctcaga gtatgacgag
     3481 aatctcgact atgcccgcac aggccatgat atttcctgca atttaggttc gttgaatatt
     3541 gcgcacacca tggattcccc cgatttttcc cgcacggtag agactgccgt gcgcggttta
     3601 acggcggtgt cagatatgag tcatatccgc agcgtgccgt ccatcgaagc cggaaatgcc
     3661 gcctcgcacg ccatcggact ggggcagatg aatttacatg gttatctggc gagggaaggc
     3721 atcgcttatg gttcgccgga agccctggat ttcaccaatc tctatttcta taccatcacc
     3781 tggcacgcac tgcgtacctc gatgttgctg gcgcgcgaac gcggtgaaac cttcgccggg
     3841 ttcaaacagt cacgctatgc cagtggtgaa tatttcagcc aatatctgca aggtaactgg
     3901 cagccgaaaa cggcgaaagt tggcgaactg tttgcccgca gctgtattac gttacctacc
     3961 cgtgagatgt gggcgcaact gcgcgacgac gtgatgcgct acggcatata caaccagaat
     4021 ctccaggcgg tgccgccaac cgggtctatc tcttatatca accatgctac gtcgagtatt
     4081 catccgattg tggcgaaagt agagatacgc aaagagggca aaacaggacg cgtttactac
     4141 cctgccccgt ttatgactaa cgaaaatctg gcgctgtatc aggacgctta cgaaattggc
     4201 gcagaaaaga tcatcgacac ctacgcggaa gcgactcgcc atgtcgatca ggggctgtcg
     4261 ctgacgctgt ttttccccga taccgccacc actcgcgata tcaacaaagc gcagatttac
     4321 gcctggcgca aaggtatcaa aacgctctat tacatccgcc tgcgtcagat ggcgctggaa
     4381 ggcactgaaa ttgaaggctg cgtctcctgt gcactttaag gaatatctat gaaactctca
     4441 cgtatcagcg ccatcaactg gaacaagata tctgacgata aagatctgga ggtgtggaat
     4501 cgcctgacca gcaatttctg gctaccagaa aaggtgccgc tgtcgaacga tattcccgcc
     4561 tggcagacat taactgtcgt agaacaacaa ctgacgatgc gcgtttttac tggcctgacg
     4621 ctgctcgaca cgctgcaaaa tgttatcggc gcgccttcgc tgatgcccga tgcgctcacg
     4681 cctcatgaag aggcggtatt atcgaatatc agctttatgg aagcggttca tgcccgctct
     4741 tacagttcga ttttctcgac gctgtgccag accaaagatg tcgatgccgc ctacgcctgg
     4801 agtgaagaaa acgcaccgtt gcagcgaaaa gctcagatta ttcagcaaca ttatcgcggt
     4861 gatgatccgc tgaaaaagaa aatcgccagt gtgtttcttg aatctttttt gttctattcc
     4921 ggtttctggc tgccgatgta tttttccagc cgcggaaagc tgaccaatac cgcggacctg
     4981 atccgtctga ttatccgcga tgaagcagtc cacggttact acataggcta taaatatcag
     5041 aaaaacatgg aaaagatatc tctgggacaa cgtgaagagt tgaagagttt cgccttcgat
     5101 ttgttgctgg aactctacga caacgagttg caatacaccg atgagctgta cgccgaaacc
     5161 ccgtgggctg acgatgtgaa agcgtttctc tgttacaaca ccaataaggc tttgatgaat
     5221 ctgggctacg aaccgttatt tcccgcagaa atggcggaag tgaatccggc aatcctcgcc
     5281 gcgctttcgc cgaatgccga tgaaaatcac gatttctttt ccggttcagg ctcctcttat
     5341 gtgatgggga aagcggttga aacagaagat gaagactgga atttctgagg gtgttatttt
     5401 caaaaatatc actacccgca gcagggaaat aattcccgcc aaatagcttt ttatcacgca
     5461 aatattctgt agtgatctac actgatactc tgttgcatta ttcgcctgaa actacaatat
     5521 tcaggcgttt tttcgctatc tatgacaaaa aatatcaact tttctcgatt tgctctcagc
     5581 ccttatatca cggaaaattc cggcgatttg ctcgcatcaa tattcatgcc acatttgccc
     5641 tcaggggttg cctcagattc tcagtatgtt agggtagaaa aaagtgacta ttcccattgg
     5701 gtaatatatc gacataaaca aataaagaaa tcattctatt gcatggccat taaattaaaa
     5761 attaaaaatc tttataaaat atttggtgag catccacagc gagcgttcaa atatatcgaa
     5821 caaggacttt caaaagaaca aattctggaa aaaactgggc tatcgcttgg cgtaaaagac
     5881 gccagtctgg ccattgaaga aggcgagata tttgtcatca tgggattatc cggctcgggt
     5941 aaatccacaa tggtacgcct tctcaatcgc ctgattgaac ccacccgcgg gcaagtgctg
     6001 attgatggtg tggatattgc caaaatatcc gacgccgaac tccgtgaggt gcgcagaaaa
     6061 aagattgcga tggtcttcca gtcctttgcc ttaatgccgc atatgaccgt gctggacaat
     6121 actgcgttcg gtatggaatt ggccggaatt aatgccgaag aacgccggga aaaagccctt
     6181 gatgcactgc gtcaggtcgg gctggaaaat tatgcccaca gctacccgga tgaactctct
     6241 ggcgggatgc gtcaacgtgt gggattagcc cgcgcgttag cgattaatcc ggatatatta
     6301 ttaatggacg aagccttctc ggcgctcgat ccattaattc gcaccgagat gcaggatgag
     6361 ctggtaaaat tacaggcgaa acatcagcgc accattgtct ttatttccca cgatcttgat
     6421 gaagccatgc gtattggcga ccgaattgcc attatgcaaa atggtgaagt ggtacaggtc
     6481 ggcacaccgg atgaaattct caataatccg gcgaatgatt atgtccgtac cttcttccgt
     6541 ggcgttgata ttagtcaggt gttcagtgcg aaagatattg cccgccggac accgaatggc
     6601 ttaattcgta aaactcctgg cttcggccca cgttcggcac tgaaattatt gcaggatgaa
     6661 gatcgcgaat atggctacgt tatcgaacgc ggtaataagt ttgtcggcgc agtctccatc
     6721 gattcgctta aaaccgcgtt aacgcagcag caaggtcttg atgcggcgct gattgatgcg
     6781 ccgttagcag tcgatgcaca aacgcctctt agcgagttgc tctctcatgt cggacaggca
     6841 ccctgtgcgg tgcccgtggt cgacgaggac caacagtatg tcggcatcat ttcgaaagga
     6901 atgctgctgc gcgctttaga tcgtgagggg gtaaataatg gctgatcaaa ataatccgtg
     6961 ggataccacg ccagcggcgg acagtgccgc gcaatccgca gacgcctggg gtacaccgac
     7021 gactgcaccg gctgacggcg gtggtgctga ctggctgacc agtacgcctg cgccaaacgt
     7081 cgagcatttt aatattctcg atccgttcca taaaacgctg atcccgctcg acagttgggt
     7141 cactgaaggg atcgactggg ttgttaccca tttccgtccc gtcttccagg gcgtgcgcgt
     7201 tccggttgat tatatcctca acggtttcca gcaattgctg ctgggtatgc cagcgccagt
     7261 ggcgattatc gttttcgctc tcatcacctg gcagatttcc ggggtcggaa tgggcgtggc
     7321 gacgctggtc tcgctgattg ccatcggcgc aatcggtgcc tggtcgcagg caatggtgac
     7381 tctggcgctg gtgttaactg ccctgctgtt ctgtatcgtc atcggtttgc cgttggggat
     7441 atggctggcg agaagtccgc gagcggcgaa aattattcgt ccactgcttg atgccatgca
     7501 gaccacgcca gcgtttgttt atctggtgcc aatcgtcatg ctatttggta tcggtaacgt
     7561 gccgggcgtg gtggtgacga tcatctttgc gctgccgccg attatccgtc tgaccattct
     7621 ggggattaac caggttccgg cggatctgat tgaagcctcg cgctcattcg gtgccagccc
     7681 gcgccagatg ctgttcaaag ttcagttacc gctggcgatg ccgaccatta tggcgggcgt
     7741 taaccagacg ctgatgctgg ccctttctat ggtggtcatt gcctcgatga ttgccgtcgg
     7801 cgggctgggt cagatggtac ttcgcggtat cggtcgtctg gatatggggc ttgccaccgt
     7861 tggcggcgtc gggattgtga tcctcgccat tatcctcgat cgcctgacgc aggccgttgg
     7921 gcgcgactca cgcagtcgcg gcaaccgtcg ctggtacacc actggccctg ttggtctgct
     7981 gacccgccca ttcattaagt aactctgcac ttgcccggtg acgccgggca ttatcaccct
     8041 gccaaaaaaa ggaataacaa tgcgacatag cgtacttttt gcgacagcgt ttgccacgct
     8101 tatctctaca caaacttttg ctgccgatct gccgggcaaa ggcattactg ttaatccagt
     8161 tcagagcacc atcactgaag aaaccttcca gacgctgctg gtcagtcgtg cgctggagaa
     8221 attaggttat accgtcaaca aacccagcga agtggattac aacgttggct acacctcgct
     8281 tgcttccggc gatgcaacct tcaccgccgt gaactggacg ccactccatg acaacatgta
     8341 cgaagctgcc ggtggcgata agaaatttta tcgtgaaggg gtatttgtta acggcgcagc
     8401 acagggttac ctgatcgata agaaaaccgc cgaccaatac aaaatcacca acatcgcaca
     8461 actgaaagat ccgaagatcg ccaaactgtt cgataccaac ggcgacggta aagcggattt
     8521 aaccggttgt aaccctggct ggggctgcga aggtgcgatc aaccaccagc ttgccgcgta
     8581 tgaactgacc aacaccgtga cgcataatca ggggaactac gcagcgatga tggccgacac
     8641 catcagtcgc tacaaagagg gcaaaccggt gttttattac acctggacgc cgtactgggt
     8701 gagtaatgag ctgaagccag ggaaagatgt ggtctggttg caggtgccgt tctccgcact
     8761 gccgggcgat aaaaatgccg ataccaaact gccgaatggc gcgaattacg gcttcccggt
     8821 cagcaccatg catatcgttg ccaacaaagc ctggaccgag aaaaatccgg cggcagccaa
     8881 actgtttgcc attatgcagt tgccagtggc cgatattaac gcccagaacg ccattatgca
     8941 tgacggcaaa gcctcagaag gcgatattca gggccacgtt gatggctgga tcaaagccca
     9001 ccagcagcag ttcgatggct gggtgaatga ggcgctggca gcgcagaagt aatttttatt
     9061 cgggcggata aggcgttttc gcctcatccg ccgctctgta cacaatgcct gatgcgacgc
     9121 tgacgcgtcc tatcatgcct acaaaccaca tcgcacattt caacaagcta tctttcatcc
     9181 catattcatc aacatccgct attattgatt tccagcttaa tcatcacctg atgaacaaaa
     9241 ataatgacta aacctaatca tgagcttagc ccggcgctga tcgtgctgat gtctatcgcc
     9301 accggtctgg cggtagccag taactattac gcccagccat tgctcgacac catcgcgcgt
     9361 aacttttccc tttccgccag ttcggcaggc tttattgtta ccgccgcgca gttgggctat
     9421 gccgcaggtc tactgtttct tgttcccctc ggtgatatgt ttgaacgccg ccgcctgatt
     9481 gtctcgatga ccttactggc agcgggcggt atgttaatca ctgccagcag tcagtcactg
     9541 gcgatgatga tcctcggtac ggcattaacc ggtttattct cagtcgtggc acaaattctg
     9601 gttccgctgg cagcgacgct ggcttcaccg gataaacgcg gcaaagtggt tggcaccatt
     9661 atgagcggtc tgctgttggg gatattgctg gcacgaaccg ttgccgggtt actggcaaac
     9721 ctcggcggct ggcgcaccgt cttttgggtt gcttcggtgt taatggcact gatggcgctg
     9781 gcattatggc gtggtctgcc acaaatgaaa tcagaaaccc acctcaacta cccacagttg
     9841 ttgggttccg ttttcagcat gttcatcagc gataagatcc tgcgcacccg cgcgttgctg
     9901 ggctgcatga cctttgccaa cttcagcatt ctctggacct caatggcctt tttgcttgcc
     9961 gctccacctt ttaactacag cgatggtgac attggtctgt ttgggcttgc gggagctgcc
    10021 ggagcgttgg gcgctcgtcc ggcgggcggt tttgccgaca agggcaaatc gcaccacaac
    10081 tttcggtctg cttctgctgt tactttcatg gctggcaatc tggtttggtc acacttccgt
    10141 actggcgttg attatcggca ttctggtgct ggacctcacc gtacagggcg tgcatatcac
    10201 taaccagacg gtaatttatc gaatacatcc tgatgcgcgt aatcgcctga ccgcaggtta
    10261 catgaccagc tactttattg gcggtgccgc cggttcgcta atttcagcct cagcctggca
    10321 acatggcggt tgggctggcg tttgtctggc tggcgcgacg attgccctgg ttaacttact
    10381 ggtctggtgg cgaggttttc atcgtcagga agccgcaaat taagcaatca gcaaccgttt
    10441 tcgtggcttt acacttataa gggtgttaag aagcccatca gtctgataag gttaagatat
    10501 tcattcagtc tatttataat attaacaatc gttaagcgta cactctatgg aaagccctac
    10561 tccacagcct gctcctggtt cggcgacctt catggaagga tgcaaagaca gtttaccgat
    10621 tgttattagt tatattccgg tggcctttgc gttcggtctg aatgcgaccc gtctgggatt
    10681 ctctcctctc gaaagcgttt ttttctcctg catcatttat gcaggcgcga gccagttcgt
    10741 cattaccgcg atgctggcag ccgggagtag tttgtgggtt gctgcactga gcgtcatggc
    10801 aatggatgtt cgccatgttt tgtatggccc gtcactgcgt agccgtatta ttcagcgtct
    10861 gcaaaaatcg aaaaccgccc tatgggcgtt tggcctgacg gatgaggttt ttgccgccgc
    10921 caccgcaaaa ctggtacgca ataatcgccg ctggagcgag aactggatga tcggcattgc
    10981 cttcagttca tggtcatcgt gggtatttgg tacggtaata ggggcattct ccggcagcgg
    11041 cttgctgcaa ggttatcccg ccgttgaagc tgcattaggt tttatgcttc cggcactctt
    11101 tatgagtttc ctgctcgcct ctttccagcg caaacaatct ctttgcgtta ccgcagcttt
    11161 agttggtgcc cttgcaggcg taacgctatt ttctattccc gtcgccattc tggcaggcat
    11221 tgtctgtggc tgcctcactg cgttaatcca ggcattctgg caaggagcgc ccgatgagct
    11281 atgaggttct gctgcttggg ttactggttg gcgcggcgaa ttattgcttc cgctatttgc
    11341 cgctgcgcct gcgtgtgggt aatgcccgcc caaccaaacg tggcgcggta ggtattttgc
    11401 tcgacaccat tggcatcgcc tcgatatgcg ctctgctggt tgtctctacc gcaccagaag
    11461 tgatgcacga tacacgccgt ttcgtgccca cgctggtcgg cttcgcggta ctgggtgcca
    11521 gtttctataa aacacgcagc attatcatcc caacactgct tagtgcgctg gcctatgggc
    11581 tcgcctggaa agtgatggcg attatataac ccacaagaat catttttcta aaacaataca
    11641 tttactttat ttgtcactgt cgttactata tcggctgaaa ttaatgaggt catacccaaa
    11701 tggatagttc gtttacgccc attgaacaaa tgctaaaatt tcgcgccagc cgccacgaag
    11761 attttcctta tcaggagatc cttctgactc gtctttgcat gcacatgcaa agcaagctgc
    11821 tggagaaccg caataaaatg ctgaaggctc aggggattaa cgagacgttg tttatggcgt
    11881 tgattacgct ggagtctcag gaaaaccaca gtattcagcc ttctgaatta agttgtgctc
    11941 ttggatcatc ccgtaccaac gcgacgcgta ttgccgatga actggaaaaa cgcggttgga
    12001 tcgaacgtcg taaaagcgat aacgatcgcc gctgcctgca tctgcaatta acggaaaaag
    12061 gtcacgagtt tttgcgcgag gttttaccac cgcagcataa ctgcctgcat caactctggt
    12121 ccgcgctcag cacaacagaa aaagatcagc tcgagcaaat cacccgcaaa ttgctctccc
    12181 gtctcgacca gatggaacaa gacggtgtgg ttctcgaagc gatgagctaa cgcgtcatct
    12241 cgctcaaaaa tccagattta taaaagaaaa aatgaccggc cagcatcgca acatgctggc
    12301 ctttttggca agcaggtcgg ctcagccgat gagttaagaa gatcgtggag aacaatatga
    12361 gcgcaaatgc ggagactcaa accccgcagc aaccggtaaa gaagagcggc aaacgtaagc
    12421 gtctgctcct ccttctcacc ttgctcttta taattattgc cgtagcgata gggatttatt
    12481 ggtttttggt actgcgtcat ttcgaagaaa ccgatgacgc atacgtggca gggaatcaga
    12541 ttcaaattat gtctcaggtg tctggcagcg tgacgaaagt ctgggccgat aacaccgatt
    12601 ttgtaaaaga aggcgatgtt ctggtcactc tcgacccgac agatgctcgc caggcgtttg
    12661 aaaaagccaa aactgcactg gcttccagcg ttcgccaaac ccaccagctg atgattaaca
    12721 gcaaacagtt gcaggcgaat attgaggtgc aaaaaatcgc cctcgcgaaa gcacaaagcg
    12781 actacaaccg ccgtgtgccg ctgggcaatg ccaacctgat tggtcgcgaa gagctgcaac
    12841 acgcccgcga cgccgtcacc agtgcccagg cgcaactgga cgtcgctatt caacaataca
    12901 atgccaatca ggcgatgatt ctggggacta aactggaaga tcagcctgcc gtgcaacagg
    12961 ctgccaccga agtacgtaac gcctagctgg cgctggagcg tacccgtatt gtcagtccga
    13021 tgaccggtta tgtttcccgt cgtgccgtgc aaccgggtgc gcaaattagc ccaacgacgc
    13081 cgctgatggc ggtcgttcca gccaccaata tgtgggtgga tgccaacttt aaagagacgc
    13141 agattgccaa tatgcgcatc ggtcagccgg tcaccatcac caccgatatt tacggcgatg
    13201 atgtgaaata caccggtaaa gtggttggtc tggatatggg cacaggtagc gcgttctcac
    13261 tgcttccggc gcaaaatgcg accggtaact ggatcaaagt cgttcagcgt ctacctgtgc
    13321 gtatcgaact ggaccagaaa cagctggagc aatacccgct gcgcattggt ttgtccacgc
    13381 tggtgagcgt caataccact aaccgtgacg gtcaggtact ggcaaataaa gtacgttcaa
    13441 caccggtcgc ggtaagtacc gcgcgtgaaa tcagcctggc acctgtcaat aaactgatcg
    13501 acgatatcgt aaaagctaac gctggctaat ccagaggtgc gtgtgatgca acagcaaaaa
    13561 ccgctggaag gcgcgcaact ggtcattatg acgattgcgc tgtcactggc gacattcatg
    13621 caggtgctga actccaccat tgctaacgtg gcgatcccca ctatcgccgg gaatctgggc
    13681 tcatcgctca gccaaggaac gtgggtaatc acttctttcg gggtggcgaa tgccatctcg
    13741 atcccgctta ccggctggct ggcaaagcgc gtcggggaag tgaaactgtt cctttggtcc
    13801 accatcgcct ttgctattgc gtcttgggcg tgtggtgtct ccagcagcct gaatatgctg
    13861 atcttcttcc gcgtgattca ggggattgtc gccgggccgt tgatcccgct ttcgcaaagt
    13921 ctattgctga ataactaccc gccagccaaa cgctcgatcg cgctggcgtt gtggtcgatg
    13981 acggtgattg tcgcgccaat ttgcggcccg atcctcggcg gttatatcag cgataattac
    14041 cactggggct ggatattctt catcaacgtg ccgattggcg tggcggtggt gttgatgaca
    14101 ctgcaaaccc tgcgcggacg tgaaacccgc accgaacggc ggcggattga tgccgtgggg
    14161 ctggcactgc tggttattgg tatcggcagc ctgcagatta tgctcgaccg cggtaaagag
    14221 ctggactgtt tttcatcaca ggaaattatc attcttaccg tggtggcggt ggtggctatc
    14281 tgcttcctga ttgtctggga gctgaccgac gataacccga tagtcgatct gtcgttgttt
    14341 aagtcgcgca acttcaccat cggctgcttg tgtatcagcc tcgcgtatat gctctacttc
    14401 ggcgctattg ttctgctgcc gcagttgttg caggaggtct acggttacac ggcgacctgg
    14461 gcaggtttgg cctctgcgcc ggtagggatt attccggtga ttctgtcgcc gattatcggc
    14521 cgcttcgcgc ataaactgga tatgcggcgg ctggtaacct tcagctttat tatgtatgcc
    14581 gtctgcttct actggcgtgc ctataccttt gaaccaggta tggatttttg cgcgtcggcc
    14641 tggccgcagt ttatccaggg gtttgcggtg gtctgcttct ttatgccgct gaccaccatt
    14701 acgctgtctg gtttgccacc ggaacgactg gcggcggcat cgagcctctc taactttacg
    14761 cgaacgctgg cggggtctat cggcacgtcg ataaccacga ccatgtggac caaccgcgag
    14821 tcgatgcacc atgcgcagtt gactgagtcg gtaaacccgt tcaacccgaa tgcccaggcg
    14881 atgtacagtc aactggaagg gcttgggatg acgcaacagc aggcatcagg ctggattgcc
    14941 cagcagatca ccaatcaggg gctgattatt tccgccaatg agatcttctg gatgtcagcc
    15001 gggatattcc tcgtactgct ggggctggtg tggtttgcta aaccgccatt tggcgcaggt
    15061 ggcggcggag gcggtgcgca ctaagtacaa ctaagccagt tcatttgaac tggctttttt
    15121 caattaattg tgaagatagt ttactgacta gatgtgcagt tcctgcaact tctctttcgg
    15181 cagtgccagt tcttcgttgc tgttgatgcg tacctcacgt tccagaatgc tacgcgcaat
    15241 atcctgcgct tcctgcaacg agtgcatctg gtaagtgcca cactggtaga cgttcagttc
    15301 cgggatctga ttctgatcct gcactttcag cacgtcttcc attgccgctt tccaggcatc
    15361 agcaacacgc tgctcatctg gcgtaccaat cagactcata taaaaaccgg tgcggcagcc
    15421 cattggcgag atatcgataa tctctacacc attaccgtta agatggttac gcataaaacc
    15481 agcaaacagg tgctccaggg tatggatccc tctttctggc atcacttctt tgttcggcac
    15541 gcagaagcgc agatcgaaca cggtgattgc gtcgccatgc ggggtgttca ttgttttcgc
    15601 cacccgaact gcaggcgctt ccatccgggt atgatcgact gtgaagctat ctaacaacgg
    15661 catttagcca cctccggtaa tttttttaaa aattttctga actctttctt cccaggcgag
    15721 tctgagtata tgaaagacgc gcatttgtta tcatcatccc tgaattcaga gatgaaattt
    15781 tggccactcc cgagtggcct ttttcttttc tgtcaggcgt gtttttccag ccacaccgca
    15841 aacggttcgg tatcagcggt ttccatttcc tgctgacggc gttcagatgc ctcgcgctcg
    15901 gctacaaaat cctcttcgcg cagaatttcc agcggctctt cacgcagcag attacggtag
    15961 gcttctgcaa acgctttgcc tgttccgcca ataccagtat caatcataga ccttaagata
    16021 cgggcagaga aagtcagatc gggattatcg aagcaggcaa ccagttcatc acacactttc
    16081 tgatacgctt cgccgccgtt aatgctatcc agcgtttgcg cgacgcgttt cagatcgcgg
    16141 aacagatctt tacccacctg cagtaacggg aactgcgcgg tttcgcagcc gatacccagc
    16201 gtcaggcccg gtttgcgacc ttcaaggatc acccggttcc agttaacgcg cgtacaggca
    16261 agttcgctac tgctcatttc cggtgcatca gccagcgcac accagaccat aaacaggtcg
    16321 aggaatcgca cctgctgttc atctacacca atcggcgaga acgggttgat gtccagcgaa
    16381 cgcacttcaa tatattcaat gccgccacgt aacagcgcat cagaaggtga ctcgccgctg
    16441 cgggtaacgc gttttggacg aatcggcgca tacagttcgt tttcaatctg cagcacgttg
    16501 ctattgattt gcagcctctt accgtctttc tcaataccaa tcttcgcgta ctcttccgat
    16561 ggcgttttga ttgcctgttt aaggcccgct acgtactcgt aaagatcgtt gaaggtaata
    16621 ccaagattgc tttgcgattt attggtatag ccgagatcgc tcaaacgaag agaggtcgca
    16681 tacggcaggt aatacatacc gcactcggtt ttctcaaacg gcagcgacgt tggttttcct
    16741 tgcaggaaag aagaacaaat cgccggagat gcaccaaaca gataaggaat aacccaaccg
    16801 aaacgatagt aattgcggat aacgcggaaa tagcccgcag aaattttctc tttggcatca
    16861 gcgcccgaga tatcaccgca cttcgcttgc cagaatgcca ttggcaaaga gaaattgtag
    16921 tgcacgccgg aaatggtttg catcagcgcg ccgtagcgat ttttcagccc ttcacgatac
    16981 agcgttttaa agcgtccggt gttagaagtg ccgtactgtg ccagttcgat gtcctgacct
    17041 tctgcgatgt agcatggcat acttaacggc cacatccgct catcgcccat attgcgcgcc
    17101 gtataacgat gcagatcgcg cataaaggtc agcatatgtt caatatcacc atccactggt
    17161 gtaatgaatt ccagcaatgc ttccgcaaaa tcggtagtaa tccatttgtg cgtcagtgcg
    17221 gaacctaatg cttcaggatg acctgttgtt gccagtgtgc catcagcatt aacacgcaaa
    17281 gtttcgcgct ccagcccacg ctgtatcccc tttaacgcct gaggatgttt ttccagccag
    17341 gccagcgcct gtgatacgtc cgggatcaaa ttgacctccc gcctgtcaaa atcgttttaa
    17401 ttagcataac tgtaatggtg accatatgtg caggcctaca attagtgcca ccacatcatg
    17461 ccctgaacgg tcgctgctgc aactgcaaca tagcgtaacg ctttaccaag gcacaaaaaa
    17521 aagattactg gcccccagga gatgcgcatc catcccgcta acagacacag taaatcgcca
    17581 accacgggca tccagcttaa taatagcgtg actgcaccat agcgtttcag ccagccggta
    17641 gctttctctt gccagcgcga tgttttacgc aatggaaaga aacgcccaag gataacgtta
    17701 gttaaccctc caaggctatt acccattgtt gctgttaaaa ctaaaaccca gggatgactg
    17761 atcccggaaa gcaacattgc caccagcacg acttcggagt tgccgggtaa tagtgtagcg
    17821 ctgagaaaac tactggcaaa caacgagaaa agcgataacg cttcactcac agcaagcgaa
    17881 catccacagc gtccatgcct gctgcacggg ccgcctgaat accgaaatcg gcatcttcaa
    17941 agaccacaca ctgcgtcggt tgcacgccca tacgctgcgc gcacaacaaa aatgtgtctg
    18001 gcgcgggttt atggtgtttg acgtgatcag cagcgacgac ggcgtcaaaa taacggcgta
    18061 atcccaggtg cgccagcaat gcctcagcga tggcgctttc actccccgtt cctacagcca
    18121 ttgggcgacg accatgccaa cttttcacca cttcaacaag aggaagcggc tcgacactat
    18181 ccagcagcat acttcttact gcttctgttt tttcacgcgc taacgcatgc gggtcgagat
    18241 cggcctgatt cagctcaata attgcctgag caatacgcca ggtgggcgat ccattaagcg
    18301 caatcatcgc ctgaatatcg tactgaagac cgtagtgccc taatacttcg cgccacgctt
    18361 tacggtgcgt aggcgccgta tccaggattg tgccatccat atcaaaaatt aaacctgcat
    18421 aacgctcgta catggtcttc tcgcaagtcg aaaaatatga cgctacttta gcgtaattgc
    18481 ttgattttgt cgctgatggc gaaggagaag aaaattcagg aaatggataa agtagttatt
    18541 tagaaatgag atatttttga aggaaatttt ttggagaa
//
LOCUS       DAZPCS010000070        18215 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZPCS010000070 DAZPCS010000000
VERSION     DAZPCS010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18215
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.070"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(61..819)
                     /gene="aroD"
                     /locus_tag="ACHUQ7_002307"
     CDS             complement(61..819)
                     /gene="aroD"
                     /locus_tag="ACHUQ7_002307"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFR1135694.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLVGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(850..1716)
                     /gene="ydiB"
                     /locus_tag="ACHUQ7_002308"
     CDS             complement(850..1716)
                     /gene="ydiB"
                     /locus_tag="ACHUQ7_002308"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFR1135695.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTDTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1728..3000)
                     /locus_tag="ACHUQ7_002309"
                     /pseudo
     CDS             complement(1728..3000)
                     /locus_tag="ACHUQ7_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(3183..4388)
                     /locus_tag="ACHUQ7_002310"
     CDS             complement(3183..4388)
                     /locus_tag="ACHUQ7_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1135696.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFDILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSTGSIATFTIPLITAHLSQRSIADIMWFDTAIGFLLALFIGLRSRKKTRHHS
                     LKENVAPGG"
     gene            complement(4488..4844)
                     /locus_tag="ACHUQ7_002311"
     CDS             complement(4488..4844)
                     /locus_tag="ACHUQ7_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFR1135697.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(4980..5087)
                     /gene="rprA"
                     /locus_tag="ACHUQ7_002312"
     ncRNA           complement(4980..5087)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHUQ7_002312"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5273..6385)
                     /gene="ydiK"
                     /locus_tag="ACHUQ7_002313"
     CDS             complement(5273..6385)
                     /gene="ydiK"
                     /locus_tag="ACHUQ7_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFR1135698.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6774..9830
                     /locus_tag="ACHUQ7_002314"
     CDS             6774..9830
                     /locus_tag="ACHUQ7_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFR1135699.1"
                     /translation="MIPQISLAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDMPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9827..10237
                     /gene="menI"
                     /locus_tag="ACHUQ7_002315"
     CDS             9827..10237
                     /gene="menI"
                     /locus_tag="ACHUQ7_002315"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFR1135700.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10337..10525
                     /locus_tag="ACHUQ7_002316"
     CDS             10337..10525
                     /locus_tag="ACHUQ7_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFR1135701.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11073..11441
                     /gene="sufA"
                     /locus_tag="ACHUQ7_002317"
     CDS             11073..11441
                     /gene="sufA"
                     /locus_tag="ACHUQ7_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFR1135702.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFRV"
     gene            11450..12937
                     /gene="sufB"
                     /locus_tag="ACHUQ7_002318"
     CDS             11450..12937
                     /gene="sufB"
                     /locus_tag="ACHUQ7_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFR1135703.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12947..13693
                     /gene="sufC"
                     /locus_tag="ACHUQ7_002319"
     CDS             12947..13693
                     /gene="sufC"
                     /locus_tag="ACHUQ7_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFR1135704.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13668..14939
                     /gene="sufD"
                     /locus_tag="ACHUQ7_002320"
     CDS             13668..14939
                     /gene="sufD"
                     /locus_tag="ACHUQ7_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFR1135705.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14936..16156
                     /gene="sufS"
                     /locus_tag="ACHUQ7_002321"
     CDS             14936..16156
                     /gene="sufS"
                     /locus_tag="ACHUQ7_002321"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFR1135706.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGQQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVE
                     RLVTGLQRIHRLLG"
     gene            16169..16585
                     /gene="sufE"
                     /locus_tag="ACHUQ7_002322"
     CDS             16169..16585
                     /gene="sufE"
                     /locus_tag="ACHUQ7_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFR1135707.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDGSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16734..17738
                     /gene="ldtE"
                     /locus_tag="ACHUQ7_002323"
     CDS             16734..17738
                     /gene="ldtE"
                     /locus_tag="ACHUQ7_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFR1135708.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17801..18037)
                     /gene="lpp"
                     /locus_tag="ACHUQ7_002324"
     CDS             complement(17801..18037)
                     /gene="lpp"
                     /locus_tag="ACHUQ7_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFR1135709.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT         4789 a         4403 c         4679 g         4344 t
ORIGIN      
        1 cggttaaaaa tacttttttt atttcggcgg gggagggtat ttccgccgaa atattattgc
       61 ttatgcctga tgtaaaatag ttaataccgt gcgcaaatca tttaccgaga tttgccctgg
      121 cgcagaggct ttttttaccg caccaaaagt tgccgccgag ccaaatactt caccaaccag
      181 acgagaaatt acgccagttt ttgccatcga catcgtgatg attggacgat cggcatactg
      241 ctcctgcatc tccagggtcg cggcaagcaa cgtcagcaca tcgctggtac tttgcggcat
      301 cagcgcaatc ttaggaatat cggcgtcgaa ggactgcatt ttgcgcagac gggcaatgat
      361 ttcttcggct tccggcgttt tatggaagtc atggttggac atgacaactt tcacatcatg
      421 cgcgtgggcg taggcgacgg tttctttgac ctgatcatcg ccggtaaata actccagatc
      481 gatcatatca accaggccgc tgtcgatggc tgcacgattg agagcaatat aagcctcggt
      541 ggaaatcgcc tgctcgccgc cttctttggc actgcggaag gtaaacagca gcggtttttc
      601 tggcatggtt tcacggagaa tttttgccgc cgccatgaca gactccacat tggagaggtc
      661 ggcaaagtgg tccacacgcc attccagaat atcaaagtcc gcttcacgat aggcgagagc
      721 ttcggatttc acgcgggcga tatctttcgc catcagcgag acgatgattt taggtgcgcc
      781 cgcaccaatg acgagatctt ttacagttac ggttttcatt ttttaccctt tctgcacgcg
      841 gtcagcctgt caggcaccga accccatgac ctgtttaaca tattccagag ggaaatcttt
      901 gccagtccat aacgtgaact gttcagcccc ttgccacaac aacatgccgt atccatcaat
      961 cgttttgcaa ccagcttgtt gtgcctgctg caataacttc gtcatatgcg ggttatacac
     1021 gcattcagtg accagcagtc ccggatgtaa cagactgata tcattaacca atgattcatt
     1081 ctcaaggggt ttcataccta cttttgtgcc attggttaaa atgtcggcgg aagccagggc
     1141 ttcagcaaag gcttgctgat cggcgagatc ggtatccgtg acgacacaat cggtgttttc
     1201 gttaacccgc tgcgcgaagg cgagggcttt atcgaagaac tcatcccgac ggttaaagag
     1261 tttaatttct tttaaacctt caattgcccc ctgcgcgcca atcgccgttg aggcacctcc
     1321 ggcccctaac agcaccatcg ttttgccttt gatatcaaaa ccgctctctt taatggcgcg
     1381 aatatggccc gtgccgtcgg tgttatagcc acgcagatag ccatcatcat taacgatggt
     1441 gttgatggca cccaccagtt tggctgctgg cgttaattca tcaacatatt cacacgccag
     1501 ttgtttgttc ggcatcgaca caccagttcc gcgcattttg agggctttta atccttcaat
     1561 tgctccggga aagctatcgt tatccacttc gaaggccata taggtaaatg gcaatcccgc
     1621 tttttctaag gctttattct gcatttcagg cgataaactg tggcggatag gataagccat
     1681 caatccaatc aattcgtatt ttgcggtaac atccataatt aactccttta acctctatgc
     1741 ttaattgtgt ttaaccgggt gctaaatttg cgttcgccaa accgcacatc attctcagga
     1801 ataataaaaa cgcggtaata gcggctaaaa acaataattg cggtaatcag ggccagcagc
     1861 gcgaaagtaa aatcgagaac aatgatatat tgcaggccga tgttcgacag ataaccggta
     1921 atcagcggaa taacaaagtt agccagtcca cccatcatca tataaatact ggtgactttg
     1981 gctttgcttt tggggaaaaa ctctgacatg accgaaacac cgagctgtaa aatgccgcca
     2041 gctgctgaga aaccgataac aaaggcgccg gcattacaca ccagtggaga agggtacagg
     2101 tagataatgg cagctgttat tgttgccagc gcagagttaa atacattagc ccagatgggc
     2161 cggaccattt ttttcagtag tgcggcaaaa ataaagacac agaccaacga gcccatactg
     2221 taataagaga tggtttttaa tgcctcagct tctgacatac cagcaaaagc catcgcatat
     2281 ttgggcatcc acaccacaat cacataaaag gtcgagaatg cggctacacc gaacagtacc
     2341 gatgaaacac cttccagcca gactaaaggt tttgctgttc atttgcggta attcattagc
     2401 tacgctggcg tccaccaact ggctggggaa tttgcttttc aacagcatca gcgtgatcag
     2461 tacaaacaga atacccggaa taatcagccc atagccgtac cagatattat tgagcaacat
     2521 atagctcacc agcattgggt agaacatttg cccaaatgac accatcgctt taaccagtat
     2581 gaccgccgaa ccagaggctt tcggaaagca ttccatgagc gcggggtagc cacccgtatc
     2641 cagcgctgag ttagcgatac ctacgcacac tgccagaccg taggcgagag ttaaattcgg
     2701 gcaagcggga ataccaaaga agaatagcag atacattatt actgccatta atatcaccgc
     2761 ccgacgacca aacttatcgg agatcacacc gaagaataaa atactgatca atcgccccaa
     2821 accgataccg gaaattaaat aggcaatgcc cgcgttgtca gtggaaaact tttctgacag
     2881 agatgtcata ttttgggcaa gcgtaataac actaatgccg tgcaggaaat agctgaagta
     2941 aatacaaaga acagccagga taaatggcgt gctgaaagat aatttcgtta ccgtacgtat
     3001 tgttctccga cggtttacat aattactata ctgaagatga ttttccctgg gtacatggca
     3061 taaagtttat gtttccagga atattgcatg tcatagtttg tttttgatcg cgattttcct
     3121 tagttagcgc agaaagagtt atttatccgt aagatcttgc atgacctgaa aagaatattg
     3181 cattacccac ccggagcgac attttccttt agcgagtgat gccgcgtttt tttgcggctg
     3241 cgtaagccga taaacagtgc cagtaaaaaa ccgatggcag tatcgaacca cataatatcg
     3301 gcaatacttc tttgggacag atgagccgta atcaacggaa tagtaaaggt cgcaatactg
     3361 cccgtactgt aatagatccc tgtagcttta cctttagcgt aagggaaacg ttcagccatt
     3421 aacgtcaggc caatttgcac aacacctcca gcagaggtaa aaccaatgac aaaagcaaat
     3481 attatcacca cataaaatgt gggatgcagg cagacggtaa acagagcaat aaatgagata
     3541 aaggtgtaca gcatcagtaa tgttgttggg cgaacggtat tacgaatgag tggagcggta
     3601 ataaatacac aaagcagcga acccacggtg tagatactga gtagtttgat cgacatagtg
     3661 tatgacatgc ctgcaacaaa ttgtccgtac tgtgccagcc actggctaac cagataaaac
     3721 gttgccattg agatatagcc atataaggta taactggcta aatcgataat tgaacagcga
     3781 tgttccgtag agctggtggt tttctttatg acaggtaagc gacgacccgg atggggtggg
     3841 aacgtacaac ggtataaaaa cagagcgtta ataaacataa tgcctgcagc aatcataaag
     3901 gaccaaccga accacagttc agcccacact aacaggctaa tgattagcgg taataaaaat
     3961 tgtccgctgg aaacaaatgc tttaattaaa atattggctg tcccaggtga gcgtggaaaa
     4021 gcttccatca aactgggata agtgcctgca tcgagaaaac tgtttgccat tcccgccaga
     4081 aagccaaaaa cataagcgat aatgatgtta ttggtctgca ggatgtcaaa aaagaaggcc
     4141 atatagcagc acatcccgag catgataaaa gggcggcgac caaagcgatc ggataataat
     4201 cctgcaaaaa gcaagacact taatcgacca atgcccagcg atgagataac tatcgagaca
     4261 cccgcggcat tagtctgcca aagtgtctcc agcgaggcca tattcaggct catcaaaatg
     4321 acgcccatac catgcaccag gtaattaaaa tacaacccaa gtgcggtagg gtaataggga
     4381 tttttcatag gttctactta gcaaaggtgc tttaaaaagt gtagcgatgt tgtgtcagta
     4441 tccattcatt attgttgtaa caacgacaat tcctttctgt ggaaaaatta acaaagccgc
     4501 tccagatcgt gtgcatataa ttcagcggct accgattgat agatcttcca gtcaataaaa
     4561 ttgccatcag ggtagacttg ttgaaacgcg gttaagtcgg ggaaaaaacg catggtgttg
     4621 ttgccgatgc ggttatttat tttctctatg atggcatgaa gatgtttttt tcgttgctga
     4681 cgcatagcca acagttgctc gactacacaa tcaggaatgg cgcatttgcc attttcccac
     4741 tgacgccagc tttcactgtc accggtctgg gcaatccagg tcgcgcattc atcaatagtc
     4801 atggcaaaaa tatggcggag tgcctggagt tcataagcgt tcatcgtgta atggctgcat
     4861 gaaataaaca ataaaatata aataacatta tcagcctgct gacggcttga agagagtcac
     4921 agtatcttgt gcaacattat tggaaagagt gaggggcgag gtagcgaagc ggaaaaatgt
     4981 taaaaaaaag cccatcgtgg gagatgggaa aagactacac acagcaattc gttgtttcac
     5041 tcaggggatt tccatgctta taaatcaata tgttgattta taaccgtgag ctaatagtag
     5101 gcatacggac tttttgcgtc gatcagattc gtctcattag tttaaaaact gtaaagaatt
     5161 tgcgacaggt actaccagct gaatggcggt aagcaggaaa atatggggaa gagcaagcaa
     5221 atatcaatta gcggtgatta acaatcaggc ggcagagccg cctgatgaga aattacttat
     5281 tcggtttctc gatttcgcca agttcttcga gaatttcttc cggttggtcc gtcggtggcg
     5341 ggacttcttc cacccacgcg gcaaacagac gccaggaaac ggctaacaga accggaccaa
     5401 taaacagacc gatcatcccg aaagcaatca aaccaccaat aacgccagag aggatcagga
     5461 tcagcggtaa atcggcaccc atgcgaatta acattgggcg gatgacgtta tccagcgtgc
     5521 caaccacacc gctccacact aacaataccg ttccccaggt ggtatcgccg gtccagtaga
     5581 gccagataat cgccggaatc agtaccggca acgggccaag ctggacaagg caggagagga
     5641 tcattaacac cgttagcaac gttgcataag gtacgccgga tacggcgagg ccgataccgc
     5701 caagcactgc ctgtactaac gccgtcacca ccacacccag cgccaccgcg cggatagcct
     5761 gtgccgccag cagcacggcg gcatctccgc gaacgcctgc cagacgggtt gcaaaatggc
     5821 gaatgccttg tgccacctgt tcaccgcgcc agtacagcag ggcactgaag agaagcatca
     5881 gcgcacaatg caccataaag cgcccgatat gcgccgcctg cccaacgaac caggtggtgg
     5941 tggtgccaat ataagggcgg actttcgcca tgatcgccgt accccccata tccagcaagt
     6001 tgtgccagcc tgcatacagc ttcgcgccaa tcaccggaat ggtattaagc cacgccagat
     6061 cgggtaacgt catgtcgccg ctggaaatgg ctttaattag cgggccgctg ccgtcgacga
     6121 tactgttaac cagcaaagca atagggatga taaacaccat cactaataac agcgtcatca
     6181 ccagaacggc gagagagcgg cggccaaaca tgatcttttg caaacgtaac aataccggcc
     6241 aggtggcgat aaccaccgta ccggcccatg caaagccgag aataaagggt tgaacaatcc
     6301 acagacatgc cacaatcatg atggctaaaa acagcaccga aagcagaatt tgtgcgacat
     6361 ccctgggctg acgaacattt accataacta cttttcacct tttttgtgcg tcaaatcgtc
     6421 gacgcgacgt gaacacgcga aactcacctg cataatgatg agcaatttca gcggttttta
     6481 acaggccgat tctgccctta attctgatgg gcgtgttaga aaaaaatgtg gtacaacaac
     6541 tcaggcaaca cgcaaacgat ttactcgtcg tatttcaagt tgcatgtgct gcggctacgt
     6601 tcgttcaccc cagtcactta ctattgtaag ctcctggagt tcactcgctt gccgccttcc
     6661 tgcaactcga aatccataga gtaaataaac atcacctttc agtgcgctat tgcagccgcc
     6721 tgaaagaata gtgtatcgac ctacatcaca gaccgcagga aagggtcaat ataatgattc
     6781 cacagatttc cctggcaccc ggcgtcgttc aactggtgct taattttttg caagagctgg
     6841 agcaacaagg ttttaccggc gatgcggcga caagttatgc cgatcgtctg acaatgtcga
     6901 ccgacaacag tatttaccaa cttctccccg atgcggtggt atttccgcgt tcaaccgcag
     6961 atgtggcgct gatcgcccgt cttgccgcgc aggaacgcta ttcatcgctg atctttaccc
     7021 cccgcggcgg cggcaccggc actaacggtc aggcgctcaa ccaggggatt attgttgata
     7081 tgtcccgcca tatgaaccgc atcatcgaaa ttaaccctga agagggctgg gtgcgcgttg
     7141 aggccggggt gataaaagac caactcaatc agtacctgaa accgttcggc tacttttttg
     7201 cgccggaact ttcgaccagc aaccgggcaa cgctcggtgg gatgatcaat accgatgcat
     7261 ccggtcaggg atcgctggtc tatggcaaaa cgtcagatca cgtacttggc gtacgcgcgg
     7321 tgttgttggg gggcgatatt ctcgatacgc aacctttacc cgtcgagctg gcggaaacgc
     7381 tgggtaaatc caataccaca atcgggcgaa tttataacac ggtttatcaa cgttgccgtc
     7441 agcaacgcca gttaatcatc gacaacttcc ccaaacttaa ccgctttctt accggttacg
     7501 atctgcgtca tgtctttaac gatgagatga ccgagttcga cctgacgcgc attctgacgg
     7561 gttcagaagg gacgctggcc tttattaccg aagcgcggct ggatattacg cgcttgccta
     7621 aagtgcgccg tctggtgaat gtcaaatatg actcttttga ctctgcgctg cgtaacgcgc
     7681 cgtttatggt tgaggcgcgg gcgctttcgg tagagacggt ggactcaaaa gtgctgaatc
     7741 tggcgcggga agatattgtc tggcattccg tcagcgagtt gattaccgat atgcctgaca
     7801 aagagatgct cggactgaac attgtggaat ttgctggtga tgatgaggcg ctgattgacg
     7861 agcgagttaa tgcactctgt gtgcggcttg atgagctgat tgccagccaa caggcaggtg
     7921 tgattggctg gcaggtgtgc cgcgagctgg cgggggttga acgtatctat gcgatgcgca
     7981 aaaaagccgt tggtctgctt ggcaatgcca aaggtgccgc taagccaatt ccgtttgctg
     8041 aggatacctg cgtaccgccg gaacatctgg ccgattatat tgctgaattt cgcgcgctgc
     8101 tcgacagcca cggcttaagc tacggtatgt tcggtcacgt cgacgcaggt gtcttgcacg
     8161 tccgtccggc actggatatg tgcgatccgc aacaagagat tttgatgaag caaatctctg
     8221 atgacgtggt ggcgctgact gcgaaatacg gtggtttgtt gtggggcgag cacggcaaag
     8281 gttttcgcgc tgaatacagc ccggcgtttt tcggtgagga actttttgca gaactgcgca
     8341 aagtgaaagc ggcatttgac ccgcataacc gactcaaccc agggaagatt tgcccgccag
     8401 aaggtctcga tgcgccgatg atgaaagtgg acgcggtgaa gcgcggtaca ttcgatcggc
     8461 agatccccat tgcggtacgc cagcagtggc gcggtgcgat ggagtgtaac ggcaacggtt
     8521 tatgcttcaa ctttgatgcc cgtagtccga tgtgtccgtc gatgaagatc acccagaacc
     8581 ggattcattc accgaaaggg cgcgcaacgc tggtgcgtga atggctgcgc ttgttggcgg
     8641 atcgcggcgt tgacccactc aaactggaac aagaactgcc tgaatccggc gtcagtttgc
     8701 ggacgttaat tgcccgcacg cgcaatagct ggcatgcgaa taaaggcgaa tatgacttct
     8761 cacacgaagt caaagaggcg atgtcgggct gtctggcctg taaagcttgt tcgacccagt
     8821 gccccatcaa aattgatgtg ccggagtttc gctctcgttt tctgcagctc tatcacaccc
     8881 gttatttacg cccgcttcgc gaccacctcg ttgctacggt cgagagctac gcaccgctga
     8941 tggcacgcgc gccgaaaacc tttaacttct tcattaacca gccgctggtg cgcaaactct
     9001 cggaaaaaca tatcggcatg gttgatttgc cactgctgtc ggtcccctcg ctacaacaac
     9061 aaatggtggg gcatcgctcg gcaaacatga cgctggaaca gcttgaagct ctcaatgcag
     9121 agcagaaagc gcgcacggtg ttggtggtgc aggacccctt taccagctat tacgatgcgc
     9181 aagtggtggc ggattttgtc cgtctggttg aaaaattagg tttccagcct gtgttactgc
     9241 cattttcgcc aaatggcaaa gcccagcata tcaaaggctt tcttaatcgt tttgcgaaga
     9301 cggcgaaaaa gacggcggat ttcctcaacc gtatggctaa gcttgggatg ccaatggtgg
     9361 gcgtcgatcc ggcgctggta ctttgttatc gcgatgaata taaactggcg ctgggcgagg
     9421 aacgtggcga gtttaacgtc ttacttgcga atgaatggct ggcaagcgca cttgagtcac
     9481 atccagtggc tacagtcagc ggtgaatcat ggtatttctt tggtcactgt accgaggtta
     9541 ccgctttgcc gggcgcgcca gcacaatggg ccgcgatatt tgcccgtttt ggcgcgaaac
     9601 tggaaaatgt cagcgtgggt tgctgcggca tggcagggac ttacggacat gaagcgaaaa
     9661 accatgaaaa ttcgctcggg atctatgagt tatcctggca tcaggctatg cagcgactgc
     9721 cgcgtaaccg ctgtctggcg accggatatt cctgccgtag ccaggtaaaa cgggttgaag
     9781 gcacgggggt acgccatcct gtgcaggctt tactggagat tattaaatga tatggaaacg
     9841 gaaaatcacc ctggaagcac tgaatgctat gggtgaagga aacatggtgg ggttcctgga
     9901 tattcgcttt gaacatattg gtgatgacac ccttgaagcg acaatgccag tagactcgcg
     9961 gacaaagcag cctttcgggt tgctgcatgg aggagcatcc gtggtactgg ccgaaagtat
    10021 cggttccgtt gccggttatt tatgtaccga aggtgagcaa aaagtggttg gtctggaaat
    10081 caatgctaat cacgtccgct cggcacgaga agggcgggtg cgcggcatat gtaaaccgtt
    10141 gcatctcggt tcgcgtcacc aggtctggca gattgaaatc ttcgatgaga aagggcgttt
    10201 gtgctgttcg tcacgattga cgaccgccat tttgtgacgt tgtcaccaga aaagtgtgac
    10261 gcatattgct tttggtcatt atctgcacag taaacggtgg tatactgtgc aggtcttgtc
    10321 taaaagcgag gatgctatgt ctactcaact tgatcccacc cagctggcca ttgaattttt
    10381 acgccgtgac cagagcaatc tttcacctgc ccaatatctc aaacgtctga aacaactaga
    10441 acttgaattt gctgacctgc ttactctctc ttctgctgaa ttaaaagaag aaatctactt
    10501 cgcctggcgt ctgggcgtgc attgatcctg acttaatgcc gcaagttcgc caaatcgacc
    10561 tggattaacc tccgtccagg ttcgatgacc ttcttaatac tgcttatcta ccgcctgata
    10621 ttgttcaaaa ataatactaa tcgcagtttg tgttaaaacg gcgggttagc tttatgagat
    10681 tattcttatc gccccttcaa gagctaagcc actgagagtg ccggagataa gcgccggatg
    10741 gggtagaaac ccttaagcct gtgtcgcaca gacttaaggg ttttcttatt tctgaacgct
    10801 gcactttttt acctatctct taaatagtca ttttcaatac aactttttct ttttcatcac
    10861 agccttaatg ctccttagat aataaccatt atctaacaat gagataccta attcttagcg
    10921 tgcctgttaa cccacacatc aggggctatg cttattaaaa gcaggcaaaa agaatgtttt
    10981 acggtaaagc ccctgcgttt gctgggttga actgataatc attatcacta acatgctgtt
    11041 atacgctgaa agcgatgaag tgaggtaaat agatggacat gcattcagga acctttaacc
    11101 cacaggattt cgcctggcaa ggcttaacgc tgacacccgc agcggcgata cacatccgcg
    11161 agctggtggc aaagcagcca ggtatggtcg gcgtgcgctt aggtgtgaag caaacgggct
    11221 gcgcgggctt tggctatgtg ctcgacagtg ttagcgagcc ggacaaagac gatctgctgt
    11281 ttgaacacga cggcgcgaag ctgtttgtcc cgctgcaatc gatgccgttt attgatggca
    11341 cggaagtcga tttcgttcgt gaaggactta atcagatatt caaatttcac aaccctaaag
    11401 cccagaatga atgtggctgt ggcgaaagct ttagggtata ggcggtacta tgtctcgtaa
    11461 tactgaagca actgacgatg tcaaaacctg gaccggcggc ccgctgaact ataaagaagg
    11521 attcttcacc cagttagcca ccgatgagct ggcaaagggg ataaacgaag aggtggtgcg
    11581 cgcaatttcg gcgaagcgta atgagccgga gtggatgctg gagtttcgtc taaacgccta
    11641 tcgcgcatgg ctggagatgg aagaaccgca ctggttgaaa gcgcactacg acaagctgaa
    11701 ttatcaggat tacagctact actcagcacc atcgtgcggt aattgtgacg acacttgcgc
    11761 gtctgaacct ggcgcggtgc agcaaactgg cgcgaacgcc tttttaagta aagaggtgga
    11821 ggcggcgttt gagcagttgg gcgttcccgt gcgggaaggc aaagaggtgg cggtggatgc
    11881 cattttcgac tctgtttcgg ttgccaccac ttatcgcgaa aaactggcgg agcagggaat
    11941 tattttctgt tcctttggtg aggcgatcca cgatcacccg gaactggtgc gtaaatatct
    12001 cggcaccgtg gtgccgggga atgacaactt ctttgcagca cttaatgcgg cggtagcctc
    12061 tgacggtacg tttatttatg tgcctaaagg cgtgcgctgc ccgatggaac tttccaccta
    12121 ttttcgcatt aacgcggaaa aaaccgggca gtttgagcgc acaattctgg tggccgacga
    12181 agacagctac gtcagctaca ttgaaggctg ttccgctccg gtgcgtgaca gctatcagtt
    12241 gcacgcggca gtggtggaag tcatcatcca taaaaacgcc gaggtgaaat attccacggt
    12301 acaaaactgg ttccctggcg ataacaacac cggcggtatt ctcaacttcg tcaccaagcg
    12361 tgctttgtgc gaaggcgaaa acagcaaaat gtcatggacg caatcagaaa ccgggtcagc
    12421 gattacgtgg aaatatccca gctgcatttt gcgcggcgat aactccattg gtgagtttta
    12481 ctcagtggcg ctgaccagcg gtcatcagca agcggatacc ggcaccaaga tgatccacat
    12541 cggtaaaaac accaaatcga ccattatctc gaaagggatc tctgccggac atagtcagaa
    12601 cagttatcgc ggcttagtga aaatcatgcc gacggcaacc aatgcgcgca atttcactca
    12661 gtgcgactca atgctgattg gcgctaattg tggggcgcat accttcccgt atgtcgaatg
    12721 tcgcaataac agcgcacaac tggagcacga ggcaacgaca tcacgtattg gtgaagatca
    12781 actgttttac tgcctgcaac gcgggatcag cgaagaagac gccatctcga tgattgttaa
    12841 cggtttctgc aaagacgtgt tctcagaact gccgctggaa tttgccgttg aagcacaaaa
    12901 actcctcgcc atcagtcttg aacacagcgt cggataagga ataaacatgt taagtattaa
    12961 agatttacac gtcagcgtgg aagataaagc tatcctgcgt ggattaagcc tcgacgttcg
    13021 tcccggcgaa gttcacgcca ttatggggcc aaacggttcg ggcaaaagta ccttatcggc
    13081 aacgcttgcc gggcgcgaag attatgaagt gacgggcggc acggttgagt tcaaaggcaa
    13141 agatttgctt gcactgtcgc cggaagatcg tgcaggcgaa ggcatcttta tggccttcca
    13201 gtatccggtg gagattccag gcgtcagtaa ccagtttttc ctgcaaacgg cacttaatgc
    13261 agtgcgcagc tatcgcggcc aggaaacgct cgaccgcttt gattttcagg atttgatgga
    13321 agagaaaatc gctctcctga agatgccgga agatttatta acccgttcgg taaacgttgg
    13381 tttttccggc ggcgagaaaa agcgcaacga tattttgcaa atggcggtgc tggaaccgga
    13441 gttatgcatt cttgatgagt cggactccgg gctggatatt gacgcattaa aagtggtcgc
    13501 cgatggcgtg aactcgctgc gtgatggcaa gcgctcattc atcattgtta cgcactacca
    13561 acgcattctc gactacatca agcctgatta cgttcatgtg ctatatcagg gacgaattgt
    13621 gaaatccggc gatttcacgt tggtcaaaca actggaggag cagggttatg gctggcttac
    13681 cgaacagcag taatgcgctg caacagtggc atcacttgtt tgaagctgaa gggacaaaac
    13741 gctcaccgca agcacagcag catttacaac aattgctgcg taccggactg ccgacacgta
    13801 aacatgaaaa ctggaaatat acgccgctgg aagggctgac caatagtcag tttgtcagca
    13861 ttgcgggaga tatatcccca cagcagcgtg atgccttagc gttaacgtta gacgccgtgc
    13921 ggctggtgtt tgtcgatgga cgttacgtgc ccgcgctgag cgatgcaatt gaaggcagcg
    13981 gatatgaagt gagcattaac gacgaccgtc aggggttacc cgacgctatt caggcggaag
    14041 tgtttctgca tttgacggaa agcctggcac aaagcgtgac gcatatcgcc gtgaagcgcg
    14101 gtcaacggcc ggcaaagcca ttgctgttaa tgcatatcac ccagggcgtg gcaggtgaag
    14161 aggtgaacac tgcccattac cggcatcatc tggatctggc ggaaggtgcc gaagcaacgg
    14221 tgatcgaaca ttttgtcagc cttaatgatg ctcgtcattt taccggggca cggttcacta
    14281 tcaatgtcgc agcgaacgcc cacttgcagc atatcaagct ggcgtttgaa aacccggtca
    14341 gtcaccactt tgcccataac gatttgttgc tggctgacga tgccaccgca tttagccata
    14401 gtttcctgct gggtggcgca gtgttacgac acaacaccag tacgcaactc aatggcgaaa
    14461 acagcacgct gcggatcaat agcctggcga tgccggtgaa aaacgaggtg tgtgataccc
    14521 gtacctggct ggaacacaat aaaggttttt gtaacagccg acagttgcac aaaactatcg
    14581 tcagcgacaa aggccgcgcg gtatttaacg gtttgatcaa cgtcgcgcag cacgccatca
    14641 aaacggatgg tcagatgacc aacaataatc tgctgatggg caaactggcg gaagtggata
    14701 caaaaccgca gctggaaatc tatgcagatg atgtgaaatg cagccacggc gcgacggtgg
    14761 ggcgtattga tgatgaacag atgttctatc tgcgctcgcg cgggatcaat cagcaggatg
    14821 cccagcagat gatcatttac gcctttgctg ccgaactgac ggaagcactg cgtgatgagg
    14881 ggcttaaaca gcaggtgctg gcccgaatcg gtcaacggct gccaggaggt gcaagatgac
    14941 tttttccgtc gacaaagtgc gggccgactt tccggtgctt tcgcgtgagg taaacggttt
    15001 gccgctggct tatctcgaca gcgccgccag tgcacagaaa ccgagccagg tgattgacgc
    15061 cgaggccgag ttttatcgtc atggctacgc ggcggtgcat cgcggtattc ataccttaag
    15121 cgcccaggcg accgagaaaa tggagaacgt gcgcaagcgg gcatcgctgt ttattaatgc
    15181 ccgttcggcg gaagagctgg tgtttgtccg cggcacgacg gaagggatca atctggtcgc
    15241 caatagctgg ggcaacagca atgtgcgggc gggcgataac atcatcatca gtcagatgga
    15301 gcaccacgct aacattgtcc cctggcagat gctttgcgca cgcgttggcg cagagctgcg
    15361 tgtgatccct ctcaaccccg acggtacgct gcaactggag acgctgccta cgctgtttga
    15421 tgagaaaact cgcctgctgg caattactca tgtctccaac gtgcttggca cagaaaatcc
    15481 actggcggaa atgatcacgc ttgcgcacca gcatggcgca aaagtgctgg tggatggcgc
    15541 tcaggcggtg atgcatcatc cggtggatgt tcaggcgctg gattgcgact tttacgtgtt
    15601 ctccgggcat aaactgtatg gccccaccgg aattggcatt ctttatgtga aagaagcctt
    15661 gttgcaggag atgccgccgt gggaaggggg cggttctatg atcgccaccg tcagcctgag
    15721 tgaaggcact acctggacca aagcaccatg gcggtttgaa gccggtacac ccaataccgg
    15781 gggcatcatt ggtcttggcg cggcgctgga gtatgtttcg gcgctggggc ttaatagcat
    15841 agccgagtat gaacagaatc tgatgcatta tgcgctatca cagctggaat ctgtaccgga
    15901 tctcactctc tatggccaac aaaacaggct tggcgttatt gcttttaatc tcggtaaaca
    15961 ccacgcctat gatgttggca gttttctcga taattacggc attgctgtgc gtaccggaca
    16021 tcactgcgct atgccattaa tggcctatta caacgtccct gcgatgtgtc gggcgtcgct
    16081 ggccatgtat aacacccatg aagaagtgga gcgtctggtg accggcctgc aacgtattca
    16141 ccgtctgctg ggataacagg gaggcactat ggctttattg ccggataaag aaaaattgct
    16201 gcgtaatttt ttacgctgcg ccaactggga agagaaatat ctctacatta ttgagctggg
    16261 ccagcgtctg ccagaattac gcgccgaaga cggaagccca caaaatagca ttcagggctg
    16321 ccagagtcag gtgtggattg tcatgcgcca gaatgcgcag ggaattattg aattgcaggg
    16381 cgacagcgat gcggcgattg tgaaagggct tattgcggtc gtctttattc tctacgatca
    16441 gatgacgccg caggatattg tcaatttcga tgtgcgtccg tggtttgaaa aaatggcgct
    16501 cacccaacat ctcaccccat ctcgttcaca aggtctggaa gcgatgattc gcgcaattcg
    16561 cgccaaagcc gctgcactta gctaaactaa agggacagct ttcatccggt tggctcacat
    16621 tgccggatgg tcaatgttta cactgaccgg cacaggagtg ttttggtttc aggtgaacat
    16681 aagcaccatc cgacgtttca gcgtgagtct ccggcaaata caggaggttt acaatgaaac
    16741 gcgcgtcttt gcttacactc acgcttatcg gcgcttttag cgccatccag gctgcctggg
    16801 cggttgatta tccgctacca ccaaccggaa gccgactggt tgggcaaaat caaacgtata
    16861 cggtgcaaga aggggataaa aaccttcagg ctattgcccg acgttttgat actgcagcaa
    16921 tgttgatcct tgaagccaat aacactatcg ccccggtgcc aaaacctggt acgacgataa
    16981 ctattccttc acaactgtta ttacctgatg caccgcgtca ggggattatc gttaaccttg
    17041 cagagctgcg cctttattat tatccgccgg gagaaaatat tgtgcaggtt tatccaatag
    17101 gtattggatt gcaggggctg gaaacgccgg tgatggaaac gcgtgttggg cagaaaatcc
    17161 ctaacccaac ctggacgcct acggcaggca ttcgtcagcg ttcgctggag cgtggcatta
    17221 aattaccgcc agtcgttcct gccggaccaa ataacccgct aggacgttac gcactgcgcc
    17281 tcgcgcatgg taatggcgaa tacctcattc atggcaccag tgcgccggac agcgttggtt
    17341 tgcgcgtcag ttcagggtgt attcgcatga atgcaccgga tattaaagcc ttgttctcca
    17401 gcgtgcggac gggaacgccg gtgaaagtga tcaacgaacc ggtgaaatat tccgtggaac
    17461 ctaacgggat gcgttatgtt gaagtacatc gaccactatc agcagaagaa cagcaaaacg
    17521 ttcagacaat gccatacaca ctgccagcag gctttacgca atttaaagac aataaggctg
    17581 tagatcagaa gttagtcgat aaagcgttgt atcgtcgggc agggtatccg gtttcggtga
    17641 gcagtggagc aactcccgca gccagcaatg cgccttcagt agagtcagcg cagaatggtg
    17701 aaccagagca agggaatatg ttacgcgcga cgcagtagtg gtaaatagca gacaaaaaaa
    17761 tggcgcacaa tgtgcgccat ttttcacttc acaggtacta ttacttgcgg tatttagtag
    17821 ccatgttgtc cagacgctgg ttagcacgag ctgcgtcatc tttagcagcc tgaacgtcgg
    17881 aacgcattgc gttcacgtcg ttgctcagct ggtcaacttt agcgttcaga gtctgaacgt
    17941 cagaagacag ctgatcgatt ttagcgttgc tggagcaacc tgccagcaga gtagaaccca
    18001 ggattaccgc gcccagtacc agtttagtag ctttcattat taataccctc tagattgagt
    18061 taatctccat gtagcgttac aagtattaca caaagttttt tatgttgaga atattttttt
    18121 gatgggaagg cacttatttt tgatcgttcg ctcaaagaag catcgaacgc tgggttttag
    18181 ataaaaaaat tgagagaaaa caggatgctt ccatc
//
LOCUS       DAZPCS010000071        18045 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZPCS010000071 DAZPCS010000000
VERSION     DAZPCS010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18045
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.071"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            8..1088
                     /locus_tag="ACHUQ7_002325"
                     /pseudo
     CDS             8..1088
                     /locus_tag="ACHUQ7_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
     gene            complement(1234..2271)
                     /gene="iap"
                     /locus_tag="ACHUQ7_002326"
     CDS             complement(1234..2271)
                     /gene="iap"
                     /locus_tag="ACHUQ7_002326"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFR1135710.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGGCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            2523..3431
                     /gene="cysD"
                     /locus_tag="ACHUQ7_002327"
     CDS             2523..3431
                     /gene="cysD"
                     /locus_tag="ACHUQ7_002327"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFR1135711.1"
                     /translation="MAQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            3433..4860
                     /gene="cysN"
                     /locus_tag="ACHUQ7_002328"
     CDS             3433..4860
                     /gene="cysN"
                     /locus_tag="ACHUQ7_002328"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFR1135712.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            4860..5465
                     /gene="cysC"
                     /locus_tag="ACHUQ7_002329"
     CDS             4860..5465
                     /gene="cysC"
                     /locus_tag="ACHUQ7_002329"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFR1135713.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLSVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            5515..5838
                     /locus_tag="ACHUQ7_002330"
     CDS             5515..5838
                     /locus_tag="ACHUQ7_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFR1135714.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFLYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            6032..6343
                     /gene="ftsB"
                     /locus_tag="ACHUQ7_002331"
     CDS             6032..6343
                     /gene="ftsB"
                     /locus_tag="ACHUQ7_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFR1135715.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            6362..7072
                     /gene="ispD"
                     /locus_tag="ACHUQ7_002332"
     CDS             6362..7072
                     /gene="ispD"
                     /locus_tag="ACHUQ7_002332"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFR1135716.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            7072..7551
                     /gene="ispF"
                     /locus_tag="ACHUQ7_002333"
     CDS             7072..7551
                     /gene="ispF"
                     /locus_tag="ACHUQ7_002333"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFR1135717.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            7548..8597
                     /gene="truD"
                     /locus_tag="ACHUQ7_002334"
     CDS             7548..8597
                     /gene="truD"
                     /locus_tag="ACHUQ7_002334"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFR1135718.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            8578..9339
                     /gene="surE"
                     /locus_tag="ACHUQ7_002335"
     CDS             8578..9339
                     /gene="surE"
                     /locus_tag="ACHUQ7_002335"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFR1135719.1"
                     /translation="MRILLSNYDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     QDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            9333..9959
                     /gene="pcm"
                     /locus_tag="ACHUQ7_002336"
     CDS             9333..9959
                     /gene="pcm"
                     /locus_tag="ACHUQ7_002336"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFR1135720.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            10144..11238
                     /gene="nlpD"
                     /locus_tag="ACHUQ7_002337"
     CDS             10144..11238
                     /gene="nlpD"
                     /locus_tag="ACHUQ7_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFR1135721.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPTATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            11301..12293
                     /gene="rpoS"
                     /locus_tag="ACHUQ7_002338"
     CDS             11301..12293
                     /gene="rpoS"
                     /locus_tag="ACHUQ7_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFR1135722.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRK"
     gene            complement(12413..12820)
                     /locus_tag="ACHUQ7_002339"
     CDS             complement(12413..12820)
                     /locus_tag="ACHUQ7_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFR1135723.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            12967..13560
                     /locus_tag="ACHUQ7_002340"
     CDS             12967..13560
                     /locus_tag="ACHUQ7_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit B"
                     /protein_id="HFR1135724.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            13560..14507
                     /locus_tag="ACHUQ7_002341"
                     /pseudo
     CDS             13560..14507
                     /locus_tag="ACHUQ7_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
     gene            complement(14780..15343)
                     /locus_tag="ACHUQ7_002342"
     CDS             complement(14780..15343)
                     /locus_tag="ACHUQ7_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1135725.1"
                     /translation="MRKAPQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGT
                     LKNYYNRRDYAPIPRPYQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDA
                     QILMFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLP
                     DEKVREIYALSEAKGDHLNLPQKAASA"
     gene            15508..15759
                     /locus_tag="ACHUQ7_002343"
     CDS             15508..15759
                     /locus_tag="ACHUQ7_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR1135726.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            15761..17857
                     /locus_tag="ACHUQ7_002344"
     CDS             15761..17857
                     /locus_tag="ACHUQ7_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HFR1135727.1"
                     /translation="MQSYVTVNDLLGVPGMPATTKGIRQALQRFSRDLGDVSRRREGT
                     KAIEYHIDCLPEITRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGS
                     PQLMEERLNALTENQRWVSEARAALVVEVLKLESAGNPGRLKAINFLVEKARKGELPE
                     RLQQAAVNANAKRGANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQVNGRPMSEAYEDFVAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVV
                     GILPRLGIKHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRESLRVLNRD
                     LRSAFNALQQDKPLNDRQKAAMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRH
                     YSPTEFRKKRQAEEDTEIEWLSDLELRDMFRPMVERPVRRCEIQWLNNIYYAPELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFAVSAEYHKQQQRIKGMRK
                     RAEEKIRDAEDEGIQILEHKQAEPWLSNVYRPVGNVVAVQQPEYEEEHDEEFERDFRL
                     GMQKLFAMQEEDDPLA"
BASE COUNT         4456 a         4403 c         4838 g         4348 t
ORIGIN      
        1 ttaaaatatg cgtaaatatc ctttaagttt actgaaggat aaaaatattg tgactttctt
       61 tgatttctgg ggaaaaaacc gacgtggcga gaaagagggt atcgacgact atcaccttct
      121 ttgctggcat ttgctggatg tggccgcaat gggctattta atggttaaaa gaaattgctt
      181 cgggctggct gattattttc gtcaattagg gatttctgac aaggaacagg cagctcgatt
      241 tttcgcctgg ttgctgtgct ggcacgatat tggaaaattt gcccgctctt ttcaacaact
      301 ttacctgccc cctgaattca agattcagga aggtgccaga aaaaatctct cattcaacgc
      361 tgggctactg gttgtggaat cattatttaa gtgaatgtca ggagttactt ccttcatctt
      421 cgctctctcc gcgcaaactt aggcgagtga tagaaatgtg gatgcccgtg acgacagggc
      481 atcatggtcg gccccctgat cgtatggatg aattggataa ttttctgcct gaagacaaag
      541 ctgcagcacg agattttctc cttgaaatca agccactgtt tccgcgaata gagatcccca
      601 cattctggga tgatgacgag ggcgttgaac ttataaaaca actttcctgg tatatctctg
      661 caaccgtcgt acttgcagac tggacagggt cgtcaacgcg attttttcca cgcgtcgcac
      721 aggcaatgga tattgaagat tactggcaaa aggctttagt tcaggctcaa aacgcattaa
      781 ccgtctttcc gccacaagca gaaaccgcgc ctttcaccgg aattaatacg ctgtttcctt
      841 ttattgagaa tacgacaccg ttacagcaaa aggtgctgga tctggatatc aatcagccag
      901 gaccacagtt gtttattctg gaagatgtga ccggcgcagg taaaacagaa gcggcgctta
      961 tcctggcgca caggttgatg gctgcgggta acgctcaggg gctgtttttt ggcctgccaa
     1021 caatggcaac ggccaatgcc atgtacgatc gactggtcaa aacctggctt gctttctatt
     1081 cgccagaggt gttccccgct ccagcggaga taaaccggcg caatacggtg cggttttccc
     1141 ggaagtaaac cggacgcact ggatgcgatg atgggtatca cttagaattc cccgcccctg
     1201 cggtagaact cccagctccc attttcaaac ccatcaagac gccttcgcca gctccttcac
     1261 caacggtagc attatccgca taacgtcacg gcagcgacgt tctattcttc caggaagtgc
     1321 cttatcaata tgttgttgat tatccagtct tacgtcatgc cagctatttc ccgccgggaa
     1381 ggcaggtgtt tttgcgcgtt gctgataacc atccttattt ccaagattcc agttagtcgc
     1441 ttccaccgaa agtacagcaa tgcccgcttt gtcgaatatt tctgcgtcat tacaaccccc
     1501 agtgcctttc ggataatttt tattcaaacc cggattggtc gttgcggcta ttccgtgact
     1561 gcgtgcaatt gccagcgccc tgtcgcgcgt taatttcctt actgcttcag gggtttttac
     1621 accgctgttg aaatacaatt tatcgccaac aattaagtta tcgagattaa tcaccagcag
     1681 cgtatttttc ttttcggtgt cactcatccg cttgagtaaa ttctcagcgc ctaattttcc
     1741 ttcctcttcg ccgctggtcg ccacaaatcg aataccatac tcggtaggcg tatttttcag
     1801 gcgttctgcc agttccagca tgacacctaa acctgcggcg ttatcatcta ttccttgtaa
     1861 cgtcagtccg ccgagattgg catctgcgtc agcatcgctc agcggggcgt aagtatccag
     1921 atgcgccata atgatgatct gctgcggcgc tttgccttca tgagcggcaa tcaccgtact
     1981 tcctgtcacg ttatgccagt tcttgcgatt atcgcgggcg gtataaatat accgactatt
     2041 aaatgtccga atatcactgc gataacccat ttgctgaaac tgttggcgaa tataatcggc
     2101 agataacatt tctgctggag ttccggtcat gcgtcccgga aagaaagtag caatatgtcg
     2161 tgcctgggta ttagcaaaat cgccaggttt aggtgacgag gcgtgtacgg ggagaataaa
     2221 gcatacgccg agcgccaggg cagcggtacg gtggcgcaat gcggaaaaca tagtgagtcc
     2281 ttaaatacca tgcaaatttt tttaccgcca tagtatgaaa ctgccgctgc gctaaaacaa
     2341 tttcaaatct tcctaaacgc ccgaaatccg gtgccttaag cactttttga tattagcttt
     2401 gccaaatcgt tattccgtta aggaactact cattctaatt ggtaatttca ttcgttctct
     2461 tacgctccct atagtcgaaa catctgatgg caagaaaata gcggtattgc aaaggaacgg
     2521 ttatggctca aaaacgactt actcacctgc ggcaactgga ggcggaaagc atccacatta
     2581 ttcgcgaggt ggcggcagaa ttctcaaatc cggtgatgct ctactctatc ggtaaagatt
     2641 ccagcgtcat gctgcatctg gcgcgcaagg cgttttatcc aggtacgctg cctttcccgt
     2701 tgctgcatgt cgataccggc tggaaattcc gcgagatgta tgagttccgc gatcgtacgg
     2761 cgaaagccta cggctgcgag ctgctggtgc ataaaaaccc ggaaggcgtg gcgatgggga
     2821 ttaatccatt cgtgcacggc agcgcgaaac ataccgatat tatgaaaact gaaggcctga
     2881 aacaggcgct gaacaaatac ggttttgatg ccgccttcgg tggtgcgcgc cgtgacgaag
     2941 agaaatcccg cgctaaagag cgaatctact ctttccgtga ccgcttccat cgctgggatc
     3001 cgaaaaatca gcgcccggag ctgtggcaca actacaacgg gcaaattaac aaaggcgaaa
     3061 gcatccgcgt cttcccgctc tctaactgga ccgagcagga tatctggcaa tacatctggc
     3121 tggaaaatat cgacattgtg ccgctgtatc tcgctgcgga acgtccggtt ctggaacgcg
     3181 acggtatgtt gatgatgatt gatgacaacc gtatcgacct gcaaccgggc gaagtgatca
     3241 aaaaacggat ggtgcgtttc cgtacgctgg gctgctggcc gctgaccggt gcggtggagt
     3301 caaatgcaca aacactgccg gaaatcatcg aagagatgct ggtttccacc accagtgaac
     3361 gtcagggtcg cgtgattgac cgcgaccagg cgggatccat ggagctgaaa aaacgtcagg
     3421 ggtattttta agatgaacac cgtacttgca caacaaatcg ccaatgaagg cggcgtcgaa
     3481 gcctggatga ttgcgcaaca acataaaagc ctgctgcgtt ttctgacctg tggtagcgtc
     3541 gatgacggca aaagtaccct gattggtcgt ctgctgcacg atacccgcca aatctacgaa
     3601 gatcagctct catcgctgca taacgacagt aagcgtcacg gaacccaggg cgaaaagctg
     3661 gatctggctc tgctggtgga cggcctgcaa gctgagcgcg aacagggcat cactattgat
     3721 gtggcctacc gctatttctc taccgagaag cgtaaattta ttatcgccga caccccaggg
     3781 cacgagcagt acactcgcaa tatggcgact ggcgcatcga catgtgaact ggcgatctta
     3841 ctgattgatg cccgtaaagg cgtgctcgat caaacccgtc gtcacagttt tatctccaca
     3901 ctgttgggga tcaaacatct ggtcgtggcg atcaacaaaa tggatctggt ggattacagt
     3961 gaagagacgt tcacccgtat tcgtgaagat tatctgacct ttgccgggca gctgccgggt
     4021 aatctggata tccgctttgt gccactctcc gcactggaag gtgacaacgt ggcttcgcaa
     4081 agtgaaagta tgccgtggta cagcggtctg acactgctcg aagtgctgga aaccgtggag
     4141 atccagcgag tggtggatgc ccagccaatg cgcttcccgg tgcagtacgt taaccgccca
     4201 aatctcgatt ttcgtggcta cgccggaatg ctggcatccg gtcgcgtgga agtcgggcaa
     4261 cgtgtaaaag tgctgccctc tggtgtggaa tcaaacgtcg cgcggatcgt gacttttgat
     4321 ggtgatcgcg aagaagtctt tgccggagaa gcgatcaccc tggtgctgac ggatgagatc
     4381 gatatcagcc gtggtgatct gctgctggcg gcagacgaag cgttaccggc ggtgcagagt
     4441 gcgtcggtgg atgtggtatg gatggcggaa cagccgcttt ccccgggcca gagttatgac
     4501 atcaaaattg ccggtaagaa gacgcgtgct cgtgttgatg gcattcgtta tcaggttgat
     4561 attaataacc ttacccaacg cgaagttgaa aacctgccgc tgaacggcat cggcctggtg
     4621 gatctcactt ttgacgagcc actggtgtta gatcgctatc agcaaaatcc ggttaccggt
     4681 gggctgattt ttatcgatcg cctgagcaat gtgaccgtgg gtgccggtat ggtgcacgag
     4741 ccagttagcc aggcaacagc tgcgtcatct gaattcagtg cattcgaact ggaattgaat
     4801 gccctggttc gtcgccactt tccgcactgg ggcgcgcgcg atttgctggg ggataaataa
     4861 tggcgctgca tgacgaaaac gtcgtctggc atagtcatcc ggtcactgtg caacaacgcg
     4921 aactacacca cggtcatcgt ggtgtagtgc tgtggtttac cggcctctcc gggtccggta
     4981 aatcaacggt cgccggggcg ctggaggagg cgttacataa actcagcgtc agtacgtatc
     5041 tgctggatgg cgacaatgtt cgccacggat tatgcagcga tctcggtttt agcgatgccg
     5101 atcgtaaaga gaatatccgt cgcgtcggtg aagtggcgaa tttgatggtt gaagccggac
     5161 tggtggtgct gaccgcattt atctcgccac accgcgccga acgccagatg gttcgcgaac
     5221 gcgtaggaga agggcgcttt attgaagtgt ttgtcgatac gccgctggcg atttgcgaag
     5281 cccgcgatcc aaaaggctta tataagaaag cgcgtgccgg tgaactgcgc aactttacgg
     5341 gaatagattc cgtttacgaa gcgcctgaat cggcagaaat tcatctcaat ggtgaacaat
     5401 tagtaacaaa tttggtacag caattattag atctgttgag acagaacgat attatcagat
     5461 cctgagacac caccgggttt ccatgcccgg ttcgtcaaag taacaggatc aaatatgcgt
     5521 aatagccata acattacact aacaaataac gacagcctta ctgaggatga agaaactaca
     5581 tggtcactgc ctggtgccgt ggtcggtttt atctcctggt tatttgcgct ggcgatgccg
     5641 atgttgattt atggctctaa cacgctgttc ttctttctct acacctggcc tttctttctg
     5701 gcactgatgc ctgtcgcggt agtggtgggg attgcgctgc attcattgat ggacggaaag
     5761 ctacgctaca gtattgtttt caccctggtg acagtaggca ttatgtttgg cgcactgttt
     5821 atgtggctac tgggctaatt tgtactttct tgtctcctct gttcataatt caaaccgtaa
     5881 ctaataatga gattatgttc tgcacgccct gggtatacgt aacaatgggc aaatgtggta
     5941 catttgcccg cgttgtcgcg gtatccccaa cagaggatgt agagtcgtct tcggatgcat
     6001 gggatgatga tgcagttttt cagggggcag gatgggtaaa ctaacgctgc tgttgctggc
     6061 tattctggtc tggctacagt attcgctgtg gttcggtaag aacggtatac atgactatac
     6121 ccgcgtcaat gatgatgtgg cggcactgca agctacaaac gcgaaactta aagcgcgaaa
     6181 cgatcaactt tttgccgaaa ttgacgatct caatggcggc caggaggcgc tcgaagagcg
     6241 tgcgcgtaat gaactcagca tgaccaggcc gggcgaaact ttttatcgtc tggtgcctga
     6301 cgcgtcgaag cgcgcacagt ctgcggggca aaacaatcga taaatcagcc cgggaattaa
     6361 catggcaacc actcatttgg atgtttgcgc cgtggttccg gcggccggat ttggccgtcg
     6421 aatgcaaacg gaatgtccta agcaatatct ctcaatcggt aatcaaacca ttcttgaaca
     6481 ctcggtgcat gcgctgctgg cgcatccccg ggtgacacgt gtcgtcattg ccataagtcc
     6541 tggcgatagc cgttttgcac aacttcctct ggcgaatcat ccgcaaatca ccgttgtaga
     6601 tggcggtgat gagcgtgccg attccgtgct ggcaggtctg aaagccgctg gcgatgtgca
     6661 gtgggtattg gtgcatgacg ccgctcgtcc ttgtttgcat caggatgacc ttgcgcgatt
     6721 gttggcgttg agcgaaacca gccgcacggg agggatcctc gccgcaccag tgcgcgatac
     6781 tatgaaacgt gccgaaccgg gcaaaaatgc cattgctcat accgttgatc gcaacggctt
     6841 atggcacgcg ctgacgccgc aatttttccc tcgtgagctg ttacatgact gtctgacgcg
     6901 cgctctaaat gaaggcgcta ccattaccga cgaagcctcg gcgctggaat attgcggatt
     6961 tcatccgcag ttggtcgaag gccgcgcgga taacattaaa gtcacgcgcc cggaagattt
     7021 ggcactggcc gagttttacc tcacccgaac catccatcag gagaatacat aatgcgaatt
     7081 ggacacggtt ttgacgtaca cgcctttggc ggtgaaggcc caattatcat tggtggcgta
     7141 cgcattcctt acgaaaaagg attgctggcg cattctgatg gcgacgtggc gcttcatgcg
     7201 ttgaccgatg cattgcttgg cgcggcggcg ctgggggata tcggcaaact gttcccggat
     7261 accgatccgg catttaaagg tgccgacagc cgcgagctgc tacgcgaagc ctggcgtcgt
     7321 attcaggcga agggttatac ccttggcaac gtcgatgtca ctatcatcgc tcaggcaccg
     7381 aagatgttgc cgcacattcc acaaatgcgc gtgtttattg ccgaagatct cggctgccat
     7441 atggatgatg ttaacgtgaa agccactact acggaaaaac ttggatttac cggacgtggg
     7501 gaagggattg cctgtgaagc ggtggcgcta ctcattaagg caacaaaatg attgagtttg
     7561 ataatctcac ttacctccac ggtaaaccgc aaggcaccgg gctgctgaaa gccaatccgg
     7621 aagactttgt ggtggtggaa gatttgggct ttgagcctga tggtgaaggt gagcatattc
     7681 tggttagaat cctcaaaaac ggctgcaata cccgttttgt ggcggatgca ctggcgaaat
     7741 tcctgaaaat tcattcccgt gaagtcagct tcgctgggca aaaagacaaa catgctgtaa
     7801 cggaacagtg gttatgtgct cgcgtgccgg gcaaggaaat gcccgatctg agcgcctttc
     7861 aactggaagg ctgccaggtg ctggagtatg cgcggcacaa gcgcaagctg cgtttagggg
     7921 cgctgaaagg taacgccttt accctggttc tgcgcgaagt gagcaatcgc gatgacgttg
     7981 aacaacgtct gatcgatatt tgcgtaaaag gtgtaccgaa ctacttcggt gcccaacgtt
     8041 ttgggattgg cggtagcaac ttgcagggcg cgctgcgctg ggcgcaaacc aatactccgg
     8101 tgcgcgatcg caataaacgg agtttttggt tgtcggcagc ccgcagtgcg ttgtttaatc
     8161 agattgttgc tgagcgcctc aaaaaagcag acgttaatca agttgttgac ggcgatgcgc
     8221 tacaattagc cggacgtggt agctggtttg tagcaaccac cgaagaactg gcggaattac
     8281 agcgtcgcgt caacgataaa gagctgatga taaccgccgc attgccgggc agtggcgaat
     8341 ggggaactca gcgtgaagcg ctggcattcg aacaagcagc tgtcgccgca gaaactgaat
     8401 tacaagcttt actggtgcgc gaaaaagttg aagccgcgcg cagagcgatg ctgctgtatc
     8461 cgcaacaatt aagctggaac tggtgggatg acgtcaccgt agagatccgt ttctggcttc
     8521 cggcgggtag ttttgcaacc agcgttgtca gggaacttat caacacaaca ggtgattatg
     8581 cgcatattgc tgagtaatta tgacggggta catgcacccg gtatacaaac gctggcgaaa
     8641 gccttgcgtg agtttgctga cgttcaggtg gtcgcccccg atcgtaaccg cagcggcgct
     8701 tcaaattctc tgacactgga atcctccctg cgcacgttta cctttgaaaa tggtgatatt
     8761 gctgtgcaaa tgggaacccc gaccgattgt gtctatcttg gcgtgaatgc tctgatgcgt
     8821 ccgcgcccgg acattgttgt gtccggaatt aacgccgggc cgaatctggg ggatgatgtt
     8881 atttattccg gtacggtagc cgccgcgatg gaaggccgtc atttaggttt tccggcgctg
     8941 gccgtctcgc ttgacgggca taaacattac gacactgccg cggcggtaac ctgttcaatt
     9001 ttgcgcgcac tgtgtaaaga gccgctgcgc accggacgta ttcttaatat taacgttcag
     9061 gatttaccct tggatcaaat caaaggtatt cgcgtgacgc gctgcggtac acgacatccg
     9121 gcagatcagg tgatcccgca gcaagatccg cgcggcaata cgctgtactg gattggcccg
     9181 ccgggcggta aatgtgatgc cggtccgggg accgattttg ctgcggtaga tgagggctat
     9241 gtctccatca cgccgctgca tgtggattta actgcgcata gcgcgcaaga tgtggtttca
     9301 gactggttaa acagcgtggg agttggcacg caatggtaag cagacgcgta caagcacttc
     9361 tggatcaatt acgtgcgcaa ggtattcagg atgagcaggt gctgaatgca cttgccgccg
     9421 tgccgcgtga aaaattcgtt gatgaagcgt ttgaacaaaa agcctgggac aatatcgcct
     9481 tgccgatagg tcaggggcag acaatttcgc agccgtatat ggtggcgcga atgaccgaat
     9541 tactcgagct gacgccgcag tcgcgggtgc tggaaattgg cactggttcg ggatatcaaa
     9601 cggcaatcct ggcgcatctt gtccagcatg tttgctcggt tgaacggatt aaaggcttgc
     9661 agtggcaggc acgtcgccgc ctgaaaaatc ttgatttaca taatgtttca acccgtcatg
     9721 gtgatggatg gcaaggttgg caggcacgtg cgccgtttga cgctatcatt gttacggcgg
     9781 caccgccgga aattccaact gcgctaatga cgcagctgga cgaaggcggg attctcgtct
     9841 tacccgtagg ggaggagcac cagtatttga aacgggtgcg tcgtcgggga ggcgaattta
     9901 ttatcgatac cgtggaggcc gtgcgctttg tccctttagt gaagggtgag ctggcttaaa
     9961 acgtgaggaa atacctggat ttttcctggt tattttgccg taggtcagcg tatcgtgaac
    10021 atcttttcca gtgttcagta gggtgccttg cacggtaatt atgtcactgg ttattaacca
    10081 atttttcctg ggggataaat gagcgcggga agcccaaaat tcaccgttcg ccgcattgcg
    10141 gctttgtcac tggtttcgct atggctggca ggctgttctg acacttcaaa tccaccggca
    10201 ccggtcagct ccgttaatgg caatgcgcct gcaaatacta attctggtat gttgattacg
    10261 ccgccgccga aaatggggac gacgtctaca gcgcagcaac cgcaaattca gccggtacag
    10321 cagccacaaa ttcaggctac tcaacaaccg caaatccagc cagtgcagcc agtagctcag
    10381 cagccggtac agatggaaaa cggacgcatc gtctataacc gtcagtatgg gaacattccg
    10441 aaaggcagtt atagcggcag tacctatacc gtgaaaaaag gcgacacact tttctatatc
    10501 gcctggatta ctggcaacga tttccgtgac cttgctcagc gcaacaatat tcaggcacca
    10561 tacgcgctga acgttggtca gaccttgcag gtgggtaatg cttccggtac gccaatcact
    10621 ggcggaaatg ccattaccca ggccgacgca gcagagcaag gagttgtgat caagcctgca
    10681 caaaattcca ccgttgctgt tgcgtcgcaa ccgacaatta cgtattctga gtcttcgggt
    10741 gaacagagtg ctaacaaaat gttgccgaac aacaagccaa ctgcgaccac ggtcacagcg
    10801 cctgtaacgg taccaacagc aagcacaacc gagccgactg tcagcagtac atcaaccagt
    10861 acgcctatct ccacctggcg ctggccgact gagggcaaag tgatcgaaac ctttggcgct
    10921 tctgaggggg gcaacaaggg gattgatatc gcaggcagca aaggacaggc aattatcgcg
    10981 accgcagatg gccgcgttgt ttatgctggt aacgcgctgc gcggctacgg taatctgatt
    11041 atcatcaaac ataatgatga ttacctgagt gcctacgccc ataacgacac aatgctggtc
    11101 cgggaacaac aagaagtgaa ggcggggcaa aaaatagcaa ccatgggtag caccggaacc
    11161 agttcaacac gcttgcattt tgaaattcgt tacaagggga aatccgtaaa cccgctgcgt
    11221 tatttgccgc agcgataaat cggcggaacc aggcttttgc ttgaatgttc cgtcaaggga
    11281 tcacgggtag gagccacctt atgagtcaga atacgctgaa agttcatgat ttaaatgaag
    11341 atgcggaatt tgatgagaac ggagttgagg tttttgacga aaaggcctta gtagaagagg
    11401 aacccagtga taacgatttg gccgaagagg aactgttatc gcagggagcc acacagcgtg
    11461 tgttggacgc gactcagctt taccttggtg agattggtta ttcaccactg ttaacggccg
    11521 aagaagaagt ttattttgcg cgtcgcgcac tgcgtggaga tgtcgcctct cgccgccgga
    11581 tgatcgagag taacttgcgt ctggtggtaa aaattgcccg ccgttatggc aatcgtggtc
    11641 tggcgttgct ggaccttatc gaagagggca acttggggct gatccgcgcg gtagagaagt
    11701 ttgacccgga acgtggtttc cgcttctcaa catacgcaac ctggtggatt cgccagacga
    11761 ttgaacgggc gattatgaac caaacccgta ctattcgttt gccgattcac atcgtaaagg
    11821 agctgaacgt ttacctgcga accgcacgtg agttgtccca taagctggac catgaaccaa
    11881 gtgcggaaga gatcgcagag caactggata agccagttga tgacgtcagc cgtatgcttc
    11941 gtcttaacga gcgcattacc tcggtagaca ccccgctggg tggtgattcc gaaaaagcgt
    12001 tgctggacat cctggccgat gaaaaagaga acggtccgga agataccacg caagatgacg
    12061 atatgaagca gagcatcgtc aaatggctgt tcgagctgaa cgccaaacag cgtgaagtac
    12121 tggcacgtcg attcggtttg ctggggtatg aagcggcaac actggaagat gtaggtcgtg
    12181 aaattggcct cacccgtgaa cgtgttcgcc agattcaggt tgaaggcctg cgccgtttgc
    12241 gcgaaatcct gcaaacgcag gggctgaata tcgaagcgct gttccgcaag taagtaagca
    12301 tctgtcagaa aggccagtct caagcgaggc tggccttttt cttttgtttg ggaagcttgt
    12361 gtttttgtcg cctggataag acacgtcagc gtcctacccg gcaatcagca ttctatgtgc
    12421 tcatcatctt gcgcaccaac tgcataaaca gctcctgctc ttctccactc aaacgcccca
    12481 aaaattcctc atccacgctg tcaccaatag gtatcgccgc agcaagtagc ttttctcctt
    12541 cggcagtcag ccagacaaag cgtcgccgct tgtcagcgac gtcatgttca cgtctgacta
    12601 agccacgatt ttccattctt gccaacattt ctgctagcgt tgctttggtg ctgactgctg
    12661 cttctatcag tgccacctgt tcaataccag gtttatcagc aatggcgcgc atcaccgcgt
    12721 actgtggttt ggtcaggtca ggtaactcgt gctgccagcg agccgtgtgc tgctgaaaaa
    12781 gctgtcgtaa ctgatggaac gctttatttc gtaacgccat gtaaactccg ggctaattct
    12841 ctgtcgctat catagcggtt ttttagtgac gaagaagatg ttgttacttt tcaatccttg
    12901 ccgctgtgga aatgaaggag tatgttaata ataacgttcg tatacgaata attaagagaa
    12961 taagcaatga aactgatcgt cgggatgaca ggggctaccg gtgcgcctct tggtgtggca
    13021 ttactgcaag cgctgcgtga gatgccgaat gtcgagactc atctggtgat gtcgaagtgg
    13081 gcgaaaacca ccattgaact ggaaacgcct tacagcgctc gcgatgttgc tgccctcgca
    13141 gacttcagcc ataacccggc ggatcaggcg gcgatcatct catccggttc ttttcgtacc
    13201 gacggcatga tcgttattcc gtgcagtatg aaaacgctcg ccggtatccg cgctggttac
    13261 gccgatggcc tggtagggcg cgcggcggac gtcgtgctca aagaaggccg caaactggtg
    13321 ctggtgccgc gtgaaatgcc gcttagcacc atccatctcg aaaatatgct cgcactttca
    13381 cgcatgggcg tggcgatggt gccgccgatg cctgcctttt ataaccatcc cgaaacggta
    13441 gatgacattg tccaccatgt ggtagcccgc gtgctggatc aatttggcct cgaacatccc
    13501 cacgccaggc gctggcaagg attgccgcag gcccggaatt tttctcagga gaatgaataa
    13561 tggcatttga tgatttacgc agctttttac aggcgcttga tgaccacggc cagttactga
    13621 aaatcagcga agaagtgaac gctgagccgg atctggcagc agcagctaac gccaccgggc
    13681 gtatcggcga cggcgcgccc gcgctgtggt ttgataatat tcgcggcttt accgatgccc
    13741 gcgtggcgat gaataccatc ggttcctggc agaaccacgc gatttccctt ggcctgccac
    13801 caaacacccc ggtcaaaaag cagattgatg agtttatccg ccgctgggat aacttcccga
    13861 ttgccccgga gcgccgcgcc aatccagcct gggcgcagaa caccgtcgat ggcgaagaga
    13921 tcaacctgtt cgatattctg ccgctgtttc gcctgaatga tggcgatggc ggtttctatc
    13981 tcgacaaagc gtgcgtggtt tcccgcgatc cgctcgaccc ggataacttc ggcaagcaga
    14041 acgtcggcat ctaccgcatg gaagtgaagg gcaagcgtaa gctcggcctg caaccggtgc
    14101 cgatgcacga tatcgccctg catctgcata aagcagaaga gcgcggtgaa gatctgccga
    14161 ttgcgatcac gctcggtaac gatccgatca tcacgctgat gggggccacg ccgctgaaat
    14221 acgatcagtc tgagtatgaa atggcaggcg cgctgcgtga aagcccgtac ccgatcgcca
    14281 ccgctccgct gaccggtttt gatgtgccgt ggggttcaga agtgatcctc gaaggggtta
    14341 tcgaaagccg taaacgcgaa atcgaagggc cgttcggtga gtttaccggg cactactccg
    14401 gtgggcgcaa catgaccgtg gtgcgcatcg ataaagtctc ttatcgcagc aaaccgattt
    14461 tcgaatcact ctatctcggt atgccgtgga ccgaaatcga ctacctatga ctaggtaatt
    14521 tattcaagat aaccgggaag agattaaaaa agacgggaat agattggaat caacaagtta
    14581 ggaactcaag gttttcaaga taaagcgagt ccaaaaatgt taaacggttc gatatcaata
    14641 aaacattcaa gataactcac aggttcttac aatagccttt aaagaccgtt caaaaacgat
    14701 tcaggtgttt attatagcgc tgatgggtgc aagcgccaaa accaatgtaa ttttagcaca
    14761 gctaggcact tgcactgggt tatgcgctgg ctgctttctg aggtagattt aagtgatcgc
    14821 ccttagcttc ggacaacgcg taaatctctc taaccttttc gtctggcaaa ttaccaaggc
    14881 ttaacgctat ggctctacga actccaacta gagcgtgcaa ctcttggatg tgctcatcca
    14941 gaagaatgag taattgctcc gctccttttc tcccaagaac atcagctact tgttgttttt
    15001 cttgtggttt aagaaatgac aagaacatta gaatttgagc atcaacatta tccgcattct
    15061 cttttttggg ttctttggaa tcttttagtt caacatcacc ctctcccgtc atgagccatt
    15121 ccaaggtaac attttcgctt tctgctatct tttttaactg atacgggcgt ggtatcggtg
    15181 cgtaatcccg cctgttgtaa taatttttta aagtgccttc attgatcccc catgcccgag
    15241 cagccgcatt tcttgaaggg tatcgttcta ctaacctaaa gattctttcg ataatcggtt
    15301 cttttccatc ggcaggaaaa gaaccttggg gtgcttttct cattcttggt tcctttctat
    15361 ctaattcatt gataaataat aaaattaaaa aataaaccat aaaaacacca aagaaccctt
    15421 tgcagggaat ctttggtatc ttaatcttta tccatacgga tatccgaccg gatatccgcg
    15481 cgggtaattt tgtaggttaa aggaaagatg agacaaagag aagatagtga ccgcgattgg
    15541 ccttcgctga agattcagct ggcgctcaaa gataacgggt ggtcttcctt ccgggagctt
    15601 tcactccatg ctggttatgc tcaggattca ctgaaagcgg tagttcgcac accaaacaaa
    15661 aaatacgaac gcgtaatttc tgacgctata ggtgtaccgc cagaaatcat ctggcccagc
    15721 cggtatcgta acgatggatt tatcaggaag gcgtcgtgat atgcagtcgt acgttaccgt
    15781 aaatgacctg ctcggcgtac caggaatgcc agccaccaca aaaggcatcc gacaggcgtt
    15841 acagcgtttt tctcgtgatc ttggtgacgt tagtcgccgt cgggaaggca caaaagccat
    15901 tgagtaccac attgattgtc tgcctgaaat aacacgaaag gcactgcgtg aacgctatgt
    15961 ggaacagctg gtggctacag agaacaatgt ttctgaagtg aaagctgtta ccagaaaaac
    16021 acgcaatcct gacgctgtcc aggcaatcga agcatatcgc ggttctccac aactgatgga
    16081 agaacgcctg aatgcgctga ccgaaaacca gcgctgggta tctgaagcaa gagctgcgct
    16141 ggtggtggaa gtgctgaagc tggaaagcgc cggtaacccc gggcgactga aagccattaa
    16201 ctttcttgtt gaaaaagccc gtaaaggtga gctgccggag cgcctgcaac aggccgcagt
    16261 taacgccaat gcaaaacgtg gcgctaatcg tactatcagc cgcgatccac tttaccagtg
    16321 ggttctgaaa tacaaccagt cgcagaacgc cgccgaacgg ctgctgttac tggcaccggg
    16381 aaaacgcgac gaaataaaac cggaagaaat cagctggctg cctgaatttc tggcgcagta
    16441 tcgccaggtg aacggcagac ccatgtcaga agcctacgag gattttgtcg cagaatggca
    16501 gcgtcgccat gcggacgaac cgtacatgct ggaggtgatg ccttcctatg atgttgtccg
    16561 gtatgccatg aaaaaactgc cggaagtggt gaaacaaaaa gggcgcgtta ccggcagtga
    16621 ataccgtcag cttgaaggat tcacccgccg cgactggaca gctatgccgg tgaattatgt
    16681 ctggattggt gacggtcacg gtatgaagct gaaatgtgcg catccgatcc acgggcgtcc
    16741 attctcaccg gaagtcacgt ttgtgatcga tggtggaaca cggtttgttg ttggctggag
    16801 tcttgacctt gccgaaaacg tgttcgccgt tgccggtgcg atacagcacg gcattcgcaa
    16861 ccacggaaaa ccgttcctgt attactcgga taacggttcc ggtgaaacgg ctgacatgct
    16921 ggacaaagag gtcgtgggta ttctgccacg cctggggatt aaacacccta ccggtattgc
    16981 cggtaatcca cagggacgcg gcattattga acgcctgaac cggacactgc cgatgcgtat
    17041 agcgaggagg tatcgcacct attttggcaa gggtgctgac cgggaatcgt tgcgtgtact
    17101 taaccgtgat ctgcgttcag cgtttaacgc cctgcaacag gacaagcctc tgaacgaccg
    17161 ccagaaagcg gcaatgcgcg agctgccatc gtgggcggag cttattgagg ctattcgtga
    17221 aggcgtggaa tggtacaaca accgcccgca ttctgagctg ccgatgaaac cgaacggacg
    17281 gcattacagc ccgacagagt tcagaaaaaa acgccaggca gaagaagaca ccgaaattga
    17341 gtggctgagt gatcttgagc tgcgcgacat gttccgccca atggtggaac gaccggtaag
    17401 acgctgcgaa attcagtggc tgaacaatat ttactacgcg ccggagttgc gtgacgaaca
    17461 cggtcgcaaa gtgcttatca gttatgacat tcatgacgcc gagcgaatta cggtacgtcg
    17521 caaggacggc agctttatct gtgaggcgat atggaacggc aataaacgcg cagcctttgc
    17581 tgtcagtgct gaatatcaca aacaacagca gcgtattaaa ggaatgcgca aacgcgctga
    17641 ggaaaaaatt cgtgatgctg aagatgaagg tattcagatt ctggaacaca aacaggctga
    17701 accctggctg agtaatgttt atcgcccggt tggtaatgtg gtggcagttc agcaaccgga
    17761 atatgaagaa gagcatgacg aagaatttga acgcgatttc cggctgggga tgcaaaaact
    17821 ttttgccatg caggaagaag atgatccgct ggcctgaata aaaaaacagt ccgcgggaac
    17881 ggactgttaa ataaaggaaa caaagctgat ttgaaataac cgaggaaata ctatgactga
    17941 tattgatgat gtttgaggtg tactggcaat agcggacact accatttgtt ctttttttaa
    18001 gcagccatct gatgatattt ttccctgaag gctgccgggg agata
//
LOCUS       DAZPCS010000072        17917 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZPCS010000072 DAZPCS010000000
VERSION     DAZPCS010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17917
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.072"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            105..629
                     /locus_tag="ACHUQ7_002345"
     CDS             105..629
                     /locus_tag="ACHUQ7_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HFR1135728.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSVQS
                     GDVLSSLVMRVAQD"
     gene            710..1384
                     /locus_tag="ACHUQ7_002346"
     CDS             710..1384
                     /locus_tag="ACHUQ7_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFR1135729.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLYIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNIKSNNANNWYLTIINDHGN
                     YISDKI"
     gene            1400..3862
                     /locus_tag="ACHUQ7_002347"
     CDS             1400..3862
                     /locus_tag="ACHUQ7_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFR1135730.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYSWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNNKSTYVRFNSGLNLLEWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQFLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGASKQSDFVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGWNTRIGA
                     ISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRTFNDHVW
                     ANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLWRDYWGR
                     SGSSKDYQLSYSNNWRRISYTLAASQAYGENHHEEKRFNIFISIPCDWGDDVTTPRRQ
                     IYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANLTWNAPV
                     ATVNGSYSQSSTYRQTGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDAYVNGQK
                     YRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVLVNFDTD
                     QRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVNVAIDKQ
                     QGLSCTITFGKEIDESRNYICQ"
     gene            3878..4911
                     /locus_tag="ACHUQ7_002348"
                     /pseudo
     CDS             3878..4911
                     /locus_tag="ACHUQ7_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(4993..5331)
                     /gene="rcnB"
                     /locus_tag="ACHUQ7_002349"
     CDS             complement(4993..5331)
                     /gene="rcnB"
                     /locus_tag="ACHUQ7_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFR1135731.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(5550..6380)
                     /gene="rcnA"
                     /locus_tag="ACHUQ7_002350"
                     /pseudo
     CDS             complement(5550..6380)
                     /gene="rcnA"
                     /locus_tag="ACHUQ7_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
     gene            6502..6774
                     /gene="rcnR"
                     /locus_tag="ACHUQ7_002351"
     CDS             6502..6774
                     /gene="rcnR"
                     /locus_tag="ACHUQ7_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFR1135732.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      6866..6962
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6997..7785
                     /gene="thiM"
                     /locus_tag="ACHUQ7_002352"
     CDS             6997..7785
                     /gene="thiM"
                     /locus_tag="ACHUQ7_002352"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFR1135733.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            7782..8582
                     /gene="thiD"
                     /locus_tag="ACHUQ7_002353"
     CDS             7782..8582
                     /gene="thiD"
                     /locus_tag="ACHUQ7_002353"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFR1135734.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKIWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            8647..9464
                     /locus_tag="ACHUQ7_002354"
                     /pseudo
     CDS             8647..9464
                     /locus_tag="ACHUQ7_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
     gene            9516..10262
                     /locus_tag="ACHUQ7_002355"
     CDS             9516..10262
                     /locus_tag="ACHUQ7_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFR1135735.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAAEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(10236..11201)
                     /locus_tag="ACHUQ7_002356"
     CDS             complement(10236..11201)
                     /locus_tag="ACHUQ7_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFR1135736.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFCPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(11198..12201)
                     /locus_tag="ACHUQ7_002357"
                     /pseudo
     CDS             complement(11198..12201)
                     /locus_tag="ACHUQ7_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            12241..13293
                     /gene="fbaB"
                     /locus_tag="ACHUQ7_002358"
     CDS             12241..13293
                     /gene="fbaB"
                     /locus_tag="ACHUQ7_002358"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFR1135737.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            13601..14455
                     /gene="gatY"
                     /locus_tag="ACHUQ7_002359"
     CDS             13601..14455
                     /gene="gatY"
                     /locus_tag="ACHUQ7_002359"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFR1135738.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEVDALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFLQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            14484..15746
                     /gene="gatZ"
                     /locus_tag="ACHUQ7_002360"
     CDS             14484..15746
                     /gene="gatZ"
                     /locus_tag="ACHUQ7_002360"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HFR1135739.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDTIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAMPHQLIMNKIYDVLRAYRYGCAE"
     gene            15756..16208
                     /gene="gatA"
                     /locus_tag="ACHUQ7_002361"
     CDS             15756..16208
                     /gene="gatA"
                     /locus_tag="ACHUQ7_002361"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFR1135740.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            16239..16523
                     /gene="gatB"
                     /locus_tag="ACHUQ7_002362"
     CDS             16239..16523
                     /gene="gatB"
                     /locus_tag="ACHUQ7_002362"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFR1135741.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            16527..17882
                     /locus_tag="ACHUQ7_002363"
     CDS             16527..17882
                     /locus_tag="ACHUQ7_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFR1135742.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRMIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQPAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFTKQEKAVLA
                     E"
BASE COUNT         4672 a         4099 c         4558 g         4588 t
ORIGIN      
        1 aaacaatatg tttacattgc ggcagctttt ttgatgtgag aaatttgttt ccaagagata
       61 cagactctta acaaccaaaa ataaatttta ttaaggaaca tattatgaaa cgttcaatta
      121 ttgctgccgc tgtcttttct tcttttttta tgagcgctgg agtatttgct gcagacgttg
      181 ataccggaac attaaccatt aaaggaaata ttgcagaatc tccgtgtaaa ttcgaagcgg
      241 gtggtgattc agtaagtatt aatatgccga ctgtaccaac cactgtcttt gaaggtaaag
      301 ctaaatattc tacctatgat gatgcagtcg gtgtaaccag cagcatgtta aaaattagct
      361 gcccgaaaga agttgctggt gtaaaactct cgttgatcac aaacgacaaa ataaccggta
      421 acgataaggc gatagccagt agcaacgata ccgtaggtga taacagcgat gtcctagatg
      481 tttctgcacc ttttaacatt gagagttata aaacagcgga aggtcaatat gctattccgt
      541 ttaaagcaaa atacctgaaa ctgacagata actcagtgca atcaggtgat gtgttatctt
      601 ctctggttat gcgtgtggcg caggattaat aaataaaatt gcaagtttct ccaggtaata
      661 aacatggccg ctatccccat ggcggctttt taatttaaga ggcattaaga tgaaaggatt
      721 attatcttta ctcatttttt ctatggtcct tcctgcacat gccggaattg ttatctacgg
      781 gacgcgcatt atttacccgg cagaaaataa agaagttatg gtgcagttga tgaaccaggg
      841 aaatcgttct tcgctgctgc aggcgtggat tgatgatggc gatacgtcgt taccaccaga
      901 aaaaattcag gttcctttca tgttaacgcc accagtggca aaaatagggg caaattccgg
      961 gcagcaagta aaaatcaaaa ttatgccgaa taaactgccc actaataaag aaagcatttt
     1021 ttatctgaat gttctgtata ttccaccaaa tagtccagag caagaaggca agaatgcact
     1081 gaagtttgcg atgcaaaaca gaattaagtt gttttaccgg ccagcgggta ttgctccggt
     1141 aaataaagcg acctttaaaa agttactggt aaatcgcagt ggcaatggat tggtgataaa
     1201 aaatgactca gctaattggg tgacgatttc ggatgtcaaa gctaataatg ttaaagtcaa
     1261 ttatgaaact attatgattg cccccttaga aagtcagagt gttaatatca aaagtaataa
     1321 tgcaaataac tggtatctga ccattatcaa tgaccacggc aactatatta gtgacaaaat
     1381 ttaattgcag gagctgccta tgttgagaat gaccccactt gcatcagcaa tcgtagcgtt
     1441 attgctcggc attgaagctt atgcagctga agaaaccttt gatacccatt ttatgatagg
     1501 tggaatgaaa gaccagcagg ttgcaaatat tcgtcttgat gataatcaac ccttaccggg
     1561 gcagtatgac atcgatattt atgtcaataa gcaatggcgc gggaaatatg agattattgt
     1621 taaagacaac ccgcaagaaa catgtttatc aagagaagtt atcaagcggt taggcattaa
     1681 tagcgataac ttcgccagcg gtaagcaatg tttaacattt gagcaacttg ttcagggtgg
     1741 gagctatagc tgggatatcg gggtttttcg tctcgatttc agtgtcccgc aggcttgggt
     1801 ggaagaactg gaaagtggct atgttccacc ggaaaactgg gagcggggta ttaatgcgtt
     1861 ttatacttct tattatgtga gtcagtatta cagcgactat aaagcgtcgg gtaacaacaa
     1921 gagtacatat gtacgtttta acagcgggtt aaatttactg gagtggcaac tgcattctga
     1981 tgccagtttc agtaaaacaa ataacaatcc aggggtgtgg aaaagcaata ccctgtatct
     2041 ggaacgtgga tttgcccaat ttctcggcac gcttcgcgtg ggtgatatgt acacatcaag
     2101 cgatattttt gattctgttc gcttcagcgg tgtgcggttg tttcgtgata tgcagatgtt
     2161 gcctaactcg aaacaaaatt ttacgccacg ggtgcagggg attgctcaga gtaacgcgct
     2221 ggtaactatt gaacagaatg gttttgtggt ttatcagaaa gaggttcctc ctggcccgtt
     2281 tgcgattaca gatttgcagt tggccggtgg tggagcagat cttgatgtta gcgtgaaaga
     2341 ggcggacggc tcggtaacca cctatctggt gccttatgca gcggtgccaa atatgctgca
     2401 acccggcgtg tcgaaatatg attttgcggc gggggcgagc aagcaaagtg attttgtcca
     2461 ggcaggttat cagtatggtt ttaataactt attgacgctg tatggcggca cgatggttgc
     2521 taataattac tatgcgttta ccctcggaac gggttggaac acgcgcattg gcgcaatttc
     2581 cgtcgatgcc acgaagtcgc atagtaaaca agacaacggc gatgtgtttg acgggcaaag
     2641 ttatcaaatt gcctacaaca aatttgtgag ccaaacgtcg acgcgttttg gtctggcggc
     2701 ctggcgttat tcgtcgcgtg attaccggac atttaacgat cacgtttggg caaacaataa
     2761 agataattat cgccgtgatg aaaacgatat ctatgacatt gccgattatt accagaacga
     2821 ttttggccgt aaaaatagct tttctgccaa tatgagccag tcattgccag aaggctgggg
     2881 ttctgtgtcc ttaagtacgt tatggcgaga ttactggggg cgtagcggca gcagtaagga
     2941 ttatcagttg agttattcca ataactggcg gcggataagc tataccctcg cggcaagcca
     3001 ggcgtatggc gagaatcatc atgaagagaa acgttttaat atttttatat caattccctg
     3061 tgattggggt gatgacgtta cgacgcctcg tcggcaaata tatatgtcta actcaacgac
     3121 gtttgatgat cagggttttg cctcaaataa tacgggatta tcaggaacag tagggagtcg
     3181 ggatcagttc aattatggtg tcaacctgag tcatcaacat cagggaaatg aaacgacagc
     3241 tggggcgaat ttgacctgga acgcgccggt tgcgacagtg aatggcagtt atagtcagtc
     3301 gagtacttat cgacagactg gagccagtgt ttcagggggc attgtcgcct ggtcgggtgg
     3361 cgttaatctg gcgaaccgtc tttccgaaac gtttgctgtg atgaatgcgc caggaattaa
     3421 agatgcttat gtcaatgggc aaaaatatcg cacaacaaac cgtaatggag tggtggtata
     3481 cgacggaatg acaccttatc gggaaaatca cctgatgctg gatgtgtcgc aaagcgatag
     3541 cgaagcagaa ttacgtggca accggaaaat tgccgcccct tatcgcggcg cggttgtact
     3601 ggttaatttt gataccgatc agcgcaagcc ctggtttata aaagcgttaa gagcggatgg
     3661 tcaaccatta acgtttggtt atgaagtcaa tgatatccat ggtcataata ttggcgttgt
     3721 cggccaggga agccagttat ttattcgcac caatgaaata ccgccatcgg ttaatgtagc
     3781 aattgataag caacaaggac tttcatgcac aatcaccttc ggtaaagaga ttgatgaaag
     3841 tagaaattat atttgccagt aatagtcagg tggttctatg gaaattcgca taatgctatt
     3901 tatattaatg atgatggtta tgcctgtgag ctatgcggca tgttatagtg agttatctgt
     3961 tcagcacaat ttggttgttc aggggaattt tgcacttact caaacacaaa tagcgacata
     4021 tgagcataat tttaatgatt cgtcatgcgt gagtacaaat actatcaccc ctatgagccc
     4081 atcggatatt attgttggac tttataacga taccattaaa ttaaatttac attttgaatg
     4141 gaccaataaa aacaacatca cgttgtcaaa taatcagagc agtttcacca gtggttattc
     4201 ggtaacggtg acacctgcgg ccagtaatgc aaaagtgaat atttctgcgg ggggcggtgg
     4261 ttcagtgatg attaatggtg ttgcgacatt atccagtgct tcatcatcga cacgcgggag
     4321 tgccgcagta caatttctac tgtgtttatt aggtggcaag tcatggggtg catgtgtaaa
     4381 tagctacaga aatgcattgg cacaaaatgc aggtgtctat tccttcaatc tgacattgtc
     4441 atacaacccg ataaccacaa cctgcaaaca ggacgattta ttaattactt tagacagtat
     4501 tcccgtttta caattaccag ccacaggtaa caaagcaaca ataaatagta aaaaaaggga
     4561 tattattctg cgttgtaaaa atttattagg tcaacaaaat caaacatcac ggaaaatgca
     4621 ggtgtattta tcaagttctg acttgttaac caacagcaac acaatactga aaggtgcgga
     4681 agataatgcg taggatttat tcttgaaagt aatggttcgc cagtcacact tttaaatatc
     4741 actaacagca gtaaaggata cacaaattta aaagaaattg cggcaaagtc aaaacttaca
     4801 gatacaacgg tttcaattcc gataacagcc agttactacg tctacgatac aaacaaagtt
     4861 aaatctggcg cactggaggc aaccgcatta atcaacgtga aatacgacta ataacaagca
     4921 aaacgagcaa gtggctaata ataaaaaata acaaggtgtc tgcatttccc cctcatgatg
     4981 agggggcttt ttttagcgat gataaaaaat ctcaccgtcg taggctttaa tgattttacc
     5041 gtcggtgtcg ctgatcaaca cgtacgcgcc acccatatag gtccagtgcg tcccggcatc
     5101 aggcgcgggc aggttacgta actgccactg cttaatgttg tactcatcgg tgcggtacat
     5161 ctctgggacg gtatcacctg gtttgaaatg cttaaagtca gcaatgaaac tacttaactc
     5221 atacttagaa attcccgagg tattggtcga acccgcggtg gctggtgcgg cccaggcggc
     5281 actggtaacc agcaaaagca cacccagcaa catcttattt ttaatggtca taaactctcc
     5341 actttgggga tctgtaatat cagtcgcata tctttcctgt catacaatat aactgacaag
     5401 cgatattttg ttagtgatcc catgatttcg taaaaagtgt aaatgaaagg atgccctaaa
     5461 agcatcctta gcgagcagag agtcagcgga aatagctgat ttaagaatca tcctgggctg
     5521 aagataacat gcaagcctaa aggattgagt tatcgcatta tgcccatgaa gccatgtacg
     5581 cccatataca caccgactaa gccaatcaac agactggaaa aatagggggc gcgtttagcg
     5641 agagtgttaa atccgctcca gcgttttgcg acctgctgaa cgctgattgc tgcgccaacg
     5701 cctacggtga caagcgttaa cgccaggcca atgctgaaac tgacgaccag tgttgcgccc
     5761 agtgtcaggg ctttcaactg aatgcatatc aacagcacgg taattgctgc cgggcagggg
     5821 ataaagccac cggttaagcc aaataacaaa atttgccagt tgatgacctc tctaccatca
     5881 aagcgtcgtt taatgtcatt ggcatgggct cgtgcatggg catcctgata ctcgccatgt
     5941 tcgtgatggt gatgatgtcc ttggtcgtgg tgatgttcgt gatcgtgatg atggtgatga
     6001 tgctcataat catgcccgtg catattctcc agccagttgc gttcgccgcg ctaggtacgc
     6061 cagaacatcc agaacgcggt gccaataatg atcactgcgg aaatcagctg gagccacggt
     6121 tctgctgatt gagcagtaaa gcgcttactg atcaccatcc cgccaaaggc aatcaaccag
     6181 accaccgcgg tatgcgaaat ggttgctgcc agtccgagca tcaccgcctg tttaatggtg
     6241 cctttgatgg cgatgataaa cgccgccatc atcgtttttg agtgccctgg ttccaggcca
     6301 tgaagcgcac caagtaagat ggcgctgggg atgaagaacc aggcgtttcc ttgctgaaga
     6361 agagttgtaa attcggtcat gataataatt cttagtatta attcggcaat ctgattctac
     6421 tcccccccag tacctgatac taccccccca gtagattaat agtgctatga tttttcatat
     6481 tcttgttaac caggtgttgc catgtctcat acaatccgtg ataaacagaa actgaaagcg
     6541 cgtgccagta agattcaggg ccaggtcgtg gcgctcaaga aaatgctcga cgagccgcac
     6601 gaatgcgctg cagttttaca acagattgct gctatccgtg gagcggtaaa cggtctgatg
     6661 cgggaagtga ttaaaggtca tctgacggaa cacatcgttc accaggggga tgagctaaaa
     6721 cgtgaagaag atctggatgt cgttctgaag gtgctggatt catatatcaa ataatttatt
     6781 aacgcgattg taaaactgcc gtttttcctc gtttacaacg ggggcgctgg acattaccat
     6841 cctcctctgc gatttatcat cgcaaccaaa cgactcgggg tgcccttctg cgtgaaggct
     6901 gagaaatacc cgtatcacct gatctggata atgccagcgt agggaagtca cggaccacca
     6961 ggtcattgct tcttcacgtt atggcaggag caaactatgc aagtcgacct gctgagttca
     7021 gcgcaatctg cgcacgcgtt acaccttttt caccaacatt cccctcttgt gcactgcatg
     7081 accaatgatg tggtgcaaac ctttaccgcc aataccttgc tggcgctcgg tgcatcgcca
     7141 gcgatggtta tcgaaaccga agaggccagt cagtttgcgg ctatcgccag tgccttgttg
     7201 attaacgttg gcacactgac gcagccacgc gcacaggcga tgcgtgctgc cgttgagcaa
     7261 gcaaaaagct ctcaaacacg ctggacgctt gatccagtag cggttggtgc gctcgattat
     7321 cgccgccatt tttgtcatga acttttatct tttaaaccgg cagcgatacg tggtaatgct
     7381 tcggaaatca tggcattagc tggcattgct aatggcggac ggggagtgga taccactgac
     7441 gccgcagcta acgcgatacc cgctgcacaa acactggcac gggaaactgg cgcaatcgtc
     7501 gtggtcactg gcgaggtgga ttatgttacc gatggacatc gtatcattgg tattcacggt
     7561 ggcgatccgt taatgaccaa agtggtagga actggctgtg cattatcggc ggttgtcgct
     7621 gcctgctgtg cgttaccagg cgatacgctg gaaaatgtcg catctgcctg tcactggatg
     7681 aaacaagccg gagaacgcgc agtcgccaga agcgaggggc caggcagttt tgttccacat
     7741 ttccttgatg cgctctggca attgacgcag gaggtgcagg catgaaacga attaacgctc
     7801 tgacgattgc cggtactgat ccgagtggtg gtgcggggat tcaggccgat cttaaaacct
     7861 tctcggcact tggcgcttat ggttgctcag ttattactgc actggtggcg caaaataccc
     7921 gtggcgtaca gtcggtgtat cgcattgagc ctgattttgt cgccgcccag ctcgattcgg
     7981 tgttcagcga tgtgcgaatc gataccacta aaatcggtat gttggcggaa accgatattg
     8041 ttgaagcggt ggcagaacgg ttgcaacgtt atcagatcca aaacgtggta ctcgacaccg
     8101 ttatgctggc aaaaagcggc gacccgctgc tttcaccttc ggcggttgct acgctgcgca
     8161 gtcgattatt gccacaggtt tcattaataa cgccaaactt gcccgaagct gccgccttgc
     8221 tcgacgcgcc acacgcgcgc accgaacagg aaatgctgga acaagggcga tcgctgttgg
     8281 cgatgggctg tggcgcagtg ctaatgaaag gtggtcatct ggatgatgag caaagcccgg
     8341 actggctgtt tacccgcgag ggtgaacaac ggtttaccgc accgcgcatt atgaccaaaa
     8401 acacccacgg cactggttgt acactctctg cggcgttggc tgcactacgc ccgcgccata
     8461 caaactgggc tgacaccgta caggaggcaa aaatctggct ttcatcggcg ttagcccagg
     8521 ccgacacgct ggaagttggt cacggtattg gtccggttca ccacttccac gcctggtggt
     8581 gagcaccgta ctatagctgc tttggttatt gtcagccttt ggcactgaca ttcagggatg
     8641 aaaccaatgc agctaagaat taccagtcga aaaaaactca ccgctctatt atgcgctctt
     8701 gggctaattt ccatcgttgc aatttatccg cgtcagacgg tgaatttttt ctattcgaca
     8761 gcagttcaga ttacagacta catccacttc tacggttatc gcccggttaa atctttcgct
     8821 attcgcattc ctgccagtta caccattcac ggattagatg tttcgcgctg gcaggagcgt
     8881 atcgactggc agcgggtggc aaaaatgcgc gacaacgata tccgcttaca gtttgctttt
     8941 attaaggcta cggaaggcga aaagctggtg gacccctatt tttcgcgtaa ctggcaatta
     9001 agccgcgaaa atggcctgct gcgcggggcg tatcattatt tctccccgtc ggtatccgct
     9061 tcagttcagg cgcgattatt tctgcaaacg gtggatttct cacaaggcga tttccctgcc
     9121 gtgctggacg tagaagaacg gggaaaatta tcggcaaaag aattacgcaa gcgggtaagt
     9181 cagtggctaa aaatggtcga aaaacgcacg ggaaaaaagc cgattattta ctcaggagcc
     9241 gttttttatc acaccaatct ggcgggctat ttcaatgaat atccgtggtg ggtggctcac
     9301 tattatcaac gtcgtccgga caatgacggc atggcctggc gcttctggca gcattccgac
     9361 cgtggacagg tagatggcat caatggtccg gtggatttta atgtgtttaa tggcacgggg
     9421 atgagttgca ggcattcgtt gatgggatta aagaaacgcc ttaaataaga agcagtgata
     9481 tactcccacc agactccctg gcaggaaaaa gacaaatgga acaagcgcat acccagctga
     9541 ttgcccaact gaacgagcgt attttagcgg cggataacac gccgctttat attaagtttg
     9601 ccgaaacggt aaaaaatgcc gtgcgcagcg gggtgctgga gcatggcaat attttgcccg
     9661 gtgagcgtga tttaagtcag ttaaccggcg tttcgcgcat tacggtgcgc aaggcgatgc
     9721 aggcgctgga agaagagggc gtggtgacgc gttcgcgcgg ttacggcacg cagatcaaca
     9781 acatcttcga atactcgttg aaagaagcga gaggtttttc tcagcaggtg gtattgcgcg
     9841 ggaaaaagcc cgatacgcta tgggttaata agcgtgtcgt gaaatgtccc gaagaagttg
     9901 cgcagcagct ggcggccgaa gcgggaagtg atgtcttttt gcttaagcgt attcgctatg
     9961 tcgatgaaga agcggtatcg attgaggaat cgtgggttcc ggcgcattta attcatgatg
    10021 ttgatgccat cggaatttct ctttatgact atttccgcag ccagcacatt tacccacaac
    10081 gtacgcgttc ccgcgttagc gcccggatgc cggatgccga gtttcagtca catattcagt
    10141 tagatagcaa aataccggtg ctggtgatca agcaagtggc gcttgaccaa cagcaacggc
    10201 ctattgagta cagcattagc cactgtcgca gcgatctata cgtttttgtg tgcgaggagt
    10261 agttcctcgc gcgttggcgc acaatcaccg ccccgatgcc cgacaaccca cgacgccact
    10321 gcattgccca gtaatacggc atccgccagt ggcagaccag aggccagccc ggcaagtacg
    10381 ccacctgcat gactgtcgcc cgccccaatg gtgtctacaa cttgcgttgg aaatgccgga
    10441 atgcagccag aagcgtcgtt gctgaaatac catgcgcctt ctttatcgag acgaacgatc
    10501 aacggcgcgg caaatttctc ctgccattgc ttgccaagtg ttgttatctc tgcggataaa
    10561 gcaaaacgtt cggcggcaat ctcagcctct tgacgattga gtgacactaa aggtcgacag
    10621 gccatgatcc gcgccagtaa tgcgtccggg atatcgccaa tacgtgggca aaaatcgata
    10681 aacggcgtca cgtcttgcag cttttccagc cattccacta acaattcgcc gcagggcgag
    10741 gccagttgat aaccggaaaa atagagcagg ttgccaggcg caacggttaa tcgtgccaac
    10801 cattggcgat tccattgatt ttccacaccg ctgaatgaca taaaagtgcg ttcgccatcc
    10861 ggctcaacca gtgccagaca ccagccgtta tcgctttcgg cgttatcgat caggctgatt
    10921 aacccctctt tcgccatccg gttgcgaata atctccgccc acacgccctg accgagcggc
    10981 aaggcattac ccgcttcgat gccgaggcgc tttaacgcca cggcaatatt cagtgcgcag
    11041 ccgccaacgt taacgctctg ctgtttcagt tcgatatcgc acccacgcca ggggagggca
    11101 taagcgtcgg cgatcacgtc aatgaccgcc gcgccgacga ccatcaccgg ctgacgcgtg
    11161 gttaattcag gcagcagtgt gtgtaatcga gcgccgctca taccgcctcc cgttgttgac
    11221 gatatttcgc cagcgctgtg gcatagcggt tgaaatcaag ctgatttacc gcatccagct
    11281 ccgcctttaa cgcaggatcg acagcgttaa tgccatgcaa cgcgccgcaa atcgccgtcg
    11341 ccatagcacc tatggtgtct gtgtcgccgc caagattggc gcacaggacg gcgcagcggt
    11401 ttgggtcggt ttgtgccagt tcaactagcg caatggcgca cggaacggac tcaatagtgc
    11461 tggtgcctgc gccaacgacc tggtaaagct gttcgctggc ggattcggtg ccgtcggcat
    11521 tacgcacaat tttcagcgca atctccagac gtgccgccag tgaggcgctg aacgtggtgg
    11581 tgcgtttttg ctgtgcatgt cgcgcaattg aaggcaatga atcaacaatc gctgaccagc
    11641 gttctccgtc aatggcacga gaaatcgccc atgcgatgac taccgcgcct gcaaccgcca
    11701 gatcggattt atgggtcggg ctggacgcca gcgccacatc atcaataaag gaatcaacat
    11761 cacgcgctgg aagcaaacaa cctaatggcg agacgcgcat cgctgcgccg ttggttacgc
    11821 cgttgttttc cagttcagca acgggtttac cgtcgcgaat ggcgttaagc gcaatcttcg
    11881 aggtcggacc tagtacgttt ttgttaaagg cgtcgaaacg caacgcccag tcgagaatat
    11941 tacgcccaat cagattcgga tcgatcttgc cttcacattc cagtaacgca tccgccagac
    12001 acaggccatc gaggtatcgt cggtgaattc ggcgcggtta aaataacagg ccgcgttatt
    12061 ctcctttggt ccaggcagaa aacggtcaat ccagccaaag tgtgctttaa cgcggctgcg
    12121 tggccaaagc tcggagggca tccccatcgc atcccctaac gcctgcccat aaagagcacc
    12181 gagaatacgt tctgttttca tttaacttcc ccttgaatcg gaatttacgg gagcatagta
    12241 atgacagata ttgcgcagtt gcttggcaaa gacgccgaca accttttaca gcaccgttgt
    12301 atgactattc cttctgacca gctttatctc cccggacatg actacgtaga ccgcgtgatg
    12361 attgacaata atcgcccgcc agcggtgtta cgtaatatgc agacgttgta caacaccggg
    12421 cgtctggctg gcacaggata tctttctatt ctgccggttg accagggcgt tgagcactct
    12481 gccggagctt catttgctgc taacccgctc tactttgacc cgaaaaacat tgttgaactg
    12541 gcgatcgaag cgggctgtaa ctgtgtggca tcaacttacg gcgtgttggt gtcggtatcg
    12601 cggcgctatg cgcatcgcat tccatttctc gtcaaactta atcacaacga gacgctaagt
    12661 tacccgaata cctacgatca aacgctgtat gccagcgtgg agcaggcctt caacatgggc
    12721 gcggtggcgg ttggtgcgac tatctatttt ggctcggaag agtcacgtcg ccagattgaa
    12781 gaaatttctg cggcctttga acgcgcgcac gagctgggca tggtgacagt gctgtgggcc
    12841 tatttgcgaa actccgcctt taagaaagat ggcgttgatt accatgtttc cgccgacctg
    12901 actggtcagg cgaaccatct ggcggcaacc ataggtgcag atatcgtcaa acaaaaaatg
    12961 gcggaaaata acggcggcta taaagcgatt aattacggtt ataccgacga tcgcgtgtac
    13021 agcaagttaa ccagcgaaaa cccgattgat ctggtgcgtt atcagttagc taactgctat
    13081 atgggccggg ctgggttgat aaactccggc ggtgctgcag gcggtgaaac tgacctcagc
    13141 gatgcagtgc gtactgcggt tatcaacaaa cgcgcaggcg gaatggggct gattcttgga
    13201 cgtaaggcgt tcaagaaatc gatggctgac ggcgtgaaac tgattaatgc cgtgcaggac
    13261 gtttatctcg atagcaaaat tactattgcc tgatacgcct catacgaaca gcaccacccc
    13321 gtaggacgga taagacgttt acactgcatc cggcaaccta tgcctgatgc gacgcggaag
    13381 cgtcttatca tgcctacata gcactgccac gtatgtttac accgcatccg gcataaaaac
    13441 acgcgcactt tgctacggct tccctatcgg gaggccgttt ttttgccttt cactcctcga
    13501 acaattttca tattgttgtt tttgcgatca ttatctcgac atttaaaagc aaataatttc
    13561 attatatttt gaaatcgaaa gcaaacgaca agatatgaaa atgtacgtgg tatcgacaaa
    13621 gcagatgctg aacaacgcac agcgcggcgg ttatgcggtt ccggcattca atattcacaa
    13681 tctcgaaacg atgcaagtgg tggtggaaac cgctgccaac ctgcatgcgc cggtcatcat
    13741 cgccggaacg cctggcacat ttactcatgc tggtacagaa aatctgttgg cgctggtcag
    13801 cgcgatggcg aagcaatatc accatccact ggcaattcat ctcgaccatc acacgaaatt
    13861 tgacgatatc gctcagaagg ttcgttctgg cgtgcgctca gtcatgattg acgcctcgca
    13921 tttgcctttt gcgcaaaata tttcacgggt caaagaggtg gtggattttt gccatcgctt
    13981 tgatgtcagc gtcgaggcgg agctggggca acttggcggc caggaagatg atgtgcaagt
    14041 caatgaagtc gatgcgttgt acaccaaccc cgcacaggcg cgtgaatttg ccgaggcaac
    14101 cgggattgat tccctggcgg tcgccatcgg cacggctcac ggtatgtatg ccagcgcacc
    14161 ggtgcttgat ttttctcgac tggagaacat tcgccagtgg gtgaacttac cgctggtgct
    14221 gcatggcgcg tcaggattat cgactaagga tattcagcaa accatcaaac tggggatatg
    14281 caaaatcaac gttgcaacgg agctgaaaaa tgccttcttg cagtcgttaa aaaattacct
    14341 gaccgagcac cccgaagcga ccgatccccg ggattatttg cagtcggcta aatccgcaat
    14401 gcgcgatgtg gtgagcaaag tgattgccga ttgtggctgc gagggcaggg cataacgcac
    14461 ctgccattta acaaggaaaa aacatgaaaa cgttaattgc ccggcataaa gctggtgaac
    14521 atatcggcat atgttcagtc tgttctgccc atccgttggt tatcgaagcg gcgctggcat
    14581 ttgatcgcaa cagcacgcgc aaagtgctga ttgaagcaac gtcaaaccag gtcaatcaat
    14641 ttggcggtta taccggaatg acaccggcag actttcgcga atttgttttt acgattgccg
    14701 ataaagttgg gtttgcacgc gaacgcatta ttctcggcgg cgatcatctg ggaccaaact
    14761 gctggcagca agaaaatgcg gatgcggcga tggaaaaatc cgtcgagctg gtaaaagcat
    14821 atgttcgtgc tggcttcagt aaaattcatc ttgatgcgtc aatgtcctgc gcgggggata
    14881 ccataccgtt agcaccagaa acggttgcgg aacgagctgc tgtgctttgc tttgctgcgg
    14941 aaagtgtggc gacagattgc cagcgtgagc aactgagcta tgtcattggc actgaagttc
    15001 cggttccggg cggtgaggcc agcgccattc agtcagtaca catcacccat gttgaagatg
    15061 ccgccaatac tttacgtacg catcaaaagg cctttattgc ccgtgggctg acagaggcgt
    15121 taacacgtgt gattgccatc gtggtgcagc cgggtgtgga atttgatcac agcaatatta
    15181 tccattatca gccgcaggaa gcgcaggcgc tggcgcaatg gatagaaaac acccgaatgg
    15241 tttatgaagc acattctacc gattaccaga cccggacggc ttattgggaa ttagtccgcg
    15301 atcactttgc aatattgaaa gtcggtcccg cattaacctt tgctttacgc gaggcgatat
    15361 ttgcactggc acaaattgag caggaactta tcgcccccga aaatcgcagc ggttgcctgg
    15421 cggtaattga agaagtgatg ctcgacgaac cgcaatactg gaaaaaatat tatcgcacgg
    15481 gttttaacga ttcattactg gatattcgtt acagcctgtc ggatcgtatt cgttattact
    15541 ggccgcatag tcggattaaa aatagcgttg aaacgatgat ggtgaatctg caaggcgtgg
    15601 acatcccact gggcatgatt agtcagtatc ttcccaaaca atttgaacgc attcagtccg
    15661 gggaattatc agcaatgccg catcagctta ttatgaataa aatttatgat gttttgcgcg
    15721 cctatcgcta cggctgtgcg gaataaggac ggtatatgac taacctgttt gttcgtagcg
    15781 gaatttcttt tgtcgatcgt agcgaagttt taacctatat cggtaatgag atgctcgcga
    15841 aaggtgtggt tcatgatacc tggccacagg cattaattgc cagagaagca gaattcccta
    15901 ccgggataat gcttgagcag cacgccattg caataccgca ttgtgaggcg attcatgcta
    15961 agtcgtcagc catttatctg ttaaggccaa caaataaagt tcattttcag caagcggatg
    16021 atgataacga cgtggcggta tcgttggtta ttgcgttgat tgtggaaaat ccgcagcagc
    16081 aattgaaact tttacgctgt ttatttggca agttacaaca gcccgatatc gtcgagacac
    16141 taattactct tcctgaaact cagttaaagg aatacttcac aaagtatgtt ttagattcag
    16201 acgaataaat ccctctgtaa caataaaaag gactatttat gaaacgcaag attattgtcg
    16261 cttgcggagg cgcggttgcg acctctacga tggcggcgga agaaattaaa gagttgtgtc
    16321 agagtcataa tattcctgtt gaattaatcc agtgtcgggt taatgaaata gaaacctata
    16381 tggatggtgt gcatttgata tgtaccactg ccagggtgga tcgtagtttt ggcgatattc
    16441 cgttagttca cggcatgcct tttgtttctg gtgtcggtat cgaagcatta caaaataaaa
    16501 ttctgactat cttacaggga tgacctatgt tttcagaagt catgcgttat attctcgacc
    16561 tcggccctac agtgatgctg ccggttgtca tcattatttt ttctaaaata ttaggcatga
    16621 aggcaggcga ttgctttaaa gcgggtctgc atatcgggat tggctttgtt ggcattggcc
    16681 ttgtgattgg cttaatgctg gattccattg gtccggcggc gaaagcgatg gcggaaaatt
    16741 tcgacctgaa tctgcatgtg gtcgatgtcg gctggccggg ctcttcacca atgacctggg
    16801 cgtcgcaaat tgcgctggtg gcgattccga ttgcgatatt ggttaacgtg gcgatgttac
    16861 tgacccgtat gacgcgggtg gtaaatgttg atatctggaa tatctggcat atgaccttca
    16921 ccggcgcgtt gctgcacctg gcaaccggtt catggatgat agggatggcg ggtgtggtaa
    16981 ttcacgcggc gtttgtttat aagctcggcg actggtttgc ccgcgatacc cgaaatttct
    17041 ttgagctgga aggtattgct attccgcacg gtacgtcggc gtatatgggg ccgattgcgg
    17101 tgctggtcga tgctatcatc gagaaaatcc caggcgttaa ccgaattaaa tttagcgccg
    17161 acgatattca gcgcaaattt ggcccttttg gcgaacctgt caccgtgggt tttgtgatgg
    17221 ggctgattat cggcatcctc gcgggttacg atgtcaaagg tgtattgcag ctggcggtaa
    17281 aaacggcggc agttatgctg ctgatgccac ggatgattaa acccatcatg gatggtttaa
    17341 cgcccatcgc taagcaggcc cgtagtcgtt tacaggcgaa gttcggcggt caggagttcc
    17401 tgattggcct ggatcccgca ttactgctgg ggcatacggc ggtggtatcg gcaagcctga
    17461 tttttattcc gctcaccatt ttaattgctg tttgtgttcc gggtaatcag gtactgccgt
    17521 ttggcgatct tgccactatc ggcttcttcg tggcgatggc ggtcgcggtg catcgtggaa
    17581 atctgttccg caccttaatc tcgggtgtca tcattatgag catcaccctg tggatcgcga
    17641 cgcaaactat tggtttgcac acccaaccgg cggctaatgc tggggcgtta aaagccgggg
    17701 gtatggtggc ttcaatggat cagggcggtt ctcccattac ctggttactg attcaggttt
    17761 tctccccgca aaatattccc ggtttcatta ttatcggcgc aatttatctg accggtattt
    17821 tcatgacctg gcgtagagcg cgtggtttta ctaaacaaga gaaagccgtt ctcgcagaat
    17881 aatttttacc tgagggggag gttatccccc ttaacaa
//
LOCUS       DAZPCS010000073        17708 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZPCS010000073 DAZPCS010000000
VERSION     DAZPCS010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17708
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.073"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            104..787
                     /locus_tag="ACHUQ7_002364"
     CDS             104..787
                     /locus_tag="ACHUQ7_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFR1135743.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IVLLEKQQAQLKSQLETIQKQQTGIINSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            831..5493
                     /locus_tag="ACHUQ7_002365"
                     /pseudo
     CDS             831..5493
                     /locus_tag="ACHUQ7_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
     gene            5861..7516
                     /gene="lldP"
                     /locus_tag="ACHUQ7_002366"
     CDS             5861..7516
                     /gene="lldP"
                     /locus_tag="ACHUQ7_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFR1135744.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLNKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7516..8292
                     /gene="lldR"
                     /locus_tag="ACHUQ7_002367"
     CDS             7516..8292
                     /gene="lldR"
                     /locus_tag="ACHUQ7_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFR1135745.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8289..9479
                     /gene="lldD"
                     /locus_tag="ACHUQ7_002368"
     CDS             8289..9479
                     /gene="lldD"
                     /locus_tag="ACHUQ7_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFR1135746.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            9675..10148
                     /gene="trmL"
                     /locus_tag="ACHUQ7_002369"
     CDS             9675..10148
                     /gene="trmL"
                     /locus_tag="ACHUQ7_002369"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFR1135747.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10201..11022)
                     /gene="cysE"
                     /locus_tag="ACHUQ7_002370"
     CDS             complement(10201..11022)
                     /gene="cysE"
                     /locus_tag="ACHUQ7_002370"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFR1135748.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGCGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11102..12121)
                     /gene="gpsA"
                     /locus_tag="ACHUQ7_002371"
     CDS             complement(11102..12121)
                     /gene="gpsA"
                     /locus_tag="ACHUQ7_002371"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFR1135749.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12121..12588)
                     /gene="secB"
                     /locus_tag="ACHUQ7_002372"
     CDS             complement(12121..12588)
                     /gene="secB"
                     /locus_tag="ACHUQ7_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFR1135750.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12651..12902)
                     /gene="grxC"
                     /locus_tag="ACHUQ7_002373"
     CDS             complement(12651..12902)
                     /gene="grxC"
                     /locus_tag="ACHUQ7_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFR1135751.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGCTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13044..13475)
                     /locus_tag="ACHUQ7_002374"
     CDS             complement(13044..13475)
                     /locus_tag="ACHUQ7_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFR1135752.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKSVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13720..15264
                     /gene="gpmM"
                     /locus_tag="ACHUQ7_002375"
                     /gene_synonym="pgmI"
     CDS             13720..15264
                     /gene="gpmM"
                     /locus_tag="ACHUQ7_002375"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFR1135753.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15298..16557
                     /gene="envC"
                     /locus_tag="ACHUQ7_002376"
     CDS             15298..16557
                     /gene="envC"
                     /locus_tag="ACHUQ7_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFR1135754.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVTMQHAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16561..17520
                     /locus_tag="ACHUQ7_002377"
     CDS             16561..17520
                     /locus_tag="ACHUQ7_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFR1135755.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(17507..17708)
                     /locus_tag="ACHUQ7_002378"
                     /pseudo
     CDS             complement(17507..17708)
                     /locus_tag="ACHUQ7_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT         4423 a         4609 c         4639 g         4037 t
ORIGIN      
        1 acacacatca ctgtcattaa tgtaaagcaa catagctcac caataataca atggacaact
       61 ctaccggcat tgagcgctgg tcggaggaaa taaggatttt tacatgaact tgaagaagat
      121 ttttttcagt gctgtcactg tttcagttct ctgcgcatta acgggatgtg actacattga
      181 agaaggcaag ccagaaagta gtttactgaa acaacaagaa gaacataata ataaaattgt
      241 gctacttgaa aaacaacaag cacagctaaa gagtcaactc gaaactattc aaaaacaaca
      301 aactggcata ataaacagta caaagacttt aactcacgta attaaatccg ttaaagacca
      361 acaaaatact tttattttta ccgagtttaa cccagcaaaa acgaagtatt tcatcctgaa
      421 caacggttcg gtggctttag cgggtcgagt gttatccatt gacgccacag agaatggtag
      481 tgttattcat atctcactgg tcaacttatt aagtacacct atctcaaaca ttggttttaa
      541 tgcgacatgg ggtggtgaaa aacctgtcga tgccaaagag tttgcccggt ggcaacaatt
      601 acttttcaac acatcaatga aatccacatt gaaattatta ccaggtcaat ggcaagacat
      661 taatttgacc ctgaagggtg tatcgcccaa taacctggga tatctgaagt tagccatcaa
      721 catggaaaat attcagttcg acaatcttcc ctctgctgaa aatcggcaga aaagaagcaa
      781 aaaataaatg cagttagtac gcttataaca aggaatagcg agcatcatta atgaacaaaa
      841 tatttaaagt tatctggaac cctgcgacag ggaattatac tgttaccagc gaaacggcaa
      901 aaagccgtgg caagaaatct gggcgcagta agctgttaat ttctgcgctg gttgcgggtg
      961 gaatgttgtc gtcgtttggg gcattggcga atgccgggaa tgacaacggt cagggtgttg
     1021 attacggtag tggatcagct ggcgacggct gggttgctat aggcaaaggg gcgaaagcaa
     1081 atacttttat gaacaccagt ggttccagta ctgctgtggg ttatgacgct atagctgaag
     1141 gccaatatag ctctgccatc gggtcaaaaa cccatgcgat tggtggtgca tcaatggcct
     1201 ttggggttag tgcaatatca gaaggcgata gaagtatagc actgggtgcc tcttcgtatt
     1261 cattgggcca atactcaatg gccctcggcc gttattcaaa agcattgggt aaattgtcta
     1321 ttgctatggg ggactcttcc aaagcggaag gagcaaacgc cattgccctg gggcgtagca
     1381 gtgtagctag cggtactgac agcctcgcat ttggcagaca atcacttgcc agcgcagcga
     1441 acgctattgc gataggtgct gagaccgaag ccgctgaaaa tgcaactgct attggcaata
     1501 atgcgaaggc aaaagggact aatagcatgg caatggggtt cggaagcctt gccgataaag
     1561 tcaatactat cgcattagga aatggcagcc aggctctggc agataatgca atcgccatag
     1621 gccagggcaa caaagctgat ggcgtggatg ccatcgctct gggtaatggt agccagtcga
     1681 gaggcttaaa caccattgcc ttaggcacag ccagtaatgc aactggtgat aagagtcttg
     1741 cgcttggtag taatagcagt gccaacggta ttaactctgt cgcgctgggc gcagattcca
     1801 ttgcggattt agacaatacc gtctctgtcg gcaatagttc attaaaacgc aagatcgtta
     1861 atgtgaaaaa tggcgcgatc aagtctgaca gttacgatgc cattaatggt tcacagcttt
     1921 atgccattag cgactcggta gcaaaaaagg cttggaggag gggctgcagt agatgttgat
     1981 gacggtactg ttacagcacc aacctacaat ttaaaaaatg gtagcaaaaa taacgtaggg
     2041 gctgcgctcg ctgtacttga tgaaaacacc ctgcaatggg accaaaccaa aggcaaatac
     2101 agcgctgctc atggtactag tagcccaact gccagcgtaa tcaccgatgt tgcggatggc
     2161 acgatttcag cctccagtaa ggatgcggtt aacggttccc aactgaaagc taccaatgac
     2221 gatgtcgaag ccaacaccac caatatcacc aacctgacgg attccgttgg tgaccttcag
     2281 gctgatgccc tgctctggaa cgaaactaaa aaggcattca gtgcagctca cggccaggat
     2341 accaccagca aaatcaccaa cgttaaagat gccgacctga cggctgacag cactgatgct
     2401 gttaacggct ctcagctgaa aaccaccaac gatgctgtgg cgacgaatac caccaatatc
     2461 gccaataaca cttccaatat tgccactaac accaccaaca tctctaacct gactgagacg
     2521 gtgactaatc ttggtgagga tgcgctgaaa tgggataagg acaatggtgt attcacggca
     2581 gctcatggca ccgagaccac cagcaaaata accaacgtta aagatggcga cctgacgact
     2641 ggcagcaccg atgccgttaa cggctctcag ctgaaaacca ccaacgatgc cgtggcgacg
     2701 aataccacca atatcgccac taacaccacc aacatctcta atctgactga gacggtgact
     2761 aatcttggtg aggatgcgct gaaatgggat aaggacaatg gtgtcttcac tgcagctcat
     2821 ggcaacaata ccgccagcaa aatcaccaat atcctggacg gcacagtcac tgcaaccagt
     2881 tccgatgcca ttaacggtag ccagctttat gacttaagca gcaatatcgc cacctacttc
     2941 ggcggcaatg cttctgtgaa tactgacggt gtgtttaccg gtccaaccta caaaatcggt
     3001 gaaacaaatt attataacgt cggcgatgca ctggctgcga ttaactcctc atttagcacg
     3061 tctctcggcg atgctctgct ttgggatgcc accgcaggta aattcagtgc caaacacggt
     3121 actaatggtg acgcaagcgt gatcactgat gtcgcagatg gtgaaatttc agactccagt
     3181 tctgacgcag taaacggctc acaactccac ggcgtgagca gttatgttgt tgatgcgctg
     3241 gggggtggtg ccgaagtcaa tgcagacggc accatcactg cgccgacgta caccattgct
     3301 aatgctgatt acgataatgt cggtgatgcc ctgaatgcta tcgataccac tcctgacgac
     3361 gctctgctct gggatgcgga cgccggtgaa aatggtgcat ttagcgccgc tcacggaaaa
     3421 gataaaactg ccagtgtaat cactaacgtc gctaacggtg caatctctgc tgccagcagc
     3481 gacgcgatta acggctcaca actctatacc accaataagt acatcgctga tgcgctgggt
     3541 ggtgacgcag aagtcaacgc tgacggcacc atcaccgcac cgacttacac cattgcgaac
     3601 gccgagtaca acaacgtcgg tgacgccctg gatgcgcttg atgataacgc cctgctgtgg
     3661 gataagactg ccaatggcgg tgctggagcc tacaatgcca gccatgacgg taaagccagc
     3721 atcatcacta atgtcgctaa tggcagtatt agtgaggaca gtaccgatgc agtgaacggt
     3781 tctcagttga atgcgacgaa tatgatgatt gagcagaaca cccaaattat caatcagctc
     3841 gctggtaaca ccgacgcaac ctatatcgaa gaaaatggcg ctggtatcaa ctacgttcgt
     3901 actaacgaca acgatttagc ctttaacgat gcaagcgcct ctggtgtcgg cgctacagct
     3961 gtaggttata acgctgtcgc gtctggtgcc agcagcgtag ccattggtca gaacagcagc
     4021 agcaccgttg ataccggtat tgcgctgggt agcagctccg tttccagccg tgtgattgcc
     4081 aaaggttctc gtgacactag cgtaacggaa aatggcgtgg tgattggtta tgacaccact
     4141 gacggcgaac tgctaggtgc attgtcaatt ggtgatgacg gtaaataccg ccaaatcatc
     4201 aacgtagctg atggttcaga agcccatgac gccgttacgg ttcgccagtt gcagaacgct
     4261 attggagcgg tcgccactac gccaaccaag tactttcacg ccaactcaac ggcagaagac
     4321 tcactggccg ttggtgaaga ctcgctggca atgggtgcga aaactgtcgt taatggtaat
     4381 gcaggtattg gtatcggttt gaacactctg gttctggctg atgcgatcaa cggcattgct
     4441 atcggcagta acgcacgcgc aaaccatgca aacagtatcg caatgggtaa cggttctcag
     4501 accacccgtg gtgcacagac tggctacacc gcctacaaca tggacgcacc gcagaactct
     4561 gtaggtgagt tttctgtcgg tagtgaagac ggtcaacgtc agatcacaaa cgtcgcagct
     4621 ggttcagcgg ataccgatgc ggttaacgtg ggtcagttga aagtcactga tgagcgcgta
     4681 gcgcaaaata cccagagcat tactaacctg aacaatcagg tcactaatct ggatactcgc
     4741 gttactaata tcgaaaacgg tattggcgac attgtcacca ccggtagcac caagtacttc
     4801 aagaccaaca ccgatggcgt agatgccaac gcccagggta aagatagcgt tgctattggt
     4861 tctggttcca ttgctgccgc tgacaacagc gtcgcactgg gtaccggttc cgttgcagag
     4921 gaagaaaata caatctctgt aggttcttct actaaccaac gccgtatcac caacgtagct
     4981 gccagtgtta atgccaccga tgcggttaac gtgtcgcagc tgaaatcttc tgaagcaggc
     5041 ggagtgcgct acgacaccaa agctgatggt tctatcgact atagcaatat caccctcggt
     5101 ggtggcaacg gcagtacgac tcgtatcagc aacgtctccg ctggcgtcaa caacaacgac
     5161 gcggtgaact acgcgcagtt gaagcaaagc gcgcaggaaa cgaagcaata caccgatcag
     5221 cggatggttg agatggataa caaactgtct aaaactgaaa gcaagttgag cggtggtatc
     5281 gcttctgcaa tggcaatgac cggtctgccg caggcttata caccgggtgc cagcatggct
     5341 tctattggtg gcggtactta caacggtgaa tcggcagttg ctttaggtgt atcgatggtg
     5401 agcgccaatg gtcgttgggt ctacaaatta caaggtagta ccaatagcca gggtgaatac
     5461 tccgccgcac tcggtgccgg tattcagtgg taatgatcca ttaagttagt gtgactaagg
     5521 gcaaacgcca aattgcctga tgcgttacgc gcatcaggca agataaggcg ctccgggtca
     5581 acaatctaag ggcaattctc tgatgaggat tgcccttttc tttaccagac atctcccccc
     5641 acaagaattg gccctaccaa ttcttcgctt atctgacctc tggttcacaa ttccccaatt
     5701 aaaactcaca tcaatattgc caatacataa catttagtta accattcatt gtcattatcc
     5761 ctacacaaca caattggcag tgccactttt acacaacgtg tgacaaggag atgagcaaca
     5821 gactcattac acgatgtgcg tggactctag gagacctgca atgaatctct ggcaacaaaa
     5881 ctacgatccc gccgggaata tctggctttc cagtctgata gcatcgcttc ccatcctgtt
     5941 tttcttcttt gcgctgatta agctcaaact gaaaggatac gtcgccgcct catggacggt
     6001 agcaatcgcc cttgccgtgg ctttgctgtt ctataaaatg ccggtcgcta acgcgctggc
     6061 ctcggtggtt tatggcttct tctatggttt gtggcccatc gcgtggatca ttattgcagc
     6121 agtgttcgtc tataagatct cggtgaaaac cgggcagttt gacatcattc gctcgtctat
     6181 tctttcgata acccctgacc agcgtctgca aatgctgatc gtcggtttct gtttcggcgc
     6241 tttccttgaa ggagccgcag gctttggcgc accggtagca attaccgccg cattgctggt
     6301 cggcctgggt tttaaaccgc tgtacgccgc cggactgtgc ctgattgtta acaccgcgcc
     6361 agtggcattt ggcgcgatgg gcattccaat cctggttgcc ggacaagtaa caggcatcga
     6421 cagctttgag attggccaga tggtggggcg tcagctaccg tttatgacca ttatcgtgct
     6481 gttctggatc atggcgatta tggacggctg gcgcggtatc aaagagacgt ggcctgcggt
     6541 cgtggttgca ggcggctcgt ttgccatcgc tcagtacctt agctctaact tcattgggcc
     6601 ggagctgccg gacattatct cttcgctggt atcactgctc tgcctgacgc tgttcctcaa
     6661 acgctggcaa ccagttcgcg tattccgctt cggtgacttg ggggcgtcac aggttgatat
     6721 gacgctggcc cacaccggtt acaccgcggg tcaggtgtta cgtgcctgga caccgttcct
     6781 gttcctgacc gctaccgtaa cactgtggag catcccgccg tttaaagccc tgttcgcatc
     6841 gggtggtgcg ctgtatgagt gggtgatcaa cattccggtg ccgtacctca ataaactggt
     6901 tgcccgtatg ccaccagtgg tcagcgaggc tacagcctat gccgccgtgt ttaagtttga
     6961 ctggttctct gccaccggca ccgccattct gtttgctgca ctgctctcga ttgtctggct
     7021 gaagatgaaa ccgtctgacg ctatcagcac cttcggcagc acgctgaaag aactggctct
     7081 acccatctac tccatcggca tggtgctggc gttcgccttt atctcgaact attccggact
     7141 gtcatcaaca ctggcactgg cactggcgca caccggtcat gcattcacct tcttctcgcc
     7201 gttcctcggc tggctggggg tattcctgac cgggtcggat acctcatcta acgccctgtt
     7261 cgccgcgctg caagccaccg cagcacaaca aattggcgtc tctgatctgt tgctggttgc
     7321 cgccaatacc accggtggcg tcaccggtaa gatgatctcc ccgcaatcta tcgctatcgc
     7381 ctgtgcggcg gtaggcctgg tgggcaaaga gtctgatttg ttccgcttta ctgtcaaaca
     7441 cagcctgatc ttcacctgta tggtgggcgt gatcaccacg cttcaggctt atgtcttaac
     7501 gtggatgatt ccttaatgat tgttttaccc agacgcctgt cagacgaggt tgccgatcgt
     7561 gtgcgggcgc tgattgatga aaaaaacctg gaagcgggca tgaagttgcc cgctgagcgc
     7621 caactggcga tgcaactcgg cgtatcacgt aattcactac gcgaggcgct ggcaaaactg
     7681 gtgagcgaag gcgtgctgct cagtcgacgc ggcggcggga cgtttattcg ctggcgtcat
     7741 gacacatggt cggagcaaaa catcgtccag ccgctaaaaa cgctgatggc cgatgatccg
     7801 gattacagtt tcgatattct ggaagcccgc tacgccattg aagccagcac cgcatggcat
     7861 gcggcaatgc gcgccacacc tggcgacaaa gaaaagattc agctttgctt tgaagcaacg
     7921 ctaagtgaag acccggatat cgcctcacaa gcggacgttc gttttcatct ggcgattgcc
     7981 gaagcctcac ataacatcgt gctgctgcaa accatgcgcg gtttcttcga tgtcctgcaa
     8041 tcctcagtga agcatagccg tcagcggatg tatctggtgc caccggtttt ttcacaactg
     8101 accgaacaac atcaggctgt cattgacgcc atttttgctg gtgatgccga cggggcgcgt
     8161 aaagcaatga tggcgcacct tagttttgtt cacaccacca tgaaacgatt cgatgaagat
     8221 caggctcgcc acgcacggat tacccgcctg cccggtgagc ataatgagca ttcgagggag
     8281 aaaaacgcat gattatttcc gcagccagcg attatcgcgc cgcagcgcaa cgcattctgc
     8341 cgccgttcct gttccactat atggatggtg gtgcatattc tgaatacacg ctgcgccgca
     8401 acgtggaaga tttgtcagaa gtggcactgc gccagcgtat tctgaaaaac atgtccgact
     8461 taagcctgga aacgacgctg tttaatgaga aattgtcgat gccggtagca ctggcaccgg
     8521 tgggtttgtg tggcatgtat gcgcgtcgtg gcgaagttca ggcagccaaa gcggcggacg
     8581 cgcatggcat tccgtttact ctctcgacgg tttccgtttg cccgattgaa gaagtcgcgc
     8641 cagccatcaa gcgcccaatg tggttccagc tttatgtact gcgcgatcgc ggctttatgc
     8701 gtaacgcgct ggagcgagca aaagcagcgg gttgttcgac gctggttttc accgtggata
     8761 tgccgacacc gggcgcacgc taccgtgatg cgcattcagg tatgagcggc ccaaatgcgg
     8821 caatgcgccg ctacttgcag gcggtgacgc atccgcaatg ggcgtgggat gtgggcctga
     8881 acggtcgtcc acatgattta ggtaatatct cagcttatct cggcaaaccg accggactgg
     8941 aagattacat cggctggctg gggaataact tcgatccgtc catctcatgg aaagaccttg
     9001 agtggatccg cgatttctgg gatggcccga tggtgatcaa agggatcctc gatccggaag
     9061 atgcgcgcga tgcagtacgt tttggtgctg atggaattgt ggtttctaac cacggtggcc
     9121 gccagctgga cggtgtactc tcttccgctc gtgcgttgcc cgctattgca gatgcggtga
     9181 aaggtgatat cgccattctg gcggatagcg gaattcgtaa cgggcttgat gtcgtgcgta
     9241 tgattgcgct cggtgccgac accgtactgc tgggtcgtgc tttcctgtat gcgctggcaa
     9301 cagcgggcca ggcgggtgta gctaacctgc taaatctgat cgaaaaagag atgaaagtgg
     9361 cgatgacgct gactggcgcg aaatcgatca gtgaaattac gcaagattcg ctggtgcagg
     9421 ggctgggtaa agagttgcct acggcactgg ctccgatggc gaaagggaat gcggcatagt
     9481 cgtttgcgcc ccctcaccct aaccctctcc ctcagggaga ggggaccgtt cggcgctgta
     9541 tgtactccct cactctgaaa cgacaccgca ctcttttttt ctccctcgcc cctccgggga
     9601 gggccggggt gaggggaaaa ggccgcacgg ttcaccaatt ttctgctatc ctgcccccgc
     9661 actaaggggg cagtatgcta aacatcgtac tttacgaacc agaaattccg ccaaatactg
     9721 gcaacatcat ccgtctttgc gctaataccg gctttcgtct acatatcatc gaaccgatgg
     9781 gatttgcctg ggacgacaag cgcctgcgcc gcgcggggct ggactatcac gagtttaccg
     9841 ccgttacgcg tcatcatgac tatcgcgcgt tcctcgaagc agaaaatccc cagcgcctgt
     9901 ttgccctcac cacgaaaggt acgcctgctc acagcgccgt aagctatcag gatggcgatt
     9961 atctgatgtt cggcccggaa acacgcggcc tgccagcgag cattcttgat gccctgcccg
    10021 ctgaacaaaa aattcgcatt ccgatggtgc cggacagccg cagcatgaat ctgtccaatg
    10081 cggtgtcggt agtggtgtat gaagcctggc ggcagctggg atatccggga gcgttgttga
    10141 gagattagtt actgtatgcc ggatagatga ttacatcgca tccggcacga tcacaggaca
    10201 ttagatccca tccccatact caaatgtatg gttaataccg ttgaaatgct ggtccatatc
    10261 cattgatggc ttatcgctgt ctggtttacc gacaatacga gccggaacgc cagcggcggt
    10321 tgtatgcggc ggcaccggtt gcagcaccac ggaacctgcg ccaatcttcg cgccgcaacc
    10381 aacttcaata ttgccgagga ttttcgcgcc cgcgccaatc atcacacctt cacgaatttt
    10441 cgggtgacgg tcaccaccag atttacccgt accgccaagc gtcacagatt gcagaatcga
    10501 tacgtcgttt tcaattaccg ccgtttcacc cacaacgatg cccgtggcgt ggtcgagcat
    10561 aatgccacgg ccaatttttg ctgccgggtg aatatcgacc tggaacgtca cagaaacctg
    10621 gttttgcaga aagattgcca atgcgcgacg cccctgattc cacaaccagt gaccgatgcg
    10681 ataggcctgc aaggcatgaa aacccttcag gtataacaac ggggttgagt atttatcgac
    10741 tgccgggtcg cgggtacgca ccgcctgaat atcacaggcc gcagaggcga tcatttccgg
    10801 gtcagcggcg taggcttctt caaccacttc acggatagca atagcaggca taattggcga
    10861 tgacagcttg ttcgccagca tgtagctcag tgcactgcca aggttttcgt gcttgagtag
    10921 cgtcgcgtgg taaaaactgg ccagcattgg ctcacagtcc gccagcgttc tggcttcggc
    10981 tttaatattg ttccagacaa tttccagttc ttcacacgac attgcttact ccacacgatg
    11041 agataatgac cggcccgttc tgcgcgggcc gggtcatagc ggtaacaaag gttccctggg
    11101 gttagtgact gctgcgctcg tccttgcgtg cacgacctaa taatgtcaat gctgcctcgc
    11161 gcgcgttttt tccgcaatat aatacttgat aaatttcctc ggttattggc atttcaacgc
    11221 cgaagcgatg cgccagttcg cggacttctt tcgtattgcg gtagccttcc accacctgac
    11281 caatcttctc ctgcgcgctt tgtacatcca tgccctgacc gagcatcata ccaaaacggc
    11341 ggttacgcga ctggttgtcg gtacaggtaa gcaccagatc gcccagcccc gccatgccca
    11401 taaaggtggc aggatcggca cccagcgccg caccaagacg cgacatttca gccagcccac
    11461 gggtgatcag cgccgtacgc gcattcgcac caaaaccgat accgtcagac atccccgcgc
    11521 caatggcaat gacgtttttc accgcgccgc caagctgcac gccaatgaaa tccgggttgc
    11581 tgtaaacgcg gaaacttttg ccgcagtgca gcagctgctg gagatcatcg gcaaaggtct
    11641 gatcggtcga ggccagcgaa atagctgtcg gtaaacctgc cgccagttct ttcgcaaacg
    11701 ttgggccaga gataaccgcc agcggaattt gatcgcctaa ggcctcacgc gccacgtcct
    11761 gtaacagacg tccggtttcc gcttccagcc ctttggtcgc ccacaccaga cgcgcatcag
    11821 gacgcatcag cggtttaatc tggcgcagca cttcaccaaa gacatggctg ggtacgacga
    11881 cgagaatatt acgactggct gccagcgcag tggcgagatc gctttcaaga tggagcgtat
    11941 cgggaaaagg cacatcgggg agaaacgcgg cgttacagcg gtcgcgttca agcgttgcga
    12001 tatgttcagg gtcatggccc cagaggacaa cctcgtggcc atttcttgcc agggtaatgg
    12061 caagagcggt gccgtacgag ccggcaccga tcacagtcat tgaagcatta cgttggttca
    12121 tcaggcatcc tgatgttctt cagtaccttc gccagcctgc tgctgcaaat agttcatgaa
    12181 cagcgcatcg aagttaaccg gcgcaaggtt cagttgcggg aatgtaccgc gggataccat
    12241 gctggtgatg cactcacgag catacgggaa cagaatgttc gggcagtatg cgcccaggca
    12301 atgtgccatc tgggtgcctt cgatacccgc gatggagaaa ataccgccct gctgaacttc
    12361 acacaggaac gcggtttctt cgcccaagga ggccgttacg gtaacacgca gtaccacttc
    12421 gtatacgtca tctgccagtt gggaagatgc cgtatccaga tcaagtttaa cttctggttg
    12481 ccaatctttc tggaaaacgt gcggcgcgtt cggcgcttcg aaagagatat ccttggtata
    12541 aatacgttgg atctggaaag tcatttcagt gttgttttgt tctgacatgt gtagaaaacc
    12601 cttaagtgtt gtccttaaat actgcgcaat gtaataccaa cgttcacacg ttatttcagc
    12661 aggggatcca gtccaccacg tgcatccaat gcatacaagt catcacagcc gccaatgtgc
    12721 tgtgcgtcaa taaaaatctg gggaaccgtg gtgcaaccgc tgcgtttgat catctcttca
    12781 cgcttggcgg cattgccatc gatcggcaac tcctggaaac tcacgccctt gctgctcagc
    12841 agtgcttttg cacgatggca atacgggcag gtttctttgg tatagatttc aacattggcc
    12901 ataatttagc tcctgttttt ttaccctgaa atttcatgtt gcagggcggc tgcaaaatgg
    12961 agtgtcccca gaagcttata acaataagtc gctgggggcc taattgcagc taacggcctg
    13021 catcatgaaa gacgacaggt aaattatttg ccgcgcacca gaggcaagtt ttcgccagcc
    13081 cagccagcaa cgccctcttt cagcacgaat acttgcgcaa aaccagcttt cgtcagcgcg
    13141 tttgcaggct cctggcactg catgccagaa ccgtctacca cgataaccga tttgtctttg
    13201 tgcttctcaa gctcaccaac attgttggct ttgatttcgc tcggcaacag attgatagaa
    13261 cctgcgatat ggcctttacg gaagtcatca cgctgacgta aatccacaac cacagcgtct
    13321 tctttgttga tcagacgcgt agcttcacca cgagtaatca ccttcacttt cgaggtcagg
    13381 ctcttaaacg tagtcacaag aaccgccacc agtaacgcga tccaggcgat actcagtatg
    13441 ggatgacggc caacaaattg cataatttct tgcatggggg gtaacaactc ccgacgtagt
    13501 gattaaaaaa accaggaaag gagtatacct gcgcggtgcg gcaaatacag ccagcgcgtt
    13561 aactggaatg caattttgcg gggcacgacg aaaaaaaagc gcaaatctgg catcggcttg
    13621 ccgcgagcgg tcgtatattt tgatcttcag aggctatttt atcgattcag ctgtagtaaa
    13681 attacgcaaa ttttgactct tgagtatgag gttgtcgcaa tgtcggtttc taaaaaacct
    13741 atggtactgg tgattctgga tggctatggc tatcgtgaag aacagcagga taacgccatt
    13801 tttagtgcta aaaccccggt aatggatgca ctgtgggcca atcgtccgca taccctgatc
    13861 gacgcttccg gtctggaagt cggtctgcct gaccgtcaga tgggtaactc cgaagtaggc
    13921 cacgttaacc tgggtgccgg ccgcatcgtg tatcaggacc tgactcgtct ggacgttgaa
    13981 atcaaagatc gcgctttctt tgctaatcca gtgctggctg gcgcggtaca taaagcgaaa
    14041 aacgcaggta aagcagtaca cattatgggt ctgctctccg caggcggcgt acacagccac
    14101 gaagatcaca tcatggcgat ggtagaactg gcagctgaac gcggcgcaga aaaaatctac
    14161 ctgcacgcat tccttgacgg tcgcgacacc ccgccgcgca gtgctgaatc ctcgctgaaa
    14221 aaatttgaag agaaatttgc tgcgctgggt aaaggccgcg tagcgtccat cattggtcgt
    14281 tactacgcga tggaccgcga taaccgttgg gatcgcgttg aaaaagccta tgacctgctg
    14341 actctggcgc agggtgagtt ccaggccgat actgccgttg ctggtttgca ggcagcttat
    14401 gctcgcgacg aaaacgacga gttcgtgaaa gcgaccgtta ttcgtgcaga aggtcagcca
    14461 gatgcggcaa tggaagacgg cgatgcgctg attttcatga acttccgtgc tgaccgcgcg
    14521 cgtgaaatca ctcgtgcatt cgtgaacgct gatttcgatg gcttcgcgcg taagaaagtg
    14581 gttaacgtcg atttcgtgat gctgaccgaa tacgccgctg acatcaaaac tgcggttgct
    14641 tacccacccg cttccctggt taacaccttc ggcgagtgga tggcgaaaaa cgacaaaact
    14701 cagttgcgta tttccgaaac cgaaaaatat gcccacgtta ctttcttctt caacggtggc
    14761 gtagaagagt cgttcaaagg cgaagatcgc attctgatca actcaccgaa agtggctacc
    14821 tacgatctgc aaccggaaat gagctccgca gagctgaccg aaaaactggt tgcggccatc
    14881 aagagcggca aatacgacac cattatctgt aactatccga acggcgacat ggtaggtcac
    14941 accggggtga tggaagcggc ggttaaagcg gttgaagcac tggatcactg cgtggaagaa
    15001 gtcgcgaaag cggttgagtc cgtgggtgga caactgctga tcaccgctga ccacggtaac
    15061 gctgaacaga tgcgcgatcc ggcaacgggt caggcacaca cggcacacac caacctgcca
    15121 gttccgctga tttacgttgg tgataagaac gtgaaagcgg ttgcaggcgg caaactttct
    15181 gacatcgcgc cgaccatgtt gtcgctgatg ggtatggaaa tcccgcaaga gatgactggt
    15241 aagccgctgt tcatcgtgga ataatccctc cccatgaggg gaaaggcgat taataccatg
    15301 acacgggccg tgaaaccgcg caggtttgca atcaggccca tcatctacgc cagcgttctt
    15361 agcgctggcg tattgttgtg cgccttttcc gcccacgcgg atgagcgtga ccaactcaaa
    15421 tctattcagg ccgatatcgc cgcaaaagag cgcgcggtac gccaaaagca acaacaacgc
    15481 gcaagcctgc tcgcacaatt gaaaaagcag gaagaagcga tctctgaagc cacccgtaag
    15541 ctgcgcgaaa cgcaaaacac gctcaatcaa ctcaataaac agattgatga gatgaacgcg
    15601 tcgattgcca aactggagca gcaaaaagcc gcccaggagc gcagcctcgc cgcacaactg
    15661 gatgccgcat tccgtcaggg cgagcatacc ggtattcagc taattctcag cggtgaagaa
    15721 agccagcgtg gacagcgttt acaggcttat ttcggctatc tcaaccaggc gcgacaagaa
    15781 accattgccc agttgaagca aacgcgtgaa gaagtcacca tgcagcatgc tgaactggaa
    15841 gagaaacaaa gcgagcaaca aacgctgtta tatgaacagc gcgcccaaca ggcgaaactg
    15901 actcaggctc tgaacgagcg taaaaagacg ctggcggggc tggaatcttc catccagcaa
    15961 ggtcagcagc agctaagtga actgcgtgcc aacgaatccc gtctgcgtaa cagcattgcc
    16021 cgtgctgaag ccgcggcgaa agcgcgtgct gaacgagaag cgcgtgaggc ccaggcggtt
    16081 cgcgagcgcc agaaagaagc gacgcgcaaa ggcaccacct acaaaccgac cgaaagcgaa
    16141 aaatcgctga tgtcccgaac tggtggcctg ggtgcgccgc gtggtcaggc attctggccg
    16201 gttcgcgggc cgacgctgca tcgctatggc gagcagctac agggtgaatt acgctggaaa
    16261 gggatggtta tcggtgcctc tgaaggtact gaagttaaag caattgccga cggtcgagtg
    16321 attctggctg actggctgca aggttacggt ctggtggtgg tggttgagca cggtaaaggc
    16381 gacatgagtc tttacggcta taatcagagc gcactggtga gcgttggttc gcaggttcgc
    16441 gcgggccagc caattgcact ggtgggcagc agtggcggtc agggtcggcc ttcactctat
    16501 ttcgaaattc gccgccaggg tcaggcggtc aatccacagc cgtggttggg aagataagtt
    16561 ttgtttccat ttcgtcgtaa cgttcttgca tttgccgctc tgttggcgct ctcctccccc
    16621 gtacttgctg gcaaacttgc catcgtcatt gatgattttg ggtatcgccc gcacaacgaa
    16681 aaccaggtgc tggcgatgcc ttccgctatc tccgtcgctg tattacccga ttcaccgcac
    16741 gccagagaga tggcgaccaa agcgcataac agcggtcacg aagtgttgat tcatctcccg
    16801 atggcaccgt tgagtaaaca gccgctggag aaaaatacgc tacgcccgga gatgagcagc
    16861 gacgaaattg agcgcattat tcgtagtgcg gtcaataacg tgccctatgc cgtggggatc
    16921 aacaaccaca tgggcagcaa gatgacctct aacctgtttg gtatgcagaa agtgatgcag
    16981 gcgctggagc gttacaatct ttacttcctc gacagcgtaa ccatcggtaa tacccaggcg
    17041 atgcgcgccg cgcaaggtac tggcgtgaag gtgatcaaac ggaaggtgtt cctcgacgat
    17101 tcgcagaacg aagcggacat ccgtgtgcaa tttaatcgcg caattgatct ggcgcgtcgc
    17161 aacggttcaa ccattgccat tgggcatcct cacccttcaa cggtacgcgt gctacaacag
    17221 atggtttata acctgccgcc agacattacg ctggtgaaag ccagcagctt gctgaatgaa
    17281 ccgcaggttg atacttctac accgccgaaa aacgctgtgc ctgatgcacc gcgtaatccg
    17341 ttccgtggcg tgaagctgtg caaaccgaag aaaccgatag aacctgttta tgctaatcgc
    17401 ttctttgaag tattaagcga aagcatcagc cagagcacgc tgatcgttta cttccagcat
    17461 cagtggcaag gctggggcaa acagcccgaa gcggcgaagt ttaacgctag cgcaaattaa
    17521 aggcgctacg cgtaatgcga tggtgcgacc gcgttttgtc gcgccattgc cataagcgga
    17581 aagaccacag taacgcctgg taaccgacct tcacgctgcg cacattggta atcaggcgct
    17641 tatacatacc cggaagtaaa tatctcggca atcatccgtt ggcgggtaag aatatccggc
    17701 tctttgcg
//
LOCUS       DAZPCS010000074        17656 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZPCS010000074 DAZPCS010000000
VERSION     DAZPCS010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17656
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.074"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(199..2834)
                     /gene="mngB"
                     /locus_tag="ACHUQ7_002379"
                     /pseudo
     CDS             complement(199..2834)
                     /gene="mngB"
                     /locus_tag="ACHUQ7_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
     gene            complement(2852..4768)
                     /gene="mngA"
                     /locus_tag="ACHUQ7_002380"
     CDS             complement(2852..4768)
                     /gene="mngA"
                     /locus_tag="ACHUQ7_002380"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HFR1135756.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LKEVYRRESLGPTALSEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPLNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQSLTLERGDETRTLQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLIRWMKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            4938..5660
                     /gene="mngR"
                     /locus_tag="ACHUQ7_002381"
     CDS             4938..5660
                     /gene="mngR"
                     /locus_tag="ACHUQ7_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HFR1135757.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(5763..6632)
                     /gene="sucD"
                     /locus_tag="ACHUQ7_002382"
     CDS             complement(5763..6632)
                     /gene="sucD"
                     /locus_tag="ACHUQ7_002382"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFR1135758.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(6632..7798)
                     /gene="sucC"
                     /locus_tag="ACHUQ7_002383"
     CDS             complement(6632..7798)
                     /gene="sucC"
                     /locus_tag="ACHUQ7_002383"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFR1135759.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(7892..9109)
                     /gene="odhB"
                     /locus_tag="ACHUQ7_002384"
     CDS             complement(7892..9109)
                     /gene="odhB"
                     /locus_tag="ACHUQ7_002384"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFR1135760.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEEPTRLLLDV"
     gene            complement(9124..11925)
                     /gene="sucA"
                     /locus_tag="ACHUQ7_002385"
     CDS             complement(9124..11925)
                     /gene="sucA"
                     /locus_tag="ACHUQ7_002385"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFR1135761.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPNSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(12226..12942)
                     /gene="sdhB"
                     /locus_tag="ACHUQ7_002386"
     CDS             complement(12226..12942)
                     /gene="sdhB"
                     /locus_tag="ACHUQ7_002386"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFR1135762.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(12958..14724)
                     /gene="sdhA"
                     /locus_tag="ACHUQ7_002387"
     CDS             complement(12958..14724)
                     /gene="sdhA"
                     /locus_tag="ACHUQ7_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFR1135763.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALHDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(14724..15071)
                     /gene="sdhD"
                     /locus_tag="ACHUQ7_002388"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(14724..15071)
                     /gene="sdhD"
                     /locus_tag="ACHUQ7_002388"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFR1135764.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(15065..15469)
                     /gene="sdhC"
                     /locus_tag="ACHUQ7_002389"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(15065..15469)
                     /gene="sdhC"
                     /locus_tag="ACHUQ7_002389"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFR1135765.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILQWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            15850..15957
                     /locus_tag="ACHUQ7_002390"
     CDS             15850..15957
                     /locus_tag="ACHUQ7_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1135766.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            16163..17446
                     /gene="gltA"
                     /locus_tag="ACHUQ7_002391"
     CDS             16163..17446
                     /gene="gltA"
                     /locus_tag="ACHUQ7_002391"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFR1135767.1"
                     /translation="MADTKAKLNLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSYGMKIARPRQLYTGYEKRDFKSDIKR"
BASE COUNT         4188 a         4786 c         4392 g         4290 t
ORIGIN      
        1 tatacgtagc ttgagagtga agataaacac aaaattacct gatgcgctac gcttgccagt
       61 gtctacatca tctctgcaat atattgaatt tgcctgcttt tgtagaccga ataaagcgtt
      121 catgccgtat ccggcatgaa caaagcgccc tttgtcaaca atctggccgc gcatatgtgg
      181 cttcggtttg attgctattc aggcaagccg gtaactgaac gtccgtgact ggccgggtaa
      241 aaaaggcccc gatagatttg aaccttgatt ttcctcggtg gtaatgtgtt catccatcat
      301 cgtttctgag caagaaatca cctcgcgact gaaagcaaca gtcgcatcac aggttgctga
      361 ttcagccgga ttaaacagcc gtaaaatcac ttcttgtcgg tcttcagctt tcttaagtgc
      421 gcttatcagg ggcatcccac tgggggcatt ttcaacaaac tataactttc cggcacgttg
      481 aatccggctt tgttgagctt catcgcatcc catgggattt tgttgtaaca ctgtactgga
      541 gtcagccatg ctcgcgcctg ctgagctaca ccagcggcgg ttggcgtacc ggtataactc
      601 aataaactta ggcgacaaga aagcagacca cgtagttgtg agtccgggac tggcatttta
      661 attcccgaag gccgcctagg ccttaaaagc agatcttctt tccccagtaa gcccacgccg
      721 cgcagcaacg taatggcaaa ggttttcttc tcttcaccga tgacttcaaa ttcacgtagc
      781 ccttcgctaa agacagccat gccgttacgc ccttcctgta aggctgcata gttgagcata
      841 ttccataccg gaaccggcgc ttctttccag ctttcttgct gccagttgtt cattgcactg
      901 tcgttcacgg ggcgcgttag cgaaccaaac tgggtatctg ccagaacact atcggtgtta
      961 aaagatgtag ggatcaggac gcgaaggcga tgatcgtcag cctggttatc aaggttgata
     1021 tccacatcaa tgcgcctgtt gttatgacta agagtgacaa ccaactcgac atctactctg
     1081 ccagtggatt gtcttgcgct gcgttgttgc agattacgcg gcaccgccat ttcatagcgg
     1141 ataacagccc tgctctgcca ggcctcatga ataatctcgc attgcggttt cgcattcgcc
     1201 gaagtaatta cccactcttc ttttgcgggt gaatagtcat attcatcacc atcatcagag
     1261 ctttcttcaa tttgcaacac ccggtcatag cgcacaccgc tatctttatc taccagttgt
     1321 agcgttccat cctcattgag cgcaatttgc cagaaagcat tttccagtat cccttcagcg
     1381 tcactttttg ccgcaatcac gttgccaggc tgattcgctt cgatataaag cgtgcgatag
     1441 cccatagaag ggacaatctg gttgatctgt atatcaaact ccataaaggg atcgtaatta
     1501 ccgtaatgaa ctatttgccg atcgattagg cctggatcga tctcacgggc atggcgaata
     1561 aaatacggta caggctgacc gcgatcgtct cgtagattaa actggctggc gcgcagccgc
     1621 acagtggtgt tgatcacttc ttcccgcggc cagggcatca ggttaaacag gacgagtttg
     1681 tcggcgtcgc tctgcggcat gttgtcggcg attttacgca tatagaaacg cagcagatta
     1741 tccgccatgt cttcagccag ttcgaagcgg gcgacgattt cgcgatgaac tttgtcacga
     1801 cagcagcagc cgatgctgtc gtgggcatga ttttttaaga tctctttcca cattttctcc
     1861 agcaagccgt ggtggtatcc aaaacccaac gtcctggcca gcgttgccag cggttccagc
     1921 agattaacaa tcttattttc aatacgtgcg tgggcaactt tgatatccat gcgcgtggaa
     1981 ccgatggtgc gatgcacgcg catatatttg ccatcaataa attccccttt cagggttgcc
     2041 agattatctc gctgcgcttc gatcttctca aatacctctt caaagcggct catcacaaac
     2101 tttcgttgag gataaatttc gcgcagtttc tccatcactt cgaagatatt ttgctgcaat
     2161 ggcatctgat catgcccatt cggcagcaaa atctctttgg ttacagacgc tttttccagc
     2221 acgtcaaaat aactgtcgag gcgtttgcgt aatccgtttt cgtccgcagg taagtactta
     2281 ccgatggcat aacccaacgg catcacctgc gccgtcactt cgctaccgtc actgctttgc
     2341 cacaaaaact cggttttatc cgtaccgtgg cgctcagaac atccacgcca gaacatggtg
     2401 caggtaatgc caaatccatt gtagatatgc ggcagttgcc cggacatgcc aaaggaatcg
     2461 ggtaaataac ctattttcat cggctcgcca aacgcgaggc agtcacgcat tccgtacatc
     2521 agattacgga cgatggattc cgcagaaaca atcgtggtat cggtctgggt ataccagggg
     2581 ccgataatca acttgccggc ttctaccagt ttcttcaagc ggtctttgtt ttccggtttc
     2641 accgcgaaat aatcttcgag gatcgccgtt tgcccgtcga gtacgtaata tttgtattcg
     2701 ttgtcctgtt ccagtcggca caggatctct tccatattat tgaccagcag aatgcgtgac
     2761 tcttcggtgg tgaaatacca ctcccgatcc cagtgcatat gcggggtgat gtgaacgcga
     2821 gataccgctt tcatcgtcgt ttcctttttg tttatggcat tacgccatca gtcagatagt
     2881 tgccatgctt aaccgcgtga cgccgccaga tcaggagaat tgcagtcgag attgcagccc
     2941 ccaccaatgc cgcaccaaac cagccaattg ccgccataac accgcccgcg ccattatcat
     3001 gaagtaaaaa gagcgagaaa atgccagcac cgggtgtcga aaggccgata ttcatcgcac
     3061 cgacaatagc gcccgttacc atagagccca gcacaaacga accaataacc cgcagcggat
     3121 cttcaatcgc catcgggatc gccccttcgg taatacctgc cagccctaac agccaggtgg
     3181 atttcccggt ctcaatttca aactctttaa acaggcgcgg tgcgagcatc gtggaagcgg
     3241 ttacggtaaa tgccgaaacc attttgacgg aggcgaaaat ggcatacggg ccgtaaacgc
     3301 cgttcgccat tgcgcccagg cagaatgcat aagcggcttt attcactggc cctccaaggt
     3361 caaaggaaca cataaaaccg agaatggcac ccagcaacag cgcgttactt cctgacagac
     3421 cgttcagcca ggcggtaagc gagttattga tccaggcgac aggttccccc accacaaaca
     3481 gcatcagact gcccgctccc aatgtaccga gcaccgggta gagataaaaa gtcaggaagc
     3541 cattgaattt actgctaaga cgcaagtgat ttttcatcca gcgtatcaag taaccggcta
     3601 tcaatccgcc aacgaccgcg ccgagaaacc cggagccgat catgttggcg gcaagtccag
     3661 ccgcaaagcc tggcgctaac gccggtttat ctgccagcga ataggcggta taggctgcca
     3721 gcaccggtac catcaaaatt ccgagcagcc cgccgcccag tttgcggtac atccacagcc
     3781 aggagttttc ttcattaaac agatcttgta gcccgaagat ttgcgacagt aataccgcga
     3841 ccgccagcac cgtccccccc gcgacaatca acggtacggc aaaagagatc ccgctcaaca
     3901 gtgcctgttt cagctccgtt ttgacgcttt tcaccggttg cgtatcttgc tgtaaggtac
     3961 gcgtctcatc gccacgctcg agggtaagcg attgttggat caacgcttct gcatggcgaa
     4021 tcggctcggc aacaggcaca gaaagcgcag gaatgccgtt aaaacgctca ctctccttga
     4081 tggcgacttc agccgcaaaa atacaggcag ttgcactatt gagttgatcc gccgttaaac
     4141 gcccttcaat accgttagca ccttgctttt caacgtagac attcacgccg agtttgcgtc
     4201 cggctttttc cagatactcc gcagccatgt aggtgtgagc aatacccgcc gggcaggccg
     4261 ttacgcaaac gatagttggc gcgttggaaa aagaggcaga aggttgcgtg cctcccttgt
     4321 catccagcgc cgagagcaat tcatcaggcg tcgtcgccga ctgtatacgc gcccgaatct
     4381 catcatccgc caggcgcgtg gtcagcgctg tcagcagttg catgtgcgta gtacccgctt
     4441 cattgagggg aatcgccagc aacaccacta aatcaaccgc ttccgggcca tccacgcctt
     4501 cccactgaag cggctcgctg agtgtggcga ctgcaaacgc cgcttctttt accgcagcag
     4561 ttttgccatg cggcacagcc aacccttcac ttaaggcggt cgggccaagg ctttcacggc
     4621 gatacacttc tttcagaaat tgctcagtac tggaaatttt ccccagagca gcaagacgtt
     4681 gagtcaacgc gtgaatggcc tcttcacggc tggtaaagcg cgcattcaga cacaacgcat
     4741 cgcggtgggt cagagtcgtc aggttcatca tgatcctcac ttgatcgttt ttatagtcgc
     4801 gccagtgtgc ccgacaaaac aataccattg cgtaatcaat ttcacttgat tgaaattatt
     4861 tgcattttat ttgtattgtt tacaggatga gtttatcggg cataatggct aaattcgtat
     4921 taatgagatg ctaaataatg ggacacaaac ccttataccg acagattgct gatcgcattc
     4981 gtgagcaaat tgcgcgtggc gagcttaaac ctggcgatgc gttacctacc gaatcggcat
     5041 tacaaacaga gtttggcgtc agccgggtta cggtgcgtca ggctttgcgc cagttagtcg
     5101 agcaacagat cctcgaaagc attcagggca gcgggactta cgtcaaagaa gagcgggtca
     5161 attacgatat ttttcagtta accagttttg atgaaaaact gtcggaccgt cacgtcgata
     5221 cacacagtga agttctgata tttgaagtga ttccggctga cgattttctt cagcaacagc
     5281 tacaaatcac cgtgcaggat cgtgtttggc atgtgaagcg cgtgcgctat cgcaagcaaa
     5341 agccaatggc gctggaagaa acctggatgc cgcttgcgtt gttcccggat ctcacctggc
     5401 aggtcatgga aaattcgaaa tatcacttta ttgaagaagt gaagaagatg gttatcgatc
     5461 gtagcgaaca ggaaatcatt cctctgatgc caaccgaaga gatgagccgt ctgcttaata
     5521 tcagccagac aaagccgatt cttgaaaaag tatcgcgtgg atatttagtt gatggtcggg
     5581 tgtttgaata tagccgcaac gcttttaata ccgatgacta taaatttacc ctgatagcgc
     5641 agcgaaaatc atcgcgataa gcacaaaaag gccatcatac gatggccaac catgtcgaaa
     5701 ccgacagccc ttcgccattc ctgtagaggg aagcggcgag ggctatttct tattacagat
     5761 atttatttca gaacagtttt cagtgcttca ccgatatccg ccaggctgcg aacggttttc
     5821 acgcctgcgg cttccagagc agcgaatttc tcatccgcag tccctttccc accggcaatg
     5881 atcgcacccg cgtggcccat acgtttgcct ttcggcgcag tcacaccagc gatgtaaccc
     5941 acaactggct tggtaacatg ctctttgatg tacgctgccg cttcttcttc agcgctaccg
     6001 ccgatctcac cgatcatcac gatcgcttcg gtctgcggat ctttttcgaa catttcgaga
     6061 atgtcgataa agttagagcc agggatcggg tcaccgccga taccgacaca ggtcgactga
     6121 ccgaaaccgt aatccgtggt ctgtttaacc gcttcatagg tcagtgtacc ggaacgggaa
     6181 acgataccca ctttacccgg tttgtgaatg tgacccggct ggataccaat tttgcattca
     6241 cccggggtga taacgcctgg gcagttcggg ccgatcatac gaacgcctgc ttcgtccagt
     6301 ttcactttca cggtcagcat atccagcgtc gggatgcctt cagtgatggt gataatcagt
     6361 ttgatgcctg catcgatagc ttccagaatg gagtctttgc agaacggtgc tggtacgtaa
     6421 ataacagacg cggaagcgcc agtagcagca acagcttcac gcacggtgtt gaacaccggc
     6481 aggccgaggt gggtagtgcc gcctttacct ggagttacgc caccaaccat tttagtgccg
     6541 tatgcaatgg cctgttctga gtggaaagtc ccctggctac cggtaaagcc ctggcagata
     6601 accttggtgt ttttatcaat taaaatggac attatttccc ctccactgcg gcaacaacct
     6661 gctgagctgc atccgtcaga ccttttgctg caataatatt caggccgctg tcagccagtt
     6721 tcttcgcgcc gagttcggcg ttgttacctt ccagacgtac cacgaccggt acgttaacac
     6781 ccacttccgc taccgcgccg atgataccgt cagcgatcag gtcgcaacga acgataccgc
     6841 cgaagatgtt aaccagaacg gctttcactt tgtcgtcaga gaggatgatt ttgaacgctt
     6901 cggttacacg ttctttggtt gcgccgccgc caacgtccag gaagttagcc ggttcgccgc
     6961 cgtgcagttt aactatgtcc atcgtaccca tcgccagacc tgcgccgtta accatacaac
     7021 cgatgttacc gtccagcgca acgtagttca gttcccactg tgcagcctgt gcttcacgcg
     7081 gatcttcctg cgactggtca cgcatttcgc gcagatcagg ctggcggaac agggcgttgc
     7141 catcagcgcc cagtttgccg tcgaggcaaa tcagatcgcc ctgtttggtg atgaccagcg
     7201 ggttgatttc gatcagcgcc aggtcgcgct ccaggaaaat ggtcgccagg cccatgaaga
     7261 ttttggtgaa ctgctgaacc agtttacctt ccagacccag tttgaacgcc agctcgcgtc
     7321 cctgatacgg catcgggcca gtcagcggat caagcgcaac tttatggatc aggtgcggag
     7381 tttcttccgc cactttttcg atttccacgc cgccttcggt ggaggccata aagaccacgc
     7441 gacgggaact acggtctaca acggcaccga gatacagctc tttagcgata tcggtcgccg
     7501 cttcaaccag aatctggtta accggttggc cattggcatc tgtttgatac gttaccagac
     7561 gcttgcccag ccagttttct gcaaaagcac ggatgtcttc tttgctgttt acaactttca
     7621 caccgcccgc tttaccgcgg ccaccagcgt gaacctgaca tttcactacc cacggaccgg
     7681 caccgatttt tgaagcggct tcttctgctt cgcgcggagt agtacaggca taacccaccg
     7741 gtgctggtaa gccatagcgg gcaaacagtt gttttgcctg atattcatgt aagttcatgt
     7801 gttctgtcca tccttcagta atcgttatct tttaaaccgt agaccgacac cctatccacg
     7861 tcggtctaca gtgcaggtga agcttaaact actacacgtc cagcagcaga cgcgtcggct
     7921 cttccagtaa ctctttgatc gttaccagga agcccacgga ttcgcgacca tcgatcagac
     7981 ggtgatcgta ggacagcgcc aggtacatca tcggcaggat ctcaacctga ccattcaccg
     8041 ccatcggacg atctttgata gcgtgcatac ccagaattgc gctctgcggc gggttgatga
     8101 tcggcgtaga catcagggaa ccgaacacac caccgttggt gatggtgaag ttaccaccgg
     8161 tcagatcttc aaccgtcagc ttgccatcac ggcctttgac tgccagctct ttgattttct
     8221 tctcgatgtc tgccatgccg agggtatcga catcacgcag aaccggcgtc accaggccgc
     8281 gcggcgtaga aaccgccatg ctgacgtcga aatagttgtg gtaaaccacg tcatcgccgt
     8341 cgatagacgc gttcacttcc gggtaacgtt tcagggcttc aaccaccgct ttcacgtaga
     8401 aggacataaa gcccagacgg atgccgtggc gtttttcaaa cgcttcaccg tactgcttac
     8461 gcagatccat aatcggcttc atgttgactt cgttgaacgt ggtcagcatg gcggtggagt
     8521 ttttcgcttc cagcagacgc tctgccacac gcttacgcag gcgagtcatc gggacacgtt
     8581 tttcactacg tgcagccaga gccggttgcg ccgccggagc agccgctgcc ggagcagact
     8641 ctttcgccgg ggctttcgcc agatgttttt ccacatcttc acgagtcaga cgaccaccca
     8701 caccggtgcc tttaatggcg ctggcgtcga gattgtgttc agccagcagg cgacggatcg
     8761 ccgggcttaa cgcatcgttg ttttgctctt ccagagacgc ctgctggcgt tgcgccggag
     8821 tggacgcttt ctcttcagat ttggcgctgg tttctttacc ggcgctgttg ccttcacgca
     8881 ggcgaccaag gatctgacga gacgttaccg ttgtaccttc atcttccaga accgcatcca
     8941 gaatgccgtc tgctgatgcc ggtacttcca gtaccacttt gtcagtttcg atttctacca
     9001 gcacttcatc acgtacgact gcgtcgccgg gttttttatg ccaggttgcg acggtggcat
     9061 cggctacgga ttcaggcagg tcagggacca gaatatctac gctactcatt gtgtatcctt
     9121 tatttattcg acgttcagcg cgtcattaac cagatcttgt tgctgtttct ggtgaacgga
     9181 catataccct accgccggag aggcggaggc cgggcggcct gcataacgca gagaagcccc
     9241 aaacggaatc acttcacgga aatgatgctg gctgcagtac catgcgccct ggttgagcgg
     9301 ctcttcctgg caccagacaa aatccttgac gtgagcaaac tgctgcaaca cttcctgcat
     9361 cgctttatgc gggaacgggt agagttgctc gatacgcaca atggcgacat cgtgttgatt
     9421 gttcttacga cgctgttcca gcaggtcgta ataaacctta ccagaacaca tcactacgcg
     9481 cttcacgccc ttcggatcaa gctcgtcgat ttcaccgatg gctggcagga aggtgccgtt
     9541 cgccagttct tcgaggctgg aaaccgccag cggatgacgc agcagggatt tcggcgacat
     9601 cacgaccagc ggacgacgca tcccgcgcag cgcctgacga cgcagcatgt ggtaaacctg
     9661 tgccggggta gacggtacgc aaacctgcat gttttgctca gcacaaagtt gcagataacg
     9721 ttccagacgc gcggaggagt gctccggccc ctgcccttcg taaccgtgcg gcagcaacat
     9781 caccagacca cacatccggc cccatttctg ctcgccagag gagatgaact ggtcgataac
     9841 cacctgcgca ccgttggcga agtcaccgaa ctgcgcttcc cagatggtca gggtgcgtgg
     9901 ttctgcggtg gcataaccat attcaaacgc cagcacggct tcttctgaca gtacggagtc
     9961 ccagacacgg aacgcgccct gaccgttatg gatgtgttgc agcggcgtgt aagtggaacc
    10021 gttagactgg ttgtggatca ccgcgtggcg gtggaagaag gtaccgcgac cggagtcttc
    10081 acccgacagg cgaaccggaa tgccttcatc aaccagcgtg gcgtaagcga ggttttccgc
    10141 accgccccag tcgaacagtt tctcaccggc tgccatcgcc tggcgatcgc cataaatctt
    10201 ggcaacgcga gactgcattt caactgcttc tggtaccgta ctgatacgtt tagccagttc
    10261 ctgcaggcgc ttcatctcaa ctttgttcgg gtactcttcg tcccattcgt ggttgaggta
    10321 cggcgaccag gtgaaagagt gcatgttcat cggacgccac tctgccacta cgcaatcgcc
    10381 agcatccagc gcatcgcggt acaggttaac catctcggtg gcatcttcca gcgtcgccac
    10441 tttttcctgc tccagcttgt cagcgtagat tttgcgcggt gtcggatgtt ttttgatttt
    10501 ctgatacatc agtggctggg ttgcgctcgg ctcgtcggct tcgttgtggc cgtgacggcg
    10561 gtagcatacc aggtcgatga agacatcacg tttaaaggtg ttacggaaat cgagcgccag
    10621 acgggtcaca aaggcaacgg cttccggatc atccgcgtta acgtggaaaa tcggtgcctg
    10681 aaccatctta ccgatatcag tacagtacgg tgtagaacgg gcatccagcg ggttagaggt
    10741 ggtgaaacca acctggttgt tgataacgat acgtaccgta ccgccaactt cataaccacg
    10801 cgctttcgac atgttcagag tttcctgaac cacgccctgc ccggtcactg cggcgtcacc
    10861 gtggatggtg attggcagca ctttgttgct gttcggctca tcaagtctgt ccagacgggc
    10921 acgaacagag ccgataacga ccgggcttac aatctcaagg tgagacgggt taaacgccag
    10981 cgccaggtgc accaggccgc catcggtctg gaagtcagac gagaagccca tgtggtattt
    11041 cacgtcgcct gtgccgaggt gttctttatg tttaccggcg aactcgtcga acaagtcttg
    11101 cggtttttta cccagcacgt tcaccagcac gttcagacga ccacggtgcg ccatcccgag
    11161 aaccacttcg cgggtgccgc tgttgccagc gtggcggatc atctctttaa gcatcgggat
    11221 taacgcgtca ccgccttcca gcgagaagcg ttttgcgcca gggaattttg cgccgaggta
    11281 acgctcaagg ccttcagcgg cggtcagttc gcttaagaag cgttttttct cttcgctatt
    11341 gaaagtcgcg cgaccagact cgatacgctg ttggatccag cgtttttctt cggtgctggt
    11401 gatgtgcata tactcggcac caatcgggcc gcagtaggtt tgcttgaggg cggaaataag
    11461 ctcgccgagt ttcatggttt ctttgccgct ggcaaatgaa ccgacgttga aggtttcctg
    11521 gaagtctgct tcggtcagat cgtggaaaga cggatccaga tcggccactt tatcttgctg
    11581 ccacagtccc agcggatcta gattcgcatg ctggtgacca cggaagcggt atgcgttaat
    11641 gagctgcagg actttaacct gcttcacatt ggtgtcaggg tcggagatcg ttgaagagta
    11701 acgtgaagcg tctttcgcca ggcggcggaa atattcacgc gtttgagagt ggaattgatc
    11761 cggtttgact cccgtaccag gtaactgctg gaacgtcgaa cgccagttag cgtcaaccga
    11821 gtcaggatcg gttaagaagt cttcatagag ctgttctatc cagctctggt ttgcgccaga
    11881 gaggtaagaa gagtccaacc aggctttcaa agcgctgttc tgcatcgtga tcccttaagc
    11941 atctttttta tgcttacttc gccgtggata ctaccacgca cagtgatgtg cgtgccgggg
    12001 ttcacttgcg agctcttgtc tacgtagtgg ctcgcgaagg aacctttaaa aactgtctta
    12061 gtgtcagggg cagtttttaa agatttcctg catttctttg ttacgcctga tgcgctgcgc
    12121 ttatcaggcc tgcgggtaat gacttgtagg ccggataagg cgttcacgcc gcatccggca
    12181 ctggttgcct gatgcaacgc ttgcgcgtct tatcaggcct acagtttacg cattacgttg
    12241 caacaacatc gacttgatat ggccgatggc gcgcgtcggg ttcagcccct tcggacatac
    12301 actgacgcag ttcatgatgc tgtgacagcg gaatacgctg aatgcatcgc tcaaaccgtc
    12361 gaggcggctg tcagtctcgg tatcacggct atcgatcagg aaacgatatg ccgctaacaa
    12421 gcctgccggg ccgataaact tatcgggatt ccaccagaaa gacggacaag aggttgaaca
    12481 acatgcgcag agaatacatt catacagccc gtcgagtttt tcgcgctgct ctggcatctg
    12541 taaatgctcg cgagctggcg gattttgtcc attattcaac aggtaaggct taattttctc
    12601 atattgcgca tagaattgtc ccatgtctac caccaaatcg cggatcaccg gtaaacctgg
    12661 cagcgggcga atcacaatct tcttgcccgg ctggttgagt gccgaaatcg gggtaataca
    12721 ggccagacca ttcttgccgt tcatgttcag accgtcggaa ccgcacacac cttcacggca
    12781 ggagcggcgg aacgacaggc tgggatcttt ctctttcagc tggataagcg catccagcag
    12841 catcatgtcg cgaccttctt ccgcttccag ggtgtaatcc tgcatacgcg gagcatcatc
    12901 aacatccggg ttatagcgat aaattgaaaa ctcgagtctc attttcctgt ctccgcatta
    12961 gtaagtacga atcttcggcg ggaatgccgg gcgcagtttc ggttccatgt tgacgcttcg
    13021 gcgcgtcatg gattccgact caggcagata cagggagtgg cacagccagt tttcatcatc
    13081 acgatccggg aagtcgaagc ggctatgtgc gccacggctt tcggtacgga agttggcaga
    13141 aacagccgtt gcatacgccg tttccatcag gttatccagt tccaggcact caacgcgctg
    13201 ggtgttgaac tcgctggaag tgtcatccag acgggcattt ttcagacgct cgcggatcac
    13261 tttcaactgc tcaagccctt tcgccatcgc atcaccttca cggaagaccg agaagttatg
    13321 ctgcatacat tcttgcagcg ctttacggat cgccaccgga tcttcaccgt tacgattatt
    13381 gttccaccgg ttcaggcgat ccagagacgc ttcaacatca gactcgctgg catcgtgcag
    13441 tgcgccctgc tcggcgatag actcttgcag atgcagacct gccgcgcgac caaagaccac
    13501 caggtccagc agcgagttgc cgcccagacg gttagcgccg tgtaccgata cacaagcgat
    13561 ttcaccaacg gcaaacagtc ccggaacaac cacatcttcg cctttctcat tcacagtcag
    13621 cgcctgaccg gtaactttgg tcggaatacc gcccatcatg tagtgacagg ttgggataac
    13681 cggaatcggc tctttcaccg gatcgacgtg agcgaaggta cgggaaagct ccaggatacc
    13741 cggcagacgg gattcgagaa cttctttacc caggtgatcg agtttcagtt tcgcgtgtgg
    13801 cccccacgga ccatcacagc cgcgaccttc acggatttca atcatgatgg aacgcgccac
    13861 cacatcacgg cccgccaggt ctttggcgtt cggcgcataa cgctccataa agcgttcgcc
    13921 atgtttgttc agcagataac cgccttcacc acggcaacct tcggtgacca gtacgcccgc
    13981 accggcaatg ccggtcgggt ggaattgcca catttccata tcctgcaccg gtacgcctgc
    14041 gcggatagcc atgccgacac cgtcgccagt gttaatgtgg gcgttagtgg tggactgata
    14101 aatacgccct gctccgccag tcgccagcac ggtagcgcgg gctttgaaat aaaccacttc
    14161 accggtttcg atgcacagtg cggtacaacc caccaccgcg ccatcctggt ttttcaccag
    14221 atccagcgca taccactcgg agaaaatggt ggtgtggttt ttcaggttct gctgataaag
    14281 cgtgtgcaac agtgcgtgac cggtacggtc agcagccgcc gcagtgcgtg ccgcctgctc
    14341 gccgccgaag tttttcgact gaccgccaaa cggacgttga tagatacgac catcatcgag
    14401 acgcgagaac ggcaggccca tgtgttcgag ttccagaatc gcttccggcc cggttttaca
    14461 catatattca atcgcgtcct ggtcaccgat atagtccgac ccttttacgg tgtcgtacat
    14521 atgccattcc cagttatctt catgggtatt acccagcgca acggtaatgc cgccttgcgc
    14581 agaaacggta tgggaacggg tcgggaagac tttagagagc agggcacagg tctggccgct
    14641 ctgggaaatt tgcagcgccg cgcgcatacc tgcgccaccg gcaccaatca caactgcatc
    14701 aaattctctg actggcaatt tcatcacaca ccccacacca caacgaatcc ataaatcacg
    14761 taaaccacca gtgcaacgac aatcaccagt tgcagcatca ggcgcaaggc cagcggttta
    14821 acgtagtcgg tcaacacctg ccacatgccg atccaggcat gaatcaagat agaaaacagc
    14881 gccagcaggg tgaacacttt ggtgaacgca gaggcgaaga aaccgatcca gacttcatat
    14941 gtcagttcgc cactggtggc gaaaaaaccg accatataaa tgatgtagag cgtcaggacg
    15001 atagcggtag cgcgaacgag gatgaaatca tgtacgccat tgcgtcctaa tgcggaggcg
    15061 ttgcttacca tacgaggact cctgcgagaa gtgaaagcac gacagtaata acaaaggaga
    15121 ttttggcgga gcgtttaccc gcttcgaatg tttcttccag atagccaaaa tccatcatca
    15181 tgtggcgaat acctacgacg acgtgatacg ccagagcggt aaggatgccc cacatgataa
    15241 atttgacgaa gaagctgccc ataatcgcgg aagcttgctc gaaaccttca ggggaagaga
    15301 ggctggtacc cagaagccac tgcaggatgc ccactgcaac aaaggtgatc acaccggaaa
    15361 cgcgatggag aatggacgct atcgccgtga tggggaaccg gatggtctgt aggtccagat
    15421 taacaggtct ttgttttttc acatttctta tcatgaataa cgcccacatg ctgttcttat
    15481 tattccctgg ggactacggg cacagaggtt aactttctgt tacctggaga cgtcgggatt
    15541 tccttcctcc ggtctgcttg cgggtcagac agcgtccttt ccataactgc gcgtcatgca
    15601 aaacactgct tccagatgcg aaaacgacac gttacaacgc tgggtggctc gggattgcag
    15661 ggtgttccgg agacctggcg gcagtatagg ctgttcacaa aatcattaca attaacctac
    15721 atatagtttg tcgggtttta tcctgaacag tgatccaggt cacgataaca acatttattt
    15781 aatttttaat catctaattt gacaatcatt caacaaagtt gttacaaaca ttaccaggaa
    15841 aagcatataa tgcgtaaaag ttatgaagtc ggtatttcac ctaagattaa cttatgtaac
    15901 agtgtggaag tattgaccaa ttcattcggg acagttatta gtggtagaca agtttaataa
    15961 ttcggattgc taagtacttg attcgccatt tattcgtcat caatggattc tttacctgca
    16021 agcgcccaga gctctgtacc caggttttcc cctctttcac agagcggcga gccaaataac
    16081 aaacgggtaa agccaggttg atgtgcgaag gcaaatttaa gttccggcag tcttacgcaa
    16141 taaggcgcta aggagacctt aaatggctga tacaaaagca aaactcaacc tcaacgggga
    16201 cacagctgtt gaactggatg tgctgaaagg cacgctgggt caagatgtta ttgatatccg
    16261 tactctcggt tcaaaaggtg tgttcacctt tgacccaggc ttcacttcaa ccgcatcctg
    16321 cgaatctaaa attactttta ttgatggtga tgaaggtatt ttgctgcacc gcggtttccc
    16381 gatcgatcag ctggcgaccg attctaacta cctagaagtt tgttacatcc tgctgaatgg
    16441 tgaaaaaccg actcaggaac agtatgacga atttaaaact acggtgaccc gtcataccat
    16501 gatccacgag cagattaccc gtctgttcca cgctttccgt cgcgactcac atccaatggc
    16561 agtcatgtgt ggtattaccg gcgcgctggc ggcgttctat cacgactcgc tggatgttaa
    16621 caatcctcgt catcgtgaaa ttgccgcgtt ccgcctgctg tcgaaaatgc cgaccatggc
    16681 cgcgatgtgt tacaagtatt ccattggtca gccatttgtt tatccgcgca acgatctctc
    16741 ctatgccggt aacttcctga atatgatgtt ctccacgccg tgcgaaccgt atgaagttaa
    16801 tccgattctg gaacgtgcta tggaccgtat tctgatcctg cacgctgacc atgaacagaa
    16861 cgcctctacc tccaccgtgc gtaccgctgg ctcttcgggg gcgaacccgt ttgcctgtat
    16921 cgcagcaggt attgcttcac tgtggggacc tgcgcacggt ggtgctaacg aagcggcgct
    16981 gaaaatgctg gaagaaatta gctccgttaa acacattccg gaatttgttc gtcgtgcgaa
    17041 agataaaaat gattctttcc gcctgatggg cttcggtcac cgcgtgtaca aaaattacga
    17101 cccgcgcgcc accgtaatgc gtgaaacctg ccatgaagtt ctgaaagagc tgggcaccaa
    17161 agatgacctg ctggaagtgg ctatggagct ggaaaacatc gcgctgaacg acccgtactt
    17221 tatcgagaag aaactgtacc cgaacgtcga tttctactct ggtatcatcc tgaaagcgat
    17281 gggtattccg tcttccatgt tcaccgtcat tttcgcaatg gcacgtaccg ttggctggat
    17341 cgcccactgg agcgaaatgc acagttacgg tatgaagatt gcccgtccgc gtcagctgta
    17401 tacaggatat gaaaaacgcg actttaaaag cgatatcaag cgttaatggt tgattgctaa
    17461 gttgtaaata ttttaacccg ccgttcaaat ggcgggttaa tttttatatg cctaaacaca
    17521 aaaaattgta aaaataaaat ccactatcgg acattcatat attctaaaaa aaaacatcgg
    17581 tataaattat aaacaaatag atgattttta atcatctatc ttcctctcag ttacatgaat
    17641 aaaccaccaa atatag
//
LOCUS       DAZPCS010000075        17643 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZPCS010000075 DAZPCS010000000
VERSION     DAZPCS010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17643
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.075"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(1..671)
                     /gene="chbR"
                     /locus_tag="ACHUQ7_002392"
                     /pseudo
     CDS             complement(1..671)
                     /gene="chbR"
                     /locus_tag="ACHUQ7_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
     gene            complement(679..1029)
                     /gene="chbA"
                     /locus_tag="ACHUQ7_002393"
     CDS             complement(679..1029)
                     /gene="chbA"
                     /locus_tag="ACHUQ7_002393"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFR1135768.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(1088..2436)
                     /gene="chbC"
                     /locus_tag="ACHUQ7_002394"
                     /pseudo
     CDS             complement(1088..2436)
                     /gene="chbC"
                     /locus_tag="ACHUQ7_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
     gene            complement(2521..2841)
                     /gene="chbB"
                     /locus_tag="ACHUQ7_002395"
     CDS             complement(2521..2841)
                     /gene="chbB"
                     /locus_tag="ACHUQ7_002395"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFR1135769.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(3140..3478)
                     /gene="osmE"
                     /locus_tag="ACHUQ7_002396"
     CDS             complement(3140..3478)
                     /gene="osmE"
                     /locus_tag="ACHUQ7_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFR1135770.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            3680..4507
                     /gene="nadE"
                     /locus_tag="ACHUQ7_002397"
     CDS             3680..4507
                     /gene="nadE"
                     /locus_tag="ACHUQ7_002397"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFR1135771.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETRNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            4737..5624
                     /gene="cho"
                     /locus_tag="ACHUQ7_002398"
     CDS             4737..5624
                     /gene="cho"
                     /locus_tag="ACHUQ7_002398"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFR1135772.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(5584..6159)
                     /gene="ves"
                     /locus_tag="ACHUQ7_002399"
     CDS             complement(5584..6159)
                     /gene="ves"
                     /locus_tag="ACHUQ7_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFR1135773.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGVCWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(6362..6847)
                     /gene="spy"
                     /locus_tag="ACHUQ7_002400"
     CDS             complement(6362..6847)
                     /gene="spy"
                     /locus_tag="ACHUQ7_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFR1135774.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(7177..8145)
                     /gene="astE"
                     /locus_tag="ACHUQ7_002401"
     CDS             complement(7177..8145)
                     /gene="astE"
                     /locus_tag="ACHUQ7_002401"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFR1135775.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(8149..9470)
                     /gene="astB"
                     /locus_tag="ACHUQ7_002402"
                     /pseudo
     CDS             complement(8149..9470)
                     /gene="astB"
                     /locus_tag="ACHUQ7_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
     gene            complement(9467..10945)
                     /gene="astD"
                     /locus_tag="ACHUQ7_002403"
     CDS             complement(9467..10945)
                     /gene="astD"
                     /locus_tag="ACHUQ7_002403"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HFR1135776.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLASLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVGGVPDEEVFGPFLRVWRYDTFDEA
                     IRMANNTRFGLFCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(10942..11976)
                     /gene="astA"
                     /locus_tag="ACHUQ7_002404"
     CDS             complement(10942..11976)
                     /gene="astA"
                     /locus_tag="ACHUQ7_002404"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFR1135777.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(11973..13193)
                     /gene="astC"
                     /locus_tag="ACHUQ7_002405"
                     /pseudo
     CDS             complement(11973..13193)
                     /gene="astC"
                     /locus_tag="ACHUQ7_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
     gene            13639..14445
                     /gene="xthA"
                     /locus_tag="ACHUQ7_002406"
     CDS             13639..14445
                     /gene="xthA"
                     /locus_tag="ACHUQ7_002406"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFR1135778.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            14612..15322
                     /locus_tag="ACHUQ7_002407"
     CDS             14612..15322
                     /locus_tag="ACHUQ7_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFR1135779.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFINAPLFLLPGSILVIAGGIVFGPLLGTQLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGFTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILIQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            15327..16004
                     /gene="ydjY"
                     /locus_tag="ACHUQ7_002408"
     CDS             15327..16004
                     /gene="ydjY"
                     /locus_tag="ACHUQ7_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFR1135780.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYAPPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKHGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            16019..16726
                     /locus_tag="ACHUQ7_002409"
     CDS             16019..16726
                     /locus_tag="ACHUQ7_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFR1135781.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTELFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            16726..17274
                     /locus_tag="ACHUQ7_002410"
     CDS             16726..17274
                     /locus_tag="ACHUQ7_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFR1135782.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            17284..17610
                     /locus_tag="ACHUQ7_002411"
                     /pseudo
     CDS             17284..17610
                     /locus_tag="ACHUQ7_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         4365 a         4685 c         4308 g         4285 t
ORIGIN      
        1 tcattaataa tctgcattgg tgttttgcca taatatcgtt gagtcgctcg cgtcaaatat
       61 tcctgtgatt tggctgacaa cgtcaccata ttctccagcg ccgattcact aaactgctct
      121 ttatcatgca tcttttctac cgtacttttc agccactacg gtacatcatc aatcacctgt
      181 tcttcgcggt aatgacgtaa acggttaatg acataaaaag taaccatctc aacaaactct
      241 tccagccctg tttcgcggaa atttaatgaa gaaatcactg tttccacata ggtgagaaaa
      301 gcgttattgg tccggtatac ctgcgaagca acaaagcaat aaggcaacaa cggcaggtaa
      361 tgctgctcaa aaaagcgttt actgatccca acgttcaata tgcgcgtggc accaaactca
      421 taaaaacttt gatggtgcga acctaacgga ataaaaacaa aatcgccccg ttccagtaac
      481 acgcgcttac cgttaatctc ctggaaataa cgcccggtta ataccagagt aaattcataa
      541 tagtcgtgct gatgcagtcc gctgatactc tcagttttgt tatagataaa cacatggaag
      601 tttttgccat taaacaactg ctgttctcgg gcagtggcaa tttccggcgc gttaatcact
      661 ggctgcatca tcgactcctt atgccttcag tttttcatga agctcaatta attcagtaat
      721 cagttcacgc gcaagcatgg acgtcattaa atgatcctga gcgtggacca gcaccagact
      781 cactttcatc ttaccttcgc ccgcatcgcc ttcaatcagt ttcgtctgta ccagatgcgc
      841 ttcattcaat gccatccgtg actgatccat catggctttc gctgcggcaa aatcgccctg
      901 cttcgcctgt ttcaatgcgg catacgccag gctacgcgct tgtccagagt tgatgataag
      961 ccccatcacc acttcttcca gctcttcagc ttctgtttgc gtatcaggaa tattatcgag
     1021 atccatcata catcgttcct ctctcttctt accggcaaga ttacccgtac cggcatcgat
     1081 taaaatttca ggggttagcg atatcttctt cgctctcttc tttatcaatc gcattttgtg
     1141 ctttgttagc caccacaaca aagggcagat aaattaacgt tgcgatgcca aggttgaaga
     1201 gtgcgaccag caatgcggcg acgctaccgt tggtgttaaa gaaggctccc agaccggttg
     1261 gcatggtcca cggtgcaata ttggtcaccg gaggaatgat gcccatgtag tacgctgcga
     1321 gggtgattgc cgccagaatc ggttgtacca gtacaaacgg gataaacatc accgggttca
     1381 tgataattgg cagaccaaac agaatcggtt cgttaatctg gaagatgccg gacggcagcg
     1441 ccagttttgc cacctgacga taatcagcac ggcgagaggc gataaagata gccaggatca
     1501 ggcctaaagt cgcaccactg cccccaagga aaataaagga gtccagcatc ggcttggccc
     1561 agatatggaa ggtcttaccg gctgccagcg ccgcttcgac ggaaccatat tgctgatagg
     1621 tcgcgatatt ttccagcgcc cacggcgtca taatgccgtt gtccagtgcg gtcagcgcca
     1681 gcgcgccatg aataccgaag aaccagagca gtggaacaaa gatcacatag gcccagccca
     1741 ccacgctacc caacgatgcc agtggggttg agatggtatc cataatgatc tgatggaagt
     1801 tggtgcccca ggtattcaac gcccaggcaa taatccccat cacggaaaga ataataaagc
     1861 cgggaattaa tgccgagaaa gaacgcgata ctgaagcagg tacgctgtcg ggcagtttaa
     1921 tgacccaatt gcggcggaca ataaaggtaa acatttctgc caccaccagg ccaataataa
     1981 tcccggagat gatattcgcc ccacctaacc agtttgcgcc aaccgcatag gcctcaccga
     2041 cactatatgg ggtgacggtc ataaatgctg caacggataa caacccagcc gccagcgcat
     2101 cgactttacg ctcttctgcc agcgccatgc caataaagaa cggtgccatt aaagacatta
     2161 ttcctaatgt tccgttatat acgttgccgc caataccttt cagaccatta agtgtttcaa
     2221 tggttgaggc gtcgaggcga atacctaagg aataaaaaac gacccctccc caaagcttag
     2281 aaaaacgttg ttaattaata caaacatggc ccccgcaagg gttaacggca ttaagcgaat
     2341 aaagccattt ttgattgcat taacgtgtgg ctgctttcct attttaactg caaaagggag
     2401 gagtaccttt tcaagcgatg caataacatt actcatagaa aaataccctt aaaaaccgca
     2461 atttaaatat tacggtattg atttatgaaa taactctttg acgggaaaat ttaaaataaa
     2521 ttaatttgct gcggcttttt taatcgctgc aaccgcagcc ttaagcacgc ctaaaccatc
     2581 gactttgcca taaagcagcg agtcaattac ttcaaccggt ttgttgggta acaaacgctg
     2641 gatttcgggc aacatataag caatctgcgg ccctaataac acgacatcgg cattctgacc
     2701 tttttcacca gccagtgttt ccggaaatgc ttcaataatg accggaactt catatttttc
     2761 tgcctgtgcg cgcatttttg ataccagtaa agagatagac atgcccgcag aacaaaacag
     2821 ataaatgtgt ttcttttcca taaaactgcc ctcatcgacg attatctgtc agccagacac
     2881 tccgcaagcc ttgacctgct tccatgctct gggtaacttg cgaaaccaac atgacttttt
     2941 ttgtggctga aaggagtata cggtatagac ctgagcaata gtatttcttt gaccttataa
     3001 attgtttctc cttgacctgt ggttatgacc ctcttcacat ttcgggcaaa taattcgcga
     3061 agataattaa gaactcaggc caaaaaaaac cggcgcaatg gccggtttcc gttgttactc
     3121 aagctttcag acgaattgat tacttcgcag cctgtggatc agtgtcgtat tcagcacagg
     3181 tctgataacc ggagttgatg acatgtccgg tatcatctaa cgcgacaaag taggtttctg
     3241 ctttaccatc acgttgaccc aggatgtagg tctggcaagt accgcgagca tggatcatgc
     3301 tcacttcaga cgaaggttta cccgcaattt gtgcaacctg cgcccggctc atgccttttt
     3361 tgacgtcttt caccacaggc tgtacaaact ggtctttggt acgatcataa gctgtacaac
     3421 ccgccagcat ggttaatacc gccgctgcac tcagaattcc tgccatattc ttgttcatat
     3481 tccgtcctct tgtttatcag cgtgttagat aagcctggaa tacattgggc gctttttcaa
     3541 gcccgtgaac gaaacggctc cgctttcaga ggattcctgt atgacgtttt aaccaccatt
     3601 cagcccgctg tcgcttgtcg tttcagtagc aacgggttag ctttaaggaa gttttgtctt
     3661 ttctgtctgg aggggttcaa tgacattgca acaacaaata ataaaggcgc tgggcgcaaa
     3721 accgcagatt aatgctgaag aggaaattcg tcgtagtgtc gattttctga aaagctacct
     3781 gcaaacttat ccgttcatta aatcactggt gctcgggatc agcggcggtc aggactccac
     3841 gcttgccgga aagctgtgcc agatggcgat taatgagctg cgccaggaaa ctcgcaacga
     3901 atcactgcaa tttattgccg tacgcctgcc ctatggtgtt caggctgacg aacaagattg
     3961 ccaggatgcc attgccttta ttcaaccgga tcgcgtatta accgttaata tcaagggcgc
     4021 ggtattggct agcgagcagg cattgcggga agcaggcatt gaactgagcg attttgtccg
     4081 tggcaatgaa aaagcgcgtg agcggatgaa agcacaatat agcattgcgg gtatgaccag
     4141 cggtgtcgtg gtgggcaccg atcatgcagc agaagccatt accggattct tcactaaata
     4201 tggtgacggc ggtacggata ttaatccgct gtatcgtctc aacaaacgcc agggtaaaca
     4261 gttactgacg gcattaggtt gcccggaaca cctttataag aaagccccaa ctgccgatct
     4321 tgaagatgat cgcccttctc tgccagatga agtggcactc ggcgtgacct atgacaatat
     4381 cgacgactat ctggaaggga aaaacctacc tgaacaggtc gccagaacaa tagagaactg
     4441 gtatctgaaa accgaacata aacgccgtcc gccaattaac gttttcgatg atttctggaa
     4501 aaagtaataa tttgcaccta ttggattgtg cgaaaaagag taatttgttc acgccggatg
     4561 cggcgtgaac gccttattct acctacaaaa ctatgcaaat tcaatacatt gcaggagtcg
     4621 aataggcctg acaggcgtag cacgtcagac ggtgtaacct ttgtcatcaa cccgcctctt
     4681 ttttaatcgc ctcccgcctg ttacactgga tagataacca gcattcggag tcaacagtgg
     4741 tacggcgttt aacttctccg cggctcgaat ttgaagctgc ggcaatttat gaatatcccg
     4801 aacatttaca ttcattcctt aatgacttac ccacccgacc aggggtgtat ctgtttcatg
     4861 gcgaaagtga caccatgccg ctctatatcg gcaaaagcgt taatatccgc agtcgggtgc
     4921 tttctcattt acgcaccccg gatgaagccg ccatgctacg gcaatcccgg cgaattagct
     4981 ggatctgcac cgcaggcgaa atcggcgctc tgctccttga agcgcgatta atcaaagaac
     5041 aacagccgct gtttaataaa cggttgcgcc gcaatcgcca gctctgtgcc ctgcaattaa
     5101 atgaaaagcg cgtcgatgtg gtgtatgcca aagaggtgga tttttcacga gcccccaacc
     5161 tgtttggcct gtttgccaat aggcgcgcag ctttgcaagc attgcagagc atcgctgatg
     5221 aacaaaaact ttgttatggc ctgttgggac tggaaccgtt aagtcgcggt cgtgcatgtt
     5281 ttcgttcagc gctaaaacgt tgcgccggag catgctgcgg taaagagagc catgaggaac
     5341 atgcgctacg cttgcgccag tctctggagc gtttgcgagt ggtgtgttgg ccttggcaag
     5401 gtgcggtggc actgaaagaa cagcacccgg aaatgactca atatcatatt attcaaaact
     5461 ggctgtggct gggggcggtt aattcgctgg aagaagcgac aacgttaatt cggacacccg
     5521 ccgggtttga tcacgacggt tataaaattc tttgtaagcc gctgctttcc ggtaactatg
     5581 aaattactga acttgatccg gcgaatgacc agcgagccag ttgatttcac tgaacaacag
     5641 tttaccttgc ggttgtaaaa gccgcagggt gtgcctgcca tcgaaccagc acacgccctg
     5701 gtcggtagtg agcagtttgt cgcccagttg ccacgccccg ttaatcacaa acaccacccc
     5761 gccgcgtgag ccaaaggtag taaacgtacg ttcggcaatt ctgactttcg ccttacagac
     5821 atccaggcgc gtcataatat tgaaatccat cgacatctgc cctgccgtca gtttcgcttt
     5881 taccacctgg tccgccgtaa aggcaaaagg ctgtaacggt tttaaggtat ggttaaagcg
     5941 gtctgcgctt tcaaggagca tctcgccgcc ttccagcaac gtcactatcc tttccatgcc
     6001 gggaaacaaa gaaaactccc cattcgccgc gatggatgca atgctggcac gccagtaaaa
     6061 atcacgtttt gccggtggga aagtgcaaat ttcgcgcgtt tcaccggcag cgtttcgcca
     6121 cagattcacc gacattttac gcatatcaaa gtattccata ctcgctcccg gcccgttctt
     6181 ttcattgtta tcgttcaggt aaccgtggat ggttatcggc aactcgggtg atttacgtta
     6241 gtggtgatca ggcttttttt acaactgttt gagaagagaa aagaaaaccg ccgatcctgt
     6301 ccaccgcatt actgcaaggt agtggacaag accggcggtc ttaagttttt tggctgaaag
     6361 attattcagc agttgcaggc attttacctt ttgccgctgg acgttctgtc agacgcttct
     6421 caaaattagc attaaattgc tttttctgtt ccggcgtcag gatgttgtaa attttgttct
     6481 gggtttccat gtgcgccagc atgttagctt tgcgctgttc ttccattttt gcgatctgcg
     6541 cttcagcttt tgctttatcg aaggtatcgc tggcaatgat gtcatgcatt gcgcggcgtt
     6601 cttccagcgg cggacgtttc atctggtcac gctggccttt catgatttcg tggatctgct
     6661 gtttctgcgc gtcggtcagg ttcaggtctt tgaacatcat gtcctgatgc ggaccgaact
     6721 tgcctttgtg gtgcatcatc ggcttcgcgt cagccggtgc tgcggtagtg gtgtctgcgg
     6781 catgggccag gttagccgcg ccaagagcca gggtagaggc aacaaacagt gcagttaatt
     6841 tacgcatatt ctatatcctt cctttcagtt atttattacg gctttcttaa gtagcgtgcc
     6901 gtgttgacga gattaacttt actgacttta gcgtcaatta atcagagcaa cggtaaaaca
     6961 atgaaagtgt aaaaaacact tttgcgccaa ttatggagaa aaaaagaaaa tttgatggag
     7021 agtgatgaga gaatattaca acacgatgat tttgcagaga ttatgaagaa ctataccgga
     7081 tgactggtga taaataaagc aaataaccag gattaatctg tattaattta taagaaagca
     7141 acttaatacc cgcagaatga tttctgcggg taagtattag cttatttttt cgagcattaa
     7201 tcccgcgcgt aatcccaacg ctaccaacgg attagggaat aacacatact ctacatcatg
     7261 ggttacggta aaacgttcct ctccgtcctg cgccagcaat gttcctttct caaacggcat
     7321 aaaattcagc gtgtcacttg ccatatgcat ttcgaaggac ggcgagtggc gagtaatttg
     7381 cgaaaccacc cgataacgga gcggcggtgt tctcacgata ccgacactct caccagatag
     7441 cagcgcagca attgcgctgg cagttactgc aaactggcga agatcgtttt gcccaaaggg
     7501 caacgctttg ccaagttcca acgtacaggc cagcgcgcca aaatgtctga cgctgaaatg
     7561 ggtaaacgta ccaccaggct cctgatggaa caccagcgcc tccagccccg ccgcacccag
     7621 ccacgtcaga aatttctcgt cccagggaat gtcgcgttgc ggtaatacac cgaactgcgg
     7681 atgcaaggag ccacgaattg cggtatgtag atcaaggtgc caacgcacag attctttgcc
     7741 ctggtcataa aaatcttcca ggcactgttc cagttcgcgc gcccgacagg tttctccgct
     7801 ttcagcaaat agctgccaac gaccgccaaa cattcggttc atatcgctat ggcaataacg
     7861 tttcccttgc ttcagcgcag gaggattccc gaggatcacc agcaaccgcc aacgtaacgg
     7921 gatctcgccg tgagatatcg cgccaagcaa cgcgtccagc atttccacag gtgccgtctc
     7981 attaccgtgg atccccgcag aaatcaccag cgcgccttgc ggtggcgtta atggcgtcag
     8041 ttccagcaca ccatcgccca gccagcgcca gcgaacgccg ttgatttctc gctcggtgat
     8101 aaccggtttt ttacccgtta aggtcagagc aagaaaatta tccattgccc ctccctcgcg
     8161 ctggaacgga taaaccgaac cgagattcag taattggctc aatacatcca gtgcttcccg
     8221 cccttcgcgc agcaattgcg ggtcggccag atcggcagca gtaagacgat cgcggtagta
     8281 acgatccacc cagtcattga gcgcattaaa caaggtatcg ttcatcatca ccgccggatt
     8341 caccgcccgg cgttcttctt ctgtcaatac cacccgcaac cgcaggcacg ccgggccgcc
     8401 gccattcgcc atgctttcac gtaagtcaaa gacttttagt tcgctaatcg ggttgtcagc
     8461 ggcaaggagt tcattgagat aaccccatac tccggcgtgt tcccgacact cctgaggcag
     8521 caccaacatc atggaaccat catcgcggct caacagttgg ctgttaaaca gataggtaga
     8581 caccgcatcc gacacggaaa cctgagttgc cggaacttct atcgccataa aaccgttcac
     8641 ccgcgtgcca gtaactgtga ctggcgagcg aacgcctgtt ggtggcaaaa cagcacctgg
     8701 cggttactca cggcaatcac gtcattatga aaaacgctct ggtcgataac gtccgggttt
     8761 tgctgggcga aaatcacctg ttggggattc acctgattaa gccttgccac cgcctcgctg
     8821 gcttcgcgag tctgtcgcgc cggataacgg gaaggccggg tatcattgcc ttcttctcgc
     8881 ccgtagacaa aaagtagcat acccggttca ccgtaatgac cgccgagacg attgtggttt
     8941 gccgccccct catcaccgag caacgctacc tgcggcaacg ccgaatggac gctaaatttc
     9001 tcttcgtcct taaaaatcgc ttttaacagc gattcagtga cgggcgcttc cagcgaacgg
     9061 tgaaatttat tgttcaggtt ggcaacggtg agatgcactt tgccatccag cgtatcggca
     9121 gatggcgcga tcgttgccgc attggctacc cacattggcg aagcggaact gacgctggaa
     9181 agccagtgcg gtgcctggcg tgcaactttt tccagtacct gctcatcgct accactgaat
     9241 cccaactgac gcagcaccgg aataaacgga cgctcgtgcg gcggaatcac ggcctggggg
     9301 aatcccgcat cggcaagggc tttcattttc agtaagccct gttttgccgc cagtcgcggg
     9361 ttagacacct gaaaacggtg acgggtagag gcttcattac caaacgacag gcccgcgtaa
     9421 tgatgcgtca gccctaccag cccgtcgaaa ttaacttccc aggcgttcat cgcaccacct
     9481 catcggaaaa atccagcccg gggttaagcg tggcgggcaa tgttaacgag tccgactcca
     9541 ggctcgccat aggccatgcg cagtaatctg cggcatacca ggcgctgggg cgatggttac
     9601 cggaagcacc aatgccgccg aatggggcgg tactggcagc accagtaagc ggtttgttcc
     9661 agttaacaat ccccgcccgc gcctccagca acagttgatc gaatttttcc cgctcggggg
     9721 aaaccagacc gcaaaagagt ccgaagcgag tgttgttcgc catccgaatc gcctcatcaa
     9781 aagtatcata acgccagacg cgcaaaaacg gcccgaacac ctcttcatcc ggcacaccgc
     9841 caacgcccgt catttcaatg atccctggtg tcagcaacga tgtccctgct tgtaataagc
     9901 gcggcgcaag cagggttcgt ccgcccatcg cttccagttg ctgccaggca gtaaccacct
     9961 gctgtgcggc ctgttcagaa atcagcccgc caataaacgg ctgcggttcg tcatcccagt
    10021 tgcccggcgt taatcgctgg ctgacggcaa ccagactagc aagaaacgca tcgccctgcg
    10081 ctccgctttt gagcaataaa cggcgggcgc aggtgcagcg ttgcccggct gtgacaaacg
    10141 ccgactgaat ggtcagatgg acagccgcgt cgatatccgc cacctcatcg ataattagcg
    10201 gattattacc gcccatctca agggcgagaa ttttctccgg ctgaccggag agctggcgat
    10261 gcagctggta gccggtattg gcgctaccgg taaacagcaa accgtcgaga tcctccagcg
    10321 cactcagcgc ctgacccgtt tcacgcccgc cctgcaccag gttcagcacg cccggcggca
    10381 agccagcctg ctgccataaa cgcattaccg cttcgccact ccacggtgtc agttcgctgg
    10441 gtttaaagat aatggtgtta cctgccagca atgccggaac gatatgtccg ttcggcaaat
    10501 gaccagggaa attatacggc ccaaacaccg ccagcacgcc gtgcgggcga tgtcgcaggc
    10561 tcgccgcacc gtccggcatt tcactacgct gctcgccggt acgaacgtga tacgccttaa
    10621 ttgatatcgc gattttattg atcatcgccg tcacttcggt tgccgcttcc cagcgcggct
    10681 tacccgtttc tctggcaata atcgcggtta attcggcttt attgctttcc agcaatccgg
    10741 caaagcgttc gacaacgacc tgacgttcag ccaatgagag ccgcgcccag cgcggaaacg
    10801 ccgcacgggc tgcccgacaa gcctgttcga cctgagcggc atcggcatca ttgccttgcc
    10861 ataacacctc gcccgatacc ggattacgct tcacacgcga tgcgccctgg cccgttatcc
    10921 agtcaccgtt aatccataaa gtcatgctgt tttctcctct gcgcacaggc gcaccagacg
    10981 aacgcgatcc cctgcgtggc atttgagggc atccagttgt gcggcggtta aaatcaaacg
    11041 ctcggttgcc ggatcggtac gcgccagcac cacgcggaaa tggtgataat tttcattggc
    11101 gaccaggcag gccggaaaat cgccctgcgc aggctgccct tctgccactt ccaccagccg
    11161 acttttacgg atggcgcgca cgcggtcgat gtcacactca agcgttggcc cgccgtcgaa
    11221 gatgtcgata tagttgcggt aacgaaaacc ttctttctcc agcaccgcgc gggcaggcgc
    11281 ggtttgcgga tgtacctgac cgatgacgtc ctgggcttcc tgggataaaa agtgggtata
    11341 gatcggatgt ttcggcatca gttctgcaat aaatgccttt tgcccggtgc cgcagagaaa
    11401 atcggcgcgg ctaaaatcca tcgaaaagaa gcgtttaccg aggctttgcc agaacggtga
    11461 atagccgtgt tcgtcaatca ccccgcgcat ttcagcaacc actttgtcat taaacttgtc
    11521 gcgaaaagcc gccataaaca taaagcgcga tttcgacagc aaatagccgt tgccctcttt
    11581 gcgccagtcc gggtcgagaa acagcgtgca cagctcgctg ctgccggtgt gatcgttact
    11641 aagaaacagc gtcggcaatg cgttatagac attcagctct tttgaggcgt gaaccaacgt
    11701 gccgacgcga tagttgtacc agggatcgtt cagcccaacc gccacctcaa tagcgcaaat
    11761 ccccgccacg gtgcctgtct cgctatcttc cagcacgaac acatagccct gctcgctttt
    11821 gggcagttca ccttgccagg ttttgattgc cctttcgata cgcgccgaaa gcgtggcttc
    11881 attggcggga agcgacgtca ggccgccgcc cgttttgctg gcaagctgca tcagcgccga
    11941 gacatctgag cgctcaacgg gacggatgac catcatgatg aacctcggct aacaaagtgt
    12001 tcgcaagcag ctgcaaagcg atccagtccg gtcgtcacct cttcttcgct gacattgagc
    12061 gcaggcgcaa aacgcaccac gttgccaccc gcaatcagta ccatcacgcc tgctttcgcc
    12121 gcttccagag agatctgttt cgcttgcccg gcgtaatcgg cattcagtac acagccaatc
    12181 aacaaaccta agccgcgaac ttcactgaac aaaccatagc ggtgattaat agtattaaga
    12241 cgctcaacaa accagtcgtg acgctgttta acgccattaa gcatctctgg tgtgttgatg
    12301 agctccagca ctttgcctgc taccgccgag gccagcggat taccgccata ggtggtgcca
    12361 tgagtgccaa cggtcatcac gcgggcgcac tcttcggttg ccaacaacgc accgaccggg
    12421 aaaccgccgc ccagcgcttt ggcggtggtt aacagatcgg gcgtcacgcc gtagtgcata
    12481 taggcataca gctcaccagt gcgcccgacg ccggtttgta cctcatcaaa aatcagcaag
    12541 gcattgtggc ggtcacacaa ttcacgcaga ccttgtaaaa acgcgttgct ggctggcacc
    12601 acaccgcctt cgccctagat gggctcgaca atcaccgcac aggtggcgtc gtcaatcagc
    12661 gcgctggcag agttaatatc gttatatgca gcatgacgaa tatccgccgg cagtggcgca
    12721 aaatcctgtg aataggctgg ctgcccaccc gcactgacag taaacagcgt gcgaccatga
    12781 aacgcatttt tgaacgccac gatgccactc ttatggctgc cgtagcggtc gtgagcgaat
    12841 ttacgcgcca gttttagcgc cgcttcgttg gcttccgcac cggagttaca aaagaagacg
    12901 cgatcggcaa acgtggcgtc gatcaatttt ttcgccagtc gcagtaccgg ctcgttggtg
    12961 taaccgttgc cggtatgcca gaacttactc gcctgttcgt tcagcgcttc acgcagttcc
    13021 ggatgcgcat ggcccagcgc gttcaccgca atgccacccg cgaagtcgat atactctttc
    13081 ccctgctgat cccacaagcg cgaaccttcg ccacgtaccg gtataaaggg tgccggagcg
    13141 taaacaggta tcatccattc atcaaagttt tcacgcgtaa ttggctgaga catagcgacc
    13201 tctacagtaa ataattcgtt atttgtatgt taacaataag taatgtttgc gctgtaaatg
    13261 tagattgcag ggttcgtgcc agccagtgat aaaagtgcat aaacggcgga ggctaactgg
    13321 aaatcaagga gttataacca aaccatatgc atttaaagtg catataaagt gaatacgttt
    13381 gcgatgtggg tgaataaaaa gaataaaaaa cgcaatgtta tgcagaagta aaatataatt
    13441 ctagaattgt gatcattgac gaaatttact ggaaattact gcgccattct gacgcagcgc
    13501 gcaccaaaag cgggcatttt ttgcgccatc gttgacatca ttaacaacca tcgatcaaat
    13561 cacttaacaa caggcggtaa gcaacgcgaa attctgctac catccacgca ctctttatct
    13621 gaataaatgg cagcgactat gaaatttgtc tcttttaata tcaacggcct gcgcgccaga
    13681 cctcaccagc ttgaagccat cgtcgaaaag caccaaccgg atgtgattgg cctgcaggag
    13741 acaaaagttc atgacgatat gtttccgctc gaagaggtgg cgaagctcgg ctacaacgtg
    13801 ttttatcacg ggcagaaagg ccattatggc gtggcgctgc tgaccaaaga gacgccgatt
    13861 gccgtgcgtc gcggctttcc cggtgacgac gaagaggcgc agcggcggat tattatggcg
    13921 gaaatcccct cactgctggg taatgtcacc gtgatcaacg gttacttccc gcagggtgaa
    13981 agccgcgacc atccgataaa attcccggca aaagcgcagt tttatcagaa tctgcaaaac
    14041 tacctggaaa ccgaactcaa acgtgataat ccggtactga ttatgggcga tatgaatatc
    14101 agccctacag atctggatat cggcattggc gaagaaaacc gtaagcgctg gctgcgtacc
    14161 ggtaaatgct ctttcctgcc ggaagagcgc gaatggatgg acaggctgat gagctggggg
    14221 gtggtcgata ccttccgcca tgctaatccg caaacagcag atcgtttctc atggtttgat
    14281 taccgctcaa aaggttttga cgataaccgt ggtctgcgca tcgacctgct gctcgccagc
    14341 caaccgctgg cagaatgttg cgtagaaacc ggcatcgact atgaaattcg cagcatggaa
    14401 aaaccgtccg atcacgcccc cgtctgggcg accttccgcc gctaatttag cagctctcct
    14461 ggctcaaact gggtcaggag aattaacctt gagaaaaatc aacaaactgt cagtaatgat
    14521 ttgttgcctg ccgtcctttg ttataccgtc actgcgtttt tagttgtctg accacttctc
    14581 tattatcaag tttgatatag gaaactccac gatgaacgct gagcgtaact ttctgtttgc
    14641 ctgtcttatt tttgcgctgg tcatttacgc tatccacgct ttcggtttat tcgatctgct
    14701 caccgattta ccccacttac agacactcat tcgccagagc ggattgttcg gttatagcct
    14761 ctatattctg ttattcatca atgcccccct ctttctgtta ccaggaagca tactggtgat
    14821 cgctggagga atagtttttg gcccgctctt agggacacaa ctctcattaa ttgccgcaac
    14881 gctggcctcc tcgtgctcat tcctgctggc gcgctggctg ggacgtgatt tactgctgaa
    14941 atacgtgggc catagccata ccttccaggc cattgaaaaa ggcattgcgc gtaacggtat
    15001 tgattttctt attctgaccc gcttaatccc gttgtttcct tacaatattc aaaattacgc
    15061 ttacggattc accaccatcg ccttctggcc ttataccctt atttcggcac tcacgaccct
    15121 gcccggtatt gttatttata ccgtgatggc aagcgatctc gccaatgaag gcattacgct
    15181 gcgctttatt ttacaactct gtctggcggg cctggcgctg tttattctca tccagctcgc
    15241 aaaactctac gcccgacaca aacatgtgga tctgtctgct tcgcgccgca gcccacttac
    15301 tcacccaaaa aatgaaggat agaacgatgt cgcaacatta ttcagtgtca tggaaaaaag
    15361 gactggctgc actctgctta ctggctgttg ccgggcttag cggctgcgat caaaaagaga
    15421 atgcggcggc aaaagtggaa tacgacggac tttcgaacag ccaaccgctg cgcgtcgatg
    15481 ccaataacca tacggtaacc atgctggtgc aaattaatgg tcgtttcctc accgacgaca
    15541 ctcgtcacgg tattgtgttt aaagatggct ccaacggaca taaatcgctg tttatggctt
    15601 atgcgccccc gaaagcattt tatgaagccc tgaaagaggc aggtggtacg ccgggcgaaa
    15661 acatgacgat ggataataaa gaaacgactc atgtcacagg cagcaaactg gatatttcgg
    15721 tcaactggca aggggcggca aaagcgtatt ccttcgatga agtgattgtt gacagtaatg
    15781 gcaagaaact ggacatgcgc tttggcggta atttaacggc agcagaagag aagaaaacgg
    15841 gttgcctggt gtgtctggat agctgcccgg tcggcatcgt cagcaatgca acatacactt
    15901 atggtgcggt ggaaaaacac ggcgaagtga aattcaaagg caatgcctca gttctcccgg
    15961 cggataacac gctggcaacg gttaccttta aaatcaccga ataaagccaa gataaaggat
    16021 gatgaagata caatcgcgta aaatctggta ctaccgtata accctcatca tcctgttgtt
    16081 cgctatgctg ctcgcatggg cgctgcttcc cggcgtccat gagtttatca atcgcagcgt
    16141 tgcggcgttt gccgccgtgg accaacaggg tatagaacgc tttattcagt cttacggcgc
    16201 actggcggca gttgtctcgt tcttgttgat gattttacag gccattgctg caccgctacc
    16261 tgcgtttttg atcacctttg ccaatgcgtc gctgtttggc gcgttctggg gcggcttgct
    16321 gtcgtggacc agttcgatgg ccggcgcggc gctgtgcttt tttatcgcca gagtgatggg
    16381 ccgcgaagtg gtggaaaaat taaccggcaa aaccgtgctt gacagtatgg acggcttttt
    16441 cactcgctac ggcaaacaca ccatcctggt ctgtcggtta ttgccttttg tccctttcga
    16501 tccaatcagc tatgctgccg gtctgacttc aatacgtttt cgctcgtttt ttatcgccac
    16561 cgggcttggt caattaccgg cgactattgt ttattcctgg gcaggcagca tgttaacagg
    16621 cggtactttc tggtttgtca ccgaactgtt tattctgttt gccctgaccg tggtgatttt
    16681 tatggcgaag aaaatatggc ttgagcgcca gaagaggaat gactgatggg tttaccgccg
    16741 cttagcaaaa ttcctttcat tttacgtccg caggcgtggc tgcatcgtcg ccattacggc
    16801 gaggtgctaa gcccgattcg ctggtggggg cggatcccgt ttatctttta tttggtgtca
    16861 atgtttgttg ggtggctgga gcgcaaacgc tcaccgctcg atccggtagt gcgatcgctt
    16921 gtcagcgcgc gcattgcgca aatgtgcctg tgtgagtttt gcgtggatat caccagtatg
    16981 aaagtcgccg agcgtaccgg cagcaccgat aaactgctgg cagtggctga ctggcggcaa
    17041 agcccgctct ttagcgatga agaacggctg gcgctggagt acgccgaagc cgcaagcgtc
    17101 acaccgccaa cagtcgatga tgccctgcgt acccgactgg ctgcgcattt tgacgctcag
    17161 gcgctcaccg aactgacggc attgatcggc ctgcaaaacc tgtcggctcg ttttaattct
    17221 gccatggaca ttcccgctca ggggctgtgc cgtattccgg aaaaacgttc ttaaggaggg
    17281 aagatgcgcc attgtgggtg gttgctggga ttgttatcgc tgttttcttt ggcaacacat
    17341 gccagtgact ggcaggaaat taaaaatgag gccaaagggc aaaccgtctg gtttaacgcc
    17401 tggggcggcg ataccgcaat taaccgctat ctcgactggg ttagcggcga gatgaaaacc
    17461 cattacgcta taaacctgaa gattgttcgt ctcgcggatg ccgcagacgc ggtgaagcgc
    17521 attcagaccg aagctgctgc cggtcgtaaa acgggcggct cggtggatct gctctgggtg
    17581 aacggcgaaa acttccgcac cttaaaagag taatgccagt cagttaagca actgactggc
    17641 att
//
LOCUS       DAZPCS010000076        17494 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZPCS010000076 DAZPCS010000000
VERSION     DAZPCS010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17494
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.076"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..85
                     /locus_tag="ACHUQ7_002412"
     CDS             <1..85
                     /locus_tag="ACHUQ7_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1135783.1"
                     /translation="LIALARRRCDVLFAMMRDGTFYTPQGS"
     gene            111..2201
                     /gene="pbpC"
                     /locus_tag="ACHUQ7_002413"
                     /pseudo
     CDS             111..2201
                     /gene="pbpC"
                     /locus_tag="ACHUQ7_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; incomplete; partial
                     in the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
     gene            2351..2782
                     /gene="ndk"
                     /locus_tag="ACHUQ7_002414"
     CDS             2351..2782
                     /gene="ndk"
                     /locus_tag="ACHUQ7_002414"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFR1135784.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            2932..4086
                     /locus_tag="ACHUQ7_002415"
     CDS             2932..4086
                     /locus_tag="ACHUQ7_002415"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFR1135785.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPDAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            4371..5384
                     /gene="rodZ"
                     /locus_tag="ACHUQ7_002416"
     CDS             4371..5384
                     /gene="rodZ"
                     /locus_tag="ACHUQ7_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFR1135786.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            5411..6538
                     /gene="ispG"
                     /locus_tag="ACHUQ7_002417"
                     /gene_synonym="gcpE"
     CDS             5411..6538
                     /gene="ispG"
                     /locus_tag="ACHUQ7_002417"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFR1135787.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVGKIIT"
     gene            6641..7915
                     /gene="hisS"
                     /locus_tag="ACHUQ7_002418"
     CDS             6641..7915
                     /gene="hisS"
                     /locus_tag="ACHUQ7_002418"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFR1135788.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            7933..8553
                     /gene="yfgM"
                     /locus_tag="ACHUQ7_002419"
     CDS             7933..8553
                     /gene="yfgM"
                     /locus_tag="ACHUQ7_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFR1135789.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGGILGVGALTGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            8564..9742
                     /gene="bamB"
                     /locus_tag="ACHUQ7_002420"
     CDS             8564..9742
                     /gene="bamB"
                     /locus_tag="ACHUQ7_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFR1135790.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            9860..11332
                     /gene="der"
                     /locus_tag="ACHUQ7_002421"
     CDS             9860..11332
                     /gene="der"
                     /locus_tag="ACHUQ7_002421"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFR1135791.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVVNKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            11402..11617
                     /locus_tag="ACHUQ7_002422"
     CDS             11402..11617
                     /locus_tag="ACHUQ7_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFR1135792.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(11614..12984)
                     /gene="xseA"
                     /locus_tag="ACHUQ7_002423"
     CDS             complement(11614..12984)
                     /gene="xseA"
                     /locus_tag="ACHUQ7_002423"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFR1135793.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAECLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKTGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            13146..14612
                     /gene="guaB"
                     /locus_tag="ACHUQ7_002424"
     CDS             13146..14612
                     /gene="guaB"
                     /locus_tag="ACHUQ7_002424"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFR1135794.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            14681..16258
                     /gene="guaA"
                     /locus_tag="ACHUQ7_002425"
     CDS             14681..16258
                     /gene="guaA"
                     /locus_tag="ACHUQ7_002425"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFR1135795.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(16353..16892)
                     /locus_tag="ACHUQ7_002426"
     CDS             complement(16353..16892)
                     /locus_tag="ACHUQ7_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFR1135796.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16908..17426)
                     /locus_tag="ACHUQ7_002427"
     CDS             complement(16908..17426)
                     /locus_tag="ACHUQ7_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFR1135797.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQEGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
BASE COUNT         4058 a         4349 c         4856 g         4231 t
ORIGIN      
        1 gcttatcgcc ctggcgagac gacgctgcga cgttctgttc gccatgatgc gcgacgggac
       61 tttttatacc ccgcaggggt cataacatgc ttgacaactt aatagaggca cgttaccttg
      121 aagcgctgat caattatgaa gatcgctggt tctggaagca tccgggggtt aatccattct
      181 cggtggcgcg cgcagcatgg caagatctca cttcgggacg ggttatttcc ggtggcagca
      241 cgctcactat gcaggttgct cgtctgcttg atcctcaccc caaaacattt ggtggcaaaa
      301 ttctccagct ctggcgcgcg ttgcaactgg agtggcattt gtctaagcgt gaaattctga
      361 tcttgtatct taaccgcgct ccgtttggcg gtacgttgca ggggatcggt gcggcaagtt
      421 gggcttatct cggaaaatcg cctgcgaatt taagctattc cgaggcggca atgctggcgg
      481 tgttaccgca agtgcccagc cgactgcgcc cggatcgttg gccggagcgt gccgaagccg
      541 cgcgtaataa agtgctcgaa cggatggctg cgcaaggtgt gtggtcccgt gagcaggtaa
      601 aagagtcaag ggaagaaccc atctggctgg caccccgaca aatgccgcaa ctggcaccgc
      661 tgttttcgcg catgatgctc ggtaaaagca aaagcgacaa aatcgttact acgctggatg
      721 ccggtcttca acgacgtctg gaagaactgg cgcaaaactg gaaagggcgg ttgccaccgc
      781 gcagctcact ggcgatgatc gtggttaatc ataccgatat gcgtgttcgc ggctgggtgg
      841 gatcggttga tctcaacgat gattcacgct ttggtcatgt tgatatggtc aatgcgatcc
      901 gatcgccagg atcggtgctc aaaccgtttg tttatggtct ggcgctggat gaaggcttga
      961 tccacccggc atcactgctg caagacgtcc cccggcgcac cggtgattat cgaccaggta
     1021 actttgatag cggttttcat ggcccgatca acatgagcga ggcgctggtg cgctcgctga
     1081 acttacctgc tgtgcaggtg ctggaagcct atggaccgaa acggtttgcg gcaaagttac
     1141 gcaatgttgg attgccgtta tatttgccca acggcgctgc gccgaatctt tcactcattc
     1201 tcggcggcgc tggtgcaaaa ctggaagata tggcggcagc gtataccgcg tttgctcgcc
     1261 acggcaaggc aggcaaattg cgcttacagc ctgacgatcc gctgcttgaa cggcctttaa
     1321 tgtcgtcggg ggcggcgtgg atcattcgcc ggattatggc tgatgaagcg caacccttgc
     1381 cggatagtgc cttgccgcgc gtcgccccac tggcatggaa aacgggcacc agctatggct
     1441 atcgtgacgc ctgggcgatt ggggttaacg ctcgctatgt cattgggatc tggactggca
     1501 gacctgacgg cacgcccgtt gttggtcagt ttggctttgc cagtgccgta ccattgttga
     1561 atcaggtcaa taatatctta ctgtcgcgca gtgcgaatct gccggaagac ccgcgcccga
     1621 actcagtcac tcgtggcgtt atctgctggc cgggcggaca gtctttgccg gaaggtgacg
     1681 gtaactgtcg ccgccgcctg gcaacctggc tgctggatgg cagccagccg ccaacgctgt
     1741 tactgtcaga gcaggaaggg attaatggca ttcgcttccc catctggctg gatgacaaag
     1801 ggcagcgcgt cgccgctgac tgcccgcagg cgaagcaaga aatggtaaat gtctggccgc
     1861 taccgctgga accctggctg cccgcatcag aacgtcgcgc tgtgcgctta ccaccggcct
     1921 cgacaatttg cccgccatac ggtcacgatg cccagctccc tctgcagctg acaggcgttc
     1981 gcgatggcgc gattattaaa cgtttaccgg gcgcagcgga agcgactttg ccgttgcaat
     2041 caagtggagg ggcaggtgaa cgctggtggt ttctgaatgg cgaaccgtta actgaacgcg
     2101 ggcgcaacgt gaccctgcat ttgacggata aaggtgatta tcaattgctg gtgatggatg
     2161 atgtggggca aatcgcgaca gtgaaatttg tcatgcaata gtctgttttg tccctgattg
     2221 ttgctaaaac tcattttatt ttaaaaaaaa tgttaccttc atcaatagtc aacggccctg
     2281 ttgctcatta taatccgcgc catctcgtac gctggtacag acaacaacag aacaatttac
     2341 agaggtaaaa atggctattg aacgtacttt ttccatcatc aaaccgaacg cggtagcaaa
     2401 aaacgtcatt ggtaatatct ttgcgcgctt tgaagctgca gggttcaaaa ttgtcggcac
     2461 caaaatgctg catctgaccg ttgaacaggc acgtggcttt tatgctgaac acgatggaaa
     2521 accgttcttt gatggtctgg ttgaattcat gacctctggc ccgatcgtgg tttccgtgct
     2581 ggaaggtgaa aacgccgttc agcgtcaccg cgatctgctg ggcgcgacca atccggcaaa
     2641 cgcactggct ggtactctgc gcgctgatta cgctgacagc ctgaccgaaa acggtaccca
     2701 cggttctgat tccgtcgaat ctgccgctcg cgaaatcgct tatttcttcg gcgaaggcga
     2761 agtgtgcccg cgcacccgtt aataatttcg taaatgccgc gtgcaaacgt ggcatccgtg
     2821 cgccagaatt tgtacaatgc agcgcccccg gacgagcagc cgctcaccgg ggcgtttctt
     2881 ttttcaaccc tccaggggcc ataacgtgta ataacgaggc cggaatagca tatgtctgaa
     2941 caattagtca cacctgaaaa cgtcaccacg aaagatggaa aaatcaacct gctggatctc
     3001 aaccgtcagc agatgcggga gttttttaaa gatttaggtg aaaaaacctt ccgcgccgat
     3061 caggtgatga agtggatgta tcactattgc tgcgacaact ttgatgagat gaccgacatc
     3121 aacaaagtgt tgcgcggcaa actgaaagag gtggcggaaa tccgcgcacc ggaagtggtt
     3181 gaagaacagc gttcatccga cggcaccatt aaatgggcga tcgccgttgg cgatcagcgc
     3241 gtcgaaacgg tgtatatccc ggaagacgac cgtgccacgc tctgcgtctc ttcgcaggtg
     3301 gggtgtgcgc tggagtgtaa attctgttcc accgcccagc agggctttaa ccgcaacctg
     3361 cgggtatcgg aaattattgg ccaggtgtgg cgtgcggcga aaatcgtcgg cgcagcaaaa
     3421 gtcaccggtc agcgcccaat cactaacgtc gtgatgatgg gcatgggcga gccgctgctc
     3481 aacctgaaca acgtcgttcc ggcgatggaa atcatgcttg atgatttcgg ttttggcctg
     3541 tctaaacgtc gcgtgacgct ttccacttcc ggtgtagttc cggcgctgga taaactgggc
     3601 gatatgatcg acgttgcact ggcaatttcc ctgcacgcgc cgaacgacga aattcgtgac
     3661 gaaattgtgc cgatcaacaa aaagtacaat atcgaaacgt tccttgctgc ggtgcgccgt
     3721 tatctggaga aatccaacgc caatcagggc cgagtcacta ttgaatacgt gatgcttgac
     3781 cacgttaacg acggcactga acacgcgcac caactggcgg aactgctgaa agatacgccg
     3841 tgtaagatca acctgatccc gtggaacccg ttcccggacg cgccgtatgg acgcagttcg
     3901 aacagccgta tcgaccgttt ctcaaaagtg ctgatgagct acggtttcac cactattgtg
     3961 cgtaaaacgc gtggtgatga tatcgatgct gcctgtggtc agttggcggg cgatgttatc
     4021 gaccgtacga aacgtactct gcgtaaacgg atgcagggtg aagccatcga tattaaagcg
     4081 gtctgatagc cattacgcca cggttacttt atgtaccgtg gcgtaatctt ttatgaatga
     4141 agtattaagt tgcaattgtc tggtcaatat taacggtgcg tttttgctga ctttaaggca
     4201 gtatgtaacg atgcaacagt aagttagcct cagtgaatgt gggctttgtc acgagcacac
     4261 agacggtctt atactgtatg ataacggtta acttaacgga tgtttcgcgg tgtgggtgag
     4321 catgatggtt caccggcatc tcaattctca tttaaacgta cctgcagcga atgaatactg
     4381 aagccacgca cgaccaaaat gaagcactta ctaccggcgc tcgcctgcgt aatgctcgcg
     4441 aacaactagg acttagtcag caggccgttg ccgagcgact ttgcctgaag gtttccacgg
     4501 tacgcgacat tgaagaagat aaggcacccg ccgatcttgc ttcaacattc ctgcgcggat
     4561 atatccgctc ttatgcgcgt ctggtacata ttccagaaga agaactgctg ccagggctgg
     4621 aaaagcaggc tccacttcgg gctgcaaaag ttgcgccaat gcagagtttt tccctcggta
     4681 aacgccgcaa aaaacgcgac ggctggctaa tgaccttcac ctggctggtg ttgtttgtgg
     4741 ttatcggcct gagcggtgcc tggtggtggc aagaccacaa agctcagcag gaagagatca
     4801 ccactatggc cgatcaatct tcggcggaac tgagcagtaa tagcgagcag gggcagagtg
     4861 ttccgttaaa tacgtcgaca actacagacc cggctacaac gtccacgccg ccagcgtctg
     4921 tggatactac cgcaaccaac acgcaaacac ctgccgtaac tgcgccagca ccagctgttg
     4981 atccgcaaca gaatgcggtt gtttcgcctt cgcaggcaaa tgttgatacc gccgcgaccc
     5041 cggtaccaac ggcagcaaca acgccagatg gtgctgcgcc gttgccaacc gatcaggctg
     5101 gcgtgaccac gccggcggct gatccgaatg cgctggtgat gaactttact gccgattgct
     5161 ggctggaggt cactgatgct accggtaaaa aattgtttag cgggatgcag cgtaaagacg
     5221 gtaatttgaa tttaaccggc caggcaccgt ataaactgaa aattggtgcg ccagccgcag
     5281 tacagatcca gtatcaaggg aaacctgtcg atctgagtcg ttttatcaga actaaccagg
     5341 ttgcgcgtct gaccctcaat gccgaacaat caccggcgca gtaacagacg ggtaacgcgg
     5401 gagatttttc atgcataacc aggctccaat tcaacgtaga aaatcaacac gtatttacgt
     5461 tgggaatgtg ccgattggcg atggtgctcc catcgccgta cagtccatga ccaatacgcg
     5521 tacgacagat gtcgaagcaa cggtcaatca aatcaaggcg ctggaacgcg ttggcgctga
     5581 tatcgtccgt gtctccgtac cgacgatgga cgcggcagaa gcgttcaaac tcatcaaaca
     5641 gcgggttaac gtgccgctgg tggctgacat ccacttcgac tatcgcattg cgctgaaagt
     5701 agcggaatac ggcgtcgatt gtctgcgtat taaccctggt aatatcggta atgaagagcg
     5761 tattcgcatg gtggttgact gtgcgcgcga taaaaacatt ccgatccgta ttggcgttaa
     5821 cgccggatcg ctggaaaaag atctgcaaga aaagtatggc gaaccgacgc cgcaggcgtt
     5881 gctggaatcc gccatgcgtc atgttgatca tctcgatcgc ctgaacttcg atcagttcaa
     5941 agtcagcgtg aaagcgtctg acgtcttcct cgctgttgag tcttatcgtt tgctggcaaa
     6001 acagatcgat cagccgctgc atctggggat caccgaagcc ggtggtgcac gcagcggggc
     6061 agtaaaatcc gccattggtt taggtctgct gctgtcggaa ggcatcggcg acacgctgcg
     6121 tgtatcgctg gcggccgatc cggtcgaaga gatcaaagtc ggtttcgata ttttgaaatc
     6181 gctgcgtatc cgttcgcgag ggattaactt catcgcctgc ccgacctgtt cgcgtcagga
     6241 atttgatgtt atcggtacgg ttaacgcgct ggagcaacgc ctggaagata tcatcactcc
     6301 gatggacgtt tcgattatcg gctgcgtggt gaatggccca ggtgaggcgc tggtttctac
     6361 actcggcgtc accggcggca acaagaaaag cggcctctat gaagatggcg tgcgcaaaga
     6421 ccgtctggac aacaacgata tgatcgacca gctggaagca cgcattcgtg cgaaagccag
     6481 tcagctggac gaagcgcgtc gaattgacgt tcagcaggtt ggaaaaataa taacgtgatg
     6541 ggaagcgcct cgcttcccgt gtatgattga acccgcatgg ctcccgaaac attgagggaa
     6601 gcgttgaggg ttcattttta tattcagaaa gagaataaac gtggcaaaaa acattcaagc
     6661 cattcgcggc atgaacgatt acctgcctgg cgaaacggcc atctggcagc gcattgaagg
     6721 cacactgaaa aacgtgctcg gcagctacgg ttacagtgaa atccgcttgc cgattgtaga
     6781 gcagaccccg ctattcaaac gtgcgattgg tgaagtcacc gacgtggttg aaaaagagat
     6841 gtacaccttt gaggatcgca atggcgacag cctgactctg cgccctgaag ggacggcggg
     6901 ctgtgtacgc gccggcatcg agcatggtct tctgtacaat caggaacagc gtctgtggta
     6961 tatcgggccg atgttccgtc acgagcgtcc gcagaaaggg cgttatcgtc agttccatca
     7021 gttgggctgc gaagttttcg gtctgcaagg tccggatatc gacgctgaac tgattatgct
     7081 cactgcccgc tggtggcgtg cgctgggtat cttcgaacac gtaactcttg agctgaattc
     7141 tatcggttcg ctggaagcac gcgccaatta ccgcgatgcg ctggtggcat tccttgagca
     7201 gcataaagaa aagctggacg aagactgcaa acgccgcatg tacactaacc cgctgcgcgt
     7261 gctggattca aaaaatccgg aagtgcaggc gcttctcaac gacgctccgg cattaggtga
     7321 ttatctggac gaggaatctc gtgagcattt tgccggtctg tgcaaactgc tggagagcgc
     7381 ggggatcgct tacaccgtaa accagcgtct ggtgcgtggt ctggattact ataaccgtac
     7441 cgttttcgag tgggtgacta acagtctcgg ctcccagggc accgtgtgtg caggcggtcg
     7501 ttatgacggt cttgtggaac aactgggcgg tcgtgcaaca ccggctgtcg gttttgcgat
     7561 gggcctcgaa cgtcttgtat tgttagtaca ggccgttaat ccggaattta aagccgatcc
     7621 tgttgtcgat atatacctgg tggcttcagg tgctgataca caatctgcgg ctatggcatt
     7681 agctgagcgt ctgcgtgatg aattactggg cgtgaaattg atgaccaacc acggcggcgg
     7741 caactttaag aaacagtttg cccgtgctga taaatggggt gcccgcgttg ctgtggtgct
     7801 gggtgagtct gaagtggcta acggcacagc agtagtgaag gatttgcgct ctggtgagca
     7861 aacggcagtt gcgcaggata gcgtagccgc gcatttgcgc acgttactgg gttaaggaag
     7921 gagaaggaca gcgtggaaat ttacgagaac gaaaacgacc aggtagaagc ggttaaacgc
     7981 ttttttgctg aaaatggcaa agcactggct gttgggggga ttttgggcgt tggcgcactg
     8041 actggctggc gctactggaa cagccatcag gttgattctg cacgctccgc ttctcttgcc
     8101 tatcaaaatg cggttaccgc agtgagcgaa ggcaaaccgg atagcatccc ggcggcggaa
     8161 aaatttgctg ctgaaaataa aaatacttat ggtgcgctgg cttctttgga acttgcgcag
     8221 caatttgttg acaaaaatga actagagaaa gctgccgccc agttacaaca ggggctggca
     8281 gacacgagcg atgaaaatct caaagccgtg ataaatctgc gtcttgctcg cgttcaggta
     8341 cagctcaagc aggctgatgc cgcgctgaaa acccttgata ccatcaaagg tgaagggtgg
     8401 gctgccattg ttgccgactt gcgtggtgaa gcattgctga gcaaaggtga taagcaaggt
     8461 gcgcgtagtg catgggaagc aggcgtgaaa agcgatgtta ctccggcact gagcgaaatg
     8521 atgcagatga aaattaataa tttgtccatc tgagagggac ccgatgcaat tgcgtaaatt
     8581 actgctgcca ggactgcttt ccgttaccct tttaagcggc tgttcgctgt ttaacagcga
     8641 agaagatgtg gtaaagatgt ccccattgcc aaccgttgaa aaccagttta cgccgaccac
     8701 ggcgtggagc acttccgttg gtagcggcat tggcaacttc tattccaatc ttcatccggc
     8761 actggcggac aacgttgtct atgcagcgga ccgcgctggt ttggtaaaag cgctgaatgc
     8821 ggatgatggc aaagaaatct ggtctgtcag cctggccgag aaagatggct ggttctctaa
     8881 agagcctgca ttactttctg gcggtgtgac cgtgtctggt gggcatgtct acattggcag
     8941 cgaaaaggcg caggtttacg cgctgaatac cagcgatggt actgtggcat ggcaaactaa
     9001 agtcgcgggt gaagcacttt cgcgcccggt ggtcagcgac ggtctggtgt taatccacac
     9061 cagtaacggt cagttacaag cgctgaacga agctgacggc gctgtcaaat ggacagttaa
     9121 cctcgatatg ccttcgctct ctttgcgtgg cgagtctgcg ccggcaacgg cttttggtgc
     9181 ggccgtcgtg ggtggcgata atggtcgcgt cagcgcagtg ctgatggaac agggccagat
     9241 gatttggcag cagcgtattt cccaggcgac cggttctacc gaaattgacc gtctgagcga
     9301 tgttgacacg actcccgtcg ttgttaacgg cgttgttttc gcgctggcct ataatggtaa
     9361 cctgacggcg cttgatctgc gcagtggtca gattatgtgg aaacgcgaac tgggttcggt
     9421 gaatgatttc atcgtcgatg gcaatcgcat ctatctggtc gatcaaaatg accgggtgat
     9481 ggcgttgacc attgatggcg gcgttacgct gtgggcacaa agcgatctgc tgcatcgcct
     9541 gctgacttct ccggtgctgt ataatggcaa cctggtggtc ggtgacagtg aaggttatct
     9601 gcactggatt aacgtcgaag atggtcgttt cgttgcccag caaaaagttg atagttccgg
     9661 tttccagact gaaccggttg ccgctgacgg caaactgctg atccaggcaa aagacggaac
     9721 cgtgtactct attacacgtt aatcgtctct gtcgttcact ttgaaaacgg ctcctggaca
     9781 ggggccgttt tcctgttttt aacaacgacg cgaatatagc gtgcgttgtc tgatgattta
     9841 taaaaatgag gctttaaaca tggtacctgt ggtcgcgctt gtcgggcgcc ctaacgtagg
     9901 aaaatccacg ttatttaacc gtctaactcg cacccgagat gcgctggttg cggatttccc
     9961 gggtctgact cgtgaccgta agtacggtcg tgcggaaatt gaaggccgtg agtttatctg
    10021 tattgatacc ggcgggattg atggcacaga agacggtgta gaaacccgca tggcggaaca
    10081 gtcgctgctg gcgattgaag aagcggacgt cgtactgttt atggtggatg cgcgcgcggg
    10141 cctgatgccg gcagatgaag cgattgccaa acatctgcgc tcccgtgaaa aaccgacctt
    10201 cctggtggta aacaaaactg acggtctgga tcccgatcag gcagtggttg atttctactc
    10261 gcttggttta ggtgaaatct acccgatcgc cgcgtctcac ggtcgtggcg tattaagtct
    10321 gctggagcat gtgctgctgc cgtggatgga agatctcgca ccgcaagagg aagtcgacga
    10381 agacgctgaa tactgggcgc aatttgaagc ggaagagaac ggcgaagaag aagaggaaga
    10441 cgacttcgac ccgcaaagtc tgccgatcaa actggcgatt gtgggtcgtc cgaacgtagg
    10501 taagtctaca ctcactaacc gtattcttgg agaagagcgc gttgttgttt acgacatgcc
    10561 tggcacgacg cgtgacagca tctacatccc aatggaacgc gatggacgtg agtatgtgct
    10621 cattgacacc gctggcgtac gtaaacgcgg caaaatcacc gatgctgtag agaaattctc
    10681 cgtaatcaaa acgttgcagg ccattgaaga cgccaacgtg gtgatgttag tgattgatgc
    10741 gcgcgaaggt atttccgatc aggatctctc gctgctgggc tttattctca atagtgggcg
    10801 ctcacttgtc attgtggtga ataagtggga tggcctgagc caggaagtga aagagcaggt
    10861 gaaagaaacg ctggacttcc gtctgggctt tatcgatttt gctcgtgtgc actttatctc
    10921 tgccttgcac ggcagtggtg ttggtaactt gtttgaatca gtacgtgaag cgtatgacag
    10981 ctccacccgt cgtgtgggga cctctatgct gacgcgcatc atgacgatgg ctgttgaaga
    11041 tcaccaaccg ccgctggtac gcggtcgtcg tgtgaagctg aaatatgccc acgctggtgg
    11101 ttataacccg ccgattgtgg tgattcacgg taatcaggtg aaagacctgc ctgattccta
    11161 caagcgctac ttgatgaact acttccgcaa atcgctggac gtaatgggat cgccgattcg
    11221 tattcagttc aaagaagggg aaaacccgta tgcgaacaag cgtaacaccc tgacgccaac
    11281 ccagatgcgt aaacgtaagc gtctgatgaa gcacatcaag aaaaataaat aattatccgt
    11341 aagtatttta tctgccattc cctctatgaa tgtagaggga atggctaccg gaggaattca
    11401 tatggaactg cattgtccaa aatgtcagca tgtgcttgat caggataacg gccatgcgcg
    11461 ttgcccatcc tgcggggaat ttattgaaat gaaagctctt tgtccagact gccatcaacc
    11521 gttgcaggtg ttaaaagcct gcggtgcagt agattacttc tgtcagcacg ggcatggctt
    11581 gatatcgaaa aaacgcgttg aattcgtgct ggcttaatga acctttttac gcgatttttt
    11641 taccggctgg atgtttttta cttcactttc tatccagccg tcttccagac gtgtggttag
    11701 catttcaccc gttttaactt gcttcacttt tttcagtaca ttgccgtcag tagcagtagt
    11761 aacgctgtat ccacgcgcca gcgttgacag tgggcttacg gcttcgaggt gcgttactgc
    11821 attaccgaaa cgttcacgcg tggcgctaag ctgtgcgcgc aggatttctg ctaaacgata
    11881 ttccagttgc tgaatgcgcg tttgcgtgcg atgaatcttc ggctgtggat tttgctgatt
    11941 cagccgctgt gttaatcgct gctgctgttg cccggcacgc ttaagctgat tttccagcgc
    12001 aaagctcatc cgtttttgca ggcgttcaag catggtttgc tggcgtgcca gccggagctg
    12061 cggatgctgc tgctgcaagc gatgatgaat ctgcgtaaag cgacgtgtac ggttggcgag
    12121 ataataatcc atcgccatct ccagccgttg acgggccgat tgcacctggc gcagtaactc
    12181 ttgctgatta cggctcacta cttcagcggc ggcagacggc gttggcgcac gcagatcggc
    12241 aacaaaatcg gcaatggtca catccgtctc atgcccgacg gcgctgacga ccggaatgcg
    12301 gctggcaaaa atcgcccgcg ctacgcgttc gtcgttaaaa ctccataaat cttccagcga
    12361 accgccgccg cgcccaacga tcaacacgtc gcactcattg cgctgattcg ccagttcaat
    12421 ggcgcgaaca atttgccccg gcgcgtcatc gccctgaacg gaggtggggt agatgatcac
    12481 cggtagagaa ggatcgcgac gttttaacac atgcaaaata tcatgtagcg cagcaccggt
    12541 ttttgaggtg atcacaccaa cgcaatgcgc aggggaggga agtggttttt tgtattgctg
    12601 atcgaacaaa cattcagcct gcaacttcgc tttgagctgt tcgtacttct gttgcagcag
    12661 cccttcaccg gccggctgca tactctcaac gattatctgg tagtcgccgc gcggctcgta
    12721 gagcgtaata ttggcgcgaa ctaaaacttg ttgcccatgc tgcggacgga aggtgacccg
    12781 gcggttgctg ttgcggaaca tcgcgcagcg tacctgggcg gtgtcgtctt tgagtgtaaa
    12841 gtaccagtga ccggaagccg gttgtgtgaa attagagatt tcgccgctga tccaaacctg
    12901 tcccatctca tgctcaagca gcagacgaac cgtttgattc aggcgactaa cggtaaaaat
    12961 tgcaggggat tgagaaggta acatgtgagc gagatcaaat tctaaatcag caggttattc
    13021 agtctatagt aacccgccct tcggggatag caagcatttt ttgcaaaaag gggtagatgc
    13081 aatcggttac gctctgtata atgccgcggc aatatttatt aaccactctg gtcgagatat
    13141 tgcccatgct acgtatcgct aaagaagctc tgacgtttga cgacgttctc ctcgttcctg
    13201 ctcattctac cgttctgccg aatactgctg acctcagcac ccagctgacg aaaactattc
    13261 gtctgaatat ccctatgctt tccgcagcaa tggataccgt aacggaagcg cgcctggcta
    13321 ttgctctggc tcaggaaggc ggtatcggct ttatccacaa aaacatgtcc attgaacgcc
    13381 aggcagaaga agttcgccgt gtgaaaaaac acgaatctgg tgtggtgact gatccgcaga
    13441 ctgttctgcc aaccacgacg ctgcgcgaag tgaaagaact gaccgagcgt aacggttttg
    13501 cgggctatcc ggtcgttacc gaagaaaacg aactggtggg tattatcacc ggtcgtgacg
    13561 tgcgttttgt taccgacctg aaccagccgg ttagcgttta catgacgccg aaagagcgtc
    13621 tggtcaccgt gcgtgaaggt gaagcccgtg aagtggtgct ggcaaaaatg cacgaaaaac
    13681 gcgttgaaaa agcgctggtg gttgatgacg aattccacct gatcggcatg atcaccgtga
    13741 aagacttcca gaaagcggaa cgtaaaccga acgcttgtaa agacgagcaa ggccgtctgc
    13801 gtgttggtgc tgcagttggc gcaggtgcgg gtaacgaaga gcgtgttgac gcgctggttg
    13861 ccgctggcgt tgacgttctg ctgatcgact cctcccacgg tcactcagaa ggcgttttgc
    13921 agcgtatccg tgaaacccgt gctaaatatc cggatctgca aatcatcggc ggcaacgtgg
    13981 caacagctgc aggtgcacgc gctttggcag aagctggttg cagcgcggtt aaagtcggta
    14041 tcggccctgg ttctatctgt acgactcgta tcgttactgg cgtaggtgtt ccgcagatca
    14101 ctgccgttgc tgacgcagtt gaagccctgg aaggcaccgg tattccggtt atcgcagatg
    14161 gtggtattcg cttctccggc gacatcgcca aagctatcgc cgctggcgca agcgcggtga
    14221 tggtaggttc catgctggcg ggtactgaag aatctccggg tgaaatcgaa ctctaccagg
    14281 gccgttctta taaatcttac cgtggtatgg gttccctggg cgcgatgtcc aaaggttcct
    14341 ctgaccgtta tttccagagc gataacgctg ccgacaaact ggtgccggaa ggtatcgaag
    14401 gtcgcgtagc ttataaaggt cgcctgaaag agatcattca ccagcagatg ggcggcctgc
    14461 gctcctgtat gggtctgacc ggctgtggta ctatcgacga actgcgtact aaagcggagt
    14521 ttgtacgtat cagcggtgcg ggcattcagg aaagccacgt tcacgacgtg accattacta
    14581 aagagtcccc gaactaccgt ctgggctcct gattctcttc gcccgatttc atgtcgggcg
    14641 atttatatta tctgtttcac ttgcctcgga ataagcgtca atgacggaaa acattcataa
    14701 gcatcgcatc ctcattctgg acttcggttc tcagtacact caactggttg cgcgccgcgt
    14761 gcgtgagctg ggtgtttact gcgaactgtg ggcgtgggat gtgacagaag cacaaattcg
    14821 tgacttcaat ccaagcggaa ttattctttc cggcggcccg gaaagtacta ctgaagaaaa
    14881 cagtccgcgt gcgccgcagt atgtctttga agcaggcgta ccgatattcg gcgtttgcta
    14941 tggcatgcag accatggcaa tgcagttggg cggtcacgtt gaagcctcta acgaacgtga
    15001 atttggctac gcgcaggtag aagtcgtaaa cgacagcgca ctggttcgcg gtatcgaaga
    15061 tgcgctgacc gcagacggta aaccgctgct cgatgtctgg atgagccacg gcgataaagt
    15121 taccgctatc ccgtccgact tcgtcaccgt agccagcacc gaaagctgcc cgtttgccat
    15181 tatggctaac gaagaaaaac gcttctatgg cgtacagttc cacccggaag tgactcacac
    15241 ccgccagggt atgcgcatgc tggagcgttt tgtgcgtgat atctgccagt gtgaagccct
    15301 gtggacgcca gcgaaaatta tcgacgatgc tgtagctcgc atccgcgagc aggtaggcga
    15361 cgataaagtc atcctcggcc tctctggtgg tgtggattcc tccgtaaccg caatgctgct
    15421 gcaccgtgct atcggtaaaa acctgacttg cgtattcgtc gacaacggcc tgctgcgtct
    15481 caacgaagca gagcaggttc tggatatgtt tggcgatcac tttggtctta acattgttca
    15541 cgtaccggca gaagatcgct tcctgtcagc gctggctggc gaaaacgatc cggaagcaaa
    15601 acgtaaaatc atcgggcgcg ttttcgttga agtattcgat gaagaagcgc tgaaactgga
    15661 agacgtgaag tggctggcgc agggcaccat ctaccctgac gttatcgaat ctgcggcttc
    15721 tgcaaccggt aaagcacacg tcatcaaatc tcaccacaac gtgggcggcc tgccgaaaga
    15781 gatgaagatg ggcctggttg aaccgctgaa agagctgttc aaagacgaag tgcgtaagat
    15841 tggtctggag ctgggcctgc cgtacgacat gctgtaccgt catccgttcc cgggaccagg
    15901 ccttggcgtt cgtgttctgg gtgaagtgaa gaaagagtac tgtgacctgc tgcgccgtgc
    15961 tgacgccatc ttcattgaag aactgcgtaa agcggacctg tacgacaaag tcagccaggc
    16021 gttcaccgtc ttcctgccag tacgttccgt tggcgtaatg ggcgatggtc gtaagtatga
    16081 ctgggttgtc tctctgcgtg ctgtcgaaac catcgacttt atgaccgcac actgggcgca
    16141 tctgccgtac gatttcctcg gtcgcgtttc caaccgcatt atcaatgaag tgaacggtat
    16201 ttcccgcgtg gtgtatgaca tcagcggcaa gccgccagct accattgagt gggaatgatt
    16261 ttgatccctg cactatgaat gaacaaaacc ctccgttact acagagggtt ttttatcttc
    16321 aagaatcata ggcttgaagt tactaacatc gattaattaa accagctgtc cgatttgttc
    16381 tcttctgctt tgcccacgct tttcatcaga tcgcgaccgc cttcagtcat atttctgttt
    16441 gcgtcagctt cagattgcac cacatcggtt tgcgcagctt tgtgcttcag ttcctgatcg
    16501 ataaattcgt tttcgcgctt aacgcgggct tcttctttcg ccagcgccag tttttgtttc
    16561 tgaatctcta agctgcgtag ctcatcttca taactttgat cgcgtttttt gtccgcagtg
    16621 gcttcggcgt ccagtttatc ctgacgagct ttcttatttg ccgctgccgt tgccgctctt
    16681 ttgttcgcgg cggcctgggc atttgcgcga cgttgcttct cttgctggat ttccctgttg
    16741 cgctccgcga cccattcgtc atgctgcctt tgctcttcat ttttaccttg ctgttccgct
    16801 tctgcgacag cagagagttg atcctgcaat gatgaggcta tagccggata gcttaaggag
    16861 gctaagatgg cgcaaagaaa aactttcttc atgactcctc ctgattatta gctcttttca
    16921 ggacatttgg tatttggctg aatacgcgtt tcgttatacg ttgtggtaat aacaacggct
    16981 aaacctgtcg taaactggca ctctttaccc tcctgggtgg aggtatacac tttggtgcct
    17041 tccttatatg ttaaagaaac accttccact aaggttttat cattcaccat agaacccgct
    17101 gccgcgccta cagctccgcc gccaactgcc cctgccgtcg ttccggaatt gctgccagaa
    17161 ccgacgttgt ggccgataac accgccagcg actgcgccaa taagcgcgcc gaaggcttgt
    17221 gcgttccgtt tattttggga gttgtctacg gcaacttttg cgggaagaat ggaaataata
    17281 ttaacggttt tagtttcttg tttggtattc agttgatcgg tttgataaac atcggcggca
    17341 tgatcgtcag catttgactg gcatcctgcc agagtgaatg acgctaacat tgccacaggc
    17401 agaagacatt ttttaaattt catcactatt ccttgttata cacattacga aaaataagac
    17461 atttaagatc atcaaaagag gaaagtaaat gtat
//
LOCUS       DAZPCS010000077        17403 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZPCS010000077 DAZPCS010000000
VERSION     DAZPCS010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17403
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.077"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(42..773)
                     /gene="rlmB"
                     /locus_tag="ACHUQ7_002428"
     CDS             complement(42..773)
                     /gene="rlmB"
                     /locus_tag="ACHUQ7_002428"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFR1135798.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(953..3394)
                     /gene="rnr"
                     /locus_tag="ACHUQ7_002429"
     CDS             complement(953..3394)
                     /gene="rnr"
                     /locus_tag="ACHUQ7_002429"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFR1135799.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRCQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3433..3858)
                     /gene="nsrR"
                     /locus_tag="ACHUQ7_002430"
     CDS             complement(3433..3858)
                     /gene="nsrR"
                     /locus_tag="ACHUQ7_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFR1135800.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4063..5361)
                     /gene="purA"
                     /locus_tag="ACHUQ7_002431"
     CDS             complement(4063..5361)
                     /gene="purA"
                     /locus_tag="ACHUQ7_002431"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFR1135801.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5465..5662)
                     /locus_tag="ACHUQ7_002432"
     CDS             complement(5465..5662)
                     /locus_tag="ACHUQ7_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFR1135802.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5744..6748)
                     /gene="hflC"
                     /locus_tag="ACHUQ7_002433"
     CDS             complement(5744..6748)
                     /gene="hflC"
                     /locus_tag="ACHUQ7_002433"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFR1135803.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6751..8010)
                     /gene="hflK"
                     /locus_tag="ACHUQ7_002434"
     CDS             complement(6751..8010)
                     /gene="hflK"
                     /locus_tag="ACHUQ7_002434"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFR1135804.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYSNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8096..9376)
                     /gene="hflX"
                     /locus_tag="ACHUQ7_002435"
     CDS             complement(8096..9376)
                     /gene="hflX"
                     /locus_tag="ACHUQ7_002435"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFR1135805.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKAMGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9452..9760)
                     /gene="hfq"
                     /locus_tag="ACHUQ7_002436"
     CDS             complement(9452..9760)
                     /gene="hfq"
                     /locus_tag="ACHUQ7_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFR1135806.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9846..10796)
                     /gene="miaA"
                     /locus_tag="ACHUQ7_002437"
     CDS             complement(9846..10796)
                     /gene="miaA"
                     /locus_tag="ACHUQ7_002437"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFR1135807.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSV
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10789..12636)
                     /gene="mutL"
                     /locus_tag="ACHUQ7_002438"
     CDS             complement(10789..12636)
                     /gene="mutL"
                     /locus_tag="ACHUQ7_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFR1135808.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12646..13983)
                     /gene="amiB"
                     /locus_tag="ACHUQ7_002439"
     CDS             complement(12646..13983)
                     /gene="amiB"
                     /locus_tag="ACHUQ7_002439"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFR1135809.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGMIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVAHKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKLSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14002..14463)
                     /gene="tsaE"
                     /locus_tag="ACHUQ7_002440"
     CDS             complement(14002..14463)
                     /gene="tsaE"
                     /locus_tag="ACHUQ7_002440"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFR1135810.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14435..15982)
                     /gene="nnr"
                     /locus_tag="ACHUQ7_002441"
     CDS             complement(14435..15982)
                     /gene="nnr"
                     /locus_tag="ACHUQ7_002441"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFR1135811.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPSEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15981..17120
                     /gene="queG"
                     /locus_tag="ACHUQ7_002442"
     CDS             15981..17120
                     /gene="queG"
                     /locus_tag="ACHUQ7_002442"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFR1135812.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGALPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17103..17156)
                     /locus_tag="ACHUQ7_002443"
     CDS             complement(17103..17156)
                     /locus_tag="ACHUQ7_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1135813.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3948 a         4961 c         4374 g         4120 t
ORIGIN      
        1 aaaccatcca aatctggatg gcttttcata attcagagga actagctgcg ctgacgaacc
       61 gcttcaaata agcaaattcc ggttgcaacc gaaacgttca gggaagaaac acttcctgcc
      121 attgggatgc tgatcaactc atcgcaatgt tcacgggtca ggcgacgcat accttcacct
      181 tccgcgccca tcaccagcgc caggcgtccg gtcattttgc tctgatagag tgtatgatcc
      241 gcctcgcctg ccgtaccgac gatccagata ttctcttcct gcaacatacg catggtgcgc
      301 gcaaggttag tcacccgaat cagcggaacg ctttctgccg cgccgcaggc tacttttttc
      361 gccgtagcgt tgagctgtgc ggagcgatct ttcggcacaa tcaccgcatg aacgcctgcg
      421 gcgtccgcac tacgcaggca cgcgccgagg ttgtgcggat cggttacgcc gtcgaggatc
      481 agcaggaacg gttgatcgag cgaagcgatc agatccggca gatcgttttc ctgatactga
      541 cgtcctggct tcacgcgggc gataatgccc tgatgcacgg caccgtcgct tttctcgtcg
      601 agatattggc ggtttgccaa ctggataacc acgccctgag actcaagggc gtgaatcagc
      661 ggtaacagac gtttatcttc acggcctttt aaaataaaga cttcctgaaa acgttcaggg
      721 gcgcgctcca gcagggcctg cactgcgtgg atgccgtaaa tcatttcgct cattaatgta
      781 ctcgttgttt acgttttgcc tgatgcacta cgtttatccg gcctacatga tctctgcaat
      841 atattgaatt tgcgtgatta tgtaggccgg ataaggcgtt cacgccgcat ccggcatgaa
      901 caaagcgcat tttgtcagca atctaaccct cttcttttaa agagggtatt gatcactctg
      961 ccactttttt cttcgccgca cgcttcgctt tggtcgctgc ggctattttc tgcgttttcg
     1021 ccgatggctt tttcgctttt ctcgcgtctt tcttcgccgc tttcggcttc gtttttttct
     1081 caccgcggaa ggcgctgtct ggctcaaagt ttaccttttt accgacctga caacgcttgc
     1141 cgcctttttt gcctgcatcg ccttttttcg ctttctcgcg cgccgtttta ccgacgttgc
     1201 gcggtgcgcg ttcactggag atcagactaa agtcgatttt gcgctcgtcc atattaaccg
     1261 cttcgacgcg aacttccacg cgatcgccca ggcgataagt ctggccgctg gattccccca
     1321 tcaggcgttg ccctacctgg tcaaagcgat agtagtcatt gtccagcgaa gagacatgga
     1381 ccagaccatc aatgaacaag tcgtccagac ggacgaagaa gccaaagcca gtgacgctgg
     1441 aaattacgcc tttaaagacg ttacctacct ggtcgagcat gaagtcacac ttcagccagt
     1501 cagcgacatc gcgcgttgct tcgtcggcac gacgttccgc catcgaacag tgctgaccca
     1561 gttgcaacat ctcttccatc gaataatggt agccgccggt ttcagtggtg ttgccctgat
     1621 gcccctgctc tttcgccagc agatatttaa tggcgcggtg cagcgtcagg tcaggataac
     1681 gacgaatcgg cgaagtaaag tgcgcatagg actgcaatgc cagaccgaag tgaccacggt
     1741 tttctggatc gtaaatcgcc tgtttcatcg agcgtagcag catggtttgc agcatttctg
     1801 catcaggacg gtcggcaacc gactccagca gttccgcgta gtcacgcggt tccggcttgt
     1861 taccacccgg cagctccagc cccagctccg ccagcactga acggaaagag gtaatcgctt
     1921 cggtgctcgg cttgtcgtga atacggaaca gtgccggttc tttcgctttc tcaacgaaac
     1981 gcgccgccga gatattcgcc agaatcatgc actcttcaat taatttgtgc gcgtcgttac
     2041 gctgggtctg ttcgatacgt tcaatacggc gttcagcgtt gaaaatgaac ttcgcttctt
     2101 cgctctcaaa tgagatccca ccgcgttctt cacgggcttt atccagcact ttatagaggt
     2161 tatgcaactc ttcgagatgc ttaaccagcg gggcgtactg ctcgcgcaga tcctgatcgc
     2221 cctgcagaat atgccagact ttggtgtagg tcagacgcgc gtgagagctc atcaccgctt
     2281 cgtagaattt gtagcccgtc aggcggcctt tcgacgaaac cgtcatctcg cacaccatac
     2341 acaggcggtc tacctgcggg ttgagcgaac acaggccgtt agagagcact tccggcagca
     2401 tcgggataac ctgcgaaggg aagtacaccg acgtgccacg gttacgcgct tctctgtcca
     2461 gcggcgttgg cggacgcaca tagtagctga cgtcggcaat cgcgacccat aaacgccagc
     2521 cgccgccgcg ttttttctcg cagtaaactg catcgtcaaa gtcacgggcg tcttcgccat
     2581 caatggtgac cagcggtaaa tcgcgcaaat cgacacggcc cgcttttgct tcttccggca
     2641 cttcttcttt cagccccgca acctgttgct caacagcctg cggccagata tacggaattt
     2701 catgggtacg cagagcgata tcaaccgcca tgccggtgcc catattgtcg cccagcactt
     2761 cgacgatttt acccaccgct ttggtgcggc gagtcggacg ctgagtcagt tcgactacga
     2821 ccacaaagcc catccgcgcg cccatgatct gatcgggcgg gattaagata tcgaagctca
     2881 gacggctatc gtcaggaacc acaaagccga cgcccgcttc ggtaaagtag cgaccaacaa
     2941 tctggctggt ttttggcacc agtacgcgga caatacgcgc ttcacgacga cctttacggt
     3001 cagcacccag cggctgcgcc agcacctgat cgccatgaat gcaggttttc atctgctcgc
     3061 tggagagata caaatcatct ttacgccctt caacccgcag aaagccgtag ccatcacggt
     3121 gaccaataac ggtacctttc accaggtcga ggcgttccgg cagcgcatag cactgacgac
     3181 gagtgaagac cagttgacca tcgcgctcca tcgcgcgcag gcggcgacgc aggccttcaa
     3241 gctgctcttc gccttcaatg tgcagttcta ccgccagctc atcacggctg gccggttttt
     3301 cacgtttggt taaatgttcg aggataaatt cccgactagg gatgggattc gcgtattttt
     3361 cagcttcgcg ttcctggaaa ggatcttgtg acatctcggt tcctccgttg tcatctctga
     3421 tgaagatttt cgtcactcca ccagcaataa tttataaagc ggttgattct cttcaaccaa
     3481 atcggcaagc gtgtagttat ccagttccgt aagaaaactt tgcacggcct tagaaagcgc
     3541 ctgtttcaac cgacaggcag gtgtaatgtg gcaaaactca ctgctgcaat tcaccagcga
     3601 taagggctcc agctcgcgca ccacatcacc aatacgtatc gcactcgccg gtttacccag
     3661 gcgaatgccg ccattttttc cacgaacagc agtcacgtag ccggcacgac taagttgatt
     3721 gattattttg accatatgat tacgggagac gccgtagacg tcagtcactt cagaaatact
     3781 ggtcatccgc ccttctggca atgacgccat gtagatcagc gcacgtaatc cgtaatcagt
     3841 gaaactcgtt aactgcacat caacctcaaa agggaaatcg ggaaaaaata catttatatt
     3901 gatgataaac cagccacaag ctgtgtcgct aatttatttc agttcgggga aggaaaaaag
     3961 cgaggattta acactgtgtc ggatagcgta atggcttaac cgacacagca atgtatcaca
     4021 ctgttagccc ggcaggcaaa atatctgcca ggcgtaccag aattacgcgt cgaacgggtc
     4081 gcgcagaatc atggtttcag tacgatccgg accggtagag atgatatcga tcggcacacc
     4141 ggtcagctct tcaatacgct tgatgtagtt cagcgccgcc tgcggcaggc cgctacgatc
     4201 tttcacgccg aaagtggatt cagaccagcc cggcatggtt tcgtaaatcg gctctacacc
     4261 tttccagtcg tcagctgcca gcggagtggt agtcacttcg cgaccatccg gcatacggta
     4321 agccacgcag agtttaacct ctttcaggcc atccagaacg tccagtttag tcaggcagaa
     4381 gccagacagg gagttcagct gtaccgcacg acgaacggca acggtgtcca gccagccggt
     4441 acgacgacga cgacccgtag ttgcaccgaa ttcgttaccc tgcttgcaga ggaactcgcc
     4501 agtttcatca aacagttcgg ccgggaaagg acctgcacct acacgagttg agtaagcttt
     4561 gaggataccc agaacgtaat caacataacg cgggcccagg ccggaaccgg tcgccacgcc
     4621 accagcagtg gtgttggaag aagttacgta cggataagta ccgtggtcga tatccagcag
     4681 cgtaccctgc gcaccttcga acatgacgaa atcgccacgc tgacgcgcct ggtcgagcag
     4741 gtcagaaacg tcaaccacca tagaagtcag gatgtcggca acagccatcg tatcatccag
     4801 aactttctgg taatcaaccg cttcagcttt gtagtagtta accaactgga agttgtgata
     4861 ttccatcact tctttcagtt tttcagcgaa ggtttctttg tcgaaaaggt cgccaacacg
     4921 cagaccgcga cgtgccactt tatcttcata agcaggcccg ataccacgac cggtggtgcc
     4981 gatcgctttc gcgccacgcg ctttctcacg cgcgttatcc agagcaacgt gataatcaag
     5041 gatcagcgga catgcttcag acagcagcag acgctcacga acggggatgc cacggtcttc
     5101 cagttctttc atctctttca tcagcgcggc cggagacagc acaacaccgt taccgatgat
     5161 gctggttaca ttctcgcgga gaatacctga tggaataaga tggagaacgg ttttttcacc
     5221 gttgattacg agagtatggc ctgcgttgtg accgccctgg tagcgtacaa catatttagc
     5281 ccgttcagtc agaagatcga cgatcttacc tttaccttcg tcaccccatt gggtgcccag
     5341 tacgacgacg ttgttaccca tttttcaaaa tcaccgtttg cttaaaaatg gattctacca
     5401 tcgctttttc agagttacag cactttttgc actcaaaaat gacctcgatc agtctgtttt
     5461 ttgttcagcc aatcgttttc ctcaacatgt agtagaccac aacgcccgca accacaagtc
     5521 caccgccaaa acgacgtaaa atattatcgg gcaaattggt catcgcagag atcatcttct
     5581 tccatgcctt cgggtaaagc atcggcccta aaccttccag taccaaaacc agggcaagcg
     5641 ccagccagat tgtcgaattc attcattatc cttatagaaa aagaaaacca ccgacatccc
     5701 tgaggatgcg gtggctttat tgacctgtac cgcagtcgtt atattaacgc gttgcggaag
     5761 tcggcgtctt catgtagcgg aagaaatcgc tatccgggct catgaccatc acgtcctgat
     5821 tgccagagaa gctgttctca taagcacgca ggctacggat gaatgcgtag aagtccggat
     5881 ctttactgaa tgcatcagca aacagtttgg ctgcttcggc atcgccttca ccacgcataa
     5941 tgcggccctg acgctcagct tctgccagcg ttctggtcac ttcatagtcg gcagtcgcgc
     6001 gcagtttttc cgcttcttcc tgaccttgtg aacggtgacg acgcgctacc gcttcacgct
     6061 cggcgcgcat acggttgtag atcgcttcag acacttcggt cggcaggttg atctgcttga
     6121 tacgcacatc gacaacttca atacccagcg ccgccatact gttcgggttg atgaccggaa
     6181 ctttgccctt cgtctcagcc gttacgcgct ctgccgcttc ggcaatggcg ttatctgctg
     6241 ccggggtagt aacttcatct tctgtacccg cagaaccgga gttcagcgcg tcacgtactt
     6301 cgagggtcag acgaccacgg gaatcggtga cgatatcttt cacgtccagg cgaccaattt
     6361 cagaacgcag acggtcagag aacttacgtt tcaacagcac ttccgcttgc gaaatgtcgc
     6421 caccacccgt tgccaggtag taacggctga aatcgctgat gcgccatttg atgtaagagt
     6481 cgacgatcag gtctttcttc tctttggtca caaagcggtc ggcctggttg tccatggtct
     6541 gaatacgtgc gtcgagcatt ttcaccgttt caatgaacgg tatcttgaaa tgcagaccag
     6601 gctcataaac caaaggtttg ttgtcatcgt cacgcagtac cttaccaaaa cgcagcgtaa
     6661 taccgcgctc accttctttg acgacaaaga cagacatgta aagcactacc agcacgatga
     6721 tgataatcgc gataactgac ttacgcatcg ttattccccc tgacgctggt agtcgttacg
     6781 ctgcgcgttg gcgcggcgtt ggtccataat atcgccctga ctggtggacg acgtgttgct
     6841 tgctccactg gttgtggagg aagaggctgg cggcagacgc agcagattgc tggcaccgtt
     6901 atcgctcttc gccgcagggg cgttaccacc tttcagcatc tggtctaacg gcagaaccat
     6961 caggttgcca cctttatcgt taaccagcac tttgcgggtg ttacccaaca ctttttccat
     7021 cgtctcgata tacagacgct cgcgagtaat ttccggcgcg gctttatatt ccggcagaag
     7081 tttagcaaag cgcgccactt caccctgagc ttccaggatg gtctgggcct tgtacgcacg
     7141 cgcctcttcg aggatacgtt gcgcctgacc gttcgcacgc ggctgaactt cgttggaata
     7201 cgcttctgct tcacgaatgt attgctgttc gttttcacgc gcggcaatcg catcgtcaaa
     7261 cgccgctttt acttcttccg gcggacgagc agcctggaag ttgacgtcca gcagcgtgat
     7321 acccatgtca tacggacgaa tcgtctcttc cagttcgcgc tgagtatcgc tacgaatcac
     7381 ggtacgacct tccgtcagaa tgcggtccat ggtgtattta ccgataactc cacgcagggc
     7441 gctgtcggta gcctgacgca ggctgtcatc cgggctggtc acgctataca gatatttttc
     7501 cggattggtg acgcggtact gcacgttcat ctcaacgcgc actacgttct cgtccgacgt
     7561 cagcatcaca ccagaagcgg ccagttcacg cacggcttcc acgttcaccg gtttgacttc
     7621 gtcgataaac gtcggtttcc agttcagacc cggctcaacc agatggctga atttaccaaa
     7681 gcgtgttacc acgccgcgtt cggcttcttt aatggtatag aaaccactgg ccgcccagat
     7741 aatgacaatc gctgccgctg cgatggtaac gacacgaccg ccaagctgcg ggcgcgggcc
     7801 ttgcgatgaa ctgccaccgc cagatccggt gcctttaccg ccgcccagac caccgagctt
     7861 tttgctcagt ttgcggaaga tatcatctaa atcaggtggc ccttgatcgc gaccgccttt
     7921 gtttccattt ccctcagagt tgccgccagg tttgctgctt ccccacgggt cgcggtcttg
     7981 tccgttatta ccgggctgat tccacgccat gtttgtgctc catatttgtt atgcggtgat
     8041 ccctgttgtc tttcgacgcc agaggatatg actccacgct tcagacgcta cgccgttaga
     8101 tcaggtaatc gatcaacgcc ggttcttgtt tacagaggcg acgccagtca acgatcggca
     8161 tacgaacttg cagacttacg ctgccgtcct cctccatcca ctctttttct attgcctgaa
     8221 gctgataaaa acgactcctc agacgccctt cctgcggtgg cagacgcaat gtatgctgcg
     8281 ccacctcgcc ggaaagccgc tccgtcaaag cctgaaaaag ctgtggtatc cccgctccgg
     8341 tctgtgcgga aagccagaca cggatcggtt tgttctcttc gtcccgatca atacgcggtt
     8401 cgaaatcttc cagcatatcg atcttgttca tcaccagcag ggttgggatc tcgtgagcgt
     8461 cgatctcttc aagaaccgta ttcaccgctt cgatgttttc ttgtacacgc acatccgccg
     8521 catcaatgac gtgcagcagt aatgtggctt gccgcgtctc ttgtaacgtg gctttaaatg
     8581 ccgccaccag atcgtgcggc aggtggcgaa taaaccctac ggtatctgca agtacggttt
     8641 caccgacatc tgcaacgtca atacgccgca acgtcgggtc gagggtggca aacaactggt
     8701 ctgccgcgta gacccgcgct tcggtgatgc gattgaaaag ggtagattta ccggcgttgg
     8761 tatatcccac cagcgaaaca gtaggaacgt cggctttgat acgcgattgc cgcccctgct
     8821 cacgctgctt ttcaactctt tccaggcgcg actgtatctg cacgatgcga ttacgcaaca
     8881 aacgacggtc ggtttcgagc tgggtttcac ccggaccacg caaacctatc ccgcctttct
     8941 gtctttcaag gtgggtccag ccacgcacca ggcgcgtagc cagatggcgc agctgcgcca
     9001 gctcaacctg caacttaccc tcatgggtac gcgcacgttg ggcgaaaata tctaaaataa
     9061 ggccggtgcg gtcgataaca cgacactcgc acaaacgctc caggttacgc tcttgcgccg
     9121 ggctcagggc atggtcaaaa agaacgaccg aagcacccat cgctttgaca gcttccgcaa
     9181 tttcaactgc tttaccttca cctacaaaat actttgggtg cggcgcttta cggctaccgg
     9241 taatcacctg caatgcttcg acaccggcgg aagagaccag agattcaaac tcctggaggt
     9301 cttccatatc tttgtcttgc gtaaaataga tgtgtaccag taccgcctgc tcaccagcat
     9361 cataacggtc aaacaagcgt ataaccctct aaatagatca gcggggaacg caggatcgct
     9421 ggctccccgt gtaaaaaaac agcccgaaac cttattcggt ttcttcgctg tcctgttgcg
     9481 cggaagtatt ctgcgcgctg ctaccatgat ggtagttact gctggtaccg ccaccggcgt
     9541 tgttactgtg atgagaaacc gggcgagatg ggacaacagt agaaatcgcg tgcttgtaaa
     9601 ccatctggct gaccgtgttt ttcaacagga tcacgaactg atcaaaagac tcgatttgcc
     9661 cttgcagctt aataccattc accaaataaa tagaaactgg aacacgttcc cgacgcagtg
     9721 cgttcaggaa cggatcttgt aaagattgcc ccttagccat tctctctttt ccttatatgc
     9781 ttatttgtac tttgaacctt tcgattctga aaaaattgcg cacgatacgt ctcaattgta
     9841 cacattcagc ctgcgatagc accaacaacc tgtaatactt cgtcacgcgc ctgttctggt
     9901 ttttcactgt caagccagtg aaccccttcc caaccacgca gccaggttat ctgccgcttc
     9961 gccaactgtc tcgtggcgca aacacctcga taaaccattt catcgtatga gatttcgcct
    10021 tcaaggtaag accacatctg gcgataaccc acgcaacgaa tggaaggcaa gtccgtatgc
    10081 aaatctcctc gggcaaaaag cgcccggact tctgcttcaa aacctgaagc caacatctga
    10141 tgaaaacgct gctcaatgcg ttgatggagc agttcacggc tcgccggggc gatggcgaac
    10201 tgatgcacct gatacggtag agcgtctcct gacgtttgcg tcagttccgt taaagtttta
    10261 cccgaaatga aaaaaacttc cagtgcccgg gaaagccttt gtggatcatt tggatgaatc
    10321 cttgctgccg caaccggatc tacctcctga agttgacgat gcaatgactc ccaaccttgc
    10381 tctgccgcct gttgctcaat tctggcccgc acttccgggt ctgccgacgg tagcggcgac
    10441 aacccttcca gcaatgcctt gaaatacaac atcgtaccgc ccactaacag tgggatccgc
    10501 cccgccgcgg tgatatcggc catttccgcc agcgcatcgc ggcgaaaatc agcagccgag
    10561 taagcctgcg acggatcgcg aatatccagc aatcggtgcg gcgcggcgag taactcttca
    10621 gcgttcggct tcgccgtccc gatatccatc cctttgtaaa taaggacaga atcaacgctt
    10681 atcaactcta ctggtaaaat tttacgcagc tcaatggcta acgccgtttt accggaggcc
    10741 gtcggcccca tcaaaaaaat cgccttaggc aggctcgcct tactgatatc actcatcttt
    10801 cagggctttt atcgccggat gtaaatcaac agattgtaac agaccacccg gcggcgtttt
    10861 cacaagttgc ggacataacc gttccacgtc cgccagcagg gttatggcct gtgccattga
    10921 ccactgcgca tgttcgctca tcagatttcg tgcaatccac tgcgcaatat tgccaggttc
    10981 gaatacggac tgcttcgcca ggtagcctat cagttcagga atcaagattt gtaaattttg
    11041 ttggcgtaag ggtaaaggca cggccctgat ggtcacatgc tgtgcatctg actggaaatc
    11101 aatacccaat tccgctaggg cagactgcgc tttttctaat gccgattttt cttcgccaga
    11161 aacttttagc cgcaacggaa tcagcagcgg ctgggcgcaa acgggcgctt cacccggcgt
    11221 caattgtgcc tgacgcagcc aacgttctgc cactggcaag gataaaagtg aaatgttgcc
    11281 atcgcgctcc agcaacgcac agtcggaatg gacgatagtc agtacccgac caaaactctg
    11341 actgttcgcc gcaagtgcag gttcctgcgg ttccggcgct tttggttttt gcatcggcgc
    11401 gggcgtttgc aaaagctggc gatacacttc accttgctgt ttctggtagc ctggctgcgc
    11461 attcggccag ggggcagccg gacgactgcc tgaggcgggc gcaggagagt agcgcggagc
    11521 taccggctca cgagctgccg gttctgcaaa gtgattgcgc cccgccgcca cgcggttttc
    11581 cgggatggca cgcggtgcgg gttgtggttc atcgtccagc ggtagcggcg tttccagttg
    11641 ctgttgtagc acgctcagca cgccctgata gataaaatca tgcaccagac gcgactgatg
    11701 gaaacgcact tcgtgtttgg cggggtgtac gttgacgtcc acctgatgcg ggtcgatctc
    11761 cagatacaac acaaatgccg gttgctgatc ggcccccagt ttgtcttcgc aggcctggcg
    11821 gatcgcatga ttgatcaggc gatcgcgcat catgcgacca ttcacgtagc aatactgaat
    11881 ttccgccagt gcgggcgtag tgtgatttgg atcggccacc cagccgcgca gggtgagatc
    11941 gccgtgttgc cattcaatcg ccagcgcttg ttcgagaaaa gcggtgccgc aaatcgcgcc
    12001 taagcgccgt tctttttgcc cgccttccgg cactgcgcgg tactgacgca caattttacc
    12061 gttatgcgac aggttgatcg tgacgtcgaa acgcgccagc gcaatgcggc ggatgatttc
    12121 atcaatatgg ctaaattcgg ttttctcggt gcgcaggaat ttgcgccgcg ccggggtgtt
    12181 gtagaacaga tccagcacct ccagcgtcgt tcccaccgga tgcgctgccg gtttaaccgt
    12241 cacgtccatg tcgcgtcctt cggcataggc ctgccaggct tcctgctgtt ctgcggtgcg
    12301 tgaagtgagc gtcaggcggg aaaccgaact gatactcgcc agcgcctcac cgcgaaagcc
    12361 caggctgata atggcttcga gatcgtccag agaggcgatt ttactggtgg catgacgagc
    12421 cagcgccagc gccagctcat cttttttgat accgcagccg ttatcacgaa tgcggataag
    12481 tttcgcccca ccgcgttcga tatcaatatc gatacgcgtc gcacctgcat cgaggctgtt
    12541 ttccactagt tctttgacta ccgacgcagg tcgctcgacc acctcacctg cggcaatctg
    12601 gttcgccagt tgtggcggta agacctgaat tggcatcaat cgtccttagt ttggcagcgt
    12661 gcgatctggc gtcgtcaccg tactggcagt ttgtgccgtt gcaccctgcg gcgcagattg
    12721 catcggatgc gcaaggaagt aattgcgcag gcctttgtaa atggcttctg ccagctgttg
    12781 ttggtaatcg tcgctcgcca gcaaacgttc ttcgctgttg ttgctgataa aaccggtttc
    12841 gaccagtact gatgggatat ccggcgagcg cagaacgcca aggctggcgt gttctggtcg
    12901 acgtttatgt atttcgccaa tgcgttgcaa ctgactgatc atactggtcg ctacatcata
    12961 ccctacccgc tgggaatgac cgaactgtaa atccagcacc gcctggctta aataggggtc
    13021 agactgactg ttcgccagca catcacccgc cccacccagc agctccgaca gtttctcgtg
    13081 ctgctccagc cagctggcca tttcactgtt ggcgcgacgg ttagagagca cccatacgga
    13141 agcgccagtc gcactacggt tcggtgcggc atcagcgtga atcgacacga ggaaattggc
    13201 gttttgctta tgtgccacat cactgcgccc catcaccgag ataaagtaat ccccgtcacg
    13261 ggttaaaacg cctttaaaca tcggatcgtc attgagcaaa gtacgcaatt tacgcgcgat
    13321 ggcgatggtg acatttttct cccgcgtacc accggggccg atagcgccag ggtcctgacc
    13381 gccgtgtccg gcatcaatag cgatgataat tttatcgcca gtgttagccg tcgcgcgcgc
    13441 tgccggacgc gttaccgtat tactgctgat aacacccgta gtgcggttac tttccgtttt
    13501 aaacggattg cgcgccggtt cgctgacgcg cggtgcgaca accgcaggcg tttcaacgcg
    13561 tttcgcaacc acgggcggcg gaggaggcgg tggcggcaca tcggcgttaa tcgtaaagac
    13621 gacagtgtaa ttgctgccat tctgccgctt caccgcttcg gttttaccgt tttcggtaag
    13681 atcgaccacc agccgtagcg tttgtgcatc tttaggcgtt ccagagcgaa tcgccttcac
    13741 cagattattg ccgctgaaca acaacggcag tccctgaatc atgcccgttt gtttgatatc
    13801 gagcgccacg gtgcgtttgc tttgatggct aaacgcataa tcaggatcgc caataaaact
    13861 caacgttatc cgcgcctgtt gattaccgtt agaaacctga atatcagaga gcgtcgcggc
    13921 acccaccggc gtgcacagca gcagcagcgt cgctaccaac caatttctga tgcgatacat
    13981 catcccgcca cctttcaaag gttaaccggc taaacgcgcc agcaacaatt cacccgcaga
    14041 ggaaaccgca ctcacgcgcg cctcacggcc ttgtgcctga taatcaatgt gtatttcgac
    14101 atccgggtca ggaagaacac ctgtaccttg ttgtggccac tccaccaggc agatggcatc
    14161 gttggcaaaa taatcgcgga tccccataaa ctccagctcc tcgggatcgg caaggcggta
    14221 caaatcaaag tgatagacca ttaagttgtc gagcgtatag ggttcgacca gcgtataagt
    14281 ggggcttttg acattaccct gatgacccag agcctgtaaa aagccccggc taaaggtggt
    14341 tttacctgcg cctaaatcgc catacagata gattacggtt gcgccatcgc aggctttcgc
    14401 tacccgctcg cccaggtcta atgttgcctg ctcatcaggg agcggaatta ctcgattcat
    14461 catggttttt atcagtcact tccgggttaa caatacgctg tagcgtggaa aagagatcgg
    14521 ttgccagcat cccgcgcgtt ccaaaacgcg ccgccagtac gtcagctgcc gcaccgtgcg
    14581 cgacacagcc tgcacaggct gcatcatacg gcgacagttt ttgcccaagc aatgcgccaa
    14641 taataccaga gagcacatcg cccatgccgc cgctcgccat gcctgcattt ccggcatcaa
    14701 taatgcctaa agcgtcagga tgggcggcga ccacggttcc ggcacctttc agcaccgcta
    14761 cgccgccata acgttgtacc agacgtttgg cgcaatgtaa gcggtcactt tcaatttcag
    14821 cgacggaaca gcctaacaac cgtgcggcct cgctaggatg cggcgtgatc acgcgattgt
    14881 gacgcttatc gggattgatt gccagcaggt tcaatgcatc ggcatcccac aacatcggtt
    14941 tgcgaaaatt ctcaactttt tgcagggctt ttttccccca ctcttgctgg cccagaccgg
    15001 gaccaatcac caccacatcg gcccattcca ggctttcggt aagagagtcc atagtcagtt
    15061 catgcaccat caattctggt cgtgcagtca gcagcggcgc aatgttctca ctgcgggtca
    15121 gtactcggac taaaccagca ccagcacgta gcgccgcttc ccccgtcata cgaatagccc
    15181 ccgccgtgcc gtgatcgcca ccgataatca ccagccgccc gtgatcgcct ttatgcgaag
    15241 tcgggcgacg cggtttcagc cagtgagaaa gttgttctgc cgaaaaccgc tgaattttcg
    15301 tctcctgacc tgccagccag ctatccagcc ccagtgagtc aaaatgcagt tgtcctgtaa
    15361 catcccgcgc ttttccagtg agcaagccta gtttcagcgc aataaaagtg atggtgtgat
    15421 cggcgttgat cactgcgcct ggcgtagcgc cggtttcagc cagcaggccg gaagggatat
    15481 caaccgccac aatcggcgca ggatgggaat tagcgtggtc gattaactgg ctaatggatt
    15541 cgcggggcgc ttgctgcaag ccggtaccga gcagcgcatc aacaatcaga tctaccgatt
    15601 cgggccagac aatattcgaa gcatggatct cgccacccgc gtttaaccat gcttcgcgtg
    15661 ccagcgcggc ctcttccggc aacggtttgt cgctctcctg ggccaacaac gtgacctcaa
    15721 tgccgaccgc tttggccagt cgcgcgacca cgtagccatc gccgccgtta ttaccatgac
    15781 cgcacagcac cagccagtgg cgggcgtcag gatacgccga acgacacacc tggaatgcgg
    15841 cctcgccagc gcgaagcatc agctcataga gtgtgagccc cagcacatct gccgcctcgc
    15901 gttctccgcg gcggatatcg tcggcgtacc agacggtgtg tggtatactt acggggtttt
    15961 tcttcattgt atggtccgtc atgtcagagc ccctcgatct caatcagtta gcgcaaaaaa
    16021 ttaaacagtg ggggctggaa ctgggctttc agcaggtagg tattaccgat accgatctca
    16081 gcgagtccga gcccaaactg caagcatggc tggacaaaca ataccacggc gaaatggact
    16141 ggatggcacg tcacggtatg ctgcgcgctc gcccccatga gttattaccc ggtacgctgc
    16201 gtgtaatcag cgtgcggatg aattaccttc ctgctaacgc cgcatttgcc agcacgctga
    16261 aaaaccccaa actcggctat gttagccgtt atgcgctggg ccgtgactat cacaaacttc
    16321 tgcgcaaccg actcaaaaag ctgggcgaga tgattcagca acattgtgtt tcgctgaatt
    16381 ttagaccgtt tgtcgattct gcgcctattc tcgagcgccc gttagctgaa aaagctgggc
    16441 tcggctggac aggtaagcac tcacttatcc tcaatcgcga ggccggttcg ttcttctttt
    16501 taggcgaatt gctggtcgat attccgctgc ccgtggatca accagtcgag gaaggatgcg
    16561 gcaaatgcgt ggcctgtatg acgatttgcc cgaccggtgc catcgtcgag ccatataccg
    16621 tcgatgctcg ccgctgtatc tcttatctca ccatcgaact tgaaggggcg ctcccggaag
    16681 agttgcgacc gttaatggga aaccgtattt acggttgcga tgactgccag cttatctgcc
    16741 cgtggaatcg ctattcgcaa ctcactacag aagacgattt cagcccgcgt aagccgctac
    16801 acgcaccgga actcattgag ttatttgcct ggagcgaaga gaagttttta aaagtcacgg
    16861 aaggatcggc gattcgccgt ataggtcacc tgcgctggct gcgtaatatc gccgtggcat
    16921 taggcaatgc cccttgggat gaaacgattt tgacagcgct ggaaagtcgt aaaggtgagc
    16981 acccacttct cgatgagcac atagcgtggg cgattgcgca gcaaatagag agacgaaatg
    17041 cgtgcatagt cgaagtgcaa ttgccgaaaa aacagcgtct ggttcgggtg attgaaaaag
    17101 ggttaccgcg tgacgcctga ttcatccaca gcttgtgaat aaaaataaaa acgcattgca
    17161 attcaagacg aataaaatcg tcaagtgatc gactcgacaa aacggaatct tgatttttaa
    17221 cttatttatc aataggttaa aaaaatacca ttcaccgtgc gaagtttctt tgaaattaag
    17281 attacagcct gaggctgtgg ataactctgt ttacaagagt ttgtcagaga caacaaaaac
    17341 cagcaataac gcatcgcgtc gctgtagata ttttattgag agaagaattt ggagcgggaa
    17401 acg
//
LOCUS       DAZPCS010000078        17349 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZPCS010000078 DAZPCS010000000
VERSION     DAZPCS010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17349
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.078"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            186..788
                     /gene="ais"
                     /locus_tag="ACHUQ7_002444"
     CDS             186..788
                     /gene="ais"
                     /locus_tag="ACHUQ7_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFR1135814.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADISDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDAIFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(827..1252)
                     /gene="nudI"
                     /locus_tag="ACHUQ7_002445"
     CDS             complement(827..1252)
                     /gene="nudI"
                     /locus_tag="ACHUQ7_002445"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFR1135815.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            1531..2073
                     /locus_tag="ACHUQ7_002446"
     CDS             1531..2073
                     /locus_tag="ACHUQ7_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFR1135816.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGLYVGVNASF"
     gene            2160..3362
                     /locus_tag="ACHUQ7_002447"
     CDS             2160..3362
                     /locus_tag="ACHUQ7_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFR1135817.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVIPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            3426..3887
                     /locus_tag="ACHUQ7_002448"
                     /pseudo
     CDS             3426..3887
                     /locus_tag="ACHUQ7_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
     gene            complement(3928..4878)
                     /locus_tag="ACHUQ7_002449"
     CDS             complement(3928..4878)
                     /locus_tag="ACHUQ7_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFR1135818.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQSHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLVGLQQGKLEGLQ
                     EGMHEQAIKIALRMLEQGIDRDQVLAATQLSEADLAANNH"
     gene            complement(5071..6261)
                     /gene="glpC"
                     /locus_tag="ACHUQ7_002450"
     CDS             complement(5071..6261)
                     /gene="glpC"
                     /locus_tag="ACHUQ7_002450"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFR1135819.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(6258..7517)
                     /gene="glpB"
                     /locus_tag="ACHUQ7_002451"
     CDS             complement(6258..7517)
                     /gene="glpB"
                     /locus_tag="ACHUQ7_002451"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFR1135820.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(7507..9135)
                     /gene="glpA"
                     /locus_tag="ACHUQ7_002452"
     CDS             complement(7507..9135)
                     /gene="glpA"
                     /locus_tag="ACHUQ7_002452"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFR1135821.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            9408..10766
                     /gene="glpT"
                     /locus_tag="ACHUQ7_002453"
     CDS             9408..10766
                     /gene="glpT"
                     /locus_tag="ACHUQ7_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFR1135822.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            10771..11847
                     /gene="glpQ"
                     /locus_tag="ACHUQ7_002454"
     CDS             10771..11847
                     /gene="glpQ"
                     /locus_tag="ACHUQ7_002454"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFR1135823.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(11889..12131)
                     /locus_tag="ACHUQ7_002455"
                     /pseudo
     CDS             complement(11889..12131)
                     /locus_tag="ACHUQ7_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            12126..12230
                     /locus_tag="ACHUQ7_002456"
                     /pseudo
     CDS             12126..12230
                     /locus_tag="ACHUQ7_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12310..12960
                     /gene="inaA"
                     /locus_tag="ACHUQ7_002457"
     CDS             12310..12960
                     /gene="inaA"
                     /locus_tag="ACHUQ7_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HFR1135824.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(13014..13268)
                     /gene="yfaE"
                     /locus_tag="ACHUQ7_002458"
     CDS             complement(13014..13268)
                     /gene="yfaE"
                     /locus_tag="ACHUQ7_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFR1135825.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(13268..14398)
                     /gene="nrdB"
                     /locus_tag="ACHUQ7_002459"
     CDS             complement(13268..14398)
                     /gene="nrdB"
                     /locus_tag="ACHUQ7_002459"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFR1135826.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            complement(14632..16917)
                     /gene="nrdA"
                     /locus_tag="ACHUQ7_002460"
     CDS             complement(14632..16917)
                     /gene="nrdA"
                     /locus_tag="ACHUQ7_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFR1135827.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPNIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            17045..17188
                     /gene="ypaB"
                     /locus_tag="ACHUQ7_002461"
     CDS             17045..17188
                     /gene="ypaB"
                     /locus_tag="ACHUQ7_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFR1135828.1"
                     /translation="MTLLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     AIS"
BASE COUNT         4091 a         4226 c         4636 g         4396 t
ORIGIN      
        1 tattagacgt aaatattgaa atttttatat tttttcttat ttaggctttg catttggcaa
       61 aaatttgagg cattttgccg acatcgtagg atttttaata ttacaccaac tgctaattat
      121 cgccagaaat gtcgctcaat ttcacggtaa ttgtctggtt gcgcttgtct ataggtggag
      181 tttacgtgtt agctttttgc cgctcatcat tgaagtcaaa aaaatatttc atcattttac
      241 tggcgctcgc tgcaattgcc ggactgggta ctcatgccgc ctggagtagc aatggtttgc
      301 cacgtatcga caataaaaca ttggccagac tggcacagca gcacccggtt gtcgttttgt
      361 ttcgccatgc tgaacgttgc gatcgttcaa ccaatcaatg cttgtcagat aaaacaggta
      421 ttacggttaa aggtacccag gatgcccgtg aactgggcaa cgcttttagc gctgacatct
      481 ctgatttcga tctttattcc agtaataccg tccggaccat tcagtcggcg acctggtttt
      541 ccgcgggtaa aaagctgacg gtagataaac gacttcttca gtgcggtaat gagatttata
      601 gtgcaattaa ggacttacaa agcaaagcgc ctgataaaaa tatcgttatt ttcacccata
      661 atcattgcct gacatatatt gctaaaaata agcgtgatgc gatatttaaa cctgattatc
      721 tggatggttt agtcatgcat gtggaaaaag gcaaagttta tctggatggg gaattcgtta
      781 accactaaat tttcaatctg acagccagtg atggctgtca tcattgttaa agaagacctt
      841 tcaaacgtaa cgtttttcgg gtggcaacat ttaaatcata atgcaccaga tcttcaggtt
      901 ttacccacgc gtagtcctga aactcttcgt ttattttcac ttctcggttg gcagaaacgc
      961 agtcaaaaat caggtaaatc atataaatct cttccttgcg accatctgca tatgtcttgg
     1021 tgcgaatatc atcgctgaag gtccacggcg tgatttctgt caaaagcagc tgttctccca
     1081 gttcttcgcg aatttcgcgg cgtagtgcct cttcaattcg ttcgccagac tccacgccgc
     1141 cacccgaaag cgcccattga ccggggaaaa cgccgcgatc gtcggccatt ttgcacagca
     1201 aataagcacc atcattttga atcaaagggc atacaatagt ccgttgtcgc acaggggtat
     1261 tccttataaa taaaaagaaa ccatgagaat acggtaactc agaaatatgt gaaagtgaca
     1321 gtaatctctt ttgcaatgat aactgcactt attattattt ttgtgtgtat cggtaaagtg
     1381 tgtttatttt ttcggcgttt aattatttaa gaagtattta gttacaataa gtgactagat
     1441 cccttttttc gctcattgaa tcctctgtgc caggcagtac aatcccgcag ctttgggaat
     1501 tttgattaat tgcttgtggg aaaggggata atgaaaaaaa ttgcgctagc aggtctggcc
     1561 ggaatgctgt tggtttctgc atcggtcaat gcaatgagca tcagcggcca ggcgggtaaa
     1621 gaatacacca atattggtgt cggttttggg actgaatcga cgggcctggc tttaagcggt
     1681 aactggacac ataacgacga cgacggtgac gtcgcgggcg tggggctggg gttgaatctg
     1741 cctctcgggc cgttaatggc gaccgttggc ggaaaaggcg tgtacaccaa cccgaattac
     1801 ggcgatgaag gttatgccgc agcggtagga ggtggtttgc agtggaaaat tggcaacagc
     1861 ttccgtttgt ttggcgagta ttactactct ccggattcgc tctccagcgg tattcaaagt
     1921 tatgaggaag cgaatgctgg cgcgcgttac accattatgc gtccagtcag tattgaggcg
     1981 ggttatcgct acctgaatct gtcgggtaaa gacggtaacc gcgacaacgc tgtggctgac
     2041 ggcctgtatg ttggggttaa cgccagtttc tgatcccttc cggcgcggca ttcagtttct
     2101 tactacgcct tatctccctt tgcgttatag tgattcgata acaaaagcag gagataacca
     2161 tgttaaaagt ggaaatgcta tccaccgggg atgaagtgtt atacgggcaa atcgttgaca
     2221 ctaacgctgc ctggctggcc gattttttct ttcatcaggg gctgccatta tctcgccgca
     2281 atacggtggg ggataacctt gatgacttag tcaccattct tcgcgaacgt agtcagcacg
     2341 ccgatgtgct gatcgttaac ggcgggctgg gaccgaccag cgatgattta agcgcactcg
     2401 ccgctgcgac agcaaaaggt gaaggcctgg tgctgcatga agcctggctc aaagagatgg
     2461 aacgctattt ccacgaacgt ggacgagtaa tggcaccgag caaccgtaaa caagcggagc
     2521 tgcctgccag tgctgaattt atcaataacc cggtaggcac cgcctgtggt tttgccgtgc
     2581 agcttaatcg ttgcctgatg ttctttactc ccggcgtacc gtcagaattt aaggtgatgg
     2641 tcgagcacga aatcctgccg cgcctgcgcg agcgtttttc tttaccacag ccgccggttt
     2701 gtctgcgttt aactaccttt ggtcgttcgg aaagcgatct ggcacaaagc ctggacactc
     2761 tacaactgcc gccgggcgta acaatgggct atcgctcctc aatgccgatc atcgaactga
     2821 aactcaccgg accggcaagc gagcaacagg cgatggaaaa actgtggctg gacgttaaac
     2881 gtgttgccgg acagagcgtg attttcgaag gcaccgaagg actgcccgcg cagatcagtc
     2941 gcgaattgca aaaccgccag ttcagcctga cgttgagcga gcaattcacc ggtggtttat
     3001 tggctttgca actttctcgc gcaggtgctc cattgctggc gtgtgaagtg attccttcac
     3061 aggaagaaac cctggcgcaa actgcgcact ggattacaga acggcgggcc aaccattttg
     3121 ccgggctggc actggctgtt tcgggtttcg agaacgagca tctcaacttt gcgctagcca
     3181 cgccagacgg cactttcgct ctgcgtgtgc gtttcagcac tacacgctac agcctggcta
     3241 tccgtcagga agtgtgcgca atgatggcac tgaatatgct acgccgttgg ttaaacggcc
     3301 aggacatcgc tagtgagcat ggctggattg aggttgttga gtccatgact ttatccgtct
     3361 gatttcgcca ctctgtgccg gtttactgca ccggcacatt ccccctccgg caaaatttgt
     3421 ccaaccctcc ctacggtgag cgtggtgttg gggccagtgt ggcacgggct gcgcgctggg
     3481 gacgcattga gaattacatg gcgcaagtca acgattcgct ttgtctgtta gtgcaggtgg
     3541 aaagtaaaac ggcactggat aacctggacg agatcctcga cgtcgaaggg attgatggcg
     3601 tgtttattgg accagcggat ctttctgcgt cgttgggcta cccggataac gccgggcacc
     3661 cggaagtgca gcgaattatt gaaaccagca ttcggcggat ccgtgctgcg ggtaaagcgg
     3721 ctggttttct ggctgtggct cctgatatgg cgcagcaatg cctggcgtgg ggagcgaact
     3781 ttgtcgctgt tggcgttgac acgatgctct acagcgatgc cctggatcaa cgactggcga
     3841 tgtttaaatc aggcaaaaat gggccacgca taaaaggtag ttattgatat caaaggccca
     3901 tggggatcgg ctgtgggcct gtgttaatta gtggttattc gctgccagat cggcttcgct
     3961 tagctgggtg gccgcgagca cttggtcacg atcaatgccc tgttccagca tgcgcaaagc
     4021 aattttaatg gcttgttcat gcataccttc ctgcaaacct tctaatttac cttgctgcaa
     4081 acctactaat ttaccttctt gccagccaat ttgatgtcct tcctgccgta gccgttcagc
     4141 aatagtcatt aatctctcct tttgtagtgg tgaacgttcg gcaatctcct gaataaaacg
     4201 ggtgaaacgg gaggtatcgc cgcattgcag cagataatta aacagtgttt gtagctggct
     4261 gtcattagtg aagcctctaa ccaaaagcgt ggtgatcctg tcgaccatgc cgattaaatc
     4321 gtggtcgcga atatgctttt gaatcagttc cagcagagct atacgccgat gttgcatgat
     4381 ctcatcgtca ggtacgatgg tgatatccac caacggaaaa gcttcggtgt acaactgccg
     4441 ggcaagttgc ggatcgtcaa actcatccag ccagttaagt gagtacgggt agggcgatgt
     4501 ttcgccatga taaaacagca atggcaccac cagcggaact ctgtcatagc ctttatctag
     4561 gtgactctgc atggcggcag tggcatagcg cattggccga aaagccatat tcttttctgc
     4621 agagctttga tgttcaatca cgcagtagat atagccgtca ccgtcgctgg tttccacgga
     4681 ccacaaaaca tccgagtagt aagcgcgtaa acttttttca ataaaactag tgggttccag
     4741 gcgtaaggtt tgcaggttgc aaagcttgcg cagtggttct ggtaaatgta tttcgagaaa
     4801 atcccgtgcg gtttcgggtg tgaatataaa ggttttaaat accgcatcat gcggggagga
     4861 ggttgttgat tcggtcatgg ttgtccgtca cctgatattt atgatgacgc gacattacgg
     4921 ttatcccagg cctttgacat ctgccagttt cagcctttgc gagacgtttt cagaggaatt
     4981 ttcactgcgt tgcaacgcat acattgggca cggaatcgtg tacgcaaaaa tgcccggtaa
     5041 ccacatgctt accgggcatc agaagggagt ttaagccagc gcctgggcca gtagcgtaat
     5101 cggatgttcg cagcgaagac ttgtggacat ctcaatctgc catttacagg tttcgcagtc
     5161 ggtgaccacc agatctgcgc cgctttcttc tatctggcgg aacagtggtg cgccgatggc
     5221 ttgtgaggtg gggtagttct cttttttgaa accgtaagta cccgcaatac cgcagcactg
     5281 ggaatccagc accgttaact caagccccgg gatgttacgc aacagctcca gggtgtagag
     5341 cgtccagccc attttttcca tatggcacgg agtgtgataa accactttca gcggcagcgg
     5401 tttcagcggt aacgttttgc cttcgtccag cttgcgccac agccagcggg ttgccagttc
     5461 gatatgatcg cgcaagcctt tgttgtcgac attcagcact tccgggtatt cgtcgcgcag
     5521 ggcaaatgta caggttgagg aggtggcaat caccggaatg ccttttactc ccacagcttc
     5581 gcggatcgac tctacattcg taattgcctg tttgcgtgct ttatcggtaa agccgttggc
     5641 gattagcggt acgccgcagc atttttcttt gctgagcagt tgtacaccgg tacccattgc
     5701 gttgagcact ttaattaaat ctttacctaa ctgcggatgg ttgtagttaa cgaagcagcc
     5761 gtgaaagaaa gcgacctggt ctttatattg tgcttgctga gccgctatgc tgcgatacca
     5821 gcgacggaac gtgccgaagg agtatttcgg tagcgtgcga cgatgatcga tttttaacgc
     5881 cgcatcaagc agctgccgca ccggtttcag cgaggtagcg gtgttgacga ttggtgcgaa
     5941 cggcgtggaa acgctaccca tcaggtcggt atgactcaac acaaaattac gcagcgacgg
     6001 gcgcgtggtg tcatatttcg cccgcgcgcg ctggataata tcgccaatct tcacatcgga
     6061 cgggcaggcg acttcacaac gtttgcagtt gatgcaatat ttcagcgcct cgtcatacag
     6121 tgcgccatct ttcaaacgca gacgctcgcc atccggcccg gcttgttttg gccctggata
     6181 accgggattc acccggctca ccgggcaggc ggtggtgcag acggtgcact taatgcagtt
     6241 ttcgaagctg gtgtcgttca ttgttggcct cctgcgcgtt gggcaatctg ttgagcggca
     6301 tgtaaagcac tgacggcaca aacaccgccg ccgcatccct gggcgatggg atcaaatccg
     6361 cccagcaccg aaccgatggc aaacaggttc tcaatggttt gccctgcctg cgacgggcgt
     6421 agcgtctcat cagtggttac accgaactgc tgccacggtt gcggcgcaaa aaaatcgccc
     6481 ttataccatt caccccgcgt ggcggtttgt agcacatcaa ggccgagaat cggctcgcga
     6541 atgccgttac gttccgctac tagtccgcca ctaaagaaac tgccgctggc gagaaccgcg
     6601 aaacgtggac gtagcggaat atcggcgtga ttgcgggtcc agatttcatt cactacgcca
     6661 tttttacagg tcactttttt cacttcatcg cccggcatcc acactccacc ttggcgcaca
     6721 aactggcgct gtaactggtt ttgcagacga atgcccagca cggaaggcgg cagcgttggc
     6781 aaaagcatca gtgaacaagg tagtttttca ttcaaccaac gccacagttt gtcatcggcc
     6841 aggccgaagc aggcgggcat caggatcatt tcgcaggtat tggcgacagg aataagcgca
     6901 tcaagtaaca gcggccagtt ttcttcatta tcaaggaaac gggcgatatt caccgcgcga
     6961 aattcggtgg cgttattgcg caggacatcc agttccggca gctctatttc tgcggtttca
     7021 accgaaagat ccagttcgcg caacgaagcc gccgcaagat gcgcctggaa atccatcagc
     7081 ccgcttatcc ccactacaca tatcttcttc gcgggtagcg gccagacggg aacttctggc
     7141 gaacttaacc aggttgcacg caaagtgccg agcggcgtaa tccgctggtg agccagttct
     7201 acgctgccct gcaattgcgc accgctttca gcgattaatg cctgcgcctg gcaagcgaga
     7261 tcgagcacgc gttgtggccc gagaagagag taaggatgtg caggtgcctg ctgacgcaaa
     7321 gattccagtc cactgtgaat gtctgccacg ggttgaccat ctggcagatg gctcagcaaa
     7381 tccagcgaac cggatgagaa atgcaacgcg ctttgaccac gcgtgacaat ggcacagcgc
     7441 aggccgtgtt tttgcagttg caggccacag agtaatccgg cgaggccgcc gcccataatg
     7501 acagtatcaa agcgcatctt tctgctcctt ctccagacca cacagtccct gataaaccca
     7561 gcgggtaaat tcgctttcgc gcagtgcgtc tccccaggcg atgggttgca cgcctttcca
     7621 gcgttcgtta aggaaggtgg aaagttgctc gatagattgc gcggacgtcg tgacgttaaa
     7681 ccgttgcagc agtccggcag cgcggcaggc gcagagttcg ccctggcagg tgcccatccc
     7741 cacacgggta cgacggcgta aatccagcag gctattaacg tttaaatttt ctaccgcgta
     7801 ctgcacttca cccgcagtta ccgcttcgca ctcacatacc aggctacggt gtagacggcc
     7861 ttcgctcagc caggcaggcg tgcgatcgcc atgacgataa accgcagaac cgcgcagcgg
     7921 ggcaggcagg gagatgactt tacgcaaggt aacttcagcg ggttcttgtg aaccaggcag
     7981 tgccagatcg gcagtcgtac aggggcgcgt gttgcccagt ttgcggcata ccgcgtcggt
     8041 agcccattca gccatcagcc gataggtcat cagtttgcca ccggtgatgg tgataaatcc
     8101 gtccagacca tcgcgttcag catggtcgag cagcacgatg ccacggctga cgttacgtcc
     8161 gctcgggtcg tcatcgctgg caaccagcgg gcgcacgcca gaataggccc gcaaaatgcg
     8221 cgttttcgcc atcacggggg ccagtttttc cccttcacgc agcagaatat caacctcttc
     8281 tgccgtcact cgattatcgt caatctcgtt gtaatcaata cgtaaagagg tggtaccaat
     8341 cagcgaaatg gtatcgccag gcaccagaat atcggcgtcg gaaggtttac ggcagcggtt
     8401 gatcacatgc tggttaatgc ggtgatccat gatcagcagc gatcctttcg ccgggaacat
     8461 gcgaatgcgc agatcggcat attcggcaat gtgttgcccc cagatcccag cggcattaac
     8521 cacgacaggt gcatgaaggg cctgagtttc gccggtgaga tggttacgta cacgaacacc
     8581 gcacaccgtc gcgccttcac gaatcagccc cgtgacttca tgagcggtaa ggataacggc
     8641 accgtgttct ttggcatcca gcatgtttgc tgcggtcaga cgaaatggat caacggtgcc
     8701 atccggaact ttcaccgcgc caatcagtgc cgggttaacg gcaggttcga taatgcgcgc
     8761 ttgctgcggg tctatagctt ctgcgctgat ccctgcttct tcgcaggcgc gaataaaagt
     8821 ggcctggaat gagaggtcat cttccggcag ggtgataaac aggccgttgg ttggttcaac
     8881 gcagtgacgt gcaatgcgtt tcaggatctg gttttcacta atgcattcgc gggccgattc
     8941 cgcatcggtt actgcatagc gcgcaccgct gtgcagcagg ccgtggttac gcccggtggc
     9001 accggttgcg atgtcgtggc gctcaaccaa aatcacgcgc agcccgcgca gggcacagtc
     9061 gcgggcaatc ccggctcccg ttgcgccgcc gccaatgata atcacgtcac ttgattgcga
     9121 gtcgcgagtt ttcattgtta tccctctgaa gttcgttttt taccatttag ccatagtaaa
     9181 aacatgaatt gtttgatttc gcgcatattc gctcatgatt cgaaagtaaa acgtgatttc
     9241 atgcgtcatt ttgaacattt tgtaaatctt atttaataat gtgtgcggca attcacattt
     9301 aatttatgaa tgttttctta acatcgcggc aactcaagaa acggcaggtt ctctcactga
     9361 atcaggctgt taatcataaa taagaccacg ggccacggag gctatcaatg ttgagtattt
     9421 ttaaaccagc gccacacaaa gcgcgcttac ctgccgcgga gatcgatccg acttatcgtc
     9481 gattgcgctg gcaaattttc ctggggatat tctttggcta tgcggcttac tatttggttc
     9541 gtaagaactt tgcgcttgct atgccttatc tggttgagca gggattctca cgcggtgatt
     9601 taggttttgc cctttcgggg atctcgattg cttatggatt ttcgaaattc atcatgggtt
     9661 cggtatcgga tcgctcgaat ccgcgcgttt tcctgcccgc aggtttgatt ctggcggcgg
     9721 cagtgatgtt gtttatgggc tttgtgccat gggcgacgtc gagcattgcg gtgatgtttg
     9781 tactgttgtt cctctgcggt tggttccagg ggatggggtg gccgccgtgt ggtcgtacta
     9841 tggtgcactg gtggtcgcag aaagaacgtg gcggcattgt gtcagtgtgg aactgtgcgc
     9901 acaacgtcgg tggtggtatt ccgccgctgc tgttcctgct ggggatggcc tggttcaatg
     9961 actggcatgc ggcgctctat atgcctgctt tctgcgccat tctggtggca ttattcgcct
    10021 ttgcgatgat gcgcgatacc ccgcaatcct gtggcttgcc gccgatcgaa gagtacaaaa
    10081 atgattatcc ggacgactat aacgaaaaag cggaacagga gctgaccgcg aagcaaatct
    10141 tcatgcagta cgtgctgccg aacaaactgc tgtggtacat cgccatcgcc aacgtgttcg
    10201 tttatctgct gcgttacggc atcctcgact ggtcaccgac ttatctgaaa gaggttaagc
    10261 atttcgcgct ggataaatcc tcctgggcct acttcttcta tgagtatgcg ggtattccgg
    10321 gcaccctgct gtgcggctgg atgtcggaca aagtcttccg tggtaaccgt ggggcaaccg
    10381 gcgtcttctt tatgacactg gtgaccatcg cgactatcgt ttactggatg aacccggcag
    10441 gtaacccaac cgtcgatatg atttgtatga ttgttatcgg cttcctgatc tacggtcctg
    10501 tgatgctgat cggtctgcat gcgctggaac tggcaccgaa aaaagcggca ggtacggcag
    10561 cgggctttac cgggctgttt ggttacctgg gcggttcggt ggcggcgagc gcgattgttg
    10621 gctacaccgt ggacttcttc ggctgggatg gcggctttat ggtaatgatt ggcggcagca
    10681 ttctggcggt tatcttgttg attgttgtga tgattggcga aaaacgtcgc catgaacaat
    10741 tactgcaaaa acgcaacgga ggctaatggc atgaaattga agctgaaaaa ccttagcatg
    10801 gcgatcatga tgagcactat agtcatggga agcagtgcaa tggcggcgga cagcaacgaa
    10861 aaaatagtca tcgcccatcg cggtgccagt ggatatttgc cggagcatac gctgccagca
    10921 aaagcgatgg cgtatgcgca gggagcggat tatctggaac aggatttggt gatgaccaaa
    10981 gacgaccatc tggttgttct gcatgaccat tatctcgatc gtgttactga tgttgccgat
    11041 cgtttcccgg atcgggcgcg caaagacggt cgttactacg cgatagattt cacgctggat
    11101 gaaattaagt cgctgaaatt taccgaaggt ttcgatattg aaaacggtaa aaaagtacag
    11161 acttatccgg ggcgtttccc aatgggtaag tccgacttcc gggtgcacac ctttgaagaa
    11221 gagattgaat ttgttcaggg gttaaatcac tctaccggga aaaatatcgg tatctatcca
    11281 gaaatcaaag cgccgtggtt ccatcatcag gaagggaagg atattgcggc aaaaacgctg
    11341 gaagtgctga agaaatatgg ttacaccggt aaagacgata aagtttattt gcaatgtttt
    11401 gatgctgatg agctgaagcg tattaagaat gagctggaac ccaaaatggg catggagctc
    11461 aatctggtac agctgattgc ctataccgac tggaatgaaa cgcagcagaa acagccggat
    11521 ggaagctggg ttaattacaa ctacgactgg atgtttaagc cgggagccat gaaacaggtg
    11581 gcggaatatg cagatggtat tggtccggat taccatatgt tgattgagga gacatcgcag
    11641 ccgggtaata tcaaactcac tggcatggtg caagatgccc agcagaataa actggtagtg
    11701 catccttata ccgtgcggtc agataaactg cctgaataca ctcctgatgt gaatcagtta
    11761 tatgatgctc tgtataacaa agcgggtgta aatgggctgt ttactgattt ccctgataag
    11821 gcagtaaaat ttcttaataa agagtaatgt tactgcagga tggcgttcat gcgccatcct
    11881 gtctcatatc aaatttttcc aatcgccact aattgttcca tgcacatttg tagtaaaaac
    11941 agtggactgc cttctaccgg cttttcttta gtcagcaacg aaatctgtcg atagaaggtt
    12001 ggttcgtgag gaacggaaca caattcacct gcaatgcttt tcagcgttaa ctctggttgt
    12061 aaggcgatgc ctaatccctg tcgaataaaa ttcatcgctg tatccgactg attaaattca
    12121 cattttatct gctgggcgca ctattagtgg tagtggctgg tggaatctat gcgctgcaaa
    12181 tgaagggcaa aagcggtgtc acacctgtag cggcaaaaga aattcattaa tacgacacgt
    12241 ttcattaaga ttttcctcag gtaaccacct atagtcattg tcgttaataa aaatgacagg
    12301 cgatagggta tggcagtttc agcaaagtac gacgaattta atcactggtg ggcaacagag
    12361 ggcgactggg ttgaagagcc taactatcgc cgtaatggta tgagcggggt gcaatgcgtc
    12421 gagcgcaacg gcaaaaagct gtatgtaaag cgcatgacgc atcatctgtt tcattcagta
    12481 cgttatccgt tcggccgacc aacgattgtc cgtgaggttg cggtaattaa agaactggaa
    12541 cgggccggtg tcatcgtgcc gaagatcgtt tttggtgaag cggtgaaaat tgagggtgaa
    12601 tggcgcgcgt tgctggtgac tgaagatatg gcggggttca tcagcattgc tgactggtat
    12661 gcccggcatg cagtatcgcc ttattctgac gaagtacggc aagccatgtt gaaagcagtg
    12721 gcactggcgt ttaagaaaat gcatagcgtt aatcgtcagc atggctgttg ctatgttcgc
    12781 catatctatg tgaaaacaga aggtaaggca gaagctggtt ttcttgatct ggagaaaagt
    12841 cgtcgccgct tgcgtcgcga taaagcgatt aatcatgact tccgccagct ggaaaaatat
    12901 ctggagccga tccctaaagc ggactgggag caggtgaaag cgtattacta cgccatgtaa
    12961 tcgttgtagg ccagataata cgcgtaagcg tcgtatctgg cgctagtacc cgatcacaac
    13021 tcgatttcaa tatcgccttt tgcccggcaa caacagggca aaatttcccc cggctgaata
    13081 aaggctaacg gttcggcaat ccagtccacc tgacctgcga ccagtcgggt gcgacaggag
    13141 ccgcagtaac cttcgcggca ctggtactca accgccacat tgtgggattc cagcgccgcc
    13201 agaagggaag ggtgttcatc ctggcacagc agttgtgtgc cagtgatgcg cagggtaacg
    13261 cgggccatca gagctggaag ttactcaaat cgtcggtgtc cacttccgcg tcaatctgcc
    13321 cgaccagata agaactgact tccacttcct gcggagcaac ctgcacgtta tcagacacca
    13381 gccaggtgtt gatccacggg atcgggttgg agcgcgtctg gaacggcaga tccaaaccga
    13441 ctgcctgcat gcggatattg gtgatgtatt caacgtactg gcagagaata tctttattca
    13501 gaccaatcat cgaaccgtca cggaacagat aatccgccca gtctttctct tgctgtgccg
    13561 cctggacaaa caggtcatag cactcctgct tacattcttc ggcaatttcc gccatctccg
    13621 gatcatccgc gccgctgcgc agcagattca gcatatgctg agtaccggtc aggtgcaggg
    13681 cttcatcgcg ggcaatcagg cgaataatct tggcgttacc ttccatcagt tcgcgttctg
    13741 caaaggcaaa ggaacaagca aagctgacgt agaagcggat cgcttccagc gcgttaacgc
    13801 tcatcaggca gagatacagt tttttcttca gctcgcgcag gctaacggtt acggttttac
    13861 cgttaacggt gtgggtgcct tcgcccagaa ggtgccagta gctggtcatt tcgatcagtt
    13921 catcgtaata gctggagatc ccttccgcac gtttctggat ctgctcgttg gtgacgatat
    13981 cgtcaaacac aacagacgga tcgttaacga tattacgaat gatatgagta taggaacggg
    14041 aatgaatcgt ttctgagaac gcccaggttt cgacccaggt ttccagttcc ggaatagaaa
    14101 taagcggcaa tagcgccacg ttcgggctac gaccctgaat ggaatccagc agcgtctgat
    14161 atttcaggtt gctgataaag atgtgttttt cgtgctccgg cagcgcctgg taatctatac
    14221 ggtcgcggga gacgtcaact tcttccggac gccagaagaa agagagctgc ttttcgatca
    14281 gcttttcgaa gatgtcatat ttttgctgat cgtagcgagc cacgttgacc ggctgaccaa
    14341 agaacatcgg ttctttgagc tgatcatttt tcgtctgtga aaaggtggta tatgccatga
    14401 gtgtgtcctg ttgggagttt aatgccggat aaggcatttt acgccgcatc ctgtgcctga
    14461 tgcgacgctg gcgcgtctta tcaggcctac aaatccgagc cgtaggccgg ataaggcgtt
    14521 cacgctgcat ccggcacccg gagcctgatg cgacgctggc gcgtcttatc aggcctacaa
    14581 accgagccgt aggccggata aggcgtttac gccgcatccg gcatctcaat atcagatctt
    14641 acatgcgccg ctttcgcagc catcgtcctg gattgacggt accagatcgt cctgcgcgtc
    14701 ttcagcgccg tcacgggtgt tctgataata cagcgttttg accccgaatt tgtaggcggt
    14761 gagcaggtct ttcagcaact gctgcatcgg cacttttcct gacgggaaac gtgacggatc
    14821 gtagttggtg ttagcagaga tcgactgatc gataaatttc tgcatgatac ctaccagttg
    14881 caggtagcca tcgttacccg gcatttccca cagcagctca taggcgtcgt gcaggtgctc
    14941 gtagtccggc accacctggc gcaaaatacc gtctttcgac gctttgatgc tgacgtaacc
    15001 gcgcggcggt tcaataccgt tagtggcgtt agagatctgc gaagaggtct cggacggcat
    15061 cagggcagaa agcgtggagt tacgcaggcc gtgcgttttg attgattcac gcaatgcttc
    15121 ccagtcgtaa tgcagcggct cattagcgat ggcatccaga tctttcttat aggtatcgat
    15181 cggcaggatc cctttcgcgt aagtggtttc gttaaaccac gggcacgcgc cttgctcttt
    15241 agccagctca ttagacgctt tcagcaggta atactgaatc gcttcgaagg ttttatgcgt
    15301 caggttgttg gcgctgccgt cggagtagcg tttaccgtgc ttcgccagat agtaagcgaa
    15361 gttaatcaca ccaataccca gcgtacgacg ccccatcgct ccacgtttgg cggccgggat
    15421 cgggtaatcc tgataatcca gtagcgcgtc aagtgcacga accgccagaa tcgccagctc
    15481 ttccagttca tccaggctat taattgcgcc caggttgaaa gcagacagcg tacacagcgc
    15541 gatttcaccg ttctcgtcgt tgacgtcgtt cagcggtttg gtcggcaggg caatttctag
    15601 gcacaggtta gactggcgaa ctggcgcgat gttcggatca aacggactat gggtattaca
    15661 gtggtcaacg ttctgaatat agatacgacc ggtagacgca cgttcctgca tcatcagcga
    15721 gaacagctca acggctttca cacgctgctt gcggatgctg tcgtctttct catatttggt
    15781 atacaggcgt tcaaactctt cctgatcggc gaagaacgcg tcgtacagcc ccggtacgtc
    15841 ggacgggctg aacagggtga tatcttcacc tttcagcagg cgggtataca tcagtttgtt
    15901 gatttgtacc ccgtagtcca tatgacgcac gcggttgcct tcaaccccac ggttgttttt
    15961 caacaccagc aggctttcca cttccagatg ccacatcggg tagaacagcg ttgccgcacc
    16021 gccgcgcaca ccgccctgag agcaggattt caccgctgtc tggaaatgtt tgtagaacgg
    16081 gatacagccg gtatggaacg cttcgccacc gcgaatcggg ctgcccagcg cacgaatacg
    16141 tccggcgttg atgccgatcc cggcacgctg ggaaacatat ttcacaatcg cgctggaggt
    16201 ggcgttgatg gaatccaggc tgtcaccgca ctcgatcagt acgcaggagc tgaactgacg
    16261 agtcggggta cgcacgccgg acatgattgg cgtcggcagc gaaattttaa atgtggaaac
    16321 cgcgtcataa aaacgcttca cgtattgcag gcgcgtttca cgcgggtagt tcgagaacag
    16381 gcacgcggca acaagaatat aaaggaactg ggcgctttcg tagatttcgc cggtcacgcg
    16441 gttctgtact agatatttgc cttccagctg cttaacggca gcataagaga aggtcatatc
    16501 acggtcgtga tcgataaacg tgtccatctg cttgaactct tcttccgtgt agtcttccag
    16561 cagatgatta tcgtatttgc ccatctcgac cattttcacc acgtggtcgt acagcgcagg
    16621 cggctcaaac tggccgtagg cttttttacg caggtggaag atcgccaggc gcgcggcgag
    16681 atactgataa tccggcgcat cacgggagat caggtctgcg gcagccttga taatagtttc
    16741 gtggatgtca gaggtcttga taccgtcata aaactgaatg tgggagcgca gctcgacctg
    16801 ggaaatcgaa acgttatgca gtccttctgc cgcccaatcc agaacgcgat ggattttgtc
    16861 gagattgatg cgctctgtgc taccgtcgcg ctttgtcacc agcagattct gattcatgta
    16921 tgtcgtacct gtttttggaa ttctttccgc aatagtttca tgcagactac ctgtagtaag
    16981 ggaagctgct tagaacacta tatatagggg gtatgtttgg gattcactgc aagatagtgt
    17041 gaaaatgacc ctcttgcaag tgcataactt tgtggataac tcaggaagga aaaagtggct
    17101 ttcgcgcacc ttaggtcaga caaggtgtcc gggaaagtca atgggaagag aaaaatttgt
    17161 taaaaataac tgttcggcga tttcttgaac ggcagaatag gttggataaa ttgatataga
    17221 tggtttatta atctttttgc taatgattgc aaaaattgat ctaagcacga gtcatcacat
    17281 aatctgatgt atcagttgtg gtcaatatat aagcgaatgt aagcgattgt tgatgtcgaa
    17341 atggttgtt
//
LOCUS       DAZPCS010000079        17292 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZPCS010000079 DAZPCS010000000
VERSION     DAZPCS010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17292
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.079"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            231..1664
                     /gene="cscA"
                     /locus_tag="ACHUQ7_002462"
     CDS             231..1664
                     /gene="cscA"
                     /locus_tag="ACHUQ7_002462"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFR1135829.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALALGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWAFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRRISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLILWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1672..2667)
                     /locus_tag="ACHUQ7_002463"
     CDS             complement(1672..2667)
                     /locus_tag="ACHUQ7_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR1135830.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGRVTRIPCPLLIRCST"
     gene            2898..3116
                     /locus_tag="ACHUQ7_002464"
                     /pseudo
     CDS             2898..3116
                     /locus_tag="ACHUQ7_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3134..4462
                     /gene="dsdA"
                     /locus_tag="ACHUQ7_002465"
     CDS             3134..4462
                     /gene="dsdA"
                     /locus_tag="ACHUQ7_002465"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFR1135831.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPSTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASCFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="ACHUQ7_002466"
     CDS             complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="ACHUQ7_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFR1135832.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPRLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="ACHUQ7_002467"
     CDS             complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="ACHUQ7_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFR1135833.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7687..8301
                     /gene="evgA"
                     /locus_tag="ACHUQ7_002468"
     CDS             7687..8301
                     /gene="evgA"
                     /locus_tag="ACHUQ7_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFR1135834.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8306..11899
                     /gene="evgS"
                     /locus_tag="ACHUQ7_002469"
     CDS             8306..11899
                     /gene="evgS"
                     /locus_tag="ACHUQ7_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFR1135835.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKIKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="ACHUQ7_002470"
     CDS             complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="ACHUQ7_002470"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFR1135836.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLYNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     LGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="ACHUQ7_002471"
     CDS             complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="ACHUQ7_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFR1135837.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="ACHUQ7_002472"
     CDS             complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="ACHUQ7_002472"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFR1135838.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPIIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15936..17186)
                     /gene="frc"
                     /locus_tag="ACHUQ7_002473"
     CDS             complement(15936..17186)
                     /gene="frc"
                     /locus_tag="ACHUQ7_002473"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFR1135839.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
BASE COUNT         4991 a         3974 c         3724 g         4603 t
ORIGIN      
        1 tactttggct gacatttttt tcccttaaat tcatctgagt tacgcatagt gataaacctc
       61 tttctcgcaa aatcgtcatg gatttactaa aacatgcata ttcgatcaca aaacgccata
      121 gttaacgtta acatttgcga tattcatcgc atttatgaaa gtaagggact atatttttat
      181 aaaagttaac gttaataatt caccaaattt gcttaaccag gatgattaaa atgacgcaat
      241 ctcgattgca tgcggcgcaa aacgcactag caaaacttca tgagcaccgg ggtaacactt
      301 tctatcccca ttttcacctc gcgcctcctg ccggatggat gaacgatcca aacggcctga
      361 tctggtttaa cgatcgttat cacgcgtttt atcaacatca cccgatgagc gaacactggg
      421 gaccaatgca ctggggacat gccaccagtg acgatatgat ccactggcag catgagccta
      481 ttgcgctagc gctaggagac gataatgaca aagacgggtg tttttcaggt agtgctgtcg
      541 atgacaatgg tgtcctctca cttatctaca ccggacacgt ctggctcgat ggtgcaggta
      601 atgacgatgc aattcgcgaa gtacaatgtc tggctaccag tcgggatggt attcatttcg
      661 agaaacaggg tgtgatcctc actccaccag aaggaatcat gcacttccgc gatcctaaag
      721 tgtggcgtga agccgacaca tggtggatgg tagtcggggc gaaagatcca ggcaacacgg
      781 ggcagatcct gctttatcgc ggcagttcat tgcgtgaatg ggccttcgat cgcgtactgg
      841 cccacgctga tgcgggtgaa agctatatgt gggaatgtcc ggactttttc agccttggcg
      901 atcagcatta tctgatgttt tccccgcagg gaatgaatgc cgagggatac agttaccgaa
      961 atcgctttca aagtggcgta atacccggaa tgtggtcgcc aggacgactt tttgcacaat
     1021 ccgggcattt tactgaactt gataacgggc atgactttta tgcaccacaa agctttttag
     1081 cgaaggatgg tcggcgtatt gttatcggct ggatggatat gtgggaatcg ccaatgccct
     1141 caaaacgtga aggatgggca ggctgcatga cgctggcgcg cgagctatca gagagcaatg
     1201 gcaaacttct acaacgcccg gttcacgaag ctgagtcgtt acgccagcag catcaatctg
     1261 tctctccccg cagaatcagc aataaatatg ttttgcagga aaacgcgcaa gcagttgaga
     1321 ttcagttgca gtgggcgctg aagaacagtg atgccgaaca ttacggatta cagctcggca
     1381 ctggaatgcg gctgtatatt gataaccaat ctgagcgact tattttgtgg cggtattacc
     1441 cacacgagaa tttagacggc taccgtagta ttcccctccc gcagcgtgac acgctcgccc
     1501 taaggatatt tatcgataca tcatccgtgg aagtatttat taacgacggg gaagcggtga
     1561 tgagtagtcg aatctatccg cagccagaag aacgggaact gtcgctttat gcctcccacg
     1621 gagtggctgt gctgcaacat ggagcactct ggctactggg ttaacataat atcaggtgga
     1681 acaacggatc aacagcgggc aagggatccg cgtcactctt cccccttcac gaccttcaat
     1741 aatatgcaat gcagcttccc gcccgataat gtcatgtgga agctgaattg tggtcagcgg
     1801 cggtaaaaac agatgcccga cgccaaccag attatcaaag cccattacag cgacatcctg
     1861 cgggatacgt acccccttcg ccaaaagaac ctgataagcc acaaaggctg cgcgatcgtt
     1921 accacatatc agaacatcaa aatctggttt gcccgatttg aagtgagcat tgagtaaact
     1981 tgcgagatcg gtgtagtgat catcacctgt tgccatgtga aattgtttca cctcagccag
     2041 atctcgtcca gcatcacgcc aagcctgctc aaatccctgc cgacgatacc ctgttgccaa
     2101 cgcactttcc ggtagccaga agcataatgg ttgacgatag cccgccgcga gcaaatgctg
     2161 tgttgattca tattgtgcag tgtaatcatc agggatataa ctgggtaacg ctgggtcatc
     2221 cgccacacag ttcgccaata caatattttc accatacaga gactcaggca gcgtgatatg
     2281 tcgcagcccc attgtagtat agataatgcc atccggacgg tgggcaagca gctgacgtgc
     2341 cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa aaactattcc agccgaactc
     2401 gctggcggtt tgctcaatgg caagcagaat atcaacagag aaaggagtgg tagccgtgtc
     2461 ctgcgccagc acggcgagag tcgacggctt acgtccttga gcgcgcatct tacgggcgga
     2521 aagatcagga acataattca gggtctggat tgcctgcaat acgcggtcac gcgttgcagg
     2581 acgcacagat tctgcattat gcatcacccg ggagactgtc atcatcgaca ctcccgccag
     2641 gcgtgcgaca tcctttaatg aagccatacc caagccgttt gccgtaaaac gggcactgta
     2701 gcagaaacag acgtcactgg cgagatccaa cgccctatca cctgacacag caatacaata
     2761 aaaaataaca ataattcccg gacaattgtc cccaattccg cctctgttct cgccaacgag
     2821 taaccatata ggcaagtaag taaaacatgc caactggcat cgccaacctg gagaagaaaa
     2881 tataaatatg gatcattgtg ctgccactgt atcccgacat cagcccggaa attattgcga
     2941 ttgctatcgg ttcaggtgca attggctgca cgatcgttac ggactcgctt ttctggctgg
     3001 tgaagcaata ttgcggcgct acgctcaatg aaacatttaa atactatacg acagcgacat
     3061 ttatcgcttc agtcgtcgct ctggcgggca cattcctgct gtcatttatc atctaagcgc
     3121 aaagagacgt actatggaaa acgctaaaat gaactcgctc atcgcccagt atccgttggt
     3181 aaaggatctg gttgctctta aagaaaccac ctggtttaat cctagcacga cctcattggc
     3241 tgaaggttta ccttatgttg gcctgaccga acaggatgtt caggacgccc atgcgcgctt
     3301 atcccgtttt gcaccctatc tggcaaaagc atttgctgaa acagctgcca ctggggggat
     3361 tattgaatca gaactggttg ccattccggc tatgcaaaaa cggctggaaa aggaatatca
     3421 acaaccgatc agcgggcaac tcctactgaa aaaagatagc catttgccca tttccggctc
     3481 tataaaagca cgcggcggga tttatgaagt cctggcacat gcagaaaaac tggctctgga
     3541 agcggggttg ctgacgcttg atgatgacta cagcaaactg ctttctccgg agtttaaaca
     3601 gttctttagc caatacagca ttgctgtggg ctcaaccgga aatctggggt tatcaatcgg
     3661 cattatgagc gcccgcattg gctttaaggt gacagttcat atgtctgctg atgcccgggc
     3721 atggaaaaaa gcgaaactgc gcagccatgg cgttacggtc gtggaatatg agcaagatta
     3781 tggtgttgcc gtcgaggaag gacgtaaagc agcgcagtct gacccgaact gtttctttat
     3841 tgatgacgaa aattcccgca cgttgttcct tgggtattcc gttgctggcc agcgtcttaa
     3901 agcgcaattt gcccagcaag gccgtatcgt cgatgctgat aaccctctgt ttgtctatct
     3961 gccgtgtggt gttggcggtg gtcctggtgg cgtcgcattc gggcttaaac tggcgtttgg
     4021 cgatcatgtt cactgctttt ttgccgaacc aacgcactcc ccttgtatgt tgttaggcgt
     4081 ccatacagga ttacacgatc agatttctgt tcaggatatt ggtatcgaca accttaccgc
     4141 agcggatggc cttgcagttg gtcgcgcatc atgctttgtc gggcgggcaa tggagcgtct
     4201 gctggatggc ttctataccc ttagcgatca aaccatgtat gacatgcttg gctggctggc
     4261 gcaggaagaa ggtattcgtc ttgaaccttc agcactggcg ggtatggccg gacctcagcg
     4321 cgtgtgtgca tcagtaagtt accaacagat gcacggtttc agcgcagaac aattgcgtaa
     4381 tgccactcat ctggtgtggg cgacgggagg tggaatggtg ccggaagaag agatgaatca
     4441 atatctggca aaaggccgtt aataacgttt caacgcaaca tcgcaatcct ttccctgggt
     4501 gagcgatgct gccgatggcg cagacttaag atccccggtc ttacccgcta taaccccctc
     4561 cttttatcat cacccaacgc ctttcgctgt aaacggcggt ttcgcaaacc aaaccaaaac
     4621 cgtaagtaag ataaaagcaa tagccgccat acggaaaatc tcatttgcag aaatagaaag
     4681 tgactgctgg gttatttcat tattaatttc attaagaact cctgaaagcg aaccatagta
     4741 tttatccata atttgtgacg atgaattaaa cacggggtta aattgatcga tggttgctgt
     4801 caactgacta tggtgtaacg attcgcgtcg tccccacagc gtcattgtca acgacgtacc
     4861 aactgatcct gacaaggtac gaaaaaaatt actcatactc gaggcattgg caaatttatt
     4921 atctggcaag cctgaaaacg aaatcgttgt taagggtaaa aagaaacagg caacggcgaa
     4981 tccctggaaa aactgtggca aaatgatgcc tgtaaaatca atcgttggca taaatgtcac
     5041 agaacgccag taatagcaaa ccgcatacat caaaaaactg aatgtcacta acacccgcat
     5101 gtctatttta ttgccataac gtcctatcaa aggtgaaatc aatagtggca tgatgccgat
     5161 gggcgcataa gcaagcccgg cccatatcgc attatacccc atcgttttct ggagtaactg
     5221 cggcataagg acgatcgctc cagagtaaaa taaatacgcg caggtgatac tcacaatacc
     5281 aatggtgaaa ttacgggact taaacaaact gagatcaaga atcgggttct ctgaggtcga
     5341 ttcccaaatg actaaagaga tcagagaaat aactgatact actgttaata ttattattgt
     5401 actcgagttg aaccaatcca gatcgcgccc tttatcaagc ataatttgca agccaccaac
     5461 accgagcact aacagggtca gtcttggtag attcattttt accggtgaag tttcagtttc
     5521 tcttccttta agtaaggtta agcataatgt caggacgata atccccatag ggacattgat
     5581 taaaaatatc caaccccagc taaagttatc gcaaatatag ccgcccaata tcggcccaca
     5641 tatcggagcg ataatcacgg tcattgacca taatgccaga gcaaatgttc ttttttctgg
     5701 tggataattc cttaataata aactctgtga cagtggaatt aacggccccg ccattaatcc
     5761 ctgaacgact ctaaaaaata tcagcacatc aagattggtc gataaactac acattaagga
     5821 agacagcgaa aaaaaagtga ctgaaagtaa aaataacctt aattcgccta ttctttgtgc
     5881 cagcctgccg gtaacaggga tcgcaatggc atttgctacg ccaaacgagg tgataaccca
     5941 ggtgccttcg tctgttgatg ctcccagaaa gccagatatt gtcggtattg cgacgttaga
     6001 aatagtggaa tccaacattt gcataaacgt cgctaatgac aatgcaatag taacgcacca
     6061 taacgtccca ccggttaatg gtgccggagt tgatttagtg attgccatta aagttgtcca
     6121 ttatgcgaaa taatgttgct aatttctttt tctatcggac tggtatcgat aactaaagcc
     6181 ttactggtat aagcaggcat ggaggtcacg gttgaagcca gatcaggcat ctcagcaatg
     6241 tcttcgtcct tcgtatcaat agttgcagtc atcgataaac caatgcgcaa ggggtgttcc
     6301 atgagttctt ttggatcaag agaaacttca accggtacac gctgaacgat tttgatccag
     6361 ttccctgtcg cattttgtgc aggtaataag gagaacgcat tgccggttcc catattgatc
     6421 cctgtcaccc gaccatgaaa cacaacattt tcaccataaa gatcgctgat aatattgact
     6481 gattgaccaa tccgtacatc cgtgagttgt gtttctttaa agttggcatt aacccacatt
     6541 tgacgtgccg gtacgacagc cattaacgat tgtccggaac tcactgtttc gccgacctga
     6601 acacttcgct gggcaatata gccagtaacc ggactcctta tatccgtacg tttaagcacc
     6661 aaccaggctt ctttggttgc atccgccgct tcaactactt gtggctgacg gtttaatggt
     6721 gtgttcatta ctaaagcttt attcgcttta taagcctgga tagcggcatt cagtgccgct
     6781 ttgctactta ttaacgtatc tttggtatgc tccagcgttt cttttgaaat aaccccctgc
     6841 ttcgctaaag gcacacgacg gttataatct tctaaagatt gttgatactg aatacgtgct
     6901 gaagcgactt cggcactgta ttgtttatcc tgtaagtaca gtttattcgt ttgccgaaca
     6961 atatttgcca gattattttt agctttattg agtgcgatag tggcatcagt tttatccagt
     7021 gaaactaaaa tgtcaccctg tcgaacgtag ttcgtatctt tatgattaac tacagtgaca
     7081 ctacctgaga cttgtgcaga aattggatct gcattccccg tgacataggc gtcatctgta
     7141 ctaatcatgt cttctaattc cattgaccaa taggcatagg cacctgaaaa cgcaataaat
     7201 aaaactatca ccaataaaga aaagtatttt tttctgttag actgtttttt atttgaatta
     7261 atctgttcca ctattatctc tcatttcgca tagatgaaat ttatgaattg aatgaaaaga
     7321 gtattcattc ttcatatcaa acacagtgca tgtgttgcac attatcccag gaacgttgga
     7381 ggcctaattt aaatgaggtg caaaaaaaac aacagaggac taaaccgtgg cttttgcaat
     7441 acaattctta cgcctgtagg attagtgaga agacttatag tgctaacttg aaactataaa
     7501 tcatcggtat aatccctgat tttattgttg acatttcatt tatgccgact atttatatgg
     7561 tatacttgtc gaattatctt aaaggaaact cagattttct tatttttatt gagaaaatga
     7621 gatgacgcct tatgtctgta ttactacagg gagaagggag atgcttcatt gcaaagggaa
     7681 taatctatga acgcaataat tattgatgac catcctcttg ctatcgcagc aattcgtaat
     7741 ttattgatca aaaacgatat tgaaatctta gcggagttaa ctgaaggcgg aagcgccgtt
     7801 cagcgggtgg aaacacttaa gcctgatatc gtcatcattg atgtcgatat ccccggagtt
     7861 aacggtatcc aggtgttaga aacgctgagg aagcgccaat atagcggaat tattattatc
     7921 gtctccgcta aaaatgacca tttttacggg aaacattgtg ctgatgctgg cgctaatggt
     7981 ttcgtgagta aaaaagaagg catgaacaat atcattgcgg ctattgaagc tgcaaaaaat
     8041 ggctactgct atttcccctt ctctctcaac cggtttgttg gaagtttaac gtccgaccag
     8101 caaaaactcg actccttatc gaaacaagaa attagtgtca tgcggtatat tcttgatggc
     8161 aaggataata atgacattgc ggaaaaaatg ttcatcagca acaaaactgt cagcacgtat
     8221 aaaagtcgcc tgatggaaaa attagaatgt aaatcactga tggatcttta cacattcgcg
     8281 caacgtaaca aaatcggcta atcacatgaa gtttttaccc tatttttttc ttctctgttg
     8341 tggtctttgg tcgaccataa gtttcgcaga cgaagattac atcgaatatc gtggcatcag
     8401 tagtaacaac cgtgtcacac ttgatccagt acgtctgagc aacaaggaat tacgttggtt
     8461 agcgagcaaa aaaaatcttg tgattgcagt acacaagtcc caaacggcta ccttgttgca
     8521 taccgattcg cagcaacagg ttcgtggtat taatgctgat tatttaaatc ttttaaaaag
     8581 agcgttaaat atcaaattaa cactccggga atacgcagat catcaaaaag caatggacgc
     8641 gcttgcagaa ggtgaagtcg atatagtgtt atcacattta gttacttcgc cgcctcttaa
     8701 taatgacatt gctgcaacca aaccattgat aattaccttt ccggcgctgg taaccaccct
     8761 tcacgactca atgcgaccgc ttacctcacc aaaaccagta aatattgctc gggtagcaaa
     8821 ttacccccca gacgaggtaa ttcatcaatc atttccaaaa gcaacaatta tctcttttac
     8881 aaatttatat caggcattag catccgtctc agctgggcac aatgattact ttattggtag
     8941 taacatcatt accagcagta tgatttcccg ctatttcact cactccttaa atgtagtgaa
     9001 atattataac tcgccgcgtc aatataattt tttcttgacc agaaaagaat ctgtcattct
     9061 taatgaagta ctcaatagat ttgttgatgc tttaacaaat gaagttcgct atgaagtatc
     9121 acaaaattgg cttgatacag gaaacctggc ctttctgaac aaaccattag aactcactga
     9181 acatgaaaaa cagtggatta agcagcatcc caatttaaag gtgctggaaa atccttactc
     9241 gcccccctat tctatgacgg atgaaaatgg ctcggttcgg ggcgttatgg gggacattct
     9301 taatattatt accttgcaaa caggtttaaa tttttctccg atcaccgttt cacacaatat
     9361 ccatgctgga acacaactta gccccggagg atgggatata atacctggcg ctatttatag
     9421 tgaagatcga gaaaataatg ttttatttgc tgaagccttc ataacaacgc cttacgtttt
     9481 tgtcatgcaa aaagcgcctg acagtgaaca aacattaaaa aaaggaatga aagttgccat
     9541 tccatattat tatgagctgc attcgcaatt aaaagagatg tatccggagg ttgaatggat
     9601 acaggtcgat aatgccagcg ctgcatttca caagattaag gaaggtgaac ttgatgctct
     9661 ggtcgcgaca cagctaaatt cgcgttacat gatcgatcat tactatccta atgaacttta
     9721 tcattttctt attcctggcg ttccgaatgc atcgctttcg ttcgcttttc ctcgcggaga
     9781 accggaactt aaggatatta ttaataaagc actgaatgca attcccccaa gcgaagttct
     9841 gcgcctgacg gaaaaatgga ttaaaatgcc caatgtgacc attgacacat gggacctata
     9901 tagcgagcaa ttttatattg ttacgacatt atccgtttta ttagttggca gtagcctttt
     9961 atggggattc tacctgttac gctcagttcg tcgtcgtaaa gtcattcagg gtgatttaga
    10021 aaaccaaata tcattccgaa aagcactctc ggattcctta ccgaatccaa cttatgttgt
    10081 aaactggcaa ggtaatgtca ttagtcataa tagtgctttt gaacattatt tcactgcgga
    10141 ttactacaaa aatgcaatgt taccattaga aaacagtgat tcacccttta aagatgtttt
    10201 ttctaatgcg catgaagtca cagcagaaac gaaagaaaat cgaacaatat acacacaggt
    10261 atttgaaatt gataatggca tcgagaaaag atgcattaat cactggcata cattatgcaa
    10321 tcttcctgca agtgacaatg cagtatatat ttgtggttgg caagatatta ctgaaacgcg
    10381 tgatctaatt aatgcactcg aggtagaaaa aaataaagcg ataaaggcta ccgtagcaaa
    10441 aagtcagttt ctggcaacga tgagtcacga aataagaaca ccaataagct ctattatggg
    10501 cttcctggaa cttctgtcgg gttctggtct tagcaaggag caacgggtgg aggcgatttc
    10561 acttgcctac gccaccggac aatcactcct cggcttaatt ggtgaaatcc ttgatgtcga
    10621 caaaattgaa tcgggtaact atcaacttca accacaatgg gtcgatatcc ctactttagt
    10681 ccagaacact tgtcactctt tcggtgcgat tgctgcaagc aaatcgatcg cattaagttg
    10741 cagcagtaca tttcctgatc attacctggt taagatcgac cctcaggcgt ttaagcaggt
    10801 cttatcaaat ttactgagta atgctctcaa atttaccacc gagggggcag taaaaattac
    10861 gacctccctg ggtcacattg atgacaacca cgctgttatc aaaatgacga ttatggattc
    10921 tggaagtgga ttatcgcagg aagaacaaca acaactgttt aagcgctaca gccaaacaag
    10981 tgcaggtcgt cagcaaacag gttctggttt aggcttaatg atctgcaaag aattaattaa
    11041 aaatatgcag ggcgatttat cattagaaag tcatccaggc ataggaacaa catttacgat
    11101 cacaatcccg gtagaaatta gccagcaagt ggcgactgtc gaggcaaaag cagaacaacc
    11161 catcacacta cctgaaaagt tgagcatatt aatcgcggat gatcatccga ccaacaggct
    11221 attactcaaa cgccagctaa atctattagg atatgatgtt gatgaagcca ctgatggtgt
    11281 gcaagcgcta cacaaagtca gtatgcaaca ttatgatctg cttattactg acgttaatat
    11341 gccgaatatg gatggttttg agttgactcg caaactccgt gagcaaaatt cttccttacc
    11401 catctggggg cttacagcca acgcacaggc taacgaacgt gaaaaagggt taagttgcgg
    11461 catgaactta tgtttgttca aaccgttgac cctggatgta ctgaaaacac atttaagtca
    11521 gttacaccag gttgcgcata ttgcacctca gtatcgccac cttgatatcg aagccctgaa
    11581 aaataatacg gcgaatgatc tacaactgat gcaggagatt ctcatgactt tccagcatga
    11641 aacgcataaa gatctacccg ctgcgtttca tgcactagaa gctggcgata acagaacttt
    11701 ccatcagtgt attcatcgca tccacggtgc ggctaacatc ctgaatttgc aaaagttgat
    11761 taatattagc catcagttag aaataacacc tgcttcagat gacagtaagc ctgaaattct
    11821 tcagctgctg aactctgtta aagaacacat tgcagagctg gaccaggaga ttgctgtttt
    11881 ctgtcagaaa aatgactaaa ttgcggctcc cacaatgttc taatgtggga gctatttacc
    11941 agcacatctt acatttatga tgagaactcc tggcgaattt gttccccatg ctggtcgagc
    12001 gttgccgctc ccggcataac atgcgggtcc gcgcagccgc tgatttttat cggattaccc
    12061 ggcatcatta ttcctccggc ttcaatcaac atatttctcg cctgagtttg tggcaaatta
    12121 atggcctcag ccacacttaa taacggcgcg acgggcacac caacttcatg tattttggct
    12181 aaccaaactt cagctgcctg cgtttttaac gttcgctcaa tatattgttt aagaatagcc
    12241 tggttttgta cgcgtaaaat attgctgcta aatcggggat cattaaccag ttccgtaagc
    12301 tccagtgcct ggcataacgc agaaaaaagc ttgtcattac cacaacaaat cgtaatcggc
    12361 ttatcctgag tatcgaaaac atcaaaaggt gccatgtagg gatggcgatt tcccaggcgt
    12421 tgtggtgact tccctgtcgc aatatatgcc atcagaccat gctccagaaa actcagcgtg
    12481 gcatcaaaca tcgctatatc gacatgcgcc cctctctggc tcttttcgcg gccataaagt
    12541 gcactcacta ttccgctgaa taaatagaca ccgccgcata gatccgcaag agaggtacca
    12601 acacgcactg gcggagcatc agggtaccct gtttccatca ttatcccact cattgcctga
    12661 atgatggtat cgtaggcagg agcatctttt agcggaccgg tatgtccgaa acctgacgat
    12721 gaagcatata tgaggcgcgg gttgatttct tgtaaccttt cccatgaaaa ccccagtttt
    12781 tccattgtac ctaggcgaaa attctcagct aatacatcag cttgtttgag catatttata
    12841 aatatacttt tatcgtgatc attctttaaa tcaagaacca cactctcttt gccatgatta
    12901 ataaaactgt aatagagtga ctgtccatcc acatagggac caaatgtgcg ggtatcatca
    12961 ccatgacctg gtggctcaac tttaattacc cttgcgccca tattataaag aagttgagtt
    13021 ccgaaaggtc cattaaggac atgtgtcata tcgataacta ataagccttc aaacggccct
    13081 ttgctttcat tatttgtcat ttgcttatgc cttgaaaaaa gccgaaggtg aaaagacact
    13141 tcggcattta tgtaaaatca ataacatgat taattatgaa accagtcgac tgacataaat
    13201 ccacaaggga gccgtgacga caaagcccag tacgctcaca gccaatgacg ctgtaccagt
    13261 acgggtgtag acattaaacc ggctggcaat aatgattccg gagaatgccg gcggtaatgc
    13321 gcctgccagt accatcatct gcaaatgttc gctgttcaaa tgacatgcca taccaacgag
    13381 aagcagcgcc agtggcatca gaatcaactt caggaaggtg ttataagcaa tttcggcact
    13441 gaactcgaat ttatgtgcag ccagagtcaa ccccgcagcg aataccgcta cccctgagtt
    13501 agccttcgca atcagattaa aggttgggtc ccatgctgcc ggaattttta ccccaaccag
    13561 caccaggatc gttgccagaa caggtgccca tactactggc tcctttgccg cagaaattaa
    13621 tgcgctcaga ttactattct tcttaccatc cgctcctgag gaaggattca gcaaatacag
    13681 accaatagga atagtaattg cgttaacaat aatagaaata attgctacca ctaaacctgt
    13741 tgatacggaa tcaccataaa taggatcgag aactgcaaac cccaggaatc caatggtagg
    13801 tgaacccgca attaatgcac atacagctgc ttctgcatgg gtacgtttaa aaaatttgta
    13861 gcaaccaaac caggagaaaa agaaacatcc gacaataacc acaagtgata caagggtcag
    13921 acgagtgtcc gcaaaaatca tttccctgtt tgcccgggta atagaaacaa atagagccgc
    13981 aggaagagcg tagtttaata ccagtttatt aaatgcgcgg gcttgatctt ctgaaaaagt
    14041 ttctcgtctg ccgctaaaat aacccaacag catgattacg ataataggca ataaatcgcc
    14101 aataaaaaat gttagcataa tagtatttcc gataagggtc ccctcaccat cgcataatga
    14161 gttggcaaga ggagacgatg tcagtattta attaccagcg acttgttttg ggttaagttt
    14221 ggtaatatgg ccactttcag ttcctgctgc agggtcgata accacattaa taatggtcgg
    14281 tttgcgcgac tgaataccgg tggttaaagc atgacgaagt tcatctgtcg tggtgacgtt
    14341 atagccaacg ccacgaaacg catccattaa tttgtcatac cttgcatggt gcaacagatc
    14401 cgttggtgat ggtgcaccag cgccactgag atcaacaccg tctcctctgt agatgccgcc
    14461 attattaaaa ataacgatcg tcaccggcag gttatatcga caaatcgttt caatttccat
    14521 cccactgaaa ccaaaagcac tatcaccttc aatggcgaca accggagaac cagaagtcac
    14581 gctagcaccg atggcatagc ccataccgat gcccatgaca ccccaggtac cacaatccag
    14641 acgacgacgt ggtttataca tatcaataat atttcgtgca ttatccaggg tatttgcacc
    14701 ttcattaact aaataaatat cctggttctc gcgcaatacg tcgcgcacag cacttaatgc
    14761 attaaagtaa tttaatggtt gagtatctgt acttaatttt tcatgcattt tttgtgcatt
    14821 ttgctgcttg tggatattta aaatatcgcg ccataccagt ggagtcgtaa atgtgttttg
    14881 tttcagttct gccagcatac cttgcatact ggatgcaata tcaccaacga ctggcacagc
    14941 aatggggcgg ttgctgtcaa tttcctgcgg ttcaatatcc agttgaataa actgtgtatc
    15001 tgccgcccat ccttttttac cgtgtgccaa taaccaattc agtcgtgcac caacaagcat
    15061 gacaacgtca gcatttgcca gggcaaacga acgcgcagct gccgcagaaa gtggatgcgt
    15121 atcttcaagg atccctttcg ccatagacat tggcaggaat ggaatctgag cactttcaat
    15181 aaattcacga agctgttcat cagcttgtga atacgccgcg cctttgccaa ggataattaa
    15241 tggccgttca gcttttgcta aaagcgaaat tgcgctagtg actgacttcg ggcatggcaa
    15301 taatgctggc gacggatttt caactttaac aatcgtggtt aacgcttcgt ctttttccat
    15361 cgtcgcggcc aggacatttg ctggcaaatc aagataaact ccgccagggc gacccgatac
    15421 agagacccgg atagcgcgtg ccaatgcaat gccaagatcc tgcggctgat taacgcgaaa
    15481 tgctgctttg gcatacggtt ttgccgcatt catttggtcc agctcttcat aatcaccttg
    15541 ctgtaggtcg acgatcgcgc ggtcgctgga gccgctaatc atgataatcg gaaaaccatt
    15601 taccgttgcg ttggccaatg cggtcaaacc attgaggaat cctggcgcag aaactgtcag
    15661 gcagatcccc ggtttttggg taagaaaacc gcttgccgca gcggcatagc ctgccgactg
    15721 ctcatgacga aaaccaatat aacgaatgcc ttccgcctgg gcatggcgtg ccatatccgt
    15781 cacaggaata cctacaacac cataaatagt gtcaatatta ttctgtttta atgcttcaac
    15841 gatgatatgc ataccatctg tcatttgaag ttgatctgac atgattaata acctcgaaat
    15901 ttttataaat tgggagccag aagcccccgt taatatcaga tggcgtggtt ttgcttcatt
    15961 gcagcaattt catcgtcgct ataacccagc tcctgcaata cagcagcggt atgttcacct
    16021 aatagcggcg cagctttaat atccggcgta aaggcagaga atttcattgg acaaccaacg
    16081 gtcagatatt ttccacgcaa cggttgttcc acttcaacaa cactgccact ttggcgcaaa
    16141 gagggatcaa gtgaaatttc tttcatactt aaaaccggtg cacaaggaat atcaaactga
    16201 gtcaaatagg ccaccgcttc atgtttatca atagtgacag tgtatttttc gatttcagca
    16261 aaaatatcga aaatatgtgg ctgtcgggca tgggctgtac tgtatgccgg atcggtaatc
    16321 cattctggtt tgccgatggc tttacaggtg ttttcccagt tttgctcctg aatagtgaaa
    16381 taaatatagg cgttaggatc ggtttcccag cctttacatt tcaggatcca gccaggctga
    16441 ccgccaccac ctgcattacc accgcgggga actgcatcac caaatgtacc attcggatac
    16501 tgcgggtatt cttccagata acccaattta tcgagacgct gctggtcacg taatttcacg
    16561 cggcaaaggt tcaatacggc atcctgcatt gacatggtga ctcgttgccc acgccccgtt
    16621 ttttcgcgat gcagcaaagc agcaagtaaa ccgatcagca aatgcattcc ggtgttgctg
    16681 tcacccaacg ctgcagcgct taccagcggc ggaccatccc aaaaacccgt agtggatgcc
    16741 gcgccacccg ctgcctgagc aacgttttca taggctttta cattcacata aggcgaacac
    16801 tcatcaaacc ctttgatcga accaaaaatc agacgtggat tgatttcttg aatatgctcc
    16861 caggtgaagc ccatgtgatc aatggcccct ggatgaaagt tctcgactaa gatatcagct
    16921 tcgcggatca gcttttccat tacctctttg ccttccgctg ttttggtatt taactcaata
    16981 gaacgtttgt tactgttaag catggtgaag taaagcgcat cgatatcagg aatatctcgc
    17041 agttggtgac gcgttacgtc accaacgccg ggacgttcaa ttttaataac gtcagcgcca
    17101 aaccaggcca gcatttgagt acaagatggg ccagattgca caccggtgaa atcgagaact
    17161 ttaattcctt gaagtggagt tgacatagca atacctatta gttaattaaa tatgtgttaa
    17221 tttagcgttt atttaaggca taacgccctc tataaataat ctgcaaaata tcttcgtagc
    17281 ggcactcgat at
//
LOCUS       DAZPCS010000080        17144 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZPCS010000080 DAZPCS010000000
VERSION     DAZPCS010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17144
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.080"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..510
                     /locus_tag="ACHUQ7_002474"
     CDS             <1..510
                     /locus_tag="ACHUQ7_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFR1135840.1"
                     /translation="PFREKPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDG
                     FTQPIVVTHTDKNAMEIVDGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATI
                     RHNRARGRHQITAMSEIVRELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADR
                     QYSRAWTVK"
     gene            complement(511..1671)
                     /gene="ybdL"
                     /locus_tag="ACHUQ7_002475"
     CDS             complement(511..1671)
                     /gene="ybdL"
                     /locus_tag="ACHUQ7_002475"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFR1135841.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            1780..2868
                     /gene="hcxA"
                     /locus_tag="ACHUQ7_002476"
     CDS             1780..2868
                     /gene="hcxA"
                     /locus_tag="ACHUQ7_002476"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFR1135842.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHNFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(2878..3075)
                     /locus_tag="ACHUQ7_002477"
     CDS             complement(2878..3075)
                     /locus_tag="ACHUQ7_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFR1135843.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(3257..5362)
                     /gene="cstA"
                     /locus_tag="ACHUQ7_002478"
     CDS             complement(3257..5362)
                     /gene="cstA"
                     /locus_tag="ACHUQ7_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFR1135844.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQSVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTADWLKAFSPDAKVGFLVIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(5543..5956)
                     /gene="entH"
                     /locus_tag="ACHUQ7_002479"
     CDS             complement(5543..5956)
                     /gene="entH"
                     /locus_tag="ACHUQ7_002479"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFR1135845.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTLLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(5959..6705)
                     /gene="entA"
                     /locus_tag="ACHUQ7_002480"
     CDS             complement(5959..6705)
                     /gene="entA"
                     /locus_tag="ACHUQ7_002480"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFR1135846.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALTFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTARIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(6705..7562)
                     /gene="entB"
                     /locus_tag="ACHUQ7_002481"
     CDS             complement(6705..7562)
                     /gene="entB"
                     /locus_tag="ACHUQ7_002481"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFR1135847.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(7576..9186)
                     /gene="entE"
                     /locus_tag="ACHUQ7_002482"
     CDS             complement(7576..9186)
                     /gene="entE"
                     /locus_tag="ACHUQ7_002482"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFR1135848.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSAEIC
                     QFTQQTRYLCSIPAAHNYTMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEDIENL
                     LLSHPAVIYAALVSMENELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(9196..10371)
                     /gene="entC"
                     /locus_tag="ACHUQ7_002483"
                     /pseudo
     CDS             complement(9196..10371)
                     /gene="entC"
                     /locus_tag="ACHUQ7_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
     gene            10663..11619
                     /gene="fepB"
                     /locus_tag="ACHUQ7_002484"
     CDS             10663..11619
                     /gene="fepB"
                     /locus_tag="ACHUQ7_002484"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFR1135849.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(11623..12855)
                     /gene="entS"
                     /locus_tag="ACHUQ7_002485"
     CDS             complement(11623..12855)
                     /gene="entS"
                     /locus_tag="ACHUQ7_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFR1135850.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATSALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPELPPPPQPLEHPLKSLLAGFR
                     FLLASPLLGGLLTMASAVLVLYPALADNWQMSAAQIGFLYAAIPLGAAIGALTSGKLA
                     HSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYTMLQTQT
                     PEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGVLLLLVL
                     VELRRFRQTPPQVTASDS"
     gene            12966..13970
                     /gene="fepD"
                     /locus_tag="ACHUQ7_002486"
     CDS             12966..13970
                     /gene="fepD"
                     /locus_tag="ACHUQ7_002486"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFR1135851.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            13967..14959
                     /gene="fepG"
                     /locus_tag="ACHUQ7_002487"
     CDS             13967..14959
                     /gene="fepG"
                     /locus_tag="ACHUQ7_002487"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFR1135852.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAATLLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAAVLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            14956..15771
                     /gene="fepC"
                     /locus_tag="ACHUQ7_002488"
     CDS             14956..15771
                     /gene="fepC"
                     /locus_tag="ACHUQ7_002488"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFR1135853.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGMPLVVPLGRTAPSTANS"
     gene            complement(15768..16901)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHUQ7_002489"
                     /pseudo
     CDS             complement(15768..16901)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHUQ7_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
BASE COUNT         4007 a         4874 c         4453 g         3810 t
ORIGIN      
        1 ccctttcgtg aaaagccagt ggattgcgtt ctgtgggtta aaaacagcca acttatgcct
       61 aatgattaca acccaaacaa cgtggcacca cccgaaaaaa aactgctgca aaaatccatt
      121 gaaatagatg gttttaccca acccattgtg gtcacacata cggataaaaa tgccatggaa
      181 atcgtggatg gttttcaccg ccatgaaatt ggtaaaggct catcatcatt aaaattacgc
      241 ttaaaaggtt atttgccggt gacctgtctg gaaggtacgc gtaatcaacg catcgccgcg
      301 actattcgtc ataatcgcgc tcgtggacgc catcaaatca ccgccatgtc ggaaatcgtc
      361 cgtgaactta gccagttggg atgggatgat aataaaattg gcaaagagct gggaatggac
      421 agcgacgaag tgctgcgact gaagcaaatt aacggcctgc aagagttgtt tgccgaccgt
      481 caatattccc gcgcctggac ggttaaatag ctaaagctgg cgcaggcgtt cagctgctgc
      541 cagcaacgtc gattccttct tggcaaaaca gagacgaatc agtttatgtg ggaagggatc
      601 ggcgcaaaac accgacagcg gaatcgccgc tacgccgtgc tcctgcgtca gccactggca
      661 aaactcaaca tcatccaggg tagaaaccgc gctgtaatcc accagcaaaa agtatgtacc
      721 ttcacacggt aaaatctcca gccggctttc atttaaagca ttcaccagaa tatcgcgctt
      781 ctggcgataa aagtccggta acgcaagata atgctcaggt tctgcacgta gcatatcagc
      841 aagcgccagc tgtgccgggg tattcaccga aaaggtcaga tactgatgta ccttgcgaat
      901 ttcggcgctg atgggcgctg gcgcaacaca ataacccact ttccagccgg tcatatgata
      961 ggtcttgcca aatgaagaaa ccgccactgc ccgctcacgc agctgcggat gcgccagcac
     1021 actggcatgg ccctgttgtg aaaagttgat gtgctcgtag acttcatcgc taatgacaaa
     1081 aatctcgtgc ccggcgatcg cctgccacaa agcggcgaaa tcagcctgct gccagacagt
     1141 tgcactgggg ttatgcggag tgttgaggat caccagtctg gtgcgctcgc ttaataatgc
     1201 ggcaaattcc tgccagtcaa cgcgaaaatg cggtggttgc agtgccatac gtttcactat
     1261 tcccccagaa agcgcgatgg cgggggcgta actgtcatag ctgggatcaa aacaaatcac
     1321 ttcatcgcca ttgcgcacca gtgcggtaat cgccgcgtat aacgcttccg tcgcccctgc
     1381 cgttacggtg atatcgctat cggcatctgg ttgatagcca tacaaacgtt ccgttttctg
     1441 agcaatcgcc tcgcgcaagg cctgcacgcc ggtcatgggc gcgtattggt ttgccccctg
     1501 ggcaacgtgg tacgccagcc gctcctgtaa atagcgcgga ccatcaaaat caggaaagcc
     1561 ttgcgacagg ttaatcgcct ggtgttgctg cgccagcgcg ctcatctggg tgaaaatagt
     1621 ggtgccaagt tgtggaagtt tgctttgtgg aatcagaggg ttatttgtca tttttattgt
     1681 acctgacgaa gttgcgtgtt gaaggcacta taacacgatg ccagtatttg gcaatcaaga
     1741 cgtttagatg tctaaataaa acaataagga caacacaaca tgcctcacaa tcctatccgc
     1801 gtggtcgtcg gcccggctaa ctacttttca catccaggaa gtttcaatca cctgcacaat
     1861 tttttcactg atgaacaact ttctcgcgcg gtgtggatct acggcaaacg cgccattgct
     1921 gcggcgcaaa ccaaacttcc gccagcgttt ggacttccag gggcaaagca tattttgttt
     1981 cgtggtcatt gcagcgaaag cgatgtacag caactggcgg ctgagtccgg tgacgatcgc
     2041 agcgtggtga ttggcgtcgg cggcggcgca ctgctcgaca ccgcgaaagc cctcgcccgc
     2101 cgtctcggtc tgccatttgt tgccgctccg acgatcgccg ccacctgcgc agcctggaca
     2161 ccgctctccg tctggtataa cgatgccgga caggcgctgc attatgagat tttcgacgac
     2221 gccaatttta tggtgctggt ggaaccggag attatcctca atgcaccgca acaatatctg
     2281 ctggcgggga tcggtgacac gctggcgaaa tggtatgaag cggtggtgct ggctccgcaa
     2341 ccagaaacgt tgccgctaac cgtgcggctg gggatcaata atgcgcaggc cattcgcgac
     2401 gtcttgttaa acagtagcga acaggcactt gccgatcagc aaaatcagca gttaacgcaa
     2461 tcattttgcg atgtggtgga tgctattatt gctggtggtg ggatggtcgg tggtctgggc
     2521 gatcgtttta cgcgtgtggc ggcggctcat gccgtgcata acggtctgac tgtcctgccg
     2581 caaaccgaga agtttcttca cggcaccaaa gtcgcttacg gaattctggt gcaaagcgcc
     2641 ttgctgggtc aggatgatgt gctggcgcaa ttaaccgggg cgtatcagcg ttttcatctg
     2701 ccgactacgc tggcggagct ggaggtggat atcaataatc aggcggagat cgacaaagtg
     2761 attgcccaca ccttgcgccc ggtggagtcc attcattacc tgccagtcac gctgacacca
     2821 gatacgttgc gtgcagcgtt cgaaaaagtg gaatcgttta aagcctgacg tgcaaattta
     2881 gcagcagcgc gcgccgcctt ttccaccgta gcgcgcgtcc tgccgctcac ggaaaaactc
     2941 ctcgtaggtc atcggcgttt gatcgggatg attaacccgc atatgttcga catagttgtc
     3001 gtaatcaggc ataccaatca tcagcttcgc cgcctgacct aaatattttc cggcttttgc
     3061 cagtgaatca aacatcttgg cctccgctaa ccatgttgct gaaagagtgc cggatgcggc
     3121 gtgaacgctt tatccggcct acaaatgcta tcaacgcact cggtcagtcc cctcgcccca
     3181 tcggggagag ggttagggtg agggggaacc ctcggcacta tgccaacatt cgccaacatc
     3241 ccccctcact ctgactttag tgcgcgcctt tcgcctgcgc tacgatctcc tcgacatttt
     3301 ccggcatcgg ctcatacggc gtttctttcg ccgttggctt cggatctttc aatgccgcaa
     3361 gtgccgtctt aatcgagaac agtgccagga ccaccacgac caccataaag aagatggtta
     3421 acccggcatc cagacggttg ttgaacacca gttgcgccag ctgtgactca gtatactgcg
     3481 acggaatatt gccgctgtcg atcattgcct ggaacttgtt agcaatgacc aggaagccga
     3541 ctttcgcatc cgggctaaac gctttcagcc agtcagcggt cagggtacaa atcagcagcc
     3601 aggccgttgg taccagcgcc acccaggcgt aacgttgacg tttcatcttg aacaacacca
     3661 cggcacagag catcagcgcc atccctgcca gcatctggtt ggcaatacca aacagcggcc
     3721 acagagtgtt aataccgccc agcggatcga ccacgctctg atggaggaag tagccccacg
     3781 ccagtacgca cagcgctgtt gccagcaggt tagcaggcag tgaatcggtc cgcttcaggc
     3841 caggagacac cacgcccagc agatcctgca acataaagcg cgcagcacgc gtacccgcat
     3901 ctaccgccgt cagaataaac agagcttcaa acagaatggc gaagtgatac cagaacgcca
     3961 catccatcat accacccagc gcaccgtgca ggatgtaagc catccccacc gccaacgtcg
     4021 gcgcaccgcc cgcacgggaa atgatcgact gttcacccac ttcgctggca atctggttga
     4081 gcgtatctgg tgtaatgcta aagccccagc tactcaccac ctgcgcggca gaagcgacta
     4141 catccgccgt ccctgccgga gccagcaccg ccattgggct gttcatggca aaatacacgc
     4201 ccggatcgat gatacaggca gaaactagcg ccataatcgc cacgaaggat tccatcaaca
     4261 tcccaccgta gccgataaag cacgcctgcc cttcgttcgc cagcatcttc ggcgtggtgc
     4321 cggaagagat cagcgcatgg aagccagaca ccgcaccaca ggcgatggtg ataaacagga
     4381 acgggaacag gttaccggtc cataccgggc cagtgccatc gacaaatttg gtcagcgcag
     4441 gcatggtcag cgtcgggcgc ataatcaaaa tgcctaccgc cagaccgacg atagtcccga
     4501 ttttcaggaa ggtcgagaga tagtcacgcg gggccagcag caaccacacc ggcagcaccg
     4561 ccgccacaaa accgtagccc accagcatcc aggtcagctg cacgccggta aagtcaaagt
     4621 acggtgccca ggtcggactt tctgccaccc agccgccaga gataatggcg aaaatcagga
     4681 acaccaggcc aatcactgac acttcgccaa tgcggcccgg acgcagatag cgcaggtaga
     4741 ttcccataaa aatcgccagc ggaatggtga acgcaacggt atacgttccc cacgggctat
     4801 gagtcagggc tttcaccacg atcatcgcca gtactgcaag gataatgacc atgatcataa
     4861 agcaggctac cagcgcaatc accccggcag ttggccccat ctcttctttg accagctcac
     4921 ccagcgagcg accgtcacgg cgcgtagaaa caaacagcac catgaaatcc tgcaccgcac
     4981 cggcgagaac caccccggcg agcagccaga tcatccccgg caggtagccc atttgcgctg
     5041 ccagcaccgg ccccaccagc ggccctgctc cggcaatggc cgcaaaatgg tgaccgaaca
     5101 gcactttctt gtccgtcggc acatagtcca gcccgtcgtt atggcgcacc gctggcgtca
     5161 tacgcgtcgg gtcaaccgcc agcacatttt tggcgatata cagcccataa aaacggtaag
     5221 cgatcagata gatacagacc gacgccacca caatccacag cgcgttgatc tgttccccac
     5281 gatttaaagc aatgtatccc agagcaaatg ctcccattac agagagcact gtccagacga
     5341 ggtatttccc tgatttgttc atagttgtta tccgtgtgcg tgtccataga gatgttacat
     5401 tttgtttcta taacatcttt gtacgattta acaacacggt aaccatgtaa atcggagttg
     5461 aaaccaatat ttaacttaac aatgttaact cgatcactcc gttaaccgag ttttcctgca
     5521 atctcattta attctgtcgg gctcatccca aaactgccgt acccagccga caagtgcagc
     5581 aacgccgccc ctgttcatcg aaaacgacga tttcccaact ctgattttgc ctcccaagat
     5641 gcagcggctg gcagacgccg cgtaccttgc cttcagacac cgggcgatga tgggtggcgt
     5701 taagttccgt gcccaccaca cactgcccgt cacgggtcat cataaatccg gccatcgatc
     5761 ccagcgtttc agccagcgcc gccgacgcgc cgccatgcag caggccaaac ggctgatgag
     5821 tacgggtatc aaccggcatt tcggcttcca gcacatcatc tcccagaagg gtatacacaa
     5881 ttcccagatg cgccaccatt gtgttatcgc tggtggcgtt cagttcgtcg agcgttaaat
     5941 ggcgtttcca gatcatgctt atgcccccag cgttgagccg ccatcgacca caatatcctg
     6001 tagggtgata tggctggcga ggtcagaggc gaggaacaaa atcgtgttgg cgatctcttg
     6061 tggacgggcg attttcccca gcggaatgcc gagtttaaac tgctcgccaa agccgcgaat
     6121 acgctgttct tcggcgtcat cgctcaccca cagcgtgcgt tgcatatcgg tgtcggtgga
     6181 gccaggcgaa accacattac agcgcacgcc gctacccgcc agttccagcc cgacgctcaa
     6241 cgccaggctt ttcagcgccg ctttcgatgc gccataagca ctcataccaa tacgcgccgt
     6301 gtgtgcggcg tcggacgcca cagtgacaat cgccccgccc cgctgacggc gaaactggtt
     6361 catggtttgc tggaacaggt taaacgcacc gccgacgtta accgcaaaag tctgctgcca
     6421 gtcctcttta ctgagctgat cggtcgcgcc catgcgcaaa attcccgccg cattgatcag
     6481 cacgtccagt cgctccgttt cagctaacag tcgctgacat acctgcgcca cctgcccggc
     6541 gtcggcaaca tccattactt cggtcgcaaa gggatattgc tcctgagtga acgcttgatc
     6601 aaaacctgta actttcgctc ccgcctcaac aaacgtcagc gccgtggcgt agccgatacc
     6661 tttacctgcg ccggttaccc agacattttt accgctgaaa tccattattt cacctcgcgg
     6721 gagagtagct tccaccaggc gtcgatggtc gggtttttcg ccagcatgac aaagtcgata
     6781 tcaccatgca ctttgcgcca gcgcgccgcc agcgccatca tgcgcaccga atccagaccg
     6841 tagtcgatca ggttgtcgtc atcgaacggt tcatcggact cgtccagcaa cggcaggatc
     6901 acctcacgca gcgccgcttt gctggcgggg ataggtgctg gcagtaattc ttcggtcatc
     6961 accacccggc cagaacgtcc ggccacatat ttcagcgaca tcaaatgctc gtcacggctg
     7021 aaatcagcca gcgcatccgc caccataaac ggtttaatat cgcgcataaa tgcgtcggtt
     7081 gcggtggtca tacagccaat gtgggcatat actcctgtaa taatcagctg gttacgtcca
     7141 ctctctttga gcatttgctc cagcggagaa cgatgaaacg cgctgtagcg ccacttcacc
     7201 agcaccgtgt cgtcggcatc tggcgtcagg cgatccacca ccttttgctg ttccggcgag
     7261 cgggtcaggc ccggccccca catatcattc aacagcgccc gatcttcatc gctctgctct
     7321 ttcggctggg cggtgtaata aaccgggata ttgtgctgtt tgcagtagtc gcgcagcgca
     7381 gcaatattcg cgatcacctg ctccatcatc gggcagttct cgccccagaa gctgacaaaa
     7441 tagtcctgca tatcatggat taacaacgcg gcacgttgcg gttcaaaggc ccagtcaact
     7501 ttattctgcg gaatatcgtg agactccggc agtgcgtaag cctgtaattt tggaatagcc
     7561 atcgtgttct ctccttcagg ctgatgcgcg tgacgccagc cactgacgta attgtttttt
     7621 atcgactttc ccgaccgccg tcagcggaag tgaatccaca cactccacgc gatccggtaa
     7681 tttaaattcg gcaataccct gttcacgcag gaaacgacgc acctgcaccg cgcgcagcgg
     7741 ctcttttacc accagataag cacagctttt ttcgcccatc agctcatttt ccatgctcac
     7801 cagtgcggca tagatcactg ccggatggct cagcagcagg ttttcgatat cttcggcagc
     7861 gatcttctcg ccgccacggt tgatctgatc tttctcgcgc ccctgcacgg tgatgtaacc
     7921 ctctggatca atagagatca gatcgccgga acagtaaaaa ccgttggcat caaaggcact
     7981 ggcattgtgc tgcggacttt tgtaatagcc acggaaggtg tacggcccgc gcgtcatcag
     8041 gcgtccaact tccccttgcg gcagtggatt tccttcggca tcggcaaccc acacttcatc
     8101 gtccggacac attgggtaac cctgggtatg gataattttc tccgcgctat catcaagacg
     8161 ggtgtagttc accagccctt ccgccatgcc aaacacctgc tgcaactggc agccaatctc
     8221 agcgggaata cgcgctgcaa gcgtggcaga aagacgtgcg ccgccgacct gtaacagttt
     8281 cagcgaggca agctgcgccc ggctttcgcc ttcagccagc gcctgcaacc acaggctgac
     8341 tgccggcggc accagcgcgg taacgttaac ctgatgtttt gcaatcaacg ggaagcaaag
     8401 cgtggcgctg ggatcggccg ccagaacaac cgttcctccg gcaagaaaga cgcccagcga
     8461 tcctggcgaa ctcatagtgt agttatgagc cgccgggatc gagcacaggt agcgcgtctg
     8521 ttgtgtgaac tgacaaatct cggcgctacg acgcacgctg tagtaatagt cgttatgagt
     8581 gcgcgggatc agtttcggtg tgccggtggt gccgccggaa agctggaaat aggccacttc
     8641 atcagcaggt gatggcgtgg cagtaaaacc ctctgccgga tggttaatcg catcctgcaa
     8701 gttatgctca ccgctgtcgt tgtgcagttg caccacgcga atggaagaat gctctgcaac
     8761 aaatgtgttg aggaaatcat ccccgctaaa tagcgcatgc tggcgatcgg caatcagtaa
     8821 cgccggttca atctggctgg cgtaggcgtt cagttcacta cgctgatggc tgaacagcgc
     8881 cagcaccggc gcgacgccca gtttcagcag cgcgaaaaag gtaatgtaaa gttcagcgac
     8941 gttacccagt tgtaccagcg cggtttcacc aggtttaatg ccctgacggc gcaaactaca
     9001 ggcgaggtta tccgccgcct gattcagctc ccgataactc aactgccgct cgccgtcgat
     9061 aaccgcgatg ctgtcactcg cagcgtggcg agtcagaatg tcggtcagtg gcaaatcctg
     9121 ccagtagcct ttttcccgat agcgacgggc aaactcttcc ggccagcggg tgaatggaat
     9181 gctcatcctc gctccttaat gcaatccaaa aacgttcaac atggtagaaa gtttgacgcc
     9241 tgtttcgcgc cactcaccca acggtgacga cgcaggcaca atccccgctc cggcaaacag
     9301 acgcacctga ttttcccgca gcttcgcgca gcggatggtc accacccatt cgccgttacc
     9361 ttcgctgtca caccaaccca caatgccgcc aaacagttcg cggtcgaacg gctccagttc
     9421 agcaataacc tgggtcgcgg cctgatgcgg gaaaccgctc agcgcggggg tcggatgcag
     9481 cagacaggcc agagtcagtg cgttttcttg cgaattcgct ttaccttcaa agggagttgc
     9541 gagatgccac agcgtcggcg tggtaatcaa ttgtggagag gagggaacgt gtaactcact
     9601 actgcgttcg cgcagtacct ctttcatcgc ctgagtcccc agttcatgtt catggcgatc
     9661 tttttctgac gccagcagac gattacccgc ttcgcgatcg agcacttcat ccggctgacg
     9721 acgcgcggaa ccggctaacg gaatggagct aaaacgctcg ccgtctttac gtagcagcag
     9781 ttccgggctg gcccccagca ggacgccacc atcagccagc gggacatgga agttgtaact
     9841 aaccgggttt tgcgcaatca accgttccag caatacgcca ctatcaatgg cggcgtcagt
     9901 ggtgatatca atcaatcgtg acaacaccac tttgtcgacc tgcggcgtgg cggtaagtgc
     9961 ggcggcgcgg gcacccatct gttcaaacgt ggtttgttca ggaattgcct agcgttccac
    10021 cacgttcagc gactggctgc gggtaaaaca gcgggctgag gtctgttttt cctgacggga
    10081 gaacgactgc taggattcgg gaatatacag cgacgaaggc tgacgtggat cgaagggaat
    10141 cgccccgacc atcaccggat ttttgatgcc ctgcgctttg gcatcggcaa acagcgcggc
    10201 gagtttttgc tggaagggac tgtcgggcga atccccgttc acagccggtt catcgaaacg
    10261 ggcgaaacat cctgacgtcg taaaactgcg gtacggcgac ataaagaaaa agcgattggg
    10321 cgcaagtgtt gccatggtct gctgtacttc ctcagccagt gacgtatcca tatcatcctc
    10381 cacaaaatga taaaggcttt aataatgatt atcacttata ttttcggccg ctaacctaaa
    10441 agcaaacgcg cactatgtca actcttgagg taacgcaatc tgcaactcgg ggttgcatct
    10501 ggttgtggtt acaatgaaaa tgagaagcat tattgatgaa ttcgcataag cgcaatgtga
    10561 tgtcctgcgc cgttttgccc cctctcccca aagggacgga ttgtgcgctt tgtcgaattt
    10621 gtcattacgc ccttaacctt attaataaca ggaagctgac ttgtgagact cgccccgctc
    10681 taccgcaacg cccttctatt aacaggactt ttgctttcag gaatagccgt agttcaggcc
    10741 gccgactggc cgcgtcagat tactgacagc cggggcactc ataccctgga aagccagccg
    10801 cagcgtattg tttccaccag cgtcaccctg accggatcac tgctggcgat tgatgctccg
    10861 gtgatcgcca gcggcgcgac cacgccgaat aaccgcgtcg cagatgacca gggcttttta
    10921 cgccagtgga gcaaggtggc gaaagaacgc aaactgcaac ggctctatat cggcgaaccg
    10981 agcgccgaag ccgttgccgc gcaaatgccg gatctgattt taattagcgc aaccggcggg
    11041 gattcggcgc tggcgctgta tgatcagctt tccaccatcg ccccgacatt aatcatcaat
    11101 tacgacgaca aaagctggca gtcgctgtta acgcagcttg gcgaaattac cgggcatgag
    11161 aaacaagcgg cagagcggat tgcacagttt gataagcaac tggcagcggc gaaagagcaa
    11221 atcaaattac cgccgcagcc ggtcactgcc attgtctata ccgccgctgc acacagtgcc
    11281 aatctctgga cgccggaatc agcacaaggg cagatgctgg aacaactcgg ctttacgctg
    11341 gcgaaattgc ccgcagggtt aaacgccagc caaagccagg gcaaacgcca tgacatcatt
    11401 cagcttggtg gggaaaatct ggctgcgggg ttaaacggtg aatcgctatt cctgttcgct
    11461 ggtgatcaga aagatgccga tgcgatttat gccaatccac tgctcgcgca cctgcctgcg
    11521 gtacaaaaca agcaggttta tgcgctggga accgagacgt tccgcctgga ttactacagc
    11581 gccatgcaag tgctggaacg gcttaaggcg ctgttttaag cattaactgt cggacgctgt
    11641 cacctgcggc ggcgtctggc gaaaacgtcg caactccacc agcaccagca ataacaacac
    11701 gccgataatc aacaaaccaa aaccgctcgc gcttgcggaa gcaaccggtg tcatcatcgc
    11761 gcccaaacca cccagcagcg ccgcgcctat ggcatcgccc gtcacgttct gcgccgtcca
    11821 caaaccgtta atccgcccta acatcgcttc cggggtttgc gtttgcagca ttgtatattg
    11881 cagcaacgaa ctaaccgcgc tcagccagcc gaacagcgcc agacaaacca cgcctaaaat
    11941 ccacatcggc atcaggccaa acagaccaat ggcgaggaac gatcccagcg tggagagcag
    12001 catcaataac cctggtcgcg cactatgtgc cagcttcccg ctggttaacg caccaatagc
    12061 cgcgccgagc gggatcgccg cgtagagaaa accaatctgt gccgctgaca tctgccagtt
    12121 gtcagccagc gccggataca gtaccagcac cgcgctcgcc atcgtcaata aaccacccag
    12181 cagcgggctg gcaagcagaa aacgaaatcc tgccagtaat gatttcaacg gatgctcaag
    12241 cggctgcggt ggcggtggca actccggaag gcttaataac ggtaacaagg taataaacgt
    12301 gcccgccgcc gccagcccgt agttccaggc tacgccgccg gtcgccagca ataaaccgcc
    12361 aatcatgggc gaaatcaccg accccaggcg cacggtcaac atggtgatcg ccccggcctg
    12421 cattaagttt tcacgcccta ccagtgcaga tgttgccgcc agtagcgccg taacaccaag
    12481 tgatgcgaaa aaaccatccc ataaaccaag taaatagatt gccagcaatg acggctccgg
    12541 cagcagtgca ttaaggcaca gtccaatgaa gccaatgcca caggtgccgc gcgccagcaa
    12601 aatcactttt ttgcgttcat agcgatccgc cagcacaccg ccaaccatca ggccaacaaa
    12661 catcgcgccg ccggtcagcg tcaccgaaag ccccacctgc caggtagaat gggtcatcat
    12721 ctggatctgc accggcaccg cgacgccgag caaacccaga gacacaattg agataaaacg
    12781 agcgaggaat actgcgcgaa acgccgggtg cgttttcaac aggctgaggt taagcagcca
    12841 ggattgttta ttcattacaa tgccttgcca tcaaatattt taaataccta ttcttaggct
    12901 gcacatgtta acatatccaa ataagatcga taacgataat taatttcatt atcatggaag
    12961 ttcgtatatc tggttctgtt gccgtgacac gcgccattgc cgtgcccgga ttgctgttat
    13021 tactgattat cgcgacggca ttaagcctgc tcattggggc aaaatcactc cccgcttccg
    13081 tagtgctgga ggccttctcc ggcacctgcc agagcgccga ctgcaccatc gtgctcgacg
    13141 cgcggctgcc gcgtaccctt gccggtttac tggcaggcgg cgcgcttggc cttgccgggg
    13201 cgttaatgca aaccctcacc cgaaacccac ttgccgaccc cggcttgctt ggcgtgaacg
    13261 ccggagccag ctttgccatt gtgctgggtg cggcgctgtt tggttactca tccgcacagg
    13321 aacaactggc gatggccttc gccggggcgc tggtggcctc attgattgtt gcctttaccg
    13381 gcagtcaggg tggagggcag ttaagtccgg tgcgtttaac cctggcgggc gtggcgctgg
    13441 cggcggtgct ggaaggactg accagcggca tcgccctgct taatcctgac gtctacgatc
    13501 aactgcgttt ctggcaagcc ggttcgctgg atattcgcaa tctacatacc ttaaaagtgg
    13561 tgctgatccc ggttctgatc gccggagcaa ctgcgctatt actgagtcgc gcgctgaaca
    13621 gtttgagcct cggcagtgac accgcgacgg cgctgggcag tcgcgtggcg cgcacacagt
    13681 tgattggtct gctggcgatt accgtgcttt gtggtagtgc gacggcggta gttggcccga
    13741 ttgcctttat cggcctgatg atgccgcaca tggcgcgctg gctggtaggt gccgatcatc
    13801 gctggtcgct gcccgtcacg ctacttgcta cccctgccct gctgctgttt gccgatatca
    13861 tcgggcgggt gattgtgccc ggcgaactgc gcgtttctgt ggtcagtgcg tttattggtg
    13921 caccggtgct gatcttcctc gtgcgacgta aaacgcgagg tggtgcatga tttacgtctc
    13981 tcgccgatta ctcatcacct gtttgctgct gatttccgcc tgtgtggttg caggtatctg
    14041 gggattacgc agcggtgccg tcacgctgga aatctcgcag gtattcgccg cgttgatggg
    14101 cgatgcgccg cgcagtatga cgatggtggt caccgaatgg cgtttaccac gcgtgctgat
    14161 ggcgctgttg attggcgcgg cgctgggcgt cagcggcgcg atttttcagt cgctgatgcg
    14221 taacccgctc ggcagccctg acgtaatggg ctttaacacc ggggcgtgga gcggcgtgct
    14281 ggtggcgatg gtgctgtttg gtcaggatct gacggctatc gcgctggcag caatggtggg
    14341 cggcattatc acttcgctgt tggtctggct gctcgcctgg cgtaacggta tcgacacctt
    14401 tcggttgatt attatcggta tcggcgttcg cgccatgctg gtggccttta atacctggct
    14461 gttgctgaaa gcgtctttag aaacggcgct aacagcaggt ttgtggaatg ccggatcgct
    14521 caacggcctg acgtgggcaa aaacctcgcc ttccgcaccc atcattatat tgatgctcat
    14581 tgccgccacc ttgctggtgc ggcgaatgcg cttgctggag atgggcgatg ataccgcctg
    14641 cgcgctgggc gtcagcgtcg agcgttcgcg tctgttaatg atgctggttg cagtggtgct
    14701 taccgctgcg gcaacggcgc ttgccgggcc gatttccttt attgctttag tcgcgccgca
    14761 cattgcccga cgcattagcg gcaccgctcg ctgggggctg acccaggcgg cgctgtgcgg
    14821 agcgctgtta ctgctggcgg ccgttctctg cgcccaacaa ctgtttatgc cgtatcaact
    14881 tccggttggc gtcgttaccg tcagcctcgg cggtatttac cttatcgtct tgttaattca
    14941 ggagtctcgc aaaaaatgac cgaatcagta gcccgtttgc gcggcgaaca gttaaccctg
    15001 ggatatggca aatataccgt tgcggaaaat ctgactgtag aaattcctga tggtcacttc
    15061 acggcaatta tcggaccaaa tggctgtggt aaatccacgt tactgcgtac cttaagccgc
    15121 ctgatgacgc ctgctcacgg gcatgtctgg ctggatggcg agcacattca acattacgcc
    15181 agtaaagaag ttgcacgccg gattggtctg ttggcgcaaa atgctaccac gccgggcgat
    15241 atcaccgtgc aggagctggt ggcgcgtgga cgttatccgc atcaaccgct gtttacccgc
    15301 tggcgcaaag aggatgaaga agcggtaacg aaagcgatgc aggccacggg aataactcat
    15361 ctggcagatc aaagcgtgga taccctttct ggcggacaac gccagcgagc gtggatcgcg
    15421 atggtgctgg cccaggaaac ggcaattatg ctgctcgacg aacccacgac ctggctggat
    15481 atcagtcatc agattgatct actggaactg ttaagcgaac tgaaccgcga gaaaggctat
    15541 accctggcgg cggtgctgca cgatcttaat caggcctgtc gttacgccag ccatttgatt
    15601 gcattgcggg aagggaaaat tgttgctcag ggagcgccga aggagattgt cactgctgaa
    15661 ctgattgagc gcatttatgg tctgcgatgc atgatcattg acgatccggt agccggaatg
    15721 ccgcttgtgg tgccgctcgg acgaacggca ccttcaaccg caaatagtta aactaagtgg
    15781 tctgccatca tggcatcctg ttttctggat gccatcgcat agcgcaataa cacgctacca
    15841 caagccacca tcccgccgat cagcgcggaa aggatcacaa taatcgcctt acccggaccg
    15901 tcttttttca ctggcaatga cgggcttaac tgatatttaa acggcgtaaa attcacatcg
    15961 ttgacatttg cttttgttaa ttgctcgaca agatactgcc gattacgtaa ttcaccgttc
    16021 agttccgcaa cgtcagtaac cgctttttca atttccagtt tgcgttcaat accgtctgcg
    16081 ccgagggaaa tagagaaatc ggggtcatct ttaacggcct gaccattact gtatacgggc
    16141 tttttaattc ctgccgcgtt ggcaatgtcg agtgaataat tgaggcgctg aatgtttgca
    16201 tcaagttgat ttttcgtttt aatgcgatcc tgagccagtt tttctttttc aaactgggtt
    16261 ttgatctcca gtttattacg gacgttttct atcgactctt tcaccaccaa cgcagagata
    16321 taatcgatat acccgctcaa aacggtctgc gcctctttac tggttggcgc ggtaaaactt
    16381 agcgtccagg atgtatacag tgacggttca tcttttttct tactagcatt gtcatcaacc
    16441 gctttcattt tttcgctcaa tgcaacaata gcgcgatgca aatccagttc atcgattttc
    16501 gcctctttta attagtccat cacataaggt gatgaacgca ggtactcttc cagcaagcta
    16561 acagactgaa acttcttgat aaacaggtta aatgcaccgc cacggtcgat attaacatcg
    16621 agatcaagaa cgcggagttt ggtaaaggtt ttctccaggt cctgccactg aatggcttcg
    16681 gcgggagtaa taacggctga gctggtccat ttttgcggca ggatgaaaga gatcagtaaa
    16741 cctgcgcagg caaatgcaaa aacgaccgcc atgaccgttt ttttggcccg ccataaaact
    16801 tcgattagat taagtaaatc aatttcatta ttactgggcg acgccagagg atactcagaa
    16861 aaatgagcgt cacttccctg tttaatattc aatgatgaca tggggtataa cctgaaaatg
    16921 agtccaatag gggaaaatct ccacggcgct cactttacca gttggtgaga attatccgaa
    16981 gctgaagttt gtaaattcct tccactgaat gcaacattca ctctatttaa gagaaatatt
    17041 catcaaacct ataaatcaaa caaataaaaa acccataatt attttctgta attatgggta
    17101 atgactccaa cttactgata gtgttttatg ttcagataat gccc
//
LOCUS       DAZPCS010000081        17077 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZPCS010000081 DAZPCS010000000
VERSION     DAZPCS010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17077
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.081"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            71..1210
                     /gene="arnB"
                     /locus_tag="ACHUQ7_002490"
     CDS             71..1210
                     /gene="arnB"
                     /locus_tag="ACHUQ7_002490"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFR1135854.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            1214..2182
                     /gene="arnC"
                     /locus_tag="ACHUQ7_002491"
     CDS             1214..2182
                     /gene="arnC"
                     /locus_tag="ACHUQ7_002491"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFR1135855.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILINRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            2182..4163
                     /gene="arnA"
                     /locus_tag="ACHUQ7_002492"
                     /pseudo
     CDS             2182..4163
                     /gene="arnA"
                     /locus_tag="ACHUQ7_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
     gene            4160..5050
                     /gene="arnD"
                     /locus_tag="ACHUQ7_002493"
     CDS             4160..5050
                     /gene="arnD"
                     /locus_tag="ACHUQ7_002493"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFR1135856.1"
                     /translation="MTKVGLRIDVDAFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAIPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVECCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            5050..6702
                     /gene="arnT"
                     /locus_tag="ACHUQ7_002494"
     CDS             5050..6702
                     /gene="arnT"
                     /locus_tag="ACHUQ7_002494"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFR1135857.1"
                     /translation="MKSVRYLIGIFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLLAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVISPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIVRQERLVLIQYRPK"
     gene            6699..7034
                     /gene="arnE"
                     /locus_tag="ACHUQ7_002495"
     CDS             6699..7034
                     /gene="arnE"
                     /locus_tag="ACHUQ7_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFR1135858.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMMLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            7034..7420
                     /gene="arnF"
                     /locus_tag="ACHUQ7_002496"
     CDS             7034..7420
                     /gene="arnF"
                     /locus_tag="ACHUQ7_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFR1135859.1"
                     /translation="MCLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(7414..7686)
                     /gene="pmrD"
                     /locus_tag="ACHUQ7_002497"
     CDS             complement(7414..7686)
                     /gene="pmrD"
                     /locus_tag="ACHUQ7_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFR1135860.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(7791..9146)
                     /gene="menE"
                     /locus_tag="ACHUQ7_002498"
     CDS             complement(7791..9146)
                     /gene="menE"
                     /locus_tag="ACHUQ7_002498"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFR1135861.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(9143..10105)
                     /gene="menC"
                     /locus_tag="ACHUQ7_002499"
     CDS             complement(9143..10105)
                     /gene="menC"
                     /locus_tag="ACHUQ7_002499"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFR1135862.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQENWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVQEQLL"
     gene            complement(10105..10962)
                     /gene="menB"
                     /locus_tag="ACHUQ7_002500"
     CDS             complement(10105..10962)
                     /gene="menB"
                     /locus_tag="ACHUQ7_002500"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFR1135863.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(10977..11735)
                     /gene="menH"
                     /locus_tag="ACHUQ7_002501"
     CDS             complement(10977..11735)
                     /gene="menH"
                     /locus_tag="ACHUQ7_002501"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFR1135864.1"
                     /translation="MILHAQAKHGKLGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLLGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(11732..13402)
                     /gene="menD"
                     /locus_tag="ACHUQ7_002502"
     CDS             complement(11732..13402)
                     /gene="menD"
                     /locus_tag="ACHUQ7_002502"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFR1135865.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HHPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(13491..14786)
                     /gene="menF"
                     /locus_tag="ACHUQ7_002503"
     CDS             complement(13491..14786)
                     /gene="menF"
                     /locus_tag="ACHUQ7_002503"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFR1135866.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLLPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(14865..15170)
                     /gene="elaB"
                     /locus_tag="ACHUQ7_002504"
     CDS             complement(14865..15170)
                     /gene="elaB"
                     /locus_tag="ACHUQ7_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFR1135867.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKAHAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(15225..15686)
                     /locus_tag="ACHUQ7_002505"
     CDS             complement(15225..15686)
                     /locus_tag="ACHUQ7_002505"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR1135868.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            15751..16668
                     /gene="rbn"
                     /locus_tag="ACHUQ7_002506"
     CDS             15751..16668
                     /gene="rbn"
                     /locus_tag="ACHUQ7_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFR1135869.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            16859..17047
                     /locus_tag="ACHUQ7_002507"
                     /pseudo
     CDS             16859..17047
                     /locus_tag="ACHUQ7_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311184.2"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
BASE COUNT         3978 a         4623 c         4343 g         4133 t
ORIGIN      
        1 ggcaaaatat acacaacact ctttatagca aatataagtg gacaggtatt caatggcgga
       61 aggaaaagca atgtcagaat ttttgccttt ttcgcgacca gcaatgggcg tggaggaact
      121 cgctgcagtt aaagaggttc tcgaatccgg ttggatcaca accggtccga agaatcaggc
      181 gcttgagcaa gccttttgcc agttgacggg aaatcagcat gccatcgcgg tcagttcagc
      241 caccgccgga atgcatatca cgctaatggc gttggaaatt ggcaagggcg atgaagtgat
      301 tacgccttcc ctgacctggg tttcaaccct caatatgatt tccttgctgg gtgcaacgcc
      361 ggtaatggtg gatgtcgacc gcgatacgct gatggtcacg cctgaagcga tcgaatcagc
      421 cattacgcca cgcactaaag ccatcattcc ggtgcattat gccggtgcgc cagcagatat
      481 tgacgccatt cgcgccattg gcgaacgtta cggcatcgca gttatcgaag atgctgccca
      541 tgccgtcggt acgtattaca aagggcgaca tattggcgca aaaggtaccg ctattttttc
      601 atttcatgcc attaaaaata ttacctgtgc tgaaggtggc ctgattgtaa ctgataatga
      661 aaaccttgcc cgccagctac ggatgctgaa atttcacggt ctgggtgtcg atgcctatga
      721 cagacaaacc tggggccgtg caccgcaggc tgaagtctta acaccgggct ataagtacaa
      781 tctgaccgat attaacgccg cgattgccct gacacagtta gtcaaattag agcacctcaa
      841 cacccgtcgg cgcgaaattg cccagcaata tcagcaagca ctggcagctc tcccctttca
      901 gccattaagc cttcccgcct ggccgcacgt tcacgcctgg catctgttta ttattcgtgt
      961 cgatgaacaa cgttgtggta tcagtcgcga tgcgttgatg gaagcgttaa aagaaagagg
     1021 cattggtacc gggttacatt tccgcgccgc tcacacacaa aaatattatc gcgagcgttt
     1081 tcccacgctg tcgttaccga ataccgaatg gaatagcgaa cgcatctgtt cgttgccgct
     1141 gttcccggat atgactaccg ccgatgccga ccgcgtcatc acagcccttc agcaactcgc
     1201 aggacaataa gccatgtttg aaatccaccc tgttaagaaa gtctcggtgg ttattcccgt
     1261 ttataacgag caggaaagct taccggaatt aatcaggcgc accaccacag cctgtgaatc
     1321 gttggggaaa gagtatgaga tcctgctgat tgatgacggc agtagcgata attccgcgca
     1381 tatgctggtc gaagcctcac aagcggagaa cagccatatt gtgtctattt tgattaaccg
     1441 caattacggg caacattcag cgattatggc gggattcagt cacgttactg gcgacttaat
     1501 tattaccctt gatgccgatc tccagaatcc gccagaagaa atcccccgcc tggtggcaaa
     1561 agccgatgaa ggttacgacg tggtagggac tgtacgccag aaccgccagg acagctggtt
     1621 tcgtaaaaca gcttcgaaga tgattaaccg gcttattcag cgcaccactg gcaaagcgat
     1681 gggtgactac ggttgtatgc tgcgcgccta tcgccgtcat attgtcgatg cgatgttgca
     1741 ctgccatgaa cgcagcacct ttatcccgat tctggcgaat atcttcgccc gccgtgcaat
     1801 tgaaattcca gtacatcatg ccgagcgtga gtttggtgaa tccaaataca gttttatgcg
     1861 cctgattaat ttgatgtacg acctggtgac ctgccttacc acaacgccgc tacgtatgct
     1921 gagtctgctc ggcagcatta ttgcgattgg aggttttagc attgcggtgc tgctggtgat
     1981 tttacgcctg accttcggac cacaatgggc ggcagaaggc gtctttatgc tatttgccgt
     2041 gctgtttact tttattggcg ctcagtttat cggcatggga ttactcggtg aatatatcgg
     2101 caggatctac accgatgtcc gcgcccgccc ccgctatttt gttcagcaag ttatccgtcc
     2161 atccagcaag gaaaatgaat aatgaaaacc gtcgtttttg cctaccacga tatgggatgc
     2221 ctcggtattg aagctctgct ggctgccggt tacgaaatta gcgccatttt tacccatacc
     2281 gataatcccg gtgaaaaagc cttttatggt tcggtggctc atctggcggc ggaaagagac
     2341 attccggttt atgcgccgga taacgttaat catccgctgt gggtggaacg cattgcccaa
     2401 ctgtcaccag aggtgatttt ctctttttat tatcgccatc ttatttgcga cgagattttt
     2461 cagctcgctc ccgcaggtgc atttaatctg cacggttcgc tgttaccaaa atatcgtggt
     2521 cgcgcgccgc tgaactgggt gctggtgaac ggtgaaacgg aaacgggcgt tacattgcac
     2581 cgaatggtaa acgtgccgat gctggggcca ttgtggcgca actgcgcgtt gccattgcgc
     2641 cagacgatat cgctattacg ctgcatcata aattgtgcca tgccgcgcgc cagctactgg
     2701 aacagacatt acccgccatt aaacacggta atattctgga aatcgcccag cgcgaaaacg
     2761 aagccacctg ttttggtcgc agaacgccgg atgacagttt ccttgaatgg cataaaccgg
     2821 catccgtact gcacaacatg gtacgtgccg ttgccgatcc gtggccgggt gccttcagct
     2881 atgttggcaa tcagaaattc accgtctggt cgtcgcgtgt tcatcctcat gccagcaaag
     2941 cacagccggg gagcgtgatt tctgttgcgc cactgctgat tgcctgtggc gatggcgcgc
     3001 tggaaatcgt caccggacag gcgggcgacg gcattactat gcagggctcg caattagcgc
     3061 agatgctggg cctggtgcaa ggttcacgct tgaatagcca gcctgcctgc accgcccgac
     3121 gccgtacccg ggtactcatc ctcggggtga atggctttat tggcaaccat ctgacagaac
     3181 gcctgctgcg cgaagatcat tatgaagttt acggtctgga tattggcagc gatgcgataa
     3241 gccgttttct gaatcatcca cattttcact ttgtcgaagg cgatatcagt attcattccg
     3301 aatggattga gtatcacgtc aaaaaatgtg atgtcgtctt gccgctggtg gcgatagcca
     3361 cgccgattga atatacccgc aacccgctgc gcgtatttga actcgatttt gaagagaatc
     3421 tgcgcattat ccgctactgc gttaagtacc gtaagcgaat catcttcccg tcaacttcag
     3481 aagtttatgg gatgtgtagc gataaatact tcgatgagga ccattctaat ttaatcgtcg
     3541 gcccggtgaa taaaccacgc tggatttatt cggtgtcaaa acaattactt gatcgggtga
     3601 tctgggccta tggcgaaaaa gagggtttac agttcaccct cttcctcccg tttaactgga
     3661 tgggaccacg actggataac cttaatgcag cgcgaattgg cagctcccgc gctattacgc
     3721 aactcattct caatctggta gaaggttcac caattaagct aattgatggc ggaaaacaaa
     3781 aacgctgctt tactgatatc cgggacggta tcgaggcgtt gtaccacatt atcgaaaatg
     3841 cgggaaatcg ctgcgacggc gaaattatca acattggcaa tcctgagaac gaagcgagca
     3901 ttgaggaact gggcgagatg ctgctggcga gcttcgaaaa acatccgctg cgccatcatt
     3961 tcccaccgtt tgcgggcttt cgcgttgtcg aaagtagctg ctactacggc aaaggatatc
     4021 aggacgtaga gcatcgtaaa ccgagcatcc gcaatgccca ccgctgcctg gactgggagc
     4081 cgaaaattga tatgcaggaa accatcgacg aaacgctgga tttcttcctg cgcaccgttg
     4141 atcttacgga taaaccatca tgaccaaagt aggcttacgc attgatgtcg atgcctttcg
     4201 tggcacccgt gaaggcgtgc cgcgtctgct ggaaatcttg agtaagcata atattcaggc
     4261 cagcattttt ttcagcgtcg gcccggacaa tatgggccgc catctctggc gactggtgaa
     4321 gccacagttt ttgtggaaga tgctgcgctc aaacgcggca tcgctttatg gctgggatat
     4381 tttactggca ggtacggcct ggccaggtaa agagattggt catgccaatg ccgatatcat
     4441 tcgtgaagcg gctaaacatc acgaagtcgg tctgcacgcc tgggatcacc atgcctggca
     4501 agcccgtagc ggtaactggg atcggcaaac aatgatcgac gatattgcac gcggtcttcg
     4561 cactctggaa gagattatcg gtcaaccggt aacctgttct gccgctgcgg gctggcgtgc
     4621 cgaccagaag gtgatcgaag caaaagaagc gttccatttg cgctacaaca gcgattgtcg
     4681 tggggccata ccgttccgtc cactgcttga atcaggaaac cctggcactg cgcaaattcc
     4741 ggtgacctta cccacctggg atgaagtgat tggtcgggat gtgaaagcag aagattttaa
     4801 cggttggtta ctcaaccgca tcctgcgaga taaaggcacg ccggtttata ccattcatgc
     4861 agaagttgaa tgctgcgctt atcagcataa ttttgtggat ctcctcaaac gcgcagctca
     4921 ggaaggcgtg acattttgcc ctttaagcga actgttatca gagacgttgc cgctcggaca
     4981 agttgttcgc ggaaatattg ccggacgtga aggctggctg ggttgccaac aaattgcggg
     5041 tagtcgctga tgaaatcggt acgttacctt atcggcatct tcgcgtttat tgcctgctat
     5101 tacctgttac cgatcagcac gcgtctgctc tggcaacccg atgaaacgcg ttatgcggaa
     5161 atcagtcgag aaatgctggc atccggcgac tggattgtgc cccatctgtt agggctacgt
     5221 tatttcgaaa aacccattgc cggatactgg attaacagca ttgggcaatg gctatttggc
     5281 gcgaataact ttggtgtgcg ggcaggcgtt atctttgcga ccctgttaac tgccgcgctg
     5341 gtgacctggt ttactctgcg cttatggcgc gataaacgtc tggctctact cgccacagta
     5401 atttatctct cattgtttat tgtctatgcc atcggcactt atgccgtgct cgatccgttt
     5461 attgcattct ggctggtggc gggaatgtgc agcttctggc tggcaatgca ggcacagacg
     5521 tggaaaggca aaagcgcagg atttttactg ctgggaatca cctgcggcat gggggtgatg
     5581 accaaaggtt ttctcgccct tgccgtgccg gtattaagcg tgctgccatg ggtagcgacg
     5641 caaaaacgct ggaaagatct ctttatttac ggctggctgg cggttatcag ttgcgtactg
     5701 acggttctcc cctggggact ggcgatagcg cagcgggagc ctgacttctg gcactatttt
     5761 ttctgggttg agcatattca acgctttgca ctagatgatg cccaacatag agctccgttc
     5821 tggtactacg tgccggtcat cattgccggt agcctgccgt ggctgggatt actccccggt
     5881 gcactgtaca caggctggaa aaaccgcaag cattccgcaa ccgtctattt gttgagctgg
     5941 acgataatgc cgctgctgtt tttctccgtc gctaaaggca aattgcccac ctatattctt
     6001 tcttgctttg cacctctggc aatgctgttg gcgcattacg cgttgctggc agcaaaaaat
     6061 aatcctctgg cgctgcggat taatggctgg attaacatcg cttttggcgt cactggcatt
     6121 attgccacat ttgtgatctc cccctgggga ccaatgaaca cgccggtgtg gcaaaccttc
     6181 gagagctata aagtcttttg tgcctggtcg attttttcgc tatgggcatt tttcggctgg
     6241 tacaccttaa caaacgtcga aaagacctgg tctttcgccg cgctttgccc gctggggctg
     6301 gcgttgctgg taggattttc aattcctgac agagtcatgg aaggaaaaca tccgcaattt
     6361 tttgtcgaga tgacacaaga atcacttcag ccaagccgct atattcttac tgatagcgtc
     6421 ggtgttgccg caggtctggc atggagcctg caacgcgatg acatcatcat gtatcgccag
     6481 acaggtgagt tgaaatacgg ccttaattat ccggatgcga aagggagatt tgtcagcggt
     6541 gatgagttcg caaactggct taatcaacat cgtcaggagg ggattattac actcgtgctt
     6601 tcggttgacc gcgatgaaga tatcaacagt ctcgccattc cgcccgcaga tgccatcgtt
     6661 cgtcaggagc gtctggtgct gattcagtat cgtcccaaat gatctggcta acattagtct
     6721 ttgccagctt gcttagcgtt gccgggcagt tgtgtcagaa acaggcaacc tgctttgtgg
     6781 cgataaacaa acggcgcaaa catatcgtgc tgtggctggg actggcgctg gcttgtcttg
     6841 gtcttgccat gatgctctgg ctgctggtct tgcagaacgt accggtaggc attgcctacc
     6901 cgatgttaag tctgaatttt gtctgggtga cgctggctgc agtaaaactg tggcacgaac
     6961 cggtatcgcc gcgtcactgg tgtggggtgg cgttcattat tggtggcatt gtgatcctcg
     7021 ggagtacggt gtaatgtgcc tgatatgggg attattcagc gtcattattg cctcagttgc
     7081 gcaattaagc ctgggttttg cggcaagtca tctgccgccg atgacgcact tatgggattt
     7141 tatcgcggct ctgttagctt ttggcctgga tgcccggata ctgttactcg ggctgctggg
     7201 ttatctgctt tccgtttttt gttggtataa aacgctacat aaacttgcgc taagcaaagc
     7261 ctatgccttg ctcagtatga gttatgtgct ggtatggatt gcttcaatgg tacttcccgg
     7321 ctgggaaggg actttttcgc tgaaagcact actgggagta gcctgtatta tgagcgggtt
     7381 gatgctgatt ttcctgccca cgacaaaaca acgttactga gttttccctg ccgctgtaca
     7441 ctgccgttcc cactcatcgg ggctataact gcttgccgag aggactttca ccgtatgcag
     7501 cttttcacga ttaatacaat aaagcgcatt ctgcaaaggc gagagtaaat cccccacttt
     7561 cacggcgaag tcactcttca cttcggcgat catttttatc gcgccgccag catcgcagag
     7621 cataagtaca tgtctgttat cttgtttatt gcagcacgat tttttgtgcc agccattcca
     7681 ttgcattatc ctgtttgcta agagttttca catcaattta cagattaaca gataaactct
     7741 ttatcaacgg caatggaatg gcagaaatga gaagtgaaac ggagggttaa ttattgctga
     7801 cgttgcaccc actcttttag cgcctgacgt gaaattttaa taccgccgtt tttcagctcc
     7861 ggcggtaaag tcagccagcg caccggttgt tgaaaacgag ccagcttatc tttcacccat
     7921 tcgccaagat caacggtttg ctggtcatac tccaccaccg ctaccggccg atgaccaaac
     7981 tccttgtcgg caacggaaac gataaacacc tgcaaaaccg caggatgtgc agcaataaca
     8041 cgctcgactt cttccggctg aataccctca ccgccactga agaacagatt gtctaaacgt
     8101 ccgacaatgg tcagcttgcc attatgcatc tcaccgcgat cgcgcgtagc gtaccagcct
     8161 tcgtcattaa ccagtgaaac cagttgcccg ttacgccagt aaccttctgc catactggca
     8221 gcccgcagcc acacttcatt attaacgatt ttcacttccc gacccggcag cggcaaacca
     8281 acgtctgcca ggccgtcggc ttctttcgca cataccgtgg aggcaaactc ggtcagacca
     8341 tagccgcaaa agcaacgaat cccctgctcg cgcgcctgtt ccgtcaactc gaccgggata
     8401 gccgcgccgc caagtaacac cgctttcagg gaaacggaac tgcggttaac cagcaaacgc
     8461 cagagttgtg ttggcaccag tgaagcgtga gtacagcctg ccagcatttg ctccaatggc
     8521 tgtttatcac gcaccgtcat ccgcgcacca gcgtataacc agcgccacat aattccctga
     8581 ccggagacgt gaaacagcgg taaagagagc aaccagtcat cgtgatcgcc aaacggaatc
     8641 agcgataaca caccttgcgc actggcaaga tgggcctgat aagtatggac agcggctttc
     8701 ggcaaaccgg tagaacccga ggtcaacgtc attgagcaca gacgcgtcgg ctgccacgca
     8761 gcggcatgta cgccttcaac cagctgaatg tgcagcgacg ttaatgccgg aaacgtgttt
     8821 tccccatccg gcaccagcgc aaattgcagc gtcagattgg gcagcaattc ttcaagcaac
     8881 ggttgcggca gctgagggtt cacgggcaac acccgcgccc cgcattgcag taacgccagc
     8941 caggcgagca gcgtttgcgg cgtattccac gcccgcaaca tcacgccgct gccctcaacc
     9001 accccctgcg ccgcaaagcc cgtagctaac tcatcgacgc gagcacaaag ctcgcgccag
     9061 ttgagttgct cgtcattaag acgtaaggcg atggcttctc cccgcacttg ccgccagtga
     9121 cgccacggcc agtcagagaa gatcataaca actgctcctg tacatcagcg tccactaacg
     9181 gcagtgggct ccccggccag cgacgaacct gctgcgcctg catcagatcc agcgtgtcca
     9241 gtcccggaat ggtgtctggc gttaaccagg cagcaatccg cgccagttgc gttagaccta
     9301 agctcgactc aatggaagaa ctgatcaccg ccgtcagccc cagcgcatgc gccgcctgta
     9361 cctgctcgcg tactttttcc agactgcccg tgagcgtggg tttgataact accgcgcgca
     9421 cgccctcttc agccacaaag gcaaaatccg gctcgcgcag gctttcatcc caggcaatgg
     9481 caatgccggt ttcacgggca aacgctcgcg aatcatcgcg ggttttgcac ggctcttcga
     9541 gaaacgcgat gcggtggcga taatccgggt taacatattt ggcaaactgc tgacctttca
     9601 gcggtgtcca ggcgcgattt gcgtcaagac gcaaatgcag atccggaatt gcctccagca
     9661 acagattcac caccatgccg tcgcgcaccg cttcgtacaa tcccactttg accttcgcca
     9721 ctttctcgcc cggcatatcg gcaagtttga ggatcagatc gtccggatcg ccattacaca
     9781 gcggtgccgc acggtagttg gctgcttgcg gcaacgtatc tgtcagttct gccaatgcac
     9841 agcttacgcc aaaggccacg gaaggcatct gcggtagctc gcaatcgcct gccagccagt
     9901 tatttaccca ggcaagcagc acgctttgcg cctcttccca gttttcctgg ctgaagcccg
     9961 gcagtgggga gatctccccc cacccttcgc gctcgccctc acgcaggcaa acgtacagcc
    10021 cgtcgcgggt ttttaaccgc ctgtcgcgca gaaccacccc cgcgtccatg gggatctgcc
    10081 agcggtatac ctgcgcgcta cgcattacgg attccgttta aatttgctga aatcaggctg
    10141 acgtttctgg ttgaaagcgt tgcgaccttc ctgaccttct tccgtcatgt agaacagcat
    10201 ggtggcgttg cccgccagct cctgcagccc cgcctgcccg tcacagtcgg cgttcagcgc
    10261 agctttcagg cagcgcagcg ccatcgggct gttttgcagc atttcgcggc accaacggac
    10321 ggtttctttt tccagatccg ccagcggtac cacggtgttg acaaggccca tatccagcgc
    10381 ctgttttgcg tcgtactgac ggcacaggaa ccagatttct cgcgcttttt tctgcccgac
    10441 gatacgagcc atgtaggaag cgccccagcc gccgtcgaag gaaccgactt tcgggccagt
    10501 ctgaccgaag atggcattat ctgctgcgat agtcaggtcg cacatcatgt gcagaacgtg
    10561 accaccgccg atggagtagc cagccaccat cgcgacaacc ggtttcggac aggtacggat
    10621 ctgacgctgg aagtccagca cgttcaggtg atgtacgccg gaatcatctt tatagccgcc
    10681 gtaatcacca cgcactttct ggtcaccacc ggagcagaac gctttatcgc ctgcgccagt
    10741 cagaatgatc acgccgatat tgtcgtcata acgcgcatct gccagtgcct ggatcatctc
    10801 ttttaccgtc agaggacgga aggcattgcg cacctgcgga cgattaatgg tgatttttgc
    10861 gataccgtcg gtggattttt cataacgaat gtcctcgaaa ccttcggagc agtcgtgcca
    10921 ttcaaccggt gcgtaaggca ttgcttcatc aggataaatc atattgtgtc ctttagtcag
    10981 aaacgcaaga tctgcgccag acttgcgatt acgccagcgg gattttcccg atgcgcgtta
    11041 tgtccggcgc gaggaatgac atggcagtcg gcagccagtt ccgccgccag ggcgcggaat
    11101 ttgctgtcac gttcaccaca taaataataa aacgcaaatg tgcgggcgct aaggttagca
    11161 cgtaaatcag gctggacggc gagagaagtc gcctccagca tggcggcaag ggttgcgcca
    11221 ttattgttgc tgcgcagcgc caccagctcc cggcgttgat cgtcattgag cgaggcaaaa
    11281 acaggctgtt gataccagtc ggcaaatacc gccgttaacg gttctgtgcg aaaacgctgc
    11341 gcccattggc gatcggaacg ctgacgttcc gcacgttgtt cagcattttg cagccccgga
    11401 tgcccgcctt cgacaataac cccacaaagc cccgccagcc cctggcaagc cgccatcatc
    11461 gccacccgtc cgccaagcga gtaccccacc agccagaagt caaggatgtt gtaactaacc
    11521 aaggttttac gcagtaagtc ggtgacatca tcaaatccat cgacgctaat cgccgccgaa
    11581 ccaccatgac ctaggagatc aacatacaac cgtgagtagt cggcaaacgc ctcgcccact
    11641 tcttgccatt cgtggcaatc gccggaaaaa ccgtgcaaaa acaccagcca gggtaaacct
    11701 agttttccgt gttttgcctg cgcgtgcagg atcataaatg gcttacctgc gccagaagtt
    11761 gctggagcgt ttgcgcacca tcggtgtcgt taaccaccat ttcaatcacc gtggtggttg
    11821 gcgtgcgcca ggcgtcggca aatgccgttt caagttcctg ccagttttgc ggatgatgat
    11881 atttcagctc gaacatcgcg gcggcgtgct caaaatggac gttttgcggc atcagataga
    11941 aacgctcacg ctcgctttgc ggcgttggca acagcgagaa aatttgcccg ccgttgttgt
    12001 tcaccacaat taataccagc ggcgcagaaa cctgacgcaa taacgccagc gcgttgagat
    12061 cgtaaagcgc ggagagatcg cccacaatcg ccagcgtcgg tttgccgctt gcccgctgaa
    12121 cgcctgcggc ggtcgaaagc agtccgtcga taccgctggc ccctcggttg ctgtacaccg
    12181 ggtaacctgc cggaagttgc gaaagcgcat caatcagacg taccaccagg ctgttaccaa
    12241 caaacaattg cccctgttca ggcagatagt cgctgatgcg atgcgccagt tgcgcttcgc
    12301 caaacgcatc acggcgggca ataaccgcct gcattgcctg ttccgccagg cgcgggattt
    12361 caacgcacca gggctggcgt ttttctgccg gatgcagctc cagccagtcg gcaatattgg
    12421 caattaagcg acgtccgcga tggtgtgccg gatcaagtcg cccttcaatg tcatcaacaa
    12481 tccagtactc ttctggttca cagcttgcct gccattgcag gagccgtttc cccgtcaggc
    12541 tacttcccag ttgcaccaca atttgcgcct gctgcagctc gctggtcgct ttggcattgc
    12601 ctaaccaaag atcggcacac ggcagcggct gcccggtttg tgacagcaca tcgccaatca
    12661 gcggccagcc aagagtttgc gcccacaggg caactttttt gccctcttcc gcactcatgc
    12721 gcccggcaac caccacgccg cgcttttgtc gccagaagaa ccagtcgcgc tgtttttcac
    12781 tttccagacg aggcgcttca cgcagccacg gtttgtcgtc ctgccaccag tcacccagac
    12841 gctgttgcca gctaagcccg gtatcgtcca tttcgccata cagcggttca gcaaacgggc
    12901 agttgatatg gactcccccc gcatgaagcg taccgagagc gtggtcgatg gtagaaacca
    12961 gccaacgtgc ggggatatcc tgggtcgggc gcggcaatga aatactgtgc gtggggtgag
    13021 aggcgaacat tcccggctgg cgaattgcct gattcgcgcc gcagtcaatt agctccggcg
    13081 ggcgatcggc ggttaagaga atcagttttt ctccggttaa cccggcttca atcagtgccg
    13141 gatagagatt tgccaccgcc gtgccggagg tcacaatcac cgccaccggc tgcttgctga
    13201 ctttcgccag ccccagcgcc agatgcccca acccacgctc atcgaaatgg gtgtggtgaa
    13261 tgaatgcgga attctccgcc gccgctaacg ttaacggtgt agaacgcgag cctggggcga
    13321 tacagatgtg tctgacgccg tgacgcgtta atgcttccag aatgaccgcc gcccagcgtc
    13381 ggttaaatgc gcttactgac atgagtttgt ccggtatcaa tattgcggct aagtataagg
    13441 agctacaaaa atagattatt gatatgaatc ggtaatgatg cgactcatta ctattccatt
    13501 tgtaataaag tacgcagccc tgccgctttg ttgtcgattt cctgccactc ttgctcgggg
    13561 tcggaaccac ggacaatgcc cgcgccagca tataatcgca cgacattgcc gctaattttt
    13621 gctgagcgca gggaaacgca gaattcgctt tgttgtaatg agagatagcc cgcagaaccg
    13681 gcgtaccatt ctcgggtgaa cggttcgtga cgggcgataa actgtcgcgc cagatcgcgc
    13741 ggtaagccag caactgctgc cgttggcagc aactgatgta aacagatcac atcatccgct
    13801 ttgttgagtg aagtccagat acagcggcga agatgctgca ctttacgcag acgcagtacc
    13861 tgcggcggta aaacatccag cgtctgggta tcggcctgta atcgttggca gatatcttcc
    13921 accaccagca tgttctcgcg ctggttttta tcatccgcca tcagccactc tcctaactgc
    13981 tgcgcctgct tatcatcagg atgatttgct actgttcccg ccagcgcttc agtacgcagc
    14041 gctttgtcac gccgccgcca taaccgttcc ggtgaagagc caagaaaagc attttcgcca
    14101 tcaaaggcca tgtaaaaatg gtagcaattc agattcagtc gacgactggc agccatcatc
    14161 gccgccgcgt tgaccggact tgcgaaatgc aggtcagttg cccgagcgag caccactttg
    14221 tcgagctcac cttcggcgat ggttttcgtt gccagttcga ttaattgcgt ccagcccgtt
    14281 ttgtccggcc agtgttgttc tcgcgtggtg gttaaatgta acccaggcaa gggcttgata
    14341 ctcaccagtg tggcgataaa ttcttttgcc tgaatcgcat cgtgctgaag ggagctttcg
    14401 ctgaatagcg tcagccgcag cgtggcttta ccgccacagc gtcgccattc caggcggggt
    14461 aaaagtaaat tgccctgcga cgggtcaaaa gcattcagcc cccaaatgcg taagtcagcg
    14521 tgttccgggt gctggcgaag aaaacgttgt gcctggtcca acgacgtaaa acgggtaatc
    14581 gcgcccagga cggcagcttc ttcatcacca ttacgttgtt gccagtagaa ttgcgggtac
    14641 gtttgctgac tggccagcca gctcaaggca tcaaaagcgt ctttgagagg gaaaggaata
    14701 tcgataaccc gaatgccggg tgtcgccgga atctcttgcg acaaatggcg cagtagattt
    14761 tccagcgccg tagtaagtga ttgcacgcgg acctctccct gttaaaagcc tcacattata
    14821 cggggtacta caaaaaaaat gcagtacccc ggtgtaggga ggttttaacg gcgtgccagc
    14881 aacagtccta gtaccagccc gacggccgcg cccacaccaa ttccttgcca gggtttttcg
    14941 tggacgtagt catcagcacg ataaacagcc tgcttcgccc gatagtaata actgtctgaa
    15001 gcctggctaa cccgtttttt cacatcatcc agcgcttttt ctgcatgcgc tttcagctca
    15061 acatatttct gatcggcggg atcgccagag gagcggagca cctcttccag tgtttcacta
    15121 agcagcgtca ggtcgtcatc gatacgtgta tcaccaaact gattagacat tctcgttctc
    15181 catttgcgta aaacctgtca ctaactatag acaagagttg ttgattacgc ctgaattacc
    15241 tcgcgtgcca tcccaatgtg tgggatacca tcttcttcat agacctccgt cacagggata
    15301 aaaccgaaac tctggtagaa gttttgcaga tgcgcctgcg ccccgaggta aacaggctta
    15361 tcaggccagt gatgcgtaca tgtttcgagt gttttactca tcagctgctg ccccactttt
    15421 tcaccacgca acgcttcact gacaatcacc cgacctataa cgaccggctc aagatcatca
    15481 tcacttttca gaatcctcgc atacgccacc agttcatcat ttttccaacc gagaatatgg
    15541 cgattatccc ccgtcaaatc atcaccatcg atatcctgat aagggcaatt ctgctcgaca
    15601 acaaacaccg cgcaacgcag ttgtaataag gcataaagct gggaaaccga taattcagag
    15661 tgatgcagat cttgccattc aatcataatg ctctcctttt agatgtatgc cttatactag
    15721 ccggaatatt ttttgaaacg tgatgaactc atggaattaa tttttttagg tacttcagcc
    15781 ggtgtgccaa cccgcacgcg caatgtcacg gcaatattgc ttaacctgca acatccgacc
    15841 cagagcggac tttggttgtt tgactgcggt gaaggcaccc agcatcagct actgcatacc
    15901 gcctttaacc ctggaaaact ggacaagatt tttatcagtc accttcatgg cgatcatctt
    15961 tttggtttac ccggcttgct gtgcagtcgt tctatgtcag gcattatcca acccttaacg
    16021 atttatggtc cgcaaggtat ccgtgaattt gtggaaaccg cgctgcggat tagcggctca
    16081 tggaccgatt atccgctgga aattgtcgaa attggcgctg gcgaaattct cgatgatggc
    16141 ctgcgcaaag taaccgctta tccgctggaa cacccactgg aatgttatgg ctatcgtatt
    16201 gaagaacatg ataaaccggg tgcattaaat gcccaggcat taaaagctgc tggcgtgccg
    16261 cctggcccac tgtttcagga attaaaagcg ggcaaaacaa tcacgctgga agatggaagg
    16321 cagattaacg gcgcagatta cttagctgct ccagtgccag gtaaagcgct cgctattttc
    16381 ggcgataccg gcccctgcga tgccgcactt gacctggcta aaggtgtcga tgtcatggtg
    16441 cacgaagcga cgctggatat aaccatggaa gccaaagcca atagtcgcgg ccatagctct
    16501 acacgccagg ctgcgacact agcccgtgag gctggagtcg gcaagctaat cattacccac
    16561 gtcagctcgc gctatgatga caaaggttgt caacacctgt tacgtgaatg caggtcaatt
    16621 ttcccggcga ctgaactggc gaatgatttc accgtgttta acgtttaact ctgcttatta
    16681 ttaaaaacag ggtgaaactt gccctgttat agcaacccgc gctacatata tccacccaca
    16741 caggcatatt gagcgatttt tgatatttat tcatctcctt acgcactata ttcagagcaa
    16801 taagttgcct cttttcatct tgattcagca caacctttgc aactaaaagg agcccatgat
    16861 ggttacagtt gtcagcaatt attgtcaatt atctcaaacc caactcagtc agacatttgc
    16921 agaaaaattt actgtgaccg aggaattact gcagtcttta aaaaaaacag cgttatccgg
    16981 agatgaagaa agtattgagt tactgcataa tattgcgtta ggttatgatg aatttggaaa
    17041 aaaagctggg taatgctgcc aacttactga tttagtg
//
LOCUS       DAZPCS010000082        16269 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZPCS010000082 DAZPCS010000000
VERSION     DAZPCS010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16269
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.082"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(41..1036)
                     /gene="yjfF"
                     /locus_tag="ACHUQ7_002508"
                     /pseudo
     CDS             complement(41..1036)
                     /gene="yjfF"
                     /locus_tag="ACHUQ7_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
     gene            complement(1023..2045)
                     /gene="ytfT"
                     /locus_tag="ACHUQ7_002509"
     CDS             complement(1023..2045)
                     /gene="ytfT"
                     /locus_tag="ACHUQ7_002509"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFR1135870.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGITLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEVVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(2059..3561)
                     /gene="ytfR"
                     /locus_tag="ACHUQ7_002510"
     CDS             complement(2059..3561)
                     /gene="ytfR"
                     /locus_tag="ACHUQ7_002510"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFR1135871.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEMELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDELTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(3701..4657)
                     /gene="ytfQ"
                     /locus_tag="ACHUQ7_002511"
                     /pseudo
     CDS             complement(3701..4657)
                     /gene="ytfQ"
                     /locus_tag="ACHUQ7_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
     gene            4967..5497
                     /gene="ppa"
                     /locus_tag="ACHUQ7_002512"
     CDS             4967..5497
                     /gene="ppa"
                     /locus_tag="ACHUQ7_002512"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFR1135872.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVAVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5877..6218)
                     /locus_tag="ACHUQ7_002513"
     CDS             complement(5877..6218)
                     /locus_tag="ACHUQ7_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFR1135873.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(6221..10000)
                     /gene="tamB"
                     /locus_tag="ACHUQ7_002514"
     CDS             complement(6221..10000)
                     /gene="tamB"
                     /locus_tag="ACHUQ7_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFR1135874.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQLGVAVKAGNLHLGVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(9997..11730)
                     /gene="tamA"
                     /locus_tag="ACHUQ7_002515"
     CDS             complement(9997..11730)
                     /gene="tamA"
                     /locus_tag="ACHUQ7_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFR1135875.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQHVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFVGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11936..12574
                     /gene="msrA"
                     /locus_tag="ACHUQ7_002516"
     CDS             11936..12574
                     /gene="msrA"
                     /locus_tag="ACHUQ7_002516"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFR1135876.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            12897..14240
                     /locus_tag="ACHUQ7_002517"
     CDS             12897..14240
                     /locus_tag="ACHUQ7_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFR1135877.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14302..14508)
                     /locus_tag="ACHUQ7_002518"
     CDS             complement(14302..14508)
                     /locus_tag="ACHUQ7_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFR1135878.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDIGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            14833..15387
                     /locus_tag="ACHUQ7_002519"
     CDS             14833..15387
                     /locus_tag="ACHUQ7_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFR1135879.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(15450..>16269)
                     /locus_tag="ACHUQ7_002520"
     CDS             complement(15450..>16269)
                     /locus_tag="ACHUQ7_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1135880.1"
                     /translation="KDWVSNYDSLLTLRESFRCATQIDKNSYQVLSSKNETVNAMDNF
                     LISFSLKDNGAEYTITLRGSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDI
                     LQKKQSDIPKRIKGYIRQAFGDVSFGYALLEDVVSKLKRGKFELQIPGGGIKECDGWY
                     IYEKIIDDNFAIVIESLGFALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQV
                     ELAEQVDAIVSANLAADSNITKEKICEKYKSTIEAFKKEQLALPVLVRCKNSET"
BASE COUNT         3885 a         4437 c         3973 g         3974 t
ORIGIN      
        1 tccttaactg atcggcatta ctccacacgg agaggcattt ttatcgctgc gcaatgttga
       61 ctcttgtcac tggcgaactc tgacgattct cccacagcac cgttaaccca cgctgtaatg
      121 ctataaaaat aaacaacaaa ataccgatgg cgattttcgt ccactaggaa ctcagcgtgc
      181 cgtcaaagtt gatgtaagtc tggatcagtc cctgaattgc cacgccaaaa agcgtcccaa
      241 gcaccgtccc gacgccacca cttaacaacg taccgccaat caccaccgac gcgatggcgt
      301 ccagttccac acctacgccc gccagtgcat atccggcttg ggtataaatc gagaagacaa
      361 tccccgccag tgttgccagt ccggtggaga gcatataaat gcgaatagtg gtgctgcgag
      421 tagaaatccc catcaggttc gccgacgtcg cgttgccgcc aatagcgtac acctgattgc
      481 caaaacgggt acaatgcgca aggaaaatac cgataaccac caccgccagc atcaacagcc
      541 ccatcgcact aaggcgaccg ccgccgggga ttttccacgc aaggcttgag agcgtgtcat
      601 aaatgggatg gtttatcgga atcgactctt ccgaaacgag atagctgacg ccgcgcaaaa
      661 agaacatccc cgcaagcgta atgataaatg ccgggatctt cagggcgtcg atcagaagcc
      721 ccataaatgc gccgaaggca cagcccatca ccagcactag cggaaacgcc agcagcggcg
      781 agaggccgaa atcgccaacc acttttgcca gaaacacgcc agtaaaggcg atcaccgaac
      841 cgacggagag atcgataccg ccagagagga tcacaaaggt catgccaacg gcgattatcc
      901 caagaaaggc gttatcggtc aggatattgc agatcaccct tgtggaagca aaaccgggaa
      961 actgggtcag gcagtagaga tagcccagca caaagacgcc gatggtgatc atcagcggca
     1021 agttacgttt tatcatggct acgtactcct ttaatcagac tgataaagcg ttgcgactga
     1081 acaatcagca cacaaagcac caccaccgct ttcaccacct ggttcatctc cggcggaaag
     1141 cccgaaagca aaattccggt gttcatcccc tgaataatca gcgcccccac taccgagagc
     1201 agcaggttaa agcgcccgcc catcagcgat ccgccgccaa tcaccaccgc gagaatggcg
     1261 tccagctcca gccataatcc ggcgttgttg gcatcggcac cgcgaatatc cgccgccacg
     1321 ataatgcccg caatcgccgc acatagcccg ctcaacacgt aggtgagcat gacgatgatt
     1381 cgcgtgttta ccccggcatt ttttgccgcc cgaatgttga taccaacgac ttcgataaac
     1441 atccccagcg ccgttttgcg ggtcaacagc cagaacagga gaagcgtcag caccgcgata
     1501 atgaccggcg ttggcaggaa caacagcgat ccgctgccaa accatgagag atccggcgag
     1561 ttaaacgtga cgatctgccc ggcggtgatc agttgcgcca cgccgcgccc ggcgaccatc
     1621 aggatcaggg tggcgacaaa cggctgaatt ttgaggatcg ctaccagtat gccgttccac
     1681 aatcccgcca ggatgccggt gcccagggcg cttaacaaaa caatcggcag gctgaatccc
     1741 gcgaccgtca tcgcagccgt tgtggctccg gcgatagcca tcaccgcccc cacagagaga
     1801 tcgataccac cggtggcgat caccagcgta ataccaatcg ccagtaacgc aacgggagct
     1861 gcacggttaa gaatgtcgat agggctaccg aacaaacgcc catcctgaag caccacctgc
     1921 cagaaatgcg gggctaccag gctatcgacc agcagcacca gcaacagtgc cgccagctgc
     1981 ggcatccccg tcggccagtg aaaacgcctt tttggcggcg tagtgtcagg gagagattga
     2041 ggcatcacac tgttctcctt acgccgcaat ggcgttcatg atcgccggaa cggaaagctg
     2101 tgccagcggg atctccgcca cctgtttgcg atcgcgcatg atgatcaccc ggtcggcata
     2161 gcccaccagc tcttccagtt cggaggagat caccagcagc gccagaccgt cagcacacag
     2221 cgtttcaatc aggcggatga tctcggcgtg ggcaccaaca tcaatgccac gggttagctc
     2281 atcaaggatc agaaattgcg gtcgggtcag tagccaacgt gaaagcagca ctttttgctg
     2341 attgccaccg gagagaaatt caatcggttg ttcagttgaa ggtgtgcgaa tgccaagctg
     2401 gcggataaag cgttcggcaa tctcttgctg ctctttgcgg gaaatgggtc gtagccagcc
     2461 gcgctgggcc tggagagcga ggatgatatt ttcccgcacc gaggcggcgg cgatgatgcc
     2521 atcggttttc ctgtcttccg ggcagaatcc gatgcccagt actgaagcct gatgtggcga
     2581 tcgcagggtt tgcggtttgc ctttgatcaa cgccgtgccg ctgtcagcag gtttgatacc
     2641 gaagatcact tcggcggttt cggtacgtcc ggatcccagc aagccagcca gaccgacgat
     2701 ctcgccgggg cgcacttcga gatcaaacgg tgctatcgtt ccttttttgc cgtaattttt
     2761 gaacgcggca acgggtttgt cgctcaacaa tgttcgcccg gcacgctgta gcgcgtgggt
     2821 atccagctcg cgccccagca tcatttttac cagttcgatc tgcggtagct cgcgcgtttc
     2881 ccgacagcct acgaaactgc cgttgcgtaa gacggtgatc cgatcgctga cctgatagac
     2941 ctgatcgaga aagtgagtga caaagatcag gctgacgccg cgatcgcgca actgacgcat
     3001 caggccaaat aatagctcca tttcctgggt gtcgaggctg gcggtgggtt catcgaggat
     3061 cagcactttg gcggagagat cgatagcccg gcaaatggcg acgatttgct gcatcgccac
     3121 cgaaaaacgg ttaagcggtt cgcgcacgtc gagggagaaa ccgtaagatg ccatcagttc
     3181 ggtggcgcgc ttttccatct ctttacggcg tagaaggccg aaacgtttgg gttcgcgacc
     3241 tatgaataga ttatcagcaa ccgacatatt gggtagcagg ttgacttcct gatagacggt
     3301 gccgataccg agttgttgtg catgggcggt atttttcggt gagatagtct ggccttccag
     3361 ccagatggtg ccgcgatcgg cgtggtatac accagttaat gctttgatta gcgttgattt
     3421 tcccgccccg ttttcaccaa gcagcgccat gatttcgcca cggcgcaggc tgaaatcaac
     3481 gttgtctaac gctttgacgc cggggaaaaa tttacttaat ccttcggtgc ggaggatctc
     3541 ctggtgttgg tcggtcgtca tggtttcccc cggtgtaaag cgtgctccca gtaggcatga
     3601 taagacgcgg taagcgtcgc atcaggcttc agtgcaccat cgccggatgc ggcgcgagcg
     3661 ccttatccgg cctacgaatc ggcatcaggc atagcaaccc tcaatacccc atatttttct
     3721 tcttctctaa ctcttctttt gcggtatcag gcaggtaaag ggtggatttg gttaacgtca
     3781 gcttttcagg catggtgccg tcttttttgt atttctccag cgcatcgaaa gcgggtcccg
     3841 ccatgttcgg cgtcagttca acactggcgt tcgcttcgcc atccatcatc gctttgtaga
     3901 tgtccggtac accgtcaatg gaacccgtga ggatatcttt gcccggtttc aggcccgctt
     3961 ctttaattgc ctgaattgca ccaatcacca tgtcgtcgtt atgggcgtaa accatgcaga
     4021 tgtttttgcc gttgttttcc gctttgataa agctctccat gacttctttg cctttactgc
     4081 gggtgaagtc acctgactgc gagcggataa ttttgatatt tggcgcattc ttaatggctt
     4141 cggcaaagcc tttcttacgg tcaatggcga cgctggcccc aacggtgccc tgcagctcca
     4201 ccacgttgca tggtttgcca ttcacttctt ttaccagcca gtcaccgatc aacttacctt
     4261 cgaggatgtt gtcggcagta acggtggtca tatagagaga tttgtctttc acatcgatgg
     4321 aacggtcgag caagaagacc gggatttcgg catctttcgc ctcttttaat accggttccc
     4381 aacccgtcgc aaccactgga gcgataaaga tcgcatccac cccttatgca acgaaggaac
     4441 gtaccgcttt aatctggttt tcctgctttt gctgaccatc ggcaattttc aacgtaattc
     4501 cgcgtttttc ggcctcactt ttcgccacat tggtttctgc ggcgcgccag ccggattccg
     4561 atccgacctg cgaaaatcct acggttaatg gagcggccaa cgccatagac gacatggctg
     4621 ccgagactgc agagactaca agtaagcgtt tccacatagg gttgtcctcg tcggggtaat
     4681 tattattggt gaaaagatgt tcgcgaaaaa actatagaca attcgttatg taacggattg
     4741 cgttacatca cacttcaaaa aagtaaataa aacgttaatc acaagtttgt aatcgctttc
     4801 atctcactat gaaaaatgcg gctacggtta tggattttcc tgctctgtat accgtcttaa
     4861 aactggcgaa aaaggaaaat gaagacgaaa acaagcaaag acattcggcg cgagttggct
     4921 ataatacttg gcacttgttt gccacatatt tttaaaggaa acagacatga gcttactcaa
     4981 cgtccctgcg ggtaaagatc tgccggaaga catctacgtt gttattgaga tcccggctaa
     5041 cgcagatccg atcaaatacg aaatcgacaa agagagcggc gcactgttcg ttgaccgctt
     5101 catgtccacc gcgatgttct atccgtgcaa ctacggttac atcaaccaca ccctgtctct
     5161 ggacggtgac ccggttgacg tactggtccc gactccgtac ccgctgcagc ctggttctgt
     5221 gatccgttgc cgtccggttg ccgttctgaa aatgaccgac gaagccggtg aagatgcgaa
     5281 actggttgcg gttccgcaca gcaagctgag caaagaatac gatcacatta aagacgtaaa
     5341 cgatctgcct gaactgctga aagcgcaaat cgctcacttc ttcgagcact acaaagacct
     5401 cgaaaaaggc aagtgggtga aagttgaagg ttgggaaaac gcagaagccg ctaaagctga
     5461 aatcgttgct tccttcgagc gcgcaaagaa taaataagtt cttctagcgc aataaccctg
     5521 aacgccgggc ttcggttagt aagggttttt ttatgcccgc gataaataaa ctctctattc
     5581 caccatcatt attctcagcg gttgcaaggc ttgaacggta agaacaagca aacccgacca
     5641 ccattttgct gttcatagcc acttgctgga agttagccga cctcactcat actcaccgtt
     5701 caaagcgtgc ccccgtttta cccctgcaca tttgtgaacg ccagcagagt tttttatgcg
     5761 attgcagata tggaaagcgg ctcggagaga aaagcaaaag atggaaaagt tacaaaaaaa
     5821 aatcgcgaga tacatcgcat aaaacaacgc cacccgaagg aggcgtgctt ttattattac
     5881 ttatccctgt ctaaccagtc gccgctttca attagcttta atccatcgac gggtcgttga
     5941 taaacgtaca tccatgcact cccatacggc gtctgaatca actggcgcgc gtattcaccg
     6001 cccctggtgc gcaaggcatc aagttcggcc agcgtggcgt tgtcaatacg ataaacttca
     6061 ccgtgtaccg ttccgttccc cggaactgcg cctggatagt ggcccaggct atacaactgg
     6121 tagttatcga tactgaaatc gcccagtaac tgggcattgg tcatccagtg actgttgcct
     6181 tgtttgtggc gtaaactgcc gtagacaaat attcgcattg ctaaaactcg aactgataga
     6241 gcaaatccag tgcctggtct acaccagaca cggcttccag atatagctta ggcatcaggc
     6301 gataacgtaa cgtgagtgtt gctatagagt caaatatacc cacgccgtat ttcacttgca
     6361 gacctggcaa tacatagccg ctgaccacta cctgggagga gtcgcctact cccagggtgt
     6421 cgagcgctaa attgcttacg ccaaacgtct cgccgatttt acccacaatc tggccacttt
     6481 gcgcaacccc cagaccaatc agcatcgagg tcattgccgc actgtcgctc tgatcgctct
     6541 ccagcccttg tccacgtagc aaataagaca atgcagcttg ttgcgacatc gccgggtcag
     6601 agaagatctc cgctttcggt tcgtccgcca gaccagtgac gcgaactccg gcgattacat
     6661 cgtcttctgt agcatccggg ttacgaatag cttcaatatt aagatacggt tgatccggcg
     6721 gaccagagaa cagtaactca cccttacgca caatcagatc ctgaccatag gcatggaagc
     6781 gcccttcagg gatgttgatc tgcccgttca ggcccagccc ttgtttgtcc tgtacgacgt
     6841 taagatcgcc cgtcagccgc gctttcaggc caaaggcgtc aatgcgcaca ttgttgccaa
     6901 cgtggacaat caggttactg ttaatcggaa tcgacgccgt tttcggctct tccggttgca
     6961 ggttatcgtt aagcatcacc acatcgctgg agacgcctac tgcgctttcc ggcagatcgt
     7021 gcaccacgat gcgcgcccac gggacatcca cgcgaccatc gagggtaaac aggtttggtg
     7081 tagcctcgaa tacaacatct ggcgatacat ccattcgtac catcggcggc acggtgatcc
     7141 gcactttact gcctttcgcc gttactcgtg cccgccagtt ttcaatttgg ctccagtcgg
     7201 catcaccgtt caggtagatt tcaccctgct gggtccgtac tgtaccggca agcgtcgagc
     7261 gcataccgtt aaagttgacc gcaagctggc tcggctgcat atcaaacggc ataaagttgc
     7321 cgtcgatatc cacacccgta acctgaagct gaccaaacaa ctgcgggctt tgcacatcac
     7381 cacccagacg caagttggca ctcaccatcc ccgctgcttt ttccccacgg gtaaagatgg
     7441 ggtttatcat cgcaaggttg aagttacgga tattgacgtt gccaccaaga ttacggcggc
     7501 cttgcggatc ggtcacctgc acctgtccat caaactggcc gttattggtc aggcggatgg
     7561 tccagcccaa ttcggcacgg ttgttacgca attccgccgt cagattcagt gtctgaaacg
     7621 ccaccggcag cgccgcatcg ttgacggttt gcgttacctg cacgttacgc cccgaaaggg
     7681 tgatactgcc ctgcggcagc ccctctttcg tggtgtccca ggcaacatcc gctttacccg
     7741 tgaagatacc gctggcctga gtggtttctg gcataaacgg tttcagcatg gcgaggtcga
     7801 agcggttgag attcaccacc gcacgccctt cggcccccgc atcgatagtt tgcggcacgc
     7861 acagttccgc attcgggtta agccagcagt gtggcccgat gctgattttt tgctccttat
     7921 tgcggtaatc cagcgcaata tcgcgggtca gcgaccacgg gccgaccggc gtctggaagc
     7981 gggtattgct aagagttccc ttccagcgtt cttctttgcg atcaaaactt cctgccagat
     8041 taagctgccc ggagacaggc tcgccctgaa tccgcaactg tagctcgtgc tgcttttcgc
     8101 tgcctttggc attcagggtg acgaggttga tatttacatc cggctgcgaa atttgctcaa
     8161 cgcgtacgtc gagtttcccg gcgatctgat cggtggattt aatgtcgcct tccacgcgaa
     8221 cctgcgccac ggaaagttcc tgccagcgca ggccgcgcgc ggtgatatct gccagtagtt
     8281 gtggcgcttc caccgtgccg cgtactttca ccagcccttt cgctgtaccg ccaagccccg
     8341 gcagcgcgtt atccagcccc ggcgcgttga tggtggcatc aagattgaga tcttttaccc
     8401 ccagctcgcc tttcacttcg gcactgtttg gcccgagttc cagatgaagc ccagggatca
     8461 tccactgcat ataactgttg cctttcagcg tgccgtcaac gttcactttg ttctgtttga
     8521 cgttaccggt cagcttcagt tctggcacct ccatctgcca ggtgccgccg tacaggctac
     8581 cgcgggtttt aatcaagcca ttgagtttcg acggccactc cgggaactct ttggcggtgt
     8641 taatgccatt aagcgttagc tcaccgcgcc aactaatggc ctgctgccag tcgagcaacg
     8701 ccttgagttc agttttccct tccagcgccg cgacggtgag tttgtcgaga ttgacctgct
     8761 gttcattacc tttggcatca agggtaatgg tggctggcgg gatctcctgt cccttcactg
     8821 ccgtacgcat agagagcgtg taatcggtca ttttgccggt aagtttcagt ttcagatcat
     8881 ccgcctgata ctgcttctca ccagtgaacg gccagtaaag ctgtttgctg ttcacttcca
     8941 cgttgagtgg caatccggct tccgccagtc gcgtctgggc gcgtaaatcc atatccaccg
     9001 gaccggaaag gttaacgcct atctccagct gttcgcgcag cgcgccgccc actttcagct
     9061 tcaccttttc acctttcaac ggctccacgt tcagtgtgct gttgagggtg atatccaccg
     9121 gccagttgtc tgacagctgc gccgtgccgc tggcgttgac gatcccctgg ttggaatcga
     9181 tatccagggc gtccagttta gtattgccgt caatgctgct cactttcagc agcatggtgc
     9241 gcacggtgat gtccgtgtcg cccgtcacgc gcagctgctc gcccttaaac tcttcaatgt
     9301 tcaggttaag cggcaaatgc acgtcggtca tttccggcaa taccgggcga gaaaagagat
     9361 ctttcagcgt ttcgccgagc ggcttttcct ccggctgcgg attttcaatt ttcggttcga
     9421 ccacttcttc ctgcgccact tccaccactt tcggcagagc aatcagcagg cctttcagcg
     9481 acgtcggttt cagggtcagg gttttctcct gccagttcag gccggaggtg aagtccatca
     9541 ccgacaccgt ggtgtcatca atcttgatgt tgacgttgtc cagcgccacc cgtgtcaggg
     9601 tgatgggata cggcgtggag agatccagcg gaccgctatc ttcctcttct tcaacctgtt
     9661 cagaaggagg cattttttta ctgtcgatgt tgacctgaat gtctttcagc gccaggtcat
     9721 taatacaaac gctactgttc cacaggcact caagaccgac ccccagatgc agattacccg
     9781 cttttaccgc cacgcctagc tgctcataac gaacgtcaga caaggtgaga tcgcgccagc
     9841 cgccggtgac cttgccaata tccagtcctg gcacccagcg atccgccgct ttaaatacaa
     9901 gatgcaggcc gctggtggtg cccaccagaa acgccaccga tcccagcaac agtaagataa
     9961 cgataaccac gccgaggctg atttttttcc ataaactcat aattctggcc ccagaccgat
    10021 gtaaaactgt aacccgtgtt cgtctttatc cgcgaccggt acggcaaaat cgagtttgat
    10081 tggcccgacc ggcgattccc agcgtacgcc gaccccggta ccggttttaa agtcgctgcg
    10141 gcgaatatcg cttaccgctt cgccactatc gacaaacacc gcgccccacc attttccggt
    10201 cacgttgtac tggtattcca gcgatccggt tatcaacttc gaggcccctt tcaggtcacc
    10261 gttggcgtat ttcggggcga tagatttgta tttgtagccg cgaatactgc gatcgccccc
    10321 gacgaagaaa cgcagatccg gcggtacttt gtcgaaatca ccggtttcaa tccagcccag
    10381 cgtgccgcgt gtcacaaagc gatggcgatc gtacagtgtg cggatccaga cgtgctgcgc
    10441 ctggaaaacg gagaaatcga catctgaacc ccaggccgtg ttggagtagt cgatagagta
    10501 gcgttgcgag tctccccagg ttggcatcag gccaccacga gaacgcgtgc ggctaatcat
    10561 caccccagga taaaacagca tcgtggtgtt ggtaatttca ccctgggtaa agtggtcgag
    10621 gctccagcgc aggttaatgg cacgctgcca gccgctggag agatcccagt agcgagaagc
    10681 caccagcgta gtggagtcag attcggtatc gttcaggtca gtgcgcttaa aaccgccctg
    10741 caccaaataa tattgttcca gtggattctt cagcagcggc attttatagc tgaagtcgag
    10801 ggtctgttcc ggcgcggaaa tactggtact ggtggtcaaa ctgtgaccgt atgagttcat
    10861 ccacggcttt ttccacgtcg ctttcacgcg cggtcccacg tccgtagagt aaccgacccc
    10921 ggtttcgatg gtgttttctg ttcgcggcga aaccacgccc gtcaatggta atactttcgt
    10981 ttcgcgcgct ttatcaaatt gtggagccac caccaccgag ttaaaccagc cggtagcaga
    11041 aagtcggcgg ttcagttccg ccagatcttt cgattcatac tcatcgccct ctttaaacgg
    11101 caccagattt tgcaggtatt catcgcggat ttgtgatcct tcaaaggtca catgcccaaa
    11161 gcggtaacgt tcgccactgt tataatcaat atcccagaag gctttatgca ggccgagcgc
    11221 aatgcccagc tgcgctttgg taaattcgct atcgaaataa cctttacgca acgcaatact
    11281 ggttaaggac tttttgaaat tttcataatc gccctggttc agtaccgtac caatagccgg
    11341 gcgagtatcg agcaatttca aatagtcttt atcggtccgc gcgccgccgc gcaataccac
    11401 atcggtgccg ccaattaaca ccggcacacc tggcgtgact ttggcgatca atacctgccg
    11461 ccctttcttt ggcggtggac ggagatcaaa ttcaatggtc ggctggtaat aacccagcgc
    11521 tttcagacct tcacggatgg catcatcgac gcgtgcgcga aagcgacggt ctggcgtcac
    11581 ttcatcactt tcaatcgtag aaagctgcgc acgaacgttc ttttccagct gtcccgataa
    11641 cccctcgacc tgtagacgga cgttcgcggc gacggcagat ccgcttaagc agagtaagct
    11701 tacacagcat aactgtcgga tatagcgcac attttctcct gaatatcctt ttttcctgcc
    11761 cctggaacgc cgttaaacgg cttaacaaaa atccagtaat atggattaaa aagcagacta
    11821 aaccccaaat atttcttatg ttttacttta gacctattca cggtggttat tgtgtgcaaa
    11881 tacgcctctt gttacaacct taaccccaat gaccgatttt cgggagagcg acaccatgag
    11941 tttatttgat aaaaagcatc tggtttcccc cgccgatgcc ctgcctggac gtaacacccc
    12001 gatgcccgta gccacgctgc atgcggtcaa cggtcactca atgaccaatg tacctgacgg
    12061 aatggagatt gccatttttg cgatgggttg tttctggggt gtggagcgtc tgttctggca
    12121 gttacccggc gtttacagca ccgccgcagg ctataccggc ggctatacgc caaatccgac
    12181 ttatcgggaa gtgtgctccg gtgatacggg tcatgccgaa gcggtacgca ttgtttacga
    12241 tccttccgtc atcagctatg agcagttgct acaggtattt tgggagaatc acgatcccgc
    12301 ccagggaatg cgtcagggca atgaccacgg cacgcagtat cgttcagcga tttatccgct
    12361 gaccccagaa caggatgccg cagctcgcgc cagtctggaa cgttttcagg cggcgatgct
    12421 tgccgccgat gacgatcgtc acatcaccac ggaaatcgct aacgccacac cgttttatta
    12481 tgccgaagat gaccaccagc aatatctgca taaaaaccca tatggttatt gtggaattgg
    12541 cggaattggc gtctgtctgc cgccggaagc atagcgttac gggtacaaat gtagtttgtt
    12601 gataaagtgc tctttgtcta tgcctgatgc ggcttaaacg acttaccagg cctacaaaat
    12661 cgcccaaatt caatatattg caaggactgc gtaggcctga tcggcatagc gcatcaggca
    12721 gtattgcatt tatcagcagt ctgaatttta accctctggc gactttacag taccttacgc
    12781 tatactagcc actgaaaatg ccggttcact ttcttcgaat cggctttcaa tgtgtatttc
    12841 acacaaatta atcaactttc ccttccgagg gtctggcctg aaaggtcgga taagatatgt
    12901 taaacagtat tttagtcata ctctgcttga tcgctgtaag tgcgttcttc tcgatgtccg
    12961 agatctcgct tgccgcctca cgcaaaatca aacttaaact gctggctgat gaaggcaata
    13021 taaatgccca acgcgttctg aatatgcagg aaaatcccgg catgttcttt accgtggtcc
    13081 aaatcggtct gaacgcagtg gcgattctcg gcggtatcgt cggtgatgcg gcattttctc
    13141 cagcttttca cagcctgttc tcccgctata tgtcggcaga gctctctgag caactgagct
    13201 ttattctctc tttctcgtta gtgactggca tgtttatcct gtttgcggat ttaaccccga
    13261 aacgcatcgg tatgattgcg ccagaagcgg tggctttgcg tatcatcaac ccgatgcgct
    13321 tctgcctgta cgtttgcacc ccgctggtgt ggttcttcaa cggcctggcg aacatgatct
    13381 tccgtatttt caaactgcca atggtacgta aagatgacat cacttctgat gacatctacg
    13441 cggtagtgga agccggtgcg ctggcgggcg tgttacgtaa acaggaacac gagctgattg
    13501 aaaacgtctt tgagctggaa tcccgtaccg ttccgtcttc aatgacaccg cgtgaaaacg
    13561 tgatttggtt tgatctccac gaagatgagc aaagcctgaa gaataaggtg gcggaacatc
    13621 cgcactctaa gttcctcgtc tgtaatgaag atattgacca catcatcggt tatgtcgatt
    13681 ctaaagacct gctgaaccgc gtgctggcta accaaagcct ggcactgaac agcggcgtac
    13741 aaattcgcaa cacgctgatt gtgccggata cgttaaccct ttcagaggcg ttggaaagtt
    13801 ttaaaaccgc aggtgaagac ttcgcggtga tcatgaacga gtacgcgctg gtggtgggga
    13861 tcatcaccct caacgacgtg atgaccacgc tgatgggcga tctggtcggt caggggctgg
    13921 aagagcagat tgtcgcccgt gatgagaact catggctgat tgacggcggc accccaattg
    13981 acgacgtcat gcgcgtgctg gatattgacg agttcccgca gtcgggcaac tacgaaacca
    14041 tcggcggctt tatgatgttt atgctgcgta agatcccgaa acgcaccgat tcggtgaaat
    14101 tcgccggcta caaatttgaa gtggtggata tcgataacta ccgcatcgac cagctgctgg
    14161 tgacccggat cgacagcaag gccaccgccc tttcgccaaa actgcctgac gctaaagata
    14221 aagaagaaag cgtcgcgtaa cccccagaaa catcaacggc tcctgaatca ggagccgtct
    14281 tattactgca tagcactttg gttaagccat ctctgtttgc agacgcataa cctgacggtt
    14341 aacttcggac atcactgaca aatgcagctt atccttcact tttgggataa gaatcttacc
    14401 cttatcaaat tcaaaagcgc caatgtcctt gatgtataac cgtccacgga acaggatctt
    14461 tacgtacttc gccacctgaa gtgggttgta ccgttggaaa attttcattg ttatctcctg
    14521 ctgagtatta cgcctgtgcg gtgccacaat cggcccaact attatgaggc gcaaatttta
    14581 atgcttagtg actatagact atccgggcaa tgtttccacc gcgtataact ttttttacct
    14641 aaaggttaca attattcaga attatctttt taccgaagcg cggtcttcag tataagcatt
    14701 catttttcat atgatttgtg cgcttgaccg caaactggca tcacacttgc gggaaattcg
    14761 ataaatagca catatgatta aaactcagac ccaagtggtc ggatcacctg catatcataa
    14821 gaaggaaaca ccatgaccct acgcaagatt ctggcactca cctgcctgct gttgccgatg
    14881 atggcttccg cacatcagtt cgaaatcggt cagcgagtgc cgccgattgg cattaccgat
    14941 cggggcgagt tggtgcttga taaagatcag tttagctaca aaacctggaa cagcgcgcag
    15001 ttagtgggaa aagtgcgagt actgcaacat attgctggtc gcacctctgc aaaagagaaa
    15061 aacgcgacgc tgattgaagc gattaaatca gcgaagttac cgcacgatcg ttaccagacc
    15121 accaccattg ttaacaccga cgacgcgatt ccgggttccg gcatgtttgt gcgcagcagt
    15181 ctggagagta ataaaaagct ttatccctgg tcgcagttta ttgtcgatag caatggcgtc
    15241 gcgcgcggtg cctggcagct ggatgaagaa agttccgctg tagtggtgct ggataaagac
    15301 ggtcgcgtgc aatgggccaa agacggggcg ctcacaccgg aagaggtgca gcaagtgatg
    15361 gacctgctgc aaaagttgct caaataatgg caataacata aattctggaa aaataatttt
    15421 cccctatcgt tatctatggg ataggggaat taagtctctg agttcttaca ccgaactaat
    15481 actggtaacg ctagttgctc ttttttaaac gcctcaatgg tcgatttata tttctcgcaa
    15541 atcttttctt tggtaatatt actatctgcg gccagattcg cacttactat cgcatccact
    15601 tgttcggcta attcgacttg ttggcaacct gcattaacca gaaaattata aataagagag
    15661 taatcttcat cctccaaaac aacagaggaa ccattacgaa aacgttcatc ccctccatag
    15721 attttaagcg caaagcccaa agattctatg actatggcaa aattatcatc aattattttt
    15781 tcgtaaatat accatccatc gcattcttta attccaccgc ctggtatttg gagttcaaat
    15841 ttaccccgtt ttaatttgct tactacatcc tccaataagg cataaccgaa agatacgtca
    15901 ccaaacgcct ggcgaatata acctttaatc ctttttggta tgtcagactg ttttttctgc
    15961 aaaatatcac aggcatacaa tcttctgttt tgttctaacg gaaactcgcg gtttttgaaa
    16021 tccatgaatg agttatactc atttattgtt atgggaattt cttcgtattc aaacccactg
    16081 cctcgcaatg ttattgtata ttcagcaccg ttatctttaa ggctgaaact gataagaaaa
    16141 ttatccatgg cattaacagt ttcatttttt gatgaaagca cttgataact atttttatca
    16201 atctgtgttg cgcaacgaaa ggactcccgc aaagttaaca gactatcata attcgaaacc
    16261 caatctttt
//
LOCUS       DAZPCS010000083        16096 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZPCS010000083 DAZPCS010000000
VERSION     DAZPCS010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16096
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.083"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..588
                     /locus_tag="ACHUQ7_002521"
     CDS             <1..588
                     /locus_tag="ACHUQ7_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HFR1135881.1"
                     /translation="IPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEV
                     EIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKP
                     HTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKM
                     VVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLG"
     gene            771..965
                     /gene="bfd"
                     /locus_tag="ACHUQ7_002522"
     CDS             771..965
                     /gene="bfd"
                     /locus_tag="ACHUQ7_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFR1135882.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            1038..1514
                     /gene="bfr"
                     /locus_tag="ACHUQ7_002523"
     CDS             1038..1514
                     /gene="bfr"
                     /locus_tag="ACHUQ7_002523"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFR1135883.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(1511..1978)
                     /locus_tag="ACHUQ7_002524"
                     /pseudo
     CDS             complement(1511..1978)
                     /locus_tag="ACHUQ7_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
     gene            2357..2668
                     /gene="rpsJ"
                     /locus_tag="ACHUQ7_002525"
     CDS             2357..2668
                     /gene="rpsJ"
                     /locus_tag="ACHUQ7_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFR1135884.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2701..3330
                     /gene="rplC"
                     /locus_tag="ACHUQ7_002526"
     CDS             2701..3330
                     /gene="rplC"
                     /locus_tag="ACHUQ7_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFR1135885.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            3341..3946
                     /gene="rplD"
                     /locus_tag="ACHUQ7_002527"
     CDS             3341..3946
                     /gene="rplD"
                     /locus_tag="ACHUQ7_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFR1135886.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLQKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3943..4245
                     /gene="rplW"
                     /locus_tag="ACHUQ7_002528"
     CDS             3943..4245
                     /gene="rplW"
                     /locus_tag="ACHUQ7_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFR1135887.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            4263..5084
                     /gene="rplB"
                     /locus_tag="ACHUQ7_002529"
     CDS             4263..5084
                     /gene="rplB"
                     /locus_tag="ACHUQ7_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFR1135888.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            5101..5379
                     /gene="rpsS"
                     /locus_tag="ACHUQ7_002530"
     CDS             5101..5379
                     /gene="rpsS"
                     /locus_tag="ACHUQ7_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFR1135889.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            5394..5726
                     /gene="rplV"
                     /locus_tag="ACHUQ7_002531"
     CDS             5394..5726
                     /gene="rplV"
                     /locus_tag="ACHUQ7_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFR1135890.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VQVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5744..6445
                     /gene="rpsC"
                     /locus_tag="ACHUQ7_002532"
     CDS             5744..6445
                     /gene="rpsC"
                     /locus_tag="ACHUQ7_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFR1135891.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            6458..6868
                     /gene="rplP"
                     /locus_tag="ACHUQ7_002533"
     CDS             6458..6868
                     /gene="rplP"
                     /locus_tag="ACHUQ7_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFR1135892.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6868..7059
                     /gene="rpmC"
                     /locus_tag="ACHUQ7_002534"
     CDS             6868..7059
                     /gene="rpmC"
                     /locus_tag="ACHUQ7_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFR1135893.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            7059..7313
                     /gene="rpsQ"
                     /locus_tag="ACHUQ7_002535"
     CDS             7059..7313
                     /gene="rpsQ"
                     /locus_tag="ACHUQ7_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFR1135894.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7478..7849
                     /gene="rplN"
                     /locus_tag="ACHUQ7_002536"
     CDS             7478..7849
                     /gene="rplN"
                     /locus_tag="ACHUQ7_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFR1135895.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7860..8174
                     /gene="rplX"
                     /locus_tag="ACHUQ7_002537"
     CDS             7860..8174
                     /gene="rplX"
                     /locus_tag="ACHUQ7_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFR1135896.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIIEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            8189..8728
                     /gene="rplE"
                     /locus_tag="ACHUQ7_002538"
     CDS             8189..8728
                     /gene="rplE"
                     /locus_tag="ACHUQ7_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFR1135897.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8743..9048
                     /gene="rpsN"
                     /locus_tag="ACHUQ7_002539"
     CDS             8743..9048
                     /gene="rpsN"
                     /locus_tag="ACHUQ7_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFR1135898.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            9082..9474
                     /gene="rpsH"
                     /locus_tag="ACHUQ7_002540"
     CDS             9082..9474
                     /gene="rpsH"
                     /locus_tag="ACHUQ7_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFR1135899.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9487..10020
                     /gene="rplF"
                     /locus_tag="ACHUQ7_002541"
     CDS             9487..10020
                     /gene="rplF"
                     /locus_tag="ACHUQ7_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFR1135900.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            10030..10383
                     /gene="rplR"
                     /locus_tag="ACHUQ7_002542"
     CDS             10030..10383
                     /gene="rplR"
                     /locus_tag="ACHUQ7_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFR1135901.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            10398..10901
                     /gene="rpsE"
                     /locus_tag="ACHUQ7_002543"
     CDS             10398..10901
                     /gene="rpsE"
                     /locus_tag="ACHUQ7_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFR1135902.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10905..11084
                     /gene="rpmD"
                     /locus_tag="ACHUQ7_002544"
     CDS             10905..11084
                     /gene="rpmD"
                     /locus_tag="ACHUQ7_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFR1135903.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            11088..11522
                     /gene="rplO"
                     /locus_tag="ACHUQ7_002545"
     CDS             11088..11522
                     /gene="rplO"
                     /locus_tag="ACHUQ7_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFR1135904.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11530..12861
                     /gene="secY"
                     /locus_tag="ACHUQ7_002546"
     CDS             11530..12861
                     /gene="secY"
                     /locus_tag="ACHUQ7_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFR1135905.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12893..13009
                     /gene="rpmJ"
                     /locus_tag="ACHUQ7_002547"
     CDS             12893..13009
                     /gene="rpmJ"
                     /locus_tag="ACHUQ7_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFR1135906.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            13156..13512
                     /gene="rpsM"
                     /locus_tag="ACHUQ7_002548"
     CDS             13156..13512
                     /gene="rpsM"
                     /locus_tag="ACHUQ7_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFR1135907.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13529..13918
                     /gene="rpsK"
                     /locus_tag="ACHUQ7_002549"
     CDS             13529..13918
                     /gene="rpsK"
                     /locus_tag="ACHUQ7_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFR1135908.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13952..14572
                     /gene="rpsD"
                     /locus_tag="ACHUQ7_002550"
     CDS             13952..14572
                     /gene="rpsD"
                     /locus_tag="ACHUQ7_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFR1135909.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14598..15587
                     /gene="rpoA"
                     /locus_tag="ACHUQ7_002551"
     CDS             14598..15587
                     /gene="rpoA"
                     /locus_tag="ACHUQ7_002551"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFR1135910.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15628..16011
                     /gene="rplQ"
                     /locus_tag="ACHUQ7_002552"
     CDS             15628..16011
                     /gene="rplQ"
                     /locus_tag="ACHUQ7_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFR1135911.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
BASE COUNT         4141 a         3783 c         4292 g         3880 t
ORIGIN      
        1 attccggaac cagagcgtgc gattgacaag ccgttcctgc tgccgatcga agacgtattc
       61 tccatctccg gtcgtggtac cgttgttacc ggtcgtgtag aacgcggtat catcaaagtt
      121 ggtgaagaag ttgaaatcgt tggtatcaaa gagactcaga agtctacctg tactggcgtt
      181 gaaatgttcc gcaaactgct ggacgaaggc cgtgctggtg agaacgtagg tgttctgctg
      241 cgtggtatca aacgtgaaga aatcgaacgt ggtcaggtac tggctaagcc gggcaccatc
      301 aagccgcaca ccaagttcga atctgaagtg tacattctgt ccaaagatga aggcggccgt
      361 catactccgt tcttcaaagg ctaccgtccg cagttctact tccgtactac tgacgtgact
      421 ggtaccatcg aactgccgga aggcgtagag atggtaatgc cgggcgacaa catcaaaatg
      481 gttgttaccc tgatccaccc gatcgcgatg gacgacggtc tgcgtttcgc aatccgtgaa
      541 ggcggccgta ccgttggcgc gggcgttgtt gctaaagttc tgggctaatt acacgttaat
      601 tagttttgaa ttgaaaaggg cgcttcggcg ccctttttgc tgcccgtaat ttgagctcga
      661 aatatttagt cgtaattttg ctcgctgaac tatggcgcaa ataaaactgt gctattgtaa
      721 tcataaccat tctcatttac actttgtgca gaatttgaac gggagcaatc atgtacgttt
      781 gtttgtgtaa tggtgtaagt gacaaaaaaa ttcgccaggc agtccgtcag tttcatccgc
      841 aatctttcca acaattgcgt aagtttattc ctgtgggaaa tcaatgcggt aagtgcattc
      901 gcgccgcgcg cgaagtgatg caggacgagt tgatgcagat gccagaattt aaagagattg
      961 cctgaagccc acgcttattt tttgacatcc tgctagcccc atctacgctc taatagtgga
     1021 agcggaggga ctataaaatg aaaggtgatg ttaaaatcat aaattatctc aataaactat
     1081 tgggaaatga gcttgtcgca atcaatcagt attttctcca tgcccggatg tttaaaaact
     1141 ggggcctgat gcgtctcaat gacgtcgaat accatgaatc catcgatgag atgaaacacg
     1201 ctgataaata tatcgagcgt attttgtttt tagaaggaat ccccaactta caggatttag
     1261 gcaaactggg gattggtgaa gatgtcgagg agatgctgca atctgacctg cgattagaac
     1321 tggaaggcgc gaaggatctg cgtgaggcta tcgcatatgc cgacagcgtt catgactatg
     1381 ttagccgcga tatgatgatc gagatccttg ctgatgaaga aggtcatatc gactggctcg
     1441 aaactgaact ggatctcatc ggtaaaatcg gcctacaaaa ttacctgcaa tcacaaatta
     1501 aagttaaaga ttaacggcct atcagcaaac tatcccagcc tataacgaaa cccgcagccg
     1561 ccagaaatgg cccaaaaggc agcgggtttt ttaatgattc ttttcctctc atgagcagac
     1621 caataactac ggctccgcaa gcgaatgagg cagcaaggaa aaccagtcgt ggtaagaagg
     1681 cccatgaatg ctaggcgcca agggcggcga gaaacttcac atcaccatag cctaatcctt
     1741 ctttatgtcg cagtatgcga tacccccagt aaatgacagc gaatgtgccg taaccaacaa
     1801 ttgcccccca taacgcatcg gctaaaccat caggatgaca aacttgatag aacaaaagac
     1861 ctgaccagag taacgggcac gtaaatctgt caggtaacaa gccatgcttt gcatcccaga
     1921 aaaaaagtag ggctgataaa caagcgtaca aaattagaaa gggaagggta gtgtccataa
     1981 aacatcctct gtaattggag tatcaccatc gaataactcc ggatgctcag caacagagag
     2041 agcgctgatt tgttaacctg attccggtga aaaacgctat cttcacaacc ttacaaattt
     2101 cgtggaatgc agctcgcaat ctgcgcgttg cgattaattt ctacccgatg cttgcgtcag
     2161 gctcagattt gcgtataatg cgcgggcttg tcgtagttga cagcaggttc aatctgaacc
     2221 tcagttagcc gaatttggct acctaacaat gctcccaatc ggggagctac gtaagaacgg
     2281 ttacactctc ccatcaatcg taatgggtct gaggagtaat cattttcgtt tataaaataa
     2341 ttggagctct ggtctcatgc agaaccaaag aatccgtatc cgcctgaaag cgtttgatca
     2401 tcgtctgatc gatcaagcaa ccgcggaaat cgtcgagact gccaagcgca ctggtgcgca
     2461 ggtccgtggt ccgatcccgc tgccgacacg caaagagcgc ttcactgttc tgatctcccc
     2521 gcacgtcaac aaagacgcgc gcgatcagta cgaaatccgt actcacttgc gtctggttga
     2581 catcgttgag ccaaccgaga aaaccgttga tgctctgatg cgtctggatc tggctgccgg
     2641 tgtagacgtg cagatcagcc tgggttaatc aggtcattga gcgattgaga ggttgaaaca
     2701 atgattggtt tagtcggtaa aaaagtgggt atgacccgta tcttcacaga agacggcgtt
     2761 tctatcccag taaccgtaat cgaagttgaa gcaaaccgcg ttactcaggt taaagacctg
     2821 gctaacgatg gctaccgtgc tattcaggtg accaccggtg ctaaaaaagc taaccgtgtg
     2881 accaagcctg aagctggcca cttcgctaaa gctggcgtag aagctggccg tggtctgtgg
     2941 gaattccgcc tggctgaagg cgaagagttc actgtaggtc agagcattag cgttgaactg
     3001 tttgctgacg ttaaaaaagt tgacgtaact ggcacctcta aaggtaaagg tttcgcaggt
     3061 accgttaagc gctggaactt ccgtacccag gacgctactc acggtaactc cttgtctcac
     3121 cgcgttccgg gttctatcgg tcagaaccag actccgggca aagtgttcaa aggcaagaaa
     3181 atggcaggtc agatgggtaa cgaacgtgta accgttcaga gccttgacgt agtacgcgtt
     3241 gacgctgagc gcaacctgct gctggttaaa ggtgctgtcc cgggtgcaac cggtagcgac
     3301 ctgatcgtta aaccagctgt gaaggcgtaa ggagatagca atggaattag tattgaaaga
     3361 cgcgcagagc gcgctgactg tttccgaaac taccttcggt cgtgatttca acgaagcgct
     3421 ggttcaccag gttgttgttg cttatgcagc tggtgctcgt cagggtactc gtgctcagaa
     3481 gactcgtgct gaagtaactg gttccggtaa aaaaccgtgg cgccagaaag gcaccggccg
     3541 tgcgcgttct ggttctatca agagcccgat ctggcgttct ggtggcgtga cctttgctgc
     3601 tcgtccgcag gaccacagtc aaaaagttaa caagaagatg taccgcggcg cgctgaaaag
     3661 catcctgtcc gaactggtac gtcaggatcg tctgatcgtt gtcgagaagt tctctgtaga
     3721 agcgccgaaa actaagctgc tggcacagaa actgaaagac atggctctgg aagatgtgct
     3781 gatcatcacc ggtgagctgg acgaaaacct gttcctggct gcgcgcaacc tgcagaaggt
     3841 tgacgtacgc gatgcaactg gtatcgaccc ggttagcctg atcgccttcg acaaagtcgt
     3901 aatgactgct gatgctgtta agcaagttga ggagatgctg gcatgattcg tgaagaacgt
     3961 ctgctgaagg tgctgcgtgc accgcacgtt tctgaaaaag cgtctactgc gatggaaaaa
     4021 tccaacacca tcgtactcaa agttgctaaa gacgcgacca aagcagaaat caaagctgct
     4081 gtgcagaaac tgtttgaagt cgaagtcgaa gtcgttaaca ccctggtagt taaagggaaa
     4141 gttaaacgtc acggacagcg tatcggtcgt cgtagcgact ggaaaaaagc ttacgtcacc
     4201 ctgaaagaag gccagaatct ggacttcgtt ggcggcgctg agtaagtcgg aggagtaata
     4261 caatggcagt tgttaaatgt aaaccgacat ctccgggtcg tcgccacgta gttaaagtgg
     4321 ttaaccctga gctgcacaag ggcaaacctt ttgctccgtt gctggaaaaa aacagcaaat
     4381 ccggtggtcg taacaacaat ggccgtatca ccactcgtca tatcggtggt ggccacaagc
     4441 aggcttaccg tattgttgac ttcaaacgca acaaagacgg tatcccggca gttgttgaac
     4501 gtcttgagta cgatccgaac cgttccgcga acatcgcgct ggttctgtac aaagacggtg
     4561 aacgccgtta catcctggcc cctaaaggcc tgaaagctgg cgaccagatt cagtctggcg
     4621 ttgatgctgc aatcaaacca ggtaacaccc tgccgatgcg caacatcccg gttggttcta
     4681 ctgttcataa cgtagaaatg aaaccaggta aaggcggtca gctggcacgt tccgctggta
     4741 cttacgttca gatcgttgct cgtgatggtg cttatgtcac cctgcgtctg cgttctggtg
     4801 aaatgcgtaa agtagaagca gactgccgtg caactctggg cgaagttggc aatgctgagc
     4861 atatgctgcg cgttctgggt aaagcaggtg ctgcacgctg gcgtggtgtt cgtccgaccg
     4921 ttcgcggtac cgcgatgaac ccggtagacc acccacatgg tggtggtgaa ggtcgtaact
     4981 ttggtaagca cccggtaact ccgtggggcg ttcagaccaa aggtaagaag acccgcagca
     5041 acaagcgtac tgataaattc atcgtacgtc gccgtagcaa ataattttag aggataagcc
     5101 atgccacgtt ctctcaagaa aggtcctttt attgacctgc acttgctgaa gaaggtagag
     5161 aaagcggtgg aaagcggaga caagaagccc ctgcgcactt ggtcccgtcg ttcaacgatc
     5221 tttcctaaca tgatcggttt gaccatcgct gtccataatg gtcgtcagca cgttccggta
     5281 tttgtaaccg acgaaatggt cggtcacaaa ctgggtgaat tcgcaccgac tcgtacttat
     5341 cgcggccacg ctgctgataa aaaagcgaag aagaaataag gtaggaggaa gagatggaaa
     5401 ctattgctaa acatcgccat gctcgttctt ctgctcagaa ggttcgcctt gttgctgacc
     5461 tgattcgcgg taagaaagtg tcgcaggctc tggatatttt gacctacacc aacaagaaag
     5521 cggctgtaca ggtcaagaaa gttctggaat ctgccattgc taacgctgaa cacaacgatg
     5581 gcgctgacat tgacgatctg aaagttacga aaattttcgt agacgaaggc ccgagcatga
     5641 agcgcattat gccgcgtgca aaaggtcgtg cagatcgcat cctgaagcgc accagccaca
     5701 tcactgtggt tgtgtccgat cgctgagact ctggagacta gcaatgggtc agaaagtaca
     5761 tcctaatggt attcgcttgg gtattgtaaa accatggaac tctacttggt ttgcgaacac
     5821 caaagaattc gctgacaacc tggacagcga ttttaaagta cgtcagtacc tgactaagga
     5881 actggctaaa gcgtccgtat ctcgtatcgt tatcgagcgt ccggctaaga gcatccgtgt
     5941 aaccattcac actgctcgcc cgggtatcgt tatcggtaaa aaaggtgaag acgtagaaaa
     6001 actgcgtaag gtcgtagcgg acatcgctgg cgttcctgca cagatcaaca tcgccgaagt
     6061 tcgtaagcct gaactggacg caaaactggt tgctgacagc atcacttctc agctggaacg
     6121 tcgcgttatg ttccgtcgtg ctatgaagcg tgctgtacag aacgcaatgc gtctgggcgc
     6181 taaaggtatt aaagttgaag ttagcggccg tctgggcggc gcggaaatcg cacgtaccga
     6241 atggtaccgc gaaggtcgcg taccgctgca cactctgcgt gctgacatcg actacaacac
     6301 ctctgaagcg cacaccactt acggtgtaat cggcgttaaa gtgtggatct tcaaaggcga
     6361 gatcctgggt ggtatggctg ctgttgaaca accggaaaaa ccggctgctc agcctaaaaa
     6421 gcagcagcgt aaaggccgta aataaggagc gtcgctgatg ttacaaccaa agcgtacaaa
     6481 attccgtaaa atgcacaaag gccgtaaccg cggtctggcg cagggtacgg atgttagctt
     6541 cggcagcttc ggtctgaaag ctgttggccg tggtcgtctg actgcccgtc agatcgaagc
     6601 agcacgtcgt gctatgaccc gtgcagttaa gcgtcaaggt aagatctgga tccgtgtgtt
     6661 cccggacaaa ccgatcactg aaaagccgct ggcagtgcgt atgggtaaag gtaaaggtaa
     6721 cgtggagtat tgggttgcct tgattcagcc gggtaaagtc ctgtatgaaa tggacggtgt
     6781 tccggaagag ctggcccgtg aagcattcaa gctggcagca gcgaaactgc cgattaaaac
     6841 cacctttgta actaagacgg tgatgtaatg aaagcaaaag agctgcgtga gaagagcatt
     6901 gaagagctga acaccgagct gctgaacctg ctgcgtgagc agttcaacct gcgtatgcag
     6961 gctgcaagtg gccagctgca acagtctcac ctgttgaagc aagtgcgtcg cgatgtcgca
     7021 cgcgttaaga ctttactgaa cgagaaggcg ggtgcgtaat gaccgataaa atccgtactc
     7081 tgcaaggtcg cgttgttagc gacaaaatgg agaaatccat tgttgttgct atcgaacgtt
     7141 ttgtgaaaca cccgatctac ggaaaattca tcaagcgtac gaccaaactg cacgtacatg
     7201 acgagaacaa cgaatgcggt atcggtgacg tggttgaaat ccgcgaatgc cgtccgctgt
     7261 ccaagactaa atcctggacg ctggttcgcg ttgtagagaa agcggttctg taatacagta
     7321 cactctctcg atacgaataa acggctcaga aatgagccgt ttattttttc tacccatatc
     7381 cttgaagcgg tgttataatg ccgcgccctc gatatgggga tttttaacga cctgattttc
     7441 gggtctcagt agtagttgac attagcggag cactaaaatg atccaagaac agactatgct
     7501 gaacgtcgcc gacaactccg gtgcacgtcg cgtaatgtgt atcaaggttc tgggtggctc
     7561 gcaccgtcgc tacgcaggcg taggcgacat catcaagatc accatcaaag aagcaattcc
     7621 gcgtggtaag gtcaaaaaag gtgatgtgct gaaggcggta gtggtgcgca ccaagaaggg
     7681 tgttcgtcgc ccggacggtt ctgtcattcg cttcgatggt aatgcttgtg ttcttctgaa
     7741 caacaacagc gagcagccta tcggtacgcg tatttttggg ccggtaactc gtgagcttcg
     7801 tagtgagaag ttcatgaaaa ttatctctct ggcaccagaa gtactctaag gagcgaatca
     7861 tggcagcgaa aatccgtcgt gatgacgaag ttatcgtgtt aaccggtaaa gataaaggta
     7921 aacgcggtaa agttaagaat gtcctgtctt ccggcaaggt cattattgaa ggtatcaacc
     7981 tggttaagaa acatcagaag ccggttccgg ccctgaacca accgggtggc atcgttgaaa
     8041 aagaagccgc tattcaggtt tccaacgtag caatcttcaa tgcggcaacc ggcaaggctg
     8101 accgtgtagg ctttagattc gaagacggca aaaaagtccg tttcttcaag tctaacagcg
     8161 aaactatcaa gtaatttgga gtagtacgat ggcgaaactg catgattact acaaagacga
     8221 agtagttaaa aaactcatga ctgagtttaa ctacaattct gtcatgcaag tccctcgggt
     8281 cgagaagatc accctgaaca tgggtgttgg tgaagcgatc gctgacaaaa aactgctgga
     8341 taacgcagca gcagacctgg cagcaatctc cggtcaaaaa ccgctgatca ccaaagcacg
     8401 caaatctgtt gcaggcttca aaatccgtca gggctatccg atcggctgta aagtaactct
     8461 gcgtggcgaa cgcatgtggg agttctttga gcgcctgatc actattgctg tacctcgtat
     8521 ccgtgacttc cgtggcctgt ccgctaagtc tttcgacggt cgtggtaact acagcatggg
     8581 tgtccgtgag cagatcatct tcccagaaat cgactacgat aaagtcgacc gcgttcgtgg
     8641 tttggatatt accattacca ctactgcgaa atctgacgaa gaaggccgcg ctctgctggc
     8701 tgcctttgac ttcccgttcc gcaagtaagg tagggttact aaatggctaa gcaatcaatg
     8761 aaagcacgcg aagtaaaacg cgtagcttta gctgataaat acttcgcgaa acgcgctgaa
     8821 ctgaaagcga tcatctctga tgtgaacgct tccgacgaag atcgttggaa cgctgttctc
     8881 aagctgcaga ctctgccgcg tgattccagc ccgtctcgtc agcgtaaccg ctgccgtcaa
     8941 acaggtcgtc cgcatggttt cctgcggaag ttcgggttga gccgtattaa ggtccgtgaa
     9001 gccgctatgc gcggtgaaat cccgggtctg aaaaaggcta gctggtaatt gtcaccaatt
     9061 gaatcacggg agtaaagaca gatgagcatg caagatccga tcgcggatat gctgacccgt
     9121 atccgtaacg gtcaggccgc gaacaaagct gcggtcacca tgccttcctc caagctgaaa
     9181 gtggcaatcg ccaacgtgct gaaggaagaa ggttttattg aagattttaa agttgaaggc
     9241 gacaccaagc ctgaactgga acttactctg aagtatttcc agggcaaagc tgttgtagaa
     9301 agcattcagc gtgtcagccg cccaggtctg cgcatctata aacgtaaaga tgagctgccg
     9361 aaagttatgg cgggtctggg tatcgcagtt gtttctacct ctaaaggtgt tatgactgat
     9421 cgtgcagcgc gccaggctgg tcttggtggc gaaattatct gctacgtagc ctaatcggag
     9481 gaaaaaatgt ctcgtgttgc taaagcaccg gtcgttgttc ctgccggcgt tgacgtaaaa
     9541 atcaacggtc aggttattac gatcaaaggt aaaaacggcg agctgactcg tactctcaac
     9601 gatgctgttg aagttaaaca tgcagataat accctgacct tcggtccgcg tgatggttac
     9661 gcagacggtt gggcacaggc tggtaccgcg cgtgccctgc tgaactcaat ggttatcggt
     9721 gttaccgaag gcttcactaa gaagctgcag ctggttggtg taggttaccg tgcagcggtt
     9781 aaaggcaatg tgattaacct gtctctgggt ttctctcatc ctgttgacca tcagctgcct
     9841 gcgggtatca ctgctgaatg tccgactcag actgaaatcg tgctgaaagg cgctgataag
     9901 caggtgatcg gccaggttgc agcggatctg cgcgcctacc gtcgtcctga gccttacaaa
     9961 ggcaaggggg ttcgttacgc cgacgaagtc gtgcgtacca aagaggctaa gaagaagtaa
    10021 ggtaacacta tggataagaa atctgctcgt atccgtcgtg cgacccgcgc acgccgcaag
    10081 ctccaggagc tgggcgcaac tcgcctggtg gtacatcgta ccccgcgtca catttacgca
    10141 caggtaattg caccgaacgg ttctgaagtt ctggtagctg cttctactgt agaaaaagct
    10201 atcgctgaac aactgaagta caccggtaac aaagacgcgg ctgcagctgt gggtaaagct
    10261 gtcgctgaac gcgctctgga aaaaggcatc aaagatgtat cctttgaccg ttccgggttc
    10321 caatatcatg gtcgtgtcca ggcactggca gatgctgccc gtgaagctgg ccttcagttc
    10381 taaggtagag gtgtaagatg gctcacatcg aaaaacaagc tggcgaactg caggaaaagc
    10441 tgatcgcggt aaaccgcgta tctaaaaccg ttaaaggtgg tcgtattttc tccttcacag
    10501 ctctgactgt agttggcgat ggtaacggtc gcgttggttt tggttacggt aaagcgcgtg
    10561 aagttccagc agcgatccag aaagcgatgg aaaaagcccg tcgcaatatg attaacgtcg
    10621 cgctgaataa cggcactcta caacaccctg ttaaaggtgt tcacacgggt tctcgcgtat
    10681 tcatgcagcc ggcttccgaa ggtaccggta tcatcgccgg tggtgcaatg cgcgccgttc
    10741 tggaagtcgc tggggttcat aacgttctgg ctaaagcata tggttccacc aacccgatca
    10801 acgtggttcg tgcaactatt gatggcctgg aaaatatgaa ttctccagaa atggtcgctg
    10861 ccaagcgtgg taaatccgtt gaagaaattc tggggaaata aaccatggca aagactatta
    10921 aaattactca aacccgcagt gcaatcggtc gtctgccgaa acacaaggca acgctgcttg
    10981 gcttgggtct gcgtcgtatt ggtcacaccg tagagcgcga ggatactcct gctattcgcg
    11041 gtatgatcaa cgcggtttcc ttcatggtta aagttgagga gtaagagatg cgtttaaata
    11101 ctctgtctcc ggccgaaggc tccaaaaagg cgggtaaacg cctgggtcgt ggtatcggtt
    11161 ctggcctcgg taaaaccggt ggtcgtggtc acaaaggtca gaagtctcgt tctggcggtg
    11221 gcgtacgtcg cggtttcgag ggtggtcaga tgcctctgta ccgtcgtctg ccgaaattcg
    11281 gcttcacttc tcgtaaagca gcgattacag ccgaagttcg tctatctgac ttggctaaag
    11341 tagaaggcgg tgttgtagac ctgaacacgc tgaaagcggc taacattatc ggtatccaga
    11401 tcgagttcgc gaaagtgatc ctggctggcg aagtaacgac tccggtaact gttcgtggcc
    11461 tgcgtgttac taaaggcgct cgtgctgcta tcgaagctgc tggcggtaaa atcgaggaat
    11521 aagtagcaga tggctaaaca accgggatta gattttcaaa gtgccaaagg tggcttaggc
    11581 gagctgaaac gcagactgct gtttgttatc ggtgcgctga ttgtgttccg tattggctct
    11641 tttattccga tccctggtat tgatgccgct gtacttgcca aactgcttga gcaacagcga
    11701 ggcaccatca ttgagatgtt taacatgttc tctggtggtg ctctcagccg tgcttctatc
    11761 tttgctctgg ggatcatgcc gtatatttcg gcgtcgatca ttatccagct gctgacggtg
    11821 gttcacccaa cgttggcaga aattaagaaa gaaggggagt ctggtcgtcg taagatcagc
    11881 cagtacaccc gttacggtac tctggtgctg gcaatattcc agtcgatcgg tattgctacc
    11941 ggtctgccga atatgcctgg tatgcaaggc ctggtgatta acccgggctt tgcattctac
    12001 ttcaccgctg ttgtaagtct ggtcacagga acgatgttcc tgatgtggtt gggcgaacag
    12061 atcactgaac gaggtatcgg caacggtatt tcaatcatta tcttcgccgg tattgtcgcg
    12121 ggactcccgc cagccattgc ccatactatc gagcaagcgc gtcaaggcga cctgcacttc
    12181 ctcgtgttgc tgttggttgc agtattagta tttgcagtga cgttctttgt tgtgtttgtt
    12241 gagcgtggtc aacgccgcat tgtggtaaac tacgcgaaac gtcagcaagg tcgtcgtgtc
    12301 tatgctgcac agagcacaca tttaccgctg aaagtgaata tggcgggggt aatcccggca
    12361 atcttcgctt ccagtattat tctgttcccg gcgaccatcg cgtcatggtt cgggggcggt
    12421 actggttgga actggctgac aacaatttcg ctgtatttgc agcctgggca accgctttat
    12481 gtgttactct atgcgtctgc aatcatcttc ttctgtttct tctacacggc gttggttttc
    12541 aacccgcgtg aaacagcaga taacctgaag aagtccggtg catttgtacc aggaattcgt
    12601 ccgggagagc aaacggcgaa gtatatcgat aaagtaatga cccgcctgac cttggttggt
    12661 gcgctgtata ttacctttat ctgcctgatc ccggagttca tgcgtgatgc aatgaaagta
    12721 ccgttctact tcggtgggac ctcactgctt atcgttgttg tcgtgattat ggactttatg
    12781 gctcaagtgc aaactctgat gatgtccagt cagtatgagt ctgcattgaa gaaggcgaac
    12841 ctgaaaggct acggccgata attggtcgcc cgagaagtta cggagagtaa aaatgaaagt
    12901 tcgtgcttcc gtcaagaaat tatgccgtaa ctgcaaaatc gttaagcgtg atggtgtcat
    12961 ccgtgtgatt tgcagtgccg agccgaagca taaacagcgc caaggctgat tttttcgcat
    13021 atttttcttg caaagttggg ttgagctggc tagattagcc agccaatctt ttgtatgtct
    13081 gtacgtttcc atttgagtat cctgaaaacg ggcttttcag catggaacgt acatattaaa
    13141 tagtaggagt gcatagtggc ccgtatagca ggcattaaca ttcctgatca taagcatgcc
    13201 gtaatcgcat taacttcgat ttatggcgtc ggcaagaccc gttctaaagc catcctggct
    13261 gcagcgggta tcgctgaaga tgttaagatc agtgagctgt ctgaaggaca aatcgacacg
    13321 ctgcgtgacg aagttgccaa atttgtcgtt gaaggtgatc tgcgccgtga aatcagcatg
    13381 agcatcaagc gcctgatgga tcttggttgc tatcgcggtt tgcgtcatcg tcgtggtctc
    13441 ccggttcgcg gtcagcgtac caagaccaac gcacgtaccc gtaagggtcc gcgcaaaccg
    13501 atcaagaaat aatcggggtg attgaataat ggcaaaggca ccaattcgtg cacgtaaacg
    13561 tgtaagaaaa caagtctctg acggcgtggc tcatatccat gcttctttca acaacaccat
    13621 cgtgactatc actgatcgtc agggtaacgc gttgggttgg gcaacagccg gtggttccgg
    13681 tttccgtggt tctcgcaaat ccactccgtt tgcagctcag gttgcagcag agcgttgcgc
    13741 tgacgccgtg aaagaatacg gcatcaagaa tctggaagtt atggttaaag gtccgggtcc
    13801 aggccgcgaa tctactattc gtgctctgaa cgccgcaggt ttccgcatca ctaacattac
    13861 tgatgtgact ccgatccctc ataacggttg tcgtccgccg aaaaaacgtc gcgtataacg
    13921 cctcgttttc caggtttgtt ggagaaagaa aatggcaaga tatttgggtc ctaagctcaa
    13981 gctgagccgt cgtgagggca ccgacttatt ccttaagtct ggcgttcgcg cgatcgatac
    14041 caagtgtaaa attgaacaag ctcctggcca gcacggtgcg cgtaaaccgc gtctgtctga
    14101 ctatggtgtg cagttgcgtg aaaagcaaaa agttcgccgt atctatggtg tgctggagcg
    14161 tcagttccgt aactactaca aagaagcagc acgtctgaaa ggcaacaccg gtgaaaacct
    14221 gttggctctg ctggaaggtc gtctggacaa cgttgtatac cgtatgggct tcggtgccac
    14281 tcgtgcagaa gcacgtcagc tggttagcca taaagcaatt atggtaaacg gtcgtgttgt
    14341 taacatcgct tcttatcagg ttagtccgaa tgacgttgta agcattcgtg agaaagcgaa
    14401 gaagcagtct cgcgtgaaag ccgctctgga gctggctgag cagcgtgaaa agccaacttg
    14461 gctggaagtt gatgctggca agatggaagg tacgtttaag cgtaagccgg agcgttctga
    14521 tctgtctgcg gacattaacg aacacctgat cgtcgagctt tactccaagt aaagcttagt
    14581 accaaagaga ggacacaatg cagggttctg tgacagagtt tctaaaaccg cgcctggttg
    14641 atatcgagca agtgagttcg acgcacgcca aggtgaccct tgagccttta gagcgtggct
    14701 ttggccatac tctgggtaac gcactgcgcc gtattctgct ctcatcgatg ccgggttgcg
    14761 cggtgaccga ggttgagatt gatggtgtac tacatgagta cagcaccaaa gaaggcgttc
    14821 aggaagatat cctggaaatc ctgctcaacc tgaaagggct ggcggtgaga gttcagggca
    14881 aagatgaagt tattcttacc ttgaataaat ctggcattgg ccctgtgact gcagccgata
    14941 tcacccacga cggtgatgtt gaaatcgtca agccgcagca cgtgatctgc cacctgaccg
    15001 atgagaacgc gtctattagc atgcgtatca aagttcagcg cggtcgtggt tatgtgccgg
    15061 cttctacccg aattcattcg gaagaagatg agcgcccaat cggccgtctg ctggtcgacg
    15121 catgctacag ccctgtggag cgtattgcct acaatgttga agcagcgcgt gtagaacagc
    15181 gtaccgacct ggacaagctg gtcatcgaaa tggaaaccaa cggcacaatc gatcctgaag
    15241 aggcgattcg tcgtgcggca accattctgg ctgaacaact ggaagctttc gttgacttac
    15301 gtgatgtacg tcagcctgaa gtgaaagaag agaaaccaga gttcgatccg atcctgctgc
    15361 gccctgttga cgatctggaa ttgactgtcc gctctgctaa ctgccttaaa gcagaagcta
    15421 tccactatat cggtgatctg gtacagcgta ccgaggttga gctccttaaa acgcctaacc
    15481 ttggtaaaaa atctcttact gagattaaag acgtgctggc ttcccgtgga ctgtctctgg
    15541 gcatgcgctt ggaaaactgg ccaccggcaa gcatcgctga cgagtaaccg gatcacaggt
    15601 taaggtttta ctgagaagga taaggtcatg cgccatcgta agagtggtcg tcaactgaac
    15661 cgcaacagca gccatcgcca ggctatgttc cgcaatatgg caggttcact ggttcgtcat
    15721 gaaatcatca agacgactct gcctaaagcg aaagagctgc gccgcgtagt tgagccgctg
    15781 attactcttg ccaagactga tagcgttgct aatcgtcgtc tggcattcgc ccgtactcgt
    15841 gataacgaga tcgtggcaaa actgtttaac gaactgggcc cgcgtttcgc gagccgtgcc
    15901 ggtggttaca ctcgtattct gaagtgtggc ttccgtgcag gcgacaacgc gccgatggct
    15961 tacatcgagc tggttgatcg ttcagagaaa gcagaagctg ctgcagagta atctgaagca
    16021 atgtaaaaaa acccgccccg gcgggttttt ttatacccgt agtatcccca cttatctaca
    16081 atagctgtac tctttt
//
LOCUS       DAZPCS010000084        15904 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZPCS010000084 DAZPCS010000000
VERSION     DAZPCS010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15904
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.084"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..756
                     /locus_tag="ACHUQ7_002553"
     CDS             <1..756
                     /locus_tag="ACHUQ7_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease family protein"
                     /protein_id="HFR1135912.1"
                     /translation="YTKILENDFEHPSIRRKIASLSNLGVDEWIPPVMAFMNRMARTE
                     DFNLDDFSQFITAFEKVYMHGWLKKQIKSQREMVCYSALVAINNDMPFDSVINQINQH
                     ADNSGFIAALDEDLYEPRPNQVNLIKAILLRLDMEQQDESVIKTYTGRITIEHILPQA
                     LVNEYWINRFQPQEHVYWLHKIGNLTLISGSKNSETQHYDFIKKKSIYEKLNSKSSFY
                     LTKDVCNSSEWGLAELKMRHEKMKTQLKKLWLV"
     gene            complement(825..1781)
                     /gene="yjiA"
                     /locus_tag="ACHUQ7_002554"
     CDS             complement(825..1781)
                     /gene="yjiA"
                     /locus_tag="ACHUQ7_002554"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFR1135913.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQLYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(1792..1995)
                     /locus_tag="ACHUQ7_002555"
     CDS             complement(1792..1995)
                     /locus_tag="ACHUQ7_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFR1135914.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            complement(2045..4195)
                     /gene="btsT"
                     /locus_tag="ACHUQ7_002556"
     CDS             complement(2045..4195)
                     /gene="btsT"
                     /locus_tag="ACHUQ7_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFR1135915.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            complement(4588..5100)
                     /locus_tag="ACHUQ7_002557"
     CDS             complement(4588..5100)
                     /locus_tag="ACHUQ7_002557"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HFR1135916.1"
                     /translation="MQLDEQRLRFHDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            complement(5118..6676)
                     /gene="hpaB"
                     /locus_tag="ACHUQ7_002558"
                     /pseudo
     CDS             complement(5118..6676)
                     /gene="hpaB"
                     /locus_tag="ACHUQ7_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016712 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, reduced flavin or flavoprotein as one
                     donor, and incorporation of one atom of oxygen [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
     gene            complement(6903..7840)
                     /locus_tag="ACHUQ7_002559"
                     /pseudo
     CDS             complement(6903..7840)
                     /locus_tag="ACHUQ7_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601398.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            complement(7867..8757)
                     /gene="hpaA"
                     /locus_tag="ACHUQ7_002560"
     CDS             complement(7867..8757)
                     /gene="hpaA"
                     /locus_tag="ACHUQ7_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HFR1135917.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFARLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSLEGMEHTLAALNHYWPIISLEFQQNKQG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDENYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCSPTQFRGR"
     gene            complement(8767..10122)
                     /gene="hpaX"
                     /locus_tag="ACHUQ7_002561"
     CDS             complement(8767..10122)
                     /gene="hpaX"
                     /locus_tag="ACHUQ7_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901241 - 4-hydroxyphenylacetate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1900754 - 4-hydroxyphenylacetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HFR1135918.1"
                     /translation="MTTSTLQYKDNKTVEIEHRVINKLFRRLIIFLFILFVFSFLDRI
                     NIGFAGLTMGKDLGLTSTMFGLAATLFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVW
                     GIASTCTMFATSPETLYLLRMLVGIAEAGFLPGILVYLTWWFPAYHRARANALFMIAM
                     PVTMMLGLILSGYILALDGLWNLKGWQWLFLLEGLPSVILGVVTWFYLNDTPDQATWL
                     DENEKQALKTMILREQESAFAHASVPRSTLREVLTPAIMLYTLAYFCPTNTLSAINIW
                     TPQILQSFNTGSSNIMIGLLAAIPQFCTIFGMIWWSRRSDRLKERKKHTILPYLFAAA
                     GWLLASATDHSLIQLSSIIMASMGSFTAMTIFWTTPDQVISLQSRAVAMAVINAVGNV
                     GSAVSPLLIGILKDATGSFSSGLWFVAGLLVLGALVMTRIPMGSREHSLSSELIAPKP
                     Q"
     gene            complement(10144..10947)
                     /gene="hpaI"
                     /locus_tag="ACHUQ7_002562"
     CDS             complement(10144..10947)
                     /gene="hpaI"
                     /locus_tag="ACHUQ7_002562"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018802 -
                     2,4-dihydroxyhept-2-ene-1,7-dioate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HFR1135919.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGNSSSISMAKQNNNSVY"
     gene            complement(10958..11761)
                     /gene="hpaH"
                     /locus_tag="ACHUQ7_002563"
     CDS             complement(10958..11761)
                     /gene="hpaH"
                     /locus_tag="ACHUQ7_002563"
                     /EC_number="4.2.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hept-4-ene-1,7-dioate hydratase"
                     /protein_id="HFR1135920.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAESRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLETGQIILGGSFTLPVPARKGDTFHVDYGNMGSISCRFV"
     gene            complement(11829..12209)
                     /locus_tag="ACHUQ7_002564"
     CDS             complement(11829..12209)
                     /locus_tag="ACHUQ7_002564"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HFR1135921.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            complement(12219..13070)
                     /gene="hpaD"
                     /locus_tag="ACHUQ7_002565"
     CDS             complement(12219..13070)
                     /gene="hpaD"
                     /locus_tag="ACHUQ7_002565"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HFR1135922.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            complement(13072..14538)
                     /gene="hpaE"
                     /locus_tag="ACHUQ7_002566"
     CDS             complement(13072..14538)
                     /gene="hpaE"
                     /locus_tag="ACHUQ7_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HFR1135923.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALASPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            complement(14535..15824)
                     /gene="hpaG"
                     /locus_tag="ACHUQ7_002567"
     CDS             complement(14535..15824)
                     /gene="hpaG"
                     /locus_tag="ACHUQ7_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008704 -
                     5-carboxymethyl-2-hydroxymuconate delta-isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0018800 -
                     5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HFR1135924.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALDNDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPSDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
BASE COUNT         3745 a         4149 c         4163 g         3847 t
ORIGIN      
        1 tatactaaga ttttagagaa tgattttgaa cacccttcaa tacgacgaaa aatagcatcg
       61 ctaagtaatt taggggtaga tgagtggatt cctccggtta tggcattcat gaatcgaatg
      121 gctcgtacag aagattttaa tttagatgat ttttctcaat ttattaccgc ctttgagaag
      181 gtatatatgc atgggtggtt aaaaaaacaa attaaaagcc aaagagaaat ggtttgttat
      241 tctgctctgg tagcaataaa taatgatatg ccttttgatt ctgtgataaa tcagataaac
      301 caacatgcag ataattcagg ttttatagct gctctggatg aagatttata tgagcctcgt
      361 ccgaatcaag tgaacttaat aaaagcaatt ttgcttcgct tagatatgga gcaacaagat
      421 gagagcgtga ttaaaaccta tacggggaga atcacaattg agcatattct tcctcaagca
      481 ttagttaatg aatactggat taatagattc caacctcaag agcatgtcta ctggctgcat
      541 aaaataggta atttaacact cataagtggt tccaaaaatt ctgaaacaca acactatgat
      601 tttatcaaaa agaaatctat ttatgagaaa cttaatagta aaagctcttt ttatttaaca
      661 aaagatgttt gtaattcttc agaatgggga ttggctgaac tgaaaatgag acatgaaaaa
      721 atgaaaacgc aattgaagaa gctttggttg gtctgacata tctaaagagt tccactcgta
      781 ataatgttgc ggtggaactc tcaaaaagtt gatctttccc gaacttactt cctcaatccc
      841 gcaaacgccg cccgaatctc ttcctccggc aactgaatcc cgataaacac cattgtgcta
      901 tgcggttttt catcgcccca cggcctgtcc cagtcggcgc tgtagaggcg ctggacgccc
      961 tggaacagca ggcggttagg ttcgccgtca atccacagca tccctttgta acgcagcagt
     1021 ttatccgccg attccagcag caggttttcc atcacgcggg aaacttcgct gatatctacc
     1081 gggtaatcca gttccaccac aattgacgaa atatcgtttt gtttatccgc gataaagtgg
     1141 aaacgcggtt tggtgctgac gacgttctct tccagcataa aaccgttggt gttgaacagc
     1201 aggcccaggt cgatgtcgcc gtgggtgacg gtgtagaccg gtgcgcgggc gttgatgcgc
     1261 gccaggcgtt cacgcagttt ctctgcttcg cctgcgacgt cggttttagt cagcagaata
     1321 cggtcggcgt agcccacctg ggactgggcg atggtgaact ggttcatctg ctcatcggca
     1381 tgtaccgcat ccaccagcgc aatcacgccg tccagcaggt agagctggca taaaatctca
     1441 tgggagaaaa aggtctgaat aatcgggccg ggatcggcca tgccggtgca ttcaatcacc
     1501 agacgatcga actgaatatt gcccttgtcg agattatcca gcaggtcgag tagtgcgtcc
     1561 tccagctcgt tggagcgcga acagcagatg cagccgttgg tcagcgtttt gatctgcgtg
     1621 gcgcggtcgc caatcagttg gtcatcaacg gagacttcgc cgaattcgtt ttcaatcacg
     1681 gcaatcttgt agccgtgttg ttcgttaaga atatggcgca gcagggtagt tttgcccgcg
     1741 cctaaaaaac cggtgagtag ggtaactgca atcgggttca tgctctctcc attaacaaca
     1801 gcgcataccg cctttaccat cgccgccgta gcgcgcgttc tggcgttcgc ggaagaattc
     1861 ttcatagctc atgtacggct tgtcgggatg gttggtcttc atatgctcga cgtagttgtc
     1921 gtagtctgga ataccaatca acatcttcgc cgcctggccg agatattttt ttgcctgtcc
     1981 taagttacca aacatagttc actctgataa gaacaaagcc ccgccgaagc ggggctaaac
     2041 acggttagtg gtgcgaagag atcttcacgc cgccttccgg gattggaacg tacggtgttt
     2101 ctttgtcagt acgtttgtcg ctgttacgca ccgcaagcca ggttttgaaa ccgtagaaga
     2161 tgatgctgta caccacaatc aggaacagaa tactcaggcc tgcgttggtg tagttgttca
     2221 caacgatgtg gttcatgttg gcaatctgct gcgccgtcag gtcagtaccg ttagcaatct
     2281 tctctttgta ctggcttgcc atgtagaaga agccttccat ctgcgggttg gtgctgaaca
     2341 gtttcagacc cagcgcccag gtggtgcaga taagcagcca tacagccgga acaacagtta
     2401 cccagatgta ttgggtgcgc ttcatcttaa tcagcacaac ggtgcccagt accagcgcta
     2461 cggctgccag catctggttg gagataccga acagcggcca caggctctta acgccgccca
     2521 gcggatcgac cacgccctga tacagcaggt agccccacag acccacacag cccgcagtac
     2581 cgatgatacc ggcaaccaga gagtcggttt ttttcaggaa cgggatgaag ttacccagca
     2641 ggtcttgcag cataaagcgg ccagaacggg tacccgcatc cagcgcggtc aggatgaaca
     2701 gggcttcgaa cagaataccg aagtgatacc agaagcccat gtcagccatc ggcagcactt
     2761 tgtggaacac gtgagcgata cctaccgcca gcgtcggcgc accacctgca cggttcagga
     2821 cagaaggctc accaatgtct ttcgcggttt gcaggatctg ctctggcgaa atcacgaagc
     2881 cccaggagct gacggtcgct gccgcgtgtg cggtaacgtc tttcagctgc gccatgatga
     2941 tcggcgcgtt ctcgccaccc atttcatgca ggttaggcat ggtgatgcca aggccagcag
     3001 gcggggtgtt catcgcgaag taaagacccg gttcgatgat ggacgctgca accagcgcca
     3061 taatcgccac gaaggactcc atcagcattg cgccgtagcc aatgaaacgc gcgtcggttt
     3121 cgttagccag cagtttcggc gtcgtaccgg aagagatcag cgcgtggaag ccagataccg
     3181 caccacaggc gatggtgatg aacaggaacg ggaacagagc gcctttccac agcgggccag
     3241 taccgtcaat gtactgggtc atcgcaggca ttttcagttc cgggttcagc accacgatac
     3301 ccagcgccag gccgacgata acgccgattt tcaggaaggt tgccagatag tcgcgcggtg
     3361 cgaggatcag ccacaccggc agcagtgcgg aaacaaacgc atagccaatc agcgcgaagg
     3421 taatggtggt gtctttaaag gtcagtgccg gaccccagta cggatcgtga gcaatcacgc
     3481 caccgaagta gatagaggca accagcagca cgataccaat aacagagact tcacccacac
     3541 gccccggacg gataaagcgc atgtagatac ccataaacag cgcaatcggt acggttgagc
     3601 aaacggtgaa gacaccccac ggactttcgg ccagggcttt aaccacaatc agcgccagaa
     3661 cggcgaggat gatgatcatg attaagaaac agccaaacag cgcgatagtc cccggtactg
     3721 gccccatctc ttctttgatc atctcaccga gagatgcgcc gttacggcga gaggagataa
     3781 acagcaccat aaagtcctgt accgcaccgg ccagcactac ccccgccagc agccacagcg
     3841 tgccaggcag gtagcccatc tgcgcggcga gaaccggtcc caccagcgga ccagcaccgg
     3901 cgatagcggc gaagtggtga ccaaacaaca cgtaacggtt ggtcggaacg tagttcagac
     3961 cgtcgttgtt aataaccgca ggcgtcgcgc gcgtggggtc gagtttcatc accttctggg
     4021 cgatgtacag actgtagtag cgatacgcca ccagatacac cgacacagag gcgaccacga
     4081 tccacagggc gctgacgtgc tccccccgac gtaatgcaac taccgcgagg cagaatgcac
     4141 cgatgattcc gagaatcacc cagggtatgt gcttgaatag ttttttcgta tccatagtaa
     4201 aacctggcat tttactcgtc gtcttttggc ttacaggtgc ggcagctgcg ttcgttcacc
     4261 tctgacactt acttttgtaa gctcccgggg attcactcac ttgctgcctt cctgcaaacc
     4321 aaaatccata gagtaatttg ttaatcatag gccgaagccg ttctaggttt gcgttgcgtt
     4381 tgaggaggta aattgaccgc tatcctgcag tgatgttgcc agagatacgt gcgcgtaaag
     4441 taaggtaaat aactgagtgg ttattttagg gatgtaagcg gtcaggaatg gcggtgagcg
     4501 gttgggcaag aattattcat cgggtagata tgtgattgag atcacaataa aaatgcccct
     4561 tcgacgaatg cgtgaagggg ctggagctta aatcgcagct tccatttcca gcatcaccgg
     4621 atggaaacgg cgtttaaagt aaataagtcc gtgaccttct gcactgagga tgatgttttt
     4681 aatctccacc agatacacca gatgtgtgcc aatcgcctgc acatcgcgga tctctccttc
     4741 aagactggcc agcgaacctt ttagcaccgg ctgcgccagc ggaccttttt gccagcatga
     4801 gaggctaaaa cgctcttcca tcgccatgcc tgtcatgccc gcgaagtggc gcgccatcag
     4861 ttcctgctca tggttgagga cgttgacgca caacttgccg ttgccctgaa aaaccgggtt
     4921 catcgcactg ttggcgttaa tgcacaccat cagcgacggt ggtgtatccg tgaccgagca
     4981 gacagccgtt gccgtaatcc cgcattgtcc ggcgtcgccc tcggtggtga taatatttac
     5041 cgctgccgac aggctggcca tcgcgtcatg aaagcgcagg cgttgttcat ctaattgcat
     5101 cttaacctcc tgctgcgtta tttcagcagc ttatccagca tgttgatatc gtcgttgttg
     5161 tgcaggtgcg gcacagtcca gccgttctgg tcgtattccg acaggcagcg atcaaccatc
     5221 gccatcatct tgtccatatt gccggagctt tgtgcctggc gcagacattg caggcgaatc
     5281 tcatcctggc taccggagta gttgatttca tacagctcgt gacgaccacc aaattcgctg
     5341 ccaatggcgt cccacatcag tttgaggatc ttgatgcgct ggacgtgatc cataccgttc
     5401 gaaccgcgca catacttcgc cagatactgg tcgatctgcg gattgttcag gtcacgggca
     5461 ctggaaggga ggtagatcag gccactggta acgttgcgtt ctataatgtt tttgatcttc
     5521 gcgtaggcca ttggtgccag tacgcgatag gtttgcagtg cggcatgatc cggtaaataa
     5581 gccccgttga cccacggcgt cgcttctgaa cacatcgagt cactcaatgc ccagaaggtg
     5641 ttgcgccacg ccaccacttc accgagatcg gcctgcacac cacggaactc cagggtgccg
     5701 gtacattcga gtgatttttt cagcagtgcc gtaatgaagt cgagtttcac tgccaggcgc
     5761 acacaggctt gcagcggata catacgggcg aaaccgcctt ccatcgtcca gcgacggcag
     5821 cgatcaaaat cgcggtagat cagcacgttt tcccatggga tcagtacgtt atccatcacc
     5881 agaatcgcat cgttctcatc gaagcggctg gagagcgggt agtcgtatgg cgagccggta
     5941 gcacccgcga ccatctcata agaggcgcgg gagattaatt tcacgccatc ggcatccatt
     6001 ggcgcaacga acatcagtgc gaagtccggg ttttcgccca tcacttgtgc cgagccgaag
     6061 ccaatcatgt tgtagtgagt cagcgccgag ttggtggcaa ccactttcgc accgctgacg
     6121 ataatcccgg cgtcagtctc tttttccagc ttgatgtaaa cgtctttcac tttatcggtc
     6181 ggcaaatgac gatcggtggg ttaacaatcg cgtggttaaa gtagaggcca gtttcctgaa
     6241 tacgggtata ccagttacgg gcgttctgct cgaactgacc gtaaaagccc ggattcgcgc
     6301 ccagtgcgca accgaaagcg gctttgtagt ctggggtacg gcccatccag ccatagctca
     6361 ggcgtgacca ttcagcgatg gcgtcgcgtt gctggcgcag gtcgtcggca cttttcgcca
     6421 cgcggaagaa tttatgggta tagccgccgc tgccggtgtc ggtgttccaa cacagagagt
     6481 cctgcatctc cggtttgtgc agcgcgtcgt acagttgggc aacagacgct gccgcattac
     6541 gaaatgccgg atgagtggtg acgtctttca ctcgctcgcc atagatatag atctcgcgac
     6601 catcctgcag gcttttcaga tactcttccc cggtgaaagg acgttgggta ctggcgcgga
     6661 aatcttctgg tttcatgtcg acctctacag tattaatcgg aatacgattg ttaattttgt
     6721 gtgttgtttt tgttatttaa ttgaagcgtt gaacgcggtt gtcgggaaga gacgtttcag
     6781 gcgaacgttg gtacttttcg acagcaaggt ggagttaaat agtaaactgc gatcgtgatc
     6841 aacttacagt aaggcaaatc ataatattcc ccgctaacaa gggctaacgg ggacggaatt
     6901 ttttatgggg cgaatggaat ggtaatcgtc atattttcat gaccaacgac gataggacct
     6961 gaatagtttt ctgtgcttct tgcttccatg tttcatccgg aacggtgatt ggtaccaggt
     7021 ggctaaggat gagttttttc acttttgctt cattggcaat tttgcctaca tcctcaatag
     7081 tcgtatggtg agaagcaatg gcttttgcca gtgttttacc attgccaata ctgtctgcca
     7141 gcttagcgac ggagggctca tgtacggcct catgcacaag tatatcagcg ccttctgaga
     7201 aagttgccag tgggggaaag tacgaggtat ctcctgagaa gacaattcgc ttgccttgtg
     7261 tgtcgaagcg ataagcaaaa gcctcaccgt cagggaaagg agaatgaggg acctttaatg
     7321 cagtcacggt cacggtatca tctttataaa ctacgccttc accaacatct tgagctgtaa
     7381 cgagtttgcg aggatcgggt tttccttctt cttccatacg cagtgtgatg tcccgatcga
     7441 agacctgcca actggcagcg gtcatggctc gggtgcctgg cggaccgaat acatgaatag
     7501 ttttgtcctg cagaccgcta gcccatgcat tcattaagac tgatggatag tccagaatat
     7561 gatcactgtg taaatgggtg agtaaaatat tatcaatctg gcgcagtgga atattatttt
     7621 ttaccagttg taagctggcc ccgtaaccag catcaattaa ccaggtttta ttgccaataa
     7681 taagtgccgt tgccatcggt aaacggtcag cttttaataa agaagggcca cctttcgttc
     7741 caagtagtac aattttcatt tattgcggtt cggaggcgta ggtgaatgcc actggcgcaa
     7801 tcaggcacgc cacaaatagt gagcgtaaag atattttcat gtcgagtctc tcaggttgat
     7861 aaatggtcag cgaccacgga attgtgttgg tgagcaaccc atcattcggt tgaaaaaacg
     7921 ggcgaaatag gcgggatctt tgtagccaag atcaagggct atctggttga tgctttgctc
     7981 gctatagagc agaagccgtc tggcttcccg gctcactcgt tcgaaaatca atctcttcgg
     8041 cgattgattg gcaaaacgtc ggcacagctc ggtaagccgt gattcactca taccgagcat
     8101 tgttgcatat tcagggacca ttaaatgttg gcgataattt tcgtcaatca gccgattaaa
     8161 cttctggaat aaacacatct cgccgcgcac gctgcatgtt gaaatatcat ttggtggtgc
     8221 ttcacgcagt aggattgtaa aaatactttg cgccagtgat tgcaatagaa tttcccgtcc
     8281 ttgcttattt tgttgaaact ccagggatat tatcggccaa taatgattta atgccgccag
     8341 tgtatgttcc attccctcca gtgacagaca gataccttgt agcattgcgg catctccact
     8401 accaggccag agcttttcta ccaggggcca aattaactcc tgacgtacgg tcagaacatg
     8461 cccatcgcta tcaggatcag taaagaaagt atgtggcacc gatggtggtg ttagtacaaa
     8521 aagcggtgcg cgcacagaat agaagtgctc atccagttgt aaagtaatgc gtccagtgac
     8581 cagataatgg agttgaaaat atctatcgtg ccagtgaggg cgcatatcgc gtccaaagaa
     8641 attcgctaac cgggcaaatg tttcataatg cacatcttca ccagcgtagc gggcatcata
     8701 ttcccggacc aaatcaatat tttcataacc atgttcaaag atagcttcac tggacatcat
     8761 gactccttat tgcggttttg gtgcgataag ttcagatgaa agagagtgtt cccgactccc
     8821 catagggatt cgggtcatga ctaacgcgcc cagtaccagc aatcctgcta caaaccacaa
     8881 acctgagcta aaactgccgg ttgcatcttt caaaataccg ataagtaatg gactgactgc
     8941 tgaacctaca ttgccgacag cattgatgac agccatcgca acggcacgcg attgcaagct
     9001 tataacttga tccggtgttg tccaaaagat agtcatcgcg gtaaatgatc ccattgaggc
     9061 cataatgatg ctgctcagtt gaatgaggct gtgatctgtc gcagaagcga gtaaccatcc
     9121 ggcagcggca aacaggtagg gaagaatcgt atgctttttg cgttctttaa gacggtcgga
     9181 acgacgactc caccagatca tgccaaaaat tgtgcagaat tgggggatgg ccgccagcaa
     9241 accaatcatg atgttgctac tgccagtatt gaaactctgt aggatttgcg gcgtccagat
     9301 attaatcgca ctcagagtat tagtcgggca aaaataagca agtgtatata gcatgatggc
     9361 tggggtcagg acttcacgta aggttgagcg tggaacagat gcatgggcaa atgcactctc
     9421 ttgttcacgg agaatcatcg tttttaaagc ctgtttttcg ttttcatcaa gccaggtcgc
     9481 ctggtctggt gtgtcgttga gataaaacca ggtgacgaca ccaagaatga ccgatggcag
     9541 accttccagc aaaaagagcc attgccagcc ttttagattc cacagtccat ccaaggccag
     9601 aatgtagcct gaaaggatca atcccagcat catagtgact ggcatcgcta tcataaataa
     9661 ggcattggct ctggcccgat gatatgccgg gaaccaccag gtcagataaa ccaaaatacc
     9721 aggtaaaaaa cccgcctcag caataccaac cagcatacgc agtagataga gtgtttcggg
     9781 gcttgttgca aacattgtgc aggtggaagc aattccccag accaccatga tcccagcgat
     9841 ccatcgacga gcgccaattt tcgctaacat catattactg gggatcccgc acatgacgta
     9901 cgtgacataa aagagtgttg cggcgaggcc aaacattgtg gacgtcaggc caagatcctt
     9961 gcccattgtc agtccggcaa atcctatatt gatgcgatca agaaacgaaa aaacaaacag
    10021 aataaaaaga aaaatgatta ggcgacgaaa taatttatta atgacacgat gttcaatttc
    10081 aacagtttta ttatctttat attgcagggt agaggtcgtc atgatgcgct ccacatcgta
    10141 cgtttagtag acagagttgt tgttttgctt cgccatcgaa atagaagaac tattaccaaa
    10201 ccgtgctgcc agcgcttcgg cggcgcgggc gagcagggtg gtgtcaacgc cgacggcgac
    10261 aaacagcgcg cccagttcca gatagcgttt tgccagttgc tcattggcga tcaggatccc
    10321 cggcgctttg cccgcttcgc ggatctgcac gatcgcctgc tcaatggcgg cctgtacttc
    10381 cgggtgctgc ggattaccgg cataacccat atcggcgctc agatccgccg ggccgataaa
    10441 cacgccgtcg accccttcca cgtccagaat ctgcggtaag ttcttcattg cctcacgcgt
    10501 ttcgatctgc accagcacgc acatttgatc gttggctttt tgcaggtaat caggaatgcg
    10561 attccagcgc gaggcgcgag ccagcgcact gcccacaccg cgaataccgg cggggggata
    10621 acgggtggcg cgtaccgctt tacgggcttc gtcggcgttt tgtaccatcg gcaccagtaa
    10681 ggtttgtgtg cctacgtcca gcagttgttt gatttgcacc ggatcgttcc acgatgggcg
    10741 taccactggc tggctggggt agggcgcaat cgcctgtagt tgggtgagca cggtttgcac
    10801 gttattcggc gcgtgctcac cgtcgatcaa taaccagtcg aatcctggtc cggccagtaa
    10861 ctccgcgctg tagctgctgc tcagccccag ccataatcca atctgcggac ggcctgcttt
    10921 cagcgccgct ttaaaactgt tttccatcgt tctctcctta aacaaagcgg cagctaatgg
    10981 agcccatgtt gccgtaatcg acgtggaagg tgtcgccctt acgcgccgga accgggaggg
    11041 tgaacgaacc accgagaatg atttgcccgg tttccagttg tacgtcatac ggtgcgagtt
    11101 tgttcgccag ccaggccacg ccgtttgccg gatgattcag cacaccagcg gcgacgccgg
    11161 tttcttcaat cacgccattg cgatacatca gggcggagat ccaacgcaga tccagttcgt
    11221 ctggtttgat cggacgacct ccgaggatca ctccggcgtt ggcggcgtta tcggaaatgg
    11281 tgtcgaatac tttgcgcggg cgctgggttt ccggatcgat gttgtggcag cgagcgtcga
    11341 tcagctccag cgccgggatc acatagtccg tggcgttgta aacgtcgaac agcgtgcagt
    11401 ttggcccacg cagcggtttt gccagcacaa aagccagctc cacttcaatg cgcggcacga
    11461 taaagcgatc ggtcgggata tcgctgccat cgtggaagaa catgtcgtcc agcagtgcac
    11521 cgtaatccgg ttcgctgatc tgcgagcttg cctgcatcgc tttcgaggtc agaccgattt
    11581 tgtggccttt cagcgtgcga ctttcggcga ttttcaggcg aacccattcg cgctgcacgg
    11641 cgtaggcgtc ttcgatggtg atctccgggt aatccagcga gatcgcgcgg atctgttcgc
    11701 gctgtttttc tgcctgatcc agacgctggg cgatcagggt gtgggtgtgt ttatcgaaca
    11761 tagcgatatc ctgtggcatt gccggatgcg gcagcgcctt atcatccggc ctggaagctg
    11821 gtgcaaattt atttaaacaa cgcgtgtacg ttgttctgtt tgaaattcaa cgtcgggtgc
    11881 agctctgcaa tctcaaatga aagcgctagg taacggctct ccatcagtga ggcgaagtgc
    11941 gttttaatca gatcaaacag catttcgccc acttcctgac ggctttccag actgcgtccg
    12001 ctgccgattt ttagcgtcat atgcacaaag gcgtaatcgt gcttgccatc agccatttgc
    12061 caggtatcga tccagtgcgc ccggctacgt attcccccaa gcggaaaaat ccccgttgcc
    12121 gccagtgcag tgttaacact ggcaaacagc tcaggtaagc gggcttcttc acgaatattt
    12181 tcagtacatt ccacgataaa gtgcggcata aaacgtcctt acgcgggcag cgggaaaaca
    12241 gcgttaacct gaccggtgcc agagcttggg aacagctcgg taataaactc caccttgccg
    12301 tcgtatttat cccagccgag catccccagc agcatcaccg tgtcgtgcat attgccttcg
    12361 ccgtagcagt agtcggcgta ctccggcagc atattgcaaa actctttgaa ctggccttcg
    12421 cgccacagct tcaccacacg ctcgtccatc tggcggtcga actcgcgggt gtagctgttc
    12481 atcccttctt ccgcacgctg atcgtcaata aagcggtgcg ataacgaacc gctggcaagg
    12541 accgccacgg tgccgtcgta ctgttcgatc gctttcagaa tcgcttcgcc cagcttgcgg
    12601 ctgtcggcaa aatcgtgaac cgtgcagaaa gccgaaatgg agaccacttt gaagtgcttg
    12661 tcttcattca tgtagcgcat cggtaccagc gtgccgtatt ccagtttcag gctgggaata
    12721 ttgtgcgctt ttgcccgcac gccgagcttt aaggcttcct cggcaattag ctgccccaac
    12781 tccgggttgc cctcgtagtt gtaggtcatg tcgcgaataa aatgcggcag ctcgttgctg
    12841 gtgtagacgc cttcaaaatg gtctgcacag ttgatgtgat aagcactgtt gaccagccag
    12901 tgagtatcga aaacgataat ggtatcgacg cccatttccc ggcaacgctt gctgatctct
    12961 ttatgcccgt cgatcgcacc ctggcgacaa ccgtggtttt ttcctggtag ttcagagaga
    13021 tacatcgacg gtacgtgcgt gatcttggcg gctaacgcta acttacccat atcagactcc
    13081 ccatttcgga attggatggt cgcccatgga aatgcagacg ttcttcattt ccgcgaacac
    13141 ttcgaagctg tactcaccgc cttcacgccc ggtgccggag gcttttacgc cgccaaatgg
    13201 ctggcgcagg tcgcgcacgt tctgggtgtt gacgaacacc atgcctgctt caatgccacg
    13261 cgccaggcgt aacactttgc tgacatcctg tgtccagatg tacgacgcga ggccgtactc
    13321 cacgtcgttt gccaggcgta agccttcggc ttcgtcttta aacggcagca ggcaggcgac
    13381 cggcccgaaa atctcttcct gggcaacgcg catacggtta tctacgtccg ccagcaccgt
    13441 tgggcgcagg aagttgccgc ctttcaggtg cgcaggcagg tcggacggtt tatccgggcc
    13501 gcccgccaac agggttgcgc cttcttcaat gccgagacgg atatagccgg agactttttc
    13561 ccagtgttgc tgactgataa gcgccctaac ctgggtattc ggatcgttcg gatcgcccac
    13621 gcgcagacgg ttggcgcgtt cggcaaagcg tttaacgaat tctggataga tgctctgctg
    13681 aataaagatg cgcgaaccgg cggtgcagcg ttcgccgttg attgagaaga tggtgaacag
    13741 ggcggcgtcc agcgcacgct caatatcggc atcttcaaaa atcagcaccg gtgatttacc
    13801 gcccagctcc atggagtatt ttttcagccc ggcgtttttc atgatgttgc gcccggtggc
    13861 ggtaccgccg gtgaacgaca cggcacgcac gtcatgatga cggaccagcg catcgcctgc
    13921 ggttgcgccg tagccctgta ccacgtttag cacgcccgcc ggaataccag cttccagcgc
    13981 cagctcaccc aggcggtcgg cggtcagcgg ggagagttcc gacatcttca gcaccgcggt
    14041 attgcccagc gccagacacg gcgcgacctt ccaggtggcg gtcataaacg gcacgttcca
    14101 cggcgacgcc agcgcacaaa cgcctaccgg ttgcaccagc gtgtagttga gcatcttgtc
    14161 gtcaaccgga taggtcttgc cgttcatctg ctggcagact tccgcgaaaa attcaaagtt
    14221 gtgagaagcg cgtgggatca acacattttt ggtctgatgg atcggcaggc ccgtgtccgc
    14281 ggtttccatc gcggcgatct caggcacgtt ctggtcgatc agatcgccca gacggcgcat
    14341 caggcgcgca cgctctttca tcggcagatc agcccatttc gggaacgcct ctttcgctgc
    14401 cgctaccgcc tgattgatct ccgcttcacc gccagaggcc acatccgcca gcacttcacc
    14461 cgttgccgga ttggtagtct ggaagtagtc gttacctgca acatttttgc cgttgatcca
    14521 atgatttact tttttcattt cgctgtttcc tcactcacaa ttcggttcac caggcggccc
    14581 acgccttcta cttccaccac tacttcatcg ccaggcacca cgtcagataa gccttttggt
    14641 gtgccggtgg cgatcatgtc gcccggattc agggtcataa attcgcttaa gtaggcgatc
    14701 aggaagggca cgctgaagat cagatcggcg gtggtgcctt gctggcgtaa ctcgccgttg
    14761 acgaaggtgc gaagggtcag attatgcggg tccgggatcg cctctttcgg cacgatggtt
    14821 gaaagcatcg gcgtcagtcc gtcgcggctt ttgacccgca ggttagggcg gtagtagttt
    14881 tccagatagt cgcgaatggc gtagtcgtta cacacggtgt agcccgcgac ataatccatg
    14941 gcatcggctt cgctgacgtt acgcgcctgc ttgccaataa ctaccaccag ctccgcttcg
    15001 tagtgcatgt attcaatatt gttcgggcgc acggaggtct ggttgtcgcc agtgagggta
    15061 ttcggcgctt tcaggaacac cagcggctct tccggtggct taaattccag ttcgctggcg
    15121 tggtcggcgt agttcaggcc gagggcaaac agcgtaccgt gcgggtgtgg cagcgttggg
    15181 aagctcttgc gcgtggtcac ttcacgttcg tccactaccg gattttccag cggcgggaaa
    15241 ccttctgcga gaacacgcac gcgatcgctt ggctgtattt ccacgcgcgc ctgtggcgtg
    15301 ccgagcagaa tagcatcgcc tggattcagc gtggcaaatt cgctcagggc gctcagcagc
    15361 tgtgcggcgt tacgttgtaa atcggcggta ttccagtgat cggcaggacg cccgttgatc
    15421 tcggtataga tagtcagatt atcgacattg ctgagagcca cggtttcgcc aatggggcag
    15481 aatccatcac gacattttgc tttgattgcc ggacggtaaa agctctcttc cggcaggctg
    15541 acgtcgttat ccagcgcata tccggcgatg tactctgccg catcttcttc acgtactttc
    15601 gtcgccgttt tccccacaat cagcgcaaca gtcgcaccgc tcagtacttt ttcaccctgt
    15661 ggaaagggaa tcggttcacc gcaaccaatc accgtattgc gcggtttaat aaaccagacc
    15721 gcagttttag gcggggcttt gtagggagat tgctggaacg cttcctgcca ggcatcaagt
    15781 tggctgcgat ggttcaacgc tacggcgaag atagtgcctt tcattaccac tcctcggatt
    15841 cgatcatctt aatcattaat atgttaatga ttacttttat tcccgattgc gtagtttggc
    15901 aaaa
//
LOCUS       DAZPCS010000085        15735 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZPCS010000085 DAZPCS010000000
VERSION     DAZPCS010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15735
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.085"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            114..656
                     /gene="zapC"
                     /locus_tag="ACHUQ7_002568"
     CDS             114..656
                     /gene="zapC"
                     /locus_tag="ACHUQ7_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFR1135925.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(653..1762)
                     /gene="ycbX"
                     /locus_tag="ACHUQ7_002569"
     CDS             complement(653..1762)
                     /gene="ycbX"
                     /locus_tag="ACHUQ7_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFR1135926.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            2006..4114
                     /gene="rlmKL"
                     /locus_tag="ACHUQ7_002570"
     CDS             2006..4114
                     /gene="rlmKL"
                     /locus_tag="ACHUQ7_002570"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFR1135927.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERHKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            4126..6033
                     /locus_tag="ACHUQ7_002571"
     CDS             4126..6033
                     /locus_tag="ACHUQ7_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR1135928.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDLKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVNGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRAFSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            6163..7416
                     /gene="pqiA"
                     /locus_tag="ACHUQ7_002572"
     CDS             6163..7416
                     /gene="pqiA"
                     /locus_tag="ACHUQ7_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFR1135929.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            7421..9061
                     /gene="pqiB"
                     /locus_tag="ACHUQ7_002573"
     CDS             7421..9061
                     /gene="pqiB"
                     /locus_tag="ACHUQ7_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFR1135930.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            9058..9621
                     /gene="pqiC"
                     /locus_tag="ACHUQ7_002574"
     CDS             9058..9621
                     /gene="pqiC"
                     /locus_tag="ACHUQ7_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFR1135931.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            9877..10044
                     /gene="rmf"
                     /locus_tag="ACHUQ7_002575"
     CDS             9877..10044
                     /gene="rmf"
                     /locus_tag="ACHUQ7_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFR1135932.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLSQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(10114..10632)
                     /gene="fabA"
                     /locus_tag="ACHUQ7_002576"
     CDS             complement(10114..10632)
                     /gene="fabA"
                     /locus_tag="ACHUQ7_002576"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFR1135933.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMIDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(10701..12461)
                     /locus_tag="ACHUQ7_002577"
     CDS             complement(10701..12461)
                     /locus_tag="ACHUQ7_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR1135934.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            12646..13098
                     /gene="matP"
                     /locus_tag="ACHUQ7_002578"
     CDS             12646..13098
                     /gene="matP"
                     /locus_tag="ACHUQ7_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFR1135935.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(13174..14220)
                     /gene="ompA"
                     /locus_tag="ACHUQ7_002579"
     CDS             complement(13174..14220)
                     /gene="ompA"
                     /locus_tag="ACHUQ7_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFR1135936.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(14576..15085)
                     /gene="sulA"
                     /locus_tag="ACHUQ7_002580"
     CDS             complement(14576..15085)
                     /gene="sulA"
                     /locus_tag="ACHUQ7_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFR1135937.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            15304..15714
                     /gene="sxy"
                     /locus_tag="ACHUQ7_002581"
                     /pseudo
     CDS             15304..15714
                     /gene="sxy"
                     /locus_tag="ACHUQ7_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
BASE COUNT         3963 a         3775 c         4154 g         3843 t
ORIGIN      
        1 ttatattcat cgttgttgct tatttagaca ttttgtactt ttattgttga ggttattaag
       61 cgaagcgaca atggattgtg ttgctgcggt tttatagggt aggggagagg cagatgcgaa
      121 ttaaaccaga cgataactgg cgttggtatt acgatgaaga gcacgatcga atgatgctcg
      181 atttagccaa tggtatgcta tttcgctcac gttttgcgcg caaaatgttg actccagatg
      241 ccttttctcc cgcaggcttt tgtgttgatg atgccgcgct ctatttctct tttgaagaaa
      301 agtgccgcga ttttaattta tctaaagacc aaaaagccga actggtgctg aatgcactgg
      361 tagcaattcg ttatctcaaa ccgcaaatgc cgaaaagctg gcattttgtc tcccatggtg
      421 aaatgtgggt gcccatgccg ggcgatgcgg cctgcgtttg gttgagcgat actcacgagc
      481 aggtcaattt gctggtcgtt gaatctggcg aaaatgccgc gctatgcctg ctggcacaac
      541 cctgcgttgt tattgcgggt cgcgcgatgc agttgggcga cgccattaaa atcatgaacg
      601 acaggctgaa accgcaggtg aatgttgaca gcttcagcct cgaacaggca gtctaacgcg
      661 tcagcttaag ggcagtcttc ggtacacagc tacagcaaag aatggtgcca tcatcgccca
      721 ttgctgattt tttcagcggt gtgacttcgc cttctaaaag ctgaacacgg caacttccac
      781 aaatgcccgc gcggcaagaa taagggatac gaattccctg attttctaat tgctccagca
      841 acacttgttg gttatttcca cgaaatgcct gtccctgcca gtcaatatct acatttgcgt
      901 ccggttgttg cgtgatgttg gcggtatcat cagcggcagc tgcgccgtaa atttttgccg
      961 gagccgttgc cagaatttcc acctcatcgc caacgcgaat cacgccgcta ttacgggcaa
     1021 ttaaattctg accaaaatcg acatcgccgt tatcctgggc agtgcggaaa gattgcaatg
     1081 tttttaatgg ttcgcctgcc ggatgttttt gccctttttc tgggctgacg gtggtgaaaa
     1141 tacagcggct acaaggttta accacatcaa acaccacatc accaatgcga atcactttcc
     1201 agctatcttc ttcccaggct gacgcgccgg aaaccaccag attggggcgg aattgctcca
     1261 tttttacact ggccggacaa cgttgttgga gatcacgtaa cgaggcttcg ttagcaagaa
     1321 ggtaaggata gccatcagca aatgacagag gtacagtgtt gtggcgtttc acgcgccggg
     1381 tcatttgtgg ccccacccag cgtaattgca cttcgcggga gaaaaatcca cttagccatt
     1441 tgttgatcgc gtctggcgca attcgcgcgg taaaatgtgt gccccaaact tcggttggtg
     1501 cgtcttgtgt ggcgaaatca gcaaaacgaa catatgcgct actgccatct ggtgcggtga
     1561 gatgcaagcc atcatgcaca ggtgaaggag taaaccgtac catctgggga aactggcgag
     1621 cggtaataaa cgtaccgtca ggttccgtga tcataaagat gcgatcgaag gccagaccac
     1681 tgacatcagc cagagcatgt gtaagaccaa tgccgcgcat cgatttaaca ggatgaataa
     1741 aaagccgggt taatgtcgcc acggtgcggt cctcaaatga aaataagccc tcaactttat
     1801 gacatgacgc gcttattagc tataatgcgc aacaattttc ttagcgcctg tcccgttagg
     1861 ctattttatt tgtcagtttg gccctgggtt gtgctcgaaa tcctcacgta cttgtgtacg
     1921 ctccggtttc tccgcgctct ccatgtccag actgactgca acaattacgc ctactgcggt
     1981 aggctcttag agtaaaagtg acgatatgaa ttctctgttt gccagtacgg cccgtgggct
     2041 ggaagagctg ttaaaaactg aactggaaaa tctgggggcc gttgaatgcc aggtggttca
     2101 gggtggggtc catttcaagg gcgacacacg gcttgtttac cagagcctga tgtggagccg
     2161 cctggcctcg cgtattatgt tgccgctggg cgagtgtaag gtttacagcg atttagacct
     2221 ctatctcggt gttcaggcga tcaactggac agagatgttt aatcctggcg cgaccttcgc
     2281 tgtccacttc agtggtttga atgacaccat ccgcaacagt cagtacggcg cgatgaaagt
     2341 gaaagacgcg atcgtcgatg ctttcacacg gaaaaatctg ccgcgtccaa atgttgatcg
     2401 cgatgcgccg gatatccgcg ttaacgtctg gctgcataaa gaaaccgcca gtatcgctct
     2461 tgatctgagt ggtgatggtt tacatctgcg tggctatcgc gatcgtgctg gtattgcgcc
     2521 gatcaaagaa accctggcag ccgctattgt gatgcgatcc ggctggcagt caggaacacc
     2581 gctgctcgat ccgatgtgtg gttccggtac gttgctgatt gaagcggcga tgctggcgac
     2641 cgatcgcgca ccaggcttgc accgtgggcg ttggggcttt agcggctggg cgcagcatga
     2701 tgaagctatc tggcaggaag tgaaagcgga agcgcaaact cgcgcccgta aaggcctagc
     2761 tgagtatagc tctcattttt acggttcgga cagcgacgca cgggtgattc aacgtgcgcg
     2821 cactaacgcc cgtcttgcgg ggattggtga actgatcacc tttgaggtga aagatgtcgc
     2881 gcaactgacc aatccgctgc cgaaagggcc gtacggtaca gtgttgagca acccgccata
     2941 cggtgaacgt ctggacagcg aaccggcgct gattgcgctg catagtctgc tgggccgaat
     3001 catgaaaaac cagttcggtg gctggaatct ctctttgttt agtgcctcgc cggatctgct
     3061 aagctgcctg cagctgcgtg cagacaaaca gtacaaggcg aaaaacggcc cgctggactg
     3121 cgtacagaaa aattaccacg ttgccgaaag cacaccagac agtaaaccgg caatggtagc
     3181 ggaagattac gccaaccgcc tgcgtaagaa cctcaaaaaa ttcgagaagt gggcacgcca
     3241 ggaagggatt gaatgttacc gcctgtatga cgccgatctg ccggaatata acgttgccgt
     3301 tgaccgttat gccgactggg tggtggtgca ggagtatgcg ccgccaaaaa ctattgatgc
     3361 gcacaaagcg cgtcagcgtc tgttcgatat tatcgctgca accatttcgg tactggggat
     3421 tgcgccaaac aaactggtgc tgaaaacccg tgaacgccat aagggcaaaa accagtacca
     3481 gaaactgggc gagaagggcg agtttcttga agttaccgaa tataacgctc acttgtgggt
     3541 gaacctgacg gattatctcg atactggtct gttcctcgat caccgtatcg cccgccgtat
     3601 gctcggtcag atgagcaaag gcaaagattt cctcaacctg ttttcttata ccggcagcgc
     3661 caccgtgcat gcgggattag gcggtgcacg cagcaccacc accgtggata tgtcgcgtac
     3721 ttatctggaa tgggcagaac gcaacctgcg tctgaatggc ctgactaggc gtgcgcatcg
     3781 cctgattcag gccgattgcc tggcgtggct gcgtgaggca aatgaacagt tcgatctgat
     3841 ctttatcgat ccgccaacct tctctaactc aaaacgaatg gaagatgcgt ttgatgttca
     3901 gcgcgatcat ctggcgctga tgaaagattt gaaacgtctg ctgcgtgcag gtgggacgat
     3961 catgttctcg aacaacaaac gtggcttccg tatggatctc gacggcctgg caaaactggg
     4021 actgaaagca caagaaatta cgcaaaaaac gctctcccag gatttcgccc gtaaccgcca
     4081 gatccacaac tgctggctga ttaccgcagc ctgaaaggaa tagtaatgtc attaatcagt
     4141 atgcatggcg catggctgtc gttcagcgac gcgccgcttc tcgataacgc agaactgcat
     4201 atcgaagata acgaacgtgt ttgtctggtg ggccgcaacg gcgcaggaaa atcgacgtta
     4261 atgaaaatcc tcaaccgtga acaagggctg gatgacggtc gcattattta tgagcaagat
     4321 ttgattgtag ctcgtctgca acaggatccg ccgcgtaacg ttgagggtag cgtttatgat
     4381 ttcgttgccg aaggcattga agaacaagcg gaatatctga aacgctatca cgatatttcg
     4441 cgcctggtga tgaacgaccc gagcgagaaa aatctcaacg aactggcgaa ggttcaggaa
     4501 cagctggatc accacaacct gtggcagctg gaaaaccgca tcaacgaagt gctggcgcaa
     4561 ctggggttag atcctaacgt tgcgctgtcg tcgctttccg gcggctggtt gcgtaaagcg
     4621 gcattaggac gcgcgctggt gagcaatccg cgcgtattgc tgctggacga accgactaac
     4681 catctggata tcgaaaccat cgactggctg gaggggttcc ttaaaacctt caatgggacg
     4741 attattttca tctcccacga ccgttcgttt atccgcaata tggcgacgcg cattgttgat
     4801 ctcgatcgcg gcaagctggt gacctatcca gggaattacg accagtacct gcaggaaaaa
     4861 gaagaagccc tgcgcgtgga agaactacaa aatgccgagt tcgatctcaa actggcgcag
     4921 gaagaagtgt ggattcgcca ggggatcaaa gcacgccgta cccgtaatga aggccgcgta
     4981 cgcgccctga aagcgatgcg tcgcgaacgt ggtgaacgtc gcgaagtgat gggtaccgca
     5041 aagatgcagg tggaagaggc cagccgctcc ggtaagatcg ttttcgaaat ggaagacgtt
     5101 tgctaccagg ttaacggtaa gcaactggtg aaagattttt ctgcccaggt tctacgtggc
     5161 gacaaaattg ccctgattgg tccgaatggg tgcggcaaaa ccacgctgct gaaactgatg
     5221 ctcggtcagc ttcaagcgga cagcgggcgt attcacgttg gcaccaaact ggaagtggct
     5281 tatttcgatc agcaccgcgc ggaactggat cccgataaaa cggtgatgga taaccttgcc
     5341 gaaggtaagc aagaggtgat ggttaatggc aagccacgcc acgtattggg ctatttgcag
     5401 gactttctgt tccatccgaa acgggcgatg acgccagtac gtgcgttttc tggcggtgag
     5461 cggaaccgct tgctgctggc gcgtttgttc ctcaaaccaa gcaacttatt gattcttgac
     5521 gaaccgacca acgatcttga tgtcgaaacg ctggaactgc tggaagaact gatcgacagc
     5581 tatcagggca cggtattgct ggttagccac gatcgtcagt ttgtcgataa caccgttaca
     5641 gaatgctgga tcttcgaagg cggcggtaaa attggtcgtt atgttggcgg ttatcatgat
     5701 gcccgtggtc agcaagaaca atatgtggca ctcaaacagc ctgcggtgaa aaaaagcgaa
     5761 gaagccgccg cgccaaaagc agaaactgta aaacgcagca gtagcaaact aagctataaa
     5821 ttgcagcgcg aactggagca gctaccgcaa ttgctcgaag atctggaggc gaagctggaa
     5881 gccctacaga cgcaagtggc ggatgcttcc ttcttcagtc agccgcatga gcagacgcaa
     5941 aaagtgcttg ctgatatggc tgctgcagag caggagctgg agcaagcctt tgaacgctgg
     6001 gagtatcttg aagcgttaaa aaatggtggc tgatcgcaaa atagtcgatt taaacagaga
     6061 cagctgacaa cggtaaaatt gcctgatgaa ctgggtttat caggcatgaa aaccaacact
     6121 ttgttagtca tctgtaaaat agcgcatcat taaggagtac caatgtgcga acatcatcat
     6181 gccgcgaagc acatcctgtg ctcgcagtgt gacatgctgg tggcgttacc gcgccttgag
     6241 catggtcaga aagcggcatg tccccggtgt ggcacaacgt taaccgtggc gtgggatgcc
     6301 cctcggcagc gtcccaccgc ctatgcgttg gctgcactgt tcatgctgtt gctgtccaac
     6361 ttgtttcctt ttgtgaatat gaacgttgca ggagttacca gtgaaattac attactggaa
     6421 attcccggcg tgcttttttc tgaggactac gccagcctcg gcaccttttt cctgttgttt
     6481 gtgcaactgg ttcccgcgtt ttgtctgata accattctgt tactggtgaa tcgcgcggaa
     6541 ttaccggtcc gtttaaaaga gcaactggca cgggtgcttt ttcaactcaa aacctgggga
     6601 atggcggaga ttttcctcgc gggtgtgctg gtcagtttcg ttaaactgat ggcttacggc
     6661 agcattgggg taggcagcag ctttctcccc tggtgtttat tttgtgtcct gcaactgcgc
     6721 gcttttcagt gcgttgatcg tcgctggtta tgggacgaca tcgccccgat gccagaactg
     6781 cgccagccgc taaaaccagg cgtcacgggg atacgtcagg ggctgcgttc gtgctcctgt
     6841 tgtacggcaa tccttcctgc tgatgaaccc gtgtgcccgc gttgtagtac caaagggtac
     6901 gttcggcgta gaaacagcct gcagtggaca ctcgcgctgc ttgtaacgtc catcatgctg
     6961 tatcttccgg ctaatatttt gcccatcatg gtgacggatt tattaggctc gaagatgccg
     7021 tcgacgattc tcgctggggt cattctgtta tggagcgaag gctcttatcc cgtcgctgcg
     7081 gtgatctttc tggccagtat tatggtgcca acgttaaaga tgatcgccat cgcgtggctg
     7141 tgttgggatg ccaaagggca tggcaagcgc gacagtgaaa gaatgcattt gatttatgaa
     7201 gttgttgagt ttgtaggccg ctggtcgatg attgacgttt tcgttatcgc ggtgctctcg
     7261 gcgctggtgc gtatgggagg tttaatgagt atttatccgg caatgggtgc attaatgttt
     7321 gctttagtcg tcataatgac aatgttttct gctatgacgt ttgacccgcg tttgtcgtgg
     7381 gatcgtcaac ctgaatcaga gcatgaggag tcctgacagt atggaatcta ataatgggga
     7441 agccaaaatc cagaaagtga agaactggtc tcccgtgtgg atatttccta tcgtcacggc
     7501 gctcattggg gcctgggttc ttttttatca ttacagccat cagggaccgg aagtgaccct
     7561 gatcaccgcg aatgcggaag gaattgaagg tggcaaaacc accattaaaa gccgtagcgt
     7621 tgacgtcggc gtggttgaaa gcgccacact ggctgatgat ttgacgcacg ttgaaatcaa
     7681 agcgcggctg aattccggta tggaaaaatt gctgcataaa gacaccgtct tttgggtggt
     7741 gaaaccgcag attggtcgcg aagggattag cggcctggga acgctgctgt ctggagttta
     7801 tatcgaactg cagccaggcg cgaaaggcag caaaatggat aaatacgatt tgctggactc
     7861 accaccgttg gccccgcctg atgcgaaagg tatccgtgtg attctcgata gcaaaaaagc
     7921 cgggcagctc tcgccaggag atccggtgct gttccgtggc tatcgggtag gttcggttga
     7981 aaccagcacc ttcgatacgc aaaaacgcaa tatcagttat caactgttca tcaatgcacc
     8041 ttatgaccga ctggtgacca gcaatgttcg cttctggaaa gatagtggca ttgcggttga
     8101 tctgacgtca gcggggatgc gtgtggagat gggctcattg acaacgctgc ttagtggcgg
     8161 tgttagcttt gatgtgccgg aaggtctgga tttagggcag ccagtggcac cgaaaacagc
     8221 tttcgttttg tatgatgatc agaagagcat tcaggattcg ttgtacaccg atcacattga
     8281 ttatctgatg ttctttaaag attcggtacg cggtctgcaa ccgggagctc cggtagaatt
     8341 ccggggtatt cgcctgggta ccgtaagcaa agtgccattc tttgcgccga atatgcgtca
     8401 gacatttaac gatgattacc gtattccggt actgattcgt atcgagccag agcggctgaa
     8461 aatgcagctt ggcgaaaatg cggatgttgt tgagcacctt ggcgaattgt tgaaacgtgg
     8521 tttacgcgga tcgctgaaaa ccggaaacct ggtcactggt gcactgtatg ttgatctcga
     8581 tttctatcca aatacgcctg caataaccgg tattcgtgaa tttaatggtt atcagattat
     8641 cccgactgtt agcggcggct tggcgcaaat ccagcaacga ctgatggaag cgttggataa
     8701 gatcaacaaa ctgccattga atccgatgat tgaacaggca accagtacgc tttctgaaag
     8761 tcagcgcaca atgaaaaacc tgcaaacgac gctggatagc atgaacaaga tcctcgccag
     8821 ccagtcgatg cagcagttgc cgacggatat gcagtcaacg ttgcgtgaat tgaatcgcag
     8881 catgcagggc ttccagcctg gctccgcagc ctacaacaag atggtggcgg atatgcagcg
     8941 ccttgatcag gtgttgcgag aactgcaacc ggtgctgaaa acgctcaatg agaagagtaa
     9001 cgcgctggta tttgaagcga aggacaaaaa agatccagag ccgaagaggg cgaaacaatg
     9061 aaaaagtggc tagtgacgat tgcagcactg tggctggccg gatgcagctc cggcgaaatt
     9121 aataaaaact attaccagtt acctgtggtg cagagcggta cacaaagtac cgccagccag
     9181 ggcaatcgtc tgttatgggt agagcaggtc actgttcctg actatctggc ggggaatggt
     9241 gtggtttatc aaaccagtga tgtgaagtat gtgattgcca acaacaactt gtgggccagc
     9301 ccgttggatc aacagttgcg caacaccctg gttgccaacc tgagtacgca actgcccggc
     9361 tgggtggttg cctcccagcc tctgggaagc gcccaggaca cgctcaatgt taccgtaacg
     9421 gagtttaacg gtcgctatga tggcaaggtc attgtcagtg gtgagtggct gttgaaccac
     9481 cagggacaac tgatcaaacg tccgttccgt ctggaaggag tgcaaactca ggatggttac
     9541 gatgagatgg ttaaagtgct ggccggtgtc tggagtcagg aagccgcttc tattgcacaa
     9601 gagataaagc gtctacctta attataaaga tttgtaaata taaccgtctc cggtatgttg
     9661 cctgaggcgg tttttttgtt tctaacgtgc ggaaaaattt gttcctcttc acattttttg
     9721 tacaaccgac atgcccgtgt agctcacaaa tatgacagtg gcgtgaattt tgcgcattga
     9781 cggcagttat gattcgcggt attgcttaac tgtgattgca catttagtaa tcactgtttt
     9841 cttttccacc agaaaccagt atgagggaaa cgaggcatga agagacaaaa acgagatcgc
     9901 ctggaacggg cacatcaacg tggttatcag gccggcatcg ccggacgctc aaaagaaatg
     9961 tgtccctatc agacgctgag tcaaaggtca caatggctgg gaggctggcg agaagccatg
    10021 gcggacaggg tagtaatggc ctgattctgt ctctttaaaa agaaacctcc gcattgcgga
    10081 ggtttcgcct tttgatactc tgtctgatga taatcagaag gcagacgtat cctggaacag
    10141 accgactttc aggtcgctgg cggtatagat cagacgacaa tcaaccagca cttcgccatc
    10201 tgccagaccc ataatcagac gacgattaac aatgcgttta aagtgaatac ggtaggtcac
    10261 ttttttcgct gtcggcagta cctgaccagt gaatttcact tcgccaacgc ccagcgcgcg
    10321 gcctttacct tcgccgccca gccagccgag gtagaaccct accagctgcc acattgcgtc
    10381 caggcccagg catcccggca taaccggatc gccaatgaag tggcatccga agaaccacag
    10441 atccggattg atatccagtt ctgcttcaac ataccctttg tcgaagttac cacccgtttc
    10501 ggtcattttg accacacggt ctatcatcag catgttcggt gctggcaatt gcgggccttt
    10561 agcgccaaac agttcaccgt gaccagaggc aagaaggtct tcttttgtat aggattcgcg
    10621 tttatctacc atgttctctg taagccttat tttattgaag cacgcaggat agctaacacg
    10681 tgtacgctga acaagtccga tcagttcgga ataaaccagt tcagccaacg taatggccat
    10741 ggaaaacggt gacgtccttc ctgttgcgat gcttgcgcga tacgttcctg gatggtttgc
    10801 atcagcgtcg tttggccttc gccatcccac accagattta ataataacgg cagtgcgtca
    10861 gtcacatcgt ctactgccca gatggtgaat ttgccttctt ctaccgcttt caccagttca
    10921 ctgtgaagac ttaaatggcg aacgttagca gtggggataa tgacaccttg tttccccgtt
    10981 aactcacgtt gctggcaaat agcaaagaag ccttcgattt tctcatttaa accaccgacc
    11041 ggctggacgc ggccgaactg atcgactgaa cctgtgatag cgatactctg attcactggc
    11101 acatcagcga gggcgcttat cagggcgcag agttcagcca tcgaggcact atcgccatcc
    11161 acttcactgt atgactgctc aaatgtcagc gatgctgaga aggggatctg ttgctcaagc
    11221 tgtagttccg acatcaggaa cgcctgcatg atcatcatcc ctttcgcatg gatattgccg
    11281 ccaagctccg ctttgcgttc gatgtcggtg aattcaccat cgccaatatg cacaacgcag
    11341 ctaatgcgag aaggttcgcc aaaagcgcgt ggatgacccg gaaattcaat gaccgaaagg
    11401 gcgttaattt gcccgatgcg ttcgccttcg gtttcaatca ggatttgctc ctgaaggatc
    11461 tcatcctgca tacgttcagc gaggaaacct tcgcgccatt cacgctgctg cagcattaag
    11521 tttagctgct cgccggagaa ggtgtcgcca tcacacaggg aggcgacctc tttacactgg
    11581 cggaggatcc actgcgggct aagcggaagt gtttcttgtt caccggtgta gcgtgctgct
    11641 tcgcggataa gtaccggcca agcatccgct cccggggcag gtaagtgatt atgtctggcg
    11701 gtaaatgtca cccagcgaca ccactggctt actgactccg catcgacaat ctgcagagta
    11761 tcttcaaatt cgctataaat agcctgctct gaaagctctg gctccatctc ctggaaatca
    11821 gccaatgatt cgcgttcgcc taccagaatg actttcagct tcaatggcat tgaaggcaca
    11881 gagacgggga gagggcgcga ctcatcaaac gcaacccagt caaaacgctc gcggttaacg
    11941 atatttttca gccgcatcca cagcagaggt tgcgccagca gtgtacgcaa agagatgatg
    12001 agaataccgc catttgcctg atgcaccaga ccaggctgca gggtaatgtc gccattaaac
    12061 tggcgcaggc agccaaagag ttgctccgct tctacccagt cggcagcgac aacttgcgtt
    12121 aaagtcgcaa aattatcatc tgcactcact gcgtgacgta agcggatgga gtggccggaa
    12181 acctcatagt gatcgcccac cagttggcct gcatcgcttt gtagcgtacg cgcggcatcg
    12241 gcaataagat tcagatactc agactcttcc ggggccttag ccagcataaa agaggaggat
    12301 gctcgcgtat gcagcaactg ctccagcgca aattgcagtc gcggttgagt atcactgaat
    12361 aaaggatcgt tttcgtcaat caaatgtggc tgagcaaata tttcctgata gctatcggta
    12421 tcaggaacca ggtcacgcca tgcaagtttc gtaatggtca aagttgatgt tttttagtct
    12481 gttgtcaaag ccgcgattat accgtaaccg gcactacagc acacgtagaa agcaccgaca
    12541 atactcctgg ctgggcgtta aagctcacag gatggagatt cttttcttca ctggcctaaa
    12601 aagctgatat tctgtaaaga gttacacggt aacattgaga tcgctatgaa atatcaacaa
    12661 cttgaaaatc ttgaaagcgg ttggaaatgg aagtatctgg tgaagaagca tcgcgaaggg
    12721 gagttaatta cccgttacat agaagccagt gccgcccagg aagccgtcga tgtgttgctc
    12781 tcgctcgaaa atgaacccgt gctggtaaat ggctggattg acaagcatat gaatccggaa
    12841 ctggtcaatc gcatgaagca gaccattcgg gccagacgga agcgccattt taatgcggaa
    12901 caccagcata cgcgcaaaaa atcgatcgat ctggaattta ttgtctggca acgtctggct
    12961 ggtcttgcac agcgtcgcgg taaaacgctt tctgaaacga ttgttcagct gattgaagat
    13021 gcggaaaaca aagagaaata cgcgaataaa atgtcttctc ttaagcagga tctgcaggca
    13081 ttgctgggta aggaataact gacgaaagtc agttcaattt actaaaggca aaaaaaaccc
    13141 cgcagcagcg gggtttttct accagacgat aacttaagcc tgcggctgag ttacaacgtc
    13201 tttgatacct ttaacttcga tctctacgcg acgatccgga gccaagcagt cgatcagtgc
    13261 agcacgctgt ttcacgttgt cacaggtgtt gccagtaacc gggttggatt cgcccatacc
    13321 acgtgcggag atcttgtctg ccgggatacc tttggagatc aggtaatcaa caacagactg
    13381 agcacggcgc tcggacagac cctggttgta agcgtcagaa ccgatgcggt cagtgtaacc
    13441 cagaacaact acggaaccgt ctttcggatc taggttgctc agctggctgt acagctgatc
    13501 cagagcagcc tgaccttccg gtttcagggt tgctttgttg aagttgaaca gaacgtcaga
    13561 cttcagagtg aagtgcttgg tctgtacttc cggtgccgga gctggagcaa ctaccggagc
    13621 tgcttcgccc tgaccgaaac ggtaggaaac acccaggctc agcagaccgt tgtccggacg
    13681 agtaccgatg gtgtttgcgt caccgatgtt gttggtccac tggtattcca gacgggtagc
    13741 gatttcagga gtgatcgcgt actcaacacc acctgcgaaa accggagaaa cgccagtgtc
    13801 gtggtcttta aaggatgcgc caccaggtac gttagccttg gtgtctgcac gccataccat
    13861 accacccaga cgagtgtaga tgtccagatc gtcagtgatt gggtaaccca gtttagcggt
    13921 cagctgaacg ccctgagctt tgtatgcgcc gttgatgttg tcgcctttgt acggcatacg
    13981 acctaaccag tcgtaaccca tttcaaaacc aacatacggg ttaacctggt aaccaccaaa
    14041 agcacctgca cccagttggt tttcgtgggt cggaccattg ttaggaataa aaccagtgtc
    14101 atggtactgg gaccagccca gtttagcacc agtgtaccag gtgttatctt tcggagcggc
    14161 ctgcgctacg gtagcgaaac cagccagtgc cactgcaatc gcgatagctg tctttttcat
    14221 tttttgcgcc tcgttatcat ccaaaatacg ccatgaatat ctccaacgag ataacacggt
    14281 taaatccttc accgggggat ctgctcaata ttaactctac cgatatcttc ggcttatgcc
    14341 gagcacccct ggcgatgtaa agtctacaac gtagttgaaa acttacaagt gtgaactccg
    14401 tcaggcatat gaaaaaaaag tcttgtataa ggtatgttta atctttttgt cagcgacaat
    14461 ttacagaaaa aaaacgcgga aaccgcttca gacaagcctt cgcaaggaaa attagtcacg
    14521 actgaaagca ttggctgggc gacaaaaaaa gttctaggat taatcctaaa tttacttaat
    14581 gatacaaatt agagtgaatt tttagcccgg aaagttgtct cgttgtgtga gaggatgcgc
    14641 ttaccggacg cataataaac cccatagcgt taccttcatt tgccgcatca acaagttcag
    14701 catgctcttc ttcagtcaaa tcatctgtca accaaccgat caccacactg taattgcccg
    14761 tgcgtaaagc gcgaaccatt gactccactg tgtggcaagg ggagagctgg ctaatctgca
    14821 ttactttcgt taagggtagc ccagatgcct gaacccattc ccgactcagt ttttgttgcg
    14881 gtgttaacca gagttgccag cgcgattgct gaccgagttg ctgtaacaat ggcaacagta
    14941 gaagttgcgt catcatgggc tgatcttcgc gatagacaac ttcactgata agcccggctg
    15001 tagtgttttc cgtagagaca cgcgcaattt tacttgctgc ggatgagaac gacgaagaac
    15061 gatgtgcata gcctgaagtg tacataatca atccagcccc tgtgagttac tgtatggatg
    15121 tgcagtacat ccagtgacaa caaagatcaa ccctattttc ggaaagagcc tcgcaaattt
    15181 tgtcgttggt gacgggaaaa cataaattaa tcttgcccct taagaataag ttgcctattt
    15241 tcgtagttaa cggatccgtt aatgtgaatc attcttttat gttatgattt taaaaggaat
    15301 tttatgaaaa gcctctccta taagcggatc tataaatcac aagaatacct ggcaacgttg
    15361 ggcacaattg aataccgatc attgtttggc agttacagcc tgaccgttga cgacacggtg
    15421 tttgcgatgg tttctgatgg tgagttgtat cttcgggctt gtgagcaaag tgcacagtac
    15481 tgtgtaaaac atccgcctgt ctggctgaca tataaaaagt gtggccgatc cgttaccctc
    15541 aattactatc gggttgatga aagtctatgg cgaaatcaac tgaagctggt gcgtctgtcg
    15601 aaatattctc tcgatgcagc gctgaaagag aaaagcacgc gcaatacccg ggaaagactg
    15661 aaagatttgc ccaatatgtc ttttcatctg gaagcgattc tcggggaggt gggtagactg
    15721 gccccctgaa tctcc
//
LOCUS       DAZPCS010000086        15412 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZPCS010000086 DAZPCS010000000
VERSION     DAZPCS010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15412
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.086"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..1177)
                     /locus_tag="ACHUQ7_002582"
     CDS             complement(<1..1177)
                     /locus_tag="ACHUQ7_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1135938.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLADARARRDEARKLLANGVDPSENKKAVKVEQEQEAITFEVV
                     ARDWHASNQKWSASHSARVLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLE
                     VAARLQQRTTAIMRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRID
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEIDFETAMWTIPGEREQLEGVKHSQRG
                     SKMRTPHLVPLSRQALSILEKIKSMSGNRELIFVGDHDPRKPMSENTVNKALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSRDAVERQMSHQERSSVRAAYIHKAEHLGERRLM
                     "
     gene            complement(1376..1460)
                     /locus_tag="ACHUQ7_002583"
     tRNA            complement(1376..1460)
                     /locus_tag="ACHUQ7_002583"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1424..1426),aa:Leu,seq:caa)
     gene            1656..2675
                     /gene="ahr"
                     /locus_tag="ACHUQ7_002584"
     CDS             1656..2675
                     /gene="ahr"
                     /locus_tag="ACHUQ7_002584"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFR1135939.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2804..4306
                     /locus_tag="ACHUQ7_002585"
     CDS             2804..4306
                     /locus_tag="ACHUQ7_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFR1135940.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVFGQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(4425..5507)
                     /gene="lptG"
                     /locus_tag="ACHUQ7_002586"
     CDS             complement(4425..5507)
                     /gene="lptG"
                     /locus_tag="ACHUQ7_002586"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFR1135941.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEVLGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5507..6607)
                     /gene="lptF"
                     /locus_tag="ACHUQ7_002587"
     CDS             complement(5507..6607)
                     /gene="lptF"
                     /locus_tag="ACHUQ7_002587"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFR1135942.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(6670..6720)
                     /gene="ytgA"
                     /locus_tag="ACHUQ7_002588"
     CDS             complement(6670..6720)
                     /gene="ytgA"
                     /locus_tag="ACHUQ7_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFR1135943.1"
                     /translation="MSIIILATISTTQENK"
     gene            6874..8385
                     /gene="pepA"
                     /locus_tag="ACHUQ7_002589"
     CDS             6874..8385
                     /gene="pepA"
                     /locus_tag="ACHUQ7_002589"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFR1135944.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGQAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMTNHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8519..8962
                     /gene="holC"
                     /locus_tag="ACHUQ7_002590"
     CDS             8519..8962
                     /gene="holC"
                     /locus_tag="ACHUQ7_002590"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFR1135945.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGSAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            8962..11817
                     /gene="valS"
                     /locus_tag="ACHUQ7_002591"
     CDS             8962..11817
                     /gene="valS"
                     /locus_tag="ACHUQ7_002591"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFR1135946.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11871..13067)
                     /locus_tag="ACHUQ7_002592"
     CDS             complement(11871..13067)
                     /locus_tag="ACHUQ7_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HFR1135947.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILAFLALLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVM
                     PSLPFL"
     gene            13260..13763
                     /locus_tag="ACHUQ7_002593"
     CDS             13260..13763
                     /locus_tag="ACHUQ7_002593"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR1135948.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(13809..14225)
                     /gene="rraB"
                     /locus_tag="ACHUQ7_002594"
     CDS             complement(13809..14225)
                     /gene="rraB"
                     /locus_tag="ACHUQ7_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFR1135949.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            14387..15400
                     /gene="argF"
                     /locus_tag="ACHUQ7_002595"
     CDS             14387..15400
                     /gene="argF"
                     /locus_tag="ACHUQ7_002595"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFR1135950.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
BASE COUNT         3978 a         3933 c         3968 g         3533 t
ORIGIN      
        1 acatcaacct acgctcccca agatgttccg ccttatggat gtaagctgct cgcacagagc
       61 tacgctcctg gtgactcatc tgccgctcta ccgcatccct cgaccataat cccgactcaa
      121 tcaacgaact acaagccatc gtcctgaagc catgcccaca aacctcgacc ttcgtgtcat
      181 accccattac gcgcaaagcc ttgttcaccg tattctcact catcggctta cgtggatcgt
      241 gatcgcccac aaaaatcagc tctcgatttc cgctcatgct tttgatcttt tccaaaatac
      301 ttagggcctg ccgcgacaaa ggtacaaggt gtggtgtccg catcttcgag ccacgctgag
      361 aatgcttgac tccttccagt tgctcacgct cacctgggat tgtccacata gccgtttcaa
      421 aatctatttc tgaccagcgg gcaaaacgca gctcgctcga acggatgaaa accaataaag
      481 tgagttcaac cgccagtcga gttaacggtc tgccagaata gtgatctatg cgatgaagta
      541 attcaggtat acggttcagt tcaagagctg cacggtgctg tcttttcgcc gtagcaaccg
      601 cacctgcaat ctcttgcgcg gggttgtagt cgattaagcc gctctgaacg gcaaagcgca
      661 taatcgcggt agtacgctgc tgtaaacggg cggcaacttc gagccgcccg gatgactcga
      721 ctgctttaat gggtacaaga agatcccgcg tcttcagttc cgcaatgttc cgcttaccga
      781 tagcagtaaa gagattatcc tccagacttt tcaaaacacg agcgctatgc gatgccgacc
      841 acttctgatt gctggcgtgc cagtctctgg cgactacttc aaacgttatc gcctcttgtt
      901 cctgttctac cttaacagct ttcttgtttt cactgggatc gacaccatta gctaaaagct
      961 tacgagcctc atcccggcgt gccctggcat ccgccaacga cacttcgggg tatttcccca
     1021 atgccagcat cttctctttg ccgccaaagc gataacgtag ccgccagtat ttggagccgt
     1081 tagggtgaac cagcaaaacc atgccttcac cgtcggtcag cttataggct tttgcttcag
     1141 gcttagccga acgaaccttc acatcactca gagccatgat gagtatcctt tcaagggttc
     1201 tgtgtgggta caaacattat cgaaccggga aatacccgca gttgtacccg catcagtaag
     1261 ttgatgtaga ttgaatcagg ttgacttagg ttgagtgaaa aagcgaggaa agccttgcgg
     1321 atactggatt ttaggcacaa aaaaagacgt ccgttgacgt ctattgatgt tccgatggtg
     1381 ccgaaggccg gactcgaacc ggcacgtatt tctacggttg attttgaatc aactgcgtct
     1441 accgatttcg ccacttcggc actgaagagg tatgcggaaa acgttgtgga ttatacctgt
     1501 cgcacgccac catgcaagcg ccagcttgca cccacctcgc taagtgctga aattttcagc
     1561 attacactca gcgatatgta acctttgtca cactccaggc accccgccct gccatgctct
     1621 acacttccca aacaacacca gagaaggccc caaaaatgtc gatgataaaa agctatgccg
     1681 caaaagaagc gggcggcgaa ctggaagttt atgagtacga tcccggtgag ctgaagccac
     1741 aagatgttga agtgcaggtg gattactgcg ggatttgtca ttccgatctg tcgatgatcg
     1801 ataacgaatg gggattttca caatatccgc tggttgccgg gcatgaggtg attgggcgcg
     1861 tagtggcact cgggagcgcc gcgcaggata aaggtttgca ggtcggtcag cgtgtcggga
     1921 ttggctggac ggcgcgtagc tgtggtcact gcgacgcctg tattagcggt aatcagatca
     1981 actgcgagca aggtgcggtg ccgacaatta tgaatcgcgg tggctttgcc gagaagttgc
     2041 gtgcggactg gcaatgggtg attccactgc cagaaaatat tgatatcgag tccgccgggc
     2101 cgctgttgtg cggcggtatc acggtcttta aaccactgtt gatgcaccat atcactgcta
     2161 ccagccgcgt tggggtaatt ggtattggcg ggctggggca tatcgctata aaacttctgc
     2221 acgcaatggg atgcgaggtg acggccttta gttctaatcc ggcgaaagag caggaagtac
     2281 tggcgatggg tgccgataaa gtggtgaata gccgcgatcc gcaggcactg aaagccctgt
     2341 cggggcagtt tgatctcatt atcaatactg tgaacgtcag cctcgactgg cagccttatt
     2401 ttgaggcgct gacctatggc ggtaatttcc atacggtcgg tgcggttctc acgccgctgt
     2461 ctgttccggc ctttacgtta attgcgggcg accgcagcat ctctggttct gctaccggca
     2521 cgccttatga gctgcgaaag ctgatgcgct ttgccgcccg cagcaaggtt gcgccgacaa
     2581 ccgaactgtt cccgatgtcg aaaattaacg acgccattca gcatgtacgc gatggtaaag
     2641 cgcgctaccg agtagtcctg aaagccgact tctgatccct ttgccgggtg ctgtcgcccg
     2701 gcacttaaat ctctttggaa ggcgtctcgc aatctgctga atttagcggt gaagagtgca
     2761 cagccgttgc ctatactcca ggcaggaaac tggaggaaat ctcatgagcg aacccctgtt
     2821 aattgcccgc acgccggaca cagaactgtt tttactgccg ggaatggcta accgtcacgg
     2881 gctgattact ggcgcaacgg ggacgggtaa aaccgttacg ctgcaaaaac tggcagagtc
     2941 attgtcggaa atcggcgtgc cggtgtttat ggctgatgtg aaaggcgatc tgaccggtat
     3001 cgcgcaggca ggaacggcgt cggaaaaact gctcacaagg cttaaaaata tcggcgtcaa
     3061 tgactggcaa ccgcatgcca atccggtggt ggtgtgggat atctttggcg agaaaggcca
     3121 cacggtgcgg gcgacggttt cggatctggg gccgctgttg ctggcgcgac tgttgaatct
     3181 caacgatgtg caatctggcg tgctgaatat tatcttccgc attgcggacg atcagggatt
     3241 gttgctgctc gactttaaag atttgcgggc gattacccag tacatcggcg ataacgccaa
     3301 atccttccag aatcagtacg gaaatatcag tagcgcatcg gttggtgcca tccagcgcgg
     3361 attactgtcg ctggaacagc aaggcgcagc acacttcttt ggtgagccga tgctggatat
     3421 caaagactgg atgcgcaccg atgccaacgg taaaggcgtt atcaatatcc tcagcgccga
     3481 gaaactttat cagatgccga aactgtacgc cgccagcctg ctgtggatgc tttcagagtt
     3541 gtatgaacaa ttgccggaag cgggcgatct ggagaaacca aaactggtgt ttttcttcga
     3601 cgaggcacat ctgctgttta acgatgcacc gcaggtactg ctggataaga ttgagcaggt
     3661 gataaggctt attcgctcaa aaggcgtggg cgtctggttc gtttcgcaaa acccgtctga
     3721 tattccggat aatgtgttcg gccagctcgg taatcgtgtt cagcacgctt tgcgggcttt
     3781 tacgccaaaa gatcagaaag cagtgaagac agcggcgcaa accatgcggg ccaatccggc
     3841 atttgatacc gaaaaggcaa tccaggaact ggggaccggc gaggcgttaa tctcgtttct
     3901 cgatgcaaaa ggaagtcctt ctgtggtgga acgggcgatg gtgatcgcac cttgttcgcg
     3961 aatggggccg gtgacggaag atgagcgtaa tggcctgatt aatcactccc cggtgtatgg
     4021 caaatatgaa gatgaggtgg accgcgagtc cgcctatgag atgctgcaaa aaggctttca
     4081 ggccagtacc gagcagcaaa ataatccccc cgtgaaaggg aaagaggtgg cggtggatga
     4141 cggtattctt ggtggattga aggatatttt gtttggcact accggaccac gcggcgggaa
     4201 gaaagatggt gtggtgcaaa caatggcgaa aagcgccgct cgccaggtga cgaatcagat
     4261 tgtacgcggg atgttgggga gtttgctggg ggggagaaaa agataagtca gacgtgatct
     4321 gtgcccctca ccctaaccct ctccccaaag gggcgagggg accgttcgaa ctcgtatttg
     4381 actttgtcaa caataataaa gggagctttc gctcccttgt tagattacga ttttctcatt
     4441 aacagccaca ggctgattaa gaagaagctg gcacttggca acagtgcgcc gatgatcggc
     4501 gggatgccgt aaaccaacgt cagcgggccg aagatctggt ccagtacgta gaagacaaaa
     4561 ccgaagctga tacctgtgac cacacgcacg cccatcggta cgctacgcag tgggccaaag
     4621 ataaacgaca gcgccatcag catcatcacc gccacggata gcggctggaa gattttgctc
     4681 cacatgttga gctgataacg tccggcatcc tgaccgctcg acttcagata cttcacgtag
     4741 ttgtgcaaac cactgatgga gagtgcatcc gggtccagcg ccaccacgcc cagtttgtct
     4801 ggcgtgaggt cggttttcca ggtgccgctc accgtctgcg aaccggtaat ctgtttcgga
     4861 ttggtcagat cagattcatc aacctgcgac agacgccaga ctttatgttc cgggtcaaac
     4921 ttcgcggtag cggcataacg tacggattgc agacgacgat tctcgttaaa ggcataaata
     4981 ctgatgccac ctaacacctc gtcaccttta acccgctcaa tgtagacgaa gttgttgcca
     5041 tctttcgccc ataaaccttg ctgggtagag agcaacgagc cgccgtacat cgcctgcgca
     5101 cggtagttac gcgccatctg ttcgccctgc ggcgcgaccc attcgccaat cgccatcgtc
     5161 agcaagacca gtggaatggc ggttttcatc accgacagcg ctacctgcat acgggtaaaa
     5221 ccagaagcct gcatcaccac cagttcgctg cgctgcgcca gcatcccaag accaagcaac
     5281 gccccaagaa gagccgccat cgggaagaag atctgcacat ctttcggcac gctcagcaag
     5341 gtatacattc ctgcgcctaa cgcgtcgtaa ctcccctgcc cggctttttt cagctgatcg
     5401 acaaatttga taatgcccga cagcgacacc agcatgaaca gcgtcatcat gatggtggtg
     5461 aaaatagttt taccgatata gcggtcaagt acgccaaaag gttgcatcac accgctcctt
     5521 tacgcgaaaa actggcgcgc aggcggcgga ccggcacggt gtcccaaagg ttgagaacaa
     5581 tcgccaaagc cagataaatc aggttaacgg tccacatcca cagtgtcggg tccagcttac
     5641 ctttaccgcc gttcgatttc agggaggtct ggatcaggaa gaaaagtaga tacagcagca
     5701 tggctggcag catcgacagt acgcgtccct gacgcgggtt aaccacgctc aacggtacaa
     5761 ccataagtgc catcataaac acggtgaata ccaacgtgat acgccagttc agttctgcgc
     5821 gagcacgatc ggtgtcagtg ttccacaatg tgcgcatgtc catctggtcg gtatcgttcg
     5881 ggtcgagcgc caccgcctgg tgaccaatga tcgcctgata atcctggaaa tccgtaatgc
     5941 ggaaatcacg taacagtgca gtgccttcga agcgcgttcc ctggttgaga gtgacgacct
     6001 gggagccgtc gcgcagctgg gttaaatgtc cggaatcggc caccaccaca gaaggacgtg
     6061 cattaccttt tggtcgaatt tgtgcgagga acacatcttt gaaatcgctg ccgtcaacgc
     6121 tttcgatgaa cagcaccgag ctgccattag tcgcttgctg gaattgccct tgcgccagcg
     6181 ccgccatgcc agggttcgct ttcgcttctg ctaacacttc atcctgatga cgcgatgacc
     6241 acggtcccgc ccacatcacg ttaaccgccg cgacgattgc cgtgaatacc gcaaggatca
     6301 ttgccgcttt caccagaacc gctttgctca agccgcaggc atgcattacc gtaatttcac
     6361 tttcggtata cagtttgccc agcgtcatca gcagcccgag gaacaggctt aatggcagga
     6421 taagctgcgc catttccggc acgcccaacc cgagaaggga gagcaccaga ttcgccggaa
     6481 tatcgccgtc aaccgctgcg ccgaggatcc tcactaactt ttgacagaag aagatcaaaa
     6541 gcaagatgaa gagtatcgcc agctggcttt tgagcgtctc ccgcaccaga tatcttatga
     6601 ttatcacttt aaatacgccc gtaaaaactc gtcttttgca ggattttagc ttgtttcatg
     6661 gcttaaacgt catttattct cttgagtcgt cgaaatcgtc gctaagataa ttatactcaa
     6721 cggattcacc tctcagattt tgttctgacg tgccaatgcc gtaataacgt taagattaac
     6781 acgaagtcat cgcaacagcg gacatgagtt acgaaagctt gcaattctat ctgtagccac
     6841 cgccgttgtc tttaagattc aggagcgtag tgcatggagt ttagtgtaaa aagcggtagc
     6901 ccggagaaac agcggagtgc ctgcatcgtc gtgggcgtct tcgaaccacg tcgcctttct
     6961 ccgattgcag aacagctcga taaaatcagc gatgggtaca tcagcgccct gctacgccgg
     7021 ggcgaactgg aaggaaaacc ggggcagaca ttgttgctgc accatgttcc gaatgtactt
     7081 tccgagcgaa ttctccttat tggttgcggc aaagaacgtg agctggatga gcgtcagtac
     7141 aagcaggtta ttcagaaaac cattaatacg ctgaatgata ctggctcgat ggaagcggtc
     7201 tgctttctga ctgaactgca cgttaaaggc cgtaacaact actggaaagt gcgtcaggct
     7261 gtcgagacag caaaagagac gctctacagt ttcgatcagt tgaaaacgaa taagagcgaa
     7321 ccgcgtcgtc cgctgcgtaa aatggtgttc aacgtgccga cccgccgtga actgaccagc
     7381 ggtgagcgcg cgatccagca cggtcaggcg attgccgccg ggataaaagc agcgaaagat
     7441 ctcggtaata tgccgccgaa tatctgtaac gccgcttatc tcgcctctca ggcgcgccag
     7501 ctggctgaca gctacagcaa gaatgtcatc acccgcgtta tcggcgaaca gcagatgaaa
     7561 gagctgggaa tgcactctta tctggcggtc ggtcagggtt cacaaaacga atcgctgatg
     7621 tcggtgattg agtacaaagg caacgcgtcg gaagatgcac gcccaatcgt gctggtgggt
     7681 aaaggtttaa ccttcgactc cggcggtatc tccatcaagc cttcagaagg catggatgag
     7741 atgaagtacg atatgtgcgg cgcggcggca gtttacggcg tgatgcgtat ggtcgcggag
     7801 ctgcaactgc cgattaacgt tattggcgtg ctggcaggct gtgaaaacat gcctggcggg
     7861 cgtgcctatc gtccgggcga tgtgttaacc accatgtccg gtcaaaccgt tgaagtgctg
     7921 aacaccgatg ccgaaggccg cctggtactg tgcgacgtgt taacttacgt tgaacgtttt
     7981 gagccggaag cagtgattga cgtggcgacg ctgaccggtg cctgcgtgat cgcgctgggt
     8041 catcatatta ctggtctgat gacgaaccat aatccgctgg cccatgaact gattgccgcg
     8101 tctgaacaat ccggtgaccg cgcatggcgc ttaccgctgg gtgacgagta tcaggaacag
     8161 ctggagtcca attttgccga tatggcgaac attggcggtc gtcctggtgg ggcgattacc
     8221 gcaggttgct tcctgtcacg ctttacccgt aagtataact gggcgcacct ggatattgca
     8281 ggaaccgcct ggcgttctgg taaagcaaaa ggcgcaaccg gtcgtccggt agcgttgctg
     8341 gcacagttcc tgttaaaccg cgctgggttt aacggcgaag agtaagtttg cgttttaccc
     8401 tcaccctaac cctctcccta agggagaggg aaccgatcga gcgcaataga aattcattgc
     8461 tcatctttca gggtgaggaa tgcagcaata tttaaatcca ccacaagaag ccccatatat
     8521 gaaaaacgca acgttctacc ttctggacaa tgacaccacc gtcgatggct taagcgccgt
     8581 tgagcaactg gtgtgtgaaa ttgccgcaga acgttggcgc agcggtaagc gcgtactcat
     8641 cgcctgtgaa gatgaaaagc aggcttaccg gctggatgaa gccctgtggg cgcgtccggc
     8701 agaaagcttt gttccgcata atttagcggg agaaggaccg cgcggcagtg cgccggtgga
     8761 gatcgcctgg ccgcaaaaac gcagcagcag cccgcgcgat atattgatta gtctgcgaac
     8821 aagctttgca gattttgcca ccgctttcac agaagtggta gacttcgttc cttatgaaga
     8881 ttctctgaaa caactggcgc gcgaacgcta taaagcctac cgcgtggctg gtttcaacct
     8941 gaatacggca acctggaaat aatggaaaag acatacaacc cacaagatat cgaacagccg
     9001 ctttacgagc actgggaaaa gcagggctac tttaagccta atggcgatga aagccaggaa
     9061 agtttctgca tcatgatccc gccgccgaac gtcaccggca gtttgcatat gggtcacgcc
     9121 ttccagcaaa ccatcatgga taccatgatc cgctatcagc gcatgcaggg caaaaacacc
     9181 ctgtggcagg tcggtactga ccacgccggg atcgctaccc agatggtcgt tgagcgcaag
     9241 attgccgcag aagaaggtaa aacccgtcac gactacggcc gcgaagcttt catcgacaaa
     9301 atctgggaat ggaaagcgga atctggcggc accattaccc gtcagatgcg ccgtctcggc
     9361 aactccgtcg actgggagcg tgaacgcttc accatggacg aaggcctgtc caatgcggtg
     9421 aaagaagttt tcgttcgtct gtataaagaa gacctgattt accgtggcaa gcgcctggta
     9481 aactgggacc cgaaactgcg taccgctatt tctgacctgg aagtggaaaa ccgcgaatcg
     9541 aaaggttcga tgtggcacat ccgctatccg ctggctgacg gcgcgaaaac cgcagacggt
     9601 aaagattatc tggtggttgc gaccactcgc ccggaaaccc tgctgggcga taccggtgta
     9661 gctgttaacc cggaagatcc gcgttacaaa gatctgattg gcaaatatgt cattctgccg
     9721 ctggttaacc gtcgtattcc gatcgttggc gacgaacacg ccgacatgga aaaaggcacc
     9781 ggctgcgtga agatcacacc ggcgcacgac tttaacgact atgaagtcgg taaacgtcac
     9841 gccctgccga tgatcaacat cctgaccttt gacggtgata tccgtgaaag cgcccaggtg
     9901 ttcgatacca aaggtaacga atctgacgtt tattccagcg agatcccggc agagttccag
     9961 aaactcgagc gttttgctgc acgtaaagca gtcgttgctg cggttgatgc actcggcctg
    10021 ctggaagaaa ttaaaccgca cgacctgacc gttccttacg gcgaccgtgg cggcgtggtt
    10081 atcgaaccga tgctgaccga ccagtggtac gtgcgtgccg atgtactggc gaaaccggca
    10141 gttgaagcgg ttgagaacgg cgacattcag ttcgtaccga agcagtacga aaacatgtac
    10201 ttctcctgga tgcgcgatat tcaggactgg tgtatctctc gtcagctgtg gtggggtcac
    10261 cgtatcccgg catggtatga cgaagcgggt aatgtttatg ttggccgcaa cgaagaagaa
    10321 gtgcgcaagg aaaataacct cggtgccaac gttgccctgc gtcaggacga agacgttctc
    10381 gacacctggt tctcttctgc actgtggacc ttctctaccc ttggctggcc ggaaaatacc
    10441 gacgccctgc gtcagttcca cccaaccagc gtgatggtat ctggtttcga catcatcttc
    10501 ttctggattg cccgcatgat catgatgacc atgcacttca tcaaagatga aaatggcaaa
    10561 ccgcaggtgc cgttccacac tgtttacatg accggtctga ttcgtgacga cgaaggtcag
    10621 aagatgtcca aatccaaggg taacgttatt gacccgctgg atatggttga cggtatttcg
    10681 ctgccagaac tgctggaaaa acgcaccggc aatatgatgc agccgcagct ggcggacaaa
    10741 atccgtaagc gcaccgagaa acagttcccg aacggcattg agccgcacgg caccgatgcc
    10801 ctgcgcttca ccctggcggc gctggcgtct accggtcgtg acatcaactg ggatatgaag
    10861 cgtctggaag gctaccgtaa cttctgtaac aagctgtgga acgccagccg ctttgtgctg
    10921 atgaacacag aaggtcagga ttgcggcttc aacggcggcg aaatgacgct atcgctggcg
    10981 gaccgctgga ttctggcgga gttcaaccag actatcaaag cgtaccgcga agcgctggac
    11041 agcttccgct tcgatatcgc cgcaggcatt ttgtatgagt tcacctggaa ccagttctgc
    11101 gactggtatc tggagctgac caagccggta atgaacggtg gaacagaagc cgaactgcgc
    11161 ggtactcgcc atacgctggt gactgtactg gaaggtctgc tgcgcctcgc gcatccgatc
    11221 attccgttca tcaccgaaac catctggcag cgtgtgaaag tactttgcgg tatcactgcc
    11281 gacaccatca tgctgcagcc gttcccgcag tatgatgcat ctcaggttga tgaagccgca
    11341 ctggccgaca ccgagtggct gaaacaggcg atcgttgctg tacgtaacat ccgtgcagaa
    11401 atgaacatcg cgccgggcaa accgctggag ctgctgctgc gtggttgcag cgcggatgca
    11461 gaacgtcgcg taaatgaaaa ccgtggcttc ctgcaaacgc tggcgcgtct ggaaagcatc
    11521 accgtgctgc ctgccgatga caaaggtccg gtttccgtta ccaagatcat cgacggcgca
    11581 gagctgctga tcccgatggc tggcctcatc aacaaagaag atgagctggc gcgcctggcg
    11641 aaagaagtgg cgaagatcga aggtgaaatc agccgcatcg agaacaagct ggcaaatgaa
    11701 ggctttgtcg cccgcgcacc ggaagcggtt atcgcgaaag agcgtgagaa gctggaaggt
    11761 tatgcagaag cgaaagccaa gctgattgaa cagcaggctg ttatcgccgc actgtaatca
    11821 acacagtgat gaaaacgaag gccggagcat gctccggcct tttttatctc ttacagaaat
    11881 ggtaaagaag gcataactcc ccgagaaata ctggctaaga agcctttatc tggttgttct
    11941 tctttattga ttaaaggaag gtcatccaaa tcgcccagca aatacgtatt ttttgcctgc
    12001 cagtcaatca tccatatttt cagcagtggg taagccagac cacccgtaat cccggatatc
    12061 accaccaacg cacacatgcg ataaagcata ccgtggtacg ttaaagttga gcgaaaacga
    12121 atacgcccat cattcagtga caggttgctc ataaaatgat ttcgcaacga caccgttaaa
    12181 taactggtgc taacagcaat cccaaaataa taaattaact gcgcgattat cattttacgt
    12241 tgcatttcca taaattgctg taaattctca atatcatcat ttgcataccc agaactatca
    12301 tactcattta atatttgatc gaagataata taaccagcaa cagcgagaaa aggtaataaa
    12361 gctaaaaatg cgaggatggc atattttata caatacgcag tatctaattt caccttgaaa
    12421 cgatgtatac cgaaactggt attgctccag agaaaactca ttaccaggct atataaagta
    12481 ccattaaaaa taccaatgga aacaatacca actattgcca tcagaaccac agatataata
    12541 acactacttg aactattggc atgtagcatc tttgtcgaga taaagaaaac agtccccatc
    12601 ccaatggcca ttaaaatcgg caagaaaaag gttacccacc agaacccttt caaagagcaa
    12661 ttaaaactaa atcttacgcc gttgagactg gtcattaagg cctgataacg cagtcctttt
    12721 gctgccataa atataagcaa aaccaacagt aacaaagtca aaacacagcc aacaagcggc
    12781 atatctgctg acactgtcat taagattgcg aaatagaaaa aaacaaaaac aagacaacta
    12841 acaaaaacat tcccaccggt aattccataa gaaaatcgtt gtccgttaac ttccatatta
    12901 gcataaagat aacgcttaca tttcattaat gcccatggta aatagatacc tagcgttata
    12961 atcgttaaca acacattcac cacacaaata agaaaatatc tctcacctgt accatgaaaa
    13021 acaaaagaat gggacggaac atccatttca ttaataactt gagccataaa aatcccctga
    13081 attaaaaaat ccattgatat aatttataga tacagacagt aaaatgttct gccacgttac
    13141 aatactctta tcagtaatag gctggcaaaa actttattgc tttataagac gcttgcgcac
    13201 gtaagaagag agtggtatta aacaaatgta taattacttt atttttattg ggttaaacta
    13261 tgaataacat tgcgccgcaa tcacctgtaa tgcgtcgcct gacgctgcag gataatcctg
    13321 ctatcgccag cgtcatccgt caggtttacg cagagtatgg tcttaccgcc gataaaggct
    13381 acaccgttgc cgatccgaat ctcgacgaac tgtatcaagt gtacagccag cctggccatg
    13441 cttattgggt ggttgaatac aatggcgaag tggtcggcgg cggcgggatt gcgccattaa
    13501 caggtagtga agcggacatt tgcgaactgc agaaaatgta ctttcttccc gctattcgcg
    13561 gtaaagggct agcgaaaaaa ctggccttaa tggcgatgga gcaggcacga gagatgggct
    13621 tcaaacgctg ctatctggaa acgaccgctt ttttaaagga agccattgcg ctttatgagc
    13681 atttgggctt tgagcatatc gactatgcgc ttggctgcac gggccatgtc gattgtgaag
    13741 tgcggatgct acgcgaactc taaaaaaatg cccgctagta tgtgctatcg ggcattttaa
    13801 tcaattaatt agtggcgaac cccgtcatcg tcttcatcga caaaatcttc atcgtcgcca
    13861 tcttcgccgt taggatcttc aaagtaagtg ccccaaccgt cgtactcaac gtcaaatttc
    13921 tccgccagcg tcatcagttg ttcaacctgg gcatcgatca gatcggcatt caacgcgcac
    13981 tcgctgagga tgtcgcagca aatcacgata tcaccatctt caacttccag ctcttctgga
    14041 tcggtcactt cgtaaccgag tttaaacgct tcaactgccg ctttttccag agtttccaga
    14101 tcgtctgcgg aaagatggtg ttcgatggtg tacagcgcgt ccggatcgct gccatcttcc
    14161 agtaattctt caataatcaa acgtgtttct tcacgctgtt cttccagttg ttccgggttt
    14221 gccatggctc attcctctta atgtcctgcc gatactctta ttgtcacaca ccactgacat
    14281 tgcctccacc tttgtgacaa agatttatgc tttagacttg caaatgaata ataatacaca
    14341 taaattgagt tttaattcat tgaggcgtta gccacaggag ggatctatgt ccgggtttta
    14401 tcataagcat ttcctgaaat tactcgattt cacgccagct gaactcaaca gcctgctgca
    14461 gttagccgcg aagctgaaag ctgataagaa aagcggtaaa gaagaagcca aactcactgg
    14521 taaaaacatc gcgctcatct tcgaaaaaga ctcgactcgt acccgatgct ctttcgaagt
    14581 tgccgcatat gaccaggggg ctcgcgttac ttatcttggc ccaagcggta gtcagattgg
    14641 tcataaagag tcgattaaag acactgcccg cgtgcttggt cgcatgtatg acggtattca
    14701 gtatcgcggc tatggtcagg agattgtcga aacactggcg gaatacgctg gcgtgccggt
    14761 atggaacggc ctgaccaatg agtttcaccc cacgcagttg ctggcggatc tcctcaccat
    14821 gcaggagcat ttacccggta aagcattcaa cgaaatgacg ctggtctatg caggtgacgc
    14881 acgtaacaac atgggcaatt cgatgctcga agctgcggcg cttaccggtc tggatttgcg
    14941 tctggtcgcg ccacaggcat gctggccaga agctgcgctg gttacggaat gccgcgccct
    15001 ggcacaacaa aatggcggga atattacgct gacggaagat gtagctaaag gagttgaagg
    15061 tgctgacttt atctataccg atgtatgggt gtcgatgggt gaagcaaaag aaaaatgggc
    15121 tgaacgcatt gcattactgc gtgattatca ggtgaacagc aagatgatgc agttgaccgg
    15181 taacccggag gtcaaattcc tccattgcct gcccgcgttt catgacgacc aaacgacgct
    15241 tggcaaaaaa atggcggaag agtttggcct acatggcgga atggaagtga ctgatgaggt
    15301 cttcgaatct gccgccagca ttgtatttga tcaggcggaa aaccgcatgc ataccatcaa
    15361 agcggtgatg gtcgcgacgc tcagtaaatt aaataattaa tatctctata aa
//
LOCUS       DAZPCS010000087        15035 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZPCS010000087 DAZPCS010000000
VERSION     DAZPCS010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15035
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.087"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..178
                     /gene="yjbD"
                     /locus_tag="ACHUQ7_002596"
     CDS             <1..178
                     /gene="yjbD"
                     /locus_tag="ACHUQ7_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFR1135951.1"
                     /translation="VLTNSETNSIKKEYIDKLMVEREAEAKKARQLKKKQAYKPDPEA
                     SFSWSANTSTRGRR"
     gene            complement(311..1183)
                     /gene="rluF"
                     /locus_tag="ACHUQ7_002597"
     CDS             complement(311..1183)
                     /gene="rluF"
                     /locus_tag="ACHUQ7_002597"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFR1135952.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            1531..2478
                     /locus_tag="ACHUQ7_002598"
     CDS             1531..2478
                     /locus_tag="ACHUQ7_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFR1135953.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDIALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            2548..3354
                     /locus_tag="ACHUQ7_002599"
     CDS             2548..3354
                     /locus_tag="ACHUQ7_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR1135954.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            3364..3771
                     /locus_tag="ACHUQ7_002600"
     CDS             3364..3771
                     /locus_tag="ACHUQ7_002600"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HFR1135955.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVAFVPGENAGDIA
                     VKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            3771..4265
                     /locus_tag="ACHUQ7_002601"
     CDS             3771..4265
                     /locus_tag="ACHUQ7_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFR1135956.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            4331..5128
                     /locus_tag="ACHUQ7_002602"
     CDS             4331..5128
                     /locus_tag="ACHUQ7_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFR1135957.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALCWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWCKAEPQLQATASTALDQLDD"
     gene            5139..5927
                     /locus_tag="ACHUQ7_002603"
     CDS             5139..5927
                     /locus_tag="ACHUQ7_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IID"
                     /protein_id="HFR1135958.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRLHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            5979..7208
                     /gene="sorE"
                     /locus_tag="ACHUQ7_002604"
     CDS             5979..7208
                     /gene="sorE"
                     /locus_tag="ACHUQ7_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HFR1135959.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVTTNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVDGEATHVVIPNEVMEQYCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGHTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEVEQYLLAHAEAISHD"
     gene            7590..8279
                     /gene="pepE"
                     /locus_tag="ACHUQ7_002605"
     CDS             7590..8279
                     /gene="pepE"
                     /locus_tag="ACHUQ7_002605"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFR1135960.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(8370..10001)
                     /locus_tag="ACHUQ7_002606"
     CDS             complement(8370..10001)
                     /locus_tag="ACHUQ7_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFR1135961.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(10221..13904)
                     /gene="metH"
                     /locus_tag="ACHUQ7_002607"
     CDS             complement(10221..13904)
                     /gene="metH"
                     /locus_tag="ACHUQ7_002607"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFR1135962.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLA
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFAIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTCKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDHA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            14104..14928
                     /gene="iclR"
                     /locus_tag="ACHUQ7_002608"
     CDS             14104..14928
                     /gene="iclR"
                     /locus_tag="ACHUQ7_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFR1135963.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         3563 a         3968 c         3938 g         3566 t
ORIGIN      
        1 cgtactgacc aactccgaaa ccaacagcat taagaaagag tacatcgata aattaatggt
       61 cgagcgtgag gctgaagcga aaaaagcccg ccaattgaag aaaaagcagg cttataaacc
      121 ggatcctgaa gcgtcattta gctggtcagc aaatacgtcg acgcgcggca ggcgctaatg
      181 aacaatcaac gcctgagatt ttcactcgtc tgtgtggaca caatcttccc ctcaccctaa
      241 ccctctcccc gaaggggcga ggggactgtc cgagctcttt ttggacgttt tcatcagtct
      301 gaggcgttaa tcagcgtccc ttctttttac gccccggcga ggtaaaacgc ttaccgttgg
      361 acgccgggcg accgcctttt tccgccgttt tttccatctt aacgactgga cgtttgatgc
      421 ccgctgtttt cggtttggcc ttcgctttag gttttgcctc ggaagaggaa ttttcgatga
      481 gcttaaagag gtcgattaac tcatcgtcgg ttaaatcgcg ccactccccc agcggaatgc
      541 cgcttaagct gacgttcata atgcgcgtac gttccagctt tttcacttca tagccgaaat
      601 gctcgcacat gcgccggatc tgacggttca gcccctgcac tagggtaatg cggaagacaa
      661 acggcgcttc ttttttgact ttgcactttt tcgtcaccgt cccgaggatc ggcacccccg
      721 cccccatacc acgaataaat tcatcggtaa tcggtttatc gaccgtcacc agatactctt
      781 tctcatgatc gttacccgca cggaggatct tattcaccag atcgccgtga ttggtgagga
      841 aaatcagccc ctgggagtct ttatccaggc ggccaatcgg gaacacgcgt ttgctgtggt
      901 taacgaaatc gacaatgtta tcgcgctcgc catcttcggt ggtgctgaca ataccgacgg
      961 gcttgttcag ggcgataagt accaaatctt cggcttcccg aggctcaatc aactgaccgt
     1021 ttactttcac aatgtcgccg ggtttcacct gatcgccaat ggtggctcgc ttgccattaa
     1081 ggaacacatt gccttgctcg atatagcgat ccgcttcgcg gcgtgagcaa attccgcttt
     1141 cgctgatgta tttatttaaa cggactgatg agtcgggcag cataaattct cctgtaaaac
     1201 gcggaatata ccgtacctct tagcggggaa aaacagggtt atgcggcaac gtcgaaatta
     1261 aatgtgatct gccacgcctt ttatcacaaa agtgtctccg gaagtacgat gcaaatttgc
     1321 ctggcaaaat aaggataatc aacatattac gaatcgctgc acacctgtgc tgatgactac
     1381 tacgcagaga aacaaatgtg caagagaaag cgctatttct ccccatgcaa agagtgaaaa
     1441 atcgctttga aaggccctca tgatggggtt atatcagatc aagcggtgag aaagcgtatt
     1501 tgcacaaatg tgcagaacca ggaggcggca atggaaaaca gtgacgatat ccgtttgatt
     1561 gtgaagattg cccaactcta ttacgaacag gatatgacgc aggcgcaaat cgcgcgcgaa
     1621 ctgggtattt accgcaccac catcagccgc ttgcttaaac gaggccgcga tcagggaatg
     1681 gtcaccatcg ccatcaacta tgactacaac gaaaatctct ggctggagca gcaactgaag
     1741 caaaagtttg gcctgaaaga cgttgtggtg gtgtcgggaa atgatgagga tgaagagact
     1801 caactggcga tgatggggtt acacggcgcg caactgctgg atcgcttgct ggagcctggc
     1861 gatattgtcg gtttttcctg gggtcgcgcg gtgagcgcac tggttgaaaa cttgccgcag
     1921 gcggggcaat cgcggcagtt aatctgcgta ccaattattg gcggaccgtc cggtaaactc
     1981 gaaagccgct atcatgtaaa cacattaacc tacagcgcgg cagcgaagct gaaaggggaa
     2041 tcgcatctcg cggattttcc ggctctgctg gataacccat taattcgtaa tgggatcatg
     2101 cagtctcagc actttaaaac catctctgcc tactgggata atctggatat cgccctggtg
     2161 ggaattggct caccggccat tcgcgacggc gctaactggc atgcgtttta tggtggtgaa
     2221 gagagtgacg acctgaatgc ccgccaggtt gctggcgata tttgctcgcg cttttttgat
     2281 attcacggcg caatggttga aacgaatatg agcgaaaaaa cgctctctat cgaaatgaat
     2341 aaattaaagc aggcgcgata ttccattggc attgccatga gtgaagaaaa atacagcgga
     2401 attattggtg cactgcgtgg aaaatatatt aattgtctgg taacgaatag cagcacagct
     2461 gaactattac tgaaataaaa atcttcccga tgtttaccgt aataaaaatt acggcgggtc
     2521 cctattatct aaaatcagga gtctgttatg caaacgtggt taaatttgca ggataaaatc
     2581 attattgtca ccggcggcgc atccggtatt ggtctggcga ttgtagagga attattagca
     2641 caaggcgcga atgtacagat ggtcgatatt cacggtggcg atggtcaata tgaaggtcat
     2701 aaaggctatc agttttggcc gaccgatatt tctagcacaa aagaggtaaa tcatacggta
     2761 gcggaaatta tccagcgttt tggtcgcatc gacggtctgg tcaataacgc cggggtcaat
     2821 ttcccgcgtc tgctggtcga tgagaaagcg cctgccgggc agtatgaact caacgaagct
     2881 gcattcgaaa aaatggtcaa tatcaaccag aaaggtgttt ttctgatgtc gcaggcggtg
     2941 gcgcgacaga tggtcaaaca acatgatggc gtgattgtga atgtttcctc agaaagtggg
     3001 ctggaaggct cagaaggaca aagctgttac gccgctacca aagccgcgct caatagcttc
     3061 acgcgctcct ggtcgaaaga gctgggtaaa cacggtatcc gtgtggtcgg tatcgcgccc
     3121 gggattctgg aaaaaaccgg gctacgcacg ccggaatatg aagaggcgct ggcgtggacg
     3181 cgcaatatca ccgtcgagca gctgcgtgaa ggctatacca aaaacgccat tcctattggg
     3241 cgcgccggaa gattagcaga agtagctgat tttgtttgtt atctgttgtc tgaacgcgcc
     3301 agctacatca ccggagtaac cactaacatt gcgggcggca aaacgcgcgg ctaaggaggc
     3361 tttatggtca acgccatttt ttgtgcccac ggcaaattag cttgcgccat gctggaatcg
     3421 gttcagatgg tttacggcaa cgccaatgtt gaagccgttg catttgtgcc cggtgaaaac
     3481 gccggagaca ttgcggtaaa gctggaaaag ttagtaagca ttcacaatca agatgagtgg
     3541 ctgattgccg tggatttaca gtgcggcagc ccgtggaatg cggcagcaat gcttgcgatg
     3601 cgcaatcctc gtttgcgggt aatcagcggc ctttctctgc cgctggcgct cgaactggtt
     3661 gataaccagg acagcatgaa tgttgatgaa ctgtgtgaac acctgacgca aatcgcaaag
     3721 caaacctgcg ttgtctggaa acaactggca acgacagagg aggatttctg atgaacatta
     3781 cgctcgcccg cattgatgac cgtctgatcc acggccaggt cactaccgtc tggtcgaaag
     3841 tggctaacgc ccagcgcatt attatctgca atgacgaagt ttataacgat gaagtgcgcc
     3901 ggacattatt gcgtcaggct gcgccaccgg gaatgaaggt taacgtggtc aatattgaaa
     3961 aagccgtcgc ggtttatcac aacccgcaat atcaggacga aaccgttttt tatcttttca
     4021 ctcgcccaca ggatgcctta gcgatggttc gccagggggt aaaaataggc accctgaata
     4081 ttggcggcat ggcctggcgg cccggtaaaa aacagttaac caaagcggtt tcattagatg
     4141 atgatgatat taacgcattt catgagttaa ataatcttgg tgtcatactg gatttacgcg
     4201 tcgtagcgtc agatccctca atcaatatta tcgacaaaat aaacgaacag ttaatcgcaa
     4261 attaaataat agcgcctgtg tacatatgct ttaacaggca gggatgacga cacttaaaag
     4321 gtgacaaatc atggaaataa gtaccctaca aatcattgct atatttcttt tttcctgtat
     4381 tgccggaatg ggcagcgtgc tggatgaatt tcagactcat cgcccgttaa ttgcctgtac
     4441 ggtgattggt ttaattctcg gtgatttaaa aaccggaata atactcggcg gtacgctgga
     4501 attgatagct ctctgctgga tgaacgtcgg cgcggcgcaa tctccggatt ctgcactcgc
     4561 cagcataatc tccgccattc tggttatcgt tggtcagcaa agcatcgcca ccggaatcgc
     4621 catcgcgttg cctgtggctg cggcaggcca ggtgctgacg gtgtttgccc gtaccatcac
     4681 cgtagtgttc cagcacgcgg cggataaagc agcagaagag gcgcggtttc gcactctcga
     4741 tattctgcat gtctccgcgc ttggcgtgca ggcgctgcgc gtcgccattc cggcactgat
     4801 tgtctcactg ttcgtcagcg ccgatatggt cagcaatatg ttaaacgcca ttcccgaatt
     4861 tgtgacccga ggactacaga ttgctggcgg ttttatcgtg gtggtcggtt acgccatggt
     4921 gctacgtatg atgggcgtga aatatttgat gcctttcttt ttcctcggtt tcctcgcagg
     4981 tggctacctc gatctcagcc tgctggcgtt cggtggcgtc ggcgtgatca tggccctggt
     5041 ctacatccag ctaaatccac agtggtgtaa agctgaacct caactccagg ccaccgcctc
     5101 caccgccctt gaccaacttg acgattaacg gagccatcat ggaacagaga aaaattacac
     5161 gcagcgatct ggtgagcatg tttctgcgct ccaacctgca acaggcgtcc tttaactttg
     5221 aacgtattca cgggctgggc ttttgctacg acatgatccc cgccatcaag cgactttacc
     5281 cattaaaaga ggatcaggtt gcggcgctca ggctacacct ggtgttcttc aataccacgc
     5341 cagccgtatg tggcccggtc atcggcgcgg aaattgatga cggtaccatc aacggcatca
     5401 aagtcggtct gatgggacca ttggcaggag ttggcgatcc actggtctgg ggaacgctgc
     5461 gcccgattac cgccgcgctc ggcgcatctc tggcactttc gggcaacatt ctcggcccgc
     5521 tgctgttctt ctttattttc aacgcggtgc gtctggcgat gaagtggtat ggcctacagc
     5581 tcggctttcg caaaggggtg aatatcgtca gcgatatggg cgggaatgtg ctgcaaaaac
     5641 tcaccgaagg cgcgtcgatt ctcggactgt ttgtgatggg cgtgctggtg accaaatgga
     5701 cgtcaatcaa cgtaccgttg gtggtttcac aaacgcatgc cgccgatggc tccaccgtca
     5761 ccatgaccgt gcagaacatt ctcgaccaac tttgccctgg tttgctggcg ctcggtctga
     5821 cgctactaat ggttcgtctg ctcaacaaaa aaattaaccc ggtatggctg attttcgccc
     5881 tgtttggctt agggattatc ggcaatgctc tgggcttcct gtcctgattc ttcgccccgg
     5941 cacgactgcc gaggccatcg ctcaacatga ggtggtttat gaaaacaaca gctctgcgtc
     6001 tttatggtaa acgtgattta cgcctggaaa cctttgacct tcctgaaatg caggaggatg
     6061 aaatcctcgc aacggtggtc actgacagcc tgtgcctctc ttcctggaaa gaggccaatc
     6121 tgggtgaaaa ccataaaaaa gtacccgacg atgtgacgac caacccaatc atcatcggcc
     6181 acgaattttg cggcgatatt ctggccgtgg gtaaaaagtg gcagcacaaa ttccagccgg
     6241 gtcagcgtta tgtgattcag gccaacctgc aactccccga ccgcccggat tgccccggct
     6301 actccttccc gtgggtagac ggcgaagcga cgcatgtggt tattcccaac gaggtcatgg
     6361 aacaatattg cctgctggca tacgacggcg aaacctattt tgaaggctca ctggttgaac
     6421 cgctttcctg cgtgattggc gcgttcaacg ccaactatca tcttcaggaa ggtagttata
     6481 accacacgat ggggattcgc ccgcaagggc acacgctgat cctcggcggc accggaccaa
     6541 tgggactgtt ggcgattgat tatgcgctgc atggaccagt taacccgtcg ctgctcgtca
     6601 ttaccgatac cgacaacgac aaattgagtt atgcgcgcaa gcactatcca tcagaaccgc
     6661 aaacactgat tcattatctc aatgccaccg atgcagcatt tgatacgtta atggcgctga
     6721 gtggcggtca cggcttcgat gatattttcg tctttgtgcc taatgaagga ctggtgactc
     6781 tcgcctcttc cttgctggcg acagatggtt gcctgaattt cttcgccgga ccgcaggata
     6841 aacatttcag cgcgccaatt aatttctacg atgtgcatta tgcatttacc cactacgtgg
     6901 gcacgtcagg cggcaatacc gacgacatgc gcgcggcggt caaactgatc gaagagaaaa
     6961 aagtgcaggc cgcaaaagtg gtaacacata ttcttgggct gaatgccgcg ggcgaaacca
     7021 cgcttgaatt gcctgccgtc ggcggcggca aaaagctggt gtataccggg aaatacctgc
     7081 cgctgacgtc actcacgcag attcaggatc aagcactggc ggcgattctg gcgcgtcatc
     7141 aggggatctg gtccggtgag gtggagcaat acctgctcgc ccatgcagag gcaatttccc
     7201 atgattaatc gcgctactta actctgcatg tcgctggctg gtcgccccag caacttcgcg
     7261 accggatgtg cggctgattg ctgaagcagc acaatttgca cgagaaggat aaaaaatatc
     7321 agcgccttaa ggggcgctca aattggtggc aaagtacaca ttgtttatgc cggatgcgga
     7381 atgaagcctt attcggtcta cataattttg aaaatccaat atattgcagt tatctggtag
     7441 gccaggtaag cgtagcgcat caggcaatgc tgtgtttgtc atcagtctca gcgcccggtg
     7501 caatacgtga catcgtgagc catgtcagta agtggttaaa ttcgcagtgg tagcatgcct
     7561 tccgtgattt cttactaacc ggagaagtaa tggaactgct tttattgagt aactcgacgc
     7621 tgccgggtaa agcctggctg gaacatgcac tgccgctaat tgctgaacag ttgcagggtc
     7681 gccgctcagc ggtgtttatc cctttcgctg gcgtaacgca gacctgggat gattacacag
     7741 agaaaacggc tgcggttctc gctccgctgg gtgtttctgt caccggtatt catagcgttg
     7801 tcgatcccgt tgccgcgatt gaaaatgcgg aaattgtgat tgtcggcggc ggtaatactt
     7861 tccagttgct gaaacagtgc cgcgagcgcg ggctgctggc tccaattact gacgtggtta
     7921 aacgtggcgc gctgtatatt ggctggagcg caggcgctaa ccttgcttgc ccaaccattc
     7981 gtaccaccaa cgatatgccg attgtcgatc cgcaaggttt cgatgcgcta aatctgttcc
     8041 cgttgcaaat caacccgcac ttcaccaacg cgctgccgga aggccataaa ggtgaaaccc
     8101 gtgagcagcg tattcgcgaa ctgctggtcg tcgcgccaga actgacgatt attggtctgc
     8161 cggaaggtaa ctggatcaca gtgagtaaag gtcacgctac gctgggtggc ccgaacacca
     8221 cttatgtgtt taaggctggt gaagaagcgg ttccgctgga agctggtcac cgtttttaag
     8281 tgaattgccg gatgcatgat ccggcatctt gttcatagtg cctgatgcga cgcttgacgc
     8341 gtcttatcag gcctacagga ttcacaatat taatactcgt cccgtccttc atcttcatcc
     8401 ggctgttcca gcacactgta agccaccgaa caaaacagcg agttcaggcg ctgcatatct
     8461 cccagtaagc ctaaatgtag cgaactggtt tcaatgctct gcacgttttg ctgatgcagg
     8521 cgatcgacgt gggcgtggga atagcggcga ttaagaatgc gaaaacgatg tttgctgcga
     8581 cgcaaacgac gagcgctggt gacatcgcca gagaagaaca ccgacattgc cagctttaaa
     8641 ttactgagca attgctcata gagcgcatcc agttccttca acccatcaag cgaaaatgcc
     8701 cgccgtgctg ccagcgattt atcggcaatt tcgctgccca tgcgctcgac gatatcggag
     8761 gcctgttcaa ggttgagcga catttcgatg atctccgccc agcggcgcga ctcttcctcc
     8821 gccaactctt cttttggcat ccgcgccaga tacagcttaa tggcggtgta gagaacgttg
     8881 atatcatctg ccagcttacg cagctctttc tcctgccgtg gctcgccgtg catcacttta
     8941 ttcagccctt ccatcatctg ttccatggcg tcgccaatgc gcagggtttc gcgcgcggcg
     9001 ttcgccagag caagcgtggg cgtgtccagc gcgctgacat ccagatgttt aggccgtagc
     9061 tgggtatcca gttccggttc atcgcgaata atcgttttgc aaaaccgtgc catcgggtca
     9121 acaaatggca gcatgaccag gcaacgtaca aggttgtaga agacgtggaa atagatcacc
     9181 agttccgctt ttggcaatga cagcttcccc attgtctctg ccagcaaatg gacaaacggc
     9241 aggataatca ggctacccac cagcttaaac agcagactac ccagcgcgac acggcgggct
     9301 gcggcattgg cggcactgtt gttgagcatc gccagcaggc cggaaccgag gttagctcca
     9361 atcaccagac agagcgccac ggggaaggag ataatgcctg cagcggtcag agtcgcggtc
     9421 agcagtacag cagcaaggct ggagtagctg ataatggcga acatcgcgcc aatcagcgca
     9481 tccagcagaa tatcgccggt cagcgaggca aagatcacct gaacgccgtt tgcctgggtg
     9541 atcggcgtta cggcctgcac aatcaactcc agcgccagca aaatcagccc aagaccaata
     9601 ccgacgcggc ccagttgccc ggcgcgtgac tgtttgcgtc cgaggaaaaa aatcacgccg
     9661 ataaaaataa gtaacggtga cagccaggat aagtcgaagg tgagaatacg cgccattagc
     9721 gccgtcccga catctgcccc cagcacaatg accagagccg gtgcgagggc taccagatcc
     9781 tgagcgacga acgaggtcac cagcatggtg gtggcattac tgctctgtac cagtgcggta
     9841 acgccgatcc ccgcgcaaaa ggcgagcggc tttttttcga cgctccggct aaggacagta
     9901 cgcaaacgcg cgccgaagac gcgcattacg ccggttcgaa caatatgagt cccccagacc
     9961 agcagggcga cggcagaaag caggtgaagc agcgttaaca cgagttcaga ttctccttat
    10021 cgttatatgt tcctgaagca attgaatgac caaaggaccg gaaacacgct gactggcatc
    10081 tttactgtgc cttttgacat tcgtttttcc ggctaagatt attccagcct gtttccagta
    10141 taagtgtttc atattaataa agagacaggg cactcattga gcgccctgtt tgttgtaagg
    10201 aaaagtgaca gatttgtgaa tcagtccgcg tcatacccca gattcggtgc cagccagcgc
    10261 tcaacatcgg agacgctcat acctttacgg cgggcatggt cttcaacctg atcgcgctga
    10321 atttgcgcca ccgcgtagta cttgctgtcc ggatgactga aataccaacc cgaaaccgat
    10381 gcaccaggcc acatcgcgaa agattctgtg agtttcatgc cagtatgttt ttccacttcc
    10441 agcagctccc agatggtggc tttttccgta tgttccgggc aggccggata gcccggtgcc
    10501 ggacggatgc cctggtagtt ttcgcggatc agttcttcgt tgctgaggtt ctcgttcggc
    10561 gcatagcccc agtagacttt acgcacacgc tcatggagat actccgcaaa ggcttcggct
    10621 aaacggtcgg caagcgcttt caccatgatt ttgttgtaat catcgtgctg cgcttcaaag
    10681 gcatcagcca gtgcgtcctc ttccagcccg ccagtcacgg caaatgcgcc gatgtaatct
    10741 gctttaccag aaagcttcgg cgcaacgaag tcagcgagac agtagttagc gaatcccgtt
    10801 ttttcggtct gttgacgcag atggtggctg acgttgatca catgggtgcg cgtttcgtca
    10861 cggtagattt caatgtcatc gcccacacgg tttgccggga acaggcccac cacgccacgc
    10921 ggattcagcg ttttctcggc gcttaattta tccagcatgt cgttggcgtc tttaaacagc
    10981 cgctgcgcct caacgcccac cacttcatct tccagaatgc gaggatactt cccggccagc
    11041 gaccaggtca taaagaacgg tgtccagtcg atgtaattac gcagcgtttc gatgctggct
    11101 tccacttcct gcacgccgag acggtgcgcc accggcggcg tgtaagcctg ccagtcaaat
    11161 gcgaaatcgt tatcgcgcgc cgcttccagc gtgaccggtg gtgtgcgtgg tttcttgcgc
    11221 ccgtgctgaa tacgcacggt ttcgtactcc ttgcaggtac gagcgacaaa atcatcacgc
    11281 tgagtatcgg aaagcagcgc cgctaccaca ccaacggtgc gcgaggcatt ctgcacatac
    11341 accgtagggc cgctgtagtt ctgctcgatt ttcaccgccg tgtgcgcttt tgaggtggtc
    11401 gcgccgccaa tcagtaacgg aatggcgaag ccctgacgct ccatctcttt cgccacgtta
    11461 accatctcgt ccagcgacgg cgtgataagc cccgaaaggc caatcaaatc agcattcact
    11521 tctttggcga tacggagaat tttttccgca ggcaccataa cgccgagatc gacaatttcg
    11581 tagttgttac attgcagcac cacaccaacg atatttttac cgatgtcgtg gacatcgcct
    11641 ttcacggtgg cgatcaccat cttgccgttg gttttaccct gctctttgct ggcttcaata
    11701 aacggttcga gatacgccac tgcctgtttc atgacgcgcg ccgatttgac cacctgtggc
    11761 aggaacattt tgccttcgcc aaacagatcg ccgaccacgt tcatgccgtc catcaacggg
    11821 ccttcaatca cttcaatcgg gcgcgtagcc tgctggcggg cttcttcagt atcctgctcg
    11881 ataaactcgg ttatgccttt gaccagcgag tattccagac gtttattcac gtcccacgag
    11941 cgccactccg cttgctgggc gttagcagtg tcgtccgctt tactgccgcg atatttctcg
    12001 gcaagctcca gtaaacgctc agtgccatcg tcacgacgat taagaatcac atcttccacc
    12061 gcgtcgcgca gttcagcggg taggtcgtcg taaatcgcca gttgcccggc gttgacgatc
    12121 cccatatcca tgccattgcg aatagcgtag tagaggaaca ctgcgtgaat ggcttcgcgc
    12181 accggatcgt tgccacggaa tgagaaagaa acgttagata cgccgccgga aatcagcgcg
    12241 tgcggcagtt cgcgtttgat gtcttcacac gcgccgataa agtcctgcgc gtagttgttg
    12301 tgctcttcaa taccggtggc gaccgcgaag atgttcgggt cgaagatgat atcttctggc
    12361 gggaagccaa cctcttcggt gaggattttg tacgcccgac ggcaaatctc gattttccgt
    12421 gcgcgagtat cggcctgtcc ctgttcgtca aaggccatca ccaccactgc cgcaccgtag
    12481 cgacgcaaca atttcgcgtg atggataaag gcgtcgacgc cctctttcat cgagatagag
    12541 ttaacaatgc ctttgccctg gatacacttc agaccttttt caatgacatc ccattttgag
    12601 gagtcgatca taatcggcac gcgagcgata tccggttcac cggcaatcag attgagaaaa
    12661 cgcaccatcg ccgcttcggc atcgagcatc ccttcatcca tgttgatatc gataatctgc
    12721 gcgccgtttt ccacctgctg acgcgccaca tccagcgcct cgctgtattt ctcttctttg
    12781 atcaggcgct tgaacttagc agagccggtg acgttggtgc gttcacccac gttcacaaac
    12841 aggctatcgt cgccaatgtt cagcggctcc aggccagaca aacggcaggc tacgggaatt
    12901 tccggcagtt tgcgcggcgc taatccttct actgcacgac tcatcgctgc aatatgttgc
    12961 ggcgtggtcc cgcagcaacc accgacgata ttgagaaagc ccgcttgcgc ccattcacgt
    13021 atctgttttg ccatcgtgtc ggcgtcgaga tcgtattcac caaaggcgtt aggcagcccg
    13081 gcgttcgggt gggcggtgac gtagcattcc gcaatccgtg atagttcctg cacgtactgg
    13141 cgcagttcat cgggccccag cgcacagttc aggccaaagg tcagcgcttc ggcgtggcgc
    13201 aatgagttgt aaaatgcttc ggtggtctgc ccggagagcg tgcgcccgga ggcgtcggtg
    13261 atggtgccgg agatcataat cggcagctca acgcccagcg cttcaaactc cgttttcacc
    13321 gcaaagaccg ccgctttggc gttaagggtg tcgaaaacgg tttcaatcag gatcagatcc
    13381 gcaccacctt ccaccagcgc tttggtggat tcgcgatagg ccgccgccag ctggtcaaaa
    13441 gtgatgttgc ggaatgccgg atcgttgacg tccggagaaa tagacgccgt gcggttggtt
    13501 gggccaagaa caccggcaac gtagcgcggt ttctccggcg tgcgcgcggt ccactcgtca
    13561 gcacaggcgc gcgccagttt cgccgccgca aagttgattt ccgccgacag ggattccatc
    13621 tggtaatccg ccatcgcaat ggtcgtggag ttgaaggtgt tggtttcgat gatatccgcg
    13681 cccgcttcaa agtaggcgtt gtggatagcg gcgatcactt ccggtttact gagtaccagc
    13741 aggtcgttgt tgcctttgag gtcgcatggc cagtcggcaa agcgttcacc acgaaaatcg
    13801 gcttcgttca gtcgatagct ctggatcatg gtgcccatac cgccgtccaa caccagaata
    13861 cgttcattta actgcgcacg cagttgttcc actttgctgc tcacacttgc tcccgacacg
    13921 cttaacccag atttaataaa aattcaacaa accatactgg aataaacgca tctgtggtaa
    13981 aagcgaccac cacgcaacat gagatttgtt caacattaac tcatcggatc agttcagcaa
    14041 ctattgcatt agctaacaat aaaaatgaaa ataatttcca cgatacagaa aaaggaggct
    14101 gtcatggtcg cacccattcc cgcgaaacgc ggcagaaaac ccgccgttgc caccgcacca
    14161 gcgactggac aggttcagtc tttaacgcgt ggcctgaaat tactggagtg gattgccgaa
    14221 tccaatggca gcgtggcact cacagagctg gcgcaacaag ccgggttacc caattccacg
    14281 acccaccgcc tgctaaccac gatgcaacag cagggtttcg tgcgtcaggt cggcgaactg
    14341 ggacactggg caatcggcgc acatgccttt atggtcggca gcagctttct ccagagccgt
    14401 aatttgttag caattgttca ccctatcctg cgcaatctaa tggaagagtc tggcgaaacg
    14461 gtcaatatgg cggtgcttga tcaaagcgat cacgaagcga ttattatcga ccaggtacag
    14521 tgtacccacc tgatgcgaat gtccgcgcct atcggcggta aattgccgat gcacgcttcc
    14581 ggtgcgggta aagccttttt agcccaactg agcgaagaac aggtgacgaa gctgctgcac
    14641 cgcaaagggt tacatgccta tacccacgca actctggtgt ctcctgtgca tttaaaagaa
    14701 gatctcgccc aaacgcgcaa acggggttat tcatttgacg atgaggaaca tgcgctgggg
    14761 ctacgttgcc ttgcagcgtg tattttcgat gagcaccgcg aaccgtttgc cgcaatttct
    14821 atttccggac cgatttcacg tattaccgat gatcgcgtga ccgagtttgg cgcgatggtg
    14881 attaaagcgg cgaaggaagt gacgctggcg tacggtggaa tgcgctgact ttttctggcg
    14941 ggcagaggca atattctgcc catcatacct gagtggcaat agaataaggg tgtctgttaa
    15001 tcgcattgac gccaaaataa cttaatgtca tacac
//
LOCUS       DAZPCS010000088        14906 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZPCS010000088 DAZPCS010000000
VERSION     DAZPCS010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14906
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.088"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            100..342
                     /gene="yjbE"
                     /locus_tag="ACHUQ7_002609"
     CDS             100..342
                     /gene="yjbE"
                     /locus_tag="ACHUQ7_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFR1135964.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            456..1094
                     /locus_tag="ACHUQ7_002610"
     CDS             456..1094
                     /locus_tag="ACHUQ7_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFR1135965.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1091..1828
                     /locus_tag="ACHUQ7_002611"
     CDS             1091..1828
                     /locus_tag="ACHUQ7_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFR1135966.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            1828..3924
                     /locus_tag="ACHUQ7_002612"
     CDS             1828..3924
                     /locus_tag="ACHUQ7_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFR1135967.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAHKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(3971..4249)
                     /locus_tag="ACHUQ7_002613"
     CDS             complement(3971..4249)
                     /locus_tag="ACHUQ7_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1135968.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4250..4393
                     /locus_tag="ACHUQ7_002614"
     CDS             4250..4393
                     /locus_tag="ACHUQ7_002614"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1135969.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            4519..4929
                     /gene="psiE"
                     /locus_tag="ACHUQ7_002615"
     CDS             4519..4929
                     /gene="psiE"
                     /locus_tag="ACHUQ7_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFR1135970.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(4973..6448)
                     /gene="xylE"
                     /locus_tag="ACHUQ7_002616"
     CDS             complement(4973..6448)
                     /gene="xylE"
                     /locus_tag="ACHUQ7_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HFR1135971.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKISAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGRRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQTESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQASGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(6819..7709)
                     /gene="malG"
                     /locus_tag="ACHUQ7_002617"
     CDS             complement(6819..7709)
                     /gene="malG"
                     /locus_tag="ACHUQ7_002617"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFR1135972.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(7724..9268)
                     /gene="malF"
                     /locus_tag="ACHUQ7_002618"
     CDS             complement(7724..9268)
                     /gene="malF"
                     /locus_tag="ACHUQ7_002618"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFR1135973.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(9422..10612)
                     /gene="malE"
                     /locus_tag="ACHUQ7_002619"
     CDS             complement(9422..10612)
                     /gene="malE"
                     /locus_tag="ACHUQ7_002619"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFR1135974.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSTGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            10977..12092
                     /gene="malK"
                     /locus_tag="ACHUQ7_002620"
     CDS             10977..12092
                     /gene="malK"
                     /locus_tag="ACHUQ7_002620"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFR1135975.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIYIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            12164..13504
                     /gene="lamB"
                     /locus_tag="ACHUQ7_002621"
     CDS             12164..13504
                     /gene="lamB"
                     /locus_tag="ACHUQ7_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFR1135976.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            13656..14576
                     /gene="malM"
                     /locus_tag="ACHUQ7_002622"
     CDS             13656..14576
                     /gene="malM"
                     /locus_tag="ACHUQ7_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFR1135977.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQELGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            14805..>14906
                     /locus_tag="ACHUQ7_002623"
     CDS             14805..>14906
                     /locus_tag="ACHUQ7_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1135978.1"
                     /translation="MIGHITNSGGNNSLMKHDVVQGNNIVDLDLLRNF"
BASE COUNT         3694 a         3832 c         3910 g         3470 t
ORIGIN      
        1 ttccccgcgc cgtaattcgc atgctttagt tgtgtatact cgatcccgcc cgaaatgttt
       61 ttgggtaaat ctccattcat tcaatgaagg gaaattgtta tgaaaaaagt tctgtatggc
      121 atttttgcca tatctgcgct tgcggcgact tctgcgtggg ctgcacctgt acaggtgggc
      181 gaagcggcag agtcggcagc aacgtcggtt tcggcgggga gttcctccgc gaccagcgtc
      241 agcaccgtaa gctcggcggt gggtgtcgcg ctcgcggcaa ccggtggcgg tgatggttct
      301 aataccggga ccaccacaac cacgaccacc agtacccagt aataaagtat gtatccccaa
      361 aataatttga gtcattgcat ctgtggcttg aagtatgaag ggatttacca taaccacact
      421 ccggtgtggt tattctgccc ctctggagaa gagtcgtgaa gcgacctgca ctcattctta
      481 tctgcctgct attacaggcc tgttcagcca cgactaaaga gctgggcaat tcactgtggg
      541 acagtctgtt cggcacgcca ggcgtacagc tgacggacga tgatattcaa aatatgccct
      601 acgccagcca gtacatgcag cttaatggcg ggccgcaatt gtttgtggtg ctggccttcg
      661 ctgaagacgg acaacaaaaa tgggtcactc aggatcaagc cactcttgtc acacaacatg
      721 gtcgtctggt gaagactttg cttggcggcg acaacctgat tgaagtgaat aaccttgccg
      781 ccgacccgct gattaaaccc gcacaaattg ttgatggtgc aacatggacg cgcacgatgg
      841 gctggaccga gtaccagcag gtacgctacg ccaccgcacg ctcagtcttc aaatgggatg
      901 gcaccgatac cgtcaaggtc ggcagcgatg aaaccctggt tcgcgtgctg gacgaagaag
      961 tctccaccga ccaggcgcgc tggcataacc gctactggat cgacagcgaa gggcaaattc
     1021 gccagtcgga acagtatctc ggcgcggatt atttcccggt gaaaaccact ctcatcaagg
     1081 cggcaaaaca atgattaaac aaactattgt cgcgttgctt ttgagtgtgg gagcgtcatc
     1141 ggtcttcgcg gcaggaaccg tcaaggtgtt cagcaatggc agcagtgagg ccaaaacgct
     1201 aacgggcgca gagcatttaa tcgatctggt agggcaacct cgactggcaa acagctggtg
     1261 gcctggtgcg gtgattagcg aagagctggc aacggcggca gcattgcgtc agcagcaggc
     1321 gttgctgaca cggctggcag aacagggcgc agattccagc accgacgatg ccgccgccat
     1381 taacgcctta cgccagcaaa tccaggcatt gaaagtgaca ggcaggcaaa aaatcaatct
     1441 tgatcccgat atcgtccgcg ttgccgaacg cggtaacccg ccgttgcagg gcaactacac
     1501 gctgtgggtc ggaccaccgc cgtccacggt cacgttgttc gggcttatca gccatcctgg
     1561 caatcagcca ttcactccag gtcgcgacgt ggcgagctat ctctctgacc agagcctgct
     1621 cagcggtgcg gatcgcagct acgcgtgggt cgtttacccg gacggacgca cgcaaaaagc
     1681 gccggtggct tactggaaca agcgtcacgt agagccgatg cccggcagca ttatttatgt
     1741 tggcctcgca gattccgtct ggagtgagac gcctgatgcc cttaacgccg acattcttca
     1801 gactctgacg cagcggatac cccaataatg aaaaaaagac atctgcttag cttactggcg
     1861 ctgggcatta gcacagcttg ctacggcgaa acatatcctg cgcccattgg tccgtcgcag
     1921 tcggatttcg gtggcgtagg attattacaa actcccaccg cgcgtatggc acgggaaggg
     1981 gagttgagtc tgaactatcg cgataacgat cagtaccgtt attactcagc ttcagtgcaa
     2041 ctcttcccgt ggctggaaac aacgctgcgc tacaccgacg tgcgcacccg gcagtacagc
     2101 agcgtcgaag cgttctctgg cgatcaaacg tataaagata aagccttcga tctcaaactg
     2161 cgtttgtggg aagagagtta ctggctgccg caagtggcgg ttggtgcgcg ggatatcggc
     2221 ggtacggggc tgtttgatgc ggaatatctt gttgccagca aagcctgggg gccgttcgat
     2281 tttacgctcg gcctgggctg gggatatctg ggcaccagcg gtaatgtaaa aaatccgctc
     2341 tgttcagcca gtgataaata ttgctatcgc gataacagct acaaacaggc gggatctatc
     2401 gacggtagcc agatgttcca cggtcctgcc tcactgtttg gcggcgtgga ataccagacg
     2461 ccctggcaac cgctgcgtct gaaactggag tatgaaggta ataattatca gcaggatttt
     2521 gccgggaagc tggagcaaaa aagtaagttt aacgtcgggg cgatttatcg cgttaccgat
     2581 tgggccgacg ttaaccttag ctatgaacgt ggcaacacct ttatgtttgg cgtcacgctg
     2641 cgcaccaact ttaacgatct gcgcccgtcc tacatcgata acgcccgccc gcaatatcaa
     2701 ccgcagccgc aggatgccat tttgcagcat tcggtggtgg cgaatcagtt aacgttgctg
     2761 aaatacaacg ccggacttgc cgatccgcag atccaggcga aaggcgatac gctgtatgtt
     2821 accggcgagc aggtgaaata tcgtgattcg cgcgaaggga tcatccgtgc taatcggatc
     2881 gtgatgaacg atctgccgga tgggatcaaa acgatccgca ttacggaaaa tcgccttaac
     2941 atgccgcaag tgacgacgga aaccgatgtc gccagcctga aaaatcatct cgccggagag
     3001 ccgttgggcc acgaaacgac gctggcgcac aaacgcgtcg agccagtggt tccgaagtcc
     3061 accgagcagg gctggtatat cgacaaatca cgctttgatt tccatatcga tccggtgctg
     3121 aaccagtcgg tcggtggccc ggaaaacttt tacatgtatc agctgggcgt gatgggaacg
     3181 gcagatttgt ggctgacgga ccatctactg accaccggca gcctgtttgc aaatcttgcc
     3241 aacaactacg acaagtttaa ctacaccaat ccaccgcagg actcgcactt accgcgcgtt
     3301 cgtacccatg tgcgcgagta tgtgcagaac gatgtctatg tgaataacct gcaagccaac
     3361 tacttccagc atctgggcaa cggcttctac ggtcaggtct atggaggtta tctcgaaacc
     3421 atgtttggcg gtgcgggggc cgaagtgttg tatcgtccgc tggacagtaa ctgggcgttt
     3481 ggtctggatg ccaactacgt taaacagcgc gactggcgta gtgcaaaaga tatgatgaaa
     3541 ttcaccgact acagcgtgaa aaccggacat ctgaccgcct actggacgcc atctttcgcc
     3601 caggacgtgt tggtgaaagc cagcgtcggg cagtatctgg caggggataa aggcggcacg
     3661 ctggagatcg ctaaacgctt tgacagcggc gtggtggtgg gtggctatgc cacgatcacc
     3721 aacgtttcga aagaggagta cggcgaaggg gacttcacca aaggcgtgta tgtctcggta
     3781 ccgttggatc tcttctcgtc tggcccgaca cgcagccgtg cggcgattgg ctggacgccg
     3841 ctgacgcgtg acggtggtca gcaacttggg cgtaagttcc agttgtatga catgaccagc
     3901 gaccgtagcg taaatttccg ctaagtcatg ggaaaggtgc cagttttcgc actcactggc
     3961 accctcattc ttaatgacct gttgagccat caggaacgca gttcaccccc ggtttacgat
     4021 ctggtgtgta aaggcgaaaa cattctgtct tcctgtccgt cgatatcggt tgcaggtctg
     4081 gttgcctgag cgactgggcc atcattgggc tggagacatt gctttgcggt aggtcggctt
     4141 tatcagcagt accgagcgga ccagcataag caggaagaac agagactgat aacatcaaag
     4201 cagcaaaata aggcttcatt tttaccacct ttatcaggtt acgtttcatt tgttccagag
     4261 gaacattgtc gatttttcgc gcattgctgg tggctgggaa tcacctgaat gggtgatttt
     4321 tgaattaccg gctttggtgc ggtttgtctt gccggatgcg ccgccaggcg cggcttatcc
     4381 ggcctacggg taggtatatc cggctttggt ggaggcgcgc tccaaatcca ggttgaacaa
     4441 aacatacaca aaaaatatag atctccgtca catttttgcg ttatacagga agctcgccac
     4501 tgtgaaggag gtactgctat gacgtcactc tctcgtccgc gcgtggagtt tatctccacc
     4561 attttgcaga ccgtactcaa tcttggcctg ctgtgcctcg gcctgatttt ggttgtcttc
     4621 ctcggcaaag aaacggtgca tctggctgat gtgctgttcg cgccagaaca aaccagcaaa
     4681 tatgagctgg tagaaggact ggtggtttac tttctctatt tcgaatttat cgcgctgatt
     4741 gtgaagtact ttcagtccgg ttttcacttc ccgctacgtt actttgtcta tattgggatc
     4801 accgccattg tgcgcttgat catcgtcgat cataaatcgc cactggatgt gttgatctac
     4861 tcggctgcga tcctgttact ggtgatcacc ctgtggctgt gtaattcgaa acggctgaag
     4921 cgggagtaaa aagacagcac gccgaaatgg cgcggcgtgc tggacaggaa gattacagcg
     4981 tagcagtttg ttgtgttttc ttcgtttccg gttcccagag cgcttccagc tcctcaaggg
     5041 ttttaccttt ggtttccggg acaaatttcc acataaacag tgctgccaga acgcccatac
     5101 aaccgtaaat ccagtaggag aaaccgttgt ggaaatgggc caccagccag gagtttttgt
     5161 ccatcatcgg gaaggtccag gagacgaagt agttcgccag ccactgggcc gccaccgcga
     5221 ttgccagcgc tttaccacga atagcattcg ggaagatttc cgacagcagt acccagcata
     5281 ccggacccca ggacatggca aaggcggcaa catagaacag catcgacagt agcgccacaa
     5341 tacccgatgc ctgagtgtaa aacgcggtac cgaggctaaa cataccgatt gccattccga
     5401 gtgcgccgat aatttgcagt ggcttacgac caaatttatc cacagtcata attgccagta
     5461 cggtaaaggt gaggttgata actccgacaa taatggtctg caacagcgcg atatccgtgc
     5521 tggcccccag cgttttgaac acttccggcg cgtagtacag caccacattg atgccgacaa
     5581 attgctggaa gatggagagc ataacgccga ttacaatcac gcccacgcca aacatcagca
     5641 gacgaccacc ggttttgcgg ccatgatcca gggagtgttt aatttcctgt actgcctgag
     5701 ttgcaagcgt gttgcccata attttgcgca ggatactttc cgtctgttct tgcttgccgc
     5761 gtgacatcag ccagcgagga ctttctggca cggtatacag cagcattaag aacagcagtg
     5821 cagggataca ttccgaggca aacatataac gcctgccgtc agtattcagc cagctggcat
     5881 caccggaacg ggcaataaaa tagtttacgc agtaaactaa aagttgcccg aaaataatcg
     5941 caaactggtt aaatgaaacc agttttccgc gaatatgagc tggagccagt tccgcaatat
     6001 acattggtga aagcattgag gctaaaccaa cgccaatacc gccaataata cgataaataa
     6061 caaattccgg gacataacct gccagataaa taggtactgt gttgtccggg tttatagagg
     6121 taaaaccaag ttctggccag gcagaaccta caccagaaat aaaaaacagg acagcagaaa
     6181 tcttaagtga atcacgacgg ccgaagcggt tactgcaata accaccgagg gcaccgccga
     6241 tgatgcaacc aatcagagcg ctggctacgc aaaatcctaa cagggagttg gcagcggatt
     6301 cacttaagtt ttgtggagca acaaagacgg tattgagtga ctcaacagta ccggaaataa
     6361 cggcggtgtc gtagccaaat aataaaccac ctaatgtagc gactaaggta atcgaaaata
     6421 tataactgga attatactgg gtattcattc agacctgcct tagaccattc tgatctttta
     6481 tcagtgcatt gatgttggaa ttgtcaggca gttaaggcaa ttatcatttt ttacaatgcg
     6541 tttacgtaat gtcttttctg tgatcttaat tgtgataatt atcaaaaatt gatgaaacaa
     6601 tactattgcc gtgactcaga gcacgaaaga gaattatcgt aagtgggaaa acaaataacg
     6661 taaaaataat aagttctatg atgggaatat taaccggcga acgattcaga ttgcagacga
     6721 aagaaaaaaa ggcgctccgt ggagcgccga ataatagtca caagttggga taacgtaagt
     6781 tgagggtgca gcggcataac attggcagaa caacatcttt aacctttcac accacctgcc
     6841 gtcaggccgt tgaccagcca gcgttgagcc agcaagaaga cgatggtgat cggtaatgca
     6901 gacatcacgg cagcggcggc aaagtcaccc cacaggtagt tttgcgggtt gaggtattgc
     6961 tgcatcccca cggccagggt gtagctgttt acgtcacgca gtaacagcga cgcgaccgga
     7021 acttcagtaa tggcagcgat aaacgacagg atgaatacca ccgccagaat cggtactgac
     7081 agcggcaaca ggacaaggcg gaaggcctgc cacggtgtcg caccatccag cgcagccgct
     7141 tcttccagcg aactgtcgat ggtttcgaaa tagcctttga tggtccagac atgcagcgca
     7201 atcccaccca gatacgcgaa aattacgccg ccgtgagtat tcaggccaat gaatggaatg
     7261 tactcaccca gacgatcaaa caacgcatac aacgcgacca gtgaaagtac tgccgggaac
     7321 atctggaaaa tcagcattcc tttcagcagc gtcgctttgc ctggaaagcg catacgggcg
     7381 aaagcgtagg cgcaggtggt ggagagcgcc acaatgccaa tcgcggaaat cccagcgacc
     7441 tttaccgagt tccacagcca cagcagtacc gggaatggcg gtggcgtaat gcgaccatca
     7501 gcctgttcaa cgctaaaacc taacgccagt ttccagtgat cccaggagat ttgctccggg
     7561 atcaggctac cggtcgcgaa gtttccctga cgcagcgaga tagcgacgac catcagcagc
     7621 gggaacataa tcgccgcgat aaaaagtagc agtagcaggt gagtaataaa caaacgtgct
     7681 ttttgcgatt tcggttggac cattgccatt tttgttatct cccttaatca aacttcattc
     7741 gcgtggcttt caggttcact atcgccagcg cacccaccag caggaagatc agcgtggcaa
     7801 ttgctgccgc cagaccgaag tcctgacccc cgccgccttc aaaagcgata cggtaggtgt
     7861 agttaacaag caggtcggta taaccggctg gcgtggtcgt gccaagacga tccgggccgc
     7921 cgttggttaa cagctgaatc agcacgaagt tgttaaagtt aaaggcgaag ctggcgatca
     7981 tcagcggcgt cagcggttta atcagcagcg gcagcgtaat cttaaagaag ttctggaacg
     8041 gacctgcgcc atccattgct gaggcttcat acaaatcgtc cggaatcgct ttcagcaagc
     8101 ccatgcagag gatcatcatg tacggataac ccagccaggt attgacgata atcagcatcg
     8161 tgcgagcggt ggtcggatcg ctgaaccagg cgggcttcac gccaaacagc gcgctcaaca
     8221 tcatgttgat ttcaccgaag ctctggttaa acaatccttt gaaaatcaag attgaaatga
     8281 acgatggcac cgcgtagggc agaatcagca ggacgcgata gaccgctttg ccgcgcaacg
     8341 cttcccactg caccagacat gccagaacca tgccgaccgc caccgttaaa aagacagtga
     8401 tcagcgagaa caccacggtc cagacgaaaa tggcgaggaa cggtttctga atgccttcgt
     8461 cggtaaagac gcgggtaaag tttttccagc cggtggtcac ggtgtaaccg gggcttagct
     8521 tttcatcccc ccagttgccg tcggcggtaa tggactggta aaagccaatt tggttattcg
     8581 gacgatattt cacgccactc tgattattcg tcaacgtgcc gtcaccgtcg agtgtgtaga
     8641 gcggctgcgt gccagaaaac tggcgcaggg agctcatcat cactttgttg ccatccggca
     8701 gaatggcggt aatgtcactc agcgcctgac gattctgggt aatcacgcgc agattcgcgc
     8761 gttcgccttc gggctgggcg gtcgtttctt tcagttgcag tttttgctcg ccgccaaatt
     8821 taaacgcgtc ggagaggtaa tttttgccgg tttcgccgtc gctgagcgcc agttgccact
     8881 catcgcccgc cgggtaaaga ccaaagttat aggttttgcc tgcttgccag gagcgatcta
     8941 acaacacttc ctgcgcacgt tcaaaagtca gctggttagt gctgctgtag ttggtgaagg
     9001 caatggcgat ggtgcagacc agagggaaga ggacgaataa tcccattcca gccattcccg
     9061 ggtaaacata gcgccaggcg taggctttac gattggcgaa aatatacagc cccgctgaac
     9121 tcaatatcag cgtggtaatg gcgaacaggt attccccttg tgcgtacatt aaaacaacaa
     9181 ggtaacccac cagcaggccg agcagaccta gcactgacca tttcagcgcg tcgctttgcc
     9241 accaatgttt ctttttaatg acatccatgg ggttcttcct cattccagga cggataaggc
     9301 tttcacgcct tatccgacaa caactgcctg atgcgacact gatgcgtctt atcaggccta
     9361 catgcgtttc agttttgtag gccggacaag gcgttcacgc cgcatccggc atttcacagc
     9421 attacttggt gatacgagtc tgcgcgtctt tcagggcttc atcgacagtc tgacgaccgc
     9481 tggcggcgtt gatcaccgca gtacgtacgg cataccagaa agcggacatc tgcgggatgt
     9541 tcggcatgat ttcacctttc tgggcgtttt ccatggtggc ggcaatacgt ggatctttcg
     9601 ccaactcttc ctcgtaagac ttcagcgcta cggcacccag cggtttgtct ttattaaccg
     9661 cttccagacc ttcatcagtc agcagatagt tttcgaggaa ctctttcgcc agctctttgt
     9721 tcggactggc ggcgttaata ccggtgctca gcacgccaac gaacggttta gatggttgac
     9781 ccttgaaggt cggcagtacc gttacaccat aattcacttt gctggtgtcg atgttggacc
     9841 atgcccacgg gccgttgatg gtcatcgctg tttcgccttt attaaaggca gcttctgcga
     9901 tggagtaatc ggtgtctgca ttcatgtgtt tgtttttaat caggtcaacc aggaaggtca
     9961 gacccgcttt cgcgccagcg ttatccacgc ccacgtcttt aatgtcgtac ttgccgtttt
    10021 catacttgaa cgcataaccc ccgtcagcag caatcagcgg ccaggtgaag tacggttctt
    10081 gcaggttgaa catcagcgcg ctcttacctt tcgctttcag ttctttatcc agcgccggga
    10141 tctcttccca ggtttttggc gggttcggca gcaggtcttt gttataaatc agcgataacg
    10201 cttcaacagc gattgggtaa gcaatcagct tgccgttgta acgtacggca tcccatgtaa
    10261 acggatacag cttgtcctgg aacgctttgt ccggggtgat ttcagccaac aggccagatt
    10321 gagcgtagcc accaaagcgg tcgtgcgccc agaagataat gtcagggcca tcgccagttg
    10381 ccgcaacctg agggaatttc tcttccagtt tatccggatg ctcaacggtg actttaattc
    10441 cggtatcttt ctcgaatttc ttaccgactt cagcgagacc gttatagcct ttatcgccgt
    10501 taatccagat taccagttta ccttcttcga ttttggcgag agccgaggcg gaaaacatca
    10561 tcgtcgttaa tgcggataat gcgaggatgc gtgcacctgt ttttattttc ataatctatg
    10621 gtccttgttg gtgaagtgct cgtgaaaaca cctaaacgga ctctagtttc tttatacggc
    10681 aacctctttc catcctcctt gcccctacgc cccaccgttg ctttgtgtga tctctgttac
    10741 agaattggcg gtaatgtggg gatgcgcaca taaaatcgcc acgatttttg caagcaacat
    10801 cacgaaattc cttacatgac ctcggtttag ttcacagaag ccgcgttctc atcctcccgc
    10861 ctcctccccc ataaaaaagc cagggggtgg aggatttaag ccatctcctg atgacgcata
    10921 gtcagcccat catgaatgtt gctgtcgatg acaggttgtt acaaagggag aagggcatgg
    10981 cgagcgtaca gctgcaaaat gtaacgaaag cctggggcga ggtcgtggta tcgaaagata
    11041 tcaatctcga tatccatgaa ggtgaattcg tggtgtttgt cggaccgtct ggctgcggta
    11101 aatcgacttt actgcgcatg attgccgggc ttgagacgat caccagcggc gacctgttca
    11161 tcggtgagaa acggatgaat gacactccgc cagcagaacg cggcgttggt atggtgtttc
    11221 agtcttacgc gctctatccc cacctgtcag tagcagaaaa catgtcattt ggcctgaaac
    11281 tggctggcgc aaaaaaagag gtgattaacc aacgcgtcaa ccaggtggcg gaagtgctgc
    11341 aactggcgca tttgctggat cgcaaaccga aagcgctctc cggtggtcag cgtcagcgcg
    11401 tggcgattgg ccgtacgctg gtggccgagc caagcgtatt tttgctcgat gaaccgctct
    11461 ccaacctcga tgctgcactg cgtgtgcaaa tgcgtatcga aatctcccgt ctgcataaac
    11521 gcctgggccg cacaatgatt tacgtcaccc acgatcaggt cgaagcgatg acgctggccg
    11581 acaaaatcgt ggtgctggac gccggtcgcg tggcacaggt tgggaaaccg ctggaactgt
    11641 accactatcc ggcagaccgt tttgtcgccg gatttatcgg ttcgccaaag atgaacttcc
    11701 tgccggtaaa agtgaccgcc accgcaatcg atcaagtgca ggtggagctg ccgatgccaa
    11761 atcgtcaaca agtctggctg cctgttgaaa gccgtgatgt ccaggttgga gccaatatgt
    11821 cgctgggtat tcgcccggaa catctactgc cgagtgatat cgctgacgtc atccttgagg
    11881 gtgaagttca ggtcgtcgag caactcggca acgaaaccca aatctatatc cagatccctt
    11941 ccattcgtca aaacctggtg taccgccaga acgacgtggt gttggtagaa gaaggtgcca
    12001 cattcgctat cggcctgccg ccagagcgtt gccatctgtt ccgtgaggat ggcactgcat
    12061 gtcgtcgact gcataaggag ccgggcgttt aagcacccca caaaacacac aaagcctgtc
    12121 acaggtgatg tgaaaaaaga aaagcaatga ctcaggagat agaatgatga ttactctgcg
    12181 caaacttcct ctggcggttg ccgtcgcagc gggcgtaatg tctgctcagg caatggctgt
    12241 tgatttccac ggctatgcac gttccggtat tggctggaca ggtagcggcg gtgaacaaca
    12301 gtgtttccag actaccggtg ctcaaagtaa ataccgtctt ggcaacgaat gtgaaactta
    12361 tgctgaatta aaattgggtc aggaagtgtg gaaagagggc gataagagct tctatttcga
    12421 cactaacgtg gcctattccg tcgcgcaaca gaatgactgg gaagctaccg atccggcctt
    12481 ccgtgaagca aacgtgcagg gaaaaaacct gatcgaatgg ctgccaggtt ccaccatctg
    12541 ggcaggtaag cgcttctacc aacgtcatga cgttcatatg atcgacttct actactggga
    12601 tatttctggt cctggtgccg gtctggaaaa catcgatgtt ggcttcggta aactctctct
    12661 ggcagcaacc cgctcctctg aagctggtgg ttcttcctct tttgccagca acaatattta
    12721 tgactatacc aacgaaaccg cgaacgacgt tttcgatgtg cgtttagcgc agatggaaat
    12781 caacccgggc ggcacattag aactgggtgt cgactacggt cgtgccaacc tgcgtgataa
    12841 ctatcgtctg gttgatggcg catcgaaaga cggctggtta ttcactgctg aacatactca
    12901 gagtgtcctg aagggcttta acaagtttgt tgttcagtac gctactgact cgatgacctc
    12961 gcagggtaaa ggtctgtcgc agggttctgg cgtcgcgttt gataacgaaa aatttgccta
    13021 caatatcaac aacaacggtc acatgctgcg tatcctcgac cacggtgcga tctccatggg
    13081 cgacaactgg gacatgatgt acgtgggtat gtaccaggat atcaactggg ataacgacaa
    13141 cggcaccaag tggtggaccg ttggtattcg cccgatgtac aagtggacgc caatcatgag
    13201 caccgtgatg gaaatcggct acgacaacgt cgaatcccag cgcaccggcg acaagaacaa
    13261 tcagtacaaa attaccctcg cacaacaatg gcaggctggc gacagcatct ggtcacgccc
    13321 ggctattcgt gtcttcgcaa cctacgccaa gtgggatgag aaatggggtt acgactacaa
    13381 cggcgatagc aaggttaacc cgaactacgg caaagccgtt cctgctgatt tcaacggcgg
    13441 cagcttcggt cgtggcgaca gcgacgagtg gaccttcggt gcccagatgg aaatctggtg
    13501 gtaatagcaa aacctgggcc ggataaggcg tttacgccgc atccggcata aaaacaggtt
    13561 gtcattatct gaaaggggcg aaagcccctc tgattatcgg gtttagcgcg ctattgcctg
    13621 gctaccgctg agctccagat tttgaggtga aaacaatgaa aatgaataaa agtctcatcg
    13681 ccctctgttt atcagcaggg ttactggcaa gcgcgcctgg aattagcctt gccgatgtta
    13741 actacgtacc gcaaaacacc agcgacgcgc cagccattcc atctgccgcg ctgcaacaac
    13801 tcacctggac accggtcgat caatctaaaa cccagaccac ccaactggcg accggcggcc
    13861 aacaactgaa cgttcccggc atcagtggtc cggttgctgc gtacagcgtc ccggcaaaca
    13921 ttggcgaact gaccctgacg ctgaccagcg aagtgaacaa acaaaccagc gtttttgcgc
    13981 cgaacgtgct gattcttgat cagaacatga ccccatcagc cttcttcccc agcagttatt
    14041 tcacctacca ggaactaggc gtgatgagtg cagatcggct ggaaggcgtt atgcgcctga
    14101 caccggcgtt ggggcagcaa aaactttatg ttctggtctt taccacggaa aaagatctcc
    14161 agcagacgac ccaactgctc gacccggcta aagcctatgc caaaggcgtc ggtaactcga
    14221 tcccggatat tcccgatccg gttgctcgtc ataccagcga tggcttactg aaactgaaag
    14281 tcaaaacgaa ctccagctcc agcgtgttgg taggaccgct atttggttct tccgctccag
    14341 ctccggttac ggtaggcaac acggcggcac cagctgtggc tgcacctgct ccggtgccgg
    14401 tgaagaaaag cgagccgatg ctcaacgaca cggaaagtta ttttaatacc gcgatcaaaa
    14461 acgctgtcgc gaaaggtgat gttgataagg cgttaaaact gcttgatgaa gctgaacgcc
    14521 tgggatcgac atctgcccgt tccaccttta tcagcagtgt aaaaggcaag gggtaattac
    14581 gccccacagt gctgattttg caacaactgg tgcgtctcct ggcgcacctt tttttatgct
    14641 tccttcctgg gatatgagcg attttttata gtacttcact tcttcttcac taagaatgtc
    14701 cattatttca atgcattatc agagattctt ttcctttcgc cggtagtgtc tggacgctca
    14761 ggctactttt ccaggttatt ttatttctgt attacagagg ttttatgata ggtcatatca
    14821 caaattctgg cggcaataac tctttgatga aacatgatgt ggtgcaagga aataatatag
    14881 tagatcttga tttactacgt aatttc
//
LOCUS       DAZPCS010000089        14694 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZPCS010000089 DAZPCS010000000
VERSION     DAZPCS010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14694
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.089"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..1260)
                     /locus_tag="ACHUQ7_002624"
     CDS             complement(<1..1260)
                     /locus_tag="ACHUQ7_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1135979.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGIDPCEQKRVKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALELSRLPELLSRIDG
                     YKGQPVTRLAVMLNLLVFIRSSELRYARWSEIDIDNSMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQVVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TQEVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANREKGISPFEYAKINNPLK"
     gene            complement(1458..1533)
                     /locus_tag="ACHUQ7_002625"
     tRNA            complement(1458..1533)
                     /locus_tag="ACHUQ7_002625"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1498..1500),aa:Phe,seq:gaa)
     gene            complement(1639..2346)
                     /locus_tag="ACHUQ7_002626"
     CDS             complement(1639..2346)
                     /locus_tag="ACHUQ7_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFR1135980.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLITPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            2499..2582
                     /gene="yqgH"
                     /locus_tag="ACHUQ7_002627"
     CDS             2499..2582
                     /gene="yqgH"
                     /locus_tag="ACHUQ7_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgH"
                     /protein_id="HFR1135981.1"
                     /translation="MHAISLHSLAYRRFARNVSLSMLLLMR"
     gene            2744..4879
                     /gene="speC"
                     /locus_tag="ACHUQ7_002628"
     CDS             2744..4879
                     /gene="speC"
                     /locus_tag="ACHUQ7_002628"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFR1135982.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LTIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFISMMADSSPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSSLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(4928..6184)
                     /gene="nupG"
                     /locus_tag="ACHUQ7_002629"
     CDS             complement(4928..6184)
                     /gene="nupG"
                     /locus_tag="ACHUQ7_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFR1135983.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(6386..7465)
                     /gene="mltC"
                     /locus_tag="ACHUQ7_002630"
     CDS             complement(6386..7465)
                     /gene="mltC"
                     /locus_tag="ACHUQ7_002630"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFR1135984.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(7530..7805)
                     /locus_tag="ACHUQ7_002631"
     CDS             complement(7530..7805)
                     /locus_tag="ACHUQ7_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFR1135985.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(7833..8885)
                     /gene="mutY"
                     /locus_tag="ACHUQ7_002632"
     CDS             complement(7833..8885)
                     /gene="mutY"
                     /locus_tag="ACHUQ7_002632"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFR1135986.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            9046..9765
                     /gene="trmB"
                     /locus_tag="ACHUQ7_002633"
     CDS             9046..9765
                     /gene="trmB"
                     /locus_tag="ACHUQ7_002633"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFR1135987.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            9765..10091
                     /locus_tag="ACHUQ7_002634"
     CDS             9765..10091
                     /locus_tag="ACHUQ7_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFR1135988.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            10275..10994
                     /locus_tag="ACHUQ7_002635"
     CDS             10275..10994
                     /locus_tag="ACHUQ7_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFR1135989.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            11170..12216
                     /gene="ansB"
                     /locus_tag="ACHUQ7_002636"
     CDS             11170..12216
                     /gene="ansB"
                     /locus_tag="ACHUQ7_002636"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFR1135990.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINADCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            12333..13295
                     /locus_tag="ACHUQ7_002637"
     CDS             12333..13295
                     /locus_tag="ACHUQ7_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFR1135991.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAISLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANER"
     gene            complement(13396..>14694)
                     /locus_tag="ACHUQ7_002638"
     CDS             complement(13396..>14694)
                     /locus_tag="ACHUQ7_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFR1135992.1"
                     /translation="DSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIAA
                     ITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVNV
                     LANMMFGSISGSAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNVL
                     IVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKAF
                     LDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRHLPKLILES
                     VVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGIF
                     MDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISGV
                     KIQHLIKPLLLFYAALLVALMMITYIPQISLFIPQLLGLM"
BASE COUNT         3833 a         3547 c         3789 g         3525 t
ORIGIN      
        1 ttttaatgga ttgttaatct ttgcatattc aaacgggctg atacccttct cccggttcgc
       61 atctagaaaa tcggcccacc attgcaacat cagtcgccgt tcttccagat gttctgcttt
      121 atggatatac gcagcacgta caccattacg ctcctgatgg ctcatctgac gttccacagc
      181 atcgcgggac cacaaacctg attcaatcaa tgcactgcac gccatcgccc ggaatccatg
      241 gccacagact tcctgggttg tgtcataccc catcaccctc agggctttat ttacagtatt
      301 ttcactgatt ggtttacgcg ggtcatgtgc acccgtaaag atcagaccat tttcaccagc
      361 ccatgtctga agttctgcca gtatggctac aacctgtttg ctgagtggca caagatgtgg
      421 tgttcgcatc ttggatcccc ggtgtgagaa ttttacgcct ggcagaggtt cgcgttcggc
      481 tggaatagtc cacatggaat tgtcaatatc aatttccgac cagcgggcat atctgagttc
      541 actggaacga ataaaaacca gtaaattcag cataactgcc agtcgggtga caggctggcc
      601 tttataaccg tcaatacgcg acagaagttc aggcaggcgt gaaagctcca gagcagggcg
      661 atgctggcgt tttaccgtgg tcaatgcacc agccatatcc agagccgggt tatatctgat
      721 gatgccagac tgtacggcgt aacgcattac agcagatatt ctttgctgca gacgactggc
      781 gatttcataa atttgttttg cttctgcagc acgaacaggg ataagcaggt ccggggtatt
      841 gagtgttgtg atatcccggt tgccgataaa ggggaaaacg tgtgtctcga ggcttttgag
      901 tactttatcg ctgtgtgatt gtgcccattg tttattactg gcatgccagc gtcgtgcaat
      961 atgttcaaaa gagagctgta aatcagggac aactttttta actcttttct gttcgcaagg
     1021 gtcaatcccc tctgaaatca gttttcgggc ctcatcccgt tttgtacgag cttcggagag
     1081 agaaacttct ggatacacac caagtgccag agtcttctcc ttacccagaa tacgataacg
     1141 gagacgccag taacgggaac cattaggatg aaccagcagg aacatgccgg ccccgtcagt
     1201 gagtttataa gccttgtcag tgggctttgc agcccggatt tttgcgtcag tcagtgccat
     1261 agttcagcct tctgaatgcg ttaaagaggg tacaaattct gtcgaactgg ggtatacccg
     1321 caattatacc cacacaatgg ggttgatgtg gggggaatct ggttgagttc ggtagaattg
     1381 atttggagga agaacactta aatcatggtg ttctgaaacg aaaaacggac ctccgtggag
     1441 gtccgttgat atgaatttgg tgcccggact cggaatcgaa ccaaggacac ggggattttc
     1501 aatcccctgc tctaccgact gagctatccg ggcaacgggg cgcattaaac ctgattcgcc
     1561 tcgtctcgtc aatcaaattt cttcaattta ctgcagactg cacaatctat catcactttg
     1621 ccgctattgc acgcattgtc aggcaaacca ggcggtccag gcggcggaaa gcggcattgc
     1681 cagaaggagt gctggaagca tgttgaccac cgggaacatt ttaatgccac agatgcgtaa
     1741 tccggttgcc agcagcaata aaccgcctac ggcgctgaag tctgccatca tcgacggtgt
     1801 gatcagcggt aatatcagcg cggcagccca cgccagcgtt aactggatga tcagtaatgg
     1861 gatactaatt accgacaccg caatacccag cgagcaggcg aagatcatcg ccgtaaagaa
     1921 atcaagaaat gacttggcga ttaaaatact cggatcgccg gtcatccctt cgttcatcgc
     1981 cccgaagatc ccggtgccgc tggcgcaaaa caggacaata atcgcgacat aattctgaat
     2041 aaaagattca tgcgctggct tcttacgtga gtgacgaaac agattttgtg ctttggcgac
     2101 tgctgtattg acacctttct ccagcaaaca aatttcgcca attagagcgc caagtagcgt
     2161 agctaaaacc atcgctggaa ggttggcgca tttcacaacc agtaaaatac caatccccag
     2221 cgatgccaga ccaaaaatag atgtcatgga gacgcggata cgttccggta agcgttggct
     2281 gagcagtgcg ccgagaacgc cacccagtaa gactgcacta gcgttgataa aagggccgat
     2341 gaccacaaga gttcctgtta gctgtattgt ttgattttct tattatgaac tttttgcctt
     2401 gttgatggct tgtctggtcg caaagtcctg gcttgcacgc cttagcgaaa ggtgccatga
     2461 ttgcgcgaat tttctcctct ctgtacggag tttgcccgat gcacgccatc tccttacatt
     2521 ctctcgctta tcgccgtttc gcgcgaaacg tttccctttc tatgttactg ctcatgcggt
     2581 gaagttaacg cacgctcact gcaggacaac agtaaaatca gagcgtttct gcttttactg
     2641 atgtctggcg gtcggagctg gtgaccagtt tgacccatat ctcatggggc agggttttcc
     2701 accttgtccg gtattcttac ttccccgaaa cgggtttgcg cttatgaaat caatgaatat
     2761 tgccgccagt agtgaactgg tatcccgact ttcttctcat cgccgcgtgg tggcgttggg
     2821 agatactgat tttacggacg tcgcggcagt cgtcattacc gctgcggata gtcgcagtgg
     2881 cattcttgcg ttgcttaagc gcaccggttt tcatctaccg gtgtttttgt attccgaaca
     2941 tgctgttgaa ttacctgcgg gcgttacggc ggtaatcaac ggcaacgagc agcagtggct
     3001 ggagctggaa tccgcagcct gtcagtatga agagaatttg ctgccaccgt tttatgacac
     3061 gctgacgcag tacgttgaga tgggcaacag cacctttgct tgccctggac atcaacatgg
     3121 tgcgttcttt aaaaagcatc ctgccggacg ccatttttac gatttctttg gtgagaacgt
     3181 ctttcgcgcc gatatgtgta acgctgacgt aaaattgggc gatctgctta ttcatgaagg
     3241 atcggcgaaa gatgcgcaga aattcgcagc caaagtcttt catgccgata aaacctattt
     3301 tgtgctgaac ggcacatcgg cagcgaataa agtggtgacg aatgcgctgt taacgcgtgg
     3361 cgatctggtg ctcttcgacc gtaacaacca taagtcgaat catcacggcg cgctgattca
     3421 ggcggggacg acgccggtct atctggaagc ttcacgtaac ccgtttggtt tcattggcgg
     3481 tattgatgcg cactgtttta atgaagagta tctgcgccag caaattcgcg acgttgcgcc
     3541 agaaaaagcc gacctgccgc gcccgtttcg cctgacgatt attcagctgg gaacctatga
     3601 cggcactgtc tataacgccc gtcaggtgat cgataccatt gggcatctgt gtgattacat
     3661 tctgtttgat tccgcgtggg tcggttacga acagtttatc tcgatgatgg cggatagctc
     3721 gccgctgctg ttagaactta acgaaaacga tccggggatt tttgtgaccc agttggtgca
     3781 caaacagcag gctggattct cacagacgtc gcagatccat aaaaaagata accatatccg
     3841 cggacaggcg cgtttttgcc cgcataagcg gttgaataac gcctttatgc tccatgcttc
     3901 taccagccct ttctatccgc tgtttgctgc actggatgtt aacgccaaaa ttcatgaagg
     3961 ggagagtggg cgtcggctgt gggctgagtg cgttgagttg ggtattgagt cgcgcaaggc
     4021 tattcttgcg cgctgtaagc tgttccgccc gtttatcccg cccgttgttg atggcaaatt
     4081 gtggcaggat tatccgacat cagtgttagc cagcgaccgc cgttttttca gttttgagcc
     4141 gggggcgaag tggcacggct ttgaaggata tgccgcggat cagtattttg ttgatccgtg
     4201 caagctgtta ctcactacac caggtatcaa tgccgaaacc ggcgaatata gcgactttgg
     4261 cgttccggcg acgattctgg cgcactatct gcgtgagaac ggcattgtgc cggagaagtg
     4321 cgatctcaac tccattctgt tcttattaac tccggcggaa agccacgaga agctggcaca
     4381 actggtggcg atgctggcgc aatttgaaca gcatattgag gatgactcgt cgctggctga
     4441 ggtgttgccg agcgtttata acaaatatcc ggtgcgctat cgcgactaca ccctgcgcca
     4501 gttgtgtcag gagatgcacg atctgtatgt cagtttcgac gtcaaagacc tacaaaaagc
     4561 gatgttccgc cagcagagtt tcccgtcagt ggtgatgaac ccccaggatg cgcatagcgc
     4621 ttatattcgc ggtgaagtgg agttggtgcg gattcgtgat gccgaagggc gaattgcggc
     4681 agaaggggcg ttgccttatc cacctggcgt gctttgcgtg gtacccgggg aagtctgggg
     4741 tggggcggtt caacgttatt tccttgcgct ggaagaaggc gtgaatttgt tgccgggatt
     4801 ttcgccggag ttgcaaggtg tctatagcga aaccgatgcg gatggcatga aacgattgta
     4861 cggttatgtg ttgaagtaag aataaaaaaa cgggtcacct tctggcgacc cgtttttctt
     4921 tgcgtaatta gtggctaacc gtctgtgtgc ctgtcgggac acgaacgtgt ttatatttga
     4981 acatcgccat gaacgcgaag gccagaacca cggaataacc agcgaaaatc aaccatacgg
     5041 tctgccagtc ggtaatgccg ttttgggtgt acatctcaac aactttaccg ctcacgatgc
     5101 cgccgaggat acagccgaag ccgttagtca tcatcaggaa catcccttgt gcactggcgc
     5161 gaattgccgg gctaacttct ttttcgacaa acaccgaacc agagatgttg aagaagtcga
     5221 atgcgcaacc gtaaacgatc atcgacagta ccagcagtac agtaccgaac ggagtcgggt
     5281 cgccgtaagc aaacagcgca aaacgcagga tccacgccac aatactgatc atcattacgt
     5341 tcttaatacc gtagcggctt aagaagaacg ggatggtcag aatgaacagg gtttcagaga
     5401 tctgcgaaat cgacatgatg attgacgcat gctgcacgat gaagctgctg gcaaacatcg
     5461 gatctttgtc gaagctgtgc aggaaggtat taccgaacat gttggtaatc tgcagttccg
     5521 cgcccagcag cattgagaag atgaagaaga ttgccatacg cttgttttta aacagcgcga
     5581 atgcatcgag gcccagcagg gttgtccagc tctgattcgc ttgctgttta gcaaccggaa
     5641 tatgcggcag agtcagggta aacagaacca gaatggcgga aagtgctgcg ccaatataca
     5701 gctgcatgtg gcttaattcg aagccagaca ggctcaccac ccacattgcc atgataaagc
     5761 cgatggtgcc ccagatacgg attggcggga agtcagtaac gatatccatc ccggcatttt
     5821 gcaggcgata gtaagagatg gtgttgatta acccaagcgt tggcatataa gcaaacgagt
     5881 taatcaatat cacaaggaac atcgcttccg gcgtcgtgac ctgtgccgcc atgaacagcg
     5941 tgatagcgcc aatggtgtgg caaatggcat atacccattt cgcacttaac catttgtcgg
     6001 ccacaatccc cagcagcgca ggcataaata ccgcggcgat acccagtgag ctataaactg
     6061 cgccaataga agcaccgtca aacttcaggg taacaaacat ataggagccg agggtcgtca
     6121 gccaacttcc ccacagacag aactgcagaa aagagaggat tttcagctgc agcttaagat
     6181 tcatgttaat ttcctcacat cgtgatgcgg atgagtgttt gaaaaagcac atttgaccgt
     6241 gcgagatgat gggattctat caacttcgcg gactggtttt ttgttacctg tggcaaataa
     6301 ttgcaaacat tcccaatttg caaaacgaaa ttgtgatgtg gataacattt ttgcccctga
     6361 gcatcgtcag gggcggttaa tggaattatc ggcggcggta ggatttttgc gcggtattca
     6421 ctttataaag ataacggcga gattccgcag agggatggcg ggtcgtcagc gtctgataaa
     6481 catcgcccgg cgtcatggtg ttaataatat tggcagcctg aatcttatca ttcgaaaaga
     6541 ctcgcagcac gctgcctgcg ccgccgttat aggcggtgat gacggcataa cgccgcgatg
     6601 ttgggttatc aattccgccg agataaacat tgttcagcat cgccagatac gcggtgccgg
     6661 tatcaatatt gctggcagga tcaaacaaga aactgcggct cggcgtgccg gatttcccct
     6721 gcgagcggaa cacatctttc ccggcagtat tttgtaccac ctgcattaat cccagcgcat
     6781 cggaacggct gaccgcatac gggttaaagg aagattcagt ctgcataatt gccagaatca
     6841 gcgactcatc aacgccatat ttccgtgacg cctggcggac catgccgaga tatttgtgcg
     6901 cacgtttatc aaggtggttc ggcaccatgt taatggtgac gctgtagatg atacgtagcc
     6961 cgttgctgcg gcttttcaga cggtttttca gcagataatc cgcgaagttg cttgcgcgac
     7021 cttcccagcg aatcggctgc ccggtgttgt ccaccacctg accgtaaagg aaaggttctt
     7081 tcgaaattgt aatatcatca acgtcggaat agagatcgac cgaactcgga tcgtcaccca
     7141 tcagcaacgt tttgataatt gcccggcgca aatgcgcggc aggttctgtc ccggcgatgg
     7201 tttcgatagt aatcgtaccg tcatcgaagt tgatgtggct gcgggtctga tattgatcgg
     7261 tgtatttcac gtagtcctta ggaccggcga tcaccacctc tttgaagccc cagatgttct
     7321 caatattgtg ggcaaattgc cccatcagaa tatcaaaacc gttggtatct ttgacccagg
     7381 cttcgttata ggtatcgcct tttttggtcg tcgaacagga gatgagcaac ggcgcaatca
     7441 aagccagcgc gagatatttt ttcatcattc cgggtgcgtg ttgtgtttgt ttttatgcaa
     7501 gctgccggag gcgtgctccg gcactgtttt tattttttat cttccggcgt atagccctcg
     7561 atatgcacct ctttaccctc gaacaggaag ttgaccatct cctgctccag cagcttgcgg
     7621 tgctcggcat tcatcatgtt gagtttcttt tcattaatca gcatggtttg tttgtgctgc
     7681 cactgcgccc aggcttcttt ggagatctcg ttatagatgc gttttcccag ctcgccgggg
     7741 tacagctgaa aatcctgacc ttctgcttca cgttgcagga aagtacaaaa aatcgttctg
     7801 ctcataaatc atcctcttta tcgactcacg cgctaaaccg gcgcgccagt gcgtaactgc
     7861 tgtaacaaac gctccacggg agccgccagg ccaactgacg gcggttgcgc taagttatac
     7921 cagagcgcat tgccttcatc catgcagccg gtgaatgacg acacgggaag ccacataggc
     7981 acaatatcta agtggaaatg gctgaaggta tggcgaaacg cggtcagttg cgtcaggtta
     8041 tcggcagcaa tctgccgttg tgccagccaa tgccgcaaac tttcttcgtc ggcaaactgc
     8101 gggaaacagt ataaaccgcc ccacaatccg ctcggcggac gctgcgccag caatacttca
     8161 tcttcgtgct gtaacaggag aaagtagccg gtgcgctccg gcagcgtctg tttcggtttt
     8221 ttgcccggat aaagcgacca gctattgttg gtggcggcaa tacatccgtt ttgtagcgga
     8281 cagagcgaac atttcggctt cgaacgcgtg caaatcatcg cgcccaaatc catcatcgcc
     8341 tgattaaacc gttccacgcc gaccgcgggc gtcacctgct cgctcaaact ccatagttta
     8401 ttctcgacct cttttttccc aggccagccg cttacagcat agcagcgcgc cagcacgcgt
     8461 ttgacgttac cgtcgagaat cggaaagtgc ttacccagag aaagcgagag aatcgcgcct
     8521 gcggtggaac gcccgacgcc cggtaacgcc gcgacttctt caaaggtttc cgggaattta
     8581 ccaccgtgta aggtcgccac ttgttgtgcc gctttatgca ggttgcgcgc gcgggcgtaa
     8641 tagccaagcc cggtccacaa gtggagaact tcgtcgagcg gcgcattggc gagatcggtc
     8701 accgtcggga agtgcgccat aaagcgttca aaatagggga taacggtcgc aacctgagtt
     8761 tgttgcaaca tcacttctga gagccatact ttgtagggcg tcttgtcaat ttgccagggc
     8821 agcgttttcc gcccgtattt atcgtaccag tccagaacct gggctgaaaa ttgcgacgct
     8881 tgcatggtca ccgacttcac tgttgttggg ggcaagattg cagcacagcg acggcagggt
     8941 gtaaaccgga actttccgca gccacggcct tatcatgctt gcatccggca actaactttg
     9001 gataatgccc gttttcagaa cactttcaca agcgactaaa tctttatgaa aaacgacgtc
     9061 atttcaccgg aatttgatga aaacggccgc ccactgcgcc gtatccgtag ttttgtgcgc
     9121 cgccaggggc gactgaccaa aggccaggaa catgcgctgg aaaactactg gccggtgatg
     9181 ggcgttgagt tcagtgaaga tatgctggat ttccccgcgc tttttggccg tgaagcgccg
     9241 gtgacgcttg agattggttt tggcatgggg gcgtcgctgg tggcaatggc taaagatcgc
     9301 cccgagcagg acttcctcgg cattgaagtg cattcaccgg gcgttggtgc gtgcctggct
     9361 tctgcgcatg aagaaggttt aagcaacctg cgcgtgatgt gtcacgatgc ggttgaagtg
     9421 ctgcataaaa tgattcctga caattcattg cgcatggtgc agctcttttt ccctgacccg
     9481 tggcacaaag cgcgccataa taaacgccgt atcgttcagg tgccgtttgc cgaactggta
     9541 aaaagcaaac tgcagctggg gggcgtattc catatggcga ccgactggga accttatgcg
     9601 gaacatatgc ttgaagtgat gtcttctatt gacggttata aaaacctgtc agagagcaat
     9661 gattacgtac cgcgtccggc atcacgtccg gtgacgaaat ttgaacaacg tggtcatcgt
     9721 cttggtcacg gagtatggga cttaatgttc gagagggtga aataatggca aagaaccgta
     9781 gccgtcgtct gcgtaaaaaa atgcacatcg acgaattcca ggaattagga ttttcggtgg
     9841 catggcgatt cccggaaggt acatcggaag aacagattga taaaatcgtt gatgacttta
     9901 ttaacgaggt tatcgaaccg aacaaactgg cctttgacgg cagcggttat ctggcctggg
     9961 aaggtctgat ctgcatgcag gaaatcggca aatgcaccga agaacatcag gcgattgtgc
    10021 gtaagtggct ggaagagtgc aaactggatg aggtacgcac cagcgaactt ttcgacgttt
    10081 ggtgggacta agaaagcata cgggcgatga caaatgcaaa actgcctgat gcgctacgct
    10141 tatcaggcct acagagatgc acgatcgagt aggcgggata aggcgtttac gccgcatccg
    10201 gcatggaaaa cgcgtacttt gttatcaatc tggggccagc atatgctggc ctgatttgtt
    10261 cttgagggaa gactatgatg cgcaaaatgc tgctggcggc agcactttca gtgacggcaa
    10321 tgaccgctca cgccgactac cagtgcagcg tcacgccgcg tgacgatgtg attgtcagcc
    10381 cgcaaaccgt gcaggtgaag ggcgaaaacg gcaatctggt gatcacgcca gacggcaacg
    10441 tgatgtataa cggtaagcaa tattccctga atgccgccca gcgcgagcag gcgaaggatt
    10501 atcaggctga actacgtagc acgctgccgt ggattgatga aggcgcgaaa agccgcgtcg
    10561 agaaagcccg tattgcgctg gataaaatta tcgttcagga gatgggcgac agcagcaaaa
    10621 tgcgcagccg tctgaccaaa cttgatgcgc agcttaaaga gcagatgaat cgcattatcg
    10681 aaacgcgcag cgatggcctg acgttccact ataaagccat tgatcaggtt cgcgccgaag
    10741 gccagcaact agtgaatcag gcaatgggcg gaattttaca ggacagcatt aatgaaatgg
    10801 gcgcgaaagc ggtgctgaaa agcggcggta acccattaca gaatgtgctg ggaagcttgg
    10861 gcggtctgca atcctcaatc caaaccgagt ggaaaaagca ggaaaaagat ttccagcagt
    10921 ttggcaaaga tgtttgtagc cgcgttgtga ctctggaaga tagccgcaaa gccctggtcg
    10981 ggaatttaaa ataatcctct attttaagac ggcataatac ttttttatgc cgtttaattc
    11041 ttcgttttgt tacctgcctc taactttgta gatctccaaa atatattcac gttgtaaatt
    11101 gtttaacgtc aaatttccca tgcaaagcta agggataatg cgtagcgtcc acgtaactgg
    11161 aggaatgaaa tggagttttt caaaaagacg gcacttgccg cactggttat gggttttagt
    11221 ggtgcagcat tggcattacc caatatcacc attttagcaa ccggcgggac cattgccggt
    11281 ggtggtgact ccgcaaccaa atctaactac acagcgggta aagttggcgt agaaaatctg
    11341 gttaatgcgg tgccgcaact aaaagacatt gcgaacgtta aaggcgagca ggtagtgaat
    11401 atcggctccc aggacatgaa cgataatgtc tggctgacac tggcgaaaaa aattaacgcc
    11461 gactgcgata aaaccgacgg cttcgtcatt acccacggta ccgacacgat ggaagaaact
    11521 gcttacttcc tcgacctgac ggtgaaatgc gacaaaccgg tggtgatggt cggcgcaatg
    11581 cgcccgtcca cgtccatgag cgcagacggt ccattcaacc tgtataacgc ggtagtgacc
    11641 gcagctgata aagcctccgc taatcgtggc gtgctggtgg tgatgaacga caccgtactg
    11701 gacggtcgcg atgtcaccaa aaccaacacc accgacgtag cgaccttcaa gtctgttaac
    11761 tacggtcctc tgggatacat tcacaacggt aagattgatt accaacgtac cccggcacgt
    11821 aagcacacca gcgatacgcc attcgatgtc tctaagctga atgagctgcc gaaagtcggc
    11881 atcgtttata actacgctaa cgcatccgat cttccggcta aagcactggt agatgcgggc
    11941 tatgatggca tcgttagcgc tggtgtgggt aatggtaacc tgtataaatc cgtgttcgac
    12001 accctggcaa ccgccgcgaa aaacggcact gcagtagtgc gttcttcccg cgtaccgacg
    12061 ggtgctacca ctcaggatgc tgaagtggat gatgcgaaat acggcttcgt cgcctctggc
    12121 acgctgaacc cgcaaaaagc gcgcgtcctg ctgcagctgg ctctgacgca aaccaaagat
    12181 ccgcagcaga tccagcagat tttcaatcag tactaatcac ctcgtcccgg catcgtgccg
    12241 gggctttttc acttcagact tacgtccatt gccaattttc attaccctaa tgataatcac
    12301 cggaataaat tattccgcgc gagggttttc gggtgaaaaa gcaatggatt gttggcacgg
    12361 cgctgcttat gttgatgact ggtaatgcct gggcagatgg cgaaccgcca actgaaaata
    12421 tcttaaaaga tcaattcaaa aagcagtatc acggcattct caagcttgat gccatcagct
    12481 taaaaaatct tgatgctaag ggtaatcagg ccacctggtc agcggaaggc gatgtctctt
    12541 ccagtgacga tctctatacc tgggtcggtc agttggcaga ttacgaactg ctcgaacaga
    12601 cctggacgaa agataaaccg gtaaaattct cggcgatgtt aaccagtaaa ggaacgcccg
    12661 cgtctggctg gtcggtgaac ttttactctt ttcaggcggc agccagcgat cgtgggcggg
    12721 tggtcgacga tatcaaaacg aataataaat atctgatcgt gaatagcgaa gatttcaatt
    12781 atcgctttag tcagcttgaa tctgcgttga atacccagaa gaactcgatt cctgcgctgg
    12841 aaaaggaagt gaaagcgctt gataagcaaa tggtggcggc gcagaaagca gcggatgcat
    12901 attggggaaa agatgcaaac ggtaagcaaa tgacccgcga agaagccttt aaaaaaattc
    12961 accaacagcg tgatgagttt aataaacaga acgatagcga ggcgttcgcg gttaaatatg
    13021 acaaagaggt ttatcaaccg gcgattgcgg catgtcataa acagagtgaa gagtgttatg
    13081 aagtgccgat tcagcagaaa cgagatttcg atatcaacga acaacggcga cagacttttc
    13141 tgcaatcaca aaaactcagc cgtaaattgc aggatgactg ggtaacgctc gaaaaagggc
    13201 aatatccgtt aacgatgaaa gtttcagaaa taaacagtaa aaaagtcgcg attttgatga
    13261 aaattgacga tatcaaccaa gctaatgaac gctagaaaaa agataccgaa caactgcggc
    13321 gcaatggggt gattaagtaa ctcatgttaa gtgcctttgc cggataatat aaaaagttat
    13381 ccggcaaatt attttttaca ttagccctag tagctgtgga atgaacaagg atatttgcgg
    13441 aatgtaagtg atcatcatca acgctactag caatgcagca taaaatagca gcaatggttt
    13501 gattaaatgt tgaatcttca cacccgagat agaacaacca acaaatagcg cactacccac
    13561 cggcggtgtt aacagcccaa tacaaaggtt ggccaccatc ataataccga aatgtactgg
    13621 gtccattccc agctcttgtg caatcggcaa gaagatcggt gtaaagatta gtacagccgg
    13681 tgtcatgtcc ataaagatgc cgacaattaa taagacgata ttaatcagca gcaatatgat
    13741 cagtgggtta tcagaaatcc ccatcaatgc gtcgctgatc atataaggaa tatcagcatt
    13801 agtcattgcc caggacatgc ccacggaaaa accgatcaac agtaatacaa tcgaagtggt
    13861 aacaaccgac tccagaatca gtttgggtaa atggcgccat tttacttccc ggtaaatcag
    13921 caccgagaga ataaatgtgt agacaacggc aattgctgac gcttcagttg cggtaaaaat
    13981 gccgcccaga atgccgccca tcacaatgaa caccagaagc aaacttggta aggcatccag
    14041 aaacgcttta caggcctgag caaaggttgc tctttctgaa agcgggtagc cacggcgttt
    14101 agcaataatg ccgcagacta tcattaccgc aagacccatt aagatccctg gcaaatatcc
    14161 cgccataaac agcgatgcta cagaaacgcc gccagctgtc agggaaaaca caattaacac
    14221 gtttgagggc ggtatcagta atccagtaat acaagatgag acgtttacag cggtagaaaa
    14281 tgccggatca taaccttctt tggtctgaat gggattcagt gtgccaccaa cggcagcagc
    14341 tgccgcaacg gctgaaccag aaatggaacc aaacatcata ttcgccagga cgttcacatg
    14401 accaagcgaa ccaggaacgc ggccaaccat tacctgggcg aggttaatca aacgtatcgc
    14461 aataccgccg ctgttcatca gtgttccggc aaagatgaaa aaggggattg ccaacagggc
    14521 aaagttatcc agtccattag ccagtcgttg cgaaacagtg attgccgcga tatcccaggg
    14581 aaacatcaaa agcattgaag ctacagtggc aataccgatt gagaacgaaa tgggcacacc
    14641 aataaagacc agcacaaaaa aacagccaaa cagtgtaatc gcgatataac tatc
//
LOCUS       DAZPCS010000090        14620 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZPCS010000090 DAZPCS010000000
VERSION     DAZPCS010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14620
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.090"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(87..2063)
                     /gene="speA"
                     /locus_tag="ACHUQ7_002639"
     CDS             complement(87..2063)
                     /gene="speA"
                     /locus_tag="ACHUQ7_002639"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFR1135993.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPVE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2072..2203)
                     /gene="yqgB"
                     /locus_tag="ACHUQ7_002640"
     CDS             complement(2072..2203)
                     /gene="yqgB"
                     /locus_tag="ACHUQ7_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFR1135994.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAATVVNCFTLNKII"
     gene            complement(2297..2440)
                     /gene="yqgG"
                     /locus_tag="ACHUQ7_002641"
     CDS             complement(2297..2440)
                     /gene="yqgG"
                     /locus_tag="ACHUQ7_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFR1135995.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(2551..2802)
                     /gene="yqgD"
                     /locus_tag="ACHUQ7_002642"
     CDS             complement(2551..2802)
                     /gene="yqgD"
                     /locus_tag="ACHUQ7_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFR1135996.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            2858..4012
                     /gene="metK"
                     /locus_tag="ACHUQ7_002643"
     CDS             2858..4012
                     /gene="metK"
                     /locus_tag="ACHUQ7_002643"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFR1135997.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            4449..5843
                     /gene="galP"
                     /locus_tag="ACHUQ7_002644"
     CDS             4449..5843
                     /gene="galP"
                     /locus_tag="ACHUQ7_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFR1135998.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGIGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            5920..6417
                     /locus_tag="ACHUQ7_002645"
     CDS             5920..6417
                     /locus_tag="ACHUQ7_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFR1135999.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            6512..7219
                     /gene="endA"
                     /locus_tag="ACHUQ7_002646"
     CDS             6512..7219
                     /gene="endA"
                     /locus_tag="ACHUQ7_002646"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFR1136000.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            7299..8030
                     /gene="rsmE"
                     /locus_tag="ACHUQ7_002647"
     CDS             7299..8030
                     /gene="rsmE"
                     /locus_tag="ACHUQ7_002647"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFR1136001.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            8043..8993
                     /gene="gshB"
                     /locus_tag="ACHUQ7_002648"
     CDS             8043..8993
                     /gene="gshB"
                     /locus_tag="ACHUQ7_002648"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFR1136002.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTCYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            9102..9665
                     /locus_tag="ACHUQ7_002649"
     CDS             9102..9665
                     /locus_tag="ACHUQ7_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFR1136003.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            9665..10081
                     /gene="ruvX"
                     /locus_tag="ACHUQ7_002650"
     CDS             9665..10081
                     /gene="ruvX"
                     /locus_tag="ACHUQ7_002650"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFR1136004.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(10257..11237)
                     /locus_tag="ACHUQ7_002651"
     CDS             complement(10257..11237)
                     /locus_tag="ACHUQ7_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFR1136005.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQKVGMLTFQQSYQQRVGEGRL"
     gene            11255..11959
                     /gene="yggS"
                     /locus_tag="ACHUQ7_002652"
     CDS             11255..11959
                     /gene="yggS"
                     /locus_tag="ACHUQ7_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFR1136006.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNEQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPALESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            11977..12543
                     /gene="yggT"
                     /locus_tag="ACHUQ7_002653"
     CDS             11977..12543
                     /gene="yggT"
                     /locus_tag="ACHUQ7_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFR1136007.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQCAHCDFYTPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYAINMGVAEVLQATGNMLLPGLWMAL"
     gene            12540..12830
                     /gene="yggU"
                     /locus_tag="ACHUQ7_002654"
     CDS             12540..12830
                     /gene="yggU"
                     /locus_tag="ACHUQ7_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFR1136008.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            12838..13431
                     /locus_tag="ACHUQ7_002655"
     CDS             12838..13431
                     /locus_tag="ACHUQ7_002655"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFR1136009.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            13424..14560
                     /gene="hemW"
                     /locus_tag="ACHUQ7_002656"
     CDS             13424..14560
                     /gene="hemW"
                     /locus_tag="ACHUQ7_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFR1136010.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
BASE COUNT         3523 a         3870 c         3754 g         3473 t
ORIGIN      
        1 agcagctaga ttatttttaa gaatatataa aaagaaagcg ggccgattta cgtaaataaa
       61 tcgacccgcc agcggaataa tcttaattat tcatcttcaa gataagtata accgtacaaa
      121 cctgcctcga actcttcaag gaactgttgt tgcagttcag catcaagatc ggttttcttc
      181 acttgatcgc ggaactgggt taacagcgtt ttcggatcga gctgtacata ttgcagcatg
      241 tccgccacgg tatcgccttc gtcagacagt tctacttcta cgctaccgtc agggaacacg
      301 aacacgtcaa ctgcttcggt atcaccgaac aggttgtgca tattgccaag gatctcctga
      361 tatgcaccga ccataaagaa accgagcatc ggcggattct ctggatcgta ctccggcatt
      421 ggcattgtcg tggcaatacc atcaccatca atatagtggt cgatagcacc gtcagagtca
      481 caggtaatat ccagcagcac agcgcgacgt tccggcactt gatccagccc ttccagcggt
      541 agaaccggga acaactggtc gatcccccat gcgtccggca ttgactggaa cagcgagaaa
      601 ttgacgtaca ttttgtccgc catacgttcc tgcagttcgt cgattatcgg acgatgcgca
      661 cggttttgcg gatccagttg cttctgcact tcatggcaca tgctcagata gagttgttct
      721 gcccatgcac gttcttgcag gctaaaggtg ccggaagagt agccgatatg aatgtcgtgc
      781 agatccatct ggctgtcgtg taaccattca cgcaaagaac ggcgagttcc cggttcgtgc
      841 atttcctgcc aggtttccca catgctttgc agcgcacgcg gcgcatcttc tacgggcgcg
      901 gtcggcaccg tatattcgtt acgttccacg ccgatgatat tcgacaccag cacggtatgg
      961 tgcgcagtca ccgcacggcc cgattcggtg attaccgtcg gatgcggcag accattttct
     1021 tcacacgcat cgccaatcgc ccagatgatg ttgttggcgt attcattgag gccatagttt
     1081 acggagcagt cagactgcga acgggtgcct tcataatcca cgcccagacc gccgccgacg
     1141 tcgaagcact gaatattgac gcccagcttg tgcagttcca catagaaacg cgcggattca
     1201 cgaacgcctg tcgcgatatc gcgaatattc gccatctgcg aaccgaggtg gaagtgcagt
     1261 agttgcaggc tgtcgagacg cccggcttca cgcagggttt caaccagttg cagtacctga
     1321 gtcgcagcca ggccgaactt cgatttttcc ccgccggagg actgccattt acccgagccc
     1381 tgcgaagcca gacgtgcacg cacgcccaga cgaggaacga cattcagacg ttctgcttca
     1441 tccagcacaa tggcgatttc tgacatcttc tcaatgacca gatagacctt gtgccccatc
     1501 ttctcgccaa ttaatgccag gcggatatat tcgcggtctt tataaccgtt gcagacgatg
     1561 acgctacgag tcatgccagc atgtgccagc actgccatca actcggcttt ggaaccggct
     1621 tccagaccca gcggttcacc cgaatgaatc agggactcga tcacgcggcg gtgctggtta
     1681 actttgatcg gataaacaag gaagtaatcg ccgttatagc cgtaggattc cctcgcacgt
     1741 ttgaacgcgg cgttaatgga acgcaaacgg tgctgcagga tctgtgggaa acagaacagt
     1801 gcaggcagac gctgaccctg tgcttcacga gttttcacta actgcgcgag atcgacgcga
     1861 gcttccggga cgtccgggtc cgggcacacg ctgatgtggc ccagctcgtt aacgtcatag
     1921 tagttattgc cccaccaggc aatattgtaa gtacgcagca tcttgctggc ttcctgggag
     1981 ctcattgcaa cctcctgcat ggagcgtagt acaccgtgtt cgcccgctga cgaaggcaaa
     2041 cccatagaca tgtcgtcaga catagcgaac ctcaaattat tttattaagt gtaaaacagt
     2101 taacgactgt cgcagcctgg aactgcgata acaacccata agcacatgga ttttccagca
     2161 gtgtatgctg acgctccgac tgcgcaaccg gtttcttttt cataacatta ttaagcacat
     2221 aaccgaacgt aagtgtgaca gttcggcgaa accacgagaa aactcttgtt tttacaagag
     2281 cgcccttgtt cagtcctcag taactgtaac cagctcttga atcctgagaa gcgccgagat
     2341 gggtataaca tcggcaggta tgcaaagcag agatgcagag tgcggggaac gagtcttcac
     2401 cagaacggtg agacaggtta agcagcagac aacggttcat tatttcgtat cacctccacg
     2461 gccgcctgtt aagacgaacc cacaagccaa aactctgatt tcaacccggc tggaagtggc
     2521 aacacgaaag aaacgtcgtg tgctttttat ttaagccgcg cgccgcgttt tataccccac
     2581 aatggcagaa aattgcaaaa gataaatacg cagaatgccg gcattgtcag gaaaaatttc
     2641 cagccacgtt tttaacagaa tgagacacga ttcaaaaaaa agtggaaatc gggtgaagaa
     2701 ttgacctaaa atagccatcc agatgttaat ccatccatac cgattaacac tcagactgcc
     2761 agtgttttta acctgcagag tcgtggtagg atccgctacc acagaaaatc cacacaacag
     2821 tttgagctaa ccaaattctc tttaggtgat attaaatatg gcaaaacacc tttttacgtc
     2881 cgagtccgtc tctgaagggc atcctgacaa aattgctgac caaatctctg atgccgtttt
     2941 agacgcgatc ctcgaacagg atccgaaagc acgcgttgct tgcgaaacct acgtaaaaac
     3001 cggcatggtt ttagttggcg gcgaaatcac caccagcgcc tgggtagaca tcgaagagat
     3061 cacccgtaac accgttcgcg aaattggcta tgtgcattcc gacatgggct ttgacgctaa
     3121 ctcctgtgcg gttctgagcg ctatcggcaa acagtctcct gacatcaacc aaggcgttga
     3181 ccgtgccgat ccgctggaac agggcgcggg tgaccagggt ctgatgtttg gctacgcaac
     3241 taatgaaacc gacgtgctga tgccagcacc tatcacctat gcacaccgtc tggtacagcg
     3301 tcaggctgaa gtgcgtaaaa acggcactct gccgtggctg cgcccggacg cgaaaagcca
     3361 ggtgactttc cagtatgacg acggcaaaat cgttggtatt gatgctgtcg tgctttccac
     3421 tcagcactct gaagagatcg accagaaatc gctgcaagaa gcggtaatgg aagagatcat
     3481 caagccaatt ctgcccgctg aatggctgac ttctgccacc aaattcttca tcaacccgac
     3541 cggtcgtttc gttatcggtg gcccaatggg tgactgcggt ctgactggcc gtaaaattat
     3601 cgttgatacc tacggcggca tggcgcgtca cggtggcggt gcattctctg gtaaagatcc
     3661 atcaaaagtg gaccgttccg cagcctacgc agcacgttat gtcgcgaaaa acatcgttgc
     3721 tgctggcctg gccgatcgtt gtgaaattca ggtttcctac gcaatcggcg tggctgaacc
     3781 gacctccatc atggtagaaa ctttcggtac tgagaaagtg ccttctgaac aactgactct
     3841 gctggtacgt gagttcttcg acctgcgccc atacggactg attcagatgc tggatctgct
     3901 gcacccgatc tacaaagaaa ccgcagcata cggtcacttt ggtcgtgaac atttcccgtg
     3961 ggaaaaaacc gacaaagcgc agctgctgcg cgatgctgcc ggtctgaagt aatctttctt
     4021 cacctgcgtt caaaggccag cctcgcgctg gcctttttct tttacctcac cgcggtggat
     4081 aggcgttcac gccgcatccg gcaaaaaaac tgcccgcaca ataacatcat tcttcctgat
     4141 cacgtttcac ggcaaattac catcacaact gaaaccgatt acaccaacca caccagcaaa
     4201 atatttgtaa tattttcata ttattattca gctttcacag ttgttacatt tcttttcagt
     4261 aaagtcttaa ttgcagatga cagcgtttaa tctatgatga tataactcaa ttattttcat
     4321 gcccttaaat cataactaag ataaatgtta gtgtaagcga ttacactgat gtgatttgct
     4381 tcacatcttt ttacgtcgta ctcacctatc ttaattcaca ataaaaaata actatattgg
     4441 agggcatcat gcctgacgct aaaaaacagg ggcggtcaaa caaggcaatg acgtttttcg
     4501 tctgcttcct tgccgctctg gcgggattac tctttggcct ggatatcggt gtaattgctg
     4561 gcgcactgcc gtttattgca gatgaattcc agataacttc gcacacgcaa gaatgggtcg
     4621 taagctccat gatgttcggt gcggcagtcg gtgcggtggg catcggctgg ctctccttta
     4681 aactcgggcg caaaaagagc ctgatgatcg gcgcaatcct gtttgttgcc ggttcactgt
     4741 tctctgcggc tgcgccaaac gttgaagtac tgattctttc gcgcgttctg ctggggctgg
     4801 cggtgggtgt ggcctcttat accgcaccac tgtacctgtc agaaattgcg ccggaaaaaa
     4861 ttcgcggcag tatgatctcg atgtatcagt tgatgatcac tatcgggatc ctcggtgctt
     4921 atctttctga taccgccttc agctacaccg gtgcatggcg ctggatgctg ggtgtgatta
     4981 tcatcccggc aattttgctg ctgattggtg tcttcttcct gccagacagc ccacgttggt
     5041 ttgccgccaa acgccgtttt gttgatgccg aacgcgtgct gctacgcctg cgtgacacca
     5101 gcgcggaagc gaaacgcgaa ctggatgaaa tccgtgaaag tttgcaggtt aaacagagtg
     5161 gctgggcgct gtttaaagag aatagcaact tccgccgcgc ggtgttcctt ggcgtactgt
     5221 tacaggtaat gcagcaattc accgggatga acgtcatcat gtattacgcg ccgaaaatct
     5281 tcgaactggc gggttatacc aacaccaccg agcaaatgtg ggggacagtg attgtcggcc
     5341 tgaccaacgt acttgccacc tttatcgcaa tcggccttgt tgaccgctgg ggacgtaaac
     5401 caacgctaac gctgggcttc ctggtgatgg ctgctggcat gggcgtactc ggtacaatga
     5461 tgcatatcgg tatccactct ccgtcggcgc agtatttcgc catcgccatg ctgctgatgt
     5521 ttattgtcgg ttttgccatg agtgccggtc cgctgatttg ggtactgtgc tccgaaattc
     5581 agccgctgaa aggccgcgat tttggcatca cctgctccac cgccaccaac tggattgcca
     5641 acatgatcgt tggcgcaacg ttcctgacca tgctcaacac gctgggtaac gccaacacct
     5701 tctgggtgta tgcggctctg aacgtactgt ttatcctgct gacattgtgg ctggtaccgg
     5761 aaaccaaaca cgtttcgctg gaacatattg aacgtaatct gatgaaaggt cgtaaactgc
     5821 gcgaaatcgg cgctcacgat taatctcctc aggcttcctc ccgtcgcgga ggaagccgcc
     5881 tcttgcagtc atcttttctt cgctctatcc tctgccgcta tgaaaacatc ccgtctccct
     5941 atcgccatcc aacaggccgt tatgcgccgc ctgcgggaaa aactcgccca ggccaacctg
     6001 aagctagggc gtaactaccc ggagccaaaa ctctcttaca cccagcgcgg aacctccgcc
     6061 ggaacagcct ggctggaaag ctatgaaatc cgcctcaatc ccgttttgct gttggaaaac
     6121 agtgaagctt ttattgaaga agtggtaccg cacgaactgg cacatttgct ggtatggaaa
     6181 catttcggcc gcgtagcgcc acatggcaaa gagtggaagt ggatgatgga aagcgtgctg
     6241 ggcgttcccg cccgtcgtac gcatcagttc gaactgcaat ccgtgcgtcg caacaccttc
     6301 ccctaccgct gtaagtgcca ggaacatcag cttaccgtac gccgccataa tcgcgtagtt
     6361 cgtggcgagg ccatctatcg ctgtgttcac tgcggtgaac agctggttgc gaaataacca
     6421 cctgaactat caggaacttt cctgatctgg ctgattgcat accaaaacaa ctttcgctac
     6481 gttgctggct cgttttaaca cggagtaagt gatgtaccgt tatttgtcta ttgctgcggt
     6541 ggtactgagc gcagcatttt ccggcccgac gttggccgaa ggtatcaata gtttttctca
     6601 ggcgaaagcc gcggcggtaa aagtccacgc tgatgcgccc ggtacgtttt attgcggatg
     6661 taaaattaac tggcagggca aaaaaggcgt tgttgatctg caatcgtgcg gctatcaggt
     6721 gcgcaaaaat gaaaaccgcg ccagccgcgt agagtgggaa cacgtcgttc ccgcctggca
     6781 attcggtcac cagcgccagt gctggcagga cggtggacgt aaaaactgcg ctaaagatcc
     6841 ggtctatcgc aagatggaaa gcgatatgca taacctgcag ccgtcagtcg gcgaggtgaa
     6901 tggcgatcgc ggcaatttta tgtacagcca gtggaatggc ggcgaaggcc agtacggtca
     6961 atgcgccatg aaggtcgatt tcaaagaaaa agctgccgaa ccaccagcgc gtgcacgcgg
     7021 tgccattgcg cgcacctact tctatatgcg cgaccaatac aacctgacac tctctcgcca
     7081 gcaaacgcag ctgttcaacg catggaacaa gatgtatccg gttaccgact gggagtgcga
     7141 gcgcgatgaa cgcatcgcga aggtgcaggg caatcataac ccgtatgtgc aacgcgcttg
     7201 ccaggcgcga aagagctaac ctacactagc gggattcttt ttgttaaccc ctaccccacg
     7261 cgtacaaccg cgtggggaga cgacgcggat ttttaactat gcgtatcccc cgcatttatc
     7321 atcctgaacc actgaccagc cattctcaca tcgcgctttg cgaagatgcc gccaaccata
     7381 tcgggcgcgt actgcgcatg gggccggggc aggcgttgca attgtttgac ggtagcaacc
     7441 aggtctttga cgccgaaatt accagcgcca gcaaaaaaag cgtggaagtg aaggtgctgg
     7501 aaggccagat cgacgatcgc gaatctccgc tgcatattca cctcggtcag gtgatgtcgc
     7561 gtggtgaaaa aatggaattt actatccaga aatcgatcga actcggtgta agcctcatta
     7621 cgccactttt ttctgagcgc tgcggcgtta aactggatag tgaacgtctg aacaagaagc
     7681 ttcagcagtg gcagaagatt gcaattgctg cctgtgagca gtgtggtcgt aaccgggtgc
     7741 cggaaatccg tccagcgatg gatctggaag cctggtgtgc agagcaggat gaaggactga
     7801 aactgaatct tcacccgcgc gccagtaaca gcatcaatac gttgccttta ccggttgaac
     7861 gcgtccgcct gctgattggc ccggaaggcg gtttatcggc agatgaaatt gccatgactg
     7921 cccgctatca atttactgat atcctgttgg gacctcgcgt tttgcgtaca gagacaactg
     7981 cgctcaccgc cattaccgcg ctacaagtac gatttggcga tttgggctaa cggagaagaa
     8041 taatgatcaa gctcggcatc gtgatggacc ccatcgcaaa catcaacatc aagaaagatt
     8101 ccagtttcgc tatgttgctg gaagcacagc gtcgtggtta cgaacttcac tatatggaga
     8161 tgggcgatct gtatctgatc aatggcgaag cccgcgccca tacccgcacg ctgaacgtga
     8221 agcagaacta cgaagagtgg ttttcgttcg tcggtgaaca ggatctgccg ctggccgatc
     8281 tcgatgtgat cctgatgcgt aaagacccgc cgtttgatac cgagtttatc tacgcgacct
     8341 atattctgga acgtgccgaa gagaaaggga cgctgatcgt taacaagccg cagagcctgc
     8401 gcgactgtaa cgagaaactg tttaccgcct ggttctctga cttaacgcca gaaacgctgg
     8461 ttacgcgcaa taaagcgcag ctaaaagcgt tctgggagaa acacagcgac atcattctta
     8521 agccgctgga cggtatgggc ggcgcgtcga ttttccgcgt gaaagaaggc gatccaaacc
     8581 tcggcgtgat tgccgaaacc ctgactgagc atggcacttg ctactgcatg gcgcaaaatt
     8641 acctgccagc cattaaagat ggcgacaaac gcgtgctggt ggtggatggc gagccggtac
     8701 cgtactgcct ggcgcgtatt ccgcaggggg gcgaaacccg tggcaatctg gctgccggtg
     8761 gtcgcggtga acctcgtccg ctgacggaaa gtgactggaa aatcgcccgt cagatcgggc
     8821 cgacgctgaa agaaaaaggg ctgatttttg ttggtctgga tatcatcggc gaccgtctga
     8881 ctgaaattaa cgtcaccagc ccaacctgta ttcgtgagat tgaagcagag tttccggtgt
     8941 cgatcaccgg aatgttaatg gatgccatcg aagcacgttt acagcagcag taacccacct
     9001 tagcgagaag gatctcgttg agactctgag tgacagcgcc cttctttcca cgcatactgg
     9061 gcgctgttgc ttttttgaac caggaaacag aacctctgac aatgaattta cagcatcact
     9121 ttcttattgc catgcctgct ctccaggatc cgattttccg tcgttccgtg gtctacattt
     9181 gcgaacataa taccaatggt gcaatgggga tcatcgtcaa caagccgctg gaaaatctca
     9241 aaattgaagg gattctggaa aagctgaaga tcacgccgga gccgcgtgat gaatcaatcc
     9301 gtctggataa accggttatg ctcggcggcc cactggctga agatcgcggg tttattttgc
     9361 atactccgcc ctccaatttt gcttccagca ttcgcatttc agacaacacg gtgatgacca
     9421 cttcccgcga tgtgctggaa acgctcggca ccgataaaca accgtctgac gtattggtgg
     9481 ctctgggcta tgcctcctgg gaaaaaggtc aactggaaca agaaattctc gataacgcgt
     9541 ggctaacggc cccggcggat ctgaatattc tgttcaaaac gccgattgcc gaccgctggc
     9601 gcgaggcggc aaaactgatt ggtgtggata ttctcaccat gcctggtgtg gcaggacacg
     9661 cctgatgagt ggaaccttac tcgccttcga cttcggcacc aaaagcattg gcgtagcggt
     9721 cggccaacgc attaccggca ccgctcgccc tttgcctgca attaaagcgc aggacggtac
     9781 gccggactgg aacctcatcg aacgtttact gaaagagtgg cagcctgacg aaatcatcgt
     9841 cggtttgccg ctgaatatgg acggcaccga acagccgtta accgccagag cgcgcaaatt
     9901 tgccaaccgt attcatggtc gtttcggtgt tgaagtaaag ctccatgacg agcgtcttag
     9961 cactgtggaa gcccgttccg gtctttttga acagggcggc tatcgggcgc tcaacaaagg
    10021 caaagttgac tctgcctctg cggttattat tctcgaaagc tatttcgagc agggatatta
    10081 aggcgatcta aaagcctggc ggatagtcgt taccgccatc ttgccggatg cggcgtgaac
    10141 gccttatcca gcacacatct ggcagcggct gtaggtctga taagacgcgt tagcgtcgca
    10201 tcagacattt attgccttta taggccctat aaacttgcgc atcgggcatg gcaacgtcac
    10261 aaacgccctt cccccacccg ctgctgataa ctctgctgaa acgttaacat ccccaccttc
    10321 tgcccggttt gaataacatg cggtaactgg tgggttttcc cttcgcgaat caaattcccc
    10381 accgcgggag tgttaatcag taattcaaac agcgccacgc gtccttcctg tttatccact
    10441 tccagctttt gtgacagcac tgcccgtaaa ctacccgcaa gttgattacg tacgggatct
    10501 ttttcctgcg ccggaaatga atccaccagt cgctcaactg cctgcgcggc accacgtgta
    10561 tgtaaggttg ccagcaccag atgcccggtt tctgccgccg tcagcgccag acggattgtc
    10621 tcactgtcac gtagctctcc gagcaaaatc acatcaggat cttcccgcaa tgcagcccgc
    10681 aaccccgatg cgaacgtcat acagtgcaaa ccaatttccc gctgctggat caaacatcgc
    10741 tggctggcat agagatattc cacaggatct tccagcgtca gaatatgcgc atcggcatgt
    10801 tgattaagat agccaaccat cgccgccagc gtggtagatt tgccactccc cgtagccccc
    10861 gtcaccagaa tcaggccatt ctcgctcttg agtaattccg gcaataccgg tggtgcacca
    10921 agctgttcga gctgtggaca gtgcgaaggt aacaaccgta atgccagaga aataccttgc
    10981 cgttgcgcga acgcactgcc acgcaaccgc tggttttccg ccagcgacac ggcaaaatcc
    11041 agctggccat tttccagcaa tattgcccgc tgatcgtcat ccagccactc ccgcagtagc
    11101 tcttcgacgt ccggcgcatc aaacggcgca gcttccattc tgccacgaat gcgccatcgt
    11161 gcgggccagg cgctgcacag gtgtagatcc gagacgttat gctttacact aagggccaca
    11221 atttcttcca tattcatact aagatcctcg gaaaatgaac gatattgcgc ataacctggc
    11281 acaggtccgg gacaaaatct cagcggctgc aacgcgttgc ggccgttctc cagaagaaat
    11341 tacgctgctt gcagtcagta aaacaaaacc tgcgagcgcc atcgcagaag ccattgatgc
    11401 cgggcagcgt caatttggtg aaaactacgt tcaggaaggg gtagataaaa ttcgccactt
    11461 tcaggaactg ggcgtaacag gattagaatg gcattttatt ggcccgttgc agtctaataa
    11521 aagccgccta gtggcagagc atttcgactg gtgtcatacc atcgaccgtt tgcgcattgc
    11581 tacccgcctc aacgagcagc gcccggcaga acttccccct cttaacgtac tgattcaaat
    11641 taatattagt gatgaaaaca gtaagtccgg gattcaactg gcagaactgg acgagctggc
    11701 agcggcggtc gctgaactac cgcgtttacg tctgcgcggg ctgatggcaa tccctgcgct
    11761 tgagtcagaa tatgtaaggc agtttgaagt tgcacgccaa atggctgtag catttgccgg
    11821 actgaaaacg cgctacccgc atatcgacac gctctctctg ggaatgtcgg acgatatgga
    11881 agccgccatt gcggcaggta gcacgatggt tcgtatcggc actgcaattt ttggtgcgcg
    11941 tgattactct aaaaaataag gaattaaagg aacgccatga atacgttgac tttcctgctt
    12001 tcaacggtca ttgagctgta tacaatggtg ctgttattac gcatctggat gcagtgtgct
    12061 cattgtgatt tttacacccc cttctcacag tttgtagtga aggtagcgca gccaattatc
    12121 gggccactgc gccgcgttat tccggcaatg ggtcccattg acagcgcctc gctgctggtt
    12181 gcctatattc tcagttttat caaagccatt gtgctgtttg aagtggtgac cttcctgcca
    12241 atcatctgga ttgccggttt actgattctg ctgaaaacca tcggcttgct gattttctgg
    12301 gtcctgctgg tgatggcgat tatgagctgg gtaagccagg gtcgtagccc gattgaatac
    12361 gtgctgattc agctggccga tccgctgctg cgcccgattc gccgcttgct accggcaatg
    12421 ggtgggattg atttctcgcc gatgatcctc gttctgctgc tgtatgccat caatatgggt
    12481 gtcgcagaag tattacaggc aaccggaaac atgctgctgc cggggctgtg gatggcgtta
    12541 tgagtgccgt aacagttaat gatgacggtc tggttttacg gctctatatt cagccgaaag
    12601 ccagccgtga ttctattgtc ggtttacatg gcgacgaagt taaagtcgcc attaccgcgc
    12661 cgccggttga cggccaggcc aacagtcatc tggtgaagtt tctcggtaag caattccggg
    12721 ttgccaaaag ccaggtggtg attgaaaaag gcgaacttgg ccgccacaaa caaattaaaa
    12781 tcattaatcc gcaacaaatc ccgccagaaa tcgcggcgtt aattaattag gtatcctatg
    12841 caaaaagttg tcctcgcaac cggcaatgcc ggtaaagtgc gtgagctggc gtcgctgctt
    12901 agcgactttg gtcttgatat cgtggcccaa acagacctcg gcgttgattc cgctgaagaa
    12961 accggcctaa cctttatcga aaacgcgatt ctgaaagcgc gccatgcggc aaaagtgacc
    13021 ggtttaccgg caattgccga cgactctggt ctggcggtag atgcgctggg tggcgcgccg
    13081 ggtatttact ccgcgcgtta ttccggtgaa gacgcgaccg atcaaaagaa tctgcaaaaa
    13141 ctgttggaaa cactgaaaga cgtaccggac gaccaacgtc aggcgcgttt ccactgcgtg
    13201 ctggtatatc tgcgtcacgc ggaagatccg actccgctgg tatgccacgg cagctggccg
    13261 ggcgtgatta ctcgcgaacc agcgggcact ggtggctttg gttatgatcc aatcttcttc
    13321 gtaccttccg aagggaaaac cgccgccgaa ctgacccgcg aagaaaagag cgccatttcc
    13381 caccgtggtc aggcgttgaa actgctgctg gacgctttac gtaatggtta aattaccgcc
    13441 gctgagtctc tacattcaca tcccgtggtg cgtgcagaaa tgcccgtact gcgatttcaa
    13501 ctctcacgcg ttgaaaggag aagtgccgca cgacgattat gttcagcatc tgctttgcga
    13561 tctggacaat gatgtggctt acgctcaggg ccgtgaagta aagacaatct ttattggcgg
    13621 tggtacgccg agcctgcttt ccggcccggc gatgcaaacg ctgctagacg gcgtgcgtgc
    13681 gcgtttgccg ctggcagcgg atgcagaaat tactatggaa gcgaacccag gcacggtaga
    13741 agcggatcgc tttgtcgatt atcagcgtgc tggtgtaaac cgcatctcta ttggtgtgca
    13801 gagttttagc gaagaaaagc tgaaacgact tgggcgtatt catggcccgc aagaagcgaa
    13861 acgagcggca aagctggcga gcggtttagg gttacgtagc tttaaccttg atttgatgca
    13921 tggactaccg gatcaatcac tggaagaggc gcttggcgat ctacgccagg ccattgaact
    13981 gaatccgccg catctttcct ggtatcaact gactatcgaa cctaatacgc tgtttggctc
    14041 gcgaccgccg gtgctgccgg acgatgacgc gctgtgggat attttcgaac aggggcatca
    14101 gttattaacc gcagcgggtt atcagcaata tgaaacttcc gcttacgcca aacccggtta
    14161 tcagtgccag cacaatctca actactggcg ctttggtgac tacatcggta ttggctgcgg
    14221 cgcgcacggc aaagtgacct tcccggatgg gcgcattctg cgtaccacta aaacgcgtca
    14281 tcctcgtggt tttatgcaag gaaggtatct ggaaagccag cgtgatgtcg aagccgcaga
    14341 taagccgttt gagttcttta tgaatcgctt ccgtctgctg gaggccgcgc cgcgcgtgga
    14401 gtttattgcg tataccgggc tttgcgaaga tgtgattcgc ccacagttag acgaggcgat
    14461 tgcccagggt tatctcaccg aatgtgcgga ttactggcag ataacggaac atggtaaatt
    14521 attcttaaac tcacttcttg aattattcct cgctgagtaa tcattaatgc gcctgcgggc
    14581 gcattaatca cttcttttat ccccccgtta attaacctcg
//
LOCUS       DAZPCS010000091        14464 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZPCS010000091 DAZPCS010000000
VERSION     DAZPCS010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14464
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.091"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..185
                     /locus_tag="ACHUQ7_002657"
     CDS             <1..185
                     /locus_tag="ACHUQ7_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HFR1136011.1"
                     /translation="EWQKDVDLHQVLWSRSRLGERPKGQGITGADHFWFGHTPLRHRV
                     DIGNLHYIDTGAVFGG"
     gene            complement(210..401)
                     /locus_tag="ACHUQ7_002658"
     CDS             complement(210..401)
                     /locus_tag="ACHUQ7_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFR1136012.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGSCF
                     PIEDFIDGFWQPAQEYGDF"
     gene            complement(506..742)
                     /locus_tag="ACHUQ7_002659"
     CDS             complement(506..742)
                     /locus_tag="ACHUQ7_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HFR1136013.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(860..2299)
                     /gene="rsmF"
                     /locus_tag="ACHUQ7_002660"
                     /pseudo
     CDS             complement(860..2299)
                     /gene="rsmF"
                     /locus_tag="ACHUQ7_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
     gene            complement(2379..4999)
                     /gene="yebT"
                     /locus_tag="ACHUQ7_002661"
                     /pseudo
     CDS             complement(2379..4999)
                     /gene="yebT"
                     /locus_tag="ACHUQ7_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
     gene            complement(4968..6251)
                     /gene="yebS"
                     /locus_tag="ACHUQ7_002662"
     CDS             complement(4968..6251)
                     /gene="yebS"
                     /locus_tag="ACHUQ7_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFR1136014.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGLCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSILDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            6381..6878
                     /gene="msrC"
                     /locus_tag="ACHUQ7_002663"
     CDS             6381..6878
                     /gene="msrC"
                     /locus_tag="ACHUQ7_002663"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFR1136015.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6975..7673
                     /gene="proQ"
                     /locus_tag="ACHUQ7_002664"
     CDS             6975..7673
                     /gene="proQ"
                     /locus_tag="ACHUQ7_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFR1136016.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7693..9741
                     /gene="prc"
                     /locus_tag="ACHUQ7_002665"
     CDS             7693..9741
                     /gene="prc"
                     /locus_tag="ACHUQ7_002665"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFR1136017.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLSKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9933..10814
                     /gene="htpX"
                     /locus_tag="ACHUQ7_002666"
     CDS             9933..10814
                     /gene="htpX"
                     /locus_tag="ACHUQ7_002666"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFR1136018.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMMMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10860..12233)
                     /locus_tag="ACHUQ7_002667"
     CDS             complement(10860..12233)
                     /locus_tag="ACHUQ7_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1136019.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMATGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            12410..13201
                     /gene="kdgR"
                     /locus_tag="ACHUQ7_002668"
     CDS             12410..13201
                     /gene="kdgR"
                     /locus_tag="ACHUQ7_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFR1136020.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLQCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(13344..13583)
                     /locus_tag="ACHUQ7_002669"
     CDS             complement(13344..13583)
                     /locus_tag="ACHUQ7_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFR1136021.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLEITDCPLLRKSNIVVDNG"
     gene            13742..13885
                     /gene="mgrB"
                     /locus_tag="ACHUQ7_002670"
     CDS             13742..13885
                     /gene="mgrB"
                     /locus_tag="ACHUQ7_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFR1136022.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            13960..14247
                     /locus_tag="ACHUQ7_002671"
     CDS             13960..14247
                     /locus_tag="ACHUQ7_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFR1136023.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
BASE COUNT         3800 a         3523 c         3705 g         3436 t
ORIGIN      
        1 atgaatggca aaaggacgtt gatttgcatc aggtcttgtg gagccgctcg cgattaggtg
       61 aacgcccaaa agggcaggga attacaggtg ctgatcattt ctggtttggt catacaccgt
      121 tgcgacatcg cgtggatatt ggcaacctgc attatattga taccggtgct gtctttgggg
      181 gctaactgac tcttgtgcaa ctgcaataat taaaaatcac catactcctg tgctggttgc
      241 cagaaaccat ctataaaatc ctcaatcgga aaacaactgc catggcggat ccgttgatcg
      301 ctcatagaat aaagacactg ctgttccgtg ttgtagacat ccacaacaat atcttcacaa
      361 ccgccatcca ggtagcaaac aaaaagtacc agcgcgaaca tttcatcccc gaagtgtggt
      421 gccgtaccgt taagtttagg agagatttta caacggggga ataaccagga caaataaccc
      481 gccatgccga cgggttcttt ttggatcagg caagacgcat aatccaggca tcagattgtt
      541 gatcgtaacg ggtacggtaa aggacagagt gttcgccatt tagcagggca gggatactgg
      601 tttcagcatc ccaggcatcc agttgcatac ataaatcggg gtcagattta caaggaatgg
      661 ttaacgttcg atcacccggc gattcaccgt gtaattcgcc gtcgtcgatt tcaaaagcgc
      721 ctatgcgcac actggttttc atcttcgtgc tctccattta gctgctgatt gtagtacgac
      781 cagtccgatc gcacaatttt tcagcctagt tgaggccggc gaatgtgcca gtcaaaaagt
      841 gcgctttttt tgtgcgctgt catgcgttac tggtaaaaag tttcccgtcc cgcaccagtt
      901 cacgcgggta gctgtttttc agtcgcgaac caatccgttt ggctaaacca atcggctgat
      961 gctggaaagt aaccaacacg tcatccgcca ctggcgcggc ttgcgggtaa acatcgcgcc
     1021 cgcgatacca ctcctacgct tcctgcggtg tcagttcaaa agcgtttaca ttgtcggggg
     1081 aggcatgggc aataaccgct tcatgctgcc agcgataacc tttgttatgc gtttcggcaa
     1141 gtttaatccc taagcgagaa aatcggactt taccgatcag ggcttcaatg cctaccggga
     1201 acaaccacag ttctttgtcg cgttgccaga gtcgcaggtt tccatcccag tttaagccaa
     1261 cacttgcagc cgcctgacga atttgtccgg cttcgcgatc tttcaccggg ctgaacggga
     1321 aattgcccac tttgtatttt ggggcgggta aggcggggat cgcctgagtt ttacgcagac
     1381 gagcaacgaa gaagccttcg cagtcgtaaa tttgtgggaa aacatgcaaa aaaccttctt
     1441 cggtcagcgc cttgtttgca ccagggaaga gatcgccaag tggtaaaaac tctacttcgt
     1501 cggggtaagt ctctttcagc caccggcaaa cggcttcgtt ttcttcccgg tttaaggtat
     1561 aggtcgagta aaccagcgta ccgccaggac gtaatgcatg aaaggcgctg tcaatcagtt
     1621 cccgttgggt agctgcgatt tcctgattgc tttcgggtga ccagtttttt agcgcatcgg
     1681 gatctttacg caccacgcct tcgccggagc agggagcgtc cagcaaaatg gcatcaaaca
     1741 tttctggcac tgccgcacca aacacgcggc caccaaaatg tgtgagcgca acattactga
     1801 tgccacagcg gctgatattg gcatgtaaca cttttaccca actggcggaa aactcattgg
     1861 caaggattgc cccttcgtta ttcatccgcg cggcaatttg cgtcgttttt gagcctggcg
     1921 cagcagcgac atccatcacc cgctgtggtg cattaccgtc agcaaacaag gcggcgacgg
     1981 gcaacattga actggcttcc tgaatataaa acaggccact taaatgctcg gtggtactac
     2041 ccaatggcaa tgcatcttca ttgtcgcgtt caatccagaa accttcttca caccacggaa
     2101 ttggcgtaag cgtccagcca taaggagcgg ttaattgcag gaaatcagca acggagattt
     2161 tcagcgtatt aacgcgaatg ctgcggcgca acgggcgctg acaggcggca agaaaatcat
     2221 caaatgagag cgtcgaaggc atagcttcgc gcatttgtgt cagaaacgcg tccgggaaat
     2281 aaacggtgtg ttgggccacg agcatgtacc actggcaaaa aaacaggcgc gcagtttagc
     2341 ataagcgctc cggcgcaggc acgccggagc agtgggcatt atttgggaag cgcagttccc
     2401 cattcacgcc actctttcgg ttcactttcc tgcaacagga agtgtttgcc ttcttgggct
     2461 ttcggtgcaa gtggcgtacc cggaggcgtg gcgaaggcga tgccgccacg gataaattgg
     2521 ttaaaggtgc cggttttcac tacgccgccc gtcagaccaa agtccagact gtaacccgat
     2581 gccaaccaga agacggaatt gttacgcacc aggtgttgat agcgtttact gatgcgcatc
     2641 gcaatcatca cgcgatctga caatgtcccc agcgtcattc cggtaaccgt acctacttcc
     2701 agaccacgga acagcacagg cgtaccgatg cctaacgaac cggcttccgg cgcttcaaca
     2761 ataatgctta aaccatctag gtaacgcgaa tcagtaatgg tggcctcttc taattcaaag
     2821 tcgcgacgag gattgccacg gcctggttcg acgttgatat acggctggag gatagtatct
     2881 agatgctcaa cgccagctgc cgagatttgc ggtgtgacca ctgagaagcg cgtaccaccg
     2941 cgagcaaagg tctggacata ttccggatag agcaccgcct ttgcctgtac ttcattgcgt
     3001 gcggtaatca gatccagcgt ctggatttgc ccgatatcaa taccgagata gcgaattggc
     3061 atcccgaccg ccagttttcc ggcatcgaaa gcgtgaagcg taatctgcct gccaaccgca
     3121 cgggccgctg tttcggaagc atacagaatt cgcttgtcgc ctttacgctg actggcgctg
     3181 gcaccactga ggttatcgaa gctaatggcc ccctttaatg ctctggagag cggggatgcc
     3241 tgtacggtca ggccactacc attcagctga actttcgccc cgccttctgc ccagaacacg
     3301 ctgttgctgg tcaaaaggtt gcgatattcc ggtttaatgt gcagatcgat atcgaacgcg
     3361 ttagctcgcg gacgcacggt aataacttca ccaacttcaa atttacggta cagcactacc
     3421 gatcctgcct gcacatccgg cagcgtctct gcactcaaac tcatggttgt ggtgggtaaa
     3481 tcgctaaggc tgttctccag cgctttttcc agattggcat acagtggata gctggctttc
     3541 atctcgcctt tatcgcccgg cagaatacgt atcccgccgt taatccattc tgaggcgctg
     3601 gcaccgagaa actcaacgcc atccagcccc accttcacgt cgacacggct gttgacgaca
     3661 aatttgctat cgccttttac cagttctcga tgctgaggct cgatggcgac ggtaaaggtg
     3721 acgcctttgc tggtgagttt acgatcgata acctggccta cctgcacgcc gtgaagaatg
     3781 agcggctgac ccgcatcaat accgtaactt tccggtgctg tcagggtcag tgtcagaacg
     3841 tccggctctt gcagcagggc tttttcgccc ggcacaacaa cgaactcttt gcgtggctcg
     3901 ccatcgccgg gcaccaactc aaaggttttt ccggtcagca gggcgctgag attagcatcg
     3961 ctaagggata atttcgggtt gcgtaattcg atgcgggtat tttcacgaag cagggatcaa
     4021 cggtcatttc cccagtgact ttaccaccag gatttaaatc cagtttagtc agctgtccga
     4081 cttccagccc ctgatacatt aacggcgtcg agtcggcggt taatcctgct ccactcggca
     4141 gttccagttt tattattact ccacgctggc tgtgggccag atcttcatac agaccaaagg
     4201 tatcttccgc ctcggcaggt ttcgactctt caggtgaatc gaaggcaatc gcaccgttaa
     4261 ccagtgccgc cagactttcc agtttcacct tcgcgccact gatactgacg ttggcatcaa
     4321 cgccggaaac gtttcagaaa cggctacctt ttttcaccag atcggtaaaa cgccgctcga
     4381 tcaggacatc aatcaccacg ccttgcttgt tgggattgat ggcatagtcg tagacttttc
     4441 ccaccgggat cttgcggaaa tagaccaatg aaccgctgtt cagcgaaccg agagcggggg
     4501 cttgcaggtg gatcatcaga tcgccattgt ccagccgata tttcggttgg gtatcgagcg
     4561 cgacaaagtg atcctgctct ttacctttac ccggcatcat gccgatatag ttcccaccga
     4621 cgagggcgtc cagcccggag acacctgcca acgatgcttt tggcgtcatc agccagaact
     4681 gagtctcttc gcgcagcgca tctttcatat cggacttgat gctgaccttg acttcaatct
     4741 tacgaagatc gtcgctgagg ctgatatcct gcactgttcc gacttcaacg ccctgataac
     4801 gaacaggcgt acggcccgga acaataccat ccgccgacat aaagtcgatg gtgacggtat
     4861 taccccggtc ctgataactg tcccaaatca gccaaccggc aatcattagc gcgatgaaag
     4921 gcagcagcca gaaaggtgag atacggcgtt tatttttaat ctgcgcttca gtcgtcgaag
     4981 cgggcgtttc ctgactcatg tgcatcccaa agtaagcggc tgtccagcca ttccacagca
     5041 agaatagtca aaattacggc agcgccaaaa taaaacgcag ccggtcccat agtaaaagcg
     5101 aggatctgat cgcgattaat cagcgacatg gttaaagata tgacaaacag gtcgagtatc
     5161 gaccagcgtc caatccaggt caccatccgc agtaacagaa tgcgtgtgcg taaaccttgc
     5221 tggcatttaa aatgaatgct caacagtaaa gtgaacatga cgatcacttt agtaaacggt
     5281 accagaatac tggcgataaa cacgattccc gcaaccgcaa tgttgctact tgccagcgac
     5341 ataattccgg aaagaatcgt atcttcctgc cggccaccat tcagataaat gatagaaata
     5401 ggcaataggt tggctggtaa caacaaaacg attgacgcta acagcgccgc ccagcatttt
     5461 tgcaaactat gacgacgacg caggcgtagc gggatatggc aacgcgggca gagaccacgc
     5521 tgatcaggat agccggtaaa atggcaccca agacagacac gaagtttctc gtccctacgc
     5581 gtagcggggc gctgcggata atatcgctcc cacagctcct caacattaag atgtgacaac
     5641 gtcaccgtcg ttaaaatcac caacgccaca aaagagaaca agccgacacc cgcctggata
     5701 tgggcataat cctgtacctt tatagaagca acgccaatgc cgaccaggta gatatccagc
     5761 attacccact ctttaagtcg ctcaagcatc agcagtaccg gacgtaaatt cattcccagt
     5821 cggttaccaa accataaata tgctatggag gacaccagaa tgaggggggc accaataacg
     5881 cagaaaaaga ccattgatcc cgttatcgca tcgccctgtt tggtcatttg ccagatgcct
     5941 tgcatgacgt tggcgtcaat acgaatgcct aacagccaga tatgcaacag cggttcgccc
     6001 caggcaaacg gcataagcaa cagcatggtg aaggccattg ccgccaggcg cgttagcgac
     6061 cagtcgcgcc cgtcgcgaat ttttgcctga cagcgcggac aataggcact ttgatgagaa
     6121 tttatctcgg gcaggctaaa cagcatgtca cattgcgggc aacgttggta atcaccacgc
     6181 ggcagttcct cgccgattgc cctcactgtt atcttttttg tcggcgtaat ttgtggtgtg
     6241 ttaagagcca tgacgtgcca aatgtaataa gaattaacat taatcttaac ccatgatggg
     6301 aatgatcttg agcattaacg catttaatgc ttattttaat aggctggcag ttccaggtaa
     6361 gacaaccaag tgattaagta atgaacaaaa cagaatttta cgcggattta aatcgcgact
     6421 ttaacgcgct gatggcggga gaaaccagtt ttctggcaac gcttgcgaac accagtgcgt
     6481 tgttatacga gcgtctcact gacgttaact gggcaggttt ttatttgctt gaggacgata
     6541 cactggtact cggaccattt cagggcaaaa ttgcctgtgt ccggataccc gtcgggcgcg
     6601 gggtgtgcgg cacagcggtt gcccgcaatc aagtgcagcg tatcgaggat gttcacgcgt
     6661 ttgacgggca tattgcctgt gatgcggcga gtaattctga aattgttctg ccgctggtgg
     6721 tgaaaaatca gattattggt gttctcgaca tcgatagtac tgtcttcgct cgctttacag
     6781 acgaggacga gcaaggctta cgtcagcttg tggcacagct tgaaaaagtg cttgcaacga
     6841 cggattacaa aaaattcttt gcgagcgtcg caggataatc aacggataac gtagcattta
     6901 ctggtagcgt cattataatg acgcctgttc atgcctgcgc ttgttggcta cgtccgttgt
     6961 aatcaggaaa tttcatggaa aatcaaccta agttgaatag cagtaaagaa gtaatcgcgt
     7021 ttctggccga acgttttccc cactgtttca gtgcggaagg tgaagcgcgt ccgctgaaaa
     7081 ttggtatttt tcaggatttg gtcgatcgtg ttgccgggga aatgaacctg agcaaaacgc
     7141 aattacgatc cgctttacgt ctctacactt cgagctggcg ttatctttac ggtgttaaac
     7201 ccggcgcaac gcgtgtcgat cttgacggca acccatgcgg tgagctggac gagcaacatg
     7261 tagagcatgc tcgcaagcag cttgaagaag cgaaagcgcg tgttcaggca cagcgtgctg
     7321 aacagcaagc gaaaaaacgc gaagctgccg cagctgctgg tgagaaagaa gacgcaccgc
     7381 gccgcgaacg caagccacgt ccgaccacgc cacgccgcaa agaaggcgct gaacgtaaac
     7441 ctcgtgcgca aaagccggta gagaaagcgc caaaaacagt aaaagcacct cgcgaagaac
     7501 agcacacccc ggtttctgac atttcagctc tgactgtcgg acaagccctg aaggtgaaag
     7561 cgggtcaaaa cgcgatggat gccaccgtat tagaaatcac caaagacggc gtccgcgtcc
     7621 agctgaattc gggtatgtct ttgattgtgc gcgcagaaca cctggtgttc tgaaacggag
     7681 gccgggccag gcatgaacat gttttttagg cttaccgcgt tagctggcct gcttgcaata
     7741 gcaggccaga ccttcgctgt agaagatatc acgcgtgctg atcaaattcc ggtattaaag
     7801 gaagagacgc agcatgcgac ggtaagtgag cgcgtaacgt cgcgcttcac ccgttctcat
     7861 tatcgccagt tcgacctcga tcaggcattt tcggccaaaa tctttgaccg ctacctgaat
     7921 ctgctcgatt acagccacaa tgtgctgctg gcaagcgatg ttgaacagtt cgcgaaaaag
     7981 aaaaccgagt taggcgatga actgcgttca ggcaaactcg acgttttcta cgatctctac
     8041 aatctggcgc aaaagcgccg ttttgaacgt taccagtacg ctttgtcggt actggaaaag
     8101 ccgatggatt tcaccggcaa cgacacttat aaccttgacc gcagcaaagc gccctggccg
     8161 aaaaacgagg ctgagttgaa cgcgcagtgg gacagtaaag tcaaattcga cgagttaagc
     8221 ctgaagctga caggaaaaac ggataaagaa attcgtgaaa ccctgacacg ccgctacaaa
     8281 tttgccattc gtcgtctggc gcaaaccaac agcgaagatg ttttctcgct ggcaatgacg
     8341 gcgtttgcgc gtgaaatcga tccgcatacc aactatcttt ccccgcgtaa taccgaacag
     8401 ttcaacactg aaatgagttt gtcactggaa ggtattggcg cagtgctgca aatggatgat
     8461 gactacaccg ttatcaattc gatggtggca ggtggtccgg cagcgaagag taaagctatc
     8521 agcgttggtg acaaaattgt cggtgttggt caaacaggca agccgatggt tgacgtgatt
     8581 ggctggcgtc ttgatgatgt ggttgcctta attaaagggc cgaagggcag taaagttcgt
     8641 ctggaaattt tacctgctgg taaagggacc aagacccgta ctgtaacgtt gacccgtgaa
     8701 cgtattcgtc tcgaagaccg cgcggttaaa atgtcggtga agaccgtcgg taaagagaaa
     8761 gtcggcgtgc tggatattcc gggcttctat gtgggtttga cagacgatgt caaagttcaa
     8821 ctgcagaaac tggaaaaaca gaatgtcagc agtgtgatca tcgacctgcg tagcaatggc
     8881 ggtggggcgt tgaccgaagc ggtatcgctc tcaggtctgt ttattcctgc gggtcccatt
     8941 gttcaggtcc gcgataacaa cggcaaggtt cgtgaagaca gcgataccga cgggcaggtg
     9001 ttctataaag gcccgctggt ggtactggtt gaccgtttca gtgcttcggc ttcagaaatc
     9061 tttgccgcgg caatgcagga ttacggtcgt gcgctggttg tgggtgaacc gacgtttggt
     9121 aaaggcaccg ttcagcaata ccgttcattg aaccgtattt acgatcagat gttacgtcct
     9181 gaatggccag cgctgggttc tgtgcagtac acgatccaga aattctatcg cgttaacggc
     9241 ggcagtacgc aacgtaaagg cgtaacacca gacatcatca tgccgacggg taatgaagaa
     9301 acggaaacgg gtgagaaatt cgaagataac gcgctgccgt gggatagcat cgatgccgcg
     9361 acttatgtga aatcaggaga tttaactgcc tttgaaccgg agctgctgaa ggaacataat
     9421 gcgcgtatcg cgaaagatcc tgagttccag aacatcatga aggatatcgc gcgcttcaac
     9481 gctatgaagg acaagcgcaa tatcgtttct ctgaactacg ctgtgcgtga gaaagagaat
     9541 aatgaagatg atgcgacgcg tctggcgcgt ttgaacgaac gctttaaacg agaaggtaaa
     9601 ccggagttga agaagctgga tgatctatcg aaagattacc aggagccgga tccttatctg
     9661 gatgagacgg tgaatatcgc actcgatctg gcgaagcttg aaaaagccag acccgcggaa
     9721 caacccgctc ccgtcaagta atatcaatca ggcacaagaa attgtgcctg attttttaac
     9781 agcggcaaga tgccgtaaat cagatgctac aaaatgtaaa gttgtgtctt tctggtgact
     9841 tacgcactat ccagacttga aaatagtcgc gtaacccata cgatgtgggt atcgcatatt
     9901 gcgttttgtt aaactgaggt aaaaagaaaa ttatgatgcg aatcgcgctc ttcctgctaa
     9961 cgaacctggc cgtaatggtc gttttcgggc tggtactgag cctgacaggg atacagtcga
    10021 gcagcgttca ggggctgatg atgatggcct tgctgttcgg ttttggtggt tccttcgttt
    10081 cgcttctgat gtccaaatgg atggcattac gatctgttgg cggggaagtg atcgagcaac
    10141 cgcgtaacga aagggaacgt tggctggtca atactgtagc aacccaggct cgtcaggcgg
    10201 ggatcgctat gccgcaagtg gctatctacc atgcgccgga catcaacgct tttgcaaccg
    10261 gtgcgcgccg tgatgcctct ctggttgctg tcagcaccgg tttgctgcag aacatgagcc
    10321 cggatgaagc cgaggcggta attgctcacg aaatcagcca catcgccaat ggtgatatgg
    10381 tcaccatgac gctgattcag ggcgtggtga acaccttcgt tatctttatt tcccgtattc
    10441 tggcgcagct tgccgcgggt tttatgggcg gaaatcgtga tgaaggtgaa gagagcaacg
    10501 gcaacccgct gatctacttt gcggttgcaa cggttctgga actggtgttt ggtattctgg
    10561 cgagcattat caccatgtgg ttctcgcgtc atcgtgaatt ccatgcagat gccggttcgg
    10621 caaaactggt tggtcgcgag aaaatgattg ccgcgctgca gcgcctgaaa accagctatg
    10681 aaccgcaaga agcaaccagc atgatggctt tctgcattaa cggtaagtcg aaatcgctca
    10741 gtgagttgtt catgactcac ccgccgctgg ataaacgtat tgaagctctg cgtacgggtg
    10801 aatacctgaa gtaatatgaa gtaaatacta aaaagcgcgt cctgatcgac gcgctttttt
    10861 tatgccctgg ctcgtggctg agtgatacgt aaaccactga cacaggcagc aatcactgcc
    10921 agaatagccg cagccatcag cgagacgtgc gtaccattat caccaaactg atttagcatc
    10981 agcgccacca gcgccgcgcc actactctga cccagtagac gagccgttcc taacatgcca
    11041 ctggccccac cgctacgttc gcgcggagcg gaggtaataa tggtgtggtt attgggtgac
    11101 tggaataagc caaatccagc gccacataag atcatcggcc agataatatt gatatccgca
    11161 ggtgacgcgg gcagcagaac cagggaaaaa agccccgtag ccatgatgaa caaccctaaa
    11221 gcacccagca atcctgcatg tacgcgttca atcaaatagc ctgccagagg agccatcacc
    11281 atcgttgcta acggccacgg tgtcagaagt aaacctgttt cgacttcact acgcccgagc
    11341 acggtttgca ggtaaaaggg cagggaaacc attgccagca tttgtgcgca gaaagagcaa
    11401 acagatgtgc aaatagaaag tgaaaacagc gggatacgca gtaaatccac cggtagcagc
    11461 ggtacgggaa gagaaagctg gcggcgaata aagaaaatac caacaacaac cattaccacc
    11521 agttccgcag caattaacgt cagcgattgc ccctgagcga aaccactcaa cgcagtgata
    11581 agcaggccga aggttaacgc gttcatcacg gcgctgggca ggtcgaaacg gggtttactg
    11641 gcgcgagaac cattgggtgg cagaaaacgc atcgccagaa gcagggcgat aatacctaac
    11701 ggtacgttga ttaaaaataa ccatttccag gatgcgatgg agaggattgc tgcagcaatt
    11761 gtcggcccgg cagcagaaga gacggcaaca ataaacgagt ttatgcccat ccctctcccc
    11821 agaaaacgtt gtggatagat caggcggata agtgcggtat taacgctcat caacgctgca
    11881 ccgccgaaac cttgtatgac acgcgcaagg gtgagcattt gcagcgaatc agaaagggcg
    11941 cagaacagtg aagacaacag aaaaacgacc agaccgcatt tataaatacg tcgatagcca
    12001 aacatatcgc ccagaaacga aaacgagagc agggagatga caatggcgat ttgataggcg
    12061 ttcactaccc agatggaact ggctggtgtg gcatgaaggt ccgtggcaat tgttggcagg
    12121 gcgacgttgg cgattgcgcc gtcaaggaca gccatcgaaa taccaatcac aatggttaat
    12181 atcgcaccgt atcgctgggg caatggcagg ccgtcggcct gaacttttgg cataggaatt
    12241 ttatatcttt ggtgaataat caacaggggt atgattctag cactgatatt cacggtggat
    12301 gtcgcagaat tgtaactaac cggcaaaaga tggattgcga ccaccctggt gcgaaattat
    12361 aataaaaaac agttctgatt tttataaaac actcgcaatg aggtgataaa tggctaacgc
    12421 agatctggat aaacagcctg attctgtatc ttccgtgcta aaagtttttg gcattttgca
    12481 ggcgctgggt gaagagcgcg aaatagggat aaccgagctg tcgcagcgcg tcatgatgtc
    12541 aaaaagcacc gtttatcgct ttttacagac catgaaaacc ttaggttatg tggcgcagga
    12601 aggggagtcg gagaaatatt ccctgaccct gaaattgttt gaactgggcg ctcgcgcgtt
    12661 acaaaacgtc gatttaattc gtagcgcaga tatccagatg cgtgagatct cccgcctgac
    12721 caaagaaact atccacctcg gcgcactgga cgaagacagt attgtttaca ttcacaaaat
    12781 tgactctatg tacaatttgc gcatgtattc acggattggg cgtcgtaatc cgctgtacag
    12841 caccgcgatt ggtaaggtac tgctggcatg gcgcgatcgc gatgaagtga agcaaattct
    12901 tgagggcgtg gagtataaac gcagtaccga gcggaccatc accagtacag aagcgttatt
    12961 acccgttctg gaccaggtgc gcgagcaggg gtatggagaa gataacgaag agcaggaaga
    13021 agggctgcaa tgcattgcgg taccggtatt tgatcgcttt ggcgtggtca ttgccggttt
    13081 gagcatctcc ttcccgacgt tgcgtttctc tgaagagcgt ttacaggaat atgtcgcaat
    13141 gttgcatacc gcagcgcgca aaatttctgc ccaaatgggt tatcacgact atccgttctg
    13201 atgagagtaa gaacctgtcg gaatatcaaa cagacaggtt ctttatttag catgagaaaa
    13261 ataaagttga aggtgcgtta tattaaacgc gcttgctata agaatatttt actcatgagt
    13321 gagaatctgg ttatttatta cccttaacca ttatcgacca cgatattgct tttacgtaac
    13381 agtgggcaat ctgttatctc caagaaacca cttttagtgt gcaagtattg taccgtgctg
    13441 gtgcctctgg cagtcagata ggtacattgc aaacctaatc ctgcggcatt ctctttgctt
    13501 ccaatcaaaa cgccatatcc gctgagtaat aatcctatcc ataccagtgc tatcagcata
    13561 actgtgcgaa tgatgaatcg cattacaatc tcttctcttt ttatgttcgc ttaatcgtag
    13621 cggcaatatg cgctgaagca agctactcat tccgaaaaag cacgaatatc gacatagtta
    13681 ggcgctgttt aactaacgca tgctagttta atgacataag gtaggtgaag cggagattgg
    13741 agtgaaaaag tttcgatggg tcgttctggt tgtcgtggtg ttggcttgct tgctgctttg
    13801 ggcgcaggta ttcaacatga tgtgcgatca ggatgtacaa tttttcagcg gaatttgtgc
    13861 cattaaccag tttatcccgt ggtgatatca ttttttcagc actagatttt cttcctctcc
    13921 agcagtggta gaatagcgcc tctactcaga ggtggtgaaa tgaacgaagt tgtaaattca
    13981 ggcgtgatga acattgcgtc tttggttgta tcggtggtgg ttcttcttat cgggctcatc
    14041 ttgtggtttt ttatcaatcg tgccagttca cggactaacg aacagattga actgcttgag
    14101 gcgttgctgg atcagcaaaa acgtcaaaat gcactgttac gtcgtttgtg cgaagcaaac
    14161 gaaccagaga aagcagataa aaagaccatt gagagtcaaa aatcggttga tgacgaagat
    14221 attattcgcc tggtcgccga acgataatcc ttcctcaatc ttgagtaacc ccgtcgttga
    14281 agggttactc aattcatcct gattatacgc actgtcatgg tgcaactgct tcaaattaaa
    14341 actctggtct gacttcaaat aattatcata ttatttattt gcgtaacggg atatcagaaa
    14401 aagatatttt cctcattaat ttattaggat gtttacatca gatttgtgat taagcgtggt
    14461 atta
//
LOCUS       DAZPCS010000092        14427 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZPCS010000092 DAZPCS010000000
VERSION     DAZPCS010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14427
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.092"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..132)
                     /locus_tag="ACHUQ7_002672"
     CDS             complement(<1..132)
                     /locus_tag="ACHUQ7_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFR1136024.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     "
     gene            315..458
                     /locus_tag="ACHUQ7_002673"
     CDS             315..458
                     /locus_tag="ACHUQ7_002673"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1136025.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
     gene            complement(677..2215)
                     /gene="aldB"
                     /locus_tag="ACHUQ7_002674"
     CDS             complement(677..2215)
                     /gene="aldB"
                     /locus_tag="ACHUQ7_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HFR1136026.1"
                     /translation="MTNNPPSAQIKPGEYGFPIKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(2380..3531)
                     /gene="yiaY"
                     /locus_tag="ACHUQ7_002675"
     CDS             complement(2380..3531)
                     /gene="yiaY"
                     /locus_tag="ACHUQ7_002675"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HFR1136027.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVVIYRAAM"
     gene            complement(3721..5565)
                     /gene="selB"
                     /locus_tag="ACHUQ7_002676"
     CDS             complement(3721..5565)
                     /gene="selB"
                     /locus_tag="ACHUQ7_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFR1136028.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHA
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            complement(5562..6953)
                     /gene="selA"
                     /locus_tag="ACHUQ7_002677"
     CDS             complement(5562..6953)
                     /gene="selA"
                     /locus_tag="ACHUQ7_002677"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFR1136029.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLVAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(7051..7659)
                     /locus_tag="ACHUQ7_002678"
     CDS             complement(7051..7659)
                     /locus_tag="ACHUQ7_002678"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFR1136030.1"
                     /translation="MKLVGSYTSPFVRKLFILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(7730..8815)
                     /locus_tag="ACHUQ7_002679"
     CDS             complement(7730..8815)
                     /locus_tag="ACHUQ7_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFR1136031.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHPYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(8818..9180)
                     /locus_tag="ACHUQ7_002680"
     CDS             complement(8818..9180)
                     /locus_tag="ACHUQ7_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFR1136032.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATEPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            9717..11630
                     /gene="mtlA"
                     /locus_tag="ACHUQ7_002681"
     CDS             9717..11630
                     /gene="mtlA"
                     /locus_tag="ACHUQ7_002681"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFR1136033.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGKRGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            11725..12873
                     /gene="mtlD"
                     /locus_tag="ACHUQ7_002682"
     CDS             11725..12873
                     /gene="mtlD"
                     /locus_tag="ACHUQ7_002682"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFR1136034.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            12873..13460
                     /gene="mtlR"
                     /locus_tag="ACHUQ7_002683"
     CDS             12873..13460
                     /gene="mtlR"
                     /locus_tag="ACHUQ7_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFR1136035.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAVENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(13472..13681)
                     /gene="yibT"
                     /locus_tag="ACHUQ7_002684"
     CDS             complement(13472..13681)
                     /gene="yibT"
                     /locus_tag="ACHUQ7_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFR1136036.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            13965..14327
                     /locus_tag="ACHUQ7_002685"
     CDS             13965..14327
                     /locus_tag="ACHUQ7_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFR1136037.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTTF"
BASE COUNT         3529 a         3843 c         3623 g         3432 t
ORIGIN      
        1 aatggcgtcg gcatgaggat gattacgttt tttagcaatc aggtagggaa tatcatgtaa
       61 aataataatc ccatagaata tcaccagaaa tacaaaaata agcactccca gtgcaaaata
      121 gtccaggaac atatttccct caaagaatat aaaaaagaac aattaacgca tattatgaaa
      181 tgccatgatg cagtgcaatt tccttattta tataacgaca tattgttttt tatatacttt
      241 ctgaaatgtg ctgtctggtt tttcaacagg acgaggttta tcatattgaa atagtggaac
      301 tattggccaa actaatgaat aactccagta aaacatccac agtacagatt aagcgtatta
      361 aaccttcaat tatctaccgt ttattgctga ttggcctcgg atcaccaatg gtgatttacg
      421 gcctggttcg cccgctcacc atcgaaacgc gagattaaac tcctgacaaa acacgcccca
      481 gaaacgcact tctgttggcg tagtctgggt tattgcgcac gtaggtttaa aacgtaaatt
      541 gtaacaacgt gcgctttgtt tatgccggat gcggcgtgta cgccttatcc ggcctacggt
      601 caggttcccg taggcatgat aagacgcgta agcgtcgcat caggcaatga atacccaatg
      661 cgaccagctt cttatatcag aacagcccca acggtttatc cgagtagctc accagcaggc
      721 acttggtttg ctggtaatgc tccagcatca tcttgtgggt ttcgcgaccg atacctgatt
      781 gtttgtagcc accaaacgca gcatgtgccg ggtaagcgtg ataacagttg gtccacacgc
      841 gcccagcctg tatgccgcgc cccatcttat aggccagatt accgttgcgg ctccagacgc
      901 ccgcgcccag gccatattgc gtatcgttcg ccagctccag cgcttcttcc atcgttttga
      961 aggtggtcac cgccagcacc gggccaaaaa tctcctcctg gaacacccgc atattgttct
     1021 gaccaaacag aatcgtcggt tcgaggtagt agccgtcttt cagttcacct tccagcagct
     1081 tgcgccgccc gcctgtgagc acgtcagcgc cctctttttt accgatatca atgtagttga
     1141 ggatggtttc cagttgcccg tgagaaacct gcgcgcccat ttgcgtcacg ctgtcgagcg
     1201 ggttaccgct gcgaatgctt tcgacacggc ggatggcgcg ttccataaag cgttcgtaga
     1261 ttgactcctg cactaaggca cgactcggac aggtgcaaac ttcgccctgg ttaaaggcaa
     1321 acagtgcaaa gccttccagc gctttatcga aaaaggcatc ttcttcatcc atcacatcag
     1381 caaagaagat atttggcgac ttaccgccca actccagcgt caccggaata atgttttgcg
     1441 ttgcgtattg cataatttgt tggcccactt ccgttgagcc ggtaaacgcc actttggcga
     1501 tgcgtttcga ggtcgccaga tattcgccaa tttccccacc tgcgccattg accacgttca
     1561 ccacgcccgg cggcagtaaa tcaccgacaa tttccattag cagcagtaca gaaagcgggg
     1621 taagacgtgc gggtttcagc accacacagt tgcccgccgc cagcgcggga gccattttcc
     1681 agctcgccat cagcagcggg aagttccacg ggataatctg ccccaccacg cctaacggtt
     1741 catggaaatg ataggccacg gtttcgctat caacttcact gatcccacct tcctgcgccc
     1801 gaatacacga ggcgaaatag cggaaatggt caatcgccag cggtacatcc gcagcactgg
     1861 tttcgcgaat gggtttgccg ttatcccagg tttcagctgt cgctaacagc tcgaggtttt
     1921 gttccattcg atcggcaatc ttaaacagaa tcgccgcgcg atcctgcacc gaggtgtgcg
     1981 cccatttatc tttcactttg tgcgcagcat ccagcgccag atcgatgtct cgtttgcccg
     2041 aagacgccac ttcgcacagc agctgcccgg tcaccggcgt cagattctgg taatactcac
     2101 catcggcagg ggctacccat tcaccgccaa taaagttgtc gtagcgggct tttaacttga
     2161 tggggaaacc atactcgccg ggcttaatct gtgctgaagg gggattattg gtcatgatcg
     2221 tctccttgcg gtgtgaggta taacaagggt agacgtgact ggcgaaatct tcgccagtcg
     2281 gtaacagctt tacgacagct atcacgaatt tacgggcaag gattacagag atagttgaaa
     2341 aagcggctga caatttgcca gccgttgttg gaatgatgat tacattgcgg cgcgataaat
     2401 caccacaatt tcttcgtgag ttgcctggat cgggttagta aagccacagg catctttcag
     2461 ggcattagtc gccagaaccg cgaaatcttc ttctttcacg ttcaggtcgc gtaggcctgc
     2521 cgggatatcc actttcttcg ccagttcacg gatggcgtta atgcaggctt cagcaccttc
     2581 cgcgtcgttt ttacctgtca cgttcacgcc cattgcagcg gcacagtcac gcagacgtgc
     2641 agcggcgact ttgctgttga atacctgaac atgcggcagc agaacggcgt tacatacacc
     2701 gtgtggcagg ttgtagaaac cgcccagctg gtgcgccatc gcatgaacat aacccagaga
     2761 agcattattg aacgccatac cggcaaggaa ctgggcataa gccattgctt cacgcgcttt
     2821 cgcattactg ccatcttcaa cggctaacgg caggttttcg gcaatcatgg tcacggcttt
     2881 cagtgcacaa gcgtcagtga tcggcgtggc ggcaatagaa acatacgctt cgatagcgtg
     2941 cgttaaggca tccatacccg ttgcggcggt cagtgacttc ggcataccaa tcatcagaga
     3001 ggagtcattg acagaaagca gcggagtcac gtgcttatca acaatcgcca ttttgttatg
     3061 acgcgcttcg tcagtgatga tgcagaaacg ggtcatttca gaggccgtac ccgccgtggt
     3121 attgatggcg atcatcggca gctgcggttt tgcagagcgg tcaacgcctt cgtaatcgcg
     3181 aatatcgccg ccattggctg ccaccagcgc aatacctttt gcgcagtcgt gtggagaacc
     3241 accgcctaag gagatcacgc tatcgcaatt attttcttta agtaatttca aacctgcggc
     3301 gacgttttcc gtggtggggt taggttgggt gccatcataa ataacgctaa aaatattgcg
     3361 ttcttccagt gctttttgca catcacccgc catacctaat ttcgttaaca tattgtcagt
     3421 gacaattaag gtacgggtaa atccataatc tgccatcata ttcattgcat cagtcaatga
     3481 atcagcgccg atgacattca cagaaggaat aaagaaagtt gaagatgcca taacacactc
     3541 ctgaaagtgt aaaaaagggt gggcagctta ctgaggattt tcattcgaaa acatgatcat
     3601 tattattaat tatcaaaatt aatctaataa aaagcacatt gtattcatta aacaatgtgc
     3661 tttatttaaa atcataagaa atttgtatta atttatttta aaaattaatt aatcatttcc
     3721 ttatttttcc ggaaataata atgcgtcgcg caataaatga tcatttccac gacgacgcgt
     3781 aaagccaatg cggtcaaaat attccagaat ctgaattgcc agctttcggc ctacgcctaa
     3841 gcgatcgcgg aaatccgccg cgcaggttga accacactcc tgatcgagat cgcggatcat
     3901 attggcaaat tcgacaatcc gatcgttacg gtaataacga tctttgacga tcgcggtaat
     3961 tattccttgc tgcgccgcct ggcgtagcgt caggcgcatt gcctgctcgt cggtacccgt
     4021 ctcttttgcc agatcacgca cccaccacgg ctcgtcacca aacagcggct ctgctttttg
     4081 ccagatggcc tgctgctctt cgctgaagcc cgctttgtga tccggcagat gcagccaacc
     4141 gtgatgactg aggatgtcgc cgctttcgcg catcttttca atcagcaaca gtactagcgc
     4201 ttcatcttcc attggcaacg ccatacgtcg cagacgttcg cgcccaggcc caggttcatc
     4261 gcgatgttgc tcatgataag tcgctaatgt gtcgagaatt ttccgttgcc agcgagcggc
     4321 aaccggcgca ttcaacaagc tataaccagc ctgaatatag ccaggctgtt gcagcagttc
     4381 gcgcatccct tcgccgttga gctggcgcgc ccaagcgaaa tcaggaagat taaccgcgcc
     4441 gcgttgcagg tgaacggcta acgcttcgct atcattctgt gccctggcga gggaagccag
     4501 ccattgcaga tactctggtt tacgtttacc gcgacgcggc gggttaagca tcacgacgcg
     4561 cgctccggcc agcgtgttgc gggcagagat atcgcgcaat accaggcggt cgttatctgc
     4621 cagccataac ggggtgtcga agaccagttc agcaaggtta tcttccagca gtgaaacgcg
     4681 tcccgtgacg tggctggcgg cgtggtgaat atgcagcggc tgccactggg tcagcggtgc
     4741 atgggtttga agctcgacaa tcacccgtgt gaacggctct ggcggcacat cggcaagcag
     4801 ccagtcgcca cggttaatct gctctttttc cgcatcaccc gcgatgtcaa gcgcgatacg
     4861 ctgcccggca ttggcggttt ctgttggctg gttttgcgca tgcagcgcac gtacacgcat
     4921 cggtttattt acaccagtca gccagagtga atcgcctacc ttcacttccc cgcttaacgc
     4981 cgtaccggtg acgaccagcc cggcaccttt tacggtaaat gcgcggtcaa tcgcgaggcg
     5041 gaaactatgt tggctggcgt gctcgcgttc cggcaactga agcagatgct cgcgcagggc
     5101 atcaattccc cgaccttcgg ttgctgcggt gataaacagt tttgcctcag caaaaccgta
     5161 ttcccgcaga acctccttta cctggcgttc aacctcatca acacgcgctt cgtccacgcg
     5221 atcggctttg gtcagcgcca ctgtcagcat cgggttaccg gtcagctgca aaatcgccag
     5281 atgctcacgg gtctgtgcca tcacgccgtc atcgcacgcc accaccaaca gcgcgtgatc
     5341 gataccacca acgcccgcca gcatgttgga aagaaacttt tcatgaccgg gaacgtcgat
     5401 aaaaccaggc acgcgaccat ccggctgcgg ccagtaggca tagccgagat cgatggtcat
     5461 gccgcgcttt ttttcttccg gcagacggtc agcatttacg ccagtaatcg cctgcaataa
     5521 ggttgttttg ccgtggtcaa cgtgtccggc agtcgcaata atcatttcaa caacatctcc
     5581 aaaaaccgtt gctcatcttc aaggcagcgt aaatccagcc acaatcgtcc gtcataaata
     5641 cgaccaatca ccggcactgg caattcacgc cagcgggcgg ctaatgactc aaggtggcta
     5701 ccgcgtccat catggggtgt aaacgttaat gccgcgctcg gcaggcgatc aaccggcagc
     5761 gaaccactgc caatctgcga aagacatggc ataacctgta ccgcaaactc cgcgccgtaa
     5821 tgtgcgacaa ggggagcctg taaacgttgt gcctggattt gaatgacctc tgcgctgcgg
     5881 gtaagcaggc gcagggtcgg taatttttca ctcagagctt cagggtgtaa ataaagacgc
     5941 aacgtggctt ccagcgccgc gagggtcatt ttatccgcgc gtaatgcacg cttcagcggg
     6001 tggctttgca ggcgggcgat catctctttt ttaccaacga taatcccggc ctgcggcccg
     6061 cctaacaact tgtcgccgga gaagctcacc agactgacgc ccgccgcaat caactcctgc
     6121 ggcattggct ctttcggcaa accgtactgg ctaagatcga ccagcgagcc actgcctaaa
     6181 tcagtcacca ctggaacatc cagctctttg ccgagcgcca ccagttcagc ttcgtctatc
     6241 gctttggtga acccctgaat gctgtagtta ctggtatgta ctttcatcaa cagtgcggta
     6301 ttttcattca ccgcctgacg ataatcattc gcgtgcgtgc ggttggtggt ccctacttcg
     6361 tgtagggtgc agcctgcctg acgcataaca tcgggaatac gaaacgcgcc gccaatctcc
     6421 accagttcgc cgcgagatac caccacctct ttcccgctgg cagtggccgc caacatcaat
     6481 aacaccgccg ccgcattgtt attgacgata caggcatctt ccgcccccgt aatacggcac
     6541 agcagctgcg ccagcgcccg atcgcgatgt ccgcgtccgg cgtcgtccag atcgtactcg
     6601 agggtcactg gcgaacgcat agcctgcgca acggcttcca ccgcggcttc cgcctgtaaa
     6661 gctcgcccaa ggttggtatg cagcacggtt cccgtcaggt tgatcaccgg acgcagcgcg
     6721 ctctgcgcct ctttcgtcaa ccgggcatcg acttcttgcg cccagttttc acaccacgca
     6781 ggcagcgtct ggctgccacg aatcacttct cgcgcttcgt cgagcatctg acgcaacaat
     6841 tccaccacgc gggtgtgacc ataagtatca cgcaaagaaa ggaaggagct atcgcgcaat
     6901 aagcgatcaa tagccggaag ttgactatag agggaacgcg tttcggttgt cataggaaac
     6961 ctggctgatc aaggccctct cacacggaga agggcgttta acataaccac ggattgtaac
     7021 gtgagatggg tcaggaggac atatcgcgca tcaagccttt ggcggttcgg tgcgagcaaa
     7081 actttcgcgg ctaaacaggt tttcgaccag tttgactaag tgtgggcggt cgacacacca
     7141 acctggcgca acgcggcgga aattgagata tccgacagca caggcaatgg cgatagtcgc
     7201 cagattgacc gtatcggttt tgagtgtgcc atcgaccaga tatccttcca gcacatccag
     7261 actgcggttg attttctccc gctggcgtaa caattcatct tcagactgct gcgccgccgg
     7321 acgcgcctgt tcacgcaccg ataccagccc ggcatccata atgccatccg ccagcgcctc
     7381 aattttgcgc acccgcaacg actccagcgg atcgcgcggc aacatcgccg gagcgacatt
     7441 cattaactca atatattcag cgatgatcgg cgaatcaaac cagcattcgc cctcttcggt
     7501 caccagcacc ggcactttcc ctaacgggtt aaattgcgcc acgccgttat ccgcgttata
     7561 gggcagttca ttaataaatt cgaaagttat gcccttttct aacaacagaa taaaaagttt
     7621 gcgtacaaac gggctggtgt agctaccgac gagtttcatg ccgagtcctt tgtgcgagga
     7681 aaaatatcag tatggctcag gctcacgcaa aacacaggtg atataagtct caatgatcca
     7741 aataaagata atgcatccaa ctggtcattc ttagcagcac tttccgcatc accgaaacat
     7801 ggctgaaatg gtcggagtaa accgccacct gggcgtagat gccgtcgggt aaggcatcga
     7861 tatcatcgtt agggtccagt tcaatggttc ccagcacacc gtccgtgccg ggaacgaccg
     7921 ttaatgattg caataccccc tgcgcctgat aagaaccgcc tggcacgaca ggtaaaatac
     7981 tagtcaattt gccgtggaac acctgcccag gtagcgcgtt aaacaccact tccgcatcat
     8041 cgccaggttt cagacgtaac agcgagtttt gccgaaattg ggcgacaatt tgccgttttt
     8101 gctcggggat gaagaccatc accggacgca gcggcaaggc agctgcgtat gtacctgggc
     8161 ggatcagtac ctgagtgacg tagccattgc ttggtgcgcg aatgacagtc tgctcaagat
     8221 tatattttgc ttcagtaagt tgcgctctta agctcacaat ctgcgattgc tcgccgttga
     8281 ccatactgtc gagctgactc tggatctgcg cctgctccgc caccgagcct ttcactaacg
     8341 catcctgcgc gaggaaattt tgccgcgcat cgtcgatgtc acgttccgag aacggattca
     8401 ccgccgcctg gctgcctttc aggtaacgtt gataattttt aaacagacgg tcgcgctccg
     8461 ctgaaacctg ggtggtgttg gcctgcgctt cagtgagctg cgcacgcagc gtctttatat
     8521 tatgcgtcgc cgtcatcagg tcagcctgaa gtctgtcaac tcgcgcctgg taacgaaccg
     8581 ggtcgagctt aaaaagcacc tcgccctttt gaataagctg attattcttg tcagtgactt
     8641 cagtaacaat tcccgtcacc tgtggcgtga tagggatcgc tatcactgcc ttttgcgcgg
     8701 taaaagtgta agggtggttg tagttcatca acaaaatcaa accactcacc agaaacacgc
     8761 ctcccagcgc cgccgtcgcc agcgccacgt aagttaaaac aatcaataga tccataatta
     8821 gatctccggg aacgtggaat agtcagtctt tttctcggcg gcgagttgat gctccagccg
     8881 ggaaattcgg tcagaaagtg cggcgatttc cggttcagtc gctttctcct gcgacgcaac
     8941 atgtgactgc ataccccagc cacgctccgg ttgatagagc gtcgcccaga tccacagaaa
     9001 cggccagata acatgcagag taaacaggct cacccagccc gccgtatgaa tagcgtcggc
     9061 atgggggtga ttgcgctttt tggcaatcag ataagggatg tcatgtatcg cgatgattcc
     9121 ataaaaaatc accagaaaga cgaagatcag cactcccaac gcgaaatagt ttagaaacat
     9181 atctgcctcg gattcacgtt tatcagtgtt gttttttggc tggcagccag aagggagtca
     9241 ggctgatatt ttgacaataa tccgggttcg cgattctcgc cataacacca aagaataatt
     9301 tttagaggtg atgagttgct tagttacata acgattgtat gacgaaggca taacatgctg
     9361 tagatcacat caggtgaacg ccgtaagaaa atatcttgtg attcagatca caaagattca
     9421 acaaaccatc aaaacaaaaa tgtgacatta ctcacattta aatgccattt ttagcgaaaa
     9481 tcaccgcctt gtttcttttt tatacaggcg ttttgtgatg agcgtcacgt caattacctc
     9541 tctaccccct atatttatgt gactgatatc acacaaaagc ccgtcgactg gacagttaac
     9601 cgattcagtg ccagatttcg cagtatctac aaggtccggc tacctctgcc gccacattaa
     9661 caaaaaacct cgggcttcca gcctgcgcga cagcaaacat aagaaggggt gtttttatgt
     9721 catccgatat taagatcaaa gtgcaaagct ttggtcgttt cctcagcaac atggtgatgc
     9781 caaatatcgg cgcgtttatc gcgtggggta tcatcaccgc gttatttatt ccaacagggt
     9841 ggttaccgaa cgagacgctg gcgaagctgg tcgggccgat gatcacttat ctcctaccgc
     9901 tgctgatcgg ttataccggt ggtaagctgg taggcggcaa acgtggtggc gtagtcggtg
     9961 ccattaccac catgggcgtt atcgtcggcg cagacatgcc gatgttcctc ggttctatga
    10021 ttgcaggtcc gctgggcggc tggtgcatta agcacttcga ccgctgggta gacggtaaga
    10081 tcaaatccgg ttttgagatg ctggtgaata acttctccgc aggcatcatc gggatgatcc
    10141 tcgctattct ggcattcctc ggcattggcc cgattgttga agccctgtcc aaaatgctgg
    10201 ctgcgggcgt taacttcatg gttgtccatg acatgctgcc gctggcgtct atctttgttg
    10261 aaccggcgaa aatcctgttc ctcaacaacg ccattaacca cggtatcttc tcgccgctgg
    10321 gtattcagca gtcccatgaa ctgggtaaat caatcttctt cctgattgaa gctaacccag
    10381 gtccaggtat gggcgtgctg ctggcgtaca tgttctttgg tcgtggtagc gctaaacagt
    10441 ctgcgggcgg tgcggcaatc atccacttcc tgggtggtat ccacgaaatc tacttcccgt
    10501 atgtgctgat gaatccgcgt ctgatcctcg cagtcatcct cggcggtatg actggcgtgt
    10561 tcacgctgac tatcctgggc ggtggtctgg tttctccggc atctccgggt tctatccttg
    10621 ctgtactggc gatgacacca aaaggtgctt atttcgctaa catcgcgggt gtgtgtgcgg
    10681 cgatggctgt ctccttcgtt gtctctgcta ttttgctgaa aaccagcaaa gtgaaagaag
    10741 aagatgatat tgaagcagca actcgtcgta tgcaggacat gaaagctgag tctaaaggca
    10801 catctccgct atctgcgggc gatgtgacta acgacctgag ccacgtacgt aaaatcatcg
    10861 ttgcctgtga cgccggtatg ggttcaagtg cgatgggcgc aggcgtgctg cgtaagaaaa
    10921 ttcaggatgc aggtctgtcg cagatttcgg tcactaacag cgcgatcaac aacctgccgc
    10981 cagatgtgga cctcgtcatc actcaccgtg acctgaccga acgcgctatg cgccaggttc
    11041 cgcaggcaca gcatatttcg ctgaccaact tcctcgacag cggcctgtac accagcctaa
    11101 ccgaacgtct cgttgctgcc cagcgccata ctgaaaacga agtgaaagtg aaagacagtc
    11161 tgaaagacag ctttgacgat tccagtgcta acctgttcaa actgggcgcg gagaacatct
    11221 tcctcggtcg caaagcagca accaaagaag aagcgattcg ttttgctggc gagcagctgg
    11281 tgaaaggcgg ttacgttgag ccggaatacg ttcaggcgat gctggatcgt gaaaaactga
    11341 ccccgactta tctgggtgag tctatcgcgg tgccacacgg tacggttgaa gcgaaagatc
    11401 gcgtactgaa aacgggcgtg gtgttctgcc agtacccgga aggcgtgcgc ttcggtgaag
    11461 aagaagatga cattgcccgt ctggtgattg gtattgcagc ccgtaacaac gagcacattc
    11521 aggttatcac cagcctgacc aatgcactgg atgatgagtc cgtcatcgag cgtctggcac
    11581 acaccaccag cgtggatgaa gtgctggaac tgctggcagg tcgtaagtaa tccaatccta
    11641 ccctctcctc acggagaggg tttgggtgag ggaaaagcct caccccagcc ctctcgggta
    11701 aaaacattga tgaaggttaa tactatgaaa gcattacatt ttggcgcagg taatatcggt
    11761 cgtggcttta tcggtaaact gctggcagac gcgggtatcc aactgacgtt tgccgatgtc
    11821 aatcaggtgg tacttgatgc cctgaatgcc cgtcatagct atcaggttca tgtggtcggt
    11881 gaaaccgagc aggtagatac cgtttccggc gtcaatgctg tcagcagcat tggtgatgat
    11941 gtcgttgatc tgattgctca ggtcgattta gtcactaccg ccgttggccc ggttgtgctg
    12001 gaacgtattg ctccggctat cgccaaaggg ctggtgaaac gcaaagaaca aggtaatgaa
    12061 tccccgctga acatcatcgc ctgtgaaaac atggtacgcg gcaccacgca gctgaaaggt
    12121 catgtgatga acgccctgcc ggaagacgcc aaagcgtggg tagaagagca cgttggcttt
    12181 gtcgattccg ccgttgaccg catcgtaccg ccttcggctt cggcaactaa cgatccgctg
    12241 gaagtgacgg tagaaacttt cagcgaatgg attgtcgata aaacgcagtt caaaggcgca
    12301 ctgccgaaca tcccaggcat ggagttaacc gacaacctga tggcatttgt cgaacgtaaa
    12361 ctcttcaccc tgaacacggg tcatgctata accgcgtacc tcggaaaact ggccggtcat
    12421 cagaccattc gtgacgctat tctcgacgag aaaatccgcg cggtggtaaa aggtgcgatg
    12481 gaagaaagtg gtgcggtact gatcaagcgc tacggctttg acgcagacaa acatgcggcg
    12541 tacatccaga aaatcctcgg tcgttttgag aacccgtatc tgaaagatga tgtagagcgc
    12601 gtaggccgtc agccgctgcg taaactgagt gctggcgacc gtctgatcaa gccactgctc
    12661 ggtacgctgg aatacagcct tccgcacaag aatctgattc aggggattgc tggtgcaatg
    12721 cacttccgca gtgaagatga tccacaggct caggaactgg cagcactgat cgctgacaaa
    12781 ggtccgcagg cggcgctggc acagatttcc ggtcttgatg ccaacagcga ggttgtatcc
    12841 gaggcggtaa ccgcttataa agcaatgcaa taatggtgga ccaggcgcag gacaccctgc
    12901 gcccgaataa cagattgtca gatatgcagg caacaatgga acaaacccag gccgttgaaa
    12961 accgtgtgct tgagcgtctg aatgctggca aaaccgtgcg aagctttctg atcaccgccg
    13021 tcgagctttt gaccgaggcg gtaaatcttc tggtgcttca ggtattccgc aaagacgatt
    13081 acgcggtgaa gtatgctgta gaaccgttac tcgacggcga cggtccgctg ggcgatcttt
    13141 ctgtgcgttt aaaactcatt tacgggttgg gcgtcattaa ccgccaggaa tacgaagatg
    13201 cggaactgct gatggcattg cgtgaagagc taaatcacga cggcaacgag tacgccttta
    13261 ccgacgacga aatccttgga ccctttggtg aactgcactg cgtggcggcg ttaccaccgc
    13321 cgccacagtt tgaaccagca gactccagtt tgtatgcaat gcaaattcag cgctatcaac
    13381 aggctgtgcg atcaacaatg gtcctttcac tgactgagct gatttccaaa atcagcttaa
    13441 aaaaagcctt tcaaaagtaa gcaacgtctg cttactgccc ctctacctgc ttcggccgat
    13501 aaagccgacg ataatactcc agacgttgta gatataacgg cacgctttca tcgggtattc
    13561 cggacggaat cgcgttacgg ggaggaagtt ttttcagata ctcccggaac gcctggcttg
    13621 atgccatgaa atctacggct ttatcgataa gaagcggaac gttttcacct aatttgccca
    13681 tgatgatatc tccgtattac cccgcgccgg gaatgcgcgg ccgccaattt tagtttaggt
    13741 tcccggtaaa gatgacatta aggaaaacgt gctgaatcct caataatcat ccgctgctgt
    13801 ttattatctt tttctataga tttcttaatt aatcaacgaa ttgcgtagtt tttgagcatg
    13861 ttaatgatta cgttgcagaa ctattacaat gtcatcagtt gtgaacaaag cacctggtcg
    13921 cgcatactag gggctataaa tttatcttta tcagaagcca tcacatgaaa gaagtcgaaa
    13981 aaaacgaaat caagcgtctc agcgatcgcc tggacgccat ccgccaccag caggccgatc
    14041 tgtcgctggt tgaagccgca gacaaatatg ccgagctgga aaaagagaaa gccacgctgg
    14101 aagcagaaat tgctcgcctg cgtgaggttc atagccaaaa actgagtaaa gaagcacaga
    14161 agctgatgaa gatgccgttc cagcgcgcaa ttaccaaaaa agagcaggct gatatgggca
    14221 agctgaagaa aagtgttcgc ggactggtgg ttgtgcaccc aatgaccgcg ctggggcgcg
    14281 aaatgggcct ggaagagatg actggatttt ctaagactac gttttaagcc cctcagacga
    14341 ctggttcgat cgtatcaacc cgcatctggc ttgccttagc tggtcaatgc ggggactctc
    14401 ctgtctactc gcctaattac caaacaa
//
LOCUS       DAZPCS010000093        14319 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZPCS010000093 DAZPCS010000000
VERSION     DAZPCS010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14319
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.093"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            1..3052
                     /locus_tag="ACHUQ7_002686"
                     /pseudo
     CDS             1..3052
                     /locus_tag="ACHUQ7_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            3062..6708
                     /locus_tag="ACHUQ7_002687"
                     /pseudo
     CDS             3062..6708
                     /locus_tag="ACHUQ7_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            6769..7176
                     /locus_tag="ACHUQ7_002688"
     CDS             6769..7176
                     /locus_tag="ACHUQ7_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1136038.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDVDPLINDALIYIRDEFIDEQKLRGESPEPVIVIFNCKLQNIFNQ
                     QIELYTLDNSSRSTCNLKYDEYSFIKGGNGIPP"
     gene            7718..8806
                     /locus_tag="ACHUQ7_002689"
     CDS             7718..8806
                     /locus_tag="ACHUQ7_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFR1136039.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATSISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            8817..11096
                     /locus_tag="ACHUQ7_002690"
     CDS             8817..11096
                     /locus_tag="ACHUQ7_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HFR1136040.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQADRRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDSPGDALNRQREAFMARWIGWAMQQNNGDVLVVCGGWHAPALAKMWRECPQVIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLLEAARHKLEADMLDAGGIGSITTCLSQAASAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGVSLSAAVALLERHSQAIHVLALDRGATLGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DADFTNALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            11089..12225
                     /locus_tag="ACHUQ7_002691"
     CDS             11089..12225
                     /locus_tag="ACHUQ7_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFR1136041.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSSGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIEFPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAMEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            12222..14225
                     /locus_tag="ACHUQ7_002692"
     CDS             12222..14225
                     /locus_tag="ACHUQ7_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1136042.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     SALIATFSDGVRTQLANGQALKEAQCTYGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKSITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARSDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQEDSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAIERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVVVSLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAINQAELLANDLTA
                     VGFYRLAHVLAQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
BASE COUNT         3547 a         3755 c         3654 g         3363 t
ORIGIN      
        1 tgggattatg cgaataatgt cattattatt ctgccgtctg gcattgcacc tgactacttg
       61 gagcaatatt ccagatttga attacgccta cgtaaacatt tatcactggc ggaagagtct
      121 ctctggcaaa aatgtgcaca aaaacttatt gccgcaattc cacatattcc agaatggcgg
      181 caaccattaa ttgctttgtt attacccgaa aaaccagaaa ttgcacatga aattgcccag
      241 catctactgg ggcaaaaaaa attaccctcg cttgagtggt taaaaatagt ggcgactgat
      301 gagcacattc ttgcctcatt agaaaaatat cacgagccat atgccatttt tgatgattac
      361 tattgtggtg cgatatggtc agccaccgta ttacaggagc aaggtgttgc agccctgccc
      421 cgatttgctc cctatgccgc aagtgactac tgcgccgatg tgttgcgtca tatcaatcat
      481 ccgttcgcat taacactgct tatacgtgta gccgggcata ctaaacgctg tcacgatcga
      541 atgacgaaag cctgcgctgc gttcccacac gcagcactgg cagcactggc ggaacttcta
      601 gtgcaaaaag aagagaatag ttggcgcatt atgctaatga cgatgtttat ctcgcaacca
      661 gcactggcag aacaggtcat tccctggctt tcgacacccg cagttgcagt gctgaaatca
      721 tgccagcacc aactgacaca gccctcaaac catgccagcg ccgatctact gccagccata
      781 gtagtctccc caccatggat ttcgaaaaag aaaaaatcgc cgattccggt gctggattta
      841 gcgccattag gcatcgagcc aatctgttat ctgacagaag aaatcagtaa tcaacttttg
      901 gcgaaatata tctggtattc aaaacacatc acggttagcc atgaagaaag tactaccaac
      961 ctgttggcaa ggatgggttt tcaacgacgg atcgctggta catatattaa agctcccgaa
     1021 gcggtagttg aggcatggct aaatgaagat tattcaacct tactaagtga atttaaggtg
     1081 tttcattcac ctaccgggca ttattggcag ttggggattt tgacaacatt gccgctggag
     1141 gctggagaaa gcagtaaaag catggaatgc ccttactcta tctccacata ccgataccga
     1201 atacgccatg ttacattttg gactcaaagg tttacctggt ttagttaact caattacacg
     1261 ctatccacag gaagccttgc ccatcacgaa ttacttcgca gcgagtgagc tggctcctgc
     1321 cgtcgcccgt gccttcaaca aactcaaaac gctacgcgaa aacgcccgca cctggctgtt
     1381 gaaatacccg gaacatgccc ttaccggcct gctgcctgcg gcgctcggca aagccggtga
     1441 agcacaggat aacgcccgcg ctgccttgcg tatgcttacc gaaaacggtc atcagccatt
     1501 actgcaagaa atcgcccggc gttataacca gtcggaagta acagacgcgg tgaacgctct
     1561 gcttgcgctc gatcccttag ataatcaccc gacaaaaatc cccactcttc cgacctttta
     1621 tcagccatcg atctggacgc gcccggtatt aaaagcaaac gcccaatcac tgccagataa
     1681 cgccctcctc cacctcggcg aaatgctccg cttccctcag gaagaggctc tgtatccggg
     1741 attattgcag gtgaaagacg cctgtaccgc tgactctctg gctgaatttg cctgggatct
     1801 gtttaccgcc tggcagaccg ctggcgcgcc gtcgaaagag agttgggcgt tcactgcgtt
     1861 aggcgttctc ggtaacgatg acaccgcccg caaactgacg ccattaatac gcgcctggcc
     1921 tggtgaatcc cagcataaac gcgccaccgt tgggttggat attctcgccg ctatcggtag
     1981 tgatatcgcc ctgatgcagc ttaacggcat tgctcagaaa ctgaaattca aagcattaca
     2041 ggagcgggca aaagaaaaga ttgccgacat tgccgagagc cgcgaactca cggtagcgga
     2101 gcttgaagat cggttagcac cggatctcgg tctggatgat aacggttcgt tgctgctgga
     2161 ttttggccca cgccagttca ccgtcagctt tgatgaaacc ttaaaaccgt ttgtgcgtga
     2221 tgtttccggc agccgcctga aagacctgcc aaaaccaaac aaaagcgatt atgagtcaca
     2281 ggcgaacgat gctgttaacc gctacaaatt gctgaaaaaa gatgcgcgta ccgtcgcggc
     2341 ccagcaggta gcaaggcttg aatccgccat gtgcctgcgt cgccgctggt cgccggaaaa
     2401 cttccaactc ttcctggttg tgcatccgct ggttcgccac ttaacccgcc gtctgatttg
     2461 gggcgtttat agcgccgaca accagctact ggcctgtttc cgggtagcgg aagataacag
     2521 ctacagcacc gctgacgatg atcttttcac cctgccggaa ggcgatatct ctatcggcat
     2581 tcctcacgtt ctggaaatat caccgacgga tgctgtcgcc tttggtcagc tttttgccga
     2641 ctacgaactg ctaccaccgt tccgtcagct tgaccgtaac agttacgccc tgacagaagc
     2701 cgagcgcaac gccagcgaac tgacccgctg ggcaggcaga aaatgcccga gcggccgggt
     2761 catggggctg gcaaataaag gctggataaa gggcgaacca caggatggag gctggatgat
     2821 caaacctctc ggtcgctggt cgttaatcat ggaaatcgac gaaggttttg cggtgggtat
     2881 atcaccagcc gaacttagcg ctgagcagct cttaagcaag ctgtggctat gggaaggcaa
     2941 agcggaaagc tatggctggg ggagtaattc aacacaggaa gcgcagttct ccgtactcga
     3001 tgccatcacc gccagcgagc taattaacga tattgaagcc ctgtttgaat aaggaaagag
     3061 catggacaag gaattaccgt ggctggcgga taacgcccaa ctggaactga aatataaaaa
     3121 aggcaaaaca ccactcagtc atcgcaattg gccgggcgaa ccagtgcctg ttatcactga
     3181 aagcatcatt cagacactgg gtgatgaatt gctacaaaaa gctgagaaga aaaaaaacat
     3241 tgtctggcgt tatgaaaatt tttcaccgga gtggcagtcc gccattacgc aggccatcaa
     3301 cttgatcggc gaacacaaac cctcagtccc ggcccgcaca atggcggcac tagcctttat
     3361 cgcgcaaaat gacaaccaac agttgctcga cgaaatcgtc caacaagagg ggctggaata
     3421 tgcgactgag gtggtgattg cacgccagtt tattgcacgg tgttatgaga gtgatcctct
     3481 ggtagtcaca ttgcagtatc agaacgagga ctatggctat ggttatcgct cagaaaccta
     3541 taacgaattc gatctccgac tgcgtaaaca cctctctctg gcagaggaaa gctgctggca
     3601 gcgttgcgcc gacaaactca ttgccgcact gccaggaata accaaagttc gccgcccttt
     3661 tattgcgctg atcctccccg aaaaaccaga aatcgccaat gagttggtaa gccttgaatg
     3721 cccgcggact cattttcatt ctaaggagtg gttaaaagtt gttgctactg accccaaagc
     3781 ggtgagaaaa ctcgaacgct actggagcca ggatatattt agcgatcgag aagccagcta
     3841 catgtcgcat gaaaaccact tcggctacgc ggcctgcgct gcccttttgc gcgaacaagg
     3901 actggcagtc gttccgcgcc tcgcgatata tgcccataaa gaagattgcg gcagtctgct
     3961 ggtacaaatt aaccatccgc aagtcatccg caccttgctg ttagttgctg ataaaaacaa
     4021 acccagcctg caacgtgtgg ctaaatacag taaaaacttc ccccatgcga cactcgccgc
     4081 actggcagaa ctgctggcgt taaaagaacc accagcccgc ccaggttatc caatcatcga
     4141 agacaaaaag ctgcctgcac agcaaaaagc tcgagatgaa tactggcgta cgctgttaca
     4201 aacgctgatg gcatcgcagc cacaactggc agcagaagtg atgccgtggt taagtactca
     4261 agcccgggca gtgctgaata gttatttatc ggcaccgccc aaaccagtta ttgatagcac
     4321 cgataacagc caaatgcctg aaatcctcgt ttcaccacca tggcgtggga gaaagaaaaa
     4381 gacttccata cgtcactttt cgctgcctga tcttacgctt gcaccgcaaa cgtacaggtc
     4441 gccgaataaa cactggacgc cggaacagca aactgcaatt gaccatttta gcgccatgtc
     4501 ttttgacgaa cgtcttgcta cgagaggagc ggatatcttc ttgcgggaat taggttttga
     4561 cgttcatagc ctggaattta ataagcgtct tcttaatgga cagttggatg cctttgatgc
     4621 tcagcaactg caagaataca catcaatagg actattgcgt acttatcacg cgcatcaggc
     4681 caaatctaaa cagtaccggg aagacgccac caatgcatta ctggcacaag atagcattgc
     4741 gttgatggga gggtggcgaa cttttcagca cggattttac tatggtaatg aacagcgttg
     4801 ggggatttgg aatctgtacc ttatcgcgca gatgccgcgc gaaatggcgg tatcgtgctg
     4861 gcaacaaatt gtcgaggctg attttcatta caccggggtg gaatatttgt taagtgtgct
     4921 gggtaccgat gctttgcctg gattaaacac cgcctttgcc cgacatccaa aagagatatt
     4981 tccgctgctg attcatttcg gcgcaacaga actggccctg cccattgccc gcgtctggca
     5041 cctttttgcg ggccagcgta atctggctcg ccagtggatt ttacaatggc cggaacatac
     5101 ggctactgca cttatccctc ttgtcttcac caaatctagc gataaaagcg aagctgcatt
     5161 acttgccctg cgtttacttt acgaacatgg gcatggcgaa tggctacaaa ctgtagccaa
     5221 tcgttggcag cgtacagatc tatggcctgc cctggaacaa ttacttaaac agagtccaat
     5281 agagatttac ccggcacgca ttccaaaagc ccctgatttc tggcatccgc aaatgtggtc
     5341 caggccgcgc cttatcacta ataatcaacc tgttaccgat gacgctctgg aaattatcgg
     5401 cgaaatgctg cgctttaccc aggggggacg tttttatagc gggctggaac aactgaaaac
     5461 gttctgccag ccacaaacgc tggcagcttt tgcctgggat ctcttcactg cgtggcaaca
     5521 agctggtgcc cccgcaaaag acaactgggc atttctggcg ttaagtctct ttggtgacga
     5581 aagcacggca cgggatctaa cgacacatat cctcgcctgg ccacaagaag gcaaatctgc
     5641 ccgtgcggtc agtggcatga acatccttac cctgatgaat aatgatatgg cgctgatgca
     5701 gctgcatcat atatcgcaac gggcgaaatc ccgcccctta cgtgataacg cggcggaatt
     5761 tcttcaggta gtcgcagaaa atcgcgggct aagccaggaa gagttagcgg acagattagt
     5821 cccaacactg ggccttaatg atccgcaggc gttgagtttt gattttggtc cccggcagtt
     5881 taccgttcgc ttcgatgaaa acctcaaccc ggttatcttt gatcagcaaa acgttcgcca
     5941 gaaaagtgtt ccccggttgc gcgccgatga cgatcaactg aaagcgcccg aggcactggc
     6001 ccgactaaaa gggttaaaaa aagatgctac tcaggtgagc aaaaacctgc tcccgcgtct
     6061 tgaagctgcc ctacgtacca cccgacgctg gtcgctggca gattttcatt ctctgtttgt
     6121 taatcatccc tttacccgtc tggttaccca gcgattaata tggggggtta tccggcaaat
     6181 gaaccgcgtc gtttactcaa cgcctttcgt gtggccgcag agggggagtt ctgcaatgcg
     6241 caagatgagc caattgacct gcctgcggac gctctgattg gcattgccca cccgttagaa
     6301 atgacagcag aaatgcgcag tgaatttgca cagctttttg ccgattacga aattatgccg
     6361 ccttttcgcc agttgtcgcg ccgcacggtg ctgctcacac ctgacgagtc aaccagtaac
     6421 agcctgactc gctgggaagg taaatccgct accgttgggc aacttatggg aatgcgatac
     6481 aaaggctggg agtcaggtta tgaggacgca tttgtctatg acctgggtga gtaccggctg
     6541 gtccttaagt tttcacccgg ttttaaccac tacaatgttg atagcaaagc gctaatgagc
     6601 ttccgttctc ttcgagtgta ccgtgacaat aaatccgtca cttttgccga acttgatgtg
     6661 tttgatttga gtgaggcgtt aagcgcacct gacgtcattt tccattaaaa caaagcgggc
     6721 agtgatgcga ctgcccgtta tctacacgac ttaccagcgg ggaaagcgat gatcgtgcaa
     6781 aaagagctgg ttgctattta cgattatgag gtccctgtac ctgaagatcc gttttccttc
     6841 agacttgaga tccataaatg ctctgaatta tttacaggtt ccgtctaccg actggaacgg
     6901 ttccggctac gtccaacatt tcatcaacgt gatcgagagg atgttgaccc gctaataaat
     6961 gatgcgttga tttatataag agatgagttt attgatgagc aaaaattacg aggcgaatca
     7021 cctgaacctg taatagtaat ttttaattgt aaactacaga atatattcaa ccaacaaata
     7081 gaattatata ctctagataa ttcaagtcgc agcacatgca acttgaagta tgacgagtat
     7141 agctttatta aaggggggaa cggtattccc ccttagatgc gtttcaattt gcccgttact
     7201 tcaacaaatc aatcaaccgt aacagagcca ctaatagctc cagtacagca atgagcacgg
     7261 ttaacttttg tgtcagcgtg tccatccgct ctccttatta aggagcacaa caccagcacc
     7321 ttacccttac actgcctgtc gatactggtt tacgtttgtt gagtcgccat gttgcactcc
     7381 ggatcagagc actacctgcg gcaccacccg tccggtagcc aggacttgaa ataggttcga
     7441 tccggcgcaa cttcccaaca gtcaggatta taaatctcat tatcttaatt gcaacactgt
     7501 ccagaggtgg ttataattcg ttcgttggaa cacgatgttg cacaggcagt ggtgttaggc
     7561 ctgaaaatag taaataaaag aacctgcctc accagcagtt ttttttattt actgtgatct
     7621 gctttccaga tatttttcgc tcaaacaact aatgcgccaa acatttattg cgcgtaaaat
     7681 atcgtttatt tcattaatac atttcaggga tgaatatatg tcaccgcaga ataatcatct
     7741 gcagcgtccg cctgctgctg tgttatacgc cgatgaactg gcaaaattaa aacaaaatga
     7801 taacgcacct tgcccgcccg gttggcagtt aagtttgcct gcggcccgtg cttttatcct
     7861 tggcgatagc gcgcaaaata ttagccgtaa agtggtgatt agtccctccg ctgtcgaacg
     7921 tatgttagtc actcttgcca ccggacgcgg tttgatgttg gtgggggaac ctggcaccgc
     7981 aaaatctctc ctgtctgaat tactggcaac ctctattagc ggcgatgccg ggttaaccat
     8041 ccagggcggc gcatctacta ccgaagatca aatcaagtac ggctggaact acgccctgct
     8101 tatcaatcat ggtccatcaa cggaagcgct ggtccctgcc ccgctttacc agggaatgcg
     8161 cgacggcaaa atcgtccgtt ttgaagagat aacgcgtaca ccgctggaag tgcaagactg
     8221 tctgttaggt atgctttctg acagggtgat gacggtgccg gaactcactg gtgaagccag
     8281 tcagctctat gcgcgagaag gctttaatat tattgccact gccaataccc gcgatcgcgg
     8341 cgtcaatgaa atgagtgctg cgctaaagcg tcgcttcgat tttgaaactg tctttccgat
     8401 tatggatttc gcccaggagc tggagcttgt cgccagtgct tcggcgcgtt tgctggccca
     8461 tagcggtatt ccgcataaag tgcctgacgc ggtactggag ttactggtcc gcaccttccg
     8521 cgatctgcgt gccaacggcg aaaagaaaac gtcaatggat acactaacag cgattatgtc
     8581 caccgctgaa gccgtgaacg tggcccacgc cgtaggcgtc cgcgcctggt ttttagcgaa
     8641 tcgcgcaggt gagccagccg acctggtgga ctgcattgcc ggaaccatcg tcaaagataa
     8701 cgaagaagat cgcgctcgtc tgcgccgtta cttcgaacag cgtgtcgcca cacataaaga
     8761 agctcactgg caggcttatt atcaagcccg ccaccgcctg ccgtgaggaa agatgcatga
     8821 gcgagccgtt aattgtcggc atccggcatc atagtccggc ctgcgcccgg ctggtgaaat
     8881 cgttaatcga aagccagcgg ccacgatacg tgttgattga aggccctgct gattttaatg
     8941 accgggtaga cgaactgttt ttagcccacc agcttccggt agctatttac agttattgcc
     9001 agtatcagga cggtgcagcc cccgggcgtg gtgcctggac gccatttgct gaattttcgc
     9061 cggagtggca ggcgctacaa gccgatcgtc gtattcaggc acaaacttac ttcatcgatt
     9121 tgccttgctg ggcgcagagt gaagaagagg acgattcgcc tgatacgcaa gaggaaagcc
     9181 agaccttgct gctgcgtgcc acccgcatgg ataacagcga caccctgtgg gatcacttgt
     9241 tcgaagatga aagccagcaa actgcattac cctctgcact ggcgcactat tttgcccaac
     9301 tgcggggcga ctcccccggc gatgctctaa atcgtcagcg cgaagccttt atggcccgct
     9361 ggattggatg ggcgatgcag caaaataatg gcgacgtgtt agttgtctgc ggtggctggc
     9421 acgctccggc actggcaaaa atgtggcgcg aatgtccgca ggtaattaac acgccagaat
     9481 tgccgtcgct ggcagatgcc gttacaggtt gttatctcac cccctacagt gaaaagcgcc
     9541 ttgatgtgct ggcaggatac ctttcaggaa tgcctgcccc ggtctggcaa aactggtgct
     9601 ggcagtgggg cttacagcag gcgggagaac aactgctaaa aacggttctc acccgtttgc
     9661 gccagcacaa gttgcctgct tcgaccgcgg atatggctgc cgctcatctg catgcgatgg
     9721 cactggcaca gttgcgcggt catacactac cgttacgcac tgactggctg gatgccatag
     9781 caggatcgct gattaaagaa gccctgaatg cgccgttgcc gtggagctat cgcggcgtta
     9841 ttcatcccga taccgatccg attctgctaa cgttgataga cacattagcg ggtgacggat
     9901 tcggtaaact tgccccttct acgccacaac cgcctctgcc aaaagatgtc acttgcgaac
     9961 tggaacgtac cgcaatctct cttccggcgg agcttacctt aaatcgcttt aaccccaatg
    10021 gactagcgca aagtcaggtg ttacatcggc tggcaatact ggagatccca gggattgtac
    10081 gccagcaggg aagcacactg acacttgcag gcaacggtga agaacgctgg aaattaaccc
    10141 gcccgcttag ccagcatgcg gcattgattg aggccgcctg ctttggtgcc acactcctgg
    10201 aagccgcacg ccataaatta gaagccgata tgctggacgc gggtggaatc ggcagcatta
    10261 ccacatgtct tagccaagcg gcgtcagcgg gtctggcgtc cttcagtcaa caattactgg
    10321 agcaactcac attattaatc gcccaggaaa atcaatttgc cgaaatgggc caggcgctgg
    10381 aagtgctata tgccttatgg cggctggatg aaattagcgg tatgcaaggc gcgcagatat
    10441 tacaaacgac gttatgcgcg gctatcgatc gcacgctgtg gctgtgtgaa tccaacggca
    10501 gaccggatga aaaggagttt cacgctcact tgcatagctg gcaagcgttg tgtcatatcc
    10561 tgcgcgatct acatagcggt gtgcaactcc ccggcgtttc gctttctgcg gcggtagcct
    10621 tgctggagcg acacagtcag gcgattcatg tcctggcact ggatcgcggc gcgactcttg
    10681 gtgcgctgat gcgtctggaa catcccaacg ctagtgccga agcggcgctg acgatgctgg
    10741 cgcagttatc cccggcacaa tccggcgagg cgctgcacgg tttgctggca ttagcccgtc
    10801 atcaactggc ctgtcagccg gcatttatcg ccggtttcag cagtcattta aatcaactaa
    10861 gtgatgccga ttttaccaat gccctgcccg atttacgcgc ggcaatggcc tggctaccgc
    10921 cacgagaacg cgggacgctg gcgcatcagg tgcttgagca ttatcaactg gcgcaacttc
    10981 ccgtttcggc actgcaaatg ccgttgcatt gtccaccgca agccattgca catcatcaac
    11041 aactcgaaca gcaggcactg gcatcgctgc aacactgggg agttttccat gtctgaactg
    11101 aacgatcttc tgaccacccg tgagctacaa cgctggcgat taattcttgg cgaagcggca
    11161 gaaacgacgc tttgtgggct ggatgacaac gcccggcaga tagatcacgc gctggagtgg
    11221 ctgtatgggc gcgatcctga acggctccag cgtggtgaac gctctagtgg attaggtggc
    11281 tcaaatctca ccactcctga gtggatcaac agtattcaca cgctgtttcc gcaacaggtg
    11341 attgagcggc tggaaagcga tgccgtactg cgctacggca ttgaagatgt ggtgacaaat
    11401 ctcgacgtgc tggaacgtat gcagccttcc gaaagcctgc tacgcgccgt tttgcacacc
    11461 aaacatctga tgaaccccga agtactggct gccgcccgca ggattgtgcg ccaggttgtg
    11521 gaagaaatta tggcgcgact ggcaaaggaa gttcgtcagg ctttttctgg tgtccgcgat
    11581 cgtcgtcgcc gctcatttat tccactggcg cgaaactttg atttcaaaag tactctgcgc
    11641 gccaacctgc aacactggca cccgcaacac ggcaagttgt atatcgaatt cccccgcttt
    11701 aacagccgca ttaaacgcca aagcgagcaa tggcaactgg tcttactggt tgatcaaagc
    11761 ggatcgatgg tcgattcggt gatccactct gctgtgatgg cggcctgttt atggcagtta
    11821 cccggcattc gtacccatct ggtggcgttt gacacaagcg tcgttgatct cacggcagac
    11881 gttgccgatc cggtagagtt attaatgaaa gtgcaattag gcggcggaac caatatcgcc
    11941 agtgccatgg agtatggtcg gcaacttatt gaacaacctg caaaaagcgt cattatcctc
    12001 gtgagcgatt tttatgaagg gggttcatca tcattgctga cgcaccaagt gaaaaagtgt
    12061 gtccagagcg gcatcaaagt gctgggactg gcagcgctcg atagcacagc aacaccttgc
    12121 tatgaccgcg atacggccca ggcgctggtt aatgtcggcg cacaaatagc cgccatgaca
    12181 ccgggcgagc tggcatcatg gcttgcggag aatcttcagt catgaattca ctacgtccgg
    12241 aattattaga actgacaccg caggcactga cggcgttaag caatgccggt tttgttaagc
    12301 gcagccttaa ggaactggaa aatggcaacg tcccggaaat cagccatgag aacagcgctt
    12361 taatcgccac cttcagtgac ggtgtccgta cccagctggc gaacggccag gcactgaaag
    12421 aggctcagtg cacctatggg gccagcggca tgtgccgtca tcgcgtgatg ctggtgttaa
    12481 gttatcaacg actttgtgct accgcccagc ctacgggaaa agaagaggaa tgggatccgg
    12541 caatctggct ggaagaactg gctacccttc ccgatgccac ccgcaaacgc gcacaggcgc
    12601 tggtcgctaa aagcatcacc attgagttgt tctgtgcgcc gggtgaaatt ccctctgccc
    12661 gcttaccgat gagcgatgtg cgtttttatt cccgcagcag tattcgtttc gcccgttgtg
    12721 attgtattga aggcacactt tgcgaacatg tcgtactggc agtacaggcc tttgtccagg
    12781 caaaagcgca gcaagcggaa ttgactcatt taatctggca gatgcgcagc gaacacgtca
    12841 catcatctga cgatccgttt gccagcgaag aaggcaaaac gtgtcgtcaa tatgttcaac
    12901 aactaagcca ggcattatgg ctgagcggca tcagccagcc gcttattcac tacgaggccg
    12961 ctttcagccg cgcgcagcaa gcagcggaac gctgcagctg gcgatgggtg agtgaatcgc
    13021 tacggcaact gcgcgccagc gttgatgcct tccacgcccg cgccagccac tatcatgccg
    13081 gagaatgctt acgtcagctt gcggcattaa acagtcgatt aaattgcgca caagagatgg
    13141 cccggagcga cagtgttggt gaagttcctc ctgtgccgtg gcgcacggtc gttggctctg
    13201 gcattgccgg agaagcaaag cttgatcatc tgcggctggt gtctttaggt atgcgttgct
    13261 ggcaggatat tgagcattat ggtttacgca tctggtttac cgatcccgac accggcagta
    13321 ttttgcacct ttcgcgcagt tggccgcgaa gtgaacagga agactcaccg gcagctacgc
    13381 gtcggctgtt tagttttcag gctggcgcac tggcgggtgg gcaaattgtt tcacaagcag
    13441 caaaacgcag tgccgatggc gagctgctgt tagctacccg caaccgctta agcagcgttg
    13501 taccgctgtc gcctgatgcc tggcaaatgt tgagcgcgcc gttacgccag ccgggcattg
    13561 tggctttgcg ggaatattta cgccagcgtc cccccgcctg catacggcct cttaatcagg
    13621 tcgataactt atttattctg ccggtcgctg agtgtatttc gctcggttgg gatagcagcc
    13681 gccagacgct ggatgcgcag gtaatcagcg gcgaaggtga agataatctg ctgacgttat
    13741 cactgccagc ctcagccagc gcgccttatg ccattgaacg catggcggcg cttttgcaac
    13801 aaacagacga ccccgtgtgt ctggtttctg gctttgtcag ttttgttgat gggcaattga
    13861 cactggaacc acaggtaatg atgacaaaga ctcgtgcctg ggcgctggac gcagaaactg
    13921 cgcctgtggt ggtatcacta ccttctgcca gcgttctgcc tgtgccgtct actgctcatc
    13981 agttgctgat gcgctgccag gcgttactta ttcaactgct ccataacggc tggcgctatc
    14041 aggaacagag cgctattaat caggcagagt tgctggcgaa tgacctcacc gcggtgggtt
    14101 tttatcggct ggcacatgtg ttggcacaat ttcgtaatac agaaagcgag gcacgggtag
    14161 aagcaatgaa taacggtgtt ttgctttgcg aacaattatt ccccatgctt cagcaacaag
    14221 gatgaaatag tgctttttac taagagttct actccagttc cggactgctc acgccacggt
    14281 attaggcata tcctatatag cccctggtga gagtcacca
//
LOCUS       DAZPCS010000094        14233 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZPCS010000094 DAZPCS010000000
VERSION     DAZPCS010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14233
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.094"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            174..1232
                     /gene="citC"
                     /locus_tag="ACHUQ7_002693"
     CDS             174..1232
                     /gene="citC"
                     /locus_tag="ACHUQ7_002693"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFR1136043.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1247..1543
                     /gene="citD"
                     /locus_tag="ACHUQ7_002694"
     CDS             1247..1543
                     /gene="citD"
                     /locus_tag="ACHUQ7_002694"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFR1136044.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILHARLEALLARASGIPALPWEDCQ"
     gene            1540..2448
                     /gene="citE"
                     /locus_tag="ACHUQ7_002695"
     CDS             1540..2448
                     /gene="citE"
                     /locus_tag="ACHUQ7_002695"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFR1136045.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHAHRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2459..3991
                     /gene="citF"
                     /locus_tag="ACHUQ7_002696"
     CDS             2459..3991
                     /gene="citF"
                     /locus_tag="ACHUQ7_002696"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFR1136046.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVNRVGDAAKIGAGTTRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDRQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            3995..4545
                     /gene="citX"
                     /locus_tag="ACHUQ7_002697"
                     /pseudo
     CDS             3995..4545
                     /gene="citX"
                     /locus_tag="ACHUQ7_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
     gene            4520..5398
                     /gene="citG"
                     /locus_tag="ACHUQ7_002698"
     CDS             4520..5398
                     /gene="citG"
                     /locus_tag="ACHUQ7_002698"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFR1136047.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPTDLDYLRQFDRECIERNLSP
                     GGSADLLILTCFLAQI"
     gene            5449..6911
                     /gene="citT"
                     /locus_tag="ACHUQ7_002699"
                     /pseudo
     CDS             5449..6911
                     /gene="citT"
                     /locus_tag="ACHUQ7_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
     gene            7025..7831
                     /gene="rna"
                     /locus_tag="ACHUQ7_002700"
     CDS             7025..7831
                     /gene="rna"
                     /locus_tag="ACHUQ7_002700"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFR1136048.1"
                     /translation="MKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKTDFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8060..8470
                     /gene="rnk"
                     /locus_tag="ACHUQ7_002701"
     CDS             8060..8470
                     /gene="rnk"
                     /locus_tag="ACHUQ7_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFR1136049.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            8608..8697
                     /gene="yldA"
                     /locus_tag="ACHUQ7_002702"
     CDS             8608..8697
                     /gene="yldA"
                     /locus_tag="ACHUQ7_002702"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFR1136050.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(8701..9910)
                     /locus_tag="ACHUQ7_002703"
                     /pseudo
     CDS             complement(8701..9910)
                     /locus_tag="ACHUQ7_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
     gene            10131..10559
                     /gene="uspG"
                     /locus_tag="ACHUQ7_002704"
     CDS             10131..10559
                     /gene="uspG"
                     /locus_tag="ACHUQ7_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFR1136051.1"
                     /translation="MYKAIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10680..12245)
                     /gene="ahpF"
                     /locus_tag="ACHUQ7_002705"
     CDS             complement(10680..12245)
                     /gene="ahpF"
                     /locus_tag="ACHUQ7_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFR1136052.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAENRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12490..13053)
                     /gene="ahpC"
                     /locus_tag="ACHUQ7_002706"
     CDS             complement(12490..13053)
                     /gene="ahpC"
                     /locus_tag="ACHUQ7_002706"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFR1136053.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13425..14171
                     /gene="dsbG"
                     /locus_tag="ACHUQ7_002707"
     CDS             13425..14171
                     /gene="dsbG"
                     /locus_tag="ACHUQ7_002707"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFR1136054.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFSDPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
BASE COUNT         3401 a         3760 c         3573 g         3499 t
ORIGIN      
        1 aaatcaaaat ttcacgccga aatactcctt aggatgtata gcaaaaggag aaaaagatat
       61 acctcgatca cccccctttc tcccaagtga aaataaaagg ttatcagttt gcaacattga
      121 acaacattcg ttgcaaatcg ataacaacat gcaccttcag gatactattt attatgttcg
      181 gcaatgatat tttcacccgc gtaaaacgtt cagaaaataa aaaaatggcg gaaatcgccc
      241 aattcctgca tgaaaatgat ttgagcgttg acaccacagt cgaagtattt attaccgtaa
      301 cccgcgatga aaagcttatc gcgtgcggtg gaattgccgg aaatattatt aaatgcgttg
      361 ctatcagtga atccgttcgc ggtgaaggac tggcgctgac attagccact gaactgataa
      421 acctcgccta tgagcggcac agcacgcatc tgtttattta taccaaaacc gaatacgagg
      481 cgctgttccg ccagtgcggt ttttccacgc tgacctgcgt acccggcgtg atggtgctga
      541 tggaaaacag cgccacgcga ctgaaacgct atgccgaatc gctgaaaaaa tttcgtcatc
      601 cagggaacaa gattggctgc attgtgatga acgccaatcc ctttacgaat ggtcaccgtt
      661 atctgattca acaagctgcg gcacagtgcg actggttgca tctgttttta gtcaaagaag
      721 attcttcacg cttcccctat gaagaccggc tggatctggt gttaaaaggc accgccgata
      781 ttccacgcct gactgtgcat cgcggctcag aatacatcat ctcccgcgct acgttccctt
      841 gctacttcat taaagaacag agcgtcatta accattgtta caccgaaatt gatctgaaga
      901 ttttccgtca gtacctcgct cccgcgcttg gtgtaactca ccgctttgtc ggtactgaac
      961 ccttttgtcg cgttaccgcc cagtacaacc aggatatgcg ctactggctg gaaacgccga
     1021 ctatctccgc agcgcccatc gaactggttg aaattgagcg gctgcgttac caggagatgc
     1081 cgatatccgc ttcccgggta cgtcaactgc tggcgaaaaa cgatctcacg gctatcgcgc
     1141 cgctggtccc tgcagtcacg ctgcattatt tgcagaacct gcttgagcac tcccgccagg
     1201 acgcggcagc tcgtcaaaag acccccgcat gagaaacagg tgaaaaatga aaataaacca
     1261 gcccgccgtt gcaggcaccc ttgagtctgg ggatgtgatg atacgcatcg ccccactcga
     1321 tacgcaggat atcgacctgc aaatcaatag cagcgttgag aaacagtttg gcgatgcaat
     1381 tcgcaccacc attctggacg ttctcgcccg ctacaacgtg cgcggcgtac agctgaatgt
     1441 cgatgacaaa ggcgcactgg actgcatttt acatgcacga ctggaagccc tgctggcacg
     1501 cgccagcggt atcccggctc tgccatggga ggattgccaa tgatttccgc ttcgctgcaa
     1561 caacgtaaaa ctcgcacccg ccgcagcatg ttgtttgtgc ctggtgccaa tgccgcgatg
     1621 gtcagcaact ccttcatcta cccggctgat gccctgatgt ttgacctcga agactccgtg
     1681 gcattacgtg aaaaagacac cgcccgccgc atggtttatc acgcgctgca acatccgctg
     1741 tatcgcgata ttgaaaccat tgtgcgtgtc aacgcgctgg attccgaatg gggtgttaac
     1801 gacctggaag ccgtcgttcg cggtggtgcg gacgttgtgc gtctgccgaa aaccgatacc
     1861 gctcaggatg ttctggatat tgaaaaagag atcctgcgta tagaaaaagc ctgtggtcgt
     1921 gaacccggca gcaccggcct gctggcggcg attgaatctc cgctgggcat tacccgcgca
     1981 gtggaaatcg ctcacgcttc cgagcgtttg atcggtatcg ccctcggtgc agaagactat
     2041 gtgcgcaacc tgcgtacaga acgctccccg gaaggaactg aactgctgtt cgcacgctgt
     2101 tccattttgc aggccgcgcg ctctgcgggt attcaggcgt tcgataccgt ctattccgac
     2161 gctaacaacg aagccggatt cctgcaagaa gccgcccaca tcaaacagct gggctttgac
     2221 ggcaaatcgc tgatcaaccc gcgtcagatt gatctgctgc acaacctcta cgcaccgacc
     2281 cagaaagaag tggatcacgc ccaccgcgtc gtagaagccg ctgaagccgc cgctcgcgaa
     2341 ggcctcggcg tggtttccct gaacggcaag atggtggacg gtccggttat cgatcgcgcc
     2401 cgtctggtgc tctcccgtgc agaactttcc ggcatccgcg aagaataagg caatcaaaat
     2461 gacgcagaaa attgaacaat ctcaacgaca agaacgggta gcggcctgga atcgtcgcac
     2521 tgaatgcgat cttgccgctt tccagaactc accaaagcaa acctaccagg ctgaaaaagc
     2581 gcgcgatcgc aaactgtgcg ccaacctgga agaagcgatt cgtcgctcag gtttacagga
     2641 cggcatgacg gtttccttcc atcacgcttt ccgtggcggt gacctgaccg tcaatatggt
     2701 gatggacgtc atcgcgaaga tgggctttaa aaacctgacc ctggcgtcca gctccctgag
     2761 tgattgccat gcgccgctgg tagaacacat tcgccagggc gtggttaccc gcatttatac
     2821 ctctggccta cgcggtccac tggcggaaga gatctcccgt ggtctgctgg cggaaccggt
     2881 gcagatccac tctcacggcg gtcgtgtgca tctggtacag agcggcgaac tgaatatcga
     2941 cgtggctttc ctcggcgtcc cgtcctgtga tgaattcggt aatgccaacg gctacaccgg
     3001 taaagcctgc tgcggctccc tcggctatgc aatggttgat gccgacaacg caaaacaggt
     3061 cgtgatgctt accgaagaac tgctgcctta tccgcataat ccggcaagca ttgagcaaga
     3121 tcaggttgat ttgatcgtca aagttaatcg cgttggcgat gctgcaaaaa tcggcgctgg
     3181 tactacccgt atgaccacta acccgcgcga actgcttatt gcccgtagcg ctgcggatgt
     3241 gattgtcaac tctggctact tcaaagaagg tttctccatg caaaccggca ccggcggcgc
     3301 ttcgctggcg gtaacccgtt tcctggaaga caaaatgcgt agccgcgata ttcgcgccga
     3361 cttcgccctc ggcggtatta ccgcgacgat ggttgatctg cacgaaaaag gtctgatccg
     3421 caaactgctg gatgtgcaga gctttgacag ccatgctgcg caatcgctgg cccgtaaccc
     3481 caatcacatc gaaatcagcg ccaaccagta cgctaactgg ggttcgaaag gcgcatcggt
     3541 tgatcgtctc gacgtggtgg tactgagcgc gctggaaatt gacaggcagt tcaacgttaa
     3601 cgtgctgacc ggttctgacg gcgtactgcg tggcgcttcc ggtggtcact gcgataccgc
     3661 gattgcctct gcgctttcca tcatcgtcgc gccactggta cgcggtcgta ttccgactct
     3721 ggtggataac gtgctgacct gcatcacccc gggctccagt gtcgatattc tggtcacaga
     3781 ccacggtatc gcagttaacc cggcacgtcc ggaactagca gaacgtctgc aggaagcggg
     3841 cattaaagtg gtttccattg agtggctgcg cgaacgtgcg cgtctgctga ccggtgaacc
     3901 acagccgatt gaattcaccg accgcgtcgt tgccgttgtg cgttaccgcg atggctcggt
     3961 gatcgatgtt gtgcatcagg tgaaggaata agccatgcac ctgcttcctg aactcgccag
     4021 ccaccatgcg gtgtcaattc ccgagctgct cgtcagccgg gatgaaaggc aagcacggca
     4081 acacgcctgg ctcaagcacc atcctgttcc actggtctcc tttaccgtgg ttgcgcctgg
     4141 gccgattaaa gacagcgagg tcacacgccg aatttttaat catcgcgtga cagccttgcg
     4201 tgccttagcc acaaagcagg gctggcaaat tcaggagcag gctgcactgg tttccgccag
     4261 cgggccggag ggcatgttga gcattgccgc cccggctcgc gacctcaagc tcgccaccat
     4321 tgagcttgaa catagtcatc ctctcgggcg gttatgggat atcgatgtcc tgacgcccga
     4381 aggcgaaatt ctctcccgcc gcgactattc actgcgcctc gccgctgcct gttgtgcgag
     4441 caaagcgcag ccgtctgcgc gcgtggaaaa acccatcaac tgaccgattt actcaaccgc
     4501 atggaggcac tgctgaacga tgtcgatgcc tgcaacgtca actaaaacca caaagcttgc
     4561 gacgtcatta atcgatgagt acgccctgct gggctggcgc gccatgctga ctgaagtcaa
     4621 tctgtcaccg aaaccaggcc tcgtggatcg cattaactgc ggtgcgcaca aagatatggc
     4681 gctggaagat ttccaccgca gcgcgctggc gattcagggc tggttacccc gtttcattga
     4741 atttggtgcc tgtagtgcag aaatggcacc ggaagcggta ctccacggat tacgcccaat
     4801 tggtatggct tgcgaaggtg atatgttccg cgccactgcg ggcgtaaaca cgcataaagg
     4861 cagcattttt tctttagggc tgctatgtgc ggcaattggc cgtttgcttc agctcaacca
     4921 gccggttacg ccaacaaccg tttgttctac ggcggcaagt ttctgccgtg gcctgaccga
     4981 tcgcgaactg cgtaccaata attcacgact gacggcaggt caacggttgt accagcagct
     5041 tggccttacc ggcgcacgcg gtgaagccga agcaggttat ccactggtga tcaaccacgc
     5101 cttgccgcat tacctcactc tgctggatca ggggttagat cctgaactgg cattgctcga
     5161 taccttgctc ctactgatgg ctaccaacgg cgataccaac gttgcatcgc gcggtggcga
     5221 ggggggcctg cgctggctac agcgcgaggc gcaaacatta ttgcaaaaag ggggcattcg
     5281 aacccccacc gatctcgatt atctccggca gttcgacagg gagtgtatcg aacgaaatct
     5341 cagtccaggc ggcagtgccg acctgctgat ccttacctgt tttttagcac agatttaatt
     5401 atttaagcac ttgataaatt tggaaatatt aattttcgga gaacccgtat gtctttagca
     5461 aaagataata tatggaaact attggcccca ctggtggtga tgggtgtcat gtttcttatc
     5521 cctgtccccg acggtatgcc gccgcaggca tggcattact tcgctgtgtt tgtggcaatg
     5581 attgtcggca tgatcctcga gccaattccg gcaacagcga tcagttttat tgcggttact
     5641 atttgcgtta ttggcagtaa ttacctgctc tttgatgcca aagaattagc tgacccagcg
     5701 tttaatgcgc aaaaacaggc gctgaaatgg ggcctggctg gtttttccag caccacggta
     5761 tggctggtat ttggcgcatt tatttttgca ttagggtatg aagtttccgg gttaggtcgt
     5821 cgcattgccc ttttcctggt gaaattcatg gacaaacgca cgctgacgtt gggttatgcg
     5881 attgtcatta tcgacattct gctggcaccg tttacaccgt ccaacaccgc gcgtaccggg
     5941 ggtacggttt ttccggtcat taaaaacctg ccgccgttgt ttaaatcatt cccgaacgat
     6001 ccgtccgcgc gtcgtattgg cggctatttg atgtggatga tggtcattag taccagtctg
     6061 agttcgtcca tgtttgtcac cggtgcggac caaacgtgct gggtctggag ttcgtcagca
     6121 aaattgccgg tatccagatt agctggttgc agtggttcct ctgcttcctg ccggttgggg
     6181 ttatcttgct tatcattgcg ccgtggcttt cctacgtgct gtacaaaccg gaaatcacac
     6241 acagtgaaga agtggcaacc tgggcgggtg atgaactaaa aaccatgggt gcgctgacac
     6301 gcagagagtg gacgctgatt ggccttgtat tgctcagctt aggtttgtgg gtatttggca
     6361 gtgaagtcat taatgctact gcggttggtc tgctggcagt ttcgctaatg ctggctctgc
     6421 acgttgtgcc gtggaaagac attacccgct ataacagcgc atggaacacg ctggtcaacc
     6481 tggcaactct ggttgtgatg gctaacggcc tgactcgttc tggttttatt gactggttcg
     6541 ccggtaccat gagtacgcac ttggaaggat tctcaccaaa cgcaacggtg attgtactgg
     6601 ttctggtgtt ctactttgca cactacctgt ttgccagcct gtctgcgcac accgcaacca
     6661 tgctgccggt tattctggcc gtcggtaaag gtattccggg cgtaccaatg gaacaactgt
     6721 gtatcctgct ggtgctgtct atcggtatca tgggctgtct gacgccgtat gcaaccggtc
     6781 ctggggtgat tatttacggc tgtggctatg tgaaatcaaa agattactgg cgtcttggcg
     6841 caatcttcgg ggtgatttac atctctatgt tgctgttggt tggctggccg attctcgcca
     6901 tgtggaacta atacgttcgc caaacgctga ataaatcatc acgcccgcca ggtaagccca
     6961 cctggcgggc ttttttatga tttaatagat agtattcagt gtatgtatga gttccacacc
     7021 cattatgaaa gcattctgcc gtaacgccgc gttgctcgcg gtttctctgc ttcccttatc
     7081 ttctgccaac gccgtagcgt tgcaggcaaa acagtatggc gattttgatc gctatgtcct
     7141 ggccctctcc tggcaaaccg gattttgcca gagtcaacac gatcgaaatc gtaacgaacg
     7201 agatgaatgt cgcctgcaaa ccgaaacgac caacaaaact gattttctga ccgtacatgg
     7261 tctgtggcca ggattgccta aatcggttgc tgcccgtggt gttgatgaac gccgctggat
     7321 gcgcttcggt tgcgctactc gcccaatccc gaatctacca gaagcacgcg ccagccgaat
     7381 gtgttcatcg ccggaaaccg ggttatcact ggaaacggcc gctaaactaa gtgaagtcat
     7441 gccaggagct ggcggacgtt cctgcctgga acgctacgaa tatgccaaac acggtgcctg
     7501 ctttggtttt gatccggacg catacttcgg tacgatggta cgcctgaatc aagaaattaa
     7561 agagagcgaa gccggaaaat tccttgcgga taattacggt aaaacagtga gccgccgtga
     7621 ctttgacgcc gcctttgcca aaagctgggg aaaagagaac gtgaaagcag ttaagctaac
     7681 gtgccagggt aaccctgcgt atttgactga aattcagatc tcgatcaaag ctgacgccat
     7741 caacgctccg ctttctgcaa actcattctt gccacaacct cacccaggta actgtggcaa
     7801 aacctttgtg attgataaag cgggttatta atcctctgat ttacaaaaca cgctaattcg
     7861 tcttccataa ccatgccgat taagaattat acataatgcg ctgcattgag gaattccctg
     7921 aatgtgacgc aaatcacttc aaagagtgag caacagtcag tatcatcatg tgagttaaac
     7981 cctcgccgcc tgacggtgag ggttttcttt tgggtatgtt tatctgcgac actcgcagta
     8041 ccgacgacat ggagtaaaaa tgtccagacc aactatcatc attaacgacc tggatgccga
     8101 acgcatcgat attctgctgg agcaacccgc ctatgctggt ttgccaatcg ccgacgcgtt
     8161 aaacgcagag ttggatcgcg cccaaatgtg ttcgccagaa gagatgccac acgacgtggt
     8221 gacaatgaac agccgggtta aattccgcaa tcttagcgat ggcgaagtgc gtgtgcgcac
     8281 gctggtgtat ccggcaaaaa tgaccgatag caatactcag ctttccgtta tggctccggt
     8341 aggtgccgca ctgctggggc tgcgcgttgg cgattccatt cactgggaac ttccgggcgg
     8401 cgttgcaacc caccttgaag tgctggaact cgaataccag ccagaagctg ctggcgacta
     8461 cctgctttaa tcccatctgc acaagcgata actcgctttg cccagaggat gcactcgcat
     8521 cctctttacg caaaatcagc aatttttcct ggttctcact gttcactttt gctgcaaaat
     8581 tattccgttt cgtttgtggg aataaccatg gcagaagcat tttacatcct gataggattt
     8641 ctgattatgg cggcgattat cgtcatggct gtcctttacc tcgaaaacca ctcctgacga
     8701 tcatattgtt ccccccggca tcgcattcac cagacctgaa accgccttct gcgccgcctc
     8761 tgcgctttgt gcaccgggta ccagaatcac cttacggcac tcctcttcac gtttttcgaa
     8821 aatctcatat ccccgggcgg cctcttcaaa cggcatatag tgggtaacaa tttcttctgg
     8881 tttcagtaat cctttctcaa ttaacggtaa taattctccc agccatgcgt gaacgtgggt
     8941 ctgtcccatt ttaaacgtca accctttatc aaaggcgtcg ccaaacagga aaccgtgaat
     9001 aaatccagcg tagacgcccg gtacgctaac aatgccgcaa cgctttaccg ctgctgccct
     9061 ccagtttcag gttagtcagc accgtttccg tggtgctgcc tttcgcttca aaaccgacgg
     9121 cgtctattac tgcatccacg ccccggtgac ctgccgtttg ttcaataatt gactgtgccg
     9181 gatcgctatc ttcatcgaaa ttaatcggga tcgcgccgta gcggtcagcg gcgaaacgca
     9241 agcggtaggg atgatgatca acaacaaaaa tctgttccgc accgagcaac cgtgcacagg
     9301 cgattgtcaa caatcccaca ggaccagcac catagactgc aacgcttgaa ccttgttgga
     9361 tctgcacatt ttttgctgcc tgccatgccg ttggcagaat atcagaaagg aaaagggctt
     9421 tatcatctga aagcaaaggc ggtactttaa acggccccac attccctttc gggacgcgga
     9481 catattccgc ctgcccgcca ggaacgccac catacaggtg actataacca aacaatgccg
     9541 cgggagcagg tatctgtttt ttattgagcg cagcgccttt acccgcattg gtattttcgc
     9601 aggcggcata ttgctgtaat cgacagaaaa aacagtcgcc acaagcaatg acgaacggaa
     9661 ttaccacccg gtcgcctttt tgcaaatttt ttacgtcctt cccggtttca acgacttccc
     9721 ccataaattc atgaccaaaa atatcgccat gtttaacctg gggtattttg cctcgataaa
     9781 gatggaggtc agagccacag atcgccgtta ccgtaatacg cagaataata tcatctgcct
     9841 gttcaatgcc cggatcggga acattttcta cctgaacgtg atgtgggccg tgataagtca
     9901 atgctttcat gcaacctcca gagacagttc aaaaaagagt cagtctttaa gggtagctat
     9961 cacagcggga aaacgggcgg taccgcaggt tttgggagaa ttcctagtgt tcagtgagaa
    10021 tatattaatc acagaaaccg ttcgtaagga aaaagaacca gacaaatatc agggataaat
    10081 attataatca tggagaatga tttgttcatg attaacaggg agaaagggtt atgtataagg
    10141 caatcattat gccagttgat gtatttgaaa tggaattgag cgacaaagct gttcgccacg
    10201 ctgaattcct cgcccaggat gacggagtta ttcatctact tcacgtacta cccgggtcag
    10261 ccagcctgag cctgcaccgt tttgccgctg atgtgcgtcg ttttgaagag catctgcaac
    10321 atgaagcaga agaacgtctg caaacgatgg tcagccactt caccatcgat ccttcccgca
    10381 ttaaacaaca tgtccgtttt ggtagcgtgc gggatgaagt caatgagttg gcagaagaac
    10441 tgggggctga tgttgtagtt atcggttctc gcaacccatc gatttcgacc catctgttag
    10501 gttctaacgc ctcgagcgtg atccgccacg ccaatctgcc tgtactggtc gttcgctaac
    10561 agataaaagt atttatgccg ccctgcctgg aacatcccgg cagggctttt taatgccata
    10621 aaaaagccgc caggtttgac cccggcggct aagcaattgc aggtgaatct tacttcttct
    10681 tatgcagttt tggtgcgaat caggtagtca aaagcactca gagaggcttt ggcaccttcg
    10741 ccagtggcga tgatgatctg cttgtacgga accgtcgtac agtcacccgc tgcgaacacg
    10801 cctttgacgt tggtttcaca tttcgcatca atgataatct cgcccatgcg gttacgttcg
    10861 actgcgcctt ccagccagtt ggtgttcggc agcagaccaa tctggacaaa aataccggcc
    10921 agttcgatgt tgtgaatatt gccgctgaca cgatctcgat attccagacc aacgactttg
    10981 ctgccgtcgc ctttcacttc cgtggtttgc gcattcagaa taatgtcgac gtttttcagg
    11041 ctgcgcagtt tgtcctgcag aacctggtcg gctttcattt ctggcgcaaa ttccagcagc
    11101 gttacgtgct caacgatacc cgccaggtcg attgccgctt ccacgccgga gttaccgccg
    11161 ccgataaccg ctacgcgttt gcctttaaac agcgggccgt cgcagtgcgg gcagtaggtc
    11221 acgcctttag tgcgatactg atcttcgccc ggaacgttca tgttgcgcca ttttgcaccc
    11281 gtcgccacga taatgctgcg tgctttcagt accgcgccag aagctgtttc aatctggtgt
    11341 agaccacctt caaccgctgc cgggatcagt ttgctggcgc tctggctgtc gatcacatca
    11401 acgtcgtatt catcaacgtg aactttcagt gcgcctgcca acttctgccc ttcagtcttc
    11461 ggtacagaaa tgtagttttc gatatcaacg gtatcgagga tctgaccacc aaaacgttcg
    11521 cccatcagac cggtacggat gcctttacgt gcggagtaaa ttgctgccgc tgcacccgcc
    11581 gggccggaac caacgattaa tacgtcataa gcatcacgct tgttcagctc ttctgccgca
    11641 cgattttccg cgccagtatc aattttggca acgatttcag tcaacgtcat gcggccctga
    11701 ccaaactctt tcccgtttac gaacactgcc ggaacgccca tcacgttgcg atcggtgatt
    11761 tcgttctgga aggtgccgcc gtcaattgca gtgtgcttga tgcgcgggtt caatacgctc
    11821 atcaggttca gcgcctgcac cacgtccggg cagttatggc aagagagcga gtaataggtt
    11881 tcgaattcaa aatcaccgtc aatatggcga atctgctcca gcagagactg cgcttctttc
    11941 gacggatgac caccggtcca cagcaacgcc agtaccagcg aggtgaactc gtggcccagc
    12001 ggggagcctg caaaacgtgg tccctggttg gaacctgggt tggtgatcag gaaagacggc
    12061 ttacgcactg gcaagctgtt atcttcttta aaggtgactt tgtctgacag ttctgcgatt
    12121 tcagccaaca gttccttgat ttctgccgat ttagcgctgt catccagcgt ggcaattaac
    12181 tcaacaggct tggtcaattt ctcaaggtaa gccttgagtt gagttttcat atttgtgtcg
    12241 agcatgttta tctcctgggc tttaaacatc atcatgcaag cggcctctgc ggctacctgg
    12301 atgcagcttg catcatggtg cggggaagaa aatcgggtgc cgcagcaccc gaagaattag
    12361 gtgaatttcc gaagtatttc gcgtcagagc aaggcgacta gcctgtgaat ccccgggagc
    12421 ttacacaagt aagtgaccgg ggtgagcggg cgacgccaac gccgctatgg cgtgaaagac
    12481 gaaggaaatt tagattttac caaccaggtc cagagacgga gccagagttg cttcaccttc
    12541 tttccattta gccgggcaaa cttcacctgg gtgagaagct acgtactgag ctgctttgat
    12601 tttacgcagc agatcagacg cgtcacggcc aatgccttca gcggtaactt cgattgcctg
    12661 gatgataccc tgcgggtcaa caacgaaggt cgcacggtca gccagacctt catcttcacg
    12721 catgttgtcg aagttacggg tcagggcgcc agtcgggtcg ccgatcatcg catatttgat
    12781 tttagcgatg gtttcagagc tgctgtgcca tgctttgtgg gtgaagtgag tatcggtaga
    12841 tactgcgtat acgtctacgc ccagtttctg cagttcttcg tagtggtcag caacgtcacc
    12901 cagttcggtc gggcatacga aagtaaagtc agccgggtag aagaagaaga cgctccagcg
    12961 gccttcggta tctttttcgg tgatttcgat gaattcgccg tttttgaatg cctggttttt
    13021 aaaaggttta attttggtgt taatcaagga catctatact tcctccgtgt tttcgatgag
    13081 atgtaaggta accaattttt gccgattcgg ctaatgcgtt tccattatca aatcgataag
    13141 ttttacctta caaccttcgt aagacaactt tgtgaacttt gcaccgccag cggcgataaa
    13201 aagtaaaatg gccccctttc caggtgaccg tgtaatctga actacccttt ggtatcttca
    13261 gcgcctgcct gcaggctgag gtgttgccct tacaaatgca acaacgacat ggattacaac
    13321 accctcataa acaaagggca atcacctgat ctaagctctt acctatgaca gtgataggtt
    13381 atgcctttta ctcgactttt gcactgactg aaaagaacaa attaatgtta aaaaagatac
    13441 ttttactggc tctgcttcct gcaatcgcct tcgcagagga acttccttcc ccagtaaaag
    13501 cgattgaaaa acagggcatt acaatcatca aaacgttcga tgcccccgga ggaatgaaag
    13561 gttatctcgg aaagtatcag gatatgggcg tcaccatcta cctgactccc gatggtaagc
    13621 acgctatctc tggttacatg tacaacgaga aaggtgaaaa cctgagtaac acacttatcg
    13681 aaaaagaaat ttacgcacca gccggacgcg aaatgtggca acggatggaa caatcccact
    13741 ggctcctcga cggtaaaaaa gatgcgccgg tcattgtcta cgtcttctcc gatccgttct
    13801 gcccatattg taaacagttc tggcagcagg cgcgcccgtg ggtagattct ggcaaagtgc
    13861 aattaagaac attgttggtt ggggttatca agccagaaag cccggcgaca gcagcggcaa
    13921 ttcttgcctc caaagatccc gcaaaaacct ggcaacaata tgaagcctct ggttgcaagc
    13981 ttaagctaaa cgtgcctgca aacgtaagta cagagcaaat gaaagtgtta agtgacaatg
    14041 agaaactgat ggacgatctg ggggcaaatg tcacgccggc tatctattac atgagtaagg
    14101 aaaatacgct gcaacaggcc gtggggttgc ccgatcagaa aacgcttaat atcattatgg
    14161 gaaataaata aaaggtaatt catggatggt tgaactatat ccctgctttt caaccatcct
    14221 aaaaaagcat gaa
//
LOCUS       DAZPCS010000095        14232 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZPCS010000095 DAZPCS010000000
VERSION     DAZPCS010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14232
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.095"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..79
                     /locus_tag="ACHUQ7_002708"
     CDS             <1..79
                     /locus_tag="ACHUQ7_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1136055.1"
                     /translation="RAFPRVVKERPWKYPTAPKKSQSVA"
     gene            complement(365..982)
                     /gene="tdk"
                     /locus_tag="ACHUQ7_002709"
     CDS             complement(365..982)
                     /gene="tdk"
                     /locus_tag="ACHUQ7_002709"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFR1136056.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1587..2000
                     /gene="hns"
                     /locus_tag="ACHUQ7_002710"
     CDS             1587..2000
                     /gene="hns"
                     /locus_tag="ACHUQ7_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFR1136057.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2144..3052)
                     /gene="galU"
                     /locus_tag="ACHUQ7_002711"
     CDS             complement(2144..3052)
                     /gene="galU"
                     /locus_tag="ACHUQ7_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFR1136058.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="ACHUQ7_002712"
     CDS             complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="ACHUQ7_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFR1136059.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="ACHUQ7_002713"
     CDS             complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="ACHUQ7_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFR1136060.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5377..5835
                     /locus_tag="ACHUQ7_002714"
     CDS             5377..5835
                     /locus_tag="ACHUQ7_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFR1136061.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5885..6727
                     /gene="purU"
                     /locus_tag="ACHUQ7_002715"
     CDS             5885..6727
                     /gene="purU"
                     /locus_tag="ACHUQ7_002715"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFR1136062.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6887..6971
                     /locus_tag="ACHUQ7_002716"
     tRNA            6887..6971
                     /locus_tag="ACHUQ7_002716"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6921..6923,aa:Tyr,seq:gta)
     gene            7018..7149
                     /locus_tag="ACHUQ7_002717"
     ncRNA           7018..7149
                     /ncRNA_class="other"
                     /locus_tag="ACHUQ7_002717"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7333..8010)
                     /gene="narI"
                     /locus_tag="ACHUQ7_002718"
     CDS             complement(7333..8010)
                     /gene="narI"
                     /locus_tag="ACHUQ7_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFR1136063.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="ACHUQ7_002719"
     CDS             complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="ACHUQ7_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFR1136064.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQVQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8717..10255)
                     /gene="narH"
                     /locus_tag="ACHUQ7_002720"
     CDS             complement(8717..10255)
                     /gene="narH"
                     /locus_tag="ACHUQ7_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFR1136065.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10252..13995)
                     /locus_tag="ACHUQ7_002721"
     CDS             complement(10252..13995)
                     /locus_tag="ACHUQ7_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFR1136066.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGYLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
BASE COUNT         3522 a         3754 c         3408 g         3548 t
ORIGIN      
        1 aagggccttc ccgagagtgg taaaggagag gccctggaaa taccccacag ctccgaaaaa
       61 gagccagtca gttgcttaac tgactggcat taccccttct ggggccgttt ttttgtttac
      121 ccaaagcaac ttttccataa accgacagca ttagccttca tcatatttgc gacgatgtat
      181 aacgcctaaa cacagggata ttgtacttta caggtcacaa gtcaacgtcg gtgcttaaga
      241 gccctgtgag gcgtatagcg gcgttaaaaa actgctgaga agggtatata gcccggaaga
      301 agtgcgtaaa acgaactgac aggataaaag tgccctgctc accctgtcag taaagaaatt
      361 cttattaatc gtggcgatgc ctttcctgaa tagccgttaa tgagccgact tgtaacgcct
      421 ctttatagtg tttacggcat acagaaacgt atcgttcatt accaccaatt accacctgct
      481 caccttcgtt ataaggtctg cctgcttgat caagacgcag caccatgctt gctttacggc
      541 cacaaaaaca gatggttttt aattcaacca gtttgtcgga ccatgccagt aagtattggc
      601 tgccaataaa taattcacct cgaaaatcgg tacgtaaacc ataacaaagt acgggtatat
      661 cgagttgatc gacaacctcc gataattcat atacttgttg tctggttaaa aactggcatt
      721 catcaaccag tacgcaatga attgcctgct gttcatgttc cgcacgaatc tcatcaaata
      781 atgatgaatt ttggttaaat aattttgcag gcgatgacaa acctatacgc gaactgactt
      841 tcccggcacc aaagcgatca tcaatttctg ccgtatatac gacagtgcgc atgccgcgtt
      901 cctggtaatt gtatgaagat tgcaacaatg ctgtagactt acccgcattc attgcggaat
      961 agtagaaata tagctgtgcc atcagccaca ggccctcaat gatatgaaat aaagttggct
     1021 ggagtttatc ataattcgtc agtttttcag ctcaacagta tgcacagaag tgggtatatg
     1081 cgttctccct tacgaagcct tgcataatcc ttctgagcta tcattacaac tgcctcgctt
     1141 gttataagcg ggtaaatgac tgctggtaac tattcacaat ctttaacctg ttgcgcaagt
     1201 aatagccctc tgttgacctc caggagatag tgcaatacta agtccatgct cttattgcga
     1261 ctgttctact tttcatcatt cgcttaatag ggaattctcg taaacacaac taatacagaa
     1321 gactgaaagg tcgtcagcct acgataatct ccccataaaa tgtgacatga atcaggaagt
     1381 tttaacctca cgtgctgcga aatcatcggt gtaaataggg ctatatgccg cgtcttttct
     1441 ggctaatttt atgaaaagat atttattggc ggcacaaaat aaagaacaat tttgaattcc
     1501 ttacattcct ggctattgca caactgaatt taaggctcta ttattacctc aacaaaccac
     1561 cccaatataa gtttgagatt actacaatga gcgaagcact taaaattctg aacaacatcc
     1621 gtactcttcg tgcgcaggca agagaatgta cacttgaaac gctggaagaa atgctggaaa
     1681 aattagaagt tgttgttaac gaacgtcgcg aagaagaaag cgcggctgct gctgaagttg
     1741 aagagcgcac tcgtaagctg cagcaatatc gcgaaatgct gatcgctgac ggtattgacc
     1801 cgaacgaact gctgaatagc cttgctgccg ttaaatctgg caccaaagct aaacgtgctc
     1861 agcgtccggc aaaatatagc tacgttgacg aaaacggcga aactaaaacc tggactggcc
     1921 aaggccgtac tccagctgta atcaaaaaag caatggatga gcaaggtaaa tccctcgacg
     1981 atttcctgat caagcaataa tcttttgtag attgcacttg cttaaaatcc cgccagcggc
     2041 gggatttttt attgtccggt ttaagacaat ttaataagaa taagctataa aaaaacggcg
     2101 tcgattgctc aacgccgttt cgtggataac accgatacgg atgttacttc ttaatgccca
     2161 tctcttcttc aagccaggct ttaaattccg tgccaagggt gttatgacga ataccgtatt
     2221 caacgaaggc ctgcatgtaa cctaatttat taccgcagtc atggctcttc cctttcatat
     2281 gataggcttc caccgtttct ttttcgatca gcatatcaat tgcgtcggtg agctgaattt
     2341 catcaccagc tcccggaggg gtttttgcca gcaacggcca aatatccgcg ctaagtacgt
     2401 aacgacccac aatagcgaga ttagacggcg caacatccgc tttcggtttt tctaccacac
     2461 caaccatcgg tacgctttca cccggcgcta attcaacgcc tttgcaatcc acaacgccat
     2521 atgcggtcac atcagcaacc ggttcaacca tgatctggct atgacccgtt tcatcaaagc
     2581 ggcggatcat ctctgccagg ttatcctgtg acaaatcgga ttcatattca tccagaataa
     2641 catcaggcaa aataacagct accggttcat cacccactac cgggtgagca cacaataccg
     2701 cgtgtcccag gcctttcgcc agaccctgac gaacttgcat aatagtcacg tgcggtggac
     2761 aaatagactg cacttcatca agcagttgac gttttacacg tttttccagc attgcttcca
     2821 gttcaaaact ggtatcaaag tggttttcaa tagagttttt agatgagtgt gtaaccagca
     2881 caatttcagt aatgccagcc gcaatacatt cattcacgac gtattgaatt aatggcttat
     2941 cgacaagtgg cagcatctct ttcgggatgg ctttcgtcgc cggcaacatc ctggttccta
     3001 atcccgcaac ggggataacg gcttttttga ctttcgtatt aatggcagcc atttaaattc
     3061 tcctggactg ttcgtgtttt gaacgtgttc atatttctgt atcgcatccc agtatatcag
     3121 caccccgaag gggctaagtt ctgaaaagga ggcgagatag cataattaag acaattactt
     3181 atacgaatgg cgacgatagt accaccagcc gacattagca ggtaatgcaa atttagcccg
     3241 cgttatcgtt tgctcattct gcagacaaca tcaagcgcag tcgaccaccg gttccccata
     3301 tttggcattg ccaggcatcg catcgctggc tcaattgatt caaataagcg ttacctaaag
     3361 tgcctaacgg aacaccatta ctgatttgca cctggtgttc gccggtattt aacgtcgcat
     3421 tcagacccgc agaaaccaga atgagatttt tcagttcgcg atgataatag ccaactaata
     3481 gcggaaactg ccccggcaga ttggcctgac gaagtaaatg gtttacctgc ttcaataacg
     3541 cgcccaactc tggcaaccgt tgattttggt gtgcaagctg ttcctgtaat aatccgttaa
     3601 acaatgcgcg taataacaag gcagcaagta cgccattatg tccagctcgg gtgacatcaa
     3661 ggcaataaaa tgccagatcg ttttccgaaa gtgcggcaat atcaagcacc aggccgggtt
     3721 tgtccgcggc aaccaattga cggtaattaa cccggcaatg ggaaatcacc tgctgaaccg
     3781 gcggttgtag ttcctgtaat aatttcgccg ctgcggcagg gttatcaacc attgcatccc
     3841 agtcgcgaaa aagcctttcc tcttcctcaa cgcgcgaatt aaacatgctg ggatagagac
     3901 aggcaaaaac catctcgcgc aagcgattca gatctttaac tggtttcagc aaaacatctt
     3961 caacgcccag acgtaacgct ttggcaatat ctgccatatt ttcagtggca gatatcacca
     4021 gaactggggt ctggtcgcct ctgttacgta tatgctccag cagtttaagc ccgttcattc
     4081 gtggcatcgc gatatcacat atcatcaggt ctggagtgaa acctcccagc aactcaaggg
     4141 catccacccc atcagccgcc agtaccgttg tcgctcccaa tgaggaaaac catgaatcca
     4201 gaagcgagcg aaatacctgc tcatcttcaa caatgagaat ctgttttccg accaatggct
     4261 gcgtcatgtt ctctcccctg actggcttta ctcaatagtg gcatgctatt gccacctgcg
     4321 cctgtcagaa ttaacttaag tgtagataaa aattctggtc aaatgttggt gcgtaccaac
     4381 ggcaaaagtt cgtccatttt cctttccact gccagctgtc cggccgcaat ggcagcgtgc
     4441 gcgcgatgga aatcaagcgt agatatttgc gggcaaacag gttgaatcag aatatcgggc
     4501 ggatcacctg ccatgcggtt ccttttaagg cggttctcca acacctggat agacgtggtc
     4561 ataatctctg ttgccgttgg cgctgtcaca gcccgacgtg tcgttatgct gcctaacctt
     4621 tctttcagac gcgcatgcca cggtagagaa tcaccattct cgctattttc ttcactgaca
     4681 ttaaaggaga gcagatcttg ttgcatcaaa tgagcatcgt gctgcaggtc gaccgctatc
     4741 acaatatcag cccccaatgc acgcgtaaga gaaataggaa ttgggttaac tactgcccca
     4801 tcaaccagcc agtagccgtt atgtgcaacg ggtgccatga gtcctggaat actgcatgat
     4861 gcgcgaatag caagatggag atcgccttca gtaaaccata attcacgccc cgtacttaaa
     4921 ttggtggcaa cagccgcaaa gcgacgggaa caattttcga tctctgtttc cggcattatt
     4981 tcgcgatatt gattgaagac acgctcgccg cgcagtaacc caccgcgctg ccaggagagg
     5041 tccatcaggc gtaaaacgtc ccaataactg aaagaggtta cccaatcttc cagcgcagat
     5101 aatcgatcgc atgcataggc cgcgcccacc agcgaaccaa ttgaacatcc tgcaacgata
     5161 tcaatttcaa tacccacttt ttttagcgca ttaataacgc caatatgcga ccaacctctc
     5221 gccgcgccag atcccagcgc cagccctatt tttatctttc tcattattcc cgcaagattc
     5281 ccctggaatg caatcgtagc cacatcgcta ctgctcggtt aacatagtgc tactcaggcg
     5341 gttaatacgc cgttattgtt tccagggaga ttatttgtgt ctcagctttg tccctgtggt
     5401 agtgctgtcg agtatagcct atgttgccac ccttatgtgt ctggtgaaaa ggttgcacct
     5461 gatccagaac atctcatgcg ttcgcgttac tgcgcttttg tgatgcaaga cgctgattat
     5521 ttaataaaga cctggcatcc ctcttgtgga gcagcggcgt tacgtgccga attgatgacc
     5581 ggatttgcgc atactgaatg gttgggattg accgtatttg aacattgttg gcaggacgcc
     5641 gacaatatcg gatttgtcag ttttgttgct cgatttaccg aaggtggcaa aacaggtgca
     5701 ataattgaac gttcgcgatt tttgaaagaa aacggtcaat ggtactatat tgacggtaca
     5761 cgtccgcagt ttggtcgcaa cgatccctgc ccttgtggtt caggtaaaaa atttaaaaag
     5821 tgctgcggcc aataatggtt gacggtacgg tttagcaaac actctcaaca aggtttttcc
     5881 agcaatgcat tcactccaac gtaaagttct gcgtactatt tgtccggacc aaaaaggtct
     5941 gatcgcacgt attaccaata tttgctacaa gcacgagtta aatatcgtac agaacaatga
     6001 atttgttgat caccgtaccg ggcgcttttt tatgcgcacg gaactggaag ggatttttaa
     6061 tgattccacc ctgctggcgg atctcgatag cgcattgcca gaaggctccg tgcgtgagct
     6121 gaatcctgcc ggtcgtcgcc ggatagtgat tctggtcact aaagaagcgc attgccttgg
     6181 cgatttgttg atgaaagcca attacggcgg tctggatgtc gaaatcgcgg cagttattgg
     6241 taaccacgat actttacgtt ctctggttga gcgttttgat attccgtttg agctggtaag
     6301 ccatgaaggg ttaagccgca acgagcacga tcaaaagatg gcggacgcca ttgatgctta
     6361 tcaacctgac tatgtggtgc tggcgaagta tatgcgggta ttaacgccgg aatttgtggc
     6421 acgcttcccg aataaaatca tcaatattca ccattcattc ctgcctgcgt ttatcggcgc
     6481 acgtccttat caccaggcct atgaacgtgg tgtgaagatt attggcgcaa ccgctcacta
     6541 tgtgaatgac aatctggacg aaggcccaat catcatgcag gacgttattc atgtcgatca
     6601 tacctacaca gctgaagata tgatgcgcgc aggtcgtgac gtcgagaaaa acgtcttaag
     6661 tcgtgcgcta tacaaagtac tggcgcaacg cgtctttgtt tacggtaatc ggacgattat
     6721 tctttaatcg ccagcaaaaa taactggtta cctttaatcc gttacggatg aaaattacgc
     6781 aaccagttca tttttctcaa cgtaacactt tacagcggcg cgtcatttga tatgatgcgc
     6841 cccgcttccc gataagggag caggctagta aaaagcatta ccccgtggtg gggttcccga
     6901 gcggccaaag ggagcagact gtaaatctgc cgtcatcgac ttcgaaggtt cgaatccttc
     6961 ccccaccacc atcactttca aaagccccgg aattctcaaa cgaatctgca atcaaatatt
     7021 ctgcccaagc ggggaaggat gagaagcttc gaccaaggtt cgacttgagc gccagcgaga
     7081 gagcgttgcc gcaggcaacg acccgaaggg cgaagcgcgc agcgctgagt aatccttccc
     7141 ccaccaccat cgcttccttg aataatcaaa acctgttact ccgagctaat tttgcccgat
     7201 ggcaccacgt ttaccgggcc tacaatatcc tcccctgtag gtcggataag cgaagcgcat
     7261 ccgacaactc accccgcacc gtgcccataa aaaaaccccg ccgaagcagg gtctatgtga
     7321 actaaaattc gtttagtgac gagcgcgcac cagctggtac ttacgtgtca gatactccac
     7381 cggtacgctc cagatgtgta ccagacgcga gaacgggaac agcaggaata acgtcatccc
     7441 cagcaccagg tgcagacgga agataaacgc cacaccatcg aggtgttgag aagcgccacc
     7501 gtggaaggtc accaccgact gcgcccagcc aaccagtttc atcatctcgc taccgtccat
     7561 atgctgagcg gagaacggaa tggtcagcag gcccagcgcg cactggataa cgagcagcga
     7621 caggatcagg atatccgctc cggtagtggt tgcacgcacg cgtgggctga acatacgacg
     7681 tttcagcaaa agcacgccgc caatcagaca cagcacgccg ctggcaccac cagcaaacat
     7741 cgccattttc tgtttcactt caatcggcag ccaggcttca tacatccagt gcggcgtcaa
     7801 cataccgaag aagtgaccga caaaaatccc cagaataccg atatggaaca ggttcgacgc
     7861 caggttcatc cctttgcgat ccagcatctg gctggacgcc gcgcgccagg tgtactgccc
     7921 gtagtcataa cgcagccagc taccaatcag gaagaccgcc ccggctatgt acgggtagat
     7981 atcaaagaag aacatattca ggaattgcat tagtgctgtc ctccggtggt gatattcaga
     8041 tattgcggcg cgacggcacc ggcaaagcga cgctgatgag cagtgattgc tgaatcaccg
     8101 cagcctttgt cagcaaagaa tttaacctgc tcttcttccc agacagcatc cagcgcctgc
     8161 ggcgtatcat cgcgcgcttc gtcggcaatt ttttccgcca ctttgtcgct gtcgatagcg
     8221 gtattggcca atttcagcag cagatcaaac atcacggcat aacggctttc acgctgttgc
     8281 agacgcgcgc tcagcaatgc cagaatcggc gcgatatctt tcaaaccttc cacggcttcg
     8341 ctttgcggca gctgcgacag gtactccaga tacagcggca gatggtccgg taattcgcgg
     8401 ctgtttaact gcaagccgtg ctgctcgtac tgcgccagca ggtccaccat cgcctggccg
     8461 cggtcacggg attcaccgtg aacatgttca aatagcaaca gtgacgtggc acggccacgg
     8521 tcgaacagtt cgctgtactg gacctgggca tcgagcggat ccatcgccgt taaatcgcgc
     8581 aggaaaatgc ccagcgcatg ggcatcttct ttcgacagat ttttcgacgc agcaatcgcc
     8641 tcaaacatct cttgttgatg ctgccataag gcagcatccg gatattcaag gagacgcgat
     8701 acaatcacga gttcgatcat ggatgcggct ccgttttgct ggtcacatcg atggcatcga
     8761 tacgacggct gttgaacaga ttgaatttgg tatctgagcc gtggcagcca tcaccaaagg
     8821 taaagccgca gccatttttc tccgggaagg cttcccgtgc cagttcacga tgactactcg
     8881 gcaccacaaa gcgatcttcg tagttagcaa tcgccagata acggtacatc tcctgtgcct
     8941 gggcttcggt cagaccgacc tcttccagcg cacgggtatc aactttaccg tcaacagttt
     9001 cagcacgttt gtaatgacgc atcgccagca tacgtttcag cgcacgcagt accggtttgg
     9061 tatcaccggc ggtcagcaga ttcgccagat actgtaccgg aatacgcaag ctttcgacgt
     9121 ctggcagaat tccgttgctg cccagctcgc ccgcgtctgc tgcagactga atcggagaca
     9181 gaggcggcac gtaccagacc atcggcagtg tgcgatattc tggatgcagc ggcagcgcca
     9241 gtttccattc catcgccatt ttataaaccg gcgactgctg tgcagcttca ataacgctca
     9301 gcggaatacc gtctttaatc gcctgctcga tgactttcgg atcgttcgga tcgaggaaca
     9361 cgtccagctg acgctggtag agatctttct cgttctcggt gctggctgca cgttcaatcg
     9421 cgtcggcatc gtacagcagc acgccaagat aacggatacg accgacacag gtttctgagc
     9481 acacggtcgg ctgacccgct tcaatacgcg gatagcagaa gatgcacttc tcagacttac
     9541 cgctcttcca gttgaagtag atttttttgt acgggcatcc ggtgatgcac atacgccagc
     9601 cacggcattt atcctggtcg atcaggacga tgccatcttc ttcacgcttg taaatcgcac
     9661 cgctcgggca ggtcgccaca catgccgggt tcaggcagtg ttcgcacagg cgcggcaaat
     9721 acatcataaa ggtgttttcg aactggctat acatcgcctt ctggatgttg tcgaagttct
     9781 tgtctttcgc cagtttatca aactcaccgc ccaggtcatc ttcccagttc ggcccttttt
     9841 cgattttcgc catccgttcc ccggtaatca gcgaacgcgg acgggcaatc ggctgcgatt
     9901 tgctgccttc cggcgcggta tgcaggttct gataatcaaa atcgaacggc tcgtaataat
     9961 cgtcgatccc cggcagatgc gggttagcaa agattttacc cagcagcatg gctcggttac
    10021 ccatgcgcgg ttgcagtttg ccattgattt tacggatcca gccgcctttg tatttttcct
    10081 ggttttccca gtcagtcggg aagccctggc ccggcttggt ttccacgttg ttgaaccacg
    10141 cgtattccac gccttcacgg ctggtccaga cgtttttaca ggtaactgaa caggtgtggc
    10201 agccgatgca cttatcgaga ttcagcacca tgccgacttg tgaacgaatt ttcattttac
    10261 gctctcctgt acctggtcat tgccttcgcc atctaaccag tcaatgttct tcatcttacg
    10321 cactacaaca aactcatcgc ggttagaacc tacggtgccg tagtagttaa agccgtatgc
    10381 cagatgggca tagccgccga tcatatgcgt cggtttcggc gtgatacggg tgaccgagtt
    10441 atggatacca ccacgctgtt gggtaatttc cgaaccaggc aggttaacga tacgttcctg
    10501 cgcgtggtac atcatggtca tccctgccgg aacacgctgg ctgacaaccg cacgggcagt
    10561 cagtgcaccg ttgctgttga agacttcaat ccagtcgtta tcggcgatac ccagatcttt
    10621 ggcatcggct tcactcaacc agaccaccgg accaccgcga cctaaagtca gcatcagcag
    10681 gttgtcgctg taggtggagt ggatacccca cttctggtgc ggcgtcagga agttgagcgc
    10741 tttttccggg ttgccgttgg atttctggcc tatcacttct ttcaccgaac gggtgtcgat
    10801 cggcggacga taaaccagca ggctttcacc gaaatcacgc atccactggt gatcctgata
    10861 cagttgctga cgaccagaga gcgtacgcca tgggatcagc tcgtgaacgt tggtgtaacc
    10921 ggcgttgtaa gaaacgtgtt catcttccag accagaccag gtcgggctgg agataatttt
    10981 gcgcggctgt gcctgaatat cgcggaagcg gatcttctcg tcttctttat tcagcgccag
    11041 atgcgtatgg tcacgaccgg taaattcgct cagggcagcc caggctttca cggctacctg
    11101 accgttggtt tccggtgcca gcgtcaggat catctctgcc gcatcaattg cggtgttcag
    11161 catcggctgg ccttttgccg gaccttccgc tttggtgtag ttgagcttac gcagcagatc
    11221 catctcgctc tgggtgttcc aggcaatccc tttaccgccg ttaccgattt tctccatcag
    11281 cgggccgata gaggtaaagc gttcgtaagt tgccggataa tcgcgctcta cgaccataat
    11341 gtgcggtgca gttttgcctg ggatcaggtc gcattcgcct tttttccagt ctttcacatc
    11401 cagcggctgc gccagttcag cggcagagtc gtgctggata ggcagcgtga cgatgtcggt
    11461 ttctttaccc agatagccga cgcacacttc ggagaatttc ttcgcgatgg ctttgtagat
    11521 ttcccagtcg cttttcgctt cccaggccgg atcgaccgcc gcagacagcg ggtgaataaa
    11581 cggatgcata tccgaagtat tcatgtcgtc tttttcgtac caggtcgccg tcggcaaaat
    11641 gatgtcggaa tagagacagg tgctcgacag acggaagtcc agcgtaacca ccagatccag
    11701 cttgccttcc agaccattgt cctgccagtc cacttcttcc ggcttcacgc cgccctgttg
    11761 ccccagatct ttaccctgga taccgtgctc cgtccccagc aggtacttga gcataaactc
    11821 atgaccttta ccggaagaac cgagcaggtt agaacgccag atgaacaggt tgcgcgggtg
    11881 gtttttaccg ttttctggtt gttctgccgc aaaacggatg gaaccctctt tcagggattt
    11941 caccgtatag tccaccggat tcatcccggc tttttccgct tcgccagcga tagtcagcgg
    12001 gttagtgcct aactgcggtg cagacggcag ccagcccatg cgttccgcac ggacgttaaa
    12061 gtcgatcaag tgtccggtat agcgggattt gtccgccatc ggtgacagca actcttccgc
    12121 cgtgacggtt tcataacgcc actggctgga gtggttatag aaataagaag tgctgttcat
    12181 gtgacgcgcc ggacgctgcc agtcaagggc aaacgccagc ggctgccagc cggtttgcgg
    12241 acgcagtttt tcctgaccta catagtgcgc ccagccgccc ccgctctgac cgacacagcc
    12301 gcagaaaatc agcatgttga tcagaccacg atagttcata tcgaggtgat accagtggtt
    12361 cagccccgca ccgacgataa tcatcgaacg accgtgcgtt ttatcagcgt tatcggcaaa
    12421 ttcacgggcg atgcgaataa tctggctgcg agaaacgccg gtaatctgct cggcccaggc
    12481 cggggtataa gctttcacat cgtcatagct ggttgcacag ttaacgtcgt tcaggccacg
    12541 ttccagaccg tagtttgcca gcgtcagatc ataaacggtg gtcaccaggg cggtgctgcc
    12601 atcagccagt tgcaggcgtt tcaccggcag tttgtgcagc agcacgtttt ccagttccac
    12661 tttgttgaag tgttccgtgc cgtcgccacc aaagtacggg aagcctacct cagcgatctc
    12721 atcctggcta cccagcaggc tcagttgcag ctcggtttct tcgccagttt tgccgtcgcg
    12781 ctgctcaaga ttccatttgc ccttctcgcc ccagcggaag ccaatagaac cgttcggcgc
    12841 aaccatttcg ccattggtat taaaggcgac agttttccat tccggattgt tttcctggcc
    12901 cagcgcatca accagatcag cagcgcgcag catacgacct gcagcgtagt aaccgtcgcg
    12961 ttcttccagc atcaccagca tcggcatgtc ggtgtagcga cgcacatagt cggtgaaata
    13021 ctggcttggg ttgtcgaggt ggaattcacg cagcattacg tggcccatcg ccagcgccat
    13081 tgccgcatcg gtgccctgtt tcggtgccag ccacagatcg cacagtttgg cgatttcagc
    13141 gtagtctggt gtgacggcaa cagttttggt gcctttgtaa cgcacttcag taaagaagtg
    13201 agcatccggg gtacgcgtct gcggcacgtt tgaaccccag gcgatgatgt agctggagtt
    13261 ataccagtca gcagattccg gtacgtcagt ttgctcgccc caggtttgcg gagacgcagg
    13321 aggcaggtcg cagtaccagt cgtagaagct taagcaagta ccgccaatca gcgagagata
    13381 gcgtgcaccc gatgcgtaag aaaccatcga cattgccgga attggcgaga aaccagcaac
    13441 acggtccggg ccgtagtttt tgatggtgta aacgttagat gcggcgatca gttcgttcac
    13501 ctcctgccaa gaagaacgaa caaatccacc gcgtccacgc gcctgcttaa agcttttcgc
    13561 tttatcggcg tcttcaatga tagaagccca tgcctcaacc ggatcgctat gcagcgcctt
    13621 cgcttcacgc cacattttca tcaggcgttt gcgcatcatc gggtatttca ggcggttggc
    13681 actgtaaaga taccaggagt agctggcacc gcgcgggcag ccgcgaggtt catggtttgg
    13741 cagatccgga cgggtacgcg gatagtcagt ctgctgggtt tcccaggtga ccagaccgtt
    13801 tttgacgtag attttccagc tgcaggagcc ggtgcagttt accccgtggg tagagcggac
    13861 gattttgtca tgctgccaac gctggcgata tccatcctcc cagtcacggt tggtattgag
    13921 aagctggcca tgcccatcgg caaaggtttc acccttctgt ttgaagtagc gaaaccggtc
    13981 caggaattta ctcatcggtt ttctcctgtg ggagcctgtc ggccctctga taaatcgaca
    14041 ttgctaatgt ttaagggcaa ggtaacgctc tgaaacagcg tgggaattga taacgatcaa
    14101 gagtgatggg gatgaaaaat aaagtaaatt cctaagatac ccctttagga taattaacag
    14161 ttacagcata acatgctgta aattaagacg ttttatacct atagttattt aaactattaa
    14221 gatttttatg ca
//
LOCUS       DAZPCS010000096        14102 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZPCS010000096 DAZPCS010000000
VERSION     DAZPCS010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14102
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.096"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            6..407
                     /locus_tag="ACHUQ7_002722"
                     /pseudo
     CDS             6..407
                     /locus_tag="ACHUQ7_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            572..4690
                     /gene="pic"
                     /locus_tag="ACHUQ7_002723"
     CDS             572..4690
                     /gene="pic"
                     /locus_tag="ACHUQ7_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Pic"
                     /protein_id="HFR1136067.1"
                     /translation="MNKVYSLKYCPVTGGLIAVSELARRVIKKTCRRLTHILLAGIPA
                     ICLCYSQISQAGIVRSDIAYQIYRDFAENKGLFVPGANDIPVYDKDGKLVGRLGKAPM
                     ADFSSVSSNGVATLVSPQYIVSVKHNGGYRSVSFGNGKNTYSLVDRNNHPSIDFHAPR
                     LNKLVTEVIPSAVTSEGTKANAYKYTERYTAFYRVGSGTQYTKDKDGNLVKVAGGYAF
                     KTGGTTGVPLISDATIVSNPGQTYNPVNGPLPDYGAPGDSGSPLFAYDKQQKKWVIVA
                     VLRAYAGINGATNWWNVIPTDYLNQVMQDDFDAPVDFVSGLGPLNWTYDKTSGTGTLS
                     QGSKNWTMHGQKDNDLNAGKNLVFSGQNGAIILKDSVTQGAGYLEFKDSYTVSAESGK
                     TWTGAGIITDKGTNVTWKVNGVAGDNLHKLGEGTLTINGTGVNPGGLKTGDGIVVLNQ
                     QADTAGNIQAFSSVNLASGRPTVVLGDARQVNPDNISWGYRGGKLDLNGNAVTFTRLQ
                     AADYGAVITNNAQQKSQLLLDLKAQDTNVSEPTIGNISPFGGTGTPGNLYSMILNSQT
                     RFYILKSASYGNTLWGNSLNDPAQWEFVGMNKNKAVQTVKDRILAGRAKQPVIFHGQL
                     TGNMDVAIPQVPGGRKVIFDGSVNLPEGTLSQDSGTLIFQGHPVIHASISGSAPVSLN
                     QKDWENRQFTMKTLSLKDADFHLSRNASLNSDIKSDNSHITLGSDRAFVDKNDGTGNY
                     VIPEEGTSVPDTVNDRSQYEGNITLNHNSALDIGSRFTGGIDAYDSAVSITSPDVLLT
                     APGAFAGSSLTVHDGGHLTALNGLFSDGHIQAGKNGKITLSGTPVKDTANQYAPAVYL
                     TDGYDLTGDNAALEITRGAHASGDIHASAASTVTIGSDTPAELASAETAASAFAGSLL
                     EGYNAAFNGAITGGRADVSMHNALWTLGGDSAIHSLTVRNSRISSEGDRTFRTLTVNK
                     LDATGSDFVLRTDLKNADKINVTEKATGSDNSLNVSFMNNPAQGQALNIPLVTAPAGT
                     SAEMFKAGTRVTGFSRVTPTLHVDTSGGNTKWILDGFKAEADKAAAAKADSFMNAGYK
                     NFMTEVNNLNKRMGDLRDTNGDAGAWARIMSGAGSADGGYSDNYTHVQVGFDKKHELD
                     GVDLFTGVTMTYTDSSADSHAFSGKTKSVGGGLYASALFESGAYIDLIGKYIHHDNDY
                     TGNFASLGTKHYNTHSWYAGAETGYRYHLTEDTFIEPQAELVYGAVSGKTFRWKDGDM
                     DLSMKNRDFSPLVGRTGVELGKTFSGKDWSVTARAGTSWQFDLLNNGETVLRDASGEK
                     RIKGEKDSRMLFNVGMNAQIKDNMRFGLEFEKSAFGKYNVDNAVNANFRYMF"
     gene            complement(4841..5083)
                     /locus_tag="ACHUQ7_002724"
                     /pseudo
     CDS             complement(4841..5083)
                     /locus_tag="ACHUQ7_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025243830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(5122..6072)
                     /locus_tag="ACHUQ7_002725"
                     /pseudo
     CDS             complement(5122..6072)
                     /locus_tag="ACHUQ7_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(6123..6698)
                     /locus_tag="ACHUQ7_002726"
                     /pseudo
     CDS             complement(6123..6698)
                     /locus_tag="ACHUQ7_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            6702..7102
                     /locus_tag="ACHUQ7_002727"
                     /pseudo
     CDS             6702..7102
                     /locus_tag="ACHUQ7_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7505..8086)
                     /locus_tag="ACHUQ7_002728"
     CDS             complement(7505..8086)
                     /locus_tag="ACHUQ7_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1136068.1"
                     /translation="MYYPVTDYIALALIISFLFLTLFICLLCLKHERIKKETIRQKNA
                     HILEHGWNATEFSWFRYGQYNETGIYISIEKTIIITITVSGGCFKKEYSIVSHMLVTD
                     TITEATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKNMTLRLRLQDQQEETSVTLF
                     QGKMSTDGNNYYIIKGKVSSVLLLLKMLQINHA"
     gene            complement(8133..11990)
                     /gene="sigA"
                     /locus_tag="ACHUQ7_002729"
     CDS             complement(8133..11990)
                     /gene="sigA"
                     /locus_tag="ACHUQ7_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HFR1136069.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFISSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGVLTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEH
                     TTWTLTGYKVAENTASSGAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWARI
                     MSGAGSASGGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAINANFRYSF"
     gene            complement(12351..13355)
                     /locus_tag="ACHUQ7_002730"
                     /pseudo
     CDS             complement(12351..13355)
                     /locus_tag="ACHUQ7_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            13340..13717
                     /locus_tag="ACHUQ7_002731"
                     /pseudo
     CDS             13340..13717
                     /locus_tag="ACHUQ7_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839879.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(13756..>14102)
                     /locus_tag="ACHUQ7_002732"
     CDS             complement(13756..>14102)
                     /locus_tag="ACHUQ7_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR1136070.1"
                     /translation="VPGEYSSGNSIRPRGITKVGNSELRRLLYEAAWSYRTPAKVGAW
                     LIYYRPDSVTQYSKDIAWKAQQRLCSRYRTLTAKGKKSQVAITAVARELTGFMWDIAL
                     AAQSSFSQQKQN"
BASE COUNT         3540 a         3315 c         3457 g         3790 t
ORIGIN      
        1 ggtgacgagt ctcatcgtct atggaaacga cattcgtgct ggatgcactg gagcaggcgt
       61 tgtgggcccg tcgtccgtct ggcaccatcc atcacagcga taaaggctct cagtatgtgt
      121 cactggccta taaggcgcga ctaaaagaag caaaactgct ggcatcaaca gggagtactg
      181 gtgactcgta tgacaacgcg atggcagaga tcatcaaggg cctttacaaa gcggaggtaa
      241 tacaccgtaa gagctggaaa aaccggacag aagtggagct ggccacactc acgtgggtgg
      301 actggtataa caatcgacga ttgctggaaa ggctgggcca tatcccaccg gcagaagcag
      361 aaaaagctta ttatgcttcc atcggaaatg atgatctggc agcctgagtt cacagataaa
      421 acactctcca ggaaacccgg ggcggttcag ttcacaaaaa cacattaatg cagtaactat
      481 attttccttt ctggtgataa cgtcgggtta tcattagctt cttcagctat tttactttta
      541 tatcccttgt aaacatcatg gagaatccat agtgaataaa gtttattctc ttaaatattg
      601 ccccgtcacc ggggggctta ttgctgtctc tgaacttgcc cgcagggtaa taaaaaagac
      661 atgccgaaga ttaacgcata ttcttctggc tggcattcca gcaatctgtc tgtgttactc
      721 tcagatatcc caggcgggta ttgtccgttc cgatattgcc tatcagattt atcgtgattt
      781 cgccgaaaac aaagggcttt ttgtacctgg tgccaatgat attccggtat atgataagga
      841 cggaaaactt gtgggaagac tgggtaaagc cccaatggcc gatttcagca gtgtgagctc
      901 aaatggcgtt gctacgcttg tatcgcctca gtatatcgtc agcgtaaagc ataacggagg
      961 atatcggagt gtgagctttg gtaatgggaa aaatacatat tcccttgttg accgtaataa
     1021 ccacccttct attgacttcc atgctccacg tctgaataaa ctggttacag aagttattcc
     1081 ctcagcggta acatcagaag gaaccaaagc caatgcttat aaatacactg aacgttacac
     1141 cgctttttat cgggtgggta gtggtacgca gtacactaag gacaaggacg gaaatttagt
     1201 taaggttgcc ggtggatatg cttttaaaac aggaggaacc acaggagttc ctctgatatc
     1261 tgatgcaaca atagtctcta atcccgggca aacttataat cctgtaaacg gccctttacc
     1321 tgactatgga gcccctgggg atagtggttc tcctttgttt gcttatgata aacaacaaaa
     1381 aaaatgggtt attgttgctg tattaagagc atatgcaggt attaatggtg ctacgaactg
     1441 gtggaatgtc ataccaacag attatctgaa ccaggttatg caggacgatt tcgatgcccc
     1501 cgtagacttt gtttccggac tgggccccct gaactggaca tacgacaaaa catcaggcac
     1561 aggtaccctg agccagggca gtaaaaactg gaccatgcac gggcagaaag acaatgacct
     1621 caatgccggt aaaaatctgg tattcagcgg gcagaatggt gcaattatcc tgaaagacag
     1681 tgtgactcag ggtgccggtt atctcgaatt taaagacagt tacaccgtat ctgctgaatc
     1741 cggaaaaaca tggacgggtg ccggcattat tactgacaag gggacgaatg taacctggaa
     1801 ggtcaacggc gttgccggtg acaacttgca taagctgggg gaaggaaccc tgaccataaa
     1861 cggaacaggt gtaaacccgg gaggactgaa aacgggagac ggtatcgttg tacttaacca
     1921 gcaggcagac actgcaggta atatccaggc cttcagttca gtgaacctcg ccagcggacg
     1981 tccgaccgtg gtgctcgggg atgcccgtca ggtcaatccg gataacattt catggggata
     2041 ccggggaggt aagcttgacc ttaatggtaa tgccgttacc ttcacccgac tgcaggctgc
     2101 tgattacggg gcggtgatta caaataatgc acagcaaaaa tcccagcttt tactggatct
     2161 taaggctcag gatacaaatg tcagtgaacc gacgattgga aatatatccc cctttggtgg
     2221 taccggcact ccaggaaacc tgtacagcat gatactcaac agccagaccc gcttctatat
     2281 tctgaaatct gccagctatg gtaacactct gtgggggaac agcctgaatg atccggctca
     2341 gtgggagttt gttggcatga acaaaaacaa agcagttcag acagtaaaag ataggatcct
     2401 ggccgggcgg gcaaaacaac ccgttatctt tcatggtcag ctgaccggga atatggatgt
     2461 cgccattcca caggtgccgg ggggaagaaa ggtaatcttt gatggtagcg tgaacctgcc
     2521 ggaaggtacc ctgagtcagg acagtggcac cctgatattc cagggacatc cggttatcca
     2581 tgcctccatc agtggcagtg caccggtcag cctgaaccag aaagactggg aaaaccgtca
     2641 gtttacaatg aaaacactgt cgctgaaaga cgctgacttc catctttcac gtaacgcctc
     2701 gctgaacagt gacattaagt cggataacag ccatatcaca ctgggaagtg acagggcatt
     2761 tgtggataaa aatgacggaa caggaaatta tgtcattccg gaggaaggta cctctgtccc
     2821 ggacaccgtg aatgacagga gccagtatga agggaatatt acgctgaacc ataactcagc
     2881 cctggatatc ggcagcaggt tcaccggggg gattgacgct tatgacagtg ccgtcagcat
     2941 cacctctccg gacgtcctgt tgacagcccc gggtgctttt gccggcagtt cactgacagt
     3001 gcatgatggc ggtcatctta cagcactgaa cggtcttttc agcgacgggc atattcaggc
     3061 cggtaagaac ggcaaaatca ccctgagcgg tacaccggtt aaagatacgg ctaatcagta
     3121 tgcccctgct gtatatctga cggacggata tgacctgacc ggcgataacg cagcactgga
     3181 aattacccgt ggagcacatg cttccggtga tattcatgcc tctgcggcat caacagttac
     3241 catcgggtct gacacgccgg cagaactggc ttctgcggaa acggctgcat cggcgtttgc
     3301 cggcagtctt cttgagggct ataacgcagc attcaatggt gccataaccg gtggcagggc
     3361 tgatgtcagt atgcataatg cactgtggac tctgggtggg gactctgcca tccacagtct
     3421 taccgtcaga aacagccgta ttagttctga aggagaccgt acattccgta ccctgacggt
     3481 gaataaactg gatgcaacag gcagtgattt tgttttgcgt acggacctga aaaatgccga
     3541 taaaattaat gtgactgaaa aagccactgg ttcagataac agcctgaacg tcagctttat
     3601 gaataatcct gctcagggac aggccctgaa tattcctctg gtcacggcac cggcgggaac
     3661 ttcagcagag atgtttaagg ccggcacccg ggtgacaggt ttcagtcggg tgaccccaac
     3721 cctgcatgtt gataccagtg gtggcaatac gaagtggata ctggatggtt ttaaagcgga
     3781 ggctgataaa gccgctgccg cgaaggctga cagtttcatg aatgccgggt ataaaaactt
     3841 catgacggaa gttaacaatc tgaacaaacg tatgggtgac ctgcgtgaca caaacggtga
     3901 tgccggtgcc tgggcgcgca tcatgagtgg tgccggttct gcagacggtg gttacagtga
     3961 taattacacc catgttcagg tcggctttga caaaaaacat gaactggacg gtgtggacct
     4021 gtttaccggt gtcacgatga cctataccga cagcagtgca gacagccatg cattcagcgg
     4081 aaagacgaaa tcggtggggg gcggtctgta tgcttcagca ttgtttgagt ccggtgccta
     4141 tatcgatttg attggtaaat atattcacca tgacaatgat tacacaggta actttgctag
     4201 cctgggaacg aaacactaca acacccattc ctggtatgcc ggtgctgaaa cgggttaccg
     4261 ctatcacctg acagaggaca cgttcattga gccgcaggct gaactggttt acggcgccgt
     4321 gtccgggaaa acattccgct ggaaagacgg tgatatggac ctgagcatga agaacaggga
     4381 cttcagtccg ctggttggaa gaacaggggt tgaactgggc aagaccttca gtggtaagga
     4441 ctggagtgtg acggcccgtg ccggaaccag ctggcagttt gacctgctga ataatggaga
     4501 gaccgtactg cgtgatgcgt ccggggagaa acggataaaa ggagagaagg acagccggat
     4561 gctgtttaat gttggtatga atgcgcagat aaaggacaat atgcgctttg gtctggagtt
     4621 tgagaagtca gcctttggta aatataacgt ggataatgcg gtaaacgcga atttccggta
     4681 tatgttctga tgtgggggta aagggctgcc ggaaggcagc ctttctcaga gactcttaaa
     4741 atataaaact gaaggtcttc ggtaaggggg agtgtattag acgtcaacta gttttgccga
     4801 ctggcgcgag cctgtttaag attctgtgta aatgcctttc tcagaagtga ccgtccaggc
     4861 ggtcaccgaa ctcgataata aagcggctca ttgccattcg ccagtccttc aacggcatcg
     4921 tccatttctg ggacgcagac tggattgcca gccacaccac ttttttcacc gagtcgtctg
     4981 tcgggaacac tttacgcttt ttgatcgcat ggcggatcac gctgtttagc gactcgatgg
     5041 cattcgtcgt atagatcact ttgcggatgt ccgttggata agcggtaagc gtacagcgag
     5101 ggccgtattg acggggatgt gttattcagc tggcagtgct atgcgccacg gaagcagttc
     5161 gctgacccgg ttgaccggcc agtctgctat gacgccaagc acatggcgaa ggtagctttc
     5221 tggatccacg tcattcagtt tgcacgtccc gatcaggctg tacagtagcg cgccccgctc
     5281 accaccatgg tcagagccga agaacaggaa gtttttacga cccagactga ccgcccgcag
     5341 ggcattttca gcgatgttgt tgtcgatttc cacccagcca ttactgcagt acacgttcag
     5401 ggccttccac tgtttcagta ggtatgcgaa cgcttttgcc gtatccgagt gacgcgacag
     5461 tgttttcatc tgttgctgta tccggtcata cagtgactgc atcagtggcg cggctcttgc
     5521 ttttcttgcc gccagacgct gttctgctga acatccccgg acttctgcct cgatggtata
     5581 cagttcaccg atacgctgca gggcttccgt ggtgatgtcg gttggcactc ttgcatgcac
     5641 atcgtggatt tttctccggg catgggccat acacgcagct tccgttattc tgccggattc
     5701 gtataacgcc cggtaaccac cgtaagcatc tgcctgaagc acaccgctgt aaccggctag
     5761 gtgattctgt ggatggatac ctttccggtc cgggctgtac gcgaaccaga ccgccggggg
     5821 catctgtgaa ccggcgttac ggtcatcacg gacatagacc cacagccggg cagttcttgt
     5881 tttaccactg cccggctcct ggaccggaac ggggatatca tccgcatgaa ctttaccggg
     5941 catcagtaca tactggcgca ggacgtcata cagcggctcc agcagttcag caacggcacc
     6001 ggtccagcga cccagtgtgg cacggctcag ctccacgccc tgacgacggt atatttctga
     6061 ctggcggtat aactgtcaac gacggatgaa aagtggctct tccgcaacct gggtggagat
     6121 tttcatgcca gtggactcat taccctctgc ctgagtagtt caaatcctgc tcgaccatac
     6181 atctggcgtt tcagcatctt cttcaggcga tttacatgac cttcgacaac accattactc
     6241 cagcgactac tgattgcctc acatatagct gcagcatcag cttccatccc cgccgccacg
     6301 cgccgaaatt ctgctgaacc gctttcgtgg actcgagtga accaactgct tagctgtgat
     6361 ttattttggg ttttcagaat acggtagaac tcgagtgcca gttgctgcgc tattttcagc
     6421 tcaggttctt tttcacacat cagactaata aatcgggaag cataattttc ttcatccctg
     6481 gttattctcc agggcatcaa ccagcggctt acccgggaga ctgaaggaag tctgacggca
     6541 gtagtaaccg gtgggttcca gcctttacgc catttggcaa ccgcatccct gacgatggtt
     6601 tcactgcctg taaatccccg ggccaccatt tcccgccata tccggctggc gttataatta
     6661 ccactttctc gttgctcttt taaccactcc ctccatggtg tcagcgaaaa acgaaaccgc
     6721 tgttgaaatc cctggaaagc tggttgcgga aaagatgaag acccagtcgc gacactcaga
     6781 gttggcgaag gcgttcgcgt acgcacttaa ccagtggccg gcactgacgt actatgcgaa
     6841 cgatggctgg gtggaaatcg acaacaacat cgctgaaaat gccttgcggg cggtcagtct
     6901 gggtcgtaaa aacttcctgt tcttcggctc tgaccatggt ggtgagcggg gagcgctact
     6961 gtacagcctg atcgggacgt gcaaactgaa tgacgtggat ccagaaagct accttcgcca
     7021 tgtgcttggc gtcatagcag actggccggt caaccgggtc agcgaactgc ttccgtggcg
     7081 catagcactg ccagctgaat aacacatccc cgtcaatacg gccctcgctg tacgcttact
     7141 catcacctgc tattaatccc cgagtcagta cttcgcggcc tgacgcgatc accctactca
     7201 gtacttcaga gcccgcctcc ggtacctgcc tggcgatcgc ccctgtgcaa caaaataaat
     7261 caaccgttat caaaccacga tcgttcgtat gcgccgccga tgacgcgcgc ctgaaaaacg
     7321 cgtcaatatg agttccccgg aagcttacga taatacgctt atgaacatta caacacgctc
     7381 atgcggaacc tggatggggg aaggaacggt gaagctgtta gcgaatgacc ggcgtttata
     7441 caaacgccgg tcatgatgaa tgatatttca gaatatacct cgggctaaaa taccgggaca
     7501 gagatcaggc gtgattaatc tgcagcattt ttaataacag aagtactgag cttactttac
     7561 ctttgatgat gtaatagtta tttccgtcgg tactcatttt tccctgaaat aatgtcacgg
     7621 atgtttcttc ctgctgatcc tgcaggcgca gtcgtaaggt catattttta atgagctgag
     7681 atccgggtgg caaggggtat ttgctgtcag atactgggcg actgagtctg atgtggcgcg
     7741 tatacagccc gttttcgtac agggtggcct ctgttatggt gtcagtgacc agcatgtggg
     7801 agacaatgct gtattctttt ttaaagcacc caccagaaac ggtgattgta atgataatgg
     7861 tcttctcgat cgagatgtaa atacccgttt cgttatactg tccgtatcgg aaccatgaga
     7921 actcagttgc attccagcca tgctccagaa tatgtgcatt tttttgcctg atagtttctt
     7981 tttttattcg ctcatgttta aggcataaca ggcagataaa taatgtcaga aaaagaaagc
     8041 taataatgag agcaagagcg atatagtctg tcacagggta atacatattc tctccggtag
     8101 aagaagggcc gcaaacgcgg cccgggctgt tatcagaaag agtaacggaa gttggcgttg
     8161 atcgcgttat ccacgttgta tttaccaaat gccgatttct caaactcaag accgaagcga
     8221 agattatcgc gaatttcggc gttgagacca acattcatga gaatacgacc gtctttttca
     8281 cctttgatac gtttctcacc ggacgcatca cgcagtacgg tttcaccgtt ggcaaacagg
     8341 tcaaactggt agccaaggcc ggcgcgggct gtgactttcc agtccttacc ggagaaggat
     8401 ttacccacat caacaccggt acgcccaatc agcggattaa agtccttatc cttcatggtc
     8461 acgctcattc cgcgatcctg ccagtcgaac cgtttacctg atacggcccc ataaaccagt
     8521 tctgcctgcg gctcaatcca ggagtcttct gttacatgat agcggtagcc tgcttcagca
     8581 cccacataca aggaatgaga actgtagtct tttgttccga gtccagcaaa ggtcgccgag
     8641 tactcattat catggtgaac atacttacta atcaggtcga tataggcacc agagtcaaat
     8701 atagcggaag catacagacc tgccccgacg gacttcgtct tgccactgaa tgcattactg
     8761 ctggcatgac tgtcggtata cgtcatagtc agaccagtga aaaggtcaag tccatccagc
     8821 tcatgttttt tatccacacc aatctgcaca tgggtgtagt tgtcactgta tccaccagaa
     8881 gctgaacctg ctccgctcat gatgcgggcc catgcaccgg cctcgccatt ggtgtcacgc
     8941 agatccccca ttcgtttatt caggttgttg acttctgtca ggaaggcttt gtaattaccg
     9001 gacatatacg attttgctgc accggaagat gctgtatttt cagccacctt atatccggtc
     9061 agggtccacg tggtatgttc ctgatcctct ttcgtgctga tcacaggaga tatgttgctg
     9121 aaacctatgg cctgagtact ggctttaaat atctctttat tgcttccttt tggggctgtt
     9181 atcaggtcaa cgtttaatcc tgagttatta tttcccactt tctgtatatc gaccgacaga
     9241 ctgttattgc ttccggttaa ctgattttta acaatgagtt tatccgattg atttacagac
     9301 tggctcagat cagcccgcat aatcacagca gtattagtgg catccagtgt gtttactgtc
     9361 acagtatgga aacccgaatt ctctatgtta ctaaacaaca aaagactgtc gcgggttttt
     9421 aatgattgca gggctgactg tccgtttact ctccataagg tgttatacat gctggctgag
     9481 gctgcaggag catcaatacc tccggtataa gaagtatcaa agccacccag caaacctgta
     9541 gccagcggtg tgtgtgtggt tttgtccagg ttatcttctt tatactggtc aacaccaaac
     9601 agaatagatg cttttttgtc tgattttata ttaccattga cccaggcata attttgagcc
     9661 tttaacgtgg caccatcttc tgtcatatta tatccatctg acatatagat tgccgggctg
     9721 tatttattat tttctgtagg ggtgcctgtt aatgtcaggg ttccggccag ttcaatagct
     9781 ttatctacat acatttttcc ctgggcggta acagtagctc ctttgttgat aatcaggtct
     9841 gtattaatta aggataatgc accagtcata gtaacagttg atttatcacc aacagttatt
     9901 ttgctgtttt gagcactcag actgccgttg aaactacttt cgcctgctga atccccggag
     9961 cgaagctgtt gtctgaatcc aaaaccttcg ccagtaatgt ttcttcctga gaacatgtca
    10021 atgtatgcag ttttatttcc cagaacaatt tgagagtttt tagcctctat gtgtcctttt
    10081 acattagcat cccggcctat actcagggtt gcgttttcta aatttaattt ccggaaatta
    10141 aatttacgac tttcccagtc gggctgggaa aaagaggcta tctgattgtt ggacttatat
    10201 tccgtattgt aacgtttatt tacagtgctt tcctggtcct ttatggcagc agaatagtct
    10261 ttttgacata aaaaaggaat tggacagttg ttattgcctt ctttaaatat ggcatgttct
    10321 gtcgcatgtc cttgtaatgt taactgtgca ttccggacag agatatcgtt tttgatatcg
    10381 actgagccat caaagatcag attggcatta ttgttatgtt gctgggtagt attgataata
    10441 tgatttatgc ttatattacc tgatacattc ccatggtaca tatatgcatc agtattagta
    10501 agtgataatg ttgattgctt cacgtttgag ttcgttacgg ttgttcccgc atctgttgct
    10561 gcgattttct gaaatgactg gtcatagccg tttagatcca gtgttccgcc atttctggta
    10621 aaatagattt cgccattacc gctttcgctt aaagcgtctt tggcatttat ttttaccgta
    10681 cctttacctc cggccatgta aattttattg aaggcttttt cagcactaag gatgacagtc
    10741 ccattaccta ttttaaggtt atttccctgt gctattttta catccagagt accactacct
    10801 attttatgca gattatctcc ggcaacgccc ttaacattcc attcaacggt ggtattatta
    10861 tcaatatcta tcccggcacc tttataagac ttatcttttc cgtacacttt atatgtctgg
    10921 ttcttatcga atataagccc gccaatgcca agatcggtat cctgcttgag cgttaatacc
    10981 cctcctcctg agacagacag gtccttatct tttataatcg atgataactc ttgtttttta
    11041 ccatccagct gcatagtgtt tgtgaaattc ttttttaaat tgtttaccag aatattatta
    11101 aataacgagt agtttgtcca ggctatattg gtactactga taaaaccaac tgtactggta
    11161 acaccatgga aaacccattt ttgttgttct ttatcatata gataagaacc agaaccacta
    11221 tcgccaaaaa ttggtgagtt tttaaaaggg gttctgttgg tataaatgta actcttgttt
    11281 ttccagtcca gattaaaaag acttgcgctt aacaagtcgg gattatagtt ttgtccaaac
    11341 agatattgtt tgccgtttaa tatcgttgag gttgttccgg cacctgctct gaagccaatt
    11401 atttgttcct taccgttgta gtttacgcca tatcttttca tcgcatcttc gggagaaagt
    11461 gagaagtcgg cactctccgt aacccctgtt gtttccacga cgaatttatt cagacgatga
    11521 accgaaaagt cgccagatga tactctgtta cttgctttat atgctgtctg atcccatgac
    11581 tgtgttgtta ttgcatgatg ctgtgtaccg ttatgagtcg cagtaacact gtacgcacct
    11641 ccaattgatg ttgttgcacc tttgttggaa acagcagaaa aatcaggtac aggtagttct
    11701 ggaaaatgaa atatctttcc atctttcatc attaattgaa catttgttgc acccggtctg
    11761 aactctcctc tgttttgagc aaggtcaaga taatcgcggg tccatatatt atttatatcc
    11821 agcattgctg caaatgctgt ttcgccataa cttccatata ttgaggataa aataataccg
    11881 aggataactt tctttcttga tgtaccgaca ctaactttcc gggtcagttc agaaacagca
    11941 actaatccac ctgtaatatg actatatttc agtgaataaa ttttattcat acgtgaactc
    12001 cgatatttct gagttgtggt tatgggatag catttaatta caacggggaa ctatcagtca
    12061 tggtacgtaa ctgataaggg tataatgata atcactgtca gttatatttg tatgacagat
    12121 gtaacaaaat gaaaaaaaag ataagcacaa gtgattgatt ggttagatat tgatcacttg
    12181 gtgtttgtat tttgaatttt gcgatcaaaa tttgatcttt gttgtttccg gtcaatcctt
    12241 tacaggtttt ctgtattatc cgggggagtt aagtctgtcg atgtccgaca gacttttgat
    12301 gatggaaaca acatgcagcg ataatgtgca tgctgttttg caggtggaac tcagaaggtg
    12361 aactggaact gtgcgaccgc accgtaagtg ttgtccgtgc cgatcaggcc tgcaacatca
    12421 aggtgaacca catcaaacgg tgagatcccc accccggccg tgaatgcact gccgtcgtta
    12481 ccggccagat tctgacggta accggcgcgc agctgtgccc atttccaggc attaaattct
    12541 gtgccaatgg cggcaaactg acggttgctg tcggaggtga aaccgctggc cggggtcaga
    12601 tccacatcaa atgcagtggt gaacatcgcg ttgtgccagg agacaccggc cgtcacctgc
    12661 gggcggatcc tgaaggtttc cgtgataccg ttcacttctt ttgtctcaat actacgggag
    12721 atcaggttct gtgcgaccag tcccagcgtc cagttgtcat ccaggtccgt actggcaccg
    12781 atgtcggcgt tgatgccatt tttcgtgttg tggtagcggt cgcctttgaa ggcgctgctg
    12841 tcataatttc tgaccagcac gttgtagttg aacaggtcga cgcgctggaa tttggggggg
    12901 aagcccagag accatttctg cccggccgtt tccagttccg tagccagagc aatacccaca
    12961 tcagtgatca gtgccgtgcg ggcacaggcc tgtgatgtca ggcggctttt gtccacctcc
    13021 tgcccggtgc tgtttgccac ctcttccagg taagtgaggt cagcatcaga gactttaccg
    13081 tcgacactga cggtgccgtg tgatttaacc atcagtgcgg cagagaggcg atcaccggga
    13141 agggccgcca cagcagaaac gcccagctgt gcggcggcat gtgtgttacg gaagtcccgg
    13201 aggtgttctt tcaggcgtgc ggcagcctca ggaacaccgt ggttactttc gatggcgcgg
    13261 tcgaaggcct tccactcatc actgatccgg tcggcgttgt cggtgatatt atccggattg
    13321 gagagctgcg cgccgacgga cggaagcagg atgctgggct catgcgcgac gcaaaatcca
    13381 cgatgtatat atcagtacta aaagcgcgac ggcggaagaa gctctgaaac taatcggtga
    13441 actgtacgcc atagagcacg atatacgcgg gttaccggtg tctgaacgcc tggcggtcag
    13501 gcaaatgcag agtagaccgc tcctgacttc cctgtataag ctgatgcagg agaaagaaca
    13561 cacgttatcg aagaaatgtc gtctgggaga tgcgttccgg tatatcagga agcactgggc
    13621 tgcgttgtgc aacttctgtg atgatggcct ggctgaggta gataataatg cagcagaaag
    13681 agcgcttcgt gcagtctgtc tcgggaagaa aactttttcg aggatcactc attccgtgtc
    13741 tgactgcttc agggtttaat tttgcttctg ctgactgaat gatgattggg cagcaagtgc
    13801 aatatcccac ataaatccag tcaactcacg agccaccgcc gtaatggcta cttgtgattt
    13861 tttccctttt gctgtcagag ttcggtaacg agaacacaat cgttgttgag ctttccatgc
    13921 aatatctttg gaatattgtg ttacagagtc cggtcggtaa tatataagcc atgctccaac
    13981 ttttgcaggt gtacgataag accaggcggc ttcgtaaagc agacgtctca gttcgctgtt
    14041 tccaactttt gttattcctc tgggacgaat actgtttcca ctggaatatt cacctggcac
    14101 ca
//
LOCUS       DAZPCS010000097        13964 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZPCS010000097 DAZPCS010000000
VERSION     DAZPCS010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13964
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.097"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            191..2038
                     /gene="ldtD"
                     /locus_tag="ACHUQ7_002733"
     CDS             191..2038
                     /gene="ldtD"
                     /locus_tag="ACHUQ7_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR1136071.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPIKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLVLLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLLYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWEVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADDRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2219..2767
                     /locus_tag="ACHUQ7_002734"
     CDS             2219..2767
                     /locus_tag="ACHUQ7_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFR1136072.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2794..3441
                     /gene="gloC"
                     /locus_tag="ACHUQ7_002735"
     CDS             2794..3441
                     /gene="gloC"
                     /locus_tag="ACHUQ7_002735"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFR1136073.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="ACHUQ7_002736"
     CDS             complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="ACHUQ7_002736"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFR1136074.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGILEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSSDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="ACHUQ7_002737"
     CDS             complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="ACHUQ7_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFR1136075.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKTVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="ACHUQ7_002738"
     CDS             complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="ACHUQ7_002738"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFR1136076.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8297..9499)
                     /gene="pncB"
                     /locus_tag="ACHUQ7_002739"
     CDS             complement(8297..9499)
                     /gene="pncB"
                     /locus_tag="ACHUQ7_002739"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFR1136077.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9765..12380
                     /gene="pepN"
                     /locus_tag="ACHUQ7_002740"
     CDS             9765..12380
                     /gene="pepN"
                     /locus_tag="ACHUQ7_002740"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFR1136078.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEEGALVISNLPERFTLKIINEISLA
                     ANTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNG
                     NRVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDR
                     GNLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKY
                     VLARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSD
                     LGSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMI
                     HTLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVT
                     VKDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVN
                     SVLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNGFSRW
                     DAAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVN
                     EMAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLR
                     NACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKW
                     HQDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAF
                     HAEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLE
                     NLSGDLYEKITKALA"
     gene            complement(12587..13354)
                     /gene="ssuB"
                     /locus_tag="ACHUQ7_002741"
     CDS             complement(12587..13354)
                     /gene="ssuB"
                     /locus_tag="ACHUQ7_002741"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFR1136079.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLHLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAAGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKISLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(13351..>13964)
                     /gene="ssuC"
                     /locus_tag="ACHUQ7_002742"
     CDS             complement(13351..>13964)
                     /gene="ssuC"
                     /locus_tag="ACHUQ7_002742"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFR1136080.1"
                     /translation="HLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQML
                     RNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARSYG
                     LSGIPLFIHVILPDALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREFLQ
                     TDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATI"
BASE COUNT         3550 a         3563 c         3527 g         3324 t
ORIGIN      
        1 taacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgctgg tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgtgac gtggtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccgatcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcgcttt caacgccgcc gctttcggtg attaaccaat ggcagctagc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggtctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gtgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggagcaga
     1201 tggtgctatt ggcccggcaa cgcgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggca ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccagcct attcgctgct ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcacttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt accaccaact ctggcacgca aagatattct
     1501 gccgaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg ggaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg tttcgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggatg gaatgacaaa cgtatttctg atgcgctgaa
     1861 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct
     1921 gacggccttt gttggtgcag atgatcgtac ccagtatcgt acagatattt acaattatga
     1981 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
     2041 gaagtagttc ggggaaatca attgtcgtaa taattagcat gattgggggc gattctctgc
     2101 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
     2161 cctcttgtgc gccagaagtg catataaacg ataacattga gctgtagact tgattatcat
     2221 ggacaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc
     2281 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
     2341 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
     2401 tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
     2461 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac
     2521 gcgcaaaccg gtgcaactca tttccggtta tcgttccatt gataccaaca atgaactacg
     2581 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
     2641 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
     2701 tggtgtagga tattacccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
     2761 ctggtagtaa tcgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
     2821 gcattctccc agaactgttc attgatctgg tgtgaacaaa cccgcctggc cgcactggtc
     2881 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg
     2941 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
     3001 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
     3061 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agccgctgac gccagatcgt
     3121 tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc
     3181 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
     3241 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
     3301 caactgattt cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt
     3361 ccgggtcacg gaccattatc tacacttggt tatgagcgcc tgcacaatcc cttcctgcaa
     3421 gacgaaatgc ccgtctggta aaggcgcata aaaaagtcca cttttaatgc gggcctggat
     3481 ttctggcaaa gtgcgctttg tttatgccgg atgcggcacg agcgccttat ccggcctaca
     3541 aaatcgtgca aattcaaaat attgcagggg acgcgtaggc ctgataagcg tagcgcatca
     3601 ggcaatgttg cgtttgtcat cagtctcagc ccgcttttca gcgggcttca ttgtttttaa
     3661 cgcttacagc actgccacaa tcgattcgca cagcggagcc atgttatctg gtgtcatccc
     3721 ggccacattt acgcgaccag aagcaacagc atatacgcca aactcttcgc gcagacgcag
     3781 cacttgttct tttgtcagac cactgaagga gaacatgccg ttctgtttga tgataaagct
     3841 gaagtcgcgg tttgcacctt tttcctgcag cgtattgacg aacaactgac gcatacgctg
     3901 aatacgctgg cgcatatcag tcagctcttg ttcccaaatc gcacgtaatg catcgctgct
     3961 caggatggtg gcaacaacag aagcgccgtg tgctggaggg ttagagtagt tagcgcgaat
     4021 cgccgctttc atttggctga atgcgcgatc aacgatttca ctgtcggcag caaccagagt
     4081 acaagcgcca acacgctcgt tgtacaggcc aaagttttta gagtaggaac tggcaacaat
     4141 cagctcttta tgcatagccg cgaaagcgcg cagtccttca gcatcttctt ccagaccacg
     4201 ggcaaaaccc tggtaagcga agtcaaacag cggtaaccag cctttctcaa cggagagttg
     4261 tgccagtgtc tgccattgtt ccagcgtagg gtcgataccg gttgggttat ggcagcagcc
     4321 atggaacagc actacgtcgc cagcctgagc ttcgttcaga ctgttaatca gtgcatcgaa
     4381 gtcaagggtg tgattttccg catcataata agcgtattca cgaacttcca gacctgcaga
     4441 gttaaagacg ctcttatggt tcggccagct tgggttgctc acccacacac gcttaacgct
     4501 ggtatttttt gccaggaagt cggcagccac gcgtagtgcg ccagtgccac ccggagtctg
     4561 tgccgtgcga gcacgtttgt cattgatcag ggcgctacct ttaccaaaca gcagttcctg
     4621 agtgcagcga ccaaattcaa ggatgccgtc aatgccgagg taatttttgg tggtttcatt
     4681 ttcgagcaga tactgttcag cctttttcac gctggtcagt accggggttt tgcccgtctc
     4741 atctttatag acaccaatcc cgaggttaat tttgccggga cgttcatcgg cacgaaacag
     4801 atcggccagg cccagaatcg ggtcggcagg agcggcggta atgttctcaa acatgacgag
     4861 gttccattat ggttacagaa gggaagtccg ctatcagggt aacgggagat ttacaaaatt
     4921 ccaactatta ctgatgaaaa cgcaggctgt ttttgcaaga cgtgagattg ctctggaagg
     4981 cataaaaaaa acaggaccaa cgtcctgttt tttcggcatt taacaaagag gtgtgctatt
     5041 agaactggta aacgataccc acagcaacgg tgtcgtctga acctacgccc agtttgttgt
     5101 cagaatcgat ctggttgatg atgtagtcaa cataggtgga catgtttttg ttgaagtagt
     5161 aggttgcgcc cacttcaaag tagttcacca gatcaacatc accgatacct tctacgtctt
     5221 tcgctttaga tttggtgtaa gcgatggacg gacgcagacc gaaatcgaac tggtattgcg
     5281 caactaacag aacgtcttgc gttttgttgg cgaagccgct ggtgtttgta aatttattag
     5341 tgatcggcgt agcgttacgg gtttcaccgt agttcgctgc caggtagatg ttgttcgcgt
     5401 cgtacttcag accagtagcc cactgttcag cttttttacc gttgccaaga ggttgagctt
     5461 cttgcaggtt ggtacggtca gctgcaccat aagcaccaac gataccaaag ccttcgtatt
     5521 cgtagctgat agaaccgcca acaccgtcgc cgttagaacg gcgtgcagtg tcacgctcgt
     5581 ttttacccag gtactgaaca gcgaagttca ggccatcaac cagaccaaag aagttggagt
     5641 tacgataggt agcaacgccg ccaacacgac caacgaagaa gtcatcgctg tatgcagtat
     5701 caccaccaaa ttctggcagc atatcggtgt aacccagtgc atcataaacc acaccgtagt
     5761 tacggccgta atcgaaagaa ccaacgtcag cgtatttaag acccgcgaat gccagacgcg
     5821 ttttgttacc agtttgagcg tcagcgcctt cagagttgtt accctggaag ttatattccc
     5881 actgaccata accggtcaga tcggaattga tttgagtttc ccctttaaaa ccaagacggg
     5941 cataggtcat gtcgccattg ccaccgtaac tgttttcacc gttacccttg gaaaaataat
     6001 gcagaccaac agttttaccg tacagatcta ctttgttgcc atctttgtta tagatttctg
     6061 cagcgtttgc agtacctgct actaacagag cagggacgat cactgccaga atattgcgct
     6121 tcatcattat ttattaccct catggttttt ttatgacacc tgccactgcc gtcaataagt
     6181 tctgtcaata aaaatttacg gaactattga tgagagtttg gtgtctttat gtgtctgcag
     6241 gcatctttcc attcaaacta acgtttcgct accgtgaaag tgctacaaag ataaagattt
     6301 agtttcaaaa agaaaaaata tgtaaccaaa agtaaaaatt aaggagcttt gtgaacaccg
     6361 tcatatttcc ataggaacgt gatgatattt acagcaattt ttatctattt atatgattta
     6421 cttatattta aattaactaa acggaaattt tgtttctgat ggaaacttta tcgacctggc
     6481 acaaaatcct ctttcagaca tccagaatgc caaaaaatag tatgaaaatt gtgctatcag
     6541 ctaagaaaaa actaatccgc aataagagta cgggttattt tttgtgcgta aatgttgcaa
     6601 tgtaatattc cgtaacagga tgatcgttat ggcgggggtg caaatagatt gcttgactta
     6661 acaaacagat aacttgacag aaaggataaa aaagagccag caggcgctgg ctctttgagg
     6721 aagaaaatca gaagctggcg ttgcgcggag tacgtgggaa cggaatcaca tcacgcacgt
     6781 tttgcacgcc agttacgtaa gcaatcaggc gttcaaaacc aagaccgaaa cctgaatgcg
     6841 gaacagtacc gtagcgacgc agatcgcgat accaccagta atcttcttta ttcaggccca
     6901 tttccagcat acgctcgtcc agaacgtcca gacgttcttc acgctgggag ccaccaatga
     6961 tctcaccgat gcccggagcc agaacgtcca tagccgcaac ggttttaccg tcttcgttga
     7021 ggcgcatata gaacgcttta atatctttcg gatagttttt aaccactacc ggtgctttaa
     7081 agtgttcttc cgccagataa cgctcatgct cagaagagag atcgacgccc cagtaaaccg
     7141 ggttttcaaa cttcctgccg cagttttcga gaatggtcac tgcgtcggtg taatccacct
     7201 gcgcgaaatc ggcttcaatg aagcgttcca gacgtgaaac ggcatcttta tctacgcgtt
     7261 cagcgaagaa tttcatgtcg tctgcgcgtt cttcgagaac cgctttgaag acatatttca
     7321 gcatggcttc agccagaccc gcgatatcgt tcaggttagc aaacgccact tccggctcca
     7381 gcatccagaa ttccgccagg tgacggctgg tgttggagtt ttcagcacgg aaagtcgggc
     7441 cgaaggtata aattttggat aatgcgcaag cgtaggtttc gccgttcaac tggccagata
     7501 cggtcaggaa agactcttta ccaaagaagt ctttgtcgaa atccactttg ccctgatcgt
     7561 tacgcggcag gttttccaga tccagcgtag aaacgcggaa catttcgcct gcaccttcgg
     7621 tatcagatgc ggtaatcagt ggcgttgaaa cccagaagaa tccctgctcg ttaaagaagc
     7681 gatgcagcgc ctgcgccagc gtatggcgaa cgcgcgcgac ggcaccaatc aggtttgtgc
     7741 gcggacgcag gtgagcgact tcacgcagat actcaatgct gtggcgtttt gccgccatcg
     7801 ggtaagtgtc tggatcttca acccaaccag caacttcaac cttgctggcc tgaatttcaa
     7861 attgttgccc ctggcccggc gatgccacga ctttacccgt cacaatgacc gagcagccgg
     7921 tggtcagacg caggacgtct tcattgtaat tgggcagaga attattgatg acagcctgta
     7981 caggatcaaa gcaggaaccg tcataaacgg cgaggaagga gatgccagct tttgaatctc
     8041 ggcgggtacg tacccatccg cgtacggtga cttcgctgtc aacggctacg cggccctgga
     8101 gtacgtcggc tacaggcaca acgctcataa tattctctct gttaatagtc ggaaaaaata
     8161 aacacttgtc cacccgtaaa tgggggggat acctatgtta cctggcatct gcaatcagac
     8221 aagcagaatt cgcaaatgca gcgaaagatt tcggaattaa aggtaaaaaa gggagccgat
     8281 tagctccctg atgatattaa ctggcttttt taatatgcgg aaggtcgaac gctttgcgca
     8341 gcgcccgaac aaacgcttta tcatggcaga tagttttgcc agggctgtca gaaagtttcg
     8401 ccaccggttt accgttacac tctaccaact taatcacaat attcaggggt tttacctggg
     8461 ggatatcgca ggtcaggcga gtcccaatac caaaacttaa ttgcacgcgg gaagagaagt
     8521 ggcggtatag ctcaaccgct ttgcgtaaat ccagattgtc agagaaaacc agcgttttac
     8581 tctgtggatc aattcccagc ttttcataat gtgcaatggc tttttcaccc cattcaaccg
     8641 ggtcgccaga gtcatgacgc aggccctgat accgactagc gaactcgaca ccgaaatcac
     8701 gcaggaaagc atccatagtg atgcagtcgg ttaatgcaat gccaagttgg tcgggatact
     8761 cttccagcca ggcagcaagt gcagctcgct ggctgttggc tagatccggg ctgatttgct
     8821 gatgtgcctg gaaccattcg tgtgcctgtg ttcccatcgg cgtgagggaa agccgacgcg
     8881 ccagatcgta gttgctggtg cccacgaacc aggattcctg ttgcagacgc ttaacgatgg
     8941 tttcttgtac ttcgcgagaa aaacggcgac gggtgccaaa atccatcaga tggaagcgcg
     9001 acatatcaag accggcggtt aacgccgaga agtcgactaa tttgctttcc agcgtgtcga
     9061 gggcttgcgc aacgtcggcc tgcggtgagc gatagcgatg taccatttca ctgataaccg
     9121 ccagcaaagg aacttcccag aggatgactt cacgccacgg accgcttaag cgaatatcca
     9181 gcttgccatt atcgttggac acggtgactt gttccgggtt aaagcggaac tcgcgtaacc
     9241 agttaagata gtcggcctta aagaaaggca gggcagagag ccactgatat tcatcatcct
     9301 gcaggcgcag gtgctgcatc gcctgaatct gttcacgaat agcatcggca taaataccca
     9361 gcagatcgtc acctcggcaa cgaaactccg ccgcgacatg cacatcgtaa tagtgatgaa
     9421 acacggcttg ctgcatatgc aacttataag catctgtatc cagcaacgag tgcagaacag
     9481 gagaagcgaa ttgtgtcata ggtgcgcagt agcgtcctcg tacaggagcg tttagtacaa
     9541 taaacatctt caggaactgc tggagtatac cttgtttcgc aatttattga accccgatca
     9601 caccatatgc cacctttctg gtcgatggca ttacgcggtg tatgttataa aaatgtagca
     9661 ataaaggcgt ttgtacctga aaagatgaag attctgcata gcgcgattta cgcaacagga
     9721 atagactgaa caccagactc tataaaagat gctaaaggtt atttatgact caacagccac
     9781 aagccaaata ccgtcacgat tatcgtgcgc cggattacca gattactgat attgacttga
     9841 cctttgacct cgacgcgcaa aagacggtcg ttaccgcggt cagccaggct gtccgtcatg
     9901 gtgcatcaga tgctccgctt cgtctcaacg gcgaagacct caaactggtt tctgttcata
     9961 ttaatgatga gccgtggacc gcctggaaag aagaagaaga gggcgcactg gtcatcagta
    10021 atttgccgga gcgttttacg cttaagatca ttaatgaaat aagcctggcg gcgaatacgg
    10081 cgctggaagg gctttatcag tcaggcgatg cgctttgcac ccagtgtgaa gccgaaggtt
    10141 tccgccatat tacgtattat ctcgaccgcc cggacgtgct ggcgcgtttt accaccaaaa
    10201 ttattgccga taaaatcaaa tatcccttcc tgctttccaa tggtaaccgc gttgcgcaag
    10261 gcgaactcga aaacggacgc cattgggtac agtggcagga cccgttcccg aaaccgtgct
    10321 acctgtttgc gctggtggca ggcgactttg atgtactgcg cgataccttt accacgcgtt
    10381 ctggtcgcga agtagcactg gagctgtacg tcgatcgcgg caaccttgat cgcgcgccgt
    10441 gggcgatgac ctcgctgaaa aactccatga aatgggatga agaacgcttt ggcctggagt
    10501 atgacctcga catctatatg atcgtcgcgg tggatttctt caatatgggc gcaatggaga
    10561 ataagggtct gaatatcttt aactccaaat atgtgctggc ccgcaccgac accgccaccg
    10621 acaaagatta cctcgatatt gaacgcgtta tcggccatga atatttccat aactggaccg
    10681 gtaaccgagt gacctgccgc gactggttcc agctcagcct gaaagaaggt ttaaccgtct
    10741 tccgcgatca ggaattcagc tctgaccttg gttcccgcgc agttaaccgc atcaataatg
    10801 tgcgcaccat gcgcggattg cagtttgcgg aagacgccag cccgatggcg cacccaatcc
    10861 gcccggacat ggtcattgag atgaacaact tctacaccct gaccgtttac gagaagggcg
    10921 cggaagtgat ccgtatgatc cacaccctgc tgggcgaaga aaacttccag aaagggatgc
    10981 aactctattt cgagcgtcat gatggtagcg cagcgacctg tgacgacttt gtgcaggcga
    11041 tggaagatgc gtcgaatgtt gatctctccc atttccgccg ttggtacagc cagtccggta
    11101 cgccggttgt gaccgtcaaa gacgactaca atccggaaac tgagcagtac accctgacca
    11161 tcagccagcg cacgccagcc acgccggatc aggcagaaaa acagccgctg catattccgt
    11221 ttgccatcga attgtatgac aacgaaggta aagtgatccc gttgcagaaa ggcggtcatc
    11281 cggtgaattc cgtgctgaac gtcacccagg cggaacagac ctttgttttt gataatgtct
    11341 acttccagcc ggtgcctgcg ctgctgtgcg aattctctgc gccagtgaaa ctggaatata
    11401 agtggagcga tcagcaactg accttcctga tgcgtcatgc gcgtaatggt ttctcccgtt
    11461 gggatgcggc gcaaagtttg ctggcaacct acatcaagct gaacgtcgcc cgtcatcagc
    11521 aagggcagcc gctgtctctg ccggtacatg tggctgacgc tttccgcgcg gtgctgctcg
    11581 atgagaagat tgatccggcg ctggcggcag aaatcctgac gctgccttct gtcaatgaaa
    11641 tggctgaact gttcgatatc atagacccga ttgctattgc cgaagtacgc gaagcactca
    11701 ctcgtactct ggcgactgaa ctggcggatg agctgctggc tatttacaac gcgaattacc
    11761 agagcgagta ccgtgttgag catgaagata ttgcaaaacg cactctgcgt aatgcctgcc
    11821 tgcgcttcct tgcttttggt gaaacgcatc tggctgacgt gctggtgagc aagcaatacc
    11881 atgaagcaaa caatatgacc gatgcgctgg cagcgctttc tgcggcggtt gctgcacagc
    11941 tgccttgccg tgacgcgctg atgcaggagt acgacgataa gtggcatcag gacggtctgg
    12001 tgatggataa atggtttatc ctgcaagcca ccagcccggc ggcgaatgtg ctggagacgg
    12061 tgcgtggtct gttgcagcat cgctcattta ccatgagcaa cccgaaccgt attcgttcgt
    12121 tgattggtgc gtttgcgggc agcaacccgg cagcgttcca tgccgaagat ggcagcggtt
    12181 accagttcct ggtggaaatg cttaccgacc tcaacagccg taacccgcag gtagcttcac
    12241 gtctgattga accgctgatt cgcctgaaac gttatgatgc caaacgtcag gagaaaatgc
    12301 gcgcggcgct ggaacagttg aaagggctgg aaaatctctc tggcgatctt tacgagaaga
    12361 taactaaagc actggcttga tacttttgag ccaaaatgcc tgatgcgacg cttgacgcgt
    12421 cttatcaggc ctacaactcc gttgaaattg ctggttttgt aggctggata aggcgttcac
    12481 gccgcatccg gcagccgtgc ccaactgcct gatgcgatgc ttgacgtgtc ttatcaggcc
    12541 tacaagtccg ttgaaaatgc tggttttgtg cgccacatcc ggcatattaa ccatgacgcc
    12601 gaatcagttg ttcagaatgc ccacgccgca ttacccgttg caatacttcc gcttccagct
    12661 ccgctaatcg caccgaccct aatcgacgcg gacgggggat atccactgtc aaatccagac
    12721 taatttttcc ctcttcaatt aacaacaccc ggtcagccat tgctaccgct tcgctcacat
    12781 catgcgtcac caacagtacg gtaaagccgt gctgctgcca aagtgacaca atcaaatcct
    12841 gcatctcgag tcgcgttaag gcgtccagtg ccccgagcgg ttcatcaagc aacaataaac
    12901 ccggtcgatg aatcaacgct cttgccagcg ccacacgctg tttctgcccg ccagaaagtg
    12961 cggcaggcca ttccccggcg cgattctcca gtcctgccgc agccaacgcc cgacgcgcgg
    13021 catcgcgcca ctgaccttta aggcctaacc caacgttatc aatcaccgat ttccacggca
    13081 gcagacgcgc atcctgaaac atcattcgcg tatcatcctg aatttcagcc agcggtgtgg
    13141 tgcccgctaa cacatcgcct gcggttggcg tttccagacc ggccagcagg tgcagcaggg
    13201 tacttttacc accaccgctg cggcccacca ccgccacaaa ctgacctgcc ggaatatgta
    13261 aatccagttg gttcaggacg atattttccg cgtaatgttt gcttactgca ttgagcaaca
    13321 atggtgtgcc ctggttcaga cgagcagtat tcatatcgtg gcctccttca aatgataagc
    13381 cgggttccag cgcaaccaga ggcgctctaa cagctgcgcg ctgacgtcag caagtttgcc
    13441 gagcagggcg taaagaataa tagcgactac caccacgtct gtttgcaaaa actctcgcgc
    13501 attcatcgcc agataaccaa tgccggaatt ggcagaaatg gtttcggcaa caatcagcgt
    13561 cagccacatc aggcccaacg caaaacgcac gccgaccata attgagggca gggcatcggg
    13621 caggatcaca tggataaaca gcggtatgcc ggataatcca tagctacgcg ccatctccac
    13681 cagcccgcga tcgatattac ggatgccatg ccaggtgttg atataaatgg ggaacagcgt
    13741 accgagcgcc accagaaaga ttttcgcgga ttcatcaatg ccaaaccaca aaatcaccag
    13801 cgggatcagc gccagatgcg gcacgttgcg caacatctga attgaggtat ccagcagcct
    13861 ttctccccag cgcgacagtc cgctaatcag tcccagaatc aggcccagcg atccgccaat
    13921 tgaaaagcca atcagcgccc gccaggaact gatcgccaga tgct
//
LOCUS       DAZPCS010000098        13248 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZPCS010000098 DAZPCS010000000
VERSION     DAZPCS010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13248
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.098"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(16..998)
                     /gene="kdgT"
                     /locus_tag="ACHUQ7_002743"
                     /pseudo
     CDS             complement(16..998)
                     /gene="kdgT"
                     /locus_tag="ACHUQ7_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
     gene            complement(1258..1878)
                     /gene="sodA"
                     /locus_tag="ACHUQ7_002744"
     CDS             complement(1258..1878)
                     /gene="sodA"
                     /locus_tag="ACHUQ7_002744"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFR1136081.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            2163..3197
                     /gene="rhaT"
                     /locus_tag="ACHUQ7_002745"
                     /pseudo
     CDS             2163..3197
                     /gene="rhaT"
                     /locus_tag="ACHUQ7_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(3194..4042)
                     /gene="rhaR"
                     /locus_tag="ACHUQ7_002746"
     CDS             complement(3194..4042)
                     /gene="rhaR"
                     /locus_tag="ACHUQ7_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFR1136082.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(4116..4952)
                     /gene="rhaS"
                     /locus_tag="ACHUQ7_002747"
     CDS             complement(4116..4952)
                     /gene="rhaS"
                     /locus_tag="ACHUQ7_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFR1136083.1"
                     /translation="MTVLHSVDFFPSCNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            5240..6709
                     /gene="rhaB"
                     /locus_tag="ACHUQ7_002748"
     CDS             5240..6709
                     /gene="rhaB"
                     /locus_tag="ACHUQ7_002748"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFR1136084.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIELMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            6706..7965
                     /gene="rhaA"
                     /locus_tag="ACHUQ7_002749"
     CDS             6706..7965
                     /gene="rhaA"
                     /locus_tag="ACHUQ7_002749"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFR1136085.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPRQAVWEMYC
                     QHHDTPAGSEWLESVRAYEKAILSQRG"
     gene            8270..9031
                     /gene="rhaD"
                     /locus_tag="ACHUQ7_002750"
     CDS             8270..9031
                     /gene="rhaD"
                     /locus_tag="ACHUQ7_002750"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFR1136086.1"
                     /translation="MKGWDERNGGNLTLRLDDADIALYHDNFHPQPRYIPLSQPMPLL
                     ANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGYHILWGLTNEAVPTSELPAHFL
                     SHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAVFTRQLWEGSTECLVVFPDGVG
                     ILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSGPTLDEAFGLIDTAEKSAQVLV
                     KVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            9041..9355
                     /gene="rhaM"
                     /locus_tag="ACHUQ7_002751"
     CDS             9041..9355
                     /gene="rhaM"
                     /locus_tag="ACHUQ7_002751"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFR1136087.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            9656..10102
                     /locus_tag="ACHUQ7_002752"
     CDS             9656..10102
                     /locus_tag="ACHUQ7_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HFR1136088.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQEFIHQLQQGDTDQVLALLNQTLSS"
     gene            10113..11564
                     /locus_tag="ACHUQ7_002753"
     CDS             10113..11564
                     /locus_tag="ACHUQ7_002753"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HFR1136089.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISLALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            11554..12624
                     /locus_tag="ACHUQ7_002754"
     CDS             11554..12624
                     /locus_tag="ACHUQ7_002754"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFR1136090.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGFVAPHALTEKQRQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKNCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKVNAFSQFRQVD"
     gene            12624..>13248
                     /locus_tag="ACHUQ7_002755"
     CDS             12624..>13248
                     /locus_tag="ACHUQ7_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFR1136091.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPQLKQRYERTCCLASRPGLGHFIDETEEKLVILLANLLRKDPFLIPLA
                     GITRDNLQHLSTACDNQHRWPLIQGDYLSSLILAIYALRNQLTDEWPQ"
BASE COUNT         3159 a         3458 c         3490 g         3141 t
ORIGIN      
        1 attaaggaag caggtttatt tcaccgtacc taaaatttcg attttcgctg ctctggcttt
       61 gactttacgt gaccagatag aggtaagaat tggcaccaga atcgaagtca caatgaccgc
      121 cgtcgctacc agcgaagttg ctgccggagc catcggttta aacgcaggca ccatttctgc
      181 aatcagcaca ggtgtcgcta ccgcggcccc tgcggaactg gaagcggcaa ttccggcagt
      241 gccatcgcca ccgccaatca atttatcggc gataatcaac ggaataccgg tcacgataat
      301 tactgccaca cccaacagga tccccagcaa accagtctgg gcaatcacag tcaaatcaat
      361 ggtattgccc agcgcgaagg caaagaatgg aatcagcgtt tgcaccgctt tgctgaaaaa
      421 ttctcgtaat tcagggtcaa gattccccag ggcaaagccc accaggaacg gtaatactgc
      481 gccgacgaaa acatgcggtt caaacgaggc aatcccggca gtgcccagaa taatcatcgt
      541 catgagcggc ccggactcca acgacatcaa cacaaatgcc cagcttcttc tttcgtaccg
      601 tactgctgca tgatggaagc gtaaagtccg ccgttggtca tatccatcgc cgccaccagc
      661 gccagcgttg aaagtccggc aaagaatcca acttcaacac catgttccgg aataatgcgc
      721 gaggcaattg ccgcaaccac ccacgcgacg gcaattttag ttaccaccag cgtaccggat
      781 ttacgcagta ccgttcccgt cgcgcttaat tttattgacg cccccatgca aaaaaaccac
      841 accgccagaa tgggcaccgt accggtaatc atcccgttgg tgaatgatcc aaaatatttc
      901 cccgcccccg gcgagaaggt gtggcacagt gcgccaagga atagcgggac gagcatcatc
      961 ccccccggga ttttctcaat cgagcgtttt atctgcattt ccatcacctg tcggcatatt
     1021 tttaagggct gatgcgtaat tcctcaatgg agcatcagtg ttgatggaaa cgaagataga
     1081 cattcattta atcaataaaa gtgatcaaaa tcacatcaat tgaaacgctg ttttattttt
     1141 ataggaccat tgtttttatt ttggaattga tcacaaaaaa accaccgccg ttggcgatgg
     1201 ttcatttttt aagctgatat gcggcctata cgcctcattg cagcaggcgg caaataatta
     1261 ttttttcgcc gcaaaacgtg ccgctgcttc gtcccagttc accacgttcc agaactcttt
     1321 gatgtagtcc ggacggcggt tctggaattt caggtagtaa gcatgttccc acacatccag
     1381 gcccagaatc gggaagccgg aagcgccaga aatagcttca cccatcagcg gagaatcctg
     1441 gttagcagta gaaaccaccg ccagtttatc gcctttcagc accagccatg cccagccgga
     1501 accaaagcgg gaagctgccg ctttttcaaa ttctgctttg aagttatcaa cggagccgaa
     1561 gtcacgttcg atagccgctt tcaggtcacc ctgcagggtg gtgccttttt tcagaccttt
     1621 ccagaacagg ctgtggttag cgtgaccgcc agcgttgttg cgcagtacgg ttttcttgtc
     1681 tgctggcagc tggtccagtt tggtgatcag ctcttcaacc ggcaggttgg caaattctgg
     1741 caggctttcc agcgccgcgt tggcgttgtt tacgtaggtc tgatggtgtt tggtgtggtg
     1801 gatttccatg gtctgcttat cgaagtgcgg ttccagggca tcgtaagcat acggcaggga
     1861 tggcagggta tagctcatat tcatctccag tattgtcggg cggccgattg ttaatgccgc
     1921 gtaagcagtt ggttcattat agttaattaa atgatattga aaatgattat caatgccgta
     1981 cttttcgtaa gggtatggtt ttgcaggaaa atgcccgaga tgtgaagcaa atcacccact
     2041 taatgccgtg attgccagta aatcgacaac ggcggcaaca ggcgaaaggt taatcgacag
     2101 cacgattttt acactcatct cgtcggagat gtgaagcgac gaaaaatgat gaggataaga
     2161 agatgagtaa cgcgattacg atggggatat tttggcattt gatcggcgcg gccagtgcag
     2221 cctgttttta cgctccgttc aaaaaagtaa aaaaatggtc atgggaaacc atgtggtcag
     2281 tcggtgggat tgtttcgtgg attattctgc cgtgggccat cagcgccctg ttactaccgg
     2341 atttctgggc gtattacagc tcgtttagtc tctctacgct gctgcctgtt tttctgttcg
     2401 gcgctatgtg ggggatcggt aatatcaact atggcctgac catgcgttat ctcggcatgt
     2461 cgatgggaat tggcatcgcc attggcatta cgttgattgt cggtacgctg atgacgccaa
     2521 ttatcaacgg caatttcgat gtgctgatta acaccgaagg cggacgcatg acgttactcg
     2581 gcgttctggt ggcgctgatt ggcgtaggga ttgtaactcg agccgggcag ttgaaagagc
     2641 gcaagatggg cattaaagcc gaagagttca atctgaaaaa agggctggtg ctggcggtga
     2701 tgtgcggcat tttctctgcc gggatgtcct ttgcgatgaa cgccgcaaaa ccgatgcatg
     2761 aagccgctgc cgcacttggc gtcgatccac tgtatgttgc tctgccaagc tatgttgtca
     2821 tcatgggcgg cggcgcgatc attaacctcg gtttctgttt tattcgtctg gcaaaagtga
     2881 aggatttgtc gctaaaagcc gacttctcgc tggcaaaacc gctgatcatt cacaatgtgt
     2941 tactctcggc actgggcggg ttgatgtggt atctgcaatt ctttttctat gcctagggcc
     3001 acgcccgcat tccggcgcag tatgactaca tcagttggat gctgcatatg agtttctatg
     3061 tattgtgcgg cggtatcgtc gggctggtgc tgaaagagtg gaacaataca ggacgccgtc
     3121 cggtaacggt gttgagcctc ggttgtgtgg tgattattgt cgccgctaac atcgtcggca
     3181 tcggcatggc gaattaatct ttctgcgaat tgagatgacg ccactggctg ggcgtcatcc
     3241 cggtttcccg ggtaaacacc accgaaaaat agttactatc ttcaaagcca cattcggtcg
     3301 aaatatcact gattaacagg cggctatgct ggagaagata ttgcgcatga cacactctga
     3361 cctgccgcag atattgattg atggtcattc cagtctgctg gcgaaattgc tgacgcaaaa
     3421 cgcgctcact gcacgatgcc tcatcacaaa atttatccag cgcaaaggga cttttcaggc
     3481 tagccgccag ccgggtaatc agcttatcca gcaacgtttc gctggatgtt ggcggcaacg
     3541 aatcactggt gtaacgatgg cgattcagca acatcaccaa ctgcccgaac agcaactcag
     3601 ccatttcgtt agcaaacggc acatgctgac tactttcatg ctcaagctga ccgataacct
     3661 gccgcgcctg cgccatcccc acgctaccta agcgccagtg tggttgccct gcgctggcgc
     3721 taaatcccgg aatcgctccc tgccagtcaa gattcagctt cagccgctcc gggcaataaa
     3781 taatattctg caaaaccaga tcgttaacgg aagcgtagga gtgtttatcg tcagcatgaa
     3841 tataaaagag atcgccacgg gtaatgcgat aaggacgatc gttgagtaca tgcagaccat
     3901 taccgcgcca gacaatcacc agctcacaaa aatcatgtgt atgttcggca aagacatctt
     3961 gcggataacg gtcagccaca gcgactgcct gctggtcgct ggcaaaaaaa tcatctttga
     4021 gaagttttaa ctgatgcgcc accgtggcta cctcggccag agaacgaagt tgattattcg
     4081 caatatggcg tacaaatacg ttgagaagat tcgcgttatt gcagaaagcc atcccgtccc
     4141 tggcgaatat cacgcggtga ccagttaaac tctcggcgaa aaagcgtcga aaagtggtta
     4201 ctgtcgctga atccacagcg ataggcgatg tcagtgacgc tggcctcgct gtggcgtagt
     4261 agatgtcggg ctttcatcag tcgcaggcgg ttcaggtatc gctgaggcgt cagtcccgtt
     4321 tgctgcttaa gctgccgatg tagcgtacgc agtgaaagag aaaattgatc cgccacggca
     4381 tcccaattca cctcatcggc aaaatggtcc tccagccagg ccagaagcaa gttgagacgt
     4441 gatgcgctgt tttccaggtt ctcctgcaaa ctgcttttac gcagcaagag cagtaattgc
     4501 ataaacaaga tctcgcgact ggcggtcgag ggtaaatcat tttccccttc ctgctgttcc
     4561 atctgtgcaa ccagctgtcg cacctgctgc aatacgctgt ggttaacgcg ccagtgagac
     4621 ggatactgcc catccagctc ttgtggcagc aactgattca gcccggcgag aaactgaaat
     4681 cgatccggcg agcgatacag cacattggtc agacacagat tatcggtatg ttcatacaga
     4741 tgccgatcat gatcgcgtac gaaacagacc gtgccaccgg tgatggtata gggctgccca
     4801 ttaaacacat gaatacccgt gccatgttcg acaatcacaa tttcatgaaa atcatgatga
     4861 tgttcaggaa aatccgcctg cgggagccgg ggttctatcg ccacggacgc gttacaagac
     4921 ggaaaaaaat ccacactatg taatacggtc atactggcct cctgatgtcg tcaacacggc
     4981 gaaatagtaa tcacgaggtc aggttcttac cttaaatttt cgacggaaaa ccacgtaaaa
     5041 aacgtcgatt tttcaagata cagcgtgaat tttcaggaaa tgcggtgagc atcacatcac
     5101 cacaattcag caaattgtga acatcatcac gttcatcttt ccctggttgc caatggccca
     5161 ttttcctgtc agtaacgaga aggtcgcgaa ttcaggcgct ttttagactg gtcgtaatga
     5221 aattcagcag gatcacatta tgacctttcg caattgtgtc gccgtcgatc tcggcgcatc
     5281 cagtgggcgc gtgatgctgg cgcgttacga gcgtgaatgc cgcagcctga cgctgcgcga
     5341 aatccatcgt tttaacaatg ggctgcatag ccaaaacggt tatgtcacct gggatgtgga
     5401 tagcctggaa agtgccattc gccttggatt aaacaaggtg tgcgaggaag ggattcgtat
     5461 cgatagcatt gggattgata cctggggcgt ggactttgtg ctgctcgacc aacagggtca
     5521 gcgtgtgggc ctgcccgttg cttatcgcga tagccgcacc aatggcctga tgccgcaggc
     5581 acaacagcaa ctcggcaaac gcgatattta tcagcgtagc ggcatccagt ttctaccctt
     5641 caatacgctt tatcagttgc gtgcgctgac ggagcaacaa cctgaactta ttccacacat
     5701 tgctcacgct ctgctgatgc cggattactt cagttatcgc ctgaccggca agatgaactg
     5761 ggaatatacc aatgccacca ccacacaact ggtcaatatc aatagcgacg actgggacga
     5821 gtcgctactg gcgtggagcg gggccaacaa agcctggttt ggtcgcccga cgcatccggg
     5881 taatgtcatt gggcactgga tttgcccgca gggtaatgag attccggtgg tcgccgttgc
     5941 cagccatgat accgccagcg cggttatcgc ctcgccgtta aacggttcac gcgccgctta
     6001 tctctcttct ggcacctggt cattgatggg cttcgaaagc cagacgccat ttaccaatga
     6061 cacggcgctg gcagccaaca tcaccaatga aggcggggcg gaaggtcgct atcgggtgct
     6121 gaaaaatatt atgggcttat ggctgcttca gcgagtgctt caggaacggc aaatcaacga
     6181 tctcccggcg cttatcgccg cgacacaggc acttccggcc tgccgcttca tcatcaatcc
     6241 caatgacgat cgctttatta atcctgacga gatgtgcagc gaaattcagg ctgcgtgtcg
     6301 ggaaacggcg caaccgatcc cgggaagtga tgctgaactg gcgcgctgta ttttcgacag
     6361 tctggcgctg ctgtatgccg atgtgttgca tgagctggcg cagctgcgcg gtgaagattt
     6421 ctcgcaactg catattgtcg gcggcggctg ccagaacacg ctgctcaacc agctatgcgc
     6481 cgatgcctgc ggtattcggg tgatcgccgg gcctgttgaa gcctcaacgc tcggcaatat
     6541 cggcatcgag ttaatgacgc tggatgaact caacaatgtg gatgatttcc gtcaggtcgt
     6601 cagcaccacc gcgaatctga ccacctttac ccctaatcct gacagtgaaa ttgcccacta
     6661 tgtggcgcag attcactcta cacgacagac aaaggagctt tgcgcatgac cactcaactg
     6721 gaacaggcct gggagctggc gaaacagcgt ttcgcggcgg tggggattga tgtcgaggag
     6781 gcgctgcgcc aacttgatcg tttacccgtt tcaatgcact gctggcaggg cgatgatgtt
     6841 tccggttttg aaaacccgga aggttcgctg accgggggga ttcaggccac aggcaattat
     6901 ccgggcaaag cgcgtaatgc cagtgagcta cgtgccgatc tggaacaggc tatgcggctg
     6961 attccggggc cgaaacggct taatttacat gccatctatc tggaatcaga tacgccagtc
     7021 tcgcgcgacc agatcaaacc agagcacttc aaaaactggg ttgaatgggc gaaagccaat
     7081 cagctcggtc tggattttaa cccctcctgc ttttcgcatc cgctaagcgc cgatggcttt
     7141 acgctctcgc atcccgacga cagcattcgc cagttctgga ttgatcactg caaagccagc
     7201 cgtcgcgttt cggcctattt tggcgagcaa ctcggcacac catcggtgat gaacatctgg
     7261 atcccggatg gtatgaaaga tatcaccgtt gaccgtctcg ccccgcgtca gcgtctgctg
     7321 gcagcactgg atgaggtgat cagcgagaag ctaaaccctg cgcaccatat cgacgccgtt
     7381 gagagcaaat tgtttggcat tggcgcagag agctacacgg ttggctccaa tgagttttac
     7441 atggggtatg ccaccagccg ccagactgcg ctgtgcctgg acgccgggca cttccacccg
     7501 actgaagtga tttccgacaa gatttccgcc gccatgctgt atgtaccgca gttgctgctg
     7561 cacgtcagcc gtccggttcg ctgggacagc gatcacgtag tgctgctgga tgatgaaacc
     7621 caggcaattg ccagtgagat tgtgcgtcac gatctgtttg accgggtgca tatcggcctt
     7681 gacttcttcg atgcctctat caaccgcatt gccgcgtggg tcattggtac acgcaatatg
     7741 aaaaaagccc tgctgcgtgc gttgctggaa cctaccgctg agctgcgcaa gctggaagcg
     7801 gcgggcgatt acactgcgcg tctggcactg ctggaagagc agaaatcgtt gccgcggcag
     7861 gcggtctggg aaatgtattg ccaacatcac gatacgccag caggtagcga atggctggag
     7921 agcgtgcggg cttatgagaa agcgattttg agccagcgtg ggtaaacact gccggatgcg
     7981 gcacgagcgc cttatccggc ctacgggtcg aatgtccatg cgggctgtca ccctcacccc
     8041 ggccctctcc ctgaaagggc gagggagaaa aacatagcct cgtgcggcaa cggagctgaa
     8101 caccgctccg gacagtcccc tcgcccctac ggggagaggg ttagggtgag gggaacaagc
     8161 cgcaccatgt ctaaacaagg caacctaatt tacaggaaca cagaacatgc aaaacattac
     8221 ccagtcctgg tttgtccagg gaatgatcaa agccaccacc gatgcctagc tgaaaggctg
     8281 ggacgagcgc aacggcggca acctgacgct gcgcctggat gacgccgata tcgcgcttta
     8341 tcacgacaac ttccacccac aaccgcgcta tattccgctc agccaaccca tgcctttact
     8401 ggcaaatacg ccgtttattg tcaccggctc cggcaaattc ttccgtaacg tccagctcga
     8461 tcctgcagct aacttaggcg tcgtaaaagt cgacagcgat ggcgcgggct accatattct
     8521 ctgggggtta accaacgaag ccgttccaac ttctgaactt ccggctcact ttctctccca
     8581 ctgcgagcgc attaaagcca ccaacggtaa agatcgggtg atcatgcact gccacgccac
     8641 caacctgatc gccctcacct atgtactgga aaacgacacc gcggtcttta ctcgccaact
     8701 gtgggaaggc agcaccgaat gtctggtagt attcccggat ggcgttggca ttctgccgtg
     8761 gatggtgccc ggcacggacg aaatcggcca ggcgaccgca caagagatgc aaaaacattc
     8821 gctggtgttg tggcccttcc acggtgtgtt cggttccgga ccgacgctgg atgaagcctt
     8881 tggtttaatc gacaccgcag aaaaatcagc acaagtatta gtgaaggttt attcgatggg
     8941 cggcatgaaa cagaccatca gccgtgaaga gttgatagcg ctcggcaagc gcttcggcgt
     9001 tacaccacta gccagtgcgc tggcgctgta aggagcaaac atgatccgca aagcctttgt
     9061 catgcaggta aaccccgacg cccacgaaga gtatcagcgt cggcataatc ccatctggcc
     9121 ggaactggaa gcagtgttga aatctcacgg tgtgcataac tacgccattt acctcgacaa
     9181 agcgcgtaat ctgctgtttg ccatggtaga gattgaatct gaagaacggt ggaatgcggt
     9241 agccagtact gatgtttgcc aacgttggtg gaaatatatg accgatgtta tgcccgctaa
     9301 cccagataac agcccggtga gtagcgagct gcaagaagtg ttttacctgc cgtaattgcc
     9361 caatgtgccg gagatgcctc cggcacaata ttttaaattt cgatttgaaa aattaaacat
     9421 attacatctc ccggtaagtt atatttccct gatacattgt gagtaaatca caaaaataat
     9481 gaataaccca ttaatgattc atgtggttta tttaaataac cattatgtgc attactccgc
     9541 aaatctgacc tttcactctg tctcaaattg tcattgttta ccgacatatc gcctctcatc
     9601 acttaatcgc tccaccatta caattgatta aaatatattt tatggagtcc gattaatggc
     9661 agctcttact gcaagctgta ttgacctgaa tattcagggc aatggcgctt attccgttct
     9721 gaagcagttg gcgacaatag cgttacaaaa cggttttatc accgactcac accagttcct
     9781 gcaaaccctg ctgctgcgcg aaaaaatgca ctctactgga tttggttccg gtgtcgcagt
     9841 gccgcacggt aaaagcgcct gcgttaaaca accgttcgta ttattcgccc gcaaagcgca
     9901 ggctattgac tggaaagcca gcgatggcga agacgtcaat tgctggatct gcctcggcgt
     9961 gccgcaaagc ggcgaagagg atcaggtcaa aatcatcggc acactgtgtc gcaaaattat
    10021 tcaccaggaa tttattcatc aactgcaaca gggcgatacc gaccaggtgc tggccttgtt
    10081 aaatcaaacc ctcagctcat aaggaagtgg cgatggagtc atccttacgt attgtcgcga
    10141 tcaccaactg ccccgccggg atcgctcaca cctacatggt ggcggaagcc ctggaacaga
    10201 aagcccgttc tctcggccat accataaaag tggaaactca agggtccagt ggcgttgaaa
    10261 accgcttatc cagcgaagag attgccgctg ccgattacgt cattcttgct accgggcgtg
    10321 gcctgagcgg tgatgatcgc gcgcggtttg ccgggaagaa agtttatgag attgccatct
    10381 ccctggcgtt gaaaaatatc gaccagattt tcagcgaatt accgacaaac tcgcagcttt
    10441 ttgccgcaga tagcggcgtg aagcttggca aacaggaagt gcaaagcggc agcgtgatga
    10501 gccacctgat ggcgggcgtc tctgcggcgc tgccgtttgt catcggcggt ggtatcctgg
    10561 tggcgcttgc caacatgctg gtgcagttcg gtttgccgta taccgatatg tcgaaaggcg
    10621 cgccgtcgtt tacctgggta gttgaatcca tcggttacct tggcttcacc tttatgatcc
    10681 ccatcatggg cgcttatatt gcctcgtcga ttgccgataa accggctttc gctccggcgt
    10741 ttctggtttg ctatctggcg aacgacaaag cgctgctcgg cacccagtcg ggtgcgggat
    10801 tcctcggcgc ggtggtgctg gggctggcga ttggctattt cgtcttctgg ttccgcaaag
    10861 tgcgccttgg caaagcatta caaccgctgc tcggttcaat gctgatcccg ttcgttactc
    10921 tgctggtttt cggcgtgctc acttactacg ttatcgggcc ggtgatgtcc gacctcatgg
    10981 gcgggctgct ccacttcctg aataccattc ctccatcaat gaagtttgcg gcagcgtttc
    11041 tggtcggtgc gatgctggcg ttcgatatgg gcggccccat caataaaacc gcctggttct
    11101 tctgcttctc actgctggaa aaacacattt atgactggta cgccatcgtc ggcgttgtcg
    11161 cgttgatgcc gcccgttgcg gcgggtcttg cgacgttcat tgcgcccaaa ctgtttaccc
    11221 gacaggaaaa agaggctgcc agtagtgcca ttgtggtcgg tgccacagtc gcaaccgaac
    11281 cggctatacc ttacgctctg gcagctccgt tgccgatgat taccgccaat acgctggcgg
    11341 gcggcattac cggcgtgctg gtgatcgctt tcggaatcaa acgtctggca cctggtttgg
    11401 gtatctttga cccgctgatc ggcctgatgt cgccggtagg ttcgttttat ctggtgctgg
    11461 cgattggcct cgcgctgaac atttcattca ttatcgtcct gaaaggattg tggttacgcc
    11521 gcaaagcgaa agccgcgcaa caggagcttg tccatgaaca ttgagttact gcaacagttg
    11581 tgcgaagcca gcgccgttag cggcgatgaa caggaagttc gcgacatcct gataaacacg
    11641 ctggaacctt gcgttaatga aatcaccttt gatggtctgg gcagctttgt tgcccgtaag
    11701 ggaaataaag gtccaaaagt tgccgttgtc gggcatatgg atgaagtcgg ctttatggtc
    11761 acccacatcg acgagagcgg ttttctgcgt ttcaccacca ttggtggctg gtggaatcag
    11821 tcgatgctca accatcgggt aaccatacgc acacacaagg gagtgaaaat ccctggtgtg
    11881 attggtttcg tcgcgcctca tgcgttaacg gaaaagcaaa ggcaacagcc gctgtcattt
    11941 gatgagatgt tcattgatat tggcgcaaac agtcgcgatg aggtggaaaa acgcggtgtg
    12001 gcgatgggcg attttattag cccggaagcc aattttgcct gctggggcga agataaagtg
    12061 gtcggcaagg cgctggataa ccgcatcggc tgcgcaatga tggctgaact attgcagacg
    12121 gtgaataatc ccgaaattac gctgtatggc gtcggcagcg tggaagaaga agttgggcta
    12181 cgcggggcac aaacctcggc ggaacacatt aaaccggatg tagtgatcgt gctggatacc
    12241 gctgtcgcag gcgatgttcc gggcattgat aacattaaat acccgctgaa actgggccag
    12301 gggccggggc tgatgctgtt tgacaagcgc tacttcccca accagaaact ggtagcagcg
    12361 ttaaaaaact gtgccgcaca taacgattta ccgctgcaat tttccaccat gaaaaccgga
    12421 gcgacggatg gcgggcgcta caacgtgatg ggcggcgggc gtccggttgt tgcgctgtgc
    12481 ctgccaaccc gctatctgca cgctaacagc ggcatgattt caaaagccga ttacgatgct
    12541 ctgctcacgt tgatacgggg ttttctgacg accttaaccg cggagaaagt caacgcgttt
    12601 agccagttcc gtcaggtgga ttaatgctaa acgaacgcca gttaaagatt gtcgatctgc
    12661 tggagcaaca gccgcgcacg cctggtgagc tggcgcaaca gactggcgtt tcaggcagga
    12721 ccatcctgcg tgatattgac tatctcaact ttacccttaa cggcaaagcc cgcattttcg
    12781 ccagtggcag tgcgggctat cagctggaaa tcttcgagcg ccgcagcttt tttcagttgc
    12841 tgcaaaagca cgataacgac gatcggctgc tggcgctgtt attactgaat actttcactc
    12901 cccgtgcgca actcgcctcg gcgcttaatt tgccagaaac gtgggtagca gagcgtctgc
    12961 cccagttaaa acagcgttat gaacgcactt gttgcctggc cagccgccct ggtttgggcc
    13021 atttcattga tgagacagaa gagaaactcg ttatcttgct ggcgaacttg ctgcgcaaag
    13081 atccgttttt aattccgctg gcgggcataa cacgagacaa ccttcagcat ttatccacgg
    13141 cctgcgacaa ccaacaccgc tggccgctca tacagggtga ttatctctcc agcctgattc
    13201 tggcgattta cgccctgcgt aatcaactga ccgatgagtg gccgcaat
//
LOCUS       DAZPCS010000099        13125 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZPCS010000099 DAZPCS010000000
VERSION     DAZPCS010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13125
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.099"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(109..429)
                     /locus_tag="ACHUQ7_002756"
                     /pseudo
     CDS             complement(109..429)
                     /locus_tag="ACHUQ7_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
     gene            complement(635..1354)
                     /locus_tag="ACHUQ7_002757"
                     /pseudo
     CDS             complement(635..1354)
                     /locus_tag="ACHUQ7_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
     gene            complement(1370..1702)
                     /gene="cusF"
                     /locus_tag="ACHUQ7_002758"
     CDS             complement(1370..1702)
                     /gene="cusF"
                     /locus_tag="ACHUQ7_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFR1136092.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(1726..3108)
                     /gene="cusC"
                     /locus_tag="ACHUQ7_002759"
     CDS             complement(1726..3108)
                     /gene="cusC"
                     /locus_tag="ACHUQ7_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFR1136093.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPVMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLDDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEIYL
                     YTALGGGWQQ"
     gene            3265..3948
                     /gene="cusR"
                     /locus_tag="ACHUQ7_002760"
     CDS             3265..3948
                     /gene="cusR"
                     /locus_tag="ACHUQ7_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFR1136094.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKLLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            3938..5380
                     /gene="cusS"
                     /locus_tag="ACHUQ7_002761"
     CDS             3938..5380
                     /gene="cusS"
                     /locus_tag="ACHUQ7_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFR1136095.1"
                     /translation="MVSKPFQRPFSLATRLTFFISQATIAAFFAFAWIIIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPA"
     gene            5530..7767
                     /gene="nrfB"
                     /locus_tag="ACHUQ7_002762"
     CDS             5530..7767
                     /gene="nrfB"
                     /locus_tag="ACHUQ7_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HFR1136096.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGNGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRVLKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            7754..10722
                     /gene="nfrA"
                     /locus_tag="ACHUQ7_002763"
                     /pseudo
     CDS             7754..10722
                     /gene="nfrA"
                     /locus_tag="ACHUQ7_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
     gene            10723..11613
                     /locus_tag="ACHUQ7_002764"
     CDS             10723..11613
                     /locus_tag="ACHUQ7_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFR1136097.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            11796..12557
                     /gene="envY"
                     /locus_tag="ACHUQ7_002765"
     CDS             11796..12557
                     /gene="envY"
                     /locus_tag="ACHUQ7_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HFR1136098.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAT
                     NHSKQQSSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQRQKVMW"
     gene            complement(12603..12679)
                     /locus_tag="ACHUQ7_002766"
     tRNA            complement(12603..12679)
                     /locus_tag="ACHUQ7_002766"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12643..12645),aa:Arg,seq:tct)
     gene            12922..>13125
                     /locus_tag="ACHUQ7_002767"
     CDS             12922..>13125
                     /locus_tag="ACHUQ7_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFR1136099.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFL"
BASE COUNT         3219 a         3405 c         3330 g         3171 t
ORIGIN      
        1 atccctccgg cagaagcaga aaaagcttat tatgcttcca tcggaaacga tgatctggca
       61 gcctgagttc acagataaaa cactctccag gaaacccggg gcggttcagt tgtcgaacca
      121 gtagtcgaga cactgggcga ccgtcatgga gttctccacc ttctcttcaa aataagtgcg
      181 tggatccgtt cctgagaaat agagctttcg caagtcagca gtgatctgtc tggcatcctt
      241 caaagacagg gatgggtatc gaccaagccc aagtcgatta ggcttgccat gccagcgata
      301 gcggtactgg aactggatga cccccttcgg tgaaattcgt acgctgaggc catcagcatc
      361 agccaattct tgtgggccag aatatggttt accataaata gtacgcagtt ttgtgtcgct
      421 tatagccata aataatattt ggtacgctca gaaataatgg tttggtacac atctggtaca
      481 caatatcaca tggacaacag cgcacagtaa acaactatat ttgacgttcg tagacatact
      541 caggtgaaaa aaaggttgtt tttcgagtta taacagacag ttaattaact acagtggaca
      601 atgctagaca atgacagaca cagataagca acctaccttc ctctttcgcg accacgttat
      661 ctttggcgat attcattccc gcgcgcagat caaacgcggt gatcacgcca tcaatgggcg
      721 ctttgagcgt aaagcgagtc tggatttttt gcgtggcgat caggtggcga atatccgcct
      781 ccggcattcc cgccagtcgc agccgctcaa gaatgccttc agtctgggtc gccgtaccgc
      841 cggtttcgcg caacagtaaa tactcactct gcgcttccac ccagtcggga atggtcaggt
      901 cgagaagcgg tgtgcccttt tgtactttat cgcccacggt aagcggatac accttgtcga
      961 taaacccggc agcgcgagcc tgcacaatgg catactgata ctcgttgtaa ctgacattcg
     1021 tcgggaaact ctgggcaaaa gtcagcggcc cgcgcgtgac ggtagccgtt ttcaccccca
     1081 gattctgcgt ctgagtcggg tcaatgcgca caccagacgc agaactctct tcatcggcat
     1141 atttcggcac cagatccata tccataaacg gcgatttacc tggtttatcg aaccgcgtat
     1201 tgggatacat cgggtcgtac cagaataaga ttttacgttc tgcggtcggc attttttctg
     1261 cgtgcggttc cgcctggtca aaccaggtaa aacctgccgc tgaaataata ccgcccgcga
     1321 tcatgctgcc gataataagc gcgatttttt tcatctcatt aaacctgggt tactggctga
     1381 ctttaatatc ctgtaataaa gaaaggttgc cctgctggac aaaattaaac gccactttgt
     1441 cgccggtttt aatttcactc attttcgtct gcggggtgat ggtaaagcgc atggtcatct
     1501 ccggccagtt gacggcagca atcggatcgt gatggatggt gatttttttg ctttccagat
     1561 caacaccttt taccacaccg gtggcactaa tgacctgtgg ttgtgcttcg ctcatggttt
     1621 catgatgatg ttcgttagcc tgggcattaa agccaataac ggtaaacaga ctgaacattg
     1681 cgacttgcag tgcttttttc atttattctc tcctggagtt aaaaattatt gctgccaacc
     1741 gccccctaag gcggtataca aataaatttc gttaacctga cgggcataat tcaggtcaag
     1801 taaagtttgt cgggttgcaa ataaagaacg ctcggcatcc agcacttcca gataacttac
     1861 cgcgccgtgc tgatataatg cccgcgcccg ttgcagggta atttgcagcg acgccagata
     1921 acgctgctgg gcgctgattt gatcgtccag gctttgacgt aatgccagcg catctgccac
     1981 ctctttaaag gcgttctgga ttttctgttc ataattcacc accgactgct gctggcgaat
     2041 ttcggcgata tccagattgg cctggttgcg tccggcatta aaaatgggga tctcaatttt
     2101 aggaataaaa ttccacatcc cgctgctagc gttaaataat gacgacagat cgctgctggc
     2161 ggttgatatt ccgctggtca ggctgataga cgggaaaaat gccgcacgcg cagcaccaat
     2221 attagcatta gccgccatta acgcgtgttc agcttccata atatccgggc gctgcaataa
     2281 gatttgcgac gacaagcccg ccggtaattt aacgctttgc aggctgccgc tgtttactgt
     2341 ctgcgcttgc ggtagcttgc cgtagcttcc caataacagt tgcaatgcat tattcgcctg
     2401 cgccagttcc ccctgacgtt tagcgatgtc actgcgggta ctttctatca ccccgcgagc
     2461 ctgttccagc gccagaacat tgctgctacc ggtcaacagt tgtttttcga caaacgcata
     2521 tgactgctga taattacgca gcgtttcttc ggctatttgc aattgcgcat acgccagctg
     2581 ctgattgaaa tagctttgcg cgacattaga aaccagcaaa atatgcaccg cgcgctgggc
     2641 ttcctcagtg gctaaataat tttggcgctc ggcttcgctc atgttcttta agcgaccgaa
     2701 aaaatcgaga tcaaagctgg cgttaaggcc agtcgagaac tcccgcgtcg tggctgaatc
     2761 gcctttaaga ttgccgctcc agctgccgct gccctcgcca ttgagctgtg ggtagcggtc
     2821 ggcatcggtc agacgatatt gcgcccgtgc ctcctgcact ttcagcgtcg ccatgcgcaa
     2881 atcccggtta ttcaccagcg cctcgctaat cagcgtcttc acctgattat caacaaaaaa
     2941 ggtgcgccag cccacgttct ggtagttatc cgctgcgtta accaggccgt tctggctgag
     3001 tgagaactgc tgcggtacgg gcattaccgg acgctgataa tccggtgcca gtgaacaacc
     3061 agttagcgca agggccacac aaaatggcag aagtttacaa ggagacatag gctcataatt
     3121 tctggtgatt ttatgtcgcc aactttactc gccaggctct gattttccgg tgacaggaaa
     3181 atgacaaaat tgtcattttg ccaataagcg attgccatct gatcccgcta ctctagaatt
     3241 gcccgggcaa catgcggagg aaatatgaaa ctgttgattg tcgaagatga aaagaaaacc
     3301 ggagaatact tgaccaaagg gttaaccgaa gccggttttg tggtcgattt ggccgacaac
     3361 gggctgaatg gctaccatct ggcgatgacc ggtgattatg acctgataat cctcgatatt
     3421 atgctgccag acgtgaacgg ctgggatatc gtgcgcatgt tgcgctccgc caataaaggg
     3481 atgccgattc tgttgcttac cgcgcttggc accattgaac atcgcgtcaa ggggctggag
     3541 ttgggggcag atgactacct ggtgaaaccg ttcgcttttg ctgaactgct ggcgcgggtg
     3601 cgcactctcc tgcggcgcgg ggcggcggtg attatcgaaa gtcagtttca ggttgccgat
     3661 ctgatggtcg atctcgtcag ccgcaaagtc acccgcagcg gcacgcgcat cactctgacc
     3721 agtaaagaat ttactctgct ggagtttttt ctccgccatc agggcgaagt gctgccccgc
     3781 tcgcttatcg cctcgcaggt atgggacatg aatttcgaca gcgacactaa cgccattgat
     3841 gtggcggtga agctgctacg cggcaaaatc gacaacgact ttgagccgaa gctgattcaa
     3901 accgtgcgcg gcgtgggtta catgcttgag gtgccggatg gtcagtaagc catttcagcg
     3961 cccgttttcg ctggcaaccc gcctgacctt ttttatcagc caggccacca tcgcggcgtt
     4021 tttcgccttt gcatggatca taatccactc agtaaaagtg cattttgccg agcaggacat
     4081 taatgattta aaagagatta gcgccaccct tgaacgggta ctaaatcacc ctgacgaaac
     4141 gcaagcccga cgcttaatga cgctggaaga tatcgtcagt ggttattcca acgtgttgat
     4201 ttccctggca gatagccacg gcaaaacggt gtatcactcc cccggtgcgc ctgatatccg
     4261 cgagtttgca cgtgatgcca tacccgataa agacgcgcgg ggtggcgagg tgtatctcct
     4321 ttccggcccg acgattatga tgccaggcca cggtcacggg catatggaac acagcaactg
     4381 gcggatgatt aacttgccgg ttggcccgtt ggtggacggc aaaccgattt atacgctcta
     4441 catcgcgctg tcgatcgatt ttcatcttca ttacataaat gatttgatga ataaacttat
     4501 tatgaccgca tcggtaatca gcatcctgat cgtctttatc gtgctgttgg cggtacataa
     4561 aggtcacgcg ccgatccgca gcgtcagccg tcaaatccag aatatcacct cgaaagatct
     4621 cgacgttcgc cttgacccgc agaccgtgcc gattgagctg gaacagctgg tactgtcgtt
     4681 caaccatatg atcgagcgta ttgaggatgt ctttactcgc cagtccaatt tctcagcgga
     4741 tatcgcccac gaaattcgca caccaattac taatctcatt acgcaaacgg aaatcgccct
     4801 cagccagtcg cgcagccaga aggagctgga agatgtgctc tactctaatc tcgaagagct
     4861 gacgcgaatg gcgaaaatgg tcagcgatat gctgtttctc gctcaggccg ataacaacca
     4921 gctaatcccc gaaaagaaaa tgctcaacct ggcggatgaa gtcggcaaag tgttcgattt
     4981 tttcgaggcg ctagcggaag atcgcggcgt ggagttgcgg tttgttggcg acaaacgtca
     5041 ggttgcgggc gatccgttga tgctgcgtcg ggcgctaagc aacctgcttt ctaatgccct
     5101 tcgttatacg ccaaccagag agacaattgt agtacgctgt cagacggtcg atcaccaggt
     5161 gcaagtttcc gtcgaaaacc ccggtacgcc cattgcgccc gagcacttac cgcgattgtt
     5221 tgaccgtttc tatcgcgttg acccctcccg ccagcgaaaa ggtgaaggta gcggtattgg
     5281 gctggcgata gtgaaatcga ttgttgtcgc gcataaaggc acggttgcgg taacgtcaga
     5341 tgcgcggggg acaaggtttg ttatcacatt acccgcttaa ttattcagca aacggcaact
     5401 tttataacca gtgtaaaaat aacgtgccgc aatagtcata catattaatg gtaaaaagct
     5461 gtcacaattc ataaaaaacc ttaatatacg ccaccctaaa cataaccagc gttaatgtaa
     5521 ggtttttgtg tggactggct tcttgatgtt tttgctacct ggctctacgg cttaaaagta
     5581 atcgcgataa cgttagcggt catcatgttc atcagcgggc tggacgattt ttttattgat
     5641 gtcgtctact gggtacgccg cattaaacgc aagttgagtg tttatcgccg ctacccgcga
     5701 atgagttacc gcgaactgta taaaccagat gaaaaaccgt tagcgattat ggttccggcg
     5761 tggaatgaaa cgggcgtcat cggcaatatg gccgagctgg cggcgaccac gctcgactac
     5821 gaaaactatc atatctttgt tggcacctac cccaacgacc ccgatactca acgtgatgtt
     5881 gacgaagtgt gcgctcgctt tccgaacgtg cataaggtag tctgcgcgcg tcctggtccc
     5941 accagcaaag ccgactgcct gaacaacgtg ctggacgcca tcacccagtt tgaacgtagc
     6001 gccaatttcg cttttgctgg ttttattctg catgacgccg aagatgtgat ttcaccgatg
     6061 gaattgcgtc tgttcaacta tctggtcgag cgtaaagatc tgattcagat cccagtgtat
     6121 ccgtttgaac gcgaatggac gcacttcacc agcatgactt acattgatga gttttcagaa
     6181 ctgcatggca aagatgttcc ggtgcgtgaa gccctcgccg gacaagtgcc cagcgcaggc
     6241 gtcggcacct gtttcagccg ccgcgccgtg accgcactgt tagctgacgg taacggtatt
     6301 gctttcgacg tgcagagtct tactgaagat tacgacattg gcttccgcct gaaagaaaaa
     6361 ggtatgacgg aaatttttgt ccgttttccg gtggtggacg aagccaaaga acgcgagcag
     6421 cgtaaatttt tacagcacgc acggacgtca aacatgatct gcgtgcgcga atatttcccc
     6481 gatacctttt cgactgcggt tcgacaaaaa tctcgctgga tcatcggcat tgttttccag
     6541 ggctttaaaa cccataaatg gacctccagc ctgacgctga actactttct ctggcgcgac
     6601 cgcaaagggg caatcagtaa ctttgtcagc ttcctcgcga tgctggtgat gatccagctt
     6661 ttgctgttgc tggcgtatga aagtttgtgg cccgatgcct ggcatttcct ttctattttt
     6721 agcggcagcg catggttaat gaccctgctg tggctaaact ttggcttgat ggttaaccgc
     6781 atcgtgcagc gagtgatttt cgtcactggc tactacggcc tcacgcaggg cctgctttcc
     6841 gtcctgcgtc ttttctgggg caacctgatt aactttatgg ctaactggcg cgtgctgaaa
     6901 caggttcttc agcacggcga tccgcgtcgc gtcgcgtggg ataaaacaac gcatgacttc
     6961 cccagcgtca ctggcgatac ccgctcgttg cgcccgttag gtcaaattct gctggaaaat
     7021 caggtcatca ctgaagaaca actcgataca gcactgcgta atcgcgtcga aggtctacgc
     7081 ctgggcggtt caatgctgat gcaggggctg attagcgccg agcagctggc acaggcgctg
     7141 gcagagcaaa acggcgtggc gtgggaatcc atcgatgcct ggcagatccc gtcctcgctg
     7201 attgccgaaa tgccagcctc cgtagcgctg cattatgcag tactgccgct gcgtctggaa
     7261 aatgatgagt taattgtcgg cagtgaagat ggtattgacc cggtttcgct ggcggccctg
     7321 acgcgtaaag tcggacgcaa agtgcgttac gtcattgttc tgcggggaca aattgtcaca
     7381 gggttacgcc actggtatgc acgccgacgc ggtcacgatc cgcgggcaat gttgtacaat
     7441 gcggttcagc atcagtggct cacggaacag cagaccggtg aaatctggcg gcaatatgtg
     7501 ccgcatcagt tcctgttcgc cgaaatactg accacgctcg gtcatattaa tcgttcagca
     7561 attaacgtgt tgttattgcg ccatgaacgc agttctctgc cgctcggcaa gtttttggtc
     7621 accgaaggcg ttatcagcca ggaaacgttg gatcgcgtcc tgacaattca acgcgaatta
     7681 caagtttcga tgcaatcact attactcaaa gcaggtttaa acacagaaca ggttgcgcaa
     7741 ctggagtccg aaaatgaagg agaataacct taatcgcgtc atcggatggt ctggtttact
     7801 gctgacgtct ttattgagta ccagcgcact cgcagacaat atcggcacca gcgcagaaga
     7861 gctggggctg agcgattatc gccattttgt tatttatccc cgtctcgata aggcgctgaa
     7921 ggcacagaaa aataacgacg aagcaaccgc catccgcgaa tttgaatata tacaccaaca
     7981 ggtgccggat aatattccgc tgactttata ccttgcggaa gcctatcgtc attttggtca
     8041 tgatgaccgg gcgcggctgt tgcttgagga tcaactgaaa cgtcacccag gagatgcccg
     8101 acttgagcgc agtctggcgg ctattccagt tgaagtgaaa agcgttacga ctgttgaaga
     8161 actgcttgcc cagcaaaaag cgggcgacgc tgcgccgacc ctgcgttgtc gcagtgaagt
     8221 cgggcagaat gccctgcggc tggcacaatt acctgtcgcc agagcgcaac tgaacgatgc
     8281 gacgtttgct gcatcgccgg aaggaaaaac gctgcgaacc gatctgctgc aacgggcaat
     8341 ctacctgaaa caatgatccc aggcagatac gctatacaat gaagcacgcc agcagaacac
     8401 attaagcgcg gcagaacacc gtcagtggtt tgacgtgctt cttgccgggc agctggacga
     8461 tcggatcctg gcactgcaat cacaggggat cttcaccgat cctcagtcat atattactta
     8521 cgcgaccgcg ctggcttatc gtggcgaaaa agcacgcctc cagcattatc tcattgaaaa
     8581 taagccgctg tttaccacgg gcgcacaaga gaaaagttgg ctctatctgt tatctaaata
     8641 cagcgccaac cccgttcagg cattggcgaa ttatacggtg cagtttgccg ataaccgcca
     8701 gtatgttgtt ggcgcgacgc taccggtgct gttaaaagaa ggtcagtacg acgcagcgca
     8761 aaaactgctc gccaccctcc ccgccaatga aatgcttgag gagcgttatg ctgtcagcgt
     8821 ggcgacccgt aacaaggctg aatctctgcg tctggcacga ttgctgtatc agcaagaacc
     8881 gacaaatctt ccccgcctgg atcaactgac ctggcaactg atgcagaacg agcagtcacg
     8941 cgaagctgcc gatttattgc tgcaacgcta tcctttccag ggcgatgcgc gtgtcagcca
     9001 gactttaatg gcgcgactgg cgtctctgct ggaaagtcat ccttacctag caacgccggc
     9061 gaaggtggcg attttatcga aacccttacc gctggcggag caacgtcagt ggcaaagtca
     9121 gttgccgggt attgcagata attgcccggc aatagttcgc ttgctgggcg atatgtcgcc
     9181 ttcctacgat gctgccgcct ggaaccgtct ggcaaagtgt tatcgggaca cgctacccgg
     9241 tgtggcgttg tatgcatggc ttcaggccga acaacgacaa ccgagcgcct ggcaacatcg
     9301 tgcggtagcc tatcaggcgt atcaggttga ggactacgcc accgcactgg cggcctggca
     9361 gaaaatcagt cttcacgaca tgagcaatga tgatctgctc gccgccgcca acaccgctca
     9421 ggcagcggga aatggcgcag ctcgcgatcg ctggcttcaa caggcagaac aacgtgagct
     9481 gggaaacaat gctcaatact ggtggctgca tgcgcgacgt tatatcccag ctcagccaga
     9541 actcgcactg aacgatctca cgcgctcaat caatattgcc ccttctgcca actcttacgt
     9601 tgcgcgggcg acaatttatc gccaacgtca taatgttccg gccgcggtga gtgatttgcg
     9661 cgccgcgctg gaactggaac caaataatag caacacccag gcagcgcttg gttacgcctt
     9721 gtgggatagc ggtgatatcg cacagtcgcg ggaaatgctc gaacaggcgc ataaagggct
     9781 tccggacgat ccggcactga tccgacaact ggcctacgtg aaccagcgtc tggatgacat
     9841 gcctgcgacg cagcactacg cccggctcgt gattgatgac attgataatc aggcataacc
     9901 ccactgaccc cagagcaaaa tcagcaacgc ttcaatttcc gccgtttgca tgaggaggtc
     9961 ggtcgccgct ggacgttcag tttcgattct tccatcggct tgcgttccgg cgcaatgagt
    10021 accgctaaca ataatgtcgg cggtgcagcg cctgggaaaa gctatcgtag ctacggacaa
    10081 ctggaagccg agtaccgcat cggacgcaat atgctgctgg aaggcgacct gctctcagtt
    10141 tatagccgcg tctttgccga taccggagaa aacggggtga tgatgccggt gaaaaatccg
    10201 atgtccggca ccggtctgcg ctggaagccg ctgcgcgatc agatcttttt cctcgccgtc
    10261 gaacagcagt tgccgctgaa cgggcaaaat ggcgcatccg ataccatgct gcgcgccagc
    10321 gcctcattct ttaatggcgg caaatacagc gacgaatggc acccgaacgg ttcaggctgg
    10381 tttgcccaaa acctgtacct cgatgcggcg caatatatcc gccaggatat tcaggcgtgg
    10441 acggcagatt atcgcgtcag ctggcatcag aaggtagcta acggacagac tattgagcct
    10501 tacgctcacg ttcaggacaa cggctatcgt gataaaggca ctcagggcgc gcagcttggc
    10561 ggtgtcgggg tccgctggaa tatttggacc ggcgagacgc actacgacgc ctggccgcac
    10621 aaagtcagtc tcggcgtcga gtatcaacat acctttaagg cgattaatca acgtaacgga
    10681 gagcgcaaca acgcgtttct caccattgga gtgcactggt aaatgcgtaa gttcattttc
    10741 gtattgctga cactgctttt ggtcagccct ttttcctttg cgatgaaagg tattatctgg
    10801 caaccacaaa accgagatag tcaggttacc gatacccagt ggcaggggct gatgagtcag
    10861 ttacgttttc aaggcttcga taccctggtt ttgcaatgga cccgttacgg cgatgcattt
    10921 acccagccag aacagcgcac gttattgttt aagcgtgccg cagctgcgca acagactggc
    10981 ctgaagctga ttgtcgggct gaacgccgat ccggaatttt ttatgcacca gaaacagtcg
    11041 tctgcagcgc tggaaagcta tcttaatcgc ctgctggctg ccgatctcca gcaagccaga
    11101 ttatggagcg ccgcgcctgg cataacgccg gatggctggt acatcagcgc ggaaattgac
    11161 gacctgaact ggcgcagcga agccgcccgt caacctttgc taacatggtt aaacaacgcg
    11221 cagcggctga ttagcgatgt ttcagcaaaa ccggtttata tcagtagttt tttcgccgga
    11281 aacatgtcgc ccgatggcta tcgccaactg ctggaacaag ttaaagcaac cggagttaat
    11341 gtctgggtac aggatggcag cggcgtggat aaacttaccg ctgaacagcg tgaacgttat
    11401 ctacaggcca gcgccgattg ccaaagctcc gccccagcaa gcggcattgt ttatgaactg
    11461 tttgtcgccg gcaaaggcaa aacctttaca gcgaaaccga aaccggacgc agaaattgcc
    11521 tcgctgttag cgaaacgttc ctcttgcggt aaagacatgc tctatttctc tctgcgctat
    11581 ttgcccgtcg cgcacggcat tctcgagtat taaatcccct ccaggtaagt cgggtacgac
    11641 ctggcttacc tctttcgctc tcgtcagaat tccatcaaga ttaattgcta aaaagcatgc
    11701 taaagaaaga agatcgttat cggcgaacgg gccgatcact catgcaggaa gcatagatcc
    11761 tttgagcgga gtttctgata tctgaggagt gcgaaatgca attgagcagc agtgaacctt
    11821 gcgtggtgat cctgaccgaa aaagaggtag aggtaagcgt caataaccat gctacgttta
    11881 cccttccgaa aaactacctg gccgccttcg cgtgcaacaa taacgtcatt gaactctcaa
    11941 cgttaaatca cgtattaatc acccacatca accgtaacat catcaacgat tatctgttgt
    12001 ttttaaataa gaaattaacc tgtgtaaagc cctggtcgcg gctagcaacc ccggttatcg
    12061 cttgtcatag ccgtacaccg gaagtgttcc ggctagccac caaccacagc aagcagcaat
    12121 ccagcaaacc ctgcgaagcg gagttgacgc gcgcattact ttttaccgta ttgtctaact
    12181 ttcttgagca atcgcggttt attgccctac tgatgtatat cttacgcagc agcgtccgcg
    12241 acagcgtctg ccgcattatt caaagcgata ttcagcatta ctggaatctg cgaattgtcg
    12301 ccagttcgct atgtttaagc cccagcctgc tcaaaaagaa attaaaaaac gaaaatacca
    12361 gctatagcca gattgtcaca gagtgtcgta tgcgttacgc cgtacagatg ttattgatgg
    12421 ataacaaaaa tatcactcag gtggcgcaat tatgtggcta tagcagcacg tcgtacttta
    12481 tctctgtttt taaggcgttt tacagcctga caccgttgaa ttatctcgcc aaacagcgac
    12541 aaaaagtgat gtggtgaagg gcaaagcgga aacggataag acgggcataa atgaagaaga
    12601 aatggcgcgc cctgcaggat tcgaacctgc ggcccacgac ttagaaggtc gttgctctat
    12661 ccaactgagc taagggcgcg ttgataccgc aatgcggtgt aatcgcgtga attatacggt
    12721 caacccttgc tgagtcaatg gcttttgatc tggttgctga acaagtgaac gaccgcgtct
    12781 gattttctga tttatttcgc tatagcggca aacaaacgca caccgctgcg cgtctgaatc
    12841 aagaaaaccc gtattttcat gtatcaaagt acaatttacc gccctaacgg aaaattgtcc
    12901 gctcctatga gactggtaac tatgaaacca acgtcggtga tcattatgga tactcatcct
    12961 atcatcagaa tgtctattga agttctgttg caaaaaaaca gtgaattgca gattgtcctg
    13021 aaaacggatg attaccgcat aaccatcgat tatctccgaa cccgtcctgt tgatttaatc
    13081 attatggata tagacttgcc cggaacagac ggttttacct tcctg
//
LOCUS       DAZPCS010000100        13097 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZPCS010000100 DAZPCS010000000
VERSION     DAZPCS010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13097
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.100"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..890)
                     /gene="yafC"
                     /locus_tag="ACHUQ7_002768"
     CDS             complement(<1..890)
                     /gene="yafC"
                     /locus_tag="ACHUQ7_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFR1136100.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAP"
     gene            1131..1931
                     /locus_tag="ACHUQ7_002769"
     CDS             1131..1931
                     /locus_tag="ACHUQ7_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFR1136101.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            2009..2779
                     /locus_tag="ACHUQ7_002770"
     CDS             2009..2779
                     /locus_tag="ACHUQ7_002770"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR1136102.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASALDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2827..4173)
                     /gene="mltD"
                     /locus_tag="ACHUQ7_002771"
     CDS             complement(2827..4173)
                     /gene="mltD"
                     /locus_tag="ACHUQ7_002771"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFR1136103.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDDDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAN
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAQRLANN
                     SDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMPDS"
     gene            complement(4245..5000)
                     /gene="gloB"
                     /locus_tag="ACHUQ7_002772"
     CDS             complement(4245..5000)
                     /gene="gloB"
                     /locus_tag="ACHUQ7_002772"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFR1136104.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINGINEETLLQQPEERFAWLRSKKDRF"
     gene            5034..5756
                     /locus_tag="ACHUQ7_002773"
     CDS             5034..5756
                     /locus_tag="ACHUQ7_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR1136105.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDICLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(5753..6220)
                     /gene="rnhA"
                     /locus_tag="ACHUQ7_002774"
     CDS             complement(5753..6220)
                     /gene="rnhA"
                     /locus_tag="ACHUQ7_002774"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFR1136106.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            6285..7016
                     /gene="dnaQ"
                     /locus_tag="ACHUQ7_002775"
     CDS             6285..7016
                     /gene="dnaQ"
                     /locus_tag="ACHUQ7_002775"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFR1136107.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            7149..7225
                     /locus_tag="ACHUQ7_002776"
     tRNA            7149..7225
                     /locus_tag="ACHUQ7_002776"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7183..7185,aa:Asp,seq:gtc)
     gene            complement(7356..8402)
                     /locus_tag="ACHUQ7_002777"
     CDS             complement(7356..8402)
                     /locus_tag="ACHUQ7_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HFR1136108.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(8416..8975)
                     /gene="tssG"
                     /locus_tag="ACHUQ7_002778"
                     /pseudo
     CDS             complement(8416..8975)
                     /gene="tssG"
                     /locus_tag="ACHUQ7_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601590.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
     gene            complement(8972..10855)
                     /gene="tssF"
                     /locus_tag="ACHUQ7_002779"
     CDS             complement(8972..10855)
                     /gene="tssF"
                     /locus_tag="ACHUQ7_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFR1136109.1"
                     /translation="MDPRLLEYYNRELSYLRETGAEFAARHPKVAARLGMQGTDIADP
                     YVERMVEAFSFLTARTQLKIDAEFPRFTQRLLEVVSPNYVTPTPSMSVAQLHPDTEEG
                     DLAKGFTVPRDTAFFSAIPEGESTACQFRSSQDVTLWPLAIEEARLTAAPPDMPALHR
                     YLPANIHVAGALRITLRTFGELTFSQLAGLDRLPFYLCGEERTASHLLELLHTSAIAP
                     LAGIPGHFDGALDVNLQQPVMYEGLEPDQGLLPLAWNVFHGHNLLHEYFACPERFYFF
                     TPTGLSAGLQKIDGGVAEIVILLNRLPPDWLIHQTNAAQFSLFCTPVINLFPRVTARI
                     DVTHSTTEQHLVVDRTHPLDYEVFSVQEVEGLETDTTRKMAFRPLYHTRNNDEGNHGR
                     YFSLRREPRRLSENARRYGTRTPYTGSEVFLSLVDQYEAPYPENLRHITITAMVTNRD
                     LPCLIARNGRDDLTVDAAIPVAGVGLIRPPRSPQPPMAEREMAWRLIRQLSFNYLPLA
                     DLDHRTGGQALRDLLNLFIPAHDSPQSRQVRSLIGCKTTPVTRRLPGSGLLVYGRGVS
                     CELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMQRGKIMTWPVRAGQR
                     GSV"
     gene            complement(10871..11365)
                     /gene="tssE"
                     /locus_tag="ACHUQ7_002780"
     CDS             complement(10871..11365)
                     /gene="tssE"
                     /locus_tag="ACHUQ7_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFR1136110.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(11362..12192)
                     /locus_tag="ACHUQ7_002781"
                     /pseudo
     CDS             complement(11362..12192)
                     /locus_tag="ACHUQ7_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system accessory protein TagJ"
     gene            complement(12179..13093)
                     /gene="tagK"
                     /locus_tag="ACHUQ7_002782"
                     /pseudo
     CDS             complement(12179..13093)
                     /gene="tagK"
                     /locus_tag="ACHUQ7_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_137593242.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
BASE COUNT         3007 a         3393 c         3484 g         3213 t
ORIGIN      
        1 ggagctgttt ttacatgctc gctaaggaaa tcgataaaag cccggatgcg cgtacttacc
       61 gcacggtcgc tgtaatagac ggcactgaat ggcatttcca ctggcaacac tttatctgcc
      121 attaactcca ccaattctcc gcgagcgatt tctttgtcga tcatgtagtc ggacaaacac
      181 gcaatcccgt tgccactcag gcaaagctgt ttcagtgttt ccccactatt ggatgacaaa
      241 ccgtacttca cctcatgtaa ttgtccatca ctacaggcta tcggccaggt attgagggaa
      301 gcgggttcag tgaatcccag gcaaacatgt tgctttaaat cgtcgatcgt ttctggcttc
      361 ccgtagcggg aaatataatc gggggaggcg ataatttttc gataactgtt aaataacggc
      421 ctggcacgta agctggagtc cgttaacgta ccagcgcgta tcgcgacatc cacttttctt
      481 tcgatcaaat taataatcgt ttcggaggag actagcgata aagtgacttc cggatagcgt
      541 tcacggaaag gcttaattaa cggcatcaga aagtgcagca ccactggagt tgcggcatcg
      601 atccgtaaca gtccacgcgg tgtattacgc gtctccataa tttctgattc tgccgctgcc
      661 atctcctgca aaattgactg tacgcgacga aaataacgct cgccttcttc cgtcaggcta
      721 agttgccgcg tggtccgatt aagcaggcta acgccaagtt tcatttccag ctttttcacc
      781 gcccggctta ccgctgagtt tgcttgccct aattgttccg ctgcccggct aaagctgccg
      841 ctttcgacga ccgaaacaaa aatggcgagt tcttccgacg tggctttcat ttttgctcct
      901 gttgcaaaat agatgagata ttttgaattt atttgtcatt aaaccatcag gatgcgtgat
      961 atgtcatccg atttaatgtt ctcaataatg agcaaatttc tgacaggtgt aagcacttgc
     1021 ttacataaca atatacaatt gctcgttgaa agagtgagct aaaatcccta taacagtaga
     1081 accctcccga gtgcggaagg gttgacgtaa tagaggtttc aaagtcaaaa gtgcgaaaaa
     1141 acacctatgc catgcgctat gttgccggac aacctgcgga aaggatctta ccgccggggt
     1201 cttttgcgag catcggccag gcattaccac ctggagaacc gttaagtacc gaagagcgta
     1261 ttcggatcct ggtgtggaac atatacaaac agcaacgcgc tgaatggttg tcggtattaa
     1321 agaactacgg caaagatgca catctggtgt tattgcagga agcgcagaca acgccagagt
     1381 tagtacagtt tgcgaccgct aactatcttg ccgccgatca ggtgcccgct ttcgtgctgc
     1441 cacaacatcc ttccggtgta atgacccttt cggcggcgca tccagtgtat tgctgcccgt
     1501 tacgtgaacg agaacccatt ttgcgtctgg cgaagtcggc actggtgacg gtctatccat
     1561 tacctgacac ccgcctgttg atggtggtta atatacacgc cgtcaacttc agtctgggcg
     1621 tggatgtcta tagtaagcag ttacttccta ttggtgatca gatagctcac cacagcggcc
     1681 cggtcattat ggcgggagat ttcaatgcct ggagccgtag aaggatgaac gcgttatatc
     1741 gctttgcgcg ggaaatgtcg ctgcgccagg tgcgttttac cgatgatcag cgccgtcggg
     1801 cgtttggccg cccgctcgat tttgttttct accgtggtct gaacgtcagc gaagcttctg
     1861 tattggttac gcgcgcttcc gatcacaatc cgctactcgt tgaattcagt cccggcaagc
     1921 ctgataaata aggtatgtca ggtctgccac agggcagacc aacgtttggc gctgtccttt
     1981 tatactcaaa caacaaaagg acagcgcaat gacaacacaa tcccaccatg accacgtaga
     2041 aaagcagttt agctctcaag cctgcgaata tctgaccagt accgtacatg catccggacg
     2101 agatttacag cgcctggcgg tgcgtctggc tgattatcct ggtgcaagtg cacttgatat
     2161 gggctgcgga gcagggcatg ccagctttgt tgctgcgcaa aacgtgagcg cggtggtggc
     2221 gtatgactta tctgcccaaa tgctggatgt cgtggcacaa gctgccgaag cccggcaact
     2281 gaaaaatatc accacccgcc agggatatgc cgaaagtctg ccatttgccg ataacgcatt
     2341 tgatattgtt atcagccgtt attctgccca tcactggcat gatgttggtg cagcactgcg
     2401 tgaagtgaac cggatattga aacctggcgg taggatgatt gtgatggacg taatgtctcc
     2461 gggtcatcca gtgcgcgaca tctggttaca gacggtagaa gcattacgcg atacctctca
     2521 cgtacgaaac tacgccagcg gtgagtggtt gaggttaatc aatgaagcca atctgatagt
     2581 tgataattta attacagata agttaccgct ggaattttgt tcatgggtcg cgagaatgcg
     2641 tacgccagaa gcgttagtag acgctattcg catttaccaa cagagcgcat cgacagaggt
     2701 gagaacgtat tttgccttgc agaatgacgg ctctttcacc agtgatatca tcatggtaga
     2761 agcacataaa gcggcataaa taaaaaaggc accgggggaa tcggtacctt tttattatct
     2821 ggtttgtcag gaatctggca tgttgttgtt tttcacaaac aacgtcagct tgtcgcctgg
     2881 ttgcagattc gcagtgtcgc tgttccagcg catcacatct ttgatgttca cgccgtggcg
     2941 tttagcaatg cttgaaagcg aatcgccttt gcgcacacga tacgtaatgc tatcgctgtt
     3001 gtttgccagt cgctgtgcac caatcgtcaa actttggcct ggcttcagct tagatccgcg
     3061 cagtttgttc cactgctgca aatctttggt gcttacgccg agacgtgaag cgatgcttga
     3121 aagcgtgtcg ccagagcgta cggtgtaaac acggctgtta agcggcgtat tgttggcaac
     3181 cagcgtcgac tgtacagcag caatttcgcc tgaagcgaga gattcacgca gttgatctgc
     3241 atgcttcttt ggcaccatca cgtactgcgg cccacttgcg cccagcgtgg agcctttcac
     3301 gccagcgttg aatgtcttca gcttgctgac ggaaatcccc gccatatctg caaccttcgc
     3361 catttcaacc gggctactca ggtgcacacg cgccagagca cggctttcat cggtcgttgg
     3421 cagacgtacg ccataacgct tgctgttttt gagaatatca ctcaatgcca gcattttagg
     3481 cacgtactgc ttcgtttcct gcggcaacgg taacgaccag aagtccgtgg atttcccacg
     3541 cgctttgttc gttttaattg ccttcatgac ccgaccttcg ccgctgttat aagccgctac
     3601 ggtcagaagc cagtcgtcgt caaacatttt gttcagacgc tgcatcatat tcagcgcggc
     3661 agttgttgaa gcaacaacat cgcgacgcgc gtcataattg cgggtctgtt tcaaaccata
     3721 attgcgcccc gtgctcggaa tgatctgcca gatgcctgcg gcattggcgc cagacgttgc
     3781 gtgaggatca aaagcgctct ccactatggg tagtagtacc agttccatag gcatgttacg
     3841 ttttttaact tgccctgcta tccagtacat atacggctct gcccgtaaag ttacatcgtg
     3901 gagatagctc ttattgcgta aatatttctg tttctgttcg cgaatccggt cattttccgg
     3961 aattcccatc tttagctcgt cgccaatgaa agcccacaag tcaccatctg gcgcgataga
     4021 cgtcccatcg tccatccatc gtgcctgact tgtaaacttt gctgcttccc cttgaccagc
     4081 tgcagaaagg ctctgtgcgt gctgttgaac gttgccggta ctctggcaac ccacgagcag
     4141 gacagaggcg agtaatatcg cttttgcctt catgtgtgtg tcaatagttg cttaaaagac
     4201 gaccgatcat aacggcgaac ggagccgatg acaagaaagt tttatcagaa cctatctttc
     4261 tttgacctta accatgcaaa acgctcttca ggttgttgca ataatgtttc ttcattaatt
     4321 ccattaatta aatcaatatc ttccgttctt aaaaaaacat taatttgccg ctcatttttc
     4381 agaattacgg gtagtgttat ttgatttttt gcccgtaact ccttaacttt acgataataa
     4441 tcatttatgg acaaatcgtg cggaaaaata ctcaaagcaa acttcatatt tgataacgta
     4501 tattcatgag cacaacatac caatgtatcg tcaggtaacg cacttagctt tttaagtgat
     4561 tgatacattt gtaaagctgt cccttcaaac aaccgaccac acccaccaga aaacagcgtg
     4621 tcgccgcaaa atagataagg tttactgaag taacagatat gtcctaaagt gtgacccggt
     4681 gtggtaatta cactaaattc atgccctaaa acgaaggcag tttcgccatc tttgactacc
     4741 tgtgttgttc ccttatcttg tgtctcttgt ggaccataca ctacaatttg cggaaacttt
     4801 tccaccagtt cttttacgcc gccaacgtga tcgtggtgat ggtgggttag aaatatggcc
     4861 tccggttgcc agttattggc ggtaatggcg tttaatactg gctctgcgtc tccgggatcg
     4921 acaatcaggc agcgacctgc ctcatcattc aaaacccaga tgtaattgtc atcaaaggcg
     4981 ggaatactgt taagattcat agattacctc tcagtgtaaa acggaaggtt gtgatgaaac
     5041 cggcaagagt ccctcaaact gtcgtggctc ctgattgctg gggcgatttg ccctggggag
     5101 agctttatcg caaggcgctg gagcgccagc tcaacccgtg gttcactaaa atgtatggtt
     5161 ttcatctgct taagattggc aatttaagcg cagaaatcaa ttgcgaagcg tgcgcggttt
     5221 ctcatcaagt gaatgtttct gcgcaaggaa tgcccgtcca ggtgcaggcg gatccacttc
     5281 atcttccttt tgccgataaa tccgttgata tttgtctact ggcacatacc ttgccgtggt
     5341 gcaccgatcc gcatcgttta ttacgtgaag ccgatcgggt actgattgat gatggctggc
     5401 tggtgattag tggcttcaac cccatcagtt taatgggatt acgcaaactt gtgccggtat
     5461 tgcgcaaaac ctcgccctat aatagccgga tgtttactct gatgcggcaa ctggactggc
     5521 tctctttgtt gaattttgaa gtgctgcacg ccagccgttt ccacgttctc ccgtggaaca
     5581 aacacggagg aaaactattg aatgcgcata ttcctgcgct tggttgctta caacttattg
     5641 ttgcccggaa acggactatt cctttaacgc taaatccgat gaaacagagt aaaaacaagc
     5701 cacgaattcg ccaggcggtt ggagccaccc ggcaatgtcg taaaccacag gcttaaactt
     5761 cgacttggta gcccgtatct tccagtgtgg gattcatcgc cgcggcacga gccagttcat
     5821 cacagcgttc gttttccggg tgtccggcat ggcctttaac ccattcccat ttgatttgat
     5881 gctgccccaa tgcagcatca agacgttgcc agagatcgac attttttact ggttttttgt
     5941 ctgctgtttt ccagccacgt tttttccagt tatggatcca ctgggtgata ccctggcgga
     6001 catactggct gtcggtactc aaaatgactt cgcaatgttc ttttaacgcc tccagcgcga
     6061 caatagcggc catcaactcc atacggttgt tggtggtgcg ggtgtagcct gcgctaaagg
     6121 ttttctcgcg tccgcgatag cgtaaaatag cgccgtaacc cccaggtcca ggattaccca
     6181 gacacgaacc atcggtgaaa atttctacct gtttaagcat ctctggtaga cttcctgtaa
     6241 ttgaatcgaa ctgtaaaacg acaagtctga cataaatgac cgctatgagc actgcaatta
     6301 cacgccagat cgttctcgat accgaaacca ccggtatgaa ccagattggt gcacactatg
     6361 aaggccacaa gatcattgag attggtgctg ttgaagtagt gaaccgtcgc ctgacgggca
     6421 ataacttcca tgtttatctc aaacccgatc ggctggtgga tccggaagcc tttggcgtac
     6481 atggtattgc tgatgaattt ttgctcgata agcccacgtt tgccgaagta gccgatgagt
     6541 ttatggacta tatccgcggc gcggagttgg tgatccataa cgcagcgttc gatataggct
     6601 ttatggacta cgagttttcg ttgcttaagc gcgatattcc gaagaccaat accttctgta
     6661 aggtcaccga tagccttgcg gtggcgagga aaatgtttcc cggtaaacgc aacagcctcg
     6721 atgcgctatg tgcccgctac gaaatagata acagtaaacg aacgctgcac ggggcattac
     6781 tcgatgccca gatccttgcg gaagtttatc tggcgatgac cggtggtcaa acgtcgatgg
     6841 cttttgcgat ggaaggagag acacagcagc aacaaggtga agcaacaatt cagcgcattg
     6901 tacgtcaggc aagtaagtta cgcgttgttt ttgcgacaga tgaagagctg gcagcacatg
     6961 aagcccgtct cgatctggtg cagaaaaaag gcggaagctg cctctggcgg gcataaatac
     7021 atgtaaaagg cgctaaaaat agcgacttgg gcgatttttg cagcaaacga ttcaaaagat
     7081 gagaaaaatc gttgacgaag gtcgaggcaa tccgtaatat tcgcctcgtt cccaacggaa
     7141 cacaacgcgg agcggtagtt cagtcggtta gaatacctgc ctgtcacgca gggggtcgcg
     7201 ggttcgagtc ccgtccgttc cgccaatatt cagaaagccc gaatcagtaa tgattcgggc
     7261 tttcgtcgtt ttggtgaagt gagaattttt cactccgaag taaaacggat atgatttaaa
     7321 aaatggccag acattataaa ttctggccac ttctgttact ccagcgcatc ccatttctcc
     7381 agcagttcta ctggaatttc atttattacc tccgagacgc gttttccgac caggcgttct
     7441 gcctggcgca gcaaaggaat ggtcgggctg ctcggttcgc tgtgctcaaa ccagcgacgg
     7501 atgacacgca ggcgttccag cgcgtcgttt cggtcgcgga tctgccgggg ttctgcgccg
     7561 gtcatcggtg atatttcctc cgcgatatgc accggtagcg caatctgctc tgcctgcatc
     7621 tgttcagtat gaacgatggt ggcgttttcc ggctgctcag cctgtggttg tggcaggatc
     7681 tccggcgtgg tgctctgcac tgctcccggt aacagggcca gcagttgcgt cagacgggag
     7741 aagtccggtg cctggtcatt cagcgtttca cgcgcatggc gttgcagccg ctcagtggtc
     7801 tctgctgcct ggcgaaaggc atccagcggc agtgtgccgc gcgcttccag gtctgccagt
     7861 tgctggcgta ccgattctgg tgccagcgca tccgccgggc gtaatgcaga tagcgaacgc
     7921 tccacatccc gcacctgaag gcgcatggca gcattatttg acagtgtaat gccacgaata
     7981 tccgccatta caccttcgtg gtccagcaac gccgccagcg cgttactgcg tgccaccgca
     8041 gcatcttcgg cggtgatatc ttcagtcgcg agcagttgag gatggatggc gtccgggtac
     8101 gtggagcaca acgtttccag cagcgtgagc gcctccgaca gtcccctggc accggcctgc
     8161 tggatacggc agcgcagcag gacaaccaga atgcggatat ctttgctgcg catcagcaga
     8221 cgatgggcgt cccgctcaag ctccgcccag ttaatgtttt ccggcgtact gacaaaatcg
     8281 ccatattgcg cttcttcacg cggtgcggcg cgggtgaaca gcagcaggta ttccggatcg
     8341 tactccagat ccgggccgca ggggcattcc gccgaaattg ggtcagacag gaatatttcc
     8401 atttaattat cctcagtgcg ccagcgtggt ggccagaccg tccggattgc tggactcacg
     8461 caccagatgg gccgatgccg ccagacgagc gtgatctggc aacgggctgt cagcgctctc
     8521 ccgcaaatcc tgcccgctga ggctggcgac ataaaaggca aagcggtcat gttgcggttt
     8581 atccagaccg cccccagcgg actgggcgct gcgccatgcc tggtaaaact gcgtcagcca
     8641 gcggtgatga aaaagatcag aaaaacgggt cagcgtcggc tttcagtccg gttacgcgcc
     8701 agttcggtgt agtgcagcgg cagtggaccg ttcggccccc acagcacgag gctgtacaga
     8761 ctgatgtgca ggcgcccttc ctgccagccc acactggcga tttctcgtgg cgcaaaggtc
     8821 agcgcgggcg tttgtccgag gcgaaatttt tccatccgtg gttgccaggt gttgcccgcc
     8881 ggaacggtac acaactgcac atccacgcgg cgcatcaggt tcagaaaacc gtaacgccag
     8941 gggggggcct gcgcctgagt gaaatcgttg ctcatacgct tccccgctgt cccgccctga
     9001 caggccaggt cataattttg ccgcgctgca tgctgtgcag cgtcatctgc gagaaggtat
     9061 taatggaaac atggcgggcg atatagtgtt ccagcaccag gccgaacaga tacggactga
     9121 tgccggaaaa accttcttca tccaccgtca gttcacaact gacgccacgc ccatagacca
     9181 gtagtccgga accgggcaga cggcgggtga ccggggtcgt tttacaacca atcaggctgc
     9241 gcacctggcg cgattgcgga ctgtcatgag caggaataaa caggttcagc agatctcgca
     9301 acgcctggcc gccggtacgg tgatccagat cggccagcgg cagataatta aaagataact
     9361 ggcggatcaa ccgccatgcc atctcacgtt cagccatcgg gggctgcggc gagcgtggcg
     9421 ggcggataag ccctacgccc gccaccggga tggctgcatc cacggttaga tcgtcccggc
     9481 cattgcgggc aataagacag ggcagatcgc ggttagtgac catggccgtg atggtgatgt
     9541 ggcgcagatt ttccgggtac ggcgcttcat actggtcaac cagcgacagg aacacttccg
     9601 agcccgtata gggggtacgg gtgccgtaac ggcgggcgtt ttctgataag cggcggggct
     9661 cccggcgcag ggaaaaataa cgtccgtgat tgccttcatc attattgcgg gtgtggtaga
     9721 gcgggcgaaa ggccatcttc cgtgtggtgt cggtttccag cccttccact tcctggaccg
     9781 agaacacctc ataatccagc ggatgggttc tgtccaccac cagatgctgt tccgtcgtgc
     9841 tgtgcgtgac atcgatccgg gcggtgacgc ggggaaacag gttgataacc ggggtacaga
     9901 acaggctgaa ctgtgccgca tttgtctggt ggatcagcca gtccggcggc aggcggttca
     9961 gcaggataac aatctccgcc acgccaccgt caattttctg taatccggca gacaggcctg
    10021 tcggggtgaa aaaatagaag cgttccggac aggcaaaata ttcatgcagc aggttatgac
    10081 cgtgaaacac gttccacgcc agcggcagca gcccctgatc cggctccagg ccttcgtaca
    10141 taacgggctg ttgcagattc acatccagcg cgccgtcaaa atgccccgga atgccagcca
    10201 ggggcgcgat ggcgctggta tgtagaagtt ctaacaggtg tgaggccgta cgctcctcgc
    10261 cgcagagata aaacggcagc ctgtccagtc cggcaagctg actgaacgtt agttcaccaa
    10321 aggtgcgcag ggtaatacgc agcgccccgg cgacgtgaat gttggccggc aggtagcggt
    10381 gcagggcagg catatccggt ggcgcggcgg tcagtcgcgc ctcttcgatg gcgagcggcc
    10441 acagcgttac atcctggctg ctgcgaaact ggcaggcagt gctttcgcct tccggaatgg
    10501 cggagaaaaa ggctgtgtcg cgcgggacgg taaatccttt cgccagatcg ccttcttccg
    10561 tatcgggatg caactgcgcc acgctcattg agggcgtggg tgtgacgtaa ttggggctga
    10621 ccacttccag caggcgctgg gtgaaacggg ggaattcagc gtcaattttt agctgcgtgc
    10681 gggcggtcag gaagctgaag gcctcgacca ttcgctctac atacggatcg gcaatatccg
    10741 ttccctgcat gcccagccgg gcggcgactt tgggatgacg ggcggcaaat tcggctcccg
    10801 tttcccgcag atagctcagt tcgcggttgt agtattccag tagccgtgga tccatcggtc
    10861 tctctctgaa ttaaaaaaac gtaatgcgac tctgctccat atccagcgcg ctacgtaccc
    10921 ggaatgcagt cggatacggc tgcgtgagga tctcgccgcg aatttcaaac tgtaatgtgt
    10981 tatggccact ctggcggcgc tcatcctgaa gtgggatgat ttgcagagtg gacgctttca
    11041 gccggggttc aaaccgggtg atggttcgca aaatggccct gcggatatcg tcccacttgt
    11101 gctcatacag aaagctgccc gccagtggcg gcaggccgta gttaagtacg gacgctgccg
    11161 cgtacggata acgtgcagca tcaatatcat cttcatggct gacggtgttg agcagaaatg
    11221 acaaatcccg gcggatgatc tccttaatct gaaccggact gacgctgata ccgtggtcgc
    11281 gtttctggtt tggggcgtcg tcacacaggc ggtcaaacag cgtgggcagc aggtgattgc
    11341 ctggacgctg gcgggaagcg ctcacgcgtc gcttccttca gcggtgttaa aggtacaggt
    11401 ggacaaatcc agcaggctga tatcgccgtg accggtgagc cacaccctct gacccagcgc
    11461 ctgtaccagt gtctctcccg gaccgtcctg ccagaccgtt tcccggcaca gacgcaggga
    11521 gtcggcagcc ttttcagagc cgctgtagcg gggaaacagc taggcactgt gtgtatcgcc
    11581 attgttcagg gtgacgttga cgggtttcca gatcaaatcg atcagatgcg caggccgcgg
    11641 cgatttcagc gaacagattt gtgaaaacgg cagccagata tatgctccgc ccgcgatcag
    11701 ttcgagtacc ggaccggtac gggagtcgct gtcgctaatc cagtcaaatg cgccgccatt
    11761 ccactgcccg gaagtgtctg ttatggcctc aagcgcgcgg ctgcggtgag catcagcttc
    11821 ctgcgcttca ccacgggcgt tacaggcgag ggcggtcagc agatcctcca tccaggcggg
    11881 cggcgggaga ataacaccgg gccgctgttc gccctgaaag caggcgtggc ggtatatctc
    11941 acagcggatc agctcgccaa atacctgcgc ttcgcgagta taatttgcgt ccatccgggc
    12001 gcatagctgg atctgttgca gtgcccgcga ccagtcgcct gccacgcaca ataactgaaa
    12061 caggctgtgg cggcgggagg cctgcgctgg gtgcatcctg acctgttgct ctgccatctg
    12121 aatgccctcc accacggaat aatcccgcag cagcgccgac aaggtaatcg gtagctcgtt
    12181 attgtttgtc atgatatatc tctccatgtt gtgccgggtt tatccggtaa tggctgtcaa
    12241 tgccaatgat gcgatgctct ttccgtgtca gatctggcag tacggtgtta tgcgctgttt
    12301 ccggggcgta ttccggggaa agtaaatgca ggatatcagg gggagtcttt tcgacaggcc
    12361 agatagtttc atcttcgcca gtggcatcca gtacattgag aatagcgtca atgcttaacc
    12421 caccagtgac catatcctgc aatgtctcgg cttcggtgac caggtcacgc aatgaatcca
    12481 gatcctgcga ggagggctct tcgttaaaag gaaaaggatc agcatggcga ttaagatctt
    12541 gttcgggaga taacaacgcc tcgcggtatt cctgataaag tcgggtcagg gcatttcctt
    12601 tttccgcacc ttctccggtg gaggaggctc catgaacagg aatgatatcg aatggatttt
    12661 gtgatgtcag atgctgctgc ctgaaccaat ccagatccag atattcggtt attgcttccg
    12721 acgtgatggc agtctgcgat atcgttttta ctgtgctcag gttcggagat gatggattgc
    12781 catgtacaag gcactatgaa gataaccccc attctatcag gtcgccttca ttcagacgca
    12841 tacggtgatg aggttccaca atctggtcgt ttaccgtaca gcaatattcg gtagactgat
    12901 tgataatcca ccatgcttct tcgtggcggg tcagagtgag ggcaatcttt acatcatcat
    12961 tcaccttaaa aacaggaatc cagggagagg taacggtgaa gataatttgt gttccttcag
    13021 tcgcgtaaat tgtgttatcg gcgatcccgt gcgcctgtat tttttggagt ccccacgtaa
    13081 tatttggttt cattgtg
//
LOCUS       DAZPCS010000101        13048 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZPCS010000101 DAZPCS010000000
VERSION     DAZPCS010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13048
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.101"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            151..801
                     /gene="csgD"
                     /locus_tag="ACHUQ7_002783"
     CDS             151..801
                     /gene="csgD"
                     /locus_tag="ACHUQ7_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFR1136111.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            806..1195
                     /gene="csgE"
                     /locus_tag="ACHUQ7_002784"
     CDS             806..1195
                     /gene="csgE"
                     /locus_tag="ACHUQ7_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFR1136112.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDSEKTVVFAL
                     IQTEEALNRRQINQALLSTDDLAHDEF"
     gene            1220..1636
                     /gene="csgF"
                     /locus_tag="ACHUQ7_002785"
     CDS             1220..1636
                     /gene="csgF"
                     /locus_tag="ACHUQ7_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFR1136113.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1663..2438
                     /locus_tag="ACHUQ7_002786"
                     /pseudo
     CDS             1663..2438
                     /locus_tag="ACHUQ7_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
     gene            complement(2502..2993)
                     /locus_tag="ACHUQ7_002787"
     CDS             complement(2502..2993)
                     /locus_tag="ACHUQ7_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFR1136114.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(3095..3649)
                     /gene="ycdY"
                     /locus_tag="ACHUQ7_002788"
     CDS             complement(3095..3649)
                     /gene="ycdY"
                     /locus_tag="ACHUQ7_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFR1136115.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMLL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3673..4410)
                     /gene="ycdX"
                     /locus_tag="ACHUQ7_002789"
     CDS             complement(3673..4410)
                     /gene="ycdX"
                     /locus_tag="ACHUQ7_002789"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFR1136116.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMISTIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPLER
                     ILNVSPCRLLNFLESRGMAPIAEFADL"
     gene            complement(4465..5403)
                     /gene="ghrA"
                     /locus_tag="ACHUQ7_002790"
                     /pseudo
     CDS             complement(4465..5403)
                     /gene="ghrA"
                     /locus_tag="ACHUQ7_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
     gene            5638..5725
                     /locus_tag="ACHUQ7_002791"
     tRNA            5638..5725
                     /locus_tag="ACHUQ7_002791"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5672..5674,aa:Ser,seq:gga)
     gene            complement(5875..6156)
                     /locus_tag="ACHUQ7_002792"
                     /pseudo
     CDS             complement(5875..6156)
                     /locus_tag="ACHUQ7_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(6438..7442)
                     /locus_tag="ACHUQ7_002793"
     CDS             complement(6438..7442)
                     /locus_tag="ACHUQ7_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSfl8 family transposase"
                     /protein_id="HFR1136117.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARCISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTETQQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPIGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHYFYLVEQIAELESELSQSINADDTAQRIMTIPGV
                     GPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLGISKRGDKNLRRLL
                     VQCARSFIMRLERKHGKLAEWVREQLNKKHSNVVTCALANKLARIAWAITTQKKEYQA
                     "
     gene            complement(7514..8074)
                     /locus_tag="ACHUQ7_002794"
                     /pseudo
     CDS             complement(7514..8074)
                     /locus_tag="ACHUQ7_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(8159..8356)
                     /locus_tag="ACHUQ7_002795"
     CDS             complement(8159..8356)
                     /locus_tag="ACHUQ7_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFR1136118.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(8368..8859)
                     /locus_tag="ACHUQ7_002796"
     CDS             complement(8368..8859)
                     /locus_tag="ACHUQ7_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFR1136119.1"
                     /translation="MMKLALTLEADSVNVQALNIGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            complement(8856..9230)
                     /locus_tag="ACHUQ7_002797"
     CDS             complement(8856..9230)
                     /locus_tag="ACHUQ7_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFR1136120.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFV
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(9277..9651)
                     /locus_tag="ACHUQ7_002798"
     CDS             complement(9277..9651)
                     /locus_tag="ACHUQ7_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFR1136121.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            complement(9814..10035)
                     /locus_tag="ACHUQ7_002799"
     CDS             complement(9814..10035)
                     /locus_tag="ACHUQ7_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFR1136122.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(10098..10574)
                     /locus_tag="ACHUQ7_002800"
     CDS             complement(10098..10574)
                     /locus_tag="ACHUQ7_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFR1136123.1"
                     /translation="MQQLSFLPGNMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(10590..11063)
                     /locus_tag="ACHUQ7_002801"
     CDS             complement(10590..11063)
                     /locus_tag="ACHUQ7_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFR1136124.1"
                     /translation="MKTLSQNTTASACAPETGLQQLVATPVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            complement(11405..12226)
                     /locus_tag="ACHUQ7_002802"
     CDS             complement(11405..12226)
                     /locus_tag="ACHUQ7_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFR1136125.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKR
                     YAYTPTITVLESLQREGFQPFFACQTRVRDPGRREYTKHMLRLRRAGQITGQHVPEII
                     LLNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLESLR"
     gene            complement(12326..12499)
                     /locus_tag="ACHUQ7_002803"
     CDS             complement(12326..12499)
                     /locus_tag="ACHUQ7_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFR1136126.1"
                     /translation="MSRLLPEVPFSREQAVVITTAYRNVFIDDDQGTHFRRVIRNAET
                     DAGRFASDGLLRQ"
     gene            complement(12619..>13048)
                     /locus_tag="ACHUQ7_002804"
     CDS             complement(12619..>13048)
                     /locus_tag="ACHUQ7_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1136127.1"
                     /translation="SPDTNAATVNQTLKCDSKARADQPGACPTTYELYEGDATYRAAI
                     DKALNPVGLNGMFGEDGYMDGPDGGAYPVNINGTTWVEGGGCKAHACGWDYIVTLYNP
                     KTHKVVGYYYNIDPGYLIWFGETGVHEFAYLVRDYVNKTN"
BASE COUNT         3296 a         3231 c         3209 g         3312 t
ORIGIN      
        1 aatcagatgt aatccattag ttttatattt tacccattta gggctgattt attactacac
       61 acagcagtgc aacatctgtc agtacttctg gtgcttctat tttagaggca gctgtcaggt
      121 gtgcgatcaa taaaaaagtg gggtttcatc atgtttaatg aagtccatag tattcatggt
      181 catacattat tgttgatcac taaaccttct ttgcaggcga cagctctctt gcagcacctt
      241 aaacaatcgc tagcaattac aggaaaatta cataatattc aacgttctct ggacgatatc
      301 tcttcaggct ctattattct tctggatatg atggaagcgg ataaaaagct tatccattat
      361 tggcaggata ctttgagcag gaaaaacaac aatatcaaaa tattgttgct aaatacgcct
      421 gaagattacc cgtaccgcga cattgaaaac tggcctcata tcaacggcgt tttttatgcc
      481 atggaggatc aagaacgtgt tgtcaatggg ttgcaaggcg tcctgcgtgg cgaatgctac
      541 tttacgcaaa agcttgccag ttacctgatt acacattctg gtaactatcg ttataacagc
      601 acggaatcag ccctccttac tcatcgggaa aaagagatcc tgaataagct gcgtatcggc
      661 gcgtctaata acgagatcgc tcgttcgttg ttcatcagcg aaaatacggt taaaacgcat
      721 ctttataatc ttttcaagaa gatagccgta aaaaaccgga cacaagcggt ttcctgggca
      781 aacgataacc tcaggcgata aagccatgaa acgttattta cgctggattg tggcggcaga
      841 atttctgttc gccgcaggga atcttcacgc cgttgaggta gaagtcccgg gattgttaac
      901 tgaccatact gtttcatcta ttggccatga tttttaccga gcctttagtg ataaatggga
      961 aagtgactat acgggtaact taacgattaa tgaaaggccc agtgcacgat ggggaagctg
     1021 gatcacaata acggtcaatc aggatgttat tttccagact tttttattcc cgttgaaaag
     1081 agactccgag aaaactgtcg tctttgcact gattcaaact gaagaagcac taaatcgtcg
     1141 ccagataaat caggcgttat taagtacgga cgatttggcg catgatgaat tctaaataaa
     1201 aaattgttcg gaggctgcaa tgcgtgtcaa acatgcagta gttctactca tgcttatttc
     1261 gccattaagt tgggctggaa ccatgacttt ccagttccgt aatccaaact ttggtggtaa
     1321 cccaaataat ggcgcttttt tattaaatag cgctcaggcc caaaactctt ataaagatcc
     1381 gagctataac gatgactttg gtattgaaac accctcagcg ttagataact ttactcaggc
     1441 catccagtca caaattttag gtgggctact gtcgaatatt aataccggta aaccgggccg
     1501 catggtgacc aacgattata ttgttgatat tgctaaccgc gatggtcaat tgcagttgaa
     1561 cgtgacagat cgtaaaaccg gacaaacctc gaccatccag gtttcgggtt tacaaaataa
     1621 ctcaaccgat ttttaagccc cagcttcata aggaaaataa tcatgcagcg cttatttctt
     1681 ttggttgccg tcatgttact gagcggatgc ttaaccgccc cgcctaaaga agccgccaga
     1741 ccgacattaa tgcctcgtgc tcagagctac aaagatttga cccatctgcc agcgccgacg
     1801 ggtaagatct ttgtttcggt atacaacatt caggacgaaa ccgggcaatt taaaccctac
     1861 ccggcaagta acttctccac tgctgttccg caaagcgcca gggcttacaa aacctgctta
     1921 acgagcgcaa ggttattcgt gcggcacaag aaaacggcac ggttgccatt aataaccgaa
     1981 tcccgctgca atctttaacg gcggcaaata ttatggttga aggttcgatt atcggttatg
     2041 aaagcaacgt taaatcgggc ggtgttgggg caagatattt tgggatcggt gcagacacgc
     2101 aataccagct cgatcagatt gccgtgaacc tgcgtgtcgt caatgtgagt accggtgaga
     2161 tcctctcctc ggtgaacacc agtaagacga tactttccta tgaagttcag gccggggttt
     2221 tccgctttat tgactaccag cgcttgctgg aaggggaagt gggttacacc tcaaacgaac
     2281 ccgttatgct gtgtctgatg tcggctatcg aaacaggggt cattttcctg attaatgatg
     2341 gtatcgaccg tggtctgtgg gatttgtaaa ataaagcaga acggcagaat gacattctgg
     2401 tgaaataccg ccatatgtcg gttccaccgg aatcctgaca gataaaaatg ccggaattaa
     2461 taatccggca tttactgttt aagcgaaagc gtgagtgccc ctcacgcttt atttttgatt
     2521 tcctgctcac ggtgagccgt ctttgcacta cgcgccgcca gccacaggcc gctgtttttc
     2581 attgcgtaac caaataccag cccaagcgcc agcgaaggta acaccagttt ccagtcaccc
     2641 tgcccggcaa acgtcgcgca tgcgcctata aacgtccccg gaacaaatga aagcagcagc
     2701 tgtttggcct gaatacacat cagaaacgcg acaataccgg ttatgacata accgagaatt
     2761 tccagatgtg gtgccagtgc actaccgtaa ataatgacca tcgcccacac cacgccacta
     2821 agcagggttg cagcggagat cgccagccct ttcagcccac cttgcgggca agcaaagtag
     2881 gccgtacagc ccaggaagcc cgcccagctt agtaagccca gggaaacagc cacccatccc
     2941 cagataccgg agagaatgcc tgttgtgatt gcaatagaga gaagtatgtt catgccgcgc
     3001 atgatagcag aaaacgcggc catgaatgag atcgagcaca cattttatgc aacaacaaat
     3061 tatctgttgc agttatgagt gacgatgatc acatttactc ttcagaatct tcttccagct
     3121 cgtcccacat tgcactaatg gcatcacggg ttagcggtgc catggtgcgc cagaaaggcg
     3181 tggttgcatg ggcctccact ttgccgagga acgcaccaca ccagggtaac agatactcac
     3241 tgaacagtgt ttccagtgct tcgctctcat cttccgttga ctgatcttcc agccaggaag
     3301 ccgcaagcag caatgtgccg atgtgatcgg ctggcgtatc cgccagtagc atccctcgtt
     3361 cggaaagaaa agcgcgcact tccgcttccg ttgcgtcctc aacccatgcg ctacgatatg
     3421 gcggcacggc acattcatcg ccgataaaca acgcattgta atcggcagag acttgcgtca
     3481 tatcacagct tttctgtaaa cgcgtcagta actcatcctg ctccagtggc cagttcgcag
     3541 ccagtttccc ctcacgaatc agagtaaaca gcggcaccag taaaggatct tgcggttggc
     3601 ggtaatagag cgaacccagc acacgacaga ggatagaaaa ctcgttcatt aatatatttc
     3661 cattactagt aattaaaggt ctgcaaattc cgcaatcggt gccataccgc gagattcaag
     3721 gaagttcagt aagcggcacg gagaaacatt caaaatgcgc tctagcggaa aatctaccgc
     3781 gtcgaggatt ttaagacact cttcaaattc ccccatggta aacgcggtgt gagaatccga
     3841 gcctaatgcc acccaacctc cagcatcacg taccgctgca gctaccgcac gacagttgtc
     3901 ttcactgccc ttacgtgagt gtaaaaatga ggaattattg atttccagcg ccacctgatg
     3961 tttcgcggct gcttcagcaa cggctttaac atctatttca tatttcggat ttccgggatg
     4021 gcttattata tgcacattgc cgctggcgat agtggagatc atcgcttgtg tattggtcgc
     4081 tttgtcatgt ggcgcaaaaa ccggctcatg aaaaccggca ataatcagat ccagcgagtc
     4141 aaacatttta ccgctgcagt caatttcacc atcaacattt ttaatgttag cttcaatgcc
     4201 gcgcaggatc cctaccccat caaccactcg cggccagata cgcatgttaa tgaagtgcca
     4261 gtgatgcggc gcatcttcca tatccgggcc atgatcggtg atcgcaaaaa gtttaatgcc
     4321 cttttgtttg gcctgggcaa tgtaatcact caatgtgcta tatgcatgtg tgctggcaac
     4381 ggtatgcata tgaaggtcga cgggatacat aactctctcc tgtattcaat tcccgccaag
     4441 gatagcagga atcctgatgc tttattagta gccacgtgcg cggtcgactt gcccgcagac
     4501 cctctcccct ttttcgagct gggcaatagt gcgagaaatg tactccacag cttcagcggg
     4561 acgggtaatc gcggcgacat gtggtgttat cgtcacgcgt ggatgttgcc agagcggact
     4621 ttcaggcggt aagggttcac gattaaaaac atccagcatt gcgcctttaa ctttcccgct
     4681 atccagcgcc gcgagcaggt catcttccac aacatgaaca ccacgcgcca ggttgaggag
     4741 atacgcgcca tcaggtaatt tttcgagtaa ttgttgatta ataatgccga cggtttcagg
     4801 ggtattcggt aacaaattaa tcaatacccg acattggctc agaaatgcag acagttcttc
     4861 ccgtccggca aagctttgca cgccaggcca cgatttacgg gttcgactcc agcaacgcag
     4921 cggaaagcgc taggtttgca gactctgagc aactttacag cccagtacgc ctgcgcccaa
     4981 aatgccgatg gtaaaatctt cccgatgata ttcaggcagc ggttgccaat gcgaactatt
     5041 ttgctggatg cgataatcgt caaaacgtcg aaaccaatgc agcacctgac tgacagcata
     5101 ttcctgcatt tgctcgccca taccggtatc ttccaggcga aaaagtggaa cagaagggtt
     5161 cagcatttca gggtgtgcct gtagcttgct caaaatagaa tcaacaccgg ccccgagtgc
     5221 gaacaccgct ttaagatcgc gccctgccag catttcaaca ggaggatgcc agactaaaac
     5281 ataatcagca gagtcattat ctccgctttt ccatgccctg actcttgcct gaggaatagc
     5341 tttgcgcagt gcctcaatcc accattgggt atcgaacgtt gggtgataaa agatgatatc
     5401 catactgact cccgaaaagc gttgtgcgaa atttattcgc acttatcgtt atgatctaca
     5461 aaggccacca gcataacaaa tccgtggtcg gtggcaaaaa aagcagattt cgcttattaa
     5521 aaccacacat tgattgaatg tttgaataaa cgcgcgattt tttcaaaaag tttgttgacc
     5581 tcaggtcatg atttccctaa attagcgccc gttccagcaa gacaggaacg acaatttggt
     5641 gaggtgtccg agtggctgaa ggagcacgcc tggaaagtgt gtatacggca acgtatcggg
     5701 ggttcgaatc cccccctcac cgccagatta taagtgcatg ttattgttta acatgcgctt
     5761 ttttattttt ccttccagac agtatgccat cgggtatgcc atctgaaacc ggtttatatg
     5821 ccccgcgatc atatcttatc ggacaatttc cgatctttga gcatactggc aaagctatac
     5881 tgccagcata tcaggatagt gttttgcgat aatatcattc agtctgtcaa ccagttctgg
     5941 ttttctgaac gtgatatgcg cggagccctt cttaaagtat ctgatgctga acatctcatc
     6001 ttcatagctc tctttaccct gaacagactg aatgtgatca tccagacgaa gggtgatgtt
     6061 tccccggttg tcggggattg gtttaccgct gaacaggtgc aacattcttt ccagatcggc
     6121 gagtcggtca gcctgttgtc cggtgataag atgaaatcta tggtcacccc cgtttttgca
     6181 acactgattt ttgattaatg gttggcttgc ttaaatctat ccggcgtctc tgtgggcaag
     6241 gatgcccgcg ccccgatgag ttccgcacct gataatccta aaaatcccct cggcttcagt
     6301 gagccatttc atctgttagg tttttaatca ggtcgatttg ccgttcatgt catcaataat
     6361 cagtcttcgc aaaaccagaa aggtaacttg tatcttattg tttaaaataa actaattgag
     6421 ttatatcagc atctatatca ggcctgatat tcttttttct gagtagtgat tgcccaggca
     6481 attcgagcca gtttatttgc cagagcgcag gtaaccacat tcgaatgctt cttgttgagt
     6541 tgctcccgaa cccactcagc aagttttcca tgcttgcgtt caagtcgcat tatgaatgag
     6601 cgggcacact gaaccagtaa ccggcgtaaa tttttatcac ctcgcttact gatacccaac
     6661 agagtcgatt ttcctccagt gctatactgc cggggaacaa gtcctgtgga agctgcaaaa
     6721 tccctgctgc atgagaactg tttcccgtca ccaagttgcg acgatagtaa gctggcagta
     6781 atcggaccaa caccaggaat tgtcataatg cgctgagcgg tatcatcagc gttgattgac
     6841 tgacttaatt ctgattccag ctctgctatt tgctcaacaa ggtaaaagta gtgggtatgc
     6901 aacctcacca aaaggcggct cagatattcg gggatttcgt gttcagccaa cacgagagac
     6961 aaccgtttta ttacagcatc acctatcggc aggctgattc caaattccag caagaaaccg
     7021 tggatctggt tggttgtttt caccttatct ctgatgagcg attctctgac ccggtgtaac
     7081 gccctcatgg cctgctgggt ctcagttctt ggctggacaa aacgcattga tggtcgtgaa
     7141 gccgcttcgc atatggcttc tgcatcaaca aaatcattct tattactttt gacaaacggg
     7201 cgaacgaact gtggagaaat cagcttagtt tcatgcccaa aatcagagat acagcgagcc
     7261 ataaagtgtg cacccgcaca ggcttccatt acaacaactg agggcggaca tgtcgccaga
     7321 aactgcatca gtttagtacg ggtaaatttt ttacgcagaa gagccttacc agacttgtct
     7381 tgacaatgaa tgtggaagga gtgctttccg agatcgatac caataagtgt gatgttctgc
     7441 atgatggtct ctccgttaaa gggatttacc ttaacagcgt actgctgtta aggtgggggt
     7501 gaccatatca ttatccccac cggtcccatc tcaccagatt gttgacgata attttactgc
     7561 caaatttaca ggggcaattg gttttgtaat tccagttcag ccctctgaag acgttgatca
     7621 cccctcgctc aaacacctca tccttattct ggtgcagttg ttcaaatgtg ctcagaatgt
     7681 tcgcttcgct gatttccgga aaattatcgt actccagtga ccggtaccat gtatcacggg
     7741 cctgcgtatc cattaatgac agcatcccgg aacgttgcat caggtcacgc cagatttcgc
     7801 gatcaagatt ccgggtaatg gctttcattg ctgtttcggg tttctccatc agccagcagc
     7861 cgcagcgaaa atcctgtttc atggcccagt ccggggcagt tttacctccg atgctttccg
     7921 tcagcaccga gatttcagcc agttgtcgga taagtgtgtc gatttgcaac agtgtactct
     7981 ttcgtacagc cagaatgtgg cgaatgctgg ttgagcagat aacgtccgta tgcccgttga
     8041 ccacttccgg acgggagatt gtgatatctg acataatcga tattcctttt tgcagataaa
     8101 tgaaaacgcc agccctgtgc aggctggcgt cagaggaatg aagtctggtg agtgagcttc
     8161 accggttctg gtgcagaagc tgcagatgac gcagggtacg gacatcctcc cgggcctgtt
     8221 cgatacaggg caacagtgct gctgaatccg ttttatcctc atcgttgtca aagataattc
     8281 ccgatccgca gtcgatattg tcctgcagcc acgcaatcag aatatccagc gctgtttccg
     8341 tggttaatga tttcatgttg ttcctggtta ccagtcgaaa gtgggtaaac ccggcaggca
     8401 ttcagcgctg gcatccagat gaatgagact gacgccataa cgccggatga gtgtgacgac
     8461 cagacgacgg aacgtttttg acagccccag gcgtttaagc cgcagaaccg ggtatgacca
     8521 cgcatcggta cgtaacagat atccgctacc tgtaaaatga atccattctg attcaccgaa
     8581 gtcactggtc tggtgtgaca gcgagtacaa ccaggcgttg tccgtttccg tgatatgtgc
     8641 ggtactgcag cgtatgccgg taagggtagc aaatggtggc gtgcattctg ctgaggtctg
     8701 gtcagattca tcaacaacac gaagggagta gccgttatcg cagaccatgt taatcagttc
     8761 agcgagctca ataccatcga cgtcaacgac aatgcgccct atgttcagtg cctgtacgtt
     8821 aacgctgtcg gcttccagtg tcagggccag tttcatcatt tcgcctccgg atacttaccc
     8881 agggtaatgt tatttaccgt tctgtaattg tcgcgggtca tcaggccggt cgcccggcga
     8941 gcccggagga tatcaatact gtttattaac tgagagcggg gacaggtgct gaatcccggc
     9001 tggtcggtac gcaccagcgc gtatttttcc acgagaaaat taactgcatc acacagtgaa
     9061 atgcctgcct caatatgctg ctcaatcaca cgttcatcga caaacggtgt gtcattcagt
     9121 gtgaggccat aatgctggtc cagcagtcgg atgagcagtg tctgccagat ggtgacggga
     9181 gacgggcagc gagatacctc ccgtacatgt gtgtcggata atgttttcat cttaagttct
     9241 ctgtaactgg tagtgctaaa ggtgagagct tcggggttat gaggtggttg cgggtgctgg
     9301 tgtcggataa acagccagat aaacgtaacc acaggagccg agggtgtcgg cttcgcaggt
     9361 cagccctttt gcatacagcg tgacggtatg ctgatatcgg ggattcagtt caccgctggt
     9421 gagcatgagt tccagttgtt tcatcagcag cggaaaggcc tggtccagat ggtatgcatc
     9481 tgcgtcgctg aagcggcctc tgataccggc gcggtcggca aggtaataca ggcggttacc
     9541 ctcttgcacc agacgagcgc caaaacaggg cctcactgtg cagggaagcc cccaccaggg
     9601 gcggtcgtga ttgtcatcgg gatgagttat cccggagagt ttgtctgaca cgataaaatc
     9661 ctctctgaaa agtggttaag aatggtttaa cgatgacagg tcagccggcg cgctgaccgg
     9721 tttcggtgaa gatgtatgca ttgatgagaa tggcctcatc cacagcggcg tctgaggtca
     9781 tctggtcgta ctcgtttccc gggtgacgga acatcagttc agggtgacac tcatcagccg
     9841 gacatacggg ccaaaactgt ccttacggcg ttctgcaaac acggcaagaa cgccgggaat
     9901 atcctgcact tcacgaccag tatacgcttc agcactgccg tgccagcgat acttaccggt
     9961 acagaacgga aaaagacggg atgcaggatg ctgttggtga atacgcatgg cttcaccacg
    10021 ggtgatgatt ctcatgatgt gatacctctg aagacagaag ataaaagtga aaacaggtgt
    10081 gatgtggttg tgacgggtta aagcagacca tgttcggcaa aggagaaaac ctggttgcca
    10141 ccgactatca gatggtccgg cacccggata tccaccaggc ccagtgcctg taccagacgt
    10201 tcggtgataa gccggtctgc cttactgggt gtgacttcac cggacgggtg attgtgcgcc
    10261 agcaccacgg cagcggcatt gtggtacagg gcgcgtttaa tcacttcccg gggatgaact
    10321 tccgtacggt tgatggtgcc ggtgaagagg gtttcaccgg caatcagctg attctggttg
    10381 ttcagataca gcacccggaa ctcttcccgc tccagtcccg ccatgttcag aatcagccat
    10441 tcccgtgccg cacgggtgga ggtgaaggcc acgccgggtt catgaagatg gcggtccagg
    10501 gtttgcaggg cccgcagaat gagactgcgc tcgccgggcg tcatgtttcc gggcagaaag
    10561 gaaagctgct gcattgtccc tgcctccatt cagtcgatga tgcgcataat ggcgctgcat
    10621 tccggatgct gcagggcgta atcccgcagc cggtaataat ggaccgtcat ggcataacac
    10681 tccgtacgac aggcatgatg actgtacgtc atcagacagg cagcaatgcc ggcggcttcc
    10741 gggctcattt cagcgcggtt accgttcatg gcattgaaca gtacccatgt ttcgtcatca
    10801 tcgtcatccg gttcgggtgc cataaatgcc ccgccgttgt tcagggtgta cagattccag
    10861 ataccaccgc agtagtcttc acacagacgg tccatccagc cgaagacacg gggttccagg
    10921 gtgacccact gtggaatgag gccaaaatgc tgcggccaga agctgatgcg ctgttcatca
    10981 gggacgggag tggcaaccag ttgttgcagg ccagtctccg gtgcacaggc cgaagcggtg
    11041 gtgttctgag acaatgtttt catgtatgaa atccttgtaa tgtgaataaa tgagagggag
    11101 taaaggtcgg gagctggcaa catcacgttc atcactgacc gtgatgaccc ggtaatatca
    11161 gggatgaatg atattcggct gaaattccgg acagccgtac gcgtaaccgt tccggtcaca
    11221 gacggatggc cagatgccat tgcctgtata ctcagtctgc acagactgat ttctccggga
    11281 cggtaaagca gacgggtaca ccaggcgcac aggtcatcat cagaaatgga acgacacccc
    11341 ggcagcgcct ccggggcgtc gggtgattct ggtgcctgaa tcacgccttc agggacatgc
    11401 tgcctcagcg caggctctcc agcagcgttt ctgccattac ccacaatgcg cggttgagct
    11461 taatatcggt gtcgatgttg tgaatggcac gggtgtggat acgttttcct tttgcactgc
    11521 ggccggaaat cccgcctttc agcatattct cctggatggt ctgatatgcg ctccacaggt
    11581 ctttaccgta atcctccctg cgtcgtggtg tcagaatgtc agcggtggtg actggctgat
    11641 gttcgtcacc ataacggtaa gtcagtgccg cctgtgccag cgcctggcgt gccggtggcg
    11701 gcaggaccag cgactgcatg gcatcacgct tctcctctat ccggtcaaac acgcccacca
    11761 cctcgtaagc cccttcaata actttctcca ccacatttcc ccggtgtgga acacgcaatt
    11821 ctcccagaga ctgaccgcag accagtccat tggtacaaat ggcacgaaaa tatccgggta
    11881 acatctggta gctggatgaa ccgtcatgag agttgagcag aataatttca gggacatgct
    11941 gaccggttat ctgtccggcc cgccgcagac gcagcatgtg ttttgtgtat tcccggcgac
    12001 ccgggtcacg cacacgtgtc tggcaggcga agaatggctg aaagccttcc cgctgcaggc
    12061 tttccagtac ggtgatggtg ggagtgtacg catagcgttt actgcgggaa gtatgccggt
    12121 cttcaccgaa aatactggga acatagtgca tcagttcttc gtgtgtcagc ggacggtcac
    12181 ggcgtatctg gtttgcagca taaccaaaac gactggctag tcgcataatt tgctccttat
    12241 cggtggttaa gatttactgg tgtaataaat gaaaaagcca cgtctcccgg agaagacgcg
    12301 gcctgatgga cgaaattaat gaggtttatt gtctgagaag accgtcactg gcgaaccttc
    12361 cggcgtcagt ctctgcgttg cggataaccc gacggaaatg agttccctgg tcatcgtcga
    12421 taaacacatt gcggtaagct gttgtgatga caacagcctg ctcgcgtgag aaagggactt
    12481 cgggcaataa acgtgacata caaccagacc tgtatttggt tccctgaaaa tataagtccc
    12541 tgtcatcatg gcagagccgt atttactttt gatatcaata aattaataac gcgaagatga
    12601 accgcataac ttacgccttt aatttgtttt gtttacgtag tccctgacca ggtacgcgaa
    12661 ttcgtgaacg ccagtttcac caaaccaaat caggtaacca gggtcgatgt tgtaatagta
    12721 accaacgact ttatgcgttt ttgggttata gagtgttacg atatagtccc agccgcaggc
    12781 gtgggcttta caaccgccac cttctaccca ggtcgttccg ttaatgttta caggatacgc
    12841 accaccatca ggaccatcca tatagccgtc ttccccaaac attccgttta acccaacagg
    12901 gtttaatgct ttgtcgatag cagctctgta ggtagcgtca ccttcgtata actcgtacgt
    12961 tgttggacat gcaccaggct ggtcagcccg ggcttttgag tcgcatttaa gtgtctgatt
    13021 tacagtggcg gcgtttgtgt caggactt
//
LOCUS       DAZPCS010000102        12908 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZPCS010000102 DAZPCS010000000
VERSION     DAZPCS010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12908
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.102"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(128..1579)
                     /gene="uxaB"
                     /locus_tag="ACHUQ7_002805"
     CDS             complement(128..1579)
                     /gene="uxaB"
                     /locus_tag="ACHUQ7_002805"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFR1136128.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(1806..3224)
                     /locus_tag="ACHUQ7_002806"
     CDS             complement(1806..3224)
                     /locus_tag="ACHUQ7_002806"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFR1136129.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIAFIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(3378..3722)
                     /locus_tag="ACHUQ7_002807"
     CDS             complement(3378..3722)
                     /locus_tag="ACHUQ7_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFR1136130.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVV"
     gene            complement(3722..4648)
                     /gene="glsB"
                     /locus_tag="ACHUQ7_002808"
     CDS             complement(3722..4648)
                     /gene="glsB"
                     /locus_tag="ACHUQ7_002808"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFR1136131.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINTGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(4712..5653)
                     /locus_tag="ACHUQ7_002809"
                     /pseudo
     CDS             complement(4712..5653)
                     /locus_tag="ACHUQ7_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            5727..6842
                     /gene="yneK"
                     /locus_tag="ACHUQ7_002810"
     CDS             5727..6842
                     /gene="yneK"
                     /locus_tag="ACHUQ7_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFR1136132.1"
                     /translation="MVTPVSICNYISLPDDFPARNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNTNSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFMNDFIEQF
                     PMINLGAPIKRAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVQPEVCICFLSLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATAKTPDLPERH"
     gene            6992..8182
                     /gene="ydeA"
                     /locus_tag="ACHUQ7_002811"
     CDS             6992..8182
                     /gene="ydeA"
                     /locus_tag="ACHUQ7_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFR1136133.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(8207..8872)
                     /gene="marC"
                     /locus_tag="ACHUQ7_002812"
     CDS             complement(8207..8872)
                     /gene="marC"
                     /locus_tag="ACHUQ7_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFR1136134.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            9084..9518
                     /gene="marR"
                     /locus_tag="ACHUQ7_002813"
     CDS             9084..9518
                     /gene="marR"
                     /locus_tag="ACHUQ7_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFR1136135.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            9538..9921
                     /gene="marA"
                     /locus_tag="ACHUQ7_002814"
     CDS             9538..9921
                     /gene="marA"
                     /locus_tag="ACHUQ7_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFR1136136.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            9953..10171
                     /gene="marB"
                     /locus_tag="ACHUQ7_002815"
     CDS             9953..10171
                     /gene="marB"
                     /locus_tag="ACHUQ7_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFR1136137.1"
                     /translation="MKPLSSAIAAALILFSAQSVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(10202..11101)
                     /gene="eamA"
                     /locus_tag="ACHUQ7_002816"
     CDS             complement(10202..11101)
                     /gene="eamA"
                     /locus_tag="ACHUQ7_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HFR1136138.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVVSLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            11368..12483
                     /gene="ydeE"
                     /locus_tag="ACHUQ7_002817"
                     /pseudo
     CDS             11368..12483
                     /gene="ydeE"
                     /locus_tag="ACHUQ7_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
     gene            12610..12705
                     /gene="mgtS"
                     /locus_tag="ACHUQ7_002818"
     CDS             12610..12705
                     /gene="mgtS"
                     /locus_tag="ACHUQ7_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HFR1136139.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            12705..12808
                     /gene="mgtT"
                     /locus_tag="ACHUQ7_002819"
                     /pseudo
     CDS             12705..12808
                     /gene="mgtT"
                     /locus_tag="ACHUQ7_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
BASE COUNT         3401 a         3299 c         2950 g         3258 t
ORIGIN      
        1 gtcaagctta caacattaac aggcgctgca tgtgacacct gttaatgata aaggaatata
       61 ggatgttgta actaacatag tgaaataaga aacccggtcg aaaccgggtt cagaagtaac
      121 ggtgttatta gcacaacgga cgtacagctt cgcgcatccc tttttcaaga atcgcatcca
      181 ggtcattagc aacctgctcg accagaccag gcacttgcgt caggtcctgc tcccagtggt
      241 ctttttctgc caacacaatc gctaccagtt cctgggtacc gataacacgg tcacgatgct
      301 ggctccagag ttgctgataa cgttccagcc agtgtgcatc gtcctgtacc ggatatgttt
      361 ctccgttacg ctcaccacga tagaacgcaa tcaatgccgc taatgcgaaa gtaaggcgcg
      421 ccggaagtgt gccatttgcc ttctgccctg ccagcagctg cggcaggatg cgggtgcgga
      481 acttggtcat accgttgagc gcgatagaca gcagctgatg cttaatgtac gggttacgga
      541 aacgcccggt gactgcactg gcgaaagatt ccagttcatc acgaggcaaa tccagtaccg
      601 gaataatttc ttcgtagatg gctttttcaa cgaatgcgca aatttcagca tcgttcatcg
      661 cttcacctac ggtatccagc cctgcctgga acgccaccgg caccagcgcg gtgtgcgcac
      721 cgttgaggat tgccactttg cgttctttat acggtttaat atcgtcaaca atcagcacgt
      781 tgagcggata tttgtccagg cgcagctcag tcgctaagga tttcggcccc tgaatcacaa
      841 acaggtaaaa gtgttcagcg gtgtcgagga aaccatcgtg ataacccagt tcttcttcca
      901 gtttagccac ttcatcgcgc ggataaccgg taacgatacg gtccaccagc gtagaacaga
      961 agctgttagc ctgatccagc cattgaataa atgcttctgg cagtgcccac tcttgcgcat
     1021 agcgcagcac cagttcacgc aacgcgtcgc cattgtagtc aatcaactca cacggaatga
     1081 tgatccaacc tttatccagc gcaccgttga aatggctaaa gcgttcgaac agcagacggg
     1141 tcagttttgc cggatagctt accgctggcg cgtcatcgaa tttatcgccc gcatggtagc
     1201 tgatacccgc ttcggtggtg ttagagaaaa caaagcgcat ttccgggttg tgcgccagtt
     1261 tcaggaattc atcgtattca ctgtagacgc tgatttcacg attaaccgag cgaatcagac
     1321 gcgcgtcgct gaccgcttcc cccttctcat tcaggccacg gataatggtg gtgtacagac
     1381 catcctgcgt gctcagtgac ggcgggaatg aagtttcaat cggacgaaca acgaccacgc
     1441 cagaattcag atcggtgtgc tcattcagga gatcgatttg ccagtcaaca aaggcgcgca
     1501 ggaagttacc ttcaccaaac tgaatgatac gttctggata ctgtgcaccg ggaaaatcgc
     1561 gacgatttag tgttttcaca atgggttccc ttctgattag tcatacaacc cgtttgaatt
     1621 ggtgcgacag gttagcaaac tttaatacgc cgaacccctg ttttgatcaa ctcctgatga
     1681 ttaatgagca gttttatgag aaaagtgtgg cgcggatcat ggtttaatcg aggaaatacg
     1741 ccttttcctg gatcataaag tggtagaaca cattgcattc aaatcgcgcg gaatgaataa
     1801 agatgtcaga caacttcctc accgtaacgc atagtgctgg tacggttgcg cccatctttc
     1861 ttcgagcgat acagacacgt atcggcttcg accatcaatt tattaaaatc atcggtcagc
     1921 gttcggtacg atgcgcgacc actaccgacg ccaatactta ccgtgagata aagcgttttt
     1981 tgttgccagg tgaatggttg cagttcaacg cctttacgga ttttttccgc cattagcaga
     2041 ccatcgacag gattcaccga cggcactgca acagcaaatt cttcgccgcc catgcgcgcc
     2101 accagcccct tatcaccgac aatcttctga atatgccggg caaacacgct taacacttta
     2161 tcgccacatt catgtccata gttatcgtta atgcttttga aatagtcgat atcaagcagc
     2221 atgaccgtca gatgttgtgt ctgtttcagc gatggacttt tcagcgcctc ataaagaccg
     2281 gaccgtgagt aaacctgagt cagaaaatca aagtcggctc gcagcgcaac ttgcttcatt
     2341 agcaaattga tcgctgccac gctaaaagaa accataattg ggcatatcgc catcgtggca
     2401 ataccgagac gtgcggagaa catttccgga atggagaacg gcgaaccgac cgagatatca
     2461 atcaccgaat ttgccaccag tacgatttcc accgcaccgg tgacaaaggt caacagacat
     2521 gttacctgcg gcgtatagcg cactgcacac cagattaatg caggcagcgg gaacgccaga
     2581 ctccccgctc cgccaatgac taccgaggca ataaccgaca caataaacgc gatggcgggc
     2641 atcatctgtt ctgctttaaa gcgcggcagc actccaggaa tagccaacgt cagcatacaa
     2701 ggcacgatca acacgcccgt tgagaattgc tcactgaacc agtccgcaag caaaggccag
     2761 aaatccagac tatcaatact gaccgaacca atcgccccga caatagcgca taataatgcg
     2821 gcaatcagac agtaattaaa tagccgtaaa gcacttactg gctcatactt atttttcccc
     2881 agacgcttat cccgcacgac cagtaaggcg acggtaacaa taaacatcat attggagaaa
     2941 ttgataacca gtgaaaccag tccccattcg gtggttatgg catcataaac cagcatcgcc
     3001 acataactga tcgcatagta atgcagtcga ttaagccaga catagcgggc aaacaccccc
     3061 gccattacgc cattcagcgg ccagaaaaga gaaagttcgt gaaccagccg taactctgca
     3121 ccaataaaat agaacagcgt ggttagcgca aaaatagcga tgctattacg tagcagatga
     3181 ccttcctgga acaaacggaa agttgaaatg gggtgtacat gcattaatta tctgtccata
     3241 caattgcggg tggcaattat ctcaacgtga taagtaaatt ctcataaagg atagcatgtt
     3301 cgctaacagc ggcggcaaat aaaggtaccc tgggaatgct attttatcgt tttccgcaat
     3361 tctcatggct gattcatcta tacaaccagc cagtgataga ttaccgccac gatataacgt
     3421 tgttgttctt cgcttaacga aacaccctgc ggaatgttat gccacttagc cagacaaccg
     3481 cgacagcagg tcgcggtggc gtgctgagcg ataaacaccg gatgcccgcg catgggggtt
     3541 tgcttaccgt cattagccgg taatgccgga gcaagacgtt tagcgacaaa atccgccgca
     3601 tgttgttcga tgactggcgc gcctttctcc aggcaatact gacgctcttt catgcccaga
     3661 cgaaagcgag agcgaaattt tgaacgggat aaacgcgcga agagtggatc gagagactgc
     3721 attaataaac cgaacgccct aactgtttcg tcaattgttc aaggacggcg atacccgcaa
     3781 gcgagttacc tgcatcatcc agttccggac tccagactgc gatggccatt tcatgcggaa
     3841 caatcgccac aataccgcca ccaacgccag atttcgccgg tagtcccacc cgccaggcaa
     3901 actcccccgc gttctggtac ataccactgg tcgccatcag cgcgttaatt tgccgcgcct
     3961 gcattggcgt caccacaggt tcatcaatat gaatagcttt cccctgatta gccagaaaga
     4021 caaacgtccg ggccagctct acacagctca ttttcagagc gcagtaatga aagtagtttt
     4081 gcagaacggt tgtcacgtca tgatggaaat tgccaaacga cttcatcagc caggcgatag
     4141 ccgcatttcg cgcggaatgt tcaaattcgg aacgcgctac caccgtatcg tatgaaatat
     4201 cagacacacc gcttaagccg cgcacgactt ccagcatacg ttgccgtggt gcgcttagtc
     4261 gcccttgcaa catatcgcag accaccagcg caccggtatt aatgaacgga ttacgcggaa
     4321 taccctgttc catttccagt tgcactaaag aattgaacgg tgatccagac ggatctttgc
     4381 cgacgcgttg ccagatttcc tcttcggagt aatgacgcat ggcgacaacg agactcagca
     4441 ctttggaaat agactgaatg gaaaaacgtt cttgcgcgtc tccggcctga aaaagctgtc
     4501 cgtcaacggt acagatagca atccccaatc gggaaccgtc tactgtagcc agcgccggaa
     4561 tataatccgc gactttaccc tgaccaatga gcggccgcac ttgccgcaag atgttttcta
     4621 aaattgcatt atccatggcg actgccactt tctactcctg gaccgcaggt ctgaaaagac
     4681 ctgcgagtat atcagagctg aatatgtcgc gtcagatccg gtctttccac actgtctgga
     4741 tattacagaa ttcgtgtaag ccgaaatggg aaagctcacg accaaagcca ctctttttca
     4801 cgccaccaaa ggccactcgc gcgtcgctgg cacaataacc attgataaac accccaccgc
     4861 attccagacg tgccgccatc tgtctggcct gtgtttcgtc ggtggtaaaa atggtcgctg
     4921 aaaggccgaa ctcactatca ttagccagtt ccagcgcatg ttctgcatct ttcgcaatgg
     4981 tgattgcagc aacggggcca aacatttctt cccgaaacgc ggtcatttct ggggtaacat
     5041 tcgccagaac cgttggcgga tagtagttac ctgctccagc catcttttcc ccgcccagta
     5101 acaaacgcgc accctgcgcc agggttttct ccacctgatg atgcagctca tcacgtaaat
     5161 caaaacgagc cattggtccg agagcgttct cttcatcacg gggatcgccc attttcaagg
     5221 ctgccgcagc tgacacaaaa cgttcggtaa atgccgaagc aattccctct tcgataataa
     5281 agcgttttgc cgctgcacaa acctgtccgg tattctgata acgtccggct accgccgctt
     5341 taaccgccag ttccagatcg gcatcgttaa gcacaataaa tggatccgaa ccgcccagtt
     5401 ccagtacgca ttttttcagt gccgctccag cacgaacact tccggtcacc gtgacagcag
     5461 caatgcgcga gtctttaatc atctgactga caccgtcgtt gtcggcattc agccagccat
     5521 atacgccttg cgggattccc gcatctttaa acacttgggc aatgagctga gcacagccca
     5581 tcacattcgg cgcatgttta agtaagtagc cgttacctgc aagaatgatg ggaacagcgc
     5641 cacgcatcac ctgcctttga tagttcttgc agctcaaatg ccctatatag tgatgttccg
     5701 atgacttatg actatatggg gcaaatatgg ttacgccagt aagcatctgc aattacatat
     5761 ctcttcctga tgattttccc gctcgtaata ttgcacctca agtaaaagaa gttttaaagg
     5821 attttattga tgcactcagt accataatat gtgatgaaga gtggcgcacg tctttaaaca
     5881 ccaactcagc cacaaaaaag atatttaaca accttgataa cttatcatat attcagagga
     5941 cttcttttcg gggtaacgac acactataca atgaaaaggt tcagtttaaa ctcacttatc
     6001 ctgtcaagaa tggaagacac aaagaaaata ttgaatttca ggtagtaata aatttaagcc
     6061 ctatttattt agataatttt cgccatgatg gagaaattaa tattttttgc gctcccaacc
     6121 caaagcctgt cactatgggg cgcgtatttc agaccggcgt cgagcgtgtg ctctttctgt
     6181 ttatgaatga ttttattgaa caatttccaa tgatcaacct tggcgccccc atcaaaagag
     6241 cgcatacacc acatattgaa cccctgcctc cggatcacca taccgctgca gattacttac
     6301 gccagtttga tttgcttgtc ctgaatttta tctctcgcgg taattttgtc atactccccc
     6361 gattatggaa taactctgag gttcacagat ggttcgtcaa taaagatcct aacttgatca
     6421 ccgccattct cgacataacg gacagcgaat taaaagagga tttattgcaa agcctgatgg
     6481 attcattagg ttctaacaaa catgtacaac ccgaagtctg catctgcttc ttatcccttt
     6541 tagcagaaca agaatctcct cattttcagg acttgttttt atttttcgcc aatatgttat
     6601 tgcactatca ccaatttatg aatcccaatg aaagtgatct gaatgacgtg ttgatgccag
     6661 catcattaag tgatgataaa attatcaaac atatggcgcg caggaccctc aaactgtttg
     6721 taaaaaatga aacgccgcca aaagttactc acgaagacct ggtgaaaaac aggcctcgct
     6781 cccctgtcag accacctata cccgcaaccg ccaaaacgcc agacctccct gaacgtcatt
     6841 aaaccgtgat gttaccgact ctctgactcg gaacagaatc agcgtcagag aaacggaaaa
     6901 cgcgatccag atcacaaatg cattgtattc acatcattaa ccgttttaag atcatttcat
     6961 cactttttcg caactcaccc gataatctgt tatgacaaca aacactgttt cccgcaaagt
     7021 ggcgtggcta cgggtcgtta cgctggcagt cgccgccttc atcttcaaca ccaccgaatt
     7081 tgtccctgtt ggcctgctct ctgacattgc gcaaagtttt cacatgcaaa ccgctcaggt
     7141 cggcatcatg ttgaccattt acgcatgggt agtagcgcta atgtcattgc cttttatgtt
     7201 aatgaccagt caggttgaac ggcgcaaatt actgatctgc ctgtttgtgg tgtttattgc
     7261 cagccacgta ctgtcgtttt tatcgtggag ctttaccgtt ctggtgatca gtcgcattgg
     7321 tgtggctttt gcacatgcga ttttctggtc gattacggcg tctctggcga tccgtatggc
     7381 tccggccggg aagcgggcac aggccttgag tttaattgcc accggtacag cactggcgat
     7441 ggtcttaggt ttaccgctcg ggcgcattgt ggggcagtat ttcggctggc gaatgacctt
     7501 cttcgcgatt ggtattgggg cgcttgttac ccttttgtgc ctgattaagt tacttccctt
     7561 actgcctagt gagcattccg gttcattgaa aagcctcccg ctattgttcc gccgcccggc
     7621 attgatgagc atttatttgt taactgtggt ggttgtcacc gcccattaca cggcatacag
     7681 ctatatcgag ccttttgtgc aaaacattgc gggattcagc gccaactttg ccacggcatt
     7741 actgttatta ctcggtggtg cgggcattat tggcagcgtg attttcggta aactgggtaa
     7801 tcaatatgcg tctgcgttgg taagtacggc gattgcgctg ttgctggtgt gtctggcact
     7861 gctgctacct gcggcgaaca gtgaaataca cctcggggtg ctgagtattt tctgggggat
     7921 cgcgatgatg atcatcgggc ttggtatgca ggttaaagtg ctggcgctgg caccagatgc
     7981 taccgacgtc gcgatggcgc tattctccgg catatttaat attggaatcg gggcgggtgc
     8041 gttggtaggt aatcaggtga gtttgcactg gtcaatgtcg atgattggtt atgtgggcgc
     8101 ggtgcctgct tttgctgcgt taatatggtc aatcattata tttcgccgct ggccagtgac
     8161 actcgaagaa cagacgcaat agttgaaagg cccattcggg ccttttttaa tggtacgttt
     8221 taatgatttc caggatgccg ttaataataa actgcacacc catacatacc agcaggaacc
     8281 ccatcaggcg ggagatcgct tcaatgccac ccttgcccac cagccgcata attgcgccgg
     8341 agctgcgtag gcttccccac aaaataaccg ccaccaggaa aaagatcagc ggcggcgcaa
     8401 ccatcagtac ccaatcagcg aaggttgaac tctgacgcac tgtggacgcc gagctaataa
     8461 tcatcgctat ggttcccgga ccggcggtac ttggcattgc cagtggtaca aaggcaatat
     8521 tggcactggg ttcatcttcc agctcttccg acttgctttt cgcctccggt gaatcaatcg
     8581 ctttctgttg cggaaaaagc atccgaaaac cgataaacgc gacgattaag ccgcctgcaa
     8641 ttcgcagacc gggaatcgaa atgccaaatg tatccatcac cagttgccca gcgtaatacg
     8701 ccaccatcat gatggcaaat acgtacaccg aggccatcaa cgactgacga ttacgttcgg
     8761 cactgttcat gttgcctgcc aggccaagaa atagcgcgac agttgttaat gggttagcta
     8821 acggcagcaa caccaccagc cccaggccaa ttgctttaaa caaatctaac attggtggtt
     8881 gttatcctgt gtatctgggt tatcagcgaa aagtataagg ggtaaacaag gataaagtgt
     8941 cactctttag ctaaccttgc atcgcattga ccaaaacttg aaccgattta gcaaaacgtg
     9001 gcatcggtca attcattcat ttgacttata cttgcctggg caatattacc ccctacaact
     9061 aattacttgc caaggcaact aatgtgaaaa gtaccagcga tctgttcaat gaaattattc
     9121 cattgggtcg cttaatccat atggttaatc agaagaaaga tcgtctgctt aacgagtatc
     9181 tgtctccgct ggatattacc gcggcacagt ttaaggtgct ctgctctatc cgctgcgcgg
     9241 cgtgtattac tccggttgaa ctgaaaaaag tgttgtcggt cgacctggga gcactgaccc
     9301 gtatgctgga tcgcctggtc tgtaaaggct gggttgaaag gttgccgaac ccgaatgata
     9361 agcgcggcgt actggtaaaa cttaccacca gcggcgcggc aatatgcgaa caatgccatc
     9421 aattagttgg ccaggacctg catcaagaat taacaaaaaa cctgacggcg gacgaagttg
     9481 caacacttga gcatttgctt aagaaagtcc tgccgtaaac aaaaaagagg tatgacgatg
     9541 tccagacgca atactgacgc tattaccatt catagcattt tggactggat cgaggacaac
     9601 ctggaatcgc cactgtcact ggagaaagtg tcagagcgtt cgggttactc caaatggcac
     9661 ctgcaacgga tgtttaaaaa agaaaccggt cattcattag gccaatacat ccgcagccgt
     9721 aagatgacgg aaatcgcgca aaagctgaag gaaagtaacg agccgatact ctatctggca
     9781 gaacgatatg gcttcgagtc gcaacaaact ctgacccgaa ccttcaaaaa ttactttgat
     9841 gttccgccgc ataaataccg gatgaccaat atgcagggcg aatcgcgctt tttacatcca
     9901 ttaaatcatt acaacagcta gttgataacg tgacaacgtc actgaggcaa tcatgaaacc
     9961 actttcatcc gcaatagcag ctgcgcttat tctcttttcc gcgcagagcg ttgcggaaca
    10021 aaccacgcag ccagttgtta cttcttgtgc caatgtcgtg gttgttcccc catcgcagga
    10081 acaaccaccg tttgatttaa atcacatggg tactggcagt gataagtcgg atgcgctcgg
    10141 cgtgccctat tataatcaac acgctatgta gtttgttctg gccccgacat ctcggggctt
    10201 attaacttcc cacctttacc gctttacgcc accgcaagcc aaatacattg atatacagcc
    10261 cggtcataat gagcaccgca cctaaaaatt gcagacccgt taagcgttca tccaacaata
    10321 gtgccgcact tgccagtccg actacgggca ccagtaacga taacggtgca acccgccagg
    10381 tttcatagcg tcccagtaac gtcccccaga tcccataacc aacaattgtc gccacaaacg
    10441 ccagatacat cagcgacaag atggtggtca tatcaatagt aaccagactg tgaatcatgg
    10501 atgcggaacc atcgagaatc agcgagacaa caaagaaggg aatgattggg attaaagcgc
    10561 tccagattac cagcgacatc accgccggac gcgttgagtg cgacatgatc tttttattga
    10621 agatgttgcc acacgcccaa ctaaatgctg cagccagggt caacataaag ccgagcatcg
    10681 ccacatgctg accgttcaga ctatcttcga ttaacaccag tacgccaaaa atcgctaagg
    10741 cgatccccgc caattgtttg ccatgcagtc gctccccgaa agtaaacgcg ccaagcacga
    10801 tagtaaaaaa cgcctgtgcc tgtaacacca gcgaagccag tccagcaggc ataccaaagt
    10861 taatggcgca gaacagaaag gcaaactgcg caaaactgat ggttaatcca taccccaaga
    10921 gcaaattcag tggtactttc ggtcgtgcaa caaaaaagat ggccggaaaa gcgaccagca
    10981 taaagcgcaa accggccagc atcagcggtg gcatgttatg aagccccact ttgatgacca
    11041 caaaatttag cccccatacg accactacca gtagcgccaa caccccatct tttcgcgaca
    11101 ttctaccgcc tctgaatttc atcttttgta agcaatcaac ttagctgaat ttacttttct
    11161 ttaacagttg attcgttagt cgccggttac gacggcatta atgcgcaaat aagtcgctat
    11221 acttcggatt tttgccatgc gtccgatggt taataacaac gacgaggcaa gaagggcgct
    11281 ggtagagcgt cgtagggata agttcatttg tctgtccggc agtgcgtttc gttatgtttt
    11341 attttagaga tgtaaagaaa tagcatggct acgctgccat ttatgaccat ttacttgagt
    11401 cgccagtaca gcctgagtgt cgatctaatc ggttatgcga tgacaattgc gctcactatt
    11461 ggcgtcgttt ttagcctcgg ttttggtatc ctggcggata agttcgacaa gaaacgctat
    11521 atgttactgg caattaccgc cttcgccagc ggttttattg ccattccttt agtgaataac
    11581 gtgacgctgg ttgtgctctt ttttgccctc attaactgcg cctattctgt ttttgctacc
    11641 gtgctgaaag cctggtttgc cgacaatctt tcgtccacca gcaaaacgaa aatcttctca
    11701 atcaactaca ccatgctaaa cattggctgg accatcggtc cgccgctcgg cacgctgttg
    11761 gtgatgcaga gcatcaatct gcccttttgg ctggcagcta tctgttccgc gtttcccatg
    11821 cttttcattc aaatatgggt aaagcgcagc gagaaaatca tcgccacgga aacaggcagt
    11881 gtctggtcgc cgaaagtttt attacaagat aaagcactgt tgtggtttac ctgctctggt
    11941 tttctggctt cttttgtaag cggcgcattt gcttcatgca tttcacaata tgtgatggtg
    12001 attgctgatg gggattttgc cgaaaaggtg gtcgcggttg ttcttccggt gaatgctgcc
    12061 atggtggtta cgttgcaata ttccgtgggc cgccgactta acccagctaa catccgcgcg
    12121 ctgatgacag caggcaccct ctgtttcgtc atcggactgg tcggttttat tttttccggc
    12181 aacaacctgc tattgtgggg tatgtcagct gcggtattta ctgtcggtga aatcatttat
    12241 gcgccgggcg agtatatgtt gattgaccat attgcgccgc caggaatgaa agccagctat
    12301 ttttccgccc agtctttagg ctggcttggt gccgcgatta acccattagt gagtggcgta
    12361 gtgctaacca gcctgccgcc ttcctcgctg tttgtcatct tagcgttggt gatcattgct
    12421 gcgtgggtgc tgatgttaaa agggattcgt gcaagaccgt gggggcagcc cgcgctttgt
    12481 tgatttaagt cgaacacaat aaaaatttaa ttcagccttc gtttaggtta tctctgctaa
    12541 tatctttctc attgagatga aaattaaggt aagcgaggaa acacaccaca ccataaacgg
    12601 aggcaaataa tgctgggtaa tatgaatgtt tttatggccg tactgggaat aattttattt
    12661 tctggttttc tggccgcgta tttcagccac aaatgggatg actaatgaac ggagataatc
    12721 cctcacctaa ccggcccctt gttacagttg tgtacaaggg gcctgatttt tatgacggcg
    12781 aaaaaaaccg ccagtaaacc ggcggtgaat gcttgcatgg atagatttgt gttttgcttt
    12841 tacgctaaca ggcattttcc tgcactgata acggatcgtt gacacagtag catcagtttt
    12901 ctcaatga
//
LOCUS       DAZPCS010000103        12816 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZPCS010000103 DAZPCS010000000
VERSION     DAZPCS010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12816
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.103"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            211..1725
                     /gene="caiT"
                     /locus_tag="ACHUQ7_002820"
     CDS             211..1725
                     /gene="caiT"
                     /locus_tag="ACHUQ7_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFR1136140.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLAGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            1756..2898
                     /gene="caiA"
                     /locus_tag="ACHUQ7_002821"
     CDS             1756..2898
                     /gene="caiA"
                     /locus_tag="ACHUQ7_002821"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFR1136141.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            3027..4244
                     /gene="caiB"
                     /locus_tag="ACHUQ7_002822"
     CDS             3027..4244
                     /gene="caiB"
                     /locus_tag="ACHUQ7_002822"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HFR1136142.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            4318..5871
                     /gene="caiC"
                     /locus_tag="ACHUQ7_002823"
     CDS             4318..5871
                     /gene="caiC"
                     /locus_tag="ACHUQ7_002823"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFR1136143.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            5980..6765
                     /gene="caiD"
                     /locus_tag="ACHUQ7_002824"
     CDS             5980..6765
                     /gene="caiD"
                     /locus_tag="ACHUQ7_002824"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFR1136144.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            6771..7361
                     /gene="caiE"
                     /locus_tag="ACHUQ7_002825"
     CDS             6771..7361
                     /gene="caiE"
                     /locus_tag="ACHUQ7_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFR1136145.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(7447..7842)
                     /gene="caiF"
                     /locus_tag="ACHUQ7_002826"
     CDS             complement(7447..7842)
                     /gene="caiF"
                     /locus_tag="ACHUQ7_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFR1136146.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(8104..11325)
                     /gene="carB"
                     /locus_tag="ACHUQ7_002827"
     CDS             complement(8104..11325)
                     /gene="carB"
                     /locus_tag="ACHUQ7_002827"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFR1136147.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(11343..12491)
                     /gene="carA"
                     /locus_tag="ACHUQ7_002828"
     CDS             complement(11343..12491)
                     /gene="carA"
                     /locus_tag="ACHUQ7_002828"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFR1136148.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
BASE COUNT         3058 a         3316 c         3358 g         3084 t
ORIGIN      
        1 actgattaat aaccaaaatt aacgaaaaac gccctctgtt cactcgaaac aaaaatgtga
       61 taccaatcac agaatacagc ttattgaata cccattatga gttagccatt aacgcgtcca
      121 cgatgttaat aataattata ttaaatgtta acaaaaataa aacaaacggg aacgcaaaat
      181 aaatattcgt tttcacagtg gaattaactc atgaagaatg aaaagagaaa aacgggaata
      241 gaaccgaagg ttttctttcc gccattaata atcgtcggca tactttgttg gcttacagtc
      301 agagatctgg atgcggcgaa tgtcgttatt aatgctgtat tcagttacgt caccaatgta
      361 tggggatggg catttgaatg gtatatggtg gtgatgcttt tcggttggtt ctggctggtg
      421 tttggcccgt atgccaaaaa gcgtttaggt aacgaaccgc cagaatttag caccgccagt
      481 tggatcttta tgatgttcgc ctcctgtacg tctgctgccg tactgttctg gggatcgatt
      541 gagatctact actacatctc caccccgccg tttggcttag aaccgaactc gacaggagcg
      601 aaagagttgg ggctggctta cagcttgttc cactggggac ctctgccgtg ggccacttac
      661 agcttccttt cagtcgcctt cgcttacttc ttctttgtcc gcaaaatgga agtgattcgc
      721 cccagctcga cactggtgcc gctggcaggt gaaaaacacg ccaaagggtt gttcggtacc
      781 atcgtcgaca acttctatct cgtcgccttg atcttcgcga tgggtaccag tctgggcctt
      841 gccacgccgc tggtgaccga gtgtatgcaa tggttgtttg gcattccgca taccctgcaa
      901 ctggacgcta tcatcattac ctgctggatt atcctcaacg ccatttgcgt cgcttgcggt
      961 ctgcaaaaag gggtacgtat cgccagtgac gtgcgtagtt acctgagctt cctgatgctg
     1021 ggttgggtgt tcatcgtcag cggtgccagc ttcatcatga actacttcac cgattcggtg
     1081 gggatgttgc tgatgtatct gccgcgcatg ttgttctata ccgatcccat cgctaaaggc
     1141 ggcttcccgc agggctggac cgtgttctac tgggcatggt gggtgattta cgccatccag
     1201 atgagtatct tcctcgcccg catctcccgt ggtcgtactg tgcgtgaact gtgcttcggc
     1261 atggtgatgg ggctgacagc atccacctgg atcctgtgga ccgtgctcgg tagtaacact
     1321 ctgctgttga tggataaaaa catcatcaac attccaaatc tgatcgaaca gtacggtgtg
     1381 gcgcgcgcca tcatcgaaac ctgggccgct ctgccactca gcaccgccac catgtggggc
     1441 ttcttcatcc tctgctttat tgccaccgtt acgctggtta acgcctgctc ttataccctg
     1501 gcgatgtcta cttgccgcga agtacgcgat ggtgaagaac caccgctgct ggtgcgtatc
     1561 ggttggtcaa ttctggttgg cattatcggt attgttctgc tggcgctcgg cggcctgaaa
     1621 ccgattcaaa ccgccattat cgccggagga tgcccgctgt tcttcgtcaa cattatggtg
     1681 acgctctcct ttattaaaga cgcgaaacag aactggaaag attaattaac ccccaaaata
     1741 tcaagaggtt gaaagatgga ttttaattta aatgatgagc aggaactgtt tgtcgccggt
     1801 atccgcgaac tgatggccag cgaaaactgg gaggcctatt ttgccgagtg cgaccgtgac
     1861 agcgtctacc cggaacgttt tgtcaaagca ctggcggata tgggtatcga cagtctgctg
     1921 atccctgaag agcacggtgg tctggacgcg gggtttgtta ctctcgccgc cgtgtggatg
     1981 gagctgggac gtctgggggc accaacctat gtactgtacc agctgccggg cgggttcaat
     2041 accttcctgc gcgaaggcac acaagagcag atcgacaaga ttatggcttt ccgcggcacc
     2101 ggtaagcaga tgtggaactc agcgattacc gaaccgggcg cgggctccga cgtgggtagc
     2161 ctgaaaacga cttatacccg tagaaatggt aagatttatc ttaatggtag taagtgtttt
     2221 attaccagta gcgcctacac cccgtacatc gtggtgatgg cgcgcgacgg ggcttctccg
     2281 gacaaacctg tctacaccga atggtttgtt gatatgagca aaccgggcat caaagtgacc
     2341 aaacttgaga agctcggtct gcgtatggat agctgctgtg aaatcacctt tgacgatgtg
     2401 gaactggacg agaaagacat gttcggtcgg gaaggtaacg gctttaaccg cgtcaaagaa
     2461 gagttcgacc atgaacgttt cctggtagcc ctgaccaact acggtacggc gatgtgcgcc
     2521 tttgaagatg ctgcgcgcta cgccaaccag cgcgtgcagt ttggcgaggc tattggtcgt
     2581 ttccagttga ttcaggaaaa attcgcccac atggcgatca aattaaactc catgaaaaac
     2641 atgctgtatg aagcagcgtg gaaagcagac aacggcacca tcacctctgg cgatgcagcg
     2701 atgtgcaaat acttctgcgc caatgcggca tttgaagtgg tggatagcgc aatgcaggtg
     2761 ctgggcggtg tcgggattgc gggcaaccac cgcatcagcc gcttctggcg tgacctgcgt
     2821 gtagaccgcg tttccggagg atctgacgaa atgcagatcc tgacgctggg tcgtgcggtg
     2881 ctgaagcaat accgctaagt ccgtgcgctg cctgatgcga cgctgacgcg tcttatcagg
     2941 cctacgggag cacgaatgta agccggataa ggcgtttacg ccgcatccgg cagtcatgcg
     3001 ctacataaat ttttcaggag ctaataatgg atcatctacc catgccgaaa ttcggaccgc
     3061 tggccggatt gcgcgttgtc ttctccggta tcgaaatcgc cggaccgttt gccggacaaa
     3121 tgttcgcaga atggggcgcg gaagttattt ggatcgagaa cgtcgcctgg gccgacacca
     3181 ttcgcgttca accgaactac ccgcagctct cccgccgcaa tttgcacgcg ctgtcgttaa
     3241 atattttcaa agatgaaggc cgcgaagcgt ttctgaaatt aatggaaacc accgatatct
     3301 tcatcgaagc cagtaaaggt ccggcctttg cccgtcgtgg cattaccgat gaagtactgt
     3361 ggcagcacaa cccgaaactg gttatcgctc acctgtccgg ttttggtcag tacggcaccg
     3421 aggagtacac caatcttccg gcctataaca ccatcgccca ggccttcagt ggttacctga
     3481 ttcagaacgg tgatgttgac cagccaatgc ctgccttccc gtataccgcc gattactttt
     3541 ctggcctgac cgccaccacg gcggcgctgg cagcactgca taaagtgcgt gaaaccggta
     3601 aaggcgaaag tatcgacatc gccatgtatg aagtgatgct gcgtatgggc cagtacttca
     3661 tgatggatta cttcaacggc ggcgaaatgt gcccgcgcat gagcaaaggt aaagatccct
     3721 actacgccgg ttgcggtctg tataaatgtg ccgacggcta catcgtgatg gaactggtgg
     3781 gcattaccca aattgaagag tgctttaaag atattggcct cgcacatcta ctcggtacgc
     3841 cggaaatccc ggaaggcact cagcttatcc accgtatcga atgcccttac ggcccactgg
     3901 tagaagaaaa actcgatgcc tggctggcgg cacataccat cgcagaagtt aaagaacgct
     3961 ttgccgaact gaatatcgcc tgtgccaaag tactgaccgt accggaactg gaaagcaatc
     4021 cacagtatgt tgcccgtgaa tcaatcactc agtggcaaac gatggatggt cgcacctgca
     4081 aagggccgaa catcatgccg aaattcaaaa ataaccccgg acaaatctgg cgcggaatgc
     4141 cctcacatgg catggacacg gctgccattt tgaaaaatat cggctacagc gaaaacgaca
     4201 ttcaggagtt ggtcagcaaa ggtctggcca aagttgagga ctaaacgcgt ggcgtcagtc
     4261 ggcttgcctg actagcgccc gcccaataac aaacgaacaa aaatggatag aggtgcaatg
     4321 gatatcattg gcggacaaca tctacgtcaa atgtgggacg atctggcgga cgtttacggt
     4381 cataaaacgg cgctgatttg tgaatccagc ggcggagtcg ttaaccggta tagttatctt
     4441 gagttaaatc aggagattaa ccgcacggca aacctgtttt atacgctggg gattcgcaaa
     4501 ggcaacaagg ttgcactaca tctcgacaac tgcccggaat ttatcttttg ctggttcggg
     4561 ctggcaaaaa ttggcgcgat tatggtgccg attaacgccc gcctgttacg cgaggaaagc
     4621 gagtggatct tgcaaaatag ccaggcgtgc ctgctggtga ccagtgcgca attttatcct
     4681 atgtatcaac agattcagca ggaagatgcc actcaattgc ggcacatttg cctgacagat
     4741 gtggcacttc ccgctgatga tggagtgagt tcatttactc aactgaaaaa tcaacaacct
     4801 gccaccttgt gctatgcacc gccgctatcg actgacgata cggcggaaat tctcttcacc
     4861 tccggcacca cctcccgacc gaaaggtgtg gtgattaccc attacaacct gcgcttcgct
     4921 ggatattact ccgcctggca gtgtgcactg cgtgacgatg acgtctacat gacggtaatg
     4981 cctgcgtttc atatcgattg ccagtgtact gcggcgatgg cggcgttttc tgccggggcc
     5041 acctttgtgc tggtcgagaa atacagcgcc cgcgccttct ggggacaggc gcagaagtac
     5101 cgcgccacca ttaccgaatg tattccgatg atgatccgta cgttgatggt gcagccgcct
     5161 tcagcgaacg atcggcaaca ccgcctgcgg gaagtgatgt tttatctcaa cttgtcggag
     5221 caggaaaaag acgcattttg tgaacgcttc ggcgttcgct tgctgacgtc ttatgggatg
     5281 acggaaacca ttgtgggcat tatcggcgat cgccctagcg ataaacgacg ctggccgtcg
     5341 attggtcggg cggggttttg ctacgaagcg gagatccgcg acgatcacaa tcgcccgctc
     5401 ccggcaggtg agatcggtga aatctgtatt aaaggcgtac cagggaaaac catcttcaaa
     5461 gagtattttc tcaacccgaa agccactgcg aaagtgctgg aagccgacgg ctggctacat
     5521 accggcgata ccggataccg cgacgaagaa ggcttttttt atttcgtcga tcgccgctgc
     5581 aatatgatca aacgcggtgg cgagaatgtc tcctgcgtgg agctggaaaa tatcatcgcc
     5641 acccatccga aaattcagga catcgtggtt gtgggtatta aagattcgat tcgcgatgaa
     5701 gccatcaaag catttgtggt gctgaatgaa ggtgaaacat tgagcgaaga ggaatttttt
     5761 cgcttctgcg aacaaaatat ggcgaaattt aaagtgccct cttatctgga gatcagaaaa
     5821 gatctgccac gtaattgctc ggggaaaata attagaaaga atctgaaata aatgaaacgt
     5881 cagggaacta ccctgccagc taataatcac gccctaaaac aatatgcatt ttttgcaggg
     5941 atgttgtcgt ccctaaaaaa gcaaaaatgg agaaaaggaa tgagtgaatc attacatctg
     6001 acccgcaatg gatcaattct ggaaattacc cttgatcgcc caaaagcgaa tgctattgat
     6061 gcaaaaacca gctttgaaat gggcgaagta tttctaaatt tccgtgacga tccgcaatta
     6121 cgtgtcgcca ttattaccgg tgccggagag aaattctttt ccgcgggctg ggatttaaaa
     6181 gcggcagcag aaggcgaagc accggatgct gactttggcc cgggtggttt tgcgggatta
     6241 accgaaattt tcaatctcga caaaccagtt atcgcagctg tgaacggcta cgcctttggc
     6301 ggcggtttcg aactggcgct ggcggcagat tttattgttt gtgccgataa cgccagcttc
     6361 gccctgccgg aagccaaact gggcatcgtt cctgacagcg gcggtgtgct gcgtctgccg
     6421 aagatcctgc cgcctaccat cgtcaatgaa atggtgatga ccggcagacg aatgggcgca
     6481 gaagaggcgc tgcgttgggg gatagtcaac cgcgtggtta gccaggcgga actgatggat
     6541 aacgcccgcg aactggctca gcagctggtt aacagcgccc cgctggcgat tgcggcgctg
     6601 aaagagatct accgcaccac cagcgaaatg ccggtagaag aagcgtatcg ctatattcgc
     6661 agcggcgtgt tgaaacacta tccatcggtt ctgcattcgg aagatgccat tgaagggccg
     6721 ctggcgtttg ccgagaagcg cgatccggtg tggaaaggac gttaacaacc gtgagctatt
     6781 acgcctttga ggggttaatt ccggtggttc acccgacggc gtttgtccat cccagtgccg
     6841 tcttgattgg cgatgtgatt gtgggagccg gtgtctacat cggcccactc gcctcactgc
     6901 gtggtgacta cgggcggttg atcgtgcaag cgggagccaa tattcaggat ggctgcatta
     6961 tgcatggcta ctgcgacact gacaccatcg ttggggaaaa cggccatatc gggcatggag
     7021 cgatcctgca tggttgtgtg attggtcgcg atgcattggt cgggatgaac agcgtaatta
     7081 tggatggcgc ggtcattggc gaagagagca ttgttgccgc aatgagcttt gtcaaagcag
     7141 gctttagtgg cgagaaacgc cagttgttga tgggtacgcc agcccgcgct gtacgcagtg
     7201 ttagtgacga cgagttacac tggaaacggt tgaataccaa agagtatcag gatcttgtcg
     7261 ggcgctgtca tgcatcgtta catgaaacgc agccactgag gcaaatggag gaaaatcgcc
     7321 cccgtttgca ggggacgacg gatgtgacgc ctaaacggtg atcttgccgg atgcgacctg
     7381 gttgctggat aagatgctac aacatcgtat ctggcaagca ggacgcccgg tatcaacgag
     7441 ataaaattaa cgacgcatac tctttgacaa catcatctgc cacttctgct cgcggttaag
     7501 ttccgtgagc ggaacggcag gaatacgcgg tgtctttctt acgccgacta atttttcccg
     7561 actgttattg cgcaggcgcg cgtaaatttg ctcatcgata tcgaccactt taaccagtcg
     7621 ctgacactgg catccccgcc cttccagctt attagggatc atcttaactt cgcagcttat
     7681 ttctgtgact tccgacagaa tataagtcag ggtattaacc gccttgctgt gttcaatatc
     7741 gaaatgaata gagatctctc ttgctatcac ccaccgattt tcagccatca tccattcggc
     7801 gattaacaag tagagtggtt tttcaacata tccttcacac atacgaactc cagtttcaca
     7861 aattaaaagt ctagctatga ctcgttttga agcaattaaa caaggccata ttaaaattgt
     7921 ggatatatcg atagtatgca acttcaccgt cgataagtgt gagctaaatc ctgcttatgt
     7981 catcaaaaat atcgattccc caaaagattt acttaatggg caaaaaaaaa cggtcctcat
     8041 cagagaaccg tattagttat tcgatcaaat tagcggatga aaaatatccg ccacgacacg
     8101 ctattatttg atctgtgcgt gcatttcctg caccgaaatt actttttcag tcgcatcggc
     8161 attcagcgcc atcgcggtag cgaaaccacc gttcagggtg gtgtcataat gcactttata
     8221 ttgcagcgca ctgcgacgga tcacgcggga gtcttcaatc gcacgacggc ctgaggtggt
     8281 gttgatgatg taggtatatt cgccattctt gatacggtcc tgaatgtgcg gacggccttc
     8341 atgcaccttg tttaccagac gcggattgat acccgcttcg cccagcacaa tcgccgtgcc
     8401 gtgggtcgca tccagctcga agccctgttt cagcagtttt gccgccaggt ccaccacgcg
     8461 ttctttatcg ccttcgcgca cggaaagcag cgcacgaccg tgtttcttca tggtggagtt
     8521 gctgcccagc tgcgctttgg cgaacgcttc agcgaaggtg cggcccacgc ccatgacttc
     8581 cccggtagag cgcatttctg gccctaacag cgggtcaacg cctgggaatt tattgaacgg
     8641 cagcaccact tctttcaccg agtagtacgg cggaataact tctttggtta cgccctgctc
     8701 agccagcgat ttgccagcca tcacacgcgc cgccactttt gccagcggta cgccggtggc
     8761 tttggagacg aacggaacag tacgcgccgc acgcgggttt acttcaatca ggtaaacttc
     8821 gttgtttttc accgcaaact gcacgttcat cagaccgcgc acctgcaatt cgaaggccag
     8881 tttctgcacc tgctggcgca tcacatcctg aatttcctga cttaaggtgt acgctggcag
     8941 cgaacatgcg gagtcaccgg agtgcacacc cgcctgctcg atgtgctcca tgatgccgcc
     9001 aatcagcacc atttcgccgt cgcagatggc atccacgtca acttctactg cgtcatcaag
     9061 gaaatgatcc agcaacactg gcgcatcgtt agacacgcta accgccgtct ggaagtagcg
     9121 acgcaggtca gcttcgtcat agacgatttc catcgcccga ccgccgagaa cgtaagacgg
     9181 acgcaccacc agcgggtagc caatctcttt tgccttctca actgccattt caatagcagt
     9241 aacggtggcg ttcgccggtt gtttcagttt cagacggtca accgcatgct ggaagcgttc
     9301 acggtcttcc gcacggtcga tagcatccgg gctggtgccg ataaccggta cgccagcggc
     9361 ttccagtgca cgcgccagtt tcagcggggt ctgaccgccg tactgaacga taacgccttt
     9421 cggcttctca atacgcacga tttccagcac atcttccaga gttaccggct cgaagtagag
     9481 gcgatcggaa gtgtcgtagt cggtagagac ggtttccggg ttacagttaa ccataatggt
     9541 ttcgtaaccg tcttcacgca gcgccagcga ggcgtgtacg cagcagtagt cgaattcgat
     9601 accctgaccg atacggtttg gaccgccgcc aagcaccatg attttttcac ggtcggtaga
     9661 cggattcgct tcgcactctt cttcataagt ggagtacatg taagcggtgt cggtggcaaa
     9721 ctctgccgca caggtatcca cgcgcttgta gaccgggtgc aggtcatatt ggtcacgcag
     9781 cttacggatt tccgcttcgc gtacgcccgc cagttttgcc aagcgcgcat cggcaaagcc
     9841 tttgcgtttc agctggcgca ggaattcagc gttcaggcca gtgatgccca cttccgccac
     9901 tttctcttcc agacgcacca gttcttcaat ctgtaccagg aaccagcggt caatgttagt
     9961 caggttgaag acgccgtcca cagacaggcc cgcgcggaaa gcatcggcga tgtaccagat
    10021 acgctctgcg cctgcgtctt tcagttcgcg acggattttg gttaacgctt ccgggtcatc
    10081 caggctcact ttcgggtcga agccagttgc gcccacttcc aggccgcgca gcgctttttg
    10141 cagggattcc tgctgcgtgc gaccaatcgc catcacttcg ccaaccgatt tcatctgagt
    10201 ggtcagacgg tcgttagcac cggcgaattt ttcgaagttg aagcgaggaa ttttagtaac
    10261 cacatagtcg atggacggct cgaaggaggc cggagtacgt ccgccagtga tgtcgttcat
    10321 cagttcgtct agggtgtaac ccaccgccag tttcgccgcc actttagcaa tcgggaaacc
    10381 ggtcgctttc gacgccagcg ccgaagaacg ggacacgcgt gggttcattt cgataacaat
    10441 caggcgaccg tttttcgggt tcaccgcaaa ctggacattg gaaccaccgg tttcaacgcc
    10501 gatttcacgc agcaccgcca tcgaggcgtt acgcatgatt tgatattctt tgtcggtcag
    10561 cgtttgggct ggcgcgacag tgatggagtc accggtgtgg atgcccatcg catcaaagtt
    10621 ttcgatagag cagacgatga tgcagttgtc gtttttatca cgcaccactt ccatctcgta
    10681 ctctttccag ccgatcagcg actcatcaat cagcaactct ttggtcggag agagatccag
    10741 accgcgggcg caaatttctt caaactcttc acggttataa gcgataccgc cgccgctacc
    10801 gcccatggta aaggatgggc gaataatgca cgggaagccc acgtcagcgg caacagccag
    10861 cgcttcttcc atcgtgtgtg cgataccgga acgcgcggtt tccagaccaa ttttcttcat
    10921 cgctacgtcg aaacgacggc ggtcttctgc tttatcaatc gcatcggcag tggcaccaat
    10981 catggtgaca ccgaactctt ccaacacgcc ctgacgttcc agctccagcg cgcagttcag
    11041 cgccgtctga ccgcccatgg ttggcagcac cgcgtccggg cgctcttttt caataatctt
    11101 gcgtaccact tcccagtgaa tcggctcgat gtaggtcgca tcggccattt ccgggtcggt
    11161 catgatggtc gccgggttgg agttcaccag aatgacgcgg taaccctctt cgcgcagggc
    11221 tttacatgct tgcgcgccag agtagtcaaa ctcacacgcc tgaccgataa caatcgggcc
    11281 cgcacccaga atcaggatac tttttatatc tgtacgtttt ggcatggctc ttttactcct
    11341 gattacttag cggttttacg gtactgctca attaacttga taaagtggtc gaacaacggc
    11401 gcggcgtcgt gtggaccagg gctggcttca gggtgaccct ggaagctgaa cgccggctta
    11461 tcgatgcgat gaatgccctg taacgtaccg tcgaacaggg atttatgcgt gacacgcagg
    11521 tttgcaggta atgttgcttc atccaccgca aaaccgtggt tctgggcggt gatcatcacc
    11581 acgtttttct ccacatcttt aaccggatgg ttgccgccgt ggtgaccaaa tttcattttg
    11641 acagtcttcg caccgctcgc cagcgccagc agctgatgac cgagacagat gccgaatacc
    11701 ggaatatcgg tttcgaggaa cttctggatg gcggtaatgg cgtaatcgca cggggccggg
    11761 tcgccaggac cgttggagag gaagatgccg tctggattca ttttcagcac atcttccgca
    11821 gaagtttgcg ccggaacgat ggtcaggcga cagcctctat ccaccagcat ccgcaggatg
    11881 ttgcgcttgg caccaaaatc ataagccacg acgtggaacg gcagctcgtc ttcttttttc
    11941 gcttctggca ggccgccggt caacgtccag ctcccttgtg tccagctata ggcttctgcg
    12001 gtggtcactt cttttgccag atccatgccg ttcagacctg ggaacgcgcg ggctttttct
    12061 aacgccagcg ccgcatccgg gttatcgcct gcgataatgc agccattctg tgcgcctttc
    12121 tcgcgcagta aacgcgtcag cttacgggta tcgatatcgg caatcgccac gatgttatgg
    12181 cgcttcaggt aagaagagag gtcttcggta ttacggaagt tgctggcaat cagcggcagg
    12241 tcgcgaatca ccagaccttg tgcatgtacc tgagaagatt cttcatcggc gtcattagtg
    12301 ccgacattgc caatatgggg ataagtaaga gtaacgattt gacgagaata ggaaggatca
    12361 gtgaggattt cttgataacc ggtcattgaa gtattgaaaa cgacttcccc aaccgccgaa
    12421 cctgttgccc ctatggcccg accgtgaaac tgggttccgt cttccagaac caatagcgct
    12481 gacttaatca aaacaccctc cagagaatac tcactcactt tatttgcata ttaattcaca
    12541 gtgatgacgt aaatcaatgc aaatctgctt accgacgaat ttctggcaaa cggcggcatt
    12601 ctggagatat gggcgctaaa agtcaactta atggcaggat tttttatatt attttgtggc
    12661 tcatgcatgt tttacaggca ttaagcgaca aaaacatcat ctaacctgtt ctggaaaacg
    12721 cttgcgcagc aagaagattg catgaaataa aaacgagcag gaaaaagtgg accaaatggt
    12781 caaaatttac attaaaacaa caaaaataga agacga
//
LOCUS       DAZPCS010000104        12733 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZPCS010000104 DAZPCS010000000
VERSION     DAZPCS010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12733
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.104"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..1038
                     /locus_tag="ACHUQ7_002829"
     CDS             <1..1038
                     /locus_tag="ACHUQ7_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFR1136149.1"
                     /translation="FFPNLVDHLLVKHHTADGVEDGISICYFKPYSQGRARWQGDTIH
                     GVWFDEEPPYSIYGEGLTRTNKYGQFSILTFTPLMGMSDVVTKFLKNPSKSQKVVNMT
                     IYDAEHYTDEQKEQIIASYPEHEREARARGIPTMGSGRIFQIPEETIKCQPFECPDHF
                     YVIGGMDFGWDHPQAQVQLWWDKEADTIYVSRVWKAKEKTAVQAWGAVKSWAHKVPTA
                     WPHDGNQHEKGGGEQLKGQYADAGFMMLQEHATWPDGGNAVEPGITELRDMMLDGRFK
                     VFNTCEPFFEEFRLYHRDENGKIVKLNDDVLSAVRYAYMMRRFAKMMRDIKKPKEKKI
                     PAPIRPIARRT"
     gene            1039..3204
                     /locus_tag="ACHUQ7_002830"
     CDS             1039..3204
                     /locus_tag="ACHUQ7_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFR1136150.1"
                     /translation="MADENRLNSILCKFDADWMASDEARTEATNDLYFSRVSQWDDWL
                     SNYTTLQYRGQFDVVRPEVRKLVAEMRRNPVDVLFRPKDGANPDAADVLMGMYRTDMR
                     HNTAKIAVNVGVREQIESGVGAWRLVTQYEDNDPTSNNQVIRRLPIHEACSHVIWDAN
                     SKQMDKSDAKHCTVINALSRNGWKEFAEDYGIDPDTLPSFQNPNDTWLFPWVSNDVVY
                     VAEYYEVEEEKEKVFIYRDPLTGEPVSYYQQDIKDVIDDLANRGFIKVAERKVKRRRV
                     YKSIITCTQILKDREKIAGEHIPIVPVYGEWSFAGDKERYEGVVRLTKDGQRLRNMIM
                     SFNADIVARSPKKKPTFFPEQIEGYEYMYGGNDDYPYYLQNRTDENGNDLPIGPISYM
                     ENPEVPQANAYMLEAATNAVKEVASLGVDAQAANGQVAFDTVNQLNMRADLETYVFQD
                     NLATAMRRDGEIYASMVNDIYDVPRHVTLTLEDGSEKDVQLYAQVVDYQSGNVVTLND
                     IRGRYECYTDVGPSFQSMKEQNRAEIQELLTKVPQGTPEFQMLMLQYFTLLDGKGVEM
                     MREYANKQLVMMGLKKPETPEEMEMVQQAQQQPQQPSAEQIQAQGILLQGQAELLKAE
                     NQQAQIQVEAAKVEAQNQLNAAKIAEIFNNMDLDKQAELREYLRLVGQFQQQRSKDAR
                     ANAELLLKDADQTHSQRMDFANLMRQVQIPSGGVAETPQ"
     gene            3218..4129
                     /locus_tag="ACHUQ7_002831"
     CDS             3218..4129
                     /locus_tag="ACHUQ7_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein"
                     /protein_id="HFR1136151.1"
                     /translation="MDQTTDIQASEELTLPGNHAAASADGLVVDNANDSAGQEEGFEI
                     VRKDDEKPKQDPATNAEFARRRIERKRQRELEQQMEAVKRGELPEHLRVNPELPKQPD
                     PNDYLSEDALAKYDYDQSRALAAFQQANSEWQIKAMDARSQAVAEQGRKTQEFTQQSA
                     QYVEAARKHYEAAEKLNIPDYQEKEDAFMQLVPPAVGADIMRLFPEKSAALMYHLGAN
                     PEKTRQLLAMDGQSALIELTRLSERLTLKPRAKPVSEAPLPDEPIQGHAVAANISAIE
                     KQMEAAANKGDVETYRKLKAQLNKGIR"
     gene            4129..5424
                     /locus_tag="ACHUQ7_002832"
     CDS             4129..5424
                     /locus_tag="ACHUQ7_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22 phage major capsid protein family protein"
                     /protein_id="HFR1136152.1"
                     /translation="MALNEGQLVTYALDEIIGTVQNLTPMASKVTKYPPPAESMQRSS
                     NTVWMPVEQEAPTQTGWDLTGNATGILELSVKCNMGDPDNDFFELRADDLRDERSYRR
                     RIQASAKKLANNIESAIAKQATEMGSLVVHDTRAIGPSTGLPGWDFVSDAERLMFSRE
                     LNRDMGISYFLNPDDYRKAGRNLVDGDIFGRVPEEAYRNGTIQRQIAGFDEILRSPKL
                     PAVTKSTATGVTVSGAQKFKPQAYTLDTDGNKENVDNRVATVTVSSTTGFKRGDKISF
                     IGVKFLSQMAKNVLTDDATFSITRVIDGTHIEITPKPIALDDASLTKEEKAYANVNTS
                     LADTTPVNVLNVATTTANVFWADDSIRLLSQPIPVTHELFAGMKTSSFSIPGIGVNGI
                     FATQGDINTLSGKCRIAVWYSACAVRPEAIGVGLPNQTA"
     gene            5469..5699
                     /locus_tag="ACHUQ7_002833"
     CDS             5469..5699
                     /locus_tag="ACHUQ7_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1136153.1"
                     /translation="MTHMIFRHGDMKKWKGVGYDFEIVKAEELQEYLDAGWFSHPDDL
                     LKDVAEPEPEEKQRKKPGRKPKAAADEPDNEG"
     gene            5677..6177
                     /locus_tag="ACHUQ7_002834"
     CDS             5677..6177
                     /locus_tag="ACHUQ7_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization gp4 family protein"
                     /protein_id="HFR1136154.1"
                     /translation="MNLTTKGDLVLAALRKLGVASNATLTDVEPQSMEDGVNDLEMMM
                     AEWLGGDASPGINVGYIFADADVAPDPGDEHGLSNNAINAVIFNLACRIAPDYALEAS
                     AKLITTARYGKERLVKLSAMDRAKAAKCKSGYPNRMPVGSGNQLAKWNGWNYFHRKEP
                     CDNGSE"
     gene            6177..7595
                     /locus_tag="ACHUQ7_002835"
     CDS             6177..7595
                     /locus_tag="ACHUQ7_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HFR1136155.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPTDSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIDSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSIANGPQWCVLKTGLY
                     DDVYRAIDFVYEGNQITCGDKLESVTGKLQFDISSQYDKQQEHLLFTPLFKADNARVF
                     DLEVESSTGVAQYADRLFLSATTDCINYGREQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGAQIRIE"
     gene            7595..8443
                     /locus_tag="ACHUQ7_002836"
     CDS             7595..8443
                     /locus_tag="ACHUQ7_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail needle knob protein"
                     /protein_id="HFR1136156.1"
                     /translation="MADSNLNVPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILADHEQRISAAEATLVNHEERIRQAESTLQDHE
                     TRIAQNESDISSLDTRVQSLESQVSDHETRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGSLAPFFDTVNNKMVVFNENKTLLFKLSIVGTWPSGTANRSMQL
                     TFSGSVPDTLVSSRNAATTTDNILLATFFSVDKDGFLATNGSTLTIQSNGAAFTATTI
                     KIIAEQ"
     gene            8443..8898
                     /locus_tag="ACHUQ7_002837"
     CDS             8443..8898
                     /locus_tag="ACHUQ7_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HFR1136157.1"
                     /translation="MITFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTLDCHAMYLPEIRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            8901..9593
                     /locus_tag="ACHUQ7_002838"
     CDS             8901..9593
                     /locus_tag="ACHUQ7_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HFR1136158.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSKQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNYNNLANVGLGALQGQANAG
                     QTYANNMSSIAQQSAALAAANANKPSSLQTAISGGTSGAIAGAGLASLLGTSTPWGAG
                     IGAGIGLLGSLF"
     gene            9603..10934
                     /locus_tag="ACHUQ7_002839"
     CDS             9603..10934
                     /locus_tag="ACHUQ7_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HFR1136159.1"
                     /translation="MATWQGTNGGLLAGIGGVNSNAPSVNDIGNTLQLIRQNNDIERS
                     GANNVGLTALQGLSGIAGVFQQEKQAQRQKEFQQAYANAYASGDRGALRQLATQYPDQ
                     IESVRKGMGFIDEEQRNSIGTLAAGARLASSSPEAMQSWLQNNAGELARVGVNPHDVA
                     QMYQQNPRQFGEFVDHLGMNSLGPEKYFDLQDKMQGRQVTMRGQDLDSQTAARNQAIT
                     MRGQDIQANLGQQRINLDAETNRINNENKRLDRMLSAETNDLKRQEIQSRIAANNQQL
                     QQKQQALNDGYKDGINTLTTSMFTLNDIVSSPSLKSITGLRGVIPNVPGSQAADTQAR
                     LDTFKSQAYLTAVQAMRGMGALSDAEGKKLDQAVGSLQNSQSEESFRRNAGVILNTLN
                     QKRNEAVGKYVQQNGIKRVEAPQASIDYLKQHPELSIDFINRYGYLPSLGQ"
     gene            10935..12665
                     /locus_tag="ACHUQ7_002840"
     CDS             10935..12665
                     /locus_tag="ACHUQ7_002840"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFR1136160.1"
                     /translation="MANYRDLLEQAGARYGVPEGLMTALGAKESSYNPAAVSSAGAVG
                     LTQVMPGTWRDMGYTDEQMQNPEYQADAGARYLAKMYQQFGNWRDSLQAYHDGPGNVM
                     KAKRGEYTPGPEGRGYVDDRFAQWAGDPVTDSTVEQRATSAKVHPQQDPNNPFAQLEV
                     QSSEQVSASGVQSDPNNPFAQIEQQAASQQPPQPVSSVAPKPVQQQTVNQANNAPTRE
                     EPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGGPKVLD
                     DVYRPVDRPTDPYAQAGESIGGYLVPGAGVAGNMVIGSLADAANQRGDFAENAAINAG
                     LSIATHGLINGVTRGVRGASNIISGNKTSAQRATTAPTETSPFSGDAAAATNPAVHAA
                     EARVAQGVPMTPATKNPEEVVRTVAAQKRPNLASSLDELDINPQAEVLESAERLNVDS
                     LLPSHFSGNEQYKAVEQAIKSRAGSALQVQENEAIRQLAQGAGEIIDRVSGAKDALGM
                     SDKFIDTVNGRMSALMKRSDQLYRNVEKAMPAGAKIDAPSTRSMLKQVAEDLGGMKNL
                     DPIEKRVEVA"
BASE COUNT         3435 a         3145 c         3355 g         2798 t
ORIGIN      
        1 ttctttccga accttgttga tcaccttctg gttaagcatc acacggctga tggcgttgaa
       61 gatggcattt caatctgcta cttcaagcca tactcgcaag gccgtgctcg ctggcagggt
      121 gacacaatcc acggcgtgtg gtttgacgaa gagccaccat acagcattta tggcgaaggt
      181 cttacccgta ccaacaaata cgggcaattc tcaattctga cgtttacccc gctgatgggg
      241 atgtctgacg ttgttaccaa gttcctgaag aaccccagca agtcgcagaa agtggtcaac
      301 atgaccatct atgacgctga gcactatacc gacgagcaga aagagcaaat catcgcatca
      361 tatcccgagc atgagagaga ggcgcgtgct cgcggtattc ctacgatggg tagtggtcga
      421 atcttccaga taccggaaga gacgattaag tgtcagccgt tcgagtgtcc tgatcacttc
      481 tacgtaattg gcgggatgga tttcggatgg gatcacccac aggcgcaagt tcagctttgg
      541 tgggataagg aggcagacac aatctacgtt tcacgcgtgt ggaaggcgaa agaaaaaaca
      601 gccgttcagg cgtggggagc cgttaaatca tgggcgcata aagtgccaac cgcatggcct
      661 catgatggaa accagcacga gaagggcggc ggtgagcagc tcaaagggca gtatgcagac
      721 gctggattta tgatgttgca ggagcatgcg acatggcctg atggcggtaa cgctgttgag
      781 ccaggcatca ctgaattgcg cgacatgatg ctcgatggtc gcttcaaagt attcaatacc
      841 tgtgagccat tctttgagga gttccgcctc tatcaccgtg atgaaaacgg gaaaatcgtc
      901 aagcttaacg acgacgtgct atccgccgtt cgctatgcat acatgatgcg ccgcttcgca
      961 aaaatgatgc gcgacatcaa aaaaccaaaa gagaaaaaga taccagcccc aatcaggccc
     1021 atcgcacgga gaacttaaat ggccgacgaa aacagactca attccattct gtgtaagttt
     1081 gacgcagact ggatggcgag cgatgaagcc agaaccgagg cgacaaatga cctgtatttt
     1141 agccgagtgt cgcaatggga tgactggcta tcaaactaca ctaccctgca atatcgcgga
     1201 caattcgatg ttgttcgccc ggaggtcagg aaactggtcg cagagatgcg ccggaaccct
     1261 gtcgacgttc tcttccgacc caaagacggc gctaatcctg atgcagccga tgtattgatg
     1321 gggatgtatc gtactgatat gcgccataac acggcaaaga ttgccgttaa cgttggcgtt
     1381 cgtgagcaga tagagtcagg cgttggtgca tggcgtctgg tcacgcagta cgaagacaac
     1441 gatccaacaa gcaacaatca ggtaatccga cgcctgccaa tccatgaagc ctgctcacac
     1501 gtcatatggg acgccaacag caagcagatg gataagagcg acgctaagca ctgcacggtg
     1561 attaacgcct tgtcgcgcaa tggctggaaa gagttcgcag aggattacgg tattgacccg
     1621 gataccctgc catctttcca gaatcctaac gatacatggc tattcccgtg ggtgtcgaat
     1681 gatgtcgtct acgtcgctga gtattacgag gtagaagagg agaaggagaa ggtcttcatc
     1741 taccgcgacc cgctgacagg tgagccggtc agctattacc agcaggatat caaagacgtc
     1801 atcgacgacc tggctaatcg tggattcatt aaggtagcag agcgcaaggt gaagcgtcgg
     1861 cgtgtgtata agtcgatcat cacctgtacg cagatactga aagaccgcga gaagatagcc
     1921 ggagagcata ttccaatcgt tccagtgtat ggcgaatggt cattcgctgg tgacaaggag
     1981 cgctacgagg gtgtggtaag gctgacgaaa gacggtcaac gccttcgtaa catgattatg
     2041 tcgttcaacg ccgatattgt tgctcggtca ccgaagaaga aaccgacctt cttccctgag
     2101 caaatcgaag gctacgaata catgtacggc ggaaatgatg actatccgta ctatctgcag
     2161 aacaggactg atgaaaacgg taacgacctg ccgattggtc caatctccta catggaaaac
     2221 cctgaagtgc cgcaagccaa cgcttacatg cttgaggctg ccaccaacgc agtgaaagaa
     2281 gtggctagtc ttggcgtgga tgcgcaggcg gcaaatggtc aggtcgcttt tgataccgtc
     2341 aatcaactga acatgcgggc agaccttgag acatacgtgt ttcaggataa cctggctacc
     2401 gcaatgcgac gtgatggcga gatttatgcc tcaatggtca acgatattta tgacgttcct
     2461 cgtcatgtaa cgctgacact tgaagatgga agcgagaaag acgttcaact ctatgcgcaa
     2521 gttgtcgatt accagtccgg caatgtggtc acactcaacg acattcgcgg tcgatatgag
     2581 tgctatacag acgttgggcc atccttccag agtatgaagg aacagaaccg cgcagagatt
     2641 caggagttgc tcaccaaggt tccgcaaggt actccagagt tccagatgct gatgctgcaa
     2701 tacttcacgc tgcttgacgg taaaggcgtc gagatgatgc gagagtacgc gaacaagcaa
     2761 ctggtgatga tggggctgaa gaaaccagaa acacctgaag agatggagat ggtgcagcag
     2821 gcacaacaac agccgcagca gccatcagca gagcaaattc aggcgcaggg tatccttctg
     2881 caaggtcagg ctgaattgct caaggcagag aaccaacagg cgcagattca ggttgaagct
     2941 gccaaggttg aagcccaaaa ccaactcaac gccgcgaaga ttgcagaaat attcaacaat
     3001 atggacctcg acaagcaggc agaactgcgt gagtacctca ggctcgtagg tcaattccag
     3061 caacagcgca gcaaagatgc tcgtgctaac gctgagctgc ttcttaaaga tgcagaccag
     3121 actcattcac aacgcatgga tttcgcgaat cttatgcgtc aagttcaaat cccctccggc
     3181 ggagtagccg agacacctca ataagagaga gttaatcatg gaccaaacca ccgacattca
     3241 ggcttctgaa gaattaaccc tgcccggcaa tcatgcagcg gcatctgctg atggcttagt
     3301 tgtcgataat gccaacgaca gcgcaggtca ggaagaaggc tttgagattg tccggaaaga
     3361 cgatgagaaa ccaaaacaag acccggcaac taatgctgaa tttgcccgtc gccgcatcga
     3421 acgcaaacgc cagcgtgagc ttgagcagca gatggaagcg gttaagcgtg gagagttgcc
     3481 ggagcacctg cgggtgaacc ctgagttacc aaaacaacca gaccctaacg attatctttc
     3541 cgaagatgca ctggctaagt acgactatga ccagagccgc gcactggctg ccttccagca
     3601 ggcaaacagt gaatggcaga tcaaggctat ggacgcacga agccaggctg tcgccgagca
     3661 gggtcgcaaa actcaggagt tcacccagca atcagcgcaa tacgtcgagg cggcccgtaa
     3721 gcactacgag gcagcggaaa agctcaatat ccctgactat caggagaaag aggatgcatt
     3781 catgcaactg gtgccgccag cagtcggtgc cgacatcatg cgcctcttcc cggagaaatc
     3841 cgccgctctc atgtatcacc ttggtgctaa tcctgagaaa acacgccagt tgctggcgat
     3901 ggacgggcaa tccgcgctga ttgaactcac tcgactgtca gaacgtttaa ctctcaagcc
     3961 tcgagccaag cctgtttcag aagccccgtt acctgatgaa cccattcagg gacatgctgt
     4021 tgctgcaaat atctctgcga ttgaaaagca gatggaggcg gcagcaaaca aaggggatgt
     4081 agagacgtac cgcaagctca aggcgcaact gaataaagga attcgataat ggcattaaat
     4141 gaaggtcaac tggtcacgta tgctctggat gaaatcatcg gaaccgtcca gaacctgacg
     4201 ccaatggcgt caaaagtgac aaaatacccc cctccggcag aatccatgca gcgttcaagc
     4261 aacaccgtgt ggatgcctgt tgagcaggaa gcgccaaccc agactggctg ggatttaact
     4321 ggcaacgcaa ccgggattct ggaactctcc gtgaagtgca acatgggcga tccggataac
     4381 gatttcttcg agcttcgtgc agatgacctg cgtgatgagc gttcttaccg tcgccgcatc
     4441 caggcatccg ccaaaaaact ggcgaataac attgagtcag cgattgccaa acaggcaact
     4501 gaaatgggct cgcttgttgt tcacgatacc cgcgcaattg gtccatcgac tggcctgcct
     4561 ggctgggatt ttgtgtctga tgcagagcga ctgatgttct cccgtgagct aaaccgcgac
     4621 atgggcatca gttacttcct gaaccctgac gattaccgca aagcaggccg caacctggta
     4681 gatggtgaca tcttcgggcg cgttcctgaa gaagcatatc gaaacggtac tattcagcgt
     4741 cagattgctg gctttgatga aattcttcgc tcaccgaaac ttccggcagt taccaagtca
     4801 accgcgactg gtgtaactgt ttctggtgcg cagaagttta agccgcaggc atacaccctt
     4861 gataccgatg gtaacaaaga gaacgtcgac aaccgtgttg caacggtgac cgtatcctcc
     4921 accaccggat ttaagcgcgg cgacaaaatc agcttcattg gtgtgaagtt cctgtctcag
     4981 atggcgaaga atgtgctgac tgatgacgcg actttctcaa tcacccgtgt gatcgatggt
     5041 actcacatcg aaatcacgcc gaagccgatt gcgctggatg acgcgtcact gacaaaagaa
     5101 gagaaggctt acgctaacgt aaacacctct cttgctgata ccactccggt aaacgttctg
     5161 aacgtggcaa caaccaccgc taacgtgttc tgggctgatg actcaatccg cctgctgtct
     5221 cagccgatcc cggtaaccca tgaactgttt gctggcatga aaacgtcttc cttcagcatt
     5281 ccaggtattg gtgttaacgg catcttcgca acgcagggtg atatcaacac tctgtctggt
     5341 aagtgccgta ttgctgtgtg gtattcagca tgtgctgtac gaccagaggc aattggtgtt
     5401 ggtctgccta accagaccgc gtgataacca gagggagctt cggctcccgt ttttatctgg
     5461 agacaagcat gacacacatg atctttcgtc atggcgacat gaagaagtgg aaaggcgttg
     5521 gctacgactt tgaaatcgtg aaagccgaag agcttcagga atatctggat gctggctggt
     5581 tttcacatcc tgatgacctt ttgaaggatg ttgcagagcc agagccagaa gaaaaacagc
     5641 gtaaaaagcc tggtcgaaaa cctaaggcgg cagcagatga acctgacaac gaagggtgat
     5701 ttagttcttg cggcattacg taagctcggt gttgcatcaa atgccacgtt aaccgatgtc
     5761 gaaccgcagt ctatggaaga cggcgtcaac gaccttgaaa tgatgatggc tgaatggctt
     5821 ggcggtgatg cgtcacctgg tatcaacgtt ggctacattt ttgctgatgc agatgttgct
     5881 ccggatccgg gcgatgagca cggtttatca aataacgcta tcaatgccgt cattttcaac
     5941 cttgcctgcc gaattgctcc agattatgcg ctggaagcgt ctgcaaaact tataaccact
     6001 gccagatacg ggaaagagcg actcgtcaaa ctgtctgcaa tggacagggc aaaagcagct
     6061 aaatgtaagt ccggttatcc aaaccgtatg cctgttggta gtggtaacca gttggcgaag
     6121 tggaatggtt ggaattactt ccaccgaaag gaaccttgcg ataacgggag cgaataatgc
     6181 ctattcagca acttccgctt atgaaaggtg tcggcaaaga cttccgaaac gccgactata
     6241 tcgactatct gccagtgaat atgctggcta cacccaaaga aatcctgaac agcagcggat
     6301 atcttcgctc attcccgggc attgccaaac gttctgacgt gaacggtgta tctcgcggcg
     6361 tcgagtacaa catggcgcag aatgctgttt atcgcgtgtg tggcggcaag ctgtacaaag
     6421 gagaaagtga agtcggtgat gttgccggaa gtggtcgtgt atcaatggcg catggtcgga
     6481 catcacaggc ggtaggcgtt aatggtcaac tggtcgagta tcgctatgat ggcacggtca
     6541 aaaccgtctc aaactggcct acagacagtg gattcactca gtatgagtta ggttcagttc
     6601 gcgacattac gcgcttacgt gggcgttatg cgtggtcaaa agacggaact gattcatggt
     6661 ttatcactga ccttgaagac gaatctcatc ctgaccgtta cagcgcacaa tatcgtgccg
     6721 agtctcagcc tgacggcatc atcggtatcg ggacatggcg agacttcatc gtctgctttg
     6781 gttcatcgac tattgaatat ttctccctga ctggcgcaac caccgttggt gccgctttgt
     6841 atgttgcaca accatcgctg atggtgcaga aaggcattgc cggaacttac tgcaaaacgc
     6901 cattcgctga ttcgtatgcg tttatcagca atccggcaac gggtgcgccg tctgtgtata
     6961 tcatcgactc cggtcaggtg tcaccaattg ccagcgcgag cattgagaaa atcctccgct
     7021 cctacactgc tgatgaactg gctgatggtg tgatggaatc gctgcgattt gatgctcatg
     7081 agttgctgat tatccacctt ccgcgtcacg tcctcgtgta cgacgcatct tcaatcgcca
     7141 atggcccaca atggtgtgtg ctgaaaacag gtctgtatga cgatgtgtac cgcgctatcg
     7201 acttcgttta cgaaggcaat cagataacgt gcggcgataa gctggaatct gttaccggga
     7261 aactgcaatt cgatatcagc agccagtacg acaagcaaca ggaacacctg ctgtttactc
     7321 cactcttcaa agcggataac gccagagtgt tcgaccttga ggttgaatcg tcaactggcg
     7381 ttgcgcagta cgccgaccgc ctttttctct ctgcaaccac tgactgcatc aattacgggc
     7441 gtgagcagat gattgagcag aatgaaccgt tcgtttacga caagcgtgtt ttgtggaagc
     7501 gagtagggcg catcaggaaa aatgtcggct tcaaattgcg cgttatcacg aagtcacctg
     7561 tcactctgtc tggcgctcag ataaggattg agtaatggct gattcgaatc tcaatgtgcc
     7621 ggtaatcatc caagctacgc ggctcgatac atcagtcctt ccacgcaata tcttctcgca
     7681 gtcgtatctg ctttacgtta tcgcacaggg cactgatgtt ggtaacgtgg ctaacaaagc
     7741 caacgaggcc ggacagggcg cttatgatgc acaggtcagg aacgatgagc aggatgtgat
     7801 tctcgctgac catgagcagc gaatttctgc tgcggaagca acgcttgtta atcatgagga
     7861 gcgaatcaga caggcagaat caactcttca ggaccatgaa acacgaatag ctcagaatga
     7921 aagcgatatt tcgtcgcttg acacaagagt tcagtcgctg gagtcgcagg tttcagacca
     7981 tgaaacgcgc atcgatgctc tggagtatgc aaccacacgc aagaagtcag aggttgttta
     8041 ctctggtgta tctgtaacca tcccgacagc gccgaccaac cttgttagcc tgctgaaaac
     8101 gctcacaccg tcatccggct cgttggcacc attcttcgac accgttaaca acaagatggt
     8161 tgtgttcaac gagaacaaaa ccctgctctt caagttgtcg attgtcggca cgtggcccag
     8221 cggaaccgcc aacaggtcaa tgcagctaac cttttccggc tctgttcctg acacactggt
     8281 cagcagtcgt aatgcggcga caacaaccga caacatcctg ttagctacgt tcttcagcgt
     8341 ggataaagac ggctttcttg ccacaaatgg cagtacgtta accattcagt cgaatggtgc
     8401 ggcgtttact gccacaacca tcaagataat cgcggagcag tgatgattac attcaaacca
     8461 acgcgaaaca tcgacctgat agaagcagtc ggaaatcacc ctgacattat cgccgggagc
     8521 aacaacggtg atggatacga ctacaaacct gaatgccgtt actttgaggt gaacgtgcac
     8581 ggtcagtttg gcggcattgt ttactatcag gagattcagc cgctgacatt agattgccac
     8641 gccatgtacc tgccagagat tcgcggcttc agcaaggaaa ttgggctggc gttctggcga
     8701 tacattctga ctaacaccac cgttcagtgc gtaacatcgt tcgctgcacg caaattccga
     8761 cacggtcaga tgtactgcgc aatgattggc cttaagcgtg taggaaccat caagaaatac
     8821 ttcaaaggcg tggatgacgt gactttttac agcgccacac gcgaagaact aatcgacttc
     8881 ctgaatcacg ggagatagcc atgttatatg catttaagct gggcagaaaa ctgcgcggcg
     8941 aggaacctta ttgccctgaa aaaggcggga aaggtggcag ttctgataaa agcgcaaagt
     9001 atgcagcaga agcccagaag tatgctgcag acctgcaaaa tcagcagtgg cagacgatca
     9061 tgaaaaacct tgctccgttc acgcctcttg cggagcagta tgttaaccag cttcagaacc
     9121 tttccagttt agaaggtcag gggcaggcac ttaatcagta ttacaactct aagcagtata
     9181 aagaccttgc agggcaggcg cgttaccaga gtcttgctgc tgcggaggcg acgggtggac
     9241 ttggttcgac agccacaagc aatcaactgg ctacgatcgc gccgacactc ggtcagtctt
     9301 ggttatcaaa tcagatgagc aattacaaca atctggcaaa cgttgggctt ggtgcgctgc
     9361 aaggtcaggc aaacgccggg cagacgtacg ccaacaacat gagcagcatt gcacagcaaa
     9421 gcgcagctct tgccgctgct aatgccaata aaccatcaag tcttcagact gcaattagcg
     9481 gtggcacgtc tggtgcgatt gccggtgcag gtcttgccag ccttttggga acatcaacgc
     9541 cttggggcgc tggtattggt gctggtatcg gattgcttgg ctcgttgttt taagggggaa
     9601 tcatggctac ttggcaagga acaaacggcg gattgttggc tggtatcggc ggcgtcaact
     9661 caaacgctcc gagcgtaaat gacatcggca atacgcttca gcttatcagg cagaacaatg
     9721 atattgagcg ttcaggcgct aacaatgttg ggctgactgc tttgcaaggt ctttcaggta
     9781 ttgcgggtgt ttttcagcag gaaaagcagg ctcagcggca gaaagaattt cagcaggcgt
     9841 acgctaatgc ttatgcgtct ggtgatcgcg gtgctttgcg tcagttggct actcaatatc
     9901 cagaccagat tgaatccgtt cgtaaaggca tgggattcat tgatgaagag cagcgtaatt
     9961 ctatcggcac cttagcggct ggcgcacgcc ttgcgtcatc gtctccagaa gcaatgcaat
    10021 catggctgca aaacaacgcc ggtgagttag ctcgtgttgg cgttaatcct catgacgtcg
    10081 ctcagatgta ccaacagaac ccgcggcagt tcggcgaatt tgtcgatcac ctggggatga
    10141 acagtctcgg gcccgaaaaa tactttgacc tacaggataa aatgcagggt cgccaggtta
    10201 ccatgcgcgg tcaggatctg gattcgcaaa ccgccgctcg gaatcaggca atcacaatgc
    10261 gcggacaaga tatccaggcg aatttaggtc agcagcgcat taatctggac gcagaaacaa
    10321 accgcattaa caacgaaaat aagcgccttg accggatgct atcagcagaa actaacgacc
    10381 tgaagcgcca ggaaatacag agccgcatag cagccaacaa ccagcagttg cagcagaagc
    10441 agcaagcgct aaatgatggc tacaaagacg gcatcaacac cctcacaacc agcatgttca
    10501 ctctgaacga tatcgttagt tctccttcac ttaagagcat tacaggctta cgtggagtaa
    10561 tccccaacgt tccaggctca caggctgcag acactcaggc acgacttgat acctttaaat
    10621 cacaagcata cctgacagcg gttcaggcca tgcgaggcat gggcgcactt tctgatgccg
    10681 agggcaaaaa gctcgaccag gctgttggtt cgctgcagaa ctcgcagagc gaggagtcct
    10741 ttcgtcgcaa cgctggcgtc atcctgaaca cgctcaacca gaagcgtaat gaggcggttg
    10801 gtaagtacgt tcagcaaaac ggtatcaagc gagtggaagc gcctcaggct tctatagatt
    10861 acctgaagca gcaccccgag ctgtcaatcg acttcattaa tcgctacgga tatcttccat
    10921 ctttggggca gtaaatggct aattaccgtg atttgttaga gcaggctggc gcacgttacg
    10981 gtgtgccaga agggttgatg actgcactgg gtgccaagga gtcttcttac aaccctgccg
    11041 cagtaagctc cgccggggct gtaggattga ctcaggtcat gcctgggaca tggcgtgata
    11101 tgggttatac cgatgagcaa atgcaaaacc ccgaatatca ggctgacgct ggcgcgcgct
    11161 atctggcaaa gatgtaccag cagtttggta actggcgtga ctctcttcag gcttatcacg
    11221 acggtcccgg caacgttatg aaggcaaagc gtggtgaata tacgccagga cctgaaggcc
    11281 gcggttacgt tgatgatcgc tttgctcaat gggctggtga cccggtgaca gactcaacag
    11341 tcgaacagcg cgccacctct gcaaaggtac atcctcagca agaccctaac aacccgtttg
    11401 cacaactgga agtacagtca tccgaacaag tatcggcatc aggtgtgcag tcagacccaa
    11461 ataatccatt tgctcagatt gagcagcagg cagccagtca gcagccacct caacccgtaa
    11521 gttctgtcgc accgaaacct gttcagcaac aaacagttaa tcaggccaat aatgcaccaa
    11581 cacgggaaga accatcattg atgcaacaag ctggcgattg gctcactggt ggtcaaagtg
    11641 cagggcaaat tgcagaacag gctggtcgtg gtctggtaaa cataccattt gacgtattgc
    11701 agggtggcgc aagtctgatt aatgcaatca gtcaggggct tggggggcca aaagtattgg
    11761 atgatgttta tcgtccagta gacagaccga cagaccccta cgcgcaagct ggagagtcaa
    11821 taggcggtta tcttgttcct ggagcaggtg tagctggaaa catggtcatt ggttctctcg
    11881 ctgacgcggc gaatcaacgg ggtgattttg ccgaaaatgc cgctattaat gccggactta
    11941 gcattgctac gcatggcctg ataaatggcg ttacccgtgg tgttcgtggt gcatcaaata
    12001 taattagtgg caataaaaca tctgcacaga gagcgaccac tgcgccaaca gaaacatcac
    12061 cattctccgg tgatgccgct gcagcaacaa atcctgcggt tcatgccgca gaggcaagag
    12121 tagcacaagg tgtaccaatg acgcctgcga cgaagaaccc agaggaagtc gttcgcacag
    12181 tagcagcaca aaaaaggcca aatctcgctt catcgcttga tgaactagat atcaatcctc
    12241 aggctgaagt tctggagtct gctgaaaggc ttaatgttga ttcattactc ccttcacact
    12301 tttccgggaa cgagcaatac aaggcagttg agcaagcaat caagtcccgt gcgggttctg
    12361 ctctacaggt gcaggaaaat gaagcaatca ggcagctagc acagggcgcg ggtgagataa
    12421 ttgatcgcgt ttccggtgca aaagatgctc ttggtatgag cgacaagttt attgatacgg
    12481 tcaatggaag aatgtctgcg ctgatgaaac gaagcgacca gctttatcgc aatgttgaaa
    12541 aggcgatgcc tgcaggtgca aaaattgatg cgccatcaac aaggtcaatg ctcaaacagg
    12601 tggcagaaga tcttggcggg atgaaaaacc ttgaccctat tgaaaagaga gttgaggtag
    12661 cctgagttta acggacactc cttcctgaaa tagaatggca tcagaaggag ctaataatga
    12721 gcagaaaaac cca
//
LOCUS       DAZPCS010000105        12389 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZPCS010000105 DAZPCS010000000
VERSION     DAZPCS010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12389
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.105"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            327..1967
                     /gene="trg"
                     /locus_tag="ACHUQ7_002841"
     CDS             327..1967
                     /gene="trg"
                     /locus_tag="ACHUQ7_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFR1136161.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEAFDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(2005..2928)
                     /locus_tag="ACHUQ7_002842"
     CDS             complement(2005..2928)
                     /locus_tag="ACHUQ7_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR1136162.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSTSLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            3145..4488
                     /locus_tag="ACHUQ7_002843"
     CDS             3145..4488
                     /locus_tag="ACHUQ7_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR1136163.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQLATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            4713..6368
                     /gene="opgD"
                     /locus_tag="ACHUQ7_002844"
                     /gene_synonym="mdoD"
     CDS             4713..6368
                     /gene="opgD"
                     /locus_tag="ACHUQ7_002844"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFR1136164.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGISP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            6508..6732
                     /locus_tag="ACHUQ7_002845"
     CDS             6508..6732
                     /locus_tag="ACHUQ7_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFR1136165.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            6795..7331
                     /gene="rimL"
                     /locus_tag="ACHUQ7_002846"
     CDS             6795..7331
                     /gene="rimL"
                     /locus_tag="ACHUQ7_002846"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFR1136166.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQTLIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(7326..8306)
                     /gene="ydcK"
                     /locus_tag="ACHUQ7_002847"
     CDS             complement(7326..8306)
                     /gene="ydcK"
                     /locus_tag="ACHUQ7_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFR1136167.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYVQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            8430..9422
                     /gene="tehA"
                     /locus_tag="ACHUQ7_002848"
     CDS             8430..9422
                     /gene="tehA"
                     /locus_tag="ACHUQ7_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFR1136168.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            9419..10012
                     /gene="tehB"
                     /locus_tag="ACHUQ7_002849"
     CDS             9419..10012
                     /gene="tehB"
                     /locus_tag="ACHUQ7_002849"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFR1136169.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLTRKK"
     gene            10315..10971
                     /locus_tag="ACHUQ7_002850"
     CDS             10315..10971
                     /locus_tag="ACHUQ7_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HFR1136170.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYNNYSDLKETTSAT
                     GKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMKEAIA
                     QRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTMDTRL
                     YFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFDVNKK
                     "
     gene            complement(10989..11495)
                     /gene="tnpA"
                     /locus_tag="ACHUQ7_002851"
                     /pseudo
     CDS             complement(10989..11495)
                     /gene="tnpA"
                     /locus_tag="ACHUQ7_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            11503..12389
                     /locus_tag="ACHUQ7_002852"
                     /pseudo
     CDS             11503..12389
                     /locus_tag="ACHUQ7_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
BASE COUNT         3231 a         2903 c         3109 g         3146 t
ORIGIN      
        1 tgatagaaat atcggtcggg gccttcgtct ttctgattcc cggttagcct gaaaacagaa
       61 agtctcaggc acccgcaggc atcctatgag gtttccttag ggacgaaaat aatcacttca
      121 cgaaattgcg tgctgttttc cagaattttt cgtcattcgg gttagccagt ttagccattc
      181 gttactctct tcattccaat agcattaatt ttctctgcaa taattgttgt aaaaatgtga
      241 cgcaaagagg tttttggtca taagtaatta ccgtcaagtg ccgatgactt tctatcagga
      301 gtaaacctgg acgagagaca acggtaatga atacaactcc ctcacagcga ttaggttttt
      361 tgcatcacat caggctggtt ccgttatttg cctgcattct tggcggtatc ttagttctat
      421 tcgcattaag ttctgctctg gctggctatt tcctctggca ggccgatcgc gatcagcgtg
      481 atgttactgc ggagattgag attcggaccg ggttagcgaa cagttcagat tttttgcgtt
      541 cagcccggat caatatgatt caggccgggg ctgcgagtcg tattgcggaa atggaagcaa
      601 tgaagcgaaa tattgcgcaa gccgaatcgg agattaaaca gtcgcagcaa ggttatcgtg
      661 cttatcagaa tcgaccggtg aaaacacctg ctgatgaagc cttcgacact gaattaaatc
      721 aacgctttca ggcttatatc acgggtatgc aacctatgtt gaaatatgcc aaaaatggca
      781 tgtttgaagc gattatcaat catgaaagtg agcagatccg accgctggat aatgcttata
      841 ccgatatttt gaacaaagcc gttaagatac gtagcaccag agccaaccaa ctggcggaac
      901 tggcccatca gcgcacccgc ctgggtggga tgttcatgat tggcgcgttt gtgcttgccc
      961 tggtcatgac gctgataaca tttatggtgc tacgtcggat cgtcattcgt ccactgcaac
     1021 atgccgcaca acggattgaa aaaatcgcca gtggcgatct gacgatgaat gatgaaccgg
     1081 cgggtcgtaa tgaaatcggt cgcttaagtc gtcatttaca gcaaatgcag cattcactgg
     1141 ggatgacagt agggactgtt cgacagggcg cggaagagat ttatcgtggc accagcgaaa
     1201 tttcagctgg caatgcggac ctgtcatctc gcaccgaaga acaagcggcg gctatcgaac
     1261 aaactgccgc cagcatggag caactcactg cgacggtgaa acagaatgcg gataacgcgc
     1321 atcatgccag caaactggcg caagaggctt ctattaaagc cagcgatggc gggcagacgg
     1381 tttccggtgt agtaaaaacg atgggcgcta tctccacgag ttcgaagaaa atttctgaga
     1441 tcaccgccgt catcaacagt attgctttcc agacgaatat tctggcactg aatgctgccg
     1501 ttgaagccgc gcgagcgggt gagcaagggc gtggatttgc cgttgtcgcc agcgaagtac
     1561 ggacactcgc aagccgcagc gctcaggcag cgaaagagat tgaaggcttg atcagtgaat
     1621 cagtcaggtt aattgacctg gggtcggatg aggtggcaac ggcagggaaa accatgagca
     1681 ctattgttga tgccgtcgcg agtgtcacac atatcatgca ggaaatcgcc gccgcctcgg
     1741 atgaacaaag tagaggcata actcaggtta gccaggcgat ttctgaaatg gataaggtga
     1801 cgcaacagaa tgcttctctg gtagaagagg cctcagcagc ggcggtgtcc cttgaagaac
     1861 aggctgcacg attaactgag gcggtgaacg tattccgtct gaacaaacat tctgtgtcgg
     1921 cagaacctcg cggagcgggt gaaccagtta gtttcgctac ggtgtgaaaa tgttcaagga
     1981 gggatcgaca gatcccttca cctttcagaa cggcattgat tttcgaatag cgttaatcat
     2041 caactggcaa ccagaagaga acgtcgcatc tacgcgggtc agtattccaa tcggttcgcc
     2101 tgcaccatgt cccggaacag gcagggccac cagcgtggca tgacgcaggt cgtcttttac
     2161 agcgccagaa gggacaaacc acacgtaatc gtattcaacg gtaagttgac gagatagcga
     2221 agtagacagc gtttcgatac aacccgaagg aattttacag ccctggctct gcactaatgc
     2281 atctgaatgc tggcgtggcg cagtgccttc tggtgataca acgaccggcc attccagcac
     2341 ccggcttagc gttacgttct cctgaagtag cgggtgatta gggcggacaa ccagcttcaa
     2401 cgattcaaga aacagcagtt cgtaattaag cccggtcatc agttcaggat ctgacatccg
     2461 accaatgccg atatcgattt ccccggtttt taaacccgcc agaatcatag ggttactcat
     2521 tgtcgcaact tgcaaggtcg tctctttttg ttgctgatga aactgaccta taaccgaagg
     2581 taatatcccc agtgccgcag taggtagtgc accaaccctg acgacatcat tattaagacc
     2641 ttctttacga tgaagcgact gtccggcagt gttgatggcg tcaagaactc tgactgcatg
     2701 cgttaaaaat tgttcgccgg gtaaggtaag ttgcgccccc tgacgaccac gctcaaacaa
     2761 gcgagcgccc gtcagctgct ccagttcatt caatgtatta gagagcgcag gttgactcaa
     2821 attaagggtt tcagctgcgc gccccaaagt tccttgttgt gcgacagcta cgaatgtatg
     2881 aaggtggcgc aaacggatgc gctgactaaa cagactattt ttttccataa gcgatgttaa
     2941 aaacgaagcg gtgtcgctga caagtgaagt tgtttgatta tgataacttg attgcaaaat
     3001 attattaaca attaaagcaa ttatgttaca gcaaaatgga taatattgat gttttcgcgg
     3061 cgagatcaca gtttgtaaat tcttcccgcc agagtgaatg cggttaccta cactccagat
     3121 tactgaccac tggaggcaga cactatggcg aacagcatca cggcggatga gattcgggaa
     3181 cagttttcgc aggcaatgtc agccatgtac cagcaagaag ttccgcaata tggcacgctg
     3241 ctggaactgg tagctgatgt gaatctggct gtgctggaaa acaatcctca actgcacgaa
     3301 aaaatggtaa atgcagacga gctggcgcga ctgaatgttg aacgtcatgg ggcgattcgc
     3361 gttgggactg cacaagagct tgctactctt cggcggatgt ttgccattat ggggatgtac
     3421 ccggtgagct attacgatct ctcgcaggca ggggtgccgg tacattcgac agcatttcgg
     3481 cccattgatg atgcttctct ggcgcgtaat cccttccgcg tttttacatc cttactccgc
     3541 cttgagctta tcgagaacga aattttgcgc cagaaagcgg cggagattct gcgtcagcgc
     3601 gatatcttca ccccacgttg tcgacaactg ttagaggaat atgagcagca gggcggtttt
     3661 aacgaaacac aggcacagga gtttgtgcag gaagccctgg aaacgtttcg ctggcaccag
     3721 ttagcaacgg tagatgaaga aacctatcgc gccttgcaca acgaacatcg gttgattgct
     3781 gatgtggtct gttttcctgg atgccatatc aaccacctga cgccacgtac gctggatatt
     3841 gaccgggtgc agtcgatgat gcctgaatgc ggaattgaac ccaaaattct catcgagggg
     3901 ccgccgcgcc gcgaggtacc gattttacta cgccagacca gctttaaagc actggaagag
     3961 acggtgttgt ttgcggggca gaaacagggc acgcataccg cgcgctttgg tgaaattgag
     4021 cagcgtggcg tggcattaac gccgaaaggg cgacaactgt atgatgatct tctgcgtaac
     4081 gctggaaccg ggcaggataa tctcactcac caaatgcatt tacaggaaac cttccgcact
     4141 tttcctgaca gtgagttttt aatgcgtcag caagggttgg catggttccg gtaccgtctg
     4201 acgccttcgg gtgaggcgca tcgtcaggcg attcatcctg gagacgatcc acagccctta
     4261 attgaacgtg gttgggtagt ggcgcaaccc atcacctatg aagatttctt gcccgttagc
     4321 gcggcgggga tcttccagtc aaatctgggt aatgaaacgc agacacgcag tcacggtaat
     4381 gccagtcgcg aagcatttga gcaggcgttg ggttgtccgg ttttggatga gttccagctt
     4441 taccaggaag cggaagaacg cagtaaacgt cgctgtggtt tgctttaaat tctgaccatc
     4501 cgcctttgca aaaatttgcc tgattttaca aacgaatcag gctcatccca tcgacataaa
     4561 aaaaatgccg atttatgcat attctctcag ttcaacaatt ggattattaa taaatattgt
     4621 ctagagtgag cggtcataaa taagcacttt cttgccgctg aaaacgacca gcgcgggacc
     4681 attcacaaca ccagaaggac tcactttcag gtatggatcg tagacgattt attaaaggtt
     4741 caatggctat ggccgccgtg tgcggtacca gcggcattgc ttctcttttt tctcaggcag
     4801 cattcgcggc agattctgat attgccgacg ggcaaaccca gcgttttgac ttctccattc
     4861 tacagtcaat ggcgcacgac ttagcgcaaa cagcgtggcg tggtgcgcct cgtccgttac
     4921 ctgacacgct ggcgacaatg acgccgcagg cttataacag tattcaatac gacgccgaaa
     4981 aatcgctctg gcataacgtt gagaaccgtc aactggacgc tcagttcttc catatgggaa
     5041 tgggattccg tcgccgcgtt cgtatgtttt ctgtagatcc agcaacacat ctggcgcgtg
     5101 aaattcactt tcgcccggag ttgttcaaat acaacgatgc aggtgttgat accaaacaat
     5161 tagaagggca aagcgatctc ggctttgccg gttttcgcgt gtttaaagcc cccgaactgg
     5221 cgcgccgtga tgtagtatca tttctcggcg cgagttattt ccgcgccgtt gatgatacat
     5281 atcaatacgg tttgtcggcc cgcggcctgg cgatcgacac ttacaccgac agtaaagaag
     5341 agttccccga ctttaccgcc ttctggtttg atacggtaaa accgggggca actaccttta
     5401 ccgtttatgc gttgctcgat agcgccagca ttactggtgc ctataagttc actatccatt
     5461 gtgagaaaag tcaggtgatt atggatgtgg aaaatcacct gtatgcgcgc aaagacatta
     5521 aacagctggg catttcgccg atgaccagta tgttcagctg cggtactaat gaacgtcgga
     5581 tgtgcgatac aattcatccg caaattcatg actctgatcg tctgtccatg tggcggggca
     5641 acggcgagtg gatttgccgt ccgctgaata atccgcaaaa attgcagttc aatgcttaca
     5701 ccgacaacaa cccgaaaggg tttggtttat tgcaactgga tcgtgacttc tcccattatc
     5761 aggacattat gggctggtat aacaaacgcc caagtctgtg ggtggaaccg cgtaacaagt
     5821 ggggtaaggg caccatcggc ctgatggaaa tcccaacaac gggcgaaacg ctggataaca
     5881 ttgtctgctt ctggcagcca gaaaaagctg taaaagcagg tgatgagttt gcattccagt
     5941 atcgtctgta ctggagtgcg caaccgcctg ttcattgccc attagcgcgc gttatggcga
     6001 cgcgtaccgg catgggcggt ttcccggaag gttgggcgcc aggtgaacac tatcccgaaa
     6061 aatgggcgcg tcgttttgcc gtcgatttcg ttggtggtga tctgaaagct gccgcgccaa
     6121 aaggcattga gccggtgatt acgctttcca gtggggaagc gaagcaaatc gaaattctct
     6181 atattgaacc catcgatggt tatcgtattc agtttgactg gtatccgact tcggactcca
     6241 ctgatccggt cgatatgcgg atgtatctac gttgtcaggg ggacgctatc agtgaaacat
     6301 ggctgtatca gtatttcccg ccagcgccgg ataaacgtca gtatgttgac gaccgcgtga
     6361 tgagttaatc gttttttctt cggcaccttc ttcgggaggt gccgtctggt taaacacgat
     6421 cccgctcgca tttttcccta agttaaatga gtaatctgat ggtgtgtatt tcagatacac
     6481 cgtgtcagcc actaacaggg agtgcgtatg tttccagaat accgagattt aatatcccgt
     6541 ctgaaaaacg aaaatcctcg ctttatgtcc ttgttcgata aacacaataa acttgatcat
     6601 gaaattgcca gaaaggaagg ttccgacggt cgagggtaca atgcggaagt ggtccgcatg
     6661 aaaaaacaaa agctacagtt aaaagatgag atgctcaaaa tcctgcagca ggagagcgtc
     6721 aaagaggtgt aaactttcct aagccgccag ccaggcggct tttttaacaa cagcatggat
     6781 tgactggaga taagatgact gaaacgataa aagtaagcga atcacttgaa ttacatgctg
     6841 ttgcggaaag tcacgtcaca cctctttatc agttaatctg taaaaataaa acctggttac
     6901 agcagtcgct aaactggccg cagttcgttc agagtgaaga ggacacgcga aaaacggtgc
     6961 agggtaatgt gatgttgcat caacgcggct atgccaaaat gttcatgatt ttcaaagaag
     7021 atgaacttat cggcgttatc tcgtttaatc gtattgaacc actgaataaa actgctgaaa
     7081 tcggctactg gctggacgaa tctcatcagg ggcaggggat catttctcag gcgctgcaga
     7141 cattgattca tcattacgct cagtctggtg aacttagacg cttcgtgatc aaatgtcggg
     7201 tggacaatcc gcaaagcaac caggtcgctt tgcgcaatgg ttttatcctt gaaggttgcc
     7261 tgaaacaggc tgagttcctg aatgatgcct atgatgatgt gaacttatac gcgcgtatta
     7321 tcgattcata ataaccctgc cagcggcgtt cgcgtaatgc gctcctcgcc gttaatgact
     7381 tttggaccac ggacatgaac cgtgtcgcca tcaaaggctt caacgacggc gtggtcggtt
     7441 aactccacat ggttttcaat tatcaccgca cctgtaatac ggctctcacc ttgtattaca
     7501 acgtgctcgt cgagcagaat cggtccacca cgtaccacgg catttccgcc aatcaagaca
     7561 tgatgtttta acacacaatt accttccaca atggcatatt ccgccacctg cgaactgtaa
     7621 tgaatcgtcg gaatggcatc ttcttctatg ccagctttca cctgcgcgtg accgtagact
     7681 ttagcgcaat cgcatagcca gacgttgttc tcttcattac cttcaacact ggcaaaatca
     7741 aaaacttcgg cgcgatgttc aataaaagca taccggacga cggcatcgcc ataaatttgt
     7801 gcctggtgga caatacgcga ggcgctaacc cttgcgcgat cataaatttg gagcaggagt
     7861 tgatggtcgg gcgttaagcc ttgtgcggcg acgatcatag aatgttgatc aattaaggcg
     7921 tgaccaaata ttctacattg cacgtaaact aatgagtcat gaatggtgac actgtcactt
     7981 atatatgcac cctggctgat ttcactatta tcgatccaga cgttatccgt tgcgtaaacc
     8041 tctccccaca gcacgctggt gccggtgata cgcgtattac cggaaatcac cgttccgccg
     8101 aacgcaatag cattctggtc gtaaatccag caatttcctt cctgtgctaa caccgtttcg
     8161 cgatcgatcc agccgccagc ggttcctgct atcacatcgt taaaatcgct aatggcgata
     8221 atctgccgta ataacacact ttttttagtg ccatcttcct gataactaaa ggctcgctgt
     8281 tcttcactta ggcgatattt gcgcatagcg ttttcccaca ggtgacttac tataaccgta
     8341 gcaaattctg cggctctggc tatgctcgag aaaattccat aaaatgcatt tcaaatatac
     8401 tttataaatt aaacaaaatg agtaagaaga tgcagagcga taaagtgctc aatttgccgg
     8461 caggctactt tggtattgtg ttggggacga tagggatggg atttgcctgg cgctatgcca
     8521 gccaggtttg gcaggtcagc cactggttag gggatgggct ggtgattctg gcgatgatca
     8581 tctggggatt attgactagc gcatttatta cccgactcat acgctttccg catagcgtgc
     8641 tggcggaagt tcgccatcca gtgctgagca gttttgtgag tttgtttccg gcaacgacga
     8701 tgctggtggc gattggtttt gttccgtggt ttcgcccact ggcggtgtgc atgttcagtt
     8761 ttggtgtcgt ggttcagttg gcttatgccg cctggcaaac tgcgggatta tggcgcggat
     8821 ctcaccctga agaagctacc acgcctggac tgtatctgcc gacagttgcc aacaacttta
     8881 tcagcgcaat ggcctgtggt gcgttgggct acaccgacgc cggtctggtg tttttaggcg
     8941 caggcgtttt ctcatggcta agccttgaac cggtgatctt gcagcgtctg cgcagttcgg
     9001 gagaattacc cacggcactg cggacatcac tcggcattca gctcgctcct gcgctggtgg
     9061 cctgtagtgc ctggctgagc gtcaacggcg gcgagggtga cacgctggcg aaaatgcttt
     9121 tcggttatgg actgctgcaa ctgctgttta tgctacgtct gatgccatgg tatctctccc
     9181 agccatttaa tgcttcattc tggagtttct cgttcggcgt atctgcactg gcaaccaccg
     9241 gtttgcatct ggggagtggc agcgataatg gatttttcca tacgctggcg gtgccgctgt
     9301 ttatctttac caattttatt attgcaatac tgctcatccg tacttttgcg cttctgatgc
     9361 agggaaaatt gttagtcaga accgagcgcg ccgttttaat gaaagcagag gacaaagaat
     9421 gatcattcgt gacgaaaact attttactga taaatatgaa ttaacccgca cacactctga
     9481 agtactggaa gcggtgaaag tggttaaacc gggtaaaacg ctggatctgg gctgtggcaa
     9541 tggtcgtaac agtctttacc tggcagccaa tggttatgat gttgacgcat gggataaaaa
     9601 tgccatgagt atcgccaacg tcgagcgcat taaatccatt gaaaatctgg ataatttaca
     9661 cacccgggtc gttgatctga ataacctcac atttgatgga cagtacgatt ttattctttc
     9721 gactgtggtg ctgatgttcc ttgaggctaa aaccatccca gggttgattg ccaatatgca
     9781 acgttgcact aaacctggtg gttacaacct gattgtggcg gcgatggata ccgctgatta
     9841 tccatgtacc gtcggcttca cgtttgcctt caaagaggga gaattacgtc gatattacga
     9901 aggctgggag agggtgaaat acaatgaaga cgtcggcgag ctgcaccgca ccgacgccaa
     9961 cggtaatcgt attaaactgc gtttcgccac gatgctgaca cgtaaaaaat gacccggtaa
    10021 gcacaaaacg cgtgaaaatt ccccacgctg agatgattta ctgttcttct tttcggtaag
    10081 catatttttt atcgaaggga tgtgaaatta atcacagtag tcgaagtttt ttagcagctt
    10141 aacttactga aatttaacta ctgatgattg acttagcccc tttttcggca ttgactatgt
    10201 cgtctgaaaa ggggctgaaa aatttatttt caccaacact ttttttgcca caacacgaag
    10261 cggcgctttt tgctataact tagaaagtaa tataatcatc tcaggaaact attcatgcgt
    10321 accacatcat ttgcgaaagt tgcagcttta tgcggcttgt tggctctgtc tggttgtgcc
    10381 tctaaaatca cccagccaga taaatataac aattactctg atttaaaaga aacaacctca
    10441 gcaacaggta aacctgtttt acgttgggta gacccgagtt ttgatcaaag caaatatgac
    10501 agcatcgtct ggaacccaat cacttattat ccggtaccaa aaccttctac ccaggtaggg
    10561 cagaaagttc tggataaaat tttgaactat accaacaccg aaatgaaaga agcgatagcg
    10621 cagcgtaaac cagtggttac caccgctggg ccgcgtagtc tgattttccg tggggccatt
    10681 accggtgtag ataccagcaa agaagggctg caattctatg aagtggttcc tgttgcatta
    10741 gtggttgcgg ggacgcaaat ggctacaggc caccgtacca tggatactcg cctctatttt
    10801 gaaggtgagc tgattgatgc agcgactaat aaaccggtta tcaaagtcgt tcgtcagggc
    10861 gaaggtaaag acctgaataa cgaaagtacg ccaatggctt tcgaaaatat taaacaagtt
    10921 attgatgaca tggcgaccga tgccaccatg tttgacgtta acaaaaagta gttccagacg
    10981 cgccatcgtt agatggcgct ttttatccgg tgcgccgtaa aaccccatcc ttcagggcgg
    11041 ggatataagg cgcggttttc cacctgacca ggtgtttgct gttgttcaat gtattggcgg
    11101 atgacggata ttggcgcacc tccgcagcta ctggcaaaat agccaggact ccacaaaacg
    11161 cctttgtagt aatacctgac tgcaatatct ggtcgatctc gtcgcagtaa cctacccgat
    11221 acgcctttga ggctgtttac cagactggat atcgccagtt tgggaggtta gttaattaac
    11281 aaatggacgt gatctggttc gccatccatt tcaaccagtt cagcttcaaa ataagcacat
    11341 acatttgaaa agtaagtgcg tagtttttct gtcgcgtcat ggtcaaaaat ctggcgtcgg
    11401 tatctggtga caaagactag gtgaacatgc atcaggaaaa cacaatgcct tccacgccgg
    11461 atatcggttt cttttttcac agaccaaagt atcatttgac ctgtgaaacg attacaggca
    11521 tttaaattcc agttaagacc aggtggtcaa caggagtgtg aaatgaggcg cttcgcaggc
    11581 gcatgtcgtt tcgttttcaa tcgtgctctg gcacgtcaga atgagaatca taaggccggt
    11641 aataaataca tcccttacgg gaaaatggct tcctggctgg ttgagtggaa aaatgccact
    11701 gaaacgcaat ggcttaaaga ttctccctca cagccattgc aacagtcact gaaagacctt
    11761 gagcgggctt acaaaaactt cttccggaag cgggctgctt ttccccgatt caaaaagcgg
    11821 tgacagaatg atgcattccg ctacccgcag ggcgttaagc tcgatcagga aaacagccgt
    11881 atttttctgc cgaaactggg ctggatgcgc taccggaata gccgtcaggt cacgggtgtt
    11941 gtgaaaaatg tcactgtcag ccagtcctgc ggtacgtggt acatcagtat tcagacagaa
    12001 agtgaagtat ccactccggc tcacccttca gcatcaatgg tcgggctgga tgctggcgtg
    12061 gctaaactcg ccacgctgtc agatggcaca gtctttgagc ctgtaaacag ttttcagaaa
    12121 aaccagaaga cgctggcgag acttcagcga cagttaagcc gcaaggtcaa attcagcaac
    12181 aactggcaga agcagaaacg caaaatacag cgactgcatt cctgtatcgc aaatatccgc
    12241 agggactacc ttcacaaagt cacaacggcc gtcagcaaaa accacgcaat gatagtcatt
    12301 gaggatttga aggtcagcaa catgtcaaag tcagcagcgg gtacggtcag ccagccgggg
    12361 cgcaatgtcc gggcaaaatc aggtttaaa
//
LOCUS       DAZPCS010000106        12342 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZPCS010000106 DAZPCS010000000
VERSION     DAZPCS010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12342
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.106"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            758..841
                     /gene="dinQ"
                     /locus_tag="ACHUQ7_002853"
     CDS             758..841
                     /gene="dinQ"
                     /locus_tag="ACHUQ7_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFR1136171.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(895..2247)
                     /gene="gorA"
                     /locus_tag="ACHUQ7_002854"
     CDS             complement(895..2247)
                     /gene="gorA"
                     /locus_tag="ACHUQ7_002854"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFR1136172.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(2319..3161)
                     /gene="rlmJ"
                     /locus_tag="ACHUQ7_002855"
     CDS             complement(2319..3161)
                     /gene="rlmJ"
                     /locus_tag="ACHUQ7_002855"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFR1136173.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            3364..5406
                     /gene="prlC"
                     /locus_tag="ACHUQ7_002856"
     CDS             3364..5406
                     /gene="prlC"
                     /locus_tag="ACHUQ7_002856"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFR1136174.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            5414..6166
                     /gene="rsmJ"
                     /locus_tag="ACHUQ7_002857"
     CDS             5414..6166
                     /gene="rsmJ"
                     /locus_tag="ACHUQ7_002857"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFR1136175.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(6215..7683)
                     /gene="dtpB"
                     /locus_tag="ACHUQ7_002858"
                     /pseudo
     CDS             complement(6215..7683)
                     /gene="dtpB"
                     /locus_tag="ACHUQ7_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
     gene            7849..7902
                     /locus_tag="ACHUQ7_002859"
     CDS             7849..7902
                     /locus_tag="ACHUQ7_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1136176.1"
                     /translation="MVLNFVLAGDFDCVRRS"
     gene            complement(8001..8435)
                     /gene="uspA"
                     /locus_tag="ACHUQ7_002860"
     CDS             complement(8001..8435)
                     /gene="uspA"
                     /locus_tag="ACHUQ7_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFR1136177.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            8829..9164
                     /gene="uspB"
                     /locus_tag="ACHUQ7_002861"
     CDS             8829..9164
                     /gene="uspB"
                     /locus_tag="ACHUQ7_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFR1136178.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(9235..10734)
                     /gene="pitA"
                     /locus_tag="ACHUQ7_002862"
     CDS             complement(9235..10734)
                     /gene="pitA"
                     /locus_tag="ACHUQ7_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFR1136179.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWFSLQFL"
     gene            10966..12165
                     /locus_tag="ACHUQ7_002863"
     CDS             10966..12165
                     /locus_tag="ACHUQ7_002863"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFR1136180.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQVGRRVLLIDNGKILGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLASIVK"
BASE COUNT         3024 a         3126 c         3307 g         2885 t
ORIGIN      
        1 taacagaaat gacatattgc gctcctgatt gttgcaggta gtgtctctct tcgaagcgga
       61 taagtcaaaa acatatatga cttaacgaat gtgtaagtgc agaggaaggt aataggtgtg
      121 aattttgagt tggctattca tttgaaaggc ggtttgaatc aggacgtaaa aaaaccgact
      181 ttgcgtcggt tttttactct tcagcctgag ttggtggctc tggctggagt gagaatgccc
      241 acatctaacg gttcaatact attagccagt ggctaacatt gcaagcgtta gcgatttctt
      301 cagctggaga agggacagtt tgctatacat ggtggtgaaa gaggatttat atactttcat
      361 cgggtcgctg ccgtaccaag ccttgtccga atccggacgt aaaaaaaccg acttcgcgtc
      421 ggttttttta ctcttcagcc cagagttggt ggctctggct ggagtgagaa tgccatcatc
      481 taacgcatca atgctaatac catactgaaa ctattgcaag gttatgctgg ttatttagcc
      541 taattacact gtatgattat ccagttagct ctgaggcatt ttcactctgg caatgcgcat
      601 aaacgctttc aaagtcctgg tcagaagtac gggtggtgcc gttaactgat gctctggccg
      661 gagtgagatt gcccacatct aacaatgagg catgcgccgt gacaggcagt ggatgagtaa
      721 gcggatgcat tctcactcca tcgcatggag aaaacgggtg attgataaag caatcatcgt
      781 tcttggggcg ttaattgcgc tgctggaact gatccgcttt ctgcttcagc ttctgaactg
      841 atagcggaaa cgtaattaag ggctaagagc acactactct tagcccttta acatttaacg
      901 catggtcacg aactcttctg ccgccgttgg gtgaatggcg acggtattgt cgaagtcttt
      961 tttggttgcg cccatcttca gcgccaccgc gaagccctgc aacatttcgt ccataccaaa
     1021 gccaatgccg tgaataccga caatcttctc ttctggccca acgcacacca gcttcatgcg
     1081 gcacggctgg cggtgagtgg tgacggcggt atacatcgcg gtgaaagagg atttatacac
     1141 tttcacctga tcgtcgccgt actgctcgcg cgcctgcggt tccgttaaac caacagtacc
     1201 aatcggcgga tggctgaaga ccacggtcgg aatgttgctg taatccagat gctcatccgg
     1261 cttgttatta aacaggcgtt cagagagacg gcgacccgct gcaactgcca ccggtgtcag
     1321 ttccactgca cccgtgttat cgcccaccgc gtaaatacct tcaatattgg tgttttgata
     1381 tttatcgacg acgatatagc ctttttcgtt ggttttaacg ccagcggctt cgaggttgat
     1441 gttgtcattg gcaggctcgc gaccaatcgc ccaaatcagg caatccaccg tttcactgcg
     1501 accatcttcc agctccagcg tcaggctacc atcggcattt ttcactaccg ctttcgggat
     1561 ggcgttggta tgcaactgtg ggccttcggc gttcatcact tcgaccagcg tttcagagat
     1621 catcgggtcg aagctgcgca gcggcgcatg tttacgcaca aacagatgcg ttttcgcgcc
     1681 gaggccgtta atcacgccag ccagctcaac ggcgatgtaa cccgcgccaa caaccgccac
     1741 gcgctctggc aaagcaggaa gggcgaagaa gccatcggaa tcaataccgt attccacgcc
     1801 tggaatatcc gggtggctcg gacgaccgcc ggtggcgatc agaatatgat cggccgtgat
     1861 ggtttcgccg ttaacttcca gcgttttggc atcaacgaag cgggcaaagc ctttgattac
     1921 atcaacgtta tttttaccga gcacgttttc ataggaagta tgaatacggt cgatataggc
     1981 ggtacggctg gcgatcaacg tttcccagtt gaacttgttg atggtggtat cgaaaccata
     2041 atcagggccg tacatatgga tcgcttcacg gatttgcgcc gcgtgccaca tcactttttt
     2101 cggcacgcag ccgacgttca cgcaggtgcc gcccagctct ttggcttcaa tcagtgcgca
     2161 tttctggccg tacatagccg cgcggttgat ggaggcgata ccgccgctgc cgccgccgat
     2221 ggcgatgtaa tcatagtgtt tagtcatgac atattgtcct tatcgttgat taccgcgatt
     2281 gtagcgcgag acgtaatagg tgccagcaat ggctgcaatt actccggcac gatccagctt
     2341 acggtggcgt gcccggtacc tgccggaacc agtttgctgt gcagccacga cagcacgtta
     2401 ttcatctgct gttccagctt ccacggcggg ttaatcacaa tcatgccgga agcggtcatg
     2461 ccacggcgat cgctgtctgg cagcaccgcc agttcaattt gcagaatttt gcgaatgccg
     2521 gtcgcttcca ggtcgtggat catgcgctta atttgctggc gcagcaccac cggataccac
     2581 aatgcgtaag tgccagtggc gaaacgtttg taaccttctg ctatcccgct gaccaccgcc
     2641 tgatagtcgg ttttcatttc atacggcggg tcgataagga ttaaaccacg gcgagaaacc
     2701 ggcggcagtt tggccttaag ttgctggaaa ccgtcggctt tttcgacgcg tgcacggctg
     2761 tctttctgaa atccagaacg cagcaacggg taatcgctcg ggtgcagttc ggtcagttgc
     2821 aggctgtctt gttcgcgcag tagctggcga gcaatcaacg gcgaaccggg gtagtaacgc
     2881 aactggccgc tacggttgaa gtgttttacc acattgatgt acgcctccag ttctgcgggc
     2941 aaatcgtcct gctgccagat acgggcgata ccttcgagat attcgccggt acgctcggca
     3001 tgttcgctac ctaactgata acgcccggcc cctgcgtggg tgtcgagata gagaaacggt
     3061 ttatctttct ctttcagcga ctcgatgatc aggctctgaa cggtatgttt aaggacgtcg
     3121 gcgtggttgc cagcgtgaaa gctgtggcga taactgagca tgggtaaagg tgttccggta
     3181 agtaaaaatt ggcccgtatt cggggcgcac aaaagcctat caggacagta taccgaaaag
     3241 aggccgccgc cgcgaaagcg taacgtttct cattgaaatt cactacactt aaccccatgc
     3301 tacacacatt atgtaaagcg cctgttgagc gcttccttaa cctctttaac caggactgcg
     3361 ctaatgacga atccgttact gactcccttt gaattgcctc cgttttctaa aattctcccg
     3421 gaacatgtcg ttccagccgt gactaaggca ttgaacgact gccgcgaaaa tgtggagcgc
     3481 gtagtagcgc aaggggcacc gtacacctgg gaaaatctct gccagccgtt ggcggaagtg
     3541 gacgatgttt tggggcgtat cttctccccg gtcagccacc tgaactcggt gaaaaatagc
     3601 ccggaactgc gtgaagcgta cgaacaaacc ctgccgctgc tgtcggaata cagcacctgg
     3661 gtagggcaac atgaagggct gtataaagcg taccgcgacc tgcgcgatgg cgatcattac
     3721 gccacgctga acacggcgca gaagaaagcg gttgataacg ccctgcgtga ttttgaactc
     3781 tctggcatag gtctgccgaa agagaaacag cagcgttacg gcgaaattgc tacccgtctt
     3841 tctgaactgg gcaaccagta cagcaacaac gtcctcgatg cgacgatggg ctggaccaaa
     3901 ctcgttaccg acgaagcggg gctggcgggg atgccagaaa gcgcgctggc tgcggcaaaa
     3961 gcccaggccg aagcgaaaga gctggaaggc tacctgctga cgctggatat cccaagctac
     4021 ctgccggtaa tgacctactg cgacaaccag gctctgcgcg aagagatgta tcgcgcttac
     4081 agcacccgcg cctccgatca aggcccgaat gccggtaagt gggataacag caaggtgatg
     4141 gaagagatcc tcgcgctgcg tcacgaactg gcgcaactgc tgggctttga aaactacgcc
     4201 tttaaatccc ttgccactaa aatggcagaa aacccgcagc aggtgctgga tttcttaacc
     4261 gatctggcaa aacgcgcgcg tccgcaaggc gaaaaagagc tggagcaact gcgtgccttc
     4321 gctaaagccg aatttggtgt cgatgagttg cagccgtggg atatcgctta ctacagcgaa
     4381 aaacagaaac agcacctcta cagcatcagc gacgagcagc tgcgtccgta cttcccggaa
     4441 aacaaagcgg ttaacggcct gtttgaagtg gtgaaacgta tttacggcat caccgctaaa
     4501 gagcgtaaag atgttgatgt ctggcatccg gatgtacgtt tcttcgaact gtatgacgaa
     4561 aataacgaac tgcgcggcag cttctacctc gatctgtatg cccgtgaaaa caagcgcggc
     4621 ggggcgtgga tggatggctg cgtaggccag atgcgtaaag ctgatggttc tctgcaaaaa
     4681 ccggtcgcgt atttgacttg taacttcaac cgcccggtaa atggtaaacc ggcgctgttc
     4741 actcacgacg aagtgatcac cctgttccac gagttcggtc acggcctgca ccatatgctg
     4801 acccgcatcg aaaccgctgg tgtttccggt atcagcggtg tgccgtggga tgcggtcgaa
     4861 ctgccgagtc agtttatgga aaactggtgc tgggagccgg aggcgctggc gtttatctct
     4921 ggtcactatg aaaccggcga accgctgccg aaagagttgc tggataaaat gctggcggcg
     4981 aagaactacc aggcggcgct gtttattctg cgtcagctgg agttcggcct gtttgatttc
     5041 cgccttcatg ccgagttccg cccggatcag ggggcaaaaa tcctcgaaac tctggcagaa
     5101 atcaagaaac tggttgccgt ggtgccatct ccgtcctggg gccgtttccc gcacgctttc
     5161 agccatattt tcgccggtgg ttatgcggca ggttactaca gctacctgtg ggctgacgta
     5221 ctggcggcag atgctttctc gcgctttgag gaagagggca ttttcaaccg tgaaaccggg
     5281 cagtcgttcc tcgacaacat tctgagccgt ggcggttcag aagagccgat ggatctgttc
     5341 aaacgcttcc gtggtcgtga accgcagctg gatgcgatgc tggagcatta cggcattaag
     5401 ggctgatcat tcagtgaaaa tctgcttaat tgatgaaaca ggcgccggag acggcgcctt
     5461 atctgttctg gcggcccgct gggggctgga gcacgatgaa gacaacctga tggcgctggt
     5521 gttaacgccg gaacatctgg aattgcgcaa gcgtgatgag ccaaaacttg gcggcatctt
     5581 tgttgatttt gttggcggag cgatggcgca ccgacgcaaa ttcggcggtg gtcgcggtga
     5641 ggcggtggcg aaagcggtgg gcattaaagg cgattatttg ccggatgtgg tggacgccac
     5701 cgcagggctg gggcgcgatg cctttgtact ggcttcagtc ggctgccgcg tgcggatgct
     5761 ggagcgtaat ccagtggttg ccgcgctact cgacgacggc ctggcgcgtg gttatgcgga
     5821 tgcggaaatc ggcggctggt tgcaggagcg gttgcagtta attcacgcct ccagcctgac
     5881 ggcgctaacc gatattaccc cacgcccgca ggtggtctat ctcgatccga tgttcccgca
     5941 taagcagaaa agcgcgctgg tgaaaaaaga gatgcgtgtc tttcagtcgc tggtggggcc
     6001 ggatcttgat gccgatggat tactggagcc tgcacgcttg ctggcgacca aacgcgtggt
     6061 ggtaaagcgc ccggactacg cgccaccgct ggcgaatgtc gccacgccaa acgcggttgt
     6121 gactaaaggg catcgctttg atatctatgc aggtacgcca gtgtaaaagt aaagcagggt
     6181 agcgataacg ctaccctgct ttctgcaaga ataattaatg gctttccggc gtcgcaatca
     6241 tgcgtttcag ccacggcacc atcaacagca tcactactgc aacgcccagc gtgaccagac
     6301 caatcttacc aaacacgttg gtatagacgg gcaacgtctc aagcggatcg gtaatgttgt
     6361 ccggcaccgc ggtaaatgtt gccacatagc cgccagcaag aacgcggcag cctgcgtcag
     6421 gaaccacatc ccgagaataa agcccatcaa atgctgcggc accagggcag caatcatcgc
     6481 caggccaagg gcgctaataa acaattcacc caggctctag aataaataca ccagcacgat
     6541 aaaccatggc gatgtcagcc cctggtcatc cgcaaaccac attcccgcag cagcagccgt
     6601 caaaaagccc agtgagcaca taaacatgct gagagtaaat ttcatcggca tcgagaggtc
     6661 tttgcctttg ttacccagat gcgtgtaaat gcctgccagt attgggctgg cgagtaccac
     6721 ccagaacggg ttaagcgcct ggaagctgac cgggttgatg gaaaaaccga gaatttcatg
     6781 atgcacgttg ttgatggcaa agaagttcag cgatgttggc atctgggcgt agagaatgta
     6841 aaacaccacc gcttcgagca tcaggacaaa ggcgacaaac attttattgc gcccggtttt
     6901 atccagcttg aatgcctgac gaaagaagat gatagtgacg acgatggaga gaacaatcag
     6961 caccagattg gcgacttcta cgttgtgcat cagccatgca catacgaaga tcatcaccac
     7021 gctgccaagt aatacgtaca acagtttgct gaagctcatc ggcttgaagt cgggttcaga
     7081 accaatgtct ttcaccattc cacgacaggc gatgtaaacc agtaatgcga taattaaccc
     7141 cgccccgcac aggttgtagg tgactgaata accgaatcta tcagcgatca caggggccag
     7201 cgataacgct atcaacgagc cgatattgat cgacatatag aacagggtga atgcgccatc
     7261 aagccgcgga tctttcggcg gatagcactt cgaaagcaag ctggctgggt tagctttaaa
     7321 caggccgtta ccgacagcga tagtccccag ggcgatgaaa atcaggtcag gcttaagtag
     7381 cgacatgccg gtcatgaagt agccaatcgc cagcacaagt gctccaagaa caatggtgcg
     7441 tttggtcccc aacaggtggt cgccgacata gccgccaatg gaaatgaggc catagaccag
     7501 tgcagcaaaa gcaccaaaag tgacaaaagc ctgctcttgc gagaatccaa gctgtttaac
     7561 gaagaaaacc gccagtacgc cctgcacgcc gtagtagccg aatcgctccc ataactcgac
     7621 aaaaaagatc atgaaaaatg ggcgaggttg ctgcagcatc cccatgggtg ttgttgtatt
     7681 aatatttatt aaccttccaa taccatcctg aaaagtatga acgcgtaagg gataacgcgc
     7741 gaaaaagttt acatctgtca gagattctta atattgatgc ggtgaataga ttaatacact
     7801 attacaacag aaaataacca gctgatcaca ctaatgatgg aaaatatgat ggtgttaaat
     7861 tttgtattgg caggagattt tgattgcgta cgcagatcgt aggccagata aggggtttac
     7921 gctgcatcag gtaacaaggc gcgccaaaaa gcaaaacgcc cgcatatgcg ggcgttcagg
     7981 aacacgtcgt ggagggatga ttattcttct tcgtcgcgta gcggaacaat cagcatatca
     8041 acgtgaacgg tgttgatcag ctgacgtgcg gaagacatca gtttgctcca gaagtcctgg
     8101 tggtgaccac aaaccaccag atccatatcg tatttcttga ttgcatcgac cagaacctgg
     8161 cctaggtcgc cgctgccgct cagggtttca gtgattggat agcctgcatt agtggaaagc
     8221 tcggtcagcg catgatgtgt ctcttcagag atgcgtttct gcatatcacc cagattcaca
     8281 tcaataagcc cggtgtatag gtcagagtag tttacatcta cgtggatcag agaaactttc
     8341 gcattgtagg ggcgagccat agagactgct ttctctacca gaactttgct ttccggggag
     8401 agatcgactg cgatgagaat gtgtttataa gccatagtgt tactccttcc ataaagttgt
     8461 cgatgactgg ccagctagcg tttcttgtgc cgttacgtca cccgcgtcct gcgaactacg
     8521 tgcgcggggc taacccagcc gtaaagattc agtgttaaga ccatccttac cttatagcga
     8581 cccggatgat ccgtcaatcc gccttgctta cccgttagat aaacaaattt tcgacatgaa
     8641 tattatattg atagtggtta accttctgga aaaaaaacaa cctgatctcc tacactatct
     8701 atagagccgc tcggatgttg gtcaccacaa aagcggtttc tccaggacct ggtgttaact
     8761 gattggcagc gtatcgggag cggtagcccc ggggtggaac tccgcgggca ggtcgccggg
     8821 gaggaggtat gataagcacc gtcgcattat tttgggcttt atgtgtcgtt tgcattgtta
     8881 acatggcgcg ctatttctca tcactacgcg cgttgttagt ggtactgcgt aactgcgatc
     8941 cattgctcta tcaatatgtt gatggagggg gcttttttac ctcacatggc caacccaaca
     9001 aacaggtgcg tctcgtttgg tatatctatg cccaacgtta tcgcgatcat cacgatgatg
     9061 agtttattcg ccgctgtgag cgggtgcgtc ggcagtttat tctgactagc gcattgtgtg
     9121 gtctggtggt ggtcagcctg attgcattga tgatttggca ttaagcactt cagcaataat
     9181 aacgtataaa aaaagcgggc cagtaaactg acccgctcgt tttggtgcgt acgattacag
     9241 gaactgcaag gagaaccagt acagcccgcc ggaaagtagt accgcagccg gaagggtaaa
     9301 cacccaggcc atcagaatac tggttacggt tttacgctgt aagccgccgc catctaccac
     9361 catcgtcccc gcgacagaag aggagagtac gtgagtggtg gaaaccggca tcccggtata
     9421 actcgccagg ccgattgaca ctgccgccgt catctgggca gacatcccct gagcgtaggt
     9481 catgcctttc ttaccgattt tctcaccgat agtcgttgcc acacgacgcc agccaatcat
     9541 cgtaccgata cctaacgcca gcgcgaccgc catgatgatc cacaccggcg catactcgat
     9601 ggtgctaagc atgtcggact tcagtttctt caacaggcgc tgatcgtcag cactcacacc
     9661 aggcatcttc accactttgt cgatagtgtc agaaacgcac agcataatgc ggcgcatctg
     9721 gctacgttga tcaagcgaca gcttgtcgta gctttccaca tcggtggtca acataccttt
     9781 caggcggttg agcgcgttaa tggtattcga cggatggcag tggaactccg caggttgcgt
     9841 tgcgccagct tccggagccg gtactaactg atcagcaccg gtagcctgtt tgagcagcgc
     9901 aggatgctgc tcaaagtaag cttcgacgtt gttgatggca tcacgggtac gggtgatttc
     9961 gtagccagtg gcattcatgt tcaccacgaa gcctgctggc gcgacgccaa tcaataccaa
    10021 cataaccaga ccaatgcctt tctgaccatc gttcgcgccg tgcgaaaacg ccacgccgat
    10081 agcggaaagg atcagcgcga tacgcgtcca gaacggcggc tttttcttgc cgtctttctt
    10141 ttcacgctcc gctggggtca ggtggatacg ggcgcgtttc ttggtgccgc tccagtagcg
    10201 acgcagcaag aaaatcagac cgccagcaaa caccaggccg acaatagggg aaacgatcag
    10261 agaaccgaaa atacttaata ctttcgggat attgagtgca tccaccactg acgtcccggt
    10321 catcaacgca ttggttaaac caatcccgat gatcgcgcca atcagcgtat gagagctgga
    10381 tgcaggtaaa ccaaagtacc aggtacccag gttccagata atcgccgcca gcaacataga
    10441 gaacaccatg gcaaggccat gagacgatcc catattaagc agcagatccg tcggcagcat
    10501 atgcacaatg gcataggcaa cactcagacc acccagcaaa acacccaaaa agttgaacac
    10561 cgccgccata accacggcga gctgagaacg catcgcgcgg gtatagataa cggttgccac
    10621 ggcgttggct gtgtcatgga aaccattgat ggcttcgtag aacagcacaa aagccagtgc
    10681 aagcaataat aacagcccgg tatgcaaatc caggccagca aacaaatgta gcataggacg
    10741 ttacgccatt ttgaggacat gaacgcggcg cattatcagt gactttcgcg gcgcgggcaa
    10801 agtgaaatat agactttttt tgatttgcct cctgtatgga tttcactcaa aaaataatta
    10861 tcttatataa ttcaggcaaa tacttccttt tagtaatatt gatgctggtg cgaccactga
    10921 ggaatcttta caattcacgc ccgttttttc taagaggagc gcaacgtgga aaggtttgat
    10981 gccattatta taggcgctgg tgcggcgggt atgttctgtt ctgcgctggc aggtcaggta
    11041 ggacgccggg ttctgctgat cgataatggt aaaatactag ggcgcaaaat ccttatgtct
    11101 ggcggtgggc gctgcaactt taccaacctt tatgtcgaac caggcgctta tctgagccag
    11161 aatccgcatt tttgtaagtc tgcactcgca cgttttaccc agtgggattt cattgatctg
    11221 gtcaataaac acggtatcgc ctggcacgag aaaacgttag ggcaactctt ctgcgatgac
    11281 tccgcgcagc agattgtcga catgctggtg gatgagtgcg agaagggcaa tgtaaccttc
    11341 agattgcgta gcgaagtgct gagtgtggcg aaggatgaaa caggcttcac gcttgatctg
    11401 aacggcatga ctgtcggttg cgaaaagctg gtcatcgcga ccggtgggct gtcaatgccg
    11461 gggctgggcg catcgccgtt tggttataag attgccgaac aatttggcct caacgtgctg
    11521 ccgacccgcg cgggtctggt gccattcact ctgcataaac cgttgctcga agagttacag
    11581 gtgctggcgg gcgtggcggt gccttccgtg attaccgctg aaaacggcac cgttttccgt
    11641 gagaacttac tcttcaccca ccgcggctta tctggaccgg cggtgttgca gatttcaagc
    11701 tactggcaac cgggtgaatt tgtcagtatc aatctgctac cggatgtgga cctcgaaacc
    11761 ttcctgaatg agcagcgtaa cgcacatccg aatcaaagcc tgaaaaacac actggcggtt
    11821 catctaccga agcggttggt tgaacgctta cagcaactcg ggcaaatccc ggatgtttcg
    11881 ctaaaacagc tcaacgtgtg tgaccaacag gcactgatta gcacattgac cgactggcgc
    11941 gtacaaccca acggcactga aggctatcgc actgccgaag tgacgctcgg cggcgtggac
    12001 accaacgaac tttcttcacg gacgatggaa gcgcgcaaag tgcctgggct gtacttcatc
    12061 ggtgaagtga tggacgttac cggctggctg gggggctata acttccagtg ggcgtggtcg
    12121 agtgcatggg cttgtgcgca ggatttggcg tcaattgtga aataaccatg acgttttccc
    12181 gtcagccgtt tgtatatgtc gttattaagt cattttggta cggttctgca tccgacattc
    12241 tggtgttaac tcgctgtttt gttagcgaat taacgctatt gccgttgtgg ttattttata
    12301 gtgtatttat tctcagagaa tatatattac tgaaataaaa aa
//
LOCUS       DAZPCS010000107        12253 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZPCS010000107 DAZPCS010000000
VERSION     DAZPCS010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12253
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.107"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..247)
                     /gene="yefM"
                     /locus_tag="ACHUQ7_002864"
     CDS             complement(<1..247)
                     /gene="yefM"
                     /locus_tag="ACHUQ7_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HFR1136181.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDII"
     misc_feature    547..670
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            717..1616
                     /gene="hisG"
                     /locus_tag="ACHUQ7_002865"
     CDS             717..1616
                     /gene="hisG"
                     /locus_tag="ACHUQ7_002865"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFR1136182.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPCLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1622..2926
                     /gene="hisD"
                     /locus_tag="ACHUQ7_002866"
     CDS             1622..2926
                     /gene="hisD"
                     /locus_tag="ACHUQ7_002866"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFR1136183.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQLGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2923..3993
                     /gene="hisC"
                     /locus_tag="ACHUQ7_002867"
     CDS             2923..3993
                     /gene="hisC"
                     /locus_tag="ACHUQ7_002867"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFR1136184.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FKLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMSERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            3993..5060
                     /gene="hisB"
                     /locus_tag="ACHUQ7_002868"
     CDS             3993..5060
                     /gene="hisB"
                     /locus_tag="ACHUQ7_002868"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFR1136185.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            5060..5650
                     /gene="hisH"
                     /locus_tag="ACHUQ7_002869"
     CDS             5060..5650
                     /gene="hisH"
                     /locus_tag="ACHUQ7_002869"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFR1136186.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5650..6387
                     /gene="hisA"
                     /locus_tag="ACHUQ7_002870"
     CDS             5650..6387
                     /gene="hisA"
                     /locus_tag="ACHUQ7_002870"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFR1136187.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            6369..7145
                     /gene="hisF"
                     /locus_tag="ACHUQ7_002871"
     CDS             6369..7145
                     /gene="hisF"
                     /locus_tag="ACHUQ7_002871"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFR1136188.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            7139..7750
                     /gene="hisIE"
                     /locus_tag="ACHUQ7_002872"
     CDS             7139..7750
                     /gene="hisIE"
                     /locus_tag="ACHUQ7_002872"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFR1136189.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWIKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(7936..8913)
                     /gene="wzzB"
                     /locus_tag="ACHUQ7_002873"
     CDS             complement(7936..8913)
                     /gene="wzzB"
                     /locus_tag="ACHUQ7_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFR1136190.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(9059..10179)
                     /gene="ugd"
                     /locus_tag="ACHUQ7_002874"
                     /pseudo
     CDS             complement(9059..10179)
                     /gene="ugd"
                     /locus_tag="ACHUQ7_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
     gene            complement(10429..11835)
                     /gene="gndA"
                     /locus_tag="ACHUQ7_002875"
     CDS             complement(10429..11835)
                     /gene="gndA"
                     /locus_tag="ACHUQ7_002875"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFR1136191.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(11944..>12253)
                     /locus_tag="ACHUQ7_002876"
     CDS             complement(11944..>12253)
                     /locus_tag="ACHUQ7_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1136192.1"
                     /translation="KASEYSELYGDNLIQLGLEDGIYFYKGMAIGDVFGLARYSDWTI
                     SNPECEVIPQDDLIEKMKSFNSSFIVISKRSYANFNPEKYPKFKVLMDTPNGILIAIK
                     "
BASE COUNT         3085 a         3114 c         3070 g         2984 t
ORIGIN      
        1 caataatgtc cttttccgtt cctttgcctg atttcaggct atcgattgag tccatcaatc
       61 tccgggcgtt agcgggggaa cgcagtagat aagccgtctc ttccagcgaa ttgtattctt
      121 cgagtgacat cagaacacaa gcctctccat tctgacgagt aatgaggatc ggggcatgat
      181 cttcaacggc tttcatcatt gttgccgaca aattctgacg cgcttcgctg tagctaattg
      241 tacgcatgta aatctcctat tttgtacagt tcattgtaca atgataggtg ttaattaact
      301 atttattaat tagtttgtag atcaagacat tgccagcgag acgaaaaatc agactttgct
      361 ctttttggtg ctgtcaagtt agaggacagt cctcttagcc ccctcctttc cccgctcatt
      421 cattaaacaa atccatcgcc ataaaatata taaaaaagcc cttgctttct aacgtgaaag
      481 tggtttaggt taaaagacat cagttgaata aacattcaca gagattttta tgacacgcgt
      541 tcaatttaaa caccaccatc atcctgacta gtctttcagg cgatgtgtgc tggaagacat
      601 tcagatcttc cagtggtgca tgaacgcatg agaaagcccc cggaagatca ccttccgggg
      661 gcttttttat tgcgcggttg ttaacggttc agacaggttt aaagaggaat aagaaaatga
      721 cagacaacac tcgtttacgc atagctatgc agaaatccgg ccgtttaagt gatgactcac
      781 gcgaattact ggcgcgctgt ggcattaaaa tcaatcttca cacccagcgc ctgatcgcga
      841 tggcagaaaa catgccgatt gatattctgc gcgtgcgtga cgatgacatt ccctgtctgg
      901 taatggacgg cgtggtagac cttgggatta tcggcgaaaa cgtgctggaa gaagagctgc
      961 ttaaccgccg cgcccagggt gaagatccgc gctactttac cctgcgtcgt ctggatttcg
     1021 gcggctgccg cctttcgctg gcaacgccgg ttgatgaagc ctgggacggt ccgctctcct
     1081 taaacggtaa acgtatcgcc acctcttatc ctcacctgct caagcgttat ctcgaccaga
     1141 aaggtatctc ttttaaatcc tgcttactga acggttctgt cgaagttgcg ccgcgtgccg
     1201 ggctggcgga tgcgatttgc gatctggttt ccaccggtgc cacgctggaa gctaacggcc
     1261 tgcgcgaagt cgaagttatc taccgctcga aagcctgcct gatccagcgc gatggcgaaa
     1321 tggaagaatc taaacagcaa ctgatcgaca agctactgac ccgtattcag ggtgtgatcc
     1381 aggcgcgcga atcaaaatac atcatgatgc acgcaccgac cgaacgcctg gatgaagtca
     1441 tcgccctgct gccaggtgcc gaacgcccaa ccattctgcc gctggcgggt gatcaacagc
     1501 gcgtagcgat gcacatggtg agtagcgaaa ccctgttctg ggaaacgatg gaaaaactga
     1561 aagcactggg tgccagttca atcctggtcc tgccgattga gaagatgatg gagtgatcgc
     1621 catgagcttt aacacaatca ttgactggaa tagctgtact gcggagcaac aacgccagct
     1681 gttaatgcgc ccggcgatct ccgcttctga aagcattacc cgcaccgtta acgatattct
     1741 cgataacgtg aaaacgcgtg gcgatgaggc cctgcgtgaa tacagcgcga agtttgataa
     1801 aaccacggtt accgcgctga aggtgtctgc tgatgagatc gccgccgcca gcgaacgcct
     1861 gagcgacgag ctaaaacagg cgatggcggt ggcagtaaag agtattgaaa ccttccacac
     1921 tgcgcaaaaa ctgccgccgg tagatgtaga aacgcagcta ggcgtgcgtt gccagcaggt
     1981 cacgcgcccg gtggcttcag tgggtttgta tattccgggc ggctccgccc cgctcttctc
     2041 aacggtatta atgctggcaa ctccggcgcg tattgcgggc tgtaaaaaag tggtgctgtg
     2101 ctcaccgccg ccgattgccg atgaaattct ttatgcggcg cagctgtgcg atgtgcagga
     2161 cgtgtttaac gtcggcggcg cacaggccat tgccgcgctg gcgtttggta cggaatctgt
     2221 gccgaaagtg gacaaaatct tcgggccggg taacgccttt gtcaccgaag caaaacgcca
     2281 ggtaagccag cgtctggacg gtgcggcgat cgatatgccc gcaggcccgt cggaagtgct
     2341 ggtgattgct gacagcggcg ctacgccgga ttttgtggct tctgatttgc tttctcaggc
     2401 tgaacacggc cctgactcac aggtaatttt actgaccccc gatgctgata tggcgcgtcg
     2461 cgttgccgag gccgtcgaac gccaactggc agaactgccg cgtgccgaaa ccacccgcca
     2521 ggcactgaac gccagccgcc tgatcgtgac taaagattta gcgcagtgcg tggagatctc
     2581 caaccagtac ggcccggagc acctgatcat tcagacccgc aacgcccgcg aactggtcga
     2641 tggcatcacc agcgccggtt cggtatttct tggtgactgg tcaccggaat cggcaggcga
     2701 ctacgcctcc ggcaccaacc acgtgctgcc gacttacggt tacaccgcca cctgttccag
     2761 cctcgggctg gcagatttcc agaaacgcat gaccgtacag gaactgtcga aagaaggttt
     2821 ctccgcgctg gcttcgacca ttgaaacact ggccgccgcc gagcgcctga ccgcccacaa
     2881 aaatgccgtt actttgcgtg ttaacgtcct taaggagcaa gcatgagcac cgtgactatt
     2941 actgatttag cgcgtgaaaa cgtccgcaac ctgacaccgt atcagtcggc gcgtcgtctg
     3001 ggcggtaacg gcgacgtctg gctgaacgcc aacgaatatc ccacagccgt ggagtttaag
     3061 cttactcagc aaacgctcaa ccgctacccg gaatgccagc caaaagcggt gattgaaaat
     3121 tacgcgcaat atgcaggcgt aaaaccggaa caggtgctgg tcagccgtgg cgcggacgaa
     3181 ggtattgaac tgctgatccg cgctttttgc gaaccgggta aagacgccat cctctactgc
     3241 ccgccaacgt acggcatgta cagcgtcagc gctgaaacca ttggtgtcga atgccgcaca
     3301 gtgccgacgc tggaaaactg gcaactggac ttacagggca tttccgacaa gctggacggc
     3361 gtaaaagtgg tctatgtttg cagccccaac aaccccaccg ggcaactgat caatccgcag
     3421 gattttcgca ccctgctgga gctaacccgc ggtaaggcga ttgtggttgc cgatgaagct
     3481 tatatcgagt tttgcccgca ggcatcgtta gctggctggc tggcggaata tccgcacctg
     3541 gcgattttgc gtaccctttc gaaagccttc gcgttggcgg ggcttcgttg cggatttacg
     3601 ctggcaaacg aagaagtcat caacctgctg atgaaagtga tcccccccta cccgctctcg
     3661 acgccggttg ccgacattgc cgcacaggcg ttaagcccgc agggaatcgt cgccatgagc
     3721 gaacgggtgg cgcaaattat tacagaacgc gaatacctga ttgccgcact gaaagagatc
     3781 ccctgcgtgg agcaggtttt cgactctgaa accaactaca ttctggcgcg ctttaaagcc
     3841 tccagtgcag tgtttaaatc tttgtgggat cagggcatta tcttacgtga tcagaataaa
     3901 caaccctctt taagcggctg cctgcgaatt accgtcggaa cccgtgaaga aagccagcgc
     3961 gtcattgacg ccttacgtgc ggagcaagtt tgatgagtca gaagtatctt tttatcgatc
     4021 gcgatggaac cctgattagc gaaccgccga gtgattttca ggtggaccgt tttgacaaac
     4081 tcgcctttga accgggcgtg atcccggaat tgctgaagct gcaaaaagcg ggctacaagc
     4141 tggtgatgat cactaatcag gatggtctgg gaacacaaag tttcccgcaa gcggattttg
     4201 atggcccgca caacctgatg atgcagatct tcacctcgca aggcgtgcag tttgatgaag
     4261 tgctgatttg tccgcacctg cccgccgatg agtgcgactg ccgtaagccg aaagtaaaac
     4321 tggtggaggg ttatctggct gagcaagcga tggatcgtgc caacagttat gtaattggcg
     4381 atcgcgcgac cgacattcaa ctggcggaaa acatgggcat taatggttta cgctacgacc
     4441 gcgaaaccct gaactggcca atgattggcg agcaactcac taaacgagac cgttacgccc
     4501 atgtagtgcg caacaccaaa gagacgcaga ttgacgtcca ggtgtggctg gatcgcgaag
     4561 gtggcagcaa gattaacacc ggcgttggct tctttgatca catgctggat cagatcgcca
     4621 cccacggcgg tttccgcatg gaaatcaatg tcaaaggcga cctctatatc gacgatcacc
     4681 acaccgtcga agataccggc ctggcgctgg gcgaagcgtt aaaaattgcc ctcggcgaca
     4741 aacgcggtat ttgccgcttt ggttttgtgc tgccgatgga cgaatgcctt gcccgctgcg
     4801 cactggatat ctctggtcgc ccgcacctgg aatataaagc cgagtttacc taccagcgtg
     4861 tgggcgatct cagcactgaa atgatcgaac acttcttccg ttcgctctct tacaccatgg
     4921 gcgtgacact acacctgaaa accaaaggta aaaacgatca tcaccgtgta gaaagcctgt
     4981 tcaaagcctt tggtcgtacc ctgcgccagg ccatccgcgt cgaaggcgac accctgccct
     5041 cgtcgaaagg agtgctgtaa tgaacgtggt gatccttgat actggctgcg ccaacctgaa
     5101 ctctgtgaag tctgccattg cgcgccacgg ttatgaaccc aaagtcagcc gtgacccgga
     5161 cattgtgttg ctggccgata aactgttttt acccggcgtc ggcaccgcac aagcagcgat
     5221 ggatcaggtg cgtgagcgcg agctgtttga tctcatcaaa gcctgtaccc aaccggtgct
     5281 gggcatctgc ttagggatgc aactcctggg acgacgcagc gaagagagca atggcgtcga
     5341 cttgctgggc atcatcgacg aagacgtgcc gaaaatgacc gactttggtc tgccactgcc
     5401 acatatgggc tggaaccgcg tttatccgca ggcaggcaac cgcctgtttc aggggattga
     5461 agacggcgcg tacttttact ttgttcacag ctacgcaatg ccagtcaatc cgtggaccat
     5521 cgcccagtgt aattacggcg aaccgttcac cgcggcggta caaaaagata acttctacgg
     5581 cgtgcagttc cacccggagc gttctggtgc cgctggcgct aagttgctga aaaacttcct
     5641 ggagatgtga tgattattcc ggcattagat ttaatcgacg gcaccgtggt gcgtctccat
     5701 cagggcgatt acggcaaaca acgcgattac ggtaacgacc cgctgccgcg cttacaggat
     5761 tacgctgcgc aaggtgccga agtgttgcac ctggtggatc tgaccggggc aaaagatccg
     5821 gctaagcgtc aaatcccgct gattaaaacc ctggtcgcgg gcgttaacgt cccggtgcag
     5881 gttggcggcg gtgttcgtag tgaagaagac gtggcggcgt tactggaagc tggcgttgcg
     5941 cgcgtggtgg tcggttccac cgcagtgaaa tcaccggacg tagtgaaagg ctggtttgaa
     6001 cgtttcggtg ccgatgcgct ggtgctggca ctggatgtcc gtattgacga gcaaggcaac
     6061 aagcaagtgg cagtcagcgg ctggcaagag aactcgggcg tttcactgga acaactggtg
     6121 gaaacctatc tgcccgtcgg cctgaaacat gtgttgtgta ccgatatctc gcgcgacggc
     6181 acgctggcgg gatctaacgt ctctttatat gaagaagtgt gtgccagata tccgcaagtg
     6241 gcgtttcagt cctccggcgg cattggcgac attaacgatg ttgctgccct acgtggcact
     6301 ggcgtgcgcg gcgtaatagt tggtcgtgca ttactggaag gtaaattcac cgtgaaggag
     6361 gccatcgcat gctggcaaaa cgcataatcc cgtgtctcga cgttcgtgat ggtcaggtgg
     6421 tgaaaggcgt acagtttcgc aaccatgaaa tcattggcga tatcgtgcca ctggcaaaac
     6481 gctacgccga agaaggtgca gatgaactgg tgttctacga tatcaccgct tccagcgatg
     6541 gccgtgtggt agataaaagc tgggtatctc gcgtggcgga agtgatcgac attccgtttt
     6601 gtgtggcggg tgggattaag tctctggaag atgccgcgaa aattctttcc tttggcgcgg
     6661 ataaaatttc catcaactcc cctgcgctgg cagacccaac attaattact cgcctggccg
     6721 atcgctttgg cgtgcagtgt attgtggttg gtattgatac ctggtacgac gccgaaaccg
     6781 gtaaatatca tgtaaatcaa tataccggcg atgaaagccg cacccgcgtc actcaatggg
     6841 aaacgctcga ctgggtagag gaggtgcaaa aacgcggcgc cggagaaatc gtcctcaaca
     6901 tgatgaatca ggacggcgtg tgtaacggtt acgacctcaa gcagctgaaa aaagtgcgtg
     6961 aagtttgcca cgtcccgctg attgcctccg gtggcgcggg caccatggaa cacttcctgg
     7021 aagccttccg cgatgccgac gttgacggtg cgctggcagc ttccgtattc cacaaacaaa
     7081 taatcaatat tggcgaatta aaagcgtacc tggcaacaca gggcgtggag atcaggatat
     7141 gttaacagaa caacaacgtc gcgaactgga ctgggaaaaa accgacggtc tgatgccggt
     7201 gattgtgcaa cacgcggtat ctggcgaagt gttaatgctg ggctacatga acccggaagc
     7261 cttagacaaa accatcgaaa gcggtaaagt caccttcttc tcgcgcacta aacagcgact
     7321 gtggatcaaa ggcgaaacct cgggcaattt tctcaatgtg gtaagtattg ccccggactg
     7381 cgacaacgac acattactgg tgctggcgaa tcccatcggg ccaacctgcc acaaaggcac
     7441 cagtagctgc ttcggcaaca ccgctcacca gtggctgttc ctgtatcaac tggaacaact
     7501 gctcgccgaa cgtaaatatg ccgacccgga aacctcctac accgccaaac tgtatgccag
     7561 cggcactaaa cgcattgcgc agaaagtggg cgaagaaggc gtggaaaccg cactggcagc
     7621 aacggtacat gatcgctttg agctgaccaa cgaggcatct gacttgatgt atcacctgct
     7681 ggtgctgttg caggatcagg atctggattt aacgacagta attgagaacc tgcataaacg
     7741 gcatcagtga gttacgggct gagcaggtgt gatgttgttg tcacacccgg agccattgcc
     7801 cgatgcgcaa acttatcgtg cctacacatt atctcaacac acgtaggccg gataagatgc
     7861 gccagcatcg catccggcaa aaaaaacggg catggtgtca ccaccctgcc cgtttctctt
     7921 aaatgcacaa taatattact tcgcgttgta attacgcaga gcgttacgcc ccaacacaat
     7981 ccccgcgcca accatgccgc ccagaagcac tgccagaatc aaggtgattg cctttttcgg
     8041 actatcgcga cgaataggta acgtcggttt catcacatag cggtaagcat gaatatcaag
     8101 atcatcaaac tttaattttt caatatccaa caggttttga cgtgtctgat agtagtttgg
     8161 tgagaacacc aacggacgag tcgcttcatg cttaatcatc gactccagcg cttcgctccc
     8221 tagaaggaac aacgtatctt gcgtcacatc ttcagtctgc tgaacctgtg gctttgtcac
     8281 ctgcgcctga ttcgcatact gcaacgcttc ctgaatctga cggatacgca gatctttctg
     8341 ctcctgcgcg accacttcct gggttcttaa agagtcctgc aagtttttcc gtcccagagc
     8401 aatgttgtcc ttgagatcct tttctagctc ttgattcact ttatcatcaa cttgctgaat
     8461 gtattgggcc aacttcattt gtgcgccctc tgcagtttgc ccaacataag aaacagtcaa
     8521 tggtaattgc tggttcttaa cagaaggttc gatggtaagt ttttctggct cttcctgatt
     8581 atccagcgtt tctgctaatg cagagaaggc agaactgaag cgaccaatta acgtctcctg
     8641 caaatccgat actttcggtg cagcctgacc atagataaca ttcatggcat tgttatagcc
     8701 agcaatttgc cccacgtcgg gctgagtgat aattgctgtt gacgtccatt tctccttcgc
     8761 tactgccaaa taaccaatag ccagggcaat agccacaatg acggaaatga taattgtcat
     8821 cttgccacgc cacaactgca ctagtaaatc aatcaaatca atctgttccg ggtcatggtt
     8881 ttgcccagaa acattattat tttctactct catcattacc ctaactgacg gaaatttcca
     8941 agtccgcata gtttagcgat aatccccgtg aatgctatag gataagtgat aaaaataatg
     9001 aattattgcg gaggtaacaa aagacaatca gggcgtaaat agccctgata acaagatgtt
     9061 agtcgctgcc aaagagatcg cgggtgtaga ccttatctgc cacatcctta agctcttctg
     9121 ccatacggtt ggagataatg acgtcggctt gttgtttgaa ggtggcgaga tcacgttcca
     9181 ggcgagagtt gaagaatgag tcttctttca tcaccggctc gtagatgatc acttcaacgc
     9241 ctttcgcctt gatacgcttc ataatcccct gaatagaaga cgcacggaag ttatctgacc
     9301 cgctcttcat aatcagacga taaataccca ccacttgcgg tttgcatcga caattgccga
     9361 gatcaggtta ttcggcacag actggtagtt cgccagtaac tgcttggtat ctttcggcag
     9421 acaataacca ccataaccaa acgacggatt gttgtaatgg ttgccaatac gcggatcgag
     9481 acaaacgcct tcgataatct ggcgagtatt cagacctaaa ctttctgcat aactatccag
     9541 ttcattaaag tatgctacgc gcatcgccag ataggtatta gcgaaaagtt taatcgcttc
     9601 tgcttcagtg gagtcggtaa acagggtcgg gatattttgc ttaatcgcgc cttcctgtaa
     9661 caacgcagca aaacgttctg cgcgttctga acgctcaccg atgacaatac gtgacggatg
     9721 gagattatcg taaagggctt taccttcgcg gaggaattcc ggagagaaaa taatattttc
     9781 agtacgatat ttcttatgca tcgctgcggt aaaaccaacg ggaaccgttg atttgatgac
     9841 cataaccgca taaggattta tctcaactac gtctttaatt actgattcta cactggatgt
     9901 attgaaataa ttagttttag gatcatagtc ggttggagtg gcgatgatga cataatcagc
     9961 atcccggtag gcttcatttt tatctaatgt ggcattaaag tgtattttat ctgattgcaa
    10021 aaactgctga atttccttat caacaatagg agatatccga tcattcagca tagcaacgcg
    10081 tgacggtaaa atatctaatg ccacaacctc atgattttgt gcgattagaa gcccgtttga
    10141 caagcctaca tagccagtac cggaaatggt gattttcatt tcatgctctc agaattaact
    10201 taactgtgaa tcatgatgtt tttagcatcg tgataagatg ctaaaagttt taacgctacg
    10261 ttgtcagatg gtcaacgcaa cgaaaaacaa ttcagatttt ctttataaga atagctgatg
    10321 tttattaagt taatattaag cagtgaaatt tagtctataa gatatttggc aaaaaaaagc
    10381 ccggtgcaat atacgccggg cctcaatttt attgttggtt aaatcagatt aatccagcca
    10441 ttcagtatgg aacacacctt ctttatcaat gcgcttataa gtatgcgccc cgaaatagtc
    10501 acgctgtgcc tggatcaggt tcgcaggcag aaccgcggca cggtagctat cgtaataggc
    10561 aaccgcagcg gcgaaggtcg gaaccgggat accgttctgt actgcataag cgacgacatc
    10621 gcgcagcgcc tgctggtagt catcggcaat ttgcttgaag tacggggcca gcagcaggtt
    10681 agcgatctgc ggattttcgg cataagcatc ggtgattttc tgcaggaact gcgcacggat
    10741 gatgcagcca gcacggaaaa tcttcgcgat ttcgccgtag ttcagatccc aattgtattc
    10801 ttcagacgca gcacgcagct gagagaagcc ctgagcgtaa gaaacgattt tgcccagata
    10861 cagcgcactg cgaacttttt cgatgaactc agccttgtcg cctgctgact gagcttgcgg
    10921 accagagaga actttagatg cggcaacgcg ctgatctttc agagaagaga tataacgtgc
    10981 aaacacagac tcggtaatca gcgacagcgg ttcgccgaga tccagcgcgc tctggctggt
    11041 ccatttaccg gtgcctttgt ttgctgcttc atccagaatc acatcaacca ggtagttacc
    11101 gtcttcatct tttttggtga agatatcttt agtgatgtcg atcaggtagc tgctaagttc
    11161 accgttattc cactcggtga aagtctgggc cagttcttca ttagatagat tcaggccacc
    11221 tttgagcaga gaatacgctt cagcaatgag ctgcatatct ccgtattcaa taccattgtg
    11281 aaccatcttc acataatggc ctgcgccgtc ggcaccaata taagtaacgc atggctcgcc
    11341 gtcttctgcc acggcagcga ttttagtcag gataggcgca accagctcat aagcctcttt
    11401 ctgcccgcca ggcataatag aaggaccttt cagcgcgccc tcttcaccgc cggaaacacc
    11461 ggtaccgatg aagttaaagc cctctgctga aagctcacga ttacgacgaa tggtgtcctg
    11521 gaagaaggta ttaccaccat caatgatgat gtcaccttta tcgagatatg gtttcaggga
    11581 atcaatcgca gcatccgtgc ctgcacctgc tttcaccatt aacaggatgc gacgaggcgt
    11641 ttccagagat tcaacaaact ctttcaccgt atagtaagga gccagtttct tgcctggatt
    11701 ttcagcaatc acttcttccg tcttttcgcg ggagcggttg aaaatagaga cggtataacc
    11761 acgactttcg atgttgagcg caaggttgcg ccccatcact gccataccga ctacgccgat
    11821 ctgttgcttt gacattgttt actcctgtct tctatattat taaccgtcta ttaaccgtta
    11881 tattattaac cgtcgtgatt ttcatcttct aatcaaggaa tacatatggt tacagcatgc
    11941 ttcttacttt atagcgataa gtatgccatt gggtgtatcc ataagaactt tgaattttgg
    12001 atacttttct ggattaaaat tagcatagct tcgtttagat ataacaataa acgaactatt
    12061 aaaagatttc atcttctcta ttaaatcatc ctgtgggatt acctcacact cgggattact
    12121 aatagtccaa tcactatatc ttgctaatcc aaagacgtca ccaattgcca tccctttata
    12181 gaagtaaata ccatcctcca agcctaattg gataagatta tctccatata actcactata
    12241 ttcacttgct ttc
//
LOCUS       DAZPCS010000108        12153 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZPCS010000108 DAZPCS010000000
VERSION     DAZPCS010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12153
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.108"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            126..995
                     /locus_tag="ACHUQ7_002877"
     CDS             126..995
                     /locus_tag="ACHUQ7_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFR1136193.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1198..1551)
                     /locus_tag="ACHUQ7_002878"
     CDS             complement(1198..1551)
                     /locus_tag="ACHUQ7_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFR1136194.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1689..3335)
                     /gene="groL"
                     /locus_tag="ACHUQ7_002879"
     CDS             complement(1689..3335)
                     /gene="groL"
                     /locus_tag="ACHUQ7_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFR1136195.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3379..3672)
                     /locus_tag="ACHUQ7_002880"
     CDS             complement(3379..3672)
                     /locus_tag="ACHUQ7_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFR1136196.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3948..5204
                     /gene="yjeH"
                     /locus_tag="ACHUQ7_002881"
     CDS             3948..5204
                     /gene="yjeH"
                     /locus_tag="ACHUQ7_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFR1136197.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(5220..5696)
                     /locus_tag="ACHUQ7_002882"
     CDS             complement(5220..5696)
                     /locus_tag="ACHUQ7_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFR1136198.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            6033..7469
                     /gene="aspA"
                     /locus_tag="ACHUQ7_002883"
     CDS             6033..7469
                     /gene="aspA"
                     /locus_tag="ACHUQ7_002883"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFR1136199.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7587..8888
                     /gene="dcuA"
                     /locus_tag="ACHUQ7_002884"
     CDS             7587..8888
                     /gene="dcuA"
                     /locus_tag="ACHUQ7_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFR1136200.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            9004..9342
                     /gene="cutA"
                     /locus_tag="ACHUQ7_002885"
     CDS             9004..9342
                     /gene="cutA"
                     /locus_tag="ACHUQ7_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFR1136201.1"
                     /translation="MLDEKSSNTTSVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9318..11015
                     /gene="dsbD"
                     /locus_tag="ACHUQ7_002886"
     CDS             9318..11015
                     /gene="dsbD"
                     /locus_tag="ACHUQ7_002886"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFR1136202.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADEQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPSGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWPGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            11052..11627
                     /locus_tag="ACHUQ7_002887"
     CDS             11052..11627
                     /locus_tag="ACHUQ7_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR1136203.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDKLRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11734..11809
                     /locus_tag="ACHUQ7_002888"
     tRNA            11734..11809
                     /locus_tag="ACHUQ7_002888"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11767..11769,aa:Phe,seq:gaa)
BASE COUNT         2933 a         3140 c         2979 g         3101 t
ORIGIN      
        1 ttctgtttat atagagtgag cacggctatt tatgttattt taacaactat tactaccaag
       61 cccatttatc tggcttataa attaaaatta attatttaat tgagaaatgg ttagggagaa
      121 cctacatggc cataagcatc aagggtgtta ataccggtgt tattcgcaag agtaataatt
      181 tcattgcact ggcgctgaaa attaaagaac ctcgcaataa agaatcgttg ttctttatgt
      241 ctgtcatgga actgcgcgac ctgctgattg cgctggaaag tcgtctgcat caaaagcata
      301 aactggatgc tgctgcacgc cttcagtatg agcaagctcg cgataaagtc attaagaaaa
      361 tggcagaaaa catcccagaa attctggttg atgagcttaa aaatgccgat attaatcgtc
      421 gggttaacac gctggaactt accgataatc aaggcgaaaa tcttaccttc gtattaacgc
      481 tacatgacgg tagcaaatgt gagttagtcg tcaatgagtt gcagattgaa atgctggcgc
      541 gagcaatcat tcatgcaatt aataatgcgg aaatgcgtga actggcatta cgtattacct
      601 cactgttaga tttcctgccg ttatacgacg ttgactgcca ggacaatggc aatctggaat
      661 atgacaccta ttcccaaccg gaatggaaac ataacttgtt cgaccattat ctggcggtgc
      721 tttaccgctt taaagatgaa agtggcaaag aacaatttag cggcgcagtg gtgaaaacgc
      781 gtgaagcaac gccgggtaaa gaaatagagg cgatcactcg ccgaatgctt gatttcagcc
      841 cacgactgaa aaaactcgcg ggtgtgccat gtcaggtcta tgttcgcacc gtagcggcga
      901 ataacgccca accgctaacc caggatcaat gcttacgcgc gctgcatcac ttacgtgtac
      961 aatccaccag caaaactgca ccacaggcga aataatctca tctttgctaa tgcgaggctg
     1021 gagccatcca gcctgaaatg actgataacg tgatcgttgg tcatgccgga tgcggcgtaa
     1081 acgccttatc cggcctacaa attctcaagc attcaatcag ttgcaatgcc tgctgtaggc
     1141 ctgataagcg aagcgcatca ggcaataata cgtttgtcac ccagcctctg gatcacatca
     1201 gttcgggcac ttataaacct gaccgataat ctggctatcc gtcgggacaa aactggacaa
     1261 cattccctgc gacgggctgc tgatgccata aatcacgtta ccgcccattg cagccgcctg
     1321 gttgcgcaga tcgtttgctg cgccgcgcat agaaccgccc tcttctccgt gttgcccgga
     1381 aagccagttg ctttgcttac ctgtcgcagt accaatcagc tggcactctg cgcctggctg
     1441 ctcgtccaca atgcgtacac tctgaccggc agcactcaat tcgttgctgg agctacaacc
     1501 cgccatcagt agcgcggcac cgacaatccc tgctaagtat tttacgtgca tgttattccc
     1561 cataatcaat gagctggacg ccactcgcgt catccgtgtc tgaatcttat actagaaaga
     1621 tgaccaaaag aaaaaccccc agacatttct gcccgggggt ttgtttattt ctgcgaagtg
     1681 cagggcaatt acatcatgcc gcccatgcca cccatgccgc ccataccgcc agcagcgcct
     1741 aagtcagctg catcgttttt cggcaggtcg gtaaccatgc attcggtggt gatcatcagg
     1801 ccagccacag aagctgcgta ctgcagagca gaacgggtta ctttggttgg gtccaggata
     1861 cccatgtcga tcatgttgcc gtattcttcg gttgctgcgt tgtaaccgta gttgccgtcg
     1921 ccgcctttaa cggtgttagc aacaacagac ggttcttcgc cgcagttcag gacgatctga
     1981 cgcagcggag cttccattgc acgcagtgca actttgatac ccacgttctg gtcttcgttc
     2041 tgaccacgca ggtcagccag tttagacgct acgcggatca gcgcaacacc accaccagca
     2101 accacgcctt cttctaccgc agcacgggtc gcgtgcaggg catcttcaac gcgtgctttt
     2161 ttctctttca tttcaacttc ggtagcagca cctactttga taactgcaac gccgcctgcc
     2221 agtttcgcta cgcgctcctg cagtttttca cggtcgtagt cagaagttgc ttcttcaatc
     2281 tgctgacgga tctgagcaac acggccctgg attgcagctt cttcacccac gccatcgatg
     2341 atagtggtgg tgtctttgtt gatcacaaca cgtttagcct gacccaggtc ttccagggtt
     2401 gctttttcca gctccatacc gatctcttca gagattacgg taccgccagt cagggttgcg
     2461 atatcctgca gcatagcttt acgacgatcg ccgaagcccg gagctttaac cgcagcaact
     2521 ttcacgatgc cacgcatggt gttaacaacc agagttgcta gcgcttcgcc ttcaacatct
     2581 tcagcgatga tcagcagcgg tttgcctgct ttggcaacag cttccagaac cggcagcatt
     2641 tcgcggatgt tggagatttt cttgtcagcc agcaggatga acgggctttc cagttctact
     2701 gcgccagttt ccggcttgtt gatgaagtaa ggagacaggt agccacggtc gaactgcata
     2761 ccttcaacca cgtccagttc gtcctgcaga ccggtaccgt cttcaacggt gataacgcct
     2821 tctttaccga ctttgtccat cgcttcagcg atcagtttac ctacggtttc gtcggagtta
     2881 gcggagatgg taccaacctg agcaatcgct ttagaatcag agcacggtac ggacagcgct
     2941 ttcagttctt caactgcagc ggtaaccgct ttgtcgatac cacgtttcag gtccatcggg
     3001 ttcatgcccg cagcaacagc tttcagacct tcagtgatga tagcctgagc cagtacggtt
     3061 gcagtggtgg taccgtcgcc tgcagcgtcg ttcgctttgg aggcaacttc tttcaccatc
     3121 tgcgcaccca tattttcgaa cttgtcttcc agttcgattt cacgagcaac ggaaacacca
     3181 tctttggtga tggtcggtgc accgaaagat ttatccagaa ctacgttacg gccttttgga
     3241 ccgagggtaa ctttcactgc atctgccagt acgtttacgc cgcgcagcat tttcacacga
     3301 gcgtcgttac cgaattttac gtctttagct gccattatct ttattcctta aattcgtatg
     3361 ttcagtgtcg tgcgcggatt acgcttcaac aattgccaga atgtcgcttt cggacatgat
     3421 caacacttct tcattgtcga tcttctcgga tttcacaccg tagccatcgt tgaaaataac
     3481 gatgtcgcca actttcacgt ccagcggctt cacttcgcca ttttcaagga tacggccatt
     3541 gccgacagcc agcacttcgc cgcgggtgga tttagccgct gcagagccgg tcagaacgat
     3601 gccgccagca gatttagttt caacttcttt acgcttgacg atcacgcgat catgcaatgg
     3661 acgaatattc attgataact ctcctttgag aaagtccgta tctgttatgg gtgacgccgg
     3721 agcttacgtg gtttcccggc tggtgaccag agaaatgggg atgaggcttc gccccttcaa
     3781 gggggaaaaa gaaaaaaaat tctgatcagc acaaaatcgg gtgaaaaccc tgattcacct
     3841 cacatttcat cgcaatttct tcatcgccgt cataagcgaa tctgattgtg ctaccatcga
     3901 aaatctacgc agttgcccaa aatttgggcg caatcggata tcaacccatg agtggactca
     3961 aacaagaact ggggctggcc cagggcattg gcctgctatc gacgtcatta ttaggcactg
     4021 gcgtgtttgc cgttcctgcg ttagctgcac tggtagcagg caataacagc ctgtgggcgt
     4081 ggcccgtttt gattatctta gtgttcccga ttgcgattgt gtttgcgatt ctgggtcgcc
     4141 actatcccag cgcaggcggc gtcgcgcact tcgtcggtat ggcgtttggt tcgcggcttg
     4201 agcgagtcac cggctggctg tttttatcgg tcattcccgt gggtttgcct gccgcactac
     4261 aaattgccgc cggattcggc caagcaatgt ttggctggca tagctggcaa ctgttgttgg
     4321 cagaactcgg tacgctggcg ttggtgtggt atatcggtac tctcggtgcc agttccagtg
     4381 ctaatctaca aaccgttatt gccggactta tcgtcgcact gattgtcgct atctggtggg
     4441 cgggcgatat caaacctgcg agtatcccct tccccgcgcc aggaaatatc gaacttaccg
     4501 ggttatttgc tgcgttatca gtgatgttct ggtgttttgt cggtctggaa gcatttgccc
     4561 atcttgcctc ggaatttaaa aatccagagc gtgattttcc tcgtgctttg atgattggtc
     4621 tgctgctggc aggattagtc tactggggct gtacggtagt cgtcttacac ttcgacgcct
     4681 atggtgaaca aatggcggcg gcagcatcgc ttccaaaaat tgtagtacag ttgttcggtg
     4741 taggagcgtt atggattgcc tgcgtaattg gctatctggc ctgctttgcc agtcttaaca
     4801 tttatataca gagcttcgcc cgcctggtct ggtcgcaggc gcaacataat cctgaccact
     4861 acctggcacg cctctcttct cgccatatcc cgaataatgc cctcaatgcg gtgcttggct
     4921 gctgcgtggt gagcacgttg gtaattcatg ccttagagat caatctggac gctcttatta
     4981 tttatgccaa tggcatcttt attatgattt atctgttatg catgctggca ggctgtaaat
     5041 tattgcaagg tcgttatcga ctactggcgg tggttggcgg gctgttatgc gttctgttac
     5101 tggcaatggt cggctggaaa agtctctatg cgctgatcat gctggtgggg ttatggctgt
     5161 ttctgccaaa acgaaaaacg ccggaaaatg gcataaccac ataatccggc gtttcgacat
     5221 taatcctggc gatcgtcttt atgatcaagg cggtcgcggt catcatcctt tcgctgatac
     5281 tcaccatcaa aagtattacc gccaccggtc ccggcgctaa aaccgccgcc tggcatgcga
     5341 gaaaagcgca aatgtggcat caacttcact gtcagatgct tttgcaccgg cggcaataaa
     5401 agcagaagac cgaggaagtc ggtgaaaaag cccggtaata aaagcagcaa accggcaata
     5461 atcagcgaaa cacttttaat catctccgcc gcagggtttt cacccgccgc cattttttgc
     5521 tgcatcagca cgaaattctt aaagccctgg ttacgtacca gtgacatacc gataactgac
     5581 gtgaatataa ccagcacgag ggtcagcaat acccccaata catgggcaac ctgaataaag
     5641 attgaaatct caatatagac ataaaggaaa atggcaataa aaggtaacca gcgcaaaggt
     5701 ttctcctgta atagcagccg gttaaacccg gctacctgaa tgggttgcga atcgccttca
     5761 gcttatattg tggtcattag caaaatttca agatgtttgc gcaactattt ttggtagtaa
     5821 tcccaaagcg gtgatctatt tcacaaatta ataattaagg ggtaaaaacc gacacttaaa
     5881 gtgatccaga ttacggtaga aatcctcaag cagcatatga tctcgggtat tcggtcgatg
     5941 caggggataa tcgtcggtcg aaaaacattc gaaaccacat atattctgtg tgtttaaagc
     6001 aaatcattgg cagcttgaaa aagaaggttc acatgtcaaa caacattcgt atcgaagaag
     6061 atctgttggg taccagggaa gttccagctg atgcctacta tggtgttcac actctgagag
     6121 cgattgaaaa cttctatatc agcaacaaca aaatcagtga tattcctgaa tttgttcgcg
     6181 gtatggtaat ggttaaaaaa gccgcagcta tggcaaacaa agagctgcaa accattccta
     6241 aaagtgtagc gaatgccatc attgccgcat gtgatgaagt cctgaacaac ggaaaatgca
     6301 tggatcagtt cccggtagac gtctaccagg gcggcgcagg tacttccgta aacatgaaca
     6361 ccaacgaagt gctggccaat atcggtctgg aactgatggg tcaccagaaa ggtgaatatc
     6421 agtacctgaa cccgaacgac catgttaaca aatgtcagtc cactaacgac gcctacccga
     6481 ccggtttccg tatcgcagtt tactcttccc tgattaagct ggtagatgcg attaaccaac
     6541 tgcgtgaagg ctttgaacgt aaagctgtcg aattccagga catcctgaaa atgggtcgta
     6601 cccagctgca ggacgcagta ccgatgaccc tcggtcagga attccgcgct ttcagcatcc
     6661 tgctgaaaga agaagtgaaa aacatccaac gtaccgctga actgctgctg gaagttaacc
     6721 ttggcgcaac agcaatcggt actggtctga acacgccgaa agagtactct ccgctggcag
     6781 tgaaaaaact ggctgaagtc actggcttcc catgcgtacc ggctgaagac ctgatcgaag
     6841 cgacctctga ctgcggcgct tatgttatgg ttcacggcgc gctgaaacgc ctggctgtga
     6901 agatgtccaa aatctgtaac gacctgcgct tgctctcttc tggtccacgt gccggcctga
     6961 acgagatcaa cctgccggaa ctgcaggcgg gctcttccat catgccagct aaagtaaacc
     7021 cggttgttcc ggaagtggtt aaccaggtat gcttcaaagt catcggtaac gacaccactg
     7081 ttaccatggc agcagaagca ggtcagctgc agttgaacgt tatggaaccg gtcattggcc
     7141 aggccatgtt cgaatccgtt cacattctga ccaacgcttg ctacaacctg ctggaaaaat
     7201 gcattaacgg catcactgct aacaaagaag tgtgcgaagg ttacgtttac aactctatcg
     7261 gtatcgttac ttacctgaac ccgttcatcg gtcaccacaa cggtgacatc gtgggtaaaa
     7321 tctgtgccga aaccggtaag agtgtacgtg aagtcgttct ggaacgcggt ctgttgactg
     7381 aagcggaact tgacgatatt ttctccgtac agaatctgat gcacccggct tacaaagcaa
     7441 aacgctatac tgatgaaagc gaacagtaat cgtacagggt agtacaaata aaaaaggcac
     7501 gtcagatgac gtgccttttt tcttgtgagc agtaacttaa aaataacaat ctaatatcaa
     7561 cttgttaaaa aacaaggaag gctaatatgc tagttgtaga gctcatcata gttttgctgg
     7621 cgatcttctt gggcgccaga ttggggggaa taggtattgg ttttgcaggc ggattggggg
     7681 tgctggttct tgccgctatt ggcgttaaac ccggtaacat cccgttcgat gtcatttcca
     7741 ttatcatggc ggttatcgcc gctatttctg ccatgcaggt tgctggcggt ctggactatc
     7801 tggttcatca gacagaaaag ctgctgcgcc gtaacccgaa atacatcacg atcctcgcac
     7861 cgatcgtgac ctatttcctg actatctttg ctggtactgg caacatctct ctggcgacac
     7921 tgccagttat cgctgaagtt gcgaaggaac aaggcgttaa accttgccgt ccgctctcta
     7981 ctgcagtggt atccgcgcag attgcgatca ccgcatcgcc aatctcagcg gcagtggttt
     8041 acatgtcttc cgtgatggaa ggtcatggca tcagctacct ccatctgctc tccgtggtca
     8101 tcccgtccac cctgctggcg gttctggtga tgtccttcct ggtcactatg ctgttcaact
     8161 ccaaactctc tgacgatccg atttatcgca agcgtctgga agagggcttg gttgaactgc
     8221 gcggtgaaaa gcagattgaa atcaaatccg gtgcaaaaac gtccgtctgg ctgttcctgc
     8281 tgggcgtagt tggcgtggtt atctatgcaa tcatcaacag cccaagcatg ggtctggttg
     8341 aaaaaccgct gatgaacacc accaacgcaa tcctgatcat catgctcagc gttgcaactc
     8401 tgaccaccgt tatctgtaaa gtcgataccg acaacatcct caactccagc accttcaaag
     8461 caggtatgag cgcctgtatt tgtatcctgg gtgttgcgtg gctgggcgat actttcgttt
     8521 ccaacaacat cgactggatc aaagataccg ctggtgaagt gattcagggt catccgtggc
     8581 tgctggccgt catcttcttc tttgcttctg ctctgctgta ctctcaggct gcaaccgcaa
     8641 aagcactgat gccgatggct ctggcactga acgtttcacc gctgaccgct gttgcttctt
     8701 tcgctgcggt gtctggtctg ttcattctgc cgacctaccc gacgctggtt gctgcggtac
     8761 agatggatga cacgggtact acccgtatcg gtaaattcgt cttcaaccat ccgttcttca
     8821 tcccgggtac tctgggtgtt gccctggccg tttgcttcgg cttcgtgctg ggtagcttca
     8881 tgctgtaatg atcaatcgcg gggcgttcac gccccgcttt ctttcccgcc gactaacatc
     8941 cttcccccgt ccgttgtata gtgacctctc tcttgcggtt ccatctgttc ttgcgaggtg
     9001 tttatgcttg atgaaaaaag ttcgaatacc acgtctgtcg tggtgctatg taccgcaccg
     9061 gatgaagcga cagcccagga tttagccgcc aaagtgctgg cggaaaaact ggcggcctgc
     9121 gcgaccttga tccccggcgc tacctctctc tattactggg aaggtaagct ggagcaagaa
     9181 tacgaagtgc agatgatttt aaaaactacc gtatctcacc agcaggcact gctggaatgc
     9241 ctgaagtctc atcatccata tcaaaccccg gaacttctgg ttttacctgt tacacacgga
     9301 gacacagatt acctctcatg gctcaacgca tctttacgct gattctgcta ctttgcagca
     9361 cttccgtttt tgccggatta ttcgacgcgc cgggacgttc acaatttgtc cccgcggatc
     9421 aagcctttgc ttttgatttt cagcaaaacc aacatgacct taatctgacc tggcagatca
     9481 aagacggtta ctacctctac cgtaaacaga tccgcattac gccggaacac gcgaaaattg
     9541 ccgacgagca gctgccgcaa ggcgtctggc atgaagatga gttttacggc aaaagcgaga
     9601 tttaccgcga tcggctgacg cttcccgtaa ccatcaacca ggcgagtgcg ggagcgacgt
     9661 taactgtcac ctaccagggc tgtgctgatg ccggtttctg ttatccgcca gaaaccaaaa
     9721 ccgttccgtt aagcgaagtg gtcgccaaca acgcagcgtc acagcctgtg tctgtttcgc
     9781 agcaagagca gcacaccgcg caattgccct tttccgcgct ctgggcgttg ttgatcggta
     9841 ttggtatcgc ctttacgcca tgcgtgctgc caatgtaccc actgatttct ggcatcgtgc
     9901 tgggtggtaa acagcggctc tccactgcca gagcattgtt gctgaccttt atttatgtgc
     9961 aggggatggc actgacttac acggcgctgg gtctggtagt tgccgccgca gggttacagt
    10021 tccaggcggc gctacagcac ccatacgtgc tcattggcct cgccatcgtc tttaccttgc
    10081 tggcgatgtc aatgtttggc ttgtttactc tacaactccc ctcttcgctg caaacacgtc
    10141 tcacgttgat gagcaatcgc caacagggcg gctcacctag cggtgtgttt gttatggggg
    10201 cgattgccgg actgatctgt tcaccatgca ccaccgcacc gcttagcgcg attctgctgt
    10261 atatcgccca aagcgggaac atgtggccgg gcggcggcac gctttatctc tatgcgttgg
    10321 gcatgggcct gccgctgatg ctaattaccg tctttggtaa ccgcttgctg ccgaaaagcg
    10381 gcccgtggat ggaacaagtc aaaaccgcgt ttggttttgt gatcctcgca ctgccggtct
    10441 tcctgctgga gcgagtgatt ggtgatgtat ggggattacg cttgtggtcg gcgctgggtg
    10501 tcgcattctt tggctgggcc tttatcacca gcctacaggc taaacgcggc tggatgcgcg
    10561 tggtgcaaat aatcctgctg gcagcggcat tggttagcgt gcgcccactt caggattggg
    10621 catttggtgc gacgcatacc gcgcaaactc aaacgcatct caactttaca caaatcaaaa
    10681 cggtagatga gttaaatcag gcgctcgttg aagccaaagg caaaccggtg atgttagatc
    10741 tttatgccga ctggtgcgtc gcctgtaaag agtttgagaa atacaccttc agcgacccgc
    10801 aggtgcaaaa agcgttagca gacacggtct tacttcaggc caacgtcacg gccaacgacg
    10861 cacaagatgt ggcgctgtta aagcatctta atgtccttgg cctaccgaca attctctttt
    10921 ttgacggaca aggccaggag catccacaag cacgcgtcac gggctttatg gatgctgaaa
    10981 ccttcagcgc acatttgcgc gatcgccaac cgtgaacgac acttgcagtg ggaaagacgg
    11041 aggaaaatag cgtgcaacgt gaagatgtac tgggagaagc cctgaaatta ttagaattac
    11101 aagggattgc caacaccacg ctggagatgg ttgctgaacg tgtggattat ccactggaca
    11161 agctacgccg cttctggcca gacaaagagg caatcctcta cgatgcgctg cgttatctta
    11221 gccaacagat agatgtctgg cgtcgtcagc tgatgctgga cgaaacgcaa accgccgaac
    11281 aaaagctgct ggcacgttat caggcgttat cggagtgcgt taaaaacaac cgctatccgg
    11341 gctgtctatt tatcgctgcc tgtacgtttt atcccgatcc tggccaccct attcatcaac
    11401 tggccgatca gcaaaaaagc gctgcctacg atttcaccca cgaactgtta accacgctgg
    11461 aagttgacga tccggcgatg gtagcaaagc agatggaact ggtgctggaa ggctgtttaa
    11521 gccgaatgct ggtgaatcgt agccaggcgg atgtcgacac tgcacatcgg ctggcggaag
    11581 atatcctgcg cttcgcccgc tgccgccagg gtggtgcatt gacctgacag aaacacagaa
    11641 aagaagcgat ttgccgcaat cttaagcagt tgaatcgctt ttactgaaat taggttgacg
    11701 agatgtgcag attacggttt aatgcgcccc gttgcccgga tagctcagtc ggtagagcag
    11761 gggattgaaa atccccgtgt ccttggttcg attccgagtc cgggcaccaa attcatatca
    11821 acggacctcc acggaggtcc gtttttcgtt tcaggacgcc acgatttaag cgttctgcct
    11881 ccaaatcaat tctaccgaac tcaaccagat tctccccaca tcaccagcaa tttgcgagca
    11941 tatcccaatt tgggaaaatt tgttcctgag ctatagcact aactgacgtg aaatgtcgtg
    12001 cggccccgtg atgctgttga acgtcaaatg acgtcatcag gagcgtaacg tatccataaa
    12061 gcacaacacc ggtctgaacg ccgactgatg agattttctg aatgagaaca aagagaaatg
    12121 tgtcagtccg cttgctcatg caaagactaa caa
//
LOCUS       DAZPCS010000109        12114 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZPCS010000109 DAZPCS010000000
VERSION     DAZPCS010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12114
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.109"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..371
                     /locus_tag="ACHUQ7_002889"
     CDS             <1..371
                     /locus_tag="ACHUQ7_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1136204.1"
                     /translation="TTLMSWDAVLYDLRSEKIISKDNYLTDLNRRHLELVLHTNPNPS
                     GSVTKILRTIFTKQVKSLGPELCHFLHHELTKKRYEVWHHYDVLHYQSSSFSRSQFNA
                     LINLLAQQSRFADLKLSNYK"
     gene            complement(459..1103)
                     /gene="cheZ"
                     /locus_tag="ACHUQ7_002890"
     CDS             complement(459..1103)
                     /gene="cheZ"
                     /locus_tag="ACHUQ7_002890"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFR1136205.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTHMLRDSLRELGLVQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(1114..1503)
                     /gene="cheY"
                     /locus_tag="ACHUQ7_002891"
     CDS             complement(1114..1503)
                     /gene="cheY"
                     /locus_tag="ACHUQ7_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFR1136206.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1518..2567)
                     /gene="cheB"
                     /locus_tag="ACHUQ7_002892"
     CDS             complement(1518..2567)
                     /gene="cheB"
                     /locus_tag="ACHUQ7_002892"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFR1136207.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLATISAGQAIRI"
     gene            complement(2570..3430)
                     /gene="cheR"
                     /locus_tag="ACHUQ7_002893"
     CDS             complement(2570..3430)
                     /gene="cheR"
                     /locus_tag="ACHUQ7_002893"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFR1136208.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEDRVWSAAASTGEEPYSIAMTLADTLGTVPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3449..5050)
                     /gene="tap"
                     /locus_tag="ACHUQ7_002894"
     CDS             complement(3449..5050)
                     /gene="tap"
                     /locus_tag="ACHUQ7_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFR1136209.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNHGE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSHEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNSSLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(5096..6757)
                     /gene="tar"
                     /locus_tag="ACHUQ7_002895"
     CDS             complement(5096..6757)
                     /gene="tar"
                     /locus_tag="ACHUQ7_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFR1136210.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNFDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHICEIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSALAAKEIKALIEDSVSRIDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRITEQDPNWETF"
     gene            complement(6902..7405)
                     /gene="cheW"
                     /locus_tag="ACHUQ7_002896"
     CDS             complement(6902..7405)
                     /gene="cheW"
                     /locus_tag="ACHUQ7_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFR1136211.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(7426..9390)
                     /gene="cheA"
                     /locus_tag="ACHUQ7_002897"
     CDS             complement(7426..9390)
                     /gene="cheA"
                     /locus_tag="ACHUQ7_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFR1136212.1"
                     /translation="MSIDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASYDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKAGEVDLLEEELGHLTTLNDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKIATPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(9395..10321)
                     /gene="motB"
                     /locus_tag="ACHUQ7_002898"
     CDS             complement(9395..10321)
                     /gene="motB"
                     /locus_tag="ACHUQ7_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFR1136213.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRLHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(10318..11205)
                     /gene="motA"
                     /locus_tag="ACHUQ7_002899"
     CDS             complement(10318..11205)
                     /gene="motA"
                     /locus_tag="ACHUQ7_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFR1136214.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(11332..11910)
                     /gene="flhC"
                     /locus_tag="ACHUQ7_002900"
     CDS             complement(11332..11910)
                     /gene="flhC"
                     /locus_tag="ACHUQ7_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFR1136215.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(11913..>12114)
                     /locus_tag="ACHUQ7_002901"
     CDS             complement(11913..>12114)
                     /locus_tag="ACHUQ7_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFR1136216.1"
                     /translation="LPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGI
                     MLSTRLLNDVNQPEEALRKKRA"
BASE COUNT         2785 a         3388 c         2933 g         3008 t
ORIGIN      
        1 acactacgct aatgagttgg gatgccgtgc tctatgatct acgtagtgaa aaaattatca
       61 gtaaagataa ttacttgacc gacttaaacc gcagacatct tgaacttgtt cttcatacta
      121 accccaatcc aagcggttcg gtaacgaaga ttttacgcac tatttttact aagcaggtaa
      181 agagtttagg gccggaactc tgtcatttct tgcaccatga actgacgaaa aaacgttatg
      241 aagtatggca tcactacgat gtactgcact accagtcatc gtcattttct cgttcacaat
      301 ttaatgcatt gataaattta ctggcgcaac aatcacgctt cgctgatcta aaattatcaa
      361 actacaaata accatcctcc acgtactccc tgaacgatac ccgccaccct gctatcggct
      421 tggcatcatc gcgcaggccg aagttcaggc aatacaaatc aaaatccaag actatccaac
      481 aaatcgtcca cctgatcctg actggctacc acgccggctt tgctggtatc gacctgaggt
      541 ccattaagca aactctggtt ttcacgtttt ggacgcgact cctgttccgg gatgttttcc
      601 aacagcacca tcagcaactg gcgttcgatc tcctgaatga catccatcat ccgcttaata
      661 acctgcccgg tgagatcctg aaaatcctgc gccatcatga tttccagcag ttgcgcgtta
      721 gtaaagctgg tatgcgcggg tacatctgcc agaaattgtc gtgtatctgt taccagttca
      781 cgggcgtcgg caaggtcaat cggatcggca aaccagtcat cccaacgttg ggttaacgct
      841 tttgctgatt tctccatttg atcctgatgc ggttgtgacg cctcaacact gttcagcgcc
      901 cgctccgcag cctgggcggt catctgcaca acatagtaca aacgatcgcg cgcatcgggg
      961 atggcttccg ccgcttcggc aatggcctga accagcccca gttcccgcaa actgtcgcgc
     1021 agcatatgcg tcaggctgcc gatgcgcgca atgatatcgc cagctgaatg ctcgtcagca
     1081 ggtttgattg atggttgcat catagtcgca tcctcacatg cccagtttct caaagatttt
     1141 gttgagtttt tcctccagcg tcgcggcggt aaatggcttc accacatagc cactggcccc
     1201 cgcttgcgcc gcagcaatga tgttctcttt cttcgcttct gcagtcacca ttaacactgg
     1261 caatgccgac atcgcgccat ccgcacgaat tgttttcagc aattccaggc catccatatt
     1321 gggcatgttc cagtcggaga taacaaatcc ataaccgcct gcctgcaact tattgagagc
     1381 gtcgacacca tcttccgctt cctcaacatt attgaatccc agctctttca gcaggttacg
     1441 cactatgcgt cgcatggtgg aaaagtcatc cacaaccaaa aatttaagtt ctttatccgc
     1501 catttcacac tcctgattta aatacgtatc gcctgtccgg cactaattgt tgccaacatt
     1561 tgctggctta cctggctaag atcgaccact tcgcagacac cacccatatt gatggcctcg
     1621 cgcggcatgc cgaacaccac gcaacttgct tcgttttgcg caagggtcca tgcccccgcc
     1681 tgacgcatcg ccaacattcc cgccgcgccg tcgttgccca taccggtcag gatcacccca
     1741 accgcattac gccccgcctg tttggcgaca gaatggaaca acacatctac cgaaggccga
     1801 tgacggttaa ccgccgggcc gtcgtgaatt ttgatttgat aatttgcgcc actacgcgcc
     1861 agttccatat gccgatcgcc cggcgcaata taggcatgcc ccggcaagac acgttctccg
     1921 tcttcggctt ctttaacccc gatctggcaa agcttattaa gtctgtcggc aaaagagcgg
     1981 gtgaaaccgg gcggcatatg ttgggtaatt aacaatgccg ggctggaaag cggcaacggt
     2041 tgcagtacgt gacgaattgc ctcagttcca cccgttgaag caccaatcgc aatcagtttt
     2101 tcagaactca acaacggccc tgccttcagc gttgtcggtg ctgacaatgg cttatgtgct
     2161 gcaaggctcg cctttgctgc cgtacgcacc ttttcagcaa tcatttcgct atacgccagc
     2221 attccttcgc gaatacccag ttgcggtttg gtgacaaaat ctattgcccc cagctccagc
     2281 gcgcgcagcg tgacttctga tcctttgccg gtcaaggaag aaaccatcac aacgggcatt
     2341 ggacgcaaac gcattaattt ttcgaggaaa tccagtccgt ccatccgcgg catttcaaca
     2401 tccagcgtca gcacatcggg attgaatttc ttaatcaagt cacgcgcaac cagcggatca
     2461 ggcgcggtcg ccaccatttc catgtcgcta tggctgttga tgatttctgt catgatctgg
     2521 cgcatcagtg ccgaatcatc gacagataac accctgattt tgctcatcgt taatccttac
     2581 ttagcgcata caccgtctga ccacgcagcg tgaagcggcg ctcgaggtgg ctaaagtttt
     2641 cagagtgacc cgcaaacagc aatccgtcgg gtttaaggag cggaacaaag cggcgcaaaa
     2701 tctcctgctg ggtagtttga tcgaagtaga tcatgacgtt acgacagaag atcgcatcaa
     2761 acggccccgg cacggtgtac tgtttcgcca gtaaattcag cggggcaaaa tcaacatagt
     2821 tcgccagctc ctgacgcacg cgtaccagcc cttcatgcgg tcccgtccct cgcatgaaat
     2881 accgttgcag ttgctgcggc gtcaggtttt tcaactcttc atggcgatag ataccgcttc
     2941 tggctttttc cagcacttcg gtgtcgatat cactggcaaa cactttccag cgtccgggca
     3001 cggtgcccaa tgtgtcagcc agagtcatcg caatgctgta cggctcttcg ccggtcgaag
     3061 ccgccgcgct ccatacgcga tcctcgccag aacggcgacg tgcgtgatcc gcgagcagag
     3121 ggaaatgatg agcctcacgg aaaaatgccg tcagattcgt ggtcagcgaa ttgataaacg
     3181 cctgccactc accgctgtgc tgattagatt ccagcaagtt cagataatga ccgaaatccg
     3241 tcagtcccag agaacgcaaa cgacgaacca gtcggttgta aaccatgtcg cgtttatggt
     3301 cagccagaac gatcccggct cgttgataga tcaattgact tatccgccga aaatgcgcgt
     3361 cggaaagcgc caggcgctcg gtcatctgta acaataaaga cgtttgccca cagggcagag
     3421 atgaagtcat agcgccttct caatcacttc aggataccac tggcgcaatt tgtaactgcg
     3481 ccgactcatg tcgtgctact tcatgttctt caagggtaaa taccgccaca cgcgacgaaa
     3541 gatggtcggc ctgattcgcc agttgttccg ttgccaccgc cgcttcttct accagcgagg
     3601 agttctgctg agtcacctga tccatctggc tgacagcctg tgcaacctgc tcaatccccc
     3661 gccgttgttc ttccgacgcc gaagcaattt ctcccataat gtcgttcacg cgggtcaccg
     3721 aactgacaat atcggtcatg gtcgcggcgg cgttattcac cagtttcgaa ccctgctgga
     3781 cacgattgac tgactcttcg atcagccctt tgatctcttt tgctgcctgc gcgctacggc
     3841 tggcaagatt gcgcacttca cctgccacta ccgcaaaacc acgcccctgc tctccggcgc
     3901 gagccgcttc cactgccgca ttcagggcca gaatattggt ctggaaagca attccgtcga
     3961 taacgctgat aatgtcgcca attttttgcg agctggtggc gatctcctgc atggtgtgag
     4021 tcatggtact gacctgaaca ccgcccgcct gcgccgttgt cgcggcattt tttgccagtt
     4081 ccgacgcctg tcgtgcgtta tcggcgtttt gccctaccgt ggcggttaat tgctccatac
     4141 tggcggccgt ttgtgccagc gatgccgcct gctgttcggt acgacttgag agatcgttat
     4201 tgcctgcgac aatctccgcg atcccaatgt gcatctcatg gcttccctta cgcacatcac
     4261 tcaccgtccc acgcaaagcc tgctgcatgg tcttcaggct ggcaaaaatg gcggtgatct
     4321 cattacgacc atataccgca atcggacgcg ccagattacc cgcagcaatg ctgtcaaaat
     4381 ggctaccgat aatggccagt ggttgaacaa tcatcttgcg cgtccaccac agcgcactgc
     4441 tgatgtagat cgccgcaaca ataatcatgc tgataaacac cagcgccgaa atctgatagt
     4501 tacgctggct ttgcgcactg gcggcttcca gcacatggtt gatctccagc tgccaggcct
     4561 caaagttaac gtcaaacgca ttctgtgatc cctgcaccgg cgcagtgagg aaatccgaaa
     4621 gttgattact ttcaagccag gtcgcctgat gttcgagatc gttgtgccag cgggcaaaac
     4681 ttttctccgt ctctttttgc aatgccctga cgtgctcgtt gcccgcagtc atcgccataa
     4741 aacttttaaa cagcgtggtg gattgcgtca gactggcgcg cgccgtcgtc atcaacgttt
     4801 taatgtcatc cgccggatag ctaagcgctg tcagagtgcc tgctttgttc agcgcggtac
     4861 tggcctgtaa cattaccgcc cgagtttgcg ctaatgccgc acgttgctga ttgctctgct
     4921 cgccgtgatt cagtcgttgc aaatcgtcgc gaaatgccca aaaagacatg ccgttactgc
     4981 caatctgcaa gatcccgcag agaatcaaaa ttaaaaacag cgtggtcgaa attcgaatac
     5041 gattaaacat caacgctccc catcaggcgg caatgaccgc gttagtaaaa actcgtcaaa
     5101 atgtttccca gtttggatct tgttcagtaa ttcgcagtcg tggctgtgcc ggtggttgct
     5161 cactggcagg acgggatggt gtttgcggtt tattggtgag tgggctggct gccagacgga
     5221 acgcggaaac cgcttgcgtt aaacgactcg cctgttcttc cagcgcagct gcggcggcag
     5281 ctgattcctg caccagcgat gcgttctgtt gcgtgacgcg atccatttcc gaaaccgcca
     5341 atgcgacttg atcgatgcca cggctctgtt catccgatgc cgatgcaatc tcgcccataa
     5401 tgtcagtcac gcgagtgaca gcattgacga tattgttcat tgtttccccg gcgctttcga
     5461 ccagcaccga accggtatca atgcgcgaga cggagtcttc aatgagggct ttgatctctt
     5521 ttgccgccag ggcgctgcga ctggcaagat tacgcacttc acccgccacc acggcaaaac
     5581 cacggccctg ttcacccgca cgcgcggctt caaccgcggc attcagcgcg aggatattag
     5641 tctggaaggc aataccgtcg ataacgctga taatgtcggc aattttcttc gaactatcgg
     5701 cgatctcatg catcgttttc actacgccat ccaccacttt gccgccgtgc tgggcggtgt
     5761 cggaggcact ttgcgccagt tgcgaggcct ggcgggcgtt atcggcgttt tgcttcactg
     5821 tcgcggtgag ctgctccatg ctggcggcag tttcttccag cgcggatgcc tgctgttcag
     5881 tacgggagga aagatcggtg ttgcccgccg caatttcacg ggtaccggca tagatggcat
     5941 ctgaaccttc gcggacatga gtgacggtgt cagtcaaaga gcgttgcata tgtgaaacgc
     6001 tctgcgccag gtcacccatt tcactgcgcc cgtcaatggt cagggtattc gccaggttac
     6061 caccggcgat ttcgcaaatg tgagcaataa tttttgccag cggagtaagc aacatacggc
     6121 gaatgccgta ccacgccacc agcagaatca ataccaccac cagcgcgata accgccagtt
     6181 gccactgggc aaatcggtaa tcatctgcgt tgtcagtgac gatatcgcga tacagttttt
     6241 cactgctgag ggcgtactga gcaaacgctt cgcccattgc attttgcatt ccctgggttg
     6301 gctgagcgaa ataagctcca gtattgccat aatcaagata atcaatcagt tcagttaacg
     6361 ctgtgtaata gtttttatat ttttcatcaa aattacggct ggtagcgacc atttcaggta
     6421 acggtgccat gcttttgaat tttttataat gcgtcgcggc ctgggccaat gttttcctgg
     6481 cgctatcgag caattcaact ttggcgttac tttgttgatt ggaggaatcc atcatcatcc
     6541 gcaccgctga acgactcagg ttaatgcgcg tttgcagcat taaatcccag gttgacgtca
     6601 gctcgccctg ctgttcccgt aattgattgg aaaccacaaa gctcttctgg ctatggtgaa
     6661 gggaagaaaa aaacaaactg ccggaaataa gctgtaacag tgcgaatacc cccagcacca
     6721 ttaccaacag cgtgactacg cggatacggt taatcataag gcaccttcct gaaaacaagt
     6781 tgatctcgtt atcggcaaga aggggggaaa ctttattgct gatgccaccc gccgcgaaat
     6841 tgaaataaaa aacccgatgc gcagatcatc gggttcattt caattgagga aatcgggaga
     6901 attacgccac ttctgacgcc gcgctatcta acagcgccat ctcttcgctg ttcagcagtt
     6961 tttcgatgtt caccagaatc aacatacggt cgcccagtgc gcccagtcca gtgagatatt
     7021 ctgttgaaag cgtcacggca aactctggtg ccggacgaat ttgctccgcc gtcaatgaaa
     7081 gcacgtctga aacgccgtca accacgatgc cgaccacccg ctgtccgaga ttcaggacga
     7141 taactaccgt gttgtcgtta tagtccacat ccacctggct gaacttaatt cgtaagtcaa
     7201 caatcggcac aataacgccg cgcagattcg tgacgccttt gataaacgct ggcgtgttcg
     7261 caatccgtgt tacctgatcg tagccacgga tctcctgcac tttcaggata tcaataccgt
     7321 actcttcatc accaagggta aataccagaa attcctggcc tgacggctcg ctggccagct
     7381 ttgttacatt cgtcataccg gtcatattgt taccttttta ctcattcagg cggcggtgtt
     7441 cgccatacgt tgttcgcggt ttatcgcctg caaggcggag acatcaacaa tcagtgccac
     7501 gctgccgtcg ccaagaatgg tcgcagcaga aatgccgggg actttgcgat agttactttc
     7561 aaggttttta accacaacct ggtgttgacc aattaattga tccaccagca aggcgtagcg
     7621 gcgaccgcca ctttgtaaga tcaccacaat tccctgggtg gcttcggttt tcgcgcccgc
     7681 gacgttgaac actttccaca gttcgacgat gggcagatat tcaccccgca cttccagcac
     7741 ccgctcgccg ccggccagtg gatggagatc ggcttcacgg ggttgcagtg attccataac
     7801 agcattcagc ggcagaatga aaacttcatc cgcaacgcgt acggacatgc cgtcgaggat
     7861 ggccagcgtc agcggcagta aaatgcggat cgtagtgcca gtaccctgct tcgactggat
     7921 ttcgacatga ccgcccatct cctggatatt acgtttaacg acgtccatgc cgacgccgcg
     7981 cccggagacg tcggtgacct gctctgccgt ggagaagcct ggtgcaaata tcagcattgc
     8041 aacttcgtcg tcgctcatgt tttcgctgac agtcaaacct tgcgaggccg cttttgccag
     8101 aattcgctca cggtttagcc ccgccccatc gtcggtcact tcaatgcaaa tgttgccgcc
     8161 ctgatgttcg gcagacagaa ttaaatttcc gacgctgttt ttacctgcgg cgagccgttt
     8221 ttctggcagt tcaataccgt gatcgaggct attgcgtacc aggtgggtca gcgggtcgat
     8281 aatgcgttct atcaggctct tgtcgagctc ggtggaactg cccaccagcg tcagttctac
     8341 ctgcttgccg agttttcccg ccagatcacg caccagccgg ggatagcgac taaagacata
     8401 ttccatcggc atcatgcgaa tcgacatcac tgattcctgc aaatcacggg cgttacgttg
     8461 taactgcccc atgctggtaa tcaaatcacc atgattaacc gggtccagtt cgctggaacg
     8521 ctgggcaagc atggactggg tgataaccag ctcgccgacg aggttaatta attgatcaac
     8581 cttttctacc gctacacgga tgctggtgga ttcactgctg cgcgtcgttt tttcccgctc
     8641 cacgcggccg gttggcgctt gttcggctgc cagtttaagc acaggtgggg tagctatttt
     8701 tggcgaaact tctactgttt caaaggtaat ctgatcggct tcaatcacaa aacagagtac
     8761 cgctgtgatg tcatcttcgg cgatatcgcc cggtaatatt gccgagagag aatccgcccc
     8821 tttcaccacg tcatttaacg ttgtcagatg ccccagctct tcttccagca ggtcgacttc
     8881 gccggccttc aggcgcgaaa ggataattcg tcgcggcaac tgactgcgac tttgctcatc
     8941 ttgcggttca cttttggcaa ccacacttaa tcgggtcact gcggatggcg tttcgccttt
     9001 cgcttctaat gccagttgac gcaaggcctg gcagatataa tcgtagctgg cggcatccgg
     9061 ctcttgcgac tgtttataag cgtcgagctg ttcttgcatg atatccttcg tttccaaaaa
     9121 cagattgata atgtcggtgt tgagttgcat ctcacctcgt ctggcttcat cgagcaggtt
     9181 ttccatcaga tgcgtggttt cctgcaaaac gctgaagcca aaagttcctg cccctccttt
     9241 gatcgagtgg gcagcccgaa agatggcatt caattgttcg gcatctggcg cttccggctg
     9301 caaaaccagc agatgctgct ccatatcagc caacagttcg tccgcttcat caaaaaatgt
     9361 ctgataaaaa tcgcttatat cgatgctcac gctgtcacct cggttcggct gatggcattg
     9421 tgggaacact gacctgtggt gcaacctcag gtttttctag ggcgcttact ggctcattct
     9481 ggctttcggc gttttcatgc aaaatggcct gttcggcttg tttgttcagt accaacaggc
     9541 tgatgcgacg gttgacggca tcatcaggtc cgcgatcgct taagcgcatc gttgccgcca
     9601 tgccgacgac acgtaacact ttgccgctat ccaaccctcc gaccatcagt tcgcggcggg
     9661 atgcattggc ccgatcggca gaaagctccc agttgctgta acctttctca ccgctggcgt
     9721 agggaaaatc atcggtatga cctgaaaggc taatacggtt gggaataccg ttcagcacag
     9781 gcgcgatggc gcgcagaatg tcgcgcatat agggttcgac atcggcactg ccggttctaa
     9841 acatcgggcg attctggcta tcgatgatct gaatacgtag accttcctgg accagatcga
     9901 ttttgagatg gagacgtaac gcccgcagtt tcggatcgga ctctatcaac tggtcgagat
     9961 caccccgcaa tttccgcaat cgactttgct ccatgcgttt tttcagctct tcgatgttcg
    10021 gctgcttatt cacttccccc tggctttggg tgtaatcatc accaccgccg ggaattgggc
    10081 tttcactatt ggaaatgcga tcgccgcccg taaccgctgt cgccagtgga gtccggaagt
    10141 actccgcaat ctgaatcaac tcttttgggc tggagatgga gatcagccac atcaccagaa
    10201 aaaaggccat catcgcagtc ataaagtcgg cataagcaat cttccacgat ccatgtgctg
    10261 ccccgtggct tttggctttg cgtcgtttga cgacaataat cggatgcgct tgattcttca
    10321 tgcttcctcg gttgtcgtct gttgttgcgg atttttcacc gcacgcacat gctcttccag
    10381 ttcaataaac gacggacgtt cgctggaata gagcgttttg cgaccaaact caacggcgat
    10441 aggcggtgcg taaccgttca gattagaaag cagagtgact ttgacgcact gcatcatttt
    10501 gctggtttcg gcgcttttct gacgtaaaac actcgctagt ggggaaataa atccgtaagc
    10561 caataaaata ccgaggaaag tccccaccat cgcatgtgcg ataagcgccc ccagctcggc
    10621 ggcaggacga tcggctgaac ctaacgcgtg aacgcccccc attacagccg cgacaatacc
    10681 aaacgccgga agtgagtccc cgaccagcgc cagactgttc gccgggactt ctgcctcgct
    10741 ttcgtgcgtc tcaatctctt catccatcag ggcttcgatt tcgaaggtgt tcatgtgacc
    10801 gctgataatc aggcgcagat aatcgacgat aaaatcaagc atgacgctat ccgcgaggat
    10861 gcgtgggtag ctggcgaaga tctcgctctc acggggattt tcaatatcac gttccagcga
    10921 aaacatcccc atctgccgcg atttcgccat caaccgataa agcagagcca gcagatccat
    10981 atacattgct ttggtgtatt tggagcgacg aaacagcaac ggcagcgctt tcagcgtgcc
    11041 tttaatcgct ttgccattat tgccgacgat aaacgaccca atccctgcac cggcaataat
    11101 caccagttca gcgggttgat agagtgctcc aaggcttcca ccggtcatca aataaccgcc
    11161 gaaaactgta ccgagaacaa ccaggtaacc taataagata agcacgacat catccttcca
    11221 ctgttgacca tgacaggatg ttcagtcgtc aggcgttaac gcgcgattgg ggaaaaaaaa
    11281 agcagcggta cgtcgttacc gctgctggaa tgttgcgcca caccgtatca gttaaacagc
    11341 ctgtactctc tgttcatcca gcagttgtgg gataatatcg gcaggattct gggaaagttt
    11401 acgtcttttt actgcccggg atggcggttg acataagctg caggcaaagc tgccaacagg
    11461 ctggtgagcg tgggtaataa aattgccgcc gcagcagttg cagctggaaa gttgcagtaa
    11521 tccactttca acaaaccgca ccaatgtcca ggcacgggtt aatgccagca gtggtccttc
    11581 ttctgcttgt gggcactgtt caaggtataa acggtaggct ttgatcaccg catcgacgcc
    11641 attacacaaa ccggttttca gtaaaaactg ccatgcatta cagaacatcg aagcatgaac
    11701 gttttgttcc caggtcataa accagtcggt tgagaatggc agcatgcctt tcggcggtgg
    11761 gcttccgcgc agttctttat aaagttttat caggcgtccg cgacttaact gtgtttcgct
    11821 ttccagcatc tgcaaacgag cgcccagggt gatcaattcc attgccagct gaatatcccg
    11881 cgcttcctga acaatgcttt tttcactcat gatcaggccc ttttcttgcg cagcgcttct
    11941 tcaggctgat taacatcatt cagcaagcgt gttgagagca tgatgccggt atgaatttgc
    12001 tggagatcgt caacgcggga atcttgcgtc aactgagtaa tcgtctggtg gctgtcaaaa
    12061 cggaagtgac aaaccagctg attggtttct gccagcttaa ccatttgcgg aaga
//
LOCUS       DAZPCS010000110        12001 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZPCS010000110 DAZPCS010000000
VERSION     DAZPCS010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12001
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.110"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            224..994
                     /locus_tag="ACHUQ7_002902"
     CDS             224..994
                     /locus_tag="ACHUQ7_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFR1136217.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            985..1458
                     /locus_tag="ACHUQ7_002903"
     CDS             985..1458
                     /locus_tag="ACHUQ7_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFR1136218.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISSVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1565..2104
                     /gene="dnaT"
                     /locus_tag="ACHUQ7_002904"
     CDS             1565..2104
                     /gene="dnaT"
                     /locus_tag="ACHUQ7_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFR1136219.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2107..2844
                     /gene="dnaC"
                     /locus_tag="ACHUQ7_002905"
     CDS             2107..2844
                     /gene="dnaC"
                     /locus_tag="ACHUQ7_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFR1136220.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2893..3387
                     /gene="yjjA"
                     /locus_tag="ACHUQ7_002906"
     CDS             2893..3387
                     /gene="yjjA"
                     /locus_tag="ACHUQ7_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFR1136221.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3627..5918
                     /gene="opgB"
                     /locus_tag="ACHUQ7_002907"
     CDS             3627..5918
                     /gene="opgB"
                     /locus_tag="ACHUQ7_002907"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFR1136222.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6056..7078)
                     /gene="lgoD"
                     /locus_tag="ACHUQ7_002908"
                     /pseudo
     CDS             complement(6056..7078)
                     /gene="lgoD"
                     /locus_tag="ACHUQ7_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
     gene            7217..8131
                     /locus_tag="ACHUQ7_002909"
     CDS             7217..8131
                     /locus_tag="ACHUQ7_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFR1136223.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSQLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPRETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8346..9707
                     /locus_tag="ACHUQ7_002910"
     CDS             8346..9707
                     /locus_tag="ACHUQ7_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1136224.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(9756..11420)
                     /gene="tsr"
                     /locus_tag="ACHUQ7_002911"
     CDS             complement(9756..11420)
                     /gene="tsr"
                     /locus_tag="ACHUQ7_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFR1136225.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQPTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAVREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11539..11985)
                     /gene="hpaR"
                     /locus_tag="ACHUQ7_002912"
     CDS             complement(11539..11985)
                     /gene="hpaR"
                     /locus_tag="ACHUQ7_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HFR1136226.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
BASE COUNT         2919 a         3172 c         2919 g         2991 t
ORIGIN      
        1 cccgtaaagg ctaaacaata aattcattat agtgataata ttcatgtgat agtttgtttg
       61 cggcgagaga taattcgctt tttatcaccg agcttgtccc aataaccagg attaatcagc
      121 gcaattttga tcttaaccta ccgcaaaaca accgtgcttc atgatacaaa tggattctca
      181 ttatctgaac aatacgcagc acgtctatga caaagggcga gttatgcaaa ctgagcaaca
      241 gcgagccgta acgcggcttt gtatccagtg tggattattt cttttgcaac atggtgcgga
      301 aagcgcgttg gttgatgagc tttcctcacg actgggtcgg gcgctgggaa tggacagcgt
      361 cgaaagttct atctcttcga acgccatagt gctgacaact attaaagatg ggcaatgcct
      421 gacatcgaca cgtaaaaatc acgatcgcgg cattaatatg catgtggtga ctgaagtcca
      481 gcacattgtg attcttgcgg agcatcatct gctggattac aaaggcgtag agaaacgatt
      541 tagccaaatt caaccattac gttacccaag atggctggta gccttaatgg ttggcctttc
      601 ttgcgcctgt ttctgtaaac tcaataacgg tggctgggat ggtgccgtca tcaccttctt
      661 tgccagtacg accgcgatgt atatccgcca gctgctggca caacgtcatc ttcatccaca
      721 gatcaacttt tgccttaccg ctttcgccgc caccaccatt tccggattgc ttttgcaact
      781 ccccactttc agcaataccc ccaccattgc gatggccgcc agcgttctgc tgctagtgcc
      841 gggctttccg ttgattaatg ccgtcgccga tatgtttaaa ggccacatca ataccggact
      901 ggcacgctgg gcgatcgcca gtctgctgac actggctacc tgcgtcggcg tagtgatggc
      961 actgacgatt tgggggctac gcggatgggt gtgatcgaat ttctgttagc gttggcgcag
     1021 gatatgatcc tcgccgccat tcctgcggtc ggctttgcga tggtgttcaa cgttcccgtg
     1081 cgggcgttac gctggtgtgc gctgcttggc tcgataggtc atggttcccg aatgatcttg
     1141 atgaccagcg ggttgaatat tgagtggtca acctttatgg cttctatgct ggtcggtacc
     1201 attggtattc aatggtcgcg ctggtatctg gcgcatccga aagtgtttac cgtggcggcc
     1261 gttatcccta tgttcccggg catatcggct tataccgcaa tgatttcgtc ggtaaaaatc
     1321 agccagttag gttacagcga accgttgatg attaccctgt taaccaactt tcttacagct
     1381 tcatcgattg ttggtgcgtt atccatcggt ctttccattc ctggattatg gttgtaccgc
     1441 aagcgccctc gcgtataaaa ttgcctcgct taagcacacg gatgagagac agcctcctct
     1501 cctccgtgtg ttactataaa agttatctcc cttctcgttc atcgttccat atttgagaaa
     1561 cagtatgtct tccagagttt tgaccccgga cgtcgttggt attgacgccc tggtacacga
     1621 tcaccaaacc gttctggcaa aagctgaagg cggtgtggtt gccgtatttg ctaacaatgc
     1681 cccggcgttt tatgccgtca cgcctgcacg cctggctgaa ctgctggcgc tggaagaaaa
     1741 gctagcgcgt ccgggaagcg atgtcgctct ggacgatcaa ctctatcagg aaccgcaagc
     1801 cgctcccgtt gctgtaccca tggggaaatt cgccatgtat ccggactggc aacccgatgc
     1861 cgattttatc cgcctggcgg cgctatgggg cgtggcgcta agagagccgg tgaccaccga
     1921 agaactggcc tcattcattg cctactggca ggcggaaggt aaagtctttc accatgtgca
     1981 gtggcaacaa aaactggcgc gcagcctgca aatcggtcgt gccagcaacg gcggactgcc
     2041 gaaacgagat gtgaatacgg tcagtgaacc tgacagccaa attccaccag gattcagagg
     2101 gtaacgatga aaaacgttgg cgacctgatg caacgcctgc aaaaaatgat gcctgcccat
     2161 atcaaacctg cattcaaaac gggtgaagag ctactggcct ggcagaaaga acaaggggcg
     2221 atccgctccg ccgctctcga acgtgaaaat cgggcgatga aaatgcagcg cacctttaac
     2281 cgctccggta ttcgtccact gcatcagaac tgctcctttg agaactatcg cgttgagtgt
     2341 gaagggcaga tgaatgcgtt aagcaaagcg cgccagtatg tcgaagagtt tgacggcaac
     2401 atcgccagct tcatcttttc tggtaagcca ggaaccggca aaaaccatct ggcggcggca
     2461 atctgcaacg agctgctgct acgcggtaaa tccgtattga tcatcaccgt ggccgatatt
     2521 atgtcggcga tgaaagatac cttcaggaat agcggtacca gcgaagaaca actgcttaac
     2581 gatctgagca acgttgatct gctggtgatc gatgagatcg gcgtgcagac cgaatcgaaa
     2641 tacgaaaaag tgatcatcaa ccagatcgtc gatcgccgct cttcttccaa acgcccaacc
     2701 gggatgctga ccaacagtaa tatggaagag atgaccaagt tgctgggcga acgcgtgatg
     2761 gaccgtatgc gcctgggtaa cagtttatgg gtgatcttca actgggatag ctaccgtagc
     2821 cgggtaacgg gtaaagagta ttaatccccg tttctggcgg tatactgcga cacaattcca
     2881 ccttgagtaa tgatgatgaa aactattaaa catcttctgt gctgtgccat tgccgccagc
     2941 gcattaattt ccaccggggt gcatgctgcg tcctggaaag atgcgctctc cagcgccgcc
     3001 agcgaacttg gcaaccaaaa cagcacgaca caggaaggcg gttggtcgct cgcgtcatta
     3061 actaacttgc ttagcagcgg aaaccaagcc ttaagcgcag ataacatgaa caatgccgca
     3121 ggcattctgc aatactgcgc gaagcaaaag ctggcttcgg taaccgatgc cgaaaacatc
     3181 aagaaccagg tgctggaaaa gctgggcctg aacagtgaag agcaaaaaga agacaccaac
     3241 tatctggacg gtattcaggg tttgctgaaa acaaaagatg gtcagcaact caatctggat
     3301 aacatcggaa cgactccgct ggcagaaaag gtgaaaacca aagcctgcga tctggtgtta
     3361 aagcaggggc tgaacttcat ttcctgaacc gcgaaatgtt acccggctga cgcggctctc
     3421 tcctgccctt ttccctgctc ctggacggtt ttccccctgt tacacgggaa gcgaccgcga
     3481 tcaaaaaagt ctgtttataa actaattcct aacaacatcg catttttttg acactagaat
     3541 tgcagcaata tggcgacgcc attacattgt gcttctcaaa aagcttaaac gttggctaaa
     3601 cggcccggtt tagtgaggat cgcccattgt cagaactact ctctttcgcc ctttttctcg
     3661 cctctgtgct gatttacgca tggaaagcgg gacgtaacac ctggtggttt gcagccacgt
     3721 taacggtgct ggggctattt gtcgttttaa atatcaccct gtttgccagc gactatttta
     3781 ctggcgatgg tattaacgac gcggttctct ataccttaac caacagcctg accggtgctg
     3841 gcgtcagcaa atacatcctg ccgggtatcg gcattgtgct ggggctgacg gcggtgttcg
     3901 gtgcgctggg ctggatcctg cgccgtcgtc gtcatcatcc gcaccatttt ggttacagcc
     3961 tgctggcgct cttactggcg ctgggttcgg tggacgccag cccggcattt cgtcagataa
     4021 cggaactggt gaaatcccag tcacgcgatg gcgacccgga ctttgcggct tattataaag
     4081 agccgtcgaa aactatccct gacccgaaac tcaacctggt ttatatctac ggcgaaagtc
     4141 tcgagcggac ctattttgat aacgaagctt tcccggatct cacgcctgaa ctgggcgcgt
     4201 tgaaaaatga aggcctggat ttcagccaca cgcagcagct gccaggaacg gattacacga
     4261 ttgcgggcat ggtggcttct cagtgcggca taccgctgtt cgcccccttt gaaggcaacg
     4321 cctccgcctc tgtctccagc ttcttcccgc agaacatctg tctgggcgat atcctgaaaa
     4381 actcgggtta tcagaactat ttcgtgcagg gcgcaaatct gcgttttgcc ggtaaagatg
     4441 tgttcctgaa atcgcacggc ttcgaccact tatacggctc agaagagctg aaaagcgtgg
     4501 tagccgaccc gcactatcgc aacgactggg gattctacga cgataccgtt ctcgatgaag
     4561 cgtggaaaaa gtttgaagag ctttcccgct caggtcagcg attctcactg tttaccctga
     4621 cagtcgatac ccatcacccg gatggtttta tctcgcgcac ctgtaaccgc aaaaaatatg
     4681 attttgacgg taagccgaat cagtcattca gcgcggtaag ttgcagccag gagaatatcg
     4741 cgacgtttat caacaaaatc aaagcgtcac cgtggtttaa agatactgtc atcgtcgtct
     4801 cttctgacca tttagcgatg aacaacacgg cgtggaaata cctcaataag caggatcgca
     4861 ataacctgtt ttttgtcatt cgtggcgaca agccgcagca aaagacgctg gcagtgaagc
     4921 gtaacacgat ggataacggc gcgacggtgc tggacattct cggtggcgat aactatctcg
     4981 gacttggtcg tagcagttta tccgggcagt cgatgtcgga aatcttcctc aatatcaaag
     5041 agaaaacatt ggcgtggaag ccagatatca tccgcctgtg gaaattccct aaagagatga
     5101 aagagttcac catcgaccag cagaaaaaca tgattgcctt ctcgggtagc catttccgtt
     5161 tgccgctgct gttgcgggtt tcagacaaac gcgtggaacc gctgccggaa agcgaatact
     5221 cagcaccgct gcgtttccag ctggccgatt tcgctccacg cgacaatttc gtctgggttg
     5281 acagttgcta caagatggca caactctggg ctccggaact ggcactttcc accgactggt
     5341 gtgtctcgca ggggcagctt ggcggtcagc aaattgttca gcatgttgac aaaacaacat
     5401 ggaagagcaa gacggcattt aaagatacgg tcatcgacat ggcgcgttac aaaggcaatg
     5461 tcgatacgct gaagattgtt gataacgata ttcgctacaa agccgacagt ttcatcttta
     5521 acgtcgccgg tgcgccagaa gaggtgaaac agtttagcgg gatttcccgt ccggagtcgt
     5581 ggggccgctg gtccaacgcg cagctgggcg atgaagtaaa aatcgagtac aagcatccgc
     5641 tgccgaagaa atttgacctg gtgattaccg ccaaagcata cggcaataac gccagccgtc
     5701 ctattccggt acgcgtaggc aatgaagaac aaactcttgt gctgggcaat gaagtgacca
     5761 ccaccacact gcatttcgat aacccaaccg atgccgacac actggtaatt gtgccgccgg
     5821 aacctgtctc aaccaacgaa gggaatatcc tcggacactc gccgcgtaag ctcgggatcg
     5881 gcatggtgga aattaaagtg gtagaacgtg aagggtaatt cctgactgat gtgtaggtcg
     5941 catagggtgc gacctacacg ccttctccgg cttacaacac attattaaga aacaaaaaaa
     6001 acagcgccca acacttcccc ggacgctgca tggcaacttt aataatctcg ccttatcaga
     6061 aagtaattac acccttaatc aactcacgat tgttaatcac atcgcgctcg taggtttctg
     6121 ccagcgtggc gaacggatag cgatgggtta acatcatgtc ggcagtgatt ttcccttccg
     6181 ccatcagtcg accgacttta gcgaaatctt ccggcgtggc gttgcggctg cccatcatcg
     6241 tcgtttcttt tttatggaat tccggatcgg agaactgcaa ctcaccttta aacagaccga
     6301 caaataccac cgtgccgccg tgacgaatca gattcacggt attattcatc gcatgttgat
     6361 tacctgtcgc gtcgatcact ttctgcgcca gcgaaccacc aaactgcgcc cgcagctgcg
     6421 cgtcaaaatc ttcggctgac gggtccagca cgggcaactc cagacgcgtt gccacatgtt
     6481 cacggcgcgc cggactggta tccgccacca ccacctgtgc gccatcggct ttagcgattg
     6541 ccgccgcgcc cagaccgatt ggccccgccc cgaccaccag cacctgctcg ccgggagcaa
     6601 tggctgcgcg acgcaccgca tgagcgctaa tagcgaaagg ttcaatcaat gcggccgcct
     6661 gcgggtcaat accgtctgcg ggcaaaatgt tcgccaccgg cacgctcaaa tactcactaa
     6721 aaccgccatc ctgatgcacg ccaatgactg aaattttttc acagcagttg gtacgcccgc
     6781 ttttacacgc cgggcattgc tgacaggcaa cataagggat cacagcaact tgctgaccat
     6841 ttttaagatc agcaatattt ttacccagcc caacaatctc cccacatatt tcatggccta
     6901 aaacccgtgg ataactaaaa aatggttgat ttccacccta ggcatgaata tcggtaccgc
     6961 aaatcccgac agacttaatt tttattaatg cttcattgtc acccggaata ggtatctcgc
     7021 gttgtttcca gactaattct ttcggctgct ggcaaattaa aacattcatc gtagacatga
     7081 ttcggctatc cattttttgt tgccttagat atcaatgaaa atgaaaaatg gcagcggaac
     7141 ggaaaatctg ttttgtgaaa acacacgcat aaaaatgttt taaatcgggt tttaatggac
     7201 tcacaaggag gaaaatatga gtcgttcaca aaatttacgc cacaatgtga ttaaccaggt
     7261 cattgatgat atggcgcggg gtcatatccc ttccccactt ccatcgcaaa gtgcgctggc
     7321 ggaaatgtac aacatcagcc gcactacagt gcgtcatatt ctcagccaat tacgcgaatg
     7381 cggcgtctta acgcaggttg gaaacgacta tgttattgcg cgtaaacctg accatgatga
     7441 cggtttcgcc tgtactaccg cttcaatgag tgaacaaaac aaagtgtttg agcaggcttt
     7501 tttcacgatg ataaaccagc gtcagttacg ccctagggaa actttttctg aactgcaact
     7561 ggctcgggcc gcgggtgtca gcccggtggt tgtaagagaa tatcttttaa aattcggccg
     7621 ttataatctt attcaaagcg aaaagcgcgg ccagtggagc atgaagcaat tcgatcaatc
     7681 ctacgccgag cagctgtttg agctacgcga aatgctcgaa acacattcac ttcagcactt
     7741 tctcaacctg cctgatcatg atccccgctg gctgcaggcg aaaaccatgc tagaacgtca
     7801 tcggctactg cgtgacaaca tcggcaatag ctttcgcatg ttctcgcaac ttgacagaga
     7861 ttttcactcc ttactgcttt cagctgccga caatatcttt tttgatcaat cgcttgagat
     7921 catctcagtc atctttcatt ttcactatca atgggacgaa agcgatctta aacagcgcaa
     7981 tatcattgcc gtcgatgaac atatgaccat tctcagcgct ctgatttgcc gcagtgacct
     8041 tgatgccacc cttgcgctcc gtaaccattt gaattcagct aaacaatcaa tgattcgatc
     8101 cattaatgag aacacgcgtt atgctcatta agtacggatt aaaaacagca aactattagc
     8161 gggtgcacgg ttttatcaaa agataaatac cgggaaattc ccgcttacct atgctcacaa
     8221 tcagtcgact aaaaatgttt ttgtgagcgg tagtaaagtc ctaaaacttt aacctgccgt
     8281 taatgtcaac cttgcgtaat gcacaattac gtaaacaatt aaaattataa atacaggagt
     8341 ccggcgtgga aaaagaaaat atcaccctcg atccgcgttc ttcatttact ccatcttcgt
     8401 cggcagatat tcccgtgcca ccagatggat tagttcaacg cagtacccga attaaacgca
     8461 ttcaaaccac cgccatgttg ttattatttt ttgcggcggt aatcaattat ctcgaccgca
     8521 gttcgctgtc ggtagcaaat ttaacgattc gtgaagaatt gggattaagt gccaccgaaa
     8581 tcggcgcttt gctctccgtg ttttcactcg cttacggcat tgcgcaactt ccttgcggcc
     8641 cactattgga tcgtaaaggc ccacgcctga tgctgggact ggggatgttc ttctggtcac
     8701 tgttccaggc catgtctggc atggtgcaca gctttacgca gttcgtgttg gtgcgtatcg
     8761 gtatggggat tggtgaagcg ccgatgaacc catgcggtgt aaaagtcatt aacgactggt
     8821 tcaacatcaa agagcgcgga cgcccgatgg gcttcttcaa cgcagcttct accattggcg
     8881 ttgctgtaag cccaccgatt ctggcggcaa tgatgctggt gatgggctgg cgcgggatgt
     8941 ttattaccat tggtgtactg gggatttttc tcgccatcgg ctggtatatg ctctatcgca
     9001 accgcgagca cgtagaactg actgccgttg aacaagctta tctcaatgca ggtagcgtca
     9061 atgcccgccg agatccgctc aattttgccg aatggcgcag cctgttccgt aaccgcacaa
     9121 tgtggggaat gatgctcgga ttcagtggca tcaactacac tgcgtggctg tatctggcct
     9181 ggcttcctgg ttacctgcaa acagcctata acctggattt aaaaagcaca gggttgatgg
     9241 cggctatccc tttcctgttt ggggctgccg ggatgctggt caacggttac gttactgact
     9301 ggctggtcaa agggggaatg gctccgatta aaagccgtaa gatctgcatt attaccggga
     9361 tgttctgttc tgccgccttt acgctgatag taccacaagc gacaacatcc atgatggcgg
     9421 ttctgctgat tggcatggca ctgttctgta ttcactttgc cggaacatcc tgctggggct
     9481 tgatccacgt cgcagttgct tctcgcatga ctgcgtcggt gggcagtatc cagaactttg
     9541 ccagcttcat ctgcgcctct ttcgcgccga tcattactgg ttttattgtt gatacaaccc
     9601 actcattccg tctggcacta atcatttgcg gttgcgtcac cgcagcggga gcactggcgt
     9661 acatcttcct ggttcgccag ccgatcaacg acccgcgtaa agattaaact ccctatgccc
     9721 gataacattt tgcttatcgg gcatttttat ggcgattaaa acgtttccca gttctccccg
     9781 ctatctgcca ctgccatttt acgcggcgta gctggcgtca cggtttttac cacagccgat
     9841 gtttcacgct gctgttgctg ctgttgctga atccggaaca ctgccacagc ttcggtcagg
     9901 cgactggcct gctcttccag cgcggcggcg gcagcggcag attcttccac cagcgcggcg
     9961 ttctgttgag ttacccggtc catctcagca accgctaagc caacctgatc gataccacgg
    10021 ctctgctcat cagaagcaga agctatttcg cccataatgt ccgtcacgcg ggtcacagca
    10081 ctgacaatct ccgccattgt ttccccggcg ctttcgacca gcgtagagcc aacatccact
    10141 ttccccaccg agtcttcaat caggctttta atttcacgaa ccgcctgggc gctacgctgg
    10201 gccagattac gcacttctcc cgcaaccacc gcaaaaccac gtccttgctc acccgcacgc
    10261 gctgcttcaa ccgccgcgtt caaagccaga atattggtct ggaaggtaat gccgtcaatt
    10321 acgctgataa tatcggcgat tttctgcgaa ctggtggaga tatcgcgcat agtctgcacc
    10381 acgttatcta ccactttgcc gccgcgttgc gccgtttcag aagcacttaa tgccagatgg
    10441 ctggcctggc gcgcattctc ggcgttctgt ttcaccgttg cggtcagttg ctccatgctg
    10501 gctgccgtct cttccagcga agcggcctgt tgctcggtgc gcgaagagag atcattattg
    10561 ccggtagcga tttcgctggc accgctatag atggcattgg ccccgttgcg cacatcaccg
    10621 acggtacgca tcagctctcc ctgcatatgg cgcaaactct ctgccagttg ccccatctca
    10681 ttagagccat ccacctcaat cggtttcacc agatcgccgc ctgcaatatg acgaatgctg
    10741 tcaatcaggc gattcattgg cgctaccagc gaggctttaa taccgaacca gacggcgaag
    10801 atgaccgcca gtacgacgat catcacgccc accagaatcc acatcgcctg gctgtaggag
    10861 gcattgttat cgctgacggc gatatcatag agccgatcgt tttgctccat gtaagccaca
    10921 tactgcttct cgaaaccgtc ctgatatccc tgggtcggct gatcaaagaa ctcgttgatt
    10981 ttgcctgcac ctaacagttg gatcagctcc gccagcgcat tgtgataaat atcgtaatta
    11041 cgtttgatct ctgccgctgc ggcggtgctc tgacgcgggt cacgcggcaa cgcttcgtaa
    11101 tccgcccagt ttttttccgc ctgtttcagc gaaatactgg cactctgcat cagctcagca
    11161 acggttgaac cgctaccaat attattctga tccatcatgt agcggatacc cgcgcggttg
    11221 agggtgttac gcgtctgcaa caacgcgacc cagctgccat tcagcgtggg ttgctgctgg
    11281 cgaatggttt gtaaaacagt gaaattttct ttgtcattct ttaaggcatt aaaaaacaga
    11341 ccgcctgatg tcagttgtaa aaggccaaaa acggccaaaa ccagcagtaa gctggtcacg
    11401 atcttgatac gttttaacat ggtttctctt tcctgtggac cgatgcaaga ttttcggcct
    11461 ggaaagcaaa aactttatga aaaggtgtaa cccgccatcc atggaaaaac caggcgctgg
    11521 ttatcagata ctaaaaagtt attcattatc gcccgggatg tcttcttgtc gggaattgcc
    11581 cagagcaata aattcctcta acagatgggt taactgttgc attttctcgg cagtaaactg
    11641 agcttcaatc tgccgatagg cttcttcaat ctgtgtctga gcgcggttat acagcgcctg
    11701 cccctctttg gtcagcgaga tatagagctt ccgctgatca ttgatcggct ttaatcgcaa
    11761 cactaaaccg tcgcgttcca tccgcgtgag gatcccggtc aggcttgggc gcaaaatgca
    11821 ggcgcgatac gccagatcgt gaaaatccat tgatgggctt tccgccagaa tacgcacgat
    11881 gcgccactgt tgctcagtca aattatgccg tttaacgatt gggcgaaaat aactcatcgc
    11941 cgcttctcgc gcctggagca acgcaatggt tagtgagtcg tgcattatct ttcccctgaa
    12001 a
//
LOCUS       DAZPCS010000111        11913 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZPCS010000111 DAZPCS010000000
VERSION     DAZPCS010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11913
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.111"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..2251)
                     /locus_tag="ACHUQ7_002913"
     CDS             complement(<1..2251)
                     /locus_tag="ACHUQ7_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HFR1136227.1"
                     /translation="MGTGLAKADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELHLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGQQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTEGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFRDPLHSSPIVSAPAKLSVLDANGSLLQTVDVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     NARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDASGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGELPSGGKSFTVNFAKPGNYNLTLRDKDGLILAGLSHAVSGKGST
                     AHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTLYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVE"
     gene            complement(2275..2898)
                     /locus_tag="ACHUQ7_002914"
     CDS             complement(2275..2898)
                     /locus_tag="ACHUQ7_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HFR1136228.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSRLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGSGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(2895..4583)
                     /locus_tag="ACHUQ7_002915"
                     /pseudo
     CDS             complement(2895..4583)
                     /locus_tag="ACHUQ7_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
     gene            complement(4732..7359)
                     /gene="gyrA"
                     /locus_tag="ACHUQ7_002916"
     CDS             complement(4732..7359)
                     /gene="gyrA"
                     /locus_tag="ACHUQ7_002916"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFR1136229.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7506..8228
                     /gene="ubiG"
                     /locus_tag="ACHUQ7_002917"
     CDS             7506..8228
                     /gene="ubiG"
                     /locus_tag="ACHUQ7_002917"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFR1136230.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(8356..11913)
                     /gene="yfaL"
                     /locus_tag="ACHUQ7_002918"
                     /pseudo
     CDS             complement(8356..11913)
                     /gene="yfaL"
                     /locus_tag="ACHUQ7_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
BASE COUNT         2754 a         3303 c         2943 g         2913 t
ORIGIN      
        1 attcgacatt gaccagttca ccaggctggt aatgggtttt gtccgttttc acccggatat
       61 caagctgagg aacggcaact ttgatcccgg cgttctgaaa actgtactga ccattacggg
      121 tatacagcac cgaaaaagtg atgttaggcg caaaggaatt gctcactggt acccgggctt
      181 catacagggt atcgtttaaa cgttgtagcg ttagccagtt tgccggatgc gaaagcagcg
      241 actgctgttc cacgcgatcg cgttccagcg tcaataacgc ttcatcaatt ggctccggga
      301 aagtaatcag catcttcgcg gtttcgcctg gctggtacag cgttttatcc gccacgatat
      361 ctaccgtacc agtatgcgcc gtgctgccct taccgctgac ggcatgactt aacccagcga
      421 gaattaagcc gtctttatcg cgtaatgtca gattgtagtt gccaggttta gcgaaattga
      481 cggtaaagga tttgccgcct gacggtagct ctccgctatg gctcgtgcgg tcttcgagac
      541 gcaaccattc atacgtaaca ggaacctgtt ttgaagattc cagcgcggca taacggaaca
      601 caaccgactc gccgctatta ctgtattgtg cggcggtact taaagagtaa tgcgccaggc
      661 cgcgttcaat gaggatctct ttggtggtgg tgacgcgata cgccgcgccg tcactggcgg
      721 agacggttaa caaatagcgg ctcggtttat cggcggcggg gagatttaac gccacatgac
      781 cgctggcgtc ggacaccgtt tcgctgcctt ccagcgacac ggggaaacgt ccggcataac
      841 gcaaatcgtt accgaccatt gataattgct gagcgcgcaa acttaactgc acgcgggcat
      901 tttttaccgg ctcgccatcc gggtagagca gttgcagttt gccgctgacc gcttcgccag
      961 ttttgaactc ttttttgtcg agagctaaac caatctcgaa atgtggcttg atgtagtttg
     1021 ccacgcgaaa actgctgcta tagacctgat tgcggtaagc aagacgtaac tcataacctc
     1081 cggcaacggc attttctggc aggcggaaac ttccctgccc gccattgcgc gcatccagcg
     1141 tgacatcgac ggtttgcaac agactgccgt tagcgtccag caccgaaagc ttcgccgggg
     1201 cgctgacgat gggggatgaa tgcaacggat cgcggaactc gcggcccatc actttaacat
     1261 cgacacgatc gcctgcgcga tatagcgggc gatcggtaaa aatatacaag cgggtgttgt
     1321 agatttcgct ttcgtagaag aagttctcgg agacaaaaac gccgccttca gcatccttac
     1381 ccagaatgta tgaacgttct ggcgatatat gttgtaactg caaagtaccg ctgtcatcgg
     1441 tcaccccgcg ggtcatcacg ccaagacctt cagtccacaa gatctctgag ccgggcttcg
     1501 cttcaccctg ttttttaccc gcggtccata ccagaagctc gttacctgac actttgctaa
     1561 gcgccacggt atcggaaaca aacaccaccg tcgtcgcccg atacccacct accatcgcct
     1621 cgacgaggta cagccccggc tcttgttggc cgagaggaat ataaatatta cccggctgcg
     1681 gcgagatgaa attgctggat gcgccttcca gttttacccc ttgctgcggc tcgaccggtt
     1741 tagcctgcca tagtggataa cggaactgtt ccaccaaggg atattttttc agcggggaaa
     1801 actggttgtt ctgtacataa cgggaaggtt taataatggc attgccgaga tgtaattcgg
     1861 gtaatgcctg agtcacattc tgccgtgact gagaagagaa agtacgctgc atcacgcggc
     1921 gagatttgcc gtaccagtta tcccacagcc aggtcagcgt attgttcagc ccgtcgccca
     1981 gatattgcgg ttgcaccaca atgcgatgca ggtttttctg ctggcgcaaa aatgccatcg
     2041 ggtcaggaat acgatatagg cgaacgtcca cgccgccgta ctcttccatc tgatagcgcc
     2101 gataatcacg ccccggcgct tccagtcgca ctttcgcctc ttcactgctg ctaaaactgc
     2161 tgtcagcaag caaaaagaat gttcccccgg cgggcggcgc atagttactg gaaggaagcg
     2221 aatcatcagc tttagcaagc cctgttccca caagagacag ggacaagcac gctgctatcg
     2281 cgccagaaaa tttaaacgat aaatgccaat gaaattggga ttggaatcgt tgggtatcca
     2341 tcgggtgtcc ttccatgtca taagttgttg cagactgact gcgcgcattc cgttgtcagt
     2401 tttcgtggcg cttccggtgt ggtagatgac gtaacgcccc atccagacca ttaagtgctg
     2461 ggcatcgccc tgatcgaaaa aaatcatatc gccaggcagc gcctggttta tgtcttggcc
     2521 aataaactgg ctgttgtact gaatcaaatt aatcgcggtc acgtaggggc cgtttttccc
     2581 gctcccctga ttccagtttt gcgccagttg acgctgttca ggtgttagcg tcatctctgg
     2641 cggcaaatac tggctagata aaccgttact ttttagccat ttactgtcgt gaactttcag
     2701 cgtctcgttc gccgcaaatc gcaccaggcc cgcacaatcc tgctgatacc agcgtggact
     2761 tggcccctgg cggagctgtt cttgtgcaat gcgtacaaac caggcgcgaa acagcctgga
     2821 ttgttcgaca ttcagcattt cgctatgggc aacaacacaa cacagccagc aaatcagcgc
     2881 cagcagcccg tgtctcatag cggctgccag gttatgggta gccactgcca ggcgacaccg
     2941 ggttccatct gggccagctt catgacataa cgcggttgtt gagataaagc gtccagcttc
     3001 ggcattaata aagtttgtgc ggcgttataa aaaaccggtt cgagattctt cggcagactg
     3061 gtcaacgttt cgttacgcag cagtttcgcc acgccttgtg gattgatata gagcggaacg
     3121 atgccatcag tgggtattac atccaccatt gccgggcggg ttttattcag tgtttgcagg
     3181 gcgttattaa ccagcgtgtc atcgagggag aaaagcagcg ttttgttttg catcgccagc
     3241 gacacacgga aaaaataatc cgacattaat tgatcgggtt gcgccgcctg cgctttcgga
     3301 tactgaccgt atcgggaact cacttcgcga caccagattt gcgcttcgcc ctgctgagtc
     3361 tggcttaccg gcaaaacacc ttctggcgct ttgctttcgt gcgcaccaat attttgcgta
     3421 aacagtttcc ctggcaattg cgcctgttcg gcagtgccat caaactgacc gacaaacagc
     3481 ggggtttgca attttgagtc ttcataccag cacagccccg cggcaccgtc taacgcccca
     3541 ttcagcttgt cgttttcctg gctgatgtgc gaaagcatct cttcggcaat accgtgtgaa
     3601 tacggcaccg ccacacagaa gctggctccg gcaggcatac tgttccagac cggcgtaaaa
     3661 tcgaaactgg catctacgct ggcggattca tcatttaacg ccacaaaact gtgccagccg
     3721 tcgttaccca tttcgaagcg tacgccagca aaagaaggca ttaaccgctg gtagccaaaa
     3781 cccagcaacc tggcgctgac cacgatgcgc aggcgtactg gcgttttttc ggctgtacgc
     3841 tcttccaggc caaagctggc ttgccagcgt tttttaccgc tcaacaaatc acttgcgatc
     3901 gccgtggctt cggtatcctg ctgatcatct ttaaacaaca tatccgtact ggaaaacacc
     3961 agcatcttgt cctgatatgt cgcgaacatc agggcgttat tgccgttata gcgcaactga
     4021 taaacaggaa tagtttcact atttatcttg atgctactga tttccgtttt gcttaactag
     4081 ctgtcgctgg tagcggcaaa caacaatggc tccagcagtt tgcttaaacc actgcgctgg
     4141 atcagcacca tgtaatgtga aagatggcct tgtttatcgt gccatagcgc cgcctgcgcg
     4201 ggctgatcta agagtgacga aaagagctta tctttcagcg tgagatcgtg ttcatagaca
     4261 atgcggcgaa tgctgccttc aatgcccaga cgatcggcat gattctgata atagaaaacg
     4321 aaatcttcgc tcagaacatc gtggagaaac ggaatggtga ggagatcttt gggaagctgg
     4381 ctcagagagt cgctgtctag aaagaggtcc ggctcattga gatcgatttg cagattgttg
     4441 tgcaccacca gcggcgacaa cgttttttct ggcccactgc cagcatattg caacgcccag
     4501 acgccagcgg aaagcagtgc tattgcgcca aaacctacaa gaccatagaa ccgccagcct
     4561 ttcgcctttt tttcaccact cattgccaca ttccttgtgt atagccagcc attttttacg
     4621 ggcacagcca aactttaccg cgccctaata cgacaaaagc ccagactttg cagcctggac
     4681 ttttcaattc aaacaaggga gatagctccc ttttggcatg aagaagtaaa attattcttc
     4741 ttctggctcg tcgtcaacgt ccacttccgg agcaatttca tcatcccctt ccgcggcact
     4801 gccgtcgatg gtatccagat cttcctcgtc aaccggttca gcaacacgct gcagacccac
     4861 tacgttttca tcttccgcag tacggatgag gatcacgccc tgggtgttac ggcccacgat
     4921 gctgatttct gaaacgcgag tacgtaccag cgtaccggca tcggtgatca tcatgatctg
     4981 gtcgcagtca tctacctgca ccgcgccaac cactaaaccg ttacgttcgg taaccttgat
     5041 ggagataacc cctttcgtcg cacgcgactt ggttgggtat tccgccactg cagtacgttt
     5101 accgtaaccg ttttgcgttg cggtgaggat tgcgccatcg ccacgaggca cgatcagaga
     5161 gacgacttta tcgccttcac ctaagcgaat accgcgcaca ccagtggtat tgcagcccat
     5221 cgcacggaca gaagactctt taaagcgcac cactttacct tcggcggaga acagcattac
     5281 ttcatcttcg ccactggtca gatcaacgcc gatcagctca tcgccgtcaa ccagtttgat
     5341 cgccacttta ccggcggtac gcagacggtt gaattcggtg aggacggttt tcttcacagt
     5401 accgttagcg gtcgccatga agactttcac gccttcttca aactcggtca ccggcaggat
     5461 cgcggtgata cgttcgtcct gttccagcgg tagcaggttg acgatcggac gaccgcgcgc
     5521 gccacgagtg gcttccggca actggtaaac tttcatcgaa tagacgcgac cacggctgga
     5581 gaagcacaga atatggtcgt gcgtgttcgc caccagcagt cggtcgataa agtcttcttc
     5641 tttaatacgt gcggcagatt tacctttccc gccacgacgc tgcgcttcgt attcagaaag
     5701 cggctgatat ttaacgtagc cctggtgaga gagcgtcacg accacatctt cctgggtgat
     5761 cagatcttcc aggttgatgt ctgcgctgtt ggcggtgatt tcagtacgac gtttgtcacc
     5821 gaactgttca cgaaccagct ccagctcttc gcggatcact tccatcagac gatcggcgct
     5881 accaagaata cgcaacagtt ccgcgatctg atccagcagc tctttgtatt cgtcgagcag
     5941 tttttcgtgc tcaaggccgg tcagtttctg caaacgcaga tccagaatcg cctgagcttg
     6001 ctgttcggtc aggtagtaca gaccatcacg cacgccgaac tctggctcca gccattccgg
     6061 acgcgcagca tcgtcgccag cacgttccaa catggcggca acgttgccca gctgccacgg
     6121 attagcaacc agcgcagttt tcgcttctgc aggcgtcggc gcatgacgga tcagttcgat
     6181 gatcgggtca atgttcgcca gcgccacggc taatgcttca aggatatgag cacgatcgcg
     6241 agctttacgc agttcgaaaa tagtacgacg ggtcaccact tcacggcggt gacgaacaaa
     6301 cgccgcgatg atgtctttca ggttcatgat cttcggctga ccatggtgca atgccaccat
     6361 gttgataccg aaagaaacct gcaactgggt ctgggagtag aggttgttga gcacaacttc
     6421 accgaccgca tcgcgtttca cttcaatcac gatgcgcata ccgtctttgt cagactcgtc
     6481 acgcagcgcg ctgatgcctt ccacgcgttt ttcttttacc agttccgcaa tcttctcgat
     6541 caggcgcgct ttgtttacct gatacggaat ttcgtggacg ataatggttt cacgtccggt
     6601 tttggcgtca acttccactt cagcacgggc gcggatatat accttgccgc gaccggtacg
     6661 gtaagcttct tcaataccgc gacgaccgtt aatgattgcc gccgtcggga agtccggccc
     6721 cgggatgtgt tccatcagcc cttcaatgct gatgtcttca tcatcgatat acgccagaca
     6781 accgttgatg acttccgtca ggttgtgcgg cgggatgttg gttgccatac ctacggcgat
     6841 accggaagaa ccgttcacca gcaggttagg aattttggtt ggcatgacgt cgggaatttt
     6901 ttccgtgccg tcatagttat caacgaaatc gaccgtctct ttttcgagat cggccatcag
     6961 ttcatgggca attttcgcca gacggatttc cgtataacgc attgccgccg cagagtcgcc
     7021 gtcgatggaa ccgaagttac cctgaccgtc taccagcatg taacgcagcg agaatggctg
     7081 cgccatacgg acgatcgtgt cataaaccgc cgagtcacca tggggatggt atttaccgat
     7141 tacgtcacca acgacacggg cagatttttt ataggctttg ttccagtcat tgcctagtac
     7201 gttcatggcg taaagtacgc gacggtgtac cggcttcagg ccatctcgga catctggcag
     7261 cgcacggcca acaatgaccg acatcgcata atccagatag gagctcttta gctcttcctc
     7321 aatgttgacc ggtgtaattt ctctcgcaag gtcgctcatc taaccgctat ccctctactg
     7381 tatcccggat tcaaaggtcg caaattataa cacagccgcg cagtttgagg taaacctata
     7441 cgctttattc acatccaatg cctgatatac tcgtttgtct tgccaattac ggagtagaag
     7501 tgccaatgaa tgccgaaaaa tcgccggaaa accataacgt agaccacgaa gagatcgcta
     7561 aatttgaagc cgtcgcctcc cgctggtggg atcttgaagg tgagttcaaa ccgttgcacc
     7621 gcattaaccc gctgcgtctg ggctatattg ccgagcgtgc tggcggttta tttggcaaaa
     7681 aggtgctcga tgtcggttgt ggcggcggca ttctggccga gagtatggcg cgcgaaggcg
     7741 cgacggtgac cggtctggat atgggctttg agccattgca ggtggcaaag ctgcacgcgc
     7801 tggaaagcgg cattcaggtg gattacgtgc aggaaaccgt ggaagagcac gcggcaaaac
     7861 atgccgggca gtatgatgtg gtgacctgca tggagatgct ggagcacgta cccgatccgc
     7921 agtcagtggt cagagcctgt gcgcaactgg tgaaaccagg cggcgatgtc tttttctcga
     7981 cgcttaaccg caacggcaag tcatggctga tggcggtggt tggcgcggaa tatattttgc
     8041 gcatggtgcc caaaggtacg catgatgtga agaagtttat taaaccggca gagctgcttg
     8101 gctgggtgga tcagaccagt ttaaaagagc gacatataac cgggctacat tacaacccga
     8161 tcactaattc ctttaagctc ggccccggcg tggatgtgaa ctatatgctg cacacgcaga
     8221 ataagtgagg ttgatgtttg gccgcgccaa tgcctgatgc gacgcttgca gcgtcttatc
     8281 aggcctacaa atgctctccg taggccagat aaggcgttta cgccgcatcc ggcaattgtg
     8341 atggtcagac agcgattacc atttcaccgt catcgacaaa aaccctgccg tctgggcaaa
     8401 atcatcactc cctttctgcc acgccacgct gccgcgcagg gacactcgct gactgatatt
     8461 gcccgtgact cccactttta tttcaccccg tagcttcacc gcatcgtcac tgatagtgct
     8521 gccatcttct tcaatttccg tcgcatgggg atcgtgatag taattcagat ctaatgttgg
     8581 tgtgacacca accgcggtac gccattcgct gtgtaaaccc agccgcgtct gaatatcatc
     8641 cccctgcgat tgtgacacgc gcgcatggtt agcggcggta aaatcatcct gctgcacgcc
     8701 ctgataaatc acctgcgcct gcggttcaat caccacacca cgccccggta accactgata
     8761 cccagcttcc agcgaggcga taatccccga cgaatggtaa cgatccacgc catcttcatg
     8821 ttcggaaaca tcattgttaa accacgcgta ttgcagccag gccccctgct tctgattacc
     8881 gtgctgatac cagcttgagg tcagcccaac ggcataaccg tggttctggt tatcggcgcg
     8941 agttccggtc atattcgagc ggctgtcgcc ctgattatca ctgtagccac caaccgcccc
     9001 aagcatccac tcgccatccg tgccccagcg cccgctaaac aagccgccgc taagctgcac
     9061 cgtagaagtg tcttcctgct gagccagttg ccctgccgcc gtgtaatgat aacgtccgcc
     9121 gataacacgt aaattcagcg tctgaccatc gccaccagcg tgatcgcgtc gctccatcat
     9181 aaacgcctga tttgccgccc gcaggttatt gagataaccg ccaactttgg cattcagcac
     9241 cggctggtaa gcaggcgtag gggccgggtc gggggttggg tcaggatccg gcgtgggatc
     9301 aggatcggga gtcgggtctg gctcaggtgg cgatggcggc gttacttctt gcgatcgcag
     9361 ataccagtcg ttgttatctt ccactagcgt gtagtcatac gctcccatgt tgacgtagcc
     9421 actgccagcc agactgaact gtgcattgtt ttgaaactgc gtgggatcag ctgcgaaatc
     9481 aaccacttta atgcctgtcg atgtcggctc accaatccct gtaatggagt taaccaccac
     9541 agttgtgttg ccagcagtat taccgttcat caccaattga tcgcttaccg agtcatcgcc
     9601 gtttaattcg ctgtcgaata gtagcgtacc gccgccggta taatcaccgt ttaccgtcag
     9661 cgtatcgcca gcgacgccat tttgcaggct aaccgcaccg gacgtattgg tcagatcacc
     9721 attaaccgtc acgtcagcaa gataggtcag gctgtcagtt tcaaggttgg tattaagctc
     9781 gcctgtcaac gtcacgccat cggtagcatt taccagcgtc ccatcgccgt taaatagtga
     9841 ggcattaagg gcgacggaag tatcctgccc ggtcagacgc aaaaccgtac catcgctgat
     9901 gtcgatagtg ccgctggaag tagcatcaat tgactgaata tcctgatccg cgccagtaac
     9961 gaacgttgcc ccatcaccaa cccacagtga cgaagcataa ggaaagatat ccgcaacatc
    10021 gcctttcagg gtgccttctt cgacacgtac cgccgactgc gttgtaccgc tggcggtcag
    10081 ctccagcgta cccgcccccg ttttagtaaa tgtgctaccc tcatcctgat gctgcccgct
    10141 gctgtcagcc atcagtgcgc cccactgcgt gtctaccccc gcatcaactg ccacttcacc
    10201 gtcggcgcgc atttcaatat cacgcccatg ctcagtcgtc gtagagcgag tatcgacatt
    10261 ggcagtaatc tccatcacgc tttcttgtta cttatcggta aagataacct ggcggttata
    10321 gtgggtatct ccaagctgcg agttgtcgct caccatcagc gtaccggagg cgatttgtgt
    10381 tgtgcccaaa taactattat tgtttgccag acttacctgc ccgtcgctcg catcgaccac
    10441 cacgtagacg ccatctttcc cgtcgccgcc gatatgcaaa ttgtcgccac ctgcaagatc
    10501 taccacatcc tgactgccga taaccgttcc gctgctgcca ctgacttcaa ggccatcatt
    10561 gtatgatgtg ccgctctgcc aagtggagaa atcagagaga tcgagcacac cgccgtttaa
    10621 cacgatcgac tgcggatcgt cctggagagc ggtaagatct gccgcgtcgc cttccagcga
    10681 aagcaccgca ccatcatcaa cgactatatc gccggttagc gccatcgact gcgcccctgc
    10741 cagcacgtag ctgccgtttt gcgcaatggt gagctgacca gcaccttcga tgatgccagc
    10801 aaaactgccc tgattaacag taacgttgcc accagcatcg atatttaaag tgccattctg
    10861 aaagcccgtc aatgcgtgca caaaagtttg ttgggtcgag ccaacgttta gctcagcctg
    10921 attctgatac tgatcaatac tccctatcgt cagaccgtag cagtcttgcg gatcgtcctg
    10981 gcaatgcgta tcgccgacat tcatcaggga gttgctgcga cccagggtaa cttcaccgtt
    11041 ttcaatctgc atctcaccag taaagtcatt gttatctgca ttaagtacca gatcgccgga
    11101 acctgttttg gtgattaacc cggtaccagc aatagagtca acagctccgt cattctctgt
    11161 attgccgata accagcgttt ttccgtcggc aatatcaaag gtaacttcac ttaagccgag
    11221 atacataaag ccacccgccg cagtggaggg accatctcca tagcctgctg cgctattgtt
    11281 ctcatcgact aacacgccgc cgttctggct gtagctgtca tcaacagaaa tatcaataag
    11341 atagggagcc gtcgcgctat tggtatatat cgccccgcca taaccttcgg ctgtgttatt
    11401 tgtaaaggcg gtgttattta ttatcgtata acctgaagga tgcttgctgt cgctattatt
    11461 atcggtaaca tcgattgccc cgccatcgcc atcactgtaa cttgttgatg tatatgcctg
    11521 gttattgtta aaaacatcat cacttaaata gacgtcatta ttaatggtat aaattgcgcc
    11581 acctttgcca tcattagcga tattattgat ggcgttagtg acacgtaaat cgacggcacc
    11641 agtatcatta gtaccagaag aatagattgc gccaccatag ccgcctgcga cgttaccgga
    11701 aaaaataaca tcagtaagat tcagcgttga gttttcttta gcaaatatgg ccccgccgtt
    11761 attatattct cctgtaacgg tgttattggc aaacagagtc attccagttt cattttctgg
    11821 taatagtgaa aattccgctc cttgttgcaa aaatacggcc ccaccgctgg cattattggt
    11881 catatccgaa aaaaccagcc attgcccatc ggc
//
LOCUS       DAZPCS010000112        11827 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZPCS010000112 DAZPCS010000000
VERSION     DAZPCS010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11827
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.112"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(62..802)
                     /gene="cysQ"
                     /locus_tag="ACHUQ7_002919"
     CDS             complement(62..802)
                     /gene="cysQ"
                     /locus_tag="ACHUQ7_002919"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFR1136231.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            992..2935
                     /gene="cpdB"
                     /locus_tag="ACHUQ7_002920"
     CDS             992..2935
                     /gene="cpdB"
                     /locus_tag="ACHUQ7_002920"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFR1136232.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VIVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIAPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(3061..3441)
                     /locus_tag="ACHUQ7_002921"
     CDS             complement(3061..3441)
                     /locus_tag="ACHUQ7_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFR1136233.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            3530..4390
                     /gene="qorB"
                     /locus_tag="ACHUQ7_002922"
     CDS             3530..4390
                     /gene="qorB"
                     /locus_tag="ACHUQ7_002922"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFR1136234.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGCPTTTLAE
                     SVSHLFNVNN"
     gene            4499..5464
                     /locus_tag="ACHUQ7_002923"
     CDS             4499..5464
                     /locus_tag="ACHUQ7_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR1136235.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            5572..6234
                     /gene="ytfE"
                     /locus_tag="ACHUQ7_002924"
     CDS             5572..6234
                     /gene="ytfE"
                     /locus_tag="ACHUQ7_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFR1136236.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(6279..7691)
                     /gene="cycA"
                     /locus_tag="ACHUQ7_002925"
                     /gene_synonym="dagA"
     CDS             complement(6279..7691)
                     /gene="cycA"
                     /locus_tag="ACHUQ7_002925"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFR1136237.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHVQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(7999..8619)
                     /gene="fklB"
                     /locus_tag="ACHUQ7_002926"
     CDS             complement(7999..8619)
                     /gene="fklB"
                     /locus_tag="ACHUQ7_002926"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR1136238.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            8838..9383
                     /locus_tag="ACHUQ7_002927"
     CDS             8838..9383
                     /locus_tag="ACHUQ7_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OapA family protein"
                     /protein_id="HFR1136239.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNASVVTREAQLDIQSQSQPPTEEQPQTTQTQPFQPDSGIDNQWRSYRV
                     EPGKTMAQLFRDHGLPATDVYAMAQVEGAGKPLSNLQNGQMVKIRQNASGVVTGLTID
                     TGNNQQVLFTRQPDGSFIRAR"
     gene            complement(9367..10014)
                     /locus_tag="ACHUQ7_002928"
     CDS             complement(9367..10014)
                     /locus_tag="ACHUQ7_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFR1136240.1"
                     /translation="MYIDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKYNIFKPGIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            10226..11017
                     /locus_tag="ACHUQ7_002929"
     CDS             10226..11017
                     /locus_tag="ACHUQ7_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HFR1136241.1"
                     /translation="MKSTRPVAVITGATRGIGKGCAQELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            11027..>11827
                     /locus_tag="ACHUQ7_002930"
     CDS             11027..>11827
                     /locus_tag="ACHUQ7_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFR1136242.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQITRQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLMPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDINS"
BASE COUNT         2985 a         3074 c         2988 g         2780 t
ORIGIN      
        1 tttaggttgg gataaatcag gatggcagaa tcaggaaata cactgtttct gccatctgaa
       61 tttagtaaat ggacactctg aaccccggat tcaggaacga ctcgcgcgga gtgtaatcca
      121 gcggtttacc ctgccagtcg tgaacgtgcg ctccggcagc tgcagccaca gcatgtccgg
      181 cggcggtgtc ccaaatattc gttggtccga agcgcgggta cagctgcgcc tgtccttccg
      241 ccaccaggca gaatttcagc gaagagccga tggacgtggt ctgatgttcg ccaagctgtt
      301 gcagatactc tttcagctcc gcatctgcat gggaacggct gataaccacc agcggcgggc
      361 gcgcatcgcg gacctgaatc agcttgcgca caccgcactc ttctttccag gccttgcctt
      421 ctgccgcgct gtacattacg ttcattaccg gcgcatacac cacacctaaa atcggtttgc
      481 catggtcaat gagcgcaatg ttaacggtga attcgccatt acgtttaata aactctttag
      541 taccatccag cgggtctacc agccagtaac gctgccagtg ctgacggact tcccaaccgg
      601 gaggatcttc ttcagaaagg accggaatat ccggtgccag cgtacgtaaa ccgtccatga
      661 taacggtgtg agcggcaata tccgctgccg ttaccggaga attgtccgct ttgctgacga
      721 cgtccatcgg tttcgtcccg tcgtagacct gcataatggc atcgcctgca ttccgtgcaa
      781 gctggcatac ttgatctaac atttctccac ctcgtctctg tgagcggtgt taacttattg
      841 ttttacttat accctatcgt taatgaatgc gccaactgtg atagtgtcat cattttcaaa
      901 gcgtaaaatt gtggcattct tcactgttct ataagtaagg cgtttattct cccttttctt
      961 tcgtattccc gatgataaaa ggatgtccct gatgattaag tttagcgcaa cgctcctggc
     1021 cacgctgatt gccgccagtg tgaatgcagc gacggtcgat ctgcgtatca tggaaaccac
     1081 tgatctgcat agcaacatga tggatttcga ttattacaaa gacaccgcca cggaaaaatt
     1141 cggactggta cgtacggcaa gcctgattaa cgatgcccgc aatgaagtga aaaacagcgt
     1201 actggtcgat aacggcgatt tgattcaggg gagtccgctg gccgattaca tgtcggcgaa
     1261 aggattaaaa gcaggtgatg ttcatccggt ttataaggcg ctgaatacgc tggattatac
     1321 ggtcggtaca ctcggcaatc atgaatttaa ctacggtctg gattacctga aaaatgcgct
     1381 ggcgggagcg aaattccctt atgtaaatgc caacgtcatt gacgccagaa ccaaacagcc
     1441 aatgtttaca ccgtatttaa ttaaagatac cgaagtggtc gataaagacg gaaaaaaaca
     1501 gacgctgaag attggctata ttggcgtcgt gccgccgcaa atcatgggct gggataaagc
     1561 taatttatcc ggaaaagtga cggtgaatga tattaccgaa accgtgcgca aatacgtgcc
     1621 tgaaatgcgc gagaaaggtg ccgatgtcat tgtcgttctg gcgcattccg ggctgtctgc
     1681 cgatccgtat aaagtaatgg cggaaaactc agtttattac ctcagtgaaa ttccgggcgt
     1741 taacgccatt atgtttggcc atgctcacgc cgttttcccg ggtaaagatt ttgctgatat
     1801 cgaaggggct gatatcacca aaggcacgct gaatggtgtt ccggcggtaa tgccgggcat
     1861 gtggggcgat catcttgggg tggtcgactt acaactcagt aatgacagcg gtaaatggca
     1921 ggtgacgcag gcgaaagcgg aagcacggcc gatttacgac atcgccaata aaaaatccct
     1981 cgcggcggaa gacagcaagc tggtagaaac actcaaagcc gatcacgatg ccacacgcca
     2041 gttcgtcagc aagccaatcg gtaaatctgc cgacaatatg tatagctatc tggcgctggt
     2101 gcaggacgat ccgaccgtgc aagtcgtgaa caacgcgcaa aaagcgtatg tcgaacatta
     2161 cattcagggc gatccggatc tggcaaaact gccggtgctt tcagctgccg caccgtttaa
     2221 agttggtggt cgcaaaaatg acccggcaag ctatgtggag gtggaaaaag gccagttgac
     2281 cttccgtaat gccgccgatc tttatctcta ccccaatacg ctgattgtag tgaaagccag
     2341 cggtaaagag gtgaaagagt ggctggagtg ctccgccgga cagtttaacc agattgctcc
     2401 taacagcacg aaaccacagt cactcatcaa ctgggatggt ttccgcactt ataactttga
     2461 tgttattgat ggtgtgaatt atcagattga tgttacccaa cccgcccgct atgacggcga
     2521 gtgccagatg attaatgcca atgcagagcg gattaagaac ctgaccttta atggcaagcc
     2581 gattgatccg aacgctatgt tcctggttgc caccaataac tatcgcgctt acggcggcaa
     2641 atttgccggt acgggcgaca gccatatcgc ttttgcttca ccggatgaga accgctcggt
     2701 gctggcagcg tggattgctg atgagtcgaa acgtgcgggg gaaattcatc cggcggcaga
     2761 taacaactgg cgtttagcac cgatagctgc cgataagaaa ctggatatcc gtttcgaaac
     2821 ttccccgtca gataaagccg cagcgtttat taaagagaaa gggcagtatc cgatgaataa
     2881 agtcgcgacc gatgatatcg ggtttgcgat ttatcaggtg gatttgagta agtaaaacac
     2941 ttctttttcg gcctataaat caaccgcatc cggcatttat tggcggatgc gatgctgccg
     3001 tatcttatcc accctacaag ccatgaaccg tagaccggat aagcgcagcg catccggcag
     3061 ttatgccgca cgttcatccc gcaccgccaa cacctcgggc aaattcaact caatccagtc
     3121 cgccagtgca gcaacctttt cgctcacctg ctcgcccagc ggcgtgaggc tatattccac
     3181 atgcggcggc accaccggat acgcgatacg gttaagaaaa ccatcctgtt ctaacgcctg
     3241 taacgactgc gcaagcatct tttcactcac cccaccgatt ttgcgccgca ggtcgctaaa
     3301 gcgatgagta ccttcgcgta gcgccaccag aatcaacacc ccccaacggc tggtgacgtg
     3361 tttcaacacc tcgcgcgacg ggcactgttc cgcaaagaga ttaccctctt tcagttgttg
     3421 tgacaggcta acctgactca tttcatactt acctttttgc acgtacttac taaaagtaag
     3481 tttaggtgtt agcgtattta aacacaagac aaaacgatgg agacttccca tgatcgctat
     3541 tactggtgcc actggccaac ttggtcacta tattattgaa tccttgatga aaacggttcc
     3601 tgccagccaa atagtggcta tcgttcgtaa tccggcaaaa gcccaggccc tggcagcaca
     3661 aggcattacc gtgcgtcagg ctgactacag cgatgaagcc gcactgacat ctgcacttca
     3721 gggagtggaa aaactactgc tgatctcttc cagcgaagtg ggtcaacgtg ccccgcagca
     3781 tcgtaatgtt attaatgccg caaaggcggc tggcgtgaaa tttatcgctt ataccagcct
     3841 gctacatgca gatacctccc cgctcggcct cgccgatgag cacatcgaga cggagaaaat
     3901 gctggctgat tctggcatcg tttacaccct gctgcgcaac ggctggtaca gcgaaaacta
     3961 cctcgccagc gccccggcag cactggaaca cggcgtattt atcggtgcgg ctggcgatgg
     4021 caaaatcgcc tcagcaacgc gggcagatta tgcgacagct gcggcacgcg tgattagcga
     4081 agccggtcac gaaggcaagg tttacgaact ggcgggcgat agtgcctgga cgttgacaca
     4141 gttagcggca gagctgacca aacagagcgg caaacaggtt acctatcaaa atctgagcga
     4201 agccgatttc gccgcggcac tgaaaagcgt cggactgccc gacggactgg cggatatgct
     4261 ggcggattct gacgttggcg catcgaaagg tggtctgttt gataacagca aaacgcttag
     4321 caaattgatt ggctgcccaa cgacaacgtt agccgaaagc gtaagccatc tttttaatgt
     4381 taataactag ttaattaaag tggcatcctc ccgcatcctc tctgataatg acgggatgcc
     4441 gggagcaatc atgtctgctt cctgaacgtt tcttctgaca gaccaatgga tgccagtaat
     4501 gattagcggc gtgctgtacg ccctgttagc agggttgatg tgggggctta tttttgtcgg
     4561 gccgttgatc gtgccggaat acccggcgat gttgcagtcg atggggcgtt atctggcgtt
     4621 agggttaatt gcgctaccca ttgcctggct gggacgcgtg cgtctgcgtc agttggcgcg
     4681 tcgggactgg cttaccgcct tgatgctcac tatgatgggc aacctcattt attacttctg
     4741 ccttgccagt gccattcaac gtactggcgc gcctgtttcc acgatgatta tcggcaccct
     4801 gccggtggtg attcctgtct ttgccaatct gctttatagc cagcgcgacg gcaaactcgc
     4861 gtggggaaaa ctcgccccgg cactgatttg tattggcatc ggcctggcga gtgtgaatat
     4921 tgctgagtta aaccacggac tccccgattt tgactgggca cgttatacct ctggcatcgt
     4981 gctagcgtta gtttccgtgg tctgctgggc atggtatgcc ctgcgcaacg cccgctggct
     5041 gcgggaaaat cccgacaaac atccgatgat gtgggcgacg gcgcaggcgc tggtcacgct
     5101 gccggtttct ctcatcggct atctcgtcgc ctgttactgg ctgaatatac aaacgccgga
     5161 cttctcctta ccttttggcc cccgtccgct ggtgtttatt agtctgatgg ttgcgatagc
     5221 cgtgctttgc tcatgggttg gcgcactctg ctggaacgtc gccagccagc gattaccgac
     5281 agtgattctc gggccgctga ttgttttcga aacgctggca ggtttgctgt acaccttttt
     5341 actccgccag caaatgccgc cgctaatgac gctgagcggt atcgcgctgt tagtgattgg
     5401 cgtggtcatc gcggtcagag caaaaccaga aaaaccttta actgaatctg tctcagaaag
     5461 ttgacatgct ggcagtgagt taaataagcc tctgctacgt aagggttata gcttttgcct
     5521 taaaggtgta tttaaaatac atcttatctt attaagaatg aggtatcagc tatggcttat
     5581 cgcgaccaac ctttaggtga actggcgctc tctatccctc gcgcttcggc tctgtttcgt
     5641 aaatatgata tggattactg ctgtggcggt aagcagacgc tggcgcgcgc ggcggcacgt
     5701 aaagaactgg atgttgaggt cattgaagct gaactggcaa agcttgctga acaaccgatt
     5761 gagaaagact ggcgtagcgc cccgctggca gaaatcattg accatatcat cgtgcgctac
     5821 cacgatcgtc accgcgagca gctgccggag ctgatcctgc aagcgactaa agtcgagcgc
     5881 gttcacgccg acaaaccgag cgtgccaaaa gggctgacaa aatacctgac catgctgcat
     5941 gaagagcttt ccagccacat gatgaaagaa gagcagatcc tcttcccgat gatcaaacaa
     6001 ggcatgggca gccaggcaat ggggccaatc agcgtaatgg aaagcgagca cgatgaagcg
     6061 ggcgaactgc tggaagtgat caaacacact accaataacg tcacaccgcc gccagaagcc
     6121 tgcaccacct ggaaagcgat gtataacggc attaatgaac tgattgatga cctgatggat
     6181 cacatcagtc tggaaaacaa tgtactgttc ccacgcgccc tagcgggtga gtgatctaaa
     6241 agctggatgg cattgcgcca tccagcatga taatgcggtt atttccgcag ttcagcagcc
     6301 cgcttcttac caataaacag ccagcccaac cccagcgcga taaaccacag cggggtaacc
     6361 agcagcgcct ggcgagtgtc atcttccagt gtcagcaaca cgaccacgaa cacaaagaac
     6421 gccatacata cccagcacat cagcttgccg agcggcatct tgtagatcga cttttcatgc
     6481 agatgaggac gctgtttgcg gtacaccagg taagagcaaa ggataatcgt ccagacgaac
     6541 ataaacagaa tcgcggaaac ggttgtaatc atcgtgaacg cgccaatcac gctaggattc
     6601 acatacaaca tcaccacgcc gccgagcaga cagatacacg agaaggtcaa ccctttcgcg
     6661 ggtactgcgc gcttagaaag tttagcgaac gctttcggcg caacaccttc ctgcgccaga
     6721 ccaaacagca tacggctggt agagaagaca ccgctgttag cggaagacgc cgcagaggtc
     6781 agcaccacaa agttgatcac gctggcagcc gcaggcaaac ctaccaacac aaacagctca
     6841 acgaacgggc ttttctccgg gactaccgaa ctccacggtg tcacggacat aatcacaatc
     6901 agcgcgaaga cgtagaacat aatgatacgg atcggaatgg agttaatcgc gcgtggcagt
     6961 gatttctccg gatctttggt ttccgcagct gttgtaccta ccagctcaat ccccacgaaa
     7021 gcgaaaaccg ctatctggaa tccggcaaag aagccactta aacctttcgg gaaccagccg
     7081 ccgtcattcc acaaatgcgc gaatgacgct tccacaccgg tcggtgactg aaagtgcatc
     7141 gccaccatga ccaggccgac gacaatcagc gacacgatgg cgacgatttt gatcatcgca
     7201 aaccagaact ccatctcacc gaacattttc acggtggcaa ggttgagcgt cagcagcagc
     7261 actatcaccg ccagcgaggc gacccagtcg gagagatcgg ggaaccagaa ctgagcataa
     7321 gccgtgatcg ccaccacgtc tgccataccg gttacaaccc agcagaacca gtaagtccag
     7381 ccggtgaaat atcccgccca cggccccagt aaatcggaag cgaagtcact aaaagatttg
     7441 tattccagat tcgaaagcag caattccccc attgcccgca tcacgaaaaa gagcataaaa
     7501 ccaatgatca tataaacgaa aatgatcgac ggcccggcaa ggctaatcgt tttgccggac
     7561 cccataaaca acccagtacc aatggcacct ccaatggcaa taagctgaac atgtcggttt
     7621 gtgagattgc gccgtagcga ctgttcagcc ggagcctgat catcggcaac gacttttacc
     7681 tgatctacca tgttttttct tcctgtacct gtctgtgttg ttcaggctct acggccttta
     7741 gtcagtctat gatacgacga atctgtatcg ggactcatcg atattaggta acaatacgcg
     7801 ggatgaatac taagatttag atctaatgtt aattttatgt ttaaagtgag tgtcatatca
     7861 cggtgataac gcgaaacagc tcacaaaaat acacgcttac ggggtctttg caacacaaaa
     7921 tagcgccgtt tggcaaactg aagggtttat tgctgaatgc ctgctcccct ctggttcgag
     7981 gggaacagaa tgcgctgctt agaggatttc cagcagttcg acttcaaaca ccagggtgct
     8041 gaacggaggg atggatgcac ctgcgccgcg ctcgccatat gccagttcct gcgggatagt
     8101 cagttcccat ttagaaccta ccggcatcag agtcagtgct tcaatccagc cagggatcac
     8161 gccattaacc gggaattcag cgggttcacc acgagcaacg gagctgtcaa acacggtgcc
     8221 gtcgatcagt ttaccggtgt aatgaacacg aacgcggtcg gtacgtgccg gaattgcgcc
     8281 ttcaccctgg ttgatcacgc ggaattgcag gccagattcg gtgctattca caccttcttt
     8341 tttggcgttt tcttccaggt atttcacgcc ttcagcagcc atcgcctgga aacgctgacg
     8401 acgaacggca tcggcgcgtt cgtggatttc acgcagcgcg cgatgcacca catcaaccgg
     8461 cacagccgga tgtttgcctt ccagcgcatc ggcaatacct gcaaccagtg cttctggcag
     8521 cagcccttcc aggccagatt cactcagttg ttgccctacc tgcaaaccaa tgccgtagct
     8581 tgcttgcgct tcgatggtgt caaaagttgg ggtggtcatc gtatttcctt tcttagggta
     8641 taaaagtagc gggcagcata tcagccacac ttctttgggt aaaactttgt cgcaggaatg
     8701 atgacaaatc ccggggaaaa agaaacaata gcaatatccc gtatgaatat aataaaggcg
     8761 ggtttaccac ggacatcata gcgttagctt attcataggc tatgattgag gaacaagacg
     8821 tggagcagga ggaaaccatg cccgggcgct ttgaactaaa accaaccctg gagaaagtct
     8881 ggcacgcgcc ggacaatttt cgcttcatgg acccgctgcc gccgatgcat cgtcggggga
     8941 tcattattgc cgccattgta ttggtggtcg gatttctgct tccatctgat gatacgccca
     9001 acgcgtcggt ggtgacgcgt gaagcgcagt tagatattca gtcgcaatca caaccgccga
     9061 cagaagaaca gccgcaaact acccaaacgc aaccattcca gccagatagc ggaattgata
     9121 atcaatggcg ctcttaccgt gtcgaacccg gtaaaacgat ggcgcagctg ttccgcgacc
     9181 acggactgcc cgctaccgat gtctatgcaa tggcgcaggt agaaggcgcg ggtaagccgc
     9241 tgagtaattt gcaaaatggg cagatggtga aaatccgcca gaacgccagc ggcgtggtga
     9301 cgggcttaac cattgatacg gggaataatc aacaggtcct gtttacccgc cagccggatg
     9361 gcagttttat tcgtgcgcgg tagatgaagg gtcaaccaaa agccagctaa ggattgtctc
     9421 tttgatgacc ttacgtctgg cttccagtgc ctggggggaa accatactga tatgataaac
     9481 taggccaagc gtatgctggt tgatcaaata atatccccca agcgcagcaa tattaatatt
     9541 cacctgcaat gggtcgatac ctggcttaaa aatattgtat ttcttccctt catccaataa
     9601 ggattccatt aactgcaagt gagcatggtt aatttctaag agacgctgtg attttgcata
     9661 atgcacacct ttactttgat tttcactatg cacaattttt aaaaaccaag gattagcaat
     9721 ataataatcc catgtaaaat caataatttt ctcaatggct ttttgtggcg gaagatctga
     9781 gaaatcaagg gccttttctt tttgtcgaat atcgttccag gtatattcca gcacttcgat
     9841 aaataaattt tctttattac caaagtggtg ataaaccgtc tgtttactac accctgctgc
     9901 ctttacaata ttatctactc tggccccttc ataaccatat tcggcaaatt cattaaccgc
     9961 acatagcagc aatttttcct tcaggctctg tacgttgctg gtatcaatat acataacgct
    10021 tccccacgac ctcttcgtgg tgactatcat aacctgattt gatttcagct tcagaaaaaa
    10081 gggcaaaaac ttgtactttc atcaggttat caaatgtcct gtgatcgcca tcgcatatca
    10141 accaactaga tggtttatct ttttatcctc cagtcgcaag ctaatcatca ccaaggaaac
    10201 aaaattcaaa aaagagagct attgtatgaa atccactcgt cctgttgccg tgattaccgg
    10261 agccactcgc ggtattggaa agggctgtgc gcaggagctt gctcgtggcg ggtttaacct
    10321 gctgattaac gatcgtcccg atgccgacag cgtagaaaaa ctgcatatca cacaacaaga
    10381 atgtttggcg gaaggcgtgg aagtgatttg cttccctgct gatgtcggtg atctatctct
    10441 gcatgaggag atgctcgacg cggcgcaaaa ccagtggggg cggctggact gtctgctcaa
    10501 taacgcgggg atttcagtga aaaaacgcgg tgacctcctc gacctcgaac cagacagctt
    10561 cgatcaaaac attgccatta acacccgcgc gccgttcttc cttgctcagg cattcagtaa
    10621 gcgcttactg gcacagccga aaccggaagc ggagctgccg caccgctcga ttattttcgt
    10681 cagttccatc aacgccatca tgctggcgat gaatcgcggc gaatacacca ttgccaaaac
    10741 cgccgtttct gccgctgctc gtctgtttgc tgctcgcctt tgtaatgaac aaattggcgt
    10801 ttatgaagtg cgccctggtc tcatcaaaac tgatatgact attccggcaa cggcttatta
    10861 cgacgaactg attgccaaag gtctggtgcc gtggggacgt tggggttatc cggctgatat
    10921 tgcttccacc gtccgcgcga tggcggaagg taaactgatt tacacctgtg gtcaggcggt
    10981 cgcaatcgac ggcggcttaa gtatgccgcg cttctgaggc tgaatcatga gagatctgtt
    11041 acaacgcccg gatttattca gtatcaatac cgcgacactg ggctataaaa cgccgctacc
    11101 cgccattatt gatgcctgcg ccgcacgcgg tattggcgca attgccccct ggcgcaggga
    11161 gttgcagggt gaagatctcc agcaaatcac ccgccagctt gccgccagta acatgagcgt
    11221 ttccggtttg tgccgtagca cttactacac cgcgccgaca ctggcggaac gcaaactggc
    11281 gattgatgat aaccgccggg cgctggacga cgcagcggtg cttaacgccg cctgttatat
    11341 gcaggtggtt ggcggtctgc caacgggcac taaagatcta tatgaagcgc gagaacaagt
    11401 gaaacaaggg attcgccagt tgctgccaca ttcgaaagat gtcggcgtgc caattgcgct
    11461 ggagccactg cacccgatga cagctgccga tcgctcttgc ctgtgcacgt tacgtcaggc
    11521 gctggactgg tgtgatgaac tcgatcccga tggtgagttt ggcctcggcg tggcggtgga
    11581 tgtttatcac gtctggtggg atccggatct cgccagtcag atcctgcgtg ccggaaaacg
    11641 catcctcgcg tttcatgttt ccgactggtt aatgccgacc actgatctgg tcaatgaccg
    11701 tggtatgccg ggagatggcg tcatcaatat tccgtcaatt cgtcggctgg ttgaaaacgc
    11761 cggatttaat ggtgctattg aactggaaat attctcgcct tactggtggc agaaagatat
    11821 taacagc
//
LOCUS       DAZPCS010000113        11793 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZPCS010000113 DAZPCS010000000
VERSION     DAZPCS010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11793
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.113"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            38..778
                     /locus_tag="ACHUQ7_002931"
     CDS             38..778
                     /locus_tag="ACHUQ7_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFR1136243.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVTLSGLVATVKG
                     VKQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            837..1223
                     /locus_tag="ACHUQ7_002932"
     CDS             837..1223
                     /locus_tag="ACHUQ7_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFR1136244.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            1232..1561
                     /locus_tag="ACHUQ7_002933"
     CDS             1232..1561
                     /locus_tag="ACHUQ7_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFR1136245.1"
                     /translation="MQFVMRLAREFRRADWRRMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            1533..4574
                     /locus_tag="ACHUQ7_002934"
     CDS             1533..4574
                     /locus_tag="ACHUQ7_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR1136246.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTMQAASSAATAASAHAQTVEKNARAHERMAREVEQTRLRVDALNQK
                     MREEQAQARALAEAQDKAAAAFYRQIDSVKQAGAGLQELQRIQQQIRQARNSGGVGQQ
                     DYLALISEITAKTRALTQAEEQATRQKAAFIRQLKEQATRQNLSSSELLRARAAQLGV
                     SSAAEVYIRKMERAGKATHSLGLKSAAARRELGVLISQMARGNFGALRGSGITLANRA
                     GWTGALMSPKGMMTGGVIGGLVAAVLGLGKAWHDGRKEGEKFNRQLALTGHYAGVTVG
                     QLWKLSRAISGNGITQHAAAGALAQVVGSGAFHGNDIGMVAKAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNALHFLNATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     KRTSDIDNNLNALGSTLQTLSDWWKQFWDAAMNIGREDSLDAQIDALQEKIQRAKKYP
                     WTNASTQVEYDQQRLNDLQEKKRRKDLQDAKAQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKAIKKNTRATRNDEATRLLLQYS
                     QQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLARKN
                     ELIQALTLLDVKQQELQKQTALNDLRKKTVQLTSQLADKERALREQHNLDIATAGMGD
                     KQRQRYQAQLRIRQEYRQQLQQLENDSRQKGTYGTEDYRRAEEVLKGSLKRQLNENKR
                     YWQELEVAQGDWKNGAMRAFQNFTADADNAAGTAEQMFTVAFSSAGNALATFCTTGKL
                     NFKSFTSSLLSDMARIMAQMAMMQAVKGVGSLFGFTTNADGGVYQSADLSRYSGTVVN
                     RPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADTGGSGMVMFAPQYNIEINND
                     GTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            4574..4903
                     /locus_tag="ACHUQ7_002935"
     CDS             4574..4903
                     /locus_tag="ACHUQ7_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1136247.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPSLKAFLERHGGVRAFQWTPPYDWKLIRVVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            4903..5601
                     /locus_tag="ACHUQ7_002936"
     CDS             4903..5601
                     /locus_tag="ACHUQ7_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFR1136248.1"
                     /translation="MQDIRQETLNECTRAEQSARVELWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGSGFELNGRGCAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            5606..6349
                     /locus_tag="ACHUQ7_002937"
     CDS             5606..6349
                     /locus_tag="ACHUQ7_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFR1136249.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            6313..6888
                     /locus_tag="ACHUQ7_002938"
     CDS             6313..6888
                     /locus_tag="ACHUQ7_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFR1136250.1"
                     /translation="MQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSDGWYQVRIAG
                     RDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVAIWMPGVGIM
                     ASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTCFSSLDNMVAQGNVLPVL
                     YGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            6949..10428
                     /locus_tag="ACHUQ7_002939"
     CDS             6949..10428
                     /locus_tag="ACHUQ7_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1136251.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNVVEVNWIDPDNGHETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGNGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITVVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAVGRASDDAEGYLSFYKGLINKTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     PAFSLTPGGRLTAKNADISGNVNANSGTLNNVTINKNCRVLGKLSANQIEGDLVKTVG
                     KPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCRLI
                     VKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLV
                     VVMKKSTAGISIS"
     gene            10496..11095
                     /locus_tag="ACHUQ7_002940"
     CDS             10496..11095
                     /locus_tag="ACHUQ7_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFR1136252.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHASTNAPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     LSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDNRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            11159..>11793
                     /locus_tag="ACHUQ7_002941"
     CDS             11159..>11793
                     /locus_tag="ACHUQ7_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR1136253.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     MKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAATARDDAQRFAEKARQDATVTAEDRK
                     ATAEDVTSTGANAAAAGQSAQDAAGYARAAEQAKNDIDAALTGTLKMANHLS"
BASE COUNT         2774 a         2795 c         3790 g         2434 t
ORIGIN      
        1 acgtaccaga tcacgtacac gaattaagga ggtggtaatg accacaccaa atccactggc
       61 aaagacgaaa ggtgcgggga cgacgttctg gatgtatacc ggcaacggcg atgcgtttgc
      121 gaaccctttg tcggacactg actggctgcg tcttgcgatg gtgaaggatc tgcaacctgg
      181 cgaaatgacc gctgatgcag aagatgacac ttatctcgat gatgaagatg cagactggaa
      241 aacgacaacc caggggcaga aatccgttgg tgatacttcg gcgacgctgg cctggcgtcc
      301 gggtgacagc gggcagaaaa aactggttca gttgttcgac tccggtgaag tctgcgcgtt
      361 tcgtatcaaa tatcccaacg gcactgttga tgttttccgc ggctggctga gctcactggg
      421 taaaaccatt gcctcaaaag acgtgatgac ccgcactgtg aaaatcagcg gtgtggggcg
      481 tccgtatctg gcagaggaag gcactgaaac cgtgggtgtt accggactga cggtggcacc
      541 ggcatctgtc agtgtcaaag cgggagcaac caccacactg acctttacag taaaacctga
      601 cggggccagt gacaaagcga tcagtgtgca ttcgtcagat cctcagactg cttcggtgac
      661 cctgagcggg cttgtggcca cggtgaaagg cgtgaagcag ggcagtgtca gcatcgtggg
      721 catgacctct gacggagagt ttgtggcagt ggctgcggtg accgtcagcg caccataaca
      781 ggacgatact catcattgcc ccggttatcc ggggcttttt gcatccggag aacatgatgt
      841 ttctgaaaca ggatacgttt aattatgaaa aacagtccgt ggtgctcagt gagctgtccg
      901 ggctgcagag aattgaatat ctgacgtttg ttcagcagcg aacggcaaag tttgatgcac
      961 aggagggaga actgccggag gctgaacgac agattgcttt tctgcgtatg ggaatggata
     1021 tcaatgcctg gctggtttcc cgctcactgt ggaatgctga gcagtctcag gatgttgaga
     1081 cgctttgcgc atccattatg acaacatggt cgtatgatgc gctgggcgcg ggggcggaga
     1141 gggttctgtc gctgagcggt atggggacca ttgagaatgc cggggatgat gatcatgagg
     1201 cgctgacgcc ggaaaagtcc tgacgcggga aatgcagttt gtcatgcggc ttgcccggga
     1261 gttccggcgg gcagactggc ggcggatgct gtcggatatg tcggccactg agcttggtga
     1321 gtggggcgat tatttccgga tgcagagctt cagtgatgtg tggatggatg cgcagtttgc
     1381 ctcgctgaag gcattgatcg tgagaatggt gtccggcagc agcgatgctg cggtggctga
     1441 tttcagcctt ttaccggaag agaacgggat accggagcga acggacgaag aactgatgca
     1501 tcttggggaa ggtatttccg gaggtgtgcg ttatggacca gatagccaac ctggtcattg
     1561 atttggggat tgatgcggca gagtttaaaa atgaaattcc ccgtatcaaa aaccttctga
     1621 atggtgcagc cagcgatgca gaacggtctt ctgcccgtat gcagcgtttt atggagcgtc
     1681 agactcaggc ggcccggcag acaatgcagg cggcttcttc ggctgcaaca gcagcatcag
     1741 cccatgcgca gacggtggag aagaatgcac gggctcatga acgcatggcc cgtgaggtgg
     1801 aacaaacccg tctgcgcgtg gatgccctga atcagaaaat gcgcgaggaa caggcgcagg
     1861 ccagggcact ggcggaggcg caggataaag cggctgccgc cttttatcgc cagattgaca
     1921 gtgtgaaaca ggccggtgcg gggcttcagg aattacagcg tattcagcag cagatccgac
     1981 aggccagaaa cagtggcggg gttggtcagc aggattatct ggcgctgatt tcggagatca
     2041 cggcgaaaac ccgtgccctg acgcaggcag aggaacaggc cacccggcag aaagcggcgt
     2101 ttatccgcca gcttaaagag caggcaaccc gccagaatct gtcgtcttct gagttgcttc
     2161 gcgccagggc ggctcagctg ggggtaagca gtgctgcaga agtgtatatc cgcaaaatgg
     2221 agcgggcagg aaaagccaca cattcgctgg ggctgaaaag tgcggcagcc cggcgggagc
     2281 tgggggtgtt aatcagtcag atggcgcgag gcaattttgg tgcgctgagg ggatccggga
     2341 taacgctggc taaccgtgcc ggatggacag gcgcactgat gtcgccgaaa ggcatgatga
     2401 ctggcggcgt tattggcgga cttgtcgcgg cggtcctggg tctgggtaaa gcctggcatg
     2461 acggccggaa ggagggcgag aaatttaacc gtcagctggc gctgacggga cattatgccg
     2521 gtgtcactgt cgggcagttg tggaaactca gccgggccat atccgggaat ggtatcacgc
     2581 aacatgcggc agccggtgcg ctggcacagg tggtgggcag tggggcattt catggaaacg
     2641 atattggtat ggtggcgaaa gctgccgcac agatggagcg atcggttggc cagtcggtca
     2701 gcgataccat aaatcagttt aagcggctga aggatgatcc tgtaaatgcc gcgaaggcgc
     2761 tggacaatgc gctgcatttt ttgaatgcca ctcagcttga gcagatacgc gtccttgggg
     2821 agcaggggcg gtccagtgat gcggcccgga tcgccatgtc tgcgctggca gaggaaaccg
     2881 gtaaacgcac atctgatatt gataataatc tcaatgcgct gggtagtacg ctgcaaacct
     2941 tgtctgactg gtggaagcag ttctgggatg cggccatgaa tattggtcgt gaagactcgc
     3001 tggatgcgca gattgatgcg ttacaggaaa aaattcagcg cgcgaaaaaa tatccgtgga
     3061 caaacgcctc cacacaggtg gagtacgatc agcagcgtct taacgatctt caggagaaaa
     3121 aacgccggaa ggatttgcag gatgcaaaag cgcaggcaga acggaattac caggagcaac
     3181 agaaacgccg gaatgctgaa aatgccgcgc tgaaccggat gaatgaaacg gaagctgcac
     3241 gacatcagcg ggaaattgcg cgtattaatg ccatgcagta cgccgaccag gctgtcaggg
     3301 atgcggcgat acagcgtgaa aatgaacgtt acgaaaaagc cattaagaaa aatacacggg
     3361 caacccgtaa tgatgaggcc acccggttat tgctgcagta cagtcagcag caggcacagg
     3421 tggaagggca gattgctgct gccagacagt cagcaggcat tgccactgaa aggatgactg
     3481 aagcgcataa acagcttctg gccctgcagc agcgcatcag cgacctggac gggaaaaagc
     3541 tgacggcaga tgaaaagagt gtgctggccc gtaagaatga gctgattcag gcgctgacgc
     3601 tgctggatgt gaaacagcag gagctgcaga aacagacagc gcttaacgac ctgagaaaaa
     3661 aaacggttca gctgaccagc cagctggcag acaaagaacg tgcactgcgt gagcagcaca
     3721 atctggatat tgccactgca ggtatggggg ataagcagcg gcagcgctac caggcacagt
     3781 tgcgcatccg gcaggaatac cggcaacagt tgcaacagct tgagaatgac agtcgccaga
     3841 aaggcactta cgggacggag gactaccgga gggctgagga ggtgctgaag gggagcctga
     3901 agcgacaact gaatgaaaac aaacgctact ggcaggaact ggaagtggcg cagggcgact
     3961 ggaaaaacgg tgccatgcgg gcgtttcaga attttacggc ggatgcggat aatgcggcgg
     4021 gaacggcaga acagatgttt acagtggcat tcagcagtgc cggtaatgca ctggcgacat
     4081 tctgtaccac cggtaagctg aattttaaat ccttcacctc ttccctgttg tcagatatgg
     4141 ccagaattat ggcacagatg gccatgatgc aggcggtaaa gggcgtcggt tctttattcg
     4201 gcttcacgac taatgctgat ggcggtgttt accagtctgc tgatttgagt cgctacagtg
     4261 gcacggtggt taaccgtccg acgttttttg cttttgcaaa aggcgcgggt gtgatggggg
     4321 aagcgggacc tgaagccatt ctgccactgc gtcgcggtgc tgatggtaag ctgggggttg
     4381 tggcggatac tggtggttca ggcatggtga tgtttgcccc gcagtacaac attgagatca
     4441 ataacgacgg cacgaacggg cagataggtc cggctgccct gaaggtggtt tatgacctcg
     4501 ggaaaaaagc ggccgcggac tttatgcaac agcaggcccg tgatggtggc cggttaagtg
     4561 gagcataccg gtaatggaga cgtttcactg gaaagtgcgc ccggatatga atgtggtatc
     4621 agagccgaaa gtggtgacag tgaagctggg cgatggttat gaacagcgtc gtgcggcggg
     4681 actgaataac cagttgtcga cttacagcgt gacgatacgt gttcgtaaag gtgaacaccc
     4741 atctttaaaa gcctttctgg aacggcacgg tggcgtccgc gcatttcagt ggacgccacc
     4801 ttatgactgg aaactgatca gggtggtttg tcgtaaatgg tcggcaagcg tgggggcgtt
     4861 atgggtgact gtaacggccg attttgaaca ggtggtgaac tgatgcagga tatccggcag
     4921 gaaacactga atgaatgcac ccgtgcggag cagtcagccc gggtggagct ctgggaaatc
     4981 gatctgacag aggtcggtgg tgagcgttat tttttctgta atgagcagaa cgaaaaaggt
     5041 gagccggtca cctggcaggg gcggcagtat caggcatacc ccattcaggg gtcggggttt
     5101 gaactgaacg gcaggggctg tgctgcccgt ccgacactga cggtctctaa cctgcacggc
     5161 atggtcaccg ggatggcgga agatctgcag agtctggtcg gcggaacggt ggtccggcgt
     5221 aaggtttacg cccgttttct ggatgcggtg aacttcgtca acggaaacag cgacgccgat
     5281 ccggaacagg aggtgatcag ccgctggcgc atcgagcagt gcagcgaact gagcgcggtc
     5341 agtgcctctt ttgtgctggc cacaccaacg gagacggatg gcgcggtttt cccggggcgt
     5401 atcatgctgg cgaatacctg tatgtggact taccgttctg atgagtgtgg ttacacgggc
     5461 agggcagtgg ctgacgagtt cgacaaacca acaacggata tccggaagga caaatgcagc
     5521 aagtgtatgc gcgggtgtga gttgcgcaac aataccggta atttcggcgg tttcctttcc
     5581 atcaataaac tttctcagta aatccatgac acagacagaa tcagcgattc tggcgcacgc
     5641 ccggcgatgt gcgccagcgg agtcgtgtgg cttcgtggtg agaacgccgg agggagacag
     5701 gtatcttccc agcgagaata tctccggtga gccggaggaa cggttccgga tggctccgga
     5761 ggactggctg cgggcacaaa tgcagggtga gattgtggca ctggtccaca gtcatcccgg
     5821 tggtctgccc tggctgagtg aggctgaccg gcggctgcag gtgcagagtg atttgccgtg
     5881 gtggctggtc tgccggggga cgattcacaa gttccgctgt gtgccgcatc tcaccgggcg
     5941 gcgctttgag cacggggtga cggactgtta cacgctgttc cgggatgctt atcatctggc
     6001 ggggattgag atgccggatt ttcatcgcgg ggatgactgg tggcgtcacg gtcagaatct
     6061 ctatctggat aatctggagg ccacagggct gtatcaggtg ccgttgtcat cagcacaacc
     6121 gggcgatgtg ctgctgtgct gttttggttc atcggtgccg aatcatgccg ccatttactg
     6181 cggcgatggt gagctgctgc accatattcc tgaacaactg agtaaacgag agaggtatac
     6241 cgacaaatgg cagcgacgca cacactccct ctggcgtcac cgggaatggc acgcatctgc
     6301 ctttacgggg atttgcaacg atttggccgc cgcatcgacc ttcgtgtgaa aacgggggct
     6361 gaagccatcc gcgcactggc cacacagctc ccggcgtttc gtcagaaact gagtgacggc
     6421 tggtatcagg tgcgcattgc cgggcgtgat gcaggtgaaa ccgaattgtc tgcccgtctt
     6481 aatgagccgc tggcaaatgg tgcagtgatc cacatagtac cgcgtctggt gggagctaaa
     6541 agtggcggtg tgtttcaggc ggtgctgggg gcagctgtta tggcggttgc tatatggatg
     6601 ccgggggtag gaattatggc gagtaatctg ctgttttctc tcggtgccag tatgacgctt
     6661 ggcggtgttg cacagatgct ggcaccgaaa gccagaactc cccgtacaca gacaacggat
     6721 aacggcaaac agaacacctg tttctcctca ctggataaca tggttgccca gggcaatgtt
     6781 ctgcctgttc tgtacggtga aatgcgcgtg gggtcgcggg tggtatctca ggagatcagc
     6841 acggcagacg aaggggatgg tggtcaggtt gtggtgattg gtcgctgatg caaaatgttt
     6901 catgtgaaac cgcctgcggg cggttttgtc gtttatggag cgtgaggaat gggtaaaggc
     6961 agcagtaagg ggcatacccc gcgcgaagcg aaggacaacc tgaaatccac gcagttgctg
     7021 agtgtgattg atgccatcag cgaagggccg gttgacggtc cggtggatgg attaaaaagc
     7081 gtgctgctga acggtacgcc ggtcctggat agcgagggga agacaaactt ttccggtgtt
     7141 acggtggtgt tccgcgccgg cgagcaggag cagacaccgc cggaggggtt tgaatcttcc
     7201 ggctcagaga ctgtgctggg tacggaagtg aaatacgaca cgccgatcac ccggaccatc
     7261 acgtcggcaa acattgatcg tctgcgcctg accttcggtg tgcaggcact ggtggaaacc
     7321 acctcaaagg gggaccggaa tccgtcggaa gtccgcctgc tggttcagat acagcgtaac
     7381 ggtggctggg tgacggaaaa agacatcacc attaagggca aaaccacttc gcagtatctg
     7441 gcctcggtgg tggtgggtaa cctgccgccg cgcccgttta atatccggat gcgcaggatg
     7501 acaccggaca gcaccacaga ccagctgcag aacaaaacgc tctggtcgtc gtacaccgaa
     7561 atcatcgatg tgaaacagtg ctacccgaac acggcgctgg tcggcgtgca ggtggattca
     7621 gagcagttcg gtaaccagca ggtgagtcgc aattatcatc ttcgcgggcg cattctgcag
     7681 gtgccgtcga actataaccc gcagacgcgg caatacagcg gtatctggga cggaacgctt
     7741 aagccagcat acagcaacaa catggcctgg tgtctgtggg acatgctcac tcatccgcgc
     7801 tacggcatgg ggaaacgtct tggtgcggca gatgtggaca aatgggcgct gtatgtcatc
     7861 ggccagtact gcgaccagtc agtgccggac ggatttggcg gcacggagcc gcgcatcacc
     7921 tgtaacgctt acctgaccac acagcgtaag gcgtgggatg ttctcagtga tttctgctcg
     7981 gcgatgcgct gtatgccggt atggaacggg cagacgctga cgttcgtgca ggaccgaccg
     8041 tcggataaag tgtggaccta taaccgcagt aatgtggtga tgccggatga tggcgcgccg
     8101 ttccgctaca gcttcagcgc cctgaaggac cgccataatg tcgttgaggt gaactggatt
     8161 gatccggaca acggtcatga gacggcgaca gagcttgtgg aggacacgca ggccattgtc
     8221 cgttacggtc gtaacgtcac gaagatggat gcctttggct gtaccagccg gggacaggcg
     8281 caccgcgccg ggctgtggct gattaaaacg gaactgctgg aaacgcagac cgtggacttc
     8341 agcgtcggcg cagaagggct tcgccatgta ccgggtgatg tcattgaaat ctgcgatgat
     8401 gattatgccg gtatcagcac cggtggtcgc gtactggcgg tgaacagcca gacccggacg
     8461 ctgacgctcg accgtgaaat cacgctgccg tcctccggta ccacgctgat aagcctggtt
     8521 gacggaaatg gcaatccggt cagcgtggag gttcagtccg tcaccgacgg cgtgaaggtg
     8581 aaagtgagcc gggttcctga cggcgttgca gaatacagcg tgtgggggct gaagctgccg
     8641 acgctgcgcc agcgcctgtt ccgctgcgtg agtatccgtg agaacgacga cggcacgtat
     8701 gccatcaccg ccgtgcagca tgtgccggaa aaagaggcca tcgtggataa cggggcacac
     8761 tttgacggtg accggcgcgg cacggtgaat ggtgtcacgc cgccagcggt gcagcatctg
     8821 accgccgaag tcaccgcaga cagcggggaa tatcaggtgc tggcgcgatg ggacacgccg
     8881 aaggtggtga agggtgtgag cttcctgctt cgcctgaccg tggcagcgga cgacggcagt
     8941 gagcggctgg tcagcacagc caggacgacg gaaaccacat accgcttcag gcagctggcg
     9001 ctggggaatt acagtctgac agtccgggcg gtaaatgccc gggggcagca gggcgatccg
     9061 gcgtcggtat cgttccggat tgcggcaccg gcagcgcctg tcactattga actgataccg
     9121 gggtattttc agataacggt ggtcccgaaa ctggctgtat atgacccgac ggtgcagttt
     9181 gagttctggt tttcggaaaa gcggattgcg gatatcaggc aggttgaaac cagcgcccgc
     9241 tatcttggca cggcgctgta ctggatagcc gccagtatca atatcaggcc gggccatgat
     9301 tattattttt acgttcgcag tgtgaacacc gttggcaaat cggcatttgt ggaggccgtc
     9361 ggtcgggcga gcgatgatgc ggaaggttat ctgagttttt ataaagggtt gatcaataaa
     9421 acgcatctcg gcaaggagtt gtggacgcag attgataacg gtcagcttgc gccggacctg
     9481 actgaaatca ggacgtccat tacgaatgtc agcaatgaaa tcacgcaaac cgtcaataaa
     9541 aaactggaaa atcagagtgc tgcaatccag cagatacaga aagttcaggt tgatacaaat
     9601 aataacctga acagcatgtg ggctgtgaag ctgcaacaga tgaaggacgg acgcctttat
     9661 attgcgggta tcggtgccgg tattgagaat acgccagcag gaatgcagag tcaggtgctg
     9721 ctggcggcag acaggattgc gatgattaat cctgcgaatg gcaacacaaa gccgatgttt
     9781 gttggtcagg gcgatcagat attcatgaac gacgtgttcc tgaaacgcct gacggctccg
     9841 accattacca gcggcggtaa tcctccggca ttttccctga cacctggcgg acggctgacg
     9901 gcgaaaaatg ccgatatcag cggtaacgtg aacgcgaact ccgggacgct caacaacgtc
     9961 acgattaata agaactgtcg ggttctggga aaattgtccg cgaaccagat tgaaggcgat
    10021 ctcgttaaaa cagtgggcaa acctttccca cgggactccc gggcaccgga gaggtggcca
    10081 tcagggacca ttaccgtcag ggtttatgac gatcagccgt ttgatcggca aattgttatt
    10141 cccgcggtgg cgtttcgcgg tgctaaacat gagcggaaga ataacaatat ttattcgtca
    10201 tgccgcctga tagtgaagaa aaacggtgct gaaatttata accgaacgac cctggataat
    10261 acgctgatat atacgggtgt tattgatatg cctgccggtc acggtcacat gacgctggag
    10321 ttttctgtat cggcatggct ggtaaatggc tggtatccca cagcaagtat cagcgatttg
    10381 ctggttgttg tgatgaagaa atccacagca ggtatcagta tcagctgaat tttataaccc
    10441 agatacgggc accagaaatg gtgccttttt tattgcagaa aagcgagagg taattatgcg
    10501 taaactttat gctgccattt tgtccgcagc catctgtctg gctgtatccg gtgcgcctgc
    10561 atgggcgtct gaacatcagt ccacgctgag cgcggggtat cttcatgcct cgacgaacgc
    10621 tcccggcagt gatgatctga acgggattaa cgtgaaatac cgttatgagt ttacggacac
    10681 gctggggctg gtgacgtcat tcagctatgc caacgctgaa gatgagcaaa aaacgcatta
    10741 cagtgatacc cgctggcatg aggattccgt gcgtaaccgc tggttcagcg tgatggcggg
    10801 gctgtctgtg cgcgtgaatg aatggttcag cgcgtatgcg atggcgggcg tggcttacag
    10861 ccgtgtgtcg accttctccg gggattatct ccgcgtaact gacaacaagg ggaaaacgca
    10921 cgatgtgctg accgggagtg atgacaaccg ccacagcaac acgtctctgg cgtggggggc
    10981 tggcgtgcag tttaacccga ccgaatccgt ggccattgac cttgcttatg aaggttccgg
    11041 cagtggcgac tggcgcactg acggtttcat cgtgggtgtc ggttataagt tctgattagc
    11101 caggtaacac agtgttatga cagcccgctg gttcaggcgg gctttttgtg gggtgaatat
    11161 ggcagtaaag atttcaggtg tactgaaaga tggggcgggt aaacctgtcg taaattgtgc
    11221 gattgaactg cgggccagaa gaaccagtcc gaccgttgtg gcacacgttg ttgccacttg
    11281 cgtgacggac aataacggtg cttatgtgat tgaggctgag ccggggtatt acgaggttgc
    11341 gcttcactgt aacggctggc agccaacccg tgtcggggat attgatgtgg caccgactga
    11401 tgcaccgggg acactgaacg cgtttctgaa tgcaccaaag gatggtgatt tacgtccgga
    11461 ggtgatgaag cgctttgagg aaatggtggc gcaggcgcag cagagtgccg gggctgcagc
    11521 cggaaacgca cagcagacgg cgcaggatgt ggcggcagcc gcaacggccc gtgatgatgc
    11581 acaacgtttt gcggagaaag cccgacagga tgcaaccgtc acagctgagg acagaaaggc
    11641 cactgcggaa gatgtgacaa gcacaggagc aaatgcagcc gcagccggac agagcgcaca
    11701 ggatgccgca ggttatgccc gcgcagcaga acaggccaaa aatgacattg atgctgcgct
    11761 gaccggcact ctgaaaatgg ctaaccatct gtc
//
LOCUS       DAZPCS010000114        11785 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZPCS010000114 DAZPCS010000000
VERSION     DAZPCS010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11785
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.114"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..851
                     /locus_tag="ACHUQ7_002942"
     CDS             <1..851
                     /locus_tag="ACHUQ7_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFR1136254.1"
                     /translation="RHIIIGNEGALCYITEVTVKIFKYTPENNLFYGYILEDMKTGFN
                     ILREIMVEGYRPSIARLYDAEDGTQHFTHFADGKCVLIFMAEGNPRIAKATGEGIAEI
                     VARYPQCQRVDSKLIETWFNNLNWGPDKVAAERVQILKTGNMGFTTEVSGCWSCIHEI
                     YESVINRIRTEFPHADDITMLGGHSSHSYQNGTNMYFVYDYNVVDCKPEEEIDKYHNP
                     LNKIICEETIRLGGSMVHHHGIGKHRVHWSKLEHGSAWALLEGLKKQFDPNGIMNTGT
                     IYPIEK"
     gene            873..2282
                     /locus_tag="ACHUQ7_002943"
     CDS             873..2282
                     /locus_tag="ACHUQ7_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1136255.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     ATLTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLCQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            2260..3039
                     /locus_tag="ACHUQ7_002944"
     CDS             2260..3039
                     /locus_tag="ACHUQ7_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFR1136256.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGSQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            3036..3896
                     /locus_tag="ACHUQ7_002945"
     CDS             3036..3896
                     /locus_tag="ACHUQ7_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFR1136257.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDAMVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSMRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     SRDPLAEARRVLVVGQGGEADNQEIVMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFMVAINHDASAAVFSQADIGVVDDWKVVLEA
                     LVTNIHAEYQ"
     gene            complement(4044..4619)
                     /locus_tag="ACHUQ7_002946"
                     /pseudo
     CDS             complement(4044..4619)
                     /locus_tag="ACHUQ7_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
     gene            complement(4636..4896)
                     /locus_tag="ACHUQ7_002947"
     CDS             complement(4636..4896)
                     /locus_tag="ACHUQ7_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFR1136258.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GDLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            complement(4887..6158)
                     /locus_tag="ACHUQ7_002948"
     CDS             complement(4887..6158)
                     /locus_tag="ACHUQ7_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR1136259.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARADLSVLLLERAEIPGSKNL
                     SGRRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWNVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRTLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGRYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWHHLRRHGLWHLAGDVIRSLRCL"
     gene            complement(6236..6598)
                     /gene="queD"
                     /locus_tag="ACHUQ7_002949"
     CDS             complement(6236..6598)
                     /gene="queD"
                     /locus_tag="ACHUQ7_002949"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFR1136260.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            6917..8716
                     /gene="cysJ"
                     /locus_tag="ACHUQ7_002950"
     CDS             6917..8716
                     /gene="cysJ"
                     /locus_tag="ACHUQ7_002950"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFR1136261.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            8716..10428
                     /gene="cysI"
                     /locus_tag="ACHUQ7_002951"
     CDS             8716..10428
                     /gene="cysI"
                     /locus_tag="ACHUQ7_002951"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFR1136262.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMVKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            10502..11236
                     /gene="cysH"
                     /locus_tag="ACHUQ7_002952"
     CDS             10502..11236
                     /gene="cysH"
                     /locus_tag="ACHUQ7_002952"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFR1136263.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            11500..11652
                     /locus_tag="ACHUQ7_002953"
     CDS             11500..11652
                     /locus_tag="ACHUQ7_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFR1136264.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
BASE COUNT         2836 a         2993 c         3240 g         2716 t
ORIGIN      
        1 ttcgtcacat catcatcggc aacgaaggtg cattgtgcta tatcactgaa gtaacagtga
       61 aaatctttaa atacaccccg gaaaacaacc tcttctacgg ctatatcctg gaagacatga
      121 aaaccggctt caacatcctg cgtgaaatca tggtggaagg gtatcgtccg tcgatcgctc
      181 gtttgtatga cgctgaagat ggcacccaac acttcaccca ttttgccgac ggaaaatgtg
      241 tgctgatctt tatggctgaa ggtaaccctc gcattgcgaa ggcgacgggc gaagggattg
      301 cggaaatcgt tgcccgctac ccgcagtgcc agcgcgtgga cagcaaactg attgaaacct
      361 ggtttaacaa cctgaactgg ggaccggata aagtggctgc cgaacgtgtg cagatcctca
      421 aaaccggcaa catgggcttt accaccgaag tgtccggctg ctggagctgc atccacgaaa
      481 tctacgaaag cgttattaac cgtattcgta ctgagttccc gcacgccgac gacatcacca
      541 tgctgggcgg tcactcctct catagctatc agaacggcac caacatgtac ttcgtctatg
      601 actacaacgt tgttgactgt aagccggaag aggaaatcga caagtaccac aatccgctca
      661 acaaaatcat ctgcgaagaa accattcgcc tcggcggttc gatggtgcac caccatggca
      721 ttggtaaaca tcgcgttcac tggagcaagc tggaacacgg cagcgcgtgg gcgttgctgg
      781 aagggctgaa aaagcagttc gatcctaacg gcattatgaa tacgggtact atctatccga
      841 ttgaaaaata atgtatcagg cagcgttccg cgatgacggg gcgttgcctt ttcggcttct
      901 caggcgagaa gccgttctta ttaccggaca atgaaggggt aaagatgaac acttcaccgg
      961 tgcgaatgga tgatttaccg cttaaccgtt ttcactgtcg aattgctacg ctcactttcg
     1021 gcgcacacct gaccgacggt tatgttctag gcgtcattgg ttacgccatt attcaactta
     1081 cgcccgccat gcaactgacg ccgtttatgg cgggaatgat cggcggctcg gcgctccttg
     1141 gtttgtttct tggcagcctg gttcttgggt ggatctccga ccatattggt cggcaaaaaa
     1201 tcttcacctt cagctttttg ctgattacgc tcgcttcgtt cttgcaattt tttgccacca
     1261 cgccagagca tcttattggg ctgcgcattt tgatcggcat tggtctggga ggcgattact
     1321 cagtaggtca caccttgctg gctgaatttt ccccgcgccg ccatcgcggt attttgctgg
     1381 gcgcattcag cgtggtgtgg accgtaggct atgtgttggc aagtattgcc ggacatcact
     1441 ttatttccga aaacccggag gcctggcgct ggttgctggc atcggcagct ctgcccgcgt
     1501 tgttgattac gttattacgc tggggaacgc cagaatcgcc acgttggcta ctgtgccagg
     1561 ggcgttttgc agaagctcac gctatcgtgc atcgctattt tggtccccat gttttactgg
     1621 gcgatgaagt ggtaacggcg acccataaac acatcaaaac cttgttctct tcgcgttact
     1681 ggcggcgcac ggcgtttaac agcgtcttct ttgtctgcct cgtaatccca tggtttgtga
     1741 tttatacctg gctgccaact atcgcccaga ctattggtct ggaagatgcg ctgactgcca
     1801 gcctgatgct taatgcgttg ttaattgtgg gcgcgctgct gggattagtt ctgacgcacc
     1861 tgctggcaca tcgcaaattt ttgctgggaa gttttttgct gctggcggca acgctggtag
     1921 taatggcctg tttgccttcc ggcagttcat taacgctgct gctttttgtt ctcttcagca
     1981 ccaccatttc ggcagtcagt aatctggtgg gcattttgcc tgcggaaagt tttcctactg
     2041 acattcgctc gctgggagtc ggttttgcca ccgccatgag tcgacttggc gcggcggtaa
     2101 gtactggcct gctgccgtgg gtgctggcgc agtggggaat gcaagtcacc ttattgctcc
     2161 tagcgacagt gttgttggtt ggttttgttg tgacctggct atgggcacca gaaactaaag
     2221 ccctcccgct ggtggcggcg ggaaatgtag gaggtgcgaa tgaacattct gttagcgttt
     2281 aaagccgaac cggatgccgg aatgctggcg gaaaaagagt ggcaggcggc ggctcagggt
     2341 aatagcggac cggatgtttc gctactgcga agtttactcg gtgctgatga acaggccgct
     2401 gccgcgctgc tgttagcgca gagaaaaaac ggtacgccga tgtctttaac cgcgttgagc
     2461 atgggggatg aacgggcgtt gcactggctg cgctatctca tggctcttgg gtttgaggaa
     2521 gctgtattgc tggagacggc ggcagatctg cgctttgcgc cggaatttgt cgctcgccat
     2581 attgccgaat ggcagcgtca gaatccgctg gatctgataa ttaccggcag ccaaagcagc
     2641 gaagggcaga atgggcaaac gccatttttg ctggcggaaa tgctgggctg gccctgcttc
     2701 acccaggtgg agcgtttcac tctcgacgcg ctgtttatca ccctcgaaca gcgtactgaa
     2761 catgggttgc gctgttgccg ggttcgcttg cctgcggtga ttgccgtgcg tcagtgcggt
     2821 gaagtggcct tgccagtacc gggaatgcgc cagcgaatgg ctgcggggaa agcagaaatt
     2881 atccgtaaaa ctgttgctgc cgaaatgcca gcgatgcagt gtctgcaact ggcaagagca
     2941 gagcaacgac gcggagcgac gctgattgat gggcaaacgg tagcagaaaa agctcaaaag
     3001 ctatggcagg actatttacg ccaaaggatg cagccatgaa tatcgcaatt gtgacaataa
     3061 atcaggagaa tgccgcaata gcgagctggc tggcggcgca ggatttcagc ggttgtacgc
     3121 tggcgcactg gcaaatagaa cctcagcccg tagtggcgga acaggtgctg gatgcgatgg
     3181 tggaacagtg gcagcgaacg cctgcggacg ttgttctctt tccgccggga acgtttggcg
     3241 atgaactgtc gacccggctg gcctggcgtt tacatggtgc aagcatctgt caggtgactt
     3301 cgttagatat tccaaccgta agcatgcgca aatcccactg gggaaatgcg ttaacggcga
     3361 cgctgcaaac agaaaaacgc ccgttatgcc tttccctggc gcgtcaggcg ggagcagcga
     3421 aaaacgccac gttgccctct ggaatgcagc aactgaacat tgtcccaggt gcgcttcccg
     3481 actggctggt tagcacagag gatctgaaaa atgttagccg cgacccatta gctgaggcca
     3541 gacgggtgct ggtggtggga cagggcggag aggcagataa ccaggaaatc gttatgctgg
     3601 cagaaaagct gggagccgaa gtgggctaca gtcgggcgcg agtgatgaac ggtggcgttg
     3661 atgctgaaaa agtgattggt atttccggac atctgctggc acctgaggtg tgcattgttg
     3721 ttggcgcttc cggcgcagca gcgttaatgg cgggcgtacg caatagcaag tttatggtgg
     3781 cgattaatca tgacgccagc gcagcggtat tttcacaggc agatattggc gtggttgatg
     3841 actggaaagt ggtgctggag gcactggtga caaacattca cgcagaatac caataacgtg
     3901 ctgccggatg atcctgccta cggttctgga ctggggacct gtaggcctga taagacgcgt
     3961 taagcgtcgc atcaggcgct gaatgtcgga tgtggcgtgc ggccaacaaa tcaaaacaaa
     4021 ttcaattggt tacgattatc ccgtcaaagc aattttttcg ctaacgtcca gactccggta
     4081 ttcgtggtgg aaagcgccac gacacccgcg ttaatcgcat tacgcgcatc ttcttcatcg
     4141 cataccagcc cgccagcaat cagcggttgg cggatttttt ccgtcaccca gcccagcact
     4201 ttgggcatgc agcctggcag gatctcaata caatccggat tcgattgcgc aacttactta
     4261 tcaatgttgt gaaacgaaat cgaatcaaca ataaacaggt gatgaataca gaaaaaaact
     4321 tccgctcttg ccgcttttag catcgatgct ttggtgctga taattccatc cgcctcggtc
     4381 accagtttca aaaactgaat caccacttct ttatttgacg cgccttccag caaatcaaca
     4441 tgaataaaag catatttccc ggcgttctta atctttttaa cgatattact gatggtgcag
     4501 atattgccgt acaacacgga aataaactgg cattcagaat cgatggccag ttgcaggctg
     4561 gcattatctt taacggcagc aatcaccgga ttctggcgga gcaggtgtaa gaggggcata
     4621 aattatcctt attatttatc caaagcggta ggtgatgccg aatccggaag gcggatagcg
     4681 ccactgttgt agtgtggatt cgtcgcacag caaacggcag gtgccacact ccaggcagcc
     4741 gcgatagtca atacgtaagt caccttcctg tgtgagagaa aaaagaccta ccggacaggc
     4801 gttaatcaac cgttgtacca tctgacgctc aacggagtca gccggaacaa tgtgcggcgc
     4861 atcagcaacg cgccagagat tacgggctac agacatcgca gactcctgat aacatcgccc
     4921 gccagatgcc acaggccatg acgacgtaaa tgatgccaga ataactggcg cagcggtgga
     4981 acaggtttat caccctgatc ccataaatcg cgggaaatat cctgcattaa cgcaggccac
     5041 gcccgatacc tgccagggcg ttgcaaaagc gccggaacat gctgataacg ttgtagaaca
     5101 tcccatagca ggctgcgctc tacgttgtga tgataaagcg caaacagatt ttgcggctcg
     5161 cggtgctggc aggcgcttat tagcgtttgt gccgccgcct gtgcgccagt cagcgccata
     5221 tccatgccgc gcacggaaat tccggtattg acgcaactgc gcaacgcatc gcccaccagc
     5281 agccagccgt taccggcgta ttgcaccggc atactgtgca agccaccttc tggcaccaga
     5341 tgcgcaccat actccagtga ttccgtgttt ttgataagcg tgcgcactgc cggatgcgct
     5401 ttaaagcgag tcagcagctc gcttgccgga acacgacttt gcgtaaggga agagagcggg
     5461 caaacaatcc ctaacgagag cgtttgttga ttagtataaa gaaatgcgcc gccgggtaag
     5521 tcatcacaga ttccgccgct gaacagcaac gctgcgcctt cgttattctc cagatgaaaa
     5581 cgttcttcaa tagcggatgt ttccagcgac agcacttctt tgatccccaa cgccatcgct
     5641 tcgccagcag gacgagtcac taacccgtga cgttcagcca ggacgctgtt ggcaccttct
     5701 gccagcacca cataacgggc gcggagaata tcgtcaccac aaataacgcc acagactctg
     5761 ccgttttctt catacagtgc atccaccgtc gctccgggga tgcattcgac accttctttt
     5821 tcggcttcgg caaccagcca cggatcgaac cgtgcacgta atacattcca ggattcaccg
     5881 ccgggctgta agctggaaaa cgtcgttgcg ccatccggcg ttaacaggga aaggctttcg
     5941 tgagtgatgc gtcgttcaag aggcgcggtc agatgaaatt gcgggaggag ttccgcgagt
     6001 gcatgggtat ataaccgcct gccggaaaga tttttgctgc cggggatttc agcgcgttcg
     6061 agtaacaaaa cggataaatc cgctcgcgcg cagcgtaacg cgcaagcggt gcctgcaata
     6121 ccggcaccaa taataataat gtcgcagtcg tcttccatgt catgccccag atatccagaa
     6181 cacaacaccc taacatagcg ttactaaagg gaaattgacc gccgacacac tcttatcatt
     6241 cgccgcgata gatacaccct gcggtgcagg tttcttttac catcaccgca cttaacagcg
     6301 gcacaacggg tttaacctga tcccaaatcc attttgctaa aacttcgctg gttggatttt
     6361 ccagacctgg aatatcattg agataatggt gatcgaggcg ctcataagtt ggcttaaacg
     6421 ccgctttcag ttcagcgaaa tcgataatcc agcccgtatg cggatcgact tccccggtaa
     6481 tttccagtcg caccataaag gaatgcccgt gcaggcgacc acatttatgc ccttccggga
     6541 catgtggtaa gcggtgagcg gcttcgaagg tgaaatcttt aaataacgtg gtggacatca
     6601 taatttctct acaggacgat aaaaccgccg tagagtaccg gaaaggctgt tttttcgcat
     6661 tatctaaaac gactttgttg cgcaaaatcg ctgatttatc ttaatgattg gctaaattca
     6721 tttgtttttc attaggttgg ttaatctatt ttgttgttaa agactattgc taaaacaggt
     6781 tagtcgattt ggttattagt tatcgctatc ccgtctttaa tccacaccgt tcaccccgtt
     6841 aaccttacct tctcttctgt tttatgggcg ctgacagggc gcagaaacag ctttgcttac
     6901 tggaacataa cgacgcatga cgacacaggt cccaccttcc gcgttgcttc cgttgaaccc
     6961 ggagcaactg gcacgccttc aggcggccac gaccgattta acgcctaccc agcttgcctg
     7021 ggtttctggc tatttctggg gcgtgctcaa ccagcagcct gttgcgcttg cagtgacgcc
     7081 agcgcccgcc gcagaaatgc cgggtataac gattatctct gcttcgcaaa ccggcaatgc
     7141 gcgccgggtt gctgaagcat tacgcgatga tttattagca gcaaaactga acgttaagct
     7201 ggtgaacgcg ggcgactata aattcaaaca aatcgccagc gaaaaactgc tcatcgtagt
     7261 gacgtcaacg caaggggaag gggaaccgcc ggaagaagcc gtcgcgctgc ataagttcct
     7321 gttctccaaa aaagcgccaa agctggaaaa caccgcgttt gccgtgttta gcctcggcga
     7381 tagctcttat gaatttttct gccagtccgg gaaagatttt gacagcaagc tggcggaact
     7441 ggggggtgaa cgcctgctcg accgtgtcga ttctgatgtt gaataccagg ctgctgccag
     7501 cgagtggcgc gcccgcgtgg ttgatgcgct taaatcgcgt gcgcctgtcg cggcaccttc
     7561 gcaatccgtc gctactggcg cggtaaatga aatccacacc agcccgtaca gcaaagacgc
     7621 gccgctggtg gctagcctct ctgttaacca gaaaattact gggcgtaact ctgaaaaaga
     7681 cgttcgccat atcgaaattg acttaggcga ctcgggcctg cgttaccagc cgggtgacgc
     7741 gctgggcgtc tggtatcaga acgatccggc actggtgaaa gaacttgtcg aactgctgtg
     7801 gctgacaggc gatgaacctg tcactgtcga gggcaaaacg ttgcctctga acgaagcgct
     7861 acagtggcac ttcgaactga ccgtcaacac cgccaacatt gttgagaatt acgccacgct
     7921 tacccgcagt gaaacactgc tgccgctggt gggcgataaa gcgaagttac agcattacgc
     7981 cgcgacgacg ctgattgttg acatggtgcg tttctccccg gcacagcttg atgccgaagc
     8041 gctaattaat ctgctgcgcc cgctgacccc gcgtctctat tccattgcct cctcgcaggc
     8101 ggaagtcgag aacgaagtac acgtcaccgt tggtgtggtg cgttacgacg tggaaggccg
     8161 cgcccgtgcc ggtggtgcct ccagcttcct cgctgaccgc gtggaagaag agggcgaagt
     8221 ccgcgtattt atcgaacata acgataactt ccgcctgccc gctaacctgg aaaccccggt
     8281 gattatgatt ggcccaggca ccggtattgc gccgttccgc gcctttatgc agcaacgcgc
     8341 cgctgacgaa gcgccaggta aaaactggct gttctttggc aatccgcatt ttacggaaga
     8401 cttcctgtat caggtggagt ggcagcgcta cgtcaaagag ggtgtgctga cgcggatcga
     8461 tctcgcctgg tcgcgcgacc aaaaagaaaa agtttacgta caagataaac tgcgcgaaca
     8521 gggcgcggag ctgtggcgct ggatcaatga cggtgcccac atttatgtct gcggcgacgc
     8581 taatcgcatg gcgaaagacg ttgagcaggc acttctggaa gtgattgccg aatttggtgg
     8641 catggacacc gaagcggcgg atgaattttt aagtgagctg cgcgtagagc gccgttatca
     8701 gcgagatgtc tactaatgag cgaaaaacat ccagggcctt tagtggtcga aggaaaactg
     8761 acagacgccg agcgcatgaa gcttgaaagc aactacctgc gcggcaccat tgcggaagat
     8821 ttaaacgacg gtctgaccgg cggctttaag ggcgataact tcctgctgat ccgcttccac
     8881 ggcatgtatc agcaggatga ccgcgacatc cgcgccgaac gtgctgaaca gaagctggag
     8941 ccgcgccacg cgatgctgtt gcgctgccgt ctgccgggtg gagttatcac caccaaacag
     9001 tggcaggcga tcgataaatt tgccggtgaa aacaccatct atggcagcat tcgcctgacc
     9061 aaccgccaga cgtttcagtt ccacggcatt ctgaaaaaga acgtcaaacc ggtgcaccag
     9121 atgctgcact cggtcggtct tgatgcgctg gcgactgcta acgacatgaa ccgtaacgta
     9181 ctctgcacct cgaaccctta cgagtcgcag ctgcacgcag aagcgtacga gtgggcgaag
     9241 aagatttctg aacatctgct gccgcgtacc cgcgcgtatg cggagatctg gctcgatcag
     9301 gaaaaagtcg ccaccaccga tgaagaaccg atccttggtc agacttacct gccgcgtaag
     9361 ttcaaaacca cggtagtgat cccgccgcag aacgatattg atctgcacgc caacgacatg
     9421 aacttcgtgg cgatcgcgga aaacggcaag ctggtgggct tcaacctgct ggtgggcggt
     9481 gggctttcta tcgaacacgg taacaagaaa acctacgccc gcacggcgag tgagtttggc
     9541 tatctgccgc tagagcatac gctagcggtg gcggaagccg tcgtgacaac tcagcgtgac
     9601 tggggtaacc gaaccgatcg taaaaatgcc aaaaccaaat acacactgga gcgcgtgggg
     9661 gttgagacgt ttaaagcgga agtggagcgt cgcgcgggga tcaaatttga accgatccgc
     9721 ccgtatgaat ttacaggacg cggcgatcgt attggctggg ttaagggcat tgatgataac
     9781 tggcacctga cgctgtttat cgaaaatggt cgcatccttg attatccggg gcgtccgctg
     9841 aaaactggcc tgctggagat cgcgaagatc cacaaaggtg atttccgcat tacggcgaac
     9901 cagaatctga tcatcgccgg tgtgccggaa agcgagaaag cgaagatcga gaaaattgcc
     9961 aaagagagcg ggttaatgaa tgccgtcacg ccgcagcgtg aaaactcgat ggcctgcgtg
    10021 tcattcccga cttgcccgct ggcgatggcg gaagcggagc gtttcctgcc gtcttttatc
    10081 gacaacatcg ataatttaat ggtgaaacat ggtgtcagcg atgagcatat cgtgatgcgt
    10141 gtaacaggct gcccgaacgg ttgtggtcgc gcgatgctgg cggaagtggg cctggtgggt
    10201 aaagcaccgg gtcgctacaa cctgcatctt ggcggcaacc gcattgggac acgtatccca
    10261 cggatgtata aagaaaacat caccgagccg gaaatcctgg cgtcgcttga tgaactgata
    10321 gggcgctggg cgaaagagcg cgaagcgggt gaaggcttcg gcgactttac ggtgcgtgcg
    10381 ggcatcattc gcccggtgct cgatccggcg cgcgatttgt gggattaacc atcagcccgg
    10441 tcttgtaggc ctgataagac gcgtgagcgt cgcatcaggc aaggcaaaca gtgaggaatc
    10501 tatgtccaaa ctcgatctaa acgccctgaa cgaactgccg aaggtagatc gcattctggc
    10561 gctggcagaa actaacgccg aactggaaaa actggacgct gaaggccgcg ttgcctgggc
    10621 gctggataat ctgcccggtg aatatgtact ttcttccagc tttggcattc aggcggcggt
    10681 gagcctgcat ctggtgaatc agattcaccc ggatattccg gtgatcctca ccgacaccgg
    10741 ctatctgttc ccggaaacct accgctttat tgacgagtta acggacaaac tcaagctcaa
    10801 cctgaaagtg taccgcgcta ccgaaagcgc cgcctggcag gaagcacgct acggcaaact
    10861 gtgggaacag ggcgttgaag gcattgaaaa gtacaatgac atcaacaaag tcgaaccgat
    10921 gaaccgggcg ctgaaagaac tgaatgcgca aacgtggttt gctggcctgc ggcgtgaaca
    10981 atccggcagc cgtgccaatt taccggtgct ggcaattcag cgtggcgtat ttaaagtgct
    11041 gccgattatc gactgggata accgaactat ttatcagtac ctgcaaaaac atggcctgaa
    11101 atatcaccca ttatgggacg aaggatattt atcggtgggc gatacccata caacccgtaa
    11161 atgggaaccc ggcatggcgg aagaagaaac gcgtttcttt ggcttaaaaa gggaatgtgg
    11221 attacacgaa gggtaaatat tgccggataa attaaacgcc tctgtcagaa atgatggggg
    11281 cgtttttatt ttgcgagcag cattccggca tttaaaggtg aacatcgcag ggaaaattaa
    11341 caggtataga atggcgttgg gtgcttgagg ctgtctggcg ttaagcattc gcgaggttcc
    11401 agatggacaa aagccccagg cgatatttct atcaacctga ggctcgcgtt cgaacgtagc
    11461 aattcggatg ttagtctctt ctttgcaggg aggcaagaca tgctgacaaa atatgccctt
    11521 gtggcaatca tcgtactgtg ttgtacagta ctgggattca cgttgatggt aggtgactcg
    11581 ttatgtgagt tgagtatcag agaacgtggt atggagttta aggcagttct cgcttacgaa
    11641 tcgaagaagt agccaccacg cgggggtaaa accccgcgta ctgaattgtg atggtttggt
    11701 ctcctgcacc ctttttttaa atttatattt tttatgtgaa tatatagtct ctatttatta
    11761 ttatgaattt caaatgaaaa tcatt
//
LOCUS       DAZPCS010000115        11682 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZPCS010000115 DAZPCS010000000
VERSION     DAZPCS010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11682
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.115"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            47..610
                     /locus_tag="ACHUQ7_002954"
                     /pseudo
     CDS             47..610
                     /locus_tag="ACHUQ7_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
     gene            complement(681..1130)
                     /gene="rplI"
                     /locus_tag="ACHUQ7_002955"
     CDS             complement(681..1130)
                     /gene="rplI"
                     /locus_tag="ACHUQ7_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFR1136265.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(1172..1399)
                     /gene="rpsR"
                     /locus_tag="ACHUQ7_002956"
     CDS             complement(1172..1399)
                     /gene="rpsR"
                     /locus_tag="ACHUQ7_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFR1136266.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            complement(1404..1718)
                     /gene="priB"
                     /locus_tag="ACHUQ7_002957"
     CDS             complement(1404..1718)
                     /gene="priB"
                     /locus_tag="ACHUQ7_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFR1136267.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            complement(1725..2120)
                     /gene="rpsF"
                     /locus_tag="ACHUQ7_002958"
     CDS             complement(1725..2120)
                     /gene="rpsF"
                     /locus_tag="ACHUQ7_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFR1136268.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            2446..2721
                     /gene="yjfY"
                     /locus_tag="ACHUQ7_002959"
     CDS             2446..2721
                     /gene="yjfY"
                     /locus_tag="ACHUQ7_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFR1136269.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            complement(2850..3536)
                     /gene="ulaF"
                     /locus_tag="ACHUQ7_002960"
     CDS             complement(2850..3536)
                     /gene="ulaF"
                     /locus_tag="ACHUQ7_002960"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFR1136270.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(3536..4390)
                     /gene="ulaE"
                     /locus_tag="ACHUQ7_002961"
     CDS             complement(3536..4390)
                     /gene="ulaE"
                     /locus_tag="ACHUQ7_002961"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFR1136271.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETN
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKACDWVKARMA
                     KAGMVEAA"
     gene            complement(4400..5050)
                     /gene="ulaD"
                     /locus_tag="ACHUQ7_002962"
     CDS             complement(4400..5050)
                     /gene="ulaD"
                     /locus_tag="ACHUQ7_002962"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFR1136272.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            complement(5064..5528)
                     /gene="ulaC"
                     /locus_tag="ACHUQ7_002963"
     CDS             complement(5064..5528)
                     /gene="ulaC"
                     /locus_tag="ACHUQ7_002963"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFR1136273.1"
                     /translation="MKLHDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            complement(5538..5843)
                     /gene="ulaB"
                     /locus_tag="ACHUQ7_002964"
     CDS             complement(5538..5843)
                     /gene="ulaB"
                     /locus_tag="ACHUQ7_002964"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFR1136274.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            complement(5859..7256)
                     /gene="ulaA"
                     /locus_tag="ACHUQ7_002965"
     CDS             complement(5859..7256)
                     /gene="ulaA"
                     /locus_tag="ACHUQ7_002965"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFR1136275.1"
                     /translation="MEILYNIFTVFFNQVMTNALLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASLIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWVVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            7611..8675
                     /gene="ulaG"
                     /locus_tag="ACHUQ7_002966"
     CDS             7611..8675
                     /gene="ulaG"
                     /locus_tag="ACHUQ7_002966"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFR1136276.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEIKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            8783..9538
                     /gene="ulaR"
                     /locus_tag="ACHUQ7_002967"
     CDS             8783..9538
                     /gene="ulaR"
                     /locus_tag="ACHUQ7_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFR1136277.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(9535..10284)
                     /gene="yjfP"
                     /locus_tag="ACHUQ7_002968"
     CDS             complement(9535..10284)
                     /gene="yjfP"
                     /locus_tag="ACHUQ7_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR1136278.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            10466..10795
                     /gene="bsmA"
                     /locus_tag="ACHUQ7_002969"
     CDS             10466..10795
                     /gene="bsmA"
                     /locus_tag="ACHUQ7_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFR1136279.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            10944..11219
                     /gene="yjfN"
                     /locus_tag="ACHUQ7_002970"
     CDS             10944..11219
                     /gene="yjfN"
                     /locus_tag="ACHUQ7_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFR1136280.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(11336..>11682)
                     /locus_tag="ACHUQ7_002971"
     CDS             complement(11336..>11682)
                     /locus_tag="ACHUQ7_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="catalyzes the formation of 3-methylcrotonyl-CoA
                     from isovaleryl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFR1136281.1"
                     /translation="GNIMCLDVLRVLNKQAGVYDLLSEAFVEVKGQDRYFDRAVRRLQ
                     QQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQAWCQVMLDTRGGVRLSEQIQN
                     DLLLRATGGVCV"
BASE COUNT         2959 a         3087 c         2870 g         2766 t
ORIGIN      
        1 ccatcttaaa tgactctgag gtgtctgtta aacccgtggc gattcagtaa caatgaatgg
       61 tgataattct tttggatgga accatgaagc tacattagat aatattcacg cccagaaata
      121 caataaagct tacgcgggtg cgaggttaac cgcagaactg aaatatttat tgcaagatgt
      181 cgaatccttc gaacccaaca gtaaatacac aatacacgag gttgttttgg gacctggtta
      241 tagtactcca gactataccg gccaaacgat aggatacgtt gtaacccttc ctgcccaaat
      301 gcctaattgc tgctcatctg aactaccaac aatcgatctt tatatcgacc aacttcacac
      361 tgttacaggc gtttctaatg ccctcggatt catcattgct gcattgctaa acgcgtattc
      421 tgatttaccc catgatttga aaattggtct acgttcctta tcaaattcag ccgcaatcta
      481 ctcgggttta ggtttcgagc gagtgccgca ggaccgagac ataaggagtg atcgtatgca
      541 tctaacgcca gcaaaccatc ctgacttatg gacacaagag aatggtgaat ggatctactt
      601 gcgaaattaa tgcctgacga aaaatagaaa gcaaaacgcc gaccaatggt cggcgttttt
      661 acgtctcgtt gaataacgaa ttattcagct actacgttta cgatcacttt cgcgaatact
      721 tcgctgtgaa cctggaagct cacttcgtgt tcgccagtgg tacgcagaac gccgttcggc
      781 agacgaactt cgctcttagc cacttcaacg ccagctgcag ttacagcgtc agcgatgtcg
      841 cgagtaccga tggaaccgaa cagtttacct tcgtcgccag ctttagacgc gatggtaaca
      901 gtttccagtg cattgatttt ctcagcgcga gcattagcag ctgccagaac ttcagccagt
      961 ttagcttcca gttcagcgcg acgtgcttcg aagaattcaa tgtttttctt ggtagctgga
     1021 acagctttac cctgcggtac caggaagtta cgagcatagc ccgctttaac gtttacctga
     1081 tcacccaggc tacccaggtt tgctacttta tcaagcagaa taacttgcat taccttatcc
     1141 tctcaaagtc gtattaatgg accgtgaccg attactgatg gcgatcagtg tacggcagca
     1201 gggacaggta gcgagcgcgt ttgatagcgc gagccagctg acgctggtat tttgcacggg
     1261 taccggtgat acggcttggg acaatcttac cgctttcggt gatgtagttt ttcagcgtag
     1321 cgatatcttt atagtcgatc tcttgaacgc cttccgcggt gaaacggcag aacttgcgac
     1381 gacggaaata acgtgccata tggctagtct ccagaatcta tcaattcaat ctgctcggca
     1441 tgcaaaacca ttttgctcag tccgttcttt gccttgtggc atgaaatgaa cccctgaacg
     1501 gttatgcgac tgccgaccgt tatactgtga gtaatggcct ggttttcgtg tccgctaaca
     1561 ataacgggca tttgacacca tgcctgccgg tgaaagccgg cttcctcctg cacagaacga
     1621 tgctcaagca cgaactggca gtgaggaatt cctgatggac tgacctttcg aaggggagtc
     1681 ctgcacacgg tgccggacaa caccagacgg ttggtcatca gaaattactc ttcagaatcc
     1741 ccagcttcag catcatcagc ggtttcgttt gcgaaatcat cgcgacgctc acggcgctcg
     1801 tctttcgctt taaccatcgg agatgcttcg gtaacagcgt gcttggtacg cataaccatg
     1861 ctgcggataa cggcatcgtt gaagcggaag gtagtttcca gctcatcgat cacttcctgc
     1921 ggagcttcaa cgttcatcag aacgtagtgt gctttgtgca gtttgttgat cgggtaagcc
     1981 agctgacggc ggccccagtc ttccagacgg tggatcttgc cttctgcacc agtgatggca
     2041 gcagtgtagc gctcgatcat gcccggaacc tgttcgctct ggtcaggatg gaccataaaa
     2101 acgatttcgt aatgacgcat cgaattgctc cttacggatt attcagcctc ctgtctgggt
     2161 cagccgaatc ccggggaggc aaggaacgtg ttaaaggtcg gctgaaaaat gacgcgttat
     2221 agtactggca taccccgcta aactcaaggg cggagcagaa ataaatctcc cctttcgcaa
     2281 ataacctcta aaccgctgat ttgaagttca tcacattccg tcacagtgtt ttttgaacaa
     2341 ctgcatcaga aatggtcacc atcaacttct ttatcattga ttaaaattaa atcagaagat
     2401 catcacagag gagagcacac aaagtcagcc gtcgaggagg tatctatgtt cagtcgagtt
     2461 ttagcccttc tggctgtgct tttgctaagt gcaaatacat gggcagccat tgaaattaat
     2521 aaccaccagg ccagaaatat ggacgatgtg caaagcttag gcgtgattta tatcaatcat
     2581 aatttcgcca ctgaaagcga agcacgtcag gcattaaatg aagaaacaga tgcgcaaggc
     2641 gcaacgtact accacgtaat tctgatgcgg gaaccgggga gtaacggcaa tatgcacgcc
     2701 agcgcggata tttatcgcta gcaccaggta taaccaacga aacattgcca tagtttgctt
     2761 tgcccccttc gcagggggct ttttttgaaa aacggctgcg gaattagacc agttatctcc
     2821 cgaggaagga aatttccgca gcgcgtgttt tacttctgcc cgtaataagc gttaggaccg
     2881 tgcttacgca tgaagtgttt attcatcagg tagctgtcga tatgattgag ttgtggatta
     2941 atactgcgcg caatccacgc cattttcgcc acttcttcca tcactaccgc gttatgcacc
     3001 gcgtcgtggg catctttccc ccaggcgaac ggtccgtgct gatacaccac aattcccggc
     3061 gtatgcagcg gctcggcgtt gcccagcgtt tcgataatca ctttgccagt gttcagttca
     3121 tactcaccct gcacctcttc ttcgcttaat ccacgcgtac acgggatatc accaaagaag
     3181 tagtcagcgt gcgtggtgcc taatgccggg atcgccagcc ccgcctgcgc ccatgcggtg
     3241 gcatgagtgg agtgggtatg gacaatgccg ccaagcgacg ggtaacgacg gtagagttcg
     3301 agatgcgtcg cagtgtcgga agatgggcga tactcccctt ccaccacctt gccgctcata
     3361 tcgaccacca ccatatcgtc tgctttcatg gtttcgtagg caacgccgct gggcttgatc
     3421 accaccagcc cgcgttcgcg gtcgatagcg ctgacgttgc cccaagtaaa ggtcaccagc
     3481 ccatagcgcg gcagatccat gttggcttca aacacctgct gttttagctt ttgcattatg
     3541 ccgcctccac catgcctgct ttggccatgc gcgctttcac ccaatcacac gctttcacca
     3601 cttctgccgc cgggtcttcc gccgtttcgc tccacatctc aatcaggtac ggcccgcaat
     3661 agccactctg tttgagcgtt tcgaagcaac gttcgaaatc caccacacct tcgccaaatg
     3721 gcacgttttt gaagacgccc ggtttggtgt ctttcacatg caccgcgacg atatgcccga
     3781 ttccggcctg caactccatc tgcacatcgt tgtcccacgc cgacaggttg ccgatatccg
     3841 ggtagagctg gaaccacggg ttgttgagat agtgcgcgta acccagcgcc ttgctgatgg
     3901 agttcatcaa cggataatcc atgatctcca tcgccagcgt cacctgcgcg cggctcgcca
     3961 tctcaacgct ctctttcagg ccgtcacgga aacgacgacg cgtttcgtta ttggcttcct
     4021 gatagtaaac gtcatagccc gccagctgga tcacgcgaat gccaacgtcc tgggcgaact
     4081 ggatggcttt acgcataatc tccagcccct gcgcccgcac cgcgtcatct tcgctgccca
     4141 acgggaaacg acgatgggca gaaaggcaca tggacggcac acgcacgccg gtttcaacaa
     4201 tagcattcac cagcgccaga cgctgttcgc ggctccagtc aaggcgcgac aggcgctcgt
     4261 tagtttcatc taccgacatc tcgacaaaat cgaagcccaa cgtttttgcc agtcgcaggc
     4321 gttccagcca gcactccccg gcggggagcg ctttttcata gatgccaagc gggatttgtt
     4381 tggacaacat atccgctcct tagccccaca gttcggcgat ggaacgtttg aactgacgcg
     4441 cggcttcaac cggagaagcg gcatcacgga tgctgcgacc ggcgataaac acatgaattg
     4501 gaatgccttt gaacagcggc agatcttcca gcgccaggcc accggtgacg gtgactttga
     4561 agcccatatc ggaaagacgt ttgatcgcag tgatatcggc ttcaccccac gccacgccag
     4621 cggcctgcgc gtcacggctg cggtgataaa ccacctgctg aatacctgcg tcgcgccact
     4681 gttgcgcctg ttcccaggtc cagtaaccgg tcagttcgat ctgcacgtcg ccgttaaact
     4741 cttttgctac gtccagcgca cctttggcgg tgttgatatc cgcacaacaa atcacggtca
     4801 cccagtcagc gttggcttcg aagcacatac gagaaagaat tttgcccgca tcggcaattt
     4861 tggcgtctgc cagcacgatt ttgtgcgggt agagcgcttt cagatcgcga accgcacgca
     4921 cgccttcgcc cacgcacaga atggtaccca cttcgataat gtcgacttct tcggcaatca
     4981 ggcgagtggt ttcgtaggcg ctatccatag tctggttgtc cagcgcgact tgcaacatcg
     5041 gtaatgacat cttcaattcc ttcttaagct gccgcgttgg tgcggtcaat gagatccagt
     5101 acttcctgct cggtacggca ggcacgtaaa cggtcgaaat tttcttcatc ttcaaacagg
     5161 ttgacgatct gcatgatgcc cacttcctgg tgcgtgttgg catcgaccgc cgccatggtg
     5221 atgaggatat ccaccgggtc gttatcttcg tggttgaact ccagcggctt tttcagcgtc
     5281 accagcgcaa aaccggtttt cttaacgccc tcttccgggc gaccgtgcgg cattgccagg
     5341 cctggtgcga gcacaaagta cggaccgtgc tgttcaaccg catccagaat cgcctggtag
     5401 taacgcggct cgacaacatc cgccgccacc agcaggtcaa caccgatttt tactgcgtcc
     5461 tgccatgtct cagcttcagc ctgcaggcgg atggatttat tctccgccag cgaatcatgt
     5521 aatttcatgg cgcgtcctta cttcacatcc tgcgggaaat gcgctttgat cacttccagc
     5581 agtttcgggc caaagtcggc aggagagagc atgttgcgca cgccaaccac gtatttgttg
     5641 ccggtcacgg tgatttcgcc cgcaatgtgc gtagaagcga tgatgatatc cgcgccgctc
     5701 aactcgcttt tgtattcgcc aaccgcgcag ctatttaccg tgtggtcaat gtttgattgg
     5761 gttaaaaact ggtccacttt catcttcatg atcatggaac tgccttgtcc gttgccacac
     5821 acagccagaa tacgtacggt cataatcaaa actccttatt aagcagactg ttctgccaat
     5881 tgtttttctg catcttcttc tgcgcgcagt gcgcggccag cgaagaacat ataagccagt
     5941 gcaattacaa tgatgacggc cataaaggcg ataccgatgg agaagaagcc ctgcatcatc
     6001 ggcggtgcca gaatcgacca gtccgccatc cccatccagg cactcatacc ggtaagtttc
     6061 accacccaga cgcaaccaaa gatttcgatc atccccatca ccagacaaat cttcagcgcc
     6121 gcacgccagc cgccgaagtg gttagcgaac acgccgatgg tggcgttgga gaagaacatc
     6181 gggataaagc ctgggataat caggatcgag gaaccgcagg cgaccaggat gccaaccgca
     6241 atcagctgac cgatggtgcc ccacataaag ccccagacca cggcgttcgg agcgaagcta
     6301 taaatagccg cacagtcaat cgccagaacc gcaccaggga tcaggcgctg ggagataccg
     6361 ttaaacgctt cagagagttc cgccacaaac atgcgcacac cctgagtgat gatgaagatc
     6421 gccaccgcga aggaaaaacc ggtttgcagg atatagaccg tccagtgcac tttgcctgcc
     6481 atcgcctgca cggtgtcgat accgaaggag agcagaatgg caccaaagaa gatggtcatg
     6541 acaatcgccg tggagacgat gttgtcgtgg aaaatattca gccagcctgg cagtttgagg
     6601 tcttcaacgc tttcctcttt tttgccgagg aacggcgcaa ctttataggc aattaatgat
     6661 gcaaactgtt gctggtgacc gatggagaaa ccgcagccgt ccgtcacttc ctgagtcggc
     6721 ttgtacatca tgttggaggt aatgccccag tagagcgaaa ccagaatcgc cgtgcagata
     6781 atggttgtcc acatggagta gccgaaaata aacaacgtaa cggcaatcag ccccgcctgc
     6841 tggaacatga tatgaccagt cagcatgatg gtacgaatac cggtaatgcg acgcagcagc
     6901 acgtaacaga tgttcagcgc cagcgctaac aacaccgcgt aacctaccca gctataggca
     6961 tcgcccatgc ggtcgatggt tgccatcatc gacgcgtaag tatcggaaat tgcgccgtta
     7021 atgccgtaga cttcggacat tttcgccacc accggtttga aggtgctggt gaggatgccg
     7081 gaccctgcct gcaacaacat gaaaccaatt atggttttaa tcgtgccttt aataataacg
     7141 ctgacacttt tgcgcagtag gatgtagccc agacaggtca caatacccag caacaacagg
     7201 gcattggtca tgacctggtt aaaaaacacg gtaaagatgt tgtagaggat ctccataacg
     7261 atctcctgaa gaagaggtaa ccgggtattc cgcacacccg gaatgttgtg cactcactct
     7321 aattttcaaa agtaatcaca acaagattat ttatgattaa atgtgacgcg acccgcaaat
     7381 aattccacat ggatagagtg ggattcacac aacgaaacaa ttatttattt aaattcattt
     7441 agttaattta actacacatt ccattaaatc tttccccact caaaatcaac aacaacttcc
     7501 ctggcaaaaa gtcaaaaaat ggtcattacc ttcctgaagg aatagtgtca ggattaatca
     7561 aaaccaatca catgttgatt cgaattgaac tatcatgaag gtaaatggcg atgagtaaag
     7621 tgaaaagtat cacccgagaa tcctggatcc tgagcacttt cccggagtgg ggtagctggc
     7681 tgaatgaaga aattgaacaa gagcaggtcg cccctggcac atttgcgatg tggtggctag
     7741 gctgcaccgg gatctggttg aaatcagaag gtggcaccaa cgtttgcgtt gatttctggt
     7801 gcggcactgg caaacaaagt cacggtaacc cgttaatgaa acagggtcac cagatgcagc
     7861 gcatggctgg cgtgaaaaaa ctgcagccaa acctacgcac caccccgttt gttcttgatc
     7921 cgtttgcgat tcgccagatc gacgcggtac tggcgactca cgatcacaac gatcatatcg
     7981 acgttaacgt cgctgctgcc gtgatgcaga actgtgctga tgacgtacca ttcattggac
     8041 cgaaaacctg cgttgatttg tggattggct ggggcgtacc gaaagagcgt tgcatcgtgg
     8101 tcaaaccggg cgatgttgta aaagtgaaag acattgaaat tcatgcgctt gacgctttcg
     8161 accgtactgc actgatcacc ctgcctgccg atcaaaaagc ggctggcgta ctgccagatg
     8221 gcatggacga tcgcgcagta aactacctgt tcaaaacccc tggcggttcc ctatatcaca
     8281 gtggcgattc ccactactct aactattatg cgaagcacgg taacgaacat cagatcgatg
     8341 tggcgttagg atcgtatggt gagaacccgc gcggtattac cgacaaaatg accagcgccg
     8401 atatgctgcg tatgggtgaa gccctgaatg cgaaagtggt tatcccgttc caccacgaca
     8461 tctggtcaaa cttccaggcc gatccgcaag agatccgcgt gctgtgggag atcaaaaaag
     8521 atcgtctgaa gtatggcttc aagccgttta tctggcaggt tggcggcaaa tttacctggc
     8581 cgctggataa agacaacttc gagtaccact atccgcgcgg tttcgatgat tgcttcacta
     8641 ttgaaccgga tctgccgttc aagtcattcc tgtaatctgt cgtttatacc ggatagcgtg
     8701 gagaagttcg cgctatccgg cgtagtattt cctggctatt tcaaatatca tctaaaaaaa
     8761 tcaaatttta tcggaatagc tcatgactga agcacaaaga catcaaatcc tcctggaaat
     8821 gctcgcacaa ttgggctttg tgaccgttga gaaagtcgtt gagcgtctgg gaatttcgcc
     8881 tgccactgcg cgacgcgata tcaataaact tggcgaaagc ggcaaactga aaaaggtgcg
     8941 caatggcgca gaagctatta cccaacagcg cccgcgctgg acgccgatga atctgcatca
     9001 ggcgcagaat cacgatgaaa aagtacgtat tgctaaagcg gcctcgcagc tggttaatcc
     9061 gggcgaaagc gtagtcatca actgcggctc caccgcgttt ctgcttgggc gggaaatgtg
     9121 tggcaagcca gtgcaaatca tcactaatta tctaccgctg gcaaattacc tgatcgatca
     9181 agaacatgac agcgtgatca ttatgggtgg acagtacaac aaaagtcagt ccatcacttt
     9241 aagcccgcag ggcagcgaaa acagtctcta tgccgggcac tggatgttta ccagcggaaa
     9301 agggctgacc gcagaagggt tgtataaaac cgatatgctg acagcaatgg cagagcagaa
     9361 gatgctgagc gtggtaggga aactggtggt actggttgat agcagtaaga ttggcgaacg
     9421 cgcgggaatg ctttttagcc gtgccgatca aatcgatatg ctgatcaccg gcaaaaatgc
     9481 taacccggaa atcctgcaac aactggaagc gcaaggggtc agcattctgc gtgtttaaag
     9541 atgctggcgg aaaaatgtca cggcagcatc taacgcctca ggcgtaatgc ggtgacgcac
     9601 gcctggctgc catgaacagg ttagcagttt atcccgtccc gtctcgctta aggcctgctg
     9661 caaacgtagt gattcgtcgg caggcacaac gtcatcgtcg aggccatgcc acagcagtag
     9721 aggtctgtcc ccaagttgtt ccaggtggtt tgtcgcttcc cactctgcca gcggcgcgac
     9781 aattttattg aattcattct gctgtgctgc cgtttcaggt atcagcggtg gaaacagtga
     9841 acgggcgagc gatgtaaaat agcccgatcc catcatgctg gcggtacatt tcaccgtggg
     9901 gtggcgagcg gtaattccca gtgccgtcat cgcgcccatc gacgcgccac cgactgccag
     9961 acggtcatca agcagccagt tttcttcggc tattgccgca cgtaaagtag tgaattcctg
    10021 catactttgt agcaagattt gccagaattg atttaaccgc cgcgcttcgt cgccactaaa
    10081 acggctaccg tgatcgggcg catccggcat gatcacccgc aaaccagcct gcgccagcgc
    10141 aacggcaaaa tagctataca ccaggctgga tgaagtaaag ccgtgataaa aaattacgca
    10201 cggtaacggg gtatcttttt gcccgacagg ataagcatga agaacgggaa tatctgccag
    10261 ctcgcgtgat tctatttcaa tcatctgtct ctcctttttg tctggtatga catgaagaaa
    10321 tccttagcac aagtttctgc tggcggtatt agttcataat gttgagatgt gggttacgct
    10381 ttcgtcacgt tttcattcga aaatcgcgta ccaggtaaca attcgggaac attcccccaa
    10441 agttaaatta ataaggcact acactatggt tagcaggaaa cgtaatagcg ttatttaccg
    10501 gtttgccagt ttattattgg tgttgatgtt aagtgcctgt agcgcactgc aaggtacgcc
    10561 acagccagca ccgccagtaa cggaccatcc gcaagagatt cgccgcgacc agacgcaagg
    10621 gttacagcga ataggtagcg taagcacgat ggttcggggt tctccggatg acgcattagc
    10681 agaaatcaga gcaaaagctg tcgctgcaaa agctgattat tacgttgtcg tcatggtgga
    10741 cgaaaccatc gtgacaggac agtggtattc acaagccatt ttgtaccgta aataaatcag
    10801 acaacttaca tagtcagctt tacattgctt tgcgtcccta tgcgtttacc tgtgcacaat
    10861 aaattaccgg ctggaagctt gggcgtttat taccgcgatg gaatgccctg caacatgtgg
    10921 ggaaacgaaa aatggagctg acgatgaaac aattacttgc ctcaccctcg ctgcaattag
    10981 tgacttatcc tgcgagcgcc acggcacagt ctgcagaatt cgccagtgct gactgcgtga
    11041 cgggcctgaa tgaaataggt cagatctcag ttagcaatat ttcaggggat ccgcaggacg
    11101 tggaacgtat cgtggcgtta aaggccgatg aacaaggtgc gtcatggtac cgcattatta
    11161 ccatgtacga agaccagcag cctgataact ggcgcgtaca ggcgatactg tatgcatgag
    11221 tgtcatctac agcaaatgaa caatgccgga tgcggcgcga gcgccttgtc cggcctacaa
    11281 ttttcacata tttcatttag ttaatcgaaa ccagcgtcgc atcagtcgta tacgcttaca
    11341 cacacactcc ccccgtcgcc cgcagcaata aatcattctg gatctgctct gacaaccgta
    11401 cgccgccgcg cgtatctaac atcacctgac accacgcctg cgccattggc ggagaagcat
    11461 atttcaacat ttgcgcaccg cagcccagca ggaatagctg atgagtaatc tctcgcccca
    11521 gttcttcagc tggcttacgc agctgctgct gtaaacgacg aaccgcgcga tcaaaatagc
    11581 gatcctgccc tttcacttcc acaaatgctt ccgacaataa gtcgtatacg cccgcttgct
    11641 tattgagaac gcgcaacaca tccaggcaca taatattgcc gg
//
LOCUS       DAZPCS010000116        11496 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZPCS010000116 DAZPCS010000000
VERSION     DAZPCS010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11496
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.116"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(109..2865)
                     /gene="barA"
                     /locus_tag="ACHUQ7_002972"
     CDS             complement(109..2865)
                     /gene="barA"
                     /locus_tag="ACHUQ7_002972"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFR1136282.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLAGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            2922..4223
                     /gene="rlmD"
                     /locus_tag="ACHUQ7_002973"
     CDS             2922..4223
                     /gene="rlmD"
                     /locus_tag="ACHUQ7_002973"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFR1136283.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            4271..6505
                     /gene="relA"
                     /locus_tag="ACHUQ7_002974"
     CDS             4271..6505
                     /gene="relA"
                     /locus_tag="ACHUQ7_002974"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFR1136284.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            6601..7392
                     /gene="mazG"
                     /locus_tag="ACHUQ7_002975"
     CDS             6601..7392
                     /gene="mazG"
                     /locus_tag="ACHUQ7_002975"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFR1136285.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     VNEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            7620..9257
                     /gene="pyrG"
                     /locus_tag="ACHUQ7_002976"
     CDS             7620..9257
                     /gene="pyrG"
                     /locus_tag="ACHUQ7_002976"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFR1136286.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            9345..10643
                     /gene="eno"
                     /locus_tag="ACHUQ7_002977"
     CDS             9345..10643
                     /gene="eno"
                     /locus_tag="ACHUQ7_002977"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFR1136287.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(10703..11496)
                     /locus_tag="ACHUQ7_002978"
                     /pseudo
     CDS             complement(10703..11496)
                     /locus_tag="ACHUQ7_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444999.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="YgcG family protein"
BASE COUNT         2929 a         2768 c         3009 g         2790 t
ORIGIN      
        1 gtaggccgga taaggcgtta acgccgcatc cggcataaac acaggcactt tgtcaccaat
       61 ctgaaaccag cgtcataaaa agccgattgc tactcgacaa gacgtccgtt acccgagaat
      121 tttgctggct tcgcgcgcga cattatccat ctcgtccaac agttccagca gctccggctc
      181 caaatcttct tctttagtac cgctacgtag ctgttgttcg atcagctggc agagattctt
      241 catacgtggt acgccgctat agccgcaact gccatgcagt ttatgaatca aatcaaccag
      301 gccttccggg ttttctccag ccagctgttc ctcaactttg ttgcgaactt caggcaggaa
      361 atcgagtaac atttgcagca tatcgcgcgc taaatcggtt tttcctgctg cctggcgtag
      421 tgccagttgc cagtcgaggg tcgcattcgg gttcaccaca atttcgttga cttcgggcgt
      481 cacgacgcga gacgaaatac cgctgccagg cttgtagcgc aacaacaaat tatgcaatcg
      541 ctcttcttca atcggtttcg ccagataatc gctcatccct gcgccaagca gcttctcttt
      601 ttgcccggcc attgcatgcg ccgttaccgc gataaccggc gtttgtcgct gatgcgggag
      661 ttggtggatg agttcgcagg cccgaatgcc atccatgtcc ggcatttgaa tatccattaa
      721 gatcaaatcg aacggcatct gtttcgcccg ttcaactgcc tgatgcccgc tatcgcaaag
      781 ttccacatgt tgcaccatat cttccagcaa tgcgccgata agtttcaggt tagcggggtt
      841 gtcatcaacc gccatgactg tcattgccag cttactttca tcggttacag gcaaaagcgt
      901 gttttgtttg tgatgacaaa attccgtcag ggcaggcaac aggcgcgtag gtgttaatgg
      961 tttcagcaga cacgcgccga taccatcttg cttgagtttt tcagcattaa cttgtgcatg
     1021 gcaaggaagt gccagcatca ggaaatcggt catcgatacc gctttcgcta atcgctcatg
     1081 ttgcattgtt agcggctcgc ggaaggtcac cgcgatgcct aacaacatca tgtcgtaatg
     1141 cgcgggaggc agcgcggaga acgttgggct ataaaccact tccagcggcg tttcacttaa
     1201 aatatccagc gtgcattgcg ctgctgcgga gtttggttcg acataggcca ggcgcttacc
     1261 tgcgaggcac tggatggatg gcccttcgat aataatgttc gggttcagat cgagattaat
     1321 gtggaaccag aaagttgaac cgcgattcgg ctggctatgg aacgaaatat cgccgcccat
     1381 ttcattaacc agtttttgtg taatcaccag ccccagcccg gtgccaccat gacggcggga
     1441 aatactggca tcagcctgtc ggaaggcctg gaataagcgc gattgatcgc gttcaggaat
     1501 accaatgccg gtatcccgaa tctgcacttc aatctgcact ttggtattac tcagcgcacg
     1561 tttttctacc agaatatcaa tgttgccatt ctcggtgaat ttaattgcat tccccaccag
     1621 gttagtgatg atttgctgta atcgcagtgg gtcgccgatc acgttatcag gcacgtcgct
     1681 tttaatattg agcgtcagtt ccaacccttt atcgtgagaa gaatgtgcca gcagagtaac
     1741 gacttcatcc agcgtgctgc gtagtgggaa tggaatactt tccagaatca gcttacctgc
     1801 ttccagtttc gagaagtcga gaacatcatt aataattgcc agcaaattat ttgccgaacg
     1861 ttcaatcgta ttcaggtgat cgcgctgcgt tggtgttaat tctgttttca gcgtcaggcg
     1921 ggtaaagcca ataacaccat tcagtggtgt acgcagctcg tgtgacatat ttgccagaaa
     1981 ctcggattta atacgcgccg cttcctgggc gcgctttttc gccagatcca actcaacgtt
     2041 ctgaatttcc atctgctcca gcgtttcacg cagatcggac gtcgcctggt cgatattgtg
     2101 ctgcatctct tcgtgataag cagccagcga cattgccatc gagttgatac cgtttttcag
     2161 catatccagc tcgccgagca taaatccttc cacccggctg tcgagttgcc cgcgacggat
     2221 gcggtcgacg gtattcacca tgttgcgaat cggaccggtt acatcgcgca ttaagcgcca
     2281 gccaaaaata agcgcaatac cgatacaaaa cagcatcatc acgcaggaaa taaagatctc
     2341 tttatattgc tgcaagcgaa ccgatttaag atccagctca agcgcaatat atcccaacat
     2401 attttgacta tttttggcat cgctactggg cgattcgtcg ggggagtaac tctcagaaat
     2461 aatcggcgtg cggaggatca taatatcgcc gtcacgagtg acagtgagct ggcgaggaaa
     2521 cggcacgttg ctgccgagct gcattgatga gggatcaaga tgaaaattgg aggtgacaaa
     2581 gagtcggtta ttttcatcat aaaccgaaat cgcgcgaaca atatcggaat ggcgacgatg
     2641 cagtacgctt attaactgac cgatagattc gcgattttgc aggctcatgc catattcagt
     2701 agaaactgca agcggctcaa taatgctagc accggcatct tccagttgac gctgcaagtc
     2761 gttatagcga tgcacgacga aaaagatact cagcaataaa ccaataagga cggtcggtgc
     2821 caggatcaga atcatcatgc gtgcgcgcag gctgtagttg gtcatggagt tccgttatgg
     2881 gacaattaag gtcacactgt taaattgaga aagtctcgcc aatggcgcaa ttctactctg
     2941 caaaacgacg cacgacgacg cgtcagatca taaccgtttc agtcaacgac ctcgactctt
     3001 ttggtcaggg cgtggcgcga cataacggca aaacgttatt tatccccgga ttattgccgc
     3061 aggaaaacgc ggaagttgct gttactgaag ataaaaaaca gtatgcccgc gctaaagtcg
     3121 tacgccggtt aagcgatagc ccggaacgcg aaacgccacg ctgtcctcat tttggcgtat
     3181 gcggcggctg tcagcaacaa cacgccagcg tggatttaca gcagcgaagc aaaagtgcgg
     3241 cactcgcccg attaatgaaa cacgatgtct ctgaagtgat cgccgatgtt ccctggggct
     3301 atcgccgccg cgcgcgttta agtctgaact acttaccgaa aacacagcaa cttcagatgg
     3361 ggtttcgcaa agcgggctcc agtgacattg tcgacgtcaa acaatgcccc attttagcgc
     3421 cccaacttga agcattgctg cccaaagtca gggcatgtct gggcagctta caagctatgc
     3481 gccatcttgg tcatgttgaa ctggtacagg caaccagcgg cacgctgatg attttgcgcc
     3541 ataccgcacc gctaagttcg gcagatcgcg aaaaactgga acgcttttcg cattctgaag
     3601 gcctggatct gtatctcgcc cccgatagtg agatactcga aaccgtctct ggtgagatgc
     3661 cctggtatga ctcaaacggg ttgcgcttaa cttttagccc gcgcgatttt attcaggtca
     3721 atgcgggtgt gaaccaaaaa atggtagcgc gtgcgttgga atggctggat gtgcaacctg
     3781 aagatcgcgt actggatctg ttctgcggta tgggcaactt tacactgcca ttggcgacac
     3841 aagctgccag tgtggtcggt gtagaaggtg ttcctgcgct ggtggaaaaa ggccagcaga
     3901 atgcgcgtct taatggctta cagaatgtga cgttttatca cgaaaatctt gaagaagatg
     3961 tcacaaagca gccgtgggcg aaaaacggct tcgataaagt gttgctggac ccggcgcgag
     4021 caggtgccgc aggtgttatg cagcaaatta taaaactgga acctattcgt atagtttatg
     4081 tatcctgtaa ccctgcaacg ctggctcggg atagcgaagc gttattaaaa gcaggatata
     4141 ccattgcgcg actggcgatg ctggatatgt tcccacacac gggacatctg gaatcgatgg
     4201 tacttttctc gcgcgttaaa tagttacgat ttgccgattt cggcaggtct ggtccctaaa
     4261 ggagaggacg atggttgcgg taagaagtgc acatatcaat aaggctggtg aatttgatcc
     4321 ggaaaaatgg atcgcaagtc tgggtattac cagccagaag tcgtgtgagt gcttagccga
     4381 aacctgggcg tattgtctgc aacagacgca ggggcatccg gatgccagtc tgttattgtg
     4441 gcgtggtgtt gagatggtgg agatcctctc gacattaagt atggacattg acacgctgcg
     4501 ggcggcgctg ctgttccctc tggctgatgc caacgtagtc agcgaagatg tgctgcgtga
     4561 gagcgtcggt aagtcggttg ttaaccttat tcacggcgtg cgtgatatgg cggcgatccg
     4621 ccagctgaaa gcgacgcaca ctgattctgt ttcctccgaa caggtcgata acgttcgccg
     4681 gatgttattg gcgatggtcg atgattttcg ctgcgtagtc atcaaactgg cggagcgtat
     4741 tgctcatctg cgcgaagtaa aagatgcgcc ggaagatgaa cgtgtactgg cggcaaaaga
     4801 gtgcaccaat atctacgcgc cgttggcgaa ccgtcttggg attgggcaac tgaaatggga
     4861 gctggaagat tactgcttcc gttatctcca cccgaccgaa tacaaacgca tcgcaaaact
     4921 gctgcatgaa cgccgtctcg accgcgaaca ctatatcgaa gagtttgtcg gccatctgcg
     4981 cgctgagatg aaagctgaag gcgttaaagc tgaagtgtat ggtcgtccga aacacatcta
     5041 cagcatctgg cgcaaaatgc agaaaaagaa cctcgccttc gatgagctgt ttgatgtgcg
     5101 tgcggtacgt attgtcgccg agcgtttaca ggattgttat gccgcactgg ggatagtgca
     5161 cactcactat cgccacctgc cggatgagtt tgacgattac gtcgctaacc cgaaaccaaa
     5221 cggttatcag tctattcata ccgtggttct ggggccgggt ggaaaaaccg ttgagatcca
     5281 aatccgcacc aaacagatgc atgaagatgc ggagttgggt gttgctgcgc actggaaata
     5341 taaagagggc gcggcagctg gcggcgcacg ttcgggacat gaagaccgga ttgcctggct
     5401 gcgtaaactg attgcgtggc aggaagagat ggctgattcc ggcgaaatgc tcgacgaagt
     5461 acgtagtcag gtgtttgacg accgggtgta tgtctttacg ccgaaaggtg atgtcgttga
     5521 tttgcctgcg ggatcaacgc cgctggactt cgcttaccac atccacagtg atgtcggaca
     5581 ccgctgcatc ggggcaaaaa ttggcgggcg cattgtgccg ttcacctacc agctgcaaat
     5641 gggcgaccag attgaaatta tcacccagaa acagccgaac cccagccgtg actggttaaa
     5701 cccaaacctc ggttacgtca caaccagccg tgggcgttcg aaaattcacg cctggttccg
     5761 taaacaggac cgtgacaaaa acattctggc tgggcggcaa atccttgacg acgagctgga
     5821 acatctgggg atcagcctga aagaagcaga aaaacatctg ctgccgcgtt acaacttcaa
     5881 tgatgtcgac gagttgctgg cggcgattgg tggcggggat atccgtctca atcagatggt
     5941 gaacttcctg caatcgcaat ttaataagcc gagtgccgaa gagcaggacg ccgccgcgct
     6001 gaaacagctt cagcaaaaaa gctacacacc gcaaaaccgc agtaaagata acggtcgtgt
     6061 ggtggttgaa ggtgttggta acctgatgca ccacatcgcg cgctgctgcc agccgattcc
     6121 tggagatgag attgtcggct tcattaccca gggacgcggt atttcagtac accgcgccga
     6181 ttgcgaacaa ctggcggaac tgcgctccca tgcgccagaa cgcattgttg acgcggtatg
     6241 gggtgagagc tactccgccg gatattcgct ggtggtccgc gtggtggcta atgatcgtag
     6301 tgggttgtta cgtgatatca cgaccattct cgccaacgag aaggtgaacg tgcttggcgt
     6361 tgccagccgt agcgacacca aacagcaact ggcgaccatc gacatgacca ttgagattta
     6421 caacctgcaa gtgctggggc gcgtgctggg taaactcaac caggtgccgg atgttatcga
     6481 cgcgcgtcgg ttgcacggga gttagtctta catagctgga tatggcgtca gccgtatcca
     6541 gcgatatctt ttactcgaag gtattacacc tgacagaccc gcaattttca ggacaattca
     6601 atgaatcaaa tcgaccgttt gctcactatt atgcagcgcc tgcgcgatcc ggaaaacggc
     6661 tgcccgtggg ataaagagca gacatttgcc accattgcgc cttacaccct tgaagaaacc
     6721 tacgaagtgc tggacgccat cgcccgtgaa gattttgacg atcttcgcgg tgaactgggc
     6781 gatctgctat tccaggtggt gttttacgcg caaatggctc aggaagaagg gcgctttgac
     6841 tttaatgata tttgcgctgc tattagcgat aaattagagc gtcgccatcc gcatgttttt
     6901 gctgatagtt ctgccgaaaa cagtagtgaa gtgcttgccc gttgggagca aatcaaaacc
     6961 gaagagcgcg cgcagaaagc gcagcattcg gcgctggacg atattcctcg tagtttaccg
     7021 gctttaatgc gggcgcaaaa aatccagaaa cgttgcgcca acgttggctt cgactggacg
     7081 acgcttggcc cggtggttga taaagtctac gaagagatcg acgaggtgat gtacgaggcg
     7141 cggcaggctg ttgtcgacca ggctaaactg gaggaggaaa tgggggacct gctgtttgcc
     7201 acggttaatc tggctcgcca tttagggacg aaagcagaaa tcgcattgca aaaagtgaac
     7261 gaaaaattcg agcgtcgttt tcgcgaagtg gagcgtattg ttgccgcgcg tggactggaa
     7321 atgacaggtg ttgacctcga aacaatggaa gaagtctggc aacaggtaaa acggcaggaa
     7381 attgatctct aagggaatta cgcggtcaag cgccatttgt gtcatttttt aaatgacaag
     7441 cgcttgattt gcgtcaaaaa catttacccc aaaggggcta ttttctcact cctgatttca
     7501 atagtgcgct ggcgaagagg agggataatg aaagtttgtg gcacaggtca tgttcgggta
     7561 tactgctttc ccgtcttggt tattccatcg tcttttcaac ctaacttctc aggttcagca
     7621 tgacaacgaa ctatattttt gtgaccggcg gggtcgtatc ctctctgggt aaaggcattg
     7681 ccgcagcctc cctcgcagcc attcttgaag cccgtggcct caatgtgacc atcatgaaac
     7741 tggatcctta catcaacgtc gatccaggta ctatgagccc aatccaacac ggggaagtgt
     7801 tcgttactga agacggcgct gaaaccgact tggacctggg gcactacgag cgtttcattc
     7861 gtaccaaaat gagccgccgc aacaacttca ccacgggtcg tatctactct gacgttctgc
     7921 gtaaagaacg ccgcggtgac tacctcggcg caaccgtgca ggttattccg cacatcacta
     7981 acgcaatcaa agagcgcgtg ctggaaggtg gcgaaggtca tgacgtagta ctggtagaaa
     8041 tcggcggtac agtaggtgat atcgaatcct tgccgttcct cgaagctatt cgccagatgg
     8101 ctgttgaaat tggccgtgag cacactctgt ttatgcacct gacgctggtg ccgtacatgg
     8161 cagcgtctgg tgaagtcaaa accaaaccga ctcagcactc tgtaaaagag ctgctctcca
     8221 tcggtatcca gcctgacatc ctgatttgtc gttcagatcg cgctgtaccg gcgaacgaac
     8281 gtgcgaagat tgcattgttc tgtaatgttc cggaaaaagc ggttatttct ctgaaagacg
     8341 tcgattccat ctataaaatt ccgggcctgt tgaaatctca ggggctggac gattatattt
     8401 gtaaacgatt cagcttaaac tgcccggaag caaatctgtc cgaatgggaa caggttatct
     8461 tcgaagaagc gaatccggta agtgaagtca ccatcggtat ggtcggcaag tacattgaac
     8521 tgccggatgc ttataaatca gtgatcgaag cactgaaaca cggtgggctg aagaatcgtg
     8581 tcagcgtcaa catcaaactg atcgattcac aagatgttga aacgcgcggc gttgaaatcc
     8641 ttaaaggtct ggacgcaatc ctcgtacctg gcggtttcgg ctatcgtggc gtagaaggca
     8701 tgattacgac cgcgcgtttt gcgcgtgaga acaatattcc ttatctgggc atttgcctgg
     8761 gtatgcaggt ggcgttaatt gattacgctc gccatgttgc caacatggag aacgccaact
     8821 ctacagaatt tgtgccagac tgtaagtacc cggttgtggc gctgattacc gagtggcgcg
     8881 atgaaaacgg caacgttgaa gttcgtagcg agaagagcga tctcggcggt accatgcgtc
     8941 tcggcgcaca gcagtgccag ttggttgacg atagcctggt tcgccagcta tacaatgcgc
     9001 cgacaattgt tgagcgtcat cgtcaccgtt acgaagtcaa caacatgctg ttgaaacaga
     9061 ttgaagatgc aggtctgcgc gttgcgggcc gttccgggga tgatcagttg gtcgagatca
     9121 tcgaagttcc gaatcacccg tggttcgtgg cttgccagtt ccatccggag tttacttcta
     9181 ctccacgtga tggtcacccg ctgtttgcag gctttgtgaa agccgccagc gagttccaga
     9241 aacgtcaggc gaagtaagta aaaaagttag aacggcaacg cgtaccttgg gtacgcgttg
     9301 tttgtctgga gtttcagttt aactagtgac ttgaggaaaa cctaatgtcc aaaatcgtaa
     9361 aaatcatcgg tcgtgaaatc atcgactccc gtggtaaccc gactgttgaa gccgaagtac
     9421 atctggaggg tggtttcgtc ggtatggcag ctgctccgtc aggtgcttct actggttccc
     9481 gtgaagctct ggaactgcgc gatggcgaca aatcccgttt cctgggtaaa ggcgtaacca
     9541 aagctgttgc tgcggtaaac ggcccgatcg ctcaggcgct gattggcaaa gatgctaaag
     9601 atcaggctgg cattgacaag atcatgatcg acctggacgg caccgaaaac aaatccaaat
     9661 tcggcgcgaa cgcaatcctg gctgtatctc tggctaacgc caaagctgct gcagctgcta
     9721 aaggtatgcc gctgtacgag cacatcgctg aactgaacgg cactccgggc aaatactcca
     9781 tgccggttcc gatgatgaac atcatcaacg gtggtgagca cgctgacaac aacgttgata
     9841 tccaggaatt catgattcag ccggttggcg cgaaaactgt gaaagaagcc atccgcatgg
     9901 gttctgaagt tttccatcac ctggcaaaag ttctgaaagc gaaaggcatg aacactgctg
     9961 ttggtgacga aggtggctat gcgccgaacc tgggttccaa cgctgaagct ctggctgtta
    10021 tcgctgaagc tgttaaagct gctggttatg aactgggcaa agacatcact ctggcgatgg
    10081 actgcgcagc ttctgaattc tacaaagatg gtaaatacgt tctggctggc gaaggcaaca
    10141 aagcgttcac ctctgaagaa ttcactcact tcctggaaga actgaccaaa cagtacccga
    10201 tcgtttctat cgaagacggt ctggacgaat ctgactggga cggtttcgca taccagacca
    10261 aagttctggg cgacaaaatc cagctggttg gtgacgacct gttcgtaacc aacaccaaga
    10321 tcctgaaaga aggtatcgaa aaaggtatcg ctaactccat cctgatcaaa ttcaaccaga
    10381 tcggttctct gaccgaaact ctggctgcaa tcaagatggc gaaagatgct ggctacactg
    10441 cagttatctc tcaccgttct ggtgaaactg aagatgctac catcgctgat ctggctgttg
    10501 gtactgctgc aggccagatc aaaactggtt ctatgagccg ttctgaccgt gttgctaaat
    10561 acaaccagct gattcgtatc gaagaagctc tgggcgaaaa agcaccgtac aacggtcgta
    10621 aagagatcaa aggccaggca taagtctgac tttatctgat ttgataatgc cagccttcgg
    10681 gctggcattt tttatggtgg agttaccagc ggccggatgc gccgccactt gagcctccag
    10741 cggaaccgct gttgtctgaa tgataacttc taccagatgc tcttttctga agtacacata
    10801 aacaggtaaa gaccataaat atggtgaaag taaaaaagaa ggaaaataca gcaatggaaa
    10861 aagttttatc ggagaacagg aataaatcta aaagatggat tgctgaaata cagactccac
    10921 ttttacatgc gcgacaaaaa ttactgcctt gatgaaaaaa ccagaaaggg aagaacatca
    10981 ctgcaaatac ccaaaaaata gcaaagtaat aatgatcagt ggaagacgtt gattcacttt
    11041 ctgaaggatt agccggatat tggtgttgtg aagtgagttg gttattcaaa gcgattatag
    11101 ctaactctaa tcccttagct aatttttgtt gtttaaatgc gggtatcatg ttgctacgga
    11161 tgatatctcc agccagagca tcggttacct tttcttccag cccataacct acctggatgc
    11221 ggacagtgcg atccgactag gcaacaacga tcagtatccc atcattacgt ttggcatctc
    11281 ctaaacgcca attgtcaaaa actcttgtag catattgttc aattgtctca tctttggttg
    11341 tggaaacaat taatacagca atttgcactt gggtattatt gaaaatagat tctaacttgt
    11401 gcgtcagttc tttcttttct tgtgagttta agctactcgt aagacccgta acttgttgct
    11461 gtaattgtgg gacaatagtt ggttgggctg caacaa
//
LOCUS       DAZPCS010000117        11407 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZPCS010000117 DAZPCS010000000
VERSION     DAZPCS010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11407
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.117"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            376..815
                     /gene="ycgY"
                     /locus_tag="ACHUQ7_002979"
                     /pseudo
     CDS             376..815
                     /gene="ycgY"
                     /locus_tag="ACHUQ7_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcgY"
     gene            complement(894..2591)
                     /gene="treA"
                     /locus_tag="ACHUQ7_002980"
     CDS             complement(894..2591)
                     /gene="treA"
                     /locus_tag="ACHUQ7_002980"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFR1136288.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNVKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     NYFTMLGLAESGHWDKIADMVANFAHEINTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDSTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATHPTVKSATTQPSTKEAQPTP"
     gene            complement(2911..4329)
                     /gene="dhaM"
                     /locus_tag="ACHUQ7_002981"
     CDS             complement(2911..4329)
                     /gene="dhaM"
                     /locus_tag="ACHUQ7_002981"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFR1136289.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRHLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLGDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            complement(4340..4972)
                     /gene="dhaL"
                     /locus_tag="ACHUQ7_002982"
     CDS             complement(4340..4972)
                     /gene="dhaL"
                     /locus_tag="ACHUQ7_002982"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFR1136290.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            complement(4983..6053)
                     /gene="dhaK"
                     /locus_tag="ACHUQ7_002983"
     CDS             complement(4983..6053)
                     /gene="dhaK"
                     /locus_tag="ACHUQ7_002983"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFR1136291.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            6281..8200
                     /gene="dhaR"
                     /locus_tag="ACHUQ7_002984"
     CDS             6281..8200
                     /gene="dhaR"
                     /locus_tag="ACHUQ7_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HFR1136292.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKLAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            complement(8300..11167)
                     /locus_tag="ACHUQ7_002985"
     CDS             complement(8300..11167)
                     /locus_tag="ACHUQ7_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR1136293.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSSSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IDSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNDSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
BASE COUNT         2745 a         2811 c         2846 g         3005 t
ORIGIN      
        1 cattttatta atgcatttct cattcgggtg aaatttccgc tacctggacg cataaaaccg
       61 gaactctcgc caaaagtcgg gcactccgaa ggaattgacg cgttgcgctg ctctgtaacg
      121 gcatttaccg caagccattt atttcgccag cgacaatccg cagagaacca aacgcatcag
      181 gaaacgactg aacacaataa atactgtatt gatcaacata ttatactggc acctccagca
      241 cgcagtgagg atacactgta tcatcagcga atcttatcac tgcggagctg gttgaacgac
      301 tttctgagcg aaataagtcg gttcccggta acaacaacag aatccttttt taattattgt
      361 ttcgttgttt ttggtgtgat tcagaaaata aatattttag acagtagcca gtcaggcgtt
      421 aaaaatgggg cggatagtga ggcggttttg gctgcgctta ttgaacattt agaattgatc
      481 aatccttctg gcagattaag ccaaaatacc cgttcggcaa tgcttcagtt acgcgaagag
      541 gaatggtctg aattcttttt ttggttgcta aattctctgg agtgtctgga ttatgtgatt
      601 attaatttaa ctccagaaag caaaaaaacg ttgatgagtg aacaccgtaa caatattcgg
      661 tagcaattga tgctttatat agccaacgtc ggcgtaaatc gccgggtgat gaaagtgaaa
      721 cacttacccg tagaaatgac gctatcttcg gcaaccacgt atggcaaaca tttgcgcaat
      781 acttcccgcc aggacttgaa aaaccgtctg tgtagaaaaa accatagata tggtgttatc
      841 tgccgacaac gccatccgga gaagccgaat gacgttgtcg gagtaagcgc tggttaaggt
      901 gtgggttgtg cctctttggt tgagggttgc gtcgttgctg acttaacggt cggatgcgtc
      961 gccggaacat tgtcacacgg ttgctctttc gggcagatca aatccagcat tttcagcgtc
     1021 acgccattgg tccagccaaa gccatcctgt aatggatatt cgccaccgcc gccccccgtt
     1081 ccggtggtgc tgacatcata tttttccacc agctttttct cccggtcata ggtgtgctga
     1141 acattggtca ggaagtgcca gctaatgtcc atcgccacct ctttttgccc gtagttttgt
     1201 aatccttctg tcgcgaccca ctgtaacggt gcccagccat ttggcgcatc ccattgttgc
     1261 ccacttttca ccgacgtggt gttcaggccg ccgggttgca gcagatgtgt tttcgtcgcc
     1321 gtcgccattt tgttggcgcg atctttcgct gccgcattga cgtacagcgg gaacagggcg
     1381 gccgcggtta actgattgcg cactttatga cttttcaggt cgtaatcggc ataccagcct
     1441 tgttgatcgt tccacaggta tttttcgatc cctttttgac gggcattcgc cagcgtttcg
     1501 tactggtttg ccatcgcgtt atctcctgca gctttgctgg cgcgggcgag gattttttcc
     1561 attttaaaca tcaggctgtt cagatcgacc ggtacgatgc tggtggtgcg taaggtattt
     1621 aactgctgcg ggttgtccat ccagcgcgag ctgaaatccc agccagacgc agcggcagag
     1681 cgcaggtcgc ggtaaatttc agtggcaggt cgattcggat tgcttttggc ggtggcaata
     1741 tcttccaccc atgactctgg tcgtggcgta tcgcgatcgt cccagtagcg gttgagaagg
     1801 gtactatcct gaagttttac aacgcgtttt tcctgttgtc cggcttgcag gttttcaaca
     1861 ccgtccatcc agtaagcata ttctttttgc atttgcggca ggtattgctt caacgcggca
     1921 tcgccttcat gctgcgccag taactctacc atcagggcaa agaagggcgg ttgcgagcgg
     1981 cttaaatagt aactgcggtt gccgttggga atatgaccgt aagtgtttat ttcatgagca
     2041 aaattggcca ccatatccgc gattttatcc cagtgaccac tttcggcaag tcctaacatg
     2101 gtgaagtaat tgtcccagta atatacctcg cgaaaacgtc cgcccggcac gacataaggt
     2161 tccggcagcg gtaacagaga atcccatttt tcggtgtttt cggtagaacg cgttaatacc
     2221 ggccaaagtc cgtcaatatg ttcgcgcagt gactgcccct ctggcggaac atatttctcg
     2281 ccttctttcg gcagggtgaa attgacgtta acgaaatggc gcagatcaaa tccgctctgg
     2341 ttttgctgca tccgataatc agcaaggatc atcagcggat cgctgttagg cacggcatcg
     2401 gcaaaggttt tttggtccgg aaaaagtttg acgttttgca catcattaaa cagcggccct
     2461 aataaaatat caggcggctg tggtgttacc ggtgtttctt ctgcctgcac cgatagcgca
     2521 gcgaaacaca aaaagataca ggctggaatt aacgccattt tttgcgggcg agaaggtgcg
     2581 ggggatttca tcaatcattc tcctttggcg aaaccgaata aaacggtatt caagtatcag
     2641 aaaaccttag ttcaggatcg cactagctgc atgatcgaaa tctcattctg cgaacggtca
     2701 ggcattttag aaaagggaat taaagggata aataacggcg aaatagagtg atcaaataac
     2761 gtctgtttat tagccagcca gcgacatttg gttcgcgatt agcgaagaaa aatcatcata
     2821 aattggtaaa aaatataaca ggtagttaaa aaacatcagt gctgggtaaa ttgccggatg
     2881 acatcagaac gatgccatcc gaacggtggc ttaaccctga cggttgaaac gttgcgtttt
     2941 aacgtccagc gttagcgttt cttctggttg tatcgcatac agtttctcac cctgctggca
     3001 aatccagcca atccccagtt cacgggcgat tagggcgctg tgggataccg gacttccggc
     3061 gctaaggcag atacctttta caaccgccgg atccagttgc agtactgtgg aaggataaat
     3121 gttctccgcc agtagaatag ttggcgagtt aaactgcggg agttcttctt tcgtttgggt
     3181 caggtggacc agggtgcgat gcagaagatc gtccacatca atatagcgag cttgtagata
     3241 ttcatcatcc agttgctggt attgctggct aagttcttta agaacttgct gccaggcata
     3301 ttctgccgtg caatgttcat gctgaaggag ttcgcttgcc gccgccagca gttccggatc
     3361 acctaacagt gtatggtgac cagaaaagat tgcggcaata tcgtcaagcc cgctggcttc
     3421 tgctttcgct gttaacgtca tcagatctaa taacgtgaag tcaatagcct ggcgtaatcg
     3481 atcttgttct tcttccgcgg tcagggttga ttttgcctgt accgtacata aaactggttg
     3541 ataataaaag gctttacccg aaacaggcag aacgggacgc agagtaggtg gagcgacttc
     3601 ctccgtttca ccaaagttat cttcagccag gtgacggaaa gcgatcagtg cctcttcagc
     3661 ttctggccct ttcgcaatca ggcgcagcgt atcgttatag cgaacttgta gtaacgcaat
     3721 ctggttaata ctctctggtg tgacgcattt gccgtttttt tccagcaaca tatcggcatt
     3781 aaatgtcgat aaggtataaa ccagccggga ggccggacgt acatgcaggc cgttacggtt
     3841 ttttatgacc accgccagag aacgggcttc ttcatcgtac ggaggacatg tgtcagagat
     3901 ttcagtgtcg gaggacggta aacccagttg ttcacgtttg gcttccagcg catgcatagc
     3961 gtcaaagata actttgtcga tatccgcccc cgaggccgcg ctgaccgttg ctgccagtgt
     4021 accttcgacc aacggcgcag cacacaaacg tacttttgcg gcgatctcgg gagccagcaa
     4081 ttccagcgca gtttcagcac tcaataatgc gctacccata tccatcatga ccagcacatg
     4141 gtcggcatca gcaacagatt cgatggcctc catcactttg acggcatcgg taccaatggg
     4201 attttgtgga tcgtcaattc ccgcggcaat ggcgatttta caactatcac tcattaacat
     4261 ctgacgggct aattcaccga caccttctcc cagtcggctg ctatgtgaaa ctatgaccag
     4321 gtttaccatc accaattcct tactcttttg cggctaacgc caacatttgc atcataaaca
     4381 tcaccgaggt cgcgccggga tcctggtgac caatactgcg ttcaccgaga taactggcgc
     4441 ggcctttgcg ggcttgcatc gtaatcgtac tttgtgcagc ggattcggcg atgctactgg
     4501 cagcttcgag cgccaccgga acagagagat tttgctcgct ggactgacgt aacgattcca
     4561 ccaccggcac ccacacatca cacatggttt tatcgccagg ttcggctttc ccgcgactga
     4621 ttacgccgtc cgcgccatcg cggaacatct gataaagctc ttccagtgtc aggctttgcc
     4681 gtgcctgggt cgcctgtgcg gcgcggataa agaaggtacc gaacagcgga ccactggcac
     4741 caccgacgct ggaaagcagc gtcataccgg tatttttgag aatgaaaccg atatctttat
     4801 ctgcgatagc agggagtttt tccaccactt tgctaaagcc tcggttcata tttagcccgt
     4861 ggtcagcgtc gccaatctcg cgatccagcc cggtaagata ctcgctctcg gtgttgaaaa
     4921 tatcgccaca acgagtgagc cagttaacaa tttgagttct gctcagtgac attgctttct
     4981 ccttatttac cccagttaag ggccggggtg tggaccgggg cgtcccagag agccagcgtt
     5041 tcgtcatcaa ctttcagtaa ggtgattgag aaaccggtca tatccagtga ggtgcagtac
     5101 gcgccaatta aattacgttc gatagtcaat cccgcttgct ggcaacgtgt ggtcaggcgg
     5161 ttatagacgc cgtacagctc agaaagcgga gttgcgccaa gattgttaac cagtgcaatc
     5221 acccgatcgc cagactggag cggttgtttg gtttgtggtt cttcctgcca gctgccttgt
     5281 tgataatccc agaaacgcaa agtgcgatgg tatgagccat gttccagcag ggtgtcgaac
     5341 atttcatcga cggtttgatc aagggaagag aaagggcggc ggtcaatacc tggctcacca
     5401 tgaatgccta cgccaaactc catctcatta tccgcaaggg taaaagaagg tttgcccgcg
     5461 gcaggaacgg tacaggcacc gagagcgata cctattgagt ggccttgatt attcaactta
     5521 cgccccagtt ccgcacaggc gtccagtgag tcgccacgct ccgccgctgc gcctacgagt
     5581 ttttcaatta atacggtgtt ggcaacgccg cgtcgcccgg cggtataaag actgtctttt
     5641 accgcaacgt cgtcatcaat gaccacagtg gtcactttta cgccgctatc gtgcagtaac
     5701 tcggtcgctg tttcaaagtt aagaatatcg ccggtgtaat ttttgataat caacagtaca
     5761 ccttcgccgc catcaatttg catggagcat tcaaagattt tatcgggcgt cggtgaggtg
     5821 aaaatttcgc ccggacaggc ccccgaaagc atcccctgac cgatataacc acagtgcatc
     5881 ggctcgtgtc cgctgccgcc acccgacagc agggcgactt ttcctgcaac aggggcatca
     5941 gctcgggtga catacaccgg atcctgatgc agtgtcagcg atggatgcgc tttcgccagt
     6001 cctgccagtt gttcgtccag tacgtcttgc acatcattga tcaatttttt cattattttg
     6061 ctccagcaat tacggtaggg catggatgat gttcaacgac acggcgacca ctgactgccg
     6121 atgaatccat tgtgcatcag gcaagggaaa agaaaattag cccaaattat gtttcatagt
     6181 gaaacatatg ctttaatgaa tgttccatat tgaaactttt acgtgtatta atacttaaaa
     6241 ttgcgagccg gaacaccttt tgtcataagg gatgcgggat atgagtggcg cttttaacaa
     6301 cgatggtcgg ggcatatctc ccttaattgc aacctcctgg gagcgatgca ataagctgat
     6361 gaaacgggag acatggaacg taccacatca ggcccagggc gtgacatttg cttctattta
     6421 tcggcgtaag aaagcgatgc tgacgctcgg gcaggctgcg ctggaagatg cctgggaata
     6481 tatggcaccg cgagagtgtg cgctgtttat cctcgatgaa accgcctgca ttctcagccg
     6541 taatggcgat ccgcaaacct tgcagcagct aagtgcactg ggattcaatg acggcacgta
     6601 ttgcgccgag ggaattattg gtacttgtgc gctatcgtta gcggctatct ctggtcaggc
     6661 cgtgaaaacg atggccgatc aacatttcaa acaggcactc tggaactggg ccttttgtgc
     6721 aacgccgttg tttgacagca agggccgatt gacgggaaca atagcgctgg cgtgtccggt
     6781 tgagcaaact accgcagctg atttgccgtt gacgttggca atcgcccgcg aggtcggtaa
     6841 tttactgctg acggacagtt tgctcgctga aactaaccgt catttaaatc aacttaatgc
     6901 cctgttagaa agtatggatg atggcgtgat tagctgggac gagcagggta atttgcaatt
     6961 tattaatgcc caggcggcgc gggtcttgcg ccttgacgcg acggcaagtc agggacgggc
     7021 aatcactgaa ctcttaacgt tacccgccgt attgcaacaa gcaataaaac tggcacatcc
     7081 gctcaaacac gtagaagcaa cctttgaaag ccagcaccag tttattgatg cggtgataac
     7141 ccttaaaccg ataatagaaa cgcagggaac cagctttatt ttgttgctcc atcctgtgga
     7201 acagatgcgg cagttaatga ccagtcaatt aggaaaagtc agccatacct tcgctcatat
     7261 gccacaggac gatccgcaaa cccgccgctt gattcatttt ggtcgccagg cggcgcgcag
     7321 tagctttcct gtcctgcttt gtggagaaga gggcgtgggc aaggcactgc taagtcaggc
     7381 aattcataat gaaagcgagc gtgctgcagg tccttatatc gccgtcaatt gtgagttata
     7441 tggtgatgct gcgctggcgg aagaatttat tggtggcgat cgcacggaca atgaaaatgg
     7501 ccgtctgagt cggctggaac tggcacacgg cggcacgctg tttcttgaaa agattgaata
     7561 tctggcggtg gagttacagt ctgctttgct gcaggttatc aagcaggggg ttatcacgcg
     7621 actggatgcg cggcgtttaa taccaattga tgtcaaagtg attgcaacaa cgaccgcgga
     7681 cctcgcaatg ctggtggaac aaaatcgttt tagtcgccag ctgtattacg cgctgcatgc
     7741 atttgaaatt accatcccgc ctctgcgtat gcggcgtggc agcattccgg cgctggtgaa
     7801 taacaaatta cgcagtcttg aaaaacgctt ctctacgcgg ctgaaaattg atgacgatgc
     7861 cctcgctcgc ctagtttctt gtgcatggcc aggcaacgat tttgaacttt acagcgtcat
     7921 cgagaatctt gctctgagta gtgataacgg gcgcattcgc gtcagtgatt tacctgaaca
     7981 tctgtttacc gagcaggcga cagatgatgt cagcgccacc cgcctttcca ccagtctgtc
     8041 atttgcggaa gttgaaaaag aggcaattat taacgcagcc caggtcacag gcggtcgcat
     8101 tcaggaaatg tcggctttac ttgggatcgg ccgcactacg ctgtggcgga aaatgaagca
     8161 acatggcatt gatgcagggc agtttaagcg ccgggtatga aagacagaaa cgatttctga
     8221 tacatcagag tgatctgtat ttcattccgg cgcacgctaa caattttcag catcgtttaa
     8281 gggcttgtct atcccgcact taaaagctga agcgatatcc tccgttgact tgcttctgat
     8341 caaagaggtt accctgcgtg taatccgctt cgagatagaa tgtgtgctgt ttgttatact
     8401 gtgcactgac tcccacgccg ttattccagc cattaccttt gaaactgtac ttctcccggg
     8461 agtcgttcaa cagatattcg gtatcccctg aaaactcgcg gatagcgcca gtcttcacat
     8521 agacattcag ctggctgttg cctgcggtga tgtcataccc cagtatcatg ctggcacgcc
     8581 ccagcagcga ttcgtagtga ttcagatgta tattgaggcc attactcgcc ttcatagcca
     8641 tctcattctg gtggctgtat gtaagctggg tttgcggctc tatatagaac ccataaccag
     8701 taggggacag gttgaacctc tgcccggctt ccagggagat gctcattcca ttcgcagtgc
     8761 cgttggcgtt aacgccgttg ttctgactgt ccagtacgtg gaaactattt ttctggcgcg
     8821 atgcttttat aacgagatcg ctgtaaaaac cgttttgtgc catgtaactg gcgtacattc
     8881 ccatgtagtc tgaacgtgcg gtaccgtcgc ctccgctata gtccggcgat gcatgtgttg
     8941 agtcaatata cagaccgaca tacaacggca ttacatcaga gagacgttta tccccaccaa
     9001 actggatacc gctgtaaccc atgtcaaagc cgctcagttt gccactggca aaggagtcca
     9061 ggcttccccc ataactgcgc aaccagatat taccgtcttt actctgattt cgcaagtcac
     9121 ccatccgttg catcagcgta cggttttcaa catagttcaa taagtaaccg acattgagat
     9181 aattgccgcc agcatctgcg gtcgtggtgg gtttaggcgt gggagcgggt tcaggcgtag
     9241 gatcggggtt gactatggga ggctgagcgg gagctggtgt gggttcagga ttaggagtcg
     9301 gttccggaac tgtcccggaa gcgtaaagct cccagttagt gccattttta cgcacatcgt
     9361 acagatatcc ccccaactca acctgagaag acgcgctgaa cgaggccgcg ccgtcagtgg
     9421 tttttaccac tgtcagaact tcatttcccg ttgtggcctc gctgccctgg ttgcggatag
     9481 ccaatacgtg attaccagca ctactcccgc tgatattcaa taaatcccct ctattattaa
     9541 cgccattacc ctcgccaaca acatcagcac gcataataaa ggtactgtta ccgctcaggt
     9601 tctctacgtt taatgtggta aatgtgccgg cagttgaccc gtggctggca aaatcgacag
     9661 ttgaatggct cagcgccagc gtgtcgagat tagaattact tgttacgttc cagacgctgt
     9721 tattcattgc aacgtccagt ttcccgccat tgacattatc gctgagggag gaacctgtcc
     9781 aaaccgaccc agggtgcata tccagattga ttagcccacc tttggaaaga acgctaccgt
     9841 tgataagcat acgacccgag gcgttgatgc ggctggcggc ataaccatcg tcatgttgcg
     9901 ttgcgatggc catctggtcg ggtgtactca tatcaatgac cagatcgctc gtgaggtcga
     9961 tctggctgtt ggtcatggca taaatccctc tggctcctgc cgcgccggtg atagccaaac
    10021 tgtctccggt tattctaccg ccgctgagcg cccacaaacc cagcgccaga ctgccattac
    10081 gatcaaccgt aatatcggta ttttgcatgt tgataacagc cgttgccgtc tgggccgagg
    10141 caccataaga accgccggaa aagatgctgt ttcgttgcgc tgccgtgcca gaaaaattga
    10201 ttgtcgcgtc tgaactactg gtgacgaggc caccgccctg cgcggaagaa atatggctat
    10261 ctgcaccgat agtggttgta ccaccacgaa cttcgacgcc attggccgcg gctccagtta
    10321 catcaacagt gagtgcattc gcgctaacct ggccaaagct ccagaggccg tgagcattat
    10381 cgccattggt tttaatggta ctgtttgttc cgagatcgac aacagagttt ttctgtacgt
    10441 ttatccccat ggcgctgtag ccctgaacat cgattgtcag gtctgtcgcc gtaaaacgcg
    10501 cagcaccatt ggcgttattg ccgttgagac caccgatata aacacctgta cttccatcgg
    10561 tcttgatttt acttccgctt ccaagatcga cactggtgcc atagtcattg atggttaggc
    10621 ctataccgtt cgagttttca atggtgaatt gagtggctgt tagtgttgag ctatgcccaa
    10681 taatgatgcc gtcatcgtta ctcttaatgg tgctgcctgt gcctaagtca gcatgggtat
    10741 agtcaccaat taagttaatg ccgatggcag aggtttgacc aacaacatct actgtgagtc
    10801 ggttagctgt cagggagctg tttttgccct gaataatgat tccttttgca tatccagagg
    10861 cgtcgttgac attgacggtg acatcattac ccaggttgat attgccaggc gtgtttcctg
    10921 ctggggtcat tacaccatag aggtttcctg atggatcggc ggtatctcct gtaattctgt
    10981 caccatcatt aatagtaata ttggtgccat attgacttcc tgtatcagca gctatcagca
    11041 ggggggaaaa gataattgca ttcaaaccaa tagcgccaaa ttttatcagt tgaattgagg
    11101 gcgaacggat ttttaattca gcgagacgat cggctttggt attcccattg tgttgtttga
    11161 tgcccatacc actcctatat agtacccagg ctatgggtct atggattttg tcattttgtt
    11221 taagtcaatg aataatatcc tcttatcatc atgatatctg tatggtgcaa tcatgatgag
    11281 cttctctgag aataattctc tattaccggg caaaatcagg aacacaggaa ttaatatata
    11341 tttaaccccc cccttacaaa ggtgagttta tatcgagtta ttccctaagt tgtcgttact
    11401 tattcca
//
LOCUS       DAZPCS010000118        11346 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZPCS010000118 DAZPCS010000000
VERSION     DAZPCS010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11346
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.118"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            241..540
                     /locus_tag="ACHUQ7_002986"
     CDS             241..540
                     /locus_tag="ACHUQ7_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFR1136294.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(660..839)
                     /locus_tag="ACHUQ7_002987"
     CDS             complement(660..839)
                     /locus_tag="ACHUQ7_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFR1136295.1"
                     /translation="MFAGLPSLIHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGELIVARFEDEDE"
     gene            913..2274
                     /gene="pabB"
                     /locus_tag="ACHUQ7_002988"
     CDS             913..2274
                     /gene="pabB"
                     /locus_tag="ACHUQ7_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFR1136296.1"
                     /translation="MKTLSPTVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTHEQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2278..2856
                     /locus_tag="ACHUQ7_002989"
     CDS             2278..2856
                     /locus_tag="ACHUQ7_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFR1136297.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINREPLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVTP"
     gene            3040..4404
                     /gene="sdaA"
                     /locus_tag="ACHUQ7_002990"
     CDS             3040..4404
                     /gene="sdaA"
                     /locus_tag="ACHUQ7_002990"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFR1136298.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4535..6133
                     /locus_tag="ACHUQ7_002991"
     CDS             4535..6133
                     /locus_tag="ACHUQ7_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFR1136299.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSKVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVIEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTH
                     YVMAETIRQHHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMSLR"
     gene            complement(6137..7687)
                     /gene="yoaE"
                     /locus_tag="ACHUQ7_002992"
     CDS             complement(6137..7687)
                     /gene="yoaE"
                     /locus_tag="ACHUQ7_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFR1136300.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIFEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(7675..7833)
                     /gene="yoaL"
                     /locus_tag="ACHUQ7_002993"
     CDS             complement(7675..7833)
                     /gene="yoaL"
                     /locus_tag="ACHUQ7_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFR1136301.1"
                     /translation="MDRHRRHFSIRPFNACLSGTLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            8150..9121
                     /gene="manX"
                     /locus_tag="ACHUQ7_002994"
     CDS             8150..9121
                     /gene="manX"
                     /locus_tag="ACHUQ7_002994"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFR1136302.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            9184..9984
                     /gene="manY"
                     /locus_tag="ACHUQ7_002995"
     CDS             9184..9984
                     /gene="manY"
                     /locus_tag="ACHUQ7_002995"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFR1136303.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            9997..10848
                     /gene="manZ"
                     /locus_tag="ACHUQ7_002996"
     CDS             9997..10848
                     /gene="manZ"
                     /locus_tag="ACHUQ7_002996"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFR1136304.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10903..>11346
                     /locus_tag="ACHUQ7_002997"
     CDS             10903..>11346
                     /locus_tag="ACHUQ7_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFR1136305.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRIPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLL"
BASE COUNT         2785 a         2866 c         2852 g         2843 t
ORIGIN      
        1 tcgccctttg attagcaact ctggttttct ttcttagctg aacaatctgg ttatagcgtg
       61 cgggctgacc gttaattttc tccatcttac tggataacac accatgagct gctaccttta
      121 aaacacctga atgtcaacag gttaactcgt gccatatcgt tataatccct ttgatattag
      181 atgcaaatta aggtcatata tagccttatc gataacatgg ttaattttaa ggataagaat
      241 atgcctgctg taatagataa agccctggat ttcattggtg ccatggatgt atcagcgcca
      301 acaccaagtt cgatgaatga aagcacggcg aagggaatct ttaaatattt aaaagaactg
      361 ggagtacccg cgagtgccgc tgatattacg gcgcgagctg accaggaagg ctggaaccct
      421 gggttcacgg aaaaaatggt tggatgggca aaaaaaatgg agacaggtga acgctctgtg
      481 attaaaaatc ctgaatactt ttcaacatat atgcaggaag aactaaaagc actggtctga
      541 gttaaattta tatcagcata aatgggtcag gacgctttta atcacatata aaaagcgtcc
      601 tttttctcac aatcaaatga tcatcggctt ctataacgaa atcgatacgg attagtgtgt
      661 tactcgtctt catcctcaaa acgcgccacg attagctcgc cggagtggtt ggcacgcagc
      721 tcttctgcca ccagcgcgat tgcctggccg ctactcatcc cttgtgccat cagttcctgg
      781 atccgctcga cagctttttg ctgctgttca tggatgagtg aaggtaaacc tgcaaacatt
      841 gttaactcct gctaaattgt tggcgctaat tatttcatgc tacccggcac atagccagta
      901 gagtcaggac tgatgaagac gttatctccc actgtgatta ctttaccctg gcgtcaggac
      961 gccgctgaat tttatttctc ccgcttaagc cacctgccgt gggcgatgct tttacactcc
     1021 ggctatgccg atcatccgta tagccgcttt gatattgtgg tcgccgatcc gatttgcact
     1081 ttaaccactt taggtaaaga aaccgttgtt agtgaaagcg aaaaacgcac aacgaccact
     1141 gatgacccgc tacaggtgct ccagcaggtg ctggatcgcg cagacattcg cccaacgcat
     1201 aacgaagatt tgccatttca gggcggcgca ctggggttgt ttggctacga tctgggccgc
     1261 cgttttgagt cactgccaga aattgcggaa caagatatcg ttctgccgga tatggcagtg
     1321 ggtatctacg attgggcgct cattgtcgac caccagcgtc atacagtttc tttgctgagt
     1381 cataatgatg tcaatgcccg tcgggcctgg ctggaaagcc agcaattctc gccgcaggaa
     1441 gatttcacgc tcacttccga ctggcaatcc aatatgaccc acgagcagta cggcgaaaaa
     1501 tttcgccagg tacaggaata tctgcacagc ggtgattgct atcaggtgaa tctcgcccag
     1561 cgttttcatg cgacctattc tggcgatgaa tggcaggcat tccttcagct taatcaggcc
     1621 aaccgcgcgc catttagcgc ttttttacgt cttgaacagg gtgcaatttt aagcctttcg
     1681 ccagagcggt ttattctttg tgataatagt gaaatccaga ctcgcccgat taaaggcacg
     1741 ctaccacgcc tgcccgatcc tcaggaagat agcaaacaag cagaaaaact ggcgaactca
     1801 gcgaaagatc gtgccgaaaa tctgatgatt gtcgatttaa tgcgtaatga tatcggtcgt
     1861 gttgccgtag ccggttcggt aaaagtacca gagctcttcg tggtggaacc cttccctgcc
     1921 gtgcatcatc tggtcagcac tataacggcg cgactaccag aacagttaca cgccagcgat
     1981 ctgctgcgcg cagcttttcc tggtggctca ataaccgggg ctccgaaagt acgggctatg
     2041 gaaattatcg acgaactgga accgcagcga cgtaatgcct ggtgcggcag cattggctat
     2101 ttgagctttt gcggcaacat ggataccagc attactatcc gcacgctgac tgccattaac
     2161 ggacaaatat actgctctgc gggcggtgga attgtcgccg atagccagga agaagcggaa
     2221 tatcaggaaa cttttgataa agttaataag atattacgcc aactggagaa gtaagacgtg
     2281 gaataccgta gcctgacgct tgatgatttt ttatctcgct ttcaactttt gcgcccgcaa
     2341 attaaccggg aacccctaaa tcatcgtcag gctgccgtgt taatccccat cgtccgtcga
     2401 ccgcaaccgg ggttgttgct gactcagcgt tcaattcatc tgcgtaaaca cgctggacaa
     2461 gtggcattcc ctggaggtgc agtcgatgac acggacgcat cagttatcgc cgccgcgctg
     2521 cgcgaagctg aagaagaggt cgctataccg ccttccgccg ttgaagttat cggcgtgctg
     2581 ccgcccgtcg atagcgtaac tggctatcaa gtaaccccag tggtcggcat tatcccgccc
     2641 gatctgccgt atcgcgccag tgaagatgaa gtctcggcgg tgtttgaaat gccgctcgcc
     2701 caggcattac atctgggtcg ttatcaccct ttagatatct accgccgtgg cgattcacat
     2761 cgggtatggc tgtcctggta cgaacagtat tttgtatggg gaatgaccgc aggcataatt
     2821 cgtgagctgg cgctgcaaat tggtgtgacc ccctgactat acttatcttt acatctacaa
     2881 aacactactt gagacaatca tcgcaatatt agttaaatcg cggtttttga ttagtttaat
     2941 tcatgtgaat agttaagcca gtcgccgcgt tccctcttac actatgcgct gttattagtt
     3001 cgttactgga agtccagtca ccttgtcagg agtattatcg tgattagtct attcgacatg
     3061 tttaaggtgg ggattggtcc ctcatcttcc cataccgtag ggcctatgaa ggcgggtaaa
     3121 cagttcgtcg atgatctggt cgaaaaaggc ttactggata gcgttactcg cgttgccgtg
     3181 gacgtttatg gttcactgtc gctgacgggt aaaggccacc acaccgatat cgccattatt
     3241 atgggtcttg caggtaacga acctgccacc gtggatatcg acagtattcc cggttttatt
     3301 cgcgacgtaa aagagcgcga acgtctgctg ctggcacagg gacggcatga agtggatttc
     3361 ccgcgcgaca acgggatgcg ttttcataac ggcaacctgc cgctgcatga aaacggtatg
     3421 caaatccacg cctataacgg cgatgaagtc gtctacagca aaacttatta ttccatcggc
     3481 ggcggtttta tcgtcgatga agaacacttt ggtcaggatg ctgccaacga agtgagcgtg
     3541 ccgtatccgt tcaaatctgc caccgaactg ctcgcgtact gtaatgaaac cggctattcg
     3601 ctgtctggtc tcgctatgca gaacgaactg gcgctgcaca gcaagaaaga gatcgacgag
     3661 tatttcgcgc atgtctggca aaccatgcag gcatgtatcg atcgcgggat gaacaccgaa
     3721 ggtgtactgc caggcccgct gcgcgtgcca cgtcgtgcgt ctgccctgcg ccggatgtta
     3781 gtttccagcg ataaactgtc taacgatccg atgaatgtca ttgactgggt aaacatgttt
     3841 gcgctggcag ttaacgaaga aaacgccgcc ggtggtcgtg tggtaactgc gccaaccaac
     3901 ggtgcctgcg gtatcgttcc ggcagtgctg gcttactatg accactttat tgaatcggtc
     3961 agcccagaca tctatacccg ttactttatg gcagcgggcg cgattggtgc attgtataaa
     4021 atgaacgcct ctatttccgg tgcggaagtt ggttgccagg gcgaagtggg tgttgcctgt
     4081 tcaatggctg ctgcgggtct tgcagaactg ctgggcggta gcccggaaca ggtttgcgtg
     4141 gcggcggaaa ttggcatgga acacaacctt ggtttaacct gcgacccggt tgcagggcag
     4201 gttcaggtgc cgtgcattga gcgtaatgcc attgcctctg tgaaggcgat taacgccgca
     4261 cggatggctc tgcgccgcac cagtgcaccg cgcgtctcgc tggataaggt catcgaaacg
     4321 atgtacgaaa ccggtaagga catgaacgcc aaataccgcg aaacctcacg cggtggtctg
     4381 gcaatcaaag tccagtgtga ctaatacttc ttactcgccc atctgcaacg gatgggcgaa
     4441 tttatacccg ctttctcgtc tgctgtaata ttccccacta cacttccact gttgcgtcag
     4501 gcgtttgtcg ccatacgctt acagggtggc ccgcatgcaa aaagcacaac ggatcattaa
     4561 aacctatcgc cgtaatcgaa tgattgtttg tacgatttgc gcactcgtta cgctcgcttc
     4621 gaccctgagc gtgcgattta tttcacagcg taacttaaat caacaacggg tagtacaatt
     4681 cgccaatcac gctgtagagg aattagataa agtactgctt cccctacagg caggtagcaa
     4741 agtcttgctt ccgctgattg gtctgccctg ctctgtcgcc catttgccat tacgtaaaca
     4801 ggcggcaaaa ctccaaactg tgcgatccat tggcctggtg caagacggca cactttattg
     4861 ctccagcatt tttggttatc gcaatgtgcc cgtcgtggac attctggctg aacttcctgc
     4921 accgcaacca cttttacgcc tgacgatcga ccgtgccctg attaaaggca gtccggtttt
     4981 gattcaatgg acgcctgcag cgggcagtag caatgctggg gtcatagaga tgattaacat
     5041 cgacttactg acggcaatgc tccttgagcc acaactgccg caaatcagta gcgccagcct
     5101 gacggtggac aaacggcatt tgctctatgg taatgggctg gtagattccc ttccgcaacc
     5161 tgaagacaat gaaaactatc aggtttcttc gcaaagcttt ccttttacca ttaacgtaaa
     5221 tggtccgggg gctacggcgc tggcatggca ctatcttcca acacaattac cgctggcggt
     5281 gctgctaagt ttactggtgg gctatatcgc ctggctggcg accgcttacc ggatgagctt
     5341 ttcccgcgaa attaatctgg gcctggcgca acatgagttc gaattgttct gtcagccttt
     5401 gcttaatgcg cgcagccagc aatgtattgg tgtagagatt ttgctgcgct ggaataatcc
     5461 gcgtcagggc tggatttcac cggatgtgtt tattcctatc gcggaagaac atcatttaat
     5521 tgtgccactg acccactatg tgatggcaga aaccattcgt cagcaccatg ttttcccgat
     5581 gagtagtcag tttcatgttg gcattaacgt cgcacccagc cattttcgcc gtggtgtgct
     5641 gataaaagat ctcaatcagt actggtttag cgctcacccg attcagcaac tgatcctcga
     5701 aatcaccgaa cgcgatgcct tactggatgt tgattatcgg attgcccgcg agctgcatcg
     5761 taaaaacgtc aaactggcga ttgatgactt cggcaccggc aacagctcgt tttcctggct
     5821 tgaaacatta cgtcctgacg tgctgaaaat tgataagtca tttaccgcag ctataggttc
     5881 tgacgcggtt aactcgacgg tgaccgatat catcatcgcg ctggggcaaa gactgaatat
     5941 tgaactggtg gcggagggcg tggaaacgca agaacaggcg aagtatttgc gccgtcatgg
     6001 cgtgcatatt ttgcaagggt atttgtacgc acagccgatg ccgctacgtg attttcccaa
     6061 atggctggcg ggcagccaac cgccgcccgc ccggcataat ggacatatca cgcccattat
     6121 gtcgttacgt taacgcttac tcatcttcat cgtgcgccgg ttgctcttta acaatgcgaa
     6181 ccagatcaac acgataatca ttggcttcaa agatggtgat atgcagtggc cctacatcaa
     6241 tcacatcgcc cacacgggga atgtgaccat ttgccgagat cacgaggccc gcgaccgtcg
     6301 cgatatcatc gtcatcggca aggtgctcaa catcaagcgc ctgctgcaag gcatgcaaat
     6361 ctgtaccgcc ttttaccagc cagccgttac catcggtaat gatttccggc gtttcgtcag
     6421 cgtccgggaa ttcacccgca atggcttcca gcacatccag cggcgtgacc aagccttgta
     6481 ccacaccaaa ctcgttggtc acgataacaa agctcccgcg agcgcgacgc agcacgccca
     6541 gcaggttgat cggatcgagg gtttccggga cgataatcgc cggagacgcc gaagcaatcg
     6601 ccgccacatc aacgccctct tccagcgcca ccagcagttc tttagcacgt acgataccga
     6661 tgatttcatc cagttcaccg cgacataccg ggaacagact gtgcggtgaa gagagcagtt
     6721 gctcgcggat ttcatcgacc ccgagattag cgtcaaccca gcttatttca ccgcgcggcg
     6781 tcatgatccc gcgcagagaa cgcgacgcca gcgtcagtac gccgttaatc atgtaccgtt
     6841 cttcttcggc aaatgcacct tccgggatcg gcatcgggtt atcggcatcg tgctgaacat
     6901 tggcctgacg tttcccgccc atcaaacgca ggatggcatc ggcagtacgc gctcgcagcg
     6961 gcaaagtcga ctggtgacga ataaagttgc gacgcgcaat ctggttaaac acttcgataa
     7021 tgatcgagaa gccaatcgcg gcatacaggt aacctttcgg aatgtggaaa ccgaaacctt
     7081 ctgccaccag actcagacca atcattaaca ggaagctcag acagagcacc accaccgtgg
     7141 ggtgctggtt aacgaatcgc gtcagcggtt tcgatgccag caacataacc gccatcgcaa
     7201 tcactaccgc cgccatcatc accggcagat ggttaaccat ccctactgca gtaattaccg
     7261 catccaacga gaagacggcg tcaaggatga caatctgtgt gacgaccacc cagaaactgg
     7321 cgtagccttt accgtggccg gaatcatgat cgcggttttc cagccgttca tgcagttcgg
     7381 ttgttgcttt gaacagcaag aatatccccc cgaacaacat aatcagatcg cgtccggaga
     7441 aggagaaatc catgacggta aatagcggtt tggtcagcgt gaccatccat gaaatcagcg
     7501 acagcagccc cagacgcata atcagcgcca gtgataaccc cagcaaacgc gctttatcgc
     7561 gttgttttgg cggcagtttg tcagcaagaa tggcgatgaa gaccaggtta tcgataccca
     7621 gcacaatttc gagaacaaca agcgtgagta gccccgccca aattgagggg tccattaaga
     7681 attccatgac aagctcctgc ttaaggaata gctattcgac gccagaaata atgcaggcgt
     7741 aacgacaaaa tgcaaacgaa aggtgcggca tagagtgcca gaaaggcagg cgttaaaagg
     7801 cctgatgctg aaatgacgtc ggtgacgatc catactgcgg gctactgccc tatactccat
     7861 ggttgttaaa cgggagttaa acatatcaga gacgcctctg atttggcaaa gatttacctt
     7921 cctttgcaaa cgaatgtgac aaggatattt tacctttcga aatttctgat aatcgaaagt
     7981 taaattacgg atcttcatca cataaaataa ttttttcgat atctaaaata aatcgcgaaa
     8041 cgcaggggtt tttggttgta gcccttatct gaatcgattc gattgtggac gacgattcaa
     8101 aaatacatct ggcacgttga ggtgttaacg ataataaagg aggtagcaag tgaccattgc
     8161 tattgttata ggcacacatg gttgggctgc agagcagttg cttaaaacgg cagaaatgct
     8221 gttaggcgag caggaaaacg tcggctggat cgatttcgtt ccaggtgaaa atgccgaaac
     8281 gctgattgaa aaatacaacg ctcagttggc aaaactcgac accactaaag gcgtgctgtt
     8341 tctcgttgat acatggggag gcagcccgtt caatgctgcc agccgcattg tcgtcgacaa
     8401 agagcattat gaagtcattg caggcgttaa cattccaatg ctcgtggaaa cgttaatggc
     8461 ccgtgatgat gacccgagct ttgatgaact ggttgcgctg gcagtagaaa caggccgtga
     8521 aggcgtgaaa gcactgaaag ccaaaccggt tgaaaaagcc gcgccagcac ccgctgccgc
     8581 agcaccaaaa gcggctccaa ctccggcaaa accaatgggg ccaaacgact acatggttat
     8641 tggccttgcg cgtatcgacg accgtctgat tcacggtcag gtcgccaccc gctggaccaa
     8701 agaaaccaat gtctcccgta ttattgttgt tagtgatgaa gtggctgcgg ataccgttcg
     8761 taagacactg ctcacccagg ttgcacctcc gggcgtaaca gcacacgtag ttgatgttgc
     8821 caaaatgatt cgcgtctaca acaacccgaa atatgctggc gaacgcgtaa tgctgttatt
     8881 taccaaccca acagatgtag agcgtctcgt tgaaggcggc gtgaaaatca cctctgttaa
     8941 cgtcggtggt atggcattcc gtcagggtaa aacccaggtg aataacgcgg tttcggttga
     9001 tgaaaaagat atcgaggcgt tcaaaaaact gaatgcacgc ggtattgagc tggaagtccg
     9061 taaggtttcc accgatccga aactgaaaat gatggatctg atcagcaaaa tcgataagta
     9121 acgtattgtg ttgattatca ttcagttttc acacttaagt cttacgtaaa caggagaagt
     9181 acaatggaga ttaccactct tcaaattgtg ctggtattta tcgtagcctg tatcgcaggt
     9241 atgggatcaa tcctcgatga atttcagttt caccgtccgc taatcgcgtg taccctggtg
     9301 ggtatcgttc ttggggatat gaaaaccggt attattatcg gtggtacgct ggaaatgatc
     9361 gcgctgggct ggatgaacat cggtgctgca gttgcgcctg acgccgctct ggcttctatc
     9421 atttctacca ttctggttat cgcaggtcat cagagcattg gtgcaggtat cgcactggca
     9481 atccctctgg ccgctgcggg ccaggtactg accatcatcg ttcgtactat taccgttgct
     9541 ttccagcacg ctgcggataa ggctgctgat aacggcaacc tgacagcgat ttcctggatc
     9601 cacgtttctt ctctgttcct gcaagcaatg cgtgtggcta ttccggccgt catcgttgcg
     9661 ctgtctgttg gtaccagcga agtacagaac atgctgaatg cgattccgga agtggtgacc
     9721 aatggtctga atatcgccgg gggcatgatc gtggtggttg gttatgcgat ggttatcaac
     9781 atgatgcgtg ctggctacct gatgccgttc ttctacctcg gcttcgtaac cgcagcattc
     9841 accaacttta acctggttgc tctgggtgtg attggtactg ttatggcagt gctctacatc
     9901 caacttagcc cgaaatacaa ccgcgtagcc ggtgcgcctg ctcaggcagc tggtaacaac
     9961 gatctcgata acgaactgga ctaacaggtg agcgaaatgg ttgatacaac tcaaactacc
    10021 accgagaaaa aactcactca aagtgatatt cgtggcgtat tcctgcgttc taacctcttc
    10081 cagggttcat ggaacttcga acgtatgcag gcactgggtt tctgcttctc tatggtaccg
    10141 gcaattcgtc gcctctaccc tgagaacaac gaagctcgta aacaagctat tcgccgtcac
    10201 ctggagttct ttaacaccca gccgttcgtg gctgcaccga ttctcggcgt aaccctggcg
    10261 ctggaagaac agcgtgctaa tggcgcagag atcgatgacg gtgctatcaa cggtatcaaa
    10321 gtcggtttga tggggccgct ggctggtgta ggcgacccga tcttctgggg aaccgtacgt
    10381 ccggtatttg cagcactggg tgccggtatc gcgatgagcg gcagcctgtt aggtccgctg
    10441 ctgttcttca tcctgtttaa cctggtgcgt ctggcaaccc gttactacgg cgtagcgtat
    10501 ggttactcca aaggtatcga tatcgttaaa gatatgggtg gtggcttcct gcaaaaactg
    10561 acggaagggg cgtctatcct cggcctgttt gtcatggggg cattggttaa caagtggaca
    10621 catgtcaaca tcccgctggt ggtttcgacc atcaccgggc aggatggaca gacccgtgtc
    10681 accaccgtgc aaactattct tgaccagtta atgccaggcc ttgtaccact gctgctgaca
    10741 tttgcttgta tgtggctgct gcgcaaaaaa gttaacccgc tgtggatcat cgttggcttc
    10801 ttcgtcatcg gtatcgctgg ttacgcttgc ggcctgctgg gactgtaaga ctgtcgtaca
    10861 ctaccggggc cttttggccc cgttttttta tctggaggat taatgacaat cacggacctg
    10921 gtactgattc ttttcatcgc cgcactcctg gccttcgcga tctacgatca gttcatcatg
    10981 ccccgccgta acggccccac cctgctggca attcctttgc tccggcgtgg tcgcatcgat
    11041 agcgttatct tcgtcggatt gattgtcatt cttatctata acaacgtcac gaatcatggc
    11101 gcgctaataa caacatggtt attaagtgca ctggctctga tgggttttta tatattctgg
    11161 atccgcattc cgaagatcat ctttaaacaa aaaggttttt tcttcgccaa tgtctggatt
    11221 gaatatagcc gaatcaaagc gatgaacttg tcggaagatg gcgtgttggt gatgcaatta
    11281 gaacagcgtc ggctgttaat ccgcgttcga aatatcgacg atctggaaaa aatttataag
    11341 cttctc
//
LOCUS       DAZPCS010000119        11330 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZPCS010000119 DAZPCS010000000
VERSION     DAZPCS010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11330
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.119"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..611)
                     /gene="hdhA"
                     /locus_tag="ACHUQ7_002998"
     CDS             complement(<1..611)
                     /gene="hdhA"
                     /locus_tag="ACHUQ7_002998"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFR1136306.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLDGQAFAWRCDITSEQELSALADFAVSNLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNSIAPGAILTDALKSVITP"
     gene            complement(723..1751)
                     /gene="malI"
                     /locus_tag="ACHUQ7_002999"
     CDS             complement(723..1751)
                     /gene="malI"
                     /locus_tag="ACHUQ7_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFR1136307.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLNGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1926..3518
                     /gene="malX"
                     /locus_tag="ACHUQ7_003000"
     CDS             1926..3518
                     /gene="malX"
                     /locus_tag="ACHUQ7_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFR1136308.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            3528..4700
                     /gene="malY"
                     /locus_tag="ACHUQ7_003001"
     CDS             3528..4700
                     /gene="malY"
                     /locus_tag="ACHUQ7_003001"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFR1136309.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCNPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4804..5805
                     /gene="add"
                     /locus_tag="ACHUQ7_003002"
     CDS             4804..5805
                     /gene="add"
                     /locus_tag="ACHUQ7_003002"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFR1136310.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLRYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5841..6881)
                     /locus_tag="ACHUQ7_003003"
     CDS             complement(5841..6881)
                     /locus_tag="ACHUQ7_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFR1136311.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            7124..7249
                     /gene="blr"
                     /locus_tag="ACHUQ7_003004"
     CDS             7124..7249
                     /gene="blr"
                     /locus_tag="ACHUQ7_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFR1136312.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            7522..7737
                     /gene="ydgT"
                     /locus_tag="ACHUQ7_003005"
     CDS             7522..7737
                     /gene="ydgT"
                     /locus_tag="ACHUQ7_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFR1136313.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7823..8263
                     /locus_tag="ACHUQ7_003006"
     CDS             7823..8263
                     /locus_tag="ACHUQ7_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFR1136314.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            8340..8921
                     /gene="rsxA"
                     /locus_tag="ACHUQ7_003007"
     CDS             8340..8921
                     /gene="rsxA"
                     /locus_tag="ACHUQ7_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFR1136315.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLTSICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            8921..9499
                     /gene="rsxB"
                     /locus_tag="ACHUQ7_003008"
     CDS             8921..9499
                     /gene="rsxB"
                     /locus_tag="ACHUQ7_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFR1136316.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRVMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            9492..>11330
                     /gene="rsxC"
                     /locus_tag="ACHUQ7_003009"
     CDS             9492..>11330
                     /gene="rsxC"
                     /locus_tag="ACHUQ7_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFR1136317.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQTNGTPLRQVLLAQHFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKATRLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAEREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIARS
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPE"
BASE COUNT         2644 a         2921 c         2858 g         2907 t
ORIGIN      
        1 tctggtgtaa taacggattt cagggcatcg gttaatattg cccccggcgc aatgctattc
       61 acccgaatat ttttttcacc caggtcaaat gccatatttc tgaccagatg actggccgca
      121 gctttagatg atgcatagga agtcatgttt atatttttat tttctgccgc catagaagtg
      181 atggtcagaa taacgccacc tccatttttt tccatttctg gcgcaacaag ttgtgacaga
      241 tggaaaaaag aaaacacatt cagttcataa gcacggcgaa aatctgccat tggcatatca
      301 aacggtttag gaccaccgcc accggcgttg ttaaccagga tatcaacttt acccagatta
      361 ctgacggcaa agtctgccag tgcagagagt tcctgttcgg aagtaatatc acaacgccag
      421 gcaaatgcct gaccatccag ttgttgaatt tcgtctacaa catggttagc tgcgtcggcg
      481 ttaatatcac tgaccaccac agatgcgcca gctgtcgcga atgtaatggc gatttcttta
      541 ccaatacctg cacccgcacc tgtgatgatg gcgcattttc cgtcgagtct caggttgtca
      601 gaattaaaca cataaacctc ctgtgaactt cagtaaaaat aaaaggcacc gcctgtgtgc
      661 cacatttttt atcattaacc ttgaggaaag gacactacca agtatagcag ataaagagag
      721 gattatttcg ccgcaatgag ccgggcggga ataataagat tgcgtgaatg cgtctcttca
      781 tgggtgattt tttgcatcat gcgatccgca agtgtgatac caagttcccg cgctggcgtg
      841 ctggcccagg taacaggtat atcatcaagt gtggttggtg tcgcatcggt aaatgccgcc
      901 agcgaaacct gttgctcaaa gtaacgatcg actccgcttt ccccgctttg ccgccctgct
      961 ttcagcaaac caaaccatgc ccccatcgca atagtttcgt tatagcaaac cacggcactg
     1021 atggtcgggt tatgacgtaa aagcgccgtg atagcttccg cggcttgctt ctggctggaa
     1081 gtgcactcca acacccaatc gctgtgaaac ggcaggccaa attttagtag agttgcacaa
     1141 tagcccccca cccgttctgc acgggttaat gaggaacttt gccctcccag ccaggcgatc
     1201 cgctgatgcc cattgcgaat gagatgctcc gtcaacaact gtgcagcctg catgttgtcc
     1261 gggcgaaccg tatcaacatc atcaagataa ctggcacggg aagcgaaaat caccgggata
     1321 gctttttctt ctgccatccg ccgcaggtca tcgctgcttc ctgcagcccc ggcaattacc
     1381 acaccatcga caccctgatt cagtaacagt gaaaaccgct gtgccagctg ctcgccgtct
     1441 ttaccgccgt gaagcaaaaa aaccatccgt ccctgtgctt ccagagcttc cgtcaatccg
     1501 gccgtcaatt cggcgtaaaa cggcgcagat aaatcacgga cgatcaaacc aatgacgccg
     1561 ctttgcccgc cgcgcagcgc cgacgcctgg cgattgcgca caaatcccag ctcttcaatg
     1621 gcggcgttaa cgcgttctcc tgtggcggta gagattcgcc ctttgccatt aagcaccagc
     1681 gaaacggtac ttaccgacac gcccgcagcc agcgcaacat catgaatggt tatttttttg
     1741 gcggtagcca tgcagatgac ctactccctg attatgtgac agataaaacg ttttaccttt
     1801 tatttatctt atacccgcta ttatcgttgc gtaatgtgat ttatgactca ctaaaatttg
     1861 ataaaacgtt ttatcttctc gcgcaattta ctgaatccag attgttctct acgaggagtc
     1921 gttttatgac ggcgaaaaca gcaccgaaag tcacgctgtg ggagttcttc cagcagttag
     1981 gcaaaacctt catgttaccc gtggcattat tgtcgttctg cggcattatg ctcggcattg
     2041 gtagttctct tagcagccat gatgtcataa ccctgatccc ggtcctgggc aaccccgtgt
     2101 tgcaggctat ctttacctgg atgagtaaga ttggctcgtt tgcttttagt ttcctgcctg
     2161 tcatgttctg tatcgccatc ccgctgggcc tggcacgcga aaataaaggc gtagcggcat
     2221 tcgctggctt cgtcggttat gcggtaatga acctcgcggt aaacttctgg ttgaccaata
     2281 aaggcattct gccaaccaca gatgccgccg ttctgaaagc caataacatc cagagcattc
     2341 ttgggatcca gtcgatcgat accgggatcc tcggtgcggt gatcgccggt attatcgtct
     2401 ggatgctgca tgagcgtttc cataatatcc gcctgccgga tgcgctggca ttcttcggcg
     2461 gtacgcgctt cgtaccaatt atctcctcgc tggtgatggg ccttgtcggc ctggtgattc
     2521 cattagtctg gccgattttc gccatgggta ttagcggctt gggccatatg ataaacagcg
     2581 cgggtgattt cggaccgatg ctgtttggta ccggtgaacg tctgctgttg ccgtttggtc
     2641 tgcatcacat tctggtggca ttaattcgct ttaccgacgc aggcggcacg caggaggtct
     2701 gcggtcaaac cgtcagcggc gcactgacca tcttccaggc gcaattgagt tgcccgacca
     2761 ctcacggttt ttctgaaagc gtcacgcgtt tcctttcgca aggtaaaatg cctgcgtttc
     2821 tcggcggtct accaggtgca gcgttagcta tgtatcactg cgcgcgcccg gaaaatcgcc
     2881 ataaaattaa aggtctgctg atttctggcc tgatcgcctg cgtcgttggc ggcactaccg
     2941 aaccgctgga attcctgttc ctgttcgtag cgccagttct gtatgtcatc cacgcgctgt
     3001 taaccggcct cggcttcacc gtcatgtctg tgctcggcgt caccatcggt aataccgacg
     3061 gcaatatcat cgacttcgtg gtgttcggta ttttgcatgg tctgtcaacc aagtggtaca
     3121 tggtgccagt ggtggcggca atctggtttg tcgtttacta cgtcatcttc cgtttcgcta
     3181 tcacccgctt caatctgaaa accccggggc gcgatagcga agttgccagc tcaatcgaaa
     3241 aagccgttgc cggtgcgccg ggtaaatcag gttacaacgt tcctgcaatc ctcgaagcat
     3301 taggcggtgc cgacaatatt gtcagcctcg ataactgcat tacccgtctg cgtttgtctg
     3361 tggaagatat gtcgcttgtt aatgtgcagg cactgaagga caatcgggca attggcgtag
     3421 tacaacttaa tcaacataac ctgcaggttg ttatcgggcc acaagttcag tcagtaaaag
     3481 atgaaatggc cggtctgatg catactgtcc aggcataagg ataagatatg ttcgattttt
     3541 caaaggtcgt ggatcgtcat ggcacatggt gtacacagtg ggattatgtc gctgaccgtt
     3601 tcggcactgc tgacctgtta ccgttcacga tttcagacat ggattttgcc actgccccct
     3661 gcattatcga ggcgctgaat cagcgcctga tgcacggcgt atttggctac agccgctgga
     3721 aaaacgatga gtttctcgcg gctattgccc actggttttc cacccagcat tacaccgcaa
     3781 ttgatacgca gacggtggtg tatggccctt ctgtcatcta tatggtttca gaactgattc
     3841 gtcagtggtc tgaaacaggt gaaggcgtgg tgatccacac gcccgcctat gacgcatttt
     3901 acaaggccat tgaaggtaac cagcgcacag taatgcccgt tgctttagag aagcaggctg
     3961 acggctggtt ttgcgatatg ggcaagctgg aagccgtttt ggcgaaacca gaatgtaaaa
     4021 ttatgctcct gtgtaaccca cagaatccta ccgggaaagt gtggacgtgc gatgagctgg
     4081 agatcatggc tgacctgtgc gagcgtcatg atgtgcgggt tatttccgat gaaatccata
     4141 tggatatggt ctggggcgag cagccgcata ttccctggag taatgtggcg cggggagact
     4201 gggcgttgct gacatcggga tcgaaaagtt tcaatattcc cgcgctgacc ggtgcttacg
     4261 ggattataga aaatagcagc agccgtgatg cctatttatc ggcactgaaa ggacgtgatg
     4321 ggctttcttc cccttcggta ctggcgttaa ctgcccatat cgccgcctat cagcaaggtg
     4381 caccgtggct ggatgcctta cgcgtctatc tgaaagataa cctgacgtat atctcagata
     4441 aaatgaacgc cgcgtttcct gaactcaact ggcagatccc gcaatccact tatctggcct
     4501 ggcttgatct acgtccgttg aatattgacg acaacgcgtt gcaaaaagcg cttatcgagc
     4561 aagaaaaagt cgcgatcatg ccggggtata cctatggtga agaaggtcgt ggttttgtcc
     4621 gtctcaatgc cggctgccca cgttcgaaac tggaaaaagg tgtggctgga ttaataaacg
     4681 ccatccgcgc tgttcgctaa ccccaattgc gcaacgtaaa aaatcgttgc gcaatcgtgg
     4741 atttttaccc tgctttgttt ttataatggt gcgcactttt atatccagaa aaagagtgcg
     4801 accatgattg ataccaccct gccattaact gatatccatc gccaccttga tggcaacatt
     4861 cgtccccaga ccattcttga acttggccgc cagtataata tctcgcttcc tgcacaatcc
     4921 cttgaaacac tgattcccca cgttcaggtc attgccaacg aacccgatct ggtgagcttt
     4981 ctgaccaaac ttgactgggg cgttaaagtt ctcgcctctc ttgatgcctg ccgccgcgtg
     5041 gcatttgaaa acattgaaga tgcagcccgt aacggcctgc gctatgtcga gctgcgtttt
     5101 tcaccaggct acatggcaat ggcacataag ctgcctgtag cgggtgttgt cgaagcggtg
     5161 atcgatggcg tacgtgaagg ttgccgcacc tttggtgtgc aggcgaagct tatcggcatt
     5221 atgagccgaa ccttcggcga agccgcctgt cagcaagagc tggaggcctt tttagcccac
     5281 cgtgaccaga ttaccgcact tgatttagct ggtgatgaac ttggattccc gggaagtctg
     5341 ttcctttctc acttcaaccg cgcgcgtgat gcgggctggc atattaccgt ccatgcaggc
     5401 gaagctgccg ggccggaaag catctggcag gcgattcgtg aactgggtgc ggagcgtatt
     5461 ggacatggcg taaaagccat tgaagatcgg gcgctgatgg attttctcgc cgagcaacaa
     5521 attggtattg aatcctgtct gacctccaat attcagacca gcaccgtagc agagctggct
     5581 gcacatccgc tgaaaacgtt ccttgagcat ggcattcgtg ccagcattaa cactgacgat
     5641 cccggcgtac agggagtgga tatcattcac gaatataccg ttgccgcgcc agctgctggg
     5701 ttatcccgtg agcaaatccg ccaggcacag attaatggtc tggaaatggc tttcctcagc
     5761 gcagaggaaa aacgcgcact gcgagaaaaa gtcgccgcga agtaacaaaa atggatggtg
     5821 caaattgcac catccatttt tcacgcaagg cacaacgtcg cgcgatgttt ggcggattcg
     5881 atgcccagct caatcaactc cattacctgg attgcctggc ttgccggaac cggattttca
     5941 ccatcgccat ttaacgcatc acgaatcgcc gcatagtaag ccggataatt cccaggcacc
     6001 gtcaacagcg tttcttcgac acgttcctca ccttccacgc gggtaagtac gccatcacgc
     6061 atatcgtagc cccagtcttc ctgcggcaga cgctcgccat ttttcagacg ttcttcctgt
     6121 ggatcgaggc catatttcac ataactgcct cgggatccat gcacgatata ccgtgctgac
     6181 tcagcggctg ccagcatggt accgtgtaaa atgactcgcc gctgtggata ggacaagatg
     6241 gcgtggaaat aatcggtcga ctgcgctccg ggccgtaact gtgccaaatc taccgtcatg
     6301 ctgaccggta aaccaaatag cgtaatggcc tgatcaagaa gatgcggtgc taaatcgtac
     6361 cagataccgc tgcctgggcc gccctgttca cgccaacgat cgcgcacctg cggacggaag
     6421 cggtcaaaat gagactcaaa gtaagcaact tcacccagca cgccttccgc gagcaaacct
     6481 tttagcgtca agaaatcgct atcccaacgg cggttatgga atacagacag cacacgcccc
     6541 aggctttttg ccagcgcatc cagctctcgc gcttgtgaca gtgtcacggt aaagggttta
     6601 tcaacgacca catgtttacc cgcctcaagc gccgctttgg ctaacgggaa atgggtatcg
     6661 ttgggtgtag gaatgacaat caggtctatg ttgggatcgt taaacagatg cttcggctca
     6721 gagacaaccg taaccgttgg ccagtcggct tttacttttg tttcatcact gctggagatt
     6781 accgccagtt ccagcccggg cgtgcccgca atcaggggcg catggaaggt tttgctcgca
     6841 taaccatacc caatcaaccc aacacggatg ttgtcgctca tgtcattgcc tctcattgaa
     6901 gtatgatggc tatttgacac tatcctttac ccacgctcaa cagtttaata acctgccagc
     6961 aataagggat gttgtttaac ttaagtcaaa aaaatagcga attttccaac gacaaaagct
     7021 aaatatcgca aaaacctcag taaaaatctt gctggagcta ttattgctaa gtaacattta
     7081 ccccctgaag ttaatggatc aatcaagaga gatgtgggct gtaatgaatc gtcttattga
     7141 attaacaggt tggatcgttc ttgtcgtttc agtcattctt cttggcgtgg cgagtcacat
     7201 tgacaactat cagccacctg aacagagtgc ttcggtacaa cacaagtaag ctctgcactt
     7261 gtggagcgac atgctgcccg tccgggtgca tgttttcact tgtcggatat taaaccagga
     7321 atttattatc ttgttcgatg ttgttggtga ttgtcaggga tagtaaagcg cgttactctt
     7381 ttcagagcag tttttcatcc ctgaatattt gccatcagcg cattcttatt attgtaataa
     7441 cacaagcgtt cacttttggt gaaattttat catcactaat aatacctacc cctttggtat
     7501 taattatttt atggaccttt tatgactgtt caggactact tattaaaatt tcgcaaaatc
     7561 agttcactcg aaagtctgga aaaactctac gaccatctta attacaccct gacggacgat
     7621 caggaactga tcaatatgta tcgtgctgcc gatcaccgtc gcgcagagct ggtttctggc
     7681 gggcgtttgt ttgacctcgg ccaggtaccg aagtccgtct ggcactatgt ccaataaaga
     7741 aagtagcgat cggttataaa agccttataa taacgcccct aaaattagat ttgcctgttt
     7801 gcgtcgctat tctggagaac ctatgaccac cacaacgcca caacgtattg gaggctggtt
     7861 gcttggcccg ttggcctggc ttttagtcgc tctgttgagt acgacgctgg cgttgttgct
     7921 gtataccgct gcgttatctt ctccgcaaac atttcaaacg ctcggcggac aagcactgac
     7981 gacacaaatc ttatggggcg tttcttttat taccgctatc gccatgtggt attacacact
     8041 gtggttgacc atcgctttct ttaaacgtcg gcgttgtgtt cctaaacact atattatctg
     8101 gctgcttatt tccgtattgc tggcagtaaa agcattcgct ttttcacccg tggaagttgg
     8161 catagccgtt cgccagttgc tgtttacttt gttggcaacc gcattgatcg tcccctattt
     8221 caaacgctcg tcgcgggtaa aagcgacgtt tgtgaatccg taataacctt acagttaacc
     8281 tgttgtcgcc tgctctggat taacggataa taggcggctt ttttatttca ggccgaaaaa
     8341 tgactgacta cctgttactg tttgtcggaa ctgtactggt caataacttt gtactggtca
     8401 agtttctcgg tctctgtccg tttatggggg tttccaaaaa actggaaacc gcgatgggca
     8461 tggggctggc aacaacgttt gtgatgacgc tgacgtctat ttgcgcctgg cttatcgata
     8521 cgtggatttt gatcccactt aatctgattt acctgcgcac cctggcattt attctggtga
     8581 ttgctgtggt cgtgcagttc accgagatgg tggtgcgcaa aaccagcccg gtgctttacc
     8641 gtctgctggg gatttttttg ccgcttatca ccaccaactg tgcagtgctc ggcgtggcgt
     8701 tgctgaatat caatctcggg cacaatttct tgcagtcggc gctgtacggt ttttccgccg
     8761 ctgtcggttt ctcgctggtg atggtgctct tcgccgccat ccgcgaacgc cttgctgtgg
     8821 ctgatgtccc ggcacctttt cgcggtaatg ccattgcgtt aattaccgca ggtcttatgt
     8881 ctctggcctt tatgggcttt agtggtttgg tgaagttgta atgaatgcta tctggattgc
     8941 cgttgccgcc gtgagcctgc tgggcctggc gtttggcgcc attctgggtt atgcctcccg
     9001 ccgttttgcg gtggaagacg atccggtcgt tgagaaaatt gacgaaatct taccgcagag
     9061 ccagtgtggt cagtgcggtt atcccggctg tcgcccctac gcggaagcca tcagctgtaa
     9121 cggtgaaaaa atcaaccgtt gcgccccagg tggcgaagct gtgatgctaa aaattgccga
     9181 gttgcttaat gtcgagccgc agccgctgga tggcgaagcg caagagctaa cgcctgcgcg
     9241 gatggtggcg gttattgatg aaaataactg tattggctgc actaaatgta ttcaggcttg
     9301 tccggtagac gccatcgttg gcgctacccg tgtcatgcat acggtaatga gtgatctctg
     9361 tacgggctgc aatttatgtg ttgatccgtg cccgacgcac tgcatctcgt tgcaaccggt
     9421 cgcagaaaca cctgactcct ggaaatggga tctgaacacc attcccgtgc gtatcattcc
     9481 cgtggaacac catgcttaag ttattctctg cattcagaaa aaataaaatc tgggatttca
     9541 acggcggcat ccatccaccg gagatgaaaa cccagaccaa cggtaccccc ctgcgccagg
     9601 tactactggc gcagcatttt gttattccac tgaaacagca tattggcgct gaaggtgagt
     9661 tgtgcgttag cgtcggcgat aaagtattgc gcggccagcc gcttacccgt ggtcgcggca
     9721 aaatgctgcc tgttcacgcg cccacctcgg gtaccgttac ggctattgcg ccccactcta
     9781 cggctcatcc ttcagcttta gctgaattaa gcgtgattat tgatgccgat ggtgaagact
     9841 gctggatccc gcgcgacggc tgggtcgatt atcgctctcg cagtcgcgaa gagttaatcg
     9901 agcgcataca tcagtttggt gttgccgggc tgggcggtgc cggattcccg acaggcgtta
     9961 aattgcaggg tggtggagat aagattgaaa cgttgattat caacgctgct gagtgcgagc
    10021 cgtacattac cgccgatgac cgtttgatgc aggattgcgc ggctcaggtc gtagagggta
    10081 ttcgcattct tgcgcatatt ctgcagccac gcgaaattct tatcggcatt gaagataaca
    10141 aaccgcaggc gatttccatg ctgcgcgcgg tgctggcgga ctctaacgat atttctctgc
    10201 gggtgattcc aaccaaatat ccttctggcg gtgctaaaca attaacctac attctgaccg
    10261 ggaagcaggt tccacatggc gggcgttcat ccgatatcgg cgtattaatg caaaacgtcg
    10321 gcactgctta tgcagtgaaa cgtgccgtta ttgatggcga gccgattacc gagcgtgttg
    10381 taaccctgac tggcgaagca atcgctcgcc cgggcaacgt ctgggcacgg ctggggacgc
    10441 cagtgcgtca tttattgaat gatgccggat tctgcccctc tgccgatcaa atggtgatta
    10501 tgggtggccc gctaatgggc tttaccttgc catggctgga tgtcccggtc gtaaagatta
    10561 ccaactgtct gttggctccc tctgccaatg aacttggcga accacaggaa gaacaaagct
    10621 gcatccggtg tagcgcctgt gctgacgcct gccctgtgga tcttttgccg caacagttgt
    10681 actggttcag caaaggtcag caacacgata aagctaccac gcataacatt gccgactgca
    10741 ttgaatgtgg ggcttgcgcg tgggtttgcc cgagcaatat tcccttggtg caatatttcc
    10801 gtcaggaaaa agctgaaatt gcggctattc gtcaggaaga aaagcgcgcc gcagaagcca
    10861 aagcgcgttt cgaagcgcgc caggctcgtc tggagcgcga aaaagcgact cgccttgaac
    10921 gacataagag cgcagccgtt caacctgcag ccaaagataa agatgcgatt gctgccgctc
    10981 tggcgcgggt gaaagagaaa caggcccagg ctacacagcc tattgtgatt aaagcgggcg
    11041 aacgcccgga taacagtgca attattgcag aacgggaagc ccgtaaagcg caagccagag
    11101 cgaaacaggc agaactggag caaactaacg acgcagcaac cgttgctgaa ccacgtaaaa
    11161 ctgccgttga agcagctatc gcccgctcca aagcgcgcaa gctggaacag caacaggcta
    11221 atgcggaacc agaagaacag gtcgatccgc gcaaagccgc cgtcgaagcc gctattgccc
    11281 gtgccaaagc gcgcaagctg gaacagcaac aggctaatgc ggaaccagaa
//
LOCUS       DAZPCS010000120        11251 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZPCS010000120 DAZPCS010000000
VERSION     DAZPCS010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11251
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.120"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            1..135
                     /locus_tag="ACHUQ7_003010"
     CDS             1..135
                     /locus_tag="ACHUQ7_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFR1136318.1"
                     /translation="MIYYLGYDRFLLIFFTDYSHFMDRITLPSTDTASGKGKWKIITA
                     "
     gene            114..218
                     /gene="speFL"
                     /locus_tag="ACHUQ7_003011"
     CDS             114..218
                     /gene="speFL"
                     /locus_tag="ACHUQ7_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HFR1136319.1"
                     /translation="MENNNRLMPHIRRTTHIMKFTHRNSFDFHFFNAR"
     gene            529..2726
                     /gene="speF"
                     /locus_tag="ACHUQ7_003012"
                     /pseudo
     CDS             529..2726
                     /gene="speF"
                     /locus_tag="ACHUQ7_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
     gene            2723..4042
                     /gene="potE"
                     /locus_tag="ACHUQ7_003013"
     CDS             2723..4042
                     /gene="potE"
                     /locus_tag="ACHUQ7_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFR1136320.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFIANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGGIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4098..5738)
                     /gene="pgm"
                     /locus_tag="ACHUQ7_003014"
     CDS             complement(4098..5738)
                     /gene="pgm"
                     /locus_tag="ACHUQ7_003014"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFR1136321.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDKANALMADGLKGVKRISLDEAMASGYVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5764..6309)
                     /gene="seqA"
                     /locus_tag="ACHUQ7_003015"
     CDS             complement(5764..6309)
                     /gene="seqA"
                     /locus_tag="ACHUQ7_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFR1136322.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            6494..7258
                     /gene="ybfF"
                     /locus_tag="ACHUQ7_003016"
     CDS             6494..7258
                     /gene="ybfF"
                     /locus_tag="ACHUQ7_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR1136323.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7398..7691
                     /gene="ybfE"
                     /locus_tag="ACHUQ7_003017"
     CDS             7398..7691
                     /gene="ybfE"
                     /locus_tag="ACHUQ7_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFR1136324.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7831..8361
                     /gene="fldA"
                     /locus_tag="ACHUQ7_003018"
     CDS             7831..8361
                     /gene="fldA"
                     /locus_tag="ACHUQ7_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFR1136325.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8571..8657
                     /gene="uof"
                     /locus_tag="ACHUQ7_003019"
     CDS             8571..8657
                     /gene="uof"
                     /locus_tag="ACHUQ7_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFR1136326.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8650..9096
                     /gene="fur"
                     /locus_tag="ACHUQ7_003020"
     CDS             8650..9096
                     /gene="fur"
                     /locus_tag="ACHUQ7_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFR1136327.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9180..9506)
                     /gene="chiQ"
                     /locus_tag="ACHUQ7_003021"
     CDS             complement(9180..9506)
                     /gene="chiQ"
                     /locus_tag="ACHUQ7_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFR1136328.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9543..10949)
                     /gene="chiP"
                     /locus_tag="ACHUQ7_003022"
     CDS             complement(9543..10949)
                     /gene="chiP"
                     /locus_tag="ACHUQ7_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HFR1136329.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWIKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFSAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
BASE COUNT         2739 a         2767 c         2803 g         2942 t
ORIGIN      
        1 atgatttatt atctggggta tgatcgtttt ttgttgatct tcttcacaga ttatagccat
       61 ttcatggata gaataactct accttcaact gacacagcaa gcggtaaagg taaatggaaa
      121 ataataaccg cttaatgcct catataaggc ggacaaccca tatcatgaag tttacccatc
      181 gtaatagttt tgactttcat ttctttaatg cccgctagtc ttctgactaa agggcacccc
      241 aacgtacagg tctccctgac tttaagcatt acaggttaat acctgtattc ctcggtgctc
      301 atatactgct aaccctttta aactctaaat aattcgagtc gcagcacttg caacttgagg
      361 tatgacgagt atagccagtt accgggctgg tctgggttat tgcatctgca aaaagcaaac
      421 tactgattta tttatcagcg gtggagcttt gctttttttc cgacgtgatc gatttctcct
      481 ttgagaaatt gaggacctgc tattacctga aataaagaga tgaataaaat gtcagaatta
      541 aaaattgccg ttagccgttc ttgcccggat tgtttttcca ctcatcgtgc atgcgtgaat
      601 attgacgaaa gtaattatat tgacgttgcc gccattattt tatcagtcaa tgatgttgaa
      661 cgtggaaaac tcgataaaat agacgctact ggctatggca ttcctgtttt tattgcaacg
      721 gaaaatgaag aacgtgtacc agcagaatat ctgccacgta tttctggtgt cttcgagcat
      781 tgcgaatcac ggaaagagtt ttatggacgt cagttagaaa ccgctgctag ccattatgaa
      841 acgcaactgc gcccgccctt cttccgcgca ctggtcgatt atgtcaatca gggcaatagc
      901 gcttttgact gcccgggtca tcagggaggt gagtttttcc gtcgtcatcc ggcagggaat
      961 cagttcgtgg agtacttcgg agaaacgctg ttccgttccg acttatgcaa cgcagacgta
     1021 gcaatgggcg atctgctgat tcacgaaggc gcgccatgca ttgcacagca acatgcggca
     1081 aaagtgttta atgccgataa aacatacttc gttctaaatg gcacttcatc ttctaacaaa
     1141 gtggttttaa acgccctgct aacaccgggt gatctggtgc tgtttgatcg caataaccac
     1201 aaatctaacc accacggagc gttgctacag gctggtgcaa caccggttta tctggaaacg
     1261 gcacgtaacc cgtatggctt tatcggtggc attgatgcgc actgttttga agaaagttac
     1321 ctgcgtgagc tgatcgcgga agtcgcaccg cagcgggcaa aagaggctcg tcctttccgc
     1381 ctcgctgtga ttcagttagg cacctacgac ggtacgattt ataacgcccg ccaggtggtg
     1441 gataaaattg gtcatctgtg tgactacatt ctgtttgact ccgcatgggt tggctatgag
     1501 caatttatcc cgatgatggc tgactgttcg ccgctgttgc tggatcttaa tgagaacgat
     1561 ccgggtattc tagttacgca atccgtacat aaacagcagg ctggtttttc tcaaacttca
     1621 caaatccata aaaaagacag ccacatcaaa gggcaacagc gttatgtacc gcacaaacgc
     1681 atgaacaacg cctttatgat gcacgcctcc accagcccgt tctatccgct gtttgccgca
     1741 ctggatatca acgccaaaat gcatgaaggt gtcagcggtc gtaatatgtg gatggattgt
     1801 gtggtaaatg gcattaatgc ccgcaaactg atcctcgata actgtcagca tattcgtccg
     1861 ttcgtacctg aactggtgga tggtaaaccc tggcagtcgt atgaaacagc gcaaattgcg
     1921 gttgatctgc gcttcttcca gtttgtacca ggggaacact ggcattcttt tgaaggctat
     1981 gcagagaatc aatactttgt cgatccttgc aaactgttgc tgacaacccc aggtattgat
     2041 gcacgtaacg gcgaatatga agcgttcggt gtacccgcga cgattcttgc taacttcctg
     2101 cgcgaaaatg gcgtagtgcc ggaaaaatgc gatcttaacg ccatcctctt cctgctgact
     2161 ccggcagaag atatggccaa acttcagcaa cttgtgccct gctggtacgc ttcgaaaaac
     2221 tgcttgagtc cgacgcgcca ttagcagaag tgctgccttc catctacaaa cagcatgaag
     2281 agcgctacgc cggttatacc ctgcgtcagt tgtgccagga aatgcatgat ttgtatgccc
     2341 gccacaacgt gaaacaactg caaaaagaga tgttccgcaa agcgcacttc ccacacgtca
     2401 gcatgaatcc gcaagaagcc aactacgcct atttacgcgg tgaagtggag ctggttcgtc
     2461 taccggatgc cgaaggtcgt atcgctgccg aaggggcgct tccttatcct ccgggtgtgc
     2521 tgtgtgttgt tccgggtgaa atctggggtg gtgctgtttt gcgttacttc agcgctctgg
     2581 aagaagggat caacctgctg ccaggttttg caccggagct gcagggtgtc tatatcgaag
     2641 aacatgatgg tcgtaagcaa gtttggtgct atgtcatcaa gcctcgtgat gcgcaaagca
     2701 ccctgttgaa aggggaaaaa ttatgagtca ggctaaatcg aacaaaatgg gcgtcgttca
     2761 gttaaccata ctgacgatgg tcaacatgat gggctccggt atcatcatgc tgccgacaaa
     2821 gcttgccgaa gttgggacaa tttcaattat ctcctggctg gtgacagccg taggctcaat
     2881 ggcactggca tgggcattcg caaaatgcgg tatgttcagc cgtaaatcag gtggtatggg
     2941 cggttatgcc gagtatgctt tcggtaaatc cggtaacttt attgcgaact atacctacgg
     3001 cgtctcactg ctgattgcta acgtcgcgat tgctatttcg gcggttggtt acggcaccga
     3061 actgctcggc gcgagtttgt cgccagtgca gattggtctt gcgaccatcg gggtgctgtg
     3121 gatttgtacc gtggctaact ttggtggtgc gcgcattacc gggcaaatca gtagcattac
     3181 cgtgtggggg ggcattattc cggtggtagg cctgtgcatt atcggctggt tctggtttag
     3241 cccgacgctt tacgttgatt cctggaatcc gcatcatgca ccgttcttca gtgcagtagg
     3301 ttcttccatc gctatgacgc tgtgggcttt tcttggtctg gagtctgcgt gtgcgaatac
     3361 tgatgtagtg gaaaacccgg aacgtaatgt gccaatcgcg gtactcggcg gtacgttagg
     3421 tgcggcggtg atttatatcg tctccaccaa cgtgattgca gggattgtgc ctaatatgga
     3481 gctggcaaat tcaacggcac catttggcct ggccttcgca cagatgttta caccagaagt
     3541 gggtaaagtc attatggcgc tgatggtgat gtcctgctgc ggttcgctgc ttggctggca
     3601 gttcaccatt gcccaggtgt ttaaatcttc atctgatgaa ggctacttcc ctaaaatttt
     3661 ctcccgtgta accaaagtgg atgcaccggt gcagggaatg ttgaccattg tgattatcca
     3721 gagcgggttg gcgctgatga cgattagccc gtcgctaaac agtcagttca acgtgctggt
     3781 taacctggcc gtggtgacca atatcattcc gtatattctg tcaatggcgg cattagtcat
     3841 tattcagaag gtcgctaatg tgcccccctc aaaagcgaaa gttgcaaact ttgttgcttt
     3901 tgttggcgcg atgtatagct tttatgcgct gtactcatcc ggggaagaag ccatgctgta
     3961 cggttccatc gtgaccttcc ttggttggac actgtatggt ctggtatcac cacgctttga
     4021 actgaaaaat aaacacggtt aaaaaagcaa agtaaaaaag ggcgatcttg cgaccgctct
     4081 ttttttatta aatgtgttta cgcgtttttc agaacttcgc taacaatctc aaccgcttct
     4141 ttctcaatct gcttgcgatg ttcttcaccg aggaagcttt cgcagtagat cttatatgcg
     4201 tcttccgtgc ctgacggacg cgcggcgaac cagccgttgt cagtcatcac tttcagaccg
     4261 ccaatagaag caccgttgcc cggagcagca gtcaggcgcg cggtgatcgg gtcacctgcc
     4321 agggtgctgg cgctcaccat ttccggagac agcttagaca gcgccgcttt ttgtgcggaa
     4381 gtcgcagctg cctgcaaacg gttgtagctc ggcgcaccaa agcgttttgc cagttcgttg
     4441 tagtgttcct gcgggttctt accggtgaca gcggtgattt ccgccgccag cagacacata
     4501 atgatgccgt ctttgtcggt ggaccacggc gtgccgtcga aacgcaggaa ggaagcccct
     4561 gcgctctctt cgccgccaaa gccgaagctg ccgtcgaaca gaccatcaac aaaccatttg
     4621 aaacctaccg gtacttctac cagcttacgg cccaagtcat tgaccacacg gtcgatcatc
     4681 gcagaagaaa ccagcgtttt accgacagca acatctttgc cccactgcgg acgatgctgg
     4741 aacaggtagt tgatcgccac cgccaggtag tggttcggat tcatcaaacc tgccggagtg
     4801 acgataccgt gacggtcata atccgggtcg ttagcaaacg ccagatcgaa cttatcacgc
     4861 agtgccagca gacccgccat cgcacactcg gaggagcagt ccatacggat cgcaccgtct
     4921 ttatcgaggt gcataaagcg gaaggtttga tcgacctgat cgttaacgat ggtcaggttg
     4981 aggttgtaat actcgccaat gcgcttccag tattcgatac cggaaccgcc caacggatca
     5041 acgcccagcg tcaggcccgc tttctgaatc gccgccatat caacgatatc ggccagccct
     5101 tccacgaacg gctgcaccag atcctgctct ttcacatagc cggatgccat cgcttcgtcg
     5161 agggagatac gcttcacgcc tttcaggcca tcggccatca gtgcgttggc cttgtcttcc
     5221 accactttgg tgacgttagt atcagccggg ccaccatttg gcggattgta cttgatacca
     5281 ccatcttccg gcgggttatg ggacggtgta atcacgatac cgtctgccag cgggccacct
     5341 tttttattgt gaaccaggat ggcattggaa acggcaggcg tcggagtgaa gccattgttt
     5401 tcctgcacaa tgacatcaac gccgttcgct gccagcactt ccagtacgga aatgaacgca
     5461 ggctcggaca gggcgtgagt atctttaccc acatagcaag ggccagtgat gccgtttttc
     5521 gcacgttctt cagcaattgc ctgagcgatc gccagaatgt gcggctcgtt aaagctgtgg
     5581 cgcgctgcac tgccacggtg accggaagta ccgaatttca ccgcgtgctc cgcattccct
     5641 gcttctggtt tcagtacata atattgcgcc gtcagttggg cgacgttaat caaatcactc
     5701 tgttgtgcag gttggcctgc acgattgtgg attgccattg ctttgtcctt tgtctgcaac
     5761 gttttagata gttccgcaaa ccttctcaat caattccgcc gggaattgca tcgactgcat
     5821 gatgtgttcg atcatgctgc atttacggcc ggtgttggtg ttggtgatca cccaatacgg
     5881 cgtgcctggc acatgtttcg gcttggtctg attaccattt ttcagcagcg tttgttcatc
     5941 tgccgcaaag tacacgcgtg tacgaccgtg caacgattcc gttgcttcgg caaacgcctg
     6001 ggcgtcaaga gaatatagtg tagacaacag cagcataaag cgattgaccg ctcgcttttg
     6061 ctctgcgtat tcgtccgaaa gcagaagttc acgcattgcg cgaaccttgt ctttaatcgt
     6121 tttgaccggc ttcgcttcga cgatagcagg tgacgcaacg cgaacctctt tcgtcaccgg
     6181 agcagcaggc tgtgatgcgg cggaaaattt caacatacgc cgtaaaatgt cggatgcgct
     6241 ctcgccgata tgcttagtgt ggctggcaat atagctgtag agttcatcat caacttcaat
     6301 cgttttcatc ttaatccagt gcagtgtctt agctgaatac aagtcgccaa gagtatatga
     6361 ataaatcccg gcagcggata gcgtcaagcc tggcttgcag aaaaactcgg aataaacctt
     6421 atttgttgca gccctgtggc acaatggtca acataatacc ctaacccgac aacgcgaaaa
     6481 aaagaacttt gccatgaaat taaatatccg cgcgcaaact gcacaaaacc agcacaataa
     6541 ttctcccatc gttcttgtcc atggtctgtt tggtagcctc gacaaccttg gcgtactggc
     6601 tcgcgatctg gtaaacgatc acaatatcat ccaggttgat atgcgtaacc acggtctttc
     6661 accgagagag ccggtaatga attacccggc gatggcgcag gatcttgttg ataccctgga
     6721 tgcactgcag atcgacaaag caacatttat cggtcactcc atgggcggta aagcggtaat
     6781 ggcacttact gcactggccc ccgatcgcat cgataaactg gtggcgatcg atatcgcacc
     6841 ggtcgactat cacgtacgcc gtcatgatga gattttcgca gctatcaacg cggtcagtga
     6901 atcggacgca caaactcgcc agcaagcagc agcaataatg cgccagcatc ttaatgaaga
     6961 aggggtgatt cagtttctgt tgaaatcttt tgttgacggg gagtggcgct ttaacgtgcc
     7021 ggtattgtgg gatcagtatc cgcatattgt gggttgggag aaaatcccgg catgggatca
     7081 ccctgccctg tttatccctg gcggcaattc tccgtatgtt agcgagcagt accgtgatga
     7141 tttactggca caatttccac aggcacgggc gcatgtgatt gcaggcgcgg gtcactgggt
     7201 ccatgctgaa aaaccggatg cggtattacg cgctatccgt cgctatctca atgattaacg
     7261 caactgatta aaaacccagc gtccgcgtcg ctttccgcgc gtgggcgttg gcgtgactca
     7321 tcgcgctgat gtattatggc gcgctatcca tccgggcaga ggcctggctg attgtttccc
     7381 ccgaagtcac caagatcatg gccaaagaac aaacggaccg tacgacatta gatctgttcg
     7441 cgcacgagcg tcgaccggga cgaccgaaaa ctaatccgct ttcgcgcgat gaacagctgc
     7501 gtattaataa acgcaaccag ctaaaacgcg acaaagtacg tggccttaag cgtgtcgaac
     7561 tgaagctgaa cgcggaagct gtcgaggcgc tgaacgagct ggcggaatcg cgtaatatga
     7621 gccgtagcga actgatcgaa gagatgttaa tgcagcaact ggcggcgctg cgtagtcagg
     7681 gcatcgttta aatttccact ttcatgtagc acagtgtgca gtcctgctcg tttgctgatt
     7741 ccgcaagact gcctgttctg ctatgattgc ctttatccgt gggcaatttt ccacccccat
     7801 ttcaataagt ttcaagaggt tatttcactc atggctatca ctggcatctt tttcggcagc
     7861 gacaccggta ataccgaaaa tatcgcaaaa atgattcaaa aacagcttgg taaagacgtt
     7921 gccgatgtcc atgacattgc aaaaagcagc aaagaagatc tggaagctta cgacattctg
     7981 ctgctgggca tcccaacctg gtattacggc gaagcgcagt gtgactggga tgacttcttc
     8041 ccgactctcg aagagattga tttcaatggt aaactggttg cgctgtttgg ttgtggtgac
     8101 caggaagatt acgccgaata tttctgcgac gcattgggca ccatccgcga catcattgaa
     8161 ccgcgcggtg caaccatcgt tggtcactgg ccaactgcgg gctatcattt cgaagcatca
     8221 aaaggtctgg cagatgacga ccactttgtc ggtctggcta tcgacgaaga ccgtcagccg
     8281 gaactgaccg ctgaacgtgt agaaaaatgg gttaaacaga tttctgaaga gttgcatctc
     8341 gacgaaattc ttaatgcctg atgtgatgcg gcgtagactc atgtctacgc cgtattaata
     8401 gataatgcca atcaaaataa ttgctacaaa tttgtaactt ttgctgttgt acctatacaa
     8461 tgtcccagtg ttcaagtggc cttgccgttg taaatgtaag ctgtgccacg tttttattaa
     8521 caatatttgc cagggacttg tggttttcat ttaggcgtgg caattctata atgatacgca
     8581 ttatctcaag agcaaattct gtcacttctt ctaatgaagt gaaccgctta gtaacaggac
     8641 agattccgca tgactgataa caataccgcc ctaaagaaag ctggcctgaa agtaacgctt
     8701 cctcgtttaa aaatcctgga agttcttcag gagccggaca accatcacgt cagtgcggaa
     8761 gatttataca aacgtctgat cgatatgggt gaagaaattg gtctggctac ggtatatcgc
     8821 gtactgaacc agtttgacga cgctggtatc gtcacccgcc acaattttga aggcggtaaa
     8881 tccgtatttg aactgacaca gcaacatcac cacgatcacc tgatttgcct cgactgcggc
     8941 aaggttatcg aatttagtga tgattccatc gaagcgcgtc agcgtgaaat tgccgcaaaa
     9001 catggcattc gcctgactaa ccacagtctc tatctttacg gtcactgtgc cgaaggcgat
     9061 tgccgcgaag atgagcacgc gcacgaaggc aaataagcca gcctgaacga gaaaagccaa
     9121 cctgcgggtt ggctttttta tacaagggaa ataagaaaag cgagagttac agctctcact
     9181 tatttgttat tactgcgtat ttcctgccag accttatcac aatcggcttt caccgcctga
     9241 tcatttccgg tttgcgttgc acgcagacac tgctgataat ccagtacgcg tacactttcc
     9301 tggtcggcaa actgctgatg ttgtttctct ttcttcagca cgtttaacac gctctggcag
     9361 gcttcaattt tttctggcga accttgcgcg gtgttgatac aggcgctgta tgcctctttc
     9421 agacggctgt cttcctgtgg tgcggttgat tgcgcacaag ctaccagccc cgatgccatc
     9481 atggcgatga gaatcagttt tttcattgaa tgcggcaggc acaaaacctg ccgcgtcggg
     9541 catcagaaga tggtgaatgg tgcgattacc ataaatttca cgtcacgctc atcctggaag
     9601 atgttaccgt aaccgccgcc ccagcttggg atgtcggagt ggttgtcgta ttcggtgaag
     9661 tgcagtttga acatcgtgcc tttggcgcga ccgtcctgaa tggtgtagac cgcatccagg
     9721 ctgtaggcag actcttcaat agtccggttt ttgtcgtagt acgcatccgg attgctctgc
     9781 caggtcgcag gtttagcatc ccatgcgtaa acgtaggaag cgccgatggc gaagcctgga
     9841 agattccagt ttttcaggtc atacatcgca ctgaagaaga ccgctttttc gccgttagcg
     9901 ttgaagtcgg aacggttatc ccaccagata tccaggcggc cgttggagga agcgtaggtt
     9961 ggggtcatac gttgcaggaa gtatccctgc tgactgtcag ccttaatcca ggtgccttcg
    10021 aggcgcaaat ccactacgtc agccgcccgg taaccaaagg tcaacgcttg cagccaggcg
    10081 gtgccgtcat aaagatcgtt gacgctgcga tcgtcaactt tatcgcgggt accatagaac
    10141 tggtagctgg tggttaacgg gctaccggcg atatcaaatt tgtagctggc tttggcaaaa
    10201 tattgatcga tatacccttc cgcctgacca aatgccgctt ccagtacgaa gttatttttg
    10261 aagtcgtatt tcgcaccgat ggagtgcaga taatcaactt tggtggtttt atcgttctga
    10321 taaaactcat ccatttccag atgccacggc gctttgtatt cgttggtcca catgtaggag
    10381 aaactcaagg caccagcatc gccgtaatca aaattcgccc cggcttccgc cccctgataa
    10441 gtacccggca taaagctcca gtgcggcgct aacagcgttt gaccggttgg ctgaatgtaa
    10501 cctgccctcg cccaaaccgg accgtattta aatttggccg cagctttata caggcttata
    10561 ccgcttttgt cgccggacca gtcttcgtca taggctttat tactttttga aaacgcgatt
    10621 tcgttcgggt gggagctgtc gccgttttcc gccatttcaa tcgccgtaaa cgcagcaata
    10681 tcaaggccga acatatcagc agcataaccg gactgaaaat cgaggttggc attccaggtg
    10741 gagtgagaaa ggttggtttt gtatttgtcg ccgtcggtaa catctttacg gtcgcgttca
    10801 cgctgccagt aatagatacc gccggttaag gttgaatcgt cgatgaatcc ttcggcgcga
    10861 gcctccggca ttgccataaa gcccgacatt gctgtaacac cggcaatagc cagcgccagc
    10921 gtactacgtt tgccactaaa cgtacgcatg ggttaatcct ctttgacgta taaattgctg
    10981 caccaaaggt gaacagcgaa aaaaaataaa aattaaaaaa gtagcgttga gaaaagtctc
    11041 gctgactaca ggaacagact atttttcgcg acgcgaatta tcaatctctt tctgtaacca
    11101 gaatgtaaga acatgagcaa ccgcacatat tttgatgatt tttgttacaa ggcttaagtt
    11161 tgcggttgaa aggcttgcag aagaataaaa acgtgaaatt ccagcaagat gaaagcggtt
    11221 acattttatt taattatgtt ttgtgatttt a
//
LOCUS       DAZPCS010000121        11066 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZPCS010000121 DAZPCS010000000
VERSION     DAZPCS010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11066
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.121"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            255..1396
                     /gene="yghO"
                     /locus_tag="ACHUQ7_003023"
                     /pseudo
     CDS             255..1396
                     /gene="yghO"
                     /locus_tag="ACHUQ7_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YghO"
     gene            complement(1520..2290)
                     /gene="glcC"
                     /locus_tag="ACHUQ7_003024"
     CDS             complement(1520..2290)
                     /gene="glcC"
                     /locus_tag="ACHUQ7_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HFR1136330.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSHVARLNREQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENHKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNKVGM"
     gene            2541..4040
                     /gene="glcD"
                     /locus_tag="ACHUQ7_003025"
                     /pseudo
     CDS             2541..4040
                     /gene="glcD"
                     /locus_tag="ACHUQ7_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009339 - glycolate oxidase complex
                     [Evidence IEA];
                     GO_function: GO:0003973 - (S)-2-hydroxy-acid oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
     gene            4040..5092
                     /gene="glcE"
                     /locus_tag="ACHUQ7_003026"
     CDS             4040..5092
                     /gene="glcE"
                     /locus_tag="ACHUQ7_003026"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HFR1136331.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVVGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            5103..6322
                     /gene="glcF"
                     /locus_tag="ACHUQ7_003027"
                     /pseudo
     CDS             5103..6322
                     /gene="glcF"
                     /locus_tag="ACHUQ7_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
     gene            6327..6731
                     /locus_tag="ACHUQ7_003028"
     CDS             6327..6731
                     /locus_tag="ACHUQ7_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG family protein"
                     /protein_id="HFR1136332.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKVAAAVLAK"
     gene            6753..8924
                     /gene="glcB"
                     /locus_tag="ACHUQ7_003029"
     CDS             6753..8924
                     /gene="glcB"
                     /locus_tag="ACHUQ7_003029"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HFR1136333.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFFRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHHTNVQSVQANIAQTEFS
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            9279..10865
                     /locus_tag="ACHUQ7_003030"
     CDS             9279..10865
                     /locus_tag="ACHUQ7_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008028 - monocarboxylic acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015718 - monocarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate permease LctP family transporter"
                     /protein_id="HFR1136334.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGATVAFGALGVPILVAGQVTGIDPF
                     HIGAMAGRQLPFLSVLVPFWLVAMMDGWKGVKETWPAALVAGGSFAVTQFFTSNYIGP
                     ELPDITSALVSIVSLALFLKVWRPKNTETAISMGQSAGAMVVNKPSSGGPVPSEYSLG
                     QIIRAWSPFLILTVLVTIWTMKPFKALFAPGGAFYSLVINFQIPHLHQQVLKAAPIVA
                     QPTPMDAVFKFDPLSAGGTAIFIAAIISIFILGVGIKKGIGVFAEMLISLKWPILSIG
                     MVLAFAFVTNYSGMSTTLALVLAGTGVMFPFFSPFLGWLGVFLTGSDTSSNALFGSLQ
                     STTAQQINVSDTLLVAANTSGGVTGKMISPQSIAVACAATGMVGRESELFRYTVKHSL
                     IFASVITLLQAYVFTGMLVS"
BASE COUNT         2589 a         2744 c         3084 g         2649 t
ORIGIN      
        1 aaataatgag tttaagtttt ttgtttttat tatgaatgat atttaattta tgaatatatt
       61 aagaagaagc cttgtccggt cgccttaaat tgacaaaagg cattgaggcg tttctgtgta
      121 gcctctgacc ttatcataat cattaattaa tactatttat tcgtcgtttg tattcattga
      181 gttgattatt aagatgccgc tcgttgctgt tgcaacctgt ttttcaccat taattatttg
      241 ttatccacag agatatggaa tgcgatctgc tgatgattaa aattgaaaaa gtaattaata
      301 aaaatgacct taaggcattt atcgcttttc cgtcatcact ttatcccgac gatccaaact
      361 ggataccccc tttattcatt gaacgcaacg agcatttgtc tgcgaaaaat ccggggacgg
      421 accatatcat ctggcaggcg tgggtggcaa aaaaagcggg gcagatagtt gggcgtatta
      481 ccgcgcaaat agataccttg caccgtgagc gttacggtga agataccggt catttcggca
      541 tgattgacgc cattgatgat ccgcaggatt ttgccgcgtt gtttggcgca gcggaagcgt
      601 ggttgaagtc acaaggtgca agtaagatca gcgggccttt cagcctgaat atcaatcagg
      661 aaagcggatt actgattgaa ggttttgaca caccaccctg tgcgatgatg ccacacggca
      721 aaccgtggta tgccgcgcat attgaacaac tgggttatca caaaggcatt gatttactgg
      781 cgtggtggat gcagcgaacc gatctcactt tctctccggc gctaaaaaaa ctgatggatc
      841 aggtgcgcaa aaaggtgacc attcgctgca tcaatcgtca gcggtttgcc gaagagatgc
      901 agatcctgcg tgagattttc aactctggct ggcagcacaa ctggggattt gtgccgttta
      961 ccgaacatga attcgcgacc atgggcgacc aacttaaata tctggtgccg gacgatatga
     1021 tctacatcgc tgagattgat tctgcaccct gcgcgtttat tgtggcttgc ctaacatcaa
     1081 cgaggcgatt gccgatctga acggatcgct ctttcccttc ggctgggcaa aattgctgtg
     1141 gcgtttgaaa gtcagcggtg tgcgaactgc gcgagtaccg ctgatgggcg tgcgcgacga
     1201 gtatcagttc agccgcatcg gcccggtgat tgcgctgtta ttgattgaag ccttacgcga
     1261 tccgtttgcc cgccggaaga tcgatgcgct ggagatgtca tggatcctcg aaaccaatac
     1321 aggcatgaat aacatgctgg aacgcattgg ggcggagccg tacaagcgtt accgattgta
     1381 tgaaaagcaa atttaatacc agatcctgtt tagcgcaggg gcggtcattc gggagagggt
     1441 atagatgaac agatttaaca ccagatcctg ttaagtgcag gggcggtccc ctcgccttgg
     1501 ggatagggta aaactctgct cacattccca ccttgttcat ctccagcggc accgccgagc
     1561 gaatcaaatg gtggccttcc agctcgatat cgtggagatt ctttttcacg gtccgtacat
     1621 gatcgcgcgc tgcgcgctga gcgacgtgcg gcagccgctg caacaccgcg ttgtagatcc
     1681 gcgcatgctg gcgatcgatc tgcttttttt gctgtggtcg atggtagaga ttatttacgc
     1741 tggcaaacac tgaattaaac atcagatcgg tcaatgattg cagcgtaaac accagcacct
     1801 gattgtgaga agcctgacaa atggcgagat ggaaagcgtg atccaactgc gcatgttcga
     1861 tcagcgaaat ctctttgtgg ttctcactgg cggcgagcat tttttcataa cagcgggtta
     1921 tcacaacaaa atcagcctgc gttcccagcg ttgccgccag ccttgccgat tcgccctcca
     1981 gtaatgcgcg aacgtcgagc agatcgtaca gcgttcgcgg ctgcgtactg aacagatgga
     2041 tcagcgggct ggtgtcctgc tcccgattaa gccgtgcgac atgagaatcg cgaccctgcg
     2101 ccgtttcaat aatcccgcgc ccgcgcagca cggtcagccc ttcacgcagt gcggagcgtg
     2161 agaagccgag cttttcacac agtcgacgtt ccgagggaag cggctgaccg accttcagta
     2221 cgccgtcgat aattaaccgt tcgatactct ctgcaaccac ttcgcaaata gggcgacgtt
     2281 catctttcat tcccggacct cgtgcacagg taggaccaat ttttctgttc ttaactcgca
     2341 aaacacgcac atcacgcaag tgattatgtt acatcaattt aacattaagt taaccaagac
     2401 aaggtcacag agctggaaaa aaaatggtct gaccggtagc taaagagata gacgaaaacg
     2461 aaaagcccgc ttaataactg ttcacagaag cagcgcgcaa aaatcagctg ccacacaaca
     2521 caacaaagcg aagcctactc atgagcatct tgtacgaaga gcgtcttgat ggcgctttac
     2581 ccgatgtcga ccgcacatcg gtactgatgg cactgcgtga gcatgtccct ggacttgaga
     2641 tcctgcatac cgatgaggag atcattcctt acgagtgtga cgggttgagc gcgtatcgca
     2701 cgcgtccatt actggttgtt ctgcctaagc aaatggaaca ggtgacagcg attctggctg
     2761 tctgccatcg cctgcgtgta ccggtggtga cccgtggtgc aggcaccggg ctttctggtg
     2821 gcgcgctgcc gctggaaaaa ggtgtgttgt tggtgatggc gcgctttaaa gagagcctcg
     2881 acattaaccc cgttggtcgc cgcgcgcgcg tgcagccggg cgtatgtaac ctggcgatct
     2941 cctaggccgt tgcgccgcat aatctctatt acgcgccgga tccttcctca caaatcgcct
     3001 gttcaattgg cggcaatgtg gcggaaaatg ccggtggcgt ccactgcctg aaatatggtc
     3061 tgaccgtaca taacctgctg aaaattgaag tgcaaacgct ggacggcgag gcgctgacgc
     3121 tgggatcgga cgcgctggat tcacctggtt ttgacctgct ggcgctgttc accggatcgg
     3181 aaggtatgct cggcgtgacc accgaagtga cggtaaaact gctgccgaag ccgcccgtgg
     3241 cgcgggttct gttagccagc tttgactcgg tagaaaaagc cggacttgcg gttggtgaca
     3301 tcatcgccaa tggcattatc cccggcgggc tggagatgat ggataacctg tcgatccgcg
     3361 cggcggaaga ttttattcat gccggttatc ccgtcgacgc cgaagcgatt ttgttatgcg
     3421 aactggacgg cgtggagtct gacgtacagg aagactgcga gcgggttaac tacatcttgt
     3481 tgaaagcggg cgcgactgac gtccgtttgg cacaggacga agcagagcgc gtacgtttct
     3541 gggccggtcg caaaaatgcg ttcccggcgg taggacgtat ctccccggat tactactgca
     3601 tggatggcac catcccgcgt cgcgccctgc ctggcgtact ggaaggcatt gcccgtttat
     3661 cgcagcaata tgatttacgt gttgccaacg tctttcatgc cggagatggc aacatgcacc
     3721 cgttaatcct tttcgatgcc aacgaacctg gtgaatttgc ccgcgcggaa gagctgggcg
     3781 ggaagatcct cgaactctgc gttgaagttg gcggcagcat cagtggggaa catggcatcg
     3841 ggcgagaaaa aatcaatcaa atgtgcgccc agttcaacag cgatgaaatc acgaccttcc
     3901 atgcggtcaa ggcggcgttt gaccccgatg gtttgctgaa ccctgggaaa aacattccca
     3961 cgctacaccg ctgtgctgaa tttggtgcca tgcatgtgca tcacggtcat ttaccttttc
     4021 ctgaactgga gcgcttttga tgctacgcga gtgtgattac agtcaggcgc tgctggagca
     4081 ggtgaatcag gcaattagcg ataaaacgcc gctggtgatt cagggcagca atagcaaagc
     4141 ctttttaggt cgcccagtca ccgggcaaac gctggatgtg cgttgtcatc gcggcattgt
     4201 taattacgac ccgaccgagc tggtgataac cgctcgtgcc ggaacgccgc tggtggcgat
     4261 tgaagcggcg ctggagagcg ccgggcaaat gcttccctgc gagccgccgc attatggtga
     4321 agaagccacc tggggcggga tggtcgcctg cgggctggcg gggccgcgtc gcccgtggag
     4381 cggttcggtc cgcgattttg tcctcggcac gcgcatcatt accggcactg gaaaacatct
     4441 gcgttttggt ggcgaagtga tgaaaaacgt tgccggatac gatctctcac ggttaatggc
     4501 cggaagctac ggttgtcttg gcgtgctcac tgaaatctca atgaaagtgt taccgcgacc
     4561 gcgtgcctct ctgagcctgc gtcgggaaat cagcctgcaa gaagccatga gtgaaatcgc
     4621 cgagtggcaa ctccagccat tacccattag tggcttatgt tacttcgaca atgcgttgtg
     4681 gatccgcctt gagggcggcg aaggatcggt aaaagcagcg cgtgaactgc tgggtggcga
     4741 agaggttgtg ggtcagttct ggcagcaatt gcgtgaacaa caactgccgt tcttctcgtt
     4801 accaggtacc ttatggcgca tttcattacc cagcgatgcg ccgatgatgg atttacccgg
     4861 cgagcaactg atcgactggg gcggggcgtt acgctggctg aaatcgacag ccgatgacaa
     4921 tcaaatccat cgcatcgccc gcaacgctgg cggtcatgcg acccgcttta gtgccggaga
     4981 tggtggcttt gccccgctat cggctccttt attccgctat caccagcagc ttaaacagca
     5041 gctcgaccct tgcggcgtgt ttaaccccgg tcgcatgtac gcggaacttt gaggagcagg
     5101 ctatgcaaac ccgattaacc gaagagatgc ggcagaacgc gcgctggaag ccgacagcat
     5161 cctgtgcgcc tgtgttcact gcggattttg taccgcaacc tgcccaacct atcagcttct
     5221 gggcgatgaa ctggacgggc cgcgcgggcg catctatctg attaaacagg tgctggaagg
     5281 caacgaagtc acgcttaaaa cacaggagca tctcgatcgc tgcctcactt gccgcaattg
     5341 tgaaaccacc tgtccttctg gtgtgcgcta tcacaatttg ctggatatcg ggcgtgatat
     5401 tgtcgagcag aaagtgaaac gcccactgcc ggagcgaatg ctgcgcgaag gattgcgcca
     5461 ggtagtgccg cgtccggcgg tcttccgtgc gctgacgcag gtagggctgg tgctgcgacc
     5521 gtttttaccg gaacaggtca gagcaaaact gcctgctgaa acggtgaaag ctaaaccgcg
     5581 tccgccgctg cgccataagc gtcgggtttt aatgctggaa ggctgcgccc agcctacgct
     5641 ttcgcccaac accaacgcgg caactgcgcg agtgctggat cgtctgggga tcagcgtcat
     5701 gtcagctaac gaagcaggct gttgtggcgc ggtggactat catcttaatg cgcaggagaa
     5761 agggttggca cgggcgcgca ataatattga tgcctggtgg cccgcgattg aagcaggtgc
     5821 cgaggcaatt ttgcaaaccg ccagcggttg cggcgcgttc gtcaaagagt atgggcagat
     5881 gctgaaaaac gatgcgttat atgccgataa agcgcgtcag gtcagtgaac tggcggtcga
     5941 tttagtcgaa cttctgcgcg aggaaccgct ggaaaaactg gcaattcgcg gcgataaaaa
     6001 actggccttc cactgcccgt gtaccctaca acatgcgcaa aagctgaacg gcgaagtgga
     6061 aaaagtgtta cttcgtcttg gatttacctt aacggacgtt cccgacagcc atctgtgctg
     6121 cggttcagcg ggaacatatg cgttaacgca tcccgatctg gcacgccagc tgcgggataa
     6181 caaaatgaat gcgctggaaa gcggcaaacc ggaaatgatc gtcaccgcca acattggttg
     6241 ccagacgcat ctggcgagcg ccggtcgtac ctctgtgcgt cactggattg aaattgtaga
     6301 acaagccctt gaaaaggaat aaaaaaatga aaactaaagt cattcttagc cagcaaatgg
     6361 cgagtgcaat tattgccgca ggtcaggaag aggcgcagaa aaataactgg tctgtttcca
     6421 ttgctgttgc cgatgacggc ggtcatctgc tggcgttaag tcgcatggac gattgcgcgc
     6481 cgattgcggc ttacatctcc caggagaaag cgcgtaccgc cgcgctgggg cgtcgtgaaa
     6541 ctaagggcta tgaagagatg gtgaacaacg gacgtaccgc gttcgtgact gcgccgttac
     6601 tgacgtcgct ggaaggcggc gtaccggttg ttgttgatgg gcaaattatt ggtgccgtgg
     6661 gcgtttctgg tttaaccgga gcacaggatg cgcaggtcgc gaaagtggca gcagcggtgt
     6721 tggcgaaata agcgaaaacg aggagagaaa caatgagtca aaccataacc cagggccgtt
     6781 tacgcattga cgccaatttt aaacgttttg tggatggaga agttttgccg ggcgtggaac
     6841 tggacgctgc tgcattctgg cacaatgttg atgagatcgt tcacgatctg gcaccagaaa
     6901 atcgtcagtt gctggcagaa cgcgatcgca ttcaggcggc gcttgatgag tggcatcgca
     6961 gcaatccggg gccggtaaaa gataaagcgg cctataaatc tttctttcgt gaactgggct
     7021 acctggtgcc gcaaccggag cgcgtgacgg tggaaaccac gggcattgac agcgaaatca
     7081 ccagtcaggc ggggccacag ctggtggttc cggcaatgaa cgcccgctac gcgctgaacg
     7141 cggcgaacgc tcgctggggc tcactgtacg atgcgttata cggcagcgac atcatcccgc
     7201 aggaaggggc gatggtcagt ggctacgatc cgcagcgcgg tgagcaggtt atcgcctggg
     7261 ttcgacgttt cctcgatgaa tcactaccgc tggaaaacgg cagctatcag gatgtggtgg
     7321 cgtttaaggt ggtcgataaa caattacgca tccagttgaa aaatggtaaa gaaaccacgt
     7381 tacgtactcc agcacagttt gtcggttacc gtggcgatgc cgctgcgccg acctgcattt
     7441 tgctgaaaaa taacggcctg catattgaac tgcaaattga tgccaacggg cggattggca
     7501 aagacgatcc ggcgcacatc aacgatgtta tcgtcgaagc tgctatcagt accattctcg
     7561 actgcgaaga ttcggtcgcg gcggttgatg cggaagataa aatcctgctg taccgcaatc
     7621 tgctgggcct gatgcagggg actctgcaag agaaaatgga gaaaaacggt cggcaaatcg
     7681 tacgtaaact gaatgacgat cgtcagtaca ccgccgccga tggctctgaa atttctctgc
     7741 acggacgctc gttgctgttt atccgcaacg tgggtcattt gatgaccatt cctgtgattt
     7801 gggacagcga aggcaatgaa atcccggaag gtattcttga tggcgtcatg actggcgcga
     7861 ttgccctcta tgatttaaaa gtgcagaaaa actcgcgcac tggcagcgtc tatattgtga
     7921 aaccgaaaat gcacggtccg caggaagtgg cgttcgccaa caaactgttt acccgcattg
     7981 agacaatgct cggtatggca ccgaataccc tgaaaatggg cattatggat gaagaacgcc
     8041 ggacctcgct gaacttgcgt agctgtatcg ctcaggcgcg caaccgcgtg gcgttcatca
     8101 ataccggttt cctcgaccgt accggcgatg aaatgcattc ggtgatggaa gctggcccga
     8161 tgctgcgtaa aaaccagatg aaatcgacgc cgtggatcaa agcctacgaa cgtaataacg
     8221 tgctttccgg tctgttctgt gggctgcgcg gtaaagcgca aatcggtaaa ggcatgtggg
     8281 caatgccgga cctgatggca gacatgtaca gccagaaggg cgaccaactg cgtgccgggg
     8341 caaacacagc ctgggttccg tcaccaaccg ctgctacgct ccatgcgctg cactaccacc
     8401 ataccaacgt acagagcgtg caagccaaca ttgcccagac cgagttcagt gctgaatttg
     8461 aaccgctgct ggacgatctg ctgactattc cggtggctga aaacgctaac tggtcggcgc
     8521 aagagatcca acaagagctg gataacaacg tgcaggggat tctggggtac gtggtgcgtt
     8581 gggtggagca ggggattggt tgttcaaaag tgccggatat tcacaacgtg gcgctgatgg
     8641 aagaccgcgc aacgctgcgt atctccagcc agcatatcgc caactggtta cgtcacggta
     8701 ttctgaccaa agaacaggtg caggcgtcgc tggagaatat ggcgaaagtg gttgatcagc
     8761 aaaatgctgg cgatccggct tatcgtccga tggcggggaa tttcgctaac tcgtgtgctt
     8821 ttaaagctgc cagcgattta atcttcctcg gcgtgaaaca gccaaacggt tataccgaac
     8881 cgttattaca cgcctggcgt ttacgcgaaa aagaaagtca ttaatagcgt caaaaaagcc
     8941 ccggcaagtt ttattgtcgg ggctatgata gtaactgcgg tagtacattt atcccggaaa
     9001 taaccggagc gattatcagg gtttaacaat attaaatacc cgtagcgcat tactagcggg
     9061 cctaaaacaa caatatggac atcttaacga tactctgcgc ctgcgtgtgc ggaacagaaa
     9121 cccttacccg atatacttgt tatgccagag gtagcaattg cgttagcggc gtactgttgc
     9181 tgtaaagtat aacgcctata ttttacagtt cagtcctctt ttgcttatta caaccttaag
     9241 tatatcaagc atataaagat aataagagac tgaacaatat ggttacctgg acccaaatgt
     9301 atatgccgat gggaggactg gggctatccg ctctggtcgc cctgatcccg ataatattct
     9361 tcttcgttgc actcgcggta ttacgtctga aaggacatgt tgctggagca ataaccctta
     9421 tattatctat cctgattgcg atattcgcct ttaaaatgcc gattgatatg gcatttgctg
     9481 ctgcgggcta tggctttatt tatggattat ggccaattgc gtggattatt gtcgcggcgg
     9541 tgttcctgta taaattaacc gttgccagcg ggcagttcga tattatccgc agctcggtta
     9601 tctccatcac cgacgatcag cgtttgcagg tgttactgat tggtttctcc tttggtgcgt
     9661 tgctggaagg agctgctggc tttggtgcga cggtggcgtt tggtgcgttg ggcgtgccga
     9721 ttctggtcgc cggtcaggta acgggaatcg atccgttcca cattggcgca atggcgggac
     9781 gtcagttacc gttcctgtcg gttcttgtgc cgttctggct ggtagcaatg atggacggct
     9841 ggaaaggggt gaaagagacg tggccagcgg cgctggttgc tgggggaagc ttcgctgtca
     9901 ctcagttctt tacctctaac tatattggtc cggaactgcc ggatattact tcggcgctgg
     9961 tgagtatcgt ctcactcgct ttattcctta aagtctggcg gccgaaaaat accgaaacgg
    10021 caatcagcat ggggcaatcc gcaggtgcga tggtggtaaa taagccatct tctggcggtc
    10081 ccgtgccttc agaatatagt ctggggcaaa tcattcgtgc gtggtcaccg tttttaatct
    10141 taacggtgct ggtcaccatc tggaccatga agccgtttaa agcgttattt gctccgggcg
    10201 gcgcgtttta ttcactggtg attaatttcc agatccctca tttgcatcaa caagtgttga
    10261 aagcggcacc cattgtcgcc caaccaacgc caatggatgc ggtgtttaaa ttcgaccctc
    10321 tctcggctgg cggcaccgct atttttattg cggcgattat ctctatcttc atcctcggcg
    10381 tgggcatcaa gaaagggatt ggcgtctttg ccgaaatgct aattagtctg aagtggccga
    10441 tattgtcgat tggcatggtg ctggcattcg ccttcgtcac caactattcc ggcatgtcca
    10501 ccacgctggc gctggtactg gcaggtacag gcgtgatgtt cccgtttttc tcaccgtttc
    10561 tcggctggct gggcgtattc cttaccgggt cggacacttc ttcaaacgcc ctgtttggtt
    10621 cactgcaatc gaccacggcg cagcaaatca acgtctctga cactctgctg gtggcagcaa
    10681 ataccagcgg cggcgtaact ggcaagatga tctccccgca atctatcgcc gtggcctgcg
    10741 ccgcgacggg catggtgggc cgagaatctg aactgttccg ctacaccgtg aagcacagtc
    10801 tgatttttgc cagcgttatc accctgctgc aggcgtatgt gtttaccggg atgttagtct
    10861 cgtaaatcaa taaagccgga tgaaggtaaa tatcacccgg ctttccattt gtctgtcgtt
    10921 tatctcgcca gggaaccgtt tttacgattg gcttctttat acgtagtctc cgttttacga
    10981 tgcgaattgt ttaatattta tacaacttgg cttctgtgct ggttataaag tacccacttt
    11041 catgagatca attttagtat gttatt
//
LOCUS       DAZPCS010000122        11019 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZPCS010000122 DAZPCS010000000
VERSION     DAZPCS010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11019
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.122"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            111..1010
                     /gene="lysO"
                     /locus_tag="ACHUQ7_003031"
     CDS             111..1010
                     /gene="lysO"
                     /locus_tag="ACHUQ7_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFR1136335.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKAINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            1154..2806
                     /gene="hcp"
                     /locus_tag="ACHUQ7_003032"
                     /gene_synonym="priS"
     CDS             1154..2806
                     /gene="hcp"
                     /locus_tag="ACHUQ7_003032"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFR1136336.1"
                     /translation="MFCVQCEQTIRTPAENGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLNA"
     gene            2818..3786
                     /gene="hcr"
                     /locus_tag="ACHUQ7_003033"
     CDS             2818..3786
                     /gene="hcr"
                     /locus_tag="ACHUQ7_003033"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFR1136337.1"
                     /translation="MTMPTNQCPWRIQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLADVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            3919..5637
                     /gene="poxB"
                     /locus_tag="ACHUQ7_003034"
     CDS             3919..5637
                     /gene="poxB"
                     /locus_tag="ACHUQ7_003034"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFR1136338.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVMPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            5674..6675
                     /gene="ltaE"
                     /locus_tag="ACHUQ7_003035"
     CDS             5674..6675
                     /gene="ltaE"
                     /locus_tag="ACHUQ7_003035"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFR1136339.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALENNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            6686..8116
                     /locus_tag="ACHUQ7_003036"
     CDS             6686..8116
                     /locus_tag="ACHUQ7_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFR1136340.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQLALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            8215..9228
                     /locus_tag="ACHUQ7_003037"
     CDS             8215..9228
                     /locus_tag="ACHUQ7_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFR1136341.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVPTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRNIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGASKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(9225..10055)
                     /gene="amiD"
                     /locus_tag="ACHUQ7_003038"
     CDS             complement(9225..10055)
                     /gene="amiD"
                     /locus_tag="ACHUQ7_003038"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFR1136342.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(10052..10375)
                     /locus_tag="ACHUQ7_003039"
     CDS             complement(10052..10375)
                     /locus_tag="ACHUQ7_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFR1136343.1"
                     /translation="MQFSTTPTLEGLTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            complement(10586..>11019)
                     /locus_tag="ACHUQ7_003040"
     CDS             complement(10586..>11019)
                     /locus_tag="ACHUQ7_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR1136344.1"
                     /translation="CLFHECSMHGVKIKPWLPVKWTKELISDYLYGCLLSLYSICARD
                     IYNMNAGNNVKVAADAFLEIIFSLKNKYCIKLLSAQDRAFIYEFARMIFAYINDKSIE
                     ILLLSCFAAADQKAIQRYRPQSQDGEDFRSHLQYKLPLSAH"
BASE COUNT         2529 a         2916 c         2893 g         2681 t
ORIGIN      
        1 ccccctctga tgcataacat ttccttttaa ttgcaacttt actcgccctt ccgctttcag
       61 tagtgtgtca tcgcgcattt cgacttcttc tttgcaagga ctggattttc atgttttctg
      121 ggctgttaat cattctggtt cccctgattg tgggttacct cattccgctt cgccaacaag
      181 ctgcgttaaa agctattaat cagctattaa gctggatggt ttaccttatt ctctttttta
      241 tgggtatcag tctggcgttt ctcgataacc tcgccagtaa cctgttggcg attctgcatt
      301 attctgccgt cagtattacc gttattttac tgtgtaatat tgccgccctg atgtggctgg
      361 agcgaggcct gccgtggcgc aaccaccatc agcaagaaaa actcccgtcg cgtattgcga
      421 tggcgctgga gtcgctaaaa ctgtgcggcg tagtagtgat tggttttgcc attggtctga
      481 gtggactggc tttcttacaa cacgcgaccg aagccagtga atacacgtta attttgctac
      541 ttttcctcgt tggtattcag ttgcgcaata atggcatgac cttaaagcag attgtcctta
      601 atcgccgggg aatgattgtc gccgtggtgg tggttgtcag ttcattaatt ggtggtttaa
      661 ttaacgcctt tattcttgat ctccccatca ataccgcgct ggcaatggcc tccggtttcg
      721 gctggtattc tctttccggt attttattga ccgaatcttt tggtccggta atcgggagcg
      781 cggcgttttt taatgatctg gcccgtgaac tgattgctat tatgttgatc cctgggctga
      841 ttcgccgcag ccgctctact gcactgggct tatgcggtgc cacatcaatg gatttcaccc
      901 tgcccgttct tcaacgtact ggcgggctgg atatggtccc ggcggcaatt gttcacggtt
      961 ttattcttag cctgttagtg ccgatcctca tcgccttttt ctctgcgtaa tacctctctg
     1021 gcggtagttc cctgccgcca aaattgcgct aaatcaatct cccttaaagt tgcatgaaaa
     1081 atccctttta tccccgcgtt aagcgtctta cccttaaaca tgtatattaa atataacttt
     1141 aaaaggtgtg atcatgtttt gtgtgcaatg tgaacaaact atccgtactc cggcagaaaa
     1201 cggctgctca tacgcgcagg ggatgtgtgg taaaacggcg gaaacttctg accttcagga
     1261 tttactcatc gcggcgctgc aagggctttc tgcctgggcg gtaaaagcgc gtgaatacgg
     1321 catcatcaac cacgatgttg acagcttcgc gccacgtgcc tttttctcaa ccctgaccaa
     1381 cgttaacttc gattctccgc gtattgtcgg ctacgctcgt gaagcgattg ccttgcgcga
     1441 ggcgctgaaa gcacaatgcc tggctgtaga tgccaacgcg cgcgtcgata acccgatggc
     1501 tgacctgcaa ctggtgagcg atgatctcgg cgagctgcaa cgtcaggcag cagaatttac
     1561 ccctaacaaa gataaagcgg cgattggcga aaacattctc ggcctgcgtc tgctgtgcct
     1621 gtatggcctg aaaggtgcgg cggcctatat ggaacacgcg catgttctcg gtcaatacga
     1681 caacgatatt tatgcccagt accataaaat catggcgtgg ctggggacct ggcctgccga
     1741 tatgaacgcg cttcttgagt gttcaatgga aatcggccag atgaacttca aagtgatgag
     1801 cattctggat gcaggcgaaa ccggtaaata cggtcacccg acgccaaccc aggtcaacgt
     1861 caaagcgacg gcgggtaaat gcattctgat ttccggtcac gatctcaaag atctctacaa
     1921 cctgctggaa cagaccgaag gcacgggcgt taatgtctac acccacggcg aaatgctgcc
     1981 tgcgcatggc tacccggagc tgcgtaaatt caagcatctg gtcggtaact acggcagcgg
     2041 ctggcagaat cagcaagtgg agttcgctcg tttccctggc cccatcgtga tgacctcgaa
     2101 ctgcatcatc gacccaaccg taggcgctta tgacgatcgt atctggaccc gcagcatcgt
     2161 tggctggcct ggcgtgcgtc atctggatgg cgaagatttc tctgcggtca tcgcccaggc
     2221 gcaacaaatg gcgggcttcc cgtacagcga aattcctcac ctgatcaccg tgggctttgg
     2281 tcgccagacg ctgcttggtg cagccgatac gctgattgat ctggttagcc gtgaaaaact
     2341 gcgtcatatc ttcctgcttg gtggctgtga cggcgcacgc ggcgagcgcc actacttcac
     2401 cgatttcgcc accagcgtgc cggatgactg cctgatcctg accctcgcct gtggtaaata
     2461 tcgctttaac aaacttgagt ttggcgatat cgaaggtctg ccgcgtctgg tagatgcagg
     2521 tcaatgtaac gatgcttact cagcaattat tctggctgtc actctggcag agaaactggg
     2581 ctgtggtgtg aacgatctgc cgctgtcgct ggtgctctcc tggtttgaac agaaagcgat
     2641 tgtcattctg ctgacgctgc tttctctggg cgtgaaaaat atcgtcaccg gcccgactgc
     2701 acctggtttc ctgacaccag acctgctggc ggtgctgaac gagaaattcg gcctgcgttc
     2761 tatcaccact gttgaagaag acatgaagca actgttgaac gcgtaaggag tttgtcgatg
     2821 acgatgccaa cgaatcaatg cccgtggcgg atacaggttc atcacattac gcaagaaacg
     2881 ccggatgtgt ggacgatttc cctgatttgc cacgatcact acccatatcg cgccgggcaa
     2941 tatgcactgg tcagcgtgcg aaactcagcg gaaacgctgc gtgcttacac catttcctcc
     3001 acgccaggcg tgagtgaata tatcaccctg accgtgcggc ggattgatga cggtgtcggc
     3061 tcccagtggc tgacgcgcga tgtaaaacgc ggtgattatc tctggctttc ggacgcgatg
     3121 ggggaattta cctgcgacga taaagcagaa gataaattcc tgttgctggc ggcaggctgc
     3181 ggcgtcacgc cgattatgtc gatgcgtcgc tggcttgcga agaaccgtcc acaggccgat
     3241 gtgcgggtga tctacaacgt gcgtacgccg caggatgtta ttttcgccga tgagtggcgt
     3301 aactatccgg taacgctggt ggcagaaaat aacgttaccg aaggctttat cgctggtcgc
     3361 ctcactcgcg aactgctggc agatgtacct gacttagctt cacgtaccgt gatgacctgc
     3421 ggcccggctc cgtatatgga ttgggtagag caggaagtga aagcgctcgg cgtgacgcgt
     3481 ttctttaaag agaaattctt caccccagta gcagaagcgg cgaccagcgg tctgaaattc
     3541 accaaactgc aaccggcacg agaattttac gccccggttg gcaccacgct actggaggcg
     3601 ctggaaagca ataacgttcc ggttgtcgct gcctgccgtg cgggtgtttg cggctgctgt
     3661 aagacaaaag tggtttccgg tgaatatacg gtgagcagta caatgacgct gaccgacgcc
     3721 gaaatcgctg aaggttacgt actggcctgc tcctgccatc cgcaggggga tttggttctc
     3781 gcataatcgc cttatgcccg atgatattcc tttcatcggg ctatttaacc gttagtgcct
     3841 cctttctctc ccatcccttc cccctccgtc agatgaacta aacttgttac cgttatcaca
     3901 ttcaggagat ggagaaccat gaaacaaacg gttgcagctt atatcgccaa aacactcgaa
     3961 tcggcaggcg tgaaacgcat ctggggagtc acgggcgact ctctgaacgg tcttagcgac
     4021 agtcttaatc gcatgggcac catcgagtgg atgtccaccc gccatgaaga agtggcggcg
     4081 tttgccgctg gcgctgaagc acaactttca ggagaactgg cggtctgtgc cggatcgtgc
     4141 ggcccaggca acctgcactt gatcaacggc ctattcgatt gccaccgcaa taacgttccg
     4201 gtactggcga ttgccgctca tattccctcc agcgaaattg gcagcggcta tttccaggaa
     4261 acccacccac aagagctatt ccgcgaatgt agtcactatt gcgagctggt ttccagcccg
     4321 gagcagatcc cacaagtgct ggcaattgct atgcgcaaag cggtgcttaa ccgtggcgtt
     4381 tccgttgttg tattaccggg cgacgtggcg ttaaaacctg cgccagaagg ggcaaccacg
     4441 cactggtatc atgcgccaca gccagtcgtg atgccggaag aagaagagtt acgcaaactg
     4501 gcgcaactgc tgcgttattc cagcaatatc gccctgatgt gtggcagcgg ctgcgcgggg
     4561 gcgcataaag agttagttga gtttgccggg aaaattaaag cacctattgt tcatgcccta
     4621 cgcggtaaag agcatgtcga atacgataat ccgtatgatg ttggaatgac cgggttaatc
     4681 ggattctcgt caggtttcca taccatgatg aacgccgaca cgttagtgct actcggtact
     4741 cagttcccct accgtgcgtt ctacccgacc gatgccaaaa ttattcagat tgatattaat
     4801 ccagccagca tcggcgcgca tagcaaggta gatatggcgc tggtcggcga tatcaaatca
     4861 accctgcgcg cattgctgcc actggtggaa gaaaaaaccg atcgcaagtt tctggataaa
     4921 gcgctggaag attaccgcga tgcccgcaaa gggctggacg atttagctaa accgagcgag
     4981 aaagccattc acccgcaata tctggcgcag caaattagtc attttgccgc cgatgacgcc
     5041 atctttacct gtgacgtcgg cacgccaacg gtatgggcgg cacgttatct gaaaatgaac
     5101 ggcaagcgtc gcctgttagg ttcgtttaac cacggttcga tggctaacgc catgccgcag
     5161 gcgttgggtg cgcaggcaac cgagccggaa cgtcaggtgg tcgccatgtg cggcgatggc
     5221 ggttttagca tgttgatggg cgatttcctc tcagtagtgc agatgaaact gccggtgaaa
     5281 attatcgtct ttaataacag cgtgctgggc tttgtggcga tggagatgaa ggccggaggt
     5341 tacctgacag acggtactga gctgcacgac accaactttg cccgcattgc cgaagcgtgc
     5401 ggcattacgg gtatccgtgt agaaaaagcc tctgaagttg atgaagccct gcaacgcgcc
     5461 ttctctatcg acggtccggt gttggtggat gtggtggtcg ccaaagaaga attagccatt
     5521 ccaccgcaga tcaaactcga acaggccaaa ggttttagcc tgtatatgtt gcgcgcaatt
     5581 atcagcggac gcggcgatga agtgatcgaa ctggcgaaaa cgaactggct aaggtaaaaa
     5641 gggtggcatt tcccgtcata ataaggacat gccatgattg atttacgcag tgataccgtt
     5701 acccgaccga gccgcgccat gctcgaagcg atgatggccg ccccggttgg ggacgacgtt
     5761 tacggagacg accctaccgt taatgctctg caggactatg cagcagagct ttccggtaaa
     5821 gaagccgcca tttttctgcc taccggcact caggccaacc tggtcgctct gctcagtcac
     5881 tgcgaacgtg gcgaagagta tattgtcggt caggccgcgc ataactatct gtttgaagcc
     5941 ggtggcgcag cggtgctggg cagtattcag ccgcaaccca tcgacgcggc tgccgacggc
     6001 acgctaccgc tggataaagt ggcgatgaaa atcaaacccg acgatatcca tttcgcccgc
     6061 accaaattac tcagtctgga aaacacccac aacggcaaag tgctgccgcg tgaatacctg
     6121 aaagaagcat gggaatttac ccgcgagcgc aatctggcgc tgcatgttga cggtgcgcgc
     6181 atctttaatg ccgtggtggc ttacggctgc gaactgaaag agatcacgca atattgtgat
     6241 tcgttcacca tttgcctgtc gaaaggtctt gggacgccag tcggttcatt actcgtcggt
     6301 aatcgtgatt acattaaacg tgccatccgc tggcggaaaa tggccggtgg cgggatgcgt
     6361 cagtccggga ttctggctgc cgccgggatg tatgccctgg aaaataacgt cgcacggttg
     6421 caggaagatc acgacaacgc cgcctggatg gcggagcaac tgcgtgaagc aggcgcggat
     6481 gtgatgcgtc aggacactaa tatgctgttt gttcgcgtcg gggaagaaaa tgctgccgcg
     6541 ttaggcgaat acatgaaagc gagaaacgtg ctgattaacg cctcgccgat tgtccgcctg
     6601 gtgacgcatc ttgacgtctc gcgcgaacaa ctggcggaag tcgccgccca ctggcgcgca
     6661 ttcctggcgc gttaaggaga gagacgtgcc gcaacgcatt ttagttctcg gtgccagtgg
     6721 ctacattggt cagcatctgg tgcgcacact cagccagcaa gggcatcaga tcctagcggc
     6781 ggcacgtcat gtcgacaggc ttgcaaagct gcaactggca aacgtcagtt gccataaagt
     6841 cgatctcagc tggccggata accttccggc cctgttgcag gatatcgata ccgtctattt
     6901 tctggtgcac agcatgggcg aaggcggcga ttttatcgct caggagcgcc agctggctct
     6961 caacgtccgc gatgcgctac gtgaagtacc agttaagcaa ttaatctttc tcagttcgtt
     7021 gcaggccccg ccacatgagc agtcggatca tctgcgagct cgtcaggcta cggcggacat
     7081 tcttcgtgaa gcgaatgtac cagtgaccga acttcgggcc ggaattatcg ttggcgcagg
     7141 ttcagcggcg ttcgaagtca tgcgcgatat ggtctacaac ctgccagtgt taacgccgcc
     7201 acgctgggta cgttcacgca ccacgcccat cgcgctggaa aacttgctgc actatctggt
     7261 ggcgctgtta gaccatccag ccaacgaaca ccgcatcttc gaagccgccg gaccagaggt
     7321 gctcagttat cagcaacagt ttgaacattt tatggcggtg agcggtaagc gccgctggtt
     7381 gatccccatc cccctcccca cccgctggat ttcggtgtgg tttctcaatg tgattacttc
     7441 cgtaccgccc accaccgcca gggcgttgat tcaggggctg aaacacgatc tgctggcaga
     7501 tgataccgcg ctacgtgcac tcatcccaca acggctgatc gctttcgatg acgcggtacg
     7561 tagcacgttg aaagaggaag aaaaactggt caactccagc gactggggct acgacgctca
     7621 ggcctttgcc cgctggcgac cggagtacgg ttatttcgcc aaacaggcgg ggtttaccgt
     7681 taaaacgtcc gccagccttg cggctttatg gctggtggtg aaccaaatcg gcggtaaaga
     7741 gcgttatttc tttggcaata ttttgtggca gacacgggcg ttgatggacc gcgcgatcgg
     7801 tcataatctg gcgaaaggcc gcccggagcg cgaatattta cagaccggcg atgcggtgga
     7861 tagctggaaa gtgattgtcg ttgaaccgaa aaagcaactt acgttgttat ttggcatgaa
     7921 agcgccgggg ctgggacgac tgtgttttac cctggaagat aaaggtgact atcgtactat
     7981 cgatgtccgc gctttctggc atccgcacgg tatgccgggg ctgttttact ggttattgat
     8041 gatccccgcg catctgttta tttttcgcgg aatggcaaaa caaatcgcca gactggcaga
     8101 acaaagcaca gattaatcga caaaacctgc gttattcttt catgtttccc attgcatcat
     8161 gggggaaatc acggaagaat gcgcacgtca ttttctcaac aacagtggat tgatatgaag
     8221 gtactggtta ccggcgccac cagcggctta ggtcgaaacg cggtagagtt tttatgccag
     8281 aaaggcatca gcgtgcgtgc gagcggtcgc aacgaagcaa tgggtaaatt gctggaaaaa
     8341 atgggcgcag agtttgttcc gacggatctg accgagctgg tttcatcaca agctaaagtg
     8401 atgctcgcgg gcattgatac gctgtggcac tgctccagct ttacctcacc ctgggggaca
     8461 caacaggctt tcgatctggc taacgttcgc gccactcgcc gcctgggtga atgggctgtc
     8521 gcctggggtg tgcgtaactt tattcatatc tcttccccct ccctgtactt cgattatcac
     8581 caccatcgca atattaaaga agattttcgc cctcaccgct tcgccaacga gtttgcccgc
     8641 agcaaagcag ccagcgaaga agtgatcaat atgctttcgc aggcgaatcc acaaacgcgc
     8701 tttactattc tgcgcccaca aagtctgttc ggaccgcacg ataaagtctt tattccccgt
     8761 ctggcgcata tgatgcacca ctacggcagt attctgttac cgcatggcgg cagtgcgctg
     8821 gtcgatatga cctactatga aaatgccgtg cacgcaatgt ggctggcaag ccaggaagcc
     8881 tgcgataagc taccttccgg gcgtgtgtac aacatcacca acggcgagca tcgcacactg
     8941 cgcagcatcg tgcagaagct gatcgacgag ttgaatattg actgtcgtat tcgttccgtc
     9001 ccttacccga tgctggatat gatcgcccgc agcatggagc gtttaggccg caagtcagca
     9061 aaagagccgc cgctgaccca ctacggcgcc tccaagttga attttgactt tacgctggat
     9121 attacgcgtg cgcaggaaga gttaggttat cagccggtca tcaccctgga tgaaggcatc
     9181 gagaaaactg ccgcctggct acgcgaccac ggaaaactgc cgcgctaatc ctgcccgtat
     9241 ttctccagca atgcttcggc aatcgcctga gtttctgcat ccgcttcgcc gttatataac
     9301 gtcgggcgga aatgcatctg gaatgccata atcacgcgcc gctgctcgcg cggtgtcata
     9361 tcaggtttta cgtcataacc gtagcgcgcc aaaagctcca gcaatgacgc ggtatctacc
     9421 ggtgtgtgcg gcgcgcgccc ggcaaggtaa aagttcaccc gctgcgcatc cggccaggca
     9481 ccaatcccct gctgcgccag ttgctgccag ggaaataatg gccccggatc gtctttgcgc
     9541 tgcggtgcga tatccgcatg tgccactacg ttttccggct tgatgtgata acgggcaata
     9601 atatctttcg ccagcggaat aagcgcctga atctgtgcct gttcaaacgg ggcaaaatat
     9661 ttcactccgg atgatttttg ccagccacgg ttttccagct caatgccaat agaggtgtcg
     9721 ttaaggcgcg ttgccccgcg ccaggcgcta atccccgcat gccaggccag ttcttgttcc
     9781 ggcaccagtt gccagatgcg cggtttaccg ttgtatcgcg gtggcaccgc agggatcaga
     9841 taatgcgagc tgacctgctt atcagtcagt gtcgccagcg agctatcaaa atcatctgcg
     9901 gtgtagtgga tcaccagcac tttaatgcgc ggatacgccg cctgcgcctg gtgtcgggta
     9961 tcaagctgat atccctcttt ctcgacaatg cctttttcgc ctgcacaccc tgccagtaac
    10021 agagcggcag cgaccagcca gaagactctt ctcatcgacg cgttttcacc gccgtaccgc
    10081 tgacgctaac catcagcata ctgccgtttt gcccgaccgt ttcgtagtcg atatcaatac
    10141 caactacggc atcggccccc agcgcccgcg cctgggagcc taattcctca aaggcgatct
    10201 cccgtgcttt acgcagttct ttttcatacg caccggaacg tccgccaacg atatcgcgga
    10261 taccggcaaa gaaatcacgg aaaatattgg cacctaaaat cgcttcgccg gtcaccacac
    10321 cgcaatattc aacgatggtc aggccttcca gagttggggt tgtagaaaat tgcatgatcg
    10381 atctccttgt ttgttaagcc ttcgatctct aaagcattat cggactgata tgccatgttg
    10441 ggaagaaggt tttgatcagt ttaggaaatc atctcattgt gtcaaaaaca cccacttaag
    10501 tctttacgat tatccgttgc ataacccgat attggtttca ttatagggta ccgcttacct
    10561 gtcttattat gttgcaccaa caacattaat gagcggaaag aggcaactta tattgtaaat
    10621 gactcctaaa gtcttcccca tcttgtgact gaggtctata ccgctggatt gctttttggt
    10681 cagccgccgc aaaacaagaa agtaataaga tctcaatgct tttgtcatta atataagcga
    10741 agatcattct tgcaaactcg tatataaacg cccgatcctg agcgcttaat agtttaatac
    10801 aatatttatt tttaagagag aaaataattt caaggaaagc atccgcagca accttcacgt
    10861 tattcccggc gttcatatta tatatatccc ttgcacagat agagtacaga gatagtaaac
    10921 agccatacaa ataatcactt attaattctt ttgtccattt tacaggcaac cagggtttta
    10981 tcttgacacc atgcatacta cattcatgaa aaagacatt
//
LOCUS       DAZPCS010000123        10991 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZPCS010000123 DAZPCS010000000
VERSION     DAZPCS010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10991
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.123"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            362..1702
                     /gene="dcuB"
                     /locus_tag="ACHUQ7_003041"
     CDS             362..1702
                     /gene="dcuB"
                     /locus_tag="ACHUQ7_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFR1136345.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            1780..3426
                     /gene="fumB"
                     /locus_tag="ACHUQ7_003042"
     CDS             1780..3426
                     /gene="fumB"
                     /locus_tag="ACHUQ7_003042"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFR1136346.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAENNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            3549..4178
                     /locus_tag="ACHUQ7_003043"
     CDS             3549..4178
                     /locus_tag="ACHUQ7_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFR1136347.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(4317..5738)
                     /gene="melB"
                     /locus_tag="ACHUQ7_003044"
     CDS             complement(4317..5738)
                     /gene="melB"
                     /locus_tag="ACHUQ7_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HFR1136348.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQSSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(5841..7196)
                     /gene="melA"
                     /locus_tag="ACHUQ7_003045"
     CDS             complement(5841..7196)
                     /gene="melA"
                     /locus_tag="ACHUQ7_003045"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFR1136349.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICENMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPYIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKLGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPSWLH
                     R"
     gene            7479..8387
                     /gene="melR"
                     /locus_tag="ACHUQ7_003046"
     CDS             7479..8387
                     /gene="melR"
                     /locus_tag="ACHUQ7_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFR1136350.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSSWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            8586..10853
                     /gene="adiA"
                     /locus_tag="ACHUQ7_003047"
     CDS             8586..10853
                     /gene="adiA"
                     /locus_tag="ACHUQ7_003047"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFR1136351.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKHHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPRFEHETEG
                     TEIIDGIYHVMCVKA"
BASE COUNT         2836 a         2891 c         2679 g         2585 t
ORIGIN      
        1 gtgtattttt acccacaaat gggtagatca gattaatcta taaacctaat gatatctgcc
       61 ctgagaacaa aaaatagacc gataaatatc aataagataa cagcaaacaa aacattaaca
      121 tctgcgcagt acaaactata aacccatcgc cagagagttt ttctctctga aaaagccgct
      181 tatcacagtg cataaatttg ccgctgcttt aatcagccaa tattcactgt gaggtatttg
      241 ctaaagccgg taacgaccaa acggatattt agtcaggctc tgaaaacagt tcatacaaaa
      301 cagaacgtga ctgtgatcta ttcagcaaaa atttaaatag gattatcgcg agggttcaca
      361 catgttattt actatccaac ttatcataat actaatatgt ctgttttatg gtgccagaaa
      421 gggtggtatc gcgctgggtt tattaggcgg tatcggtctg gtcattctgg tcttcgtctt
      481 ccaccttcag ccaggtaaac caccagttga tgtcatgctg gttatcattg cggtggtggc
      541 ggcatcggcg accttgcaag cttcgggcgg tcttgatgtc atgctgcaaa ttgccgagaa
      601 gctgctgcgc cgcaacccga aatatgtctc aattgtcgcg ccgtttgtga cctgtacact
      661 gaccattctt tgcggtacgg gtcatgtggt ttacaccatt ctgccgatca tctacgacgt
      721 cgccattaag aacaacatcc gtccggaacg tccgatggcg gcaagttcta tcggtgcaca
      781 gatggggatt atcgccagtc cggtgtcggt tgcggtcgtg tctctggttg cgatgctggg
      841 taatgtcacc tttgatggtc gccatcttga gttcctcgat ctgctggcaa tcaccattcc
      901 atcgacgtta atcggtatcc tggcgatcgg tatcttcagc tggttccgcg gtaaagatct
      961 ggataaagac gaagagttcc agaaattcat ctccgtaccg gaaaaccgtg agtatgttta
     1021 cggtgatacc gcgacgctgc tggataaaaa actgccgaaa agcaactggc tggcaatgtg
     1081 gattttcctc ggggcaatcg ctgtagtcgc ccttcttggt gctgattcgg acctgcgtcc
     1141 atccttcggc ggcaaaccgc tgtcgatggt actggttatt cagatgttta tgctgctgac
     1201 cggggcgctg attattatcc tgaccaaaac caatcccgcg tctatctcaa aaaacgaagt
     1261 cttccgttcc ggtatgatcg ccatcgtggc ggtgtacggt atcgcatgga tggcagaaac
     1321 catgttcggt gcgcatatgt ctgaaattca gggcgtactg ggtgaaatgg tgaaagagta
     1381 tccgtgggcg tatgccattg ttctgctgct ggtttccaag tttgtaaact ctcaggctgc
     1441 ggcgctggcg gcgattgttc cggtcgcgct ggcgatcggc gttgatccgg catacatcgt
     1501 ggcttcagca ccggcttgct acggttatta catcctgccg acttatccga gcgatctggc
     1561 agcgattcag tttgaccgtt ccggcaccac ccacatcggt cgcttcgtca tcaaccacag
     1621 ttttattctg ccggggttga ttggtgtgag cgtatcgtgc gtcttcggct ggatcttcgc
     1681 cgcgatgtac gggttcttat aaatgcactt tgcgtgccgc ccgtgactac gcggcacgcc
     1741 attttcgaat aacaaataca gagttacagg ctggaagcta tgtcaaacaa accctttatc
     1801 taccaggcac ctttcccgat ggggaaagac aataccgaat actatctact cacttccgat
     1861 tacgttagcg ttgccgactt cgacggcgaa accatcctga aagtggaacc agaagccctg
     1921 accctgctgg cgcagcaagc ctttcacgac gcttctttca tgctacgtcc ggcacaccag
     1981 aaacaggttg cggctattct tcacgatcca gaagccagcg aaaacgacaa gtacgtggca
     2041 ctgcaattct taagaaactc cgaaatcgcc gccaaaggcg tgctgccgac ctgccaggat
     2101 accggcaccg cgatcatcgt cggtaaaaaa ggccagcgcg tgtggaccgg tggcggtgat
     2161 gaagaagcgc tgtcgaaagg cgtctataac acctatatcg aagataacct gcgctactcg
     2221 caaaacgcgg cgctggacat gtacaaagag gtcaacaccg gcactaacct gcctgcgcaa
     2281 atcgacctgt acgcggtaga tggcgatgag tacaaattcc tttgcgttgc gaaaggcggc
     2341 ggctctgcca acaaaacgta tctctaccag gaaaccaaag ccctgctgac tcccggcaaa
     2401 ctgaaaaact tcctcgtcga gaaaatgcgt accctcggta ctgcagcctg cccgccgtac
     2461 catatcgcgt ttgtgattgg cggtacgtct gcggaaaaca acctgaaaac cgtcaaatta
     2521 gcaagcgctc actattacga tgaactgccg acggaaggga acgaacatgg tcaggcgttc
     2581 cgcgatgtcc agctggaaca ggaactgctg gaagaggccc agaaactcgg tcttggcgcg
     2641 cagtttggcg gtaaatactt cgcgcacgac attcgcgtta tccgtctgcc acgtcacggc
     2701 gcatcctgcc cggtaggcat gggcgtctcc tgctccgctg accgtaacat taaagcgaaa
     2761 atcaaccgcg aaggtatctg gatcgaaaaa ctggaacaca atctaggtca gtacattcca
     2821 caagaactgc gtcaggccgg tgaaggcgaa gcggtgaagg tcgaccttaa ccgcccgatg
     2881 aaagagatcc tcgcccagct ttcgcaatat ccggtttcca ctcgtttgtc gctcactggc
     2941 accattattg tgggtcgaga tattgcacac gccaagctga aagagctgat tgacgccggt
     3001 aaagaacttc cgcagtacat caaagatcac ccgatctact acgcgggtcc ggcgaaaacc
     3061 cctgccggtt atccatcagg ttcacttggc ccaaccaccg caggtcgtat ggactcctat
     3121 gtggatctgc tgcaatccca cggcggcagc atgatcatgc tggcgaaagg caaccgcagc
     3181 cagcaggtta ccgacgcgtg tcataaacac ggcggcttct acctcggtag catcggcggt
     3241 ccggcggcgg tactggcgca gcagagcatc aagcatctgg aatgcgtcgc ttatccggag
     3301 ctgggtatgg aagctatctg gaaaatcgaa gtagaagatt tcccggcgtt tatcctggtc
     3361 gatgacaaag gtaacgactt cttccagcaa atcgtcaaca aacagtgcgc gaactgcact
     3421 aagtaacctc ttcggcccag cgcctggcag catgctgcca ggtgatcccc ctgggccacc
     3481 tcttttgcga ttgtaatttc atgcttgctg gtgaatagtc agtattttcc ctgacttgcg
     3541 aactcaccat gacccgcaca ctcaagccgt taattcttaa cactggcgca ctggcgctaa
     3601 cgttaatcct gatttatacc ggcatttcgg cccatgacaa actcacctgg ctgctggaag
     3661 tgacaccggt gattatcgtc gtgccgctac tacttgccac cgccagacgt tacccgttaa
     3721 cgctgctgct ctatacgctc atcttctttc acgccatcat cctgatggta ggcgggcagt
     3781 acacctacgc gaaagtcccc gttggttttg aggtgcagga atggctgggg ttgagtcgta
     3841 atccgtatga caagctgggg cactttttcc aggggctggt gcctgcactg gtggcacgag
     3901 aaattctcgt tcgcgggatg tacgtgcgcg gacgtaaaat ggtggcgttt ctggtctgtt
     3961 gcgtggcgct ggcgataagc gcgatgtatg aattaatcga gtggtgggcg gcactggcga
     4021 tgggccaggg agcggatgat tttctcggta cccagggcga ccagtgggac acgcaatccg
     4081 atatgttctg cgcgctgctt ggcgcattaa cgacagtgat attgctcgct cgctttcatt
     4141 gccgccagct acggcgctat ggcttgataa ctggataaca acgtgcactt tgttgatgcc
     4201 ggatgcggcg tgaattcctt atccggtcta caaaatttgc aaatccaaag cattacatta
     4261 agacgtggcc tgataaacat atcgcatcag gccattttcg ttagctattg tccacgtcag
     4321 gctttcacat cactcactgc accgaccgga atatcagcat gaacaggctc gggcggtact
     4381 ttgcgatatt tatccagcag atgaatctgg atcctgcgca gcgtgtcgcc attgaggcga
     4441 tagaaacgga agtagagaat cagcgttacc atgaaaaaca gagtcggtag agcaatcata
     4501 ataaactgca tacctaatag ggcttgcgta gactgttcaa cattcggtac atagccaatc
     4561 atccctaaca caaccgcaat gaaaaaagcc gcaaaggctg aaccgccctt caccaccata
     4621 gtctgcacgg agtaagcgat gctttcacag cgtacgtgca gtttatattc accgtaatca
     4681 acggtatctg ccaccatgat gacctgtaat acccagaaaa gcgccgttcc cacattcagc
     4741 aaaatacccg caatcacaat gaggacgacg ttgtgatagc tcattaatgc cattaacagg
     4801 agaacaccac agcttaacac cggaagaata gatgctccgg cccataaaat gcgtcgggat
     4861 aatgatttaa ctaagcgtgg gaagaatact aacgtcacca ggttagcagc tcccgcatac
     4921 gacagataat aggggaacaa atccgcatca ccgataacat atgagaaata atagatagca
     4981 aatccggtaa taatgttgct ggctacatta taagcaagag ccatacccaa gaggcatgaa
     5041 agctgatcgt ttttataaat aagcgcaacg atggctttaa gtgtcagatg gcttccttca
     5101 gcagacgatt gattgtctga cgaaaagact tcatgcacat tgcgcagagt gatgatggtt
     5161 gaaacaataa aaaaggcgat caataccaga gtgaacatct gaaagccaaa tccccgatcg
     5221 ccaccgccga cataattaac aaatggtagc gtcacacctg ccgtaacaaa gcctgccaga
     5281 ctggcaaaaa aacgcggata aggaaccagt tgttcgcgtt cacgtttatc gagcgtgatg
     5341 gttggaacca gcgaccagaa gggaatatcc ataatggtgt aagtcatgcc ccagaggatg
     5401 taggtcacgc aaacaaagac aatctgagta gtaccttcaa acagatgcgc actaaagagg
     5461 agaaataaga ttacagagtt tgccaacgta ccgatcagga tccagggttt gaacttaccc
     5521 catcgcgatc gcgtagcatt tacaatccat cccataatcg gatcgttaat agcatcccag
     5581 atcctcgcca ccagaaacaa agtaccgacc aaacccacag acagcccgac gacatcggtg
     5641 tagtaataca tgaggtacat atacacaatg ccgatcgcaa aatccttccc gaacgctcca
     5701 aatccataac tgagttttgt agtcattgaa atgctcatag ggtatcgggt cgctgttacc
     5761 aacgcccgcc ttctgttatt ggtatcgtca gaggtgtgcg ccggaataaa tcccccggcg
     5821 cagtagcgtt tagtcgcgtt ttaacggtgc aaccagcttg gcagccagtc gccgtgggcg
     5881 gcaatcaggt cgtcaacaag agcatatatt tcgtcaatgc ccagcacggc ggcagtatgc
     5941 gggtccatca tcgcggcgtg gtaaacacgg tcgcgatttt ccgtaagaat agcttcggtc
     6001 agcagcgtct gtacgttgat gttggtttgc atcagggcgg ccagatgcga aggtagcgta
     6061 ccgactttgg tcggctgaat gccattagca tcaaccagac aggctacttc cacgcaacat
     6121 ccttgtggca ggttatcaat caaaccatcg ttacggacgt tgccgtaaat cacactcggc
     6181 tcgccagtcc agatagcgtt catgattgtg ctggcatatt cccgtgacgg tttaatatca
     6241 atccgggagg cgtttttata ctcctccagc tctttatgcc agttcgccag ctgctcgacg
     6301 cagcgtttcg ggtactcatc cagcggtact ttataacgct caatcaaatc ctcacgacct
     6361 agcttaataa accacggcgt atattcggca aaatgttctg atgattccgt gacgaagtag
     6421 cccagctttt tgaacatttc gtagcgcaca atattctggc agcgagtatt accatgaata
     6481 tttggcttcg gtgcctgccc tgcttcataa gccgccagca gttccgggta gagattcacg
     6541 taactgccgt cggcggtttt gcgctccagc tccaggtaaa acgccatatg gttgatacct
     6601 gcgcaacggt aacgcagcgt agccgggtcg atattgaggt cacgcgccag ctcttccgcc
     6661 gttccctgca ccgaatggca cagcccgacc tgtttgatat acggatagcg ggcatacatc
     6721 gcccaggtat tcatcgccat tgggttaaca tagttgagca tggtggcatc ggggcagact
     6781 tccgtcatgt tctcgcaaat ttgccacaga tgcggaatgg tacgtagcgc gcgcataata
     6841 ccgcccggcc ccaacgtatc ggcaatggtt tgttccagac catgccgctt acagacctcg
     6901 aaatcagtca ccgtgcaagg ttcataaccg ccaatctgaa atgccaccac gacaaaatcg
     6961 gcatcctcta aggcttcttt ctgttgggtg tggcaggtga ttttgccgct ggcccctgct
     7021 gaatccatca gcttacgcac cacaatatgc gactcttcca ggcgggtggg gtcaatgtcc
     7081 atcagggcaa tatgcgccgt tttcagcgcc tcgcgatgga acacatcacc aagaatattt
     7141 ttaacgaaaa tcgtcgaacc agcgccgata aatgtaattt tgggtgcaga catcatgcag
     7201 atctcctggc ttgcttgaat aacttcatca tggcaggcga atctgcagaa aacctccggc
     7261 ttccaggttg agcattccca taaactcaga tcctgcgtga agcagcagta aatctgagtt
     7321 tatgggaata aattacctga aaagtagagt tttcggcctc atgatgctcg gacagtcccc
     7381 tcgcccctct gggaagaggg tgaaaaccgt gctcccactc gcagtcatcc tccctcactc
     7441 ctgccataat tctgatattc taggaaagag agccatccat gaatacagat acgtttatgt
     7501 gcagcagcga cgaaaaacag acccgcagcc cgctgtcgct gtattcggaa taccagcgga
     7561 tggagattga gtttcgcgca ccgcatatca tgcccaccag ccactggcat ggtcaggtcg
     7621 aagtgaatgt gcctttcgat ggcgatgtgg aatacctgat caacaatgaa aaagtgaata
     7681 tcaatcaggg tcatatcacg ctgttctggg cctgtacacc gcaccaacta acagataccg
     7741 gaacctgtca gagcatggcg atttttaatc tgccgatgca tctgtttctc tcctggccgc
     7801 tggataaaga cctgattaac cacgtcactc acggcatggt gatcaaatca cttgccacac
     7861 agcaacttag cccgtttgaa gtacgccgct ggcagcagga attaaacagt ccgaatgagc
     7921 aaattcgcca gctcgccatt gatgaaattg gcctgatgct caagcgattt agcctctcta
     7981 gctgggaacc gattctggtc aataaaacct cgcgcacaca caaaaacagc gtctcgcgcc
     8041 atgcgcaatt ttatgttagc cagatgctgg gctttattgc cgaaaactat gatcaggcgc
     8101 tgaccatcaa cgatgtggct gagcacgtca aacttaacgc caactatgca atggggatat
     8161 tccagcgggt catgcaattg acgatgaaac agtacattac cgcgatgcgc atcaaccacg
     8221 ttcgcgcgtt actgagcgat accgataaaa gtattctcga tattgccctg acggcaggct
     8281 ttcgttcaag tagtcgtttt tacagcacgt tcggcaaata tgtcggcatg tcgccgcaac
     8341 aataccgcaa acttagccaa cagcgccgcc agacgtttcc cggctaaaga cgatatcagt
     8401 atcagccaaa aaaatagttt agccgtcgat aataagcagt gaaaataatt taaaataatc
     8461 acataacgta ttgtttataa aactaaatat ttcaaaaatg tttgtttttc acgcgcttta
     8521 cagcccgaaa aggccggaag atacttgccc gcaacgaaga ttccttcata accgggtaag
     8581 caatgatgaa agtattaatt gttgaaagcg agtttctcca tcaagacacc tgggtcggta
     8641 acgccgttga gcgtctggca gatgctttaa gccagcaaaa tgttaccgtg attaaatcca
     8701 cctcctttga tgatggtttt gccattctct cttcaaacga agccattgac tgcctgatgt
     8761 tcagctatca aatggagcat ccggacgaac atcaaaacgt cagacaattg atcggtaagc
     8821 ttcatgagcg ccaacaaaac gtgccggtct tcctgttggg cgatcgggaa aaagccctcg
     8881 ccgcaatgga ccgggatctg ctggagcttg tcgatgaatt cgcctggatt ctggaagata
     8941 ccgccgactt tatcgccgga cgcgccgttg ccgcgatgac ccgctaccgc cagcagctgt
     9001 tgccgccact gttcagcgcg ctgatgaaat atagtgacat ccatgaatat tcctgggcag
     9061 cgccaggcca ccagggcggc gttggtttta ccaaaacacc cgccggacgt ttctaccatg
     9121 actactatgg tgaaaatctg ttccgtaccg acatgggcat cgaacgaact tccctcggtt
     9181 ctttgcttga ccatactggc gcatttggcg aaagcgaaaa atatgccgca cgcgtatttg
     9241 gtgccgatcg ctcctggtcg gtagtcgtcg gtacttccgg ctctaaccgc accatcatgc
     9301 aggcttgcat gaccgataac gatgtcgtgg tcgttgaccg taactgccat aaatccatcg
     9361 aacaaggttt gatgctgaca ggcgcgaaac cggtctatat ggtgccaagc cgcaaccgct
     9421 acggcattat cgggccaatc tatccgcagg aaatgcaacc tgaaaccttg caaaagaaaa
     9481 tcagtgaaag cccgctgacc aaagacaaag ccgggcaaaa accgtcttac tgcgtggtaa
     9541 ccaactgcac ctatgacggc gtgtgttata acgctaaaga agcgcaggat ctgctggaaa
     9601 aaacctccga tcgtctgcac tttgacgaag cctggtacgg ctatgcacgt ttcaacccga
     9661 tctatgccga tcactatgcc atgcgcggcg aacctggcga tcacaacggt cctaccgttt
     9721 tcgccaccca ctccatccac aaactgctga atgcgctgtc acaggcttct tatattcatg
     9781 tacgtgaagg tcgtggggcg attaacttct cccgcttcaa ccaggcctac atgatgcatg
     9841 ccaccacctc cccgctgtat gccatctgcg catccaacga cgtggcggtg tcgatgatgg
     9901 acggcaacag cggcctgtca ctgacacagg aagtgattga cgaagcggtt gatttccgtc
     9961 aggcgatggc gcggctatat aaagagttca ccgctgacgg tagctggttc ttcaaaccgt
    10021 ggaacaaaga agtcgtcacc gacccacaaa ccggcaaaac ctatgacttt gctgacgcac
    10081 caaccaaact gctgaccacc gttcaggact gctgggtaat gcatccgggc gaaagctggc
    10141 acggcttcaa agatattccg gataactgga gtatgctcga cccaattaaa gtcagcatcc
    10201 ttgctccggg aatgggtgaa gatggtgaac tggaagaaac cggtgttccg gcggcgctgg
    10261 tcactgcctg gcttggtcgc cacggcattg tgcctacccg caccactgac ttccaaatta
    10321 tgttcctgtt ctctatgggc gtaacccgtg ggaaatgggg gactctggtt aacacccttt
    10381 gctccttcaa acaccactat gacgccaaca caccgctggc gcaggtgatg ccggaacttg
    10441 ttgaacaata tcctgacact tacgcgaaca tggggattca cgatctgggt gacaccatgt
    10501 ttgcctggct gaaagaaaac aaccctggcg cacggttgaa cgaagcctat tccggcctgc
    10561 cggtggcgga aatcaccccg cgtgaagcgt acaacgcgat tgtcgacaac aatgtcgaac
    10621 tggtatccat tgaaaatctg ccaggacgca tcgcggcaaa ctcagttatc ccgtatccgc
    10681 caggaatccc gatgctgctg tctggtgaaa acttcggcga taaaaacagt ccgcaagtaa
    10741 gttatttacg ctcgctgcaa tcctgggacc accatttccc tagatttgaa cacgaaactg
    10801 aagggactga aattattgac ggtatttacc acgttatgtg cgtgaaagcg taaccactat
    10861 tccgctgaag gcgtaattgt ttaaataaca ttacgccgcc tggccttagg ccttttgagt
    10921 atggcaacgt tttcataaaa attgctgcaa acaaaaatgt catacttttt gcgcggcccc
    10981 accccacgct t
//
LOCUS       DAZPCS010000124        10973 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZPCS010000124 DAZPCS010000000
VERSION     DAZPCS010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10973
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.124"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            73..834
                     /gene="adiY"
                     /locus_tag="ACHUQ7_003048"
     CDS             73..834
                     /gene="adiY"
                     /locus_tag="ACHUQ7_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFR1136352.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            971..2308
                     /gene="adiC"
                     /locus_tag="ACHUQ7_003049"
     CDS             971..2308
                     /gene="adiC"
                     /locus_tag="ACHUQ7_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFR1136353.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            2412..4055
                     /gene="eptA"
                     /locus_tag="ACHUQ7_003050"
     CDS             2412..4055
                     /gene="eptA"
                     /locus_tag="ACHUQ7_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFR1136354.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILIGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAELIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            4052..4720
                     /gene="basR"
                     /locus_tag="ACHUQ7_003051"
     CDS             4052..4720
                     /gene="basR"
                     /locus_tag="ACHUQ7_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFR1136355.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4721..5821
                     /gene="pmrB"
                     /locus_tag="ACHUQ7_003052"
     CDS             4721..5821
                     /gene="pmrB"
                     /locus_tag="ACHUQ7_003052"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFR1136356.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(5797..5886)
                     /gene="pmrR"
                     /locus_tag="ACHUQ7_003053"
     CDS             complement(5797..5886)
                     /gene="pmrR"
                     /locus_tag="ACHUQ7_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFR1136357.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(5998..7500)
                     /gene="proP"
                     /locus_tag="ACHUQ7_003054"
     CDS             complement(5998..7500)
                     /gene="proP"
                     /locus_tag="ACHUQ7_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFR1136358.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7764..8642)
                     /locus_tag="ACHUQ7_003055"
     CDS             complement(7764..8642)
                     /locus_tag="ACHUQ7_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HFR1136359.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            complement(8639..10867)
                     /gene="crfC"
                     /locus_tag="ACHUQ7_003056"
     CDS             complement(8639..10867)
                     /gene="crfC"
                     /locus_tag="ACHUQ7_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFR1136360.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELCKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAEMESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
BASE COUNT         2745 a         2865 c         2690 g         2673 t
ORIGIN      
        1 tatataacca tttcccggta tcgctttgct ttagcgagaa ctggtctttt attcgcactc
       61 aggagtacat gtatgaggat ttgcagcgac caaccttgta ttgttttatt gactgaaaaa
      121 gatgtctgga taagggtgaa tgggaaagaa cctattagcc ttaaagctaa ccatatggcg
      181 ttattaaatt gtgaaaataa tattatcgac gtctcctctc ttaacaacac tttggttgct
      241 catattagtc acgacatcat caaagattac ctccggtttc tgaataaaga tctctcgcaa
      301 ataccagtat ggcaacgtag cgctacgccc atactcaccc tgccatgcct gacgccagac
      361 gtctttcgcg ttgccgcgca acacagcatg atgcccgcag aaactgagtc agaaaaggaa
      421 cgaacacgcg cattattatt cacggtgcta tcccgttttc tcgacagtaa aaaattcctt
      481 tcattaatga tgtatatgtt acgtaattgt gtaagtgaca gcgtttatca aattattgaa
      541 agcgatattc acaaagactg gaatcttagt atggtagcca gttgtttatg tcttagccca
      601 agtctgttaa agaaaaagtt gaaaagcgaa aacaccagtt atagccaaat aatcaccacc
      661 tgccgcatgc gttatgccgt aaatgaatta atgatggacg gtaaaaatat ctctcaggta
      721 tcacagtcct gcggctacaa cagtacgtcg tactttattt ctgtctttaa agacttctac
      781 ggtatgacgc cgctgcatta tgttagtcag cacagagaac gcactgtcgc ctgattttta
      841 accttaacga agagctatat taataacggc atcagcgata acccggtcga taataattca
      901 actatcgaat gcaggcgtat gatatgacgt aatttattgt cacgaagctc gccttcgcag
      961 gagtttaatt atgtcttcgg atgctgatgc tcacaaagtg ggcttaatcc ccgtcaccct
     1021 gatggtgtcg gggaatatta tggggtcagg tgtttttctg ttacctgcaa acctggcctc
     1081 cactggcggg attgccattt atggatggtt ggtgacgatt atcggtgcgc tggggctctc
     1141 gatggtatac gccaaaatgt cgttcctcga cccaagtcct ggtggttctt acgcttacgc
     1201 ccgccgctgc tttggcccgt ttctcggtta tcaaaccaac gtcctctact ggctggcctg
     1261 ctggatcggc aatatcgcca tggtggtcat tggcgtagga tatttaagtt acttcttccc
     1321 gattctgaaa gatccattgg tattaaccat cacctgcgtc gtggtgctgt ggatcttcgt
     1381 cctgctgaac attgtcggtc cgaaaatgat cacccgcgtg caggccgttg ccactgtgct
     1441 ggcgctgatc cccatcgtcg ggattgccgt atttggctgg ttctggttcc gtggtgaaac
     1501 ctatatggcg gcatggaacg tcagcggcct gggcaccttc ggtgcgattc aaagtaccct
     1561 taacgttacg ctgtggtcgt tcatcggtgt ggaaagtgcc tccgttgccg caggtgtggt
     1621 gaaaaacccg aaacgcaatg tccctatcgc caccattggt ggggtattga ttgccgccgt
     1681 ttgctatgtg ctctccacca ccgcgattat ggggatgatc cctaatgccg cactgcgcgt
     1741 ttctgcctcg ccattcggtg atgccgcacg gatggcgttg ggtgacaccg ctggggccat
     1801 tgtttccttc tgcgcagctg cgggttgctt aggttcactg ggcggctgga cgttgctggc
     1861 gggtcaaacg gcgaaagccg ctgccgatga cggactgttc ccaccgattt ttgcccgtgt
     1921 aaataaagcg ggtacgccag tggcggggct gattatcgtc ggtattttga tgaccatctt
     1981 ccagctcagc agcatttcac caaacgcgac caaagagttc ggtctggttt cttccgtctc
     2041 ggtcatcttt acactggtgc catatcttta cacctgtgcg gcgttactgc tgctcggcca
     2101 cggtcacttt ggtaaagcac gcccggcata tctggctgtt actaccattg ccttcctcta
     2161 ctgcatttgg gccgtggtgg ggtccggggc gaaagaggtt atgtggtcat ttgtcaccct
     2221 gatggtcatc accgccatgt atgcgctgaa ttacaaccgg ctacataaaa acccgtatcc
     2281 cttagatgca ccaataagca aagattaatt ccccttaatc cagcaaacat aaaagccaac
     2341 cttaagaact taaggttggc ttaattttgc tttgcgagca tatgcgcact ttgttcgatg
     2401 gaaacaccgt gatgttgaag cgcctactaa aaagaccctc tttgaattta ctcgcctggc
     2461 tattgttggc cgctttttat atctctatct gcctgaatat tgcctttttt aaacaggtgt
     2521 tgcaggcgct gccgctggat tcgctgcata acgtactggt tttcttgtcg atgccggttg
     2581 tcgctttcag cgtgattaat attgtcctga cactaagctc tttcttatgg cttaatcgac
     2641 cactggcctg cctgtttatt ctgattggcg cggctgcaca atatttcata atgacttacg
     2701 gcatcgtcat cgaccgctcg atgattgcca atattattga taccactcca gcagaaagtt
     2761 atgcgctgat gacaccgcaa atgttattaa cgctgggatt cagcggcgtg cttgctgagc
     2821 tgattgcctg ctggctaaaa atcaaacctg ccacctcgcg tctgcgcagt gttcttttcc
     2881 gtggagccaa tattctggtt tctgtactgc tgattttgct ggtcgccgca ctgttttata
     2941 aagactacgc ctcgttgttc cgcaataaca aagagctggt gaaatcctta agcccctcta
     3001 acagcattgt tgccagctgg tcatggtact cccatcagcg actggcaaat ctgccgctgg
     3061 tgcgaattgg tgaagacgcg caccgcaacc cgttaatgca gaacgaaaaa cgtaaaaatt
     3121 tgaccatcct gattgtcggc gaaacctcgc gggcggagaa cttctccctc aacggctacc
     3181 cgcgtgaaac taacccgcgg ctggcgaaag ataacgtggt ctatttccct aataccgcat
     3241 cttgcggcac ggcaacggca gtttcagtac cgtgcatgtt ctcggatatg ccgcgtgagc
     3301 actacaaaga agagctggca cagcaccagg aaggcgtgct ggatatcatt cagcgagcgg
     3361 gcatcaacgt gctgtggaat gacaacgatg gcggctgtaa aggtgcctgc gaccgcgtgc
     3421 ctcaccagaa cgtcaccgcg ctgaatctgc ctgatcagtg catcaacggc gaatgctatg
     3481 acgaagtgct gttccacggg ctggaagagt acatcaataa cctgcagggt gatggcgtga
     3541 ttgtcttaca caccatcggc agccacggtc cgacctatta caaccgctat ccgccgcagt
     3601 tcaggaaatt taccccaacc tgcgacacca atgagatcca gacctgtacc aaagagcaac
     3661 tggtgaacac ttacgacaac acgctggttt acgtcgacta tattgttgat aaagcgatta
     3721 atctgcttaa agaacatcag gataaattta ccaccagcct ggtttatctt tctgaccacg
     3781 gtgaatcgtt aggtgaaaat ggcatctatc tgcacggtct gccttatgcc atcgccccgg
     3841 atagccaaaa acaggtgcca atgctgctgt ggctgtcgga ggattatcaa aagcggtatc
     3901 aggttgacca gaactgcctg caaaaacagg cgcaaacgca acactattca caagacaatt
     3961 tattctccac gctattggga ttaactggcg ttgagacgaa gtattaccag gccgcggatg
     4021 atattctgca aacttgcagg agagtgagta aatgaaaatt ctgattgttg aagacgatac
     4081 gctgttattg cagggactga ttctggcggc gcaaaccgaa ggctacgcgt gcgatggcgt
     4141 gacaaccgcg cggatggcgg aacaaagcct tgaggacggt cattacagcc tggtggtact
     4201 ggatttaggg ttacccgacg aagatggact gcattttctc gcccgtatcc ggcagaaaaa
     4261 atacaccctg ccggtactga tcctcaccgc tcgcgatacg ctgaccgaca aaatcgccgg
     4321 gctggatgtc ggtgccgatg actatctggt gaagcctttt gcgctggaag agttacacgc
     4381 ccgtatccgc gccctgctac gacgccataa taatcagggc gaaagtgagc tgattgttgg
     4441 caatctgacg ctgaatatgg gtcgccgtca ggtatggatg ggcggtgaag agttgattct
     4501 gacgcccaaa gaatatgctc tgctgtcacg gttaatgctc aaagcaggca gtccggtaca
     4561 tcgggaaatt ctctacaacg acatctataa ctgggacaat gaaccctcga ccaacaccct
     4621 ggaagtgcat atccacaatc tgcgcgacaa agtgggcaaa gcccgtatcc gcaccgtgcg
     4681 cggctttggc tatatgctgg tcgcgaatga ggaaaactaa ttgaatctga tgcgttttct
     4741 gcgccgacct atatcgctgc gccaacggct gatattgacc atcggggcca ttttgttggt
     4801 gtttgagctg atcagcgtct tctggctatg gcatgaaagt accgagcaga ttcagctgtt
     4861 tgagcaggcg ctacgcgaca atcgcaacaa cgatcgccac atcatgcgtg agatccgcga
     4921 ggcggtcgcc agcctgattg tccccggcgt ctttatggtc agcctgacgc tatttatctg
     4981 ctatcaggcg gtacgccgca tcacccgccc gctggcggag ctgcaaaaag agctggaagc
     5041 gcgcaccgcc gacaacttaa cgcccattgc cattcacagc gccaccctcg aaatcgaagc
     5101 ggtggtttcg gcgttgaacg atctggtcag tcgcctgacc agcacgctgg ataacgaaag
     5161 gttgtttacc gctgacgtcg cgcacgaact gcgaacgcca ctggcggggg tgcgtttgca
     5221 tctggaactg ctggcgaaaa cgcatcacat tgatgtagca ccgttagtgg cacggcttga
     5281 tcagatgatg gagagcgtct cccagctgct gcaactggcg cgtgccggac agtcattttc
     5341 ttccggtaat tatcaacatg taaaactgct ggaagatgtc attctcccct cgtatgacga
     5401 actcagtacc atgctcgacc agcgacagca aaccctgcta ttgccagaga gcgccgccga
     5461 catcaccgtt cagggtgatg caaccctgct gcggatgtta ttgcgaaacc tggtagaaaa
     5521 cgcccatcgt tacagcccgc aaggcagcaa cattatgatt aagctgcaag aagatggcgg
     5581 agcggtcatg gcagttgaag atgaaggacc aggtattgat gagagtaaat gcggggagtt
     5641 gagtaaagcg tttgtacgta tggacagccg ttatggcggg attggtctgg ggttaagtat
     5701 tgtcagccgc attacacagt tgcatcacgg gcagtttttc ctgcaaaacc ggcaagagac
     5761 ttccggcacg cgggcctggg tacggctgaa gaaagatcag aacgtggcaa accagatata
     5821 aagaaagctg ctgaccacca gcacgctgaa cacggtagtt aaactttcat aaacacggtt
     5881 tttcattgca ctctcctccg ttaacctgga ggagagtatg cgcgtcagag attaagtgaa
     5941 ccttaagagt tcaattggcc tgataagaca gcgtcacatc aggccatccg tttcagctta
     6001 ttcatcaatt cgcggatgtt gctgcaccag gcgggtacgt ttcgcctgca aatcggcaat
     6061 ctcgtggtca atattatcga ttttctgctc gatattatcg taatgctcga cgagaatttc
     6121 cttcgcttcc tgtatatctg acgccgccgg tgtcgcacct ttcaacggac gatttgccgt
     6181 ctctttcatg gttacgccgg tgattaaacc aatcaccgcc actaccatca ggtaataggc
     6241 aggcatcatc agattctgcg agctttcgac cagccaggcc gccagtgttg gcgtcagacc
     6301 ggcaaccagc accgaaatat taaatgccgc cgccagcgcg ctgtaacgga tatgcgtcgg
     6361 gaacatcgct ggcaaggtag aagccataac gcccataaag cagttaagga tcaccgccag
     6421 catcagtaac ccggcaaaaa tcaggccgat gacgttactg ttaatcagaa taaacgccgg
     6481 gatcgccaac acaaacagtg caacactacc aagtagcaca aacggacgac ggccaaaacg
     6541 gtcactcagc aagcccatca ccggctggac aaacagcata ccgatcataa tggcgataat
     6601 aatcagcacc ccgtggtctt cggagtaatg caggttatgc gacaaataac tcggcatata
     6661 ggtcagcaac atgtagtaag tcacgttggt ggcaattacc agaccaatac atgtcaacag
     6721 gctgcgccag tatttagtgg caatctcttt aaacgagact ttcgggccat cctgcaaacc
     6781 ttcgcggtcg ccctgttcca gtttatcaac atgctgctgg aacgccggag tttcttccag
     6841 cgcatggcgc aggtaaagcc cgataatccc taacggcaga gcaataaaga acggaatacg
     6901 ccagccccag tcgaggaagt tcgcttcgcc gacaatggtc gaaattaaca ccaccacgcc
     6961 cgcacccagc acaaacccgg caatagaacc gaagtccagc cagctgccca taaagccacg
     7021 tttacggtca ggggagtatt ccgcaacaaa tatcgacgcc ccggtatatt caccaccgac
     7081 cgagaaacct tgtgccatct tacagatcaa cagcagaatc ggtgcccaaa taccaatcgt
     7141 gtcgtaggac ggtattaagc caatacagaa cgtactgatc gacataatca caatagtgat
     7201 agcgaggatc ttctggcgac catatttatc gcccaacata ccaaagaaga gtccgccaag
     7261 cggtcgaatc agaaagggaa cggagaaagt ggcaagtgca gcaaccatct gcacgctggg
     7321 gtcagccccc gggaaaaaaa ctttacctaa tgcgtaagca acaaaaccat aaacaccaaa
     7381 atcaaaccat tccattgcat tacccagtga tgctgcggta atggctttac gcagtttacc
     7441 gtcatcaata atggtgacat cacgaagggt aatcggtttt acttttttcc ttttcagcat
     7501 agctttcctc gcagagttgg cccatttccg caaacgccag aaaaatcagc gcgacggccg
     7561 caaaagggga gcgggaattg caggatgcaa tctctgtaac aagcgtagcg ggtttgctta
     7621 caccctccgg ttaaggaatg tacaatcaaa ccagttgacg cctgaattgg gcagttaatg
     7681 acctttttac cctaccagtg tttaaatttg tgatcaactt cacacatgga tttcgtttag
     7741 gactcattga tgtaactgat tatttacgcc aaatttacag agaagatttc ctcgaccatt
     7801 gcccccacca aacgctgcgc tgaacagagt cgtggcatcc ccagcgcgac agtttgccag
     7861 cgttgggtca ctgaccagca aacgggttgt ttatctgccg tggtccactt tcccagccaa
     7921 cttaaaaaat cagcatgatc cccgcccgtc ggaacggtcg aagaggcaaa ccactgatgg
     7981 taatagtgac gcgtagtcgg tgccgcattg atactctctg ccagatgttg tgtcgctttc
     8041 tcgcgcaaat cacccagcaa caacaccacc gcttgcgggc ttcccagacg gcaggcatcg
     8101 ccaaatgcac cccagcgcaa ctcctccaga acgataaaac agcgcccagg caacgaccag
     8161 gcgttatacg ccccagcacg ccagcgggta aaaatttgct cgctctgatc acctgcctgc
     8221 accagtaggc ctcgttgcgt taacgccttt tccttccagc gacaacgggc tgaaaaccac
     8281 tcatacaagg cggcaatttg ctgggggagc tccgggaaag cagagtgctg gcggtgatcc
     8341 attttttgca gcagcgccag atgctgcgcg atctgcgtca gcgccagatg accgggatcc
     8401 agcatcgacg ccagtttttc caccattgtc agtcgcaggc taagcggcgc atttaacatg
     8461 ccgccgttcg tccatgtctg aagctgcaca cgcgaaagaa gttcctgcat cagtcgttca
     8521 cgaaactgct gctgatgggc ttgaggatgg cgggcatcat cgccctgcgc cagatccact
     8581 aaaaagcgcg gatgaaccga ctccagcacg cgaccggggc cgtccagtaa cgtcttggtc
     8641 aatatcgttc tgccgtgaaa agtgtttgaa tatcatcgcg taacagctga gcgtcggtgt
     8701 aaatccagct gtgagtcatc acagtctgct gcaattgctg ctgcatcagc ctgaccaccg
     8761 attcattttg ttgacgcaga gccagacttt cgcgtaaacg cgtctgtaat tctgtcaaac
     8821 atgaagcgaa ctcagcgaaa aaagtattca tgcctgccgt aacagattca tcgacctgct
     8881 ctgccagaac tttagccatt tgttggcaat agagatcgac ttctgcgctt aatgctcgtt
     8941 gcaacacact gtaatcaacc gtttctgtcg gggatttctc atttccccgt ccccagtcgg
     9001 gctgattcaa ccagcgcgaa aaagtctcac gcacaacgcc taaacgcgtg ctctgctcgt
     9061 ccgttgcttc ctggcgcgaa atgacttcac tgaacagctg gcgagtgttg aagtggggaa
     9121 ctacgccgtg aaaaacagga aaatgaaacc caggacgaaa ccctgactcg ctcaattcca
     9181 ttttgacttg ttgctcaatg gggcgaataa catcggttaa cacccggcaa agggtggatt
     9241 ccatctcggc aaaacgcagc gtaaagtcgc gactgatggt gttctgcgcc gtctgtaaca
     9301 gtgtctcaca gcgggtacga atctcgctta acggctccga atcatcctga aacaaggcgg
     9361 ctaactgcgc attcaccgca tcttgctgtt gacgcagaaa gtggttggcg gaggtcaggg
     9421 ccagctcgat ttcatgttta atctcgccgc tcacctgcgc ctgattgagt tgcaatagct
     9481 gcaaactttc ttcgacctga tggatatttt gccgcaattg ttcacaagcg acgtttaacc
     9541 cgtgcgcacg aaaatccagg tattcccgcg cctgctgcgc gtaattcaac agtttatgcg
     9601 cagcagatcg cagagcatac aatgaggcgt tagcgtaagc ggcatgaagc aacgcctgaa
     9661 ttggctgggc gaacagcgaa tcttcccaca actgatcggc agcatgacga atatgttcga
     9721 ggtccgccag atcggcatga cgccagcgcc tgccgagcgc ggcatgggca aaatcttcca
     9781 cccagcgttg ttgctctggc gctggtaact taccgttgtt ggctaactca tagcgcgccc
     9841 gattcgccag gtagccccac atcgacgaca ccgggaatat ctgctgtggc gtaatacagc
     9901 ctttcatcag cgtcccggaa atcagtgccc gcacctggtc ggcgtcgtca ctgttacgat
     9961 cctgttgatc gaacttattg accagcacat acagcggcac cgattgcccc accgccaaaa
    10021 tcgcctcacg gacctcttca tcggagatcg atttcagttg cgtataatcc agcaccgcca
    10081 gtaccgccga ggcgcgtgcc agctgctggt taagcatttt ttgcagatgc ggttgcccgg
    10141 cttcatttgg cccgggggta tccagtaacg tcaactgacc gggataactt tccagccccg
    10201 ccagatggac aaactccact tcaatcacgg gaatatgctc aatggcggcg taagcagaaa
    10261 aaggaaaatc gacgtccagc gccttcgcca gtcgcactaa atcattcaaa cttttcagac
    10321 aatgaaaaat aggctgggca cccagataat atttttcgaa agcgacgcca ttttcgatcc
    10381 gctgcataag cgcacgcata tctttatcta tttccagcac atcggtcaga tgcttaatat
    10441 cgcaatcacg caggcgctgt tgtaattgtt gaattaaaca atcgattggc gcgacatgtg
    10501 aaaaatgcag taccggttcc ttttgcccgg gcgtatggcg aataagcgtc ggcagcgcag
    10561 tcattgggcg attacgatta ggcagaacct ccgtaccaac aatggcatta atggtggttg
    10621 atttccctgc tttcatggta ccgacaattg ccagcaccat ttccagtcgg gaaattttac
    10681 acaactcatt attcagcatc gcgtgacgtt cggcgatatt aggctgactc cagggtaaag
    10741 ccagttgtgg cgcgtcgtct ccgggtacag agagaggcat tttttccagt aactgcaact
    10801 gttggcgaga aagctgtaac aggcgttcag cctcctgact taactcatac agggtctgtg
    10861 tgtacataga aaattcttcc ttaaagcaaa ttttgttact tttatttagc cagattgttt
    10921 ttgagttctg ttttcggctt ttataattac tgcaagaaat aattttatat tta
//
LOCUS       DAZPCS010000125        10878 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZPCS010000125 DAZPCS010000000
VERSION     DAZPCS010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10878
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.125"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..794
                     /locus_tag="ACHUQ7_003057"
     CDS             <1..794
                     /locus_tag="ACHUQ7_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="shiE"
                     /protein_id="HFR1136361.1"
                     /translation="SETKAAYRCFSNDKVSANKIMTPHKENITERAQQFKRVLVLQDT
                     TELNYSGQKEKQGVGPKKHKDERNLFLHPQLVISESGVCLGVYDDYQWFRDELKTSKN
                     TRQEICNDLLHKKHVSEKETWRWVEGYNKATELARQCPDTHVLSISDREGDFYDLFER
                     AAQTPGIKADWLVRMKFNNRATLNINGKRDHRLLHERIMEITPQQLVEFTIPDGRGQQ
                     ARTLPDMLSSDAGPLSYPLIYSSKVMIISLFDSTVMPDKYRHNGK"
     gene            complement(744..1937)
                     /locus_tag="ACHUQ7_003058"
     CDS             complement(744..1937)
                     /locus_tag="ACHUQ7_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1136362.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2073..3797
                     /gene="iucA"
                     /locus_tag="ACHUQ7_003059"
     CDS             2073..3797
                     /gene="iucA"
                     /locus_tag="ACHUQ7_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HFR1136363.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3798..4745
                     /gene="iucB"
                     /locus_tag="ACHUQ7_003060"
     CDS             3798..4745
                     /gene="iucB"
                     /locus_tag="ACHUQ7_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase IucB"
                     /protein_id="HFR1136364.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4745..6487
                     /gene="iucC"
                     /locus_tag="ACHUQ7_003061"
     CDS             4745..6487
                     /gene="iucC"
                     /locus_tag="ACHUQ7_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIS family aerobactin synthetase IucC"
                     /protein_id="HFR1136365.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6484..7821
                     /gene="iucD"
                     /locus_tag="ACHUQ7_003062"
     CDS             6484..7821
                     /gene="iucD"
                     /locus_tag="ACHUQ7_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase
                     IucD"
                     /protein_id="HFR1136366.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            7827..10022
                     /gene="iutA"
                     /locus_tag="ACHUQ7_003063"
     CDS             7827..10022
                     /gene="iutA"
                     /locus_tag="ACHUQ7_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HFR1136367.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IATGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(10573..10716)
                     /locus_tag="ACHUQ7_003064"
     CDS             complement(10573..10716)
                     /locus_tag="ACHUQ7_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1136368.1"
                     /translation="MEHRSVIHDELAIDHDAILQCAEEVLHHCVIQTVSFVRHILCYS
                     MGL"
BASE COUNT         2704 a         2748 c         2840 g         2586 t
ORIGIN      
        1 ggagtgaaac aaaggcagct tatcgttgtt tttcaaacga taaagtctct gctaataaaa
       61 tcatgactcc tcacaaagaa aatatcactg aaagagctca gcagtttaaa agagttttag
      121 ttttacagga tacaacagaa cttaattatt ctggtcagaa agaaaaacaa ggagttggtc
      181 ccaaaaaaca taaagatgaa agaaacttat ttcttcatcc tcaactcgtc atcagtgagt
      241 caggtgtttg cctcggtgtt tatgatgact atcagtggtt cagggatgaa ttaaaaacca
      301 gtaaaaatac ccgacaagaa atctgcaatg atttacttca taaaaaacat gtcagtgaaa
      361 aagaaacctg gcgttgggtt gaaggatata ataaagcaac agaactggct cgccaatgcc
      421 ctgataccca tgtattgtca atttctgatc gtgaaggcga tttttatgac ctgtttgaac
      481 gcgcagcgca gactcccgga ataaaagctg actggcttgt cagaatgaaa tttaataaca
      541 gagcaacgct gaatattaat ggtaaacggg atcataggtt gttgcacgaa agaatcatgg
      601 agattacacc gcagcaactg gttgaattca ctatacctga tgggcggggg cagcaagcca
      661 gaacactgcc tgacatgctt tcatcagacg caggtcctct ctcatatcct ctgatttact
      721 ccagtaaggt gatgatcata tctttatttg atagtacggt aatgccagat aaatacagac
      781 acaatggtaa ataatatagt tgcaaataat gctgccataa tcagcgcgat tgcgacacct
      841 gttttcgata ccagcggcac cagcaaagcg cacagtccat agccaagtgt gtgactggta
      901 gcgagccagc ccgcgcctgc gccatcagcc agcttatcat tcagtagaag ttgataagcg
      961 ggtgttgcca gagctgctcc aaatgacagc actgcacagc ccgggtaaaa caaccatagc
     1021 tgttcggaga gcatgatagc cagaccacca ctcatcaaca ctccggcact caggagcagg
     1081 gccaccggag tcaggcgctg cggacggagt accccgaact gcgcgataag cgcagctact
     1141 gcggacagac ccaacagcca ggccacctgt tggctaatgg cggtggtatc cgtagcgaac
     1201 tggcgagtaa gggcaggcga aagtccaagc tgcatcatgc tcaccgccgc agccagtaat
     1261 attgcgcaaa gcagataagg cagacaatcc cgcttcagac tgacgctctt gcactccggt
     1321 gtgggctgtg gtggtgtgcc gggcaaccgc agcagcatca gcagcgccag cactggggct
     1381 gccatcagca gccccagtgg cgccagagga tgaatggcca gcattgctgc cgcgcacagc
     1441 ggcccgaata gcctgccgca actcaggccg gagctgatgg ttgccagagc ggccatgcga
     1501 ttcccttctc cagctctctg caatgcccag acctgacagg ctggcaccat tgccgacacg
     1561 gtcagcccgt aggcgatccg ggcgatgatt aatattccca gtcccaccgc tgttgttatg
     1621 gcgctggtag ccatcagcac gcttcccagc cctagcaggg taaagcttat gccatatcct
     1681 cccagagccc acaacaccac aggtttactg ccagtacggg aaatttgctt tccccaccac
     1741 ggggacgatg gcagaaacag cattgagcca agcatcagca gggcagccca tacagacaga
     1801 ctcagatttg tctgtatgac cagaacaggg agcacaacca gcagaccgtt ctgtccgata
     1861 ccgagaagcc cggcactgaa cgcaagtggc cagcaggaca gtggtttttg gggcgtatct
     1921 tcgatccctg gtgacattat aatgtttcaa ctccatgtat taattgtgtt tatttgtaaa
     1981 attaatttat ctgacaataa catttattgt tgataatgag aatcattatt gacataattg
     2041 ttattatttt actgtgtggg agctgtttga ctatgaccct gccctctgaa aaaccagcca
     2101 cagatgtggc tgcgcagtgc ttcctgaatg cactgattcg tgaaaccaca gactggaaac
     2161 tgacagaata cccgccagac gaattgctca tcccgctgga tgagcagaaa tcgctccatt
     2221 tcagagtggc ttatttctcc ccaacccagc atcaccgctt tgcatttcct gcacgtctgg
     2281 tcacggcatc aggcagttat cctgtcgact ttaccactct ctcccggctt attattgata
     2341 agctacgcca tcaactgttt ctgcccgttc ccctctgcga aactttccac cagcgcgtgc
     2401 tggaaagcca tgtccatacg caacaggcaa ttgatgcccg tcatgactgg gccgctctgc
     2461 gtgaaaaagc gttgaatttt ggcgaggctg agcaggcgct gctgacagga cacgctttcc
     2521 accctgcgcc taagtctcat gaaccgttta accggcggga ggctgaacgc tacctgcctg
     2581 atatggcacc ccacttccca ctgcggtggt tttcggtgga taaaacgcaa atcgccggtg
     2641 aaagtttgca tctcaacctt caacagcggc tgacgcgatt tgccgcagag aatgcgcctc
     2701 agttactcaa cgaattaagt gacaaccaat ggctgttccc gttgcacccg tggcaggggg
     2761 aatatctttt gcagcagggg tggtgccagg cacttgttgc taaagggctg attaaagact
     2821 taggtgaggc cggcacgtcg tggctgccga ccacctcttc ccgttccctc tactgtgcca
     2881 ccagccgcga tatgatcaag ttctccctga gcgtacggct gaccaactcc atccgtactc
     2941 tgtccgtgaa agaagtgaag cgtgggatgc gcctggcacg cctggctcaa accgacggct
     3001 ggcagatgct acaggtccgc ttcccgactt tccgggtaat gcaggaggat ggctgggccg
     3061 ggctgctcga tcttaacggc aacatcatgc aggaaagtct gtttgccctg cgtgaaaatc
     3121 tgctggtgga tcagccgaaa agccagacta acgtactggt ctccctgact caggccgcac
     3181 ctgatggcgg tgattcgctg ctggtttcgg cggtaaaacg cctgagcgat cgcctcggta
     3241 tcactgtgca acaggccgcc catgcatggg ttgatgcata ctgtcagcaa gtgctaaagc
     3301 cgctgtttac ggctgaagcg gattacggcc tggtgctgct ggcgcatcag caaaatattc
     3361 ttgtccagat gcttggggat ctgccggtcg gatttattta ccgtgactgt cagggcagcg
     3421 cttttatgcc tcacgcgaca gactggctcg attctattgg cgaggcgcag gcggaaaata
     3481 ttttcaccca tgagcagttg ctgcgctatt tcccttatta cctgctggtt aactccactt
     3541 ttgctgtgac cgctgcgctg ggggctgccg ggctggacag cgaatcgaat ctgatggctc
     3601 gtgtacgagc atcgctggct gaagtgcgtg atcaggtgac tcataaaacc tgtctcaact
     3661 acgtgctgga aagtccgtac tggaacgtaa aaggtaactt tttctgttat ctgaacgatc
     3721 ataacgagaa caccattgtt gacccttcgg tgatctattt cgatttcgct aacccgctgc
     3781 aggctcagga ggtctgaatg tctgaggcaa acattattca cagcagatat ggactgcgct
     3841 gtgaaaaact cgacaagccc ctgaatcttg gctggggact ggacaatagc gcggtgttgc
     3901 actgtcccgg ggagctgccg acagggtggc tgtgcgatgc gctggatcag atatttatcg
     3961 ccgcaccaca actgtcagca gtggctctcc cctgggccga atggcgtgag gagccacagg
     4021 cgctgacgct ttttgggcag gtaaaaagcg acattatcca tcgtactgcc ttctggcagt
     4081 taccgttatg gttgagttca ccggcaaacc gggcctccgg tgaaatggtt tttgatgcag
     4141 agcgtgagat ttatttcccg cagcgcccac cccgtccaca gggtgaggtt taccgccgtt
     4201 acgatccacg tattcgcagg atgctgagtt tccgcattgc cgatcccgtt tctgatgcag
     4261 aacgtttcac ccgctggatg aatgatccgc gcgttgagta tttctgggag caaagtggct
     4321 cactggaggt acagaccgcc tatctggagc gccagctcac cggtaaacat gcattcccgc
     4381 tgattgggtg cttcgacgat cggccattca gctatttcga aatctactgg gcggcggaag
     4441 accgcattgg tcgtcactat tcgtggcagc cctttgaccg tggcctgcat ctgctggttg
     4501 gtgaacagca atggcgcggg gcccactatg tgcaaagctg gctgcgcggg ctgacacatt
     4561 acctgctgct ggatgagccc cgtacgcagc gcacagtact ggagccacgc accgataacc
     4621 agcgcctgtt ccgccatctt gagcctgcgg gataccggac aattaaagag ttcgacttcc
     4681 cacataagcg ctcgcgcatg gtgatggcgg atcgtcatca cttcttcacg gaggtcggtc
     4741 tgtaatgaat cacaaggact gggattttgt taaccgtcgg ctggtggcaa aaatgttgtc
     4801 tgagatggag tatgagcagg ttttccacgc ggaatctcag ggcgatgacc actactgcat
     4861 taacctgccg ggagcacaat ggcgcttcat cgctgaacgt ggtatctggg gctggctgtg
     4921 gattgatgcc caaacgttgc gctgcacgga cgaaccagta ctggcgcaga cgctgctgat
     4981 gcagctaaag ccggtgctgt caatgagtga tgccactgtt gcagagcata tgcaggattt
     5041 gtatgccacg ctactgggcg acctgcaact gctgaaggcc cgtcgcggac tgagtgccag
     5101 tgacctgatt aatcttgatg ccgatcgtct gcaatgcctg ctgagcggtc atcctaaatt
     5161 cgtttttaat aaaggtcgcc gtggctgggg caaagaggcg ctggaacgat atgcgccaga
     5221 gtataccaac accttcagac tgcactggct ggcggtaaaa cgtgaacata tgatctggcg
     5281 ctgcgataac gatctggata ttcagcagtt gttgactgcc gcaatggatc cgcaggagtt
     5341 tacacgcttc agtcaggtct ggcaggaaaa cgggctggat cacaactggc tgccgctgcc
     5401 ggtacatccg tggcagtggc agcaaaaaat cgccacagac ttcatcgctg attttgccga
     5461 aggcaggatg gtgtctctcg gcgagtttgg cgaccagtgg ctcgcccagc agtcgctgcg
     5521 taccctgacc aacgccagcc gccggggagg gctggatatc aagctgccgc tgaccatcta
     5581 caatacctca tgctaccggg ggatccctgg cagatatatc gctgccggac cactggcttc
     5641 acgctggctg caacaggttt ttgcgaccga cgccaccctg gtgcaaagcg gcgcagtgat
     5701 ccttggtgaa ccggctgcag gctatgtgtc ccatgaaggc tatgccgcgc ttgcccgggc
     5761 tccctatcgc taccaggaaa tgcttggtgt tatctggcgg gagaatccgt gccgctggct
     5821 gaaaccggat gaaagtcctg ttctgatggc aacactgatg gagtgcgacg aaaacaatca
     5881 gccgctggca ggcgcatata tcgaccgctc cggactggac gctgaaacct ggcttacgca
     5941 attgttccgg gtggttgtgg ttccgctgta tcacctgctt tgccgctacg gtgtcgcgct
     6001 tattgcacac ggacaaaata taacgctcgc catgaaagag ggggtaccac agcgtgttct
     6061 gctgaaagac ttccagggcg atatgcggct ggtgaaagaa gcgtttcccg aaatggactc
     6121 tttgcctcag gaggtgcgtg atgtcacatc ccgtctgagt gcggactact taatccatga
     6181 tttgcagacg ggtcacttcg tgacagtact gcgttttatt tcgccactga tggttcgtct
     6241 tggcgtacct gaaaggcgat tttatcaact gctggcagca gtgttgagtg attacatgaa
     6301 caaacatcca caaatggcag agcgttttgc gcttttctca ctcttcaggc cacaaatcat
     6361 tcgcgtggtg ctgaacccgg taaaactgac ctggccggac ctggatggcg gcagtcgcat
     6421 gctgccgaat taccttgaga atctgcaaaa tccgctgtgg ctggtaactc aggaatatga
     6481 atcatgaaaa aaagtgtcga ttttattggt gtcgggacag ggccatttaa tctcagcatt
     6541 gctgcattgt cacatcagat cgaagaactg aactgtctct tctttgacga acatcctcat
     6601 ttttcctggc atccgggtat gctggtaccg gattgtcata tgcagaccgt ctttctgaaa
     6661 gatctggtca gtgcagttgc acctacaaat ccctacagct ttgttaacta tctggtgaag
     6721 cacaaaaagt tctatcgctt ccttacaagc aggctacgta cagtatcccg tgaagagttt
     6781 tctgactatc tccgctgggc tgctgaagat atgaataacc tgtatttcag tcataccgtt
     6841 gaaaatattg atttcgacaa aaaaagtcga ttgtttctgg ttcagaccag tcggggagaa
     6901 tattttgccc acaatatctg ccttggtaca ggaaaacaac cttatttacc accctgtgtg
     6961 aagcatgtga cacaatcctg tttccatgcc agtgaaatga atcttcgtcg gccggacctg
     7021 agtggaaaac ggataaccgt ggttggtgga ggacagagtg gtgcagacct gttccttaat
     7081 gcattacgcg gggaatgggg agaagcggcg gaaataaact gggtctcacg gcgtaataat
     7141 tttaacgcac tggatgaggc tgcttttgct gatgagtatt ttacacctga atatatttca
     7201 ggcttctccg gactgaagga agatattcgc catcagttac tggatgagca gaaaatgaca
     7261 tcggatggca tcactgccga ttctttactg accatttatc gtgagttgta ccaccgtttt
     7321 gaagttctga gaaaaccaag aaatatccgt ctgctaccca gtcgctcggt aacaactctg
     7381 gaaagtagtg gtccgggctg gaggttattg atggaacatc atctggatca gggcagagag
     7441 agcctggaaa gtgatgtggt gattttcgcc acaggttacc gttctgcatt gccacaaata
     7501 cttccctcac tgatgccctt gatcaccatg cacgataaga acacctttaa agtgcgtgat
     7561 gacttcactc tggaatggag tggcccgaaa gaaaataaca tctttgcggt caacgccagc
     7621 atgcaaactc atggcatcgc cgaaccccag ctcagcctga tggcctggcg atctgcacgt
     7681 attcttaatc gcgtactggg gcgtgattta ttcgatctca gtatgccgcc cgcactgatt
     7741 cagtggcgca gcggcagccg gaaaaaacca cagccggagg ctgcttcttt aactcactat
     7801 acagcaaata ttcaggaata acgatgatgc gcaaaaagta tatgccccgg gctcttggtc
     7861 cgctgcttct tgtcgtgctg tcaccagctg tcgcccagca aaacgatgat aatgagatca
     7921 tagtgtctgc cagccgcagc aatcgaactg tagcggagat ggcgcaaacc acctgggtta
     7981 tcgaaaatgc cgaactggag cagcagattc agggcggtaa agagctgaaa gacgcactgg
     8041 ctcagttaat ccccggcctt gatgtcagca gccagagtcg aaccaactac ggtatgaaca
     8101 tgcgtggccg cccgctggtt gtcctgattg acggtgtgcg cctcaactct tcacgttccg
     8161 acagccgaca actggactct gtcgatcctt ttaatatcga ccatattgaa gtgatctccg
     8221 gcgcgacggc cctgtacggt ggcgggagta ccggagggtt gatcaacatc gtgaccaaaa
     8281 aaggtcagcc ggaaaccatg atggagtttg aggctggcac aaaaagtggc tttaacagca
     8341 gtaaagatca cgatgagcgc attgccggtg ctgtctccgg cggaaatgac catatctccg
     8401 gacgtctttc cgtggcatat cagaaatttg gcggctggtt tgacggtaac ggcgatgcca
     8461 ccctgcttga taacacccag accggcctgc agcactccaa tcggctggac atcatgggaa
     8521 ccggtacgct gaacatcgat gaatcccggc agcttcaact gataacgcag tactataaaa
     8581 gtcagggaga cgacaattac gggcttaatc tcgggaaagg cttttccgcc atcagcggga
     8641 gcagcacacc atacgtcagt aaggggctga attctgaccg cattcccggc actgagcggc
     8701 atttgatcag cctgcagtac tctgacagtg atttcctggg acaggaactg gtcggtcagg
     8761 tttactaccg cgatgagtcg ttgcggtact acccgttccc gacggtaaat gcgaataaac
     8821 aggcgacggc tttctcctcg tcacagcagg ataccgacca gtacggcatg aaactgactc
     8881 tgaacagcca actgatggac ggctggcaaa tcacctgggg gctggatgct gagcatgagc
     8941 gctttacctc caaccagatg ttcttcgatc tggctcaggc aagtgcttcc ggagggctga
     9001 acaaccataa gatttacacc accgggcgct atccgtcata tgacatcacc aatctggcgg
     9061 ccttcctgca atccagctat gacattaatg atatttttac cgttagcggt ggcgtacgct
     9121 atcagtatac tgagaacagg gtagatgatt tcatcgacta cacgcagcaa cagaagattg
     9181 ctaccgggaa ggcgatatct gccgacgcca ttcctggtgg ttcggtagat tacgataact
     9241 ttctgttcaa tgctggtctg ctgatgcaca tcaccgaacg tcagcaggca tggttcaatt
     9301 tttcccaggg ggtggcattg ccggatccgg ggaaatatta tggtcgcggc atctatggtg
     9361 cagcagtgaa cggccatctt cccctgacaa agagcgtgaa cgtcagcgac agtaagctgg
     9421 aaggcgtgaa agtcgattct tatgaactgg gctggcgctt tatcggtgac aacctgcgga
     9481 ctcaaatcgc ggcatattac tcgctttcca ataagagcgt ggaaaggaat aaagatctga
     9541 ccatcagtgt gaaggacgac aggcgccgta tttacggcgt ggaaggtgcg gtggactacc
     9601 tgatcccgga tactgactgg agtaccggtg tgaacttcaa tgtgctgaaa accgagtcga
     9661 aagtgaacgg tcaatggcaa aaatatgacg tgaaggaatc aagtccatcg aaagcgacag
     9721 cttacattaa ctgggcgccg gaaccgtgga gtctgcgtgt acagagcacc acttctttcg
     9781 acgtaagcga tgcagagggt aacgatatta atggttacac taccgtcgat tttatcagta
     9841 gttggcagct tccggtggga acactcagct tcagcgttga gaacctcttc gaccgtgact
     9901 ataccactgt ctggggacag cgtgcacctc tgtactacag cccgggttac ggccctgctt
     9961 cactgtacga ctacaaaggc cggggccgaa cctttggtct gaactactca gtgctgttct
    10021 gacggggatg cttttgcaac aagggtatgc tgtaaacaac atggtccttg gtggccatgt
    10081 tgtttacagc cagtataata tacagcctta acaagatatt ccgtctccgg aatgggctgt
    10141 tcaccactga ataacattat gtctgtttta tggatctttg actgaaattt atcgcatatc
    10201 ctcatttcat tgtccgtctg aaaactaacc ttacaagtaa gcgtcgtctc agcaccgtct
    10261 ggcagatcct gaaattcctg agaggatgat ctgcgcccaa tatcctgagc cggaactttg
    10321 ctgcaatgaa ggccaacgaa aaatggggga gtatgttata gagtacttgc tacagggtaa
    10381 aaagaggtac ctgtcgctgg ttctccgttc agtttaaccg ggagattttc tcctgcgcct
    10441 gtcataaagt ccggtaatgg agattgttaa tacgatggtg cacgatgcat cgtcagtgcc
    10501 cggcccggag gatgccccgt agctacatac ggatcagggt tggcaatatc gaatggcagg
    10561 ctataggcaa agttaaagcc ccatggagta gcacaaaata tgccgcacaa aggaaacggt
    10621 ctgaataacg cagtgatgga gaacttcttc ggcacactgc aaaatcgcat cgtggtcgat
    10681 cgctaattcg tcgtgaatca ctgaacgatg ttccatgcga acagatattc gatcatatcg
    10741 aaatattaaa cattacccct ccccacattg ggcaaaaaaa aacaaaaaac gatcaattag
    10801 tattgattca cataatctct catcattaat gagaacattg tcacatattt acaatataga
    10861 actgttactt ttagcaag
//
LOCUS       DAZPCS010000126        10771 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZPCS010000126 DAZPCS010000000
VERSION     DAZPCS010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10771
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.126"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            137..850
                     /locus_tag="ACHUQ7_003065"
     CDS             137..850
                     /locus_tag="ACHUQ7_003065"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR1136369.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPLTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            882..1640
                     /locus_tag="ACHUQ7_003066"
     CDS             882..1640
                     /locus_tag="ACHUQ7_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1136370.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERSLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQVQKAVSLVAIVSDRRSLT"
     gene            1686..3035
                     /locus_tag="ACHUQ7_003067"
     CDS             1686..3035
                     /locus_tag="ACHUQ7_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFR1136371.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFVGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            3035..3859
                     /locus_tag="ACHUQ7_003068"
     CDS             3035..3859
                     /locus_tag="ACHUQ7_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1136372.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYYAPLALDSDICQRLELLISRFPL"
     gene            3871..4431
                     /locus_tag="ACHUQ7_003069"
     CDS             3871..4431
                     /locus_tag="ACHUQ7_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFR1136373.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            4462..5532
                     /gene="lptF"
                     /locus_tag="ACHUQ7_003070"
     CDS             4462..5532
                     /gene="lptF"
                     /locus_tag="ACHUQ7_003070"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFR1136374.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRAGSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            5529..6608
                     /gene="lptG"
                     /locus_tag="ACHUQ7_003071"
     CDS             5529..6608
                     /gene="lptG"
                     /locus_tag="ACHUQ7_003071"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFR1136375.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYHLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(6644..7815)
                     /locus_tag="ACHUQ7_003072"
                     /pseudo
     CDS             complement(6644..7815)
                     /locus_tag="ACHUQ7_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            complement(7815..8063)
                     /locus_tag="ACHUQ7_003073"
     CDS             complement(7815..8063)
                     /locus_tag="ACHUQ7_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFR1136376.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(8096..9010)
                     /locus_tag="ACHUQ7_003074"
     CDS             complement(8096..9010)
                     /locus_tag="ACHUQ7_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFR1136377.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVRQLAAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            complement(9007..10695)
                     /locus_tag="ACHUQ7_003075"
     CDS             complement(9007..10695)
                     /locus_tag="ACHUQ7_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HFR1136378.1"
                     /translation="MRYADFPTLVDALDYAALSRAGMNFYDRRCQLEDQLEYQTLKTR
                     AEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAVPLAIPMGVGQRDSW
                     SAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWFKALPEADVALQRPV
                     PNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPSDRCVSWQPFYHDMG
                     LVGFLLPPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVAPPFGYELCQRRVNE
                     KDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFMPCYGLAENALAVSF
                     SDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALPEHGIEIRNEAGMPV
                     AERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLLDGYLYVTGRIKDLI
                     IIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCRISDEERRGQLIHAL
                     AARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYAASLNVQESLA"
BASE COUNT         2594 a         2666 c         2817 g         2694 t
ORIGIN      
        1 ttatttggtt aatatgtttt tatgaaagcg taattcaggt caatgtcaca attagtcatt
       61 gtcacaataa ggttgaacgg atattctgag cacggaccac gtaatattca atacattatt
      121 tactgccggg tttttcgtga atcaacgtaa tatgtcaata attaattcca cgccagtgcg
      181 tgttattgcc attgtaggat gtgatggttc aggtaaatcg accctcacgg caagcctggt
      241 aaatgaactg gcagcaagaa tgccaacaga acacatttat ctcgggcaat cgtccgggcg
      301 aattggcgaa tggatttcac agctccctgt tattggcgca ccttgtgggt gttatctgcg
      361 aagtaaagcg gcacatgtac acgaaaagcc cttaactccg cctggcaata ttactgcgct
      421 ggttatctat ctgctttcct gctggcgggc gtacaagttt cgcaaaatgt tgtgtaaaag
      481 ccagcaaggc tatctgctca tcaccgaccg ctacccgcag gttgaagtgc cgggttttcg
      541 ctttgatggc ccgcaattgg caaaaaccac gggcggtaac ggttggataa aaatgttaag
      601 gcagcgcgag ttgaagctgt accaatggat ggcgtcttat ttacccgtat tgttgattcg
      661 tcttggcatt gatgaacaaa ccgcgtttgc gcgtaaacct gaccaccaac tggcagcgtt
      721 acaggaaaaa atcgccgtta cgccgcaact gacattcaat ggcgccaaga tccttgaact
      781 ggatggtcgg caaccagccg atgaaattat gcaagcgtca ctacgcgcaa ttcacgccgc
      841 gctttcctga tcaccaaaat ggatagggga tgatttaacg aatggatgca ctacaaacac
      901 aaactgttaa tagtacaacc gcgccgcagc ccaactacat tccggggctg attgcggtgg
      961 tcgggtgtga tggcaccggt aaatccacgc tgaccaccga cctggtgaaa tcgctgcaac
     1021 aacactggca aaccgagcgc cgctatctgg ggctgctctc cggcgaagac ggcgacaaaa
     1081 tcaaacgatt gccgttggtt ggcgtctggc tggaacggcg attggcggcc aaatcctcga
     1141 aaacccaaag catgaaaacc aaatctccgg cgctatgggc ggcggtgatt atgtactgct
     1201 tctcgctgcg aagaatggcg aatctacgca aggttcagcg actggcgcaa agtggcgttc
     1261 tggtggtcag cgatcgcttc ccgcaggctg aaatttcggg cttttattat gacggaccgg
     1321 ggatcggcgt cgaacgtgcg accgggaaaa tcagcatgtt tctggcgcag cgcgaacgga
     1381 gtttatacca aaaaatggcg caataccgtc ctgaattaat cattcgtttg gggattgata
     1441 ttgatactgc catctcccgc aagcctgacc atgagtatgc cgagctgcag gacaaaatcg
     1501 gcgtcatgtc gacgattggc tataacggca caaaaattct tgagattgat tccagagcgc
     1561 cctacagcga agtgctggaa caggtacaga aggcggtttc tctggttgcc atcgtttctg
     1621 accgccgaag tttaacttaa ggctggcggg atatttttta ctatcaacga taaggtcagg
     1681 ctgaattggc gggttttaac atcaaacatt ggtttgcaga tggcgcgttt cgcaccatta
     1741 ttcgcaatag cgcctggtta ggctccagta atgtcgagag cgccttgctg gggctgttgg
     1801 cgctctcgtg tgccggtaaa gggatgacgc ccgccatgtt tggtgtactg gtgattgtgc
     1861 aatcgtacgc caagtcgatc agcgatttta ttaagtttca gacatggcag ctggtggttc
     1921 agtacggaac gccagccttg accaacaata atccgcagca atttcgcaat gtcgtctcat
     1981 tttccttctc gctggatatc gtcagcggcg cggtggcgat tgtcggcggc attgccttac
     2041 tgccattcct ttcccattca ttaggtctgg atgaccaaag cttttggctg gcggcgctgt
     2101 attgcacgct cattccttca atggcttcct ccacgccgac cggcattctg cgtgcggtag
     2161 atcgctttga tttgattgct gtacagcagg cgacgaaacc ttttctgcgc gcagcgggga
     2221 gcgtcgcagc ctggtatttt gactttggtt ttgtgggttt tgttattgcc tggtacgtgt
     2281 cgaatctggt tggcggcacc atgtactggt ggtttgccgc gcgcgaatta cgccgtagaa
     2341 atatccataa cgccttcaaa ttgaatctgt ttgagtctgc ccgacacatt aaaggcgcgt
     2401 ggagttttgt ctggtcaacc aacattgccc actccatctg gtcggcgcgt aactcgtgca
     2461 gcacagtgtt ggtggggatc gtgttaggac ccgctgccgc cgggttattt aaaatcgcca
     2521 tgacattctt cgacgccgcc ggaacgccag cgggtttgct gggtaaaagt ttttacccag
     2581 aggtgatgcg tttagatccg cgcaccacca gaccgtggtt gttaggtgtg aagtccggtt
     2641 tactggcggg cggaatcggt attctggtgg cgctcgcggt gttgatcgtc ggcaagccgc
     2701 tcatttcgct ggtgtttggc gttaagtatc tcgaagcgta tgacctgatt caggtgatgc
     2761 tgggcgcaat tgtgatttcg atgctgggct tcccgcagga gtcattgcta ttgatggcgg
     2821 gaaaacagcg cgcttttctc gtggcgcaaa ccatcgcctc aatcggctac atcgtattgc
     2881 tgttcatgtt ctgtcatctg tttggtgtgc tgggagcagc gtttgcttac ttcggtgggc
     2941 aatgtctgga cgtggcgctc tcgttaattc caaccttaaa ggcatttttt cagcgccatt
     3001 ccttgcttta taacgcagcc ggagagaaat cctgatgatg agaaaatatt tcccgcttga
     3061 ggcgagcgag cgtctatttg ttgcgattga agaagatgat gtcgttgatg cacaggttag
     3121 tttgccgccg actattgcac taagttgtac caccgaaatc attcatgata attacgcgtt
     3181 atgcctgcaa ttctggctca atggcgttga tcgtcaggaa ttactgcgtt tagttcgcaa
     3241 acaagccaaa ggcgatgagc tgactgctga tgagcgcaaa caatttaaat acatgcgagc
     3301 gcgctacaag cacctgcgtt ttgcgcaacg gctgtattta aagaagcatc aggccggatt
     3361 tttattcggc aaaacgaccg tttttctggg gcgttttcag gacggctttc gtaacggtaa
     3421 gaaaaatatt gtgtcgtatt acggcaacct gttacggatt tatttaagct ctccggtctg
     3481 gtcgttggtg aactattcat tacgccatag ccagttagaa agcgtaagca gttttattgc
     3541 ctatcggcaa aagcaaatgc ataccctgaa agagattatt gccaaaccgc gactaaccgg
     3601 cagggaattt catgatgtgc gaaaaatcat cagccagcag gtttcttatt acgacacctt
     3661 aagatcgctt gatcctgaaa ataaagaggc gttacagatt tcccgtttcc tcgccgccat
     3721 taatggcctg atgggagata aacacgacga catggtggcg gatgatatgg aaaaccgcca
     3781 gtcgtattat gcaccgttgg cgctggatag cgatatttgt cagcgcttag agctgctgat
     3841 ttcgcgtttt ccgctatagc cggagcaatg atgtcatctc gtttgattat tgcgttgatt
     3901 atcatgctct tagccccggg tgtgcaggca cacaatttcg tcactggcaa aacggttacc
     3961 cctgtttata tccaggaagg cggcgagctg cttctgaata gcgacgacga aattcactac
     4021 caaaagtgga acagcacaca attagcggga aaagtccgca ttattcagta tatcgccggg
     4081 cgtaagtcgg cgaagaaaaa gaactcactg ttaattaaag cggttgaagc ggcaaacttt
     4141 ccgcaggatc gttttcagcc caccaccatt gtgaacaccg atgatgccat ctttggtact
     4201 ggctattttg ttgttggcaa aatcgaaaaa aataaacgcc gctatccgtg ggcgcaattc
     4261 gttattgacg gcaatggaca gggacgcgtt gcctggcgct tgccggagca gagttcaacc
     4321 attctggtgc tgaataaagc cgggcaaatt cagtgggcaa aggacggcag cctgacgccg
     4381 gaagaggtag atcatgtcat tgcgttggcg cagaaactga tcaatgaata aaaaactcaa
     4441 aggattgagg gcgttagtga gatgaagctg gttgaacact acatcatgcg tggtacgcgc
     4501 cgtctggtgc tgattatcgt cggttttctg atctttattt tcgccagcta ctccgcacag
     4561 cgttatctga ccgaagcggc aaatgggaca ttagctcttg atgttgtgct ggatatcgtt
     4621 ttctacaaag tactgattgc actggagatg ttgttacctg ttggtctgta tgtgtcagtt
     4681 ggcgtgacgc tagggcagat gtataccgac tcggaaatta ccgctatctc tgcggcgggg
     4741 ggcagtccgg gacgcttgta caaagccgtt ctttatctgg cgataccgct aagtattttt
     4801 gtcacccttc tgtcgatgta tggtcgtccg tgggcttatg cgcagattta tcaactggag
     4861 cagcagtcgc agtcggagct ggatgttcgc cagttgcggg caaagaaatt taacactaac
     4921 gataacggac gaatgatcct ttcgcagacg gttgatcagg ataataatcg cctgactgac
     4981 gcgctgattt atacttctac tgccaatcga acccgtattt tccgcgccgg ttcggttgat
     5041 gtggttgacc catcacccga gaaaccgacc gttatgttgc acaacggcac tgcctatctt
     5101 ctcgatcatc aggggcgtga cgacaacgaa cagatctacc gtaatctgca attacatctg
     5161 aatccgctgg atcaaagccc gaacgtcaaa cgcaaagcga aatcggtcac ggagctggcg
     5221 cgctccgcct ttcctgccga tcatgccgaa ctgcaatggc gacaaagccg tggcctgacc
     5281 gcattgttga tggcgctgct ggccatttca ttaagtcggg taaaaccgcg gcaagggcga
     5341 ttttcaacgt tattgccact gacgttgctg tttattgcca ttttttatgg cggcgacgtc
     5401 tgccgtacac tggtggctaa cggcgcgatt cccctcattc ctggtttgtg gttagtgccc
     5461 ggactcatgc taatgggcct gctgatactg gtcgcacgtg acttctcttt gctgcagaaa
     5521 ttttcccgat gaatgttttc agtcgctatt taatccgtca tctctttctc ggttttgccg
     5581 ccgctgcagg gctattgctg ccgcttttta ccaccttcaa cctgattaac gaactggatg
     5641 gtgtcagccc gggcggttat cgctggactc aggcggtgct ggtggtgcta atgaccttac
     5701 cgcgcacact ggtcgaactt tcgccgttta tcgcgttatt gggtggtatt gttggactgg
     5761 ggcagttatc gaaaaacagt gagcttaccg ccattcgcag cacggggttt tctatcttcc
     5821 gtattgcact ggtggcgctg gttgccggga tattgtggac tgtttcgtta ggcgcgatag
     5881 atgagtgggt ggcgtcgcca ttgcagcaac aggcgttgca aatcaaatcg accgccaccg
     5941 cgttggggga ggacgatgac attaccggca atatgctgtg ggccaggcgt ggtaatgaat
     6001 ttgtgacggt gaaatcgctg aacgagcagg gccagccggt gggcgtggag atatttcatt
     6061 atcgcgacga tctctcgctt gaatcctaca tttatgcacg cagtgccacc attgaagacg
     6121 acaaaacgtg ggtcctgcat ggtgtgaatc ataaaaaatg gctgaatggc aaagaaacgc
     6181 tggaaacatt agataatctt gcctggcaat cggccttcac cagtatgaat cttgaagagt
     6241 tatcaatgcc ggggaatact ttttctgtcc gtcagcttaa tcattacatc cattatttgc
     6301 aggaaaccgg gcaacccagc agcgaatacc accttgcact gtgggaaaaa ctggggcaac
     6361 ccatcctgac cctggcgatg attttgctgg ctgtgccgtt tacctttagt gccccgcgtt
     6421 cgcctgggat gggtagccgt ctcgctgtag gcgtcatcgt gggcttactc acctggatca
     6481 gctatcaaat catggttaat ctgggattgc tatttgcgtt aagcgcacct gttaccgcgc
     6541 tcggtttacc ggtagcgttt gtgttggtgg cgttgagcct ggtgtattgg tatgacagac
     6601 aacattaaac ccgccgtggc gcaaacacca cggcgggctc acattactgc ttcagagtag
     6661 caaatgcctg aatgatggca tcgatttgct cacgactgtg ggcagcattt acgctacagc
     6721 gcagcagcgt gatgcctgca ggcgcggcag gcggcaggat aagattcaca tacacgcccg
     6781 gcgagataag ctcgcgccag atgcgtaaac cttcctcttt cgaaccgatt attactggca
     6841 ctaccgggct aatatgtgaa cttaactcat aacccagttt ttgcagaccg tcatacagat
     6901 ggttggcgtt atccatcagc ttttgccgca gctctggttg tgtggcaata gttcttaacg
     6961 acgagcgcac ggtcgcaatg caggacggag aaggagaggc ggtaaaaata tacgggcggc
     7021 tgccgtagcg cagcacttcc atcgcttccg aaacgacagc aaaaccgcca atcgaggcca
     7081 gacttttact gaaagtacca acgataatat ccacatcatc ttcaacgccg acagcttcag
     7141 ccagtccgcg ccccgttgcg cctaacacgc caaacgaatg cgcttcatcg acaatcaaat
     7201 aaccaccgag acgacgtttg atatcaacaa tttccgccag cggcgcaacg tcaccgagca
     7261 tgctgtagat gccctcaacg atgataatcg cctctttcgc ccgttcaccg aggcgaacca
     7321 tgcgccgttc cagatcttta gcatcgttat ggcggaaacg aataatttcc gcgccaccga
     7381 gagaacagac gtcataaata ctggcgtggc tgtcggcatc cagtaacacc acggcgttat
     7441 ggtcggcaag tgcgctgatg acgcctaaat tagcggtata accggttgaa aaacgatagc
     7501 ggtagggcga ttgaaaaact cggcaatctc tttctctaac gccaggtgcg gcgcataact
     7561 gccgtttgcc atacgagaac cggtggtgcc ggtgccttgg gccgctaacg cggcctgacc
     7621 ttcgctgatg gcatcatgat taaaggttaa accgagataa ttattggtcc cggccagaat
     7681 cactttttta ttgccgatcc ggccttcggt ggcggaatac acttcatcaa tacaggtgcc
     7741 aaacggtgtt aacccgttgt cactaaattg cagacgctcg ccagcaaggc gcgcatattt
     7801 atcgtatagc cccattattt attctccagc catggaagta gagtttccat taattgcgca
     7861 ggggttttga catccagcag aatattaata ggaatggaaa tatcgaatct gtcttccagc
     7921 atcattaaga gatccatcac tttgatcgac tccaggccaa gatcgttgac gagatcgctg
     7981 tcgggcttaa tttccacacc gttttcgacc aaatcctgta aacaggaaag gatgtaatcc
     8041 attactattt cacgatttac catagaaacg ttaacacacc tttaaaacaa gacaatcaaa
     8101 atagcccgtt gcgtaatgcg tgttccaggc taatcccggg aaaccaattg atatcttcag
     8161 aaatacggtt attacttgcc gaccagtcgg cgtgggttaa ttcacgaatt ttcgagcggg
     8221 tcagcatagg ttctttacct gccaggcgac tcaacgcggt actgatatcc gcaaggcctg
     8281 tgagtaccgg cagaggaatg tcaaccattc gcacggaacc acaacgggct ttggcggcaa
     8341 gctgtcgtac gcgttgccag tcatagccac cggcgacgcc atcgcataat tcataggttt
     8401 gcgtctgtac agtttcggcg cttaaccact gacccactgc ttgcgcaaaa tcggtgacgt
     8461 gaagaaaaga gagttgtgta tctggcgcac caagtcgtgg cagcaggccg cgtagcatcc
     8521 agtcaaacag cggttttaac tctttatcgc cgggaccata cacggctgtc gggcgaaaaa
     8581 cgcccagcgt aatttcgtcc gccattgctg ccagtcgttg ctcggcgacg tatttggatt
     8641 ttgcatacca ggagagctcg ggatggcgcg ccgccagcga agagataaac agaaaacgtt
     8701 ggcaaaagcc gctctctttt gctgcttgca tcaggcgcag gctgccgtca acgttacagc
     8761 gggtgaaaat ctcttctttg tgcccgcgca cttgcccggc gcaatggacc actacgctgg
     8821 ctccggcaac cagctcgcta agtgaatgag tatcttccag cgaaccgcgc acccaggtga
     8881 gattatcatt gacgtgagcg cgggcagtac gcgtcaatgc gcgaacgtga aagccgcggg
     8941 cgagcaggtt atcgataata tatttaccga taaacccggt agcgcccgtc accgcgacag
     9001 tttgatttat gccagggatt cctgcacatt aagactggca gcataagcct tctgataacg
     9061 ttttttcgct tccgcacggg caggcttgcc ggaggacgtt cggggaatac tgtggggcgg
     9121 caacagatcg atagccgcgg tcacgccaaa ttcgctttgg atccgtgccg ccagcgcgtg
     9181 gataagctgc ccgcgacgtt cttcgtcgct gatccgacac tggatctgca aaatgatttt
     9241 ttcctgggcg gtaacaaaag caatcgcatc gccagaatga atttccggtt cctgttccgc
     9301 tatatattca atatcctgcg gccagatatt acggccacga ataataatca gatctttaat
     9361 gcgtccggtg acatacagat aaccgtccag cagataaccg aggtcgccgg tgtctaacca
     9421 gcccgtcgcg gcaatctcgt cttgcgaaac ctggtcgcca aagtaaccgc tcatcagact
     9481 gggaccggag atgcaaatat ggcctaccac acgttccgcg accggcatac ctgcttcatt
     9541 gcggatttca ataccatgtt ccggcaacgc tttgccgcag ttgacgaaag tcgatacggc
     9601 gcgtgtctct gcacccggcg cgacggcttt accctgatat tcgaggatgt cgcgatccac
     9661 ttcgttaacc acaaccccgg aggcttcatc agagaagctg acagccagcg cattttctgc
     9721 cagtccgtag cacggcatga aagttttatt gtcaaagtta acctgacgga aacattcagc
     9781 gaattgatgg agttgttctg cggagatcgg ctcagcacca ataccagcga cgcgccagca
     9841 ggaaagatcc agttcagcga gatctttttc attcacgcgg cgttggcaca attcatagcc
     9901 aaacggcggc gcaacggaaa cggtgccgcg atttttactg atcaatttaa gccattgcaa
     9961 aggacgcatg gcaaaatcct gagtgcgcaa ataatctact gaaagttgcg tggcgacggg
    10021 gggcaggaga aagccgacca gtcccatatc atggtagaaa ggctgccagg agacgcagcg
    10081 gtcgctaggg cgtaatttaa tgccatcgtg gcttatagca cgtagattag ccatcacttc
    10141 gcgatgggtg ataatgacgc cacggggaaa acgggtgctg ccggaggtgt actggaggta
    10201 ggcgatatcg ttcggaactg gacgctggag cgcaacatcg gcttccggta atgccttaaa
    10261 ccaagcgtgg cttaaaacat gtaattcggg gttgttatgc gtcgcggcat tgaccagtgg
    10321 caaccactca tcaccagtga taatggctgc gggctggcaa cttgccagta aaccctggag
    10381 tttggcgctc caggaatccc gctgaccgac gcccattgga atcgccaacg ggacggcgac
    10441 taagccagca tactgacagg caaaaaaagc ctctacaaac ccgctactcg tttcggcaat
    10501 cagcgccacg cgttcgcctt ttttcaggtt cagcgataac aaccgcttcg caccagcttc
    10561 ggcacgcgtt tttaatgtct gatattccag ttgatcttca agttggcaac gtctgtcata
    10621 aaaattcatt ccggctctac tcagagcggc gtagtccaaa gcatcaacca gcgttggaaa
    10681 atcggcatag cgcataggta gggaatgcgt aaagatttta ttagacatat acaccacgaa
    10741 aaaattggat atcaaaaata aaatgacaat a
//
LOCUS       DAZPCS010000127        10744 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZPCS010000127 DAZPCS010000000
VERSION     DAZPCS010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10744
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.127"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            106..936
                     /locus_tag="ACHUQ7_003076"
     CDS             106..936
                     /locus_tag="ACHUQ7_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFR1136379.1"
                     /translation="MSMPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1077..1850)
                     /gene="uxuR"
                     /locus_tag="ACHUQ7_003077"
     CDS             complement(1077..1850)
                     /gene="uxuR"
                     /locus_tag="ACHUQ7_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFR1136380.1"
                     /translation="MKSVTSAQRPYQEVGAMIHDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2065..3525)
                     /locus_tag="ACHUQ7_003078"
     CDS             complement(2065..3525)
                     /locus_tag="ACHUQ7_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFR1136381.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GTQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMGYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTSAYQQLCERGARECVAAL"
     gene            complement(3606..4790)
                     /gene="uxuA"
                     /locus_tag="ACHUQ7_003079"
     CDS             complement(3606..4790)
                     /gene="uxuA"
                     /locus_tag="ACHUQ7_003079"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFR1136382.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYKVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     IR"
     gene            5130..6473
                     /gene="gntP"
                     /locus_tag="ACHUQ7_003080"
     CDS             5130..6473
                     /gene="gntP"
                     /locus_tag="ACHUQ7_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HFR1136383.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(6724..7626)
                     /gene="fimH"
                     /locus_tag="ACHUQ7_003081"
     CDS             complement(6724..7626)
                     /gene="fimH"
                     /locus_tag="ACHUQ7_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFR1136384.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7646..8089)
                     /locus_tag="ACHUQ7_003082"
     CDS             complement(7646..8089)
                     /locus_tag="ACHUQ7_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1136385.1"
                     /translation="MQAADVTITVNGKVVAKPCTVSTTNATVDLGDLYSFSLMSAGAA
                     SAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQGTAQNIQLELQDDSGNTLNTG
                     ATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVISITYTYS"
     gene            complement(8162..8692)
                     /gene="fimF"
                     /locus_tag="ACHUQ7_003083"
     CDS             complement(8162..8692)
                     /gene="fimF"
                     /locus_tag="ACHUQ7_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFR1136386.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8702..>10744)
                     /gene="fimD"
                     /locus_tag="ACHUQ7_003084"
     CDS             complement(8702..>10744)
                     /gene="fimD"
                     /locus_tag="ACHUQ7_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFR1136387.1"
                     /translation="RIGGNSHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSGSKNK
                     WQHIITWIERDIIPLRSRLTLGDGYTQGDIFDGINFRGAQLASDDNMLPDSQRGFAPV
                     IHGIARGTAQVTIKQNGYGIYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGSTQIF
                     TVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEKPRFFQSTLLHGLPAGWTIYGGTQL
                     ADRYRAFNFGIGKNMEALGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNESGTNI
                     QLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQLT
                     VTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNAWQKGR
                     DQMLALNVNIPFSHWLRSDSKSQWRHASASYSMSHDLNGRMTNLAGVYGTLLEDNNLS
                     YSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANIGYSHSDDIKQLYYGVSGGVLAHAN
                     GVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTDWRGYAVLPYATEYRENRVALDTNT
                     LADNVDLDNAVANVVPTRGAIVRAEFKARVGIKLLMTLTHNNKPLPFGAMVTSESSQS
                     SGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCVANYQLPPESQQQLLTQLSAECR"
BASE COUNT         2575 a         2782 c         2644 g         2743 t
ORIGIN      
        1 actacaacag aaacatgcat gtcatttgtt tacttctctt tttactacag aaaaatttat
       61 ctatttttct aaacatcagt taaatactca taaaaggtaa ctattatgtc tatgccatta
      121 agcaatgccg ctaataacca attcaataca aataatcact tccttcatca tccaaaagtc
      181 gacagtgagt taacacgtcg atatgaatat gcacgcctgg atacggaaaa tatctatctt
      241 ctgcctcttg ctcgtggcaa taaccataat tacgatggaa aaagcgttgt cgaaattaga
      301 aaacttaata ttggcaaaca gtcctggcca tttaattatg tgacagatac atgcagagaa
      361 ttcaatggta ttacaacaac aggacgtatg ctctatcgaa acttgaaaat cacctcagct
      421 ttagacgaaa tatatggtgg tatatgtaaa aaggcgcatg ctgctacaga gttagcagaa
      481 ggtttacggc taaatttatt tatgaagagc cctttcgatc ctgttgagga ctatacagtt
      541 catgaaataa cattaggccc tggttgcaat gttcctggtt atgccggaac cacgatagga
      601 tatatttcaa cccttccagc ttctcaggca aaaagatgga ccaatgagca gccaagaatt
      661 gatatctata ttgatcagat aataactgtc tcaggggtag caaattcttc cggattcgcc
      721 cttgccgcat tacttaatgc taatattgag atgggaaatg acccaatcat tggtatagaa
      781 gcctatcctg gtaccgcgga gatccattca aaaatgggat ataacgttat ccccggtgat
      841 gaagatgcgc cactcaaacg catgactctg caaccgtcat ctcttccaga actgtttgaa
      901 ttaaaaaatg gtgaatggaa ttatattggt aaatagccac tgagaagaat gagtactaat
      961 attcaggtta tacagatatt taaacaaaac cctgcaattt acaaagtaat actcattgcg
     1021 acttcttaat ttaagaaagc gcaaggaacg tttacccttg cgcttattat aataagtcag
     1081 gcgtcgactt tatccagcgg ccatgaatca aagagatagc cgtcaaaata aatatcgtca
     1141 acgttcgaga attccagcag acgttgctta acgttttcca gatgctgcca cattgccagc
     1201 ttcgccgctc gggcatcttt tttgattaac gcggcgagga tctgtttgtg atcgcccaac
     1261 cactctttgc gatacaggct gtcatccaga tggctgtgca actggatcca cattggattg
     1321 ttttcccgcc actgccagga ctgacggaat aactccacca gcatgctgtt atgcgttgct
     1381 tcagcaatgg cgagatggaa ctgcatgtca ccgctttcgc tgctacccgg cgcgctggaa
     1441 gccagttcac gctcttccag ttgcaatgcc tgacgcattt tgacgatatc ttcgcgggta
     1501 gcctgcagtg cggcaaactc ggcgatgtta ctttccagta actgacgcgc ctgtaacagc
     1561 tcaaaaggac ccgcatcatt gcagacgttg gcatccggac tgtctgtgtt gtggctgcct
     1621 gagctatcaa gcacatagat accggcaccc cggcgcactt ccaccagccc tttgatctcc
     1681 agcatgatca gcgcttcacg taccaccgtc cgcgtgacat caagcatttc tgcaatttca
     1741 cgctccggcg gcagccgttc gccaggattg tacggcgtct ttatgatcag atcgtggatc
     1801 atcgccccga cttcctggta aggtctttgc gcagaggtga cagatttcat ggtgttgtgt
     1861 ccgttaggtg cggttaatca aatttttgcc ggatgcggcg tgaacgcctt atccggccta
     1921 caacgggcaa ttttacggcc cccgttaatc tcaaactata ccattagcac tggaagttgg
     1981 cacgtacagg tgggcaaaaa aacgaaaact ggtcaaccaa atatcagttt gattgaccag
     2041 taagtctgta gggtaatcag ttagttacag cgcagccaca cactcgcgcg caccgcgttc
     2101 gcacagctgc tgatatgccg acgtcaccgc gccaacaaag tcggcattct gcggcagatc
     2161 atcggcaaaa ataccgctca ggcccagcag cgctttcacg cggtctgcgc cctgatactg
     2221 cgcgttaatc ttctggaact cggccagcat cgggtcgacc acgtcaatgg cattaccctg
     2281 ctcatccacg ccctgggtgt aacccatcca gccagccacg cccagcgcca ggtgacgcca
     2341 gctgccgccg ttttgcaggt gcagacgcac cgggtccagt agacgctgcg gtaacttctg
     2401 gctgccgtcc atcgcaatct gccaggtacg gtgacgcaga gacgggttgc tgaaacgctc
     2461 gatcagcagc gtcgcatagg cgttcaggtc cgtaccttcc ggcatcgaca gcgttggcgc
     2521 ttgttcctgc atcatcaggg caaaggccgc tttgcgataa tccgggttag tcatggtgtc
     2581 ggcaatggtt tcatagccgc cgaggtaacc gaggtacgcc aggaaagagt ggctgccgtt
     2641 cagcatacgc agcttcatca tttcgaacgg cacaacgtct gcaacgaact gtgtgcccac
     2701 tttatcccag tccgggcgac cattaacgaa gttatcttca atcacccact gacggaacgg
     2761 ttcgcaggca atggcgcacg ggtcgtaaac acccagctgg tcagcaattt cctgtaaggt
     2821 ttctggcgtc gccgccggaa cgatgcggtc aaccatggtg cacgggaagg tgacgttctc
     2881 ttcaatccat gccgctagct gcgggtcacg cgcctgagcc agccccagta ccgcgacctt
     2941 cgccacatgg ccgttttcac gcacgttatc gcaggacatc accgtaaatg ctttcagccc
     3001 tttttcacga cgcagacgca aggcttcgac gatgtaacca atcgcggact tcggcgcagt
     3061 cgggttttcc agatcgtgct tgatcagcgg gttattgaga tccagttgac cgcttgccgc
     3121 atcagcgcag tagccttttt ccgtgaccgt tagagagaca atcgccgttt gcggacgcgc
     3181 catcgcgttg agaatacctt cgcagccgtc gatttccgga tgcagcgctt ctttcatcga
     3241 accgataatt ttcagctcgg tgctttctgc gcctttttcc gctacggtgt acagcagttg
     3301 ctgttttttc aggttttcga tcagcatgcg gtcgttgcct ggcatcaggt taacttcgca
     3361 gatgccccag tcgctgtcgg tgctttccag cagatgatgg gtatacagcg cctggtgcgc
     3421 gcggtgaaac gccccgcaac cgagatgcac aatgcgtgat tccagacgag aatgatccca
     3481 tgacgggcgg gcaaccggca gattgctgtc aacaatagta gtcatcgtaa actccagaat
     3541 ccgtcttcag agagtgacgg caaggaagaa ggaaccggca cggcgcgcag ccatgccggt
     3601 gggtattaac ggataaagaa agcgcgctgg atcgccagtt cgacaccgcg aacttcggcc
     3661 aggcctttca gacgaccaat tgcggagtaa ccagggttgg ttttcttctt caggtcgtcc
     3721 agcatctgat gaccgtggtc cggacgcatc gggatcaggt cttctttgcc ttccgcttta
     3781 cgacggtgtt cttcttcaac aatcgctttc accactttgt acatatcaac gtcaccgttc
     3841 aggtgcgccg cttcgtggaa ggttttcggg ttatcttcac gcatggtgga gcgcagatgg
     3901 gtgaagtaaa tacgcggacc gaactgcttg atcatatcaa ccagatcgtt gtcagcacgc
     3961 acgccgtagg aaccggtgca catggtgaaa ccgttcgcca tgctgtttac ggtatcaacc
     4021 atccactgca tatcttcaat ggtggaaaca atgcgcggca agccgaggat cgggcgcggc
     4081 ggatcgtccg ggtgaaccgc catacgcacg ccaacttctt cagcaactgg aataatcgct
     4141 ttcaggaaga cggcaaagtt ttcgcgcagt ttggctttgt cgatatcttt gtacagctcc
     4201 aggtgcttac ggaactggtc gagggtatac ccttcttccg cgcccggcag accggcaatg
     4261 atgttacgcg tcagacgcgc tttgtcttcg tcgctcatag tggcgaagcg ttcagctgcc
     4321 tgagcaattt cttcttcggt gtaatccgct tccgcgcctg gacgtttcag gatatgcatt
     4381 tcgaatgcag cgaattcgat ctggtcgaag cgcagagctt tggagccgtc tggcagcacg
     4441 tattcgaggt cagtacgggt ccagtcgagc accggcatga agttgtagca cacggtgcga
     4501 atgccacact gcgccaggtt gcgcagagtc tgctgatagt tagcgatcca ctgctcatag
     4561 ttgccagtgt gggttttgat atcttcgtga attggtacgc tttctacgac agaccacacc
     4621 aggcctgcgt cttcaacgat cgccttgcgt ttgaggatct cttctacgga ccatacttcg
     4681 ccgttcggga tatggtgcag cgcggtaacc acgccagttg cgcccgcctg acggacatca
     4741 gctaaagaaa ccggatcgtt tgggccgtac cagcgccagg tctgttccat gatttttcct
     4801 ctttcacata gtgggttcca cagcaaagag acgcggaacc ttggagttgt ttaggtatcc
     4861 ggatgaacaa ccagaccgca gccgtggcgt tgcatcggat gagatggcgt ataagttcta
     4921 ccttttggta agacatgtca accagaattc tgaaattggt taaccgcgtc gcaagaattt
     4981 cactctcatg gttgttgctg caaattgcag caaagggtaa tcgatggtta ttgatcagag
     5041 gatgtgacat tcatcgcaac aatggttgac caatttacat aacatgtcgc ccaaaataac
     5101 aacggttcaa ccaagtaagg taaaacgtta tgcatgtgct taacattctc tgggtggtat
     5161 tcggcattgg cctgatgctg atactgaatt tgaagttcaa aatcaattca atggtggctt
     5221 tgttggtggc ggcgctgtcc gtcgggatgc tggcaggcat ggatttgatg tcgctgctgc
     5281 acaccatgaa agcgggcttc ggcaacacgc tgggagaact ggctatcatc gtggtgttcg
     5341 gtgcggtcat cggtaaattg atggtcgact ccggcgcggc tcaccagata gcacatacgc
     5401 tgctggcgcg tctcggtctg cgctatgtac agctgtcggt gattatcatc ggccttattt
     5461 tcggtctggc gatgttctat gaagtggcct ttatcatgtt agcgccgctg gttattgtta
     5521 ttgccgccga agctaaaatt ccgttcctga aactggcgat cccggcagta gcagctgcca
     5581 ctaccgcaca ttcactgttc ccaccgcagc cgggtccggt ggcgctggtg aatgcttatg
     5641 gcgcggatat ggggatggtt tatatctatg gcgtactggt gacgatccca agtgtaatct
     5701 gcgcaggtct gatcctgccg aagttcctcg gcaatcttga gcgcccaacg ccatcattcc
     5761 tgaaagcaga tcaaccggta gatatgaata atctgccctc tttcggcgtt tcgattctgg
     5821 tgccgctgat cccggcgatc attatgatct ctaccaccat cgccaatatc tggctggtaa
     5881 aagatacccc tgcctgggaa gtggttaact ttattggttc ctcgccgatt gcgatgttta
     5941 ttgcgatggt ggttgcattc gtactctttg gcaccgcgcg tggtcatgac atgcagtggg
     6001 tgatgaacgc ttttgaaagc gcggtgaaga gtattgcaat ggtgattctg atcatcggtg
     6061 cgggtggcgt gctgaagcag accatcatcg acaccggcat tggcgacacc atcggcatgt
     6121 tgatgtccca cggcaatatc tcgccctaca ttatggcatg gctgatcact gtgctaattc
     6181 gtctggcgac gggtcagggt gtcgtttcgg cgatgaccgc cgccgggatt atcagtgctg
     6241 caatccttga tccagcaacc ggtcagctgg ttggcgtgaa tccggcgctg ctggtactgg
     6301 cgacggctgc gggttccaac accctcaccc acattaatga tgcatctttc tggttgttca
     6361 aaggttactt tgacctgtca gtaaaagaca cgttgaaaac ctggggtctg ctggagctgg
     6421 tcaactccgt ggttgggctg attattgtgc tgattattag catggtagcg taaaagaaca
     6481 ccagagcctg ccaatggcag gctcagactg atgactaaag tctaaaaatg tgcccggaca
     6541 gtcccctcgc ccctccgggg agagggttag ggtgagggga acagaccagt aaagtgcgct
     6601 ttgtttatgc cagatgcgac gctgacgcgt cttatctggc ctacaaaggg ctaacgtgca
     6661 ggttttgtag gtctgatagc gtagcgcctc aggtaccagc attagcaatg cgctgtaatt
     6721 tttttattga taaacaaaag tcacgccaat aatcgattgc acattccctg cagtcacctg
     6781 ccctccggta cgtgcgtaat ttgccgttaa tcccagactt accgccgaag ttcctactgc
     6841 tcctaacgat accgtgttat tcgctggaat aatcgtaccg ttgcgcgtca actgtacgcc
     6901 gacgccctgc gctggtgaaa acgacgcggt attggtgaaa atcgagttgc ccgcatctgc
     6961 ggttgtgccg gagaggtaat accccaggtt ttggcttttc gcacaataaa cggtaagagg
     7021 aattggcact gaaccagggt agtccggcag agtaacggtg acatcacgag cagaaacatc
     7081 acaaccgcca gtgggcacca ccacatcatt attggcgtaa atattccaca caaactgaaa
     7141 atcatcgctg ttatagttgt tggtctgtcg caaaataagc acggcaatta atgaaccagc
     7201 tttaatagcc accccgcccg cactgctcac aggcgtcaaa taaagcgcca ccggccacgg
     7261 cttatccgtt ctcgaattat aaacaacccg cggcgtttcg ctggtggtcg gaaatggata
     7321 gctactgcca ctatatttta cggtcccgga aaaattagat aacacgccgc cgtaagccga
     7381 gcctcgttgc agtgtgacat agtctgtaat ggtttccgga taatcgttat ggcaaaagat
     7441 ttgcgtcgaa agatctacga ccaggttttg ccccacattc acgacaggcg caaggtttac
     7501 ataaacatta gcgctgccac cgccaatagg gatagcggta ccattggcgg ttttacaggc
     7561 gaatgaccag gcatttaccg accagcccat cagcagtaca gcaaacaggg taatagctcg
     7621 tttcattaca attatctctt cgggttcagc tgtaggtata ggtgatgcta atcactgcct
     7681 gaatggttcc ctgagtggct ccgccattta ccgtcaatgc tctgacctgt aacgggaagt
     7741 gcgctgattg tgaggaatca tccacctgaa ctgttttggt tgcgccagta ttcaatgtgt
     7801 tgccactgtc atcctgtagc tctaactgga tgttttgcgc ggtcccctgg tttttataat
     7861 atccggtgct gtcggctgcc ccgctgaagc tggcagtgac cctcgacgtt cccaccggac
     7921 aattagtcaa ctcaagcgca acatcatgcc aggccgatgc cgccccggca gacataagac
     7981 tgaaagaata aagatcgccg agatctaccg tggcattggt ggtggaaacc gtacacggtt
     8041 tggcgacgac cttaccgttc accgtgatgg tgacatcggc tgcctgtatc gtcgcacttg
     8101 cgagcgccaa cattgccgcc aatacatacc cacgtttgca ccatttcatg agcatctcca
     8161 gttactgata ttcaagagtg aaggtagccg tagcattgat atgccccgca gtgacaggca
     8221 cctgtgtcgc cattagccgg gcgtaaaaat tcagcgtatt tggtttaccc ggcgtcaggg
     8281 tcgtccacga aatcgcagac gatggagcat taaggggtat ttgattttgc tgctcattca
     8341 gaagctgtat tcccagtccc gcagccgctg acaccgtatt ttcaagtgca agcaggttgg
     8401 cattgtggct atctgcaacg ccggtaaacc caacttttac ggcagaaacg gcattaccac
     8461 agggtgacag caaaatacga aatggaacga caggagtcgt cgcgccaatg ttgttaaatt
     8521 gcttcgccgc gttttccatc agatcaacag taaaattggt tgattcagcg gccacactac
     8581 agccgttatc tctgacatag ccgcggatag taatcgtgct atccgcagcc aaaacgcgac
     8641 ttaccgccag ccacaaaaaa gcgcacagaa gataaaaagg tttgtttctc atcacgcccc
     8701 cttaacgaca ttcagctgat agctgggtta ataactgctg ctgactctct ggtggcagtt
     8761 gataattggc gacacagtga gcattttcct cttctcccca tttcacctga acttttcccg
     8821 ctaaaggcat tccgctgagg taaacctgac cattatccgc aacaatgccg ctactctggc
     8881 tactctctga tgtcaccatc gccccaaacg gcagcggctt attattgtga gttagcgtca
     8941 tgagcagttt tatcccaacg cgcgctttaa actctgctcg cacgatcgcc ccacgagtgg
     9001 gaacaacgtt agcgaccgcg ttatctaaat cgacgttatc agccaaggta ttggtatcca
     9061 gcgccactct attttcccga tattcagtgg cataaggcag cacggcataa ccacgccagt
     9121 cggtacgcac ccccgtctgg ttttcgactt ttgcatcttt tgcgccaggc gctttaacaa
     9181 gcaccaccgt atcgtttaac ggctgcccca gcgttacgcc attggcatga gccaataccc
     9241 caccgctgac tccgtaatag agctgcttaa tatcatcgct atggctgtaa ccgatattgg
     9301 cattgccgta accaccgcga taattcagcg tggcgtagcc tgtacttccg ctattaccat
     9361 cgcctccccc ggcatagccg gtttgcacgc tataactgag gttgttgtct tccagcaacg
     9421 taccgtatac accagccaga ttggtcatcc gaccgttgag atcgtgtgac atgctgtagc
     9481 tggcactggc atgtcgccac tgagatttac tgtcagaacg cagccagtgg ctgaaaggaa
     9541 tattgacgtt aagcgctaac atctgatccc gtcctttttg ccaggcgttt ttcgtcaggc
     9601 tatagctgag cgtccagttg atatcttcga acgcagtatt taatccagcc tggaattgct
     9661 catcgacatt actcgttccc caataagttt gatggctacc actcaaatac agtgttgatg
     9721 tgcgcccgag ttgctgagta acggtgagct gtaatttccc gcgtttgtta taagcgaggt
     9781 tgtaatagtc ggtgaatttc ggcttaacct gaataactcc atcctgtgtt tcgatgttgt
     9841 agccattcat tcgactgtat gttgtatcag cgaaattaaa atatccgctg gtcgaataac
     9901 ggtaacccac taactgaata ttcgtgcctg attcattgag cgatttgtta tagagaaaac
     9961 gcaccgattg tccgtcatgc tgactgtcat cgggaagtgt ggaattagcc tgcgtcatat
    10021 ccacagacag agcgcccagt gcctccatgt ttttcccgat accgaaatta aaagcacgat
    10081 aacgatccgc cagttgcgtt ccaccatata ttgtccagcc agccggaagg ccgtggagta
    10141 atgtactctg gaaaaagcgg ggtttttcct gctgcgcatt tccactacgg tattctcctg
    10201 ccgtaatgga ataacgagta tgcccttcac gttgcaaaag cgggactgac gaatagggta
    10261 cggtaaaaat ctgcgtgctg ccgtcagcct ctttgatcgt tacctgcaag tcaccactat
    10321 tacctgcggc atagatatcg ttgatggtaa aaggccccgg tggcaccgta ctattataaa
    10381 tgccatatcc attttgttta atagtgacct gtgcagtacc acgagcaata ccgtggatca
    10441 ccggggcaaa tcctctttgg ctatcgggta acatattgtc atctgaggcc aattgtgcgc
    10501 cgcgaaagtt aataccatcg aaaatatcgc cctgggtata accatcaccc aacgtcagcc
    10561 gggaacgtaa aggaattatg tctcgctcaa tccaggtaat gatatgctgc catttatttt
    10621 tgctacctga tgaactgtcg ctactgttat aactccaggt ggtattgtcg cgtaaacgcc
    10681 acgcaccaat atttaaccca ctctgtaggt ttaaatatgc ataatggctg ttacccccaa
    10741 tccg
//
LOCUS       DAZPCS010000128        10604 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZPCS010000128 DAZPCS010000000
VERSION     DAZPCS010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10604
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.128"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..1368
                     /locus_tag="ACHUQ7_003085"
     CDS             <1..1368
                     /locus_tag="ACHUQ7_003085"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFR1136388.1"
                     /translation="ELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQIINDELYLDGN
                     ARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVNMVADLWHAPAP
                     KNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCGPVQICWHKFAR
                     YWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYEFPQPLHDALDK
                     FQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWV
                     IWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNA
                     SYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRG
                     WQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQN
                     SFKHT"
     gene            1524..3059
                     /gene="gadC"
                     /locus_tag="ACHUQ7_003086"
     CDS             1524..3059
                     /gene="gadC"
                     /locus_tag="ACHUQ7_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFR1136389.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            3190..4509
                     /locus_tag="ACHUQ7_003087"
     CDS             3190..4509
                     /locus_tag="ACHUQ7_003087"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFR1136390.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            4886..5956
                     /gene="dosC"
                     /locus_tag="ACHUQ7_003088"
                     /pseudo
     CDS             4886..5956
                     /gene="dosC"
                     /locus_tag="ACHUQ7_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
     gene            5956..6573
                     /locus_tag="ACHUQ7_003089"
                     /pseudo
     CDS             5956..6573
                     /locus_tag="ACHUQ7_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            6831..7163
                     /gene="ddpX"
                     /locus_tag="ACHUQ7_003090"
                     /pseudo
     CDS             6831..7163
                     /gene="ddpX"
                     /locus_tag="ACHUQ7_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            7177..8073
                     /gene="ddpC"
                     /locus_tag="ACHUQ7_003091"
     CDS             7177..8073
                     /gene="ddpC"
                     /locus_tag="ACHUQ7_003091"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFR1136391.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLMR
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTSV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            8070..9044
                     /locus_tag="ACHUQ7_003092"
     CDS             8070..9044
                     /locus_tag="ACHUQ7_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR1136392.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIAPAFSCEPQLIIAGEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCALTACGDNNQHCTCWYPQQEVISV"
     gene            9037..9963
                     /locus_tag="ACHUQ7_003093"
                     /pseudo
     CDS             9037..9963
                     /locus_tag="ACHUQ7_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
     gene            complement(10019..10450)
                     /gene="osmC"
                     /locus_tag="ACHUQ7_003094"
     CDS             complement(10019..10450)
                     /gene="osmC"
                     /locus_tag="ACHUQ7_003094"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFR1136393.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         2568 a         2627 c         2686 g         2723 t
ORIGIN      
        1 gaactactcg attcacgttt tggtgcgaag tctatttcca ctatcgcaga atcaaaacgt
       61 tttccgctgc acgaaatgcg cgacgatgtc gcatttcaga ttatcaatga tgaattatat
      121 cttgatggca acgctcgtca gaacctggcc actttctgcc agacctggga cgacgaaaac
      181 gtccacaagt tgatggattt atccattaac aaaaactgga tcgacaaaga agaatatccg
      241 caatccgcag ccatcgacct gcgttgcgta aacatggttg ccgatctgtg gcatgcgcct
      301 gcgccgaaaa atggtcaggc cgttggcacc aacaccattg gttcttccga ggcctgtatg
      361 ctcggcggga tggcgatgaa atggcgttgg cgcaagcgta tggaagctgc aggcaaacca
      421 acggataaac caaacctggt gtgcggtccg gtgcaaatct gctggcataa attcgcccgc
      481 tactgggatg tggagctgcg tgagatccct atgcgccccg gtcagttgtt tatggacccg
      541 aaacgcatga ttgaagcctg cgacgaaaat accatcggcg tggtgccgac tttcggcgtg
      601 acctacaccg gtaactatga gttcccgcaa ccgctgcacg atgcactgga taaattccag
      661 gccgacaccg gtatcgacat cgacatgcac atcgacgccg ccagcggtgg ctttctggca
      721 ccgttcgtcg ccccggatat cgtctgggac ttccgcctgc cgcgtgtgaa atcgatcagt
      781 gcttcaggcc ataaattcgg tctggctccg ctgggctgcg gctgggttat ctggcgtgac
      841 gaagaagcgc tgccgcagga actggtgttc aacgttgact acctcggtgg tcagattggt
      901 acttttgcca tcaacttctc ccgcccggcg ggtcaggtga ttgcacagta ctatgaattc
      961 ctgcgccttg gtcgtgaagg ctataccaaa gtacagaatg cttcctacca ggtcgctgcc
     1021 tatctggcgg atgagatcgc caaactgggg ccgtatgagt tcatctgtac cggtcgcccg
     1081 gacgaaggca tcccggcggt ttgcttcaaa ctgaaagatg gtgaagatcc gggatacacc
     1141 ctgtacgacc tctctgaacg tctgcgtctg cgcggctggc aggttccggc cttcactctc
     1201 ggcggtgaag ccaccgacat cgtggtgatg cgcattatgt gtcgtcgcgg cttcgaaatg
     1261 gactttgctg aactgttgct ggaagactac aaagcctccc tgaaatatct cagcgatcac
     1321 ccgaaactgc agggtattgc ccagcagaac agctttaaac atacctgata acgtttaacg
     1381 gtaacggtgt cccgaaacga acccgtttcg ggataatttc caaagtctgt tcactggcat
     1441 tagcaacgga aaatattgtt ctgaatacgc ttcagaacaa aacaggtgcg gttccgacag
     1501 gaataccgtt ttagggggat aatatggcta catcagtaca gacaggtaaa gctaagcagc
     1561 tcacattact cggattcttt gccataacgg catcgatggt aatggctgtt tatgaatacc
     1621 ctaccttcgc aacatcgggc ttttcattag tcttcttcct gctattaggc gggattttat
     1681 ggtttattcc cgtgggactt tgtgctgcgg aaatggccac cgtcgacggc tgggaagaag
     1741 gtggtgtctt cgcctgggta tcaaatactc tgggtccgag atggggattt gcagcgatct
     1801 catttggcta tctgcaaatc gccattggtt ttattccgat gctctatttc gtgttagggg
     1861 cactctccta catcctgaaa tggccagcgc tgaatgaaga ccccattacc aaaactattg
     1921 cagcactcat cattctttgg gcgctggcat taacgcagtt tggtggcacg aaatacacgg
     1981 cgcgaattgc taaagttggc ttcttcgccg gtatcctgtt acctgcattt attttgatcg
     2041 cattagcggc tatttacctg cactccggtg cccccgttgc tatcgaaatg gattcgaaga
     2101 ccttcttccc tgacttctct aaagtgggca cactggttgt atttgttgcc ttcattttga
     2161 gttatatggg cgtagaagct tccgcaactc acgtcaatga aatgagcaac cccgggcgcg
     2221 actatccact ggctatgtta ctgctgatgg tggcggcaat ctgcttaagc tctgttggcg
     2281 gtttgtctat tgcgatggtc attccgggta atgaaatcaa cctctccgca ggggtaatgc
     2341 aaacctttac cgttctgatg tcccatgtgg caccggaaat tgagtggacg gttcgcgtga
     2401 tctccgcact gctgttgctg ggtgttctgg cggaaatcgc ctcctggatt gttggtcctt
     2461 ctcgcgggat gtatgtcaca gcgcagaaaa acctgctgcc agcggcattc gctaaaatga
     2521 acaaaaatgg cgtaccggta acgctggtca tttcgcagct ggtgattact tctatcgcgt
     2581 tgatcatcct caccaatacc ggtggcggta acaacatgtc cttcctgatc gcactggcgc
     2641 tgacggtggt gatttatctg tgtgcttatt tcatgctgtt tattggctac attgtgttgg
     2701 ttcttaaaca tcctgactta aaacgcacat ttaatatccc tggtggtaaa ggggtgaaac
     2761 tggtcgtggc aattgtcggt ctgctgactt caattatggc gtttattgtt tccttcctgc
     2821 cgccggataa catccagggt gattctaccg atatgtatgt tgaattactg gttgttagtt
     2881 tcctggtggt acttgccctg ccctttattc tctatgctgt tcatgatcgt aaaggcaaag
     2941 cgaataccgg cgtcactctg gagccaatca acagtcagaa cgcaccaaaa ggtcacttct
     3001 tcctgcaccc gcgtgcacgt tcaccacact atattgtgat gaatgacaag aaacactaac
     3061 catgtctgaa tggttataag acaagggagc gataattcat cgctcccttt ttcgtgcttg
     3121 ctgtcgtatt gacttaaccg ggataagtac gagaatgagc gcacatctgt ttaccggaaa
     3181 ccagcacata tggatatctg ctcccgaaac aagaaattaa cgattagaag accagcgata
     3241 ctggttgcat tggcactttt actgtgtagt tgtaaaagca cgcctccaga gtccatggtg
     3301 acaccaccag caggttcaaa gccaccagcc acgacgcaac aatcgtcaca accgatgcgt
     3361 ggcatctggc tggccacggt ttctcgcctc gactggccac cggtttcctc ggttaacatt
     3421 agtaatccca ccagccgagc ccgtgtacaa caacaggcga tgatcgacaa actggatcat
     3481 ctccaacgtc tcggcataaa cacggtcttt ttccaggtca agccggacgg taccgccctg
     3541 tggccatcga aaattttgcc gtggtccgat cttatgaccg gtaagattgg tgaaaatccg
     3601 ggttacgatc cgctgcaatt catgctcgac gaagcccaca agcgtgggat gaaagtacac
     3661 gcctggttta acccctatcg cgtatcggtt aatacgaagc ccggtactat cagggaactg
     3721 aatagcactc tgtctcaaca accggcgagc gtctatgtgc aacaccgtga ctggatcaga
     3781 acgtctggcg atcgctttgt cctcgacccg ggcatccctg aggttcagga ctggatcaca
     3841 tcaatagtcg ctgaagtggt ttcccgctat ccggtagatg gcgtgcagtt tgacgactat
     3901 ttctatactg aatcaccggg ttcacggcta aatgataacg aaacgtaccg taaatacgga
     3961 ggcgcatttg cgtcaaaagc agactggcgg cgcaacaata ctcagcagtt aattgcaaag
     4021 gtatcacaca ccattaaaag cattaagccg ggagtcgaat ttggcgttag cccggcaggc
     4081 gtgtggcgta accgatcaca cgatccgctc ggttccgata cccgaggcgc ggcagcctat
     4141 gacgaatcct acgcagacac ccgtcgatgg gtggaacaag gattgctgga ttacattgct
     4201 ccccaaattt actggccgtt ctcacggagt gccgcgcgtt atgacgtgtt ggcaaaatgg
     4261 tgggcggatg tcgttaaacc aaccaggacc cgcctgtata tcggtatcgc cttctataaa
     4321 gtgggtgaac cttcaaagat agagccagac tggatgatta acggcggcgt accggaactg
     4381 aaaaagcagc tcgatcttaa cgatgcggta ccagaaatta gcggcaccat cttgttccgt
     4441 gaggactatc tgaataaacc gcagactcaa caagcggtca gctatctgca aagtcgttgg
     4501 ggcagttaaa ctaccatgct tactttgtaa caagccggtg ctttacccac cggcttgtta
     4561 caccttgtga aatatcccac tagtaatcat gcttacataa gtcaaattaa ccacgcaatt
     4621 aaccacactt agttaaacag ctataaatcc ctaataaaaa tgtatgaata tgaaaaacca
     4681 tacgatattc gctgtcaccg aaaaataatt acgatgggct atactcttct gaagaacagt
     4741 agcgtagatt gaaactgtct taagtcgctt atttcttttc agtatatctt catatttcag
     4801 gagaatatgg tgcagttagt cgatctcgca cgttgcgttt cgttttgatt ccgtctgatt
     4861 tcccagcctt ataagggtgt gaattatgga gatgtatttt aaaagaatga aagatgagtg
     4921 gaccggactt gtcgaacagg cagatcctct cattcgtgct aaagccgcag aaattgccct
     4981 tgcgcatgct cattatctga gtatcgagtt ttatcgaatt gtccgcatcg acccgcatgc
     5041 cgaagaattc ttgagtaatg aacaagttga gcggcagttg aaaagtgcga tggaacgctg
     5101 gattattaac gtgctttctg cccaggttga cgatgtcgaa aggctaatac aaatccagca
     5161 taccgtcgcg gaagtgcatg cccgcatagg aattccggta gaaattgtcg agatggggtt
     5221 tcgggtgctg aaaaaaatcc tctatccggt aatcttctct tcggattatt ccgccgcaga
     5281 aaaacttcag gtctaccatt tctcgattaa cagtattgat atcgcgatgg aagtgatgac
     5341 ccgcgcgttt acctttagtg acagtagtgc ctcaaaggaa gatgaaaact atcgtatctt
     5401 ctcgttactg gaaaacgccg aagaagaaaa agaacggcaa atagcctcaa tactttcatg
     5461 ggaaatagat attatctata aagtcctgct ggattctgat ttaggcagca gtttgccttt
     5521 aagccaggct gattttggcc tgtggtttaa ccataaaggt cgacattatt ttagcggcat
     5581 tgccgaagtg ggccatatct cccgtctgat tcaggatttc gacggtattt tcaatcaaac
     5641 catgcgtaac accaggattt tgaataacag aagtttgcgg gtgaaatttc tattacagat
     5701 aagaaatacc gtatcgcaga ttattacctt gctgcgtgaa ttgtttgaag aagtatcgcg
     5761 ccacgaagtc ggtatggatg tactgacgaa attacttaac cgccgtttcc tgccgactat
     5821 cttcaaacgc gaaattgccc atgccaaccg gaccggtaca ccgctgtcag tgctgattat
     5881 tgacgttgat aaattcaaag agatcaacga tacgtggggc cataacactg gcgatgaaat
     5941 tctgcgtaaa gtctcattcc tctcgcagaa gagattggtg aaatcgaaaa tattgggcgc
     6001 tgggtcatcg cggaagcttg ccgtcagtta gcagaatggc gtagccagaa tattcatatc
     6061 ccggcgttat ccgtgaactt gtcggcgctg cactttcgca gtaatcagct gcctaatcag
     6121 gtgtctgatg caatgcaagc ctggggtatt gacggccacc agctgacggt ggaaatcacg
     6181 gaaagcatga tgatggaaca cgataccgaa atctttaagc gcattcagat cctgcgtgat
     6241 atgggcgtgg gtttatcggt agatgatttt ggcacgggct tttccggatt atcccgctta
     6301 gtcagtcttc cggtaacgga aatcaaaatt gacaaaagct ttgtcgatcg ttgtctgacg
     6361 gaaaaacgca tcctggcctt acttgaagcc attaccagca ttgggcaaag cctcaattta
     6421 accgtcgtgg cggaaggggt cgaaaccaaa gagcaatttg agatgctacg taagatccac
     6481 tgtcgcgtta ttcagggata tttcttttcc cgcccccttc ccgccgaaga aattccaggc
     6541 tggatgagca gcgtgttacc gctgaaaatc tgacaaattc ctctcgcccg cactcgcggg
     6601 ttttccttta aggtgacact gtcacttaaa tactgtgatt tcagctacga ttccgggaga
     6661 ttccttcctt aactttccct cttgcaccaa aactgcacta cgctgcacaa atgttgatca
     6721 aaagttaaaa aatataactt taagccagca ttctggcgct tatcccggca tggcatgaga
     6781 tctgcataag cggaaagcgc agcaattttt gtcttataca ggaaccgttt atgtcggata
     6841 ccaccgaact ggttgattta gccgtgatct tccctgatct ggagatcgaa ttgaaatatg
     6901 cctgcgttga taacatcaca ggtaaagcta tttatcagca agcgcgttgt ctgctacata
     6961 aagatgcgat taccgcgctg gcgaaaagta tcagtatcgc ccagctgtca ggcttacaac
     7021 tggtgattta cgatgcgtat cgcccacaac aagcacaggc gatgttgtgg caagcctgcc
     7081 cagacccgca atatgttgtt gatgtgacgg tcggttctaa tcacagccgt ggcacggcgc
     7141 tcgacctgac gcttcgtgat gacgtggagg tggtgaatga tgctaagcga ggaaacgtcc
     7201 gccgtacgcc cacaaaaaca aacgcgattt aacggtgcaa aactggtatg gatgctgaaa
     7261 ggcagtccgc tcaccgtgac cggcgcagtc atcattgtat taatgcgact gatgatgatt
     7321 ttttcaccgt ggctggcgac gcatgatccc aacgccattg atttaaccgc ccgccttttg
     7381 ccgccttctg cggcgcactg gtttggcacc gatgaagtgg gacgcgatct gtttagccgc
     7441 gtactggtcg gcagtcagca atcaattctc gccggattag tggtggtcgc cattgcgggt
     7501 atgattggtt cgctactcgg atgtctatcc ggtgtgcttg gcggacgcgc agacgccatc
     7561 atcatgcgca tcatggacat tatgctgtcg attccttcgt tggtactgac aatggcactg
     7621 gctgccgctc tcgggccgag tttatttaac gccatgctgg cgattgctat tgtgcgaatt
     7681 cccttttatg tgcgcttggc gcgggggcaa gcattagttg tacgccagta tacctatgtt
     7741 caggcggcga aaacctttgg cgcgtctcgt tggcatctga tcaactggca tattttacgt
     7801 aactccctac cgccgctgat cgtgcaggca tcgctggata tcggtagcgc gattttaatg
     7861 gccgccacgt tgggatttat tggcctgggt gctcaacaac cgagtgctga atggggcgcg
     7921 atggtagcga atggtcgcaa ctatgtactc gatcagtggt ggtattgcgc atttccgggg
     7981 gcagcgattt tgcttacctc cgtcgggttt aatctctttg gtgatggtat tcgcgatctg
     8041 cttgacccga aagcaggagg aaagcagtca tgacccaacc cgttctggac attcaacagc
     8101 tgcatttgag tttccccggt tttaacggtg atgttcacgc gctcaacaat gtgtccttgc
     8161 agattaaccg cggcgaaatt gtcggtctgg tgggagaatc cggctcaggt aaatcagtca
     8221 ccgcaatgct gattatgcgt ctgctaccga cgggcagtta ttgcgtacat cgggggcaga
     8281 tttcactgct gggagaagat gttcttaacg ctcgggaaaa acagcttcgt cagtggcgcg
     8341 gcgcacgagt ggcgatgatc tttcaggaac cgatgaccgc ccttaatccg acacgtcgaa
     8401 taggtcttca gatgatggac gtaatccgcc atcatcaacc aataagtcgt cgggaagcca
     8461 gagctaaagc gattgcactg ctggaagaga tgcaaatccc ggatgccgtg gaagttatgt
     8521 cgcgctatcc gtttgagctt tcaggtggta tgcgccagcg ggtaatgatt gcgccggcat
     8581 tctcctgcga gccgcaattg attattgccg gcgaaccgac gacggcgctg gacgtcacag
     8641 tacagttgca ggtactgcgt ttgcttaaac ataaagcacg cgccagtgga actgcggtac
     8701 tgttcatcag ccatgatatg gccgtggtgt cgcaactgtg cgatagcgtt tacgtgatgt
     8761 atgccggaag cgtaattgaa agcggcggga cggcagacgt tatccatcat ccccggcatc
     8821 cgtataccat tggtttgctg caatgcgcac cagaacatgg agtaccacgc cagccattac
     8881 ccgccattcc agggacggta ccaaacctca cccatttgcc tgacggctgc gcttttcgcg
     8941 atcgttgcta tgcggcaggt gcacagtgtg cgctgacagc gtgtggtgac aacaaccagc
     9001 actgcacctg ttggtatccg cagcaggagg tcataagtgt ctgacacgtt attaacgtta
     9061 cgcgacgtcc atatcaattt tccggcccgt aaaaactggc ttggcaaatc tacggaacat
     9121 gttcatgcca ttaatggtat tgatttacag atccgccgtg gtgaaacctt agggatcgtc
     9181 ggcgagtcag gctgcggcaa aagcaccctc gcacagcttt taatgggcat gctgcaaccg
     9241 agccacgggc agtacatccg ttcaggctca caacgcatta tgcagatggt gtttcaggac
     9301 ccgctctctt cgcttaatcc gcgcttaccg gtgtggcgca tcatcacaga atcgctctgg
     9361 atagctaagc gtagtagtga acaacagcgg cgagcgttgg cagaggagtt ggctgtgcag
     9421 gtgggtattc gtccggagta tctcgaccgc ctgcctcatg cgttctccgg cgggcagcgg
     9481 caacgcatcg ccattgccag agcactctct tcgcagcctg acgtgattgt gcttgatgag
     9541 ccaacctcgg cgctggatat ctccgtgcag gcgcagatcc tcaatttgct ggtaacgcta
     9601 caggaaaatc acgggctgac ttatgtgctg atttcacaca atgtctcggt gatacgtcat
     9661 atgagcgatc gggtggcggt gatgtatctc gggcagattg tagaactggg cgacgcgcaa
     9721 caggtgttga ctgcttcggc acatccatac acccgattat tgctggattc cctccccgcc
     9781 attgataaac cgctggagga agaatgagca ttacgtaaaa cggatctgcc gggaaaccgc
     9841 acgttgccgc aaggctgttt tttctacgaa cgttgccctc tggcaaccca cggatgtgaa
     9901 gtccggcaat cattagcaat aagggaggac ggacgtgacc tccggtgctg gcgggcgctg
     9961 tagagatgct aacaacgcat caggcattca cgcgcctgac gcgtcatccg gcaatgcttt
    10021 acgatttcaa ctggtaatcc agcgtaattt ccgctttcag tacctgagag accgggcatc
    10081 ctgcttttgc tttctggatg atgccgtcaa aggtagaggc atcaataccc ggcaccgcaa
    10141 cttcactctt cagtgcgatt ttcgtaatcg caaaaccggc atccacttta tccagcgaca
    10201 catcggcggt ggtatcaatt gatgttggcg tgaatcccgc ttcccccagc attaatgaaa
    10261 gcgccattga gaaacatgcg gcatgcgctg cgccaatcag ttcttcaggg ttggttcctt
    10321 tttcgccttc aaaacgcgtg ttaaatccat acggctgttg gttcagcacg ccgctctcgg
    10381 tggatactgt tcccttcccg cgtttgatat cgccttccca gtgtgcctga cctttcttat
    10441 ggattgtcat tgttgctctc ctgtgggctt aaaaaggaaa ttatagacta cgtgagaaac
    10501 agcacgagga taagcaggat attccgaata aaatacggcg aaaatattac ttctgcactt
    10561 aaattaaatt cccacaatag ataaccggga atatatatct caag
//
LOCUS       DAZPCS010000129        10466 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZPCS010000129 DAZPCS010000000
VERSION     DAZPCS010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10466
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.129"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..204
                     /gene="cspG"
                     /locus_tag="ACHUQ7_003095"
     CDS             <1..204
                     /gene="cspG"
                     /locus_tag="ACHUQ7_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFR1136394.1"
                     /translation="KMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRTLNE
                     NQKVEFSIEQGQRGPAAANVVTL"
     gene            215..403
                     /locus_tag="ACHUQ7_003096"
     CDS             215..403
                     /locus_tag="ACHUQ7_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFR1136395.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            378..608
                     /gene="ymcE"
                     /locus_tag="ACHUQ7_003097"
     CDS             378..608
                     /gene="ymcE"
                     /locus_tag="ACHUQ7_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFR1136396.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGKM
                     AYNISYLTRVPGTNTLLACCCLSRPDEVNSEY"
     gene            598..771
                     /locus_tag="ACHUQ7_003098"
     CDS             598..771
                     /locus_tag="ACHUQ7_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFR1136397.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFIAREKM
                     TGLESYDVKIKIM"
     gene            complement(820..1893)
                     /locus_tag="ACHUQ7_003099"
     CDS             complement(820..1893)
                     /locus_tag="ACHUQ7_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFR1136398.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQFLLLAMNI
                     YIAITFWYWVRYYETAGSTTFVARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSLFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKKCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(1995..4706)
                     /gene="torS"
                     /locus_tag="ACHUQ7_003100"
                     /pseudo
     CDS             complement(1995..4706)
                     /gene="torS"
                     /locus_tag="ACHUQ7_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
     gene            4789..5817
                     /gene="torT"
                     /locus_tag="ACHUQ7_003101"
     CDS             4789..5817
                     /gene="torT"
                     /locus_tag="ACHUQ7_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFR1136399.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(5790..6482)
                     /gene="torR"
                     /locus_tag="ACHUQ7_003102"
     CDS             complement(5790..6482)
                     /gene="torR"
                     /locus_tag="ACHUQ7_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFR1136400.1"
                     /translation="MPHHIVIVEDKPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDYCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHELSADLLVTQ
                     HGEGYFLAADVC"
     gene            6569..7741
                     /gene="torC"
                     /locus_tag="ACHUQ7_003103"
     CDS             6569..7741
                     /gene="torC"
                     /locus_tag="ACHUQ7_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFR1136401.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNECHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            7741..10281
                     /gene="torA"
                     /locus_tag="ACHUQ7_003104"
                     /pseudo
     CDS             7741..10281
                     /gene="torA"
                     /locus_tag="ACHUQ7_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            10278..>10466
                     /gene="torD"
                     /locus_tag="ACHUQ7_003105"
     CDS             10278..>10466
                     /gene="torD"
                     /locus_tag="ACHUQ7_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="TorD; involved in the biogenesis of torA; acts on
                     torA before the insertion of the molybdenum cofactor and,
                     as a result, probably favors a conformation of the
                     apoenzyme that is competent for acquiring the cofactor;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFR1136402.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNDLENSIAT"
BASE COUNT         2525 a         2686 c         2789 g         2466 t
ORIGIN      
        1 aaaatgactg gtttagtaaa atggtttaac gcagataaag gttttggctt tatcactcct
       61 gatgatggca gcaaagacgt tttcgtccat ttcaccgcca tccagagcaa tgaattccgc
      121 acgctgaacg aaaatcagaa agttgaattt tctattgagc aggggcaacg tggccccgcg
      181 gcagcgaacg ttgttacgct ctaaggttgc cgttattact caacatctcc atttccgctg
      241 tccatgttgt catggttcac agtaccgtac atcggcattc gatgtgacgg agcgaaaccc
      301 tttgggcgct aagtgtattt tttgtaaatc gactatgatc acctttgata acgtcgcgct
      361 gcaaatacgc actgaccatg cgccgctgga tttcacaaaa taatatcagg ctccctcgtg
      421 gagccttttt tatatctgcc ttatttttct tcaacgctgt atgtatagta agcgataacc
      481 tgttgattat tgaatctttc gggaagatgg cttataacat ttcttacctg accagggtac
      541 cgggaaccaa caccttactg gcgtgttgct gtctttcaag accagacgag gttaacagtg
      601 aatattgaag agttaaaaaa acaagccgaa acggaaatcg ccgactttat cgcgcaaaaa
      661 atcgccgagc tgaacaagaa tacagggaaa gaagtctctg aaattcgctt catcgcacga
      721 gaaaaaatga ccgggcttga aagttatgat gtcaaaatca aaataatgtg attttgtgaa
      781 catcaccccg tgcgaggtga tgttccgctt gttgctaatt tagtgaccaa tcattggcgc
      841 ttgtggaatt aagcgtcggt acaattcctc cggcaccggg ctttgccata ctcccgcata
      901 cattgcgtaa ccaatcaccg caaacataat ccccagaacc agtagcgtca ttaaccagcc
      961 agacaacgca aaggcttttt tatttgctgc aggtttttgc agtgaaaagg tcaatgttga
     1021 ggctaccgga catgactcta cgcaagtcat acagccggta cattccactg ttcgtacctg
     1081 aattaattta tctaccggga tccgtgatgg gcactttttt gcgcatttgc cacaatcgat
     1141 acaactttcg gcattgcgac gaatcttaaa cggcgacaat agcgaaacca cgcccatcag
     1201 cgcgccatat gggcaaagat aacgacacca ggcatggcga ataaacaggc tggcaatcag
     1261 caaaacggtc acgctgatta atgtcgcggt ccccatatga cgaaagaaat cgagcatttt
     1321 aacgtccatc accacgctat agggcgacaa cataaaatag tgaatcgcct gagcgggcat
     1381 caataacgcg atatagagaa ataaactcaa cagcaaatac ttcacgccgc gcagaggaat
     1441 atccagccag cggggaagga cacattgccg accaaacagt ttgttaccga gatcgccgat
     1501 taattcagaa agcgtaccaa ccgggcataa ccatgagcaa aaggcctttt tgagtaatag
     1561 actgatgacg ataaaagcga ccaataacag catcgcggcg gcgtggacgg acggtaactg
     1621 acctgttgca aggctatatt tcagattcat cagaccggca atcggtagcc agccttcgat
     1681 acctcccggt ctggcgacaa atgtcgtact acctgccgtt tcgtaatagc gcacccaata
     1741 ccagaacgtg atggcaatat aaatattcat tgccaatagt aagaattgcg tcgctttacg
     1801 ccaggtcgtg gcattgcgcc agtcattcca cggtaatttg ccgcccgtcg tgcctggccg
     1861 ccgctgccag cgggttcttt ttttctctgc catgattttg ccagtccgtt aagttgcata
     1921 ccaaatgcca ctattctagt tattcttaac tggctgatat tgattcaaat cgcgttcagg
     1981 tctttcttat tcaatcatgc ttccagagcg gcaacactgc gtgtaatttc ttcgtgtgga
     2041 aggggggcag ataatggctg ctgctccagt tgtgcgcata gctgactggc gctacgcatt
     2101 cccagactcg agcaactgct ttttagctga tgcgcggcac gctttatttt ctcgctgtcc
     2161 tggctggcgc gggcaatgtc gatttcatcg agaagcggca gggcatgttg tttaaataat
     2221 gccagccatt cgtggatctt ctccgtcccc attaactgag cgtcttcatt gagttgcgag
     2281 acatccagtg gtagatcgtt attggcttgc agttggagat agtgcgccag taactgaccg
     2341 agcacttcac gcggcaccgg tttagggata atcccgcgaa atagtgaact ggtacgctgg
     2401 cgcagcgttt cgtcaatgac atgggcgcta aagccaatca aaaccagcga cggatattgc
     2461 tgtgccagtt gccgggcaag ggtaatgccg tcgatatccg gcagatcaaa atccaccagt
     2521 gcggcagcaa acggttcgct attttgcagt gtctctaaag cctgcgcggc attgccaata
     2581 gcaacaacct gcgcaccact ggtgttcagc atctcaacgg taattcgctg ggttagcggg
     2641 ttatcttcaa ttaacagcaa acgtaaaccg tcaagacgca ccgcctgatt ggctgttttt
     2701 ggcacgggtg cagtggcaac acgtaacggc aagcgtaaac aaaaacagct tccaacctcc
     2761 ggcgtgctgg tggcgctcag ttcgccgccc atcgcctggg ccagacggct actgatagtc
     2821 agccccagcc cggtgccgcc gcgtttgccg cttacctgca caaatggctg gaagatttct
     2881 gccagtttcg cgggatcaat accgcagccg ctgtcttcca cttcgaccag ccattgctcg
     2941 ccatcagtgc gactacgcag gataatgtgc ccttcgtcag taaaacgcag ggcattgctc
     3001 aataggttgg taatgacctg acgaatacgt cgtggatcgc ccattaacgc gctcggtaca
     3061 tcatcggcaa ttgccgttgc caggcgaatc gggcgacctt tcacccgtcc gctcattaat
     3121 tgcagggtac tttccagcag cgggcgcggt tcaaagggct catcgctgac tgaaacattt
     3181 ttgccacctg cttcgatagc cgaataatcg agaatatcgt tgaggatggt cagcaacgat
     3241 tcgccagagt cggtaatcgc ctgcaaatca tcacgctggg cgttaagtgc ggggttatct
     3301 gccagcagtt gagcagtgcc gagaataccg tacagcggtg tgcggatctc atggctcatc
     3361 gccgtcagaa acgccgattt tgcctggctg gctttttctg cttccgcccg tgcctgtcgg
     3421 tgttctatca ccagttcctg caattcagct gtacgcgctt tgacctgcgc cgccagctgt
     3481 tcgcggtggc gattcagtgc atgaacactg ctgcgaaacg catccatcag ccgcccgatg
     3541 gtatccagct cccgtacgcc agcggtttcc gggaaagggg agtcaatatc accgtccagc
     3601 agccgttgca gcgcctgcgt ttgttcggca agtggacgcg tgaatgagcg ataaaccacg
     3661 cgccagagga tcataatcag tgcgcaaagt gaaaccatcc ccagcaataa caggctgtat
     3721 tgcccgcgtg cactggcttt ttccagatgt gccagtccat gctgattgtg cagctcaatg
     3781 gtgtcgacca gctgactgac ttcgctacta aactgcgcga actgggcgat attattttgg
     3841 gcgagagttt gcaggtgatt gctgatttca ctgtcctgct ggaacagcgc caacagatcg
     3901 ctatattggc taacggtagt taacgttgtt gcgacctgcg cacgaactcc cggatcttca
     3961 atgcgtattt gccgacgttg cagaattttc accgcattat tgagctgctt ttccagcgtt
     4021 ggtgcatttt tctggatctg ctccagcccc agattcatca ccatttgctg cacccgcaga
     4081 gcgctaaggc gcagttcatt catctggtta acatactcaa gatcgatatc aatcagccga
     4141 tcgagtgcac tttcagcagc ctgacgctga tcttgttcga tcaaatcgta aatcccggcc
     4201 tgggtcgctc cagcggaagt tgtcgcatta ttcgcctgac cttgctccag gcgtgcgatc
     4261 tcatcggcgg cagctatctg ctgactgagt tgccgttgtt gctggcgtag ttgcagacgc
     4321 cgccccacca gttccccttg ctgacgtaat gaacgggaga tctcctgctc ctgttgttca
     4381 atagcggtgg tatcaaaacc ttgttcccgt aacgcttgca gcaacgcatt aatcttcagg
     4441 ctttatgcgg tgagcattcg cccctgcgcc tgccacatct tttcgttatc ggcactggtc
     4501 aggttctgcg cggcgaaaag ttcccaggcg ctggcttcgc tcaactggcg cgccatattc
     4561 atggtaggaa tcaatgcctg agtattgtct ttttccacct ggctgataaa gcgcaggttg
     4621 taccatccca ccagggtact ggtcagggtt aacagcgcca tcagggcaaa gcccatccag
     4681 agtcttcggg tcagggttaa attcacggtc ggtgcactta aggtgaaaaa ggttgagtcg
     4741 caaagtggaa tgcatctagc ataaagcctt attattgatg aggctatcat gcgcgtactg
     4801 ctatttttac ttctttccct tttcatgttg tcggcatttt cggctgataa cctgttgcgc
     4861 tggcatgatg cgcagcattt cacggtgcaa gcctctatgc cgcttaaagc caaacgcgta
     4921 tggaaactgt gcgcgcttta tcccagcctg aaagattcat actggttatc gttgaactat
     4981 ggtatgcagg aggctgctcg ccgctacggt gtggatttaa aagtgctgga ggcaggcggc
     5041 tacagccagt tggctaccca gcaagcacaa atcgaccagt gtaaacagtg gggcgcagag
     5101 gccattttgc tcggtagtag cacgacgtca tttcccgacc tgcaaaagca ggtagcaaat
     5161 ctgccggtga tcgaactggt aaatgctctt gatgatcccc aggtgaaaag ccgcgttggt
     5221 gtaccctggt ttcagatggg ctatcaacct gggcgatatt tggtgcaatg ggcgcacggt
     5281 aaaccactga acgtgctgct gatgcccgga cccgataacg ccgggggcag taaggagatg
     5341 gtcgagggtt ttcgcgcagc cattgccgga agcccggtgc gtattgttga tattgcgctt
     5401 ggtgataacg atattgaaat ccagcgcaac ctgttgcagg agatgctgga acgccaccca
     5461 gaaatcgacg tcgttgccgg aacggccatt gcggcagagg cggcaatggg tgaagggcgt
     5521 aacctgaaaa cgccgcttac cgtggtgtcg ttttatcttt cacatcaggt gtatcgcggg
     5581 ctgaagcggg gaagagtgat tatggctgcc agcgatcaaa tggtctggca gggggaactg
     5641 gcagttgagc aggccatcag gcaattacag gggcaatcgg tgtctgataa tgtcagccca
     5701 ccgattttag ttctgacgcc gaaaaatgcc gaccgagaac atatccgccg ctcgctgtca
     5761 ccggggggat ttcgtccggt ctatttttat cagcacacat cagcggctaa gaaataacct
     5821 tcaccatgtt gcgtcaccag taaatccgcg ctgagttcat gacgtaaacg acgaattaac
     5881 acatcgacgg tgcgcaggtc agggttttcc acccgacgcg cagaaagcat acgcagcaga
     5941 cgctcacggc tgagaatttc gcccggatta gtcacaaatg ccaccaacat ttcatactct
     6001 gcgcgggtca gtttaatcgg ctcgccatcc cgctccagcg tatggcgcga cacattcagg
     6061 caataacccg caaagcgata gcagtagtcc tgagtgtgcg gttgagcttg tcgcgcgagg
     6121 tcgattcgcc agagcagatt tttcacccgt actaccagtt cgcgcagttc cagcggtttg
     6181 gtgacgtaat cgtctgcgcc catttccagc ccaacaatac ggtcaatccg atcgctgcgt
     6241 ccggtaacca gaataatccc caccgttgag cgttctcgca gggcgcgggt taacatcagg
     6301 ccattttcat cgggtaagtt gatatccagc agaattaaat ctaccggctg attctgcata
     6361 atttcccgta gcccggcacc gctcgccgta acggaaacgg tatacccctc ctgagtgaag
     6421 taggattgta atcgcgcctg ggtaaccggc ttatcttcaa caataacaat gtgatgtggc
     6481 atcagagggt tttactcatt ctgttcatat ctgttcatat ttgctcatta agatcgcttc
     6541 actaaaccat aattctacag gggttattat gcggaaactc tggaacgcgc tacgccgacc
     6601 cagtgctcgt tggtcggtac tggcgctggt cgcaattggg attgtgattg gcattgcgct
     6661 gattgtattg ccacacgttg ggatcaaagt caccagcaca accgaatttt gtgtcagttg
     6721 ccacagtatg caaccggtgt atgaagaata taaacagtcg gtgcatttcc agaacgcctc
     6781 cggcgtgcga gctgaatgcc atgactgtca tatcccgccg gatattccag gcatggtgaa
     6841 gcgcaaactg gaagcgagca acgacattta ccagaccttt attgcccact ccattgatac
     6901 acctgaaaaa ttcgaagcca aacgcgcgga acttgccgag cgtgaatggg cgcgaatgaa
     6961 agaaaacaac tcggcaacct gtcgctcctg ccataactac gatgcgatgg atcatgccag
     7021 gcagcatcct gaagcggcac gtcagatgaa ggtggcagcg aaagataatc aatcctgcat
     7081 cgactgtcat aaaggtattg cccaccagtt accggatatg agtagcggct tccgtaagca
     7141 gttcgatgag ctgcgcgcca gtgctaatga cagtggtgac acgctgtact ctattgatat
     7201 taagccaatt tatgcggcga aaggcgataa agaagcctct ggttctctgc tgcctgcttc
     7261 ggaagtgaaa gtccttaaac gtgacggcga ctggctgcaa attgaaatta ccggctggac
     7321 ggaaagcgcc ggacgtcagc gtgtactcac ccaattccca ggtaaacgca tctttgttgc
     7381 ctcgattcgt ggtgatgtgc agcagcaggt gaaaacgctg gagaaaacca ccgttgccga
     7441 caccaatacc gagtggagca agttgcaggc caccgcgtgg atgaagaaag gcgacatggt
     7501 gaacgatatc aaaccgatct gggcctatgc ggattcgttg tacaacggca cctgtaacga
     7561 gtgccacggc gcaccggaaa tcgcccactt tgacgctaac ggttggatcg gcacgctcaa
     7621 cggcatgatt ggctttacca gtctcgataa acgtgaagaa cgcactttgt tgaaatatct
     7681 gcaaatgaat gcgtctgaca ccgcaggtaa ggctcacggc gataagaagg aagaaaaata
     7741 atgaacaata acgatctctt tcaggcatca cgtcggcgtt ttctggcaca actcggcggc
     7801 ttaaccgtcg ccgggatgct ggggccgtca ttgttaacgc cgcgccgtgc aactgcggcg
     7861 caagcggcga ctgaggctgt catctcgaaa gagggcattc ttaccgggtc gcactggggg
     7921 gctatccgcg cgatggtgaa ggatggtcgc tttgtggcgg caaaaccgtt cgaactggat
     7981 aaatatccgt cgaaaatgat cgccggattg ccggatcacg tacacaacgc ggcacgtatt
     8041 cgttatccga tggtacgcgt ggactggctg cgtaagcgcc atctgagcga cacctcccag
     8101 cgcggtgata accgttttgt gcgcgtgagc tgggatgaag ccctcgacat gttctatgaa
     8161 gaactggaac gcgtacagaa aactcacggg ccgagtgcct tgctgaccgc cagtggttgg
     8221 caatcgacgg ggatgttcca taacgcttcg gggatgctgg cgaaagctat tgccttgcat
     8281 ggtaatagcg ttggtacggg cggagattac tctaccggtg ctgcgcaggt gatcctgccg
     8341 cgcgtagtcg gttcaatgga agtgtatgaa cagcaaacct cctggccgct ggtattgcag
     8401 aacagcaaaa ccattgtgct gtggggctcc gatttgctga aaaaccagca agcgaactgg
     8461 tggtgcccgg atcacgatgt ttatgaatat tacgagcagc taaaagcgaa agtcgccgcc
     8521 ggtgaagttg aggtcatcag catcgatccg gttgtcacat ccacccatga gtatctgggg
     8581 cgcgagcatg tgaagcacat tgcggttaac ccgcaaactg acgtgccgct gcaactggcg
     8641 ctggcacata cgctgtacag tgaaaacctg tacgacaaaa acttcctcgc taactactgt
     8701 gtgggttttg agcagttcct gccgtatctg ctgggtgaga aagacggtca gccgaaagat
     8761 gccgcatggg ctgaaaaact gaccggcatt gatgccgaaa ccattcgtgg gctggcgcgg
     8821 cagatggcgg cgaacagaac gcaaattatt gctggctggt gcgtgcagcg tatgcagcac
     8881 ggtgaacagt gggcgtggat gattgtggtt ctggcggcga tgctggggca aattggcctg
     8941 ccaggtggtg gttttggctg gcactacaac ggcgcaggca cgccggggcg taaaggcgtt
     9001 attctgagtg gtttctccgg ctctacgtcg attccgcctg ttcacgacaa cagtgactac
     9061 aaaggctaca gcagcactat tccgattgcc cgttttatcg atgcgatcct cgaaccgggg
     9121 aaagtgatca actggaacgg taaatcggta aaactgccgc cgctgaaaat gtgtattttt
     9181 gccggaacta acccattcca tcgccatcag cagatcaacc gcattattga aggctggcgc
     9241 aagctggaaa cggttatcgc catagataac cagtggacct caacctgccg ctttgccgat
     9301 atcgtgctgc ctgcgaccac gcagtttgag cgtaacgatc tcgaccagta cggcaaccac
     9361 tccaaccgtg gcattatcgc catgaaacag gtggtgccgc cgtagttcga ggcgcgcaac
     9421 gactttgata ttttccgcga gctgtgccgt cgctttaatc gtgaagaagc ctttaccgaa
     9481 gggctggacg aaatgggctg gctgaaacgc atctggcagg aaggtgtaca acaaggcaaa
     9541 ggacgcggcg ttcatctgcc agcgtttgat gacttctgga ataacaaaga gtacgtcgag
     9601 tttgaccatc cgcagatgtt tgttcgccac caggcattcc gcgaagatcc ggatctcgaa
     9661 ccgctgggca cgccgagtgg cctgattgag atctactcga aaaccatcgc cgatatgaac
     9721 tacgacgatt gtcaggggca tccgatgtgg tttgagaaaa tcgaacgctc ccacggtggg
     9781 cccggctcgc agaagtatcc gttgcatctg caatctgtgc atccggattt ccgacttcac
     9841 tcgcagctat gtgagtcgga aacgctgcgt cagcaatata cggtagcggg taaagagcca
     9901 gtgttcatta acccgcagga tgccagcgcg cgcggtattc gtaacggtga tgtggtacgc
     9961 gtctttaacg ctcgcggtca ggtgttggca ggagcagtag tttctgaccg ctatgcaccc
    10021 ggcgtggcgc gaattcacga aggggcatgg tacgatccag ataaaggcgg cgagcctggt
    10081 gcgctgtgca aatacggtaa ccccaacgtg ctgaccatcg acatcgggac ttcgcagctg
    10141 gcgcaggcga ccagtgcgca cactacgctg gtggaaattg agaagtacaa tggaacagtg
    10201 gagcaggtga cggcgtttaa cggccccgtg gagatggtgg cgcagtgcga atatgttccc
    10261 gcgtcgcagg tgaaatcatg accacgctga cagcacaaca gattgcctgt gtttacgcct
    10321 ggctggcgca gttgttctcc cgtgaactgg acgatgaaca actgacgcaa atcgccagtg
    10381 cgcagatggc tgaatggttt tcgttgctga aaagcgaacc gccgctcact gcggcggtga
    10441 atgatctgga aaacagtatt gccacg
//
LOCUS       DAZPCS010000130        10420 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZPCS010000130 DAZPCS010000000
VERSION     DAZPCS010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10420
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.130"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            2..1369
                     /gene="dcuC"
                     /locus_tag="ACHUQ7_003106"
     CDS             2..1369
                     /gene="dcuC"
                     /locus_tag="ACHUQ7_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFR1136403.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASCAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFVNVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            complement(1412..1909)
                     /gene="sspB"
                     /locus_tag="ACHUQ7_003107"
     CDS             complement(1412..1909)
                     /gene="sspB"
                     /locus_tag="ACHUQ7_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFR1136404.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(1915..2553)
                     /gene="sspA"
                     /locus_tag="ACHUQ7_003108"
     CDS             complement(1915..2553)
                     /gene="sspA"
                     /locus_tag="ACHUQ7_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFR1136405.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(2948..3340)
                     /gene="rpsI"
                     /locus_tag="ACHUQ7_003109"
     CDS             complement(2948..3340)
                     /gene="rpsI"
                     /locus_tag="ACHUQ7_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFR1136406.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(3356..3784)
                     /gene="rplM"
                     /locus_tag="ACHUQ7_003110"
     CDS             complement(3356..3784)
                     /gene="rplM"
                     /locus_tag="ACHUQ7_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFR1136407.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(4003..5130)
                     /gene="zapE"
                     /locus_tag="ACHUQ7_003111"
     CDS             complement(4003..5130)
                     /gene="zapE"
                     /locus_tag="ACHUQ7_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFR1136408.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            5324..5722
                     /gene="zapG"
                     /locus_tag="ACHUQ7_003112"
     CDS             5324..5722
                     /gene="zapG"
                     /locus_tag="ACHUQ7_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFR1136409.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQVPRDYSEGASGLLRTGAKRD"
     gene            5876..7223
                     /gene="degQ"
                     /locus_tag="ACHUQ7_003113"
                     /pseudo
     CDS             5876..7223
                     /gene="degQ"
                     /locus_tag="ACHUQ7_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
     gene            7313..8380
                     /gene="degS"
                     /locus_tag="ACHUQ7_003114"
     CDS             7313..8380
                     /gene="degS"
                     /locus_tag="ACHUQ7_003114"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFR1136410.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(8442..9380)
                     /gene="mdh"
                     /locus_tag="ACHUQ7_003115"
     CDS             complement(8442..9380)
                     /gene="mdh"
                     /locus_tag="ACHUQ7_003115"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFR1136411.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKNCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGKEFVNK"
     gene            9815..10285
                     /gene="argR"
                     /locus_tag="ACHUQ7_003116"
     CDS             9815..10285
                     /gene="argR"
                     /locus_tag="ACHUQ7_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFR1136412.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
BASE COUNT         2548 a         2654 c         2611 g         2607 t
ORIGIN      
        1 aatgttcggc ataattatat ctgtcatcgt attaattacg atgggctatt tgatcctgaa
       61 aaactacaaa cctcaggtgg tgctggctgc cgcaggtgtc ttcctgatga tgtgcggagt
      121 ctggttaggg ttcggtggtg tactcgatcc cgccaaaagc agcggctact tgatcgtcga
      181 catttataat gaaatcctgc gcatgctgtc caaccgcatt gccggattgg ggctgtcgat
      241 tatggcggtg ggcggttatg cccgctacat ggagcgcata ggagccagtt gcgcgatggt
      301 gagcttgcta agccgcccgt taaaactcat tcgctcgccg tatattattc tgtcggcaac
      361 ttacgtcatc ggccaaatca tggcgcagtt tattaccagc gcctccggtc tgggtatgtt
      421 gctgatggtc accttatttc cgacgctggt gagtctggga gtaagtcgcc tctctgcggt
      481 ggcggttatc gcaaccacga tgtccattga gtgggggatt ctggaaacga actccatttt
      541 tgcagcccag gtcgcgggaa tgaaaattgc cacatacttc ttccactacc agcttccggt
      601 cgcctcttgc gtcattatct cggtggcgat ctcccacttt ttcgtgcaac gcgcttttga
      661 caaaaaagat aaaaatatca atcacgaaca ggcagagcaa aaaactctcg ataatgtccc
      721 gccgctctat tacgccattt tacctgtgat gccgttaatc ctgatgctcg gctcgctgtt
      781 cctcgcccac gtcgggctga tgcagtcaga actgcatctg gtggtggtga tgttactaag
      841 tttgacggtg acgatgtttg ttgagttctt ccgcaagcat aacttgcgcg aaacaatgga
      901 cgatgtgcag gcgttttttg acggcatggg tacgcagttt gtcaacgtgg taacgctggt
      961 ggtcgcgggt gaaatatttg cgaaaggctt aacgacgatt ggcactgttg atgcggttat
     1021 caggggtgcg gagcattctg gtctgggcgg tattggcgtg atgattatta tggcgctagt
     1081 cattgccatt tgtgccattg tgatgggctc tggcaatgcg ccgtttatgt catttgccag
     1141 tcttattccg aatatcgcag ccggactaca tgtaccagcg gttgtaatga ttatgccgat
     1201 gcattttgcc acgacgctag cgcgcgcggt ttcgccgatt actgcggtgg tggtcgttac
     1261 gtcaggaatt gcaggcgttt cgccttttgc ggtggtgaag cggacagcga tccccatggc
     1321 agtcggtttc gtggtgaata tgattgccac aatcacgcta ttttattaag tcattaaaaa
     1381 gacaaaacag gccgcctggg cctgttttgt attacttcac aacgcgtaat gccggtcgac
     1441 caccgcgtgg tggctgcgga ggttcatcgt caggatgagt gtcatcatcg tgatctggct
     1501 tgtcgccatc aataaccgac ataacggttt cgttgtctgc cgatgcctct tcatcattca
     1561 tgatgctggt atcttcatcg taggcagctt caggctcaaa catcgtgcct gcgccatttt
     1621 cacgggcgta gatagccagc acggcagcca gcggcacaga aacctgacgc ggaatgccac
     1681 caaagcgcgc gttaaagcgc acctcatcat tcgccagttc cagattgccg acagcacgcg
     1741 gcgcaatgtt gagtacgatt tgcccgtcac gcgcatattc cataggaacc tgcacgccag
     1801 ggagcgtcac atccaccacc aggtgcggcg tgagctggtt atccagcaac cactcataga
     1861 atgcacgcag cagataggga cgacgtggtg ttagctgtga caaatccata cagattaact
     1921 ccggcccaga cgcatttcac gttctgcttc agttaaagaa gcaaggaaag agtcacgctc
     1981 aaagacgcgg gtcatatagc ctttcagctc tttcgcaccc gggccgctga actcgatgcc
     2041 cagttgcggc agacgccaca gcagcggagc aaggtagcaa tcgaccaggc tgaactcatc
     2101 gctcaggaag tacggcttct gaccgaagac cggcgcaatc gccagcagtt cttcgcgcag
     2161 ttgcttacgt gcggcatctg cttcagaagc tgaaccgttg atgatagtgt tcatcagcgt
     2221 gtaccagtct ttttcgatgc gatgcatgta cagacggctt tcaccgcgag ctaccgggta
     2281 aacaggcatc agtggcggat gcgggaaacg ctcatccaga tattccataa tgatgcgaga
     2341 ttcccacagg gtcagctcac gatccaccag ggtcggaacg ctctgattcg ggttgaggtc
     2401 aatcagatcc tgaggcggat tgtccttttc cacgtgttcg atctcgaaac ttacaccttt
     2461 ctcagccagc acaatgcgga cctgatggct atagatgtca gtaggaccgg aaaacagcgt
     2521 cattaccgaa cgtttgttgg cagcgacagc catgaaaacc tccaggtata gtcagaattt
     2581 ttactgctac cagccaccag gtggccagtc agaagttgtg ttacccaata aggaacgact
     2641 ctctttgttc gaaaatcaaa caaaaaatga gcaatacccg acatttgggc agaaaattgg
     2701 atgatagttt accagatttt gcgacctttg tggtgagtgg atgccggaaa tggggaaaaa
     2761 gagatgcgct ttagtctgaa atagttgact tagaccctta ttggcgatgt ggtttttgtt
     2821 ttacctgtct ttcagacggc agcaaaaagc aacttcccat tttttacgct gattcagaat
     2881 ttagccataa aaaaacccgc cgaagcgggt tttttcgcaa attgttttct gccgtagcag
     2941 aagccaatta acgtttggag aactgcggac gacgacgtgc tttacgcaga ccgactttct
     3001 tacgttcaac ctgacgagcg tcacgagtaa cgaagccagc tttacgcagt tcagaacgca
     3061 gggactcgtc gtattccatc agagcgcggg tgataccgtg acggatcgca ccagcctgac
     3121 cagagatacc accaccttta acggtgatgt acaggtccag tttctcaacc atgtcgacca
     3181 gttccagcgg ctgacgaact accatgcggg cagtttcacg accgaagtac tgttccagag
     3241 aacgttggtt gattacgatt ttaccgttgc ccggtttgat gaaaacgcga gctgcggaac
     3301 ttttgcggcg accagtgccg tagtattgat tttcagccat tgcctataat cccgattaga
     3361 tgtcaagaac ttgcggttgc tgtgccgcgt ggttgtgctc gttacccgcg taaactttca
     3421 gtttacggaa catagcacga cccagcgggc cttttggcaa catgccttta accgcgattt
     3481 caatcacacg ctcaggacgg cgagcaatca tctcttcaaa ggtcgcttgt ttgataccac
     3541 cgatgtggcc ggtgtggtga tagtacactt tgtcagtacg cttgttgccg gttacagcaa
     3601 ctttgtcagc gttcagaacg atgatgtaat caccggtatc tacgtgcgga gtatattccg
     3661 ctttgtgctt accgcgcagg cgacgagcca gttcagtagc cagacggccc agagttttac
     3721 cggtcgcgtc aacaacatac cagtcgcgtt ttacggtttc tggtttagct gtaaaagttt
     3781 tcattaaaag cttacccaat aaatagttac acgttggtga acacccaaac gtcttcaatt
     3841 gttgaggttc acacgacaaa gtccggcaaa cctacccctt cgaatagcct atgccagcac
     3901 acaaaaagtt ttgggaaaaa aactttcttg taacgtgggg tcgcaggatt atagagaagt
     3961 cggggtcaaa gatcgacccc tttttgtgat ttgtggcagg ttttaacccg ccaaatgctc
     4021 gcgcttcaga tactcttcgc tttgcatctc ttgcagacgt gacaggcaac gctggaactc
     4081 aaacttcagc cgctcaccct gataaatttc atacagcggc acttctgcac tcaccactaa
     4141 tttgacatgg cgctcgtaaa actcatccac cagcgcaata aagcgccgcg cttcgctctc
     4201 catcaaccgc gtcatgactg gcacatcaaa caacattact gtgtgaaaca ggcgtgagag
     4261 cgcaatatag tcatgctgac tgcgggcgtc gacgcacagc gtagtaaaag aaaccgccag
     4321 cgtctggttc tcgacgccca ttgtcgccaa tggccgatgg ttgatttcta acgtcggtga
     4381 attttctcgt ttcacccccg ccagcgccaa ccatagttta tccatctgcg cccgggtttc
     4441 atcgttgagt ggcgaaagcc acagatgcgc ctgagtgagt gtacgcagtc gataatcaac
     4501 accagcgtcc acgttcatta catcacaatg ctgtttaatg gcatcgattg caggcagaaa
     4561 acgcgcacgt tgcaggccat ttcgataaag ttcgtccggc ggaatatttg acgtcgctac
     4621 cagggtaata ccgcgggtga acagggcttt catcagaccg ccaagtagca tggcgtcggt
     4681 aatatcagaa acaaaaaatt cgtcaaaaca gagcacgtca gtttcggctt taaagcgatc
     4741 ggcaataatt tccagcggat cggtctgccc ctgtaaggca gttagctctt cgtgcacccg
     4801 cagcataaaa cggtgaaagt gcaggcgctg tttccgctct cccggcaggc tttgatagaa
     4861 aaggtccatc agccaggttt tcccgcgtcc tacaccgccc cacatatata agccacgcac
     4921 tggcgtatgc tttgtgtctt cgcgtttacc ccacagctta ccgacccgcg ccattagccc
     4981 actcgtcctg ggggctggtg gcctgctatt gatgagttct tgataaataa tttccaggcg
     5041 gctgacggcc tctttttgaa cgtcgtcggg ttgatggctg ccttcattga gcgccttcag
     5101 gtattgcgat gttggggtaa cgctttgcat gatcttattg ttattccttg aataatcggt
     5161 gcgccgttgt tcacggttga cgaaaaaaag gccgttctac actacgcgat atgcagtcgg
     5221 gattccactt ctgtggaatt aacggttata gtggcataat cagccgcagg catggagcct
     5281 gaagccaaca ccctacggaa acaaaagaca acgggagatg ttcatgacct gggaatatgc
     5341 gctaattggg ttagtcgtcg gcatcattat tggtgctgtg gccatgcgtt ttggtaatcg
     5401 taaactacgc cagcaacagg cgttgcagta cgaactggaa aagaataaag ctgaactgga
     5461 cgagtatcgc gaagagctgg ttagccactt tgcccgcagc gcggaattac tggataccat
     5521 ggcgcacgac tatcgccagc tgtatcagca catggcaaaa agctccagca gcctgctgcc
     5581 ggaactgtct gctgaagcaa acccgttccg taatcgtctg gcagagtctg aagccagcaa
     5641 cgatcaggca ccggtgcagg tgcctcgcga ctattctgaa ggcgcatccg gcctgctgcg
     5701 tactggcgcg aagcgcgact aatttatttt tcgggcgcag ccattgcgcc ctcctcttct
     5761 ctccctcccc gactatcatt taatctggtg tctcattgtt agccgtctga aaattcaata
     5821 acatcaaact gttttgaatc tcttttctta tcattcaggt acgagagcag gaataatgaa
     5881 aaaacaaacc cagctgttga gtgcattagc gttaagtgcc gggttaactc tctcggcgtc
     5941 atttcaggcc gtcgcgtcga ttccaggcca ggttgccgat caggcccctc tccccagtct
     6001 ggctccaatg ctggaaaaag tgcttccggc agtggtgagc gtacgggtgg aaggaacggc
     6061 cagtcaggga cagaaaatcc cggaagaatt caaaaagttt tttggtgatg atttaccgga
     6121 tcaacctgca caacccttcg aaggtttagg ttccggtgtc atcatcaacg ccagtaaagg
     6181 ctatgtgctg accaacaacc atgtgattaa tcaggcacag aaaatcagta ttcagctcaa
     6241 tgatgggcgc gagtttgatg caaaactgat tggtagcgat gaccagagcg atatcgccct
     6301 gttacaaatt caaaacccga gcaaattaac gcaaatcgct attgccgact ccgataaatt
     6361 gcgcgtcggt gattttgccg tagcggtcgg taacccattt ggccttgggc aaaccgccac
     6421 ctctggcatt gtttccgcat taggccgcag cgggttgaat cttgaaggtc tggaaaactt
     6481 tatccagaca gatgcttcca ttaaccgcgg taactccggc ggtgcactat taaaccttaa
     6541 cggtgagtta attggcatca acactgcaat ccttgcgcct ggcggcggga gcgtcgggat
     6601 tggatttgcc atccccagta atatggcgcg aacactggcg cagcagctta tcgactttgg
     6661 tgaaatcaaa cgcggtttgt taggcatcaa aggcaccgag atgagtgccg atatcgccaa
     6721 agccttcaac cttgacgtgc agcgtggcgc gtttgtcagc gaagtgttgc caagttctgg
     6781 ctcagcgaaa gcgggcgtca aagcgggcga tattattacc agcctcaacg gcaaaccgct
     6841 gaatagcttt gctgagttgc gctctcgtat cgcgactaca gagccgggca cgaaagtgaa
     6901 gcttggcctg ctgcgtaacg gcaaaccact ggaagtagaa gtgacgcttg ataccagcac
     6961 ctcttcgtcg gccagcgctg gaaggtgcaa cgttgagcga tggtcagcta aaagatggcg
     7021 gcaaaggtat taagatagat gaagttgtca aaggaagccc agctgctcag gctggcttgc
     7081 aaaaagacga tgtgatcatt ggcgtcaacc gcgatcgggt gaactcgatt gctgaaatgc
     7141 gtaaagtgct ggcggcaaaa ccggccatca tcgccctgca aattgtacgc ggcaatgaaa
     7201 gcatctatct gctgatgcgt taatgtcgta aaccgggcat caggctgacg tgtgatgtcc
     7261 ggttaactcg tggtatgctg ctgccgttcc cttttttaat gacgcctcca tcatgtttgt
     7321 gaagctctta cgttccgttg cgattggatt aattgtcggc gctattctgc tggttgccat
     7381 gccttcgctg cgcagcctta acccgctttc cactccgcaa tttgacagta ccgatgagac
     7441 gcctgccagc tataatctgg cggttcgccg cgccgcgcca gcggtggtta acgtttacaa
     7501 ccgtggtttg aacaccaact ctcacaacca gcttgagatc cgcaccctgg gatccggtgt
     7561 aatcatggat caacgcggtt atatcatcac caataaacac gtcatcaacg acgccgatca
     7621 gatcatcgtc gccttacagg atggacgtgt atttgaagca ttgctggtgg gatctgactc
     7681 tctaaccgat ctggcggtac ttaaaattaa tgccactggc ggtttaccta ccattccaat
     7741 taatgcacgt cgcgtaccgc acattggcga cgtagtactg gcgatcggta acccgtacaa
     7801 cctcgggcag accattaccc aagggattat tagtgccacg ggtcgaatcg gtctgaaccc
     7861 gaccgggcgg caaaacttcc tacaaaccga tgcttccatt aaccacggta actctggcgg
     7921 cgcgctggtg aactcgctgg gagaactgat gggcattaac acgctgtcgt ttgataagag
     7981 taacgatggc gaaacgccgg aaggtatcgg ctttgcgatt cctttccagt tagcaaccaa
     8041 aattatggat aagctgatcc gcgatggtcg cgtgatccgc ggctacattg gtatcggcgg
     8101 acgcgagatc gcaccactgc acgcgcagag cggtggtata gatcaactgc aagggatcgt
     8161 ggttaatgaa gtgtcacctg acggcccagc ggcgaatgcg ggtattcagg tcaacgatct
     8221 gattatttcg gtggataaca aaccggccat ctctgctctg gagacgatgg atcaggtggc
     8281 ggaaattcgc cctggttcgg tgatcccggt tgtagtgatg cgtgatgata agcagttaac
     8341 gctgcaggtc accattcagg aatatccggc aaccaattaa gtcgagtgct caaaacaaaa
     8401 accggagtct gtgctccggt tttttattat tcgctaatca attacttatt aacgaactct
     8461 ttgcccaggg cgatatcttt cttgagcgta tccagcatac cttccagcgc gttctgttca
     8521 aatgcgctca gggtaccgat agatttacgc tcttccacgc cgtttttacc cagcagcagc
     8581 ggttgagaga agaaacgggc gtactgaccg tcgccttcaa cgtaggcaca ttcgacaacg
     8641 ccttgttcgc cctgcagtgc acgaaccaga gacagaccaa aacgtgcagc tgcctggccc
     8701 atagacaggg ttgcagaccc gccaccggct ttcgcttcaa ccacctcagt acccgcgttc
     8761 tggatacgtt tggtcagatc agccacttcc tgctcggtaa aactaacgcc aggaacctgt
     8821 gacagcagcg gcagaatggt aacaccagag tgaccgccaa taaccggcac ttcaacttcg
     8881 cctggctgtt tgcctttcag ttccgcaaca aaggtgttgg aacgaatgat atccagcgtg
     8941 gtaacgccga acagtttgtt tttgtcataa acaccggctt ttttcagcac ttcagcagca
     9001 attgcaactg tggtgttaac cgggttagtg ataataccaa tgcacgcttt cgggcagttt
     9061 ttcgcaactt gctgtaccag gtttttcacg atgccggcgt taacgttaaa caggtcggaa
     9121 cgatccatac ccggtttacg cgctacgcct gcagagataa gaacgacatc tgcgccttcc
     9181 agcgccggag tcgcatcttc accagaaaaa cctttgattt tcacagcagt agggatatgg
     9241 ctcagatcga cggccacacc gggagtcact ggagcgatat catacagaga gagttctgaa
     9301 cctgaaggca gttgggtttt taacagtagt gcaagcgcct ggccaatacc gccagcagcg
     9361 ccgaggactg cgactttcat cctaaactcc ttattatatt gataaactaa gatatgttgc
     9421 tccgctgccg cgaccttaat ccacaaaatt gccgtttaca atgaccacat ctcaagaatg
     9481 tgtagtcacg caagtttagc gtttatgcat ttaattgccg taatcaggaa cctaacttac
     9541 gtaattaaca gtcgcttatg cattagcgca acattccaac aggtggtgac aatataccct
     9601 accgttcagc caaaacaaca tcaatttgat aacaattaat ttacttttaa acagaatttg
     9661 catgccgtga cgcaggcatg tttctcaata acgaaatttg ataaaatccc gctctttcat
     9721 aacattattt cagccttctt cagggctgac tgtttgcata aaaattcatc tgtatgcaca
     9781 ataatgttgt atcaaccacc atatcgggtg acttatgcga agctcggcta agcaagaaga
     9841 actagttaaa gcatttaaag cattacttaa agaagagaaa tttagctccc agggcgaaat
     9901 cgtcgccgcg ttgcaggagc aaggctttga caatattaat cagtctaaag tctcgcggat
     9961 gttgaccaag tttggtgctg tacgtacacg caatgccaaa atggaaatgg tttactgcct
    10021 gccagctgaa ctgggtgtac caaccacctc cagtccattg aagaatctgg tgctggatat
    10081 cgactacaac gatgcagttg tcgtgattca taccagccct ggcgcggcgc agttaattgc
    10141 tcgcctgctg gactcactgg gcaaagcaga aggtattctg ggcaccatcg ctggcgatga
    10201 caccatcttt accacccctg ctaacggttt caccgtcaaa gacctgtacg aagcgatttt
    10261 agagctgttc gaccaggagc tttaatctct gccccgtcgc ttctggcggc ggggaaaatg
    10321 ttgcttatcc ctctcattcc ccttctttct cctgcgatac atttaacgaa tagtgcgttt
    10381 taccgcgaca catcattcac aaaatgaata catacggtaa
//
LOCUS       DAZPCS010000131        10208 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZPCS010000131 DAZPCS010000000
VERSION     DAZPCS010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10208
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.131"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..624
                     /locus_tag="ACHUQ7_003117"
     CDS             <1..624
                     /locus_tag="ACHUQ7_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1136413.1"
                     /translation="CLLRAILVLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALV
                     DTAFYNLREDVKTLFGVDLQSDNKIQGVGSFMSWSFLFFKKQFIDSCLNDKKCILHLP
                     EFIFNDNKNLLALDTDTSDRIKAVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTD
                     EDLEYGYLTNTGNHYDVYLPPELFSQAYKLKNKEMNAQLDYLNRYAT"
     gene            917..2158
                     /gene="agp"
                     /locus_tag="ACHUQ7_003118"
     CDS             917..2158
                     /gene="agp"
                     /locus_tag="ACHUQ7_003118"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFR1136414.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGIVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPMITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRASAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(2196..2423)
                     /locus_tag="ACHUQ7_003119"
     CDS             complement(2196..2423)
                     /locus_tag="ACHUQ7_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFR1136415.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(2444..3040)
                     /gene="wrbA"
                     /locus_tag="ACHUQ7_003120"
     CDS             complement(2444..3040)
                     /gene="wrbA"
                     /locus_tag="ACHUQ7_003120"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFR1136416.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            3413..3520
                     /locus_tag="ACHUQ7_003121"
     CDS             3413..3520
                     /locus_tag="ACHUQ7_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KGG domain-containing protein"
                     /protein_id="HFR1136417.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            complement(3602..4930)
                     /gene="rutG"
                     /locus_tag="ACHUQ7_003122"
     CDS             complement(3602..4930)
                     /gene="rutG"
                     /locus_tag="ACHUQ7_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFR1136418.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSYAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNANLIMVAVTF
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(4951..5445)
                     /gene="rutF"
                     /locus_tag="ACHUQ7_003123"
     CDS             complement(4951..5445)
                     /gene="rutF"
                     /locus_tag="ACHUQ7_003123"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFR1136419.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            complement(5456..6046)
                     /locus_tag="ACHUQ7_003124"
     CDS             complement(5456..6046)
                     /locus_tag="ACHUQ7_003124"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFR1136420.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            complement(6056..6856)
                     /gene="rutD"
                     /locus_tag="ACHUQ7_003125"
     CDS             complement(6056..6856)
                     /gene="rutD"
                     /locus_tag="ACHUQ7_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFR1136421.1"
                     /translation="MKLSLSSPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTMLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            complement(6864..7250)
                     /gene="rutC"
                     /locus_tag="ACHUQ7_003126"
     CDS             complement(6864..7250)
                     /gene="rutC"
                     /locus_tag="ACHUQ7_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFR1136422.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFYIQCGLVKPDALVEIATIAHIAK"
     gene            complement(7262..7954)
                     /gene="rutB"
                     /locus_tag="ACHUQ7_003127"
     CDS             complement(7262..7954)
                     /gene="rutB"
                     /locus_tag="ACHUQ7_003127"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFR1136423.1"
                     /translation="MTTLTARPEAITFDPQQSAQIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEHFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            complement(7954..9045)
                     /gene="rutA"
                     /locus_tag="ACHUQ7_003128"
     CDS             complement(7954..9045)
                     /gene="rutA"
                     /locus_tag="ACHUQ7_003128"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HFR1136424.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIAQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSIPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQATEQTGRDVGSYVLFMVIADETDDATRAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLSALTQEVA"
     gene            9333..9971
                     /gene="rutR"
                     /locus_tag="ACHUQ7_003129"
     CDS             9333..9971
                     /gene="rutR"
                     /locus_tag="ACHUQ7_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFR1136425.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
BASE COUNT         2577 a         2644 c         2630 g         2357 t
ORIGIN      
        1 tgtctgttaa gagccatact ggttttaaaa gaacatgagt attcatgggc gctgggcagc
       61 cataagacgc aaaagcaagt atacgaagag tttattaaaa ttgttgataa acaaacgatc
      121 gaggctcttg ttgatacggc attctataat ctcagggaag atgtaaagac attatttggc
      181 gttgatctgc aatctgacaa caaaattcag ggtgtgggta gttttatgtc atggagcttt
      241 ctgtttttta aaaaacaatt cattgatagt tgcttgaatg acaaaaaatg catactgcat
      301 ttacccgagt ttatatttaa tgataacaag aatttgcttg ctttagatac cgacacgtcg
      361 gataggatta aagcggtgaa gaattttctt gctgttcttt cagatagcat ttgctcatta
      421 tttattgtta atagtaatgt ggcatcaatc tccttgggaa atgaatcctt ttcaacagat
      481 gaagatcttg agtatggata tttaacaaac actggcaatc attatgatgt ttacctccct
      541 cctgaacttt tttctcaggc ttataagtta aaaaataagg aaatgaatgc gcaacttgac
      601 tatttaaatc gttatgcaac ttaatggcaa aggcatatgc taaaaaccat tgttattagt
      661 ctcacacttt tttattggta aatattgtct ctgtattggt aacgccgcag atattctgtt
      721 tagccacagg tgcaattatc agcggcgtac gcgaggcagg ggctaatcag gcatagtttg
      781 cgtcaaacct tgcctgtttt tgaagatgta tatagaaaaa caggcgttca acaagccatt
      841 ttgcgaacct gttcccggaa aaaagtcata tttctgtcac actctttaat gattgataac
      901 aaaagaggtg ccaggaatga acaaaacgct aatcgccgca actgtggcag ggatagtttt
      961 actcgcttca aatgctcagg cacaaaccgt accggaaggc tatcagctac agcaagtgct
     1021 catgatgagc cgccataact tacgtgcgcc gctggcgaac aatggcagtg tgctggagca
     1081 gtcgacgccg aataaatggc ccgaatggga cgtccccggt gggcaactca ccaccaaagg
     1141 tggcgtgctc gaagtgtata tgggccatta catgcgtgaa tggctggcag agcaggggat
     1201 agtgaaatcg ggggaatgcc cgccgccgga caccgtttat gcctatgcca atagtctgca
     1261 acgtaccgtc gctaccgccc agttctttat tactggtgca ttccctgggt gtgatattcc
     1321 tgtgcatcac caggaaaaaa tgggcactat ggacccaacc tttaacccga tgatcaccga
     1381 tgattccgcc gcattcagtg aacaggcggt ggcggcaatg gagaaagagc tcagcaaact
     1441 ccagcttacc gacagctacc agctactgga aaaaatcgtt aactataaag attcccctgc
     1501 ctgtaaagag aaacaacagt gttcgctggt ggatggcaaa aataccttta gcgccaagta
     1561 tcaacaagaa ccaggtgttt ccgggccgct gaaagtcggc aactcgctgg tagatgcgtt
     1621 tactttgcaa tactacgaag gttttccgat ggatcaggtg gcctggggag aaatcaaatc
     1681 tgaccagcag tggaaggtat tgtcgaagct gaaaaacggt taccaggaca gtctgtttac
     1741 ctcaccggaa gtggcgcgca atgttgcgaa accgctggtc agttatatcg acaaagccct
     1801 ggtcaccgat cgcgccagtg ctccgaaaat cacggtgtta gttgggcacg actccaacat
     1861 agcctctctg ttaacggcgc tggatttcaa accgtatcag ttgcatgacc agaacgaacg
     1921 cacgccgatt ggcggcaaaa tcgttttcca gcgttggcat gacagcaaag ccaatcgcga
     1981 tttgatgaaa attgaatatg tgtaccagag tgcggaacag ttacgtaatg ccgatgcgtt
     2041 aaccctgcag gcacctgcgc agcgcgtgac gctggaatta agcggttgcc cgatagatgc
     2101 caacggtttc tgcccgatgg ataagtttga tagcgtgttg aatgaggcgg tgaaataaca
     2161 gaaaactccc ccgcgagaag cgggggagtc gctggttaaa cgtttttacg ttcgatggtc
     2221 tgttcgcccc aaaaaagcga atctttatcg gtcttagcaa aggctttgac taacacttca
     2281 tcactacctt cttcccaaat cttttctgcc attttttcgt cgtacccggc gacttcgaaa
     2341 atggcctcgg ctatttccgg cgacgtattg cgcagagatg cccattcacc gacgtgatga
     2401 gctttcgctt cttgagttgg catgcgtatc ctcctgttga agattagccg ttaagtttaa
     2461 ctgccagacc tgcgacatat tccccttgat aacgagcaat agacagttct tcctggcttg
     2521 gctggcgtga gccgtcaccg cctgcgatgg tggttgcgcc gtacggcgta ccgccgcgaa
     2581 cctgtgaaac gtcaaataat tcctgcgctg cgtagccaat ggggacaatc accatgccgt
     2641 gatgcgcaag ggtcgtccag gtggatgtga tggtttgttc ctgaccgccg ccagtaccgg
     2701 tggaactaaa gacgctcgcc agttttccgt atagtgcgcc ggaagcccac aggccgcccg
     2761 tctggtcgag gaaggtacgc atttgaccgg acatgttgcc aaagcgggta ggtgtaccaa
     2821 aaataatggc gtcgtaatcg gccagttctt gcggggttgc aaccggtgca gtttgcgttt
     2881 taccgcctgc tttttcaaat aattgcggcg gcatggtttc cggtacacgc ttaacgacaa
     2941 cttctgcgcc atccactttg cttgcaccct cagcgactgc gcgtgccatc gtttcaatat
     3001 gtccgtacat ggaataataa agcaccagaa ctttagccat ttctaaccac tcctcgtgtt
     3061 atctctattc cgtagcgatt cgctaccact tatttaaaga taagacgtcc ttttcagagt
     3121 gcaaatttca caaccactta tttgatttat aacaactttc acaagcacgt aattttgtcg
     3181 caaaatgaca catttttatc tcatcgcgtt tttttaatca taagagcggc ttatggataa
     3241 ttattggaga tgatatctat tctcgctaag aagctgttgc aggatattac caaacgcggg
     3301 tctgtccgcg tcagttcact aagcttagtc ccacgtagcg aaaatatggc agccgccata
     3361 cgccgcgtta attctatgca atatgatgtc tatacccaga cggaggtcag taatggcaaa
     3421 ccatcgaggc ggttccggca attttgcaga agaccgcgaa agagcatcag aagcaggtaa
     3481 aaaaggtggc aagagcagtc acggcaaaag cgacaactag ccgggctaat caatgacgaa
     3541 tgcatttttt tcttaagctc gtcaaaaagt catcaccgcc ggtaacccgg cggttttctg
     3601 tttatggctc ctgatgaaca acttctggcg gtggaacgtc aaccaacctt ctgcttaata
     3661 acgcattgag taaaatcgcg ccaaaggttg ctgtaccgat ccctcccaac gtaaaaccgc
     3721 caagcgtgag agcaaaatca cccgcgccca gcacgaaggt tactgcgacc ataatcaaat
     3781 tagcgttctg gcttaaatcg acacggtttt gtacccatat ccttgcgcct gcgacggcaa
     3841 tcagcccgaa cacaacaatt gatgcaccac caatcaccgc ggccggaatg gtatggatca
     3901 gcgcgccaaa tttcggcgaa aagcccaaca gcatagcgat gacggcagct gcaacaaaca
     3961 ccagcgtcga gtagactttt gtcacggcca tcacaccgat attttcagca taggtggtga
     4021 cgccgctacc gccgacagag ccggaaagca tcgttgccag accatcaccg acgaatgctc
     4081 gccccatata cgggtccata ttgcgtccgg tcatcccggc gaccgccttg agatgaccta
     4141 agttttccgc caccagaatc accgccacgg gcgcaatcaa catcatcgcc tggccattaa
     4201 aaccaggagt ggaaaaatgc ggcagaccga accaggcagc atagctgacg agagtaaaat
     4261 cgacggcttt tcccagccct aaaacgttgg tcatcacgcc atacagcaga caggcgacaa
     4321 ttaatcctac gagaatcaat aaccgctgga tcatgccacg ggtaaacacc gccaccagcc
     4381 caatacacag caccgtcatt accgccatcc agctatcaaa ggccgaagcc gatacacttt
     4441 tcactgcgat aggcgctaag ttcaggccaa tcgccatcac caccgcaccc gtcaccaccg
     4501 gcggcattag tcgttcaatc cagcgtgtac caattttcat caccaccagg ccaatgacgg
     4561 tataaaccag cccacaggcg ataatcccgc ccagcgcgat gctgatattc gggttaatgc
     4621 cctgaccgtt aaagcccgtc gcggcaatca ccacgccgac aaaagccgcg ctggagccga
     4681 gataactggg gacgcgcccg ccggtaataa agaaaaacag taacgtgccg atccccgaca
     4741 ttaaaatgga aagattggga tccagcccca tcagaatcgg cattaacacc gtcgcgccaa
     4801 acatcgccac cgcgtgttga acgcccatta ctgccgtctg agcaaacggc aatcgttcat
     4861 ccggcgcgac cacgccgctc tctgtagagg tcgattttaa ctgccagtga ggaaaaccga
     4921 acattgccat cagctgtctc cttaaggagg ttaacaagca gggcgcatca gcgcgtgata
     4981 actgcgatcg aaccacacca gcccgtaggg tgtggtgtga cgatgaatcg cttcgatggc
     5041 gcaaaacaga atgtcgtggg tgccgacgct caccacctgg ctgatacggc agtcaaacga
     5101 aaccagagcc tcttccagtt gcgggcatcc ggtcacaccc gtctgccagc gggcggcggc
     5161 aaagcggtgt tccatgggcg ttttgccgcc aaaaagattt gaaagcggct cctgcccggc
     5221 gctcagtgta tttacacaca gcgttcgatt ttcattgaat accggccaga cggacgcccc
     5281 acgattcagg cacaccagta atgtgggcgg cgtgtcggtc acgctgcaga cggcgctggc
     5341 ggtgaacccg gcgcgcccgg ctggaccgtc cgtggtgatg atattgaccg ccgcgcccat
     5401 acaggacatc gcatcgcgaa aggtttgttg atcaactatg ttcatagttt gctccttaca
     5461 acagcccgca ggcttcttca aaggacagac gtggcaggcg cgcaaaaagt ttgctgctat
     5521 cgccatagcc gatattaatc agcagattgc tcttcagcgt gctgcccgca aaaaaggcgt
     5581 cgtcaacgtg ttgacggtca aaacccgaca tcgggccggt atccagtccc agcgcacggc
     5641 aagcgacgat cagataagcc gcctgcatgg aactgttgcg aaacgctgtt tcttcggcaa
     5701 gttgtgggct ggaggtaaac caactgcggg catcaccgtg gggaaacagt agtggtaacc
     5761 gttcataaaa ttcactgtcc caggcgacga tagcggtgac gggcgcggtc agggtttttt
     5821 gcagattgcc gctggaaagc gccgggcgca gacgttcttt tccttctgca gtgcggataa
     5881 acacgatccg tgccggagaa cagttagctg atgtcggccc ccatttcatc agggcataaa
     5941 gctcctgtaa cgtctcatcg ctgacgggtg tctcccgcca gccgttgtga gtgcgggcat
     6001 cggtgaacag ggtgctaagc gcacctgggc taacggcttc gttcatagca attccttaca
     6061 gggcggcttc acggtgatgt aacaggctgg caagcccgtt gagtaacaga gcattaaacg
     6121 tttcgggatc ggtcacgttg caggcgtgtc cgccataggg catcaccatt ttctggctat
     6181 cgggcagggc ggcatgaagt tcactggaac atgctgttgg caccagcaga tcatcactgg
     6241 cgcagatgat ttgcaccggg cagcggatgc gatccgcatg gtgactaaag tcagcgcgtt
     6301 tgagggcgtt aagtcgacgt agtaaattat ttttgccctg aaaatgcgcc agtgccagcg
     6361 cgtcttctgc ctccaggcga ggtgcgcggg ccgccatcca gtcggcggga tagaggaaca
     6421 acggctgcgc ttccacccat gcttgcgcgc cgccgctata cagtaatcgt tcgcgaacct
     6481 gaaaacagcg gcgcgtatgg gcgtttattc gtagccagcc gttaacgctg accagcatag
     6541 ttaccgacgc gggataatcc agcgccagct gcatccccac cagcgcaccg agcgcatggc
     6601 cgaccaccgc gtaacgctca atccctgcgg ctaccagcgc ctgatgcagt tccgctgcca
     6661 tctgggcgat actgtaatct tctgccagtg tgtcgggatt attgccggtg ccgcgctggt
     6721 cgtaacagac tacctgatac tcctgctcca gcaccgccag ttgcggtaac cagtaactgc
     6781 cgctaccgcc aagacccgaa atcaacacca ctacgggtgc atcagcataa gggggagatg
     6841 agagtgaaag tttcatcgcg gcctcacttg gcgatatgcg caattgtggc gatttccacc
     6901 agcgcgtcag gttttaccag tccgcactga atgtagaatc gcgccggttt atcacccgga
     6961 aaaaactcgg cgtagatttc gttaatcgcg gcgtaatttt tccagtcggt aataaagatg
     7021 ctgttgaagg tcacatccgc catcgtgcca cccgccgtct cgatcacctt gcggatagtt
     7081 tccagaacgt ggcgggtttg cgcctttggg tcatcggcaa acagcacgtt attatgttga
     7141 tcaaaagcca gcgtaccgga gacatacacc acgccatcag ccagcgtgcc gggaacgaag
     7201 ggggccagcg gtgcgctgct gccagcggga ataattacgg attttggcat cgctaaactc
     7261 cttaagcgat acgagcaaag gacgtgggag aaagcgcgtc gcagaacgtt tcgacgtcgc
     7321 tgacccagcc aaaaaaggtt tcgatattga acaacgcggc tttctgtgca aattccggcc
     7381 ccgcctggtg tgttgcgtct tccagcacca cgccgaaatg ctccagaaaa aagccatcgc
     7441 gtagcgtcga ttcgacgcag acgttggtag cgataccggt gaaaaccaga tggcgtattc
     7501 cacggctgcg taaaatgctg tccagcggcg tattgaagaa accgctgtag cgcggcttcg
     7561 gcagcacaat atcgccaggc tgcggcacca gttcatccac cagttgataa tcccaggagc
     7621 ctttcgccag caatttcccc tgcagctgcg gctgcttacg catggttttc agggcgttcg
     7681 atttatgaaa attcggtgag ccggggccgc cagcctcgac atactgttca tcccagccat
     7741 tttgaaacca gatgatcaac atccctgccg ctcgcgctgc ggtcacagcg gtttgaatgt
     7801 tggcaatgac cgggcgagtg gttgagacat caaacccggc gagatctaag tagccgcctg
     7861 gcgtggcata agcgttttgc atatccacca cgatctgcgc actttgctgc ggatcgaagg
     7921 taatggcttc cggtcgagcg gttaaggttg tcatcatgcc acctcctgag tcagcgcaga
     7981 gagatgggcg cggcactgca tcagtggttg aatgcgctcg ccgaaggttt cgattcccga
     8041 cagaaaatca tcgaaggtca acagcacgcc ttctgcacca ggcacgcttg cgacttcatc
     8101 taacatgcgc gcgacactgg cgtaagaacc gactaacgtc cccatattga tgtttaccgc
     8161 cgaagtggga tcggccatct gacgaacgtt ggtgtcagta ccggagcggg tatctttctg
     8221 actttgttcg gttagccagc ttaacgcctc ttcatccgcg cccgctttgt agtgttccca
     8281 tttggcgcga gtggcatcgt cggtttcatc ggcaatcacc ataaacaaca cataagagcc
     8341 aacgtcgcgt ccggtttgct ctgtggcctg tttcatccgc gcagcggtcg gggcgaaagc
     8401 cgtgggtgta tttacgcctt tgccgaaaca gaagttgaaa tcggcatact gggcggagaa
     8461 cgccatgcca gcgtcgcttt gcccggcgca gatcactttc atggggatac tcggttgcgg
     8521 actgacgcga caatcattca tggtgaaaaa atcgccttta aaatcgcttt tccccgtgcc
     8581 ccacaggtcg cgcagcacct gaacatattc ggtgagatag tcgtaacgac gggagaaata
     8641 gtcatcgcca ggccagatac ccatctgctc atactcgggc ttttgccagc cagtcacgag
     8701 gttgacgcca aaacgcccgc cagagatgga gtcgatggtt gcggccatac gggcgacgat
     8761 tgccggaggt aacgttaagg tggcagcagt ggcgtaaatc tgaatgcgcg aggtcacggc
     8821 tgccagaccc gccatcaagg tgaacgactc aaggttgtga tcccagaact cagttttgcc
     8881 gccaaagcca cgcagtttga tcatcgacag ggcgaaatcg aaatggtaat gctccgcttt
     8941 ttgcgcaatg gctttattca gttcaaaggt cggcatgtac tgcggcgcgt gggtcgaaat
     9001 gagccagccg ttgttgccaa taggtacgaa tacgccaatt ttcatcatca acctctcttc
     9061 gtctcgtaaa gtgaaagtca gacggggcgc tgcatcctgc atatcctttt cagccgcgta
     9121 ttggcttgtt cgcaaagcgg atgccagttt ttaaaaagtg aatattatta atgtgttagc
     9181 attgtgttat ctaaaatatc tggtaaaaag tggactaaac ggtcaaaaca gttgcacata
     9241 aaacatgcat ccgtgcgcga tgagagtgca gaaggtcgag gccgggcggg ggttttgcta
     9301 tcctgttacc aatctacaag aggggagagc gcatgacgca aggcgcagtg aaaacaacgg
     9361 gtaaacgttc gcgcgcagta agcgcgaaga aaaaagcgat tcttagcgca gcactggaca
     9421 ctttttcaca attcggtttt cacggcacaa ggctggagca gatcgcagag ttggcgggtg
     9481 tttcaaaaac caatctgctg tattacttcc cgtcaaaaga ggcgctgtat attgccgtgc
     9541 tgcggcagat tctcgatatc tggctggcac cgttaaaagc gtttcgtgaa gatttcgccc
     9601 cgctggcggc gataaaagag tacatccgtc tgaagctgga agtctcacgc gattatccgc
     9661 aggcttcgcg cctgttctgt atggagatgc tggcaggcgc gccgttgtta atggatgaac
     9721 tgacgggcga tttgaaggca ttaattgatg agaaatcggc gctgattgcc ggttgggtca
     9781 aaagcggcaa actcgcgccg attgatccgc agcatttgat ttttatgatt tgggcttcca
     9841 ctcaacatta cgccgatttc gcccctcagg tggaggcggt gacaggcgcg acgttgcgcg
     9901 atgaggtatt tttcaatcaa acggttgaaa acgtgcagcg gattattatt gaggggattc
     9961 gaccacgtta aagatgacgg aggaggttgt aacatcctcc ggctacctgt ttaaccgatc
    10021 gtcattaaac tggcgttacc gccagcggca gcggtattca cactcagcga acgttcatcg
    10081 ataggaatta aaaccccaaa aagattaaaa aaacaccaca aaacggatgt ttcttcaaca
    10141 ccacttttgc tccatatgaa cggaaccgac gattaaactg gatggctctg attgattcag
    10201 ggtatgaa
//
LOCUS       DAZPCS010000132        10094 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZPCS010000132 DAZPCS010000000
VERSION     DAZPCS010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10094
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.132"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            333..1199
                     /gene="folD"
                     /locus_tag="ACHUQ7_003130"
     CDS             333..1199
                     /gene="folD"
                     /locus_tag="ACHUQ7_003130"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFR1136426.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            1201..1413
                     /gene="ybcJ"
                     /locus_tag="ACHUQ7_003131"
     CDS             1201..1413
                     /gene="ybcJ"
                     /locus_tag="ACHUQ7_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFR1136427.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1521..2030
                     /locus_tag="ACHUQ7_003132"
     CDS             1521..2030
                     /locus_tag="ACHUQ7_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR1136428.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKGLLFAGIILAMLPDADVLSFKFG
                     VAYGNVFGHRGFTHSLVLAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSLLDSV
                     TTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLPGMLL
                     MGMLWWRRR"
     gene            complement(2066..3451)
                     /gene="cysS"
                     /locus_tag="ACHUQ7_003133"
     CDS             complement(2066..3451)
                     /gene="cysS"
                     /locus_tag="ACHUQ7_003133"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFR1136429.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNSDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNRMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3625..4119
                     /gene="ppiB"
                     /locus_tag="ACHUQ7_003134"
     CDS             3625..4119
                     /gene="ppiB"
                     /locus_tag="ACHUQ7_003134"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFR1136430.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            4122..4844
                     /gene="lpxH"
                     /locus_tag="ACHUQ7_003135"
     CDS             4122..4844
                     /gene="lpxH"
                     /locus_tag="ACHUQ7_003135"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFR1136431.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4962..5471
                     /gene="purE"
                     /locus_tag="ACHUQ7_003136"
     CDS             4962..5471
                     /gene="purE"
                     /locus_tag="ACHUQ7_003136"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFR1136432.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNGWRKAQTDEVL
                     ENPDPRGAA"
     gene            5468..6535
                     /gene="purK"
                     /locus_tag="ACHUQ7_003137"
     CDS             5468..6535
                     /gene="purK"
                     /locus_tag="ACHUQ7_003137"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFR1136433.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(6672..7565)
                     /locus_tag="ACHUQ7_003138"
     CDS             complement(6672..7565)
                     /locus_tag="ACHUQ7_003138"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFR1136434.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(7562..8209)
                     /locus_tag="ACHUQ7_003139"
                     /pseudo
     CDS             complement(7562..8209)
                     /locus_tag="ACHUQ7_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            8212..9444
                     /gene="allC"
                     /locus_tag="ACHUQ7_003140"
     CDS             8212..9444
                     /gene="allC"
                     /locus_tag="ACHUQ7_003140"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HFR1136435.1"
                     /translation="MTHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFKK
                     RMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLA
                     IDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSF
                     ADAMKACGFTLPNAPLTPRQAIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTVT
                     LNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNVV
                     PGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKELV
                     ATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITDL
                     AEGVKTLALMLYQLAWQE"
     gene            9455..>10094
                     /gene="allE"
                     /locus_tag="ACHUQ7_003141"
     CDS             9455..>10094
                     /gene="allE"
                     /locus_tag="ACHUQ7_003141"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HFR1136436.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLIKNIIPDFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLFSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVY"
BASE COUNT         2338 a         2713 c         2678 g         2365 t
ORIGIN      
        1 cgttagatga atacttccgc gcaaaatata ttcaccacaa atatagacct gaagcggtaa
       61 attaccaggc tgaaaattct ttttatattg tcaggtattt cttaaattat cttaatcctt
      121 aaacaaggaa ataaatcagt tccagattta caacgccatc atgtacgaaa aatgaagctt
      181 tcaatctcag cgacctcagc gacggtgcgc ctaaccttcg caagaggtcg cttcacgcga
      241 taaatctgaa acgaaacctg acagcgcgcc ccgcttctga caaaatagac gcatcccctt
      301 cgacctacgt aacagatgga atcctctctc tgatggcagc aaagattatt gacggtaaaa
      361 cgattgcgca gcaggtgcgc tctgaagttg ctcaaaaagt tcaggctcgt attgcagccg
      421 gactgcgggc accaggactg gccgttgtgc tggtgggtag taaccctgca tcgcaaattt
      481 atgtcgcaag caaacgcaag gcttgtgaag aagtcgggtt cgtctcccgc tcttatgacc
      541 tcccggaaac caccagcgaa gcggagctgc tggagcttat cgatgcgctg aatgccgaca
      601 acaccatcga tggcattctg gttcaactgc cgttaccggc gggtattgat aacgtcaaag
      661 tgctggaacg tattcatccg gacaaagacg tggacggttt ccatccttac aacgtcggtc
      721 gtctgtgcca gcgcgcgccg cgtctgcgtc cctgcacccc gcgcggtatc gtcacgctgc
      781 ttgagcgtta caacattgat accttcggcc tcaacgccgt ggtgattggc gcatcgaata
      841 tcgtcggtcg cccaatgagc atggaactgt tgctcgcagg ttgcaccacc accgtgactc
      901 accgcttcac taaaaatctg cgtcatcacg tagaaaatgc cgatctgttg atcgttgccg
      961 ttggcaagcc aggctttatt cccggtgact ggatcaaaga aggcgcaatt gtgattgatg
     1021 tcggcatcaa ccgtctggaa aatggcaaag ttgtgggcga cgtcgtgttt gaagacgcgg
     1081 ctaaacgcgc ctcatacatt acgcctgttc ccggcggcgt tggcccgatg acggttgcca
     1141 cgctgattga aaacacgcta caggcgtgcg ttgaatatca tgatccacag ggtgagtaac
     1201 atggcgacat tttctttagg taaacatccg cacgttgagc tgtgcgactt gctgaaactg
     1261 gaaggctgga gcgaaagcgg cgcgcaggcg aaaatcgcga ttgccgaagg ccaggtgaaa
     1321 gtcgacggtg cggttgaaac gcgcaaacgc tgcaaaatcg tcgccggtca gacagtgagt
     1381 tttgcaggac acagcgtaca ggttgttgcc tgatcccttt cccggcctgt atcaacaggc
     1441 cggttctctt ttcagacttt tgccctctac gccccgttca tccatgctga tagcatcgac
     1501 attttgccga agcgaccatc atgccaaccg ttattacgca cgccgccgtt cccctttgca
     1561 ttggtttagg gttgggttcg aaaggtctgt tatttgccgg aatcatcctg gcgatgctgc
     1621 ccgatgccga cgtattgtcg tttaaatttg gcgttgctta cggcaatgtt tttggtcatc
     1681 gcggttttac ccattcgctg gtgcttgcgt ttgttgtccc gctcttatgt gtgctgattg
     1741 gacgtcgatg gttcagggct gggctgattc gctgctggct gtttttaacc gtctcgttgc
     1801 tatcgcacag cttactggat tcggtaacca ctggcggtaa gggcgttggc tggctgtggc
     1861 cgtggtcaga tgaacgcttt ttcgctccct ggcaggtgat taaagtcgcg ccgtttgcat
     1921 tgtctcgtta caccacagcg tacgggcatc aggtgattat ttccgaattg atgtgggtgt
     1981 ggttgccggg gatgctactg atgggaatgt tgtggtggcg cagacgataa ccggatgcga
     2041 aaactcgcat ccggcaattg cgcaattact tacgacgcca ggtggtccct tgcgggccat
     2101 cttccagcac gatccccatc tcgttaagac ggtcacgtgc cgcatctgcc gccgcccagt
     2161 ctttcgcttt acgggcatcc agacgctgtt gaattaacgc ttcaatctca gccacttcgc
     2221 tgtcgtctgc ctgcgcgccg ctttgcagga acgcttccgg ttcttgctcc agcaggccca
     2281 atacagcgga aagtttacgc aggtgagatg ccattgcatt cgccgctgcc atatcttctg
     2341 ctttcagacg gttcacttca cgcgccatat caaacagcac ggaataggct tccggggtgt
     2401 tgaaatcgtc gtccatcgct tcaataaagc gcgcttcaaa cgcttcgcca ccggcaggcg
     2461 caacggtttt atctgtgccg cgcagcgcag tgtagaggcg ctccagcgcc gcacgcgcct
     2521 gcttcaggtt ctcttcgctg tagttcagct ggctgcgata gtggcccgac atcaggaagt
     2581 aacgcacggt ttccgcgtcg tagtatttca gcacatcgcg cacggtaaag aagttaccca
     2641 gcgatttgga catcttctcg cggtcaacca tcaccattcc ggagtgcatc cagtagttca
     2701 catactgacc atcatgggca caggtggact gcgcgatttc gttctcgtgg tgcgggaaca
     2761 tcaggtctga accgccgccg tggatatcaa agtggttacc cagctgcttg cagttcatgg
     2821 ccgaacattc aatgtgccag ccaggacgac ccgcgcccca cggagacggc cagctcggtt
     2881 cgccctcttt cgacatcttc cacagaacga agtccatccg gttgcgtttg tcatcaacca
     2941 cgtcaacgcg cgcgccagcc tgcagctggt cgagatcctg acgcgacagc acgccataag
     3001 ttggatcggt cgggacgtcg aacatcacgt cgctgttgtc cgccacataa gcgtgacctt
     3061 tggcgatcag ttgttcagtg agttcaataa tttctgcgat atggtgcgtc gcgcgcggct
     3121 ccatatccgg gcgcagaatg ttcaaagcat caaaatcttt gtgcatttcg gcgatcatgc
     3181 gatccaccag cgccacaaag ctttcgccat tttcattggc gcgtttgatg attttgtcgt
     3241 cgatatcggt aatgttgcgc acatacttca gtttatagcc gaggaaacgc agatagcgcg
     3301 caaccacgtc aaaagcaaca aaggtacgcc cgtgaccgat atgacagaga tcgtaaacgg
     3361 tgattccaca cacgtacatg ccgacttccc cggcgtgaat aggcttaaat tcctcttttt
     3421 ggcgtgtcag agtattgaag atttttagca tcgaagattc cgtttagaca tgtgtgggta
     3481 attgagttgc gtataatacc catatttccc gcgcgaatca gcatacattg cgtgatgatc
     3541 cgatcgtgcg gttatgctat aacaccaccc tatatatgac ccgaactggg ttgaagcacc
     3601 aatcaaacgg aacaggatgc aaaaatggtt actttccaca ccaatcacgg cgatattgtc
     3661 atcaaaactt ttgacgataa agcacctgaa acagttaaaa acttcctgga ctactgccgc
     3721 gaaggttttt acaacaacac cattttccac cgtgttatca acggctttat gattcagggc
     3781 ggcggttttg aaccgggcat gaaacaaaaa gccaccaaag aaccgatcaa aaacgaagcc
     3841 aacaacggcc tgaaaaatac ccgtggtacg ctggcaatgg cacgtactca ggctccgcac
     3901 tctgcaactg cacagttctt catcaacgtg gttgataacg acttcctgaa cttctctggc
     3961 gaaagcctgc aaggttgggg ctactgcgtg tttgctgaag tggttgacgg catggacgtg
     4021 gtagacaaaa tcaaaggtgt tgcaaccggt cgtaacggta tgcaccagga cgtgccaaaa
     4081 gaagacgtta tcattgaaag cgtgaccgtt agcgagtaat cgtggcgaca ctctttattg
     4141 cagatcttca tctctgcgtg gaagaaccgg cgatcaccgc cggttttctg cgttttttag
     4201 cgggggaagc ccgcaaggcc gacgcgctgt atattcttgg cgatctgttt gaagcatgga
     4261 ttggcgacga cgatcccaac ccactccatc gccagatggc ggcagcgatc aaagcggtgt
     4321 ccgattccgg cgttccctgt tatttcattc atggcaaccg tgattttctg ctcggcaaac
     4381 gctttgcccg tgaaagcggc atgacattat tgccggaaga aaaggtgctc gaactttatg
     4441 gtcgccgggt gttgattatg catggcgaca cgctgtgcac cgatgacgcg ggttatcagg
     4501 cttttcgcgc caaagtccac aaaccctggc tgcaaatgct attcctcgcc ctgccgttgt
     4561 ttgtgcgcaa acgcattgcc gcgcgaatgc gcgcgaacag caaagaagcc aacagcagta
     4621 aatcgctggc gatcatggac gttaaccaaa acgctgtggt cagtgcgatg gaaaaacatc
     4681 aggtgcaatg gctaatccac gggcataccc atcgcccggc ggtgcatgaa cttatcgcca
     4741 accagcaacc tgcttttcgc gtggtactgg gtgcctggca tacggaaggt tcgatggtga
     4801 aagtcacggc tgatgacgtt gagctgattc attttccgtt ttaaaaaacc cacaactttg
     4861 ctgatttcac agccacgcaa ccgttttcct tgctctcttt ccgtgctatt ctctgtgccc
     4921 tctaaagccg agagttgtgc accacaggag ttttaagacg catgtcttcc cgcaataatc
     4981 cggcgcgtgt cgccatcgtg atggggtcca aaagcgactg ggctaccatg cagttcgccg
     5041 tcgaaatctt cgaaatcctg aatgtcccgc accacgttga agtggtttct gctcaccgca
     5101 cccccgataa actgttcagc ttcgccgaaa gcgccgaaga gaacggttat caggtgatta
     5161 ttgcgggcgc aggcggcgca gcgcacctgc caggcatgat tgccgccaaa acgctggtgc
     5221 cggtgctggg cgtgccagta cagagcgccg cactgagcgg tgtcgatagc ctctactcca
     5281 tcgtacaaat gccgcgcggc attccggtgg gtacgctggc gattggtaaa gctggcgcgg
     5341 caaacgcggc attactggca gcacaaattc tcgcgactca cgataaagag ctacaccagc
     5401 gtctgaatgg ctggcgcaaa gcccagaccg acgaagtgct ggaaaacccg gacccgcgag
     5461 gtgcggcatg aaacaggttt gcgtcctcgg taacgggcag ttaggccgta tgctgcgtca
     5521 ggcaggcgaa ccgttaggca ttgctgtctg gccggtcggg ctggacgctg aaccggcggc
     5581 ggtgcctttt caacaaagcg tgattaccgc tgagatcgaa cgctggccgg aaaccgcatt
     5641 aacccgcgag ctggcgcgtc atccggcctt tgtgaaccgc gatgtgttcc cgattattgc
     5701 tgaccgtctg actcagaaac agcttttcga taagctccac ctgccgaccg cgccgtggca
     5761 gttacttgcc gatcgcagcg agtggcctgc ggtgtttgat cgtttaggtg aactggcgat
     5821 tgttaagcgt cgcactggtg gctatgacgg tcgcggtcaa tggagtttac gcgccaatga
     5881 aaccgaacag ttaccggcag agtgttacgg tgaatgtatt gtcgagcagg gcattaactt
     5941 ctctggtgaa gtatcgctgg ttggcgcgcg cggctttgat ggcagcaccg tgttttatcc
     6001 gctgacgcat aacctgcatc aggacggtat tttgcgcacc agcgtcgctt ttccgcaggc
     6061 caacgcacag cagcaggcgc aagccgaaga gatgctgtcg gcgattatgc aggagctggg
     6121 ctatgtgggc gtgatggcga tggagtgttt tgtcaccccg caaggtctgc tgatcaacga
     6181 actggctccg cgtgtgcata acagcggtca ctggacacaa aacggtgcca gcatcagcca
     6241 gtttgagctg catctgcggg cgattaccga tctgccgtta ccgcaaccag tagtgaataa
     6301 tccgtcggtg atgatcaatc tgattggtag cgatgtgaat tatgactggc tgaaattgcc
     6361 gctggtgcat ctgcactggt acgacaaaga agtccgtccg gggcgtaaag tggggcatct
     6421 gaatttgacc gacagcgaca catcgcgtct gactgcgacg ctggaagcct taatcccgct
     6481 gctgccgcca gagtatgcca gcggcgtgat ttgggcgcag agtaagttca gttaagcagg
     6541 tgctcatttg attgccggat gcggcgtaaa cgccttatcc ggcttaccgc tccggtaccc
     6601 attgtaggcc tgataagacg cgtcaagcgt cgcatcaggc atggtgcacc gattgccgga
     6661 tgctctcatc actacagcga aatacaggtc cccgcttcgc ccgccagcgt ctcttcaatt
     6721 cgcgataacg ccccaatcca cgcgggttta ctacggcttc tgacataacc actcaccgcc
     6781 gttaccttcg gccccatcga accatcggct ttggcaaatg gcgctaactc atccggtgtg
     6841 gcatggcgaa tggcacgttg ctgcggcgtt ccccagtttt catataccgc atcagcatcg
     6901 gtgaggatca ccagtccatc ggcattaatc tgctcggcga gcaacgcagc ggcgagatct
     6961 ttatcaatca ccgcttcact ccctgcccca tcttccgtca caggcacacc gccgccgcca
     7021 ctgcaaatca ccacatgccc ctctttgagc aacaactcga tggcttcgct gtcgagaatt
     7081 ttacgcggtt gcggagacgc caccacccgg cgcaaatatt taccatcacg tttcatctgc
     7141 cagccgtaag ccgcttccag tgcctcttgt tcttctggct gataaaccgg accaataaat
     7201 ttctctggct gcaaaaacgc cggatcatca ggcgaaacct caatgcgcgt cagcaccgtc
     7261 gtaacaggcg gcatctgcgg ctgtgcgctc aagctctgcg ccagcatata gccaatcatc
     7321 ccctggcttt ccgccaccag tacatccagc ggatacggtt ctacctcttt ccacgccaga
     7381 ttctgtaacg ccagcagccc cacctgcggc ccgttgccgt gaacaatcgc cagccgataa
     7441 gaacgggcca ggcgtgccag cgcgggtaca gcactggcga tattgcgata ttgattttcc
     7501 gccgtcagcg cctcaccgcg ctggagtaag gcgttgcccc caagagctac aaccagtgtt
     7561 ttcatggttt tccttgtaat aattgttggc caagccagaa ccccagcagc gtatctgccc
     7621 ctgacgtatg ccccagcgcc agcagcgaat caatcgcaac agcggtacgt ttcgggcaac
     7681 tcagagcatg gacaaagtgc aggagtggcg aggcgaaata tccttgcgcg gcataacgta
     7741 aataactgac gctcaccgct gtggtaacga gttgaagatt gtcggaacag gcaaaaaacg
     7801 ggcgaccggc gcgcgcatct aaagcgccat aataccaggc cgccagcagc attccgctca
     7861 acgtgtcatc atgacttggc gttaatccgg ggcctttacc cagccagtgc cgccagtcgg
     7921 tcttaacgcc attgagcgcg gcctgaaaac agtgacgaaa ctggcgtaat tcagcgggca
     7981 gcggatcgct tgccgccatc gccagtggcc cgaaaagtcc ggtttcctcc gcgcgttgca
     8041 tccatgcagc tgcaagcggt tgaggatgcg aaggcggcgt aatacgcagc aaacaataac
     8101 gctgtggctg tttaaccgtg aaacgcccga ggcgaatccc ttgagccaca acctgtggtc
     8161 gttcattgcc gcataatcca ccgcataacg catcgaattg cgtgcggcga aattacacat
     8221 tcccgtcaag ctatagaaga aacgctgccc tggctttcct cttttggcgc tgacccaacg
     8281 ggtgggatga cccgtttact ttattcgccg gaatggctgg aaacccagca gcaatttaaa
     8341 aaaagaatgg cagcaagtgg gctggaaaca cgtttcgatg aagtggggaa tttatacggt
     8401 cgcctgagtg gcaccgaata tccacaggaa gtggttctga gcggttcgca tatcgatacc
     8461 gtggttaacg gcggtaacct cgacgggcaa ttcggcgcgc tggcggcgtg gctggcaatt
     8521 gactggttga aaacgcaata cggcgcaccg ttacgtacgg tcgaagtggt ggcgatggca
     8581 gaagaagaag gcagccgctt cccgtatgtc ttctggggca gtaaaaatat ctttgggctg
     8641 gcgaatcctg acgacgtgcg gaatatctgt gatgccaaag gaaatagttt tgccgatgcg
     8701 atgaaggctt gcggatttac tctgccgaac gccccactaa ctccgcgtca ggctataaaa
     8761 gcctttgttg aactgcatat tgaacagggc tgtgtgctgg aaagtaatgg gcaatcaatt
     8821 ggcgtggtga atgcaattgt cgggcagcgt cgctatacgg tgacgctgaa cggcgaatca
     8881 aaccatgcag gcaccacgcc gatgggttat cgtcgtgata cggtttacgc tttcagtcgc
     8941 atttgccatc agtcgatcga aaaagcgaaa aagatgggcg atccgctggt tctgaccttt
     9001 ggcaaagtag agccgcgccc gaatacggta aatgtggtgc cgggtaaaac cacgttcacc
     9061 attgattgtc gtcataccga cgctgccgtg ctgcgcgatt tcacccaaca gttagaaaac
     9121 gacatgcggg cgatttgcga tgaaatggac attggtattg atatcgattt atggatggac
     9181 gaagaacccg tgccgatgaa taaggagctg gtcgccaccc tgacagaatt gtgtgaaaga
     9241 gaaaaactga attaccgggt gatgcacagt ggtgccgggc acgacgcgca aattttcgcg
     9301 cctcgcgtac caacctgcat gatttttatc cccagcatca acgggatcag ccataacccg
     9361 gcggaacgca ccaatattac tgaccttgcc gaaggggtca aaacgttggc actcatgctt
     9421 tatcaacttg cctggcagga ataaggagtc ataaatggga tatttaaata acgtcaccgg
     9481 ttaccgcgaa gatttactgg ctaaccgtgc gattgttaaa cacggtaatt tcgcactgtt
     9541 aaccccggat ggtctgataa aaaatattat tccggacttt gaaaattgtg acgcgacaat
     9601 cctctccacg ccaaagctgg gtgcctcttt tgttgattat ctggtcacac tgcatcaaaa
     9661 cggtggcaac caacagggct tcggtggcga aggcattgaa acgttcctgt atgtgatctc
     9721 tggaaatatc actgccaaag ccgaaggcaa aacatttgcc ttaagcgaag gtggctatct
     9781 ttattgccca ccaggctcct taatgacgtt tgttaacgcc caggccgaag acagccaaat
     9841 ctttttatat aagcgccgct atgttccggt agaaggccat gcaccgtggc tgttttctgg
     9901 caatgccagc gaactggaac gcattcacta tgaaggtatg gatgatgtga tcctgctgga
     9961 ttttctgccc aaagagttag gttttgatat gaacatgcat atcctctctt ttgcaccagg
    10021 tgccagccac ggttatatcg aaacacacgt tcaggaacat ggtgcctata ttctttccgg
    10081 tcagggggtt tata
//
LOCUS       DAZPCS010000133        10008 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZPCS010000133 DAZPCS010000000
VERSION     DAZPCS010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10008
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.133"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            73..1202
                     /gene="ompN"
                     /locus_tag="ACHUQ7_003142"
                     /pseudo
     CDS             73..1202
                     /gene="ompN"
                     /locus_tag="ACHUQ7_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            1343..1777
                     /gene="uspF"
                     /locus_tag="ACHUQ7_003143"
     CDS             1343..1777
                     /gene="uspF"
                     /locus_tag="ACHUQ7_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFR1136437.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            1953..2888
                     /gene="ttcA"
                     /locus_tag="ACHUQ7_003144"
     CDS             1953..2888
                     /gene="ttcA"
                     /locus_tag="ACHUQ7_003144"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFR1136438.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(3017..4384)
                     /gene="dbpA"
                     /locus_tag="ACHUQ7_003145"
     CDS             complement(3017..4384)
                     /gene="dbpA"
                     /locus_tag="ACHUQ7_003145"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFR1136439.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTARAG
                     NSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKKAK
                     MRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKTCR
                     VRLLK"
     gene            complement(4414..4587)
                     /gene="ynaL"
                     /locus_tag="ACHUQ7_003146"
     CDS             complement(4414..4587)
                     /gene="ynaL"
                     /locus_tag="ACHUQ7_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFR1136440.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(4862..5845)
                     /gene="zntB"
                     /locus_tag="ACHUQ7_003147"
     CDS             complement(4862..5845)
                     /gene="zntB"
                     /locus_tag="ACHUQ7_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFR1136441.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(5859..5906)
                     /gene="ydaN"
                     /locus_tag="ACHUQ7_003148"
     CDS             complement(5859..5906)
                     /gene="ydaN"
                     /locus_tag="ACHUQ7_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFR1136442.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            6100..7332
                     /gene="dgcM"
                     /locus_tag="ACHUQ7_003149"
                     /pseudo
     CDS             6100..7332
                     /gene="dgcM"
                     /locus_tag="ACHUQ7_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
     gene            complement(7353..7916)
                     /gene="smrA"
                     /locus_tag="ACHUQ7_003150"
                     /pseudo
     CDS             complement(7353..7916)
                     /gene="smrA"
                     /locus_tag="ACHUQ7_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
     gene            complement(8170..9624)
                     /locus_tag="ACHUQ7_003151"
     CDS             complement(8170..9624)
                     /locus_tag="ACHUQ7_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HFR1136443.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTMAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWLTDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGG
                     VGIFSTVYLIPVFLGQIRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMA
                     GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKERLKLA
                     SGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNR
                     SRSDQTSEILASTKLLSQLMLREAQTMAFSVPFC"
     gene            complement(9621..>10008)
                     /locus_tag="ACHUQ7_003152"
     CDS             complement(9621..>10008)
                     /locus_tag="ACHUQ7_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFR1136444.1"
                     /translation="APIDGIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQI
                     AGMKPGMKAEIRADILKGEVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVR
                     IAFDDAKELKQLLRPGLSVTVSVDER"
BASE COUNT         2759 a         2496 c         2271 g         2482 t
ORIGIN      
        1 ttctccttta ggatgttttc actccccgaa atggggataa aaggcaaata aaataactaa
       61 aggatttatt caatgaaaag aaaagtactg gcacttttaa ttcctgccct gctcgccgca
      121 ggtgctgcac atgcagccga agtttataat aaagacggca acaaattaga tctgtatggc
      181 aaagttgatg gcctgcatta tttttctgat aattcagcga aagatggcga ccagagctat
      241 gcgcgtctgg gttttaaagg cgaaacccaa attaacgatc aacttactgg ctacggtcaa
      301 tgggaataca atattcaggc aaacaacact gaatcttcaa aaaaccagtc atggacccgt
      361 ctggcatttg ccggactgaa atttgcagat tacggttctt tcgattacgg acgtaattat
      421 ggcgtaatgt acgacatcga aggctggacc gatatgctgc ctgaatttgg cggtgactct
      481 tataccaatg cagacaactt tatgactggt cgagccaatg gcgtcgcgac ttatcgtaat
      541 actgatttct tcggtctggt aaatggtctg aacttcgcgg tgcagtatca aggtaacaac
      601 gaaggagcca gtaatggtca ggaaggcacc aataacgaac gcgatgttcg tcatgaaaac
      661 ggtgacggct ggggtctttc cacaacatat gatttaggca tgggctttag cgctggtgcg
      721 gcatacacct cttctgaccg caccaatgac caggttaacc atactgcggc gggtggtgat
      781 aaagcagacg cgtggactgc tgggctaaaa tacgatgcta acaatattta cctagcaacc
      841 atgtattcag aaacgcgtaa tatgaccccg tttggcgaca gcgattatgc agttgcgaac
      901 aaaacccaga actttgaagt tactagtacc agtttgattt ttgtctgcgt ccggcagtct
      961 ctttcctgat gtctaaaggc cgtgaccttc acgctgctgg tggtgcagac aacccggcag
     1021 gtgttgatga taaagatctg gttaaatacg ccgatgttgg cgcgacttac tatttcaata
     1081 aaaacatgtc cacctatgtt gactataaaa tcaacctgtt ggatgaagat gacagtttct
     1141 acgctgccaa cggcatctct accgatgata ttgtcgcttt aggtctggtt tatcagttct
     1201 aaatcctcct gcccgctgtt atggcgggct ttttctgctt attcttcctc ctttgatcta
     1261 aattaaaaat gtgaactccg tcattacaca aaaagtgtca tctggcgtta cactttatgc
     1321 ggatactaaa acaggaggtt ttatgaacag aacgattctt gtccctatcg atatttcaga
     1381 ttcagaatta actcaacgcg tgattagcca cgttgaggca gaggcaaaga ttgatgatgc
     1441 agaggttcat ttcctgacgg taataccttc actgccctac tatgcctctc tgggtttagc
     1501 gtattccgca gaattaccgg caatggatga cctgaaagcg gaagccaaat cgcaactgga
     1561 agagatcatt aaaaaattta aactgccaac cgacagagtg catgtccatg ttgaggaagg
     1621 ctcgcccaaa gaccgcattc tggaattggc gaagaagatc cccgctcata tgatcatcat
     1681 tgcttcccat cgaccggata tcaccactta tctgctcggt tccaacgccg cagctgtagt
     1741 gcgtcacgca gagtgctccg tgctggttgt gcgctgacac taacgcccgc acattgctgc
     1801 gggctttttg attcatttcg caaatgtgct gacattttcc cctctaatcc gtaccataca
     1861 cgccacagtt tttatatcag atttcttatg ctgagcgttc cggcatgacg catactcttc
     1921 tgatgccata taacgaattg agtcgctttt aaatgtcgca aaatcaagaa attagtaaga
     1981 aagaacaata caacctgaac aaattacaaa aacgtctgcg tcgtaacgtg ggcgaagcca
     2041 ttgctgactt caatatgatt gaagaaggcg atcgcatcat ggtttgcctc tccgggggta
     2101 aagacagcta taccatgctg gagattctgc gcaatttgca gcaaagcgcg ccaatcaatt
     2161 tttcgctggt ggctgttaac ctcgatcaaa agcaaccggg cttcccggaa cacgttctgc
     2221 ccgagtatct tgaaaagctg ggcgttgagt acaagattgt tgaagagaac acttacggta
     2281 tcgtgaaaga gaagattcca gagggcaaaa ccacttgctc actgtgttct cggctacgtc
     2341 gcggtatcct ttatcgcacc gcaacggaac tggggacgac gaagatcgcg ttgggtcacc
     2401 atcgtgacga tatcctgcaa acgttgttct taaatatgtt ctacggcggc aagatgaaag
     2461 gtatgcctcc gaaactgatg agcgatgatg gcaaacatat cgttattcgt ccactggcct
     2521 actgccgcga gaaagatatt cagcgatttg ccgatgcaaa agcgttcccg attattccgt
     2581 gcaacctgtg cggttcacag cctaacctgc aacgtcaggt gattgctgac atgttgcgtg
     2641 actgggataa acgttatcca gggcgtatcg agacgatgtt cagcgcgatg cagaatgtgg
     2701 tgccgtcgca tctgtgcgat accaacctgt tcgatttcaa aggcattacc cacggttctg
     2761 aagtggttaa cgggggtgat ctggcgtttg atcgcgaaga gatcccacta caaccggcgg
     2821 gctggcagcc agaagaagat gaaaatcagt tggatgagtt acggctgaat gtggttgaag
     2881 tgaaataacc aggatagcgc ccgatgcgca agcgtatcgg gctactctta tggaggccgg
     2941 ataagacgcg gccagcgtcg catctggcaa tcccgaataa gatgtttact cttgcacccg
     3001 gcaattcaac atttcattat tttaataacc gcacccggca cgtttttcct ttaatcttcc
     3061 cgccctgtaa ctgtttccat gctttatgag caacagcctg acggaccgcg acatagacat
     3121 gcgccggatg cacggcgatt ttgccaatat ctgcgccatc aagcccgata tctcctgtca
     3181 gtgcacctaa tacatcaccc gggcgcattt tggctttttt cccgccatcg atacacaacg
     3241 ttgccatttc tgcttccagc gtcacaatgg aactattagc tggcggcgtt tgccagttaa
     3301 gttttatctg caacatgtca gaaatgatat tggcccgctg tgcttcttcc ggagcacaga
     3361 aactgatcgc cagaccgcta tttcctgcac gagctgtacg accgatgcga tgtacatgaa
     3421 cttcagggtc ccacgccagc tcaaagttca ccaccagctc aagcgattta atatccagac
     3481 cacgcgcagc aacatcagtc gcaaccagta cacgggcgct accgttagca aaacgtacca
     3541 gggtctgatc gcgttgctcc agatcgccgt gtaacgacaa tgcactttgc tctacttcat
     3601 tcagcgcgtc gcagacagcc tggcaatctt ttttggtatt gcaaaacacc acacaagagg
     3661 atggctgatg caagcttaat aaccgttgca acagaggaat tttgccttta ctggatgtct
     3721 cataaaattg ttgttcaatg ggtggcaaag catctgttga gtcaatttca atcgccaaag
     3781 gatcgcgttg cactcgtccg ctgattgcgg cgatggcttc cggccaggtt gccgaaaaca
     3841 gaagcgtctg tcgagatgca ggcgcaaaac ggatgacatc atcaatggca tcgctaaatc
     3901 ccatatccag catgcggtcg gcctcatcca tcaccaaagt attcagcgca tccagtgata
     3961 ccgtgccttt ttgcaagtga tccagcaaac gacccggcgt tgccacgata atatgcggcg
     4021 catgttgcag cgaatcacgc tgcataccga acggttgacc accgcacaac gtcaaaattt
     4081 tggtatttgg cagaaaacgc gccagccgac gcaattcacc tgccacctga tccgccagtt
     4141 cacgcgtagg acacagcact aaagcctggg tttgaaatag cgacgcatca atttgctgta
     4201 acaagccgag gccaaaagcc gccgttttgc cgctgccggt tttcgcctgc acgcgaacat
     4261 cttttccggc aaggatcgcc ggaagcgcgg cggcctgcac cggcgtcatg gttaaataac
     4321 ccaactcatt aaggttcgtg agttgggcgg gaggcaaaac attcagggta gaaaaagcgg
     4381 tcacaatcta ttctcgtggt catcgacgca aagttagcag gcgcgtatcc tcgcagatct
     4441 acgctcacga tgcgacaatt taatcggttc ttcatcgggt ggtgggtcag gcatgggttg
     4501 cgggcgaggg atcggatcgg gcactggaac aggatcgcca ggaatcggtt cagggacagg
     4561 aatttgcaaa taaataagtg tcgtcatatt tccctccggt cattgggtgg actcttaaag
     4621 ggtagacgct gataaataac aggcaaaaaa aagccgactc atcaaagtcg gcgtcgtacg
     4681 aatcaattgt gctatgcagt aattcaaaaa aggaagtaag acaatatgga gcgcaacgcc
     4741 catcgcttga cgttgcattc acctgcaagg gagatattgc cctgaatggg tagagagttt
     4801 attgacttcg ctcaaacttt gcggcgtttt tgtatacaga cagccggaaa aattgctttt
     4861 gttacaacca tttactacgg tgcaaccata aagcaacacc accaataaga acaactaaca
     4921 gaatacaaaa aattgaaaat ccgaattgcc acccgccgcc agggatccca ccaaggttga
     4981 cgccaaataa cccggtcaga aaggtactgg gtaaaaagac cattgccatc aacgacattg
     5041 tataggtacg acgagctaaa ttttcctgca tcacctgagc gatttcatcc gccatcacgc
     5101 cagtccgtgc tatacaggcg tcgatttcgt caaggccgcg cccaaggcga tcggcaatat
     5161 cctgcatccg acggcgttgg tcatcgctca tccacggcaa acgttcactg gcaagacgag
     5221 cataaacatc acgttgcggt gccatatagc gacgcatcac aattaattgt ttgcgcagca
     5281 gagccaggaa tccacgcggt ggaatttgct gatcaaggag attatcttca aggtcgataa
     5341 ttttatcgtg cagctgctcg ataaattcac tggaatgatc ggtcaacgca tcgcacacat
     5401 ccaccagcca tcccccgcaa tcggtcggac ccgtgccctc ttccagatcg ctcaccacat
     5461 cgtccagcgc cagcactttg cgttgtcggg tcgaaacaat taaccgcccg tccatatata
     5521 cacgcatggc gaccagttga tcggggcgtt catcggtgct gccgtttata cagcgcaatg
     5581 taatcagcgt gccttcaccg aaacggctga ctcggggccg tgtgctctcg cccgccagcg
     5641 catcacgtac gttattggga agcagcggtg ttgtcgccag ccattgggcg ctatcatggt
     5701 gtacataatt aaggtggagc cagcagggat gcgcttcatc aatcacatct gtattttcca
     5761 gcggtttaac gccgcctcta ccatccagca tccaggcaaa tactgcatcc gggacattaa
     5821 cgtccgatcc cttaatcgct tccactgtgc ctccatcatc aacgcattat tttgtagtct
     5881 agccttctgg ccctgttacg caacatctca tcaccccatt accctgaaat gattaataaa
     5941 attctgtcta aattgaatac aaaaagcaaa atgcttttcc gtatacaaac cgtgtgaagt
     6001 gttaaatagc gtctatcatt atcagaatta tctgatcata tgacgtggct tttttgcgat
     6061 cggatagcaa caaaaattga taaaaataac gggatctcaa tgattacgca caacttcaat
     6121 accctggact tactcaccag tcctgtctgg attgtttcgc cctttgagga acagttaatt
     6181 tatgccaata gcgcggcgcg actgttgatg caagacctca cgtttagtca gctacgaacc
     6241 ggaccctatt ccgtctcctc acaaaaagaa ctgccgaaat acctctccga tctgcaaaac
     6301 caacacgata ttatcgaaat cctcactgtt cagcgtaaag aagaggaaac agcattaagc
     6361 tgtcggcttg ttttgcgaga gctgacagaa acagaaccgg tgattatttt cgaaggtatc
     6421 gaagcgccgg caacgctggg tttaaaagcc agtcgctcgg caaattatca gcgcaaaaaa
     6481 caaggttttt atgcgcgctt ttttctgact aactctgcac caatgttgtt gattgacccg
     6541 tcacgagatg gacaaatcgt cgatgctaac ctcgccgcgc tcaatttcta tggttataac
     6601 catgaaacga tgtgccagaa acatacctag gaaataaata tgcttgggcg tcgcgtcatg
     6661 cctatcatgc atgaaatttc gcatttaccc ggcggtcata accctttgaa ttttgttcat
     6721 aaactggcgg atggatctac tcgtcatgtg cagacctatg ccggaccgat tgaaatttat
     6781 ggcgacaagc tcatgttatg tattgtgcat gatattactg agcaaaaacg gctggaggag
     6841 cagctggaac atgctgctca ccatgacgcg atgaccggat tactgaatcg gcgacagttt
     6901 tatcacatta cggaaccagg ccaaatgcag catctcgcca tcgctcagga ttacagcttg
     6961 ttgctcatcg acaccgatcg ttttaaacac attaacgatc tctatgggca ttctaaaggt
     7021 gatgaggtgt tatgcgccct cgcccgcccc ctcgaaagtt gcgctcgcaa aggcgatttg
     7081 gtgtttcgtt ggggaggcga agagtttgtc ttattgctac caagaacccc actggatacc
     7141 gcgctttcgc tggctgaaac tatccgcgta agcgtggcaa aagtgagtat ttcgggctta
     7201 ccacgcttta ccgtcagcat tggtgtggcg catcacgaag gaaatgaaag catcgatgaa
     7261 ctgtttaaac gcgttgatga tgctttgtat cgggcgaaaa atgatggacg caaccgcgtg
     7321 ctggcggcat aagccgcgga tgcgtctcga gatcaacgac tgcgcttagc gtggcgctcc
     7381 cagttttctt gcttcgcctg cgccgtttta cgtagtgcga cgtaacacgc cccgctgccg
     7441 ccatgatgcg gtagcgcggt gcaatatgcc tgaacatcat caaattcggt cagccagcgc
     7501 gccacatagc tgcggacaat attggcatgt gatttatctt cccgcccttt accatgaata
     7561 atcagcacat tacgcaaacc atccgccagg gcttgttgaa tgaaactgaa caccattttg
     7621 cggcactctt ccaccggctg gcgcaaaaga ttcaggctcg cctgttgcgg atatttacca
     7681 ctgcgcagct tatccagcac cccatgctac aacccttcct gccgaaactc cagcggctga
     7741 cttagcggga tgatgtcgag aaatccggta gtgaggaaat tatcaagctg cagcgtgtcg
     7801 atacgctgcg gcgcacgttg gttacgcgtt ggatgccagt ggacatctgt agcacgtttc
     7861 agcggctgga catcttccat ggcgtcaaga aacagcgatt tgtcgtcaag gttcatgtaa
     7921 cctcctatgc accttcacac gtcgtgattc agacgggtta attgttcata cttactgcat
     7981 cgttcccttc acagattgaa acaatactct acttcatacc tgtgagtatc tgcgcaggcg
     8041 gggcatttta tactagcctg ttagcagtat cgaacagttt agcccgagtt taattcagca
     8101 caggctccga tcaggatgat ttattcatag caggaaccaa caaaaatgcg ataaataaca
     8161 gaccactgat tagcagaaag gtaccgaaaa tgccatagtt tgcgcctccc tcagcatcaa
     8221 ttgactcaat aatttggttg aagccagtat ttccgatgtc tggtcacttc tactgcgatt
     8281 aaaaaataaa gcacttctgg aaatgaagtc attcaccgta tggggaacag aaaccatttt
     8341 ttcacccatt ctcgaaaaat gaaaattagt tcgattattg agaatagagc cacacagggc
     8401 aataccaatc gcgcccccaa gattacgtgt taaattaaat acaccagagg ctaacttcag
     8461 ccgttctttt ggaattcccc ccagcgttaa tgttacgatg ggggccatcg caaactgctg
     8521 cgaaatccct ctaattgcct gtggaaacag taattcctgc catccccatt cgtgggttat
     8581 aggggtaaaa agatacattg aaaacacaaa tccgcccagc cccgccatca acagccatcg
     8641 cagatttatt tttttactta accagaagta aaagggaaca gaaaataact ggaaaatacc
     8701 tgttgtgcaa acggcaaaac ctatctcttc ggcatttaat ccgcgaatct gccccaaaaa
     8761 cacaggtatt aaatatacag tagagaatat cccaacccct ccagaaaaag aaaaaaaaca
     8821 tcccagagtg aaatttttat ccttaaaagc atgaagatcc attatcggat tggatattgt
     8881 aagtgtacgc gcagcaaata aaataaaact caccaatgcc agaactgatg tcagcaaaat
     8941 agtattatca tccagccatc cccagcgcgc tccttcttca agggtatatt ccagacatcc
     9001 cagagtcatg gccaacagga taatactcgg ataatcagca actttcagta aagagagatc
     9061 aggtttatca aaattaacca gaaaaggaat actcaaaact aaatatatgc caggtaatat
     9121 attgatataa aatagccatc gccagtcgag gttatcagtg agccagcccc ccagagttgg
     9181 ccctagcgtc ggtgacagtg aggccagcgc actgactacc gcagcagcca accccaactc
     9241 tttaccctgg taataaataa acgccatcgt gaaaactaaa gggatcatgg atgcgccagc
     9301 agctccctgt aaagcacgaa acaaaatcat gatctgaata ttccatgcaa gtccacacgc
     9361 gatactcatt aaagtaaata ttcccgcgga tagagtaaaa agccaacgag tggaaaatac
     9421 ccgtgacagc cacccagaga gcggaataac aataatctca gcaattaaat agctggtctg
     9481 aagccaggcc atttcgtctt tacctgcgga cagaccacca cctatttcat ttagggatga
     9541 cgatacaatt tgaatatcaa gctgagcaat aaataatccc atacacatac tggcgaaagc
     9601 aatatgttta cgcatagaca tcatctctca tcaacactga cggtgacgga cagtcccggg
     9661 cgaagaagtt gctttaattc ctttgcgtcg tcaaatgcaa tccgtaccgg gacacgctga
     9721 acaattttgg tgaaattgcc agtggcattt tctatcggaa tcaagctgaa actggctcct
     9781 gtcgctgggg aaaggctttc aatgtgaccg tgaaacactt cccctttgag aatatcagca
     9841 cggatctcag ccttcattcc tggcttcatt cccgctattt ggttttcttt ataatttgca
     9901 tcaacccaca actcactgac tggtactagt gaaactaacg atgtgccgcc ttccacccaa
     9961 ctgcctgtat gggcgctgcg gtttgcgaca atgccatcaa tcggcgca
//
LOCUS       DAZPCS010000134         9944 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZPCS010000134 DAZPCS010000000
VERSION     DAZPCS010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9944
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.134"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..505
                     /locus_tag="ACHUQ7_003153"
     CDS             <1..505
                     /locus_tag="ACHUQ7_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR1136445.1"
                     /translation="KATRPNEKWVTDVTEFAVNGRKLYLSPVIDLFNNEVISYSLSER
                     PVMNMVENMLDQAFKKLNPHEHPVLHSDQGWQYRMRRYQNILKEHGIKQSMSRKGNCL
                     DNAVVECFFGTLKSECFYLDEFSNISELKDAVTEYIEYYNSRRISLKLKGLTPIEYRN
                     QTYMPRV"
     gene            602..754
                     /gene="hokE"
                     /locus_tag="ACHUQ7_003154"
     CDS             602..754
                     /gene="hokE"
                     /locus_tag="ACHUQ7_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFR1136446.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLAGDSLCEFTVKERNIEFRAVL
                     AYEPKK"
     gene            1105..1257
                     /gene="hokE"
                     /locus_tag="ACHUQ7_003155"
     CDS             1105..1257
                     /gene="hokE"
                     /locus_tag="ACHUQ7_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFR1136447.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(1379..1999)
                     /gene="entD"
                     /locus_tag="ACHUQ7_003156"
     CDS             complement(1379..1999)
                     /gene="entD"
                     /locus_tag="ACHUQ7_003156"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFR1136448.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(2174..4414)
                     /gene="fepA"
                     /locus_tag="ACHUQ7_003157"
     CDS             complement(2174..4414)
                     /gene="fepA"
                     /locus_tag="ACHUQ7_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HFR1136449.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKIGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKVETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SINTHF"
     gene            4657..5859
                     /gene="fes"
                     /locus_tag="ACHUQ7_003158"
     CDS             4657..5859
                     /gene="fes"
                     /locus_tag="ACHUQ7_003158"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFR1136450.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGLGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKVIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            5862..6080
                     /gene="ybdZ"
                     /locus_tag="ACHUQ7_003159"
     CDS             5862..6080
                     /gene="ybdZ"
                     /locus_tag="ACHUQ7_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HFR1136451.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            6077..9922
                     /gene="entF"
                     /locus_tag="ACHUQ7_003160"
     CDS             6077..9922
                     /gene="entF"
                     /locus_tag="ACHUQ7_003160"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFR1136452.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWVEQRRQLPPPASLSPAPLAGR
                     SASADILRLKMEFTDGEFRQLATQLSGVQRTDLALALTALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLATRLAAQLKKMRRHQRYDAEQ
                     IVRDSGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHG
                     DLSIEILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQIN
                     ATQVEIPETTLSALVAEQAAKTPDAPALADARYLFSYREMHEQVVALANLLRERGVKP
                     GDSVAVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQ
                     LPRFSDVPNLTNLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAI
                     VNRLLWIQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAM
                     QQFFAEYGVTTTHFVPSMLAAFVASLTPQTARQSCVTLKQVFCSGEALPADLCREWQQ
                     LTGAPLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPV
                     PPGVAGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEY
                     LGRSDDQLKIRGQRIELGEIDRVMQALPDVKQAVTHACVINQAAATGGDARQLVGYLV
                     SQSGLPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAP
                     GRAPKAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTP
                     GQVMVASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVL
                     SRYLDPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGIAARLRARGEQV
                     AFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGSTSTELFTTIEGN
                     YADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAELDIYRQDCAHVDI
                     ISPGAFVKIGPIIRATLNR"
BASE COUNT         2216 a         2573 c         2717 g         2438 t
ORIGIN      
        1 caaggctacg cggccaaacg agaagtgggt taccgatgtt actgaatttg cagtcaatgg
       61 gcgcaagctg tatttgtctc cagtaataga tctcttcaac aacgaagtta tttcttacag
      121 cctttcggaa agaccagtga tgaacatggt tgagaatatg ctcgatcagg cattcaaaaa
      181 gcttaatcct cacgagcatc ctgttctgca ctctgaccag ggatggcagt atcgtatgag
      241 aagatatcaa aatatcctta aagaacatgg tattaaacaa agcatgtcca gaaaaggcaa
      301 ttgtctggat aatgctgtgg tggagtgttt ctttggaacc ttaaagtcgg agtgttttta
      361 tcttgatgag ttcagtaata taagcgaact gaaggatgct gttacggaat atattgaata
      421 ctataacagc agaagaatta gcctgaaatt aaaaggtctg actccaattg aatatcggaa
      481 tcagacctat atgcctcgtg tataactgtc caactttttg gggtcagtac accctggggc
      541 tgccactcca aaccagacaa tttggatggt agtcccttct tcgcatggag gtaatataaa
      601 catgctgacg aaatatgccc ttgtggcagt catagtgctg tgtttgacgg tactgggatt
      661 tacgcttctg gccggagact cgctgtgtga gtttacggtg aaggaacgta atattgagtt
      721 cagggctgtt ctcgcttacg aaccgaagaa gtagccgttg tgcggggagt aatccccgca
      781 tatccggttg tcaggtcagg atggtaaggc acctgcttta cactttcgcc cgcggtcagt
      841 gatggctgcg ggcgatttcg ttttaagagc aatgatgagt tttgccgaca gaagtccgaa
      901 acaggactca ctgtataaat aaacagctat tttgctaagg aagggtaaga taacggcggg
      961 tgcctgaagc tttccggttt caggttaact ctgaggtttg gaaagatgaa gccccaagag
     1021 atatttctat caatcctggg gctgccactc cgaacctcaa caactcggat ggtagtccct
     1081 tcttcgcatg gaggcaatat aaacatgctg acgaaatatg cccttgtggc agtcatagtg
     1141 ctgtgtttga cggtgctggg atttacgctt ctggtcggag actcgctgtg tgagttcacc
     1201 gtaaaggaac gtaatattga gtttaaggct gttcttgctt acgaaccgaa gaagtagccg
     1261 ttgtgcgggg agtaatcccc gcatatccgg ttgtcaggtc agagtggtaa ggcacctgct
     1321 ttacactttc gcccgcagtc agtgatggct gcgggcgaat cgtaccagat gttgtcaatt
     1381 aatcgtgttg gcacagagtt atgactattt tttcttttat ctgccagtgc acagcaaaca
     1441 tctcattctc acgatgaatg atgacctgct gtttattcca gctaattatc tgatagtcca
     1501 gaaaacctgc atcagtttgg atctcacttg ccttaaatgc gctctctttg gcggaaaatg
     1561 caagtgtcag cgccagagaa aaggctaaac cgcagtctgc gagtcgctcg tgttctgctg
     1621 gtgtaataat gttgtctgtc aattcggtcg ctgtttgcgc agaaaaaatt tcttcgatat
     1681 caacgccaat cggttgacgg gaaaccacgg ccaatgccgt agccccgcag tggctaatac
     1741 tgccgtatac ctccgcaggc cacaccggtt ggcgtagctc gccgattgcg ggcacacatt
     1801 tatagccata ttcccgcaag gcataaacag cagcgatccg tccggctaaa tgctctgttt
     1861 tacgtttacg tccagcgtgt tgcagttgtg cgtagtgcgg cagccagagt aaatcctgct
     1921 cacaaaaatt cgccggatcg aactcaacaa aatgcagcgt atgtccggca aaggggaggg
     1981 aggtatgcgt agttttcata tcgaccatat tcgagactga tgacaaacgc aaaactgcct
     2041 gatgcgctac gcttatcagg cctacgtgat cctgcaatat attgaattgg caagatcttt
     2101 gtaggccgga taaggcgttc acgccgcatc cggcgtgaac gacgcgcact ttgtcaacaa
     2161 tctgacgtta gcatcagaag tgagtattaa tgctcatata ccacgtacgt cccggctcgt
     2221 tataggtata cgcgccggca ccggcgatat agttggctcc tgccagatcg cccgtggtct
     2281 gggcattacc tgcacgccac aaacgtttgt cgaacaggtt gtccacgccg ccggtcagac
     2341 tgacattctt cgtcacatcc caggtcgcgc tcagacccac aatgctgtaa ggactgattt
     2401 ctttggtttc cgggccaacc gctggctgac ctttatagtt gtacttcttc ggctgctgct
     2461 tgccgtacca ggtgaaggtc gtttgcatcg acaaatcttc ccgtgcctgc cagctcagcg
     2521 ttgagttcaa cgtgtactcc gggatgatcg acaaacggtc gcccgtggtt ttgttttcac
     2581 tcttcagcat ataagtgatg ttattggtcc acatcaccgt ttcgctaact ggtacgttta
     2641 acgatccttc cagaccttca accactgctt tcggcacgtt atcccactga tagagatcag
     2701 tgccgactgc gttttgccct acagccacat agcccgcttc aatcttattg cgataatcgt
     2761 tacggaacca ggtgacgccc gccagccagc cgtcgcgttt gaactccaga ccaatctctt
     2821 tgttgatgct ggtttctact ttcaggtcat cgttaccttg cagatagcag ccgcccgcgc
     2881 tggcatagca gccctgacct ttactgtaga gaatgtagtt cgggttagtc tggtataggc
     2941 tcggcgcttt ataggcgcgg gcaatgccta ttttcagcgt gaagtcatcg cctaaacctt
     3001 gcgatatgtt cagcgccggg ctccagttat tgccgacaat actgtgatga tcgaaacgca
     3061 gccccggcgt tacgatggtg ctgtcagtca gctccatgtt gttttcggca aacagcgaga
     3121 aaatttctgc ttttgaatac gggctacggt cggtggcact cacgccatca atagcgccac
     3181 cggtattcgt tccggtcagt gcttgggtgt tggaacttaa gtccttcatc cgttgctgat
     3241 tccactccgt acccagcgtc agcgtctggt taacgaggaa atcaatcggc aggttaactt
     3301 cgctgtgcag catcacgtca tcgagatcga tatcgacgaa atcctgtgtc gctttttcgt
     3361 taaatttccc ttcggtaccg cccgccagac cttccggaat acgcgagtta cgggtgtgtt
     3421 catactgcac ccagttgctg gtggtcacgc cgttatccca gccaccgttc caggtcagcg
     3481 cgtagttctg gcgatacagg cggttggttt catcgccata tttcgagcga gtgtaagcgt
     3541 cagagttggt gttctgggta tcgcccgcat acaggttacc ctggcggctg taacccgctt
     3601 ccagttccag cgattgcaat ggtgcgaagt cccagcgcac cacgccgtta atatctttat
     3661 tgatcacccc ttcgcgcccg gctggtaacg tcgtggcata cgttccggca cgcgcggact
     3721 gatggccctg gttgatatcc cacgcgtcag cctgagtttt gtcgaggttg ccatacaagc
     3781 ggaagctgaa ttcgtcgccc agcggaccgg tcaggctaaa gttagtgcgt ttggtggcac
     3841 cttcctcttt atgttctggc gcattgaaat atgcgtccca ggagccgtgc cactcgccgc
     3901 tgcctttttt ggtaatgatg ttaaccacgc cgcccgccgc gccgttgcca taacgcgcag
     3961 ctgccggacc acgcagaact tcaatacgtt caatcatttc aggtggcacc caggaggtat
     4021 caccacgggt atcgcgctcg ccacgccagc cctgacgcac cgagttacgg ctgcttaccg
     4081 gcttgccgtc aatcaaaatc agcgtgtttt ccggacccat accgcgaata tcaatctgtc
     4141 ggttattccc acgctgacca ctggtggagt taccggtcag gttaacgcct ggcatggtac
     4201 ggatgatctc cgacacatcg cgggcaaccg ggtttttgcg gatttcatct gcggtgatgg
     4261 tcgaaacgcc aggagcctgc aagttctgct cggcggcggt aacgacaatg gtatcgtcat
     4321 gtgaaacagg tgtatcggtc ggctcttgtg cctgcgctac cccataaatc cccagattga
     4381 ccaacaaggc cagggaatga atcttcttgt tcattgtttt attcctgcat ttttgccacg
     4441 aattgcaact gtcgggcatg gtcgtcatca acacgacgca tcccgttacc gcgaaaacct
     4501 ttgatcctga aagacacgca gtgcagttgg ttaattaatg tccgcgcttc ccacggcgcg
     4561 ccattacgct attgcaaatg caaatagtta ttaataacat tatcaatata tttctgcaat
     4621 caatgaaaaa ttgcacagta aacatggggt tatggtgtga cggcgttaaa agtaggaagt
     4681 gagagctggt ggcagtcgaa acatggcccg gaatggcagc gtctgaatga cgaaatgttt
     4741 gaggtcactt tctggtggcg tgatccccaa ggttctgaag aatactcgac gataaagcgc
     4801 gtatgggtct acatcactgg tgtgaccgat catcatcaga acagccagcc ccagtcgatg
     4861 cagcgaattg caggtactga tgtctggcag tggacgacac aactcaatgc caactggcgc
     4921 ggcagctact gctttattcc caccgaacgc gatgacattt tttctgcacc atcccccgat
     4981 cgcctcgaat tacgtgaagg ctggcgaaaa ctattacccc aggcaatagc cgatccgctg
     5041 aatccacaaa gctggaaagg cgggctaggg cacgctgttt ctgcactcga aatgccgcaa
     5101 gcgcctctgc aaccgggatg ggattgtccg caagcgccag aaataccagc caaagaaatt
     5161 atctggaaaa gtgaacggtt gaaaaattca cgtcgtgtat ggatttttac caccggcgat
     5221 gtaacagcag aagaacgccc gctggcagtt ttgctcgatg gcgaattttg ggcgcaaagt
     5281 atgcccgtct ggcctgtgct gacttcgctg acccatcgtc agcaacttcc tcccgccgtg
     5341 tatgtgttga tcgacgctat cgacaccacg caccgcgccc acgaactgcc gtgtaatgcg
     5401 gatttctggc tggcagtaca gcaagagtta ttacccctgg tgaaagttat tgcccctttt
     5461 agcgatcgcg ccgatcgcac cgtggtcgcc gggcagagtt ttggtgggct gtccgcgctg
     5521 tatgccggac tgcactggcc tgaacgcttt ggctgtgtat taagccagtc aggatcgtac
     5581 tggtggccgc atcggggcgg gcagcaagag ggcgtgttac ttgaaaagct aaaagctggt
     5641 gaagttagcg ccgaaggtct gcgcattgtg ctggaagcgg gtattcgcga gccgatgatc
     5701 atgcgggcca atcaggcgct gtatgcgcaa ttacacccta taaaagaatc cattttctgg
     5761 cgtcaggttg acggcggaca tgatgcgctt tgttggcgcg gtggcttgat gcaggggtta
     5821 atcgacctct ggcaaccact tttccatgac aggagttgaa tatggcattc agtaatccct
     5881 tcgatgatcc acagggagcg ttttacattt tgcgcaatgc gcaggggcaa ttcagtctgt
     5941 ggccgcaaca atgcgcctta ccggcaggct gggacattgt gtgtcagccg cagtcacaag
     6001 agtcctgcca gcagtggctt gaagcccact ggcgtactct gacaccggcg aatttcaccc
     6061 agttgcagga ggcacaatga gccagcattt acctttggtc gccgcacagc ccggcatctg
     6121 gatggcagaa aaactgtcgg atttaccctc cgcctggagc gtggcgcatt acgttgagtt
     6181 aaccggagag gttgatgcgc cattactggc ccgcgcggtg gttgccggat tagcgcaagc
     6241 agatactttg cggatgcgtt ttacggaaga taacggcgaa gtctggcaat gggtcgatga
     6301 tgcgctgata ttcgaactgc cagaaattat cgacctgcga accaatattg atccgcacgg
     6361 tactgcgcag gcattaatgc tggcggattt gcaacaagat ttgcgcgtcg atagcggtaa
     6421 accactggtc tttcatcaac tgatacaggt ggcagataac cgctggtact ggtatcagcg
     6481 ttatcaccat ttactggtcg atggcttcag ttttccggcc attacccgcc agatcgccaa
     6541 catttactgc gcattgctgc gtggcgaacc aacgcctgct tcgccgttta cgcctttcgc
     6601 tgatgtagtg gaagagtacc agcaataccg cgaaagcgaa gcctggcagc gtgatgcggc
     6661 attctgggtg gaacagcgtc gccaactgcc accgcccgcg tcactttctc cggcaccttt
     6721 agcggggcgc agcgcctcgg cagatatttt gcgtctgaaa atggaattta ccgacgggga
     6781 attccgccag ctggctacgc aactttcagg tgtgcagcgt accgatttag cccttgcgct
     6841 gacggctttg tggctggggc gattgtgcaa tcgcatggac tacgccgccg gatttatctt
     6901 tatgcgtcga ctgggctcgg cggcgctgac ggctaccgga cccgtgctca acgttttgcc
     6961 gctgggtatt catattgcgg cgcaagaaac gctgccggaa ctggcaaccc gactggcaac
     7021 ccgactggca gcacaactga aaaaaatgcg tcgtcatcaa cgttacgatg ccgaacaaat
     7081 tgtccgtgac agcgggcgag cggcaggtga tgaaccgctg tttggtccgg tactcaatat
     7141 taaggtattt gattaccaac tggatattcc tggtgttcag gcgcaaaccc ataccctggc
     7201 aaccggtccg gttaatgacc ttgaactggc cctgttcccg gatgaacacg gtgatttgag
     7261 tattgagatc ctcgccaata aacagcgtta cgatgagcca acgttaatcc agcatgctga
     7321 acgcctgaaa atgctgattg cccagttcgc cgcggatccg gcgctgttgt gcggcgatgt
     7381 cgatattatg ctgccaggtg agtatgcgca gctggcgcag atcaacgcca ctcaggttga
     7441 gattccagaa accacgctga gcgcgctggt ggcagaacaa gcggcaaaaa caccggatgc
     7501 tccggcgctg gcagatgcgc gttacctgtt cagctatcgg gaaatgcacg agcaggtggt
     7561 ggcgctggcg aatctgctgc gtgagcgcgg cgttaaacca ggggacagcg tggcggtggc
     7621 actaccgcgc tcggtctttt tgaccctggc actccatgca atagttgaag ctggcgcggc
     7681 ctggctaccg ctggataccg gctatccgga cgatcgcctg aaaatgatgc tggaagatgc
     7741 gcgtccgtcg ctgttaatta ccaccgacga tcaactgccg cgctttagcg atgttcccaa
     7801 tttaaccaac ctttgctata acgccccgct taccccccag ggcagtgcgc cgctgcaact
     7861 ttcacaaccg caccacacgg cttatatcat ctttacctct ggctccaccg gcaggccgaa
     7921 aggggtaatg gtcgggcaga cggctatcgt caaccgcctg ttgtggattc aaaatcatta
     7981 tccgctcaca ggcgaagatg tcgttgccca aaaaacgccg tgcagttttg atgtctcggt
     8041 gtgggagttt ttctggccgt ttattgccgg ggctaaactg gtgatggctg aaccggaagc
     8101 gcaccgcgac ccgctcgcta tgcagcaatt ctttgccgaa tatggcgtaa cgaccacgca
     8161 ctttgtgccg tcgatgctgg cagcatttgt tgcctcgctg actccgcaaa ccgctcgcca
     8221 gagttgcgtg acgttgaaac aggttttctg tagtggtgag gccttaccgg ctgatttatg
     8281 ccgcgaatgg caacagttaa ccggcgcgcc gttgcataac ctgtatggcc cgacggaagc
     8341 ggcggtagat gtcagctggt atccggcttt tggcgaggaa ctggcacagg tgcgcggcag
     8401 cagtgtgccg attggttatc cggtatggaa tacgggtctg cgtattcttg atgcgatgat
     8461 gcatccggtg ccgccgggtg tggcgggtga tctctatctc accggtattc aactggcgca
     8521 ggggtatctt ggacgacccg atctgaccgc cagccgcttt attgccgatc cttttgctcc
     8581 tggtgaacgg atgtatcgta ccggagacgt tgcccgctgg ctggataacg gcgcggtgga
     8641 gtacctcggg cgcagtgacg atcagctaaa aattcgcggg cagcgtatcg aactgggcga
     8701 aatcgatcgc gtgatgcagg cgctgccgga tgtcaaacaa gccgttaccc acgcctgtgt
     8761 gattaaccag gcggcagcca ctggtggtga tgcgcgtcag ttggtgggct atctggtatc
     8821 gcaatcgggc ctgccgttgg ataccagcgc attgcaggcg cagcttcgcg aaacattgcc
     8881 gccgcatatg gtgccagtcg ttctgctgca actgccgcag ttgccactta gcgccaacgg
     8941 caagctggat cgcaaagcct taccgttgcc tgaactgaag gcacaagcgc cagggcgtgc
     9001 gccgaaagcg ggcagtgaaa cgattatcgc cgcggcattc gcgtcgttgc tgggttgtga
     9061 cgtgcaggat gccgatgctg atttctttgc gctgggcggt cattcgctac tggcaatgaa
     9121 actggcagcg cagttaagtc gacagtttgc tcgtcaggtg acgccggggc aggtgatggt
     9181 cgcgtcaacc gtcgccaaac tggcaacgat tattgatggt gaagaggaca gctcccggcg
     9241 catgggattc gaaaccattc tgccgttgcg tgaaggtaat ggcccgacgc tgttttgttt
     9301 ccatccggcg tccggttttg cctggcagtt tagcgtgctc tcgcgttatc tcgatccaca
     9361 atggtcgatt attggcattc agtcgccgcg ccctcatgga cccatgcaga cagcgacaaa
     9421 cctggatgaa gtctgcgaag cgcatctggc aacgctactt gagcaacaac cgcacggcat
     9481 tgcggcgcgg ctgcgtgccc gtggcgaaca ggtggcattt cttggtttgc tggatacctg
     9541 gccgccagag acgcaaaact ggcaggaaaa agaggctaat ggtctggacc cggaagtgct
     9601 ggcggagatt aaccgcgaac gcgaggcctt cctggcggca cagcagggaa gtacttcaac
     9661 ggagttgttt accaccattg aaggcaacta cgctgatgct gtgcgcctgc tgacgactgc
     9721 tcatagcgta ccgtttgatg gtaaagcgac gctgtttgtt gctgaacgca cgcttcagga
     9781 aggtatgagc cccgaacgcg cctggtcgcc gtggattgcg gagctggata tctatcgtca
     9841 ggattgtgcg catgtggata ttatctctcc aggggcgttt gtaaaaattg ggccgattat
     9901 tcgcgcaacg ctaaacaggt aaattaatat tatctataaa ccca
//
LOCUS       DAZPCS010000135         9937 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZPCS010000135 DAZPCS010000000
VERSION     DAZPCS010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9937
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.135"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..1023)
                     /gene="bglB"
                     /locus_tag="ACHUQ7_003161"
     CDS             complement(<1..1023)
                     /gene="bglB"
                     /locus_tag="ACHUQ7_003161"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HFR1136453.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYYRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVG"
     gene            complement(1042..2919)
                     /gene="bglF"
                     /locus_tag="ACHUQ7_003162"
     CDS             complement(1042..2919)
                     /gene="bglF"
                     /locus_tag="ACHUQ7_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HFR1136454.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFGFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADAPGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGQLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHRGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTLMLH
                     FITSKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(3053..3874)
                     /gene="bglG"
                     /locus_tag="ACHUQ7_003163"
     CDS             complement(3053..3874)
                     /gene="bglG"
                     /locus_tag="ACHUQ7_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HFR1136455.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRLISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMHLVSAQ
                     MSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILEHASIN
                     DSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRKEH"
     gene            complement(4161..4886)
                     /gene="phoU"
                     /locus_tag="ACHUQ7_003164"
     CDS             complement(4161..4886)
                     /gene="phoU"
                     /locus_tag="ACHUQ7_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFR1136456.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(4901..5674)
                     /gene="pstB"
                     /locus_tag="ACHUQ7_003165"
     CDS             complement(4901..5674)
                     /gene="pstB"
                     /locus_tag="ACHUQ7_003165"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFR1136457.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(5766..6656)
                     /gene="pstA"
                     /locus_tag="ACHUQ7_003166"
     CDS             complement(5766..6656)
                     /gene="pstA"
                     /locus_tag="ACHUQ7_003166"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFR1136458.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(6656..7615)
                     /gene="pstC"
                     /locus_tag="ACHUQ7_003167"
     CDS             complement(6656..7615)
                     /gene="pstC"
                     /locus_tag="ACHUQ7_003167"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFR1136459.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(7702..8742)
                     /gene="pstS"
                     /locus_tag="ACHUQ7_003168"
     CDS             complement(7702..8742)
                     /gene="pstS"
                     /locus_tag="ACHUQ7_003168"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFR1136460.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(8990..>9937)
                     /gene="lpfD-O113"
                     /locus_tag="ACHUQ7_003169"
     CDS             complement(8990..>9937)
                     /gene="lpfD-O113"
                     /locus_tag="ACHUQ7_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="LpfA-O113 is the major subunit of a form of long
                     polar fimbria first described in LEE-negative, Shiga
                     toxin-producing enterohemorrhagic Escherichia coli (STEC)
                     of serotype O113:H21. This protein, designated LpfD-O113,
                     is the minor subunit of this chaperone-usher type fimbrial
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial minor subunit LpfD-O113"
                     /protein_id="HFR1136461.1"
                     /translation="SGYLITANENKVGTTFNTTVTNGSSYLGRCHCDTGNVGEFPYIY
                     YTSKINQALTYAGVHSNINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKY
                     NCNRIEPLSISSGAKAIVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMAD
                     VYIRGDITAPQSCEINNLKPVCFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLG
                     ILNTDDISTSFYATEPNTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMDKSTV
                     YGEKSGSVTFSAAPASLTGARPAPGQFTATATITVEIVR"
BASE COUNT         2563 a         2587 c         2295 g         2492 t
ORIGIN      
        1 tccaaccgga tcaatttgcc acccccactc tgaacttttc agatgcggat tggggatcat
       61 attcagtatg ttgccctgcg catttttatt aatgctttcg tcgtgggaaa cacaaccagt
      121 catgtaataa ctaaaagaga tgaaatctac agtatgtttt aaatcttctg cgtcactttc
      181 agtcatctca atggtgatat tgtggtcgcg gaagaaacgc tgcatatagc cgggatattg
      241 gccacgcgcc tgaacatcac caaagaacat ccagcgccgg ttctcttcca tggcctgcaa
      301 catatcctgt ggctggcagg tgagggggta aaccagccca ccgagaagca tattgccgat
      361 tttcgcttcg gggatcaggc tatgataggc tttaactgcc cgcgcactgg caaccagttg
      421 atgatggatc gcctgataaa cttccgcctc gccactctct tctgccagcc ccacgcccgt
      481 gaatggcgcg tgtaacgaca tattgatttc attaaacgtc agccataacg ctactttatg
      541 ttggtagcgg gtaaagaccg tgcgggcgta atgctcgaag tgatcgatga ccgcccgatt
      601 agcccaaccg ccgtagtttt tcaccagccc atatagcatt tcgtaatggg ataacgttac
      661 cagcggcttg atccccgcct gcgccatttc atcaaacagc cgatcgtaaa acgctaaccc
      721 cgcttcattc ggttcggctt cgtcgccctg agggaaaatt cgcgcccagg caatggaaat
      781 acgcagacag gtgaagccca tctcggcaaa taacgcgata tcttccgggt aacggtaata
      841 aaaatcgatg gcgacatctt tgatattctc tttccccagg atgcgcggtt ccatttttcc
      901 cattacgcca tgaggctgta aatctgaggt cgtgatccct ttgccatctt ccagccaggc
      961 accttccacc tgattggcag ctgttgcgcc accccaaaga aatgtttctg gaaatgcttt
     1021 cataattaac tccttttatc gttagcgaat gatggataac agcggttcac ctgcgcttat
     1081 ctgcgccgtg ccgtggggta atacgtctgt aaaatcatcg ctattactga ttaataccgg
     1141 cgtcgtcaga tcaaatccgg cctcgcgaat agcagggata tcaaaagaaa tcagccgatc
     1201 gcctgtattg accttgtcac ccacgttgac gtgagcggaa aagaatttgc cgtccagttt
     1261 tacggtgtcg ataccgacat gaatcaggat ctccacacca tcatctgact caatgccaat
     1321 ggcgtgtaat gtggcgaaca acgaagcaat tcgacccgca accggagaac gcacttcacc
     1381 aaccgagggc agaatggcga tacctttacc caacaggcca ctggcaaacg tggtatcagc
     1441 gacgtgaatg agcggcacaa tctctcccgt catcggtgaa cagataccgc cctgctcagg
     1501 tggtgtaata acctctggtg ttttctcttg cggggcaccc tgcgctggct gacgtttact
     1561 ggtgatgaaa tgaagcatca acgtaccaac aaatgcgcaa cctatggcaa tgacaccgcc
     1621 aataacgctg gcccagacgg tgaaatcaat ccccgttgac gggatggtct gcatgaaggt
     1681 gaaaatactt ggcaaaccaa aggagtaaac tttcgtttgc gcgtagccaa tgatggtggc
     1741 ccccaatgct ccactgatac aggcgataac aaaggggtac ttacgcggca ggttgacgcc
     1801 atataccgct ggttcggtga taccaaacag acccgtcaac gccgctgatc ccgccaccac
     1861 ttttttctgc gcatcgcgtt cgcagaggaa gacgccgagc gccgccccga cctgcgccat
     1921 aatggcgggc attaacagcg ggatcatggt gtcgtagccc agcacggtga agttattgat
     1981 acacagcggc accaggcccc tgtgcagtcc gaacatgacg aagatttgcc agaagccgcc
     2041 cattaccgcg cccgcaaatg caggaaccgc ctgataaagc cagagataac cggcggcaat
     2101 catttcgctt atccaggttg atagctgccc caccagcaga aaggtgacgg gtgtgataac
     2161 catcagacat agcaatggtg tgaagaaatt tttgattgcc gacggtaacc acgcattaag
     2221 tcggcgttcc agaatgctgc acaaccaggc agaaaaaata atgggaataa ccgatgacga
     2281 gtaattcaac aatgtgaccg gaatacccag gaaatccagc cccggcgcat ccgctttttg
     2341 cccgttctcg aaggcagtca gaattaatgg atgcactaac gctccaccaa tcaccatggc
     2401 agtaaatgga ttaccgccga agcgtttgcc cgcggtgtat cccaggatta tcgggaagaa
     2461 cccaaacaag gcatcactgg cgctgaataa aattaaataa gtaccacttt gttcggtcgt
     2521 ccactgaaaa gtgagcgcca gagccagcat acctttcagg atcccggttg ccgccatcag
     2581 accgatcaga ggcgtaaaaa tacctgaaat aacataaaca aagcggttta gcagattacc
     2641 tttctcatca ttttccggtg cctgttgcgc tttttcgcca aggcctgcca cgctgttaac
     2701 cgccaggaag acatcggcca catggttacc tatgaccacc tgaaactgac caccgctttc
     2761 caccaccata ataataccgg gggtcttttt cagtacctca gcttgcgctt tgctttcatc
     2821 ctttaactta aaacgtaatc gcgttgcgca atgcatcaga ctcacaatgt tatctgcgcc
     2881 cccgactcct gcgactattt ttctggctaa ctccgtcata acttgccctc taccgctttg
     2941 cggcaaaact ccaaaaaaaa acctgaaaaa aacggcctga cgtgaatcaa gcaatttttt
     3001 tcaggttttg cccgcttagt gcggtaacaa tcctttactc agtaataata tttcagtgtt
     3061 ctttgcgcac gcgctctata tttatggcta aaaacataat ctctgcgggt gaaattttac
     3121 gttgatactg caaaccaata aaaatggcga tccgttccgc acattgccat gcttgcgggt
     3181 aattttgttt tactgcttgt tgtaatgatt catcactatc gttaattgaa gcatgttcaa
     3241 gaatacgcca ggataaaaac ttcagatgtg taaccagtcg ctgataactc aagctttcct
     3301 cctggtaatt aaggctgaac tgaaatttta ttaattgcag catttcgcgc attaactgcg
     3361 tgacacctgc aacatcctcc atatttccgc tcatttgggc actgaccaga tgcatggcaa
     3421 taaagcccac ttcatctttc ggtaactgca cgcccaaccg tttatcaata atggttaacg
     3481 cttcttcccc tagctggaac tctttcgggt aaagccgctg gatatcccac agcaacacgt
     3541 tttgctgaaa gcgtttaatc gcaaactggc aatggtcagt tagcgagata taaatactgt
     3601 cctgtaattt tcccaagcgc tcctgcgcta aagagataag acgatcacag gttgccatca
     3661 cctcaagagg aatatgactt aagagttcgc ttaatcgccc gttcagttca tgactgctca
     3721 aggcatactc tttttctatt ccacttgagt taattctttc gccagcgcgt ttttgaaagc
     3781 caattccgcg ccccatgacg actttttccc gctgttgatc atcaataacc accacaacat
     3841 tattgttgag aattttggtg atttgcatgt tcatagcaag gacctttttt ataaacaaaa
     3901 aaaaaccgac ttcaccagta ttctctggtt atgtcaggtt ttgcctgcga atgcagtaac
     3961 aatccagtca tttattaatg gtttttataa cgaacatcca ggttcggaaa ttaatttaat
     4021 tgcgtgcaga gaaaatagca atgcgctatt gataaaaata tgaccatgct cgcagttatt
     4081 aactttgtgt aattttagga atttataaag ttatatataa caaatcccaa taattaagtt
     4141 attgggattt gtctggtgaa ttatttgtcg ctatcttttc ccgccagcag tttatccagc
     4201 tcatcaccgc ctacatgacg gaaatcctga cccttcacgt agtagaagat aaactcacaa
     4261 atattctggc agcggtcgcc gatacgttcg attgaacgcg cacagaacag cgcggtaagt
     4321 acgctcggaa tagtgcgcga atcttccatc atgtaggtca tcagttgacg aacaatacct
     4381 tcgtattcct gatcgacttt tttatcttca cgataaatac gtaccgcttc gtcaatgtcc
     4441 atccgcgcga acgcgtccag cacgtcgtgc agcatctgga tggtatgacg gcccagcgac
     4501 tccagactta ccagcaacgg ctgatgctgc tgggagaatt tctccagcgc agtacggcag
     4561 attttgtccg ccacgtcgcc aatacgctcc agctcggcaa tggttttact gatcaccata
     4621 accagtcgca ggtcgctcgc cgtcggctga cgtttggcga taatgcgcac gcaggcttca
     4681 tcgatcgcca cttccatcat gttgacgttc ttgtcgcctt cgatcacgcg cttcgccaga
     4741 tcgctgtcct ggttatgcat cgcggtgatt gcatcagaaa gctgctgctc caccatgccg
     4801 cccatggtca tcacctgcgt gcggatactt tccagttcgg cgttgaactg gccggaaata
     4861 tgtttattaa gattgagact gtccataacg cactcctgaa tcaaccgtaa cgaccggtga
     4921 tgtagtcttc tgtttgtttc ttcgctggct tggtgaacag atcgtccgtg ttgctgaact
     4981 caatcaattc gcccaggtac ataaatgccg tgtggtcgga acaacgcgca gcctgctgca
     5041 tgttgtgggt gacgatcacc acggtgtaat cctgcttcag ttcggtgatc agctcttcaa
     5101 tacgcccggt agagatgggg tcgagcgccg aacacggttc gtcgagcagc agcacttccg
     5161 ggcgaatggc gataccacgc gcaatacaca gacgctgttg ctgaccacca gagagagagt
     5221 aaccgctctg gtgcaattta tctttggttt cgttccacaa tgcggctttg gtcaatgccc
     5281 actgcacgcg ctcgtccatg tcggcacggg agagcttctc aaacagacga acgccaaagg
     5341 cgatgttgtc gtagatggac atcgggaacg gcgtcggttt ctggaacacc atgcccactt
     5401 tcgcacgcag cagggcgata tcctgagagt tggtcaggat gttgtcgcca tcaagcagaa
     5461 tttcaccttc cgcacgctgc tccgggtaca gttcaaacat tttgttgaag gtacgcagca
     5521 gcgtcgattt accgcagccg gacggcccga taaacgccgt aacctggttt ttggcgatat
     5581 ccaggttgat gtttttcagg gcatggaatt tgccgtagta gaagttcaaa ttacgaacct
     5641 gaattttact cggggcagtt tcaaccatac tcattgcaat cttttcctca tcgtacctga
     5701 tgcttttcgc taatcaggct taccgtaggg ccgggtaagg cgttcacgcc gcatccggaa
     5761 aagtgttaac cgtgtttatt cttcgcaaaa acaacgcgcg ccagaatgtt cagcagcagg
     5821 acgcacaggg taatgatcaa taccccggcc caggccagtt gttgccattc cgcaaacggg
     5881 ctcatcgcaa acttaaagat cgtcaccggc aggttggcga tcggctgcat catgtccgtg
     5941 ctccagaact ggttggagag cgcggtaaac agcagcggcg cggtttcacc ggcaatacgg
     6001 gcaatcgcca gcaggatacc ggtcataatc ccggacaccg acgctttcag cgtaatcgcg
     6061 gagatcatct tccacttcgg tgtacccagc gcataagccg cttcacgcag gctgtccggc
     6121 accagtttca gcatgttctc agtggtgcgg ataacgatcg gcacctgcaa cagcgccagc
     6181 gcaattacgc ccgcccagcc ggagaagtgc tccatctgtg ccaccacgat ggtgtagaca
     6241 aacagaccaa ccacaatcga cggcgcggag agcagaatgt cgttaatgaa gcgaatcact
     6301 tctgccagcc aggatttacg accatattcc gccagataaa tccccgccat aatgcccagc
     6361 ggcgtaccga ataccgtggc ccacaaaatt aacagcccgc tacccgccag ggcgttcgcc
     6421 agaccaccac cttccgtatt gggcggcggc gtcatttcag tgaacagcgc cagcgacata
     6481 ccgtcgatac cgcgagtgat ggtggacatt aaaatccaga tcagccagaa caggccgaag
     6541 gccatcgtcg ccatcgagag cgtcagcgca atacggtttt tgaggcggcg acgcgcctgc
     6601 attttgcggc gagattcagc cagcgccgca gtggtttgca tttcaaccat agccattagc
     6661 gtgccccctc attcttagcc aggcgcataa tcataaactt cgatgcggcg aggacgatga
     6721 aggtaatcac aaacaggatc aggcccagtt ccatcagtgc ggcaacgtgc agaccggatt
     6781 ccgcttccgc aaattcgttc gccagcgcag aggtgatact attgcccggc atgtacagcg
     6841 aggcgctgtc gagctggtag gtgttaccga tgataaaggt caccgccatg gtttcaccga
     6901 gcgcgcggcc cagccccagc atgatgccgc cgataacacc atttttggtg aacggaagaa
     6961 cgatacgcca gataacttcc caggtggtgc agccaatacc gtaggccgac tctttcatca
     7021 tcaccggggt ttgttcgaac acatcacgca ttaccgccgc aatgtacgga ataatcatga
     7081 tggcgaggat cacgcctgcc gcgaggatac cgataccaaa tgcggggcca gagaacagcg
     7141 cgccaacaat cgggatattc gacatgatat tgccgaccgg ctcctgaaag taaacggcga
     7201 acagcggcgc aaagataaac aggccccaca tgccgtaaac gatacttgga atggctgcca
     7261 gcagctcaat ggcgataccc agcgggcgtt tcagccagcc aggcgcaagc tcagtcagga
     7321 acagggcgat accgaaactc accgggacgg cgatcagcag cgcgataaac gaagtcacca
     7381 acgtaccgta gatcggcacc agcgccccgt agatatcgtt cggtgcatcc cactctttgg
     7441 tccataggaa agccagacca aatttctgaa tgctcggcca ggaggagatg atcagagaga
     7501 caataatgcc acccaacatc aatagcacaa tcagcgccgc cagttttacc agcacgctga
     7561 aaattatgtc gcccttttta cccggtgggt taaaagcggg cttggttgca gccataagtt
     7621 actcttcagt taaacgcgtt tacaaagtca ttttgtaatg ccggatgcgg cgtaaaacac
     7681 cgtacccggc ctggagtttt attagtacag cggcttaccg ctactgtctt taatattggt
     7741 cttccacgca gcgcgaacct gttcaactac actatccggc aggctggcgt aatccaggtc
     7801 gttcgcctgt ttagccccgg ttttgtacgc ccagtcgaag aatttcagca cctctgtgcc
     7861 ttgttctggt ttcttctgat ctttgtggat cagaatgaac gtggtagagg taataggcca
     7921 tgcatcttcg cctttctggt tggtcagatc ctgagcgaag gttttgctcc agtctgcacc
     7981 ttttgctgca ttagcgaagt tttcttcggt cggactaacc ggtttaccat cagcggagat
     8041 cagtttggtg tacgccaggt tgttctgctt cgcgtaagca tattcaacat aaccaattgc
     8101 acccggcaga cgctgaacga acgcggcgat accgtcgtta cctttaccgc ccagaccgat
     8161 cggccatttt acggtagagc cagtaccaac gttgtttttc cactcttcgt tcactttcgc
     8221 caggtagctg gtgaagacga aggaagtccc ggagccatct gcgcggcgta ctacagcaat
     8281 gttttgtgaa ggcagtttca gacccggatt cagtttggcg atggcttcat catcccactt
     8341 cttgattttg cccaggtaga tgtcgccgag ggttttacca tccagcacca gttcgccaga
     8401 cttcagccct ggaatgttaa ccgccagcac cacgccgcca atcacggtcg ggaactggaa
     8461 cagaccttcc tgagccagtt tttcgtcaga cagcggcgcg tcagaggcac caaaatcaac
     8521 ggtattagcg ataatctgtt ttacgccacc ggaagaaccg ataccctggt agttaacttt
     8581 attaccggtt tctttctggt aagtgtcagc ccatttggca tacaccggcg cagggaaggt
     8641 tgcacctgca cctgtcaggc ttgcttctgc aaacacagag aaagcactca tcgataaggt
     8701 cgcggcgaca acagttgcga cggtggtacg cataactttc ataatgtctc ctgggaggat
     8761 tcataaagca ttgtttgttg gctacgagaa gcaaaatagg acaaacaggt gacagttata
     8821 tataaggaat atgacatttt tatgacagaa aggaattacc aaataactta ctgattttaa
     8881 atgtgaagat gaaaattcca catgccatct ttttgtcatg atgcatttaa aagtgataaa
     8941 gaaatgggtg agatgacaac gatgtgatga acaatcgttg tcagacctat tagcgcacaa
     9001 tctcaacagt tatcgtcgct gttgcagtaa attgccccgg ggctgggcga gctccagtaa
     9061 ggctcgcagg agcagctgaa aaagttacac tacctgattt ttcaccatac actgttgatt
     9121 tatccatgtc tgttggcaac tcaccaccgt tcactttgat ttccttatta tttttgtcgt
     9181 aaatttttat ccccacgtta gagtttgaag ttacgaccat tgaattgtcc gtattgggtt
     9241 cggtagcata aaaagacgta ctgatatcat cggtattcag tatccccagg ttttcacact
     9301 caatagtgac tgttttagta attttatgtg ttgttaccgc acttccaaca acagatgaaa
     9361 aatctgcagc tggtatttct ttaaaatcaa aacaaactgg ctttaaatta tttatttcgc
     9421 aactttgcgg agctgtaata tcgccccgaa tataaacatc agccatcggc tgtgagtaat
     9481 ccaccggggt gtcacggcta attgttccat acaatgtcac tatctttgtt tcaggaataa
     9541 tcaattttcc tgcaaatgtc ttcttgatat aaaaatatac tatcgcttta gccccactgg
     9601 atatacttaa aggttcaatg cggttgcaat tgtactttgt attaccagac gggttgttag
     9661 catgatattc aaaaggtgca ttcacatacc caaccccaag aatatctata gcaatcccaa
     9721 catcaaggtt cggattaaga tcataataat taatattaga gtgaacccca gcataagtta
     9781 atgcctggtt tatttttgac gtataataga tataggggaa ttctcctacg ttaccagtat
     9841 cacaatgaca gcgtccaaga tatgaggatc cattagttac agtggtgtta aatgtcgttc
     9901 caaccttatt ttcgttcgcg gtaataagat atcccga
//
LOCUS       DAZPCS010000136         9841 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZPCS010000136 DAZPCS010000000
VERSION     DAZPCS010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9841
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.136"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(119..1459)
                     /gene="gudD"
                     /locus_tag="ACHUQ7_003170"
     CDS             complement(119..1459)
                     /gene="gudD"
                     /locus_tag="ACHUQ7_003170"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFR1136462.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(1480..2820)
                     /locus_tag="ACHUQ7_003171"
     CDS             complement(1480..2820)
                     /locus_tag="ACHUQ7_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFR1136463.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(2822..4174)
                     /gene="gudP"
                     /locus_tag="ACHUQ7_003172"
     CDS             complement(2822..4174)
                     /gene="gudP"
                     /locus_tag="ACHUQ7_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFR1136464.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPVQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQSTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(4608..5057)
                     /locus_tag="ACHUQ7_003173"
     CDS             complement(4608..5057)
                     /locus_tag="ACHUQ7_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFR1136465.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(5075..5857)
                     /gene="truC"
                     /locus_tag="ACHUQ7_003174"
     CDS             complement(5075..5857)
                     /gene="truC"
                     /locus_tag="ACHUQ7_003174"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFR1136466.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(5857..6186)
                     /locus_tag="ACHUQ7_003175"
     CDS             complement(5857..6186)
                     /locus_tag="ACHUQ7_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFR1136467.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(6808..7353)
                     /gene="syd"
                     /locus_tag="ACHUQ7_003176"
     CDS             complement(6808..7353)
                     /gene="syd"
                     /locus_tag="ACHUQ7_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFR1136468.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTNLASNLAEFLNQLKPLL"
     gene            7421..8269
                     /gene="queF"
                     /locus_tag="ACHUQ7_003177"
     CDS             7421..8269
                     /gene="queF"
                     /locus_tag="ACHUQ7_003177"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFR1136469.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            8381..9745
                     /gene="ppnN"
                     /locus_tag="ACHUQ7_003178"
     CDS             8381..9745
                     /gene="ppnN"
                     /locus_tag="ACHUQ7_003178"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFR1136470.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHHTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         2454 a         2681 c         2323 g         2383 t
ORIGIN      
        1 caattaaatt caatgaatta aaaaatgatg tagacctgat acatagcaca acaggctatt
       61 ttgcgtttag catcagtctc aaaccggctc cagatagagc cggttttggt tttctgtctt
      121 aacgcaccat gcacgggcgc ttgttatcga acgtccagcc aggaatcaga tactgcattc
      181 ccatcgcatc gtcacgcgcg ccaagcccgt gtttctgata cagctcatgg gctttcatca
      241 cttgatccat atcgatttct acacccagcc ccggtttttc tggcacctgt accagcccgc
      301 ctttgatctc aaacggttct ttggtcaggc gctgattacc ttcctgccag atccagtgcg
      361 tatcaatagc agtaatttta cccggtgcag cggcggcaac atgggtaaac atcgccaggg
      421 aaatatcgaa gtggttgttt gagtgtgaac cccaggtcag gccaaattca tggcacattt
      481 gcgccacacg taccgaacct tgcatagtcc agaaatgcgg atccgccagc gggatatcaa
      541 cggattgtaa cgagagcgta tggcccatct gccgccagtc ggtggcgatc atattggttg
      601 cagtcggcag gccagtcgcg cgacggaact ctgccatcac ttcacgcccg gagaaacctt
      661 gctccgcacc acacggatct tctgcataag ccagcgaacc tttcaggtat ttaccaattt
      721 taattgcttc gttcagcgac caggcaccgt taggatcgag cgtaatacgc gcctgcggga
      781 agcgttgcgc cagtgccaca atagactcgg cttcttcttc cccggccagt acaccgcctt
      841 tcagtttgaa atcgttgaag ccatattttt catacgccgc ttccgccagg cgcaccaccg
      901 catccggcgt catcgcttct tcatgacgca ggcgatacca gtcgcatgag tcatccggct
      961 ggctttgata cggcagaggt gtggctttgc gattaccgac gaagaacaga taaccgagca
     1021 tttcgacttc gctacgctgt tgaccatcgc ccagcagcga tgccacgttt acccccagat
     1081 gctgccccag cagatccagc attgccgctt ctatcccggt aactacatga atagtggtac
     1141 gcaaatcaaa tgtctgcaaa ccgcgcccac tagcatcacg atcggcaaaa gtattacgca
     1201 ccagcgtcag aacgtttttg tattcaccca gcgttttacc taccaccagc ggaatcgcat
     1261 cttccagcgt tttacggatt ttctcgccgc cgggaatttc ccctacgcca gtgtgaccgg
     1321 aattatcttt gataatcaca atattacgcg tgaagaacgg tgcgtgtgca ccactcagat
     1381 tcatcagcat actgtcatga cccgccaccg ggatgacctg catttcagta acaacaggcg
     1441 tcgtaaattg agaactcata tttatgtcct ttttcagaat caatgacggc cgaaaacggg
     1501 acgtttacgg tcaaaggtcc agccggggat caggtactgc atcggacctg cgtcgttacg
     1561 cgcaccgcaa ggcagacgtt tataggcctc atgtgccttt tgtacctgtt cccagtccag
     1621 ttccacgccc agaccgggcg catcaggaac ggcaattttt ccgtttttaa tctccagcgg
     1681 atttttggtc aggcgacaat cgccctcctg ccaaatccag tgggtatcga tagcggtagg
     1741 attacccggt gccgccgcgc ccacatgggt aaatatcgcc agagagatat cgaaatggtt
     1801 gttggaatgg cagccccagg tcagccccca gtcgtcgcaa agctgcgcca cgcggactgc
     1861 accggaaagc gtccagaagt gcggatcggc aagtggaata tctaccgcat tgagcatcac
     1921 cgcatgaccc atttcgcgcc agttggtggc gatcatgtta gtcgcgacgg gcaagccggt
     1981 cgcccgtcga aactccgcca tcacttcacg tccggagaag ccctgttctg cgccgcatgg
     2041 atcttcggca taggtaagaa catcattcag ccctttgcat aaagaaatgg cttcatcaag
     2101 cagccatgca ccgttgggat caacggtaat ccgcgcatcc gggaagcttt tcttcaatgc
     2161 acgaacagtg tcgatttctt gctcgccagg taacaccccg cccttaagtt tgaaatcttt
     2221 aaagccatag cgatcctgtg aggcttccgc cagacgcaca acggcttcgc tgttcatcgc
     2281 tttctgatgg cgcaactgat accactcatg gttgcccggc gtattttcca gataaggaag
     2341 atcggttttg gtccgatcac ctatataaaa cagataaccg aggacggtaa tagtctcgcg
     2401 ttgcttgcct ggccccaaca gttcgcagac cggaacattc agcgccttac ctagcaggtc
     2461 aagcaaggcg gcttccagcg ccgccacggc attaacgcgc aattcaaaag tccaggcacc
     2521 tttgccaaag gtatcaaaat cggctgcctg attaccttta tgtacctgct gaaccacttt
     2581 attcaggcgc gcaacttcct ggcccagaac catcggaata gcatcgacca gtgtttgata
     2641 aatcacctct ccgcccggcg cttcaccaat gccggtatgc ccggcgttat cggtgagtac
     2701 cacgatattg cgggtgaagt atgcgttatg cgcaccacca atattgagca acatgctgtc
     2761 atgtccggcg accggaatga ctttcatatc agtaataaca ggactggatt gtgtcgccat
     2821 cttattgccc cgcgacaggt ttcagctcga tacgcttgat atcgcccacc agcaccaggt
     2881 agctcagtac cgcgattagg gcatgaacac caacataaat cagcgcccca ttaaacgagc
     2941 cagtcgtgcc aacgatataa ccaattgcga ttggcgtgac gatgccagaa atgttaccga
     3001 acatgttgaa caggccaccg gaaagaccgc tgatctcttt tggcgcggta tctgccatta
     3061 ctgcccaacc cagcgccccg atgcccttac cgaagaaggc cagcgccata aagccgatga
     3121 tcatccactc aacgttgacg tagttgcaga acaccatcac catcgacagc aacatgccca
     3181 ttacgatcgg tgttttacgc gcaatgttca gcgatcccgt gcggcgcatc agccagtcgg
     3241 aaataattcc acccagcaca ccgccgataa aaccgcaaac cgccggaacg gaagccacaa
     3301 agcccgcttt cagaatcgac atcccacgcg cctgcaccag ataaaccgga aaccaggtaa
     3361 tgaaaaagta agtcagggcg ttgatacagt actgaccgat ataaacgccg atcatcatcc
     3421 gtgacccgag cagctgtttg atctgccccc acttcacgct gaacggcact ttaactttgg
     3481 tgctttgctg atccatattg atcagcgcgc cacccgcggc gatatactcc agctctttct
     3541 gatttacccc cggatgttga tttggctcgt ggatgacttt caaccagata aagctgatga
     3601 caatgcccag accgcccata aagaagaaga cgtgtgacca gcccacttca tgcgtcagcc
     3661 agcccataat cggcgcgaag atcaccgttg cgaagtattg agcggagtta aaaatcgaca
     3721 ccgccgttcc cctttcctgc accggaaacc aggccgcaac aatgcgactg ttgccgggga
     3781 aagatggcgc ttcagcaagc ccgaccagga agcgcagagt aaacagggca acgataatgc
     3841 cgaatccact aaagatatcg acgaagcctt gcagcaaggt aaacatcgac cagataaaga
     3901 tcgaccagaa gtagacgcgt tttgaaccaa aacgatccag caaccaacca ccagggatct
     3961 gcccgataac ataagcccat gagaaggcag agaacacata gcccattccc acgggatcaa
     4021 ggccgatatc tttggccatt tccgaaccgg caatagaaag cgtagcgcgg tcgccgtagt
     4081 tgaaggatgt gacgataaac aacatcacca ctatccagta acgagcattc gtgcgttttt
     4141 ccacactgct cgcagcctga cttaaagaac tcattgttgc actcctgaaa attcgcgtta
     4201 gccacgctca ctctggactg cgacatcgcc aggaaatcag aggtgacgta gggtgttttt
     4261 tgccgttttt ataggtcgtt cgccgaatac ggcgcgtgtt tatatctggc aatagcagta
     4321 taaaaagcgc gccatagcgg ctcaccgtgc aacaacacaa cattaatgcg ttcaatgagg
     4381 cccgattttg gcattagccc tggacggtgg aatccacttc acggaaatga aaacaagaac
     4441 aagaaaggaa gggtaaaaac gaagaaataa agaagagtat gaaatggatc gcttgactct
     4501 aggcaaacgc cagtaaaaat ccgcgctatg aagcagtttt tactggcatt tgcctgaaaa
     4561 gattcgattc agcaccgcta aaacgacatt taccgctcgc tgacatatca ggacaacagc
     4621 gtgccccact gttcgaccca cggatttgat tccgtttccg gttccgggtt ctcactggcg
     4681 tcaatcaaca gcatttcgcc aacccgctga gcgctctgtt cctgcaacaa ggcatcgaac
     4741 tgtttgccgc cattgcaaaa atttacataa ctgctgtcgc cgagcgcaat cacgccataa
     4801 cgcagattcg gctggaagcc cagactatct ttgattccct gaaagagcgg cacaatgcta
     4861 tcaggaaggt cgccctgccc ggtcgtggac gtaaccacca gaacatactt atcctgatag
     4921 ggcagccagt cgcttaattc aggatcttca aataccgttg ctttgtggcc ctgtgcggtc
     4981 agaattgctt ctgcttcttc cgccactaac agcgaatttc cgtacatggt gccgacaaaa
     5041 ataccaattt ccgccatacc gttttctccc tggattagga acttatctct ccatcctgac
     5101 ccgatggcgc actgaactca accctttcat tttcaggaag cagaccgcgc cagccaaatt
     5161 gtgataacgc ctgcatccag gtgtcgtcca ggcccgcgtg aatagtcagc ggctcgccag
     5221 taaaaggatg cgtcaatgac aactgactgg catgtagcat taaccgctgg aggccaaaat
     5281 gctcagcacc gctgcgattc tggcgtaaat cgccatgttt gctatcgcca ataatcggat
     5341 gacgcaaatg ggcaagatgt cgtcgaagct gatgtttgcg tccggttttc ggctccagtt
     5401 ccaccaggcc gtagcgcgtg gtcgggtaac gtccggtcgc taccggcatt tctacggtcg
     5461 ccagaccgcg ataatgcgtc actgctggct gcgggccttt atcttcgcgg gcaaatttat
     5521 cagcgatttt gtccagttct tccaccagtg gataatccaa caccgcttct tccatcaacc
     5581 agccgcgcac aatcgcatgg taacgtttct ggatttggtg ctgttcaaac tgttgtgcca
     5641 gcagccgtcc ggcctcgctg gataatccca tcaacaacac accagaagtg ggtcggtcca
     5701 gacgatgagc agtaaaaaca tgctgtccta tctggtcacg cacggtttgc atgaccacta
     5761 ctttctcgtc gcgatccagc cagctacggt gaaccagcca gccggagggt ttatttaccg
     5821 caaccagcca ttcatcctga tagagtattt ccagcattag ctcgcatcat ccgcaaaaag
     5881 agcatccagt ttttccaact cagccagaat aagcgcgcgt tgcggatggt ccgtcgccag
     5941 cgccatttca taatagggtg caacagcaaa agcgcccggt aacggctggt tgttatttaa
     6001 caaatcgtgc attcgcggga tcagccccca ctgcaaccac tccagtggtt ccatggtgtc
     6061 cataaagaac ggttgggtac tattaaattg atgcggctgg ggttcatcgt ttcgccagtg
     6121 ctgatgttca cgcagtaacg cttcaagcgc ctgcaactgg agacgaacgc ggtcatgagt
     6181 ggtcataaag caacctcaat aagaaaaact gccgcgaagg atagcaggaa taaaaaaagg
     6241 gagcactgta ttcacagtgc tcccggttcg tttcgcagca ttccagctac ttttgttgct
     6301 ccctgctcat ccttgacaac ttttcctctg gccttgcggc caatcgttca tcctgaacta
     6361 ttgcttcctg ctcacaccac cccgatgtga tacttcatcc tgaagtgtcc ctggccttcc
     6421 tgacccaccg aatcatcctg accggttctc attctccatc ctggaggtgt cctttaacgc
     6481 gtcctgcgtc atcctcttcg cttcatccag aagcctttcc ctgaaacacc atcctggtgt
     6541 gtcctgcaga agtgtcatca tcctgatgtt cacttcgttg tctgactccc tgtcgacgaa
     6601 gatagaatcg tctttttcgc gaagtcttac aaggcgctta cagacaatgc cttaaggaaa
     6661 atttcatatg aaagttaaga attaacaatc aacattttga ttttaaatga aaataacgat
     6721 tcttatcttt ttaagtattc gcttatttcc tggcgatctc ctacagagtc tgtaagagat
     6781 ctctcacaac cagatggggt atttggatta cagaagaggc ttaagttgat taaggaattc
     6841 cgcaagattt gaggctaaat tcgtccgttt acgtgttccc agcgtctctt tgcacacttc
     6901 accggagaga ttacacaccg agatgacctc taactcttct tccagcgtcg cgataaatag
     6961 cgtgggcggc agttttagcc ttttctgagt caccagatga ccaatcagat tttcctgcac
     7021 ccgacggaag tcgtcttcac tccaggtttg cagcaatgtc agtttgatat cgccaaactg
     7081 cgcgtgcata tccccggcaa actgagtcgt ataaaaggta tgaattgtgg gttgtatcac
     7141 aatatcaaaa gcgcgttcaa ccgcgtttac attttgttcc ccggtaaacg gctggggttg
     7201 ccagtatacg gcatcttcgg tagttgaaat aatgcacggt gaaggaacac cgtacaattc
     7261 ctcacttaac ggccagcttt tatgctcttc atgccacgca tcgcagtagc gtgcagtaaa
     7321 atctttcagg gcttgtgcgg tcaaatcgtc cactaatttc tctcttcact taaaccagat
     7381 acacttgtcg tttagtttat ctggtttatg acggtgaaac atgtcttctt atgcaaacca
     7441 tcaggcactt gcgggcctga ctcttggaaa atcaaccgat taccgggata cctatgacgc
     7501 cagcctactg caaggcgttc cacgcagcct gaatcgcgac ccgctgggtc tgaaagcgga
     7561 taacctgcct tttcacggta cggatatctg gacgctgtat gaactttcct ggctgaatgc
     7621 gaaaggtttg ccgcaggtcg ctgtcggtca tgttgaactt gattacacca gcgtaaatct
     7681 gattgagtcg aagagtttta agctctatct caacagtttt aaccagacgc gttttaataa
     7741 ctgggatgag gtgcgccaga cgctggagcg cgacttaagc acttgcgctc agggtaagat
     7801 tagcgtggcg ttatatcgtc ttgatgaact ggaaggccag ccgataggtc atttcaatgg
     7861 cacttgcatt gatgaccagg atatcactat cgataactat gaattcacta ctgactatct
     7921 ggagaatgcc accagcggtg aaaaagtagt ggaagagacg ctcgtcagcc acctgctgaa
     7981 atcaaactgc ctgatcaccc atcaaccaga ttggggttcg atccaaattc agtatcgtgg
     8041 acgccaaatt gacagagaaa aactgctgcg ttacctggtc tcattccgtc atcacaacga
     8101 gttccacgaa cagtgcgtgg aacgcatctt taatgacctg ttacgcttct gccagccaga
     8161 gaaattgagc gtttacgcac gttatacccg tcgtggcggt ctggacatta acccgtggcg
     8221 cagtaatagc gattttgtcc catcgaccac aagactggtt cggcaataaa ttttttctca
     8281 attttgcgtg ctggattcac gcagaaggtt gtgaaaggtc atcaggcagg gctattgtaa
     8341 tcaaagggaa tgacgatatt cgtcccataa ggagtttttc ttgattacac atattagccc
     8401 gcttggctcc atggatatgt tgtcgcagct ggaagtggat atgcttaaac gcaccgccag
     8461 cagcgacctc tatcaactgt ttcgcaactg ttcacttgcc gtactgaact ccggtagttt
     8521 gaccgataac agcaaagaat tgctgtctcg ttttgaaaat ttcgatatta acgtcctgcg
     8581 ccgtgaacgc ggcgtaaagc tggaactgat taatcccccg gaagaggctt ttgtcgatgg
     8641 gcgaattatt cgcgctttgc aggccaactt gttcgcggtt ctgcgagaca ttctcttcgt
     8701 ttacgggcaa atccatcata ccgttcgttt tcccaacctg aatctcgaca actccgtcca
     8761 catcactaac ctggtctttt ccatcttgcg taacgctcgc gcgctgcatg tgggtgaagc
     8821 gccaaatatg gtggtctgct ggggcggtca ctcaattaac gaaaacgagt atttgtatgc
     8881 ccgtcgcgtc gggaaccagc tgggcctgcg tgagctgaat atctgcaccg gctgtggtcc
     8941 aggagcgatg gaagcgccga tgaaaggtgc tgcggtcgga cacgcgcagc agcgttacaa
     9001 agacagtcgt tttattggta tgacagagcc gtcgattatc gccgctgaac cgcctaaccc
     9061 actggtcaac gaattgatca tcatgccgga tatcgaaaaa cgtctggaag cgtttgtccg
     9121 tatcgctcac ggcatcatta tcttccctgg cggtgtgggt acggcagaag agttgctgta
     9181 tttgctggga attttaatga acccggccaa caaagatcag gttttaccat tgatcctcac
     9241 cggcccgaaa gagagcgccg actacttccg cgtactggac gagtttgtcg tacatacgct
     9301 gggcgaaaac gcgcgccgcc attaccgcat catcattgat gacgccgctg aagtcgctcg
     9361 tcagatgaaa aaatcgatgc cgctggtgaa agaaaatcgc cgtgatacag gcgatgccta
     9421 cagctttaac tggtcaatgc gcattgcgcc agatttgcaa atgccgtttg agccgtctca
     9481 cgagaatatg gctaatctga agctttaccc ggatcaacct gttgaagtgc tggctgccga
     9541 cctgcgccgt gcgttctccg gtattgtggc gggtaacgta aaagaagtcg gtattcgcgc
     9601 cattgaagag tttggtcctt acaaaatcaa cggcgataaa gagattatgc gtcgtatgga
     9661 cgacctgcta cagggttttg ttgcccagca tcgtatgaag ttgccaggct cagcctacat
     9721 cccttgctac gaaatctgca cgtaatctcc cctcccccgg tgtaatgccg atcagttaag
     9781 gatcagttga ccgatccagt ggctgtgtaa gaatccggaa acgctcactt gtttccggat
     9841 t
//
LOCUS       DAZPCS010000137         9826 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZPCS010000137 DAZPCS010000000
VERSION     DAZPCS010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9826
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.137"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            383..1501
                     /locus_tag="ACHUQ7_003179"
     CDS             383..1501
                     /locus_tag="ACHUQ7_003179"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFR1136471.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTPPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFAPSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            1567..1815
                     /locus_tag="ACHUQ7_003180"
     CDS             1567..1815
                     /locus_tag="ACHUQ7_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HFR1136472.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLHFIWRRWFSLAD"
     gene            1880..2248
                     /locus_tag="ACHUQ7_003181"
     CDS             1880..2248
                     /locus_tag="ACHUQ7_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFR1136473.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFCPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2342..2995
                     /gene="nfsB"
                     /locus_tag="ACHUQ7_003182"
     CDS             2342..2995
                     /gene="nfsB"
                     /locus_tag="ACHUQ7_003182"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFR1136474.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFAYMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            3103..4350
                     /gene="ybdG"
                     /locus_tag="ACHUQ7_003183"
     CDS             3103..4350
                     /gene="ybdG"
                     /locus_tag="ACHUQ7_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFR1136475.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4418..5794)
                     /gene="pheP"
                     /locus_tag="ACHUQ7_003184"
     CDS             complement(4418..5794)
                     /gene="pheP"
                     /locus_tag="ACHUQ7_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFR1136476.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGYGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(5895..9038)
                     /gene="cusA"
                     /locus_tag="ACHUQ7_003185"
     CDS             complement(5895..9038)
                     /gene="cusA"
                     /locus_tag="ACHUQ7_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFR1136477.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPKMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCAPFLWHVRSALVAIISLPLGLCIAFIVMPFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKQLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRN"
     gene            complement(9050..9568)
                     /gene="cusB"
                     /locus_tag="ACHUQ7_003186"
                     /pseudo
     CDS             complement(9050..9568)
                     /gene="cusB"
                     /locus_tag="ACHUQ7_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
BASE COUNT         2408 a         2721 c         2377 g         2320 t
ORIGIN      
        1 gttgatagaa atatctcctg gggcttcatc tttccagacc tcagagtaaa cctgaaatcg
       61 gaaagcttca ggcacccgca gttatcttac ccttcctcaa caaaatagct gtttatttat
      121 acagcaaatc ctgtttcgga cttctcactc cccatttgat caactgaagc tgaacccgtt
      181 tttttctgca tgcctcaatc gtcaaaattc ttcgccacca gccagctctg ccataacttg
      241 ccacattgaa aaagtagcca aagtgcttca ggcaccatca gttcaccatc accaaaaaaa
      301 agaaacattc cagaacttcc gcatctcgcc cagccgctcc gacttctata ctgaatagaa
      361 aacgtcaact taagagaaac ctatgccatt acccgatttt catgtttctg aaccttttac
      421 cctcggtatt gaactggaaa tgcaggtggt taatccgccg ggctatgact taagccagga
      481 ctcttcaatg ctgattgacg cggttaaaaa taagatcacg gccggagagg taaagcacga
      541 tatcaccgaa agtatgctgg agctggcgac ggatgtttgc cgtgatatca accaggctgc
      601 cgggcagttt tcagcgatgc agaaagtcgt attgcaggca gccgcagacc atcatctgga
      661 aatttgcggc ggtggcacgc ccccgtttca gaaatggcag cgtcaggagg tatgcgacaa
      721 cgaacgctat caacgaacgc tggaaaactt tggctatctc atccagcagg cgaccgtttt
      781 tggtcagcat gtccatgttg gctgtgccag tggcgatgac gccatttatt tgctgcacgg
      841 cttgtcacgg tttgtgccgc actttattgc cctttccgcc gcgtcgccat atatgcaggg
      901 aacggatacg cgttttgccc cctcacgacc gaatattttt tccgcctttc ctgataatgg
      961 cccgatgccg tgggtcagta actggcaaca atttgaagcc ctgtttcgct gtctgagtta
     1021 caccacgatg atcgacagca ttaaagatct gcactgggat attcgcccca gtcctcattt
     1081 tggcacggtg gaggttcggg tgatggatac cccgttaacc cttagccacg cggtaaatat
     1141 ggcgggatta attcaggcca ccgcccactg gttactgacg gaacgcccgt tcaaacatca
     1201 ggagaaagat tacctgctgt ataaattcaa ccgtttccag gcctgtcgct atggtctgga
     1261 aggcgtcatt accgatccgc acactggcga tcgtcgaccg ctaacggaag ataccttgcg
     1321 attgctggaa aaaatcgccc cttcagcaca taaaattggc gcatcgagcg caattgaagc
     1381 actgcatcgc caggtcgtca gcggtctgaa tgaagcgcag ctgatgcgcg atttcgtcgc
     1441 cgatggcggc tcgctgattg ggctggtgaa aaagcattgt gagatctggg ccggtgacta
     1501 agccgggaat tgccttacag aagaatatcc ctgacaatgg tgttttaact tacatttaac
     1561 aatgttatga aacacccttt agaaaccttg accaccgcag caggcatttt gctgatggct
     1621 ttcctctctt gcctgctgct gcccgccccc gcactggggc tgacgctggc acaaaaactg
     1681 gtgaccatgt tccatctgat ggatcttagc cagctttaca ctttattgtt ttgtctgtgg
     1741 tttttagtgc tgggcgctat tgagtatttt gttctgcact ttatctggcg acgctggttc
     1801 tcgctggcgg attaaacgtc gattatcgcc gaacttcgcg gcatactttg cttattctcc
     1861 ttcgcctaaa ggaacgttta tggataagca atcactgcac gaaacggcga aacgcctagc
     1921 tcttgagtta ccctttgtcg agctttgctg gcctttttgc ccggagttcg atgtttttaa
     1981 aattggcggc aagattttta tgctgtcgtc ggagctacgc ggcgtcccct ttatcaatct
     2041 gaagtccgat ccacaaaaat ccctgttaaa tcagcaaata tacccaagca ttaagccagg
     2101 gtatcacatg aataaaaagc actggatttc ggtgtattcc ggcgaggaaa tctccgaagc
     2161 gttacttcgc gatctgatca acgattcgtg gaatctggtg gttgatggtc tggctaaacg
     2221 cgatcaaaaa agagtgcgtc caggctaaag cgggaatcta tagcgcattt ttctcgctta
     2281 ccatttctcg ttgaaccttg taatctgctg gcacgcaaaa ttacttacac atggagtctt
     2341 tatggatatc atttctgtcg ccttaaagcg tcattccact aaggcatttg atgccagcaa
     2401 aaaacttacc ccggaacagg ccgagcagat caaaacgcta ctgcaataca gcccatccag
     2461 caccaactcc cagccgtggc attttattgt tgccagcacg gaagaaggta aagcgcgtgt
     2521 tgccaaatcc gctgccggta attatgtgtt caacaaacgt aaaatacttg atgcctcgca
     2581 cgtcgtggtg ttctgtgcaa aaaccgcgat ggacgatgcc tggctgaagc tggttgttga
     2641 ccaggaagat gccgatggcc gctttgccac gccggaagcg aaagccgcga acgataaagg
     2701 tcgcaagttc ttcgcctata tgcaccgtaa agatctgcat gatgatgcag agtggatggc
     2761 aaaacaggtt tatctcaacg tcggtaactt cctgctcggc gtggcggcgc tgggtctgga
     2821 cgcggtgccc atcgaaggtt ttgacgccgc aatcctcgat gcagaatttg gtctgaaaga
     2881 gaaaggctac accagtctgg tggttgttcc ggtaggtcat cacagcgttg aagattttaa
     2941 cgctacgctg ccgaaatctc gtctgccgca aaacaccacc ttaaccgaag tgtaattctc
     3001 tcttgccggg catctgcccg gctatttccc ctcagattct cctgatttgc ataaccctgt
     3061 ttcagccgtc atcataggct gctgttgtat aaaggagacg ttatgcagga tttaatatcc
     3121 caggttgaag atttagcggg tattgagatc gatcacacca cctcgatggt gatgattttc
     3181 ggtattattt ttctgaccgc cgtcgtggtg catattattt tgcattgggt ggtactgcgg
     3241 accttcgaaa aacgtgccat cgccagttca cggctttggt tgcaaatcat tacccagaat
     3301 aaactcttcc accgtttagc ttttaccctg caggggatta tcgtcaatat tcaggcggta
     3361 ttctggctgc aaaaaggcac cgaagcggca gatattctga ctacctgcgc gcagttgtgg
     3421 atcatgatgt atgcgctgct ttcagtcttc tcgttgctgg atgttatttt gaatctggcg
     3481 cagaaattcc cggcggcatc tcagttaccg ctgaaaggga tatttcaggg gattaaactg
     3541 atcggcgcaa tcctggtcgg cattttgatg atctcgctgc tgattggtca gtcgccagcg
     3601 attctaatca gcggtcttgg tgcaatggct gccgtgctga tgttggtatt taaagatccg
     3661 attcttggtc tggtggcagg tattcagctt tccgcgaacg atatgctgaa actgggcgac
     3721 tggctggaaa tgccgaaata cggcgcggat ggcgcggtga tcgatattgg gttaaccacc
     3781 gtcaaagtgc gtaactggga caataccatt accactattc ccacctggtc tctggtttct
     3841 gactccttta aaaactggag cgggatgtca gcatctggcg ggcgacgtat taagcgcagt
     3901 atcagtattg atgtcaccag tattcgtttt cttgatgaag acgaaatgca acgtctgaat
     3961 aaagcgcatt tgttaaagcc ttatttaacc agccgccatc aggaaattaa tgagtggaat
     4021 cgccagcaag gttctacgga gtcggtatta aacctgcgcc gaatgaccaa tattggaacc
     4081 tttcgtgcct atctgaacga atatctgcgt aaccatccgc ggattcgtaa agatatgacc
     4141 ttaatggtac gccaactggc tccaggtgat aacggtttac cgctcgagat ctacgcgttt
     4201 actaacaccg tggtgtggct ggaatatgaa agcattcagg ctgatatatt cgatcacata
     4261 tttgcgattg tcgaagagtt tggtctgcga cttcatcagt cgccaaccgg caatgatatt
     4321 cgctctctgg cgggtgcatt taagcagtaa ttaaaaaaac cgctctcatc gaatggatga
     4381 gagcggacat cgttttgtcg gatgtagcgt gaatgcctta tttccgacgc agcgttttaa
     4441 atgccacaaa caggaataca atccacaccg gcagcaggat cgctgacaag cgcatatcat
     4501 ccatcgtgca catcagcagc aaaatcatgc cgaggaaggc gatgcaaaga tagttgccga
     4561 acggatagag cagcgcctta aactgtgttt cacgcccctg acgtcgcatc gccgcgcgaa
     4621 aacgcagatg cgccaggcag atcataatcc agttcaacag caacgttgct accaccagcg
     4681 ccatcagcag accaaacgct ttttgcggca gcagatagtt gattaacacc accagcgaag
     4741 tgatcgctcc ggaaagcatc agcgagttaa tcggcacacc gcgacggctg acgcgagtca
     4801 aaaacttcgg cgcattaccc tgcacagaaa ggccaaacag catgcggctg ttagagtaaa
     4861 ccccgctgtt atacaccgac agcgatgcca ctagaatgac gaagttcagc gcagaagcta
     4921 ccacattgct gtcgagatta tggaaaatca tcacaaacgg gctactgttg gatttcactt
     4981 ccacccacgg atagagtgcc agtaaaacca ccagtgaacc gatataaaac agcaggatgc
     5041 gatacaccac ctgatttacc gcttttggaa tgcttttttc cggatcgcgc gcttcagcgg
     5101 ctgtaatccc aatcagctcc agaccgccga aggagaacat aattaccgcc agcgacaaaa
     5161 tcagcccatt ccagccggtg gcgaagaaac caccgtagcg ccagaggttg tcgatactgg
     5221 ctttctcgcc gccgtaacca gaaaacagca gccacaggcc aaagccgatc ataccgatga
     5281 tcgccagcac tttaatcagc gcaaaccaga actcggtttc gccatataag cgcacgttca
     5341 ccaggttaac ggcgttgatg ataataaaga aggcggcagc ccaaatccac gttggaacat
     5401 ccgggaacca gtactgcata tagatgcccg cagcggtcag ctctgccatt cccaccagca
     5461 cgaacattac ccagtagttc cagccagaga ggaagcccgc aaacggtccc cagtatttat
     5521 aggcaaagtg ggcaaatgaa ccggataccg gctcttcgac caccatctcg ccaagctggc
     5581 gcataatcag gaaagcgatg atcccggcga cgccgtagcc cagcaataca gccggacccg
     5641 ccatctgaat cgccgggcca atgccaagaa acagaccagt accaattgcg ccacccagcg
     5701 caatcagttg aatatgacgg ttatgtaatc cgcgatgaag cgtcggctct tgattcgacg
     5761 cagtatcttc cgatacggtt gacgcgtttt tcacgccttt cccctgtgtg tcttttttgt
     5821 tgaggggcac cttttaacat ttagtgccca tcgtcgcaag acacaatcca cacggttaaa
     5881 cgaggtatcc tgctttaatt ccgtacccga tgtcggtgca gccacatcag cttatacgcc
     5941 gccgggataa taaacagcga cagcaaaggt gcggtgatca tgccgccaat catcggtgcg
     6001 gcaatccggc tcatcacctc tgaaccagcc cccgttcccc acagaatcgg cagcagaccg
     6061 gcgataatca ccgccaccgt catcgctttc gggcgcacgc gcaggaccgc gccgtgatac
     6121 aacgcctcat ccagcttctg ctcgctgaat gtttgtggat tattcaacga cggctcggcc
     6181 tctatggcgt gacgtaaata catcagcatc accacgccaa attcggcggc gaccccggca
     6241 agggcaataa agccggtacc cgtcgccaca gaaagatgaa agcccatcca ccacagtaac
     6301 cagatgccgc ccaccagcgc aaacggtacg ctgctgataa tcagcaacgc ttcgcccacc
     6361 cgacggaatg ccagatacaa cagcacgaag atgatcatca gtgtcatcgg caccatgagt
     6421 ttcagcttat ggttggcgcg ctccagtagc tcgaactgcc cggagaatgc cacgctggtg
     6481 cccggtttta actgcacttt ttcagctatc gctttttgca aatcgtgaac caccgacacc
     6541 atgtcacgat cgcgggcatc gatataaatc cagctcgtcg ggcgcgcatt ctcggttttc
     6601 agcatcgacg gtccggtaga gactttaacg tcggccacgt ctgccagggt gatttgctgc
     6661 ttcatcggcg tcaggatcgg caactggcgc agtgcctgcg gactatcgcg ccagctttgc
     6721 ggataacgca gattaattgg ataacgggca atcccttcca ccgtttcgcc aaccatcgcc
     6781 ccgcccaccg cagaagtcac aaacaactgc acatccgcca ccgtcatacc gtaacgtgcg
     6841 gctttttcac ggttaatctc aacgttgata tagcgcccac cttccagccg ctcagcaagc
     6901 gcagaagcta cgcctggcac cgttcgcgcc acttcttcaa tttgctcagc catcgcgtcg
     6961 atatccgcca gcacagtgcc ggaaacttta atgccgatgg ggcttttaat gccggttgag
     7021 agcatatcga tacggttacg aattggcggc acccacagat tcgccagccc cggcagacgc
     7081 acggtgttat ccagttcctc aatgattttg tccattgtca tgcctggccg ccactgatcc
     7141 tgtggcttaa gctggatggt cgtttctacc atctccagcg gagcagaatc ggtggcggtt
     7201 tccgctttcc cggttttgcc aaatacccgc gccacttcag gtacgctcat aattagcttg
     7261 tcggtttttt gcagcatact cgccgcctct gctgcggaaa tccccggcag cgtcgatggc
     7321 atatacaaca agtcgccttc attgatctgc ggtaaaaatt ccccgccaac tttattgagc
     7381 ggccagagaa ccgtcagcac cgaaagcgcc gccaccagca gcgtggtttt cggccagtgc
     7441 agtactttca gcaacagcgg atgataaaca cgaatcaaaa agcgattgag cggattactg
     7501 ctttccggcg gaattttgcc acggatccag taacccatca ggatagggat cactacgatc
     7561 gccagcagcg ccgcacccgc catcgcatac gttttggtga acgccaacgg gccaaacaga
     7621 cgcccttcct gcccttccag ggtgaagatc gggataaacg acaacgtgat aatcagcaga
     7681 ctgataaaca gcgccggccc cacttcaaca gacgcatcgg tgatcacctg ccagcgcgtt
     7741 ttattatcca gcgtggcgtc aggatgctgg tgctgccact cttccagctg tttatgcgca
     7801 ttctcgatca tgacgatagc ggcatcgacc atcgccccga cggcaatcgc aatgccaccc
     7861 agcgacataa tattggcatt cagcccctgg aagggcatga caataaaagc aatacacaac
     7921 cccagcggca acgaaataat cgccaccagc gccgagcgca catgccagag aaacggcgcg
     7981 cagactaccg ccaccacaat aaactcttcc agcaacttgc cgctgaggtt gtcgatggcc
     8041 cggtcaatga gctggctgcg gtcgtatgtt gtaactatct ccacgccttc cggcagacta
     8101 cttttcagcg tttccagttt gtccttcacg gcggcgatca cttctcgggc gtttttgccg
     8161 gatcgcagga tcaccacccc gcccgccact tcgccttcgc cgtttagttc ggcaatgccc
     8221 cggcgcatct tcgggccaat ctggaccttc gcaacatcgc gcagataaac gggcacgcca
     8281 ttttcactgg cttttaaaac gatgtgatta aagtcgtcga gcgtttgtag atagccgctg
     8341 gcgcgcacca tatattctgc ttccgccagt tcgattgacg aaccgcccgc ttcctggttt
     8401 gaagcatcca gcgcgctttt tacttcggcg agactgatgc catactgcgc caggcgctga
     8461 ggatcgataa ccacctgata ctctttcacc acaccgccta ccgacgccac ttccgcaacg
     8521 tcaggaatgg ttttcagctc atatttgaga aaccagtcct gcaatgagcg taaatcggca
     8581 agatcgtgct taccgctgcg atccaccagt gcatattcat agatccagcc aacacccgtg
     8641 gcatctggtc ccagctcggc gctgactccc gcaggcagct taccctgtac ctggttgagg
     8701 tactccagca cccgcgaacg cgcccagtac ggatcggtgc catcttcgaa aatgacatac
     8761 acataagagt cgccaaactg cgagaaacca cgcacagtct tcgcgccagg caccgacaac
     8821 atggtggtgg ttagcggata agtcacctga ttttcaacga tttgcggtgc ctggccggga
     8881 tagctggttt taataatcac ctgcacatcg gagagatccg gcaacgcatc cactggcgta
     8941 ttaatgatgg tccaggtgcc ccagatgctc agaaacaacg cgcccatcag caccaaaaaa
     9001 cggttcgcca ccgagcgacg aataatccat tcaatcattg gttattccct caatgcgcat
     9061 gggtagcact ttcagagcgc atccgctcca gtgcgccaga aatattggct tcggaatcaa
     9121 tcaggaacag gccgccggaa accacctttt caccttccgc cagaccagag cgtaatgcgg
     9181 tgacgccttg cgatgcctgg aaaacagcaa cgcgtttcgg tacaaagcgc ccgtcggcat
     9241 caacggtaat cacccgctgt tcgttgccgg tatcaatcag cgcctgtgac ggaatgagca
     9301 gcatcggttc gctggcggtg ttgagttgca accaggcgtt catgcccggt tttagcgcct
     9361 cgtcggcgtt gtcgacttcc agacgcagct gcagcgtgcg ggtcgcggca tccacgccag
     9421 gtagcagcgt ccatttgcgg atggtgagtg ttttatccgg tcgcgccgga acggtgaggg
     9481 taaactgcga ggcatctttc accagccagg cgatggactc cgggatcgca gcagtgaccc
     9541 acaccgggtc cataccctga attcgcgatt acgcgcaatc cccctgagaa ccgccatgaa
     9601 caagggtttc atgatttgtg attttagttt ggtacgcaat ttggtacaca acactatttt
     9661 cacttcagcg ggtagtcatc aaactcacca taacgagcat cgttgatgat gtatgtgatc
     9721 accacaaata tcacatctgg actagttccg tcttcatgcg caggtagact ggccccctga
     9781 atctccagac aaccagtatc acttatttaa gtgatagtct taatac
//
LOCUS       DAZPCS010000138         9825 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZPCS010000138 DAZPCS010000000
VERSION     DAZPCS010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9825
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.138"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(77..421)
                     /locus_tag="ACHUQ7_003187"
     CDS             complement(77..421)
                     /locus_tag="ACHUQ7_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFR1136478.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            553..2463
                     /locus_tag="ACHUQ7_003188"
     CDS             553..2463
                     /locus_tag="ACHUQ7_003188"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFR1136479.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            2521..3216
                     /gene="tsaB"
                     /locus_tag="ACHUQ7_003189"
     CDS             2521..3216
                     /gene="tsaB"
                     /locus_tag="ACHUQ7_003189"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFR1136480.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            3256..3837
                     /gene="yeaY"
                     /locus_tag="ACHUQ7_003190"
     CDS             3256..3837
                     /gene="yeaY"
                     /locus_tag="ACHUQ7_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFR1136481.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSTTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            4043..5728
                     /gene="fadD"
                     /locus_tag="ACHUQ7_003191"
     CDS             4043..5728
                     /gene="fadD"
                     /locus_tag="ACHUQ7_003191"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFR1136482.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGIIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            5810..6925
                     /gene="rnd"
                     /locus_tag="ACHUQ7_003192"
     CDS             5810..6925
                     /gene="rnd"
                     /locus_tag="ACHUQ7_003192"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFR1136483.1"
                     /translation="MITTDDALASLCEAVRVFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(6979..7944)
                     /gene="yeaX"
                     /locus_tag="ACHUQ7_003193"
     CDS             complement(6979..7944)
                     /gene="yeaX"
                     /locus_tag="ACHUQ7_003193"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFR1136484.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIVGGIGITPFLSHMAELQHSDIDWQLHYCSRNPESCAFRAELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(8000..8617)
                     /gene="yeaW"
                     /locus_tag="ACHUQ7_003194"
                     /pseudo
     CDS             complement(8000..8617)
                     /gene="yeaW"
                     /locus_tag="ACHUQ7_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
     gene            complement(8959..9567)
                     /locus_tag="ACHUQ7_003195"
     CDS             complement(8959..9567)
                     /locus_tag="ACHUQ7_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR1136485.1"
                     /translation="MTGEKESKNAERIFMPPEIMRFKERLEEAMNGESSRAFAAKCGL
                     SDGVIRNYLSGKTYPSLDRLAQIAYATGRPIEWFIQSQSMPMVEHKNNQENKNSQPDR
                     STLKTKLIAIVELLEDKELHSAIELFRKKGFEALMPEIFSSDDSIQADLQGISQQTLQ
                     TAKMLESLPTDTRKKILSKYGIYEQEGLVAPSQEPQDVKKAV"
     gene            9737..>9825
                     /locus_tag="ACHUQ7_003196"
     CDS             9737..>9825
                     /locus_tag="ACHUQ7_003196"
                     /inference="COORDINATES: protein motif:HMM:NF025073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1136486.1"
                     /translation="MSAKNQDWHAEQIKAAVRMKGKTLSQLSR"
BASE COUNT         2378 a         2412 c         2585 g         2450 t
ORIGIN      
        1 ttctctggtc gatttatgta aaaccgacca gtattatcga taaattatcg tctgacttat
       61 tttgcttcga taaagcttac accgcagcca caatcttaat ttcaacttta tacttcgggt
      121 tcatcaaacc cgcttgtacc gtgcagcgca ccggcgcatg acccgcgaca acccaggcat
      181 cccacgcttt attcatcgcc gcgaagtcgt ttttatcggc caggaaaatg gtggcatcca
      241 gaatgctcga tttattgctg ccctgttttt ccagcacggc gtcaatctgt gccagcgtgt
      301 tggcggtttg ctcaaaggca tcggcgtcga ggttttccgg tacaccagtg tagtagagcg
      361 tgttgttgtg gattactaca tcagaccagc gggcttcagc atcgatacga acgatagtca
      421 tcattttgtc ctcattatac ttccattttc gtgctgcaaa gactgccaca atgtgaggtt
      481 gccgtcacca cttcaactgg cgaaagcgcc ctcatcctga cataatccct gttcaaatca
      541 acagggggta gtgtgacgga cgattttgca ccagacggtc agctggcgaa agcgatacca
      601 ggctttaagc cgcgagaacc acagcgacag atggcggtag ccgtcaccca ggcgatagaa
      661 aaaggccagc cgctggtggt ggaagcagga accggtacgg gcaaaaccta cgcttacctg
      721 gctcctgcgc tgcgggcgaa aaagaaagtc attatctcga ccggctcaaa agcgttgcag
      781 gatcagctct acagccgcga tttgccaaca gtctcaaagg cattgaaata tacgggcaac
      841 gtggcgctgc tgaaagggcg ctcaaactac ctctgcctcg aacgtctcga acagcaggcg
      901 ctggcggggg gcgatctgcc ggtacaaatc ttaagcgatg tgatcctgct gcgctcctgg
      961 tctaatcaaa cagtcgatgg tgatatcagc acctgcgtca gcgtggcgga agattcacag
     1021 gcgtggccgc tggtcaccag caccaacgac aactgtcttg gcagcgactg cccgatgtat
     1081 aaagattgct ttgtggtcaa agcacgtaaa aaagcgatgg acgccgatgt ggtggtggta
     1141 aaccatcatc tctttctggc ggatatggtg gttaaagaga gtggatttgg cgagttgatc
     1201 cctgaagcgg acgtcatgat cttcgacgaa gcccaccagc taccggacat tgccagccag
     1261 tattttggtc agtcactctc cagtcgacaa ctgctcgacc tggcaaaaga catcaccatc
     1321 gcctaccgca ccgaattaaa agacacccag cagttacaaa agtgcgctga tcgtcttgcc
     1381 cagagtgcgc aggattttcg tctgcaactc ggtgagccag gttatcgcgg taacctgcgt
     1441 gagctgttag ctaatccgca aattcagcgg gcatttttac tgctcgatga caccttggaa
     1501 ctttgttatg atgtggcgaa actgtcactg gggcgttccg ccttgctgga tgcggcattt
     1561 gagcgcgcca cgttgtatcg cacacggctg aagcggctaa aagagatcaa tcagccgggc
     1621 tacagctact ggtacgaatg cacttcgcgc cattttactc tggctctcac gccgctcagc
     1681 gtggcggata aattcaaaga gttaatggcg caaaagcccg gtagctggat cttcacctca
     1741 gcaacgctgt cggtgaacga cgatctgcat catttcacct cgcggcttgg gatagaacag
     1801 gcggagtcgt tgctattgcc aagcccgttt gattacagcc gccaggcgtt actctgtgtg
     1861 ccgcgcaatc tgccgcaaac caaccagcca ggttctgctc gccagttagc ggcaatgctg
     1921 cgaccgatca tcgaagctaa caacggtcgt tgttttatgc tttgtacctc gcacgccatg
     1981 atgcgcgatc tggccgagca gttccgcgct accatgacgc ttcccgtttt gttgcagggg
     2041 gaaaccagta aaggccaact gttgcagcag tttgtcagtg ccggtaatgc gcttcttgtg
     2101 gcaaccagta gtttctggga aggggtggac gtgcgtggcg atacattgtc attggtaatt
     2161 atcgacaaat tgccgtttac ctcgccggat gatccactgt taaaagcgcg catggaagat
     2221 tgtcgtttgc gcggtggcga cccgttcgat gaagtgcaac taccagatgc cgtcattact
     2281 ctcaaacagg gggtaggtcg actgattcgc gacgccgacg atcgtggcgt gctggtgatt
     2341 tgtgactatc ggctggtgat gcgtccttac ggcgcgacgt ttctcgccag tctgccgccc
     2401 gcgccacgca cccgtgacat tgcccgtgcg gttcgtttcc ttgcgatacc atcctccagg
     2461 taatttgtta cggagtatgg tatgatgcgc gcctttttat tgatctaagc acgaagaccc
     2521 atgcgaattc tggctatcga taccgcgaca gaggcctgct ctgtcgccct gtggaacgac
     2581 ggtactgtca acgctcattt tgagctttgc cctcgtgaac atactcaacg aatcttaccg
     2641 atggtgcagg atatcctgac caccagcgga acttccctga ctgatattaa cgctctggct
     2701 tacgggcgcg gccccggtag ctttaccggc gtgcgcattg gtattggcat cgcgcaaggg
     2761 ctggcgcttg gcgcggaatt accgatgatt ggcgtctcca cgctaatgac gatggcgcaa
     2821 ggggcgtggc gcaaaaacgg cgcaacacgc gtgctgtcag ccattgatgc gcgaatgggc
     2881 gaagtttact gggccgaata tcagcgtgat gaaaacggta tctggcacgg tgaagaaacc
     2941 gaagccgtac tcaaacccga actcgttcat gaacgaatgc aacagctttc cggcgaatgg
     3001 gtgacggtag ggacgggctg gcaagcctgg ccggatctcg gtaaagagag cgggctggtt
     3061 ttgcgcgatg gcgaagtgtt actccccgct gcagaagata tgctgccgat tgcttgtcag
     3121 atgtttgccg agggtaaaac ggtggcggtg gaacatgccg agccggttta tttgcgtaac
     3181 aacgtcgcat ggaagaaact tccgggcaaa gaatgaatct taagagtatg ccctgagaaa
     3241 aaaaggagtc gcatcatggc ggttcaaaag aatgttatca aaggcatact ggcaggtacg
     3301 tttgcgctaa tgctgagcgg ttgtgtcact gtgccggacg ccattaaagg cagcagtacc
     3361 acgccgcaac aagatttagt tcgggtgatg agtgcgccgc agctgtacgt tggtcaggag
     3421 gcacgctttg gtggcaaagt ggttgcggta caaaaccagc aagggaaaac ccgcctggaa
     3481 attgctaccg taccgctgga cagcggagct aggccgacgc tgggagaacc ttctcgcggt
     3541 cgcatttatg ccgatgtgaa cggttttctg gacccggtgg atttccgtgg acaactggtt
     3601 acggtagtcg ggccaatcac cggtgcggtt gacggcaaaa tcggcaatac gccctataaa
     3661 tttatggtga tgcaagtaac gggttacaaa cgttggcatt taacccagca ggtgattatg
     3721 ccgcctcagc cgattgatcc atggttttat ggcggtcgtg gctggcccta tggctacggc
     3781 ggatggggct ggtataatcc cggccccgcg agagtacaaa cagttgtaac cgaataattg
     3841 cttgttttta aagaatgcag aaacagcggc tggtccgctg tttctgcatt cttacggtaa
     3901 agataaaaat aaatagtgac gcgcttcgca accttttcgt tgggtaatta tcaagctggt
     3961 atgatgagtt aatattatgt taacggcgtg tatatcattt ggggttgcga tgacgacgaa
     4021 cacgcatttt agaggtgaag aattgaagaa ggtttggctt aaccgttatc ccgcggacgt
     4081 tccgacggag atcaaccctg accgttatca atctctggta gatatgtttg agcagtcggt
     4141 cgcgcgctac gccgatcagc ctgcgtttgt gaatatgggg gaggtgatga ccttccgcaa
     4201 gctggaagaa cgcagtcgcg cgtttgccgc ttatttgcaa caagggttgg ggctgaagaa
     4261 aggcgatcgc gttgcgttga tgatgcctaa cttattgcaa tatccggtgg cgctatttgg
     4321 cattttgcgt gccgggataa tcgtcgtaaa cgttaacccg ttgtataccc cgcgtgagct
     4381 tgagcatcag cttaacgata gcggcgcatc ggcgattgtt atcgtgtcta actttgccca
     4441 cactctggaa aaagtggttg ataaaaccgc cgttcagcac gtaattctga cccgtatggg
     4501 cgatcagcta tctacggcaa aaggcacggt agtcaatttc gttgttaaat acatcaagcg
     4561 cttggtgccg aaataccatc tgccagatgc catttcattt cgtagcgcac tgcataacgg
     4621 ctaccggatg cagtacgtca aacccgaact ggtgccggaa gatttagcct tcctgcaata
     4681 caccggcggc accacaggtg tggcgaaagg cgcgatgctg actcaccgca atatgctggc
     4741 gaacctggaa caggtcaacg ccacctatgg gccgttgctg catccaggta aagaactggt
     4801 agtaacggca ctgccgctgt atcacatttt tgccctgacc attaactgcc tgctgtttat
     4861 cgaactgggt gggcagaacc tgcttatcac taacccgcgt gatattccag ggttggtaaa
     4921 agagttagcg aaatatccgt ttaccgctat cactggcgtt aacaccttgt tcaatgcgtt
     4981 gctgaacaat aaagagttcc agcagctgga tttctccagt ctgcatcttt ccgcaggcgg
     5041 tgggatgcct gttcagcaag tggtggcaga acgctgggtg aaactgaccg gacagtatct
     5101 gctggaaggt tatggcctga ccgagtgcgc gccgctggtg agcgttaacc cgtatgatat
     5161 tgattatcat agcggtagca tcggtttgcc ggtgccatcg acggaagcca aactggtgga
     5221 tgacgacgac aacgaagtac caccgggtca accgggtgag ctttgtgtca aaggaccgca
     5281 ggtgatgctg ggttactggc agcgtccgga tgctacagat gagatcatca aaaatggctg
     5341 gttacacacc ggcgacatcg cggtgatgga tgaagaagga ttcctgcgca ttgtcgatcg
     5401 taaaaaagac atgattttgg tttccggttt taacgtttat cccaacgaga ttgaagatgt
     5461 cgtcatgcag catcctggcg tacaggaagt cgcggctgtt ggcgtacctt ccggctccag
     5521 tggtgaagcg gtgaaaatct tcgtagtgaa aaaagatcca tcgcttaccg aagagtcact
     5581 ggtgactttt tgccgccgtc agctcacggg atacaaagta ccgaagctgg tggagtttcg
     5641 tgatgagtta ccgaaatcta acgtcggaaa aattttgcga cgagaattac gtgacgaagc
     5701 gcgcggcaaa gtggacaata aagcctgagc gttaagtcag tcgtcagacg ccggttaatc
     5761 cggcgttttt tttgacgccc actaaagaga aaacaatttg aattaccaaa tgattaccac
     5821 ggacgatgcg ctggcttctt tgtgtgaagc cgtccgtgtc tttccggcga tagccctgga
     5881 tactgaattt gttcgtacgc gcacttatta cccgcagctg gggttgattc aacttttcga
     5941 tggcgagcat ctggcgctaa ttgatcctct cgggatcacc gactggtcac cgctgaaagc
     6001 gatcctgcgc gatccgtcca tcacaaaatt tctccatgca ggcagtgaag atctggaagt
     6061 gttcctcaat gtctttggcg aattaccaca acccttgatt gacacgcaaa tccttgctgc
     6121 cttctgcgga cgcccgatgt catggggttt cgcttccatg gtggaagagt attccggcgt
     6181 tacgctggac aagagtgaat cgcgcaccga ctggctggcc agaccgctga ccgaacgtca
     6241 gtgtgaatac gcagcggcgg atgtctggta tctgttaccg atcaccgcca agcttatggt
     6301 agaaacggag gcctccggct ggctacctgc ggcgctggat gaatgccgcc tgatgcaaat
     6361 gcgtcgtcag gaagtcgttg cgccggaaga tgcctggcgt gatatcacca atgcctggca
     6421 gttacgcaca cgccaactgg cctgtctgca actgttagcc gactggcgac tgcgcaaggc
     6481 gcgagagcgt gatctggcgg tgaactttgt cgtgcgtgaa gagcatttgt ggtcggtcgc
     6541 gcgttatatg ccgggaagtt taggcgaact ggacagcctg ggtttatccg gtagcgaaat
     6601 ccgctttcac ggtaaaacgc tgctggcgct ggtggaaaaa gcgcagacat tgccggaaga
     6661 tgccttaccg cagccgatgc ttaacctgat ggacatgcct ggttatcgta aagcgtttaa
     6721 agcgattaag tcgctgatta ctgacgtgag cgaaacgcat aagatcagcg ccgaattgct
     6781 ggcatcgcgt cggcaaatca accaactgct gaactggcac tggaaactga aaccgcagaa
     6841 caatttgccg gagctgattt ccggctggcg tggtgagctg atggcggaag cattacacaa
     6901 tttattgcag gaatatccgc agtaaaatct tccgaagccg gactgggcgc gctcagcgcc
     6961 acatccggct ttggcaaact acaaatccaa caccaggcgt ttacccttcg cacgcgaaca
     7021 acagatcaac atactttgct ggctggcacg ctcttcatcg ctaaaatact gatcccgatg
     7081 gtcagcttca ccttccagta ttgccgtttc gcaggttccg catacccctt cacgacataa
     7141 acattccact ttcgcggctt tattattttc aataacctgc aaaatagtca tctcttccgg
     7201 caccacaaac tcttttccgg aacgggcaag caccagggta aatgcatcgc cggttttgtc
     7261 ttcgatagca aattgctcaa agtgcagtgt atcggcggcg atgtccagac gcgcagcttc
     7321 acttcttacc gcttcaatta gcgcctcggg gccacaggta taaacgtgtg tgccaggttc
     7381 gatatccgcc aataatcgcg ctaattccag tcgtgttccg gttgatgaat gatgcaaatg
     7441 gactttctca gcctgcggat gctggactag ctcagcacga aatgcgcaac tttctggatt
     7501 tcgcgagcag taatgtagct gccagtcgat gtcgctgtgt tgcaactctg ccatgtgcga
     7561 caggaaaggg gtgataccaa taccgcccac gataaacaga tgctttctgg ctgagggaat
     7621 tagcgcaaac aggttattag gcgttgagat cgttaaccga tcgcccactt ttacctgctg
     7681 atgcaaaaag cgggaaccgc cgcgcgagtt ttcttccagt cgaacggcaa tctgataact
     7741 agaggtgtta tgcggtgaac tcagtagtga atacgcattg ctgtactggt tatcaccatc
     7801 gctcatctgc actaggatgt gacttcctcc ggtgaacgca ggcagcggtt tgccatcggt
     7861 tgccaccagc gtgaagcgtt tcacctgttc ggtaaggggt tcaacctggc tcacctgtac
     7921 ttcaaacatt tgatagtctg acattgctgt actccatcaa aaatcagccc agcggcggaa
     7981 aataccgccg ccgatgtcat tagtccttaa acacctgcgc cagcagatta tggaaatggg
     8041 cgataccatg ttcggaaatg ccactaccgc tactgtcggc catgatgcgc ccctgaccac
     8101 gatagccacg cgatttcagc cctttctgta cgctttcaac cagacgtaaa tcttccggat
     8161 ggaacacatc gcgataccac tcaatcagcg atttttgctc gtcggttaac tcttcattgg
     8221 tgaagtaaat atcgtagttt tgcagggtag tttcagaatc caccgggaat tcataaatga
     8281 ccgtcatcat ccctttgatc ggggtgacgt tcagcatcgt gcacggccac agccagaaac
     8341 cgtggaatgc cgcatccgta ccctcttcaa atttaaacga ctgttcggac ggtttggcga
     8401 aaccgtattg cagcgtccag ttaccgtgca tggtgtgcca gtaacgatca acctgtacgg
     8461 agtcggagaa acctagatgc gccggaccac agtgatagca ctcgagatag ttatcgacaa
     8521 tgttcttcca gttggcaggc gtgcgggtgg taaagcgggc cgctagtttc agattgtgga
     8581 cttccgggca ggcttccagc actttcgcgc ccagaccggt gtattgattc acttgaagta
     8641 cgaaaaaaaa ccgggaggac attggattat tcgggttctg atgggattag atttggtggg
     8701 gcttgcaagc ctgtagcgca aattttagtc gttaatcaat gaaacgcgaa agatagtaaa
     8761 aaattgcttt tgtttcattg aaaatacgaa aaacaaaaac actgcaaatc atttcaataa
     8821 cagcttcaaa aaacgttcaa aaccgataac aaccaggctg taaccaaatg actcatatca
     8881 caaatcagct catgccgttt aggtatgtta catgtgtgat tatgtgaggt gaagtatgtt
     8941 ttagctggtt catggttgtt atacggcttt ttttacatct tgtggttctt gtgaaggagc
     9001 tacaagacct tcctgttcat aaataccata ctttgacaaa atctttttac gagtatctgt
     9061 tggtaatgat tcaagcattt tggctgtttg taaggtttgc tgcgaaatac cttgcaaatc
     9121 agcctggatg gaatcatcgg aagaaaaaat ctcaggcata agagcttcaa atcctttctt
     9181 tctgaacaac tcaatcgcag aatgcagttc cttgtcctct aacaattcga caattgcgat
     9241 cagtttagtt ttcaatgtgc tgcggtcagg ttgagaattt ttattctctt gattattttt
     9301 atgctcaacc attggcatgc tctgactttg gataaaccac tctattggac gccctgttgc
     9361 gtaggctatt tgtgctaatc gatctaaaga cgggtatgtc ttgccagaca ggtagtttct
     9421 gataacccca tcagaaagtc cgcacttagc tgcaaacgcc cggcttgatt ctccattcat
     9481 tgcctcttca aggcgttcct tgaaacgcat tatttccggg ggcataaaaa tgcgctctgc
     9541 atttttgctt tctttttcac ctgtcatgcc caatctctct gttatataaa gaattttaca
     9601 gaattctttg atttgcactg gttaaaataa aaatgcgcat agcttttgaa ttgagcattt
     9661 ttctgcgcta tcgtttttcc cgatagcggt agccggtatc agttaccgaa aacgttaaga
     9721 gagtaaggat cgcagaatgt cagcaaaaaa ccaagactgg cacgcagagc agataaaggc
     9781 tgctgttcgt atgaagggga agacactatc tcaattatcc agaga
//
LOCUS       DAZPCS010000139         9774 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZPCS010000139 DAZPCS010000000
VERSION     DAZPCS010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9774
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.139"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            392..2023
                     /gene="oppA"
                     /locus_tag="ACHUQ7_003197"
     CDS             392..2023
                     /gene="oppA"
                     /locus_tag="ACHUQ7_003197"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFR1136487.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            2109..3029
                     /gene="oppB"
                     /locus_tag="ACHUQ7_003198"
     CDS             2109..3029
                     /gene="oppB"
                     /locus_tag="ACHUQ7_003198"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFR1136488.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            3044..3952
                     /gene="oppC"
                     /locus_tag="ACHUQ7_003199"
     CDS             3044..3952
                     /gene="oppC"
                     /locus_tag="ACHUQ7_003199"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFR1136489.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            3964..4977
                     /gene="oppD"
                     /locus_tag="ACHUQ7_003200"
     CDS             3964..4977
                     /gene="oppD"
                     /locus_tag="ACHUQ7_003200"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFR1136490.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            4974..5978
                     /gene="oppF"
                     /locus_tag="ACHUQ7_003201"
     CDS             4974..5978
                     /gene="oppF"
                     /locus_tag="ACHUQ7_003201"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFR1136491.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(6031..6360)
                     /locus_tag="ACHUQ7_003202"
     CDS             complement(6031..6360)
                     /locus_tag="ACHUQ7_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFR1136492.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEISDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6395..7855)
                     /gene="cls"
                     /locus_tag="ACHUQ7_003203"
     CDS             complement(6395..7855)
                     /gene="cls"
                     /locus_tag="ACHUQ7_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFR1136493.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAH
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            7998..8171
                     /locus_tag="ACHUQ7_003204"
     CDS             7998..8171
                     /locus_tag="ACHUQ7_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFR1136494.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8226..9479)
                     /gene="kch"
                     /locus_tag="ACHUQ7_003205"
     CDS             complement(8226..9479)
                     /gene="kch"
                     /locus_tag="ACHUQ7_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFR1136495.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
BASE COUNT         2563 a         2286 c         2433 g         2492 t
ORIGIN      
        1 tgaaagatgt ttgttaaggc atggatgcaa gctatagatt ctgatacggt caataaaaga
       61 gaatcgctta acaattttgc aaaatgtatt ggcgagtaag aaccgcattt ggtactttcc
      121 gggcaaccgc cagacgattc tttattggta atgagaataa ttaacaatta aagagcgtcg
      181 cgaaagaata atgtgtctcg acaggggaga cacagtacga atcgacataa ggtgatcgtc
      241 tgaatcacca gaataaataa agtcggtgat agtaatacgt aacgataaag taacctgaca
      301 gcagaaagtc tccgagcttg tgcagggtcc caatccggga ttacacatgc tggttaatac
      361 cagtaattat aatgagggag tccaaaaaac aatgaccaac atcaccaaga gaagtttagt
      421 agcagctggc gttctggctg cgctaatggc agggaatgtc gcgctggcag ctgatgtacc
      481 cgcaggcgtc acactggcgg aaaaacaaac actggtacgt aacaatggtt cagaagttca
      541 gtcattagat ccgcacaaaa ttgaaggtgt tccggagtct aatatcagcc gagacctgtt
      601 tgaaggctta ctggtcagcg atcttgacgg tcatccagca cctggcgtcg ctgaatcctg
      661 ggataataaa gacgcgaaag tctggacctt ccatttgcgt aaagatgcga aatggtctga
      721 tggcacgcca gtcacagcac aagactttgt gtatagctgg caacgttctg ttgatccgaa
      781 cactgcttct ccgtatgcca gttatctgca atatgggcat atcgccggta ttgatgaaat
      841 tcttgaaggg aaaaaaccga ttaccgatct cggcgtgaaa gctattgatg atcacacatt
      901 agaagtcacc ttaagtgaac ccgttccgta cttctataaa ttacttgttc acccatcaac
      961 ttcaccggtg ccaaaagccg ctatcgagaa attcggcgaa aaatggaccc agcctggtaa
     1021 tatcgtcacc aacggtgcct ataccttaaa agattgggtc gtaaacgaac gaatcgttct
     1081 tgaacgcagc ccgacctact ggaacaacgc gaaaaccgtt attaaccagg taacctattt
     1141 gcctattgct tctgaagtta ccgatgtcaa ccgctaccgt agtggtgaaa tcgacatgac
     1201 gtataacaac atgccgatcg aattgttcca gaagctgaaa aaagagatcc cggacgaagt
     1261 tcacgttgat ccatacctgt gcacttacta ttacgaaatt aacaaccaga aaccgccatt
     1321 caacgatgtg cgtgtgcgta ccgcactgaa actaggtatg gaccgcgata tcattgttaa
     1381 taaagtgaaa gcgcagggcg acatgcccgc ctatggttat actccaccgt atactgatgg
     1441 cgcaaaattg actcagccag aatggtttgg ctggagccag gaaaaacgta acgaagaagc
     1501 gaaaaaactg ctggctgaag cgggttatac cgcagacaaa ccgttgacca tcaacctgcg
     1561 gtataacacc tccgatctgc ataaaaagct ggcgattgct gcctcttcat tgtggaagaa
     1621 aaacattggt gtaaacgtca aactggttaa ccaggagtgg aaaacgttcc tcgacacccg
     1681 tcaccagggc acttttgatg tggcccgtgc aggctggtgt gcagactaca acgaaccaac
     1741 ttccttcctg aacaccatgc tgtcgaacag ctcgatgaat accgcgcatt ataagagccc
     1801 ggcctttgac agcattatgg cggaaacgct gaaagtgact gacgaggcgc agcgcacagc
     1861 tctgtacact aaagcagaac aacagctgga taaggattcg gccattgttc ctgtttatta
     1921 ctacgtgaat gcgcgtctgg tgaaaccgtg ggttggtggc tataccggca aagatccgct
     1981 ggataatacc tatacccgga atatgtacat tgtgaagcac taatggcaat acgtggggca
     2041 ggagtgtcct gctccacggt gtctgatttt tatcgcatta cagaaggcac aggccagaag
     2101 gtagggcaat gttaaaattt attctacgtc gttgtctgga agcgattccg acgctattta
     2161 ttcttattac tatttcgttc tttatgatgc gcctcgcacc gggaagccct tttaccggcg
     2221 aacgtacttt accgccagaa gtgatggcca atatcgaagc gaaatatcat cttaatgatc
     2281 cgatcatgac acagtatttc agctacctga aacaactggc gcatggcgat ttcggtccat
     2341 cgtttaaata taaagattat tcggtcaatg acctggtggc atccagtttt cccgtttctg
     2401 ccaaactggg agccgccgca tttttccttg cggtaatact gggtgttagt gctggcgtta
     2461 ttgccgcatt aaaacaaaac accaaatggg actataccgt gatggggctg gcaatgaccg
     2521 gggttgttat ccccagtttt gtggttgcgc cattattagt catgatattt gcgatcattt
     2581 tgcattggct gccgggcggt ggctggaatg gtggggcgct taaattcatg atattgccga
     2641 tggtggcgtt gtcactcgcc tatatcgcca gtattgcgcg tattacccgt ggctctatga
     2701 ttgaagtatt acactccaac tttattcgta ctgcccgggc gaaagggtta cctatgcggc
     2761 ggatcatttt acgccatgca ttaaaacctg ctctgttacc cgtgctctcc tatatgggcc
     2821 ccgcatttgt cggcattatt accggttcta tggtcatcga aaccatttat ggtttgccgg
     2881 ggattgggca actgttcgtt aatggcgcat tgaaccgtga ctattcctta gtgttaagcc
     2941 tgaccatcct ggttggtgcg ttaaccattt tgtttaatgc cattgtcgat gtgctatatg
     3001 cggttatcga cccgaaaatc cgttactgat actggagctc gcgatgatgt taagtaagaa
     3061 aaacagcgag acgctggaaa atttcagtga aaagctggag gtcgaagggc gcagcttgtg
     3121 gcaggacgca cgtcgacgtt ttatgcataa ccgtgcggcg gttgccagtc tgatagtgct
     3181 ggtgctgatc gcgttatttg taatcctggc accgatgctt tcccagtttg cctatgacga
     3241 tactgactgg gcgatgatgt ccagcgcccc ggatatggag tccggtcact actttggtac
     3301 tgactcatcc ggtcgcgacc tgcttgtgcg cgttgcgatt ggcgggcgta tctcactcat
     3361 ggtcggtgtt gctgcggcac tggtggcagt ggtcgtgggg acactttatg gttcgctttc
     3421 cggttatctg ggcggtaaag tggattcggt aatgatgcgt ctgctggaaa tcctcaactc
     3481 cttcccattc atgttcttcg tcattttgct ggtgaccttt ttcggccaaa acatcctgct
     3541 gattttcgtg gcgattggca tggtttcctg gctggatatg gctcgtattg tgcgtgggca
     3601 aaccctgagt ctgaagcgca aagagtttat tgaggcggca caagttggcg gtgtatcgac
     3661 gccgggcatt gttattcgcc acattgtgcc gaacgtactc ggtgtggtgg tggtctacgc
     3721 atcgctactg gtgcccagca tgatcctctt tgaatctttc cttagcttcc tggggttggg
     3781 tacgcaagag ccgttaagca gctggggggc attgctgagt gatggcgcga actcgatgga
     3841 agtctctcca tggttactgt tgttcccagc gggattcctt gtggtgacac tgttttgttt
     3901 caactttatc ggcgatggct tgcgtgatgc cctcgacccg aaagatcgtt aaggagtgca
     3961 gccatgagcg taattgaaac tgcaactgtg ccgctcgcac aacaacaggc tgacgcactg
     4021 ctgaacgtga aagatttgcg tgtcaccttt agtaccccgg acggcgacgt cacggcggtc
     4081 aatgatttaa atttttccct acgtgccgga gaaacgctgg gcattgtagg tgagtctggt
     4141 tcgggtaaat cgcaaactgc atttgcgttg atgggcctgt tggctgccaa cggacgcatt
     4201 ggcggatcgg caaccttcaa tgggcgtgaa atcctcaatt tgccagagcg tgaactcaat
     4261 aaactgcgcg ctgaacaaat ctcaatgatt tttcaggacc caatgacttc gttgaatccc
     4321 tatatgcgcg tcggtgagca gttgatggaa gtgctgatgc tgcataagaa catgagcaaa
     4381 gctgaggcgt ttgaagagtc ggtgcggatg ctcgatgcgg taaaaatgcc ggaagcacgt
     4441 aaacgcatga aaatgtaccc gcacgaattt tctggcggca tgcgtcagcg agtcatgatt
     4501 gcgatggcat tgctatgtcg acctaagttg ctgattgcgg atgaacccac tacagcgctg
     4561 gacgtcaccg tacaggcgca gatcatgacg ctattgaatg aactgaagcg ggaatttaat
     4621 actgccatca ttatgatcac ccacgatctt ggcgtggtgg cggggatctg tgacaaagtg
     4681 ctggtaatgt acgccgggcg cacgatggaa tatggcaatg cgcgcgatgt cttttatcaa
     4741 cccgttcatc cttattctat cggtttgctc aacgcggtgc cgcgtcttga tgcggaaggt
     4801 gaaacaatgt tgaccatccc gggtaatccg ccaaacttgc tgcgattacc gaaaggttgc
     4861 ccgttccagc cacgttgtcc acatgcgatg gaaatttgta gtagcgcacc gccgctggaa
     4921 gagtttacgc ctggccgtct gcgtgcttgc tttaaaccgg tggaggaact gttatgaatg
     4981 ctgtaactga aggaagaaaa gtcctccttg aaatcgccga tcttaaagtg cactttgaaa
     5041 tcaaagatgg caaacagtgg ttctggcaac cgccgaaaac gctcaaagcc gtcgatggtg
     5101 tcactcttcg cctgtatgaa ggggaaacat taggtgtggt aggggaatcg ggatgcggta
     5161 agtccacctt tgctcgcgcc atcatcggtt tggtcaaggc gaccgacggt catgttgcct
     5221 ggttaggtaa agagttgctg ggcatgaagc ccgatgaatg gcgtgccgtt cgcagtgata
     5281 ttcagatgat tttccaggat ccgttggcat cgctaaaccc gcgtatgacc atcggcgaga
     5341 tcatcgctga accactgcgt acttatcatc cgaaaatgtc acgccaggaa gttcgcgagc
     5401 gcgtgaaggc gatgatgctg aaagtcgggt tattgcctaa cctgattaac cgctatccgc
     5461 atgagttctc cggtgggcag tgccagcgta tcgggattgc tcgtgctctt attcttgaac
     5521 cgaagctgat tatctgcgat gagccggttt cggcgctgga cgtgtcaatt caggcgcagg
     5581 tggttaacct gctccagcag ctgcaacgtg agatgggatt gtcattaatt tttattgctc
     5641 atgacctggc cgtggtaaaa cacatttccg atcgtgtgtt ggtgatgtat ctcggccatg
     5701 cagtagaact ggggacctat gatgaggtct accacaatcc actacatcct tacaccaggg
     5761 cattgatgtc ggcagtcccc atacctgatc cggatctgga gaagaacaaa accatccagt
     5821 tactggaagg ggaattaccg tcgccgatca acccgccttc cggttgtgtt ttccgtaccc
     5881 gttgcccgat tgccggtccg gagtgcgcca aaacacgtcc tgttctggag gggagtttca
     5941 gacacgccgt ttcttgcctg aaagtcgatc cgctttaaaa acaataaggg ctgacagttg
     6001 tcagcccttt ttcacgctaa aagcgattat ttattcccgc cagatgatat ggcaaagttt
     6061 gtgatctttt tcgcggcata acagaatgcg ggcgaaaaca tcgctgattt caccatcttc
     6121 actgtccgcc agaccaatca ccacttcggc aaaaaagtcc gggttcaaat cgaaatcaac
     6181 atgttcctgc cagtcttccg ccggatcgaa taactccgcg ccgccgcgct cttcaaactg
     6241 aagattgaac agcagaacat cagctggatc gagattgtcg gcagccagtt cgagaaaaat
     6301 atcgtaggcc tgctcaagcg tttcatcttc agtcaggcga ttgtttagat ccatatccat
     6361 agtcactacc tgtttaacct ctgttggcga cgttttacag caacgggctg aagaagtaaa
     6421 acagtcgctc ggcgacacgt tgccataatg gacgttttag ccataaacgg gcatcgagca
     6481 gacgtgaacg cgaaatataa tcgtcctgaa cggcggcaag gtctgcacca aaacctttat
     6541 cgtcgattgc caaggtaatc tcgaaattca gccacagact acgcatatca aggttaacgg
     6601 tgccaaccag acttagttcg ccatcgacca gcacgctctt ggtatgcagt aacccgcctt
     6661 caaactgata aattttaacc ccagcagcaa gcagttccgt aaagaatgcg cgactggccc
     6721 agccgaccag catcgagtca ttttttcgcg gaaggataat actgacatcc accccgcgct
     6781 gcgccgccgt gcaaatcgca tgaagtaaat catcgcttgg cacaaagtag ggcgtggtca
     6841 tgatcaaata ttcatgcgcc gaataagccg cagtcaataa tgcctggtga atgagatctt
     6901 ccggaaagcc ggggccagaa gcaattgtgt gaatggtgtg accgctggcc tgttcaaacg
     6961 gcataatatt gacatctggt ggtggcggca gaatacgttt tccggtttca atctcccagt
     7021 cgcaggaata aataatcccc atcgcggtgg cgatggggcc ttccatacgc gccatcagat
     7081 caatccattg ccctacgccc gcatcttgtt tgaagtagcg aggatcgacc atattcatgc
     7141 tgccggtgta cgcgatgtaa ttatcgatca tgatcatctt gcgatgttgg cgcaggtcca
     7201 tacggcgtaa aaacacacgc atcagattga cctttaaggc ttcgaccact tcaataccgg
     7261 cattacgcat tagctcgggc cacgggctgc ggaaaaaagc cacactcccg gcggagtcga
     7321 gcatcaatcg gcaatgaata ccacgccgtg cagccgccat taatgattca gccacctggt
     7381 ccgccatgcc gccgggctgc cagatataaa acaccatctc aatattatgg cgcgcgagct
     7441 ggatgtcgcg gattaacgcc tgcatcacat catctgactc ggtcatcagc tgtagctgat
     7501 tccctttgac cccagcgatc ccctgacgac gctcgcaaag cttgaataat ggcgcagcga
     7561 cactgctatt ttcttcggcg aagatatgct tacaggcttt aaggtcgtta agccattttg
     7621 cggtggaagg ccacatcgct ctggcgcgct cagcgcggcg tttgcctaaa tggagctcgc
     7681 caacggcaag ataggcaata attccgacta acggcagaat gtaaataatc aacagccagg
     7741 ccatcgcgga gggaactgcg cgtcgtttca ttagaatgcg taaagttacg cctgcaatga
     7801 gcaaccagta tcccagaatg gccaaccaac tcaccaacgt ataaacggtt gtcatagatt
     7861 agaaatcctt ttgaaagcgc attgttatga gtttacgcat atggattcat ctggcaaata
     7921 aaaacgcggg aaaagcactg gtcagcgtcg ggggcggggg tataatgacc attctgttat
     7981 tgcatagagt agttaacatg aagcggagta gaacggaagt ggggcgctgg cgcatgcagc
     8041 gtcaggctag ccgacgtaaa tcgcgttggc ttgaggggca atcgcgccga aatatgcgta
     8101 tccacagcat caggaagtgc attctaaaca aacagcgtaa ctcgttattg tttgcgatct
     8161 acaatatcta aatgtactgg gcaccattgc ggtgcccaat aagtttactc ttccgtcttt
     8221 aaactctatt tttgcgccga ttctttactg tcagctttcg tttcttgttc atcgttatcg
     8281 ctgaaaatac catgacctga gttcaacaac attgaaacga gcatatcatt attaatctct
     8341 tcaccattta aaactcgcgc cagaatttcg ctgccaaaca gttgcggtga gagaatgata
     8401 tccggatgta ccatcttaat cttattcagg tttttgctat cactgacggc gagaactgtt
     8461 ttgacatcac tgctcatatc ttttgccgag agtacaacaa acgcgttatc tgcatcgtta
     8521 tcactcagcg ccagaatggc ccggcaacga tcgattcccg ctttctttaa tactgaactg
     8581 tcattactgt caccggggat aacatcagcg ttatcgccta agcgttgctc aagttgcttg
     8641 atatcatctt caggcaagtt gctgataacc gttacgtttt gtccgcgttg attcagttgc
     8701 aggatcgtat tgatggcgag aatcgaatgt ccgcaaacaa taaaatgatc tttacgatgc
     8761 attgtatgat tgtttccttt taaaagtttg ttgaatcccc cgcggataag cggaccaaaa
     8821 attgaggtca tagatgtggc aaaaacggta atgccggaaa taatgaccga aatagtgaac
     8881 aatcgtgctg attcagaaac agggacaata tctccatagc cgacggttga catggtttct
     8941 atcgaaaaat agaacgcggt catcaaactt tctattcgcg gattaaaacc ttcgcttaaa
     9001 taaagcgcac cgtaggttga gtaaaacagt aatgtcgtga aactaataaa tgcaaaaatt
     9061 gtcccggctg cggcgctact gtgggagaag tctttgcgca gtatcagcaa aaacaccagc
     9121 gtaaaaatgc aaaatccaat actaaatttc agccagggat aaaaatgcag ggtgtaaatt
     9181 agcgctatca acaacaaaat gatactgatt gcccatgcga gctttgcccg gaacagtaag
     9241 ccaatggagt tcaggacaag aaagacgccg agcataaata gtggggcgtt tgccagcaag
     9301 gagagatcca actctgaaaa tgctttgata tggaaaatat caagcaaatt gaccgacata
     9361 ccatagattg ttttaaaaat aagcaatcca tttaaaaaga cggccagcgc gagaatgtcg
     9421 tgccgtaggg tcacccataa atttgttgct gtttgtttga atgtagccca gtgactcact
     9481 gatacctctg taaccacaat tttgtttaat tatcttagcg tgcacatttt ctgaacatac
     9541 atgcagcgcg tgaatgtgtt aaacaatagt agatgacttt atccgcagta atcaaaggtt
     9601 ttgagtgagc aactctgcgt caggaaaggg cttttttcct gaacatgctc acatcatata
     9661 aagaaataca gataaagtta ttatctgctt gtggtagtga atgcactgac cggctataag
     9721 gaaaggccaa acaagaacac ggttgcaaaa accgtgccct taaatattgg atct
//
LOCUS       DAZPCS010000140         9680 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZPCS010000140 DAZPCS010000000
VERSION     DAZPCS010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9680
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.141"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(44..214)
                     /locus_tag="ACHUQ7_003206"
                     /pseudo
     CDS             complement(44..214)
                     /locus_tag="ACHUQ7_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(433..1410)
                     /gene="epmA"
                     /locus_tag="ACHUQ7_003207"
     CDS             complement(433..1410)
                     /gene="epmA"
                     /locus_tag="ACHUQ7_003207"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFR1136496.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1735..3543
                     /gene="frdA"
                     /locus_tag="ACHUQ7_003208"
     CDS             1735..3543
                     /gene="frdA"
                     /locus_tag="ACHUQ7_003208"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFR1136497.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            3536..4270
                     /gene="frdB"
                     /locus_tag="ACHUQ7_003209"
     CDS             3536..4270
                     /gene="frdB"
                     /locus_tag="ACHUQ7_003209"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFR1136498.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4281..4676
                     /gene="frdC"
                     /locus_tag="ACHUQ7_003210"
     CDS             4281..4676
                     /gene="frdC"
                     /locus_tag="ACHUQ7_003210"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFR1136499.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FALFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4687..5046
                     /gene="frdD"
                     /locus_tag="ACHUQ7_003211"
     CDS             4687..5046
                     /gene="frdD"
                     /locus_tag="ACHUQ7_003211"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFR1136500.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            5109..6241
                     /locus_tag="ACHUQ7_003212"
                     /pseudo
     CDS             5109..6241
                     /locus_tag="ACHUQ7_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
     gene            6330..6863
                     /gene="blc"
                     /locus_tag="ACHUQ7_003213"
     CDS             6330..6863
                     /gene="blc"
                     /locus_tag="ACHUQ7_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFR1136501.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6860..7177)
                     /gene="sugE"
                     /locus_tag="ACHUQ7_003214"
     CDS             complement(6860..7177)
                     /gene="sugE"
                     /locus_tag="ACHUQ7_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFR1136502.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7353..7499)
                     /gene="ecnB"
                     /locus_tag="ACHUQ7_003215"
     CDS             complement(7353..7499)
                     /gene="ecnB"
                     /locus_tag="ACHUQ7_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFR1136503.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7610..7735)
                     /gene="ecnA"
                     /locus_tag="ACHUQ7_003216"
     CDS             complement(7610..7735)
                     /gene="ecnA"
                     /locus_tag="ACHUQ7_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFR1136504.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(7787..8353)
                     /gene="efp"
                     /locus_tag="ACHUQ7_003217"
     CDS             complement(7787..8353)
                     /gene="efp"
                     /locus_tag="ACHUQ7_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFR1136505.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8395..9423
                     /gene="epmB"
                     /locus_tag="ACHUQ7_003218"
     CDS             8395..9423
                     /gene="epmB"
                     /locus_tag="ACHUQ7_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFR1136506.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2465 a         2479 c         2466 g         2270 t
ORIGIN      
        1 ggcattatct gaacataaaa cactatcagt aagttggagt cattaccatt aaggcgaatg
       61 gaataaagaa taacaatgca gaaaatatat aaaagggaat cgcactataa cccattaagt
      121 aataagccga tgggctattg gcaaatccaa atacggaagt aaagatcatc aatatgagtc
      181 ctatcagact catctttttt atcgtgtgag gcatgaaacc atccttcatt tcagcgcgtg
      241 tataacacgt gcccactgaa ctgcactgcg caacattgag caggcagcgt aaaaaccaat
      301 acagaattga tacttggatg atatcaaata tccagtctta aattgtggcc tttaacggaa
      361 aaatgaaaac atatgctgca gaatggcgct tatcacgcca ttcttcgctg ttaattcagt
      421 aatttttcag aattatgccc ggtcaacgct aaaggcgatg acttcagcca gtgtctccgc
      481 gcccagcgcc aacatcacca gacgatcaac acctaatgcc acgccggaac agtcaggcat
      541 accgactttc aaggcttcaa tcagattctg gtcaatgggg tgctgcggca aaccgcgcgc
      601 cgcgcgctta cggttatctt gttcaaagcg ttgttgctgc tcacgggcat ccgttaattc
      661 atggaaacca ttcgccagct caataccttt ataataaacc tcaaagcgtt cagcgacccg
      721 atgatcttcg gtactgattt gcgccagtga tgcctggctg gctggaaagt ggtacacaaa
      781 ggtcggtttt tctttaccaa tatttggctc tacgccaaag gtaaacagca attgtagcaa
      841 cgtgtcgcgg tcttcttcgg tatcagcaac attgctcaaa tccagtttcg ctgcgacttc
      901 ccgcagttgc gttttgtcgg cagagagcgg gtcaatttcc agataacgca agaaagcttg
      961 ttgataagaa aggctttctg ctgccgggca gtccagcacc tgttgtaaga gatcgtccac
     1021 ctcgttcatc aaccggtaca tatcatagtg cggtcgatac cactccagca tagtgaactc
     1081 agggttgtga taacgcccca tctcttcatt acggaagctg cggcacagct ggaataccgg
     1141 cccacaacca gcaaccagca ggcgtttcat atggtattcc gggctggtca ttaaccagag
     1201 attcatcccc tgcgaatgcc cggggccaac gaaacgtgtc tcaaacggga ccaaatgaat
     1261 atcggttacc gtcgcctggc tcatacaagg cgtctccacc tccagcactc cacgatcggc
     1321 aaagaaacga cggatctccg ccataatcgc cgcgcgtttt aataagttag gaatggatgc
     1381 gctcggctgc caggatgccg tttcgctcat agttaaatct ccagtttttg acaagggcac
     1441 gaagtctact cgcaacgcga cggcgagaca aattttacgc aggaatcaaa cagcggtggg
     1501 cagtgactaa aaaaagcacg atctgatggt ttagtaatta aattaatcat cttcagtgat
     1561 aatttagccc tcttgcgcac taaaaaaatc gatctcgtca aatttcagac ttatccatca
     1621 gactatactg ttgtacctat aaaggagcag tggaatagcg ttcgcagacc gtaactttca
     1681 ggcacttacc ctgaagtacg gggctgtggg ataaaaacaa tctggaggaa tgtcgtgcaa
     1741 acctttcaag ccgatcttgc cattgtaggc gccggtggcg cgggattacg tgctgcaatt
     1801 gctgccgcgc aggcaaatcc aaatgcaaaa atcgcactaa tctcaaaagt atacccgatg
     1861 cgtagccata ccgttgctgc agaagggggc tccgccgctg tcgcgcagga tcatgacagc
     1921 ttcgaatatc actttcacga tacagtagcg ggtggcgact ggttgtgtga gcaggatgtc
     1981 gtggattatt tcgtccacca ctgcccaacc gaaatgaccc aactggaact gtggggatgc
     2041 ccatggagcc gtcgcccgga tggtagcgtc aacgtacgtc gcttcggcgg catgaaaatc
     2101 gagcgcacct ggttcgccgc cgataagacc ggcttccata tgctgcacac gctgttccag
     2161 acctctctgc aattcccgca gatccagcgt tttgacgaac atttcgtgct ggatattctg
     2221 gttgatgatg gtcatgttcg cggcctggta gcaataaaca tgatggaagg cacgttggtg
     2281 cagatccgtg ctaacgcggt cgttatggct accggcggtg cgggtcgcgt ttatcgttac
     2341 aacaccaacg gcggcatcgt taccggtgac ggtatgggta tggcgctaag ccacggcgtt
     2401 ccgctgcgtg acatggaatt cgttcagtat cacccaaccg gtctgccagg ttccggtatc
     2461 ctgatgaccg aaggctgccg tggtgaaggc ggtattctgg tcaacaaaaa tggctaccgt
     2521 tatctgcaag attacggcat gggcccggaa actccgctgg gcgagccgaa aaacaaatat
     2581 atggaactgg gtccacgcga caaagtttct caggccttct ggcacgaatg gcgtaaaggc
     2641 aacaccatct ccacgccgcg tggtgatgtg gtttatctcg acctgcgtca cctcggcgag
     2701 aaaaaactgc atgaacgtct gccgttcatc tgcgaactgg cgaaagcgta cgttggcgtc
     2761 gatccggtta aagaaccgat tccggtacgt ccgactgcac actacaccat gggcggtatc
     2821 gaaaccgatc agaactgtga aacccgcatt aaaggtctgt tcgccgtggg tgaatgttcc
     2881 tctgttggtc tgcacggtgc aaaccgtctg ggctccaact ccctggcgga actggtggtc
     2941 ttcggccgtc tggccggtga acaagcgaca gagcgtgcag caactgccgg taatggcaac
     3001 gaagcggcaa ttgaagcgca ggcagctggc gttgaacaac gtctgaaaga tctagttaac
     3061 caggatggcg gcgaaaactg ggcgaagatc cgcgacgaaa tgggcctggc aatggaagaa
     3121 ggttgcggta tctaccgtac gccggaactg atgcagaaaa ccatcgacaa gctggcagag
     3181 ctgcaggaac gcttcaagcg cgtgcgcatc accgacacct ccagcgtgtt caacaccgac
     3241 ctgctctaca ccattgaact gagccacggt ctgaacgttg ctgaatgtat ggcgcactcc
     3301 gcaatggcac gtaaagagtc ccgcggcgca caccagcgtc tggacgaagg ttgcaccgag
     3361 cgtgacgacg tcaactttct caaacacacc ctcgccttcc gcgatgctga tggcacgact
     3421 cgcctggagt acagcgacgt gaagattact acgctgccgc cagctaaacg cgtttacggt
     3481 ggcgaagcgg atgcagccga taaggcggaa gcagccaata ataaggagaa ggcgaatggc
     3541 tgagatgaaa aacctgaaaa ttgaggtggt gcgctataac ccggaagtcg ataccgcacc
     3601 gcatagcgca ttctatgaag tgccttatga cgcaactacc tcattactgg atgcgctggg
     3661 ctacatcaaa gacaacctgg caccggacct gagttaccgc tggtcctgcc gtatggcgat
     3721 ttgtggttcc tgcggcatga tggttaacaa cgtgccaaaa ctggcatgta aaaccttcct
     3781 gcgtgattac accgacggta tgaaggttga agcgttagct aacttcccga ttgaacgcga
     3841 tctggtggtc gatatgaccc acttcatcga aagtctggaa gcgatcaaac cgtacatcat
     3901 cggcaactcc cgcaccgcgg atcagggtac taacatccag accccggcgc agatggcaaa
     3961 gtatcaccag ttctccggtt gcatcaactg tggtctgtgc tacgccgcgt gcccgcagtt
     4021 tggcctgaac ccagagttca tcggtccggc tgccattacg ctggcgcatc gttataacga
     4081 agatagccgc gaccacggta agaaggagcg tatggcgcag ttgaacagcc agaacggtgt
     4141 atggagctgt actttcgtgg gctattgctc cgaagtctgc ccgaaacacg tcgatccggc
     4201 tgcggccatt cagcagggca aagtagaaag ttcgaaagac tttcttatcg cgaccctgaa
     4261 accacgctaa ggagtgcaac atgacgacta aacgtaaacc gtatgtacgg ccaatgacgt
     4321 ccacctggtg gaaaaaattg ccgttttatc gcttttacat gctgcgcgaa ggcacggcgg
     4381 ttccggctgt gtggttcagc attgaactga ttttcgcgct gtttgccctg aaaaatggcc
     4441 cggaagcctg ggcgggattc gtcgactttt tacaaaaccc ggttatcgtg atcattaacc
     4501 tgatcactct gacggcagct ctgctgcaca ccaaaacctg gtttgaactg gcaccgaaag
     4561 cggccaatat cattgtaaaa gacgaaaaaa tgggaccaga gccaattatc aaaagtctct
     4621 gggcggtaac tgtggttgcc accatcgtaa tcctgtttgt tgccctgtac tggtaaggag
     4681 cctgagatga ttaatccaaa tccaaagcgt tctgacgaac cggtattctg gggcctcttc
     4741 ggggccggtg gtatgtggag cgcaattatt gcgccggtga tgatcctgct ggtgggtatt
     4801 ctgctgccac tggggctgtt tccgggtgat gcgctgagct acgagcgcgt tctggcgttc
     4861 gcgcagagct tcattggtcg cgtattcctg ttcctgatga tcgttctgcc gctgtggtgt
     4921 ggtttacacc gtatgcacca cgcgatgcac gatctgaaaa tccacgtgcc tgcgggcaaa
     4981 tgggttttct acggtctggc tgctatcctg acagttgtca cgctgattgg tgtcgttaca
     5041 atctaacgca tcgccaatgt aaatccggcc cgcctatggc gggccgtttt gtatggaaac
     5101 cagaccttat gttcaaaacg acgctctgcg ccttattaat taccgcctct tactccacat
     5161 ttgctgcccc tcaacaaatc aacgatattg tgcatcgcac aattaccccg cttatagagc
     5221 aacaaaagat ccccggtatg gcggtggcgg taatttatca gggtaaacct tattacttta
     5281 cctggggcta tgcggacatc gccaaaaagc agcccgtcac acagcaaacg ttgtttgagt
     5341 taggttcggt cagcaaaaca tttacgggcg tgcttggtgg cgacgctatt gctcgagggg
     5401 aaatcaagtt aagcgatccc gcaacaaaat actggcctga acttaccgct aaacagtgga
     5461 atgggatcac actattacat cttgcgacct acaccgctgg cggcctgcca ttgcaggtgc
     5521 cggatgaagt gaaatcctca agcgacttgc tgcgcttcta tcaaaactgg cagcctgcat
     5581 gggctccagg aacacaacgt ctgtatgcca actccagtat cggtttgttc ggcgcactgg
     5641 ctgtgaagcc gtctggtttg agttttgagc aggcgatgca aactcgtgtc ttccagccac
     5701 tcaaactcaa ccatacgtgg attaatgtac ctcccccaga agaaaagaat tacgcctggg
     5761 gatatcgcga aggtaaggca gtgcatgttt cgccaggggc gttagatgct gaagcttatg
     5821 gtgtgaagtc gaccattgaa gatatggccc gctgggtgca aagcaattta ccccccttga
     5881 tatcaatgag aaaacgcttc aacaagggat acaactggca caatctcgct actggcaaac
     5941 cggcgatatg tatcagggcc tgggctggga aatgctggac tggccggtca atcctgacag
     6001 catcattaac ggcagtgaca ataaaattgc actggcagca cgcccggtaa aagcgattaa
     6061 gcccccaact cctgcagtac gcgcatcatg ggtacataaa acgggggcga ccggcggatt
     6121 tggtagctat gtcgcgttta ttccagaaaa agagctgggt atcgtgatgc tggctaacaa
     6181 aaactatccc aatccagcga gagtcgccgc cgcctgtcag attctcaacg ctctacagta
     6241 aaatttcatc gggtccgaat tttcggacct tttctgcgct tttccttgct gtcatctaca
     6301 cttagaaaaa aaccagtaag gaaacattta tgcgcctgct ccctctcgtt gccgcagcga
     6361 cagctgcatt tctggtcgtt gcctgtagtt ttcctacgcc gccgcgtggc gtgaccgtag
     6421 tgaataattt cgacgccaaa cgttatctcg gtacctggta tgagattgcc cgttttgatc
     6481 accgttttga acgtggactg gaaaaagtca ccgcaacata cagcctgcgt gatgacggcg
     6541 gcctgaatgt cattaataaa ggctataacc ctgacagagg aatgtggcag cagagtgaag
     6601 ggaaagcgta ctttaccggc gcaccaactc gcgctgcgct gaaagtgtca ttctttggtc
     6661 ctttctatgg cggttataac gttattgcac tcgatcggga ataccgccat gcgctggttt
     6721 gcggcccgga ccgcgactac ctgtggatac tctcccgcac gccaaccatt tctgacgaag
     6781 tgaaacagga gatgctggca gtcgcgaccc gggaagggtt tgatgtcagt aagtttattt
     6841 gggtacagca gcccggtagt tagtgagtgc tgagtttcag accaataatc cccaatacga
     6901 ttaacgccag actcgccaga cgcatcgggt tagcggactc accgagcagc acaatgccgg
     6961 tgatggccgc gccgactgcg ccaatacccg tccacacggc ataagccgtc cctaccggta
     7021 acgatttcat cgcccaggca agtagcgcca tactgactat catcgccgtc acggtaataa
     7081 cactcggcgt caaacggcta aagccgtggg tatatttcag gccaacggcc cataccactt
     7141 ccagcagacc agcaataact aagataatcc aggacatatc aggctccgga aatttggggc
     7201 cgtccccggt gaaaagaggc gtttgcaggt cgtcctgcaa agctgatggt tgaacggata
     7261 ttttgccagg tggcagggga atttcaacta ttacctttat gacgatcgca gaaaaaacaa
     7321 acggcacgac actgctgtcg taccgttatt gcttattgct gcgctttcgt tgctgcgcca
     7381 gaaatcgcgt taccgccatc agaaatgtct tcaccaacgc cacgcgtggt gttgcaggca
     7441 gttaatactg ttgaaagcac cagaacagaa aagatcgctg caattgtctt cttcaccata
     7501 acgtcttcct tttaggtaat gttatttatg tttgcctata gcaaattaat aatagacaac
     7561 atcgccaaaa atgatggaaa tctgatgatt tttcggaaga gaagaaaaat tagctggcag
     7621 cgcgagagat ggagttaccg agatgtttga tgtcttcgcc gaaaccgcaa gcggtgttac
     7681 agcccgtgag cagcgtgctg gcaagcaaaa caagaacgat aaggcgtttc atcatctctg
     7741 acctgaataa gtgatggtgc agcctgcagg ccgcaccaca accgcattac ttcacgcgag
     7801 agacgtattc accagagcgg gtatccactt tgatgacttc gccgatttgt acgaacagcg
     7861 gaactttaac cacagcgcca gtagacaggg tagccggttt gccgccagta cctgcggtat
     7921 cacctttcag gcccggatcg gtatcaacga tttccagttc aacgaagttc ggcggagtaa
     7981 cggagatcgg ctgaccattc cacagagtta cgatacactc tgcctgatcc agcagccatt
     8041 tagcattgtc gccaattgct tttgcatcag cagacagctg ctcgaaagtt tcgttgttca
     8101 tgaagtgcca gaactcaccg tcgttgtaca ggtaagtcag gttcatatcg acaacatcag
     8161 cgccttcagc ggaatcagta gatttgaagg ttttttctac gcgagtaccg gtcagcagac
     8221 gacgcagttt aacgcgagca aatgcctggc ctttacccgg ttttacgaat tcactcgctt
     8281 caaccgcgta aggttcgccg tctaacatga ttttaagacc agcacgaaaa tcgttgctat
     8341 agtacgttgc cataaggccc tctgaaattt gttaattggt agctaagcca caaaatggcg
     8401 catattgtaa ccctaaatac cccatccaga gaagattggt taacgcaact tgccgatgtt
     8461 gtgaccgatc ctgatgaact tctgcgtctt ttgaatatag acgcggacga aaaactgttg
     8521 gccggacgca gcgccaaaaa gctgtttgcc ctgcgtgtgc cccgctcatt tatcgatcgc
     8581 atggagaaag gcaatccgga cgatcctctt ttgcgtcagg tacttacctc gcaagatgag
     8641 tttgtcgtcg cgtccggatt ctccaccgac ccactggaag aacagcacag cgtagtgcct
     8701 ggtttgttgc ataaatacca caaccgggcg cttttgctgg tcaaaggcgg ctgcgcggta
     8761 aattgccgct attgcttccg tcgccacttc ccctatgccg aaaatcaggg caacaagcgt
     8821 aactggcaga ctgcacttga gtatgttgct gcgcatccgg aactggacga gatgattttc
     8881 tccggcggcg atccgctgat ggcgaaagag cacgagctgg actggttgct cacacaactg
     8941 gaagccatcc cgcatatcaa acgtctgcgg attcacagcc gtctgccgat tgtgatcccg
     9001 gcacgtatca ccgaggcgct ggttgaacgc tttgcccgtt ctacgctgca aatcttgctg
     9061 gtgaatcaca tcaatcatgc caatgaggta gatgaaacgt tccgtcaggc gatggctaaa
     9121 ttgcgccgtg tcggtgtcac cctgcttaac cagagcgttc tgttacgtgg tgtgaacgat
     9181 aacgcacaaa cgttggcaaa cctgagtaat gcgttgttcg atgccggcgt gatgccgtat
     9241 tacctgcatg tgctagataa agtacagggc gcggcgcatt ttatggtgag tgatgacgaa
     9301 gcacggcaga ttatgcgtga gttgctgaca ttggtgtcgg gatatctggt gccgaaactg
     9361 gcgcgagaaa ttggcggcga acccagcaaa acgccgctgg atctccagct acgccagcag
     9421 taactgatta aacagggaag ttaaacatta aaattgcctg atgcgctacg cttatcaggc
     9481 ctacatcgtg cattgaaatt gaatggattt gcaatgaact gtaggccgga taaggcgttt
     9541 acgccgcatc cggcaagagc aacaaacgca agtacagcaa ccactgagaa atttaatctt
     9601 tgcacgttgc ataaccttta attattcata tgcattgctg ttgttacttt ttatatagct
     9661 aacaccaata attaaccttt
//
LOCUS       DAZPCS010000141         9551 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZPCS010000141 DAZPCS010000000
VERSION     DAZPCS010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9551
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.142"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(68..970)
                     /locus_tag="ACHUQ7_003219"
     CDS             complement(68..970)
                     /locus_tag="ACHUQ7_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HFR1136507.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTTTSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEMTITHPQQALSVSLCSDQPWVQVYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            complement(1108..2349)
                     /gene="yihS"
                     /locus_tag="ACHUQ7_003220"
     CDS             complement(1108..2349)
                     /gene="yihS"
                     /locus_tag="ACHUQ7_003220"
                     /EC_number="5.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HFR1136508.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTVYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVITKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            complement(2366..3244)
                     /gene="yihT"
                     /locus_tag="ACHUQ7_003221"
     CDS             complement(2366..3244)
                     /gene="yihT"
                     /locus_tag="ACHUQ7_003221"
                     /EC_number="4.1.2.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HFR1136509.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSSVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            complement(3268..4164)
                     /gene="yihU"
                     /locus_tag="ACHUQ7_003222"
     CDS             complement(3268..4164)
                     /gene="yihU"
                     /locus_tag="ACHUQ7_003222"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFR1136510.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            4332..5228
                     /gene="yihV"
                     /locus_tag="ACHUQ7_003223"
     CDS             4332..5228
                     /gene="yihV"
                     /locus_tag="ACHUQ7_003223"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HFR1136511.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            5262..6047
                     /locus_tag="ACHUQ7_003224"
     CDS             5262..6047
                     /locus_tag="ACHUQ7_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFR1136512.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEKKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            6146..6745
                     /gene="yihX"
                     /locus_tag="ACHUQ7_003225"
     CDS             6146..6745
                     /gene="yihX"
                     /locus_tag="ACHUQ7_003225"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFR1136513.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            6739..7611
                     /locus_tag="ACHUQ7_003226"
     CDS             6739..7611
                     /locus_tag="ACHUQ7_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFR1136514.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            7608..8045
                     /gene="dtd"
                     /locus_tag="ACHUQ7_003227"
     CDS             7608..8045
                     /gene="dtd"
                     /locus_tag="ACHUQ7_003227"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFR1136515.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            8090..9031
                     /gene="fabY"
                     /locus_tag="ACHUQ7_003228"
                     /gene_synonym="yiiD"
     CDS             8090..9031
                     /gene="fabY"
                     /locus_tag="ACHUQ7_003228"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFR1136516.1"
                     /translation="MYHLQVPQTEEELERYYHFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMVVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            9447..>9551
                     /locus_tag="ACHUQ7_003229"
     CDS             9447..>9551
                     /locus_tag="ACHUQ7_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1136517.1"
                     /translation="MVANINLIKQESYSVVNLEKQLMSNKSPKQTLLNQ"
BASE COUNT         2261 a         2379 c         2525 g         2386 t
ORIGIN      
        1 agatacataa tgttgccttt agcgagtgaa atgttcttca atgattttga ctgctaatca
       61 atacaagtta ttttctctgt ccgtaaatat taaaaaacag gcggtgcggt tttcctggtt
      121 ccagtaataa taagtcgata cctgaattaa aggcgttagg cgggcaactc ataggttcaa
      181 ctgccagtcc ctgacgctgt aatttttcac cgctataaac ctgcacccag ggttgatcgg
      241 aacaaaggct taccgaaagc gcctgctgtg gatgggtaat ggtcatttcc cacaaatcat
      301 tagcggtttt aaaggtgtgg tcgatttttg tcgcggctat ttttttcgcc tgcgtgaaat
      361 ttaaatccag ctcatcaacg tggtgaagcg ttgtcggatt tacgtgttca tcgacggcat
      421 aaacctggtt tgccggaagc tggaacagat attcatcaac agatgttaag ttgcaggtta
      481 aataaggatg aatacccacg ccataaggcg ctgcgacagt gccgatattc tggcttgcga
      541 tctcaacgga aagacccgtg tgtgcgttga gcgaataaac aacttgtgaa gccagcataa
      601 acggatagcc gtaactgggc ggcagaaaag ctgtcagggt aacgctggtg gtggttaatt
      661 cactaatttg ccagtctcgc caggcaagca gcccgtgaat agcggctttt gaactatgtt
      721 cattaatggg taactgatat tcctgcccct gataacggta acagccgttg gcgatccggt
      781 tcggccacgg aataagcact ttacccagat gcgccagcgg catctcttcg ggtttgtgcg
      841 ggatgactaa atgacaaccc tgaaaggtta attcagccag accagcaccg acggtcacaa
      901 tggtcgcgtg ataatccccc gcggcaagtg aaacggtctg tccactgcaa tgcatattgg
      961 tgatttgcat acaaggcacc ttaattaacg acatacagcc tcccgtgagg ctgatgtcaa
     1021 acccaaaaac tgcaccggac ggtcccctcg cccctttggg gagagggtta gggtgagggg
     1081 aaaacccgca ccctattttc agatcagtta tttcgcatta atatccagca gacccgctgc
     1141 aaccgctgga gccatgccgg gcgctaacgg gatacgcggg atcaccaggc aatgcagcag
     1201 gtgataaata tcctgtttgc cgtcccagac tttggtgatg accttattgt ccgcatccag
     1261 ctcctgccac caggaaccat tttcatagtc catcaggtac ttaatgcagt actcccacca
     1321 tgtttgatac caggtttcat actggcgatc gccagtgacg gtgtagagcg cgtagaccgt
     1381 acccattgct tcgacgatag gccaacgtac acgttcgcgg accaccggtt ttccttccca
     1441 gtcaacggta taaacaatcc cgtccgctcc atcgggtgcc caggcatcgc gcacggtggc
     1501 gttaaacaga cctttggcat cttctagcag ccatgctggt ggttgttcgc aacgggcttc
     1561 cagggcagca tggatgtgca gcattaaacg gccccattcg atccagtggc ctggtgtacc
     1621 tccgaacgcg cggaagcgat gcgccgggtt atctttgttg taatccggca gcggattcca
     1681 ctgggtatcg aagtgttcgt taacgcgata atgattattt ctggcgacgt cgtggataat
     1741 cacggaagcc acgcgaatcg cgcgatccag ccattttttg tcgtgagtga cgtcataaac
     1801 aatcaagaaa gcttccaccg cgtgcatatt ggcattgccg ccgcggtact cttcggtttt
     1861 gctgaaggct tcgtcccagg actccaggca catctgctct tcttcgctcc agaaatattt
     1921 ctcgataatc tcaatggtgt aatcgagcag cttgcgcgct tccgggtgac ccgttgtgac
     1981 ggcgctggcg gcacccagca gagcaaagaa gtgttgatag ccctgtttgg aggcatccac
     2041 cacgccctcg tcattcacgc aggcatacca gccgccatat tttttatcac gcagtgcgcc
     2101 gttcatggct ttaataccgt gatcaaccaa cgcgtaagcg ccaggtcgac ccatcgccgc
     2161 agcaacggaa taaacgtgca acatacgagc tgtgatccac agatgggtgc ccatctcttc
     2221 tttgatttgc cctttattgc ctaaccagcc aaaaccagtc ggcactacgg aatttttacc
     2281 aaaatcaaag atgcggtcgg tttcctgttc cagccaacgg ttgtggctta gggtgttaaa
     2341 ccatttcatt ccttttatcc tcatcttagc ggcgtttagc catcatttcg tcgacgatat
     2401 cgccaagttg ttgtaatttc ggtgcgcaaa cgtcacggag catcagctcg ttgtctggta
     2461 aaccgacgac cgatgcccag actgcacggc ctgccaggaa tcccgatgcg cccgccgtca
     2521 ttgccacgcg tacggcacgc gggaacagtt tttcgtcgac actggaagag aggatcaccc
     2581 atggcatatt gatatggtca ttcagacgtt gtgaagcaca gagaagctct tgttgcggac
     2641 ctttgccata aagcggcatt tcaactttgt agaggtcagc gccactgtcg cccagctctt
     2701 tggcggcatc gatgatcgct tgttcgcgat cgaatttatc gccacgacgc ggtgggcgga
     2761 cgactggctc aatgatgctt accagaccgt gtgagtggca cagttcgttg aactctttca
     2821 ccatatccag acgttgctgc gcatcttcat cactacgcca cagcaccagc agttttaagg
     2881 ctttgccacc gtcttgtttg atctgtagcg gattgatttt gcggtcaata accacgctat
     2941 cgaccggaat accgttgcca ggaatgaact catcggcggc gacaatcatg gcgcaacttt
     3001 tggcaatcgc gttttgctca accacctggc ggtagcagaa ttgttgatct accagaatcg
     3061 ccgaggcata aggcgagagg gcctttgcag cgttaacttt gaaatcagtt aaaacgctat
     3121 cggctaccgg agcgggtgcc ccagccgcgg caaacatcat gcgcatggct tcgcgctgat
     3181 cgaccgccag catggcaaaa ccgcccgatg cgcgcgtaat gtcgttgatg gtgtacttat
     3241 tcattcattt attcctttat ccagtcgtta catttttact ttggcagtca tcccggcact
     3301 gacacggacc tgttccagaa tggcggacca gtcctggcga ccgcgacccg ctgcgcgcgc
     3361 ctggctataa acctcccgtg aggcggcccc cagcggcatt ggcacatgca gctggttggc
     3421 gacatcaagg gcgatgccaa gatccttatg ggcaagatcg atcatgaagg cgggagaaag
     3481 atctccgctg aggactttgt ttggccagga agtggtgaag tgacctttac cggcggcggt
     3541 accgctcatc actttgacgg caacatcgaa gggaagattc agggcttcgc acaaaacggc
     3601 ggcttctgcc gaaagcgcat tgagcgcgat gctcatgtag ttgttgatga gcttaacgcg
     3661 gatccccatg cccggaccgc cagcgttgat caactcactg cccatcgcca tcaggatcgg
     3721 tgtggcacgt tcaacttgtt cagcggtgcc gccagccagc agtaacagag taccggtaat
     3781 ggcatttgca gaagtacggc ctaccggaac atccatcatg ctgaagcctt tggcttgcat
     3841 atcggcaatc aatttatcgg tttgcagcgg atggatggtg gacatatcaa tgaccagcgc
     3901 atcggtagat aagccttcgc aaacgccgtt ttcaccgaac aacacgttgc gcaccagatc
     3961 gccattcggc aacatggtaa tgataaattc ggcatcttta gcggcctgcg ccgggttggc
     4021 ggcgggagtc gcacctttgt ctaccagatg ccgcacagct tcggcgttca catcaaagac
     4081 gcgaagttga tgcccttgct gcaataaatt gctcgccatt ggcgaaccca tttgtcctaa
     4141 accgataaac gcgattgctg ccataaccct ctcctgaata cagtgatgtc actttttgtc
     4201 atttatgaca tgctttgctt gtctgttttt gatcgaattt gtaatttatc gtcaaaaaat
     4261 tgacagccgt cactttttaa acaattggtg aaattaaaat gaacgcatcc caaaatgttt
     4321 aaggaatgac catgattcgt gttgcttgtg taggtataac cgtgatggat cgcatctatt
     4381 acgtggaagg gttaccgacg gagagcggta aatacgtggc gagaaattat acggaagttg
     4441 gtggcgggcc agcggcgacg gcagcggttg cggcggcaag gctgggggcg caggtcgatt
     4501 ttattggtcg cgtaggtgat gacgacgcgg gcaacagcct gctggcagag ctggaatcct
     4561 ggggcgttaa cacccgttac accaaacggt ataaccaggc gaaatcttcg caatccgcca
     4621 tcatggtgga taccaaaggc gagcggataa tcattaacta ccccagcccg gatctgctgc
     4681 ctgacgcaga gtggttggag gaaattgatt tctctcagtg ggatgttgtg ctggcagatg
     4741 tacgctggca cgacggcgct aaaaaagcct tcaccctggc ccgtcaggcg ggtgtgatga
     4801 ccgttctgga cggggacatt acgccgcagg atatcagtga gctggtggca ttaagcgatc
     4861 acgcggcctt ttcagaaccg ggtctggcgc gcttaacggg cgtgaaagag atggccagtg
     4921 cgctaaaaca ggcacaaacg ctcacaaatg gtcatgtcta tgtgacccag ggtagcgcag
     4981 gctgcgactg gctggaaaat ggtgggcgtc agcatcaacc ggccttcaaa gttgatgtgg
     5041 tagataccac aggtgcggga gatgtttttc acggcgcttt ggcggtggcg ctggcaacaa
     5101 gtggggattt agcggagtca gtccgcttcg ccagcggtgt agcggcgtta aaatgcacac
     5161 gtcccggtgg acgagccggg atccctgact gtgatcaaac ccgatctttt ttgtcacttt
     5221 ttgtataaaa tgccagggtg atggtttttc gaggaatttt catgagcctt accgaactaa
     5281 ccggtaaccc gcggcacgac caactcctca tgctgatcgc cgagcgtggg tatatgaata
     5341 ttgatgagct ggcaaatctg ctggatgtct ccacgcagac ggtccgccgg gatattcgta
     5401 aattaagcga gcaaggcctg attacgcgcc atcacggtgg cgcgggtcgg gcttccagcg
     5461 tcgttaatac ggcgttcgag cagcgtgagg tttcgcaaac cgagaaaaaa aaagcgattg
     5521 ccgaagcggt ggcagactat attcctgatg gttcaacaat atttatcacc attggtacga
     5581 ctgttgagca tgttgcccgg gcgttactta accataatca tttgcggata atcaccaaca
     5641 gcctgcgtgt ggcgcatatt ctttaccaca acccgcgctt tgaagtgatg gtgcccggcg
     5701 gtacgttgcg ctctcataat agcgggatca ttggcccttc agcggcgtcc tttgtggctg
     5761 attttcgtgc tgattatctg gtaacaagcg ttggggcgat tgagagcgat ggcgcgttga
     5821 tggagtttga tgtaaacgaa gctaacgtgg tgaaaacgat gatggcgcac gcgagaaata
     5881 ttctgctggt cgccgatcac actaagtatc atgcttcggc ggcggttgaa attggtaacg
     5941 tggcacaggt cactgcgctc tttaccgacg agctgccgcc cgctgcgcta aaatcacgct
     6001 tacaagacag ccaaattgaa atcattcttc cccaggaaga cgcgtagatt tttgtgaccg
     6061 ttaacctggc ttcatacctt gccacatagc caaacccatc ctttcccgct acagttaatt
     6121 tcttgtggcg cgaaaggagg caaaaatgct ctatatcttt gatttaggta atgtgattgt
     6181 cgatatcgac tttaaccgtg tgctgggagc ctggagcgat ttaacgcgta ttccgctggc
     6241 atcgcttaag aagagttttc atatggggga ggcgtttcat cagcatgagc gtggggaaat
     6301 tagcgacgaa gcgttcgcag aggcgctgtg tcatgagatg gctctaccgc taagctacga
     6361 gcagttctct cacggctggc aggcggtgtt tgttgcgctg cgcccggaag tgatcgccat
     6421 catgcataaa ctgcgtgagc aggggcatcg cgtggtggtg ctttccaata ccaaccgcct
     6481 gcataccacc ttctggccgg aagaataccc ggaaattcgt gatgctgctg accatatcta
     6541 tctgtcgcaa gatctgggga tgcgcaaacc tgaagcacga atttaccagc atgttttgca
     6601 ggcggaaggt ttttcaccca gcgatacggt ctttttcgac gataacgccg ataatataga
     6661 aggagctaat cagctaggca ttaccagtat tctggtgaaa gataaaacca ccatcccgga
     6721 ctatttcgcg aaggtgttat gctaaaaacc attcaggaca aagccaggca ccgtacccgt
     6781 ccactatggg cctggctaaa actactctgg caacgtattg atgaggacaa catgacaacc
     6841 ctggcaggta accttgccta tgtgtcgttg ctctcattag tgccgctggt tgccgttgtt
     6901 tttgcgcttt tcgccgcttt tcccatgttt tccgacgtca gcattcagtt acgtcacttt
     6961 atttttgcca actttctgcc tgctactggc gatgttattc agcggtatat cgaacaattt
     7021 gttgctaatt ccaacaagat gaccgccgtt ggggcgtgcg ggctgatcgt caccgcgtta
     7081 ttgttgatgt actccatcga tagcgcgttg aataccatct ggcgcagtaa acgagcgcga
     7141 ccaaaaattt actcgttcgc cgtgtactgg atgattttaa cgctggggcc gctgctggca
     7201 ggggccagtc tggcgatcag ttcctatttg ctctccctgc gctgggcgag cgatctcaat
     7261 actgtcatcg acaacgtgct gcgtattttt ccgttgctgt tgtcgtggat ctccttctgg
     7321 ttgctgtaca gcattgttcc taccatccgc gtacctaacc gcgacgcgat tgtcggcgcg
     7381 tttgtcgccg cactcctgtt cgaagcagga aagaaaggtt tcgcgcttta tatcaccatg
     7441 ttcccgtcat atcagctcat ttacggtgtg ctggcggtga tccccattct ctttgtttgg
     7501 gtctactgga catggtgtat cgtcttgctt ggcgcggaaa ttactgtcac tctcggggaa
     7561 taccgcaaac taaaacaagc agctgaacaa gaagaagacg acgaaccatg attgcattaa
     7621 ttcaacgcgt aacccgtgcc agcgtcaccg tggagggaga agtgacgggc gaaattggcg
     7681 cgggactttt ggtgttattg ggtgtcgaaa aggatgacga cgaacagaaa gcaaaccgtc
     7741 tgtgcgagcg tgtgctcggc taccgcatct ttagcgatgc cgaaggcaag atgaatctca
     7801 acgtgcaaca ggcgggcggc agtgtactgg tggtttccca gtttaccctc gccgcagata
     7861 ccgaacgggg gatgcgccca agtttctcca aaggcgcatc accggatcgc gcagaggcgt
     7921 tatatgacta tttcgtcgaa cgctgccgcc agcaagagat gaacacgcaa acaggacgct
     7981 tcgctgccga tatgcaggta tcgctggtca atgatggccc cgtgacattc tggttgcagg
     8041 tatgagccag cttccagggt tgtcacggga aacaagagag agtatcgcta tgtatcacct
     8101 tcaggttcca caaacagaag aagagttaga gcgttactat cactttcgct gggaaatgtt
     8161 gcgtaagccc ctgcatcaac caaaaggttc ggaacgcgac gcgtgggatg cgatggcgca
     8221 tcaccagatg gtcgtcgacg agcagggtaa tctggtggcg gtaggccgat tgtatattaa
     8281 tgccgacaat gaagcgtcca ttcgctttat ggtcgttcac ccggacgtgc aggacaaagg
     8341 gttaggaacg ctgatggcga tgaccctgga gtcggtggcg cgtcaggaag gcgttaagcg
     8401 cgtgacctgt agcgcccgtg aagacgcggt ggagtttttc gccaagctgg ggtttgttaa
     8461 tcagggagaa atcaccacgc caaccaccac gccgattcgc cattttttga tgattaagcc
     8521 cgtcgccact ctggatgata ttttgcatcg cggcgactgg tgcgcgcagc tgcaacaggc
     8581 gtggtacgaa catatcccgc ttagtgaaaa aatgggcgtg cgtattcaac aatataccgg
     8641 gcaaaaattt atcaccacca tgccggaaac cggcaatcag aatccgcacc atacgctgtt
     8701 tgccgggagt ttattctcgc tggcgacgct caccggttgg gggcttatct ggctgatgct
     8761 gcgtgaacgc cacctcggcg gaacgattat tctggcggat gcgcatatcc gctacagcaa
     8821 accgattagc ggtaaacccc atgcggtagc cgaccttggt gccttaagcg gcgatctcga
     8881 ccgtctggcg cgcggacgaa aagcacgggt gcagatgcag gtcgaaatct ttggcgacga
     8941 gacgccgggt gcagtgtttg aaggcacgta tatcgttctg cccgcgaagc catttggccc
     9001 gtatgaagag ggcgggaacg aagaagagta gcttatggag aggggcggtg ctgcctctct
     9061 cattcattcc ggtcatttac atcagtccct gactattccg gttatattga ataagaactt
     9121 tataaatttt tattcttcgt tggcaccata cgtgtcttgc tacatcactg ttcaggagca
     9181 tttaaatatt atttgtagag ctgagtggca attattgcag ataatgctta actcatcata
     9241 ttagttcagg tttgagcgat ttttgatagt caaaaccccc tctggtgcca tacttttttc
     9301 ataaatattt ttaaaatcta tagctcacaa atattatgca aagataatgg gctggcatac
     9361 attgtcagta tcattcattt actgctctgt atgttatgaa tcattatgtt gtatgtttgc
     9421 tcttaaaccg tgggaaggag gctataatgg ttgccaatat taatctcatt aaacaagaaa
     9481 gttattctgt tgttaattta gaaaaacaac taatgagtaa taaatcaccg aagcaaacgc
     9541 tattaaatca a
//
LOCUS       DAZPCS010000142         9537 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZPCS010000142 DAZPCS010000000
VERSION     DAZPCS010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9537
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.143"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(54..533)
                     /gene="xdhC"
                     /locus_tag="ACHUQ7_003230"
     CDS             complement(54..533)
                     /gene="xdhC"
                     /locus_tag="ACHUQ7_003230"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFR1136518.1"
                     /translation="MNHSETITTECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGTVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(530..1408)
                     /gene="xdhB"
                     /locus_tag="ACHUQ7_003231"
     CDS             complement(530..1408)
                     /gene="xdhB"
                     /locus_tag="ACHUQ7_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFR1136519.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATFIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(1419..3716)
                     /gene="xdhA"
                     /locus_tag="ACHUQ7_003232"
                     /pseudo
     CDS             complement(1419..3716)
                     /gene="xdhA"
                     /locus_tag="ACHUQ7_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
     gene            4131..4886
                     /gene="actS"
                     /locus_tag="ACHUQ7_003233"
     CDS             4131..4886
                     /gene="actS"
                     /locus_tag="ACHUQ7_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFR1136520.1"
                     /translation="MSAGRLNKKSLGIVMFLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            4965..5038
                     /locus_tag="ACHUQ7_003234"
     tRNA            4965..5038
                     /locus_tag="ACHUQ7_003234"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4997..4999,aa:Gly,seq:ccc)
     gene            5173..6783
                     /locus_tag="ACHUQ7_003235"
     CDS             5173..6783
                     /locus_tag="ACHUQ7_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1136521.1"
                     /translation="MLKSRTYLYQRNGVFYIRLRMKTTSRLTASLPSHNRYKLASVSL
                     RTKDRRTAMAHSRHIKSALKAIHADNPNASYEELREHLKTIVEWELSVSRDDLNDPES
                     YQLYVDQYDDIKSNLREAVATERLTVDQHRYINDVIGVLKACQDRLNGDSSGLLSYLE
                     PETGSLRPSVSLSVLAEPEVPEPKALTLASLIEQYEQENAQNWKPATLSENRASHSTL
                     IEIFDYLDIQDVGKATRADMLRVREVLQQLPKNRKQRFKSMPLSDLLNRESKTDCLDV
                     VTINNKYLIKMAAVFKWAVRNDLIAKNLTEGLELKVPQRKASDARDAFSPEQVGQLLV
                     AAKAYSQKTSGKPYHYYVTALAAITGARLNEVAQLQVKDVRTTEAGTVFIHINEDDSS
                     LPGKSIKNAHSDRCVPLVDGAYGFVLADFMSLVEDRRKTEGDNAMVFNGLKLMKNGYG
                     EQVSKWFNRTLLPKVLADRSGLAFHSFRHTVATQLKQHGVELAYAQAIMGHSSGSITY
                     DRYAKEVEVETLKEKLAESLSVKKIDGK"
     gene            6815..7234
                     /locus_tag="ACHUQ7_003236"
     CDS             6815..7234
                     /locus_tag="ACHUQ7_003236"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1136522.1"
                     /translation="MVKEFLVSKENKPSYECMNTAETCLGLAGMASEDWQREHHCRTG
                     IIFTAFAIEAMFIFYRKQVDPGYDKTQKECRKTMHKNTLKLCGINNYMGTKPYQIIKE
                     CLEVRDAIAHGDSYTSSFNFSADHLDNQDDIAKVSWR"
     gene            8353..9099
                     /locus_tag="ACHUQ7_003237"
     CDS             8353..9099
                     /locus_tag="ACHUQ7_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFR1136523.1"
                     /translation="MSQQEISIINLDQLVSMTSVEIAELTGKEHKHVLRDIRNMVEEL
                     NGAKTEHCSTLSSELNGSKFGLVGEEVYKDAKGESRTMYRLDRKHTFILVAGYSVHLR
                     AKCYDHIQTLERRVLQLEDQKKRAAIQSANRRGVTWGDYCKTYGLPAQKLMTALLQHR
                     GLFRKNPISNEWSVNPKYSDCFRIIKPSDQKFSAGGYNFRFNAKGLEVFGKPEMVDKM
                     RGILIAFTGTDQQKQEHLLKLAQSGKVEGI"
     gene            9397..>9537
                     /locus_tag="ACHUQ7_003238"
     CDS             9397..>9537
                     /locus_tag="ACHUQ7_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1136524.1"
                     /translation="MMFNNNNWKLSVTDINLYENTVSLDGQSYPLSLAIKTLIPGYLS
                     GLP"
BASE COUNT         2432 a         2362 c         2422 g         2321 t
ORIGIN      
        1 tgttaaacac ataacctcag tgaatccggc ctgaattcag gccggatatc cttttacttc
       61 gttttctcgc aatccagaac tgtatttaca atcatctgat accccgtgca gcgacaaaga
      121 tttcccgcca gtccgcgacg aatttccgta atggttaatg gcttctcgcg tggtttcgcc
      181 agcattgccg tggtagccat aatcaggcca ggcgtacaaa acccgcactg cactgtgccg
      241 gatttcgcat aagcctgctg aacatgagaa agttttccgc ctttcgcttc accttccagc
      301 gtgcggatct cttttccttc agcccaggcg gcaaggtata agcaactgtc tattgctgtg
      361 ccgtcgacca acaccgtaca ggcaccacat tcacccacgc agcacccttg tttgacactt
      421 agcagtcctt gttcgcggag taattccgag agcggcgtgc ctggtgcggc gtgaagctga
      481 aaaggcatcc cgttaatggt gcattcggtg gtaattgttt cgctgtgatt cattgcaatt
      541 ttccccccgc cgcggcgacg gcttcgctaa tcactttttt ggtcatcgtc tggatgagat
      601 gcagacgaaa ctctttactg gcccgccatg aagaacgcgg ggcgacatct tgcaggacag
      661 attcgctgat agcttccagc gtttgcaggt ttaatggcgc attttgtgca gtctgttcgg
      721 catgttggca gcgaatcggc gttggcgcgg caacaccaaa tgccaggcgt aattcgctga
      781 aattgccgtt atccagtcgg caatgtgcgg cgcagccaat cgttgaaata tccattgcgt
      841 cgcgcatggc atatttaaaa tgcgcgctgc ccgcgtgttc tttcggctgt ggcggaaaat
      901 gaaaggcgac gaggatttcg tcatgctcaa gagacacttt gcccggcccg gtgtgaaagc
      961 cattaatcgg gacgaaacga acaccgcgcg gggagtggat ctccagtttc gcgtcataaa
     1021 ttagcgttgg cgtggcagaa tctgcgctgg tggcaccgtt gcaaatattt ccaccgtagg
     1081 tagcgacgtt acggatctgc ggtccagcaa tgaacgtggc cgcagcacat aacgccggga
     1141 gatgacgttg agttatagga tcttctatta gctgggtaaa tgtcgttgca gagccgatac
     1201 gtagcgagcc atcttccgcc agcgtaattc cccgcagctc cgccagatta tgaatatcaa
     1261 caatatggcg ataacggtca ttgtgatggt ggagctgaat cagtacgtca gtgccaccgg
     1321 cgagcagttt ggcctgcggg ttgtcagcca gcaggttgat ggcatcggca agggttgctg
     1381 cgcgatggta agaagcaaaa tcaaacatga tgttatcctc aaatcaatcc tgccagatgg
     1441 aactcttcat ataaccgttt tggcgtcagc ggcagtgtat tgattgcaac accggtagcc
     1501 atcttcaccg cgttacgaat agcagcggca acaggaatta tgggtggctc acccagtgac
     1561 ttatgtccgt atgcggattg cggctcattg atttcgacga acgcgctttc cagttgtggc
     1621 agatccggca tggtcggcat tttgtaatcc agcagattgg ggttacggac cacgccgctt
     1681 ttagcatcga tgatcatctc ttcaaatagc gcccagccaa tgcccattcc cattccgccg
     1741 tgtacctgac cttctgccag cagtggatta agaatatgcc ctgaatcatg aacgttgagg
     1801 atgcggttga tggtgacttt gcacagcgca atatcgaccg tcagatcaac aaaggtacag
     1861 ccaaacgccg gtgggttagt ggtggttttg atggagcttt cagcagagag ctgcccgccg
     1921 cgttcagggt ggtagaaagc gtccatcgcc aaatctttta acgacattaa cggctcttcc
     1981 ggtcgttcaa ccagcacgat atggcctttt atcagggtca aattcatcgc tgactgatgt
     2041 agcatgactg cggcgtgagc gatgattttc tcttttaata atagtgccgc actgcgcagc
     2101 gcaggcgcgg caacatagct ctggcgtgag gcaaatgcgc cgggatcgaa cggcgtaacg
     2161 tcggtatctt gtgttgaaat aacgcgaacg tcgctgaccg gaacccccac ggtttctgcc
     2221 accatttgcg agaagacggt gtcggcaccc tgaccgattt ccgtcgcgcc gctttgcacg
     2281 ttgatggttc catcctgatt catcagaagg cgcgcgcctg ctatttctac gccgacaggc
     2341 caggtgttag aggtgtagct aaaacaggcg acgccaacgc cgcggcgcaa attgccttgc
     2401 tggttctggc attctgcacg gcgtttttcc cattcaaaga ttttccggcc tttttcaaga
     2461 cactccggca accctgcgct gcaaatacgt ttgcccgtga gcggattagc atctccttcg
     2521 cgggcggcgt tgcgtaaacg aatttcaaca ggatcaatac ctaacgctgt cgcggcgtca
     2581 tcaagcatag actcaacggc aaatacgact tgtggcgcgc cataaccacg catcgcacca
     2641 gccgagggga ggttggtata gcaggtcttt gaactgtaag cgtaggcaca acgaggataa
     2701 aggtaagcga ctttattccc ccccgcagaa gcgatggagt gcccgtgaga tgcataagcg
     2761 ccggtgttag acagaacatc cagactataa cctttcaagg ttccgtcgcg gttcacgccc
     2821 atttgcccgt caatagtaaa agcgtggcgg gtacgggttg cgaggaaaca ctcttcacgg
     2881 ctaagggaaa ctttcaccgg aatgccgcca agcttgctgg tcaggaatgc cgccattggc
     2941 tcttccagta catcctgttt attaccaaaa ccgccaccga caaacggttt gatgactcgt
     3001 acgcatgacc agggaatatc cagcgcctga ccaaccacgc ggcgaacaat gtgcgggatc
     3061 tgggtgctgg aaacgatggt aattcgcgag tcatcctcca tccacgccag cgatgttacg
     3121 ctttccatgt gacaatgttg aataacggga gtctgatagt gcccctgtac ctggtagtcg
     3181 gcggcatcga ttgtttgttg gacattgccc gtcgacatcg tgctttgttt cagtaaattg
     3241 ccgccgttat ggattggtgc agcgtcttct gtcagcgccg cttctggcgt ggtgataacg
     3301 ggtaattctt gccactcaat gctgaccaat tgcgccgctt tttctgccgt gagttcatcg
     3361 cgggccacga cgatggcaac ggcgtcacca tgatgacgaa catggcgagt tagcagtgcg
     3421 cgatcggcgg tatcgcgctt gttttcgtca agtgtctagg catgcccagc tgtagcgaat
     3481 ggaatatcag gcacatcttc ccaagtaaaa atcgccagta cgcccggtaa acttctggct
     3541 tgttcatcat taatacttac ggcataacca tgtgcgatag ggctacgtac atatttcgcg
     3601 taacacatgc ccgccataac ataatcgtca gtatatcgtg cccgcccggt gaccttagca
     3661 atggcatcga cgcgcatgca tgattcaccc gtagcggttg cttcccgcgc ttccatagaa
     3721 atcccctcga tttgaacatt tggttcttat ggcaaagatt tagtctaagc aggcaagatt
     3781 tacgccagaa acgacgataa gcgttagggg aggaaggaag tgtgatgacg ttaaaaaaac
     3841 acagatatga tacgaaacag gactctgaat agggacagtg atacgtatca atatgagtgg
     3901 cgataaaatc aatgtgataa atcaggatta ttacgcatca ggatgggaaa tagggtaaaa
     3961 agtcaggaac ttcgatacct ctacagcgaa tattttgctg atgatacttc cctttgccaa
     4021 atgaaattaa cctctttctc tattaccacg tttttccaga agcaagagat tgggtatcaa
     4081 gaggctggct gctatgatat ggcgtcttgt tttttaagcg aggaaagatt ttgagtgcgg
     4141 gacgcctgaa taaaaaatct ctgggtatcg tgatgttttt atcggttgga ctgctattgg
     4201 cgggctgttc gggtagcaaa tcatccgata caggaacgta ttccggctcc gtttacaccg
     4261 tgaaacgggg ggatacgcta tatcgtattt cgcgcaccac gggaaccagc gtaaaagagc
     4321 tggcgcgact gaacggcatt tccccccctt acaccattga agttggtcag aaactaaaac
     4381 tgggtggggc gaaaagtagc agtagtacac gtaaatcaac cgccaaatca acgactaaaa
     4441 ccgcatcggt tacaccgtca tcagcggtac cgaaatcttc ctggccgcca gtagggcaac
     4501 gttgttggtt atggccaacg acagggaaag ttatcatgcc gtattcgaca gcagatggcg
     4561 gcaataaagg gattgatatc tcagctccac ggggtacacc tatttacgcc gcgggtgcag
     4621 gaaaggtggt gtatgtgggc aaccagctgc gtggctacgg taatctcatc atgattaaac
     4681 acagtgaaga ttacattacg gcttacgccc ataatgacac gatgctggta aataatgggc
     4741 aaagcgtgaa ggctgggcaa aaaatcgcca ctatggggag cacggatgcg gcatctgttc
     4801 gcctgcattt ccagattcgt taccgtgcaa cggcaattga tccgctacgt tacttgccgc
     4861 cgcagggcag caagccaaaa tgctgatggc gaattaatca gcagtcagca gcgcggcact
     4921 tgctaaggag agcgtaaggt ttataatgcc ttacgcatct cgaagcgggc gtagttcaat
     4981 ggtagaacga gagcttccca agctctatac gagggttcga ttcccttcgc ccgctccatg
     5041 acacttctta aagtctacta acttactgaa tcctaaggga tacggcttaa cgccttccga
     5101 tagttttact cactgtaacc aacactgtga ctaactttgt tgaagtagtg agcagaccta
     5161 aggtctcagt acatgctcaa gtcaagaacc tacctttatc agcgtaatgg tgtcttttac
     5221 atccgtctta ggatgaagac cactagtcgc ctgaccgctt cgcttccttc tcacaaccgt
     5281 tataaactag cgtcagtatc tttacggacc aaggatagac gtaccgctat ggctcactca
     5341 cggcacatca agtcagcact taaagcaatc catgcagaca accctaacgc ctcttatgag
     5401 gagctacggg agcacctgaa gaccattgtc gagtgggaac ttagtgtcag ccgtgatgac
     5461 ctgaacgacc cggagtccta ccagctttac gttgaccagt acgatgacat taagtccaac
     5521 cttcgggaag ctgtagcgac cgagcgtctg actgtagacc agcatcgcta tattaatgac
     5581 gttatcggtg tgcttaaggc ctgtcaggat agactcaatg gcgatagctc tggcctattg
     5641 tcttaccttg agcctgagac tggcagtcta agaccgtccg tctccctatc tgtattggcg
     5701 gaacctgaag tccctgaacc taaagcactg accctcgcgt ctctcattga gcagtacgag
     5761 caggagaacg cccagaactg gaagccagcg accctaagcg aaaaccgagc gtcacactct
     5821 acgctaatcg agatattcga ctatctggat attcaggatg tcggcaaggc gacccgtgca
     5881 gatatgctca gagttcgtga agtcctccag cagctaccga agaaccgcaa gcaacgcttt
     5941 aagtctatgc cgctgtctga cttgctgaac cgggagtcta agactgactg tctggacgtc
     6001 gttaccatta acaataaata tcttatcaag atggctgctg tgtttaagtg ggcggtacgc
     6061 aacgacttga tagctaagaa cctgaccgaa ggtcttgagc tgaaagtccc tcagcgtaaa
     6121 gcctccgacg ctcgtgatgc gttcagtcct gagcaggtag ggcaactact ggtcgcagct
     6181 aaagcgtact cgcagaagac ctcaggtaag ccataccatt attacgtcac cgctctggct
     6241 gcgattacag gtgcgagact taacgaggtg gcccaacttc aggttaaaga tgtcaggacc
     6301 actgaggctg ggaccgtttt tatccatatc aacgaagacg atagcagcct gccgggtaag
     6361 agtatcaaga acgcccacag tgaccgctgt gtcccgttgg tagatggtgc ttatggtttt
     6421 gtattggctg actttatgtc actcgtggaa gaccgtagaa agactgaggg tgataatgct
     6481 atggtgttca atggcctcaa gctaatgaag aacggctacg gtgagcaggt tagcaagtgg
     6541 tttaaccgaa cgttgctgcc caaagtcctc gctgaccgta gcggcttagc ttttcactcg
     6601 tttagacata cagttgcgac ccagttaaaa caacatgggg tcgagttagc gtatgctcag
     6661 gcgattatgg gtcatagctc agggtctata acttacgacc gttacgcgaa agaggtcgag
     6721 gtggaaacgc tgaaagaaaa attagcggag tcattgtcag taaaaaaaat agacggaaag
     6781 taatcgcata gggatagcgg aatgtgagga cgtaatggtc aaagaatttt tagttagcaa
     6841 agaaaataag ccttcatatg aatgtatgaa tactgctgaa acttgtttgg ggctggctgg
     6901 aatggcttcg gaagattggc aacgtgagca tcattgccgc acaggtataa tatttactgc
     6961 ttttgctatt gaggcaatgt tcatcttcta tagaaaacag gttgaccccg gctacgataa
     7021 gacgcagaaa gagtgcagaa agacaatgca caagaatacg ttaaaattgt gcggtataaa
     7081 caattatatg gggacaaaac cttaccaaat tatcaaagag tgtcttgagg taagagacgc
     7141 tattgctcac ggcgattcgt atacttcgag ctttaatttc tcggcggatc atttggataa
     7201 ccaagacgat attgcaaaag tgtcctggcg gtaaactctg aacaatttcg gggattaact
     7261 gttgagcgcc ttgcaaaata cattgaaatg gcaaagcata ttgaccatga gatttctgat
     7321 aatggttaca ggccttctga aggttacctt ccagcagccg agcgcagaag tctgatgtta
     7381 gcttttggcg tcagtggtgt gtcaatgtgg cccagtgctt gagcgataat acacctcact
     7441 atagaggaat ggacccttaa ctatcactat aggggagaga ccctaagtcc ccaccttaat
     7501 agcagtcacg acccatacac acattcatta agtctcaggc ttccggtagc ggaccgattg
     7561 gaaaccatcc gcagacgcct agtgttaaat caccttggcc tgagaccatt ccttttaacg
     7621 taagctatct tcccttttga caggcgggga gcagtagacc acatccgact ttaagttggc
     7681 tctgtgataa tcgctgcgcg gataactgaa gaaccaagac accattactg aggtctgcca
     7741 tccatttgtg gacaggcgga aactcaaaga ggttagatca gtggtgtctg aggttggagc
     7801 tttgagtctt gaccagcagg ctggaggaga ccatgagttt atcttgttat ggattgtgtt
     7861 atcacaattc caataagaag aactttaagt ccagcctcca ctcccgccaa cccgaagtaa
     7921 aattatggtc gctatgatgt ggtctccatg cttcgggtct tactggaagt actgaacact
     7981 ttcgcgtcaa gttctccgaa gttcattcct gtttgactaa actatcacta tagggaccat
     8041 atctcgcctc tgacctcctg agagacggca agcgagaaac cgtggtccac gctagcaagc
     8101 tagccaccag cctaaggcaa gacagcctct ggtcagcctg tgtgttcttt ggtctcgtga
     8161 cttaagtcta tcaccacaca cgctcggaca tgctgtcgaa ccttcatctc tatggtcatc
     8221 gcagaccact aaccccaatt acctcaagtc attctgtttg cgtcctgttg cctgctggtc
     8281 tccctttacg tgggggcttg agtaggtcag tggggtgcga cttcagggtg caaacaaaag
     8341 gagtacacgt aaatgtccca acaagaaatc agcatcatta acctcgacca gcttgtgtcc
     8401 atgacctcag ttgaaattgc tgagctgaca ggaaaagaac ataaacacgt tttacgagat
     8461 attcgtaata tggtcgaaga gcttaacggg gccaaaactg agcactgctc aactttgagc
     8521 agcgagctta acgggtccaa atttgggctc gttggcgagg aagtatacaa ggacgcgaaa
     8581 ggtgagagcc gaacaatgta tcgtctcgac cgtaagcata ctttcattct ggtcgctggc
     8641 tattccgttc acctccgcgc caagtgttac gaccatattc agacgttaga gcgtcgagtc
     8701 cttcaactgg aagaccagaa gaagcgagca gccattcagt ccgctaaccg ccgaggcgtg
     8761 acttggggcg actactgcaa aacctatggt ctgcccgctc agaagctaat gaccgcactt
     8821 ctccagcatc gtggcctctt tcgtaagaac ccaatcagta acgagtggtc tgtaaatccg
     8881 aagtattccg actgctttcg tatcattaag ccgtcagacc agaagttctc agcaggtggg
     8941 tataacttcc ggttcaacgc taaggggctg gaggtcttcg gtaagcctga gatggttgac
     9001 aagatgcgag gcatccttat tgcattcact ggcaccgacc agcagaagca agagcacctc
     9061 ctgaagctgg cacaatccgg taaggtggag ggaatctaag atgatgattg acccgcgcac
     9121 acctgagggg cgcatgactc tccgttaccg tggctaccgg actgaagtcc tcctcagaga
     9181 gcttggtcta gacccggaag atgagacccg ccagcaccaa tcccgagacg agctaatagc
     9241 ccaactggtc gctatgaaac tgccgcttaa ccgttagtag ctcacctact caatacctaa
     9301 tttaaacacc ttttgagatg accgatgcgg ggagacccgt aggccttact gtgcctgcga
     9361 taccgatagt ctcaactctc aagaggagaa ttacctatga tgttcaacaa caacaactgg
     9421 aagctgtcag tcaccgacat caacctttat gaaaacactg tgagtctgga cggtcagtcc
     9481 tatccgttgt cgctcgctat caagaccctc attccgggct acctgtcagg cttaccg
//
LOCUS       DAZPCS010000143         9389 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZPCS010000143 DAZPCS010000000
VERSION     DAZPCS010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9389
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.144"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            128..1825
                     /gene="maeA"
                     /locus_tag="ACHUQ7_003239"
     CDS             128..1825
                     /gene="maeA"
                     /locus_tag="ACHUQ7_003239"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFR1136525.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            1959..2969
                     /gene="adhP"
                     /locus_tag="ACHUQ7_003240"
     CDS             1959..2969
                     /gene="adhP"
                     /locus_tag="ACHUQ7_003240"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFR1136526.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMEEECIVVADYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMDADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3115..3399
                     /locus_tag="ACHUQ7_003241"
     CDS             3115..3399
                     /locus_tag="ACHUQ7_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFR1136527.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3627..4280)
                     /gene="fdnI"
                     /locus_tag="ACHUQ7_003242"
     CDS             complement(3627..4280)
                     /gene="fdnI"
                     /locus_tag="ACHUQ7_003242"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFR1136528.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4273..5157)
                     /gene="fdnH"
                     /locus_tag="ACHUQ7_003243"
     CDS             complement(4273..5157)
                     /gene="fdnH"
                     /locus_tag="ACHUQ7_003243"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFR1136529.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5170..8217)
                     /gene="fdnG"
                     /locus_tag="ACHUQ7_003244"
     CDS             complement(5170..8217)
                     /gene="fdnG"
                     /locus_tag="ACHUQ7_003244"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(7630..7632),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFR1136530.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPKYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGILRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQSLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQSHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8449..9330
                     /gene="yddG"
                     /locus_tag="ACHUQ7_003245"
     CDS             8449..9330
                     /gene="yddG"
                     /locus_tag="ACHUQ7_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFR1136531.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEIYLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYTAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         2242 a         2480 c         2389 g         2278 t
ORIGIN      
        1 acgattaaac accattgcct gcgcaatggt gtttttatct gctttatact tggggacgac
       61 gccctggcgg taaagcaaag acgataaaag cgtgccaggg atggatattc aaaaaagagt
      121 gagtgacatg gaaccaaaaa caaaaaaaca gcgttcgctt tatatccctt acgctggccc
      181 tgtactgctg gaatttccgt tgttgaataa aggcagtgcc ttcagcatgg aagaacgccg
      241 taacttcaac ctgctggggt tactgccgga agtggtcgaa accatcgaag aacaagcgga
      301 acgagcatgg atccagtatc agggattcaa aaccgaaatc gacaaacaca tctacctgcg
      361 taacatccag gacaccaacg aaaccctctt ctaccgtctg gtaaacaatc atcttgatga
      421 gatgatgcct gttatttata ccccaaccgt cggcgcagcc tgtgagcgtt tttctgagat
      481 ctaccgccgt tcacgcggcg tgtttatctc ttaccagaac cgccacaata tggacgatat
      541 tctgcaaaac gtgccgaacc ataatattaa agtgattgtg gtgactgacg gtgaacgtat
      601 tctggggctt ggtgaccagg gcatcggcgg gatgggcatt ccgatcggta aactgtcgct
      661 ctataccgcc tgtggcggca tcagcccggc gtataccctt ccggtggtgc tggatgtcgg
      721 aacgaacaac caacagctgc ttaacgatcc gctgtatatg ggctggcgta atccgcgtat
      781 cactgacgat gaatactatg aattcgttga tgaatttatc caggctgtga aacaacgctg
      841 gccggacgtg ctgttgcagt ttgaagactt tgctcaaaaa aatgcgatgc cgttacttaa
      901 ccgctatcgc aatgaaattt gttcttttaa cgatgacatt cagggcactg cggcggtaac
      961 agtcggcaca ctgatcgcag ccagccgcgc ggcaggtggt cagttaagcg agaaaaaaat
     1021 cgtcttcctt ggcgcaggtt cagcgggatg cggcattgcc gaaatgatca tcgcccagac
     1081 ccagcgcgaa ggattaagcg aggaagcagc gcggcagaaa gtctttatgg tcgatcgctt
     1141 tggcctgctg acggacaaga tgccgaacct gctgcctttc cagaccaaac tggtgcagaa
     1201 gcgcgaaaac ctcagtgact gggataccga cagcgatgtg ttgtcactgc tggatgtggt
     1261 gcgcaatgta aaaccagata ttctgattgg cgtctcagga cagaccgggc tgtttacgga
     1321 agagatcatc cgtgagatgc ataaacactg tccgcgtccg atcgtgatgc cgctgtctaa
     1381 cccgacgtct cgcgtggaag ccacaccgca ggacattatc gcctggaccg aaggtaacgc
     1441 gctggtcgcc actggcagcc cgtttaatcc tgtggtatgg aaagataaaa tctaccctat
     1501 cgcccagtgt aacaacgcct ttattttccc gggcatcggc ctgggtgtta ttgcttccgg
     1561 cgcgtcacgt atcaccgatg agatgctgat gtcggcaagt gaaacgcttg ctcagtattc
     1621 gccgctggtc ctgaacggcg aaggtctggt actaccggaa ctgaaagata ttcagaaagt
     1681 ctcccgcgca attgcgtttg cggttggcaa aatggcgcag cagcaaggcg tggcggtcaa
     1741 aacgtctgct gaagctttgc aacaagccat tgacgataat ttctggcaag ccgaataccg
     1801 cgactaccgc cgtacctcca tctaagcctg cacccggtag tgaaggctac cgggctattt
     1861 ccctctccct ttttcagatc tcatccatac tgggtagtgg cgaataaatc tcatttgcct
     1921 cacctgctat gcagaacatc atccgaaaag gaggaactat gaagactgca gttgttacga
     1981 aggatcatca tgttgacgtt acggataaaa cactgcgctc actgaaacat ggcgaagccc
     2041 tgctgaaaat ggagtgttgt ggtgtatgtc ataccgatct tcatgttaag aatggcgatt
     2101 ttggtgacaa aaccggcgta attctgggcc atgaaggcat cggtgtggtg gcagaagttg
     2161 gcccaggtgt cacctcatta aaaccaggcg atcgtgccag cgtggcgtgg ttttacgaag
     2221 gatgcggtca ttgcgaatac tgtaacagtg gtaacgaaac gctctgccgt tcagttaaaa
     2281 atgccggata cagcgttgat ggcgggatgg aggaagagtg catcgtggtc gccgattacg
     2341 cggtaaaagt gccagatggt ctggactcgg cggcgcccag cagcattacc tgtgcggggg
     2401 tcaccaccta caaagccgtt aaactgtcaa aaattcgtcc cggtcagtgg attgctatct
     2461 acggtcttgg cggtctgggt aacctcgccc tgcaatacgc aaagaatgtc tttaacgcca
     2521 aagtgatcgc cattgatgtc aatgatgagc agttaaaact ggcaaccgaa atggatgcag
     2581 atttagcgat taactcacgc accgaagacg ccgccaaaat tgtgcaggag aaaaccggtg
     2641 gcgctcacgc tgcggtggta acagcagtag ctaaagctgc gtttaactcg gcagttgatg
     2701 ctgtccgtgc aggcggtcgt gttgtggctg tcggtctgcc gccggagtct atgagcctgg
     2761 atatcccacg tcttgtgctg gatggcattg aagtggtcgg ttcgctggtc ggcacgcgcc
     2821 aggatctaac cgaagccttc cagtttgccg ccgaaggcaa agtggtgccg aaagtcgccc
     2881 tgcgtccgtt agcggacatc aacaccatct ttaccgagat ggaagaaggc aaaatccgtg
     2941 gccgtatggt gattgatttc cgtcactaag aggtctttgc tgcgactccc atgttcgggt
     3001 cgcagcatcg cacactctcc aacatgaaat ggctaaatgg attattgttg cgtaggaagg
     3061 aacaataatg atagtgttcc cagagaagta acgctactga gagtaaagac actaatgaaa
     3121 atggccaatc atccccgccc gggggacatt attcaggaat cactggacga acttaatgtc
     3181 agcctgcgcg agtttgccag agcaatggaa attgcgccct caacggcaag ccgattgctg
     3241 accggaaaag cagctttgac gccagaaatg gcaattaaac tttccgtggt gatcggcagt
     3301 tcgccgcaaa tgtggctgaa tctgcaaaac gcctggagtc tggcagaggc agaaaaaacg
     3361 gtggttgtgt cgcgattacg ccgtctggtt acgcaataaa aaatggcacc ctataaagcg
     3421 ccatttgaaa ctgatgacaa acgcaacatt gcctgatgcg ctacgcttat caggcctaca
     3481 cgttcacttc aatctattgt atttgaaggg ttttcatgcc ggataaggca ttcacgacgc
     3541 atccgggatg aacaaagccc acgttgtcta caatatgaaa atggcgcctt ttacagcgcc
     3601 attttcagct taaagttcaa aagagattat atcccttctt cactctcttt tttcgcttct
     3661 gccttctcga tttcacgata ccagcgcgga tggtgtttct tcgcccagcg acggctcacc
     3721 ttcccttcga tcatcccttt aatcgaccct ttcacccaga atgccatata catatggatc
     3781 aggatggcgt ggatcaggat gatacccgca gccgcgtgga tcagcaggct gtagcgaaca
     3841 acctgcatcg ggaagtactg cgcaaagtac ggacgccaga taatcacccc ggtcaccagc
     3901 agcacgaaaa tcatgctcat gatcgaccag aacatcatct tttgcccggc gttgtactta
     3961 ccaacatccg ccactttgtg ctcattgcct tttaatactt cgacaatgtt caacagccac
     4021 ggaatatctt tcttatccgg gatgttgtga tgcacaaaac gcacaaacat aaacatcagt
     4081 gcgacgaaaa tcgcaatgcc gaagaagggg tgcaaaatgc gtcccatctg cggcgtaccg
     4141 aaggtttgcg tcagccattg cagcgtcggg aagaagaacg aaatcccgga cagcgccacc
     4201 aggaagaagc aaatcaccac ggtccagtga caggcgcgat caataaattt ggtgcgcaca
     4261 atcattttcg acttactcat gatgatcctc ctcgtcatcg tccacttcct tattcgggcc
     4321 aataccgatg tagtggaaaa tcaacccggc aaaagtggca ataaagccag ccgctgccag
     4381 cggtttcaac gcgcctttcc acaggcttac cgaggtgtcg atcttcggat ctttcggcag
     4441 accgtgatac agctccggct gatcggcgtg atgcagcacg tacataacgt gcgtaccacc
     4501 gaccccttcc gggttgtaga cgccagcatg ttcgtaacca cgcgctttca gtttcgccac
     4561 gcgctgttcc gccagctcca gcatctcctt cttggtgccg aagtggatag ccccggtcgg
     4621 acaggttttc acacaagccg gttcctggcc gacgctgacg cgatcgacgc agagcgtgca
     4681 tttatatacc cggttatcct ctttgttgag gcgcggaata ttaaacggac acccggcaat
     4741 gcagtaacca cagccgatgc agttttccga ctggaaatcg acaatcccgt tagcgtactg
     4801 aatgattgca ccagcagacg ggcacgcctt caggcagccc ggatcttcac agtgcataca
     4861 gccgtcttta cggatcagcc actccagctt gccattctgt tcggtttcgc taaagcgcat
     4921 caccgtccag gacttggcgc tcagatcggc ggggttatcg taaaccccga cgcagtgccc
     4981 cacttcatca cggatgtcgt tccactccga acacgccacc tgacaggctt tacagccgat
     5041 acaggtggaa acgtcgataa gtttcgcgac ttctgctttg taatcacgca cctgagaagg
     5101 cggcgtgatg gagttagttg cggacctttt gataatatcc tgcgtttcca tagccatctg
     5161 ttcgccccct tacgccttct cgatgttgac taagaacgct ttatattccg gcgtttgcga
     5221 gtttgcatca ccgacattcg gcgtcagagt gttagcgata taacctttac gcgcgacacc
     5281 ctcaaagccc cagtggattg gaatacccac cgtttcaacc tgctgaccgt ttacattcag
     5341 cggtttcaga cggcgcgtta ccactgccac cgcgcggata aagcctcgct tgctggagac
     5401 agtgacacga tcgccattat taatgccttt cgccgccgcc tgcgtttcgc tgatttccac
     5461 aaactgttcc ggctgagcaa ttgcgttgag caacgcgtgc ttggtccagg tgtggaagtg
     5521 ctcggtcaga cgataggtcg tacccacata cgggaactgc tcttttttac ccatccgcag
     5581 cgcgtcttgt tcatacagac gaacaaccgg gttagacacc acgttcggat gcagcgggtt
     5641 agtgcccagc ggcgtttcaa tcggctcgta gtgttccggg aacggacctt ccgccatttt
     5701 gttgatggca aacaggcgtc ccatcccttc cggctgcata ataaacggcc cggttggcgt
     5761 acccggtgcg gcattgccga agtccggaat atcgttaccc gtccacttgc tgccgttcca
     5821 ctggatcagc atccgtttcg gatcccacgg tttaccgttg atatccgccg aagcacggtt
     5881 gtagagtacg cgacggttga gcggccacgc ccaggcccat cccagcgtat tccccagacc
     5941 ggacgggtcg gagttatcgc ggtttgccat ctggttaccc tgctctgtcc agctaccggt
     6001 gtagatccag caagaagatg cggttgtacc gtcatcacgc agatgcgcaa agctactcag
     6061 caactgacct ttcttcgcaa tcagcacgcc attagcatca tagagatctt ccaacgcata
     6121 gccgttgttc tctttagcta cttcgtcaga ttgcggttcg tgcggctgct tgtagttcca
     6181 gctcatcttc atcagcggtt ctacgccttt accaccttcg gcctggtaca actcgcgcag
     6241 atgatggtag atacccgcca gaatttcgcc gtcgttacgc gcttcgcccg gcgcgtcctg
     6301 acctttccag tgccactgca accagcgacc ggagttagcg atagaaccat cctcttcagc
     6361 aaagcaggtc gaaggcagac ggaatacttc agtctgaata gacgccggat cgacatcgtt
     6421 cgactcgccg tggctctgcc agaaggtaga ggtttcagtc accagcggat cgataaccac
     6481 catgtacttc agtttgctca ggcagctcac cactttgttt ttatccggga aggacgcaac
     6541 cgggttaaag ccctggcaga aataaccggt aactttgcct tcatccatca tgttgaaata
     6601 cttgatgacg tcgtaggtct ggtcccactt cggcagccag tcatagcccc agttgttctc
     6661 tttctgcgcg gcatcgccat agaaagattt catcaggcta acgaagaact tcggatagtt
     6721 gctccagtag ttcacctgat cagccagcgt cgctttcggc gtgttcgctt ccagatacga
     6781 ctgcaaatca acctgttttt ctgacggcag cgtcagataa cctggcaggc tggtagagag
     6841 cagacctaag tcagtcagcc cctgaatgtt ggagtgacca cgcaatgcgt tcacgccgcc
     6901 accggccata cccatgttac cgagcagcga ctggatcatc gccatagtac ggatgttctg
     6961 cgcacccacg gtgtgctgcg tccagcccag cgcgtacagg aaggtggttg tgcgatccgg
     7021 tgcgctggtg gaggccagca cttcacacac tttcaggaag tcggcttttg gcgtaccaca
     7081 gatgttttca acaacgtccg gcgtgtagcg ggaaacgtgc gctttcagca ggttccacac
     7141 acagcgcgga tgagtcagtg tttcatcgcg tttcgcatag ccgttttcat cgaactgata
     7201 gttccaggac gatttgtcgt actggcgttt ttcagcgtca taaccgctga acaggccatc
     7261 ttcgaaagca aaatcatcac gcaccagtag gctggcgttg gtgtaatgtt taacgtattc
     7321 ggcgttgatt ttgttgtttt cgatcaggta gcgcaaaatg ccagacagga acgtaatgtc
     7381 cgtaccggaa cgaataggtg cgtaaatatc cgccacagaa gcggtacgcg taaaacgggg
     7441 atcgacaacg atcaaggttg catcgttgtt gtttttcgct tccatcgccc agcggaaacc
     7501 gacgggatgc gcttcagcag cgttaccgcc cattaccatt acgacgttag cgtttttgat
     7561 atccacccag tggttggtca tagcaccgcg accaaatgtt ggagcaagac ttgctaccgt
     7621 tggtccgtgt cagacgcgcg cctggttgtc taccgccagc atcccgaggg agcgggcaaa
     7681 tttctgggtc agcatcccgg tttcgttgct ggcaccggag gcacacagca taccggtaga
     7741 aagccaacgg tttaccgtta cgccctgctc gttcttttca ataaagttag cgtcacggtc
     7801 agctttcatc agcttcgcaa tacgggagaa tgcttcttcc cagctaatgc gctgccattt
     7861 gtcagaacct ggcgcacgat atttcgggta gcgcagacgg ttttcactgt tgacgtaatc
     7921 cagcaaaccg gcccctttcg ggcacagcgc accacggctt accggatgat ccgggtcacc
     7981 ttcaatgtga taaatcgctt ctctggcgtt ttttgcgcca tcacccaggc tatacatcaa
     8041 tagcccgcaa cctacggaac agtatgtgca ggtgttacgg atctctttag cgcgtaataa
     8101 tttgtagttt cgcgcctgag ccagtgcttg cttcggggca aagcccaatg ccgctaccgt
     8161 tgttccagcc ataccgcccg cgcagatttt aaaaaattgt ctgcgactga cgtccattgc
     8221 tttcctcttt tttcaggggt attactgcgc gaggaaacta acagtaaaat tctgaatctt
     8281 tttgcgcgaa atcaaaaaga acacgtgttt gccacaaaat aatccgcctg cgcaaattga
     8341 cctacctcaa tagcggtaga aaaacgcacc actgcctgac aggccagtta aaaaaatgct
     8401 ataaaattca gcttaatttt taacggcaag agagacaaaa cagcgagcat gacacgacaa
     8461 aaagcaacgc tcatagggct aatagcgatc gtcctgtgga gcacgatggt aggattgatt
     8521 cgcggtgtca gcgaggggct cggcccggtc ggcggcgcag ctgctatcta ttcattaagc
     8581 gggctgctgt taatcttcac ggttggattt ccgcgtattc ggcagattcc gaaaggctat
     8641 ttactcgccg ggagtctgtt attcgtcagc tatgaaatct atctggcgct ttccttaggg
     8701 tatgcggcga cccgtcatca ggcgattgaa gtgggtatgg tgaactatct gtggcccagc
     8761 ctgacaattc tctttgccat tctgtttaat ggtcagaaaa ccaactggct gattgtacca
     8821 ggattattat tagccctggt cggcgtctgc tgggtgttag gcggtgagaa tgggttacat
     8881 tatgatgaaa tcatcaataa tatcaccacc agcccattga gttatttcct ggcgtttatt
     8941 ggtgcgttta tctgggcagc ctattgcaca gtaacgaata aatacgcacg cggatttaac
     9001 ggaattaccg tttttgtcct gctaacggga gcatgtctgt ggatttacta ttttctcacg
     9061 ccacaaccag aaatggtatt tagcacgccc gtcatgatta aactcatctc tgcggcattt
     9121 accttaggat ttgcttatac tgcatggaat gtcggtatat tgcatggcaa tgtcaccata
     9181 atggcggtag gctcgtattt tacgcctgta ctttcctcag cgcttgcagc cgtgctgctc
     9241 agcgccccgc tgtcgttctc gttctggcaa ggcgcactaa tggtctgcgg cggttccctg
     9301 ctctgctggc tggcgacacg tcgtggttaa aataattgcc gggtctacgg cccggcagtt
     9361 aatcagataa attccgacaa tattaaaaa
//
LOCUS       DAZPCS010000144         9328 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZPCS010000144 DAZPCS010000000
VERSION     DAZPCS010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9328
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.145"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..256
                     /gene="mliC"
                     /locus_tag="ACHUQ7_003246"
     CDS             <1..256
                     /gene="mliC"
                     /locus_tag="ACHUQ7_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFR1136532.1"
                     /translation="ERMQTDTLEYQCDEKPLTVKLNNPRQEVSFVYDNQLLHLKQGLS
                     ASGARYSDGIYVFWSKGEEATVYKRDRIVLNNCQLQNPQR"
     gene            315..971
                     /gene="pdxH"
                     /locus_tag="ACHUQ7_003247"
     CDS             315..971
                     /gene="pdxH"
                     /locus_tag="ACHUQ7_003247"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFR1136533.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            1100..2374
                     /gene="tyrS"
                     /locus_tag="ACHUQ7_003248"
     CDS             1100..2374
                     /gene="tyrS"
                     /locus_tag="ACHUQ7_003248"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFR1136534.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            2433..3296
                     /gene="pdxY"
                     /locus_tag="ACHUQ7_003249"
     CDS             2433..3296
                     /gene="pdxY"
                     /locus_tag="ACHUQ7_003249"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HFR1136535.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIEQGPQIVLVKHLARAGYSRDRFEMLLVTADEVWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(3340..3945)
                     /gene="gstA"
                     /locus_tag="ACHUQ7_003250"
     CDS             complement(3340..3945)
                     /gene="gstA"
                     /locus_tag="ACHUQ7_003250"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFR1136536.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     SVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(4051..5553)
                     /gene="dtpA"
                     /locus_tag="ACHUQ7_003251"
     CDS             complement(4051..5553)
                     /gene="dtpA"
                     /locus_tag="ACHUQ7_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFR1136537.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKATVA"
     gene            complement(6165..6800)
                     /gene="nth"
                     /locus_tag="ACHUQ7_003252"
     CDS             complement(6165..6800)
                     /gene="nth"
                     /locus_tag="ACHUQ7_003252"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFR1136538.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     ISVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(6800..7495)
                     /gene="rsxE"
                     /locus_tag="ACHUQ7_003253"
     CDS             complement(6800..7495)
                     /gene="rsxE"
                     /locus_tag="ACHUQ7_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFR1136539.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(7499..8119)
                     /gene="rsxG"
                     /locus_tag="ACHUQ7_003254"
     CDS             complement(7499..8119)
                     /gene="rsxG"
                     /locus_tag="ACHUQ7_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFR1136540.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKT
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWLTHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(8123..9181)
                     /gene="rsxD"
                     /locus_tag="ACHUQ7_003255"
     CDS             complement(8123..9181)
                     /gene="rsxD"
                     /locus_tag="ACHUQ7_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFR1136541.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLRFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(9193..>9328)
                     /locus_tag="ACHUQ7_003256"
     CDS             complement(9193..>9328)
                     /locus_tag="ACHUQ7_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001634432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="electron transporter RnfC"
                     /protein_id="HFR1136542.1"
                     /translation="APKHASWNSNRLMRNQKNRSIRAKPQLPRLLPAFRPKKPPSKRL
                     "
BASE COUNT         2386 a         2336 c         2369 g         2237 t
ORIGIN      
        1 tgagcgaatg caaaccgata cgctggaata ccagtgtgat gaaaaaccgt tgacggtcaa
       61 actgaataat ccgcgccagg aagtcagttt tgtttacgat aatcaactac tgcatctcaa
      121 acagggcctt tcagcctctg gcgcgcgtta ctccgacggg atctatgttt tctggtcgaa
      181 aggtgaggaa gcgactgtct ataaacgcga ccgcatcgtc ttgaataact gtcagttaca
      241 aaatccacag cgttgagatt tttccagggg cggcgcacaa tagcgtcacc cactgacaat
      301 ccgtaaagaa aaccatgtct gataacgacg aattgcagca aatcgcgcat ctgcgccgtg
      361 aatacaccaa aggcgggtta cgccgccgcg atcttcccgc cgatccatta accctttttg
      421 aacgttggct ctctcaggct tgtgaagcca aactggcgga ccctaccgcg atggtggtcg
      481 ctaccgtgga tgaacatggt cagccttatc agcgcatcgt tttactcaaa cattacgacg
      541 aaaaaggcat ggtgttttac accaacctcg gtagccgtaa agcacatcaa attgaaaata
      601 atccgcgcgt tagcctgctg ttcccgtggc atacccttga acgccaggtg atggtgatcg
      661 gtaaagcaga acgactttcg actctcgaag tgatgaaata ttttcatagc cgcccgcgtg
      721 atagccagat tggtgcatgg gtttcgaagc agtccagtcg catttctgcc cgcggtatcc
      781 ttgaaagtaa attcctggag ctgaagcaga agtttcaaca gggcgaagtg ccattgccga
      841 gcttttgggg cggttttcgc gtcagccttg aacagattga gttctggcag ggcggtgagc
      901 atcgcctgca tgaccgcttt ttgtaccagc gtgaaaatga tgcgtggaag attgatcgtc
      961 ttgcaccctg aaaagatgca aaaatcttgc tttaatcgct ggtactcctg attctggcac
     1021 tttattctat gtctctttcg catctggcga aaagtcgtgt accggcaaag gtgcagtcgt
     1081 tatatacatg gagattttga tggcaagcag taacttgatt aaacaattgc aagagcgggg
     1141 gctggtagcc caggtgacgg acgaggaagc gttagcagag cgactggcgc aaggcccgat
     1201 cgcgctctat tgcggcttcg atcctaccgc tgacagcttg catttggggc atcttgttcc
     1261 attgttatgc ctgaaacgct tccagcaggc gggccacaag ccggttgcgc tggtaggcgg
     1321 cgcgacgggt ctgattggcg acccgagctt caaagctgcc gagcgtaagc tgaacaccga
     1381 agaaactgtt caggagtggg tggacaaaat ccgtaagcag gttgccccgt tcctcgattt
     1441 cgactgtgga gaaaactctg ctatcgcggc gaacaactat gactggttcg gcaatatgaa
     1501 tgtgctgacc ttcctgcgcg atattggcaa acacttctcc gttaaccaga tgatcaacaa
     1561 agaagcggtt aagcagcgtc tcaaccgtga agatcagggg atttcgttca ctgagttttc
     1621 ctacaacctg ttgcagggtt atgacttcgc ctgtctgaac aaacagtacg atgtggtgct
     1681 gcaaattggt ggttctgacc agtggggtaa catcacttct ggtatcgacc tgacccgtcg
     1741 tctgcatcag aatcaggtgt ttggcctgac cgttccgctg atcaccaaag cagacggcac
     1801 caaatttggt aaaactgaag gcggcgcagt ctggttggat ccgaagaaaa ccagcccgta
     1861 caaattctac cagttctgga tcaacactgc ggatgccgac gtttaccgct tcctgaagtt
     1921 cttcaccttt atgagcattg aagagatcaa cgccctagaa gaagaagata aaaacagcgg
     1981 taaagcaccg cgcgcccagt atgtactggc ggagcaggtg actcgtctgg ttcacggtga
     2041 agaaggttta caggcggcaa aacgtattac cgaatgcctg ttcagcggtt ctttgagtgc
     2101 gctgagtgaa gcggacttcg aacagctggc gcaggacggc gtaccgatgg ttgagatgga
     2161 aaagggcgca gacctgatgc aggcgctggt cgattctgaa ctgcaacctt cccgtggtca
     2221 ggcgcgtaaa actatcgcct ccaatgccat caccattaac ggtgaaaaac agtccgatcc
     2281 tgaatacttc tttaaagaag aagatcgtct gtttggtcgt tttaccttac tgcgtcgcgg
     2341 caaaaagaat tactgtctga tttgctggaa ataatgcatt aagtggaaag ggggagtgag
     2401 aaatcactcc ccctggtttt tatacaggga acatgatgaa aaatattctc gctatccagt
     2461 ctcacgttgt ttatggtcat gcgggtaaca gtgcggcaga gtttccgatg cgccgcctgg
     2521 gcgcgaacgt ctggccgctg aacaccgttc aattttctaa tcacacccaa tacggcaaat
     2581 ggactggctg cgtgatgccg cccagccatt taaccgaaat tgtgcaaggc attgccgcca
     2641 ttgataaatt acacacctgt gatgccgtat taagtggcta tctgggatcg gcggagcagg
     2701 gtgaacatat cctcggtatc gtccgtcagg tgaaagcagc gaatccgcag gcgaaatatt
     2761 tttgcgatcc ggtaatgggt catccggaaa aaggctgtat cgttgcaccg ggtgtcgcag
     2821 agtttcatgt gcggcacggt ttgcctgcca gcgatatcat tgcgccgaat ctggttgagc
     2881 tggaaatact ctgtgagcat gcggtaaata acgtcgaaga agcggttctg gcagcgcgcg
     2941 aactcattga gcaagggcca caaattgtgt tggttaaaca cctggcgcga gctggctaca
     3001 gccgtgaccg ttttgaaatg ctgctggtca ccgccgatga agtctggcat atcagccgtc
     3061 cactggtgga ttttggtatg cgccagccgg taggtgttgg tgatgtgacg agcggtttac
     3121 tgctggtgaa actgcttcag ggggcaacgc tgcaggaggc cctggaacat gtgaccgctg
     3181 cagtctacga aatcatggtg accaccaaag caatgcagga atatgagctg caagtggtgg
     3241 ctgctcagga tcgtattgcc aaaccagaac attacttcag cgcaacaaag ctctgaaata
     3301 tctcatttcg gcccacaacg acctgtgggc cgatggctat tactttaagc cttccgctga
     3361 cagcgcgtct tgtacttccg gacgttcagc catacgttgc ataaatgccg caatgtgctc
     3421 taacccttcc agattcagtt tcaccgcgta tgcccagcgc agaaccgtaa acagataggc
     3481 atcagcaatt gtaaatcttt gcccgcagat ccagtgctca tccttcagtg cctcgttcac
     3541 atattgcagc ttcttatcca gctgcgcgcg aactgtcggt ttgtactctt ccggtgtatc
     3601 tgggcgaaac agaggtgtga aacctttatg cagctcggtg gcgatgtaat tcagccattc
     3661 gatggtttta tagcgggaaa tactgtttac cggtgccagc aactggcggt cggggacgct
     3721 gtcggcaaga tactgcataa tcgcaacgcc ttccgtcagc aaagtaccat cgtccagcag
     3781 caatgcaggc acctgcccct tagggttaac ggaaaagtaa tcgtcaccgt tttcgagacg
     3841 ttttttcatt aaatccacac tgacgagcgt aaaatccttt ccgctctcac gcagggtgat
     3901 atgggaagcg agagagcagg cgcccggttt gtagaacaat ttcatcggta actccttttg
     3961 tctgtggttt aaagtatggt agtgcgttgc tgaacaaaaa aaaagccgct aacgaaaagt
     4021 tagcggcttg taaaagagtt tccctgaatt ttacgctaca gtagctttcg ccgctttgtc
     4081 tgcagcgtca tcctgcgtca tgcggtgcag tttaggcgcg gtcagcagca tcagtactgc
     4141 aataacggca gtagcgacac caatctgcaa gaatacgcga ccatagactt ccagtgacat
     4201 cagcgggtcg gtaacgttat ccggcacagc catcataccc gcaacataac caccaatcag
     4261 gtttgcaccg gcagtggtca ggaaccagct acccataatg aagcccatca gacgctgcgg
     4321 aacgagttgt gcaaccattg ccagacccag accagagatc atcagttccc cgatgctctg
     4381 caggccatag cttgcgacca gccagctgac agacacgata cctgcgtcag acgcgaattt
     4441 cgcacccagc ggcagaatca ggaacgcacc agaacacatc accatgccga ttgcaaactt
     4501 ggttggcatc ggcagggtat cacccatctt gttatagata gcggccagaa tcggactacc
     4561 gatgatgatc cagaacgggt tcagtgcctg atactgctca ggttctacgg ccagacccag
     4621 aatggagtgc tcaacgttac gaatcgcaaa gaagttcagt gacgttggca tctggctgta
     4681 cagcacgaag aagatgattg cttcgagcat caggatgaag gcaacgatca ttttacgacg
     4741 cgcagcacct ttcatcgcga aagcttcttt accgaagata accacaatac cgaaggcaac
     4801 aacgcccagc gccatacgcg caacttcctg attgtgcagt agccaggtgg caacagcgat
     4861 cagcgccaca acaccaataa tggtcagcaa caggttacgg tagttgactg gttcaaagtc
     4921 tggttttgaa ccgtactgtt taacccagcg ttggcagaag gcgaagttaa cgatagtgat
     4981 cagcaggcct acaacgctca acgcaaacgc aacactccag ccgtatttcg cggccagcca
     5041 cggggtggct atcatagaga agaaagagcc gatgttgacg gacatgtagt acatggtgaa
     5101 tgcaccgtcc agacgtgggt cgtttttctc atagcatgta gaaagcagag aagacgggtt
     5161 agctttaaac aggccgttac cgaccgcaat agccgccata cccatataaa caataccggc
     5221 gtcgtgacca gaccaggcaa ccagagcata accaatcgcc agcacaatag cgccgagcat
     5281 aattacgcgt ttagtaccca gtaccttgtc acctaaccag ccgccgatag cgaccagacc
     5341 ataaaccagg gcactaaagg aagagaaaag ggtgattgaa tccgcttcag acatacccag
     5401 ttgtttaacc aggtaaacag ccataattcc ttgtaggccg taataaccaa aacgttccca
     5461 taactcaatc gagaagatga gatagaacgc cttcggttgt ttgaaagcgt tcaaactgac
     5521 gctttcagtt ggtttttggt ttgcagtgga cacttttacc tctttttttt acatcccata
     5581 ttaacggggg tgctttctcg cgttatgtct aaaaaacata cgcgcaattg ttatagtggg
     5641 aggggaaacg gcaggtaatg ttcactatct tgcactttca gacaagtcct ttgtaataat
     5701 ctgttacata taactgaggc ggggaattca accattccgg gaaatgttat ccagcgttaa
     5761 atttttcaaa aaacgtaacg gcagattttt tcactgcatt gagaggggga tgatggcttg
     5821 gcggcgatat gttctgctat ttagcgtttt tcctagtgag atagtccagt ttctgaaaaa
     5881 tagccagtgt aatgttttgc aggtcaatat ttgttgcgtg tgtaaattta aatcatttta
     5941 acctgattct tgcaatgttt ttacattgcc acagcatcgt gatcttgatc acgaatgaat
     6001 agaaaatttc gctattaaaa aaacaattaa cctgcatttt tggttatccg caaacggatt
     6061 acctgatgat aacgctaagc aatggcatca ttgacgttaa aaagatgttt taaaagagga
     6121 taaagaaagg ttatcaatgg ggtaatcggt gttacccctt ttcttcagat gtcaactttc
     6181 tctttgtatt cacaaagatc ttcaataata caagagccac agcggggctt gcgggcaatg
     6241 caggtataac gcccgtgcag gatcaaccaa tggtggcagt cgactttaaa ctctgccgga
     6301 accactttca gtagcttttc ttctacctgt tcgacgtttt tccccggcgc aaattgagta
     6361 cgattacaaa cgcggaaaat gtgcgtgtcg acagcaatag tcggccagcc gaatgcagtg
     6421 tttaatacga cgttggctgt tttacgacct acgccgggca gggcttcaag cgcagcacga
     6481 tcttccggaa cctcgccatt atgttgttcc agtaatatac ggcaggtttt gatgatattt
     6541 tctgctttgc tgttataaag gccaatcgtt ttgatatagg ttttcacccc ttcaacgcct
     6601 agttcaagca tcgctgcagg cgtattcgcc accgggtaga gtttcgccgt cgccttatta
     6661 acactgatat cggtcgcctg agcggaaagc agtacggcaa tcagcaattc aaaaggcgaa
     6721 ctgaaattaa gctcggtggt gggatgagga ttgttctcac gcaggcgagt gaggatctcc
     6781 agacgttttg ctttgttcat cagacattcc ctgtttcacc gtttggcaat gcacgttcgg
     6841 cagctgcttc agcacggcgc tttttcattc tttcatcaat caggtatttt cctgccagca
     6901 tcagtcccaa gccaataaat gcacctggtg gcagcatcgc cagcaggaaa ggggagtcgg
     6961 tgtggaaaat ctccacgcgt aatacttttg cccagctacc taacagcgca tctgcaccgt
     7021 caaacaacgt gccattgccg ataatttcgc gtagtgaacc cagcacgaac atggcgcagg
     7081 ttgcgcccgt accaattgaa aagccgtcca gtgccgaaag cgccggacct tttttggcgg
     7141 cgaaggcttc agcgcggccc acaacgatac agttagtgac aatcagcgga ataaaaatcc
     7201 ctaatgattg atacaggcca aaggcgtagg cgttgatcag catctgtaca gcgctgacca
     7261 ccgaggcgat gatcatcacg taaatgggaa tgcggatctc ggctggcgtc cagtgacgca
     7321 gcgtcgaaat ggtcaggttg gtcagcgtca gtaccagcgt agtcgcaagt cctaaaccca
     7381 gagcgttagt ggcagtggat gtgaccgcca atagcggaca aaggccgagc agttggacca
     7441 gtgcagagtt gtttttccac aacccctgaa caataacgtc tttaatttcg ctcacgcttt
     7501 attctccaca ggcaggaagt tgagaaagtt gtgccggtaa cgtctgagcg tacaatccgg
     7561 cgcgttttac cgcattaacc accgcgcggg gagtaatcgt cgcgccggtg aactggtcga
     7621 aatcaccacc atctttcttc accgcccagt gcgcatcatc cgcaccactg attttttttc
     7681 ccgcaaaatg ggtgagccag tcagaaaggc gcaattcgat tttatcgcca agccctggcg
     7741 tttcgtggtg ctctgtcacg cgcgtgccaa gtaccgtgcc attaaaatcg gctcccacca
     7801 gcagctgaat cgcaccggaa tagccatctg gcgcggttgc ttccagaacg gcggctaccg
     7861 gtttgtcatc ctgtttggcg atgtaaaccc gatgctcacc tttacctaac tctggcgcag
     7921 ttaccagata gcaactctgt gcgagcgcat tgttatagcg ttcggctggc agcacctgat
     7981 caaataacgt cttttgttgc agactggcct gttcagcaat tgtcgttttg gtcatctggt
     8041 tgatggccgc agttaaccct gtagaacccg ctgcaaatag tgccagtgta atgccgtgtt
     8101 ttcggatagt tttcagcatg gtttaccctt tgcgatggcc gtagacgcgc ggacgcgtgt
     8161 agtaatcgat cagaggaacc gtgatgttcg ccagcaggac ggcaaaagcc acgccgtcag
     8221 gatagccgcc gaaactgcgg atcaaccaga ctaataagcc cgccagcgcg ccgaacatca
     8281 gccgaccacg attggtcgta gaagcagtaa ctgggtcagt caaaataaag aatgcgccga
     8341 gcatagtcgc accagacagc agatgaattt gcggtgctgc cagtgtttct ggtgagaaca
     8401 accagcccaa cgttgcgcat aacgccagcg ttactaagaa gctgagggga atatgccagc
     8461 gaatcgcttt ctgccatagc aaccacacgc caccagccag ccaggcgaga tttacccatt
     8521 gccaaccagc gcccgccaga ataccgctgt agatcggata ttgcataatc tgttcaaccg
     8581 aatgacctgc gcggacagag gttttgaatg tatccagcgg tgtcgcctga ctaatgccat
     8641 caatacctaa gcgtagcgta ttcatatcac caccactggc ggtatgaccg ctgaaaataa
     8701 cctgtatggc gtcgataaaa ccagggatgt tgaccgcaat ttcatgtggc ggtaaccagc
     8761 tggtcatctg caccgggaag gagatcagta agaccacata accaatcatt gccggattaa
     8821 acgggttttg ccccagaccg ccatacaact gtttagcgat gatcaccgca aacaccgtac
     8881 ccagtacgac catccaccat ggcgctaggg ggggaatact taccgccagc aataagcctg
     8941 tcagcaatgc tgagttatct ttcaacgttg cggctaccga ctgcttgcgt agtttgagta
     9001 cgagagcttc ggctaacaga gcactgaccg acgccaacag gatctgaacg agagtacccc
     9061 aaccaaaaaa ccgcagttgc gctgcgattc ctggcacggc tgcgagcaac accaacagca
     9121 taatgcgcga tgtctggcgc tggttatggg tataagggga gctagctatt ctgaatacca
     9181 tttagtcctc gtttacaacc ttttgctggg cggctttttt ggcctgaacg cgggcaatag
     9241 ccgcggcaac tgcggctttg cgcggatcga cctgttcttc tggttccgca ttagcctgtt
     9301 gctgttccag cttgcgtgct ttggcgcg
//
LOCUS       DAZPCS010000145         9184 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZPCS010000145 DAZPCS010000000
VERSION     DAZPCS010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9184
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.146"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..923
                     /locus_tag="ACHUQ7_003257"
     CDS             <1..923
                     /locus_tag="ACHUQ7_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1136543.1"
                     /translation="GIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQAIAPP
                     ALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSASVKPRPQ
                     LQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGWVAAIP
                     FLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSPAQAVA
                     FISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGWVVDTT
                     HSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            1175..2092
                     /gene="oxyR"
                     /locus_tag="ACHUQ7_003258"
     CDS             1175..2092
                     /gene="oxyR"
                     /locus_tag="ACHUQ7_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFR1136544.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVLMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPCRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(2075..3475)
                     /gene="sthA"
                     /locus_tag="ACHUQ7_003259"
     CDS             complement(2075..3475)
                     /gene="sthA"
                     /locus_tag="ACHUQ7_003259"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFR1136545.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSNSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            3796..4446
                     /gene="fabR"
                     /locus_tag="ACHUQ7_003260"
     CDS             3796..4446
                     /gene="fabR"
                     /locus_tag="ACHUQ7_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFR1136546.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            4446..4805
                     /locus_tag="ACHUQ7_003261"
     CDS             4446..4805
                     /locus_tag="ACHUQ7_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFR1136547.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4845..5945)
                     /gene="trmA"
                     /locus_tag="ACHUQ7_003262"
     CDS             complement(4845..5945)
                     /gene="trmA"
                     /locus_tag="ACHUQ7_003262"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFR1136548.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      6059..6249
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6314..8158
                     /gene="btuB"
                     /locus_tag="ACHUQ7_003263"
     CDS             6314..8158
                     /gene="btuB"
                     /locus_tag="ACHUQ7_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFR1136549.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKALNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            8103..8960
                     /gene="murI"
                     /locus_tag="ACHUQ7_003264"
     CDS             8103..8960
                     /gene="murI"
                     /locus_tag="ACHUQ7_003264"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFR1136550.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2102 a         2220 c         2481 g         2381 t
ORIGIN      
        1 tgggtattgg cgaagcgcca tttatgcctg cgggagtaaa gtcgatcacc gactggtatg
       61 cacaaaaaga acgcggcacg gcgctgggga tttttaactc atctaccgtt atcggtcagg
      121 ctatcgcgcc tcctgctctt gtattgatgc agctggcatg gggctggcgg acgatgttcg
      181 ttatcatcgg cgtggcaggg attctggttg ggatctgttg gtacgcgtgg tatcgcaacc
      241 gggcgcagtt tgtcctgact gacgaagaac ggacgtatct ctccgcgtcg gttaaaccgc
      301 gtccacagct gcaatttagc gagtggctgg cgctgtttaa gcatcggaca acctggggga
      361 tgattttagg tttctctggc gtcaattata ccggttggct ttacattgcg tggttacccg
      421 gttatttgca agccgaacaa ggtttcagtc tggcgaaaac cggttgggta gcggcgattc
      481 ctttcctcgc ggcggcggtc gggatgtggg ttaacggtat tgtggtcgat cggctggcga
      541 aaaaaggcta cgacctggcg aagacgcgta aaacggctat tgtctgcggt ttgatgatgt
      601 cggcattagg cacgttgctg gtcgtgcaat cttcctcgcc agctcaggcg gtagcgttta
      661 tctcaatggc gctgttctgc gtgcatttcg cgggaacgtc tgcatgggga ctggtgcagg
      721 tgatggtgtc agaaacaaaa gtggcttcca tcgccgggat tcaaaacttt ggcagttttg
      781 tttttgcttc ctttgctccg atcgtaaccg gttgggtagt ggataccaca cactcgttta
      841 atctggcgct ggttatcgcg gcctgtgtga cgttcaccgg ggcgctgtgt tacttcttta
      901 ttgtcaaaga tcgcattgag taatcgttac tatcaggctc tcttgctgtg ggcctgtaga
      961 ataaaaaaaa gcggatcctg gagatccgca aaagttcacg ttggctttag ttattcgagt
     1021 tgagaaactc tcgaaacggg cagtgacttc aagggttaaa agaggtgccg ctccgtttct
     1081 gtgagcaatt atcagtcaga atgcttgata gggataatcg ttcattgcta ttctacctat
     1141 cgccatgaac tatcgtggcg atggaggatg gataatgaat attcgtgatc ttgagtacct
     1201 ggtggcattg gctgaacacc gccattttcg gcgtgcggca gattcctgcc acgttagcca
     1261 gccgacgctt agcggccaaa ttcgtaagct ggaagatgag ctgggcgtga tgttgctgga
     1321 gcggaccagc cgtaaagtgt tgttcaccca ggcgggaatg ctgctggtgg atcaggcgcg
     1381 taccgtgctg cgtgaggtga aagtccttaa agagatggca agccagcagg gtgagacgat
     1441 gtccggaccg ctgcatattg gtttgattcc tacggttgga ccatacctgc taccgcatat
     1501 tatcccgatg ctgcaccaga catttccaaa gctggaaatg tatctgcatg aagcacagac
     1561 ccaccagtta ctggcgcaac tggacagcgg caaactcgat tgcgtgatcc tcgcgctggt
     1621 gaaagagagc gaagcattca ttgaagtgcc gttgtttgat gagccaatgt tgctggctat
     1681 ctatgaagat cacccgtggg cgaaccgcga atgcgtactg atggccgatc tggcagggga
     1741 aaaactgctg atgctggaag atggtcactg tttgcgcgat caggcaatgg gtttctgctt
     1801 tgaagccggg gcggatgaag atacacactt ccgcgcgacc agcctggaaa ctctgcgcaa
     1861 catggtggcg gcaggtagcg ggatcacttt attgccagcg ctggctgtgc cgccggagcg
     1921 caaacgcgat ggggttgttt atctgccatg cattaagccg gaaccatgcc gcactattgg
     1981 cctggtttat cgtcctggct caccgctgcg cagccgctat gagcagctgg cagaggccat
     2041 ccgcgcaaga atggatggcc atttcgataa agtgttaaaa caggcggttt aaaccgttta
     2101 acgcagctac ccgataggct tccgccatcg tcgggtagtt aaaggtggtg ttgacgaagt
     2161 actcaatagt gttgccgcca cctttctgtt ccataatcgc ctgaccgata tgaataattt
     2221 cggcagcgcg ctcgccaaag cagtgaatac ccagaatctc ttttgtttcc cgatggaaca
     2281 aaattttcag cgtgcccacg ttcatgccga cgatttgtgc acgtgccaga tgtttgaact
     2341 gggcgcggcc cacttcatac ggcactttca tcgcggtcag ctgctgttcg gttttgccca
     2401 cagagctgat ttccgggatg gtgtaaatgc cggtagggat atcttcaatc agatgtgcgg
     2461 tggcttcgcc ttttaccagc gcctgcgcgg caatgcgccc ctggtcatag gccgctgatg
     2521 ccaggctcgg ataaccaatc acgtcgccca ccgcgtaaac gtgcggctgt gcggtctgat
     2581 acatgctgtt gaccttcagc tgtccgcgac tgtcagtttc cagcccaatg ttctgtaacg
     2641 ccagcgaatc ggtattaccg gtacgaccgt tggcatagag caggcagtca gctttcagtt
     2701 ttttacccga cttcagatgc atgatcacac cgtcgtcaca gctttcgatc ttctcgtact
     2761 cttcgttgtg acgaatcact acgccactgt tccagaagtg gtaggagaga gaatttgaca
     2821 tctcttgatc gagaaatgcc agcaggcgat cgcgggtgtt gatcagatcc acttttacat
     2881 ccataccgcg gaagatcgac gcatattcac agccgatcac tccagcacca tagataagta
     2941 catggcgcgg ttcgtggtgc atgctaagaa ttgagtcgct gtcgtaaatg cgtggatggg
     3001 tgaaatcaac atctgttgga tgatatggac gagagccgca ggcaataaca aatttttcag
     3061 cggttagtgt ttcaacgctg ccgtccgggc aatccagcgc caacgtatgc tcgtcaacaa
     3121 agcgagcgtt cccctgcaat atttcacagt gattacgttc gtaaaatccc tgacgcatgc
     3181 gcgtttgttg attaatcacg ttatcggcat ggttaaggat atcggcaaaa gaagagcgga
     3241 gcagtcggga atggtcgctg tatagtgggt tttgattgaa ttctataatg cggctgacgg
     3301 cgtgacggag agctttcgac gggatggtgc cccagtgggt gcaaccgccg ccaacatttt
     3361 gataacgctc gataactgcg acgcgcgcac cttgcttaac caggcccatt gcagcgcctt
     3421 cgccgccggg gccggaacct attactatgg catcgtaatc gtaggaatgt ggcatggtag
     3481 ggcttacctg ttcttataca taaaagcaac agaatggtaa cattctatcg cgggtaagcc
     3541 aattgatccc cgtcatttat ctggctatat cctgagcggc ctttgctttg tctgtttctt
     3601 acttttgccc tgacgtttta ttggattttt atcgacgata ctctccgttt aagcggcagg
     3661 tttccgctgt acgtaaaaga accggccagt aaatatgttt tattgcgtta ccgttcattc
     3721 acaatactgg agcaatccag tatgttcatt ctctggtata gtgccagcag tacttttggc
     3781 aaggattcag acatcgtgat gggcgtaaga gcgcaacaaa aagaaaaaac ccgccgttcg
     3841 ctggtggaag ccgcatttag ccaattaagt gctgaacgca gcttcgccag cctgagtttg
     3901 cgtgaagtgg cgcgtgaagc gggcattgct cccacctctt tttatcggca tttccgcgac
     3961 gtagacgaac tgggtctgac catggttgat gagagcggtt taatgctacg ccaactcatg
     4021 cgccaggcgc gtcagcgtat cgccaaaggc gggagtgtga tccgtacctc ggtctccaca
     4081 tttatggagt tcatcggtaa taatcctaac gccttccggt tattattgcg ggaacgctcc
     4141 ggcacctccg ctgcgtttcg tgccgccgtt gcgcgtgaaa ttcagcactt cattgcggaa
     4201 cttgcggact atctggaact cgaaaaccat atgccgcgtg cgtttactga agcgcaagcc
     4261 gaagcaatgg tgacaattgt cttcagtgcg ggtgccgagg cgttggacgt cggcgtcgaa
     4321 caacgtcggc aattagaaga gcgactggta ctgcaactgc gaatgatttc gaaaggggct
     4381 tattactggt atcgccgtga acaagagaaa accgcaatta ttccgggaaa tgtgaaggac
     4441 gagtaatgaa acaagcaaat caagatagag gtacgctgct gctggcgttg gttgctggtt
     4501 tatcgattaa tggtactttc gcagcgctgt ttagctccat tgtgccattt tctgtattcc
     4561 cgattatttc cctggtgctg acggtttact gcctgcatca acgttatctt aatcgcacca
     4621 tgtcggtagg cttgccgggt ctggcagctg cctgttttat tctcggcgta ctgctgtaca
     4681 gcacggtagt tcgtgcggaa tatccggata tcggctctaa cttcttcccg gcagtactct
     4741 ccgtcattat ggtgttctgg attggcgcga agatgcgtaa ccgtaagcag gaagttgctg
     4801 agtaatcggt atcatgccgg atgctggcgc atccggcatg ggttttactt cgcggtcagt
     4861 aatacgccgc actccatatg gtgcgtgtag gggaactgat caaacagagc cagacgttcg
     4921 accttgtgcg tctggcttaa tgtttccaga tttttgcata acgtttccgg attgcaggag
     4981 atatacagaa tacgtggata cgcctgcacc attttctcgg tttcactgtc cagaccgctg
     5041 cgcggtgggt cgacaaaaat ggtttcgaac tgatagctct ttaagtcgat cccttgcagg
     5101 cggttaaact cgcgcacacc attcatcgcc tgagtaaatt cttctgccgc catacgaata
     5161 atttgtacgt tatcaatatg gttagcggca atgttgtatt gcgcggcagc gactgacggt
     5221 ttggcgattt cggtggctaa tacccgatca aaattgcgcg ccagcgctaa tgaaaagtta
     5281 ccgttgccgc agtacagctc cagtaaatcg ccttttgagc ctttggttac gtccagcgcc
     5341 cattccagca tctgaatatt catcgccgcg ttcggctggg taaagctgtt ttctacctga
     5401 cgatagatca tctctttccc tgcgaccggc agacgctcgt cgatgtaatc ttgatccagc
     5461 gcgattttgg ttttcgttgc ccgaccaatc agatgcacat tcagattctg cgcgcgcagt
     5521 gcatcgcgca gtgcttccgc ttcctgacgc cactcatcat ccagcttttt atggtacagc
     5581 agggaaacca ccgcctgatt actcagcgtg gtgaggtaat caatctggaa caacttgtgg
     5641 cgcagaacgg gattattacg cacacccgca atcatcgccg tcatcaactg gttgatgagt
     5701 tcactggcgg cggggaagct atccacgcgg atgcggcttt tggtttgttg atcgaaaatg
     5761 atgtgataca ggtcatcgcc atcgtgccag atgcggaact ccgcgcgcat ccggtaatga
     5821 ctgaccggcg agcgaaacac ttccggaacc aggtcagaaa acggtgccat catactttgc
     5881 aaacgtacca ctttttcggc taactgcgct tcatactgtt ctgttggaag gtgttcgggg
     5941 gtcatgatgt atcctgaaaa attaaagtac gcggcgattg tagggattgc tcatcagatg
     6001 tccagatctt gatgaattcc tatttgtgag ctacgtctgg acagtaactt gttacaacct
     6061 gtagcatcca cttgccggtc ctgtgagtta atagggaatc cagtgcgaat ctagagctga
     6121 cgcgcagcgg taaggaaagg tgcgatgatt gcgttatgcg gacactgcca tccggtggga
     6181 agtcatcatc tcttagtatc ttagataccc ctccaagccc gaagacctgc cggccaacgt
     6241 cgcatctggt tctcatcatc gcgtaatatt gatgaaacct gcggcatcct tcttctattg
     6301 tggatgcttt acaatgatta aaaaagcttc gctgctgacg gcgtgttccg tcacggcatt
     6361 ttccgcttgg gcacaggata ccagcccgga tactctcgtc gttactgcta tccgttttga
     6421 acagccgcgc agcactgtgc ttgcaccaac caccgttgtg acccgtcagg atatcgaccg
     6481 ctggcagtcg acctcggtca atgatgtgct gcgccgtctt ccgggcgtcg atatcaccca
     6541 aaacggcggt tcaggtcagc tctcatctat ttttattcgc ggtacaaatg ccagtcatgt
     6601 gctggtgtta attgatggcg tccgcctgaa tctggcgggg gtgagtggtt ctgccgacct
     6661 tagccagttc cctattgcgc ttgtccagcg tattgaatat atccgtggac cgcgctccgc
     6721 tgtttatggt tccgatgcaa taggcggggt ggtgaatatc atcacgacgc gcgatgaacc
     6781 cggaacggaa atttcagcag ggtggggaag caatagttat cagaactatg atgtctctac
     6841 gcagcaacaa ctgggggata aaacacgggt aacgctgttg ggcgattatg cccatactca
     6901 tggttatgat gttgttgcct atggtaatac cggaacgcaa gcgcagacag ataacgatgg
     6961 ttttttaagt aaaacgcttt atggggcgct ggagcataac tttactgatg cctggagcgg
     7021 ctttgttcgc ggctatggct atgataaccg taccaattat gacgcgtatt attctcccgg
     7081 ttcaccgttg ctcgataccc gtaaactcta tagccaaagt tgggacgccg ggctgcgcta
     7141 taacggcgaa ctgattaaat cacaactcat taccagctat agccatagca aagattacaa
     7201 ctacgatccc cattatggtc gttatgattc gtcggcgacg ctcgatgaga tgaagcaata
     7261 caccgtccag tgggcaaaca atgtcatcgt tggtcacggt agtattggtg cgggtgtcga
     7321 ctggcagaaa cagactacga cgccgggtac aggttatgtt gaggatggat atgatcaacg
     7381 taataccggc atctatctga ccgggctgca acaagtcggc gattttacct ttgaaggcgc
     7441 agcacgcagc gacgataact cacagtttgg tcgtcatgga acctggcaaa ccagcgccgg
     7501 ttgggaattc atcgaaggtt atcgcttcat tgcttcctac gggacatctt ataaggcact
     7561 aaatctgggg caactgtatg gcttctacgg aaatccgaat ctggacccgg agaaaagcaa
     7621 acagtgggaa ggcgcgtttg aaggcttaac cgctggggtg aactggcgta tttccggata
     7681 tcgtaacgat gtcagtgact tgatcgatta tgatgatcac accctgaaat attacaacga
     7741 agggaaagcg cggattaagg gtgtcgaggc gacagccaat tttgataccg gaccactgac
     7801 gcatactgtg agttatgatt atgtcgatgc gcgcaatgcg attaccgaca cgccgttgtt
     7861 acgccgtgct aaacagcagg tgaaatacca gctcgactgg cagttgtatg acttcgactg
     7921 gggtattact tatcagtatt taggcactcg ctatgataag gattactcat cttatcctta
     7981 tcaaaccgtt aaaatgggcg gtgtgagctt gtgggatctt gcggttgcgt atccggtcac
     8041 ctctcacctg acagttcgtg gtaaaatagc caacctgttc gacaaagatt atgagacagt
     8101 ctatggctac caaactgcag gacgggaata caccttgtct ggcagctaca ccttctgaac
     8161 cacgtcccac cgtgctggtg tttgactccg gcgtcggtgg gttgtcggtc tatgacgaga
     8221 tccggcatct cttaccggat ctccattaca tttatgcttt cgataacgtc gctttcccgt
     8281 atggcgaaaa aagcgaagcg tttattgttg agcgagtggt ggcaattgtc accgcggtgc
     8341 aagaacgtta tccccttgcg ctggctgtgg tcgcttgcaa caccgccagt accgtttcac
     8401 ttcctgcatt acgcgaaaag ttcgacttcc cggttgttgg tgtcgtgccg gcgattaaac
     8461 ctgctgcacg tctgacggca aatggcattg tcggattact ggcaacccgc ggaacagtta
     8521 aacgttctta tactcatgag ctgatcgcgc gtttcgctaa tgaatgccag atagaaatgc
     8581 tgggctcggc agagatggtt gagttggctg aagcgaagct gcatggcgaa gatgtttctc
     8641 tggatgcact aaaacgtatc ctacgcccgt ggttaagaat gaaagagccg ccagataccg
     8701 ttgtattggg ttgcacccat ttccctctac tacaagaaga actgttacaa gtgctgccag
     8761 agggaacccg gctggtggat tctggcgcag cgattgctcg ccgaacggcc tggttgttag
     8821 aacatgaagc cccggatgca aaatctgccg atgcgaatat tgccttttgt atggcaatga
     8881 cgccagaagc tgaacaatta ttgcccgttt tacagcgtta cggcttcgaa acgctcgaaa
     8941 aactggcagt tttaggctga tttggttgaa tgttgcgcgg tcggaaaatt attttaaatt
     9001 tcctcttgtc aggccggaat aactccctat aatgcgccac cactgacacg gaacaacggc
     9061 aaacacgccg acgggtcagc ggggttctcc tgagaattcc ggcagagaaa gcaaaaataa
     9121 atgcttgact ctgtagcggg aaagcgtatt atgtacaccc cgcgcagctg agaaaaagcg
     9181 aagc
//
LOCUS       DAZPCS010000146         9029 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZPCS010000146 DAZPCS010000000
VERSION     DAZPCS010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9029
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.147"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(97..1473)
                     /gene="trkA"
                     /locus_tag="ACHUQ7_003265"
     CDS             complement(97..1473)
                     /gene="trkA"
                     /locus_tag="ACHUQ7_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFR1136551.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="ACHUQ7_003266"
     CDS             complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="ACHUQ7_003266"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFR1136552.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIAEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRIYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="ACHUQ7_003267"
     CDS             complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="ACHUQ7_003267"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFR1136553.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINIHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3792..4301)
                     /gene="def"
                     /locus_tag="ACHUQ7_003268"
     CDS             complement(3792..4301)
                     /gene="def"
                     /locus_tag="ACHUQ7_003268"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFR1136554.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVQ
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4466..5590
                     /gene="dprA"
                     /locus_tag="ACHUQ7_003269"
     CDS             4466..5590
                     /gene="dprA"
                     /locus_tag="ACHUQ7_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFR1136555.1"
                     /translation="MVDIEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5562..6035
                     /gene="smg"
                     /locus_tag="ACHUQ7_003270"
     CDS             5562..6035
                     /gene="smg"
                     /locus_tag="ACHUQ7_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFR1136556.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            6064..6606
                     /locus_tag="ACHUQ7_003271"
     CDS             6064..6606
                     /locus_tag="ACHUQ7_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFR1136557.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6611..7183
                     /gene="tsaC"
                     /locus_tag="ACHUQ7_003272"
     CDS             6611..7183
                     /gene="tsaC"
                     /locus_tag="ACHUQ7_003272"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFR1136558.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            7188..8006
                     /gene="aroE"
                     /locus_tag="ACHUQ7_003273"
     CDS             7188..8006
                     /gene="aroE"
                     /locus_tag="ACHUQ7_003273"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFR1136559.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            8003..8260
                     /locus_tag="ACHUQ7_003274"
     CDS             8003..8260
                     /locus_tag="ACHUQ7_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFR1136560.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8236..8790)
                     /locus_tag="ACHUQ7_003275"
     CDS             complement(8236..8790)
                     /locus_tag="ACHUQ7_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFR1136561.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         2259 a         2191 c         2251 g         2328 t
ORIGIN      
        1 ctaacaaatg ttaagccatt ttcctgccag gaatgaattt aattaatcct tgataatcaa
       61 agggaaataa taaggcgtca ttagacgcct tattaattac aagaagaaag ggcttggctg
      121 gaagagtctt tcgacgtcgg taataaactt tttatctgtg aggaacataa ttacgtgatc
      181 gccttgctca atgcgcagat tgtcattggc aatcatcacg tcgtttccac gtaccaccgc
      241 tccaataatc gttcctggcg gtagcttgat ttcgtcaatg actctgccga caacgcgtga
      301 ggtgctttca tcaccgtgag caacggcttc aatagcttct gctacgccgc ggcgcaatga
      361 ggaaacacca acaatatctg cttttcgcac atggctaagc aacgcagaaa tagttgcttg
      421 ttgtggtgaa attgcaatat cgatcacgct cccctgaacc agatccacat aagcgcgacg
      481 ctggatcaat accatcacct ttttcgcacc catacgtttg gcaagcatgg cggacatgat
      541 attggcctcg tcatcgttgg tgacggcaat aaacagatca acttgatcga tatgttcttc
      601 ggccagcagt tcttgatccg acgcatcacc aaaaaagacg atcgtattct gcaacttttc
      661 cgccagttcg gcagcgcgct gctgattacg ttcgatgagt ttaacgctgt aatctttttc
      721 cagacgacgc gccagccctg caccgatatt accgccacca accagcatga tccgtttata
      781 cggtttttcc agtcgctgta attcactcat caccgcgcgg atatgctgtg aagcggcaat
      841 aaagaacact tcatcaccag cttcaacaat ggtcgaacct tgtggacgaa tggggcgatc
      901 gtggcggaaa attgctgcca cacgagtatc gatatgtggc atatgttcgc gcatggtcga
      961 aagtgcatta ccaatcagcg ggccgccata ataggcttta actacagcga ggctgacttt
     1021 accctcagcg aagttcacca cctgcaatgc gcctggatac tcaatcagtc ggtaaatatt
     1081 atcgataacc aactgctctg gtgcgatcag atgatcaatc ggtacagcat ctgaatgaaa
     1141 tagcttatcg gcatcgcgta cgtagtctgg tgagcggatg cgagcgatgc gattaggggt
     1201 gttgaaaagt gagtaggcta cctggcaggc aaccatattg gtttcatctg aactggttac
     1261 agcaaccagc atatcggcgt cgtcggcacc tgcctcccgc aatacgcgtg gatgagagcc
     1321 atgcccctgc acgactcgca ggtcaaattt atcctgcaag gtccgcagac gctcaccgtt
     1381 ggtatcgaca acagtaatat cgttgttctc gccaaccagg ttttccgcca gtgtgccgcc
     1441 aacctggccg gcacccagaa tgataatttt catcagtcgc gacccgttat ctcatcactt
     1501 tttgattagc ttagcgtaaa agaagccgtc gccctcttcg gcaccaggct gattttgttt
     1561 acccggttgc tctggtgttc ctgtttcgca aagttcggca tcagcggtac gttgcaaaaa
     1621 ggctttaatc tgcaggctat tctcttccgg taacaccgaa caggtggcat agaccagggt
     1681 tccaccggat tttaaatgcg gccaaatggc gtcgagaatt tcagactgca attgcgcgag
     1741 ttccgggata tcgcgatcgc gacgtaacca tttaatatct ggatggcgac gaatcacccc
     1801 cgttgctgaa caaggcgcat ctaataaaat gcgatcaaac tgttgctcgc cacaccattg
     1861 ggaagggtaa cggccatcac cttgtttcac ggttgccttc ataccaaggc gttttaaatt
     1921 gtcgtaaatg cgagagaggc gctgttcgtc aatatcaacc gcaacaacct gcgcttctgg
     1981 tgccacctca aggatatgcg ttgttttacc gccgggggcg gcacaaagat ccaagatgtg
     2041 ttcaccgttt tgtggcgcaa gccaggtcat gcaaccttgt gctgatgcat cctgaacggt
     2101 aacccatccg tcttcaaaac caggtagcgc atgaacaggt gcaggtgttt ccagccgtac
     2161 agcatcaggg taatccgcat gtgggaaacc tttcattcct gcttcatcca gcaatgcaag
     2221 ccagctgtcg cgggaatgat gcgtacgatt aatacgcagc cacattggcg gacgctggtt
     2281 attggcttcg gcgatggatt gccactgctc tggatacgct ttttgcagac gcttcagcaa
     2341 ccaggaagga tgcagataac gtgcatcacg ggtattaaac tcggctaata actcttcttg
     2401 ccgacgctgg aactggcgta atacaccgtt aatcaaccct ttaagttgcg gacgcttaat
     2461 tgcgatagcg ccttcaaccg tttcagccag cgcagcatga ggtggaatgc gggtataaag
     2521 cagttgatac aaaccaacca taatcaggta atgcacagtg cgctgtttgc cggtcatcgg
     2581 acgggccatt aacttattaa tcagccagtc taactgcgaa agcgtacgca gtacgccaaa
     2641 gcacaactct tgaagaagtg ctttgtcttt atcggaaact ttttgctgga gcggtggcag
     2701 aatgttgctt aatgattgcc cttgctcgac gacttgttca acggcctggg ccgccatgct
     2761 acgtaaatta cgttgttttt tcataagtat aaaaatgctc ggcaagaccg ggcttagaag
     2821 agtggactat cagaccagac ggttgcccgg aacaaaccat tcccgacgag agttcaggag
     2881 gtcttgcgcg ctcatcgctt tcttacccgc aggttgtaac gagagcaggt tcaggatgcc
     2941 atcaccagtc gcaacctgaa tgccttgttt gttggcttca aggatcgttc ctggtgcagc
     3001 gttggttgcc gtatcaatga ccgacgcttt ccagacttta accggctgtc cttcaatttc
     3061 cagccagctc attggccatg ggttgaaagc gcgaatgcag cgttcaagct gtgctgccga
     3121 aagtgaccag tcaatacgcg cttcttcttt actcaacttc tcggcgtaag tgacaagagt
     3181 ttcgtcctga acttctggtt tcgccgtgcc gtctgccaat tgtttcaacg tggtgataag
     3241 cccttgtggg ccaagctctg ccagcttgtc gtacaacgta ccactggtat cttctgcagt
     3301 aatcgggcag gagagcttat agagcatgtc accggtgtct aaaccaacat ccatttgcat
     3361 aatggttaca ccagtttctg catcacccgc ccatagtgag cgttggattg gtgcagcacc
     3421 gcgccagcgt ggcagcagtg aaccatgaat gttgatacag ccaagacgcg gcatctccag
     3481 cacagcttta ggcagaatta aaccataggc gacgacgacc ataacatcag cctgcaaatc
     3541 ggcgaccagt tgctggtttt cttgtggacg cagggaaaca ggttgaaaaa cgggcagacc
     3601 ttcttcctca gccagaactt taatcgggct gggcatcagt tttttaccgc gtcctgccgg
     3661 tcggtctggc tgggtgaaca cgccaacgac gttatgacca gaagacaaca gcgcgtcgag
     3721 atgacgcgct gcaaagtcag gtgtacccgc aaaaataata cgtagtgatt ctgacacgtt
     3781 agttcttatc cttaagcccg ggctttcaga cgatccagtt tttcaacttt ctgacgaatt
     3841 cgttgttgtt tcagcggtga cagataatcc ataaacagtt tgccaaccag gtgatccatc
     3901 tcatgctgaa tacagatagc taacagaccc tctgcttcca gttcaaatgg tttaccgtcg
     3961 cggtcaaggg cgcgaatttt aactttctct gcgcgctgca ctaaagcacg ttgttcaggg
     4021 atcgacaggc aaccttcttc aatgcctgtt tcgccgcttt tttctaaaag ctccggattg
     4081 attaacacca gccgttcgtc acggttttcc gaaacatcaa taacaatgat acgttgatgg
     4141 atatcaacct gggttgccgc caggccaata ccttcttctg cgtacatcgt ctcgaacata
     4201 tcatcgacga tacgctgaat ttctgcattc acttcttcta ccggtttagc aactttgcga
     4261 agccgctcgt ccggaatatg taacacttgc aaaactgaca taaatctcca gagatgtgtt
     4321 caggagttag aaagattatt tcttctattc tagacaaatc cccctctgat tgacagcatc
     4381 acttgattga cagcatcact tgattgacag catcactgac caatcgcaaa gattgctaag
     4441 gcttgcttat ggcagggaga taaggatggt cgatatagaa atttggctgc gtttaatgag
     4501 catcagcagc ttgtacggcg atgatatggt ccgtatcgct cactggctgg caaaacagtc
     4561 gcatattgat gcggttgtat tgcagcaaac agggcttaca ttgcggcagg cacaacgctt
     4621 tctttcattt ccacgaaaga gtatcgaaag ctcactttgt tggttggagc aacccaacca
     4681 tcatttaatc cctgcggaca gcgaatttta tcctcctcaa cttcaggcga cgacagatta
     4741 ccccggcgca ctgtttgttg aaggagaact gcacgcgctg cattcatttc agcttgccgt
     4801 agtggggagt cgggcgcatt catggtatgg cgagcgatgg gggcgattat tttgcgaaac
     4861 tctggcgacg tgtggagtga caattacgag tggactggcg cgtggaatcg atggtgtggt
     4921 gcataaagcg gccttacagg taaatggcgt cagcattgct gtattgggga atggacttaa
     4981 taccattcat ccgcgtcgcc atgcccgact ggctgccagt ctgtttgaac agggtggcgc
     5041 tctcgtctcg gaatttcccc tcgatgttcc accccttgct tacaatttcc cacgaagaaa
     5101 tcgcattatc agtggtctaa gtaaaggtgt actggtggtg gaagcggctt tgcgcagtgg
     5161 ttcgctggtg acagcacgtt gtgcgcttga gcaggggcgt gaagtttttg ccttgccagg
     5221 tccaataggg aatccgggaa gcgaagggcc tcactggtta ataaaacaag gtgcgattct
     5281 tgtgacggaa ccggaagaaa ttctggaaaa cttgcaattt ggattgcact ggttgccaga
     5341 cgcccctgaa aattcatttt attcaccaga tcagcaagac gtggcattgc catttcctga
     5401 gctcctggct aacgtaggag atgaggtaac acctgttgac gtcgtcgctg aacgtgccgg
     5461 ccaacctgtg ccagaggtag ttactcaact actcgaactg gagttagcag gatggatcgc
     5521 agctgtaccc ggcggctatg tccgattgag gagggcatgc catgttcgac gtactaatgt
     5581 atttgtttga aacctatatt cacactgaag ctgagttgcg tgtggatcaa gacaaacttg
     5641 aacaggatct taccgacgca ggttttgagc gagaagatat ctacaatgcc ctgctatggc
     5701 tggaaaaact tgctgattat caggaagggt tggcagaacc gatgcaactg gcctcggatc
     5761 ctctctccat gcgtatttat acaccggaag agtgtgaacg actggatgcc agctgccgtg
     5821 ggtttctgct tttccttgag cagattcagg tgctcaacct tgaaactcgt gaaatggtga
     5881 tagagcgagt gctcgcgctg gataacgcag agttcgaact ggatgatctg aaatgggtga
     5941 tcctgatggt gttgttcaac atcccgggct gcgaaaatgc gtaccagcaa atggaagaat
     6001 tactctttga agtgaatgaa ggtatgctgc attaatcatt tctttaattc agcataagtt
     6061 gttatggcaa aatcagcact gttcacggtg cgtaataatg agtcctgccc aaagtgcggg
     6121 gctgaactgg ttattcgatc cgggaaacac ggtccgtttc ttggatgctc acagtatccg
     6181 gcgtgtgact acgtccgtcc tctgaaatct tcagcggatg gacatattgt caaagttctg
     6241 gaggggcagg tttgccccgt atgtggcgca aatctggtat tacgccaggg acgctttggt
     6301 atgtttattg gttgcagtaa ctaccctgaa tgcgaacata ccgaacttat cgataaaccg
     6361 gacgaaacag caattacatg cccccaatgt cggacgggcc atctggtcca gcgccgctcc
     6421 cgttatggca aaacatttca ctcctgtgat cgctacccgg agtgtcaatt tgccattaac
     6481 ttcaaaccaa tagctggaga atgccctgag tgtcattatc cgctactcat cgaaaagaaa
     6541 accgcgcagg gtgtaaaaca cttttgtgcc agtaaacaat gtggaaagcc ggtttcggcg
     6601 gaataataac gtgaataata acctgcaagg agacgctatc gcagctgcga tagatgtcct
     6661 caatgaaaaa cgtgtcatcg cctatccaac ggaagccgtt ttcggtgtcg ggtgcgatcc
     6721 tgatagcgaa acagcagtga tgagactgtt ggagttaaaa cagcgtccgg ttgataaggg
     6781 gctgatttta atcgcggcaa attacgagca gcttaaaccc tatattgatg acaccatgtt
     6841 gactgacgtg cagcgtgaaa ccattttttc ctgctggcca ggtcctgtca cctttgtctt
     6901 tcccgcgcct gcgacaacac cgcgctggtt gacgggccgc tttgattcgc ttgctgtacg
     6961 agtcaccgac catccgttgg tggttgcttt gtgccaggct tatggtaaac cgctggtttc
     7021 taccagtgcc aacttgagtg gattgccacc ttgtcgaaca gtagacgaag ttcgcgcaca
     7081 atttggcgcg gcgttcccgg ttgtgcctgg tgaaacgggg gggcgtttaa atccttcaga
     7141 aatccgcgat gccctgacgg gtgaactgtt tcgacagggg taacataatg gaaacctatg
     7201 ctgtttttgg taatccgata gcccacagca aatcgccatt cattcatcag caatttgctc
     7261 agcaactgaa tattgaacat ccctatgggc gcgtgttggc acccatcaat gatttcatca
     7321 acacactgaa cgctttcttt cgtgctggtg gtaaaggtgc gaatgtgacg gtgcctttta
     7381 aagaagaggc ttttgccaga gcggatgagc ttactgaacg ggcagcgttg gctggtgcag
     7441 ttaataccct caagcggtta gaagatggac gcctgctggg tgacaatacc gatggtgtag
     7501 gcttgttaag cgatctggaa cgtctgtctt ttatccgccc tggtttacgt attctgctta
     7561 tcggcgctgg tggagcatct cgcggcgtac tactgccact tctttccctg gactgtgcgg
     7621 tgacaatcac taatcggacg gtatcccgcg cggaagagtt ggctaaattg tttgcgcaca
     7681 ctggcagtat tcaggcgttg ggtatggacg aactggaagg tcatgagttt gatctcatta
     7741 ttaatgcaac atccagtggc atcagtggtg atattccggc gatcccgtca tcgctcattc
     7801 atccaggcat ttattgctat gacatgttct atcagaaagg aaaaactcct tttctggcat
     7861 ggtgtgagca gcgaggctca aagcgtaatg ctgatggttt aggaatgctg gtggcacagg
     7921 cggctcatgc ctttcttctc tggcacggtg ttctgcctga cgtagaacca gttataaagc
     7981 aattgcagga ggaattgtcc gcgtgaatca ggccatccag tttccggaca gggaagagtg
     8041 ggacgagaat aaaaaatgtg tatgttttcc cgctctcgtg aatggtatgc aactgacatg
     8101 cgcgatctct ggcgagagtc tggcgtatcg ctttactgga gatacgccag aacagtggtt
     8161 agcgagtttt cgtcagcatc gctgggacct ggaagaagaa gcggaaaact taattcagga
     8221 acaaagtgaa gatgatcaag gctgggtctg gttaccctga tccagatatt cgtccttcca
     8281 tttcacgtaa ttattcgcgg aatagcgtaa cccagccttc tcttcatcac ttaacgggcg
     8341 gatctgtttg acggggctac cgagatacag atatccgctc tccagccgtt tattttgtgg
     8401 gaccagacta cccgcaccaa tcatcacatc atcttctact attgcgccat caagtaaaat
     8461 tgagcccatc ccaaccaaaa ctcgattgcc aatggtgcag ccgtggagca tcaccttgtg
     8521 accaacagtg acatcttcgc caatggttaa tgggttgcca tctgggttgt acgaggattt
     8581 atgagtgaca tgcaacatac tgccatcctg gatattggtg cgtgctccga tctgtacata
     8641 atgtacatct ccacgaatca caacgagcgg ccagatcccc acatcatcag ccagacgaac
     8701 gtcaccaatc acgacactgc tatcgtcgat cattacgcgc tgaccgattt gtggaaaaag
     8761 atcgcggtat gggcgtaaaa catcagacat acttacctca gcaataaatg atttactaat
     8821 gactttgggg gcattattgg ccttgtgcaa gtcttttagt atgcaaaaaa gcaccgtttt
     8881 gtgtgcgatt gcagcaaaaa gggtgaaaaa acaacaaaca gaaaagaaga tcaaaaaaat
     8941 acttgtgcaa aatattggga tccctataat gcgcctccgt tgagacgaca acgtgaaaca
     9001 cttcacagga tggtcggaac aacgaagag
//
LOCUS       DAZPCS010000147         8778 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZPCS010000147 DAZPCS010000000
VERSION     DAZPCS010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8778
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.148"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            82..390
                     /locus_tag="ACHUQ7_003276"
                     /pseudo
     CDS             82..390
                     /locus_tag="ACHUQ7_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
     gene            362..2289
                     /locus_tag="ACHUQ7_003277"
                     /pseudo
     CDS             362..2289
                     /locus_tag="ACHUQ7_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
     gene            2273..2476
                     /locus_tag="ACHUQ7_003278"
     CDS             2273..2476
                     /locus_tag="ACHUQ7_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HFR1136562.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLNELN
                     RAINDAESVLGTTRRRRPLGVRL"
     gene            2473..4066
                     /locus_tag="ACHUQ7_003279"
                     /pseudo
     CDS             2473..4066
                     /locus_tag="ACHUQ7_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            4056..5561
                     /locus_tag="ACHUQ7_003280"
     CDS             4056..5561
                     /locus_tag="ACHUQ7_003280"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFR1136563.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADTQVRGVLLDIDSSGGQATGAFDCADMIYRLRQLKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMGHVSYAGHLAQAGVDITLIYAGSHKVDGN
                     QFESLPAKVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVMGTEAAVFEGQSGIEAELA
                     DELINASDAISVMAATLNTHDTGGTMPQLTATDAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAVAAPQQSGVSAQSEADRIMACEEAKGREQLAATLAAMPE
                     MTVEKARPILAASPQANAGPSLRDQIMALDEAKGAEAQAEKLAACPGMTVENARAVLA
                     AASGRAEPVSASTTAMFEHFMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            5598..5945
                     /locus_tag="ACHUQ7_003281"
     CDS             5598..5945
                     /locus_tag="ACHUQ7_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFR1136564.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGCSGISSATPALTPLMLD
                     EDTGKLVVWDGQKAGHVVGILVLPLEGTETVLTYYKSGTFATEAIHWPERVDTHKKAN
                     AFAGSALSHAALP"
     gene            6003..7031
                     /locus_tag="ACHUQ7_003282"
     CDS             6003..7031
                     /locus_tag="ACHUQ7_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFR1136565.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     GPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGEQFETVEVDFGRSVGN
                     NITQAGGTEWSAQDRDTFDPTHDLDAYCDFASGTINIAIMDGKVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPDGTLVLG
                     NTAAEGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            7083..7469
                     /gene="gpFI"
                     /locus_tag="ACHUQ7_003283"
     CDS             7083..7469
                     /gene="gpFI"
                     /locus_tag="ACHUQ7_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFR1136566.1"
                     /translation="MATKEENLKRLCELAERLGREPDVSGSAADIAQRVAELEEELGD
                     AGEVAEPDTYSPSKDDPTAHKGESVPEQPGIVTGDETGQVTVVALATLHTKCNGSVIF
                     VSPGTSFRVSAEVAASMAAGGLAKRQ"
     gene            7481..7858
                     /locus_tag="ACHUQ7_003284"
     CDS             7481..7858
                     /locus_tag="ACHUQ7_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFR1136567.1"
                     /translation="MDGFDNLFDAALAGVNEVILRDMGISAVITSGELEGTHLTGVFD
                     DPESISFVAGGIRLEDSSPCLFVKTADISQLRRQDTLTIGDDSFFVDRITPDDGGCCY
                     IRLRRGSPAPQNNARMRRYDEGT"
     gene            7845..8429
                     /locus_tag="ACHUQ7_003285"
     CDS             7845..8429
                     /locus_tag="ACHUQ7_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1136568.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQTSAWAVNRVAAKIVSVATRQVAQ
                     NTVAGDNQVKGIPLKTVRERVRLLKASPSGKMYARMRVNRGNLPAIKLGTAQVRLARS
                     RHGSNSRHRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIPMSGPL
                     TQAFEDARDRIIAAEMPKQLGYALKQQLRLHLSK"
     gene            8426..>8778
                     /locus_tag="ACHUQ7_003286"
     CDS             8426..>8778
                     /locus_tag="ACHUQ7_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFR1136569.1"
                     /translation="MNRHTQIRQSVLARLREQCGDSATFFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFVRAQAPDSELDTWMENTIFPAMKDIPVLSGLID
                     TMIPLGFNYQRDNEMA"
BASE COUNT         2050 a         2070 c         2690 g         1968 t
ORIGIN      
        1 gcagccttca gggaaaaata tcatcagatg gctgcttaaa aaaagaacaa atggtagtgt
       61 ccgctattgc cagtacacct caaactactc attgccagat gggaactgac cgcagaacag
      121 gctgtcgcac agcagttaaa aaatcaggtg tcaaaaggaa atcttatcga taccggattt
      181 tgtatttttg cccttagtaa gctggcgatg gctttatcca gtacgcttga ctccattcct
      241 ttgtccatgc aacgacagtt cccggattta acgccgcgtc atattgatca tctgaaaatc
      301 cttattgcta agggagcgaa tcagtgcgcg cgtgcggggg ataaattacc ggatttactc
      361 gatgaatata tcagaacaac aactgagtaa tatgatggtg gcagtcacta tcgcactgca
      421 gccactggtc agggtattgc cggtgacggc tgtagaatgg gccgatcaaa attattacct
      481 gcctaaagaa tcgtcatacg gtgaggggga atggaaaacg ctgcagtttc aggtcgccat
      541 catgaactgc atgggcaatg acgagattcg tacagttaac ctgattaaat ctgcccgtgt
      601 tggttataca aaaatggtgc ttggtgtgat cgggtatttt attgagcaca aatcccgcaa
      661 cactcttctt tttcagccga cggactcagc tgctgaagat tttatgaagt ctcacgtgga
      721 agcaacaatt cgtgatgttc cttgcctgaa aaaactttct ccctggctgg gccggaaaca
      781 ccgggataat acgctcacac tgaagcgttt ttcctctggt gtggggttct ggtgtttggg
      841 tggtgctgct gccaaaaact accgtgaaaa atctgtcgat gtggtctgct atgacgaact
      901 ctcctcgttt gaaccggatg tggaaaaaga aggttcgcca acactgcttg gcgataaacg
      961 tattgaaagt tcggtatggc caaaatccat acgcggttca acgccaaaaa ttaaaggctc
     1021 ctgtcagatt gaaaaagctg cgaatgaatc tgcgcatttc atgcggtttt atgtcccttg
     1081 ccctcattgc ggggaggagc agtatctgaa gtttggcgat gatgcgacgt cgtttggcct
     1141 gaaatgggag aagggtaaac cggaaacggt gtattacctg tgtgaacata atggctgcgt
     1201 gatccgtcag tctgaacttg atcagaccgg ggggcgctgg atttgtgaca ataccgggat
     1261 gtggacgtgt gacggtctga cgtttttcag tgcttcgggt aatgaaattc cgccgccgcg
     1321 ctccatcact ttccatatat ggacggcgta cagtccgttc accaccaggg tacagatagt
     1381 ctgtgactgg ctggatgcac tgaaagatcc caacggcgtg aaaacttttg tgaacaccac
     1441 gctgggcgag acctgggaag aggccgtggg cgaaaaactc gatcaccagg tactgatgga
     1501 taaggttgtg ccttacacgg caacggtgcc tgtccgggtg gtttatctga cggcgggcat
     1561 tgactcgcag cgaaaccgtt ttgagatgta tgtctggggg tgggctccgg gagaggaagc
     1621 ctttctggtg gataaaatca tcatcatggg acgtcctgat gaggaagaga cgctgttacg
     1681 tgtggatgcg gcgatcaata aaaaataccg ccatgcagac ggtaccgaaa tgaccatttc
     1741 ccgtgtctgc tgggatatcg gggggatcga tggcgaaatc gtgtatcaga ggtcaaaaaa
     1801 acacggtatt ttccgggtac tgccggtaaa aggtgcctct gtctatggca agccggtgat
     1861 caccatgccg aaaacccgca atcagcgggg tgtgtatctg tgtgaggtgg ggacggatac
     1921 cgcaaaagaa attctctatg cccgaatgaa ggccgatccc acccctgtga atgaggccac
     1981 atcgtatgcc atccgctttc ctgataatcc ggagattttt tcgcagacag aggcacaaca
     2041 actggtggcg gaggaactgg tggaaaagtg ggaaaaagga aagatgcgtc tgttgtggga
     2101 taacaaaaag cggcgtaacg aagcgctgga ctgcctggtg tatgcctatg cggcattacg
     2161 tgtgtccgtg caacgctggc aacttgatct ggctgtgcag ttgcatcccg caaggatgca
     2221 actgcacaac cgacccttga agaactggca gcgaagctgt ccggaggagt gaatggttac
     2281 agtcgctgaa ctgcaggcgc tgcgtcaggc gcgccttgat ttattaaccg gtaaacgggt
     2341 tgtgtcggta cagaaagacg gtcgcaggat cgaatatacg gcagcgtccc tgaatgagct
     2401 taaccgtgcg atcaacgatg cggagtcggt tctgggtacg acccgacgcc gccgaccgct
     2461 gggagtgagg ttatgaaacg aacgcctgtc ctgattgatg tgaacggcgt tccgcttcgt
     2521 gagagtctca gctacaacgg aggcggcgca ggatttggcg ggcaaatggc agagtggtta
     2581 ccaccggcgc agagtgccga tgcggccctg ttgcctgcat tgcgtctggg gaatgcccgg
     2641 gcagatgatc tggtgcgcaa taacggtatc gcggctaatg cggtggccct gcataaggat
     2701 catatcgtcg ggcacatgtt tcttatcagc taccgtctga actggcgttg gctggggatg
     2761 cgggagacca cggcaaaaag ttttgtcggg gaggtggaag cagcctggtc ggaatacgca
     2821 gaaggaatgt ttggcgagat cgacgtggaa gggaaacgca cgtttacgga atttatccgt
     2881 gaaggggtgg gcgtgcatgc ctttaacggc gaaatctttg tgcagccggt ctgggatacg
     2941 gaaaccacgc agttattccg tacgcgtttt aaagccgtga gtccgaaacg ggtggacacg
     3001 ccaggacacg gtatggggaa ccactttctg cgggccgggg ttgaggtcga tcggtatggg
     3061 cgggcgattg cgtaccatat ctgtgaggat gattttccgt tctctggtgc cggacgctgg
     3121 gagcggatcc cgcgtgagct tcccaccggg cgtccggcca tgctgcatat ttttgagccg
     3181 gtggaggacg ggcagacccg tggggctaat cagttttaca gcgtaatgga gcggatgaag
     3241 atgctggact ccctgcaggc cacgcagctt cagtcggcca ttgtgaaggc gatgtatgca
     3301 gccacaatcg aaagtgaact tgataccgaa aaggcatttg aatatatcgc gggggcaccg
     3361 caaggtcagc aggataatcc gcttattaat attctggaga agttcacccg ctggtatgac
     3421 acgaataacg tgacgctggg cggtgttaaa attccgcacc ttttttcctg gcgatgcgct
     3481 gaacctgcag acagcgcagg attcagacaa tggattttcg gcgcttgaac aggcgctgct
     3541 gcggtatatt gcagccggtc ttggcgtttc ctacgaacag ttgtcccgtg attactcgaa
     3601 ggtcagttac tcaagtgccc gcgcctccgc caatgagtcg tggcgctatt ttatggggcg
     3661 gcgaaaattt attgcggccc ggctggccac gcagatgttt tcctgctggc tggaagaggc
     3721 gcttcttcgg gggattattc gtccgccacg ggcgcgtttt gatttttacc aggcgcgttc
     3781 agcctggtca cgggcagagt ggattagctc cggaagaatg gccattgacg ggctcaagga
     3841 ggtccaggaa tcggtgatgc gcattgaggc cggactgagc acgtatgaga aagaactggc
     3901 actgatgggc gaagattatc aggacatttt ccgccagcag gtcagggaat ccgcagagcg
     3961 cgaaaaagcc ggactctcac gtccggtgtg gatagcccag gcgtatcagc agcagatagc
     4021 ggagagtcgc aggccggaag aggagacaac atcacgtgag acgtaatctt tcacatatta
     4081 tcgctgcggc attcaatgag ccgctgcttc tggagcccgc ctatgcgcgg gttttctttt
     4141 gcgcgctcgg gcgtgagatg ggcgcagcaa gcctttcggt accgcaacag caggtacagc
     4201 ttgacgctcc cgggatgctg gctgaaacgg acgagtacat ggccggaggc aaacgacctg
     4261 cccgtgttta ccgggtggtg aacggtattg ctgtactgcc ggtgaccggc acgctggtac
     4321 accggctggg tggcatgcgg ccattttccg gaatgactgg ctatgacggc attgtcgcct
     4381 gtcttcagca ggcaatggcg gacactcagg tacggggcgt actgctggac attgacagtt
     4441 ctggcgggca ggccaccggc gcgtttgact gcgctgacat gatttaccgt ctccggcagc
     4501 tgaagcctgt ctgggcactg tgcaatgaca cggcctgttc agccgccatg ttactggcat
     4561 cggcctgctc ccgacggctg gttacccaga catcccgtat cggctccatt ggggtgatga
     4621 tgggccatgt cagctatgcc ggtcatctgg cacaggccgg tgtggatatc acgctgattt
     4681 acgccggatc gcacaaggtg gatggcaatc agtttgaatc cctgccagca aaggttcgcc
     4741 aggacatgca gcagcgcatt gatgcggcgc gccggatgtt tgctgaaaaa gtggcgatgt
     4801 ataccggttt atctgtggat gcggttatgg gaacagaggc cgccgttttt gaaggtcagt
     4861 ccggtattga ggccgaactg gcggatgaat taatcaatgc gtcggacgcc atcagcgtga
     4921 tggctgcgac gctgaacaca cacgatacag gaggcactat gccgcaatta actgcaacgg
     4981 atgctgccgc gcaggagaac cagcgagtga tggggatcct gacgtgtcag gaagcgaaag
     5041 gacgtgaaca actggccacg atgctggcag ggcaacaggg tatgagcgtt gagcaggccc
     5101 gggcgattct ggccgtggcg gcaccgcaac aatctggtgt atctgcgcag agtgaagccg
     5161 atcgcattat ggcatgtgaa gaggcgaaag gacgtgaaca actggcggca acgctggcgg
     5221 cgatgccgga gatgacggtg gaaaaagccc gtccgattct ggctgcttca ccgcaggcga
     5281 atgccggacc ctcacttcgt gatcagatta tggccctgga tgaggcaaaa ggggcagagg
     5341 cgcaggctga aaaactggcg gcctgtccgg ggatgaccgt ggagaatgcc cgggctgtac
     5401 tggcggcagc ctcaggcagg gcagaaccgg tttctgcatc cacaaccgcc atgtttgaac
     5461 atttcatggc gaaccattca ccggcagcgg tacagggtgg cgtgccacag acgtcagcag
     5521 acggtgatgc ggacgtgaaa atgctcatgg ccatgccatg aagtcagtgc tgaccatcaa
     5581 tatgaggttt ttacaatatg gtaacgaaaa ccatcactga acagcgtgcg gaagtacgta
     5641 tttttgccgg taatgatccg gcccataccg ccacaggctg cagcgggatt tcctcggcaa
     5701 caccggctct gacgcccctg atgctggatg aggataccgg gaaactggtg gtctgggacg
     5761 gacagaaggc aggccatgtg gttggcatac tggtactgcc gcttgaaggt acagagacgg
     5821 tgctgacgta ttacaagtcg gggacctttg cgacggaggc catccactgg cctgagagag
     5881 tggatacaca caaaaaggcg aatgcctttg ccggcagtgc cctgagtcac gcagcgctgc
     5941 cgtaacattt tatcaggtca ctgcggtagc ctgactgatt tctgaatgaa aggaactgat
     6001 ttatgggatt gtttacgacc cgccagttac tcggttatac cgaacaaaaa gttaaatttc
     6061 gtgcgctgtt tctggagctg tttttccgcc gtacggtgaa tttccatacc gaagaggtaa
     6121 tgctggacaa aattaccgga aaaacgccgg tggcggccta tgtctccccg gttgttgaag
     6181 gaaaggtgtt gcgtcaccgt ggcggtgaaa ctcgtgtact gcgcccgggc tacgttaagc
     6241 cgaaacacga atttaattac cagcaggcgg tggagcgtct tcccggtgaa gatccggccc
     6301 agctgaacgg cccggcttac cgtcgcctgc gtatcatcac cgataacctc aaacaggaag
     6361 agcacgccat tgtccaggtg gaagagatgc aggcggtgaa tgccgtgctg tatggcaaat
     6421 acaccatgga aggagagcag tttgaaactg ttgaggtcga ttttggccgg tcggtgggaa
     6481 ataacattac gcaggcagga gggacggagt ggagcgctca ggaccgcgat acctttgatc
     6541 cgacgcatga tctggatgcg tactgtgatt ttgcttccgg caccatcaat attgccatta
     6601 tggatggtaa ggtctggcgt ctgctgaatg gctttaagct gttccgcgaa aaactggata
     6661 cccgtcgtgg ctcaaattca cagctggaaa cggcagtgaa agacctgggg gcggtggtgt
     6721 ccttcaaagg gtattatggt gatctggcaa tcgtggtggc aaaaacgtcc tatgtggcag
     6781 aggacggtac cgaaaaacgt tatctgccgg acggcacact ggtactggga aatacggccg
     6841 cagagggtat tcgttgctac ggtgccattc aggatgcgca ggcattgtct gaaggcgtgg
     6901 tggcgtcttc ccgttatccg aaacactggc tgaccgtggg tgatccggcg cgtgaattca
     6961 ccatgacgca gtccgcgccg ctgatggtgc tgccggatcc ggatgagttt gtggtggtgc
     7021 aggtgaaata atccgtgagc gggagctaaa tgctcccgtt tttcattcaa tcaggagcgg
     7081 gtatggctac gaaagaagaa aacctgaaac gtctttgtga gctggctgag aggctggggc
     7141 gtgaaccgga tgtctccggt agtgcggcag atattgcgca gcgagtggcg gaactggagg
     7201 aagagcttgg agatgcgggg gaggtcgcag agccggatac gtactcacca tcgaaagatg
     7261 atccgaccgc gcataaaggc gagtcagttc ctgaacagcc aggcattgtt accggagacg
     7321 aaacggggca ggttacggtg gtggcactgg cgacactgca tacaaaatgt aatggttcag
     7381 tgatatttgt ttcaccggga acgtcgtttc gtgtttctgc tgaggtggct gccagcatgg
     7441 cggcaggtgg tctggcaaaa aggcagtaac aggagatgtc gtggacggtt tcgataatct
     7501 gtttgatgct gcgcttgccg gtgtcaatga agtcattctc cgtgatatgg ggatcagcgc
     7561 cgtcattacg tccggagaac ttgaggggac acacctgaca ggggtttttg atgatcccga
     7621 aagtatttct tttgttgccg gggggatccg gcttgaggat tcctcaccgt gcctgtttgt
     7681 gaaaacggca gatatatcgc agttacgccg tcaggatacg ttaactatcg gggatgattc
     7741 tttctttgtg gatcgtatta ctccggatga cgggggatgc tgttatatcc ggttaaggcg
     7801 gggcagtcct gccccacaga ataatgccag aatgaggcga tatgatgaag ggacttgaga
     7861 atgccatccg caatctgaac agcctcgata cccgcatggt gccacagacc agcgcatggg
     7921 cggtaaaccg tgtggcggca aagattgttt ctgtcgccac acggcaggtt gcgcagaata
     7981 ccgttgccgg ggataaccag gtgaaaggta ttcccctgaa aacggtcagg gagcgtgtca
     8041 ggctgcttaa agccagcccc tcaggaaaaa tgtatgcccg catgcgtgtt aaccggggta
     8101 acctgcccgc cattaagctg ggtaccgcac aggtcagact ggcccgctcc cggcatggca
     8161 gcaactcacg tcatcgtggc agcgtactga aagtggggaa ataccttttc cgggatgcgt
     8221 ttattcagca actggcgaat ggtcgctggc atgtgatgcg gcgtattgac ggcaaaaatc
     8281 gttaccccat tgatgtggtg aaaatcccga tgtccggacc gctgacacag gcatttgaag
     8341 atgcccgcga ccgcatcatt gctgcggaaa tgccgaaaca gctggggtat gccctgaaac
     8401 aacaactgag gttacatctg agtaaatgaa ccgacacaca caaatccgtc agagcgtgct
     8461 ggcacgcctt cgggaacagt gtggagacag cgccacgttt tttgacgggc ttccggcatt
     8521 tattgatgcg caggaactgc ccgccgtggc ggtgtggctg agtgatgcgc agtacaccgg
     8581 aaaaatgacg gatgaggacg actggcaggc tgttctgcat attgccgttt tcgtccgtgc
     8641 acaggcgccg gattctgagc tggatacgtg gatggagaac accatatttc cggccatgaa
     8701 ggatattccg gtgctttccg gactcatcga caccatgatc ccactcggtt ttaactatca
     8761 acgtgataat gagatggc
//
LOCUS       DAZPCS010000148         8768 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZPCS010000148 DAZPCS010000000
VERSION     DAZPCS010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8768
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.149"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..439
                     /locus_tag="ACHUQ7_003287"
     CDS             <1..439
                     /locus_tag="ACHUQ7_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFR1136570.1"
                     /translation="QKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKASKDIQ
                     FAVQSGPMLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYA
                     KAKLNVEQLLYLGGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            complement(586..2748)
                     /gene="uvrD"
                     /locus_tag="ACHUQ7_003288"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(586..2748)
                     /gene="uvrD"
                     /locus_tag="ACHUQ7_003288"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFR1136571.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAEI
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(2832..3548)
                     /gene="yigB"
                     /locus_tag="ACHUQ7_003289"
     CDS             complement(2832..3548)
                     /gene="yigB"
                     /locus_tag="ACHUQ7_003289"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFR1136572.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(3548..4444)
                     /gene="xerC"
                     /locus_tag="ACHUQ7_003290"
     CDS             complement(3548..4444)
                     /gene="xerC"
                     /locus_tag="ACHUQ7_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFR1136573.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(4441..5148)
                     /locus_tag="ACHUQ7_003291"
     CDS             complement(4441..5148)
                     /locus_tag="ACHUQ7_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HFR1136574.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPGFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(5145..5969)
                     /gene="dapF"
                     /locus_tag="ACHUQ7_003292"
     CDS             complement(5145..5969)
                     /gene="dapF"
                     /locus_tag="ACHUQ7_003292"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFR1136575.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6006..6209)
                     /locus_tag="ACHUQ7_003293"
     CDS             complement(6006..6209)
                     /locus_tag="ACHUQ7_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR1136576.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            6368..7876
                     /locus_tag="ACHUQ7_003294"
                     /pseudo
     CDS             6368..7876
                     /locus_tag="ACHUQ7_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            7873..>8768
                     /locus_tag="ACHUQ7_003295"
     CDS             7873..>8768
                     /locus_tag="ACHUQ7_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFR1136577.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRAGLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTLTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVELATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTPDRQWSKWIDLPGIEPEQ"
BASE COUNT         2036 a         2390 c         2252 g         2090 t
ORIGIN      
        1 gcagaaggtg gcgttaaatc tcgcttcagg tgaagggaat ttctttatcc gtcctggcgg
       61 cgtgttttat gtcgcgggag ataaagtcgg catcgttcgt ctggatgctt ttaaagccag
      121 taaagatatt cagtttgcgg tgcagtcagg accaatgttg atggaaaacg gtgtaattaa
      181 tccgcgtatt catcccaacg tcgcctcacg taaaattcgt aacggtgttg ggattaataa
      241 acatgggaat gccgtgtttt tgttgagcca gcaggcgaca aatttttatg attttgcctg
      301 ttatgccaaa gcgaaactga acgttgagca gctgctttat cttggtggca cgatttcaca
      361 tatgtatatg aaaggcgggg caatcccgtg gcaacgctat ccttttgtca ccatgatttc
      421 cgtcgagcgg aaaggttaga atgatagctt tgtgtcagat actaaatatg ctgctgatgc
      481 cggatgcggc gtaaacgcct tatccggcct acatgacgtt gcaatttatt gaatctgaat
      541 gattttttag gccaaataag gtgcgcagca ccgcatccgg caacgttaca ccgactccag
      601 ccgggcgtaa gctgccacca gccatttgat tccctggccc tggaatgcca cctgcaaacg
      661 gctatgttca ccgctgcctt ccatattgac gatggtgcct tcaccaaact tagcgtggcg
      721 tacacgctgg ccgagcttgt agccgctgtc gttctcgacc atcggcgtac ccatccgctg
      781 atggctgacc gggcggctta ccgtggcgcg caggcgcacc tcttccacac actcttccgg
      841 caactcgcca ataaagcgcg acgggcgatg gtaaacctct ttgccataca gacggcgagt
      901 ttccgcgtag gtcagcgtca gtttctgcat cgcgcgggtt acgccaacgt aggccagacg
      961 gcgttcttct tccagacgcc cgccttcatc cagcgacatc tggcttggga acatgccctc
     1021 ttccataccg acgataaaca cctgcgggaa ctccagacct ttcgccgagt gcatggtcat
     1081 cagttgtacc gcatcctgcc aggtatccgc ctgcccttcg cctgcttcca gcgccgcatg
     1141 ggataggaac gcctgcagcg gcattaaatc ttcgtcttct tcgttgtagc tgaactggcg
     1201 cgttgccgtc accagttcct ctaagttttc gatacgcgtc tgaccttttt cgcccttctc
     1261 ctgctcgtac atggtacgca ggccggagtc tttaattacc cggtcagtct gtacatgcag
     1321 cggcatatcg gcagtttcct gcgctaaggc gtcgatcagt tccataaacc gctgtaaggc
     1381 gctggcagca cgtccggcga gggctttttc ctgcaacagt tcacgacatg cctgccagag
     1441 tgttaactgg cgatcgcgcg atgtctgacg taccacgtcc agcgtccggt caccaatacc
     1501 ccgcgttggc gtattcacca cacgctcaaa ggccgcgtcg tcgttacggt tggcaatcag
     1561 gcgcagatac gagagcgcat ctttgatttc ctggcgttcg aagaagcgca tcccgccgta
     1621 aatacggtac ggcatactcg cctgcaacag tgcttcttcc agcacacgcg actgggcgtt
     1681 actgcggtag agaatggcgc actcggcaag cgccccgccg ttgtcctgcc aggttttgat
     1741 gcggttaacc acaaaacgcg cttcatcgag ttcgttaaaa gcgcaataga gggaaatagg
     1801 ctcaccgtcc gcgccatcgg tccacagttt tttacccaga cgcccgttat tgttttcaat
     1861 cagggcgtta gcggcgctca gaatattgct ggtagagcgg tagttttgct ccagacgaat
     1921 aatttcggca ccggggaaat cattaaggaa acgctgaata ttctccacct gcgccccgcg
     1981 ccagccgtag attgactggt cgtcatcacc gacgatcatc actttgccgg tgtcgcccgc
     2041 cagcaggcgg atccacgcgt actgaatgtt gttggtatcc tggaattcgt ccaccaggat
     2101 attggtaaaa cgttcgcggt agtgttgtag gatatgcggc ttgttaagcc acagttcgtg
     2161 agcgcgcagc agcagctcgg cgaagtccac caggcccgcg cgatcgcacg cttcctgata
     2221 cgcctgatac accttctgcc aggtctgctc caccggatta ccgtagcttt gaatatgatg
     2281 cggacgcagg ccttcatctt tctggctgtt gatgtaccac attgcctggc gcggcggcca
     2341 ctgcttctcg tcgaggttca tggctttgat cagacgctta agcaggcgta gctggtcttc
     2401 gctatcgagg atctggaaat cctgcggcag attggcgtcc atatggtgcg cacgcagcag
     2461 gcggtgcgcc agcccgtgga aggtgcctac ccacatgccg ccctggctgg tgcccatcag
     2521 ttgtccgata cgatgacgca tctccgccgc cgctttgttg gtaaacgtca cagccataat
     2581 cgagtatggc gagcagtttt ccacgctcat cagccaggcg atacgatgca ccagtacgcg
     2641 cgtcttacca ctgcccgcgc ccgccagcac cagaaggttg ctgcgtggcg cggccaccgc
     2701 ttcgcgttgt ttgtcattaa ggctgtcgag caggtaagaa acgtccattg gcaccgccgc
     2761 gtaaaaatag gttgggcgga gaagcgcaac gccctccgcc aaaaagctgg gtatatatac
     2821 agatttgctg attatatcag cgaggtcaga gatgccaacc gggaaatttc cagatgtggc
     2881 agtaaacggc tgtcccaggt ttgcatcaga tcgccatttt ccggtctgat ccaacaagcc
     2941 tgcattccgc tgcgaattgc cccacccacg tcagtggtga gatcgtcccc aacatgtaag
     3001 atctcgccga tcggcacgtt gagtttttcc gcagccaaaa agtacatatc gctgaacggt
     3061 tttgagcgcc cgtgcgggcc agcgcgcagc acaaactcaa aataatcccc caaaccaaac
     3121 agttccggct gggcgttacc gttggtgatc gccaccagcg gccatttctt cgccagctgt
     3181 tttaaggtgt cgtgagtttg ctgcgggacg tcgattcggc tgcgccattt ggcaaagtta
     3241 atcattgctg cgtgtgcgcc tgcactggct tcttcggcac tcagcccggc gtcgagcatc
     3301 gcttgttcaa tcgaacgaaa acgccaacgt gtcacgtcgt gataaatctc gggttccgct
     3361 tcccgtaccg cctggcgcag gcgttgcaga tcttcattct ggaagctgcg cagcgccgga
     3421 tgataatttt gcacaaaggt aagcgcctct cgctcggtgc gcaaaatcac cggacggtta
     3481 tcgtaaaggg tatcatccag gtcaaaggtg agcgccgaga tgcgccccaa aggccggtaa
     3541 aaacgcatta tttcccccgt ttggcgcgtg gatgcgccgc atcgtacacc gaggcaaggt
     3601 gttgaaaatc aagatgagta tagatttgcg tggtggagag gttggcatga cccagtagct
     3661 cctgcacacc acgaagatcg ccgctcgact ccagcatatg cgtagcgaac gagtgacgta
     3721 atttatgtgg gtgaacgtga ttattcagcc cttgttttat gccccattcg gcaaagcgtt
     3781 tctgcacatt acgcgcggag atacgcttgc ccagtttcga cagaaaaagc gcgtcgtctt
     3841 cgctaccaaa caggtcgcgc aaatcaagcc agtgctcaat ccacgccaca gcgttgcgac
     3901 caatcggcag gcggcgctct ttgctgcctt tccccatcac ccacacttcg ccggactcca
     3961 ggtcgaggtg tttgatatcc agccccacca gctcggaaag acgcagaccc gcgccgtaca
     4021 tcacttccag cattgcacgg tcgcgtacag cgaggggatc gttgatatca atatccagca
     4081 gccgattcat atcgtcgacg tcgatatttt tcggcagatg acgcggcgct ttcggtgccg
     4141 aaactccttt cgccgggtta gctttgagtt cgttctggct gaccagccag tcaaaaaagc
     4201 tacgtagcgc agaaagccgt aacgccagac ttgctgctcc cagcccttta cggcgactgc
     4261 gtacagcaaa attgcgtacc atcgctgcat cgcattgttg ccagctttgc aggccgtttt
     4321 cgctggcaaa attgatgatc gcctcaagct gacgctggta gttaagtagg gttatcgggc
     4381 taagctgacg ctccacgctc agataacgta ggtagcgttc tacatcggtg tgtaaatcgg
     4441 tcatacgcgt tcaatccaac gctccagaag ctccggcaac atcagcgcaa tttcatgaag
     4501 taactgcgtt ccctgccctt gttgatagtg actggcatcg cgactggtaa acagcacaac
     4561 acccaaatca gcatcgcttc ccagcatcga catcgccacc gatcccaccg ctttcgcttc
     4621 cggtagcacc accagcagct ccggtccgtt aagcggccca agatagtgct gttcctgccc
     4681 caaacgctga atacgcagcg gttcgaaaga ctgacggctt aatgccagat gagtgtggtt
     4741 cgacggcgca cctaagcgcc agcgatctgg aaacaggcgc agactcgcac cagctaggcc
     4801 gagatcgcgc gcccagcggt gaaagcgcat cagcatatcg tcgagactgc tggcggcggt
     4861 gagactgcgc tgcaggtaga gtaggcgata aaacagccct tcgttggcga tagcctgctc
     4921 catcaacaac gccatgttct cttccagaac atgaatgtga ttacgtgcgc gggccatgtg
     4981 ccactcgacc aacgaaacgg tgccgcgtac cggatgcggc acacgtatcg cttctactgc
     5041 gcgcgcattg cggataaaaa acccaggatt tttaatcaga taatcgacaa ccgcccggtc
     5101 atcaagctcc gtgagtgttt cctgcagttc ttcccctggt tgcttcatag atgaataaat
     5161 ccatcgtaga catgtaccgc cgggccagtc atatataacg ggtgacccgg acctttccag
     5221 gcgatatcaa gacgaccgcc ggggagttcc acgcgtactt cttcgtccag caaaccttgc
     5281 tgaatcccta ccgccaccgc cgcacacgcg ccgctgccgc aggcctgggt ttctcctgcc
     5341 ccacgctcat aaacgcgtaa acgaatatgc tcgcgcttaa ccacttgcat aaaaccgata
     5401 ttggcgcgct ccggaaaacg ctcgtgactt tccagaacag gaccaagcgt ttctaccgcc
     5461 gcggtatcga catcatcgac ctgaatcacg caatgcggat ttcccatcga caccacgccg
     5521 cataagattg tctgctcggc ggcgcgcata atataggtct tttccgcttt gttagcgcga
     5581 aacggcacgg cggaaggttc gaagttgggt tcgcccatat ttacgcggac cagatcatca
     5641 tcggtgacgg tcagaaccat ccgcccgttg gcggtgctga cgcggatatc acgcttattg
     5701 gtcagtcctt tcagacgcac aaaacgggca aagcagcgcg caccgttacc gcactgcgcc
     5761 acttcactgc catcagcatt gaaaatgcga tagtgaaaat ccagttcagg atcatacggc
     5821 ggctcaacca ccagcagctg gtcaaaccct acccccaggt gccgatccgc caggcgacga
     5881 atcagctccg gtgaaaaaaa gacattctgc gttaccgcgt cgacgaccat aaaatcgttg
     5941 ccaaggccat gcattttcga gaactgcatc atttactcca atcacgcggg tacagaaact
     6001 gacttttagt aattcacctg ggatggacca tcgccagtgg cgcgatcgtt tttatccggc
     6061 accgtggatt gcgtttgcgt ctctaccggt ttggtcggcg gcggtgcgtt tttatctgca
     6121 ggcgggaaat agagcggacc tttcagaccg cagcccgtca ggctgaagag agtaagtaat
     6181 acagtgagtg ccttaaacac gtttttcatt atggattgcc tgtaaagttc atcgctttct
     6241 gctttctatc atcgcaggag aaggcgtaaa agcaagcgtt tagcgactct ttatgaagtc
     6301 tgaaaagtga ggaaatgctg cgagtataac gcttactcgt gaggatttat gttctgctgg
     6361 cagtttcgtg atcaacacct cgcgccagga tggctttatg cactatcgca atgtggtttg
     6421 tttgttgtct tgttcattgt ttttatcgtc ggcatgggga tgtcggcttt ccgcacctga
     6481 acataatatt taccagaaac agggaaaagg cgtggtgtac ctgcgccctt ataaagaaac
     6541 aaatctttct ctacctgacg tgaattacaa gagtttgcgt cggttaccca atttattaat
     6601 agacccgaca acactagacg aatgggataa agaaccgcct ctgactgatt tgacaaccga
     6661 ctacctctat gagggcgcac aggcctggta tcctcattat tcatggcata gcgatggtcg
     6721 caatatcctc tacgcgggtg aagttgtgca aaaccctccc ggcacacctc ccgtcgatgt
     6781 cgcctcgttc aaggcatggg gtgattttgc cgcagataaa cacagtctct attttgaagg
     6841 caagcgcacc gatgacaatg gtggtggaaa tagcttggat atcaaaacgc tgcatcaggt
     6901 ggaattccgg cctccctggg atcctgactt actggggctg atattacgtg acgctaattt
     6961 cttgtatatc aatgggcatc gcctcgctga ccccgagagt ttccgtgtgc tggcgcagaa
     7021 gtcctgggat cagcgaggaa agttttccac cacgtttaac ccctgcattg ccgtaccatt
     7081 tggcccctgg gataccctgg ctcgtacacg gaccaaaatc ctgctcaacg gcgaacagct
     7141 tgatgccgac ccggacacct tttccgtcgt gcgctggatg cccggctcac tcttgacctg
     7201 gcgtgataaa aacgggctgc agcgtaaagt cctcgacaag gaaaatctgg cgtgggatga
     7261 agatttaaca aagcactgtc tggatttttc tctgctggaa aagaaagtgt tctggcgtaa
     7321 aggacctgct tgtaaacagg aagaattacc cggactcgat ccggagcagt ttcaacccat
     7381 cagtgatgct gtcgcccagc atcaggactc gctttatacc atcatcgaaa cagagtctgg
     7441 taaccgtaag ctggagatcg tgaaacttga tgatcccaat cttattatca acaaacgttt
     7501 caacgccggg aaacgccacg gctatttact tacgcgtgcc gaagggtagc cataccattc
     7561 cggtttacac gtgtttgaat ctgacggacc gctgatctta ctggataacc gttctccgga
     7621 tgaacgcgaa gcccatctta atgaccatcc ctttttgcgc agatggtatg cccgcgataa
     7681 ccgctacatt tacagctttg atggcgcgca gctctggcga taccgcaccg ctgatccgaa
     7741 acaagttcgc ttaatctgga aggaacaaca ttcgggatat gtctatggcg taaattacaa
     7801 aacgggatat ctggacggaa aaattactga tgacggcgaa tttattcctg ccccgcgcaa
     7861 tgaggcgaca aaatgaaggg tcaaataatc acagttgcac tcatcctgtt gggacacaca
     7921 tttctgacgc ctgctgtaca ggccataggg ttcgactatt acaatgatca cggtgttatg
     7981 tcttacggta aaggttattg gggggataag aaaatcattg cccagtttcc ggagatgagc
     8041 agggccggtt tgcgccttgt gaaaaatatc tctgggaaaa cgggttatat accttccgat
     8101 gaaataagca tagaaaatga ggaataccac tgggtgacag acggccgcgt tattctctgg
     8161 cgtggcaaaa tcgttagcaa tccaccggga acactcactg tcgatattgc cagctttcag
     8221 gctatgggcc gcttcgcggt cgataaatat agcctctatt ttgacggaca gcgcaccgaa
     8281 agtaatagcg gcgcgtcacg tgttgagctg gcgacactaa aagctatcga aggtaactct
     8341 accacgctgg tggatagtaa aaatctctac ttgtccggtc gacgtcaggg gagcagcagt
     8401 gatgttactg tattagaaaa aagatggtgg ggtattaatc cacgtcttat gagtgtaaac
     8461 agaaattcat attccaatga tctgcttatc cgcagtgggc agaatattta tttaaatggc
     8521 gttcacctta cggcgaatgc agactcattt gagataattc gctggatacc tcattcactg
     8581 ctggtttttc gcgacaataa gggtctgcat cgttatccct ttggtcaatt atcaggcaaa
     8641 gcgatacccg tagatgatga cgtctctttt gaagtagggg aaagtcgcgt tcgctggcgt
     8701 aaacagctca cgcccgaccg tcagtggagc aagtggatag acctaccagg tattgaacct
     8761 gaacaatt
//
LOCUS       DAZPCS010000149         8664 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZPCS010000149 DAZPCS010000000
VERSION     DAZPCS010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8664
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.150"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(330..593)
                     /gene="rpsT"
                     /locus_tag="ACHUQ7_003296"
     CDS             complement(330..593)
                     /gene="rpsT"
                     /locus_tag="ACHUQ7_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFR1136578.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            696..914
                     /locus_tag="ACHUQ7_003297"
     CDS             696..914
                     /locus_tag="ACHUQ7_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFR1136579.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICSMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            922..1863
                     /gene="ribF"
                     /locus_tag="ACHUQ7_003298"
     CDS             922..1863
                     /gene="ribF"
                     /locus_tag="ACHUQ7_003298"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFR1136580.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1906..4722
                     /gene="ileS"
                     /locus_tag="ACHUQ7_003299"
     CDS             1906..4722
                     /gene="ileS"
                     /locus_tag="ACHUQ7_003299"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFR1136581.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWLPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4722..5216
                     /gene="lspA"
                     /locus_tag="ACHUQ7_003300"
     CDS             4722..5216
                     /gene="lspA"
                     /locus_tag="ACHUQ7_003300"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFR1136582.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            5303..5752
                     /gene="fkpB"
                     /locus_tag="ACHUQ7_003301"
     CDS             5303..5752
                     /gene="fkpB"
                     /locus_tag="ACHUQ7_003301"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR1136583.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5754..6704
                     /gene="ispH"
                     /locus_tag="ACHUQ7_003302"
                     /gene_synonym="lytB"
     CDS             5754..6704
                     /gene="ispH"
                     /locus_tag="ACHUQ7_003302"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFR1136584.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6770..7684
                     /gene="rihC"
                     /locus_tag="ACHUQ7_003303"
     CDS             6770..7684
                     /gene="rihC"
                     /locus_tag="ACHUQ7_003303"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFR1136585.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            7851..>8664
                     /gene="dapB"
                     /locus_tag="ACHUQ7_003304"
     CDS             7851..>8664
                     /gene="dapB"
                     /locus_tag="ACHUQ7_003304"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFR1136586.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLN"
BASE COUNT         1980 a         2157 c         2460 g         2067 t
ORIGIN      
        1 aggtctaatg aggagatatt tatgaatggt taaagtatat aggaatttaa ttcaaaaata
       61 gagtggtctt aagtttaata cctcaatgtg tatcacaata tccatattct ttgtggggga
      121 gtctggagat tgagtagata ttcttgttca gaatgtatca gccgatggtt ctacgattct
      181 taagccacga agagttcaga tagtacaacg gcatgtctct tttgactatc tggcaaccgg
      241 cagtgtgttc tctcacgcat cacaaaagca gcaggcataa aaaaacccgc ttgcgcgggc
      301 tttttcacaa agcttcagca aattggcgat taagccagtt tgttgatctg tgcagtcagg
      361 ttagccttat gacgtgcagc tttgtttttg tggatcagac ctttagcagc ctgacggtcc
      421 acgatcggtt gcatttcgtt aaatgctttc tgtgcagcag ctttgtcgcc agcttcgata
      481 gctgcgtata ctttcttgat gaaagtacgc atcatagagc gacggcttgc gttgtgctta
      541 cgagcctttt cagactgaat ggcgcgcttc ttagctgatt tgatattagc caaggtccaa
      601 ctcccaaatg tgttctatat ggacaattca aaggccgagg aatatgccct tttagccttc
      661 ttttgtcaat ggatttgtgc aaataagcgc cgttaatgtg ccggcactcg ttacgtagtg
      721 atggcgcagg attctaccag cttgcggggt gtgaatacag cttttccgcg ataaaaattg
      781 cagcaggcgg tcagtttctt cccgtgattt gctccatggc aatgaaaagc cacttctttc
      841 tgatttcggt actcaatcgc cggttaacct tgaccgctgt acaaggtata ctcggacgat
      901 tttcactgtt ttgagccaga catgaagctg atacgcggca tacataatct cagccaggcc
      961 ccgcaagaag ggtgtgtgct gactattggt aatttcgacg gcgtgcatcg cggtcatcgc
     1021 gcgctgttac agggcttgca ggaagaaggg cgcaagcgca acttaccggt gatggtgatg
     1081 ctttttgaac ctcaaccact ggaactgttt gctaccgata aagccccggc aagactgacc
     1141 cggctgcggg aaaaactgcg ttaccttgca gagtgtggcg ttgattacgt gctgtgcgtg
     1201 cgtttcgaca ggcgttttgc ggcgttaacc gcgcaaaatt tcatcagcga tcttttggtg
     1261 aagcatttgc gcgtaaaatt tcttgccgta ggtgatgatt tccgctttgg cgctggtcgt
     1321 gaaggcgatt tcttgttatt acagaaagct ggcatggaat acggcttcga tatcaccagt
     1381 acgcaaactt tttgcgaagg tggtgtgcgt atcagcagca ccgccgtgcg tcaggcgctg
     1441 gctgatgaca atctggctct ggcagaaagt ttactggggc acccgtttgc catctccggg
     1501 cgtgtagtcc acggtgatga attagggcgc actataggtt tcccgacggc gaatgtaccg
     1561 ctacgccgtc aggtttcccc ggtgaaaggg gtttatgcgg tagaagtgct gggcctcggt
     1621 gaaaagccgt tacccggcgt ggcaaacatc ggaacacgcc caacggttgc cggtattcgc
     1681 cagcagctgg aagtgcattt gttagatgtt gcaatggacc tttacggtcg ccatatacaa
     1741 gtagtgctgc gtaaaaaaat acgcaatgag cagcgatttg cgtcgctgga cgaactgaaa
     1801 gcgcagattg cgcgtgatga attaaccgcc cgcgaatttt ttgggctaac aaaaccggct
     1861 taagcctgtt atgtaatcaa accgaaacac ggaaccgaga atctgatgag tgactataaa
     1921 tcaaccctga atttgccgga aacagggttc ccgatgcgtg gcgatctcgc caagcgcgaa
     1981 ccgggaatgc tggcgcgttg gactgatgat gatctgtacg gcatcatccg tgcggctaaa
     2041 aaaggcaaaa aaaccttcat tctgcatgat ggccctcctt atgcgaatgg cagcattcat
     2101 attggtcact cggttaacaa gattctgaaa gacattatcg tgaagtccaa agggctttcc
     2161 ggttatgact cgccgtatgt gcctggctgg gactgccacg gtctgccgat cgagctgaaa
     2221 gtagagcaag aatacggtaa gccgggtgag aaattcaccg ccgccgagtt ccgcgccaag
     2281 tgccgcgaat acgcggcgac ccaggttgac ggtcaacgca aagactttat ccgtctgggc
     2341 gtgctgggcg actggtcgca cccgtatctg actatggact tcaaaactga agccaacatc
     2401 atccgcgcgc tgggcaaaat catcggcaat ggtcacctgc acaaaggcgc gaagccggtg
     2461 cactggtgcg tagactgccg ttctgcactg gcagaagcgg aagttgagta ttacgacaaa
     2521 acttctccgt ccattgacgt cgctttccag gcggtcgatc aggatgcgct gaaagcgaaa
     2581 tttggcgtaa gcaacgttaa cggcccaatc tcgctggtga tctggaccac tacgccgtgg
     2641 actctgcctg cgaaccgcgc aatctctatt gcacctgatt tcgactatgc gctggtgcag
     2701 atcgacggtc aggccgtgat tctggcgaaa gatctggttg aaagcgtaat gcagcgtatc
     2761 ggcgtgaccg attacaccat tctcggcacg gtaaaaggtg cagagcttga gctgctgcgc
     2821 tttacccatc cgtttatggg cttcgacgtt ccggcaatcc tcggcgatca cgttaccctg
     2881 gatgccggta ccggtgccgt tcacaccgcg cctggccacg gcccggacga ctatgtgatc
     2941 ggtcagaaat acggcctgga aaccgctaac ccggttggcc cggacggcac ttatctgccg
     3001 ggcacttatc cgacgctgga tggcgtgaac gtcttcaaag cgaacgacat cgtcgttgcg
     3061 ctgctgcagg aaaaaggcgc gctgctgcac gttgagaaaa tgcagcacag ctatccgtgc
     3121 tgctggcgtc acaaaacgcc gatcatcttc cgcgcgacac cgcagtggtt cgtcagcatg
     3181 gatcagaaag gtctgcgtgc gcagtcactg aaagagatca aaggcgtgca gtggctcccg
     3241 gactggggcc aggcgcgtat cgagtcgatg gttgctaacc gtcctgactg gtgtatctcc
     3301 cgtcagcgca cctggggcgt accgatgtca ctgttcgtgc acaaagacac ggaagagctg
     3361 catccgcgta ccctcgaact gatggaagaa gtggctaaac gcgttgaagt tgacggcatc
     3421 caggcgtggt gggatctcga tgcgaaagag atcctcggcg acgaagctga ccagtatgtg
     3481 aaagtgccgg atactctcga cgtatggttt gactccggat ctacccactc ttctgttgtt
     3541 gacgtgcgtc cggaatttgc cggtcacgca gcggacatgt atctggaagg ttctgaccag
     3601 caccgtggtt ggttcatgtc ttccctaatg atctccaccg cgatgaaagg caaagcgccg
     3661 tatcgtcagg tactgaccca cggctttacc gtggatggtc agggtcgcaa gatgtctaaa
     3721 tccatcggca acaccgtttc gccgcaggat gtgatgaaca aactgggcgc ggatattctg
     3781 cgtctgtggg tggcatcaac cgactacacc ggtgaaatgg ccgtttctga cgagatcctg
     3841 aaacgtgctg ccgatagcta tcgtcgtatc cgtaacaccg cgcgcttcct gctggcaaac
     3901 ctgaatggtt ttgatccagc aaaagatatg gtgaaaccgg aagagatggt ggtactggat
     3961 cgctgggccg taggttgtgc gaaagcggca caggaagaca tcctcaaggc gtacgaagca
     4021 tacgatttcc acgaagtggt acagcgtctg atgcgcttct gctccgttga gatgggttcc
     4081 ttctacctcg acatcatcaa agaccgtcag tacaccgcca aagcggatag cgtggcgcgt
     4141 cgtagctgcc agactgcgct gtatcacatc gctgaagcgc tggtgcgctg gatggcacca
     4201 atcctctcct tcaccgctga tgaagtgtgg ggctatctgc cgggcgaacg tgaaaaatac
     4261 gtcttcaccg gtgagtggta cgaaggcctg tttggtctgg cagacagtga agcaatgaac
     4321 gatgcgttct gggacgagct gttgaaagtg cgtggcgaag tgaacaaagt cattgagcaa
     4381 gcgcgtgccg acaagaaagt gggtggctcg ctggaagcgg cagtaacctt gtatgcagaa
     4441 ccggaactgg cggcgaaact gaccgcgctg ggcgatgaat tacgatttgt cctgttgacc
     4501 tccggcgcta ccgttgcaga ctataacgac gcacctgctg atgctcagca gagcgaagta
     4561 ctcaaagggc tgaaagtcgc gttgagtaaa gccgaaggtg agaagtgccc acgctgctgg
     4621 cactacaccc aggatgtcgg caaggtggcg gaacacgcag aaatctgcgg ccgctgtgtc
     4681 agcaacgtcg ccggtgacgg tgaaaaacgt aagtttgcct gatgagtcaa tcgatctgtt
     4741 caacagggct acgctggctg tggctggtgg tagtcgtgct gattatcgat ctgggcagca
     4801 aatacctgat cctccagaac tttgctctgg gggatacggt cccgctgttc ccgtcgctta
     4861 atctgcatta tgcgcgtaac tatggcgcgg cgtttagttt ccttgccgat agcggcggct
     4921 ggcagcgttg gttctttgcc ggtattgcga ttggtattag cgtgctcctg gcagtgatga
     4981 tgtatcgctc gaaggccacg cagaagctaa acaatatcgc ttacgcgctg attattggcg
     5041 gcgcgctggg caacctgttc gaccgcctgt ggcacggctt cgttgtcgat atgatcgact
     5101 tctacgtcgg cgactggcac ttcgccacct tcaaccttgc cgatactgcc atttgtgtcg
     5161 gtgcggcact gatcgtgctg gaagggtttt taccttccaa agcgaaaaaa caataataaa
     5221 tcctgccgga tgcggcctac agattgctac gaaatcgtag gccgcatccg gcaaaaatcc
     5281 tcaaaaataa gagcaaacct gcatgtctga atctgtacag agcaatagcg ccgtcctggt
     5341 gcacttcacg ctaaaactcg acgatggcac caccgccgag tctactcgca acaacggtaa
     5401 accggcgctg ttccgcctgg gcgatgcttc tctttctgaa gggctggagc aacacctgct
     5461 ggggctgaaa gtgggcgaca aaaccacctt ctcgctggag ccagatgcgg cgtttggcgt
     5521 gccgtcaccg gacctgattc agtacttctc ccgccgtgaa tttatggatg caggcgagcc
     5581 agaaattggc gcaatcatgc tttttaccgc aatggatggc agtgagatgc ctggtgtgat
     5641 ccgcgaaatt aacggcgact ccattaccgt tgatttcaac catccgctgg ccgggcagac
     5701 cgttcatttt gatattgaag tgctggaaat cgatccggca ctggaggcgt aacatgcaga
     5761 tcctgttggc caacccgcgt ggtttttgtg ccggggtaga ccgcgctatc agcattgttg
     5821 aaaacgcgct ggccatttac ggcgcaccga tatatgtccg tcacgaagtg gtgcataacc
     5881 gctacgtggt cgatagcttg cgcgagcgtg gggctatctt tattgagcag attagcgaag
     5941 tgccggacgg cgcgatcctg attttctccg cacacggtgt ttctcaggcg gttcgtaacg
     6001 aagcgaaaag ccgcgatttg acggtgtttg atgccacctg tccgctggtg accaaagtgc
     6061 atatggaagt tgcccgcgcc agtcgccgtg gcgaagaatc tattctcatc ggccacgccg
     6121 ggcacccgga agtggaaggg acgatggggc agtacagcaa ccccgaaggg ggaatgtatc
     6181 tagtcgaatc gccagacgat gtgtggaaac tgacggtcaa aaacgaagag aagctctcct
     6241 ttatgaccca aaccacgctg tcggtggatg acacgtctga tgtgatcgac gcgctgcgta
     6301 aacgcttccc gaaaattgtc ggtccgcgca aagatgacat ctgctacgcc acgactaacc
     6361 gtcaggaagc ggtacgcgcc ctggcagaac aggcggaagt tgtgttggtg gtcggttcga
     6421 aaaactcctc caactccaac cgtctggcgg agctggccca gcgtatgggc aaacacgcgt
     6481 ttttgattga cgatgcgaaa gatatccagg aagagtgggt gaaagaggtt aaatgcgtcg
     6541 gcgtgactgc gggcgcatcg gctccggata ttctggtgca gaatgtggtg gcacgtttgc
     6601 agcagttggg tggtggtgaa gccattccgc tggaaggccg tgaagaaaat attgttttcg
     6661 aagtgccgaa agagctgcgt gtcgatattc gtgaagtcga ttaagtcatt agcagcctaa
     6721 gttatgcgaa aatgccggtc ttgttaccgg cattttttat gaagaaaaca tgcgtttacc
     6781 tatcttcctc gatactgacc ccggcattga cgatgccgtc gccattgccg ccgcgatttt
     6841 tgcccccgaa ctcgacctgc aactgatgac caccgtcgcg ggtaatgtct cggttgagaa
     6901 aactacccgc aatgccctgc aactgctgca tttctggaat gcggagattc cgctcgccca
     6961 gggagccgct gtgccgctgg tacgcgcacc gcgtgatgca gcatctgtgc acggcgaatc
     7021 gggaatggct ggctacgact ttgttgagca caaccgaaag ccactcggga taccggcgtt
     7081 tctggcgatt cgggatgccc tgatgcgtgc accagagccc gttaccctgg tggccatcgg
     7141 cccgttaacc aatattgcgc tgttactttt acaatgcccg gaatgtaaac cgtatattcg
     7201 tcgtctggtg atcatgggcg gttctgctgg tcgcggcaac tgtacaccaa atgccgagtt
     7261 taatatcgct gccgatccag aagcggctgc ctgtgtcttc cgtagcggca tcgaaattgt
     7321 catgtgcggt ctggatgtca ccaatcaggc aatattaacc cctgactatc tcgctacttt
     7381 accggagttg aatcgcaccg gaaaaatgct ccacgccctg tttagccact accgtagcgg
     7441 cagtatgcaa agcggtctgc gaatgcacga tctctgcgcc atcgcctggc tggtgcgccc
     7501 ggacttattc accctcaagc cgtgttttgt cgcggtagaa actcaaggcg aatttacctc
     7561 aggcacgacg gtggttgata tcgacggttg cctgggcaaa ccagccaatg tacaggtggc
     7621 attggatctg aatgtaaaag gcttccaaca gtgggtggct gaggtgctgg ctctggtgcc
     7681 gtaacctgtc acatgttatc ggcatgcagt cattcatcga ctcatgcctt tcactgatat
     7741 ccctccctgt ttatcattaa tttctaatta tcagcgtttt tggctggcgg cgtagcgatg
     7801 cgctggttac tctgaaaacg gtctatgcaa attaacaaaa gagaatagct atgcatgatg
     7861 caaacatccg cgttgccatc gcgggagccg gggggcgtat gggccgccag ttgattcagg
     7921 cggcactggc attagaaggc gtgcagctgg gcgcggcgct ggagcgcgaa ggatcttctt
     7981 tactgggcag cgacgccggt gagctggctg gtgcagggaa aacaggcgtt accgtgcaaa
     8041 gcagtcttga tgcgataaaa gatgattttg atgtgtttat cgattttacc cgtccggaag
     8101 gtacgctgaa ccatctcgct ttttgtcggc agcatggcaa agggatggtg atcggcacta
     8161 cggggtttga cgaagccggt aaacaggcaa ttcgtgacgc cgccgccgat attgcgattg
     8221 tctttgccgc caattttagc gttggcgtta acgtcatgct taagctgctg gagaaagcag
     8281 ccaaagtgat gggtgactac accgatatcg aaattattga agcacatcat agacataaag
     8341 ttgatgcgcc gtcaggcacc gcactggcaa tgggagaggc gatcgcccac gcccttgata
     8401 aagatctgaa agattgcgcg gtctacagtc gtgaaggcca taccggtgaa cgtgtgccag
     8461 gtaccattgg ttttgctacc gtgcgtgcag gtgacatcgt tggcgaacat accgcgatgt
     8521 ttgccgatat tggcgagcgt ctggagatca cccataaggc gtccagccgc atgacatttg
     8581 ctaacggtgc ggtaagatcg gctttgtggt tgagtggtaa ggaaagtggt ctttttgata
     8641 tgcgagatgt gcttgatctc aata
//
LOCUS       DAZPCS010000150         8643 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZPCS010000150 DAZPCS010000000
VERSION     DAZPCS010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8643
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.151"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(355..561)
                     /locus_tag="ACHUQ7_003305"
     CDS             complement(355..561)
                     /locus_tag="ACHUQ7_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFR1136587.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            874..963
                     /gene="kdpF"
                     /locus_tag="ACHUQ7_003306"
     CDS             874..963
                     /gene="kdpF"
                     /locus_tag="ACHUQ7_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFR1136588.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            963..2636
                     /gene="kdpA"
                     /locus_tag="ACHUQ7_003307"
     CDS             963..2636
                     /gene="kdpA"
                     /locus_tag="ACHUQ7_003307"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFR1136589.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGAQQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPSP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2659..4409
                     /gene="kdpB"
                     /locus_tag="ACHUQ7_003308"
                     /pseudo
     CDS             2659..4409
                     /gene="kdpB"
                     /locus_tag="ACHUQ7_003308"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            4715..5287
                     /gene="kdpC"
                     /locus_tag="ACHUQ7_003309"
     CDS             4715..5287
                     /gene="kdpC"
                     /locus_tag="ACHUQ7_003309"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFR1136590.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5280..7960
                     /gene="kdpD"
                     /locus_tag="ACHUQ7_003310"
                     /pseudo
     CDS             5280..7960
                     /gene="kdpD"
                     /locus_tag="ACHUQ7_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
     gene            7957..8642
                     /gene="kdpE"
                     /locus_tag="ACHUQ7_003311"
                     /pseudo
     CDS             7957..8642
                     /gene="kdpE"
                     /locus_tag="ACHUQ7_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
BASE COUNT         1841 a         2233 c         2497 g         2072 t
ORIGIN      
        1 atctgttttt attgcggtag cgatactaat aacaaaattc ggaatttatt tagatgctgg
       61 aattagaaaa agggatgacg gctttctcac cttaccctga agtttcacga aagtgtgtaa
      121 ggaagtatgc aatctaactg gagatttctg atgatctagt cgcttagagc gacaatgaag
      181 gtgaccgcat cctgggtagc cctatgaaag gatactattg aaacgtgatg ctaaaacagt
      241 gatgaaatga ataatacgat ggaaacgtct tccatgttaa ttatcatgga agacgttgat
      301 gttgaacacc taaaccagcc ccctgatacg aggctggctt aagcgcgcag gcaatcagta
      361 aaaatcaccg gttgcctttt cggcctgatc catccacacc ggtttatcgc tggttttcga
      421 ccagacgcgg tgcaggtagc tgtaaaagcg ggcgcggtct ttccagaaaa gcatgaaagg
      481 cagcgccaga acgcccgctg caaccgcata tgtacggcgt aaaaagataa gccatgcagg
      541 atattctctg tagagttcca tacttttctc ccccttttgt atctacccgg tgaatagcac
      601 cggaaaaatg aatttgttta tctgatgaaa atagtaccgc cttttgtgta attttactac
      661 tcatccgacc acttattttt gcttattgat ggtttattta cattcacact gtaattaaat
      721 tacacaaaag ttaaattaat actaaccatc agttaaatca tggcctttgc catttttata
      781 ctttttttac accccgtccg cagatttttg cgaaatcttt gcagccagaa ttctactctt
      841 ccggtatcac ttttaggcca ctggaggtgc actgtgagtg caggcgtgat agccggcgtg
      901 ttgctggtgt ttttattact gggctatttg gtttatgccc tgatcaatgc ggaggcgttc
      961 tgatggctgc gcaagggttc ttactgattg ccacgttttt actggtatta atggtgctgg
     1021 cgcgtccttt aggcagcggg ctggcgcggc tgattaatga cattcctctt ccaggtacag
     1081 cgggcgttga acgtatactc tttcgtttac ctggcgtctc tgaccatgag atgaactgga
     1141 agcaatatct ttgtgccatt ctcggcctga acatgctggg gctggcggtg ctgtttttta
     1201 tgttgctcgg tcagcactat ctgccgctta atccacagca gttgccgggg ctgtcgtggg
     1261 atctggcgct gaataccgcc gtcagctttg tcacgaatac caactggcaa tcttatagcg
     1321 gtgaaaccac gttgagctat ttcagccaga tggcaggctt aacggtacaa aactttcttt
     1381 ctgccgccag cgggattgcg gtgatttttg ccttcatccg tgcgtttact cgccagagca
     1441 tgagcacact cgggaatgcc tgggtcgatc tgctacgcat cacgttatgg gtgctagtcc
     1501 ctgtggcgtt gttgatcgca ctgtttttta ttcaacaagg tgcgcagcaa aactttctgc
     1561 cctatcaggc agtgaatacc gttgaaggag cgcaacagct gttacccatg ggaccagtag
     1621 cttctcagga agcgatcaag atgctcggta ctaacggcgg tggcttcttt aatgccaact
     1681 cgtcgcatcc gtttgaaaac ccaaccgcac tgaccaactt cgtgcagatg ctggcgatct
     1741 tcttgatccc aacagcgctg tgctttgcct ttggtgaagt gacgggcgat cgccgccagg
     1801 ggcgcatgtt gctgtgggcg atgtcagtga tttttgtcat ctgcgtaggc gtggttatgt
     1861 gggcagaagt tcagggtaat cctcatctgc tggcgctcgg cgcagacagc agcatcaata
     1921 tggaaggtaa agagagccgt ttcggcgtgc tggtcagtag cctgtttgcg gtcgtgacta
     1981 cggcggcgtc ctgtggcgcg gtgattgcga tgcatgattc gtttaccgct ctcggcggca
     2041 tggtgccgat gtggctaatg caaattggtg aagtggtgtt cggcggtgtc ggttctggtc
     2101 tttacggcat gatgctgttt gtcctgctgg cggtgtttat tgccgggctg atgattggtc
     2161 gtacaccgga atatctgggt aaaaaaatag acgtacgcga gatgaaactg actgcactgg
     2221 cgattctggt tactccaaca ctggtactga tgggcgcggc gttggcgatg atgaccgacg
     2281 ccggacgtag cgccatgctt aaccctagcc cgcatggttt tagcgaagtg ctgtacgccg
     2341 tgtcgtccgc cgctaacaac aacggcagcg cctttgccgg attaagcgcc aactctccgt
     2401 tctggaactg tttactggcg ttctgcatgt ttgtcggtcg cttcggggtg attatcccgg
     2461 tgatggcaat tgccggttcg ctggtgagta aaaagagcca acccgccagc tccggcacgc
     2521 tgccaacgca cggcccgctg tttgttggcc tgttaatcgg caccgtgttg ctggttggcg
     2581 cactgacctt tatccctgcc ctggcgcttg gtccggtggc ggaatatctc tcctgatgat
     2641 attgagtgag cactgaatat gagtcgtaaa caactggcgc tattcgaacc aacacttgtc
     2701 gttcaggcgc tgaaagaagc ggtgaaaaaa ttaaacccgc aggcgcaatg gcgcaatccg
     2761 gtgatgttta tcgtctggat cggcagtctg ctgaccacct gtattagcat cgcgatggca
     2821 agcggtgcga tgcctggcaa tgcgctgttt agcgcggcaa ttagcggctg gctgtgggtc
     2881 accgtactgt tcgctaattt cgccgaggcg ctggcagaag gccgcagtaa agcgcaggcc
     2941 aacagtctta aaggggtgaa aaaaactgcc tttgcccgca agctgcgtga gccgaaatat
     3001 ggcgctgcgg cggacaaagt tcctgccgat caactgcgta aaggtgacat cgtgctggta
     3061 gaagctggcg atattatccc ctgcgatggt gaagtcattg aaggcggcgc atcggtcgat
     3121 gaaagcgcca tcaccgggga atcggcaccg gtgatccgtg aatccggcgg cgattttgcc
     3181 tccgttaccg gcggcacgcg tattctttct gactggctgg tgattgagtg tagcgttaac
     3241 cccggtgaga catttctgga tcggatgatc gcgatggtgg aaggcgcaca gcgacgcaaa
     3301 acaccgaacg agattgccct gactatcctg ctgattgctc tgaccatcgt ctttttactg
     3361 gcaaccgcca cgctgtggcc gttttccgcg tggggcggta atgccgtgag tgtaacggta
     3421 ctggtggcgc tgctggtctg tctgatccca accactattg gtggcctgtt gtcagcgatc
     3481 ggcgtcgccg ggatgagtcg gatgctgggc gcgaatgtga ttgccaccag cggacgtgca
     3541 gttgaagcgg caggtgacgt tgacgttctg ctactggata aaaccggcac catcacactc
     3601 ggtaaccgtc aggcgtcgga gtttatcccc gcgcagggcg tggatgaaaa aacgctggct
     3661 gacgccgcac aactggcttc gctggctgat gaaacgccgg aaggtcgcag tattgtgatc
     3721 ctcgccaagc aacgttttaa cctgcgcgag cgcgatgtgc agtcgctcca tgccaccttt
     3781 gtaccgttta ctgcgcaaag ccggatgagc gggatcaaca tcgacaaccg catgatccgt
     3841 aaaggttctg tcgatgccat tcgccgccat attgaggcta acggcggtca cttccctgcc
     3901 gatgttgatc aaaaagtcga tcaggttgcg cgtcaaggag ccacgctgct ggtggtggtg
     3961 gaaggttctc gtgtgctggg cgttattgcg ctgaaagata tcgtcaaagg cggtattaaa
     4021 gagcgcttcg cccagctgcg caaatgggca ttaaaacggt gatgattacc ggcgataacc
     4081 gtctgactgc cgccgcgata gctgcggaag cgggtgtcga tgattttctc gccgaagcga
     4141 caccggaggc caagctggca ttgatccgtc agtatcaggc ggaaggtcgt ttggtagcga
     4201 tgaccggcga cagcaccaac gatgctacgg cgctggcgca ggcagatgtc gcggtggcga
     4261 tgaactccgg cacccaggcg gctaaagagg cgggcaatat ggtggatctc gactctaacc
     4321 cgaccaagtt gatcgaggtg gtgcacattg gcaaacagat gctgatgacc cgtggctcgc
     4381 tgaccacctt cagcattgcc aacgatgtgg cgaaatactt cgccattatt ccggcggcgt
     4441 tcgcggcaac atatccgcag ttaaatgcgc tgaacatcat gcgcctgcat tcgcccgact
     4501 ccgccattct cagtgcggtc attttcaacg ccttgattat cgtctttttg attcctctgg
     4561 cgttaaaagg cgtgagttat aaaccgctta ccgcttctgc catgttgcgc cgtaacttat
     4621 ggatttacgg tctgggtggg ctgctggtgc cgtttattgg tatcaaagtc attgatttac
     4681 tgctaaccgt ttgtagtctg gtgtgaggtt taccatgagt ggattacgtc cggcattatc
     4741 gacatttctc tttctgttat tgattactgg cggcgtttac ccgctgctga ccaccgcact
     4801 ggggcaatgg tggtatccct ggcaggccaa cggttcgttg attcgtgaag gtgatacggt
     4861 gcgcggttcg gcattaatcg ggcaaaattt taccggcaac ggctattttc atggtcgccc
     4921 gtcggcaaca gcagaaatgc cctataatcc acaggcttct ggcgggagca atctggcagt
     4981 cagtaacccg gagctggata aacagatagc cgcacgcgtt gccgcattac gggccgctaa
     5041 cccggacgcc agcacgaacg ttccggttga actggtgacg gcgtcggcaa gcggactgga
     5101 caataacatc accccgcaag cggcggcctg gcagatccca cgcatcgcaa aagcgcgtaa
     5161 tctcagcgtt gaacagctca cgcagctgat cgcaaaatac agccaacaac cgctggtgaa
     5221 atatatcggc cagccggtcg tcaacattgt cgaactcaat ctggcactgg ataagcttga
     5281 tgaataacga acccttacgt cccgaccccg accgtctgct ggaacaaact gccgcgccgc
     5341 atcgggggaa gctgaaagtt ttcttcagtg cctgtgcagg cgtcgggaag acctgggcga
     5401 tgctggcaga agcccagcga ctgcgggcgc aagggctgga tattgtggtt ggtgtggtag
     5461 aaacccatgg gcggaaagat accgccgcca tgcttgaagg gctggctgtt ctgcctctga
     5521 aacgccaggc gtaccgtggg cggcatatca gcgagtttga tctcgatgcc gccctcgccc
     5581 gccgtccggc gctgatctta atggacgaac tggcgcacag taatgcgcca ggttctcgtc
     5641 atcctaaacg ctggcaggat atcgaagaac tgctggaagc tggcattgat gttttcacaa
     5701 ccgtcaacgt tcagcatctg gaaagtctga atgatgtggt cagcggcgtc accggaattc
     5761 aggtacggga aaccgtgccc gatccttttt tcgatgccgc cgacgacgtg gtgctggtgg
     5821 acttgccccc ggacgatctg cgccagcggc tgaaagaagg caaagtctat attgccgggc
     5881 aggcggagcg cgccattgaa cattttttcc gcaaaggcaa tctgatcgcc ctgcgcgaac
     5941 tggcactgcg ccgtactgcc gatcgcgttg atgagcaaat gcgcgcctgg cgggggcatc
     6001 ctggcgaaga gaaagtctgg cacacgcgcg acgcgatcct tttatgcatc ggccataaca
     6061 ccggcagcga aaactggtcc gcgcggcggc gcggctgacg tcacggctgg ggagcgtctg
     6121 gcacgcggtg tatgttgaaa cccctgccct gcatcgctta ccggagaaaa aacgtcgggc
     6181 aattctcagc gccttacgtc tggcgcagga gctgggcgcg gagacggcaa cactttctga
     6241 tccagcggaa gagaaagcgg tagtgcgtta tgcccgtgaa cataatctcg gcaagattat
     6301 tctcggtcgc ccagcctcgc gccgctggtg gcgtcgggaa acatttgctg accgactggc
     6361 gcgcatcgcc cccgatctcg atcaggtgct ggtcgcgctt gatgaaccac ccgcccgcac
     6421 gattaacaac gcgccggata gccgctcatt taaagacaag tggcgggtac agattcaggg
     6481 atgcgtggtt gccgccgcgt tatgcgccgt tatcacctta attgccatgc agtggctgat
     6541 ggcgtttgat gccgccaacc tggtgatgct gtatctgctt ggcgtggtgg tggcgctatt
     6601 ttatggacgc tggccttcgg tggttgccac cgtcattaat gtggtgagtt tcgatctctt
     6661 ttttatcgcc ccacgcggca cgctcgccgt ctctgatgtg caatatctgc tgaccttcgc
     6721 ggtgatgtta accgtcgggc tggtgatcgg gaaccttact gctggtgtgc gttatcaggc
     6781 gcgggtagcc cgttaccgcg agcaacgcac acggcactta tatgaaatgt cgaaagctct
     6841 ggcggtgggc cgcagtccgc aggacatcgc cgccaccagc gaacaattta ttgcctccac
     6901 gtttcatgcc cgcagtcagg tgttgttgcc cgatgacaac ggtaaattgc agccgttaac
     6961 acatccgcaa ggaattacgc catgggacga tgccatcgcg cagtggagtt ttgataaagg
     7021 cctgcctgcg ggcgcgggca ccgacacgtt acccggtgta ccgtaccaga ttttgccgct
     7081 aaaaagcggc gagaaaacct acgggctggt ggtggtggag ccggggaatc tgcgccagtt
     7141 gatgatcccg gaacagcagc gcctgctgga gacttttacg ctgttagtcg ccaatgccct
     7201 tgagcggctg acgctaaccg ccagcgaaga acaggcgcgg atggcaagcg aacgtgaaca
     7261 gatccgcaac gccctgctgg cagcgctttc gcatgattta cgcacgccgc ttacggtgct
     7321 gtttggtcag gcagaaatct taacgctcga tctggcaagc gaaggatcac cccacgcccg
     7381 ccaggccagc gagattcgtc agcatgtgct gaacactacc cgactggtga ataatctact
     7441 ggatatggcg cgaattcagt ccggcggctt taatttgaag aaagagtggt taacgctgga
     7501 agaagtagtc ggcagcgcgc tgcaaatgcc ggaaccgggt ttatcgtcgc ccatcaatct
     7561 ttctctgcca gaaccgctga ccttaatcca cgttgacggg ccactctttg aacgggtgct
     7621 gattaatctg ctggagaacg cggtgaaata tgcgggtgcg caggccgaaa ttggtatcga
     7681 tgcccacgtt gagggcgaaa atctacaact ggatgtctgg gataacggcc ccggtcttcc
     7741 gccaggccag gagcagacga tatttgataa gtttgctcgc gggaataaag agtcggcggt
     7801 gccgggtgtc gggcttgggt tggcaatttg tcgggcgatt gtggatgtac acggcggcac
     7861 cattaccgcg ttcaaccgac cggaaggtgg tgcctgtttt cgtgttacac ttccccagca
     7921 aaccgctcct gaacttgaag actttcatga ggatatgtga caaacgttct gattgttgaa
     7981 gatgaacagg ctattcgtcg ctttctgcgc acggcgctgg agggcgacgg gatgcgcgtc
     8041 tttgaggccg aaactctgca acgcggcttg ctggaagcgg caacccgtaa gccagatttg
     8101 attattctcg atctcggcct gcccgatggt gatgggattg agtttatccg cgacctgcgc
     8161 cagtggagcc cggtgccggt gattgtgctt tccgcacgca gcgaagagag cgacaagatc
     8221 gccgcgctgg atgccggagc ggatgattat ctgagtaagc cgtttggtat tggcgaattg
     8281 caggcccgtc tgcgcgtcgc tttacgccgc cactccgcca ctccgccacc gccgcgcccg
     8341 atccgctggt gaaattttcc ggtgttaccg tcgatttagc cgcccgcgtg attcaccggg
     8401 gtgaggaaga ggtgcatctc acaccaattg agttccgcct gctggcggtg ctgctcaaca
     8461 atgccggaaa agtactcacc cagcgccagc tccttaacca ggtgtggggg ccaaacgcgg
     8521 tcgaacacag tcactatttg cgtatttata tgggacatct gcgacaaaaa ctggaacagg
     8581 atccggcccg gcctcgccac ttcattactg aaaccggtat tgggtatcgg tttatgcttt
     8641 gaa
//
LOCUS       DAZPCS010000151         8549 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZPCS010000151 DAZPCS010000000
VERSION     DAZPCS010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8549
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.152"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            1..1889
                     /gene="espL4"
                     /locus_tag="ACHUQ7_003312"
                     /pseudo
     CDS             1..1889
                     /gene="espL4"
                     /locus_tag="ACHUQ7_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
     gene            complement(1858..3595)
                     /gene="aceK"
                     /locus_tag="ACHUQ7_003313"
                     /pseudo
     CDS             complement(1858..3595)
                     /gene="aceK"
                     /locus_tag="ACHUQ7_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
     gene            complement(3779..5083)
                     /gene="aceA"
                     /locus_tag="ACHUQ7_003314"
     CDS             complement(3779..5083)
                     /gene="aceA"
                     /locus_tag="ACHUQ7_003314"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HFR1136591.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINNLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSVGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(5113..6714)
                     /gene="aceB"
                     /locus_tag="ACHUQ7_003315"
                     /pseudo
     CDS             complement(5113..6714)
                     /gene="aceB"
                     /locus_tag="ACHUQ7_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            complement(6983..7912)
                     /gene="metA"
                     /locus_tag="ACHUQ7_003316"
     CDS             complement(6983..7912)
                     /gene="metA"
                     /locus_tag="ACHUQ7_003316"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFR1136592.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYLRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            8069..8512
                     /locus_tag="ACHUQ7_003317"
     CDS             8069..8512
                     /locus_tag="ACHUQ7_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFR1136593.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         2199 a         2084 c         2103 g         2163 t
ORIGIN      
        1 tgctacaata tttgaagaat acctaatgga aattttacct caacgctcag acactcttga
       61 taaacaagaa ttaaaccaaa aatcagatag aaaagaaaaa gaattcccaa gaattaaact
      121 taatggtcaa tgttatttcc cggggcgccc caaaaccgta tagtataccg acacattgct
      181 gcacaatata ttaatgatat ttatcagaat gttgattaca aaccccatca agatgattac
      241 tcttcagctg aaaaatttct cactcacttc aacaaaaaat gcaaaaacca gactttggcg
      301 ttgatttcca gccgtcctga ggggcgttgc gttgctgcct gcggtgattt cgggctagtt
      361 atgaaagcat attttgacaa gatggaatca aatggcatca gtgttatggc agccatatta
      421 ctggtggata accatgcttt gacggtccgg ctaagaataa agaacacaac tgaaggatgt
      481 acccattacg tggtttcggt ttatgatcct aatgtaacta acgataaaat aagaattatg
      541 agcgaaagca aagaggatat taaacactat tctctgatgg attttatgaa tgtagattat
      601 agcctcctga aatggtcaaa tgatcatgtt attaaccaat ctgttgcaat aattccagca
      661 cttccgaaag aacagctatt gatgttaaaa ggaactgtgg atgaaataac ccctccatta
      721 tcaccagcaa cgatgaattt gctaatggca attggtcaga atcaccaact tacgcaactg
      781 atgattcagc tccagaaaat gccagaacta catagaacag aaatgttgac tgcctataat
      841 agtattaacc tccccggttt atatttggct ataaattatg gcaatgcgga tatcgttgag
      901 actattttca attcattgtc agaaacagga tatgaagggt tactctcgaa aaaaaatctc
      961 atgcatattc tggaggcaaa agataaaaat ggtttttctg gattattttt agcgatatca
     1021 cgtaaggata aaaatgttgt aacctcgatt ctgaacgtct tacctaaact ggccgcaaca
     1081 catcatttag ataatgaaca ggtgtataaa ttcctgagtg ccaaaaatag aacgtccagc
     1141 catgttttat accatgttat ggcgaatggt gatgccgaca tgctgaaaat ttttttggtc
     1201 gcgttacctt tgttaattcg cacatgtcat ttgactaaag aacaggtact cgatctcctg
     1261 aaggcaaagg atttttatgg ttgcccaaga ctatacctgg cgatgcaaaa tggacatagc
     1321 gatatcgtga aagttattct cgaagcattg ccctgtctgg cccaggaaat taacatttca
     1381 gcttccgata ttgtagatct tctgaccgct aaaagtcttg cgcgcgacac gggtttgttt
     1441 atggccatgc agcgcggaca tatgaacgtt attaatacta tttttaacgc attacccact
     1501 ctttttaata cgtttaaatt cgataaaaaa atatgaagcc cctcctcctg gcaaataatt
     1561 ctaatgaata tcccggtttg ttttcagcga tacagcataa acaacaaaac gttgtagaga
     1621 cggtttatct tgctttatct gaccatgcac gcctgtttgg atttaccgct gaagatatta
     1681 tggatttttg gcaacacaaa gcgccacaaa aatactctgc ctttgagttg gcttttgaat
     1741 ttggtcaccg ggttattgct gaattaatcc ttaatacatt aaataagatg gctgaaagct
     1801 ttggctttac ggataaccct cgatacattg cggagaaaaa ttatatggaa gctttactca
     1861 aaaaagcatc tccccatacc gtacgctaaa tctttgcctg cgccgatacg cataaacatc
     1921 ttccacatgc ccttcacgta tgcggttttg tagtgcgcgc cagtaatcag cgcggaacag
     1981 gtcggcgtgc atctcttcaa acagcggacc aatacgcggg tcggcgcata gccagtggcg
     2041 aaactcttcc gggaaaacat cgcccggcga gacgctgtac cacggttcgc tggcaagttc
     2101 gtcttccgga tagcgcggcg gcgggatgtc gcggaaattc acttccgtca tgtagcaaat
     2161 ttcatcgtaa tcataaaaaa ccacacgccc gtgacgggtg acaccgaagt ttttaaacag
     2221 catgtcgcca gggaaaatgt tagcagcggc aagctggcga atagcgttac cgtattcttc
     2281 aatggcgtcg cgcaactgct gaccttccac ttgttccagc cagatattga gcggcaccat
     2341 ccgccgctcg attatagaga tggcgaatta caatctgctc accaagatcg gtgatttttt
     2401 ccgctgcttc ctggagcagt aattccatta ataccgggga aatatgccgc ttctccagca
     2461 caaagttttc aaactcctgg gtgtccgcca ttcggcccac acggtcgtgc tctttcacca
     2521 gttgatagca ggcacgaacg tgagcggcag acatctcttt ctgcggcgcg aacttgtctt
     2581 tgatgacttt gaatacccga tcaaagcccg gcagcgtaaa caccaacatc accattccac
     2641 gaatacccgg cgcttcaatg aactgctcat tacagccctg tagataaacg agatattcgc
     2701 ggtagctttc ggttttggcg tgcttctggc agccgatagc catatacaat tcagcagtgg
     2761 ttttacctgg cagaatttcc cgtagccact cgaccagcgc tgcgggcagc ggtgcataaa
     2821 ccataaaata agaacgcgca aagccaaaaa caatgctcgc ttcggcggtc gtcgtcaggc
     2881 aggtatcaat aaataactcg ccgtcgtccg tctggtggat cggcagtaag aagggcaacg
     2941 tgccggaagg cgtgatcagt ttgcccacca gccaggcggc tttattgcgg taaaacagtt
     3001 cgttcgccac ctgtaaatga ctttccgcga ggttgtctgt ccccagcgtt tccgtcagat
     3061 ggcgaatgat gtaatgaatg tcacggcttt tattctgcca gcgcaggcgc agcggcaggt
     3121 cgctgataac gcgcatcagt agagattccc agccgtgatc ggggtgaaag tctttcgcca
     3181 gcgggcgggg aatggtacga aagcggcgct ctggctgaga gctaaagata aaaagccgct
     3241 cgggagtaag cgagcggtgg tcaaataacc gacagtacac ggagttaaaa aagctctccg
     3301 caatctcgaa gcgcgggtaa tccggcaaca gccgcgtgta atgctcttta acgcgcagta
     3361 aaaatgccgc gtccgtactt tggccgttag taatgcagcg cagttgctcc acgaccagac
     3421 caacgtgatg atcgtaaaga tggatacggt ttttcatcgc ctgctggaca gcatgccagt
     3481 cggcctgttc gaaacgctgc tgcgccccgg aagtcacttc gaggaatcga ccatactgag
     3541 cgtcgaagcc ttgcaaaatg gtttgagcaa tcagtaattc caggccacgc ggcatttagc
     3601 gccctcatca ggagcagaga attgccggat gcggcgtaaa cgccttatcc agcctacgtt
     3661 cggcaacggt tgtaggcatg ataagacgcg caagcgtcgc atcaggcatc gtgcaccgat
     3721 tgccggatgc ggcgtaaatg ccttatccgg cctacagtca gcaacgtttg taggttgctt
     3781 agaactgcga ttcttcagtg gagccagtca gcgcggtgac tgaagacgtg ccgccctgaa
     3841 taatagtcgt cactttatcg aagtaacctg tacccacttc ctgctggtga gatacgaagg
     3901 tatagccatc tttcgcggcg gcaaattccg gctgctgcac tttctcaaca tagtgcttca
     3961 taccttcgcc ctgcgcataa gcgtttgcca ggtcgaacat gttaaaccac atgctgtgga
     4021 tacctgctag ggtgatgaac tggaacttgt agcccatatc cgacagctgc tgctggaagc
     4081 tggcaatagt tttgtcgtcg aggtttttct gccagttgaa cgacggcgag cagttataag
     4141 ccagcagttt gcccggatat ttcgcgtgga tagcttgtgc aaagcgacgc gccagttcca
     4201 gatccggcgt ggaggtttca caccagacca ggtcagcata tggcgcatac gccaggccac
     4261 ggctgatcgc ttgctcaatg cccgcatgag tacggaagaa gccttcactg gtacgctcgc
     4321 cggtaataaa ttcgctgtca tacgggtcgc aatctgaggt gatcagatcc gccgcatcag
     4381 catcggtacg tgcaaccagc agggttggaa cgcccgtcac gtcagctgcc agacgcgccg
     4441 cgaccagttt ctgaatagct tcctgagttg gcactaaaac tttgccgccc atgtgaccgc
     4501 atttcttcac tgacgccagc tgatcttcga agtgaactgc cgcagcaccg gcttcaatca
     4561 tcgctttcat cagttcaaag gcattcagga caccgccaaa accggcttcc gcatcggcaa
     4621 cgatcggcag gaagtaatcg acatagcgcg gatcgcccgg ctcaatgccc acggaccatt
     4681 ggatctgatc ggcacgacgg aaggtgttgt tgatccgctc caccacagct ggcaccgagt
     4741 ttgccggata gagcgactga tccggataca tgctggccgc caggttagcg tccgccgcta
     4801 cctgccatcc cgacagatag actgcttcaa tacccgcttt cgcctgttgc agcgcctgac
     4861 cgccagtcag tgcaccgagg ttgttgatgt agcctttttt cgactcaccg tgcagcagac
     4921 gccacatttt cgctgcgccc agttgcgcca gcgtgcattc aggattgact gaaccgcgta
     4981 atttcaccac atcttccgca ctgtatgggc gagtaatgcc ttcccaacgc ggttgagtcc
     5041 actctttctg taattcttca atttgttgtg tacgggtttt catgtgcaga tgctccatag
     5101 ttatgtggtg gtttacgcta acaggcggta gcctggcagg gtcaggaaat caattaactc
     5161 atcggaagtg gtgatctgtt ccatcaagcg tgcggcatcg tcaaaacggc cctgggagaa
     5221 acgttcttcg cccagttcgc tggcaatgac tttcatctct tcgcccagca tctggcggaa
     5281 caaggctttg gtcaccggtt tgccattgct caacgttttt tgatgatgga tccactgcca
     5341 gatcgaggta cgggaaattt cagccgtcgc cgcatcttcc atcaggccat aaatcggcac
     5401 gcagccgttg ccggagatcc acgcttcgat gtactgcaca gccacgcgaa tgttagcgcg
     5461 cataccttct tcggtgcgtt caccatcaca aggtgccagc agctgatcgg cagtaatcgg
     5521 cgcgtcttgt tcgcgcaata cttcaagctg atttttacgg gagccgagaa tgtcgttgaa
     5581 taccgccatt gccgtgtccg caaggcctgg gtgagcgatc catgtgccat cgtgaccgtt
     5641 attggcttcc agcgctttat ccgcttttac tttgttgagc acctggttat tacgctcttc
     5701 atctttgctc ggaataaacg cggccatgcc gcccatcgca aaagcaccgc gtttatggca
     5761 ggttttaatc agcaggcgtg agtaagcatt caggaatggt ttatccatcg tcacggcctg
     5821 tctgtctggc aggacacgat cgggatagtt tttcaacgtt ttgatatagc tgaagatgta
     5881 atcccagcga ccgcagttca gaccaacaat atggtcgcgc agcgcgtgaa ggatttcgtc
     5941 catctggaac acggcgggca gcgtttcaat cagcaacgtc gccttgatgg tgccgcgcgg
     6001 cagattaaag cgatcttctg catagctgaa gacttcgctc caccaggccg cttcctgcta
     6061 ggactgggtt ttcggcagat agaaataggg accgctgccc tttgccaaca gtgcctgata
     6121 gttgtggaag aaatagagcg caaaatcaaa caggctaccg gggattgcct caccacgcca
     6181 ggtgacatgt ttttccggca agtgcagacc gcgtacccga caaatcaaaa ccgctggatt
     6241 gggcttgagc tgataaattt tgcctgcttc attggtatag ctgatggtac cgttaaccgc
     6301 atcgcgcagg ttaatttgcc cgtcgatcac tttgttccag tctggtgcca gtgaatcttc
     6361 gaaatcggcc ataaagactt tcacattggc gttcagtgcg ttgatcacca tcttgcgctc
     6421 taccgggcca gttatctcta cgcggcggtc ttctaagtcc gcaggaatcc cgcgaatttt
     6481 ccaatcagca tcgcgaatgg aagccgtttc cgaaataaaa tcaggcaacg ttccgttatc
     6541 aatatcttgc tgctgctgaa tgcgcgctgc cagaagttta ttgcgttgtg gcgtaaaatg
     6601 cgtcaccagc tcagtcagaa attctaccgc ttcggcagta agaatttgct tctcctgctc
     6661 gccatacggc cttgtgaaag ccagttcatc ggttgttgtt gcctgttcag tcatcgtgca
     6721 gctcctcgtc atggatccga aaacttcccc tctgtgaacg aaggaacgtt gtgcgctttt
     6781 cgttcagcac aactaagact actcatttaa aatgcaaaat caaaaacaat ttccatttta
     6841 attaaaaata cttgataact tattgataac aatatgaata aaatttattc acttgctgag
     6901 gtgcgtttcg gaaataaaaa aggcacccga aggtgcctga ggtaaggtgc tgaatcgctt
     6961 aacgatcgac tatcacagaa gattaatcca gcgttggatt catgtgccgt agatcgtatg
     7021 gcgtgatctg atagacgtaa tagttgagcc agttggtaaa cagcaaatta ccgtgactac
     7081 gccagctcgc tcgcggtttg ttttgcggat cgttgtgcgg gaaatagtta tacggtacat
     7141 ccgggtctag tccggcttcc acatcgcgta aatattcctg cgccagcgtt tgcgcatcat
     7201 attcgggatg gcccgtcaca aaggcaatgc gcttatcttt gctggcaaac agatatgcat
     7261 ccccttcttc cgtctctgcc agaatttcca gatcggtgta atcacgaatc aacgctgccg
     7321 gaaagtcagc atagcgcgaa tgcggtgcca ggaatgaatc atcaaagcca cgcgtcagaa
     7381 gcgcatgagg atggagaata tgatgctcgt aaacgccaga gagtttgtcg gtgcgagttt
     7441 gcttaggaat gccgtagagg atattgagcg cggcctgtac cgcccagcag acaaacagcg
     7501 tcgaggtgac gtgatctttc gaccactcca gcacctgttt gatctgcggc cagtaagcga
     7561 catcattaaa ctccaccagg cccagcggcg caccggttac aatcagaccg tcaaagttct
     7621 gttcctgaat atcttcaaag ttacagtaga agttgttcag atgctctgcg ggcgtgttgc
     7681 gcgattcacg ggaatcgatg cgcaacagct gaatatcgac ctgcaaaggt gagtttgaaa
     7741 gcaggcgcag aaactgattt tcagtttcaa tcttcttcgg catcaggtta aggatcagaa
     7801 ccttaagtgg acgaatttcc tgaccagacg cacgagaagt tgtcatcaca aagacgtttt
     7861 cttcacgcaa gaaattgacg gcgggtagct cgtccggcac acgaatcggc ataacctgat
     7921 tacctcacta catacgctta tacgtttaga catccagata gctgaagata accagaaaat
     7981 ttgccaatgt cgagcctgca tgttgaaggt gagaaagttt caaggaaaag gctacgttag
     8041 aatataagaa tgacgaaaag gagagaaaat ggttattagt attcgccgct cacggcatga
     8101 ggaaggggag gaactcgttg cgatttggtg tcgatctgtc gatgccaccc acgatttttt
     8161 atcagcagag tatcgggccg agctggaaga gctggtgcgt tcctttctgc cggaagcacc
     8221 gttgtgggtc gcggttaatg aacgggagca gccggttggg tttatgttgt taagtgggca
     8281 gcatatggat gcgctgttta tcgatcctga tgtgtgcggc tgcggcgtgg gtcggatgct
     8341 ggtggagcat gcgctctcga tggcaccgga actgacaacc aacgttaatg agcaaaatga
     8401 gcaggcggtt gggttctata agaaggtggg ttttaaggtt acgggacgct ctgaggtgga
     8461 cgatttgggg aaaccgtatc cgttgctgaa tctggcgtat gtgggggaat aagccgcctc
     8521 tgccgtgcct gcccaagctg gagaagacg
//
LOCUS       DAZPCS010000152         8528 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZPCS010000152 DAZPCS010000000
VERSION     DAZPCS010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8528
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.153"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..189
                     /gene="csgC"
                     /locus_tag="ACHUQ7_003318"
     CDS             <1..189
                     /gene="csgC"
                     /locus_tag="ACHUQ7_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFR1136594.1"
                     /translation="GSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVS
                     DGQALHLSQQWTPSSEKS"
     gene            310..621
                     /gene="ymdA"
                     /locus_tag="ACHUQ7_003319"
     CDS             310..621
                     /gene="ymdA"
                     /locus_tag="ACHUQ7_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFR1136595.1"
                     /translation="MFRPFLDSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSIPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            716..1249
                     /gene="ymdB"
                     /locus_tag="ACHUQ7_003320"
     CDS             716..1249
                     /gene="ymdB"
                     /locus_tag="ACHUQ7_003320"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFR1136596.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYSYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            1191..2672
                     /gene="clsC"
                     /locus_tag="ACHUQ7_003321"
     CDS             1191..2672
                     /gene="clsC"
                     /locus_tag="ACHUQ7_003321"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFR1136597.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEVILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(2680..3837)
                     /gene="mdoC"
                     /locus_tag="ACHUQ7_003322"
     CDS             complement(2680..3837)
                     /gene="mdoC"
                     /locus_tag="ACHUQ7_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFR1136598.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            4231..5766
                     /gene="mdoG"
                     /locus_tag="ACHUQ7_003323"
     CDS             4231..5766
                     /gene="mdoG"
                     /locus_tag="ACHUQ7_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFR1136599.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPRGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKITEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            5759..8302
                     /gene="mdoH"
                     /locus_tag="ACHUQ7_003324"
     CDS             5759..8302
                     /gene="mdoH"
                     /locus_tag="ACHUQ7_003324"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFR1136600.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
BASE COUNT         2150 a         2025 c         2222 g         2131 t
ORIGIN      
        1 ggcagttcag ggcaaagtca gacgaagcaa gaaaagaccc tctcattgcc tgctaatcaa
       61 cccattgctt tgacgaagtt gagtttaaat atttccccgg aagatcgggt gaaaatagtt
      121 gttactgttt ctgatggaca ggcacttcat ttatcacaac aatggacgcc ctcttcagaa
      181 aagtcttaat ttgttgaaat atcgagcata agatgaatct ggagagaatg gtctgctgcg
      241 aatcagccaa cctgaaagta tggataacac aaccttcaag gatgactaat cattgaggaa
      301 atagaataaa tgttcagacc ttttttagac tctcttatgc tcggcagttt gttttttcct
      361 tttattgcca ttgcaggaag caccgcgcaa gggggcgtga tccattttta tggccaaatt
      421 gtggaaccgg catgtgacgt cagcacccag tcatcacccg tagaaatgaa ctgcccacag
      481 aatggttcta ttccgggcaa aacctactcc agcaaagcgt taatgagcgg caatgtcaaa
      541 aacgcgcaaa tagcctcagt aaaagtgcag tatcttgata aacagaaaaa gctggctgta
      601 atgaacatcg aatataacta agtttctgaa caactcacgt tgctgagcag aaaaatgcga
      661 tttaaccaaa aagcctgctg tacacttaag aaacaagaag gtgtaaggag gcgttatgaa
      721 aacgcgtatt catgttgtgc agggtgatat taccaaactg gccgttgatg tgattgtgaa
      781 tgcggctaat ccgtcattaa tgggaggcgg cggcgtcgat ggggccattc atcgcgcagc
      841 gggtccggcc ctgctggatg cctgtttaaa agtcaggcaa cagcagggcg attgccctac
      901 ggggcatgcc gttattacgc ttgcaggcga tcttcccgct aaagccgtag tacacaccgt
      961 cgggccagtc tggcgtggtg gggaacaaaa cgaagaccag cttttgcagg atgcctatct
     1021 gaatagccta cgactggtgg cggcaaacag ctatacgtca gtggcttttc ctgcaatcag
     1081 tactggggtt tatagttacc ctcgtgcggc agcggctgaa atcgcagtta aaaccgtttc
     1141 agaatttatt acccgtcacg ctttacccga acaggtatac tttgtctgtt atgatgaaga
     1201 aaacgcccac ctctacgaaa gactccttac ccaacaagga gatgaatgat ttgccccggc
     1261 tggcgagcgc ggtgctgcca ctgtgttcgc aacatcccgg tcagtgtggt cttttccctc
     1321 tggagaaaag tctggatgcg tttgccgccc ggtatcgtct ggccgaaatg gcagagcata
     1381 cgctggatgt tcagtattac atctggcagg acgatatgtc ggggcggtta ctgttttccg
     1441 ccctgttagc cgcagcaaag cgtggcgttc gcgtccgttt gttgctggac gacaacaata
     1501 cgcccggact tgacgacatt ttgcgcttgc ttgacagtca tccacgcatt gaagtccggc
     1561 tttttaatcc tttctcgttt cgcttgctac gtccgcttgg atatatcacc gacttttccc
     1621 gtctcaatcg ccggatgcac aataaaagtt tcactgtcga tggcgtggtt accctagtgg
     1681 gaggacgaaa tattggtgat gcctattttg gtgcagggga agagccactt ttttcggatt
     1741 tagatgtcat ggcaataggc cccgtggtag aggatgttgc cgatgatttc gcccgctact
     1801 ggtattgcaa atcggtttca cccttacagc aggtgctgga tgtcccggaa ggtgaaatgg
     1861 cggatcgcat cgagttaccc gcctcctggc ataacgatgc catgacgcat cgttatttac
     1921 gcaaaatgga atccagtcca tttataaatc atctggttga tggaacgttg ccgcttatct
     1981 gggcgaagac acgtttatta agtgatgatc cggcgaaagg ggagggcaag gcaaaacggc
     2041 attcactgtt accgcagcgc ctgttcgata tcatgggctc acccagtgaa cgcatcgata
     2101 ttatctcttc ctattttgta ccgacacgcg caggtgtggc gcaactctta cggatggtga
     2161 gaaaaggggt aaagattgcg atcctaacca attctcttgc cgctaacgat gttgctgtcg
     2221 tccatgccgg atacgcgcgc tggcgcaaaa aattgctccg ctatggcgtg gaattatatg
     2281 aactcaagcc gacgcgtgaa caaagcagta cgttacacga tcgcggcata accggtaatt
     2341 ccggtgccag cctgcatgct aaaactttta gcatcgatgg taaaacggtg tttatcggct
     2401 ctttcaattt cgatccgcgt tcaacattgc tcaatactga aatgggcttc gtgatagaga
     2461 gcgaaacgct ggcacagtta attgataaac gctttattca gagccagtac gatgcggcct
     2521 ggcagctccg tctggacagg tggggacgga tcaactgggt tgatcgtcat gcaaagaaag
     2581 aggttattct caaaaaagaa cccgccacca gtttctggaa gcgggttatg gtcagactgg
     2641 cgtcgatatt gcccgtggaa tggttattgt aaatgtggct taacgggctg gtgctttatc
     2701 gttctcacgc ttaacaaccg gtttaccaga gaacaaaaac ttcagtaacg ggatgcgtaa
     2761 atgaatttca tacagaatta tcgcaatccc tactacgaat atcaggccac agagaaaacc
     2821 aagccagttg gaggtgatgt gcggtgtaat gtatgcgccg aaaaacagcg ttaacgggtg
     2881 gtgaaccaga tagataaaca gcgatgcgtt aacaaaataa gtcacccgcg ctgactggaa
     2941 gttaagcaaa cggtggccaa aggagaagac cacattcacc atccacaggc cgaggaccat
     3001 ggtgatcacc gactcggttt cgtacatcca ggcatcgcca ctgccatagc gctggttgag
     3061 taaataagcg acaaacgcca atgctgcagc gagggtacag ccacgagacg gcgtggtaaa
     3121 caaggcttta agatgaggga aaatgaaagc cagtgcgccg aggataaaga acggtaaata
     3181 aaatagcgtt tgcatgacaa taaaattgaa catgccatta ctcagaatgg gtggatacac
     3241 aataaaaatc gttcttctta ttaccgcata gccgatgccg aggcataaaa aaatcaccga
     3301 tagttttacc atcgagaatt ttttattcgt tttatcagaa ttttctaaat tatttctgat
     3361 gcgcttaaat atccatacac acagcgtcgt catgactact aacaccagta aaaaccacag
     3421 gtgtgatatt aattcccagg ccaacgtatt atatttgtca tacaatgata gcccaggcca
     3481 actttccgct ttccctttga catattgcag cataataaat tgcggtaatg tcagtagggg
     3541 gatggctgtt aacatcggga tacctacacg ttcgacacgt actttccacc attttttcaa
     3601 gggatagcgt aaaaaaagca tgtaggaaaa gtatccggat ataacgaaaa atacctgcat
     3661 gcggaacgag tggatgaagt cattaaaaag ggttagccac aatgacggtt cggcgctatt
     3721 cacatgccat gtatggctcg aatagattaa agaaatatga aaagggatcc ctaacaacat
     3781 cagccaggcg cggatggagt cgaggaaata ttcacgttgc gcgggtactg gattcatata
     3841 tggttaacta atctcggatt tttcgtctta tccctgtcgg gttatgcctt taggcttgtt
     3901 gccatagcga caccgacctg accgcgccag gcgcaggctt caaggttttt atgcatagca
     3961 tcatcgctac cactaaccag aatggaagcg tctgtaagac ggttgataaa taaatttgct
     4021 ggcaaaccct acacgaagtc gatgcttctg tctttaggag aagcacggaa agtgaaaacg
     4081 gttgcaatca ggtgcttaat ccatgagcca gcgtgctgaa cgataccggg attcagttgt
     4141 cggaatggct ggttatccat taaaatagat cggatcgata taagcacaca aagggggaag
     4201 tgcttactaa ttatgaaaca taaactacaa atgatgaaaa tgcgttggtt gagtgctgca
     4261 gtaatgttaa ccctgtatac atcttcaagc tgggctttca gtattgatga tgtcgcaaag
     4321 caagctcaat ccttagccgg gaaaggctat gaggcgccta aaagcaactt gccctccgtt
     4381 ttccgcgaca tgaaatacgc ggactatcag cagatccagt ttaatcatga caaagcgtac
     4441 tggaacaatc tgaagacccc attcaaactc gagttctacc atcagggtat gtacttcgat
     4501 accccggtca aaataaatga agtgactgcc accgcagtca aacgaatcaa atacagcccg
     4561 gattatttca ctttcggcga tgttcagcat gacaaagaca cggtaaaaga ccttggtttt
     4621 gccggtttta aagtgcttta cccgatcaac agcaaagata aaaacgatga aatcgtcagc
     4681 atgctcgggg ccagctattt ccgcgtgatt ggtgcaggtc aggtttatgg cctttctgcc
     4741 cgcggcctag caattgatac cgctttgcca tcgggtgaag aatttccacg cttcaaagag
     4801 ttctggatcg agcgtccaaa accgactgat aaacgtttaa ccatttatgc attgcttgac
     4861 tcgccgcgtg cgacaggtgc ttacaaattc gtggttatgc cagggcgtga cacggttgtg
     4921 gatgtgcagt cgaaaatcta tctgcgcgat aaagtcggca aactgggggt tgcaccgtta
     4981 accagtatgt tcctgtttgg gccgaaccaa ccgtcgcctg caaataacta tcgtccggag
     5041 ttgcacgact ctaacggtct gtctatccat gctggtaatg gcgaatggat ctggcgtccg
     5101 ttgaataacc cgaaacattt agcggtcagc agcttctcca tggaaaaccc gcgaggcttt
     5161 ggtctgttgc aacgaggtcg tgatttctcc cgctttgaag atctcgatga tcgttacgat
     5221 ctccgtccaa gcgcatgggt gactccgaaa ggggagtggg gcaaaggcag cgttgagctg
     5281 gtggaaattc caaccaacga tgaaaccaac gataacatcg tcgcttactg gacgccggat
     5341 cagctgccgg agccgggtaa agagatgaac tttaaataca ccatcacctt cagccgtgat
     5401 gaagacaaac tgcatgcgcc agataacgca tgggtgcaac aaacgcgtcg ttcaacgggg
     5461 gatgtgaagc agtcgaacct gattcgccag cctgacggta ctatcgcctt tgtggtcgat
     5521 tttaccggcg cagagatgaa aaaactgcca gaggataccc cggtcacagc gcaaaccagc
     5581 attggtgata atggtgagat agttgaaagc acggtgcgct ataacccggt taccaaaggc
     5641 tggcgtctgg tgatgcgtgt gaaagtgaaa gatgccaaga aaatcactga aatgcgtgct
     5701 gcgctggtga atgccgatca gacgttgagt gaaacctgga gctaccagtt acctgccaat
     5761 gaataagaca actgagtaca ttgacgcaat gcccatcgcc gcaagcgaga aagcagcatt
     5821 gccgaagact gatatccgcg ccgttcatca ggcgctggat gccgaacacc gcacctgggc
     5881 gcgggaggat gattccccgc aaggctcggt aaaggcgcgt ctggaacaag cctggccaga
     5941 ttcacttgct gatggacagt taattaaaga cgacgaaggg cgcgatcagc ttaaggcgat
     6001 gccagaagca aaacgctcct cgatgtttcc cgacccgtgg cgtaccaacc cggtaggccg
     6061 tttctgggat cgcctgcgtg gacgcgatgt cacgccgcgc tatctggctc gtttgaccaa
     6121 agaagagcag gagagcgagc aaaagtggcg taccgttggt accatccgcc gttacattct
     6181 gttgatcctg acgctcgcgc aaactgttgt cgcgacctgg tatatgaaga ccattcttcc
     6241 ttatcagggt tgggcgctga ttaatcctat ggatatggtt ggtcaggatt tgtgggtttc
     6301 ctttatgcag cttctgcctt atatgctgca aaccggtatc ctgatccttt ttgcggtact
     6361 gttctgttgg gtgtccgccg gattctggac ggcgttgatg ggcttcctgc aactgcttat
     6421 tggtcgcgat aaatacagta tatctgcgtc aacagttggc gatgaaccat taaacccgga
     6481 gcatcgcacg gcgttgatca tgcctatctg taacgaagac gtgaaccgtg tttttgctgg
     6541 cttgcgtgca acgtgggaat cagtaaaagc caccgggaat gccaaacact ttgatgtcta
     6601 cattctgagt gacagttata acccggatat ctgcgtcgca gagcaaaaag cctggatgga
     6661 gcttatcgct gaagtcggtg gcgaaggtca gattttctat cgccgccgcc gtcgccgcgt
     6721 gaagcgtaaa agcggtaata tcgatgactt ctgccgtcgc tggggcagcc agtacagcta
     6781 catggtggtg ctggatgctg actcggtaat gaccggtgat tgtttatgcg gcctggtgcg
     6841 cttgatggaa gccaacccga acgccgggat cattcagtcg tcgccgaaag cgtccggtat
     6901 ggatacgctg tatgcgcgct gtcagcagtt cgcgacccgc gtgtatgggc cactgtttac
     6961 agccggtttg cacttctggc aacttggcga gtcgcactac tggggacata acgcgattat
     7021 ccgcgtgaaa ccatttatcg agcactgcgc actggctccg ctgccgggcg aaggttcctt
     7081 tgccggttca atcctgtcac atgacttcgt agaagcggcg ttgatgcgcc gtgcaggttg
     7141 gggggtctgg attgcttacg atctcccggg ttcttatgaa gaattgccgc ctaacttgct
     7201 tgatgagcta aaacgtgacc gccgctggtg ccacggtaac ctgatgaact tccgtctgtt
     7261 cctggttaag ggtatgcacc cggttcaccg tgcggtgttc ctgacgggcg tgatgtctta
     7321 tctctccgct ccgctgtggt ttatgttcct cgcgctctct actgcattgc aggtagtgca
     7381 tgcgttgacc gaaccgcaat acttcctgca accacggcag ttgttcccgg tgtggccgca
     7441 gtggcgtcct gagctggcga ttgcactttt tgcttcgacc atggtgctgt tgttcctgcc
     7501 gaagttattg agcattttgc ttatctggtg caaaggaacg aaagaatacg gcggcttctg
     7561 gcgcgttaca ttatcgttgc tgctggaagt gctgttttcc gtgctgctgg ctccggtacg
     7621 catgctgttc catacggtct tcgttgtcag cgcgttcctt ggctgggaag tggtgtggaa
     7681 ttcaccgcag cgtgatgatg actccacttc atggggcgaa gcgttcaaac gccacggctc
     7741 acagctgctg ttggggttag tgtgggctgt tgggatggcg tggctggatc tgcgtttcct
     7801 gttctggctg gcaccgattg tcttctcgtt gatcctgtca ccgtttgttt cggtgatttc
     7861 cagccgtgcc accgttggtc tgcgcaccaa acgctggaaa ctgttcctga tcccggaaga
     7921 gtattcaccg ccgcaggtgc tggttgatac cgatcggttc cttgagatga atcgccaacg
     7981 ctcccttgac gatggcttta tgcacgcagt gtttaacccg tcatttaacg ctctggcaac
     8041 cgcaatggcg acagcgcgtc accgcgccag taaggtgctg gaaatcgccc gtgaccgcca
     8101 cgttgaacag gcgcttaacg agacgccaga gaagctgaat cgcgatcgtc gcctggtgct
     8161 gctaagcgat ccggtgacga tggcccgtct gcatttccgc gtctggaatt cccctgaaag
     8221 atattcttca tgggtgagtt attacgaagg gataaagctc aatccactgg cattgcgtaa
     8281 accggatgcg gcttcgcaat aaaaacgtag ttgcctgatg cgctacgctt atcaggccta
     8341 catcgttcct gcaatttatt gattttgcaa gattttgtag gtcggataag gcgttcacgc
     8401 cacatccggc aaaaacaacg agccaataaa aataccggcg ttatgccgta atgccagtca
     8461 gttaagcaac tgactggctc tttttcgggg ctgtggggta tttccagggc ctctccttta
     8521 ccactctc
//
LOCUS       DAZPCS010000153         8502 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZPCS010000153 DAZPCS010000000
VERSION     DAZPCS010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8502
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.154"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            45..398
                     /locus_tag="ACHUQ7_003325"
     CDS             45..398
                     /locus_tag="ACHUQ7_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFR1136601.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(435..2513)
                     /gene="flhA"
                     /locus_tag="ACHUQ7_003326"
     CDS             complement(435..2513)
                     /gene="flhA"
                     /locus_tag="ACHUQ7_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HFR1136602.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAGGHIFGGCEFIRYDERPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     YERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGSGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGANAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNSVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(2587..3597)
                     /gene="hypE"
                     /locus_tag="ACHUQ7_003327"
     CDS             complement(2587..3597)
                     /gene="hypE"
                     /locus_tag="ACHUQ7_003327"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFR1136603.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAGSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(3594..4715)
                     /gene="hypD"
                     /locus_tag="ACHUQ7_003328"
     CDS             complement(3594..4715)
                     /gene="hypD"
                     /locus_tag="ACHUQ7_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFR1136604.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            complement(4715..4987)
                     /gene="hypC"
                     /locus_tag="ACHUQ7_003329"
     CDS             complement(4715..4987)
                     /gene="hypC"
                     /locus_tag="ACHUQ7_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFR1136605.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(4978..5850)
                     /gene="hypB"
                     /locus_tag="ACHUQ7_003330"
     CDS             complement(4978..5850)
                     /gene="hypB"
                     /locus_tag="ACHUQ7_003330"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFR1136606.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHTPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(5854..6204)
                     /gene="hypA"
                     /locus_tag="ACHUQ7_003331"
     CDS             complement(5854..6204)
                     /gene="hypA"
                     /locus_tag="ACHUQ7_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFR1136607.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            6416..6877
                     /gene="hycA"
                     /locus_tag="ACHUQ7_003332"
     CDS             6416..6877
                     /gene="hycA"
                     /locus_tag="ACHUQ7_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFR1136608.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            7002..7613
                     /gene="hycB"
                     /locus_tag="ACHUQ7_003333"
     CDS             7002..7613
                     /gene="hycB"
                     /locus_tag="ACHUQ7_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFR1136609.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            7610..8502
                     /locus_tag="ACHUQ7_003334"
                     /pseudo
     CDS             7610..8502
                     /locus_tag="ACHUQ7_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
BASE COUNT         1905 a         2309 c         2288 g         2000 t
ORIGIN      
        1 gattaagatg taatataaat gcatcttaat gaggtggagg cagcatgtcc ggcaagcgta
       61 tctctcgtga aaaactgacg attaaaaaaa tgatcgatct ttatcaagcg aaatgcccgc
      121 aggcgtcagc ggagccggag cattacgagg cgttgtttgt ttacgcgcaa aagcggctgg
      181 ataaatgtgt gttcggcgaa gagaaaccag cctgtaagca gtgtccggtg cactgttacc
      241 agcctgcaaa gcgtgaagag atgaaacaga ttatgcgttg ggccgggccg agaatgctgt
      301 ggcgtcatcc gatcttaacc gtgcgtcatc tcatcgatga caagcgtccg gtgccagaac
      361 tgccggaaaa atatcgtccg aaaaaacctc gtgaatagta tgccggacag gcagatctgt
      421 ccggcaattt gcagttaaat caatgccgat ttatcaattc ccagccgctt catccgtgac
      481 agcagggtcg tgcgtttcag ccccagacgt tgcgcagcgc ctttaggccc ggcaaccacg
      541 ctgttggttt ctttcagcac gcgcacaatc aactgatatt catcttcgcc ctcctgggcg
      601 acaaccgttg cggcaggcgg cgtttcaggc tccggtaaag caatatctgg caatgacagc
      661 tgcagcacgt tgccgcgtgt tagcaatact gcgcgctcaa tgacgttttc cagttcgcgt
      721 acgttacccg gccactccat gttgctcaag gtgcgcagcg tctcggcagg aatgctgtcg
      781 atattgcgcc ccagacgacg ggcaattttg aaggtaaagg ctttcgccag cagcggaata
      841 tcttccggac gctcgcgtag tggcggcagg tgaatcggga atacgttcag gcggtaatag
      901 agatcgctac ggaactctcg gtcggcgacc atttttttca gatcgcggtt agtcgcggca
      961 attaaacgta cgtccgtctg aatgattttg ttgctgccga gacgttcaaa ctcctgttcc
     1021 tgcaatacac gcagcaactt cggctgtaac tccagtggca tatcgcccac ttcgtcgagg
     1081 aacagagagc ttttatccgc cagttcaaaa cgaccaatgc gctgggcgtt ggcaccggta
     1141 aaagccccac gctcatgacc aaacagatcg ctttccagca atccggcagg catcgccgcg
     1201 cagttcattt tgaccatgcg gcgattatta cgcccactga gattatggat cgcacgggca
     1261 atcagctctt tacccgagcc agtttcaccg aggatcagca cggtactgtc actttgcgcc
     1321 accatttcaa cttgtttaag cacgctgtac atggcttcgc tgcggccaat aatctcgcca
     1381 aattcactat caacattgtt gagctgctcg gtcagggcga ggttttcatc aaccagccgt
     1441 tctttcagac gatggatttc ctgataggcg agggcgttat cgacagcgat tgccacacgt
     1501 tcggcaatct ggcgcagcaa attcagattg gtagtggtaa acactttctc ttcgcattgc
     1561 gccagtttca gcacgcccag catggtgtcg ccagacatca gcggtaacag gcacaaggtt
     1621 tgaatctggt tgccccaggt gtcgaacaac atgcgttcat agggggctaa atcgtcccgc
     1681 tcgtagagat tgatcagcag catctcttta cttttgaaca cgcgttcggt gagggttccg
     1741 gcttcatcga cttcgctctg ttcgtgggcg ggatgttgtt tatcaagata gtgagtggag
     1801 tagatgttga gtttgttttt acggtggctg cgtaagacaa tgctgatatc gtcaatgtcg
     1861 aaatagtaat ggatttcttt ggcgacttcg ctgaccagtt cgtccatatc caggcgggaa
     1921 agcaccgcgt tggtaatggc aaccaggatg cggaagttat cgcgctcccg gcataacaac
     1981 tcatagtcga cattgttgac gacgcggctc tggatttgtt cggtgacgac agaaacgatc
     2041 tgcgtaaatg tttgcagacg attgaactct ttttcgctcc agggacgctc gtcataacga
     2101 ataaattcac agccaccaaa aatatgccct cccgccgcca gtggcatcag gcaatagtga
     2161 ccaaatttgg ggtatagccc acctgcggcc agctgcggcc aggtttcaca aaattcttcg
     2221 taactgcaat gcagcgtatc agggcgcgac aaaatgctgc gtaccggacc gtgtgccaga
     2281 acagtttcgt cttcatattt aatgggggtg tctttttcac gcgacgcgta ataagacgca
     2341 cgttgagtct gcgcttgcca caacacaata gccgcgttgt cggcgagcgc agaacgcttt
     2401 accagttgcg aaagagcctc acacagcgag gccagatcgg gctgctgcaa tagtgtccga
     2461 gtgatgtcga acaacccttg ctgtccgaga tcactcatcg gtgtatatga cattttgttc
     2521 ttccaggaaa gcaccgcaaa gcggtgcaaa aggtcattga ctaactatag gagatttaga
     2581 attttattag catatacgcg gaagcggttc ggcgtgtggt aaatcgaggg ttcgtttcac
     2641 gccatacaga ccggcaagac gaacaccttt acgttccacc acttcaccaa tcagcgccgc
     2701 gtctttcccc agtggatggg aatgtaacgc tgccagcact tgctctgccg cgttgcgttc
     2761 aacggcgatc accagtttgc cttcgttggc aaagttcagg gcgtccagtc ccagcaattc
     2821 gcaaacgccg cgcacggcag gtttaaccgg cagcgctgat tcagaaattt caataccaca
     2881 accgcaggct gccgcgaact catgaaccac cgcgtttaca ccaccacggg tggcatcacg
     2941 cagcgctttc acgccgggaa tgtcacgcag cgtctgaata agcggcgtca gcaccgcgca
     3001 gtcgctgacc agttcgccat ccagccccag ctgctcacgc aggttaagga tagtcgcccc
     3061 gtggtcgccg agtgtaccgc taaccagcaa tacatcgcct gcggttagcg tctgtgcgcc
     3121 ccagtgaata ttcgccggaa ttgcgcccat gccagcggtg ttgataaaca gtttatctgc
     3181 cgcgccgcgc tgcaccactt tagtatcgcc agtaacgatg gcaatgcctg ccgtgcgggc
     3241 ggtttctgcc atgctggtca ctacggcttt cagtgtctcc atcggcaatc cttcttcgag
     3301 gataaagcca caggagagat agcgcggaat agcgccactg cccgcaacgt cattcgcggt
     3361 gccgcaaatc gccagcttgc cgatattacc gccaggaaag aacagcgggt caatgacgta
     3421 actgtcggtg gagaacgcca gacggtcgcc ttccgctacc agctgcgcca gatcaagacg
     3481 tgcctgatct tcctgctctg ccagccacgg gttggcaaag gcttccataa acaggctgtt
     3541 gattaattgc tgcatcgcct ggccgccgct gccgtgggcg agttggatat tattcacgct
     3601 tcactctcct gctgacgata ctgataccac gcggcgcacg ctccttcgga ggagaccatc
     3661 agtgcaccaa atgcggtttg aggattacag gtgttaccaa acagcgggca ttgatgcggc
     3721 ttacatttgc ccgttaatac ctcaccacaa cgcgcgcgcg ggtcatcgca gacctgctgc
     3781 ggtgccgggc ggaaatgtgc ttcggcatcg aatcgttgat aatccggcgt caggtgcacg
     3841 ccagaagatt caatcacgcc taagccgcgc cattcgctgt cgccgttgac acagaacaca
     3901 tcggcaatcg cctgttgcgc cagtaggtta ccggcatccg gtaccacccg acgatactga
     3961 ttctctacct tgctgtgggc cgctattttc tgctccacca gcatgaccac gccttgcagt
     4021 agatcaaggg gttcgaaacc agcgaccacc agcggacgat gaaaatcgct ggcgataaaa
     4081 ttataggcat cagtgccgat aaccatacta acgtggcccg gcgcgaggaa cgcgtcgata
     4141 ccgttatccg gctgttccag caaactgcgc agcgtcggga taagcgtaat gtgctggcag
     4201 aagaagtaaa aattctgcac atcacgcgct ttcgcctgtt gcagagtgat ggcggtggtc
     4261 ggcatggtgg tttcaaaacc taagccgaag aacaccactt tgcgggttgg attctcctgc
     4321 gccagtttca acgcatccat cggcgagtaa acgatgcgca catcggcacc gcgtgctttt
     4381 gcctgcaaca gcgatccctg tttccctggt acgcgcatgg cgtcgccaaa ggtacagaag
     4441 atgacttccg gatggctggc aatctccacg caggtgtcga ttctacccat cggcagtacg
     4501 cacaccgggc aacccggacc gtggataaac tcaacgtttt caggcagtaa ctggtcgagg
     4561 ccgaatttaa aaatggcgtg ggtatgaccg ccacacactt ccataatccg cagagggcgt
     4621 tcggcggtgt aagagagatg tgaagcacgt tcgcgcagat gctcaattaa ctgcatcacc
     4681 tgttccggcg cgcgatattc atcaacaaaa cgcattattt ttcctcgcca tacaacagcg
     4741 cgccgacatc cggctcaacg tcaaacatgt tttgtaaggc gtcgagagtg tcgcgtgctt
     4801 cggcttcatt aattacgctc atggcaaagc caacgtgaac cagtacccac tggcccacgc
     4861 gcggctgacc gttttcatcg cagctgccga ctaacgttaa atcgacatcg cgctgaatgc
     4921 cgcagacgtc gactttcgcc tggttgccgt caatggtgcg gatctggccg ggaacgccta
     4981 tgcacatcgc tgtgtctcca gccagttcag ccactggtcc atcccttcgc cgctggtggc
     5041 ggaaataagg atgatttcaa tttctggatt gacttcgcgg gcgcaggcga tgcacttctc
     5101 aacgtcaaag ttgagatacg gcaacaggtc aactttgttg agcagcatca gcgaggcggc
     5161 ggcaaacata tgcggatatt tcagcggttt gtcttcacct tcggtaacgg aaagcaccgc
     5221 tactttgtgt ttttcaccga gatcgaagct ggccgggcat acgaggttgc caacgttttc
     5281 gataaacaga ataccgttat cgtccagtgg cagacgcggt gcggcgtcgg caatcatctg
     5341 tgcgtcaaga tggcagcctt taccggtgtt cacctgaatc gctggtgtac cggtagcgcg
     5401 aatgcgtgcg gcatcgttca cggtttgctg gtcgccttca ataaccgcgc acggaacgct
     5461 gtctttcagg cgcattaagg tttccgtcag cagggtggtt ttaccggaac cagggctgga
     5521 aaccaggttg agcaccagtt gcttgcgggc agcaaagcgc gcgcggttgc gttcagccag
     5581 acggttgttt ttgtccagca cgtcaatttc gacttccagc atccgacgct ggctcatgcc
     5641 cggtgtgtga gtgcccgctt cgccatgacc gtaatgcagg tcgccttctt cagtctggct
     5701 gggggtaaat tcaggcgctt taatgccggt gattttcatc tgcgggcgtg ccgccggggc
     5761 aaatggcgcg ctacgaaacg cggaatgagg gttatgttca tcaccctcga tatacaggtt
     5821 gccttcaccg caaccgcatg ttgtacacat cgctcactcc tggtctattt ctatccgccg
     5881 aatctgtaaa ccgtcgtctg ccacaatctg tagcatgtca ccatgacact gtggacagcg
     5941 gcggacgcgc tgggtcagta gcgtcacata ctgttggcat gtttcacacc agcattcggc
     6001 ctcttgttct tcgaggtgca gtttacaacc ttccgccacg ctgccgcggc aaaccagatc
     6061 aaaacaaaag gcaagagagc tggtttcgac acaagaaaat gcgccaattt tgagccagac
     6121 cccagttacg cgttttgcgc cgtgttttgc ggcctgctgt tcgatcaatt ccagtgcccg
     6181 ttggcagagg gttatttcgt gcatatcgcc tcccattaac tattgccagc tacaagcaat
     6241 aattgcgcca gtgttgatta tccctgcggt gaataatgtc gatgatgtcg aaatgacacg
     6301 tcgacacggc gacgaaattc atctttagct taaaaatctc tttaataaca ataaattaaa
     6361 agttggcaca aaaaatgctt aaagctggca tctctgttaa acgagtaacc tgacaatgac
     6421 tatttgggaa ataagcgaga aagccgatta catcgcacag cggcatcgtc gcctacagga
     6481 ccagtggcac atctactgca attcgctggt tcaggggatc acgttatcga aagcgcgcct
     6541 gcatcacgcc atgagctgcg cgccggacaa agaactctgt ttcgtccttt ttgaacattt
     6601 tcgtatttac gtcaccctgg cggatggctt taacagccac accatcgagt attacgtcga
     6661 aacaaaagat ggcgaagaca aacagcggat tgcgcaggcg caactgagca ttgacggcat
     6721 gattgatggc aaggtcaaca tccgcgatcg cgaacaggtt ctggaacact atctcgaaaa
     6781 aatcgctggc gtttacgaca gcttatacac cgccattgaa aacaatgtgc cggtgaattt
     6841 aagccaactg gtaaagggac aaagcccggc agcatgagct gaggctttgc ccgttttgca
     6901 ggcgttacgc ctgtttgggg atgggcgtgt cgatgagtgt cgaaaatgac atttcatcgg
     6961 catgttttcg tcaaaaatga caatcacctg aggaatgcct ggtgaatcgt tttgtaattg
     7021 ccgactccac gctctgtatc ggctgccaca cctgtgaggc cgcctgttca gagacgcatc
     7081 gccagcacgg cctgcaatca atgccgcgcc tgagagtgat gctcaatgaa aaagaatctg
     7141 cgccgcagct ctgtcaccac tgtgaagatg caccctgcgc ggtggtctgc ccggttaacg
     7201 ccatcacccg cgttgatggg gccgtgcagt tgaatgaaag tctgtgcgta agctgcaaac
     7261 tgtgcggcat cgcctgcccg tttggcgcaa ttgaattttc cggcagccgt ccgctggata
     7321 ttccggcaaa cgccaatacc ccgaaagcgc caccggcacc gcctgctccg gcgcgtgtca
     7381 gcacattgct tgactgggtg ccaggtattc gcgcgatcgc cgtcaaatgt gacctttgta
     7441 gctttgatga acaaggtccg gcctgtgtgc ggatgtgccc gactaaagcc ctgcatctgg
     7501 tggataacac cgatatcgcc cgcgtcagca aacgtaagcg tgagctgacc tttaacacgg
     7561 actttggcga tctcaccttg tttcagcagg ctcaaagtgg agaggctaaa tgagcgcaat
     7621 ttccctgatc aatagcggcg tggcatggtt tgtcgccgcc gctgttctgg catttctctt
     7681 ttcaaaaagc gctaagtggc tggatagccg gaattggcgg cgcggttggt agtctgtata
     7741 cggcagccgc gggcttcact gtactgactg gcgcggttgg cgtgagcggt gcgctgtcgc
     7801 tggtaagcta cgatgtgcaa atctccccgc ttaacgcgat ttggctgatt accctcggcc
     7861 tttgcggtct gtttgtcagc ctctataaca ttgactggca tcgccacgcg caggtgaagt
     7921 gcaacggctt gcagatcaat atgttgatgg ctgccgccgt ctgcgccgtc atcgccagca
     7981 acctcggcat gttcgtggta atggccgaag tcatggcctt gtgtgcggtg ttcctcacca
     8041 gcaacagcaa agagggcaaa ctgtggtttg cgctgaggcg tcttggcact ctgctgctgg
     8101 tgattgcttg ctggctgctg tggcagcgtt atggcacgct ggatctgcgc ctgctggata
     8161 tgcgtatgca acagctgccg ctcggttccg atatctggct gctcggtgtg attggctttg
     8221 gcctgctggc tgggattatt ccgctgcacg gctgggtgcc gcaggcacat gctaacgcct
     8281 ctgcgccagc tgccgcgttg ttttctacgg tagtcatgaa aattggcctg ctgggcatgt
     8341 taaccctgtc actgctgggc ggtaatgcac cgctgtggtg ggggatcgcg ctgctggtgc
     8401 tcggcatgat caccgcgttc gtcggtggtc tgtatgcgtt gatggaacac aacatccagc
     8461 gcctgctggc ttaccacacc ctggaaaata tcggcattat cc
//
LOCUS       DAZPCS010000154         8392 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZPCS010000154 DAZPCS010000000
VERSION     DAZPCS010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8392
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.155"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            310..1242
                     /gene="glaH"
                     /locus_tag="ACHUQ7_003335"
     CDS             310..1242
                     /gene="glaH"
                     /locus_tag="ACHUQ7_003335"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFR1136610.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDDWEHLDHYFRHPLARRPMRFAAPPSKNVSKDVFHPVFDV
                     DQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVPVPVGKFLLINNLFWL
                     HGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            1262..2530
                     /gene="lhgO"
                     /locus_tag="ACHUQ7_003336"
     CDS             1262..2530
                     /gene="lhgO"
                     /locus_tag="ACHUQ7_003336"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFR1136611.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            2553..4001
                     /gene="gabD"
                     /locus_tag="ACHUQ7_003337"
                     /pseudo
     CDS             2553..4001
                     /gene="gabD"
                     /locus_tag="ACHUQ7_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
     gene            4009..5295
                     /gene="gabT"
                     /locus_tag="ACHUQ7_003338"
                     /pseudo
     CDS             4009..5295
                     /gene="gabT"
                     /locus_tag="ACHUQ7_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307963.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
     gene            5533..6933
                     /gene="gabP"
                     /locus_tag="ACHUQ7_003339"
     CDS             5533..6933
                     /gene="gabP"
                     /locus_tag="ACHUQ7_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFR1136612.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAD
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDMPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLDPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            6954..7616
                     /gene="csiR"
                     /locus_tag="ACHUQ7_003340"
     CDS             6954..7616
                     /gene="csiR"
                     /locus_tag="ACHUQ7_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFR1136613.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLQ
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLPRDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7617..8066)
                     /gene="kbp"
                     /locus_tag="ACHUQ7_003341"
     CDS             complement(7617..8066)
                     /gene="kbp"
                     /locus_tag="ACHUQ7_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFR1136614.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(8150..8308)
                     /locus_tag="ACHUQ7_003342"
     CDS             complement(8150..8308)
                     /locus_tag="ACHUQ7_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFR1136615.1"
                     /translation="MGFWRIVITIILPPIGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
BASE COUNT         1899 a         2200 c         2331 g         1962 t
ORIGIN      
        1 acggggcaga taatatgttt agtttactaa cggtcatttt gtagtgaagc catttactgt
       61 tttttatcga ccagataatc tgttctctaa tgttaactcc ccctaacctg ttgctttagt
      121 tattcatttc ctgtctcact ttgccttaat accctacgtt aaatgttact aatttgttgc
      181 ttttgatcac aataagaaag caatatgtcg cttttgtgcg catttttcag aaatgtagat
      241 atttttagat tatggctacg aaatgagcat cgccatgtca ccctacatct cataagagga
      301 tcgcttctga tgaatgcact gaccgccgta caaaataacg ctgtcgattc aggccaggac
      361 tatagcggat tcactctcat cccgtcggcg caatccccgc gtctgctgga actcaccttc
      421 accgaacaga cgaccaaaca gtttctcgaa caggttgccg aatggcccgt gcaggcgctg
      481 gagtacaaat cgtttctgcg ttttcgggta ggcaaaattc ttgacgatct gtgtgcgaat
      541 cagctgcaac cattgctgtt gaagaccctg ctaaaccgtg ctgaaggtgc gctgttgatc
      601 aatgcggtag gtatcgatga tgtggcgcag gcagatgaga tggtgaagct ggcaacggcg
      661 gtagcgcatc tgattggtcg ctcaaatttc gatgcgatga gcggtcagta ttacgcgcgt
      721 ttcgtggtga aaaatgtcga taactcagac agctatctgc gtcagccgca ccgcgtgatg
      781 gaactgcaca acgacggcac gtatgtcgaa gagatcaccg attacgtgct gatgatgaaa
      841 atcgatgact gggaacatct ggaccactat ttccgccatc cactggcgcg tcgcccgatg
      901 cgctttgccg cgccgccgag caaaaacgtc agcaaagatg ttttccatcc agtgttcgac
      961 gttgatcaac agggtcgccc ggtgatgcgc tatatcgacc agttcgtcca gccaaaagat
     1021 tttgaagaag gcgtgtggtt gagcgagctt tccgatgcca ttgaaaccag caaaggcatt
     1081 ctttctgtac ccgttcccgt tggcaaattc ctgttgataa acaacctgtt ctggctgcac
     1141 ggtcgcgacc gctttactcc gcacccggat ctgcgccgtg aactgatgcg tcagcgtggc
     1201 tatttcgctt acgccactca ccactaccag acgcatcagt aagcgcaaag gaattgagcg
     1261 gatgtatgat tttgtgatta ttggcggcgg catcatcggc atgtcgaccg ccatgcaact
     1321 gattgatgtt tatccggatg cccgcattgc gttgctggaa aaagagtccg gcccggcctg
     1381 tcaccagacg ggccacaaca gcggcgtgat ccatgccggg gtctattaca cgcccggcag
     1441 cctgaaggcg cagttttgcc tggcggggaa ccgtgccacc aaagcctttt gcgatcaaaa
     1501 cggcattcgc tatgacaact gcggcaagat gctggtcgcc acgtctcagc tcgaaatgga
     1561 acggatgcgc gccttatggg aacgcacagc ggcgaacggt atcgagcgcg agtggataaa
     1621 cgccgtggaa ctgcgcgagc gcgaaccgaa tatcaccggg cttggtggca tttttgtgcc
     1681 gtccagcggc attgtcagct accgcgaagt gacggcggca atggcaaaaa tcttccaggc
     1741 cagaggcggc gagattattt ataacgccga agtcagcgcc ctcagtgagc ataaaaacgg
     1801 cgtggtgata cgcacccgtc agggcggtga atatgaagca tcgacgctga ttagctgttc
     1861 cgggctgatg gctgaccggc tggtgaaaat gctcggcctc gaaccgggct ttattatctg
     1921 cccgttccgt ggcgagtatt tccgccttgc gccggagcat aaccagattg ttaaccacct
     1981 gatttacccc attcccgacc ccgcgatgcc atttttgggc gtgcatctca cccgcatgat
     2041 cgacggcagc gtgaccgtcg ggccaaacgc ggtgctggct ttcaaacgcg aaggctatcg
     2101 caagcgcgac ttctcattta gtgacacgct ggagattttg agctcgtcgg ggattcgccg
     2161 ggtgctgcaa aaccatctac gctcaggact gggcgagatg aaaaactcgc tgtgcaaaag
     2221 cggctatctg cggctggtgc aaaagtattg tccccggctt tcgttaagcg atctccagcc
     2281 ctggcccgcc ggtgtgcggg cgcaggcagt atcgccggac ggcaagctga ttgacgattt
     2341 tctgtttgtc accaccccac gcacgatcca cacctgcaat gcgccctccc cggcagcgac
     2401 atcagcaatt cctattggtg cgcatattgt cagcaaggta caaacgctgt tggcaagcca
     2461 gagtaacccc ggacgcacgc tgcgagcggc acgtagtgtg gatgccttac acgccgcatt
     2521 taatcaataa cctttgaaaa caggacgtaa caatgaaact taacgacagt aacttattcc
     2581 gccagcaggc gttgattaac ggggaatggc tggacgccaa caatggtgaa gtcatcgacg
     2641 tcaccaatcc ggcgaacggc gacaagctgg gtagcgtgcc gaaaatgggc gcagatgaaa
     2701 cccgcgccgc tatcgacgcc gccaaccgcg ccctgcccgc ctggcgtgcg ctcaccgcca
     2761 aagaacgcgc caccattctg cgcaactggt tcaatttgat gatgcagcat caggacgatt
     2821 tagcgcgcct gatgaccctc gaacagggta aaccgctggc tgaagcgaaa ggcgaaatca
     2881 gctacgccgc ctccttcatt gagtggtttg ctgaagaagg caaacgtatt tatggcgaca
     2941 ccattcctgg tcatcaggcc gataagcgcc tgattgttat caagcagccg attggcgtca
     3001 ccgccgccat cacgccgtgg aactttccgg cggcgatgat tacccgtaaa gccggtccgg
     3061 cgctggcggc aggctgcacc atggtgctga agcccgccag tcagacgccg ttctctgcgc
     3121 tggcgctggc ggaactggcg attcgcgcgg gcattccggc tggcgtgttt aacgtggtca
     3181 ccggttcggc aggcgtggtc ggtaatgaac tgaccagcaa cccgctggta cgcaaactgt
     3241 cgtttaccgg ttagaccgag attggccgcc agttaatgga acagtgcgcg aaagacatca
     3301 aaaaagtgtc gctggagctg ggcggcaacg cgccgtttat cgtctttgac gatgccgatc
     3361 tcgataaagc cgtggaaggc gcgctggcct cgaaattccg caacgccggg caaacctgcg
     3421 tctgcgccaa ccgtttatac gtgcaggacg gcgtgtatga ccgctttgcc gaaaaattac
     3481 agcaggcggg gagcaaactg cacatcggcg acgggctgga gaaaggcgtc accatcgggc
     3541 cgctgatcga tgaaaaagcg gtagcaaaag tggaagagca tattgccgat gcgctggaga
     3601 aaggtgcgcg tgtggtttgc ggcggtaaag cgcacgaacg cggcggcaac ttcttccagc
     3661 caaccattct ggtggacgtt ccggccaacg ccaaagtgtc gaaagaagag acgttcggcc
     3721 ccctcgcccc actgttccgt tttaaagatg aagccgatgt gattgcacaa gccaatgaca
     3781 ccgagtttgg tcttgccgcc tatttctacg cccgtgattt aagccgcgtc ttccgcgtcg
     3841 gcgaggcgct ggagtacggc atcgtcggca tcaataccgg cattatttcc aatgaagtgg
     3901 ccccattcgg cggcatcaaa gcctcgggtc tgggtcgtga aggttcgaag tatggcatcg
     3961 aagattactt agaaatcaaa tatatgtgca tcggtcttta actggagaat gcgaaggaac
     4021 agcaataaag agttaatgca gcgccgcagt caggcgattc cccgtggcgt tgggcaaatt
     4081 cacccgattt tcgctgaccg cgcggaaaac tgccgggtgt gggacgttta aggccgtgag
     4141 tatcttgatt tcgcgggcgg gattgcggtg ctcaataccg ggcacctgca tccgaaggtg
     4201 gtggccgcgg tggaagcgca gttgaaaaaa ctgtcgcaca cctgcttcca ggtgctggct
     4261 tacgagccgt atctggagct gtgcgagatt atgaatcaga aggtgccggg cgatttcgcc
     4321 aagaaaacgc tgctggttac gaccggttcc gaagcggtgg aaaacgcggt aaaaatcgcc
     4381 cgcgccgcca ccaaacgtag cggcaccatc gcttttagcg gcgagtatca cgggcgcacg
     4441 cattacacgc tggcgctgac cggcaaggtg aatccgtact ctgcgggcat ggggctgatg
     4501 ccgggtcatg tttatcgcgc gctttatcct tgcccgctgc acggcataag cgaggatgac
     4561 gctatcgcca gcatccaccg gatcttcaaa aatgatgccg cgccggaaga tatcgccgcc
     4621 atcatgattg agccggttca gggcgaaggc ggtttctacg ccgcgtcgcc agcctttatg
     4681 cagcgtttac gcgctctgtg tgacgagcac gggatcatgc tgattgccga tgaagtgcag
     4741 agcggcgcgg ggcgtaccgg cacgctgttt gcgatggagc agatgggcgt ggcgccggat
     4801 cttaccacct ttgcgaaatc gatcgcaggc ggtttcccgc tggcgggcgt caccgggcgc
     4861 gcggaagtga tggacgctgt tgcaccgggc ggtctgggcg gcacctatgc cggtaacccg
     4921 attgcctgcg tggctgcgct ggaagtgttg aaggtgttcg agcaggaaaa tctgctgcaa
     4981 aaagccaacg atctggggca gaagctaaaa gacggattgt tggcgatagc cgaaaaacac
     5041 ccggagatcg gcgacgtacg cgggctggga tcgatgattg ccatcgagct gtttgaagat
     5101 ggcgatcaca gcaaaccgga cgcaaaactc accgccgaga tcgtggcacg cgcccgcgat
     5161 aagggcctga ttcttctctc ctgcggcccg tattacaacg tgctgcgcat ccttgtacca
     5221 ctcaccattg aagacgctca gatccgtcag ggtctggaga ttatcggtca gtgttttgat
     5281 gaggcgaagc agtagcaccg tccccatgca ggaggcgacg ctgcgcgtct tgtccggcct
     5341 acgagaatcc aagtcggata aggcgtttac gccgcatccg acaatctgta cgtgaacagg
     5401 aataaatcta tgtcggccgg ataaggcgga gccgctctcc ggcaaaaaga atcaataaca
     5461 attaaacgcg tgacccgacg cgggaaatgt cggggcgctc tcccaagtga cacactttcg
     5521 agaggattca ggatggggca atcatcgcaa ccacacgagt taggcggcgg gctgaaatca
     5581 cgccacgtca ccatgttgtc tattgccggt gttatcggcg caagtctgtt tgtcggttcc
     5641 agcgtcgcca tcgccgaagc ggacccggcg gtattactgg cctatctgtt cgccggatta
     5701 ctggtggtta tgattatgcg gatgctggcg gaaatggcag ttgccacgcc cgataccggt
     5761 tcgttttcca cctatgccga taaagccatt ggccgctggg cgggctatac catcggctgg
     5821 ttgtactggt ggttttgggt actggttatc ccactggaag ccaacatcgc cgccatgatc
     5881 ctgcactcgt gggttccagg cattcccatc tggttatttt ccctcgtcat taccctcgcc
     5941 ttaactggca gtaatttatt aagcgttaaa aactacggcg aatttgagtt ctggctggcg
     6001 ctgtgcaaag tcatcgctat cctggccttt attttccttg gtgcagtcgc aattagcggt
     6061 ttttaccctt atgccgaagt gagcgggatc tcaagattgt gggatagcgg cggctttatg
     6121 cccaacggtt tcggtgcggt attaagcgcg atgttgatca ccatgttctc gtttatgggc
     6181 gcagaaattg tcaccattgc cgccgcggaa tccgacatgc cggaaaaaca tattgtccgc
     6241 gccaccaact cggttatctg gcgtatttct atcttctatt tgtgctctat ttttgtcgtc
     6301 gtggcattaa taccgtggaa tatgccgggg ctgaaagccg ttggatctta tcgctcggtt
     6361 ctggaattgc tcaatattcc ccatgcgaaa ttaatcatgg actgtgtgat attactttcc
     6421 gtaaccagtt gcctgaactc ggccctgtat accgcctcaa ggatgctcta ctccttaagc
     6481 cgtcgcggtg atgctcccgc ggtaatgggt aaaatcaacc gcagtaaaac cccgtacgtg
     6541 gcggtgttac tctccaccgg cgcagcattc ttaacggtgg tggtgaacta ttacgcgcct
     6601 gcgaaggtat ttaaatttct gatcgacagc tccggcgcta tcgccctgct ggtttattta
     6661 gtcatcgccg tttcacagtt gcggatgcgc aaaattctgc gagcagaagg aagcgaaatt
     6721 cgcttgcgaa tgtggcttga tccctggctc acctggctgg tcatcggctt tattaccttt
     6781 gtgttggtag tgatgctatt ccgtccggcg caacagttag aagtgatctc caccggctta
     6841 ttagcgatag ggattatctg taccgtgcca attatggctc gctggaaaaa gctggtattg
     6901 tggcaaaaaa cacccgttca taatacgcgc tgataatgat caggagtcac accatgacca
     6961 ttacgtctct ggatggctat cgctggctga agaacgatat tattcgcggt aattttcaac
     7021 cggatgaaaa attacggatg agtttactga catcgcgtta tgcacttggc gttgggccgt
     7081 tgcaggaagc actttctcaa ctggtggcgg aacggctggt cacggtggtg aatcaaaaag
     7141 ggtatcgggt ggcgtctatg tcagagcagg agctgctcga tattttcgac gcccgcgcca
     7201 atatggaagc gatgctggtc agtctggcga ttgcccgcgg tggcgatgaa tgggaggcag
     7261 acgttctcgc aaaagcgcat ctgctcagta agctggaagc ctgtgacgcc agcgagaaaa
     7321 tgcttgatga gtgggatctg cgtcatcagg cgtttcatac agcaattgtg gcgggctgtg
     7381 gttctcacta tttgctgcaa atgcgtgaac ggttgtttga tctggcggcg cgttatcgat
     7441 ttatctggct gcggcgaacg gtgctttcgg tggaaatgct ggaggataaa cacgatcagc
     7501 accagaccct gactgcggcg gtactgccgc gagatacagc gcgcgccagt gagttaatgc
     7561 gccagcattt actgacgcca attcccatta tccagcaggc gatggctggc aattaactac
     7621 tcttccggaa tacgcaacac ttgccccgga taaattttat ccgggctttt tagcatcggt
     7681 ttattcgctt cgaagatttt attgtacaga ttagcgttac cgtagacctg tttggaaatg
     7741 gcactcagag tgtcgccaga cttaacggta taaaactggc tggcagtggc tggtgtcgcc
     7801 gttttcacct gatcatcaac actggcaata ccggaaatat tccccaccgc aacaaggatt
     7861 ttctccttcg cctcctgact caggccgtca ccagtgaccg tcgctttgcc gtcggcaatt
     7921 tgaatgttca ctttatcggc atcaggtata ccggttttgc tcagatgctc ctgcaccttc
     7981 ttcgcctgat cgtctttatc gtgctgacct gtaaccgcat cccagagttt ttctccggca
     8041 tctttaacaa aattgaacag acccatagct acctcattcg ttaacggaaa cactcagaaa
     8101 gtgtagcaga ggaacgcggc gcgttaacgc cgcgggggtg agcacaggtt taatcgcggg
     8161 tttgcaccca gaacgcgtga atcaaaccgg ggatataccc aagcagtgtc agcagaatat
     8221 taataatgaa cgcccaaccg aaccctttac cgagcagcac gccgatcggc ggcagaatga
     8281 tggtgatgac gattctccag aaacccatat gtactcccta taagaaaatt actcattgtt
     8341 taaaaagaga ttttatctct taagcgtagt aattttagcg gagcctgcca gt
//
LOCUS       DAZPCS010000155         8286 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZPCS010000155 DAZPCS010000000
VERSION     DAZPCS010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8286
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.156"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(414..1610)
                     /gene="hemY"
                     /locus_tag="ACHUQ7_003343"
     CDS             complement(414..1610)
                     /gene="hemY"
                     /locus_tag="ACHUQ7_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFR1136616.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1613..2824)
                     /gene="hemX"
                     /locus_tag="ACHUQ7_003344"
     CDS             complement(1613..2824)
                     /gene="hemX"
                     /locus_tag="ACHUQ7_003344"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFR1136617.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPQ
                     GDTPAAAPQGE"
     gene            complement(2846..3586)
                     /gene="hemD"
                     /locus_tag="ACHUQ7_003345"
     CDS             complement(2846..3586)
                     /gene="hemD"
                     /locus_tag="ACHUQ7_003345"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFR1136618.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3583..4524)
                     /gene="hemC"
                     /locus_tag="ACHUQ7_003346"
     CDS             complement(3583..4524)
                     /gene="hemC"
                     /locus_tag="ACHUQ7_003346"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFR1136619.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLCALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            4911..7457
                     /gene="cyaA"
                     /locus_tag="ACHUQ7_003347"
     CDS             4911..7457
                     /gene="cyaA"
                     /locus_tag="ACHUQ7_003347"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFR1136620.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPRMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(7497..7817)
                     /gene="cyaY"
                     /locus_tag="ACHUQ7_003348"
     CDS             complement(7497..7817)
                     /gene="cyaY"
                     /locus_tag="ACHUQ7_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFR1136621.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7865..>8286)
                     /locus_tag="ACHUQ7_003349"
     CDS             complement(7865..>8286)
                     /locus_tag="ACHUQ7_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFR1136622.1"
                     /translation="FHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTPDLVIDRSF
                     NSGKQHAYFIRQLRSKSLQIIPVNGSLTKNDRFAYDDRNVYTWTDTEVRITPSPCPAK
                     TRVREGNVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
BASE COUNT         1853 a         2180 c         2144 g         2109 t
ORIGIN      
        1 aatgaatttt atcattcata ataagtatgt gtaggatcaa gctcaggtta aatattcact
       61 caggaagtta ttactcagga agcaaagagg attacagaat tatctcataa caagtgttaa
      121 gggatgttat ttcccgattc tctgtggcat aataaacgag tagatgctca ttccatctct
      181 tatgttcgcc ttagtgcctc ataaactccg gaatgacgca gagccgttta cggtgcttat
      241 cgtccactga cagatgtcgc ttatgcctca tcagacacca tggacacaac gttgagtgaa
      301 gcacccactt gttgtcatac agacctgttt taacgcctgc tccgtaataa gagcaggcgt
      361 ttttttatgc atcagaaagg tcccggaggt gctttcctcc gggtgagaag gaactactgt
      421 ggcgggttat tctgcaacgt taacatcaaa ccgtcgcgac gcatcgctgc agcttcttcc
      481 ggcttgtgca gtctgtccag cgcgtcggca agccatgcgt aatcgtaggc gtccggacgt
      541 tgtttcagcg ccgcgcggaa ggcgagcgat gcttcctgcc attcaccgtg cttcatcagt
      601 gactgaccca atgtgctcca caacagcggg cgatcaccga cgtttttgat ttgctggcgt
      661 agcacttttt ccagctgttc cgggttattg gttttcagac gcgggatcgg cagcagtagg
      721 cgatcgtcat actggcgttt caggccgtcg atgataattt gctgggcagt atcatgatcg
      781 tcacattcaa taagatgttc cgccattgcc acctgcaacg ctacctgatg acgcgttttc
      841 cggctttggt ttttccacca gttacgcaaa ccttcactac cgttatcggc acgcgcctga
      901 tccatcaggc caatccatgc ctgttgttcc agcattgcac gatgttcttc atcaccaaca
      961 tgggctttcg ccattgatgg gatgatatcc agcagagaac tccatgcccc tgtgcggata
     1021 tacgcctgtt ccgccagacg taatacttca ggatggcgtg gcgtaacttc cagcagctta
     1081 tccacgccgt ggcgtgcagc atggttttca ttacgggcca gttgcagacg tacacgggtg
     1141 atttctaccg gaatggtgtc attgccggcc agctccgctg cgcgttccag atgttggttg
     1201 gcgcgtgctt catcaccacg ttgttgcgcg gcttcggcag ccagtagata gttcaccacc
     1261 ggttgttcag cgtgatcggc atttttcgcc atcagctttt caacttgctg ataatcacct
     1321 tccgccagtt tcagcagcgc ctgttcggtc tgcttacgcg cacggcgacg cttacgtccg
     1381 acaaaccatc cacgggtgtg cgcgccagtg cggaagatcc gccgcagtag ccactcaatg
     1441 gcaaacagca ctaccatcgc cagaatcaat atgatcgcca ggcccgtgac gctggtttcg
     1501 atattgtagt tgtcggtctg gatcagcaca taaccctgat gaccggcaat catcgggcca
     1561 accactatcc ccgcaatcag caaaacaaag agtaataaca cttttagcat ggttattctc
     1621 cttgcggcgc ggctgccgga gtatcacctt gcggtgcagg tgcaggtgca ggtgcaggtg
     1681 caggtgcagg tgcaggttta gcttccgttg tccccgctgc cggttgtgcc agcaggttac
     1741 gcacgcgagt ctgcatcagt ttttccagca tcgcctggct ttgcagggtt tccggaagat
     1801 ccatcgagat attttgctgg cttaactggt ccacctcgtc gaggaacgct ttggtggtgg
     1861 catcatcagt atcgtagtaa gcacgtaccc aggtggagac gttctccagc gcctggcgat
     1921 aagtctcttc ctgatgacgc ggtacagctt gtgctgcgac cagcaggcga gagcgaatat
     1981 tttcgcgcag atagatatcc tgatttggcg ctaacagcgg tacggcggtg tcatcacgac
     2041 ggcgaatcgt aatgaagttg tccataaagt tctgccagct tttttgcaga ttgatacgcc
     2101 attcgctgat ggaactggaa agctcttcac cgtctgaatc catcggcgaa ccatcgctgt
     2161 cattatcggc cagacgcagg ttatctacct gatttgaaag ctgattaagc ttaaggatga
     2221 tgccgtcata atccacctgc gatactgcag aaaggctggc gatatcatcg gtaattgccc
     2281 gacgaacggt aatcagactc gggtcattca tatccgccag gctggcgtct gcacttttca
     2341 gcaacgctgc agaggtcgtg acgtcctgat cgctccacag cttccgtccg gcgagtttca
     2401 ccagaaaatc ggcctgagcc agcagccagg ttttagcatc gctgccggaa atggtggcga
     2461 tcttttgttg gacttcatcc aactgttttg ccagcgtttc ttgctgacga ttcgcctgct
     2521 taagctgcgc agcttgttgc ttaataatgc cttccagctc ggctttttgg ctctcctggg
     2581 ctttttgcaa tgccgtcagt tggttagcca gggcatcgct ggtggcggtc tgattgacgg
     2641 cctgttgttt accccagcca tacaaaccga cacccgccgc cagagcaata gcgatagcca
     2701 ccgcgctgag aatcaatgca gtattgttct tactcttttt ttctgttgcg acaggttgtg
     2761 acgtggtgtc cacggcctcc ctggtctctt caaccacggc ggaggttttt tcttgttccg
     2821 tcattatggc ttcctgttat gagagttatt gtaatgcccg taaaagcgca tcgttgtcag
     2881 cgttatcggc gaccttaatg tcttgccagc ccagttcccg ggcgagtttc gccaaacgct
     2941 cactgacgac caatagtcga cagtgtagta accagtgctc acgataccat tgtgggatca
     3001 gcgaccagag ttgctgcaac atttcaccgc tggtaacaac gaccatcgtc acctcgcggg
     3061 attgccagcg catcgcttct tctgcaccat cgtaatggat tgcgcatcgt tgataacatt
     3121 cacaaaaagt gacctcagca ccgcgcaccg tcagggtatc cccaattagc tcacgaccgc
     3181 cattgccacg taatatcagc gcacgtttgc ccgcaatatt ttgtaattca ggtaattgta
     3241 gcaagacttc gctgatttcc cgatcctgtg ggtagagaat cttctgtccg cttacggtat
     3301 gtagcgccag tgcggtggtg cgtccaatgg cgaaataagt aggtagttgg ggccatttac
     3361 gatcttgctg atgcagctgt gattgggcaa aagcaaccgc gtgttgcgag agggcaaaca
     3421 acagatcgct ctcccccagc gctgccagtt gatcagcaag ttgcggtaat tgtcgacccg
     3481 gagaaaactc aatcagcgga aaatgccagg ccacctgccc cagtgtgcgc agacggctca
     3541 ctaactcttc tccagcggga gacgggcggg tgaccaggat actcatgccg gggcctctcc
     3601 gttatagact tcagcgagga tctcgcgcgc gccgttattc agtagctctt ctgccagcga
     3661 aatccccatt tgttcggcat cttgcggcgc accgcggcgt tcaccgcgaa taatctgcga
     3721 accgtccggc gcgccgacca gcgcacatag ccagatttcg ccatcaataa gctcggcgta
     3781 gctaccaatt ggcacctgac atccgccttc gagacgggta ttcatggcgc gttctgcggt
     3841 aacgcgcagt gcagtttcgt ggtgattcag cgcggcaagc agctcgcgag tgcgtgtatc
     3901 atcaaggcgg cattcaatac cgactgcacc ttgtcctacc gccggaagag aaatctcggg
     3961 tggtaacgcg gcgcgaatgc gggactccag acctaaacgt tttagtccgg ctacggcaag
     4021 aatgatggca tcgtattcgc cgttatctag tttgctcagg cgagtgccga cgttgccgcg
     4081 cagggagcgg ataatcagat ccgggcggcg ttcagccagt tggcactggc gacgtaaact
     4141 ggacgtcccg acgatactgc ctgctggtaa cgcatccaga ctgtcatagt tattggacac
     4201 aaaggcatcg cgaggatctt cacgctcgca aatagtgacc agccccagac cttgcgggaa
     4261 ttcaaccggc acatctttca ttgaatgtac ggcgatatca gcgcgattct ccaggagcgc
     4321 gacttccagc tcttttacaa ataagccttt tccgcctact ttcgccagcg gcgtatcaag
     4381 aatcacatcg ccgcgcgtca ccatcggtac cagttcaacg accaggcccg gatggctcgc
     4441 catcaacttg tctttgacat agtgtgcctg ccagagtgca agtgggcttt ggcgtgtggc
     4501 aattcttaaa acattgtcta acatgcttgt taccgtcatt atcatccgtg gtccatccta
     4561 acatccttgc cagagtgatg tcagtgttgt ggtgaaacgt agacgcctgc gcaaaccgta
     4621 aaatgaggtc tggcagtgga tcctgacagg cgtttcacgc cgtcgtaata aggaatttac
     4681 agagaataaa cggtgctaca cttgtatgta gcgcatcttt ctttacggtc aatcagcaag
     4741 gtgttaaatt gatcacgttt tagaccattt tttcgtcgtg aaactaaaaa aaccaggcgc
     4801 gaaaagtggt aacggttacc tttgacatac gaaatatccc gaatgccgcg tgttaccgtt
     4861 gatgttggcg gaatcacagt catgacgggt agcaaatcag gcgatacgtc ttgtacctct
     4921 atattgagac tctgaaacag agactggatg ccataaatca attgcgtgtg gatcgcgcgc
     4981 ttgctgctat ggggcctgca ttccaacagg tctacagtct actgccgaca ttgttgcact
     5041 atcaccatcc gcgaatgccg ggttaccttg atggtaacgt tcccaaaggc atttgccttt
     5101 acacgcctga tgaaactcaa cgccactacc tgaacgagct tgaactgtat cgtggaatgt
     5161 cagtacagga tccaccgaaa ggtgagcttc caattactgg tgtatacacc atgggcagca
     5221 cctcgtccgt agggcaaagt tgttcctctg acctggatat ctgggtctgt catcaatcct
     5281 ggctcgatag cgaagagcgc cagttactac aacgtaaatg cagcctgctg gaaagctggg
     5341 ccgcctcgct gggtgtggaa gtcagcttct tcctgattga tgaaaaccgc ttccgtcata
     5401 atgaaagcgg cagcctgggg ggcgaagatt gtggctccac ccagcatata ctgctgcttg
     5461 acgaatttta tcgtactgcc gtgcgtctcg ccggtaagcg tattctgtgg aatatggtgc
     5521 cgtgcgacga agaagagcat tacgacgact atgtgatgac gctttacgcg cagggcgtgc
     5581 tgacgccaaa tgaatggctg gatctcggtg gcttaagctc gctttctgct gaagagtact
     5641 ttggtgccag cctttggcag ctctacaaga gtatcgattc cccatacaaa gcggtgctga
     5701 aaacactgct gctggaagcc tattcctggg aatacccgaa cccacgtctg ctggcgaaag
     5761 atatcaaaca gcgtttgcac gacggcgaga ttgtatcgtt tggcctcgat ccatactgca
     5821 tgatgctgga gcgtgttact gaatacctga cggcgattga agatttcacc cgtctggatt
     5881 tagtacgtcg ctgcttctat ttaaaagtgt gcgaaaaact cagccgtgaa cgcgcctgtg
     5941 taggctggcg tcgcgcagtg ttgagccagt tagtgagcga gtggggttgg gacgaagctc
     6001 gtctggcaat gctcgataac cgtgctaact ggaagattga tcaggtgcgt gaggcgcaca
     6061 acgagctgct cgacgcgatg atgcagagct accgtaatct gatccgcttt gcgcgtcgca
     6121 ataaccttag cgtctccgcc agtccgcagg atatcggcgt actgacgcgt aaactgtatg
     6181 ccgcgtttga agcattgcca ggtaaagtga cgctggtaaa cccgcagatt tcacccgatc
     6241 tctcggaacc gaatctgacc tttatttatg tgccgccggg ccgggctaac cgttcaggtt
     6301 ggtatctgta taaccgcgcg ccaaatattg agtcgatcat cagccatcag ccgctggaat
     6361 ataaccgtta cctgaataaa ctggtggcgt gggcatggtt taacggcctg ctgacctcgc
     6421 gcacccgttt gtatattaaa ggtaacggca ttgtcgattt gcctaagttg caggagatgg
     6481 tcgccgacgt gtcgcaccat ttcccgctgc gcttacctgc accgacaccg aaggcgctct
     6541 acagcccgtg tgagattcgc catctggcga ttatcgttaa cctggaatat gacccgacag
     6601 ctgcgttccg caatcaggtg gtgcattttg atttccgtaa gctggatgtc ttcagctttg
     6661 gcgagaatca aaattgcctg gtaggtagcg ttgacctgct gtaccgcaac tcgtggaacg
     6721 aagtgcgtac gctgcacttc aacggcgagc aatcgatgat cgaagccctg aaaactattc
     6781 tcggcaaaat gcatcaggac gccgcaccgc cagatagcgt ggaagtcttc tgttatagcc
     6841 agcatctgcg cggcttaatc cgtactcgcg tgcagcaact ggtttctgag tgtattgaac
     6901 tgcgcctttc cagcacccgc caggaaaccg ggcgtttcaa ggcgctgcgc gtttctggtc
     6961 aaacctgggg cttgttcttc gaacgcctga atgtatcggt acagaagctg gagaacgcca
     7021 tcgagtttta tggcgctatt tcgcataaca aactgcacgg cttgtcagtg caggttgaaa
     7081 ccaatcacgt caaattaccg gcggtggtgg acggctttgc cagcgaaggg atcatccagt
     7141 tctttttcga agaaacgcaa gacgagaatg gctttaatat ctacattctc gacgaaagca
     7201 accgggttga ggtgtatcac cactgcgaag gcagcaaaga ggagctggta cgtgacgtca
     7261 gtcgcttcta ctcgtcatcg cacgaccgct tcacctacgg ctcaagcttc atcaacttca
     7321 acctgccgca gttctatcag attgtgaagg ttgatggtcg cgaacaggtg attccgttcc
     7381 gaacaaaatc gattggcaac ctgccgcccg ccaatcagga tcacgatacg ccgctattac
     7441 agcagtattt ttcgtgatga gcgtgccgga aagcgaggct tatccggcat gcaatcttag
     7501 cggaaactga ctgtttcacc cgcctgttgc gtcgccgcct gttccagcaa atcccagaag
     7561 gtttcgccgc tgcgatcgca aatccattca tcgcctttca ggtcaaaatg gtagccgccc
     7621 tgtttggttg ccagccacac ctggtgcagc ggctcctggc ggttaataat gattttgctg
     7681 ccgttttcaa aggtaatggt cagtacgccg ccgttgattt cgcagtcgat atcgctgtcg
     7741 ccatccctgt cgtccagacg ctcttcaatg gtcagccaca gttgatcagc caggcgatga
     7801 aattcactgt cgttcattgt tgtatcctgt ttttaagtga tggcggcagt atagcggcat
     7861 ggggtcaggg cttcaaagtt tgcacctctg ctgctgcgtt ccggcatgat tcatccgtca
     7921 ccggaataat gatgtctctg ttttgaactt cacgtacgtt cccctctctg acacgagttt
     7981 tcgccgggca gggggagggc gtaatcctta cctctgtatc ggtccatgta taaacattgc
     8041 gatcgtcata agcgaagcga tcgtttttag ttagcgaacc gttaactgga ataatttgca
     8101 agctctttga ccgtaattgg cggataaagt aagcatgctg tttgccgcta ttaaatgagc
     8161 gatcaatgac caggtctggc gtatccagcg ggattatctc aagctggtct tcaccaaatg
     8221 tcgataattt tgttacatac agccgatctt tatattgcgc aatattgcca gtaatcagat
     8281 ggaatt
//
LOCUS       DAZPCS010000156         8253 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZPCS010000156 DAZPCS010000000
VERSION     DAZPCS010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8253
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.157"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..395
                     /locus_tag="ACHUQ7_003350"
     CDS             <1..395
                     /locus_tag="ACHUQ7_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1136623.1"
                     /translation="TDDMIKANAWYLYKEWIRSDDVSPIFIETEEKLRTFNTNKLTRN
                     DNIFILFSSVDDGPVMVVSSQRLHDMLNPTKDTNWNSTCIYKSRHKMLPINLTQETLF
                     SSKSHGKYALFPIFTASWRATRIKNKGI"
     gene            618..1115
                     /gene="ubiC"
                     /locus_tag="ACHUQ7_003351"
     CDS             618..1115
                     /gene="ubiC"
                     /locus_tag="ACHUQ7_003351"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFR1136624.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            1128..2000
                     /gene="ubiA"
                     /locus_tag="ACHUQ7_003352"
     CDS             1128..2000
                     /gene="ubiA"
                     /locus_tag="ACHUQ7_003352"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFR1136625.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWIMAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(2155..4578)
                     /gene="plsB"
                     /locus_tag="ACHUQ7_003353"
     CDS             complement(2155..4578)
                     /gene="plsB"
                     /locus_tag="ACHUQ7_003353"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFR1136626.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4749..5117
                     /gene="dgkA"
                     /locus_tag="ACHUQ7_003354"
     CDS             4749..5117
                     /gene="dgkA"
                     /locus_tag="ACHUQ7_003354"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFR1136627.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5227..5835
                     /gene="lexA"
                     /locus_tag="ACHUQ7_003355"
     CDS             5227..5835
                     /gene="lexA"
                     /locus_tag="ACHUQ7_003355"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFR1136628.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5908..7233
                     /gene="dinF"
                     /locus_tag="ACHUQ7_003356"
     CDS             5908..7233
                     /gene="dinF"
                     /locus_tag="ACHUQ7_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFR1136629.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7349..7558
                     /locus_tag="ACHUQ7_003357"
     CDS             7349..7558
                     /locus_tag="ACHUQ7_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFR1136630.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7600..8115)
                     /gene="zur"
                     /locus_tag="ACHUQ7_003358"
     CDS             complement(7600..8115)
                     /gene="zur"
                     /locus_tag="ACHUQ7_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFR1136631.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
BASE COUNT         1871 a         2004 c         2251 g         2127 t
ORIGIN      
        1 caacggatga tatgatcaaa gcaaatgcct ggtatcttta taaagagtgg attcgtagtg
       61 atgatgtttc acctatattt atagaaactg aagaaaagtt aagaaccttt aacactaata
      121 aattaacacg aaacgataat atatttatcc tgttctcctc tgtcgatgat gggcctgtta
      181 tggtggtaag ctcccagcgc ttacatgata tgttgaatcc tacaaaagat accaattgga
      241 attccacgtg tatctataaa tccagacata agatgttgcc tattaatctt actcaggaaa
      301 cacttttcag ctccaaatct catggtaaat atgcgctttt cccaattttt actgcgagtt
      361 ggcgagctac tcgtataaag aataaaggta tttaagtaaa ggaaaacatc accgttccta
      421 gcatcatggg cggtgatgcc cctatggttg ccctcgccag cacgggcatc ggcaaagcgt
      481 aaggttcaac agcgttttac cacttcatgc gattgttgcg tttttgttgc gcattagatc
      541 acttaatttg ctttacatct cccgtaaaca cttttctgcg atacaatgcc tttacgttat
      601 gtaacggaga gttcggcatg tcacaccccg cgttaacgca actgcgtgcg ctgcgctatt
      661 ttaaagagat ccctgccctg gatccgcaac tgctcgactg gctgttgctg gaggattcca
      721 tgacaaaacg ttttgaacag cagggaaaaa cggtgagtgt gacgatgatc cgcgaggggt
      781 ttgtcgagca gaatgaaatc cccgaagaac tgccgctgct gccgaaagag tctcgttact
      841 ggttacgtga aattttgtta tgtgccgatg gtgaaccgtg gcttgccggt cgtaccgtcg
      901 ttcctgtatc aacattaagc gggccggaac tagcgttaca aaaattgggt aaaacgccgt
      961 taggacgcta tctgttcaca tcatcgacac taacccggga ttttatcgag ataggccgtg
     1021 atgccggact gtgggggcga cgttcccgcc tgcgattaag cggtaaaccg ctgttgctaa
     1081 cagaactgtt tttaccggcg tcaccgttgt actaagagga aaaaaatatg gagtggagtc
     1141 tgacgcagaa taagctgctg gcgtttcatc gcttaatgcg tacggataag ccaattggcg
     1201 cgttactgct gctctggcca acattatggg cgttgtgggt ggcgacaccg ggcgttcctc
     1261 agctctggat catggcggtg tttgtcgcgg gtgtctggct gatgcgcgct gctggttgcg
     1321 tggtgaatga ttatgctgat cgcaagtttg atggtcatgt taagcgcacg gcgaaccgac
     1381 cacttcccag cggcgcggta acagagaaag aggcgcgcgc gctgtttgta gtgctggtac
     1441 tgatttcgtt tttactggtt ctgacgctga atacgatgac cattctgttg tcgattgccg
     1501 ctctggcatt ggcgtgggtg tacccgttta tgaagcggta tacccatctc ccgcaagtgg
     1561 tgctgggcgc ggcgtttggc tggtcgattc caatggcttt tgccgctgtg agtgagtcgg
     1621 tgccattgag ttgctggtta atgttcctcg ccaatattct ctgggcggtg gcttacgaca
     1681 cgcagtatgc gatggttgac cgcgatgatg atgtgaagat tggcattaaa tccacggcaa
     1741 tcctgttcgg ccaatacgat aaattgatta ttggtatttt gcagattggc gtactggcac
     1801 tgatggcgat catcggtgag ttaaatggct taggctgggg atattactgg tcaattgtgg
     1861 tggctggcgc gctgtttgtt tatcaacaaa aactgattgc caaccgcgag cgtgaagcct
     1921 gctttaaagc atttatgaat aataactatg ttggtctggt actgttttta ggactggcaa
     1981 tgagttactg gcatttctga tgatgtaaaa aagccggatg atcatccggc tttcttctgg
     2041 gttgcctgat gcgttgcgct tctcaggcct acccggtgaa tcgcaattta ttgaatttgc
     2101 aggtcatggt aggccggata aggcgttttc gccgcatccg gcaattctct ctgattaccc
     2161 ttcgccctgc gtcgcactct caatcgtcaa acgcacgtct gatgtaatca actccgccag
     2221 caactgataa accttcatcg tttctgctgg ttcggcatcg ccgctatcgc tgatataccc
     2281 ttcatcacgc agcgtcagca ccagagaact gaacaccgcc ttgtcgaaga actccggcgc
     2341 gttgatgccg tgcagcacgg agagacgttg cgcaacggtg cggctctctt tctccagcgt
     2401 accgcggttg atcgacgggt tggcactcaa caaccagaag gtgatggcat aacgttgcag
     2461 cgtttcgcgc gcgcctgcgg ccagcagctg tagggtgcga gaatgcgccg ggttgatatg
     2521 caactcatca tcttgcaggg taatcagccc ctgacgttgc atctcatttg ccagcgcatc
     2581 aataacgtcc ggcaactcgt cgcgatccca gcgcaggaac agctccgctt tcagcattgg
     2641 gtaaagcaca ttgacgtgct ccatcaatac gtcgcgggag atgtggcgat gctgggtgac
     2701 aattgccgcc atcagcgaag gcagcaccaa catatgcgca atgttgttgc gatagtaggt
     2761 catcagcacc gcttgctcgc gtggcagaat gatgatgtcg ccgatagtgt ctttctcgac
     2821 ttcaaacttg ttcatttgca gcgcgtgatc gataagctcg ctggcgctgg ctgaaggaac
     2881 ggtagagtcc gtggagtaag gtacgttgcg catcagatcc aggtagcagt tgagttgctc
     2941 ggttaactgc tcgcgggtga gtgagcgctg acgtgatgcc agtagcgcag tacagcacag
     3001 gttcatggcg tttgccgcgc ctgcgttgtt aatgcgtacc atcagatcgg cagcaatatt
     3061 attgaccgtc ggcgttaacc aggccggacg caccgcttcg atgggatcga tagattcacg
     3121 ccagtctggt acgtgctggt taaggtaggt catcaacggc attggttcac cgaagttgac
     3181 gtaaccctga ccgagattac gcagcttgct taaaccgcgc agcatctgcg gcaggctctc
     3241 tttctctttc gtcgcgccgc gcagttcttt ggcgtaagta cccacttcca tgacgtgctc
     3301 ataaccgata tagatcggaa tcagcgtaat cggacgcgtg ccgccacgca gcatcgcctg
     3361 aatggtcatc gacagcgtac cggttttcgg atccagcaaa cgccccgtgc gggaacgacc
     3421 gccttccacg aagtactcga cggaataacc acggctgaac agttcgccga gatactcacg
     3481 gaaaacggtg gaataaagtt tattgccttt aaaagtacgg cgaataaaga acgcccccag
     3541 acggcggaaa atcggcccgg ccggccagaa gttcaggttg atcccggcgg cgatatgcgg
     3601 cggcaccagt ccctggtggt acagcacgta agaaagtagc aggtagtcca tgtgactgcg
     3661 gtggcaaggc acatacacca gctcatgacc gtcgtgggcc agctggcgaa cgcgctcggc
     3721 gttatggacg ttgatgccct gataaaatcg gttccaggtg aagcccagaa tgcggtcagt
     3781 caggcggatc atctcgtaag agaaattcgc cgcaatctct tccatcagcg caatcgcgtt
     3841 ctgctgcgct ttttcatggg agattttttt gctgcgcgct tcatcttcta ccgctttggc
     3901 aatggcgcgg gaggcgagca gcttattaaa cagatcctga cgagcaggca gacgtgggcc
     3961 tacggcagcc agacgttgac gggcaaagtg catacgcgcc acgcgcgcca gtttctgagc
     4021 gatagtttta tccgtgccgt gttcatccgc catacggcgc agcgacactg acggcgagaa
     4081 acgcacaaaa ctgtcgcgac cgagccacag tacagcgaaa aatttctgta cgccgttaag
     4141 catacgcagc ggcgggttca cttcgccttt ttcacgcccc ggcgcgcgac caaacatcac
     4201 cgatactggc accatctgca catccagatt tgggttgcta cggtgcaaat cgagatagtc
     4261 gtggaaaagc ttaatagact cttctttcgg cgtgtaatag gtgaatacac gcggcccgcc
     4321 gtgaatgaac acatagcgcg gcagtagcgt gccgtcgatt tccagcggct ctaacgggtc
     4381 aggcaaatca tgtgccagac actgggcgcg caacgtcagc aaatctgctt tcgagttgta
     4441 cggtaaaacg tacataattg gacgagaggt atccagcccc agttccgggg caggatctgc
     4501 cggaatagac ttgcttttta ccaggatgct taatggtaaa ttcagtaatt tgtagtaaat
     4561 tcgtggccag ccggacataa acgatgtaaa gcctctggtt aataatgcaa atgcgcggca
     4621 aggatagcag aaagtcatgg gaaattctgt ggtatccgct catgtttcgc gcggcgttac
     4681 gcaaacccga atcatcggat ttaacggtac actgatattg acgctcataa tgtaaaaagg
     4741 ttctttcaat ggccaataat acaactggat tcacccgaat tatcaaagct gctggctatt
     4801 cctggaaagg tttacgcgct gcatggatca acgaagcggc attccgtcag gaaggcgtag
     4861 cggtattgtt ggcggtggtc atcgcctgct ggctggatgt ggacgcaatt acccgcgtgc
     4921 tgcttatcag ctccgtgatg ctggtgatga ttgtggaaat cctcaatagc gccatcgaag
     4981 cagtggttga ccgaattggc tctgaatacc atgagctttc cggacgcgca aaagatatgg
     5041 gatccgccgc ggtgctgatt gccattatcg tcgccgtgat tacctggtgc atcctgttat
     5101 ggtcgcattt tggataaccc ttccagattt cgataaatct ctggtttatt gtgcagttta
     5161 tggttccaaa atcgcctttt gctgtatata ctcacagtat aactgtatat acacccaggg
     5221 ggcggaatga aagcgttaac ggccaggcaa caagaggtgt ttgatctcat ccgtgatcac
     5281 atcagccaga caggtatgcc gccgacgcgt gcggaaatcg cgcagcgttt ggggttccgt
     5341 tccccaaacg cggctgaaga acatctgaag gcgctggcac gcaaaggcgt tattgaaatt
     5401 gtttccggcg catcacgcgg gattcgtctg ttgcaggaag aggaagaagg gttgccgctg
     5461 gtaggtcgtg tggctgccgg tgaaccgctt ctggcgcaac agcatattga aggtcattat
     5521 caggtcgatc cttccttgtt caagccgaat gctgatttcc tgctgcgcgt cagcgggatg
     5581 tcgatgaaag atatcggcat tatggatggc gacttgctgg cagtgcataa aactcaggat
     5641 gtacgtaacg gtcaggtcgt tgtcgcacgt attgatgacg aagttaccgt taagcgcctg
     5701 aaaaaacagg gcaataaagt cgaactgttg ccagaaaata gcgagtttaa accaattgtc
     5761 gttgaccttc gtcagcagag cttcaccatt gaagggctgg cggttggggt tattcgcaac
     5821 ggcgactggc tgtaacatat ctctgagacc gcgatgccgc ttggcgtcgc ggtttgtttt
     5881 tcatctctct tcatcaggct tgtctgcatg gcattcctca cttcatctga taaagcactc
     5941 tggcatctcg ccttacccat gattttctcc aatatcaccg ttccgttgct gggactggtc
     6001 gatacggcgg taattggtca tcttgatagt ccggtttatt tgggcggcgt ggcggttggt
     6061 gcaacggcga ccagctttct ctttatgctg ttgctgtttt tacgcatgag caccaccggg
     6121 ctgactgcgc aggcttatgg tgccaaaaat cctcaggcat tagcccgtgc gctggtgcaa
     6181 ccgttgctgt tggcgttggg ggccggggcg ttaattgcgc tgttgcgtac gccgattatc
     6241 gatctggcgc tgcatattgt tggcggtagt gaagcggtcc tggaacaggc gcggcgcttt
     6301 cttgaaatcc gctggttaag cgctccggcg tcgctggcga atctggtatt actcggttgg
     6361 ttactcggcg tgcaatatgc ccgtgcgcca gtaattttgt tagtggtcgg caatatcctc
     6421 aacattgtgc tggatgtctg gctggtgatg gggctgcata tgaacgtgca gggcgcggcg
     6481 ctggcgacgg ttattgcgga atatgcaaca ttgctgattg gtctgctaat ggtgcgtaaa
     6541 atcctcaaac tacgcggaat ttccggcgaa atgctgaaaa ctgcctggcg aggaaacttc
     6601 cgtcgcttgc tggcgcttaa ccgcgatatc atgctgcgtt cgctgttgtt gcaactctgt
     6661 ttcggcgcga tcaccgtact tggtgcgcga ctggggagtg acattatcgc tgttaacgcg
     6721 gttctgatga cgctactcac ctttaccgcc tatgcgcttg atggttttgc ctacgcggtt
     6781 gaagcgcact ccggtcaggc atacggtgcg cgcgacggta gccagttgct ggatgtatgg
     6841 cgggcagcgt gccgccagtc ggggattgta gcgttactat tttcggtggt ttatttgctg
     6901 gcaggggaac acatcattgc gttgctgacg tcgttaaccc agattcagca gctggctgac
     6961 cgctatctta tctggcaggt gattttgccg ttggttggcg tctggtgtta tctgctggac
     7021 ggcatgttta taggcgcaac gcgtgccgcc gaaatgcgta acagtatggc ggtggccgcc
     7081 gcaggttttg cgctgacgct ccttacgctg ccgtggctgg gtaatcatgg tttgtggctg
     7141 gcattaaccg tctttctggc gttgcgcggg ctttctctgg cggctatctg gcggcgtcac
     7201 tggcgcaacg gtacctggtt tgccgcaacg tgacggttaa aaattctgaa taaattatcc
     7261 taagccaaat tgctgactac acttaatctc acgttcagaa gaaaagtgaa cgtactctca
     7321 ttcacaacct aacgatgagg tcttgattat gaataaagat gaagccggcg gtaactggaa
     7381 acagtttaaa ggtaaagtga aagagcaatg gggcaaactg accgatgatg atatgacgat
     7441 cattgaaggt aaacgtgatc aactggtcgg taaaatccag gaacgttatg gttatcagaa
     7501 agatcaggca gaaaaagagg tcgtggattg ggaaacccgc aatgaatatc gctggtaatt
     7561 aatccctcct gcccgacgtg tacaaggatg tacgccctct taacgcggtt tctttttcac
     7621 ctgcacagag tgatcatgct ggcactgttc aggatgacga cacgcttcca cttccacgca
     7681 tgccgcacag agcccatgtg cttcaatcac attatgccgc agggcaaacc ccatttttgc
     7741 cgccagcgta tgcataatgt cttccacgcc ttctgcacac tcttctttca ccgcgccgca
     7801 gcgatcgcaa ataaacatgg ctgacgtatg cgtgggctgg tcgaacagat gacagagcac
     7861 ataactgttg gtggattcca ccttatgcac aaaaccttgc tcaagcagaa aatccagcgc
     7921 gcgataaacc gttggcggct tggcttgcgg ttcagcttcg cgcagtaaat caagcagatc
     7981 ataagcgctg atagcgccat cctgcagact catcaggcgc aacacttcca ggcgctgtgg
     8041 ggtcaggcgc acattacgct gcgcgcagat tttttcagcc tgcgctaata actcctgcgt
     8101 tgtggtcttt tccatcgggc acctcaaagt gggggttaag aatgtcctta ctttaccatg
     8161 ttccaggaaa aacaccgaga acccttattg ttgccgtaat gttgattttc tgttttgtag
     8221 gtaaggtgtt gtgttgcctt atcgtaccat tat
//
LOCUS       DAZPCS010000157         8147 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZPCS010000157 DAZPCS010000000
VERSION     DAZPCS010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8147
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.158"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            94..1590
                     /gene="ravA"
                     /locus_tag="ACHUQ7_003359"
     CDS             94..1590
                     /gene="ravA"
                     /locus_tag="ACHUQ7_003359"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFR1136632.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFILRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEDSLQDVGAQIRQAQQC"
     gene            1584..3035
                     /gene="viaA"
                     /locus_tag="ACHUQ7_003360"
     CDS             1584..3035
                     /gene="viaA"
                     /locus_tag="ACHUQ7_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFR1136633.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQFIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVFERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="ACHUQ7_003361"
     CDS             complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="ACHUQ7_003361"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFR1136634.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQLSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            4184..4642
                     /gene="asnC"
                     /locus_tag="ACHUQ7_003362"
     CDS             4184..4642
                     /gene="asnC"
                     /locus_tag="ACHUQ7_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFR1136635.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4732..5175
                     /gene="mioC"
                     /locus_tag="ACHUQ7_003363"
     CDS             4732..5175
                     /gene="mioC"
                     /locus_tag="ACHUQ7_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFR1136636.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEVGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGTIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            5554..7443
                     /gene="mnmG"
                     /locus_tag="ACHUQ7_003364"
     CDS             5554..7443
                     /gene="mnmG"
                     /locus_tag="ACHUQ7_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFR1136637.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQISGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            7507..8130
                     /gene="rsmG"
                     /locus_tag="ACHUQ7_003365"
     CDS             7507..8130
                     /gene="rsmG"
                     /locus_tag="ACHUQ7_003365"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFR1136638.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
BASE COUNT         2044 a         2089 c         2075 g         1939 t
ORIGIN      
        1 attacccccc tttgagacta ttttagtcac cagcaagaaa cgtctatact cgcaatttac
       61 gcagaacttt tgacgaaagg acgccacttc attatggctc accctcattt attagcggaa
      121 agaatttccc gcctgagcag ttcgttggaa aaggggcttt atgaacgtag ccacgccatc
      181 cgcttgtgtt tattagcggc attaagtggt gaaagtgtgt tcctccttgg cccgccaggt
      241 attgccaaaa gtttgatcgc ccggcgctta aaattcgcct ttcagaatgc ccgcgcgttt
      301 gaatatctga tgacccgctt ctccacgccg gaagaagttt ttggtcccct ttctattcag
      361 gcgctaaaag atgaagggcg ctatgaacgt ttaaccagcg gttacctgcc ggaagcagaa
      421 atcgtctttc tggatgagat ctggaaagcg ggcccggcaa ttcttaatac cttgctcacc
      481 gccattaacg agcgccagtt ccgcaacggt gcacacgtag aaaaaatccc gatgcgtcta
      541 ctggtggcgg cctccaacga gctgccggaa gcagacagca gtctggaagc gttatatgac
      601 cgcatgctga ttcgtctatg gttagataaa gtgcaagata aagcgaattt ccgctccatg
      661 ctgaccagtc aacaggatga aaacgacaat ccggttcctg atgccttgca ggtcacagat
      721 gaagaatatg aacgctggca gaaagagatt ggtgaaatta cgctgcccga tcatgtattt
      781 gagctgattt ttattctgcg ccagcaactg gataaattac cggatgcgcc ttatgtctcg
      841 gatcgtcgct ggaaaaaagc gatccgctta ttgcaggcca gcgccttttt tagcggtcgc
      901 agtgctgttg ccccggttga tctcattttg ctgaaagatt gcctgtggta tgacgcgcaa
      961 agcctgaatt tgatacaaca acaaattgat gtattgatga ccggtcatgc ctggcaacag
     1021 caagggatgt tgacccgcct gggcgcgatt gtgcaacgtc acctgcaact ccagcagcaa
     1081 caaagcgata aaacagcctt aacgttaatt cgtctgggcg gcattttcag ccgtcgtcag
     1141 cagtatcaac ttcctgttaa cgttactgct tccactctga ctctgctgct gcaaaaaccg
     1201 ttaaaactgc atgatatgga agtggttcat atctcctttg agcgtagcgc gctggaacag
     1261 tggctgagta aaggtggtga aattcgcggc aaactgaacg gtatcggctt tgcccagaaa
     1321 ctgaatctgg aagttgatag cacccaacat cttgttgtac gcgatgtaag tttacaaggc
     1381 agtacgctgg cacttcccgg ttcatcggct gaaggtctgc caggtgaaat aaaacaacaa
     1441 ctggaagagc ttgaaagcga ctggcgtaag caacacgctt tattcagcga acagcaaaaa
     1501 tgtctgttta tccctggcga ctggttaggt cgcattgaag acagcctaca ggatgtcggt
     1561 gcacagattc gtcaggcaca acaatgctaa cgctggatac gcttaatgtg atgctggccg
     1621 tcagcgaaga gggattgatc gaagagatga tcatcgcact gctggcctca ccgcagctgg
     1681 cggtcttctt tgaaaaattc ccacgactga aggcggcaat cactgatgat gttccccgct
     1741 ggcgtgaggc gctgcgcagt cggctgaaag atgcccgagt cccgccggaa ctcaccgaag
     1801 aggtgatgtg ctatcagcaa agccagctcc tctccacgcc gcagtttatt gtgcagctac
     1861 cacagatcct ggacttactg catcgtctga attccccatg ggcagaacaa gcccggcagt
     1921 tggttgatgc taacagcaca ataacttcag cgttacacac gctttttctc cagcgttggc
     1981 gtttaagtct gatcgtgcaa gcaacgacgt taaatcaaca gctattagaa gaagaacgcg
     2041 aacaactgtt aagtgaagtt caggaacgca tgacgctgag cggacaactt gaaccgattc
     2101 tcgcagataa caataccgca gctggtcgtc tgtgggatat gagcgccggt caacttaaac
     2161 gtggcgacta tcagtttatt gtgaaatacg gtgaatttct taacgaacag ccggaactga
     2221 aacgcctggc agagcagctg gggcgttctc gggaagccaa atcaataccg cgcaacgatg
     2281 cgcagatgga aaccttccgc accatggtgc gcgaaccggc gacggttcct gagcaggttg
     2341 atggtctgca acaaagcgat gatattttac gtctcctgcc gccagaactg gcgacactag
     2401 ggataacgga actggagtat gagttttacc gtcggctggt ggaaaaacag ttgctcacct
     2461 atcgcctgca cggtgagtcg tggcgtgaaa aagtgttcga acgtccggtg gtacataaag
     2521 attacgatga acagccgcgc gggccgttta ttgtctgtgt ggatacttcc ggctcaatgg
     2581 gcggctttaa tgaacagtgt gcgaaagcgt tctgcctggc tttgatgcgc attgctctcg
     2641 ctgaaaaccg gcgctgttat attatgctat tttccaccga gatcgtccgt tatgagcttt
     2701 caggcccaca aggcatcgaa caagcaatcc gttttttaag ccagcagttt cgtggcggca
     2761 ccgatcttgc cagttgtttt cgcgccatta tggaacgctt gcaaagcagg gaatggtttg
     2821 atgccgatgc ggtggtgatt tctgatttta tcgctcagcg gttgcctgac gacgtgacga
     2881 gtaaagtgaa agagctgcag cgggtacatc agcatcgctt tcatgccgtg gcgatgtcgg
     2941 cacacggcaa acccggcatc atgcgcattt tcgatcatat ctggcgcttt gataccggga
     3001 tgcgaagccg cctgctcaga cgctggcggc gataaattat tacagcagag aagggacgct
     3061 ctcgcgaaca gcagctggcc atactccaca ctgaacctgg ccgatatgcg gcagttgcag
     3121 cagcagcata gtcaaacgag actggccgat accgccgccg atggtctgcg gcatttcacc
     3181 gcgcagcagc gcctgatgcc attccagctg cagacgatct tcatcaccgg tcagcgccag
     3241 ctgatgcttc agcgtgtcgg catccacgcg gatccccatg gaagaaagct caaacgcatc
     3301 ttccagtacc gggttccaca ccagaatatc gccgttcagg cccgcatggc ccagctctga
     3361 cggggtgctc cagtcatcat aatccggtgc gcgcacgtcg tgacgatgac catcactcaa
     3421 cttgccgcca atcccgacga ggaataccgc gccaagatct ttcgcaatcg cccgctcacg
     3481 ccctttggca tcgagatccg gataacgaga cagtaactcc tggctgtgta cgaagtgaat
     3541 ctgatccggc aggaacggtg ccaggccaaa ctcttcgtta accgcagctt cggttgcttt
     3601 aattcccgcc cagatcgcct ctaccgtgct tttcagagtc gagagttgac gctcaccgtc
     3661 gcccattacg cgttcccagt cccactggtc aacatagacc gagtgcaacg gagaaagacg
     3721 gtcttcatcg gggcgaaggg ctttcatgtg cgtgtacagc ccttcgcccg cgctgaagtc
     3781 gtgttgccct aaggtctgac gtttccactt cgccagtgaa tgaaccactt cgaactgggc
     3841 atcaggcaga gctttcactt ttacctgcac cgctttttca cagcccgaca agttatcctg
     3901 cgtgccatcc cccacacggc taagaatcgg cgcctggact tcgatcagcc ccagacgttc
     3961 ttccagttgg cgagaaaagt gagatttcac gaagctaatt tggcgttgtt tggcaatgta
     4021 agcggttttc attttttata ctcctgcgtc ctgttgctta tgattaagca acaaaaaccg
     4081 acacacatgc aataatcatt caataaaaag cctaacacac ttttgattca ttaaaaaaga
     4141 cggctaaaat agaatgaatc atcaatctgc ataagaaaat cctatggaaa attatctgat
     4201 cgacaatctg gaccgtggca tcctggaagc attaatgggc aatgcgcgca ccgcttacgc
     4261 cgaactggcg aaacaatttg gcgtcagtcc ggggacgatt cacgttcgag tagagaaaat
     4321 gaagcaggcg gggatcatta ccggggcgcg tattgatgtc agcccgaagc agctcggtta
     4381 tgacgtaggc tgctttatcg gcattatatt aaagagcgcc aaagactacc cttccgcgct
     4441 ggcaaagctg gaaagccttg atgaagtcac tgaagcctat tacacaaccg gccactacag
     4501 catctttata aaagttatgt gccgttcgat cgacgctctc cagcatgtac ttatcaacaa
     4561 gatccaaaca attgatgaaa ttcagtccac cgagacattg atcgtcctgc aaaacccgat
     4621 catgcgtacc atcaagccct gatcggcttt tttaatccca tacttttcca caggtagatc
     4681 ccaacgcgtt cacagcgtac aatacgccac tcttaataaa ggtggcggtt tatggcagat
     4741 atcactctta tcagcggcag caccctcggc ggtgccgaat atgtagcaga acacctggct
     4801 gaaaagctgg aagaggtggg ttttaccacc gaaacgctgc acggtccgct gttggaagat
     4861 ttatctgcct cggggatctg gctggttatc agctccaccc acggtgccgg agatattccg
     4921 gacaaccttt ctcctttcta tgaagcattg caggaacaga agcccgatct ttctgcggtc
     4981 cgctttggca caatcggtat tggcagtcgt gaatatgaca ccttttgtgg ggctatcgat
     5041 aaactcgagg cagaactcaa aaattccggt gcaaaacaga caggcgaaac actgaagatc
     5101 aacattcttg atcacgacat tccggaagat ccggcagaag aatggctggg atcgtggatt
     5161 aatttactca aataagtata cagatcgtgc gatctactgt ggataactct gtcaggaagc
     5221 ttggatcaac cggtagctat ccaaagaaca accgttgttc agtttttgag ttgtgtataa
     5281 cccctcattc tgatcccagc ttatacggtc caggatcacc gatcattcac agttaatgat
     5341 cctttccagg ttgttgatct taaaaatcgg atccttgtta tccacagggc agtgcgatcc
     5401 taataagaga tcacaataga acagatctct aaataaatag atcttctttt taatacccag
     5461 gatcccaggt ctttctcaag ccgacaaagt tgagtagaat ccacggcccg ggcttcaatc
     5521 cattttcata ccgcgttatg cgaggcaatc accatgtttt atccggatcc ttttgacgtc
     5581 atcatcattg gcgggggtca tgcaggcacc gaggccgcga tggctgcggc gcgtatgggt
     5641 caacagactc tgcttttgac acacaatatc gacactctgg ggcagatgag ctgcaacccg
     5701 gcgatcggcg gtattgggaa gggacatctg gtaaaagaag tggatgcact cggcggtctg
     5761 atggcgaaag cgatcgatca agcgggtatc cagtttagga tactaaacgc aagcaaagga
     5821 ccggcggttc gcgctacccg agctcaggcg gatcgtgtgc gctaccgtca ggcggtacgt
     5881 acggcgctgg agaaccaacc gaacctgatg atcttccagc aggcggttga agatcttatt
     5941 gtcgaaaacg atcgcgtggt cggtgctgtt acccaaatgg gactgaagtt ccgtgccaaa
     6001 gctgtggtgc tcaccgttgg gacgttcctc gacggcaaaa ttcatatcgg tctggataat
     6061 tacagcggtg gccgtgctgg cgatccgcca tccattccgc tctctcgccg tttgcgtgaa
     6121 ctgccgctgc gcgttggtcg tctgaaaacc ggtactccgc cgcgtattga tgctcgaacc
     6181 atcgacttta gcgtgctggc gcaacagcat ggcgataacc caatgccggt attctcgttt
     6241 atgggcaatg cgtccctgca tccccagcag gtgccgtgtt atatcactca taccaacgag
     6301 aaaacccatg atgtgatccg cagtaacctc gatcgtagcc caatgtatgc aggggtgatc
     6361 gaaggtgtcg gcccacgcta ctgcccgtcg atcgaagaca aagtcatgcg cttcgcagac
     6421 agaaatcagc atcagatctt ccttgaaccg gaaggactga cctctaatga aatttatccg
     6481 aacggtatct ccaccagcct gccgttcgat gtgcagatgc aaatcgtccg ctctatgcag
     6541 gggatggaaa acgcgaagat cgtgcgtccg ggttatgcca ttgagtatga cttcttcgat
     6601 cctcgcgacc tgaaaccgac gctggagagc aagtttatcc aggggctgtt ctttgctggt
     6661 cagattaacg gcactaccgg ttacgaagaa gccgctgcgc aaggtttgct ggccggtctt
     6721 aacgctgccc gtctgtctgc tgacaaagaa ggttgggctc cggcacgttc tcaggcgtat
     6781 ctcggcgtac tggttgatga cctgtgcact ttaggaacca aagaaccgta tcgtatgttt
     6841 acttcgcgcg cagaatatcg tctgatgctg cgcgaagata atgcggatct gcgtttgact
     6901 gaaatcggtc gtgaactggg cctggtggat gacgaacgtt gggcgcgctt taacgagaaa
     6961 cttgagaata tcgagcgtga gcgtcagcgt ctgaaatcga cctgggtaac cccgtcggcg
     7021 gaagctgcag ccgaagtgaa tgctcacctg actgcgccgc tttcccgtga agccagtggt
     7081 gaagatctgc tgcgtcgtcc ggaaatgact tatgaaaaat taaccacgct gacgccgttt
     7141 gcccctgcgt tgacagacga acaggcggcg gaacaggttg agattcaggt taaatacgaa
     7201 ggttatatcg cgcgccagca agatgagatc gaaaagcagc tgcgtaacga gaacaccctg
     7261 ctacccgcga cactggatta ccgccagata tccggtcttt ctaacgaagt gatcgccaaa
     7321 cttaacgatc acaaaccggc ctctatcggc caggcttcgc gtatttctgg cgtcacgcct
     7381 gcggccatct ccattctgct ggtgtggctg aaaaaacagg gtatgctgcg tcgtagcgca
     7441 taacgcatta aaaatgcctg gtaagcaccc gcttaccagg caacgcatca agaacaggta
     7501 atcaccgtgc tcaacaaact ctccttactg ctgaaagacg caggtatttc gcttactgat
     7561 caccagaaaa accagcttat tgcctacgtg aatatgctgc ataaatggaa caaagcgtac
     7621 aacctgactt cggtccgcga tcctaatgag atgctggtac gccatattct cgatagcatt
     7681 gtggtggcac cgtatctgca aggtgaacgg tttatcgatg tcggcaccgg accaggactg
     7741 ccaggcattc cactctctat cgtgcgtcct gaagcccatt tcactctgtt ggatagcctt
     7801 ggtaaacgcg tgcgtttcct tcgtcaggtg caacatgagc ttaaactgga gaatattgaa
     7861 ccagtacaga gcagggtaga agagtttcct tcagagccgc catttgatgg cgtaattagc
     7921 cgcgcttttg cctctctgaa cgatatggtg agctggtgcc accatcttcc tggtgagcaa
     7981 ggccgtttct acgcgctgaa agggcaaatg ccggaagatg aaatcgcttt gttgcccgaa
     8041 gaatatcagg tcgaatcagt ggttaaactt caggttccag ccctggatgg cgaacgtcat
     8101 ctggtggtga ttaaagcaaa taaaatttaa tttttatcaa aaaaatc
//
LOCUS       DAZPCS010000158         8080 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZPCS010000158 DAZPCS010000000
VERSION     DAZPCS010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8080
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.159"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            1..1331
                     /gene="yjeM"
                     /locus_tag="ACHUQ7_003366"
                     /pseudo
     CDS             1..1331
                     /gene="yjeM"
                     /locus_tag="ACHUQ7_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(1482..4793)
                     /gene="mscM"
                     /locus_tag="ACHUQ7_003367"
     CDS             complement(1482..4793)
                     /gene="mscM"
                     /locus_tag="ACHUQ7_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFR1136639.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNIRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNRQLEDALKEVNEATHRYLFWASDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFVSGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(4815..5783)
                     /gene="asd"
                     /locus_tag="ACHUQ7_003368"
     CDS             complement(4815..5783)
                     /gene="asd"
                     /locus_tag="ACHUQ7_003368"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFR1136640.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(5880..6932)
                     /gene="rsgA"
                     /locus_tag="ACHUQ7_003369"
     CDS             complement(5880..6932)
                     /gene="rsgA"
                     /locus_tag="ACHUQ7_003369"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFR1136641.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            7027..7572
                     /gene="orn"
                     /locus_tag="ACHUQ7_003370"
     CDS             7027..7572
                     /gene="orn"
                     /locus_tag="ACHUQ7_003370"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFR1136642.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            7783..7858
                     /locus_tag="ACHUQ7_003371"
     tRNA            7783..7858
                     /locus_tag="ACHUQ7_003371"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7816..7818,aa:Gly,seq:gcc)
BASE COUNT         1844 a         2085 c         2194 g         1957 t
ORIGIN      
        1 ttaatgatgg ctgaaatggg agctgcttat cgcaaagaag aaggtggtat ctattcctgg
       61 atgaataata gtgtcggacc acgttttgcc ttcattggta cgtttatgtg gttttcctct
      121 tatatcatct ggatggtgag tacctccgcg aaagtttggg taccgttctc aacattcctc
      181 tatggtagtg acatgaccca gcactggcgt attgccggac tggagcctac gcaggtggtt
      241 ggtctgctgg cagtagcatg gatgattctg gtcacagtcg ttgcttcaaa ggggattaat
      301 aaaattgccc gcattactgc ggtgggcggt attgcagtaa tgtgtctgaa tttagtattg
      361 ctgttagtaa gcattactat tttgttatta aatggtgggc atttcgcgca ggatattaat
      421 ttccttgcat caccgaaccc gggttatcag tccggtctgg caatgctatc gtttgtggta
      481 tttgctattt ttgcctatgg cggaattgaa gcggttggtg gtctggtcga taaaacggaa
      541 aatccagaaa agaactttgc caaaggtatt gtttttgccg ctattgttat ttcaatcggt
      601 tattcgctgg caatattttt atggggcgtc agcacaaact ggcagcaggt attaagtaat
      661 ggttccgtta accttggcaa tattacctat gtgctgatga agagcctcgg ggtgatgctg
      721 ggtaacgcac tgcatttgtc acctgaagcg tcattgtcgc tgggcgtatg gtttgcgcgt
      781 attaccggac tttcgatgtt cctcgcttat accggtgcgt ttgctattca ccgctgaaag
      841 ccatcatcca ggggacgccg aaagcgttgt ggccggaacc gatgacgcgc ctgaatgcga
      901 tggggatgcc gtctatcgcc atgtggatgc agtgcgggtt ggttactgtc ttcattctgc
      961 tggtttcgtt tggtggcggt accgcatcgg cgttctttaa caagctgacg ctgatggcga
     1021 acgtgtctat gacgcttcct tacctgttcc tcgcgctggc tttcccgttt tttaaagcac
     1081 gtcaggatct cgacagacca tttgtgattt tcaaaacgcg tatgtcggca atgattgcga
     1141 cggtggttgt cgttctggtg gtgacatttg cgaacgtctt caccattatt cagcctgtgg
     1201 ttgaagccgg agactgggac agcacattgt ggatgattgg cggccctgtc ttcttctcgc
     1261 tgttagcgat ggcgatttac cagaactatt gcagtcgcat ggcgaataaa cctgagttag
     1321 ctctcgactg atcatatgaa atcctgcaac tcatcgtctc tccggcgatg agttgtcagg
     1381 aagtaatgcg tatggatgat actctccagg gccggtgata actgaagatg atgttaagct
     1441 gctaaatgag ccacgttgac atcatttggc agcttcatgc attacaaact tcccgcctga
     1501 cgacctttgc ccgcagacgt cagcgttctc cccgtttgtt taccgttgag gctttccaga
     1561 cgcatctgga agggcgggaa tggcatatcg ataccgtggg cgtggaagcc tgccagaatc
     1621 agctggtgga tctcatggcg tagcggcata cggtgaccca tctcagcggc gtaaatacgc
     1681 agctcgaaaa tctgaatccc ctgttgcaga tccaccagga agacttccgg tgccgggttg
     1741 tcgatcacca gcgagcagcg acgcgctgcg gtgagcagga tttccgtcac ttcttcactg
     1801 ttggcatcgg caggggccgg aatcgtcaac actacgcgcg tgaccgagtc agagagcgac
     1861 cagttgataa actgctcggt aataaacgcc ttgttcggca cgattatctc tttacggtcc
     1921 cagtcgctga tggtggtggc gcgggtgtta atttttgtca cgctaccagt aagatcgcgg
     1981 atcgttaccg tatcgccaat gcgaatcggt ttttcgaaca ggatgatcag gccagagaca
     2041 aagttggcga aaatttcctg caaaccaaaa ccgagaccaa caccgagcgc ggcaaccagc
     2101 cactgcaatt tcgaccactc aataccaatc attgagaagc cgaccagccc gccaatcagc
     2161 atcagcagat attttgtgat ggtggtgatg gcgtaacccg tacccggcgt taaatccagg
     2221 tgctgcaaaa tcgccagttc cagcagcgcg ggcaggttgc gcaccagctg cgtggtgatg
     2281 ataaatacca gaatggcaat cagcaccgca ccgagggtaa ttggctccag actttctacg
     2341 ccctgtaccg tggaggtgac atcccacagc gaaatatttt cgaggaagcc gaaagcggaa
     2401 tggatttctg accacagcac aatgaccgaa agcagggcga tcaacatcaa aattgagcgc
     2461 accagccgca aggattgcgc actgatggca tcgagatcga cttcgctttc atcgacttca
     2521 attgctcctt ccgggctact gtgatgatgc gcttcctctt cgccacgcgc acgttgcgct
     2581 aacatctctg cccggcgatg cttcgcccgg tcaaacgcca gcctgcgacg ctggatcagc
     2641 atccagcggc ggataacgtg ataaaccacc agtagcagga accagatggc aaccgaggtt
     2701 tcaagcctcg ctaacagtgc ctgcgccgtt gccagataac ccaccgccga cgccagaatg
     2761 gcaaccagtg gtgcgccaat catcatgttc cacagcatat ggttggtaat gttgtcgccg
     2821 ctgccttctt tgttgagata caacggaata ccggcttttt tcaggcttag ggtgaccacc
     2881 gccagcgcac cgcaaatgag gataaagcaa agccgtccca gcgaaccgga aaattcgcgg
     2941 tcgtcgaggt tatcgaacat catcagcgcc ataatcagcc cgatgctcat caggtagtag
     3001 cgcatcccac gggaaacacg ttcgcgcggc cagccaaaat gagcgataaa caagccgttc
     3061 gggcgggcaa aggtggcgca aatcatcact acccacagca gcggcacggt ggccgttaca
     3121 ccatcgccaa tcgcgaccgc cagcggataa ggccacgcct cgcgcaagcc gtaacccagc
     3181 gtcatccaca gcaccggtaa cggtgacgcg acgagaatcg accagaaaag agtgcgcaac
     3241 gtcagccaga agtgatcctg agtcactttg ccgactttcg ccgccgaacg ttcaagaaaa
     3301 cgggtgaaat agcggcgcga gtaaatactg caaccgacca gaatcaacgc gccaaacagc
     3361 ggcaaaattg tctctttgct ggtcagcatc atcacactgg ctttgcccaa ctgactgaag
     3421 gtgtccagcg aaatgagacg acgcagatcc tgggcgattt ccagcggcca ggcgatggtc
     3481 atcgggcgca cgtcagaggc ccagaacaga tagcggtgcg ttgcttcgtt cacctctttc
     3541 agcgcatcct ccagttgcct gttggagact ttcagcttgg tcagttccaa tagtagcgtg
     3601 tcgccaccct gcaacaatga gttcagcaac tcacgctgag tgcgcagttg tgcttccaga
     3661 atacggtttt gctcggcggt cagcggctga ccgtcggcct gatggatttg ccgtagcagc
     3721 ggctgtttat taagtaaatc ctcataacgt aaccgttgca cacgcaactg cgccatttcg
     3781 gtatcaagct gttgtggttt tggcatttct ggtagccgtg ccacctgcgc ccgcagcgct
     3841 tcgccgagca ggttggacga tcccagccat tgcgactgtt cacgcagcgt attcaacgcc
     3901 tgccggacct gtaacgtctg gctggcagcc tgacgctgtt gcgaggcaac gagctccatc
     3961 cgctgcgcct gttgattcag cgctgccgat agttcgcggt taattttgaa ttgcgcgacg
     4021 atatctttcg gcaaatcggc actgttttct gccagtaatt cggtactttc cagcgcccgc
     4081 tccgcctcaa gctgacgttg gctattcaac tgattacgta aggcctgcaa atacgcatcc
     4141 agttgctggc tctctttttc cgccagctct gagcgtaagc gcgctaattc ctggcggtta
     4201 ttggcagaca gctgcgccag ctccagttca tcaacgagcg ccttaagacg tgcagagtca
     4261 gactgcaacg cgaaattttg tgcctgattg agcggagtat tgccggtaag cgttcccagg
     4321 cggcgctcga tctcatttaa ctgacggcgg gcgtcggttt gctgttgcgg cagttgattc
     4381 agcgaatcgg caatctcgcg ggcgcgctcc tgctcttgct gggcctgacg gcttttatcc
     4441 agcaactggc tgctgacctg gagaatttcc tgattcagcg cgtcggtaga cattcccggc
     4501 gacacgctgc gcggctcgtc acgaatgttg tttaattgtg cgcgcagagt agcggagagt
     4561 ttcggataat tatcgataac ttgctgatat tgtttgatgc gctcaaggga accttttcgt
     4621 tcctcaagcg catttaaggc agactggagc gcctctacga cttccggctg tgcgggtttc
     4681 gccgcttttg cctgctccag ttcctgagtg atttgtttgc tatcgggggc cgtcgcggcg
     4741 tacgcccccc aactgaggca ccaggccatc agaaaagtga taatcaggcg cacgtcagcg
     4801 tttcctttga tggattagac ctggtctttt ttatcgtcaa ccaatgggct ggcgtcgtgt
     4861 tctgcttcga tctcttcagc aggaagtggg gcgggttcag cgtctggcgt aacaaaggtt
     4921 tcggtagata ctgccagcgg ctggccaatt ttcgtgacag acaggctttc cagttgctca
     4981 accagattca ctttacccgg tgcaaacagg ttgataacgg tggaaccgag tttaaagcga
     5041 cccatttcct ggcctttcag cagtgccaca gaaccgtcgt tttccccggc aggccaggtc
     5101 cagcgcttga tgataccttc gcgcggcggc gtaatggtgc ccgcccagac cgtctcaatg
     5161 ctgccaacaa tcgtcgctcc gaccagaatc tgcgccattg ggccaaattc ggtatcgaaa
     5221 aggcaaatca cgcgttcgtt acgggcaaac agatttggca cgttctgagc ggtgagatgg
     5281 ttaacggaaa agagatcgcc cggcacgtag atcatctcac gcagaatacc gttgcacggc
     5341 atgtgtacgc gatggtagtc acgcggggag aggtaagtgg tcacaaacgt accgttgcgg
     5401 aacaggtccg ccatcagata gttgcctgcc agcagggctt cgaggctgta gttgtggcct
     5461 ttggcttgca ggattttatc ttcttcgatt ttacccaact ggctgataac gccatcggca
     5521 ggcatgacca gtacattcgg atcggtatcg attgggcgta cttcgtcacg cagcggacgg
     5581 acaaagaatt cgttaaaggt gcggtagctg gcggtgtccg gcttttgcgc ctctttcatg
     5641 tcgaccttgt agtatttaac gaacagatcg ataaccagtt ttgtcagcca tcctgcccgc
     5701 ttgcttgcgc cccaacccgc caggcgagta agccatagtt tcggcagaat gtactgtagc
     5761 gaaagtttaa atgaatttaa caaggtagcc tccaggccat tgttttgtcg ttcctgatcc
     5821 ggccaacatg ccggatcctg aaaaaaaggg gacgattcta acgacggtta gcttaattgt
     5881 cagtcatccg tatcagaaaa gtttttacgc gtttttacct gcgccatgct ttccagaata
     5941 cggtgatagt tttcgaaacg ggtttccgcg attttccctt cctcaaccgc ttcccggata
     6001 gcgcagcccg gatcggtatc gtgtttgcaa tcgcgatatt tacacagacc taaatagtca
     6061 tggaattcga caaagccctg agtgatttgt tccggctcca ggtgccagag gccgaactca
     6121 cgcacaccag gggaatcaat cacatcaccg ccgtgcggga agtgatacag ccgagcggcg
     6181 gtcgtggtgt gctgaccgag ccccgagttg tcagagacat cgttggtcag gatctctttt
     6241 tgcagcccca gcaacgcgtt cagcaggctg gatttaccga cgccagactg cccggcaaaa
     6301 atgctgatgc gcccggtcaa cgcctcttcc agcggtttca gcccatcctg agtatggctg
     6361 gaaaccatca atacgcgata accgatgttg cggtagatat ccatctgctc gttgacgaac
     6421 gccatgcctt cgtcgtccag caggtctatc ttgttgagca cgataatcgg ctcaatctgc
     6481 aaggtttcgc aggccaccag gtaacggtcg ataatattga gcgacagctc cggcaaaatg
     6541 gcggagacaa tgacaatctg gtcgatgttg gcggctatgg gtttcacgcc gtcgtagaaa
     6601 tccggacgcg ttaacaccga ggtgcgctca tgcaccgctt ccacgatccc tttgacattt
     6661 acgccttccg ccgccggttt acccggacgc cagactacgc ggtcgccggt taccagcgaa
     6721 cggatggtac ggcgaatatt gcagcggtga acgtcgccat cggcggattc cacatcagcg
     6781 tgcataccaa agcggctgat gacgatacct tcatcaggct cgccaaacag attgtcgtcg
     6841 tagtcgggct tctccttaga cgttttaaga cgacgctggt gattggcgtt cacgcggcgc
     6901 tgctggcctt tggagagttt atttttactc aatcgtactg actcctggtc gcccctgatg
     6961 ggcaaaacat ctatgataca cgcaattgtg gatcaatata gtcactgtga atgggtggaa
     7021 aatagcatga gtgccaatga aaacaacctg atttggatcg atcttgagat gaccggtctg
     7081 gatcccgagc gcgatcgcat tattgagatt gccacgctgg tgaccgatgc caatctgaat
     7141 attctggcag aagggccgac cattgcagta catcagtccg atgaacagct ggcgctgatg
     7201 gatgactgga acgtgcgcac ccataccgcc agcgggctgg tagagcgcgt gaaagcgagc
     7261 acgatgggcg atcgggaagc tgaactggca acgctcgaat tcttaaaaca gtgggtgcct
     7321 gcgggaaaat cgccgatttg cggtaacagc atcggacagg accgtcgttt cctgtttaaa
     7381 tacatgccgg agctggaagt ctacttccac taccgttatc tcgatgtcag caccctgaaa
     7441 gagctggcgc gccgctggaa gccggaaatt ctggatggtt ttaccaagca ggggacgcat
     7501 caggcgatgg atgatatccg tgaatcggtg gcggagctgg cttactaccg cgagcatttt
     7561 atcaagctgt aaaatttttc ctggtcacgt aagcgccgga atccacttgc ggcggcgtga
     7621 ttttgacgct aaatcgaacg tcttgctgat aatttcagca cttgagataa aaacgcaaaa
     7681 aaaacttttt tggggggttg cagagggaaa gatttctcgt ataatgcgcc tcccgtaacg
     7741 acgcagaaat gcgaaaatta cgaaagcaaa attaagtagt acgcgggaat agctcagttg
     7801 gtagagcacg accttgccaa ggtcggggtc gcgagttcga gtctcgtttc ccgctccaaa
     7861 atttgaaaag tgctgcaaag cacagaccac ccaatcgcag tatttcgaat tgcgacaagg
     7921 cggcaactga acgaatcctt gggaacttac aaaagtatgt gactaaggtg agtgaaggca
     7981 gccaaggcag tagcggttcg aaagacgaag attaagcggg aatagctcag ttggtagagc
     8041 acgaccttgc caaggtcggg gtcgcgagtt cgagtctcgt
//
LOCUS       DAZPCS010000159         8055 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZPCS010000159 DAZPCS010000000
VERSION     DAZPCS010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8055
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.160"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..77
                     /locus_tag="ACHUQ7_003372"
     CDS             <1..77
                     /locus_tag="ACHUQ7_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1136643.1"
                     /translation="AFPRVVKERPWKYPTAPKKSQSVA"
     gene            complement(91..282)
                     /locus_tag="ACHUQ7_003373"
                     /pseudo
     CDS             complement(91..282)
                     /locus_tag="ACHUQ7_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            280..604
                     /locus_tag="ACHUQ7_003374"
                     /pseudo
     CDS             280..604
                     /locus_tag="ACHUQ7_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(634..3546)
                     /locus_tag="ACHUQ7_003375"
     CDS             complement(634..3546)
                     /locus_tag="ACHUQ7_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFR1136644.1"
                     /translation="MFSKAFLRKISMFFARRKPAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYDASGEGSEVQNID
                     FLSLTEFLETLRIPEGREVTFSAFSDWLPRNRAIAALGAAHTLYEEFRGVIGAVASGN
                     GPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNLLSHQYLSLATP
                     RYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFSWSSLKSLFFRQ
                     QQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFVRSCGQAEGTIR
                     LLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKFFTNALYVALTR
                     AKHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHLLEKQGKQEQAD
                     TIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSLIYSLFPLYNAL
                     YREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQLLLERGADPLLTGNDGLAAYHQVLSAAVSTPRYAQQKSAQLYTLLKPE
                     SLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAARLAECVVHLPE
                     ALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLRLNHGQYILNPGLKIRKGDVWRAV
                     YELQSPEDLGHDLQTYLQDMSPELVDMLGGKKGFYERSEKSVGYWVGGIRRAAQKA"
     gene            4029..4871
                     /locus_tag="ACHUQ7_003376"
     CDS             4029..4871
                     /locus_tag="ACHUQ7_003376"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFR1136645.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQIKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            4874..5962
                     /gene="capU"
                     /locus_tag="ACHUQ7_003377"
     CDS             4874..5962
                     /gene="capU"
                     /locus_tag="ACHUQ7_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HFR1136646.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTR
                     KHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNDPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            5967..6917
                     /gene="virK"
                     /locus_tag="ACHUQ7_003378"
     CDS             5967..6917
                     /gene="virK"
                     /locus_tag="ACHUQ7_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HFR1136647.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEFLFPQKSVSLVQFIGKDGEDFDIQCSPSGFDREGELMLSLFFNKIVIARLTF
                     SVILTQNGHTAFIGGLQGAPKNTGPDIIRCATRACYGLFPKRIIFEAFCALMKACNVS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYKLPTQVVRKPLSN
                     IASKKRSEYRKRYALLDYIHETAIRSLDAYPVNSEHQDLN"
     gene            6982..7926
                     /gene="lpxM"
                     /locus_tag="ACHUQ7_003379"
                     /gene_synonym="msbB"
     CDS             6982..7926
                     /gene="lpxM"
                     /locus_tag="ACHUQ7_003379"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFR1136648.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWFTWFVLGMIAGISMFPPSFRDP
                     VLAKIGRWVGRLSRKARRRATINLSLCFPEKSDTEREIIVDNMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMI
                     ARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
BASE COUNT         2167 a         1806 c         1919 g         2163 t
ORIGIN      
        1 gggccttccc gagagtggta aaggagaggc cctggaaata ccccacagcc ccgaaaaaga
       61 gccagtcagt tgcttaactg actggcatta aggccgccgg ggacgttttt ttaccaccgg
      121 gagtttgggc ataacggtgc tttcagtaag tacgacaggt actgtggagg cacttccgta
      181 aagaagggca tcaagctcct gctccgacat acctgcaggc aaaggccatg aaagccctga
      241 tttccggaag cgagcgaaca gtccacaaac tgttgaacgt ggtgtcagcg gcggacaggg
      301 ataagccctc gtcagcagtt ccgtcagcac tgcgacagcg ttctttaccc ggtcaaaaca
      361 gcgttgcggt gcaccacgcc tgacggatga actgcgtgct cagggttacc actttaacgt
      421 aaaaaccgtg gcggcaagcc tgcgccgtca ggggctgagg gcaaaggcct cccggaagtt
      481 cagcccggtc agttaccgcg cacatggcct gcccgtgtca gaaaatctgc tggagcagga
      541 tttttacgcc agtggtccga accagaagtg ggcgggagac atcacgtact tacgtacaga
      601 tgaagttcgg ttacatcctg tcagcacgga acctcacgct ttttgagcgg cacgacggat
      661 cccccccacc cagtatccga cagacttttc actgcgttcg taaaaaccct tcttccctcc
      721 cagcatatcc actaattccg gtgacatatc ctgcagataa gtctgcaaat catgccccag
      781 atcttccggt gactgcaact cataaaccgc gcgccacaca tcacccttcc ttattttaag
      841 ccccgggtta agaatatact gtccgtggtt cagcctcaga aaaagctttt ttccataagg
      901 atttttgctg ttaacttcat gttgagagag ctggctgctg atataactcc ttcgtttgcg
      961 gcgttccggc agcaacgctt caggaagatg aaccacacac tctgccagtc gtgccgcact
     1021 gaaggcttcc gaaaaaaaca gagcacttcc cagatgcgtg tggaacagcg cctgcatcag
     1081 gatcaccaga aacattgcca tctgccggtt gtccagtttg atgaggcgtc cttccacctg
     1141 cagtgacaga ctttcaggtt tcagcagggt atacaactgc gcacttttct gttgcgcata
     1201 acggggcgta ctgaccgccg ctgacagaac ttgatgataa gccgccagcc cgtcattacc
     1261 ggtcagcaag ggatctgcac cacgctccag cagtaactga accagtgcaa tattacctgc
     1321 ccgggctgct gacatcagcg gagtaagatt aaacggcgaa cggtgatcaa caccataacg
     1381 ttcaatatcc cgcaacacgg caaccgggtt attcattgag tatggccgga aatacttcag
     1441 ctccagtaac tgcagagtct tcgttctggg ctgccttgtg ggcttaaaat cttcccggta
     1501 cagtgcatta tacagcggga acagggaata aatcagactg tactccagcg cctgcagttg
     1561 tatggtttta tcggctgtac ctgcgaggat atgctgtttc cactgtcgtg catcctctgc
     1621 ggtgataatc tgccatggca tctcagatgt ctgcaggatc cggctacgga tggtgtccgc
     1681 ctgctcctgc ttcccctgtt tttcaagcag atgtgcggtt ttctgccact catcacggct
     1741 ggaaatctct tcctgcagat tcagaggctc ttcctgatgc gtgagcgcaa gcagactcca
     1801 cagaggatgc tccacctgct gttcaacaag atacacattg tgtttagcac gggtgagcgc
     1861 aacatacagc gcgttggtaa aaaatttata gatttccgct gaacgatcct gcctgtcccg
     1921 cggacgggaa taacgggcct cgccctcaag atcagccggt gtaagaccct cgcaaatatc
     1981 atcaaatgcc tgccgggcag cgcttactat attgtaaagg atcaccgttt cgtattccag
     2041 ccccttaacc tcctgaaccg aaaagaccag cggcgtactg aaccagcatc gcgcctgtgc
     2101 tttctgttcc ggatgcatca caatgaccgc tacacgattc gaaagtgagg ttttggcatt
     2161 cagttcctgc tttgtctctt ccctatcgtc cagcaaccgg atggtccctt ctgcctgacc
     2221 gcagcttctg acaaaatggt gactttcccg atcaatagca ctaaagcgga cctgtttgag
     2281 tctcagcaac cggttagcca gtgcagtgac gtggtgcccg ttacggtaat tcgcctgcag
     2341 gatattaacg gttgtgtcgt ttccctgttg ctgccggaaa aagagacttt tgagcgaact
     2401 ccatgagaaa aagctcggat gaactatctg attggcatca ccacagagta aaaactgacc
     2461 gggatgacgt agcgttttca gtaccagttg cagttgaaca ggcgtcatat cctgaacttc
     2521 atcaacaaaa atgacatcat aacggggagt tgccaacgaa agatactgat gactgagcag
     2581 attactgtca tactgatgcg actgtttcag ccatgcgata taacgttcaa acaggacata
     2641 gaccgtcggg cggtcctcca tgccatagag cgactgccgt atccccagtg acagatacgc
     2701 ctcccgggac agcggcccat tcccggaggc aaccgcaccg atcacgcccc tgaactcctc
     2761 atacagggta tgggcagccc ccagtgcggc gatagccctg ttgcggggca gccagtcgct
     2821 gaacgcactg aaagtcacct ccctgccttc cggaatgcgc agagtttcca gaaattccgt
     2881 cagcgaaaga aagtcaatat tctgtacctc actcccttcc cctgaagcgt catagagagt
     2941 acgcgctttt tccaccagaa atgaggaaag gctgagatac agtatatcac cagcagcctg
     3001 cttcattttt tccagtacca gcgccgtctt accacttccc gccgagccaa caatcaccag
     3061 cggtagcggg tgttcgtaca gtgcctgctg atcatcatca aaggacagca ttttatccag
     3121 gcggtggaaa cgcccatgag acggattgat atacgtcagc gcttcagtcg cgatatcaac
     3181 aggatccggc actggttgtt gttgtaagcg cccttcatca atcaccacgt ttctgcgaag
     3241 aaaacgtgaa gtgttatagg catgattgcg aatatattcc agaacaaggc agatagtttc
     3301 accgtggtag cgatacagcg aaaacagtaa tcggtcccgg atactgagcc gggcgcggta
     3361 aagattggtg tcaattttac gaacatcagc ctgaacaaag ttatcggtgg caatagcctg
     3421 tgcaaatttt ttataacccg ggatcgtgtc aggattcaga gtatgataaa gacagatttt
     3481 catggctgcc ggtttccgtc gtgcaaaaaa catgctgatt ttacgaagga atgccttact
     3541 gaacaacctt gcccggaata ccccccgaaa tcagatggaa caatcagaaa aatgctctgc
     3601 agttatggaa taaagaaggt ggggtggtac gccgggaggg cgatccgctg gaaatggcgc
     3661 agtagctgca ggcctgtcat gatgcaggaa tagaagttcg tgtacaggtt aatgaaagcg
     3721 tcaccccgta aataaactct gcgctgaaat tccttttttt cattggagta cctgtgttgt
     3781 tctgaggtga acatatcacc tctgttcagg tagccaaatt cagtgtgcca cctcaaggtt
     3841 cttagtgagt cgtttttaaa agatatgata atatatcatt gtttatgaat tgttgatgtt
     3901 taatttagta aattatatca aatatgccat gggtttgcta tgttattcat ccagtatctg
     3961 gaaataaaat gcaccagctt tattgggttt tccttttgtc tctgcttgct aggtgccaga
     4021 aactttaaat gttaaacgaa gggggcattt tgttcaaagc caatcatgtt ccggtattaa
     4081 tgtatcatca tgtcagtcat tgccccggac tggttacact ttctcccgtg acattccgga
     4141 agcaaataaa atggttggct gaaaacaact ggaaaacact gtcatcagat gaactggagt
     4201 ttttttatcg gggcggaaaa ctcccccgga aaagcgttat gttgacattt gatgatggat
     4261 accttgacaa ctggtttcag gtatatcctt tattaaaaga gtttaatctc aaagcacata
     4321 tttttttaat taccggtttt attgggaatg gtccggttcg tcactctcct ggaaaggaat
     4381 attctcatcg cgattgcgaa catcaaatag cgacagggaa tgctgataat gttatgcttc
     4441 gctggtctga agtaaacgaa atgttgcaat ctggtctggt cgaatttcat gttcatacgc
     4501 atactcatac acgctgggat aaaaagttca gttcacgaga agaacagtgt aaacatcttc
     4561 ggcaggactt gttgagcggg agggaatatt tgaaagaaat gacaggaaaa tgcagtaaac
     4621 atttgtgttg gccggaaggt tattataaca aggattatat tcaggtcgct gaagaacttg
     4681 gattctatta tttatataca acagaaagaa gaatgaatgc tcctgctaaa ggaactacac
     4741 ggataggaag aataagcacc aaagaacggg aaagttgcgc atggctgaaa agacgacttt
     4801 tttattatac aaccccgttt ttttcatcgt tgctggcctt tcataagggg ccaagattac
     4861 cggatgatta ataatgaata tactatttac ggaatcatca ccgaatatag gtggtcagga
     4921 attacaggct gttgctcaaa tgaaggccct gaagaaaatg gggcattcag ttctgcttgt
     4981 ctgcagggaa aacagcaaaa ttgcttttga agccagtaaa ttggggattg atatcacatt
     5041 cgcgttattt cgaaacagtc ttcacatccc tactgcatgg agattactcg gaatagttca
     5101 tggttttcag cccaatgcaa tcgtttgtca cagtgggcat gatagcaata ttgttggttt
     5161 agtacggtta ttcactagga aacatccatt cagaattatc aggcaaaaga catatttgac
     5221 acgaaaaaca aaagtttttt caataaatca tttttgcgat gaggtgattg ttcccggaac
     5281 aagtatgaag acacatctgg agcaggaagg atgtcgaacc cgggttactg ttgtgcctcc
     5341 aggctttgat ttccagaaat tatacgttga ttcccgaaac agtttgcctc caaatgttct
     5401 ttcttggctg gcgtcccgaa ggggatgccc cgttattgct caggtaggaa tgttgcgccc
     5461 ggaaaaaggg cacgaattta tgttgaattt actgttccat ttaaaaatga atggacgaca
     5521 gttctgttgg ttgattgtgg ggtctggttc gcctgaactg cgggagcatt tacagtatca
     5581 gattgacagt atgggcatgc atgatgatgt ttttattgct gacaatgttt ttcctgccgc
     5641 ccccgtatat cgggttgcca gtctggtggt tctgccttca gaaaacgaat cgtttggtat
     5701 ggtactggca gaagcatcgg cattttctgt gcctgtactg gccagtcaga ttggtggaat
     5761 ccctgatgtt attcagaata accagaccgg gacattgtta ccagcaggta ataagcacgc
     5821 atggatgtgc gccctgaatg atttttttaa tgaccctggg cgtttttatc agatggctcg
     5881 ccaggcaaaa caggatatag aagagcggtt tgatattaat aaaactgcgt taaaaatact
     5941 cacattagcg aagcacaagt aacaatatgt tttctgtaag taacttatca tttatcggtt
     6001 tccttaaaag gattgttttt tcctcagatt cactcccggg gaagtgggaa cacagaaaat
     6061 tccggttcat gtacattttg cgatgtgcta taaatccggt tgccagtatt cgatattatt
     6121 acgaactgcg ttccttgcag tgcattgagg atattctggc aatacaaccc acgttgccag
     6181 caagaattca tcgaccttat ttgcataaag gggggcgagc ctggtcccgg gggcaatata
     6241 ttcttgagca ttatcgcttt gtgcaaaatt tgccagaaaa atattctgaa tttttgtttc
     6301 cacaaaaatc ggtatcatta gtccaattca tcgggaaaga tggagaggat tttgatatcc
     6361 agtgttctcc cagtggtttt gaccgggaag gagagttgat gttgtcattg tttttcaata
     6421 aaatagtgat tgcccggtta acattttcag tcattctgac tcaaaatgga cacaccgctt
     6481 ttattggcgg attacaggga gcccccaaaa ataccggacc tgatattatt cggtgcgcaa
     6541 cccgggcctg ttatgggctt ttcccaaaac ggattatttt tgaagcattt tgtgcgttga
     6601 tgaaagcttg taatgtctct gagtgtctgg cagtcagtga gcacagtcat gttttccggc
     6661 aattgagata ctggtatcag aaacgcaaaa cctttgttgc tgtctacagt gatttctggg
     6721 agtctgtagc agggaaaacc tgtggtgact ggtacaaatt accaacacag gtggttcgaa
     6781 agccgctaag caatatagct agtaaaaaac gctctgagta tcgaaaacga tacgctttgc
     6841 tggattatat ccatgaaact gctatccgct ctttggatgc atatcctgta aactcagaac
     6901 atcaggactt aaattaaggt ttgatgtatt ctctgaataa aatattaatg atgattttgg
     6961 taggggcatt cgcactaaat aatgaaaaaa tacaaatccg agtttattcc tgaatttaag
     7021 aaaaattatc tttcccctgt ttactggttt acatggttcg ttttgggaat gattgcaggt
     7081 atttcaatgt ttcccccttc attcagagat cctgtcttgg ccaaaatagg gcgttgggtg
     7141 ggcagattga gcagaaaagc tcgtcgcagg gcgacgatta atttatcgct ttgtttcccg
     7201 gaaaagagtg atacagaacg ggaaataatt gtcgacaata tgtttgccac agcattgcaa
     7261 tccatagtga tgatggcaga actggcaatt cgtggtccgg aaaagttcca gaaacgagtg
     7321 ttctggaaag gcctcgaaat tcttgaggaa attcggcaca ataacagaaa tgtgattttt
     7381 ctggttcccc atggctggag cgtggatatt cctgcaatgt tgctggcagc tcagggggaa
     7441 aaaatggccg ccatgtttca tcagcaacga aatccagtga ttgattatgt ctggaattct
     7501 gtacggcgta aattcggggg gcgcttacat tcccgggagg atgggataaa accatttatt
     7561 cagtcagtac gccagggata ctgggggtat taccttccag atcaggatca tggtcctgaa
     7621 tacagtgaat ttgctgattt ttttgcgacc tataaagcga cattaccaat tattggacgt
     7681 ctgatgaaca tcagtcaggc tatgattata ccgcttttcc cggtttatga tgaaaaaaaa
     7741 catttcctga ctattgaagt tcggccacca atggatgcat gcattgccag cgcggacaat
     7801 aaaatgattg cccgacaaat gaacaaaaca gtggaaattt tggtggggtc acatccggaa
     7861 cagtatatct gggttttaaa attgttaaaa acgcgcaaat caaacgaagc ggacccgtac
     7921 ccttgaaatt ataaagtaca ggtatttcca ctagttgttt cttacaggtt accaatcgaa
     7981 acacatcccc cttccgtgtg ttgcccgttt ggcacggagg actgtgtaga tgcagttcgt
     8041 taaatttaat ttatg
//
LOCUS       DAZPCS010000160         7923 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZPCS010000160 DAZPCS010000000
VERSION     DAZPCS010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7923
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.162"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..956)
                     /locus_tag="ACHUQ7_003380"
     CDS             complement(<1..956)
                     /locus_tag="ACHUQ7_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFR1136649.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKIEVNLGAGEAGS"
     gene            1202..1828
                     /gene="gstB"
                     /locus_tag="ACHUQ7_003381"
     CDS             1202..1828
                     /gene="gstB"
                     /locus_tag="ACHUQ7_003381"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HFR1136650.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(1825..2939)
                     /gene="yliI"
                     /locus_tag="ACHUQ7_003382"
                     /pseudo
     CDS             complement(1825..2939)
                     /gene="yliI"
                     /locus_tag="ACHUQ7_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
     gene            complement(3050..3433)
                     /gene="bssR"
                     /locus_tag="ACHUQ7_003383"
     CDS             complement(3050..3433)
                     /gene="bssR"
                     /locus_tag="ACHUQ7_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFR1136651.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            3646..4971
                     /gene="rimO"
                     /locus_tag="ACHUQ7_003384"
     CDS             3646..4971
                     /gene="rimO"
                     /locus_tag="ACHUQ7_003384"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFR1136652.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ANMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLMSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(5018..6346)
                     /locus_tag="ACHUQ7_003385"
     CDS             complement(5018..6346)
                     /locus_tag="ACHUQ7_003385"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFR1136653.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLVMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVILGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(6354..7923)
                     /locus_tag="ACHUQ7_003386"
                     /pseudo
     CDS             complement(6354..7923)
                     /locus_tag="ACHUQ7_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic diguanylate phosphodiesterase"
BASE COUNT         2101 a         2008 c         1786 g         2028 t
ORIGIN      
        1 gagcccgctt cgcctgcccc gagattcact tcgatcttat caccaaacca gacgcgctga
       61 gtcacaaagg tggcatcagg tttaattggc gtcacagttt caaagaagcg gaaaccccag
      121 gtcaataatt tctcagactc attaaaacgg atacggtcag ttttcgcccc cagcactacg
      181 gagattaaac gcatatcacc ctgggtagcc gaagcaacca gattatatcc cgcacctgcc
      241 gtggttcctg tcttcatgcc atcaacattc agattgctgc tccacagcag acggttacgg
      301 ttaggctgac gaattttgtt gaaggtgaac tctttctctt tatgaatggc gtactcttcc
      361 ggcacatcgt ggatcaatgc tttacccagc aatgccatat cgcgcgcggt actgaactgc
      421 cccggcgcat ccaggccgtg caccgtctgg aaggtagtgt tggtcagccc cagttttttg
      481 gcataaccat tcatcagacc aataaaggac tcctggctcc cggcaacgta atctgccagc
      541 gcaatacagg cgtcattacc ggactggata atcacacctt tgttcaagtc tgccaccgaa
      601 acctgatcgc ccggtttgag gaacattacc gatgaaccac gcagtgccgg atttcccgtc
      661 gcccaggcat ctttaccgac cgtcaccata tcggtgagtt taatcttatc ggccttaagc
      721 gcctgcccaa ccacatagct ggtcatgatt ttagtcaggc tcgcaggatc cagtttctca
      781 tccgcattgc cttctgccag cactttaccg ctggcgtaat ccattaaaat ccatgcacgc
      841 gcatccacgc tcggcgcttc aacggtttgt tcggccgcga atgccgttgg ggcaaaaagg
      901 aataaaaatg cagaacccgc tgcaagacca cgaaggagag aggagtattg cgtcataaat
      961 gccacccgag tatccattca aaaaaatacg ttatatcgcc gtatgataag ttcggtgatt
     1021 aataacgcac acacaaactt aaagaaaccg taagttggta aagtttttaa agtttatgca
     1081 atatcctgcc gcttcgctgc ttctggtgtc atttttttgc cagctgttgc ttaaaagtta
     1141 actttacttc accatcggag tcctgttcag tctgtggctg cctgtcggaa aaggggtgag
     1201 tatgattacg ctgtggggtc ggaataattc aactaacgta aaaaaagtat tgctgacgct
     1261 cgaagaactg gaactacctt atgagcaaat tctcgcgggc cgtgagtttg ggataaatca
     1321 cgatgctgat tttctggcga tgaaccctaa cgggctggtg ccgttgttgc gtgacgacga
     1381 aagtgatctc attctttggg aatcaaacgc cattgtccgc tatctggcgg cacagtacgg
     1441 gcaaaaacgc ctgtggatcg actcaccggc acgtcgtgcg gaagcggaaa aatggatgga
     1501 ctgggcaaac cagacgctca gtaatgctca tcgcgggatc ctgatgggat tagtcagaac
     1561 accaccggaa gagcgcgatc aggccgccat tgatgccagt tgcaaagagt gcgacgccct
     1621 gtttgccctg ctcgatgcgg aactggcaaa agtaaaatgg ttctctggcg acgagtttgg
     1681 tgtgggcgat atcgctatcg caccgttcat ctacaatttg tttaacgttg gcctgacctg
     1741 gacaccgcgt ccaaatctgc aacgctggta tcagcaactc actgaacgcc ccgcagtgcg
     1801 caaagtggtg atgattcccg ttagctaatt gcgtgggcta actttaagta attccccact
     1861 ggactcgtcg gtgagaacgt ataaataacc gtcgggtcca gtgcgaacat cacgaattcg
     1921 ctgccctctg tccgttaaaa tacggccatc ttctgtcact ttgtcgccgt tgacgctcat
     1981 cacaatgaca tctttatctt tcagctccca ataaataatt tttgctgcca ctgggggaat
     2041 ttatcgctgt tatagaaggc catgccgctc acagcgggcg aatctttcca gtaaaaaaca
     2101 ggttgctcgg tcccggcgac gatctcccct ttcgcttccg gtatcttaaa gcctgaatag
     2161 ttgattcccc aggttgccag cggccagccg tagtttttgc ctttttgcgg gatattaatt
     2221 tcatcaccac cgcgcgggcc atgttcattc agccacagtg cattactcca cggattcatc
     2281 gccattcctt gcggattacg aatgccataa gaccagatct cggcgcgcgc accggattcc
     2341 tttataaaag gattatcatc cgggatttcg ccctggtcgg tcagacgcac cagtttgccc
     2401 tgtaatttat ccagatcctg cgccgtcggg cgctgattgt tttcgcccag agcaataaaa
     2461 agataacctt taccgtcgaa taccagccgc ccgccaaaat ggttgccggt agacagtttt
     2521 ggcatctggc ggaagacggt gcggaagtcg gtcacttttg agagatcatc acttaagcgg
     2581 ccataaccca cagcagttcc ggctttgcca tcatcgccaa cttcggaata acttaaccag
     2641 atgcggcgag actgagcaaa atcaggcgct aaaaccacgt ccagcaggcc gccctgcccg
     2701 tgcgcccaaa cgtccggaac tccggaaagc ggcgcagata atccttttcc tgcttgccag
     2761 tgacgcaact cgccgccgcg cagagtgatt aacataccgt gattatcggg taaaaaggcc
     2821 agtgcccagg gatggtcgag tttgtcttgc agtacttcga catttaccgt tgcaggagcc
     2881 gcccagagag cggaagaaag acaaataagg ggcacaagga aaaaggattg tcgatgcata
     2941 acgcctccgg attgactctg gcttaaagcg tagtcagtgg aggagataac aaattcattt
     3001 ttacaaaaac ttaaacatga agggggagac gctttctccc ccttagtttt caggccttct
     3061 caagcatggc gtgcttctgc aggctctgga tactcagcgt taagctcatc agacaatttt
     3121 caagcttatc ggcgttgacg gtaataacag tcgggcaatc atggtgccca ctcatcaaac
     3181 atactgcggc tgtcgctaat gcttcttcag catgatgaag agcactccac tcttcctgat
     3241 ccagatgaag attcaaccgc agcgatttat cgtgcagttc gcgattcagt ttaaaaaagt
     3301 tatctcgtag atgattgctt tcgctgacgg acatgtatcc ttttgccttc ctcagttgca
     3361 cgtatgcagc gaggtcaacg cgtgcgtcga tcaaccggtt cagcagatcg attcgctgtc
     3421 tgtcaacgaa catacttcga tcctcctctt tccagccttg ttgcaattaa atagtatggc
     3481 tattttttga gcaaaatgct gtccaggatc aattcttcac gccgaattaa caaaatttac
     3541 atttccttcg cctccgccag cgaatgagat tgcgatacaa attttatcca gaactctgta
     3601 gaatccctgc cctgaccttt cctgtaactg aatatttttt aagctatgag caaagtaact
     3661 ccccagccga aaatcggctt tgtttccctt ggctgtccga aaaaccttgt cgattcagag
     3721 cgtattctca ccgaactccg cactgaaggt tatgacgtgg taccgagcta tgacgatgcg
     3781 aacatggtga tcgtcaacac ctgcggcttt attgacagcg cggtacaaga atcactggaa
     3841 gccattggtg aagcgttgaa tgaaaacggc aaggtaattg tgaccggttg tctgggggca
     3901 aaagaagatc agatccgcga agtccacccg aaagtgctgg aaatcaccgg gcctcatagc
     3961 tatgagcagg ttctggagca cgttcatcac tacgtgccaa aaccgaaaca caacccattc
     4021 ctgagcctgg tgccagaaca aggtgtgaaa ctgacgccgc gtcattatgc ctatctgaaa
     4081 atttcggaag gctgtaatca ccgctgcacc ttctgcatta ttccgtctat gcgcggcgac
     4141 ctgatgagcc gtccgattgg cgaagtgtta agtgaagcga aacgtctggt agatgcgggc
     4201 gttaaagaga ttctggtgat ctcgcaggat acttccgcct atggcgttga tgttaaacat
     4261 cgtactggct tccacaacgg cgagccggta aaaaccagca tggtcagcct gtgcgaacag
     4321 ttatcgaaac tggggatctg gacacgtctg cactacgttt acccttatcc gcatgtggac
     4381 gacgtcatcc cactgatggc agaaggcaaa atcctgccgt atctggacat tccgttgcag
     4441 cacgccagcc cgcgcattct caaactgatg aagcgtccgg gttctgtaga tcgccaactg
     4501 gcgcgcatca aacagtggcg cgaaatctgc ccggaactga ccctacgctc aacctttatt
     4561 gtcggcttcc ctggcgagac ggaagaagat ttccagatgc tactcgactt cctgaaagaa
     4621 gcacgtctgg atcgcgttgg ctgctttaaa tacagcccgg ttgaaggtgc agacgccaat
     4681 gccctgcctg accaggttcc ggaagaagtg aaagaagaac gctggaaccg tttcatgcag
     4741 ctgcagcagc agatttccgc cgagcgcctg caagagaaag tgggccgtga aattctggtg
     4801 attatcgacg aagtggacga agaaggcgcg attggtcgca gcatggcaga tgcaccggaa
     4861 atcgacggcg cggtttatct caatggtgaa accaacgtta agccgggtga tatcctgcgt
     4921 gtgaaagtcg agcacgccga tgagtacgat ttgtggggta gccgggttta attaattcag
     4981 gccgggcaaa atcatttgtc cggcctgcga ataatgatta ctgccagcta atctgtgccc
     5041 tgccgttttt cttactctta tacagccgct catccgccag cattttcaag cgttcgacgg
     5101 tagtacattc atttgaatgc gctacaccga tactggtgct taaattaatg cttcccacct
     5161 ggaaatgaac gatttctttt agtatcgata gcaactcttc cagcgtgccg ccagtataaa
     5221 tcaccgcaaa ttcgtcacct ccgaagcggt agactttacc tttatctacc gggatctgtg
     5281 atttaaggat tttcaccact ttcatcaaca cttcatcgcc cagcaaatga ccgaaggtgt
     5341 cgttaatgtt tttgaattta tcgacgtcaa acagacacaa ataaccgcct ttttccggca
     5401 gcgcttttaa atcttcatca aaggattttc tgcccattgc tttggtcagg tcgtccagga
     5461 acgccgagtt atacatactg tagagttttt tgacatcggc agccgaaaag aaaagcccgc
     5521 catccagtac gcgttcaaaa tggttattaa tgatggcttc tttctccaca ataccacgat
     5581 taatcactct gcgtaccaga tagaggcaac tggcacccaa tatcactgcc accaatgcgg
     5641 caaagaaaat aatcaatgta caaacccgca aataaacatc ttttttatac gcaaatacgt
     5701 acaatccggt cggcaaatag gcgaagtgct ccatcgcttc cgaaacggtg aagatggcgt
     5761 ttttagcatt atattcatca ccggtcaccg gtttaccgtt cttaaataga gagaacatca
     5821 ggttctcgct gtccatcacc agcgattttg acagtaaaga aaaacgctcc agaccaagca
     5881 aaaatatcag actacgattg tgagttaccg gatagaggta atagacgaag gttttaccgt
     5941 caaaagagat aaaccctgta tttttgacat gtcgatactt cacaacctgc ataatatctg
     6001 cacaataatt tttctcgaac tcctttacct gaatacattc gtcactggaa atcagataag
     6061 gaccgacatc gtacaaataa gagggggaaa ccgagtcggc gtaagctgtg cgcaaaccaa
     6121 ccaacagctg ttcctccgtg ggatttagcg tgttgataag atcaattgcc acgctacgcc
     6181 gatattcagc aatatttgcc accgcaaaag cagtatcaat aacggactgt ttcaggctat
     6241 acacccgttc ctttaccatt tgagtgtaga tcccgcaggc aactgctgaa atcatgataa
     6301 aaatcagcag actgactgta agtacgaact tattgattct ggacatacgt aaactactct
     6361 tttactaatt ttccactttt atcccaagcg gagaatggca tgggtttctg gtacagatac
     6421 ccctgagcct gaacacatcc taatttaatt aattcgcgcg caacgtcttc gctctctacg
     6481 ccttccgcaa tcaccgggat ccggttatag cgtgccagat cgaaaataat gcgcacaatt
     6541 tttcccgcag taccgaaaat atcgccggta aattgcgagt ccacctttat gctgtctgga
     6601 ttaatttcat aaaaacgttt caggtttgac aaaccggtgc caaagtcatc aatcgatatc
     6661 cgtacccctt gtttacgcag ttgttcaata ttgagataaa aatgttcatt aattcgctcg
     6721 atcacggttt cagtgatctc aacacagagg ctgttcgcct tcaggccatt ttctttcatg
     6781 atggtggtaa aacgctcatg aaagtgcggt tcgtttaact gccagggcga aacattaata
     6841 tgcagtaaga aatcatcctt cagcgacgca cgttgactaa aatgtctgaa ttaacccatt
     6901 gccgttttaa taatctgata acccagctca ttgataaacc cgatatcttc agcaatggta
     6961 atgaacttca gcggtgaaat gatcccccgc tccgcagaaa cccaacggca caacgcctcc
     7021 gcgctcagaa tggcgcgggt gttaatatca acgatcggtt gatagacgag gaatagttcg
     7081 ccaatttcca ttgcatggcg aatattacgc gcaatccaga tattctcttc tatttcgttg
     7141 agtttcgcgt catcacagaa aataaactcg gagaacatat ggtcacggat actggaaatc
     7201 gccagtttca gtttttctgc gtaactccag gcgttttccc cctgcaaagg ttcacaaatg
     7261 gcagacttat ggatgcgcag attgcggtag ccaaagctgc tgtcaaaatc gtttaccatc
     7321 gctattttac gggtaaatgt ctgtacattt tcccgacaca gaagtaaata gagatcggca
     7381 ttgaatgtca cgatatcgca gcattgcgca tatttctcgg caattcgctg acacaatttc
     7441 ctgacgattt tattgcgttc atcaacacca aacaccgcct ccacggcttc cagaccacac
     7501 agtttgatga tccccacagc cagatactga ttattgttgg actcaataat cgccatattt
     7561 ttaataaaac cgctttcctg atcgtaagag atcagtgaga gcaatttatt tgaccgttca
     7621 atttcggcat ctttcatttc cgcgatcgaa tttttgatgc tgataatctc tttcgcatcg
     7681 ccttcgcgaa ttttcagagc ctcgatctga tccgttgagt cgggcaaaaa cgccagcgta
     7741 ttaaccagct cggtcatttc agaaaattgc cgacggaaca cacgagtggt catgaaatat
     7801 accaccagca acacgataat aatgaacgca aacggtagat aaaaaagttt atctgatgcg
     7861 ttcaataaat taccgccagg ataataaaaa tagatttcga acaatgcatt atttggcact
     7921 gct
//
LOCUS       DAZPCS010000161         7913 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZPCS010000161 DAZPCS010000000
VERSION     DAZPCS010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7913
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.163"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(172..1197)
                     /gene="tdh"
                     /locus_tag="ACHUQ7_003387"
     CDS             complement(172..1197)
                     /gene="tdh"
                     /locus_tag="ACHUQ7_003387"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFR1136654.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(1207..2403)
                     /gene="kbl"
                     /locus_tag="ACHUQ7_003388"
     CDS             complement(1207..2403)
                     /gene="kbl"
                     /locus_tag="ACHUQ7_003388"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFR1136655.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(2678..3535)
                     /gene="yibB"
                     /locus_tag="ACHUQ7_003389"
     CDS             complement(2678..3535)
                     /gene="yibB"
                     /locus_tag="ACHUQ7_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HFR1136656.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRIEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            3839..4771
                     /gene="rfaD"
                     /locus_tag="ACHUQ7_003390"
     CDS             3839..4771
                     /gene="rfaD"
                     /locus_tag="ACHUQ7_003390"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFR1136657.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            4781..5827
                     /gene="rfaF"
                     /locus_tag="ACHUQ7_003391"
     CDS             4781..5827
                     /gene="rfaF"
                     /locus_tag="ACHUQ7_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFR1136658.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            5831..6823
                     /gene="rfaC"
                     /locus_tag="ACHUQ7_003392"
     CDS             5831..6823
                     /gene="rfaC"
                     /locus_tag="ACHUQ7_003392"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFR1136659.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            6820..>7913
                     /gene="rfaL"
                     /locus_tag="ACHUQ7_003393"
     CDS             6820..>7913
                     /gene="rfaL"
                     /locus_tag="ACHUQ7_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HFR1136660.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEVFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAY"
BASE COUNT         2138 a         1895 c         1820 g         2060 t
ORIGIN      
        1 tgtgagatat tatttttaca ggtgagagag tcacttcagg gtagagagat aatgagaaca
       61 gctatataaa agatcagcgc aattacgaac tctttattac actgacagga ttttacataa
      121 ttaacagatt atcctcagat aaaagggctg gaataccagc ccttgttcgt gttaatccca
      181 gctcagaata actttcccgg actggcccga acgcatagcg tcaaagccct tctggaaatc
      241 atcgatagag aaacgatggg taatgatcgg cgagagatcg aggccagact gaatcagcgc
      301 cgccatcttg taccaggttt caaacatctc acgaccgtaa atgcctttaa tgaacaagcc
      361 tttaaagatc actttggtcc agtcgataga catatcagac ggtggaatac ccagcatcgc
      421 aatacgaccg ccgtggttca tggtatcgag catggtacga aacgctggcg gcgcgccgga
      481 catttccaga ccgacatcaa agccttcggt catgcccagt tcagccatca catcattaag
      541 gttttctttc gcgacgttaa ccgcacgggt gatacccatt ttgcgcgcca gctcaaggcg
      601 gtattcgtta acatcggtga tcaccacatt gcgtgcacca acgtgtttcg ccaccgccgc
      661 agccataata cctatcggac ctgcaccaga aaccagcaca tcttcgccca ccagatcgaa
      721 cgacagcgcg gtatgcacgg cgttaccgaa gggatcaaaa atggaagcca ggtcgtcgga
      781 gatattgtcg gggattttga aagcgttgaa cgccggaatc accagatatt cggcgaagca
      841 gcccggacgg ttaacaccga caccaatggt gttgcggcac agatgggtac gaccgccacg
      901 acagttgcga cagtgaccgc aggtgatatg gccttcgcca gaaacgcgat cgccgatttt
      961 gaagcctttc acttcctgac caatacctac cacttcaccg acatattcat ggcccacgac
     1021 catcggcacc gggatggttt tttgcgacca ctcatcccag ttatagatgt gaacgtcagt
     1081 cccgcagatg gctgttttac ggattttaat cagcagatcg ttatgcccga gttccggtac
     1141 agggacgtcg gtcatccaga tgccctcttc cgctttcagt ttggataacg ctttcatctc
     1201 acatcctcag gcgataacgc ccagttgttt accaatacgc gtgaatgctt ctactgcacg
     1261 cgtaatttgc tcaggggtat gcgccgcaga catctgggta cgaatacgcg cctgaccttt
     1321 cggaacgacc ggatagaaga aaccggtgac gtaaatgccc tctttttgca gctctcgggc
     1381 aaatttctgc gccactaccg catcaccaag catgaccgga ataatggcgt gatcggctcc
     1441 cgccagggta aagcccgccg ccgacatttg ctcacggaac tgacgcgcgt tcgcccacag
     1501 acggtcacgc aactcgctac ccgcttcaac catctccagc actttgatgg aagcggcgac
     1561 aattgctggt gccagcgagt tggagaacag gtacggacga gaacgctggc gcagccactc
     1621 aaccacttct ttgcgcgccg cggtataacc accagaagcc ccgcccagcg ctttaccgag
     1681 cgtgccggtg atgatgtcga cacgccccat cacatcgcag tattcatggg aaccacgacc
     1741 attttcaccg acaaaaccga ccgcgtggga gtcgtctacc atcaccaggg catcgtattt
     1801 atctgccaga tcgcaaacgc ccttcagatt ggcaatcacg ccgtccatcg agaacacacc
     1861 atcggtggca atcagcacat gacgcgcacc ggcttcacgc gcttctttca gacgagcttc
     1921 aagctcctgc atatcgttgt tggcatagcg atagcgttta gctttacaca gacgcacacc
     1981 atcaataata gacgcgtggt tcagtgcgtc ggagataatg gcgtcttccg cgcccagcag
     2041 cgtttcaaac aggccaccgt tagcatcaaa gcaggaagag tagagaatcg catcttccat
     2101 ccccaggaat gccgccagtt tttgttcaag ctctttatgg ctgtcctgag tgccgcaaat
     2161 aaaacgcacc gaagccatgc cgaaaccgtg agaatccatt cccgcctttg ctgccgcaat
     2221 cagatccgga tgattcgcca gcccgagata gttgttggca caaaagttaa tgacgtggct
     2281 tccatcagcc acggtgatat ctgcttgctg cgcagacgta ataatgcgct cttctttaaa
     2341 caacccttcc gcccgtgcgg tttccagatc gttggttaac tgctgataaa attctccacg
     2401 cattgcgatt ctccagacag ggcaaattcc agcacatatt acccaaactt ataggtcgcg
     2461 acgagatgat gcgttaacac ttctgcaaaa ttcaggataa ataacgtgta tcttgatgtc
     2521 tctcaacacc tacagatagt agaaaaactg tacagttttg gctgagcata atgatgagtg
     2581 attttactgc attgatataa aaagaaaaaa tgatgccaaa gcatcatttt tcctatctca
     2641 gttggcatca aaacgctacc cactaatgga gggaaaccta ttttcttgaa aggaaaattc
     2701 tcagcgcctg catttttgcc cctaaagtgt tactcctgaa gcagcgaaac aaatcgaacc
     2761 attttcctct ccccagatag tttaggttga agagatcgga acgtttgtaa tagcacatga
     2821 caaaaatgcc ctggtcatcg tccacaatgt tattgtttag tgttattttt tgactttcca
     2881 gaactaattt atagaattct ttccatttat gctgtgaacc aacaattgcg cccccaataa
     2941 tatatacatg attgccaatc atgaaatcaa aagcctgttg ttgagatgta acagtaagcc
     3001 ctttttttat agtgaaaaga tgcatcttat tttcatcaaa tgggaaatcc caaattttca
     3061 atcctcgagt tacatttggc ttacggcaat agccaaaatc tatccacgca acaagaggcg
     3121 ttttcactaa acccatgttg atagctttat ttacaaaata ggccttaagg ttacatacca
     3181 aaacatactc tggtgaccag tactctgggt tttttaattg tcgaggctca agtctatttg
     3241 taaatgactc atctttttga attttttcga tacgacttct gatatatcta aatttctttt
     3301 ttatatcgat aacgataacc gttgttggtt ttccgttgcg aatcgcttca atccttggtt
     3361 tcaaatcggg agaagtgaaa ataatcatct cattttcaag tgcggccaga cgctcaaagt
     3421 aactaaaata aacatcaact gaacgagcta gtttttcacg gaacccttta ttagcagtcc
     3481 aatcccctct accaatgtca aaatatgcag tgataattgt ggtggatgat ttcattacta
     3541 ataagaccat taatataaaa aaagaattat atcataaata ttaacagaat gtatactgta
     3601 tattatccat tcaaatgatt ccgttattta tcatacatcg gaatattgat actaaagcac
     3661 tattcacatg caaaaccaac atccgccatg aaggactagc taaaacccaa actagtttgt
     3721 tgcaattagc atccttgcac ctctatgtaa agggctgaag ggattcggat gtgatggtat
     3781 gattacagac attcgtgtct gagattgtct ctgactccat aattcgaagg ttacagttat
     3841 gatcatcgtt accggcggcg cgggctttat cggcagcaac atcgttaagg ccctgaatga
     3901 taaaggcatc accgatattc tggtggtgga caacctgaaa gacggcacca agtttgtgaa
     3961 cctggtggat ctggatatcg cggactatat ggataaggaa gacttcctga tccagattat
     4021 ggctggcgaa gagttcggtg atgtcgaagc gattttccac gaaggcgcat gctcttccac
     4081 caccgagtgg gatggcaagt atatgatgga taacaactat caatactcca aagagctgct
     4141 gcactactgt ctggagcgcg aaatcccgtt cctgtatgcc tcttccgcag ccacctacgg
     4201 cggacgcacc tccgacttta ttgaatcccg cgagtacgaa aaaccgttga atgtctacgg
     4261 ttactcaaaa ttcctgtttg atgaatatgt tcgtcaaatc ctgccagaag cgaactcgca
     4321 gattgttggc ttccgctatt tcaacgttta tggaccgcgt gaaggccata aaggcagcat
     4381 ggcgagcgtc gctttccatc tcaacaccca gcttaataac ggtgaatcac cgaagctgtt
     4441 cgaaggtagc gagaacttca aacgtgactt cgtctatgtg ggcgacgtgg cagatgtaaa
     4501 cctgtggttc ctggaaaatg gcgtttccgg tatcttcaac ctcggcaccg gtcgtgcaga
     4561 atccttccag gcagtagcag atgctacgct ggcttatcac aagaaaggcc agatcgaata
     4621 cattccgttc ccggataagc tgaaaggccg ctaccaggcg ttcactcagg cagatctgac
     4681 aaatctgcgc gcggcgggtt acgacaaacc gttcaaaacc gttgctgaag gtgtaacgga
     4741 atacatggcc tggctgaatc gcgacgcata agagctctgc atgaaaatac tggtgatcgg
     4801 cccgtcttgg gttggcgaca tgatgatgtc gcaaagtctc tatcgcacgc tccaggcgcg
     4861 ctatccccag gcgataatcg atgtgatggc accggcatgg tgccgtccat tattatcgcg
     4921 gatgccggaa gttaacgaag ctatccctat gcctctcggt cacggagcgc tggaaatcgg
     4981 cgaacgccgc aaactgggtc atagcctgcg tgaaaagcgc tacgaccgcg cctacgtctt
     5041 accaaactcc ttcaaatctg cattagtgcc tttcttcgcg ggtattcctc atcgcaccgg
     5101 ctggcgcggc gagatgcgct acggtttact caacgatgta cgcgtgctcg ataaagaagc
     5161 ctggccgcta atggtggaac gctatgtcgc gctggcctat gacaaaggca ttatgcgtac
     5221 cgcacaagat ctgccgcagc cattgttatg gccgcagttg caggtgagcg aaggtgaaaa
     5281 atcatatacc tgtaatcaat tttcgctttc atcagaacgt ccgatgattg gcttttgccc
     5341 gggtgcggag tttggtccgg caaaacgctg gccacactac cactatgcgg aactggcaaa
     5401 gcagctgatt gatgaaggtt atcaggtggt tctgtttggc tctgcgaaag atcatgaagc
     5461 gggcaatgag attcttgccg ctttgaatac cgagcagcag gcatggtgtc ggaacctggc
     5521 gggggaaaca cagcttgatc aagcggttat cttgattgca gcctgtaaag ccattgtcac
     5581 taacgattct ggcctgatgc acgttgcggc ggcgctcaat cgtccgctgg ttgccctgta
     5641 tggtccaagt agcccggact tcacaccgcc gctatcccat aaagcgcgcg tgatccgtct
     5701 gattaccggc tatcacaaag tgcgtaaagg tgacgctgcg gagggttatc accagagctt
     5761 gatcgacatt actccccagc gcgtactgga agaactcaac gcgctattgt tacaagagga
     5821 agcctgacgg atgcgggttt tgatcgttaa aacatcgtcg atgggcgatg ttctccatac
     5881 gttgcccgca ctcactgatg cccagcaggc aatcccaggg attaagtttg actgggtggt
     5941 ggaagaaggg ttcgcacaga ttccttcctg gcacgctgcc gttgagcgag ttattcctgt
     6001 ggcaatacgt cgctggcgta aagcctggtt ctcggccccc ataaaagctg aacgcaaagc
     6061 gtttcgtgaa gcgctacaag cagagaacta tgacgcagtt atcgacgctc aggggctggt
     6121 aaaaagcgcg gcactggtga cacgtctggc gcatggcgta aagcatggat tggactggca
     6181 aaccgctcgc gaacctttag ccagcctgtt ttacaattgt aagcatcata ttgcaaaaca
     6241 gcagcacgcc gtagaacgca cccgcgaact gtttgccaaa agtttgggct atagcaaacc
     6301 gcaaacccag ggcgattatg ctatcgcaca gcattttctg acgaacctgc ctacagatgc
     6361 tggcgaatat gccgtatttc ttcatgcgac gacccgtgat gataaacact ggccggaaga
     6421 acactggcga gaattgattg gtttactggc tgattcagga atacggatta aacttccgtg
     6481 gggcgcgccg catgaggaag aacgggcgaa acgactggcg gaaggatttg cttatgttga
     6541 agtattgccg aagatgagtc tggaaggcgt tgcccgcgtg ctggccgggg ctaaatttgt
     6601 agtgtcggtg gatacggggt taagccattt aacggcggca ctggatagac ccaatatcac
     6661 ggtttatgga ccaaccgatc cgggattaat tggtgggtat gggaagaatc aagtagagtg
     6721 tcgttctaca agtatgtctc ttgcagattt gccagctcaa accgtttttc aaaatttaaa
     6781 tcttgaaata ataaccaata agttgacatc ggagataaga tgacctcaac attatttttc
     6841 tctctcgaga aaaaaaactg gatagcgtac tggaacagag ctctcgtatt cttattcatt
     6901 accacctatt ttttgggtgg gattacaagg tataaacatc ttattgttat tcttatgaca
     6961 ataacgacaa tcgtctatct ctgcaaacgg ccaaaacact atctctcact atttaaaaca
     7021 tttctttttg gtagtgttgc catattaact attgctgcat tgctgtcact tcttcaatcc
     7081 cctgatgcag gtgctagcat gaaggaagtt ttcaaagcta ttattgagaa tactttacta
     7141 tgcacaatag caataccggt catattgaga gacgagaaaa gagaagatgt cgaaaaaatc
     7201 gttttcttct catttattag tgcgttgggc ttacgctgtt tttctgaatt gattacctat
     7261 tataaggact atcaacaagg gataatgcca ttcgcagatt atagacaccg tagcatttct
     7321 gactcgatgg tctttttatt ccctgcattg ttaaatctct ggcttatcaa atcagcaaaa
     7381 taccgcattt cttttgtggt tctaagcgtt atttttattt ttctgatatt aggaacttta
     7441 tccagagggg cctggctttc cgtgttagtc attggattaa tatggattct gatgtttaaa
     7501 caatggaagt tactattagt aggagtaatg gtttccatca ttgcattgtc ggttattttc
     7561 acacataagg agatgaccgc aaagctaacg tataaacttc aacaaactaa tagttcttat
     7621 cgctatgcaa atggtactca aggcagcgca ctcgatctaa tattagaaaa tcctgttatt
     7681 ggttatggtt acggtaacgt tgcatataaa gatgtctata ataaacgtgt cattgattat
     7741 ccagaatgga cctttagaca atcaataggg ccacataatt ttgcgctatt catctggttt
     7801 ggcactggtt tattagggct ggtaagtctt atgatgctat actgtgcaat attgaaagag
     7861 tgtataaaaa atggcgtcaa gaataaatat cgctcaccat ataatgcata tta
//
LOCUS       DAZPCS010000162         7849 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZPCS010000162 DAZPCS010000000
VERSION     DAZPCS010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7849
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.164"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..668
                     /locus_tag="ACHUQ7_003394"
     CDS             <1..668
                     /locus_tag="ACHUQ7_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR1136661.1"
                     /translation="AQIKMADGTLNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAY
                     MAEVNLYKVIAPYVNLFKADLSFSKLDLINFEHADLSRVNLNKAILQNINLIDSKLFC
                     TWLTNTFLEMVICTGSNMANVNFNNANLSNCHFNCSILTKACMFNTRLYRVNFDEASV
                     QGMGISILRGEENIPIDSDTLVTLQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQ
                     HAD"
     gene            complement(822..2471)
                     /locus_tag="ACHUQ7_003395"
     CDS             complement(822..2471)
                     /locus_tag="ACHUQ7_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFR1136662.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(2622..3971)
                     /gene="ghxP"
                     /locus_tag="ACHUQ7_003396"
                     /gene_synonym="yjcD"
     CDS             complement(2622..3971)
                     /gene="ghxP"
                     /locus_tag="ACHUQ7_003396"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFR1136663.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLAGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(4677..5141)
                     /gene="soxR"
                     /locus_tag="ACHUQ7_003397"
     CDS             complement(4677..5141)
                     /gene="soxR"
                     /locus_tag="ACHUQ7_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFR1136664.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            5227..5550
                     /gene="soxS"
                     /locus_tag="ACHUQ7_003398"
     CDS             5227..5550
                     /gene="soxS"
                     /locus_tag="ACHUQ7_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFR1136665.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(5553..7130)
                     /gene="pdeC"
                     /locus_tag="ACHUQ7_003399"
                     /pseudo
     CDS             complement(5553..7130)
                     /gene="pdeC"
                     /locus_tag="ACHUQ7_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
     gene            7559..7840
                     /locus_tag="ACHUQ7_003400"
     CDS             7559..7840
                     /locus_tag="ACHUQ7_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HFR1136666.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT         2160 a         2044 c         1810 g         1835 t
ORIGIN      
        1 tcgctcagat aaaaatggcc gacggaactc taaatgcatg ctccgctatg catgttcaat
       61 tctacaatgc agcaatgaat agagccaata ttaaaaatac cttccttgac tattcaaatt
      121 tttatatggc gtacatggct gaggtaaatc tttataaagt aatagcgcca tatgttaatt
      181 tatttaaagc cgaccttagt ttctctaaac tcgatttaat taactttgaa catgctgatc
      241 tgtctcgcgt caatctgaac aaagcaatcc tccagaatat aaacttaatt gatagcaaac
      301 tcttttgtac gtggctgaca aatacattcc tcgaaatggt tatatgtacc ggctctaata
      361 tggctaatgt taattttaat aatgccaatt taagcaactg ccatttcaac tgttctattt
      421 taacaaaagc ctgtatgttt aatacccgtc tctatcgggt taatttcgat gaggctagcg
      481 tccagggaat gggcatttcc attctccgtg gggaggaaaa tatccccatt gatagtgata
      541 ccctggtaac actacagaaa ttctttgaag aagattgtac ctctcatact ggcatgtcac
      601 aaactgagga taatattaat gcagtcgcta tgaagattac tgcagatatt atgcaacacg
      661 cagattgagg ttaagtcgaa gaacagccaa tgaattgact ttacaagaga gataacttat
      721 ttgttttaga atcatgaatg ttcgcccgag ctaacctgtt aaaggtgcaa aagattgcac
      781 ctttttcaga ttacctcggg cctggttacg tcgctttcgg cttactgatt ttcctcaatt
      841 agcaacgctt caagcaaatc gagatcgtgc agtaattttt gcagcgtttc gttgctgatc
      901 tcccgcgtgg cgcgcaggtg gtacagttca gcacgttcag aacgcaatgc cgccagacgg
      961 aagcgacgct caaggttctc ttcctgcacg gaactttcaa cgtcgttacg tccatcggcg
     1021 cgacgacgca ggttaccaat gacgcgagaa ctgacctccg taagcaactg gttatcgata
     1081 ttctcttcgg tatccgccgc cagacgctcc tccattttct ggatcgccac aatcgccact
     1141 tctgccgttg ccgctcgcgc aatacgttct tctttcaatt gttgcgaatg gtccgcgact
     1201 tcaatgtgtt gtagcagaat aggcaacatc accacgccga caaacagcga gaagagaatg
     1261 acgccagccg ccaggaacac cagctcatag cgcgccggga agacgttacc atccggcaag
     1321 agcagcggga ttgagagcac accggccaga gtgatcgccc cacgcacccc agcgaaagac
     1381 gcgatcagga tttctcgtgt ggtccacgaa ccaaactcca tcggcttctt cttcaggaag
     1441 cggttgctga actttttcat cgtccacagc cagccgaaac ggaccagcat cagcgccgca
     1501 tatatcagaa taatatcggt aaacagcatc cagatttcga cgttagggtc gatttctgcc
     1561 gccatcagcg acgtctccag aatacccggc agttgcagac ctaacagcag gaacaccatg
     1621 ccgttaaaga caaattccag catcgcccag gtgctgtttg cgcgcaggcg cattgccagt
     1681 ggcgcacggc gcatcacacc ggagcgggtg atggtcatcc cggcggcaac cgcagcgagg
     1741 atgcccgaaa cgccaatatg ttcggcaatc agataagaag cgaatggcag caagaacagc
     1801 agaacgatct gcgtcgcggg ttcatcaccg ccccagcggc tgaggaatcg cagcgagcga
     1861 ccatacagcc agctcaccac aaaaccggca agaataccgc caatggctac tttcataaat
     1921 tcgaccgtcg caccgccgac agtgaagatc atcgtcccca tcgccactgc cacggcaaac
     1981 ttcaacgaca ccaggccgga agcgtcattc atcaacgctt cgccctgcaa aatgcccatg
     2041 atttttttcg ggatgcgccc ttcgccaaca atcccggaga gcgccacagc atcggtcgga
     2101 gaaagtaccg ccgccagcgc aaaggcgggg atcagcggaa tgcccggcac cacccagtaa
     2161 ataaggaagc caatgccgac cacggtgacc accaccagcg ccagcgcgag gccgaaaatc
     2221 tctcgaccat gttcgagaaa ttcacgggtc ggcgttttcc agccatcagc gaacagcaac
     2281 ggcgggataa acaagactaa aaagagttca ggatcaaact ccacatgcaa accaaacgtc
     2341 ggccacgcca gtagcgcacc gatggcgatt tgcataagcg gaagcgggat ctgaaagggc
     2401 atgacacgag tgaccacccc ggacagcgag accacgaggg tcattatcag tatggtgaag
     2461 aagatttcca tgcgttccct gtttgcgatg ttttttatga tctgagaaag acgttatgcc
     2521 tgaattctca ttttttcaga atatcgcaaa agctcacaca atgtgcggag aaaataaaaa
     2581 cgggcagcca ttgccacccg ttttcagaat ccgatgaaag attagatagc ccagccaccc
     2641 gcatagaagg tcaccagcgc cacggcgata acgaccgtac cgatgttcaa cttgcgccat
     2701 tcaccggaaa ccagacgacc aatcaccaga gtcgcgaagc cgatcatgat gcctgttacg
     2761 atgttacagg tcagcacgat gaataccgcc gtaaccagac ccgccatcgc atcaacaaaa
     2821 tcagcaaagt cgattttcgc cacgttgctc agcatcagca ggccaacgta catcagcgcc
     2881 ggagccgttg cataccccgg aacgaggtaa gaaagcggag agaggaacag aatcagcagg
     2941 aacagcacgc caacggtgat agccgtcaac ccggttttac cgcccgccgc cgtacccgcc
     3001 gcagactcga tgtataccgc tgccggagct gcacccacca ggccagagaa aacgctgctc
     3061 atggagtcag tggtcagtgc tttcccgccg tcgatgatct gcccatcttt atccagcagg
     3121 ttcgcctggc cggcgacagc gcggatagtt ccggtggcat caaatactgc cgtcatcacc
     3181 agcgccagaa cgcttggcag gactacagga ttcagcgcgc ccataatgtc caggctgcca
     3241 atcagtgaat tgccgttttc atcgctcaat gaaggcatgg cgaaaacgcc ggagaaatgg
     3301 acgttaggat cgaagatcaa accgacaatt gagataccga taatggtcag cagaatgcca
     3361 ccagggactt tcagtttttc caagccgatg atcaccgcca gacctgccag tgacataatc
     3421 accgggaagg tcgcgaaatc acccagcgca acgggcagac catcaagcgg gtttttaatc
     3481 accagaccga caccattagc ggcaatgagc agcaggaaca gaccgatacc gatccccgtg
     3541 ccgtgcgcca caccgtgcgg caagttgcgc aaaatccagc tacggatacc cgtggcagaa
     3601 attaccgtaa acagcacacc catcaggaac acggcaccca gcgcgacagg tacgctaata
     3661 tgttgcccca gcaccaggct gaatgcggta aacgccgtca gggagatggc gcaaccaatc
     3721 gccaacggca gattagccca cagacccatc acgatagaac cgagtccggc aaccagacag
     3781 gttgcaacga aaactgccgc aggcgggaag cctgctttac ccaacatacc tggaacgacg
     3841 atgaccgagt agaccatcgc cagaaacgtt gttaacccgg caaccacttc ctgacggaca
     3901 gtgcttccac gttgtgaaat tttaaaccag gcgtcgagtg aaccgccggt acgcgctgat
     3961 ggcgtagaca taagaaaaca tcccctgaga gtttaatttt cgtcaagatg cgtggcggac
     4021 aatcccctgc cagcgaaacg ttatcttcct gctccaggtt tgcggcaatt tggtgcgtcg
     4081 caaaaaagca aacgtttagc tccgcgcata caaaacgggt ggtaaaaaaa agcaaacgat
     4141 tatccggcga tcatagcggc tttttcaacc gaagtttgct gcttttttct cttttattgt
     4201 cttgacgatg aaaaaccagg caattgatgc gcaaacgtta tcgtcgatgt gcaagattgc
     4261 agcgttatcg agccgtatac cgttctggat tgcgataaaa aatcgtctga gatgacaaaa
     4321 atattgcggc taaaaagtat acttttacac tggcgaagaa acgtcggtga catacaatta
     4381 aagcatcaac accaaccgga acctccacca cgtgctcgaa tgaggtgtgt tgacgtcggg
     4441 ggaaaccctc ctgtgttcca gcgggataga gagaaagaca aagaccggaa aacaaactaa
     4501 agcgcccttg tggcgcttta gttttgtgtt tgcggataat tccaaaagag ctcgatcagt
     4561 cccctcgccc cttcggggag agggttaggg tgaggggaac aggcagcacc agtgcaaata
     4621 tttaccctca ccccggcccc atagttaacg atgagtcagc ttgtccttgt ggcgctttag
     4681 ttttgttcat cttccagcaa gcgtgcgccg gtaccttctt ctcctaagcg gtcgcccggg
     4741 ttacgcaacg ggcaatcact gcgcgaaagg cagccacaac caatacatcc gtccagttcg
     4801 tcacgcagcg ccactaaggt atgaatgcgc cgatccaact cttctcgcca ttgggacgaa
     4861 agctgtttcc actctttcgc acttaacgta tgcccttctg gcaacacgcc aaacgcttca
     4921 ccaatggtcg ccagcggaat gccaatacgc tgagcgattt tgataattgc gacatatcgc
     4981 aacacatcac gtttatatcg ccgctgattg ccgctgttac ggatactagt aatcaaccct
     5041 ttgctttcat agaaatgcag cgccgatacc gccacaccgc tgcgtttcgc cacttcgcca
     5101 ggggttagca gcgctttaat gcggggtaat ttcttttcca taaatcgctt tacctcaagt
     5161 taacttgagg aattatactc cccaacagat gaattaacga actgaacact gaaaagaggc
     5221 agatttatgt cccatcagaa aattattcag gatcttatcg catggattga cgtgcatatt
     5281 gaccagccgc ttaacattga tgtcgtcgca aaaaaatcag gctattcaaa gtggtacttg
     5341 caacggatgt tccgcacggt gacgcatcag acgcttggcg attacattcg ccaacgccgc
     5401 ctgttactgg ccgccgttga gttgcgcacc accgagcgtc cgatttttga tatcgcaatg
     5461 gacctgggtt atgtctcgca gcagaccttc tcccgcgttt tccgtcggca gtttgatcgc
     5521 actcccagcg attatcgcca ccgcctgtag ttttattgcc cgcgcgttaa ctcccgcgcg
     5581 ggtaattgct ccatccattg cataaacacc tgcggcggca tcgccttcgc aaaaaaccat
     5641 ccctggcaat agcgcacgcc gcgtttgcgc agccagttaa cctgctcctc agtttcgacg
     5701 ccttcagcga tcgtttttaa ccccaggctg tgcgccagct cgatgatgtg ttccgcaatc
     5761 aaatgactgg ttttgtgggt ggtcagcgtt tcaacaaacg atttatcgat tttcaaaata
     5821 tcgacattca atgatttaag gttatgcaag ttagagtagc caataccaaa atcatcaatt
     5881 gccacttcgt aaccagcctg gcggaaagcc agaattaatc ggcgtcattt tttcgacatc
     5941 aagaaatgca tgctcagtca cttcaaattt aatttgctgc ggacgcaccg cgtattgctc
     6001 tgttttctga ttgattcgcg ctatcaaccg tgacgtatga aaatcggagg ccgacaggtt
     6061 aatagaaaca tagcgatcgg catgtgttgc caggtacgcg cccagatcgc ggaagacatt
     6121 atcaataaca taatcagtta tctgttctat catcccctcc ttttctgcca gcggaataaa
     6181 ctctgccgga ttcattattt gcccctgctc accaggccaa cgtaacaacg cttcagcgcc
     6241 gatacatttt tctgttttga tatcgattat tggctggtaa taaagacaaa gttgatgttt
     6301 ttcgagggcg cgttgcaatt tacgtttggg agataaatag ttttgtcgaa tacgtagcca
     6361 gagtaatagc agaacaagac tccccaaaat accggcgggc aacgcaaaaa taagatgatt
     6421 ataaaaatgg gttataagac gttgatatga agtcgcaaca atggctgcaa ttgggcgttt
     6481 tgtcgaataa attgtcgcat ataaatagcc atttctttgt acggttaaat ccttcaaatg
     6541 aatcagcgga gaaaacgttg ctgccgaggc ctctttgctt aacgagaaaa aggttttcat
     6601 caccgtatca tacacacccc attgcaatgt cgggtcatca gacatcactt cactccagaa
     6661 gagagggttg ataaccgcca cataatttcc ccgctgcata taggtcattt tatagccaga
     6721 gaaaaaaggc gtatcgcggt aataatagat agaaacgtta ggttcacgct tataatcggc
     6781 cggtgcaatc gtatagccgt ttacaggcgc tatcagcgat gagcataaaa aatggttatc
     6841 acgaattcat taatataaag atagccacga ataatattca acattcgctt ttgatgggct
     6901 ggagtgcaaa cttgcccctg atagcgttca gctgcatcgc tcactaaatc tgcctggcga
     6961 atgaccagct cggatttatc cagtgccagt tgagcaaagg ttcggagctg attattcact
     7021 tctgattttg cccaaaggaa ggcaatccat agcgaaagaa tgatgggaaa gagaactaaa
     7081 aagataatgc ccggcaacgc cagtaattgg tgtcgtgcac gatgactcat tgctatcaat
     7141 ttcctgcctg tttatacccg gccattgcaa ggtgaaaccc tggctaccga cgtctttaga
     7201 taaatcttac ggtataaata gcacttttgc ctgatttgaa attgatacct ttttaatcgt
     7261 cacctggatg aaaataattc attgatattc aaaattaatt ttctacagga atacagaggc
     7321 ggcgggaaga tatgccctta atgccactga aaaaccacat aatttgtaca gcaataaaag
     7381 tcacggccta atatggctaa caaacaatca attgtgatat agttcacaaa attaatgaaa
     7441 caaacagagt gtttcatttt tgtgttctgc aagccccgcg atagccagag tgtaccacgc
     7501 ctcccgtgaa caacgccgcg ctgtccaggg catcggctct tttcagagga attgtttgat
     7561 ggctaccctc accactggcg tggttcttct tcgctggcaa cttcttagtg ccgtaatgat
     7621 gtttctggcc agcacactca acatccgttt tcgtcggtct gattatgtcg ggcttgcggt
     7681 gatcagcagc ggtctgggcg ttgtttccgc ctgttggttc gcaatggggt tgcttggcat
     7741 cacaatggcg gacatcaccg ctatctggca caacatcgag tcggtgatga tagaagagat
     7801 gaatcagaca ccgccacaat ggccaatgat tttgacctga tacgtagaa
//
LOCUS       DAZPCS010000163         7767 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZPCS010000163 DAZPCS010000000
VERSION     DAZPCS010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7767
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.165"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            160..378
                     /locus_tag="ACHUQ7_003401"
     CDS             160..378
                     /locus_tag="ACHUQ7_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFR1136667.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            596..838
                     /gene="yiiF"
                     /locus_tag="ACHUQ7_003402"
     CDS             596..838
                     /gene="yiiF"
                     /locus_tag="ACHUQ7_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YiiF"
                     /protein_id="HFR1136668.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(1168..2097)
                     /gene="fdhE"
                     /locus_tag="ACHUQ7_003403"
     CDS             complement(1168..2097)
                     /gene="fdhE"
                     /locus_tag="ACHUQ7_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFR1136669.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQKVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(2094..2729)
                     /gene="fdoI"
                     /locus_tag="ACHUQ7_003404"
     CDS             complement(2094..2729)
                     /gene="fdoI"
                     /locus_tag="ACHUQ7_003404"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFR1136670.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2726..3628)
                     /gene="fdoH"
                     /locus_tag="ACHUQ7_003405"
     CDS             complement(2726..3628)
                     /gene="fdoH"
                     /locus_tag="ACHUQ7_003405"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFR1136671.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3641..6691)
                     /gene="fdnG"
                     /locus_tag="ACHUQ7_003406"
     CDS             complement(3641..6691)
                     /gene="fdnG"
                     /locus_tag="ACHUQ7_003406"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(6104..6106),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFR1136672.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            6885..7718
                     /gene="fdhD"
                     /locus_tag="ACHUQ7_003407"
     CDS             6885..7718
                     /gene="fdhD"
                     /locus_tag="ACHUQ7_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFR1136673.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGRLTGCTHVAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         1793 a         2000 c         2071 g         1903 t
ORIGIN      
        1 atttttgagt aataaatgaa acaactgcaa cgctcttatt atttctggaa aacgcctcgc
       61 aaaaacaaaa tgacatgcaa aaatccgcat ttttttaaat gcttttccgg cgtaattttc
      121 gcacatacat taatgagtaa tatatgtaag gtggtggtaa tggcgatgaa tacggttttt
      181 cttcatttat cagaagaggc aattaaacgg ctgaacaagc ttcggggctg gcgtaaggtt
      241 tcacgatctg caattttacg cgaagcggtt gagcaatatc tggagcggca gcaatttccg
      301 gtgcgtaaag caaagggagg caggcaaaag ggggaggtag ttggtgttga tgatcagtgt
      361 aaggagcata aggaatgaaa aaaatttttc attcttgaat ataaaaaaca gatgccctta
      421 ttctggtatt aatacaaggc tgttttactt gaacttataa taactgcaac tgttacatcg
      481 tatctggaaa acgcctcgca aaaataaaaa atgatgcgta aatgagcttt ctttaaagat
      541 tcggcaaggg tacttttctc tccatagatt caaaaatgat attggcgaga tatttatgaa
      601 ctctctggca ggtatagata tgggcagaat tttactcgat ttatcgaatg aggtgattaa
      661 gcaacttgat gatctcgagg tgcagcgtaa tcttcctcgt gcggacctat taagggaagc
      721 ggtagatcaa tacctgataa atcaatcgca aacagcaaga accagtgttc ctggcatctg
      781 gcaagggtgt gaggaagatg gtgtcgaata tcagcgtaag ctgcgcgagg aatggtaatg
      841 ggaaaaaatg atgtcaatca gattgctgac aacgtgcgcg ttgttcatgc cggatgcggc
      901 gtgaacgcct tatccggcct acaaagtcat gcaaattcaa tatattgaag tttattggta
      961 ggcctgataa gcgcagcgca tcaggcaatc atacgcttgt catcagtgac atacccataa
     1021 ccttatcgat gtgttggagg gaagaacgag gcaaacagac aattgcggca tgatcttgaa
     1081 tgggtaacgc ggaatacgag agatggtgtc gtcacaccct atgagctgta atccgggcga
     1141 cctgcgccac ccggaaaacg tcagagatta ctctccttca cccggaaaca ggaacgggtt
     1201 aatggaacta cgggcgtagc cttcttgctc cattcgcgcg tccagtacca gagaggcgag
     1261 gtcatctgcc acggcttcaa ctttcggttc tttttcctga taaagaatct tcaggtaagt
     1321 gccgcagtca tcgcagcttt cggctttaat cgcggcctgt tcgtcatcca gcgaccagta
     1381 atgcagtttg ccgctctgct cgcagttgct gcattttacg cgcactacgt gccattcggt
     1441 ttcacacagg ttgcagtgca ggtaacgcag accctgagtg gtgccaattt gcaccatgct
     1501 ggacaccggc atagagccac ataccgggca atattgacgt tgttcgccgt attcagcgcg
     1561 ggctttgccg gggatcagat tggccatctg cgcccagtag agcgacagcg cagcccagat
     1621 aaacggcgct ttatcgctgc tgacggacga gaaatcagag gcaaacagcg cgctggccat
     1681 atcttccagc tcctgagtcg atgccttctc cagattctca atcactgcca gcgccgggcc
     1741 gctcatttca ggtttcagct cagcaattag cgccatcagc agcttttgcc agtgcttatc
     1801 acgcggcaga acgtgaatat ccagcggggg cttgccctgt gcgctggctt ctttaatgcg
     1861 cgcggtcaga tccatctcca gcggatggtc gtacagcacc actttctggg cgtgggcgat
     1921 aagcgcagca aagcgcaggt aatcacccag cggattattt tctgccagct cgcgcagacg
     1981 ctcggcgcgg cggttgtata aattcttgag ccgagggaac aataacggcg gaatcatatc
     2041 cgccgtacgt ttctcgctcg aacccagctc atcttgcggg attatgcgaa tactcattca
     2101 gctttctttt ccgttgtctt gcggacctca cggtaccagc gcgggtgatg tttcttcgcc
     2161 caggcgctgg taacccatcc ttccaccatg gcggtaatcg tgcctttcac ccaaagggcg
     2221 gcgtagatat gcaccatgat aaccacaatt aacgccactg cggcaaatga atgcagcatt
     2281 aacgcgaatc ggatcaccgg gattgagaaa gcaggcgcaa aataaggacg ccagataatc
     2341 acgccgctca ccagcagcag aaccaggaaa ataatcgccg cccagaaaac gcatttctga
     2401 ccgaagttat aacgcccggt gtcacctact tcctcgttga cgacgatctt acgaatattc
     2461 ttcgcccaaa agatatcatc ccgattgatt aggttttgat gccagtaacg gaaaaacatg
     2521 atgatgaacg aggcaaacat aaccacgccg acaaacgggt gcagaattcg cgccagctgc
     2581 ggtgtgccca tgatttgcat caaccagttg aaggacggga acaaaaagcc cagcccgctc
     2641 accgccgcca ggatgaagca gaaggcggtg atccagtggt tgatacgttc cggcgcggtg
     2701 tagcgcacga tggtgtcacg tcgtttcatt tgcgctcctc gtctttctct tcgtgcagat
     2761 tattctcttc ctcatccgca cggttcggac cgacacccac gtagtggaag atactggccg
     2821 caaaggtagc cgcaaagcca acagccgcga gcggtttcca gatgcctttc cagaatttca
     2881 cggtttcgct gatttccggg ttctccggca agccatgata cagatttggc ttgtcagcat
     2941 ggtgcagcac gtacatgacg tgtgtaccac cgacgcctgc cggatcgtac agacccgcat
     3001 tgtcgtaacc gcgggttttc agctcagcaa cgcgctcgct cgccagcgtt ttcatcgact
     3061 ctttcgtgcc gaagtgaatc gcgcccgttg ggcaggtctt cacgcaggct ggttcttgcc
     3121 caaccaccac gcggtcaacg cacagcgtac atttgtagac gcggttgtct tccgggttga
     3181 ggcgcggaat gtcgaacgga cagcccgcaa tgcaataacc gcagccaatg cactgctcgg
     3241 actggaagtc gacgataccg ttggcatact gaataattgc accttccgcc gggcacgctt
     3301 tcaggcagcc tggatcggaa cagtgcatac agccgtcttt gcggatcagc cattccagtt
     3361 tgtcgttctg ctccacttcc gagaagcgca ttaccgtcca cgatttggcg cttaaatcat
     3421 tggggttgtc gtacacccca atgttattgc cgacggtatc gcgaatgtcg ttccactctg
     3481 aacacgccac ctgacaggct ttacagccga tacaggtggt aacgtcgatg agtttcgcca
     3541 cttcttcctg gaagtcccgc gcctgaggcg cgggggtcag accgttagtc gcggaacgac
     3601 gaatgatatc ttgagattga taagccatat gtcgtctccg ttacaccttt tccacattca
     3661 caaggaagga cttaaactcc ggcgtctgcg tgttcgcatc accaacgaat ggcgttaaag
     3721 tattggcaat aaagcctttt ttagctacgc cttcatagcc ccagtgaatc ggaataccga
     3781 tggtatcgat atctttgccg ttcgctttca gcgtgcgaat acgtttggtc accaccgctt
     3841 tggctttgat atagccacgg ttggaggaga ctttcacggt atcgccctgg gcaatgccaa
     3901 gtttattcgc cagcgactcc ccgatttcca caaactgctc tggctgcaaa atcgcgttca
     3961 acagcgcgtg tttggtccag tagtggaagt gctcggtcag acgataggtg gttccgacat
     4021 acgggaactt atcggcttta cccaatgctt cggcgtcgtc tttaaagata cgtgcagctg
     4081 ggttcgagat aacgtttgga tgcagcgggt tagttcccag cggcgtttca aacggctcgt
     4141 agtgttccgg gaacggacct tccgccatct tatcgagggc aaacagacgc cccatgcctt
     4201 cctgctgcat gataaacggc ccgacgccgc tacccggagg cgctgcgctg tagtccggaa
     4261 tatcccagcc ggtccactta gtgccgtccc atttcagcaa ctgacgcttc ggatcccacg
     4321 ggttaccctg cggatctgcg gaggcgcggt tatacagaat gcggcggtta agcggccatg
     4381 cccatgccca gcccagcgtg ttaccgaggc cagacggatc ggcgttatca cgacgcgcca
     4441 tctggttgcc ttccggcgtc cagctaccgg cgaaaatcca gcagccacag gaggttgtac
     4501 cgtcatcgcg caattgggcg aacgagctaa gttgttggcc tttcttgacg ataaccgcac
     4561 cggttgccgg atcggtaata tcggccagcg ccttaccgtt gctctccatc gctacttctt
     4621 ccgacttcgg ctcatgcgga atggcgtagt tccaggtcat gttcagcacc tgatccgggt
     4681 tcgcgccacc ctgttcggca tacatcttgc gcaagcgcag gaagataccg gagaggatct
     4741 caccgtcagt cagcgcaatc cccggggcgt ccgcaccttt ccagtgccac tgcaaccagc
     4801 gacctgagtt aacgattgaa ccgttctctt ctgcgaagca ggtcgatggc agacggaaca
     4861 cttcggtctg gatcttcgac gagtcaactt cgttcagctc accgtggttc tgccagaagt
     4921 tagaggtttc agtgttcagt gggtcgatgg tgacgaggaa cttcagtttc gacagacaac
     4981 cgatcacttt gtttttgttc gggaatgagg caacagggtt aaagccctgg cagatatagc
     5041 cattgacctt gccctctttc atcatctcga agtattgcag gacgtcgtag cctttatccc
     5101 acttcggcaa ccagtcaaag ccccagctat tttccgccgt cgctttatca ccaaagaagg
     5161 ccttcatcat agagacgaag aatttcgggt agttgcccca gtagtttacc tggccttcca
     5221 gcagcggttt tggcgtgttg gcggtaaggt aggtttgcag atcggtctgc ttctcgcttg
     5281 gcagcgtcat gtaacctggc agactctgcg acagtagccc caggtccgtc agcccctgaa
     5341 tattggagtg accgcgcagg gcgttaacgc cgccgccggc catccccata ttgccgagca
     5401 gcagctggat catcgccatc gtacgaatgt tttgcgcacc aacggagtgt tgcgtccagc
     5461 cgagggcata caggaacgag gcagttttat cgtgagcact ggtttctgcg atgtattcgc
     5521 agactttcag gaacgcgtct tttggcgtac cacagatgtt ttcaaccaca tctggcgtgt
     5581 agcgggaaac gtgctgtttc agcaagttcc acacgcagcg cggatgttgc agcgtggtat
     5641 cgcgtttggc gaagccgttt tcgtccagtt cataagtcca ggaggattta tcgtacttgc
     5701 gtttttccgc gtcgtagccg gtgaacaggc catcttcaaa gccgtaatcc tcacgcacga
     5761 tcaggctggc gttggtatag gcttcggtgt attcgtggtt gaatttttca ttgttcagca
     5821 ggtacagcaa tacgcctgac aggaaagcaa tgtcagtacc ggaacgaata ggggcatagt
     5881 agtcagctac cgccgccgta cgcgtaaagc gaggatcgat cacaatcagc ttcgcgccgt
     5941 tgtgaatttt ggcttccatc gcccagcgga acccgaccgg gtgagcttca gcggcgttac
     6001 cgcccatcac cacgacgagg ttggcgttct tgatgtcgac ccagtggttg gtcatcgcac
     6061 cgcgaccaaa tgttggagca agacttgcta ccgttggtcc gtgtcagaca cgcgcctggt
     6121 tgtcgaccgc gagcataccc agcgcgcggg agaatttttg cgttaaatag ccggtttcgt
     6181 tactcgacgc ggaagcacac agcatcccgg tggagagcca gcggttaaca gtcacgcctt
     6241 cggcgttttg cgcaatgtag ttagcatcgc ggtcttcttt catcagtttg gcgatgcgat
     6301 caaacgcctc ttcccaactg atttgctgcc atttatcaga acctggcgca cggtattccg
     6361 gaaacttcag acggctttcg gagtggatga aatccaccag gccagcgcct ttcggacaaa
     6421 gtgcaccgcg gttgaccggg tgatccggat cgccttcgat atggaagata gatgctttgg
     6481 cgttttttgc tccgtcaccg aggctgtaca tcaacagccc acagcctacg gaacaatagg
     6541 tgcaggtatt acgggtttcg cgggtgcgca gcagtttata ctgccgggtt tccgcgagtg
     6601 ctacgctggg tgcaaaaccc agtgccgctg ccgtggtgcc tgccataccg ccagcgcaga
     6661 tcttaaagaa ctgccttctg ctgacctgca tggattgctc cttgtttcga cattgtcacg
     6721 tcccatttac attcgcttgc tgcgtgtgca gggagtggga gttatttttc tttgcggaag
     6781 gggccgcaaa ggtccagaat tggctcaatt tccctccatc taggaagggt ttgtaacaga
     6841 ataccataat gttggtgtgt atgttcttat ctggttaaga gaaagtgaaa aaaacacagc
     6901 gaaaagaaat cgaaaatgtg acaaatatca caggtgttcg tcaaattgag ttatggcggc
     6961 gtgatgattt acaacaccca cggttagatg aggtcgcgga agaagttccc gttgcgctgg
     7021 tctacaacgg catttcgcat gtggtgatga tggcgtcgcc caaagacctt gagtactttg
     7081 cgctcggttt ttcgctttcc gaggggataa tagaaagtcc gcgcgatatc ttcggcatgg
     7141 atgtcgttcc ttcctgtaat ggtcttgaag tacaaattga gctttccagc cgccgcttta
     7201 tggggttgaa ggagcgccgc cgggcgctgg cgggacgtac gggctgtggc gtatgcggcg
     7261 tggagcaact taatgacatc ggaaaaccgg tgcagccgct accgttcacc cagacgtttg
     7321 atctcaacaa actggatgat gcattacgtc atctcaacga ttttcagcca gtggggcgac
     7381 tgactggttg tactcacgtc gctgcctgga tgttgccatc tggcgaactg gtcggcgggc
     7441 atgaagacgt gggtcgccat gtggcgctgg acaaactgtt aggccgccgg tcacaagaag
     7501 gggaaagctg gcagcaaggt gcggtactgg tttccagtcg tgccagttat gaaatggtgc
     7561 aaaagtcggc gatgtgcggc gtagagattt tgtttgcggt gtctgccgcg accacgcttg
     7621 ctgtagaagt ggctgagcgc tgtaatctga cactggtagg tttttgtaaa ccgggtcggg
     7681 caacggttta tacccatccg cagcgtttga gcaattaatt aaaataagtc ctgcgatata
     7741 tattaaattg caggacttat tcatttc
//
LOCUS       DAZPCS010000164         7746 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZPCS010000164 DAZPCS010000000
VERSION     DAZPCS010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7746
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.166"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..1015
                     /gene="ydfJ"
                     /locus_tag="ACHUQ7_003408"
     CDS             <1..1015
                     /gene="ydfJ"
                     /locus_tag="ACHUQ7_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HFR1136674.1"
                     /translation="ILLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVAMG
                     TNCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVFEK
                     VNDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNKAI
                     PTDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYAPS
                     TIMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPILAG
                     IFCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVRAAQPS
                     RSL"
     gene            1104..2564
                     /locus_tag="ACHUQ7_003409"
     CDS             1104..2564
                     /locus_tag="ACHUQ7_003409"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFR1136675.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLEHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGMMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(2600..2803)
                     /gene="ydfZ"
                     /locus_tag="ACHUQ7_003410"
     CDS             complement(2600..2803)
                     /gene="ydfZ"
                     /locus_tag="ACHUQ7_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFR1136676.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(2981..3667)
                     /gene="rspR"
                     /locus_tag="ACHUQ7_003411"
                     /gene_synonym="ydfH"
     CDS             complement(2981..3667)
                     /gene="rspR"
                     /locus_tag="ACHUQ7_003411"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFR1136677.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(3756..4502)
                     /gene="ydfG"
                     /locus_tag="ACHUQ7_003412"
     CDS             complement(3756..4502)
                     /gene="ydfG"
                     /locus_tag="ACHUQ7_003412"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFR1136678.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELTDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            4639..6684
                     /gene="dcp"
                     /locus_tag="ACHUQ7_003413"
     CDS             4639..6684
                     /gene="dcp"
                     /locus_tag="ACHUQ7_003413"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFR1136679.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELITRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(6729..7247)
                     /locus_tag="ACHUQ7_003414"
     CDS             complement(6729..7247)
                     /locus_tag="ACHUQ7_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HFR1136680.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            7523..>7746
                     /gene="ydeI"
                     /locus_tag="ACHUQ7_003415"
     CDS             7523..>7746
                     /gene="ydeI"
                     /locus_tag="ACHUQ7_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HFR1136681.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKG"
BASE COUNT         1941 a         1813 c         1957 g         2035 t
ORIGIN      
        1 catcttgctg gtgacgctgc gtattattca gggattgggt gcaggtgcgg aaatttccgg
       61 tgccggtacg atgctggcgg aatatgcgcc aaaaggtaag cgcggaatta tctcctcatt
      121 tgtggctatg ggaactaact gcggcacctt aagcgcaacg gcaatctggg cctttatgtt
      181 cttcattctc agtaaagagg aactgctggc gtggggatgg cgtataccgt tcctggcgag
      241 cgttgtcgtg atggtctttg ctatctggtt gcgtatgaat ctgaaagaaa gcccggtctt
      301 tgagaaggtt aacgacagca accaaccgac agcaaaacct gcacctgctg gtagcatgtt
      361 ccagagcaaa tccttctggc tggcaacagg gctgcgtttt ggtcaggcgg gtaactccgg
      421 tttaattcag actttccttg caggctattt agtacagacg ttattgttta acaaagcaat
      481 tccaacagat gcattgatga tcagttcgat tctcggcttt atgaccattc cgttccttgg
      541 ttggttatcc gataaaattg gtcgccggat cccgtatatt attatgaata cctctgcgat
      601 tgtgctggcg tggccaatgc tttctatcat cgtagataaa agctatgccc cgagcaccat
      661 tatggttgca ctgattgtga ttcataactg tgcggtgctg ggattatttg ctctggaaaa
      721 cattaccatg gcagaaatgt tcggctgtaa aaaccgcttc acccggatgg ctatttcaaa
      781 agaaattggt ggtcttatcg cttccggttt tggtcctatc ctggcgggta ttttctgcac
      841 catgacggaa tcctggtatc cgatcgccat tatgatcatg gcatattcag tgattggttt
      901 aatctctgcg ctgaaaatgc cagaggtgaa agaccgtgat ttaagtgcgc tggaagacgc
      961 tgcggaagat caaccgcgtg ttgtaagagc tgcgcaacct tccagaagtc tgtaaaccct
     1021 caatcccttc tcttaccgga gaggggattt ttattcatat aacaaaacat atagcttgcc
     1081 atatttaaat ttaaggaatt gttatgggaa ataatttgtt atcagcaaaa gcgacactcc
     1141 ctgtttatga tcgtaataac ctggccccaa gaattgttca tttaggcttt ggtgcatttc
     1201 accgtgcgca tcagggcgtg tatgccgata ttcttgctac agaacatttc agtgactggg
     1261 gatattatga ggtcaactta atcggcggcg aacagcaaat tgccgattta caacagcaag
     1321 ataatcttta taccgttgcg gaaatgtcgg ccgatgcgtg gacggctcgc gtcgttggcg
     1381 tcgttaaaaa agccttgcac gtacagatgg atggcttaga aaccgtgttg gctgcgatgt
     1441 gtgaaccgca aatcgcgatt gtctctctga caatcaccga aaaagggtat ttccactctc
     1501 cggcgaccgg acagttaatg ctcgagcacc cgatggtcgc tgccgacgtg caaaatcccc
     1561 accagccgaa aacagcaaca ggggtgattg ttgaggcgct ggctcgccgt aaagcggcag
     1621 gacttcccgc atttaccgtc atgtcatgtg acaacatgcc agaaaacggt catgttatgc
     1681 gtgacgttgt cacttcctac gcgcaagccg ttgatgtaaa actggcacaa tggatcgaag
     1741 ataacgtgac tttcccatca acaatggtgg accgtattgt gcccgcagtg acagaggata
     1801 cgctggcgaa aatcgaacaa cttaccggtg tgcgcgatcc tgctggcgtt gcctgtgaac
     1861 ctttccgcca gtgggttatc gaagataact ttgtagccgg acgtccggaa tgggaaaaag
     1921 cgggagccga actggttagt gatgtgctgc cttatgaaga gatgaagttg cgcatgctca
     1981 acggcagtca ttcattcctg gcatatctgg gttatcttgc tggatatcag cacattaatg
     2041 actgtatgga agatgaacat tatcgtcatg cggcgtatgg catgatgttg caggaacaag
     2101 cgccgacgct gaaagtgcag ggcgttgatt tgcaagatta cgctaaccga ttaattgcac
     2161 gctatagcaa cccggcgtta cgtcatcgaa cctggcagat tgcgatggat ggcagccaga
     2221 aattgccaca gcggatgttg gattctgttc gctggcatct ggcgcatgac agcaagttcg
     2281 atctgctggc gctgggcgtc gcgggttgga tgcgttatgt cggtggtgtt gatgaacagg
     2341 gaaatccgat agaaatcagt gacccactgt tacctgttat tcagaaggct gtacaaagta
     2401 gtgccgaagg gaaagcgcgc gtccagtcat tgctggcgat taaggcaatc tttggtgatg
     2461 atttgccaga caatagcttg tttactgcaa aagtgacgga agcgtacttg tctttattag
     2521 cgcatggcgc gaaagcgacc gtggcgaaat attccgtgaa gtaaaaaaag caatgccgcc
     2581 atcggcggca ttcacacact tagttcatgc cgagacgaat caggtccaga ggtgccagtt
     2641 tctcttcaca accttcaaca actacggttt ttccgcggcg gatttgctca gcggtcagac
     2701 cttcagtatc aatcgacagg atcttgccag tgtgaccggt gccgctaacc ataacacggc
     2761 tgccagtggt gattgcgtta cggttacgat cgtaggtcgt cataggtgtt ttctcctttc
     2821 tgatttacag tcgaagccca cgtaacgggc gcaatataaa tactcctgtc gagtgaaata
     2881 ttttttgttt ttgatcaagc tcacatcttt tttgatgcat gcatgagccg taaggcgggg
     2941 ggaaaagcct ctcatcccgg atgggatgag agtaaatgaa ttactcttcg ctaaaccagt
     3001 cgctgttttc ctggcggatc tggcgcacgg attcgctgat ttcctgcaaa tgttgcgtca
     3061 ttgcacgttc taccgcatcg ccatcacgtt tttgcagggc agagaaaata tcaagatgct
     3121 ggcgtaacag catttctggt ggagaaacat ggtcgaaact catatagcgc acgcgatcaa
     3181 cggtcgcttt caggttctca atggtatccc acgccagttg acagtcggca atctgcgtca
     3241 ggagttgatg gaagttgtca tcaagttcaa aaaaatcatc cagttgcttg cgctcaatgg
     3301 caatgcgttg ctggtgaaga ttttgttcca gttggtagca ctggctttcg gtaatcatgc
     3361 tcgccgcccg acgcgccacc gcgcactcaa tggcctgacg gataaaactg ccgttgcgca
     3421 cctgggccat ggaaattttg ttgacgtagc tgccacgctg cggacgaatt tgaatcaggc
     3481 cgttttccgc cagtttaata aaggcttcac gaaccggctg gcgtgacaca ttgaaacgaa
     3541 cagaaacttc tttttccgac aacggtgtgc ctggtgcaat caggcaatgg acaatgtcgc
     3601 gacgaagaat acgataaatc tgctgattga caggctgtgt gggattaagt tgcgtttcga
     3661 cggtcattcg ttcttgcttg tgagtgagtt aactgcatga gtctaccact tttttgtgac
     3721 aagcaataac ccggcagtta cgccgggtat aaaaattact gacggtggac attcagtccg
     3781 gcatagcttt gggtaaccgg catcatttcc agggtattga tattgacgtg agcaggcagc
     3841 gttgacaccc accagacggc ttcgctgaca tcttctggcg tcaatgcaac ggtattttga
     3901 taggtttttt ccgctttacc gtcatcgcct ttaaagcgga cattggaaaa ctcggtgcca
     3961 cccaccagac ccggttcgat gtcggtgacg cgcaccgccg taccatgcag atccgtacgc
     4021 agattcaggc taaactgacg aacaaacgct ttcgtcgcac cgtaaacgtt accaccggca
     4081 tacggccagc tgcctgccgt tgagccaatg ttaataatat gaccatgatt acgttcaacc
     4141 atacccggta agacggcgcg cgtcatatat actaggcctt tgttgttggt atcaatcatc
     4201 gtttcccagt cttcaacgct ggctttatgc gcaggctcca tgcccaacgc caggccggca
     4261 ttatttacca ggatatcaat attgctccac tcggcaggga gcgatgccag catctcttca
     4321 atagcggcgc ggttgcgaac gtccagttgg gcgatataca gattatctcc cagttcgtct
     4381 gttaactcct gcaaccgctc ctggcgacgg ccagtggcga taactttatg cccttgttga
     4441 ataaaacgac gagtaatgca ttcaccaaaa cctgccgttg ctccagttac taaaacgatc
     4501 atctcactgt tcctcaacgc ttttgtgtgt caataacata gcacgtgatg cttaagagcg
     4561 ggtaatactg gtttgcaaga ttgcactccg ttatcagtaa gcctacgcta tggaacacct
     4621 cgcgttcagg agaagaaaat gacaacaatg aatcctttcc ttgtgcaaag cacactgccg
     4681 tatctggctc ctcattttga tcaaattgcc aatcatcact atcgcccggc attcgatgag
     4741 ggaatgcaac aaaagcgggc agaaattgct gccatcgcgc ttaacccgca aacgcctgat
     4801 ttcaacaata ctattctggc actggaacaa agcggagaat taattacccg cgttaccagc
     4861 gtcttttttg cgatgactgc ggcgcatacc aatgatgaat tacagcgtct tgatgaacag
     4921 ttttccgctg aactggcgga actggctaat gatatctatc tgaacggtga attattcgcg
     4981 cgggtagatg ctgtctggca gcgccgtgaa tccctggggc ttgatagtga atccatccgc
     5041 ctggtggagg ttattcatca acgttttgtc cttgccggag ccaaacttgc acaagctgat
     5101 aaagcaaaat taaaagtact gaatacagaa gctgcgaccc tgaccagcca gtttaaccag
     5161 cggttacttg cagcaaataa atccggcggt ctggttgtga acgatatcgc gcagctggca
     5221 ggaatgagtg agcaagagat tgcgctggcg gcagaggcgg ctcgcgagaa aggtctggat
     5281 aacaaatggc tgattccgct gctgaatacc acccaacaac cggcgcttgc cgaaatgcgc
     5341 gatcgtgcga cgcgtgaaaa actgtttatt gcgggctgga cgcgagcgga aaaaaatgat
     5401 gccaatgata cccgcgctat cattcaacgt ctggtagaga ttcgcgcaca gcaggcgaaa
     5461 ctgcttggtt ttcctcatta tgccgcatgg aaaatcgccg atcagatggc aaaaacgcca
     5521 gaagcagcac tcaactttat gcgggaaatt gttccagcgg cgcgtcaacg tgcgagcgat
     5581 gaattagcct ccatacaggc ggttatcgat aagcagcagg gcgggtttag cgcgcagccg
     5641 tgggactggg cattttatgc ggaacaggta cggcgggaga aatttgatct tgatgaggcg
     5701 cagctcaagc catattttga attaaacacg gtgttaaatg aaggtgtatt ctggaccgcg
     5761 aatcagctct tcggtattaa gtttgtcgaa cgttttgata ttccggtcta ccatcctgac
     5821 gttcgtgtgt gggaaatttt tgatcataat ggcgtgggac tggcgttatt ttacggtgat
     5881 ttcttcgccc gtgattcaaa aagcggcggt gcatggatgg gcaattttgt tgagcaatca
     5941 acgcttaatg aaacgcatcc ggtaatttat aacgtctgca attatcagaa acccgctgcc
     6001 ggtgagcctg cgttgttact ctgggatgat gtaataacct tattccatga atttggtcat
     6061 acgctgcacg gcctttttgc ccgccagcgt tatgccacgc tttccggcac caacacgccg
     6121 cgtgattttg tcgaatttcc gtcgcaaatc aacgaacact gggcaacgca tccgcaggta
     6181 ttcgctcgct acgcccggca ttatcagagc ggggcagcaa tgcctgacga actgcaacag
     6241 aaaatgcgta atgccagcct gttcaacaaa gggtatgaga tgagcgaact gcttagcgcc
     6301 gcacttctcg atatgcgctg gcattgcctg gaagaaaacg aagcaatgca ggatgtcgat
     6361 gattttgaat tgcgggcgct ggtggcggaa aatatggatc ttcctgctat accgccacgc
     6421 tatcgcagca gttatttcgc ccatattttt ggtggcggat atgctgcagg ttattacgct
     6481 tatctgtgga cgcaaatgtt ggccgatgat ggttaccagt ggtttgttga gcagggcgga
     6541 ttaacgcgtg aaaatgggca gcgttttcgc gaggcgatcc tttccagagg taacagcgaa
     6601 gatctggaac gcctgcatcg acaatggcgt ggtaaggctc ctcagattat gccgatgctg
     6661 caacatcgtg gcttgaacat ataaacacca ccaatggctg gcctggagat atcgccaggc
     6721 cagcctggtt atagcagtaa ttgcagcagc tgggcgaggg caaacctcac cgctaaagct
     6781 aataccgttt cgcagtcgcc agcaaaatgc ataaccgcag tgtagttctg gcctttaata
     6841 tgccacgcga accagacggt acccgctggc gtaccatctt caccgccctc cggtccgccg
     6901 tagccggtaa tggcaatact gacatcagca tccgcacgct ctatggcacc tgttgccatt
     6961 tctgctgcca ctttctcact caccgcagaa tatcgttcaa gagactgctg gcttacgctg
     7021 aggattttca tctttgcctg atcggtgaaa gtaacaaagc ctgcaccgta aaatttgggt
     7081 gtatcttcag ctgcacacag ggcgctagcc aacttaccgc cggtgcatga ttcggcggta
     7141 gttaagcgta attgatccgc gataagtctt tgactcaacg cggatgtcag gttttcaata
     7201 gtgtcggtat ctgcgagttg tacaatttta tctttattaa tgttcactgt tttttgtcca
     7261 cgctattata tgtaaaggtg atttgctttt tctgtatgct cgtccaatat aataattttc
     7321 aaacatataa atccgattat tctgaaaaca tgtttaagac tcttcaatct tcttaacttt
     7381 agtgagtcaa attctctgaa tatttgacag ctagtgggct gattttaaat gccttataac
     7441 aattggcagt attcttattt ggcaattata aaaatagcaa ccagacttaa taagtcaaac
     7501 caaacgcaat taaggagtta gtatgaaatt tcagtccatt gtattagcaa gttttcttgt
     7561 catgccttat gcgttggcag atgaccaggg cggtttaaaa caggatgcag cgccaccgcc
     7621 gcctcatgca atagaagatg gctatcgcgg aaccgatgat gcaaaaaaaa tgaccgttga
     7681 tttcgcaaaa aacatgcacg atggcgcttc ggtttcacta cgcggtaatt tgatttctca
     7741 caaagg
//
LOCUS       DAZPCS010000165         7660 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZPCS010000165 DAZPCS010000000
VERSION     DAZPCS010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7660
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.167"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..816)
                     /gene="wcaD"
                     /locus_tag="ACHUQ7_003416"
     CDS             complement(<1..816)
                     /gene="wcaD"
                     /locus_tag="ACHUQ7_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="essential for colanic acid biosynthesis; colanic
                     acid is an exopolysaccharide produced under stress
                     conditions that confers acid and heat tolerance; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HFR1136682.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISIKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDALILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFRVGVVIAFPYISTRLGDLG"
     gene            complement(791..2008)
                     /gene="wcaC"
                     /locus_tag="ACHUQ7_003417"
     CDS             complement(791..2008)
                     /gene="wcaC"
                     /locus_tag="ACHUQ7_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFR1136683.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLNSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(2005..2493)
                     /gene="wcaB"
                     /locus_tag="ACHUQ7_003418"
     CDS             complement(2005..2493)
                     /gene="wcaB"
                     /locus_tag="ACHUQ7_003418"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFR1136684.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(2496..3335)
                     /gene="wcaA"
                     /locus_tag="ACHUQ7_003419"
                     /pseudo
     CDS             complement(2496..3335)
                     /gene="wcaA"
                     /locus_tag="ACHUQ7_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
     gene            complement(3428..5590)
                     /gene="wzc"
                     /locus_tag="ACHUQ7_003420"
     CDS             complement(3428..5590)
                     /gene="wzc"
                     /locus_tag="ACHUQ7_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFR1136685.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITVVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKNQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIIDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNCIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(5593..6036)
                     /gene="wzb"
                     /locus_tag="ACHUQ7_003421"
     CDS             complement(5593..6036)
                     /gene="wzb"
                     /locus_tag="ACHUQ7_003421"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFR1136686.1"
                     /translation="MFNNILVVCVGNICRSPMAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMDKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYHKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(6042..7181)
                     /gene="wza"
                     /locus_tag="ACHUQ7_003422"
     CDS             complement(6042..7181)
                     /gene="wza"
                     /locus_tag="ACHUQ7_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFR1136687.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALANAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            7490..7639
                     /locus_tag="ACHUQ7_003423"
     CDS             7490..7639
                     /locus_tag="ACHUQ7_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1136688.1"
                     /translation="MVIEYWLANNAGGSVTANGIAGKVTIRQLAIFKNYIKYHSIWFL
                     GVSLG"
BASE COUNT         1786 a         2008 c         1926 g         1940 t
ORIGIN      
        1 acctaaatcg cccagacggg tggaaatata aggaaacgcg attaccacac caacccggaa
       61 tacagccaga gaaatcaatg ctaacggtaa ttttttctta atcgcctctt tattcagata
      121 ttgaaatgcc cactccagca aataaaacag gataaaggtc ataacccctg aaaacgatcc
      181 ggataatatt atccctgcga gaatcagggc atctgtttta ggcgttttga taccaaactg
      241 tttgatgctg agccaaattg agattaatgc cagagcgaaa aatgccggtt cgaaataaag
      301 tgctgtcgtg cgcttgccgc cgaatttaat gaaattcagc acatagctgt tgctgtaaat
      361 cagatatttc gaaatcgact ccataatact gctgccaccg gtgaggataa tttgcgccat
      421 ctccaccgcc gccagcgcca ccaccagtcc gaccaccaga taaaagaaac gtaatatctt
      481 acgatggttg tgtggcgaaa ttgttttaaa acgaatactc cacaccatgc caataatgat
      541 cacaatatag acaaacagca tagttgaagt gacgtattta ccggcatcca acgactgacc
      601 aaacaggtag ttaaacgccg tgagtcccgc gccaatccct aaggcaatca tcaatttttt
      661 aatgctgatg cgttctaaaa acagcagtaa caagacaggt aaaaaagtga cgatggtgat
      721 ggggaaactt tcgccaagct gggcgatttt gacgttaacc agcaggtaga tcagtggcag
      781 cagcaggtag ctacagattc tgatagaagt tgacatactc ctccagcatc tgttgtccac
      841 tgtaggcggc gcgactgcgt tggctgaact cagccagcgt ggtaccaaat atcgcctgcg
      901 cgatttccgg tttgcttaac tgtaccagtt gcagcacctc ttcttcactg acggttttac
      961 caccggattt ttgcagcact tcccgcgccg catcgctatg ggtggcaatc accggcacgc
     1021 caatcgatag cgcctcacac aaaatcagcg ggtagttatc gacacgagaa ctgaacacca
     1081 gcgcatccat ctgattgagc gcgctcatca acttgcgctt gtcagtttca aagccgtgat
     1141 taaccacgtt gccagcggtg aacggcgaga acttaccaaa ggtatgcagt tcgattttgt
     1201 cgcccagcgc catcatctca cgcaccagtt gctggttagt tttgccgtcg taacgcaggt
     1261 catgcgccac cactgcgatt ttcggcttgc cctgagtttc gcgcaccgga ggcagatctg
     1321 ccagaatcgc ttcggttgcc atatcaatgc cattattgat aatccggcaa cgccctggcc
     1381 cgtacagact attgaaagcg tcagccacat gctggctggg ggaaataaac tgacagccca
     1441 gcgccagcat ctcgcggaat aactggcgtt tgcccgccac cagctggtgc gcgcgatcaa
     1501 tcttcaccgg cggatagtta tttaaggtcg ggcatttctg gcagcccgtt ttccagcctt
     1561 cgcaaccgtc ggtaaaggcg cagcgtccgg ttacactcca gtggtcgtgc agcgtccaga
     1621 ccagggtgac atccggttta tggtttttca ctttttcgca aaagcgcacc acgctgttaa
     1681 gattcaacca gtagctgtgc agcacatgaa aatgcaggac caccggaccc ggtgtgcgag
     1741 taatggtgcg atataactca ttgaaattgc cgaacagatc gcgattaaac agacgaaaca
     1801 acgcaatgtt cgccatcgcg gtcatccgcg gcgtatgttt gatgacctgc ggatagttct
     1861 gatggctgac gctctctttg ccgcctttgc cgtagccgta gacaaaatgt gacgccagcc
     1921 cctgttgcag cgcacgctgg tggagatcta acgccacacc tgctgccccg ccttccgcca
     1981 gtcgcacatt aaattgcaga atattcattt aatcaccttc actcgcgctt tttctcccac
     2041 caccagcgcg ttgtccggga cagagtcgag caccacgctg cccgcaccca cggtgacgtt
     2101 gttaccaagc gtgatatcac caagaataat gacgttggca ccgagttcga cgccgttgcc
     2161 aatgtgtgga catgccatgt tatcggcacc ccgattgccg atagtgacgc cgtggcgaat
     2221 ggtgaaatca tcccccgcca ccacgttttt attgatcact acggcgtaac cgtgatggat
     2281 agtaaagcgg cggccaatgg tcgcggcggc ctggatttca taaccgaaaa agcattcggt
     2341 gataatgcga tacagcacca gcagcggggc cgcccacaga ttgttgagga cgttcttttt
     2401 gcgccacacc gaacaaaaat gagcgacgcg ataggcaagt accatgcagc acgggcgtaa
     2461 actccagctg ttggcgcgca gatcttccag catctttagc gcccccgaat accatcagcc
     2521 agacgtttgc cgttacgcac cgacagcagc gtcaacaagg tgcgccaggt catacgttta
     2581 ttgcggatct ggtagagggt aaacagctga tattttttac tggcgcggtc gaatttgtct
     2641 ttgtgcttgc ggtaaaagtg gaagtatccg gagaattttt tcggtgacga ggtgatctgc
     2701 atttcgccgt gattgatatg caggatctgc gtcgcctctt ccactttcca cggttcgccg
     2761 tactccacca ccatccgcag gaatatgtcg taatcctgtg ctgctttcag ttcggtatcg
     2821 aacaaacact ctttgaaacg ccatgcccag gtaaagacct gattgccaat gatattacgt
     2881 ttatagaaca ggcggcgtga gtacggcgat ttgggataca gtggtaagct cgccggttgg
     2941 gaataaactt ctccctagca aacgtagtcg ttagcgtaca aaaaggcgtg tgtgaccagt
     3001 tgctgtttat gggcgaggaa gacgctcagg cggttgggcg tccattcatc atcatcatcg
     3061 atcccggtga tgtattcccc ttgcgccagc ataatcgcct ggttacgcac cgcgcacgcc
     3121 ccgctgttaa tgtcgttgtg gatgtaagtg atacgcggat cgttgagggc agtgacgtac
     3181 tgttgcagtt gttcccagga ggtggagcaa tcatccacga tgatcatctc ccagttgctg
     3241 tagtcctggc gcaggaccga ttttatcgcc cgaatcgcca gttgttggcg gttccaggtt
     3301 ggcatataga ttgagatcag cggattgtct ttcataggtt gttctcccgg cttatgagct
     3361 ggtttgcttg ccggatgcgg cgtgagcgcc ttatccggcc tacggagcgg tgcgaatgca
     3421 ggcgtcgtta tttcgcatcc gacttatatt cgtattcgta atagccataa tcctgatacg
     3481 cgctggcgcg gcggaagatg gagttcagaa tcaccccttt caccggaata cagttttgct
     3541 caaagcggct cagactggtt tccacttctt tcaatgtgtt gaccgcataa cgcgccacca
     3601 ttaacgtggt tccgacatga cgaccaacaa ttgccgcatc ggtcactgcc agaatcggcg
     3661 gcgtatcaat caacaccagg tcatagtttt tactcgccca gttcaccagt tcggcaaagc
     3721 gttcgctcat taacagttca gaaggatttg gcggcacctg accgcgcggg atcaggtcaa
     3781 atttggcaat agaggtcggt ttagcagcgg tagtaatatc gccctgacca atcagaatct
     3841 ccgacagccc attgacatta ttggtgccca acagctcgtg ggtgtagcct ttgcgcatat
     3901 cgcagtcgat caacaacacg cgtttattgg tctggctgat caccgccgcc aggttggcgc
     3961 agacaaaggt tttaccgatt gacgggctaa ccccggtcat catcaacaca ttgttctgcg
     4021 cctgcatcat cgcgaagtgc agactggtac gcagactacg gatggcttca atcgccagat
     4081 cggttggatt ccccactgcc agtagctggc tctgtttata gcgtttaacc cctttgatgg
     4141 ttttgacgct atcgcgcgct ttctgccact ccgacagcgg gatgctggca tagacgctga
     4201 ttccgtgttc ttccagcact tgcgggcttt cgatgccgcg attaaacaac gagcgcagca
     4261 gcacacccac gatagagagc atcaggccaa ggataatcgc gccgaggata atcagccctt
     4321 tcttcggttt tagcacgcca ggctgggtga ttgccgggtc aataatgcgc acatcgccga
     4381 cggtgctggc ttcggtgatt ttcagctcct gctctttatt cagcagttgc atatagacct
     4441 gctgaccaga ctcgacatcg cgggtcagac ggacaatttc ctgctggttt ttcggcatcg
     4501 ccgttacgcg accgttaagt ttggctttct cttcttccag cgcctgacgt ttctccagca
     4561 gcgtgcggta cgccgggtga actttggtgt acagcttgga gatttccgcc tctttaaagg
     4621 tcagttcgtt caactgcgcg tcgatgttca ccatcgaatc gagcaccgct ttcgcttcaa
     4681 gcggcagatc aacagaatct ttatcctgac ggaaggcatt cagtttgttt tcggcaacat
     4741 cgaggcggct acgtacttcc ggtaactgtt gcgcgaggaa agcgaggctt ttcgacgctt
     4801 ccgccgattt gcgctcaata ttttgttcct gatagttacg agcgatgctg ttaagaatgt
     4861 cgcggatctg ttcgcgatct tcaccggtat aagtcaggct cagtacgcct gcgtctttgc
     4921 cgttctccgt taccgtcagg ctgttttgca gttggttgat catccccaac gtggagtatt
     4981 tggtgacggt aaactcactg cccgggcggg cgtgaatggc ttcaaccatc agcgtgacgc
     5041 cttctttttt cagcatctgg cccgcttgcc cacgggcgct aaagccgcca tcactgctca
     5101 gggtgtagtt tttgtcatcc agcacattaa gcgtaaacac ctgatccgcc atctctttcg
     5161 ggcggttaaa ggtagtcact ttcaccgtct cgttctgacg ccccatcagg cgatcccagc
     5221 ccgccccgaa aatcgggaac gtgtttttgc tcaccgcaat atcgaggtcg agatcgtcca
     5281 ccgttttacc aagcaccaga cgcgaacgaa tcaactggat ctcggcgtcc gatgcaggcg
     5341 gtttgttggc taatgccgag ccgatatcct gcaccagcga attgccgctg ttttgctcga
     5401 tttgtaccag tgcgtcggca ctataaatcg gcgtggcgaa gaaagtgtaa accacggcac
     5461 agagggcaaa tacgacggtg atgccaatca cccaccagcg cgcttcaatg acggtgccga
     5521 ccaggcgacc aatatcgatt tcatcactgc ccgttaccgg agcggcatgt tgttttactt
     5581 tttctgtcat tcttatacct gctctgcgtt caatgcctgc gcccactggc gggcagaccg
     5641 ttcaagtaat gtgtacaccg ctgcaaacgt ttcccggctt ttgtgatacg gatcggggat
     5701 ttcacattcg ttatcccagt gaccaaacag catcactttg ccgcgcatct ccggtgccat
     5761 ctcgcataag cgttcgatat ggcgcttatc catggtcaaa atcaggtcat agttgcgaca
     5821 caggcggcgg ctgatttgtc gtgcacagtg gccttccaga gacagttgat gttctgcggc
     5881 gacgctgata gcggtaggat cagcgccctt accgaccagc gcgccgagtc cagcggactc
     5941 cactttcagt tccgggtgat aacgttgcag taagcgttcc gccatcgggg aacggcaaat
     6001 attgccgaca cagacaacta agatgttgtt aaacatgacg attaccagtt atgaatgtcg
     6061 ctggctgtat ccgtcatgta acggacaccg ctaatagttg gcagcaactg attgatcaga
     6121 cggttccagc gggaaaccgg ggcggtggtg acatacacca cgtcataagg ttgcaggcgg
     6181 aattctgtcg ccatcaccag cgacgtcgca tcggacatat ccagctggta gatattggca
     6241 atcttgccgt tacgcccgcc ctcgcctttc agcggacgaa tgacaaagat gccgctggcg
     6301 ttggaggtgg tcatgtcgat accttcggca ttggccagag cttcagtcag ggtcatgccg
     6361 ctaaagtcca ttttcagggt gctctgtttc ttcacttcac ccatcacaaa tactttcaga
     6421 tcatcattac gcggcacgta gagaatatcg ccgggataaa gcagtcggtt ctgattgagg
     6481 tcgccgtttt gcatcagcgc ctgtaaagaa atgcgttctt cacgaccatt gtgtgttagc
     6541 accacgttgc gccagtcagc ggtgtcggtc aggccacctg cggcgttgat ggcgtcgaga
     6601 atggtcagtg gtacgttggt gatcgcctgt tgaccggatt tattcacctg acctgagata
     6661 taggcctttt gtgagcggaa ggcggcgata ttaacgtcca cctgcgggtc agcgatgtac
     6721 gtcgctaagc gcccggtaat atcactgcgg atttcagcga gcgttttccc gactacgtga
     6781 accttgccga tatacgggta aaacatagtg ccgtcaggct gtacccagtt gccggtgtcg
     6841 ctggagctgc ggtactgacc agctggcgtg gtgagttccg ggtgatccca gacggtgaca
     6901 ttaagaacgt cccccggccc gacgcgatac tggtaattcg cgatctcgct ttccagcgtc
     6961 atattggggc gcgctacatt cgggcgtggg cgtaattggt caatcaggcg cggggtcagc
     7021 ggataaacat tcaccatttt gtcgagatcg aaatcagcgt cctgctgttt gatgacgtct
     7081 ttgcccatcg tcgacatatt gctgcccgga agtactgtgc aaccgcttat caaggttact
     7141 gacaccaata atggcatcaa tttcattttg gatttcatca ttgtttattt atcactttgg
     7201 cagagtaatt atcctgtgca ctattaatag caatgtcgcc atgcacattt accttgcagt
     7261 taattgaata aaaatttaac tggcatcagt cctaaaaaaa ttgatttcat ccgcaggcta
     7321 ttgacagaat aattcagact ggtctttcag gcatccagac acgctaccgc ccctggcttt
     7381 ttagctacca atacactgat tgagcttaat ttttcaaacc ctctctgcat acaatcgttg
     7441 atgagaaagg gttattgcgg aaattaactt ccgaatataa ggtgacatta tggtaattga
     7501 atattggctc gccaataatg caggaggaag tgttacagct aacggaatag caggcaaggt
     7561 aacaattcgg caattggcta tttttaagaa ttatattaag tatcattcaa tatggttttt
     7621 aggagtttct ttaggttgac aatatttaat attgcgtctc
//
LOCUS       DAZPCS010000166         7604 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZPCS010000166 DAZPCS010000000
VERSION     DAZPCS010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7604
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.168"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(127..1598)
                     /gene="yeeO"
                     /locus_tag="ACHUQ7_003424"
                     /pseudo
     CDS             complement(127..1598)
                     /gene="yeeO"
                     /locus_tag="ACHUQ7_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            1759..1834
                     /locus_tag="ACHUQ7_003425"
     tRNA            1759..1834
                     /locus_tag="ACHUQ7_003425"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1792..1794,aa:Asn,seq:gtt)
     gene            complement(1872..2822)
                     /gene="cbl"
                     /locus_tag="ACHUQ7_003426"
     CDS             complement(1872..2822)
                     /gene="cbl"
                     /locus_tag="ACHUQ7_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFR1136689.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEF
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFTRKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(2924..3841)
                     /gene="nac"
                     /locus_tag="ACHUQ7_003427"
     CDS             complement(2924..3841)
                     /gene="nac"
                     /locus_tag="ACHUQ7_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFR1136690.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            4169..4244
                     /locus_tag="ACHUQ7_003428"
     tRNA            4169..4244
                     /locus_tag="ACHUQ7_003428"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4202..4204,aa:Asn,seq:gtt)
     gene            complement(4300..5235)
                     /gene="ldtA"
                     /locus_tag="ACHUQ7_003429"
     CDS             complement(4300..5235)
                     /gene="ldtA"
                     /locus_tag="ACHUQ7_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR1136691.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNIRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(5297..6376)
                     /gene="cobT"
                     /locus_tag="ACHUQ7_003430"
     CDS             complement(5297..6376)
                     /gene="cobT"
                     /locus_tag="ACHUQ7_003430"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFR1136692.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLFKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(6388..7131)
                     /gene="cobS"
                     /locus_tag="ACHUQ7_003431"
     CDS             complement(6388..7131)
                     /gene="cobS"
                     /locus_tag="ACHUQ7_003431"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFR1136693.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLALMTGEFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLLGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(7128..>7604)
                     /gene="cobU"
                     /locus_tag="ACHUQ7_003432"
     CDS             complement(7128..>7604)
                     /gene="cobU"
                     /locus_tag="ACHUQ7_003432"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFR1136694.1"
                     /translation="SSQVLYIATSQILDDEMAARIEHHRQSRPEHWRTVERWQHLDEL
                     IHADINPNEAVLLECVTTMVTNLLFDYGGDKDPDEWDYQAMEQAINAEIQSLIAACQR
                     CPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQQLAAAANEVWLVVSGIGVKIK"
BASE COUNT         2099 a         2057 c         1649 g         1799 t
ORIGIN      
        1 cgttctaccg actgaactac agaggaatcg tgagaacgag gcgaatatta gcgatgccca
       61 cccacaatgt caaagcctgt tttttaaatt tgaaatcgtt tgctgaaata atctgcattt
      121 tgtcgtttat tccgacacaa ctggcttttt ttcacacttt tgcggctcgg atcgagggta
      181 tttccacagc caacgtccag taaccattcg ccagtaaaac agcacggccc gcacagccca
      241 gtcggcaaac attcccatcc agacaccaac cacaccccag ccaagcatga ttccctgcac
      301 ataaccgact acaacccggc aaccccacat gctcaacata gaaacccaca tggcgtaacg
      361 ggcatcacga gcacccttaa atccagcggg tagcacccat gaggcggacc aaataggcat
      421 aaataaagca tttagctaaa tcagaatcac aacgacgtgt ttaacctgtg gatcctgggt
      481 gtaaaacgat gccataaccc cggcaaaggg agccgttagc caggcgatgg ccgttaatcc
      541 aagagtggac agccagaaca catgccgcaa ctgaatctct gcttgcgcta tctgccctac
      601 ccccaacctt cggcctgtaa tgatcgtaga agcagaaccg agcgcacttc cgggtaagtt
      661 gataagagcc gcaattgaga acgcgataaa atttccggca ataacgctgg tccccatccc
      721 ggcaacgaac atttgggtta ataaccgacc actggtaaat aacactgatt cgacactcgc
      781 tggaatacca atccccatga cttcccagat aatgctaaaa ttcagcggtt taaaatagct
      841 ctttaacgaa atccttagcg caggattaaa accaatcgcc agcacccaca aaattgcaac
      901 tgcgccaata taacgagaaa tggttaaacc caacccagcc ccgaccagga gaaaaggccg
      961 taaatcaata tgccgctaat aataatatta agaatattca ggctaccgtt aatcagtagc
     1021 ggtattttcg tattccctgc accacgaagt gccccgctac caataagagt gatggcagcg
     1081 gctggataac tgagtacggt cagctccaga taagtcaacg ccagtgcttt aacttctgtc
     1141 gtggcatcac ccgcgacgaa atcaataatt tgttcgccaa aatgatgaat tagcgttgcc
     1201 aacagtacgg caaacaacgt catgatcacc aatgactgcc gcgtcgccac cctcgctcgt
     1261 cgtcgatccc gcttaccgag actaaatgct acaacgacag tagtaccaag atcgatagct
     1321 gcaaaaaaag ccataatgac catattgaag ctgtccgcca accccacgcc ggccatcgca
     1381 tcttttccca gccaactgac cagaaaagtg ctcaggaccc ccatcaacag gacacaggcg
     1441 ttctccatga agataggaac agcaagcggg gttatctcgc gctagaacaa cactttgtag
     1501 ctcttgcgtt tagagtgcca gcgagtgccg tgaacaacct ggcgtaaagc agaggagata
     1561 ttcaaagccg accttaattg cagaaagtga aaccacattt caaatgatga gggagaatca
     1621 gcaaagctgc aaagattttc gccaacaaat tgtctgcaaa tgcaacaaac tgttgataga
     1681 aacggcaaac agttgggaaa tttaaaaatc gggtttgaca aaatattttt cgccgttaag
     1741 atgtgcctca acagcgattc ctctgtagtt cagtcggtag aacggcggac tgttaatccg
     1801 tatgtcactg gttcgagtcc agtcagagga gccaaatttc tgaaaagccc gcttttatag
     1861 cgggcttttt gctatatctg ataatcaatt tcctcttcac tgttttccat cacctggcgc
     1921 ttgatatcct caactgacag tcctgcatta caaagttcca gaaagcgcca aacatagtta
     1981 cgctgaagtt gtcctcgctt cagtcccaac cagacagtat tagcatcgaa aagatgccgc
     2041 gtatccaggc ggattaaatt ctcttcctct tgttcgccgc tggattgttc ggcaactaat
     2101 ccgatcccaa gcccaagagc aacataggtt ttaatgacat cagaatcctg tgcacttaat
     2161 acaatatctg ccggcaaacc tttgcgggta aatgcgtcat cagtgcgtga gcgccccgta
     2221 attccctgtc ggtaagtaat taacggccac ttcgctattg attccagcgt caatggtgta
     2281 atttgcgtta agggatgatc gagtggaaca agcaaactat ggtgccaaca aaaccatggg
     2341 aaggcgacga gctgcgggtc attactcaaa cgctcgctgg cgataccaat atcagcttcg
     2401 ccattttgca acaacgtcgc aatttcctgt ggcgaaccct ggattagctc gagccgaacc
     2461 tccgggaaaa gttcgcgaaa agctttaatg acctctggca agctataacg tgcctgagta
     2521 tgcgtcgttg caatagtgag aacaccagac gtatcgttgg taaacaggtc tgcaagccga
     2581 cgaacattac tggcttcatt cagaatacgt tctgcaatga ccagtaatgc tttgcccggt
     2641 tcagtcatcc ccagcagtcg cttacctcgt cgaacaaata tttcgatgcc aaattcatcc
     2701 tccagttccc gaatatgacg gctaacgcct gactgtgagg taaaaagcat attcgcaacc
     2761 tctgtcaggt tgtaatcctg acgtgcagcc tcgcggatta tctttagttg ttggaaattc
     2821 acggtaaact ccgggcagtt cagatatccc gttattgtta aagtctaatg cccggcataa
     2881 caaataataa aaacccgcat cttattccat cccgatataa cgcttagctc accaattgcc
     2941 actgcctttt ttccatcact ggagaactaa tcactgacat taacaactct ttcactgcct
     3001 gtgcctgtgg tgacaagttt gctctggcgg gtaaatttaa ggacaaagag agactcattg
     3061 aaggagtggt aatgcgtgac atccacccat ttactgcgcc acataacgaa cgcgcagccg
     3121 attcaggtaa tactgcaacg cccatgccgc tggcaatcgc tgcggtaagc gtggcaatag
     3181 actcaatttc accaatgact tttgccgtga gccgccgtag ggaaaaagcc tcatcaacac
     3241 gaagtctaac agcgctgtaa tcactgggga gaaaaaggtt catttgcgca atagcattaa
     3301 catcaacgtt ttgccccggg caatcttgag ttcctaccag gaaaagatct tctttcagca
     3361 aagcctgact ggatacccca gccacagggg aatgctcata aatcaccgcc atatcgagtt
     3421 ggtgatttat cagtttttcg ttaagcactg caccactatt ttcatgaaga tagataacga
     3481 cctccgggaa ttcagcgcga accgcctgta ataagggcat ggtgatggat gacgcagcgg
     3541 ttcctggcgc aaagccaatc gagacttgcc ccgataatga ctgaccaacg ttatgcaccg
     3601 ccagttgggc ctgctcacac tgacgtaaaa tggcccgtgc atgggtatag agaatttttc
     3661 cggcgtctgt tggcgtaacg ccccgttttg tgcgaatcaa aagctgttga tttaactcac
     3721 cttccagtat ggcaacctgt tggctgagcg ctggttgtgc gatatgcaat acttcagcag
     3781 cctgggtcag gctaccaata tctacaattt ttacgaagta tttcaggcgt ctgaagttca
     3841 tgttgcctcc ggtttttaag aatcggccca agtgccgcca ttacttacaa ccagtatgca
     3901 agatgcctgc cagttctatt ttggcgttga tgcgcaagct aaccaactgt caaataagag
     3961 attatgatag attcgtcatt ggcccttttt atcagcagtc gcattcacct gccctataaa
     4021 aggagggtat gcaccacgat ggttcattac ccaataagat tgaaagctca ccactttgtt
     4081 gaaattggca gcaaacaaac aaaaaaaatg catttcaccc tttgacatca ccatgcactg
     4141 ccattaatat gcgccccgtt cacacgattc ctctgtagtt cagtcggtag aacggcggac
     4201 tgttaatccg tatgtcactg gttcgagtcc agtcagagga gccaaattca aaaaagcctg
     4261 ctttcgagca ggctttttac tttctaatta ccaacgctct taaaacatct gtcttgaacc
     4321 agaactaatt tgcacaggca ttcccgatcg atgttgcaac gcagcatttg cgcgatttac
     4381 atcaacttct tgcccgttga taaacgcccg caaagatggg gttaccggca atggcacttt
     4441 tcggtcagac tcatattctg cacgattgcg cgacagcggc tcatgaactt ccagccactt
     4501 tgaaccatcc ggttccgttg tgtatttcac tggctgatca ataatttgta cacgagtgcc
     4561 tacaggaaca ttatcaaaca gatatttgat atcgtcatta cgcagacgaa tacagccctg
     4621 acttacacgg agcccaatac caaaattggc attggtaccg tggatggcat acaacctgcc
     4681 aatataaatc gcgtacagcc ccatggggtt atcgggcccc gcaggaacaa atgcgggcaa
     4741 actctcccct cgtttcgcat attcgcgccg gatgttcggc gttggcgtcc aggttggtgc
     4801 ttcttgttta cgttcaacgg tagtcaccca gttacgcggg gtttctcgcc cagcctggcc
     4861 gataccaata ggaaagactt ccactgtatt actgtctggc ggatagtaat aaagacgcat
     4921 ttcagcgacg ttaaccacaa tccctttacg aacagtggcg ggcaaaatca gttgctgcgg
     4981 aatggtgagt tgtgagccag gcttcggcaa aaaaacatca gcgcccggat tcgcttccag
     5041 catattactt aacccttgcc cgtattgtgc ggcaaaagtc tccagcggct gagtattgtg
     5101 atcaggaaca gttacagtaa gcgactgccc cactaaacgg ctaccctctg gaggtaatgg
     5161 ataagttacc gccaggctat tttggctggc aaaaagcaga gcaaatgagc aaagaatatt
     5221 tacacgacgc atcatgtccc tttcctaagt cgcgaatact atccgataag tatagctttt
     5281 atcagacttt tcgtttttag ctgttcaaat cagaagtcgt attccccggt agaacaatat
     5341 tactggcagc aagttcgccc atgttgttgt atatcgcaca agcagcttcg atgatggaca
     5401 tcgccagagc tgcaccactc ccctcaccta aacgcatatc catattgaga taaggctcca
     5461 gccccaaatg cgagagcgct atacgcgcgc ctttttctgc cgacaagtga gaaggaatga
     5521 gatacggttt gattgcagga gacatctggc aggctgcgag cgcagcagca taagaaagaa
     5581 atccatccag caacacgggt aaaccgcagg aagcagcacc taacatcact ccagctattc
     5641 cgaccaaatc aaatccaccc acttttgcca ggacatcaac accatcctga ggatttggtt
     5701 gattcaacgt aatcgcccga cgcacaacat caattttatt agccagttta tctgtcggca
     5761 ggtttgcgcc aatcccaacc acttcttcag gatcccggcc agtgattgtg ctgactattg
     5821 ccgctgccgg tgtcgtgttt gccatcccca gttcacctac accaaacagc gtgacaccgt
     5881 ttttcgccag ctcccgcgta taacatatga cgtccaaaag caacttttca gcctgacggc
     5941 gactcattgc cggagctgaa gcaatattgc cgctacctcg tgcgacacgc atgttgataa
     6001 gcccggggat aggctcagca gtatcaatac caacatcaat tacgtggacg ttagcgcccg
     6061 cttgtgctgc cagcacacac acgccggttg ttccacgggt catattttca gcctgtatgg
     6121 ctgtcacttc ttttggagaa atagcgaccc cttcctccca gacgccgtga tcggcacaca
     6181 taaccagtac cgcttttttg cccacatgcg gtatgccatt cacccccggc attcctgcca
     6241 gttgtatggc aagcgcctcc agctttccta ggctaccaac aggtttgaat aacccgtcaa
     6301 catgccgttg tgcacgcgac atagcggcag aattgatggc tgggatcgta ttcagtaaat
     6361 cggcaattat ttgcatgtca cgtttcttta tagcagtgcc agcaggaaga ccagttcacc
     6421 aagctctatg gctgctccca gcgtatcgcc cgtttgcccg cccagcgtac gtttgagaag
     6481 ctgaccgagg atgaaaatcg ccaccatcgt caccaccata gcagccacac catgcatacc
     6541 cagcaataat acggcagcaa aaatagcggc taagccgagt gtgacgcagg tttgtcgccc
     6601 atcaattttg ccaatgaaca cattgccaag cccttcttcc cgcgcataac gatgacgata
     6661 catcaataat gcggcagtcc cgcggctgac cgcacatgct gccgccagcg atgcaaggat
     6721 cggttcgcca cgcagagcca actcgcttaa caccagaatc tttgccagta ccacaaaaat
     6781 caatgccaga ccgccgtggg tgcctaaacg gctatcacgc atgatttcca acatgcgatc
     6841 gcgactacgt gcagaaaata cgccatcaca ggtatcagcg aggccgtcca gatggaattc
     6901 cccggtcatc agcgccagca caagtacgct aaacagtgcc gccagtggtg cgccacacca
     6961 tgcctgcagc accatgaaga ccagcccgct aatcgcgcca agcaataatc caatcaaagg
     7021 aaaagtaata ataccgcgag aataatgttc gaaatccagt ccctgggacc agcgacgcgg
     7081 tacaggcaga cgcgtaataa atgagagcat cgcccaaaat aatttgctca tttgattttt
     7141 actccaatac ccgaaaccac cagccatact tcatttgccg cagcggccaa ctgctgattt
     7201 acccgcccgg caatatcacg aaaatgtcgt gccagacgac tctccggcac aatccccatt
     7261 cccacttcgt tagtcactaa tacaaccttt gcggggcaac gttggcaggc agcaatcaac
     7321 gactgaatct cagcattaat cgcctgttcc atcgcctgat aatcccattc atcagggtct
     7381 ttatcgccgc cataatcaaa caacagatta gtcaccattg tggtaacgca ttccagcaac
     7441 acagcctcat tcgggttaat gtctgcatga attaattcat caagatgttg ccagcgctcc
     7501 actgtgcgcc agtgctccgg gcggctttgc cgatgatgtt ctatccgtgc agccatctca
     7561 tcatcaagga tttgcgaggt agcgatatac agaacctgtg aaga
//
LOCUS       DAZPCS010000167         7536 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZPCS010000167 DAZPCS010000000
VERSION     DAZPCS010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7536
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.169"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            1..1160
                     /gene="rbsA"
                     /locus_tag="ACHUQ7_003433"
                     /pseudo
     CDS             1..1160
                     /gene="rbsA"
                     /locus_tag="ACHUQ7_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
     gene            1165..2130
                     /gene="rbsC"
                     /locus_tag="ACHUQ7_003434"
     CDS             1165..2130
                     /gene="rbsC"
                     /locus_tag="ACHUQ7_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFR1136695.1"
                     /translation="MITQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            2155..3045
                     /gene="rbsB"
                     /locus_tag="ACHUQ7_003435"
                     /pseudo
     CDS             2155..3045
                     /gene="rbsB"
                     /locus_tag="ACHUQ7_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            3171..4100
                     /gene="rbsK"
                     /locus_tag="ACHUQ7_003436"
     CDS             3171..4100
                     /gene="rbsK"
                     /locus_tag="ACHUQ7_003436"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFR1136696.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            4104..5096
                     /gene="rbsR"
                     /locus_tag="ACHUQ7_003437"
     CDS             4104..5096
                     /gene="rbsR"
                     /locus_tag="ACHUQ7_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFR1136697.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(5062..6489)
                     /gene="mdtD"
                     /locus_tag="ACHUQ7_003438"
     CDS             complement(5062..6489)
                     /gene="mdtD"
                     /locus_tag="ACHUQ7_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFR1136698.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGDAMMMPVARPALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(6512..7204)
                     /locus_tag="ACHUQ7_003439"
     CDS             complement(6512..7204)
                     /locus_tag="ACHUQ7_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709569.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFR1136699.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWITEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         1918 a         1825 c         1986 g         1807 t
ORIGIN      
        1 aaaaccatgt atgtcgaagc ggataaattg ctggctaaac ttaacctgcg ctttaaaagc
       61 gacaagttag tgggcgatct ttccatcggt gaccagcaaa tggttgaaat cgccaaagtg
      121 ctgagctttg agtcgaaagt catcattatg gatgaaccga ccgatgcgct gaccgatacc
      181 gaaaccgaat ccctgttccg cgtcatccgc gagctgaaat cgcaaggcca ctgtattgtc
      241 tatatctccc accgcatgaa agaaatcttc gagatttgcg atgacgttac cgtttttcgt
      301 gatgggcaat ttattgctga gcgcgaagtg gcatcactga ccgaagattc gctgattgag
      361 atgatggtgg gccgcaagct ggaagatcaa tatccacacc tggacaaagc gccaggagat
      421 atccgcctga aagtcgataa tctctgcgga cctggcgtta acgatgtctc ttttacttta
      481 cgcaaaggcg aaattcttgg cgtctctggt ttgatgggcg caggtcgtac cgaactgatg
      541 aaagtgctct acggcgcact gccgcgcacc agcggttacg tcaccctgga tgggcatgaa
      601 gtcgttaccc gttcaccgca ggatggcctg gcaaacggca ttgtgtatat ctccgaagac
      661 cgtaaacgtg acggtttagt gctgggcatg tcagtaaaag agaacatgtc gctgacagcg
      721 ctgcgctact tcagccgcgc tggcggcagt ttgaagcatg ccgatgaaca gcaggctgtg
      781 agtgatttca ttcgtctgtt taatgtgaaa acgccgtcga tggaacaggc aattggtctg
      841 ctttccggtg gcaatcagca aaaagtggcg attgcccgcg gtctgatgac acccccaaag
      901 tattgatcct tgatgagcct acccgtggcg tagatgtcgg cgcgaaaaaa gagatctatc
      961 aactgattaa ccagttcaaa gccgatggct tgagtatcat tctggtgtca tcggagatgc
     1021 cagaagtatt aggcatgagc gatcgcatca tcgtcatgca tgaagggcat ctcagcgggg
     1081 aatttactcg tgagcaggcc acccaggaag tgttaatggc tgtcgctgtg ggcaagctta
     1141 atcgcgtgaa tcaggagtaa aaaaatgata acccagactg tctctggtcg ccgttatttc
     1201 acgaaagcgt ggctgatgga gcagaaatcg cttatcgctc tgctggtgct gatcgcgatt
     1261 gtctcgacgt taagcccgaa ctttttcacc atcaataact tattcaatat tctccagcaa
     1321 acttcggtga acgccattat ggcggtcggg atgacgctgg tgatcctgac gtcgggcata
     1381 gacttgtcgg taggttctct gttggcgcta accggcgcag ttgctgcatc tatcgtcggc
     1441 attgaagtca atgcgctggt ggctgtcgct gctgctctca cgttaggtgc cgcaattggt
     1501 gcggtaaccg gggtgattgt agcgaaaggt cgcgtccagg cgtttatcgc tacgctggtt
     1561 atgatgcttt tactgcgcgg cgtgaccatg gtttatacca acggtagccc agtgaatacc
     1621 ggctttactg agaacgccga tctgtttggc tggtttggta ttggtcgtcc gctgggcgta
     1681 ccgacgccag tctggatcat ggggattgtc ttcctcgcgg cctggtacat gctgcatcac
     1741 acgcgtctgg ggcgttacat ctacgcgctg ggcggcaacg aagcggcaac gcgtctttct
     1801 ggtatcaacg tcaataaaat caaaatcatc gtctattctc tttgtggtct gctggcatcg
     1861 ctggccggga tcattgaagt ggcgcgtctc tcctctgcac aacccacggc ggggactggc
     1921 tatgagctgg atgctattgc tgcggtggtt ctgggcggta cgagtctggc gggcggaaaa
     1981 ggtcgcattg ttgggacgtt gatcggcgca ttaattcttg gcttccttaa taatggattg
     2041 aatttgttag gtgtttcctc ctattaccag atgatcgtca aagcggtggt gattttgctg
     2101 gcggtgctgg tagacaacaa aaagcagtaa taacgactac aggacatctt gaatatgaac
     2161 atgaaaaaac tggctaccct ggtttccgct gttgcgctaa gcgccaccgt cagtgcgaat
     2221 gcgatggcaa aagacaccat cgcgctggtg gtctccacgc ttaacaaccc gttctttgta
     2281 tcgctgaaag atggcgcgca gaaagaggcg gataaacttg gctataacct ggtggtgctg
     2341 gactcccaga acaacccggc gaaagagctg gcgaacgtgc aggacttaac cgttcgcggc
     2401 accaaaattc tgctgattaa cccgaccgac tccgacgcag tgggtaatgc tgtgaagatg
     2461 gctaactagg cgaacattcc ggttatcact cttgaccgcc aggcaacgaa aggtgaagtg
     2521 gtgagccaca ttgcatctga taacgtactg ggcggcaaaa tcgccggtga ttacatcgcg
     2581 aagaaagcgg gtgaaggtgc caaagttatc gagctgcaag gcattgccgg tacatccgca
     2641 gcccgtgaac gtggcgaagg cttccagctg gccgttgctg ctcacaagtt taatgttctt
     2701 gccagccagc cagcagattt tgatcgcacc aaaggtttga acgtaatgca gaacctgttg
     2761 accgctcatc cggatgttca ggctgtattc gcgcagaatg atgaaatggc gctgggcgcg
     2821 ctgcgcgcac tgcaaactgc cggtaaatcg gatgtgatgg tcgtcggatt tgacggtaca
     2881 ccggatggcg aaaaagcggt gaatgatggc aaactagcag cgactatcgc tcagctaccc
     2941 gatcagattg gcgcgaaagg cgttgaaacc gcagataaag tgctgaaagg cgagaaagtt
     3001 caggctaagt atccggttga tctgaaactg gttgttaagc agtagtttta atcaggttgt
     3061 atgacctgat ggtgacataa atacgtcatc gacagatgaa cgtgtaatat aaagaaaagc
     3121 agggcacgcg ccaccctaac acggtggcgc attttatgga catcccgaat atgcaaaacg
     3181 caggcagcct cgttgttctt ggcagcatta atgctgacca cattcttaat cttcaatctt
     3241 ttcctactcc aggcgaaacc gtaaccggta accactatca ggttgcattt ggcggcaaag
     3301 gcgcgaatca ggctgtggct gctgggcgta gcggtgcgaa tatcgcgttt attgcctgta
     3361 ctggcgatga cagcattggc gagagcgttc gccagcagct cgccactgat aacatcgata
     3421 ttactccggt cagcgtgatc aaaggcgaat caacaggtgt ggcgctgatt tttgttaatg
     3481 gcgaaggtga gaatgtcatc ggtattcatg ccggcgctaa tgctgctctt tccccatcgc
     3541 tggtggaagc gcaacgtgag cgtattgcca acgcgtcagc gttattaatg cagctggaat
     3601 caccactcga aagtgtgatg gcagcggcga aaatcgccca tcaaaataag actatcgttg
     3661 cgcttaaccc ggctccggct cgcgaacttc ctgacgaact gctggcgctg gtggacatta
     3721 ttacgccaaa cgaaacggaa gcagaaaagc tcaccggtat tcgtgttgaa aatgatgaag
     3781 atgcagcgaa ggcggcgcag gtactgcatg aaaagggtat ccgtactgta ctgattactt
     3841 taggaagtcg tggtgtatgg gctagcgtga atggtgaagg tcagcgcgtt cctggattcc
     3901 gggtgcaggc tgtcgatacc attgctgccg gagatacctt taacggcgcg ttaatcacgg
     3961 cgttgctgga agaaaaacca ttgccagagg cgattcggtt tgcccatgct gccgctgcga
     4021 ttgccgtaac acgtaaaggc gcacaacctt ccgtaccgtg gcgtgaagag atcgacgcat
     4081 ttttagacag gcagaggtga cgcttggcta caatgaaaga tgttgcccgt ttggcgggcg
     4141 tttctacctc aacagtttct cacgttatca ataaagatcg cttcgtcagt gaagcgatta
     4201 ccgccaaagt tgaagcggcg attaaagaac tcaattacgc gccatcagct ctggcgcgta
     4261 gcctcaaact caatcaaaca cataccattg gcatgttgat cactgccagt accaatcctt
     4321 tctattcaga actggtgcgt ggcgttgaac gcagctgctt cgaacgcggt tatagtctcg
     4381 tcctttgcaa taccgaaggc gatgaacagc ggatgaatcg caatctggaa acgctgatgc
     4441 aaaaacgcgt tgatggctta ctgttactgt gtaccgaaac gcatcagcct tcgcgtgaaa
     4501 tcatgcaacg ttatccgaca gtgcctaccg tgatgatgga ctgggctccg ttcgatggcg
     4561 acagcgatct tattcaggat aactcgttgc tgggcggaga cttagcaacg caatatctga
     4621 tcgataaagg tcatacccgt atcgcctgta ttaccggtcc gctggataaa actccggcgc
     4681 gcctgcggtt ggaaggttat cgggcggcga tgaaacgtgc gggtctcaac attcctgatg
     4741 gctatgaagt cactggtgat tttgaattta acggcgggtt tgacgccatg cgccaactgt
     4801 tatcacatcc gctgcgtcct caggccgtct ttaccggaaa tgacgctatg gctgttggcg
     4861 tttaccaggc gttatatcag gcagagttac aggttccgca ggatatcgcg gtgattggct
     4921 atgacgatat cgaactggca agctttatga cgccaccatt aaccactatc caccaaccga
     4981 aagatgaact gggggagctg gcgattgatg tactcatcca tcggataacc cagccgaccc
     5041 ttcagcaaca acgattacaa cttactccga ttctgatgga acgcggttcg gcatagattt
     5101 acgctgtctt ttgatcaaat tattaccatc ggttgttttc agaagcatga acattgctgc
     5161 tgaagcaaca gtaataatgc ccatcgtgat aaacgtatag tggaattgtt cgacagtcgt
     5221 tgtgccttcc atcccttcat aaatgcgaag gacggccgca cttacagcaa cgcctaaact
     5281 aatcgacagt tgctgcgtga ccgccagaac actgttaccg ctgctggcgt tgtcatcggt
     5341 cagatcggca agtgtgatgg tattcatcgc ggtaaactgc gtcgacatag ccatccctaa
     5401 tataaacaac ggcaagatca gcatccatat cgccattgcc ggtgattgca aagagaactg
     5461 agcgatcatt agcccaataa tcaccgtgat ccccactaat gtatggcgat agcccagacg
     5521 acgtaagact tgggtaacca tcgattttgc aataatggaa cctaacgctg tcggtgccat
     5581 catacagccg gcaataaacg cctgataacc aaatcctacc tgtaacatca atggcataag
     5641 gaacggtaca cagccggtcc ccagacgggt tgcaatattg cctacgatac cgatcgagaa
     5701 agtgcgggtt ttaaataaat ctaatgaaat taatgggttt ggcgtacgtc gcgcatggag
     5761 aatatagaga agcagtaacc cgatgctggt gacaattacc gtcaaggcaa tccagctggc
     5821 gacaatcttt tccccgaata gctctattcc gcttgaaaag agaacaaggc tgaggccaaa
     5881 cagcaaaaag ccagtgatat cgaatctgcg tcgtgcggtg gtgaaattgg gcatatgttt
     5941 gcgcgcgtaa agaaggcccg caatacctat ggggatatta attaaaaata tccagtgcca
     6001 ggttgcccag gtgaccagca cgccgccaag aacggggcct aaaattggcc ccaccagacc
     6061 cggcatggcg acaaaattca ataccggaag aagttcatta cgaggataag cacgcagtaa
     6121 ggccggccga gcaacaggca tcatcattgc gtcgcctatc ccttgaataa cccggaagac
     6181 aaccagctgt ggtagcgaat tagaaagtgc gcaggccaga gaacccaacg tgaacagact
     6241 cacggcaagg gtaaaaatgc gacgcgtacc gaagcgatcg gctagccatc cgcttaccgg
     6301 aataagcatc gccaccgtca gcgtataact gatgatggct gattgcatcg cgagaggaga
     6361 acgattaagg ctatgagcga ttgcgggtaa ggcggtattc agaatagtgg catcaagtgc
     6421 ctgcatgaag aaggccatcg ccgcgatcca cggcaaaccc gacatactac gcttcttttt
     6481 atcgctcatt caatgtcctg ttatcgggtt atcacttatc aggtgagcgt agcagcgcct
     6541 gacaagcttt aaatgccgcg tcgccatcgc tttggataat cgcatcgaca atcgcctggt
     6601 gcagatccag ctttatcact gtgtcgctgg taattgacgt gaagtaagtg tgataaaccg
     6661 aatggaatag cgaggcgaat gaggtcaaaa acggattggc gctcatttca tagatatgct
     6721 catgccaggc catatcgact tcgatccagc gttcacggcg aaagttctct tttaatgccg
     6781 ccatttcggc cattaacgta ttgagatgcg ccttctgttc cgcggtgcca accgttgctg
     6841 ccagtaggca ggcttgcggc tccagacaga tacgcataac cagaaagtga tcgatgacct
     6901 gatgaaagtt ctcttctgtt atccaccaag taagcaattc ctgatcaaga aaattccagt
     6961 tcgattgtgg catgacccga gtaccaattc gcggtcgcgg taaaaccatc ccttttgccg
     7021 ttaacgtttt gaccgcttcg cgtaccgctg tacgactcac tccaaattgc tcgcccagct
     7081 caatctcacc gggcaaaatg gtgccgggtt catattcacc ttttaagatc cgttgcgcca
     7141 gcttctcagc cagaacatac gaaaggtttt tctgtgcagc taactgttgt gcgcttaaag
     7201 gcattactta tcttcctttt tctttttatt cctccttagt atgccaccag gaagtgtgat
     7261 tacggttgca aaaacggcaa attgcttgtt ttatggcaca ttaacggggt gttttgctga
     7321 aaaaatgcgc ggtcagaaaa ttattttaaa tttcctcttg tcaggccggg ataactccct
     7381 ataatacgcc accactgaca cggaacaacg gcaaatacgc cgccgggtca gcggggttct
     7441 cctgagaatt ccgacagaaa aagcaaaaat aaatgattga ctctgtagcg ggaaagcgta
     7501 ttatgcacac ccccgcgccg ctgagaaaaa gcgaag
//
LOCUS       DAZPCS010000168         7532 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZPCS010000168 DAZPCS010000000
VERSION     DAZPCS010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7532
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.170"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            60..1112
                     /locus_tag="ACHUQ7_003440"
     CDS             60..1112
                     /locus_tag="ACHUQ7_003440"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HFR1136700.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            1188..2621
                     /locus_tag="ACHUQ7_003441"
     CDS             1188..2621
                     /locus_tag="ACHUQ7_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HFR1136701.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKLIAYLLIGKIGNTMLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            2804..4984
                     /locus_tag="ACHUQ7_003442"
     CDS             2804..4984
                     /locus_tag="ACHUQ7_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFR1136702.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVEPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(5125..6408)
                     /locus_tag="ACHUQ7_003443"
     CDS             complement(5125..6408)
                     /locus_tag="ACHUQ7_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFR1136703.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEEKK"
     gene            complement(6543..>7532)
                     /locus_tag="ACHUQ7_003444"
     CDS             complement(6543..>7532)
                     /locus_tag="ACHUQ7_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1136704.1"
                     /translation="LLKKRTNGSVRYCQYTSITEAIQANIELFSGHKHKPLTARINSD
                     NSVTLHSWLDRYEKILASRGIKQKTLINYMSKIKAIRRGLPDAPLEDITTKEIAAMLN
                     GYIDEGKAASAKLIRSTLSDAFREAIAEGHITTNPVAATRAAKSEVRRSRLTADEYLK
                     IYQAAESSPCWLRLAMELAVVTGQRVGDLCEMKWSDIVDGYLYVEQSKTGVKIAIPTT
                     LHVDALGISMKETLDKCKEILGGETIIASTRREPLSSGTVSRYFMRARKASGLSFEGD
                     PPTFHELRSLSARLYEKQISDKFAQHLLGHKSDTMASQYRDDRGREWDKIEIK"
BASE COUNT         1798 a         1781 c         1923 g         2030 t
ORIGIN      
        1 ctttgtcctt aatgtaacca tatcgcaata agttatgttt ttaaattgag ggcattatta
       61 tgaaaaaaat accctgcgtg atgatgcgag gtggaacctc gaggggcgcg ttcctgttag
      121 cggaacattt acccgaagat caaacgcagc gcgataaaat attgatggca attatgggtt
      181 ccggtaacga tctggaaatt gacggtattg gcggcggtaa tccactgacc agtaaagtcg
      241 ccattattag ccgttccagc gatccgcgtg ctgatgtcga ttatctgttt gctcaagtta
      301 tcgtccatga gcaacgtgtc gataccacgc ctaactgcgg caatatgctg tctggcgtag
      361 gggcattcgc cattgaaaat ggtttgattg cagcgacttc gccagttacc cgtgtacgta
      421 tccgcaacgt caatacgggt acgttcatcg aagctgatgt gcaaacgcca aatggtgttg
      481 tcgagtacga gggtagcgcc agaattgacg gcgtaccggg tactgccgca ccggttgcgc
      541 tcactttcct gaatgccgct ggaacgaaaa ctggaaaagt tttcccgact gataatcaga
      601 ttgattattt tgacgatgtc ccggtgacct gtatcgatat ggcgatgcca gtcgtcatta
      661 ttccggctga atatctggga aaaacaggtt atgaattacc ggcggaactg gatgccgaca
      721 aagcattatt agcccgcatt gaatctatcc ggctacaagc gggtaaagca atgggcttag
      781 gtgatgtcag taatatggtt atccctaaac ctgtgcttat ttctccagcg cagaaaggcg
      841 gggcaattaa tgtgcgttat tttatgccgc attcttgcca tcgcgcgctg gcgataaccg
      901 gtgctattgc tatttccagt agttgtgcat tggaaggcac cgtcacccga caaatcgtcc
      961 cttctgtagg atatggcaat atcaatattg aacaccccag tggtgcgctc gacgttcatt
     1021 taagtaatga aggtcaggat gccacgacgt tacgcgcatc tgttattcgg acaaccagaa
     1081 aaatattttc cggtgaagtt tatcttccct gaaaaaattc gttgtcagga taaggacaat
     1141 caataaagga cttctgtatg agtcatacag aaagaacagg attttaaatg aataagaaat
     1201 cgttatggaa gctaattctg atattagcga tcccatgtat tattggtttt atgccagctc
     1261 cggcaggatt aagcgaactg gcgtgggtgc tttttggtat ttacctggcg gccattgtgg
     1321 gtctggttat caagcctttc ccggaacctg tcgtactgtt aattgccgtt gctgcctcaa
     1381 tggtggtggt cggtaactta tccgacggtg cgtttaagac caccgccgta ttaagcggtt
     1441 actcttcagg tactacctgg ctggtgttct cggcgtttac cttaagcgcc gcatttgtga
     1501 ccaccggttt aggtaaactt attgcctatc tgctgattgg taaaatcggt aacaccatgc
     1561 tgggtctggg ttacgttacg gtattcctcg atctggtact ggctccggca acaccgtcta
     1621 acaccgcgcg tgcgggcggc attgtgttac cgatcatcaa cagcgtggcg gtggctttgg
     1681 ggtccgaacc ggaaaaaagt ccgcgtcgtg tcggacatta cctgatgatg tccatttaca
     1741 tggtcaccaa aaccaccagc tatatgttct ttaccgcaat ggcggggaac attctggcgc
     1801 tgaaaatgat caacgacatt ctgcacctgc aaattagctg gggtggatgg gcgctggccg
     1861 ccggattgcc gggcatcatt atgctgctgg tcaccccgct ggtaatttac accatgtatc
     1921 caccagaaat taaaaaggtg gataacaaaa ccatcgctaa agcgggcctt gccgaactgg
     1981 gaccgatgaa aatccgcgaa aaaatgctgc tcggtgtctt tgtgctggcg ctactgggct
     2041 ggattttcag taagtctctg ggggttgatg aatccactgt ggcaatcgtt gttatggcga
     2101 ccatgctgct gctgggtatc gttacctggg aagacgtggt taaaaataaa ggcggctgga
     2161 ataccttaat ctggtacggc ggtattatcg gcttaagctc cttattatcg aaagttaaat
     2221 ttttcgaatg gttagctgaa gtctttaaaa ataacctggc atttgatggt cacggtaacg
     2281 ttgctttctt cgttattatt ttcctcagca tcatcgtgcg ttatttcttc gcttccggta
     2341 gtgcctatat cgttgctatg ttacctgtat ttgccatgct ggcgaacgtc tccggcgcac
     2401 cgttaatgtt aaccgcgctg gcactgttgt tctccaactc ctatggcggc atggttactc
     2461 actatggcgg cgcggcaggt ccggtcatct ttggcgtggg ttacaacgat attaaatcct
     2521 ggtggttggt cggtgcggta ctgacgatat taaccttctt ggtgcatatc accctcggcg
     2581 tgtggtggtg gaatatgctg atcggctgga acatgctgta aatatactcg tcatacttca
     2641 agttgcatgt gctgcgtctg cgttcgctca ccccagtcac ttacttatgt aagctcctgg
     2701 ggattcactc acttgtcgcc ttcctgcaac tcgaattatt tagagtatat ccatttatta
     2761 tctttctgcg cacttcacgg tgcgcagata tctggagcat ttgatgatca agttatctga
     2821 aaaaggcgtg tttctcgcca gtaataacga aataattgcc gaagaacatt tcaccggcga
     2881 aattaaaaaa gaagaagcaa aaaaaggcac tattgcctgg tctattctct cttctcataa
     2941 tacgtccgga aatatggata aacttaaaat taagtttgat tcattagcct ctcacgatat
     3001 tacctttgtt ggtattgtac agaccgctaa agcgtccgga atggaacgtt tcccgctgcc
     3061 gtatgtgctg accaactgcc ataactcact ctgcgccgtc ggcggcacca ttaacggtga
     3121 tgaccatgtt tttggtttat cggcagctca gcgttatggc ggtatttttg tgcctccgca
     3181 tattgcggtc atccatcaat atatgcgtga gatgatggct ggcgggggca aaatgatcct
     3241 cgggtcagac agccacaccc gttacggtgc attagggaca atggcagtcg gtgagggtgg
     3301 cggtgagttg gtaaaacagc tgcttaatga cacctgggat atcgactatc cgggcgtggt
     3361 tgcggtgcat ctgaccggaa aacctgcgcc gtatgtggag ccacaggatg tggcgctggc
     3421 tatcattggc gcggtgttca aaaacggtta cgtcaaaaac aaagtcatgg agttcgttgg
     3481 accgggcgtt agcgcgctct ctaccgattt ccgtaacagc gttgacgtga tgaccacgga
     3541 aaccacctgt ttaagctccg tctggcaaac tgatgaagaa gtgcataact ggctggcgct
     3601 gcacggtcgc ggtcaggatt actgccagct taaccctcaa ccgatggcgt actacgatgg
     3661 ctgcatcagc gttgatttaa gcgccatcaa accaatgatt gcgctgccgt tccacccgag
     3721 caacgtgtat gaaatcgaca cactgaacca gaacctgacc gacattctgc gtgagattga
     3781 aattgagtcc gaacgcgtgg cgcacggtaa agccaaactc tcgctgctgg ataaagtgga
     3841 aaatggtcgc ctgaaagtgc agcaggggat tatcgcgggc tgttctggcg gtaactacga
     3901 aaacgtcatc gcggcggcga atgcactgcg cggtcaatcc tgtggcaatg acaccttctc
     3961 gctggcggtt tacccgtcat cacagccggt gtttatggat ctcgccaaaa aaggtgtggt
     4021 agcagatttg attggcgcag gcgcaatcat cagaaccgca ttctgtggcc catgctttgg
     4081 cgcgggcgat acgccaatca acaacggttt gagtattcgc cacaccacgc gtaacttccc
     4141 gaaccgcgaa ggctctaagc cagccaatgg gcagatgtca gcggtggcgt tgatggacgc
     4201 tcgttctatc gctgcgactg cggcaaacgg tggctattta acctctgcca gcgaacttga
     4261 ttgctgggac aacgtgccgg agtacgcctt cgatgtaacg ccgtataaaa accgtgttta
     4321 tcagggcttt gtgaaagggg caactcagca accgctgatt tacggaccga acattaaaga
     4381 ctggccggaa ttgggtgcgc tgactgacaa tatcgtcctg aaagtgtgct cgaagatcct
     4441 cgacgaagtc accaccaccg acgaactgat tccttccggt gaaacctctt cttatcgttc
     4501 aaatccgatt ggtctggcgg ggaatgtcag cgagctgaca gaggtgtttg cgcgcattaa
     4561 gcagattgct ggtcaggagc atattgatcc gctgcaaact gaaattggta gcatggtcta
     4621 tgcggtgaaa ccaggcgatg gttctgcgcg tgaacaggcg gcgagctgcc agcgtgtgat
     4681 tggcggtctg gcgaatattg ccgaggagta cgcgactaaa cgctaccgtt ctaacgtcat
     4741 caactggggg atgttaccgc tgcagatggc ggaagtgcca acctttgaag tgggggatta
     4801 catttacatc cctggcatta aagcggcgct ggataatccg ggtacgacgt ttaaaggtta
     4861 tgtgatccat gaagatgcgc cggtaacgga aattacgctt tacatggaaa gcctgacggc
     4921 tgaagagcgc gagattatca aggcgggtag tttgattaac ttcaataaaa accgtcagat
     4981 gtaaaaagcg ccatgtgaat gtaggtcgca ttcggcactt attgtcggat gcgatgctgg
     5041 cgcatcttat ccgacctaca aatcgcatcg aaccgtaggc cggataaggc gtttacgccg
     5101 catccggcaa atagttaata gctcttactt cttctcctct gcaaccactt tgctgcccac
     5161 gccgcggtta ttgtattccc acatgcggtt gtagttagtg tcattcagat tgcgctgtat
     5221 ttcgtcgtta tcatctacgc tgccggtatt acccgcaaac ggacgattag agatcaccgc
     5281 atcggcccac ggtttagccg tgttaaaacc ttcgttgatg gcgctatcac ggatcaccac
     5341 ctgaccgttg gtattagcat caacatccag cgagcggccc agttgcgcca cgccatcacc
     5401 ggaagcattg aaacggctgt ttacggcgag gaaaccgtaa tagatgttag acagcgtagc
     5461 cggtgcaaac acatatgctt cttgctgggt acgtgagttc accacgcgga attcggtgtt
     5521 atcgaacacc actgcgccgc gaccagaaac gatatccaca tccccttcaa tatagctgtt
     5581 ggtcaccagc gtacgcggct gacggttggt ttccagacgg ttctgcacac cgctgttggt
     5641 gacaaagaag gtgttctgac gaccgagaat gttaacgttg ttaatctgta cttggtcacc
     5701 gtcagtacgc agtgctaccg ccggatggtt acctgcatct acgctatcgc ccagcgtgtt
     5761 ttcgatggtc agattttgca gttgcagtcc attgttttgt gaccagaaga ccgcagaaca
     5821 cagaacaccg atactgtcgc tgcgtttgct ctggcagcta tcgtacatat accacgctgg
     5881 tttacctggc atatatttgc cgcgcgggtt gacgtcgtga cgccagtcgg cagggctcat
     5941 gcccccatca agggaaagcc caatcttcac atcaatcggt ttttcacctg taccgtacag
     6001 agtgattcca cctggagcag cagggacata caccgttccc tgatactcac caggcatcac
     6061 ggcaatatac tggcgcttgt tggtgcgctt gataattgcc gcatctaccg ccgcctgaat
     6121 cgtggtatgc gttacacctt gagtacccgc cgggccgaca acaaagtcag gttgcgcagg
     6181 cagggttatc ggggacggat tccacgctgc agcacctggt gtcagggatg caaaatagtg
     6241 ttgcgcatcg aaattcagcg cttcttttgc cgacagaatc gggcgagaag aggtaccagg
     6301 cgcagtttga tcagaaggac gttgatcggg tggtgttgag ctacaggcgg tcagcgtcac
     6361 gccaaaagcc aatgccagcg ccagacggga aactgaaaat gtgttcacag gttgctccgg
     6421 gctatgaaat agaaaaatga atccgttgaa gcctgctttt ttatactaag ttggcattat
     6481 aaaaaagcat tgcttatcaa tttgttgcaa cgaacaggtc attatcagtc aaaataaaat
     6541 cattatttga tttcaatttt gtcccactcc ctgcctctgt catcacgata ctgtgatgcc
     6601 atggtgtccg acttgtgccc gagaagatgt tgagcaaact tatcgcttat ctgcttctca
     6661 tagagtcttg cagacaaact gcgcaactcg tgaaaggtag gtggatcccc ttcgaaggaa
     6721 agacctgatg cttttcgtgc gcgcataaaa taccttgata ctgtgccgga tgaaagcggt
     6781 tcacgacgag tagatgcaat tatggtttct ccgccaagaa tctctttgca tttatcaagt
     6841 gtttccttca ttgatatccc gagagcatca acatgcaatg ttgtagggat ggcaattttt
     6901 acgcctgttt tgctttgctc gacataaaga tatccatcta cgatatcaga ccacttcatt
     6961 tcgcataaat caccaactcg ttgcccggta acaacagcca gttccattgc aagtctgagc
     7021 caacatggtg atgattctgc tgcttgataa attttcaggt attcgtcagc cgtaagtctt
     7081 gatctcctta cctctgattt tgctgcgcga gtggcagcga ccgggtttgt tgttatatgg
     7141 ccttcagcta ttgcctctcg gaatgcatcg ctcagtgttg atctgattaa cttggctgat
     7201 gccgccttgc cctcgtctat gtatccattg agcattgccg caatttcttt tgtggtgatg
     7261 tcttcaagtg gagcatcagg cagccccctc cttattgctt taattttgct catgtaattt
     7321 atgagtgtct tctgcttgat tcctctgctg gcgaggattt tttcgtagcg atcaagccat
     7381 gaatgtaacg taacagaatt atcactgttg attctcgctg tcagaggctt gtgtttgtgt
     7441 cctgaaaata actcaatgtt ggcctgtata gcttcagtga ttgaggtgta ctggcaatag
     7501 cggacactac catttgttct ttttttaagc ag
//
LOCUS       DAZPCS010000169         7477 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZPCS010000169 DAZPCS010000000
VERSION     DAZPCS010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7477
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.171"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..302
                     /locus_tag="ACHUQ7_003445"
     CDS             <1..302
                     /locus_tag="ACHUQ7_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HFR1136705.1"
                     /translation="SYLKAQFEKLYQRDMKKYLGEMKRLADGGEMNFDGYLDKMSHLV
                     NEGTLDPDILSKMRDAAPQLISFAKSFDPTSKEEIKILTDTSKLIYDLFGVKSEK"
     gene            665..1702
                     /gene="dusA"
                     /locus_tag="ACHUQ7_003446"
     CDS             665..1702
                     /gene="dusA"
                     /locus_tag="ACHUQ7_003446"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFR1136706.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            1836..2078
                     /gene="pspG"
                     /locus_tag="ACHUQ7_003447"
     CDS             1836..2078
                     /gene="pspG"
                     /locus_tag="ACHUQ7_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFR1136707.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(2244..3227)
                     /locus_tag="ACHUQ7_003448"
     CDS             complement(2244..3227)
                     /locus_tag="ACHUQ7_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFR1136708.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGIGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            3310..4725
                     /gene="dnaB"
                     /locus_tag="ACHUQ7_003449"
     CDS             3310..4725
                     /gene="dnaB"
                     /locus_tag="ACHUQ7_003449"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFR1136709.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            4778..5857
                     /gene="alr"
                     /locus_tag="ACHUQ7_003450"
     CDS             4778..5857
                     /gene="alr"
                     /locus_tag="ACHUQ7_003450"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFR1136710.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            6110..7303
                     /gene="tyrB"
                     /locus_tag="ACHUQ7_003451"
     CDS             6110..7303
                     /gene="tyrB"
                     /locus_tag="ACHUQ7_003451"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFR1136711.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
BASE COUNT         1797 a         1798 c         1976 g         1906 t
ORIGIN      
        1 agagctacct gaaagctcag tttgaaaaat tatatcaacg tgatatgaag aagtacctgg
       61 gagagatgaa gcgtctggca gatgggggag aaatgaattt tgatggctat ctggataaga
      121 tgtcacatct ggtgaatgaa ggaacgctcg atcctgatat tttaagcaaa atgcgagatg
      181 ctgcaccaca attaattagc ttcgcgaagt cgtttgaccc aacctcaaag gaagagatta
      241 aaatacttac agacacttct aaattaattt atgacttgtt cggggttaaa tcggagaaat
      301 aatatgtgaa gttcttcgat agtatggaag gcattatata aaaggaccca atatttattg
      361 ggttcttttt tctctatcaa tgctattagc agggagatat atcaccagag tttaatgtgt
      421 gattttttat ttatcgtcga acctggattg tttatcattg gccttaacaa agttaacggc
      481 taataagatt atttccatca cttcgtgaga gcttcatgcc ttgagaggat ctcaattttc
      541 ttttgcaatg agacaggcgc ttcctgttgt tatggtatag taccccgcta ttgggcctcc
      601 tgaacagtga agctgaataa cataacccca tgatatatcg ataaaataat ctctacattt
      661 gaaaatgcac ggtaattctg aaatgcaaaa aatcaaccaa accagcgcaa tgcctgaaaa
      721 aactgacgtt cactggagtg gtcggtttag tgttgcacca atgctcgact ggacggacag
      781 acattgccgc tatttcttgc gtctgctttc ccgcaatacg ttgctttata ccgaaatggt
      841 gaccacaggg gcgataattc acggtaaagg tgattatctg gcgtacagtg aagaagaaca
      901 tccggtagcg ttgcaactgg gcggtagcga tccggcggcg ctggcgcagt gtgcaaagct
      961 ggcagaagcg cgcggatatg atgagatcaa cctgaatgtc ggctgcccgt ctgaccgggt
     1021 gcagaacggc atgtttggtg cgtgtctgat gggtaatgcg cagctggttg ccgactgcgt
     1081 gaaagcgatg cgcgatgtgg tgtcgattcc ggtgacggtg aaaacgcgta ttggcatcga
     1141 cgaccaggac agctatgaat ttctctgcga tttcatcaat accgtttccg gcaaaggcga
     1201 gtgtgagatg ttcatcatcc acgcacgtaa agcctggctt tcggggttaa gcccgaaaga
     1261 aaaccgtgaa atcccgccgc tcgattatcc gcgtgtgtat caactgaagc gtgacttccc
     1321 gcatctgaca atgtcgatta acggtggtat caagtcgctg gaagaagcta aagcgcattt
     1381 gcaacatatg gatggcgtga tggtcgggcg cgaggcgtat cagaatccgg gtattctggc
     1441 ggcggtagac cgggagatct ttggttcctc ggataccgat gccgatccgg tggcagtagt
     1501 gcgtgccatg tatccgtaca ttgagcatga actcagccag gggacgtatc tcggccatat
     1561 tatccggcat atgctgggtt tgttccaggg tattcctggc gcgcggcagt ggcggcgtta
     1621 tttaagtgaa aatgcccata aagcgggtgc tgacattaac gtgctggaac acgcgctcaa
     1681 actggtggcg gataagcgtt aacttttcac caaaaagtag tcaaattcac cacgccctgc
     1741 gcaccgtcgc ggggcgtttt gctgttaaat caatagatta tttttggcat gattcttgta
     1801 atgccagcaa gagatttcat atttgggaga gcatcatgct ggaactactt tttgtgattg
     1861 gcttttttgt catgctgatg gtcaccggcg tttcgttgct gggcattatc gccgcgctgg
     1921 ttgtggcgac ggccattatg ttcctcggcg gtatgctggc attgatgatt aagttgctgc
     1981 cgtggttact actggcgatt gcggtggtgt gggttattaa ggcgattaaa gcaccaaaaa
     2041 tgccgaaata tcagcgttat gaccgctggc gttactaagg gattgtgcgg atgatcacaa
     2101 cctaaggttt tatccttaga acaaaatagg aattgataat caaatctgtc actattgcgc
     2161 ctctaacaga ttcatcgtgc tgtaccctac atacagccga actataaaaa gaaagggctt
     2221 cccaggtgga agccctattt cttttatgga atcagcaggc tggaaccttg cgtcgcccgg
     2281 ctttccagaa tctcatgcgc acgctgcgca tccttcagcg gatatttctg ctgctcggcg
     2341 acatcgacct taatcacacc gctggcaatc aaagagaaca gttcattact ggcctcggtt
     2401 aattcctccc gcgtggtgat atagccttgc agggaagggc gtgtcacata caacgagcct
     2461 ttttgattga gaatgcctaa gttcacaccg gtaaccgcac ctgatgagtt gccaaaactg
     2521 accattaagc cgcggcgttg caggcaatcc agcgaccgtt cccaggtgtc tctgcccacg
     2581 gaatcgtaca ctacgcgcac tttcttaccg ccggtgatct ctttcaatcg ctcgaccaga
     2641 ttctcttcac gatagttaat aacctgccac gcgccggctt ttagcgcgct ctgcgctttt
     2701 tgcgcggttc ctacggtgcc gatgagtttc gcgcccaggg cttttgccca ctggcaggca
     2761 attaagccaa tgccgccagc cgctgcgtgg aacaggaact gttcatcggg tttaatttca
     2821 taggttttgc gcagcagata ataaaccgtt aagcctttca ggaaggatgc cgcagcttgc
     2881 tcaaaagaaa ttgccgcagg cagaatcgcc gctttatccg cattaatgtt atgcacagag
     2941 ctgtaagcgc ctaacgccga ctgcgcatag actacatgat cgccagcctt aatatgcttt
     3001 acaccactgc cgactttact cacgatgcct gccgcttcgg tacctaatcc gctgggtagc
     3061 gatggcggcg ggtaaaggcc gctgcggata tacgtgtcga taaaattgat gccgatggct
     3121 ttattttcga cctggatttc attctccgcc ggatcggcag gagtgaactc tacggcttga
     3181 agtacttccg ggccaccgtg cttgtgaaat tcaattcgtg ttgccatgtg tcctccagaa
     3241 cgtatcgtca gggtctgctt catatggtaa agtttcgacc cattctttat ttcggtaact
     3301 ccattcacta tggcaggaaa taaacccttc aacaaacagc aggctgaacc ccgcgaacgc
     3361 gatccacaag ttgccgggct gaaagtgcct ccgcactcga tcgaagcgga gcagtcggtg
     3421 ttgggcggtt taatgctgga taacgaacgc tgggatgatg tagccgagcg tgtggtagca
     3481 gacgattttt acacccgccc acaccgtcat atctttactg aaatggcgcg tttgcaggaa
     3541 agcggtagtc ctatcgatct gattaccctt gcggaatcgt tggaacgcca ggggcaactc
     3601 gatagcgtcg gtggttttgc ttatctggca gagctgtcaa aaaatacgcc aagtgcggcg
     3661 aacatcagtg cttatgcgga catcgtgcgt gaacgtgccg ttgtccgtga gatgatctcg
     3721 gttgcgaatg agattgctga agccggtttt gatccgcagg ggcgtaccag cgaagatctg
     3781 ctggaccttg ctgaatcccg cgtctttaaa attgccgaaa gtcgtgcaaa caaagacgaa
     3841 gggccgaaga acatcgccga tgtgctcgac gcaaccgtgg cgcgtattga gcagttgttt
     3901 cagcagccac acgatggcgt taccggggta aacaccggtt atgacgatct caacaaaaaa
     3961 accgctggct tgcagccgtc ggatttgatc atcgtcgccg cgcgtccgtc gatgggtaaa
     4021 acaacatttg cgatgaacct cgtcgaaaac gcggcaatgt tgcaggataa accagtactt
     4081 atcttctcgc tggagatgcc atcagaacag atcatgatgc gttctctggc gtcgctgtcg
     4141 cgcgttgacc agactaaaat ccgtaccggg cagctcgatg atgaagactg ggcgcgcatt
     4201 tccggcacca tgggtatttt gctcgaaaaa cgcaatatct atatcgatga ctcttccggc
     4261 ctgacgccaa cggaagtgcg ttcccgcgca cgccgtattg cccgtgaaca cggcggcatc
     4321 gggcttatca tgatcgacta cctgcaactg atgcgcgtac cggcgctttc cgataaccgt
     4381 acgctggaaa ttgcagaaat ctcccgctcg ctgaaagcac tggcgaaaga actgaacgtg
     4441 ccggtggtgg cgctgtccca gttgaaccgt tctctggaac aacgtgccga caaacgtccg
     4501 gtcaactccg acctgcgtga atctggctct atcgagcagg atgcggactt gatcatgttt
     4561 atctatcgtg atgaggtgta tcacgaaaac agtgatttaa aaggcatcgc ggaaattatt
     4621 atcggtaaac aacgtaacgg cccaatcggg acggtacgcc tgacctttaa cggtcaatgg
     4681 tcgcgcttcg acaactatgc ggggccgcag tacgacgacg aataataatt attttatgaa
     4741 ttaggtaatt aaagcaaaca attatcaagg aacacaaatg caagcggcaa ctgttgtgat
     4801 taaccgccgc gctctgcgac acaacctgca acgtcttcgt gaactggcac ctgccagtaa
     4861 aatggttgcg gtggtgaaag cgaacgctta tggtcacggt cttcttgaga ccgcgcgaac
     4921 gctccccgat gctgacgcct ttggcgtagc ccgtctcgaa gaagctctgc gactgcgtgc
     4981 ggggggaatc accaaacctg tactgttact cgaaggcttt tttgatgcca gagatctgcc
     5041 gacgatttct gcgcaacatt ttcataccgc cgtgcataac gaagaacagc tggctgcgct
     5101 ggaagaggct agcttggacg agccggttac cgtctggatg aaactcgata ccggtatgca
     5161 ccgtctgggc gtaaggccgg aacaggctga ggcgttttat catcgcctga cccagtgcaa
     5221 aaacgttcgt cagccggtga atatcgtcag ccattttgcg cgcgcggatg aaccaaaatg
     5281 tggcgcaacc gagaaacaac tcgctatctt taataccttt tgcgaaggca aacctggtca
     5341 acgttccatt gccgcgtcgg gtggcattct gctgtggcca cagtcgcatt ttgactgggt
     5401 gcgcccgggc atcattcttt atggcgtctc gccgctggaa gatcgctcca ccggtgccga
     5461 ttttggctgt cagccagtga tgtcactaac ctccagcctg attgccgtgc gtgagcataa
     5521 agccggagag cctgttggtt atggtggaac ctgggtaagc gaacgtgata cccgtcttgg
     5581 cgtagtcgcg atgggctatg gcgatggtta tccgcgcgcc gcgccgtccg gtacgccagt
     5641 gctggtgaac ggtcgcgaag taccgattgt cgggcgcgtg gcgatggata tgatctgcgt
     5701 agacttaggt ccacaggcgc aggacaaagc cggggatccg gtcattttat ggggcgaagg
     5761 tttgcccgta gaacgtatcg ctgaaatgac gaaagtaagc gcttacgaac ttattacgcg
     5821 cctgacttca agggtcgcga tgaaatacgt ggattaatcg ttctgtaata tttgattgtc
     5881 tgtgccggat gcggcgtgaa tgccttatcc ggccaataaa atcctaaaaa ttcaataagt
     5941 tgatgttctt tcatgctctt ataaaggtcg tgcctctggc ggatgtacgt ttatcattag
     6001 tctcactctg ttgctaattg ccgttcgctc ctgaacatcc actcgatctt cgccttcttc
     6061 cggtttattg tgttttaacc acctgcccgt aaacctggag aaccatcgcg tgtttcaaaa
     6121 agttgacgcc tacgctggcg acccgattct tacgcttatg gagcgtttta aagaagaccc
     6181 tcgcagcgac aaagtgaatt taagtatcgg tctgtactac aacgaagacg gaattattcc
     6241 acaattgaaa gccgtggcgg aggcggaagc gcgcctgaat gcgcagcccc atggcgcttc
     6301 gctttattta ccgatggaag ggcttaacag ctatcgccat gccattgcgc cgctgctgtt
     6361 tggtgcggac catccggtac tgcaacaaca gcgcgtagca accattcaaa cccttggcgg
     6421 ctcaggggca ttgaaagtgg gcgcggattt cctgaaacgc tacttcccgg aatcaggcgt
     6481 ctgggtcagc gatcctactt gggaaaacca cgtagcaata ttcgccgggg ctggattcga
     6541 agtgagtact tacccctggt atgacgaagt gactaacagc gtgcgcttta atgacctgtt
     6601 ggcgacgctg aaaacattac ctgcccgcag tattgtgttg ctgcatccat gttgccacaa
     6661 cccaacgggt gccgatctca ctaatgatca gtgggatgcg gtgattgaaa ttctcaaagc
     6721 ccgcgagctt atcccattcc tcgatattgc ctatcaagga tttagtgccg gtatggaaga
     6781 ggatgcctac gctattcgcg ccattgccag tgctggatta cccgctctgg tgagcaattc
     6841 gttctcgaaa attttctccc tttacggcga gcgcgtcggc ggactttctg ttctgtgtga
     6901 agatgccgaa gccgctggcc gcgtactggg gcaattgaaa gcaacagttc gccgcaacta
     6961 ctccagcccg ccgaattttg gtgcgcaggt ggtggctgca gtgctgaatg acgaggcatt
     7021 gaaagccagc tggctggcgg aagtagaaga gatgcgtact cgcattctgg caatgcgtca
     7081 ggaattggtg aaggtattaa gcacagagat gccagaacgc aatttcgatt atctgcttaa
     7141 tcagcgcggc atgttcagtt ataccggttt aagtgccgct caggttgacc gactacgtga
     7201 agaatttggt gtctatctca tcgccagcgg tcgcatgtgt gtcgccgggt taaatacggc
     7261 aaatgtacaa cgtgtggcaa aggcgtttgc tgcggtgatg taatgcagga aagcaggctg
     7321 gagttaccca gcctgcagtg aaattaaact gtcgtcgctt tcactatttc tttgtagatg
     7381 atttttttga tgccgttgtt ctacgtgaga gataataaaa cgttgtcagt tcttttattg
     7441 ttaagtttat cccaattatc tggaattcct ttcccta
//
LOCUS       DAZPCS010000170         7363 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZPCS010000170 DAZPCS010000000
VERSION     DAZPCS010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7363
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.172"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(13..1227)
                     /gene="rspA"
                     /locus_tag="ACHUQ7_003452"
     CDS             complement(13..1227)
                     /gene="rspA"
                     /locus_tag="ACHUQ7_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HFR1136712.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNR"
     gene            complement(1433..1759)
                     /locus_tag="ACHUQ7_003453"
     CDS             complement(1433..1759)
                     /locus_tag="ACHUQ7_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HFR1136713.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            1894..2235
                     /locus_tag="ACHUQ7_003454"
     CDS             1894..2235
                     /locus_tag="ACHUQ7_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HFR1136714.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            2301..2830
                     /gene="speG"
                     /locus_tag="ACHUQ7_003455"
                     /pseudo
     CDS             2301..2830
                     /gene="speG"
                     /locus_tag="ACHUQ7_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(2833..3543)
                     /locus_tag="ACHUQ7_003456"
     CDS             complement(2833..3543)
                     /locus_tag="ACHUQ7_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HFR1136715.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            3651..3956
                     /locus_tag="ACHUQ7_003457"
     CDS             3651..3956
                     /locus_tag="ACHUQ7_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HFR1136716.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            4155..5568
                     /locus_tag="ACHUQ7_003458"
                     /pseudo
     CDS             4155..5568
                     /locus_tag="ACHUQ7_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex A
                     subunit"
     gene            complement(6298..7287)
                     /locus_tag="ACHUQ7_003459"
     CDS             complement(6298..7287)
                     /locus_tag="ACHUQ7_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1136717.1"
                     /translation="MTTIKDAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQENIPFPVEVLPTRNSLPTPIIAHTLADRSVVVPVRGGKRPEVVT
                     VPSGQEIVVEPIEQAILISEQTKLWDAKSSTGFTQGTLQQDAMNICENVVRTINARMV
                     DVLESSKLLKTVELPVLTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESQLKA
                     LSRLAAKQGFSGEDAIVDMLGTDIAYYNGEDKGVFMLAKRFTALSFGCFRHDGENITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLKFTKTEA"
BASE COUNT         1945 a         1873 c         1762 g         1783 t
ORIGIN      
        1 ttatcttact ccttacctgt tccacagcgt gccatcttcc agacgtgcga ctggtagata
       61 agcaggttca tagggatatt tcgccgccag cttttcatcg aattcgatac caagacccgg
      121 tttgtctccc ggatgcatat agccgttatc gaaagtccag ttgtgcggga agacttcgag
      181 catttgttcg gaataaccca tgtattcctg gacaccgaaa ttggggaccc acagatcaaa
      241 gtgcagcgcc gcagccatgc agactggtga caaatcggaa ggaccgtgtg agccagtacg
      301 tacctgatac agcgaagcaa aatcggcaat ccggcgcata ccggtaattc cgcctgcatg
      361 ggtcagcgtg gtgcggatat aatcgatgag ttgctcttca atcagttgtt tgcagtccca
      421 gatgctgttg aagacttcac ccactgcgat gggtgtgacg gtatgttggc gaatgagacg
      481 gaagcattct tggttttccg ctggcgtcgg gtcttccatc cagaacatgc gataatcttc
      541 aatgctttta ccaaagcgcg ccgcttcaat aggcgttaag cgatggtgca tgtcatgcag
      601 caaatgttca ttaaaaccaa acttgttacg taccgcgtca aacaatttcg gcatgaaatc
      661 gaggtatttc tccgtcgacc acagctgctc ttccggccac tgtcctttgg ttgcgggttc
      721 ataagccaga cctttacctt tcgacatgcc gtaggtggtt ttcataccag ggattccgca
      781 ctgcacgcgg atggctttga atcccagctc ctgatgacgg gcataatcat ccagagcttc
      841 atcaatactg tgaccggtgg tatggcaata aaccatcacc ccttcacgag acgcgccgcc
      901 gagtaactgg taaagcggca tgttggcagc tttggcttta atatcccaca gcgccatatc
      961 aaccgctgaa atggccgaca tcgtaaccgg accgcgacgc cagtaagcac ctttatagaa
     1021 aaactgccag atatcttcga tacggtgcgc atcgcgacca ataagccgcg gacaaagatg
     1081 atcctgcaaa taagaggcca cggaaagctc acgtccattg agggtggcat ccccaaggcc
     1141 cgtaataccg tcctcagtgg tgatttttaa tgtgacgaaa ttacgccccg gacaggtaac
     1201 aaaaacttca gcctttacga tcttcatgtt cgattccttg catcgcttgt cgtgatgcat
     1261 gaaatctacg caactgagct actgccatac aagtatgaag atcgaaaaaa gccggagtga
     1321 tcacaaaaaa aggcgtatat ttgcgctgtg aatggttgac aaaagatgaa atagaatacc
     1381 ttttgtcagc tgacacttcc tcgtatctta ttgataaaat ggatttatgt tcctacgtgc
     1441 gcccccagcc cgcaacaatg atcaacattc cgcaaagcgc aatcaacgca cccgtccagt
     1501 cataaagact tagtttcacg ccatccacaa cgcgcagcca catcaatgcc gtgcagacat
     1561 aaacgccacc ataagccgcg taaacacgcc cactcgccgc aggatgcaac gttaacaacc
     1621 agacaaacag cgccagtgaa atccccgccg gaagcaacag ccagatactg gcgtttcgtt
     1681 ttaaccacaa ccagggcaga aagcatccaa taatttcaca cagcgcagta gcaaaaaata
     1741 gtaacgttgt tttaatcatc tttgtcactt attgacatca tgtatagtta tagggcgaca
     1801 taatatcatc aatataaaca tcctcctggt acgttttgcg ttcgcagtgg atggtgtaga
     1861 atcaccttta atcattcata cagggaatga attatgaaaa tcactctcag caaacgaatc
     1921 ggcctgctcg cttttctgct gccttgcgca ctggcattga gcacaactgt tcatgccgaa
     1981 actaacaaac tggtgattga gtctggcgac agtgcacaaa gccgccagca cgccgctatg
     2041 gaaaaagagc aatggaatga cacgcgcaat ctgcgccaga aagtgaataa acgcactgaa
     2101 aaagagtggg ataaagccga cgccgctttt gataaccgcg ataaatgtga gcaaagtgcc
     2161 aacatcaatg cctactggga gcccaatact ttgcgctgcc tggaccgtcg aactggccgc
     2221 gttattaccc cctaacctgt tattgattta aggaatgtaa ggacacgtta tgccaagcgc
     2281 ccacagttaa gctacgcccg ctggagcgtg aagatttacg ctatgtacat caactcgaca
     2341 ataacgccag tgtgatgcgt tactggtttg aggaacccta cgaagccttt gttgaactct
     2401 ctgatctgta tgataagcgc atattcacga tcagagcgaa cggcgctttg tggtggaatg
     2461 tgacggcgaa aaagccggtc tggtggagct ggtggaaatt aaccatgttc atcgccgggc
     2521 agaatttcag ataattatct ccccggagta tcaggggaaa ggtctggcaa cccgtgccgc
     2581 caaattagca atggactatg gcttcaccgt tctcaatctc tataagctgt atctgatcgt
     2641 cgataaagag aatgaaaaag cgattcacat ttaccgcaag cttggcttta cggttgaagg
     2701 tgaattgatg catgagtttt ttattaatgg tcaatatcgt aatgccattc gcatgtgtat
     2761 attccagcat cagtatctgg cagagcacaa aacaccgggt cagactctcc tgaagccgac
     2821 cgcacaatag cattaataat aatcgatcgt atttttgatg gtgtaaaccc gttcgacggc
     2881 gggttttact ccttcatcaa caatgatcag ctgacagtcc actggattgg cgtgactgtc
     2941 atattcacag ctctgtttta cattaccaac tcgttgatta tttagtaaag taaccgctgt
     3001 gtaatctaat tttttgatcg gatccgttga tggcgtggcg ctgacagata atgttttgtc
     3061 gttacttttc gtggttttac ccagcggata cccctgatca tcatagcgat attccatctg
     3121 catttgtttg ctgctggctt taatcacgaa gccattatca tcggtttccc aactcacccc
     3181 ggcagaaggt aattctgcta gctggcattt tccctgtaaa cgtactctct tctccagcgt
     3241 ctcggcatca cggtaataat tggcgtccag taccagagcg accacggtat tattttccag
     3301 atccagtaat tcgagtgaat caaaacagcc ttcttccgac aaagtcccag aaacacgttt
     3361 cgtcacttca ccttgctcat ccattaatgt ctgagtgaaa tcttttaccg gaccacgcag
     3421 cggatcaaaa tcgaattcat tagagaaact ggccatctca ggggtaaatg aaagtgttac
     3481 ctctgtgcgg tcacatcctg tgaggaatat cgcgagtaag catggtagta atttgtattt
     3541 cacaacagtc accagagagt agagatgatt ctcaatcata gtagcaaata cagtacttta
     3601 cacgttaaat gctatgctta aagaagttat cttcgcgtaa ggagcttatg atgaaactct
     3661 caacctgctg tgccgcgctt ctgctcgccc tcgcctcacc cgcggtactc gccgcgccgg
     3721 gatcttgtga gcgcatacag agcgatatat cacagcgcat tatcaataat ggcgtacccg
     3781 aaagcagctt cacgttaagt attgtaccca atgaccaggt tgatcagcct gattcccagg
     3841 tcgtcgggca ttgcgctaat gatacgcata aaattctcta caaccgcacc accagcggta
     3901 acgtctctgc tcccgcgcag tctacccagg atggcgcgcc tgccgaaccg cagtaacaca
     3961 ttatcgactg aacgccggat atgacacatc cggcgatttg aacatacaac ataatctcac
     4021 cttattactc atacccttct attgatgtgg attaataatt cttaacccaa aatgggtaga
     4081 ctccctctat tgttagcgcg ctaaatattc aatgtataaa catttatata acaataaaga
     4141 acagggagtg agttatgtcc aaaaatgacc gaatggtggg catcagccgc agaacactcg
     4201 ttaaatcaac cgcgacaggt tctctggcgc tggctgcagg cggtttttct ttaccgttca
     4261 ccctgcgcag tgcagcggca gcggtacaac aggccagcga aaaagtgatc tggggttcct
     4321 gttccgtcaa ctgtggtagc cgctgtgcac ttcgtctaca tgttaaagat aatgaagtga
     4381 cctgggtgga aactgacaat accggcagcg atgagtacgg caaccatcag ggtacgcgcc
     4441 tgtttgcgcg gtcgctccat ccgccggcgt attaatcatc ccgatcgctt gaatgaccca
     4501 atgaaacgcg tgggtaaacg cggtgaaggc aaattcgaac ggattagctg ggatgaagcc
     4561 ctggatacta tcgccagtag cctgaaaaaa accgtcgaac aatatggcaa tgaagctgta
     4621 tatattcagt actcttcggg gattgttggc ggcaatatga cccgctcttc gccatcagcc
     4681 tcggcggtca aacgcctgat gaactgctac ggcggttcac tcaatcagta tggctcctac
     4741 agcaccgcgc aaatttcctg tgccatgccc tacacctacg gcagtaatga tggcaacagc
     4801 accacggata ttgaaaacag caagctggtc gtgatgtttg gtaacaaccc ggcagaaacc
     4861 cgcatgagcg gtggtggcat cacttatctt cttgaaaaag cgcgcgagaa atcgaacgcc
     4921 aaaatgattg ttatcgatcc gcgatatacc gatacggctg ccggtcgtga agaggaatgg
     4981 ctccctattc gcccgggcac cgatgccgcg ctggttgcgg gtattgcctg ggtattgatt
     5041 aacgaaaatc tcgttgatca accttttctc gataaatact gcgtcggcta tgacgaaaaa
     5101 accttacccg cagatgcacc caaaaatggt cactataaag cctatattct tggtgaaggt
     5161 gacgataaaa cagcaaaaac gccgcagtgg gcttcgcaaa ttaccggtat cccggtagac
     5221 cgtatcatca aactggcgcg tgaaattggc acaacaaaac ccgcttatat ctgctagggc
     5281 tgggggccgc aacgccaggc aaacggcgag ctgactgcac gcgctattgc tatgttacct
     5341 attttgacgg gcaatgtcgg catcagcggc ggaaatagtg gcgcgcgtga atcgacctat
     5401 accattacga tagaacgcct accggtgctg gataatccgg tcaaaacgtc aatctcctgc
     5461 ttcagctgga cggatgctat cgatcatggc ccgcaaatga cggcactccg cgacggtgtc
     5521 cggggcaaag ataaactgga tgtgcccatt aagttcatct ggaactacaa ggacttgaag
     5581 cggaccctta gtaggcctga ggtcctgcat tgagtcaaga ccttgtgtca ttctttgtgc
     5641 cagacttgtg tcattagacg ggatgtctag acggctggtc aggaccctcc ggtggcctcg
     5701 gtctggactt gaggcgggct atgggggaag tctgctggct taatgtatta gatggtcatt
     5761 cacatttttc tgtcagaaac catcggtccc actgtgagta caaacctaag cccccagaag
     5821 cccacccaca gccttagaca gagtgtcgaa tacattcaca ccgagtcgac cggagaggac
     5881 catatagagg cccacagaga ggaacacgag tttatagacc agagtcagga gggaccttag
     5941 gtcttcggaa gtcatggaca taaagccccc cctatcttct attgcactac cgcactagac
     6001 aactagtaca aaacaaaggc ccagaccatt ggtccagacc gtagttagtt aatcacagtg
     6061 acaagccacc acctgaagtc ctgctgatat tcagtccaga cttacctctg ctcatggtct
     6121 tacctccctc ggtctcctga ccttgggtat cgactacagc ttgctgagcg tcatctggag
     6181 gagctacagg aggagctaca ggaggcggga ccgaagcccc accttgagtc ccaccaccac
     6241 ctgtcaggcc cttggcgatg gacctgaagc ctttactaat gctcttaccc attggtctta
     6301 cgcttcggtc ttggtgaact tcaggcgttt gactacaggc aggacggcag tagctgagcc
     6361 agtgcccatc ttgatggtcg tagcagcttc agcgacaacg aacactttac cgaggatttc
     6421 caagtcgtgg gactgagagt cgccatcacg tgaaaggacg accgtaatat tctcaccgtc
     6481 gtgacggaag cagccgaagc tcagggccgt gaagcgttta gccagcatga atacaccctt
     6541 gtcttcaccg ttgtaatagg cgatgtctgt ccccagcata tcgacgatag cgtcttcacc
     6601 ggagaagcct tgcttagcag ccagacggga cagtgctttc agttggctct catgagcgat
     6661 aataccgtag tcagacacct cagaaccgaa agaagactct gtgttctcat acagagcgtc
     6721 catgatagcg tctgccttag cggtcaggct accagtcagg acaggaagct ctacggtctt
     6781 cagcagctta gaggattcca gaacgtcgac catgcgagcg ttgatggtac ggaccacatt
     6841 ctcacagatg ttcatagcgt cctgctgaag tgtgccctga gtgaatccgg tggatgactt
     6901 ggcgtcccag agcttggtct gctcagagat gagaatagcc tgctcgattg gttcgaccac
     6961 gatttcttgt ccagacggaa cggtcacgac ttcaggtcgc ttgccaccac ggacaggaac
     7021 gaccacagag cggtcagcca gagtgtgagc aatgattggg gtcggtaagc tgttacgggt
     7081 cggcaggact tctacaggga acgggatgtt ctcttgagcg tctttaagta caccgttcag
     7141 catagcctta accgtcttag ctaactggag ggcatagacc tgcgggtcag cgttagtcgg
     7201 cagaccttca ttgacctgaa tgttgtcggt atcggtgatg cgtactggct caatgccaaa
     7261 ctggaaagcg tctttaatcg tagtcataat aatcaatctc ctaaattagt ttaaatactg
     7321 tgagtcctca aaactgagga gtccctaaca tcgggtcagg gac
//
LOCUS       DAZPCS010000171         7327 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZPCS010000171 DAZPCS010000000
VERSION     DAZPCS010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7327
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.173"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..370)
                     /locus_tag="ACHUQ7_003460"
     CDS             complement(<1..370)
                     /locus_tag="ACHUQ7_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFR1136718.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVS"
     gene            580..1593
                     /gene="intI1"
                     /locus_tag="ACHUQ7_003461"
     CDS             580..1593
                     /gene="intI1"
                     /locus_tag="ACHUQ7_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HFR1136719.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            1796..2146
                     /locus_tag="ACHUQ7_003462"
                     /pseudo
     CDS             1796..2146
                     /locus_tag="ACHUQ7_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636771.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
     gene            2272..2832
                     /locus_tag="ACHUQ7_003463"
     CDS             2272..2832
                     /locus_tag="ACHUQ7_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR1136720.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            2835..5801
                     /locus_tag="ACHUQ7_003464"
     CDS             2835..5801
                     /locus_tag="ACHUQ7_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HFR1136721.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            5868..6245
                     /locus_tag="ACHUQ7_003465"
     CDS             5868..6245
                     /locus_tag="ACHUQ7_003465"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR1136722.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(6446..7105)
                     /locus_tag="ACHUQ7_003466"
     CDS             complement(6446..7105)
                     /locus_tag="ACHUQ7_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HFR1136723.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
BASE COUNT         1616 a         2141 c         2021 g         1549 t
ORIGIN      
        1 gcgaaaccca aacaacagaa aattgcatcc acgtctttgg tgtatgattg ctgttccaga
       61 tctccattcc tttgggggtt ttatcccatt tgaatatggt tttctgatct attatttccg
      121 catcaaatgc cataagtgat aatgcgatct tgaaagttga atctggtgcc atttgcgttg
      181 cacactttgc tttattgaat tgagcaattt cagcgtttgt ggatgcatcg taaagtaaaa
      241 aacaaccttc agttccttca aataatggag atgcaacagt agagatatct gttgatgcac
      301 tggcgctgct gtagataata tttgcaatta ttaaaaaaat agcgaagttg atatgtattg
      361 tgtttttcat aataagtatt ggtttggtaa agggcttaat tttaacggct aacaattaat
      421 gaggctccgg gttcgcccaa ctttgtttta gggcgactgc cctgctgcgt aacatcgttg
      481 ctgctccata acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga
      541 ggcatagact gtaccccaaa aaaacagtca taacaagcca tgaaaaccgc cactgcgccg
      601 ttaccaccgc tgcgttcggt caaggttctg gaccagttgc gtgagcgcat acgctacttg
      661 cattacagct tacgaaccga acaggcttat gtccactggg ttcgtgcctt catccgtttc
      721 cacggtgtgc gtcacccggc aaccttgggc agcagcgaag tcgaggcatt tctgtcctgg
      781 ctggcgaacg agcgcaaggt ttcggtctcc acgcatcgtc aggcattggc ggccttgctg
      841 ttcttctacg gcaaggtgct gtgcacggat ctgccctggc ttcaggagat cggaagacct
      901 cggccgtcgc ggcgcttgcc ggtggtgctg accccggatg aagtggttcg catcctcggt
      961 tttctggaag gcgagcatcg tttgttcgcc cagcttctgt atggaacggg catgcggatc
     1021 agtgagggtt tgcaactgcg ggtcaaggat ctggatttcg atcacggcac gatcatcgtg
     1081 cgggagggca agggctccaa ggatcgggcc ttgatgttac ccgagagctt ggcacccagc
     1141 ctgcgcgagc agctgtcgcg tgcacgggca tggtggctga aggaccaggc cgagggccgc
     1201 agcggcgttg cgcttcccga cgcccttgag cggaagtatc cgcgcgccgg gcattcctgg
     1261 ccgtggttct gggtttttgc gcagcacacg cattcgaccg atccacggag cggtgtcgtg
     1321 cgtcgccatc acatgtatga ccagaccttt cagcgcgcct tcaaacgtgc cgtagaacaa
     1381 gcaggcatca cgaagcccgc cacaccgcac accctccgcc actcgttcgc gacggccttg
     1441 ctccgcagcg gttacgacat tcgaaccgtg caggatctgc tcggccattc cgacgtctct
     1501 acgacgatga tttacacgca tgtgctgaaa gttggcggtg ccggagtgcg ctcaccgctt
     1561 gatgcgctgc cgcccctcac tagtgagagg tagggcagcg caagtcaatc ctggcggatt
     1621 cactacccct gcgcgaaggc catcggtgcc gcatcgaacg gccggttgcg gaaagtcctc
     1681 cctgcgtccg ctgatggccg gcagcagccc gtcgttgcct gatggatcca acccctccgc
     1741 tgctatagtg cagtcggctt ctgacgttca gtgcagccgt cttctgaaaa cgacaatgga
     1801 ggtggtagcc gagggtgtgg aaacacccga ctgccttgcg tggttgcggc aggcgggttg
     1861 cgacacggtg cagggtttcc tgttcgccag gccgatgccg gcggcggcct tcgtcggctt
     1921 cgtcaaccaa tggaggaaca ccaccatgaa cgccaatgaa ccgagcacca gttgctgcgt
     1981 gtgctgcaag gaaatcccgc tcgatgccgc cttcacgccg gaaggggccg agtacgtgga
     2041 gcatttctgc gggctggagt gctatcagcg cttccaggcg cgggccagca ctgcgaccga
     2101 aaccagcgtc aaaccggacg cttgtgattc gccgccgtca ggttgaggca taccctaacc
     2161 tgatgtcaga tgccatgtgt aaattgcgtc aggataggat tgaattttga atttattgac
     2221 atatctcgtt gaaggtcata gagtcttccc tgacattttg cagggaattc catgactgga
     2281 cagcgcattg ggtatatcag ggtcagcacc ttcgaccaga acccggaacg gcaactggaa
     2341 ggcgtcaagg ttgatcgcgc ttttagcgac aaggcatccg gcaaggatgt caagcgtccg
     2401 caactggaag cgctgataag cttcgcccgc accggcgaca ccgtggtggt gcatagcatg
     2461 gatcgcctgg cgcgcaatct cgatgatttg cgccggatcg tgcaaacgct gacacaacgc
     2521 ggcgtgcata tcgaattcgt caaggaacac ctcagtttta ctggcgaaga ctctccgatg
     2581 gcgaacctga tgctctcggt gatgggcgcg ttcgccgagt tcgagcgcgc cctgatccgc
     2641 gagcgtcagc gcgagggtat tgcgctcgcc aagcaacgcg gggcttaccg tggcaggaag
     2701 aaatccctgt cgtctgagcg tattgccgaa ctgcgccaac gtgtcgaggc tggcgagcaa
     2761 aagaccaagc ttgctcgtga attcggaatc agtcgcgaaa ccctgtatca atacttgaga
     2821 acggatcagt aaatatgcca cgtcgttcca tcctgtccgc cgccgagcgg gaaagcctgc
     2881 tggcgttgcc ggactccaag gacgacctga tccgacatta cacattcaac gataccgacc
     2941 tctcgatcat ccgacagcgg cgcgggccag ccaatcggct gggcttcgcg gtgcagctct
     3001 gttacctgcg ctttcccggc gtcatcctgg gcgtcgatga actaccgttc ccgcccttgt
     3061 tgaagctggt cgccgaccag ctcaaggtcg gcgtcgaaag ctggaacgag tacggccagc
     3121 gggagcagac ccggcgcgag cacctgagcg agctgcaaac cgtgttcggt ttccggccct
     3181 tcaccatgag ccattaccgg caggccgtcc agatgctgac cgagctggcg atgcaaaccg
     3241 acaaaggcat cgtgctggcc agcgccttga tcgggcacct gcggcggcag tcggtcattc
     3301 tgcccgccct caacgccgtc gagcgggcga gtgccgaggc gatcacccgt gctaaccggc
     3361 gcatctacga cgccttggcc gaaccactgg cggacgcgca tcgccgccgc ctcgacgatc
     3421 tgctcaagcg ccgggacaac ggcaagacga cctggttggc ttggttgcgc cagtctccgg
     3481 ccaagccaaa ttcgcggcat atgctggaac acatcgaacg cctcaaggca tggcaggcac
     3541 tcgatctgcc taccggcatc gagcggctgg ttcaccagaa ccgcctgctc aagattgccc
     3601 gcgagggcgg ccagatgaca cccgccgacc tggccaaatt cgagccgcaa cggcgctacg
     3661 ccactctcgt ggcgctggcc accgagggca tggccaccgt caccgacgaa atcatcgacc
     3721 tgcacgaccg catcctgggt aagctgttta acgctgccaa gaataagcat cagcagcagt
     3781 tccaggcgtc aggcaaggcc atcaacgcca aggtacgtct gtacgggcgc atcggtcagg
     3841 cgctgatcga cgccaagcaa tcaggccgcg atgcgtttgc cgccatcgag gccgtcatgt
     3901 cctgggattc ctttgccgag agcgtcaccg aggcgcagaa gctcgcgcaa cccgatgact
     3961 tcgatttcct gcatcgcatc ggcgagagct acgccaccct gcgccgctat gcaccggaat
     4021 tccttgccgt gctcaagctg cgggccgcgc ccgccgccaa aaacgtgctt gatgccattg
     4081 aggtgctgcg cggcatgaac accgacaacg cccgcaagct gccagccgat gcaccgaccg
     4141 gcttcatcaa gccgcgctgg cagaaactgg tgatgaccga cgccggcatc gaccggcgct
     4201 actacgaact gtgcgcgctg tccgagttga agaactccct gcgctcgggc gacatctggg
     4261 tgcagggttc acgccagttc aaggacttcg aggactacct ggtaccgccc gagaagttca
     4321 ccagcctcaa gcagtccagc gaattgccgc tggccgtggc caccgactgc gaacaatatc
     4381 tgcatgagcg gctgacgctg ctggaagcac aacttgccac cgtcaaccgc atggcggcag
     4441 ccaacgacct gccggatgcc atcatcaccg agtcgggctt gaagatcacg ccgctggatg
     4501 cggcggtgcc cgacaccgcg caggcgctga tagaccagac agccatggtc ctgccgcacg
     4561 tcaagatcac cgaactgctg ctcgaagtcg atgagtggac gggcttcacc cggcacttca
     4621 cgcacttgaa atcgggcgat ctggccaagg acaagaacct gttgttgacc acgatcctgg
     4681 ccgacgcgat caacctgggc ctgaccaaga tggccgagtc ctgccccggc acgacctacg
     4741 cgaagctcgc ttggctgcaa gcctggcata cccgcgacga aacgtactcg acagcgttgg
     4801 ctgaactggt caacgctcag tttcggcatc cctttgccgg gcactggggc gatggcacca
     4861 catcatcatc ggacggacag aatttccgaa ccgctagcaa ggcaaagagc acggggcaca
     4921 tcaacccaaa atatggcagc agcccaggac ggactttcta cacccacatc tccgaccaat
     4981 acgcgccatt ccacaccaag gtggtcaatg tcggcctgcg cgactcaacc tacgtgctcg
     5041 acggcctgct gtaccacgaa tccgacctgc ggatcgagga gcactacacc gacacggcgg
     5101 gcttcaccga tcacgtcttc gccctgatgc acctcttggg cttccgcttc gcgccgcgca
     5161 tccgcgacct gggcgacacc aagctctaca tcccgaaggg cgatgccgcc tatgacgcgc
     5221 tcaagccgat gatcggcggc acgctcaaca tcaagcacgt ccgcgcccat tgggacgaaa
     5281 tcctgcggct ggccacctcg atcaagcagg gcacggtgac ggcctcgctg atgctcagga
     5341 aactcggcag ctacccgcgc cagaacggct tggccgtcgc gctgcgcgag ttgggccgca
     5401 tcgagcgcac gctgttcatc ctcgactggc tgcaaagcgt cgagctacgc cgccgcgtgc
     5461 atgccgggct gaacaagggc gaggcgcgca atgcgctggc ccgtgccgtg ttcttcaacc
     5521 gccttggtga aatccgtgac cgcagtttcg agcagcagcg ctaccgggcc agcggcctca
     5581 acctggtgac ggcggccatc gtgctgtgga acacggtcta cctggagcgt gcggcgcatg
     5641 cgttgcgcgg caatggtcat gccgtcgatg actcgctatt gcagtacctg tcgccactcg
     5701 gctgggagca catcaacctg accggtgatt acctatggcg cagcagcgcc aagatcggcg
     5761 cggggaagtt caggccgcta cggcctctgc aaccggctta gcgtgcttta ttttccgttt
     5821 tctgagacga cccctattat ggcgaaggta tcattcagga agcgttgatg aaggattatc
     5881 tctgtaaaaa gttattcaac cggctttccg gtaccctggt gatcagagcg cgctgcggca
     5941 ataacattac tggcctggca tgttgcaata ttctttatcc ctcgccccga tacagcggtc
     6001 agctgcatat taaagagctg tatgtttctc agtgcgatcg aaataagggc acgggtaaag
     6061 cgataatgcg ctttatagcc cggcttgcgc ttgaacagga atgccttagc cttagctgga
     6121 acgctgaaaa atccaacccc ggcgctaacc gtttttatca ggctctggga ggcagaataa
     6181 atgatcatat cgtcaattat tacctccacg gggagagcct gagcaaactg gcctcaggca
     6241 tttgagaagc acacggtcac actgcttccg gtagtcaata aaccggtaaa ccagcaatag
     6301 acataagcgg ctatttaacg accctgccct gaaccgacga ccgggtcgaa tttgctttcg
     6361 aatttctgcc attcatccgc ttattatcac ttattcaggc gtagcaacca ggcgtttaag
     6421 ggcaccaata actgccttaa aaaaattacg ccccgccctg ccactcatcg cagtactgtt
     6481 gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa
     6541 tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg
     6601 gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg
     6661 gattggctga gacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt
     6721 caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt
     6781 attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt
     6841 gaacactatc ccatatcacc agctcaccgt ctttcattgc catacggaat tccggatgag
     6901 cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct
     6961 ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag
     7021 caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg
     7081 tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat ctcgataact
     7141 caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttacgt
     7201 gccgatcaac gtctcatttt cgccaaaagt tggcccaggg cttcccggta tcaacaggga
     7261 caccaggatt tatttattct gcgaagtgat cttccgtcac aggtatttat tcggcgcaaa
     7321 gtgcgtc
//
LOCUS       DAZPCS010000172         7254 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZPCS010000172 DAZPCS010000000
VERSION     DAZPCS010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7254
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.174"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..190)
                     /locus_tag="ACHUQ7_003467"
     CDS             complement(<1..190)
                     /locus_tag="ACHUQ7_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFR1136724.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPTCGVVCDPYIC
                     VNSDGISPELTRKYLGEKA"
     gene            710..1405
                     /gene="minC"
                     /locus_tag="ACHUQ7_003468"
     CDS             710..1405
                     /gene="minC"
                     /locus_tag="ACHUQ7_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFR1136725.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALKDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            1429..2241
                     /gene="minD"
                     /locus_tag="ACHUQ7_003469"
     CDS             1429..2241
                     /gene="minD"
                     /locus_tag="ACHUQ7_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFR1136726.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2245..2511
                     /gene="minE"
                     /locus_tag="ACHUQ7_003470"
     CDS             2245..2511
                     /gene="minE"
                     /locus_tag="ACHUQ7_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFR1136727.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2883..3227)
                     /locus_tag="ACHUQ7_003471"
                     /pseudo
     CDS             complement(2883..3227)
                     /locus_tag="ACHUQ7_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3262..3381)
                     /locus_tag="ACHUQ7_003472"
     CDS             complement(3262..3381)
                     /locus_tag="ACHUQ7_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFR1136728.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(3342..3527)
                     /gene="ymgJ"
                     /locus_tag="ACHUQ7_003473"
     CDS             complement(3342..3527)
                     /gene="ymgJ"
                     /locus_tag="ACHUQ7_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgJ"
                     /protein_id="HFR1136729.1"
                     /translation="MSRRYSSATITLVILFFRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3628..3801
                     /gene="ymgI"
                     /locus_tag="ACHUQ7_003474"
     CDS             3628..3801
                     /gene="ymgI"
                     /locus_tag="ACHUQ7_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HFR1136730.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3803..4147
                     /locus_tag="ACHUQ7_003475"
     CDS             3803..4147
                     /locus_tag="ACHUQ7_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFR1136731.1"
                     /translation="MKKKILAFGLISALFCSTPAIADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVSAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            4151..4486
                     /locus_tag="ACHUQ7_003476"
     CDS             4151..4486
                     /locus_tag="ACHUQ7_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFR1136732.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4543..6864)
                     /locus_tag="ACHUQ7_003477"
     CDS             complement(4543..6864)
                     /locus_tag="ACHUQ7_003477"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR1136733.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSACACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKDETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(6981..7190)
                     /locus_tag="ACHUQ7_003478"
     CDS             complement(6981..7190)
                     /locus_tag="ACHUQ7_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1136734.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVVS"
BASE COUNT         1969 a         1723 c         1615 g         1947 t
ORIGIN      
        1 cggctttttc gccgagatat ttccttgtta actctgggga aatgccatct gagttcacac
       61 aaatatacgg gtcgcaaaca acgccgcacg tcggagattg aagtccgcca gcgtcatgtc
      121 cagacgccat tgatgagaac atcatcccca cacctgacaa tccgatataa ataatacgca
      181 tcatattcat agcacatcct gataatccaa ccgtacaaca cttatgctca tttcgtcatc
      241 aatccgtaat caaaaccgct gacctggacg agttacgatg acctgacaga agtatcagac
      301 gtcttctgcg gtttctatga tagagcaatt ctgtggcgga tattaagata acgggtatct
      361 gttctttaat gatgctgcaa aaaatggaaa gttaagaaag cctggcgtgg tgaaaaaata
      421 cacttgcctc aatactggat atttaaacag gtctggagat gctgtcacga ttagggtgcg
      481 gaaaaaggaa gcgtaaataa gaagaatcaa aagtacgacg gcaatgggtt gattgacaag
      541 ggtatttttt aagctatgaa tcagcgccat ttatcacaga atagactttt actctgaata
      601 aatgggaggg tgacttgcct caatataatc cagactataa catgccttat agtcttcgga
      661 acatcatcgc gcgctggcga tgattaatag ctaattgagt aaggccagga tgtcaaacac
      721 tccaatcgag cttaaaggca gtagcttcac tttatctgtg gttcatctgc atgaggcaga
      781 acctaaggtt atccatcagg cgctgaaaga caaaatcgct caggccccca catttttaaa
      841 acatgccccc gttgtactca acgtcagtgc actggaagac ccggtaaact ggtcagcgat
      901 gcataaggcg gtttcggcaa ccggtttgcg ggttattggc gtaagtggct gcaaagatgc
      961 gcaacttaaa gccgaaattg aaaaaatggg gctgcctatc ctgacggaag gaaaagaaaa
     1021 agcgccacgt ccagctcccg caccgcaggc tccagcgcaa aatacaatgc cggtcacaaa
     1081 aacgcgttta atagataccc cggtgcgttc cggtcagcgt atttatgctc cacaatgtga
     1141 tctgattgtt acaagccacg ttagcgctgg ggccgaattg attgccgatg ggaacattca
     1201 tgtctatggc atgatgcgcg gtcgtgcgct ggcaggggca agtggtgacc gggaaacgca
     1261 aatattttgt acgaacctga tggcggaatt ggtgtccatc gcaggtgaat actggctgag
     1321 tgatcaaatc ccagcagaat tttatggcaa agcggcgcga ctgcagttag tcgaaaacgc
     1381 tttgaccgtt caaccgttaa attgatccct ttttaacaag gaatttctat ggcacgcatt
     1441 attgttgtta cttcgggtaa agggggtgtt ggtaagacaa cctccagcgc ggccatcgcc
     1501 actggtttgg cccagaaggg aaagaaaact gtcgtgatag attttgatat cggcctgcgt
     1561 aatctcgacc tgattatggg ttgcgaacgc cgggtcgttt acgatttcgt caacgtcatt
     1621 cagggcgatg caacgctaaa tcaggcgtta attaaagata agcgtactga aaatctctat
     1681 attcttccgg catcgcaaac acgcgataaa gatgccctca cccgtgaagg ggtcgccaaa
     1741 gttcttgatg atctgaaagc gatggatttt gaatttatcg tttgtgactc cccggcaggg
     1801 attgaaaccg gtgcgttaat ggcactctat tttgcagacg aagccattat taccaccaac
     1861 ccggaagtct cctcagtacg cgactctgac cgtattttag gcattctggc gtcaaaatca
     1921 cgccgcgcag aaaatggcga agagcctatt aaagagcacc tgctgttaac gcgctataac
     1981 ccaggccgcg taagcagagg tgacatgctg agcatggaag atgtgctgga gatcctgcgc
     2041 atcaaactcg tcggcgtgat cccagaggat caatcagtat tgcgcgcctc taaccagggt
     2101 gaaccggtca ttctcgacat taacgccgat gcgggtaaag cctacgcaga taccgtagaa
     2161 cgtctgttgg gagaagaacg tcctttccgc ttcattgaag aagagaagaa aggcttcctc
     2221 aaacgcttgt tcggaggata agttatggca ttactcgatt tctttctctc gcggaagaag
     2281 aacacagcca acattgcaaa agaacggctg cagattattg ttgctgaacg ccgtcgcagc
     2341 gatgcagaac cgcattatct gccgcagttg cgtaaagata ttcttgaggt catttgtaaa
     2401 tacgtacaaa ttgatcctga gatggtaacc gtacagcttg agcaaaaaga tggcgatatt
     2461 tctattcttg agctgaacgt gaccttaccg gaagcagaag agctgaaata agcccgctgt
     2521 aaaaacgcat ttatcttcaa ggcagagtca tttctgcctt gagtttttca tccctctcat
     2581 ccacgttgtg gtaaagcggc gagtattctt gctgatactc ctcattgcta tttccacctc
     2641 cccctttaca ccttaaggct gttcccacgc acagaacaaa atgtttatgc ctggttgagt
     2701 aaataaccat gttattagtg tgtatatact gggagatgat aaccccgcaa tttcattatt
     2761 cattgttctc ccataggatg aggtttcccg ccgtaactgg cgggcttttt ttgccccaga
     2821 attttctctt tcaacatcct gtaaacgaaa actgcgccga agcgcagttt aatcgttgat
     2881 ggtcagaacg accagttcac accagccacc gcgttccacg gggactccac accggcacca
     2941 tggctatacc ccaccccgaa atgcccgctt aacgtactgc tgaatgaggc tttaatacct
     3001 gcctggtata ttccacgtct gcccgacaaa tcattgacaa aattaccgtc actattcact
     3061 ttcacccgat tatcatcgac aaattctttg cgcacagccg ccttcaggca gggttcaatt
     3121 tccataccgt tccccagacg catgttgtaa ctcagcgttg cacccagttc acgatatata
     3181 ctgcgggtat cgactgattt cgattccatg ccattggata aatgatagta gcgtctaagt
     3241 gacctaagct ctgctgcaac atcagcccgc aggcaccaga ccaggggatt catcctgaag
     3301 agacagcgca ggtgtattgt gttcaccgct caccaaagac atcatgatga aatgatgata
     3361 tcccgcataa gaaagaggca ttttttacac gcagtgcgct gaagtgtagt tggataaaaa
     3421 atcactcttt tcagataata ctgtcgtacg cttatcttta gacaatgagg ccgtcggaaa
     3481 aataaaatga ctaatgtaat cgttgctgat gaatatcttc tgctcacata aaaatcacac
     3541 aataatattg ataaatcaga ttatcttacc tttacaacat tgcgttaaca gtcaatgaca
     3601 cttcttgaat aaaaatattg tcaatcaatg agttattcct catttaagat tattctcatt
     3661 cacgtaaaaa acataattcc tatcatcaca gccacgctca ttttgaatta tctgaataat
     3721 agcgagcgtt ctttagttaa gcagatctta attgaagacg aaattattgt gtgtgcaact
     3781 tacttaatac cggacatcta agttgaagaa aaaaatactt gccttcgggc ttatctcagc
     3841 attgttctgc tccacgccag cgatagcgga tatgaaccgc accacgaaag gtgcattgct
     3901 aggcgcaggc gttggtttac ttactggcaa tggtgttaat ggcgtactga aaggtgcagc
     3961 tgttggcgct ggtgttagtg cagtaacaga aaaaggccgc gacggtaaaa atgcgcgtaa
     4021 aggtgcaaaa gtgggtgccg ccgttggtgc tgtcactggc gttctgaccg gcaatggcct
     4081 cgaaggggcg attaaaggtg ccgtgattgg cggtacgggt ggtgcaattc tgggtaaaat
     4141 gaaataagga atgatgatga aaaaatttgc tttactggcg ggcctgtttg ttttcgcacc
     4201 aatgacctgg gcgcaggact acaatattaa aaacggtttg ccgtcagaaa cctatattac
     4261 ttgcgcagaa gccaacgaaa tggcaaaaac tgacagcgca caggttgcag aaattgttgc
     4321 ggtaatgggt aatgccagcg ttgccagccg tgatttaaaa attgagcaat ctccggaact
     4381 gagcgccaaa gtggtagaaa aactgaatca ggtttgcgct aaagacccgc aaatgctgtt
     4441 gatcacggca attgatgaca ccatgcgtgc tatcggcaaa aaataatatt tcatatgtac
     4501 cgcgtcgatg aatacgacgc ggttttttag ctaacggcgg gatcagaact tatatttcag
     4561 gcccaccatc actgcggtgt cattgtagcc attatcaccc agctgcacgc ccacattccc
     4621 ccagacgctg gccgctggat tcaggttgcc atttacgcca agtttaattt cccccaaatt
     4681 actcacccca tcttgttcga ctttcacacc gttcattgag acagcaaaat ctttactgtt
     4741 atggatccag ttcatttcaa caaaaggttc aaagtcgtca ccgtgcggac cgctgttttt
     4801 ctcctgagta cgaataaagg tgcggaagcc gagtcgggtc tggatgttgt tatttgcgtc
     4861 attctcaata cgtgagccat tggcttccgt gtgcgcatta tgatcgacgc ccatccagat
     4921 agcctgtgcc tgtggctgga agatccacgt atatttcgcc gcttccagac caaaggattt
     4981 attcagggca aaaccgtaac ccgcttccag cgagacggtt gcacctttcg cgctatagga
     5041 ttcataggct aattcatccc ctttcacgga gtttttaaac cagctatatt gcgcccagga
     5101 gtcaatgtat gcgccttttt tactgatgtc atcggcaaac caagtggcat acagccctgc
     5161 gctatagcct ctgacactcc ctttcgaacg ataatccgac acgctggaat gagttaagtt
     5221 gtagtcgcgg gcataaccag ccatcactcc caaacgccaa ctgtcactat cggtaaaacc
     5281 accggttaac agatcgcccc ctaactgcga gacgtagcga tgcgaggtcg ttctcaactg
     5341 tccgttgctg tcacgccagg cattatgccc gccaatttga cgtagccaaa gtcggcttga
     5401 acgctcctgt tcagttacag gatcgatgta gcgcgtttca cccgcacggt cgttcagacg
     5461 cataacaaaa agagtgttag cggctgccag gttggcgacg taacttccag cttcggggcg
     5521 caggacgggg gtaggcgcaa cagtaggcgc tgaacttcct ccggagtttg tttcaccgcc
     5581 gctcgttgcc tggaagttag tcagatacca gtttttattg gtcgcttcgg tattacctcg
     5641 ggttaatgaa tattcgtagg caccggcgaa aatcctcgaa tccttaataa attccccatt
     5701 tgattccccc tcaacgctga taatctctat cccatttaac gtgtaagctc cgctgcctcc
     5761 tgcattggta actttaactc gggttgttcc agaggtattt ccattaataa cgactttatc
     5821 cgtagctgaa ttatcatcat ccaattcagt tctgagatgc aatacgccat tattaccaac
     5881 gtaattttca gttattgtta atcttgttgg ttcgacatcc cgtccatctg cccgggaaat
     5941 atagaccgta ctattatcaa ccgtgaggtt gctcaccgtt gagtcagctt taatatccca
     6001 ggtactatta tctgaaagcg acagataggt atgagtgtta tcatctgttg aaatattagc
     6061 tgaacccgtt atttttgaat tagttgcatc gacggtcaaa tgacctttaa tattattgat
     6121 aaaaacatct gcagccgatg atacctcact atccgtaatg gtcacgttac tcgtctcatc
     6181 tttatggtag aggatttcat ttgctgaccg tagtgagcta ccagataaaa tatctaccgt
     6241 tgcttcctga ccatttatgg aagactccag cacaattcca gacccctgat gcgcgactgc
     6301 agatgtattt ttcagcgtca ccgtgccgcc attataaatt ttagcaatcg cggcgtcagg
     6361 acccgttgta taaatttctc cctggttaaa tgaaactgtc ccctggcggg cagcgatggc
     6421 atgagcattg tccccatgcg tggtgatatc aacatcggtt aatgtaacgc tggcattggt
     6481 ataatcaagc gtgtcaccag ccttgacgac agtgccatta gcgtacgcgg cataactttc
     6541 tgttccgtgg gttgtgatct ccccttttgc catatccagt tttgctatgg cattattttc
     6601 attacttcct gtatcggcag tactttcttc atcaagagaa gatacacacg cacacgcgct
     6661 tccgggagat gcacacgcaa gaacgccgac tgcgtgatta cccgcagtaa caataaatgt
     6721 atcatctccg tcagaaaaag agggggtaac cgaaccgtca gagttgactc ttattatggt
     6781 gtcagtattc aggtttccac tcgtttgact tagcaaacca attgagtaat cccccgttgt
     6841 gacaatcgtg tttttaccct gcaaaataac agcaccgcca aattcactta cgattgcgct
     6901 agctgttaat gctgggctta gaggactatt gtcagaagga tataaaccgc ccgttgttac
     6961 aatgacgcca ttgttaatgg ttaacttact accctctcct gcaacggata cccgtaactt
     7021 ccattacccg ttgttataac tagcgcatta tcaatatctt ttacttttcc atcttttatt
     7081 gccacagcat gtttatattc atcgtgctga ataaactgtt cagcatatgc attacccacc
     7141 gccagagcaa taagtcccaa actaaacccg gggagttttt tcagtttcat agattaacta
     7201 tccattcatt ggattaacaa acattcctgg tcaaataacc tatgagctca gatt
//
LOCUS       DAZPCS010000173         7188 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZPCS010000173 DAZPCS010000000
VERSION     DAZPCS010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7188
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.175"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            28..879
                     /gene="hchA"
                     /locus_tag="ACHUQ7_003479"
     CDS             28..879
                     /gene="hchA"
                     /locus_tag="ACHUQ7_003479"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFR1136735.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHSDNPLNGYSICAFPDAADKQTP
                     DIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKRLTGDSPFAANALGKLAAQE
                     MLAAYAS"
     gene            complement(987..2345)
                     /gene="hprS"
                     /locus_tag="ACHUQ7_003480"
     CDS             complement(987..2345)
                     /gene="hprS"
                     /locus_tag="ACHUQ7_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFR1136736.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQIRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVKCNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDANGSLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLSKHNVFRITLPQ
                     RN"
     gene            complement(2345..3016)
                     /gene="hprR"
                     /locus_tag="ACHUQ7_003481"
     CDS             complement(2345..3016)
                     /gene="hprR"
                     /locus_tag="ACHUQ7_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFR1136737.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            3149..3562
                     /gene="hiuH"
                     /locus_tag="ACHUQ7_003482"
     CDS             3149..3562
                     /gene="hiuH"
                     /locus_tag="ACHUQ7_003482"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFR1136738.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            3671..4675
                     /gene="msrP"
                     /locus_tag="ACHUQ7_003483"
                     /pseudo
     CDS             3671..4675
                     /gene="msrP"
                     /locus_tag="ACHUQ7_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
     gene            4676..5311
                     /gene="msrQ"
                     /locus_tag="ACHUQ7_003484"
     CDS             4676..5311
                     /gene="msrQ"
                     /locus_tag="ACHUQ7_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFR1136739.1"
                     /translation="MRLTAKQVIWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
     gene            5569..6219
                     /gene="zinT"
                     /locus_tag="ACHUQ7_003485"
     CDS             5569..6219
                     /gene="zinT"
                     /locus_tag="ACHUQ7_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HFR1136740.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
BASE COUNT         2032 a         1629 c         1507 g         2020 t
ORIGIN      
        1 taactaagca acaataagga atacactatg actgttcaaa caagtaaaaa cccgcaggtc
       61 gatattgctg aagataatgc gttcttccct tcagaatatt cgcttagcca atatacgagt
      121 cccgtctctg atcttgatgg cgtggactat ccaaaaccgt atcgcggtaa acataaaatt
      181 ctggtgatcg ccgcggacga acgttatttg ccgaccgata acggaaaact gttctcgacc
      241 ggtaaccatc cgattgaaac gttgctgccg ttgtatcatc tccatgctgc aggtttcgaa
      301 ttcgaagtgg cgaccatttc cggtctgatg accaagtttg aatactgggc tatgccgcac
      361 aaagatgaaa aagtaatgcc attctttgaa cagcataaat cgttattccg taatccgaag
      421 aaactcgcgg atgttgtcgc cagcctcaac gctgatagcg aatatgcagc aatctttgtt
      481 cctggtgggc atggcgcact tattggttta ccagaaagcc aggacgtggc tgccgcttta
      541 cagtgggcaa tcaaaaatga ccgttttgtt atctcccttt gccacggtcc ggcggctttt
      601 ctggcacttc gccacagcga taacccactg aatggttatt ctatttgcgc attcccagac
      661 gccgcagaca aacaaacgcc agatattggc tatatgccgg gtcatctcac ctggtacttc
      721 ggcgaagagt tgaagaaaat gggcatgaac atcattaatg acgacatcac cgggcgagta
      781 cataaggacc gtaaacgtct caccggcgac agtccttttg cagcgaatgc gttgggtaaa
      841 ctggcggcgc aggaaatgct ggcagcttac gcgagttaat ccctgttaca ttgtgatgta
      901 agcgcataat caatcgcagc cacgttaata ctcacgacgt ggctgcgttt tttcacggtt
      961 aacttatggc gtactgaagc ccgatgttaa tttctttgcg gtaacgtaat tcggaacacg
     1021 ttatgcttac tgagatagtg atacgtagca cttcctccat gtaattcggc aatcgctttg
     1081 actaaagaaa ggccaagtcc ctgacctacg gaatgacgcg aattatctcc ccgccaaaat
     1141 ctacggaaga gtttttcagg ctcattaatt ttcgttccag gactggcgat atcaatatta
     1201 agagagccgt tggcatcaag aaaactggtt atatgaatac gcgatttttc tggtgaatat
     1261 cgaatggcgt taacaataag attcgataac attcgttgca gtaaaatttt atccgcaaag
     1321 atttgttgat tgcacttgac cttaaagcaa atctctttct cgtctgaaag atattcaaga
     1381 tagtccaaaa gattttcaac ttccttattg agagaaagcg aatccagttt caccaaaaca
     1441 ttgtttttgt ctgccctggc aagaaacagt atattctctg ttaaccgcga aatattttcc
     1501 agctcttcaa tgtttccggc aattgttttt tgatattcag cgatacttct gatttgactg
     1561 agcgtaacct gattctgacc cagtaatgca ttaattggcg ttctaagttc atgagcgaga
     1621 tcgtcagcaa attgactaag gcgctcaaaa tctttgacta aagcatgatg cattttattc
     1681 aacgcctgcc caagaggttt tagttctctc ggtaatgcgt taacctcaac cggcactcgg
     1741 ctatcgttat aattcagcgc ttccgttacg ccactcaact ttttgatctc tcgcaacccc
     1801 gtcctgatta acaacggact taatactgag cacagtataa tggcgacaat gcaaattata
     1861 atgctattaa ttttatactg ttcaagcata ttatgtctgg ctgaagccaa tttagccaca
     1921 gtaaccgtta atgatggcga aacttcatca atgttaattc gtaaagcatc tatctctgta
     1981 tcattaataa tgcttctgta aatgcctgct gcgctgattg tctcactagc aggtatgtta
     2041 tttaacatgt catcactgac atttgtccgg ttaacaattt tattgatgcc atcactatga
     2101 atgatcaaaa tatcctgact aacatccatc atccggttaa agtacacagg taacgtatct
     2161 ggatttaccc catcaattaa caactgcttg atctgcgctg tccggttaat gagtgttgta
     2221 tcatcgcgcc atttcaactc acttgccagg ccattataga gagtccatac aattccagcg
     2281 ccagcaacag actgcagaaa tataaaaagc aaggttaaac ggacggttat agataatctt
     2341 ttcattattt ttttaccgct acgaatgaat agcccatccc ccggattgtg gcgattagct
     2401 tttcaggaaa aggatcatca acttttgcgc ggagcctgcg aatggcgaca tccaccgtat
     2461 tggtatcact atcaaagttg attccccaaa tttcactcgc aataaccgtt ctgggtataa
     2521 tctcgccagc tctggaggcc agtagccaaa gtaactgaaa ctccttgcgc gtcagtgtaa
     2581 tactgatatt gtccctgctg acactttgac tgacagagtc catttttaag ccactgattt
     2641 ctaatgttga attcagagcg tgatgttgcc ttaattgtgc ccgaaccctt gctagcaact
     2701 cagaaaatga aaaaggtttt accagataat catttgcccc actgtccaac cctctgactc
     2761 tgtcatcgac agaatccctt gcagtaaggc aaataacagg ggtttgcttt gccgttctta
     2821 acgtttgtaa gatctgccag ccatccatac ccggaagcat aatatccaga atgatcaatg
     2881 cataatcatc cttcagcgca agataaagcc catctctgcc atcagaaacg gcatcaatga
     2941 cataacccgc ttcggaaagc ccctgcgtta cccattcctg ggtcctttga ttatcttcaa
     3001 taagtagaat cttcatgaaa ataatatgcc ataaatcata tgttatagaa acaacctggt
     3061 tcattacaaa attgtaatgc tgctgtaagg ttaccctgga cgttttttcg ctatcctcaa
     3121 aactcattca catgacaagg atataaacat gttaaagcgt tatttagtac tctcggtagc
     3181 aacggcagcc ttttcattac cttctttggt ttatgccgca caacaaaaca ttcttagcgt
     3241 gcacattttg aaccagcaaa caggaaaacc cgctgccgac gtgacagtca ctcttgaaaa
     3301 gaaagcggat aacggctggt tacaacttaa taccgccaaa acagataagg atggacgaat
     3361 taaggcactg tggcccgagc aaactgcgac tactggcgat taccgtgtcg tatttaaaac
     3421 cggggactat ttcaagaaac aaaatcttga aagtttcttc cctgagattc ccgttgaatt
     3481 tcatattaat aaagtgaacg agcattatca tgtgcctttg ttactcagcc aatatgggta
     3541 ttcaacctat cgtggcagtt aatgcgtaaa tatgtaaaag ctgatctctg ctactgttag
     3601 ggaggtgact ccctgacaga ttaacagtaa acagctcttg ttggttaacg acaaaaaagt
     3661 gtgatggctt atgaaaaaga agcaattttt aaaagaatca gatgttacgg ccgagtcggt
     3721 attctttatg aagcgtcggc aggtgttaaa agcactgggc atcagcgcag ctgcattttc
     3781 tttgcctcac gctgcgcatg ccgatctgct tagctggttt aaagggaacg atcgtccgcc
     3841 cgcccccgcc ggaaaaccgc tggagttcag caagcctgcc gcctagcaaa ataacctgcc
     3901 actgacgcca gcagataaag tctccggtta taacaacttc tatgaattcg ggctggataa
     3961 agacgatccc gcggctaatg ctggtagcct gaaaaccgat ccatggacac tgaaaatcag
     4021 cggcgaagtg gcaaaaccat tgaccctcga tcatgatgat ttaacccgtc gcttcccgct
     4081 ggaagagcgt atttatcgta tgcgctgcgt ggaagcgtgg tcgatggtgg tgccgtggat
     4141 tggttttccg ctacacaaat tgctggcgct tgccgaaccc accagcaatg cgaagtatgt
     4201 cgctttcgaa acgatttatg cgccggaaca gatgcctggc cagcaggacc gctttatcgg
     4261 cggcgggctg aaatatcctt atgtcgaagg attgcgtctc gacgaagcaa tgcatccgct
     4321 cacactgatg accgtaggtg tttatggcaa ggcgttacca ccgcaaaatg gcgcgccggt
     4381 gcgactgatt gtgccgtgga aatatggctt taaagggatt aaatcgatcg tcagtattaa
     4441 gctgacccgc gagcgtccgc caaccacctg gaatctggca gcgcctgacg aatacggttt
     4501 ttacgccaac gttaatccgc atgttgatca cccgcgctgg tcacaggcta ccgaacgatt
     4561 tattggttca ggcggcattc tcgatgttca gcgccagcca acactactgt ttaatggtta
     4621 cgccgaccag gtggcatcgc tgtatcgtgg cctggatttg cgggagaatt tctaaatgcg
     4681 tctgacagca aaacaagtga tatggctgaa agtttgcctg catcttgccg gattgttgcc
     4741 gtttctctgg ctggtctggg cgatcaatca cggtggactg ggtgccgatc cggtgaaaga
     4801 tattcagcat tttaccggtc gcactgcact gaaatttttg cttgcggcct tgttaatcac
     4861 acctctggca cgctacgcaa aacagccgtt attgatacgc actcgccgcc tgttaggctt
     4921 atggtgcttt gcctgggcga cactgcattt aaccagttac gcattgctgg agttgggcgt
     4981 gaataatctg gcgttattag gtaaggagtt aattacccgg ccttatttaa cgttaggaat
     5041 tatcagctgg gttattttgc ttgctttagc gttcacttca acccagtcga tgcagcgaaa
     5101 actcggcaaa cactggcaac agttgcataa cttcgtctat cttgtcgcga tcctggcccc
     5161 aatacattat ctgtggtcag tgaagattat ttcaccgcag ccactcatct acgccgggct
     5221 ggctgtactg cttttagcct tacggtataa gaagttgctt tcccttttca accggttacg
     5281 caagcaggcc cacaacaaat tatctctata acttcatatt gccgacaaac ttcgcgttat
     5341 cccggccgga tgcggcgcga gcgccctatc cggcccacac agatcgcatc aagttatgtt
     5401 tcgtttgtca tcaacctttc atcaccagaa taatttattt tatttcaata agatagttca
     5461 atacacctga gaataccatg ctctcatttc ctaatgagtt gttgcatttt ttcatatgtt
     5521 acaatataac attacacata atgtacatta actctggagg aaattgtttt ggcgattcgt
     5581 cttcataaac tggctgttgc tttaggtgtc tttattgtta gcgctcctgc cttttcgcat
     5641 ggtcatcatt ctcatggcaa acccttaaca gaggtcgaac aaaaagctgc taatggtgtt
     5701 tttgatgata ccaatgtaca aaaccgaacg ctcagtgact gggatggagt ctggcaatcc
     5761 gtttaccctt tactgcaaag tggcaaactt gatcccgtct ttcagaagaa agcggatgca
     5821 gataaaacca aaacatttgc tgaaattaaa gattattatc gcaaaggtta tccgacagat
     5881 atcgaaatga ttggcattga ggacggcatt gtcgaattcc atagaaataa tgaaacaaca
     5941 tcctgtaaat atgattacga tggatacaaa atactcacct ataaatcagg caagaaaggc
     6001 gttcgttact tattcgaatg taaagatcct gaaagcaaag cccctaaata tatacaattt
     6061 agcgatcata taattgcacc acgaaaatca tctcattttc acatttttat gggtaatgat
     6121 tcccagcaat ctttattgaa tgaaatggaa aactggccaa cgtattatcc ctatcagttg
     6181 agtagcgaag aagtggtcga ggagatgatg tctcattgat ttttttgcgc tgacttcagt
     6241 tatttaacag aacagtgcct ggtagtaaca acaataattt accaagacaa ataagtaaga
     6301 caaactcaga cacgctcata cttgagtcgt gtctttttta ttatgtctgt agaaaaaaac
     6361 tgagaaaaag gctaacaata aatacacaac tcagccatac agactggcag cgcaaatgtt
     6421 tccaggagca agtcatctgt catggtaaag ctaaccagaa atataggtta attttggtgt
     6481 ttaaaccttt tttacttgtt agtatgatac aaaggctttc aaaaaagctg ggaaattcaa
     6541 caactcttgg aaattatgga ctttgtcccg cggctttaat ctgacggccg cgttcctttt
     6601 ttggctcaat tattgacccc ttctctacgc aacttcagtt cccgccagca actttgcggc
     6661 agtttcatag gatcaatgtc taaaagaata atggtgaccg ataagaaaac gactgaataa
     6721 ctgtagattt tcgctcgaaa ccttcctgtc agctccatag cgaatcaagc gctgaatgtc
     6781 acagtatcga acagaaaaca gtgacgatct aacccttcaa gaatattcta cgattgttct
     6841 gtttaggaaa agcaaggcgg gaagtcggga gataagtcat tgataaaatg gcggagagag
     6901 ggggatttga acccccggta gagttgcccc tactccggtt ttcgagaccg atgatacaga
     6961 gtcgaaaaat caaagagtta aaaatttttc ttggaaaaaa tgtcttaaaa tcgtacacga
     7021 aaaatcaatg aattacataa cattaaagaa gaatattcca agccttcttg agtccaccac
     7081 ctgttttcag gtgtcattca gacgtgattt gccccagatt gggaaagcac gcttttttct
     7141 cagcttattc cgttttgcct gtgtgttgaa taattccact gcagttat
//
LOCUS       DAZPCS010000174         7183 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZPCS010000174 DAZPCS010000000
VERSION     DAZPCS010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.176"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(371..1198)
                     /locus_tag="ACHUQ7_003486"
                     /pseudo
     CDS             complement(371..1198)
                     /locus_tag="ACHUQ7_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
     gene            complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="ACHUQ7_003487"
     CDS             complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="ACHUQ7_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HFR1136741.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            2479..4014
                     /gene="lsrA"
                     /locus_tag="ACHUQ7_003488"
     CDS             2479..4014
                     /gene="lsrA"
                     /locus_tag="ACHUQ7_003488"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HFR1136742.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAVGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGSNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLFISSDMEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4008..5036
                     /gene="lsrC"
                     /locus_tag="ACHUQ7_003489"
     CDS             4008..5036
                     /gene="lsrC"
                     /locus_tag="ACHUQ7_003489"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HFR1136743.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGALVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGIS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFILNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5036..6028
                     /gene="lsrD"
                     /locus_tag="ACHUQ7_003490"
     CDS             5036..6028
                     /gene="lsrD"
                     /locus_tag="ACHUQ7_003490"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HFR1136744.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6040..7063
                     /gene="lsrB"
                     /locus_tag="ACHUQ7_003491"
                     /pseudo
     CDS             6040..7063
                     /gene="lsrB"
                     /locus_tag="ACHUQ7_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
     gene            7090..>7183
                     /locus_tag="ACHUQ7_003492"
     CDS             7090..>7183
                     /locus_tag="ACHUQ7_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="involved in autoinducer 2 transport and processing;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase LsrF"
                     /protein_id="HFR1136745.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGA"
BASE COUNT         1676 a         1812 c         1876 g         1819 t
ORIGIN      
        1 tttcgtatgc agtgtctatg cgacagacac tgcgctctta gcatggggca gggaataacc
       61 aggctatttc tccatgcaca ggccaattat gaaagaggag aggcttaagt tcttgaaccc
      121 tgaacgaata acgactacca gaaaagcact tcgcatcact cagacatgat ttaacataat
      181 atacattatg cgtagtaata ttgtagtagc taaattgaga tgtccgtctc tgactaaata
      241 gacatgtccg cgctacagtc tcaccacagc gatcactgct ttcaaaggat gggactcatg
      301 acggcatacg gaacggagta atcggcaact gatgccgggc ctcgctgagc gtgcactgct
      361 tattatccgt gaacgtgccg ccaagaaccg cggtttgtgc cggacgcaca acgcctaacc
      421 caaggcaacc aagctgcacg tcgcctcctc caacgaccac cagagtgccc gccttcagac
      481 cgcagagttc cgccgcttgt gaacttacca cgcccagcaa tgtgccggtt tctttgacag
      541 gagaaagaat atcggcacgc aggccagcca tatccagcaa tgcaggtttc caggcacggg
      601 tggttagatc aagaagtccc gtggtgccag cgttagaggg atccaccgcc agttcgccgc
      661 tgagcatata ggccagccag tcgctgatca tggtgatggt tgatgcctga cggtaaatat
      721 cggaacgatg gtgcgccagc caaagtaatc tggggatggc acttaaagcc agtgtttgtc
      781 cggtcgctcg ataaacttcg ttttcaaagg tattgttgtg cagttcttta agttcgctaa
      841 cttcgcgtgc cgctctggca tccacattgg cgcaggccca gatcggtgtt ccttcattgt
      901 tatataaaac aatgccttca cgcatagaac atgccgaaac ggcagcgata tactccgggt
      961 ctatgccggc gttgtgcagc gcctggcgca tacactcaca cgccagttgc cagtttttgt
     1021 tgagatcaaa ttccatagaa ccaggaacgt ccggtactgc cagatgccgc cactccgcct
     1081 gtcccactgc tatttgattg ccttccaggt cgaatatcac agcccgaata cttccggtgc
     1141 ctgcatccag cgccatcagg tagtactttg attctgaagg ggtaaagagt cgagccatta
     1201 gattatcctc ggcttatgcg ttctgtctgt tcctctatac gttctccatc attcccggta
     1261 ataaggtcat gcaaatttaa ctacgtaaaa tcgccgctgc tgtgtcctga tcggtaacca
     1321 gtgcgttgat ataaccgcct ttcattgcag cggcaattgc ttcagcttta ttttctcccc
     1381 ctgccacgcc aacccggacg ggtatggtct tcagcgcgct taaaggtaag ccaatcagtt
     1441 cgttatgtat tttgatatcc gtgacaacgt caccttttgc atcaaaaaag tagcctaaaa
     1501 tgtcgccaac cgcccctttt cggccaatca ttaactgttc gccctggctg atataaccgg
     1561 agcgaatgat tgtcgcgtcg tcttgttgac tcacggctcc aatgccgaca atcgccacat
     1621 ccgctgcttg cgcggctagc agaacatctt tgacgcaatt ttcatttttt agcgtacggg
     1681 caatgtcagc ggaggatgcc cgcaacggag ccggaataat attcacactg cacgccgcgt
     1741 taagctgccc gatacccgtc atataagaac cgacgccacc ggagagcgtg accaggcgaa
     1801 tttgctgtga cgaaataaaa ccacttaagc gttgcagcgt attcatggtt gcctcgccaa
     1861 aaccaatcgc cagcatctgt tgtggttgaa gtaaactcat caacatatgc gccgcgccta
     1921 tccctagtcg cccgccgaca tcagcatccg caagcccagg gatcacccgg acatgttgca
     1981 gcgaaaactg acgacgtaat tgagtttcat attccagaca gccttcaaag cgagaattaa
     2041 tctgtacgcg aataatgccg gactgatgcc ctttctccag caagcgcgac actttcaaac
     2101 gggtcaggcc gagacgatcg ctgatctcgc tctgggtcag cccgtcgtga tagtaaaacc
     2161 acgcgatccg cgcgacctgt tcttcttcac acattccctg ttctgaaatt gccgaatcgt
     2221 tgattgtcat aattcattct tcactttgaa catatttaaa tctttaatgc aattgttcag
     2281 ttcttgcgca tttatatctg tgatggcaac cacagtttga ctctacgagc atgaacaaac
     2341 gcaaccgtga aaatcaaaat agcataaatt gtgatctatt cgtcggaaat atgtgcaatg
     2401 tccacctaag gttatgaaca aattaaaagt agaaatacat ttgttcaaaa ctcacctgca
     2461 aaactgaacg ggggaaatat gcaaacgagt gatacccgcg cgttaccgct actttgcgcc
     2521 cgctcggttt ataaacagta ttcaggggtc aatgtcctga aaggcatcga ttttacgttg
     2581 catcaggggg aggtccacgc cctgctcggc ggcaatggtg ccggtaaatc gacgttaatg
     2641 aagattattg ccggtattac ccctgctgat agcggtacgc tggagattgg gggcaacaac
     2701 tacgccagat taacgccagt tcatgctcat cagctgggca tttatctcgt tccccaggaa
     2761 ccgctgcttt tcccaagcct gtcgataaaa gaaaacatcc tgtttgggct ggcaaaaaaa
     2821 cagctctcca tgcagaaaat gaagaacttg ctggcggcgc tgggctgcca gtttgatctg
     2881 catagtctgg caggatcgct ggatgtcgcc gatcgccaaa tggtggaaat cctccgcggg
     2941 ctgatgcgcg actcgcggat tctgatcctc gatgaaccta ccgcctcgct tacccctgcg
     3001 gaaaccgaac gcttgtttag tcgcttgcaa gagctgcttg ctactggcgt gggtattgtt
     3061 tttatctcgc ataagctgcc ggaaattcgc cagattgccg atcgaattag cgtgatgcgc
     3121 gacggaacca tcgccttaag cggcaaaacc agcgaactgt ctaccgacga cattattcag
     3181 gccatcaccc cagcggtacg ggaaaaatcg ctctctgcca gccaaaaatt atggctggag
     3241 ttacctggta accgcccaca acatgccgtc ggaacgccgg tgctgacact ggaaaatctg
     3301 actggcgaag gcttcaggaa tgtcagcctg acgctcaatg ccggagaaat tctgggcctg
     3361 gctgggctgg tgggagccgg acgcacagaa ctggccgaga cgctctatgg tctgcgtact
     3421 ttgcgtggcg gacgcattat gctgaatggt aaagagatca ataaattatc cactggagaa
     3481 cgtttactgc gcggtctggt ttatctgccg gaagatcgcc agtcatccgg actgaatctc
     3541 gatgcttcgc tggcctggaa cgtctgcgcc cttactcata accttcgtgg attctgggcg
     3601 aaaaccgcga aagataatgc caccctggaa cgttatcgtc gggcgctgaa tattaaattt
     3661 aaccaaccgg aacaagctgc acggacatta tccggtagca accagcaaaa aatcctcatt
     3721 gccaaatgcc tggaagcttc gccgcaagca ttgattgtcg atgagccgac gcgcggcgtg
     3781 gatgtctcgg cccgtaatga tatctaccag ctgttgcgca gcatcgccgc acagaatgtg
     3841 gctgtgctgt ttatctcctc tgatatggaa gagatcgaac tgatggcaga tcgtgtgtat
     3901 gtgatgcatc agggcgaaat tacccactct gcactgaccg ggcgcgatat taatgtcgag
     3961 actattatgc gcgttgcctt cggcgatagt cagcgtcagg aggcatcatg ctgaagttta
     4021 ttcagaacaa ccgtgaaatc acggcattgc tggcggtggt gctgctgttt gtattacccg
     4081 gttttctcga ccgccagtat ttaagtgtgc aaacgctgac catggtttat agcagcgcgc
     4141 aaatcctgat cctgctggca atgggcgcga cgctggtaat gcttacgcgc aatattgatg
     4201 tttcagtggg ttcgattacc ggaatgtgcg cggtgctgtt ggggatgtta ctgaacgcag
     4261 gatattcact acctgttgcc tgtgtcgcga ctttactgct tggtttgctc gcgggatttt
     4321 tcaacggtgc cctggtcgcg tggctaaaga tccctgccat tgttgccacc cttggcacgt
     4381 tagggttgta cagaggcatc atgttgctgt ggactggcgg taaatggatt gaagggttac
     4441 ccgccgaact gaaacagctc tccgccccgc tgctgcttgg catttcagca attggttggt
     4501 tgacgataat tctggtggca tttatggcct ggctgctggc gaagacggcg tttggacgca
     4561 gtttttatgc cacgggcgat aatttacagg gcgctcgtca actgggcgtt cgtacggaag
     4621 ccattcgcat tgtggcattt tcgttgaacg gctgcatggc ggcactggcg ggaattgtgt
     4681 ttgcttcgca gattggtttt atcctcaacc agaccggtac cgggctggag atgaaagcaa
     4741 ttgcagcctg cgtgctgggc ggcattagtt tgctcggtgg ttccggtgcg atcatcggtg
     4801 cggtactcgg cgcatggttc ctgacgcaga tcgatagcgt actggtgctg ttgcgcattc
     4861 cggcatggtg gaatgatttt atcgcgggtc tggttctgct ggcggtgctg gtgtttgatg
     4921 gacgcctgcg ttgtgcgctg gaacgtaatc tacggcggca aaaatatgcc cgctttatga
     4981 cgccaccgcc atccgttaaa cccgcttcgt caggtaaaaa acgggaggcc gcataatgcg
     5041 tattcgctac ggttgggaac tggctcttgc cgcactgctc gttattgaga ttgtcgcatt
     5101 tggtgcaatt aacccgcgaa tgttagatct caatatgttg ctgttcagca ccagtgactt
     5161 tatctgcatt ggcattgtcg ctctaccgct gacgatggtg attgtcagtg gcgggatcga
     5221 tatttcgttt ggttcgacca tcggcctctg cgccattgca ttgggcgtac tgtttcaaag
     5281 tggtgtgccg atgccgctgg cgatactcct gaccttactg ctcggcgcat tgtgcgggct
     5341 gatcaacgcc ggattaatta tctataccaa agttaacccg ctggtgatta cgcttggcac
     5401 gctgtatctg tttgccggaa gcgctctgct gctttccggt atggccggag cgacggggta
     5461 cgaaggtatt ggtggattcc cgatggcgtt tacagatttc gctaacctgg atgtgctggg
     5521 actccccgtt ccgctgatta tcttcctgat atgtctcctc gttttctggc tctggctgca
     5581 taaaacccat gccggacgta atgtgttttt gattgggcaa agcccgcgcg tggcgcttta
     5641 tagcgcgatt ccagttaacc gcaccttatg tgcgctctat gccatgacgg ggctggcgtc
     5701 tgcggtcgcc gctgtgctgc tggtatcgta ttttggttca gcacgttccg atctcggtgc
     5761 gtcgtttctg atgcccgcca tcaccgccgt ggtgcttggc ggggccaata tttatggtgg
     5821 ttccggttcc attatcggca ccgccattgc ggttttatta gtgggatatt tgcagcaagg
     5881 tttgcaaatg gcaggagtgc caaatcaggt gtccagcgcc ctttccggtg cgctgcttat
     5941 cgtcgttgtc gtaggtcgtt ccgttagcct gcatcgccag caaattaaag agtggctggc
     6001 gcgtcgggcc aataacccat tgccataaag gatatcttca tgacacttca tcgctttaag
     6061 aaaatcgcct tacttagcgc tcttggcatt gccgcaatct ctatgaatgt gcaggccgca
     6121 gagcgtattg catttattcc caaactggtt ggcgtgggat tttttaccag cggtggcaac
     6181 ggcgcacaac aagcgggtaa agagctgagc gttgatgtga cctacgacgg gccgacagaa
     6241 cccagtgttt ctggtcaggt acagttgatt aataacttcg tcaatcaagg ttataacgcc
     6301 attattcgtt tctgcggttt cgcctgatgg cttgtgtccg gcactgaaac gcgccatgca
     6361 acgtggtgtg agagtgctga cctgggactc tgatactaaa ccggagtgcc gctcttacta
     6421 cattaatcag ggaacgcccg cccagttggg aggtatgttg gtggatatgg cggcgcgtca
     6481 ggtgaataaa gacaaagcca aagtcgcgtt tttctactca agccccaccg ttacggacca
     6541 aaaccagtgg gtgaaagaag cgaaagcgaa aatcgccaaa gagcatccag gctgggaaat
     6601 tgtcactacg cagtttggct ataacgatgc cactaaatcg ttacaaaccg cagaaggaat
     6661 attaaaagcg tatagcgatc tcgacgccat tatcgccccc gatgccaacg ccctgcccgc
     6721 tgccgcacaa gccgcagaaa acctgaaaaa tgacaaagta gcgattgtcg gattcagtac
     6781 gccaaatgtg atgcgcccgt atgtagagcg cggcacggtg aaagaatttg gactgtggga
     6841 tgtggttcag caaggcaaaa tttcagtgta tgtcgcggat gtattattga aaaaaggatc
     6901 aatgaaaacg ggcgacaagc tggatatcca gggcgtaggt caggttgaag tctcgccaaa
     6961 cagcgttcag ggctatgact acgaagcgga tggtaatggc atcgtactgt taccggagcg
     7021 cgtgatattc aacaaagaga atatcggcaa atacgatttc tgatgtgcat tacttaaccg
     7081 gagtaagtta tggcagattt agacgatatt aaagatggta aagattttcg taccgatcaa
     7141 ccgcaacaaa atatcccttt taccctgaaa ggttgcggtg cgc
//
LOCUS       DAZPCS010000175         7168 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZPCS010000175 DAZPCS010000000
VERSION     DAZPCS010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7168
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.177"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            176..1747
                     /gene="garD"
                     /locus_tag="ACHUQ7_003493"
     CDS             176..1747
                     /gene="garD"
                     /locus_tag="ACHUQ7_003493"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFR1136746.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMHCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMAPRTELANRWFDLMDINAGTIATGEETIEEEGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            complement(1799..2623)
                     /gene="agaR"
                     /locus_tag="ACHUQ7_003494"
     CDS             complement(1799..2623)
                     /gene="agaR"
                     /locus_tag="ACHUQ7_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFR1136747.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKYTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLSRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            2872..4152
                     /gene="kbaZ"
                     /locus_tag="ACHUQ7_003495"
     CDS             2872..4152
                     /gene="kbaZ"
                     /locus_tag="ACHUQ7_003495"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFR1136748.1"
                     /translation="MKHLTEMVRQHKAGKTNAIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            4175..4648
                     /gene="agaV"
                     /locus_tag="ACHUQ7_003496"
     CDS             4175..4648
                     /gene="agaV"
                     /locus_tag="ACHUQ7_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFR1136749.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            4659..5438
                     /gene="agaW"
                     /locus_tag="ACHUQ7_003497"
     CDS             4659..5438
                     /gene="agaW"
                     /locus_tag="ACHUQ7_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFR1136750.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGDTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAATW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            5428..6237
                     /gene="agaE"
                     /locus_tag="ACHUQ7_003498"
     CDS             5428..6237
                     /gene="agaE"
                     /locus_tag="ACHUQ7_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFR1136751.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDRSIAAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRG"
     gene            6324..6758
                     /gene="agaF"
                     /locus_tag="ACHUQ7_003499"
     CDS             6324..6758
                     /gene="agaF"
                     /locus_tag="ACHUQ7_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFR1136752.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            6755..>7168
                     /locus_tag="ACHUQ7_003500"
     CDS             6755..>7168
                     /locus_tag="ACHUQ7_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFR1136753.1"
                     /translation="MTHVLRARRLLTEEGWFDDHQLRVADGVIAAIEPIPVGVTERDA
                     ERLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHP"
BASE COUNT         1697 a         1864 c         1907 g         1700 t
ORIGIN      
        1 agcacagatt tgcgctttac cttgccgggc ggcactgcaa tccctaaaat gattgacatt
       61 gatcacattt ctgcgtttaa actcctgaca ttcttatttc acccaatgaa atcatttatt
      121 tttaaatgag accaggtcct cattttaata acccctggct ggagaatatt gcacaatggc
      181 caacatcgaa atcagacaag aaacgccaac tgcgttttat ataaaagttc acgacacaga
      241 taatgtggca attattgtta atgataatgg cctgaaagca ggaacgcgtt ttccggatgg
      301 gctggaatta attgaacata ttccccaggg gcataaagtc gcattgctgg acattccggc
      361 taatggtgaa attattcgtt atggcgaagt gattggttac gccgtgcgtg caatcccacg
      421 cggaagctgg atcgacgaat caatggttgt actaccggaa gcgccgccgt tacacacgct
      481 gccactggca accaaagtcc cggaaccctt accgccgctg gaaggataca cctttgaggg
      541 ctatcgcaat gccgatggca gcgtgggcac caaaaacctg ctcggcatca ccaccagcgt
      601 ccactgtgtg gcaggcgtgg tggactatgt agtaaaaatc attgaacgcg atctgctacc
      661 gaaatacccg aacgtcgatg gcgtggtggg gctgaatcat ttgtacggtt gtggcgtggc
      721 gattaacgca ccggcagcag ttgtacctat tcgtaccatt cacaatattt cgctgaaccc
      781 taactttggc ggcgaagtaa tggtgattgg cctgggttgt gaaaagttgc agcctgagcg
      841 cctgctgact ggaacggatg atgtgcaagc tattccagta gaaagcgcca gcattgtcag
      901 tttgcaggat gaaaagcatg tcggttttca gtccatggtc gaggatattt tgcaggtcgc
      961 cgaacgccat ctacaaaaac tgaatcaacg tcagcgagaa acctgtccgg cttcagaact
     1021 ggttgtcggc atgcattgcg gtggcagcga tgcgttttct ggcgtaacgg caaacccggc
     1081 ggttggctat gcgtctgatc tactggtgcg ctgcggcgca acggtgatgt tttcagaagt
     1141 cacggaagtg cgtgacgcga tccatctgct gacaccacgc gcagtgaacg aagaggtcgg
     1201 caaacggctg ctggaggaga tggagtggta cgataactat ctcaatatgg gaaaaaccga
     1261 ccgcagcgcc aacccttcgc cgggcaacaa gaaaggcggt ctagcaaacg tggtagagaa
     1321 agcgctcggc tccattgcta aatcgggcaa aagtgcaatt gttgaagtgc tgtcgcccgg
     1381 tcaacgcccg actaaacgcg gattaattta cgccgcgacg ccagccagcg attttgtctg
     1441 tggcacgcaa caggtggctt cgggtatcac cgtgcaagtg tttacgaccg gtcgtggtac
     1501 gccgtacggc ctgatggcgg tacccgtcat taaaatggca ccccgcaccg agctggcgaa
     1561 ccgctggttt gatttaatgg atattaatgc gggcaccatc gctaccggcg aagaaactat
     1621 tgaagaggag ggctggaagt tgttccactt tattctcgac gtcgccagcg ggaagaagaa
     1681 aaccttctcg gatcaatggg gactgcataa ccagctggcg gtgtttaacc cagcaccggt
     1741 gacctgataa ttcaattcgt attatgcccg atagcacacg tttatcgggc atattgagtc
     1801 acagcgaaga cgcgtgatcc ccgaccagaa tcacttcaac cccagccttt cgcagtcctt
     1861 ccagactatc cgcaggaatg ccttcatcaa caatgatcat gtcgatacgt tgagtatcaa
     1921 tgatcttatg taaactggaa cgattgaatt tactggaatc ggtgaccacg atgatccgtt
     1981 ccgcaacttc gcacatccga cggcttaaac gggcttcatc ttcattatgt gtgctgacgc
     2041 cgcgctccag atcgatcgca tctacaccaa gaaacagcat atcgaagtgg taattttgca
     2101 gcgattgctc agcctgatcg ccgtaaaaag attgcgactg acggcgcaaa tgcccgccgg
     2161 tcatcagcag ctcaacgcct tccgcttcca gcaacgcatt agccacgttc ataccgttgg
     2221 tcatcgcaat tacgtcagtg tacttgcgca tcagacgagc aatctcaaaa gtggtggtcc
     2281 cggaatcgag gatcacccga tgacctggct gaatcaactc aacggcagct ttcgcaacgc
     2341 tgcgtttcat cgcggtgttc agtgcgcttt tatcttccac tgatggctcg actgacggcg
     2401 tcgtgctatc gcagatcaac gcgccaccat aggcacgcac agcgatcccc tgcttttcca
     2461 gaaacgccag atcattgcgg atcgtcacag tagatacgcc atacaatgcc gacagatcgt
     2521 taacctgcac actcccttgc tgtcgcagac gctgaatgat ctgttctcgt cgctcgctgg
     2581 tgcctgtcac tcgcttctca cctgaagcgt cggtattact catagtaagt cctttcgtaa
     2641 aactttcgtt tcatttcgtt ttgcctatta acgcctttct attaagcaaa tgcaagccca
     2701 ccttgcccat tggcgcaagc tactctcgtt tcagtgactt tcattacgtt tcttttgtga
     2761 atcagatcag aaaactatta tctttcgttt tatttttatc tcaccatgac gcagtatcaa
     2821 ttgaaacaaa acgaaagatc aatatcgcag taatctgaac tggagaggaa agtgaaacat
     2881 ctgacagaaa tggtgagaca gcacaaagcg ggcaaaacaa atgcaattta tgccgtttgt
     2941 tccgcacatc cgctggtgct ggaggctgca atccgctacg ccagtgcaaa ccaaacgccg
     3001 ttactgattg aagcaacctc caatcaggta gaccagttcg gcggttatac cggaatgacg
     3061 cccgccgatt ttcgcggctt tgtttgtcag ctcgccgact cgttgaattt cccgcaggat
     3121 gcgctgattc tgggtggtga ccatctgggg ccaaaccgct ggcaaaacct gccagccgct
     3181 caggcaatgg ccaatgccga tgatttgatt aaaagctacg ttgcggcagg attcaaaaaa
     3241 atccaccttg attgcagcat gtcctgtcag gacgatccaa ttcccttaac tgatgacatc
     3301 gtggctgaac gcgccgcccg tctggcgaaa gtggcggaag aaacctgtcg cgaacacttt
     3361 ggcgaagccg atctggagta tgtcattggt accgaagtgc cggtacctgg cggcgcgcat
     3421 gaaaccttaa gcgagctggc ggtcaccacg ccggatgccg cccgcgccac gctggaagcc
     3481 catcgtcacg cctttgaaaa gcaaggtttg aatgccatct ggccacgcat tattgccctg
     3541 gtggttcaac ccggcgtcga attcgatcac accaacgtta ttgattatca gcccgccaaa
     3601 gcgagcgcct taagccagat ggtcgaaaac tacgaaacgc tgattttcga agcgcactct
     3661 accgattatc aaacgccgca atctctgcgc cagctggtga ttgaccactt tgccattctg
     3721 aaagttggcc cggcgctgac cttcgccctg cgtgaagccc ttttttctct ggcggcgatt
     3781 gaagaagaac tggtaccagc gaaagcctgt tccggtctgc gtcaggtgct ggaaaacgtg
     3841 atgctcgacc gcccggaata ctggcaaagc cactaccacg gtgacggcaa cgcgcgtcgt
     3901 ctggcgcgtg gttacagcta ctcagatcgc gtgcgctatt actggccgga cagccagatt
     3961 gatgacgctt tcgctcatct ggttcgtaat ctggcggatt caccaattcc gctaccgctc
     4021 atcagccagt atctgccgct gcagtacgtg aaagttcgct ccggcgagct gcaaccgacg
     4081 ccacgggaac tcattataaa ccatattcag gacatcctgg cgcagtacca cacagcctgt
     4141 gaaggccaat aagcaaaaca aagaggaaca tgctatgcca aatattgttt taagccggat
     4201 tgatgaacgc ttgattcacg gtcaggtcgg cgttcagtgg gtcggatttg cgggggcaaa
     4261 tctggtgctg gtagccaacg atgaggttgc cgaagatccg gtacaacaaa acctgatgga
     4321 aatggtactg gcagaaggga tcgccgtacg tttctggacg ctgcaaaaag ttatcgacaa
     4381 cattcatcgc gccgccgatc gacagaaaat cctgctggtt tgtaaaacac ccgccgattt
     4441 cctgacgctg gtgaaaggtg gcgttccggt gaatcgcatt aacgttggca atatgcacta
     4501 cgccaatggc aaacaacaaa tcgccaaaac ggtttctgtg gatgcgggcg atatcgcagc
     4561 atttaacgac ctgaaagccg ctggggtgga atgcttcgtt cagggcgtcc cgacagagcc
     4621 tgctgtggac ctctttaaat tactttgagg gattcatcat ggaaatcagc ctgttgcagg
     4681 catttgcgtt gggcattatc gcctttatcg ctggcctaga tatgtttaac ggcttaaccc
     4741 atatgcaccg cccggtggtc ctcggcccgt tggtcgggct ggtacttggc gatctgcata
     4801 ccggaatttt aaccggcgat acgctggaac tagtgtggat ggggctggcc ccgctggcgg
     4861 gcgcacagcc gcctaacgtg attatcggta ctatcgtcgg cacggcgttt gccattacta
     4921 ctggcgtgaa acccgatgtc gcggtcggtg tcgccgtacc tttcgctgtc gcagtacaga
     4981 tggggattac cttcctgttc tcggtgatgt ctggcgtgat gtctcgctgc gaccggatgg
     5041 cggagaacgc cgatactcgc ggcattgaac gcgtgaacta tctggcactg ctggcactcg
     5101 gcactttcta ttttctctgc gctttcctgc ctatctactt cggcgcggaa catgcaaaaa
     5161 ccatcattga tgtcctgccg caacgattaa tcgacggcct cggcgtcgcg ggcggcatca
     5221 tgccagcaat cggttttgcc gtgctgctga aaatcatgat gaaaaacgtc tacataccct
     5281 acttcatcct gggctttgtt gccgcaacct ggctcaagtt accggtgctg gctattgctg
     5341 ccgccgcgct ggcgatggcg ctgatcgatc tgctgcgtaa atctcctgaa ccgactcaac
     5401 ctgcggcaca gaaagaggaa tttgaagatg gcatctaatc aaaccaccct gccgaacgtc
     5461 agcgaaaatg aagaaacact gctgactggc gtcaatgaaa acgtgtatga agatcggagc
     5521 attgccgcgg agctgacgaa aaaagatatc aaccgcgtcg cctggcgttc catgctgtta
     5581 caagcgtctt ttaactacga acgtatgcag gcttctggct ggttgtacgg tctgctgcct
     5641 gctctgaaaa agatccacac taataaacgc gacctggcgc gcgccatgaa ggggcatatg
     5701 ggtttcttca atacccatcc gtttctggtg acgtttgtta tcggcattat ccttgcgatg
     5761 gagcgttcta agcaggacgt taacagtatt cagagcacca aaattgccgt cggtgcgccg
     5821 cttggcggga ttggcgatgc gatgttctgg ctaacgctac tgccgatttg tggcgggata
     5881 ggcgcaagcc tcgcgctaca aggctctatt cttggcgcag tcgtctttat tgtgctgttc
     5941 aacgtggtac acttggggct gcgttttggt ctggcgcatt atgcttaccg catgggcgtg
     6001 gcggcgattc cccttattaa agcgaatacc aaaaaagtcg gccatgctgc gtcaatcgtt
     6061 ggaatgacgg taatcggcgc actggtggca acctatgttc gtttaagcac cacgctggaa
     6121 atcacggcgg gcgacgcagt agttaagtta caggctgatg ttatcgacaa actgatgcct
     6181 gccttcttac cgctggtcta caccctgacc atgttctggc tggtacgccg cggctagagt
     6241 ccgctgcgcc tgattgcggt taccgtggtt ctagggatcg tcggtaaatt ctgccatttc
     6301 ctttaaatac aatgaggtat tagatgttaa gtattatttt gacagggcat ggcgggtttg
     6361 ccagtggcat ggaaaaagca atgaagcaaa tcctcggcga tcaatcgcag tttattgcca
     6421 tcgattttcc cgaaacctca agcgccgcgc tgcttacatc gcagcttgaa gaggccatcg
     6481 cccaactgga ttgtgaagac ggcattgttt ttctgacgga tttgctgggc ggcacgccgt
     6541 ttcgcgtagc ttcaacgctg gcgatgcaaa aaccgggctg tgaagtgatt accggcacca
     6601 atttgcaact gctactggag atggtgctgg agcgcgaagg gttaagcggt gaagaattcc
     6661 gtgtacaggc gctggagtgt ggacaccgag ggctgaccag tctggtggac gagttaggcc
     6721 gctgccatga agaatgtccg gtcgaggaag gaatatgaca cacgttctgc gcgccagaag
     6781 gctgctgact gaagagggat ggttcgatga ccatcagttg cgtgttgctg acggtgtcat
     6841 cgcagcaatc gaaccgattc cagtgggcgt gactgaacgc gatgcggaac ggctctgccc
     6901 cgcttacatc gacacccatg tacacggtgg tgcgggcgtt gatgtaatgg atgacgcgcc
     6961 ggatgtactc gacaagctgg caatgcacaa ggcacgcgaa ggtgtcggca gttggttacc
     7021 gaccaccgta accgcgccgc ttaataccat tcatgcggcg ctgaaacgta ttgctcaacg
     7081 ttgccaacgc ggcggacctg gtgcgcaagt gctggggagt tatctcgaag gaccgtactt
     7141 cacgccgcag aataaaggcg cgcatccg
//
LOCUS       DAZPCS010000176         7096 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZPCS010000176 DAZPCS010000000
VERSION     DAZPCS010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7096
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.178"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..108)
                     /locus_tag="ACHUQ7_003501"
     CDS             complement(<1..108)
                     /locus_tag="ACHUQ7_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1136754.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIY"
     gene            186..1280
                     /gene="ddlA"
                     /locus_tag="ACHUQ7_003502"
     CDS             186..1280
                     /gene="ddlA"
                     /locus_tag="ACHUQ7_003502"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFR1136755.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1304..1516)
                     /locus_tag="ACHUQ7_003503"
     CDS             complement(1304..1516)
                     /locus_tag="ACHUQ7_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFR1136756.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1776..2084
                     /locus_tag="ACHUQ7_003504"
     CDS             1776..2084
                     /locus_tag="ACHUQ7_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFR1136757.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2143..3237)
                     /gene="yaiW"
                     /locus_tag="ACHUQ7_003505"
                     /pseudo
     CDS             complement(2143..3237)
                     /gene="yaiW"
                     /locus_tag="ACHUQ7_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
     gene            complement(3250..4470)
                     /gene="sbmA"
                     /locus_tag="ACHUQ7_003506"
     CDS             complement(3250..4470)
                     /gene="sbmA"
                     /locus_tag="ACHUQ7_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HFR1136758.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4822..5979
                     /gene="ampH"
                     /locus_tag="ACHUQ7_003507"
     CDS             4822..5979
                     /gene="ampH"
                     /locus_tag="ACHUQ7_003507"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFR1136759.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITHPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPRIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(5980..6483)
                     /locus_tag="ACHUQ7_003508"
     CDS             complement(5980..6483)
                     /locus_tag="ACHUQ7_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1136760.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
     gene            complement(6571..>7096)
                     /locus_tag="ACHUQ7_003509"
     CDS             complement(6571..>7096)
                     /locus_tag="ACHUQ7_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR1136761.1"
                     /translation="LSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKTGYDFKLGDA
                     GYVTPYGSISGLFQSGDDYQLSNDMKVDGQPYDSMRYELGVDAGYTFTYSEDQALTPY
                     FKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDV
                     DQDWSANVGVKYTW"
BASE COUNT         1793 a         1815 c         1719 g         1769 t
ORIGIN      
        1 ataaattagc ttcccgttca tcatttttgt gatcttgcgc acatctttga gataacgcac
       61 tttcttcacc agtacgttgc acgctttgtc agcagaagat tgcaacaatc tttcagaagg
      121 ccgcgaatcc cccgttttgt cgtggtaatg tatgcacttt gtgttagacg ggataggttt
      181 ttaagatgga aaaactgcgg gtaggaatcg tttttggtgg taaatcagcg gaacatgaag
      241 tgtctctgca atcggcaaaa aacattgtcg atgccattga taaaagtcgc ttcgacgttg
      301 tgctgctggg cattgataaa caagggcaat ggcacgtcag cgatgccagc aattatctgc
      361 taaatgcaga cgatcctgcc catattgcgt tgcgcccttc ggcgaccagc cttgcgcagg
      421 tgccaggtaa acatgagcat cagcttatcg acgcgcaaaa cggtcagccg ttgccgacgg
      481 tggatgtcat tttcccgatt gtccacggta cgctgggcga agatggttcc ttgcagggaa
      541 tgctgcgggt cgccaattta ccgtttgtag gttctgatgt tctggcttca gcagcctgta
      601 tggataaaga tgtcaccaaa cgtctgctac gcgatgccgg gctgaacatt gcgccattta
      661 ttaccctgac gcgcgctaat cgtcacaaca tcagttttgc cgaagtggag tctaaactgg
      721 ggttaccact gtttgtaaaa ccggctaatc agggctcttc tgttggtgtc agcaaagtaa
      781 ccagtgaaga acagtacgca attgccgtcg atctggcgtt cgagttcgat cataaagtga
      841 tcgttgagca agggatcaaa ggtcgtgaga tcgaatgcgc agttctgggc aacgacaatc
      901 cgcaagccag cacctgtggc gagatcgtac tcaccagcga tttctatgcc tacgacacca
      961 agtacattga cgaagatggc gcgaaagtgg tagttccggc agccattgca ccagagatca
     1021 acgataagat ccgggcgatt gccgttcagg cttatcagac gttgggatgc gcaggcatgg
     1081 cgcgtgtaga cgtgttttta accccagaga acgaagtggt gatcaacgag atcaacacac
     1141 tgcctggctt caccaacatc agtatgtatc cgaagctgtg gcaagccagc ggtctgggtt
     1201 acaccgatct gatcacacgt ttgattgagc tggcgctgga gcgtcacgct gcggataacg
     1261 cattgaaaac cacaatgtaa tattttaacg cccgataata ttcttattgg gcgttttcag
     1321 tttcttcatc gcgacgacga ataatcaacc ccgccagcca gaaactaatc acccacgtca
     1381 ccagaccgac ggcataggtc tgccaacctt ttgcttcaaa tcccagtacc cccaccacgc
     1441 cattaagaat gaatataagg ccaatggcga acgcatagta gtgccagtca cggcggattt
     1501 ttacaggcag attcatggca gctccgttaa cgaaaaacaa catccttgca tattctgtaa
     1561 caaccgtctg tggtgtagac aacattttcg taattattaa taaatttaac ttaaggttgc
     1621 cattttgtga ctcacaacga agattcacgc ttcgggaata ttcccaacat caatttacca
     1681 tgattctgat attgacaatc taaatgacct gtcagacttg ccataagccg ctggtatcac
     1741 accagaagag aagcagatga cttccggagg tttttatggc tgatttcacc ctgtcaaaat
     1801 cgctgtttag cggaaaatat cgcaatgcct cttcaacgcc tggcaacatt gcctatgcgt
     1861 tgtttgtgct gttttgcttt tgggctgggg cgcaattgct gaacctgtta gtgcatgcgc
     1921 ccggcgtcta tgagcgttta atgcaggtcc aggaaacagg tcgcccacgg gtggaaattg
     1981 gtttaggtgt cggcaccatt ttcgggctga tcccgttttt agcaggctgc ctcatttttg
     2041 cagtggtggc gctatggctg cactggcgac atcgccgcca gtaaaattta cgttgccaga
     2101 tgcggcctga aacgccttat ctggcctgac attcctgttt gatcactgtt tcatacaacg
     2161 cgcccgccgt tcgtctacgc gcttcgcaaa ccaggccgta gtcaggttgc gggtgatttt
     2221 cgggctttcc agttgaatgc caggcaacat ttcgcggggt agtgatttac ccgttttcgc
     2281 ttcggcaagt tgataaactt tcttgtacag cgccgtctcc tcaaaagaga agctgtcacc
     2341 tttctctaac tgacggcgaa tttcgctgtc gctcatcccc agttttcccg ccagtttgcg
     2401 cgttgccagt tcggttttcc cgggttcttt actgtcgtaa cgaattaaat caccgtccag
     2461 tgccagcttc acgccgctgg ccttactgac tgcgttctga aacgcggcat tacggctggc
     2521 gtaccagcca gcgttaaaat cagcaaagcg gtatatcggt gcgctatagc tggcgggata
     2581 attcagtaaa tggtaagtac caaaccacaa cccgccgcgg cgactgaaga cttcctgacg
     2641 aactgtaccg tccattttcc acggataccc tttggtatgc tgttcggcaa aagcaatgct
     2701 gacctgcatc ggcccaccgg tgcgcaccgg attaagcgaa ccaaacagcg tctgccccat
     2761 tggtaccatg ctgatcagat cgtcaaaaat cgcgctcaat tgcttttctg tacgcaccga
     2821 atctaaccgt tcgctataac ttttgccgtt tggcgatttg atttttagcg cggtatgaac
     2881 cagaaaagca ggaatgtgca tccgttcagc acgacggtca atctcttgct aggcgatctt
     2941 gcttaagccc ggaacggccg gatctgcctg ataatttgac tcctgctgcg ccaccgccag
     3001 caccgagtag acgttttcca gcgtcggtgc cagcccctga ctttcaaagg tggtggcgag
     3061 atctttcgcc caggcatcgc ggtctttcac gctcgcaggc atcttctgcc gcacgacgct
     3121 cgctacatct atcgcttttt cgcccttttt caacggttgc ggagcctgac tactacaccc
     3181 cgctaaaacc agaaccgcca acaatgacag cgaagataga tgattgacgc gcgacattac
     3241 ttctcctttt tagctcaagg tatgggttac ttcctgaatt ttgtcaccat ccagctcatg
     3301 ttcaaagctg cgcagacgtt tgtagataga catcaactca accagtgtgg tccatgagtt
     3361 aatcaggtac tggaaagcac cgcgaacctg accaaaaacg ttggtaatct gcgtcatcag
     3421 gccgagcgta atcgtaccgg caacaattga cggaaacagc aagaacaaac cgaaaacgtt
     3481 atcgacctgc aaatagagaa tgcgggcgat gttgaaatac atatagtgaa aatagaggcg
     3541 aaaatagttt ttccgtacgg cgctaaacag ctcgcgtacc gtaggcggcg tcgcgcgcgt
     3601 ggcatcgtct tcaccataaa ccagctcttt acggtaggcg gcctctactc gctggttttt
     3661 aaactccagc cccggcagtt tgatccctac cactgccagc aatccggtcc ccatcagcga
     3721 ccagacgata gcggcaatca ccagaccata cggaatgtgc ccgataatcg gcagctccgg
     3781 cacatgcgcg gagagcgtta ccagcaccgg caggaaggcg atcaacgtca tgatggcgtt
     3841 gataaaactg acgcccatat tctccagcgt tgaagcaaaa cgcatggtgt cttcctgcac
     3901 acgctgtgcg gcaccttcga tatgacgcag ttgttgccag ttcgccatgt aatattcgtt
     3961 catcgccgta cgccagcgga acacgtagtg actgacaaag aagttgttca gtacactgat
     4021 caccaccgcg atcagcgcaa tccccagaaa gacgcccact tcgcggtaaa attgttcgat
     4081 agtgacttta tgcggcgaac ttagcgcggt ttgaatcaga tcatagaacg gcgcatacca
     4141 ggcgttgacg gcgaccccga cttccaccaa aaaccaagtg acgaagataa tcagcgcagt
     4201 accgagtatt gaccagtatt gccaacggtg cgggctgtag ataaaccaga aaaatgcaaa
     4261 aagtcctacg caaacaatgt agtaagcgta aaaaatcagg aaatccaacg accagaaacg
     4321 cgcggcgcta atcgggatct gcccggaagc gccggtgata cgtgccaccc agtcaccccc
     4381 accggcttgc cagaagataa cggcaatcaa tgcccaaaca aaggccgaga gaaaaaacgt
     4441 tcccggcttt gggaaaaaag acttaaacat atgcactcct gctaacttct tatcgtttta
     4501 acaactgctg tgtattaacc gcatgaccgc tacaaagcgt cagccaaatg aaaccgccca
     4561 ttgtgaccca tagcaaggcg cttagttcgc cagcctgacc ataagaattg tttcgacgga
     4621 tttcacaaac caaagattgt ttacataagc ggattaacac gcgcaggatc acaaatgcag
     4681 gcattggtga gcaggaaaaa gatcgtgttt acgcgccgtt acgtttttaa cactattatt
     4741 gcactaattc gccctttgca atccatcaat gagtagtata aatacgctca gttaccttca
     4801 ttcaatctat ggacaccacc gttgaaacgt agtctgcttt tttctgccgt gctgtgtgcg
     4861 gcgtcattga cctccgtcca tgcggcgcag ccgatcactg aaccggagtt tgcctctgat
     4921 attgtcgatc gttatgccga tcatattttt tacggcagcg gtgccacggg gatggcgctg
     4981 gttgttatcg acggtaacca gcgcgtcttt cgtagttatg gtgaaacgcg acctggtaat
     5041 aacgttcgcc cgcagctgga ttcggtcgtg cgtattgctt ccctcaccaa actaatgacc
     5101 agtgaaatgc tggtgaaatt gctcgaccag gggaccgtaa agttaaacga tccgctaagt
     5161 aaatatgctc cgccaggcgc acgcgtgccg acctacaatg ggacgcccat taccctagtg
     5221 aatctggcaa cccataccag cgccctgccc cgtgaacagc ccggtggcgc ggcacatcgt
     5281 ccggtgtttg tctggccaac gcgcgagcaa cgctggaaat acctttctac ggcgaagctg
     5341 aaagccgcgc caggcagtca ggcggcgtac tctaaccttg cgttcgacct gctggcagat
     5401 gcactggcga atgcgtccgg caagccttat acccagttgt ttgaagagca gattacccat
     5461 ccattgggga tgaaagacac cacctacacc ccctcaccgg atcagtgccg ccgtttaatg
     5521 gttgctgagc gcggtgccag tccgtgcaat aacacgctgg cggcaattgg cagcggcggc
     5581 gtctattcca cgcctggcga tatgatgcgc tggatgcagc agtatttgtc atctgatttt
     5641 tatcagcgca gtaaccaggc agatcgcatg cagacgttga tttatcagcg cgcgcagttt
     5701 acgaaagtga ttggcatgga tgtccccggc aaagcggatg cgcttggttt aggctgggta
     5761 tatatggccc cgaaagaggg tcgtccgagg attattcaga agacaggcgg tggtggcggt
     5821 ttcattacct atatggcgat gatcccgcag aagaatattg gcgcgtttgt ggtggtcact
     5881 cgttcgccgt tgacgcgctt taaaaatatg agcgatggca tcaatgatct ggtgaccgag
     5941 ctaagcggga ataaaccgtt ggttatcccc gcatcctgat taatactctg aaggcaaacg
     6001 gttgatagcg accagtttgc ctttattcat ttcgatatag ccgcctttac gcaaagcagc
     6061 gagaacttct gcgacgacag aacgcgatat gcgtgtacgt tgatggatat aattcatcac
     6121 accaatacgt gatcgcaatt cttcattcca gtcaatcatt gataataatg ttgcgcgggt
     6181 ttgtttggct ggtagatgat atcccgtaca atttccttct gatattaatt tgtatggtat
     6241 atcgtttttc attaaaccat cagccagccc cataatataa ggagcctggg taataccgat
     6301 aagtacgttt tcttctctac gcagagagat aactccttct aaaataacga aagtatcttc
     6361 gttattgaca tcactggaaa atataacttg cttttcatta ttaaactcga agcgcgtacc
     6421 gtatctggac aaacatttat cgagcttacc aaattcctga agaggtttaa ctacagataa
     6481 catttgcgcg tcctttgcag taatgcccgt caaatccttg acgggcatta tttagattaa
     6541 attaccagta tttcttcgga gtgaagaata ttaccaagta tatttaacac ccacgttcgc
     6601 ggaccagtct tgatctacgt caccaccacc gaggtagtta gcatcggtat aggcgctgaa
     6661 gttcttggtg aagctgaact gagtacccag accaacacgt accgcagacc cttcagtacc
     6721 gttatcgatg gaatcaccgt tcacatcgtt atcgttgtta gagtcgtcgt agacgtaagc
     6781 cagtttgaag tacggagtca gagcctgatc ttcgctgtag gtgaaggtat aacctgcatc
     6841 tacacccagt tcataacgca tgctgtcgta aggctgaccg tcaactttca tgtcgttgct
     6901 cagctggtag tcatcaccag actggaacag accagaaatg ctgccgtaag gagtcacgta
     6961 accagcatca cccagtttga agtcgtaacc ggttttcaaa ccgaagcccc aagcgtcgga
     7021 gttggtgcta ccgtcaacgt aagtaccgtt gctcatggtt gcagacaggt cgttgttgaa
     7081 gtgagagtag ctcaag
//
LOCUS       DAZPCS010000177         7069 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZPCS010000177 DAZPCS010000000
VERSION     DAZPCS010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7069
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.179"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            1..4865
                     /gene="traI"
                     /locus_tag="ACHUQ7_003510"
                     /pseudo
     CDS             1..4865
                     /gene="traI"
                     /locus_tag="ACHUQ7_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
     gene            4885..5631
                     /gene="traX"
                     /locus_tag="ACHUQ7_003511"
     CDS             4885..5631
                     /gene="traX"
                     /locus_tag="ACHUQ7_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HFR1136762.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            5686..6246
                     /locus_tag="ACHUQ7_003512"
     CDS             5686..6246
                     /locus_tag="ACHUQ7_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFR1136763.1"
                     /translation="MTEQKRPVLTLKRKTEGTAPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            6377..6589
                     /locus_tag="ACHUQ7_003513"
     CDS             6377..6589
                     /locus_tag="ACHUQ7_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFR1136764.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            6833..>7069
                     /locus_tag="ACHUQ7_003514"
     CDS             6833..>7069
                     /locus_tag="ACHUQ7_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFR1136765.1"
                     /translation="MRKYIPLALFIFSWPVLSADIHGRVVRVLDGDTIEVMDSLKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVT"
BASE COUNT         1631 a         1773 c         2316 g         1349 t
ORIGIN      
        1 acggtgctga ccggtaatct ggtgatggca ctgtttaacc acgacaccag tcgcgatcag
       61 gaaccacagt tacacacgca tgcggtggtg actaatgtta cgcagtataa cggcgaatgg
      121 aagacactga gcagtgacaa agtggggaaa acggggttca ttgagaatgt gtacgctaat
      181 cagattgcct ttggcaggct ctaccgggaa aaactgaaag agcaggttga ggcgctgggc
      241 tatgaaactg aagtggtcgg taagcacggt atgtgggaaa tgccgggtgt accggtggag
      301 gccttttccg gacgctcaca gactatccgg gaggccgtcg gggaagacgc ctcgctgaaa
      361 tcccgggatg tggcggcgct ggatacgcgt aaatccaaac agcacgtcga tcctgaagtc
      421 agaatggccg agtggatgca gacgctgaag gaaaccgggt tcgacatccg ggcatatcgt
      481 gacgcagcgg aacaacgggc atatacccgc acgcagacac ccggacccgc ttcacaggac
      541 gggccggatg tgcagcaggc ggtgacacag gccattgccg gattaagtga acgcaaagtg
      601 cagtttatgt atacggacct gctggccagg acggtcggca tactgccgcc ggaaaatggt
      661 gtgattgaac gggcacgcgc cggtatcgat gaggccatca gccgtgagca gcttatcccc
      721 ctcgatcgtg agaaggggct gttcacgttc gggattcata tgctcgatga gctgtcagtc
      781 cgggcactca gtcgtgacat catgaaacag aaccgggtga ccgtacatcc ggagaaaagt
      841 gtcccccgga cggccggtta cagcgatgcc gtcagtgttc tggcacagga ccgtccgtcg
      901 ctggccattg tgtccgggca gggcggtgca gccgggcagc gtgagcgggt ggctgaactg
      961 gtcatgatgg cccgggagca ggggcgggag gtgcagatta tcgctgctga ccgtcgctcg
     1021 cagatgaacc tgaagcagga tgaacggctg tccggtgaac tgataaccgg acgtcgtcag
     1081 ttgctggaag gaatggcctt cacgccgggc agtaccgtta tcgtcgacca gggcgaaaaa
     1141 ctctccctga aagagacgtt aaccctgctg gatggtgccg cacgtcataa cgtacaggtc
     1201 ctgataaccg acagcgggca gcgaaccggt acaggcagtg cactgatggc catgaaggat
     1261 gccggggtga acacatatcg ctggcagggg ggagaacagc gaccggccac catcatcagt
     1321 gaaccggacc gtaatgtcgg ctggccggag attttgcggc cagcgtgaaa gccggagaag
     1381 agagcgtggc acaggtcagc ggagtacggg aacaggccat actgacacag gccattcgca
     1441 gtgagctgaa aacacagggc gtgctcggac acccggaggt aaccatgacc gccctgtcac
     1501 cggtctggct ggacagccgg agccgttatc tgcgggatat gtaccgcccg ggaatggtga
     1561 tggagcagtg gaacccggag acacgcagtc atgaccgcta tgtgacagaa cgtgtgacgg
     1621 cgcagagtca cagcctgacc ctgcggaatg cgcagggcga aacgcaggtg gtgcgtattt
     1681 cttccctgga cagcagctgg tcgctgttcc ggccggaaaa aatgccggtg gcagacggtg
     1741 agcgtctgag ggtgacaggg aaaattcccg gactccgcgt ctccggcggt gaccgcctgc
     1801 aggtggcatc cgtcagtgaa gatgcgatga cggttgttgt gccgggacgg gctgaaccgg
     1861 ccaccctgcc tgtgagcgat tcaccgttca ctgcactgaa gctggagaac ggctgggtgg
     1921 aaacgcccgg gcattccgtc agcgacagtg cgacggtttt tgcctccgtc acacagatgg
     1981 caatggacaa tgccaccctg aatggtctgg cccgcagtgg tcgtgatgtc cggctgtatt
     2041 cctcactgga tgaaacccgt actgcggaaa aacttgcccg ccatccctcc tttacggtgg
     2101 tttctgagca gataaaggcg cgtgccggtg agacattgct ggaaaccgct atcagtctgc
     2161 agaaagccgg gcttcacacg ccggcacagc aggccattca tctggccctt cctgtgctgg
     2221 aaagtaaaaa cctggccttc agcatggtgg acctgctgac agaggcgaag tcgtttgctg
     2281 cagaaggaac cggttttgct gacctgggag gggaaatcaa tgcgcagata aaacggggtg
     2341 atttactgta tgtggatgtg gcaaaaggct atggcacagg cctgctggtt tcccgtgcgt
     2401 cgtatgaggc agaaaagagc attcttcgcc atattctcga aggtaaggag gcggtcacgc
     2461 cgctgatgga gagagtacct ggcgaactca tggagaaact gacatcagga cagcgcgccg
     2521 ccacccgtat gatactggaa acgtctgacc gtttcacggt ggtgcagggc tatgccggtg
     2581 tgggtaagac cacacagttc cgggcggtga tgtcagccgt gaacatgctg ccggagagtg
     2641 aacgtccccg tgtcgtgggg ctggtgccca cacaccgtgc ggtcggggag atgcgcagcg
     2701 ccggcgtgga tgcacagaca ctggcgtcct ttctgcatga cacgcagctg cagcagcgca
     2761 gcggagaaac tccggatttc agcaacacgc tgttccttct cgatgagagc tctatggtgg
     2821 gcaataccga catggcacgg gcatacgccc tgattgcggc cggtggcggt cgggctgtgg
     2881 ccagcggtga cacggaccag ctgcaggcca tcgcgcccgg tcagcctttc cgtctccagc
     2941 agacgcgcag tgctgccgat gtggtcatca tgaaggagat tgtgcgtcag acgccggaac
     3001 tgcgggaggc ggtatacagc ctgattaacc gggatgtgga aagggcgctg tccgggcttg
     3061 agcgtgtgaa accgtctcag gtgccacgtc tggagggcgc atgggcaccg gagcactccg
     3121 tgacggagtt cagtcacagc caggaagcga aactggcaga agcgcagcag aaggcgatgc
     3181 tgaaaggcga ggcttttccg gatgtcccca tgacactgta tgaagccatt gtccgcgact
     3241 ataccggcag gacaccggaa gcacgggagc agacgctgat tgtcacgcac ctgaatgagg
     3301 accggcgcgt actgaacagc atgattcatg atgcacggga aaaggccgga gaactgggga
     3361 aggtgcaggt catggtgcct gtcctgaaca cagcgaatat acgcgacggg gagctgcgtc
     3421 gtctctccac ctgggagaat aacccggatg cccttgccct ggtggataat gtgtatcacc
     3481 ggattgccgg tatcagtaag gatgacgggc tgataaccct gcaggatgcg gaaggtaaca
     3541 cgcggctgat ttcgccccgg gaggcggtgg ctgaaggcgt cacgctgtac accccggaca
     3601 ccatccgggt ggggaccggt gacaggatac gcttcacgaa gagtgaccgg gagcgcggtt
     3661 atgtggccaa cagcgtctgg acggtgacag cggtttccgg tgacagtgtc acactttctg
     3721 acggtcagca gacccgtgtg attcgccccg gtcaggagcg ggcagagcaa catattgacc
     3781 tggcctatgc catcaccgcc cacggtgcac agggggcgag tgaaaccttt gccatcgcgc
     3841 ttgaaggcac ggaaggtaac cggaaactga tggccggctt tgagtcagcc tacgtggccc
     3901 tgtcgcgtat gaagcagcat gtgcaggtgt acaccgataa ccgtcagggc tggacggatg
     3961 ccatcaacaa tgccgtacag aaaggaactg cccacgatgt gtttgagccg aaaccggacc
     4021 gggaggtcat gaatgcagag cggctgttca gtacggcgcg ggagctgcgg gacgtggcgg
     4081 caggccgtgc cgttctccgt caggcggggc tggccggggg agacagtcct gcacggttta
     4141 ttgctccggg acgtaaatat ccgcaaccgt atgtggcact gccggcgttt gaccgtaacg
     4201 gcaagtccgc cggtatctgg ctgaacccgc tgaccacgga tgacggaaac gggctgcggg
     4261 gattcagcgg tgaaggtcgg gtaaaaggca gcggggatgc gcagttcgtg gccctgcagg
     4321 gcagccgtaa cggagaaagc ctgctggctg ataatatgca ggatggcgtc cggattgccc
     4381 gtgataatcc tgacagtggt gtggtggtaa gaatcgccgg tgaaggccgt ccgtggaatc
     4441 ccggtgccat caccggtggc cgcgtgtggg gggatatccc ggacaacagc gtccagccgg
     4501 gagccggaaa tggtgaaccg gtcacggcag aggtactggc acagcggcag gctgaagagg
     4561 ccatccgccg tgaaacggaa cgtcgcgcag atgaaattgt ccgtaaaatg gcagagaaca
     4621 aacctgacct gccggacggc aaaacagagc aggctgtcag ggaaattgcc gggcaggagc
     4681 gtgaccgggc tgccataact gaacgggaag ccgcgctgcc ggagagtgta ctgcgtgaac
     4741 cacaacgggt gcgggaagcg gtccgggaag ttgcccggga aaatttgctg caggagcgtc
     4801 tgcagcagat ggagcgggat atggttcgtg acctgcagaa agagaaaacc ccgggcggag
     4861 actgatacag gaagacaaac actgatgaca acggataaca cgaacacgac acgtaacgat
     4921 tcactggctg cacggaccga tacctggttg cagtcatttc tggtctggtc acccggacag
     4981 cgggatatca tcaaaacggt ggcactggtg ctgatggtac tggaccacat caatctgata
     5041 ttccagctga agcaggaatg gatgtttctg gccgggcgag gagcctttcc gctttttgcc
     5101 ctggtgtggg ggctgaatct gtcccgtcat gcgcatatcc ggcaaccggc cattaaccgg
     5161 ctgtggggat ggggaattat tgcccagttc gcatattatc tggcaggctt tccttggtat
     5221 gaggggaata tcctgtttgc ctttgcagtg gcggcacagg tgctgacgtg gtgtgaaacg
     5281 cgcagtgggt ggcgtacagc agcggccata ctcctgatgg ccctgtgggg gcctttgtcc
     5341 ggcaccagtt acggtattgc cgggttgctg atgctggcag tcagcaaccg gctgtaccgg
     5401 gcggaagaca gagcggaacg tctggcgctg gtcgcctgcc tgctggctgt tattccggcg
     5461 cttaaccttg ccaccagtga tgcagcggcg gtggccggtc ttgtgatgac ggtgctgacc
     5521 gttggtctgg tgttgtgtgc cggaaaatca ttaccccgtt tctggcccgg agactttttc
     5581 ccgacgttct atgcctgtca tctggctgtg ctgggcgttc tggcgctgtg acgggtgtgg
     5641 tatctttggc cgcaagagga tgattcgtca gaggcagaac acagcatgac agagcagaag
     5701 cgaccggtac tgacactgaa gcggaagaca gagggaacag cgcctgtccg cagccggaaa
     5761 accatcatca atgtcaccac gccaccaaag tggaaggtga aaaagcagaa gctggccgag
     5821 aaagcggccc gtgaagcaga gctggcggca aaaaaagcgc aggccagaca ggcgctgtcc
     5881 atttatctga acctgcccac gctggatgag gctgtgaata ccctgaagcc ctggtggccg
     5941 ggattatttg acggtgacac gccccggctt ctggcctgcg gtatccggga cgtgttactg
     6001 gaagacgtgg cgcagcggaa tatcccgctc tcgcataaaa aactgcgcag ggcgctgaag
     6061 gccatcaccc gttcagaaag ctatctgtgt gccatgaaag ccggtgcctg ccggtatgac
     6121 acggaagggt atgtgacgga gcatatttct caggaggagg aagcgtatgc gggagcgcgt
     6181 ctggcgaaaa ttcgtcgcca gaaccggata aaggcagaac ttcaggccgt gcttgatgag
     6241 aaataaaaaa agcctccccc agagagagga ggcagggaaa taaggcttaa aaggaaataa
     6301 cttccgcttc cgaaaggaag ccagtccact gaattattta tcacaataat actgacaact
     6361 aaagggggac aagaaaatga atggattcag aaacagttca cggaacggcc aggtctggcg
     6421 ttaccagcgt gccggcgggc gggccgttat tctggaagtc agtggacgct ggatggaagc
     6481 ggcagaagca tggcgacggg ctgcctgtgt tgcgcctagg acagactggc agcagtttgc
     6541 ccggaaaagg gctgaacact gccaccggcg ctgccgggga agggtgtaac gaaaaaagca
     6601 gccttcatta ctctaggagt cagaaggctg aaaggagttt atgagatttg cttcttaata
     6661 tccggtcggg cagtatgacc ggacagttct gagtagcaca gagtcttttc cgcattaagt
     6721 cgatttttta tattttgtga ttagtttcac aaaataaggt gttattcaat gtgtgctgca
     6781 ctattcaggg gggctgaaat acaggtggca tttatctgat atggagaata acatgaggaa
     6841 atatattcca ctggcattat ttatcttttc atggccggta ttgtctgcag atattcatgg
     6901 acgggttgtt cgtgtactgg acggtgatac catcgaggtc atggactcac tgaaggcggt
     6961 gcggatcagg ctggtaaata ttgatgcacc ggaaaagaaa caggattatg gccggtggtc
     7021 aacagacatg atgaaatcac tggtcgcagg aaaaacggtt accgtcaca
//
LOCUS       DAZPCS010000178         6921 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZPCS010000178 DAZPCS010000000
VERSION     DAZPCS010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6921
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.181"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..694
                     /locus_tag="ACHUQ7_003515"
     CDS             <1..694
                     /locus_tag="ACHUQ7_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFR1136766.1"
                     /translation="DAEAICEAASRPSMRFVQPRTETQQAIRALHRVRESLIRDKVKT
                     TNQIHGFLLEFGISLPTGDAVIKRLSLVLAEHEIPEYLSRLLVRLHTHYLYLVEQIAE
                     LESELSQSINADDTAQRIMTIPGVGPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQ
                     YSTGGKSTLLGISKRGDKNLRRLLVQCARSFMMQLERQHGKLAEWVREQLNKKHSNVV
                     ACALANKLARIA"
     gene            complement(1033..1803)
                     /locus_tag="ACHUQ7_003516"
     CDS             complement(1033..1803)
                     /locus_tag="ACHUQ7_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1136767.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQCNPNYPGWISIYVR
                     LDAPRLATLLVNRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVRDAVCSVSCRGAEDARRIRAALTTSGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(1848..2334)
                     /locus_tag="ACHUQ7_003517"
                     /pseudo
     CDS             complement(1848..2334)
                     /locus_tag="ACHUQ7_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
     gene            complement(2294..2515)
                     /locus_tag="ACHUQ7_003518"
     CDS             complement(2294..2515)
                     /locus_tag="ACHUQ7_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1136768.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            complement(2516..3199)
                     /locus_tag="ACHUQ7_003519"
     CDS             complement(2516..3199)
                     /locus_tag="ACHUQ7_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFR1136769.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(3263..3574)
                     /locus_tag="ACHUQ7_003520"
     CDS             complement(3263..3574)
                     /locus_tag="ACHUQ7_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1136770.1"
                     /translation="MKYDGDGRATARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLVSVTVYASPSPFSDERPSSRFRGITLLSKRRRLRYSTVGLTRYRK
                     R"
     gene            complement(3571..4473)
                     /locus_tag="ACHUQ7_003521"
     CDS             complement(3571..4473)
                     /locus_tag="ACHUQ7_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFR1136771.1"
                     /translation="MYFPSEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDARETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVVPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVNVLWGELEWSSP
                     TDDDEQPEVTGPAWIVDNVAHYGG"
     gene            4913..5871
                     /locus_tag="ACHUQ7_003522"
                     /pseudo
     CDS             4913..5871
                     /locus_tag="ACHUQ7_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
     gene            5871..6266
                     /locus_tag="ACHUQ7_003523"
     CDS             5871..6266
                     /locus_tag="ACHUQ7_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFR1136772.1"
                     /translation="MESSDPKKRKKVVAYLHPALYPQDNLTQQTIDSLPVQMRGDFYR
                     QSLICGAALYSVAPRLLTLISVFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIE
                     ASSENKSESITSKDDFEEQTRRNLSMLKK"
     gene            6449..6829
                     /locus_tag="ACHUQ7_003524"
     CDS             6449..6829
                     /locus_tag="ACHUQ7_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFR1136773.1"
                     /translation="MTMATINARIDDDIKNQADEVLKLMNISQTQAIAAFYQYITEQK
                     KLPFVITSIVKTPHDLLRESTDMLAEALAVISNLQVWTEQQDGIGKAKLMEYYRRLDA
                     LYCCAKEKIGLLSDNRDAELGCVP"
BASE COUNT         1891 a         1790 c         1676 g         1564 t
ORIGIN      
        1 tgatgcagaa gccatatgtg aagcggcttc acgaccatca atgcgttttg tccagccaag
       61 aactgagacc cagcaagcaa taagggcatt acaccgggtc agagaatcgc tcatcagaga
      121 taaggtgaaa acaaccaacc agatccacgg tttcctgctg gaatttggaa tcagcctgcc
      181 gacaggggat gccgtaataa aacggttgtc tctcgtattg gctgaacacg aaatccccga
      241 atatctgagc cgccttttgg tgaggttgca tacccactac ctttaccttg ttgagcaaat
      301 agcagagctg gaatcagaat taagtcagtc aatcaacgct gatgataccg ctcagcgcat
      361 tatgacaatt cctggtgttg ggccgattac tgccagctta ctatcgtcgc aacttggtga
      421 cgggaaacag ttttcatgca gcagagattt tgcggcttcc acagggctcg ttccccggca
      481 gtatagcact ggaggaaaat cgactctgtt gggtatcagt aagcgaggtg ataaaaattt
      541 acgccggtta ctggttcagt gtgcccgctc attcatgatg cagcttgaac gccagcatgg
      601 aaaacttgct gagtgggttc gggagcaact caacaagaag cattcgaatg tggttgcctg
      661 cgctctggca aataaactgg cccgaattgc ctaggcaatc actactcagc aaaaagaata
      721 tcaggcctga tatatagatg ctgacaaaac tcaattagct tattgcaaac aataagatac
      781 aagttaccgt ctggttttgc gaagattgat tattgatgac atgaacggca aatcgacctg
      841 attaaaaacc taacagggga aaatggctca ctgaagccga ggggattttt aggattatca
      901 ggtgcggaac tcatcgaggc gcgggcatcc cttgcccaca gagacgccgg atagatttaa
      961 gcaagcccac cattaatcaa aaatcagtgt tgcaaaaacg ggggtgacca tagattttcc
     1021 ccgccatcgt catcatttcc ggatttttgc cagggcatcc gccatgctgc ggatttcggc
     1081 ccgggcttcc cggacaactt cccgcagaac accacggtca aaccaccggc ggggcggttc
     1141 atccggtata cgcagcacat cgcaggccag cccactggac aggatatgct caatacaatc
     1201 gctgaccaga tacatttcca ccgccgaaaa acgtgcgccc cggttcagga tggcatcgag
     1261 caccacgggc aggttttcat cgtcttcatc gagccagcag gggtgatcgc tttccttcac
     1321 gaccaggcga aagcggcgcg tctggcgggt cagcaacgtc tgaccgcttg tggtcagcgc
     1381 cgcccggata cgccgcgcat cttctgcccc ccggcaactg acactgcata ctgcatcgcg
     1441 caccgcatca agaactgccc tgatttcgtc ttcggtcagc cattcaccgg catacgggaa
     1501 agacacctgc gttccgtccg ccggaagtac cggcagcgac tgccactgac tgccggacag
     1561 aacaagttcc gctccggttc cggtcagttt ctgaatggcg gaggccaggt gcggcggcag
     1621 cacgccatcg tggcggttaa cgagtaacgt cgccagccgg ggcgcatcca gccgcacgta
     1681 aatgctgatc cagcccggat agttcgggtt acattggtca acatccgtct cttccggcca
     1741 gccctgcgcc agcagcaggt tacgggcgtg acgacaaatc agagcattca gcgtttcatt
     1801 cataattcat gaactccctg tatcgccgcc cacaggcggc gataacatca gtcgttttcg
     1861 tgtgtgttgt ttttcagcag gctgcgggcc tgatcaacca tcgcctgtcg gcgggtaacg
     1921 tattccggga gggggaaacc gtgatcccgg gcagcccatt cacgtttcca cagtgcctgt
     1981 tcagcgatct gagtcagggt ctccagcagt tcagcaggga cggtcacctg gcggttttcg
     2041 cgcacgttgc tgataatctc catcgccgcg gcggaagaaa taccggactc aatccgctag
     2101 tcttccggga tgtcctccag gtgcgccagg acggtcctga tttcatgatc ggacagggaa
     2161 atatccattc cgtcagcgag ggcctgaatc tcttccgggg accagataac cagcatcagc
     2221 ggcgtgtctc ccgaatactt tgctgccagc gcccggcata gcgtttcgca tgttccgtac
     2281 atactctctc ccctcaatct ttgaagcagg aatccagttc cagaatttcg gtatatgcct
     2341 ccatcaagag gcggtttgcg cgaaacaacg tctgacgcac cagacgttcc ggcagggtgt
     2401 cagcattaaa gctgtaccgc cgctccatct cattcatggt tgtacgcagg tcgcacatgg
     2461 cattggccac ctccgccact tcggcgcgaa gtttttcgtg tccggcatag ttcatttacg
     2521 cagcctccgg cataaaccag tcatcattcg cggccccctg ctgcatggcc cgctgtacgg
     2581 cagccagtcg ttgctgtccg gcacggtgat actgctcaag cagctcgata ccgatatacc
     2641 ggcgtccgga ctggagggcg gcgacgcagg ttgaaccgct gcctgcaaac gggtccagca
     2701 ctatcgcatt cgggtgtgtg aagctctcaa tcagcggttg caggctggta acgggctttt
     2761 ccgtcgggtg atggcggttg cccgaatatt tccagcccaa cacgtccggc agcgggtttt
     2821 gtggcagacg tggacggcct tttgccagga tgtaggcgca ttcgtggcga tagcccacat
     2881 atgcggcctt cgatgtgtag tttttggtga agaccaggtg accaacaacg ctgaatcccg
     2941 catttttcca ggcagacata aagcgatcga cgcggttcca gccatagaag ctcaccatca
     3001 gcgcgtcttt tttgagtacg cggtacattt cattacaggc cggttgcagc cattcatcag
     3061 ttttatcacc agcaatggta cgcccctgac ggtcacgaaa accgacgaga tatggcggat
     3121 cggtgaggat gaaatcaatg gcgttatcag gaatacgggc cataacatcg atgcagttac
     3181 cgaggacaaa acgggacata acagatcctt ctcttacaga gggcacccct gtggtgcccg
     3241 ttccccgctg cctgtgggct gttcatcgct tccggtaacg ggtcaggccg accgtggaat
     3301 accgcagccg tcgtcttttt gacagcaagg ttatgccgcg aaagcggctt gaagggcgtt
     3361 catcagaaaa gggcgacggg ctggcgtaca ctgtcaccga caccagccgg gagcggtaaa
     3421 agcgcccgtc ccgtgcccgc cggacgcggg acactgacca caggcagcgt ttatgccgcg
     3481 ccgcgtcagc cggcgcagta aaaagaggag cacggcgaca tcctttgtcg gaaaagaaac
     3541 gggcggttgc ccgcccgtct ccgtcatact tcatccgcca taatgcgcca cattgtcgac
     3601 tatccacgca ggtccggtga cttccggctg ctcatcgtca tctgtcgggg aagaccattc
     3661 aagttccccc cacagcacat tgacgagctc tccacgctca tacaactgcc agccacagaa
     3721 atcacagccc tgttcggcat accagtgctc cagcgtgcag ctgaaacggc ggcttaattc
     3781 tgcaataacg tcgctttccg gctgacacca cggagtgtcg aaatccacct gaatgaagtt
     3841 ttctccctga ctgctgatat tcaggtcata tccacaaggc cacttcacgc cataccgttc
     3901 cgtataccag tgatatgcag aaggaacacc gttcagaaca ccaccattaa agccgttcac
     3961 ttcaacatcc aggcgggtcg gcagaaccgt cagcaggtca aacggcatgg ttttttccgg
     4021 cactacgttg tcacacagac ggttccacca caccgataca tcctcgttac cccatatgtc
     4081 acaccagtca cctctttttg cggtgaaatg cacagtgatg gtttccctgg catcatccgg
     4141 taatccttcc caacgacgct ggccagtacc gctctgtaac cagagttcat gcagcatcag
     4201 gcagttttgt tcatccagca acacaccgtt ctgcaggtgt gtcagccagc gggtgaacgc
     4261 gatattttcc gtcgatacag caccacgtcc ggcagccgtc actccggggc actgttcaga
     4321 gcggatattt tcagtgatct gcagaagtcc ggcacttccg gccagaaaca gctgaatacc
     4381 ttcatttgtg gcgcggcgat aaagcggtgt gactgcaccg ctggccagtc gtttaatctc
     4441 agcgatctgt gccggctcac taggaaaata catacgattc gagcaccagt ttggcatggg
     4501 aattctcctc tcacatgcgg cctccatcag gccgtgccgc acctgtaacc gggcgaccgc
     4561 atacgaatgc aggtgcggcc ccgaagccgc agcgcgtgga ggaggaatga cgaaacgtga
     4621 ccgggtgagg aggccggcga cagcgacaca cttgcatcgg atgcagcccg gttaacgtgc
     4681 cggcacggcc tggttaaata gtatccgtct cagttcgtgg cgcaaaatga gaaaccaata
     4741 agaactcaaa atgaaccaca acaagaacat aaagagactc taaaagagca acaccgtttg
     4801 acatagactc aaaatgagtc acaataagaa acaataaaaa actcaaaaag atgcaccgac
     4861 ataaaggatg ctcaatttga gataaacaca aactcaaaac gaggcaaaaa gaatgctaaa
     4921 agtatcatgc gatgatggtt caactaacgt aaaactggcc tggcttgaag atggcgaggt
     4981 aagaaccagt ctgtcaggaa acagctttaa agaaggctgg aacccgggac tgtttaatgc
     5041 cggcaaagtt tacaattacg tcgtggatga aaaaaataca cctatgattt gggaagcact
     5101 gctgtcatag gtacaacaca tgtcagttat caatacagca ccaccaatct gcttgccata
     5161 catcatgcac tgctgacatc cggccttcag ccacaggatg tagaactgac agtaacatta
     5221 cctgtgaccg aattctttga taatgacaat caaccaaatg aagaaagaat agaaagaaaa
     5281 aaggcaaatg ttctccgtga gatatctctg aataaagggg aaacatttaa aattaaaaaa
     5341 gttaatgtaa tgcctgaatc tttgccagct gcatttgaat cactaaaaaa agataaagta
     5401 aacaaacttg aacgcagcct gattatcgat cttggaggaa ccactctgga ttgtggttta
     5461 attctcggtg catttgaagg catttctgaa atccgtggct attcagaaat cgggacatcc
     5521 cgcatcactc ataccgtcat gaatgcgctg acgaaagcct ccacaccatg taattatttt
     5581 attgctgatg agttaataaa aaacagacac gataatgaat atctccagac attaataaat
     5641 gatgtagctg aaataaaaaa catttctcat gtaatagaca gagaagtgaa atctctggca
     5701 gaaagcatca ggcaggaaat ttcaacattc tcaggaatga accgaatcta tctcactggc
     5761 ggaggagccg agcttattta ccctcacata aagcaatatt tcccgaatct gaaagtgaac
     5821 aaggtggatg aacctcagtt tgcattggtg aaagcaatgg tgcatgcgta atggaaagca
     5881 gtgatccaaa aaaaagaaag aaagttgtgg catacctgca cccggcactt tatccgcagg
     5941 acaacctgac acaacaaaca atagactctc tcccagtaca aatgcgggga gatttctaca
     6001 gacaatccct tatttgcgga gcagctcttt attctgttgc cccccgactg ttaacgctaa
     6061 tcagcgtttt tttcagtgag aaaatcactg cagaaaatct ggtgaaactt atagaacaaa
     6121 caacaggata cacatccaca tcaattgata taagtgtgtt aaaaaacatc atagaggcat
     6181 cttcagaaaa caaatcagag agcatcactt caaaagatga tttcgaagaa caaaccagac
     6241 gcaatctttc tatgttaaaa aaataataaa agaagagcag gttcagtcaa ttacctgctc
     6301 ttcttttata catactaaaa aatcagattt ttacgaatat tcagattatc ataaaaaatg
     6361 caacagacag gaatgcaata attttgcatt atctcgttgc ttgtaatgca aatatgcatt
     6421 attgtcagtg tgtgacaata acagagagat gacgatggcg accattaacg cacgtattga
     6481 tgatgatata aagaaccaag cagatgaagt gctgaagctg atgaatatca gtcaaactca
     6541 ggctattgca gctttttatc agtatattac tgaacaaaag aaactgccat ttgtgatcac
     6601 ctcaattgta aaaacgccgc atgatttatt acgtgaaagt acagacatgt tagctgaagc
     6661 tcttgcagtg atttccaatc tacaggtgtg gactgaacag caggatggga ttggaaaagc
     6721 aaagctaatg gagtattacc gccgcctcga cgcactgtac tgttgtgcaa aagagaaaat
     6781 aggtcttctt tcagataacc gggatgctga actaggctgt gtcccttaat catctgagtt
     6841 atagtaactg tctgtttaca caacatattg aactatggct cgctacgacc ttcccgatga
     6901 ggcatggacc atcatcaagc c
//
LOCUS       DAZPCS010000179         6904 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZPCS010000179 DAZPCS010000000
VERSION     DAZPCS010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6904
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.182"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..380
                     /locus_tag="ACHUQ7_003525"
     CDS             <1..380
                     /locus_tag="ACHUQ7_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1136774.1"
                     /translation="FCIIVIFLSINYIAIVNVFKSSAVAQSTKYYEENDIHDGSNDIF
                     FMKKYGPPAHEFVRPRYKFVGEYYIGLNQTYRITDPEFQDVPIKEMFWNLQGDLNLTC
                     WFHYKDGQWRVITYIFWPPGARF"
     gene            405..2618
                     /locus_tag="ACHUQ7_003526"
     CDS             405..2618
                     /locus_tag="ACHUQ7_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFR1136775.1"
                     /translation="MSFVSTGNKPVGNGGPVITTPPIAGESGGMSTGSAVTDVSGAAE
                     EMAEQAAADLFGALPEPSGLVKAAVAAAQAAAAAAGISDMAGAVQDAAASLAAGAPGA
                     HNVTVSGSAVPPQMLLFAGMNGSEGLGNLFSYTVQLKTLDALNLGYVSPAANLPLQLM
                     VGKDLCVSIELDGGGKRYISGLVTAARVVGHESRSVTYELRIEPWLKLLTHTSDYKAF
                     QNKTVVDILDEVLDEYSFPVEKRLVENYPPRAWQVQYGETDFDFIQRLMQEWGIYWWF
                     EHSENSHTLVLVDAINGHKACPDSPLVEWHQEGLKLDKEFIHTITASERLRTGKWVMD
                     DFDFMKPRSLLKSTVASPRDTGHAEYEHYEWPGDYFTTGEGEMLTRIRMEAQRSPGSR
                     AHGAGHIRTLMTGYTFTLMNHPTAEINQEYLLVQTTLFLRDNAQHSGQDQHFTYVTTF
                     ELHPTSEVYRPQRTLSKPHTKGPQSAIVTGPAGQEIWTDKYGRVKVQFGWDRYGKNDE
                     NSSCWIRVSYPWAGKGFGMIQIPRIGQEVLVDFKNGDPDLPIIVGRTYNQDTMPPWGL
                     PGMASQSGIFSHSLQGGPTNGNMLRFDDKTGAEEVKFHAEKDLNTTVKNNETHTVMVD
                     RIKTIVKNETISVGEDRNATITKNDGLSVKLAQTINVGTTYRLDVGDQFTLRCGNAAL
                     VLHKDGSIEFCGKQLLLHTSDVMQLIGSGIDMNPDGGTAMTADDIAPLPTPDVSE"
     gene            2641..3086
                     /locus_tag="ACHUQ7_003527"
                     /pseudo
     CDS             2641..3086
                     /locus_tag="ACHUQ7_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233848.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
     gene            3108..6666
                     /locus_tag="ACHUQ7_003528"
                     /pseudo
     CDS             3108..6666
                     /locus_tag="ACHUQ7_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459289.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
BASE COUNT         1699 a         1694 c         2062 g         1449 t
ORIGIN      
        1 ttttctgtat tatagtgatt ttcctttcaa taaattacat agctatagtg aatgttttca
       61 agtcttctgc agttgctcaa tccaccaaat attatgaaga aaatgatata catgatggaa
      121 gtaatgacat cttttttatg aagaaatatg ggccgcctgc tcatgagttc gtgaggccac
      181 gatataaatt tgttggtgag tattatatcg gcttgaatca aacttatcgc ataacagatc
      241 ctgaatttca agatgttcct attaaggaaa tgttctggaa tttgcagggg gatttgaatc
      301 tgacatgttg gtttcattat aaagacgggc aatggagagt gattacttat attttttggc
      361 caccaggggc tagattttaa tccggtatta aaggagcaaa tatcatgagt tttgtatcta
      421 cgggtaataa accagttgga aacggtggac ccgtcatcac cacaccacca atagctggcg
      481 aaagtggcgg tatgagcaca ggttcagctg taaccgatgt atctggtgcc gccgaggaga
      541 tggcagaaca ggctgcggcg gacctgtttg gtgcgctgcc ggaaccgtca ggtctggtta
      601 aagccgcagt agctgctgcg caggctgccg ctgcagcagc agggatctcg gatatggctg
      661 gtgcagttca ggatgcagcg gcgtcgcttg cagccggtgc tccgggggcg cataacgtca
      721 cagtaagtgg tagtgcggtg ccgccgcaga tgcttttgtt tgcaggcatg aatggttcgg
      781 aagggcttgg gaatctgttt tcttacacag tacagcttaa aacgctggac gccctgaacc
      841 tggggtacgt ttcccccgct gctaacctgc cgcttcagct catggtgggc aaagacctgt
      901 gcgtcagtat cgaactggac ggcggcggaa aacggtatat cagcgggctg gtgacggcgg
      961 cgagggtggt cggccatgag agccgttcgg taacgtatga attacgcatt gagccatggc
     1021 tgaagctgct gacccatacc agcgactaca aagcgttcca gaacaaaacc gtggtggata
     1081 ttctcgatga agtactggat gaatattcct ttccggtgga aaagcggctg gtggaaaatt
     1141 atccaccccg cgcctggcag gtgcagtatg gtgaaaccga cttcgatttc atccagcgcc
     1201 tgatgcagga atgggggatt tactggtggt ttgagcacag cgagaacagc cacacgctgg
     1261 tgctggtgga tgccatcaat ggtcataaag cgtgccctga ctcgccgctg gtggagtggc
     1321 accaggaagg gctgaaactg gataaggaat ttatccacac cattacggcc agtgagaggc
     1381 tgcgtactgg aaagtgggtg atggacgatt ttgattttat gaagcctcgt tcgctgctta
     1441 aaagtaccgt ggccagtccg cgtgataccg gtcatgctga atacgagcat tacgaatggc
     1501 ctggtgatta cttcactacg ggcgaaggcg agatgctgac gcgcattcgt atggaagcgc
     1561 agcgcagccc aggcagccgg gcacatgggg ccggtcatat ccgtaccctc atgaccgggt
     1621 acacctttac gttgatgaat cacccgacgg cagaaattaa tcaggaatat ctgctggtac
     1681 agaccactct gtttttacgg gataatgcgc agcacagcgg tcaggatcag cattttacct
     1741 atgtcaccac gtttgaactg caccccacca gcgaagttta ccgcccacaa cgaaccctga
     1801 gcaaacccca caccaaaggc ccgcagagcg ccatcgtcac cggcccggcg gggcaggaaa
     1861 tctggacgga taaatatggc cgggtgaagg tgcagtttgg ctgggaccgt tacggaaaga
     1921 atgacgaaaa cagttcctgc tggatccgcg tcagctatcc gtgggcgggt aaaggattcg
     1981 gcatgatcca gatcccgcgt attggccagg aagtactggt ggatttcaaa aacggcgatc
     2041 cggatttgcc catcatcgtg gggcgcacct ataaccagga caccatgccg ccgtgggggc
     2101 tgccgggcat ggcgtcgcag agtgggatat tcagccactc attgcaggga gggccgacta
     2161 acggaaatat gctgcgcttc gatgataaaa cgggtgcgga agaggtgaag ttccatgcgg
     2221 aaaaagacct caataccacg gtaaaaaaca atgaaaccca cacggtcatg gtcgatcgca
     2281 taaaaaccat tgtcaaaaat gaaaccatca gcgtgggaga agaccgtaat gccaccatca
     2341 caaaaaatga tggtctttcc gtcaaactgg ctcaaaccat caatgtcgga acgacatatc
     2401 gtctggatgt tggcgatcaa tttacccttc gttgcggcaa tgcagcgctt gtattacaca
     2461 aggatggctc gattgagttt tgcggaaaac aactgttact gcacaccagc gatgttatgc
     2521 aactcattgg cagtggcatt gatatgaatc cggatggcgg cacagccatg actgcggatg
     2581 atattgcccc gttacctacc cccgacgttt ctgaataaaa aatataaaag gaatttttct
     2641 atgaccagcc tttaccgtat tcaggagggc tgctttgccc tgcctgaaac ttttcttgat
     2701 cgcaccgtca atatcttcgt cccatcgggc aatgaaaggg caactcccag tctgaatatt
     2761 ttccgcgata cgctcaggcc agatgaaaat ctgaccacct atattgaccg ccagatagcc
     2821 ttgatgaaaa aaaaaaccta ggccagcacc gggtactttc gcggggaacg gcactggcgg
     2881 gaactggcga agctgccatg acaggtgaac agattctcgc aactcatcgt tctggaaaaa
     2941 cagaagtgta ccagcgtcag gccgggttta tcaccgggcc agcaaaggta ctaatgttga
     3001 cgctgaccac tcagcgtcct tttgatgatc acaccgacca gctatggact gcatggttga
     3061 ctagttttca gcctgctaaa agctaaaaaa aacagggagg ttttgtcatg tatgaagccg
     3121 cacgggtgga tgaccccatc taccacacca gcgcccttgc cgggtttttg ataggggcca
     3181 tcataggtat cgccattatt gccgtcgcca gctttgcttt ttttacctgc ggctttttgg
     3241 ccgggctgat tttgagtttt ctggctgatc agatagcttc cggagtcctg caactgggtg
     3301 aggctattgg gcgctcgata cattccacag tgggaaaaat tctgaccggt tctgataatg
     3361 tcagcaccaa cagtcgcccg gccgcccggg ccgtactgag tacggtgata tgcgaagacc
     3421 attccccgga aatccgcatt gcccaaggtt ccggcaacat ctacatcaac agccagctgc
     3481 ccgtaaggat gaccacactg agtgtgatgc agtgattgag gacggctccc ccaatgtctt
     3541 tctcggcggc ggtacgcaga cggtactgga aatcagcccg gaaatccctg actggctgcg
     3601 tcaggtggtg gatgtgctgt atgtggtcgc cggaatgctt ggcgggctgg ctggcgcctg
     3661 gcgtcaggcg gcaaagatgg ggtcgaaatt tggtacaaaa tgtgcggcaa aatttattgg
     3721 tggcgaaatg gtcgggatgg ccatctctga cacagtcatg ggattgttca gcaatccggt
     3781 agatgtcacc actgggcaaa aaattttgct gccggagacg gattttaccc tgccaggacg
     3841 cctgcctgtc acctgttcgc gcttttacgc cagccacctg gaaactgaag gattgctggg
     3901 cagaggatgg cggctgaact gggagataaa cctgcgtgag gatgaaacat atatcacctt
     3961 catcggtgta cagggtcggg aactgagtta tccgaaagag atgctgatac cgggccacca
     4021 gatattcgac ccggaagagc agttttacct gagccgtctg catgacggat gctatgtgct
     4081 gcattacacc gactgcagct attacgtgtt tgatgaattt gatgaccacg gcgtcgcgcc
     4141 gttacttttt atggagacgc cgtaccggca gcgtattgct ttcgggcgtg agaacggcag
     4201 gctggtgcgg gtggcctcca gcagcggaca ccatctgtta ttgcaccgca cgatgacgca
     4261 agcaggggaa cgcctgtcgc atattgaact tttgaagggc gggcgtcccg gtaacctggt
     4321 ggaatatcgt tacgacgata acgggcaact gactggcgtg gtgaaccggg ctggtgtgac
     4381 ggtgcgccag tttgcctatg aaaatggcct gatgacagag catcgcaacg cgacgggctt
     4441 tacctgtacc taccactggg aagagattga aggtttcccg cgcgtggtgg aacacacgac
     4501 cagcgacggc gaggattacc ggttccacta tgattttgct ggcgggcaga cggtggtaac
     4561 cggcaggccg gagcagaagt ggcagtggtg gtttgatgaa gagacgtatg tgactgcgca
     4621 ccggacgccg ggcggcggaa tgtaccgctt cacgtacaac gaaaaccatt ttccggtagc
     4681 ggtggaactc cccggcgaac gcagggtaac gctggaatac gacaccctga gccgggtggt
     4741 gaaagagacg gacccggcag gccgggtgac acagacgcag tggaacggca gttttgctga
     4801 aatcacgcgc cgggcgctgg atgacgacca cgtatggaag gcggattaca acgaacacgg
     4861 ccaggtgata cgggagacgg acccggaagg gcgcgtcacg cggtacgggt atgatgatca
     4921 ggggctgccg gagacggtct gccaccccgg taaacagcag gaccgttaca cctggaacgc
     4981 gctggggtta ctgagcagcc accgtcgcat cacgggcagt gtgcagtcct ggcagtatac
     5041 gcagcgcggg atgctggcgc gccatacgga tgaagagaaa cgggagacgc gctggcagta
     5101 caccccggaa gggctggttg ccagtctgag caacggcaac ggggctcagt accggttcag
     5161 ctacgacggc gacggacggc tgacgggaga acagcgcccg gacggcctga taaggatgtt
     5221 tgcgctgaat gcggacgggt ttccggtcat catcccgacg cagggcacag agggcggggt
     5281 gcgcaatgaa cagcaggagc gtgatgcgct gggccgcctg ctgcgcagtg acacgcagca
     5341 cagtacacgg accttcagct acaaccgtct tgaccagata acggaagtga cgctcacccc
     5401 cacggaagaa ggggaacggc tgcaccacat gcaggcggac acggtgcgtt ttgcgtatga
     5461 ccgcagcggg tggctgacgg cagagcacag cgtgcacggc agtatcaaat accggcgcga
     5521 tgcgctgggc aatcccaccg atatcacgtt gccggacggg cagcatctca gtcatcttta
     5581 ctacggcagc gggcatctgc tccagacggc actggacggt atcacggtca gtgaatatga
     5641 gcgtgacagc ctgcaccgtc aggtgatacg cacgcagggg aaactggcga cgttcagcgg
     5701 ctacaacgcc gacaaccggt taagctggca gcgcagcctg ccaggtggca gccagaatcc
     5761 acagcaggcg gtactccccc ggcgcagaac gacagcgtga cggcgcggga ctatatctgg
     5821 aacgcagacg gtgaagtcgg cggcatcaat gacaaactgc gcggttgcct ggtgttcagc
     5881 tatgaccgca gcggctggct gaccagccgc acggggcaga tgtatgacca tgaccattat
     5941 tattacgaca aagcgggcaa cctgctgacg gatgagtatc agggagcggt gatggataac
     6001 cgcctgccgg gttacgggcg tgaccgctac cgctataacg aatggggcga actgacagag
     6061 cgcagggacc agcagctgga gtggaacgcg cagggccagt taacgcgggt catcagcagc
     6121 aacagcgaga cgcggtatca gtacgatgcg ctgggcaggc gcatcagcaa ggcgaccagc
     6181 aatctccata ccgaccgggg agaacgaagc cggacaacgt ttgtgtggga agggttccgg
     6241 ctgttacagg agacaacgtg gcagggaaag cggacatacc tgtacgatgc ggaacagccg
     6301 tacacgccgg tggctgccat cacggggcgc ggagagagcc agaaaatctg gtattaccac
     6361 acggacctga cgggcacggt acacgaggta accgcgccgg acgggacgct ggtgtgggcg
     6421 ggctaccagg cgggatttgg tgaaaaccgg ggcgacatca gcaacagcgg ggcgtacttt
     6481 gagcagccgc agcggctgcc ggggcagtac tttgacgaag aaacagggct gcattacaat
     6541 ctgttcaggt attatgcgcc ggagtgcggg aggttcgtaa gccaggaccc gataggatta
     6601 aacggtgggt tgaacttata tgcttatgcg cctaatccgt taggttggat cgatccgcta
     6661 ggactgaatt ctttaggaat agattcaaat aatatattta gaggagatgg taactataaa
     6721 ggtggcggaa ttggacagcc aaagagtggc acaatatcag cacaggacat tattgatcat
     6781 ctctaagaaa aaggtgatgg gcgtttaaca tcttttagta ctaaaaaaac atctagttcc
     6841 aactctcgtg gtgcagattt tttctcaaaa aaacatgtct tcaaagtggc agtttctgat
     6901 ctcg
//
LOCUS       DAZPCS010000180         6792 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZPCS010000180 DAZPCS010000000
VERSION     DAZPCS010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6792
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.183"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(185..670)
                     /gene="sixA"
                     /locus_tag="ACHUQ7_003529"
     CDS             complement(185..670)
                     /gene="sixA"
                     /locus_tag="ACHUQ7_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFR1136776.1"
                     /translation="MQVFIMRHGDAALDSASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(873..3017)
                     /gene="fadJ"
                     /locus_tag="ACHUQ7_003530"
     CDS             complement(873..3017)
                     /gene="fadJ"
                     /locus_tag="ACHUQ7_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFR1136777.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(3017..4327)
                     /gene="fadI"
                     /locus_tag="ACHUQ7_003531"
     CDS             complement(3017..4327)
                     /gene="fadI"
                     /locus_tag="ACHUQ7_003531"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFR1136778.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPTVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAYVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4507..4791)
                     /locus_tag="ACHUQ7_003532"
     CDS             complement(4507..4791)
                     /locus_tag="ACHUQ7_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFR1136779.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKSRSVKSEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5163..6503
                     /gene="fadL"
                     /locus_tag="ACHUQ7_003533"
     CDS             5163..6503
                     /gene="fadL"
                     /locus_tag="ACHUQ7_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFR1136780.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
BASE COUNT         1691 a         1818 c         1615 g         1668 t
ORIGIN      
        1 tttcaacatg taactttggg caaaatcaag atacgcgtag aagaataatt tccgaatgaa
       61 tgtagaaagt gttttattta tagataagga gagtcttcga tagtagtgag gttgacagat
      121 atccgctgcc taatgagcaa ttatgcccaa tgaatctacc tcattgggca taatttggaa
      181 cacatcagat agcttttgcc atcttcagat tacacgggct catctgccag ttgaatgtac
      241 cgtttccgct ttcatcaagc gttacgctgg caatggccga ggtggtaaac ataggcggcg
      301 tttcgcccgg acataactcg gcaaccagat acccgactaa cggtaagtga gagatcacca
      361 gtaccgaagc aacaccttca ttggtcaacg cctgcaaata ggcactgacc agaccaacat
      421 cgccgcaggg cgttaactcc ggcagaactt ctgcactgga aggcaggttc agacaatccc
      481 ctacttcttc cagtgtttgc tcggctcgca ggaacgggct caccagaacg cgttcgattt
      541 ccactttttg acctttcagc cagttcgcca tcaggcgaga ttcgtcacaa ccattagtgg
      601 tcagaggacg aaccgaatca ctggcggaat cgagggctgc gtcgccgtga cgcatgataa
      661 aaacttgcat attgcaccgc ttttgttaac cagattcgcc cgttttcttt catctgaaac
      721 cagagaaaga aaacggtggc cggcattgtg ccttatccat tcaccgaatg aaacgctgtt
      781 ttttacctca atggcgtaag tatagtcaat atgcgtttac attttgacca acactccgcc
      841 attcagcgcg gattcatata gctttgacct tcttattgca ggtcagttgc agttgttttc
      901 caaaaacttt ccccacgcgc gcccatctcg accaaacgct cgcaaggggt aaaacgggaa
      961 ccatactgcg tggccagtcg ttgcattatt gcaaccactt cgcccgcgcc gagagaatcg
     1021 atatagcgga acggtccacc gagaaatggc ggaaaaccaa tgccaaatac cgcaccaata
     1081 tccccgtcac gcacgctacg gataacctgc tcatcaacac aacgtactgc ttcattcagc
     1141 atcaacatca cacaccgttc agcaacctgc ggtgcggaga gtcgcccctg cccttgtgcg
     1201 ccaatgagcg ggtaaatggc aggatcgacc tgttttttgc ttttacgccc tttctgacca
     1261 taaagataga aaccccgacc attttttctg cctttgcgat cgtcgttcaa aattgaagaa
     1321 acaacatttg caggcgcgct aaaacgttct ccataagcgg cttccagtac aggaataatt
     1381 ttagtcccgg tgtcgattcc tacctcatcc aaaagttgga ttgggcctac cggaaaacca
     1441 aatttcacta gcgcggcatc aatgtgctct acccgttcac cttcggtcag catgcggata
     1501 gcttcattaa tgtaaggcgc taagatgcga ttgacgtaaa acccggcttt gtcacgcacg
     1561 acaatgggcg ttttgccctg ttttttcgcc agttttaccg tagtggcgat ggtttgcgcc
     1621 gatgtacccg catgaggaat aatctctacc agcggcattt tttccaccgg actgaagaaa
     1681 tgcaggccaa taacttgctc aggtcgcgcg gcgtgagcgg cgatatcacc aatcggtaaa
     1741 gatgacgtgt tcgaagcaaa aatggtatga gcggcgcaat tttgctccac ttccgccacc
     1801 atctgttgtt ttaattcgag attttcaaac accgcttcaa taatcagatc gcgatgggca
     1861 aagccgcgat agtccgtcgt tccggagatt aatgccagct gtttgtcacg ttcgctggct
     1921 ttgagatgac gacggcgaac tttgccctcc agctgatccc aactgtactt cagcgcatga
     1981 tttatgccct gcgggttgat atctttaatt ctgaccggaa gccccgcttt acaagcagtg
     2041 acataagcaa taccgccgcc catcaagcca ccacctaaaa ttcccacgct gtttaatggc
     2101 gcaggcggtg catcactacc gggatctttc ttcacgtccg tactggcaaa aaagatacta
     2161 cgcaaagctt gcgattgcgg cgtcatcgcc agttcgccaa aggcgcgagc ttcagcgtca
     2221 tagccgctgc tggtgccctg cgctaatccc gtttcaacaa cctccaggat gcgttctgtc
     2281 gccggataat tgccttgagt tttgtgttct gttttcttgc cgaccatttt gaacagcagc
     2341 gcacgaccta acggccccgc cagaatacgc tcgcgtacag gtagagggcg ggaagatggg
     2401 cgatccttct tcgccagctc aacagcggct tccagcaaaa tggagtgcgg aacaacgtca
     2461 tccaccagcc ccagctttaa tgcctgtttc gcccgaagtt gttttccggt gagaatcatc
     2521 tctaatgctg tactgacgcc tatcagacgc ggtaaacgct gggtgccgcc tgaaccgggt
     2581 aacaatccaa gttgtacttc aggcaaaccg agcaccgttt taggatcgtc agtacaaacg
     2641 cgaccgtggc acgccagcgc cagctccagc ccaccgccca ggcaagcgcc atgaatagcc
     2701 gcgataaccg gaatgggcaa agcatgaatc tccgccatca actgttggcc ctgccgcgcc
     2761 agtgcttccg cttcttgcgc cgttttgcag ttgccaatca tgttgatgtc tgctccggcg
     2821 ataaaattgt ccggtttcgc ggagataaac accacgccgc gcaactcttt gttttcacgg
     2881 agttgcttaa taatggcgcg cacctgcgag gcaaattccg ccttcagggt attcattttc
     2941 tcatccggta cgtcgatggt gatgatagca atgttgtcca gacgaacatt aagagtaaac
     3001 gctgatgcca tttccattat tccgcctcca gaaccattgc cgcgccaagc ccaccggcag
     3061 cacaggcggt aactaaacca aatccaccgc cgcgacggcg aagttcatgc aatgtctggg
     3121 taatcatccg cgcgccggtc gccgcgaagg gatgtccgta agcaatcgaa ccgccaagca
     3181 cgttaaattt gctatcgtcc acttcgccag tggcatgtgc acgccccagt acttcacgag
     3241 caaaacgttc actacccagc aactgaatat tcgccagtgt ctgagctgca aaggcttcgt
     3301 gcatatcgat caatgtcagt tcgctcatcg tcaaaccggc acgttccagc gccagcggtg
     3361 ttgaccaggc tggaccgagc aacatgtcct gccagacatc aatcgcagta aatgcgtagc
     3421 tgcgcagata ccccagcggc accagcccta attctttcgc ccgggattca gtcatcagga
     3481 tcaccgctgc cgcgccatcg gtcagcggcg tactgtttgc cgccgttacc gttccgtgtt
     3541 tgcgatcaaa cgccgggcgc agctttgcgt aatcggcaag cgaggaatta ccgcgaatat
     3601 tgttgtcttc gacaagcggt tgtttataag gagggataaa ggcagtcatc acctcttctt
     3661 tgagttttcc gtctgaccat gcctgagcgg cacgctgatg cgaacggtgc gctaatgcat
     3721 cttgctgttc tcgggtgatg ccgtaggttt tcgccatttg ttcggcggtg tcgcccatcc
     3781 gcaagccagt agaatattct gctaccgcag gcggtacggg cattaagtcg cgcaaacgca
     3841 ggcgagagaa gagtttcagg cgctggctca tggttcgagc tttgttgaca tcaaccagca
     3901 cgtacgccag ttttttactg acgccaattg gcaatacgga agaggaatct gccccaccgg
     3961 caatccccgc tcggatagtt cccgccatca ggctttctgc gacgtttgca accgcttgga
     4021 aactggtagc gcaagcgcgg ctgacgctgt aagcatcggt atgcacattc attccggtac
     4081 cgagaacaat ttcacgcgca atgttggggg cttcaggcat ttgtacgacc tgaccaaaga
     4141 ccagctgttc aatcacttca gcggggatct cgctgcgtgc cagcagttcg cctaccacca
     4201 tcttccctaa atcaaccgtg ggaatgccat gaaaagccgt cgcctggcgg gcaaaaggcg
     4261 tacgtaaacc gctaacaatg gcgatacgat cgccctggcg ggtaaccagt ggtaaaacct
     4321 gacccataac actcccctgt aaaaaaataa ataaagtggt ctgacctgat catagtctta
     4381 accatttttt tacatttagc caagcggaga aaaaggaaag tgggagctat gacacagtga
     4441 agacagaaga aagaaaagaa aacgcccctg ccagcaactg acaggggcgt acgtacccga
     4501 gaggaattaa cgcagaccca gctggaagat cagcatttca gcttcgcagg caaaagtgaa
     4561 atcgatatcc aggcgcacac cgtcagactc ttcagtgaaa gtcggggaga ttttgcacgg
     4621 ttcggatttc acgctacggg atttttcagt cagcgccgcc agcgtttgtt ctgcttctgc
     4681 gcggtttgca aacacgcggc tgtaagacgc ggtgcagtcg gagttgtcca taatggtgcc
     4741 aacatccata cagcagcaaa ccggggtttc atcagcacta catttactca tcgttgattt
     4801 cctctgtatg tgcacccaag gtgccagata aacgttgtgg atattttacg cttccggaaa
     4861 gtgctgctcc agttgttaat tctgcaaaat cggataagtg accgaaatca cacttaaaaa
     4921 tgatctaaaa caaaattcac ccgaatccgc gagtgcgcaa cctccaaatt ttgccagctg
     4981 gatcgcgttt cttagatcat atttgaaaaa agatggaaac atacttgcaa cattctagct
     5041 ggtccgacct atactctcgc cactggtctg atttctaaga tgtacctcag accctacact
     5101 tcgcgctcct gttacagcac gtaacatagt ttgtataaaa ataaatcatt gaggttatgg
     5161 tcatgagcca gaaaaccctg tttacaaagt ctgctctcgc agtcgcagtg gcacttatct
     5221 ccacccaggc ctggtcggca ggctttcagt taaacgaatt ttcttcctct ggcctgggcc
     5281 gggcttattc aggggaaggc gcaattgccg atgatgcagg taacgttagc cgtaaccccg
     5341 cattgattac catgtttgac cgcccgacat tttctgcggg tgcggtttat attgacccgg
     5401 atgtgaatat cagcggaacg tctccgtctg gtcgtagcct gaaagccgat aacatcgcgc
     5461 ctacggcatg ggttccgaac atgcactttg ttgcaccgat taacgaccaa tttggttggg
     5521 gcgcttctat tacctctaac tatggtctgg caacagagtt taatgatact tatgcaggcg
     5581 gctctgtcgg gggtacaact gaccttgaaa ccatgaacct gaacttaagc ggtgcgtatc
     5641 gcttaaataa tgcatggagc tttggtcttg gtttcaacgc catctacgct cgcgcgaaaa
     5701 ttgaacgttt cgcaggcgat ctggggcagt tggttgctgg tcagattatg caatctcctg
     5761 ccgggcaaac tcctcaaggg caagcattgg cagctaccgc caacggtatc gacagtaata
     5821 ccaaaatcgc tcatctgaac ggtaaccagt ggggctttgg ctggaacgcc ggaatcctgt
     5881 atgaactgga taaaaataac cgctatgcac tgacctaccg ttctgaagtg aaaattgact
     5941 tcaaaggtaa ctacagcagc gatcttaatc gtgcgtttaa taactacggt ttgccaattc
     6001 ctaccgcgac aggtggcgca acgcaatcgg gttatctgac gctgaacctg cctgaaatgt
     6061 gggaagtgtc aggttataac cgtgttgatc cacagtgggc gattcactat agcctggctt
     6121 acaccagctg gagtcagttc cagcagctga aagcgacctc aaccagtggc gacacgctgt
     6181 tccagaaaca tgaaggcttt aaagatgctt accgcatcgc gttgggtacc acttattact
     6241 acgatgataa ctggaccttc cgtaccggta tcgcctttga tgacagccca gttccggctc
     6301 agaatcgttc tatctccatt ccggaccagg accgtttctg gctgagtgca ggtacgactt
     6361 acgcatttaa taaagatgct tcagtcgacg ttggtgtttc ttatatgcac ggtcagagcg
     6421 tgaaaattaa cgaaggccca taccagttcg agtctgaagg taaagcctgg ctgttcggta
     6481 ctaactttaa ctacgcgttc tgataacgcg ttcgcctgga taaagtcacc tgctatgcag
     6541 gtgactttaa ctcccccact tcaccgaaag tagtttcctc attgcttgcc tcctgagttt
     6601 tgcaaacagc ctgatggcag cttgcccttt caaatcagta agtggtatcc acagacaaac
     6661 ctgaagggaa atgcatattt tcaagcgttc tgctcgtcct tctcaaagag tttacttttc
     6721 tgcatttcca ggatactcaa cccccctggc tattgtgcgc ttatacactc aaattaaaga
     6781 taggttctaa at
//
LOCUS       DAZPCS010000181         6743 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZPCS010000181 DAZPCS010000000
VERSION     DAZPCS010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6743
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.184"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            242..2158
                     /gene="dnaK"
                     /locus_tag="ACHUQ7_003534"
     CDS             242..2158
                     /gene="dnaK"
                     /locus_tag="ACHUQ7_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFR1136781.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTIPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            2247..3377
                     /gene="dnaJ"
                     /locus_tag="ACHUQ7_003535"
     CDS             2247..3377
                     /gene="dnaJ"
                     /locus_tag="ACHUQ7_003535"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFR1136782.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(3481..3633)
                     /gene="mokC"
                     /locus_tag="ACHUQ7_003536"
     CDS             complement(3481..3633)
                     /gene="mokC"
                     /locus_tag="ACHUQ7_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFR1136783.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            4219..5385
                     /gene="nhaA"
                     /locus_tag="ACHUQ7_003537"
     CDS             4219..5385
                     /gene="nhaA"
                     /locus_tag="ACHUQ7_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFR1136784.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLVLKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            5451..6350
                     /gene="nhaR"
                     /locus_tag="ACHUQ7_003538"
     CDS             5451..6350
                     /gene="nhaR"
                     /locus_tag="ACHUQ7_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFR1136785.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(6388..>6743)
                     /locus_tag="ACHUQ7_003539"
     CDS             complement(6388..>6743)
                     /locus_tag="ACHUQ7_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFR1136786.1"
                     /translation="YQFHHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILKN
                     ELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVS
                     SGQIMSRVKIVVMYN"
BASE COUNT         1706 a         1593 c         1749 g         1695 t
ORIGIN      
        1 tatatgaata aaattgctgt caattttacg tcttgtcctg ccatatcgcg aaatttctgc
       61 gcaaaagcac aaaaaatttt tgcatctccc ccttgatgac gtgggttacg accccattta
      121 gtagtcaacc gcagtgagtg agtctgcaaa aaaatgaaat tgggcagttg aaaccagacg
      181 tttcgcccct attacagact cacaaccaca tgatgaccga atatatagtg gagacgttta
      241 gatgggtaaa ataattggta tcgacctggg tactaccaac tcttgtgtag cgattatgga
      301 tggcaccatt cctcgcgtgc tggagaacgc cgaaggcgat cgcaccacgc cttctatcat
      361 tgcctatacc caggatggtg aaactctggt tggtcagccg gctaaacgtc aggcagtgac
      421 gaacccgcaa aacaccctgt ttgcgattaa acgcctgatt ggccgccgct tccaggacga
      481 agaagtacag cgtgatgttt ccatcatgcc gttcaaaatt attgctgctg ataacggcga
      541 cgcatgggtc gaagttaaag gccagaaaat ggcaccgccg cagatttctg ctgaagtgct
      601 gaaaaaaatg aagaaaaccg ctgaagatta cctgggtgaa ccggtaactg aagctgttat
      661 caccgtaccg gcatacttta acgatgctca gcgtcaggca accaaagacg caggccgtat
      721 cgctggtctg gaagtaaaac gtatcatcaa cgaaccgacc gcagctgcgc tggcttacgg
      781 tctggacaaa ggcactggca accgtactat cgcggtttat gacctggggg gtggtacttt
      841 cgatatttct attatcgaaa tcgacgaagt tgacggcgaa aaaaccttcg aagttctggc
      901 aaccaacggt gatacccacc tgggtggtga agacttcgac agccgtctga tcaactatct
      961 ggttgaagaa ttcaagaaag atcagggcat tgacctgcgc aacgatccgc tggcaatgca
     1021 gcgcctgaaa gaagcggcag aaaaagcgaa aatcgaactg tcttccgctc agcagaccga
     1081 cgttaacctg ccatacatca ctgcagacgc gaccggtccg aaacacatga acatcaaagt
     1141 gactcgtgcg aaactggaaa gcctggttga agatctggta aaccgttcca ttgagccgct
     1201 gaaagttgca ctgcaggatg ctggcctgtc cgtatctgat atcgacgacg ttatcctcgt
     1261 tggtggtcag actcgtatgc caatggttca gaagaaagtt gctgagttct ttggtaaaga
     1321 gccgcgtaaa gacgttaacc cggacgaagc tgtagcaatc ggtgctgctg ttcagggtgg
     1381 tgttctgact ggtgacgtaa aagacgtact gctgctggac gttaccccgc tgtctctggg
     1441 tatcgaaacc atgggcggtg tgatgacgac gctgatcgcg aaaaacacca ctatcccgac
     1501 caagcacagc caggtgttct ctaccgctga agacaaccag tctgcggtaa ccatccatgt
     1561 gctgcagggt gaacgtaaac gtgcggctga taacaaatct ctgggtcagt tcaacctaga
     1621 tggtatcaac ccggcaccgc gcggcatgcc gcagatcgaa gttaccttcg atatcgatgc
     1681 tgacggtatc ctgcacgttt ccgcgaaaga taaaaacagc ggtaaagagc agaagatcac
     1741 catcaaggct tcttctggtc tgaacgaaga tgaaatccag aaaatggtac gcgacgcaga
     1801 agctaacgcc gaagctgacc gtaagtttga agagctggta cagactcgca accagggcga
     1861 ccatctgctg cacagcaccc gtaagcaggt tgaagaagca ggcgacaaac tgccggctga
     1921 cgacaaaact gctatcgagt ctgcgctgac tgcactggaa actgctctga aaggtgaaga
     1981 caaagccgct atcgaagcga aaatgcagga actggcacag gtttcccaga aactgatgga
     2041 aatcgcccag cagcaacatg cccagcagca gactgccggt gctgatgctt ctgcaaacaa
     2101 cgcgaaagat gacgatgttg tcgacgctga atttgaagaa gtcaaagaca aaaaataatc
     2161 gccctataaa cgggtaatta tactgacacg ggcgaagggg aatttcctct ccgcccgtgc
     2221 attcatctag gggcaattta aaaaagatgg ctaagcaaga ttattacgag attttaggcg
     2281 tttccaaaac agcggaagag cgtgaaatca gaaaggccta caaacgcctg gccatgaaat
     2341 accacccgga ccgtaaccag ggtgacaaag aggccgaggc gaaatttaaa gagatcaagg
     2401 aagcttatga agttctgacc gactcgcaaa aacgtgcggc atacgatcag tatggtcatg
     2461 ctgcgtttga gcaaggaggc atgggcggcg gtggttttgg cggcggcgca gacttcagcg
     2521 atatttttgg tgacgttttc ggcgatattt ttggcggcgg acgtggtcgt caacgtgcgg
     2581 cgcgcggtgc tgatttacgc tataacatgg agctcaccct cgaagaagct gtacgtggcg
     2641 tgaccaaaga gatccgcatt ccgactctgg aagagtgtga cgtttgccac ggtagcggtg
     2701 caaaaccagg tacacagccg cagacctgtc cgacctgtca tggttctggt caggtgcaga
     2761 tgcgccaggg attcttcgct gtacagcaga cctgtccaca ctgtcagggc cgcggtacgc
     2821 tgatcaaaga tccgtgcaac aaatgtcatg gtcatggtcg tgttgagcgc agcaaaacgc
     2881 tgtccgttaa aatcccggca ggggtggaca ctggagaccg catccgtctt gcgggcgaag
     2941 gtgaagcggg cgagcatggc gcaccggcag gcgatctgta cgttcaggtt caggttaaac
     3001 agcacccgat tttcgagcgt gaaggcaaca acctgtattg cgaagtcccg atcaacttcg
     3061 ctatggcggc gctgggtggc gaaatcgaag taccgaccct tgatggtcgc gtcaaactga
     3121 aagtgcctgg cgaaacccag accggtaagc tattccgtat gcgcggtaaa ggcgtcaagt
     3181 ctgtccgcgg tggcgcacag ggtgatttgc tgtgccgcgt tgtcgtcgaa acaccggtag
     3241 gcctgaacga aaagcagaaa cagctgctgc aagagctgca agaaagcttc ggtggcccaa
     3301 ccggcgagca caacagcccg cgctcaaaga gcttctttga tggtgtgaag aagttttttg
     3361 acgacctgac ccgctaacct ccccaaaagc ctgcccgtgg gcaggcctgg gtaaaaatag
     3421 ggtgcgttga agatatgcga gcacctgtaa agtggcgggg atcactcccc gccgttgctc
     3481 ttactcggat tcgtaagccg tgaaaacagc aacctccgtc tggccagttc ggatgtgaac
     3541 ctcacagagg tcttttctcg ttaccagcgc cgccactacg gcggtgatac agatgacgat
     3601 cagggcgaca atcatcgcct tatgctgctt cattgctctc ttctccttga ccttacggtc
     3661 agtaagaggc actctacatg tgttcagcat ataggaggcc tcgggttgat ggtaaaatat
     3721 cactcggggc ttttctctat ctgccgttca gctaatgcct gagacagaca gcctcaagca
     3781 cccgccgcta ttatatcgct ctctttaacc cattttgttt tatcgattct aatcctgaag
     3841 acgcctcgca tttttgtggc gtaatttttt aatgatttaa ttatttaact ttaatttatc
     3901 tcttcatcgc aattattgac gacaagctgg attatttttg aaatattggc ctaacaagca
     3961 tcgccgactg acaacaaatt aattattact tttcctaatt aatccctcag gaatcctcac
     4021 cttaagctat gattatctgg gcttagggtc actcgtgagc gcttacagcc gtcaaaaacg
     4081 catctcaccg ctgatggcgc aaattcttca atggctcgta aaaaacgaat tattcctaca
     4141 ctataatctg attttaacga tgattcgtgc ggggtaaaat agtaaaaacg atctattcac
     4201 ctgaaagaga aataaaaagt gaaacatctg catcgattct ttagcagtga tgcctcggga
     4261 ggcattattc ttatcattgc cgctatcctg gcgatgatta tggccaacag cggcgcaacc
     4321 agtggatggt atcacgactt tctggagacg ccggttcagc tccgggttgg ttcactcgaa
     4381 atcaacaaaa acatgctgtt atggataaat gatgcgctga tggcggtatt tttcctgtta
     4441 gtcggtctgg aagttaaacg tgaactgatg caaggatcgc tagccagctt acgccaggcc
     4501 gcatttccag ttatcgccgc tattggtggg atgattgtgc cggcattact ctatctggct
     4561 tttaactatg ccgatccgat tacccgcgaa gggtgggcga tcccggcggc tactgacatt
     4621 gcttttgcac ttggtgtact ggcgctgttg ggaagtcgtg ttccgttagt gctgaagatc
     4681 tttttgatgg ctctggctat tatcgacgat cttggggcca tcattatcat cgcattgttc
     4741 tacactaatg acttatcgat ggcctccctt ggcgtcgcgg ctgtagcaat tgcggtactc
     4801 gcggtattga atctgtgtgg tgtacgccgc acgggcgtct atattcttgt tggcgtggtg
     4861 ttgtggactg cggtgttgaa atcgggggtt cacgcaactc tggcgggggt aattgtcggc
     4921 ttctttattc ctttgaaaga gaagcatggg cgttctccag cgaagcgact ggagcatgtg
     4981 ttgcacccgt gggtggcgta tctgattttg ccgctgtttg catttgctaa tgctggcgtt
     5041 tcactgcaag gcgtcacgct ggatggcttg acctccattc tgccattggg gatcatcgct
     5101 ggcttgctga ttggcaaacc gctggggatt agtctgttct gctggttggc gctgcgtttg
     5161 aaactggcgc atctgcctga gggaacgact tatcagcaaa ttatggtggt ggggatcctg
     5221 tgcggtatcg gttttactat gtctatcttt attgccagcc tggcctttgg tagcgtagat
     5281 ccagaactga ttaactgggc gaaactcggt atcctggtcg gttctatctc ttcggcggta
     5341 attggataca gctggttacg cgttcgtttg cgtccatcag tttgacagga cggtttaccg
     5401 gggagccata aacggctccc ttttcattgt tatcagggag agaaatgagc atgtctcata
     5461 tcaattacaa ccacttgtat tacttctggc atgtctataa agaaggttcc gtggttggcg
     5521 cagcggaggc gctttattta actccacaaa ccattaccgg acagattcga gcgctggaag
     5581 agcgcctgca aggcaaatta tttaaacgca agggacgtgg tctcgaaccc agcgagctgg
     5641 gagaactggt ctatcgctat gccgataaaa tgttcacctt aagccaggaa atgctggata
     5701 ttgtgaacta tcgcaaagaa tccaatttat tgtttgacgt tggcgtggct gatgcacttt
     5761 ccaaacgcct ggtcagtagc gtacttaacg ccgcagtggt agaaggcgag cccattcatc
     5821 ttcgctgctt cgaatccacc cacgaaatgc tgctggagca attaagtcag cataaactgg
     5881 atatgatcat ttctgactgt ccgatagact ctacgcagca ggaaggcctg ttctccgtga
     5941 gaattggcga atgtggcgtg agtttctggt gtacaaatcc accaccagaa aaaccgttcc
     6001 cggcttgtct ggaagaacgg cgacttttga ttcctgggcg acgttcaatg ttagggcgca
     6061 aattgcttaa ctggtttaac tcccagggat taaacgtaga aatcctcggc gagtttgatg
     6121 atgccgcttt gatgaaagct tttggtgcga tgcacaatgc aatcttcgtt gccccaacgc
     6181 tttatgcata tgacttttat gccgataaaa ctgtcgtaga aattggtcgc gtcgagaatg
     6241 tgatggaaga gtaccatgct atttttgctg agcggatgat tcagcacccg gcggtacagc
     6301 gaatctgcaa tacggattat tctgcgcttt ttagtccagc ggtgcgttaa tcggcagctc
     6361 ccccaaagtt aaggtggggg agatagatta gttgtacatt accacgattt tgactcggct
     6421 cattatttgc ccgcttgaga cattgtttcc atatgtacgc gggcgaataa atagaggaat
     6481 ctgattactt ccttcatggg gatgctgaaa agagtagtaa ttgcttttac catactggat
     6541 ttcattcgta ccatcattgg agagttgaaa gcctatttga cccgcaccgt cctcactgtt
     6601 ttctaactca ttttttaata tgctggttcc tgactcctgt atcgtcactg gttcaaagta
     6661 atattcaacg tcactgggtt caaaaactaa ttcaccttgc tcgccctgga tgcagtcaaa
     6721 tcttatatga tgaaactgat aat
//
LOCUS       DAZPCS010000182         6656 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZPCS010000182 DAZPCS010000000
VERSION     DAZPCS010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6656
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.185"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(16..1026)
                     /gene="pyrD"
                     /locus_tag="ACHUQ7_003540"
     CDS             complement(16..1026)
                     /gene="pyrD"
                     /locus_tag="ACHUQ7_003540"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFR1136787.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            complement(1137..1874)
                     /locus_tag="ACHUQ7_003541"
     CDS             complement(1137..1874)
                     /locus_tag="ACHUQ7_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFR1136788.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPALLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTNWCQ
                     VIAPCHVAWRTINDYGGLSAKKEQNLP"
     gene            complement(1840..2355)
                     /locus_tag="ACHUQ7_003542"
     CDS             complement(1840..2355)
                     /locus_tag="ACHUQ7_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1136789.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            complement(2363..2903)
                     /locus_tag="ACHUQ7_003543"
                     /pseudo
     CDS             complement(2363..2903)
                     /locus_tag="ACHUQ7_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            complement(2915..3984)
                     /locus_tag="ACHUQ7_003544"
                     /pseudo
     CDS             complement(2915..3984)
                     /locus_tag="ACHUQ7_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            complement(3975..6575)
                     /locus_tag="ACHUQ7_003545"
     CDS             complement(3975..6575)
                     /locus_tag="ACHUQ7_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFR1136790.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQVCVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQTTLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINS
                     AITRMDAICR"
BASE COUNT         1703 a         1717 c         1484 g         1752 t
ORIGIN      
        1 aattcgaaga aataattaga tatgggtaac gatttcttta atcagcggcg gacctttaaa
       61 aataaaacca gaataaattt gcaccagtga ggcacccgca gcaatctttt cacgcgcagc
      121 gataaccgag tcgatgccgc caacaccgat gatcggtaag cgaccgttta attctagtga
      181 caagcggcga ataatttctg tgctttttaa ctgaagcgga cgaccactta agccaccggt
      241 ttgatcgcaa tttttcattc cctgaaccag agaacgatcg agagtggtat tggttgcaat
      301 aacgccatca atattatggc gaactaaact atcggcaacc tggatcaatt cgtcttcaga
      361 aagatccggc gcgatcttca ctgcgatcgg cacatattta tggtgcatcg cttgcaaatc
      421 attttgctta tttttaatcg cggttaagag atcatccagc gcttcaccat attgcagcgt
      481 gcgtaatcct ggggtattcg gcgatgaaat attgatggcg atatatcccg cataggcata
      541 gattttttcc atacaaatca gatagtcatc tttgccctgc tccactggcg tatctttatt
      601 tttgccgata ttaataccca agacgccgtc ataatgggcc ttttttacgt tctctacgag
      661 gttatcaacg ccaagattat taaagcccat acggttgatc aaaccttcgg catctaccag
      721 acgaaagaga cgcggcttgt cattaccagg ctgtggacgt ggcgtgacgg taccgatctc
      781 gatcgatcca aatcccatcg cgcctaacgc atcaatgcac tccccgtctt tatcaagacc
      841 ggctgccaga ccaagcggat ttttaaacgt caggcccatg cagttaacag gtttcgcagg
      901 cactttctgc cgcaccagtg cttcaaacgg tgttccggta atgcggcgta attgctgaaa
      961 agtaaactca tgagcgcgct ctggatcgag ctggaaaagg gctttacgaa cgaaggggta
     1021 gtacatgaac tctcctggat tcccggtgtg caaaccgggg gcgtattatg tgcgagttcg
     1081 gaccaaaagg gaattgacct gcggcaaaaa aaacgcaaac gttttcagaa cctgtttcag
     1141 ggcagatttt gctccttctt tgcactcaac ccgccgtaat catttatcgt ccgccaggcg
     1201 acatgacacg gtgctattac ctgacaccag tttgtctgac gctggctttt aggcggcacg
     1261 actcccgcat tacttaacgc tttaccattt acgctcacct gaatcaggtt aatgtagtaa
     1321 ggcgttgggt tagtgagttg tacaccctgc gaattccgtg tccagcgtaa ttgttgataa
     1381 gcttccagcg ggtcgcccgg caaaccttcg ggtcgccaga acagtttaaa taccgagcgc
     1441 atcgccactt ttacgctctg attttcaact ttgccggatg gcacgctggc aatgcttaac
     1501 tcaaatagcg tttcgcgatc cacaggcaag atgtcgcttt ccgttctcag caagcgcaac
     1561 gtgcctgtag taccgggctt caggagaata agtggcggag cggccagtaa tagtgctggc
     1621 actgtcgacg cttccccccc ggcccaacgc gttgggcgat tgattttgct gttaataagc
     1681 caagattcct gactggtatt agtcagtaaa atagatatcg attctctgtc tgccggaaag
     1741 ataaatcgcg ttccgccgac caccactcct gcctgcaatt cacccgccac cagcagcgat
     1801 aaaacggcga aaatcaagag cgctaatctg ttccacgtat tagtcataat gaagtacaaa
     1861 tgtcgcgctg gcattcactt tacccgactg cacatcgcta ttgacaggtc gcaattgcgc
     1921 ataaaaagtt aatggcacct taccgctatg taggctgtag tctttactcg cctggttaag
     1981 cggaatcaat atcttcgcat catccagaat cgcaattgcc agcccactgg ctgcatccgt
     2041 accactttca atggacagca tttcgctctg cactccgtcg ggggttcctg aaaaggtgac
     2101 atcaaccgtg ctgacatctg ttccgcaatc ctgcaaatta atgatgaaag atttctccgg
     2161 gccataattc ccatgaaaaa aacgtgtcag cgagacatcg cctaactgca cattgatttg
     2221 cttgctatcc tgtgccagca cgcatgtatt acggcgaaac gatccgctaa tgttgaccgt
     2281 gccatcatgg gcaaatagct cacagccccc cgttaatccc agtaagaaca gaatccagat
     2341 tatccgtttc aacatcactc cttcactggt aaaccagatc aaaatagaga accgccgtcg
     2401 cattaccgcc cgttgctccc gcctttgtgg acttataacg aagttgatat ttgagtgtgt
     2461 tatccccggc ttttaagggc gtaagaggct ggacctgatt gagcgggatt tcttgctgac
     2521 tttgcgcatc aagaatgcac ggcaatcccc gttgccatac caccgcttcc gcctgtgtca
     2581 gttagtttga gcagtgtcgg cgcaagggtg tcagcctcgc cgctaaaaag gacgtccgcg
     2641 ccagtaatac cagcggcaca accggtgata tcaatattca aatcagcagc agtgcttacg
     2701 gtattcgcgg cctgaaaatc actgatatta aaatcaccaa tatgaatatt ttgtaatgca
     2761 cttttggtgg cgacatcaca gccctgatcg accacattcc cataaatatg catactgact
     2821 tgttcaggcc cagccatccc gacatggatg ttaagaaggc taaagaacaa aataacgcac
     2881 tgatatatcg tttttttctt catacgttca cgcgttaatc aaaatttatt ctcaccgttg
     2941 ccgtggcttc aaatcgcccg gtttctggtg gtacgcttgt cgtgctgact ggatagacat
     3001 tcattcgcgg atactgtctg catcatcagg aatagagagc ggtagtgtcc caccagcagg
     3061 ggaaataatg ttgtgctggc tatcggtcac caccacgccc acgccaggac gtgacgtctt
     3121 aatcgcctgg ggatagctgg ggtctgaggt tgccgtcaga ctcagattga cgttaactaa
     3181 ccctgcgttt cccgtgttac acctgaccgg aattgcaagt tctgccgttc tggccccaag
     3241 aggcttatgc cctacaacac gaaagttagc cgcaaatacg tcgcccagat caacctcgat
     3301 tgtttctccg gcattgacgg aacatgagcc aagtgaattg atcgtaccag acaggtaata
     3361 cactaaaacc ggtgtaccag tagtcgtgca ggaatcacct gcgggtatgt tgtagcattc
     3421 aaataatcgc gccagcgcca taggaggaat gacgaccgaa ccaacgaacg gctgcaatat
     3481 tttcagggaa aagctaccgc tattaccgct agccccctgg ccacctaaac gttgaccgcc
     3541 gggatcgcaa atatgccatc gtgtatctgc ccctgtgccc acgttttcga aaggaaccgc
     3601 agtaggagaa ctgcctttaa cctgcaataa aactttggcg gcaagatagt cattaatttt
     3661 gtaccagttg tcgcctaaat agactaacgg tgaatccgcg gcgaatgccc atcggccact
     3721 tctgacattg tctttatcgc aatcacagga aacataataa tcattcgatt cgttccactg
     3781 ataaaatttg tctttaacta ctccagccgt attttcttct tcatccagag ttttaacgcc
     3841 aaaattgacg ccaatatacg gattcccctg agtggtatga cactgcccga tataagcagc
     3901 agtttgagcg caccatagca ttaagacggc ggcaaaaaat agtcgtagta ataacacgca
     3961 tttttctcca aagattatct gcatatggca tccattcgcg taatcgcaga gttaatttgt
     4021 tgttcggtaa gctggtaact gatatgacaa atttgatcgg gagcttcgcc ccagcgtaca
     4081 gttatcgctc cctgagcggg caaaccggac atatagagcg agccatcatc atcaataatg
     4141 ccgctattag tctgtatgcc gtccagcgtc gctatcgcgc caaaacgcag cggtataccc
     4201 tgctttgatg ttttcattaa tacccttgct cccgcatggg cattgaactc cgccagaacc
     4261 aacgcccctt tggtgggaac cacgttgact actgcatttt caagatccac gttacgtttt
     4321 aacgatgccg catcaagtgc gattcgattt tcccgataag aagtggcata ggggacaacg
     4381 gtatatccgc gccagtcagt tttcagcccg gtgctgttcg ccacacgagt attctctgcg
     4441 cctggtgctg caatcagcac gttagtttcc cccagcgatt gccccagggt aatgccctgt
     4501 gaatgggcaa ctaaactgcc tgaaagagcg tagttcagtt gttgttgact gccgttatcg
     4561 ctgtagttgt agcccactcg ggcatctgca aacgcccctt tgtagtccat gctggcgcta
     4621 ccattagccg ttttcccctc gctgttatat ccctgttgca cgctgtagct caggttaccg
     4681 tcatcgagta gtgtctccgt taaacccagg ttttgtgtaa tgtgcccttc gttatcgata
     4741 ctggtgttag aggtcatcca ggcgtaattt tttttcctgg tatagctatc attacttctg
     4801 gggagcaaca gattgagcgg cagggaaaaa tttagtgcaa acacctgatc agcatcaggt
     4861 tgaccacggg acttactgta gttccaggaa acccccagcg agagatcttt aatttgcgtg
     4921 ttgtagccaa actgtaatag ccgatcctgt tgatcggtat gccagtaagt ttgctggcta
     4981 ccacttaaat aaaacgaacc gtactcgcct aattgctggg agatattgac ctgcagtttg
     5041 ccacgtttgg tgtaaaacaa attgtaataa cgcgaccaca tcggcgtatc ttcatcatca
     5101 ccgtcattaa attcgtagcc atccatatgt ttatacatgg tgtcggaaag ggtgtagaaa
     5161 cccgacgtcg aatagcgata gcccatcaat tgaaaattag tgcccaattg gttgagcgtt
     5221 ttggcataca gaaaacgata tgactgacct ttatgttcgc tttggtctgc cagcgtactc
     5281 ttcgcctggg tcgcatcgaa cgaaattgct ccgaaatcgc caaggttaaa tcccagacca
     5341 aacatggcgg cacggtaata ttcagcatat tgtccaccac cataccatgt cgttccccac
     5401 ggtccgcccc actgcaacgt ggtctgggca aatttgctct cctgctgttc attactattg
     5461 gttctgtatt tcgccagtgt caccgcgtat ttgattcgcc cctgacgctg gagtaatggc
     5521 acgctggaaa acggtacgct gtagctattg acgctgccgt ccgcttcttt gatttcaact
     5581 aacaaatcac cgctcgacga cgtggaatag agatcactaa tttcaaaagc accaggcgat
     5641 acataagttt ggtaaatggt gtaaccattt tgcttaatcg ttatctgggc attgcttttg
     5701 gcgataccac gcaccacagg cgcaaaccct ttcaggctgt ctggcagcat gttgtcatct
     5761 gacgccagct gcacaccacg aaagctgaca ctatcgaaaa aatcgcctgc agtataatca
     5821 tcacctacgg tcagttcgcc ttttagtgga ataaccgccc gctgcaaata gctgctgaga
     5881 ttcttccatt ctgcggtttg gccactactg cgactccatg ttgaattgtt gcgtaatctc
     5941 catgggccta aattaacgcc actgtttaaa ttcagaaaat agctgtcgcc agaatcactg
     6001 tctgcgctgc tatgaatact gttagccccg ctaaacgaat atcccagtaa tagcgcgttg
     6061 atcccttcat cccaacgttc aggggggaca taaccacggg cattactttt gatggcgatt
     6121 tgcggtactg aaatttccag acggagttta ttcacattaa acgttacgct ggcatccgga
     6181 ataatctccg ccaatggcac acacacttgt ttgtccagtt gtttaaaagc cggaaacgca
     6241 tcagtattaa cacccattgc atccaggctt tcagtcgtca aacaggcggc aatccccttc
     6301 tctggcgtta tctcaacaaa attgacgttg cgcgtatcta caattgtctg attcagaaca
     6361 atatcgacac gataaacccc tggcgttatt ttttgtcctt tttcgaaacg agacaaatcg
     6421 gccaccattg agggatcatc tttgaaaaaa gcaggatcga accaggtttc agcttgtgca
     6481 ttgaacgctg ccgacacaaa caccaccagc ccaccaataa acgatggcac tccaccagag
     6541 cttaacaggc tgtgttgtcg atgagttcta tacatctcgc tctcttaact gaagacggct
     6601 tacgatttac agttgagttg cggcgtttcc gccccgtagt cattgatggt tcgata
//
LOCUS       DAZPCS010000183         6597 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZPCS010000183 DAZPCS010000000
VERSION     DAZPCS010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6597
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.186"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..1076
                     /locus_tag="ACHUQ7_003546"
     CDS             <1..1076
                     /locus_tag="ACHUQ7_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GAPES1 domain-containing protein"
                     /protein_id="HFR1136791.1"
                     /translation="AEQSEVSPEQRRAICEAFESANNTHGLNLTAHKYPGLRGTLQTA
                     STDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDLNDRYVYFYEPV
                     NVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQNVMSLLTPVYVA
                     GQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQYV
                     SYVHDLPGGIRVSLSIDILYFITSSRKSVLFWILTALILLNMVRMHFRLYQNVSRENI
                     SDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDTLGHQEGDLAIT
                     LHDAYKASDERLYVNKQNKNSRS"
     gene            1119..1622
                     /gene="yeaK"
                     /locus_tag="ACHUQ7_003547"
     CDS             1119..1622
                     /gene="yeaK"
                     /locus_tag="ACHUQ7_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFR1136792.1"
                     /translation="MTEMAKGSVTHQQLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(1623..1727)
                     /gene="yoaI"
                     /locus_tag="ACHUQ7_003548"
     CDS             complement(1623..1727)
                     /gene="yoaI"
                     /locus_tag="ACHUQ7_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFR1136793.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERIG"
     gene            1897..2343
                     /locus_tag="ACHUQ7_003549"
     CDS             1897..2343
                     /locus_tag="ACHUQ7_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFR1136794.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(2300..3118)
                     /gene="nimR"
                     /locus_tag="ACHUQ7_003550"
                     /pseudo
     CDS             complement(2300..3118)
                     /gene="nimR"
                     /locus_tag="ACHUQ7_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
     gene            3215..4395
                     /gene="nimT"
                     /locus_tag="ACHUQ7_003551"
                     /pseudo
     CDS             3215..4395
                     /gene="nimT"
                     /locus_tag="ACHUQ7_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416305.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
     gene            4450..4797
                     /locus_tag="ACHUQ7_003552"
     CDS             4450..4797
                     /locus_tag="ACHUQ7_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFR1136795.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYRAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(4819..5073)
                     /gene="yoaF"
                     /locus_tag="ACHUQ7_003553"
     CDS             complement(4819..5073)
                     /gene="yoaF"
                     /locus_tag="ACHUQ7_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFR1136796.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            5256..6281
                     /gene="dgcP"
                     /locus_tag="ACHUQ7_003554"
     CDS             5256..6281
                     /gene="dgcP"
                     /locus_tag="ACHUQ7_003554"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFR1136797.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVLWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLHEANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            6314..6412
                     /locus_tag="ACHUQ7_003555"
     CDS             6314..6412
                     /locus_tag="ACHUQ7_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFR1136798.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGCYAAPGA"
     gene            6415..6489
                     /gene="yoaJ"
                     /locus_tag="ACHUQ7_003556"
     CDS             6415..6489
                     /gene="yoaJ"
                     /locus_tag="ACHUQ7_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFR1136799.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
BASE COUNT         1599 a         1599 c         1615 g         1784 t
ORIGIN      
        1 ctgctgagca gtcggaagtg tcgccagaac agcggcgcgc catctgcgag gcttttgagt
       61 ctgccaataa tacccatggc ttaaacctga ctgcccataa atacccgggc ttacgcggca
      121 cactacaaac cgcatccact gactgcgaca caattgtgga agctgcagca ctattacccg
      181 cttttgatca ggcagtggaa ggcaaccgcc accaggatga ttacggttca ggtcttggga
      241 tggccgaaga gaaatttcac tattatctcg atctcaatga ccgctatgtc tatttttatg
      301 agccggttaa tgttgaatac tttgcgatga ataactggtc cttcctgcag tcaggaagta
      361 ttggcatcga tcgcaaagat attgaaaagg tatttaccgg gcgtaccgta ttgtcgagca
      421 tttaccagga tcagcgtact aaacagaacg tgatgagttt gctgacgccg gtatatgtcg
      481 cagggcagct aaaagggatt gtgctgctgg atattaacaa aaacaatttg cggaatatct
      541 tttacactca tgaccgccct ctcctctggc gttttctcaa tgtcacgcta accgataccg
      601 attcggggcg cgacattatc atcaaccaga gcgaagataa tctgttccag tatgtcagtt
      661 acgtccatga cttacctggc ggcattcgtg tctcgttatc cattgatatt ctttacttta
      721 tcacgtcttc gaggaaaagc gttctgttct ggattttgac ggcgttaatt ttgctgaata
      781 tggtccggat gcacttccgt ttataccaaa atgtgtcgcg agaaaatatt agtgatgcga
      841 tgactggact gtataatcgc aaaattttaa cccctgaact ggagcagcgg ttgcagaaac
      901 tggtgcaatc cggttcttcg gtgatgttta ttgcaattga catggacaag ttaaagcaaa
      961 taaatgacac cctcggtcat caggaggggg atttagcgat tacgttacat gatgcgtata
     1021 aagcttccga tgagcgttta tatgtcaata agcagaacaa aaacagccgt tcatgataac
     1081 cttctgtggt tgtttgcttg taatctcagg agcgtgaaat gactgaaatg gctaaaggaa
     1141 gcgtgacgca tcagcagtta atcgctttat tatcacaaga aggtgctaac ttccgcgtcg
     1201 ttacccatga agcggtaggt aaatgtgaag cggtgtcaga aattcgtggt accgctctag
     1261 gccagggtgc taaggcactg gtatgtaaag tcaaaggcaa tggcgtaaat caacatgttc
     1321 tggcgatcct cgcagccgat caacaggccg atctgagcca actcgccagc catataggcg
     1381 gattacgcgc ttcgttggcc agcccggcgg aagttgatga actcacaggc tgtgtcttcg
     1441 gcgcgatccc ccctttcagc ttccatccaa aactcaaact ggttgccgac cctctactct
     1501 tcgaacgatt tgatgaaatc gccttcaatg cagggatgct ggataaatcc gttattttga
     1561 aaaccgcgga ttatttgcgc attgcgcaac cagaactggt caatttccgc cgcactgcgt
     1621 agctagccta tccgttcaat aagcagaacg gacaaaacca gcacaaccgc aatagcaaaa
     1681 aacgatgatg tgataatcag tgtctcgacg aacatttgat cgttcatagc atgcccccgg
     1741 gagatagctg ttttcacgtt attgttagcg tgcacaaatg gcagtttgat gacagttcgc
     1801 ctgatttttt atgcaaaaaa cgtaaagatt ttctacttcc ttcctgcagc aagcataaag
     1861 taagcaggct tattattttt tggcaaggaa accacgatgt ttgatgtcac tttgctgatc
     1921 ctgctcgggt tagctgcgct gggctttatc agtcataaca ccactgtcgc cgtttcaatt
     1981 ctggtgttga tcattgtccg cgtcacaccg ttaagcacct ttttcccgtg gattgaaaaa
     2041 caggggctaa gtatcgggat aatcattctg acgattggcg ttatggcccc catcgccagc
     2101 gggacgctac cgccctcaac cttgattcac tcgtttctga actggaaatc gctggtggca
     2161 attgcagtag gcgttattgt ctcctggctg ggtgggcgcg gcgtgacgtt aatgggcagc
     2221 cagccgcaac tggttgctgg gctgctggtg ggcactgttt taggcgtagc gctgtttcgc
     2281 ggcgtaccgg ttggcccgct tattgccgcg ggtctggttt cgctgattgt ggggaaacag
     2341 tagttaatcc cgcaatatat cggcctggcg tttgccccaa gcctttctta aacatagtaa
     2401 taaaggccgt tgttgaatca taccccagcg tatgagcaac tttctgtaca gtatcgcctt
     2461 tcactaatcc ctgaagcgcc ataatcagct gtaactgctg acgccactgg cgaaaactca
     2521 accccgtctc tttcactatc aggcgggcaa gattgcgttc actcatcgca aaaaagccag
     2581 cccattgccc caatgccccc cactcgacgg gccctttcgc catcatctcc accatcgtgc
     2641 ggattttagg atgagaagaa acgggcaggt gcagctgttg ttgtggctgc tgagggagtt
     2701 catcaaacag gacctgaatt aatctgcggg tcatcggctc agcacgctga gtcgtcgtcc
     2761 tgtttgccag ggttaaaatc aattcccggc ataagggcga tattttcaat gtgcaacatg
     2821 gtcggcattg tcactgcgct gggttcgata aacagaaaac agagttcagc gttagctgtc
     2881 acttgattgc tatgcttcac cccgcccggt atccaaaccg catattgtgg cggcaccatc
     2941 cacaaagcat tttccaccgt acaggtaatt gcaccatgta atgccagtat cagctgtcct
     3001 ttgcggtgct gatgtacagg actggttaac tcatccgttc cggcgtgaat acaaaatgcg
     3061 accgcggctt catggtgtcg gtgggttcat agccattgag attcaacctg tgcatcattt
     3121 tgtccgaact tagcgataat ttgtcatttt agcttgattc aacataacaa taaaaacggt
     3181 aaggtacagc ctcgtttgta acaatgagaa gcatatgacc tgttcaactt cattaagcgg
     3241 caaaaacagg attgtcctta tcgctggcat tctgatgatt gccacaacat tacgcgtcac
     3301 ctttaccggc gcagcgccgt tactggatac gattcgttcc gcttactcgc tgacgacagc
     3361 gcaaaccggc ttattaacca ccctgccatt attggccttt gcgctaatct cacctctggc
     3421 tgccccggtg gcgcgacgtt ttggtatgga acgtagcctg tttgccgcgt tacttttgat
     3481 ctgtgcaggt atcgcaattc gttctctccc ttcgccttac ttattatttg gcggtacagc
     3541 ggtcattggc ggtgggattg cattaggcaa tgtcttactg ccaggattaa ttaaacgcga
     3601 tttccctcat tccgtcgcca gacttaccgg cgcatattcc ctgacaatgg gagctgcagc
     3661 ggcactggga tcggctatgg tcgtgccgct ggctttgaac ggttttggct ggcaaggcgc
     3721 gttgttcatg ctgatgtgtt ttcctctgct ggctcttttt ttatggctgc cacagtggcg
     3781 aagtcagcaa catgcaaatt tgagtacctc acgcgcctta catactcggg gtatctggcg
     3841 ttcgccactt gcctggcagg taacattgtt tcttgggatc aactcactgg tctattacgt
     3901 gattattggc tggcttccgg cgatcctcat cagtcacggc tatagcgaag cacaggcggg
     3961 ttcactgcat ggtttgctgc aactagccac agcagctccc ggtttgctga tcgcactttt
     4021 cttacatcat gtgaaagacc agcgtggtat tgcagcgttc gttgccttga tgtgcgcagt
     4081 gggcgcggtt gggctctgct ttatgccagc gcacgcgatc acctggactc tgcttttcgg
     4141 ttttggttcc ggcgcaacaa tgatactggg gttgacgttc attggtcttc ggcaagttct
     4201 gcgcatcagg cggcggcact ctcggggatg gcacaatccg tcgggtattt gctggcagcc
     4261 tgtgggccgc cgctaatggg taaaatacac gatgctaacg gtaactggtc cgtaccactg
     4321 atgggtgttg caatactttc actactgatg gcgattttcg gactttgcgc cgggagagac
     4381 aaagaaattc gctaatatcc ggtgctatag tgacgtgaca aatcttgcgt gaaagggaga
     4441 acaaacacga tgaatattca gtgcaaacgc gtttatgatc cggctgaaca gagcgatggt
     4501 tatcgcgtac tggtcgaccg cctctggccg cgcggtatca aaaaaaccga tttagccctt
     4561 gatgagtggg ataaagaaat cacgccgtca acggaactgc gcaaagcctt tcacggcgaa
     4621 gtcgtcgatt ttgcaacctt tcgcgagcaa tatcgcgcag aactggcgca acacgagcaa
     4681 gaaggaaagc ggctggcgga catcgccaaa aaacagccgc tgaccctgct ctacgcagca
     4741 aaaaacacca cgcagaacca tgcgctggtg ctggccgact ggctacgtag cttgtgattt
     4801 tagtacggca tccggcggtt atttttcacc agccggatgg tcccgtcgcc acaatgccca
     4861 ttcatcaatc gtttcaccgc ccggtaattt gcaattgttg ctgacccctt gcgctgtctg
     4921 cactggaatg agcgtcccgc ccttctgctg gcaatagacc gacgccggat ttgccatacc
     4981 aatctgcggc ggtttaggtg cttctggctg agaaggggtt gaacaaccag ccaggaccag
     5041 caagcaaggc agaacaaaac tgataatttt catttattga tctcacatat ttatccaagc
     5101 ttagagtatc gcggtatcgt tttgttttgc agcactattt ttattacatt cactcaaaac
     5161 atattacgtc ttgtttcatc tttgttgatg atgttttatc atgcctgcaa agattaaata
     5221 atcagcactt acccgccgta tcctggagtt gttccgtgtc agatcagatt atcgcccgcg
     5281 tctcgcaatc ccttgccaaa gaacagtcac tggaaagtct ggtccgacag cttctggaga
     5341 tgctggaaat ggtcactgat atggaatcaa cctacctgac caaagtggat gtcgaagcgc
     5401 gcctgcagca tattatgttt gcccgtaaca gccagaaaat gcacatcccg gagaatttta
     5461 ccgtcttgtg ggattactcg ttatgcaaac gcgccattga tgaaaactgc tttttcagcg
     5521 atgaagtccc tgaccgttgg ggcgactgta ttgcggcacg caatcttggc atcaccacat
     5581 ttctgagcac gccaattcac ttaccggatg gatcattcta tggcacgctt tgcgccgcca
     5641 gcagtgagaa gcgccagtgg agtgaacgcg cggaacaggt tttacagtta ttcgccggac
     5701 tgattgcaca atatattcaa aaagaggcac tggttgaaca gctgcacgaa gccaatgctg
     5761 cgctgattgc gcaatcgtat accgactcgt taaccgggct accgaatcgg cgggcgattt
     5821 ttgaaaatct gacgacgctg ttttccctcg cccggcatct taaccataag ataatgatcg
     5881 cgtttatcga tctggataac ttcaaattaa tcaatgatcg ttttggtcat aatagtggcg
     5941 atctgtttct cattcaggtt ggcgagcgcc ttaatacgct ccagcaaaat ggcgaagtta
     6001 ttggtcgtct cggtggtgat gagtttttgg ttgtttcact aaacaacgag aatgcggata
     6061 tttcgtcgct gcgagaacgt attcaacagc aaatacgtgg agaatatcac ttaggtgatg
     6121 ttgatttgta ttatcccggt gccagtcttg gcatagtaga agtcgatcct gaaacaaccg
     6181 atgcagacag tgccctgcat gctgccgata ttgcgatgta tcaggagaaa aaacacaaac
     6241 agaaaacacc ttttgtcgcg catccagcgc tacattcctg aggcgtattc acatcctttt
     6301 gattggtgat aacatgcgaa tcggtattat ttttccggtt gtaatcttca ttacagcggt
     6361 cgtattttta gcatggtttt ttattggctg ctatgctgcc ccgggagcat aaagatgaaa
     6421 aaaacaacga ttattatgat gggtgtggcg attattgtcg tactcggcac tgagctggga
     6481 tggtggtaac gtcacctcta aaaaatagca aaggctgcct gtgtgcagcc tttgtgcaat
     6541 ttaagcgtta acttttaatc ttcctgtaga taaatagcac gacaatcgca ccaataa
//
LOCUS       DAZPCS010000184         6521 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZPCS010000184 DAZPCS010000000
VERSION     DAZPCS010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6521
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.187"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..115
                     /locus_tag="ACHUQ7_003557"
     CDS             <1..115
                     /locus_tag="ACHUQ7_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1136800.1"
                     /translation="LDEINNTYYNNDYYNQAIREEPIDFLDRSFSSNSWPF"
     gene            439..1827
                     /gene="narU"
                     /locus_tag="ACHUQ7_003558"
     CDS             439..1827
                     /gene="narU"
                     /locus_tag="ACHUQ7_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFR1136801.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     PVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAVTETAATLGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1909..5649
                     /gene="narZ"
                     /locus_tag="ACHUQ7_003559"
                     /pseudo
     CDS             1909..5649
                     /gene="narZ"
                     /locus_tag="ACHUQ7_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
     gene            5646..>6521
                     /gene="narH"
                     /locus_tag="ACHUQ7_003560"
     CDS             5646..>6521
                     /gene="narH"
                     /locus_tag="ACHUQ7_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFR1136802.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCNNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQQIDDYY
                     EPFTFDYEHLHSTPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRN
                     FEAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYD
                     ADRIEEASSTEREVDL"
BASE COUNT         1558 a         1714 c         1690 g         1559 t
ORIGIN      
        1 cttagatgaa ataaacaata cttactataa taacgattat tacaatcagg caataagaga
       61 ggaaccgata gactttctgg acagatcgtt ttcctccaat tcatggcctt tttagtgaat
      121 aaatcatcac taattctata agtgtctggt cttgttgaga atatagttaa tatgtaaccc
      181 tttagattca cgatctgaag ggttgaaaca tgacatacgt tcaaagatta acccccgttt
      241 gaaggttagc ttgttagtta catttagtaa cacatatctg gctacttaat ccccattctg
      301 cccccgcggt tacgctttct tatctattct ttcaatcagc acttcaccca attcatcctt
      361 aattctaata actggctatt gcgccttacc tcctgttttg tcttttttca ttaccaatgt
      421 gtgcatatga ggaacaatat ggcactgcaa aatgagaaaa atagtcgtta tcttttacgc
      481 gactggaaac cagaaaatcc ggccttctgg gaaaataaag gaaaacatat tgctcgaaga
      541 aacctctgga tatcagtcag ttgtctactt cttgccttct gtgtctggat gctatttagc
      601 gcagttaccg ttaatctcaa taaaatcggt tttaatttta ctaccgatca actcttttta
      661 ttaaccgcat taccctccgt ttctggcgca ttattgcgtg ttccctactc ctttatggtg
      721 cctatattcg gtggacgccg atggccggtt tttagtactg caatcctgat tattccttgc
      781 gtctggctcg gaattgccgt gcaaaatccg aatactcctt ttgggatatt tatcgttatc
      841 gctttgctat gcggttttgc aggtgcaaac tttgcttcga gcatgggcaa tatcagtttc
      901 ttctttccaa aagccaaaca agggagcgct cttgggatta atggcggatt aggaaactta
      961 ggtgtaagtg taatgcagct ggttgcaccg ctggtcattt ttgtacctgt atttgccttt
     1021 ctcggcgtca atggcgtacc gcaggccgac ggttcggtga tgtcgctggc gaatgccgca
     1081 tggatttggg taccgctact ggcgattgcc acgatcgccg cctggtcagg gatgaatgat
     1141 atcgccagtt cacgcgcctc aattgccgac cagctccctg tcttacaacg cctgcatctc
     1201 tggctgctga gcctgcttta ccttgccacc ttcggttcgt ttatcggttt ttctgcgggt
     1261 tttgccatgc tggcaaaaac ccagttcccg gatgtgaata ttctgcgcct ggcgttcttt
     1321 ggcccattta ttggtgccat cgcgcggtcg gttggtggtg ctatttccga taaattcggc
     1381 ggcgtgcggg tgacgttgat caactttatt tttatggcga ttttcagtgc cctgctgttc
     1441 cttaccttac cgggcacagg ctccggtaat ttcatcgcct tttacgccgt atttatgggg
     1501 ctgtttctga ccgcgggtct gggaagtggt tctactttcc agatgatcgc cgtcatcttt
     1561 cgccagataa ccatttatcg ggtgaagatg aaaggcggta gtgatgagca agctcagaga
     1621 gaagccgtca ccgaaacggc ggcaacgctg ggctttatct cagccattgg cgcagtgggc
     1681 ggctttttta ttccgcaggc atttggcatg tcgctcaata tgaccggctc tccggtcggc
     1741 gcgatgaaag tgtttttaat cttctacatc gtttgtgtgc tgctgacctg gctggtttat
     1801 ggtcggcgga agttcagcca aaaataagag atcgatgcct gatgtacaac catgtcgcat
     1861 ccgacaaggc atcagatccc aattcattcc tggagcagga gtcatgtcat gagtaaactt
     1921 ttggatcgct ttcgctactt caaacaaaag ggcgaaacct ttgccgatgg tcacggacag
     1981 gtgatgcata gcaaccgcga ctgggaggac agctatcgcc agcgttggca gttcgacaaa
     2041 atcgtgcgtt ccacccacgg cgttaactgt acaggctcct gtagctggaa aatctacgtt
     2101 aaaaatggtc tggtgacctg ggaaatccaa cagaccgact acccgcgcac tcgccctgac
     2161 ctgcccaatc atgaacctcg cggctgcccg cgtggcgcaa gttactcctg gtatctttac
     2221 agcgctaacc gcctgaaata cccgctcatt cgtaaacgac tgattgaact gtggcgcgaa
     2281 gccctcaaac aacacagcga tccggtactg gcgtgggcat cgattatgaa tgatccgcaa
     2341 aagtgcctga gctacaaaca agtgcgtggg cgcggcgggt ttatccgctc caactggcag
     2401 gaactaaacc agctgattgc cgccgctaac gtctggacca tcaaaaccta cggcccggat
     2461 cgcgttgccg gtttctcgcc gatcccggcg atgtcgatgg tttcttacgc cgccggaacg
     2521 cgttatctgt cgctgcttgg cggcacctgt taaagtttct acgactggta ttgcgacctg
     2581 ccgcccgcct cgccgatgac ctggggcgag caaaccgacg taccggaatc tgccgactgg
     2641 tataactcca gctacatcat cgcttggggg tctaacgtac cgcagacacg tacgccggac
     2701 gcccacttct ttaccgaagt acgctacaaa ggcactaaaa ccatcgccat tacccctgac
     2761 tactctgaag tggccaaatt gtgcgaccag tggctggcac cgaaacaagg cactgatagc
     2821 gccctggcga tggcaatggg gcatgtgatt ttaaaagagt ttcatctcga taatcccagc
     2881 gactacttta tcaactactg ccgccgctac agcgacatgc cgatgctggt aatgctggag
     2941 cctcgcgacg atggtagcta cgttcccggg cgcatgatcc gcgcatctga cctggtggat
     3001 ggactgggcg aaagcaacaa tccgcagtgg aaaaccgtag cagttaatac cgcaggtgaa
     3061 ttggtagtgc cgaacggttc gattggtttc cgctggggag aaaaaggcaa atggaatctg
     3121 gaatccattg ccgccggtac ggaaaccgaa ttgtcgttaa ccctgctcgg tcaacatgac
     3181 gctgttgcag gcgtggcctt cccctacttt ggcggcattg aaaatccgca ttttcgcagc
     3241 gtaaaacaca atccggtgct ggtgcgccaa ttgcccgtta aaaacctgac gttagtcgat
     3301 ggcaacacct gtccggtggt cagcgtttat gatttggtac tggcgaatta cggcctcgat
     3361 cgcgggctgg aagatgaaaa cagcgcgaaa gattacgctg aaatcaaacc gtacacccca
     3421 gcctggggtg agcaaattac cggcgtaccg cgccagtata ttgaaaccat cgcccgcgaa
     3481 tttgccgata ccgcccataa aacgcatggg cgctcaatga ttatcctcgg cgcaggtgtt
     3541 aaccactggt atcacatgga catgaactac cgcgggatga tcaatatgct gatcttctgc
     3601 ggttgtgtcg ggcaaagcgg tggcggctgg gcacactatg tcggccagga aaaactgcgc
     3661 ccacaaaccg gctggttgcc actggccttt gcgctcgact ggaaccgacc accgcgccag
     3721 atgaacagca cctcgttttt ctacaatcat tccagccaat ggcgctatga aaaagtctct
     3781 gctcaggagt tactttcacc gctcgccgat gccagtaagt acagcggtca tctgattgat
     3841 ttcaacgttc gcgccgaacg tatgggctgg ctaccttctg cgccgcagct ggggcgtaac
     3901 ccgctcggga ttaaagctga agccgacaag gccggattat cccccacaga atttaccgcc
     3961 caggcgctga aatcgggcga tttacgtatg gcctgcgaac aaccagatag cggcagcaat
     4021 catccgcgta atttgtttgt ctggcgctct aacctgcttg actcctccgg caaaggccac
     4081 gagtatatac agaagtatct gctggggacc gaaagcggga ttcagggcga ggaactcggt
     4141 gccagcgacg ggatcaaacc ggaagaagtc gagtggcaaa ctgcggcgat tgaaggcaag
     4201 ctcgacctgc tggtgacgct cgacttccgc atgtccagta cctgcctgtt ctccgatatc
     4261 gttctgccca ccgccacctg gtatgaaaaa gacgatatga acacctcgga tatgcatcca
     4321 tttattcatc cgctttctgc ggcggtcgat ccggcctggg aatcacgcag cgactgggaa
     4381 atctacaaag gtattgccaa agcattttcg caagtgtgcg tgggccatct tggcaaagaa
     4441 accgacgtgg tattacaacc actgctgcat gactctccgg cagagctctc acagccgtgt
     4501 gaagtgctcg actggcgcaa aggcgaatgc gatctgatcc cgggtaaaac cgcgccgaat
     4561 attgtggcgg tggagcgcga ctaccctgct acgtatgaac gctttacctc gctcgggcca
     4621 ttgatggaca aacttggcaa tggcggtaaa gggatttcgt ggaataccca ggatgaaatc
     4681 gatttcctcg gcaaactcaa ttacaccaag cgtgatggcc cggcacaggg gcgtccgctg
     4741 attgacaccg ccattgacgc ttcagaagtg attctggcac tggcaccgga aaccaacggt
     4801 catgttgcag tcaaagcgtg gcaggcgctg ggcgagatca ccgggcgcga acatacccat
     4861 ctggcgctgc acaaagagga cgagaagatt cgctttcgcg atattcaggc gcagccgcgt
     4921 aaaattatct ccagccccac atggtccggt ctggaaagcg atcacgtatc ctataacgcg
     4981 ggatacacca acgttcatga gttaattccg tggcgcacgc tgtcgggacg ccagcagctc
     5041 tatcaggatc atccgtggat gcgtgctttt ggtgaaagcc tggtggctta tcgcccgcct
     5101 atcgacaccc gtagcgtcag tgagatgcgc cagataccgc caaacggctt ccaggaaaaa
     5161 gcacttaact tcctgacgcc gcaccagaaa tggggcattc actcaaccta cagtgaaaac
     5221 ctgctaatgc tgacgctctc tcgcggtgga ccgattgtct ggatcagcga aacagatgcc
     5281 cgagaactga ccattgtcga taacgactgg gtggaagtat ttaacgccaa cggcgcgctg
     5341 actgcccgcg cggtggtcag ccaacgtgta ccgccgggca tgaccatgat gtatcacgcc
     5401 caggaacgca ttatgaatat tcctggttcg gaagtaactg gcatgcgcgg cggcattcat
     5461 aactcggtca cccgcgtttg cccgaaaccg acgcatatga ttggcggtta cgcgcagctg
     5521 gcctggggct ttaactacta cggcaccgtc ggttcgaacc gcgacgaatt catcatgatc
     5581 cgcaagatga agaacgttaa ctggctggat gatgaaggtc gcgatcaggt acaggaggcg
     5641 aaaaaatgaa aatacgttcg caagtcggca tggtgcttaa cctcgataaa tgtatcggct
     5701 gccatacctg ttcggtgacc tgtaacaacg tctggaccgg acgcgaaggc atggagtacg
     5761 catggtttaa caacgtcgaa accaaaccgg gcattggtta tccgaaaaac tgggaagatc
     5821 aggaagagtg gcaaggcggc tgggtccgcg atgtgaatgg caagatccgt ccgcgtctgg
     5881 gcagcaagat gggcgtaata accaaaatct tcgccaaccc ggtggtgccg cagcagattg
     5941 atgattacta cgaacctttc accttcgact acgaacattt gcatagcaca ccggaaggca
     6001 aacatatccc tactgctcgc ccgcgttcac tgattgacgg caaacggatg gacaaagtga
     6061 tctggggccc aaactgggaa gaactgctgg gcggcgagtt tgaaaaacgt gcccgcgacc
     6121 gcaacttcga ggccatgcaa aaggagatgt acgggcagtt tgaaaacacc ttcatgatgt
     6181 acctgccgcg cctgtgcgaa cactgcctca atcccagctg cgtggcgacc tgcccaagcg
     6241 gcgctatcta caaacgcgaa gaagacggca ttgtgctgat tgatcaggat aaatgccgtg
     6301 gctggcgttt gtgcattagc ggttgtccgt acaaaaaaat ctacttcaac tggaaaagcg
     6361 gcaagtcaga aaaatgtatc ttctgttatc cgcgaattga gtcaggccaa cccaccgtgt
     6421 gctcagaaac ctgcgtgggt cgcatccggt atctgggcgt gctgctttac gacgccgacc
     6481 gcatcgagga agcgtcgagc accgagcgcg aagttgacct c
//
LOCUS       DAZPCS010000185         6514 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZPCS010000185 DAZPCS010000000
VERSION     DAZPCS010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6514
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.188"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            159..506
                     /locus_tag="ACHUQ7_003561"
                     /pseudo
     CDS             159..506
                     /locus_tag="ACHUQ7_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
     gene            559..954
                     /locus_tag="ACHUQ7_003562"
     CDS             559..954
                     /locus_tag="ACHUQ7_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFR1136803.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     CIHGNAVEYILIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            995..1738
                     /gene="cmoA"
                     /locus_tag="ACHUQ7_003563"
     CDS             995..1738
                     /gene="cmoA"
                     /locus_tag="ACHUQ7_003563"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFR1136804.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            1735..2706
                     /gene="cmoB"
                     /locus_tag="ACHUQ7_003564"
     CDS             1735..2706
                     /gene="cmoB"
                     /locus_tag="ACHUQ7_003564"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFR1136805.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     TVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(2871..5300)
                     /gene="torZ"
                     /locus_tag="ACHUQ7_003565"
     CDS             complement(2871..5300)
                     /gene="torZ"
                     /locus_tag="ACHUQ7_003565"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFR1136806.1"
                     /translation="MALTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWTAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQRANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(5325..6425)
                     /locus_tag="ACHUQ7_003566"
     CDS             complement(5325..6425)
                     /locus_tag="ACHUQ7_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFR1136807.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENEPDILTRYFQYNAKDITEKQ"
BASE COUNT         1486 a         1711 c         1548 g         1769 t
ORIGIN      
        1 cataatgggc ttttaatcct atcgtttatt gcttgcatag tagaatctaa ccatccatca
       61 ctcatagcta accctcggaa atcttacatt tctgtctatc ataaccacta gcatcacctt
      121 taggaaccat ctgaaacggg aggtgtattt agaccttgct aatcttagac agccgcccgg
      181 ttcatgcatc acgtccacac agtgaagcta ttcgcgacgc tcaacgaaaa aaacctaaag
      241 ttccggtaca tgctgtactg acggcgacaa atccgctgat ccgttttatc ggtagtgatg
      301 atatgacgca aaaccgggaa ttatttcagg tctggttaca aaaattagcg cagtggcatc
      361 agaccactac gccttatctt tttttacata cgccagatat tgcccaggcc ccggaactgg
      421 tacataccct gtgggaagac ttacgtaaaa cgcttccaga gatcggagca gttccggcta
      481 ttccacagca atcttctctt ttctgaattt gccacctatc atagacaggt gccatcggcc
      541 attttaaagg gagtttgtat ggtaagcgcg ctatatgccg ttttaagtgc gttgttatta
      601 atgaagttct cttttgatgt cgttcgcctg cgaatgcagt accgcgttgc ctatggtgac
      661 ggtggtttta gcgaactgca aagcgctatt tgcattcatg gtaacgcggt ggaatatatt
      721 ctcatcgcga ttgtgctgat gctgtttatg gaaatgaatg gcgcagaaac ctggatggtg
      781 catatttgcg gcatcgtttt gcttgctggt cgtctgatgc attattacgg ttttcatcac
      841 cgtctgtttc gctggcgacg ttccggcatg agcgccacct ggtgtgcgct gttgctgatg
      901 gtgctggcga atctttggta tatgccctgg gagttggttt tctccctgcg ttagcgcaca
      961 atacgccact ttctttttcc cggattttta cgttatgtct caccgcgaca cgctattttc
     1021 tgcccctatc gccagactgg gcgactggac ctttgatgaa cgggtcgctg aagtcttccc
     1081 ggatatgatc cagcgttccg ttcccggcta ttccaatatt atttccatga ttggtatgtt
     1141 agccgagcgc ttcgttcaac ctggtacgca ggtttacgat ctgggttgtt ctctgggcgc
     1201 ggcgacgctc tcggtgcgtc gcaacattca tcatgataat tgcaaaatta ttgccatcga
     1261 caactccccg gcgatgattg aacgctgccg tcgtcatatt gacgcctata aagcccctac
     1321 gccagtagac gtcattgaag gtgatattcg cgatatcgcc attgaaaacg catcgatggt
     1381 ggtgctgaat tttaccctgc aattcctgga accttccgag cgccaggcat tactggataa
     1441 aatttatcaa ggactgaacc cgggcggcgc gctggtgctt tcggaaaaat tcagtttcga
     1501 agatgccaaa gttggtgaac tgttgttcaa catgcaccac gactttaaac gtgccaacgg
     1561 ttacagcgaa ctggagatca gccagaagcg cagcatgctg gaaaacgtga tgctgaccga
     1621 ttccgtggaa acccataaag cacgcctgca taaagccggt tttgagcata gcgagctgtg
     1681 gttccagtgc tttaactttg gttcactggt ggcattaaaa gcagaggacg ctgcatgatc
     1741 gactttggta acttttattc tctgattgcc aaaaatcatc tttcacactg gctcgaaacg
     1801 ctgcccgcgc agattgctaa ctggcagcgc gagcagcagc acgggctgtt taagcagtgg
     1861 tccaacacgg tggaatttct gcctgaaatt aaaccgtatc gtctggattt attgcatagc
     1921 gtaaccgccg aaagcgaaga gccactgagc accgggcaaa ttaaacgcat tgaaacgctt
     1981 atgcggaacc tgatgccatg gcgcaaaggg ccgttctcac tgtatggcgt caacatcgat
     2041 accgaatggc gttccgactg gaaatgggat cgtgttctgc cccatctttc tgatttaacc
     2101 ggacgcacca ttctggatgt cggctgcggc agcggttatc acatgtggcg catgattggc
     2161 gcaggcgcgc acctcgcggt ggggatcgac cctacgcaac tgtttctgtg ccagtttgaa
     2221 gcggtgcgta aactactggg taacgatcag cgcgcacatt tgttaccgtt aggtattgaa
     2281 cagcttccgg cactgaaagc ctttgatacc gtcttttcga tgggcgtgct ttaccatcgc
     2341 cgttcgccgc tggagcatct ctggcagttg aaagatcaac tggtgaatga gggtgaactg
     2401 gtgctggaaa cgctggttat tgatggcgat gagaacacgg tgctggtacc aggcgatcgt
     2461 tacgcgcaaa tgcgtaatgt ctatttcatc ccttccgcgc tggcgctgaa aaactggctg
     2521 aagaagtgtg gttttgtaga tatccgcatt gtggatgtgt gcgttaccac tactgaagag
     2581 cagcgacgca ctgaatggat ggttaccgaa tctctgtccg atttcctcga cccacatgat
     2641 ccgagtaaga cagttgaagg ttatcctgcg cctaaacgcg cggtgctgat tgcgcgcaag
     2701 ccgtaaaggt ctggtattac tgccggatgc ggcgtgaacg ccttatccgg cctacaaacc
     2761 cttgctaatt caatctattg cagggactat gtaggcctga taagcatagc gcatcaggca
     2821 gttttacgtt tgcataacct cagcgcccgt ttccgggcgc tattcacttc ttacgcctga
     2881 acagcaggct tatcaaatgc cgttaacttc ggtgcgttac ccgtatattt ttcgatatac
     2941 accagcgcag agttaccggc acaggcattt gcccgctgcg agctggggat atccgccgtt
     3001 aacacgttcg cactgccgtt tttacacaag ccgttttcca gatctggcca tgcaccttca
     3061 tgcacgcata ccacgccttt tttgatcccg tcagtcacca ccgcgcctgt caaaatctga
     3121 ccacgtttgt tccagacacg caccagatcg ccattcgcaa taccaaatcg agcagcatct
     3181 tcggtgtgaa tagtgatcgg ttcacgatcg gcaaccgcat attttttacg cagttccgca
     3241 tagttaagct gactgtgtaa acggtgtgcc ggatgtgcgg tcagaagctg caactgcttc
     3301 tcgtcggcgg tacccttcca ctcatcaggc gcaagccagg ttgggtgtgc cgggcaatcc
     3361 ttatagccaa atttttccag cgttttggag taaatctcaa ttttgccgct tggcgtaccc
     3421 agcgcatttt tcaccggatc ggcgcggaaa tcaccataac gaacgtactg ttcgttcttc
     3481 tcgctgcggc gcatttcgat cagtttattt tgctgccaga aggcattaaa cattggcata
     3541 gtgacgcgtt gcgcacgggc ccctttctga gcggcatcat agaaaaattt cagccacgcc
     3601 atttcatctt taccttcggt atagatctct tttccgccag gtttgagtaa ttccgcaaga
     3661 tcggcaaaca cgtcaaaatc gttacgcgct tcaaattgcg gagcaacagc ctgcttcatc
     3721 ggcacaatat gctggttgct gtaatcaccg gtcatcgtca agtcattgcg ctcaaacgat
     3781 gtggtgatcg gtaatacgat atctgcgtgt ttggctgctg cggtccagta gcattcagaa
     3841 acgacgatca tctccggttt ctgccaggct ttgatcagac gattagtatc ctggtgatgg
     3901 gtaaagttac cgccacccgc ccaccagatc attttgatat tcggatacgt ttgttcttta
     3961 ccgttatgct gatatttgcc gccaggattt tccagcgcat cgacgatacg ggcaacagga
     4021 atcgccgtca ttccgccatc atcagcggcc tcgctggcct gtccggcaat cgccgcagac
     4081 atttccggca gcacgccgcc aacacgcgtc gggttgccac cattggagta atgataagag
     4141 aaaccaaaac cgccgcccgg tgtaccaatc tgccctaaca tcgccgccag cgtcaccagc
     4201 atccagtgtt tctgttcacc atattgctgg cgctgaattc cccagcctgc catcagcatt
     4261 gtgcgattcg ccgccatcag ttcagcgagt ttgactatct gcgcttccgg tacgccagta
     4321 atttccgccg tccagaccgc gctcttcggc gtattatcgc tcttacctgt cagatactct
     4381 tcaaactgcg gatacccggt agtgtatttt tcaaggaaca ctttatcgtg tttgccttgt
     4441 gtcatcaggg tatgcgcaat ccctaacatc agtgccacgt cggtgcccat attcggcgcg
     4501 atccaggtgg cattatcgcc aaagaattcg atggtttcgg agcggatagg atcaatggca
     4561 atcactggtt tgccagattt tttcagctga tggaagtatt ccagcccttg ctcatcggta
     4621 ctgctccagg caatttttaa ggtattcagc gggttcattc cccacagcac cacaacctgg
     4681 ctgttttcca gaatcagcgg ccaggaggcc tgctgttcat acacctctac agaaccgacc
     4741 acatgcggca tgatcacctg tgctgccccg gttgaataat cgccgctatg cccggaataa
     4801 ccgcccgcca ggttcatata acgttgcagt aaggtttgcg ctttatgcaa cacgccagaa
     4861 gagcgccagc cgtaagatcc ggcaaaaatg gccgatggtc cgttagcttt acgaatacgc
     4921 tcatgttgct catgaatcag ttttaatgcc tgttcccagc tcacctgtac ataggtatct
     4981 tcgccacgac ctttcgccgg ttgcagtgga ttgtcgagat agctttttct caccatcgga
     5041 tgctgaatac gcgccgtggt gtgtacctga tccgccgccg tagactgtaa ggagttcggt
     5101 atggttttcg ccagcgcgcc tgtcgaagaa acaatcttgc cgtccttcac ttctacgctc
     5161 atcgctcccc aacgtcccgc ggtgaggatt ttaccgccct tctcttctgc ccatgcgggg
     5221 agcggtgctg ccgatgtcac caccagcgct ccagcggcaa taccgctgtg tttaataaat
     5281 tcacgtcttg ttaatgccat aacttcctcc ctgatcaacg aggatcactg tttctcggta
     5341 atatctttgg cgttgtactg gaaataccgc gttaaaatgt ccggttcgtt ttcgctcatg
     5401 ctggttcgtg cccccattcc tttggcaatg gacggccacg cattgacggt gtaatggtcg
     5461 gcggcaatag aggcatgaca accagcgcaa taggtatcgt caagtttttc agcgtattgc
     5521 catagcggtt tacggtccgc taatgcggga tcggtaagcg caccctgtaa agacgcctga
     5581 cgccattgat tgccgtattc gtcagcctgc cattccccgt ttaccgtgag cgccttgata
     5641 ccttcttcac ttaatgtggc tagcgccagc cgttgacctg ccgccaggta gagcgtgttt
     5701 tcactgccct gcatttgata accctgcaac agaacgatcg gctgtttgcc actggcatca
     5761 acgacggtga gatcggtacc aggattcacg gtagccagct cgcctatgcg agaagttttg
     5821 aaaggataaa tatgtgcgcc attagtcact gaagtagcgg cctgactttc cagctcatgc
     5881 gccgcgttgt catccatttt tatttctggc ggaaaatggg caatgccttt atgacaatcg
     5941 atacaggttt cgccgccctt ttgtgctttg ttatgcattt tctgcgcaga ttcactttgc
     6001 gaggcaatat ccatggcatc aaaagaatgg caactacggc acgttgcaga gtcagttgct
     6061 tttaattctt tccatactgt ttcggccatt tcctggcgat gagtttcgaa cttatcgtca
     6121 ctgtctattt tgccgctaac aaattcatga taaatatctt tagatgcttt taatttagca
     6181 aataaataat ccatccctga ctttggaata tggcaatcgg cacattccgc acgtatccct
     6241 ttctggttcg aaaagtggac agttccctga tattcctcaa aaggtttact catcgagtgg
     6301 caggaaaggc aaaatgctgt atccgacgtt ttatgtaagg ctttttgcgc cagcaataac
     6361 ccgccgccac caaccacaac cgctatcagc aaaaacaata acccaatgcg ttttttccct
     6421 cgcatagatt cctctaaata aattccagaa caccgaaatc attgtataat taaatacaca
     6481 acgaattaac cgcttgcata actcggcaca acac
//
LOCUS       DAZPCS010000186         6374 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZPCS010000186 DAZPCS010000000
VERSION     DAZPCS010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6374
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.189"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..378
                     /locus_tag="ACHUQ7_003567"
     CDS             <1..378
                     /locus_tag="ACHUQ7_003567"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1136808.1"
                     /translation="IIRWLLKKRTNGSIRYCQYTSKVAMIYIEQLELIHKSGDVLYPV
                     KITRKSSGKTAFHLVPFGLNKTHDLLEVEDASEAIRLVIDERHSIRCSTLTATITNKK
                     GKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(454..918)
                     /gene="nanQ"
                     /locus_tag="ACHUQ7_003568"
     CDS             complement(454..918)
                     /gene="nanQ"
                     /locus_tag="ACHUQ7_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFR1136809.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(915..1790)
                     /gene="nanK"
                     /locus_tag="ACHUQ7_003569"
     CDS             complement(915..1790)
                     /gene="nanK"
                     /locus_tag="ACHUQ7_003569"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFR1136810.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(1787..2476)
                     /locus_tag="ACHUQ7_003570"
     CDS             complement(1787..2476)
                     /locus_tag="ACHUQ7_003570"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFR1136811.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="ACHUQ7_003571"
     CDS             complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="ACHUQ7_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFR1136812.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="ACHUQ7_003572"
     CDS             complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="ACHUQ7_003572"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFR1136813.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(5139..5930)
                     /gene="nanR"
                     /locus_tag="ACHUQ7_003573"
     CDS             complement(5139..5930)
                     /gene="nanR"
                     /locus_tag="ACHUQ7_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFR1136814.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         1621 a         1716 c         1577 g         1460 t
ORIGIN      
        1 atcatcagat ggctgcttaa aaaaagaaca aatggtagta tccgctattg ccagtacacc
       61 tcaaaagttg ccatgattta cattgaacag cttgaattaa tccataaaag tggtgatgtg
      121 ttgtatccag taaaaataac cagaaaatca tcaggaaaaa ctgccttcca ccttgttcca
      181 ttcggcttaa ataagactca cgaccttcta gaggtggaag atgcttcaga agcaattcgg
      241 ctggtgattg atgaacgtca ttccattcgc tgcagtacac ttacagcaac tattacgaat
      301 aaaaagggca agcgaattaa gcggacaggt atatacagta tcaagggtgt taacataaaa
      361 gaatacaacg tcaggtaatg gcacagagcg gtccgtcagt tcaggtttgg ctctgtgcca
      421 aagatgggtc aggtcacacc tgatctaata aatttaagcc attaaatcag ccttaacctt
      481 caccaccacc tttttaatct cacccggctc gccaacaacg catcccggct tatgcggctc
      541 tcctggcata aacacggcga acattcccgg tttcagaatg atggtttgct cgttttcaat
      601 ggcgctacaa agctggtaat catcttcatg gtggaactct tcacattggc gcgcagtgcc
      661 tgccatgcca aacagaatcc gttcctcacc gtttaataac agctggatat caatgtattg
      721 ctcgtgcaat tccgcttttt tctcgacggg cgattgagtg ttaaacgtca tgacattcat
      781 aaaaatattg tcgccctgta attcgtaacg acccggcgct ttttcttgcg gtctggcagc
      841 taaggccagc gttaacgcgt cctgtaacgc aggatgtaac ccagcagacg gtaatgactg
      901 tacttcaccc atcatcataa tttttctccc tgggccaaca gcgcagcccc aagtaaacct
      961 gcatcatggc ggtaatgcgc cgccagtaaa tcaacatgaa atgccgctgg ctcctgcgcc
     1021 agatacattt ccaccaacgc cagataccct tctgccagac caacgctgcc accaaccacc
     1081 acgcactggc aatcagttgt ggctttaata tcagcgatca gccttgcaag cgtacgtgcg
     1141 gagcgatgaa tcagttgctg cgcctgctca tcgccctgcc cggcgcgtgt gaaaatagtt
     1201 ctcgcatccg cgccagccag ctcgccctgc gctgccgccg caatgccgcg accagaagca
     1261 attgcttcca cgcaacctgt gcgtccacag ccgcagactg ggccgtgtgg atcggcaagc
     1321 gtatgcccga tgtgccccgc cagaccgcca gggccggtaa gcagtttgcc gccgctcact
     1381 acaccgccgc cgacgccagt ggaaacggtg ataaagacca tatcggttat atcgccttcc
     1441 agcgcctgat actctgccca tgctgcggcc tgcgcgtcgt taatggcaat ggtcggcaaa
     1501 ttggtaagtt gttccagcgt tttgactaac ggaaagtgta gcaatccacc gagattatgc
     1561 ggattcagcg ccagcaaact gccgtcgcga ataattccgg ttgaagcgat ggcaacccgc
     1621 tgcgcatgag cttgcaacgg agagactaat gcggataagg catcacgcaa ggcttctggt
     1681 gtctggctgg ctggcgtagg aagttcacga cgatcgcgga tctgcccgtc agcgccaatc
     1741 agcgcggcgg caagtttagt accgccgata tcaatcgcca gtatggtcat agcaccgcct
     1801 ttttcatcgc tgtgttgtac cactgacaaa tgtgttcaag acgcgtaatg gcagaaccga
     1861 ccgtcaccgc ccacgcgccg tggcgcatcg catctgccgc ctgagcaggc gtgttgtaac
     1921 gcccttcggc aatcacccga catccggctt cgctcaacgt tttcaccagc gccagatccg
     1981 gctcttctgg cgtttcaggc gtggtatagc cagaaagcgt agtgccaata atttcggctc
     2041 ccagcttttg gcatgccagg ccgtcttccg gcgttgagca gtcggtcatc gccagtaaac
     2101 cgtgatgatg gatacgtgcc agcagcgttt caacaggcac cggacgcggg cggtcggtgc
     2161 cgtcaatggc gataatgtcc gcgcccgcct gcgccagcgc atcgacatct tcaatatagg
     2221 ccgtgatgcg taccggagaa tcctccagat cgcgtttcac aattccaata atcggcacgc
     2281 tcaccaccgc acgcgtggct tgcagatttg ccacaccttc gatgcgaatg gcaaccgcgc
     2341 ccgcctgctc tgccgctaat gccatggctg cgacgatttc gggtttatcg agcgggctgt
     2401 caggaaccgg ctggcaggag acaatcaggc caccgttagc agcgattttt tgatccagtt
     2461 gtgcaagtaa cgacatacat cttcccttag cgaaaggccc ggtacataga ccgggcaaca
     2521 ggattaactt ttggttttga ctaaatcgtt tttggcgctg ccaaatggca cggcaccgct
     2581 aaatggtttg ccgtcgatag cgtcatgagt acgcaatgct tccgggcgca gccaacgctg
     2641 aacgcgggaa ggcatatcca gaccaatcag caggatcacc acaaatgtca ggctgaacga
     2701 gagcgatgcc agcgcagtac ccagatccag acgttgagcg atcaacgcgc cgatgattgg
     2761 ggccagtgcg ccacccaatg cgccaacgtt gtaggtaaag cccaggcccg ctgcacgctg
     2821 gtcggtatcg aaataaccgc caatcagttt cggtaagatc ccggcgatcc cttgtccaag
     2881 catttgctgg aagaacagta acagaccgag cacccagacg tttgcgccgc caatcgcaaa
     2941 taccggaata atcagcagct gcgaggccag caggctacaa acgtacgctt tgcgggttcc
     3001 cagccagtca ccgaggaagc cacctacgca gcatcccacg gccgcgccaa agccactaaa
     3061 gaacagcaca ttggcgacag tatgcgggtt ataagccaga tcggttttca gatacgttgg
     3121 cagcagcgcc tgaatcggcc atgagtagag gaaagcaaac aacacgacca ccatcagcat
     3181 tacgcccgtt ggccagcgtt tacccgtact ttgcaccata aagctgataa agattgcggc
     3241 gcataacagc ccgagaacag cgacgattgc agcattttgc aggttaccgg cgaagcagaa
     3301 ccacagcgca gtagccgccg ccagtgtcat tacgatattg gcaatgcgat gttcaccacg
     3361 gtagagaata tccaccattg tgcgtactgg tgctttacct gcgtgtttct ctttccagtc
     3421 ttccgcttcc gggatgtttt tacgcagcca gagagcaaag atgattggca aaatgccgat
     3481 aaagaacagc gcacgccaac cccagaccgg aaccaccagg ctatagacct gagcggcaac
     3541 gacggccccc acagagaagc ctgaaatcaa aaaaccactg gctttgttac gcagatgttt
     3601 tggccagctt tcaatgacat aggtggcgct ggaaccgtat tcacccgcca tccccatgcc
     3661 gatgaccaga cgagcgataa acatggtgat gtagcctggc gcaaagccgc aggccagcgt
     3721 cccggccgag aagagaacga tgctggtgac cattgccaga cgacgcccgt agcggtcacc
     3781 catagcgccg agcatcaggc cgccgaacca gcgagagata aaggctgcgg agatcagact
     3841 tgccgcctgc accgtcgtca gcccgaattc accctgtact tcggtgagta ccagggcgat
     3901 taaaacgaaa tcaaaaccgt caagcagata tcccaaccag gcagcggaaa atgcgcgcca
     3961 ttgtgcacgg ttgagatggc gataccacgg gatattctgg gttgtagtac tcattgtgag
     4021 tctcccgcgg tgggcgatgc ccacacgctt tggtatgaaa attgtagggt acagatgcgt
     4081 ttatttcccc tcacccggta ggggcgagcg aggggaaaac aactcacccg cgctcttgca
     4141 tcaactgctg ggccagcgcc ttcagttctg gcagatattt ttcatctacc ggtccaaacg
     4201 gtttgcggca cagcggcaca gaaacgacat ccatataatg caggacagtt ttcaggccgc
     4261 ggaatacgcc cgttttgatc agtaaatcaa tgactttgtt gcactcagtt tgcagtttct
     4321 gcgcggtctg gatatcgcct tctttcagcg ccttcacgat cccctgatat cgccagccca
     4381 tgatgttgta agtactgccg ataccaccat cagcgcctgc cagcaagccg gaagcgaaga
     4441 tttcgtcgta accgttatag agcaccagat caggatgttc acgacggatc tgttccatct
     4501 gatagagatc gccagaagtc tgtttcagcg caccgacgcc aggcaacgta accagggtgt
     4561 tgatctgatc cagggtcagt tttaccccac tcagggctgg aatgttgtac accaccatcg
     4621 gcaaaccatc cgctgaatca ataattgccc gatagtgatc gcagtgttct tcaaagctga
     4681 aaggatagta gaacggcgtg acggcggaga cggcatcgaa gccataacgt ttagccgatg
     4741 ccgcaagttg ttggctttcg gcggtgctga cgcaaccgac gtgggcgatg agtttaatct
     4801 tacctttcgc ctcttcggcg acgatttcca gtacctgttc acgctcggaa aggctttgta
     4861 caaaggcttc gccggtcgaa ccacccacgt ataaaccgtc gatgccctgc tgaatattga
     4921 actgaaccag gcggcgcaga ctcgctttat ccagtgcttg ttgttgatca aaaggagtca
     4981 ggagtgcagc cattacgcca cgtaaattcg ttgccataaa tacctctgaa gtgatgcttg
     5041 tctgataaac gatatacctt tatacctgtt ataccagatc aattaagcaa caccccatac
     5101 agaaagctta taatgcgatc tgcttcacta aagtggcatt atttcttttt gttggtggtc
     5161 tgaccgaaag cgtgccaggt agcagagacg ctgttgagat gcgattgcaa cgcacgatcg
     5221 gcttcgtcag gatcatgacg gcggatcgca tcaacgatcg caatatgctg ttgataacta
     5281 acgttgttat gttcgtgcag tgcctgatcg gtaaccgttg ggcgtgcggc aataagccag
     5341 tcgagcaggg caacgtggat cgccatgaag attgggttac cggggatctc cgccagcacg
     5401 cggtggaaat caacgtctga acgaatgaat gccgcgttgt tatccagcga ctgactgttg
     5461 atttccagtg cttttgccag caaatcgatt tgctcatcgg tggcgtgttc agccgcatag
     5521 cgcaccaggc tggattcaaa gaacagacgt aattgttcga aatgggcaat cccaccagga
     5581 tgagaaagga aatctttcgc catgccggaa agctcgccga tgatagtgtc cgcagaaggt
     5641 cgagagacac gagcgcgttc gccgttgttt atttgcacca gacctttgcg ttttaacgct
     5701 gccagcgctt cacgcacaga aggacgcccg acgttaaaga acgccatcag ttcgcgttca
     5761 gacggtaatt gttcaccttc gccaaattca cgacggcgga tcatctgttc cagctcttct
     5821 tccaccattt cggagagttt tttacgcgcc agcgggcggc tacgcaagtt gcgaccaatt
     5881 gcaggtgaag aatcttcggt ttgcgaatca aatgcgttca taaggcccat tctgtaaggt
     5941 cagtgtgatt aacatcatca gtgacatcct atcacaggat tgaaagtagg ggaaaatggc
     6001 aggtttttct ctttgtgcct catcattacc ataattaacg gaataattaa ctattgcgaa
     6061 aaattaatgt aacgcagata aacacatccc gattgaatta ttcataagac tattcacaag
     6121 cattatgaat attatgaatg tgttcttaca aaataatcat aatcgcatat tttttaatga
     6181 aaaatcacct cacctacaat taaaaacacg acatccgcac cataaataga cttgcaaaaa
     6241 atataacatc attgttttca atctgccatt tatgggattg accgttttct tttgacacgg
     6301 agttcaacaa tgggtaatgc ttccaactta ctgatttagt gtatgatggt gattttaagg
     6361 tgcttgcgtg gctt
//
LOCUS       DAZPCS010000187         6124 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZPCS010000187 DAZPCS010000000
VERSION     DAZPCS010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6124
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.190"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..84
                     /locus_tag="ACHUQ7_003574"
     CDS             <1..84
                     /locus_tag="ACHUQ7_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1136815.1"
                     /translation="YESVCIAAMEHLKSKATAPIQNGPLPA"
     gene            complement(88..1478)
                     /gene="tcyP"
                     /locus_tag="ACHUQ7_003575"
                     /pseudo
     CDS             complement(88..1478)
                     /gene="tcyP"
                     /locus_tag="ACHUQ7_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
     gene            complement(1611..2201)
                     /locus_tag="ACHUQ7_003576"
     CDS             complement(1611..2201)
                     /locus_tag="ACHUQ7_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR1136816.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(2364..3032)
                     /gene="hxpB"
                     /locus_tag="ACHUQ7_003577"
     CDS             complement(2364..3032)
                     /gene="hxpB"
                     /locus_tag="ACHUQ7_003577"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFR1136817.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3179..3715
                     /locus_tag="ACHUQ7_003578"
     CDS             3179..3715
                     /locus_tag="ACHUQ7_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFR1136818.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(3756..4616)
                     /locus_tag="ACHUQ7_003579"
     CDS             complement(3756..4616)
                     /locus_tag="ACHUQ7_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFR1136819.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACDWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4722..5012)
                     /gene="ghoS"
                     /locus_tag="ACHUQ7_003580"
     CDS             complement(4722..5012)
                     /gene="ghoS"
                     /locus_tag="ACHUQ7_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFR1136820.1"
                     /translation="MASGDLVHYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(5113..6042)
                     /gene="pfkB"
                     /locus_tag="ACHUQ7_003581"
     CDS             complement(5113..6042)
                     /gene="pfkB"
                     /locus_tag="ACHUQ7_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFR1136821.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
BASE COUNT         1573 a         1545 c         1515 g         1491 t
ORIGIN      
        1 tatgagtcag tatgcatcgc cgcgatggaa catttaaaaa gtaaagcaac ggcccctata
       61 caaaacggac cgttgccagc ataagaatta acggtgtgcc agttcggcgt cgtcttcgct
      121 atccagaatg gctttatcgg tttgcttcag ccactggctg gtcagcgtgc cagctgtcat
      181 cgagccacta acgtttagcg ccgtacggcc catgtcgata agcggttcaa cggagattaa
      241 cagcgccacc agggttactg gcaggcccat cgcaggcagc acaatcagtg cggcgaaagt
      301 tgcaccacca ccgacaccgg caacgcctgc ggaactaacg gtaacaatac cgaccagcgt
      361 cgcaatccac atcgggtcca gcgggttaat gccaaccgta ggcgcaacca tcaccgcagc
      421 attgccggat acaaaccggc gcagccgttc tgaccaatgg ttgcaccgaa agaggcggcg
      481 aaactggcga tggattcagg aacgcccaga cgacgcgttt gtgcttccac attcagtggg
      541 atagacgcag cactggaacg gctggtaaag gcaaacgtca gcacaggcca taccttacgg
      601 aagtacttca gcggactcac gccattaatg cccagcagaa tgccatgcac agcaaacata
      661 atcagcagac cgaggtagga cgcgacaacg aaacttccca gtttgatgat gtcttgcaag
      721 ttagaacctg caaccacttt ggtcattaga gccagaacgc cgtaaggggt caactgcatg
      781 acaaggcgaa ccagtttcat cacccagctt tgtagggtat cgatagcggt taagacgcgt
      841 tcccctttcg gcgcatcatc cttcagcagt ttcaatgcag ctacgccgag gaatgcggca
      901 aaaattacca cgctgataat tgacgtcgga ttggctccgg taagatcggc aaacgggttt
      961 ttcgggataa aggacaagac cagctgcgga acgctctgat cagagacttt accaacatag
     1021 ttactttcaa tggcgttcag acgtgcagtt tctgcaccac cctgaaccag accttcagcc
     1081 gtcaaaccaa acaggttggt gaccagcaca ccgaccagcg ccgcaatcag cgtggtaaac
     1141 aaaagcgtac cgatggtcag aaaactgatt ttgcctaact gagatgcgtt atgcagacgg
     1201 gcaaccgcgc tcagaataga ggcgaagact aacggcataa cgatcatttg cagcagttga
     1261 acatagccgt taccaacgat gttaaaccac tgtacagaat ctttaagtac ctggctgtca
     1321 gaaccataaa tggtatgcag ggcaaggcca aaaaccacac ccatcaccag acccaccagc
     1381 acttttttcg ccagactcca ctgtttatgg cgggtctgag ccagcgcaaa cagcagtaca
     1441 acgaacacca cgatgttcgc aattaatgga aagttcatcc ccgttctcct gatcttatta
     1501 tttctatcac tgcgtagcag tcatagtgtt ggcgcaagat taacagaact gtgagctggc
     1561 tcttatattc aaatggaatt gcttattcca gaaaggtgat ttttgccttt ttagtattca
     1621 acctgatgct taataagccg atgaaactgg atctgcaagg tattgatcat ttgcgatgac
     1681 caacgaacag cgctgttctc gggtaatgaa tggggcatcc ataccgacca gacaaataat
     1741 gccatgcaga taaaacgttc cagttggttg cccttttggg gaaccaggat aggcaagcgg
     1801 aaacgccagc ggcatggcca gagcagggga acaccggcgg gtgtcagcat atcggcaagt
     1861 atgtggctca aataacccag caccattcct tgtagcgcat cagccggaat gaaccagcct
     1921 tccggaacct taaggtaaaa ggttgccagc agcgcaaata ccgccagcag actgtgggta
     1981 aaaccacgat gcccaaaagc gcgggcgatc ggttttgata tccattttaa tcgctgccca
     2041 agaaacgact ttgggtgatc gatgtccggt aacaaacacg tcaggattgc ggaagggaca
     2101 atatgccacc agtcaccctg tgccagcacg ggcgtcagct cggcattttt ggcaaatacc
     2161 gcacaagcaa tagaaaagag aaggtgacct tccgccgtca tgataaaact cactaaactg
     2221 tcgatacgta cagtatagga tttttataca gtgcacggaa gtggtgacgg tgtaactctt
     2281 tctcttaaat tgacgattaa tccgtcagtt gaatgcaaat tcagataaag aaccaggggc
     2341 agcgtgatca ctgcccctgg tcattaaccg agaaggtctt ttgcggtgag ttctgtcagc
     2401 gatgagagtt tgacgtttgc taatacaaaa cgtggatcat tttgcgcttc tggcgcagga
     2461 acgacgatgg aacgcatgcg ggctgcttta gaagcgatca tgccatttac cgagtcttcc
     2521 agcgccacgc aggtcagtgg gtcaacgccc agttttgctg cgcagtcgag atatacttgc
     2581 ggatgcggct tgctgtaagg cagtttttca gccgaggcga gggcatcgaa actgtcgcgt
     2641 aagtcaaaca tggtcaacac tttttccagc atatgtagtg gtgacgcgga ggccagtccc
     2701 accaataaac cttgttcttt gcataacgca acagcttcgc gcacgcctgg taataatgga
     2761 cgtgtctctt caaccagtga aatggcacgg gcaataaccc gttctactac ttcctgacgg
     2821 cttggcccat tccacggttg ccgggcgtac caaagatcga ccaccatatc gatgcgtaaa
     2881 cctaaggtgt ccggcagctc gttacgacgg gagatatcta cccccaggct tgccatcaca
     2941 tccagttcgg ctcgatccca taaaggttct gagtcgataa gtaatccatc catatcaaaa
     3001 attgcagcaa gaatctgacg cggggttgac attacaacct ctcctttaga gcattatgtg
     3061 taaggtatta agccgtgctt atgattaagc gcctatcata tcgcctttaa agaatgggcg
     3121 aaaacagata tctacaggta aactaagtga aacgacgcgt cttaatcaag gagaactcat
     3181 gacgtatcaa caagctggac gcattgctgt tttgaaaagg attttgggtt gggtgatttt
     3241 tatacctgca ttgatctcta cattgatttc tttgctgaaa tttatgaata cccggcagga
     3301 aaaccaggaa ggcattaatg cggtcatgct cgactttgct catgtcatga tcgatatgat
     3361 gcaggcgaat acgccttttt tgaatctgtt ctggtataac tccccgaccc ctaattttaa
     3421 tggtggtgtg aacgttatgt tctgggtgat tttcatcctg atttttgtcg gactggcgtt
     3481 gcaggattcc ggtgcccgga tgagtcgcca ggcgcgcttc ttgcgggaag gtgttgaaga
     3541 tcaactcatt ctggaaaaag ccaaagggga agaggggcta acgcgtgaac aaattgagtc
     3601 ccgtattgtt gttccgcacc atactatttt cctgcagttt ttctcactat atatcctgcc
     3661 ggttatttgt attgctgcag gttatgtgtt cttttctctg cttgggttta tttaatcaaa
     3721 aggccgcacg gagcggccat tatcctcaac ccatatcatg ctgctaataa tctatccaat
     3781 gactgctgag caataaccaa atgctgaccg ccaaataatc ttgcacgatt taacagcgtg
     3841 tagagttggt aaaccggttg acgttcaagg aaatcggcag gtagcgggga tactgactga
     3901 tagccgtcat agatttgtgg cggctgttca gtatgcagcg gtaacatcgc taggtcgcac
     3961 tctcggtcac cccagtcgca ggcagggtcg aaaatgtacg ggccatccgg acccagcgca
     4021 cagttgccgg accataaatc gccgtgcaac agagaaggct gcggttgatg cgaggccaga
     4081 cgctgctgaa tatgctcgac gatggcgtcg atattgccga aagcgatccc tttctctgct
     4141 gccagttcca actgccagcc aatccgttgt tcagcaaaaa acgttgacca gcgacgttgc
     4201 caggtgttgg gctgtggagt tgtggagagc gagttatcaa aatcgaggcc aaattgtggt
     4261 tggtcactcc attgatgtaa acgcgcaatt tgctgaccaa gaataaatgc gctatgcgca
     4321 tccagcggac gaggtgggag ataatccatc accagaaaac tgtagtcacg gtcagcgcct
     4381 actgcccaaa ccttaggcac ggtgacggtt ttactacgcg acagtaactc cagttggtcg
     4441 gcttcggcgg taaaaccggg aagcagttcc ctttcatcac atttgacgaa aaagtcatgt
     4501 cctgcatagc gcaaatgcca tgcggcatgg acttctccgc caggcagttc attacgcagt
     4561 tcgatttcgc cttcacctaa ctgctcgctc aaaagacgac tgattgcctg ccacatgatg
     4621 tctctcccat gttgtctgcc agatcataag gttagcgcaa gaatgcggtg taaaaacacg
     4681 agctaacgca caccacagac tgattattgc ccaaaaacca tttattttct gttgctattc
     4741 cattcctccc aggtcgtgat ggtgatttca ggatctttgc cggttgcact ttgggtaagc
     4801 cccgaaacca gttctctaat ttcttcttca ctttgggtac tgataagtcc aaaagtgtta
     4861 gtccccagct catggatatt tccctcatca tccgtcatgg tgagcaaaaa accgtcgcga
     4921 gtcaggtaat tattcaactc gttaatttca gtcaatgtat cctcatgcaa catgacggtt
     4981 atgacataat ggacaagatc gccactggcc atagttcacc cctttgttat cacatgactt
     5041 ttgagcatag tcggagaaac gcgttgccga caggttggtg gtgattcccc caatgctggg
     5101 ggaatgtttt tgttagcggg aaaggtaagc gtaaattttt tgcgtatcgt catgggagca
     5161 cagacgtgtt ccctgattga gtgtggctgc actccccgca gctacgccaa aacggaccat
     5221 ctcttcaaga gaggcatttt ctgccagttt cagtgtcatc gcgccgacca tgctgtcacc
     5281 agcgccaacg gtactctggc ttttcaccgg tggtggcacc acctgaatgc agttttcact
     5341 atcgacaccc agcgctcctt gtggaccaag ggaaacgaca acccgtttgg ccttgccgct
     5401 attaacgatt tcctgcgcgg ctttgcggac atcgtctggc tgggtgagtt cgcgattcac
     5461 cagcgcactg agttcttttt ggttaggctt aaccaactcg atgttaccaa tcgccagtgc
     5521 tgcacttaac gcttcgccag aactgtcgat gatgcagcgg atcccttggt tttgcgcagc
     5581 ggaaatcagt tgggttaatt tttccagctt cacacctggc ggcaggcttc cgcttatgac
     5641 caggatggcc ccggattcaa tttccagaac ttgctcttca agctggcgaa actcatcttc
     5701 atttaatgcc gcgcctggca taacaaaacg atactgttca ccgcttgctt ccacatgtac
     5761 gtgtaaattc tgccgggtcc agtctttggc ttctacagta gcgacgggga cattttcatc
     5821 cgccaacagt gaaaccaggt gttcgccggt cgcgccaccc gccgggaaga tcgctgtggc
     5881 actgcctcca agatgggcaa tggcgcgggc gacgttgatg ccgccgcccc cgggttcgaa
     5941 caccggtgcg gtacagcgca gttttccttc gggataaatt tgcggggtaa ttgttgcgct
     6001 atcgagagag ggcgcaagtg tcaacgtata gatacgtacc atcatttcct cctataggct
     6061 gatttcagtc tggcaccgaa tcagcggaaa gtgaagaaat taacaatatg atttattaga
     6121 gctt
//
LOCUS       DAZPCS010000188         6105 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZPCS010000188 DAZPCS010000000
VERSION     DAZPCS010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6105
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.191"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..315
                     /gene="ipgC"
                     /locus_tag="ACHUQ7_003582"
     CDS             <1..315
                     /gene="ipgC"
                     /locus_tag="ACHUQ7_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone IpgC"
                     /protein_id="HFR1136822.1"
                     /translation="IEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAV
                     AFALGKNDYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDI
                     KE"
     gene            321..2063
                     /gene="ipaB"
                     /locus_tag="ACHUQ7_003583"
     CDS             321..2063
                     /gene="ipaB"
                     /locus_tag="ACHUQ7_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HFR1136823.1"
                     /translation="MHNVSTTTTGFPLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LTANQNINTTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTRLSELDPESPEKKKLSREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGS
                     VASAVFQNSASTNLADLTLSKYQVEQLSKYISEAIEKFGQLQEVIADLLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            2026..3174
                     /gene="ipaC"
                     /locus_tag="ACHUQ7_003584"
     CDS             2026..3174
                     /gene="ipaC"
                     /locus_tag="ACHUQ7_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HFR1136824.1"
                     /translation="MLQKQFCNKLLLDTNKENVMEIQNTKPTQILYTDISTKQTQSSS
                     ETQKSQNYQQIAAHIPLNVGKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKD
                     LSEMSHTLTPENTLDISSLSSNAVSLIISVAVLLSALRTAETKLGSQLSLIAFDATKS
                     AAENIVRQGLAALSSSITGAVTQVGITGIGAKKTHSGISDQKGALRKNLATAQSLEKE
                     LAGSKLGLNKQIDTNITSPQTNSSTKFLGKNKLAPDNISLSTEHKTSLSSPDISLQDK
                     IDTQRRTYELNTLSAQQKQNIGRATMETSAVAGNISTSGGRYASALEEEEQLISQASS
                     KQAEEASQVSKEASQATNQLIQKLLNIIDSINQSKNSTASQIAGNIRA"
     gene            3225..4223
                     /gene="ipaD"
                     /locus_tag="ACHUQ7_003585"
     CDS             3225..4223
                     /gene="ipaD"
                     /locus_tag="ACHUQ7_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HFR1136825.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNQALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSRH
                     EYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINDINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKKALEELKEKYKDKPLYPANNTV
                     SQEQANKWLTELGGTIGKVSQKNGGYVVSINMTPIDNMLKSLDNLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            4232..>6105
                     /gene="ipaA"
                     /locus_tag="ACHUQ7_003586"
     CDS             4232..>6105
                     /gene="ipaA"
                     /locus_tag="ACHUQ7_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HFR1136826.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNGLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLVDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKAFNHTPDN
                     SDGIGSDLLTTSSQERSANNSLSRGHRPLNIQNSSTTPPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANIRNYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSKIDDTSAELLTDDISDLKNNNDITAENNNIYKAAKDVTTSLS
                     "
BASE COUNT         2275 a         1241 c         1012 g         1577 t
ORIGIN      
        1 atagaggaag ctgaagtttt cttcaggttt ttatgtatat acgactttta caatgtagac
       61 tacattatgg gactcgcagc tatttatcag ataaaagaac agttccaaca agcagcagac
      121 ctttatgctg tcgcttttgc attaggaaaa aatgactata caccagtatt ccatactgga
      181 caatgccagc ttcggttgaa agccccctta aaagctaaag agtgcttcga actcgtaatt
      241 caacacagca atgatgaaaa attaaaaata aaagcacaat catacttgga cgcaattcag
      301 gatatcaagg agtaattatt atgcataatg taagcaccac aaccactggt tttcctcttg
      361 ccaaaatatt ggcttccact gagcttggag acaatactat ccaagctgca aatgatgcag
      421 ctaacaaatt attttctctt acaattgctg atcttactgc taaccaaaat attaatacaa
      481 ctaatgcaca ctcaacttca aatatattaa tccctgaact taaagcacca aagtcattaa
      541 atgcaagttc ccaactaacg cttttaattg gaaaccttat tcaaatactc ggtgaaaaat
      601 ctttaactgc attaacaaat aaaattactg cttggaagtc ccagcaacag gcaagacagc
      661 aaaaaaacct agaattctcc gataaaatta acactcttct atctgaaact gaaggactaa
      721 ccagagacta tgaaaaacaa attaataaac taaaaaacgc agattctaaa ataaaagacc
      781 tagaaaataa aattaaccaa attcaaacaa gattatccga actcgaccca gagtcaccag
      841 aaaagaaaaa attaagccgg gaagaaatac aactcactat caaaaaagac gcagcagtta
      901 aagacaggac attgattgag cagaaaaccc tgtcaattca tagcaaactt acagataaat
      961 caatgcaact cgaaaaagaa atagactctt tttctgcatt ttcaaacaca gcatctgctg
     1021 aacagctatc aacccagcag aaatcattaa ccggacttgc cagtgttact caattgatgg
     1081 caacctttat tcaactagtt ggaaaaaata atgaagaatc tttaaaaaat gatctggctc
     1141 tattccagtc tctccaagaa tcaagaaaaa ctgaaatgga gagaaaatct gatgagtatg
     1201 ctgctgaagt acgtaaagca gaagaactca acagagtaat gggttgtgtt gggaaaatac
     1261 ttggggcact tttaactatc gttagtgttg ttgcagcagc tttttctgga ggagcctctc
     1321 tagcactggc agctgttggt ttagctctta tggttacgga tgctatagta caagcagcga
     1381 ccggcaattc cttcatggaa caagccctga atccgatcat gaaagcagtc attgaaccct
     1441 taatcaaact cctttcagat gcatttacaa aaatgctcga aggcttgggc gtcgactcga
     1501 aaaaagccaa aatgattggc tctattctgg gggcaatcgc aggcgctctt gtcctagttg
     1561 cagcagtcgt tctcgtagcc actgttggta aacaggcagc agcaaaactt gcagaaaata
     1621 ttggcaaaat aataggtaaa accctcacag accttatacc aaagtttctc aagaattttt
     1681 cttctcaact ggacgattta atcactaatg ctgttgccag attaaataaa tttcttggtg
     1741 cagcgggtga tgaagtaata tccaaacaaa ttatttccac ccatttaaac caagcagttt
     1801 tattaggaga aagtgttaac tctgccacac aagcgggagg aagtgtcgct tctgctgttt
     1861 tccagaacag cgcgtcgaca aatctagcag acctgacatt atcgaaatat caagttgaac
     1921 aactgtcaaa atatatcagt gaagcaatag aaaaattcgg ccaattgcag gaagtaattg
     1981 cagatctatt agcctcaatg tccaactctc aggctaatag aactgatgtt gcaaaagcaa
     2041 ttttgcaaca aactactgct tgatacaaat aaggagaatg ttatggaaat tcaaaacaca
     2101 aaaccaaccc agattttata tacagatata tccacaaaac aaactcaaag ttcttccgaa
     2161 acacaaaaat cacaaaatta tcagcagatt gcagcgcata ttccacttaa tgtcggtaaa
     2221 aatcccgtat taacaaccac attaaatgat gatcaacttt taaagttatc agagcaggtt
     2281 cagcatgatt cagaaatcat tgctcgcctt actgacaaaa agatgaaaga tctttcagag
     2341 atgagtcaca cccttactcc agagaacact ctggatattt ccagtctttc ttctaatgct
     2401 gtttctttaa ttattagtgt agccgttcta ctttctgctc tccgcactgc agaaactaaa
     2461 ttgggctctc aattgtcatt gattgcgttc gatgctacaa aatcagctgc agagaacatt
     2521 gttcggcaag gcctggcagc cctatcatca agcattactg gagcagtcac acaagtaggt
     2581 ataacgggta tcggtgccaa aaaaacgcat tcagggatta gcgaccaaaa aggagcctta
     2641 agaaagaacc ttgccactgc tcaatctctt gaaaaagagc ttgcaggttc taaattaggg
     2701 ttaaataaac aaatagatac aaatatcacc tcaccacaaa ctaactctag cacaaaattt
     2761 ttaggtaaaa ataaactggc gccagataat atatccctgt caactgaaca taaaacttct
     2821 cttagttctc ccgatatttc tttgcaggat aaaattgaca cccagagaag aacttacgag
     2881 ctcaataccc tttctgcgca gcaaaaacaa aacattggcc gtgcaacaat ggaaacatca
     2941 gccgttgctg gtaatatatc cacatcagga gggcgttatg catctgctct tgaagaagaa
     3001 gaacaactaa tcagtcaggc cagcagtaaa caagcagagg aagcatccca agtatctaaa
     3061 gaagcatccc aagcgacaaa tcaattaata caaaaattat tgaatataat tgacagcatc
     3121 aaccaatcaa agaattcgac agccagtcag attgctggta acattcgagc ttaatatatc
     3181 caagagccat aataatatat ggctcttcct gtaaggaaat aaccatgaat ataacaactc
     3241 tgactaatag tatttccacc tcatcattca gtccaaacaa taccaacggt tcatcaaccg
     3301 aaacagttaa ttctgatata aaaacaacga ccagttctca tcctgtaagt tcccttacta
     3361 tgctcaacga cacccttcat aatatcagaa caacaaatca ggcattaaag aaagagcttt
     3421 cacaaaaaac gttgactaaa acatcgctag aagaaatagc attacattca tctcagatta
     3481 gcatggatgt aaataaatcc gctcaactat tagatattct ttccaggcac gaatatccaa
     3541 ttaataaaga cgcaagagaa ttattacatt cagctccgaa agaagccgag cttgatggag
     3601 atcaaatgat atctcataga gaactgtggg ctaaaattgc aaactccatc aatgatatta
     3661 atgaacagta tctgaaagta tatgaacatg ccgttagttc atatactcaa atgtatcaag
     3721 attttagcgc tgttctttcc agtcttgccg gctggatctc tcccggaggt aacgacggaa
     3781 actccgtgaa attacaagtc aactcgctta aaaaggcatt ggaagaactc aaggaaaaat
     3841 ataaagataa accgctatat ccagcaaata atactgttag tcaggaacaa gcaaataaat
     3901 ggcttacaga attaggtgga acaatcggca aggtatctca aaaaaacggg ggatatgttg
     3961 tcagtataaa catgacccca atagacaata tgttaaaaag cttagataat ctaggtggaa
     4021 atggcgaggt tgtgctagat aatgcaaaat atcaggcatg gaatgccgga ttctctgccg
     4081 aagatgaaac aatgaaaaat aatcttcaaa ctttagttca aaaatacagt aatgccaata
     4141 gtatttttga taatttagta aaggttttga gtagtacaat aagctcatgt acagatacag
     4201 ataaactttt tctccatttc tgaggtgcgc catgcataat gtaaataata ctcaagcgcc
     4261 aacattctta tataaggcaa cttcaccatc atcaacagaa tacagcgagt taaaaagcaa
     4321 aatatccgat atccatagtt cgcaaacttc tctaaaaaca ccagcatcag tgtctgaaaa
     4381 agaaaacttt gcaacgtctt ttaatcagaa atgtcttgat tttttatttt cttcctcagg
     4441 gaaagaagat gtgttaagaa gcatttattc caactcaatg aatgcgtatg ccaaaagcga
     4501 gattctcgaa ttttcaaatg ttttgtactc cttagtacat caaaatggtc ttaattttga
     4561 aaacgaaaag ggacttcaaa aaattgtcgc acagtattcg gaactaatta taaaagataa
     4621 attatcccaa gattctgcct ttggaccatg gtcggcaaag aataagaaac tccatcaatt
     4681 acgacaaaac attgagcaca gacttgcact attagcacaa caacacacat ctggtgaagc
     4741 tttatcattg ggacaaaaac tcctcaatac tgaagtatca tcatttatca agaataatat
     4801 tcttgctgaa ttaaagttaa gtaatgaaac tgtttcatct ctcaaactag atgatttagt
     4861 tgacgcacag gcaaaacttg cctttgatag tttgcgcaat caacgtaaaa atactattga
     4921 tagtaaagga tttggtatag gtaaactgtc aagagactta aatacagtag ccgtgtttcc
     4981 tgagctgttg agaaaagtcc ttaatgatat tttagaagat ataaaagatt cgcatcctat
     5041 ccaagatggc ctccctacac ctcccgaaga tatgccagat ggcggaccaa cccccggagc
     5101 caatgagaaa acatcccaac ctgtaattca ctatcatata aataatgata atagaactta
     5161 cgataataga gtttttgaca acagagtata tgacaatagc tatcacgaga acccagaaaa
     5221 tgatgcacag tctcctactt ctcagacaaa cgatctatta tcccgtaacg gaaactcatt
     5281 actaaatcca caaagagcac tagttcaaaa agtaacttcc gttctaccac actctatatc
     5341 agatactgtc cagacatttg caaataattc agctttagaa aaggctttca accatactcc
     5401 agataattcg gatggaatag gttcagacct gttaactacg agtagtcaag aaagatctgc
     5461 aaataactct ctttctcggg gacacaggcc tctgaacata cagaactctt caaccacccc
     5521 ccctctccac ccggaaggag tgacaagcag taatgataac tcatcagata caactaaaag
     5581 tagcgcttct ctttctcata gagtagcttc gcaaatcaat aaattcaact caaacactga
     5641 ttcaaaagta cttcagactg attttttatc aagaaatgga gacacatatt taacacggga
     5701 aacgatattt gaagcttcaa aaaaagtaac aaactcccta agtaatctta tatctctcat
     5761 tggaactaaa tcaggaacac aagaacgaga gttacaggaa aaatcaaagg acattacaaa
     5821 atccacaaca gaacatagaa taaacaacaa attaaaagtt acagatgcaa atataagaaa
     5881 ctacgtaaca gaaaccaacg cagatacaat tgataaaaat catgcgatct atgaaaaggc
     5941 aaaagaagta tctagcgccc tcagcaaggt attgtcaaaa attgacgata cctctgcaga
     6001 attacttaca gatgatatat ctgatttaaa aaataacaat gatattacag ctgaaaacaa
     6061 taatatatat aaagcagcaa aagatgtaac cacttcccta tcaaa
//
LOCUS       DAZPCS010000189         6085 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZPCS010000189 DAZPCS010000000
VERSION     DAZPCS010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6085
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.192"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..143)
                     /locus_tag="ACHUQ7_003587"
     CDS             complement(<1..143)
                     /locus_tag="ACHUQ7_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HFR1136827.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQ"
     gene            379..651
                     /locus_tag="ACHUQ7_003588"
     CDS             379..651
                     /locus_tag="ACHUQ7_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HFR1136828.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(617..1024)
                     /gene="nudG"
                     /locus_tag="ACHUQ7_003589"
     CDS             complement(617..1024)
                     /gene="nudG"
                     /locus_tag="ACHUQ7_003589"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFR1136829.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELDIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            1111..1758
                     /locus_tag="ACHUQ7_003590"
     CDS             1111..1758
                     /locus_tag="ACHUQ7_003590"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFR1136830.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWFAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(1767..3074)
                     /gene="ynjE"
                     /locus_tag="ACHUQ7_003591"
                     /pseudo
     CDS             complement(1767..3074)
                     /gene="ynjE"
                     /locus_tag="ACHUQ7_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
     gene            complement(3141..3794)
                     /locus_tag="ACHUQ7_003592"
     CDS             complement(3141..3794)
                     /locus_tag="ACHUQ7_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR1136831.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(3794..5265)
                     /locus_tag="ACHUQ7_003593"
                     /pseudo
     CDS             complement(3794..5265)
                     /locus_tag="ACHUQ7_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
     gene            complement(5238..6085)
                     /locus_tag="ACHUQ7_003594"
                     /pseudo
     CDS             complement(5238..6085)
                     /locus_tag="ACHUQ7_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         1427 a         1624 c         1611 g         1423 t
ORIGIN      
        1 atctggcgat attttggatt ttgttcaaga aaaggccaga gtgtggtcat tacttcacga
       61 acggcttgcg cgaactcggt ttgattcggg tcgcgctttt ggacatggtt gaggaatgac
      121 tccagagaat atgtctgatc catagatata aaaaccttat atattaatac gattgtgctt
      181 atgttgttta tgtcatgtgc ttttgcagtt ttcgactata ccatcaagaa tgtgacggta
      241 agcaagaaaa aaatgaccca ggaaagcaaa ttaataacga gagtaatctc ataaccaaat
      301 gcgataactc agacgctaaa gttttgccac ccggctaccg ttatcagtag cagagacaat
      361 aacgttaagg agttccctgt gagtcgagca ttgttcgccg tagtattagc atttccatta
      421 attgcactgg ctaacccgca ttatcgtccg gacgtggagg taaacgtacc gccagaagtg
      481 ttcagttcag gcgggcagtc agcgcagccc tgtactcagt gctgcgttta tcaggatcaa
      541 aattattctg aaggggcggt aattaaagcc gaaggaatac tgctgcaatg ccagcgtgat
      601 gacaaaaccc ttagcactaa tccgctgctc tggcggcgcg taaagccata aacgcctcta
      661 ataatggaat gtcagcaggg gccagcggat attgcagcgc ctcttcaggt gagcaccaga
      721 ccagcgcctg atgttcatgt gcctgtaacg tcccgtggaa gtcgggtacg tgccaggcat
      781 gaagatggat aatccggccc gaaacttctc gctgatggct ggcaacatac tcacccacag
      841 ttgcttcgat gtccaattct tcgtttaact cacgcaccag cgcctgctgc tggctttcat
      901 ccagctcgac tttacctccg gcaaactccc ataatcccgc ctgatcgctc tgggcggggc
      961 gttgcgcgag taaaattttg ccatcacgtt caatgatggc ggcaacaact tcaatcattt
     1021 tcataaatta cacacgggca gataagcgaa aaatgccata atatccgcac tgttgctcaa
     1081 tcagaaaggc tttatattta aggagtgcct gtgctagacc gccatcttca tccccggatt
     1141 aaaccgttgt tgcatcagtg cgtgcgggtt cttgataaac cgggcattac gccagatggt
     1201 ttaacattag tgggatttgc catcggcgtg ctggctctgc cgtttctggc gctgggctgg
     1261 tatctggcgg cgctagtcgt cattttgttg aacaggctgc ttgatggtct ggacggtgcg
     1321 ctggcgcggc gcagagggct taccgacgcg ggcggctttc ttgatatttc tctcgatttt
     1381 ctcttttacg cgctggtgcc gtttggcttt attcttgctg caccggagca aaatgcgctg
     1441 gcaggcggct ggttgttgtt tgcgtttatc ggcacgggca gcagttttct cgcctttgct
     1501 gcactggcag caaaacatca gattgataac cccggttatg cacataagtc gttttactat
     1561 ctggggggat taaccgaagg cacagaaacg atcttactgt ttgtgctggg atgtttattc
     1621 cctgcatggt ttgcgtggtt tgcgtggttt gcgtggtttg cgtggtttgc gtggatcttc
     1681 ggtgcgttgt gctggatgac gacctttacg cgggtgtgga gtgggtatct gacgctgaag
     1741 tcgctccagc gtcagtaatt gcaatgttat ttgctactgc ccgggccgcg ttcaccggtt
     1801 gcaaccggat tttttggatc gctgctccat tcgtaccagc cgccgtcata cacggaaaca
     1861 ttcttccaac ccatggcgcg tgcgtacata aaggtttcgg acgcgcgcca gccggtgccg
     1921 cagtagaatg aaacttgctg atctggtttg atattccatg ctttccacat agcggtaata
     1981 tcatcggcgc tacgcatggt gccatccggg ttatagaaat cttccatatg cgtcgagtcg
     2041 ctaccagcgt gtccccaacg tgctccggct atttcacctt ttggtttaat atagctgtaa
     2101 ccgctggtcg taccgataaa ttctggccac gaacgaatgc tcaccagcga tgcatcctgg
     2161 cgatgcagca gtccacgggc ctgttccata tcgagcatca actgcggttg tgccgggatc
     2221 ttcacgccga aatccggttc cgctttcact ttcggtggcg ttcctcgctc aacaggcagt
     2281 cccgcgtcgg accaggtttg ccagccgcca tccagcagac gcacatcttt cacgcctgcg
     2341 tagagcataa tttgcgccac acgcgctcca gcgtatacgt cacgcccgta cagaatgacc
     2401 gtggtgtcat ggcgaatgcc gtgttttgcc agcatcgctt ttagttgttc atcagaaact
     2461 ttgttccaca gcggttcgct ttccacttcg ttggtatcga tgtagtcagc gccaggaata
     2521 tggctgataa ggtaaagctt aggagcgccc taggccgctt caatgacttt ccagtcaccg
     2581 acaggtttcg ccgtaacctc tttaccttgt tgcaggtcat gcagccattg cggataaacc
     2641 agctgctcaa agtgcggcag tttttgtaga cgggaaggtt cgcttagcgc gtcactcaga
     2701 atggagatat gcgtaaaacc tgctttttgc agtcgcgttt tgacggcgtc aacatctttg
     2761 ccattaccgt acagcgccac cggggcatcg gctttcaggt tatgttgctt gatccacgcg
     2821 ttgagctgtt cggtgctcat tttgtccagc cagctagcag agaggtttaa ggcggcaggt
     2881 tcatgaccag aagggccatt taaggtttgt ggccagccgt tataaaacgc gctggggcga
     2941 gtatctatcg ctttgccatt ttgttgctga agttggtcaa gtgtaagagg cttcgccagt
     3001 tcagcggccc acgaagaagc gcaagccagc cctaaagcca ttgccagcgc ggtcatttga
     3061 gaaacacgtt tcatcgaaaa tcccgacatt gtagttttga gtgcgccatt ctgaactgac
     3121 gcattgagaa aaactttgcg ttatcgcagt ttgttgtaat tttctgacca ctgcgccata
     3181 tccagaacag aactatcagc aggaacatcc tggagatcgt gcgttacctg aacgacgggg
     3241 atcgccaggg cgcgaacttc gctgaacacc cactggcgaa aattatcgcg cagagccaca
     3301 tcaagacggc tgaatggctc atccaggagt aacgcttttg gctgggcgag aagggcgcgt
     3361 agtagagcaa cgcgcgctcg ctgaccgcca gacaaagtgg caggatcctg atggaaagct
     3421 ccgtcaaggc ctgaacgctc aagtgcatca ttcacggcat tacgtcgggc attcccctta
     3481 agtgtcgccg gtagcgccag cagtaaattt tgcccgacgc tgaactggtc aaataacagt
     3541 gcatcctgaa aaagaatgcc aatctgacgc tgtgcggtgg gtagcatgtc aatccgttgc
     3601 tcattgagcc atagctcacc tgtacaagaa aactgtccgg ccagtgcacc aatcatccat
     3661 gaaaacagag tggattttcc acagccagac ggccccatta acgtgacgat gtcaccttta
     3721 tcaaccgtaa agttaacgtt tgtcagcaag cggctttctg gtaaacgtag cgaaacattt
     3781 ttcacgcaga gcattagcgg attccttgtc tgacataacc tacccatttt gcgactaacg
     3841 cggtcagggc aaaaataata agcggtaaca acagttgcca taacgcctgg gcggcgagaa
     3901 taccgttgct gccgccgctg cttaatgcca ccgcctcagt ggtgagcgtc ggaaaacgcc
     3961 ccgcgcccag ccacagcgtc ggcatatact gcgcaatact gactgaaaat cccaccgcga
     4021 aggcaatcag cacagggcgc aacatgagtg ggcatttcac gtaaaagaag attttggccc
     4081 gcgaccagcc cagtgtttgc gcaatcaaaa ttaaccgtga atcaatgcgc tgccaggcgg
     4141 gttgcaggat aaacagcatc cacggcatta cccacagcag atgcccccag accaccgccg
     4201 tccagcttcc atccaggttc agccatagcg ccagcgtgta ctggcccgcc accagtggca
     4261 gagcgggcaa tagaatgggc aaccatagcc acaactggcg acgctgtgcc ccccattcca
     4321 gccacagcag taacaggagc aaagcgatga atgttgctac cagccccatt gtcagactgt
     4381 tgatgagtgc ttcactattg atcgtcgact gatccgcgag gatcgccagc agaaccacac
     4441 acagcacacc ggttaagggt aaaaaactcg ccagcgtagt gcctggtaat aaaggtgtgg
     4501 caggcttgcg aatgccatct acgcggggaa tagtgcgccg ccagctgcgc caaaacgtag
     4561 gcggcgagta acagcatcag cagcagactg gcgagtgcgc ctttcgtttg ttgatccgcg
     4621 tcgccctgag ttaaccactg ccagctaatt actgccagcg tcggtggatt acctggccca
     4681 agaataatcg ccacatccac gaccgaaagc gaccaggcaa caatcgccag catcgccatt
     4741 gccagcggct gtagcccagc gaatccagca caatgacctg ctgcaacagc cgtttttcgc
     4801 tcaatactgc cactaagatc cacagcagaa atgcgctttc tttcactgca agggtgaggc
     4861 ccagaccgat ccaaatcggt ccattggcgg agtgaaatac gggaaatagt catatagcag
     4921 ccctccgtca gcaaagagca gcagggcgct ggtggcaaaa gccacatggg gaatggcgag
     4981 cagccacggc agacgggcgc acatacgctg ccatttcggc ccaggccata gcgccacaat
     5041 caccagcagg gcaatcaaca atgccccgac cgccgcgatg gttgtcgaca ccagcgttgc
     5101 cagtaatgcc tgcggtaact gtggatcggc aaataacgcc tgccagcgtg tcaacgacaa
     5161 cgcaggcgat atcagcgtga gagctgccgg gatcagcggt gcataaatca ccgccaccat
     5221 cgcccacagc aaaaagatta atgcgtaccg taacggcgta accattcttg ttccagtgca
     5281 ttcacccatc ctgcgtgcgg ttcagccagt accggcggca gatcctgcgg cattcttgat
     5341 tgcaatattt cgcgctgccc gtcaggcagt ttttgcggat cgagaacaga aggatcgccc
     5401 cagacaacgg gatctgcttt acgcagttgc gcatcgggtg agagcaggaa attggcaact
     5461 accttcgccg cagcactggc attcgcgttg gctggaatgg tgacgaaatg cacgttgcca
     5521 atcatcccct cgcgaaagcc aaaactgtaa ctgcttgccg gcaaatcgcc gctggcgatt
     5581 ttttgctgcg catgtgcggg gttaaaggtc agcgacaggc gcaatgtgcc ggctttcagc
     5641 agagcatcca tccgcgcggg cgaaggcggg aaatcctttc cttcgcgcca caaatacgga
     5701 tgcagtgcat caagatattg ctacaagggc gcagtgacac gggcgaaagt cgcatcgtcc
     5761 ggcgcttctt ttaatgcggc gggatctggc gtcagcataa tcagcaactg ttcaagaaac
     5821 gccgtgccgg taaagtccgg tgggcgcgga taagtaaccg tgccgggatt ggctttagca
     5881 aactccagta aggcttgcgg cgtttgtggt ggctgtggcg taacatcgcg gcgggcgata
     5941 aacgtcagtt gtgcgccgcc ccagggcgat tccgcacctt gtgtcggcac tgaaaaatct
     6001 ccccgcaccg gcagctgtgt gtcgacatag cgccagttgg gcagagtctc cgcccagccc
     6061 gtttgtaata aattggcctc tttta
//
LOCUS       DAZPCS010000190         6061 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZPCS010000190 DAZPCS010000000
VERSION     DAZPCS010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6061
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.193"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            182..1006
                     /gene="gadX"
                     /locus_tag="ACHUQ7_003595"
     CDS             182..1006
                     /gene="gadX"
                     /locus_tag="ACHUQ7_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFR1136832.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTNRSAEGIRL"
     gene            1374..2102
                     /gene="gadW"
                     /locus_tag="ACHUQ7_003596"
     CDS             1374..2102
                     /gene="gadW"
                     /locus_tag="ACHUQ7_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFR1136833.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2465..5578)
                     /gene="mdtF"
                     /locus_tag="ACHUQ7_003597"
                     /pseudo
     CDS             complement(2465..5578)
                     /gene="mdtF"
                     /locus_tag="ACHUQ7_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
     gene            complement(5603..5860)
                     /locus_tag="ACHUQ7_003598"
                     /pseudo
     CDS             complement(5603..5860)
                     /locus_tag="ACHUQ7_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
BASE COUNT         1597 a         1426 c         1436 g         1602 t
ORIGIN      
        1 tttcactgcg ttcttaaaca cgtttttgta ttataaatcc agtcatcctg cccgtagaac
       61 atcacacatt atcatcctgt tctcccgctt cgtttaaatt tatttatcaa tcaatttgac
      121 ttaagagggc ggcgtgctac attaattaac agtaatatgt ttatgtaata ttaagtcaac
      181 tatgcaatca ttacatggga attgtctaat tgcgtacgca agacataaat atattctcac
      241 catggttaat ggtgaatatc gctattttaa tggcggtgac ctggtttttg cggatgcaag
      301 ccaaattcga gtagataagt gtgttgaaaa ttttgtatta gtatcaaggg atacgctttc
      361 attatttctg ccgatgctca aggaggaggc attaaatctt catgcacata aaaaaatttc
      421 ttcattactc gttcatcact gtagcagaga tattcctgtt tttcaggaag ttgcgcaact
      481 atcgcagaat aagaatcttc gctatgcaga aatgctacgt aaaagagcat taatctttgc
      541 gttgttatct gtttttcttg aggatgagca ctttataccg ctgcttttga acgttttgca
      601 accgaacatg cgaacacgag tttgtacggt tatcaataat aatatcgccc atgagtggac
      661 actagcccga atcgccagcg agctgttgat gagtccaagc ctgttaaaga aaaaattgcg
      721 cgaagaagag acatcatatt cacagttgct tactgagtgt agaatgcaac gtgctttgca
      781 acttattgtt atacatggct tttcaattaa gcgagtcgca gtgtcctgtg gatatcacag
      841 cgtgtcgtat ttcatttacg tctttcgaaa ttattatggg atgacgccca cagagtatca
      901 ggagcgatcg gcgcagggat tgccgaaccg tgactcggcg gcaagtattg ttgcgcaagg
      961 gaatttttac ggcactaacc gttctgcgga aggaataaga ttatagagtt ttactcagac
     1021 ataaaaaaaa cccggcatag gggaccggga agaggatagt ctgccgtctc cagactaata
     1081 aaccgttata acactccctg ttggcacggg aaactttgtg ctctcagtaa gttaaatata
     1141 acttttacag gaaataagat cagccatttt tttataaaca taagctatac gctgtgcgaa
     1201 aatatattcg tgctgcattt acttattatc aattaactgt tatgcaaaac tactttgtgg
     1261 ataaattttg gtcctaccaa atctggcagt ttttgcgcta agaaacagtc tgtcatcatt
     1321 tcattagtat actgaaattg aaataatcgc agtatgaaat ataagggata atcatgactc
     1381 atgtctgctc ggtgatcctc attcgtcgtt cattcgatat ttatcatgaa cagcaaaaaa
     1441 tatcgctgca taacgagagt atcctgctgc tggagaaaaa tttggcagac gattttgcgt
     1501 tttgttcacc ggatacgcga cgactggata tcgatgagct gacagtttgc cattacttac
     1561 aaaatattcg tcagctacca cgcaatttag ggttacatag caaagaccgt ctgttaatta
     1621 accagtcacc ccccatgccg ctggtgacgg cgatttttga tagcttcaat gaatccgggg
     1681 taaattcacc gatactgagc aatatgctct acctttcctg tttatcgatg ttttctcata
     1741 agaaagaact gatcccctta cttttcaata gcatcagcac tgtttcagga aaagttgaac
     1801 gccttattag ctttgatatc gccaaacgtt ggtatctgcg cgatatcgcg gaaagaatgt
     1861 ataccagcga gagtctcatc aaaaaaaagt tgcaggatga aaatacctgt ttcagtaaaa
     1921 tattactcgc ttccaggatg tcgatggcca gacgattact cgagttacgt caaattcctc
     1981 tgcatactat tgcggaaaaa tgtggctata gcagtacgtc gtactttata aacacatttc
     2041 gacaatatta tggtgtaacg ccacatcagt ttgcgcaaca ttcgccaggt accttttcct
     2101 gacatatttt gcatttgaat attggtcagg atctcacacc tgcttcatgt gaaactcttc
     2161 cctgatgatt tctgccgggc taccggctag ttctctttcg cagtttgcca ggcggtatag
     2221 gtatttttag gaggaaaaga ctcggcatgt ttgggattat taagctgaca attcatacga
     2281 ttacgggaat gtgggtcagt attgtgttgt tcaaattgat gacgaatggc tggagtggtt
     2341 tctactttca gtgctgtgta ctaagcctgg tattcctgac ggttagctgg ttgttgagtg
     2401 gtgaatggtt agcaggaaag agtaaggctg aaccttcacg ttcaacctta ctctcattta
     2461 cacgttacgc ttttttaaag cgggcaaaga gatgttccac tacaacgaaa aagaccggaa
     2521 cgaagtaaat tgccagcact gttgcggcaa acatcccgcc catcacgccg gtacctaccg
     2581 cgttttgcgc gccagaaccg gcaccatgac tgataaccag cggcagcacg ccgagaataa
     2641 aggccagaga ggtcatcagg attgggcgta aacgcatccg cgccgcttcg atgattgcct
     2701 ctatcggcgt tttcccttct ttctgcatca tctcaacggc aaattcgaca atcaggatgg
     2761 cgttcttggc ggaaagcccg atggtggtca gcaaaccaac ctggaagtag acgtcattac
     2821 ttaagccgcg cagatcggtg gccagtaatg cgccaacgac gcctaacgga acaaccaaca
     2881 tcaccgagaa cggaattgac cagctctcat agagtgcggc gagggcgagg aacaccacga
     2941 ccagtgaaat ggcatacagt gcaggagcct ggtttgagga taacgcttcc tgatacgaca
     3001 gtccggtcca tgagtagccg acgcctgccg gaagtttagc gaccaggtct gccataaatt
     3061 tcatagcgtc accggtactt ttcccggccg ccgcttcacc taaaatctcc attgacggga
     3121 tgccgttgta gcgttccaat cgcggtgaac cataggtcca ttcggtagac gagtaggcag
     3181 aaagcggtgc catcgtgcca gaggcgttgc gtacatacca ttggttgatg ttatccggca
     3241 acatacggaa cggcgtgcct gcctggacat acactttttt cacccgcccc tggttgagga
     3301 agtcgttcac gtagctgctg ccgaaggcgg tggaaattgt ctggttgata tcagacagcg
     3361 ccacacccat tgcctcagct ttcgcggcgt tgacgttcac tttgaacatc ggcgtatctt
     3421 ccaggccgtt cgggcgtacc ccggtgacct ggtcaggcga ttgcgctgcc agtgataaca
     3481 gctcgtttcg tgcctaggtt agtttttcgt gccccaggtt accgttgtcc agcagttcca
     3541 tatcaaaacc tgacgcggta cccagttcag ccaccgcggg taagttgaac gggaagacga
     3601 cggctttatt gatactgctt aacgcaatca ttgcccgctg aatgatcgcg gtaaccgagt
     3661 tttcctcacc gacacgttca gaccacggct tgagactgat aaacgccagg ccgttgtttt
     3721 gcccctggcc gctgaagcca aagccgccaa cggtaaacac cgactgaaca ttatctttct
     3781 cttttgtaag ataataatcc gtcacctgtt gcagtacttt cgtggtgtta accatggtgg
     3841 caccggaagg taactgcgcg gtggtcataa ataccccctg atcctcttct ggtaagaaag
     3901 aggtcggtgt acgcaggaac agcaccgcca tcccggcgca aatcagcagg tagaccacca
     3961 tgtagcgacc ggtacaacgc aacagcgagc gggtgctatc ggtatagtgc tgagttgatt
     4021 tttcaaacag cgtgttgaag cgtgcgaaca gggcgttagg tttgtgaccg ccttccggcg
     4081 tggctttcag aatggtggcg cacagggcag gggtcaggct cattgccaca aatactgaaa
     4141 gccgcatgga ggagatcagc gtgatggaga actggcggta gatctccccg gttgcaccgc
     4201 tcataaaggc catcggcata aacactgcag aaagaacaac ggcaatcccg accagcgcac
     4261 gttggatctg ccccatcgat ttatgcgtcg cttccttcgg cggtagctta tcttccgcaa
     4321 tgacacgctc gacgttctcc accaccacga tggcgtcatc caccagtaac cctatcgcca
     4381 gcaccatccc gaacatagtc aacgtgttga tggtgaaacc gaccgccgac aagatcgcaa
     4441 acgtcccgag aataaccacc ggtacggcaa ttgtcgggat gatggtggca cggaaattct
     4501 gcaaaaacag ataaatgacc aggaagacga ggatgatagc ctcaaccagt gttttgaaaa
     4561 cttcctgaat agaaatttcg ataaacggcg tggtgtcgta aggataaacc gtcttcagac
     4621 ttgcggggaa ataggctgat aagcggttca gctcctcttt gactgcccgc gaggtatcca
     4681 gggcgtttgc tccggcagcc agtttgatgg cgatcccggc aacaggtttg ccgttatagc
     4741 gcgccacggt ggaataatct tccgccccaa gttcgacgcg ggcgacatca cgcagcagca
     4801 cttgcgaacc atcttgctga actttcaaca ggattttgcc aaattcttcc ggcgtttgca
     4861 gacgcgtctg cacaatgatc gaggcgttta gttgctggtc tgccgcctgt ggcatgccac
     4921 ccagttgacc accagaaatc tggttgtttt gcaccttaat ctgggaaata acatcggaag
     4981 gtaccaggtt atatttattg agtttttgcg ggtccagcca gatacgcatg gcgtactcgg
     5041 aaccaaagag ctgtacgcta ccaacgcccg cggtacggct tagcgggtct ttgatattag
     5101 acgctacata gtccgcgata tcgtactggt tgaggctgcc gttatcagaa ataaacgccg
     5161 ctaccatcag gatattactg ctcgacttat cgacgctaat cccctgttgc tgcaccgctt
     5221 caggtaatga aggcatagcg agttgcagtt tattttgcac ctgaacctgt gcgatatcag
     5281 gagatgcccc agtctcgaag gtcagagtga tagaggcatt acccgccgca tcactggttg
     5341 aagacatgta catcaggcca tcaagcccat tcatattttg ctcaatcacc tgagtgaccg
     5401 agtcttctac cgtttgcgca tcggcacccg gataggtagc gctgacggta atggtcggtg
     5461 gcgcaatctg cggatactgc gcaaccggta agttcatgat cgccagacca cctgcaagca
     5521 tcataataat ggcaagtacc caggcaaaaa ccgggcgatc aataaaatag ttagccatga
     5581 aagtcccctt aagcctgcaa cgttattgtt tcgattcggt gctggcgttt tcctggctgg
     5641 aggaaattgc tcgtgctttg atacccggac gaatgcgttg caaaccggaa acgatcaccc
     5701 gatcgccagc ctgcaagcca gaggtgacaa cccactggtc gccgatggct ttgctggctt
     5761 caatttcgcg tagctgcacg acatcgtctt tatccagaat gagcgccgtt gctttaccct
     5821 gggcgttgtg ggtgacgcct tcctgcggca ccagtaatat aaagcgcgca aaattattca
     5881 agatattttt gaaaatcatt caagataggc tatcgcgtgt gatagcaaaa acattcaaga
     5941 taaaaatgct tgttaattaa caaatgatat cttctatata tttttattgt ttatagatgg
     6001 ctatttcggt aaaaatgcat tttcagaata tcaaccagcg gtaaatcgaa gtaaagggag
     6061 t
//
LOCUS       DAZPCS010000191         5984 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZPCS010000191 DAZPCS010000000
VERSION     DAZPCS010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5984
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.194"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(118..436)
                     /locus_tag="ACHUQ7_003599"
                     /pseudo
     CDS             complement(118..436)
                     /locus_tag="ACHUQ7_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842854.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(580..3888)
                     /gene="icsA"
                     /locus_tag="ACHUQ7_003600"
                     /gene_synonym="virG"
     CDS             complement(580..3888)
                     /gene="icsA"
                     /locus_tag="ACHUQ7_003600"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HFR1136834.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFATPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSVINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEIAGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYSWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTAHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGGNNIQAKLGLRTSLISQSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            4417..5619
                     /gene="virA"
                     /locus_tag="ACHUQ7_003601"
     CDS             4417..5619
                     /gene="virA"
                     /locus_tag="ACHUQ7_003601"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HFR1136835.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDILLKIITFG
                     IYSPHETLAEKHSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFHVKDTDFNSVWHDIYRDIRASDSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQVNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
     gene            5798..>5984
                     /locus_tag="ACHUQ7_003602"
     CDS             5798..>5984
                     /locus_tag="ACHUQ7_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR1136836.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQGEYDSQWAAICSIAPKIGCIL
                     ETLRVWVRQHERDTGGGE"
BASE COUNT         1840 a         1355 c         1018 g         1771 t
ORIGIN      
        1 ttcaggctgt acgcttcccc atgtgatttg cctcgccact tcaccgggct caggaccccg
       61 taggatcttc accgaaaaaa atgcatcacc agttgcttta ttatttgttt taggtagacc
      121 tgttactaat ttatacctac tctgaccaag tctggataag aaaggcacac ctgcacattt
      181 ctacttggaa atgttttctt ttaccatttt gctcaaaaat ttcaacatcc aatattcctc
      241 gtacagaact actcaagtcc tgaatagaaa aagttccagc tggtacagta gagtcatata
      301 ttactcgacc ttgttgagat acaacaacac gtgcattagt ttctgcaata ccagtaatct
      361 gaggcgcata accacgaata tttggcggta gcattcgttc atcgctttct aatgcaattc
      421 ctgtgtaacg ccaagcaaca gcgcattctg gcgcatgatc tggtaaacac gtttggcatt
      481 gatcgcaggc ataccatcat gtgcacaaaa acccaccgag cttttacacg gtgggtccca
      541 gagaaatgca ggacatcaac acgccctgca tttttattat cagaaggtat atttcacacc
      601 caaaatacct tgggtgtctc tgtaactgtt attacccaat tgctgagata cgtttcccca
      661 gatgcttagg ttatctgcaa gacgcccccc cacaccagtt ttgagttcaa tcacattacg
      721 gttgcctatc tggtgatttg acatgccatt cataattaca ccatattgct ttgagctcca
      781 tttccaattc acttccaaaa atggttcgaa cttacgaaga gtctccttat cgatacaact
      841 ctgactaatt aaggatgtac gcaatcccaa ttttgcttga atattattgc ccccagagga
      901 tgtgaccata gtgccattgt gttcaatata gtcgtttgct tttaccccat gcctagttat
      961 ctggacttgt ggattgagat acaatgcgtt gtcaatatta ttatgagcag tccagcgtat
     1021 ggttgggtag atatagccag cttccaaagc tcctgttatt cctttgctgg aatatttctc
     1081 accagttaac ccatctcctt tgactgtatt attaaaccaa ctgtactgca accaagcatc
     1141 catatataga cctgttcgtt ctttttcatc ctgaaaccaa gaactgtaca aaccggcagt
     1201 ataaccgctc acagttcctc gtgaactata actagtatac ttattatgag ttttgctatc
     1261 ctgatatcca taagctccca taatacccac atgcaagcca tcatgatcag tgaagtttgt
     1321 cttcaaaata tctccaccaa tctgataaac catcctgtta gctgtggttt ttaattgtct
     1381 atcagctaaa ttatgctcat tatgtccacc aacagtacga atccacatgg tggtttcgct
     1441 gagctgacct gtagtaggat caacaatagt gctaccgtgt cggtcataga gagaaggact
     1501 aaacatcgtg ttcattgcta tcaggttagc aatataactg ccagcttcag ggcgccatgt
     1561 gtgaatacta ccttcaccca aattaagtga agatacaagc tgtgaactag ctcggcgact
     1621 actcatttga gtagactctt gattgctcat ctgttttgat tcttgattat ccatctgact
     1681 agttagatac cacttattgg tattcagtcc agatacagtg ccctgtttca ggcgatagtc
     1741 ataactacca gcaacaatac ggcctttctg aataaaagca ttcttatcag aagaatctgt
     1801 cgaaataatt tgaacacctt caagagtttt atctccagag cctccctcat ttgtcactgc
     1861 aacataggta ataccagacg catggcctgc aatttccagc cggtcagttt tactgttgtc
     1921 attgccgaga accgcgccca aagataatat tccgcccttt ccatgccaat tacctttctg
     1981 aacataggtc tgaccgacat ttgacgaact attattgaga ataacatgac cgtttttctc
     2041 cagagtcatg ttacctgtga caatgacggg gtcaggcaaa aaaggggcat taatattatt
     2101 gattagcaca gtcccactat ttaataaagt gttaacagtt tggttcatgc ctgaaaaatt
     2161 caaggtagca cctttattaa caataacacc agcggtacgt gtcatagctt caactgtccc
     2221 catttttaga atgcctgcat tgatgttggt gaaaccagag taggtatttt tttccgccaa
     2281 aatgagagtt ccctcccctg attttgttag ggattttccg tcccagctat ttatattatg
     2341 atggctgaga ttatctgcca gaatagtact aacagtaagt tcagcgtttt ctttcagatt
     2401 gaactctcca tgactgtcgc catctgtatc attccagctc aaaccgtaga tcaatttatt
     2461 ctggtcactg atatagcttt ttgtatagtc aatagcacca gtagtgagta acggattgat
     2521 gatagatata ttagaaacac tgaaacctgt tatcccttta tctgcactta gcagtttata
     2581 gtccttctgc gcccagttat ttataatagc ctccactttt acattgttgc caaatgacat
     2641 atttccagaa gcttccagac tactgattcc agctaaattt aaagaactgt tctcaccaaa
     2701 agaaacgtca cctgaaacag tgaatttgtt acctgatagt gaaacagttg tacaatcacc
     2761 agcacataaa tcaccttcaa tagttggtgt ggtcccagtg taatcattgt tgataatttt
     2821 cagaatggat gaattgttaa cctgtacatc accagtaatg acagaacctt catggagtat
     2881 taatgaattg tttccaccag tgatgtggat agcattacct gctacatttt gtgttccatc
     2941 atcttcttta cctgaaatta tatatccgct attagcaatg gtcatattgc taccatttat
     3001 agcatccccc caatgtccac cattaccgcc taaaatagct ccattgttga ttacagacag
     3061 gttatctcct gtgatagcat taccaccgta gccatcataa ccataaccgt aagcagcacc
     3121 tccgttgcct ccagcgaacg tgcccttatt gattacagag aggctgcttc ctgtgatggc
     3181 atcacctcca ttaccgccat caccctcacc ataattgtta cctccattac caccggaaat
     3241 agtccctttg ttgattatgg gtaaattgac tcctgtgacg gcatcacctc cattcccccc
     3301 atcactatca ctataactat cgccaccatg tccacctgaa ataatttctc catttattat
     3361 aaacagattg tctcctgtaa cagcctcacc accatcaccg ttatggtcag caccgctacc
     3421 gccgctacca ccaagaatca tgccttgatt gattatagac aagtcagatc cggtaataga
     3481 gtcaccacca ttaccgccac aactattacc atcattatta tcaccagcac caccatgacc
     3541 tgcgctaatc ataatatcag agttaatccg tagcttagtt ctagatgcat gagaggggtt
     3601 ggaagaagtt atataccacg catccattcc atcttcacca gctcccaagg gagaaagtcc
     3661 atcaacaggt gttaataatt tttcataatt gtcctctgaa aaatgaagtt cttgagtacc
     3721 cgaaagagga gtagcaaaag ctattggccc cccgagcaac agggatgcac caacaacaaa
     3781 aggagaaaaa gacaatttgc atgttttttc ctttaccgta ggtaattctc cggccccccc
     3841 ctgtgaacat tgggtcatat tacaaaaaaa tttgtgaatt tgattcatgc actatattat
     3901 cagtaagtgg ttgataaacc cctgaaaaga ttccgtttta tccggaataa agggacgata
     3961 tatgcaaaaa catattaaac aaagcctcaa ccaaaacatg agagctgtgt tatatatcaa
     4021 tgcaacggga gaaatttacc tgtgtcttgc tttgaaaaaa agagggggaa agctatatgt
     4081 tcgataataa attaataatt ttactatgat agtctgcttc agaccgcaac ttttcagttc
     4141 tttctgtacg cttgcccaaa gtttaaccac tttgtttttc tatacaccca atggttctat
     4201 ctgccccttg atgttaatcc ccaaagccaa taacatgaat gtattcatat cacaactttc
     4261 ctaccagact ctccaatgca ataactgcat aattgttttt agtggaaatg tatcgttttc
     4321 ttaaagagaa gaataacatt ccattttatt atgttcccaa tttaattata ttaatatata
     4381 ttggcattaa taggaaaata catcaggaga aatcaaatgc agacatcaaa cataactaac
     4441 catgaaagaa atgactcatc atggatgtca actgtcaaat caactacaga agtaagttgg
     4501 aataaattga gtttttgtga cattttactg aaaataataa ctttcggcat atattctcca
     4561 cacgaaactc tagccgaaaa acatagcgaa aaaaaactaa tggatagttt ctcaccatca
     4621 ctatcacaag ataaaatgga cggagagttt gcccacgcaa acatagatgg catttcaata
     4681 cggctatgcc ttaacaaagg catatgttcg gttttttatc ttgatggaga taaaatacaa
     4741 tccactcaac tttcatcaaa agaatataat aacttgttaa gttcactccc ccccaaacaa
     4801 ttcaacttag gaaaagtaca tactattaca gctccggtat cagggaattt taaaactcac
     4861 aagcctgcac cagaagttat tgaaacagcc attaattgct gtacatcgat aataccaaac
     4921 gatgattact tccacgttaa agacactgat tttaatagtg tttggcatga tatttacaga
     4981 gatattcgag catctgacag caatagtaca aaaatatatt tcaacaatat tgaaatacca
     5041 ctaaaattaa ttgcagatct aattaatgag ttaggaatta atgaattcat tgatagcaaa
     5101 aaagagcttc aaatgctaag ttacaaccaa gtaaataaaa taatcaattc aaacttcccc
     5161 caacaagacc tatgttttca aacagaaaaa cttttgttta cttcactctt tcaagaccct
     5221 gcatttatat ctgcactaac atctgcattc tggcaatctc ttcacatcac gtcttcctct
     5281 gtggaacaca tatatgccca aattatgtcc gaaaacatag aaaacaggct taatttcatg
     5341 cctgaacaac gagttattaa caattgtgga catattataa aaatcaacgc agttgtccct
     5401 aaaaacgaca ctgcaatctc tgcctctggg gggagggctt acgaagttag ctcatcaata
     5461 ttaccatcgc acataacatg taatggtgtt ggtattaata aaatagaaac aagctatctc
     5521 gttcatgctg gaacacttcc aagcagtgaa gggcttcgca atgctatccc tccagaaagt
     5581 cgtcaagtat catttgccat catatctcct gatgtttaag ccaatattat attggctgcc
     5641 agactgtaaa aaaacgttct tcagaacagc tctataaact attaaaccaa agctaaaagg
     5701 atggggacca atagaatctc agcccatcct ttttatatca ttatgaaccg ccccggaaat
     5761 cctggagact aaactccctg agaaagaggt aaacaggatg actaaaaata cacgtttttc
     5821 ccctgaagtc cgtcaacggg cagttcgtat ggttctggaa agtcagggcg aatatgactc
     5881 acaatgggca gcgatttgtt ccattgcccc aaagattggc tgtatactgg aaactctgcg
     5941 tgtctgggta cgccagcatg agcgggatac cgggggcggt gagg
//
LOCUS       DAZPCS010000192         5899 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZPCS010000192 DAZPCS010000000
VERSION     DAZPCS010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5899
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.195"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            1..411
                     /locus_tag="ACHUQ7_003603"
                     /pseudo
     CDS             1..411
                     /locus_tag="ACHUQ7_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            443..1057
                     /gene="istB"
                     /locus_tag="ACHUQ7_003604"
                     /pseudo
     CDS             443..1057
                     /gene="istB"
                     /locus_tag="ACHUQ7_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc10 family helper ATPase
                     IstB"
     gene            complement(1226..5320)
                     /gene="sepA"
                     /locus_tag="ACHUQ7_003605"
     CDS             complement(1226..5320)
                     /gene="sepA"
                     /locus_tag="ACHUQ7_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SepA"
                     /protein_id="HFR1136837.1"
                     /translation="MNKIYYLKYCHITKSLIAVSELARRVTCKSHRRLSRRVILTSVA
                     ALSLSSAWPALSATVSAEIPYQIFRDFAENKGQFTPGTTNISIYDKQGNLVGKLDKAP
                     MADFSSATITTGSLPPGDHTLYSPQYVVTAKHVSGSDTMSFGYAKNTYTAVGTNNNSG
                     LDIKTRRLSKLVTEVAPAEVSDIGAVSGAYQAGGRFTEFYRLGGGMQYVKDKNGNRTQ
                     VYTNGGFLVGGTVSALNSYNNGQMITAQTGDIFNPANGPLANYLNMGDSGSPLFAYDS
                     LQKKWVLIGVLSSGTNYGNNWVVTTQDFLGQQPQNDFDKTIAYTSGEGVLQWKYDAAN
                     GTGTLTQGNTTWDMHGKKGNDLNAGKNLLFTGNNGEVVLQNSVNQGAGYLQFAGDYRV
                     SALNGQTWMGGGIITDKGTHVLWQVNGVAGDNLHKTGEGTLTVNGTGVNAGGLKVGDG
                     TVILNQQADADGKVQAFSSVGIASGRPTVVLSDSQQVNPDNISWGYRGGRLELNGNNL
                     TFTRLQAADYGAIITNNSEKKSTVTLDLQTLKASDINVPVNTVSIFGGRGAPGDLYYD
                     SSTKQYFILKASSYSPFFSDLNNSSVWQNVGKDRNKAIDTVKQQKIEASSQPYMYHGQ
                     LNGNMDVNIPQLSGKDVLALDGSVNLPEGSITKKSGTLIFQGHPVIHAGTTTSSSQSD
                     WETRQFTLEKLKLDAATFHLSRNGKMQGDINATNGSTVILGSSRVFTDRSDGTGNAVS
                     SVEGSATATTVGDQSDYSGNVTLENKSSLQIMERFTGGIEAYDSTVSVTSQNAVFDRV
                     GSFVNSSLTLGKGAKLTAQSGIFSTGAVDVKENASLTLTGMPSAQKQGYYSPVISTTE
                     GINLEDNASFSVKNMGYLSSDIHAGTTAATINLGDSDADAGKTDSPLFSSLMKGYNAV
                     LRGSITGAQSTVNMINALWYSDGKSEAGALKAKGSRIELGDGKHFATLQVKELSADNT
                     TFLMHTNNSRADQLNVTDKLSGSNNSVLVDFLNKPASEMSVTLITAPKGSDEKTFTAG
                     TQQIGFSNVTPVISTEKTDDATKWVLTGYQTTADAGASKAAKDFMASGYKSFLTEVNN
                     LNKRMGDLRDTQGDAGVWARIMNGTGSADGDYSDNYTHVQIGVDRKHELDGVDLFTGA
                     LLTYTDSNASSHAFSGKNKSVGGGLYASALFNSGAYFDLIGKYLHHDNQHTANFASLG
                     TKDYSSHSWYAGAEVGYRYHLTKESWVEPQIELVYGSVSGKAFSWEDRGMALSMKDKD
                     YNPLIGRTGVDVGRAFSGDDWKITARAGLGYQFDLLANGETVLQDASGEKRFEGEKDS
                     RMLMTVGMNAEIKDNMRLGLELEKSAFGKYNVDNAINANFRYVF"
     gene            5722..>5899
                     /locus_tag="ACHUQ7_003606"
     CDS             5722..>5899
                     /locus_tag="ACHUQ7_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1136838.1"
                     /translation="MLTRKSIDTVLLSVGAEKLSQREWDWMKMLKPMDPPPAMVAASI
                     LERRGDTAALTRLQD"
BASE COUNT         1445 a         1488 c         1409 g         1557 t
ORIGIN      
        1 gccgtcaggc agcagcgaag caaagcgtta ctgacgtcgc tgaatgaatg gatggtggag
       61 aagaatggta cgctgtcgaa aaaatccaga ctgggcgaag cgttcagcta tgtactgaat
      121 cagtgggatg ccctctgtta ttacagtgat gacggtctgg cggaggcgga caataacaca
      181 gcagaaagag cgcttcgtac cgtctgtctc gggaaaaaga attacatgtt cttcggcagc
      241 gatcatggag gcgatcgcgg agctctgctg tagggactaa tcgggagctg tcggttgaac
      301 ggtatcgatc cagaagcgta tctgcgccat atcctgagcg tactgccgga atggccttcc
      361 aaccgagttg acgaactgct gccatggaac gtagttctca ccgataaata agcgtcaata
      421 cggccttccg ttgacgctta cagaactcct gtggcgcgag agtgaaaaac tgcgtcgtta
      481 taaaaaggag gcccggttgc cagttgccaa aacgctaagc gaatacgact tcattcagtt
      541 gccggaactg aatggagctc agttccagca actctgtgaa acgacagact gggttgatgc
      601 tggagaaaac gttctgctgt tcggggccag cgggttgggg aaaagccatc tggcggcagc
      661 gatcgtggat ggcgtagtag gccagggcta ccgggcccgg ttctacagcg caggagagct
      721 gttgcaggaa ctacgtaaag ccagagctca gttgaaactg aatgagctgc tactgaaact
      781 ggatcgctac cgggtgatag tggtggatga tcttggctat gtcaaacgcg acagcgccga
      841 aacgggtgtg ctgttcgagt taatagcgca tcgctatgaa cgtgggagcc tggtgataac
      901 cagtaaccat ccgttcagca tgtggggcag catcttcgtg gatgagacta tggcggtggc
      961 ggcagcagac cgactgatcc atcacggata tatgttcgaa ctgaaagggg aaagctacag
     1021 gaaaaagaca gcgaaggcag taacaagcgt gacttgatgt cgcactgaag gggcggccag
     1081 tatagttggc gccagtcggc aaaactagtt gacgtctaat acctggacgg tcacttctga
     1141 gaaaaggcat ttacacagaa tcttaaacag gctcacggag ttctttcaga aaaaggcctg
     1201 ccgaatggca gacctatccc attcgtcaga aaacataacg gaagttggcg tttatcgcat
     1261 tatccacatt atatttcccg aacgctgatt tctccagctc cagtcccaaa cgcatattat
     1321 ccttaatttc cgcattcatc cctaccgtca tcagcatcct gctatctttt tcaccttcga
     1381 aacgtttctc tccggaagca tcctgcagaa ccgtttctcc gttcgccagc aggtcgaact
     1441 gataacccag cccggctcga gctgtgattt tccagtcgtc tccggagaag gctcttccca
     1501 cgtcaacacc agtacggcca atcagtgggt tataatcctt gtctttcatg ctcagagcca
     1561 ttccccggtc ttcccagcta aaagcttttc ctgatacaga accgtaaacc agctctatct
     1621 gtggctccac ccaggactct ttcgtcaggt ggtaacgata accaacttca gcaccggcat
     1681 accaggaatg agagctgtag tcttttgttc ccagtgaggc aaaattcgcc gtgtgctgat
     1741 tatcatgatg gagatattta ccaatcaggt caaaataagc tccggaatta aagagtgcag
     1801 aggcatacag accgccaccc acggatttgt tttttccact gaatgcgtgg ctgcttgcat
     1861 tgctgtccgt ataggtcagc aatgcccccg taaataaatc cacaccgtcc agctcatgct
     1921 ttctgtcgac accaatctga acgtgagtgt agttatcgct gtagtcacca tctgccgaac
     1981 cggtaccatt cattatgcgt gcccagacac cggcatcccc ctgagtatcc cgcaggtcac
     2041 ccatacgttt gttcaggtta ttgacctctg taaggaagga cttataacct gatgccataa
     2101 agtcttttgc ggctttcgag gctccggcat cagcggtagt ctgatatcct gtcagcaccc
     2161 attttgtggc atcatccgtt ttttccgtgc tgattactgg cgtaacgtta ctgaaaccaa
     2221 tctgctgcgt accggcagtg aacgttttct catcactccc tttcggtgcg gtaattaacg
     2281 tcacgctcat ttcactggct ggtttgttta aaaagtcaac caggacgcta ttattactgc
     2341 ctgacagttt gtctgtgaca ttcaactggt cagcccggct gttgttggta tgcatcagga
     2401 acgtggtatt atctgcagaa agctctttta cttgtaaagt ggcaaagtgt ttcccgtccc
     2461 ccagttcaat tcgcgagccc ttagccttca gtgcaccggc ctctgatttt ccgtcagagt
     2521 accacagagc attgatcata tttaccgtac tctgtgcccc cgtaatactg cctctcagaa
     2581 cagcgttata tcccttcatt aaggagctga ataacggaga gtccgtcttc cctgcatcag
     2641 catcactgtc tcccagatta atggttgccg cagtggttcc tgcatgaata tccgaactca
     2701 gatagcccat atttttaaca gaaaagctgg cgttatcttc gaggttaatc ccttctgttg
     2761 tagaaattac gggtgagtaa tacccctgtt tctgcgcaga aggcatcccc gtcagggtca
     2821 gagaagcatt ttcttttaca tccacagccc cggtgctgaa aataccgctc tgagccgtaa
     2881 gttttgctcc ttttccgagg gtcaggctgc tgttgacaaa gctgccaaca cggtcaaaaa
     2941 cagcattctg agaggtcaca ctcacggtgc tatcataagc ctcaatacca ccggtgaatc
     3001 tctccatgat ttgcagggat gatttatttt ccagcgtaac atttccgctg taatcactct
     3061 ggtcaccaac tgtggttgca gtggcactcc cttcaacaga ggagaccgca ttaccggttc
     3121 cgtcactcct gtcagtaaag acacggctac ttcccagaat gactgtactt ccattggtgg
     3181 cgttaatatc tccctgcatc ttgccgtttc tggacagatg gaatgttgcc gcatcgagtt
     3241 tcagtttttc cagcgtaaac tgacgggttt cccagtcgct ctggctggaa gaagtcgtcg
     3301 ttccagcatg aataactgga tgaccctgga atatcagcgt gccggacttc ttggttatcg
     3361 aaccttctgg caggttaact gaaccatcaa gagccagtac atccttacct gagagctgcg
     3421 gaatattcac atccatattg ccgtttagtt ggccgtgata catataaggc tggctgctgg
     3481 cctcaatttt ttgttgcttc acagtatcaa tggctttatt acgatctttt ccgacattct
     3541 gccagacact gctgttgttc agatcggaga agaacgggga atatgaactc gctttcagga
     3601 tgaaatattg cttggtagaa ctgtcataat ataggtctcc cggcgctcct ctccccccaa
     3661 aaatactgac agtattaacc ggtacattga tgtcactggc ttttaatgtc tgaaggtcga
     3721 gtgttaccgt tgattttttc tcactgttat tggtaatgat ggccccgtag tcagccgcct
     3781 gaagacgggt aaaggtcaga ttattaccat tcagttccag acgtcctcca cggtatcccc
     3841 acgaaatatt atccggatta acttgctgtg agtcagaaag cacaactgtc ggacgaccgc
     3901 tggcaatacc aacagagctg aaagcctgta cctttccgtc agcatccgcc tgctggttga
     3961 gaataacggt accgtccccc accttgagtc cgcctgcatt cacaccggtc ccgtttactg
     4021 tcagggtacc ttcgccagtt ttatgcaggt tatcaccggc aactccgttc acctgccaca
     4081 gaacgtgggt tcctttatcg gtgataatcc cgccccccat ccatgtctgg ccgttcaggg
     4141 cggacaccct gtaatcgccg gcaaactgca gatacccagc tccctgatta acggaattct
     4201 gcagaacgac ctcgccatta ttgccggtga acaggaggtt ttttcctgcg ttcagatcat
     4261 ttcctttctt tccatgcata tcccaagtcg tgtttccctg agtcagtgtg ccggtgccat
     4321 tagccgcatc atatttccac tgcagtactc cttcaccaga ggtgtaggct atagttttgt
     4381 caaaatcatt ttgcggttgc tgaccaagga aatcctgagt ggtgacgacc cagttatttc
     4441 catagttagt tcctgaggaa agcacgccaa tcagtaccca ttttttttgc agggaatcat
     4501 aagcaaacag gggggagccg ctgtccccca tattcagata attagccaga ggtccattcg
     4561 cgggattaaa aatgtcaccg gtctgagctg taatcatctg tccgttgtta taggagttca
     4621 gcgcgctgac ggttccccca acaagaaatc cgccattggt atacacttgg gtgcgattac
     4681 catttttgtc tttgacgtac tgcatccccc ccccaaggcg atagaattca gtaaagcgtc
     4741 cgccggcctg ataggcaccg ctcacagccc cgatgtcaga cacttctgcc ggcgccacct
     4801 ctgtcaccag tttactcagg cggcgggttt taatatcaag accgctatta ttatttgtac
     4861 caaccgcagt ataggtgttt ttggcgtatc cgaagctcat cgtgtcagaa ccgctgacat
     4921 gtttggcggt aaccacatac tgaggggagt aaagtgtatg gtctcccgga ggcaggctac
     4981 cggtagtaat agtggcacta ctgaaatcag ccatcggggc tttatcaagt ttaccgacca
     5041 gattgccctg cttgtcgtaa atggaaatat tcgtggtccc cggcgtaaac tgacctttat
     5101 tctcggcaaa gtcgcggaat atctgataag ggatctctgc actgaccgtt gctgacagag
     5161 ctggccatgc cgaggaaagt gataaagctg caacagacgt aagaataacc cgacgtgaaa
     5221 gtctgcgatg gcttttgcag gtaaccctgc gggccagttc ggagacagca atcaggcttt
     5281 tggttatatg gcaatactta agataatata ttttgttcat atagaaacat caacctaatt
     5341 ttgacaaaga ttaattacat aggatgtaga tggagcagtc cgagattcta ctaatatcaa
     5401 ttattaagca tctgtttttt agttggtgta atataatttt tattaaggta agcggaaaaa
     5461 caaccaaaaa aaacgcaaga tgttattttt ttatcatttt gagccagttt ggctgggggg
     5521 atactcttct cttaagcttc ttgctcaact ttaaaaatag ttatgccagt tcggtatcga
     5581 taaggggcag tttggatatc gagaaccacg gcagcctgac ggctgcaaat tgaaaactgc
     5641 ccctgttcgt cctggtgttg ccgcacgctc cgcagaatat gtatgctctg aggacctgtt
     5701 ttatttagga ctcatcctct tatgttgacc cgcaaaagta tagataccgt tctgctctct
     5761 gttggtgctg agaagctctc acaacgtgaa tgggactgga tgaagatgct caaacccatg
     5821 gacccaccac ctgcgatggt cgcggcctcc atcctggagc gccgtggcga cacagcagcc
     5881 cttacccggc tacaggata
//
LOCUS       DAZPCS010000193         5766 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZPCS010000193 DAZPCS010000000
VERSION     DAZPCS010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5766
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.196"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            144..1052
                     /locus_tag="ACHUQ7_003607"
     CDS             144..1052
                     /locus_tag="ACHUQ7_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HFR1136839.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIENRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1098..3089)
                     /gene="tkt"
                     /locus_tag="ACHUQ7_003608"
     CDS             complement(1098..3089)
                     /gene="tkt"
                     /locus_tag="ACHUQ7_003608"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFR1136840.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3367..4125
                     /gene="loiP"
                     /locus_tag="ACHUQ7_003609"
                     /gene_synonym="yggG"
     CDS             3367..4125
                     /gene="loiP"
                     /locus_tag="ACHUQ7_003609"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFR1136841.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLHQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(4331..5251)
                     /gene="speB"
                     /locus_tag="ACHUQ7_003610"
     CDS             complement(4331..5251)
                     /gene="speB"
                     /locus_tag="ACHUQ7_003610"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFR1136842.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5387..>5766)
                     /locus_tag="ACHUQ7_003611"
     CDS             complement(5387..>5766)
                     /locus_tag="ACHUQ7_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1136843.1"
                     /translation="GMNYQLYVRNYSRNTNYEIVATADGLDVLNGKQGSLNNNGYIVN
                     AGDSLVIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQNA
                     LPPCSGQAFPADNNGYAPPPCRK"
BASE COUNT         1387 a         1534 c         1518 g         1327 t
ORIGIN      
        1 atttattgaa tgttcgaaaa catacttttc taatgcaatg ttttttcgct ataccccttt
       61 atagattctg aattctcacc tgtcggctgt tttctcctca tctcatgtga catattccca
      121 tactttactc aaggaagatt gtcatgaaag cacctgtttc attaacctca tttcgcccac
      181 aaaaatctct tgctatcgcc attggcgtgc ttgccgtggt tgtgctgcca tttctctcgt
      241 attacaccgt taatgaaggt gaacgcggca ttttgctgcg ctacggaaaa atcgtaaaag
      301 ttgccgaacc ggggctgggt tttaagatcc cttttatgga aagcgtggag aaaatttcca
      361 cgcgtaatca ggctgtggtc tatcagggat tacaggccta cagccgcgac cagcaaccgg
      421 cacaaatgac agtatcagtc agcttccaca ttaaaccttc agaagcaggc gcggtttata
      481 ccacctacaa cactattgaa gcattaaaag atcgtctaat tgtccgtcag ttgccaactc
      541 agctggagaa cattttcggc caatataccg ccatcagcgc cgtacaggat cgtacaaaat
      601 tggttcagga tctgcaaaat gccatgcgca aagccgttgt tgggccagtg gtaattgacg
      661 gggtccagat tgaaaatatt gatttttctg acgcttatga aaagtcgatt gaaaaccgaa
      721 tgaaagcaga agtcgccatt gccacgcgta agcaaaatct ggaaacagag aaaatccagg
      781 cgcaaatagc ggtaactcag gcccaggcag aagcagacag taaactggcg gcagccaaag
      841 cagaagcgga aactattcgc gtaagaggcg cggcagaggc tgaaactatt cgtttaaaaa
      901 gtgcggctga agcagaagca atccgcctgc gcggtgaagc tctgcgggat aatcccggac
      961 ttgtagccct caccactgcc gaacgttggg acggtaaact gcccgatacc atgatccccg
     1021 gcagcacagt tccgtttatc tctactaagt aaaaaaaggg ccgctttcgc ggtccttctc
     1081 acaaaacgtc ttactgatta cagcagcgct tttgctttcg caacaacgtt atcaacagtg
     1141 aagccgaact cttcaaacag cagctctgcc ggagcagatt caccgaaggt ggtcatgccg
     1201 acgatagcgc cgttcaggcc aacatacttg taccagtagt cagcaatacc cgcttctacc
     1261 gcaacgcgtg cagtaaccgc tttcggcagt acggattcac ggtaagccgc atcctgcttg
     1321 tcaaatgcgt cggtagacgg catggacacc acgcgcgctt tcacgccttc ggcagtcagt
     1381 ttttcgtagg cagcaaccgc cagttcaact tcagaaccgg tggcgatgaa gatcagttcc
     1441 ggctgaccag cacagtcttt cagcacataa ccaccgcgcg cgatgtttgc cagctgctct
     1501 tcagtacgtt cctgctgcgc caggttctga cgggagagga taagcgcagt cgggccgtcc
     1561 tgacgctcaa cgccgtattt ccacgcgacc gcggattcaa cctggtcaca cggacgccat
     1621 gtagacatgt tcggggtcac gcgcagagaa gcgacctgct ctaccggctg gtgagtcggg
     1681 ccatcttcgc ccagaccgat ggagtcgtgg gtgtaaacca tcacctggcg ctgtttcatc
     1741 agcgcagcca tacgtacggc gttacgtgcg tattccacga acatcaggaa ggtggaggtg
     1801 tacggcagga agccaccgtg cagggagata ccgttagcaa tcgcggtcat accgaactcg
     1861 cgaacaccgt agtggatgta gttacctgca gcatcttcgt tgattgcttt agaaccagac
     1921 cacagggtca ggttagacgg tgccaggtca gcagagccgc cgaggaattc aggcaacagc
     1981 gggccgaacg cttcgatagc attctgcgac gctttacggc tggcgatttt cgccggatta
     2041 gcctgcagtt tagcgataaa ctctttcgct ttggcgtcga agtcagacgg catttcgcct
     2101 ttcatacggc gggtaaattc agccgcttcc tgcggataag ctttcgcgta agccgcaaac
     2161 ttctcattcc atgcagattc tttcgcctgg cctgcttctt tcgcatccca ctgagcatag
     2221 atttcagacg ggatttcgaa cggtgcatat ttccagccca gctgttcgcg ggtcagggca
     2281 atttcagcgt cgcccagcgg cgcaccgtgg gagtcgtgag taccggcttt gttcggggaa
     2341 ccgaaaccga tgatggtttt gcacatcagc agggacggtt tgtcagtcac tgcgcgcgct
     2401 tcttcaactg cgcgtttgat ggatgccgcg tcatgaccgt cgatgtcgcg aataacgtgc
     2461 cagccgtaag cttcgaaacg cattgctgtg tcgtcggtga accagccttc aacgtgacca
     2521 tcgatagaaa taccgttgtc atcgtagaac gcaatcagtt tacccagctt cagcgtaccc
     2581 gccagagagc aaacttcgtg ggagatgcct tccatcatgc agccgtcgcc catgaaggcg
     2641 taggtgtagt ggtcgacaat gtcgtggccc ggacggttaa actgcgccgc cagcgttttt
     2701 tctgcaatcg ccataccgac tgcgttggca ataccctgac ccagcggacc ggtggtggtt
     2761 tccacaccag cggtataacc tacttccgga tggcccggag ttttagagtg cagctgacgg
     2821 aagtttttca gttcttccat cggcagatcg taaccggtga ggtgcagcag gctgtagatc
     2881 agcatggagc cgtggccgtt ggacagcacg aagcggtcac ggtcagccca ggacggattc
     2941 tgcgggttgt gtttcaggaa atcacgccac aggacttcgg caatgtcagc catacccata
     3001 ggggcacccg ggtgaccgga tttggctttc tgtactgcgt ccatgctcag cgcacgaata
     3061 gcattggcaa gctctttacg tgaggacatt ttgactccag atcggatgat gaagggcacg
     3121 cccttaacga cttgacgaca gcgcgttttg ggctacgccg gaaaatttgc caacaattta
     3181 ccgcaagccg cgcgtcatgt acatggaaca tccttttgcc gcttcagaaa tctctggatc
     3241 atgctcgcat gttgcgcaat ctgctcgccc gtccgctgcg cttttcctta tactgagact
     3301 gagcgtcgat tcacctgcaa acggcgcatt tttagaataa tcctgacctt gtgcggaaga
     3361 gaaaacatga aaattcgcgc cttattggta gcaatgagcg tggcaacggt actgaccggt
     3421 tgccagaata tggactccaa cggactgctc tcatcaggag cggaagcttt tcaggcttac
     3481 agtttgagtg atgcgcaggt gaaaaccctg agcgatcagg catgtcagga gatggacagc
     3541 aaggcgacga ttgcgccagc caatagcgaa tacgctaaac gtctgacaac tattgccaat
     3601 gcgctaggca acaatatcaa cggtcagccg gtgaattaca aagtgtatat ggcgaaggat
     3661 gtgaacgcct ttgcaatggc taacggctgt atccgcgtct atagcgggct gatggatatg
     3721 atgacagata acgaagtcga agcggtgatc ggtcacgaga tggggcacgt ggcgttaggc
     3781 catgtgaaga aagggatgca ggtggcgctt ggtacaaatg ccgtgcgagt agctgcggcc
     3841 tctgcgggcg ggattgtcgg cagtttatct caatcacaac ttggtgatct gggcgagaaa
     3901 ttagtcaatt cgcaattctc ccagcgccag gaagcagaag ccgatgatta ttcttacgat
     3961 cttctgcacc aacgcggcat cagcccggca ggtcttgcca ccagctttga aaaactggcg
     4021 aagctggaag aaggtcgtca aagctcaatg tttgacgacc atcctgcatc cgccgaacgt
     4081 gcccagcata ttcgcgatcg catcagcgcg gatgggatta agtaaagcct agtggtgtcg
     4141 aacgtaggtc agataaggcg ttcacgccgc atctgacatt aatggcacat ttgttacctt
     4201 gtgcgcattg ccggatgcga tgctggcgca tcttatccgg cctacgggtg ccgaacgtag
     4261 gtcggataag gcgttcacgc cgcatccgac attaatggca cgttttaccc gtgcgcatcg
     4321 catctggtgc ttactcgccc tttttcgccg cctgaatata cagcatctcc agcgccagcg
     4381 tcgccgcagc cagagcggtg atttccgact gatcgtatgc cggagccact tccactacgt
     4441 ccatcccaac gatgttgaga tctttcaggc cgcgtaccag tttaatggcg cgatcggagg
     4501 tcaggccgcc aatcactggc gtaccggttc ccggcgcgaa agcaggatcc aggcagtcga
     4561 tatcaaaagt caggtaaacc ggcatatcac ccacaatctg tttcacctgg gcgataacgt
     4621 catccacgct gcgatcgttc acctggcagg cgtccagcac ggtaaagccg ttgtctttat
     4681 caaactcggt acgaatacca atctgcacgg aatgattcgg gtcgatcaga ccttctttcg
     4741 gcgcggtata gaacatagtg ccgtggtcaa attcacaacc gttcgcatag gtatcggtgt
     4801 gggcgtcaaa gtgtaccagc gccattttgc cgaaatgctt cgcatgagca cgcagcagcg
     4861 gcagcgtaac aaagtggtca ccaccgaagg agagcatacg cttaccggca gccagcagct
     4921 tctcggcgtg cgcctgcagc ttttcgctca tctcacgggc atcgccaaag gcatatacca
     4981 gatcgccgca gtccacgacg ttcagacgct cacgcatgtc gaaattccac gggaagcggt
     5041 tgtgttccca ggccagattc gtcgaaacct gacggatcgc tgccggaccg tggcgaccac
     5101 ccgcacgacc agaagtggcc atatcgaacg gcacgccagt aatcacccag tctgcatcgc
     5161 tgtcatacgg ctggaagttc atcggcaggc gtaaaaaacc aaaggcattg gaaaccagtg
     5221 agttatcgta ttgatgacct aaggtgctca tggatggact cctattacaa agtcgatata
     5281 aaaaaaccct tccgcgtcgt taggcccgac gaggaagggt tggatttgcg aaacacaagt
     5341 ttgtgctaat tatcgcctta aatgggcgag gcatcaagcg aggttgctat ttccgacacg
     5401 gtggcggcgc atagccattg ttatctgccg gaaaggcttg tccagagcac ggcggcagcg
     5461 cgttttgcgc cgggccttgt aattcaaagg cggcaaagcc gattaccccg gtattacgca
     5521 catcgccttg cgccgaattg gctgcgtagg aatcagcaac gttggcgaac tgaaaagccg
     5581 cttcagtatg tttatctttg cggaatcctt taatcactaa cgaatctccg gcattcacaa
     5641 tatagccgtt gttattcagc gaaccttgct tgccgttcag cacatccagg ccatcagccg
     5701 tagcaacgat ctcgtagttg gtgttacgac tgtaattacg gacatataat tgataattca
     5761 ttccga
//
LOCUS       DAZPCS010000194         5744 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZPCS010000194 DAZPCS010000000
VERSION     DAZPCS010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5744
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.197"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            1..173
                     /locus_tag="ACHUQ7_003612"
                     /pseudo
     CDS             1..173
                     /locus_tag="ACHUQ7_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(489..827)
                     /locus_tag="ACHUQ7_003613"
     CDS             complement(489..827)
                     /locus_tag="ACHUQ7_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1136844.1"
                     /translation="MVAETMFPNIAGSRSCIDCHHVAGRTFSKSARRIRGAPLLRRTS
                     RYDAQTSAFGISNDFRCISYVIPFWVVSSAMNVIPIPSLQTHYRSFITTTNGSAPVSR
                     YVPETGSHVP"
     gene            749..1876
                     /gene="ltrA"
                     /locus_tag="ACHUQ7_003614"
     CDS             749..1876
                     /gene="ltrA"
                     /locus_tag="ACHUQ7_003614"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFR1136845.1"
                     /translation="MFDQQRDGNLYKIWNRLCSGTWFPPPVLEKRIPKSNGKERILGI
                     PTVSDRIAQGAIKLFMEEKLDPIFHADSYGYRPGKSAHDALKQCAIRCWRYSWILEVD
                     ISAFFDHVRHDLVLKALEHHGMPKWVILYCRRWMEAPMQSCENGELITRTRGTPQGGV
                     ISPLLANLFLHYAFDLWMEREYRGVPFERYADDIVVHCSRMSDATRLKNRLSERFSEV
                     GLVLNAGKTNIAYIDTFKRRNVATSFTFLGYDFKVRTLKNFNGERYRKCMPGASNAAM
                     RKITETIKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGKFWSRNFNYRLWSAMQSRL
                     LKWMQSKYRLSNRKAQRKLTLVRKEYPKLFVHWYLLRASNE"
     gene            1959..2138
                     /locus_tag="ACHUQ7_003615"
                     /pseudo
     CDS             1959..2138
                     /locus_tag="ACHUQ7_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2165..2650)
                     /locus_tag="ACHUQ7_003616"
     CDS             complement(2165..2650)
                     /locus_tag="ACHUQ7_003616"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFR1136846.1"
                     /translation="MSEQKITGIDLAKTNFYLFSINAHGKPAGKTKLSRNQLLNWLVQ
                     QPKMTVAMEAGGASHYWAREIRKLDHDVILLPAQHVKAYQRCQKNDYNDAQAIAEACQ
                     HGTIRPVPIKTLEQQDVQTFLNMRRLVSMERTQLINHIRGLLAEYGIVFSKGAAELRQ
                     K"
     gene            complement(2906..3886)
                     /locus_tag="ACHUQ7_003617"
     CDS             complement(2906..3886)
                     /locus_tag="ACHUQ7_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HFR1136847.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(3886..5085)
                     /locus_tag="ACHUQ7_003618"
     CDS             complement(3886..5085)
                     /locus_tag="ACHUQ7_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR1136848.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(5259..5393)
                     /locus_tag="ACHUQ7_003619"
                     /pseudo
     CDS             complement(5259..5393)
                     /locus_tag="ACHUQ7_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5407..>5744)
                     /locus_tag="ACHUQ7_003620"
     CDS             complement(5407..>5744)
                     /locus_tag="ACHUQ7_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR1136849.1"
                     /translation="HWLWYAYNTKTGGVLAYTFGPRNDETCRELLALLTPFCIGMVTS
                     DDWGSYAREVPKEKHLTGKIFTQRIARNNRTLRTRIKRLARKTICFSRSVEIHEKVIG
                     SFIEKHMFY"
BASE COUNT         1574 a         1317 c         1368 g         1485 t
ORIGIN      
        1 ttgctgaact gctggaaccg ctgtatgaca tcctgcgcca gtatgtgctg atgcccggta
       61 aagtccatgc cgatgatatc cccgttccgg tccaggagcc gggcagcggt aaaacaagga
      121 ctgcccggct gtgggtctac gttcgtgatg accgtaacgc cggttcggaa attgcagcat
      181 aagttatcca tgctctgagg tgggtagagg tgggccctcc ggtcagggtt tacaggagca
      241 cagaccaaac gacccggtgc tgcttcattc taaggaatga ggtggtgagc gactcgggaa
      301 aaggcagaga tctactgtca cgtgggttcg ggaagctgaa cgaaagtaaa ctgtccgatg
      361 aagcatcgaa atcttagatg atgccaaaat ggggccgtac agccgtcccc tgataagcgc
      421 agaagacacc tgcttactga ctgcgcggca tccggtgtga aggcagcagg atcccgggcc
      481 agaccatctc agggaacgtg agaacctgtt tccggcacgt atcgggaaac aggggcggac
      541 ccattcgtag tagtgatgaa agatctgtaa tgggtctgga gcgaagggat ggggatcaca
      601 ttcattgcag aagacacaac ccaaaagggg atgacgtaac tgatgcaacg aaaatcattt
      661 gaaataccaa aagcactggt ctgggcatca tatctggatg tgcgtcgcaa caagggagcc
      721 ccccggatgc gacgggcaga ctttgaaaat gttcgaccag caacgtgatg gcaatctata
      781 caagatctgg aaccggctat gttcgggaac atggtttccg ccaccagtgc tggaaaaacg
      841 gatcccgaag tcgaatggca aggaacgcat tctggggatc ccaacagtat ccgatcgaat
      901 tgctcaggga gcgataaaac ttttcatgga agaaaagctt gacccgattt tccatgctga
      961 ttcatatggt taccgaccag gcaaatcagc tcatgacgct ctgaaacaat gtgccatccg
     1021 gtgctggcgt tatagctgga tactggaagt ggacatcagt gcgtttttcg accatgtgag
     1081 acatgatctg gtgctcaaag ctctggaaca ccacgggatg cctaaatggg tcatcctgta
     1141 ctgtcgacgc tggatggaag cgccaatgca gagttgtgaa aatggagaat taataacccg
     1201 aacacgaggc actccgcagg gcggggtcat tagtccgcta cttgctaact tgttccttca
     1261 ctatgcattt gatttgtgga tggaaagaga atatcggggg gtaccgtttg agaggtacgc
     1321 tgacgatatt gtagtacatt gttcacgaat gagtgatgca acaaggctaa agaacaggtt
     1381 atcggagcgc ttctcggaag tcgggctggt cctgaatgca gggaaaacga atatcgccta
     1441 cattgacacg tttaaaagga gaaacgtcgc aacgagtttc acctttctcg gatatgactt
     1501 caaagtgcgt acgctgaaga atttcaacgg cgaacggtac cgaaaatgca tgccgggtgc
     1561 gtcaaatgca gcaatgcgca aaataacaga aacaatcaag aagtggcgta tacatcgctc
     1621 aacagctgag agtttgctgg attttgcgag gcgctacaat gcgatagtga gaggctggat
     1681 cgagtactac ggaaagttct ggtccagaaa tttcaactat cgactgtgga gtgcaatgca
     1741 gtcacgtctg ctcaagtgga tgcagtctaa atacagactt tcgaaccgga aggctcagcg
     1801 aaagctgacg ctggtaagga aggagtatcc gaagctattt gttcactggt atttactacg
     1861 tgcatcaaat gagtgatcaa gagccgtatg acgcgagagt gtcacgtacg gttccgtgag
     1921 cagccgggga gtgaaattct ctccggctga ctcgactcgc gggttccgac agcgatgacg
     1981 aacatgcggc ggtgttgtac tcgctgatcg gcacatgccg tctgaacaat gtggaaccag
     2041 aaaaatggct gcgttacgtc attgagcata tccaggactg gccggcaaac cgggtacgtg
     2101 atctgttgcc ctggaaagtt gatctgagct ctcagtaaat atcaatacgg ttctgacgag
     2161 ccgcttactt ttggcgtaat tctgctgcgc ctttactgaa cacaatgccg tattcagcaa
     2221 gaagtccacg aatgtgattt atcagttgtg tcctttccat tgaaaccaac cgacgcatat
     2281 tcaaaaaagt ctggacgtcc tgttgctcca gagtttttat tggtacgggt cgtatagttc
     2341 catgttggca ggcctcagct attgcctgtg cgtcgttata atcatttttc tggcaccgct
     2401 gataagcttt aacatgttgt gccggaagca atattacatc atggtcgagt ttcctgattt
     2461 cccgggccca gtaatgagat gccccgccag cctccatcgc aacagtcatt ttaggctgtt
     2521 gcaccagcca gttaagcagt tgattgcgag aaagtttggt ctttcctgcg ggtttgccat
     2581 gagcattgat actgaaaaga taaaagttcg ttttagccag atcaatgccg gtgattttct
     2641 gttcagacat ggtatcccgc ttacttttgt tgttaaagtt tatccatcat ggcgcaattg
     2701 aaggctgccg tgaaagaggg ggaagactat ctcatcggtt actgttaaac ggctaagaga
     2761 aactatcttt ttcaagcaaa gaagatacgg tgaaacaggc gatttcaccg tgaaatactg
     2821 ttcaaaaaaa acgctaataa aagcaaaatt tcttttaaaa cagccaatta aaaactggcg
     2881 agttgctggc gaaaaacttg aattttcaca gcgaattcag attatctttt agtacaagag
     2941 caatcatacg atctaactta tcctgaactt ccaacggtaa tctgccgaat tcatatgaga
     3001 acgtacgtcc ctttactcgt tttcgagcaa atttatcttt actatcaaat ttccataaca
     3061 aagttacaac cgctttatcc tttactcctg aatcaagcaa tgaagacatt tccttggtta
     3121 taagccgcat aatactgttt ttaacctcgt cttcagaaag attgtcatca gcagtaataa
     3181 gctcaatact cggcgagata ttcttcacaa cttcatcaat agatatatca cccagcctca
     3241 gtcgttcact taattcagcc agttgcctgt aatcggcgta tgtcagctca gactgaacag
     3301 gaaaaagaga cacaaaatct ttaggtacgg acgctgcttg gagggcacga gtcaccttag
     3361 ctgccgacaa tccctctctt tccgcaattt gcttctggtt cataccggcc tctttgagac
     3421 gtactaaacg caacccaatt tcacgaatat tatgttcaag tgacgtctgc aaatctttcg
     3481 ccagatgctg agcttcagaa acagtaagct catcatccgt tacaagtaca cgtaatccaa
     3541 ccttgcatag caaagctgct gcccgacgac gggaaccatc caaaatctca ataagatcgc
     3601 cagtccgaat cccaatacat ggatagaatt gttgtaaacg tatagttctg gtgatgtctt
     3661 ttagtgagtc cggcgtcagg gctgtctgtt cgcggccatt cacctcttca accacaaaag
     3721 tttgagtatc cactttgtca tgatcaacct tgatctcagt aaattttgcc tttcggccag
     3781 tattgagggt aaatacatga actggagccg atatttcttc agtattattc aggatatttg
     3841 tattcagcgt acggccaata gtcggtcggt gctttctgtt ttccatcagt agttggccct
     3901 tataaattca atacgatcaa aaactgcttt agcgaaatct tctgctgcca tacgtgcatt
     3961 tttcaaagct tcaccactgc caacataagt aacaggattc gcagatatca cggtatcaaa
     4021 cgactcgccg cttctctcaa aaccatcaag acgtggcatt gaaacatcaa gcatgtcacc
     4081 accgaaaatc tcttttgtca ggctatggca gtatttatga tctgacttat tcgccagttt
     4141 cgacatgaag ccaatatttg cctgcaaacg gcatgaacat ccatcctgtt caataatctg
     4201 tacaagttca ggtaaacgag ccaaatattt aagcgtggaa tggaaatcaa cttgtgctgg
     4261 cggcaccggt gtaaacatga tatctgcggc agcaatggca tttttcaaaa atgcgtccag
     4321 atgcgggcct gtatcaataa ggatgaagtc gaaatcatgc ttcagcttat caataatatt
     4381 ctcacgcaat atcgcgtgtt tgttttgtcc taatagatgc tcctcgcaca gagtatccca
     4441 gtttgatgca ataaaggcgt cgtcaataga agccggcata acataaacgc cagggataac
     4501 tgacggaacg ataaaatctt ccagtaattc ctcacgactc acgttctgca acatagcctg
     4561 cggtgcggtt gtatcaacca gccccaccgc atgcaagtag ttcagaaaca tagtggctga
     4621 tgactgggga tctaaatcaa ggagcagaat acgtaaatct tctgacagca ggtgaggatg
     4681 agcacgcagg gcgtgagcta cggaaacact actcacggtt ttagacacac cgccttttag
     4741 actaccgatg aatatggaat atgcctcact ataacgatcg cgatatttag gaatgccacg
     4801 atgtgcatag atatcgataa tgttctggat ggttaatgca tattttgtcg cggttcctgc
     4861 tgggcgtttt tcaaactgat agccctgagc ctccatctca ccgactgcac catccacgct
     4921 tgcgcgcgtt aattttggaa gcttagctac agcagacttt gaatagactt gataaaaaac
     4981 atcagcctga aactcctgct tttgctcctg aatctgtttt gttagggcga gcaacatttt
     5041 atctgcacgc tgcgcaacct tacttaactg ctcaaaacta gtcattcccc ctccgtgcta
     5101 tgtaggattt taacttatta caaaaaaaac tgcattagca gggtaatgca tgcaagtaca
     5161 tattgtacat gcttatatta aaaatgcgcg cattgctgat ttattaatca tcggaatgaa
     5221 tattactgta agatagtatg cctttccggg agaaagacgc tgatttcccc cgaataatcc
     5281 agttcaccac cacactccgg acaacagctt tcttcaggct ccagatggtg tatttcgcgg
     5341 gaaacatgtt ccggtaacgg acggcgatga cgtgactgtc gcaacaggcg ggggtagtgc
     5401 ttccaatcag tagaacatgt gcttttcaat gaaggagccg atgacttttt cgtggatttc
     5461 cacggaacgc gagaagcaga ttgttttacg tgccagccgc ttgatgcggg tgcggagggt
     5521 ccggttgtta cgtgcaatac gttgcgtgaa gattttgccg gtcaggtgtt tctccttcgg
     5581 cacttctctg gcgtagctgc cccagtcatc gctggtcacc atgccgatac agaacggagt
     5641 caacagggct aacagttccc gacaggtctc atcattccgt ggcccgaaag tgtaagccaa
     5701 cacaccgcca gttttagtgt tgtacgcata ccagagccag tgtt
//
LOCUS       DAZPCS010000195         5740 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZPCS010000195 DAZPCS010000000
VERSION     DAZPCS010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5740
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.198"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..241
                     /locus_tag="ACHUQ7_003621"
     CDS             <1..241
                     /locus_tag="ACHUQ7_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1136850.1"
                     /translation="FGIFYLVTRVCGFANGYFRVRTSEPVRGNVFLHNMTAFNKLAPR
                     TVGKREHKRGMYVYYCIIFCHRVILNGPARGPCGC"
     gene            complement(238..1179)
                     /locus_tag="ACHUQ7_003622"
     CDS             complement(238..1179)
                     /locus_tag="ACHUQ7_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HFR1136851.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLVGYTWSRDIQLRE
                     DASIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGME
                     LGWTVIELQAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDGVPV
                     NNAAKTWATSTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQSLLTNLQTNTISYHQNGVPLNIRAVKWLKGRGVGNKDRIVAYTNDKKYVRYPLVPL
                     QSVPVQYRGLYQIVTYYGKLGAVEPVYKETISYVDGI"
     gene            complement(1191..1680)
                     /locus_tag="ACHUQ7_003623"
                     /pseudo
     CDS             complement(1191..1680)
                     /locus_tag="ACHUQ7_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1684..2904)
                     /locus_tag="ACHUQ7_003624"
     CDS             complement(1684..2904)
                     /locus_tag="ACHUQ7_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HFR1136852.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFVEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKVLAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            complement(2908..3651)
                     /locus_tag="ACHUQ7_003625"
     CDS             complement(2908..3651)
                     /locus_tag="ACHUQ7_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HFR1136853.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            complement(3542..5008)
                     /locus_tag="ACHUQ7_003626"
     CDS             complement(3542..5008)
                     /locus_tag="ACHUQ7_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HFR1136854.1"
                     /translation="MARNKQALRRTVQATADGYENFIARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEAVDAIPDDMTRKGIRITSEIDAKDRGILESQLDELQIWD
                     ALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRPETIGKGKFKGILPLDRWMVDPALTRR
                     IKDMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVE
                     RIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAYEALLKNIDLIRQFQ
                     SNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLFGQSPKGFSTGD
                     ADLANYYDRISSLQERRLRLPVRRILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRS
                     TVALNTTNAISTALGDGLMTLKAAMTDLRENSDVTGIGASITNEDIENAEDEAPPGIG
                     EPDDEPQEPSGGNPVSNQPTQDSEGGRRHRKWSLRWFK"
     gene            complement(5008..5740)
                     /locus_tag="ACHUQ7_003627"
                     /pseudo
     CDS             complement(5008..5740)
                     /locus_tag="ACHUQ7_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200750.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
BASE COUNT         1226 a         1618 c         1436 g         1460 t
ORIGIN      
        1 ctttggcatt ttttacctcg ttacgcgcgt ctgtggcttt gccaacggat acttccgcgt
       61 gcgcacgagt gaaccagtgc gtggcaacgt cttcctccac aacatgacgg cctttaacaa
      121 actcgcgccg agaaccgtcg ggaagcgtga gcacaaacgg ggtatgtacg tgtattactg
      181 catcattttt tgccatcggg tcatccttaa tggccccgcc agggggccat gtggctgtta
      241 aatgccatca acgtacgaaa tggtttcttt gtacactggc tcgactgcac ccagcttgcc
      301 gtagtaagtg acgatctgat acagaccgcg atactgcacc ggcacgctct gaagcggaac
      361 cagcgggtag cggacgtatt ttttatcgtt ggtgtacgca actatgcgat ccttattccc
      421 cacaccacgg cctttcagcc atttaaccgc gcggatattc agcggaacac cgttctggtg
      481 atagctgatg gtgttggtct gaaggttcgt caacagggac tggttacctg cagatgaaac
      541 gatgatgctg gacaacagag caaactgctc aggcgggatc agcaaatcac gcggaaccac
      601 agagtaacca gaagcggccc acgcatcaga cagcacctgg ttaatgcttg cgcggatttc
      661 gtccggtgtt gaggttgccc acgttttggc agcgttgttg acaggcacgc cgtccagggt
      721 aacaaggcct ttcaggttta atgcggaatc gccaacatat acctgttcat cgttatccat
      781 ctgccatttc agttgcatac cgtcatactt ctgcgtatca atcgggcggc cgacctgctg
      841 agcagcctgc aattctatga ccgtccagcc aagttccatc ccccacaggt tcagcgggtt
      901 accggatttg ccgatatcca cgttcacgcc agcaatagcg gttgagtctt tgcctaccca
      961 gttttttcca ttcggatttg cgccagtacc cgcagcggcg aagctggtat tcgtccagct
     1021 ggaaatgtca tctgcgatgg aggcgtcttc acgcagttga atatcgcggc tccaggtgta
     1081 ccccaccagt ggcaggttca gcgtctggtc gagtctctcc agctccccga tgagaaaggc
     1141 accagagctg tcaacggttg cctgatcaaa agtaatcatt cgtctgttcc ttaaatcttc
     1201 caggaaattt ctgcattgcc gtcagcatca ccggcacctg tgaattcagc gttggtcagc
     1261 accacgtttt tgccactgac tgacgtggac atgaatccac ccagaggcac tttgatggat
     1321 tcatcagtgg agatcgcctt ttttgatagt gctggcatca aaatcagaac cgagattaac
     1381 ggtcacgtag ccacgcttca tggcgtcgcc cgggaagttc ttgcctgttc ccacctggcg
     1441 aaccatgtcc ggctgcgacg tggtcggata agggcgcacg tagatcccct tcaccttgtc
     1501 tgcggtatca ccatctgcca gcggcacgaa aaaaccgtca tcatcgtatt taccagccag
     1561 gccataggca gcgaaggcgt tatcggattt aaggaccacc ggttcgacgg ttaagtcctg
     1621 cgggcgagag acagccccgg caatgccaac aggcatccgg tacagaaata cattattcat
     1681 tttttaccct ttacggtttg cccagaattc agcgttttgt ttgttcaggg aagcgatact
     1741 ggtcatgccc atgtttaggc gctgtgcatc gccggtggtg gcgcgggtgt ttcgcccttt
     1801 ggcaacctca gacacggcat taaacgccat gtcgaccgat tgtttcggta atttgcggat
     1861 atccgcatca ccgactatct ggcgaaccag cgttttgtca gcagaagcca gaacctcgcg
     1921 tttgaacgcg gtcggtttca tcttacggct cagatcgata cccggaacga taacttcggc
     1981 acgccaggct gagtcaccag taatcgtggt ttcctcttca tcgtcctcgc cgtcaccggt
     2041 cggattatcg tcaggcttat tatcgttatc gcccgtggca tttctttcca gcttagccag
     2101 cagggctttc agtaatgttt tgaggtcatc atcactgtcg ccggttggat ctccgcccat
     2161 ctctggtgct ttgtccggta gtggttgctg cggggacagg ttgatgttga gattaacgcc
     2221 ctgcggcaaa tccccctcat ctcctgtaac cgatgcggga gccgactcca ccagttcgtt
     2281 catggtgtcg gcatctcctg tcttgatggc cgcacgcatg cggttccacc agtttttctt
     2341 ttgatttgcc attgtgtctc tgtctccaat tgcacaacga tttccggctc tgcctttagg
     2401 gacaagagcc acatggtttc cggtaatatc gacctgctcg gctttacctg gctcggtctg
     2461 ctcgtactcc gcgtcatagc cgcacgacac ttcgcgcagg ccatcttcga taagctgaat
     2521 ggcgctttcg tctttgacga taaggtcagc cagcatcaaa tcagactgct cacccgtccc
     2581 gcgccggaca ttctggaggt gcccgacagc aagcactttc cagttctcgg gatttaccag
     2641 ccgcacattc ccgttttcat cttcaggatg caggatcgtg atgctcatcc cttcgaatga
     2701 ggcaagcgtg gccggatgga atacctgctc aggagaacgc gtgacgacta tttcaacgaa
     2761 cttatcgggt ttcagttttg gcaggtcatc agcaccatag agctgcttac ctgttcgtcc
     2821 tatcggcacg tctttgcaca gcaacgagcc gtcagccagg cggtatcggg tttccccgag
     2881 cctggtctca aaaaagtatt tcatgggtta tgctgccctt ttcattgcga atggaacgcg
     2941 aacaaacacg atctctttat agcagcggca gtttggcagc tcgcccgcgt ggccggtcat
     3001 gccgtccaaa gttggaggtg aatcccagcg gacgaattta cctttcatgt gatcgtggga
     3061 atctcggaca tcgccatcat cggctgtacg ccaaatataa ccatccgagc caatagctat
     3121 cgctcgggcc atatccagcg cgcccgttgc tcttcccagt tccgttcggg caatcagatc
     3181 ggccctagac ttagcaacat cgccggtaga tgcaatctct ttagcaaact gctccgcccg
     3241 accaccggtg acaacagcct ctatcgcttt gttctggatg tcgtacaccc tgtctgcggc
     3301 ctcgagaggc agagatttga tgtacttgac ctgttcggcg atgatggatt gcatcacctg
     3361 gcccacagga gcgctttcca caagattgcg gagctcgcga ctgatgttct tgctgtgttg
     3421 ccgccaaact ttctcgttct gccgggttag gtccgcagta aagttttccg cgacctttgt
     3481 cgcccagggg gtgatgattt cactgtaacg ctccagcgcc gcaataattt ccgtgatact
     3541 gtcatttgaa ccatcgtagc gaccatttac gatgtctccg accgccctcg ctatcctgcg
     3601 taggctggtt cgataccgga tttccgcctg acggctcctg cggttcgtca tcaggttcgc
     3661 cgatgccggg cggcgcttca tcttcggcat tctcgatgtc ctcgttggta atggatgccc
     3721 ctatgccggt tacgtcagaa ttttctcgca aatcagtcat agcggctttc agtgtcatca
     3781 gaccatcacc cagcgccgta ctgattgcgt tggtggtgtt taacgccacc gttgagcgat
     3841 cgacatcaga catttgccag agcgggttaa actcaaacgt gaaatcgtcc ggcagcggct
     3901 tgccaagttc cgaacgatgc atgatgtcca gtatccgccg caccggaaga cgtaaacgcc
     3961 tctcctgcaa cgaactgatg cgatcgtaat agttggcaag gtctgcatca ccggtagaaa
     4021 atcctttcgg ggactgtccg aacaaccgca ccagtgggat accaacagcg ccactaatct
     4081 gttctgcaaa ctgcgaaagg atgtcatcca gaccactgaa gctgtactga tgggtttcaa
     4141 acttatcccg cgagtccatg agcgtcatgc cttcattact ctggaactgt cgaatcaggt
     4201 cgatattctt cagcaacgct tcatacgcag gaccaccaag tgcgataagc tcgcgtagct
     4261 tctccacgct gtaggtacgc aaatgcgcct tgtagaccag ctgcgccgcg ccgacagtag
     4321 cgctgtcgaa cgcggtaaga cgatcccaga tacgctctac aaccgacatt ccccattcgt
     4381 tctcggtcat cttctgctga aatggcaacg tgacgccatc aaagcgaatc aggcgactgt
     4441 gatgaatgcg ccaggcagga attcccgttg ctgtggtcac cacatcgtaa aactcaggtt
     4501 tacccaggtc cggccccata tctttaatgc ggcgggtcag tgccgggtcg accatccagc
     4561 ggtcgagcgg gagaatcccc ttaaacttgc ccttaccgat ggtttcgggt cgcagcgggg
     4621 tcattggtgc ctgcccctca atcatgatga aacccaccgc gccgccgtag aggcgcgacc
     4681 atttcagcac gtcattcagc gcatcccaga tttgcaactc atccagttgt gattcgagaa
     4741 tgccacgatc ttttgcatca atttccgaag tgatgcgaat gcctttgcgg gtcatatcat
     4801 ccgggatagc atcgactgct tcgccgatga tccaggatga acgataggac cattccacca
     4861 gcatgcggtt acgactggtg aaattagccc ggtaggtgga tgctgagtgc tggttaggtg
     4921 tctgcatccc tactcgggca ataaaattct cataaccatc agctgtggcc tgcacagttc
     4981 gccgcagggc ttgtttgttt cgtgccatca ggcctgtctc cccagctgtt cccagatatc
     5041 cagcgatgta tcgattggcg cgaaggccat aatgaatgca tcagcaacgt ttggtgacgg
     5101 tatctcgcgt tttgcgaggt cttttttact ttcgaccatc acacgtccgt tacggtcgaa
     5161 atcacgatga ggtgttgtca gttccagttt cagcttttca agcaacggac aacgagaatc
     5221 tatgctgatc agctcatcca caggatactg ttctccgttg ttaatggcgt taaacgtatt
     5281 tctgaaacgg tcagccacca tgcctgagcc ttaagatttg cgaaaaagtc tttgttgggg
     5341 atgccgttgt actcgtcatc tggctcatgc acaccagcac cggcgttaaa cctctggtaa
     5401 ttcacacgtc gcgcgtatgc gttctcgctc ttccggtcag cgttaatttc agagaattta
     5461 gcaccggcag acgcaccaac accgatagag tcgtaaacaa tatctgcttc acgctccagc
     5521 gccgcctgat aagtacgctg gcagctcttc agtaattcat cttctttggc cttccattcg
     5581 tcggcccaga aaacaacgga tccgtgacgg taaacgttag cgcacttatc tgtaccactg
     5641 tcagccacgt caaagccaat acgctttctt ccactgggtt cgaaatttaa cgttttgtgc
     5701 gcatccactg cggcttctat ccaagacagt ttgatgattg
//
LOCUS       DAZPCS010000196         5702 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZPCS010000196 DAZPCS010000000
VERSION     DAZPCS010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5702
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.199"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..170)
                     /locus_tag="ACHUQ7_003628"
     CDS             complement(<1..170)
                     /locus_tag="ACHUQ7_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1136855.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSIG
                     EPFFFRVIVPFK"
     gene            246..2807
                     /gene="mutS"
                     /locus_tag="ACHUQ7_003629"
     CDS             246..2807
                     /gene="mutS"
                     /locus_tag="ACHUQ7_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFR1136856.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRSNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            2913..3565
                     /gene="pphB"
                     /locus_tag="ACHUQ7_003630"
                     /pseudo
     CDS             2913..3565
                     /gene="pphB"
                     /locus_tag="ACHUQ7_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(3616..4383)
                     /gene="ygbI"
                     /locus_tag="ACHUQ7_003631"
     CDS             complement(3616..4383)
                     /gene="ygbI"
                     /locus_tag="ACHUQ7_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HFR1136857.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRINVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVLPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LCSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            4579..5487
                     /gene="ltnD"
                     /locus_tag="ACHUQ7_003632"
                     /pseudo
     CDS             4579..5487
                     /gene="ltnD"
                     /locus_tag="ACHUQ7_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
     gene            5484..>5702
                     /locus_tag="ACHUQ7_003633"
     CDS             5484..>5702
                     /locus_tag="ACHUQ7_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HFR1136858.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQ"
BASE COUNT         1410 a         1469 c         1481 g         1342 t
ORIGIN      
        1 atcttaaagg gcactatgac tctaaagaag aagggttcgc caatcgatac aattttgcgt
       61 acttgcttca taagcatcac gcaaaagctg caaaacagca tctttcccgg aaccagcatc
      121 aagaactcgc cgttcgcttc ttcccctgaa atgattaact ccggtatcat gtgcgcctta
      181 tgtgattaca acgaaaataa aaaccatcac accccattta atatcaggga accggacata
      241 accccatgag tgcaatagaa aatttcgacg cccatacgcc catgatgcag cagtatctca
      301 agctgaaagc ccagcatccc gagatcctgc tgttttaccg gatgggtgat ttttatgaac
      361 tgttttatga cgacgcaaaa cgcgcgtcgc aactgctgga tatttcactg accaaacgcg
      421 gtgcttcggc gggagagccg atcccgatgg cggggattcc ctaccatgcg gtggaaaact
      481 atctcgccaa actggtgaat cagggagagt ccgttgccat ctgcgaacaa attggcgatc
      541 cggcgaccag caaaggtccg gttgagcgca aagttgtgcg tatcgttacg ccaggcacca
      601 tcagcgatga agccctgtta caggagcgtc aggacaacct gctggcggct atctggcagg
      661 acagcaaagg tttcggctac gcgacgctgg atatcagctc cgggcgtttt cgcctgagcg
      721 aaccggccga ccgcgaaacg atggcggcag agctgcaacg ctctaatccg gcggaactgc
      781 tgtatgcaga agatttcgcc gagatgtcgc tgattgaagg ccgtcgcggc ctgcgccgtc
      841 gcccgctgtg ggagtttgaa atcgacaccg cgcgccagca gttgaatctg caatttggca
      901 cccgcgatct ggtcggtttt ggcgtcgaga acgcgccgcg cggactttgt gctgccggtt
      961 gtctgttgca gtatgcgaaa gatacccaac gcacgaccct gccgcatatt cgttctatca
     1021 ccatggaacg tgagcaggac agcatcatta tggatgccgc gacacgtcgt aacctggaaa
     1081 ttactcagaa cctggcgggc ggtgcggaaa atacgctggc ttctgtgctc gactgcaccg
     1141 tcacgccgat gggtagtcgt atgctgaaac gctggctgca tatgccagtg cgcgataccc
     1201 gcgtgttgct tgagcgccag caaactattg gcgcattgca ggatttcacc gccgagttgc
     1261 agccggtact gcgtcaggtc ggcgacctgg aacgtattct ggcacgtctg gctttacgaa
     1321 ctgctcgccc acgcgatctg gcccgtatgc gccacgcttt ccagcaactg ccggagctgc
     1381 gtgcgcagtt agaaactgtc gatagtgcac cggtacaggc gctacgtgag aagatgggcg
     1441 agtttgccga gctgcgcgat ctgctggagc gagcaatcat cgacacaccg ccggtgctgg
     1501 tacgcgacgg tggtgttatc gcatcgggct ataacgaaga gctggatgag tggcgcgcgc
     1561 tggctgacgg cgcgaccgat tatctggagc gtctggaagt ccgcgagcgt gaacgtaccg
     1621 gcctggacac gctaaaagtt ggctttaatg cggtgcacgg ctactacatt caaatcagcc
     1681 gtgggcaaag ccatctggca cccatcaact acatgcgtcg ccagacgctg aaaaacgccg
     1741 agcgctacat cattccagag ctaaaagagt acgaagataa agttctcacc tcaaaaggca
     1801 aagcactggc actggaaaaa cagctttatg aagagctgtt cgacctgctg ttgccgcatc
     1861 tggaagcgtt gcaacagagc gcgagcgcgc tggcggaact cgacgtgctg gttaacctgg
     1921 cggaacgggc ctataccctg aactacacct gcccgacctt cattgataaa ccgggcattc
     1981 gcattaccga aggtcgccat ccggtagttg aacaagtact gaatgagcca tttatcgcta
     2041 acccgctgaa tctgtcgccg cagcgccgta tgttgattat taccggcccg aacatgggcg
     2101 gtaaaagtac ctatatgcgc cagaccgcac tgattgcgct gatggcctac atcggcagct
     2161 atgtaccggc acaaaaagtc gagattggac ctatcgatcg catctttacc cgcgtaggcg
     2221 cggcagatga cctggcttcc gggcgctcaa cctttatggt ggagatgact gaaaccgcca
     2281 atattttaca taacgccacc gaatacagtc tggtgttaat ggatgagatc gggcgtggaa
     2341 cgtccaccta cgatggtctg tcgctggcgt gggcgtgcgc agaaaatctg gcgaataaga
     2401 ttaaggcatt gacgctgttt gccacccact atttcgagct gacccagcta ccggagaaaa
     2461 tggaaggcgt cgccaacgta catctcgatg cgctggagca cggcgacact atcgccttta
     2521 tgcacagcgt gcaggacggc gcggcgagca aaagctacgg tctggcggtt gctgctctgg
     2581 cgggcgtacc gaaagaggtt attaagcgcg cacggcaaaa actgcgtgag ctggaaagca
     2641 tttcgccgaa cgccgccgcg acgcaagtgg atggtacgca gatgtctttg ctgtcagtgc
     2701 cagaagaaac ttcgcctgcg gtcgaagcac tggaaaatct cgacccggat tcactgactc
     2761 cgcgtcaggc gctggagtgg atttatcgct tgaagagtct ggtgtaataa caattcccga
     2821 tagtcttttg ctatcgggaa tattaacgac aactgacgaa tcaaataaaa ataccctgta
     2881 taataggaaa gcttatttta cagggtaaaa ccatgccatc tacacgctac caaaaaatca
     2941 atgcccatca ctatcgccat atatgggtcg ttggtgatat tcatggtgaa tatcagttat
     3001 tacaatcccg cttacatcaa ctctcttttt tccccgaaac cgacttactt atttctgtcg
     3061 gcgataatat tgatcgtggg ccggagagtc ttgacgtcct gcgcctgcta aaccagccct
     3121 ggtttatctc cgttaaaggc aaccacgaag caatggcgct ggatgcgttc gcgaccggcg
     3181 atggcaatat gtggcttgcc agcggtggtg actggttttt cgatttaaat gattgcaaca
     3241 agaggcaata gttctgttgc tgaaattcca tcaccttcca catattattg aaatcactaa
     3301 cgacataata aaatatgtca tcgcacatgc agattatccg ggtgatgaat atctctttgg
     3361 caaagaaata gcggagagcg aattactctg gcctgtttat cgtgtgcaga aatcgcttaa
     3421 tggcgagtta caacaaataa acggcgctga ttattttata tttggacata tgatgtttga
     3481 taacattcag acgttcgcta accagattta tattgatacc ggatcgccga aaagtgggcg
     3541 gctgtcattt tataaaataa ggtagtctca tgcttcttct gtgaagcatg agtaaccaga
     3601 tgttatttca ggccattatt catttttcgc taccagcaaa gagagatcct gcttcgccag
     3661 cgcgcgactg gcactctccg gcaaaccgtc gtctgtaata atctgatcaa actcgcttaa
     3721 tggtaacgcc agccatgtcg ccacctgacc atatttcgtc gcatcacaga ccaaaactcg
     3781 ctggcggctg gcactggcaa tcgcccgttt caccgtgact ttatcttccg ctggcgtaga
     3841 aatcccccgc acactccatg acgatgcaga aataaaagcc tgatcaatca tcaggctgca
     3901 cagcatggtc gcagcggctt ccccgacaca ggaacggttt tcccgacaca ctgcaccgcc
     3961 agtgtgaata attgtgcaat tactgttgtc gagcaagtag ttcgcaataa cgaaatcgtt
     4021 tgtgaccaca gtcagtgact ccatgtgaat cagatgctgt gctatcgcta acgtggtcgt
     4081 tcccgcatcc agatagatac aacttcccgg cagaacaaga cttgccgcca gcttgccaat
     4141 agccgctttt tgcgtcattg ccagcgcagt ttttacctga tgagaaggtt catgcgccac
     4201 gcgtcccgga gactggacgc ctccggacac cagcacaacg gctccctgct gctccagttt
     4261 ttgtaaatcc cgacgaatgg tcatatgtga cacattaatt ctgtccgtta gttcagcaat
     4321 actgacaatg cctttttcag ctaccatctc aaggatgatt tggcgacgct ctacgggtat
     4381 caacttttgc tccttccttt gtcctgctga cattctacgc tatttgcctg cgaaacgtgc
     4441 gcggcgcaac taacgcttag ttcacataaa ataacacaaa atgttaattt atgtgaatca
     4501 tctcaccata tcgttatctc ccagcgctta tgttcacaat atcaaacaaa atatcactta
     4561 aattaacaag gagagcagat gaaaacggga tctgagtttc atgtcggtat cgttggctta
     4621 gggtcaatgg gaatgggagc agcactgtca tgtgtccgcg caggtttttc tacctggggc
     4681 gcagacctga acagcaatgc ctgcgctacg ttgaaagagg caggtgcttg cggggtttct
     4741 gataacgccg cgacgtttgc cgaaaaactg gacgctctgc tggtgctggt ggtcaatgcg
     4801 gcctaggtta aacaggtgct gtttggtgaa acaggcgttg cacaacatct gaaacccggt
     4861 acggcagtaa tggtttcttc cactatcgct agtgctgatg cgcaagaaat tgctaccgct
     4921 ctggctggat tcgatctgga aatgctggat gcgccagttt ctggtggtgc agtaaaagcc
     4981 gctaacggtg aaatgactgt catggcctcc ggtagcgata ttgcctttga acgactggta
     5041 cccgtgctgg aagccgttgc cggaaaagtt tatcgcatag gtgcagaacc gggactaggt
     5101 tcgaccgtaa aaattattca ccagttgtta gcgggcgtac atattgctgc cggagccgaa
     5161 gcgatggcac ttgcagcccg tgcggggatc ccgctggatg tgatgtatga cgtcgtgacc
     5221 aatgccgccg gaaattcctg gatgttcgaa aaccggatgc gtcatgtggt ggatggcgat
     5281 tacaccccgc attcagccgt cgatattttt gttaaggatc ttggtctggt tgccgataca
     5341 gccaaagccc tgcacttccc gctgccattg gcctcaacag cattgaatat gttcaccagc
     5401 gccagtaacg cgggttacgg gaaagaagac gatagcgcag ttatcaagat tttctctggc
     5461 atcactctac cgggagcgaa atcatgatca agattggcgt tatcgccgat gattttaccg
     5521 gcgcgacgga tatcgccagt tttctggtgg aaaacggtct accaacggta caaattaacg
     5581 gtgttccaac aggtaaaatg ccggaagcaa tcgacgcact ggtgatcagc ctgaaaacgc
     5641 gctcctgtcc ggtggttgaa gccacacagc aatcgctggc ggctctgagc tggttgcaac
     5701 ag
//
LOCUS       DAZPCS010000197         5590 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZPCS010000197 DAZPCS010000000
VERSION     DAZPCS010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5590
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.200"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(1..279)
                     /locus_tag="ACHUQ7_003634"
     CDS             complement(1..279)
                     /locus_tag="ACHUQ7_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1136859.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDGYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
     gene            complement(447..602)
                     /gene="hokD"
                     /locus_tag="ACHUQ7_003635"
     CDS             complement(447..602)
                     /gene="hokD"
                     /locus_tag="ACHUQ7_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR1136860.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(1254..1670)
                     /locus_tag="ACHUQ7_003636"
     CDS             complement(1254..1670)
                     /locus_tag="ACHUQ7_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1136861.1"
                     /translation="MTELTKEQLIEEAKLKIAITKCHPNSGMARVEGELFKIARASLE
                     AEPIAWRYRYVKKGVMDSQGELWVGDWKYVPKKEDCNDRPNYEIQALFTAPPVPVTSE
                     ELVKAVHFYEQLKRENPPASGNQINGLTMSVKRPAN"
     gene            1697..1837
                     /locus_tag="ACHUQ7_003637"
                     /pseudo
     CDS             1697..1837
                     /locus_tag="ACHUQ7_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
     gene            1837..2887
                     /locus_tag="ACHUQ7_003638"
                     /pseudo
     CDS             1837..2887
                     /locus_tag="ACHUQ7_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(2915..3004)
                     /locus_tag="ACHUQ7_003639"
     tRNA            complement(2915..3004)
                     /locus_tag="ACHUQ7_003639"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2968..2970),aa:Ser,seq:cga)
     gene            3098..3895
                     /gene="mtfA"
                     /locus_tag="ACHUQ7_003640"
     CDS             3098..3895
                     /gene="mtfA"
                     /locus_tag="ACHUQ7_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFR1136862.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            3996..4071
                     /locus_tag="ACHUQ7_003641"
     tRNA            3996..4071
                     /locus_tag="ACHUQ7_003641"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4029..4031,aa:Asn,seq:gtt)
     gene            4233..5495
                     /locus_tag="ACHUQ7_003642"
                     /pseudo
     CDS             4233..5495
                     /locus_tag="ACHUQ7_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         1508 a         1326 c         1293 g         1463 t
ORIGIN      
        1 ttacctccag atccgttgct ggaatgtgca ggacggacgc ggtggccgtt cggaataagg
       61 gagcctgacg aagattatcc agtgacgata atcgagactg agggctttct taatctcgta
      121 tccgtgtctg cggtagcgct gaatcagcca ttcagcctgt tcttcggtgc aggggtcgtg
      181 ctgatacccg tcagatttga atgcatgaga acgccgcccg tgcctgctgg caggggcggc
      241 agagttatcc gaattgtaaa atttggtatc gtgcgccatc tgttttctct gctggcgcag
      301 caggtgccag ttgttcaggc tgacggatgg attgtaaacc agaacgacca gaaaaaacaa
      361 aacccgccga agcgggttaa gtgcgggtgc gttgaggatg cctgacacat cagcggtggc
      421 gagggatttc tcccccgccg ggtctcttac tcctcaggtt cgtaagctgt gaagacagcg
      481 acctccgtct ggccggttcg gattcgtacc tcgcagaggt ctttcctcgt taccagtgcc
      541 gtcactatga cggttaaaca gatgacgata agggcgatta acatcgcctt ttgctgcttc
      601 atagcctgct tctccttgcc tttcggcacg taagaggcta acctacatgt gttcagcatg
      661 gattgagcct cagattaatg ttaagcgtct tgcaggacgc gtaatgttaa ctggggcttt
      721 tctctatctg ccttttggtg ttcatgcctg agacagatag cctcaagcac ccgcagtcat
      781 tctacttaac taagatttcc ctgcaaaccg tttttgtccg gcacagtaaa tatccaacta
      841 aaccaatagc gttcgctgta tttaccgcca gtattcaatg cacatgaccg ccatgaacac
      901 ccctaaaaaa agggcattta tatgtccaaa cattaatatc aaaacatcaa ttttttccat
      961 ataccttgct gtgaagatga tgggcataca tgatgcgaac aaccagaacg caacaaacaa
     1021 aaactgcaat gcgtttttca ttattccccc tacaatcaat gtgcaataac atttaaacac
     1081 acctcaattt ggccggacat ataaatatct aaaccagaaa aaatcactta catagcgtta
     1141 caaactcttt agtctaaata ctcatcgtaa aacattcccc atacttatca gtccgttccg
     1201 cgccaggtag cttattgcct tatctggcaa cctataatca ggtttccgct ttttcagttg
     1261 gctggtcgtt taaccgacat agttaaccca ttaatctggt tgccggatgc tggtggattt
     1321 tcgcgtttta attgttcata aaagtgcaca gctttaacca gttcttctga tgtaaccggg
     1381 actggtgggg cagtgaataa ggcctgaatt tcatagttcg gcctgtcgtt acaatcctct
     1441 tttttcggta catatttcca gtcaccaacc cacaactccc cctgagagtc cataacacct
     1501 tttttcacgt agcgatatcg ccacgctatc ggctctgctt ccagcgatgc ccgtgcaatt
     1561 ttgaataact cgccctctac tcgcgccatc cctgaattgg ggtggcattt cgtaatcgct
     1621 atttttaatt tggcttcttc gattaattgt tcttttgtta attcagtcat tttcattacc
     1681 gccctggtaa tgctgccacc agcgaaaatg gttaatggaa aacgggcttc cgttctatac
     1741 caatcgtagt ggcaaaccga ttgtcagccg ggatctattt acctgcaata aaactttacc
     1801 accacgcgag gtagagccga attttggtgc gatctgatgg gaagacgaag gaaaaatcct
     1861 gaacacgaaa aattacctcc aaatgtatac ccaaataaat atagttatgt atggaaacca
     1921 acatccagag aatctgtaac actaaccgcc atcaaggatg gtttagctgc tttatggaaa
     1981 aagtatgagg aaactgtaaa taatcgcgat cgtgcaatga cattcggtcg cttgtgggaa
     2041 aaattcctcg ccagcgccta ttacagtgac ctcagtccaa gaacacaaaa agattatctg
     2101 caacatcaaa aaaagttgct tgccgtattc ggtaaggtgc cggcagattc cataaaacca
     2161 gaacacatcc gtcgatacat ggacaagaga ggggaacaga gtaaaacgca agccaaccat
     2221 gaaaaaagca gtatgtcccg cgtttacagt tgggggtatg agcgagggta cgtgaaggct
     2281 aacccatgtg caggtgtaag taaattcaag gccaaaaacc gcgaacgata tgtaaccgac
     2341 aaagaatacc aggcagtatt aagcgttgca cctcttcctg tttttatcgc aatggaaatt
     2401 gcctatctgt gtgcagcgag ggtttccgat gtgttatcgc tgaaatggga gcagattgga
     2461 aacgacggga tctttatcca gcaagggaaa acaggaaaaa aacagataaa agcatggagt
     2521 ccacgattac aggcggcgat cgaaaaagca aaacagttac caacatccgc ctatgtaatc
     2581 agcaatcaat acggcaaccg atatatgtac aaaggcttta acgaaatgtg ggtagaagca
     2641 agaaatcgcg caggcaaaat ttcaggtatt ttaaccgact tcacctttca tgatctgaag
     2701 gcgaaaggaa tttcagacta tgaaggaagc agtcgggata agcaactttt ctctggtcac
     2761 aaaaccgagg ggcaagtgct aatctatgac aggaaggtta aagtttcacc gacacttgat
     2821 gtcccgttac ctgaaaatat tccaagaaaa tattccaagt aattccaagt gtgatttttg
     2881 tcactgactt aatgatgtat aagtgattga attttggcgg agagaggggg atttgaaccc
     2941 ccggtggagt tgcccccact ccggttttcg agaccggtcc gttcagccgc tccggcatct
     3001 ctccgttcag atggttgcca tgatgccagg aaatttggca ttttaacagt ccctgtccgt
     3061 gcaattttgt tcaagtgacg agtttgcgag caaaacgatg attaagtggc cctggaaagt
     3121 acaagaatca gcacatcaaa ctgcccttcc ctggcaggaa gcactatcga tccccctttt
     3181 aacgtgtctg acagaacagg aacaaagcaa attggtcgct cttgccgaac gttttttaca
     3241 gcaaaaacgg cttgttcctt tacagggctt tgagctgaat tcattaagaa gctgccggat
     3301 agcacttcta ttttgcctgc ccgttctgga gttaggactg gaatggctgg atggttttca
     3361 tgaagtctta atttatcctg cgccatttgt ggtcgatgat gaatgggaag acgatatcgg
     3421 tctggtgcat aaccaacgta ttgttcagtc aggtcagagc tggcagcaag ggcctatcgt
     3481 tttgaactgg ttggatatac aagattcttt tgatgcttct ggttttaacc tgattattca
     3541 tgaagtcgct cataagctgg acacccgtaa cggcgatcgc gccagcggag ttccctttat
     3601 ttcgttgcgt gaggttgctg gctgggaaca cgatcttcat gctgcaatga acaacattca
     3661 ggaagaaatc gaattagttg gtgagaatgc ggcgagcatt gatgcttatg ctgccagtga
     3721 tcctgctgaa tgttttgccg tactttctga atatttcttt agcgccccag aactttttgc
     3781 tcctcgtttc ccttcattgt ggcaacgttt ctgtcaattt tatcaacaag atcctttgca
     3841 gagactgcat cacgctaatg atacagactc gttttcagcg acgaatgttc attaattaac
     3901 aactttgaag attaattaac caattgaaat ggcttatgaa atttagtgtt gacagacaag
     3961 gtaccgctaa gtaatatgcg ccccgttctc acgattcctc tgtagttcag tcggtagaac
     4021 ggcggactgt taatccgtat gtcactggtt cgagtccagt cagaggagcc aattttctgt
     4081 tttcatgcat ccttgcgaat ctttatctga tgttgattca acaggttagt gtgaaaactc
     4141 ttctcggtgc attttgattt taccctacgc atcgggaaaa attggtggtc aaatctggga
     4201 tcaggttagt tcgataatgg agtgaccccc atatatccct taccgacgca aaaatccgca
     4261 ccctcaagcc ttctgataaa ccctttaaag tctccgattc tcacggtctg tatctgctgg
     4321 tcaagccggg tggctcccgc cactggtatc tcaaataccg tattagcggt aaagaatccc
     4381 gcattgcgct gggtgcctat ccagccatct ccctgtctga tgcgcgacag caacgtgaag
     4441 gtatccgtaa aatgctggcg ctgaatatca acccggtaca gcagcgggct gctgaacgtg
     4501 gctcacgaac accggagaaa gtttttaaaa acatggcgct ggcgtgacat aaaagtaaca
     4561 ggaaatggtc gcagaacacc gccgaccgtc tgcttgccag cctgaacaat cacatctttc
     4621 cggtcatcgg gaacctacct gtatcagaac ttaaaccccg tcatttcatt gacctgctga
     4681 aagggatcga ggaaaaaggt ctgctggagg ttgcgtcccg cacacggcag cacctgagta
     4741 acataatgcg ccatgcggtc catcaggagt taatcgatac gaaccctgca gcaaaccttg
     4801 gcggcgtgac cacacctcct gtcagacggc actatcctgc cctgccgctg gagcggctgc
     4861 ctgaactgct tgaacgtatt ggggcatatc atcagggccg tgaactgacc cggcatgccg
     4921 ttctgctgat gctgcatgtg ttcattcgct ccagtgaact gcgtttcgcc cgctggtcag
     4981 agattgattt cacaaaccga gtctggacga tacccgcgac gcgagaaccc attattggcg
     5041 tgcgttattc cggccgcggg gcaaaaatgc gaatgccgca tatcgtcccc ctctcagaac
     5101 agtccatcgc cattctgaaa cagattaagg atatcaccgg taataatgaa ctgatcttcc
     5161 ccggcgacca taacccgtat aagccaatgt gtgaaaacac ggtcaataag gcactgcggg
     5221 tgatgggtta cgacacgaaa aaggatatct gcggtcacgg cttccgggca atggcatgca
     5281 gtgcgctgat ggaatcgggt ttatgggcaa aggacgcagt agaacgccag atgagtcatc
     5341 aggagcgcaa taccgtgcgc atggcttata ttcataaggc agagcacctg gaagcccgca
     5401 aagcgatgat gcagtggtgg tcggattatc tggaagcatg ccgagaatct tatgcaccgc
     5461 cttatacaat tggtaaaaat aagtttatcc catagtatac taacgaatcc cacactctgg
     5521 caaattacca ccacctctac aggaggtggt tctatataga cttttatcaa cttcatgacg
     5581 aaaaacacct
//
LOCUS       DAZPCS010000198         5560 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZPCS010000198 DAZPCS010000000
VERSION     DAZPCS010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5560
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.201"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..644
                     /locus_tag="ACHUQ7_003643"
     CDS             <1..644
                     /locus_tag="ACHUQ7_003643"
                     /EC_number="3.1.3.16"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0008138 - protein
                     tyrosine/serine/threonine phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HFR1136863.1"
                     /translation="VVGACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGCGGLGAIT
                     TGKDSQGKLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLRAFPRHIPAQN
                     AVLDVTFEFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCA
                     LGLSRSALVVAAWLLCYGHCKTVDEAIIFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(698..1303)
                     /gene="azoR"
                     /locus_tag="ACHUQ7_003644"
     CDS             complement(698..1303)
                     /gene="azoR"
                     /locus_tag="ACHUQ7_003644"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFR1136864.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWSEKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            1504..5406
                     /gene="hrpA"
                     /locus_tag="ACHUQ7_003645"
     CDS             1504..5406
                     /gene="hrpA"
                     /locus_tag="ACHUQ7_003645"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFR1136865.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLRYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVTIKLFDNPLEQKQAMWNGLRRLLLLNISSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
BASE COUNT         1378 a         1359 c         1540 g         1283 t
ORIGIN      
        1 atgtcgtagg cgcgtgcatt gtgttgatgg agctaatgat gatggttcag ttatggtggt
       61 cagtctggtt atgttggcca gtattatcgc tactcattat tggccgtggg tgcggtgggc
      121 ttggcgcgat aacaacaggg aaagatagtc aggggaaact cccgcccgcc gtttactggc
      181 tgacattgcc ctggcgcatc gggatgtggc tatctatgcg ttggttttgt cgtcgcctgg
      241 agccggtgag caaaatgact gctggtgttt atttaagggc gtttccacga catattccgg
      301 cacagaatgc ggttctggac gtcacctttg aattccctcg tggacgagcg acaaaagatc
      361 gactctattt ctgtgtaccg atgttggatc tggtggttcc ggaagagggg gagctccgac
      421 aggccgtggc gatgctggaa acattacgcg aagagcaagg cagcgttctg gtccattgcg
      481 cattgggatt atcgcgcagt gcgctggtag tggcggcatg gctgttatgt tacggacact
      541 gtaaaacagt tgatgaagcg attatcttta ttcgagccag acgctcgcat attgtgctta
      601 aggaagagca caaagcgatg ttgaaattat gggaaaacag gtaagtggat tgagatgtgg
      661 actgaatatc tacagtccac atcaagaccg tgtccggtta tgcagcaaca atgctgtcga
      721 tggctgcttt tgcgtcagac tgtgctttcg ctgccatttc cggaccgtat gcgatccctt
      781 cggcgaagac aaatttcaca tcggtaatgc cgataaagcc gaggaacgtg gacagatacg
      841 gcgtcaccag gtccgttggt ccatctttgt ggatcccacc gcggctggta ataacgatgg
      901 cttttttacc cgttaccaga ccttccggac cgttctcggt atagcggaaa gtaacgcctg
      961 cgcgtgcaac caggtcaaaa tagtttttca actgagttga gatgttgaag ttatacatcg
     1021 gtgccgcaat aacgataacg tcgtgggctt tcagctcggc aatcagctca tcggaaagcg
     1081 ccagagcttc ctgctgacgc ggagtcagcg gcgcatcgct cggacgcaga gcgccaacca
     1141 gttcgccatc cagtaccgga atcggatttg cagctaggtc gcgaacggtg atttcatcag
     1201 cggagtgctt ttcgctccat tgttcaacaa aataatcgga caactgatta gactgagagt
     1261 accctgccag gatgctggat ttaagaacta ataccttgct catggtgttt ccttatagat
     1321 gtttgaatgg acgatgcccc gttgcttgct gacactttat tcacaatcct gccacagaga
     1381 tcgcgcaata aatcgaagtc tatgttcgaa tttattgaac aacgcataga aagccgcgat
     1441 gtggtactct atatctatca tttaaaagaa aattaatcag gcagactact gcccactaac
     1501 gttatgacag aacaacaaaa attgaccttt actgccttgc aacagcggct ggattcgctg
     1561 atgctgcgcg acagactgcg tttttctcgc cgtctgcatt gtgttaagaa ggttaaaaat
     1621 cctgatgcac aacaggccat tttccaggag atggcgaaag agattgacca ggcggcaggg
     1681 aaagtcctgc tgcgtgaagc ggcacgaccg gaaatcactt atcctgacaa tttaccggtt
     1741 agtcagaaaa aacaggacat actcgaagcg attcgtgatc accaggtggt gatcgtcgcc
     1801 ggggaaaccg gctccggtaa aacgactcag ctaccgaaaa tctgtatgga gctggggcgc
     1861 ggtattaaag ggctgatcgg tcatacccag ccgcgtcgcc tggcggcacg aacggtggcc
     1921 aaccgtattg cggaagagct gaaaacggag ccgggcggtt gcatcggtta caaagtgcgt
     1981 ttcagcgatc acgtaagtga taacacgatg gtcaagctga tgaccgacgg gatcctgctg
     2041 gcggagatcc agcaagaccg cctgctgatg cagtacgaca ccatcattat tgacgaagcg
     2101 cacgaacgca gcctgaatat cgattttctg ctcggctatt tgaaagagtt gctgccgcgg
     2161 cgtcctgacc taaaaatcat tatcacttcc gcgactatcg acccggaacg cttttcgcgc
     2221 cactttaata atgcgccgat tattgaagtc tccggtcgga cctatccggt ggaagtgcgc
     2281 tatcgcccga ttgttgaaga agccgatgac accgagcgcg atcagttgca ggcgattttt
     2341 gacgccgtag acgaactaag ccaggaaagc cccggcgaca ttctgatctt tatgagcggc
     2401 gagcgggaaa ttcgcgatac cgccgatgcg ctgaacaagc tgaacttacg ccataccgaa
     2461 atcttgccgc tttatgcgcg gctttcgaac agcgaacaga accgcgtgtt ccagtcgcac
     2521 agcggacggc gcattgtgct ggcgaccaac gtcgcggaaa cctcgctgac cgtaccgggg
     2581 attaaatacg tcatcgaccc cggtacagcg cgtatcagcc gctacagcta tcgcaccaaa
     2641 gtgcagcgtt tgccgattga acccatttcg caggcgtctg ccaatcagcg taaaggccgc
     2701 tgtggtcgtg tgtccgaagg gatctgtatt cgtctctatt ccgaagacga tttcctctcg
     2761 cgcccggagt ttaccgatcc ggagattctg cgtaccaacc tggcctcggt tattttgcag
     2821 atgaccgcgc tggggctggg cgatatcgct gcgttcccgt ttgtcgaagc accggataaa
     2881 cgcaatatcc aggatggcgt gcgtctgctc gaagaactgg gcgcgatcac cacggatgaa
     2941 caggccagcg cctataaact gacgccgctc ggtcgccagc tctcgcagtt gcctgtcgac
     3001 ccacgtctgg cgcgtatggt gctggaagcg caaaaacatg gctgcgtgcg tgaggcgatg
     3061 attatcacgt ccgcgctctc catccaggat ccgcgcgaac gtccgatgga caaacagcag
     3121 gcatcggacg aaaaacatcg tcgcttccac gacaaagaat ccgacttcct cgcgtttgta
     3181 aatctgtgga attatcttgg cgagcagcaa aaggcgcttt cttccaacgc cttccgtcgc
     3241 ctgtgtcgta ccgattacct caactatctg cgcgtgcgcg aatggcagga tatctacacc
     3301 cagttgcgcc aggtggtgaa agaacttggc attccggtca acagcgaacc ggcggagtat
     3361 cgtgaaattc acatcgcctt actgaccggt ttgctttccc atatcggcat gaaagatgcc
     3421 gataaacagg aatataccgg tgcacgtaac gcgcgtttct ccatcttccc cggttctggt
     3481 ttattcaaaa agccgccgaa atgggtaatg gtggcggaac tggtagaaac cagccgcctg
     3541 tgggggcgca ttgctgcgcg tatcgacccg gaatgggtag aacccgttgc tcagcatttg
     3601 attaaacgca cctacagcga accgcactgg gaacgggcgc agggcgcggt gatggcaacg
     3661 gaaaaagtca ctgtttatgg tttgccgatt gttgctgcgc gcaaggtcaa ctacagccag
     3721 atcgattcgg cgttatgtcg tgaactcttt attcgccacg ctctggtgga aggtgactgg
     3781 cagacgcgtc acgcattctt ccgtgaaaac ctgaaactgc gggccgaagt ggaagagctg
     3841 gaacacaaat cacgtcgccg cgatattctg gttgatgacg aaacgctgtt tgagttctac
     3901 gaccagcgca tcagccacga tgtaatctcc gctcgtcact tcgatagctg gtggaaaaaa
     3961 gtcagccgcg aaacgcctga tttgctcaac tttgaaaaga gcatgttgat caaagaaggg
     4021 gcggaaaaaa tcagcaagct ggattacccg aacttctggc atcagggcaa tctcaagctg
     4081 cgtttgagat atcagtttga gccaggcgcg gacgcggacg gtgtgaccgt acatattccg
     4141 ctgccgttac ttaaccaggt tgaggaaagc gggtttgaat ggcagatccc cggtctgcgc
     4201 cgcgaattgg tgattgctct gattaaatcg ttgccgaaac cggtacgccg taattttgtg
     4261 cctgcaccaa actacgccga agcgttttta ggccgcgtca caccgctgga gttaccgttg
     4321 ctcgacagcc ttgagcgcga gttacggcgg atgaccggcg ttaccgttga ccgcgaagac
     4381 tggcactggg atcaggtgcc cgatcacctg aaaatcacct tccgcgtggt ggatgacaaa
     4441 aacaagaagc taaaagaagg gcgctcgcta caagatctga aagatgcgct gaaaggcaaa
     4501 gtgcaggaaa cgctgtccgc ggtggcggat gacggtatcg agcagagcgg cttacatatc
     4561 tggagttttg gtcagctgcc ggaaagttac gaacagaagc gtggcaacta taaagtgaag
     4621 gcgtggccgg cgctggtgga tgaacgcgac agcgtgacga tcaaactgtt tgataacccg
     4681 ctggagcaaa aacaggcaat gtggaatggt cttcgccgtc tactgctgct aaatatttcc
     4741 tcgccgatca agtatttaca tgagaagtta ccaaacaaag ccaagctggg gctgtacttt
     4801 aacccgtatg gcaaagtgct ggagctgatc gacgactgta tctcctgcgg tgtggatcaa
     4861 ctgatcgacg ccaatggtgg cccggtctgg acggaagaag gctttgctgc gctgcatgaa
     4921 aaagtgcgcg ccgaactgaa tgacacggtg gtggatattg cgaagcaggt cgagcaaatc
     4981 ctcactgcgg tgttcaatat caacaaacgt ctgaaagggc gggtggatat gaccatggca
     5041 ctggggcttt ctgatattaa agcgcagatg ggcgggttgg tatatcgcgg ttttgtcact
     5101 ggtaacggct tcaaacggct gggcgacacg ctgcgatatt tgcaggcgat tgaaaaacga
     5161 ctggaaaaac tggcggttga tccacatcgc gatcgtgcac agatgctgaa agtcgaaaac
     5221 gtccagcagg cgtggcagca atggatcaac aaactgccgc ctgcacgtcg tgaggatgaa
     5281 gacgtgaaag agatccgttg gatgatagaa gagctgcgcg ttagttactt cgctcaacaa
     5341 cttggtacgc cttatccgat ttcagataag cgtattttgc aggcgatgga gcagattagc
     5401 ggttaaccct gctatttgcc taataaatct aaaacccggt aagcatttag cgccgggttt
     5461 tttattaatt ctaaaacggt aaggacaaaa attcaggaat tcagaaaaat acaattctca
     5521 gctgcaagat gaataatgtt tatctacagc atttccttaa
//
LOCUS       DAZPCS010000199         5499 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZPCS010000199 DAZPCS010000000
VERSION     DAZPCS010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5499
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.202"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(89..625)
                     /gene="ssb1"
                     /locus_tag="ACHUQ7_003646"
     CDS             complement(89..625)
                     /gene="ssb1"
                     /locus_tag="ACHUQ7_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFR1136866.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            879..3701
                     /gene="uvrA"
                     /locus_tag="ACHUQ7_003647"
     CDS             879..3701
                     /gene="uvrA"
                     /locus_tag="ACHUQ7_003647"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFR1136867.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3736..4092)
                     /locus_tag="ACHUQ7_003648"
     CDS             complement(3736..4092)
                     /locus_tag="ACHUQ7_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFR1136868.1"
                     /translation="MTISELLQYCMAKPSAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4096..4512)
                     /locus_tag="ACHUQ7_003649"
     CDS             complement(4096..4512)
                     /locus_tag="ACHUQ7_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFR1136869.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="ACHUQ7_003650"
     CDS             complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="ACHUQ7_003650"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFR1136870.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
BASE COUNT         1290 a         1456 c         1443 g         1310 t
ORIGIN      
        1 cctccataac ggaggcttct tttttatggc tggcagatgc tttaatcatc caccttaaaa
       61 caatataacc tattgtttta atgacaaatc agaacggaat gtcatcatca aagtccatcg
      121 gcggctcgtt agacggcgct gccggagcgg actgctgcgg gcgagactgc gcgccgccgc
      181 tgaactgatt gccaccctgc ggctgctgag gctgacccca accgccctgc ggctgaccac
      241 caccgatatt gccacctgcc ggagcgccac caccctgacg accacccagc atctgcatgg
      301 tgccgccaac gttcaccacg acttctgtgg tgtagcgatc ctgaccggat tgatcggtcc
      361 atttacgggt acgcagctga ccttcgatat aaacctgaga acctttacgc agatattcgc
      421 tcgccacttc tgccagtttg ccgaacagca caacgcggtg ccattcagtc tgttctttca
      481 tctcgccggt cgctttatca cgccaggatt cggaagtagc cagcgtaatg ttggcaactg
      541 cgccaccatt tggcatgtag cgtacttccg ggtcctgacc cagattacca acgagaataa
      601 ccttgtttac gcctctgctg gccatgttcg tgtctcctga aaaaaatcgt tctgaataag
      661 tgtaaacgcg cgattgtacc attaccaata gcgcttttac tatgttgtga cctcggttcc
      721 gggaaacaaa cctggccaga cattgttaca caacactccg gataatgcat tccaatactg
      781 tatattcatt caggtcaatt tgtgtcataa ttaaccgttt gtgatcgccg gtagcaccat
      841 gccaccgggc aaaaaagcgt ttaatccggg aaaggtgaat ggataagatc gaagttcggg
      901 gcgcccgcac ccataatctc aaaaacatca acctcgttat cccccgcgac aagctcattg
      961 tcgtgaccgg gctttcgggt tctggcaaat cctcgctcgc tttcgacacc ttatatgccg
     1021 aagggcagcg ccgttacgtt gaatcccttt ccgcctacgc gcggcagttt ctgtcactga
     1081 tggaaaagcc ggacgtcgat catattgagg ggctttctcc tgccatctca attgagcaga
     1141 aatcgacgtc tcataacccg cgttctacgg tggggacaat caccgaaatc cacgactatt
     1201 tgcgtttgtt gttcgcccgc gtcggcgaac cgcgctgtcc ggaccacgac gtcccgctgg
     1261 cggcgcaaac cgtcagccag atggtggata acgtgctgtc gcagccggaa ggcaagcgtc
     1321 tgatgctgct cgcgccaatc attaaagagc gcaaaggcga acacaccaaa acgctggaga
     1381 acctggcaag ccagggttac atccgtgctc gtattgatgg cgaagtctgc gatctttccg
     1441 atccgccgaa actggaactg caaaagaaac ataccattga agtggtggtt gatcgcttca
     1501 aggtgcgtga cgatcttacc caacgtcttg cggagtcgtt tgaaaccgcg ctggaacttt
     1561 ccggtggtac agcggtagtg gcggatatgg acgacccgaa agcggaagag ctgctgttct
     1621 ccgccaactt cgcctgccca atttgcggct acagtatgcg tgaactggag ccgcgactgt
     1681 tttcgtttaa caacccggca ggtgcctgcc cgacctgtga cggccttggc gtacagcaat
     1741 atttcgatcc tgaccgcgtg atccaaaacc ccgagctgtc actggctggc ggtgcgatcc
     1801 gtggctggga tcgccgcaac ttctattact tccagatgct gaaatcgctg gcagatcact
     1861 ataagttcga cgtcgaagcg ccgtggggca gcctgagcgc gaacgtgcat aaagtggtgt
     1921 tgtacggttc tggcaaagaa aacattgaat tcaaatacat gaacgatcgt ggcgatacct
     1981 ccatccgtcg tcatccgttc gaaggcgtgc tgcacaatat ggagcgccgt tataaagaga
     2041 cagaatccag tgcggtacgt gaagaattag ccaagtttat cagcaatcgc ccatgcgcca
     2101 gctgcgaagg gacgcgtctg cgtcgggaag cgcgccacgt ttatgtcgag aatacgccgc
     2161 tgcctgctat ctccgacatg agcatcggtc atgcgatgga attcttcaac aatctcaaac
     2221 tcgctggtca gcgggcgaag attgcggaaa aaattcttaa agagatcggc gatcgcctga
     2281 aattcctcgt aaacgtcggc ctgaattacc tgacactttc ccgctcggca gaaacacttt
     2341 ccggcggtga agcccagcgt atccgtctgg cgagccagat tggtgcgggc ctggttggcg
     2401 ttatgtacgt gctggacgag ccgtctatcg gcctgcacca gcgcgataac gagcgcctgt
     2461 tgggtacgct tatccatctg cgcgatctcg gtaataccgt gattgtggtg gagcatgacg
     2521 aagacgcaat tcgcgccgct gaccatgtga tcgatatcgg tcctggtgcg ggtgttcacg
     2581 gcggtgaagt ggtcgcagaa ggtccgctgg aagcgattat ggcggtgcct gaatcgttga
     2641 ccgggcagta catgagcggt aaacgcaaga ttgaagtgcc gaagaaacgc gttccggcga
     2701 atccagaaaa agtgctgaag ctgacaggcg cacgcggcaa caacctgaag gacgtgacgc
     2761 tcacgctgcc agtcggtctg tttacctgca tcacaggggt ttcaggttcc ggtaaatcga
     2821 cgctgattaa cgacacactg ttcccgattg cccaacgcca gttgaatggt gcgaccatcg
     2881 ccgaaccggc accgtatcgc gatattcagg gactggagca tttcgataaa gtgatcgata
     2941 tcgaccaaag cccaattggt cgtactccac gttctaaccc ggcgacctat accggcgtgt
     3001 ttacgcctgt gcgcgaactg tttgcgggcg taccggaatc ccgtgcgcgt ggttatacgc
     3061 caggacgttt cagctttaac gtccgtggcg ggcgctgcga agcctgtcag ggcgacggtg
     3121 tgatcaaagt ggagatgcac ttcctgccgg atatctacgt gccgtgcgat cagtgcaaag
     3181 gtaaacgcta taaccgtgaa acgctggaaa ttaagtacaa aggcaaaacc atccacgaag
     3241 tgctggatat gaccatcgaa gaggcgcgtg agttctttga tgcggtgcca gctctggcgc
     3301 gtaagctgca aacgttgatg gacgttggtc tgacgtacat tcgcctgggg cagtccgcaa
     3361 ccacactttc tggtggtgaa gcccagcgcg tgaagctggc gcgtgagctg tcaaaacgcg
     3421 gcaccgggca gacgctgtat attctcgacg agccgaccac cggtctgcac ttcgccgata
     3481 ttcagcaact cctcgacgtg ctgcataaac tgcgcgatca gggcaatacc attgtggtga
     3541 ttgagcacaa tctcgacgtg atcaaaaccg ctgactggat tgtcgacctg ggaccagaag
     3601 gcggcagtgg cggcggcgaa atcctcgtct ccggtacgcc agaaaccgtc gcggagtgcg
     3661 aagcttcgca tacggcacgc ttcctcaagc cgatgctgta atcgttaagg ccgctttctg
     3721 agcggccttt tcctttcaga gttgtaccag caatttacgc ttttcttccg gtagtaaatt
     3781 cactgcctgc tgataagacg catccaccag ataatagatt tgcgaatccg gcagcgaacc
     3841 gtcgagatac acggtgctcc agtgcgcttt attcagatgg cggctaggac gcacatcgct
     3901 gtgctgctga cgtagcagct ccgccagctc cgggctggtt ttcagcgaaa cagctgggcg
     3961 attttctact tctttcacca tcgcaaacag tacatcttcc actttgatct gcgtcgcttt
     4021 ccagtcgtta tgcacgctct gttctgcgct tggttttgcc atgcaatatt gtagcaactc
     4081 cgaaatggtc attttttact ccccttgtag tgtcgcgatg atgcgacgcg atccgccgtg
     4141 gatgcgatgt tcccccagcc agatgccttg ccaagtgccg gtctgaatac gccctttatg
     4201 taccggcaat acaagcgatg ttcccagcat tgaggatttg atatgagaag gcatatcgtc
     4261 tgctccctca tagtcatgct cataatttcc gttgtcggga acggtgcgga ggaaaaaaca
     4321 ctccatgtcg tggcgtacgg tgggatcgca gttctcatta agtgtcagag aggcggaggt
     4381 atgttgcagc aacagatgca gtaagccgat gttaacgcgc ggcatatcag ccagctgatt
     4441 cagaatttca tccgttacca gatgaaaccc acgagatttg gcgctaagcg tgagcgtctt
     4501 ttgataccac atgtgctgct ccttgataaa actctcttaa tcagtttgca gcaagacagc
     4561 gaaaggataa aggtgtgatt aaaaaaacag cattcgggag agcatcactc tctcccgctc
     4621 tgtcagtatt ctgaattgac gatcacctct tcaccaaacg cacccgcttg tggcaagggt
     4681 ttgtaggtag agttggaggc gcgcagaatg cggataccac gagcgccgac atcgcgtgcg
     4741 gcggtaatat cgttatcgga atcgccataa aaaattcgga tatttttatc ctgcagccat
     4801 tgcgattttg tattttgccc tggtttatcg cccgcaaaga ttaccggatt catgttggtg
     4861 gcaggaatat gaaaattatc cgccagcgtt tttgaaaccg tttctgtttt cgtcgggcta
     4921 cgaccagtca caaagaagat cgcgtcaccg cggcgtacat gcatatcaat cagctggcga
     4981 gcgacctctt ttggaatgct gaattcatcc cagccattat tcattttttc ccagaacaca
     5041 ggatttttca gataatcttc gctttctggc gagaaggttt ttttgccgcg ccagaacccc
     5101 ggactggaaa aaagtaccgt gtcatcgata tcaaacccca ccgccattgg cggacgccct
     5161 gcgaggctat tttcaatttg tgcgaccgaa acccaatgaa ttggtgcctg ttcagcaagc
     5221 ctggcaacgt tagtcccagg gttaagcggt gaaggagatg aggccagggc aacagcggaa
     5281 ctgtttagag cgaacaataa gcaaacggca ctgattgcct gtgtgatctt gcgcatattt
     5341 ttccctaaat agtcagtatg ttgaaacttt ttattgtgag atttgttgca aaaaccatct
     5401 gaccataacg ccagctgccg gggaaaggaa ggattatctg cggtttttgt gatgaaaagc
     5461 aggatgatta tatttaaccc agtaaatacg ttcctgtct
//
LOCUS       DAZPCS010000200         5499 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZPCS010000200 DAZPCS010000000
VERSION     DAZPCS010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5499
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.203"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            539..1528
                     /gene="araF"
                     /locus_tag="ACHUQ7_003651"
                     /pseudo
     CDS             539..1528
                     /gene="araF"
                     /locus_tag="ACHUQ7_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
     gene            1598..3112
                     /gene="araG"
                     /locus_tag="ACHUQ7_003652"
     CDS             1598..3112
                     /gene="araG"
                     /locus_tag="ACHUQ7_003652"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFR1136871.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLAIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIGNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKALGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEARKAEGIIPVHSVRDNINISARRKHVLGGCVINNSWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            3127..4113
                     /gene="araH"
                     /locus_tag="ACHUQ7_003653"
     CDS             3127..4113
                     /gene="araH"
                     /locus_tag="ACHUQ7_003653"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFR1136872.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            4280..5080
                     /gene="otsB"
                     /locus_tag="ACHUQ7_003654"
     CDS             4280..5080
                     /gene="otsB"
                     /locus_tag="ACHUQ7_003654"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFR1136873.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITAALQQKRENNRSDDYESFSRSI"
     gene            5055..5499
                     /locus_tag="ACHUQ7_003655"
                     /pseudo
     CDS             5055..5499
                     /locus_tag="ACHUQ7_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310631.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
BASE COUNT         1359 a         1269 c         1471 g         1400 t
ORIGIN      
        1 gatattaatc gatgaatttg aattcatgat gaagtgatga ttttataaaa cgttttttca
       61 tttttgcgag atggctctca ttatacgtgt tctgttaatt aatgagcaca gtgataatta
      121 atggcgaaat gaatgacgcg cattttccac atctttgagt tgtggttatt acaccatttc
      181 aaaaactcaa cgccaggtaa tgcggcctat tgactggtta aaaagaagac atcccgcatg
      241 ggtaccgaag acaacaagga tttccaggct aatcttatgg attaatctgc tgtgcattca
      301 acaatttgtc tgacaaattg gcttttcctt atgtcttttc ccgctaaatt tatgcacgtt
      361 ctcactgtaa ttctgcgatg tgatattgct ctcctatgga gaattaattt ctcgctaaaa
      421 ctatgtcaac acggtcactt atcttttaga taaaaggtaa tgctttgttt tccgattaat
      481 ttaacgaatg tcattcgttt ttgccctaca caaaacgaca ctaaagctgg agagaaccat
      541 gcacaaattt actaaagccc tggcagccat tggtctggca gccgttatgt cacaatccgc
      601 tatggcggag aacctgaagc tcggttttct ggtgaagcaa ccggaagagc cgtggttcca
      661 gaccgaatgg aagtttgccg ataaagccgg gaaggattta gggtttgagg ttattaagat
      721 tgccgtgccg gatggcgaaa aaacattgaa cgcgatcgac agcctggcag ccagtggcgc
      781 aaaaggtttc gttatttgta ctccggaccc gaaactcggc tctgccatcg tcgcgaaagc
      841 gcgtggctac gatatgaaag tcattgcagt ggatgaccag tttgttaacg ccaaaggtaa
      901 gccaatggat accgttcctc tggtgatgat ggcggcgact aaaattggcg aacgtcaggg
      961 ctaggaactg tataaagaga tgcagaaacg tggctgggat gtcaaagaaa gcgcagtgat
     1021 ggcgattacc gccaacgaac tggataccgc ccgccgccgt acaacgggtt ctatggacgc
     1081 actgaaagct gcaggattcc cggaaaaaca aatttatcag gtacctacca aatctaacga
     1141 catcccgggg gcatttgacg ctgccaactc aatgctggtt caacatccgg aagttaaaca
     1201 ttggctgatc gtcggtatga acgacagcac cgtacttggc ggcgtacgtg caaccgaagg
     1261 tcagggcttt aaagcagccg atatcatcgg tattggtatt aacggtgtgg atgcagtaag
     1321 cgaactgtct aaagcacagg caaccggctt ctacggttcc ctgctgccaa gcccggacgt
     1381 acatggctat aaatccagcg aaatgcttta caactgggta gcaaaagacg ttgaaacgcc
     1441 aaaatttacc gaagttacag acgtggtact gatcacgcgt gacaacttta aagaagaact
     1501 ggagaaaaaa ggtttaggcg gtaagtaatt tgccggaaaa attcccctct gcatgatgca
     1561 gagggggtgt gaacgaccag tgattcacgg agacgttatg caacagtcta ccccgtatct
     1621 ctcatttcgc ggcatcggta aaacgtttcc cggcgttaag gcgctgacgg atattagttt
     1681 tgactgctat gccggtcagg ttcatgcgtt gatgggtgaa aatggcgcag gaaaatcaac
     1741 tctcttaaaa atcctcagcg gcaactatgc gccaaccacg ggttctgtag tgattaatgg
     1801 gcaggaaatg tccttttccg acacgaccgc agcacttaac gcgggtgtgg cgattattta
     1861 ccaggaactg catctcgtgc cggaaatgac cgtcgcggaa aacatctatc tcggccagct
     1921 gccgcataaa ggcggcattg tgaatcgctc attgctgaat tatgaggcgg gtttacaact
     1981 taaacatctt ggtatggata ttgacccgga cacgccgctg aaatatctcg ccattggtca
     2041 gtggcagatg gttgaaatcg ccaaagcgct ggcgcgtaac gctaaaatta tcgcctttga
     2101 tgagccaacc agctcccttt ctgcccgcga aatcggcaat cttttccgcg ttattcgtga
     2161 actgcgaaaa gaggggcggg tgatcttata cgtttctcac cgtatggaag aaatatttgc
     2221 cctcagcgat gccatcactg tctttaaaga tggacgttat gtcaaaacct ttaccgatat
     2281 gcagcaggtt gaccacgacg cgctggtgca ggcgatggtc gggcgcgaca ttggcgatat
     2341 ctacggctgg caacctcgta gttatggcga ggagcgcctg cgtcttgatg ctgtgaaagc
     2401 actaggcgtg cgtacgccaa taagtctggc ggttcgcagt ggtgaaattg ttgggctgtt
     2461 tggtctggta ggggcggggc gtagcgaatt aatgaaaggc ttgtttggcg ggacgcaaat
     2521 caccgccggt caggtttata tcgaccaaca gccgatcgat attcgtaaac cgagccacgc
     2581 cattgccgca ggcatgatgc tctgcccgga agctcgcaaa gcggaaggca ttattcccgt
     2641 gcactccgtt cgcgacaata tcaacatcag tgccagacgc aaacatgtgc tcggcggttg
     2701 tgtaatcaac aacagttggg aagaaaacaa tgccgatcag cacattcgtt cgctcaacat
     2761 caaaacgccg ggcgcggagc aactgatcat gaatctctca ggcggaaatc agcaaaaagc
     2821 cattctgggc cgctggttat cggaagagat gaaggtcatt ttgctggatg aacctacgcg
     2881 cggcattgat gttggcgcta agcacgaaat atataacgta atttatgcgc tggcggcgca
     2941 gggcgtggcg gtgctgtttg cctccagcga cttacctgaa gtcctcggcg ttgccgaccg
     3001 gattgtggtg atgcgggaag gcgaaatcgc cggtgaattg ttacacgagc aggcagatga
     3061 gcgtcaggca ctgagccttg cgatgcctaa agtcagccag gcagttgcct gagtaaggag
     3121 agtatgatgt cttctgtttc tacatcgggg tctggcgcac ctaagtcgtc attcagcttc
     3181 gggcgtatct gggatcagta cggcatgctg gtggtgtttg cggtgctctt tatcgcctgt
     3241 gccatttttg tcccaaattt tgccaccttc attaatatga aagggttggg cctggcaatt
     3301 tccatgtcgg ggatggtggc gtgtggcatg ttgttctgcc ttgcttccgg tgactttgac
     3361 ctttctgtcg cctccgtaat tgcctgtgcg ggtgtcacca cggcggtggt tatcaacctg
     3421 actgaaagcc tgtggattgg cgtggcagcg ggtttgttgc tgggcgttct ctgtggcctg
     3481 gtcaatggct ttgttatcgc caaactgaaa ataaatgctc tgatcacgac actggcaacg
     3541 atgcagattg ttcgaggtct ggcgtacatc atttcagacg gtaaagcggt cggtatcgaa
     3601 gatgaaagct tctttgccct tggttacgct aactggttcg gtctgcctgc gccaatctgg
     3661 ctcaccgtcg cgtgtctgat tatctttggt ttgctgctga ataaaaccac ctttggtcgt
     3721 aataccctgg cgattggcgg gaacgaagag gctgcgcgtc tggcgggtgt accggttgtt
     3781 cgcaccaaaa ttattatctt tgttctctct ggcctggtat ctgcgatagc cggaattatt
     3841 ctggcttcac gtatgactag tgggcagcca atgacgtcga ttggttatga gcttattgtt
     3901 atctccgcct gcgttttagg tggcgtttct ctgaaaggtg gcatcggaaa aatctcatat
     3961 gtggtggcgg gtatcttaat tttaggcacc gtggaaaacg ccatgaacct gcttaatatt
     4021 tctcctttcg cgcagtacgt ggttcgcggc ttaatcctgc tggcagcggt gatcttcgac
     4081 cgttacaagc aaaaagcgaa gcgcactgtc tgatgctttt ttccacaaca acttaacgtt
     4141 ttttcccacc acagccagcc gccacaacgg ttggctgttc ttcgttgcaa atggcgacct
     4201 ccgtcacact gtctatactt acatgtctgt aaagcgcggt ctgcgcaaca caataagaaa
     4261 agagaaggag gagagccggg tgacagaacc gttaaccgaa acccctgaac tatccgcgaa
     4321 atatgcctgg ttttttgatc ttgatggaac gctggcggaa atcaaaccgc atcccgatca
     4381 ggtcgtcgtg cctgacaata ttctgcaagg actacagcta ctggcaaccg caagtgatgg
     4441 tgcattggca ttgatatcag ggcgctcaat ggtggagctt gacgcactgg caaaacctta
     4501 tcgcttcccg ttagcgggcg tgcatggggc ggagcgccgt gacatcaatg gtaaaacaca
     4561 tatcgttcat ctgccggatg cgattgcgcg tgatattagc gtgcaactgc atacggtcat
     4621 cgctcagtat cccggcgcgg agctggaggc gaaagggatg gcttttgcgc tgcattatcg
     4681 tcaggctccg cagcatgaag acgcattaat gacattagcg caacgtatta ctcagatctg
     4741 gccacaaatg gcgttacagc agggaaagtg tgttgtcgag atcaaaccga gaggtaccag
     4801 taaaggtgaa gcgattgcag cctttatgca ggaagctcct tttatcgggc gaacgcccgt
     4861 atttctgggc gatgatttaa ccgatgaatc tggcttcgca gtcgttaacc gactgggcgg
     4921 aatgtcagta aaaattggca caggtgcaac tcaggcatca tggcgactgg cgggtgtgcc
     4981 ggatgtctgg agctggcttg aaatgataac cgccgcatta caacaaaaaa gagaaaataa
     5041 caggagtgat gactatgagt cgtttagtcg tagtatctaa ccggattgca ccaccagacg
     5101 agcacgccgc cagtgccggt ggccttgccg ttggcatact gggggcactg aaagccgcag
     5161 gcggactgtg gtttggctgg agtggtgaaa cagggaatga ggatcagccg ctaaaaaagg
     5221 taacattacg tgggcctctt ttaacctcag cgaacaggac cttgacgaat actacaacca
     5281 attctccaat gccgttctct ggcccgcttt tcattatcgg ctcgatctgg tgcaatttca
     5341 gcgtcctgcc tgggacggct atctacgcgt aaatgcgttg ctggcagata aattactgcc
     5401 gctgttgcaa gacgatgaca ttatctggat ccacgattat cacctgttgc cctttgcgca
     5461 tgaattacgc aaacgtgggg tgaataatcg cattggttt
//
LOCUS       DAZPCS010000201         5485 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZPCS010000201 DAZPCS010000000
VERSION     DAZPCS010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5485
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.204"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..1630)
                     /locus_tag="ACHUQ7_003656"
     CDS             complement(<1..1630)
                     /locus_tag="ACHUQ7_003656"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFR1136874.1"
                     /translation="MENSRIPGEHFFTTSDNTALYYRHWPTLQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKNSGPRGYSPSLMRSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFICRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKPW"
     gene            complement(1646..2541)
                     /locus_tag="ACHUQ7_003657"
                     /pseudo
     CDS             complement(1646..2541)
                     /locus_tag="ACHUQ7_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
     gene            complement(2541..3146)
                     /locus_tag="ACHUQ7_003658"
     CDS             complement(2541..3146)
                     /locus_tag="ACHUQ7_003658"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFR1136875.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLTALALSLFTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLICTAINRCRSVLLMSAER"
     gene            complement(3317..>5485)
                     /locus_tag="ACHUQ7_003659"
     CDS             complement(3317..>5485)
                     /locus_tag="ACHUQ7_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HFR1136876.1"
                     /translation="KQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLI
                     VLGNLTVKGNISTFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNP
                     STIKGSINARGVFINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWG
                     LINDEDALKEIFKRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEG
                     RDIATFDLAIMRTLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLG
                     YQPVVLGLLDREPLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQ
                     ELYKDAWLLPESIIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKL
                     AEMKHHGQLLRMTPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKS
                     PAYFCCRDMQVIHSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKN
                     NPKADSDAYETSADMILSELISHGEVDDQMLLNAAALIRLEDWDFLESALVSWDNLPA
                     VVLKELQQNTPRNDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDR
                     IWWSTLAKSNLTFFKFGALSNRHTPSAALAAEIDPEWWIVAMNNPRFPVDVLKARLKR
                     DPLLALELVNPELDLVRQLALNGKTRAIREQAMRKLDELY"
BASE COUNT         1581 a         1364 c         1141 g         1399 t
ORIGIN      
        1 cccacggttt accatcttta tgactggtta acaccccggc aatcagctcc agttgtgggt
       61 gccacggctg cccggtgtag atgagaatgc cacccggttc gatggcattg gctagacctg
      121 cgagtgagtt ttttacctgc tcattttcgg gaaaaagctc atataggcca gagacaatcg
      181 ccagcgtagg ccgcggagtt aacgcgctga gttcctccgg gttaaacgca tcgccctgtt
      241 caaaacgcac ccgcccagac attccccgtt gggcaatcat ctcttgcccc tgtgcaacat
      301 ttaactcact gtaatcacgt aacaaaatat cgcttacggc aggctcgttt gccagcgcat
      361 ccagtacata gcgtccatgc cctgcggcaa tgtcaaccac gcggacggct aaacctttgg
      421 cgtggagatc ggcaacggct tgtttaatca atatttgcaa atgggttttg cgctggcgaa
      481 taccgcgcca tccaacactg ttcaggtaga ttttgtcgat taaacgcccg aatgcgttac
      541 tgccttgcgg ttgattttga tagacataat ccagggaact gcctgaatca aagccggtac
      601 tcattccgag atgcaggccc gcagagtgcg caccgaacaa tttcatcgcc tttcgcataa
      661 agcgatacgc taaatcaacc ggcgataatg gcacgggtcc accagaaagg agccgccatc
      721 gatccgctga tggtcctgtg cggtcttcat gctgataatc aaatttttgc gatttgttag
      781 catataagcg gcagataaag ctttgcattt tttcaaatgc ctgcgccctg ttctcttcac
      841 ccaatgtgtc gtgataaaag cctggcagca gatgcagctc tttcagaggg ctacgtaatc
      901 tctgataaaa atcaatctgc ggttggcgat gcaccacata gtcatcacct gatatcagaa
      961 gttgcgtggg gagcgtaatc gccgccgcat cgctaacaat acgttcagac gttttataga
     1021 gatcgagcaa gatattgacg gcaatcgccc gtgtgatcag cggatcatta ttgaaactcg
     1081 ccacccgttg ccgatcgtgg gtcaaatagc gccctttcac ataggaatta ataaaaaata
     1141 gaccacgcag acgatgccat aacgccagcg caggacgtgc cagcggcaca tacaatttaa
     1201 ccttaaaggc cggagaagcc agcaccagcc cacgaattgc aggtgcataa tcatgaaccc
     1261 atgtggcaac aagcactgcg ccgacgcttt gcgcgatcac taccacctct tccagtccga
     1321 cctggctgtc gctggcagca aaacggacaa attcatcgac atcctgcact gagcgcataa
     1381 gagacgggct ataaccacgc ggccccgaat tttttccatg cccccgggca tcccaggcat
     1441 aaaaagcagt atcaggcatc gccagttcat caacgagatg ttgtagacga ccagaatgtt
     1501 catgcccgcg atgaaataag acgatgacct ttttcgcccc tggctgtaaa gtgggccagt
     1561 gccgataata caatgctgta ttatcactgg tggtaaaaaa atgttcccca gggatgcgtg
     1621 aattttccat tttctctatt ttccttcagt aacagcagta gcgaataaaa taaaaaaata
     1681 ccggagcggt gaaaattaat gagtcaatcc tgtcaaggag tccgccatgt cctagtaata
     1741 gttttccact atctttaaca ccaatatctc gtttgatggc tgacatcact acatcgccgc
     1801 aaaaaccact aataccaatt aacaaacccg ctaataatgc ctgtaatgta tttagcggtg
     1861 tcagcaacgg tccaataatc agtgacgcaa tcatggtggt gattacgccc cccagcagac
     1921 cttccagcgt ttttcccggg ctgactttgg ggaccacttt tcttctgccg caggatttcc
     1981 cccataaata ctgtgcaata tcgtttgact cggttaacgc caaaagaaat aacaccagta
     2041 acgccccgcc ctggatattt atggttggca acattaatag ccaggcggca tgactcaagg
     2101 caaaaacggt cattatccat ccccaaaaaa tggcgctcac ggtatgctga aaaccggagg
     2161 ggtcacccac aaaaactcgc caggtggcta atatcagaaa cccagtcaaa ggtataaata
     2221 acagaaacag ctcaaagcaa ttaaaaccga taagcaaata gttaagagga ataacccaat
     2281 ataaccaacg cgaaaaatgt acggatataa gcgtacaata ttctttcaat gccagaaaac
     2341 ttatcagcgt aaaaaacgtc aacgtcatcc agcgtgggcc tgaaatcacc agtgaaaaac
     2401 aaatgacgat gccccaccag gtacgaatac gtaatgtaac ttcatcccct tttctctcag
     2461 gtaaccgcca tagcagaaaa cggtttatca gcttgctaaa attaacaacg cgaaaagtgt
     2521 tgccagagat ttttccagca ttatctttca gcgctcataa gaagcacact ccgacaacga
     2581 ttaatcgcag tacagataag cagaattgaa gcaatgctcc ataaaagatt attccattgc
     2641 atccactgtg gataaatggc aacggccaga ccccacagac caaatattaa cgcgcgatca
     2701 cttttgccaa atggaccggc ataactgcga acaccgttaa tcgtctgcgc gagtaaaccg
     2761 caaaactcgg tcaatatggt gcaaaataac atgagtatca cgagtgatgc gttactttcc
     2821 ggtaaaaata aaaacggtaa atagagcgca atatcggaaa taacatcgcc agtctcattc
     2881 aaaatcgccc ccagacgtgt ttgctgattg cactcacgcg ccaacatgcc atccagcgca
     2941 ttgagcgcca tacggataaa aagcacgatg ggcaatagca aaaagaggat gggttgtgcc
     3001 accaacacca acagcaatcc ggtaaaaaga gaaagcgcca gtgccgtaag agtgatgtga
     3061 ttcgctgtaa cgtggtgctt ataaagccaa aacatcgtcg gccttaacag cgactgaaag
     3121 agcggtttta tctggtagag cgtcattatc aatccttgat aattcggttt gcgctatggt
     3181 gcatcaattc ccctgacgga gatatcgcaa cacgccgctt cattaaaaat tcagaatata
     3241 tcaaggtatt gggggctcac tgtcaggaat gattatcccc aataaatcaa gtagttaaaa
     3301 attattacgc tgttattcaa tacaactcat caagtttcct catcgcttgt tcccgtattg
     3361 cgcgcgtctt accgttaagc gccagctggc ggactaaatc cagttcaggg ttaacaagct
     3421 ctaacgccag caaaggatca cgtttcagcc tcgccttcaa tacatcaact ggaaagcggg
     3481 gattattcat cgccacaatc caccactcgg gatcaatttc tgccgccagt gccgcagagg
     3541 gggtgtggcg gttgctaagt gcgccgaact tgaaaaatgt gagattgctt ttcgccagtg
     3601 tactccacca tatacgatcc ggttgttttg ccagtacatc cagttgtgct aacgcatcgg
     3661 ggtcgagtgc ataataaaca cgtaatgctt catcaacctg cgcacgggag ctattttcct
     3721 gcctcagaaa aaacttcgcc caaatatcat tgcgtggcgt gttttgctgt aattccttga
     3781 gaacaacagc gggtaaatta tcccaactaa ctaatgcact ctctaaaaag tcccagtctt
     3841 ccaaacgtat cagggcggcg gcatttaata gcatctggtc atcaacttcg ccgtggctaa
     3901 tcagctcaga caaaatcata tcagcgctgg tttcatatgc atctgaatct gctttcggat
     3961 tatttttccg atgcgataaa gcacgttgcc aaattttttg tttcatcgac gtgctcagat
     4021 actgattttt cgccagatta ctccacccct caggatcgtt gaaacgtgtc aataaccgtt
     4081 gtaaagcagc gtcagagtga attacctgca tatcccgaca gcaaaagtag gcaggggatt
     4141 ttttctccat ccttttaaca aaattaaact gtaagttatc tggcaatgct aaaaataaca
     4201 cacgtattag atcatcgtca ttctcgaatt tttggtataa ataggctgca actttgtcac
     4261 tctcttgtgg cgtcatccgc aataattgac catggtgttt catctctgcc agcttgaacg
     4321 ctaagcttgt gacaacattg tttgatgaat cgttcgccaa ctgcattgcc tgattgtaag
     4381 tgagttgtcc tccagcaccg acctgacgaa tagaggaatc atcactgcgg atatagccat
     4441 caataatgga ttcgggcaat aaccacgcat ctttataaag ctcctgaatg atcatatccc
     4501 aatctctttt cgccagagtc atgacgatat ccagagggag attttcacgt agcgcaagat
     4561 tcaacggacc gacaccatca ggtagacgac tcattcgcgt cattatttcg acagagagcg
     4621 gttcacggtc gagcaacccc agaacaacag gctggtaacc caattgctga tcggtcaact
     4681 ccagaatctc attgtcaggc atatatctcg ccatactttc aatcgtttgt tcatcattgc
     4741 tattaataag ttgtttgcgt aggttagctg aaattccttt aagccttggg agagttcgca
     4801 ttatggcaag atcaaatgtc gcaatatctc gtccttcaaa tgcagcaacg ttgccttcat
     4861 ccagcatttg caattcctgc actaactcag gagaaggctt agggcgaaac agcgcttctt
     4921 ttttcatctg attacagaca ttccctgcca tgcgaatatt gcttcgctta aaaatttctt
     4981 tcagagcatc ttcatcattt attagacccc aataatatgc atcaggattt attatcatca
     5041 gcgctttttt gacattttca gtgtcatttt tgtcactcgc acggaccatg aattcactat
     5101 ttgtcgaaga cgcaacaaca ggtgcaatta tgtcattgat aaatacccca cgcgcattaa
     5161 tactcccctt aatcgtagac ggattatcgt aatatgagtc gataaatacc agtccacttg
     5221 catttatcga accggtaatt aacagtggtg aatccgtaac tatattcgtt gccgtcacgt
     5281 taccgagaat aacccatgga ttagaaagag agaaagtgga aatattacct ttgactgtca
     5341 aattgccaag aacaatcaat ttttgattac tctctatatt accttctacc actaaatcgc
     5401 cacgatgcac tttccagtcc gatgcagata aaccgaaggg gacaagattc acgctactgt
     5461 tgatagcttg ttcaacttgt tgttt
//
LOCUS       DAZPCS010000202         5398 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZPCS010000202 DAZPCS010000000
VERSION     DAZPCS010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5398
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.205"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..497
                     /locus_tag="ACHUQ7_003660"
     CDS             <1..497
                     /locus_tag="ACHUQ7_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="COX aromatic rich motif-containing protein"
                     /protein_id="HFR1136877.1"
                     /translation="EIAFPANTPVYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHL
                     IANEPGTYDGISASYSGPGFSGMKFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEK
                     LAAPSEYNQVEYFSNVKPDLFADVINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHA
                     ESAH"
     gene            519..2510
                     /gene="cyoB"
                     /locus_tag="ACHUQ7_003661"
                     /pseudo
     CDS             519..2510
                     /gene="cyoB"
                     /locus_tag="ACHUQ7_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
     gene            2500..3114
                     /locus_tag="ACHUQ7_003662"
     CDS             2500..3114
                     /locus_tag="ACHUQ7_003662"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFR1136878.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            3114..3443
                     /locus_tag="ACHUQ7_003663"
     CDS             3114..3443
                     /locus_tag="ACHUQ7_003663"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFR1136879.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            3455..4345
                     /gene="cyoE"
                     /locus_tag="ACHUQ7_003664"
     CDS             3455..4345
                     /gene="cyoE"
                     /locus_tag="ACHUQ7_003664"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFR1136880.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPSVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            4529..4840
                     /locus_tag="ACHUQ7_003665"
     CDS             4529..4840
                     /locus_tag="ACHUQ7_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFR1136881.1"
                     /translation="MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            4824..5373
                     /locus_tag="ACHUQ7_003666"
                     /pseudo
     CDS             4824..5373
                     /locus_tag="ACHUQ7_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1177 a         1344 c         1425 g         1452 t
ORIGIN      
        1 atgaaatcgc tttcccggcg aacactccgg tgtacttcaa agtgacctcc aactccgtga
       61 tgaactcctt cttcattccg cgtctgggta gccagattta tgccatggcc ggtatgcaga
      121 ctcgcctgca tctgatcgcc aacgaacccg gtacttatga cggtatctcc gccagctaca
      181 gcgggccggg cttctcaggc atgaagttca aagctattgc aacaccggat cgcgccgaat
      241 tcgaccaatg ggtcgcaaaa gcgaaacagt cgccgaacac catgtctgac atggctgcgt
      301 tcgaaaaact ggccgcgcct agcgaataca accaggtgga atatttctcc aacgtgaaac
      361 cagacttgtt tgccgatgtg attaacaagt ttatggctca cggtaagagc atggacatga
      421 cccagccaga aggtgagcac agtgcacacg aaggtatgga aggcatggac atgagccacg
      481 cggaatccgc ccattaaagg ggttgaggaa gaataaagat gttcggaaaa ttatcacttg
      541 atgcagtccc gttccatgaa cctatcgtca tggttacgat cgctggcatt attttgggag
      601 gtctggcgct cgttggcctg atcacttact tcggtaagtg gacctacctg tggaaagagt
      661 ggctgacctc cgtcgaccat aaacgcctcg gtatcatgta tatcatcgtg gcgattgtga
      721 tgttgctgcg tggttttgct gacgccatta tgatgcgtag ccagcaggct cttgcctcgg
      781 cgggtgaagc gggcttcctg ccacctcacc actacgatca gatcttcacc gcgcacggcg
      841 tgattatgat cttcttcgtg gcgatgcctt tcgttatcgg tctgatgaac ctggtggttc
      901 cgctgcagat cggcgcgcgt gacgttgcgt tcccgttcct caacaactta agcttctggt
      961 ttactgttgt tggcgtgatt ctggttaacg tttctctcgg cgtgggcgaa tttgcgcaga
     1021 ccggctggct ggcctatcca ccgctatcgg gaatagagta cagtccggga gtcggtgttg
     1081 attactggat atggagtctc cagctatccg gtataggtac gacgcttacc ggtatcaact
     1141 tcttcgttac cattctgaag atgcgcgcac cgggcatgac catgttcaag ataccagtat
     1201 ttacctaggc atcactgtgc gcgaacgtac tgattattgc ttccttccca attctgacgg
     1261 ttaccgtcgc gttgttgacc ctggatcgct atctgggcac ccatttcttt accaacgata
     1321 tgggtggcaa catgatgatg tacatcaacc tgatttgggc ctggggccac ccggaagttt
     1381 acatcctgat cctgcctgtt ttcggtgtgt tctccgaaat tgcggcaacc ttctcgcgta
     1441 aacgtctgtt tggttatacc tcgctggtat gggcaaccgt ctgcatcacc gtgctgtcgt
     1501 tcatcgtttg gctgcaccac ttctttacga tgggtgcggg cgcgaacgta aacgccttct
     1561 ttggtatcac cacaatgatt atcgccatcc cgaccggggt gaagatcttc aactggctgt
     1621 tcaccatgta tcagggccgc atcgtgttcc attctgcgat gctgtggact atcggtttta
     1681 tcgtcacctt ctcggtgggc ggtatgactg gcgtgctgct ggcagtaccg ggcgcggact
     1741 tcgttctgca taacagcctg ttcctgattg cgcacttcca taacgtgatc atcggcggcg
     1801 tggtcttcgg ctgcttcgca gggatgacct actggtggcc taaagcgttc ggtttcaaac
     1861 tgaacgaaac ctggggtaaa cgcgcgttct ggttctggat catcggcttc ttcgttgcct
     1921 ttatgccgct gtatgcgctg ggcttcatgg gcatgacccg tcgtttgagc cagcagattg
     1981 acccgcagtt ccacaccatg ctgatgattg cagccagcgg tgcagtactg attgcgctgg
     2041 gtattctctg cctcgttatt cagatgtacg tttctattcg cgaccgcgac cagaaccgtg
     2101 acctgactgg cgacccgtgg ggtggccgta cgctggagtg ggcaacctct tccccgcctc
     2161 cgttctataa ctttgccgta gtgccgcacg ttcacgaacg tgatgcattc tgggaaatga
     2221 aagagaaagg cgaagcgtat aaaaagcctg accactatga agaaattcat atgccgaaaa
     2281 acagcggtgc aggtatcgtc attgcagctt tctccaccat cttcggtttc gccatgatct
     2341 ggcatatctg gtggctggcg attgttggct tcgcaggcat gatcatcacc tggatcgtga
     2401 aaagcttcga cgaggacgtg gattactacg tgccggtggc agaaatcgaa aaactggaaa
     2461 accagcattt cgatgagatt actaaggcag ggctgaaaaa tggcaactga tactttgacg
     2521 cacgcgactg cccacgcgca cgaacacggg caccacgatg caggcggaac caaaatcttc
     2581 ggattttgga tctacctgat gagcgactgc attctgttct caatcttgtt tgctacctat
     2641 gccgttctgg tgaacggcac cgcaggcggc ccgacaggta aggacatttt cgaactgccg
     2701 ttcgttctgg ttgaaacttt cttgctgttg ttcagctcca tcacctacgg catggcggct
     2761 atcgccatgt acaaaaacaa caaaagccag gttatctcct ggctggcgtt gacctggttg
     2821 tttggtgccg gatttatcgg gatggaaatc tatgaattcc atcacctgat tgttaacggc
     2881 atggggccgg atcgcagcgg cttcctgtca gcgttctttg cgctggtcgg cacgcacggt
     2941 ctgcacgtca cttctggtct tatctggatg gcggtgctga tggtgcaaat cgcccgtcgc
     3001 ggcctgacca gcactaaccg tacccgcatc atgtgcctga gcctgttctg gcacttcctg
     3061 gatgtggttt ggatctgtgt gttcactgtt gtttatctga tgggggcgat gtaatgagtc
     3121 attctaccga tcacagcggc gcgtcccatg gcagcgtaaa aacctacatg acaggcttta
     3181 tcctgtcgat cattctgacg gtgattccgt tctggatggt gatgacaggg gctgcctctc
     3241 cggccgtaat tctgggaaca atcctggcaa tggcagtggt acaggttctg gtgcatctgg
     3301 tgtgcttcct gcacatgaat accaaatcag atgaaggctg gaacatgacg gcgtttgtct
     3361 tcaccgtgct aatcatcgct atcctggttg taggctccat ctggattatg tggaacctca
     3421 actacaacat gatgatgcac taagagcggc ggttatgatg tttaagcaat acctgcaagt
     3481 aacgaaacca ggcatcatct ttggcaacct gatctcggtg attgggggat tcctgctggc
     3541 ctcaaagggc agcattgatt atcccctgtt tatctacacg ctggttgggg tgtcactggt
     3601 tgtggcgtcg ggttgtgtgt ttaacaacta catcgacagg gatatcgaca gaaagatgga
     3661 aaggacgaag aatcgggtgc tggttaaagg cctgatctct cctgctgtct cgctggtgta
     3721 cgccaccttg ctgggtattg ctggctttat gctgctgtgg tttggcgcga atccgctggc
     3781 ctgctggctg ggggtgatgg gctttgtggt ttatgtcggc gtctatagcc tgtacatgaa
     3841 acgccactca gtctacggca cattgattgg ttcgctctcc ggcgctgcgc cgtcggtgat
     3901 tggctactgt gcagtaaccg gcgagtttga tagcggcgca gcgatcctgc tggctatctt
     3961 cagcctgtgg cagatgcctc actcctatgc catcgccatt ttccgcttta aggattacca
     4021 ggcggcaaac attccggtat tgccagtggt aaaaggcatt tcggtggcga agaatcacat
     4081 cacgctgtat atcatcgcct ttgccgttgc cacgctgatg ctctctcttg gcggttacgc
     4141 tgggtataaa tatctggtgg tcgccgcggc ggttagcgtc tggtggttag gtatggctct
     4201 gcgcggttat aaagttgctg atgacagaat ctgggcgcgc aagctgttcg gcttctctat
     4261 catcgccatc actgccctct cggtgatgat gtccgttgat tttatggtac cggactcgca
     4321 tacgctgctg gctgctgtgt ggtaacaaaa cctctctatt aaaaaggtgc tacggcacct
     4381 ttttattgtc tgagatcaga gatacggcta ataaccgtat ctcttgacag agtgagcaga
     4441 tgcacataaa ttaatcatac ggttgatagc cgtattatgt atgagatcga ctccctacga
     4501 tttcattcag ttaatcaaag aagtcataat gcctggaaaa acagccgctc tatacgatgt
     4561 cgataaaacg ctgaaaaatg cgcgggtcga actcaaaact agtcccgacg ccaaaaacaa
     4621 acttcgtgaa gctgcgcaag ccgttggggt agatttaagc gcttttattt tgagcgctgc
     4681 tatggaacgc gccgaaagtg tgcttgataa ccaacgtcgc cgtgagcttt cgaatcaaag
     4741 ctgggaactg atgaaccaac tcatcgctga acctgctcaa ccgacgctcg ccctcaaggc
     4801 gttaatgaaa aggaaaaaca gcgatggtcg acaagcatga agagattact ctgcccatag
     4861 tcctctcctg taattatcag tctgatatta cttatcctgg gcaaaaacag tttgattgcg
     4921 gtaaccctgt tatcgataaa tttgtacgcg ctacgcgcat cgctaaagaa aagtgtgcgt
     4981 aatagcgact gtgcggctaa agcacttatt gacagacaaa gtggtgaact gattggcatc
     5041 tgtactttta cggcatattc gctggaaaga caacgcgttt ctggcgtcct tcagggttca
     5101 caaccttcag aaattggtgt tgtcagatta gtcatgttgg ggggagcgcg gaagtatcaa
     5161 aagcggggct ttggtcagga cctactatgt gatttttttg aacatgtaaa aaaaattcac
     5221 ccggcattac caattaaagg ggtttatctt gatgctgacc ctgccgccat taacttttat
     5281 gctcgtctcg gctttgttca gctttcagcg acaccaaatg cttttggtgc tgtacctatg
     5341 tttttggcga ttcagcatat tctcgcggct tagattatta tcgccacccg ccagggca
//
LOCUS       DAZPCS010000203         5374 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZPCS010000203 DAZPCS010000000
VERSION     DAZPCS010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5374
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.206"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(99..581)
                     /locus_tag="ACHUQ7_003667"
     CDS             complement(99..581)
                     /locus_tag="ACHUQ7_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1136882.1"
                     /translation="MIKPFLMTVAILTSCCAQAKILTNEEIVSRFISNVKTSADNDVD
                     LDTDGFRQLRISCPSPSASGAIILRKASYEFGKSIGVFDFANNASPATMTFLIPIYKD
                     DDFDSDTVGFTFAFKMPRGQFFVDVKENGNIRAGVNVNGESGVTYENCKLNMKDINDD
                     "
     gene            complement(649..2598)
                     /locus_tag="ACHUQ7_003668"
     CDS             complement(649..2598)
                     /locus_tag="ACHUQ7_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR1136883.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLSLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDTTAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKEHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(2683..3018)
                     /locus_tag="ACHUQ7_003669"
     CDS             complement(2683..3018)
                     /locus_tag="ACHUQ7_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFR1136884.1"
                     /translation="MAMNVTEIVLKKPVTAHNEMLHVLELREPTYDEIEALGFPFIIS
                     GEGSIKLDSQVALKYIPLLAGIPCSSAAQMAKLDIFKTSMQILRFFTQSETGSTSGND
                     STMLPGSGN"
     gene            complement(3018..3374)
                     /locus_tag="ACHUQ7_003670"
     CDS             complement(3018..3374)
                     /locus_tag="ACHUQ7_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFR1136885.1"
                     /translation="MAKIAGICFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(3374..4870)
                     /locus_tag="ACHUQ7_003671"
     CDS             complement(3374..4870)
                     /locus_tag="ACHUQ7_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFR1136886.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHVYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVEKYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            complement(4867..5028)
                     /locus_tag="ACHUQ7_003672"
     CDS             complement(4867..5028)
                     /locus_tag="ACHUQ7_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HFR1136887.1"
                     /translation="MFVKPLKGRSVPDPARGDLLPSEGRNVEESSYWFRRIAAGDVVR
                     VKQDKAKKS"
     gene            complement(5038..>5374)
                     /locus_tag="ACHUQ7_003673"
     CDS             complement(5038..>5374)
                     /locus_tag="ACHUQ7_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1136888.1"
                     /translation="RDEKGQWAAYDAVHDVRRELWKALLGWMPDPQGGEIVYVGGTLL
                     DLNRYELYYQFDFTAKYEITEEDTRQAEDVNALPDLSLLSIDVDYIDPGTGPDGDIEH
                     HLEMRFPQN"
BASE COUNT         1284 a         1565 c         1155 g         1370 t
ORIGIN      
        1 cagtaagttg gagtcattac caagaaagag aaggtaaaga aggtaagtcc atactgtctc
       61 cactataaaa aaaccgccaa tttctggcgg cttatatatt aatcatcatt aatatctttc
      121 atattcaatt tgcaattttc gtaggtcact cccgactcgc cattaacatt aactccagct
      181 ctgatattcc cgttttcttt tacatctaca aaaaactggc ctcttggcat cttgaacgca
      241 aatgtaaaac caacagtatc tgaatcaaaa tcatcatctt tatatattgg gatcaaaaat
      301 gtcatcgtcg ctggtgatgc attgtttgca aaatcaaata cacctatact tttgccgaat
      361 tcataagaag cttttctcaa tataatcgca ccgctggcag aaggggatgg gcacgatatc
      421 ctcagttgtc tgaatccatc tgtatccaag tcaacgtcat tatctgcaga tgttttaacg
      481 ttactaataa atcgtgaaac aatctcctca tttgttagta ttttcgcctg agcacagcaa
      541 gaggttaata ttgcaaccgt cattaaaaat ggctttatca caaaacaaac tccattgtta
      601 gcatgttacg cgataatttt gcctcatctt tcaccacaaa aaaagaactc accttttaaa
      661 tggagaatac ccaacatcat gggtgatttt cataaaggga tcgcctgttt tcggcaaatc
      721 gataacgcgc attccaggcg gcgcgttgtc aaacgtcacc ttaagttcgc tgcgggttgc
      781 cggtgaagat gcaaggctta acagctgatt cctttccagt gatacattcg gggtgtagcg
      841 tttatcggga gataaatacc catcaccacg tggcatctgc catccggtca tgtcatagac
      901 aaaatcatgg aactgtttgc cccactcgtc gaacttatca ttcagaccaa atccactgtt
      961 cagtgcatca gccacaaaat ttttaacaag ccagggatgt tctttttcaa actcctgcgc
     1021 catcatgccc agttcaagca atccaccaat cagactgatc tttccgaggc cctttaattt
     1081 cagccctgtt tttcctgccg ccgttgtcca ttgattctgg gcgactgttg ccgcacgaat
     1141 acttgccacc gccttcacac caatatacag ggcagaaacc gttgctattg tttttactgc
     1201 ctgttcccat ccccccattg ctctgacaac ctgatcaacc tcctgccaga cttttttcac
     1261 aaccggaccg accgtttccc agttatcaat tatgagatat gcaccaccga cgagaagcgc
     1321 aattaatccc tttgccggcg tcatattcat cacgctaccc atgatttttg ttatgcgtgt
     1381 caatgtgcca atcgccacgc ccatcgtcag taatgccgca ccagttttcg cgatggtttt
     1441 tacgacctca ggattttctc tgacaaacgt gcgcacttct tccagaaaag gcttcatctc
     1501 ttttatgcct tcattgagtg aaggcaggaa ggtttccccc agcgtggaag aaatcgcatt
     1561 gatctggttc tgcagcagca acaactgatt ttctgtcgtc gcggcacggg cggcatattc
     1621 tttctgcatt gagccgccat actgctgcgc atccgccaca cgattaaagt tggttcgcaa
     1681 caaatccaga ttcgtgagca gcggcgcaat ggcccccagg gattccttcc cgaacagcgc
     1741 tttcagcaca gcagcctgtt tttctttcgg cacttttgcc agagaatcca gtacgtgcag
     1801 catggccccg cgggcgtctt tctgcatatc cgccgccagt tttttcgggc taatgcgcaa
     1861 cgagcggagc acttttttct gcgatttggt ggcagaatcc ctcgctgtca gcgacaacat
     1921 aaaattcttt atccccgtcg cagcaatttc tgactccacc cccattccgg caatggttgc
     1981 ccccattgcg gcaatctctc cggaggccac acccgcaaca ctgcctaaag ggccaatacg
     2041 ggtcacaaca tcagaaatct ttttcgcact tgcaggaccg gtattgccaa gatagttaat
     2101 cttatccgca agccctgcca cctctccctg tgtcagttta aaggcagtgc gccactgcgc
     2161 catcatctga ccggattctt ctgccgtggt gtcaaaggcc acgcccatct tcacggcatc
     2221 gtcagtaaac tgcatcagct cgtcacgggc gataccagcc tgaccacccg ccgccacaat
     2281 ttcggcgata ccttccgccg acatgggcag ttctgttgac aggtcgcgca cctgctccgt
     2341 cattgcctta aacgcttccg gcgtatccag accatccacc actttccgga catccgccat
     2401 ttttgattca agggcaatgg cagatttgac cgggagcgcc agcgccccca gtactgcggt
     2461 tccggcaccc gctgcgccca gtgacaggct ggcaaactcc tttttaaaac ctttcagctg
     2521 gcgctgcatt cccttcagcg gtgccgatac cttatccacg gcagtgatga tcgccttcag
     2581 ctgaaaactg tcagccatgc ttcatctcct cgttaatgcg gacggcctcg gcttccagtt
     2641 ctgtaaactg cgaaacagcc acccgtcgca gctccagtgg attcagtttc cagaaccagg
     2701 caacattgta gagtcgtttc cggaggtgct tcccgtctcc gactgggtaa aaaaacgcag
     2761 gatctgcatg ctggtcttaa aaatatccag ttttgccatc tgcgccgccg atgaacacgg
     2821 gatccccgcc agcaacggga tatatttcag tgccacctgg ctgtccagtt taatactgcc
     2881 ttcaccggaa ataatgaaag gaaaacccag cgcctcgatt tcgtcatacg tgggctcgcg
     2941 caactccagc acatgcagca tttcgttatg cgctgtcacc ggttttttta aaactatttc
     3001 tgtaacgttc attgccatta ctgataccct ccctcttcgc cgtggaattc aagatctgcc
     3061 gtgccttctt cggcattatg attagcctct ccgtgcagcc atgccgccga aagcacatac
     3121 accatgccgt ttgccagctc agcggtaatc gtcatctggt ctgaggtggt aattttgtcg
     3181 accggaaagt ttttgggcac tttaaacgtg cccttaacgt aaggtgaccg ccaggtctcc
     3241 ttgtaatcca catcccctgc catgccgacg atgtcatccc ggacattggt gttcatcggc
     3301 acttcaatgc cacctgtcag cgataactgc tgaccatcca ctttaaaaaa acagattccg
     3361 gcgatctttg ccattatgct gactcctctg aatactgaag acggaactgg ttaaccaccg
     3421 caaagacacg taactgatta acataatccg gcgggaacag cgtattcagt cggttcgggt
     3481 tatctgcatc acgctcaact atcagatact gtttaaacag atcgtatttt tccacaatac
     3541 cggcacgctc catctgacga tatgtcgcca gaagttcccc tttgataacg gcaggagtga
     3601 caatcgcctg ccccggacca aaacgggtac catcatttgc cagcttgtga cgtccgtact
     3661 tgcttgtgat gaccgatttc agtttgcgaa gaacatatgc actggtatgc agagtttcac
     3721 tgtccagata gctgttgtct gccacgccat acgcattctt tttgtaggtg gttacggaac
     3781 gctggatctg caacgtgccg ccttccacat aagccgtcgc cacgccgtga gataaaaggg
     3841 tctgctgctc tgtcatgatg aatcgcttac ctttcggtgc cggaagcatc cccaccagct
     3901 cccccgtctg tgtcggacgg gcaggatcat tccggataaa taccgcttca cgggcaaggc
     3961 gactggcaac cagttcatcg acaggcgact gggtttcttt ttcgtaaccg gcaagcgtga
     4021 tatgttgctg attatgcata tctccggcac caaccagctc tgacagcgtt cccagttttg
     4081 cggtatagac atgcccgtat aactggcgcg cataactcca gcgaccgctg ctgtcattca
     4141 tttcggtcat catcatattg atggaggcgg catcgttgaa cggcagaccg ataaagtcga
     4201 atgcctcatc gcccatagca gcaacagcgg cggtaagatc aggcgctccg ctaccggcag
     4261 caccgacttc cgtcaccacc cgaagccctg ctggcaaaat ctcaccacca ccagaaccat
     4321 aataattcag acagaccggt aactcattac catacagccc cttatgacgg gcagtcagcg
     4381 tcaccacgcc tgcatcagat gacgccgtaa acggcagggt gataaccccg tttaccgctt
     4441 ccttaatcgc ggtggcaacc gcagtggcat catcgccatt caccacaggc acctgtacac
     4501 gggagcgccc gacatacaga ctcagggtgc cgctttcctc tgcttctccg gcaaccgtca
     4561 ccctgaccgt cgccgccgcc cctctggctt ccggtactgc aataacatac agttcaccga
     4621 aaggatctgt ctgacggtag acttccacca tacgcgccag ctggctcccc gccccacaaa
     4681 tctggcgggc ataatctgcc gatggcatca gcaccaggct gttaacctca atggctgcat
     4741 cgttgctggc atgcccgatc agtaatgcag gcgcgctggt caccgctgta tttgccgcag
     4801 aattgttcat ctcggcgtag aacaacggca ccagcgtatt cgacggaaca gtattaaaac
     4861 ttatcgtcat gattttttag ccttatcctg tttaacacgt accacatcac ccgccgctat
     4921 acggcggaac cagtagctgc tttcttccac atttcgccct tcggaaggca aaaggtctcc
     4981 acgggcaggg tcaggaaccg accgcccttt caggggtttc acaaacatga gggactctta
     5041 attctgaggg aaacgcattt ccagatggtg ctcaatgtca ccatccgggc cagtaccagg
     5101 atcgatgtaa tccacatcaa tactcagcag ggataaatcc ggcagggcat tcacgtcctc
     5161 tgcctgtcgc gtgtcttctt ccgtgatttc atacttcgcc gtaaaatcaa actggtaata
     5221 cagttcgtaa cggttcagat ccagcagggt gccaccgaca taaacaatct cgcccccttg
     5281 cgggtccggc atccatccaa gcagggcttt ccagagttct ctccggacat catgaacggc
     5341 gtcataggct gcccactgcc ctttttcatc acgc
//
LOCUS       DAZPCS010000204         5358 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZPCS010000204 DAZPCS010000000
VERSION     DAZPCS010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5358
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.207"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..171
                     /locus_tag="ACHUQ7_003674"
     CDS             <1..171
                     /locus_tag="ACHUQ7_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1136889.1"
                     /translation="IRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTP
                     KRYRKKLTEFNQ"
     gene            276..1628
                     /gene="celF"
                     /locus_tag="ACHUQ7_003675"
                     /gene_synonym="chbF"
     CDS             276..1628
                     /gene="celF"
                     /locus_tag="ACHUQ7_003675"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFR1136890.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDCREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKVSGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            1641..2399
                     /gene="chbG"
                     /locus_tag="ACHUQ7_003676"
     CDS             1641..2399
                     /gene="chbG"
                     /locus_tag="ACHUQ7_003676"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFR1136891.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(2446..4707)
                     /gene="katE"
                     /locus_tag="ACHUQ7_003677"
     CDS             complement(2446..4707)
                     /gene="katE"
                     /locus_tag="ACHUQ7_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFR1136892.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKAMK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSTDGSFMDELLTLMAEHR
                     VWSRIPKIDKIPA"
     gene            4890..5153
                     /gene="cedA"
                     /locus_tag="ACHUQ7_003678"
     CDS             4890..5153
                     /gene="cedA"
                     /locus_tag="ACHUQ7_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFR1136893.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
BASE COUNT         1322 a         1190 c         1402 g         1444 t
ORIGIN      
        1 atccgtatta attttgcaaa aaaacaactg gaaatgacca actattccgt gacggatatt
       61 gcgtttgagg ccggttatag tagcccgagt ttgtttatta aaacgtttaa gaaattaacg
      121 tcctttacgc ctaagagata tcgtaagaaa ttgactgaat ttaatcagta agtcgttata
      181 cctgacaatt cacatatctg tcctgttgct ggattattta tgtccggggg ggcagatatg
      241 ccagatatca gtattctgta ctgaagggag aaattatgag ccagaaatta aaagtcgtca
      301 ctattggtgg cgggagcagc tataccccgg agttactgga aggatttatt aagcgttatc
      361 acgaattgcc ggtcagcgaa ttatggctgg tggatgtcga gggtggcaaa gctaaactgg
      421 atattatttt cgatctctgc caacggatga ttgataacgc tggcgtcccg atgaagcttt
      481 ataaaacgct ggattgccgc gaagcattga aagatgctga tttcgttact acccaactgc
      541 gcgttggcca attaccggcg cgtgaactgg atgaacgtat tccattaagt catggttatc
      601 ttggtcagga aaccaacggc gcgggcggtt tgtttaaagg tctgcgtacc attccggtga
      661 tttttgacat cgtaaaagat gtcgaagaac tttgtccgaa tgcatgggtg attaacttca
      721 ctaacccggc gggaatggtt actgaagccg tttatcgtca taccggattt aaacgcttta
      781 tcggcgtgtg taatattccg atcggcatga agatgtttat tcacgatgtt ctgatgctga
      841 aagacagcga tgatttatct atcgatttgt ttggcctcaa ccatatggtg ttcattaagg
      901 atgtgctggt aaatggcaag tcgcgctttg ccgaattgct tgatggtgtg gcatccgggc
      961 agttaaaagt atctggcgtt aaaaatattt tcgatctgcc atttagcgaa ggcttaattc
     1021 gttctctgaa tctgttgccg tgttcttatt tgctttatta cttcaagcaa aaagagatgc
     1081 tggctattga aatgggcgaa tactacaaag gcggcgcacg agcgcaggta gtacagaaag
     1141 tcgagaaaca actttttgag ctgtataaaa acccggagtt gaaagttaag ccgaaagaac
     1201 tggaacagcg cggtggggct tattactctg atgcagcgtg cgaagtgatc aacgctatct
     1261 acaacgacaa gcaagctgaa cattacgtta atatcccgca tcatgggcat attgataata
     1321 ttccggcaga ctgggcggta gaaatgacct gtaagctggg gcgcgatggc gcgacgccac
     1381 atccgcgcat tacgcatttc gatgataaag tgatggggct gattcacacc attaaaggct
     1441 tcgagattgc tgccagcaac gccgcactta gcggagaatt taacgatgtg ttactggcgc
     1501 taaaccttag tccgttggtg cattccgatc gcgatgctga gctgctggca cgcgagatga
     1561 ttctggcgca cgagaaatgg ctgccaaatt ttgccgactg catcgcagag cttaaaaaag
     1621 cacattaacc gaggctgatt atggaacgct tactgattgt taatgccgat gattttggct
     1681 taagcaaagg tcagaactac ggcattatcg aggcctgtcg caatgggatt gtcacatcga
     1741 cgacggcgct ggtgaatggg caggctattg accatgcggt gcagttgagt cgtgatgaac
     1801 cgagtctggc catagggatg cactttgtcc ttactatggg caagccactg acagctatgc
     1861 cggggttaac ccgcgatggt gtgctgggaa aatggatctg gcagttggca gaagaagatg
     1921 ctttaccgct ggaagaaatt actcaggagc ttgccagtca gtatttgcgt ttcattgagc
     1981 tatttggacg caaacctacg catcttgata gccatcatca tgtgcatatg ttcccgcaga
     2041 ttttcccgat tgttgccagg tttgcggctg aagaggggat tgcgttgcgc atcgaccgtc
     2101 agccgctatc taacgctggt gatttaccgg ctaatctgcg cagttcgcag ggattcagta
     2161 gcgcgtttta tggtgaagag attagcgagg cactgttcct gcaagtgctg gatgacgcaa
     2221 gccatcgggg tgatccttcg ctggaggtga tgtgtcatcc ggcgtttatc gataatacta
     2281 tccgtcagag tgcttactgt ttcccacggt taacggagct ggaagtgctg acatcagcgt
     2341 cgttgaaata tgcaattgct gagcgtggtt atcggttggg tagttatctt gatgtgtaag
     2401 taaggtggta aggtcactcc ccaggggacg tgaccttatc catatttacg ccggaatttt
     2461 gtcaatctta ggaatgcgtg accacacgcg gtgttctgcc atcagcgtta acagttcatc
     2521 cataaaacta ccgtcggtgc tgtcagcttc cacaatccct tcttcaccct ggtcagctac
     2581 cttgattgtt gctttaaact tgcgcgcgtc tcccgccagc gcaatcggtt taaggtgttt
     2641 gtaggcttcc atcagatagt agttggcatc gccgttgtca gcgatatccg cgatattgcc
     2701 gcaagggaca atgaccgcat cgaccgtcag cgaaggtgca ccggcaaagg tagcggctat
     2761 aggcaacacc gtaccgtcat ccgcagtcac ttcacccatt cgggagtaga gcagtttggc
     2821 atgaacgcct ttggccttca tggccttgag aatggccaga aggtctgccg atctcacttc
     2881 atcattaagt aaaatcgcta ccacgcgacc tttcacatca ccgtcaggaa tggcgtacaa
     2941 acttaaggat ggatcctttt tcagaccgtt gacgtccgga ggtggggtga tattcagctg
     3001 gtcgtcagtc agttcgatac cgagattttt cgccaccgcc tgggccagag tgagatcaat
     3061 atgcgccagc tggtcaacaa cgcgctcacg aatatacgga cgaacgactt tgcttaactc
     3121 aaaactgaaa ccatcgacaa tatggcgctg ctcgaatggc gtctgactta gccagaacag
     3181 acgcggatgg gaataatatt cgccaaacga tgggctgcgc tcgcgaactt tattgccttc
     3241 cacgcgctct tggtatgatt caaaaccgcc gcgtttcggc cccggtggtg tttcgcgcgg
     3301 ccagttatcg ttgatcgagt tcggttcata attcgccggg ttagtgtcga tccccatacg
     3361 atgcatgccg tcacgctgga aattatggta agggcaggtc gggcggttaa tcggaatctc
     3421 atggaaattc ggcccaccaa gacgactgat ttgtgtatcg gtataggaga acaaacgtcc
     3481 ctgcaacagg ggatcgttgg tgaagtccag accaggaaca atatgacctg gatggaatgc
     3541 tgcttgttcg ttttcggcaa agaagttatc cggattgcga ttgagcacca ttttgccgac
     3601 acgctgaacg ggcaccagtt cttccgggat cagtttggtt ggatcgagaa gatcgaagtc
     3661 gaacttgaat tcatcttctt caggaatcaa ctggaagccc agttcgtatt ccggaaaatc
     3721 gcctgcttca atggcttccc acaactcgcg gcggtggaag tccgggtcac gtccggtgag
     3781 tttttgtgct tcatcccaaa cgagtgaggc tttacctgcc agtggtttcc agtggaaacg
     3841 tacaaacgtt gccttccctt cggcattaat caggcggaag gtgtgaatac cgaagccttc
     3901 catggtgcgg taactgcggg ggatgccgcg atccgacatc gcccacatca cgttgtgcag
     3961 agtttcaggt tgcagagaaa cataatccca gaaagtatcg tgggcacttt gcccttgtgg
     4021 aattgcccag tgcggttctg gttttaccgc atgaacaaaa tcggggaatt tatgcgcatc
     4081 ctggataaag aagattggcg tgttattgcc aacgaggtca aaaataccct cttcagtata
     4141 gaacttggtg gcaaaaccac ggatatcacg cacggtatcg gcagagccag cgccaccctg
     4201 aacggtagag aacctaacaa atactggggt gattttgttc ggatctgaga ggaaatccgc
     4261 tttggtgata tcgcttaagc ttttatatgg ctggaaataa ccgtgagctg ctgagccgcg
     4321 tgcatgaaca atacgttccg ggatgcgctc atggtcaaag tgggtgattt tctcgcgcag
     4381 aataaaatct tccagcagcg ttggaccacg gttaccggca cgcagtgagt tttgatcatc
     4441 ggcgatgcgc acgccctgat tagtggtcag cgcataattt tcactgcctt tgcgtacgtc
     4501 ttccagagaa ttaagttttt cgttacgtgt atcaggggct ttcaggctcc caggggcggt
     4561 aggttgtgca cctggcggtg ttggttcagc cgctggacga tgagatccgt cctcaggtgc
     4621 cagtgagtcc atccccggtt tcgcttcgct ggaatcgtgt agtggtgact ggtgctgatg
     4681 tgggttcttt tcgttatgtt gcgacattga actcgtctcc ttaatttatt actgaaaggg
     4741 ccgctgtttt ggtcaaaaaa ctctctaact atagaccacc agccagatcc cgcttcggag
     4801 actattctgg aaacggtgtt atgaaataca cgcaaagcgg gacgtacagg ggctaaatcg
     4861 gctaaactac agtttttcta atttttaata tgcgtttagt gaagccagta atgaagaaac
     4921 cgctccgcca gcaaaaccgc cagattatta gctatgtccc acgcacggaa cccgcgccgc
     4981 cagaacatgc gataaagatg gattcgtttc gtgatgtctg gatgctgcgt ggcaaatatg
     5041 ttgcgtttgt actgatggga gagtcatttc tgcgctcacc ggcgtttact gtgcctgaat
     5101 ctgcccaacg ttgggcaaat cagatccgcc aggaagggga agtgactgag taaggattac
     5161 atacctcccg gttttgccgg gagttacagg acctgaaatt ctgcatttcg ttgctacatt
     5221 cattcaaacc acctgtaaca aaaagctgcc tttgacgagc tattgctata aataagagat
     5281 cttttttctt taattattta tttattttaa aatggttaag ttttgctaat catgatgagt
     5341 tatttttgat agttcatc
//
LOCUS       DAZPCS010000205         5340 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZPCS010000205 DAZPCS010000000
VERSION     DAZPCS010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5340
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.208"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(35..772)
                     /gene="bamD"
                     /locus_tag="ACHUQ7_003679"
     CDS             complement(35..772)
                     /gene="bamD"
                     /locus_tag="ACHUQ7_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFR1136894.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRIEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            907..1887
                     /gene="rluD"
                     /locus_tag="ACHUQ7_003680"
     CDS             907..1887
                     /gene="rluD"
                     /locus_tag="ACHUQ7_003680"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFR1136895.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1884..2615
                     /gene="yfiH"
                     /locus_tag="ACHUQ7_003681"
     CDS             1884..2615
                     /gene="yfiH"
                     /locus_tag="ACHUQ7_003681"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFR1136896.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2745..5318
                     /gene="clpB"
                     /locus_tag="ACHUQ7_003682"
     CDS             2745..5318
                     /gene="clpB"
                     /locus_tag="ACHUQ7_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFR1136897.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKATGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1310 a         1290 c         1458 g         1282 t
ORIGIN      
        1 ctcaagggct gccgttttgt gtttcaggtt tctgttatgt attgctgctg tttgcggcga
       61 tgattttcgc tactttttca gcttgcgcat tcatctgcat ctgacggtat gcattttcca
      121 tcagcggcag cgcatcacgc gtagcctggg tatccgggta gtcgcgcaac atgccttcta
      181 tgcggttaac gacggcaacc catgcgccac gttctgtata gtactcggcc acggagtatt
      241 catatttcgc cagacgatct ttcaggaata ccagacgttt ggtggcatcg gtagtgtact
      301 gactgttcgg atagccgcgc accagtttgg aaaagtcact aaacgcagct cgtgcatgtt
      361 gaggatcgcg atcgctacgg tcaacgccaa agaacccttg cagcgcactg tcatccagcg
      421 ccatattggt caggccacgc atgtacatga cataatcgat attcggatgg gtcgggttaa
      481 ggcgaataaa acgatcgatg gcagcctgcg ctaacggcaa atcggcgttt ttatagtagg
      541 cgtagatgag atccagctgc acctgctgcg aatacggacc aaacggatag cgattatcta
      601 acgcttccag ttgcgttatt gcctgtctcc agttaccgtc ctgcagcttt tgttgtgcag
      661 tcgcgtaaat ttcatttggc ggattatcag gtacttcttc ctttgacccc gagcaacccg
      721 ccaaaaacag gcttagtgtg gcggctgcca ccagatattt catgcgcgtc atgacgtttt
      781 gactttcctc aaaatgtaat acgggagatt ctctgttcct gcacccggtt aagaccagct
      841 acaatagcac actatattaa acggcaaagc cgtaaaaccc caacgataaa cgaagaagca
      901 gtatatatgg cacaacgagt acagctcact gcaacggtgt ccgaaaacca actcggtcaa
      961 cgcttagatc aggctttggc cgaaatgttc ccggattatt cacgttcgcg aataaaagaa
     1021 tggatcctcg accagcgcgt gctggtaaac ggcaaagttt gtgataagcc gaaagaaaaa
     1081 gtattgggtg gcgagcaggt tgccatcaac gctgagattg aagaagaagc gcgttttgaa
     1141 ccgcaggata tccctctgga tatcgtctat gaagatgaag atatcatcgt cattaataaa
     1201 ccgcgcgacc tagtggtaca tcctggcgcg ggtaacccgg atggcacggt actgaatgcg
     1261 ttgcttcatt actatccgcc cattgccgat gtaccacgtg cgggcatcgt ccatcgtctg
     1321 gataaagaca ccactggcct gatggttgtg gcaaaaaccg ttccggctca gacgcgttta
     1381 gtcgaatctt tgcaacggcg tgaaattact cgtgagtatg aagcggtggc gattggtcat
     1441 atgaccgctg gcggtacagt agatgaaccc atcagccgcc acccgaccaa acgtactcac
     1501 atggcggtgc atccgatggg caaaccggcg gtgactcact atcgcatcat ggaacacttc
     1561 cgtgtgcaca cgcgtctgcg gttgcgtctg gaaactggac gtacgcacca gatccgcgtg
     1621 catatggccc atatcactca tccgctggtg ggcgatccgg tttatggtgg ccgtccgcgt
     1681 ccgccaaaag gtgcttcgga agcatttatc tccacgctgc gtaagtttga ccgccaggcg
     1741 cttcatgcaa ccatgctgcg tctttatcac ccgatctccg gcatcgaaat ggaatggcat
     1801 gcgcctattc cacaagatat ggtggagctg attgaggtga tgcgcgccga tttcgaagag
     1861 cataaggatg aagtggactg gttatgagta agctgattgt cccgcagtgg ccgctgccaa
     1921 aaggtgttgc ggcctgtagc tccactcgta tcggcggcgt gagcttgccc ccgtatgact
     1981 cactcaacct cggtgcccat tgtggcgata acccggatca cgttgaggag aatcgcaagc
     2041 gactttttgc tgcgggcaat ttgccttcta aaccggtctg gcttgagcag gtacacggca
     2101 aagatgtgct taagcttact ggcgaacctt atgcctcaaa acgagcagat gcctcttata
     2161 gcaatacgcc tggcacggtt tgcgcagtga tgactgccga ctgcctccct gtgctgtttt
     2221 gcaatcgagc gggaacggaa gtcgccgccg ttcatgctgg ctggcgtgga ctgtgcgcag
     2281 gcgtgctgga agagacggtt tcctgttttg ctgataaacc tgaaaacatt ctcgcctggt
     2341 tagggccggc aattggtcca cgcgcgttcg aagtgggggc ggaggttcgc gaggcgttta
     2401 tggcagtaga cgctaaagca agtgcagctt tcattcagca tggtgataag tatctggcgg
     2461 atatttatca gcttgcccgg cagcgtctgg cgaacgtggg tgttgagcaa attttcggcg
     2521 gcgaccgttg tacatatacg gaaaatgaga ctttcttctc ttatcgtcgc gacaagacca
     2581 ccggtcgtat ggcaagtttc atttggctga tataacctaa agaatcaaga cgatccggta
     2641 cgcgtgattt tcttttcaca ttaatctggt caataacctt gaataattga gggatgacct
     2701 catttaatct ccagtagcaa ctttgatccg ttatgggagg agttatgcgt ctggatcgtc
     2761 ttactaataa attccagctt gctcttgccg atgcccaatc acttgcactc gggcacgaca
     2821 accaattcat cgaaccactt catttaatga gcgccctgct gaatcaggaa gggggttcgg
     2881 ttagtccttt attaacatcc gctggcataa atgctggcca gttgcgcaca gatatcaatc
     2941 aggcattaaa tcgtttaccg caggttgaag gtactggtgg tgatgtccag ccatcacagg
     3001 atctggtgcg cgttcttaat ctttgcgaca agctggcgca aaaacgtggt gataacttta
     3061 tctcgtcaga actgttcgtt ctggcggcac ttgagtctcg cggcacgctg gccgacatcc
     3121 tgaaagcaac aggggcgacc accgccaaca ttactcaagc gattgaacaa atgcgtggag
     3181 gtgaaagcgt gaacgatcaa ggtgctgaag accaacgtca ggctttgaaa aaatatacca
     3241 tcgaccttac cgaacgagcc gaacagggca aactcgatcc ggtgattggt cgtgatgaag
     3301 aaattcgccg taccattcag gtgctgcaac gtcgtactaa aaataacccg gtactgattg
     3361 gtgaacccgg cgtcggtaaa actgccatcg ttgaaggtct ggcgcagcgt attatcaacg
     3421 gcgaagtgcc ggaaggattg aaaggccgcc gcgtactggc gctggatatg ggcgcgctgg
     3481 tggctggggc gaaatatcgc ggtgagtttg aagaacgttt aaaaggcgtg cttaacgatc
     3541 ttgccaaaca ggaaggcaac gtcatcctgt ttatcgacga attacatacc atggtcggcg
     3601 cgggtaaagc cgatggcgca atggacgccg gaaacatgct gaaaccggcg ctggcgcgtg
     3661 gtgaattgca ctgcgtaggt gccacgacgc ttgacgaata tcgccagtac atcgaaaaag
     3721 atgctgcgct ggaacgtcgt ttccagaaag tgtttgttgc cgagccttct gttgaagata
     3781 ccattgcgat tctgcgtggc ctgaaagaac gttacgaatt gcaccaccat gtgcaaatta
     3841 ctgacccggc aattgttgca gcagcgacgt tgtctcatcg ctacattgct gaccgtcagc
     3901 tgccggataa agccatcgac ctgatcgatg aagcagcatc cagcattcgt atgcagattg
     3961 actcaaaacc agaagaactc gaccgactcg atcgtcgtat catccagctc aaactggaac
     4021 aacaggcgtt aatgaaagag tctgatgaag ccagtaaaaa acgcctggat atgctcaacg
     4081 aagaactgag cgacaaagaa cgtcagtact ccgagttaga agaagagtgg aaagcagaga
     4141 aggcatcgct ttctggtacg cagaccatta aagcggaact ggaacaggcg aaaatcgcta
     4201 ttgaacaggc tcgccgtgtg ggggacctgg cgcggatgtc tgaactgcaa tacggcaaaa
     4261 tcccggaact ggaaaagcaa ctggaagccg caacgcagct cgaaggcaaa actatgcgtc
     4321 tgttgcgtaa taaagtgacc gatgccgaaa ttgctgaagt gctggcgcgt tggacgggga
     4381 ttccggtttc tcgcatgatg gaaagcgagc gcgaaaaact gctgcgtatg gagcaagaac
     4441 tgcaccatcg cgtcattggt cagaacgaag cggttgatgc ggtatctaac gctattcgcc
     4501 gtagccgtgc ggggttggcg gatccaaatc gcccgattgg ttcattcctg ttcctcggcc
     4561 caacgggtgt ggggaaaact gagctttgta aggcgctggc gaactttatg tttgatagcg
     4621 acgaggcgat ggtccgtatc gatatgtccg aatttatgga gaaacactcg gtgtctcgtc
     4681 tggttggtgc gcctccggga tatgtcggtt atgaagaagg tggctacctg accgaagctg
     4741 tgcgtcgtcg tccgtattcc gtcatcctgc tggatgaagt ggaaaaagcg catccggatg
     4801 tcttcaatat tctgttgcag gtactggatg atgggcgtct gactgacggg caggggagaa
     4861 cggtcgactt ccgtaatacg gttgtcatta tgacctctaa cctcggttcc gatctgattc
     4921 aggaacgctt cggtgaactg gattatgcgc acatgaaaga gctggtgctc ggtgtggtta
     4981 gccataactt ccgtccggaa ttcattaacc gtattgatga agtggtggtc ttccatccgc
     5041 tgggtgaaca gcacattgcc tcgattgcgc agattcagtt gaaacgtctg tacaaacgtc
     5101 tggaagaacg tggttatgaa atccacattt ctgacgaggc gctgaaactg ctgagcgaga
     5161 acggttacga tccagtctat ggtgcacgtc ctttgaaacg tgctatccag cagcagatcg
     5221 aaaacccgct ggcacagcaa atactgtctg gtgaattggt tccgggtaaa gtgattcgcc
     5281 tggaagttaa tgaagaccgg attgtcgctg tccagtaatg ataaaacgag cccttcgggg
//
LOCUS       DAZPCS010000206         5338 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZPCS010000206 DAZPCS010000000
VERSION     DAZPCS010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5338
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.209"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            42..2078
                     /gene="yihQ"
                     /locus_tag="ACHUQ7_003683"
     CDS             42..2078
                     /gene="yihQ"
                     /locus_tag="ACHUQ7_003683"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HFR1136898.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHNKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRITSFGKRVMWNWKWNSENYPQLDSRIKQWYQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYSLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLNSI"
     gene            2124..3498
                     /locus_tag="ACHUQ7_003684"
                     /pseudo
     CDS             2124..3498
                     /locus_tag="ACHUQ7_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            3541..4944
                     /locus_tag="ACHUQ7_003685"
     CDS             3541..4944
                     /locus_tag="ACHUQ7_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1136899.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKLYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATTLFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEKKTASVTL"
     gene            5012..>5338
                     /locus_tag="ACHUQ7_003686"
     CDS             5012..>5338
                     /locus_tag="ACHUQ7_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligogalacturonate-specific porin KdgM family
                     protein"
                     /protein_id="HFR1136900.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSISS
                     GGAVYLD"
BASE COUNT         1254 a         1318 c         1347 g         1419 t
ORIGIN      
        1 ggaaggcaac aatctcgttc tcaactctat ggagattagt tatggatacg ccacgtccac
       61 agttattaga ttttcaattt catcagaata acgacagttt taccctacat tttcaacaac
      121 gtcttatttt aacccataac aaagataatc cttgtttatg gattggctca ggtatagcgg
      181 atatcgatat gttccgcggt aatttcagca ttaaagataa actacaggag aaaattgcgc
      241 ttaccgacgc catcgtcagc cagtcaccgg atggttggtt aattcatttc agccgtggtt
      301 ctgacattag cgccacgctg aatatctctg ccgacgatca gggccgttta ttgctggaac
      361 tacaaaacga caaccttaac cacaaccgta tctggctgcg ccttgccgct caaccagagg
      421 accatatcta cggctgcggc gaacagtttt cctacttcga tctgcgcggc aaaccgttcc
      481 cgctatggac cagtgaacaa ggcgttggtc gcaacaaaca aacctatgtc acctggcagg
      541 ccgactgcaa agaaaatgcg ggcggcgact attactggac tttcttccca cagcctacgt
      601 ttgtcagcac gcagaagtat tactgccatg ttgataacag ttgctacatg aacttcgact
      661 ttagcgcccc ggaataccat gaactggcgc tgtgggaaga caaagctacg ctacgttttg
      721 aatgtgctga cacatacatc tccctgctgg aaaaattaac tgccctgctg ggacgccagc
      781 cagaactgcc cgactggatt tatgacgggg taacgctcgg cattcagggc gggacggaag
      841 tgtgccagaa gaaactggac accatgcgta acgcgggcgt gaaggttaac ggcatctggg
      901 cgcaggactg gtctgggatc cgtataacct cttttggcaa gcgcgtgatg tggaactgga
      961 agtggaacag cgaaaactac ccgcaactgg attcacgcat taagcagtgg tatcaggagg
     1021 gcgtgcagtt cctggcctat atcaacccgt atgttgccag cgataaagat ctctgcgaag
     1081 aagcggcaca acacggctat ctagcaaaag atgcctctgg cggtgactat ctggtggagt
     1141 ttggcgagtt ttacggcggc gttgtcgatc tcactaatcc agaagcctac gcctggttca
     1201 aggaagtgat caaaaagaac atgattgaac tcggctgcgg cggctggatg gctgacttcg
     1261 gcgagtatct gcccaccgac acgtacctgc ataacggcgt cagtgccgaa attatgcata
     1321 acgcctggcc tgcgctgtgg gcgaagtgta actacgaagc ccttgaagaa acgggcaagc
     1381 tcggcgagat cctcttcttt atgcgcgccg gttctaccgg tagccagaaa tactccacca
     1441 tgatgtgggc gggcgaccag aacgtggact ggagtctcga cgatggcctg gcgtccgtag
     1501 tcccggcggc gctgtcgctg gcaatgaccg gacatggtct gcatcacagc gacattggcg
     1561 gttacaccac cctgtttgag atgaagcgca gcaaagagct gctgctgcgc tggtgcgatt
     1621 tcagcgcctt cacgccgatg atgcgcaccc acgaaggtaa ccgtcctggc gacaactggc
     1681 agtttgacgg cgatgcggaa actatcgccc attttgcccg tatgactacc gttttcacca
     1741 ccctgaaacc ttatctgaaa gaggccgtcg cgctgaatgc gaagtccggt ctgccggtca
     1801 tgcgcccgct gttcctgcat tacgaagacg atgcacatac ctattccctg aaatatcagt
     1861 acctgttagg ccgcgacatt ctggtcgctc cggtacacga agaaggtcgc agcgactgga
     1921 cgctctatct gccggaggat aactgggtac atgcctggac gggtgaagcg ttccggggcg
     1981 gggaagttac cgttaatgcg cccatcggca agccgccggt cttttatcgc gccgatagcg
     2041 aatgggcggc actgttcgcg tcgttaaaca gcatctaatc acatccgccc gtggggaaac
     2101 tcatgggcaa agggagaaca tcgatgagtc acatcacaac ggaagatcca gcaactttac
     2161 gcctgccctt taaagagaaa ctctcttacg gtattggcga cctggcctct aacatcctgc
     2221 tggatatcgg tacgctttat cttttgaagt tttataccga cgttctgggg ctgccaggca
     2281 cctatggcgg cattatcttt ttgatttcaa aattctttac tgcgtttacc gatatgggta
     2341 ccggcattat gttggattcc cgacgcaaga tcggtccaaa aggtaagttc cgtcctttta
     2401 ttctgtatgc gtcattcccg gtcaccttac tggcgatcgc caactttgtc ggcacaccgt
     2461 ttgatgtcac cggtaaaacg gtgatggcca ctattctgtt tatgctttac ggactgtttt
     2521 tcagcatgat gaactgctcc tacggcgcaa tggtcccggc aatcaccaaa aaccccaacg
     2581 aacgcgcctc actggcggca tggcgtcagg gcggcgctac gctgggcctg ctgctgtgca
     2641 cggtgggatt cgtgccagtt atgaatctta tcgaaggtaa tcagaaactt ggctatatct
     2701 tcgccgccac gctgttttca ctgttcggcc tgctgtttat gtggatctgc tactcaggcg
     2761 tgaaagagcg ttatgtcgaa acccaaccaa ccaatccggc gcaaaagcct ggcctgttgc
     2821 aatctttccg cgcaattgcc ggtaaccgcc cactgttcat tctgtgtatt gccaacctct
     2881 gcaccttagg ggcgtttaac gtcaagctcg ccattcaggt ctattacacc cagtacgtac
     2941 ttaacgatcc catcctgttg tcgtatatgg gatttttcag catgggctgt attttcatcg
     3001 gcgtgttcct gatgcctggc gcagtcagac gttttggtaa gaagaaggtc tatatcggcg
     3061 gcctgctgat ttgggtgctg ggcgatctgc tcaactattt cttcggcggc ggctcggtca
     3121 gctttgtggc gttctcctgc ctggcgttct tcggctcagc gcctgaactg ggcgctggtt
     3181 tccgacaccg tcgagtacgg cgagtggcgc accggcgtgc gttcggaagg cacggtctac
     3241 accggcttca ccttctttcg caaagtgtct caggcgctgg ctggtttctt ccccggctgg
     3301 atgctgacgc aaattggcta tgtgccgaac gtcgcacagg ctgaccacac cattgaaggg
     3361 ttacgccagt tgatcttcat ctacccaagc gcactggcgg tagtcaccat tgtggcgatg
     3421 ggttgcttct acagcctgaa cgagaagatg tatgtccgca ttgtggaaga gatagaagcc
     3481 cgtaaacgca cggcgtaata ataataaacg acgccctgcg gggcgttata aggagtgatt
     3541 atgtctgacc ataatccact gacattaaaa ctgaatctgc gggaaaaaat cgcctatggt
     3601 atgggcgacg tcggttcgaa tttaatgctc tgcatcggta ctctgtatct cctcaaactt
     3661 tataccgatg aattagggat gcctgcttac tatggcggga ttatctttct ggtcgcgaag
     3721 ttttttaccg cgtttaccga tatgctcacc ggatttttac tcgactcgcg gaaaaatatt
     3781 gggcctaaag gcaaatttcg tccctttatt ttatatgcag cagttccggc ggcgttaatt
     3841 gcaacgctcc agtttatcgc caccaccttt tgtttgccgg ttaaaacgac gattgccacc
     3901 acgctgttta tgatgtttgg actttcatac agtctgatga actgctcgta tggtgcgatg
     3961 atcccggcaa ttaccaaaaa cccgaatgag cgcgcgcagc tcgcggctta ccgccagggt
     4021 ggtgcaacca tagggctatt gatttgtacc gtggcgttta ttccgttgca gtcgcttttt
     4081 tctgactcaa ccgtcggtta tgcctgtgcg gcacttatgt tctccattgg cggctttatt
     4141 tttatgatgc tgtgctacag aggcgtcaaa gagcattatg tggacacagc gccaaccgga
     4201 cataaagcca gtattctcaa atctttttgc gcgatatttc gtaatccacc attgctggtt
     4261 ttatgcattg ctaacctgtg tactctggcg gcatttaata tcaaactggc gattcaggtc
     4321 tattacaccc agtatgtgct gaatgatatt aatttattgt cgtggatggg attcttcagt
     4381 atgggatgca tcctcgtcgg cgtattactg gtgccagtaa ctgtaaaatg ttttggtaaa
     4441 aaacaggttt atttagctgg catggtgcta tgggcggtgg gtgatatact gaattatttc
     4501 tggggaagta actctttcac tttcgtcatg ttctcttgtg tcgccttttt tggcacggcc
     4561 tttgtaaaca gcctgaactg ggcactggta ccagataccg tagattacgg tgaatggaaa
     4621 accggtattc gtgccgaagg ttctgtttat accggttata ccttctttcg taaaatttct
     4681 gccgcacttg ctggcttctt gccaggcatt atgctgacgc aaattggtta tgttcccaac
     4741 atcgcgcaaa gcgatgcgac attacagggt ttgcgtcagc tcatttttat ctggccttgt
     4801 gcactggcaa ttattgctgc attgacgatg ggattctttt acacactcaa tgaaaaacgg
     4861 tttgcattga ttattgagga aatcaaccaa cgcaaaaata aagagatggc gacagaaaaa
     4921 aaaacggctt ccgtaacgtt ataaatgtca atagccgcta tttccatcct ggtgggtggc
     4981 ggcctcccta cgtttaaaaa atggacttat tatgaaaaag attaatgcaa taattttatt
     5041 atcttctctg acctctgcct cggtatttgc tggcgcatac gtcgaaaaca gggaggcata
     5101 taatcttgcc tccgatcagg gcgaggttat gctacgtgtg ggttataact tcgatatggg
     5161 cgcgggtatt atgttaacca atacctacac ctttcagcga gaagatgaac taaaacatgg
     5221 ctataatgaa attgaaggtt ggtatccttt attcaaacca accgataaat taaccatcca
     5281 gcccggtggc ttaattaatg ataagagtat cagttcgggt ggtgcagtgt atctggac
//
LOCUS       DAZPCS010000207         5281 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZPCS010000207 DAZPCS010000000
VERSION     DAZPCS010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5281
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.210"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(67..1827)
                     /gene="ycaO"
                     /locus_tag="ACHUQ7_003687"
     CDS             complement(67..1827)
                     /gene="ycaO"
                     /locus_tag="ACHUQ7_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFR1136901.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            1957..2583
                     /locus_tag="ACHUQ7_003688"
                     /pseudo
     CDS             1957..2583
                     /locus_tag="ACHUQ7_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            2782..3870
                     /gene="serC"
                     /locus_tag="ACHUQ7_003689"
     CDS             2782..3870
                     /gene="serC"
                     /locus_tag="ACHUQ7_003689"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFR1136902.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            3941..5224
                     /gene="aroA"
                     /locus_tag="ACHUQ7_003690"
     CDS             3941..5224
                     /gene="aroA"
                     /locus_tag="ACHUQ7_003690"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFR1136903.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
BASE COUNT         1313 a         1263 c         1358 g         1347 t
ORIGIN      
        1 taaaaaaacg ccccgtaata taactcagac taatcattaa gcctacattg cgtaggctat
       61 ttgattttat tttgcccaga atgctgcttt ggcgcgctgc agcttttcgt aggccttcaa
      121 caacgactga tgtgcagcaa acgcgtgcag atcgctatct accggttgca ggccgtaaaa
      181 cgccgcttcg ccgctcattg ccgcactggc ggcttctacg gcatctgcgc cgtacatgcg
      241 aacaaacgca ttcagatatt gcagcggctg gcgatcttct tcctgtgcca gtaataacaa
      301 cgtttgcagg cagcgataat agttggcgcg ttccggacta aatactgatg agttaaactc
      361 catcgtccat tcggtccaga ccagagcctg ttccagatcg ccaccagcca gcgccagcat
      421 ggcttttaat tcaccgatac gcagggtgta ccaaccgtta tccgacccgg tcgccagacc
      481 caacagctca cgcacgcggg taaagtcatc aaaaccttct tcatccagtt gctcgatgag
      541 gttcaggtaa tcttcttttt cccactcgct gcctggtagc gaaagaatcg tttcacgtaa
      601 atggctgccc atactgttat tcgcgagcca cagatcttca gccggataaa tatcggacat
      661 gccaggcacg ataatacggc aagcataaac gcccagatgc tcgtaatcgg caatataaac
      721 ttctttatct tctttgttga agatagccat cagcgtagca aactcttctt ccgtggtgcc
      781 ggagaaattc cagtccacaa acggataatc ggcatcctgc ttgaacaggt cccaggagat
      841 taaaccgctg gaatcgataa agtgcgtttc gaggttggta tgttcagcga cttcttcatc
      901 atcgaaggtt ggcggagtaa acacatccaa atctttcagg ccacgacctt gcagcagctc
      961 ggtcacggta cgttccagtg ctacgccaaa atcaggatgc gcaccgaaag aggcaaagca
     1021 ggtgccgttt gcaggattaa acagtaccac gcaaatcacc ggatactggc cgccaaggga
     1081 accatcatat gcgaagatcg ggaaaccttc tgtttccagt gtttcgatcg cttcaaccac
     1141 cgctgggtaa cgcgccagca cgtctgccgg gatttccggc aagctgatgc tttcagcaat
     1201 aatgcggttt ttcacgtagc gttcgaaaac ttcggacaac ccctgaacgc gtgcttcgtt
     1261 gcgggtattg ccagcggaca taccgttaga aacgtacagg ttaccaatga tattcatcgg
     1321 aatataaacg gtctgattgt cggactggcg cgtaaacggc agaccgcaaa taccacgatc
     1381 ttcgttaccg gattgtaggt caatcagcat actgccggtc aattcattct ccggatcgta
     1441 aaatgcgcgc agacggtcat cgagcagccc ttctggcaca tcgtcatttt cgctcagtgg
     1501 gaaccatttt tcgttgggat aatgcacaaa tggaccgttg gcgatggttt cgcccagcca
     1561 gaaatcagca aaaaagtagt tggttgagag acgctcgaaa tattcaccga gtgcagaagc
     1621 cagcgccgct ttcttggttg cgcctttacc gttggtaaaa cacagtgcgc actctttgtc
     1681 gcgaatatgt acagaccaga cgttaggcac gggattcagc caggaggcct cttcaatctg
     1741 aaagccgagg tctgaaagtt tttgctggaa gcgagcgatg gaatcttcca gagcggcatc
     1801 tttgccgggg ataaatgttt gcgtcatgaa aatcacttta gtcgtacgga aagcgcgcaa
     1861 taatacgggt tttatctcaa aggcgctatc accgccgcca tgccgatgaa ctgttgacta
     1921 tgcttttagc ggataaacca cgttaagagc ataaaaatga aggcgttcga tctccaccgt
     1981 atggcatttg ataaagtgcc ttttgatttc cttggcgaag ttgcactacg tagtctttat
     2041 acctttgtac tggttttttg ttcctcaaaa tgaccggaag acgcggtgtg cgacagatgt
     2101 cgctgtttga agtgttaatc attctcacgc tgggttcagc ggcgggagat gtggcgtttt
     2161 atgatgatgt gccgatggtg ccggtactta tcgtctttat cactctggcg ttgttatacc
     2221 gcctggtaat gtgatggcga gctggcctgg tcgaaactca ataactccaa catgacggaa
     2281 tttgagttct ttatggagct acgattgcgt ggcgtggagc agctggggca ggtacgtctg
     2341 gcgattctcg aaaccaacgg gcaaatcagt gtctatttct ttgaagatga caaggtgaaa
     2401 ccgggtttac ttattttacc cagtgattgt acgcagcgtt acaaagtggt gccggagtcg
     2461 gcggactatg cctgcattcg ttgtagtgaa atcattcata tgaacgcggg ggaaaaacaa
     2521 ttatgtccgc gctgtgcaaa tccagaatgg acgaaggcaa gtcgggcaaa acgggtgacc
     2581 tgacagtaaa aacatcggct ttttgctaat aatccgagag attcttttgt gtgatgcaag
     2641 ccacattttt gccctcaacg gttttactca ttgcgatgtg tgtcactgaa tgataaaacc
     2701 gatagccaca ggaataatgt attacctgtg gtcgcaatcg attgaccgcg ggttaatagc
     2761 aacgcaacgt ggtgagggga aatggctcaa atcttcaatt ttagttctgg tccggcaatg
     2821 ctaccggcag aggtgcttaa acaggctcaa caggaactgc gcgactggaa cggtcttggt
     2881 acgtcggtga tggaagtgag tcaccgtggc aaagagttca ttcaggttgc agaggaagcc
     2941 gagaaggatt ttcgcgatct tcttaatgtc ccctccaact acaaggtatt attctgccat
     3001 ggcggtggtc gcggtcagtt tgctgcggta ccgctgaata ttctcggtga taaaaccacc
     3061 gcagattatg ttgatgccgg ttactgggcg gcaagtgcca ttaaagaagc gaaaaaatac
     3121 tgcacgccta atgtctttga cgccaaagtg actgttgatg gtctgcgcgc ggttaagcca
     3181 atgcgtgaat ggcaactctc tgataatgcg gcttatatgc attactgccc gaatgaaacc
     3241 atcgacggta tcgccatcga cgagacgcca gacttcggca aagatgtggt ggtcgccgcc
     3301 gacttctctt caaccattct ttcccgtccg attgacgtca gccgttatgg tgtaatttac
     3361 gctggcgcgc agaaaaatat cggcccggct ggcctgacaa tcgtcatcgt tcgtgaagat
     3421 ttgctgggca aagcgaatat cgcgtgtccg tcgattctgg attattccat cctcaacgat
     3481 aacggctcca tgtttaacac gccgccgaca tttgcctggt atctatctgg tctggtcttt
     3541 aaatggctga aagcgaacgg cggtgtagct gaaatggata aaatcaatca gcaaaaagca
     3601 gaactgctat atggggtgat tgataacagc gatttctacc gcaatgacgt ggcgaaagct
     3661 aaccgttcgc ggatgaacgt gccgttccag ttggcggaca gtgcgcttga caaattgttc
     3721 cttgaagagt cttttgctgc aggccttcat gcactgaaag gacaccgtgt ggtcggcgga
     3781 atgcgcgcgt ctatttataa tgccatgccg ctggaaggcg ttaaagcgct gacagacttc
     3841 atggttgagt tcgaacgccg tcacggttaa tgccgaaatt ttgcttaatc cccacagcca
     3901 gcctgtgggg tttttatttc tgttgtagag agttgagttc atggaatccc tgacgttaca
     3961 acccatcgct cgtgtcgatg gcactattaa tctgcccggt tccaagagcg tttctaaccg
     4021 cgctttattg cttgcggcat tagcacacgg caaaacagta ttaaccaatc tgctggatag
     4081 cgacgacgtg cgccatatgc tgaatgcatt aacagcgtta gggttaagct atacgctttc
     4141 agccgatcgt acgcgttgcg aaattatcgg taacggcggt ccattacacg cagaaggtgc
     4201 cctggagttg ttcctcggta acgccggaac ggcaatgcgt ccgctggcgg cagctctttg
     4261 tctggatagc aatgatattg tgctgaccgg tgagccgcgt atgaaagagc gcccgattgg
     4321 tcatctggtg gatgcgctgc gcctgggcgg ggcgaagatc acttacctgg aacaagaaaa
     4381 ttatccgccg ttgcgtttac agggcggctt tactggcggc aacgttgacg ttgatggctc
     4441 cgtttccagc caattcctca ccgcactgtt aatgactgcg cctcttgcgc cggaagatac
     4501 ggtgattcgt attaaaggcg atctggtttc taaaccttat atcgacatca cactcaatct
     4561 gatgaagacg tttggtgttg aaattgaaaa tcagcactat caacaatttg tcgtaaaagg
     4621 cgggcagtct tatcagtctc cgggtactta tttggtcgaa ggcgatgcat cttcggcttc
     4681 ttacttcctg gcagcagcag caatcaaagg cggcactgta aaagtgaccg gtattggacg
     4741 taacagtatg cagggtgata ttcgctttgc tgatgtgctg gaaaaaatgg gcgcgaccat
     4801 ttgctggggc gatgattata tttcctgcac gcgtggtgaa ctgaacgcta ttgatatgga
     4861 tatgaaccat attcctgatg cggcgatgac cattgccacg gcggcgttat ttgcaaaagg
     4921 caccaccacg ctgcgcaata tctataactg gcgtgttaaa gagaccgatc gcctgtttgc
     4981 gatggcaaca gaactgcgta aagtcggcgc ggaagtggaa gaggggcacg attacattcg
     5041 tatcactcct ccggaaaaac tgaactttgc cgagatcgcg acatacaatg atcaccggat
     5101 ggcgatgtgt ttctcgctgg tggcgttgtc agatacacca gtgacgattc ttgatcccaa
     5161 atgcacggcc aaaacatttc cggattattt cgagcagctg gcgcggatta gccaggcagc
     5221 ctgaatgaac aacgggcaat aaatagccaa atctttcttt atcaaaacgt cggcacattg
     5281 t
//
LOCUS       DAZPCS010000208         5164 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZPCS010000208 DAZPCS010000000
VERSION     DAZPCS010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5164
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.211"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..244
                     /locus_tag="ACHUQ7_003691"
     CDS             <1..244
                     /locus_tag="ACHUQ7_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1136904.1"
                     /translation="EMLSFLTSEEREALTNMLARKGVETVLRLLDKETQELLQLEGRK
                     RLAALMLVDMPDEQVREILEEIEASILSESVNAKAG"
     gene            493..1437
                     /gene="tauA"
                     /locus_tag="ACHUQ7_003692"
                     /pseudo
     CDS             493..1437
                     /gene="tauA"
                     /locus_tag="ACHUQ7_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
     gene            1450..2217
                     /gene="tauB"
                     /locus_tag="ACHUQ7_003693"
     CDS             1450..2217
                     /gene="tauB"
                     /locus_tag="ACHUQ7_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFR1136905.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLRLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            2214..3041
                     /gene="tauC"
                     /locus_tag="ACHUQ7_003694"
     CDS             2214..3041
                     /gene="tauC"
                     /locus_tag="ACHUQ7_003694"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFR1136906.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            3038..3889
                     /gene="tauD"
                     /locus_tag="ACHUQ7_003695"
     CDS             3038..3889
                     /gene="tauD"
                     /locus_tag="ACHUQ7_003695"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFR1136907.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSWLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(3995..4969)
                     /gene="hemB"
                     /locus_tag="ACHUQ7_003696"
     CDS             complement(3995..4969)
                     /gene="hemB"
                     /locus_tag="ACHUQ7_003696"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFR1136908.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
BASE COUNT         1181 a         1304 c         1469 g         1210 t
ORIGIN      
        1 agaaatgttg tcgtttctta ccagtgagga acgagaagcc cttacaaaca tgctcgcaag
       61 gaaaggtgtt gagactgtgc tacgcttgtt agataaggaa actcaagagc tactgcaact
      121 agagggccgt aaacgcctgg cagcgttgat gctcgttgat atgcccgacg agcaagtcag
      181 agagattttg gaagagattg aagctagtat tctctcagag agtgtaaacg cgaaggcggg
      241 ttaacttcat tgacctgcct ttagggtgcc tttaaaattc gtttttaaag gctacgatga
      301 catttttgaa gagtcttaaa agaattaaaa tacacggcgc gcctatcttg aatgttttat
      361 cgatatcgaa gcattgagca tttttaactc gttttatctt gaatgcaatc ggcgcataac
      421 ttattgaatc ccatcaaatc ccgtcttttt ttacccattc ccggttatct tgaataaatt
      481 acctagtcat ataacacact tcttgccgca ctggcattca tcgcttttca ggcacaggcg
      541 gtgaacgtca ccgtggcgta tcaaacctca gccgaaccgg cgaaagtggc tcaggccgac
      601 aacacctttg ctaaagagag cggggcgagc gtggactggc gtaagtttga cagcggagcc
      661 agcatcgtgc gggcgctggc ttctggcgac gtgcaaatcg gcaacctcgg ttccagccca
      721 ttagcggttg cagccagcca acaggtgccg attgaagtct tcttgctggc gtcaaaactg
      781 ggtaactccg aagcgctggt ggtaaagaaa actatcagca aacccgaaga tttgattggt
      841 aaacgcattg ccgtaccgtt tatctccacc acccactaca gcctgctggc ggcgctgaaa
      901 cactggggga ttaaaccagg gctagtggag attgtgaacc tgcagccgcc cgcgattatc
      961 gccgcatggc agcggggtga tattgatggt gcttatgtct gggcaccggc ggttaacgcc
     1021 ctggaaaaag acggcaaggt gctgaccgat tctgaacagg tcgggcagtg gggtgcgcca
     1081 acgctggacg tctgggtggt gcgcaaagat tttgccgaga aacatcctga ggtcgtgaaa
     1141 gcgttcgcca aaagcgccat cgatgcacag caaccgtaca ttgctaaccc tgacgcgtgg
     1201 ctgaaacagc cggaaaacat cagcaaactg gcgcgtttaa gcggcgtgcc tgaaggtgac
     1261 gttccggggc tggtgaaggg aaatacctat ctgacgccgc agcaacaaac ggcagaactg
     1321 accggaccgg tgaacaaagc aatcatcgac accgcgcagt ttttgaaaga gcagggcaag
     1381 gtcccggctg tagcgaatga ttacagccag tacgttacct cgcgcttcgt gcaataaaag
     1441 gaggcgcaga tgctgcaaat ctctcatctc tatgccgatt atggcggcaa accggcactg
     1501 gaagatatca acctgacgct ggaaagcggc gagctactgg tggtgctggg gccgtccggc
     1561 tgcggtaaaa ccaccctgct gaatctgatt gccggttttg tgccttatca gcatggcagc
     1621 attcaactgg cgggtaagcg tattgaggga ccgggagcag agcgtggcgt agtttttcag
     1681 aatgaagggc tgctaccgtg gcgcaatgta caggacaacg tggcgttcgg cctgcaattg
     1741 gcaggtatag agaaaatgca gcgactggaa atcgcgcacc agatgctgaa aaaagtgggg
     1801 ctggaaggcg cagaaaaacg ctatatctgg cagctttccg atggtcaacg tcagcgggtg
     1861 gggattgccc gcgcgctggc ggcgaatccc cagctgttat tactcgacga accgtttggt
     1921 gcgctggacg ccttcacccg cgaccagatg caaactctgc tgctgaaact ctggcaggag
     1981 acgggcaagc aggtgctgtt gattacccac gatatagaag aagcggtgtt tatggcgact
     2041 gaactggttc tgctttcatc cggccctggc cgtgtgctgg agcggctgcg gctcaacttt
     2101 gctcgccgct ttgttgcggg agagtcgagc cgcagcatca agtccgatcc acaattcatc
     2161 gccatgcgcg aatatgtttt aagccgcgta tttgagcaac gggaggcgtt ctcatgagtg
     2221 tgctcattaa tgaaaaactg cattcgcggc ggctgaaatg gcgctggccg ctctcgcgtc
     2281 aggtgacctt aagcattggc acgttagcgg ttttactcac tgtatggtgg acggtggcgg
     2341 cgctgcaact gattagcccg ctgtttttgc cgccgccgca acaagtactg gcaaaactgc
     2401 tcaccattgc cggaccgcag ggatttatgg acgccacgct gtggcagcat ctggcagcca
     2461 gcctgacgcg cattgtgctg gcgctactcg cggcggtggt gatcggtatt ccggtcggga
     2521 tcgccatggg gctaagcccg acggtgcgcg gcattctgga tccggtaatc gaactttatc
     2581 gtccggtgcc accgctggct tatttgccgc tgatggtgat ctggtttggt attggtgaaa
     2641 cctcgaagat cttactgatc tatttagcga tttttgcgcc ggtggcgatg tcggcgctgg
     2701 caggggtgaa aagcgcgcag caggttcgca ttcgtgccgc ccagtcgctg ggtgccagcc
     2761 gtgcgcaggt gctgtggttt gtcattttgc ccggcgcgct gccggaaatc ctcaccggat
     2821 tacgcattgg tctgggtgtg ggctggtcta cgctggtagc ggcggagctg attgccgcga
     2881 cacgcggttt aggatttatg gttcagtcag cgggtgaatt tctcgcgact gacgtggtac
     2941 tggcggggat cgcggtgatt gcgattatcg cctttctttt agaactgggt ctgcgcgcgt
     3001 tacagcgccg tctgacgccc tggcatggag aagtacaatg agtgaacgtc tgagcattac
     3061 cccgctgggg ccgtatatcg gcgcacaaat ttcgggtgcc gacctgacgc gcccgttaag
     3121 cgataatcag tttgaacagc tttaccatgc ggtgctgcgc catcaggtgg tatttctgcg
     3181 cgatcaagct attacgccgc agcagcaacg cgcgctggcc cagcgttttg gcgaactgca
     3241 tattcaccct gtttaccctc atgccgaagg ggttgacgag atcatcgtgc tggataccca
     3301 taacgataat ccgccggata acgacaactg gcataccgat gtgacattta ttgaaacgcc
     3361 acccgcaggg gcgattctgg cagctaaaga gttaccttcg accggcggtg atacgctctg
     3421 gaccagcggt attgcggcct atgaggcgct ctctgttccc ttccgccagc tgctgagctg
     3481 gctgcgtgcg gagcatgatt tccgtaaatc attcccggaa tacaaatacc gcaaaaccga
     3541 ggaggaacat caacgctggc gcgaggcggt cgcgaaaaat cctccgttgc tacatccggt
     3601 ggtgcgaacg catccggtga gcggtaaaca ggcgctgttt gtgaatgaag gctttactac
     3661 gcgaattgtc gatgtcagcg agaaagagag cgaagccttg ttaggctttt tattcgccca
     3721 tatcactaaa ccggagtttc aggtgcgttg gcgctggcaa ccgaatgata ttgcgatttg
     3781 ggataaccgc gtgactcagc actatgccaa tgccgattac ctgccacagc ggcggattat
     3841 gcatcgggcg acgatccttg gggataaacc gttttatcgg gcggggtgat acgagtgcgg
     3901 acggttccct cgcccccttg gggagagggt tagggtgagg gggcgttcac tgtactttca
     3961 acaggttaac tccccctttc tgagaggaaa aaaattaacg cagaatcttc ttctcagcca
     4021 aatccagcgc aaagtagctg aaaatcagat ccgcacccgc acgcttaatt gaacctaagc
     4081 tttcgagcac gactttctct tcatctatag cacccgccag cgcggcgaac ttaatcatcg
     4141 cgtactcacc gctcacctga tacgcgccaa tcggcaattc agtacgttca cgcagctcac
     4201 gcacgatgtc gaggtacgct ccagcaggtt taaccatcag gcagtctgcg ccctgggctt
     4261 catccagcag tgactcacga atcgcctcgc gacggttcat tgggttcatc tgatagcttt
     4321 tgcggtcgcc ttttaatgcg cttcctgcag cttcacggaa cgggccataa aaggaagagg
     4381 cgaacttggt cgaatacgac ataatcgccg tatctttaaa gcctgcagcg tccagcgcct
     4441 gacgaatcgc ctgtacctgg ccgtccatcg cggcggaagg ggcgatgaag tctgcgcctg
     4501 cagcagctgc aaccacggct tgcttgccta aattttccag agtcgcgtcg ttgtcgacgc
     4561 catgctcgca cagcacaccg cagtgaccgt gagaagtgta ttcacagaaa caagtgtctg
     4621 acatgacgat catttctggc acagtctgct tgcagatgcg cgacatacgc gccaccagtc
     4681 catcttcccg ccaggcgtcg ctgccggttt catcggtatg gtgagagatg ccaaaagtca
     4741 ttacggaacg gataccggcg ttggcgatgc gttcaatttc gcgtgccaga tgtttctctg
     4801 gaatgcgcat cacgcctggc atggcttcaa cggctttgta gtcgtcaatt tcttcttcaa
     4861 caaagatcgg caacaccagg tcgttaaggc taagtgttgt ctcttcaaac atagcgcgca
     4921 gcgcaggaga tttgcgcagg cgacgagggc gttggattaa gtctgtcatg gtctgcctga
     4981 tgtttgtgga attaaggtgc acagtatacc tgaagcgggg caggagtgtt ttacgaaagt
     5041 tgtcgcgatg ttgacaagaa gagaatgaaa gagagaccag gacatctttg taatagaaat
     5101 agcaataatt tcatattcac tgaaaatatt tttaatcttt atttatagct cttggttatt
     5161 attt
//
LOCUS       DAZPCS010000209         5163 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZPCS010000209 DAZPCS010000000
VERSION     DAZPCS010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5163
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.212"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..220
                     /locus_tag="ACHUQ7_003697"
     CDS             <1..220
                     /locus_tag="ACHUQ7_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1136909.1"
                     /translation="LYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHW
                     GFSDQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(392..721)
                     /locus_tag="ACHUQ7_003698"
     CDS             complement(392..721)
                     /locus_tag="ACHUQ7_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFR1136910.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINAERKASYQQRAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(726..911)
                     /locus_tag="ACHUQ7_003699"
     CDS             complement(726..911)
                     /locus_tag="ACHUQ7_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFR1136911.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(908..3547)
                     /locus_tag="ACHUQ7_003700"
     CDS             complement(908..3547)
                     /locus_tag="ACHUQ7_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFR1136912.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLADIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWLYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLNVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRNPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGLMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3755..4744
                     /gene="ldhA"
                     /locus_tag="ACHUQ7_003701"
     CDS             3755..4744
                     /gene="ldhA"
                     /locus_tag="ACHUQ7_003701"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFR1136913.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSHPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4855..>5163
                     /gene="hslJ"
                     /locus_tag="ACHUQ7_003702"
     CDS             4855..>5163
                     /gene="hslJ"
                     /locus_tag="ACHUQ7_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFR1136914.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     N"
BASE COUNT         1307 a         1323 c         1203 g         1330 t
ORIGIN      
        1 tttgtaccga atgtttgccg ataaaggttt ggtagtcgcg caatatattc gtaaccgtcg
       61 tctcgatttt tgtgcagatg cgattcgcca tgccgcagat gatgaaaaac tggcaggcat
      121 cggctttcat tggggatttt ctgaccagag tcatttttca acggtattta agcaacgctt
      181 tgggatgacg ccaggcgagt atcgacgtaa attccgctaa ctaattggcg ttgcatgtac
      241 tgcaacgcca attagttaaa acagatattt gactatgcat ttttcagggc gaaaggcaat
      301 ttgacaggag tttttgaata ttcaccttgt ttcaggaaaa tagatcaatc gctttctcct
      361 gtagcgttat cccgatattg cggttttgct attaaaactt tcgcacccat ttaccgttta
      421 tcccttgtac atattccccc ggtttggccc gagctacgag cttttgcccg gcgagtttcg
      481 cgatatcatc tacagacaca ttattttgct ttgcccgttg ttggtaactc gctttacgtt
      541 cggcattaat ttcggttact aatttctctg tttcagcatc cgttttcagc gcaaccagat
      601 aaccataaaa tgtttcacct acccgcccct gagttctggc ttcatccaga gtcaatgcca
      661 ttacattgct gcttaccagc ccaacaagaa acgcacaaag aagtaatgtt ttcttcatca
      721 tcgcctcaga aaagatcgct acgagtttca agcagctctt cgacatcttt gtctgccttg
      781 atgatgatct catgctcaat tttaacgttc atattgatag tgatcggttc cttaggtgca
      841 gcgacttcaa tgcgaggcgt acagccaacc agcataaaag atgacgtcaa cgcagccagt
      901 aaaattttca ttgtttttcc tcacactctt ttccttgcgg acagtcattt cccggcagac
      961 gtgcgttctg ctccaaccat gcctggagat tatcgccaaa gcgtaaactg cgccacagcg
     1021 taaaaatatt ctcttcatga tggtaattaa ggtttaccgt accgctttta ccatcaacgc
     1081 gactggtacc tgtaatgttg gcctgcatgg ttaataaacc gagattatct aagttaattt
     1141 ttgtcgatga ccggctaatt tccatatagc gcaaccagtt aattgctgaa cccgcagtca
     1201 tattgtcttt caccaccgca tctgcggtat ctttatccag ccgcaaggtc attagcccgc
     1261 tatttgccag ccaaccatct ttcactatcc atttttcgtt gtttagccat aacggcaatg
     1321 caccactaaa tgccccggac atggcgaatt gtttcggatt gacggcgcta accagttcgc
     1381 tggatgagag attattcagc cgcaacagcg ccgggtcatg ttgcggcata cgtaattgtt
     1441 tcatcagcac gttaccgcct aacacatcga cgctaacatc agtcaacagc aagggttctt
     1501 cttcagtcca cggataacgc ccttgcaaat cagccgtaat atttttcgct gtcaccagat
     1561 taatcacttc ggcaattcgc aacgtaacgg ggccgcgagt ccccagatgc caggctccat
     1621 cggcaaaacg gaaaggcagg acaaaatcga caccgttaac ctgattatct ggcatccagg
     1681 cactaccgcc tttcaacacg ccgtgccctc ccgcgcggaa tccttgttca ggcgcagcag
     1741 aaaatgcaac ctgagcatac agttcaccat cgcgtaagtt catcttccag tcgggtggca
     1801 ccagcggctg gaatacagtc agtgattgtt ttggccacca ggcgttaccg cgcagacgaa
     1861 taccgtccca gcgaccattt acccgaaccg ggccaatctc accagcatgt aaatcgcctt
     1921 taaagaggaa ataggtcgga ttgcgcccat cgacgctaaa ttttaaggtt gatggcggta
     1981 acacactgcc gccagtgaac agcgtttgcc cggcgtccag tgacagcgcg ccgctgaagg
     2041 agggatgctg tgcgtcacgt acccagacga cgggcttgtc gagaattaat cgcggctttt
     2101 ctaccgtcat cgtaccgtat tgtaactggt cgaaaccggt ggagagatcg gtcagcgtaa
     2161 tcgtgctgtc atgccactca ccttttcctg cgacatccca ggtggcattc atcggtgtaa
     2221 aactcccttt tccccagtag cgccactgcc agcggccagc gtcagggaga aaatcattcg
     2281 ccagcccatc catatgcagc acgaaatcgc ccagttcatt ttcatgcgcc tgcaaaatgg
     2341 cctgcaaacg tccgtcaaca ccacgttggg tgacttttac acccgctaaa ggccagcgga
     2401 tttcatcgat gtccagcgaa tcgatgactc ttccctttga acgaagtaac gcgcctggct
     2461 caaaggtcag cgttgggtca gacagacttc cacttagctg cgcaggtaaa cgggcatata
     2521 aaatgaggtc cgcctgtttc gcttcaccgg tcagctgcag aggcagttga ctgttatcca
     2581 tgcttaattt tcctgggcca aaattaagca ccgcgttgcc cttacccgct tgcccctggg
     2641 tcagcacatt cagtcgtccg ctgaccagag cgttctcaag ccctgcctgc cagttgtcga
     2701 ctttgacacc cagtcggcca ctcaaaggaa aaccagcata cagccagctc cagcgaccat
     2761 cgcttacggt caattgttga cgagtaattt gccacggcaa atcgagcaac ggatcgccgt
     2821 tatcccgtgc cagcacgatc aattgcccgc tattttcctg ccagtccagc tcggcatcca
     2881 ccagtgacgg ttcctgcggc aagtttaacg tcgcagtagc atgcccactt acaggaagtc
     2941 catccggcac gagcggcata gtaaattccc ccaccagttt aaccggcggc tgattttcaa
     3001 acgcgacgac atccagttcg ctgactgtaa gttgttgccc tttcagctgg ccttgaaatt
     3061 taactttttc gccctgataa cgcagttgct ggatatcaga ggttaatgcg agagagagtt
     3121 ttccctgcca ttcctgccag ggagaaaaaa tcagtttatc gatattgatc caggtgttag
     3181 gcagcatgga ctgccactgc gcgagggttt ttggagcagc tggcgattgc tccgtctgcg
     3241 gcaatttcgc cagacaagca gaatcaagtt ctaccgtgcc gacgttcaat aaccagcggc
     3301 tgggatgtga aaggctggcg ttggtgatat gcgcaagctg acaatctccc accagataac
     3361 ggagatcggg aatgattaaa cctttacgcg taatgcgtgg gctttcatct aatgcaatac
     3421 gtgtcccgag cggtagccag atgtccgcca gcgtgggaac ccacagcccg agcgtcatca
     3481 gcagcgtcaa cggcacaaga ataatcagta ataacagcgc gagaacggct ttatatttac
     3541 ccagcatggg tagttaatat cctgatttag cgaaaaatta agcattcaat acgggtattg
     3601 tggcatgttt aaccgttcag ttgaaggttg cgcctacact aagcatagtt gttgatgaat
     3661 ttttcaatat cgccatggct ttcaattata tttgaaattt tgtaaaatat ttttagtagc
     3721 ttaaatgtga ttcaacatca ctggagaaag tcttatgaaa ctcgccgttt atagcacaaa
     3781 acagtacgac aagaagtacc tgcaacaggt gaacgagtcc tttggctttg agctggaatt
     3841 ttttgacttt ctgctgacgg aaaaaaccgc taaaactgcc aatggctgcg aagcggtatg
     3901 tattttcgta aacgatgacg gcagccaccc ggtgctggaa gagctgaaaa agcacggcgt
     3961 taaatatatc gccctgcgct gtgccggttt caataacgtc gaccttgatg cggcaaaaga
     4021 actggggctg aaagtagtcc gtgttccagc ctatgatcca gaggccgttg ctgaacacgc
     4081 catcggtatg atgatgacgc tgaaccgccg tattcaccgc gcgtatcagc gtacccgtga
     4141 cgctaacttc tctctggaag gtctgaccgg ctttactatg tatggcaaaa cggcaggcgt
     4201 tatcggtacc ggtaaaatcg gtgtggcgat gctgcgcatt ctgaaaggtt ttggtatgcg
     4261 tctgctggcg ttcgatccgt atccaagtgc agcggcgctg gaactcggtg tggagtatgt
     4321 cgatctgcca accctgttct ctgaatcaga cgttatctct ctgcactgcc cgctgacacc
     4381 ggaaaactac catctgttga acgaagccgc cttcgatcag atgaaaaatg gcgtgatgat
     4441 cgtcaatacc agtcgcggtg cattgattga ttctcaggcg gcaattgaag cgctgaaaaa
     4501 tcagaaaatt ggttcgttgg gtatggacgt ctatgagaac gaacgcgatc tgttctttga
     4561 agataaatcc aacgacgtga tccaggatga cgtattccgt cgcctgtctg cctgccacaa
     4621 cgtgctgttt accgggcacc aggcattcct gacagcagaa gctctgacca gtatttctca
     4681 gactacgctg caaaacttaa gcaatctgga aaaaggtgaa acctgcccga acgaactggt
     4741 ttaatcttgc agctcccctg caatccaggg gagctgattc agataatccc caatgacctt
     4801 tcattctcta ttcttaaaat agtcctgagt cagaaactgt aattgagaac cacaatgaag
     4861 aaagtagccg cgtttgttgc gctaagcctg ctgatggcgg gatgtgtaag taatgacaaa
     4921 attgctgtta cgccagaaca gctacagcat catcgctttg tgctggaaag cgtaaacggt
     4981 aagcccgtga ccagcgataa aaatccgcca gaaatcagct ttggtgaaaa aatgatgatt
     5041 tccggcagca tgtgtaaccg ctttagcggt gaaggcaaac tgtctaatgg tgaactgaca
     5101 gccaaagggc tggcaatgac ccgtatgatg tgcgctaacc cgcagcttaa tgaactcgat
     5161 aac
//
LOCUS       DAZPCS010000210         5150 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZPCS010000210 DAZPCS010000000
VERSION     DAZPCS010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5150
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.213"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            189..446
                     /locus_tag="ACHUQ7_003703"
                     /pseudo
     CDS             189..446
                     /locus_tag="ACHUQ7_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
     gene            complement(496..1446)
                     /gene="uspE"
                     /locus_tag="ACHUQ7_003704"
     CDS             complement(496..1446)
                     /gene="uspE"
                     /locus_tag="ACHUQ7_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFR1136915.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1598..2350)
                     /gene="fnr"
                     /locus_tag="ACHUQ7_003705"
     CDS             complement(1598..2350)
                     /gene="fnr"
                     /locus_tag="ACHUQ7_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFR1136916.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2545..3060)
                     /gene="ogt"
                     /locus_tag="ACHUQ7_003706"
     CDS             complement(2545..3060)
                     /gene="ogt"
                     /locus_tag="ACHUQ7_003706"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFR1136917.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3071..3214)
                     /locus_tag="ACHUQ7_003707"
                     /pseudo
     CDS             complement(3071..3214)
                     /locus_tag="ACHUQ7_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            3214..3430
                     /locus_tag="ACHUQ7_003708"
                     /pseudo
     CDS             3214..3430
                     /locus_tag="ACHUQ7_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885449.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(3604..4419)
                     /locus_tag="ACHUQ7_003709"
     CDS             complement(3604..4419)
                     /locus_tag="ACHUQ7_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR1136918.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGTVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHNYAITRQMI"
     gene            4516..>5150
                     /locus_tag="ACHUQ7_003710"
     CDS             4516..>5150
                     /locus_tag="ACHUQ7_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFR1136919.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWRVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLEG
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYTL"
BASE COUNT         1374 a         1175 c         1225 g         1376 t
ORIGIN      
        1 ctattttacc gcgacaattc attcagaaca tcaatagtca gggaaggaag tagcaacatt
       61 agctaactaa tcaatgcgtt gattgtaaat ccagctaaga ggtgaggttt tcagagcaga
      121 caacggtgaa acgtcatggt attgttacgt ttaggtaaca agaaatttgt ctgcacaagg
      181 attacatcat gattatggcg aaactgaagt cagcgaaagg gaagaaattt ctctttggtt
      241 tgttggcggt tttcattatt gcggcgtcgg ttgtgactcg cgcgaccatc ggcggcgtta
      301 tagaacagta caatattccg ctgtctgagt ggacgacatc aatgtatgtg atttagtcat
      361 cgatgatttt tgtttatagc ctggtcttta ctgtgttgct ggcaatcccg ttgggaattt
      421 atttccttgg cggcgaagag cagtaagtaa aaaataggcc cgataactcg ggccttgtca
      481 gttattgaag agtcgttaat cgtcttcttc gtcatccagt tcaacgggtg tctgatactg
      541 gtcaggttta atgaccagca ggtcgcagcg aagatgatca atcacctgtt ccgccgtgtt
      601 gccgaggaat gctgctgaaa taccggtgcg tccgaccgtg cccagaacca caattcccgc
      661 ctgtaaatgc tccgccaaat caggaatcac ctcttcgggc agaccttttt ctacgtgcgt
      721 catgttttca ttaatgccga atttctgccg cagggctttc attgccagca aatgttgccc
      781 acgaatggca tcgttataaa cgctcgggtc aaattccggc agttcaatcg cgatattaat
      841 tggcgttacc ggataagcgc caaccagatg aacttcggta tggttgactt gttctgccag
      901 ttcgatcgtc tctttgacca gtttttcatt gagcgcatta tgatacggct cttcactggc
      961 gagattcacc gccaccagcg ccttgcctcc ttccggccac ggctggtctt tcaccatcca
     1021 caccgggctt gggcatttgc gtaacagatg ccagtccgtt ggcgtaaaaa tcaccgcttc
     1081 cagacggtca tgttggtgcg ccatttttag caccaaatcg tgtccgccgc tgatcacttc
     1141 ctgaatgatg gcttcgaaag gacggttatg ccagaccact ttaatttcaa tgggaacgcc
     1201 agcattgaga taatattttg cctgctcgtg gatccaggct gtacgctggc tgatgacgcc
     1261 ctgacgcata gcggtacgtt cgtccgggga gagcagggtg gtcatttcgt atgagaagtc
     1321 atagatcggc aaaaaggctt taattttgcc accaatccgt tgatgtaaat aaacagctcg
     1381 ccgcaatgct ggttggtcgt cctggttagg atcgataaca acgagcatgt tctgatacat
     1441 agccatacag ggtctcctta caacaactgt caacgcagtt tgtaattaaa agattaaccc
     1501 atatctggtg aatgaaacag tgatgaacct tctgccagat caataaatca gaaaaattta
     1561 atgatatgac agaaggatag tgagttatgc ggaaaaatca ggcaacgtta cgcgtatggc
     1621 cagcaagctg ggccagcgca tcgttatttt cgatggtgat gtatttacct ttgactgcca
     1681 gcataccgct tttctggaag cgacccagca gacggctgat ggtttctacc gtcaggccca
     1741 gatagttacc aatatcgcca cgagtcatcg tcaggcggaa ttcacgaggg gagaagccgc
     1801 gttgggcaaa acgacgggac aggttgtaga tgaatgcagc cagacgttcc tcggcatttt
     1861 tcttcgacaa cagcaagatc atgtcctgat cgcctttgat ttcaccgctc atcagacgca
     1921 tcatctgctg acgcagattc ggcattttac cggacaaatc gtccagcgtt tcgaacggaa
     1981 tttcacatac catcgaggtt tccagcgcct gagcgaagct cgggtgatgg ccgctgccga
     2041 tggcgtcaaa tcccactagg tcgcctgcta aatggaaacc agtgatttgc tcgtcgcctt
     2101 gctcagtgat tgtataactt ttaatcgtac cggagcggat ggcataaagc gatttaagtt
     2161 catcaccagc cttaaacagc gtctggcctt tctgaatagg cttcttccgc tcaatgatat
     2221 tatcaagctg atcaagctca tgttcgttga gtgtgaacgg gatgcaaagc tggctgatgc
     2281 tgcaatcctg gcaatggata gcacaaccgc cagactgaat gcgccgtata attcgctttt
     2341 ccgggatcat aggtctgctc aagccgtaat tgatatttgt caattttaac atctttttag
     2401 ggagcaagta agtctaagca aaccttaaca gcagagaatt ccgatattag atgtaaatat
     2461 atgtctatct atttgaaact ccttaagttg tcaagggtaa ctttacataa aagtgtgaac
     2521 aagctggcac aaattgttta atgtttacag caaaagataa ccttcgtggc gcaataacca
     2581 ctcttttcgc tgaactccgc ctgcatatcc ggtcatggtg ccgtttcggc caataacccg
     2641 atggcaaggt acgacgatgc tgatgggatt cgatccgttt gccgcaccaa cggcacgcgc
     2701 cgcgccaggg cggcccaatt gttcagccag ttggccgtaa tgcattacct gcccgcaggg
     2761 gatagtgcgt agtgttttcc agacttcgcg ctgaaatggc gtcccccccg tggcagtggg
     2821 aagcgtatca ataatgctaa gattaccggc aaaatattca cgaagcttgt cgcttaaacc
     2881 acctgggttg gtggcagaaa tgcgctcata gccttctttg cgataatgga tgtccagcag
     2941 ctgcaccatg cgttcgctgt actcttccca ttcaaccgcc cgcaggcgaa attgctcatc
     3001 gcaaatcacc cacagtggac ccagtggcgt ggcaattttt tcttcaagta atctcagcat
     3061 ccgttctctc ttaagacaaa cgtgggtaaa taccctgacc tatcggcaga ccgacaagat
     3121 accacgccag caacatcagc agccatacca ccaaaaagat aagtggatag ggcaagacta
     3181 acgaatagta agtacccagt ttcgcgtctg gtttgtcgca gtctgatagc ataaagcgat
     3241 tttttaattt ttctgcctga agaaatgagg gcaagccatc acatggatcg agggtgggat
     3301 aattccagtc agtgaaattt taccgtaggc tatctgttat ttgattctgt ggcgtgatag
     3361 ccgccagaca ccactgaact cgtcactcat tccccaatcc cggcgagaat gtggttattt
     3421 acagagctaa aaaatgaata aaaaaataga gcctgtcgac atccgccaga ctctacagta
     3481 cacacagcag tgcatcctcg tcttaaatcc ggcctggggt cctggtgacc ggtgacagtg
     3541 ttataacggt tttacccatc cgttgccaat tttcttcagt gacagatttc acgaaaatat
     3601 aagttaaatc atttgtcttg tgattgcata attatgcatc gttaatcgac gagtaaattt
     3661 accgggcgag tagcccgttc gacgcttaaa catattgccg aacgattcag ctgaggcata
     3721 gcccagacga tgggcaattt tggtcaacga atatccatca tcaagaagcg tcactgcaga
     3781 aataacgtgc atttgctgaa cccagtggcg aaatgttaat cccgtctctt taacaaataa
     3841 gcgcatgagt gtacggctac tgacgttaat tttatccgct agattatcca gtgttggcag
     3901 atacccttgg ttgtttagca aattctcaca aagctgctgt agccgctcat catttggcca
     3961 agggagttca agtgacattt tcacccccat ttttatttcc tgaagtatca gtgtctcgag
     4021 ggctgaaaaa cgtaactgtt gtttatctgt ggtactttgc gacggtggaa gttggttaat
     4081 tgcaattagt aattccctta acaggggtga aatacccgtc atgaagcaaa ctgttcccag
     4141 cgtttcactt tccgcttctg aaaccagtac ggtttttaaa agtacgttgc ctgtcgcaac
     4201 aaacttatgc ggcatttgtg ggggcagcca aagcgcgctg ctggcatgga ctagccagac
     4261 gctttctcca gcccatacac gaacgactcc ttcagccggg taaagcaact gggcatagag
     4321 atgtttatgc cagggaactg tacctccatt cagatgttcc gaatccagtc cgataatgtg
     4381 atgccctggt gatgctttgg gttgcagtgt aattgtcatt tacagtaggt attttgtcag
     4441 aaatcatcaa ataagccatg ttaaatttta aatatgacgt atgcaagttt ccggatatat
     4501 acggtgtaaa gagtgatgat aatttctaaa aaacagctta ttggggtggt agcgatcggt
     4561 attcttttga ctggcgttgt cttttttatc tggagggtaa gcaaaggaag atttatacag
     4621 acgacggatg atgcttacat tggtggtaat atcaccacgg tggcaagtaa agtttctggt
     4681 tatatttctg ctatagaggt gcgcgataat cagagcgtaa aaaaaggcga tattatcctg
     4741 cgtctcgatg atcgggatta tcgtgcaaat gtcgccaggc ttgaggcaaa aataaaaagt
     4801 agcaaagcca atctcgaagg cattcaagcc actattacga tgcagcagtc gataattcaa
     4861 agtgccagtg aaacatggca ggcagtaaaa catgaggagc aaaagcgcct gcgagatact
     4921 gaacgttatg aaaaactcgc acaatcagcg gcaatatcgc aacaaattat tgataacgcc
     4981 aggtttgact accagcaggt cgcagcaaag gagcgcaaag ccgcgaacga tttcctggtg
     5041 gagaagcaac ggctggcagt tttatctgct caggaagaaa acgtacgtgc gagtatcgaa
     5101 gaagttcagg ccgctttgac tcaggcacta ctggatctgg aatataccct
//
LOCUS       DAZPCS010000211         4992 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZPCS010000211 DAZPCS010000000
VERSION     DAZPCS010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4992
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.214"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..376)
                     /gene="ipgA"
                     /locus_tag="ACHUQ7_003711"
     CDS             complement(<1..376)
                     /gene="ipgA"
                     /locus_tag="ACHUQ7_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HFR1136920.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANIT"
     gene            complement(389..1873)
                     /gene="icsB"
                     /locus_tag="ACHUQ7_003712"
     CDS             complement(389..1873)
                     /gene="icsB"
                     /locus_tag="ACHUQ7_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HFR1136921.1"
                     /translation="MILKISNFIDASNTKGPIRVEDTEHGPILIAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLENQKSLLLFLNTLASEKSAESAFAAYEAAKNSIQHSF
                     TGRDIKLMLNTAERFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGNVTKDTEINYLFEKRSGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISHNLFGLDE
                     TNMSKFICKKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NDVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDAVINACHSYLVKSAPSNMTRVLNELKTEATDKKEE
                     IIEKSIKIIDYYNSLKSPDLGTKLYIHDLLQINKLLLNNSHSNI"
     gene            2187..3803
                     /locus_tag="ACHUQ7_003713"
     CDS             2187..3803
                     /locus_tag="ACHUQ7_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HFR1136922.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGKHKGVSVISYQRVKNGER
                     NKGIEALNRLYLQNQTSLTGKSLLFARDRAEVFCEAIKLAGGDTSKIKAMMERLDTYK
                     LGEVNKRHINELNKVISEEIRAQLGIKNKKELQTKIKQIFTDYLNNKNWGPVDKNISH
                     HGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESDHIANMWLSKVVDDEGKE
                     IFSGIRHGVISAYGLKKNSSERAVAARNKAEELVSAALYSRPELLSQALSGKTVDLKI
                     VSTSLLTPTSLTGGEESMLKDQVNALKGLNSKRGKPTKLLIRNSDGLLKEVSVNLKVV
                     TFNFGVNELALKMGLGWRNVDKLNDESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKN
                     DVIYLANQIKEIVTKKLQKNDNGEPYKLSQRMTLLAYTIGAVPCWNCKSGKDRTGMQD
                     AEIKREIIRKHETGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKK
                     LPLSSLELSYSERIGDPKIWNMVKGYSSFV"
     gene            3813..4175
                     /gene="ipgE"
                     /locus_tag="ACHUQ7_003714"
     CDS             3813..4175
                     /gene="ipgE"
                     /locus_tag="ACHUQ7_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HFR1136923.1"
                     /translation="MEDLADVICRALGIPLIDIDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGNLIARLDLTGINEFEDVYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            4175..4633
                     /gene="ipgF"
                     /locus_tag="ACHUQ7_003715"
     CDS             4175..4633
                     /gene="ipgF"
                     /locus_tag="ACHUQ7_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system lytic transglycosylase
                     IpgF"
                     /protein_id="HFR1136924.1"
                     /translation="MSRFVFILLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKSAVNVNNNGSKDYGIMQINDFHSKRLREMGYSEEMLISHPCLSVHYAAKLLNEFM
                     MMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYRRYLRIAAESKQNNRRI"
     gene            4642..>4992
                     /gene="mxiG"
                     /locus_tag="ACHUQ7_003716"
     CDS             4642..>4992
                     /gene="mxiG"
                     /locus_tag="ACHUQ7_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HFR1136925.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLTISKDNICQFSDEKGEQIDINSQFNSFEYDGIS
                     FHLKNMREDKSRGHI"
BASE COUNT         1659 a          673 c         1043 g         1617 t
ORIGIN      
        1 aagtgatgtt tgcaatggtt agtttaatac aaaataaaaa ttcatacaaa gattcaatga
       61 gttcatcagg tgaaagatat tttttgtagt gtttcttggt caaaacacat tgatgagtat
      121 cagggagaag acagatagaa gaaccataat ctctactcaa agagtagtta aggatgagta
      181 tgtgttctgg attaataata ttttctgtaa aaagttcatg ttcattaaga agtccggtca
      241 aaaagatgta ttcatcatta tctgttatac aaacaggtat ttgttcttcg agcaatataa
      301 aggcttgatt tttatcgtta aaatcaagct ttgcacctgt tatttcataa agtttatcat
      361 atagtttgcg acacatttct atggccttct atatattaga atgagagtta ttcaataaaa
      421 gcttattgat ctgtaataag tcgtgaatgt aaagtttggt tccaagatct ggcgatttaa
      481 gagaattgta ataatcaata atttttattg atttttcgat gatttcttct tttttatctg
      541 ttgcttcggt ttttaattcg tttaatacac gagtcatatt gctgggtgct gattttacta
      601 agtatgagtg gcaagcgttg atgaccgcat caaagtcttc tgaagataca ttttcagtag
      661 gatgtttgta aagtgaaatt gataaaggct tgaatgtttt tggtgtgtgg ataaggttaa
      721 ttttattgaa acttttaaga tagctgcgtc gtaattgagt caatctggaa agatcaaaat
      781 cagattcaat atacttcatt atttcgttat aagactgatt gagactttca atgcgttgcc
      841 taactttatt tgcatacgcg tatacatcgt taggtgtagc aaccaattta acgtcaggga
      901 gtggaaagta tatttcagaa tttcctgctt taagaacatt aagtgccata ccagcacagt
      961 tttcagattt actgattaat ttataatttg ccaattgtct gaaagcatct gctttctttt
     1021 tgcatataaa ttttgacatg tttgtttcat caaggccaaa aaggttatgg ctgattttac
     1081 catcctttgt tttattatca cctgataatg gaatgtatac tttatcagca ctgacccccc
     1141 aataaccata ggggtctttt ttttgattag ctctgggaaa aaatattttt ttgttgttat
     1201 tttgatcttg ttttgaattt aataagtttc taacctcttg gcctgcatca agtctttcgg
     1261 ctgtttggtc tgaaatcata tttagtttgt cttgcttata ggtatcgaca ctgtaacctg
     1321 agcgtttttc aaaaaggtag tttatttctg tatcttttgt aacattcccg ccgggatacc
     1381 agctgagata ggtatgagtt ggttcctgta gtaaattgtt ttttatactg atactagtat
     1441 gccctaaatg ggttatgcct ctatttcccc agcaacgaaa aactaaatgt ctttcaagat
     1501 ttttggctgt accgatgcca tgaaaacgct ctgcggtatt caacattaat ttgatgtctc
     1561 tacctgtgaa agagtgttga attgagtttt tagctgcttc ataggcggca aacgcacttt
     1621 ctgcagattt ttcgctagct aatgtattga ggaaaagcaa taaggatttt tgattctcta
     1681 gtcgataatt ttttagttgt tcatttaata tctgtgaatt tatctttgca ttaatagtgc
     1741 ttaatgttct gaaaaagaga tcctttaggt tgaatttctg tgctatcaat ataggtccat
     1801 gctctgtatc ttcaacgcgt ataggccctt ttgtattgct tgcgtcaatg aaattgctaa
     1861 ttttgaggat catactttat taactctcca ttacttggtg attgtgggga tgtaaaatgt
     1921 atttggtata taatatattc aattaataaa tttagaaact tgagcctgtt aacataatca
     1981 aattttcttt tgctgtacat aatatgtacc tcgtgagcat atgtagtgct cgtttcatca
     2041 tgaaatccca caagataaag tgcctgatgt atcaggctcg gagtgttata gaaaaagaga
     2101 gaaccctgtt gaataagtat cttttttgtt gtaaaacgag gtacaaaact gcaatcaatg
     2161 ccccatttat gactgaggat aattaaatgc acataactaa tttgggattg catcaggttt
     2221 catttcaaag cggagattcc tataaaggcg cagaagaaac cggaaagcac aaaggtgtaa
     2281 gcgtgatttc atatcaaaga gttaaaaatg gagaaagaaa taaggggatc gaagcgctta
     2341 atcgattata tttacagaac caaacaagct taacaggaaa aagccttttg tttgcgcgtg
     2401 acagagctga agttttttgc gaggcaataa agttggcagg tggtgatacg tcaaaaatta
     2461 aagccatgat ggaacgatta gatacctata aacttggtga agttaataaa agacatatta
     2521 atgagcttaa taaagtaata agtgaagaga taagagcaca gctaggcatt aaaaataaaa
     2581 aggaattgca aacgaaaata aaacaaatat ttactgatta tttaaataat aagaattggg
     2641 gaccggtcga caagaatatt agtcatcatg ggaaaaatta tggttttcaa ttaactcctg
     2701 cctctcatat gaagataggg aataaaaata tatttgtcaa agagtataat ggaaaaggaa
     2761 tttgttgtgc ttctacgaga gagagtgatc atatcgcgaa tatgtggctt tcaaaagtgg
     2821 tggatgatga ggggaaggaa attttttcgg gtatcagaca tggagttata tcagcctatg
     2881 gattgaaaaa aaactcatct gaaagagctg ttgctgctcg taataaagct gaggagttag
     2941 taagcgcagc attatatagc agaccagagt tattatcaca ggctttgtct ggtaaaacag
     3001 tagatttaaa gattgtttcg acttctcttc tgacgccgac cagtttaacc gggggagagg
     3061 agagtatgtt aaaagatcag gtgaacgcat taaaagggtt aaactctaaa agggggaagc
     3121 caacgaaatt attaattcgg aatagtgatg gtcttctgaa agaagtaagc gttaatctaa
     3181 aagtggtgac atttaacttt ggtgtaaatg aattagcgct aaaaatgggc ttaggctgga
     3241 ggaatgttga caagcttaat gatgaatcaa tatgttcttt gttgggggat aatttcctca
     3301 aaaatggtgt gattggaggt tgggctgcag aagcaataga aaaaaatcca ccatgtaaga
     3361 atgatgtgat atatctggct aaccagataa aagagattgt aaccaaaaag ttacaaaaaa
     3421 atgataatgg agagccgtat aaattgtcac aaagaatgac tctattggct tatactatag
     3481 gtgctgtacc ttgctggaat tgtaagagtg ggaaggacag aacaggtatg caggatgctg
     3541 aaattaaaag agaaataata agaaaacatg aaacaggtca gttttctcaa ttaaatagta
     3601 aattatcctc agaagagaaa agattatttt ctactattct aatgaatagt ggtaatatgg
     3661 aaatccaaga gatgaatact ggtgtgcccg gaaataaagt catgaaaaaa ttgccgctat
     3721 cttcgttaga gctatcttat tctgaaagaa taggggaccc aaaaatatgg aatatggtga
     3781 aagggtattc gtcatttgta taagaggaat atatggaaga tttagcagat gttatttgcc
     3841 gagccttggg tatcccttta attgatatcg atgatcaagc aatcatgctt gatgatgatg
     3901 tgctaatata tattgaaaaa gaaggagatt caataaattt attatgccct ttttgtgcat
     3961 tgcctgaaaa tataaatgat cttatatatg cgttgagcct aaattactca gaaaagatat
     4021 gcttagctac tgatgatgaa ggcggaaatc taattgcgcg attagatctt accgggataa
     4081 atgaattcga ggatgtatat gttaatacag aatattatat ttcgcgtgtg cgttggctga
     4141 aggatgaatt tgcgcgaaga atgaaggggt attaatgtcc cgtttcgtat ttattttgct
     4201 gtgttttatc ccatatttag ggagagctga ttgttgggat aaggctggtg aaaggtacaa
     4261 tattccatca agtttgttaa aagcgattgc ggaaaaagaa tccggattta ataaatctgc
     4321 agtcaatgtt aataacaatg gaagtaaaga ttatggtata atgcaaataa atgactttca
     4381 ttctaaaaga cttagagaaa tgggatattc tgaggaaatg ctaattagtc atccatgtct
     4441 ttctgtacat tatgcagcaa agttattaaa tgagtttatg atgatgtatg gaagagggtg
     4501 ggaggctgtt ggagcctata atgcaggaac gtctccgaaa aagaaaaaag aaagattaaa
     4561 gtacgcagag gatatctata gacgctattt gcggatagca gcagagagca agcagaataa
     4621 tcgaaggata taagaggatt tatgtctgag gcaaagaact caaatcttgc accattcagg
     4681 ttattagtga aactcacaaa cggtgtcggg gatgagtttc cgttatatta tgggaataac
     4741 ttaattgttc tcggacggac gatagaaaca cttgagtttg gaaacgacaa tttcccagaa
     4801 aatattattc ctgtaacaga tagtaaatcg gatggtataa tttatttgac cataagcaaa
     4861 gataatatct gtcaatttag tgatgaaaaa ggtgagcaaa ttgatatcaa ttcacaattt
     4921 aattcatttg aatatgacgg gatttctttt caccttaaaa atatgaggga ggataaatcg
     4981 agaggccata ta
//
LOCUS       DAZPCS010000212         4973 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZPCS010000212 DAZPCS010000000
VERSION     DAZPCS010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4973
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.215"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(186..770)
                     /gene="kptA"
                     /locus_tag="ACHUQ7_003717"
     CDS             complement(186..770)
                     /gene="kptA"
                     /locus_tag="ACHUQ7_003717"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HFR1136926.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADINKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVVLTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEWSFMPTGVQKQ"
     gene            1020..1703
                     /locus_tag="ACHUQ7_003718"
     CDS             1020..1703
                     /locus_tag="ACHUQ7_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFR1136927.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFPGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            1700..2161
                     /locus_tag="ACHUQ7_003719"
     CDS             1700..2161
                     /locus_tag="ACHUQ7_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFR1136928.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            2174..3346
                     /gene="iadA"
                     /locus_tag="ACHUQ7_003720"
     CDS             2174..3346
                     /gene="iadA"
                     /locus_tag="ACHUQ7_003720"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFR1136929.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            3411..4322
                     /gene="hypT"
                     /locus_tag="ACHUQ7_003721"
                     /gene_synonym="qseD"
     CDS             3411..4322
                     /gene="hypT"
                     /locus_tag="ACHUQ7_003721"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HFR1136930.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMVRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="ACHUQ7_003722"
     CDS             complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="ACHUQ7_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HFR1136931.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(4704..4787)
                     /gene="idlP"
                     /locus_tag="ACHUQ7_003723"
     CDS             complement(4704..4787)
                     /gene="idlP"
                     /locus_tag="ACHUQ7_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HFR1136932.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            complement(4903..>4973)
                     /locus_tag="ACHUQ7_003724"
     CDS             complement(4903..>4973)
                     /locus_tag="ACHUQ7_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1136933.1"
                     /translation="NYSEYSEYTSVFFLSLMIFIKS"
BASE COUNT         1177 a         1305 c         1238 g         1253 t
ORIGIN      
        1 gtatgaccgg aagcgacgct gattccagtc cccaactgac cgcctgccgt aaatcacttt
       61 aatttcagca gttgttatta aaggataccc ttttacgagg gtatcccttc agactgataa
      121 caaacgctac attgccggat gtgctgcgct taccaggcct atataacccc tgcaatatat
      181 tgaatttatt gtttttgtac cccagtcggc ataaacgacc actcaagaaa ctcaacagca
      241 accgttgatg tcagccagac cccgttttcc gcttgccaga aagggatgcc tcgtttcgcc
      301 atttcctgtg ctttgacggt taagacaacc ggcgatccgt gtcttgctcc cactttgcgt
      361 gccgttgctt catcagcaga gagatgcacg taatgacgat cccctgcaat cagcccctgt
      421 ttttttatct cgtcaaggaa acggcttgcc gtaccgtggt agagaaactg tggcggtgtt
      481 ttttcggcaa aggaaatagc gacctgcgaa gtcgaatgcc cctgaaccgc gcggatgcac
      541 ctaccatcac tggaataact aaaacgcttt ttatcgctgg ttgcgaccac ggtatccagc
      601 aatgcacggg ttaatcgctt gcccgctttt tgggcacaaa gtatgagctt attgatatcc
      661 gcccatcctt cacggtccag tacgatacca atcgcctccg gtttgtgtcg gaggacaaaa
      721 cttaaaaact tgctcgtatc cgccagctct ttttcgttgt actttgccat cgcgttttcg
      781 tccctggatt ttctgttttt atttactaat gtcattgtca ctgggtttat gaattttctg
      841 caactcaatc ctgcgcagaa ccttttccac acagcgtaat ttttccccta tgcaaaaaac
      901 gcatagccag aaacatactt gcattttccc catacccaca tggctgataa ggtttttcag
      961 ttccaggaat tttcctgact caagcaacac acaaacacaa aaaaacaatg gggattgtta
     1021 tgacacaaca aggggatgct gtagccgggg agctggcgac agaaaaagtg ggcattaaag
     1081 ggtatctggc tttttttctc accataatct tttttcccgg tgttttttcc ggaacagaca
     1141 gctggtggcg cgtttttgat ttttccgttc tgaacggatc gtttgggcaa ctgcctggtg
     1201 cgaatggagc caccacctca tttcgcgggg tgggtggcgc gggggctaaa gatggctttc
     1261 tctttgcgct ggagctggca ccgtcagtca ttctctctct ggggattatt tcaattaccg
     1321 atggtcttgg cggattacgc gccgcgcagc agttaatgac acctgtgcta aaaccgttgt
     1381 tgggcattcc agggatctgc tcgctggcac tgatcgctaa cctgcaaaac acggatgccg
     1441 ccgccggtat gaccaaagag ctggcgcagg aaggtgaaat taccgaacgc gataaagtga
     1501 ttttcgccgc atatcaaacc agcggtagcg ccatcatcac caactacttc tcttccggcg
     1561 tcgcggtgtt tgcctttctg ggcacgtccg taatcgtacc tttagccgtc atcctggtgt
     1621 ttaaatttgt cggagccaac attttgcgcg tctggcttaa cttcgaagaa cgtcgcaacc
     1681 caacgcaagg agcgcaagca tgacaactca ggtccgcaaa aacgtcatgg atatgtttat
     1741 cgatggcgcg cgccgtggct ttactatcgc cacgaccaac ctgctgccaa acgtggtgat
     1801 ggcgtttgtc atcattcagg cgctgaaaat taccggcctg ctcgactggg tggggcatat
     1861 ctgtgaaccc gtaatggcgc tgtggggatt acccggtgaa gcggcaaccg tccttttagc
     1921 cgcattaatg agtatgggcg gcgcagtcgg tgttgccgcc agtctggcaa ccgctgacgc
     1981 attaaccggc cacgatgtca ccgttttact gcccgcaatg tatttaatgg gcaacccggt
     2041 gcaaaatgtc ggtcgctgcc tggggacagc cgaagtgaat gccaaatatt acccgcatat
     2101 cattacggtc tgcgtgatta acgcattgct gtcgatctgg gttatgcagc ttattgttta
     2161 acaaggagtt accatgattg attataccgc agccggtttt accctgctgc agggagcgca
     2221 tttgtatgcg ccggaagatc ggggaatttg cgatgtcctc gtcgctaacg gcaaaattat
     2281 cgccgttgcc agcaatatcc cttctgacat tgtaccgaac tgcacggttg tcgatctcag
     2341 cgggcagatc ctctgcccag gttttattga tcaacacgtc catttgattg gcggtggcgg
     2401 cgaagcaggt cccacgacgc gcacgccgga agtggcgcta agtcgcctga cggaagcggg
     2461 cgtcacgtca gtggttggtc tgctgggcac cgactctatc tctcgccacc cggaatccct
     2521 gctcgccaag acccgtgcgc tcaatgaaga aggcatcagc gcctggatgc tgaccggcgc
     2581 ttatcatgtc ccttcccgca ctattacggg ttccgtggaa aaagacgtgg cgattatcga
     2641 tcgtgtgatt ggcgtgaaat gcgccatctc tgatcaccgt tctgccgcac cggacgttta
     2701 tcacctggct aatatggcgg cagaatcccg cgttggcggt ttgctcggcg gtaaacctgg
     2761 cgtcaccgtg ttccacatgg gcgacagtaa aaaggcgtta aagcctattt atgacctgct
     2821 ggaaaactgc gatgtgccga tcagcaagct gctgccgacc cacgttaacc gcaacgtacc
     2881 gttgtttgag caggcgctgg agttcgcgcg caaaggcggc accatcgata tcaccagcag
     2941 cattgacgaa ccggtcgccc ctgccgaagg tattgcccgc gccgttcagg cgggtattcc
     3001 gctggcacgc gtcaccctca gctccgacgg caacggtagc cagccgttct tcgatgacga
     3061 agggaattta acccatatcg gtgttgcagg ttttgaaacg ttgctggaaa ccgtgcaggt
     3121 gctggtcaaa gactatgatt tcagtatcag cgatgccctg cgcccgctca ccagtagcgt
     3181 agccggtttc cttaacatga gcgggaaagg cgaaattctg ccaggcaatg acgcagactt
     3241 actggtcatg acgccagaat tgcgcattga gcaggtatac gctcgcggca aactgatggt
     3301 caaagacggc aaagcctgcg tgaaaggaac gtttgaaacg gcttaaacgg ttgtcagccg
     3361 ccctaatgga cgtagaatgc ccccaagggc ggctgacaga gtaaaacgta atggatgact
     3421 gtggtgcggt tttgcataat attgaaacca aatggcttta tgattttctg actctggaaa
     3481 aatgccgcaa tttttcccag gcggcagtca gtcgcaacgt ctcgcaaccg gcattcagcc
     3541 gccgcatccg tgcactggaa caggcgattg gcgttgaatt gtttaaccgc caggtgacgc
     3601 cactgcaact ctcagaacaa gggaaaatct tccattcgca gatccgccat ctgttgcaac
     3661 agttagaaag caaccttgca gagctgcgtg gcggcagcga ttacgcgcaa cgtaaaatca
     3721 agatagccgc tgcacactct ctttccctcg gtctgttacc gtccattatc agccagatgc
     3781 cgccgctctt tacctgggca attgaagcca ttgatgtcga tgaagcggtc gataaactgc
     3841 gtgaagggca aagtgactgt attttttcct ttcacgacga agatttgctg gaagcgccgt
     3901 ttgaccacat tcgcttattt gaatctcaat tgttccctgt ctgcgccagt gacgaacacg
     3961 gagaagcact ttttaacctc gcgcagccac actttccgtt actgaattac agccgcaact
     4021 cctacatggt gcgattgatt aatcgcaccc tgacgcgcca cagtgagtta agtttcagca
     4081 ccttttttgt ctcttcgatg agcgagcttt taaagcaggt tgccctcgac ggctgtggga
     4141 ttgcctggct gccggagtat gccatccaac aagaaattcg cagcgggcag ctcgttgtgc
     4201 tgaatcggga cgaacaggtg atcccgattc aggcctacgc ataccggatg aatacccgca
     4261 tgaatccagt tgccgaacgc ttctggcgtg aactgcgcga gctggagatt gtgcttagct
     4321 gacattctca agcgtcgcac tgcgccaacc gccattatcc cagaccagat caaacgttag
     4381 cggttcatcg tcgtggtaca gcattatctc aaggctgatc gccagagtgt ggcagtacgc
     4441 ttctttcaaa ttaacctgca acgccgcaat ccgtggctca tgaaataaca gtgcagaacg
     4501 cagctgacgg caataccagc tctggagatg tttgtcgctc gggttatgca cttgccactc
     4561 gggcatgcct gaaacaagta atgacggtaa tgccgaatga ggagaattaa aaagagtgag
     4621 gagatcgctg cataccgatt tacgcagcaa tgacgtttta ttgcctgaaa tttcaggtaa
     4681 aacagcctga agtgattggc gcatcatttt gcgcactcct gacgtttagc aacatatgcg
     4741 gcttgccctg aacaccgggc accactgtat tgatgctcat tttccatagc cattcccttt
     4801 tgttcattac atccatgtca gtctgagaca ggcctgaatc ctgtcagcaa agtataaaac
     4861 cttacttgcc tatgaatatc tactgacttt tcaggaaaaa agtcatgatt tgataaaaat
     4921 catcaaactc aaaaaaaaaa cgctagtata ttctgaatat tccgaatagt tac
//
LOCUS       DAZPCS010000213         4966 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZPCS010000213 DAZPCS010000000
VERSION     DAZPCS010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4966
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.216"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            238..1182
                     /gene="leuO"
                     /locus_tag="ACHUQ7_003725"
     CDS             238..1182
                     /gene="leuO"
                     /locus_tag="ACHUQ7_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFR1136934.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            complement(1337..1381)
                     /locus_tag="ACHUQ7_003726"
     CDS             complement(1337..1381)
                     /locus_tag="ACHUQ7_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1136935.1"
                     /translation="MKRNDKILHYEGLN"
     gene            1500..3224
                     /gene="ilvI"
                     /locus_tag="ACHUQ7_003727"
     CDS             1500..3224
                     /gene="ilvI"
                     /locus_tag="ACHUQ7_003727"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFR1136936.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPRATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGMGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3227..3718
                     /gene="ilvN"
                     /locus_tag="ACHUQ7_003728"
     CDS             3227..3718
                     /gene="ilvN"
                     /locus_tag="ACHUQ7_003728"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFR1136937.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            3730..3816
                     /locus_tag="ACHUQ7_003729"
     CDS             3730..3816
                     /locus_tag="ACHUQ7_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFR1136938.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            3898..4902
                     /gene="cra"
                     /locus_tag="ACHUQ7_003730"
     CDS             3898..4902
                     /gene="cra"
                     /locus_tag="ACHUQ7_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFR1136939.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
BASE COUNT         1207 a         1133 c         1308 g         1318 t
ORIGIN      
        1 cggtagttat gattagattg ttttcgcaac aaaacattat ggattattat gctgtggtaa
       61 atgactcatt ccacggcaat ggattctgtt tttatcagaa cccgtatctt tatgttttcc
      121 gaattttact cattttgctt tttcttattt tatatgcatg ataaatcata ttcttcagga
      181 ttatttctct gcattccaat aagggaaagg gagttaagtg tgacagtgga gttaagtatg
      241 ccagaggtac aaacagatca ttcagagacg gcggagttaa gcaagccaca gctacgcatg
      301 gtcgatctca acttattaac cgttttcgat gccgtgatgc aggagcaaaa cattacccgt
      361 gccgctcatg ttctgggtat gtcgcaacct gcggtcagta acgctgttgc acgcctgaag
      421 gtgatgttta atgacgagct ttttgttcgt tatggccgtg gtattcaacc gactgctcgc
      481 gcatttcaac tttttggttc agttcgccag gcattgcaac tagtacaaaa tgaattgcct
      541 ggttcaggtt ttgaacccgc gagcagtgaa cgtgtatttc atctttgtgt ttgcagcccg
      601 ttagacagca ttctgacctc gcagatttat aatcacattg agcagattgc gccaaatata
      661 catgttatgt tcaagtcttc attaaatcag aacactgaac atcagctgcg ttatcaggaa
      721 acggagtttg tgattagtta tgaagacttc catcgtcctg aatttaccag cgtgccatta
      781 tttaaagatg aaatggtgct ggtagccagc aaaaatcatc caacaattaa aggcccgtta
      841 ctgaaacatg atgtttataa cgaacaacat gcggcggttt cgctcgatcg tttcgcgtca
      901 tttagtcaac cttggtatga cacggtagat aagcaagcca gtatcgcgta tcagggcatg
      961 gcaatgatga gcgtacttag cgtggtgtcg caaacgcatt tggtcgctat tgcgccgcgt
     1021 tggctggctg aagagttcgc tgaatcctta gaattacagg tattaccgct gccgttaaaa
     1081 caaaacagca gaacctgtta tctctcttgg catgaagctg ccgggcgcga taaaggccat
     1141 cagtggatgg aagaacaatt agtctcaatt tgcaaacgct aactgaatgc agaataggcc
     1201 agacatgaat gtctggttta ttctgcattt tttattgaat gtagaatttt attctgaatg
     1261 tgtggactct ctattttagg attaattaaa aaaatagaga aattgctgta agttgtggga
     1321 ttcagccgat ttattatcaa tttaatcctt cgtaatggag gattttatcg tttcttttca
     1381 cctttcctcc tgtttattct tattaccctg cgtttatgtc tctggctgcc aattgcttaa
     1441 gcaagatcgg acggttaatg tgttttacac attttttccg tcaaacagtg aggcaggcca
     1501 tggagatgtt gtctggagcc gagatggtcg tccgatcgct tatcgatcag ggcgttaaac
     1561 aagtattcgg ttatcccgga ggcgcagtcc ttgatattta cgatgcattg cataccgtgg
     1621 gtggtattga tcatgtatta gttcgtcatg agcaggcggc ggtgcatatg gccgatggcc
     1681 tggcgcgtgc gaccggggaa gtcggcgtcg tgctggtaac gtcgggtcca agggcgacca
     1741 atgcgattac cggcatcgcc accgcttata tggattccat tccattagtt gtcctttccg
     1801 ggcaggtagc gacctcgttg ataggttacg atgcctttca ggagtgcgac atggtgggga
     1861 tttcgcgacc ggtggttaaa cacagttttc tggttaagca aacggaagac attccgcagg
     1921 tgctgaaaaa ggctttctgg ctggcggcaa gtggtcgccc aggaccagtg gtcgttgatt
     1981 taccgaaaga tattcttaat ccggcgaaca aattacccta tgtctgccct gagtcggtca
     2041 gtatgcgttc ttacaatccc actactaccg gacataaagg gcaaattaag cgtgctctgc
     2101 aaacgctggt agcggcaaaa aaaccggttg tctacgtagg cggtggggca atcatggcgg
     2161 gctgccatca gcagttgaaa gaaacggtgg aggcgttgaa tctgcccgtt gtttcctcat
     2221 tgatgggaat gggggcgttt ccggcaacgc atcgtcaggc actgggcatg ctgggaatgc
     2281 acggtaccta cgaagccaat atgacgatgc ataacgcgga tgtgattttc gccgtcgggg
     2341 tacgatttga tgaccgaacg acgaacaatc tggcaaagta ctgcccaaat gccactgttc
     2401 tgcatatcga tattgatcct acttccattt ctaaaaccgt gactgcggat atcccgattg
     2461 tgggggatgc tcgccaggtc ctcgaacaaa tgcttgaact cctgtcgcaa gaatccgccc
     2521 atcaaccgct ggatgagatc cgcgactggt ggcagcagat tgaacagtgg cgcgctcttc
     2581 agtgcctgaa atatgacact cacagtgaaa agattaaacc gcaggcggtg atcgagactc
     2641 tttggcggtt gactaatggg gacgcttacg tgacgtccga tgtcgggcag caccagatgt
     2701 ttgctgcgct ttattatcca ttcgacaaac cgcgtcgctg gatcaattcc ggcggtctcg
     2761 gcacgatggg ttttggttta cctgcggcac tgggcgtcaa aatggcgttg ccagaagaaa
     2821 ccgtggtttg cgtcactggc gacggcagta ttcagatgaa catccaggaa ctgtctaccg
     2881 cgttgcaata cgagttgccc gtactggtgg tgaatctcaa taaccgctat ctggggatgg
     2941 tgaaacagtg gcaggacatg atctattccg gccgtcattc acaatcttat atgcaatcac
     3001 tacccgattt cgtccgtctg gcggaagcct atggtcacgt cgggatccag atctctcatc
     3061 cgcatgagct ggaaagcaaa cttagcgagg cgctggaaca ggtgcgcaat aatcgcctgg
     3121 tgtttgttga tgttaccgtc gatggcagtg agcacgtcta cccgatgcag attcgcgggg
     3181 gcggaatgga tgaaatgtgg ttaagcaaaa cggagagaac ctgattatgc gccggatatt
     3241 atcagtctta ctcgaaaatg aatcaggcgc gttatcccgc gtgattggcc ttttttccca
     3301 gcgaggctac aacattgaaa gcctgaccgt tgcgccaacc gacgatccga cattatcgcg
     3361 tatgaccatc cagaccgtgg gcgatgaaaa agtacttgag cagatcgaaa agcaattaca
     3421 caagctggtc gatgtcttgc gcgtgagtga gttggggcag ggcgcgcatg ttgagcggga
     3481 aatcatgctg gtgaaaattc aggccagcgg ttacgggcgt gacgaagtga aacgtaatac
     3541 ggaaatattc cgtgggcaaa ttatcgatgt caccccctcg ctttataccg ttcaattagc
     3601 aggcaccagc gataagcttg atgcattttt agcatcgatt cgcgatgtgg cgaaaattgt
     3661 ggaagttgct cgctctggtg tggtcggact ttcgcgcggc gataaaataa tgcgttgaga
     3721 atgatctcag tgcgcaattt gcagcccaac atgtcacgtt gggctttttt tgcgaaatca
     3781 gtgggaacct ggaataaaag cagttgccgc agttaatttt ctgcgcttag atgttaatga
     3841 atttaaccca taccagtaca atggctatgg tttttacatt ttacgcaagg ggcaattgtg
     3901 aaactggatg aaatcgctcg gctggcggga gtgtcgcgga ccactgcaag ctatgttatt
     3961 aacggcaaag cgaagcaata ccgtgtgagc gacaaaaccg ttgaaaaagt catggctgtg
     4021 gtgcgtgagc acaattacca cccgaacgcc gtggcagctg ggcttcgtgc tggacgcaca
     4081 cgttctattg gtcttgtgat ccccgatctg gagaacacca gctatacccg catcgctaac
     4141 tatcttgaac gccaggcgcg gcaacggggt tatcaactgc tgattgcctg ctcagaagat
     4201 cagccagaca acgaaatgcg ctgcattgag caccttttac agcgtcaggt tgatgccatt
     4261 attgtttcga cgtcgttgcc acctgagcat cctttttatc aacgctgggc taacgacccg
     4321 ttcccgattg tcgcgctgga ccgcgccctc gatcgtgaac atttcaccag cgtggttggt
     4381 gccgatcagg atgatgccga aatgctggcg gaagagttac gtaagtttcc cgccgagacg
     4441 gtgctttatc ttggcgcgct gccggagctt tctgtcagct tcctgcgtga acaaggtttc
     4501 cgtactgctt ggaaagatga tccgcgcgaa gtgcatttcc tgtatgccaa cagctatgag
     4561 cgggaggcgg ctgcccagtt attcgaaaaa tggctggaaa cgcatccgat gccgcaggcg
     4621 ctgttcacaa cgtcgtttgc gttgttgcaa ggagtgatgg atgtcacgct gcgtcgcgac
     4681 ggcaaactgc cttctgacct ggcaattgcc acctttggcg ataacgaact gctcgacttc
     4741 ttacagtgtc cggtgctggc agtggctcaa cgtcaccgcg atgtcgcaga gcgtgtgctg
     4801 gagattgtcc tggcaagcct ggacgaaccg cgtaagccaa aacctggttt aacgcgcatt
     4861 aaacgtaatc tctatcgccg cggcgtgctc agccgtagct aatccgcgaa caaaaatacg
     4921 cgccaggtga atttccctct ggcgcgtaga gtacgggact ggacat
//
LOCUS       DAZPCS010000214         4873 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZPCS010000214 DAZPCS010000000
VERSION     DAZPCS010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4873
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.217"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(77..838)
                     /gene="kduD"
                     /locus_tag="ACHUQ7_003731"
     CDS             complement(77..838)
                     /gene="kduD"
                     /locus_tag="ACHUQ7_003731"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFR1136940.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(868..1704)
                     /gene="kduI"
                     /locus_tag="ACHUQ7_003732"
     CDS             complement(868..1704)
                     /gene="kduI"
                     /locus_tag="ACHUQ7_003732"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFR1136941.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVGVKD
                     LR"
     gene            complement(1991..3172)
                     /locus_tag="ACHUQ7_003733"
     CDS             complement(1991..3172)
                     /locus_tag="ACHUQ7_003733"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFR1136942.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGEQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            3427..4656
                     /locus_tag="ACHUQ7_003734"
                     /pseudo
     CDS             3427..4656
                     /locus_tag="ACHUQ7_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
BASE COUNT         1283 a         1230 c         1054 g         1306 t
ORIGIN      
        1 atgatatgga ttatgatgat ctagagatat aaaaaaccct gccatgcggc agggtcataa
       61 aagtaagagg aatgaattaa cgcgccagcc aaccgccatc cacagcaatg gtataaccat
      121 ttacataatc tgaagcgctg gaggcaagaa acactaccgg ccccatcagg tcactcggca
      181 gtccccaacg gccagctgga atgcgatcga gaatttccgc gctacgttgt tcatctgccc
      241 gcagttgttg ggtattgttg gtcgccatgt aacccggagc tatcgcatta acattaatgt
      301 tgtgtttagc ccattcgttt gccatcaatc gcgtcacacc catcacgccg ctttttgatg
      361 cggtataaga aggcacacgg atcccgccct ggaaggagag cattgacgcg atattgataa
      421 tcttgccgcc attgccttgc gcgataaagt gtttcgccgc tgcctgagac atgaagaata
      481 cgctcttgat attcaggttc atgacatcgt cccagtcttt ttcgctgaac tcgagagcgt
      541 cttcgcggcg aatcaatccg gcgttattca ccaggatatc aatatgacca aattcagcta
      601 ccgcgcgatc cagcagtcca ggaatgccat caatctttcg cagatcggcg gtcaggctta
      661 aaaaacgacg ccccagcgcc gtgacctgct cgatggtttc agtcggttca acgatgttaa
      721 tgccaacaat gtcacagccc gcttgcgcca gccccaacgc catcccctgg cccagcccag
      781 tatcacaacc agtgacgacc gcaactttac cttggagaga aaatgcactt aaaatcataa
      841 caacacctta ttcgtttatg cccacaacta gcgcaaatct ttaacgccca catggtccat
      901 atcattaaag acctggtttt caccgaccat gccccagata aaggtataag ctttggttcc
      961 gacgccggaa tggatcgacc agctcgggga gatcaccgcc tgctcgttat gcatcacaat
     1021 gtgacgcgtt tcttgcggct gccccatcat gtggaaaacg caggcgtcat catccatatt
     1081 gaaatagaaa taaacttcca tccggcgctc gtgggtgtga cacggcatgg tgttccacaa
     1141 gttacctgga gccagctccg tcagccccat actcaattga caggtttcca gtacatccgg
     1201 gacaaaatat ttgttaatcg tgcgacggtt actggtgagg ttatcgccta acgtgactgg
     1261 agatacttcg tccggtgtga cttttttggt gggatacgtc gtatgcgcgg gtgcgcaatt
     1321 gtaataaaac ttcgccggag tggcggtatc aatactggca aagacaactt cttttgcacc
     1381 tttaccaaca tacagggcgt cgcggtgacc gatttcatag cattgaccat cgacagtaat
     1441 cgtaccggca ccgccaatat tgataacacc taactcgcga cgttcaagga aatagcttac
     1501 gccgagttgt ttaccaactt ccccgccaac ggaaaccgtt ttagttatcg gcataatgcc
     1561 gccaacaata attcggtcaa tgtggctgta aaccatggtg tactcatcgg cgacaaatac
     1621 cttttcaacc aaaaattcat tgcgcagccc ttgggtatcc agcgtttttg cgtgcgcacc
     1681 gtggatgctc tgtcttacgt ccacatcaac ctccgataat cagtgaagcg tgaaaataga
     1741 aaacgatagt gaaacactgt ttcgttttct ctataaaggg atgatatgcc catgggctag
     1801 ggttttcaat taaaaataaa acaatgtttt atttttaatt taaggattaa aaaaagtgca
     1861 gtgtcgatca cgaatatgtc atgagaatgg aaaaatgagg gagcccagaa aatgtatgtc
     1921 gacttgccag ccatcattat tcaacagatt aatgacggca gatatttatc ggtaaggagg
     1981 aaatgcagag ctactcgtca cgttcaatgg tcaatgccac gccctggccc ccgccgatac
     2041 aaagcgttgc cagccctttg cgggcattac gtttcaccat ttcatgaacc agagaaacca
     2101 ggattcggca accggaagcg cctatcgggt gaccgagtgc gatcgcgcca ccattgacat
     2161 tgacccgacg ctcatcccac tcaagcatct tgccaaccga aagcgcctgt gcagcaaacg
     2221 cttcattagc ctcgataaga tcgacatcag ccaactgcca gcctacacgc tccaggcaac
     2281 ggcgggtcgc atacaccggc gcaattccca tcaatgccgg atctacacca acgctggcaa
     2341 atgcgcggat gcgggccagc acgggtaaat tcaacgctcg tgctttggct tcgctcatca
     2401 tcattaccgc agctgcgcca tcgtttatgg atgatgcatt ccccgcggtc acagaaccga
     2461 gactatcaaa tgaaggattt aaacgggcta aaccttctgc actggcgtca gtgcgtggct
     2521 gttcatcggt atcaacaacc aacgtctgac cgttactttg gatcattacc gggacgatct
     2581 catctttaaa tcgtccggcg tcaatcgccg ctcgcgcttt ttgttgcgag ctaagtgcgt
     2641 aagcatcctg caactgacgg ctgatgccat attcgcgagc cagattttcg gcggtgacac
     2701 caatatgata atcattgaag gcatcccaca acccatcatg caccagactg tcaaccaact
     2761 ggctattgcc aagctgttca ccggtgcggc tatcagtcag aacatgtggt gcgcggctca
     2821 tgttttcctg gccacctgcg atgacaatat cagcctcgcc acactgtatc gcctgagtag
     2881 ccagatgcag tgctttaaga ccggaaccgc aaacgtcatt aatcgtgatt gcagaaacgc
     2941 tgttaggaag accaccttta atagccgatt gccttgccgg attctgccct gcccctgcag
     3001 tcaacacctg accaagaatt acttcatcca ccgcatatgc aggaacgccg gtacgttcta
     3061 ttaacgcttt cacgaccaga ctaccaagtt ccacggcgga atgacccgct aacgcaccac
     3121 gaaagcagcc gataggtgtc cgtaacgccc cgacaatcac aacgtctttc atcacaacct
     3181 cgcaccaatt aacagtgcaa tagtaaatga ttgttaatta cagttatctt aattgtttaa
     3241 aaaaagtgat ttttatcaca aaggaaatat gcctgagcag tagtcagagt cataactggc
     3301 acgtaaggtt tgcaaccact aacccaccaa tagatgggta gatagggcgt taatctccca
     3361 tacttaactt ggtttatggt aaattgccct ccattttgtt taatttgtag atgatacgtt
     3421 cagataatgt ctaatatttg gtctaaagaa gaaactctgt gaagtttcgc gctctatggc
     3481 acagccgttg atgcaggcac gctattcctt cctattcagt taggttcggc aggggctgtg
     3541 gtcctgttta ttactgctct ggtcgcctgg cctttaacat attggccaca taaagcctta
     3601 tgccagttca tcctctcatc aaaaacatca gcaggtgaag ggataacggg cgcggtaaca
     3661 cactactatg gcaagaagat tggtaatctg attaccacgc tgtacttcat cgcctttttt
     3721 gtcgtcgtgt tgatatatgc agtggcaatt accaactcac ttacggaaca gctggcaaag
     3781 catatggtta ttgatcttcg catccgtatg ttggtgagtc tgggtgttgt attaattctg
     3841 aatctcattt ttctgatggg acgtcatgcc actattcggg taatgggatt tttggtattc
     3901 ccattgattg cctatttctt atttctttcc atttaccttg tcggtagttg gcaacctgat
     3961 ctattaacaa cccaggtaga gttcaatcag aatacccttc accagatatg gatatcgatt
     4021 cccgtgatgg ttttcgcctt tagccatacg cccattattt ctacgtttgc catagacaga
     4081 cgtgaaaaat atggcgaaca cgctatggat aaatgcaaaa aaattatgaa agtcgcttat
     4141 ctcatcatct gcataagtgt actgttcttt gtctttagct gcctgctttc tattccatct
     4201 tcgtatattg aagcggctaa agaagaaggg gtcaccattt tatcggcgct ttctatgctg
     4261 ccgaacgccc cagcatggtt gtcaatttcc gggattattg tcgcagtagt tgcgatgtcg
     4321 aaatcattcc tgggtacgta ctttggcgtt attgaaggtg ccacagaggt cgtcaaaaca
     4381 acactacagc aggttggtgt aaagaaaagt cgtgcattta accacgcact atcaattatg
     4441 ttggtatcgc tgattacctt cattgtttgt tgcattaacc cgaacgcgat ttcgatgatt
     4501 tacgcgatca gcggcccgct cattgccatg atacttttca tcatgcctac gctgtcaacg
     4561 tatctcatcc cggcgcttaa accctggcgt tccatcggaa atctgattac gctgatcgtg
     4621 ggtatcctgt gcgtatcggt aatgttcttt agctaatatc cgttgtggat ggttcaaaat
     4681 tgctacaatt ttgaaccatt ccatttttca taatattcat ttgttattat tccgccctat
     4741 cacttctctc gtcatatcct agaattatca gttagttaaa cacacaaaga gatctgaaca
     4801 tcttaaataa gactatttaa gatgcataac ttagattcgc aagatatctt ctggtcattc
     4861 aaaaacaatt tgc
//
LOCUS       DAZPCS010000215         4870 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZPCS010000215 DAZPCS010000000
VERSION     DAZPCS010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.218"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..527
                     /gene="ydcF"
                     /locus_tag="ACHUQ7_003735"
     CDS             <1..527
                     /gene="ydcF"
                     /locus_tag="ACHUQ7_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFR1136943.1"
                     /translation="TTGRAEATILVDIAHQFWHIPHEKIWIEDQSTNCGENARFSITL
                     LNQAVERVHTAIVVQDPTMQRRTMATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVI
                     FINQLQGLWPVERYLSLLTGELPRLHDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKH
                     DAVLIEAMESRSLR"
     gene            724..2163
                     /gene="aldA"
                     /locus_tag="ACHUQ7_003736"
     CDS             724..2163
                     /gene="aldA"
                     /locus_tag="ACHUQ7_003736"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFR1136944.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLCKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNLLGEALQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(2205..3205)
                     /gene="gap"
                     /locus_tag="ACHUQ7_003737"
                     /pseudo
     CDS             complement(2205..3205)
                     /gene="gap"
                     /locus_tag="ACHUQ7_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
     gene            3394..3924
                     /gene="cybB"
                     /locus_tag="ACHUQ7_003738"
     CDS             3394..3924
                     /gene="cybB"
                     /locus_tag="ACHUQ7_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFR1136945.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            4169..4342
                     /locus_tag="ACHUQ7_003739"
     CDS             4169..4342
                     /locus_tag="ACHUQ7_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1136946.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            4368..4436
                     /gene="yncP"
                     /locus_tag="ACHUQ7_003740"
     CDS             4368..4436
                     /gene="yncP"
                     /locus_tag="ACHUQ7_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HFR1136947.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(4414..4563)
                     /gene="hokB"
                     /locus_tag="ACHUQ7_003741"
     CDS             complement(4414..4563)
                     /gene="hokB"
                     /locus_tag="ACHUQ7_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFR1136948.1"
                     /translation="MKRNPLVVCLLIICITILAFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(4664..4804)
                     /locus_tag="ACHUQ7_003742"
                     /pseudo
     CDS             complement(4664..4804)
                     /locus_tag="ACHUQ7_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1247 a         1120 c         1208 g         1295 t
ORIGIN      
        1 gcaccactgg cagagcagaa gcgaccatcc tggtggatat cgctcatcag ttctggcata
       61 ttccgcatga aaaaatctgg attgaagacc agtcaacaaa ctgcggtgaa aacgcacgct
      121 ttagcatcac gctattgaat caggccgtag aacgagttca tacggctatc gttgtacagg
      181 accccaccat gcagcggcgc acgatggcaa cgttccgccg tatgaccggg gacaatcccg
      241 atgcaccacg ctggttaagt tatcccggat tcgttcctca gttaggaaat aacgcagaca
      301 gtgtaatctt tattaatcag ttacaaggat tatggccggt tgagcgttat ctctcactac
      361 tcactggcga gctgccgcgt ttacacgatg atagcgatgg ctacggtccc cgcgggcgag
      421 attttatcgt tcacgttgat tttccggcag aagtcatcca tgcatggcaa acgctgaaac
      481 atgatgcggt gctcatcgag gcgatggaaa gtcgctcgtt acgttaaaaa ttgcccgttt
      541 gtgaaccact tgtttgcaaa cgggcatgac tcctgacttt tatttctgcc ttttattcct
      601 tttacacttg tttttatgaa gccctccaca gacttgtcct ttcccgatgc cgtctctctg
      661 atgattgatg ttaattaaca atatattcac cgaaaacaaa catataaatc acaagagtcg
      721 cccatgtcag tacccgttca acatcctatg tatatcgatg gacagtttgt tacctggcgt
      781 ggagacgcat ggattgatgt ggtaaaccct gctacagagg ctgtcatttc ccgcataccc
      841 gatggtcagg ccgaggatgc ccgtaaggca atcgatgcag cagaacgtgc acaaccagaa
      901 tgggaagcgt tgcctgctat tgaacgcgcc agttggttgt gcaaaatctc cgccgggatc
      961 cgcgaacgcg ccagtgaaat cagtgcgctg attgttgaag aagggggcaa gatccagcag
     1021 ctagctgaag tcgaagtggc ttttactgcc gactatatcg attacatggc ggagtgggca
     1081 cggcgttacg agggcgagat tattcaaagc gatcgtccgg gagaaaatat tcttctgttt
     1141 aaacgtgcgc ttggtgtgac taccggcatt ctgccgtgga actttccgtt cttcctcatt
     1201 gcccgcaaaa tggctcccgc tcttttgacc ggtaatacca tcgtcattaa acccagtgaa
     1261 tttacgccaa acaatgcgat tgcattcgcc aaaatcgtcg atgaaatagg ccttccgcgc
     1321 ggcgtgttta accttgtact ggggcgtggt gaaaccgttg ggcaagaact ggcgggtaac
     1381 ccaaaggtcg caatggtcag tatgacaggc agcgtctctg caggtgagaa gatcatggcg
     1441 actgcggcga aaaacatcac caaagtgtgt ctggaattgg gtggtaaagc accggctatc
     1501 gtaatggacg atgccgatct tgaactggca gtcaaagcca tcgttgattc acgcgtcatt
     1561 aatagtgggc aagtgtgtaa ctgtgcagaa cgtgtttatg tacagaaagg catttatgat
     1621 cagttcgtca atctgctggg tgaagcgttg caggcggttc aatttggtaa ccccgctgaa
     1681 cgcaacgaca ttgcgatggg gccgttgatt aacgccgcgg cgctggaaag ggttgagcaa
     1741 aaagtggcgc gcgcagtaga agaaggggcg agagtggcgt tgggtggcaa agcggtagag
     1801 gggaaaggat attattatcc gccgacattg ctgctggatg ttcgccagga aatgtcgatt
     1861 atgcatgagg aaacctttgg cccggtgctg ccagttgtcg catttgacac gctggaagat
     1921 gctatctcaa tggctaatga cagtgattac ggcctgacct catcaatcta tacccaaaat
     1981 ctgaacgtcg cgatgaaagc cattaaaggg ctgaagtttg gtgaaactta catcaaccgt
     2041 gaaaacttcg aagctatgca aggtttccac gccggatggc gtaaatccgg tattggcggc
     2101 gcagatggta aacatggcct gcatgaatat ctgcagaccc aggtggttta tttacagtct
     2161 taatgagtga aagaggcgga ggttttttcc tccgcctgtg cgcgtcagag tttagcgaat
     2221 ttttcgaggg tgcgaataag ctgcgtgacg aagccatatt cgttatcgta ccaggcgacc
     2281 gttttcacca gttgtaaatc gcccacggcg gtaatttccg tttgcgtggc atcaaacact
     2341 gaaccgaaat ggctgccaat gatatcggaa gagactattt cttcatcggt ataaccaaat
     2401 gactcgttat tggtggttgc ttgtttaagt gcgttattca cctcttcggc agtcactttt
     2461 ttccgagaat ggacaccagc tcagtgaccg aacctgtttt caccggcacg cgttgcgcat
     2521 gacctttcag tttgccgctc agttccggga tcaccagacc aatggctttt gccgcccccg
     2581 tagtgtgggg aatgatattt tctgccgctg cgcgtgaagc acgtaaatct ttaccacgcg
     2641 ggccatccac cagtgactgg gtgccagtat aggcatgaat ggtcgtcatc gtgccgactt
     2701 ctatcccgaa actgtcatgc aaggctttgg ccatcggcgc aaggcagtta gtggtgcatg
     2761 acgccacgga aacaatggtg tcgttgccat ccagagtgtc gtcattaacg ttataaacga
     2821 tggttttcat ttcaccggca ggagcggaaa tcaacacctt cttcgcacca gcatcaagat
     2881 gcgcctgcga tttatcggcg gaggtataaa agccagtaca ttcgacaatg atttctgccc
     2941 ctttcgcttt ccacggaata tttttagcct ctttttcggc gtaaaccgcg atacttttcc
     3001 catcaacgat aagtgaatct tccgtaaaat caacgctcca ggggaacgga ccatagtttg
     3061 aatcatgttt cagcagatag gcgagaattt ttggggaagt aagatcatta atagcgacaa
     3121 cgtctatgtt gcttttgact tcaagtaaac gacgcaacac cagtcgaccg atacgaccaa
     3181 aaccgttaat accaacttta ctcatggttt tctcctgtca ggaacgttcg gatgaaaatt
     3241 gatcctttcc aggcttagac caggattgtg ggatgggcaa tctccattct tacagtgaaa
     3301 cgtaacgtac tgaaaacggg tgaacaatat ttaatgaaat tttgagaaaa gcccgttatg
     3361 ttaacggaaa attatgttaa agcaggaaat gttatggaaa ataaatactc aaggttacaa
     3421 atcagcattc actggctggt ctttttgctg gttatcgcag cgtattgcgc aatggagttt
     3481 cgtggtttct tcccacgtag cgatcggccg ctcatcaaca tgattcatgt ttcctgtggc
     3541 atctcaattc tcgtgctgat ggtggttcgt cttttgttaa gactgaaata cccaaccccg
     3601 ccgattatac ctaagccgaa accgatgatg acgggactgg cgcatttggg acatttggtg
     3661 atttatctcc tgtttattgc gctgccagtg attggtttgg tgatgatgta taaccggggc
     3721 aacccgtggt ttgcgtttgg tttgacgatg ccttacgctt cagaggccaa tttcgaacgg
     3781 gttgatagct taaagtcgtg gcatgagacg ctggcgaatc tgggatattt tgtcatcggg
     3841 ttgcacgctg cggctgcact ggcacaccac tatttctgga aggacaacac acttctacgc
     3901 atgatgccgc gtaaacgttc ctgaaggata tttaaagaaa acgcctgtac taaaaccgac
     3961 ccgtggtaca ggcgaagaat acgggtctac atcggaagcg cctattatat ttatttgtat
     4021 gacaaataaa accataatcc ttgcccatac gtccatctgg cttattttta atcaattcac
     4081 ccgatctttg atctcatcaa cggtatcaaa ataaaccagc gtataacgtt caaatcgcac
     4141 ataagattcg ataaaccatg gaggttatat gaaaaaactg gcacttattt tgtttatggg
     4201 aacgcttgtt tccttttatg ccgatgccgg gcgcaaaccc tgttctggtt cgaaaggggg
     4261 gatctcacac tgtacggcag gcggcaaatt tgtctgtaat gatggttcta ttagtgcatc
     4321 gaaaaaaaca tgcactaact gaagtgtaaa aggggtgcca tgagaaaatg aatctgctag
     4381 tcagatgcgc ggggaaaatc cccgcgcttg cccttacctg gacgtgcagg ccatgagcgc
     4441 agcaacctcc ttatcaccgt cccggaaccg cagttcgtag agcgtttgtc gggtcaggag
     4501 tgtgaatgcc agaatcgtaa tgcagataat gagcagacac accaccagag ggttgcgctt
     4561 catagccttt ctccttgccg gatggcgggt aagaggctaa caatctgaat tgcttggttc
     4621 attcgttggc ctcggtttgt actgacccca aaaagttgga cagttaaaca cgaggcatat
     4681 aggtctgatt ccgatattca tttggagtca gaccttttaa tttcaggcta attcttctgc
     4741 tgttgtagta ttcaatatat tccgtaacag catccttcag ttcgcttata ttactgaact
     4801 cattagactg gccccctgaa tctccagaca accagtatca cttatttaag tgatagtctt
     4861 aatactagtt
//
LOCUS       DAZPCS010000216         4867 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZPCS010000216 DAZPCS010000000
VERSION     DAZPCS010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4867
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.219"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..309)
                     /gene="gadE"
                     /locus_tag="ACHUQ7_003743"
     CDS             complement(<1..309)
                     /gene="gadE"
                     /locus_tag="ACHUQ7_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFR1136949.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     Y"
     gene            complement(1108..1680)
                     /gene="hdeD"
                     /locus_tag="ACHUQ7_003744"
     CDS             complement(1108..1680)
                     /gene="hdeD"
                     /locus_tag="ACHUQ7_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFR1136950.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            1935..2267
                     /gene="hdeA"
                     /locus_tag="ACHUQ7_003745"
     CDS             1935..2267
                     /gene="hdeA"
                     /locus_tag="ACHUQ7_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFR1136951.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            2383..2709
                     /gene="hdeB"
                     /locus_tag="ACHUQ7_003746"
     CDS             2383..2709
                     /gene="hdeB"
                     /locus_tag="ACHUQ7_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFR1136952.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            2773..3419
                     /locus_tag="ACHUQ7_003747"
                     /pseudo
     CDS             2773..3419
                     /locus_tag="ACHUQ7_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(3461..3991)
                     /locus_tag="ACHUQ7_003748"
     CDS             complement(3461..3991)
                     /locus_tag="ACHUQ7_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFR1136953.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(4147..4713)
                     /gene="slp"
                     /locus_tag="ACHUQ7_003749"
     CDS             complement(4147..4713)
                     /gene="slp"
                     /locus_tag="ACHUQ7_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFR1136954.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT         1491 a          978 c          992 g         1406 t
ORIGIN      
        1 ataagtaatc tcaagaagat ttttgatact ttctttgcgg ctaacaaaat aaataggcgc
       61 tttagctttt agtttactga tgtgataagg tgccaaaaca ataattctct cggcatctaa
      121 tttctccaga aatttaatcg cttcttcatc aaggatatga ttgtgatagg tatcaatgat
      181 aaccttgaag ggtttattgc ctactttttt gatctctgac agggaattga tttttatcat
      241 ttcgtgatta tctttcaact gccaaaagcc ctgtaaaaga aaagaatctt tcgtcatgag
      301 aaaaatcata acttgctcct tagccgttat cgtttcgaac atgtcatcct tttcattatt
      361 tacatccttg tccgaatcgt tgttcaatat agtaaacgcc tataagaact ttgcccttcg
      421 tgaactaata cgtctattaa cactaatcaa tacttcagat ttatccatca cgcactccat
      481 atattgatcc taagtaagat tcctggttgt tatcagcttg taaatagtaa acaaaaagtt
      541 aacgtttgcg gattactgcc caagaataag ttatcaataa ccagtttgtg atctctgaag
      601 aatattacta aagttaaaat gtaatccgat ttaaatatcg agtctccttg tttcgactta
      661 agctggcaat tggattgcca gctttctttt aacgcattga aattacctga gtaaataacg
      721 cattcatgtc ttcaggtttt tatatagcgt ctggagacac ggatatttat gcaatgtaat
      781 caacgccagt tgcaaaaaaa tgaacccgga ttacttattt aaccgcccat agcatcaacg
      841 cttacggaac tctttgttca ctttcccaca gcacttttaa aacattaaac ctgactacgg
      901 aaaatatcag ccatgataat gtttgaaatg gctaatttgc catggagtga aaaaaattag
      961 atgaaattca gtaggttgaa ataatcacta gcaggtaatt atttcaatga tagtgcgcaa
     1021 ttgatctaca acactgcgta gcggagagag tattaatcgg atcatagtca catcaagtga
     1081 ctatgatccg ggtgacaacc ggggtaatta ttgctgctta acgaacaaac tggcgaagct
     1141 gaacaggctg gcggcgctaa atatcagttc aattcccacc agtgtggaaa ccagcgttac
     1201 agacaccatc ggcgttgcac caaggaatat ccaggcaatg acgatatcca gcacaccaat
     1261 aacgagctgt agccagctgc ctttcattga acgctgacga taccaactca tcaggcgaat
     1321 aacccctgca acacagaaca aaccggcaat aaatgccgca atggcaaaaa tgcccagctc
     1381 cggtgcacgg atgaagaaat agccgatcaa taaataggcg actgcgacga ggaaaccgga
     1441 taataccggc cagaaattat gactgcggtt gctgaataac ccgacaataa gcgcaatacc
     1501 cgagcagatt aataatgcac ccactactgt gcttaaaata tcgccagaga cgaacgggaa
     1561 actgatacac agcaacccga cgataaacag cagcacggca ataaactgga ttgctctgcg
     1621 atgctttttc agcatctcca gatcaaactt caaaattgtt gccttatcta tatataacat
     1681 agaaccaccc tataaaatta agaagaaaat cccctgctat caatctatgc caaaaacgcg
     1741 tctaagaatg cagtcgattt aataaaaatt tcctaattgc agtatctgat gcatctgtaa
     1801 ctcattgtat tgaaataaaa atatctgatt ttgatatttt ccatcaacat gacatataca
     1861 gaaaaccagg ttataacctc agtgtcgaaa ttgattcgtg acggctcttt cactttatag
     1921 ttgaggatat tacgatgaaa aaagtattag gcgttattct tggtggtctg cttcttctgc
     1981 cagttgtgag caatgcagcg gatgcgcaaa aagcagctga taacaaaaaa ccggtcaact
     2041 catggacctg tgaagatttc ctggctgtgg acgaatcctt ccagccaact gcagttggtt
     2101 ttgctgaagc gctgaacaac aaagataaac cagaagatgc ggttttagat gttcagggta
     2161 ttgcaaccgt aaccccagct atcgttcagg cttgtactca ggataaacaa gccaacttta
     2221 aagataaagt taaaggcgaa tgggacaaaa ttaagaaaga tatgtaattc cgggaatgcg
     2281 ttacatcgta cttccttgca tattgaacag gccggaatat cttctttaaa agcagctatt
     2341 cctcctgttc atatataatc tctatattga atgggttaca aaatgaatat ttcatctctc
     2401 cgtaaagcgt ttatttttat gggcgctgta gcggctttgt cactggtgaa cgcacaatct
     2461 gcgttggcag ccaatgaatc tgctaaagat atgacctgcc aggaatttat tgatctgaat
     2521 ccaaaagcaa tgaccccggt tgcatggtgg atgctgcatg aagaaacagt atataaaggt
     2581 ggcgataccg ttactttaaa tgaaaccgat ctcactcaaa ttcctaaagt gatcgaatac
     2641 tgtaagaaaa acccgcagaa aaatttgtat accttcaaaa atcaagcatc taatgacttg
     2701 ccgaattaat gaggtacaag taaaaaggag tagcaagttg agccatcttg ctgctccttt
     2761 ttgcattttt atatgacagc agaatttatt atacgtctta tactcgcggc aattgcctgt
     2821 ggcgctattg gcatggaaag gcaaatgcgc ggcaaaggag cagggttacg cacacatgta
     2881 ttaattggca tgggaagcgc cctgtttatg attgtttcga aatatggttt tgctgacgtg
     2941 ctgtctttag atcacgtcgg actcgacccc agccgtattg ctgctcaggt ggtgacgggc
     3001 gtcgggttta tcggtgcagg taacattctc gttcgtaacc aaaatattgt aggtctgacg
     3061 acggcagcgg atatctgggt gaccgccgca taggtatggt tattggcagc ggtatgtacg
     3121 aactgggtat ttatggttca gtgatgacct tgttggtgct ggaagtcttc catcaattaa
     3181 ccttccgcct gatgaacaaa aattaccatt tacagctgac attagtgaac ggtaatacgg
     3241 tatcaatgct cgactggttt aagcagcaaa aaataaaaac tgatttagtt tcgttgcagg
     3301 aaaatgaaga tcatgaagtg gtcgccattg atatccagct tcatgccaca acgttgatag
     3361 aagacctgtt gcgattatta aaaggtatgg caggggtgaa aggcgtttca attagctaat
     3421 ctgtgtggca gcgtagccag actcaccgta agcctgaaat tcacaccaga taatcaatat
     3481 gctgatggcg taccagctca ttgatccgct taagattgag gatcatcatg atattgtgtt
     3541 tatggcagta gaacgttttt agcgatattt tcagtttctc cagaatatct tcagttgtca
     3601 tttgattcgc aatgcagctc agaacctgat gttgcttgcg cgaaagtgac acattcgctt
     3661 caggcacaga gcagtaactg ccattgatga gtgcgacaaa atcaatgata gtgatagtcc
     3721 gcggaacaaa ggtcaccggt actttcccca gacagcgttt aatattaaag ggggagaaaa
     3781 caatgacatg gacaggtttt aatcgttcaa tttgagtgag aaaattagaa tgaaatacat
     3841 tattcattaa tgtatcaata atgacgaagg cattctgatg caacattacg tcgatctctt
     3901 cttcgttctg tatatgaacg acattacctt tactcagaat gtttttcatc gcggtgaaga
     3961 acatcgtatc cctggtaatt ataagaaaca taaccgactc ctggtgcgga cattaacttt
     4021 ataatcagtt tgccaaaggt aattaacagg acagggctta taataataat agttagctta
     4081 ctgtatatta attttatgta atacatgatg cagatgctga gaaagcatct gcatctttcg
     4141 gtggtgttat ttgaccagct caggtgttac ctgactcacc gcattggtgt agtaaggcgc
     4201 accccagccc ggttccggcc agaatgcacc atagccgtaa tcccacaggt tataggtggt
     4261 attaaccact tctctcaaat gccacacctg gatgccctgc atattcactt ccaggaagtt
     4321 atacgggact ttattgataa agcctggttg ttcaccctga atggtaccga ggatggtaac
     4381 aaagtgatta cgatagttca ctggatcaag gaagccgctt tgtctggcga gcagtcggcc
     4441 ctgatagttg gcttcaatat caggcttcgc atagctatcc aacggtaata cagcgatttc
     4501 taacaacgta tccgttttgc cattgataac gttgataacc ttcccaccaa agcgcgcttg
     4561 ttgaccaaca tataaccccg gctggttatg aacagcaaca aaactttttt gaatatcagg
     4621 ttggttattg cctttgatat tttgcggaat tgaactacat gcggcaagca aaaatgaaag
     4681 gctgaggatg agtgcacctt ttgtcatgtt catgttacta tccttatcaa caaatattaa
     4741 aaccataata gatatttctt tatagttttc atctgattct gaggtgatat gcctgatagt
     4801 aacgtaaacc tgtctgatgt ttatatccct tctgtttagt tcaattccgt tattttgggg
     4861 cgtggta
//
LOCUS       DAZPCS010000217         4833 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZPCS010000217 DAZPCS010000000
VERSION     DAZPCS010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4833
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.220"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(38..414)
                     /gene="rnpB"
                     /locus_tag="ACHUQ7_003750"
     ncRNA           complement(38..414)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHUQ7_003750"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(447..1592)
                     /gene="garK"
                     /locus_tag="ACHUQ7_003751"
     CDS             complement(447..1592)
                     /gene="garK"
                     /locus_tag="ACHUQ7_003751"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFR1136955.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNTG"
     gene            complement(1689..2579)
                     /gene="garR"
                     /locus_tag="ACHUQ7_003752"
     CDS             complement(1689..2579)
                     /gene="garR"
                     /locus_tag="ACHUQ7_003752"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFR1136956.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALVCYYEKLAKVEVTR"
     gene            complement(2609..3379)
                     /gene="garL"
                     /locus_tag="ACHUQ7_003753"
                     /pseudo
     CDS             complement(2609..3379)
                     /gene="garL"
                     /locus_tag="ACHUQ7_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
     gene            complement(3395..4729)
                     /gene="garP"
                     /locus_tag="ACHUQ7_003754"
     CDS             complement(3395..4729)
                     /gene="garP"
                     /locus_tag="ACHUQ7_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFR1136957.1"
                     /translation="MILDTVDEKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVICFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
BASE COUNT         1160 a         1383 c         1162 g         1128 t
ORIGIN      
        1 aagcaaaaac ccgccgaagc gggtttttac gtaaaacagg tgaaactgac cgataagccg
       61 ggttctgtcg tggacagtca ttcatctagg ccagcaatcg ctcactggct caagcagcct
      121 acccgggttc agtacgggcc gtaccttatg aacccctatt tggccttgct ccgggtggag
      181 tttaccgtgc cacggactgt taccagccgc gcggtgcgct cttaccgcac cctttcaccc
      241 ttacctgatc ccgcttgcac gggccatcgg cggtttgctc tctgttgcac tggtcgtggg
      301 tttccccccc aggcgttacc tggcaccctg ccctatggag cccggacttt cctcccctcc
      361 acccgtctcc cccgaagagg acgacgacga agcggcgact gtctggtcag cttcggcgcg
      421 cagtatagag ggtttgcgcg cccttgtcac cccgtgttgc gcattccaat cgccagtgtc
      481 gcggcaatat tacgtgaagc acggcagata ttgtcataag ccccgcggaa tgcttcgtcc
      541 aacgtaccta tgctggtcaa tacgctgaag accgcatcaa tgccatgctg atgtacaacg
      601 ccaacatcat cggtcaggct acccgcaatg ccaatcaccg gtttatggta cttcttcgcc
      661 acgtttgcga cgccaatcgg taccttgccg tgaatactct ggctgtcaat acgcccttca
      721 ccggtgatca ccagcgtaca atcgtgaata tgttcctcca gattcagcgc cgtagtgacg
      781 atttcaatac cacttttcag ttccgcacca agaaacgcca tcagcgccgc acccataccg
      841 cccgcagctc ctgcgccggg gacatctttt acatcaacat gcagcgcttt tttaatgaca
      901 tcggcatagt gagagaggtt attgtccagc tcaacaatca tcgcttcact ggctcccttt
      961 tgtgggccaa agatgcgcga tgcgccgtta tcgcccacca gcggattggt gacatcacaa
     1021 gcgacgcgaa tgacgcaatc ttttaagcgc ggatcgaggc cggaaatatc aatatcattc
     1081 agagtattaa gactaccgcc gccaaaacca atttcattgc cgttggcgtc gcataatttc
     1141 gcccccagcg cctgtaccat gcctgcgccg ccatcatttg tagcgctacc gccaatgccg
     1201 ataataatgt ttgtcgcgcc gctctccagc gcctgcagga ttaactcgcc tgtgccgcgt
     1261 gaagtggtca cgagtggatc gcgtttttcc gcaggtacca gctccagccc actggccgcc
     1321 gccatttcaa taaacgcggt tttgccatcg ccggagatcc cccaactggc attcactttc
     1381 tcgcccagcg gccctgtaac ccaggcgtga cgttcagaac cctgggtggc tgcaatcatc
     1441 gcttccaccg ttccttcgcc accgtcggca accggaatag aaacgtactg tgcatcagga
     1501 aaaatttccc gaaatccttt ttctatcgcc tgcgcaacct cgctggcaga taaactttct
     1561 ttataagagt ctggggcgat tacgattttc atacctatgc ctgttaccac atgacgccgg
     1621 agggcgtttc tcttattcgg cctggattcc aggcccggat tgcaatacgc catccgggca
     1681 cgacgtcatt aacgagtaac ttcgactttc gccagttttt cgtagtagca caccagggcg
     1741 ctatgatccg ccgttcctaa accatctgct cgcagtgcct gcatcatctc cataaccgca
     1801 gctgtgagcg gcagttgcgc gccgacgccg tgggaagtat ccagcgcatt tgccagatcc
     1861 ttaatatgca gatcaatacg gaagcccggc ttgaagttgc ggtccatcac catcggcgct
     1921 ttggcatcca gcacggtact gcccgccagt ccaccgcgaa ttgcctgata caccaggtcc
     1981 gggttaacgc ccgctttagt tgccagcgtt aacgcttctg acatcgcggc aatattcagc
     2041 gccacaatga cctgattcgc cagtttggtg acgttacctg caccaattgc cccggtatgc
     2101 accacggaac ccgccattgc tttcatcaaa tcatagtatt tgtcgaaaat agccttgtcg
     2161 ccgcccacca tcactgacag cgtaccgtcg atggctttcg gttcaccgcc gctcaccgga
     2221 gcatccagca tatcaatgcc tttcgctttc agcgcttcgc tgatttcacg gcttgccagc
     2281 ggtgcgatag aactcatatc gatcaatacc gtacctggct tcgcgccttc aataatgcca
     2341 ttctcaccca gcgccacctc tttcacatga ggggagtttg gcagcatggt tatgatgacg
     2401 tcgcactgtt cagcgatcgc tttagccgta gaggctgttt ctgcacctgc agcaatcacg
     2461 tcagcaatag cttctgggtt acggtcagca accaccagcg agtaacctgc tttcagaagg
     2521 tttttactca ttggtttacc cataatcccc aggccaataa aaccaacttt catagtcata
     2581 tcaatcatct ctcttgttgc ggtggtggtt attttttaaa ggtatcagcc agtttatgag
     2641 tggcagagcg gaagacgccg agatcgctgc cgacagccac aaacgtcgcg ccccattcca
     2701 gataacgacg cgcatcggct tcgaccggcg cgaggatacc gctgggtttg ccgtgcgcgc
     2761 tggcacggtt aaaaatgtgc tgaattgctt tttgtacatc cgggtgtgat gcattgccga
     2821 gatggcctaa tgccgcggcc agatcgctgg ggccgacgaa gatgccgtct acgccttcgg
     2881 tagcggcaat ggcatcgacg ttatctacgc cctgctgact ttctatctgg accagaatag
     2941 tgatgttctt gttcgactga gcgaaataat ccgccacggt gccaaacata ttggcgcggt
     3001 gagaaacgga gacgccgcga atgccttccg gtgggtaacg ggttgatgcc accgcctgct
     3061 ctgcttcctc ttttgtttct acaaaaggaa tcaggaagtt atagaaaccg atatccagaa
     3121 gacgcttaat aatttccggc tcgttggtcg gcactcacac tactggcgcg ctggcgctgc
     3181 ctttcaaggc cattaactgc ggaataaacg tggagatatc gtttggcgca tgttcgccat
     3241 ccagcaccag ccagtcaaac ccagccaaac caagaacttc agtgctaatc gggttagaga
     3301 gtgctgacca gcaaccaatt tgtacctgtt tcgcagccag tgcggctttg aatttattcg
     3361 ggaaaacatc gttattcatc gcttatacct ttgcttattt ctgcaattcc atacgtttaa
     3421 tgtcgccaac tacgaagagg tagcagacca tcgccatcag cgctgaacat cccacgaaaa
     3481 ccagtgctgc attgaaggag tgcagttcac ttaccaggta gccaatcacc agtggagtga
     3541 caatggaggc aacattgcca aagacgttaa agacgccgcc acagaggcca acaatctctt
     3601 tcggcgcggt gtcagaaatc accggccagc ccagagcacc aaatcctttg ccaaaaaaag
     3661 ccagcgccat cagcatgacc accagcgtgg tgttgttggt gtagttacat aagatgatgg
     3721 tggaagccag caacattccc agcacaatcg gtagcttacg tgccagggtc agggataaac
     3781 cgcgtttgat cagataatcc gagaagacac ctcccagcac gccgcccgca aaaccacaca
     3841 gtgccggaat cgaggcgacc agacccactt tcagaatcga catgcctttt tcctgcacca
     3901 gataaatcgg gaaccaggtg aggaagaacc aggtgatggt gttgataaaa tattgtccga
     3961 aaaatacgcc caacatcatg cggttagaga gcaattgctt gatgtaatgc agtttgggac
     4021 cgcttgctgc cgcgctgccc ggctttttgt ggtccatatc gaccaccgcg ccattttcag
     4081 agataaactt cagctcttcc tcagacatac gtgggtgatc tgtcgggtta tgaatcaact
     4141 tgatccacag cgccgtcagc acaaaacaaa tcacccccat aacggtaaag acgtgctccc
     4201 atccccaggc gaaagtcagc cagccaagca gaggcgaaaa gagcgccagc gagaaatatt
     4261 gtgccgagtt aaagatggcg gaggcagtac cacgttcttt cgtcgggaac caggcggcga
     4321 caatacgggc gttcgccggg aaggatggag cttccgagaa tcccagcata aagcgcataa
     4381 agaacatgga gatccctgcc caagccagcg ggaacatatc aacaaagcct tgcaggaagg
     4441 tgaacagcga ccagaaaaag aggctgtagg tgtaaacttt tttcgagcca aacttatcaa
     4501 gcaaccagcc gccggggatt tgcatcagca agtaggccca gccaaaagcg gagaagatgt
     4561 aacccatcga aaccgcactt aactgcaact cttttgccac ttcggtacca gcaatagaca
     4621 gcgttgcacg atcggcgtag ttaacggcgg taacaataaa aataatcagt aatattaaat
     4681 agcgggtatg cacacctttc cttttttcgt caacggtgtc cagaatcatt ttatttacct
     4741 cgggtactta tgctgatttt tattattatg gggaaggtgt tatttataag tttcatttat
     4801 gccgtaacga caatgaactc gggaattagt ata
//
LOCUS       DAZPCS010000218         4688 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZPCS010000218 DAZPCS010000000
VERSION     DAZPCS010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4688
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.221"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            240..2672
                     /gene="hsdR"
                     /locus_tag="ACHUQ7_003755"
     CDS             240..2672
                     /gene="hsdR"
                     /locus_tag="ACHUQ7_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EcoAI/FtnUII family type I restriction enzme
                     subunit R"
                     /protein_id="HFR1136958.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPQQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGPFPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVHKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2739..4208
                     /locus_tag="ACHUQ7_003756"
     CDS             2739..4208
                     /locus_tag="ACHUQ7_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFR1136959.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4208..>4688
                     /locus_tag="ACHUQ7_003757"
     CDS             4208..>4688
                     /locus_tag="ACHUQ7_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFR1136960.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNN
                     "
BASE COUNT         1357 a         1150 c         1133 g         1048 t
ORIGIN      
        1 tttcttgttc gaaagaaacg caactataag atttttatat attttgttag agttgatatg
       61 gttcctgtaa cttctgttta tctgtctctg atatgcccag attaattcaa gcaagatata
      121 acagagatat caatgtgatc tctgctacgt actttacgtt gaaacaccgc cctaaatcat
      181 agccaggcta ttgattttat gtacaatcag aatcgtcctg ataaaagcga gaacccccca
      241 tggcggaact gaacctaagt aacctgacgg aagcagacat cattaccaaa tgcgttatgc
      301 cagccattct caatgcaggc tgggacaaca caacgcaaat cagacaggag gtcaaactcc
      361 gggacggtaa agtcatcgta cgtggtaaag ttgcggcacg cagaacggta aaatctgctg
      421 acatcgtgct gtaccacaag ccaggcattc ccttagccgt gattgaagca aaagccaaca
      481 aacatgaaat tggcaaaggg atgcaacagg gcattgaata tgcgcgcctg ctggacgttc
      541 cttttgtttt tgccaccaac ggtgatggct ttatcttccg cgatgccacc gcagccgaag
      601 gtgaatgcct tgaaaagcaa ataacgctgg atgacttccc ctcccccgct gaactctggc
      661 aaaaattctg cctctggaaa ggatacacac aagctcagct tccggtgatt actcaggact
      721 attacgacga tggcagcggc aaatcgcctc gttattatca acttcaggca atcaacaaaa
      781 ccattgaagc cgtctccaac gggcaaaacc gcgttctgct ggtcatggcg accggaacag
      841 ggaaaaccta taccgcattc cagatcatct ggcgcctgtg gaaatcaaaa aataaaaaac
      901 gcattttgtt ccttgccgat cgcaacattc tggtcgacca aaccaaaaat aatgatttcc
      961 agccatttgg tacggcaatg accaaagtca gcggacgcac cattgatccc gcttatgaaa
     1021 ttcacctcgc gctctatcag gctataactg gcccggagga agaccaaaaa gcgtttaaac
     1081 aagtcgcacc agatttcttc gatctgatcg tgatcgacga atgccatcgc ggcagcgcat
     1141 ccgaagacag cgcctggcga gagatccttg attatttcag ttccgccacc caaattggct
     1201 taaccgccac gccaaaagag acgcatgaag tctccagcac ggattacttc ggcgatccgg
     1261 tttacgtcta ctcactaaaa gaagggatcg aagacggctt cctcgctcct tataaagttg
     1321 tccgtgttga tattgatgtc gacctgcaag gctggcgccc aaccaaaggg caaactgact
     1381 taaacggcga agtgattgac gatcgtatct ataaccagaa agatttcgat cgcacgatgg
     1441 tgatcgacga acgcacagaa ctggttgcca gaaccattac cgactacctc aagcgcacca
     1501 atccgatgga taaaaccatc gtcttctgta acgacatcga tcatgcagaa cgtatgcgcc
     1561 gcgccctggt taatctcaac ccgcagcagg tgaaaaagaa cgacaaatac gtcatgaaaa
     1621 tcaccggcga tgatgaaatt ggcaaagctc agttggataa cttcatcaac ccgaaaaaac
     1681 cgtacccggt tatcgcgacc acttcagagc tgatgaccac tggtgtggat gctaaaacct
     1741 gcaaactggt agtactagac cagaacatcc agtcgatgac caaattcaag caaattatcg
     1801 gtcgtggtac acgcatcgac gaacgttacg gcaaactctg gtttaccatc ctcgacttta
     1861 aaaaagccac cgaactgttt gccgatgagc gtttcgatgg cattcccgaa aaagtcatgg
     1921 ataccacacc agaggatatc gccgatccag aatctgattt tgaagagaaa ctcgaagaaa
     1981 tcagcgaaca tgacgacgaa caggtaacag gcgttgatga accgcctgcg ccaccatacc
     2041 aggttaccga taccgatgat gtcggcccat ttccggaaga agacgagaag aaaatccgca
     2101 agtttcacgt caacggtgta gcggtgggcg ttattgcaca gcgcgttcag tattacgacg
     2161 ccgacggtaa actggttacc gaatccttta aagattacac ccgcaaaaca ctgctcaaag
     2221 aatatgcctc gctggatgac tttacccgca agtggcagga cgccgatcgc aaagaagcga
     2281 tcattcacga gctggagcaa caggggatca tctgggaagt actggcagaa gaagtcggta
     2341 aagatctcga cccgttcgac atgctttgcc acgtagtgta tggtcagccg ccgttaaccc
     2401 gcaaagagcg cgccgagaac gtgcacaagc ggaactactt cacaaaatac tctgaagcag
     2461 cgcaagccgt gctcgataat ctgctggata aatacgccga tgcgggcgta caggagatcg
     2521 aaagtattca ggtgctgaaa cttaaaccat tcgacagcat gggcacctta ccggagatta
     2581 ttaaaaccgg atttggcgac cgtaacgggt ataatcaggc gctcagcgag ctggaaaacg
     2641 aaatctacca attaccgccc cgctctgctt aacaactctc ttttgcgcct tctgcaacca
     2701 tcatgttgca gaaggccttg tttttcatgg aatacaacat gtctatcagc tcagtaatca
     2761 aatcattaca agatattatg cgcaaagatg ccggtgtgga cggcgatgcg cagcgtctcg
     2821 gtcagctctc ctggctgctg tttttgaaaa tcttcgatgc ccaggaagag gcgctggaac
     2881 tggagcagga taactatcaa tatccgatcc cacagcgtta tttgtggcgc agttgggccg
     2941 ccaacgcgca gggcattacc ggtgattccc tgctggaatt cgttaatgat gatctgtttc
     3001 cggcgttgaa aaacctcact gcgcctatcg ataaaaaccc acgcggctac gtggtgaagc
     3061 aggcgttcag cgatgcctat aactatatga aaaacggtac gctactgcgc caggtgatca
     3121 acaagctgaa cgaaattgac tttaccagcg ccagcgaacg ccatctgttt ggtgatattt
     3181 acgaacagat ccttaaagat ctgcaatctg cgggcaatgc gggcgaattc tatactccac
     3241 gcgccgtcac tcgctttatg gtggatcgcg ttgatccgaa actcggcgaa tccattatgg
     3301 acccggcctg cggtacgggc ggttttcttg cctgcgcgtt cgatcatgta aagaacaaat
     3361 acgtgaagag cgtcgccgat catcagacac tgcaacaaca gatccacggt gttgagaaaa
     3421 aacagcttcc gcacctgctg gcgaccacca atatgctgct gcacggcatt gaagtaccgg
     3481 tacaaattcg tcacgacaac accctgaata aaccgctttc ctcctgggat gagcaactgg
     3541 atgtcattgt taccaacccg ccgtttggtg gcacggaaga agacggtatt gagaagaact
     3601 ttccggcaga gatgcaaacc cgcgaaacgg cggatctgtt tctgcaactg attgtggaag
     3661 tactggcgaa aaacggtcgt gcggcggtgg tattgccgga tggcacacta tttggcgaag
     3721 gtgttaaaac caaaatcaaa aagctactta ccgaagagtg caacctgcat accatcgtgc
     3781 gtttaccgaa tggtgtgttt aatccctata ccggcattaa aaccaacctg ttgttcttta
     3841 ccaaaggtca gccaaccaaa gagatttggt tctatgaaca tccgtatccg gcgggcgtga
     3901 aaaactacag caaaaccaag ccgatgaagt ttgaagagtt tcaggcggag atcgactggt
     3961 ggggtaacga ggccgatggt tttgccagcc gcgtcgagaa tgagcaagcg tggaaagtca
     4021 gcattgatga cgtgattgcg cgcaacttca atctggatat taaaaaccca catcaggcgg
     4081 aaaccgtcag ccatgatccg gacgaactgt tagcgcagta tgcaaaacag caggcggaga
     4141 tccagacgct gcgtaatcaa ctgcgcgata ttcttggcgc tgcgctgtct ggcaaggagg
     4201 ttaactaatg agtgtggaaa agctgatcgt tgatcatatg gaaacctgga cctcggcgtt
     4261 gcaaacccgt tccaccgccg ggcgcggcag ctccggtaag attgatttgt atggtattaa
     4321 gaaattgcgt gagctgattc tggaactggc ggtgcgcggt aaactggtgc cgcaggatcc
     4381 gaatgatgaa ccggcgtcgg acctgctgaa gcgtattgcg gcggaaaaag cagagctggt
     4441 gaagcaaggc aaaattaaaa agcaaaaacc actgccagaa attagtgagg aagagaagcc
     4501 gtttgaattg ccggagggat gggagtgggt gcacctccct gatatttatt gctcaatttc
     4561 tgagtcatct agaaaaataa aatcctccga aatactacct gagggaaaat atcctgttat
     4621 cgagcaaagc caagaattta tttctggtta ttgcaacaat gaatgtctgt taataaagtt
     4681 aaataatc
//
LOCUS       DAZPCS010000219         4603 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZPCS010000219 DAZPCS010000000
VERSION     DAZPCS010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4603
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.222"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(79..867)
                     /gene="phoH"
                     /locus_tag="ACHUQ7_003758"
     CDS             complement(79..867)
                     /gene="phoH"
                     /locus_tag="ACHUQ7_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFR1136961.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLQAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDLLVRRLGASFMQYCLRPEIG
                     KVETAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(1474..2739)
                     /gene="efeB"
                     /locus_tag="ACHUQ7_003759"
     CDS             complement(1474..2739)
                     /gene="efeB"
                     /locus_tag="ACHUQ7_003759"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFR1136962.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALAGSCPVAHAQKTQSAPGTLS
                     PDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLSQG
                     GAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKMTR
                     FPNDSLDAALCHGDVLLQICANTQDTVNHALRDIIKHTPDLLSVRWKREGFISDHAAR
                     SKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQFR
                     VEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLANPR
                     TAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALEEY
                     VKPIGGGYFFSLPGVKDANDYLGRALLQV"
     gene            complement(2745..3872)
                     /gene="efeO"
                     /locus_tag="ACHUQ7_003760"
     CDS             complement(2745..3872)
                     /gene="efeO"
                     /locus_tag="ACHUQ7_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFR1136963.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(3930..>4603)
                     /gene="efeU"
                     /locus_tag="ACHUQ7_003761"
     CDS             complement(3930..>4603)
                     /gene="efeU"
                     /locus_tag="ACHUQ7_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFR1136964.1"
                     /translation="IFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNV
                     KVQLEQAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAM
                     LGLATAVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQ
                     EIAFDMSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGAT
                     ASRSA"
BASE COUNT         1081 a         1296 c         1109 g         1117 t
ORIGIN      
        1 tttcagaaaa agcgctagtc cgggcataaa cacccaaaac aaagcccggt tcgcccgggc
       61 tctgcaccga taatacactt aactgtatgc atgcagcgta cgttggcaaa gtgccgaacg
      121 tacgcaatcc tcttttccga accggacgat cccaaccatt tcatcttctt cgaaacgttc
      181 cagcgcgtca cttaatccgg agcacacgcc gcgaggcaaa tcgcattgcg tgatatcacc
      241 gttgacgata accgtcacgt tctccccgag gcgggttaaa aacattttca tttgcgcggc
      301 agtcacattc tgcgcctcgt caagaatgac aactgcattt tcaaaggtac gtccacgcat
      361 ataggcgaac ggcgcggttt ccaccttccc tatttccggt cgcaggcagt actgcataaa
      421 ggaagcccct aagcgccgga ccagcaagtc gtagaccggg cgaaaatagg gagcaaactt
      481 ttctgcgata tcaccaggta agaagccaag atcttcatcg gcttgcagaa ctggacgggt
      541 gacgataatc ctgtcgacat ccttatgtat cagggcctct gccgcttttg ctgcgctgat
      601 ccaggttttt ccgcacccgg cttcgcccgt ggcgaatatc agctgcttac tctcaatagc
      661 ctgcagatag tgcaattgcg cttcatttcg cgcaaggatg ggcgaggtat cgcgactgtc
      721 gcgggccata ccaatggctt ctacgccgct catctgcaca agcgaggtga ccgattcttc
      781 ttcacgctgc ttatggctgc gcgaatcccg tctcagcaca cgttttgcct cgcgacgagc
      841 tttgatcact gctttttgtc ttcccatgga gagcaccttg agttgtttgt attcatcaca
      901 cgcgccgttg gcagcgcgat tatgcgcacg aacatcagag ggttggcttc cttgtaagcc
      961 atagtttgct tttggataaa atgccgaaaa acggctacgc acaccgtttg cggcgtcggt
     1021 aacacgagta aagaaaggaa gtgagaaaaa tacagagtga cgaagttgct accggaggag
     1081 aaatctccgc gagtggtgga gcgttgctta acaaaacatt gtccagaaca ggacgttacc
     1141 atccgcgatc tccatagtga ctgactatca ctgccgggaa cttccgctgc tacttaataa
     1201 gtacaacaga actcgcattc attgcaacaa tatattcact tatatttaac gataacaccc
     1261 atttcggtga cattaatttc tactggaaag atgacagagt gatgacagtg atggaaaaag
     1321 ctgtgtgctt tccgcaggat tcgcagggct ggtaaaatgt gcctcgtgtt tgccggattc
     1381 ggcgcgaacg ccttacccga cctacaagtg attaataatt tgtgcacaaa actcatgccc
     1441 gataaacctg cgcatcgggc atatttgcgt cgttcaaacc tgcaatagcg cccttcccag
     1501 ataatcgttc gcgtccttca ctcccggcag cgaaaaaaaa taaccgccgc cgataggttt
     1561 aacgtattcc tccagcgctt cgccattgag ccttttttgt actgtcagga agcctttttc
     1621 cagatcgtgt tggtagcaga caaacagcaa ccccatatcc agttgtccgg agttggtgac
     1681 ccccaacgaa taactgtagc cgcgacgcag catcaggctg gactcactct ccgccgtgcg
     1741 gggattcgcc agccggatat ggctgtccag cgcgatcacc ttcccttccg ggtcgctggc
     1801 gtaatcaggc acatcatgct catgctgcat tcccagcggc gcaccggttt gcttatcacg
     1861 gccaaaaatc gtctgctgtt ctttcagcgg cgttctgtcc caaaattcca ctcgaaactg
     1921 aatcaagcgt actgcctgat agctgccacc gattgtccac gcaggctcct gctgatctgc
     1981 cgttacccac accacttttt gcatcaactt atcattctgg ctatcgggat tggcagtgcc
     2041 gtctttgaaa cccagcaaat taatcggcgt ctctttgcct ttactacgcg ccgcgtgatc
     2101 ggaaataaac ccttcccgct tccagcgcac actgagcaaa tccggcgtgt gtttgatgat
     2161 atcgcgcagc gcatggttca ccgtgtcctg ggtgttggcg caaatctgta gcaacacatc
     2221 accatgacat aacgccgcat ccagcgagtc gttggggaaa cgcgtcatct tctgcagctt
     2281 ttttggcatc tgtggcgcaa ggccaaagcg ctcatcaaac aatgagtgac ccaccgataa
     2341 cgtgatggtg agattatcgg gcgcaatgta gccgccaaga atgccggaat cgagtggtgg
     2401 caggcgcgga tttggcgttt ctggtgctgc tccgccctga ctcagaaaag caaaacgctg
     2461 agtcaacaag cgaaacaacc gctcaagatc ggctttatcg ctggcaagca catcaaacgc
     2521 caccagcatc attgcggcct gttgtggcgt caggatccct gcctgatgct caccataaaa
     2581 cggctgtttc tcattgcgtg catccggtga aagcgtgccc ggtgcacttt gcgttttttg
     2641 tgcatgagcg accggacaac ttcccgccag cgcaccaatc cctttcagta aacgtcggcg
     2701 tgacggttca ttcacgccgt tttcatcttt atactgcata acgcttaatc cagtcccagc
     2761 acaccgcgaa gttgcgccag atcttccgcc agcgcagtaa tcggcccttt cagcgcattc
     2821 cggtcggcat cggtcaattt gtcgtaggtt tcaaaaccgt ctttagtacg gtatttcgcc
     2881 agaatggtat cgaccttttt aaagttggca tcgacttttg ccaacagttc cgggttggct
     2941 ttttgcagtt gtggacgcag cagatcgaca attttctgcg agccttcaac gttagcctgg
     3001 aaatcccaca gatcggtgtg gctgtagcga tcttcttcac cgctgatttt gctggctgcc
     3061 acttcctcaa tcagtccggc tgcgccgccg accacttttg aaggtgggaa agccagttca
     3121 ctgatgcgtt tttgcaaatc gaccacatcg gtataaagct gctcagcgta ctgatccatc
     3181 cctttggtgg tgttgtcgcc aaacaatgct ttttccagac ggtggaaacc agtgaatttt
     3241 gggtcggcgg ctttttgctc gtaatcatct tcacgggcgt caatgctgcc atccagatcg
     3301 gagaacagtt cagcaatcgg ttcaatacgc tcatagtgct ggcgcgtcgg cgcatacagt
     3361 gctttcgctt tttcgatatc gcctgcttta atcgcgtcgg taaaggcttt ggtgtcggtc
     3421 accagctgcg tggtttccgc catgacatac gctttatatg cagtaattgc accaccaaga
     3481 cttaacagcg catcactttg cgccgcatcc gccgttgcct cacctttgac gatcaacttc
     3541 cctttcgggt tagtcagcag accgcaggtc atatcgtatt cgccaggctg taaattcgcc
     3601 gtcattttct ggctaaaacc aggggcgata ttttcccgct cttccaccac catcacgcct
     3661 ttgaggatct cccactccag cgccttctgg ctatggttct gaataatgaa ctgtgttttc
     3721 ccggcgttaa ccgtaatggt catcggttcg cactgcttat ccgtcacggt cactttgacc
     3781 tgcggcacat cagcggcgtt agccataaaa gcagaagaaa acagcgcagc cacgctcaac
     3841 tgcaatgcgt tacggcggaa gttaatggtc atgacgagac tatcccttta aagtatgttg
     3901 taactaagca agagtttgcg tcgtatttgt tacgcggagc gagacgctgt cgcccctgcg
     3961 cgtggtggca gagcaaatgc caccagcgcc gggatgagat aaataaacca gacggcgact
     4021 tcgctgacgc tcggtgcttc ctgatagcca aaaatccctt ccatcagcgt gccaaacagc
     4081 gagtgagttg agaggaccgc gctcatatcg aaggcgattt cctgaaagtg gttccacaat
     4141 ccggcttcat gaaatgcgcg aatggcacca gccgccagcc ctgcggcgac gaagagaata
     4201 aacaggctgg tccatttaaa aaatgcacca agattgaggc gaataccgcc ccagtagagc
     4261 aggaagccga gcaccacggc agtagcaaga ccgagcattg cacccagcgg cggccagatc
     4321 ccgacatctt gttgaaatgc cgccagcagg aaaaagaccg actccagccc ttcccttgca
     4381 acggcaaaaa agaccatcat caccagcgcc cagccatgat gatttccacg ctgcaatgcg
     4441 ctatcgactg cctgttccag ttgcactttg acgttgcgcg acactttgcg catccagaaa
     4501 accatccagg taaggatcac cacggcgatc accgccacga taccttcaaa cagttcctgc
     4561 tctttttgcg gaaattcgcc ggtggtttcg ttaatgaaga tcc
//
LOCUS       DAZPCS010000220         4494 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZPCS010000220 DAZPCS010000000
VERSION     DAZPCS010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4494
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.223"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..595
                     /gene="ospD1"
                     /locus_tag="ACHUQ7_003762"
     CDS             <1..595
                     /gene="ospD1"
                     /locus_tag="ACHUQ7_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 3 secretion system effector/anti-activator
                     OspD1"
                     /protein_id="HFR1136965.1"
                     /translation="KGMKNNIINVTNTAISHAINEEKSGGGYSGVSFRKLAKIQNISI
                     PTKNNKEYNRHNLFSLIWHGNADAARKYGESLLAAEIPKEEKLEVLAARNNAGESALF
                     IALQEGHSAAIQAYGDFIKTFDLSPKETIKLLDVRDNEGLPGLFLAAGKGNIEAMMAY
                     INICHHSGIKLTEIADRLNNNEQDMFNIISDKIQELF"
     gene            919..1029
                     /locus_tag="ACHUQ7_003763"
                     /pseudo
     CDS             919..1029
                     /locus_tag="ACHUQ7_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1107..1421
                     /locus_tag="ACHUQ7_003764"
     CDS             1107..1421
                     /locus_tag="ACHUQ7_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1136966.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            1703..2269
                     /gene="ipgB2"
                     /locus_tag="ACHUQ7_003765"
     CDS             1703..2269
                     /gene="ipgB2"
                     /locus_tag="ACHUQ7_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB2"
                     /protein_id="HFR1136967.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYRISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(2531..2653)
                     /locus_tag="ACHUQ7_003766"
                     /pseudo
     CDS             complement(2531..2653)
                     /locus_tag="ACHUQ7_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            2796..3350
                     /locus_tag="ACHUQ7_003767"
                     /pseudo
     CDS             2796..3350
                     /locus_tag="ACHUQ7_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
     gene            3689..4050
                     /locus_tag="ACHUQ7_003768"
                     /pseudo
     CDS             3689..4050
                     /locus_tag="ACHUQ7_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            4137..>4494
                     /locus_tag="ACHUQ7_003769"
     CDS             4137..>4494
                     /locus_tag="ACHUQ7_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1136968.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRAFIR
                     SLSVPQEQEPAVRFETE"
BASE COUNT         1345 a          951 c         1010 g         1188 t
ORIGIN      
        1 taaaggaatg aaaaataata tcattaacgt gacaaatacc gctatatccc acgccatcaa
       61 tgaagaaaaa tcaggggggg gatatagtgg tgtttctttc agaaaattgg ccaaaataca
      121 gaacatatcc attccgacaa agaataataa ggagtataac cgccataatt tgttttcatt
      181 gatttggcat ggaaatgccg atgcagcgcg taaatacggt gaatcgctgt tggcagccga
      241 aatacccaaa gaggaaaaac tagaagttct tgcagcacga aataatgctg gggaatctgc
      301 tttgttcata gctcttcaag aaggtcattc cgctgcgatt caagcttatg gagattttat
      361 taaaactttt gatttatcac caaaagaaac gattaaacta ttggatgtaa gagataatga
      421 ggggttacca ggattatttc tggccgcagg gaaagggaat atcgaggcta tgatggcata
      481 tataaatata tgccatcata gtgggataaa acttacagaa atagcagaca gacttaacaa
      541 taatgaacaa gacatgttta atattatttc tgacaaaata caagagttgt tttaagtgtg
      601 ctaaatagct gcaaagaatt gcacttagaa ggatctctcc ccgaattgtg tgatctgctt
      661 aatttctaaa aagaaacaaa cagttaacta gactgagcga tactacccat agaaccagtt
      721 tcccatgacg aacgacactg gacgcaaaaa caatcataaa aaacacaata aatgtaatga
      781 acacatccca ccctattaag taaagtgcac tgtgatagag gaagggaaat acaggaaggt
      841 gtggagcctt gcagatttct ggcatcatag tgcccaatac ggaatgcata aagccctctc
      901 agactggaga ctccacaaat gaaatataca gtgtttaatt atatcaagtg cgattacaat
      961 cgctgacgac ggcacagttg gtgtggaggt ctcagtcctg aacaatttga aaaccagaac
     1021 ctcacttaag cctgtgttca tattacgtgg gtaggatcat acttttatag aagctgtcag
     1081 cggctacgtc ccagccaaat cacttcatgc tccagcgcca gagaggcaag gttgggtttg
     1141 ctcagctgcc ggttgatttt gttgcgattg aaccggacag cgtccagggc gtcggcaaaa
     1201 gagcgaatct caccaatcgt tgtttcatta tccgcattaa cgatagcttc aagaaacgcc
     1261 aaggttttat cgaatttatc agcaacagtg gctccgggca taccgtgacc gtttatacaa
     1321 aacggcgttt tcagcgtgga gttttcatga atagcctcaa cacgaatgtc gtgaagccag
     1381 tgatgtatcg gctacgatcc tcagctgatg ccagaccata ggactgacac tacgaacagc
     1441 aattttaatg acgtaaatct tcataactac aactaacagg gcatcattat gaatgatgcc
     1501 ggttatttta ccgctgttgg tcccttcatt tagggactgc cccaagtttt ttcatactct
     1561 ctttctcaga gagtttagtc tctgggattc cagacggtcc atcattgagc caagggagta
     1621 aagaactcaa agcatttaca gaattgtttg aatcagctaa aataatgaca atgttactat
     1681 tttacatgac aaggtgatta taatgcttgg aacatctttt aataattttg gaatcagttt
     1741 atctcacaaa agatattttt caggaaaggt ggatgagatt atccgctgta caatggggaa
     1801 aaggatagta aaaatatcat caaccaaaat aaatactagt attttgagct ctgtttcaga
     1861 acaaatcggt gaaaatatta cagattggaa aaatgatgaa aaaaaagtct acgtatcccg
     1921 ggtggtaaac caatgtattg ataagttttg tgcagaacac agccgaaaaa tcggcgataa
     1981 tctccgtaaa cagatattta aacaggttga gaaagattat cgcatttctc tagacatcaa
     2041 tgctgcacaa agttccataa atcatcttgt atcaggaagc agttatttca aaaagaaaat
     2101 ggacgaactt tgtgaaggga tgaatagaag cgttaaaaat gatacaacaa gcaatgttgc
     2161 aaatcttatt tccgatcagt tttttgagaa aaatgtgcaa tatattgatc taaagaaatt
     2221 acgtgggaat atgagtgact atattacaaa tttagaatcg cctttctgat gacagaacat
     2281 cccgagtaaa gaaactaaga aaagtaaata cccaggaatt gcctgagcgg tgctatattt
     2341 ctcctcgcaa gtaagtccta atatattgaa ttgttgtata gccagttttt gtattttcct
     2401 agctcatgga tgtgggatcc ctctcccctt tctctgctca aaggtaagcg gctcgtcaga
     2461 atcatattga tatttactga gaagtcagat caactttcca aggcaacaga tcgcgtaccc
     2521 ggtttgccgg tcagtcctgg atatgttcaa taacgtaacg cagccatttt tctggctcca
     2581 cattgttcag acggcatgtg ccgatcagcg agaacaacac cgctgcatgt tcgtcaccgc
     2641 tgtcggaacc tgcgagtcga gtcagccgga gagagtttca ctctccggct gcttacggaa
     2701 ccgtacgtga cactctcgcg tcatacggct cttgatcact cattcgatgc acgtagtaaa
     2761 taccagtgag caaattgatc ttacccagca atacggtcag ttaccgcgca catggcctgc
     2821 ctgtgtcaga aaatctgctg gagcaggatt tttacgccag tggtccgaac cagaagtggg
     2881 cgggagacat cacgtactta cgtacagatg aaggctggct gtatctggct gtggtcattg
     2941 acctgtggtc acgtgccgtt attggctggt ccatgtcacc acgcctgacg gcgcaactgg
     3001 cctgcgatgc cctgcagatg gcgctgtggc ggcgtaagag accccggaac gttatcgttc
     3061 actcggaccg aggaagtcag tactgttcag cagattatca ggcgctgctg aagtggcata
     3121 atctgcgtgg cagtatgagc gcaaagggct gctgctacga taatgcctgc gtggaaagct
     3181 tctttcattc gctgaaagtg gaatgtctcc acggagaaca ctttatcagc cgggaaataa
     3241 tgagggcaac agtgtttaat tatatcgaat gtgattacaa tcgctggcga cggcacagtt
     3301 ggtgtggcgg cctcagtccg gaacaatttg aaaaccagaa cctcgcttag gcctgtgtcc
     3361 atattacgtg ggtaggatca cttacgccct gtggttgaaa cgtgagcata ttgttttcag
     3421 atgctgttgt cttgcttcag agtgatctgt cctccggatt tacgcaaagt tatccacagg
     3481 cactgtgcat ttaaaaaact tatcttttct tcttgatttt aaaaactcag atcgtcatat
     3541 cattatgata aacataataa aaaactctaa acatgagaag gtttttaacg taaggtgaga
     3601 ggatttttaa gtgtatccat agattaaaaa tgacaatgcc tccaattagt tgaattaggg
     3661 gttataatac ccgcttttag gttctttcat ggtcacagtt aactttcatt gcccccgttg
     3721 ccagtccgtt caggtttacc gtcacgggca gaacccaaaa ggacatgatc gattccgttg
     3781 ccgtgactgt caccgggtgt ttcagctcac ttactgttac gaggcccgta aaccgggagt
     3841 taaaaaccaa atcactgaaa tggctttcaa cggtgccgga gttcgcgata ctggccgtac
     3901 gctgaaaatc ggcatcaata ccgtcatacg gacattaaaa agctcccgcc ccggcggata
     3961 acatctgcgc cagtcgctca cgctgatgtt gcccttatct gtgaacttga tgggcagtgg
     4021 agcttcgttg gcagcaaggc gcaacaacac tgtcacgaac ggtgcaatag tgatccacac
     4081 ccaacgcctg aaatcagatc cagggggtaa tctgctctcc tgattcagga gagcttatgg
     4141 tcacttttga gacagttatg gaaattaaaa tcctgcacaa gcagggaatg agtagccggg
     4201 cgattgccag agaactgggg ctctcccgca ataccgttaa acgttatttg caggcaaaat
     4261 ctgagccgcc aaaatatacg ccgcgacctg ctgttgcttc actcctggat gaataccggg
     4321 attatattcg tcaacgcatc gccgatgctc atccttacaa aatcccggca acggtaatcg
     4381 ctcgcgagat cagagaccag ggatatcgtg gcggaatgac cattctcagg gcattcattc
     4441 gttctctctc ggttcctcag gagcaggagc ctgccgttcg gttcgaaact gaac
//
LOCUS       DAZPCS010000221         4405 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZPCS010000221 DAZPCS010000000
VERSION     DAZPCS010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4405
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.224"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            120..977
                     /gene="focA"
                     /locus_tag="ACHUQ7_003770"
     CDS             120..977
                     /gene="focA"
                     /locus_tag="ACHUQ7_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFR1136969.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWCQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            1032..3314
                     /gene="pflB"
                     /locus_tag="ACHUQ7_003771"
     CDS             1032..3314
                     /gene="pflB"
                     /locus_tag="ACHUQ7_003771"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFR1136970.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            3506..4246
                     /gene="pflA"
                     /locus_tag="ACHUQ7_003772"
     CDS             3506..4246
                     /gene="pflA"
                     /locus_tag="ACHUQ7_003772"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFR1136971.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(4328..>4405)
                     /locus_tag="ACHUQ7_003773"
     CDS             complement(4328..>4405)
                     /locus_tag="ACHUQ7_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1136972.1"
                     /translation="FDGEELHASYYQSLLSQVRDMVDAL"
BASE COUNT         1117 a         1097 c         1076 g         1115 t
ORIGIN      
        1 tgcataaaaa ccatgcgagt tacgggccta taagccaggc gagatatgat ctatatcaat
       61 ttctcatcta taatgctttg ttagtatctc gtcgccgact taataaagag agagttagtg
      121 tgaaagctga caaccctttt gatcttttac ttcctgctgc aatggccaaa gtggccgaag
      181 aggcgggtgt ctataaagca acgaaacatc cgcttaagac tttctatctg gcgattaccg
      241 ccggtgtttt catctcaatc gcattcgtct tctatatcac agcaaccact ggcacaggca
      301 caatgccctt cggcatggca aaactggttg gcggcatttg cttctctctg gggctgattc
      361 tttgtgttgt ctgcggagcc gatctcttta cttccacagt gttgattgtt gttgctaagg
      421 cgagtgggcg catcacctgg tgtcagttgg ctaaaaactg gctaaatgtc tattttggca
      481 acctggtcgg cgcactgctg tttgtacttt taatgtggct ttccggcgag tatatgaccg
      541 caaatggtca atggggacta aacgtcctac aaaccgccga ccacaaagtg caccatactt
      601 ttattgaggc cgtctgtctt ggtatcctgg caaacctgat ggtatgtctg gcagtatgga
      661 tgagttattc tggccgcagc ctgatggaca aagcgttcat tatggtgctg ccggtcgcga
      721 tgtttgttgc cagcggtttt gagcacagta tcgcaaacat gtttatgatc ccgatgggta
      781 ttgtaatccg cgacttcgca tctccggaat tctggaccgc tgtcggttct gcaccggaaa
      841 atttttctca cctgaccgtg atgaacttca tcactgataa cctgatcccg gttacgatcg
      901 gtaatattat cggcggtggt ttgttggttg ggttgacata ctgggtcatt tacctgcgtg
      961 aaaacgatca ccattaatgg ttgtcgaagt acgcagtaaa taaaaaatcc acttaagaag
     1021 gtaggtgtta catgtccgag cttaatgaaa agttagccac agcctgggaa ggttttacca
     1081 aaggtgactg gcagaatgaa gtaaacgtcc gtgacttcat tcagaaaaac tacactccgt
     1141 acgagggtga cgagtccttc ctggctggcg ctactgaagc gaccaccacc ctgtgggaca
     1201 aagtaatgga aggtgttaaa ctggaaaacc gcactcacgc gccagttgat tttgacaccg
     1261 ctgttgcttc caccatcacc tctcacgacg ctggctacat caataaagcg ttggaaaaag
     1321 ttgttggtct gcagactgaa gctccgctga aacgtgctct tatcccgttc ggtggtatca
     1381 aaatgatcga aggttcctgc aaagcgtaca accgcgaact ggacccgatg atcaaaaaaa
     1441 tcttcactga ataccgtaaa actcacaacc agggcgtgtt cgacgtttac actccggaca
     1501 tcctgcgttg ccgtaaatcc ggtgttctga ccggtctgcc agatgcttat ggccgtggcc
     1561 gtatcatcgg tgactaccgt cgcgttgcgc tgtacggtat cgactacctg atgaaagaca
     1621 aatacgctca gttcacctct ctacaggctg atctggaaaa cggcgtaaac ctggaacaga
     1681 ctatccgtct gcgcgaagaa atcgctgaac agcaccgcgc tctgggtcag atgaaagaaa
     1741 tggctgcgaa atacggctac gacatctctg gtccggctac caacgctcag gaagctatcc
     1801 agtggactta cttcggctac ctggctgctg ttaagtctca gaacggtgct gcaatgtcct
     1861 tcggtcgtac ctccaccttc ctggatgtgt acatcgaacg tgacctgaaa gctggcaaga
     1921 tcaccgaaca agaagcgcag gaaatggttg accacctggt catgaaactg cgtatggttc
     1981 gcttcctgcg tactccggaa tacgatgaac tgttctctgg cgacccaatc tgggcaaccg
     2041 aatctatcgg tggtatgggc ctcgatggtc gtaccctggt taccaaaaac agcttccgtt
     2101 tcctgaacac cctgtacact atggggccgt ctccggaacc gaacatgacc attctgtggt
     2161 ctgaaaaact gccgctgaac ttcaagaaat tcgccgctaa agtgtccatc gacacctctt
     2221 ctctgcagta tgagaacgat gacctgatgc gtccggactt caacaacgat gactacgcta
     2281 tcgcttgctg cgtaagcccg atgatcgttg gtaaacaaat gcagttcttc ggtgcgcgtg
     2341 caaacctggc gaaaaccatg ctgtacgcaa tcaacggcgg cgttgacgaa aaactgaaaa
     2401 tgcaggttgg tccgaagtct gaaccgatca aaggcgatct cctgagctac gatgaagtga
     2461 tggagcgcat ggatcacttc atggactggc tggctaaaca gtacatcact gcactgaaca
     2521 tcatccacta catgcacgac aagtacagct acgaagcctc tctgatggcg ctgcacgacc
     2581 gtgacgttat ccgcaccatg gcgtgtggta tcgctggtct gtccgttgct gctgactccc
     2641 tgtctgcaat caaatatgcg aaagttaaac cgattcgtga cgaagacggt ctggctatcg
     2701 acttcgaaat cgaaggcgaa tacccgcagt ttggtaacaa tgatccgcgt gtagatgacc
     2761 tggctgttga cctggtagaa cgtttcatga agaaaattca gaaactgcac acctaccgtg
     2821 acgctatccc gactcagtct gttctgacca tcacttctaa cgttgtgtat ggtaagaaaa
     2881 ctggtaacac cccagacggt cgtcgtgctg gcgcgccgtt cggaccgggt gctaacccga
     2941 tgcacggtcg tgaccagaaa ggtgctgtag cgtctctgac ttccgttgct aaactgccgt
     3001 ttgcttacgc taaagatggt atctcctaca ccttctctat cgttccgaac gcactgggta
     3061 aagacgacga agttcgtaag accaacctgg ctggtctgat ggatggttac ttccaccacg
     3121 aagcatccat cgaaggtggt cagcacctga acgttaacgt gatgaaccgt gaaatgctgc
     3181 tcgacgcgat ggaaaacccg gaaaaatatc cgcagctgac catccgtgta tctggctacg
     3241 cagtacgttt caactcgctg actaaagaac agcagcagga cgttattact cgtaccttca
     3301 ctcaatctat gtaattagat ttgactgaaa tcgtacagta aaaagcgtac aataaaggct
     3361 ccacgaaagt ggggcctttt ttagcacgag agcctttttt gtcagctatc tatactttaa
     3421 ggtgactgcc aaaacagact cgacgtagcc ttcgagctgc gcaccaacac ggcctcagat
     3481 gggccacatc tggagaaaca ccgcaatgtc agttattggt cgcattcact cctttgaatc
     3541 ctgtggaacc gtagacggcc caggtattcg ctttatcacc tttttccagg gctgcctgat
     3601 gcgctgcctg tattgtcata accgcgacac ctgggacacg catggcggta aagaagttac
     3661 cgttgaagat ttgatgaagg aagtggtgac ctatcgccac tttatgaacg cttccggcgg
     3721 cggcgttacc gcatccggcg gtgaagcaat cctgcaagct gagtttgttc gtgactggtt
     3781 ccgcgcctgt aaaaaagaag gcattcatac ctgtctggat accaacggtt ttgttcgtcg
     3841 ttacgatccg gtgattgatg aactgctgga agtaaccgac ctggtaatgc tcgatctcaa
     3901 acagatgaac gacgagatcc accaaaatct ggttggagtt tccaaccacc gcacgctgga
     3961 gttcgctaaa tatctggcga acaaaaatgt gaaggtgtgg atccgctacg ttgttgtccc
     4021 aggctggtct gacgatgacg attcggcaca tcgcctcggt gaatttaccc gtgatatggg
     4081 taacgttgag aaaatcgaac ttctccccta ccacgaactg ggcaaacaca aatgggtggc
     4141 gatgggtgaa gaatacaaac tcgatggtgt taaaccaccg aagaaagaga ccatggaacg
     4201 cgtgaaaggc attcttgagc agtacggtca taaggtaatg ttctaatttc ctcgtaaagc
     4261 ggcaacttta ttgagttgcc gcttttttat tccgcgcttc attctgctac agcgttttta
     4321 acacatctta tagtgcatct accatatccc gtacctgaga taataacgac tgataatagc
     4381 tcgcgtgaag ttcttcccca tcgaa
//
LOCUS       DAZPCS010000222         4385 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZPCS010000222 DAZPCS010000000
VERSION     DAZPCS010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4385
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.225"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..195)
                     /locus_tag="ACHUQ7_003774"
     CDS             complement(<1..195)
                     /locus_tag="ACHUQ7_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1136973.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNDLRYMI
                     YPHAHPKDQVNLWLQIHTGSL"
     gene            complement(252..2612)
                     /locus_tag="ACHUQ7_003775"
     CDS             complement(252..2612)
                     /locus_tag="ACHUQ7_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFR1136974.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMYFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRLDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIDKNVIARAS
                     EAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQIVFS
                     YIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFTHQPS
                     GLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSWTPQF
                     LKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            complement(2650..4335)
                     /locus_tag="ACHUQ7_003776"
                     /pseudo
     CDS             complement(2650..4335)
                     /locus_tag="ACHUQ7_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
BASE COUNT         1194 a          977 c          971 g         1243 t
ORIGIN      
        1 caatgaaccg gtatgaattt gcagccataa atttacctga tcctttggat gagcatgcgg
       61 ataaatcata tatcgcaagt cattgtccag ttgcccggta attaactttt catcctgcgg
      121 taaggcggcg gcaatcagcc gcccggggag caacagagtt gccactaacg tcagtaagaa
      181 acagaggttt ctcataatta tctccatgcg aaaaccgggc gaatttaccc ggttaagtaa
      241 aatccgaact attaaaattt catgctgaca tcaagccaga aagtacggcc actggcgtag
      301 gtcgccacac cggtgttcgt tgtatcaaca gcggttttgc tatccagtac attgagaata
      361 tcggcactga tcgtcaggtt ttgttgtttc agaaattgtg gcgtccagga caagcgggtg
      421 tcccaggtca ggctgctatc cagtttttcg tcaacatact gcttgtaatc tgaatattcg
      481 ctgatgtatt gcgcatttgt cttaccaagg ataatgcgag ctttacgcgc ttcttgccag
      541 gccagcgtat tcgcccacac taacccgctc ggttgatgcg tgaaatccat gtttaaggag
      601 atctttaatg ggttattaaa atctgccact ggaacgctat cgtaagagac cagattaccg
      661 ttataaacca cctggttatc accggtattg ctctcctcat aaccattgtt taacgacaag
      721 ttgcccttgc tcttgatata gctaaagaca atttgcgggt taatatctac ctggcggata
      781 tgcaggggtt cggccagttc aaaactgagg ttaaacgaat gcgttttggt tttgccatcg
      841 ttgttatatt cagtaatggt ggttttagtc gcgctgtcgg tacgactgct tttgctgatt
      901 tgatcatgcg cttcacttgc gcgtgcaata acgttcttat cgattttttg ctgcaatccc
      961 attgccagtt catcgttata aggcgttttc aaattctgat aacgcgtcag ggttttatta
     1021 cctgataccg attccgtcca gctattgcgg atatcacgta atcccatatc aagaatattc
     1081 ccgccatagt aacggttata accggcggta atcattgagg tttgatcagc aaaaatatcc
     1141 cattccgtca taaagcgcgg ggagatattg tggtttgaca gatagttgtc atagtcataa
     1201 cgcacaccgg gcattaacga cacattccgc cagctaatgt gatccgccat atacagcgtg
     1261 tagttgtcaa ttcccaggct gcctttacct ttatggtaaa tggtatggtt agttttcttt
     1321 cctgcagcat taatcacata ggattcagac tggttatggc gttcagtcca tgcatcggaa
     1381 tagatgtatt ccgcgccgaa gtagggttga tgcgaaacat cacccacggc gaatttttgc
     1441 cagtccaggc gtgttttgaa ggtgtaatta tctacagcct gggaaatgtg tcctaatccg
     1501 ccacgggtac aacgccctgt aatatcacca tatgtacatg aaagttcggt gtaccagatg
     1561 tcatgatcgt gacgggtata atcactaata tgatcccaac caacggtggt acgtagtttg
     1621 gcccatgcga gttgcgtatc catatcccat gccagaccat aggatttatt acccatttcg
     1681 cgatcagact gcgggaaggt gctggtatta taatcacggc tggagccggt atattttaag
     1741 gttaaatcgt gggtaaagcg gtcgctggca aaccaggtaa atttgctcaa tgcagtatcg
     1801 ataacgtttt tatactgtgc ccgaccggcg acaatgccgt cattcgaaac ataatccgcg
     1861 cgggtgatat cagactggcg gcgcgataaa ccggcggtaa cgccaaagtt atcagccagc
     1921 tcctgattaa acgacaaggt ataaaagttc tttttaaaat ctggagagaa ataggtactt
     1981 cctgaagaac cttgattaaa tgcgctcttg ttattctcat cgatatgcga tgttaaccag
     2041 tccaaacgcg tagtgcgata acccaatttc accttgctat catcagcgtt gaagcgtttg
     2101 atctttgcat caattacccc gccattgaag cgaccaaatt caaccggcac aaaactgtca
     2161 taaagcgtca cattgtccag taagctgaca tcaagataat acccctgtga catcccgcta
     2221 atattggttg cactactggc atcggactca ttcgctgggt tcaggttatt agttgcacta
     2281 ataccgtcaa tcaaataggc attctggtag ggcgacgcac cgtgaataga gattttctca
     2341 gggcgaatat ctccctggtt caacgaggta ctttgcgttg aatccatgcg taccgcaggg
     2401 ttggttctca gcagatcgct gatattgcca ttcccggtcg gtaaacgctc gatgctttca
     2461 ctggtgtaat gcgtgttgcc gtttacgggt acagggacag gagcatagac cgtcatctct
     2521 tcaaaaaaat acatgtacat gtttttgtca tcgacggcct gcgccacatc agcgccacat
     2581 aaaatgacgc caggaataag aactcgcttc atttatttat atctccgtac agcagggctt
     2641 ttattgttgt tataaaaccg tgctaatgtc acaaatatca tcagccaggt tccagacacc
     2701 gggttgatgt gtaaccataa tgacgccgct tgtgggtagt ttttcacgca ctaaacgcag
     2761 taagcgtata gcctcttgtt cctcaagatg agaggtagtt tcgtcaagaa atatccattt
     2821 cggacgtcgt aaaattaatc gtgccagggc gatacgttgt ttttcgccgc tggaaagaat
     2881 atctccccag cgatcgtggt catgaatacg cgcagccaat tttcccagac caacctgatg
     2941 cagtacttcg ctcaacgatt tatcgtctac gggcagggga agtgctttac aaataatctc
     3001 tttcagtaag ccgcttttga ttaacggtgt ttgtgacaca taccaactgt cagcaggaga
     3061 agaaatatca cctttaaacc acggccagca gtgggataat gttttaagca gtgtggtttt
     3121 tcccgcacca gagtagcctt tcagtaatag ccatttgcct ggcgaaacat gaaagttcaa
     3181 gttctctaat atgatcttat tatcaggcgt acgaatactc gcattagcca cttgtaccgc
     3241 atgttggcaa tttttaggct tattcgtagg gcgctattca gtgagttgat ggaactcata
     3301 caagcgatcg ataaccgcag ccagttcagc aagttcgtca tatttataaa taaaccagct
     3361 taaattgttc gatactagca taaatgcctg gcgcgatttc atcagtccgc ccagattgat
     3421 ctgcccgcta ataaactgcg gcaataacag aaagtaggga agaacgctaa gcgagcgcga
     3481 ataaatattt tgccagtaat caagccaccg ctgacgattc attaaacgat gccagttctc
     3541 tttaatggta tgaaaattgt cgcgcaactc ctggcgttgt agagactcag cattgcttag
     3601 cgcaatcagt tctgcctgct tattatgctg cacaagatta gttcgaaatg tcgcttcgct
     3661 acgttgtttt tccacattaa gcggacgaat acgtttaccc accttatggg taaataaagt
     3721 tccaccgatc acaatgagca cgacggtata gaccatatac ccctggatat tccattctgt
     3781 tccaccaaca gtgaatgaga gcgtacccgc gctttgccaa agaataacgg taaaggtgat
     3841 cagcatgcta agtgactgga tgaagccaaa tgaaagactg agcgttttgt tgatcagtaa
     3901 gagaatgtct tcagcgatac gttggtcagg gttatctgta tttttatgct cgccgtagat
     3961 ctgcgtgaag tagtagtttt tatctgcaaa ccagcgatta aggtagtaat ctgttaacca
     4021 ttcgcgccag cgaatagtca gtagtttaat taaccatgtt ttgtttgcgg atattaatac
     4081 aaagatcccc agcagggcag ggaaccaaag aacgagctgc caaagcttat cggtttcttt
     4141 ctggcttagc gcattgaaaa aatcattatt ccagtcgttt aaccagacct gaattttaac
     4201 cacgccaagg atcattgcga ggataataat gattaacaac actgaagttt tattattctt
     4261 acgcagccaa aagggcttca gcagacagag atactttgct atcaacatac aaaacgtaat
     4321 gggaatggtt atcattagcg aaaattgatt gtgccatcca atgattatct atggcaatga
     4381 tttac
//
LOCUS       DAZPCS010000223         4320 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZPCS010000223 DAZPCS010000000
VERSION     DAZPCS010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4320
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.226"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(204..950)
                     /gene="cutC"
                     /locus_tag="ACHUQ7_003777"
     CDS             complement(204..950)
                     /gene="cutC"
                     /locus_tag="ACHUQ7_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFR1136975.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(964..1530)
                     /locus_tag="ACHUQ7_003778"
     CDS             complement(964..1530)
                     /locus_tag="ACHUQ7_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR1136976.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSEKMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1746..3479
                     /gene="argS"
                     /locus_tag="ACHUQ7_003779"
     CDS             1746..3479
                     /gene="argS"
                     /locus_tag="ACHUQ7_003779"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFR1136977.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDTAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDVFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(3521..3565)
                     /locus_tag="ACHUQ7_003780"
     CDS             complement(3521..3565)
                     /locus_tag="ACHUQ7_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1136978.1"
                     /translation="MSVFRIHLDGNKKA"
     gene            3656..4144
                     /locus_tag="ACHUQ7_003781"
     CDS             3656..4144
                     /locus_tag="ACHUQ7_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFR1136979.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDTSAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKLDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
BASE COUNT         1146 a         1067 c         1045 g         1062 t
ORIGIN      
        1 gttttagcaa tttaaatccc ataatcaatg tgaatataaa aaatattttt agcacaacgc
       61 tatgcgcact gtcacccgct caacagatca cggttatcgt tcgtttttta tactgctcag
      121 ggataaaaaa aggcccctgt tgaaattgca ggggcctggt acgagcaagc atcatattgg
      181 gcgacatgat gcaacggtaa aaatcatttg gcctgatggc gttcaatgat tcctttcatt
      241 tcagcaaccg ccgccccgtc tacgatataa cgcgaatact cgtccgcgtg ttcatctgat
      301 gacatggaca atccttgatt acgataacgc atcggtgagg cttgccacgc tcccgcggag
      361 ctatggactt ccagcactcc ggcatcgagg aagtggtgca ggttttctgc acggactcct
      421 gctccagcca taatgattgg agcatcacga tgggcaataa gttccataat ttttgataaa
      481 ccttgcagtg cgtctgattt ttgccctgat gtcagtaccc gggcaatgcc caattccgca
      541 agattattga gtgtatataa agggttagcg cacatatcga aggcgcgatg aaaagtcact
      601 gccagcggac cggcagcagc cattattttt tccattcgtg gcatatcgac attcccgtca
      661 acatcgagaa cgcccgtcac cagtccagga aaacctaatt cgcggaccgt gcgcacatcc
      721 tcaagaatgg cggcaaactc accgtcgctg tagcaaaaat caccaccgcg tgggcgaatt
      781 atcggatgca cagggatcgt cacccgctgg cgcacggatt tcagtacccc caacgacggc
      841 gttaagcccc cctcttttgg ggctgcgcat aattcaactc tgtctgcgcc gttttgctgc
      901 gccgttagtg cacattccat gctgtaacag caaatttcca gtaatgccat ttttgctcct
      961 taattacgct gactgctcgc tggcaacgat ctcttcaatg gaccacggat gaaacttaat
     1021 agttgttttg ccatcggtga ccgccagcgt cggattcggt aatcgctcat gctcaccttt
     1081 tggtgaactg gttttaactg agatccccgg taggctgagt ccttcatcag aaagcaatgc
     1141 cagcgcacgg gcattcagcg tttctggatc acccggcaga acgatttcaa tatgttccca
     1201 accttcgtgt gggtaacgtt tttccccggg ccacggtagc tccacattgg aaaactgcca
     1261 gtgcgcaacc tgtaccggtt catgcaattt aaacagacaa atcggtctgc cattgatcat
     1321 tttttctgac aaaagctcgc cacactgttc aaaaccgcga cgccagcgct cagcggtgac
     1381 gttttgatgg caacgcaaag aaatgtgatc ggcagtcagc ggagtgatat ccaaccccag
     1441 acggcgggaa agttcatcta atgcgtgggt aaatcgcggt aaatccgatg caatatcctg
     1501 cagctcgtcg atagattgcc agttcgccat aatcactctt cgtctttcag taaaagcgtt
     1561 aatttaccct gttgccctgt gccaaccaac cgctgatttc acgccgcttc tgatgcaata
     1621 gtgaaaacgg caatacgcca cgcgcacgtt gctgacgaaa acagccattt gcagtatact
     1681 cccgccctaa tttctttaac tggtgcgggc aatttttgct cgcttcatca atgtaaggta
     1741 ttccggtgaa tattcaggct cttctctcag aaaaagtccg tcaggccatg attgcggcag
     1801 gcgcgcctgc ggattgcgaa ccgcagattc gtcagtcagc aaaagttcag ttcggcgact
     1861 atcaggctaa cggcatgatg gcagttgcta aaaaactggg tatggcaccg cgacaattag
     1921 cagagcaggt gctgactcat ctggatctta acggtatcgc cagcaaagtt gagatcgccg
     1981 gtccaggctt tatcaacatt ttccttgatc cggcattcct ggctgaacat gttcagcagg
     2041 cgctggcgtc cgatcgtctc ggtgttgcta cgccagaaaa acagaccatt gtggttgact
     2101 actctgcgcc aaacgtggcg aaagagatgc acgtcggtca cctgcgctct accattattg
     2161 gtgacacagc agtgcgtact ctggagttcc tcggtcacaa agtgattcgc gcaaaccacg
     2221 tcggcgactg gggcactcag ttcggtatgc tgattgcatg gctggaaaag cagcagcagg
     2281 aaaacgccgg tgaaatggag ctggctgacc ttgaaggttt ctaccgcgat gcgaaaaagc
     2341 attacgacga agatgaagag tttgccgagc gtgcacgtaa ctacgtggta aaactgcaaa
     2401 gcggtgacga atatttccgc gagatgtggc gcaaactggt cgacatcacc atgacgcaga
     2461 accagatcac ttacgatcgt ctcaacgtga cgctgacccg tgatgacgtg atgggcgaaa
     2521 gcctctacaa cccaatgctg ccaggaattg tggcggatct caaagccaaa ggtctggcag
     2581 tagaaagcga aggggcgacc gtcgtattcc ttgatgtgtt taaaaacaag gaaggcgaac
     2641 cgatgggcgt gatcattcag aagaaagatg gcggctatct ctacaccacc actgatatcg
     2701 cctgtgcgaa atatcgttat gaaacactgc atgccgatcg cgtgctgtat tacatcgact
     2761 cccgtcagca tcaacacctg atgcaggcat gggcgatcgt ccgtaaagca ggctatgtac
     2821 cggaatccgt accgctggaa caccacatgt tcggcatgat gctgggtaaa gacggcaaac
     2881 cgttcaaaac ccgcgcgggt ggtaccgtga aactggcaga cctgctggat gaagccctgg
     2941 agcgtgcacg tcgtctggtg gcagaaaaga acccggatat gccagctgac gagctggaaa
     3001 aactggctaa cgcggttggt attggtgcgg tgaaatatgc ggatctctcc aaaaaccgca
     3061 ccacggacta catcttcgac tgggacaaca tgctggcgtt tgagggtaat accgcgccat
     3121 acatgcagta tgcttacacg cgtgtattgt ccgtgttccg taaagcagaa attaacgaag
     3181 agcaactggc tgcagctccg gtaatcatcc gtgaagatcg tgaagcgcaa ctggcagctc
     3241 gcctgctgca gtttgaagaa accctcaccg tggttgcccg tgaaggcacg ccgcatgtaa
     3301 tgtgtgctta cctgtacgat ctggccggtc tgttctctgg cttctacgag cactgcccga
     3361 tcctcagcgc agaaaacgaa gaagtgcgta acagccgtct gaaactggca caactgacag
     3421 cgaagacgct gaagctgggt ctggatacgc tgggtattga gactgtagag cgtatgtaat
     3481 cgatttttcg tgagagtgaa gcctgatcag ggttagccga tcaggctttt ttattgccat
     3541 ctaaatgtat tctgaaaacg gacatgccat tgttttctca ctgttgagta agaggccaga
     3601 agcgtaatat ccaggcccag gaaaacgata acggttcaat ttaaggaata ccgaagtgtt
     3661 taaatttctt gtattaacat taggcattat ctcttgccag gcttacgcag aagatacggt
     3721 tatagtaaac gaccatgaca cttcagccat caaagattgt tggcaaaaaa attcagatga
     3781 tgatactgac attaacgtga tcaaatcatg cctgcgacaa gaatacaatc tcgtcgatgc
     3841 gcaattaaat aaagcctatg gtgaagctta tcgttatata gaacaagtgc cacgcacagg
     3901 tgcaaaaaaa cttgatactg aacaacttaa cttgcttaaa aaatcacagc gagcctggct
     3961 ggattttagg gacaaagaat gtgaattaat cctttcaaat gaggatgttc aggatttaag
     4021 tgacccctat tctgaatcag aatggctatc atgtatgatc atacagacca atacgcgaac
     4081 tcgccagttg cagctatacc gtaactctga agatttttat ccaagccctt tgacaagagg
     4141 ataattcaca tctttttggc atgttttgtt gcaagctatt cctgataaat gattgcaaca
     4201 aaacatccag ccttttgact gagttattaa acatactcgc gagcgcgtaa ttttttgtct
     4261 ttcagcgata attcacaatc acttcattcc gttgtacctt tagcggtgga attaaccgcc
//
LOCUS       DAZPCS010000224         4319 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZPCS010000224 DAZPCS010000000
VERSION     DAZPCS010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4319
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.227"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..118
                     /locus_tag="ACHUQ7_003782"
     CDS             <1..118
                     /locus_tag="ACHUQ7_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1136980.1"
                     /translation="NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(115..381)
                     /locus_tag="ACHUQ7_003783"
     CDS             complement(115..381)
                     /locus_tag="ACHUQ7_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFR1136981.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPSLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            655..4179
                     /gene="nifJ"
                     /locus_tag="ACHUQ7_003784"
     CDS             655..4179
                     /gene="nifJ"
                     /locus_tag="ACHUQ7_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFR1136982.1"
                     /translation="MITIDGNGAVASVTFRISEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPSG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRQEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACPGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAANLQKRYDFLAQMAGKAEKSNTD"
     gene            complement(4135..>4319)
                     /locus_tag="ACHUQ7_003785"
     CDS             complement(4135..>4319)
                     /locus_tag="ACHUQ7_003785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1136983.1"
                     /translation="LYAFIVTDFIFCWAIALPLIFQHIKRQARTVVRAFFLELDNPYP
                     EHLIGVAFFRFSGHLC"
BASE COUNT         1049 a         1135 c         1123 g         1012 t
ORIGIN      
        1 taacaccatt agcgaaatgc tgaaagaagg tgcacaagtg gatctgaccg cgaaccagtt
       61 aacgctggcg accgcgaaac agacattaac ttataagctg gcggatttaa tgaattaata
      121 gctgccacag ctcccggcgg caagtgactg ttcgctacag cgtttgccgt tgggtaatgc
      181 acacatccca atcgccgtac catccagttg acgggcaaca gaaagcgaac cgccgatcat
      241 tgcacaattt gcttctccac tactggacat cgacgctttt aaacttggcg ctacgtgcgc
      301 cgcagtcgcc tgctgaacag gctcactact acacgccgac aacaataaag cggcacaccc
      361 tacccaaaac gctgctcgca tctcttcttc ctctgatctt caagccaaac gacaccgcca
      421 taaataatag gcagcacaga gggcggcgtc gagagctgtc ctgcgcgttg ccccgcattt
      481 ttactttttt atggctattt ttttgccctc tgtttgatca aaacattcat tactctgatg
      541 tgggggacac aaaagcgaaa atgcagaaga aagccatttg ctaaaattga aggattacta
      601 ctgggcgcgc agcaatttcg tgcgcccctc atttgcgcaa tgtaagggtg tcatatgatt
      661 actattgacg gtaatggcgc ggttgcttcg gtgacgtttc gcatcagtga agttatcgcc
      721 atctacccta ttacccccag ttccacgatg gcagaacagg ctgatgcctg ggccggaaac
      781 ggcttgaaga acgtttgggg agacacacca cgcgtggttg aaatgcagtc ggaagcgggt
      841 gctatcgcta ccgtgcatgg cgctttgcag acgggtgccc tttcaacatc gtttacgtca
      901 tcgcagggtt tgctgctgat gatcccgacg ctgtacaaac tggcaggcga actgacgcca
      961 tttgtcctgc atgtagcggc acgtaccgtt gccacacatg cactttctat ttttggcgat
     1021 cattccgacg ttatggcggt gcgccagacg ggttgcgcga tgttgtgtgc ggctaacgtc
     1081 caggaagcgc aagactttgc tctcatttcg cacatcgcaa cgctgaagag ccgcgtgcca
     1141 tttattcatt tctttgatgg ttttcgcacg tcccacgaaa tcaataaaat tgtcccgctg
     1201 gccgatgaca ctattcttga tctcatgccg caggccgaaa ttgatgctca tcgcgcccgg
     1261 gcgctcaacc cggaacatcc ggtgatccgc ggtacgtccg ccaatcctga cacttatttc
     1321 cagtctcgcg aagccaccaa tccctggtac aacgcggtct atgaccatgt tgaacaggcg
     1381 atgaatgatt tcgctgccgc gacaggtcgt cagtatcagc cgtttgagta ttacgggcat
     1441 ccgcaagcgg aacgggtgat tatcctgatg ggctctgcca ttggcacctg tgaagaagtg
     1501 gttgatgaat tgctaacccg tggcgaaaaa gttggcgtgc tgaaagttcg tctgtaccgt
     1561 cccttctccg ccaaacactt gctgcaagct ctgccgggat ccgtacgcag cgtggcggta
     1621 ctggacagaa ccaaagaacc cggtgcccag gcagaaccgc tctatctgga tgtgatgacc
     1681 gcactggcag aagcctttaa taatggtgag cgcgaaaccc tgccccgtgt cattggtggg
     1741 cgctatggtc tttcatccaa agaatttggc ccggactgtg tactggcggt atttgccgag
     1801 ctcaacgcgg ctaaacctaa agcacgcttt acggttggta tttacgatga tgtgaccaat
     1861 ctgtcactgc cgttgccgga aaacactctg ccaaactcgg cgaaacttga agccttgttt
     1921 tatggccttg gtagtgatgg cagcgtttcc gcaaccaaaa acaatatcaa gattatcggt
     1981 aattccacgc cgtggtacgc acagggctat tttgtttacg actccaaaaa agcgggcggc
     2041 ctgacggttt ctcaccttcg cgtgagcgaa cagccgattc gttccgctta tctcatttcc
     2101 caggctgatt ttgttggctg ccaccagttg cagtttatcg ataaatatca gatggctgag
     2161 cgtttaaaac ctagcggcat tttcctgctt aatacgccgt acagcgcaga tgaagtgtgg
     2221 tcgcgcttgc cgcaggaagt tcaggccgtg ttaaaccaga aaaaagcgcg cttctatgtg
     2281 attaacgcgg cgaaaatcgc ccgcgaatgt ggcctggcgg cccgtattaa taccgtcatg
     2341 cagatggctt ttttccatct gacgcaaatt ctgcctggcg atagcgccct cgcagaattg
     2401 cagggtgcga ttgccaaaag ttacagtagc aaaggtcagg atctggtgga gcgcaactgg
     2461 caggctctgg cgctggcgcg tgaatccgta gaagaagttc cgttgcaacc ggtaaatccg
     2521 cacagcgcca atcgaccgcc agtggtttcc gatgccgccc ctgatttcgt gaaaaccgta
     2581 accgcggcga tgctcgccgg gcttggtgat gccctccccg tttcggcgct gccgccagac
     2641 ggcacctggc cgatgggcac cactcgctgg gaaaaacgca atatcgccga agagatcccc
     2701 atctggaaag aggaactctg tacccaatgt aaccactgcg tcgccgcttg cccacactca
     2761 gctattcgcg caaaagtggt accgcctgaa gcgatggaaa acgcccctgc cagcctgcat
     2821 tcgctagatg tgaaatcgcg tgatatgcgc gggcagaaat atgtcttgca ggtggcaccg
     2881 gaagattgca ccggttgtaa cctgtgcgtc gaagtttgcc cggcgaaaga ccgtcagaat
     2941 ccagagatta aagccatcaa tatgatgtct cgccaggaac atgtcgaaga agagaaaatc
     3001 aattacgatt tcttcctcaa cctgccagaa atcgaccgta gcaaactgga acgtattgat
     3061 attcgtacat cgcagctgat tacaccgctg tttgaatatt caggtgcttg ccccggttgt
     3121 ggcgagacgc cgtatattaa attgctgact cagctctatg gtgaccggat gttgatcgct
     3181 aacgccaccg gctgttcttc aatttatggc ggtaacctgc cctctacacc gtataccacc
     3241 gatgccaacg gtcgtgggcc ggcatgggcg aactctctat ttgaagataa tgccgaattt
     3301 ggccttggtt tccgcctgac ggtcgatcaa caccgtgtcc gcgtgctgcg tctgctggat
     3361 caatttgccg ataaaatccc ggcggaatta ctgacggcgt tgaaatcaga cgctacgcca
     3421 gaggttcgtc gagaacaggt cgcagcttta cgccagcaac tcaacgatgt tgccgaagca
     3481 catgaacttc tgcgtgacgc agatgcactg gtggaaaaat caatctggct gattggtggc
     3541 gacggctggg cttacgatat cggctttggc ggtctggatc atgtattgag tttgacggaa
     3601 aacgtcaaca ttctggtgct ggatacgcaa tgctattcca acaccggtgg tcaggcgtcg
     3661 aaagcgacac cgctgggtgc agtaactaag ttcggcgagc acggcaaacg taaagcgcgt
     3721 aaagatcttg gcgtcagtat gatgatgtac ggtcatgttt atgtggcgca gatttctctc
     3781 ggcgcgcagc tgaaccagac ggtgaaagcg attcaggaag cggaagcgta tccgggacca
     3841 tcgctgatca ttgcttatag cccgtgtgaa gagcatggtt acgatctggc tctcagccac
     3901 gaccagatgc gccaactcac tgctaccggc ttctggccgc tatatcgctt tgatccgcgt
     3961 cgtgccgatg aaggcaaact gccgctggcg ctggattcac gcccgccgtc agaagcactg
     4021 gaagaaacgt tacttcacga gcagcgtttc cgtcgtctga attcgcagca gccagaagtg
     4081 gcagaacagt tatggaaaga tgctgcagct aatttgcaaa aacgctatga cttcctagca
     4141 caaatggccg gaaaagcgga aaaaagcaac accgattaaa tgctctggat aaggattatc
     4201 caattctaaa aaaaaagccc ggacgactgt tcgggcttgt ctttttatat gttggaaaat
     4261 cagtggcaat gcaatggccc aacagaaaat aaaatctgtg acaataaagg catataacc
//
LOCUS       DAZPCS010000225         4209 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZPCS010000225 DAZPCS010000000
VERSION     DAZPCS010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4209
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.230"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..209)
                     /locus_tag="ACHUQ7_003786"
     CDS             complement(<1..209)
                     /locus_tag="ACHUQ7_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR1136984.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="ACHUQ7_003787"
     tRNA            complement(324..399)
                     /locus_tag="ACHUQ7_003787"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="ACHUQ7_003788"
     tRNA            complement(406..480)
                     /locus_tag="ACHUQ7_003788"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="ACHUQ7_003789"
     tRNA            complement(597..681)
                     /locus_tag="ACHUQ7_003789"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="ACHUQ7_003790"
     tRNA            complement(690..765)
                     /locus_tag="ACHUQ7_003790"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="ACHUQ7_003791"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="ACHUQ7_003791"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFR1136985.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACHUQ7_003792"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACHUQ7_003792"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFR1136986.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACHUQ7_003793"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACHUQ7_003793"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFR1136987.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1089 a         1046 c          937 g         1137 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctagaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagtgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc tacacgatga agaaacagcc gaacggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctggcagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttccgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttacagaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgtg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcaagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agctttcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggaactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catgctgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagat atgacgacag gaagagtttg tagaaacgca aaaaggcccg
     4201 actgggctt
//
LOCUS       DAZPCS010000226         4198 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZPCS010000226 DAZPCS010000000
VERSION     DAZPCS010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4198
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.231"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            84..752
                     /gene="ykgH"
                     /locus_tag="ACHUQ7_003794"
     CDS             84..752
                     /gene="ykgH"
                     /locus_tag="ACHUQ7_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgH"
                     /protein_id="HFR1136988.1"
                     /translation="MSEQIKQDIDLIEILFHLKKKIRVILFIMAICMAMVLLFLYINK
                     DNTKVIYSLKINQTTPGILVSCDSNNNFACQTTMTEDVIQRITTFFQTSPDIKNREIR
                     LEWSGDKRDLPTAEEEISRVQASIIKWYASEYHNGRQVLDEIQTPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMVDEYQQ
                     NSGQ"
     gene            complement(995..1690)
                     /locus_tag="ACHUQ7_003795"
     CDS             complement(995..1690)
                     /locus_tag="ACHUQ7_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFR1136989.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1683..3110)
                     /locus_tag="ACHUQ7_003796"
     CDS             complement(1683..3110)
                     /locus_tag="ACHUQ7_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFR1136990.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVASAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(3121..3840)
                     /locus_tag="ACHUQ7_003797"
     CDS             complement(3121..3840)
                     /locus_tag="ACHUQ7_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFR1136991.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAIKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT         1119 a         1085 c          869 g         1125 t
ORIGIN      
        1 ttgctgatat ttaacccgaa cattcctgat acagactatt aatacaacat tatctgcgac
       61 agattttatt ttctggatat attatgagtg aacaaatcaa acaggatatc gatctgattg
      121 agattttatt tcatctgaag aaaaagattc gtgttatcct ttttattatg gctatctgta
      181 tggcaatggt gctgttgttt ctgtatatca ataaagacaa tacaaaagtg atttacagcc
      241 ttaaaataaa ccagacaacg ccaggtatac ttgttagctg tgatagcaat aataattttg
      301 cctgtcagac cacaatgact gaagatgtta ttcagcgaat tactacattt tttcagacca
      361 gcccagatat caagaacaga gaaataaggc tggaatggtc aggagataag agagatttac
      421 caactgctga agaggaaata tctcgcgtgc aggcgtctat tatcaaatgg tatgcgtcag
      481 aataccataa tggcaggcaa gttctcgatg agatacaaac gccttcagca attaacagtg
      541 agctttatac aaaaatgata tacctgacca ggaactggtc attgtatccg aacggtgatg
      601 gctgtgtaac tataagctca ccagaaataa aaaataaata ccctgctgcc atttgcctgg
      661 ctctgggatt ttttctaagc attgtaattt ctgtaatgtt ttgccttgtc aaaaaaatgg
      721 tagatgaata ccaacaaaac tctgggcagt aataaacagc attctgatag agagtgcctg
      781 tataaagctg aagtgacaat gaccgggaag ggaacccgtt ttcaggaatg gatgcctgtc
      841 tttttcactg taaatatcct ggcattacta atgcctggat atttacagaa gagcagcata
      901 accatgacgg aacggggtta agtcaggttg tccttaacgc tattttcaac ctcgtatgcc
      961 ttcttcaggt ttatgttcag atctcatatc tctctcagca atcctcaata atcagataca
     1021 ccgctttcac cggaccatga accccgacga ctttgataag ctcaatatcc gccgttgagc
     1081 tgggaccact gatgatgtta atacatgacg gcattcgttc accggcctgc gctttctgat
     1141 gcaatttttc tgcgagttgc gctacacgcg gcaggattgt gcttttacgc aggataaaaa
     1201 gagaagattc cgggagcagg ctcaatgaac gcccgcgctc ggcggcggaa aaaagaacca
     1261 cgcctcccga ttcggttaaa ccatattcag catacacgac acccacttta gcctgctctg
     1321 cctgcgagat attctcggca cctttcgccg gatcccaaac aacggcattg cattcctgct
     1381 gcaaacgttc actaatcccc aattcctcca gcctcgtgtc accgctaatc acgaccgact
     1441 gatctcccag ctctttacac agacgtattg cagcttctgc cgccttcgcc tcgctggtca
     1501 gctcacagcg cgtcaacata acatcgctgg caaactgaat aaacgcgtca cagcgctgct
     1561 gttggttaag ttgggtaagc cgctcgttag catagttgtt aagcggcgca tcttctgctt
     1621 gcggttcaag tcgcagcggg cgacccagtg cctgagcaac gttattcaaa aattcgctcc
     1681 gattatccat tctttttctc ctgcgcctga tgtttcttaa accaactacg gaaactctct
     1741 ccgtcagctt caggaagatc gcgtgcttcc atccagtcgc taatcgcgcc aaatttgagt
     1801 ggtgttttgc cgccattgat aaaccagctt gccgcatgag cgccggccat catcccgact
     1861 ttccacaatc ctggatgact attggcataa gcgaacattt ttatcgcccg ttgctctgct
     1921 tttgcggtga tccctttttc agccatcacc cgacgatgac gcaaaatcag ttttgacagc
     1981 ggaatacgca ccggacacac gctgtcacaa gcagtgcata aagagcaggc gtagggtaaa
     2041 tctttaaaat ctttatagcc gccaagtagc ggagaaatca ccgcaccaat tggccctgga
     2101 taaatagagc catatccatg accgccaata tggcgatatg ccggacaagt attcatacaa
     2161 gccccgcagc gaatacagcg cagcacatcc cgaaattcag aggccagcac ctcagaacgc
     2221 ccgttatcga caataaccag atgaaactct tcaggaccat caacgtgccc cgcttcgcgc
     2281 ggccctgtca gccaggtgtt gtatcccgtc aaacgtgcac caacggcact gcgcgccagc
     2341 atggtgatca atacatctac ctcggcaaac gtgggggcaa tacgctccat tcccatcact
     2401 gcaatatgcg ttttaggcag cgtggtacac attcgcgcat taccttcatt ggtcaccagg
     2461 cataccgaac cggtctctgc caccgcgaaa ttacagccgg taatacctat ttcagcactg
     2521 aggaaatctt cacggatttt ttgccggatg aataaggtca tcgcttcagg cgtttccggc
     2581 ccctcatagc ccagacgttc gtgtagcact cgacggatct gatggcgatc tttatgaatt
     2641 gccgggacca caacatgaga tggcggatct tgatccagct ggagaatata ttcacccaga
     2701 tcggtttcaa tcacctgaat gccagcatcc tgcaacacat gattgacacc aatctcttcg
     2761 gtcaccatcg atttagattt caccaccttc cgggcatttt tgcgttgggc aacctgtaaa
     2821 atgtagcggg tagcgtcttc tttggttttt gcaaaataga cgtgaccgcc gttttgcgtc
     2881 actttttctg agagctggta cagataagcg tcgagattac tcagaacatg atcacgtatc
     2941 tgggcggccc gatcgcgcca ctcctcccag tgccccaatt catcgaccat tttttgccga
     3001 tttgccccaa tacgctgctg cgcgcttgcc accgctttgc gcatgatcgg atcttcaatt
     3061 tgctgacgga tgcgtgtctt aaaatctgta ttactggttt tgatcgacat ctttatatcc
     3121 tcagcggctc atcaacactt cagcaatatg catcactttg actttctgcc cttcccgttg
     3181 taatcgccca ctgatgttca gcaggcaact cacgtcagca ccaattaaat actcagggcg
     3241 gacttccatc aggtgcacaa ccttttcttt caccatctcg ccggatattt cggccatttt
     3301 gaccgagaac gtgccgccaa atccgcagca ggtatcctgt tcagcaaagg tcaacagctc
     3361 cagtccacgc acatttttca gcagcgtaag tggctcgtcc ttcactccca gcttacgggc
     3421 caggctacaa gatgggtgat acacagctct cccttgcaaa ctggcaccta catcgactac
     3481 ccctaattta ttaacaataa aagaggtgag atcctgcata cgcgcggcaa ccttttcggc
     3541 acgtgatgcc cattcaggtt catccgccag atacgtcggg taacttttta tggcataggt
     3601 gcaagagcca gccggtgaaa taatgggatc gtcgttatcc tccagtgcgg cgatcagatt
     3661 tttcatccct ggaatcgctt ctttgatata accgctattg atcgcaggct gaccgcagca
     3721 tccctgtttc tccgggaaat ttacgcgaca gccgagtttt tccagtagca gcacggagtc
     3781 tcgtgccatt cttgatttca gggcgtcacc aatacaggtg acaaagaaat tgacattcac
     3841 aactaatact ccattacttc atgcccattt atgcccgatg tttccccccc accgggggat
     3901 caatcatcaa taaatatggc gttctttttt aattggctag caacatacca cccctcattt
     3961 gtatgacaaa tgataatctt tcatccaaac ttcaaatgca ataactaatt ttatttcttt
     4021 cggcatcgct cacattttat ggggaaatcg acgatcattt ttggcataag aaatccattt
     4081 aaaaatggct acatccctta tacctattca gagctactta ttcaggggtt attgagatat
     4141 acactgatgg tgtacaaaaa aatcaccgac aataatcacg tatcaagtaa tagtcgga
//
LOCUS       DAZPCS010000227         4183 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZPCS010000227 DAZPCS010000000
VERSION     DAZPCS010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.232"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..190
                     /locus_tag="ACHUQ7_003798"
     CDS             <1..190
                     /locus_tag="ACHUQ7_003798"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1136992.1"
                     /translation="IQSNRDWLSMSIFSDYSSSSEMHNNLTIDYYLALSSTKGSGITN
                     IISIILQQAQDYDVAKIT"
     gene            complement(348..995)
                     /gene="ychE"
                     /locus_tag="ACHUQ7_003799"
     CDS             complement(348..995)
                     /gene="ychE"
                     /locus_tag="ACHUQ7_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFR1136993.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(1026..1079)
                     /gene="ychE"
                     /locus_tag="ACHUQ7_003800"
     CDS             complement(1026..1079)
                     /gene="ychE"
                     /locus_tag="ACHUQ7_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HFR1136994.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            1472..4147
                     /gene="adhE"
                     /locus_tag="ACHUQ7_003801"
                     /gene_synonym="adhC"
     CDS             1472..4147
                     /gene="adhE"
                     /locus_tag="ACHUQ7_003801"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFR1136995.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEDETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         1168 a         1021 c          960 g         1034 t
ORIGIN      
        1 aattcaatca aatagagatt ggctaagcat gtctatcttt tctgattaca gttccagtag
       61 tgaaatgcac aataatttaa caattgatta ttatttagca ctttcctcta ccaaaggcag
      121 tggaattact aatatcattt caattattct tcagcaagcc caggattatg atgtggcgaa
      181 aataacatga tggtgaggag aagcagttaa ggaaatcctt ataactaact gtaatttgag
      241 aagaatattt ttacctattc cttttgcttt tgataactgc gcgggaacaa agtaaaaata
      301 atgacgaagt aaacggagca tggcagctcc gtttcatttg aaaggaatta attaagcagg
      361 ccggggaaaa tccccttaat accagtaacg ataaattcaa tccccaatgc catcagcaat
      421 agccccataa tacgcgtaat cacgttgatg ccggtctggc gtaaaacccg taccagccac
      481 ggtgccatgc ggaacaatcc ccagcaacat aaagcgaaca atgcaatagc cacaaagaaa
      541 ccaaacagat agctaatgct gtgataacgc gtaccccaga cgatggtaga actgatcgcc
      601 cctggccccg ccatcaacgg caacgccaat ggcaccacac caatactttc acgtaccgcg
      661 gtttctgatt tttcttgctt gttctgttta tcctcgccaa gcttgccgct gatcatcgac
      721 atcgctatcg tcaccaccag gatgcccccg gcgatacgga acgaatcaat tgatatacca
      781 aaaagttgta gaatcgtgtc gccgagaaaa agcgagatcc acaagataat ggccacagac
      841 aggttggctg taaggttagt tttgtttcgc gctgccgctg tctgataact ggtcatgctg
      901 ataaagacgg gaataatccc taccgggttg accagcgcaa ataacccgat gaaaaatttg
      961 aagtaaacgg gaaaatcaaa aagggtctga atcacggttc gctccgaagc aaaagccgga
     1021 taatgttagc cataaataag gttgaaaaga cgcgctgaca atacgccttt tgacagcatt
     1081 tttcacctcc taactactta aaattgctat cattcgttat tgttatctag ttgtgcaaaa
     1141 catgctaatg tagccaccaa atcatactac aatttattaa ctgttagcta taatggcgaa
     1201 aagcgatgct gaaaggtgtc agctttgcaa aaatttgatt tggatcacgt aatcagtacc
     1261 cagaagtgag taatcttgct tacgccacct ggaagtgacg cattagagat aataactcta
     1321 atgtttaaac tcttttagta aatcacagtg agtgtgagcg cgagtaagct tttgattttc
     1381 atcggttaag caaatcatca ccgcactgac tatactctcg tattcgagca gatgatttac
     1441 taaaaaagtt taacattatc aggagagcat tatggctgtt actaatgtcg ctgaacttaa
     1501 cgcactcgta gagcgtgtaa aaaaagccca gcgtgaatat gccagtttca ctcaagagca
     1561 agtagacaaa atcttccgcg ccgccgctct ggctgctgca gatgctcgaa tcccactcgc
     1621 gaaaatggcc gttgccgaat ccggcatggg tatcgtcgaa gataaagtga tcaaaaacca
     1681 ctttgcttct gaatatatct acaacgccta taaagatgaa aaaacctgtg gtgttctgtc
     1741 tgaagacgac acttttggta ccatcactat cgctgaacca atcggtatta tttgcggtat
     1801 cgttccgacc actaacccga cttcaactgc tatcttcaaa tcgctgatca gcctgaagac
     1861 ccgtaacgcc attatcttct ccccgcaccc gcgtgcaaaa gatgcaacca acaaagcggc
     1921 tgatatcgtt ctgcaggctg ctatcgctgc cggtgctccg aaagatctga tcggctggat
     1981 cgatcaacct tctgttgaac tgtctaacgc actgatgcac cacccagaca tcaacctgat
     2041 cctcgcgact ggtggtccgg gcatggttaa agccgcatac agctccggta aaccagctat
     2101 cggtgtaggc gcgggtaaca ctccagttgt tattgatgaa actgctgata tcaaacgtgc
     2161 agttgcatct gtactgatgt ccaaaacctt cgacaacggc gtaatctgtg cttctgaaca
     2221 gtctgttgtt gttgttgact ctgtttatga cgcagtacgt gaacgtttcg caacccacgg
     2281 cggctatctg ttgcagggta aagagctgaa agctgttcag gatgttatcc tgaaaaatgg
     2341 tgcgctgaac gcggctatcg ttggtcagcc agcctataaa attgctgaac tggcaggctt
     2401 ctctgtacca gaaaacacca agattctgat cggtgaagtg accgttgttg atgaaagcga
     2461 accgttcgca catgaaaaac tgtccccgac tctggcaatg taccgcgcta aagatttcga
     2521 agacgcggta gaaaaagcag agaaactggt tgctatgggc ggtatcggtc atacctcttg
     2581 cctgtacact gaccaggata accaaccggc tcgcgtttct tacttcggtc agaaaatgaa
     2641 aacagctcgt atcctgatta acaccccggc ttctcagggt ggtatcggtg acctgtataa
     2701 cttcaaactc gcaccttccc tgactctggg ttgtggttcc tggggtggta actccatctc
     2761 tgaaaacgtt ggtccgaaac acctgatcaa caagaaaacc gttgctaagc gagctgaaaa
     2821 catgttgtgg cacaaacttc cgaaatctat ctacttccgc cgtggctccc tgccaatcgc
     2881 gctggatgaa gtgattactg atggccacaa acgtgcgctc atcgtgactg accgcttcct
     2941 gttcaacaat ggttatgctg atcagatcac ttccgtactg aaagcagcag gcgttgaaac
     3001 tgaagtcttc ttcgaagtag aagcggaccc gaccctgagc atcgttcgta aaggtgcaga
     3061 actggcaaac tccttcaaac cagacgtgat tatcgcgctg ggtggtggtt ccccgatgga
     3121 cgccgcgaag atcatgtggg ttatgtacga acatccggaa actcacttcg aagagctggc
     3181 gctgcgcttt atggatatcc gtaaacgtat ctacaagttc ccgaaaatgg gcgtgaaagc
     3241 gaaaatgatc gctgtcacca ccacttctgg tacaggttct gaagtcactc cgtttgcggt
     3301 tgtaactgac gacgctactg gtcagaaata cccgctggcg gactatgcgc tgaccccgga
     3361 tatggcgatt gtcgacgcca acctggttat ggacatgccg aagtccctgt gtgctttcgg
     3421 tggtctggac gcagtaactc acgccatgga agcttatgtt tctgtactgg catctgagtt
     3481 ctctgatggt caggctctgc aggcactgaa actgctgaaa gaatatctgc cagcgtccta
     3541 ccacgaaggg tctaaaaatc cggtagcgcg tgaacgtgtt cacagtgcag caactatcgc
     3601 gggtatcgca tttgcgaacg ccttcctggg tgtatgtcac tcaatggcgc acaaactggg
     3661 ttcccagttc catattccgc acggtctggc aaacgccctg ctgatttgta acgttattcg
     3721 ctacaatgcg aacgacaacc cgaccaagca gactgcattc agccagtatg accgtccgca
     3781 ggctcgccgt cgttatgctg aaattgccga ccacctgggt ctgagcgcac cgggcgaccg
     3841 tactgctgct aagatcgaga aactgctggc atggctggaa acgctgaaag ctgaactggg
     3901 tattccgaaa tctatccgtg aagctggcgt tcaggaagca gacttcctgg cgaacgtgga
     3961 taaactgtct gaagatgcat tcgatgacca gtgcaccggc gctaacccgc gttacccgct
     4021 gatctccgag ctgaaacaga ttctgctgga tacctactac ggtcgtgatt atgtagaaga
     4081 tgaaactgca gcgaaaaaag aagccgctcc ggctaaagct gagaaaaaag cgaaaaaatc
     4141 cgcttaatca gtagcgctgt ctggcaatat aaacggcccc ttc
//
LOCUS       DAZPCS010000228         4182 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZPCS010000228 DAZPCS010000000
VERSION     DAZPCS010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4182
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.233"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..186)
                     /locus_tag="ACHUQ7_003802"
     CDS             complement(<1..186)
                     /locus_tag="ACHUQ7_003802"
                     /inference="COORDINATES: protein motif:HMM:NF017243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HFR1136996.1"
                     /translation="MRHFIYQDEKSHKFWTVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAEKAELKLIAEKVKK"
     gene            complement(327..2360)
                     /gene="metG"
                     /locus_tag="ACHUQ7_003803"
     CDS             complement(327..2360)
                     /gene="metG"
                     /locus_tag="ACHUQ7_003803"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFR1136997.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            2492..3601
                     /gene="apbC"
                     /locus_tag="ACHUQ7_003804"
     CDS             2492..3601
                     /gene="apbC"
                     /locus_tag="ACHUQ7_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFR1136998.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMAGKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            3863..4144
                     /locus_tag="ACHUQ7_003805"
     CDS             3863..4144
                     /locus_tag="ACHUQ7_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFR1136999.1"
                     /translation="MNKYWLSGIIFLAYGLASPAVSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
BASE COUNT         1016 a         1043 c         1062 g         1061 t
ORIGIN      
        1 cttcttcacc ttctccgcaa tcagcttcag ttccgctttt tccgccgccg cagcatctga
       61 aaaacttttt atctgacttt gccctttggt gccaactttt ccccaactga tatgcaactc
      121 gtttccctgt tgctccaccg tccagaattt atgtgatttt tcgtcctgat agataaagtg
      181 tctcattctg catccctata attttatcca gttgaatgca gatgctacca gtatttatac
      241 gggttagaga gagacaaaat cgcaagaaaa caactttagg aatttataaa gaaaaggcgc
      301 tgtcgatgca gcgccttgaa gggggattat ttcacctgat gacccggttt agcaccggca
      361 tccgggctta gcaggaaaat atctttcccg ccaggaccgg cagccatcac catgccttca
      421 gagataccga agcgcatttt acgtggtgcc aggttagcca ccataatggt gtgacgacca
      481 atcagtgcct gcggatccgg gtaagcagaa cgaataccgg agaagacatt gcgtttttca
      541 ccgccgagat ccagcgtcag gcgcagcagt ttgtcagaac cttcaacaaa ctctgcgttt
      601 tcgatcagcg ccacgcgcag gtcaactttg gcgaagtcgt caaaggtgat ggtttcctga
      661 atcggatcat ctgccagtgg gccagtaact ggcgcggtag tggctttcac ttcttcttta
      721 gaggcttcca ccagagcttc aacctgtttc atatcgatgc ggttatacag cgccttgaac
      781 ggattcactt tgtggcccag cagcggttgc tggataccat cccaggtcag ttccgtattg
      841 aggaaggctt ctgcacgctc ggtcagtttc ggcagtacag gcttcaggta agtcatcagt
      901 acgcggaaca ggttgatgcc cattgagcaa atcgcctgca ggtcagcatc gcgaccttcc
      961 tgttttgcga ctacccacgg agcctgctcg tcgacatagc ggttagccag gtcagccagt
     1021 gccatgattt cgcgcactgc tttaccaaat tcacggcttt cccacgcttc accaatcact
     1081 tcagcggcat cggtaaaggt tttgtacagc tcagggtcag ccagctcgct tgccagcaca
     1141 ccgtcaaaac gcttgttgat aaagcccgca ttacgggagg ccaggttaac cactttgttc
     1201 acgatatcgg cattcacacg ctgaacgaaa tcttccaggt tgagatcgat atcatcaatg
     1261 cgcgaagaga gtttcgcagt gtagtagtaa cgcaggctgt cagcgtcaaa atgattcagc
     1321 caggtgctgg ctttaataaa ggtgccgcga gacttggaca tcttcgcgcc gttcaccgtc
     1381 acatagccat gaacaaacag gttggtcggc ttgcggaagt tgctgccttc cagcatggca
     1441 ggccagaaca ggctgtggaa gtaaacgata tctttaccga tgaagtggta cagctcggcg
     1501 gtggagtctt tcttccagta ttcatcgaag cttacgctgt cgccgcgctt gtcgcacaga
     1561 ttcttgaaag aacccatgta gccaatcggt gcgtccagcc agacgtagaa atatttgccc
     1621 ggtgcgttcg gaatttcaaa accaaagtat ggcgcgtcgc gggagatatc ccactgttgc
     1681 aggccggatt caaaccactc ctgcattttg tttgccacct gctcctgcaa cgcaccgctg
     1741 cgggtccatg cctgcagcat ttcgctgaaa gagggcagat caaagaagaa gtgttcagaa
     1801 tcacgcatca ctggtgtagc accagaaacc accgatttcg gctcgatcaa ttcagtcggg
     1861 ctgtaggtcg cgccgcagac ttcgcagtta tcgccgtatt gatccgggga tttacatttc
     1921 gggcaggtgc ctttcacaaa acggtccggc aggaacatgc ctttttccgg atcgtacagc
     1981 tgagagatgg tgcggttttt aataaaaccg ttttctttca ggcgagagta gataagttct
     2041 gacaactggc ggttctcttc gctgtgcgtc gagtgatagt tgtcatagct gatgttaaag
     2101 cctgcgaaat cagtctgatg ctcctgactc atttcgccaa tcatctgctc cggggtgata
     2161 ccgagctgct gagctttcag catgatcggt gtaccgtggg catcgtcggc gcagatgaag
     2221 ttgacctcgt ggccgcgcat tcgctggtaa cggacccaga catcagcctg gatgtgctcc
     2281 agcatatggc cgaggtggat tgagccgtta gcgtacggca gtgcgcacgt caccagaatt
     2341 ttcttcgcga cttgagtcat agtaggcatt acttcttaat agtgaaaagg ggttagatag
     2401 taccaaatgg gaaaatgtta agtaagtact tctctatatt cttcaggtaa actatcagct
     2461 gatggtttat ctatacaaaa aaggagtcgg gatgaacgaa caatcccagg ccaaatcacc
     2521 agaagccttg cgcgcaatgg tcgccgggac actggccaat tttcagcacc caaccctgaa
     2581 gcataacctc accacgctga aagcgttgca ccatgtcgcc tggatggacg acacgttgca
     2641 tgtggaactg gttatgccgt ttgtctggca tagcgcgttc gaagagttaa aagagcaatg
     2701 tagcgccgaa ctgctgcgca tcaccggggc gaaagctatc gactggaaac tgtcgcataa
     2761 catagccact ctgaaacgcg tgaagaatca gccgggaatt aacggtgtga agaacattat
     2821 cgcggtgagt tccggtaaag gcggcgtggg taaatcgtcc acggcggtaa acctggcgct
     2881 ggcactggcg gcggaagggg cgaaagtcgg cattctggac gccgatatct acggcccatc
     2941 aattcccacc atgctgggcg cagaaaatca gcgtccaact tcgccggacg gtacccacat
     3001 ggcaccgatt atgtctcatg gcctggcaac caattcaatt ggttatctgg tcactgacga
     3061 caatgcgatg gtgtggcgtg gaccgatggc gggcaaggcg ttaatgcaga tgttgcagga
     3121 aaccttgtgg ccggatctcg actatctggt gcttgatatg ccgccaggca ccggtgacat
     3181 tcaattgacg ctggcgcaaa acattccagt aactggtgca gtcgtggtca ccaccccgca
     3241 ggacatcgcg ttgatcgatg cgaaaaaagg cattgtgatg ttcgagaaag tcgaggttcc
     3301 ggtgctcggt attgtcgaga acatgagtgt gcatatttgc agtaactgtg gtcaccatga
     3361 accgattttc ggtaccggtg gtgcagagaa actggctgaa aaataccata cacagctgct
     3421 ggggcagatg ccgttacaca tctccttacg tgaggatctc gataaaggca cgccaacggt
     3481 tatcagccgt ccggagagcg aatttaccgc tatctaccgc cagctggctg accgcgttgc
     3541 agctcagctc tactggcagg gtgaagtcat tcctggcgag atttccttcc gcgcggtcta
     3601 acgcctatta accattctgg ttattcttca tacaccgcag agatgttata ttgatgcggt
     3661 gtatttgttt ttgccgcatt aatgaacact tatttaatac tgcgaatatt ctgcggacct
     3721 tcgtccgaaa ctctacaaag tcgagtgatc gtacaggcaa tgcttcatta tcatccttcg
     3781 tgctgtgtag tggatgtaca gctatagttt gttatgcact cattctatcc aggcataacc
     3841 tttactacag ggataacgtt caatgaataa gtattggctg tcaggaatta tttttcttgc
     3901 ctatggattg gcttctcccg cagtctcttc agagactgcc acattaacga ttaatggcag
     3961 gatcagtccc ccaacatgca gtatggctat ggttaatagt caaccacaac agcattgtgg
     4021 tcagttaacg tataatgttg acacccgtca tcaggtttct tcgcccgtca aaggtgttac
     4081 gacagaagtg gttgttgcag gtagcgatag caaacgtcga atcgtgttaa atcgctacga
     4141 ttaatttggc aacctgattt taattgcatt tagaaagacg aa
//
LOCUS       DAZPCS010000229         4168 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZPCS010000229 DAZPCS010000000
VERSION     DAZPCS010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4168
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.234"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..334)
                     /gene="flhe"
                     /locus_tag="ACHUQ7_003806"
     CDS             complement(<1..334)
                     /gene="flhe"
                     /locus_tag="ACHUQ7_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFR1137000.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     ARQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGG"
     gene            complement(334..2351)
                     /gene="flhA"
                     /locus_tag="ACHUQ7_003807"
                     /pseudo
     CDS             complement(334..2351)
                     /gene="flhA"
                     /locus_tag="ACHUQ7_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520726.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(2404..3551)
                     /gene="flhB"
                     /locus_tag="ACHUQ7_003808"
                     /pseudo
     CDS             complement(2404..3551)
                     /gene="flhB"
                     /locus_tag="ACHUQ7_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
     gene            3787..>4168
                     /locus_tag="ACHUQ7_003809"
     CDS             3787..>4168
                     /locus_tag="ACHUQ7_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFR1137001.1"
                     /translation="MEWQQLLNCNRRKDNLKKTNIAFLHPLASGRQEIERDYDRLLFA
                     APTRRLADKTQVFPLDQNDSIRTRLTHSHEVANFARGIGMRLAFEMKEHIFGEIPTGI
                     VVERDVPALLAAIGLAHDLGNPPFG"
BASE COUNT         1038 a         1208 c         1021 g          901 t
ORIGIN      
        1 caccgcctgg cacttcccag ataaatcgca acggttctgc tgccgctata ccggaaaagg
       61 ccacggtggt accgctctgc ccctctaatt cgacacaacg agattgcgaa cacaagcgaa
      121 cccgcagtcc tgcaggtgtc gggccgataa gctgataacg ccacgccacc agcgtcatca
      181 atcctgaagc aggttgtcgc gcagaaagag gtgcagacga catcgactca ccgcgatgat
      241 ttagcgtaat tccgacacta cttgcctgcc acatcccctc cccggcggct tgcaccagca
      301 gcggaaacaa taatatcgct aataaggttc tcatcattta ccgccaattg tcgccgtcat
      361 gcggatatgt cggttatcag acagttccag attcgacagg accactaact gcggcaagct
      421 gcggcgcagg aagcgagaca ataatggtcg cagcgcgtgg ttcaccaaca atactggcgg
      481 cgcacccagc atctcctgac gggatagagc ttcctgagtt tgcgccagta aacgatccgc
      541 cagccctggc tccagtcctc ccccgccctg cagcgcctgt agtaacaaac gttccagcgg
      601 tgtatcgagg ccaataacat ggacttcatc tttgccagga aaccactgct gggtaatcgc
      661 ccgtcccaac gccacgcgca cgacggcgct taattcatgt ggatcgcttt ggatgggcgc
      721 atgttccgcc agcgtttcga gaatggtgcg catatcgcga atcggtactt tttcatcgag
      781 gagattttgc agcactttat gcagtgtggt gagcgtgacg acgccaggaa cgagatcttc
      841 cgtcagcttt ggcatctcct gggcgacgcg atccaacagc tgttgcgcct cctgacgacc
      901 aaacagctct gcggcatgct ggctaatgag gtggttaaga tgcgttgcta ccaccgtgct
      961 ggcctcaacc actgtgtacc cctgaatctg cgcctgttct tttagcgcac tttcaatcca
     1021 gatagcattc aggccaaatg ccggatcgac ggtcgcctca ccaggtaacg tcccggcagc
     1081 ggttccaggg ttaatcgcca gccagcgccc cggataagca tcaccactgc caatctccac
     1141 gcctttcatc aaaatgcgat agcgggcagg ttgcagatcc atattgtcgc gaatgtgcac
     1201 cactggcggc agaaatccca tctcctgggc aaatttcttg cggatactgc gtatacggcc
     1261 caacaactca ccatcctgct ggaaatcgac catcgggatc agtcgataac ccacttccat
     1321 tcccagagaa tcttccagtt gtacatcgtt ccacgtcgct tcgacaacgg tattattctc
     1381 tgccattttt accggtttgg gttcggcagg cgctttttgt tcgcgtccgc gtatccacca
     1441 ggccagcccc agcaatccgg cagtgaacag caaaaatacc aggttcggca ttccaggcac
     1501 caggccgagt aaaccgagca cggcggcgct taacaacata acgcttgggt tactgaaaag
     1561 ctgattcacc atctgctcgc caacatcctg atcggtgctg acacgcgtaa cgatgccccc
     1621 cgcggcggta gaaatcacca gcgccggaat ttgtgccacc agaccgtcgc caatggtcaa
     1681 tagcgtataa ctttccgccg cgtgtcccat gctcatgcca gttgcagcac gccgaccagc
     1741 aacccgccga caacgttaat gaccatgatg aggatcccgg caatggcatc gccgcgaaca
     1801 aacttacttg ccccgtccat tgagccgtaa aaatcggctt cctgagtcac ttcggagcgg
     1861 cgttttttcg cctcatcttc accaatcaat ccagcgttaa ggtcggcgtc aatcgccatc
     1921 tgcttacccg gcataccatc gagaacaaag cgcgcaccca cttctgctat acgcccggca
     1981 cctttggtaa tgaccataaa gttgatgatc acgagaatga caaacaccac gataccgata
     2041 gcgaaattgc caccaacgag gaagtgaccg aacgcttcga ccaccttccc agccgccgcc
     2101 gcgccggtat gcccttccat caaaatgata cgggttgaag ccacgttaag tgccagacgc
     2161 aacaacatag taaacaacag aatggtcgga aacgcagcaa actcaagcgt gcgctgggta
     2221 aacatcgcca ccagcaacac catgatcgac aaggcaatat tgaaggtaaa caacaggtcg
     2281 agtatgaatg cgggcagtgg cagcaccatc atcgacaaga tcaacaggat caaaatcggt
     2341 ccggcaagga tctgccatta tgtcgatttc aggtttgcgg gcaggcgcag catcgcggcc
     2401 agattactca tgggtcggtt tctcgttaat aaaatccagg gctgccggca ccggaagatg
     2461 agtaggtgta cagggcgctg tccaccagcc agacgccagc gtttcagttg ccagacccag
     2521 gccagcactt ccgccaccgc ggcgtacagt tgacccggga tttgttgacc aatctccgca
     2581 tgtcgataca gcgctcgcgc cagcggcggc gcttcaagcg tcgggacgtt attttcagcg
     2641 ccaatttcac gaatgcgcag cgcgaccagc cctgcacctt tagcgaccac tttcggtgcg
     2701 ctcattttgt tttcgtcata ctgcaacgct accgaatagt gggtcgggtt attgacaatg
     2761 acatccgctt tcggcacatc ggccatcatc cgacgccgtg cggcagctcg ctgcatctga
     2821 cagatccgtc ctttaacatg ggggtcgcct tcgctttgtt tgaactcatc acgaatatcc
     2881 tgccgcgaca ttcgcagctt tttcaggtgg ctgaagattt ggaaaaagac gtcaaatccc
     2941 accattggaa tgacaccaag caccaccagc agtgcgcata gccctaccaa atccatcgca
     3001 ttacccatgg cggtaatcgg tgactcggcc attaagcgca tcatctgcgg ccagtgatgc
     3061 cagagaaaaa accccgtcac gctgccaacc aggatggttt tcaaaattgc cttaagcaac
     3121 tccgcgctag tctgtgccga gaatatccgt ttaatgcccg gtagcgggtt gagtttggaa
     3181 aacttcggct gcaaggattt gccgctaaat accagccccc ccagcataac cggagaaatg
     3241 agcgccacca gcaccacgcc gctaatcagc ggtagcagcg ccagcatggc ttctctgatc
     3301 agcagaataa tttgcccgag gatcagattc gggtctttga taatactgtg atcaaaatgc
     3361 agcccagcgg agagcatgcc cgacaatcga cgggccagcg acacaccgcc aaaccagata
     3421 acactaacgc ccaccagcaa aatcagcagt gaggtcagtt cacgggaacg cgggatttgc
     3481 ccctcttccc gcgctttttc tagtcggtga ggtgtggggg cttctgtttt gtcgtcgctc
     3541 tcgtcagaca cgtcgccaat cctggaaaga gtattaagcg taaatgatgc cagagcgcaa
     3601 agcgttcaat ggtttaagta aggggcaaaa caggcgggat ttagggcgtt tatgcaaaaa
     3661 taggttcagt tgcagaacgt tttgcgaacc attttccagt tctgatattt agccatttag
     3721 cctatagaat cgcactccgc ctctctgtta gtttgctaat aattaaatta ttacaaaggt
     3781 gatcaaatgg aatggcaaca attgcttaac tgcaaccgtc ggaaagataa tctaaaaaaa
     3841 accaacatag catttttaca tccccttgct agcggaagac aagagatcga gcgggattac
     3901 gaccgattac tttttgctgc accaactcgt cggcttgcgg ataaaacaca agttttcccg
     3961 ttagatcaaa atgacagcat acggacgagg ctgactcatt ctcacgaagt agcaaacttt
     4021 gctcgcggga taggaatgag gctcgctttc gagatgaagg agcacatctt tggcgaaata
     4081 cccacgggga ttgtcgttga acgcgacgtt cctgctctgt tagcagcgat cggactggca
     4141 cacgatctcg gtaatccacc atttgggc
//
LOCUS       DAZPCS010000230         4114 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZPCS010000230 DAZPCS010000000
VERSION     DAZPCS010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.235"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..158)
                     /locus_tag="ACHUQ7_003810"
     CDS             complement(<1..158)
                     /locus_tag="ACHUQ7_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0001907 - symbiont-mediated killing of host
                     cell [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFR1137002.1"
                     /translation="MDQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYL"
     gene            complement(308..469)
                     /locus_tag="ACHUQ7_003811"
     CDS             complement(308..469)
                     /locus_tag="ACHUQ7_003811"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1137003.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            complement(469..654)
                     /locus_tag="ACHUQ7_003812"
                     /pseudo
     CDS             complement(469..654)
                     /locus_tag="ACHUQ7_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
     gene            914..1249
                     /locus_tag="ACHUQ7_003813"
     CDS             914..1249
                     /locus_tag="ACHUQ7_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFR1137004.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1530..1638)
                     /locus_tag="ACHUQ7_003814"
                     /pseudo
     CDS             complement(1530..1638)
                     /locus_tag="ACHUQ7_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001416037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
     gene            complement(2161..2237)
                     /locus_tag="ACHUQ7_003815"
     tRNA            complement(2161..2237)
                     /locus_tag="ACHUQ7_003815"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2201..2203),aa:Arg,seq:tct)
     gene            complement(2339..2414)
                     /locus_tag="ACHUQ7_003816"
     tRNA            complement(2339..2414)
                     /locus_tag="ACHUQ7_003816"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2379..2381),aa:Ile,seq:cat)
     gene            complement(2559..3380)
                     /locus_tag="ACHUQ7_003817"
     CDS             complement(2559..3380)
                     /locus_tag="ACHUQ7_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR1137005.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPFYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(3395..3751)
                     /locus_tag="ACHUQ7_003818"
     CDS             complement(3395..3751)
                     /locus_tag="ACHUQ7_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001217412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR1137006.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            complement(3764..>4114)
                     /locus_tag="ACHUQ7_003819"
     CDS             complement(3764..>4114)
                     /locus_tag="ACHUQ7_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFR1137007.1"
                     /translation="PQQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYKRWVKTQPCA
                     CCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLI
                     FRFLDHAFATGVLG"
BASE COUNT         1155 a         1118 c          793 g         1048 t
ORIGIN      
        1 agatatgtca gcagcccaaa cagcagactc cccagcacgc ctattgccgc ccactgagac
       61 ggggaaaccc tgtccagcaa ctgcaggaac cagtagcccg ttcccaccgc tgacgtggtg
      121 tatgacacac ctgttgtgat tttttccatc tggtccatac cccgtctccc gttatccgga
      181 agctgacaac aataaaaaag ccaccagtta actactgatg gctctgataa ctcatgcaag
      241 cgtctcagac gacccactga cactaccggt gagtttaacg ataccttcca tttgactggc
      301 tcacttttta tgatgatgcc ggtgcattta tctccagcac cagactttct atctcaacgc
      361 catacgttgc atttttggta atatccgtca gcgtcagtgc atttagtccc actgccagac
      421 tgtcttttat ggcctggaat gccgggccag tacgatgacg tagtatcact ccggctcagt
      481 tgcaccactg accaccacat caccttctgc tgcaatcgcc tgcatcaggg tataaggggt
      541 tatggccacc ggactaccaa acggctgcca gccctctttc agtttatgtg tcagcttttc
      601 cgcaagatct gacggcggcg ccgccctgac aacatcatag tgtttaaatg ccatggttct
      661 ttccaacatc tgaaaaataa ttctttaaaa tacctgacat gtaatacaga aaaaacacaa
      721 aaccatacct taaataaaaa cctgattatc aagcagatat gcatggataa actacaagac
      781 gagatataaa ccaccctgca tttaaataaa caataaacaa catcagaaaa ataattctgt
      841 tctatggttt aattcaaaaa tatcatttat acttttcaga acatcaccag caaggcataa
      901 acaaggaaac taaatgaagt ggattgtgat tgatacagtt atccagccat catgcggaat
      961 atctttttca gtcatatgga gtaaaataaa attaataatc tggtatcaat cggatgcttt
     1021 cttacctcct gaaagtatat ttacactgac tcacacaggc atcatgctca ataacaaagt
     1081 gctacctgta accatttaca acgtagtacc attcaataaa acattctgga atttaatcaa
     1141 aaacagccag gaatgcccta caaatacaga taacgtattg aatgaatgct ttaataaccg
     1201 ttgcactctg caaatatgcc cttatggact aaaacaacaa agtccataag gagtttactc
     1261 acatctgaca aaatcaatat aaacagcccc tccggagagg ggctggagag tggcgctatg
     1321 tgccattcca tggtgccggg tgcctcccgg tgaattcagt accagcacct gaatccgcga
     1381 ttatcccata tacctactcg ctgattgccc ctccgcacag ggggattcac catgccagtt
     1441 tcttttaaca aactccccgc aaaccagaca acagtcaacc gcctgaattg tgaagtattt
     1501 aaaaatttct cccgctaact gatacccggc taacagtctg gcgttttctt tttcagcaac
     1561 gggaaagcaa caaccaccac acccgccacc agcacaccgt cagccggcac tgacattatc
     1621 cggctgctgc aatgccatta acaaaaacag taagcaatca ctttttaccg taacaggtga
     1681 taatccagat atgtatctac cccagatgag taatccgaag ttcatccata ccacaggtcc
     1741 tggctattct gttgtactcc tgaacaagag caaataattc tgaattagca accatgaact
     1801 catcgcaaac cctctgtata gcatcactat tcagaataat aacgtctctt cccgaaagac
     1861 gatcaggagt acagaacaaa actgtcaaac ggctgaaggc ctttgctcgt gctgcattga
     1921 ctatatcaat acgctgccta aggatgaaac accccgacgc ctcatcaata ttcactctac
     1981 ccacaccata tgaatgataa atacttaatg ctgaaaaaac cattagaccg tataacaaat
     2041 actcaatcaa tacttaacag aacttttatt tttgacaaat atataatatt ttcaacaata
     2101 tcctgagcca ggtatatttc agtataaggc tctgccggaa ggaatctgga agaatgaata
     2161 tggcgcgctg tactggattc gaaccagtga ccgattgctt agaaggcaat tgctctgtcc
     2221 ggctgagcta acaacgctga ataccgataa tggaccgcca tcggggaccc gccccccgca
     2281 ccaacaaccc tgttatcgtg tcgtctgctc ttcctgataa gctaatggcg gtttgtgatg
     2341 gtggcccttg ctggatttga accagcgacc tggcgattat gagtcgctcg ctctcaccac
     2401 tgagctaaag ggccgggagc agaataataa cggtccgtaa ttaatttcgc aataaaaaaa
     2461 cccgctcagc ggcgggttgt agaaactctt ctaacgccag gcataaaacg cccatcatta
     2521 tgacgaattt accacagatt ccggaaaaat caaccttgtt acctagttac cttttttaac
     2581 tgccgctcag cccatgcttc ttcaatatca aaccgggtca ccagcgcatc ataaaatttc
     2641 ttaactgttt tttcccatga cgcgcgtgtt atctggtttg tcacctcgca tatagcatta
     2701 aatgcctccg ttgatggtag tctttcatag ccacgaccac cacaacgctg gcagtctctg
     2761 ataacaggca taccacgttt taccgactct tcacgatgaa tggcgacacc acgcccacgg
     2821 caatccttac aggcggtgga aacctcaccc tttccgccac actccggaca ggcaactttt
     2881 accacctccc tgactttttt ccattcttcc cagtaaaacg gatacacacc tttcgtacac
     2941 tttgcccata ccggcggctt accatccgga tactggatct tgtttgtaaa aactatgctt
     3001 tcaataaatt tttccccata gcaacaaggg cactgctttt tactcgctgc gctgcgggca
     3061 taatcctcaa aagcgtacga agccataatg cgcatcacta ccggttttat ttctgccgga
     3121 agttttctca acgccgccac acgatcgcac cgactgagtg cataatctgc cagtaattct
     3181 gttgcccgcg ccctgtcatt catactgatg cccattttcc ccaggaacgc agaaaaaccc
     3241 atctcagccc gattctgtgt catgccctgc gcggccatca catcagtgat actcagcgca
     3301 tcttttgacg ttgaggccga tgcatcggtc aggccagggg attttgggga gtagtatttc
     3361 ggtaaatctt ccagtttcat tttttgacct gctcttaatg cattatgggg taaatcttca
     3421 cccccagacg tccaccagat actggctgac cacgaacgat attgatttca tcaaactgct
     3481 catcgtccat taacactccc gcatgcgtca gcgcatccag cggtgctttc aggatattgt
     3541 ccaggtcgcg acgacgctta tccggtggct ctgcaatcac ctttatcgcc agccttccgg
     3601 acaggcttaa tttcagccgc tgctggcgaa caataagcgc cacagcccgg cgataacgct
     3661 ttccctcctc cgagataaaa tatgtgctgc cacggcgtcg ccagtaagtg ttcaccgtcg
     3721 gcgggtaagg taaaaccaaa tctatgagca tcagtcacct cttttaccca agcacgccag
     3781 ttgcaaaggc gtgatcaaga aaacgaaaaa ttaaatcaac ctgagaacca tgcttttctt
     3841 cgaacgccag aggatccgca tgaagctcgt tgtgatgctc ccgacacagc ggtagcgtga
     3901 aaatatcgtg agatttggtc cccattccgc cctgaccatg accaatcagg tgatggggat
     3961 cgtcggctgg cttaccacaa cacgcacacg gctgtgtctt cacccagcgt ttgtatttct
     4021 cgttaaccca gcggcgacgt ttaggtcgtt tcatgaaaga ttccggagac tcaggatcaa
     4081 cggcaatgct gaccaccgtc ttttcctgtt gtgg
//
LOCUS       DAZPCS010000231         4076 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZPCS010000231 DAZPCS010000000
VERSION     DAZPCS010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4076
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.236"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            131..334
                     /gene="aaeX"
                     /locus_tag="ACHUQ7_003820"
     CDS             131..334
                     /gene="aaeX"
                     /locus_tag="ACHUQ7_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFR1137008.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            342..1274
                     /gene="aaeA"
                     /locus_tag="ACHUQ7_003821"
     CDS             342..1274
                     /gene="aaeA"
                     /locus_tag="ACHUQ7_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFR1137009.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSDLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            1280..3247
                     /gene="aaeB"
                     /locus_tag="ACHUQ7_003822"
     CDS             1280..3247
                     /gene="aaeB"
                     /locus_tag="ACHUQ7_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFR1137010.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTHFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            3339..3611
                     /locus_tag="ACHUQ7_003823"
     CDS             3339..3611
                     /locus_tag="ACHUQ7_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFR1137011.1"
                     /translation="MNIYAFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(3667..3930)
                     /gene="yhcN"
                     /locus_tag="ACHUQ7_003824"
     CDS             complement(3667..3930)
                     /gene="yhcN"
                     /locus_tag="ACHUQ7_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFR1137012.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT          957 a          988 c         1071 g         1060 t
ORIGIN      
        1 aacagttacg ctaatatatt aaatatcaat ctacagcaat gttgctctcg cccggctttc
       61 catgcccttc tctgcggcga cagatgctga aaataataac gcctgctctc tctatacaac
      121 caaggtcaac atgagtctgt ttcccgttat cgtggtgttt gggctgtcct tcccaccgat
      181 attttttgaa ttgcttttat cactggcgat tttctggctg gtgcgccggg tacttgtgcc
      241 aacaggtatc tacgactttg tctggcatcc ggcgttgttc aacaccgcgc tctattgctg
      301 cttgttttat ttgatatcgc gactgttcgt ttgaggttga agtgaaaaca ctaataagaa
      361 aattctcccg tacggccatc acggtcgtat tagtcattct ggccttcatc gcaattttta
      421 atgcctgggt ctattacacc gaatccccct ggacgcgtga cgcgcgcttt agcgctgacg
      481 tcgttgcgat cgcgccggac gtttctggac tcattaccca ggtgaatgtt catgataacc
      541 agctggtgaa aaaaggacag atactgttca ccatcgacca gccgcgctat caaaaggcgc
      601 ttgaggaagc gcaagccgat gttgcttatt atcaggtact ggcacaggag aaacgccagg
      661 aggccggacg tcgtaaccgt ctcggtgtgc aggcgatgtc tcgcgaagag atcgaccagg
      721 ctaacaacgt actacaaacg gttctgcatc agttagcgaa agcgcaggcg acccgcgatc
      781 tggcaaaact ggatcttgaa cgcacggtga tccgagcgcc agcagatggc tgggtgacca
      841 acctcaacgt ctataccggt gagtttatta ctcgaggatc aacggcggtt gcgctggtga
      901 aacagaactc cttctatgta ctggcctata tggaagaaac taagctggaa ggggtgcgtc
      961 cggggtatcg tgcagagatc acgccgcttg gcagtaacaa agtgctgaaa gggactgttg
     1021 atagtgttgc cgctggggtc accaacgcca gcagcacgcg tgacgacaaa gggatggcga
     1081 ccatcgactc tgaccttgaa tgggtgcgtt tggcgcaacg tgttccggtt cgtattcgtc
     1141 tcgacaacca gcaagagaac atctggcctg cgggcaccac tgctacagtg gtggtcactg
     1201 gcaaacaaga tcgcgacgaa agccaggatt cgttcttccg taaaatggcc catcgcctgc
     1261 gtgagtttgg ttaatcacga tgggtatttt ctccattgct aaccaacata ttcgctttgc
     1321 ggtaaaactg gcgaccgcca ttgtactggc gctgtttgtt ggctttcact tccagctgga
     1381 aacgccacgc tgggcggtac tgacagcggc gattgttgcc gccggtccgg cctttgctgc
     1441 gggaggtgaa ccgtattctg gcgctattcg ctatcgtggc tttttgcgca tcatcggcac
     1501 atttattggc tgtattgccg gactggtgat catcattgcg atgatccgcg caccattatt
     1561 gatgattctg gtgtgctgta tctgggccgg tttttgtacc tggatatcct cgctggtacg
     1621 aatagaaaac tcgtacgcgt gggggctggc cggttatacc gcgctgatca ttgtgatcac
     1681 cattcagccg gaaccattgc ttacgccgca gtttgccgtc gaacgttgta gcgagatcgt
     1741 tatcggtatt gtgtgtgcga ttatggcgga tttgctcttt tctccgcgat cgatcaaaca
     1801 agaagtagat cgagagctgg aaagtttgct ggtcgcgcaa tatcaattaa tgcaactctg
     1861 tatcaagcat ggcgatggtg aagttgtcga taaagcctgg ggcgacctgg tgcgacgcac
     1921 cacggcacta caaggcatgc gcagcaacct gaatatggaa tcttcccgct gggcgcgggc
     1981 caatcgacgt ttaaaagcga tcaatacgct atcgctgacg ctgattaccc aatcctgcga
     2041 aacttatctt attcagaata cgcgcccgga attgatcact gatactttcc gcgaattttt
     2101 tgacacgccg gtagaaaccg cgcaggacgt ccacaagcag ctcaaacgcc tgcggagagt
     2161 tatcgcctgg actggggaac gggaaacgcc tgtcaccatt tatagctggg tcgcggcggc
     2221 aacgcgttat cagcttctca agcgcggcgt tatcagtaac acaaaaatca acgccaccga
     2281 agaagagatc ctgcaaggcg aaccggaagt aaaagtagag tcagccgaac gtcatcatgc
     2341 aatggttaac ttctggcgaa ccacactttc ctgcattctg ggcacgcatt tctggctgtg
     2401 gacgggctgg acttccggca gtggtgcaat ggtgatgatt gcggtcgtga cgtcactggc
     2461 aatgcgtttg ccgaatccac gcatggtggc gatcgacttt atctatggga cgctggccgc
     2521 gctgccgtta gggctgctct actttttggt gattatccct aatacccaac agagcatgtt
     2581 gctgctgtgt attagcctgg cagtgctggg attcttcctc ggtatagaag tacagaaacg
     2641 gcgactgggc tcgatggggg cactggccag caccataaat attatcgtgc tggataaccc
     2701 gatgactttc catttcagtc agtttctcga cagcgcatta ggacaaatcg tcggctgtgt
     2761 gctcgcgttc accgttattt tgctggtgcg ggataaatcg cgcgacagga ctggacgtgt
     2821 actgcttaat cagtttgttt ctgccgctgt ttccgcgatg actaccaatg tggcacgtcg
     2881 taaagagaac cacctcccgg cactttatca gcagctgttt ttgctgatga ataagttccc
     2941 aggggatttg ccgaaatttc gcctggcgct gacgatgatt atcgcgcacc agcgcctgcg
     3001 tgatgcgccg atcccggtta acgaggattt atcggcgttt caccgacaaa tgcgccgcac
     3061 agcagaccat gtgatatctg cccgtagcga tgataaacgt cgtcggtact ttggccagtt
     3121 gctggaagaa ctggaaatct accaggaaaa gctacgcatc tggcaagcgc caccgcaggt
     3181 gacggaaccg gttcatcggc tggcggggat gctccataag tatcaacatg cgttgaccga
     3241 tagttaagtc aaaaccgacg ccaaaagcgt cggttttttc atggctatac ttagcgatgc
     3301 acggcagaac tcgccgcgaa acgtgacggt ggcaacagat gaatatttat gcctttgatt
     3361 ttgatgagat tgagagtcag gaggattttt atcgtgactt tagccaaacc tttggtctgg
     3421 ctaaagataa ggtacgcgat ctcgactcac tatgggatgt gttaatgaac gatgtcctgc
     3481 cgctaccact tgagattgaa tttgttcatc tgggagagaa aacgcgtcgc cgttttggcg
     3541 cgttaatatt gctgtttgat gaggcagagg aagagctgga agggcatttg cgttttaatg
     3601 ttcgtcatta gcgcaaaaaa aagcccacga accgggggca atatcgtcgg acaaaacgat
     3661 gagggtttat ttgtacagtt cagccgtagc gtgccaggtg tcaccgctac gagcttcagt
     3721 aatctggtag gccgttgcgc ctttctcttc cgcttttttg ttcagcattt cacgtatatc
     3781 cattggcgaa gacgccacac cacttacgga tacggtcccg attgcttcac gattttgtgc
     3841 ttgtgcagca tcaatggagt cggcagcgaa tgcaccgaaa gagagaacag aaagtacgct
     3901 taatgcagct acagtggttt tgattttcat gatttttacc tcgacataat cttttagctg
     3961 ggcctttgtt tcgtgaccca tatcacaaaa tcaagtatac actaattatt gaactaatta
     4021 ataccacact aactgtttca gttgatacta agtgttaaaa atgtgaattt taataa
//
LOCUS       DAZPCS010000232         4064 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZPCS010000232 DAZPCS010000000
VERSION     DAZPCS010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.237"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..662)
                     /locus_tag="ACHUQ7_003825"
     CDS             complement(<1..662)
                     /locus_tag="ACHUQ7_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AadA family aminoglycoside
                     3''-O-nucleotidyltransferase"
                     /protein_id="HFR1137013.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QY"
     gene            complement(720..1244)
                     /gene="sat2"
                     /locus_tag="ACHUQ7_003826"
     CDS             complement(720..1244)
                     /gene="sat2"
                     /locus_tag="ACHUQ7_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HFR1137014.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            complement(1339..1812)
                     /gene="dfrA1"
                     /locus_tag="ACHUQ7_003827"
     CDS             complement(1339..1812)
                     /gene="dfrA1"
                     /locus_tag="ACHUQ7_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HFR1137015.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            2144..3121
                     /gene="intI2"
                     /locus_tag="ACHUQ7_003828"
                     /pseudo
     CDS             2144..3121
                     /gene="intI2"
                     /locus_tag="ACHUQ7_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            complement(3309..3548)
                     /locus_tag="ACHUQ7_003829"
     CDS             complement(3309..3548)
                     /locus_tag="ACHUQ7_003829"
                     /inference="COORDINATES: protein motif:HMM:NF020425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA family protein"
                     /protein_id="HFR1137016.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
BASE COUNT         1092 a          941 c          886 g         1145 t
ORIGIN      
        1 tgatactggg ccggcaggcg ctccattgcc cagtcggcag cgacatcctt cggcgcgatt
       61 ttgccggtta ctgcgctgta ccaaatgcgg gacaacgtaa gcactacatt tcgctcatcg
      121 ccagcccagt cgggcggcga gttccatagc gttaaggttt catttagcgc ctcaaataga
      181 tcctgttcag gaaccggatc aaagagttcc tccgccgctg gacctaccaa ggcaacgcta
      241 tgttctcttg cttttgtcag caagatagcc agatcaatgt cgatcgtggc tggctcgaag
      301 atacctgcaa gaatgtcatt gcgctgccat tctccaaatt gcagttcgcg cttagctgga
      361 taacgccacg gaatgatgtc gtcgtgcaca acaatggtga cttctacagc gcggagaatc
      421 tcgctctctc caggggaagc cgaagtttcc aaaaggtcgt tgatcaaagc tcgccgcgtt
      481 gtttcatcaa gccttacggt caccgtaacc agcaaatcaa tatcactgtg tggcttcagg
      541 ccgccatcca ctgcggagcc gtacaaatgt acggccagca acgtcggttc gagatggcgc
      601 tcgatgacgc caactacctc tgatagttga gtcgatactt cggcgatcac cgcttccctc
      661 atgatgttta actcctgaat taagccgcgc cgcgaagcgg tgtcggcttg aatgaattgt
      721 taggcgtcat cctgtgctcc cgagaaccag taccagtaca tcgctgtttc gttcgagact
      781 tgaggtctag ttttatacgt gaacaggtca atgccgccga gagtaaagcc acattttgcg
      841 tacaaattgc aggcaggtac attgttcgtt tgtgtctcta atcgtatgcc aaggagctgt
      901 ctgcttagtg cccacttttt cgcaaattcg atgagactgt gcgcgactcc tttgcctcgg
      961 tgcgtgtgcg acacaacaat gtgttcgata gaggctagat cgttccatgt tgagttgagt
     1021 tcaatcttcc cgacaagctc ttggtcgatg aatgcgccat agcaagcaga gtcttcatca
     1081 gagtcatcat ccgagatgta atccttccgg taggggctca cacttctggt agatagttca
     1141 aagccttggt cggataggtg cacatcgaac acttcacgaa caatgaaatg gttctcagca
     1201 tccaatgttt ccgccacctg ctcagggatc accgaaatct tcatatgacg cctaacgcct
     1261 ggcacagcgg atcgcaaacc tggcgcggct tttggtacaa aaggcgtgac aggtttgcga
     1321 atccgttgct gccacttgtt aacccttttg ccagatttgg taactataat ttatgttaga
     1381 ggcgaagtct tgggtaaaaa ctggcctaaa attgctgggg atttcaggaa agtaaacatc
     1441 accttccggc tcgatgtcta ttgtagatat atgtagtgta tctacttgat cgatcaggct
     1501 tttgtatatc tccccaccac ctgaaacaat gacatgatcc gttattttct ttaggttggt
     1561 taaagcatct ttaattgatg gaaagatcaa tacgttctca ttgtcagatg taaaacttga
     1621 acgtgttacg accgcatact ttcggttggg taatgctccc attgattcaa aagtcttgcg
     1681 tccaaccaac agccattggt tataggtaat agctttaaac aggagctgtt cacctttggc
     1741 actccatgga atatcagggc cattcccgat aactccattc ttcgatatag ctaccattag
     1801 tgatagtttc acaattcttc ctcagaggtt aacattttat tagtaagcat gctcgttttg
     1861 tcacccgctg atgcttaccg ttaattaata taatcacttg tattaaatag actttcttta
     1921 aaaatacaag acactctgtt attacaaatc gtgcatgccg tctatcctgc ttgcacgatg
     1981 cacattatct cacccaaaga actcataaca ttttaattta tttagcatta aattaaaact
     2041 ctaccggtaa acaagcatct ctaggcgtaa aaaacaggca cttttttaaa tcccagctag
     2101 aataggctgt ataggcagac agttgcaaga caaggagcgt tttatgtcta acagtccatt
     2161 tttaaattct atacgcacgg atatgcgaca aaaaggttat gcgctgaaaa ctgaaaaaac
     2221 ttacctgcac tggattaagc gttttattct gtttcacaaa aaacgtcatc ctcagaccat
     2281 gggcagtgaa gaggtcaggc tgtttttatc cagcttagca aacagcagac atgtagccat
     2341 aaacacgcag aaaatcgctt taaatgccct agcttttttg tacaacaggt ttttacaaca
     2401 gccgttgggc gatattgatt atatccctgc aagcaagcct agacggctac cctctgttat
     2461 ctctgcaaat gaagtgcaac gcattttgca ggttatggat actcgcaacc aagttatttt
     2521 tacgctgctg tatggtgcag gtttgcgcat taatgaatgc ttgcgtttgc gggttaaaga
     2581 ttttgatttt gataatggct gcatcactgt gcatgacggt aagggtggga aaagcagaaa
     2641 cagcctactg cccacgcgcc taatcccagc aataaaataa ctcattgagc aagcgcggct
     2701 tattcagcaa gacgacaact tacaaggcgt agggccatcg ctgccttttg ctttagatca
     2761 caaataccct tctgcttatc gacaagcggc gtggatgttt gtctttccct ccagcacgct
     2821 ctgcaaccac ccgtataacg gcaaattatg ccgccatcat ctgcatgact ccgttgcgcg
     2881 aaaggcattg aaggcagccg tacaaaaagc aggcatcgtt agcaagcgtg tcacttgtca
     2941 tacatttcgt cactcgtttg ctacgcatct attacaagcg gggcgtgata ttcgcactgt
     3001 gcaagaactc ttagggcata acgatgttaa gaccacgcaa atctatacgc atgtgttggg
     3061 tcagcatttt gccggcacca ccagtcctgc ggatggactg atgctactta tcaatcagta
     3121 aaagtagata aatatcttta tgtagccctg agtagcccct gatacttttt atggtgaaaa
     3181 agataaacaa tcatagaggg gataaatatg agccagttct accattgttc agataagttt
     3241 aagcaagata ctgtcaatca cagtgaccat tcatggtcat tacacttcct ccagttgcca
     3301 gacaatgcct aacataaaaa cgcaaaagcc caaaagtcag agcttaggtg ttctaggcgt
     3361 tttctaagcg ctgaagaaat acgtcttacc gtagattgcg tgcgtttctg aagcacattt
     3421 tccttataca tggctttatc ccaggcagct tcatcaagct cttggagtaa caagccagta
     3481 ataatgcgac actctcgggt ttgtagcgag ccgctggtga gatcggagtt atagcggaaa
     3541 ggggtcattt gtaattattt gtgggtggag gatagttaac ttattattgt agttggaaga
     3601 ggctgctttc caaacattag cacctcgtaa tgcttgttac tgtatattaa tttatgctct
     3661 tagcgcatgt taatgggcta ttacaatgca taatgtccac atcaagataa ctgtggctta
     3721 acacctgtac aagcgaaaaa aatcaatcac ctgtccggaa atacgggatc actgtaaagt
     3781 gttggtgagg cagtctagga gggggattta atttgggata ctgatgaaag gattatgatc
     3841 agttggatat aggatcgtac taagctgagc ggcaggttag ctagattagt taaccagata
     3901 agtgaaatct agttccaaac tattttgtca tttttaattt tcgtattagc ttacgacgct
     3961 acacccagtt cccatctatt ttgtcactct tccctaaata atccttaaaa actccatttc
     4021 cacccctccc agttcccaac tattttgtcc gcccacagct gccg
//
LOCUS       DAZPCS010000233         4045 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZPCS010000233 DAZPCS010000000
VERSION     DAZPCS010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.238"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(267..1124)
                     /gene="sitD"
                     /locus_tag="ACHUQ7_003830"
     CDS             complement(267..1124)
                     /gene="sitD"
                     /locus_tag="ACHUQ7_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HFR1137017.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1121..1978)
                     /gene="sitC"
                     /locus_tag="ACHUQ7_003831"
     CDS             complement(1121..1978)
                     /gene="sitC"
                     /locus_tag="ACHUQ7_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HFR1137018.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(1975..2802)
                     /gene="sitB"
                     /locus_tag="ACHUQ7_003832"
     CDS             complement(1975..2802)
                     /gene="sitB"
                     /locus_tag="ACHUQ7_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HFR1137019.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(2802..3716)
                     /gene="sitA"
                     /locus_tag="ACHUQ7_003833"
     CDS             complement(2802..3716)
                     /gene="sitA"
                     /locus_tag="ACHUQ7_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HFR1137020.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
BASE COUNT         1102 a          986 c          978 g          979 t
ORIGIN      
        1 gagctgaagg aagagctgaa tagtctgaag gatttgaatt cggttaggaa ttttatcgtg
       61 gaataacaga agctgcggca cgtcgtatgc aggaacgtgc cgcggttggc tggtaaactt
      121 tcgatagtgc gagtattgaa tgatttctag gcgttatcga ttttacgtat tttttgcatg
      181 atagaattcg tacctcctcc caacgacctt ccatggcttt ccggacttct gtaggctggc
      241 ggacgatgct gtcctcggat gttgtgctaa ttgggtgata acagatcctg tgtatgggcg
      301 tttccctttg tgcgtgcgtt gatgctggta acggtaaacg tcatgatgaa cacgaccgcg
      361 aataagacga caatcgtcgg agctggtgcg ctgtcgataa aaaaggacag ccagacacca
      421 gtcattgaca ctattaccga tatgccagtc gccagtagga gcgcaatatg aaagcgttgt
      481 gtcaggagta ctgcaatggc gccaggcgca atgagcagag aaatagagag aatgatacct
      541 actgctttca gcgtcgccac aatggtcagg gaaaccatgc acagcagccc ataatgcagc
      601 catctcgtat gcaaaccgct tacctgcgcc tgttgataat caaagctgaa cagcaaaaaa
      661 tcccgccatt ttacgctaat aacaagtgta accagcccgg caataatcac cgtctggatt
      721 atgtcgccga tggttatccc gagcatgtcg ccgaaaagaa tatggtcaag atgtacgtct
      781 ggtttaattg caatatacaa gatcagacct gcggcaaaca tgccagaaaa aacaatcccc
      841 ataactgtat cttgcttgat tcggctgttg tctttcaggt atcccgtcgc caccgcgcag
      901 aatactccgg cgacgaatgc ccctgtagcc agaggtaacc ccagaatcca ggtaagcacg
      961 atgccaggaa aaaccgcatg actcatcgcg tcacccatca gtgcccaacc tttcaggacc
     1021 agaaagaccg acagcagcgc acagggtatg gaaactacca gtgaaatcag cagtgcgtga
     1081 ctcataaagg tgaactgcag tggctccagt aataatgcca tcatgagcga tcctccagcg
     1141 ctttatgcgc acgccgacga tttgccaaaa gtccatgggt gggcgcaaat acaaatgcta
     1201 aaagaaacag cagtgtctgc gccaccacga tgattccacc tgtcgcgccg tccagaaagt
     1261 aactgaccca cgcgccgagg aaactggtta cactgccaat agttacagca attatgagca
     1321 ggcggggaaa acgatcggtt aacaaccatg ctgttgcacc gggagtcacc accagacaga
     1381 tcaccagaaa ggcaccaacc gtttgcagcg ccgcaacggt cgaaaccgac agcagggtga
     1441 agaagaggat ttttaatctg cccggatgta atccaatagc acgggcgtga ttctcgtcga
     1501 aaaaggtcac cataagatct ttccatttga aaaaaagcac gatgatcgag agaatgccaa
     1561 tgatagtgag ttgcaggata tccgccggat cgattgccag aatattgccc aggacgatgg
     1621 tctgaatgtt cacggatgtc gggttcaatg acaccataaa cagccccagg ccaaaaaagg
     1681 acgagaagat caggccgatg atggcatctt cttttaagcg ggtgcgttgg ttaagaaaca
     1741 gcatgctgcc cgctgccagt ccgccggaaa agaaggctcc cagagaaaac ggaagcccca
     1801 gcatatatgc ccccgcaaca cccgggacaa tagagtgcga cagcgcatcg ccaatcagcg
     1861 accagccttt tagcatcaga tagcatgaca ggaaagcgca gagaccgccg accattgccg
     1921 agacccacat cgcattgagc atatattcat agctgaaggg ttccagtagc acgttcatct
     1981 tgcttccctc tgcaccgctg acggtcgatg tgccacaaaa gggcgttcat cgtcagtaat
     2041 aatgctttct tccgagccgt taagtgtgac atggcgcagt acgccgctaa aagccagctc
     2101 caggttctcg gctgtaaaag tggtgtctgt cggcccgctt gccaggacgg tgcctttgac
     2161 catgaccgta taatcgcaaa atgtcgtgac tgagccgaga ttgtgcgttg agacaagcat
     2221 cgttttgcct tccgcgcgta actccctcaa caggctgatg attttcgctt cggttttcac
     2281 atcaacgccg gtaaaaggtt catccagcag gatcacatca ccctgctgtg caattgctct
     2341 cgccagaaag acgcgtttct tttgtccacc ggaaagctcg ccgatttgac gatggcgaaa
     2401 atccaccata tcgacacgtt ccagcgcatc ggtgacaatc tgacgatccc tctttttggc
     2461 tatacgcaac atacccatat gcccatagcg gcccatcatc accacatctt ccaccagtac
     2521 aggaaatgac cagtcaacct cttctgactg agggacgtag gcaaccaggt ttttctgtag
     2581 cgcctgtcgc gtgggaatac ccagaacaga tatctttccg ctggtcagac gcacaaatcc
     2641 cataatcgct ttaaacaacg tcgattttcc ggaaccgttt accccaacca gagcggcaat
     2701 agatccactt ggtactgtga aggatgcatc gcgcagtgct gtgtgcccgt tacgccaggt
     2761 gacagtaaca tcattaacga caatgcctgc agattgcatc attatttctc ccttttcccg
     2821 gctttaattc cctgtaccag cgtactggta gtaaccttaa gaaggtcgat atacgtaggt
     2881 accgggccgt tttctgtgct cagggaatcg acatagagca caccgccgta gtgtgcgccg
     2941 gtttcacgcg caacctgacg cgctggttta tcggaaatcg tactctcgct aaagactgcc
     3001 gggatatgat ttttcttaac tatatcaaca accttacgta cctgctgcgg tgttccttgt
     3061 tgatcggcat taatcggcca cagataaagc tcttttagcc ccaaatcgcg tgcgagataa
     3121 gaaaaagccc cttcactggt gaccatccat cgctgattct cagggagttc cgtaatctgc
     3181 ttacgcaggg gggcaagggt ttgggtaatc ttggctttat aagtatcggc attacgttgg
     3241 taggtttgtg catttgccgg atcgtatttt atcaacgcat cacgaatatt atcgacgtaa
     3301 atcagagcat tatctggcga catccatgca tggggattag gtttgccctc atagggtcct
     3361 tcggtgatcc ctactggcgt cacacccgaa gagacaatta cttctggaac cccattgaga
     3421 tgctggtaaa agcgttggaa ccacaattcc agattcatac cattggcgag aatcagttgt
     3481 gccccctgcg cacgtttaat atcgccaggg gtaggctgat actcatgaat ttctgcacca
     3541 ggcttggtta tggatgagac ttctgcagca tctccagcca cgtttttggc catatccgcg
     3601 atgatggtaa atgttgtaat gaccttgaat ttttcagttg cacttgcctg aaatgcgatc
     3661 gagcaggtga gggcgaggca ccccaagagc atggttactt tttttattga gtgcataatt
     3721 tagtacctat agtgagcaaa attagaacaa acatagcaac ggctatgttt tatagcataa
     3781 cctatacaaa atgaaaataa ttatcattaa cagctttgag gcctaaaaca gggattaggt
     3841 tgtttcctgc tggcgacatg atattgagga aaaaagtaga gcagctatta ggctttgaaa
     3901 tgactttatg aggaaaattg ttgatggcaa aaatggggca aaaaagagtg ggttatcgta
     3961 gcttattgtt gtcgctgata atgtttaaca tattgaaaaa taaataaaat acttatgagt
     4021 cagatagttg tgatttttgc cctta
//
LOCUS       DAZPCS010000234         4042 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZPCS010000234 DAZPCS010000000
VERSION     DAZPCS010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.239"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(126..284)
                     /locus_tag="ACHUQ7_003834"
     CDS             complement(126..284)
                     /locus_tag="ACHUQ7_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR1137021.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAIGNWTRWIEI
                     LRVLEVVA"
     gene            complement(886..1077)
                     /locus_tag="ACHUQ7_003835"
     CDS             complement(886..1077)
                     /locus_tag="ACHUQ7_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFR1137022.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLYDH
                     ADELYRSCLARFGDDGENL"
     gene            1142..1465
                     /locus_tag="ACHUQ7_003836"
     CDS             1142..1465
                     /locus_tag="ACHUQ7_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFR1137023.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPT
                     LAPPLLRQLAAIGNNLNQAARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            1674..2105
                     /locus_tag="ACHUQ7_003837"
                     /pseudo
     CDS             1674..2105
                     /locus_tag="ACHUQ7_003837"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2143..2673
                     /locus_tag="ACHUQ7_003838"
     CDS             2143..2673
                     /locus_tag="ACHUQ7_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HFR1137024.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLSDEFRKLEESVSKAVTSN
                     ETKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTV
                     SGGTLWYLGGMIQANLEEIRKQNESLEKLNAKTWGVRYHEGSNGRFLVLPEGMKAETN
                     WTMENGKQNAVRLVQE"
     gene            2680..2913
                     /locus_tag="ACHUQ7_003839"
     CDS             2680..2913
                     /locus_tag="ACHUQ7_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HFR1137025.1"
                     /translation="MTELESHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVLHLSEQTERLSQLYSENWR"
     gene            complement(3459..3851)
                     /locus_tag="ACHUQ7_003840"
     CDS             complement(3459..3851)
                     /locus_tag="ACHUQ7_003840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1137026.1"
                     /translation="MALSACFRQQLNPVVNSLLKERIIHSPKELEHDNAVIRVLQKLQ
                     IQEVCNSTLWETAKQQLLQKSDGRYFAINSKHLAFPGPGESHAFCCIKYKNAIGINGN
                     NAETQSTHYQPYPYDKVSIWGPFPHNLT"
BASE COUNT          966 a          917 c         1207 g          952 t
ORIGIN      
        1 aaaaaaacca ccgctaccaa cggtggtttg tttgccggaa tctagagcta ccaactcttt
       61 cttccgaagg taactggctt ggtggagcac agataccaaa tgctgtcctt ctagtgtagc
      121 cgcagttagg ccaccacttc aagaactctt aatatctcaa tccaccttgt ccagttacca
      181 atggctgctg ccagtggcgt taagtcgtgt cttaccgggt tggactcaag acgatagtta
      241 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
      301 cgaacgacct acaccgaact gagataccaa cagcgtgagc tatgagaaag cgccacgctt
      361 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
      421 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
      481 ctctgacttg agcgtcggtt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
      541 gccatcggcg cggccttctt cctgttcctg gtcttttgct cacatgttct ttccggcgtt
      601 atcccctgat tctgtggata acagcatttc gtttttgagt gagtagatac cgctcgccgc
      661 agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag cggaaaagcg cctggacgtg
      721 cattttctcc ttacgcatct gtgcggcatt tcacacccgg tatggcgcac ttttcataca
      781 atccgcactg atgccgcatg gttaagccag tatacactcc gctatcgcta cgtgactggg
      841 tcatggctgc gccccgacac ccgctaacac ccgctgacaa gcccgtcaga ggttttcacc
      901 gtcatccccg aagcgagcga ggcaactgcg gtaaagctca tcggcgtggt cgtaaagcga
      961 ttcacagatg tctgcctgtt catccgcgtc cagttcgttg agcttctcca gcagcgttaa
     1021 tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga
     1081 tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgcgagag
     1141 gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gctgtgaggg
     1201 taaacagctg gcggtatgga tgcggcgggt ctgcctgggt gagccggttg cccgttccgg
     1261 gaggctgccg acactggcac cgccgttact gcgtcagctg gccgccatcg ggaataacct
     1321 gaatcaggca gcgaggaagg tgaacagcgg gcagtggtct tccggtgacc gggttcaggt
     1381 ggtggccgca ctgatggcca tcggggatga gctgcgccgg ctgcgtctgg ccgtcaggga
     1441 acaggggacg cgggatgata gttaagtttc atgccagggg aaaaggcggt ggcagtggtc
     1501 cggttgatta cctgctggga agggagcgta accgggaagg ggcaacggtg cttcagggta
     1561 atccggaaga agtccgggaa ctcatcgatg ccacgccatt tgcgaagaaa tacacgtccg
     1621 gtgttctctc gttcgcggag aaggagctgc cgccgggagg gcgtgaaaaa gtgatggcga
     1681 gctttgagcg tgtactgatg cccggtctcg aaaaaaatca gtacagcatt ctgtgggtgg
     1741 agcaccagga caaggggcgg cttgagctga attttgtcat tccgaacatg gagctacaga
     1801 ccggaaaacg cctccagccg tactacgacc gcgcagacag gccgagaatt gatgcctggc
     1861 agacgctggt gaatcaccat tacgggctgc atgacccgaa cgccccggag aaccgccgga
     1921 cgctgactct ccctgataac ctgccggaaa cgaaacaggc gcttgctgag agcattacac
     1981 ggtggataga cgctctttat catgtcgggg agataaaagg ccgtcaggat gtgattcagg
     2041 cgctcacaga ggcggggctt gaagtggtca gggtgacgcg aagcagtatc agcattgcag
     2101 acccgaacgg cgggaagaat atcagactga aaggagcatt ctatgagcaa tcttttacag
     2161 acgggcgcgg agttcgagaa aaagctgaaa gagagagccg aatctacaga gaaaatgctg
     2221 agcgacgagt tcaggaagct cgaagaatct gtaagcaagg ctgtgacatc aaacgagacg
     2281 aaaatcaaag acgctatagc cctgttcaca gcttcgacag aggaatcact gaaaaaacat
     2341 cgggaagggg tgaaagaggc gatgatgcag cacaggaagg acgtgttaaa gctggcaggg
     2401 aatacgggca tgatgttact ggggatggtc attttcctgt ttaccgtgag tggcgggacg
     2461 ctctggtatc ttggcgggat gatacaggcg aacctggaag aaatcaggaa gcagaacgag
     2521 agtctggaga agctgaacgc gaagacatgg ggcgtgcgtt atcacgaggg gagcaacgga
     2581 cgttttctgg tgttgcccga ggggatgaaa gccgaaacga actggacgat ggaaaacggg
     2641 aaacagaatg ccgtaaggct ggtacaggag tgacagagca tgacggagct ggaaagccat
     2701 ttgctgaacg cattagagca gctgcaacag gactatatgc agcggctgaa cgaatgggag
     2761 agcgccttcg tggaattgca gaaaatgttt tcgcttacgc aacgggacaa cgcgatgctg
     2821 aacgagcggg tcatgcagtt gagtcagcag gtgctgcact tgagcgagca gacagaacgc
     2881 ttgagccagt tatacagcga gaactggaga taagagagga acggttgata caggagcgcg
     2941 aacatgcgtt attcctggaa cgggaacgcc agccggaaat acaggaacgg acactggatg
     3001 gcccttcgct gggatggtaa tcagagacag gctaaaatgt tgtcagcgcg tttccggtgc
     3061 attttttggg ataatggttg gttcgggggc accgggaacg cgcttcagtc agaccttgct
     3121 gaaaacacag gaacgagtta aaacgcatac gggcgcgatt ctggtgtgtc ttttgtcgct
     3181 gtgttttgcc tctattgcca gatcgcgctc attcccctga acttctgttt tcccgctcag
     3241 gtccccgtct tcttcgcttc cgaaagcatg aataaaatcc gtttcagctg tctctggctg
     3301 gcaattcctg cgtgattcac atggctgcat agctatgcat gtggttaaaa tttaccgggt
     3361 gcgatcgcgg cagtttttcg ggtggtttgt tgccattttt acctgtctgc tgccgtgatc
     3421 gcgccgaacg cgtttcagcg gtgcgtacaa ttaagatatt acgttaaatt gtgagggaat
     3481 ggtccccata tactgacttt atcataggga tagggttggt agtgggtcga ttgggtttcg
     3541 gcattgtttc cgttaatgcc aattgcattt ttgtatttga tgcaacagaa ggcgtgagat
     3601 tctcctgggc ctggaaaggc taagtgttta gaattgatgg caaaatatct tccgtccgat
     3661 ttttgaagta gttgttgttt ggctgtctcc caaagggtgc tgttacaaac ttcttgaatt
     3721 tgtagttttt gcaggactct tattactgcg ttgtcatgtt ccaattcttt aggggagtgt
     3781 attattctct cttttaataa gctgtttacc acagggttta gttgttgtct aaagcacgca
     3841 gataatgcca ttgctacaca attttgttcc cgaggaagca tgtttggcgg ggtatgccgt
     3901 gtttgatgtg atatgtctat tctcatagaa ggtctctggc ttatagcatc aacaaatacg
     3961 gctataaaac aaaattttca tagagtgatt caagatcttc ttgagatcct tttttgcggc
     4021 gcgtaatctg ctgcctgcaa ac
//
LOCUS       DAZPCS010000235         4004 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZPCS010000235 DAZPCS010000000
VERSION     DAZPCS010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4004
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.240"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(139..3654)
                     /locus_tag="ACHUQ7_003841"
     CDS             complement(139..3654)
                     /locus_tag="ACHUQ7_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HFR1137027.1"
                     /translation="MDENALGFASYWRNSLADAESGKGSFKRKDAQNFTHWHGIAAGR
                     LDEAIVSKFFEGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTTFSINFDDSVDKTAETD
                     EEREARYAALQQEWRQYLYDSERLLKSVAGDWIEKPEQYELAEHGYIVKTAQSGGASS
                     HILSLYDHLLVCNKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGSGAMAGRWLPELKSFGAYFPSSSRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPGKECSSPEKVIELLHGQLAAKSEQLIRLNATWQTLSQ
                     IRAARELIANDIEQYLDNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRNKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLLNSAEREQTTYRQQIDSA
                     HEIVLKEQQAVQEWQRLAFDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMASIDDLQDEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCYDNIIGYCNTLCYHGKLLPKRGREESNLMPAMGYLHIDGKGELASSGSRYNLLE
                     AETIAVWLAENQQNIEAHYGKSLHEVVGIVTPFSAQVSTIKQVLGKQDISTGTNEKSL
                     TVGTVHSLQGAERAIVIFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPASSPRGLLAKYLFESEKNALSFDYKERKDLKTAGTKIYTLHGVEQHDNFLNQTF
                     ENTSKHITIISPWLTWQRLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHNDFEKRKE
                     KQQNFKAALEKLNALGIATKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLERRQV"
BASE COUNT         1030 a         1016 c          834 g         1124 t
ORIGIN      
        1 gagcctctga ttcttccgga cagaagataa tcatgtcggt atgaatgtta taattaagga
       61 ctgtgcaata atagccattc cattcagcca tcttacgctc gaaaacgatc ggtaagctgg
      121 ctactcacca ttcctgctct aaacctgacg cctctcaaga ctattataaa tagcctcaat
      181 ctcacccttc aggttatcac cgcaataaac cattgatgta tcgtatcgtt catatcgcgc
      241 ttcccgtgtc gcactaaacc agttgaacga tcccacacac agcaaaccat catcaccaat
      301 aacaattttg ctatgaacac ggtttaccag ctttgtagca atacccagcg cattcagttt
      361 ctccagcgcc gctttaaagt tctgctgctt ctcttttcgc ttctcaaaat cattatgttc
      421 agtgttgtag cttctgtcag tgactatcgt gacgttaatt ccacgtgaac acgccgcaat
      481 catggaatca agaaaaccgg tttgctccag cctttgccag gtcagccatg gagaaattat
      541 cgtgatgtgt ttactggtat tttcaaatgt ctgattcagg aaattatcat gttgctccac
      601 accatgaagt gtgtagattt tggtcccggc ggtttttaaa tccttacgct ctttataatc
      661 aaaagagagc gcattcttct ctgactcaaa gaggtatttt gccagtaatc cccgtggcga
      721 tgaggctggc tggacctcaa acaggtccat atcgccgaag accagaaaac tgtccttcgc
      781 acgggagact gcaacattca gcatgctgtt atcgctatca ataaacccgc cgtcttcatg
      841 ttttgaatag actggcgaga atatcacaat cgctctttcc gctccctgaa gagagtgcac
      901 ggtgcccact gtgagcgact tttcattcgt gcctgtactg atatcttgtt tgcccagcac
      961 ctgtttgata gtggatacct gagcgctaaa aggcgtcaca ataccgacaa cttcatgaag
     1021 cgatttaccg taatgcgctt caatattttg ctggttctct gccaaccaga ccgctatcgt
     1081 ttcagcctca agcaaattat atcgacttcc actacttgcc agctctcctt taccatcaat
     1141 atggagatac cccattgcgg gcattaaatt gctctcttca cgccctcttt taggcaacaa
     1201 cttaccgtga tagcagagcg tattacagta tccaataata ttgtcgtagc accggcggtg
     1261 ttcatatagg tacataccac gagccagttc gggatcatat tgatagcgcg aagcaaactg
     1321 cgctattttc ataacgctgc cagatgcggc acttttacca aggtctgcga ttgccgtata
     1381 tttctcggta atctcttctt gcgtactgcc agacagaatt ttttccgcca gcatgttacc
     1441 gacatcaatc gcaggagcaa tactccatat tggcgggatc tgctccgtat cgccaatcac
     1501 taatgccttc ttagctaatg caaacgaggc agcagccact tcaggaagca cctgcccggc
     1561 ttcatcgaca atgagtaaat cggcaaaatc atacaaataa cttttctcga atttacgttg
     1621 tcctttgtgc tcacttatct gcatattacc gggcagcata tagcatgtca tcaccacaca
     1681 tggcgtgagt ttcattcgac gttgccaacg ggcggttacc cctttagcac cttttttctt
     1741 cttctcatcc tgcagatcat caatgctggc catatccatc agccaacgac cttcccagta
     1801 gtgagtcgtc agtaaaaatg cagggaagcg aatctgcgta tcagccagtt catcggcctg
     1861 tgagaagctc agttcctcgt cgccctcata ccctaaatca aatgccagcc tctgccactc
     1921 ctgaaccgcc tgctgttctt taagaacgat ttcatgggcg gagtcaatct gctgccggta
     1981 tgttgtttgc tcgcgctcag cggaattgag cagcccatca atattacgtt cgatagtttc
     2041 aggatcagac cactgatttc ctgcaatcag cgcgcctaat ttatcttcga gaaacagctg
     2101 tatttggtac tgtcgcttat tgcgaaccgc cgggagccag gaaaataatg aatagatcag
     2161 tgattcaccg gcgcgatatt ttttccattc cgttttagca ctcttcagta gagtgacttt
     2221 ttgttcttgt ccggaaagta atttatttaa attatcgaga tattgctcaa tatcattagc
     2281 aataagctca cgcgcagccc gaatctggct taacgtttgc catgttgcgt tcagtcttat
     2341 cagttgctca gattttgctg ccaactgacc atgcaagagt tcaatgacct tttcagggga
     2401 tgaacactct tttccaggaa aggctgcctt agccttttcc agataaaaca gcagtgcatc
     2461 ctctacatac tcttttgact caacctggtt gaagaaatct tcagtttgat attttttggc
     2521 tgcctcagct ttacgactgc ttgagggaaa ataagcaccg aagcttttca gctctggcaa
     2581 ccatcgcccg gccatcgcac ctgaaccttg cgaaaagtct ttcccgaatg cctcaataat
     2641 gttcgttaca gcctggttat tcgttgaagt cgcgataata accggaggct cagatttttc
     2701 gagagccgct ctggcccact gcgtggcgat gatagaaagc accagcgtgg tttttccggt
     2761 tcccggaggg ccattaacag caaggatatc gccatgtctt gcatccagaa aatggcttaa
     2821 ggcatcgcgc tgagcctttg ccagcggaaa cttatctccg gagtgtccaa gcctgtcgct
     2881 gaattttgct cctggggcca gcaaagactc tgcagcatga acctctcgcg aggcgaagcg
     2941 attgaagagc ggcacatcct tattgcaaac aagcaggtga tcataaagag aaaggatatg
     3001 ggaactggca ccgccagatt gagccgtttt aacaatataa ccgtgctcag cgagttcata
     3061 ttgctcaggt ttttcaatcc agtcgccggc aacgctcttc agtagcctct ctgagtcata
     3121 cagatattga cgccactcct gctgcaaggc ggcatatcgt gcttcccgtt cttcatccgt
     3181 ttcggcagtc ttatcaacgc tgtcatcaaa gttgatagag aacgtggtat gggtcgtctt
     3241 gtatttgtca tactgcccaa tctcaccaat cgaaaatgct ccttttggca aaggttcaag
     3301 caggtctctg ggaatggagg tcgctggcgt cggatataaa aaaccctcac ggcttagcaa
     3361 ggctggcgtc actatcgggg taacaatatc aggcgcgcct gcggaacggt ccttaccatg
     3421 ctgcagtaac cggaaataaa cttttgggcg caagatgaca tcgaccgttt cgacatcgtc
     3481 tttttctccc tcaaaaaatt tactgacaat cgcttcgtca agacgtcccg ccgctatccc
     3541 atgccagtga gtgaaatttt gggcgtcttt ccgtttaaaa ctgccctttc ctgactcagc
     3601 atccgcaagc gagttgcgcc agtatgaggc aaaccctaaa gcattttcat ccattaccag
     3661 tcctgtgact caatgaaatc cattgattta tcgaatataa aatcaccgta tggacagcta
     3721 ttgtccatat tgaaaaagtt ggatgcaatg ttttgtttat aattcgatat caaatgaact
     3781 ggttgtacaa ggttcagaac acaagctcat taaacagtac gtcaggacta ttgatttcat
     3841 aacagtgaat acatttttta ccgtttataa taactacctg tgttggacgg ggataccaga
     3901 gcagccgaaa acaccggggg ttattacctg ctctcatgga aaccatatat tcctggtgct
     3961 aatcacagtc ataaatagcc tgaagaacgt gacgaaggga cttt
//
LOCUS       DAZPCS010000236         3979 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZPCS010000236 DAZPCS010000000
VERSION     DAZPCS010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3979
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.241"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            63..757
                     /locus_tag="ACHUQ7_003842"
     CDS             join(63..309,309..757)
                     /locus_tag="ACHUQ7_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR1137028.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
                     MLTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHE
                     KVIGAFIEKHMFY"
     gene            complement(876..1274)
                     /locus_tag="ACHUQ7_003843"
     CDS             complement(876..1274)
                     /locus_tag="ACHUQ7_003843"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1137029.1"
                     /translation="MNSTINVFGEPSKNLAMLIEEGNLNAPAILNDIKKHVNHLISLH
                     PVNEIILGCTHYPIVQNFFEAVAPDIKFINPAHDQAISIKNHLGQLNLLNSSNIGTLQ
                     INTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(1271..1684)
                     /locus_tag="ACHUQ7_003844"
     CDS             complement(1271..1684)
                     /locus_tag="ACHUQ7_003844"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HFR1137030.1"
                     /translation="MFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNMNAPY
                     GNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEEYSGYAKSFPFPILSII
                     EPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKN"
     gene            complement(1718..3178)
                     /gene="gadC"
                     /locus_tag="ACHUQ7_003845"
     CDS             complement(1718..3178)
                     /gene="gadC"
                     /locus_tag="ACHUQ7_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glutamine/gamma-aminobutyrate
                     antiporter GadC"
                     /protein_id="HFR1137031.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            complement(3285..3915)
                     /locus_tag="ACHUQ7_003846"
                     /pseudo
     CDS             complement(3285..3915)
                     /locus_tag="ACHUQ7_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006816255.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
BASE COUNT         1269 a          833 c          761 g         1116 t
ORIGIN      
        1 ggatgggtga tgcctctaat tagttgaatc tgatgtataa tacgggcttt tgaggttatc
       61 tcatgaccag cgttaatatt cattgtcccc gttgtcagtc agctcaggtt taccgccatg
      121 gtcagaaccc taaaggccgt gacagacttc gctgccgtga ctgccaccgt gtgtttcagt
      181 tcacttatac ttatcaagca cgtaagccgg gtatgaaaga gctgataact gaaatggcct
      241 ttaatggtgc cggggttcgc gataccgcca ggacactgaa aattggtagt aacaccgtca
      301 tccggacttt aaaaaactcg cgccaaagcg aataacttct tcgcctgttg cccatgctga
      361 tgtggcgttt atctgcgagc ttgatgagca atggagctac gttggcagta aagcccggca
      421 acattggctc gggtacgcgt acaacaccaa aacaggcggt gtactggcct acacttttgg
      481 tccccgaacc gatcaaacgt gccgggagct actggcactg cttacaccct tcaacatcgg
      541 catgctgacc agcgatgact ggggcagcta tggccgggag gtgccgaaga ataagcatct
      601 gaccggcaaa atattcaccc aacgcattga gcgtaataat ctgacgctac gcacccgcat
      661 taagcggttg ggtcgtaaaa caatctgctt ctcacgctca gttgagatcc acgaaaaagt
      721 gatcggagcg tttatcgaaa aacacatgtt ctactaattg gatgcactac ccagtatgta
      781 actgggtttg tccataaaat gcaccttact cagttggtgt ccaacttttg gggtgcagtt
      841 cagaaggacg ggattttacg gtgtaccgga taaatttaaa actgcctgat actgaatgta
      901 tgaggtttcg taatgctcaa ttcccctagt accgttttgt agatctccat acttccacta
      961 gtatttattt gcaaagttcc tatatttgag gagtttaata aatttagctg tcccaagtga
     1021 tttttaatag agatagcctg atcatgtgca ggattaataa attttatatc cggtgcaact
     1081 gcttcaaaaa aattctgaac aattggatag tgagtacagc cgagaataat ttcatttact
     1141 ggatggaggg aaattaaatg gttaacatgt tttttgatgt cattcaagat tgcaggagca
     1201 ttaagattac cttcctcaat taacattgca agatttttgc ttggttctcc aaatacattt
     1261 attgttgaat tcaatttttt attagctctt tgtgacaccc cgttttaata gtaaactcgg
     1321 tagcgataat accaacattt ttatattgct ggcgaatcac atcttctact gcaggttcaa
     1381 tgatggataa gataggaaac ggaaagctct tagcatatcc gctgtattct tcggattcca
     1441 aagttgagga tatagtatta catgcgacag caactagttt gacattcctt gactgtaaaa
     1501 aagtcagcat ttttttagtc aaactgatga tatcatcggc agaacggttg ccataaggtg
     1561 cattcatatt atctccacaa tagataatat cttcaccagg aagcaaatta attatttcct
     1621 ttaccgatgt caatccaccg acacctgagt ctattacacc aattggagat tcagcactaa
     1681 acatatttaa cctcatcttt tgtagcttat ggaaaattta attactagtt cttttttccc
     1741 aagaaaatgg ttcgaaatca gagtttggat ctttccagtc agattttcgg aaggcgtaaa
     1801 ttaatattgg aattaataaa aatagggcaa ctaatactaa caaataaaga acatagctgg
     1861 ttggactacc agttgggatt tgctcgggcg gaataaaact taatacaaaa gccagaaaag
     1921 accctaagaa tccaagtgtt gcccagaaaa ataaagcagg ggctcgatat gggcgttttg
     1981 tatcaggttg agaatgtcgt agatagatag cactagcgaa catgagcata tacataacta
     2041 aatagagcat aactgttaac tgtgataaaa tctgaaaagc tgcttgaact gaaggcatta
     2101 tcacaaaagt aacagaaaga actgagacaa ttataccttg tacaaacatt agatggtgcc
     2161 ccattccatg acgattggtt ttttggaaaa acttaggtaa ataaccagct ttcgcgatcg
     2221 ctaatacacc tgtattaggc cctgcaatcc aagtaaccac cccacccagt acgccaattg
     2281 ctagcataaa tgccatcaca gagccaagcc atggcactcc agcccattcg aacagcatat
     2341 cgtaggcaac caacaaactt tgagttagac taattttatc tgtaggaata ataaaggcga
     2401 cccctaaagt actgagcagg aatattgcaa tagtacctat agaagcgatg gtaattgcaa
     2461 ttgggtattt tttagttgga ttatctaaat cgttaacatg gaccgcattc atttccatcc
     2521 ctgcataagc aagaaatatg ctggcggcaa gaacaatatt ctggaagtta gctatattag
     2581 ggaaaagatc accccaacct aatgggatca ccgctggttt accgctgaac cacagatagg
     2641 agaaaccaag aataacaagg ataatagctg gaataattgt ccctataata ccgcaccact
     2701 tagatacctg cgagaaaata ccaacgccac gtaatgcaac acaaatagct ccccagtaaa
     2761 caaatagtac gaaaaataaa atataccatt tattactggc aattgattct gccaccccca
     2821 tatcaacatc aataaatgca accgagactg caccaaatgt cagtactgtt ggaaagtagg
     2881 ggatgacttg tagccaggtc ataagcattc ctacaaaacc ccagcgagcc ccaaatgctt
     2941 ctcctatcca tcgaaatgcc cccccttttt gtggatatgt tgtagctagc tcagccgcaa
     3001 caaaggcaac aggtactaaa aacataattg cagcaaaggt atagtaaaaa atggcaccta
     3061 agccgtattc tgcttcagaa ggtataaatc gtagacttac tacagcagtt atattcatga
     3121 tagcgagtgt ccccactgtc attttcttac ttttgggtgc gatattagca ttagacattt
     3181 ttatactctc gaacattttt aaaatagaaa catataatta cgatgtaaat ataaaaacta
     3241 atattaactt taacacttat cttaaaaaca ctaacgcaat ctgctttaca aaaataagta
     3301 atacctaaag tcgtaacgac accaatagtg ttttcatcga catgcttcaa aacctcttta
     3361 gcattcatta tcaggtgatg atcatcaagt ggaatttcac acaatttcac atcacagtaa
     3421 cggcagaatt tatgccagca tatttgcaca ggatcgcaaa tcaggttcgg ttgatttacc
     3481 ttgtacttcg cgttttttac accactgcca ttttaacgcc aagcggccga gcatagcagc
     3541 ttcagatgaa ccaatggttg agcaacctaa ggtgtgtcgc gaatcgggga aactccacga
     3601 atcggaaagc atacgcacac agcgaccttc aggttccgcc atttgtggat attcatcttt
     3661 ataaatcatg tttttatcaa tagctaaatt aagcaactca cggatttcat catcaagcca
     3721 tgtttgacag aaagtagcta agttttgtcg tgaatcccca tcaaggatga gctcatcacg
     3781 tatggcgctg aatacatttc gagatttctt ctcatctaca cgcaaatgat atttatgcaa
     3841 tataaatgaa agctcaagtg agctatgaat atcgactgct tctctataac aagaattacc
     3901 tgatttattt ttcataataa acgccactca atacaatcag attaaacatt acaaacacaa
     3961 tctggatttt gcaatttta
//
LOCUS       DAZPCS010000237         3976 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZPCS010000237 DAZPCS010000000
VERSION     DAZPCS010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3976
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.242"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..224
                     /locus_tag="ACHUQ7_003847"
     CDS             <1..224
                     /locus_tag="ACHUQ7_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFR1137032.1"
                     /translation="FQRDRYGRILGQVYAPDGMNINQFMVRAGAAWVYEQYNTDPVLP
                     VLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            270..479
                     /gene="hha"
                     /locus_tag="ACHUQ7_003848"
     CDS             270..479
                     /gene="hha"
                     /locus_tag="ACHUQ7_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFR1137033.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            518..1108
                     /locus_tag="ACHUQ7_003849"
     CDS             518..1108
                     /locus_tag="ACHUQ7_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFR1137034.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            1348..1608
                     /locus_tag="ACHUQ7_003850"
     CDS             1348..1608
                     /locus_tag="ACHUQ7_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFR1137035.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            1832..1906
                     /gene="tap"
                     /locus_tag="ACHUQ7_003851"
     CDS             1832..1906
                     /gene="tap"
                     /locus_tag="ACHUQ7_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFR1137036.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1899..2756
                     /gene="repA"
                     /locus_tag="ACHUQ7_003852"
     CDS             1899..2756
                     /gene="repA"
                     /locus_tag="ACHUQ7_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFR1137037.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            3457..3648
                     /locus_tag="ACHUQ7_003853"
     CDS             3457..3648
                     /locus_tag="ACHUQ7_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1137038.1"
                     /translation="MAENGYGLAGLGMGKVKSVNQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGIRIIQAAFNAFAKV"
     gene            complement(3878..>3976)
                     /locus_tag="ACHUQ7_003854"
     CDS             complement(3878..>3976)
                     /locus_tag="ACHUQ7_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1137039.1"
                     /translation="GSQMRFTGLKRGYRLAEQVLMHEPLARMRWCG"
BASE COUNT         1074 a          988 c          988 g          926 t
ORIGIN      
        1 attttcagcg agatcgttat ggtcgtattc tcgggcaggt ttatgcacca gacggaatga
       61 atattaatca gtttatggtc cgggccgggg ctgcctgggt atatgagcag tacaacacag
      121 atcccgtgct acctgtcctt cagaacgaag ccagacaaca aaaacgtggg ctctggtcag
      181 atgctgatcc ggttcccccc tggatatgga ggcaccgtaa ataaagcgat ggcaatccta
      241 tcattcatct gttctcagcg ggaataaaaa tggcaaaaac aaaacaggaa tggctgtatc
      301 agttacggcg atgttcatca gtcaatacgc tggaaaaaat tattcataaa aacagagaca
      361 gtttatcaaa cagtgaacgt gaatcattta attcagcagc agaccaccgg ctagcggaat
      421 taatcaccgg aaagttatat gacaggatac caaaagagat atggaaatat gtaaggtaat
      481 aattcagcaa taattaattg ccaggagcag ataaataatg aagttgatta tttttattct
      541 cattgttctg attattgcag cgctacttat ccggattatc ctcagaagtg taaatcagca
      601 ttctcctttg ctgatgcaat tgcatgcggc aggtatacgg actggtgatg cagagcgaat
      661 actgtccagc ggtgaatact ggcaacgtca gaagacgctg ctgacagaaa gggaagtcag
      721 ttttatgaaa ggactgttca gaattgtgga tatgaagcgg tggtatctgt gtccgcaggt
      781 acgggtagcg gatatcgtcc agctgaacgg gaatatccgg ccacgatcac gccagtggtg
      841 gcagttattc aggatggtgt ctcagtggca tgttgatgtg gttatcgttg agctgcgttc
      901 gttcagtatt gttgcagcag tagagctgga tgatgccagc catttacgac cggaacgcag
      961 acgccgggat attcttctgg aagaggttct gaggcaggct ggtattccgt tgctcagaag
     1021 ccacgatgcc agaaaactgc tgcagatgac cggagaatgg ctgaatacaa caggggctga
     1081 tcagcagtca ccggaacatc gtagctgacg ccttcgcgtt gctcagttgt ccaaccccgg
     1141 aaacgggaaa aagcaagttt tcctcgctcc cggcgtttcc ataactgaaa accatactat
     1201 ttcacagttt aaatcacatt aaacgacagt aatccccgtt gatttgtgcg ccaacacaga
     1261 tcttcgccac aattctcaag tcgctgattt caaaaaactg tagtatcctc tgcgaaacga
     1321 tccctgtttg agtattgagg aggcgagatg tcgcagacag aaaatgcagt gacttcctca
     1381 ttgagtcaaa agcggtttgt gcgcagaggt aagcctatga ctgactctga gaaacaaatg
     1441 gccgctgttg caagaaaacg tcttacacac aaagagataa aagtttttgt caaaaatcct
     1501 ctgaaagatc tcatggttga gtactgcgag agagagggga taacacaggc tcagttcgtt
     1561 gagaaaatca tcaaagatga actgcagaga ctggatatac taaagtaaag actttacttt
     1621 gtggcgtagc atgctagatt actgatcgtt taaggaattt tatggctggc cacgccataa
     1681 ggtggcaggg aactggttct gatgtggatg tacaggagcc agaaaagcaa aaaccccgat
     1741 aatcttcttt aactttggcg agtcagaaag attaccgggg cccacttaaa ccgtatagcc
     1801 aacaatcaag ctatgcgggg agtatagtta tatgcccgga aaagttcaag acttctttct
     1861 gtgctcgctc cttctgcgca ttgtaagtgc aggatggtgt gactgatctt caacaaactt
     1921 attaccgcca ggtaaagaac ccgaatccgg tgttcacacc ccgtgaaggt gcccggacgc
     1981 ttccgttctg cgggaaactg atggaaaagg cggtgggctt cacctcccgt tttgatttcg
     2041 ccattcatgt ggcacatgcc cgttcgcttg gtctgcgtcg gcgcatgcca ccggtactac
     2101 gtcgccgggc cattgatgcg ctgctgcagg ggctgtgttt tcactatgac ccactggcca
     2161 accgcgtcca gtgttccatc accacgctgg ccattgagtg cggactggcg acggagtctg
     2221 ctgccggaaa actctccatc acccgggcca cccgggccct gacgttcctt gcagagctgg
     2281 gactgattac ctaccagacg gaatatgacc cgcttatcgg gtgctacatt ccgaccgata
     2341 tcacgttcac atctgcactg tttgctgccc tcgatgtatc agaggaggca gtggccgccg
     2401 cgcgccgcag tcgtgtggaa tgggaaaaca gacagcgcaa aaagcagggg ctggataccc
     2461 tgggtatgga tgaactgatg gcgaaagcct ggcgttttgt tcgtgagcgt ttccgcagtt
     2521 accagacaga gcttaagtcc cgtggaatga agcgtgcccg tgcgcgtcgt gatgcggaca
     2581 ggcagcgtca ggatattgtc accctggtga aacggcaact gacgcgtgaa atctcggaag
     2641 ggcgcttcac tgccagtcgt gaggcggtaa aacgtgaagt ggagcgtcgt gtgaaggagc
     2701 gcatgattct gtcacgtaac cgtaattaca gccggctggc cacagcttcc ccctgaaagt
     2761 gacctcctca gaataatccg gcacgcgccg gaggcattcg cacgcctgaa gcccgccagt
     2821 gcacaaaaaa tcagcgctac atacaaaaaa caacctcatc atccaccttc aggtgcatcc
     2881 ggtcccccct gtttttgata caaaacacgc ctcacagacg gggaattttg cttatccaca
     2941 ttaaactgca agggacttca ccataaggtt acaaccgttc acgtcataaa gcgccagacg
     3001 tcaaccctac agggtgcaat gtatctttta aacacctgtt tatatctcct ttaaactact
     3061 taattacatt catttaaaaa gaaaacctgt tcactgcctg tcctgtggac agacaaatat
     3121 gcacctccca ccgcaagcgg cgggccccca accgaagccg gtttagttac aacatgcaaa
     3181 cataaacccc taaaaaaccc aagtccaacg caaaactgaa accacaacgc ccccctcata
     3241 actgaaaagc gccgccgccc ccgcccaaag ggcgggaaca gagtcgcttt taattatgaa
     3301 tgttgtaact acatcatcat cgctgtcagt cttctggctg gacgtatcga atacacgctc
     3361 gtaagcggcc ctcacggccc gctaacgcgg agatacgccc cgacttcggg taaaaccctc
     3421 gtcgggacca ctccgaccgc gcacagaagc tctatcatgg ctgaaaacgg gtatggcttg
     3481 gcagggctgg ggatgggtaa ggtgaaatct gtcaatcagt accggctgac gccgggcttc
     3541 ggcggtttta ctccggtatc acatgtaacc acagcgtgcc gccttccatg ccgctggcgc
     3601 ggcatcagaa taatacaagc agcatttaat gcatttgcaa aagtgtgagt aatattaagt
     3661 ggtcgtttca gggatatcgt taagacttga gcgataggaa ttaaaacccc aaaaagatta
     3721 aaaaaacatc acaaaacgga tgtttcttca acaccacttt tgctccatat gaacggaacc
     3781 gacgattaaa ctggatggct ctgattgatt cagggtatga atggcggttt tttgctccgt
     3841 ttccctcaaa atggacgcaa cttcccctct gcggctctca gccgcaccac cgcatccggg
     3901 ccagcggctc atgcatcagg acctgctctg ccagacggta gccccgcttc agccccgtaa
     3961 aacgcatctg actccc
//
LOCUS       DAZPCS010000238         3885 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZPCS010000238 DAZPCS010000000
VERSION     DAZPCS010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3885
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.243"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            106..1164
                     /gene="tsuA"
                     /locus_tag="ACHUQ7_003855"
     CDS             106..1164
                     /gene="tsuA"
                     /locus_tag="ACHUQ7_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HFR1137040.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISAQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVTVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGVTSPTANILQFLVAGDVKYINWGVFLVLGIFVEAFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            1178..1405
                     /gene="tsuB"
                     /locus_tag="ACHUQ7_003856"
     CDS             1178..1405
                     /gene="tsuB"
                     /locus_tag="ACHUQ7_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HFR1137041.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(1448..2872)
                     /gene="sbcB"
                     /locus_tag="ACHUQ7_003857"
     CDS             complement(1448..2872)
                     /gene="sbcB"
                     /locus_tag="ACHUQ7_003857"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFR1137042.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDID
                     TLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRHQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            3054..>3885
                     /locus_tag="ACHUQ7_003858"
     CDS             3054..>3885
                     /locus_tag="ACHUQ7_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1137043.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFDNYVSTTLREKTQALYRSTVMKRMHDAFPNRPASSITVKQWVDLLTEEEKDNPRR
                     ARQVLSQLRSAISWCMRRQLIDSCAIMSIQPRDFGSRAEVGDRVLSYHELAKIWLAIE
                     RSRASTSNKLLHQMLMLWGARLSELRLATKTEFDLLDNVWTVPKEHSKMGNVIRRPIF
                     E"
BASE COUNT          929 a          909 c         1005 g         1042 t
ORIGIN      
        1 cctcattaat aaccatttgt tctaatttag agaatttggt tattggtctg cattccacca
       61 gtcatgaaaa atttccccat taacattcat ctggttaatt aatttatgtt ttcaatgata
      121 ttaagcgggc taatttgtgg tgctctgctg ggatttgtca tgcagcgtgg gcgtttttgc
      181 ctgactggtg gttttcgcga tatgtatatc gcgaaaaata atcgcatgtt ttacgccttg
      241 ctgattgcaa tttcggcaca aagcgtgggg gtttttgcgt taattcaggc gggcctactg
      301 acttacgaag ccggggcgtt cccgtggctt ggtactgtta taggtgggta tcttttcggg
      361 ctgggaattg ttctggcggg tggatgtgcc accgggacct ggtatcgcgc gggtgaggga
      421 ttgatcggca gttggatcgc gcttttcact tatatggtga tgagtgcggt gatgcgttct
      481 ccacacgcca gtggtttaaa tcaaaccttg cagcactaca ctactgaaca taactctatt
      541 gctgagactt ttaatttgtc tgtgtggccg ttggttaccg ttttgctggt gataacgctc
      601 tgggtggtga tgaaagagtt gaagaagcca aaactcaaag tcgcgacctt accgccgcgc
      661 cgaaccggga tcgctcatat tctgtttgaa aaacgctggc atccctttgt cactgctgta
      721 ctcatcggtt tgattgcgct tttagcctgg cccctgagtg aagcaaccgg acgcatgttt
      781 gggttaggag tcacttcccc aacggccaat attcttcaat ttctggtcgc gggtgacgtg
      841 aaatacatta actggggcgt tttcctggtg ttagggatct tcgtggaggc atttattgca
      901 gccaaagcga gccgtgagtt ccgcgttcgc gcagctgatg cacaaacaac attacgtagc
      961 gggttaggtg gtgtactgat gggcttcggt gccagtattg caggtggttg ctctatcggt
     1021 aatggactgg ttatgactgc aatgatgacc tggcagggct ggattggcct tgtatttatg
     1081 attctcggag tctggactgc gtcctggctt gtgtatgttc gaccgcagcg taaggcgcga
     1141 ctggcaactg ctgcggcaaa ttaagtaagg tgtaaagatg gcgattaaaa agctggatgt
     1201 ggtgacgcag gtctgtccgt ttccactcat agaagcaaaa gcagcactgg cagaaatggc
     1261 cagcggcgat gagttggtaa ttgagtttga ttgcacccag gcaactgaag caatcccaca
     1321 gtgggcggca gaagaaggac atgccatcac cgattatcag cagattggcg atgcagcgtg
     1381 gagcatcacc gttcaaaaag cctgactcaa cattgtcctc cgccgtacca gcggcggcgg
     1441 cttcaaatta gacgatctct tccgcgtact gccaaagtgc ttttaacagc gccactttct
     1501 ctttgtcatc ggcatattgt tgtaccagca tctgcaattc atcagcataa ccctgcaaaa
     1561 actctggcgt gaagacctgg tggcggtgct ccagccagcg ttgctgctcg gcataatcca
     1621 gcgtccccgg gaagttgcgt gcccgataat tgaacaacaa cttttcaatt cgtttatcaa
     1681 caaaagtgat atccagtgcc ggtaaattac gcggctcggt ttccagcaca attttcattg
     1741 ctgcacgatc ggcgtcactg aaaaagccat tataaagctg tgcatccaca ttatctgaag
     1801 gtgtaaacgg ttcggcttcc gcgaatatcg ctaccacttt ttcacgcact tgcggatttt
     1861 cacgcagaat ttttaggtta tcgaggcaat gctgacgatt aattcccagt cggtcggcat
     1921 cttccgggcg tagcgtattc gcctgggcca gtaccggaca tttattgata tgcaccagct
     1981 taaccggaac ggcggcgtta tcgccaagat cggcttttgc ggtatataaa cgctcgcgca
     2041 atgtgtcgat atccagctcc agtaatggcg aaatgtctcc tgccaaatcc gccataatta
     2101 cggcattgcg attttcagga tgccacgcca gcggtgccac ccagctggta ttgccacgcc
     2161 atgccccaaa cattccggaa acgtgagcca ggggtttcat ctgcggaaca tcaatcaacg
     2221 ccatcagttt gtgtttatta cgatgggtaa agagataatc aaacaggcgt ggctgacgcg
     2281 tttttaccag tttcgccatc gcaatagtgg cgtacacatc agccatcgca tcgtgggcgt
     2341 tgctatgttc aataccattc gctttggtta aatgctcaag gcgaaagctc ggtagaccgt
     2401 catcattttc aggccagttt attccttccg ggcgcagggc ataacaggca cgcataacat
     2461 ccagtaaatc ccagcgcgag ttatcatgct gccagctcca ggcgtaagga tcgtagaaat
     2521 tacgataaaa aacgttgcgt gtgacttcgt cgtcgaaacg cacattgttg tagcccagaa
     2581 tacaggtctt cggtacggta aaaagcgagt gaatacgggc ggcaaacgcg gcttcgtttt
     2641 ctcctttcgc ccgtgcctcc tgcggggtaa taccggtaat taatactgct ccaggccggg
     2701 gtaaatagtc atccgcgggc ttgcagtaaa agacttcggg ttcgccgatg acattgaatt
     2761 cgtcatcggt gcgaatggct gcgaactgtg cagggcgatc taacgcgggg tgcgtgccaa
     2821 aggtttcgta atcgtgaaag aggaaggtag gttgtttatc tgtgtctgtc attgtctagc
     2881 attgtccact gtagttaatt aactgtctgt tataactcga aaagcaaccc ttttttcacc
     2941 tgagtatgtc tacgaacgtc aaatatagtt gtttactgtg cgctgttgtc catgtgatat
     3001 tgtgtaccag atgtgtgcca aactattatt tctgagcgta ccaaatatta tttatggcta
     3061 taagcgacac aaaactgcgt actatttatg gtaaaccata ttcgggccca caagaagtgg
     3121 ctgatgccga tggcctcagc gtacgaattt caccgaaggg ggtcatccag ttccagtacc
     3181 gctatcgctg gcatggcaag cctaatcgac ttgggcttgg tcgataccca tccctgtctt
     3241 tgaaggatgc cagacagatc actgctgact tgcgaaagct ctatttctca ggaacggatc
     3301 cacgcaccta ttttgaagag aaggtggaga actccatgac ggtcgcccag tgtctcgact
     3361 actggttcga caactacgtc tctacaactc tcagagaaaa gacccaggca ctttatcgat
     3421 caacggttat gaagcgcatg catgacgcct tccctaatcg tccggcatct tctatcacgg
     3481 ttaagcaatg ggttgacctg cttaccgaag aagaaaaaga taatccacgc cgagcaaggc
     3541 aggtgctaag tcaactaaga tcagcaatta gttggtgcat gcggcgtcag ttgatagata
     3601 gttgcgcaat tatgagcatc cagccaaggg acttcggctc ccgcgctgag gtaggggatc
     3661 gggtactgtc gtatcacgaa ctggctaaga tttggcttgc tattgaaaga agccgtgcgt
     3721 ctacgtcaaa taagctcctt catcagatgc ttatgctgtg gggggcgagg ctctcagagc
     3781 ttaggctggc aacaaagaca gaatttgacc tgctggacaa cgtatggacc gtaccgaaag
     3841 agcatagcaa gatgggtaat gttatccgcc gtccaatctt cgaac
//
LOCUS       DAZPCS010000239         3850 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZPCS010000239 DAZPCS010000000
VERSION     DAZPCS010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3850
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.244"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(131..1480)
                     /gene="lysC"
                     /locus_tag="ACHUQ7_003859"
     CDS             complement(131..1480)
                     /gene="lysC"
                     /locus_tag="ACHUQ7_003859"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFR1137044.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     regulatory      complement(1572..1768)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            2005..3654
                     /gene="pgi"
                     /locus_tag="ACHUQ7_003860"
     CDS             2005..3654
                     /gene="pgi"
                     /locus_tag="ACHUQ7_003860"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFR1137045.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHISEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
BASE COUNT          973 a         1030 c          970 g          877 t
ORIGIN      
        1 ttttctgctc attattagct ccttctgatg ccattctatt tcaggaagga gtgtccgtta
       61 aactcaggct acctcatagt gacaagaaaa tcaatacggc ccgaaatata gcttccaggc
      121 catacagtat ttactcaaac aaattactat gcagtttttg caccacctgc tcggcatctt
      181 cgccgggcac caggaagcac aggttatggc tggatgcgcc gtaacaaatc atgcgaatgt
      241 tgaacggttc cagtacgccg aatacctctt tgccaacgcc gcaggctttt gacaggtcat
      301 tgccaatcaa cgcgaccagc gccagacctt cttccacctc cacccggcac agtgcggaaa
      361 gctccatcag cagagattgc gtcagcaacg tatcgccagt ggaggttgaa ccggtggtat
      421 caagggttaa tgccacgctc acttctgacg tggtgattaa gtctaccgaa atattatgcc
      481 gcgcgaggat gccgaaaact tccgcgagga agccgcgaga atgcagcata ttcaggctgt
      541 gcaaagtgag cagagtctga ttgcgacgaa gcgccagcgc gcggaacagc ggcggatttt
      601 cagttttatt gcacaccagc gtaccacctg cgcgtgggtc tttgctggag ccgacaaaga
      661 ccgggatatc gctgcgtact gcgggtagca acgttgccgg atgcagtact tttgcaccaa
      721 aagttgccat ctctgccgct tcggcaaacg cgatttcatc aatgcgtttt gctgcggaaa
      781 ctacgcgtgg atcggtggtg tagatgcccg ggacgtcggt ccagatatca acacgagatg
      841 cgtgtaaagc ctccgccagc aaggctgccg tataatcgct gcctccacgg ccaagcgtcg
      901 ttgtacgacc tttattttcg ctaccgataa atccctgggt gatcactaag ccttcattga
      961 ggcgtgggag cagctgcagc gcggccagtt ccgccagcgc ggctacatct ggctctgcac
     1021 gaccaaatcg gtcgttggta cgcatcactt tacgtacatc aaaccactgt gcctgaacat
     1081 cgcgttcgcg caggatttcg acaaacagca gggtcgacat cagctcgccg tggctgacca
     1141 gctcatctgt cagcgccgga gacgttgcca gcgccgccgc ttctgccaga acagtaatgt
     1201 tctccagcag acgttcaatc tcttcacgga taacgttcgg gtaacgcaga cgttccagaa
     1261 tggcaaactg gatgttgcga atagcgtcga gtttttcgaa tcgctcgcca ggttccagcc
     1321 cttccgctaa agcgaccagc agattagtga taccagcaga agccgagagg actactaaac
     1381 gcacgttggc atcagaaagc acaatatcag cgctgcggtt catggcgtca aaatcagcta
     1441 cgctggtacc gccaaatttg gagacaacaa tttcagacat aactacctcg tgtcagggga
     1501 tccattttca gccttggcac aagggaagag cggaagacgg gtgggcgcag agcgatactt
     1561 cgctactatt ttcacccaga agtgctccac cacttgcgaa acgcccgact gcgaacgctt
     1621 ctggtgacaa cccaggggat tcagcccctg tagccgatga tgaacgtggc cagccgttca
     1681 atcacctcgg cgatgcaccc cctcaggtgt tatcacagga ctggctcctc caacaccgtt
     1741 acttgggcaa cgcgcctctt ctggcctgcg ctagcgcagg tagtacattt ataaataaag
     1801 ggtgagcggg gcggttgtca acgacggggt catgcggatt tttcatccac tcctggcggt
     1861 cagtagttca gctaataaat gcttcactgc gctaagggtt tacactcaac attacactaa
     1921 cggcactaaa accatcacat ttttctgtga ctggcgctac aatcttccaa agtcacaatt
     1981 ctcaaaatca gaagagtatt gctaatgaaa aacatcaatc caacgcagac cgctgcctgg
     2041 caggcactac agaaacactt cgatgaaatg aaagacgtta cgatcgccga tctttttgct
     2101 aaagacggcg atcgtttttc taagttctcc gcaaccttcg acgatcagat gctggtggat
     2161 tactccaaaa accgcatcac tgaagagacg ctggcgaaat tacaggatct ggcgaaagag
     2221 tgcgatctgg cgggcgcgat taagtcgatg ttctctggcg agaagatcaa ccgcactgaa
     2281 aaccgcgccg tgctgcacgt agcgctgcgt aatcgtagca ataccccgat tctggttgat
     2341 ggcaaagatg ttatgccaga agtcaacgcg gtgctggaga agatgaaaac cttctcggaa
     2401 gcgattatct ccggtgagtg gaagggttat accggcaaag caatcactga cgtagtgaac
     2461 atcgggatcg gcggttctga cctcggcccg tacatggtga ccgaagcact gcgtccgtac
     2521 aaaaaccacc tgaacatgca ctttgtttct aacgtcgatg ggactcacat ctcggaagtg
     2581 ctgaaaaaag taaacccgga aaccacgctg ttcctggtag catctaaaac cttcaccact
     2641 caggaaacca tgaccaacgc ccatagcgcg cgtgactggt tcctgaaagc ggcaggtgat
     2701 gaaaaacacg ttgcgaaaca ttttgcggcg ctctcaacca atgccaaagc cgttggcgag
     2761 tttggtattg atactgccaa catgttcgag ttctgggact gggtcggcgg ccgttactct
     2821 ttgtggtcag cgattggcct gtcgattgtt ctctccatcg gctttgataa cttcgttgaa
     2881 ctgctttctg gcgcacacgc aatggacaag catttctcca ccacgcctgc cgagaaaaac
     2941 ctgcctgtcc tgctggcgct gattggcatc tggtacaaca atttctttgg tgcggaaact
     3001 gaagcgattc tgccgtatga ccagtatatg caccgtttcg cggcgtactt ccagcagggc
     3061 aatatggagt ccaacggtaa gtatgttgac cgtaacggta acgttgtgga ttaccagact
     3121 ggcccgatta tctggggtga accaggcact aacggtcagc acgcgttcta ccagctgatc
     3181 caccagggaa ccaaaatggt accgtgcgat ttcatcgccc cggctatcac ccataacccg
     3241 ctctctgatc accaccagaa actgctgtct aacttcttcg cccagaccga agcgctggcg
     3301 tttggtaaat cccgcgaagt ggttgagcag gaatatcgcg atcagggtaa agatccggca
     3361 acgcttgact acgtggtgcc gttcaaagtg ttcgaaggta accgcccgac caactccatc
     3421 ctgctgcgcg aaatcactcc gttcagcctg ggtgcgttga ttgcgctgta tgagcacaaa
     3481 atctttactc agggcgtgat cctgaacatc ttcaccttcg accagtgggg cgtggagctg
     3541 ggtaaacaac tggcaaaccg tattctgcca gagctgaaag atgataaaga aatcagcagc
     3601 cacgatagct cgaccaatgg tctgattaac cgctataaag cgtggcgcgg ttaatcatca
     3661 tgcgtgcctg atgccgatat gtaggccgga taaggcgttt acgccgcatc cggcaaccgg
     3721 agcctgatgc caatatcgac attaatgcat agtatgagga gttaaataac cttaaaaaca
     3781 atgccgacaa taagtcggca ttgttttatc agataaatcc ccttgtctgt gatttaacgg
     3841 aaatcatacc
//
LOCUS       DAZPCS010000240         3828 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZPCS010000240 DAZPCS010000000
VERSION     DAZPCS010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3828
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.245"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            68..1402
                     /gene="adeQ"
                     /locus_tag="ACHUQ7_003861"
                     /gene_synonym="yicO"
     CDS             68..1402
                     /gene="adeQ"
                     /locus_tag="ACHUQ7_003861"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HFR1137046.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            1455..1907
                     /locus_tag="ACHUQ7_003862"
     CDS             1455..1907
                     /locus_tag="ACHUQ7_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFR1137047.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            2118..3308
                     /gene="nepI"
                     /locus_tag="ACHUQ7_003863"
     CDS             2118..3308
                     /gene="nepI"
                     /locus_tag="ACHUQ7_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HFR1137048.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(3349..3642)
                     /gene="yicS"
                     /locus_tag="ACHUQ7_003864"
     CDS             complement(3349..3642)
                     /gene="yicS"
                     /locus_tag="ACHUQ7_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFR1137049.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPHASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
BASE COUNT          779 a          860 c         1016 g         1173 t
ORIGIN      
        1 cactgaaccg ttgttgtcat ttgttttaca aaagcaaggg attaaatctc ctccaatgga
       61 caaaaaaatg aataatgaca ataccgatta cgtgagtaat gaatcaggga cgctttcgcg
      121 attatttaaa ctacctcagc atgggaccac cgtccgcaca gaattgattg cggggatgac
      181 cactttttta accatggtgt acattgtttt tgtgaacccg caaatcctcg gcgcggcaca
      241 aatggacccg aaagtggtgt ttgttaccac ctgtttgatt gccggtatcg gcagtattgc
      301 gatggggata tttgctaact tacccgtggc gctggctccg gcaatggggc tgaacgcctt
      361 ctttgcgttc gtggtcgtgg gggcgatggg gatctcctgg cagaccggga tgggcgcgat
      421 attctggggc gcaattgggc tatttttgct cacgctgttt cgtatccggt actggatgat
      481 ctccaacatt ccattaagtt tacgtattgg tatcaccagt ggaatcggat tatttattgc
      541 cttaatggga ttaaaaaaca ctggtgttat tgtcgccaat aaagacacgc tggtgatgat
      601 tggcgattta agttctcacg gcgtgttgtt aggtatttta gggtttttta ttataaccgt
      661 gttgtcatca cgtcattttc atgccgcggt gctggtttct attgtggtga cgtcttgctg
      721 tggattattt ttcggtgatg ttcattttag cggcgtctat tccattccgc ctgatattag
      781 cggcgtcatt ggtgaagtag atttgagcgg cgcgttaaca cttgaactcg ccggtatcat
      841 tttctccttt atgctgatca acctatttga ttcatcagga acattaattg gtgtaactga
      901 taaagcgggc ttaatagata gcaacggtaa attccccaat atgaataagg cgctgtatgt
      961 tgatagcgtc agttcggtgg caggcgcatt tatcggtacc tcgtctgtta ctgcctatat
     1021 tgaaagtact tctggtgtgg cagtcggtgg tcgcacgggg ctaacggctg tcgtggtcgg
     1081 cgttatgttc ctgttagtta tgttcttctc accgctggtg gcgatggttc ctccttacgc
     1141 aaccgctgga gcgttaatct ttgttggcgt gctgatgact tcgagcctgg cgcgcgttaa
     1201 ctgggatgat tttaccgaat cggtgcctgc gtttattacc acggtgatga tgccctttac
     1261 tttctcgatc accgaaggga ttgcactcgg ctttatgtcg tactgcatca tgaaagtgtg
     1321 caccgggcgc tggcgcgatc tgaacctgtg tgtggtggtg gtcgcagctc tgttcgcatt
     1381 gaagattatt ctggtggatt agtaactggc gagggtttcc agatatcatg agttctgatt
     1441 acgcaggaga actcatgatc tggataatgc tcgccacgct ggcggtagtg tttgtggttg
     1501 gttttcgggt gctgacatcc ggggccagaa aagcgattcg ccgtctcagc gatcggctga
     1561 atatcgatgt cgtacccgtg gagtcgatgg tcgatcaaat gggaaagtca gccggtgacg
     1621 aatttttacg ttatttgcat cgcccggatg agtcgcacct gcaaaacgcc gcgcaggtgt
     1681 tgcttatctg gcaaattgtc attgtcgatg atagcgaaca gaacctgctg caatggcacc
     1741 ggattttaca aaaagcccgc ctttccgcgc cgattaccga tgctcaggtc aggctggcgc
     1801 tgggttttct gcgtgaaacc gaacctgaaa tgcaggatat taatgctttt cagatgcgct
     1861 ataacgcgtt ctttcagcct gccgagggcg ttcactggtt gcattgaaac cgtttcgtct
     1921 tatcagccta cagaccgcgc ctgacctgtc gggtggataa gacgttcacg ccgcatccgg
     1981 taatctttgc cctgttccgt ttcgatctta actccagctc aagcaacacc gtaaaagttg
     2041 caaaaacgtt aaattcgtca cacatttcaa tgttaaagtg gtcggctttt cccctgaaac
     2101 atgccacggg taacaccatg agtgaattta ttgccgaaaa ccgcggcgcg gatgccatca
     2161 cccgaccgaa ctggtcagcc gttttctcgg tggcgttttg tgtcgcctgt ctgattatcg
     2221 ttgagttttt gcccgtcagt ttgttgacgc caatggccca ggatttaggc atttcggaag
     2281 gggttgccgg gcaatcggtg accgtgaccg cctttgtggc aatgtttgcc agtttgttta
     2341 ttacccagac aattcaggcc actgaccgcc gctacgttgt tattttgttt gccgttttgc
     2401 tgacgctctc ctgcttgctg gtttcctttg ctaactcatt cagtttgctt ttaatcggtc
     2461 gtgcctgtct ggggctggcg ctgggcgggt tctgggcgat gtcggcgtcg ctgaccatgc
     2521 gtctggtacc gccgcgtacg gtgccgaagg cgctgtcggt gatcttcggc gcggtttcta
     2581 ttgcgctggt gattgccgcg ccgttgggca gttttttagg cgagcttatc ggttggcgca
     2641 atgtctttaa tgcggcggcg gtgatgggcg tgctgtgtat tttctggatt atcaaatcat
     2701 tgccttcact gccaggcgaa ccctcgcatc agaaacaaaa tactttccgc ttattacaac
     2761 gtccgggtgt gatggcaggg atgatcgcca tcttcatgtc tttcgccggg cagtttgctt
     2821 tcttcacgta tattcgcccg gtgtatatga acctggcggg attcggcgtg gatggcttaa
     2881 cgctggtgct gttgagtttt ggtatcgcca gctttattgg tacgtcgctt tcgtcgttca
     2941 ttcttaaacg ttcggtaaaa ctggccttag caggcgcgcc gttaatactg gctgtgagtg
     3001 cgttggtact gacgttgtgg ggaagcgata aaatcgttgc taccggcgtg gcgattatct
     3061 gggggctaac ttttgcattg gttcccgtcg gctggtcaac gtggatcacc cgctcgctgg
     3121 ccgatcaggc agaaaaagcc gggtctattc aggtggcggt tattcagctt gctaatacct
     3181 gtggcgcggc aatcggcggt tatgcgctgg ataatattgg tctgacttcg ccgttgatgt
     3241 tgtccggcac attgatgttg ctgactgcat tgttggttac tgcaaaggtg aaaatgaaga
     3301 aatcctgata tcaggtaaag aagcgttggt cagtagttgt tgctgttctt acatatccgg
     3361 gcattctacc ttacccaacg cttcccaata attgctctga ttattacgtt ccatcgccac
     3421 gatggcctcg cgtatatgtt gtttattgtt ttcatctgac aacattaact tttcccacgc
     3481 ttcatctgat aatgacgcat gtggcgtgca aattttacgt aaatcttgta ataccgtggt
     3541 tttctctttt gcggactgca gtgagctaaa aggcgactcg gcgtatacga tgcctggcat
     3601 agcgaaaaaa gtgaaaataa gcagtagtgt cgttggtttc atggattcaa ccttttacaa
     3661 atgtgtatcg aatgaagcat aagtgtagtt tgctttcatc gctctcatca tgctgtacga
     3721 aacgcgcttg cgtggtcagt aagaagtgcc agactttata ttccactttt attccttttt
     3781 attcttatcg atagcgtttc gttttttaaa ccgcagcgac cttaccgc
//
LOCUS       DAZPCS010000241         3812 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZPCS010000241 DAZPCS010000000
VERSION     DAZPCS010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3812
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.246"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            425..1576
                     /locus_tag="ACHUQ7_003865"
                     /pseudo
     CDS             425..1576
                     /locus_tag="ACHUQ7_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEc16 family transposase"
     gene            complement(1606..2034)
                     /locus_tag="ACHUQ7_003866"
                     /pseudo
     CDS             complement(1606..2034)
                     /locus_tag="ACHUQ7_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            complement(2549..3592)
                     /locus_tag="ACHUQ7_003867"
     CDS             complement(2549..3592)
                     /locus_tag="ACHUQ7_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR1137050.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
BASE COUNT         1053 a          850 c          890 g         1019 t
ORIGIN      
        1 taaaactgag aaggagaaaa taattgagta actaaaagta ctcaggccat aagtatgcat
       61 gaaaattgat ggtgatattg atatgtagca aaatagagaa ctgagtatta atgagacaag
      121 tatagtgccg cagataaact ctttggtcac aaaggatgca ctgttttgac atattacttt
      181 ctcatttgca tgagttatct ttggtgataa taacattatt ataatactga acagcatcat
      241 aacaagaaat ataacattcc agttataata ttcaataatc aatgcgcgag gtgctactaa
      301 tggtattact gcgcttacag atgatatatc agaaagcgcc cgttttagtt ttaatggagg
      361 gtgatcttac ccaccaatag tggacacagg actaagtgag taaactctca accagaggtg
      421 actcatgaca aaaccagtat caatcagcaa gaagccccgt aaacaacata cgcctgaatt
      481 tcgtaacgaa gccctgaaac tcgctgaacg catcggtgtg gccgccgcag cccgtgaact
      541 cagcctgtat gaatctcagc tttatgcctg gcgcagtaaa cagcagcaac aaatgagttc
      601 gtcagagcgc gaaagcgaac tggccgctga aaatgtccgc cttaaacgac aactggcgga
      661 gcaggctgag gaactgtcca tcctccaaaa ggccgcgaca tacttcgcga agcgcctgaa
      721 atgaagtatg tctttatcga aaatcatcgg gcagagttca gcatcaaagc gatgtgtcgt
      781 gtacttcggg ttgcccgcag cggctggtat gtctggctca ggcgtcgtca ccagatgagc
      841 ctgcgccaac agtttcggct cacctgcgat gccgctgtcc ataaggcatt ctttgaggca
      901 aagcagcgat acggtgctcc ccgccttgct gacgaactgc cggagttcaa tattaaaacc
      961 attgccgcca gcctgcgtcg tcaggggctg cgggcgaaag ccagccggaa gttcagcccg
     1021 gtcagctacc gtgcacatgg cctgcccgta ttggagaatc tgctggagca ggacttcagc
     1081 gccagtggcc cgaaaccaga agtgggcggg tgacatcacg tacttgcgta ccgatgaggg
     1141 ctggttgtat ctcgcagtag tcatcgacct gtggtcacgc gccgtcattg gctggtcgat
     1201 gtcaccgcga atgacagcac aactggcctg tgatgcactg caaatggcgt tgtggcggag
     1261 aagacgcccg gaaagcgtca ttgttcatac ggaccgtggt ggtcaatact gttcagggga
     1321 ttatcaggca atgctgaagc gacacaacct gcgtggcagt atgagtgcga aaggctactg
     1381 ttatgacaat gcctgtgtgg aaagcttctt tcattcgctg aaggtggaat gtatccacgg
     1441 ggaacgcttt agcagccggg aaataatgcg ggcaacggtg tttaattata tcgagtgtga
     1501 ttacaatcgc tggcgtcgtc acagtgcctg tggcggtctc agcccggaac aatttgaaaa
     1561 ccataatctc gcttagggcc gtgtccacat tacgtgggta ggatcagtat gggtcgtctt
     1621 gtatttgtca tactgcccaa tctcaccaat cgaaaatgct ccttttggca aaggttcaag
     1681 caggtctctg ggaatggagg tcgctggcgt cggatataaa aaaccctcac ggcttagcaa
     1741 ggctggcgtc actatcggtg taacaatatc aggcgtgcct gcggaacggt ccttaccatg
     1801 ctgcagtaac cggaaataaa cttttgggcg taagatgaca ttgacagttt cgacatcgtc
     1861 tttttctccc ttaaaaaatt tattgacaat cgcttcgtca agacgtcccg ccgctatccc
     1921 atgccagtga gtgaaatttt gggcgtcttt ccgttcaaaa cagccctttc ctgactcagc
     1981 atctgcaagc gagttgcgcc agtatgaggc aaaccttaaa gcattttcat ccattaccag
     2041 tcctgtgact caatgaaatc cattgattta tcgaatataa aaccaccgta tggacaacta
     2101 ttgtccatat taaaaaagtt ggatgcaatg ttttgtttat aattcgatat caaatgtact
     2161 ggttgtacga ggttcagaac acaagcacat tcaacagtac gtcaggacta ttgatttcat
     2221 aacagcgaat acaatttttt actgtttata atggctgcct gtgttggacg gggataccag
     2281 ttcaaccgaa aacgccagag gaggggatta cccgctggca gggtaaatct gtggtttttg
     2341 atgccagcta taacgtgttt gacgataaag tattacgaaa gaactttaat gagttcatgg
     2401 agtaattcgt taaagtaatt tacaacgcta tccttttgca aaggaaactt tcagaaaata
     2461 accccagggt aatcacctgg ggaaggaaca cagtgttatt attggataat aaattattac
     2521 ttgttttaaa gttacactaa tgcaatatct actgatactg gatgcttgaa tgtttcatcc
     2581 ctgctgcgga aaagggcaat atccagggcc agatatattc accgttcata tttctgcatt
     2641 tctgtacaat ttcctcagaa agtgtcttgt caattctttt ccacaggtgc tcctgctgtc
     2701 ttaaaatact ttcgcatatt tcgaaatcat cttcctgaat cagcatttcg gcgcatttaa
     2761 tgatttctga tcgttcatgc agcacggagg aatggttata gagccagttt acaaacagca
     2821 atgcttccgg atattgtccc cataatggtg agtatgttgc atagtaatct tctgccgtat
     2881 ccagagagcc ggagaagtca tgccacacag caagtggaaa tggatgcaga tttccgcaca
     2941 gcatttctgc gatgtaagtg tttgtggcaa agccatggcg aaaagctgtg gcggccttaa
     3001 catgttcccc gttcagaaaa tgcaggagac cgagttcata ccagtaaggt gaaaattcat
     3061 cagcatgctc tttcagtaca ctgaaggctc gctcatgatc gccggtgcgt aacagctctg
     3121 aacccaacaa ccagcgagcc ccctgattat cttcaggatt gtatgccagt attttatcag
     3181 tgagcataac cgcatcctga tggcgctgta agtgcacatt tgcgagaatt gctgcataaa
     3241 gcgcgcgcag ataaggccgg ttttcgggat gcatccagat tatttcacca cagaaacttt
     3301 ccgggatgat acgattacct gccgccagtc ctttaagagc ggcgttcagt gccttacgcg
     3361 gggcattctg ttccagaaat gcatatgcca gatgagcgtg tatatcaata aagtccggct
     3421 cccgttgagc aaggcgtcgg agtttgttga tgtaactcgt ctcgctgatt tcatcagcgt
     3481 cgtatgcatc aagcacttct tcaaactccc ccagcaatgg gccaaaccag gtatccggaa
     3541 atacgaaata acccttgtta tcgtgcactt caaagcataa tctatcgttc attttctgag
     3601 cctgtaaaac atgtggtgat atagtctgag agcataaaag ttgagattct gtgtatcaag
     3661 tcctgtcttg gcaccgaatg aatattgctt acgaagcccc ttcatgcttt ccttgagaac
     3721 aataatttta tctttatgtg aatctcattt tttataatga tgtcagatga ttgaaatgct
     3781 aaattatatc tgaatcaact attcctttgc ta
//
LOCUS       DAZPCS010000242         3807 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZPCS010000242 DAZPCS010000000
VERSION     DAZPCS010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3807
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.247"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            437..2090
                     /gene="ushA"
                     /locus_tag="ACHUQ7_003868"
                     /pseudo
     CDS             437..2090
                     /gene="ushA"
                     /locus_tag="ACHUQ7_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar hydrolase/5'-nucleotidase
                     UshA"
     gene            complement(2101..2271)
                     /locus_tag="ACHUQ7_003869"
                     /pseudo
     CDS             complement(2101..2271)
                     /locus_tag="ACHUQ7_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2404..2523)
                     /locus_tag="ACHUQ7_003870"
                     /pseudo
     CDS             complement(2404..2523)
                     /locus_tag="ACHUQ7_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2597..3596)
                     /locus_tag="ACHUQ7_003871"
                     /pseudo
     CDS             complement(2597..3596)
                     /locus_tag="ACHUQ7_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421714.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT         1053 a          878 c          874 g         1002 t
ORIGIN      
        1 aatcgacgat tgctggaaag gctgggccac atccctccgg cagaagcaga aaaagcttat
       61 tatgcttcta tcggaaacga tgatctggca gcctgagttc acagataaaa cattctccag
      121 gaaacttggg gcggttcacg tcagtatcag accatattaa ttgctgaaac tgataaacat
      181 caaaccttat agaagggtga atgctgaaag ggggctatgg tgatcgacgt gatgacaacg
      241 acaggtaaga ccgtgacctg ataaacctga attgtgtcct agacataaag tccgtgaagg
      301 gaataaggct caggtcagcg aatcttatag tggcccgcag ctctgactgc aataaggccg
      361 gatacagagc agcaactact cgtcgatgtc agaacaacgg aagccttgca aacggggatg
      421 cgttcataca ggaacaatga taccgttaaa aaaaaacatt acgctaataa tgttcaccct
      481 gtcattactg acaggaaatc ctgctatagc gtatgaaaca gataaagtat ataaaataac
      541 cgttctgcat acaaatgacc accatggaca cttttggcgt aacaatcatg gtgagtacgg
      601 gttatcctcc caaaaaactc tggtggacaa tatccggcag aaagtaataa ataatggcgg
      661 aagtgttcta ttattatctg gtggtgatat taacaccggt gtacctgaat cagaccttca
      721 gaaggctgaa cccgatattc gtggaatgaa tcataattgg ttatgatgca atggccgtcg
      781 gtaatcatga atttgataat cctctaaata tattacgtca gcaggaaaaa tgggccacat
      841 ttcctttcct ttctgctaac atatatcaaa aaagtacagg gaggcgttta ttcagtccat
      901 ggaaaatttt catccgtcag aatttgaaaa ttgcagtcat tggcttgacg acagatgata
      961 ctgcgaaaac aggaaattct gaatatttta ctgatattga gttccgtcaa cctgctgctg
     1021 aggcgaggag tgtcattgat gaacttaatc agcaggaaaa gccagatatt attattgcgg
     1081 caacacatat gggtcactat gacaatggcg agtcaggttc gaatgctcct ggtgatgttg
     1141 aaatggctcg cagtttgccg actggctcat tagcaatgat tgtgggagga cattcacaag
     1201 ctcctgtctg catggccagc gataataaaa aacagtggaa ttatattccg gggacaacct
     1261 gtgtaccaga taaacaaaat ggcatctgga ttgtacaagc gcatgagtgg ggaaaatatg
     1321 tcggtcaggc tgattttgaa ttctgtaacg gtacaatgaa actggttaat tatcagttac
     1381 acccagttaa tctcaaaatg agaattacca gagaagatgg aaaaacggaa ttttcttttt
     1441 acacccccga aattactgaa gatccacaga tgttatcctt actgacacca ttccagaata
     1501 aaggcaaagc acaactggat gtgaaggttg gggttgtgaa cggacgtctg gaaggggaca
     1561 gaagtaaagt tcgttttgtt cagaccagta tgggccatct gattctgagc gctctgacag
     1621 aacgtattga cgcagatttt gctgttgtca gtggtgggga aattcgagat tccatagaga
     1681 gtggtaacat cacatacaaa gatatactga aagttcagcc atttggtaat acggtagtaa
     1741 gtattgattt gacggggaaa gaagtagctg actacctagc tacagtggca cagatgaaac
     1801 cggattccgg agcctaccca caatttttga ataccagctt tgtagtcaaa aaaggaaaaa
     1861 tcgaaatgct aaaaataaaa ggtaaatcgg ttgaccttaa caaaaaatat cgtatgacaa
     1921 catttagctt taatgctact ggtggggatg gctatccacg tattgataat agacccggat
     1981 acataaatac cggatttatt gatgctgaag tacttatcga atatatccgg aaacattcac
     2041 cgctggatgc tgcttcatat gaaccaaaag gtgaagtcag ttggcagtga taacgtaatt
     2101 gacgatcatc acgtgttttt tgctgacagt cgctgtgact ttgtgaaagt agtctctgcg
     2161 tatgtttgcg atatgggaat gcagttgctg tattttgtgt ttctgcttct gccagttgtt
     2221 gctgaattta accttgcggc ttaactgatg ttggagcgct gccagcttct tcgtcaattt
     2281 cgctgattaa agtttattca ttatggcatg atctgcgtaa gcggctcgtc agaaccatat
     2341 tgatatttac taagagctca gatcaacttt ccagggcaac agatcgcgta cccggtttgc
     2401 cggtcagtcc tggatatgct caatgacgta acgcagccat ttttctggct ccacattgtt
     2461 cagacggcat gtgccgatca gcgagtacaa caccgccgca tgttcgccac cgctatcgga
     2521 accccgccgg agagagtttc actctccggc tgctcacgga accgtacgtg acactctcgc
     2581 gtcatacggc tcttgatcac tcattcgatg cacgtagtaa ataccagtgg gcaaatagct
     2641 tcggatactg tttccttacc agcgccagct ttcgctgagc cttccggttc gaaagtctgt
     2701 atttagactg catccactta agcaaacgtg actgcattgc actccacagt cgatagctga
     2761 agtttctgga ccagaacttt ccatagtact caatccagcc tctcactatc gcattgtagc
     2821 gcctcgcaaa atccagcaaa ctctcagctg ttgagcgatg tatacgccac ttcttgattg
     2881 tttctgttat tttgcacatt gctgcatttg atgcacccgg catacatttt ctgtacaatt
     2941 cgcctttgaa attcttcaga gtacgcactt tgaagtcata tccgagagtg gcatacaaca
     3001 tcatcggcgt acctttcgaa gggaacgccg ggaaactgac gttgcatcca catatcaaag
     3061 gcatagtgca ggaacagatt tgagagcagg ggggagatta cgccgccctg cggcgttcct
     3121 cgaccccgtg ccagcattgc tccatccggc atttgcaccg gtgccttcag cctgcgctca
     3181 atgtacatca ccacccagcg tgcctgagta tgatgctgca ctgtcttcag tagcaggtca
     3241 tgatcgatat catcaacgaa gcctttaata tcgacttcca ccacccagtc aaacttccag
     3301 cagcgtttcc tcgcctgcgc aatcgcctgg tgtgcggatt tacccggtcg ataaccatac
     3361 gagtctgcat ggaacagggg ttccagtacc ggctcgagta tctgcttaac gaccatctgg
     3421 gcgatccggt ccgacacggc cggaataccg agcggacgta caccaccgtc agctttggga
     3481 atgtccacac gccttaccgc ctgcggcata taactgccgg aagccattct gttccacagt
     3541 ttgtagagat tgtctgtaac attctcgtca aacccggcca gtgtctgccc gtcaatgcct
     3601 gccgccccac ggttggcttt tactcgttta taagcttccc atacctgtcg tttacttaca
     3661 ggaaatggct ttgccgattt tacagcagtc ctcccatcgg cattgtcgcc cgtggttgcc
     3721 gccgcaccat cagcacaatg gggcaagccc tttgctccgg acccattacg gtccttcacc
     3781 gctactacgg tttactccgt ccctgta
//
LOCUS       DAZPCS010000243         3802 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZPCS010000243 DAZPCS010000000
VERSION     DAZPCS010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3802
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.248"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            87..314
                     /locus_tag="ACHUQ7_003872"
     CDS             87..314
                     /locus_tag="ACHUQ7_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020232163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1137051.1"
                     /translation="MACNVFAFSISGGVSERSYKETEKTSAMTTTHSTKLQPSQAILF
                     KMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(391..633)
                     /locus_tag="ACHUQ7_003873"
     CDS             complement(391..633)
                     /locus_tag="ACHUQ7_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFR1137052.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            986..1906
                     /gene="lpxP"
                     /locus_tag="ACHUQ7_003874"
     CDS             986..1906
                     /gene="lpxP"
                     /locus_tag="ACHUQ7_003874"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFR1137053.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            2261..2332
                     /gene="ypdK"
                     /locus_tag="ACHUQ7_003875"
     CDS             2261..2332
                     /gene="ypdK"
                     /locus_tag="ACHUQ7_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFR1137054.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(2397..3635)
                     /gene="alaC"
                     /locus_tag="ACHUQ7_003876"
     CDS             complement(2397..3635)
                     /gene="alaC"
                     /locus_tag="ACHUQ7_003876"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFR1137055.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKCYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENHDRIRQAIRGIKA
                     MFRADGLLPVSSKHIHENAE"
BASE COUNT          985 a          963 c          884 g          970 t
ORIGIN      
        1 gtatggttct cagtgaacca ttttcaggat aactttctat gaaagtaaac ttaatacttt
       61 tcagcttatt tttattggtc tctattatgg catgcaatgt ttttgcattt tccatttctg
      121 gtggtgtaag tgagaggagc tataaagaga ctgaaaaaac atcagcgatg acgaccacac
      181 actctacaaa acttcagcca tcacaggcga ttttgtttaa gatgagagaa gatgtgccac
      241 cattaaacct cacagaagaa atcacgccta cttatccgac aaaggcgaat tatcttattc
      301 atcctgtgcg atagcgtctg tcacatggca ggtgaacaat catgtctgat gctgatgtga
      361 acacctgccc aaatgagcca tcctgaccca ctatcgcctg ccatacccca ccataaagcg
      421 aatcatcaag ccacaagccg gggcaagaaa cataaatatc cccgtcacca gttcattacc
      481 gccagttaac cacatggtga agtaataaat gagtgcagcg aggatcacca tgccgaagaa
      541 agaagagacc gccgatacca cgttcagtac ctgaccgatg tagccgctta cgcagacaat
      601 acacagggcg agaaacatcc ataaataaat catagctcaa cctcctgccg cgctaaaaaa
      661 cctgtaactt cagtcacttt aatagtaaga caatccttaa cctctggcaa tttttgctaa
      721 gcatcaatga aaacagcacg ttaaatgcaa cgctattgcg tgcggaaaat tttttacttt
      781 gcacgattaa taatcagtag ctgaaagcag tcagccagtg ataaagtaga gccaccgcat
      841 tcatgctcct cgtggttatg tcctgactag tctttacact ctttacagga accattgtcg
      901 tacatgatgg cccaaccaat tgagtgtacc gctgtcgaca tggaatcttc tgcgatacaa
      961 caattcgtat ctacagaagg taactatgtt tccacaatgc aaattttccc gcgagtttct
     1021 acatcctcgc tactggctca catggtttgg gcttggtgta ctctggcttt gggtacagct
     1081 tccttatcct gttctctgct ttctcggcac gcgtattggc gcaatggcgc gaccattcct
     1141 gaaacgtcgt gaatctatcg cccgtaaaaa cctggaactt tgtttcccgc agcattctgc
     1201 ggaagaacgc gagaagatga ttgccgaaaa ctttcgttca ctcggcatgg cgctggtaga
     1261 aaccggcatg gcatggttct ggcccgacag tcgcgtacgt aaatggtttg atgttgaagg
     1321 gttggataac cttaaacgcg cacaaatgca aaatcgcggc gtaatggttg tcggcgtcca
     1381 ttttatgtcg ctggaactgg gcggccgcgt gatgggactg tgccaaccaa tgatggctac
     1441 ctatcgtcca cataataatc agctgatgga atgggtgcag acccgtgggc gcatgcgctc
     1501 taacaaagcg atgatcggca gaaataatct gcgcggcatt gtcggtgcac tgaagaaagg
     1561 tgaagcggta tggtttgctc ccgatcagga ttatggtcgt aaaggcagct ccttcgcgcc
     1621 gttctttgcg gtggaaaatg tcgccacaac caatggcacc tatgttctct cccgtctctc
     1681 tggcgcagcc atgttgaccg taacgatggt aagaaaagcg gattacagcg gatatcgttt
     1741 gttcatcacc ccagagatgg aaggctaccc gacagatgaa aatcaagccg ctgcttatat
     1801 gaacaagatt atcgagaaag agatcatgcg cgcaccggag cagtacctct ggatccaccg
     1861 tcgctttaaa acgcgcccgg tgggagaatc gtcgttgtac atttaacaaa ctgctcttac
     1921 tctttccctc ctcaaatcgg ccataataac ctcatgattt ttatagcttt tttcttaagt
     1981 tttttccttc gctgaatatc taattattac cgtcagaaat taaactgtcg ccgatatgag
     2041 acacttgtaa gttactaaaa gtaaataaga aaatgcctct tgtctcgttt cagttttagg
     2101 cgtacattag cgccgtctcg agcagtttag ctcagaattc tggggtgatg cccacggtaa
     2161 aatacagggc ttttaaggtc gctttgcccg gtgtcaactc actgtatcag gtgtaatgaa
     2221 gtcattcagg cgtaacagta attacgcgga gagatgtaaa gtgaaatatt tctttatggg
     2281 catttctttt atggtcatcg tttgggccgg tacttttgcc ctgatgatct aaaagcaaac
     2341 tgtgcaatga aaaacaggag ccatcaggct cctgtttgca tttctgacga cggtgtttat
     2401 tccgcgtttt cgtgaatatg tttgctgctg acgggtagta aaccgtcggc acggaacatc
     2461 gctttaatcc ctctgatggc ctgacgaata cggtcgtggt tttcaatcag tgcaaagcga
     2521 acatgcgtat caccatagtc gccaaagcca atccccggcg agacacaaac cttcgcttcg
     2581 ttaaggagct tcttggcaaa ttccagcgat cccatggccg catatggttc cgggattttc
     2641 gcccagacat acatcgaagc cttcggcatt tcgaccatcc agcccgcttc atgcagccct
     2701 ttaaccaata catcacgacg gcgtttgtac tgttcggcaa tgtcgcgcac gcactgttga
     2761 tcgccctcga gtgccgcaat cgccgccacc tgcaacggcg taaaagtgcc gtaatcgtga
     2821 tagcttttga ttcgcgccag ggcactgacc agcgttttgt tgccgaccat aaagccgata
     2881 cgccagcccg ccatgttgta gcttttcgac agcgtaaaga actcgaccgc cacatcgcgt
     2941 gcacccggca cctgcataat tgacggcgct ttccagccat cgtagacgat atcggcatag
     3001 gccaggtcat ggaccaccag cacatcgtag catttcgcca gcgccacaac cttttcgaag
     3061 aactccagtt ccacgcattg cgcggttggg ttcgacggga agccgaggat catcatcttc
     3121 ggtttcggat aactttcacg aatggcgcgc tccagttcgt tgaagaaatc gacaccttcc
     3181 accagcggca ctgagcgtac ctgcgccccg gcaatcaccg cgccataaat atggatcggg
     3241 taacttggat tcggcaccag caccgtgtca ccatgatcca gcgtcgccag catcagatgc
     3301 gccaggccct ctttcgaacc aatagtgacg atggcttctg attccgggtc gatttcaacg
     3361 tcgtagcgat cctgatacca gcgggaaatg gcgcgacgta accgcggaat gccgcgtgaa
     3421 gtggagtaac catgcgtgtc cgggcgctgg gccacagtac ataatttttc gacgatatgc
     3481 ggcggagtcg caccgtccgg gttacccatg ctgaaatcga taatatcttc gccgcgccga
     3541 cgcgcagcca ttttcagttc agcggtgatg ttaaaaacgt agggcgggag acgatcaatg
     3601 cgcgtaaagc gacgttcagg gcgagtgtca gccataattt cctcagatta acgttagcgc
     3661 ccggaccgtc cgagcgacgc tgccacaatg gtggctgttt tgaaaatagc ctgattaatt
     3721 tctgtctgtc cagcgtttta gaacattttt atcaatggat ttaaatgagg aactccactt
     3781 caaaaacagg aaatttttct ac
//
LOCUS       DAZPCS010000244         3793 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZPCS010000244 DAZPCS010000000
VERSION     DAZPCS010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3793
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.249"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            2..256
                     /gene="yoeB"
                     /locus_tag="ACHUQ7_003877"
     CDS             2..256
                     /gene="yoeB"
                     /locus_tag="ACHUQ7_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HFR1137056.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            339..1163
                     /locus_tag="ACHUQ7_003878"
     CDS             339..1163
                     /locus_tag="ACHUQ7_003878"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR1137057.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            1209..2192
                     /locus_tag="ACHUQ7_003879"
     CDS             1209..2192
                     /locus_tag="ACHUQ7_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR1137058.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAIQRKIIMTSLSFSCHFPPI"
     gene            2353..2415
                     /gene="yoeI"
                     /locus_tag="ACHUQ7_003880"
     CDS             2353..2415
                     /gene="yoeI"
                     /locus_tag="ACHUQ7_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFR1137059.1"
                     /translation="MGQFFAYATVITVKENNHVA"
     gene            2405..3763
                     /gene="plaP"
                     /locus_tag="ACHUQ7_003881"
     CDS             2405..3763
                     /gene="plaP"
                     /locus_tag="ACHUQ7_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFR1137060.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANVNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
BASE COUNT          892 a          897 c         1001 g         1003 t
ORIGIN      
        1 agtgaaacta atctggtctg aggaatcatg ggatgattat ctgtactggc aggaaacaga
       61 taagcgaatt gttaaaaaga tcaatgaact tatcaaagat acccgcagaa caccatttga
      121 aggtaagggg aaaccagaac ccctgaaaca taatttgtca ggtttctggt cccgacgcat
      181 tacagaggag caccgtctgg tatacgcggt tactgatgat tcactgctca ttgcagcatg
      241 tcgttatcat tattgaacag attcattcat agaatgaatt gtcagttttg atacgctagc
      301 gtagccttat agatctaagg tgtatcagga gataacggat gaaaaaggtc gcaattgtcg
      361 ggttagggtg gttaggtatg ccgctggcga tgtcactttc agcgcgaggc tggcaagtca
      421 ccgggagtaa aaccacacaa gatggtgtcg aagcggcccg aatgagtggc attgatagct
      481 atctgcttcg catggagcct gagttagttt gcgattctga cgatctggat gcccttatgg
      541 atgccgatgc gctggtcatt acgcttccgg cacgtcgtag cggccccggc gatgagttct
      601 atttacaagc ggtacaagag ttagtggata gcgcgctggc ccatcgtatt ccccgaatta
      661 tttttaccag ctcaacatct gtctatggcg acgcgcaagg cacggttaaa gaaactaccc
      721 cgcgtaatcc ggtaaccaac agtggacgag tattagaaga actcgaagac tggctgcaca
      781 atttacccgg tacttcggtc gatattctgc gtcttgcggg cctggtcgga ccgggacgtc
      841 atccgggacg cttctttgcc ggaaaaaccg cccctgatgg tgaacatggt gttaatttag
      901 tccatttaga agatgttatt ggcgctatca ctctgttgtt acaggcacct aaaggcggac
      961 acatctataa tatatgtgcg ccagctcacc ctgcgcgtaa tgttttctat ccgcagatgg
     1021 cccgtttact ggggctgcaa cctccgcagt tcagaaatag tctggacagc ggcaaaggta
     1081 agattattga tggcagtcgg atttgtaatg aactgggatt tgaataccag tatcccgatc
     1141 cgctggtaat gccgttggag taaacgatca cgccagcagg acgccgcatg cactgcaagg
     1201 gggcgtgtat gaaaccactg ctggatgtgt tgatgatcct cgatgccata gaaaaagagg
     1261 gcagttttgc ggcggcgtca gccaaactct acaagacgcc gtccgctctg agttacaccg
     1321 ttcacaagct ggaaagcgac ctaaatattc aattgcttga tcgtagcgga caccgcgcta
     1381 aattcacccg caccggaaaa atgttattag agaaagggcg ggaagttctg cataccgtgc
     1441 gagaactgga aaagcaggcg atcaaactgc atgaaggctg ggaaaacgag ctggtgattg
     1501 gcgtggacga cacgtttcct ttttctctgc ttgcgcctct cattgaagct ttttatcaac
     1561 atcatagcgt gacgcgcctg aagtttatca atggcgtact cggtggttcc tgggatgccc
     1621 tgactcaggg gcgagcggat attatcgttg gcgcgatgca tgagccacca tcttccagtg
     1681 agtttggttt ctcgcggttg ggcgatcttg aacaagtctt tgcggtcgcg cctcatcatc
     1741 cgttagccca ggaagaagaa ccgttaaacc gtcgtatcat taaacgatat cgggcgattg
     1801 tggtggggga tactgcgcag gcaggcgctt ctacggcctc acaacttctc gacgaacaag
     1861 aagccattac cgttttcgat tttaaaacca aactggagct gcaaattagc ggccttggct
     1921 gcggctattt accacggtat ctggcgcaac gttttctcga tagtggcgcg ttaatcgaga
     1981 agaaagtggt cgcccaaact ctctttgaac ctgtctggat tggctggaat gaacagaccg
     2041 cagggcttgc cagcggctgg tggcgagatg aaattttagc aaatagtgcg atcgccggtg
     2101 tttatgcaaa atctgatgac ggaaaatcag ccattcaaag aaaaattatt atgacaagcc
     2161 tctcattctc ttgtcatttc ccccccattt aggcacaatg cgccgctgtc aaaaaatgat
     2221 taaaaaccga cgtttaatta gcgtcggtta ttttttgctt caaaccaatc attcatacca
     2281 agaggccggg cttcgtaccg gatagatatt tactaaaaat cgacagttgt tgtcgctgag
     2341 gaatccaaaa aaatggggca attttttgct tacgcgacgg ttatcaccgt aaaggagaat
     2401 aaccatgtcg cataacgtta ctccaaacac ctctcgcgtg gaattgcgta aaacgcttac
     2461 gttagttccg gttgtaatga tgggtctagc ctatatgcag ccgatgacgc tgtttgatac
     2521 atttggtatc gtttcaggcc tcacggatgg tcatgtgccg acagcctatg cgttcgcatt
     2581 gattgcgatc ctgtttacgg ctctgagcta cgggaagctg gttcgccgct atccttctgc
     2641 tggctctgca tacacttacg cccagaaatc cattagcccg actgtcggct ttatggtggg
     2701 ttggtcttct ctgctcgact atctgttcgc gccgatgatc aacattctgc tggcgaaaat
     2761 ctattttgaa gctctggtgc cttccattcc atcgtggatg tttgtggtgg cgctggtggc
     2821 ctttatgacc gcctttaacc tgcgtagtct gaaatccgta gcgaacgtca acactgtaat
     2881 cgtcgtgttg caggtagtgc tgatcgcggt gattctgggc atggttgttt atggcgtatt
     2941 tgaaggtgaa ggcgctggta cgctggcgag cacgcgtccg ttctggtctg gcgatgcgca
     3001 tgttatcccg atgattaccg gggcgacaat cctgtgcttc tcctttaccg gctttgacgg
     3061 catcagcaat ctgtcggaag aaaccaaaga tgcagagcgc gtgatcccgc gtgcgatttt
     3121 cctgaccgcg ctgattggcg gcatgatctt catctttgca acttacttcc tgcagctgta
     3181 cttcccggat atctctcgct ttaaagatcc ggatgcgtca cagcctgaaa tcatgctgta
     3241 cgttgcgggt aaagcgttcc aggttggcgc gctgatcttc tccaccatta ccgtactggc
     3301 gtccggtatg gcggcgcacg caggcgtagc gcgtctgatg tacgtaatgg gtcgtgacgg
     3361 cgtgttcccg aaaagcttct tcggttatgt acacccgaaa tggcgtactc cggcgatgaa
     3421 catcatcctg gttggcgcga ttgccctgct ggcaatcaac ttcgacctgg taatggcaac
     3481 ggcgctgatt aactttggtg cgctggtggc gttcaccttt gttaacctgt cggtcatctc
     3541 gcagttctgg atccgtgaga agcgtaacaa gacgctgaaa gatcacttcc agtatctgtt
     3601 cctgccgatg tgtggtgcgc tgaccgttgg tgcgctgtgg gttaacctgg aagaaagctc
     3661 aatggttctg ggtctgatct gggctgctat cggtctgatt tacctggctt gcgtcaccaa
     3721 gagcttccgt aatccggttc cgcagtacga agacgtagca taatcatctt ttctaataat
     3781 aaaccggagt cga
//
LOCUS       DAZPCS010000245         3691 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZPCS010000245 DAZPCS010000000
VERSION     DAZPCS010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3691
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.250"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            108..2105
                     /locus_tag="ACHUQ7_003882"
     CDS             108..2105
                     /locus_tag="ACHUQ7_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004119118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HFR1137061.1"
                     /translation="MSENDTIPKKSTSQINKAVFFTSALLIFLLVAFAAVFPDVADKN
                     FKLLQQQIFTNASWFYILAVALILLSVTFLGLSRYGDIKLGPDHAQPDFSYHSWFAML
                     FSAGMGIGLMFFGVAEPVMHYLSPPVGTPETVAAAKEAMRLTFFHWGLHAWAIYAIVA
                     LILAFFSYRHGLPLTLRSALYPIIGDRIYGPVGHAVDIFAVIGTVFGVATSLGYGVLQ
                     VNAGLNHLFGVPINETVQVILIVVITGLATISVVSGLDKGIRILSELNLGLALLLLAL
                     VLCLGPTVLLLKSFVENTGGYLSELVSKTFNLYAYEPKSSNWLGGWTLLYWGWWLSWS
                     PFVGMFIARVSRGRTIREFVTGVLFVPAGFTLMWMTVFGNSAIYLIMNQGATDLANTV
                     QQDVALALFNFLEHFPFSSVLSFIAMAMVIVFFVTSADSGAMVVDTLASGGVANTPVW
                     QRIFWASLMGIVAIALLLAGGLSALQTVTIASALPFSVILLISIYGLLKALRRDLTKR
                     ESLSMATIAPTAARNPIPWQRRLRNIAYLPKRSLVKRFMDDVIQPAMTLVQEELNKQG
                     TISHISDAVDDRIRLEVDLGNELNFIYEVRLRGYISPTFALAAMDNDEQQTEQHRYYR
                     AEVYLKEGGQNYDVMGWNQEQLINDILDQYEKHLHFLHLVR"
     gene            2168..3445
                     /locus_tag="ACHUQ7_003883"
     CDS             2168..3445
                     /locus_tag="ACHUQ7_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HFR1137062.1"
                     /translation="MISRWKWMLKQTIKKLWFRATLFAIVAIITALLSILFKSMIPES
                     VSVKVGAEAVDNILNILASSMLAVTTFSLSIMVTAYGSATTNVTPRATRLVVEDVTTQ
                     NVLATFIGSFLFSLVGIIALNMGAYGERGRVILFIVTLVVIALILITLLRWIQHLTSL
                     GRVGETTAQVEQAAIETFIARARNPCLGGYPWLENNEQPKGTVAVYPKKIGYVEYIDM
                     VKLSKLLTNDPRHVYLVAQPGSFIHPSMPVLYLSQGQESSINTDLLETIIVSDARSFA
                     QDPRFCLSVMAEIACRALSPAVNDPGTAIDVIGRGVRILSTYAQNKSDEIEVKYPSVH
                     VAPLQNNDLLEDFFSPVARDGASMREIQIRVLKGLSMLSKGWPEIFAEAAHTLAFETL
                     EHATRADHIDSDRYLIKSTYYNLFSGEYSNKKT"
BASE COUNT          892 a          839 c          930 g         1030 t
ORIGIN      
        1 ttccaccatg cattacgatt aacgccgcat agccagttga actttgctac tttgtgagag
       61 gtagtacctt ctatccagtg cgaatttaat taatggaata aatgattatg agtgaaaatg
      121 atacaatccc aaagaagtct acaagtcaga ttaacaaagc ggtattcttt acatctgctt
      181 tgctaatttt ccttcttgtc gcctttgccg ccgtattccc ggatgtcgcc gacaaaaatt
      241 ttaaactact tcagcaacaa atcttcacga atgccagctg gttctacatc cttgctgtgg
      301 ccctgatttt actgagtgtc acgttccttg gactctcacg ctacggtgat atcaagctgg
      361 gcccggacca tgcgcagcct gatttcagct accactcctg gtttgcgatg cttttttcgg
      421 cagggatggg gatcggcctg atgttctttg gcgttgccga acctgtaatg cattatcttt
      481 cgccacccgt tggcactcca gaaaccgttg cggcagctaa ggaagcaatg cgtctgacct
      541 ttttccactg gggactgcac gcatgggcaa tttatgccat tgtggcgctg attctggcgt
      601 tcttcagtta ccgtcacggt ctgcctttaa ctctgcgctc cgcactctat cccattattg
      661 gcgatcgcat atacggacct gtaggacatg cggttgatat tttcgctgtt ataggcacgg
      721 tctttggcgt tgcgacatca ctgggttacg gtgttttgca ggtgaatgcc ggtttgaacc
      781 atcttttcgg ggtgcccatc aatgaaacgg tgcaggtaat tctgatcgtg gtcatcacgg
      841 ggttagcgac gatttcagtg gtgtccggtc tggataaggg aatacgtatc ctgtctgaac
      901 tcaatctggg tctggctttg ttgctcctgg cgctggtcct gtgtctggga ccaaccgtgc
      961 ttctgctgaa gtcatttgtg gaaaatacgg gcggttatct ttcggaactg gtgagtaaaa
     1021 cgttcaacct ttacgcgtat gagcccaagt cgagcaactg gctggggggc tggacattac
     1081 tgtactgggg atggtggctt tcatggtcgc cgtttgtggg gatgttcatc gcacgggtct
     1141 cccgcgggcg aaccattcgc gagtttgtca ccggcgtgct gtttgttccc gcgggtttta
     1201 cgctaatgtg gatgacggtg tttggtaaca gcgcgatcta tctcattatg aaccaggggg
     1261 ccacagacct cgccaatact gttcagcagg atgtggcgct ggccctgttt aatttcctgg
     1321 agcatttccc gttctcttct gtgctgtcat tcattgcaat ggcgatggtc atcgtcttct
     1381 ttgtaacgtc tgctgattcg ggggcaatgg ttgtggatac tctggcatca ggtggagtgg
     1441 caaacacacc cgtctggcag cgaatattct gggcctcgct catgggcatt gttgcaattg
     1501 cgcttctcct tgccggaggg ctaagtgcgc tgcaaacggt gacaatagcg agtgcattgc
     1561 ccttctcagt gatcttacta atatctatat acggactttt aaaagctttg cgccgggatt
     1621 tgaccaagcg tgaaagcctg agcatggcga caattgctcc tacggctgca cgtaacccaa
     1681 ttccttggca gagaaggtta cgcaatatcg cgtatctgcc gaagcgatct cttgtgaaac
     1741 gttttatgga cgacgttatc cagcctgcca tgacgctggt tcaggaggaa ctgaacaagc
     1801 aggggacgat aagccacatt agtgatgcag tcgacgatcg tattcgtctt gaagtcgatt
     1861 tgggcaacga gctgaatttt atatatgaag tgaggcttcg cgggtatatc tcaccgacct
     1921 tcgcgctcgc cgcaatggat aatgatgagc agcagactga acaacatcga tattatcgcg
     1981 ctgaggttta tctcaaagaa ggcggtcaaa attatgatgt gatgggctgg aaccaggaac
     2041 agctgattaa tgacatactg gaccagtacg aaaaacacct gcacttcctg cacctggttc
     2101 gttaatagca acatgccatc ctgggggcgg caattattat cccggccgca atatgaggga
     2161 atgcagaatg atttcacgct ggaaatggat gctgaagcag acaattaaaa aactatggtt
     2221 cagggcaacg ttattcgcaa ttgtcgcgat aataacggcc cttttatcaa ttctttttaa
     2281 atcaatgata cctgagtcgg tttccgtgaa ggttggtgcg gaagcagtcg ataacattct
     2341 gaacatactg gcatcgagta tgctggcagt gaccacattt tcgctgagta tcatggtcac
     2401 agcctacggt tcagccacta ctaatgtgac tcccagagct acgcgtttag ttgttgaaga
     2461 cgtcaccaca caaaatgtac tggccacctt catcggttct tttctcttca gtctggtagg
     2521 gattattgcc ctcaatatgg gagcttatgg agaaaggggg agagtcattt tattcattgt
     2581 cacactggtt gtcattgcct taatcctcat cacattgctt cgctggatac agcatttgac
     2641 ctctctgggg agggttggtg agacaacggc acaagtagaa caggcggcca tcgaaacatt
     2701 tattgcgaga gcaagaaatc cctgtctcgg tggatatcca tggcttgaga acaatgaaca
     2761 gccgaaagga acggttgcag tttatccgaa gaagattggc tatgttgaat atattgatat
     2821 ggtgaaactt agcaagctgc tgaccaatga tccccgtcat gtatacctcg tggcgcagcc
     2881 aggcagtttc atacatccgt ccatgccagt tttgtacctg agtcagggcc aggagtcgtc
     2941 aatcaacacc gatttacttg agacgattat tgtctcggat gcacgttcat ttgctcagga
     3001 tcctcgattt tgtcttagcg tcatggccga aatagcctgc cgggcccttt cccctgcagt
     3061 gaacgatcct ggaaccgcca ttgatgtcat tggcagaggt gttcgtatac tttccactta
     3121 cgcgcagaat aaatctgatg aaatagaagt gaaatatcct tcagtacatg ttgcaccact
     3181 tcagaataac gatctactgg aagacttttt ctcacctgtc gcgcgcgatg gtgccagtat
     3241 gagggagatt cagataagag tccttaaagg actgtcgatg ctgagtaaag gttggcctga
     3301 gatatttgct gaagccgcac ataccctggc atttgaaaca ttagagcatg caactcgtgc
     3361 tgaccatata gattctgata gatatctaat aaaatcaact tattataatt tatttagtgg
     3421 cgaatattct aataaaaaaa catagctgca gagcaagcaa ttcggttgtt ggcaagtcaa
     3481 gggcaaagca ttcgcgtgag aatgggcgta tgctttgtgc caaaagcaaa cgttgctaat
     3541 tctgcactgt tcagcgatgg cgcagtagag acaggcactg ccactgtcca gtcggcaaaa
     3601 gcgaacactt tgccactgcg gggataaact gattgaagct ctcgcgcaaa atcacttatt
     3661 cttttgcgtt aataaaatgt aggagatggg t
//
LOCUS       DAZPCS010000246         3548 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZPCS010000246 DAZPCS010000000
VERSION     DAZPCS010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.252"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..465
                     /gene="pgrR"
                     /locus_tag="ACHUQ7_003884"
     CDS             <1..465
                     /gene="pgrR"
                     /locus_tag="ACHUQ7_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFR1137063.1"
                     /translation="RLGGEMDKDMIAIRIGPDIPMAIVGSPDYFSRRSAPTSVSQLID
                     HQAINLYLPTSGTANRWRLIRGGREVRVRMEGQLLLNTIDLIIDAAIDGHGLAYLPYD
                     QVERAIKEKKLIRVLDKFTPDLPGYHLYYPHRRHAGSAFSLFIDRLKYKGAV"
     gene            802..2415
                     /gene="mppA"
                     /locus_tag="ACHUQ7_003885"
     CDS             802..2415
                     /gene="mppA"
                     /locus_tag="ACHUQ7_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFR1137064.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSMHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(2466..3497)
                     /gene="ynaI"
                     /locus_tag="ACHUQ7_003886"
     CDS             complement(2466..3497)
                     /gene="ynaI"
                     /locus_tag="ACHUQ7_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFR1137065.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSISNYVIKNYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
BASE COUNT         1006 a          772 c          819 g          951 t
ORIGIN      
        1 cgtctgggtg gggagatgga taaagatatg atcgccattc gaatcgggcc agatatacca
       61 atggctattg ttggctcacc ggattatttt tctcgtcgaa gtgctccaac gtcagtgtca
      121 caattaatag atcatcaggc aattaatttg tatcttccca catcgggtac agcaaatcgc
      181 tggagattaa tacgcggtgg acgtgaagtt cgtgttcgca tggaaggtca gcttttactg
      241 aatacgatag acctgatcat tgatgctgca attgatgggc atggattggc gtatctacct
      301 tatgatcagg ttgagcgggc tattaaagaa aaaaaactga tacgtgtttt ggataaattc
      361 actccagatt tacccggtta tcacctgtac tatccacacc gtcgacatgc tggctcggca
      421 ttctcattat ttatagatag gctgaagtat aaaggtgctg tttagcacta cttgctgata
      481 cattaattta attcttctct taacgtattc tcagttcctt tcaacgtttt ggttattttt
      541 tattcttcgt acaatggcga cagatgctga ttatgatacc gaaaactggt ttgaacgtgc
      601 gaagcccgaa cgtagtgttc ggtgttctat gtgctttgcc acattttggc gtctattatt
      661 tacactaaat ctgatttgat atattgatac ttaaaacatt tgatgcttcc tttgtcactt
      721 ttttgatgga agttgtttgc atttctttaa ggcgaaacaa ataattacgc atcaatttta
      781 atgtcggtta gagggaaact tatgaagcac tctgtttcag ttacgtgttg tgcgctgttg
      841 gtcagcagca tttctctttc gtatgctgca gaagttccga gcggcacagt actggcagag
      901 aagcaggagc tggtgcgcca cattaaagat gagcctgcgt cgctggatcc cgctaaagcc
      961 gtgggcctgc cagagattca ggtcattcgc gatctgtttg aaggtctggt gaatcagaac
     1021 gaaaaagggg agattgtccc cggcgtcgcg actcaatgga aaagtaatga caaccgtatc
     1081 tggactttta ccctgcgcga taacgcaaaa tgggcggatg gcacaccggt aacggcgcaa
     1141 gattttgtct acagctggca acgtctggtg gacccaaaaa cattgtcgcc atttgcatgg
     1201 tttgccgcgc tggcgggaat caacaacgca caggcgatta ttgatggtaa agctacgcct
     1261 gatcagcttg gcgtcaccgc agttgatgcc catactttga aaattcagct tgataaaccg
     1321 ttgccgtggt ttgtgaattt aaccgctaac tttgccttct tcccggtgca aaaagccaac
     1381 gtagaaagcg gtaaagagtg gacgaaaccc ggaaatctga tcggcaatgg cgcttatgtt
     1441 cttaaagagc gcgtagtcaa tgaaaaactg gtcgtggtac cgaataccca ttattgggat
     1501 aacgccaaaa cggtgctgca aaaagtgacc ttcctgccaa ttaatcagga atccgcagcc
     1561 actaagcgtt acctcgcggg ggatattgat atcaccgaat ccttcccgaa aaatatgtat
     1621 cagaagctgt tgaaggatat tccggggcag gtttatacgc caccgcagct cgggacctat
     1681 tattatgcct ttaacacgca aaaagggccg acggcagatc agcgcgttcg cctggcatta
     1741 agtatgacga tagatcgccg cctgatgacc gaaaaagtgt tggggacggg cgaaaagcca
     1801 gcgtggcatt ttataccaga tgttaccgcc ggatttacgc cggaaccttc gccgtttgaa
     1861 caaatgagtc aggaagaact gaatgcgcag gcaaaaactt tgttgagcgc agctggttat
     1921 ggtccgcaaa aaccgctgaa gctgacgctt ttgtataaca cttcagaaaa ccatcaaaaa
     1981 attgcgattg ctgtagcatc gatgtggaaa aagaaccttg gcgtagatgt taaattgcaa
     2041 aatcaggaat ggaaaaccta tatcgatagc cgtaacaccg gcaattttga tgttatccgc
     2101 gcctcatggg tgggggatta taatgaaccc tccactttcc tgacattatt aacgtcaatg
     2161 cattcaggaa atatttcacg ctttaacaat ccggcatacg acaaagttct ggcccaggca
     2221 tcgacggaaa ataccgttaa agcgcgtaat gccgattaca acgcggcaga aaaaatcctc
     2281 atggagcaag caccgattgc accaatttat caatatacca atggacgatt aatcaaaccg
     2341 tggctgaaag gttatcccat taataaccct gaagatgtag cgtacagtcg gactatgtat
     2401 attgtgaagc attgatgtga tgggaactgg cgttaccctt gtggataacg ccagtgatgt
     2461 ctgatttagc gcccctgttc cggcggtgta atgttatcca tatacagcgt ctggctgggg
     2521 aaggcaaagt ccgcgccgtg tgactgtaca atatcgataa tcttcaaata aacgtcttgc
     2581 tgtgcagcaa gccactcagc ccataccgtg gttttggtaa agcaataaac cataatattc
     2641 aatgacgagt cagcaaactg gttgaaataa accagtaagg tttgtcgctg gtcgatggcc
     2701 gggtgatttt tcagcatctc acgtacggct tcgacaataa cgcccacttt tgccgcatcc
     2761 tcataacgta aaccaatggt cgtggtaatg cggcggttgg tcattcgtcc tgggttttct
     2821 acgctgatcg acgaaaacag cgagttcggt acgtacaatg gacgattatc aaaggtcgta
     2881 attttggtaa ttcgccagcc aatttccgct actgtacctt cgatatttct gtccggtgaa
     2941 cggatccagt cgccaatact gaaaggacgg tcgaaataga gcatgatccc ggaaaagaaa
     3001 ttactcagaa tatctttacc ggccataccg acagccagac caccaatacc accaaaggtc
     3061 agcaagccag aaaggctcat gccgaaatgt tcgccataaa gcagaacaag cactacaata
     3121 atggtgattt tgataatgcg cgacataatc cgcgcactgg tgatatcgcg acctttttta
     3181 atctgctgtt tttcaaactg attaatcagc agaaatagct taatcgtcag aataaccgca
     3241 atcagggacg tacagataaa atcgataacg cctggggtga taaatttgag tttatagttt
     3301 tttataacat aattactgat gctaccaaca gcactgataa ttatggtgta gattaaaaat
     3361 tgcaccgcat ggaataaaaa tccttttctt ttacgatttc cacggcgaaa ccaaaagctc
     3421 atcagaatca atgctgcgca gctaccgaaa ataatgacca gattaagcgc attatttgta
     3481 aacagttcag cgatcattgt tttatcaggc tcctccagat aattgtcgtc atgccggaaa
     3541 cccctggc
//
LOCUS       DAZPCS010000247         3497 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZPCS010000247 DAZPCS010000000
VERSION     DAZPCS010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3497
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.253"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..311
                     /locus_tag="ACHUQ7_003887"
     CDS             <1..311
                     /locus_tag="ACHUQ7_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Involved in anaerobic NO protection; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HFR1137066.1"
                     /translation="LDNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQ
                     YYNQLHQLPLVALPLPFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA
                     "
     gene            468..1217
                     /locus_tag="ACHUQ7_003888"
     CDS             468..1217
                     /locus_tag="ACHUQ7_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFR1137067.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            1239..1805
                     /gene="yieF"
                     /locus_tag="ACHUQ7_003889"
                     /gene_synonym="chrR"
     CDS             1239..1805
                     /gene="yieF"
                     /locus_tag="ACHUQ7_003889"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFR1137068.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(1859..3196)
                     /gene="adeP"
                     /locus_tag="ACHUQ7_003890"
     CDS             complement(1859..3196)
                     /gene="adeP"
                     /locus_tag="ACHUQ7_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFR1137069.1"
                     /translation="MSHQHTTQTSGQRMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPDYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            3361..>3497
                     /locus_tag="ACHUQ7_003891"
     CDS             3361..>3497
                     /locus_tag="ACHUQ7_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709482.1"
                     /note="YieH; catalyzes the dephosphorylation of
                     phosphoenolpyruvate, AMP and p-nitrophenyl phosphate;
                     purine and pyrimidine nucleotides are secondary
                     substrates; member of the haloacid dehalogenase-like
                     hydrolases superfamily; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFR1137070.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     R"
BASE COUNT          961 a          908 c          864 g          764 t
ORIGIN      
        1 cactggacaa tgtgttacag gagctggggc gcgaacgcac gattgctatg agcctgccgg
       61 aattcgagca gtcactgttt atggcagcgc aacccgacaa tctgctactg gcgaccgcgc
      121 cgcgctactg tcagtactac aatcaactcc atcaactgcc gttggttgct cttcctctcc
      181 cgtttgacga aagccagcaa aaaaagctgg aagttccttt taccctgctg tggcataaac
      241 ggaacagcca taatccgaag atcgtctggt tacgggaaac cattaaaaac ctttacgcgt
      301 cgatggcata accgaatcgt atgaaacgcg acccaatttc acaataaaat gtaaaaaagt
      361 tgtaataagc ttgtctgaat cgaactttta gccgctttag tctgtccatc attccagtaa
      421 atgattactc ttgtattcat aatggaccat taagcatgga gcgaaaaatg gcgactcact
      481 ttgcccgagg gattttaacg gaaggacatc tgatttctgt tcgtctcccc tcccagtgtc
      541 atcaagaagc ccgaaacatt ccccctcatc gtcaaagccg ttttctggcg tccagaggtt
      601 tactcgcaga actgatgttc atgctgtatg gcattggcga attgccggaa atcgtcaccc
      661 tgccgaaagg taaaccggtt ttcagtgata aaaatttgcc ttcgttttct atttcctatg
      721 ccgggaatat ggttggcgtg gcgttaacaa ccgaaggtga atgtggcctc gatatggaac
      781 tacagcgtgc gacgcgcggg tttcatagcc cacacgcgcc cgataaccac accttttcca
      841 gcaatgaatc gctatggatc agtaaacaaa acgatcctaa cgaagcgcgg gcgcagctca
      901 ttacgctgcg ccgaagcgtg ctaaaactaa ccggtgatgt tttgaatgac gatccgcgcg
      961 atctgcagct gctgcccatt gccggacgcc tgaaatgtgc tcatgtaaat catgtagaag
     1021 cgttatgcga cgcggaggac gtgctggtgt ggtccgtggc ggtcacgccc gcgattgaaa
     1081 agctcagtgt ctgggagtta gatggcaaac acagctggaa aagcctgccg gatattcaca
     1141 gccgcgccaa caatcctacc agccggatga tgcgttttgc ccagctctct accgtgaagt
     1201 ctttttcgcc aaattgatag acaaccacag gagtcattat gtctgaaaaa ttgcaggtgg
     1261 ttacgttact ggggagcctg cgcaaaggct catttaatgg catggttgca cgtaccctgc
     1321 cgaaaattgc tccggcgagc atggaagtca atgcgttacc ttccattgcc gacattcccc
     1381 tgtatgacgc tgacgtacag caggaagaag gttttccagc aacggttgaa gctctggcgg
     1441 aacagatccg tcaggctgac ggtgtggtga tcgtcacgcc ggaatataac tactcggtac
     1501 cgggtgggct gaaaaatgcc atcgactggc tttcccgcct gccggatcaa ccgctggccg
     1561 gtaaaccggt gttgattcag accagctcaa tgggcgtgat tggcggcgcg cgctgtcagt
     1621 atcacctgcg ccagattctg gttttcctcg atgcaatggt gatgaacaag ccggaattta
     1681 tgggcggcgt gattcagaac aaagttgatc cgcaaaccgg agaagtgatt gatcagggta
     1741 cgctggacca cctgaccggg caattgaccg catttggtga gtttattcag cgagttaaga
     1801 tctaaataaa aaacccgcca gtaatcatgc atggcgggtt tttaatgcgc tatcgatttt
     1861 aatgagcgtc gataaataca atcttcagga taaacagcag cgcaacgatg attacgcacg
     1921 ggctaaggtc acgcagacgc ccggtaccaa tcttcatcac gcagtaggag ataaagccca
     1981 gcgcaatacc ttcggtaatc gaaaagctga acggcatcat cacggcggta ataaacgccg
     2041 gaacagattc agtaagatcc tgccagttca cgcgagcaag acttgaggtc atcaacacgc
     2101 caacgtagat caacgcgcca gctgcagcgt agtctggcac cattcccgcc agcggcgaca
     2161 gaaagataac cagcaggaac agcagaccaa caaccactgc cgtcagaccg gtacgaccgc
     2221 caaccgatac gccggaagag gactcaatat aagccgtaac ggaagaagta ccgataaacg
     2281 aaccggtcac ggaagagata ctgtcgacat acagcgcctg cttcatgcgc gggaatttcc
     2341 ccttctcatc cgccagacct gctttatcgg tcacgccaat cagcgtaccg gaggagtcaa
     2401 acaagttgac caacatgaaa gagaaaatca cccctgccag cccgaggtta aacgacccgg
     2461 ctaaatctac atgacccaca actgtcatta cgctcggcgg cgcagaaacg atgccattgt
     2521 agtgcacatc acccagcatc cagcccagca gcgtcgtcac cacgatagaa accagcaccg
     2581 ctgcgtgaat gttgcgcgag gccagaatag caatgatgaa aaagccgagg atacccagaa
     2641 gtacgctgtg agaagtcaga ttaccgatgc tcaccagcgt ttccgagtta gcgacaatca
     2701 cacctgcgtt tttcagcccc atcatgccga taaacaggcc gataccgctg gtaataccca
     2761 cacgcagact taccggaatg ttagcaatca tccagtaacg aacgcggaaa atggtcaaaa
     2821 gaagcagtcc gacagcgccc cagaagattg cgcccatacc gatctgccac ggcagcccca
     2881 ttgcctggac aaccacaaaa gcgaaaaagg cgttaagccc catcgcagga gccagcgcca
     2941 ccggaaggtt tgcaaacaat cccatcataa tgctaccgaa agcagcaatc agacaggttg
     3001 ttacgaagac ggcactggtg tccatgccag caacgcccag aatttgtggg ttaacaaata
     3061 cgatgtagac catcgtcagg aaggtggtga acccggcaat cacttcagtt cgtgccgtag
     3121 taccatgttc gcgcagttta aaaacacgtt ccagcatcct ctgaccagaa gtctgggtgg
     3181 tatgttgatg actcattatc tatttccgaa caaggaggga aaatccgtcg ctatcgtata
     3241 ccaaaatgcg acaataggcg cgtttgtgag agactttttt attggattta cttatacggc
     3301 aacgattgcg ttgcgcaaat cggctctacg aaaacgttaa actgattaaa aaggaaaggc
     3361 atgtcccgga tagaagcggt atttttcgac tgcgacggta cgctggtcga cagtgaagtc
     3421 atttgctccc gcgcatatgt aacgatgttt caggaatttg gtattacgct cgatcctgaa
     3481 gaggtattca aacgttt
//
LOCUS       DAZPCS010000248         3456 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZPCS010000248 DAZPCS010000000
VERSION     DAZPCS010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.254"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(226..429)
                     /locus_tag="ACHUQ7_003892"
     CDS             complement(226..429)
                     /locus_tag="ACHUQ7_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1137071.1"
                     /translation="MNETELKHVIALLLEDAKRLQQLEPNAGTGARIWLAKEALESGD
                     YDSEEAFYKAEGRAGYSPGLGGV"
     gene            complement(431..586)
                     /locus_tag="ACHUQ7_003893"
     CDS             complement(431..586)
                     /locus_tag="ACHUQ7_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFR1137072.1"
                     /translation="MNTIDLGNNESPVYGVFPNQDGTFTAITYTKSKTFKTEAGARRW
                     LGRHSGE"
     gene            complement(789..1196)
                     /locus_tag="ACHUQ7_003894"
     CDS             complement(789..1196)
                     /locus_tag="ACHUQ7_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1137073.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            1273..1500
                     /locus_tag="ACHUQ7_003895"
     CDS             1273..1500
                     /locus_tag="ACHUQ7_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFR1137074.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPGKVIHENQA"
     gene            1484..1906
                     /locus_tag="ACHUQ7_003896"
     CDS             1484..1906
                     /locus_tag="ACHUQ7_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFR1137075.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELSLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSPGDSVVVH"
     gene            1984..2761
                     /locus_tag="ACHUQ7_003897"
                     /pseudo
     CDS             1984..2761
                     /locus_tag="ACHUQ7_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2768..3456
                     /locus_tag="ACHUQ7_003898"
                     /pseudo
     CDS             2768..3456
                     /locus_tag="ACHUQ7_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309972.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          885 a          764 c          940 g          867 t
ORIGIN      
        1 ccagaggatt acgaagggct tcataaatat atgctctcac taggattcag gagaacgatc
       61 ccccatggag atggttcgta taatcaactc ccggacggaa cttatgtttc cgaaaagggc
      121 ggtgatattt atcaaattcg cagccagata tctgactatg cagaccgact atccgggtat
      181 cgcgcgtctg tttttgtttg cgaattcagt caatgcgcat ggtatttata ccccgccaag
      241 acccggtgaa tatcctgcac ggccttctgc tttgtagaag gcttcttcgc tatcataatc
      301 gccagattcc agcgcttctt tagccagcca gatgcgtgcc ccagtgcctg catttggctc
      361 cagttgctgg aggcgttttg catcttctag gagtagagcg ataacgtgtt ttaattctgt
      421 ctcgttcatt ttactcacct gaatgtcttc ccaaccaacg acgtgcgcca gcttcggttt
      481 taaacgtttt gcttttggta tacgttatgg cggtgaatgt gccgtcctgg ttggggaaca
      541 cgccgtacac cggagattcg ttgttgccaa gatcgatagt attcatgttg accccatttc
      601 cccttaacgc cggggtagcg gaactgtttg ctgagaacac cgtgcggtgt cttgatgaat
      661 aaaatttaga ataacctaag gagggtgatc aagatttttg tgtagaaaaa cctaagtttt
      721 ttgatgtaaa aaacacaagt gtttgaaagt ttgtgctttt tattacaggg tgtggagaaa
      781 aaaggggatt atttgtttgc gcttcttttg cgagctttga gtagttcttc aaaaagtttg
      841 ttgaaatttt caactcgagc acgcatctct gacaacaggg ccttttgctc tgactcaggc
      901 agtgcgtcga acagttgaag taactctttt tgatcttctg tcagaatggc tggctgatta
      961 tccgggatcg gttcgcctgg ttgtttatct tcatccccaa aaagaagcca agtcggtgag
     1021 cactgaagcg cttggctcag tgcgaataat cttttccccg ccggctgtgt ttcatctctt
     1081 tcccattgag aaattgttac gtgagccact ttgaccagct tacctaatgc ggcctgagac
     1141 agttttaatt ttttacgcct atgtaagagg cgagcaccga aggtttcgtt tttcatatta
     1201 gggaattcta atttttcttg acttaggttt ctctacggcc tggtttcctt aggaaaatct
     1261 aaggagctcg atatgttgaa aattgatgct atagcgtttt ttggcagcaa aacaaagctt
     1321 gccaatgccg caggagttag gctggcaagc attgctgcat ggggggaact ggttcctgaa
     1381 ggtcgcgcga tgcgcctgca agaggcatct ggcggggaac ttcagtacga ccccaaagtt
     1441 tatgacgaat atcgtaaggc aaaacgacct gggaaggtga ttcatgaaaa tcaggcatga
     1501 gcacatcgaa tcagtgctgt tagccctggc agccgaaaaa gggcaggcgt gggtcgctaa
     1561 cgcaattact gaagaatatc tgcggcaggg gggcggcgaa ttgtctctgg taccaggcaa
     1621 ggactggaac aatcagcaga atatctatca ccgttggttg aaaggtgaaa cgaaagcgca
     1681 aagggaaaaa attcagaaac tgatccctgc ggttctggca attcttccgc gtgagctgcg
     1741 tcaccgactc tgtatcttcg ataccctgga acgccgtgca ttactggcgg cgcaggaagc
     1801 gttgagtacg gcaattgatg cgcatgatga tgctgtccag gccgtttacc ggaaagcaca
     1861 tttcagcggt ggtgggtcgc ccggcgattc tgtcgtagtg cattgattga aattaatcgt
     1921 gccggattgt tttgttcggt atcagttaaa tgtaacgctg cgagcgttac aaggtgaaaa
     1981 caaatggctt caaactggat aaagctcgag gttattacgc cggataagcc ggaaatattc
     2041 aggcttgctg agattctgaa tattgatcca gatgccgcat tagggaaggt tattcgcttc
     2101 tgggcatggg cggatcaaca aatgatagac ggtaatgcag attgtaacgc tcgcggcgtt
     2161 acaaaaagtg caatagatcg catcactttt atgtctggtt ttgctgatgc gttaattcag
     2221 gttggatggc tggtcgaaaa tgacggtggg ctttctctac ctaactttga acgtcataac
     2281 ggaaaaagct ctaaaaaacg ggcggttaca aacgagcgag taacaaaaat acgcgaactg
     2341 aaacgaaaag gtaacgctgc cagcgttaca caaacggatc aaaaagcgtt accagtggaa
     2401 gaggaagagg aagatctaaa tactgatctc cccctaaatc cccctcgcca aaaacgagcg
     2461 tctaaaaaat tcgagccgga ggctattgag ctgcccgatt ggttgccgga aacactctgg
     2521 catgagtggg tccggttcag acaggcattg cgaaaaccga ttcgaacgga gcagggcgat
     2581 acgggaactg gaaaaattcc gtcagcaggg ttttacacct gagcaggtga ttcgacacag
     2641 catcgccaat gaataccagg gcctgttcgc gccgaaaggt gttcggcctg agacgttgct
     2701 ccgacaggtt aacaccgtct cgttgccgga cagtgcgatc ccgccaggct tcagggggta
     2761 acggaccatg aaaaatattg cgacaggcgg cgttctggaa cgcatccgcc gactgacccc
     2821 gcaacatgta accgccccat tcagaacggt agcggagtgg cgcgagtggc aacttgctga
     2881 aggccagaaa cgtagcgagg agatcaaccg cctgaatcgc cagttgcggg tggaaaaaat
     2941 tctgaatcgc tcaggcatcc agccgttgca ccgtaaatgc tcgtttgcga attaccaggt
     3001 gcagaacgac ggccagcgat acgcgttaag ccaggcgaaa tccatcgccg atgaactgat
     3061 gaccgggtgt acaaattttg cgttcagcgg aaaacctgat accgggtaga atcacttagc
     3121 ggcagctatc gggaatcgcc tgctgaaaga cggtcagaca gtgattgtgg ttaccgtggc
     3181 tgatgttatg agcgccctgc acgccagcta tgacgacggg cagtcaggcg aaaaattttt
     3241 gcgggagctg tgcgaagtgg atctgctggt tcttgatgaa attggcattc agcgcgagac
     3301 gaaaaacgag caggtggtgc tgcaccagat tgttgatcgc cggacagcgt cgatgcgcag
     3361 cgtggggatg ctgacaaacc tgaactatga ggtgatgaaa acattgctcg gcgagcgggt
     3421 gatggatcgc atggtcatga acggcgggcg ctgggt
//
LOCUS       DAZPCS010000249         3415 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZPCS010000249 DAZPCS010000000
VERSION     DAZPCS010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3415
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.255"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..239
                     /locus_tag="ACHUQ7_003899"
     CDS             <1..239
                     /locus_tag="ACHUQ7_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HFR1137076.1"
                     /translation="LLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMP
                     ALSEKIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            249..887
                     /locus_tag="ACHUQ7_003900"
     CDS             249..887
                     /locus_tag="ACHUQ7_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFR1137077.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            890..2053
                     /locus_tag="ACHUQ7_003901"
     CDS             890..2053
                     /locus_tag="ACHUQ7_003901"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFR1137078.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSRELFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            2137..>3415
                     /locus_tag="ACHUQ7_003902"
     CDS             2137..>3415
                     /locus_tag="ACHUQ7_003902"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFR1137079.1"
                     /translation="MHWQTHTIFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPVTMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEG"
BASE COUNT          760 a          804 c          987 g          864 t
ORIGIN      
        1 cattgttagg ttacgttatc ccattatcca gtgcggcaat caatgcggtg agtattccag
       61 actatttcgc ctggggcggg attgcactgg tgattcagtt actcgttttt gctggtgtca
      121 gactttatat gcccgcatta agcgaaaaaa ttattaatca caataccgca gcaggaatgt
      181 ttatgggaac cgccgcgctg gctggcggta tttttaacgc agcttgtatg acatggtaat
      241 ggacgatcat ggccagaaaa cgcaaatcaa gaaataacag taaaattggt cacggagcga
      301 tcagtcgcat tggtagaccg aataatcctt ttgaaccgcg tcgcaatcgc tacgcacaaa
      361 aatacttaac gttggcgcta atgggcggtg ccgctttttt cgtattgaaa ggttgtagcg
      421 atagtagcga tgtcgataac gacggcgacg gaacatttta cgcgacagtg caggattgta
      481 ttgatgacgg taataatgcc gacatttgcg cgcgtggctg gaacaacgcc aaagcggcat
      541 tttatgccga tgttccaaag aatatgactc agcagaactg tcagtctaag tacgaaaatt
      601 gctactacga caatgttgaa cagagttgga tcccggtcgt ttctggattt ttgttaagcc
      661 gggttattcg taaagatcgc gatgagccgt tcgtctataa cagcggcgga tcctcatttg
      721 cttcccgccc cgtctggcgc aacacttccg gtgattactc ctggcgcttc ggttctggca
      781 aaaaagagtc ttactcttca ggcggcttta ccaccagaaa agcgtcgacc gtttcgcgcg
      841 gcggctatgg tcgttcttcc agcgcccgtg ggcattgggg aggctaatca tgctgagaca
      901 caacgttcct gtgcggcggg atctggacca gatcgccgct aacaacggtt tcgactttca
      961 tatcatcgac aatgaaatct attgggacga gagtcgggct taccgtttta ctctgcgcca
     1021 gattgaagag cagatcgaaa aaccgactgc ggaactgcat cagatgtgcc ttgaggtggt
     1081 ggatcgcgcg gttaaagacg aagagatcct gacgcaactg gcgatcccgc cgttgtactg
     1141 ggatgtgatc gccgaaagct ggcgcgcacg cgatccttcg ctgtatggcc ggatggattt
     1201 tgcctggtgt ggcaatgcgc cggtgaagct gctggagtac aacgccgata cgccgacctc
     1261 gttgtatgag tcggcttatt tccagtggtt gtggctggag gatgcccggc gcagcggcat
     1321 tattccgcgt gatgccgatc agtacaacgc cattcaggaa cgcctgattt cgcgctttag
     1381 tgagctttac agtcgggaac tgttttattt ttgctgctgt caggacaccg atgaagacag
     1441 gagtaccgtg ctgtacttgc aggactgcgc ccagcaggca gggcaggagt cgcggtttat
     1501 ctacattgaa gatctcggtt tgggcgtcgg cggcgtactg accgatcttg atgataatgt
     1561 catccagcgt gcatttaagc tgtatccgct ggagtggatg atgcgtgacg ataacggtcc
     1621 gctgctgcgc aagcgtcgcg agcaatgggt ggagccgtta tggaaaagta tcttgagtaa
     1681 taaagggcta atgccgctgc tttggcgctt cttccctggt catcctaacc ttcttgcatc
     1741 ctggtttgag ggtgaaaaat cgcagattgc cgctggcgaa agctatgtgc gtaaaccgat
     1801 ctactcgcgc gaaggcggaa acgtcaccat tttcgacggt cagaataacg tcgttgacca
     1861 cgctgatggt gattacgccg atgaaccgat gatctaccag gcgtttcaac ctctgccgcg
     1921 atttggcgat agctacacac tcatcggtag ctggattgtc gatgatgaag cgtgcggaat
     1981 ggggatccgt gaagataaca cactgatcac caaagacacc tcacgtttcg ttccgcatta
     2041 cattgctgga taagaatgtt ttagcaatct ctttctgtca tgaatccatg gcagtgacca
     2101 tactaatggt gactgccatt gatggaggga gacacagtgc actggcaaac tcacaccatt
     2161 tttaatcaac ctataccatt aaataacagc aacttatacc tgtctgatgg cgcgctttgc
     2221 gaagcggtaa cgcgtgaagg tgctggctgg gatagcgatt ttctagccag tattggtcag
     2281 cagttaggaa cggctgaatc ccttgaactg gggcggctgg cgaatgtaaa cccgcctgaa
     2341 ctgttgcgct acgatgccca ggggcggcgt ctggacgatg ttcgtttcca tcccgcatgg
     2401 cacctgctga tgcaggcgtt atgtaccaat cgggtgcaca atcttgcctg ggaagaagac
     2461 gctcgctccg gcgcatttgt ggcgcgcgcg gcgcgtttta tgttacatgc gcaggttgag
     2521 gctgggtcgt tatgtccagt aacgatgacc tttgccgcca cgccattgtt gttacagatg
     2581 ttacccacgc cgtttcagga ctggaccacg ccgctgctga gcgatcgcta cgattctcac
     2641 ttattgccag gtgggcaaaa acgcggtttg ttgattggca tgggaatgac ggaaaagcag
     2701 ggcggttccg atgtcatgag caacaccacc cgtgcagagc gtttggaaga tggctcttat
     2761 cggctggtgg ggcataaatg gtttttctcg gtgccgcaaa gcgatgcgca tctggtgctg
     2821 gcgcagaccg cgggcggtct gtcctgcttt tttgtgccgc gctttttgcc tgacgggcaa
     2881 cgcaacgcga ttcgcctcga gcggctgaaa gataagctgg gtaatcgctc taacgccagt
     2941 tgcgaagtgg agtttcagga tgccattggc tggttattgg ggcaggaagg ggaaggaatt
     3001 cgtctgatcc tgaaaatggg ggggatgacg cgttttgatt gcgccctggg tagccatgcc
     3061 atgatgcgcc gtgcattttc gctggcgatt tatcatgcac atcaacgcca tgtttttggt
     3121 aatccattga tccaacagcc ccttatgcgt catgtcttaa gtcgcatggc acttcagctt
     3181 gaagggcaaa cggcgttgct gtttcgtctt gcgcgagcgt gggaccggcg tgccgatgcc
     3241 aaagaagccc tgtgggcgcg tttatttacg cctgcggcaa aatttgtgat ctgcaaacga
     3301 ggtatgccgt ttgtggccga agcgatggag gtgctgggcg gcattggtta ttgcgaggag
     3361 agcgagctgc cgcggcttta ccgggagatg ccggtaaaca gtatttggga aggtt
//
LOCUS       DAZPCS010000250         3413 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZPCS010000250 DAZPCS010000000
VERSION     DAZPCS010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3413
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.256"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            75..791
                     /gene="nanC"
                     /locus_tag="ACHUQ7_003903"
     CDS             75..791
                     /gene="nanC"
                     /locus_tag="ACHUQ7_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFR1137080.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            811..1917
                     /gene="nanM"
                     /locus_tag="ACHUQ7_003904"
     CDS             811..1917
                     /gene="nanM"
                     /locus_tag="ACHUQ7_003904"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFR1137081.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            1982..2962
                     /locus_tag="ACHUQ7_003905"
     CDS             1982..2962
                     /locus_tag="ACHUQ7_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFR1137082.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
     gene            3072..3277
                     /locus_tag="ACHUQ7_003906"
                     /pseudo
     CDS             3072..3277
                     /locus_tag="ACHUQ7_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168579.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
BASE COUNT          995 a          726 c          801 g          891 t
ORIGIN      
        1 attacacaac ataaatgcta attgtttatg cgggctttgt attgctttct gtatcctaca
       61 aatgagtgaa atttatgaaa aaggctaaaa tactttctgg cgtattatta ctgtgctttt
      121 cgtccccatt aatttctcag gctgcgacac tggacgtacg tggtggatat cgtagtggaa
      181 gccacgccta tgagactcga ctcaaagtca gtgagggatg gcaaaatgga tggtgggcaa
      241 gcatggaaag taatacctgg aataccattc atgataataa aaaggaaaat gccgcactca
      301 atgatgttca ggttgaagtt aattacgcaa ttaaacttga tgatcaatgg acggtgcgcc
      361 cgggaatgtt aacgcatttt agcagcaacg gcacacgcta cggaccctac gtaaaactgt
      421 cctgggacgc gacaaaagat cttaattttg gcattcgcta tcgttacgac tggaaagctt
      481 accgacaaca agacttatcc ggtgatatgt ctcgtgataa cgttcatcgt tgggatggat
      541 atgtcactta ccatattaat agtgatttca ccttcgcatg gcaaacgacg ctatacagca
      601 aacagaatga ttatcgctat gcaaaccaca agaaatgggc gacggaaaat gcctttgttc
      661 tacaatacca tatgacgccc gatattacgc catacataga atatgactac cttgaccgcc
      721 agggtgttta caacggcaga gataatttat cggaaaacag ttatcgcatt ggtgtgtcat
      781 ttaaactgta gtagacagga gacagtcaca atgaataaaa ctataatggc gcttgctatc
      841 atgatggctt catttgccgc aaacgcgtct gtattaccgg aaactcctgt gccatttaaa
      901 agtggtaccg gagcaattga taacgacact gtctacattg gtttaggtag cgcaggtaca
      961 gcatggtaca agctagatac acaggccaaa gataaaaaat ggactgcgtt agctgcattc
     1021 cctggtggac cgagagagca agcaacctct gcatttattg atggcaatct gtatgtgttt
     1081 ggcggcattg gcaaaaacag cgagggattg actcaggtat ttaatgacgt acacaaatac
     1141 aaccccaaaa ccaatagctg ggttaaattg atgtcgcacg cgccgatggg catggcgggt
     1201 catgtgactt ttgtacacaa cggcaaggct tatgttactg gcggtgttaa ccagaatatc
     1261 ttcaatggct attttgaaga tctcaacgag gctggaaaag attcaaccgc tatagataaa
     1321 atcaacgccc actattttga caaaaaagca gaagattatt tcttcaataa gtttctgttg
     1381 tcttttgatc cctcaacaca gcaatggagt tacgctggcg aatcgccctg gtacggaacg
     1441 gctggtgcgg cggttgtgaa taaaggtgat aaaacctggc ttattaatgg cgaagccaaa
     1501 ccaggattgc gaacggatgc cgtatttgaa cttgatttca ccggtaataa tttaaaatgg
     1561 aataagcttg atcccgtctc atcaccagat ggcgtcgctg gcggttttgc ggggataagc
     1621 aatgattctc ttatatttgc tggaggggcc ggattcaaag gttcacgaga aaattaccag
     1681 aacggtaaga actatgcgca tgaaggcctg aaaaaatcat atagcactga tattcatctt
     1741 tggcataacg ggaaatggga taaatcgggt gaattatcgc aaggtcgggc ctacggagta
     1801 tcattgccct ggaataatag tttattaatt attggcggtg aaactgcagg cggcaaagcg
     1861 gtgacggatt cagttttaat ttctgtgaag gataataaag tcacagtaca aaactaacgc
     1921 ttcagggccc cggtaaaggg gccctgctat caatttgtca tttgtattac gcgaaaatta
     1981 tatgaacgca ataatatcac ccgattatta ctatgttctt accgttgctg gtcagtctaa
     2041 tgccatggcg tatggcgaag gactgccatt accggacagg gaagatgcgc ctcattccag
     2101 aattaaacaa ttagcgagat ttgcgcatac gcatcccgga ggcccgccat gtcactttaa
     2161 cgacattatt ccactgactc actgcccaca cgatgttcag gatatgcagg gttatcacca
     2221 tcctctggca acgaatcatc aaacacagta cggcaccgtt ggccaggcac tgcatattgc
     2281 acggaaatta ctgcccttca ttcctgataa tgcagggatt cttatcgttc cgtgttgccg
     2341 tggcggatcg gcttttaccg cgggcagcga agggacatat tcagaacggc acggagccag
     2401 ccatgatgct tgtcgttggg gaacggatac tccgctgtac caggatttag tcagcagaac
     2461 gcgagccgca ctggcaaaaa atccgcagaa caaattcctc ggcgtatgct ggatgcaagg
     2521 cgaatttgac ttaatgacca gtgactacgc gtcacaccct caacacttta atcatatggt
     2581 tgaagccttt cgtagggatc taaaacaata ccattctcag cttaataata ttactgacgc
     2641 gccgtggttt tgcggcgata ccacctggta ctggaaagaa aatttcccgc attcgtatga
     2701 agctatttat ggcaattatc aaaataatgt tttagccaat attattttcg tcgacttcca
     2761 gcaacaaggt gaaagaggac tgacgaacgc gcctgatgaa gacccggacg atttaagcac
     2821 gggatattac ggttcagcgt accggtcacc ggagaactgg acgacggcac tgcgaagcag
     2881 tcatttcagc acggcagccc gtcgggggat tatttctgac agatttgtag aagcaatttt
     2941 gcagttttgg cgcgaaaggt gagcggggag tttattcatg ggggatgtcc ttgttcgttc
     3001 tcagagtaga caacaaaaaa caaatgagat caaggtaata tctcttcact tgcgggctcg
     3061 aacagcaggc aattgccaat ataataatct ggcacaaggg tgaaaaacgc gcaccgcata
     3121 aaccgttgtt attgcttaca gattagccgg atgccttaat agacatccgc gccttttcga
     3181 ttatggctcg gaaatctacg aacatctaca cagtttactg gaacgttttg ggccacagcg
     3241 ttcgcagcac cgtcctgata tgccgttctg gcgattataa gtcgacggat tctggcaaat
     3301 agacgatgat gatatgctta atgatgttca aagaaattag gccattatta tattattgct
     3361 tgcattttct ttcattccta aaagcacccg ccattaatcc caggcgcatt atg
//
LOCUS       DAZPCS010000251         3413 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZPCS010000251 DAZPCS010000000
VERSION     DAZPCS010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3413
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.257"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            339..1397
                     /gene="rfaQ"
                     /locus_tag="ACHUQ7_003907"
     CDS             339..1397
                     /gene="rfaQ"
                     /locus_tag="ACHUQ7_003907"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFR1137083.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            1394..2518
                     /locus_tag="ACHUQ7_003908"
     CDS             1394..2518
                     /locus_tag="ACHUQ7_003908"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFR1137084.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFEVIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGADRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            2511..3308
                     /gene="rfaP"
                     /locus_tag="ACHUQ7_003909"
     CDS             2511..3308
                     /gene="rfaP"
                     /locus_tag="ACHUQ7_003909"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFR1137085.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPGRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
BASE COUNT          901 a          741 c          829 g          942 t
ORIGIN      
        1 tttgacttaa atagcaattt acccgccaat tcttgttgaa catctatact ttttttgtat
       61 ttttatgcga ttcattgatt tctgctgtac gtgcaggaag gaatcgcaga gcaacagtga
      121 aagaatatgg ttgctaatga ttttatttga ttgatttctt tattgaaaat caacaatgtg
      181 cttaaaaatc attcgggtcg gattgccgcg aaagaagtga tacactagcg cgtcaatatc
      241 agtgcggtat cagtattcag gtagctgttg agcctggggc ggtagcgtgc tttttctgct
      301 tcaccttaat cggataatct caacaaaaag agttacttgt ggataagcca tttcgaagaa
      361 ttttgctcat taagatgcgt tttcatgggg atatgttatt aactactccc gtcattagtt
      421 ctctgaaaaa aaattaccct gacgcaaaaa tcgatgtgct gctttatcag gacaccatcc
      481 cgatcctgtc tgaaaatcca gagattaacg cgctctacgg cataaaaaat aaaaaagcaa
      541 aagcctcaga aaaaattgcc aacttttttc atctcatcaa ggtattacgt gccaataagt
      601 atgaccttat cgtcaatctt accgatcaat ggatggttgc tatactggtt cgcttattaa
      661 atgctcgtgt gaaaatttcc caggattatc atcatcggca gtctgctttt tggcgtaaaa
      721 gtttcaccca tttggtgccg ttgcagggtg gaaatgtggt ggaaagtaac ttatccgtgc
      781 tgacaccatt gggacttgat tcgttggtga agcagacaac catgagttac ccgcctgcaa
      841 gctggaaacg tatgcgtcgc gaacttgatc acgctggtgt tggacaaaat tatgtggtta
      901 tccaacctac ggcgcggcaa atcttcaaat gctgggacaa cgccaagttt tccgctgtga
      961 ttgatgcctt acatgctcgt ggttatgaag tcgttctgac gtccggccca gataaagacg
     1021 atctggcctg cgtcaatgaa attgcgcagg gatgccagac gccaccagta acggcgctgg
     1081 ctggaaaggt gaccttcccg gaacttggtg cgttaatcga tcatgcgcag ctgtttattg
     1141 gcgttgattc cgcaccggcg catattgccg ctgcagttaa tacgccgctg atatcgctgt
     1201 ttggcgcaac agatcatatt ttctggcgtc cctggtcaaa taacatgatt caattctggg
     1261 cgggagatta ccgggaaatg ccaacgcgcg atcagcgtga ccgaaatgag atgtatcttt
     1321 cggttattcc ggcggcagat gtcattgctg ctgtcgataa attactgccc tcctccacga
     1381 caggtacgtc gttatgatcg ttgctttttg tttatataaa tattttccct ttggcggttt
     1441 gcagcgcgat tttatgcgta ttgctcagac agtcgccgcc cgaggtcatc atgttcgggt
     1501 ttatacccag tcgtgggaag gcgaatgccc tgatgtattt gaagtgatca aagtgccggt
     1561 taaatcgcat accaatcacg ggcgcaatgc ggagtatttt gcctgggtgc aaaaacattt
     1621 acgcgaacat cccgtcgata aagtcgttgg attcaacaaa atgccggggc tggacgttta
     1681 ttatgccgct gatgtttgtt atgccgagaa agtagcgcag gaaaaaggct ttttctatcg
     1741 cctgacgtca cgttatcgcc attatgccgc ctttgagcgg gcaaccttcg aacagggcaa
     1801 gccgacacag ctgctgatgc tgacagataa gcaaatcgcc gatttccaga aacattatca
     1861 gactgaagcg gagcgttttc atattctgcc accggggatt tatcctgatc gtaaatatag
     1921 ccagcagcca gccaatagcc gtgaaatctt ccgtaagaag aatggaataa ccgaacaaca
     1981 atatttattg ttgcaggtcg gttcagactt cacgcgtaaa ggtgccgatc gttccattga
     2041 agcacttgct tcgttaccgg attcgctgcg ccacaacaca ttgctatatg ttgttgggca
     2101 ggataaaccg cgaaaatttg aggcactggc agaaaaacgc ggcgtgcgca gtaatgttca
     2161 cttcttctcg gggcgcaacg atgtctcgga attaatggcg gcggcggatt tattactgca
     2221 tcctgcctac caggaagcgg cgggaattgt gctgctggaa gcgattactg caggattacc
     2281 ggtactaaca actgccgttt gtggctatgc gcattatatt gtcgacgcta attgcggcga
     2341 ggctattgct gagccattcc gccaggaaac attgaatgag attttacgca aagcgttaac
     2401 gcaatcttca ttgcgccagg cttgggcgga aaatgcgcga cattatgctg atacacaaga
     2461 tttatacagt ctgccagaga aagcggcgga tatcataacg ggtggtctgg atggttgaac
     2521 ttaaagagcc gtttgccacg ttatggcgcg gcaaagatcc ttttgaggaa gttaaaacct
     2581 tgcagggtga ggtatttcgt gaactggaaa ctcgccgtac tctgcgcttt gaaatggcgg
     2641 gaaaaagcta ttttctcaaa tggcatcgcg gcacgaccct gaaagagata atcaaaaatt
     2701 tactctcatt gcggatgcca gtattaggcg cagaccgcga atggaatgcg attcatcgac
     2761 tgcgggatgt cggcgttgat actatgtatg gggtggcatt cggcgaaaaa ggcatgaatc
     2821 cgctgaccag aacctcgttt attattaccg aagatctgac accaaccatc agtctggaag
     2881 attactgtgc tgactgggcg accaacccac cagatgttcg cgtaaagcgt atgcttatta
     2941 agcgtgtcgc gacgatggtg cgcgatatgc atgctgcggg cattaaccat cgtgactgtt
     3001 atatctgtca tttcctgctg cacttgcctt tttccggtaa ggaagaggag ttaaaaattt
     3061 cggtaattga cctacaccgg gcgcagcttc gcacgcgcgt tccaggtcgt tggcgcgata
     3121 aagatcttat agggctttat ttttcttcaa tgaatattgg cctgactcag cgggatatct
     3181 ggcgctttat gaaggtgtat tttgccgccc cgcttaaaga cattctcaag caggaacaag
     3241 gactgctgtc gcaagcagaa gcaaaagcca caaaaatcag ggaaagaacg attcgaaaat
     3301 cgttgtaacc cgtaacttat ttttgccaaa attttggata cagaataaat atgtctcaac
     3361 tcaatgatag tgacatcatc ctttttgagt ataattttca ttatcaaaat ata
//
LOCUS       DAZPCS010000252         3385 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZPCS010000252 DAZPCS010000000
VERSION     DAZPCS010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3385
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.258"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..171)
                     /locus_tag="ACHUQ7_003910"
     CDS             complement(<1..171)
                     /locus_tag="ACHUQ7_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane channel protein of phosphonate
                     transporter"
                     /protein_id="HFR1137086.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLDVLVVSWQGAEMAPLTLIKDG
                     GNMATFAVMTPTY"
     gene            complement(277..1293)
                     /gene="phnD"
                     /locus_tag="ACHUQ7_003911"
     CDS             complement(277..1293)
                     /gene="phnD"
                     /locus_tag="ACHUQ7_003911"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR1137087.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            complement(1318..2106)
                     /gene="phnC"
                     /locus_tag="ACHUQ7_003912"
     CDS             complement(1318..2106)
                     /gene="phnC"
                     /locus_tag="ACHUQ7_003912"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFR1137088.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            complement(2239..2682)
                     /gene="yjdN"
                     /locus_tag="ACHUQ7_003913"
     CDS             complement(2239..2682)
                     /gene="yjdN"
                     /locus_tag="ACHUQ7_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HFR1137089.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            complement(2840..3175)
                     /locus_tag="ACHUQ7_003914"
     CDS             complement(2840..3175)
                     /locus_tag="ACHUQ7_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFR1137090.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
BASE COUNT          718 a          861 c          942 g          864 t
ORIGIN      
        1 gtaagttgga gtcattaccg cgaaggttgc catgttgccg ccgtctttaa tcagcgtaag
       61 cggggccatt tccgcgccct gccacgagac gaccagcacg tcgagaacaa cggcccagct
      121 cagaagcgag aaccagctac gcttgggtgg ggcgatggtg atggtttgca tgtttggctc
      181 cggtctgtag gccggataag gtgcttgcac cgcatccggc atcaacgcct acacattgtc
      241 ggatgcgacg ccggggcgtc ttatccgacc tacgatttac tgcaccgctt tactcaccga
      301 actcatcgcg cttaacgcgt tgttcaggcg gtccaggtca tccagctgcg cctgaatctc
      361 ggtggttttc gccagcttgt cctgctcatt cagcccttta ttgcctttca cgctttgtat
      421 ctctttaaac agtgcgagct ggcgaatcgg caccagttgc aggtcgctgg aagcgcggaa
      481 cggcgcccag cccaggcgtt ccagcaccgc tttctcttcc ggcgtttttc cgtagttcat
      541 aaagaagtcg tagatcttgt ctttggtagt ttcggacaga ttcttgcgcc agacgatcgg
      601 atcgcctagg atcagcggcg atttccagat caccttcagc tctttaagct tctccggcgc
      661 ggaggttttc agcttgtcga ggttttcggt gttgttggtg gcaacatcca cttgcttgtt
      721 ggcgacggcc agcgcgttgg tttcatgccc ggcgttgacg gtgcgcttga agtcgctggc
      781 ggagatattg tttttagcga agacgtagta accggggacg aggaagccag aggtggagtt
      841 aggatcgcca ttgccgaagg tgagatcttt ccgcttcgcc agcagatcgt tcaggttgtt
      901 gatcgggctg tctttgttga cgatcaacac gctccagtaa cccggcgatc catccgccgc
      961 gaccgtctgg gcgaagacct ggccattggc gcgatccacc gcttccatcg ccgacaggtt
     1021 gccgtaccag gcaatatcca ctttattgaa gcgcatcccc tggataatgc ccgcgtagtc
     1081 cggggcaaag aaggcgttga ccttcacgcc cagtttcttc tccatatcct gtaaaaacgg
     1141 cgtccattgc ggtttcaggt tttgctgtga ttccgttgaa ataatgccga aattcagcgc
     1201 cttttcctgc tcttcggcgt gtgccggggt taacagggtg ctgaggctga acatgctggt
     1261 gaaggccagc gaggcaatta tcttagcgtt cattcgtttt cctcaatgat ggggatatca
     1321 ggcagctttc gcgttctctt cgatgcggtt aatgctgcgg tagagatggt caaaacgttc
     1381 gttatcaaac agttggctgc tgccgtcgta gaagacgtgc ccctgacgca gggcgacgat
     1441 gcgttcgcag tagcgcaggg cgtaatccac ctgatgcagc gtgacgacca cggtgatgcc
     1501 gtcgttctgg ttgatgtcgc gcagggtgtc catcacgatg cgcgctgatt ccgggtccag
     1561 cgaggcgatg ggttcatcgg ccagaatcac tttcgcctgc tgcatcagcg cacgggcaat
     1621 cgccacacgt tgctgctggc cgccggagag ggtggaaacg cgctgatggg caaaatgcac
     1681 catgccaacg cgggtcagcg cctgtaacgc gcgctgtttc tgctcgccgg tgaaccagct
     1741 aaaacaggtg cgccagaacg gcgtgctgcc gagcgcgcca atcagcacgt tctccagtac
     1801 gctcaggcgg ttcaccaggt tgaattgttg aaagatgtag ccggtgttgg cgcggctttt
     1861 gcggatatcg cgggccaggc ggccttcgcg ctggacagtg cggcccagta gctcgatatg
     1921 gctaccggcg gatttatcgc cggtaatcaa accgcttaaa tgacgtaaaa gggtggattt
     1981 gccggaaccc gacggcccaa gcagagccac catttcactg tgatgaatgt tcagatcaac
     2041 cgcatgcagc gcttgatgct gattgaaggt tttggcgagc ttctcgacac ggataatcgt
     2101 ttgcatgatg cagcctccct aaaaaagtgg ctccatcgtg gcggattatt gtgacgattt
     2161 ggttaattaa aagtgactaa cagatgaaga gttaacggga attcgatgac agtgcgggag
     2221 ggcgggcctc ccggcgggtt attgcgttgg ttgttgtttg acgacattaa tcatccacgg
     2281 tacgccaaat ttatcggtga ctttgccaaa gccatgcgcc cagaaagttt cctgccaggc
     2341 catttcgatt tttccgttag cggcaaggtt atcaaaccag cgttttcctt cttcgacctg
     2401 ttgcgaatcg agcaccagcg taaagccgga gtagctggct tttcctgacg gtatagcatc
     2461 gctcatcatg atgtcgcttc cggcaatgcg cacgttggca tgagcgatgg cggtatcggg
     2521 aaattgcatt ccggaagggc agttctcggc gctgtcctgc gctgatttcg gcatttcgcc
     2581 gaagctgatt ttatagagca gttccgcgcc caacgtacgt tgataatagg caatcgcgtc
     2641 ggaacagtta ccggcaaaag agaggtaggg acttaacggc atgatggtga cctcagttaa
     2701 gagaagccag ttaagtgtag ttcgaaattt atacagatga gaggctgcct gataagacgc
     2761 gcaagcgtcg catcaggcag tcggcatcgt tgccggatgc ggcgcgagcg ccttatccgg
     2821 cttaccgatc acaatacaat cagttctttt tcacaaactc agatttcagt ttcatcggac
     2881 caaaaccgtc gattttgcaa tcgatgttat ggtcgccttc aaccaggcgg atgtttttca
     2941 ctttggtgcc aattttcagc atcgaagagc tacctttcac cttcagatct ttaatgatgg
     3001 taacgctgtc gccgtcagcc agcagattgc cgttagcatc tttaacgatc agctcgtcgc
     3061 tttcctgtgc aggttctgcg tcgttccatt cgtaggcaca ttccgggcag atgtacatgc
     3121 cgttatcttc gtaagtgtat tcggagttgc attttgggca gtgtggtaat gacatgtgga
     3181 tttcctcaaa agtcagcagg gcaaaagcgc caaaaaacgg cagattgccg aaaaaggccg
     3241 caattataca caaaatccct gagtttgtcg ggggtactgc tgattttaga cgacgatcgt
     3301 tttcatttga aaggaaacgc aaaaagaaaa taatcttttt gcctctattt attatttaag
     3361 ataatgttaa tgctctactt gttct
//
LOCUS       DAZPCS010000253         3348 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZPCS010000253 DAZPCS010000000
VERSION     DAZPCS010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.259"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            19..897
                     /locus_tag="ACHUQ7_003915"
     CDS             19..897
                     /locus_tag="ACHUQ7_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR1137091.1"
                     /translation="MMTFNLREQQKRLQARMDELRAEIAFAQKGEKPWPYRSCLMREG
                     RGYCEKHGEYHTHILVWSDRNGEDREEISCCPDCLIAEANDLTMELSSIKAEELTDNA
                     GIALRFRDCEFDNYLEVNPGAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVAMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADEVIERYASMDLLIIDEV
                     GVQFGSAAEMAILQEIINARYESILPTILISNLSPEELWAFISPRIADRITDGGRNWL
                     SFNWPSYRSRIRGVAA"
     gene            894..2285
                     /locus_tag="ACHUQ7_003916"
     CDS             894..2285
                     /locus_tag="ACHUQ7_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFR1137092.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFSVRA
                     YQDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWTRFSQGVGRMTG
                     RNIWMVDQANLAIDEICATTKHHLIKYPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQARGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESARVLSTPMKPGNPRRYS
                     NKRTDSSKMERFF"
     gene            2282..2539
                     /locus_tag="ACHUQ7_003917"
     CDS             2282..2539
                     /locus_tag="ACHUQ7_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1137093.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEALR"
     gene            2634..2834
                     /locus_tag="ACHUQ7_003918"
     CDS             2634..2834
                     /locus_tag="ACHUQ7_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HFR1137094.1"
                     /translation="MSVKIQTIPELLIQTRGNMTEVSRMLNCNRATVRKYAEDKEGKG
                     HAIVDGVLIVHRGWDRGKDSDA"
     gene            2827..3207
                     /locus_tag="ACHUQ7_003919"
     CDS             2827..3207
                     /locus_tag="ACHUQ7_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFR1137095.1"
                     /translation="MRDIQQVLERWGAWVANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMVICGCMARLNRNNNDLHDLLVDYYVFGMTFMTLARKHGCSDGYIGKKLQKA
                     EGVVEGMLMMLGVRLEMDKYIEHP"
BASE COUNT          885 a          726 c          969 g          768 t
ORIGIN      
        1 cattctgagg tgacagcgat gatgacgttt aacctgcgtg aacaacaaaa aagactacag
       61 gcgcgaatgg atgagttacg ggcagagatt gcatttgctc agaagggcga aaagccatgg
      121 ccttatcgtt cctgcctgat gcgtgaaggt cgcggatatt gcgaaaaaca tggcgaatat
      181 cacacgcata tactggtgtg gagcgatcgt aatggcgagg acagagaaga aatttcatgc
      241 tgccctgact gcttaatcgc tgaggccaac gatttgacca tggagctgtc gtccatcaag
      301 gcggaagagc tgactgataa cgccggaatt gccctgcgtt ttcgggactg cgagtttgat
      361 aattatctgg aggttaatcc tggcgcagcc agaaatcttg cggcctgtcg ccgctatgcg
      421 gagaactggc cagatatgct ggagaacggt accagtcttg ttatgaccgg cagttgtggt
      481 accggaaaaa atcatctggc tgtggctatg gcaaaacaca tcatccgtaa ctatctggcc
      541 agtgtggaga tcaccgacgt gatgcgcctt acccgtgctg tgaaaaattg ctggcggaat
      601 gacagcgaaa aaacagcgga tgaagttatt gagcgttatg cgtcaatgga tttgctgatc
      661 atcgacgaag ttggcgttca gtttggcagc gcggctgaaa tggctatttt gcaggaaatt
      721 atcaacgcca ggtacgaaag tatcttgccc accattctga tcagtaacct ctcaccggaa
      781 gagttgtggg cgttcatcag tccccgaatt gccgacagga tcacagacgg gggacgcaac
      841 tggttgtcgt ttaactggcc cagttaccgt tctcgtatca gaggtgtggc tgcatgacaa
      901 ctccagtatg gcgtaacgat gaccttgaag gcgctgtcat cggcgcgttc tttctgcgcg
      961 gagcagatcc tgaagtgatg gatattctgg ccacactgcc agcggacgtt ttttctgtac
     1021 gagcgtatca ggatatctat acaggcatct gcagacaggc ccgtgtttca ggagtgattg
     1081 accccgtgct gttgtgtaat gagatgccgg aacttgcccc ggtgattact gataccgggc
     1141 gtaaaacctg ggtgaagtct tcactggagc actatgttgc agcgttgcgg cgcaatgccg
     1201 cactgcgcga tgcagaaaaa acactgaatg aggcgctgca gaaattacgt gatgcgcata
     1261 cctgtgaagc agctgaagat gccctgaagg atgcgcagaa catgatggtc acactgtcga
     1321 caggaaaggg cgtcattcag ccggtacata ttgatgatgt gcttccggaa gtggttgagc
     1381 gtgttgaatg ccggaatcag gggctggaga aatccaggac gttgatgacc ggtattgatg
     1441 aactggacgc aaaaacaggc ggtatggagc ccggagacct ggtattcatt gccgcccgtc
     1501 cttcgatggg gaaaaccgaa cttgcgctgg acatcatcga caaggtgact gagcaggggc
     1561 atggtgtgct tctgttcacc atggagatgg cgaacatcca gattggtgaa cgtatggtgt
     1621 ctgcggctgg agggatgcca gtatcacgcc tgaaatctgt ggctcacttt gaagatgaag
     1681 actggacgcg tttctcacaa ggggtggggc ggatgaccgg gcgcaatatc tggatggtgg
     1741 accaggcgaa cctggccatt gatgagatat gcgcaacaac gaaacaccac ctgattaaat
     1801 atccggaaac ggcgctggtg gtggttgatt atctcgggct gataaaaacc cgaaccacgg
     1861 ggcgtcatga ccttgccgtg ggtgaaatct caaaggggct taaaggcctg gcaaaatccg
     1921 gtggttttcc gttgattgcg ctgagccagc tctcccgcgg tgtggagtcc agacccaata
     1981 aacgtcccat gaactcagac ctgaaaaatt ccggagaaat agaggcggat gcagacatca
     2041 ttctgatgct ttacagggat gaagtgtaca acccggatac gcaggccagg ggcatcgcag
     2101 aaatcaatat cacgaaacaa cgtaacggtt ctctgggaac gatttaccgg cgtttttata
     2161 acggacattt tctgcctgtg gaccaggaaa gtgcacgggt gctttccact cccatgaagc
     2221 ccggcaatcc gcgcagatac agtaataaac gaactgacag cagtaagatg gagcgtttct
     2281 tttgaacaac cagacaatga cttttacccc tgaacaattg cgtaaacacg cgcaggaaat
     2341 gctgcgtcat gctgaacaac tcgaaaaaac gggcataaca aaagatgcta tccgtaaaga
     2401 tatggtaccc gcgcttcggg aactgatgca ggcgaagcat cgcgcacaga aagcagtaga
     2461 tgagctggtg gactgtgtgg cagagctgga aaccagagtc ggaaagtttg aaaaactggt
     2521 gcaggaggcg ctgcgctgat gcgccatgag tttgttttgc cctggcttgt gcgcgatcat
     2581 tatgtccctg gtggaaagcc cgggataaaa atcaccgaac cggagagtat ttgatgagcg
     2641 tcaaaattca aacgataccg gaactgctta tccagacacg aggtaatatg accgaagttt
     2701 cacgaatgct gaactgtaat cgcgccacgg taagaaaata tgctgaagat aaagagggca
     2761 aagggcacgc cattgttgat ggtgttctga ttgttcatcg tggatgggac aggggtaaag
     2821 acagtgatgc gtgatattca gcaggttctt gagcgctggg gcgcgtgggt ggctaataat
     2881 catgaggatg tgacctggtc gtccattgct gccggtttta agggattaat cccttcaaaa
     2941 gtaaaatccc gcccgcaatg ctgtgacgat gacgcgatgg ttatatgtgg gtgtatggcc
     3001 cgccttaacc ggaacaacaa cgatctacat gacttgctgg ttgactacta cgtgtttggg
     3061 atgacattca tgacgctggc gcggaagcat ggatgctcag atggttatat aggaaagaag
     3121 ctacaaaaag ccgagggggt ggtggaagga atgttgatga tgttgggtgt ccggttagaa
     3181 atggacaaat atattgaaca tccctgatgg taaggggcga ctttctagaa aatattttac
     3241 gtacgtaaaa aacgggatat tctgttaaga gtggttgctt cgccacacag cttaaacccg
     3301 cctccgggcg ggttttttat gcctgaaaaa cggtacagga cgttaaac
//
LOCUS       DAZPCS010000254         3325 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZPCS010000254 DAZPCS010000000
VERSION     DAZPCS010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.260"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..208
                     /locus_tag="ACHUQ7_003920"
     CDS             <1..208
                     /locus_tag="ACHUQ7_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1137096.1"
                     /translation="RHPDKRFIFNGNEIWKRGESTTEYTRAHQRTIRRLRRLIARKQY
                     TEKWFRKYDTWSAGYYELMATKPF"
     gene            269..457
                     /locus_tag="ACHUQ7_003921"
     CDS             269..457
                     /locus_tag="ACHUQ7_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1137097.1"
                     /translation="MNIYFRIVISLAIIACIYGLIVPALISMKDTVAVISGFALACLT
                     PPCIYAIYKGLSFSKDKR"
     gene            454..1281
                     /locus_tag="ACHUQ7_003922"
     CDS             454..1281
                     /locus_tag="ACHUQ7_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFR1137098.1"
                     /translation="MKKVIPALALVLLTTGLVGCDRVEPGNVGIKVNKLGDDKGVGEV
                     VGVGRYWTGWNTEVYIFPTFKQMKTYDEPFSFQMSDGTTIGYHIGVAYKVDPSKVTTV
                     FQTYRKGVDDITDTDLRQKIADALNRLASKMTTDKFIDGGKSELLDAALKDIQEEMTP
                     IGIQVMSLSYVGKPEYPPTVIDSINAKVTANQKTLQCEQEVKQREAEANMLRAEAAGQ
                     ADAIRTKAQAEADAIRLRGEALRQNPNVMELEAINKWNGTLPQYMTSGANTPFIQVK"
     gene            1325..2074
                     /locus_tag="ACHUQ7_003923"
     CDS             1325..2074
                     /locus_tag="ACHUQ7_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFR1137099.1"
                     /translation="MNTVTINNKQFPVIEYRGQRVVTLAMIDEVHQRPEGTARAAFNR
                     NREHFISGVDYAELGADVIRTDLPEGTFSKFAPSGIVLFESGYLMLTKPFNDALAWQV
                     QRELVNSYFRTRTPLTEIEMIAAIAADAVRQQKRLNHVEEQLETVTEAVETIKRGNMR
                     AGYVGYRQVVAKSGMSDTKCRNLVNAYQIPTDTHEFMTPDGLLSRRAIVELEPFMAAF
                     HQMMSEADPRGTRWYHPKMGLFQVIGWEDKA"
     gene            2071..2640
                     /locus_tag="ACHUQ7_003924"
     CDS             2071..2640
                     /locus_tag="ACHUQ7_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1137100.1"
                     /translation="MIIQSKLIRAALVCAAKNDVRYYLNGLHITPKHIEATNGSVALR
                     MAHGIRTKKNIIVQFEGGVPAKAETTELIFSKEPIAVHRDQFQRRLSITGIKLVDGCF
                     PDLDRIIPKKFDRCTHPVLQAGYLSYPEKMFGRERKFIPVQLRPSGDGQAVRIQFDSI
                     INSMYGNPEFVVMPCRDHGDFNVAQEHPE"
     gene            2637..2759
                     /locus_tag="ACHUQ7_003925"
     CDS             2637..2759
                     /locus_tag="ACHUQ7_003925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1137101.1"
                     /translation="MKIEYQDAAGGESMSWPDAIVTLGMVFAVAFVVYSICRWG"
     gene            2764..3006
                     /locus_tag="ACHUQ7_003926"
     CDS             2764..3006
                     /locus_tag="ACHUQ7_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFR1137102.1"
                     /translation="MFALIQRGQIYTDRAGYPVEITRSTEHSVFFRRMDGRTGRVRIG
                     EFSSLFEHIDHLEYHKILAETEQEKHLKKLRAMQRK"
     gene            3010..3156
                     /locus_tag="ACHUQ7_003927"
     CDS             3010..3156
                     /locus_tag="ACHUQ7_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1137103.1"
                     /translation="MNKAFELWVHQRYGNHYDLTRDVDGFYCREIVKRMFEVWCHCRG
                     LSVV"
     gene            3165..>3325
                     /gene="xisR"
                     /locus_tag="ACHUQ7_003928"
     CDS             3165..>3325
                     /gene="xisR"
                     /locus_tag="ACHUQ7_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFR1137104.1"
                     /translation="MGNVIQLAPNEWVCESVLIAITGLKPGTILRTRKECWMVGREYI
                     HVSPDGNPKP"
BASE COUNT          928 a          797 c          854 g          746 t
ORIGIN      
        1 acgccatcct gacaagcgtt ttatcttcaa cgggaatgag atatggaaac gtggagaatc
       61 aaccacagag tatacccgcg cacaccagcg aacaatcaga cgactgcgca gactcatcgc
      121 caggaaacag tatacagaaa aatggttcag aaaatatgac acatggagcg ccggatatta
      181 cgaactgatg gcaacaaaac cattctgacg taaacgaaat taaccatgac gcaattaaat
      241 aaggcaagcc gaatacatca ggaggaccat gaacatttat ttcagaatag ttatatcact
      301 ggcaattatc gcatgtattt acggattaat cgttccggcc ctcatatcaa tgaaggatac
      361 ggtagcagtg atttctggct ttgctctggc gtgtctgacc ccgccctgca tttatgccat
      421 ttataagggt ctttcttttt ctaaggataa aagatgaaaa aagttattcc tgctttagcc
      481 cttgttctgc tgaccaccgg acttgtcggt tgcgatcgcg ttgagcctgg taatgttggc
      541 atcaaggtaa acaaactggg cgacgataaa ggcgtcggag aagtggtcgg tgttgggcgc
      601 tactggacgg gatggaacac tgaggtttac atcttcccga ccttcaaaca aatgaagacc
      661 tacgatgagc cattcagctt ccagatgagt gacggtacaa ccatcggcta tcacatcggc
      721 gtggcctaca aagttgatcc atccaaagtt accacagtct ttcagaccta ccgcaaaggc
      781 gtggacgaca ttaccgacac cgacctgcgc cagaagatag ccgacgcact caaccgactg
      841 gccagcaaaa tgaccaccga caaatttatc gacggcggca agtctgagct gctggatgca
      901 gctcttaaag acattcagga agaaatgacg cccatcggta ttcaggtaat gagcctctca
      961 tatgtgggta agccggagta cccgcctact gttatcgaca gcattaatgc caaagtcacg
     1021 gcgaaccaga aaaccctgca atgcgagcag gaagtaaaac agcgcgaagc ggaagccaac
     1081 atgttgcgcg cggaagctgc cggacaggca gatgcgattc gcacaaaagc ccaggccgaa
     1141 gccgatgcta ttcgtttacg cggtgaagct ctgcgccaga acccaaacgt tatggagctg
     1201 gaagccatca acaagtggaa cggtacactg ccgcaataca tgaccagcgg tgccaacaca
     1261 ccatttattc aggttaagta atacatatgc ccggtattac acgccgggct gtctggagat
     1321 aaaaatgaat actgtaacca tcaacaacaa acagtttccg gtaatcgaat atcgcggtca
     1381 gcgtgttgtg acgctggcga tgattgatga agtccaccag cgaccggaag gaaccgcgcg
     1441 tgcagcattc aaccgaaacc gtgagcattt catcagcggt gtggattatg ccgaattagg
     1501 tgcggacgta atacgtacgg acctcccgga aggaacattc tctaaatttg caccatcggg
     1561 gattgtactt tttgaatcag gttacctgat gttgacgaag ccattcaatg acgcccttgc
     1621 ctggcaggtt cagcgcgaac tggttaacag ctacttccgc actcgcacac cgctaacgga
     1681 aatcgagatg atcgctgcga tagccgccga cgctgttcgt caacagaagc gcctgaatca
     1741 tgtggaagaa cagctcgaaa cagtcacaga agcggtggaa accatcaaac gcggaaatat
     1801 gcgcgccgga tatgtcggtt accgccaggt ggtagccaaa agtggaatga gtgacaccaa
     1861 gtgtcggaat ctggtgaacg catatcaaat tcctacagac acccacgaat tcatgactcc
     1921 ggacggtctg ttgtctcgca gggctatcgt cgagcttgag ccatttatgg cggcgttcca
     1981 ccaaatgatg tcagaggctg acccacgcgg cacacgctgg tatcacccga agatggggct
     2041 tttccaggtg atcggatggg aggataaagc atgatcatcc agtcaaaact tattcgcgcc
     2101 gctctggtgt gcgctgctaa aaacgacgtt cgttattacc tgaacggtct tcacatcacg
     2161 ccgaaacata ttgaggcaac caatggttcc gtggcactgc gcatggctca cggcatccgg
     2221 acgaagaaaa acatcattgt ccagttcgaa gggggcgtcc cggccaaagc cgaaacgaca
     2281 gagctgattt ttagtaaaga gccgatcgct gttcatcgcg accagtttca gcgacgactg
     2341 tccattaccg gcattaaact ggtggacggt tgttttccgg atttggatcg catcattccg
     2401 aaaaagtttg accgctgtac tcacccggtg ctccaggcgg gttacctgag ttatccagag
     2461 aagatgtttg gtcgtgagcg taaatttatt cccgtccagt tacgtccctc cggtgacggg
     2521 caagcggtca gaattcagtt tgattccatc atcaactcaa tgtatggcaa tcctgaattt
     2581 gttgtgatgc cttgtcgtga tcatggcgat ttcaacgtgg ctcaggagca tccggaatga
     2641 aaatcgaata ccaggacgcc gcaggaggtg aatcaatgag ctggcctgat gcaatcgtaa
     2701 ctctggggat ggtattcgca gtagcgtttg ttgtgtactc gatttgtcga tggggataac
     2761 cacatgttcg ctttgattca acgcgggcag atatacacag acagagccgg atacccagta
     2821 gaaattactc gcagtactga gcactcagtg ttctttcgga ggatggacgg acgtaccggg
     2881 cgagtacgca tcggtgaatt cagtagcctg ttcgagcata ttgaccacct ggaatatcac
     2941 aagattctgg ctgaaacaga acaagaaaag cacctgaaga aattacgcgc aatgcaaagg
     3001 aagtaaaaaa tgaataaagc gtttgagcta tgggtccacc agcgttacgg caaccactac
     3061 gacctgacgc gagatgttga cggtttctac tgtcgtgaaa ttgtgaaacg aatgtttgag
     3121 gtctggtgcc actgccgtgg gctgagtgtt gtgtgaggta atgcatgggc aatgtgattc
     3181 aactggctcc caatgaatgg gtttgtgaaa gcgttctaat cgcaattacc gggctcaaac
     3241 caggcacaat tcttcggacc cggaaagaat gctggatggt tggaagagag tatattcacg
     3301 tatcaccaga cggtaatcca aagcc
//
LOCUS       DAZPCS010000255         3315 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZPCS010000255 DAZPCS010000000
VERSION     DAZPCS010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.261"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            15..1055
                     /gene="gatD"
                     /locus_tag="ACHUQ7_003929"
     CDS             15..1055
                     /gene="gatD"
                     /locus_tag="ACHUQ7_003929"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFR1137105.1"
                     /translation="MKSVVNDTDGIVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMQGKVLLIP"
     gene            1161..1934
                     /locus_tag="ACHUQ7_003930"
     CDS             1161..1934
                     /locus_tag="ACHUQ7_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFR1137106.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(2016..2915)
                     /gene="yegS"
                     /locus_tag="ACHUQ7_003931"
     CDS             complement(2016..2915)
                     /gene="yegS"
                     /locus_tag="ACHUQ7_003931"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFR1137107.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNSLIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
BASE COUNT          888 a          727 c          825 g          875 t
ORIGIN      
        1 aatcaggagt ttttatgaaa tcagtagtga atgatactga tggtatcgtg cgcgttgcaa
       61 aaagcgtcat tcctgaaatt aaacatcagg atgaggtgcg ggtaaaaatt gccagctcgg
      121 gcttatgtgg ttccgattta cccaggatat ttaaaaatgg tgcacattat tatccaataa
      181 cgttaggcca tgaatttagc ggctatattg atgcggtggg atccggtgtt gatgatttac
      241 accctggcga tgcggttgcc tgtgtgccgt tattaccctg ttttacttgt ccagagtgtt
      301 tgaaagggtt ttattcccag tgcgcaaaat atgattttat tggctcgcgg cgtgatggtg
      361 gatttgctga atatattgtc gttaagcgaa aaaatgtctt tgctctaccc acggatatgc
      421 ctattgagga tggggctttt attgagccga ttaccgttgg cctgcatgct tttcatttag
      481 cgcaaggttg tgagaataaa aacgttatta ttattggtgc cggaaccatt ggcctgctgg
      541 ctattcagtg cgctgtcgcg ctgggagcaa agagtgtgac ggcgatcgac attagctcag
      601 aaaaactggc actggcaaaa tctttcggtg cgatgcaaac atttaacagt agcgaaatga
      661 gcgcgccgca aatgcagagc gttttacgcg aactgcgctt taatcagctt atcctcgaga
      721 cggctggcgt accgcaaact gtcgaactgg cggtagagat tgccggtcct catgcccaac
      781 tggcgctggt gggcacgttg catcaggatc tgcatttaac atcggcaacg tttggcaaaa
      841 tattgcgtaa agagctgacg gttatcggca gttggatgaa ctattccagc ccttggccgg
      901 ggcaggagtg ggaaacggcg agccggttgc tgacagaacg taagttaagc ctggagccat
      961 taatcgctca ccgtggaagc tttgaaagct tcacccaggc ggtgcgtgac atcgctcgta
     1021 atgccatgca gggcaaagtg ttgctcattc catgaaaccg cgggccagcg tgatgctggc
     1081 ccggtattgt gcaaaacaga tcattcacca atggtccccc ttcgtttaca ctagccacaa
     1141 ttgaatatgg gtaaatgacg atgaattcat tcgagcgaag gaataagatc atccaattag
     1201 tgaatgaaca gggaaccgtg cttgttcagg atctggcggg agtatttgct gcctcggaag
     1261 cgacaatccg tgccgatttg cgctttctcg aacaaaaagg cgtggttacg cgctttcatg
     1321 gcggtgcggc gaaaataatg tccggtaata gtgaaaccga gacccaggaa gtcgggttta
     1381 aagagcgatt tcagctcgcc agcgcgccaa aaaacagaat agcgcaggcg gcagtcaaaa
     1441 tgatccatga agggatgacc gttattctcg acagcggaag taccacaatg cttatcgccg
     1501 aaggattaat gaccgctaaa aatatcacgg tgattaccaa cagtctcccg gcagcgtttg
     1561 ccctttccga aaataaagac attactctgg tcgtctgtgg tggcacggtc cgccataaaa
     1621 cgcgctcgat gcatggttcc attgccgagc gttcattgca ggatattaat gccgatttaa
     1681 tgtttgtcgg tgctgatggt attgatgcgg ttaatggtat tacgaccttt aatgaaggtt
     1741 attcgattag cggggcgatg gtcaccgccg caaataaagt gattgccgtt ctcgattcat
     1801 cgaaatttaa tcgtcgcggt tttaatcagg tactgccaat cgaaaaaatt gacattattg
     1861 ttaccgatga tgctgtgtca gaggtggata aattggcatt gcagaagaca cgggtaaaat
     1921 taattacggt gtagtattgt tctgtatgcc ggataaggtg tttacaccgc atccggcatt
     1981 acggattctc cctggttcaa gagagaatag tttgattaac gcaataatgg acaatctggt
     2041 ggtaatcgac aacgcaacgc cgccggaagt atttcaatat ggaagttttg cccactcaac
     2101 ggttcgccat caagattaaa agtgatttcg tgtggggctt gtatatcaaa ccacgacgaa
     2161 gcgccttcga taatattcgg gttatcttcg tcagatttta aggttgatac gagagccgga
     2221 agaatttcat cgccggtaaa aatgcgcagt tgcagcaagc catcgttaat taacgagttc
     2281 gggcacaatt gttgaccgcc accggcctga cgcccgttac caataccaat gaccagggcg
     2341 tcaccttgcc agtgaaagtt ttcaccgcgg atttcacaac ggtccggttg cagagtatcc
     2401 atgcgcatta agccatgaat gatgtaagag acgccaccca gcgcggcttt taatttttcc
     2461 ggcgtttctg tggtaatacg cgtcccaaat ccgcctgtcg ccatattaat aaaacaggtt
     2521 tgtttgttga cctgcgccat atctatcgca atggcattac cggcaattgc cagtttcagc
     2581 gccttatcca gtgcctcagg aatccctaca ctggtggcaa aatcattggc ggttcctaat
     2641 ggcaaaattc ccagcgcggg tatgtcatcc ccctcacact gaatcaacgc cgtagaaact
     2701 tcattaatgg tgccatcgcc accaccggca atcaccgttg cgacgcccaa cttccgggcc
     2761 tcctctacaa atcgtgcggc atcacctttc tcccaggtga cccgcacatg gatcgtcatt
     2821 ccttcctcac gcaacagcat aattgcttcg cgcaagggta gattgtcagt acttttacca
     2881 ttaagaatca gtaagctggc gggaaattct gccatgatcg tatgtgcctt tatgattggt
     2941 ctgtagatag tgtagagcag aaaacaaaag gtggagtcag aacaggatga aagtcggagg
     3001 ataaaaagtc agttcaagta tggaggattc agtggttacc accaggagct tgcaatgggg
     3061 acagcgaata ctaacggctg ccaccgataa atttcaaaaa agagcatata cctaatattc
     3121 aactaaacag tggcatcttc aatataatat attaaagccc ccatggagtt accctgaagg
     3181 gcctcaatgt ccgtaattcc tacttatgta ggaaatgttg tacagaacat ttattataat
     3241 cctattcaat tataataatc atgccattat tatatttaaa cactagagag tgtcgttggt
     3301 atttaatggg ggaag
//
LOCUS       DAZPCS010000256         3254 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZPCS010000256 DAZPCS010000000
VERSION     DAZPCS010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.262"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..299)
                     /locus_tag="ACHUQ7_003932"
     CDS             complement(<1..299)
                     /locus_tag="ACHUQ7_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR1137108.1"
                     /translation="MERRCFFIINDAIIFDPNKCSLSNVNTQDEINFPATAGCILAVL
                     LESNGGAVDRNSIIDSVLERFSFDLSNNTLNQYISLLRKSFKNIGVEENVILTIP"
     gene            545..1306
                     /locus_tag="ACHUQ7_003933"
                     /pseudo
     CDS             545..1306
                     /locus_tag="ACHUQ7_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709260.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(1413..1925)
                     /locus_tag="ACHUQ7_003934"
     CDS             complement(1413..1925)
                     /locus_tag="ACHUQ7_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1137109.1"
                     /translation="MKKTLLAIMLAGTAFASQAGTLVSQGTEASANLTLTKPIVVNNT
                     IQPVKGVYSGTLTAWTPLATGIVGASDGQSHDYAVTFPDDIYAESSTSADAVISGDNN
                     PDHKLKVSLTTLEQDPPSAASEEIGGKRYMMLKNTGTGGAYRVVSHMKEQVVEPDSYT
                     IRTQAYIYAE"
     gene            complement(2064..3212)
                     /locus_tag="ACHUQ7_003935"
     CDS             complement(2064..3212)
                     /locus_tag="ACHUQ7_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR1137110.1"
                     /translation="MSITTNRYASGYATLTEAVSAQDERNRKRDKNSHDGSTISLSQP
                     LGNIGNLNFNTTRYNSSRGTGNTRSTSLSYSTVWRGITFSINWAKNDLLTSHKWKVDR
                     KLSVGISVPLSLGDENQIYASSQMSRSGEQGNNYQVSLSGQNSGGVWWDVATNITNAH
                     QSQPKSTMNIVQVGKNGSYGQFSSHYSSSENMKQLGANLSGGILITRDGLTFGQNVDG
                     TLALIEAPGATGVNVNGWPGLSTDFRGYAILPVQPYRRDDVILDEKTIGKNYDLPQTS
                     QLVVPTAGAVVPATLAVKSGDKGLVTLKQKEGKPIPFGAVISYSKDTENMAGIVGEDG
                     IAYVSGLSAEGEFNVKWGYSKDQSCIAKYQLPAKKSASGLYQIAATCL"
BASE COUNT          980 a          730 c          582 g          962 t
ORIGIN      
        1 ttgggtatcg taagaatcac attttcttca acaccaatat ttttaaaact tttgcgtaac
       61 agggatatat attggttcaa tgtgttgttt gacagatcaa atgaaaatct ctccaaaacg
      121 ctatcaatga ttgagttcct gtcaaccgct ccaccattac tttccagtaa cactgccaaa
      181 atacaccccg ccgtagctgg aaaatttatc tcatcctggg tatttacatt cgagagcgag
      241 catttgttcg gatcaaaaat aatcgcgtca ttgataatga aaaaacatct ccgctccatg
      301 caggcaacaa cacaaaataa gattttgtca ttttagccta ttcctggtgg aactgacaca
      361 agaaactaaa atacaaatat aaccctgaaa aagtcatata aaacttatat ccacagaata
      421 aaaaatcaaa catgtcagtt ttatcaatat aaagtatcgc aaggcgaaaa gtgatgcaag
      481 aatactccta agtaaatgaa agcgcaccac atgaaattat caacacatgt aatttcatca
      541 ttaggtgcta tcgaaaataa ctcaggagct acttaagatg aataatgtct tactcatgag
      601 acccgatgac tcagaattta tgatacctga tgactggtca gggctaaacg caaaaggcat
      661 tacatacgaa caagataata gagtgacaga gtcttttctg ccttttaaat ctttttattg
      721 ttcaccggtg tgcacgacag ttgaactcat ctcataaaat aattgttttg ctatgggtat
      781 ccgctacctt ctggaaaaga aaagagactt aaggctaaat cactttaaag ccttctctca
      841 taaatgcgag acaaaacctg acgtgttaat tatgaccggg tgcaccgtcg ctgatactta
      901 tagtatattc tcacgcattg ttgaagaaaa tataatgaaa acgatgacac ctatcgtcat
      961 cattactggg catcactcag aagatctttg ttctctggca tcgaaactgg gaggagttaa
     1021 aataatatca ccgacagaaa agattgcaaa aatagaaaac gctatcaatg atgcttttaa
     1081 cagcaagcct aaaaaaataa gatttttaac agatatactc tctcctcaac aaattgagac
     1141 catgaaaatg gttgcgcagg gtagatccgt taatgagatt tcagccgcaa tgaatattag
     1201 catcaagacc gtgtatgcac ataaagttcg tgctatcaat aagctgggaa ttaagaaaaa
     1261 agtttatgaa gcgctttttt ataaagcgct aattcagttc aactgatgta aaaatcaccc
     1321 tcgcttaata aataatatat tctatgtctt tttttataag ttaaaaaagg cgggatatca
     1381 aatcccgcca tgaaattaac taatcatctg cattattctg cgtagatata agcctgggta
     1441 cggatggtgt aagagtctgg ctcaacaacc tgttctttca tatgggaaac gacgcgatag
     1501 gcaccacctg ttccagtatt tttcagcatc atataacgct taccgccaat ctcttcggag
     1561 gcagctgaag gaggatcctg ctcaagcgtt gtcagggaaa ctttcagttt atggtccggg
     1621 ttattgtcac cagaaattac tgcatccgct gaagtactgc tctccgcata aatatcatcc
     1681 gggaaagtta cagcatagtc gtggctctgc ccatcagatg cgccaacaat acctgttgcc
     1741 aaaggcgtcc atgcagttaa cgtaccgcta tatacccctt tcactggttg aatggtatta
     1801 ttaacaacga taggcttggt caaagtaaga ttagctgacg cttcagtacc ctggcttact
     1861 aaagttcccg cctgagaagc aaaagctgtc cccgccagca tgattgctaa caatgttttc
     1921 ttcattatat tttctccatg attttcattt acactgcaaa atgcagtttc tttctttgca
     1981 tttacgtgct atcgcgaaaa tgcttccacc aaataatgga aacatcttca caaaaataaa
     2041 ttatgagttt cttaatttag aaactataaa catgtcgccg ctatctggta taaccctgat
     2101 gcacttttct tcgcaggaag ttggtatttt gcgatacagg attgatcttt agaataaccc
     2161 catttcacat taaactcgcc ttccgcactc aagccagaaa cataagcaat accatcttca
     2221 cctacaatgc cggccatatt ttcagtgtct ttgctataac taatcacagc gccgaagggg
     2281 atgggtttac cctctttctg ttttaaggtt actaatcctt tatccccgct cttcactgcc
     2341 aatgtcgcag gcacaacggc tccagctgtc ggtacaacca attgactagt ttgtggtaag
     2401 tcgtaattct tacctatcgt tttttcatcc agaataacat cgtctcttcg gtatggctgc
     2461 actggcagta ttgcataccc acgaaaatca gtactcagtc ccggccagcc attaacgtta
     2521 acccccgttg ctccgggtgc ctctataagg gctaatgtcc catccacatt ttgaccaaat
     2581 gtaagtccat cccttgtaat aagtatccca ccgcttaaat ttgcacctag ctgtttcatg
     2641 ttttcgctgg agctgtaatg gctactaaac tgaccatatg atccattttt gccaacttgc
     2701 acaatgttca tcgtgctctt cggttgtgac tgatgagcgt tagttatatt tgtcgctaca
     2761 tcccaccata caccaccact attctgacct gataatgaaa cctgatagtt attaccctgc
     2821 tctcccgaac gactcatctg tgaagacgca taaatttgat tttcgtcacc cagcgataat
     2881 ggcactgaaa tacccacgct taatttccga tctactttcc atttatgcga tgtcagcaag
     2941 tcgttttttg cccagttaat ggagaatgtt atgcctctcc ataccgtact ataactcagg
     3001 ctggtagacc gcgtgtttcc tgtgccccgg gaagaattat acctcgtggt attgaagttc
     3061 aaattcccga tattaccaag gggctgggat aaggatatcg tagagccatc gtgactattc
     3121 ttatctcttt ttctgttccg ttcatcctgt gcacttactg cttcagtcag tgtggcataa
     3181 cctgatgcat aacggttcgt ggttatagac aatagactgg ccccctgaat ctccagacaa
     3241 ccagtatcac ttat
//
LOCUS       DAZPCS010000257         3190 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZPCS010000257 DAZPCS010000000
VERSION     DAZPCS010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.263"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            170..1273
                     /gene="proB"
                     /locus_tag="ACHUQ7_003936"
     CDS             170..1273
                     /gene="proB"
                     /locus_tag="ACHUQ7_003936"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFR1137111.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRRNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1285..2538
                     /gene="proA"
                     /locus_tag="ACHUQ7_003937"
     CDS             1285..2538
                     /gene="proA"
                     /locus_tag="ACHUQ7_003937"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFR1137112.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            2653..2728
                     /locus_tag="ACHUQ7_003938"
     tRNA            2653..2728
                     /locus_tag="ACHUQ7_003938"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2686..2688,aa:Thr,seq:cgt)
     gene            complement(2743..>3190)
                     /locus_tag="ACHUQ7_003939"
     CDS             complement(2743..>3190)
                     /locus_tag="ACHUQ7_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1137113.1"
                     /translation="ESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKESCTKPDGT
                     KAPTVRKMRYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVLQEMGGWE
                     SIEMVRRYAHLAPNHLTEHARQIDLILNPSVPNLSQSRNKEGTNDV"
BASE COUNT          752 a          807 c          878 g          753 t
ORIGIN      
        1 tgtggtaatt tattaaatct gtaataaaag cgtaaacaac tgccgctacg cttgctgatc
       61 ccgcgcaaca aaacgccatg ctttgctcgc agatggttgg caaccgacga cagtcctgct
      121 aaaacgttcg tttgatatca tttttcctaa aattgaatgg cagagaatca tgagtgacag
      181 ccagacgctg gtggtaaaac tcggcaccag tgtgctaaca ggcggatcgc gccgtctgaa
      241 ccgtgcccat atcgttgaac ttgttcgcca gtgcgcgcag ttacatgccg ccgggcatcg
      301 gattgttatt gtgacgtcgg gcgcgatcgc cgccggacgt gagcacctgg gttacccgga
      361 actgccagcg actatcgcct cgaaacaact gctggcggcg gtagggcaga gtcgactgat
      421 tcaactgtgg gaacagctgt tttcgattta tggcattcac gtcgggcaaa tgctgctgac
      481 ccgtgctgat atggaagacc gtgaacgctt cctgaacgcc cgcgacaccc tgcgtgcgtt
      541 gctcgataac aatatcgttc cggtaatcaa tgagaacgat gctgtcgcta cggcagagat
      601 taaggtcggc gataacgata acctttctgc gctggcggcg attctggcgg gtgccgataa
      661 actgttgctg ctgaccgatc aaaaaggttt gtataccgct gatccgcgca gaaatccgca
      721 ggcagaactg attaaagatg tttacggcat tgatgacgca ctgcgcgcga ttgccggtga
      781 cagcgtttca ggcctcggaa ctggcggcat gagtactaaa ttgcaggccg ctgacgtggc
      841 ttgccgtgcg ggtatcgaca ccattattgc cgcgggcagc aagccgggcg ttattggtga
      901 tgtgatggaa ggcatttccg tcggtacgct gttccatgcc caggcgactc cgcttgaaaa
      961 ccgtaaacgc tggattttcg gtgcgccgcc ggcgggtgaa atcacggtag atgaaggggc
     1021 aactgccgcc attctggaac gcggcagctc cctgttgccg aaaggcatta aaagcgtgac
     1081 tggcaatttc tcgcgtggtg aagtcatccg catttgcaac ctcgaaggtc gcgatatcgc
     1141 ccacggtgtc agtcgttaca acagcgatgc attacgccgt attgccggac accactcgca
     1201 agaaattgat gcaatactgg gatatgaata cggcccggtt gccgttcacc gtgatgacat
     1261 gattacccgt taaggagctg gccgatgctg gaacaaatgg gcattgccgc gaagcaagcc
     1321 tcgtataaat tagcgcaact ctccagccgc gaaaaaaatc gcgtgctgga aaaaatcgcc
     1381 gatgaactgg aagcacaaag cgaaatcatc ctcaacgcta acgcccagga tgttgctgac
     1441 gcgcgagcca atggccttag cgaagcgatg cttgaccgtc tggcactgac gcccgcacgg
     1501 ctgaaaggca ttgccgacga tgtacgtcag gtgtgcaacc tcgccgatcc ggtggggcag
     1561 gtaatcgatg gcggcgtact ggacagcggc ctgcgtcttg agcgtcgtcg cgtaccgctg
     1621 ggggttattg gcgtgattta tgaagcgcgc ccgaacgtga cggttgatgt cgcttcgctg
     1681 tgcctgaaaa ccggtaatgc ggtgatcctg cgtggtggta aagaaacctg tcgcactaac
     1741 gcggcaacgg tggcggtgat tcaggacgcc ctgaaatcct gtggcttacc ggcgggtgcc
     1801 gtgcaggcga ttgataatcc tgaccgtgcg ctggtcagtg aaatgctgcg tatggataaa
     1861 tacatcgaca tgctgatccc gcgtggtggc gctggtttgc ataaactgtg ccgtgaacag
     1921 tcgacaatcc cggtgatcac aggtggtata ggcgtatgcc atatttacgt tgatgaaagt
     1981 gcagagatcg ctgaagcatt aaaagtgatc gtcaacgcga aaactcagcg tccgagcaca
     2041 tgtaatacgg ttgaaacgtt gctggtgaat aaaaacatcg ccgatagctt cctgcccgca
     2101 ttaagcaaac aaatggcgga aagcggcgtg acattacacg cagatgcagc tgcgctgaca
     2161 cagttgcagg caggccctgc gaaggtggtg gcggttaaag ccgaagagta tgacgatgag
     2221 tttctgtcat tagatttgaa cgtcaaaatc gtcagcgatc ttgacgatgc catcgcccat
     2281 attcgtgaac acggcacaca acactccgat gcgatcctga cccgcgatat gcgcaacgcc
     2341 cagcgttttg ttaacgaagt ggattcgtcc gctgtttacg ttaacgcctc tacgcgtttt
     2401 accgacggcg gccagtttgg tctgggtgcg gaagtggcgg taagcacaca aaaactccac
     2461 gcgcgtggcc caatggggct ggaagcactg accacttaca agtggatcgg cattggtgat
     2521 tacaccattc gtgcgtaaat aaaaccgggt gatgcaaaag tagccatttg attcacaagg
     2581 ccattgacgc atcgcccggt tagttttaac cttgtccacc gtgattcacg ttcgtgaaca
     2641 tgtcctttca gggccgatat agctcagttg gtagagcagc gcattcgtaa tgcgaaggtc
     2701 gtaggttcga ctcctattat cggcaccatt taaatcaata agttacacat cattagtacc
     2761 ttccttattt cttgactggg acaaatttgg gaccgatggg ttcagaatca agtctatttg
     2821 ccgtgcgtgt tcggtaaggt gattaggtgc gaggtgagca tatcgacgaa ccatttcgat
     2881 agactcccag cctcccattt cctgtaacac tgataacggg actccggctt gaaccagcca
     2941 acttgcccag gtgtgtctca agtcgtgaaa tctgaaatca tcaataccag cccgtctcag
     3001 cgccgctttc caggctgtgt ttgcgtcata ccgcatcttc cttactgttg gcgctttcgt
     3061 tccgtctggt ttggtacagc tttccttgta cacaaatacc caacggtgat gatttccgat
     3121 ttgttttttc aatacgcgac atgcagtatc attcagcgca acgccaattg cgcggtttga
     3181 tttactctct
//
LOCUS       DAZPCS010000258         3179 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZPCS010000258 DAZPCS010000000
VERSION     DAZPCS010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.264"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            418..1527
                     /gene="wzz(fepE)"
                     /locus_tag="ACHUQ7_003940"
     CDS             418..1527
                     /gene="wzz(fepE)"
                     /locus_tag="ACHUQ7_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFR1137114.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVDFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
     gene            complement(1772..1906)
                     /locus_tag="ACHUQ7_003941"
     CDS             complement(1772..1906)
                     /locus_tag="ACHUQ7_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR1137115.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAATGKQDVREMY
                     "
     gene            complement(2552..2722)
                     /locus_tag="ACHUQ7_003942"
     CDS             complement(2552..2722)
                     /locus_tag="ACHUQ7_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFR1137116.1"
                     /translation="MNMARCIKSQSLTLLEKLDALDADEQAAMCKRLHELAEELQNSI
                     QIRFEAESETGT"
BASE COUNT          801 a          659 c          774 g          945 t
ORIGIN      
        1 aaaaaaaaca ttatcttata ttaggggtgc tgctagcggc gcggtgtgtt tttttacagg
       61 acgcccctgg gggcgctgct aggggtgtct gttcagatat gtagcaatga gtcagggatt
      121 cacgaactga aaatgtccga aaatgtgtct catgggcact gtatgcgccc aaaccgatcg
      181 tcattcactt tcatgcatag ctatgcagtg agctgaaagc gatcctgacg catttttccg
      241 gtttaccccg gggaaaacat ctctttttgc ggtgtttgcg gcagaatcgc gttcagcgcg
      301 ttttagcggt gcgcgcaata aaatgttatg ttaaatgtct gctgaatggt ttttaggttt
      361 tttcctaaca tatgatagaa taaaaaaacc atatgttaat atattgtgtt tttttcaatg
      421 aaaatctcaa aggtctctga cgaacatttt tctaattaca acatgttgcc gaatggtaat
      481 gaagaaattg acctttttgg cttgattagc gttttatggg cagcaaaaat aaaaattgtt
      541 ggggtgattc tattttttgc ttgtattggc tttcttgttt cttttgtttt gccacaaaaa
      601 tggactagtt ctgctgtggt tactcctgct gagtctgttc aatggagtga attaaataag
      661 gagctttcca agcttcatgt tctgggagtg gattttgata tcaatcgtga tagcgcattt
      721 gctctgttta ttaagaaatt tcagtccgtt aattcgttga atgagtatat gagatcatct
      781 ccttatgtta tggagattat taaaaagaat aacattagcg ctctcgaact gcatcgtgca
      841 attgttgagt tgagtaaaaa tatgaagtcg attgatgata atgccagtaa gaaaaacgac
      901 aagtcatcat tgtatgtatc atggactctg agttttactg ctccaacaag taaagaagct
      961 cacgatgtgt tgtctgggta tattaattat gtttcttccc ttgttgtaag ggatttgatg
     1021 gaagatataa gaaataaact agaagttaaa actaatgttg aaaaagaaat tcttgcactg
     1081 gatgagataa aaattagaaa ccagctgaat gcagatattc gacgcctcaa ttattcactg
     1141 gaggttgcta atgcggctgg aataaaaaaa cctgtataca gcaatggtca gattatgaag
     1201 gatgacccag attttcctgt ggctctcggt tctgatggta tagcaactaa attgaacatc
     1261 aaaaaatcaa tcaaggatgt ttcggaattg agtggggagt tgcgaaatcg tcaatatgtt
     1321 gtgaatcaat tggttgtggc gaaagtgggg gatgttgatt ttatgccatt tcagtatcaa
     1381 ttgagcccaa cgttaccggt cagaaaggat gggccaagta atgccataat tgtaattctt
     1441 tcttctgtca ttggagggat gcttgcatgt ggctatatac ttttacgtca tgccatggag
     1501 gccagaaaga tgagtgtgat tgagtaaaag ttgtgctgat gcatcatttt ataagcaact
     1561 tttataaaaa gttcaaagga tcttcttgag atcattccct tacagccgta gtcttctact
     1621 ctagaaaacg aaaaaaccac ctggggaggt ggtttttcga aggttcagta agttggggaa
     1681 cttctgaacc gtggtaacag tcttgtgcga gacatgtcac caaatctgtc cttctagtgt
     1741 agcctcagta aggctgccac ttcaagaact ctcaatacat ctctcgcaca tcctgtttac
     1801 cagtggccgc tgccaatggc gttaagtcgt gtcttaccgg gttggactca agacgatagt
     1861 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     1921 agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat gagacgaacg cggccataac
     1981 agcggaatga caccggtaaa ccgaaaggca ggaacaggag agcgcacgag ggagccacca
     2041 gggggaaacg cctggtatct ttaagtcctg tcgggtttcg ccacctctga cttgagcgtc
     2101 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccacc ggcgcggcct
     2161 tttttcgggg cctctttttt gctcacatgt tttttctggt gttatcccct gattctgtgg
     2221 ataaccgtat atccgtcttt gcgtgagcag acaccgctcg ccgcagccga acgaccgagc
     2281 gcagcgagtc agtgagcgag gaagcggaag agcgccggaa gctgcatttt ctccttacgt
     2341 atctgtgcag catttcacac ctcatatgtc gctgttctct caccatctgc tctgccgcct
     2401 cataattaag ccagtataca ctccgttatc gctacgtgac tgggtcatgg ctgcgccccg
     2461 acacccgcta aaaccccctg acgcgccctg acgggcttgt ctgctgacga aaacgcccac
     2521 gggaaagccg tgaccgcctc cgtcagcttc gttatgttcc tgtttcactt tcggcctcaa
     2581 agcgaatctg gatgctgttc tggagttctt ctgcgagttc gtgcagtcgt ttgcacatgg
     2641 ccgcctgttc gtcggcatcc agagcatcca gtttttcaag cagtgtcagg ctctggcttt
     2701 tgatacatcg cgccatattc agcgcagttt gttgttattt gttcaccttc cgttgttctc
     2761 cgtcagttcc gggtgtcggg ggggggaccc tgagcaggtg gtaattgtaa gacgttgcgg
     2821 gcgacggtat tacaattgca catcctgtcc tgtttcttta ggacgtatca tggcacagca
     2881 tatcaactga taaataatcc ctgtgcagtg cagccgctgt tgcataaaat ttcgctctgc
     2941 gtcgagtgtt tcttaatcaa taattccgct gatactccgg aaaaacgcga gtctgttctc
     3001 atgcctgaaa tgcccacacc ccacgcaaaa acaagttttt gctgattttt ctttataaat
     3061 agagagttat gacaaattag ttcttcttgc tctctttgtg atatttaaaa aagcggtgtc
     3121 ggcgcggtgt tgtagctgcg ccaacaccgc tttttagggg tggtactgac tattttcat
//
LOCUS       DAZPCS010000259         3104 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZPCS010000259 DAZPCS010000000
VERSION     DAZPCS010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.265"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            95..2248
                     /locus_tag="ACHUQ7_003943"
     CDS             95..2248
                     /locus_tag="ACHUQ7_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HFR1137117.1"
                     /translation="MYKSLFTIIILLYSASSYAKSESPPASTITFIAQIDHVELNKTA
                     LSHDMKLVLADRFYFKTSNPCETNSISARPESMDITDKAYADKLKKLETPFLDDRYFF
                     MTINQCDGNGTPMLTDIKVCTTELCGAEYTKKMSTVWLDETLKPTAKRLAIYFIQSPL
                     PYDEEKKQWKVTGWYISSTDNQSSTKQIAFEGYTDTDNLSDSKFISSFKSWFESGKIK
                     NIANYNPKGERDGKYESYYNSNGNVYESLSFSNGLINGEFITYYENGAIESKRHFNND
                     KIKDGECPHFYEDGKIKQQHSYLNSKLDGPAYEYYPDGKLMQENFYQQSVLIGNDTSY
                     YQSGKISSIHNRNSRGQYDGANERYSEEGKLLSKSVYKDGKQISVQTWYENGQKEEEK
                     HFDEQGQLNGLVKQWYKNGNLAKSQNYKHDILDGNSEEWYENGIPESLYPYKNGKTDG
                     IAKSWNKYGKLTYSIEYKNGVENGAYRNWSKNTGKLTKETLYVNGIRQGVEKEFNDRT
                     GKLLTSTQYVNNKRHGTEETYDQNGIKYITCYQNDQKLSSLDNPTQIKDNATTGDSSA
                     QFALGKYEFICANIDEGIKWLTKSAEQKNTDAIYFLATAYKGNGIPANNEKYLAYLQQ
                     AATLGSSNAQAEIGYLYLIGKELPQNLPDAGVWFKKAAAQGNFAAHFYLGRMYQNGDG
                     VERNMEKARFHLSNAAEGGIEPALKALNELEHQTK"
     gene            2301..>3104
                     /locus_tag="ACHUQ7_003944"
     CDS             2301..>3104
                     /locus_tag="ACHUQ7_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1137118.1"
                     /translation="MKAKSTLLLMILSVFYSHQVLSSEPTQPDVYSVVEKKLNSALPL
                     EENTTFRSQAEWFNLQYELLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEEA
                     PETALTFLSKIGLNKPATLTSFESYINSWIKSKQPEKAMQLLSLDENALNYYLPTVLV
                     AYQIVPDKAISIYNSVYGDNVVIPYQQVTMLLTVAEGYQTKGDIKNAIHYADKALNMF
                     DKAIAVDSSRESHYSDEYLKLMNIYAANGNKEKAVVLSQRLHKAITESQS"
BASE COUNT         1128 a          562 c          572 g          842 t
ORIGIN      
        1 tcgcttaatt ccatataact ggacgattac attagaagta aactatactt tttatgaaat
       61 atcagggata aattattcac atggaaaata catcatgtac aaatcattat ttactattat
      121 cattctactg tatagcgcca gttcatatgc aaaaagcgaa tcccctcccg caagcactat
      181 aactttcatt gcacaaatcg atcatgttga gctaaataaa acagcattat ctcatgatat
      241 gaaactcgtt cttgccgatc gtttttattt caaaaccagt aatccttgcg aaacaaattc
      301 aatttctgct cgaccagaat caatggatat tactgacaaa gcctatgccg acaaattaaa
      361 aaaattagaa actccttttc ttgatgatcg ctatttcttt atgaccatca atcagtgtga
      421 cggtaatggc actcccatgc tgacagacat aaaagtatgt acaacagaat tatgtggtgc
      481 tgaatataca aagaaaatgt ctactgtatg gctcgatgaa actttaaaac caactgctaa
      541 acgtctggca atttatttta ttcaatctcc tttaccctat gacgaggaga aaaaacaatg
      601 gaaagtcacc ggatggtata tctcatcaac tgataatcag tcttcaacta aacagattgc
      661 atttgaagga tatacagaca cagataacct ctccgattct aaatttattt cttcatttaa
      721 atcctggttt gaaagtggaa agataaaaaa cattgctaac tataatccaa aaggagaaag
      781 agatggaaaa tacgaaagtt attacaatag caatggtaac gtctatgaat ctctaagttt
      841 ttctaatggt cttatcaacg gtgagtttat tacatattat gaaaatgggg ccattgaaag
      901 taaacgtcat tttaataacg acaaaattaa agatggtgaa tgccctcatt tttatgaaga
      961 tgggaaaatc aaacagcaac atagctatct gaatagtaaa ctcgatggtc ctgcatatga
     1021 atattatcct gatggcaaac tcatgcagga aaatttctat cagcaaagtg tgttgattgg
     1081 aaacgatacc agttactacc aatccggaaa aataagctct atacacaata gaaattcccg
     1141 tggtcagtat gatggagcta acgaacgtta tagtgaagag ggtaagttac tgtctaaatc
     1201 cgtctataaa gacggaaaac aaatttctgt tcaaacctgg tatgaaaatg ggcagaaaga
     1261 agaggagaaa cattttgatg agcaaggcca attaaatggt ctggttaagc aatggtataa
     1321 aaatggtaat cttgcaaaat cacaaaacta taaacatgac atactcgatg gtaacagtga
     1381 agagtggtac gaaaatggca tccctgaaag tttgtaccct tataagaatg gaaaaaccga
     1441 cggcattgcc aaatcatgga ataagtatgg taaattaacc tattcaatcg aatacaaaaa
     1501 tggcgtagag aatggggcat atcgtaactg gagcaaaaac acaggtaaac taaccaaaga
     1561 aaccctgtac gtcaatggta tcagacaggg agtggaaaaa gagtttaacg atcgcacagg
     1621 taaactatta acctccacgc aatatgttaa taataaaaga catggtacgg aggaaactta
     1681 tgatcaaaac ggaataaaat atattacatg ctatcaaaat gatcagaaat tatcgtcttt
     1741 agacaatcca acacaaataa aagataatgc aacaacaggc gattccagtg ctcaatttgc
     1801 attagggaaa tacgaattca tctgtgcaaa tatagatgaa gggattaaat ggttaaccaa
     1861 atctgcagag caaaaaaaca ctgatgcgat atacttttta gctacagcct acaaaggcaa
     1921 tggtatacct gcaaacaatg aaaaatacct agcatatctg caacaagctg caacactggg
     1981 cagtagtaat gcccaggcgg aaattggcta tttatatctc atcggaaaag aactaccaca
     2041 gaatcttcct gatgctggtg tctggttcaa aaaagcagct gcacaaggta attttgccgc
     2101 acatttctat ctgggaagga tgtatcagaa tggtgacggc gttgagagaa atatggaaaa
     2161 agccaggttt catctatcta atgcggcaga aggtggaata gaacctgcgc ttaaagcatt
     2221 aaatgaactt gaacatcaaa caaaataaag cgctcaggga ccacgtaagg ttcatcaaac
     2281 agaaaaggat atagtttgcg atgaaagcga aatccacttt gttattaatg attttatctg
     2341 ttttttattc tcatcaggtt ttatccagcg aacccactca gccggatgtt tattctgtcg
     2401 tcgagaaaaa actaaactct gctttacctc tggaagaaaa taccaccttc agatcgcagg
     2461 ctgaatggtt taatttgcaa tatgaattat tagtatctgg ctaccctgaa cgtgcttaca
     2521 atttactgag caaacttgag acagaatcga aaaacgctaa atattatctg gatttaactg
     2581 cctccagagt cgctgaagaa gaagcaccag agacagcctt aactttttta tcaaaaattg
     2641 ggttaaataa accggctact cttacatcct ttgagagcta tatcaatagc tggataaaaa
     2701 gcaaacaacc cgaaaaggct atgcagcttc tcagcctcga tgagaatgcc ctgaattatt
     2761 atttaccgac tgtgttagtc gcatatcaga tcgttcctga taaggcaata agtatttata
     2821 attctgtata tggagataat gttgttatac cctatcagca agtcaccatg ttacttacag
     2881 ttgcggaggg ttatcaaaca aaaggcgata taaaaaacgc cattcattat gccgataagg
     2941 cgctgaatat gtttgataaa gctattgctg tagatagcag tcgtgaatcc cattatagtg
     3001 atgagtattt gaaactcatg aatatctacg ccgccaatgg caacaaagaa aaagcggtgg
     3061 ttttatctca acgtctacac aaagccatta ccgaatctca atcg
//
LOCUS       DAZPCS010000260         3069 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZPCS010000260 DAZPCS010000000
VERSION     DAZPCS010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.266"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..498
                     /locus_tag="ACHUQ7_003945"
     CDS             <1..498
                     /locus_tag="ACHUQ7_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1137119.1"
                     /translation="DGEILKSACEINDSDKKIEVALGHYNAEQFRSVGDRSPKIPFTI
                     PLVNCPVTGWEHDNGNVEASFRLWLETRDNGTVPNFPNLAKVGSFAGTATTGVGIRID
                     DAESGNLMPLNAMGNDNTIYQIPADSNGIVNVDLIAYYVSTVEASEITPGEADAVVNV
                     TLDYR"
     gene            578..1273
                     /locus_tag="ACHUQ7_003946"
     CDS             578..1273
                     /locus_tag="ACHUQ7_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR1137120.1"
                     /translation="MSKRTFAVILTLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGKSRAPFIITPPLFRLEASDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRVKMFYRPAHLKGRVNDAWKSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1302..>3069
                     /locus_tag="ACHUQ7_003947"
     CDS             1302..>3069
                     /locus_tag="ACHUQ7_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR1137121.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYAFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWGYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNSNISVGVSGQQWGIGYSLNYQYSRYTDENNDRALSL
                     NLSIPLERWWICPYISRHLLSLN"
BASE COUNT          888 a          718 c          684 g          779 t
ORIGIN      
        1 gatggcgaga ttttaaaatc cgcttgtgaa atcaatgact ctgataagaa aattgaagtt
       61 gctcttggtc actataatgc cgaacagttt cgttctgttg gcgatcgtag cccaaaaatt
      121 ccattcacta ttcctttagt taactgccca gtgactgggt gggagcacga taacggcaac
      181 gttgaagcgt ctttccgttt atggctggaa acgcgggata atggcaccgt ccctaacttc
      241 cctaatctgg caaaagtggg ctcttttgct ggcacagcga caacaggtgt gggtatccgt
      301 attgacgatg cggaaagcgg aaaccttatg ccactgaatg ctatggggaa tgataacacg
      361 atttatcaga tcccagcgga ctccaatggt attgtcaatg ttgacctcat cgcttactac
      421 gtatcaaccg ttgaagcatc agaaatcacc ccaggggaag cagacgctgt cgttaacgta
      481 acgctggatt accgttaata cgttacggcg ttatctgacc tttcagataa cgcccttttc
      541 cttcctcttt ctcgttgtat caggttgaaa aataactatg tcaaaacgaa cattcgcggt
      601 gatattaacc ttgttgtgta gcttctgtat cggccaggcg cttgcagggg gtatcgtttt
      661 acagcgaacg cgagtgatct atgatgccag ccgcaaagag gctgcgttac ctgtcgcaaa
      721 caaaggcgca gaaacgcctt atttactgca atcatgggta gataatatag atggtaaaag
      781 ccgtgcccca tttattataa ccccaccgct atttcgtctt gaggctagcg atgactcatc
      841 actgcgaatt attaaaacag ctgataacct gcctgaaaat aaagagtcgc tgttctacat
      901 taatgttcgt gccattccag caaagaaaaa atcagatgat gttaatgcta acgagttgac
      961 gctggtattt aaaacgcggg tcaaaatgtt ttatcgcccc gcacacctga agggacgggt
     1021 aaacgatgcg tggaaatcac tggaatttaa acgtagtgac cattcactca atatatataa
     1081 cccaactgaa tattacgtcg tatttgccgg actggcagtc gataaaaccg atctcacaag
     1141 caaaattgaa tatatcgcgc ccggagaaca taaacagtta ccacttccag catctggcgg
     1201 aaagaacgtg aaatgggctg cgatcaacga ttatggcggc agttccggga cagaaactcg
     1261 tccactgcaa taaaaaatat aaaaacacag gccatcaggg aatgccacaa cgacaccacc
     1321 agggacataa acgcacaccg aaacagttgg cgctcattat caaacgttgt ttgccgatgg
     1381 tgctcactgg cagcggcatg ctttgcacta ccgctaacgc cgaagagtat tatttcgacc
     1441 ccattatgct ggaaactaca aaaagtggta tgcaaacaac cgatctgtca cgtttttcaa
     1501 aaaaatacgc acaactacca ggaacttatc aggttgatat ctggctgaat aaaaagaagg
     1561 tttcacagaa aaaaattaca tttaccgcca atgcagagca acttctgcag ccacagttta
     1621 cggtagaaca actacgtgag ctgggtatta aggtggatga aatcccggcg ctggctgaaa
     1681 aagatgacga tagcgtgatc aactcgcttg aacaaatcat tcccggtaca gctgctgaat
     1741 ttgatttcaa tcatcagcga cttaatttga gcattcccca aattgcactg taccgtgatg
     1801 caagaggtta cgtctcccct tctcgttggg acgatggtat accaacgctg tttaccaact
     1861 actcgtttac aggttctgat aaccgttacc gccagggcaa tcgtagccaa cgacagtacc
     1921 tgaatatgca aaatggtgct aattttggcc cctggcgatt acgtaactat tctacgtgga
     1981 cacgcaacga tcaggcgtca agctggaaca ctatcagtag ttatttacaa cgtgatatca
     2041 aggcgttgaa gtctcagttg cttctgggag aaagcgccac cagcggcagt attttttcca
     2101 gctacgcctt tactggcgtg caactcgctt ccgacgataa tatgttgcca aacagccagc
     2161 gcggatttgc cccaacggta cgcggtatcg caaacagtag tgcaatcgtg actatcaggc
     2221 aaaatggtta tgtgatctat caaagcaacg tgccagcggg tgcctttgaa attaacgatc
     2281 tctacccctc ttccaacagc ggcgatttag aagtcacgat tgaagaaagt gacggtacac
     2341 aacgtcgctt tatccagcct tattcttcat tacccatgat gcagcgacct gggcatctaa
     2401 aatatagcgc gaccgctgga cgctatcgcg ctgatgcaaa cagtgatagc aaggaacccg
     2461 aatttgctga agccacggca atatatggtt tgaataatac ttttacgctg tatggcggcc
     2521 tgctcggttc tgaagattat tatgcgctgg ggatcggtat cggcggcaca cttggcgcac
     2581 tgggcgcgtt gtcgatggat atcaacagag ctgacaccca attcgataac cagcactctt
     2641 ttcatggcta tcaatggcgt actcagtaca tcaaagatat cccggaaacc aacaccaata
     2701 tcgctgttag ctactatcgc tataccaacg atggctattt tagttttgat gaagccaata
     2761 cccgcaattg gggctataac agtcgccaaa aaagtgaaat tcaattcaac atcagccaga
     2821 caatatttga tggggtaagt ctgtatgcct ccggttcaca gcaagactat tggggcaata
     2881 acgagaaaaa cagtaatatc tctgttgggg tttccggcca gcaatgggga attggttaca
     2941 gcctgaatta tcaatacagc cgctacactg atgaaaataa tgaccgtgca ctctctttga
     3001 atctcagtat tccgttagaa cgctggtgga tttgccccta tatttccaga catctgttat
     3061 cacttaacc
//
LOCUS       DAZPCS010000261         3045 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZPCS010000261 DAZPCS010000000
VERSION     DAZPCS010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.267"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..106)
                     /gene="pliG"
                     /locus_tag="ACHUQ7_003948"
     CDS             complement(<1..106)
                     /gene="pliG"
                     /locus_tag="ACHUQ7_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFR1137122.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGH"
     gene            348..641
                     /locus_tag="ACHUQ7_003949"
     CDS             348..641
                     /locus_tag="ACHUQ7_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFR1137123.1"
                     /translation="MFCAIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            713..1372
                     /locus_tag="ACHUQ7_003950"
     CDS             713..1372
                     /locus_tag="ACHUQ7_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFR1137124.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEKRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1464..1910
                     /locus_tag="ACHUQ7_003951"
     CDS             1464..1910
                     /locus_tag="ACHUQ7_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFR1137125.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2117..3017)
                     /gene="hlyE"
                     /locus_tag="ACHUQ7_003952"
                     /pseudo
     CDS             complement(2117..3017)
                     /gene="hlyE"
                     /locus_tag="ACHUQ7_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
BASE COUNT          798 a          656 c          722 g          869 t
ORIGIN      
        1 tatggccttt gctgaactca acattgacat tcttacccgc agcaaagctc gtggaggtta
       61 gcagtgcaag cagcaataca gccttactga tgctcttgat tttcattggt agtctcctgt
      121 tcagaatgag tacgatgaac tgttataata taacaatccc ttacgggaga attccagtgg
      181 cttttctgcg ttcggtaaaa tcccggcgcg gcattttttg ccggggggat tacttataaa
      241 ggggcatgga ctcaacaaac cgcagaaatc tacagctggc tggcttgtgg cgcgggtttc
      301 attgatgtta gcttatgcca aaacctggca tcttaaagag taaaagtatg ttttgtgcga
      361 tttatcgaag cagcaagcgt gaccagacct atttatatgt cgaaaaaaaa gacgattttt
      421 cgcgtgttcc tgaggaactg atgaaaggtt ttggtcagcc tcagttagcg atgattctgc
      481 cgttggatgg gcggaagaaa ctggtcaatg ctgatattga aaaggttaaa ctggcattaa
      541 ccgagcaagg ttactatttg cagttaccgc caccacccga agatttgctg aagcaacatc
      601 tttccgtcat ggggcagaaa acagacgaca ctaacaaata accgatatcc ggcggtggca
      661 ttatctttgt cggcgcgggt tttcatatcc acgataaggt gaggggaacg ttatgtatca
      721 acatcacaac tggcaaggtg cgctgctgga ttatccggtg agtaaagtag tctgtgttgg
      781 cagtaactat gccaaacata ttaaagagat gggcagcgca gtgcccgaag agccagtgct
      841 gtttattaaa ccagaaacgg cactgtgcga tctgcggcag ccattggcga tcccatccga
      901 tttcggttca gttcatcatg aagtcgaact ggcggtgttg attggcgcga cgctgcgtca
      961 ggcaacggaa gagcatgtcc gcaaagccat tgccggttac ggcgtggcgc ttgatctgac
     1021 attgcgtgat gttcagggaa aaatgaagaa agccgggcag ccgtgggaaa aggctaaagc
     1081 gtttgataac tcttgtccgc tttccgggtt tatccccgcg gcggaattta ccggcgatcc
     1141 acaaaataca acgctgggcc tgagcgtaaa cggcgaaaaa cgccagcaag gtacgactgc
     1201 ggacatgatc cataaaatcg ttccgttgat cgcttatatg agcaagtttt ttaccctcaa
     1261 ggccggtgac gttgtgttga caggcacgcc tgatggcgtc ggcccgttgc aaagcggtga
     1321 tgagctgaca gtcactttcg atgggcattc tttgacaact cgcgttttgt aatacttttt
     1381 gccgccagaa agcggcggca aaacttgcat cgctgtgcca gactggttat aaggtgcgtt
     1441 ttaacgtaat ggcggaacac ctgatgagcg atgtaccttt ctggcaaagt aaaaccctgg
     1501 acgaaatgag cgatgcggaa tgggagtcgt tgtgtgatgg ttgcggtcag tgttgcttgc
     1561 ataaactgat ggatgaagac accgacgaaa tctacttcac taacgtcgcc tgtcgccagc
     1621 tcaatattaa aacctgtcaa tgtcggaact acgaacgtcg tttcgagttt gaacccgact
     1681 gcattaaatt aacccgtgaa aatctgccaa cattcgaatg gctgccaatg acctgcgctt
     1741 atcggttgct ggcggaaggt aaagatttac ctgcgtggca tccgctactt actggttcga
     1801 aagcggcaat gcatggtgaa cgtatctctg tgcgtcatat cgcagtgaaa gaatcagaag
     1861 tgattgactg gcaggatcat atcttaaata aacctgactg ggcgcagtga taaatttaat
     1921 agatgctgat gggggctttc ttaatatacg tctggatgct cgccatttcg tagccctgtt
     1981 tttgaaagcg ctctgtcata atgagagtta cccgatacct tattatcaac aggtaactct
     2041 caatctttaa aaaacgtctg tgtttttaaa tgtgataaac cttatacctt gagtacatgg
     2101 agagaataat cgcttatcag acttcaggta cttcaaagag tgtcttttta ccgtgtcttt
     2161 tctgatactc attacaggtg ttaatcattt ttttggccgc tgcttttagc aaagaaagca
     2221 ttaaatcatc ataatcaaca tagaatctgg ttgtttcagt ttccgttttt atctccccga
     2281 tggcggctat ttcggtggtt aatttcaatt tggcggcatc gatatcttta ttcgcttgtt
     2341 taaccgtgtt agacagggtg gtaaagaaac tctgcacaga ttttaacttg ttcttcaatt
     2401 ctggaatcag tttcccttca actacgcccg cagcaataga ataggaaatg attaaaccaa
     2461 atggaccggc gacgacatcg gctgcggcac cggcatacgc ttccttcctg attttatcta
     2521 cctgtgactg gaaatagctg cttttttctg aaaaatcatt ggttaactgg ctatctaacg
     2581 ccagcagttt cccggaagcg ttgttgaaac tttgtgagct taccagcagg gatttttgcg
     2641 cttcattcag cttcgtgatg ccgtcatcca gtaccttaat gaggggcgga tgctttcttc
     2701 tcattgtact catcaaatag caaaatatac gctgcgagca attgcgtcgc aacaccacac
     2761 cattcataca ctgtttgggt tgcttcaaaa tacttatcct ggctatccat aagtaaggtt
     2821 ttaatatcgc cgactaaaac ggaggctgcc tgtgaatact cctgtttaaa gcgacttaac
     2881 tcttttatgg tttcatcaaa ggtctgccag gggatgacct gatcgagata tttattataa
     2941 agatctaatg ctccatctgc ggtttcgatt gcgtttttaa ctacttctac cgttttatct
     3001 gcaacgattt cagtcataat cattcgcctc tttaaatata taaat
//
LOCUS       DAZPCS010000262         2998 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZPCS010000262 DAZPCS010000000
VERSION     DAZPCS010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.268"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..2850
                     /locus_tag="ACHUQ7_003953"
     rRNA            <1..2850
                     /locus_tag="ACHUQ7_003953"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          793 a          661 c          932 g          612 t
ORIGIN      
        1 gacgtgctaa tctgcgataa gcgtcggtaa ggtgatatga accgttataa ccggcgattt
       61 ccgaatgggg aaacccagtg tgattcgtca cactatcatt aactgaatcc ataggttaat
      121 gaggcgaacc gggggaactg aaacatctaa gtaccccgag gaaaagaaat caaccgagat
      181 tcccccagta gcggcgagcg aacggggagc agcccagagc ctgaatcagt gtgtgtgtta
      241 gtggaagcgt ctggaaaggc gtgcgataca gggtgacagc cccgtacaca aaaatgcaca
      301 tactgtgagc tcgatgagta gggcgggaca cgtggtatcc tgtctgaata tggggggacc
      361 atcctccaag gctaaatact cctgactgac cgatagtgaa ccagtaccgt gagggaaagg
      421 cgaaaagaac cccggcgagg ggagtgaaaa agaacctgaa accgtgtacg tacaagcagt
      481 gggagcacgc ttaggcgtgt gactgcgtac cttttgtata atgggtcagc gacttatatt
      541 ctgtagcaag gttaaccgaa taggggagcc gaagggaaac cgagtcttaa ctgggcgtta
      601 agttgcaggg tatagacccg aaacccggtg atctagccat gggcaggttg aaggttgggt
      661 aacactaact ggaggaccga accgactaat gttgaaaaat tagcggatga cttgtggctg
      721 ggggtgaaag gccaatcaaa ccgggagata gctggttctc cccgaaagct atttaggtag
      781 cgcctcgtga attcatctcc gggggtagag cactgtttcg gcaagggggt catcccgact
      841 taccaacccg atgcaaactg cgaataccgg agaatgttat cacgggagac acacggcggg
      901 tgctaacgtc cgtcgtgaag agggaaacaa cccagaccgc cagctaaggt cccaaagtca
      961 tggttaagtg ggaaacgatg tgggaaggcc cagacagcca ggatgttggc ttagaagcag
     1021 ccatcattta aagaaagcgt aatagctcac tggtcgagtc ggcctgcgcg gaagatgtaa
     1081 cggggctaaa ccatgcaccg aagctgcggc agcgacgctt atgcgttgtt gggtagggga
     1141 gcgttctgta agcctgtgaa ggtgtgctgt gaggtatgct ggaggtatca gaagtgcgaa
     1201 tgctgacata agtaacgata aagcgggtga aaagcccgct cgccggaaga ccaagggttc
     1261 ctgtccaacg ttaatcgggg cagggtgagt cgacccctaa ggcgaggccg aaaggcgtag
     1321 tcgatgggaa acaggttaat attcctgtac ttggtgttac tgcgaagggg ggacggagaa
     1381 ggctatgttg gccgggcgac ggttgtcccg gtttaagcgt gtaggctggt tttccaggca
     1441 aatccggaaa atcaaggccg aggcgtgatg acgaggcact acggtgctga agcaacaaat
     1501 gccctgcttc caggaaaagc ctctaagcat caggtaacat caaatcgtac cccaaaccga
     1561 cacaggtggt caggtagaga ataccaaggc gcttgagaga actcgggtga aggaactagg
     1621 caaaatggtg ccgtaacttc gggagaaggc acgctgatac gtaggtgaag cgacttgctc
     1681 gtggagctga aatcagtcga agataccagc tggctgcaac tgtttattaa aaacacagca
     1741 ctgtgcaaac acgaaagtgg acgtatacgg tgtgacgcct gcccggtgcc ggaaggttaa
     1801 ttgatggggt tagcgcaagc gaagctcttg atcgaagccc cggtaaacgg cggccgtaac
     1861 tataacggtc ctaaggtagc gaaattcctt gtcgggtaag ttccgacctg cacgaatggc
     1921 gtaatgatgg ccaggctgtc tccacccgag actcagtgaa attgaactcg ctgtgaagat
     1981 gcagtgtacc cgcggcaaga cggaaagacc ccgtgaacct ttactatagc ttgacactga
     2041 acattgagcc ttgatgtgta ggataggtgg gaggctttga agtgtggacg ccagtctgca
     2101 tggagccgac cttgaaatac caccctttaa tgtttgatgt tctaacgtgg acccgtgatc
     2161 cgggttgcgg acagtgtctg gtgggtagtt tgactggggc ggtctcctcc taaagagtaa
     2221 cggaggagca cgaaggttgg ctaatcctgg tcggacatca ggaggttagt gcaatggcat
     2281 aagccagctt gactgcgagc gtgacggcgc gagcaggtgc gaaagcaggt catagtgatc
     2341 cggtggttct gaatggaagg gccatcgctc aacggataaa aggtactccg gggataacag
     2401 gctgataccg cccaagagtt catatcgacg gcggtgtttg gcacctcgat gtcggctcat
     2461 cacatcctgg ggctgaagta ggtcccaagg gtatggctgt tcgccattta aagtggtacg
     2521 cgagctgggt ttagaacgtc gtgagacagt tcggtcccta tctgccgtgg gcgctggaga
     2581 actgaggggg gctgctccta gtacgagagg accggagtgg acgcatcact ggtgttcggg
     2641 ttgtcatgcc aatggcactg cccggtagct aaatgcggaa gagataagtg ctgaaagcat
     2701 ctaagcacga aacttgcccc gagatgagtt ctccctgacc ctttaagggt cctgaaggaa
     2761 cgttgaagac gacgacgttg ataggccggg tgtgtaagcg cagcgatgcg ttgagctaac
     2821 cggtactaat gaaccgtgag gcttaacctt acaacgccga agatgttttg gcggattgag
     2881 agaagatttt cagcctgata cagattaaat cagaacgcag aagcggtctg ataaaacaga
     2941 attagcctgg cggccgtagc gcggtggtcc cacctgaccc catgccgaac tcagaagt
//
LOCUS       DAZPCS010000263         2951 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZPCS010000263 DAZPCS010000000
VERSION     DAZPCS010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.269"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            141..2009
                     /gene="kup"
                     /locus_tag="ACHUQ7_003954"
     CDS             141..2009
                     /gene="kup"
                     /locus_tag="ACHUQ7_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFR1137126.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGGEVLYADMGHFGKFSIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2176..2595
                     /gene="rbsD"
                     /locus_tag="ACHUQ7_003955"
     CDS             2176..2595
                     /gene="rbsD"
                     /locus_tag="ACHUQ7_003955"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFR1137127.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2603..>2951
                     /locus_tag="ACHUQ7_003956"
     CDS             2603..>2951
                     /locus_tag="ACHUQ7_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR1137128.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWK"
BASE COUNT          658 a          701 c          779 g          813 t
ORIGIN      
        1 aagggggggc tttttctttg agccaggtta atgtggccgc atttaggagt acgattttgc
       61 cgttaatcgt gcatactgtg cgcttttttg tgggccaagg gactaagcac acatttcata
      121 tttcaacgaa agactagtct atgagcactg ataataagca atcattgccc gcgattaccc
      181 tcgcggcgat tggagttgtc tacggcgata ttggtaccag cccgttatat acacttcgtg
      241 aatgtttgtc cggccagttt ggttttggcg ttgaacgcga tgccgtgttt ggctttttat
      301 cgctgatctt ctggctgcta atctttgtgg tttccattaa atatctcacc ttcgtgatgc
      361 gggcagataa cgccggtgag ggggggatcc tgacgttgat gtcgcttgcc gggcgtaata
      421 cgtcggcgcg aaccacatca atgctggtga ttatggggct aatcggcggc agctttttct
      481 atggtgaagt cgtcataaca cccgctattt cggtgatgtc agccattgaa ggtctggaaa
      541 tcgtcgcccc gcagctggat acctggatag ttcccctctc aattatcgtt ctcactttat
      601 tatttatgat tcaaaagcat ggcacggcta tggtcggtaa gctgtttgcg ccgatcatgc
      661 tgacctggtt tttgattctg gcaggactgg ggttacgtag cattattgct aacccggaag
      721 tgctgcatgc gctgaatccg atgtgggcgg tgcatttctt ccttgaatac aaaacggttt
      781 cttttattgc attaggggca gtggtgctgt cgattacggg gggcgaggtg ctgtatgctg
      841 atatggggca ctttggtaag ttctctattc gcctggcgtg gttcaccgtc gtattacctt
      901 ccttgaccct taattacttc ggtcagggag cgttgttgtt aaagaacccg gaagcgatta
      961 agaacccgtt cttcctgttg gcaccggact gggcgctgat cccactgctg atcatcgccg
     1021 cactggcgac ggtaattgcc tcgcaggcgg ttatctctgg cgtcttctca ttgacgcgtc
     1081 aggcggtacg tctgggatat ttgtcgccga tgcgcattat tcacacctcc gaaatggagt
     1141 cagggcaaat ctatattccg tttgtgaact ggatgctcta tgtcgcggtc gtgattgtga
     1201 ttgtcagctt tgagcactcc agcaacctgg cggcggcgta cgggattgcg gtgaccggaa
     1261 ccatggtact gacgtctatt ctctcgacta ccgtggcacg tcagaactgg cactggaata
     1321 agtattttgt tgcgctgatc ctgattgctt tcctttgtgt cgatattcca ttgttcaccg
     1381 ctaacctcga taaactgctc tccggcggct ggttgccatt gagcctcggt actgtgatgt
     1441 ttatcgtgat gaccacctgg aagagcgagc gtttccgctt gctgcggcgg atgcatgaac
     1501 atggtaactc tctggaagcg atgattgctt cgctggagaa atcaccgcct gttcgcgtgc
     1561 ccgggaccgc ggtgtatatg tcgcgtgcaa tcaacgtcat tccctttgcg ctgatgcata
     1621 accttaaaca taacaaggta ttgcatgagc gggtgattct gttaactctg cgcaccgaag
     1681 acgcgccata tgtccataac gtccgtcggg tacagattga acaactgtcg cccactttct
     1741 ggcgcgtggt ggcaagttat ggttggcgag aaacgccaaa cgtggaagaa gttttccacc
     1801 gctgcggtct ggaaggatta agttgccgga tgatggaaac ctccttcttt atgtcgcatg
     1861 agtcgttgat cctcggcaaa cgcccgtggt atttgcgtct gcgcggcaag ctgtacttgc
     1921 tgctgcaacg taatgcgctg cgtgcaccag atcaatttga aatcccgcca aacagggtta
     1981 tcgaactggg tactcaggtc gaaatctaac gccagacgcc tcctttcttc ataagggggc
     2041 gtttttgttt tcatggttaa tcaccatgta aaacgtttcg aggttgatca catttccgta
     2101 acgtcacgat ggttttccca actcagtcag gattaagctg cggatcagcg aaacgtttcg
     2161 ctgatggaga aaaaaatgaa aaaaggcacc gttcttaatt ctgatatttc atcggtgatc
     2221 tcccgtctgg gacataccga tacgctggtg gtgtgtgatg ctggtttacc catccccaaa
     2281 agtacaacgc gtatcgatat ggcattaacc caaggtgtac cttcttttat gcaggtgctg
     2341 ggcgtcgtca caaatgaaat gcaggtcgag gctgccatta tcgcggaaga gatcaaacaa
     2401 cataatccgc aactccacga aacgttgctc actcaccttg agcagctgca aaaacaccag
     2461 ggaaatacca ttgaaattcg ttacaccacg catgaacaat tcaaacaaca aaccgcagaa
     2521 agtcaggcgg taattcgcag cggagaatgt tctccgtatg cgaatatcat tctctgtgct
     2581 ggcgtgacgt tctgaggccg tcatggaagc attacttcag cttaaaggca tcgataaagc
     2641 cttcccgggc gtaaaagccc tctcgggcgc agcgttaaat gtctatccgg gccgtgtgat
     2701 ggcgctggtg ggcgaaaacg gcgcgggtaa atccaccatg atgaaagtgc ttactggtat
     2761 ctatactcgc gatgccggca cgcttttatg gctggggaaa gaaacgacat ttaccgggcc
     2821 gaaatcttcc caggaagccg ggattgggat tatccatcag gaactgaacc tgatcccgca
     2881 gttgaccatt gccgaaaaca ttttcctcgg tcgtgagttt gttaatcgct ttggcaaaat
     2941 tgactggaaa a
//
LOCUS       DAZPCS010000264         2944 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZPCS010000264 DAZPCS010000000
VERSION     DAZPCS010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.270"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            284..1669
                     /gene="dcuC"
                     /locus_tag="ACHUQ7_003957"
     CDS             284..1669
                     /gene="dcuC"
                     /locus_tag="ACHUQ7_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFR1137129.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(1710..2051)
                     /locus_tag="ACHUQ7_003958"
                     /pseudo
     CDS             complement(1710..2051)
                     /locus_tag="ACHUQ7_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(2389..2898)
                     /locus_tag="ACHUQ7_003959"
     CDS             complement(2389..2898)
                     /locus_tag="ACHUQ7_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFR1137130.1"
                     /translation="MKSANEINQWMTPKQITELDGMPGTIQGVHKRAKKEGWPKRSQE
                     GRRGPGVEYIPAIPAMQKIAIEEMNADTLKMFSLLINKLGENEVREIELNMAKYGLSG
                     LMQERPSISVDTLLATLGIDRQTLQTALALHKLPPETRQEILSMYGVHKQEEPVAPLL
                     EPQDAKKAV"
BASE COUNT          680 a          666 c          690 g          908 t
ORIGIN      
        1 tgctcatatt agattatttt ttgagcgcac agccgcagct aacaccattg caattaacaa
       61 atttgcatca atccaccatc aatttacaca cattattatg tgataattgc caaccgctaa
      121 atatgcgttt tgttatctat gtataaaaac agcaacttca atgtcttaat ggcagttttt
      181 cttgatttta atcagcattc atcgccaatt tattgggcat attttttcct taagctttag
      241 gaagttttta ttatttactt tgggcctgga gacaggaaaa attatgctga cattcattga
      301 actccttatt ggggttgtgg ttattgtggg tgtggctcgc tacatcatta aagggtattc
      361 cgccactggt gtgttatttg tcggtggcct gttattgctg attatcagtg ccattatggg
      421 gcacaaagtg ttaccgtcca gccaggcttc aacaggctac agcgccacgg atatcgttga
      481 atacgttaaa atattactaa tgagccgcgg cggcgacctc ggcatgatga ttatgatgct
      541 gtgtggcttt gccgcttaca tgacccatat cggcgcgaat gatatggtgg tcaagctggc
      601 gtcaaaacca ttgcagtata ttaactcccc ttacctgctg atgattgccg cctattttgt
      661 tgcttgtctg atgtcactgg ccgtctcttc cgcaaccggt ctgggggttt tgctgatggc
      721 aaccctgttt ccggtgatgg taaacgttgg tatcagtcgt ggcgcagcag ctgccatttg
      781 tgcctccccg gcggcgatta ttctcgcacc gacttcaggg gatgtggtgc tggcggcgca
      841 ggcttccgaa atgtcgctga ttgacttcgc cttcaaaacg acgctgccta tctcaattgc
      901 tgcaattatc ggcatggcga tcgcccactt cttctggcaa cgttatctgg ataaaaaaga
      961 gcacatctct catgaaatgt tagatgtcag tgaaatcacc accactgccc ccgcgtttta
     1021 tgccattttg ccgttcacgc cgatcatcgg tgtactgatt tttgacggta aatggggtcc
     1081 gcaattacac atcatcacta ttctggtgat ttgtatgctg attgcctcca ttctggagtt
     1141 cctccgcagc tttaataccc agaaagtttt ctctggtctg gaagtggctt atcgcgggat
     1201 ggcagatgcg tttgctaacg tggtgatgct gctggttgcc gctggggtat tcgctcaggg
     1261 gcttagcacc atcggcttta ttcaaagtct gatttctatc gctacctcgt ttggttcggc
     1321 gagtatcatc ctgatgctgg tattggtgat tctgaccatg ctggcggcag tcacgaccgg
     1381 ttcaggcaat gcgccgtttt atgcgtttgt tgagatgatc ccgaaactgg cgcactcttc
     1441 cggcattaac ccggcgtatt tgactattcc gatgctgcag gcgtcaaacc tgggtcgtac
     1501 cctatcaccc gtttctggcg tagtcgttgc ggttgccggg atggcgaaaa tctcaccatt
     1561 tgaagtcgta aaacgcacct cggtgccggt gcttgttggg ctagtgattg ttatcgttgc
     1621 tacagagctg atggtgccag gaacggcagc agcggtcaca ggcaagtaaa tagtaatgcc
     1681 ggaggcaagt ttcctccggc tttatcatgt cacccactgt ggtatatgcg ttgtggtctg
     1741 ccaactttgc cgtgaacaat ttcagcaata atcagatggc ggctggcgca atattcaaga
     1801 taacgcctgg cagtggtgcg gctgatggtc agtgcctgcg ccaccgtttc tgccgtatgt
     1861 tgcacaccag gctctttaaa cagttttcgc accgcgttta gcgtcaaggg gtcaatgccg
     1921 gtcggcagtt cgtctttggg ttcaccgcga gcataggcat taaacatctc atcaatttgc
     1981 ttctggctgg cgctatcaat actttccagc atatgtttac gctggcggaa acgggttagc
     2041 gtttgcccca gtgtattgat tcacttgaag tacgaaaaaa ccgggagaac attggattat
     2101 tcgggatctg atgggattag atttggtggg gcttgcaagc ctgtagtaca aattttaggc
     2161 gttaaccaat gaaacgcgaa agatagtaaa aaattgcttt tgtttcattg aaaaaacgaa
     2221 aaacaaaaac actataaatc atttcaatca tagcttcaaa aaacgttcaa aaccgataac
     2281 aaccaggctg tcatcaaatg actcatatca caaatcagct catgccgttt aggtatgtta
     2341 catgtgtgat tatgtgaggt gaagtatgtt ttagctggct catggttgtt atacggcttt
     2401 tttcgcatct tgtggttcca acaaaggagc tacaggctct tcctgtttat gaacaccata
     2461 cattgacaaa atctcttggc gagtttctgg aggtaattta tgcagcgcca aagctgtttg
     2521 tagtgtttgg cgatcaatac ccagcgttgc caatagggtg tcaacggaga tggaggggcg
     2581 ttcttgcatt aatccggaaa gcccatattt agccatgttt agctctatct ctcggacttc
     2641 attttctcct agtttattga ttaacaggga gaacattttc aatgtatcag cattcatttc
     2701 ttcgatagca attttttgca tagccggtat tgccgggata tattcaaccc caggcccgcg
     2761 acgcccttct tgtgaacgct tcggccatcc ttctttcttt gctcgtttat gcaccccctg
     2821 tattgtccca ggcattccat ccagttcagt aatttgtttg ggtgtcatcc actggtttat
     2881 ctcatttgcg cttttcataa actaagttta ccagatttat ctatataaaa atcaatggat
     2941 taca
//
LOCUS       DAZPCS010000265         2941 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZPCS010000265 DAZPCS010000000
VERSION     DAZPCS010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.271"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..593)
                     /locus_tag="ACHUQ7_003960"
     CDS             complement(<1..593)
                     /locus_tag="ACHUQ7_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HFR1137131.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     ADLYFNLRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQ"
     gene            complement(660..1385)
                     /gene="fimC"
                     /locus_tag="ACHUQ7_003961"
     CDS             complement(660..1385)
                     /gene="fimC"
                     /locus_tag="ACHUQ7_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFR1137132.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENMLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(1422..1961)
                     /locus_tag="ACHUQ7_003962"
     CDS             complement(1422..1961)
                     /locus_tag="ACHUQ7_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1137133.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(2026..2574)
                     /gene="fimA"
                     /locus_tag="ACHUQ7_003963"
     CDS             complement(2026..2574)
                     /gene="fimA"
                     /locus_tag="ACHUQ7_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFR1137134.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTIHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
BASE COUNT          771 a          719 c          644 g          807 t
ORIGIN      
        1 ttctgtacac tatttccgct gaaattataa ttgagcaatc cggcattaat accgggatcc
       61 cataactcag gaggaatata accacgcgcg cgattactca taaatgcctg agggatcgtc
      121 aggttcagtc gctgctgacc aacatccaga tgcgcagtag cgtcctggac cattgtggtt
      181 aatggcacac aggcatcatc cgccagcaga ttcataccgg cgacagaagc cgtattcagc
      241 cccatactgg cgagttgcgc gcgtgtcagg cagggaacaa tcccttgttc actgtcgccc
      301 gtattaaatg tgacatcacg cgttgccata taaccattat tcaaatagat atcgacgcga
      361 tacgtccctg gcggtaattc ttgcccattt tcaaaacgcg ataaatcggc cacagcctgg
      421 ggatcatccg ctaaaaagcg cagattaaaa tagaggtcgg cagatgacaa aggtgcctgt
      481 gcggcaaaag cacaggcgac aacgagtcgg acaaaaaaac cagccaaacg atgcttacga
      541 atatgcaagc attgtgtgtt tcgctggtaa agtcttaaat tcagatatga cattattcct
      601 ccggtttaaa atcacccggc agtcaattct ttttattcag gcgaaaaatt ccccctgcgt
      661 tattccatta cgcccgtcat tttgggggta agtgcgccat aatcatttat tgttcggtaa
      721 gtaatattgc ttcctgcatc agaaggcaat ttaaccgtgc tttcgcccat tggaggcacc
      781 aatgcatttt caagaacccg ggttccggca ttcaactctg ttaccgtcag gtaatagggt
      841 gtcgggttaa tcagcgtcag agaattcgcg ctacgacgaa atcttaattt ttctgcggcc
      901 tgatcgggtg gcaacgctaa tttagccggg cgatagtaca gtttaatgcg gctgataatt
      961 gcgagctgta gcatattctc agtcaatttt gatttatcca ttgacggaat cgctttaacg
     1021 ttcatccaga ataaactttc ccggtcctgt ggcaattggt tatttgttgc atcaagaata
     1081 cgtaaggtat tctctttttt tcccttcatc gcaaacagag gaggcgtcac gataaaacga
     1141 ccatccttta caccatcggc attttccacc catgattgaa ttaaataggt actattttca
     1201 tcattatttg tcacggcaag ttgcacttgt ttttgccctg ccggataaat tacgcgagtc
     1261 gcacctaagg ccactcctgc ttcagcgcgt ccggcaacca tcattgccat gaacatcagg
     1321 atacctgcca gcaagcagaa tgttatttcc tgcgatttcc ttacattgac gtttttatta
     1381 ctcactgtcc tgttcctgtt atcacattat ctactgaaca attactgata ggttaaagag
     1441 aaccaggcct gggcattggc gatgccgcca gtaacccgac gcccggtagc acgatatttg
     1501 gcgatgaaat gtagcgaagt agagcctgag taaagccgtt tccagtttgc tggaggacga
     1561 ttaatcggta cgaggtttcc ttcatcatca aacaacgcta caccaatatt ggaggctatc
     1621 cctggcccct ctcccacgga gagcacatcc ggatttttac catccgcgac accgtgaaac
     1681 gccacaccca cacgttcact caccaccgtg ctacattccc gtaaatgaat aacaaaaggc
     1741 accggtgcgc tatcttcccc tgccgcatga aaccggttac tgctgatttg ccccatattg
     1801 accgtcattt gtttatcacc ggcttcaatc cggcaagttt ccgcaataat gacgccctga
     1861 aattgcatat ttccgccggg cagcgtggta ttccatttat ttccggccag agcaaacatt
     1921 ggcagcaacg acgctaacag aaataatctc ttccttttca tcgttatttt tatcgcacaa
     1981 gggtgggcat ccctgcccgt aatgacgtcc ttgaacctgg gtaggttatt gatactgaac
     2041 cttgaaggtt gcatccgcat tagctgcacc cggggttgcc tcgccgattg cataataacg
     2101 cgcctggaac ggaatggtgt tggtaccgtt attcagggtt gtttgtgcac taaatgtcgc
     2161 accgtccagc gtcagagcat tgcctgttct gtctaggatc tgcacgccaa cgtttgttgc
     2221 actacctgca gcagaactct gcagagccag tacatcagta cgcgtagcat caattgccgt
     2281 acctaagaag gcaacagcgg cttttgtggc aacagtggta tcgcaatcat tcagctgaat
     2341 gttaaaacca acggcagagc tggttgctcc agcctgcttc aggctagcgg tacgaacctg
     2401 tcccaactga acggtttgat caacagagcc tgcatcaact gcgcaagcgg cgttaacaac
     2461 ttccccttta aagtgaatgg tcccaccatt taccgtcgta gtatcggcca gagccgctgc
     2521 ggaactgagg gacagagccg acagaacaac gattgccaga gttttaattt tcatgctgct
     2581 ttcctttaaa aaaactattt ctaaatcgac atgggcagtc gttctgtaca ctttgttttg
     2641 tcaacgagtt tgactgccaa cactgcacag ttttccccca aaagatgaaa catttggggc
     2701 cattttgact catagaggaa agcatcgcgc acaaactttt tcagtttatt tgttggctta
     2761 atattctatt attatcttta tttatagatt tttatattgc atgaggtggt ttttggagag
     2821 aagaatgagg aagatgcgtc gagccacaga aacgttagct ttacatatag cggaggtgat
     2881 gtgaaattaa tttacaagag aaataattta catatcaaat agttagatgc tttttgtcgt
     2941 t
//
LOCUS       DAZPCS010000266         2935 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZPCS010000266 DAZPCS010000000
VERSION     DAZPCS010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.272"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            8..409
                     /gene="spa15"
                     /locus_tag="ACHUQ7_003964"
                     /gene_synonym="spaK"
     CDS             8..409
                     /gene="spa15"
                     /locus_tag="ACHUQ7_003964"
                     /gene_synonym="spaK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone Spa15/SpaK"
                     /protein_id="HFR1137135.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNGVL"
     gene            413..1705
                     /gene="spaL"
                     /locus_tag="ACHUQ7_003965"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
     CDS             413..1705
                     /gene="spaL"
                     /locus_tag="ACHUQ7_003965"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SpaL"
                     /protein_id="HFR1137136.1"
                     /translation="MSYTKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IVARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGIKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMAAAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELIGETIR"
     gene            1786..2136
                     /gene="spa13"
                     /locus_tag="ACHUQ7_003966"
                     /gene_synonym="spaM"
     CDS             1786..2136
                     /gene="spa13"
                     /locus_tag="ACHUQ7_003966"
                     /gene_synonym="spaM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Not similar to SpaM from Salmonella T3SS; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein Spa13"
                     /protein_id="HFR1137137.1"
                     /translation="MYRDVEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVK
                     KHIIQYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLI
                     LDELSQEDMKYGIR"
     gene            2123..>2935
                     /gene="spa32"
                     /locus_tag="ACHUQ7_003967"
     CDS             2123..>2935
                     /gene="spa32"
                     /locus_tag="ACHUQ7_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HFR1137138.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIASNYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSS"
BASE COUNT          984 a          424 c          609 g          918 t
ORIGIN      
        1 tttaaatatg agtaacatta atttagttca attagttaga gatagtcttt tcacgattgg
       61 ttgtcctcca tcaattatta cagatttaga cagccattca gcgattacca tttctttgga
      121 tagtatgcct gctattaata tagctcttgt caatgagcag gttatgttat gggctaattt
      181 tgatgcacca agtgatgtaa aacttcagtc atctgcttat aacatattaa atttaatgtt
      241 aatgaacttt agttatagca ttaatgagct ggtagagctt catcgttctg atgagtactt
      301 acagttaaga gttgttatta aagatgacta cgttcacgat gggattgttt ttgcagagat
      361 tctgcatgag ttttatcaga ggatggaaat cttaaatggg gtcttataat caatgagcta
      421 tacaaaattg ctcactcaat tatcttttcc taatagaatc tcggggccaa tcttggaaac
      481 aagtcttagc gatgtttcga ttggtgagat ttgtaacatt caggctggaa ttgaaagtaa
      541 tgaaattgtt gcaagagctc aggttgtagg atttcatgat gaaaaaacaa tattaagctt
      601 gattggaaat tctcgtggac tttcacggca aacgttgatt aagcccactg cccagtttct
      661 tcatacgcaa gtcggccgtg gattattggg agcagtagtc aatcctttag gggaggttac
      721 tgataagttt gctgttacag ataacagtga aattctttat cgacctgtag ataatgctcc
      781 tccgctatat agtgaaaggg ctgcaattga gaagcctttt ttaacaggta ttaaggttat
      841 tgattcttta ctcacgtgtg gtgaaggaca gcgaatgggg atttttgcgt cagctggttg
      901 tggcaaaact tttctcatga atatgctcat tgaacatagt ggtgctgata tatatgttat
      961 tgggttaatt ggtgagcgag gtcgagaggt tactgaaacg gttgattatt tgaaaaactc
     1021 tgagaaaaaa agcaggtgtg ttttagtata tgcaacttcg gattactctt cggttgatcg
     1081 ttgtaatgct gcatatatag ccactgctat agccgaattt tttaggactg aaggacataa
     1141 agtagcgctt tttattgatt cattaacaag gtatgccaga gcattacgtg atgtggcctt
     1201 agccgctgga gaatcacctg ccagaagagg ctatccggtt tcggtttttg atagcttacc
     1261 cagacttctt gaaaggccag gaaagttaaa ggcaggtggc tctattactg cattttacac
     1321 tgttcttttg gaggatgatg attttgctga tccattagct gaagaggtaa gatccatttt
     1381 agatggacat atatatttga gcagaaatct agcccaaaaa ggacaatttc ctgcaattga
     1441 ttccttaaaa agtataagca gggtatttac acaggttgtt gatgaaaaac atcgtattat
     1501 ggccgctgca tttcgggagc tactttctga aatagaagag ctaaggacaa ttattgactt
     1561 tggtgaatac aaaccggggg agaatgcctc tcaggataaa atctacaaca aaatatctgt
     1621 tgttgagagt tttctgaagc aagattatcg actcggtttt acttatgagc agacaatgga
     1681 gcttattggt gaaacaatta gataaggttt taaaaatcaa agataaatat caacggtctg
     1741 ttaaattaat tgaagcacac atattaacac taaaaaaaaa actctttgta tcgagatgtg
     1801 gaggcattag ataaaaggat tatatacttc ctgcaactgg agaatgactt agaacctgta
     1861 ggcgctcaga gtgttagtca gcttttcaat acaagacgta agatcgctat agtaaaaaag
     1921 catattatcc aatatcagtc tgaacgaatt cttctaaagg gaagaattga agaaattcaa
     1981 aaagatattg atgaggcaaa tgcttctaaa agaaaactgc ttcataaaga gagtaaaata
     2041 tgtaaacgga ttggcttgat taaaagaaat aactttgcca aacagttgat acttgatgag
     2101 ttatcacaag aggatatgaa gtatggcatt agataatata aacctaaatt ttagtagtga
     2161 caaacagata gaaaaatgtg agaaactatc ttcaatagat aatattgata gtctcgtttt
     2221 gaagaagaag aggaaggttg aaattcctga gtactcttta attgcatcta actattttac
     2281 tattgataag cactttgaac ataagcatga taaaggagaa atttatagtg gcattaaaaa
     2341 tgcgttcgaa cttagaaacg aacgagcgac atattctgat attccagaat caatggccat
     2401 taaagaaaat attttgatac cagatcaaga tatcaaagca agggaaaaaa taaatatcgg
     2461 cgatatgagg gggatctttt catataataa gagtggaaat gcagacaaga acttcgaaag
     2521 aagtcatact tcttctgtaa accctgataa tctgctagaa tctgataata gaaatggtca
     2581 aattggttta aaaaatcata gcttgtctat tgataagaat attgctgaca tcatttcttt
     2641 actaaatgga agtgttgcta aatcatttga gctgcctgta atgaataaaa atactgcaga
     2701 cataacccca tccatgtcat tgcaagaaaa atcaatagtt gaaaatgata aaaatgtttt
     2761 tcaaaaaaat agtgaaatga cttaccactt taaacagtgg ggggctggac attctgttag
     2821 tatttcagtg gagtctggtt cttttgttct aaaaccgtca gatcaatttg taggaaataa
     2881 acttgactta attttgaaac aagatgctga gggtaattac agatttgata gcagt
//
LOCUS       DAZPCS010000267         2932 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZPCS010000267 DAZPCS010000000
VERSION     DAZPCS010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.273"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..459)
                     /gene="intS"
                     /locus_tag="ACHUQ7_003968"
     CDS             complement(<1..459)
                     /gene="intS"
                     /locus_tag="ACHUQ7_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HFR1137139.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKSSSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGA"
     gene            complement(621..695)
                     /locus_tag="ACHUQ7_003969"
     tRNA            complement(621..695)
                     /locus_tag="ACHUQ7_003969"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(660..662),aa:Arg,seq:cct)
     gene            complement(769..1701)
                     /locus_tag="ACHUQ7_003970"
                     /pseudo
     CDS             complement(769..1701)
                     /locus_tag="ACHUQ7_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
     gene            1995..2750
                     /gene="mlaA"
                     /locus_tag="ACHUQ7_003971"
     CDS             1995..2750
                     /gene="mlaA"
                     /locus_tag="ACHUQ7_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFR1137140.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADALYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
BASE COUNT          718 a          707 c          700 g          807 t
ORIGIN      
        1 tgcaccgcga tcttcaaatc tgcggattac ttccagcagt tgcatcggct caatatcctg
       61 aatctcaagg ccgccgatga tgggtaaaat gtcgtcatca aacatttttg caagttcagt
      121 cgcataccct actgaccaga cttgcttctt gtgttcgtac cattccttgt aaatcgcact
      181 aaatgaattg ttgttagacg aagacttttt cgcctttacc ggatcgatgc cagccgagat
      241 gtctttcctc gcagtccatg ctttatccct tgcctcctgc aaagtcataa gcggatattt
      301 tccgacggtc aggattttct ccttaccgtc aatcttgtag cgaagctgcc ataccttttt
      361 cccggataca gggacataaa ggtacaggcc attaccatcg agaaggcggt atggtttttc
      421 tttcggcttt gctgcttcaa tctgcttaac ggtgagcatg ggtaaaaatc cggtgggtaa
      481 aattatttta tccacttttt acccgtcatg gagtgcggct gtcaacgatc tgacgcgaac
      541 catgacgaac tgtgaatata cggaaggctt gatattcagg ggattttgcg gactggtacg
      601 gatgggagcg aactgataaa tggtgtcccc tgcaggaatc gaacctgcaa ttagccctta
      661 ggaggggctc gttatatcca tttaactaag aggacaatgc ggcgagtata cccgctaatg
      721 gactgcgggg taagtacgct gccgctcgat tgcttaaacc ctcgccattt atgctgggtt
      781 tttatcattt ttcttaatgt tttccgcacg ttctgctttt tggcgtgctt ctgctttacg
      841 cttgttgctc atgtcgttac gaatctgtgc atgactcatt aacgcgaaga taaaggtgcc
      901 gccgcagatg ttccccgcta aagtaggtag tgcgaagggc cagatgaaat cgctccagtg
      961 cagcgtaccg ttaaacacca gatagaggat ttcaacagaa ccgaccacga tatgggtggt
     1021 gtcacccagg gcaataagcc aggtcatcaa tataatcacc acaatctttg ccgcacctgc
     1081 tgcaggaaac atccatacca tagtggcgat cagccagccg gaaatgatcg cgttggcaaa
     1141 catctcgctg ggggtgttct tcatcacatc catgccgatt ttgacaaatg catcgcgagt
     1201 ttcttcattg aagataggca tatattcaaa tgcccacgca gcaataccag tcccgagaat
     1261 attacccagc agcacgactc cccataaccg cataagtaag ccaacgttgc tcattgtcgg
     1321 tttttgcatg acgggtagta ccgcagtcac ggtgttttcg gtaaataatt gctggcgggc
     1381 cattatgacg ataataaaac caaaggtata accgagattc tccagcaaga agctgcccgg
     1441 cacaccttcc agttcggcat gaaatatccc ttttgccagt aacgaagcgc ccatcgacag
     1501 acccgccgca atggctgacc acagtagcgc cattgcgtcg cgttctagct ctttttcacc
     1561 atcctggcgg atatgctcat gaattgccat cgctcgggag gggagtcggt cttcatctat
     1621 ttctattttt ttgccgcgct ctttttcttc gctctaaact tcaatttcgt cgctgtgttg
     1681 atcaattttg tcgttgtcca tggtctcttc tcttgaatta gcacgtatag ctaaagcgta
     1741 gcggcttttt tgctcgcaac tggcgggagt tactctgaaa atgtagaaaa ggctgcgttt
     1801 gcctttttct gtttctatag aatcaagtag cctacagggc ggcgattacc aggctatgat
     1861 caaatcagca aatcagggcg tctggacatc agttgacgtg ctgttacaat cgcccacacc
     1921 taaacaggcg gatacggtat cgttccgtca tggatggcaa actgcataag ccataaaaaa
     1981 acagggagac atttatgaag cttcgcctgt cggcgcttgc tctgggaact acgcttctgg
     2041 tggggtgtgc gagttccggt acagatcagc aagggcgttc tgacccgtta gaagggttca
     2101 accgcaccat gtacaacttc aacttcaacg tattagaccc gtatattgtt cgaccggtcg
     2161 ctgtcgcctg gcgtgattat gttccgcaac cggcgcgtaa tggtttgagc aactttactg
     2221 gcaaccttga agaacctgcg gtgatggtta actacttctt gcagggcgac ccttatcagg
     2281 ggatggtcca ctttacccgc tttttcctga acaccatttt ggggatgggc ggctttattg
     2341 atgttgcagg gatggcgaac ccgaaactgc aacggactga acctcaccgc ttcggtagta
     2401 cgcttggtca ttatggcgtg ggttatgggc cttacgttca gttaccgttc tacggtagct
     2461 tcacgctgcg tgatgacggt ggtgatatgg cggatgctct ttacccggtt ctttcctggc
     2521 tgacctggcc gatgtctgtg ggtaaatgga cgcttgaagg gatcgaaacc cgcgctcagc
     2581 tgctggattc cgatggtctg ctgcgtcagt cgtccgatcc ttatattatg gtgcgcgaag
     2641 cgtacttcca gcgtcatgat ttcatcgcta atggcggcga actcaaaccg caggaaaatc
     2701 cgaacgcgca agcgattcag gatgatttaa aagatatcga ttctgaataa gaaacaaata
     2761 aaaaaggtga gtcgcaaaac tcaccttttt tgttgttatc cctcaattac cacaagtaaa
     2821 aggagataaa ccgacaatga cccggacgta atgatgtaaa aatactgatg tttgtcactg
     2881 actatttccg atattcaggc gttgaaaaat caaaaaactg gaaaaataat tt
//
LOCUS       DAZPCS010000268         2932 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZPCS010000268 DAZPCS010000000
VERSION     DAZPCS010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.274"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            75..650
                     /gene="ssuE"
                     /locus_tag="ACHUQ7_003972"
     CDS             75..650
                     /gene="ssuE"
                     /locus_tag="ACHUQ7_003972"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFR1137141.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQLIDYHHRPQFTPNLQTH
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            643..1602
                     /gene="ssuA"
                     /locus_tag="ACHUQ7_003973"
     CDS             643..1602
                     /gene="ssuA"
                     /locus_tag="ACHUQ7_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFR1137142.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1599..2744
                     /gene="ssuD"
                     /locus_tag="ACHUQ7_003974"
     CDS             1599..2744
                     /gene="ssuD"
                     /locus_tag="ACHUQ7_003974"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HFR1137143.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAY
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2756..>2932
                     /locus_tag="ACHUQ7_003975"
     CDS             2756..>2932
                     /locus_tag="ACHUQ7_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1137144.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELW"
BASE COUNT          659 a          800 c          806 g          667 t
ORIGIN      
        1 tgtctctccg acagactgac cgaattgtta tcaatgttaa caaaaaaaag aacaattggt
       61 tataaggaga gagtatgcgt gtcatcaccc tggcgggtag tcctcgcttt ccttctcgct
      121 ccagctcctt gctggaatat gcgcgggaaa aactaaatgg cctagatgta gaggtttatc
      181 actggaatct gcaaaacttc gccccggaag atctgcttta tgctcgtttc gatagtccgg
      241 cactcaagac cttcaccgaa cagctgcaac aggctgatgg gctgattgtc gccacgcctg
      301 tgtataaagc cgcctattcc ggcgcgctga aaaccctgct cgacctgctg ccagagcgcg
      361 ctttgcaagg caaagtggtg ctaccgctgg cgacgggcgg taccgtggcc catctgctgg
      421 cggtcgatta tgcccttaaa ccagttttaa gcgcactgaa agctcaggag atcctgcacg
      481 gcgtgtttgc cgatgactca caattaattg attaccatca cagaccccag ttcacgccaa
      541 atctgcaaac ccatcttgat accgcgctag aaactttctg gcaggcattg caccgccgcg
      601 atgttcaggt tcctgacctt ctgtctctgc gaggtaatgc ccatgcgtaa catcattaaa
      661 ctggcgctgg cgggattgct tagcgtctct acttttgcgg ttgctgcaga atcctcgcct
      721 gaagcgttac gtataggcta tcagaaaggc agtattggta tggtgctggc aaaaagccac
      781 cagttactgg aaaaacgcta tccgcaatca aaaatctcct gggtggagtt ccccgcgggt
      841 ccgcaaatgt tggaagcgtt aaacgttggc agtattgatc tcggcagtac cggggatatt
      901 ccgccaatct ttgcccaggc tgccggggct gatttggtat atgtgggcgt cgagccaccg
      961 aagcccaaag ccgaagtgat tctggtggca gaaaacagcc cgatcaaaac cgtagccgat
     1021 cttaaaggtc acaaagttgc ctttcagaaa ggttccagtt cacacaacct tttactgcgt
     1081 gcactgcgtc aggccggact taaattcacc gatatccagc ccacttacct gacgcctgcc
     1141 gatgcccgcg ccgcgttcca gcaaggtaac gttgacgcct gggctatctg ggatccctac
     1201 tactccgctg cgttattaca gggcggcgtg cgggtgctga aagacggcac cgatctcaat
     1261 caaactggat cgttttatct ggcagctcgt ccctatgcag aaaaaaacgg cgcttttatt
     1321 cagggcgtac tggcaacctt tagtgaggcc gatgcgttaa cccgcagcca gcgcgaacaa
     1381 agcatcgctt tactggcaaa aacgatgggc ttaccggcac cggtgattgc ctcttactta
     1441 gatcatcgcc ctcctaccac catcaaaccg gttaacgccg aggttgccgc cttacagcag
     1501 caaacggcag atctgtttta tgaaaaccgt ctggtgccga aaaaagtcga tattcgccag
     1561 cgcatctggc agcccactca actggaagga aaacaattat gagtctgaat atgttctggt
     1621 ttttaccgac ccacggtgac gggcactatc tgggaacgga agaaggttca cgcccggttg
     1681 atcacggtta tctgcaacaa attgcgcaag cggcggatcg tctgggctat accggtgtgc
     1741 tgatcccaac ggggcgatcg tgtgaagatg cgtggctggt tgcagcgtcg atgatcccgg
     1801 tgacgcagcg gctgaagttt cttgtcgccc tgcgtcccag cgtaacctca cctactgttg
     1861 ccgcccgcca ggccgccacg cttgaccgtc tctcaaatgg tcgtgcgttg tttaatctgg
     1921 tcacaggcag cgatccacaa gagctggcag gcgacggcgt gttccttgat catagcgagc
     1981 gctacgaagc ctcggcggaa tttacccagg tctggcggcg cttattgctg ggggaaaccg
     2041 tcaatttcaa cggtaaacat attcatgtgc gcggagcaaa actgctcttc ccgccgattc
     2101 aacagcctta tcctccgctt tactttggcg gatcgtcaga tgtcgcccag gagctggcgg
     2161 cagaacaagt tgatctctac ctcacctggg gcgaaccgcc ggaacttgtt aaagagaaaa
     2221 tcgaacaagt gcgggcgaaa gctgccgcgt atgggcgcaa aattcgtttc ggtattcgtc
     2281 tgcatgtgat tgttcgtgaa actaacgacg aagcgtggca ggccgccgag cggttaatct
     2341 cgcatcttga tgatgagact atcgccaaag cacaagccgc attcgcccgg acggattccg
     2401 taggacaaca gcgaatggcg gcgctacata acggcaagcg cgacaatctg gagatcagcc
     2461 ccaatttatg ggcgggcgtt ggcttagtgc gcggcggtgc cggaacggcg ctggtgggcg
     2521 atggtcctac ggtcgccgcg cgaatcaacg aatacgccgc gcttggcatc gacagctttg
     2581 tgctttcggg ctacccgcat ctggaagaag cgtatcgggt tggcgagttg ctgttcccac
     2641 atctggatgt cgccattccg gaaattcccc agccgcagcc gctgaatccg caaggcgaag
     2701 cggtggcaaa tgattttatc ccccgtaaag tcgcgcaaag ctaaggaaca cgatgatggc
     2761 aacgccagtg aagaagtggt tattgcgcgt tgccccctgg tttttaccgg tgggcatcgt
     2821 ggcggtgtgg caactggcct cctcggttgg ctggctgtcg acgcgtattt tgccttcacc
     2881 ggaaggagtg gtgacggcgt tctggacgct ctccgccagc ggcgaactgt gg
//
LOCUS       DAZPCS010000269         2906 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZPCS010000269 DAZPCS010000000
VERSION     DAZPCS010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.275"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..1541
                     /locus_tag="ACHUQ7_003976"
     CDS             <1..1541
                     /locus_tag="ACHUQ7_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFR1137145.1"
                     /translation="PTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVD
                     TYKIGIDKAIEAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGV
                     YSNTDAQDGIPLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFT
                     TFSAEEWQPQNRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVV
                     DRAKTLDQPFMLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKR
                     ILEQLKKNGQYDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKG
                     KLQPGNYDKLISAMDFYPTALDAADISIPKDLKLDGVPLLPWLQDKKQGEPHKNLTWI
                     TSYSHWFDEENIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVEN
                     NQLGLYKLTDLQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKAL
                     SEAK"
     gene            1593..2750
                     /locus_tag="ACHUQ7_003977"
     CDS             1593..2750
                     /locus_tag="ACHUQ7_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFR1137146.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNTLQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2716..2838)
                     /locus_tag="ACHUQ7_003978"
     CDS             complement(2716..2838)
                     /locus_tag="ACHUQ7_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1137147.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
BASE COUNT          883 a          632 c          586 g          805 t
ORIGIN      
        1 cgccgactga atacagtacc aaaggaaagc caaatattat cgtactaacc atggatgatc
       61 ttggttatgg acaacttcct tttgataagg gatcttttga cccaaaaaca atggaaaatc
      121 gtgaagttgt cgatacctac aaaataggga tagataaagc cattgaagct gcacaaaaat
      181 caacgccgac gctcctttca ttaatggatg aaggcgtacg ttttactaac ggctatgtgg
      241 cacacggtgt ttccggcccc tcccgtgccg caataatgac cggtcgagct cccgcccgct
      301 ttggtgtcta ttccaatacc gatgctcagg atggtattcc gctaacagaa actttcttgc
      361 ctgaattatt ccagaatcat ggttattaca ctgcagcagt aggtaaatgg cacttgtcaa
      421 aaatcagtaa tgtgccggta ccggaagata aacaaacgcg tgactatcat gacaacttca
      481 ccacattttc tgcggaagaa tggcaacctc aaaaccgtgg ctttgattac tttatgggat
      541 tccacgctgc aggaacggca tattacaact ccccttcact gttcaaaaat cgtgaacgtg
      601 tccccgcaaa aggttatatc agcgatcagt taaccgatga ggcaattggc gttgttgatc
      661 gtgccaaaac acttgaccag ccttttatgc tttacctggc ttataatgct ccgcacctac
      721 caaatgataa tcctgcaccg gatcaatatc agaagcaatt taataccggt agtcaaacag
      781 cagataacta ctacgcttcc gtttattctg ttgatcaggg tgtaaaacgc attctcgaac
      841 aactgaagaa aaacggacag tatgacaata caattattct ctttacctcc gataatggtg
      901 cggttatcga tggtcctctg ccgctgaacg gggcgcaaaa aggctataag agtcagacct
      961 atcctggcgg tactcacacc ccaatgttta tgtggtggaa aggaaaactt caacccggta
     1021 attatgacaa gctgatttcc gcaatggatt tctacccaac agctcttgat gcagccgata
     1081 tcagcattcc aaaagacctt aagctggatg gtgttccctt gctgccctgg ttgcaagata
     1141 agaaacaagg cgagccacat aaaaatctga cctggataac ctcttattct cactggtttg
     1201 atgaggaaaa tattccattc tgggataatt accacaaatt tgttcgccat cagtcagacg
     1261 attacccgca taaccccaac actgaggact taagccaatt ctcttatacg gtgagaaata
     1321 acgattattc gcttgtctat acagtagaaa acaatcagtt aggtctctac aaactgacgg
     1381 atctacagca aaaagataac cttgccgccg ccaatccgca ggtcgttaaa gagatgcaag
     1441 gcgtggtaag agagtttatc gacagcagcc agccaccgct tagcgaggta aatcaggaga
     1501 agtttaacaa tatcaagaaa gcactaagcg aagcgaaata actaaacctt catgcggcgg
     1561 atttttccgc cgccttattg agcgagatag cgatgcacgt tacagccaag ccctccagtt
     1621 ttcaatgtaa tctcaaatgt gattactgtt tttaccttga aaaagagtcg cagtttactc
     1681 atgaaaaatg gatggatgac agcactctga aagagttcat caaacaatat atcgaagcgt
     1741 ctggcaatca ggtctatttt acctggcaag gcggtgaacc cactctggct ggcctggatt
     1801 ttttccgtaa agttattcac tatcaacaac gctatgcagg ccaaaaacgt atttttaata
     1861 cattacaaac gaatggcatt ttattgaata atgaatggtg tgcctttctc aaagaacatg
     1921 aatttctggt tggtatctcg atcgatggcc cccaggagtt acatgaccgt tacagacgca
     1981 gtaattcagg taacggtact tttgcaaaag tgatagcagc catcgagcgt ctgaaatcat
     2041 atcaagtaga gtttaatacg ttaaccgtca ttaataacgt taatgtccat tatcctcttg
     2101 aggtttatca ttttttaaaa tctatcggca gtaaacatat gcaatttatc gaattgctag
     2161 aaaccgggac gccgaatatt gatttcagtg gtcatagtga gaacacattc cgtatcattg
     2221 atttttctgt gcctcccacg gcttatggca agtttatgtc aaccattttt atgcaatggg
     2281 ttaaaaacga tgtgggtgaa attttcattc gtcagtttga aagctttgtc agccgttttt
     2341 tggggaatgg gcatacaagt tgtattttcc aggagtcctg caaggataat ctggttgttg
     2401 aaagtaatgg agacatttac gaatgcgacc attttgtcta tccacagtac aaaattggaa
     2461 acattaataa atctgaactc aaaacgatga acagtgtaca actgacagcg caaaaaaaac
     2521 ggatttcagc gaaatgtcag caatgtgcat ataaacctat ctgcaatggc ggttgtccta
     2581 agcatcgtat tactaaagta aacaatgaga ctgtttctta tttttgcgaa ggttataaaa
     2641 tccttttttc aaccatggta ccttatatga acgccatggt tgagttagct aagaacagag
     2701 taccgcttta ccacattatg gatgttgcaa aacaaatgga gaataattaa taacgctctc
     2761 ttttaactcc attcttagcc tccttcttat gaccttaaca aggcacccga cataaattcc
     2821 tgtcgggtgt ttcgtcatca tcatcttccc ttgcgaaaat cctggcttac gtggtatgcg
     2881 tcagggcatt agcaataccc ttgatt
//
LOCUS       DAZPCS010000270         2898 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZPCS010000270 DAZPCS010000000
VERSION     DAZPCS010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.276"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..312)
                     /locus_tag="ACHUQ7_003979"
     CDS             complement(<1..312)
                     /locus_tag="ACHUQ7_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFR1137148.1"
                     /translation="MISNDILRSVRYILKANNNDLVRILALDNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DI"
     gene            complement(359..1078)
                     /gene="btsR"
                     /locus_tag="ACHUQ7_003980"
                     /gene_synonym="yehT"
     CDS             complement(359..1078)
                     /gene="btsR"
                     /locus_tag="ACHUQ7_003980"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFR1137149.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1075..2760)
                     /gene="btsS"
                     /locus_tag="ACHUQ7_003981"
     CDS             complement(1075..2760)
                     /gene="btsS"
                     /locus_tag="ACHUQ7_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFR1137150.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
BASE COUNT          750 a          774 c          658 g          716 t
ORIGIN      
        1 aatgtcatcg gttttcagcg aaaacgcgat gcgtaatttt ttcagcacga tgttgttatt
       61 aatgcgacgt tccggctcca gtgccggagc agactcatcc ttgccgcgtt tttcataaat
      121 caggccattg aggaatgacg acaaaacaat gtccggacaa cgctgaaaac cctcttcgtc
      181 ttctttacgt agccagacgg cgatctgttc cgcggtggct tcgacattat ccagcgccag
      241 aatacgcacc aggtcattat tattggcttt caaaatgtag cgcacgctgc gcagaatatc
      301 gttacttatc atgaggcctt caggtgttga tgaggcaaaa agccatttta gcagtctttt
      361 acaggccaat cgcctctttt aagcttttca gataacggcg gctgaccggc acggttaagc
      421 cattacgcaa aatcaactcg gcctggccgt tatcttccag acgaatctcc tgtaaatgcg
      481 cgaggttaac cagatactga cgatggcagc gcagtagtgg tgtacgactt tccaaggtac
      541 gtaatgtcaa ttcggtaaag ccctctttcc cttcgtggct ggtaacgtag acaccgctca
      601 tccgactgct gacaaatgcc acatctttca tttgcagcaa ataaatccga ctatgccccg
      661 tacaagggat aaatttcagc gcctgttgat tttccggtaa cagcgaaaca tcctgcttgc
      721 tgcgctcctg acgcaatcgc gccagcgttt tctccagtcg cgcttcatca attggcttca
      781 gcagataatc aaaggcatgt tcttcaaagg ctttaatagc gtattcgtca aacgcagtga
      841 gaaaaacaat atacgggcga tgttccgggt caagcatccc caccatttcc agaccactga
      901 tgcgcggcat ctggatatcg agaaacagca catccgggcg cagtttatgc accgcgccga
      961 tcccttccac ggcgtttgaa cactctccaa cgatttcaat atcgctctgc tcctgcaaaa
     1021 atatacgcag gttctcccgt gctaacggtt catcatcgac aattaagact ttaatcatgc
     1081 ctcgtccctc catggtagtc gtaacgttat tcgggtgtaa ctatcaggct cacaggcgac
     1141 gcttattcca tagtcatcgc caaaccgttc acgtaaacgc ttatccacca gattcatccc
     1201 cagcccactg gcattggtta ccggttgata caaaccggca ttgtcttcga tctccagcat
     1261 caaatgttgc ccctcacgtc gggcgctgat tgccactcgc cctgtatcca gcagttgtga
     1321 tgtcccatgt ttaatggcgt tttccactat cggttgcagg gtaaacgcgg gcaattgctg
     1381 ctgggataat tcttgcggaa tagcaatgtt gacctgcaac cgcgactgga agcgcgcctt
     1441 ttcaatttgc agataagcgt tcacatgttc aatttcgtcg gcgagagtaa caaactccga
     1501 aggccgcttt aagtttttgc ggaaaaaagt ggaaagatac tgcaccagct ggctggcctg
     1561 ttcgctgtcg cggcggatca ccgctttaat ggtgttaagc gcattaaaca aaaaatgagg
     1621 attcacctgg gcgtgaagca gtttgatttc tgactgggtg agcatcgctt tttgccgctc
     1681 atattgccct gcaagaatct gcgccgaaag cagttgcgca atcccctcgc ccagcgtgcg
     1741 gtttattgaa ctgaataacc ggtttttggc ttcatacaat ttgatggtgc ccatcacgcg
     1801 ctgattttcg ccacgcaacg gaattaccag cgtcgacccc agtttgcatt gcggatgcaa
     1861 agagcaacga taaggtactt cgttgccatc agcgtagacc acttcaccgg tttcaatcgc
     1921 ttttaaggtg taagtcgaag aaatcggttt gccgggtaaa tggtggtcgt caccaattcc
     1981 ggtaaaggcc agcaatttct ctcgatcggt aatcgcgact gcaccaatat ccagctcctg
     2041 atacagtacc tgagccactt tcatgctgtt cacttcgtta aacccctgtc gcaaaatgcc
     2101 ttccgtcgag gctgccactt tcagcgcagt ggcagaaaaa gccgaagtgt atttttcaaa
     2161 catcgcgcgt ttatcgagca atatacgcat aaacagcgcc gcgccgacgg tattggtgac
     2221 catcattggc gcagcaatat tactcaccag acgcaccgca tcttcataag gtcgggcgat
     2281 cgcaaggatg atcagcattt gcaccatttc agcgacgaac gtgacggcac cggcggtaat
     2341 ggggttaaag actttatcag tgcgcccgcg acggatcagg atgctgtgta ccaggccgcc
     2401 gagcaatcct tcaacgatgg ttgagatcat gcaacttagc gcggtcatgc cccccatcga
     2461 atatcgatgt aagccgccgg tcagaccaac cagcccaccg acgaccggac cgccgagtaa
     2521 gccgcccatt accgcgccta tcgcacgggt attggcaata gaatcgtcaa tgtgcaaccc
     2581 aaaccaggtg cccatgatgc agaagatgga aaagacgatg tagcagagaa atttatgcgg
     2641 cagacgaacc gtgacctgca ttaacggtat gaataatggc gttttactca ttaaccacgc
     2701 aatgactaaa aaaacgcaca tctgctgaag cagcagcaac accagattaa aatcgtacat
     2761 acccgcaaac cacacttccc tttaaaacgc gtaacataca ttgcctgcgt ttaactttct
     2821 ttgaactctt gcagaaaaat gagaattcgt gagtacgatc actcaaaatc gcctggcaaa
     2881 aataaaatca ccctatag
//
LOCUS       DAZPCS010000271         2891 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZPCS010000271 DAZPCS010000000
VERSION     DAZPCS010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.277"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..781
                     /gene="lsrF"
                     /locus_tag="ACHUQ7_003982"
     CDS             <1..781
                     /gene="lsrF"
                     /locus_tag="ACHUQ7_003982"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HFR1137151.1"
                     /translation="DWGMQSRLSWIFNPKTGKTVMLAFDHGYFQGPTIGLERIDINIA
                     PLFEHADVLMCTRGILRSVVPPATNKPVVLRASGANSILAELSNEAVALSMDDAVRLN
                     SCAVAAQVYIGSEYEHQSIKNIIQLVDAGMKVGMPTMAVTGVGKDMVRDQRYFSLATR
                     IAAEMGAQIIKTYYVEKGFERIVAGCPVPIVIAGGKKLPERETLEMCWQAIDQGASGV
                     DMGRNIFQSDHPVAMMKAVQAVVHHNETADRAYELYLSEKQ"
     gene            805..1095
                     /gene="lsrG"
                     /locus_tag="ACHUQ7_003983"
     CDS             805..1095
                     /gene="lsrG"
                     /locus_tag="ACHUQ7_003983"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HFR1137152.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            1135..1893
                     /gene="tam"
                     /locus_tag="ACHUQ7_003984"
     CDS             1135..1893
                     /gene="tam"
                     /locus_tag="ACHUQ7_003984"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HFR1137153.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(1897..2811)
                     /locus_tag="ACHUQ7_003985"
     CDS             complement(1897..2811)
                     /locus_tag="ACHUQ7_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HFR1137154.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTGDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
BASE COUNT          760 a          667 c          773 g          691 t
ORIGIN      
        1 ggattgggga atgcagtcac gcttatcgtg gatatttaat ccgaaaacgg gtaaaactgt
       61 gatgctggcc tttgaccatg gttattttca ggggccgact atcggacttg aacgcattga
      121 tataaatatc gccccgctgt ttgaacatgc cgatgtatta atgtgtacgc gcggcatttt
      181 gcgcagcgta gttcccccgg cgaccaataa gccggtggta ctgcgggcgt caggtgcgaa
      241 ctctattctg gcggaattaa gtaatgaagc cgtggcgtta tcgatggatg atgccgtgcg
      301 cctgaacagt tgcgcggtgg cggcgcaggt ttatatcggc agcgaatatg aacatcagtc
      361 gatcaaaaat attattcagc tggtcgatgc cggaatgaaa gtgggaatgc cgaccatggc
      421 cgtgactggc gtgggcaaag atatggtgcg cgatcagcgt tatttctcgc tcgcgactcg
      481 aatcgccgct gaaatggggg cgcaaattat caaaacctat tatgtcgaaa aaggttttga
      541 acggattgtt gccggatgtc cggtacccat tgttattgct ggcggtaaaa aattaccgga
      601 gcgcgagacg ctggaaatgt gctggcaggc tatcgatcag ggcgcttctg gtgtggatat
      661 ggggcgtaat attttccagt ctgaccatcc ggtggcgatg atgaaagccg tacaggcggt
      721 ggttcaccat aacgaaacgg ctgatcgggc atatgaactc tatctgagtg aaaaacagta
      781 actgcggatt taaggagaag aattatgcac gtcacactgg ttgaaattaa cgttcatgaa
      841 gacaaggttg acgagtttat cgaagttttt cgccagaacc acctgggctc tgtacaggaa
      901 gaaggtaatt tgcgcttcga tgtcttacag gacccggaag tgaattcgcg cttttatatt
      961 tacgaagcct ataaagatga agacacagtg gcattccata aaaccacgcc tcactacaaa
     1021 acctgtgtcg cgaaactgga atctttaatg accgggccgc gtaaaaaacg tctgttcaat
     1081 gggttgatgc cgtgaggcta caattggggt aacgcgctga cgggagtaaa aaaaatgtct
     1141 gactggaacc cctctttata tctgcacttt tccgctgaac gatcgcgtcc ggcggtggag
     1201 ctgcttgcca gagtgccgct ggagaatgtc gaatatgttg ccgatcttgg ctgtggccca
     1261 ggtaacagca ccgccctgct gcatcaacgt tggcctgcgg ccaggataac aggcatcgac
     1321 tcgtctccgg cgatgattgc tgaagcgcgc agtgctttgc cagactgcca gttcgtggaa
     1381 gcagacatcc gcaactggca accggaacag gcactcgatc tgatttttgc taatgcctca
     1441 ctgcaatggc tgcccgacca ctacgaattg tttcctcact tggtttcttt acttaatccg
     1501 cagggcgtgc tggcagtaca gatgccagat aactggctgg agccgaccca tgtgctcatg
     1561 cgcgaagttg cttgggaaca aaactacccg gatcgcgggc gtgagccgtt ggctggcgtt
     1621 catgcttact acgatatttt gagcgaagcc ggatgtgagg tcgatatctg gcgaaccact
     1681 tactatcacc agatgccgtc gcaccaggcg attatcgatt gggtgaccgc cactggatta
     1741 cgtccgtggt tacaggatct gaccgagagc gaacagcagc tttttcttac gcgctaccat
     1801 cagatgctgg aagagcagta tccactgcaa gagaacggac agatactgct ggcatttccg
     1861 cgtctgttta ttgttgcccg ccgtacggag taattatcac gtcagctggt aatgacgatc
     1921 gggaagaatt tttgctggaa tttctgcttc atcgttcatc tgtaacaggt caatttcaat
     1981 agcgttgcag atggcatcca gcggtaaatc attgttttca gtaccgaacg gatcttccag
     2041 ttcttccgcc agacaatcca gcgaaataaa agtgtaggaa atcagcacag agataaaagg
     2101 cgtcatgtaa tgcaggtcca cgaccagcgc gaacggcagc atgatacaaa acagataaac
     2161 ggtacgatgc aaaatcaggg tgtaggcaaa gggaattggc gtgtaggcaa tgcgctcaca
     2221 gcctgccaga accgctgaaa tatcattaag ccgatcgttg aggctaataa acaggatatc
     2281 tgaaagctgt ccattgcggc gctgaaccgc caaccattct cccattatta acaagatacg
     2341 gttagctgga gagttcgaag ccagtacacg ctgaagatct ccagtcttga gataatgagc
     2401 cagcacttcc gcctgtggct gtttgcgtaa tgtcatgcgt aaacagtggg cgaaggcgat
     2461 ttgcagcctg gcaaactccc ttacacttgc cgaatccggc aatgtcgttt ttacctctcg
     2521 cagtaacgac cgtgaggcaa tcatcaactg cccccaaagt tttcgcgctt caacgtaacg
     2581 ggcgtacccg gcattattac gaaaaccaag aaaaatggcg atggcgacac cgagaatgct
     2641 gaacggtgcg agggtgaatt tgatgcccag atgcgtgtac cagggcagca tgaatataac
     2701 agcaatagaa aagagaaaat tgagtagtaa gcgcgaggat atcttggata atactgagcc
     2761 gtgccagaca aaaatacggc gcagccagtg ttgttgtgga cgaacaatca tggttatctt
     2821 caggcgtgga aaagtcgccc tattaaacgt gattacgatc acattctcaa gacgctactt
     2881 acaaattacc t
//
LOCUS       DAZPCS010000272         2862 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZPCS010000272 DAZPCS010000000
VERSION     DAZPCS010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.278"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..647)
                     /gene="yidZ"
                     /locus_tag="ACHUQ7_003986"
     CDS             complement(<1..647)
                     /gene="yidZ"
                     /locus_tag="ACHUQ7_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFR1137155.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTQLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDT"
     gene            complement(622..1797)
                     /gene="mdtL"
                     /locus_tag="ACHUQ7_003987"
     CDS             complement(622..1797)
                     /gene="mdtL"
                     /locus_tag="ACHUQ7_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFR1137156.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            complement(1929..>2862)
                     /gene="tnaB"
                     /locus_tag="ACHUQ7_003988"
     CDS             complement(1929..>2862)
                     /gene="tnaB"
                     /locus_tag="ACHUQ7_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HFR1137157.1"
                     /translation="SANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAAS
                     RITSLFLGLKIISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFG
                     FHGNIPSLIICYGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGG
                     NIDSLVKSFLGTKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGR
                     FKTVLLTFLPPALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFT
                     VWGGNLIPAIVILFGITVILCWFGNVFNVLPKFG"
BASE COUNT          833 a          745 c          727 g          557 t
ORIGIN      
        1 caggtgtcgc tctgttccca gcaaatgctg atatgcggat aacgtaaaaa ggtgtccaga
       61 ttccacgttt catgcagtgc cggatgatct ttgcgtaacc agacgcaggg tacatcacta
      121 aacagcactt cataatcaat ggctaacggt agcgagctta acagctcccg cgagcgagga
      181 tggctttcgc gaccggaaaa accaatatcc acttcaccac gagtaatggc atctaaggaa
      241 tcgtaatccc agttacgtag tttgatggtc gcctgcgggt aacgttggta gatccgtttc
      301 gacagcgcat taagcatgat catcatcagc ggcgattccg ccgccaactc aaacttcagg
      361 ccgcgcggtg tctggtggtg cggtttatcc agcagcagat tgctcatttg catccattcc
      421 gccagatttt gctccatgct gaccattagc tgtgtgggcg acagccccag cggtgagttc
      481 acaaagagcg ggtcgtcaaa ccacgctctt aactttgcca gcgacttact caccgctgaa
      541 ggggtcacgt tcatccgctt cgccgctttg gtcacgctgc gctcctgcat cagcagttgc
      601 agacagagca gcagattgag atcaagcgtg gtgatggatt tcttcatgag cggcaacggg
      661 gcgtccaggc gcgacgaaca taatcagcaa caggctcact atgctacagg caatcagaat
      721 cccgatcagc atattccatg cgccgatacc aaccaccgcg gccagccaaa tccacagtga
      781 cgaaccgcaa acctgcgcaa tacctaaggt cgagctggct acgcccgcgc gtaatgaaaa
      841 tggtcctaac gcctgactca tcgctacgcc aaaacctacc gagaaaccgg cgcaaatcag
      901 cgtgatacca aacagagaaa ccgcatggga aggtgaaacg gcaagggtga tccccgccgc
      961 aaggaataac acctgcgagg tgatcatcaa cgtacgtggc ttaaaaattc ccagcgcaaa
     1021 tggcgtcgag aatgaaaagg tcatgctgac gccagcggtc agcgccataa tggtggcgta
     1081 ttctccgcgc tcaaacccca tgatttccat cagcaatacc ggcgatgtat tgacgaaagt
     1141 gaggatcacc gaaacgctga gggtggtgat aacaacacgg ctgaggaaaa aacgattaag
     1201 cagcgactcg ctattttctc gtggtttgtc cgaagcagcg ggggacgctg ggcgcgtttc
     1261 ttttaaaata aacaaagaca acatcagtac cgcgatgccc atcattgcca tcgcccagaa
     1321 cagactctgc cacgggaatt taagcataat cagatgtccg agcactggcg ctaacaccgg
     1381 aatgatgcag gtaataccgt tgagtaatga cagcacttta gcccgacgtc gatcatccag
     1441 cgtgtcgcgc aaaatggcga acgccaccac gtaacaacag cctgcgccca acccctgtag
     1501 aaatcggcct gcaagaaata acgtgctggt ttcagccagt gaacagaaca ccgaggcaat
     1561 aataaatagc gccgcgccgg gtatggcgac cggctttctc cctgaacgat cggccacttt
     1621 accggcaaat agcatcgcag ctgccatccc cgccagatat acggagaacg caatatgcaa
     1681 ctgcgcttcg ctggcattga gatcggcggc gatgcgcggt aaaccaacga ggtacatatc
     1741 aatcccggcg ggataaagta aaaccagggc aaaactacaa atcaaaaagc gggacatggg
     1801 ctaaagcgtc ctgatagtaa ggtgacgcaa agcatacgtg gtgaaggagg ataaggcgag
     1861 ttgccatttg gacataagtg atttcctgag aggcaagaag ccagcgaatg gctggcttct
     1921 tgaaggattt agccaaattt aggtaacacg ttaaagacgt tgccgaacca gcacaaaatc
     1981 acggttatac caaagagaat gacaatcgcc ggaataagat tgccgcccca gaccgtgaac
     2041 atctgattgg gaaacttctt gcgagctttg attgcaagca ctgcgggaat aatgaccgcc
     2101 cagatggtgg cgcacagccc ggcaccgccg atcccgtaaa taaagccgtt cgggaagatc
     2161 agatacaaca acgcaggtgg caggaaggtt aacagcacgg ttttgaaacg cccgccgtgg
     2221 gagttatcaa tcttaaacag gtccgccaga taatcgaaca accccagcgt gacaccaaag
     2281 aacgaactgg caacagctaa gttagagaac accagcaggc aaaactcgat aatgccgtgc
     2341 tgtttggtgc cgaggaacga tttcaccagc gaatcaatgt tgccgcctga ggagattatc
     2401 gccttaaagc tttcgcgcgg aatattcccc atcgtgcaat agagccagaa gagataaatc
     2461 accagcgcca gcagcgaacc aaataccacg cttttgatta acttatcttt gcgtttgcca
     2521 tagcaaataa tcaggctggg aatattgccg tggaaaccaa atgacgccag acacaccggc
     2581 aaagccataa agatatacgg gaagtaagac gttcccgcag tggtgctggt ggcatcgcgc
     2641 aggatggagt aatcgacctg gaagaagaaa gaaccaaaca cgatcacaaa ggagataatc
     2701 ttcagcccga ggaacaatga ggtaatgcga ctggcggcta acgaacttat ccacaatacg
     2761 ctggcaacga aaatggctgt gcagatcccg acaatacgtg gattagcgtg atagcccaaa
     2821 ttcattgata tcgtttcact aatgatcgca ccattagcag ag
//
LOCUS       DAZPCS010000273         2808 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZPCS010000273 DAZPCS010000000
VERSION     DAZPCS010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.279"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            6..81
                     /locus_tag="ACHUQ7_003989"
     tRNA            6..81
                     /locus_tag="ACHUQ7_003989"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="ACHUQ7_003990"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="ACHUQ7_003990"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(299..1057)
                     /locus_tag="ACHUQ7_003991"
     CDS             complement(299..1057)
                     /locus_tag="ACHUQ7_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFR1137158.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1065..2168)
                     /locus_tag="ACHUQ7_003992"
     CDS             complement(1065..2168)
                     /locus_tag="ACHUQ7_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFR1137159.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2178..>2808)
                     /locus_tag="ACHUQ7_003993"
     CDS             complement(2178..>2808)
                     /locus_tag="ACHUQ7_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFR1137160.1"
                     /translation="FILAVVMAIVLSVGLFRFNKTYQIKTGQLRRSWPIAAVLIIGLP
                     LLAQWLFGAALHWDVPALQGFNFRGGMVLIPELAALTLALSVYTSAFIAEIIRAGIQA
                     VPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSSLAAVIGYPDM
                     VSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVER"
BASE COUNT          763 a          740 c          675 g          630 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gttgcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaactg ccagacatca
      241 aatcaagcga aaggccatcc gaaaggatgg cctttttgct tttcgaacta acattcaatt
      301 aatggattac ctgcgataaa aatgccctcg tacgctctga tttaggatgc gcaaaaaatt
      361 catcaggtgt agcttgttcc actatctccc cacgatccat aaaaattacc cggtcagcga
      421 cggtacgtgc aaaccccatc tcatgtgtta cacacaacat tgtcataccc gactgcgcca
      481 gcccaatcat cgtatccagc acctctttca ccatctccgg atcgagcgcc gacgttggtt
      541 catcaaacaa cataattttc ggcttcatac acagcgaacg cgcaatggca acgcgttgct
      601 gctgaccacc tgaaatctgt ccgggaaact tatgcgcatg ttcggcaatt ctcacccgct
      661 caaggtaatg catcgccaga gcttcagcct ctttcttagg catcttgcgt acccaaatcg
      721 gtgccagggt acagttctgt aaaacggtca gatgagggaa gagattgaaa tgctgaaata
      781 ccattcccac ttcctgcctg acgcgctcaa tattgcggat atcttcatta agttcgatgc
      841 catctacgac aatccgtccc tgttgatgtt cttccagatg attaatacaa cgaatggttg
      901 tcgatttacc ggaaccagaa gggccacaca gaacgatccg ttctcccggt tgcacggtta
      961 aatttatatt tttcaaaaca tggaattgtc cataccattt atggacgttt tccagcgtaa
     1021 tcatcgcgtt agcaggttgc agtaaaattt ggctcatagt gtcctcaatg cggtgtacgc
     1081 ccggtgttaa aacgtttttc cagatgttgg ctataacgtg acatgctgaa acaaaagatc
     1141 cagtagatca gcgcggcgaa aacataccct tccgtcgaca tacccaacca ggcgggatca
     1201 acggttgcct gctgaacgct actgaaaaga tcgaacaagc cgatgatgat cactaggctg
     1261 gtatctttga agagtgcgat gatggtattt accagcccag gaattaccag cttcaacgcc
     1321 tgtggcagaa taaccagccc ctgagttttc cagtaaccca acgccagcga ctctgccgct
     1381 tcatattgcc ctttaggcag cgcctgtaat ccacctcgca cgacttccgc aacatatgct
     1441 gactgaaaca ggatcacgcc aaccagcgcg cggatcagtt tgtcgatact ggtgccttct
     1501 gccataaaca acggcagcat gaccgaagac ataaacaata cggtaatcag cggtacgccg
     1561 cgccagaact cgataaaaat gaccgataag atacgcacaa tcggcatatg ggagcggcga
     1621 cctaatgcca gtaagatccc ccacggtagc gcccccgcaa tcccaacgga tgcaataatt
     1681 aaagttagcg tcagcccgcc ccattgccgg gtttcaaccc gctcaagacc aaaaaaaccg
     1741 ccatacatca gccaccagac aatcagtggg taaatcaccg cccaggccgc aatatagcga
     1801 ccgcgatgcg ggagtatttt ccagaacatt ggtgcgatcg atacaagccc aatcagtaat
     1861 gcgaggttaa ttcgccagcg ttggtcgtgt gggtaaagtc catacataaa ctgaccaaat
     1921 cgttcgtgaa tgaagaccca acaggcaccg gcttttgtgc agtcagcacg cgttgagcca
     1981 acccaattag cctgcaaaaa tgcccagttc agcaacggtg ggatcagttc ccacatcaac
     2041 catatgcagc caatagtcag caggctattg ctccagctgg agaacagatt ttttcgcacc
     2101 cacaccatcg cgcggcttga gttatggctc gccgggcgcg agggctgaga cagcaatact
     2161 tttgtcatca tgactcctta gcgttcaacg atcgcgatgc ggcggttata gatattcatc
     2221 agcagcgaga tagtcaggct gataatcaga tagaccgaca tggtcatggc tatcgtctcg
     2281 atggcttgcc ccgtctggtt cagcacggtg ccagcaaaca gcgaaaccat atcgggatag
     2341 ccaataacgg cggcaagaga ggagtttttg acgatgttga gatactggct ggttaacggc
     2401 ggaataatta ctcgcagtgc ctggggaata atgacctggc gtagcgtaac cgggttgggt
     2461 aaccccagtg accgagccgc ttcatgttga ccataaggca ctgcctggat cccagcgcgg
     2521 ataatctcgg cgataaatgc agaggtataa accgaaagtg ccagcgttaa ggctgccagt
     2581 tcaggaatta aaaccattcc gccgcggaag ttaaagcctt gtagggctgg gacatcccag
     2641 tgtagtgctg cgccaaaaag ccattgcgcc agtaaaggca aaccaatgat caacactgcg
     2701 gcgatcggcc aggagcggcg cagttgtccg gtctttatct ggtatgtttt attaaagcgg
     2761 aatagcccaa cagaaaggac tatagccata acaacagcca ggataaac
//
LOCUS       DAZPCS010000274         2807 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZPCS010000274 DAZPCS010000000
VERSION     DAZPCS010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.280"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..141)
                     /gene="yjdI"
                     /locus_tag="ACHUQ7_003994"
     CDS             complement(<1..141)
                     /gene="yjdI"
                     /locus_tag="ACHUQ7_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFR1137161.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKS"
     gene            322..1953
                     /locus_tag="ACHUQ7_003995"
     CDS             322..1953
                     /locus_tag="ACHUQ7_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFR1137162.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            1950..2669
                     /gene="dcuR"
                     /locus_tag="ACHUQ7_003996"
     CDS             1950..2669
                     /gene="dcuR"
                     /locus_tag="ACHUQ7_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFR1137163.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
BASE COUNT          755 a          697 c          675 g          680 t
ORIGIN      
        1 cgactttcgt ttgagattaa ataacttgcc gttgccacgt acgcaattgc cagaatgctg
       61 acatatcgca gtgttgaaat agacatcgat cttttcgccg gtataacagc gataaccccc
      121 gtccagtagc gcctgatcca tgaccttccc tctctcatca gtatgattat cgctataagc
      181 atagccccgg aatcttcata cagcactggc agtttccgtt gccagagtct cttcccctat
      241 atagccaatc atttttactc cctttgaatt acccgcctca tcagagataa tgcttaagaa
      301 atttgtcaca caaggaagct gatgagacat tcattgccct accacatatt acgcaaacgt
      361 ccgatgaaat tgagtaccac tgtgatctta atggtcagcg cggtactgtt ctcggtgcta
      421 ttggtggtgc atctgattta cttctcgcaa atcagtgata tgacgcgaga cgggctggcc
      481 aacaaggcac tggcagtggc gcgtaccctc gccgactcgc cggaaatccg tcagggcttg
      541 cagaaaaaac cgcaggagag tggcatccag gccatcgcgg aagccgtgcg caaacgcaac
      601 gatctgctgt ttattgtcgt taccgatatg caaagtcttc gctactcaca tcctgaagcc
      661 cagcgtattg gtcagccatt taaaggtgat gacatcctta atgcgctgaa tggcgaagaa
      721 aatgtcgcta tcaatcgcgg ttttctggcg caggctttac gcgtatttac ccccatctac
      781 gatgaaaatc ataaacaaat tggcgtggtg gcgatcggcc ttgagttaag ccgcgtgacc
      841 caacagatca atgacagtcg ctggagcatt atctggtcgg tattatttgg catgctggtc
      901 ggactgattg gcacctgcat tctggttaag gtactgaaaa aaatcctttt cggcctggaa
      961 ccctacgaaa tctccacgct gtttgagcaa cgccaggcca tgttgcagtc tatcaaagaa
     1021 ggcgtcgttg ccgtggacga tcgcggcgag gtcacgctga tcaacgatgc cgcacaagaa
     1081 ttgctgaatt accgtaagtc gcaggacgat gagaaactgt cgacgctcag ccactcatgg
     1141 tcacaggtgg tagatgtctc ggaagtgtta cgcgacggta cgccgcgccg cgacgaagag
     1201 attacgatta aagaccggct attactgatc aacaccgttc cggtgcgcag taatggcgtt
     1261 atcatcggtg ccatttcaac cttcagggac aaaactgaag ttcgtaagct gatgcagcga
     1321 ctcgacggtc tggtcaacta tgctgacgca cttcgtgaac gatcccacga atttatgaat
     1381 aaattgcatg tgattctcgg attattgcat ctgaagagtt ataagcagtt ggaagattac
     1441 attctcaaaa cagccaataa ctatcaggaa gagattggct ctctgctggg taagatcaaa
     1501 tctccggtta tcgctggttt tttaatcagc aagattaacc gcgcgaccga tttaggccat
     1561 acgctgattt taaacagtga aagccagctg ccagacagcg gcagtgagga ccaggtcgcg
     1621 acgctgatta ccacattggg aaatctgata gaaaacgcgc tggaagcatt agggccggaa
     1681 cccggtggcg aaattagcgt aacattgcac taccgtcacg gctggctgca ctgcgaagtt
     1741 aacgatgatg gaccggggat cgcacccgac aaaatcgatc acatttttga caaaggtgtc
     1801 tcgacaaaag gaagcgagcg aggcgtcggt ttagcacttg tcaaacaaca ggtagaaaat
     1861 ctcggcggca gcatcgccgt ggaatcggaa ccggggattt tcacacaatt ttttgtccag
     1921 ataccctggg acggggagag gtcgaacaga tgatcaatgt attaattatc gatgacgacg
     1981 caatggtcgc ggagctgaat cgccgatacg tagcacaaat cccaggcttt caatgctgtg
     2041 gaacagcctc gacgctggag aaagccaaag agattatctt caatagcgat gcgcctatcg
     2101 acctgatatt gctcgatatc tatatgcaaa aagagaacgg gctcgattta ctgcctgtcc
     2161 tgcataacgc gcgttgcaaa agtgatgtga ttgtcatctc ctccgcagcc gatgcggcaa
     2221 ccattaaaga ttcgctgcat tacggtgtcg tggattacct gatcaaaccc ttccaggctt
     2281 cccgctttga agaggcgctc accggctggc ggcaaaagaa aatggcgctg gaaaaacatc
     2341 agtattatga ccaggccgag cttgatcagc taatccacgg cagcagctcc aacgaacagg
     2401 atcctcgtcg cttgccaaaa ggcttaacgc cgcagacgtt acgcacgctg tgccagtgga
     2461 ttgacgcgca tcaggactat gagttttcaa ccgacgagct ggctaacgag gttaacatct
     2521 cgcgtgtttc ctgccgaaaa tacctcatct ggctggtcaa ctgccacatc ttgttcacca
     2581 gtatccatta tggcgtcacg gggcgtccgg tttaccgtta ccgtattcag gcagaacact
     2641 actcactgct gaaacaatat tgccaataac ggatcgctgg ttatctgtaa gtaataatta
     2701 ttaccgtaat gcaaaatacc cctggcacaa gttacatcgt taatcagtaa aacttgtgcc
     2761 agatcaaata taattatccc tccatcatcg ttaaaaatta atatctc
//
LOCUS       DAZPCS010000275         2803 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZPCS010000275 DAZPCS010000000
VERSION     DAZPCS010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.281"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..98
                     /locus_tag="ACHUQ7_003997"
     CDS             <1..98
                     /locus_tag="ACHUQ7_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR1137164.1"
                     /translation="RLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            156..512
                     /locus_tag="ACHUQ7_003998"
     CDS             156..512
                     /locus_tag="ACHUQ7_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1137165.1"
                     /translation="MEIKPEDELSNIVLFPIKEDDPRNQVNFLYKPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
     gene            605..823
                     /locus_tag="ACHUQ7_003999"
     CDS             605..823
                     /locus_tag="ACHUQ7_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFR1137166.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPQIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            825..1190
                     /locus_tag="ACHUQ7_004000"
     CDS             825..1190
                     /locus_tag="ACHUQ7_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFR1137167.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPECHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEIYQEGATA"
     gene            1187..1852
                     /locus_tag="ACHUQ7_004001"
     CDS             1187..1852
                     /locus_tag="ACHUQ7_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1137168.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFYKAEVERWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            1852..2217
                     /locus_tag="ACHUQ7_004002"
     CDS             1852..2217
                     /locus_tag="ACHUQ7_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFR1137169.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVTLAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
     gene            2672..>2803
                     /gene="hokD"
                     /locus_tag="ACHUQ7_004003"
     CDS             2672..>2803
                     /gene="hokD"
                     /locus_tag="ACHUQ7_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR1137170.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     "
BASE COUNT          790 a          577 c          749 g          687 t
ORIGIN      
        1 ggcgcctgaa tataatctgt cgcgagtgcc ggaagagcga agctatgcag cgtgtactgg
       61 ctttctatca gggtaatttt caggaggcga tactgtgaat gaaattagct atcaggcttc
      121 aattaccgct ggcattcgca tcaaaggaga ggagcatgga aataaaacca gaagatgagt
      181 taagcaatat cgttttattt ccgataaaag aggatgaccc tcgtaatcag gttaattttc
      241 tttataagcc atcggaaaga ccatattgcc atcacgcctc tgtacgggtt gacgaaaaag
      301 agcgtcaggt ccgctgtaaa atctgcggtg cggttgtgga gccgtttgac tggatgctct
      361 ctgtggcaaa aagagaaacc agactggcag atgatgtaag gctattgcgc caggaggaac
      421 aggaaaggcg gagaaatata gaaaagctga tacagattga gcgtaacgcg aaagcgcgga
      481 tacgcagggc gacaaaatcc agaactgaat aattaaattt agcactgtta aaaatttaat
      541 ccttaaccgg agggatttct gcaccctcaa atcatcagga gaccgcccga aagggcggta
      601 atgaatggtt acattattta gaaaaaaata tccgcgaaag agtagaacaa cagaattcct
      661 gtttctcatt ctgtttatcg tgttgatgac accgatatcc ccgcaaattt ttgtctggat
      721 aatcggaaaa ataattgagc cagttattga attgtataac gacgtggtat gggcgtcatt
      781 caacacactg cacaataaaa ttaatccgta taaggaaagc tgatatggca ctgacgaaaa
      841 aacaacgtgc agaactgcgc atgaagttcg gtggtcgctg cgcttattgc ggctgcgaac
      901 ttggcgaaaa gtggcatgca gaccatgtaa aaccggtcat tcgttttgat ggaaatatgc
      961 ttcaccagga acgtgacgat atatccaaca tggtaccaga atgccaccca tgcaatctgc
     1021 acaagcattg cagtagcctg gaagattatc gacgaattat cagtgatggt cgtcgtgaat
     1081 tccttgcgtc cgggaaaggc aaagcgctgg ttcgtatggg attggttgaa atgaaatctg
     1141 acccggttgt gttctggttt gaaatatatc aagaaggggc tacggcatga ctactattac
     1201 caaagagcga ctgcaatggc tggctaacat ttctggccgc gatgatattg acgatataga
     1261 cggcggtgaa attcgtgaac tggcgcttat cgctctggca tcactggaag cagagcctgt
     1321 agcggaatgc attgttgaag atgggggcat gtgtattgat gggttcggtg agtatgtggg
     1381 tcactcgctg cctgatggaa tgcatgagct ttatgctgcc ccgcgaatgc gacaacctga
     1441 tggatggaaa gccgtagctg tagcgtggaa ggtgacgttt actcaagttg accaggaatc
     1501 taatacgttc actgctatat atttttacaa agcggaagtt gagcgttggg tacgactgca
     1561 taaagcatgt gattttcggg cagatataac accgctttac gcagcgccgg cagtgccggt
     1621 tgcagtaaac gacgacatgg cttacgcatt ccatcacgca ctgtcagatt catcgcttgg
     1681 ctctgatgaa atcgaagaaa ttaaaaccgg tttgcgtgct gcctttgcca acgtcactat
     1741 ccaaccagag ccgatagtgc cggatgaaat cgggccaaac gatagtaata cgtttgatta
     1801 tgttgatggc tggaacgcct gccgcgctgc catgcttaag ggagataaat aatgattaat
     1861 cgaaccaaac tggagcacat tctcgaatat gccaggcagc agaaatgtat tgggcaactt
     1921 tgtaaaattc caccaggaga tatggttgaa atcgtggaaa tggccatgcg taaggctggc
     1981 aactctccgg taactctggc tggctggata agctgtagtg atgcagttcc tgctgaatat
     2041 tgcgatgtga ttcttctcga tgatctcggg aatgtattcc ccggttcctg ggataaggtt
     2101 ttttgcccca ctcgtggcgg gaataagatg gcttttgtgg acaaagacgg cgttgaagta
     2161 gagagctcaa ctcactggat gccgctaccg gaaccaccgc aggaggtgaa tcaatgacct
     2221 ggcctgaggc attcaccacg gtaggaattg cgatggcggt ggcgctggtg gtgtattcga
     2281 tttgccgttg gggttaacaa acaaaaaccc cggattgatg gtccggggtt tttgaaggaa
     2341 acaaaacaga aacagcaatt gccgttacct gttgttacca tggcaagtaa acgtatctca
     2401 ggcgagcgca ttgcgccgtt ctgacgcaga taaattagcc tggatagaag gtgctggcaa
     2461 taaaaaatag cgttttctta tcggtatcgg taagattgct gcgggtgctt gaggctgtct
     2521 gcctcgggca tgccactgta aggcagacag agaaaagccc cagttaacat tacgcgtcct
     2581 gcaagacgct taacattaat ctgaggccca atctatgtct cacaaatgta ggttagcctc
     2641 ttacgtgccg aaaggcaagg agaagcaggc tatgaagcag caaaaggcga tgttaatcgc
     2701 cctgatcgtc atctgtttaa ccgtcatagt gacggcactg gtaacgagga aagacctctg
     2761 cgaggtacga atccgaaccg gccagacgga ggtcgctgtc ttc
//
LOCUS       DAZPCS010000276         2769 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZPCS010000276 DAZPCS010000000
VERSION     DAZPCS010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.282"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..749)
                     /gene="ipaJ"
                     /locus_tag="ACHUQ7_004004"
     CDS             complement(<1..749)
                     /gene="ipaJ"
                     /locus_tag="ACHUQ7_004004"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HFR1137171.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIG"
     gene            complement(946..1755)
                     /locus_tag="ACHUQ7_004005"
                     /pseudo
     CDS             complement(946..1755)
                     /locus_tag="ACHUQ7_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            2102..>2769
                     /gene="istA"
                     /locus_tag="ACHUQ7_004006"
     CDS             2102..>2769
                     /gene="istA"
                     /locus_tag="ACHUQ7_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100 family transposase"
                     /protein_id="HFR1137172.1"
                     /translation="MVTFETVMEIKILHKQGMSSRTIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRAFIR
                     SLSVPQEQEPAVRFETEPGRQMQVDWGTMRNGRSPLHVFVAVPGYSRMLYIEFTDNMR
                     YDTLETCHRNAFRFFGGVPREVLYDNMKTVVLQRDAYQTGQHRFHPSLWQFGKEMGFS
                     PRLCR"
BASE COUNT          760 a          705 c          519 g          785 t
ORIGIN      
        1 atacctattt tagtatatcc tataaagtta gaattacttc ttcttgcacc taattccata
       61 gttgaaaaac aagaataatt ttctcctaca gctggatcca tatagcttcc atcaggtcta
      121 cacaacaccc agtggaggcc gacaggaact ccaataaagg atacagcaac agcttccaaa
      181 actctttggt tagaagataa cacatctcgt tgatgtacta tattacatcc catgccaatt
      241 aataagtccc ttgcatcggg ataaataaaa ctaattactt gcgaaaaaat gttactttct
      301 tcaacgacat aagcattcaa tccaagaagc ctcgtagcca tgactatctt atcaggcata
      361 gaataaccgg catccgcgat attatctttg tgtattctcg gattataatt accactagtt
      421 attaagtata aatcccgctc acaacgatta tccaaatcaa gagaactctt ccttgtcatt
      481 tcggacatag accctttgta ttgtggtatt ttatcaaccc ctaattcctt tgcagcacag
      541 agtaaactgg cagctccaca agaattgtca tacatctgct tcctcgccaa aaaacaacct
      601 tgttcgttta ctctattctc atttaccctc acatcagtct gatgagatat catatattct
      661 cttggtatag ccccctgcaa taaaacccca tataacataa cacctgtatg aatacatccc
      721 cgtttgcaag gcttccgttg ttccgacatc gacgagtaat tgcagcacag tattgcatcc
      781 agcaatgcga actactatga gaattagctg accgggtcct tattccccga acgaacttta
      841 aatccaaaac ctcatactct tttccgtcgt catcacgtcc gtcaccataa caacctttca
      901 gcatccaccc ttttattaag agttaatgtc catcagcatc agcaattaat accgcctgat
      961 actgacatat ttttgtcgta tttacgtcca taatcggtta ttgtgataca ggtcacacca
     1021 gcttatttgc tattgttaca ataaccacct tggttgagag ctgttttttc aactttgtta
     1081 tcaactcccc caactccttg gtcagtaagg ctgccgaccg agcacagtga atagatagta
     1141 tccgcaggta ccaccatgct tacttaatac tcagaaggcg aagtgctctc caattaactc
     1201 tgccgcacta tgcttataag ctgcttttca atgccgtcta tcaacatgtc caattcattc
     1261 aatctgttgt actggtaatc caacgcagta atgatatatg caggtattct ttcatccact
     1321 ccagtaacgt ttcatgcaaa gcattaattt acgcgctacg gaacttcacc agttgactga
     1381 gcaaacgatg tgggaccagt actgcctgca gttcttctat tgttacagcg atttctttac
     1441 caaacagctg aaccaccatc aagataaaga tagcctgaac attcatcgca ccattttgtt
     1501 accaatggca aaagatttaa cgaaccgtgc ctgcatcagg cggacttttt atccctgctt
     1561 tgttagctcc ctaaccttgt acagagagcc tccataagct cccatatcaa gcagacatgg
     1621 ttcgcagtta ctgacataca ccagaaaact ctgacagcag actcacttat cctccacttc
     1681 actcatatcc ttattggtga gatgaacctg aatgacatgt tttgcgacat aaacgccaac
     1741 cagtgtatat ttcacctgta gagattctac aatctgtagc gttatgcatc taatgttagg
     1801 ctctatgatg tcagaaatct gcgagactct acaccttctg catttacctt tatttatcac
     1861 catgctcctc agttaacgga gaacgtgttc attgcatttt gtcacttaca tttcgccttt
     1921 ggtatcgggg ggatataggt tccaacgttc cgcatgagaa cctgaaccgg cttcacgcca
     1981 ccttgatacc agatgccgca caggtagtca acaagtgtca cgaacggtgc aatagtgatc
     2041 cacacccaac gcctgaaatc agatccaggg ggtaatctgc tctcctgatt caggagagct
     2101 tatggtcact tttgagacag ttatggaaat taaaatcctg cacaagcagg gaatgagtag
     2161 ccggacgatt gccagagaac tggggctctc ccgcaatacc gttaaacgtt atttgcaggc
     2221 aaaatctgag ccgccaaaat atacgccgcg acctgctgtt gcttcactcc tggatgaata
     2281 ccgggattat attcgtcaac gcatcgccga tgctcatcct tacaaaatcc cggcaacggt
     2341 aatcgctcgt gagatcagag accagggata tcgtggcgga atgaccattc tcagggcatt
     2401 cattcgttct ctctcggttc ctcaggagca ggagcctgcc gttcggttcg aaactgaacc
     2461 cggacgacag atgcaggttg actggggcac tatgcgtaat ggtcgctcac cgcttcacgt
     2521 gttcgttgct gttcccggat acagccgaat gttgtacatc gaattcactg acaatatgcg
     2581 ttatgacacg ctggagacct gccatcgtaa tgcgttccgc ttctttggtg gtgtgccgcg
     2641 cgaagtgttg tatgacaata tgaaaactgt ggttctgcaa cgtgacgcat atcagaccgg
     2701 tcagcaccgg ttccatcctt cgctgtggca gttcggcaag gagatgggct tctctccccg
     2761 actgtgtcg
//
LOCUS       DAZPCS010000277         2768 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZPCS010000277 DAZPCS010000000
VERSION     DAZPCS010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.283"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            421..564
                     /locus_tag="ACHUQ7_004007"
     CDS             421..564
                     /locus_tag="ACHUQ7_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFR1137173.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            577..786
                     /gene="cspE"
                     /locus_tag="ACHUQ7_004008"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             577..786
                     /gene="cspE"
                     /locus_tag="ACHUQ7_004008"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFR1137174.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            952..1761
                     /gene="rlmA"
                     /locus_tag="ACHUQ7_004009"
     CDS             952..1761
                     /gene="rlmA"
                     /locus_tag="ACHUQ7_004009"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFR1137175.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1758..2324)
                     /gene="mntP"
                     /locus_tag="ACHUQ7_004010"
     CDS             complement(1758..2324)
                     /gene="mntP"
                     /locus_tag="ACHUQ7_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFR1137176.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT          757 a          632 c          646 g          733 t
ORIGIN      
        1 ttgctcactc atcaattttt cttattttaa atttacaatc cctttgggga ttgacttctc
       61 tttagggtaa ttgatagccg ttaactgact gttttatgag aaaaagtgat ataacttttt
      121 attcattgca tagcagaaaa tgtgatattg cacgcactat gtaataactt ctccctctgg
      181 cctggaacaa ctgaacttat tgaactatgt tagaaactac gccagtttaa gtatctgcct
      241 gaactggcaa ggttaagcac aatgatatat cggcgcgtat tccgttgcat aagtgtgcaa
      301 aaaaagtgga agacgtatcg agatttgtgc gtctgatcga gatatgttta aaaatggctt
      361 gccataatta acgttgtatg tgataaaaga tttcgggtta aacgaggtac agttctgttt
      421 atgtgtggca ttttcagtaa agaagtcctg agtaaacacg ttgacgttga ataccgcttc
      481 tctgccgagc cttatattgg tgcctcatgc agtaatgtgt cagttttatc tatgttatgc
      541 ctgcgggcga agaaaacaat ctaaggaatt tttcaaatgg caaagattaa aggtcaggtt
      601 aagtggttca acgagtctaa aggttttggc ttcattactc cggctgatgg cagcaaagat
      661 gtgttcgtac acttctccgc tatccagggt aatggcttca aaactctggc tgaaggtcag
      721 aacgttgagt tcgaaattca ggacggccag aaaggtccgg cagctgttaa cgtaacagct
      781 atctgatcga atccactgat ctgaagtgtg aatacgcttc aatctcgcta taaagcctcg
      841 tcgaatgcga ggctttttac tatgctttat cttcgctcct ggcgttcgga tatttgcccg
      901 ccgcgtgatt cgcgttacac ttgcggcctt tagtatcctg ccggagttgt catgtcgttt
      961 tcctgtccac tttgccatca gcctctttcg cgtgaaaaaa acagctatat ctgtccccaa
     1021 cgacatcagt ttgatatggc gaaagaaggg tatgtcaatc tgctgcccgt tcagcataaa
     1081 cggtctcgtg atccgggcga cagcgcggaa atgatgcaag cacgccgcgc attcttagat
     1141 gccggacatt atcagccgct gcgtgatgca attgtcgccc aactgcggga acggcttgat
     1201 gaaaaggcca cggcggtgct ggatattggc tgtggtgaag ggtattacac acacgcattt
     1261 gccgatgcgt tgcccgaaat caccacgttt ggcctggatg tctcgaaggt agcgataaaa
     1321 gcggcggcga aacgctatcc gcaggtcact ctttgtgtcg cttccagcca ccgtttgccg
     1381 ttttccgata ccagtatgga cgccataata cgtatttacg cgccgtgtaa agcagaagaa
     1441 ttagcacgag tagtgaagcc cggcggctgg gtcattactg ccacgccggg accgcgacat
     1501 ttgatggagc tgaaggggct gatttacaat gaagtacatc ttcatgcacc tcatgcagaa
     1561 caactggaag gttttacatt acagcagagt gcggagttgt gttatccgat gcgtcttcgc
     1621 ggtgatgaag ccgtcgcatt attgcagatg acgccgtttg cctggcgtgc gaagccagaa
     1681 gtctggcaaa cactggcagc aaaagaagtg ttcgactgcc agacggactt taatattcac
     1741 ctctggcagc gttcttatta accgtggaag tgcgtccaga ggatctggac gccgatgccg
     1801 atcagcacca gcccgccgag aatttccgct tttttcccaa taattgagcc gataaagcga
     1861 ccaaccatca tccctaatgt cgacataatc aaggttgcac aaccaatggc caatgcggtc
     1921 gcgataatgt tgacctgaag gaaagcaaga ccaacaccca cagccatggc atccaggctg
     1981 gtggcaatcg cggtggttac cagtagccag aaaccgtgcc gacggcgcgg ctcttcatct
     2041 tcatcatctg cgccacgaaa accctcaata atcattcgcc cgccgaggaa tatcagcagc
     2101 acaaacgcaa tccagtgatt ccattcaagg acaaaccggc tggctaacat gcccattccc
     2161 cagccgatca gcggcgtcag ggtttcgacg gcaccaaaaa taaggccggt tcgcaatgct
     2221 tcagaaaatt tcggtttatg gaggggggca cctttaccga ttgatgcagc aaatgcatcc
     2281 atcgacatac caaacgcaag aagaacagta gcagtgatat tcatgacaac gtcctgaccg
     2341 gggtatccat aaaacacatc atcgccccca gcaaacatgc atacatgaac aacaagccgc
     2401 gatgggtttt cgcgttcatg caggcatagc gattgatgtg catatggtct cgcctgactg
     2461 aaagtcttca gtccgtacgt gccacgtttt gcaacgagta tgttgacacg tacatttccg
     2521 gaatctggaa atcggctact ccccaaatga gggcgcaacc ttaacatatt ttgagaatat
     2581 aaaacaactg ttggaatata ttatttcatt gctttattga taacgatttt catttatgtt
     2641 tatatatcaa tgaaacgtta aaataaataa caaataaggg aagagataat atagcctaag
     2701 ctatgtctga ttattaaaaa tacactaaac atagctttgg ctaaattcat aagttattga
     2761 gttgatac
//
LOCUS       DAZPCS010000278         2736 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZPCS010000278 DAZPCS010000000
VERSION     DAZPCS010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.284"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            90..155
                     /locus_tag="ACHUQ7_004011"
                     /pseudo
     CDS             90..155
                     /locus_tag="ACHUQ7_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            383..631
                     /locus_tag="ACHUQ7_004012"
     CDS             383..631
                     /locus_tag="ACHUQ7_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFR1137177.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(747..1016)
                     /locus_tag="ACHUQ7_004013"
     CDS             complement(747..1016)
                     /locus_tag="ACHUQ7_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR1137178.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQGFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
     gene            1073..1741
                     /locus_tag="ACHUQ7_004014"
     CDS             1073..1741
                     /locus_tag="ACHUQ7_004014"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR1137179.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            2057..>2736
                     /locus_tag="ACHUQ7_004015"
     CDS             2057..>2736
                     /locus_tag="ACHUQ7_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR1137180.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTL"
BASE COUNT          835 a          590 c          538 g          773 t
ORIGIN      
        1 ctatagatgc acaaaaaacg ggcaaaacta cctggttcgc aaaactgcgt ctaaagttaa
       61 actgggacct cgcgagcaag ggtgagacga tggcgcttta cacaattggt gaagtggcgt
      121 tgctttgtga tattaatcct gtcacgttac gcgcgtgacg caacattttt aaaaatcgac
      181 gaagatcgtc caggagcgcc gtttttcaag ggttggatag atgattcgtt taccatgtcg
      241 acgaacttcg acgaatttcg ccgataaaaa gtgtcccata catgccccat tgatgcccca
      301 taaacaatat caggaattga cactggttat ccatacagat aaaaataaca cctgtacata
      361 actacagtta caggagggtg ttatgcgtgt tgagatctgt atcgctaaag aaaaaatcac
      421 taaaatgcca aacggtgctg tggatgcgtt aaaggaagaa ttaacccgac gcatcagtaa
      481 acgttatgac gatgtagagg tgatcgtaaa agccaccagc aacgatggcc tttctgttac
      541 acgcaccgca gataaggatt ctgcaaaaac atttgttcag gagactctga aagatacctg
      601 ggagtctgct gacgagtggt ttgttcacta ataaacacgt aaaatcggta acggctggaa
      661 atcattcaat actcgcacta tcgaaagttt accagccaac cgcagcacgt cctgcatacg
      721 tcgtgtctgc ggtttttctt tttcgcttac attgtgtctg gttcttccgg ccactcaata
      781 tcaggtgcag ttgatgtatc aacacggttc agcaacaccc gatacttctt ccaggcttcc
      841 agcaacgagt tttcttcctc cgttgcgatc tccagatcta cagcatcctg aagtggcgca
      901 atatgctcac tgaatccctg gatgtagaac tgtgtggtga cggtcttcca gccattcggc
      961 tcctgctgta tcgaagcata ccaggctatt tcaatatcgc tatgctgcgg cagcatttaa
     1021 ccccttgtaa ttcatcgcca taattgattt aattcacaaa taaaactata atatggtgaa
     1081 attaatgaaa aaaaacacag atgatggggc taaaatttac acaccactta ccctaaagct
     1141 ttatgactgg tgggttttgg gagtatcaaa tcggcttgca tggggatgtc ctacaaagga
     1201 acaccttctt ccacactttc tggaacattt aggtaacaac catctggata ttggtgttgg
     1261 aactgggttt taccttactc acgtacctga gagtagtctg atatctttaa tggatttgaa
     1321 cgaagctagc ctgaacgcgg catctacaag ggctggggaa tcaaaaatta aacataaaat
     1381 tagccatgat gtttttgaac cttatcctgc ggcgttacat ggtcaatttg attccatttc
     1441 catgttttac cttcttcact gcctgcctgg aaatatatct acaaaaagct gtgtaatacg
     1501 caatgcggcg caggccttaa ctgatgatgg aactctatac ggagccacaa ttcttggcga
     1561 tggagttgtg cacaatagct tcggtcaaaa actgatgcgc atttacaatc agaaaggcat
     1621 cttttcaaac acaaaagatt ccgaagaagg cttaacacat atactctcag agcatttcga
     1681 gaatgttaaa accaaggttc aaggtactgt agtaatgttt tccgcttcag ggaaaaaata
     1741 gcatccaacc gcagcacgtt cttgcttaag acgtgctacg gtttccttta cgatttgggg
     1801 ttgcgacttt acctctattg ataatgcatt ccggcagaac gttcaaatat gaacgtacga
     1861 tatttaacta accgaaaaac aaaatatagc acaggcgaga catttaccat ctaagaaaat
     1921 agtatcgttt ttttacagtt aaatcaacat cacttcctta aaatgaaaac aataaatttc
     1981 aatcctgaaa cattttattt cagcttatta ttccccagta tcacatttta tcaggatatc
     2041 cttctgcgag gttattatgc ttcctgtaaa taatcccccc ctatccactg gaaacgtctc
     2101 tttttacaga actacatcaa tcgacaatgt tcacaataat tatctctccg aatgggttga
     2161 atggactaaa aacagcattt ccggagaaaa cagggaaact gcttttaccc ggctccaatt
     2221 atgtctggag aacagtgaaa catcgttgga cttatcttgt ttaggtctca gatctctacc
     2281 acgattgcct gacaatcttg atgaaattaa tgtaagcaat aaccaactat caatgctccc
     2341 cgagctacca agggcattga aagagctgaa tgcaagcagt aatcaattat ctgcacttcc
     2401 tgaattacca gtgtcgctgg aatatataaa tgtgagtgat aaccatttgt tcgcacttcc
     2461 tgaattacct gcgtcactag aatatattaa tgtaagtgac aatcacctgt ctgtacttcc
     2521 gaggttacca atgtcattgg aattacttga tgcagccaga aatgctttgg aagtaatacc
     2581 agattttcca gaaagagatg atcatattat aagaatattc tggcttaatc agaaccggat
     2641 cacggcaatt ccggaaagca tacttggcct cagttctgat agcgttgtca atcttagaga
     2701 aaatcaacta tctcccagaa taatgcaaac tttgtt
//
LOCUS       DAZPCS010000279         2721 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZPCS010000279 DAZPCS010000000
VERSION     DAZPCS010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.285"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..357
                     /locus_tag="ACHUQ7_004016"
     CDS             <1..357
                     /locus_tag="ACHUQ7_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011579066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFR1137181.1"
                     /translation="VFVHTKPAYRSDGTKTAAVRKMRTDSNKAWKGALKRAGISNFRF
                     HDLRHTWASWLVQSGVSLLALKEMGGWETLEMVQRYAHLSAGHLTEHASKIDAIISRN
                     GTNTAQEENVVYLNAR"
     gene            619..981
                     /locus_tag="ACHUQ7_004017"
     CDS             619..981
                     /locus_tag="ACHUQ7_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFR1137182.1"
                     /translation="MLKLFVKYTSIGVLNTLIHWVVFGVCIYAAHTSQALANFTGFVV
                     AVSFSFFANARFTFKALTTAMRYMLYVGFMGILSVIVGWAADKCSLPPIVTLITFSAI
                     SLVCGFVYSKFIVFRDAK"
     gene            978..1907
                     /locus_tag="ACHUQ7_004018"
     CDS             978..1907
                     /locus_tag="ACHUQ7_004018"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFR1137183.1"
                     /translation="MKISLVVPVFNEEEAIPVFYKTVREFQELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHASGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGPTDVVEYVRAERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTLVFGNPVRGYPSLLVSILFLGGVQL
                     IGIGVLGEYIGRIYIEVKNRPKYIIKKSHRGNP"
     gene            1904..>2721
                     /gene="gtrII"
                     /locus_tag="ACHUQ7_004019"
     CDS             1904..>2721
                     /gene="gtrII"
                     /locus_tag="ACHUQ7_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase GtrII"
                     /protein_id="HFR1137184.1"
                     /translation="MIKINLFKNANLLAFISCFAISIYCYWGWLYDGTLNIDGEFTNN
                     FYQTITLGRWFHTFLRHYFLPEPFSLYITPLIALSFIIISAFIICRSLKLESYELLIG
                     MLVFITFPQISYQLEFLNQADTVGIAFLLAAISAIIFHSQKNRIVIFSGIVLSILSMA
                     IYQTFVTYIIAFVIGLQINSIIRNEKNIRESFYSSCLSLSLIALSTLIYLLLTKAIKH
                     YFSLESNEYISNYIQNASDIKWLVKSAIDNIYNFYNNPPAGLNLYKWLLIPLLIL"
BASE COUNT          787 a          540 c          554 g          840 t
ORIGIN      
        1 gtgtttgtcc acaccaaacc tgcctaccga agcgacggaa caaaaacagc agcggtaagg
       61 aagatgagaa ccgacagcaa caaggcatgg aagggagcgt taaagcgggc aggcattagc
      121 aacttccgct tccatgacct gaggcatacc tgggcaagct ggctggttca gtccggtgtc
      181 tctcttcttg cgcttaaaga gatgggagga tgggaaactc tcgaaatggt tcaaagatac
      241 gcccaccttt cagccgggca tcttaccgag cacgcgagca aaatcgatgc gattataagt
      301 cgcaatggca caaatacggc acaagaggag aacgtagttt acttaaatgc gaggtaactt
      361 attgatttaa atggtgccga taataggagt cgaacctacg accttcgcat tacgaattat
      421 aagaatccgc ttgtaattca aaggattatc ccatcaacac tgcgctcaca cgtcccacca
      481 catcaaaaca tgtaaagcct tgcaagccat tgtgaggcct tatgtgtctc agttttgtct
      541 catcagacat agcaagtatc gatcaattga gacttggatg atagacttca tgcctttcag
      601 agctcattga ttaaataaat gttaaagtta tttgtaaagt acacatctat aggtgtgctg
      661 aacacactta tacactgggt ggtttttggt gtatgtatct atgccgcaca taccagtcag
      721 gctctggcaa acttcacagg tttcgtagtg gctgtgagct ttagcttctt cgcgaatgca
      781 agattcacat tcaaagcatt gactacagcg atgcgctaca tgttatacgt gggatttatg
      841 ggaatattga gtgtgattgt tggttgggca gctgataaat gctcacttcc tccaatagtc
      901 actcttatca ccttctccgc catcagtctg gtgtgcggtt tcgtctattc aaagttcatt
      961 gtctttaggg atgcgaaatg aaaatatctc ttgtcgttcc tgtcttcaat gaagaagaag
     1021 cgatacctgt tttctataaa acggtacgtg aattccaaga gttgaagcca tatgaagtag
     1081 aaattgtatt cataaatgac ggaagtaaag atgccacaga gtcaattatt aacgcgctgg
     1141 ctgtttcaga cccgctagtt gttccgctgt catttacacg caactttggt aaagaaccag
     1201 ccttatttgc agggttagac catgcaagcg gcgatgctgt aattcctatt gatgtcgacc
     1261 tgcaagaccc aattgaggtt atccctcatc ttattgaaaa gtggcaggca ggtgctgaca
     1321 tggtgcttgc taaacgttca gaccgctcaa ctgatggacg actgaaacgt aagacagctg
     1381 agtggttcta taaattacac aacaaaataa gcaccccaaa gatcgaggaa aatgtcggag
     1441 attttcgact catgtctcgt gaggttgtgg agaacattaa actgttgcct gagcgcaatc
     1501 ttttcatgaa aggcatactg agctgggtgg gtggtccgac ggatgtcgtt gaatatgtac
     1561 gcgcagagcg tgttgctggc atctcaaaat ttaatggctg gaaattatgg aatctggcac
     1621 tggaaggtat cacaagtttt tcaacctttc ctcttcgcgt atggacttat ataggcttgt
     1681 ttgttgcaag catttcattt ttatatggtg catggatgat tatagacacc cttgtctttg
     1741 gtaacccagt acgcgggtat ccctccctgc ttgtatcaat acttttcttg ggtggagtgc
     1801 aactgatcgg gattggtgtt ctcggagaat atataggtag aatctatatt gaagtaaaaa
     1861 atagacccaa atacatcata aaaaaatctc atcgaggtaa cccatgatta aaattaattt
     1921 atttaaaaat gcaaatctcc ttgccttcat atcatgtttt gcaatatcaa tttattgtta
     1981 ttgggggtgg ttgtacgatg gaacacttaa tattgatggc gagtttacaa ataattttta
     2041 tcaaacaatc acgcttgggc ggtggtttca cacttttttg cgacattact ttctccctga
     2101 gcctttttca ctttatataa caccattaat agccttatct tttatcatca tttcagcatt
     2161 tataatctgc agatcgctaa agctagaatc ttatgaatta ttgataggta tgttagtatt
     2221 tattaccttc cctcagatct cctatcaatt agagtttctt aaccaagctg atactgtggg
     2281 aattgctttt ctactggcag cgatatcagc aattattttt cactcgcaaa aaaataggat
     2341 tgtgatattt tctggtatag tactgtccat tctttcaatg gcaatctacc aaacattcgt
     2401 aacatatatt attgcattcg tcattgggtt gcagataaat tcgataatac gaaatgagaa
     2461 aaatattcgt gaatcttttt atagttcatg tttatctcta tccctcatag ctttatctac
     2521 cttaatttac ctgctattaa ccaaagctat caagcattat ttttcgcttg aatcgaacga
     2581 gtacatctca aattatatac aaaatgcaag cgatattaaa tggcttgtta aatcagccat
     2641 agataatata tataacttct ataacaatcc tcccgctggt ttaaacctat acaagtggtt
     2701 actgattcct ttattaattc t
//
LOCUS       DAZPCS010000280         2714 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZPCS010000280 DAZPCS010000000
VERSION     DAZPCS010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.286"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            52..2574
                     /gene="lpfC-O113"
                     /locus_tag="ACHUQ7_004020"
     CDS             52..2574
                     /gene="lpfC-O113"
                     /locus_tag="ACHUQ7_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial usher protein LpfC-O113"
                     /protein_id="HFR1137185.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEINHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIHQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGLTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKLFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAARLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSFSTPESESGLIEQGVTCH"
     gene            2585..>2714
                     /locus_tag="ACHUQ7_004021"
     CDS             2585..>2714
                     /locus_tag="ACHUQ7_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1137186.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFS"
BASE COUNT          727 a          585 c          679 g          723 t
ORIGIN      
        1 agtgacccga taaaaatgac gctgtaagca agggataagg ataggcgtat tatgatgacg
       61 accagaatag tggttggcct cacggcaggg acgtgtctga ttttctcgca aaacctgatg
      121 gccgaggtca gtgtattcaa tccggcgctt ctggaaatca accatcaatc cggagtcgat
      181 attcgccagt ttaatcgggc aaacctgatg cccccaggtg tttatagcgt tgatattttt
      241 atcaacggta aaatgtttga acgtcaggat gtgacatttg ttcaggataa tccagatgct
      301 gatctgcacg cttgctttat tgccattaaa aaaacactgt cctcctttgg cataaaagtt
      361 gatgcgctca aatcgttcaa tgatgtggat gagacggttt gcctcgatcc tgctccacgt
      421 attgaaggct catcctggca gtttgacagt gataaattgc agctgaatat atccattcac
      481 caaatctaca tggacgcgat ggcttatgat tacatcagcc ccacgcgttg ggatgagggg
      541 attaatgcgc tcaccatcaa ctacgatttt tctggttcac atacactacg ttcagattat
      601 ggttcacaag agacagatac cagttatctc aatctgcgca atggactgaa tattggaccg
      661 tggcggctac gtaattacag tactttaaac accagcgatg gccgtgcgga atacaactcc
      721 attagtacct ggatacagcg cgatattgcc gcgttaagaa gccagattat gattggtgat
      781 acgtggacgg cgagcgatat tttcgacagt acgcaaattc gcggcgcgcg tttgtatact
      841 gataacgata tgctacccgc cagccagaat ggctttgctc ctgtggttcg tgggattgca
      901 aagtccaacg ccaccgtcat cattcggcag aatggctacg tgatttatca gtcagccgtt
      961 ccacaaggtg cttttgagat caccgatctc aacaccgcaa gtacaggtgg cgatttggac
     1021 gtaaccatca aagaagaaga cggtagcgaa caacgattca cccaacctta tgcttcattg
     1081 gcgattctta aacgtgaagg tctgacagat gttgatgtca gcgtgggtga attgcgcgat
     1141 gaagacggat ttacaccgga cgtccttcag gcgcaaatac ttcatggttt ttcccacggg
     1201 atcactttat atggaggtat gcaggctgct gaaaattatg gttctgcagc tctgggtgtc
     1261 ggtaaagatc ttggcgcttt gggcgcaatt tctttcgatg tgacacatgc tcgtgcgaat
     1321 tttagccatg atgatacaga aacgggtcag tcatatcgct ttctctattc aaaactattt
     1381 gacgacacag acactagctt gcgcctggtt ggctatcgtt actccaccga gggctactat
     1441 accctcaatg agtgggcatc gcggcgcaac agccctgaag acttttggga aacaggtaac
     1501 cgacgtagtc gcgtggaggg aacgctaacg cagtcgttgg ggagagatta tggcaattta
     1561 tacctgacat taagccggca acaatactgg cataccgatg atgtcgaacg attaatgcaa
     1621 tttggctaca gcagtagctg gaagcgtctc tcgtggaacg tctcctggag ttattccaat
     1681 actgccagac aggggacggg gaacaaccat gccagtgata acaccagtga gcagatctac
     1741 atgctctctt tatctgttcc tttatcgggc tggtggggta atagttacgc cacctattct
     1801 gtttcgcaaa acgataattc cggtagctca catcaactcg gactcagcgg tacggcgctg
     1861 gaaagaaata acctttcatg gaatttaatg cagtcctata acagtcatga tgatgaggtt
     1921 ggcggtaata tgtccctgac ctatgatggc tcttatggca cggtgaacgg cagctataac
     1981 tacagccaaa attcccagag gctgaattat ggtatcagag ggggaattct ggcacacagc
     2041 gaaggggtaa cgttaagtca ggagttaggt gaaactattg ctcttgttaa agcacctggg
     2101 gccgccaggt tagaaataga taatatgcgc ggtgctgcga cggactggcg cggctatacg
     2161 gtcaagacac agctaaaccc ttatgatgaa aatcgggtag caatcagcga taactatttc
     2221 tcgaagtcga atatagaact tgataatacc gtcgttacga tggttcccac gcgtggtgca
     2281 gtggttaaag cggagtttgt gactcatgtg ggttatcgcg ttctcttcag agtgttaaat
     2341 gcaaatggta aaccggtacc ttttggagcc attgctgcga tacaagatgc aagtttggca
     2401 gattcaggaa ttgtcggtga ccgtggcgaa ctttatcttt ctggtctacc agaaaaagga
     2461 caggttacgt tatcctgggg agaaaacgcc tcaacaaaat gcatcttcaa ttattcattt
     2521 tcgacaccag aaagtgagag cggattaatt gaacagggtg tgacatgtca ttaaaaggat
     2581 ttttatgaac aagtatatca aacagtggtg ctttgctgtg tttatgctct cgttaagtag
     2641 tgtagcactt gcagctccta aaggtatctg tacccctgat aatggcgtat ttcacagtac
     2701 gcttgatttc tcgg
//
LOCUS       DAZPCS010000281         2660 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZPCS010000281 DAZPCS010000000
VERSION     DAZPCS010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.287"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..389
                     /locus_tag="ACHUQ7_004022"
     CDS             <1..389
                     /locus_tag="ACHUQ7_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR1137187.1"
                     /translation="HGVHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITEN
                     GSMITLSGIQYFHEMGIDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGW
                     LTRHLGYREVTITEKGYAAFKTHFHI"
     gene            complement(367..990)
                     /gene="tetR(B)"
                     /locus_tag="ACHUQ7_004023"
     CDS             complement(367..990)
                     /gene="tetR(B)"
                     /locus_tag="ACHUQ7_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HFR1137188.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            1072..2277
                     /gene="tet(B)"
                     /locus_tag="ACHUQ7_004024"
     CDS             1072..2277
                     /gene="tet(B)"
                     /locus_tag="ACHUQ7_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HFR1137189.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(2390..>2660)
                     /locus_tag="ACHUQ7_004025"
     CDS             complement(2390..>2660)
                     /locus_tag="ACHUQ7_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HFR1137190.1"
                     /translation="PIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDD
                     DVLKNLLNGWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
BASE COUNT          683 a          517 c          566 g          894 t
ORIGIN      
        1 accatggcgt acatgccaaa gtttccacgc cagtgcattt acgaaaagca cgtacttgct
       61 atgatcattt agctggcgaa gttgccgtta agatctatga ttccctttgt caacagcaat
      121 ggatcactga aaatggttca atgatcacat taagtggtat tcaatatttt catgaaatgg
      181 gaattgacgt tccttccaaa cattcacgta aaatctgttg tgcgtgttta gattggagtg
      241 aacgccgttt ccatttaggt gggtacgttg gagccgcatt attttcgctt tatgaatcta
      301 aagggtggtt aactcgacat cttggttacc gtgaagttac catcacggaa aaaggttatg
      361 ctgcttttaa gacccacttt cacatttaag ttgtttttct aatccgcata tgatcaattc
      421 aaggccgaat aagaaggctg gctctgcacc ttggtgatca aataattcga tagcttgtcg
      481 taataatggc ggcatactat cagtagtagg tgtttccctt tcttctttag cgacttgatg
      541 ctcttgatct tccaatacgc aacctaaagt aaaatgcccc acagcgctga gtgcatataa
      601 tgcattctct agtgaaaaac cttgttggca taaaaaggct aattgatttt cgagagtttc
      661 atactgtttt tctgtaggcc gtgtacctaa atgtactttt gctccatcgc gatgacttag
      721 taaagcacat ctaaaacttt tagcgttatt acgtaaaaaa tcttgccagc tttccccttc
      781 taaagggcaa aagtgagtat ggtgcctatc taacatctca atggctaagg cgtcgagcaa
      841 agcccgctta ttttttacat gccaatacaa tgtaggctgc tctacaccta gcttctgggc
      901 gagtttacgg gttgttaaac cttcgattcc gacctcatta agcagctcta atgcgctgtt
      961 aatcacttta cttttatcta atctagacat cattaattcc taatttttgt tgacactcta
     1021 tcattgatag agttatttta ccactcccta tcagtgatag agaaaagtga aatgaatagt
     1081 tcgacaaaga tcgcattggt aattacgtta ctcgatgcca tggggattgg ccttatcatg
     1141 ccagtcttgc caacgttatt acgtgaattt attgcttcgg aagatatcgc taaccacttt
     1201 ggcgtattgc ttgcacttta tgcgttaatg caggttatct ttgctccttg gcttggaaaa
     1261 atgtctgacc gatttggtcg gcgcccagtg ctgttgttgt cattaatagg cgcatcgctg
     1321 gattacttat tgctggcttt ttcaagtgcg ctttggatgc tgtatttagg ccgtttgctt
     1381 tcagggatca caggagctac tggggctgtc gcggcatcgg tcattgccga taccacctca
     1441 gcttctcaac gcgtgaagtg gttcggttgg ttaggggcaa gttttgggct tggtttaata
     1501 gcggggccta ttattggtgg ttttgcagga gagatttcac cgcatagtcc cttttttatc
     1561 gctgcgttgc taaatattgt cgctttcctt gtggttatgt tttggttccg tgaaaccaaa
     1621 aatacacgtg ataatacaga taccgaagta ggggttgaga cgcaatcgaa ttcggtatac
     1681 atcactttat ttaaaacgat gcccattttg ttgattattt atttttcagc gcaattgata
     1741 ggccaaattc ccgcaacggt gtgggtgcta tttaccgaaa atcgttttgg atggaatagc
     1801 atgatggttg gcttttcatt agcgggtctt ggtcttttac actcagtatt ccaagccttt
     1861 gtggcaggaa gaatagccac taaatggggc gaaaaaacgg cagtactgct cggatttatt
     1921 gcagatagta gtgcatttgc ctttttagcg tttatatctg aaggttggtt agttttccct
     1981 gttttaattt tattggctgg tggtgggatc gctttacctg cattacaggg agtgatgtct
     2041 atccaaacaa agagtcatca gcaaggtgct ttacagggat tattggtgag ccttaccaat
     2101 gcaaccggtg ttattggccc attactgttt gctgttattt ataatcattc actaccaatt
     2161 tgggatggct ggatttggat tattggttta gcgttttact gtattattat cctgctatcg
     2221 atgaccttca tgttaacccc tcaagctcag gggagtaaac aggagacaag tgcttagtta
     2281 tttcgtcacc aaatgatgtt attccgcgaa atataatgac cctcttgata acccaagagg
     2341 gcatttttta cgataaagaa gatttagctt caaataaaac ctatctattt tatttatctt
     2401 tcaagctcaa taaaaagccg cggtaaatag caataaattg gcctttttta tcggcaagct
     2461 cttttaggtt tttcgcatgt attgcgatat gcataaacca gccattgagt aagtttttaa
     2521 gcacatcatc atcataagct ttaagttggt tctcttggat caatttgctg acaatggcgt
     2581 ttaccttacc agtaatgtat tcaaggctaa ttttttcaag ttcattccaa ccaatgatag
     2641 gcatcacttc ttggataggg
//
LOCUS       DAZPCS010000282         2641 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZPCS010000282 DAZPCS010000000
VERSION     DAZPCS010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.288"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            94..1209
                     /gene="adrA"
                     /locus_tag="ACHUQ7_004026"
                     /gene_synonym="dgcC"
     CDS             94..1209
                     /gene="adrA"
                     /locus_tag="ACHUQ7_004026"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFR1137191.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMILCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLMFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(1226..2035)
                     /gene="proC"
                     /locus_tag="ACHUQ7_004027"
     CDS             complement(1226..2035)
                     /gene="proC"
                     /locus_tag="ACHUQ7_004027"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFR1137192.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            2155..2613
                     /locus_tag="ACHUQ7_004028"
     CDS             2155..2613
                     /locus_tag="ACHUQ7_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFR1137193.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNSRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
BASE COUNT          585 a          668 c          740 g          648 t
ORIGIN      
        1 agtgctaacg ggtgagctac gaaagtggct cacccgaaat ttcatacttc tgcctttagc
       61 cccgtctcta taatttggga aaattgtttc tgaatgttcc caaaaataat gaatgatgaa
      121 aactttttca aaaaagcggc ggcgcatggg gaggaacctc ctttaactcc tcaaaacgaa
      181 catcagcggt ccgggctgcg cttcgcccgt cgcgtcagac taccccgtgc ggttggcctg
      241 gctggcatgt tcttaccgat tgcttcaacg ctggtttcgc acccgccgcc gggctggtgg
      301 tggctggtgt tggtcggctg ggcgttcgtc tggccgcatt tagcctggca gatagcgagc
      361 agggccgtcg atccgcttag ccgggaaatt tacaacttaa aaaccgatgc agtattagcg
      421 ggaatgtggg taggcgtaat gggcgtaaac gtgctgcctt ccaccgctat gttgatgatt
      481 ctgtgtctga atttgatggg ggcaggcggc ccccgtctgt ttgtcgcggg tctggtgttg
      541 atggtggttt cctgccttgt caccctcgag cttacgggca ttaccgtgtc gttcaatagt
      601 gctccgctgg aatggtggct ctcccttccc attattgtcg tttatcctct gatgtttggc
      661 tgggtcagct accagacggc aaccaaactg gcggaacata aacgcaggtt gcaggtcatg
      721 agtactcgcg acggcatgac gggcgtgtat aaccgacgtc attgggaaac tatgttacgc
      781 aatgaatttg ataactgtcg gcggcataat cgcgatgcaa cgttactgat tatcgatatc
      841 gaccatttca agagcatcaa cgatacctgg ggtcatgatg tgggcgatga agcgattgtg
      901 gcgcttaccc gacagttaca aattaccctg cgcggtagcg atgtgattgg tcggtttggc
      961 ggcgatgagt ttgcagtaat catgtccggt acgccagctg agagcgccat taccgccatg
     1021 ttacgggtgc gtgaagggct aaacacgtta cgcttgccga atacgccaca ggtaacttta
     1081 cggattagtg tgggggttgc gccgctgaac ccacaaatga gtcactatcg tgagtggttg
     1141 aaatcggcag atttggcgct ttacaaagca aagaaagccg gacgtaaccg caccgaagtg
     1201 gcggcctgac gtccggcgaa agtcatcagg atttgctgag tttttctgat ttttccatac
     1261 acttcgtcat cgcttcgatc actgcggcac ggaagccttt ctcttccagt acgcgtaccg
     1321 cttcaatggt ggtgcctccc ggtgagcaga ccatatcttt cagtgccccc ggatgttctc
     1381 ccgtttccag caccattttt gcggaaccca ttaccgcctg agcggcaaat ttatacgcct
     1441 gggcgcgtgg catcccgccc agcacggcgg cgtcggccat cgcttcgata aacataaata
     1501 cgtaggctgg cgaagaaccg ctcacaccga ccaccgggtg gatcatcggc tcagcaatta
     1561 cttccgcttc gccaaagcag cggaaaatat tcagcacatc agcggtatct tctggggtta
     1621 ccagcgcgtt tggcgttacg gaggtcatcc cggcattaac cagcgcggga gtgttcggca
     1681 tggcgcggat aattttccgg tcatggccca gcgcgcgggc aagctggtcg agcgtgacac
     1741 ctgcagcaat agaaacgacc agagagtctt tattcaggct ggaggtgatt tcgctaagca
     1801 ctttaatcat gatgccaggt ttaacggcag caaaaatgat gtcggcgatt tgcgccactt
     1861 cttgcgccga ttctgcggcg ttgatgccga actggtcatg cagggcggcg actttatccg
     1921 gggagggggt gtatacccag atttgccctg gaagcacctg accgctggca atcagaccgc
     1981 cgagaatggc ttttcccata ttgccgcagc caataaaacc gattttcttt tccattgcct
     2041 cactcctgcc gtgaaattca ttgttttgat aatcgctggc agaagcataa acagaactat
     2101 gccggaaggc aaaagcgcga cacaatagag gattacctaa caaaggatga ctttatgaca
     2161 atttgggtgg atgccgacgc gtgtcccaat gtaattaaag agattttgta tcgcgcggcg
     2221 gaacgtatgc agatgccgct ggtactggta gcaaaccaga gtttacgcgt gccgccatcg
     2281 cgatttattc gtacgctgcg cgtcgcggca ggtttcgatg ttgccgataa cgaaattgtc
     2341 cggcagtgtg aagcgggcga tttggtgatc accgcagata tacctttggc tgctgaagcc
     2401 atcgagaaag gcgcagcggc gcttaattcg cgcggcgaac gttacacgcc agcgaccatt
     2461 cgtgagcgcc tgacgatgcg cgattttatg gataccttac gtgccagtgg gatccagacc
     2521 ggcggaccag atagcctttc acaacgtgac cgccaggcct ttgccgcgga gctggagaag
     2581 tggtggctgg aagtgcaacg tagtcgtggc taaatgtaat ttattattta cacttcattc
     2641 t
//
LOCUS       DAZPCS010000283         2611 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZPCS010000283 DAZPCS010000000
VERSION     DAZPCS010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.289"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            138..227
                     /locus_tag="ACHUQ7_004029"
                     /pseudo
     CDS             138..227
                     /locus_tag="ACHUQ7_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308311.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
     gene            529..1524
                     /gene="pgl"
                     /locus_tag="ACHUQ7_004030"
     CDS             529..1524
                     /gene="pgl"
                     /locus_tag="ACHUQ7_004030"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFR1137194.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(1565..2518)
                     /locus_tag="ACHUQ7_004031"
                     /pseudo
     CDS             complement(1565..2518)
                     /locus_tag="ACHUQ7_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
BASE COUNT          661 a          586 c          636 g          728 t
ORIGIN      
        1 tatctcaatg tactgttttt ttctttaaaa aaacagtaca ttctatttta taatgactgt
       61 gtattcccat ggaaggagat cacccaattc tcccccccga cttttgacgc agtgtcttgt
      121 taacaggggg attgaccatg cttattggct atgtacgcgt atcaacaaat gaccagaata
      181 ccgatctaca acgtaatgcg tttatttgtg ccggaagtga gttgattgtt gcgcgaatgg
      241 gaagttggcc agaagttgtt atggcatagt gtttacggtc gttgatacag gtaaacaaaa
      301 gcggggagag tcatactccc tgaatagtcg cgaaaatatc tgtggcattg tcaaattcgt
      361 gtcggtgact aatattttag taatatgcca ttcgaaatgg agtgctaact tattgattta
      421 attgatgtgc gatattgtat tttatcgtgt tttgttgtct gttttttatc cagtaagtgt
      481 tgttatatgt atgaaagata agggatttat catttttgag gtgtgttcat gaagcaaaca
      541 gtttatatcg ccagccctga gagccagcaa attcacgtct ggaacctgaa tcatgaaggc
      601 gcactgacgc tgacacaggt tgtcgatgtg ccggggcagg tgcagccgat ggtggtcagc
      661 ccggacaaac gttatctcta tgttggtgtt cgccctgagt ttcgcgtcct ggcgtatcgt
      721 attgccccgg acgatggcgc actgaccttt gccgcagagt ctgtgctgcc gggtagtccg
      781 acgcatattt ccaccgatca ccaggggcag tttgtctttg taggttctta caatgcgggt
      841 aacgtgagcg taacgcgtct ggaagatggc ctgccagtgg gcgtcgtcga tgtagtcgag
      901 gggctggacg gttgccattc cgccaatatc tcaccggaca accgtacgct gtgggttccg
      961 gcattaaagc aggatcgcat ttgcctgttt acggtcagcg atgatggtca tctggtggcg
     1021 caggaccctg cggaagtgac taccgttgaa ggggccggcc cgcgtcatat ggtattccat
     1081 ccaaacgaac aatatgcgta ttgcgtcaat gagttaaaca gctcagtgga tgtctgggaa
     1141 ctgaaagatc cgcacggcaa tatcgaatgt gtccagacgc tggatatgat gccggaaaac
     1201 ttctccgaca cccgttgggc ggctgatatt catatcaccc cggatggtcg ccatttatac
     1261 gcctgcgacc gtaccgccag tctgattacc gttttcagcg tttcggaaga tggcagcgtg
     1321 ttgagtaaag aaggcttcca gccaacggaa acccagccgc gcggcttcaa tgttgattac
     1381 agcggcaagt atctgattgc cgctgggcaa aaatctcacc acatctcggt atacgaaatt
     1441 gttggcgagc aggggctact gcatgaaaaa ggccgctatg cggtcgggca gggaccaatg
     1501 tgggtggtgg ttaacgcaca ctaaccgctg atttaaccgg cgcagtctct cctgcgccgg
     1561 tgtattaact tatctcctgt aacgcatgtc tctggcgttc gacgatattg gtccacaaat
     1621 tgtctttatc gtcagtccac aaattaatca gcaaggaaaa aaagcgttca gctgccggag
     1681 aaagcacggc atctttgcgg cgaataatcc ccaatgtccg acgtataacg ggttccacca
     1741 gtgggatacc aataatcgaa gaatagggcg catggggcat tgccagacca ggaagcgccg
     1801 aaatccccag tcctgcctcc actagtccta atgacgtcga aagatgacgc acttcgtaaa
     1861 accagtccag cttccagggc ttgtcggcta gctgttgttc tatcaacaat cggttgccgc
     1921 tggaggaacg tacgccaatc attttgtaac ccactaactc ctgccattct acaagctact
     1981 ttttggccaa tggatggtca cgccgacagg ccagcacgaa tggctcgttg accagtgggg
     2041 taaaatcaat ggatgaattt gtgacgttgt tcatgtttat gccaaagtct gattcgttac
     2101 acagcaccga ttccatgcaa ttattcgtgc cttgttccag aatccgcact ttaatattgg
     2161 gatacagctc attaaattta ccgattgcca gcggtaaaaa ataaaatact gcggtcggaa
     2221 tacacgctaa tgtcaccata ccacgatgat aagcattcat atcacgaata ttaaaaagcg
     2281 tttcatcgaa tttctttatt aattcccgcg cctccgggag caaccttttt cctgcttttg
     2341 ttaacgtaac cttgcgagtt gtacgctcaa atagttgaat atgtaaatct tcctccatct
     2401 tttttattct tcgcgttaat gcaggttgcg taatattgag taatttagcg gcattgttaa
     2461 atgaactgga ctctgccagt atgacaaatg ccttcatact tgataattca tgcttcatta
     2521 ttactccgga aaatggaagc gacgattttg gggggctggt cgttaaaaat tttaactgca
     2581 tttagccaac ttaaattaat gaaaaaatgt t
//
LOCUS       DAZPCS010000284         2544 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZPCS010000284 DAZPCS010000000
VERSION     DAZPCS010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.290"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..197
                     /locus_tag="ACHUQ7_004032"
     CDS             <1..197
                     /locus_tag="ACHUQ7_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFR1137195.1"
                     /translation="LVEGGIDLKDVNVGNMHFSEGKKQISSKVYVDDQDLADLRFIKQ
                     RGVNVFIQDVPGDQKEQIPD"
     gene            236..1039
                     /gene="agaC"
                     /locus_tag="ACHUQ7_004033"
     CDS             236..1039
                     /gene="agaC"
                     /locus_tag="ACHUQ7_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HFR1137196.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIRVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            1029..1820
                     /gene="agaD"
                     /locus_tag="ACHUQ7_004034"
     CDS             1029..1820
                     /gene="agaD"
                     /locus_tag="ACHUQ7_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HFR1137197.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            1821..>2544
                     /locus_tag="ACHUQ7_004035"
     CDS             1821..>2544
                     /locus_tag="ACHUQ7_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HFR1137198.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWL"
BASE COUNT          605 a          605 c          658 g          676 t
ORIGIN      
        1 aattggtaga aggtggcatt gacctgaaag atgtcaacgt cggcaatatg catttctcgg
       61 aagggaaaaa gcaaatcagc agtaaagttt atgtcgatga ccaggatctc gcggacttac
      121 gttttattaa acaacgtggc gtgaatgttt tcattcagga cgtccctggc gatcaaaaag
      181 aacaaatccc tgactaaatc taaaatcgcc ttaatattgg tttgaggtaa taaaaatgca
      241 tgaaataacc ctacttcagg gattatccct agcggcgtta gttttcgttc tggggattga
      301 tttttggctg gaagccttat ttttattccg cccgataatc gtttgtaccc taactggcgc
      361 tattctcggt gatattcaga ctggcttaat taccggtggt ctgacagagt tggctttcgc
      421 cggattaacc cctgcaggtg gtgttcagcc gcccaacccg attatggcgg gtctgatgac
      481 caccgtcatt gcatggtcta cgggcgttga tgccaaaaca gcaattggtc ttggcctgcc
      541 gtttagtttg ttaatgcagt acgtcattct gttcttctat tccgctttct cattatttat
      601 gaccaaagcc gataaatgcg cgaaagaggc ggatacggca gcgttttccc ggcttaactg
      661 gacaacgatg ctcatcgtcg cttcagcgta tgcggtgatt gctttcctct gtacttacct
      721 ggcacaaggg gcgatgcagg cgctggtgaa agcgatgccc gcctggttga cccacggctt
      781 tgaagtggct ggcggtattc tgcctgccgt tggttttggc ttgctgctgc gcgtaatgtt
      841 caaagcgcaa tatatccctt acctgatcgc cggtttcctg tttgtttgct acatccgggt
      901 cagcaacctg ttgccggttg ccgtactggg cgcaggcttt gcggtgtatg agtttttcaa
      961 tgcgaaatcc cggcagcaag cgcaaccgca gcccgttgcc agtaaaaatg aagaagagga
     1021 ctacagcaat gggatctgaa atcagtaaaa aagatatcac ccgtctgggc tttcgttcgt
     1081 cgctgctgca agcgagcttt aactacgaaa ggatgcaggc gggcggtttt acctgggcga
     1141 tgttgccgat cctgaaaaag atttataagg acgacaaacc gggcttaagc gcggcgatga
     1201 aagataacct cgaatttatt aatacccacc cgaatctggt cggattcctg atggggttat
     1261 taatttcgat ggaagaaaaa ggagaaaacc gcgacaccat taaaggcctc aaagtggcac
     1321 tgtttggccc aatcgccggg attggcgatg cgattttctg gtttactttg ttgccgatta
     1381 tggcgggaat ttgctcatca tttgccagcc agggaaacct gctggggccg attctatttt
     1441 tcgccgttta cctgcttatc tttttcctgc gcgtcggctg gacccacgtc ggttattcag
     1501 tcggcgtgaa ggcgatcgat aaagtgcgag agaactcgca gatgattgct cgctcggcaa
     1561 ccatcctcgg gatcacggta atcggcgggc tgatcgcttc gtatgtgcat attaacgtgg
     1621 tgacatcgtt tgccatcgac agtacccaca gcgtcgcgct gcaacaggat ttcttcgata
     1681 aagtcttccc gaacatttta ccgatggcct acaccctgct gatgtattac ttcctgcggg
     1741 tgaaaaaagc gcatccggtg ctgttaatcg gcgtgacttt tgtgctctct attgtttgtt
     1801 ccgcattcgg cattttgtaa atggaacgag gcactgcttc tggcggtgcc tctttactta
     1861 aggaatttca tcctgtgcaa acccttcagc aagttgaaaa ctatacggcg ttaagtgaac
     1921 gagccagcga atatttattg gctgtgatcc gtagcaaacc ggatgccgtg atttgcctgg
     1981 cgaccggagc cacgccatta ctgacgtatc attatctggt agaaaaaatc caccagcagc
     2041 aggttgatat cagccagctc accttcgtga agctcgacga atgggtggat ctgccattaa
     2101 cgatgcctgg cacctgcgaa actttcctac aacagcatat cgtgcagccg ctggggctac
     2161 gtgaagacca gctcatcagc tttcgctccg aagagataaa tgagacagag tgcgaacggg
     2221 taacgaacct gattgcgcgc aaaggcggtc tggatttatg cgttctcgga ttggggaaaa
     2281 acggtcatct tgggctgaac gaaccgggag aaagcctaca accggcctgc catatcagtc
     2341 aacttgatgc cagaacacag caacatgaga tgttaaaaac cacgggtcgc cccgtgactc
     2401 gtgggatcac cttaggcctg aaggatattc tcaatgcccg cgaggttttg ttattggtga
     2461 ctggcgaagg aaagcaggat gcgacagaac gttttctcac ggctaaagtc tccaccgcta
     2521 tcccggcttc atttttatgg ctgc
//
LOCUS       DAZPCS010000285         2491 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZPCS010000285 DAZPCS010000000
VERSION     DAZPCS010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.291"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..>2491
                     /locus_tag="ACHUQ7_004036"
     CDS             <1..>2491
                     /locus_tag="ACHUQ7_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFR1137199.1"
                     /translation="QEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMGLRFGRDV
                     NAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVITEEWRAH
                     QDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQPNNMTFIV
                     VGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRVNGIALYY
                     RLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVKIAPDYQS
                     LFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAVDQQAERD
                     LRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQDAFWEQM
                     VNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEISSKETLAE
                     NLTSLTLSNGAKVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANKAVSGSGV
                     GELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRITHSTINDN
                     IWASLQNAQIQALKTLDQRPAEKFAQLMYETRYADDRTKLLQENQIAQFTAADALAAD
                     RQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRATDNASVTV
                     KEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASGAYSVSSR
                     LSVDPQAKDISHLLAFTCQPERHDEQLTLANEVMVKRLTKGISEQELNEYQQNVQRSL
                     DIQQRSVQQLANTIINSLIQYDDPAAWTEQEQ"
BASE COUNT          730 a          578 c          602 g          581 t
ORIGIN      
        1 caggaagaag acaatgagcg cggcgtggct cattttgtag aacatatgat gtttaacggc
       61 acaaaaacat ggccgggtaa taaagtcatc gaaacatttg agtcaatggg cctgcgtttt
      121 ggtcgcgatg ttaatgccta taccagctat gacgaaacgg tgtatcaggt gagtttgccg
      181 actacgcaga aacaaaatct gcaacaagtg atggcaatct tcagtgaatg gagtaatgcc
      241 gcaacctttg aaaaactcga agtagacgct gaacgtggcg taattactga ggaatggcgt
      301 gcccatcagg atgcgaaatg gcgcacctct caggcgcgcc gccctttcct gctggctaat
      361 acccgtaatt tagaccgtga acctatcggc ctgatggata ctgtcgccac ggtcacgccg
      421 gcacaattgc gccaatttta tcaacgctgg tatcaaccaa ataatatgac ctttatcgtg
      481 gtcggcgata tcgacagtaa agaagcgctg gcgctgataa aggataattt aagtaagctt
      541 ccggctaaca aagcagctga aaatcgcgtc tggccgacaa aagccgaaaa ccacctgcgc
      601 tttaatatca ttaatgataa agaaaaccgg gtgaacggca tcgcactcta ttatcgcctg
      661 ccaatggtac aagtgaacga tgagcaaagc tttgtcgaac aagctgaatg gagcatgtta
      721 gttcagctgt tcaatcaacg tctgcaggaa cgcatacagt cgggcgagtt gaagactatt
      781 tctggcggca ctgcgcgcag cgttaaaatt gcacccgatt atcagtcgct gtttttccgt
      841 gtaaatgcac gagacgataa tatgcaggat gctgcgaatg cattaatggc agagttggca
      901 accattgatc agcatggttt ttctgctgaa gaactcgatg atgtcaaatc tacccgcctc
      961 acctggctga aaaatgcggt tgatcagcaa gctgaacgtg atttacgtat gctgaccagt
     1021 cgcctggcat ccagctcatt aaataatacg ccgttcttgt cgccggaaga gacatatcaa
     1081 ctttcgaaac gtctgtggca gcaaattacc gtgcaaagtc tggcggaaaa atggcagcag
     1141 ttaagaaaga accaggacgc attttgggag caaatggtaa acaatgagct tgccgccaaa
     1201 aaagcattgt ctcctgcggc tatcctggcg ctggaaaaag agtacgccaa caaaaagctg
     1261 gcggcttaca tcttcccagg cagaaattta tcgttaacag tagacgctga cccacaggcg
     1321 gaaattagca gcaaagaaac gctggctgag aatctgacat cattaacact ttccaatggt
     1381 gccaaggtta ttctggcaaa atccgcgggt gaagagcaaa agctacaaat tattgccgta
     1441 tcgaataaag gcgatttaag tttccctgcg cagcaaaaat cacttatcgc gctggcaaat
     1501 aaagcagtta gcggaagcgg cgttggcgaa ctctcctctt ccagcctgaa acgctggagt
     1561 gcggaaaact cggtaaccat gagcagtaaa gtcagtggca tgaatacgtt gctctccgtt
     1621 agcgcgcgga ctaataaccc tgaacctggt ttccagttga ttaaccagcg aatcacccac
     1681 agcacgatta acgacaatat ttgggcatcg ctacaaaatg ctcaaattca ggcgttgaaa
     1741 acgctcgacc agcgtccagc ggagaaattc gcccagctga tgtatgagac gcgctatgct
     1801 gatgaccgca cgaaattact gcaagaaaat cagattgcac agtttactgc cgcagatgcg
     1861 ctggctgccg atcgccagtt gttttcatct ccagcggata tcacgtttgt cattgtcggt
     1921 aatgtcgcag aagacaaact cgtggcgtta attacgcgtt acttaggctc aatcaaacac
     1981 tctgattcgc cattagccgc aggtaaacca ttaactcgcg cgacggacaa cgcatcggtt
     2041 actgtaaaag aacaaaatga acctgtggca caggtttcac agtggaagcg ttatgattcc
     2101 cggacacctg ttaatctggc gacgcgtatg gcgctcgatg cttttaacgt cgcactggca
     2161 aaagatctac gcgttaatat tcgtgaacag gcatctgggg catacagcgt ttcttctcgc
     2221 ctctcggttg atcctcaggc taaagatatc agtcatttgc tggcttttac ttgtcaacca
     2281 gaacgacatg atgaacagtt aacgttagcg aatgaagtga tggttaagcg tttgactaag
     2341 gggatcagtg agcaagaact gaacgaatac cagcaaaacg ttcagcgcag cctcgatatc
     2401 caacagcgta gcgttcaaca attagcgaac actattataa atagtcttat tcaatatgac
     2461 gatcctgcag catggactga gcaggagcaa t
//
LOCUS       DAZPCS010000286         2483 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZPCS010000286 DAZPCS010000000
VERSION     DAZPCS010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.292"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..823)
                     /locus_tag="ACHUQ7_004037"
     CDS             complement(<1..823)
                     /locus_tag="ACHUQ7_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR1137200.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLTSSPDYHGP"
     gene            complement(1422..1670)
                     /locus_tag="ACHUQ7_004038"
     CDS             complement(1422..1670)
                     /locus_tag="ACHUQ7_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFR1137201.1"
                     /translation="MRFEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(1933..2028)
                     /locus_tag="ACHUQ7_004039"
                     /pseudo
     CDS             complement(1933..2028)
                     /locus_tag="ACHUQ7_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2025..2372)
                     /locus_tag="ACHUQ7_004040"
                     /pseudo
     CDS             complement(2025..2372)
                     /locus_tag="ACHUQ7_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
     gene            complement(2399..>2483)
                     /locus_tag="ACHUQ7_004041"
     CDS             complement(2399..>2483)
                     /locus_tag="ACHUQ7_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1137202.1"
                     /translation="LIALARRRCDVLFAMMRDGTFYTPQGS"
BASE COUNT          638 a          468 c          605 g          772 t
ORIGIN      
        1 gcgggccgtg gtagtccggc gaagatgtta atctttgcag ggattgcaga gcatgggatg
       61 acaatgggtt atcactaata tctattgaac attcattcct cagattaaga atgctttccg
      121 ggatatgact tatctgattt atgtcaaaat aaccgttcct taaattttcc ggcaggtatg
      181 gaatatcggt taacatattg tcgattgccc atatggagtg aatgttgtga ggtagagggg
      241 ggagttcact aagaagattc cctattgcgc taatctcttg taaagaaaat ggtaaaggtg
      301 gtaaaactgc cagcccatta catgatacat ctaatgtttc cagtagttct ggtaaaacag
      361 gaagagaaca taattgatta tttgagacat gaagctcctt caaagatatg gggagtgtgg
      421 gtagtgtgat tagttgattg tgggacgcat tcaataattt aagtccttga ggcaaagcag
      481 gcagttcaat aagtctgtta tagctgacat taagttgtgt aagggacgca ggcaatgggg
      541 agattaagct taaattattt ttacttatat taattgattt aattcccggg gggatttcag
      601 gtaatgttgt caggcctaat tcagacaagt ccaggttcgt ctcttggttt tgtagacatg
      661 atactagtcg ctgaaaagcg atgtctcgtt gttcttcttg tatgcggtta tttttccatt
      721 cagtccaatg ggttagataa ttttcatatg cgcggctagt gtcgattgaa taagtggaga
      781 acgaatttga aattaatctg tgattgttat ttgtcgggag cataaatatc agggtgtatt
      841 ctttatgttg agaggtgttg gattcttttt tatctgcttt gcaatgttat tggttccctt
      901 cagcagtaga caggaacttc tggaggaggc ggatatatag tttgtgtaag catattaact
      961 ccatatgtta tatattgaag aacttctgct ttacactatc ctaaactgga tcggtttaat
     1021 gtaaaaaaac gatacttttt gggggggggc aatttttcct taaggtatga aaaaaggagc
     1081 tgaagctata taatagctat taatgccact ggtatcagtg aatcatgtat ataaaaaact
     1141 gtgttaagga ggctgtccat tagcggtttt ttattcttct ggaaccgcgg catctttgcg
     1201 tatctttttg tcttctcacc gtctggtcgg tgttttgctg agactgctaa tttcctgtct
     1261 ttgctagtgc tgtactggta ctgcccaatc atgattggcg ggggcgggcg gagttgagac
     1321 cgcagacacg tcgtatgcaa gaacgtgctg cggctggctg gtgaacttcc gatagtgcga
     1381 gtattgaatg atttccagcc gttactgatt ttacgtgcta attagtgaac aaaccactcg
     1441 tcagcagact cccaggtatc tttcagagtt tcctgaacaa aagttttagc tgaatcttta
     1501 tcggcggtgc gcgtaacaga aaggccatcg ttgctggtgg cttttacgat cacctctaca
     1561 tcgtcataac gtttactgat gcgtcgggtt aattcttcct ttaacgcatc cacagcaccg
     1621 gttggcattt tagtcatttt ctctttggct atgcagattt caaaacgcat aaaaatccct
     1681 ctgcactgtg tttttataca gtattatttt taactgtatg gataaacagt gtcaagagtg
     1741 agggaaagtg attttttggg gcatgcatgg ggcaaaaata tttgttgtgg ggcattttgg
     1801 ggcatgattg ggacactttg ttacatatga actttgccga ttttcatatg acatggcaat
     1861 ccaacgcttt gattattcta aagaaaacac atgctcttgg gcgttcttta gtgattttta
     1921 aaaatcccgt atcacgcagt taaagtggcg ggcatactct tcaaggctgg tgatccccaa
     1981 ccgcacccat ttaggatgtg accattgcgg tagaccgatg taaatcataa cgcgtgaagg
     2041 atctcttcca cgctacgcac acgggcgatg cgcgggtaga tatgattaat gctgttattg
     2101 tgttgctcgg cgctggcggc actacaggcg tcttcggcaa tcaccagatt aaagccaagt
     2161 tcccaggcat tgcgggcggt ggattcaaca ccgatattgg tcgagatccc acataacact
     2221 attgtatcga tacctcggcg gcgcagttgc aactccagat ccgtaccgta aaacgcaccc
     2281 cactgacgtt tgatgatttc gatatcactg tcagttgcac ctaatgcagc tggatgttgc
     2341 caccagtttt cgggcaacac tttagcgggg gagtgcccct attaagttgt caagcatgtt
     2401 atgacccctg cggggtataa aaagtcccgt cgcgcatcat ggcgaacaga acgtcgcagc
     2461 gtcgtctcgc cagggcgata agc
//
LOCUS       DAZPCS010000287         2463 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZPCS010000287 DAZPCS010000000
VERSION     DAZPCS010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.293"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(746..2350)
                     /locus_tag="ACHUQ7_004042"
     CDS             complement(746..2350)
                     /locus_tag="ACHUQ7_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1137203.1"
                     /translation="MIQLSSSHKLPAVYYLYQRNGVYYFRLRVRQSNNDRMTSISLRT
                     KDRRTAMAYSRHIKAALKAIHADRPNATYEEMREHLKDIAECELSMGRSDLFEPDMRD
                     IYRDQYGELGESLTDALASEPLSIDQHRYINEALKVLKACMRRIEAGDSQPLIDYVDL
                     FNDIDRQDNQADSVSLSVNAPEVKPEVTPSITIASLFEQYEAENYQNWKPATLRENKA
                     SHAALIEIFDHLGLNADANRADMLRVRDVLQQLPRNRKQRFKDVPLADLLSREDKTDC
                     LDVVTINNKYLIKMAAVFRWAVRNDLIKKNMTEGLELKVPQRKASGARNAFSTEQVGQ
                     LLVAAKAYSQKTSGKPYHYYVTALAAITGARLNEIAQLQVKDVRTTEAGTVYIHINED
                     DSSLPGKSIKNAHSDRCVPLVDGAYGFILADFMALVETRRGADGDDAMVFDGLRLMKN
                     GYGEQVSKWFNRTLLPKVLVDRSGLAFHSFRHTVAAQLKQHGVELAYAQAIMGHSSGS
                     ITYDRYAKEVEVDRLVNVMADVYKET"
BASE COUNT          648 a          575 c          526 g          714 t
ORIGIN      
        1 aagacctata ggtttggtga ttgattatta taaatatcat caccccttaa ggtattccct
       61 tagagttaat ctttaattgg tctttaatta aatcttttat ttaatactta aagttaatct
      121 ctaattgtgt ctttaagtta actttaagta atactataag ttaatcttta attgtgtctt
      181 taagttaact ttaagttaag cccctgtagg gactagagac cataattaag atgattgtgt
      241 cttattgcta ttcacttatc gactgaccca gagcacacag gaagcattca ctttaagtag
      301 aacgcttggt gtctgtaatt ggtcatcccg cccctgaaag gatgcgataa gttacggtta
      361 gttgtcctag aagagaaaac tttttgtgtc ccttctccct atagtgagtc gtattaatta
      421 tccttgacaa gtagagaaaa ccatgagtca aaaacatgac cacacaggtc tgtagtaagt
      481 gtcatcctag aagagaaaac tttttgtgtc ccttctccct atagtgagtc gtattaatta
      541 ccccttgaca agcagagaaa actatgagtc aaaaacatga ccgcacaggt ctgtagtaag
      601 tgtcatccta gaagagaaaa ctttttgtgt cccttctccc tatagtgagt cgtattaatt
      661 atcccttgac aagtagagaa aactatgagt caaaaacatg accacacagg tctgtagtaa
      721 gtgtcatcct agactatggt ctcctctagg tctccttata aacatcagcc atcacattga
      781 ccagcctatc aacctcgacc tctttagcat aacggtcgta cgtaatagac cctgaactat
      841 gccccatgat cgcctgtgca tacgccaact cgaccccatg ttgttttaac tgggccgcaa
      901 ctgtatgtct aaacgagtga aacgctaagc cgctacggtc tacgaggact ttaggcaaca
      961 acgtccgatt aaaccattta ctcacctgct caccgtaacc gttcttcatg agacgcagac
     1021 catcgaacac catagcgtca tcaccgtcag cgcctctacg ggtctctaca agcgccataa
     1081 agtcagccaa tataaaacca taggccccat cgaccagagg cacacagcgg tcactatgag
     1141 cgttcttgat gctcttaccg ggtagactac tatcgtcttc attgatatga atataaacgg
     1201 ttcccgcctc agtggtcctg acgtctttga cctgtagttg ggctatttca ttgagtctcg
     1261 ccccagtgat agcagccaag gcggtaacat aataatgata cggcttacca gaggtcttct
     1321 gagagtatgc cttagctgcg accagtagtt gccccacctg ctccgtagag aaggcgttac
     1381 gggcccctga ggctttacgc tgagggactt taagctccag accctcagtc atgttctttt
     1441 taatcaagtc attgcgcaca gcccacctaa agacagcagc catcttaata agatacttgt
     1501 tgttgatggt aacgacatcc agacaatctg tcttatcttc acggctcaac aggtcagcaa
     1561 gtgggacatc cttgaagcgc tgcttacggt tcctaggtaa ctgctggagc acatcacgga
     1621 ccctaagcat gtcagccctg ttagcgtctg cattgagacc cagatggtcg aatatttcga
     1681 ttaacgcagc gtgtgacgcc ttgttttccc ttaaggttgc aggcttccag ttctgatagt
     1741 tctccgcttc gtattgttca aagaggctcg ctatggtaat gctaggcgta acttctggtt
     1801 ttacttctgg agcattaaca gatagggaca cagagtctgc ttggttatct tgcctatcaa
     1861 tatcattaaa aagatcgacg taatctatca gaggctgact gtctcccgct tcgatacgcc
     1921 tcatacaggc ttttagcacc ttcagggcct cattgatgta acgatgctgg tcaatgctta
     1981 gcggctcact agccagagca tcggtaaggc tctcaccaag ttctccgtat tggtcccggt
     2041 agatatccct catgtcaggc tcaaagagat ctgaacggcc catagacagt tcacactcag
     2101 cgatgtcctt aaggtgttcc ctcatttcct cataggtagc gttgggtctg tctgcgtgta
     2161 ttgccttgag tgctgccttt atgtgccttg agtaagccat agcggtgcgt ctatccttgg
     2221 tccgtaatga tattgaggtc attctatcat tgtttgattg tctgacccta agacggaagt
     2281 agtagacgcc gttacgctgg tagagatagt agactgctgg aagtttgtga ctactggata
     2341 gctgaatcat atgtctgcat gccctgagag ggttacacag tgggttacag taagtaactc
     2401 tgcaaggagg cttaaagccg tatcccttag gattcagtag gttggagtac tttaagaaag
     2461 gtc
//
LOCUS       DAZPCS010000288         2450 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZPCS010000288 DAZPCS010000000
VERSION     DAZPCS010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.294"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(38..583)
                     /gene="ygeQ"
                     /locus_tag="ACHUQ7_004043"
                     /pseudo
     CDS             complement(38..583)
                     /gene="ygeQ"
                     /locus_tag="ACHUQ7_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338841.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgeQ"
     gene            complement(1245..2234)
                     /locus_tag="ACHUQ7_004044"
     CDS             complement(1245..2234)
                     /locus_tag="ACHUQ7_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1137204.1"
                     /translation="MNMTKNAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQDNIPFPVEVLPTRNSLPTPIIAHTLADRSVLVPVRGGKRPEVVT
                     APSGTEITVEPIEQAILVSHQTKLWDQKSTTGFTQGTLQQDALNICDNVIRTINSKMV
                     DVLESSKLLKTVELPALTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESHLKA
                     LSRLAAKQGFGGEDAIVDMLGTDVAYYNGEDRGVFMMAKRFTALSFGCFRHDGESITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLSFTKEAN"
     gene            complement(2318..>2450)
                     /locus_tag="ACHUQ7_004045"
     CDS             complement(2318..>2450)
                     /locus_tag="ACHUQ7_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1137205.1"
                     /translation="REQVKAERRTYGENLRSATHSAGRSRAAIVADLESGGNWMDSL"
BASE COUNT          653 a          560 c          589 g          648 t
ORIGIN      
        1 gcttaggcct gtgtccatat tacgtgggta ggatcagaat tccatccagt cgttttgggt
       61 tgctaatgat gtcctgcatc cgcttaatct ggtcgggcgt agacatataa ccccacaggg
      121 tgagcagaac cttttttgcg gctacactat ttccttggtc tgatactaat gccatatagc
      181 cccataaact atccctcagc cagacagcat catagtgggt ttctgccgct gttttgtcat
      241 cttcgaaagt agaagcaaga ataaacgttc cccagggttt ctccgtcaac gttaactttc
      301 gtgtgttttc tgttaatgtt agcaatcctt gagtttcgtc tttggtatag tgactcttta
      361 tttcattttt taatttggct gagtgaatca gaagacgata attaggatac ttatctggat
      421 ttaacgataa ataactttta tctgttgctg cccccctgga attaagaatg ttaaatacag
      481 attcgcttgt cacttctgcc tctgtcgcct ggcatgtgcc gcacgaaaat ataccagcga
      541 gcagaagagt taatgcagat ttccctttca taaaataatc catcaatcgt ttgttaaaag
      601 cgtagataac cacttattca acaacttgaa aataaggtat tttacctgag ttgttacaaa
      661 aggattgcat tgcgtaaacg ctttttattt acaaaaaaat ggggaagtgt tacagcgatt
      721 gcgaaaagcc tgaagtgatt gaattaagat aatatagaaa atcagataca gcaaaaatgg
      781 cagattgatc aggtgaagat aaattggagc gggcgaaggg agcctacgac aaatcgaaga
      841 caaccagcta acctattgtt tcttaaagac aggtgaatcc agttggttgt agtgatagtc
      901 acacatttag agatagaagg tcaagacttg aagcacgtaa tattccctct aaaggaatag
      961 aactcacggt ctgactaccc ggcccttttg gtctggctca ggtcaggcta tgggggcttt
     1021 tggctcgctc tttatattag atgaggtctc acatttttca gtcaaaactc atcgtctaat
     1081 agcccatagc cagaccacca gtcccagctt tagcaggaag accacgagat agactttgtg
     1141 taggtccatt tggccctcct actttctgta ttcacaaact agattactag tacaaaataa
     1201 aggccagaca tccagttcgg aagtctgacc aatagtcgtt attgttagtt agcttcttta
     1261 gtgaagctca ggcgtttgac tacaggcagg acagcagtag ccgagccagt acccatcttg
     1321 atggtcgtag cagcttcagc tacaacaaac accttgccga gaatctcaag gtcgtgagac
     1381 tgggagtctc catcacgtga aaggaccaca gtaatgctct cgccatcgtg acggaagcaa
     1441 ccgaagctca gagccgtaaa acgcttagcc atcatgaaca cgccacggtc ttcaccgttg
     1501 taataggcga cgtcagtccc cagcatatcg acgatagcgt cttcaccacc gaagccctgc
     1561 ttagcagcca gacgggagag agccttgagg tgtgactcat gggcgataat gccgtagtca
     1621 gaaacctcag aaccgaaaga agactccgtg ttctcgtaca gggcatccat gatagcgtca
     1681 gccttagcgg tcaggctacc agtcagggcc ggaagttcta cggtcttcag cagctttgag
     1741 gactccagca cgtcaaccat cttagagtta atggtacgaa tcacgttatc gcagatgttc
     1801 agtgcgtcct gctggagcgt cccctgagtg aaaccagtgg tcgacttctg gtcccaaagc
     1861 tttgtttggt gagacacgag gatcgcctgt tcaatcggct caacggtgat ttcagtgcca
     1921 gacggagcgg tcacaacttc aggtcgctta ccaccacgga cagggaccag cacagagcgg
     1981 tcagccagag tgtgagcaat gattggagtc ggtaagctgt tacgggtcgg caggacttct
     2041 acagggaacg ggatgttatc ttgagcgtct ttaagtacac cgttcagcat agccttaaca
     2101 gtcttagcta actggagggc atagacctga gggtcggcgt tagtcggcag accttcattg
     2161 acctgaatgt tgtcggtatc ggtgatgcgt actggctcaa tgccaaactg gaaagcgttt
     2221 ttagtcatat tcattataat taatctcctt gagtcaaata cttgaagtcc ctaacaggca
     2281 gtcagggacc gtgtgttctc agatgaggga cgcttgctta caagctgtcc atccagttac
     2341 cgcctgactc aaggtcagcg acgatagcgg cacgtgagcg accagcggaa tgagtagcgc
     2401 tgcggaggtt ttcaccatag gtccggcgtt cagctttgac ctgttcgcgg
//
LOCUS       DAZPCS010000289         2436 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZPCS010000289 DAZPCS010000000
VERSION     DAZPCS010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.295"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..360
                     /locus_tag="ACHUQ7_004046"
     CDS             <1..360
                     /locus_tag="ACHUQ7_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFR1137206.1"
                     /translation="PPQEETVVSIAVDPESPAQYLQRQKPQREEMPVYTRWVKTQKCM
                     TCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKDFEEKHGSQLLLL
                     IRFLMHARNSGVLKWKA"
     gene            361..726
                     /locus_tag="ACHUQ7_004047"
     CDS             361..726
                     /locus_tag="ACHUQ7_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR1137207.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVR
                     GQLVPGGRLGIKITELGCA"
     gene            723..1411
                     /locus_tag="ACHUQ7_004048"
                     /pseudo
     CDS             723..1411
                     /locus_tag="ACHUQ7_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310294.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
     gene            1605..1680
                     /locus_tag="ACHUQ7_004049"
     tRNA            1605..1680
                     /locus_tag="ACHUQ7_004049"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1638..1640,aa:Ile,seq:cat)
     gene            1682..1758
                     /locus_tag="ACHUQ7_004050"
     tRNA            1682..1758
                     /locus_tag="ACHUQ7_004050"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1716..1718,aa:Lys,seq:ttt)
     gene            1761..1836
                     /locus_tag="ACHUQ7_004051"
     tRNA            1761..1836
                     /locus_tag="ACHUQ7_004051"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1794..1796,aa:Thr,seq:tgt)
     gene            1840..1914
                     /locus_tag="ACHUQ7_004052"
     tRNA            1840..1914
                     /locus_tag="ACHUQ7_004052"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1873..1875,aa:Gly,seq:tcc)
     gene            2207..2422
                     /locus_tag="ACHUQ7_004053"
     CDS             2207..2422
                     /locus_tag="ACHUQ7_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFR1137208.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LVLGFLTYLTNLYFKIREDKRKAARGE"
BASE COUNT          640 a          520 c          653 g          623 t
ORIGIN      
        1 ccaccacagg aagagacggt ggtcagcatt gccgttgatc cggaatctcc ggctcagtat
       61 ctccagcgcc agaaaccaca acgggaagag atgcctgtat acacgcgctg ggtaaaaacg
      121 cagaaatgca tgacgtgtgg caatcaggca gatgatccgc accacatcat tggtcatgga
      181 ctgggaggga tgggaacaaa ggctgacgat ttgtttgtta ttccgctgtg tcgtaaatgc
      241 cataacgaac tacacgccgg ggtaaaagat tttgaagaaa aacacggtag tcagttgttg
      301 ttgctgattc gttttttaat gcacgcgaga aattcgggtg ttctgaagtg gaaagcataa
      361 atgaccgaac gcatagaatt tgttttgcct tacccgccaa cggtgaacac ctactggcga
      421 cgtcgtggca gcacatattt tgtatcaaaa gccggtgagc gttatcgccg tgatgtggcg
      481 ctaattgttc gccagcagcg actgaaatta aacctgtccg gaaggctggc gataaagatt
      541 attgcagagc caccggataa gcgccgtcgt gacctggaca atatcctgaa ggcaccgctg
      601 gatgcgctga cgcatgctgg actacttata gacgacgagc agtttgatga aatcaatatt
      661 gtgcgcggtc agctcgttcc tggtgggcgg ctggggataa aaatcacaga actggggtgc
      721 gcatgaataa ccagtattta cagtttgtgc gtgagcagct cattatcgcc accgctgatt
      781 tgagtggggc aacaaaaggt cagcttgaag cctggcaaga gaatgccatg ttcgatacag
      841 ggcgttacag gcgtaaaaaa atccggtacc gcgatgaagt gactggaaaa atgataacgc
      901 gggataatcc accaatcccg ggaaagcaat cgctggcgaa ggggacgtca attcctctgg
      961 tcagtccggt tgagttttcg acatcatcgt ggcggcgggc tgttctgtct cttgaagagc
     1021 actataaagc ctggttgttg tggtgttaca gcgggagtat ttgttgggaa tatcagatcg
     1081 cgataacaca gtgggcgtgg aatgaattta atgctcaatc cggtaccaga aaaattgcag
     1141 ggaaaacgca ggaacgcctg aaaaaattaa tctggctggc ggcgcaggca gtaaaagcag
     1201 aactttttgg tggggaaggt tatgaatacc aggagctggc attactggcg ggagtgacaa
     1261 ctaaaaactg gtccaaaaca tttactcgtc actgggttgc aatgaaacac atttttcacc
     1321 gactggatag tgaggcttta ttgtttgtaa tgagaacacg ttcaaaaaaa agacggcatt
     1381 ttcaaagcaa agtgttgcaa aagtagattg aaaggcatat attcatgcaa atctgatact
     1441 ttgccgattt tgtacgtgat ggcaaaagca aacaaaaccc gcccacaagc gggttttttg
     1501 tgccacttat ctcggataga catggtgaat gcgctggtgg aggagataag ggtgattttt
     1561 gaatgcttgc aacattgatt tcgtaacgtt attatcctgc gcccggccct ttagctcagt
     1621 ggtgagagcg agcgactcat aatcgccagg tcgctggttc aaatccagca agggccacca
     1681 accgccacta gctcatcagg aaagagcgtc aaccctttaa gttgagtgtg cgaggttcga
     1741 gtccccggtg gcggtccagt gccgacttgg ctcagtaggt agagcaactg acttgtaatc
     1801 agtaggtcac cagttcgatt ccggtagtcg gcaccatatg cgggcatcgt ataatggcta
     1861 ttacctcagc cttccaagct gatgatgcgg gttcgattcc cgctgcccgc tccagtcaga
     1921 gtctttcagt ctgcgatgat gggaaatccc ggagtgactg aaagacgttt aagttatgaa
     1981 tgatcgcttt tttttgcaaa attgctgtgc agaaatacta accttcgggc aggcgatcat
     2041 tcataagcac tctgctttta ttccgattaa ctgtgagtgg tttgttggat agagtgcttt
     2101 ccttattgta tatatcgttt cgcccgcttt tgcggttttt tcttttcaaa tccctttcat
     2161 ttctcagtgt aaaactacgc catccgttat ttgcggaggt gaggctatga aatccatgga
     2221 caaaatttca acgggcattg cctacggcac ctccgcaggc agtgctggct actggttttt
     2281 acagtggctt gatcaggtca gtccgtcaca gtgggctgcg attggtgtac tggggagtct
     2341 ggttctgggc ttcctgactt atctgacaaa tctgtacttc aaaatcagag aagacaagcg
     2401 taaggctgcg agaggtgaat aatgtcgcca tcatta
//
LOCUS       DAZPCS010000290         2398 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZPCS010000290 DAZPCS010000000
VERSION     DAZPCS010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.296"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            58..1005
                     /gene="icsP"
                     /locus_tag="ACHUQ7_004054"
     CDS             58..1005
                     /gene="icsP"
                     /locus_tag="ACHUQ7_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HFR1137209.1"
                     /translation="MKLKFFVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGSRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVLSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
     gene            complement(1567..1939)
                     /locus_tag="ACHUQ7_004055"
                     /pseudo
     CDS             complement(1567..1939)
                     /locus_tag="ACHUQ7_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            2183..2302
                     /locus_tag="ACHUQ7_004056"
                     /pseudo
     CDS             2183..2302
                     /locus_tag="ACHUQ7_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
BASE COUNT          687 a          495 c          528 g          688 t
ORIGIN      
        1 tttatcttat aaagtaagaa gatcatggac atttcaacca aaaaagtaga gttctcgatg
       61 aaattaaaat tctttgtact tgcactttgt gtacctgcga tctttactac acatgctacc
      121 actaactatc cacttttcat accggacaac atcagtacgg atattagtct aggatctctg
      181 agtggcaaaa caaaagaacg cgtttatcat cccaaggaag gggggagaaa aattagtcaa
      241 ctggactgga aatacagtaa tgcgactatt gttagaggtg gcatcgattg gaagctaatt
      301 ccaaaagtgt ctttcggagt ttccggttgg actactttag gtaaccagaa agcaagcatg
      361 gttgataaag actggaacaa ttccaatacc cctcaggtat ggaccgacca gagctggcat
      421 cccaatacgc atctccgtga tgctaacgaa ttcgagctga atcttaaagg ttggttatta
      481 aataatttgg attatcgact tggactaata gcaggttacc aagagagtcg ttacagtttt
      541 aatgcaatgg gagggagtta tatttatagt gaaaacgggg ggagcaggaa taaaaaaggg
      601 gcacatccta gtggtgaaag aacaataggt tacaaacagc tcttcaaaat accttatatt
      661 ggattaactg ctaattaccg ccatgagaat tttgagtttg gagcagaact gaaatatagt
      721 ggttgggttc tttcatctga tacagataaa cactatcaga ctgagacaat ttttaaagat
      781 gaaataaaaa accaaaatta ctgctctgtt gctgcgaata ttggatacta tgtcaccccc
      841 agtgcaaaat tttatataga aggctccaga aattacattt ctaataaaaa aggtgataca
      901 tctctttatg agcaaagtac caatatatct ggcaccatta aaaatagtgc aagtattgaa
      961 tatattggtt ttctcacttc cgcaggtata aagtatattt tttgaattgg ttcaccgagt
     1021 tgattacgtt ttcatggcta aaaattgccg cccattgtga gtcatattcg ccctgacttt
     1081 ccagaaccat acgaactgcc cgttgacgga cttcggggga aaaacgtgta tttttagtca
     1141 ttctgtttac ctctttctca gggcgtttag tctccaggat ttccggggcg gttcaaaaaa
     1201 ctcttcgttg attactgtgg acttactgtt ggcattaccg accctgagac cggagaaata
     1261 agaaccgctt agatcatcgt ggctgttctc ggggtatcaa gttacacatg ggcagaggct
     1321 acccaaggtc tactattatc gcctgaaagc gctgatggaa cagtgctatc agggacatgc
     1381 tgatgaaaat acctaaataa tcagccagca ctctatcttt ccaaatccac agcatagcaa
     1441 agagagcaaa agagcctgta aattcagaaa ttaaggaaac gcctcttggt gtacataatc
     1501 tatcttatgt taaatgaaat ctaatttctt gattatatgc tggatcgttc gtgggtggct
     1561 ttgcccctat atttccagac acctgttatc attggcccgc tgccgcagat attcccgtgg
     1621 cgagcgataa tccagcgcac tatgcggatg ccattcgtta taatgctcga atgcctctgc
     1681 aaggttcttt gctgccgtta acccgtcagg tttgggcatg atactgatgt agtcacactt
     1741 tattgttttc acgaagctct ttgctattct gttactctcc ggactccgca ccgccgtgtt
     1801 cttcggttca acaaagtccc aacatccggg tgaactggcg tgtttcatta gctcggtagc
     1861 atgaaccatt atccgtcagt cacttcactg gagacgccgg aagctcgttg ccgaagcggt
     1921 gttccaccgc tcccagcatg tcgccaacgg gagagcatct acggtctgat tgaactgttt
     1981 attcaaattg tccaccgtct gtcagtacgc tctgaacgtc ggctggttaa aacactgctc
     2041 gctgattttc agagggttca cggtaaaaca gcacttttat tccggattgc ggaagcggct
     2101 ctgaataatc cggatgggct ggttaaagag gttgtgtacc atctgcatat tgttgaacca
     2161 gatcgcagcg gtaagagaag ttcatcatac ttggcgcagt tacgcgatgt ttctgctaga
     2221 ggtgatgccg taaagaacgg cagaacatta ccggaacaag atagcggtct acctgcactg
     2281 gtatcagacc cggggcttcc ccggatgatc tccacggtac tttaggtact gtgtctatat
     2341 tatgtaggta ggatcaatta actttgtagt tgaaattctt tcattgaaat aatgaata
//
LOCUS       DAZPCS010000291         2396 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZPCS010000291 DAZPCS010000000
VERSION     DAZPCS010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.297"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(133..453)
                     /gene="psiF"
                     /locus_tag="ACHUQ7_004057"
     CDS             complement(133..453)
                     /gene="psiF"
                     /locus_tag="ACHUQ7_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFR1137210.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQALKGDDRNKFMSACL
                     KKAA"
     gene            complement(572..1987)
                     /gene="phoA"
                     /locus_tag="ACHUQ7_004058"
     CDS             complement(572..1987)
                     /gene="phoA"
                     /locus_tag="ACHUQ7_004058"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFR1137211.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTDVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTYTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(2088..2348)
                     /gene="iraP"
                     /locus_tag="ACHUQ7_004059"
     CDS             complement(2088..2348)
                     /gene="iraP"
                     /locus_tag="ACHUQ7_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFR1137212.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPCQ"
BASE COUNT          543 a          628 c          570 g          655 t
ORIGIN      
        1 ggaaaggctg ggccacatcc ctccggcaga agcagaaaaa gcttattatg cttccatcgg
       61 aaacgatgat ctggcagcct gagttcacag ataaaacact ctccaggaaa cccggggcgg
      121 ttcaatcagg tatcaggcgg ctttcttgag gcaggcactc ataaacttat tacgatcatc
      181 acctttcaga gcttgttgcg ttgcttgatt attacattca cgcatctttt gctgctgtgg
      241 cgtcaaactt ttttcaccag gcgcagactt gctgttcttc aggcaatcac tcatgtaggt
      301 cttacgagca tcccctttca acgcctgcgc cgtcgcctgc tgattacagg aggtcatacg
      361 ctgttgttgt ggggttaagg ttctctcggc agcgccgacg gtggttaaaa aaaccagacc
      421 aaaaagcaag gtaaccagta atgttatttt catagcacca tccctcttca tgttttaacc
      481 atgagcgttt gcgcccgtga tctgccatta agtctggttg ctaacagcaa aaaaaccacc
      541 cggcagcgaa aattcactgc cgggcgcagt tttatttcag ccccagagcg gctttcatgg
      601 tgtagaagag atcggtctgg tcggtcagtc caacaacatt ggcggcatgc gggccatacg
      661 ccgcaatacg caactgactg ccggtatgtt cttgtgaatc ctcttcggaa ttcccgtaac
      721 tcatcaccat cactgcgcca tctttagtat ttagcgcctg agtgaggccc ggcgctttgg
      781 tatccggcgc aacaacctgg ctggcgtggg cgtgatcagc ggtgactatg accagcgtgt
      841 taccctcctt tttagcgaat tccagcgccc gttgtacggc ttcatcgaga tcgaccgtct
      901 cgccaatttg cccacaaggg ttcgcagcgt gatcctgttt atcgattgac gcaccttcaa
      961 cttgcaggaa aaatcctttc tcatttttac tcaacaattc aatggctttg tcggtcatct
     1021 gtgccagggt tggtacactg tcattacgtt gcggattagg cgtataggtg actgcgggct
     1081 tatcgatatt gccgtggtac gttgctttcg gccctagcca gcgcactggc atattgccgt
     1141 cagcaaacag gccaagcagg ggtttttgct gattcgcttc cgtcaccgaa ttcagtgagg
     1201 cagcatcgtt caccaactga taaccacgcg cctgtgcctg ttcacgcagc gtttttccct
     1261 gccattcacc agcggttgcc gtttcagcaa aggtttttgc gccgccgcca agcgtaacgt
     1321 cggcacgagc gttaagcagc tgttcggtaa tcgatccttt tccgcctttt tccagagcgt
     1381 tacccggaca tttttcactg gtcgcgctcg gaccgtagca tttgcgcgaa gtcacatgtg
     1441 ccaccagcgc agcgggcgtg gcatcctgca actctgcggt agaaacgtta ccggtcgcca
     1501 gacctgcggc ttttgccatt tccagaatcg ttgggtgatc tttttcgtga atatcgacgc
     1561 ccagcgcgcc gttataggtt ttgacatcgg ttgaccaggc ggttgctgat gcagccgagt
     1621 cggtgacgta gtccggtttg ccagtttttt tattcagcgc atagtgagtg tattgcccgg
     1681 taagcggtaa ggcatcgata cctttaaaaa agccgcccgc accttcggca taattacgtg
     1741 cggcagtaat ttccgaatcc cccatcccat cgccaatcag caaaataata ttttttgcag
     1801 gtttatcgct aagagaatca cgcagagcgg cggtctgatc acccgttaag cggcgagcac
     1861 cgccgggtgc agtaatatcg ccctgagcag cccggttttc cagaacaggc atttctggtg
     1921 tccgggcttt tgtcacaggg gtaaacagta acggtaagag tgccagtgca atagtgcttt
     1981 gtttcacttt attttctcca tgtacaaata cattaaaaaa taaaaacaaa gcgactataa
     2041 gtctcggccg tgacaacttt atgacagctg ttgaaaagat taacttttta ctgacaagga
     2101 tgcttcaata acttctttac gtaatcgcgc agcagctctg tatcgtcgtc aggaatgctg
     2161 gcatcgggct ttacctcgta cagcgccccc tctacctgat caatcaaccg ctgttggtca
     2221 ttttgcgcca tattgcgaag cattgcagtg acgataatct ccaaagcttc tacctgcgca
     2281 cacagttctt tcgactcttc ttctttttgg gcaagcttaa ataacaactc agcaatgaga
     2341 tttttcatgt ctgtatttcc ttatccaaag tatggagaag ttaacattat gtttca
//
LOCUS       DAZPCS010000292         2384 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZPCS010000292 DAZPCS010000000
VERSION     DAZPCS010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.298"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..682
                     /locus_tag="ACHUQ7_004060"
     CDS             <1..682
                     /locus_tag="ACHUQ7_004060"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR1137213.1"
                     /translation="TPPLIAVIGSDASGKSTVCEHLITVVEKYGAAERVHLGKQAGNV
                     GRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLACRRR
                     GLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDLVIKL
                     NVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEKAITD
                     FMTARGYH"
     gene            complement(731..2230)
                     /gene="pitB"
                     /locus_tag="ACHUQ7_004061"
     CDS             complement(731..2230)
                     /gene="pitB"
                     /locus_tag="ACHUQ7_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HFR1137214.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPIDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            2167..>2384
                     /locus_tag="ACHUQ7_004062"
     CDS             2167..>2384
                     /locus_tag="ACHUQ7_004062"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1137215.1"
                     /translation="MPEQVITKAPYIYQGQQINLAYIRLRHFEFTNGRIISDFNGMGK
                     VKYHFLTIKRVLFLVYQTISIHSKPLEI"
BASE COUNT          653 a          608 c          566 g          557 t
ORIGIN      
        1 aacacctcca ttaattgccg ttattggtag cgatgcttca ggcaagtcaa cggtgtgtga
       61 acatcttatt accgttgtcg aaaaatatgg tgctgccgaa agagttcatt taggaaaaca
      121 ggccggaaat gtcggtcgtg cagtgacaaa attacctttg atgggaaaat ccttacataa
      181 aacaattgaa cgaaatcagg tgaaaacagc aaaaaaattg cctggaccag ttccggcgct
      241 ggtaattaca gcgtttgtcg cccgtcgctt actgcgcttt cgtcatatgc ttgcctgtcg
      301 tcgtcgcggg ttaattgttc taaccgaccg ttatcctcag gaccaaattc ctggcgctta
      361 cgatggtacg gtgttcccac ctaacgttga aggtggtcgt tttgtctcat ggctggcaag
      421 ccaggaacgt aaagcgtttc actggatggc gagccataag cctgatctgg tcatcaaact
      481 caatgttgac cttgaagttg cctgtgcacg taaacccgac cataaacggg aatcgctggc
      541 gaggaagatt gccataacgc cacagttaac ctttggtggt gcacaactgg ttgatatcga
      601 tgccaatcag ccactggaac aggtgttggt tgatgcagaa aaagcgatta cggattttat
      661 gaccgcgcgt ggttatcact agtcaaaaat ggaaatgccc gatcgccagg accgggcatt
      721 ttcaggaagg ttaaatcaac tgcaatgcta tccagtacag cccaccagaa agaaaaattg
      781 ccgccggtaa agtaaacacc cacgccatca ggatgctggt taccgtttta cgctgtaacc
      841 caccgccgtc caccaccatc gtccctgcta ccgcagacga gaggacgtgt gttgtggaga
      901 caggcatccc aatataactg gcaaggccga tagacactgc cgccgtcatt tgtgccgcca
      961 tgccttgcgc atacgtcatg ccgcgcttac caatcttctc accgatggtc atcgctacac
     1021 gacgccagcc aatcatggtg ccaatgccga gcgccagtgc taccgccatg atgatccaca
     1081 ccggagcgta ctcaatggtg cttaacatat cgctgcgcag ttttttcagc aggttctggt
     1141 cttctttact gacgcctggc agtttcgcca gcttcgcgga ggtatcggag atgcacagca
     1201 taatgcggcg cagctggctg cgctgactca cgcttaacgg ctcgtaactt tccatattgc
     1261 ctggcagcat cgttttaacg cgcgcaatcg catcaaaggt atttgccgga tgacagtgaa
     1321 actctgttac ttgcgtgcca tcagtcgatg ctgcaggcaa tggaggttcc atcgcaatca
     1381 acttctgcgg cagttcagga tgctgttgca ggtagtgttc gaagttggta acggcatcgc
     1441 gggtacgggt aatttcatag ccggacgcat tcatattgac gacgaagcca gcaggggcaa
     1501 tccccaccag taccagcatt accaggccga tccctttctg cccgtcgttc gcgccgtgcg
     1561 aaaacgccac gcccgcagcg gaaacaatca gcgcaatacg cgtccagaat ggcggtttac
     1621 gtttgccctt tttctttttg cgatcttccg gaatgcggtg aatacggtca cgctttttcg
     1681 tcccactcca gaaacggcgc agcaggaaaa tcagaccacc cgcaatgacc aggccgacga
     1741 taggggaaac aatcagcgag gagaaaattt tggttacttc acgcaggttt aacgcatcca
     1801 tcaccgatga gccggttaac agcgcgttgg ttaaaccgat gccgataatc gcaccaatca
     1861 aggtgtgcga actggaggcc ggtaaaccaa agaaccacgt tcccaggttc cagataatcg
     1921 ccgccagcag catggaaaag accatcgcca aaccgtgagt tgaccccata ttcagcaaca
     1981 aatcgattgg taacatatgg acaatggcat aggcaacgct aagtccgccc aataacacgc
     2041 caaaaaagtt aaaaaatgcc gccatcacca cagcaagttg tggctgcatg gcacgagtat
     2101 aaataacggt tgccaccgca ttcgccgtgt catgaaaacc attgattgct tcgtagaaca
     2161 acacaaatgc cagagcaagt aataacaaaa gccccgtata tatatcaagg ccaacaaata
     2221 aatttagcat atattagatt acgccatttt gaatttacga acggacgcat tatcagtgac
     2281 tttaacggca tgggcaaagt gaaatatcat tttttaacga taaaaagagt gttatttctt
     2341 gtgtatcaga caataagcat tcattcaaaa cctttagaaa taat
//
LOCUS       DAZPCS010000293         2377 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZPCS010000293 DAZPCS010000000
VERSION     DAZPCS010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.299"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(236..331)
                     /locus_tag="ACHUQ7_004063"
                     /pseudo
     CDS             complement(236..331)
                     /locus_tag="ACHUQ7_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(367..672)
                     /gene="ccdB"
                     /locus_tag="ACHUQ7_004064"
     CDS             complement(367..672)
                     /gene="ccdB"
                     /locus_tag="ACHUQ7_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HFR1137216.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(674..892)
                     /gene="ccdA"
                     /locus_tag="ACHUQ7_004065"
     CDS             complement(674..892)
                     /gene="ccdA"
                     /locus_tag="ACHUQ7_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HFR1137217.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
     gene            complement(1428..2377)
                     /locus_tag="ACHUQ7_004066"
                     /pseudo
     CDS             complement(1428..2377)
                     /locus_tag="ACHUQ7_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          567 a          631 c          571 g          608 t
ORIGIN      
        1 acaaatggct ttgttaaaag aaataagtag gagtttttat gaagttcatt ccatgaaatg
       61 aacttcagca cttttttatt ataataagga taaacaaaat tggctgtaaa aaaacgatac
      121 tgtttcagtt aaccgtgaat ggcctgtttg caccgtattg ttcttgtttt tcggttagtt
      181 aaacatcgta cttcatattt gaacggtaag cggctcgtca gaaccgtatt gatatttact
      241 gagaggtcag atcaactttc caaggcaaca gatcgcgtac ccggtttgcc ggccagtcct
      301 ggatatgctc aatgacgtaa cgcagccatt ttgtcaacgc cacgattttt gaccctagcc
      361 tgacatttat attccccaga acatcaggtt aatggcgttt ttgatgtcat tttcgcggtg
      421 gctgagatca gccacttctt ccccgataac gaagatcggc acactggcca tatcagtggt
      481 catcatgcgc cagctttcat ccccgacatg caccaccggg taaagctcac gggagacttt
      541 atctgacagc aggcgggcac tggccagggg gatcaccatc cgtcgtccgg gcgtgtcaat
      601 aatgtcactc tgtacatcca caaacagacg ataacgactc tctcttttat aggcgtaaac
      661 cttaaactgc atttcaccag tccctgttct catcggcaaa agagccgttc atttcaataa
      721 aacgggcgat ctcagccatc ccctgctgat tttcagcctg ccagcgttcc gcacgcagac
      781 ggcgggcctc tttttgcatg gccgtattta ccaggcctga gatattcacg tttgcggatt
      841 taagtaactg atagctgtca ctgtctatag tgacagtaat acgctgcttc atgataagcc
      901 ctcaaaaaca tacttatgat atgtatatga gtatatatta atggtacaaa ccatcaatat
      961 ccataaaaag tacaccatta attcgctata agtttttaat acaatttttt ctgctgcgat
     1021 ttttttatac tataattagc atacttataa caccgtaagt aaattacatg aaagcaggca
     1081 cagcgtgatc cgatttttct gcgtataacg attttccgga agtgtaatac ggttgcgccc
     1141 cgggccgggc acccttttct gttgttgtgt acgggcccac agagaatggt accggaggcg
     1201 ggctcagaag tactgagtcg ggtgatcgcg tcaggccgcg aagtactgac acgggtattg
     1261 gtgactggtg atgaagtact gccccgggta tgatttcagg ctgattcagg ttctgattct
     1321 ctgtcagtcc gcgtggttgc tggcctggct gatttcctgt tgtcaggcgt tcaacaaccg
     1381 actgcaggtt atagcccggt tcacgaacac ctgacggcag gtatcactca cccggtgaag
     1441 gtaaatccct caagaggcag caactctgcc agtcgctcct ccggccattc cggcagacgc
     1501 gtcaggacgt ccgtcagcca ggcatgtggc tccagaccat tacgttttgc tgtttccaga
     1561 aggctcatta tctgtgccgc ccggtttccg gccatcagtg aaccggcgaa cagccacgct
     1621 ttccgtccca taacaaccgg gcgaatggca cgctcgcaga tattgttgtc caggggcaca
     1681 gctccgtcac ccaggaagca gctcagttca tcgcgccggt tcagaatata gttaatcgcc
     1741 ttatttaatg gcccgtcggg cggacagcac ggctcctgct cttcaagcca ggcaaacagg
     1801 tcattcacta tcggccggga gtaccgttat cgccactgcc gtattttttc cacagggcgc
     1861 tcacggatga gtttctcaat gcggtaaaga cccgcgattt ttctcagctc cacgcctgca
     1921 cggggctccc tgctggcctt atacagattt gcgaagccgc gccctgcgtg actccagcaa
     1981 caggctgatg tgatgccggg gtgattatcc gccagagatt tgtacacgct gtatccgtca
     2041 ctcaccagcg tactgccaca ccagcactgc agccaggtct cagggtatct gagggcccgt
     2101 ccggtctgcg agtcaaaaca gacgaccggc ggaccgcttc tttcaccgct gacgtaagca
     2161 cacagccagc cggagcagga tttaccgcct ttccgggtat caaggagcct cagggacgtt
     2221 tcatctgcat gaataacatc ccgtgtcaga agctcatggc gtagcagttt cgccagcggg
     2281 gccagtgcag ctccggcaac accgaccata tccgccattg tactgacggg gagctccaca
     2341 cccattcggg agaaaatgtg ctgctggcga tacaggg
//
LOCUS       DAZPCS010000294         2325 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZPCS010000294 DAZPCS010000000
VERSION     DAZPCS010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.300"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            61..282
                     /gene="kil"
                     /locus_tag="ACHUQ7_004067"
     CDS             61..282
                     /gene="kil"
                     /locus_tag="ACHUQ7_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707273.1"
                     /note="Rac prophage Kil protein; Kil overexpression leads
                     to morphological defects and inhibits cell division;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFR1137218.1"
                     /translation="MIAHHFGTDEIPRQCVTPGDYILHEGRTYIASANNIKKRKLYIR
                     SLTTKTCISDCMIKVFLGRDGLPVKAESW"
     gene            282..452
                     /locus_tag="ACHUQ7_004068"
     CDS             282..452
                     /locus_tag="ACHUQ7_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaE family protein"
                     /protein_id="HFR1137219.1"
                     /translation="MTKKIKCAYHLCNKEIEESKIITRPLHFMRGVIPTTEMKKYCSE
                     ICAEKDQMAHEL"
     gene            527..802
                     /locus_tag="ACHUQ7_004069"
     CDS             527..802
                     /locus_tag="ACHUQ7_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1137220.1"
                     /translation="MITSYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLNDGVKKMCLVHIGKNLPAEKKAEFLATLIAMKLKGEI"
     gene            902..>2325
                     /locus_tag="ACHUQ7_004070"
     CDS             902..>2325
                     /locus_tag="ACHUQ7_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
                     /protein_id="HFR1137221.1"
                     /translation="MNTDKQVYPLYYEAKNDKVRKRLGIKGGFYWAEAKKLSIAISRG
                     AVAIDDAGYDEDDFKKPVRVNLPVVDDLPPEGVFDTEFCNRYEKGGEDGITMVFIASS
                     PSVQDKPASTDNTNVNGEDMTEIEENMLLPVSGQELPIRWLAQHGSEKPVTHVSRDEL
                     QALHIARDEELPAVTALAVSHKTSLLDPLEIRDLHKLVRDTDRVFPNPGNSSLGLMTA
                     FFEAYMDADYTDRGLLTKEWMKGNRVSRITRTASGANAGGGNLTDRGEGFVHDLTSLA
                     RDVATGVLARSMDVDIYNLHPAHAKRVEEIIAENKPPFSVFRDKFITMPGGMDYSRAI
                     VVASVKEAPIGIEVIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTEE
                     GKQDDEEKTQPSGAMADEQATAETMEPDATEHHQNTQPLDAQSQVNSVDEKYQELRAE
                     LHEARKNIPPKNPVDADKLLAALR"
BASE COUNT          720 a          531 c          528 g          546 t
ORIGIN      
        1 taagtattaa ataactttcc tgagatttcc tttgtaagac tgtttattaa ggaggcttcc
       61 atgattgctc atcacttcgg aactgatgaa ataccacgtc agtgtgtgac tcctggcgat
      121 tatattcttc atgaaggtcg gacatatatc gcctcggcaa acaatattaa aaagcgaaaa
      181 ctttatattc gtagcctgac tacaaaaaca tgcatttctg actgcatgat taaagtcttc
      241 ctcggtcgtg atggtttacc tgtaaaggcg gagtcatggt aatgactaag aaaataaaat
      301 gtgcttatca cctttgcaat aaagaaattg aagaaagcaa aatcattaca agaccacttc
      361 atttcatgcg tggagttata ccaacgacgg aaatgaaaaa atattgtagt gaaatctgtg
      421 ccgaaaaaga ccagatggca cacgaacttt aattaactga ctatccgaaa ctgaatttat
      481 gccagcaatg gcagggattc gctcaacctt aattaaggag aaaaacatga ttaccagtta
      541 tgaagccact gttgtaacta ctgatgacat tgttcacgag gttaatctgg aaggaaagcg
      601 tattggctac gtgattaaga cagaaaataa agaaacccca ttcactgtgg ttgatatcga
      661 cggtccatca ggcaacgtta aaacacttaa cgatggtgta aaaaaaatgt gtctggtgca
      721 cataggaaag aatctgcccg cagaaaaaaa agccgaattt ctggcaactc tgattgcaat
      781 gaaattaaaa ggtgaaatct gaaaaaaaga aagcctgcac actgtgcagg cctgaatgaa
      841 gaacctggga catttattca tcactcgcag taattttaat ctgagttgag gttaaaaaac
      901 aatgaacacc gataaacaag tttacccact gtattacgaa gcaaaaaatg acaaagtaag
      961 aaaacgtctc ggtattaaag gcggttttta ctgggctgaa gcgaaaaaat tatccattgc
     1021 catctcccgt ggtgctgttg cgattgacga tgcaggctac gatgaagatg actttaaaaa
     1081 acctgttcgc gtcaatttgc ccgttgttga tgacctcccg ccagaaggcg tatttgatac
     1141 ggaattctgc aaccgttacg aaaaaggcgg ggaagatggc atcacaatgg tatttatcgc
     1201 atcttctccc tctgttcagg acaaaccggc cagcactgac aataccaacg tcaacggcga
     1261 agacatgaca gaaattgagg agaacatgct cctgccagtt tccggtcagg aactgcccat
     1321 tcgctggctt gcgcaacacg gcagcgaaaa accagtaacg cacgtttcac gcgacgaact
     1381 ccaggcatta cacattgctc gggatgaaga actaccagct gttactgccc tggctgtttc
     1441 ccacaaaacc agcctgctcg atccgctgga aattcgcgat ctccacaaac tggtgcgtga
     1501 tactgacaga gttttcccta atccaggcaa ttcaagcctg gggctgatga ctgctttttt
     1561 cgaagcatac atggacgcag actacaccga tcgcggtctg ctgacaaaag agtggatgaa
     1621 aggaaatcgt gtttcacgca tcacgcgcac ggcttccggc gctaatgctg gcggcgggaa
     1681 cctcaccgat cgcggcgaag gtttcgttca cgatctgacg tcactggcgc gcgacgtagc
     1741 cactggcgta ctggctcgtt caatggacgt ggacatttat aaccttcatc cggcacacgc
     1801 taaacgtgtc gaggaaatta tcgctgaaaa taaaccgccc ttttctgttt tccgcgacaa
     1861 attcatcacc atgcctggcg ggatggatta ttcccgcgcc atcgtggttg cgtcagtgaa
     1921 agaagcaccg attggtatcg aggtcatccc cgctcacgtc actgaatatc tgaacaaggt
     1981 actgactgaa accgatcatg ccaaccctga tccggaaatc gtggatattg cctgcggtcg
     2041 ctcctctgcc ccgatgccgc aacgtgtaac agaagaagga aaacaggatg atgaagaaaa
     2101 aacgcagcca tctggcgcaa tggcagatga acaggcaacg gctgaaacaa tggaaccgga
     2161 tgcaactgaa catcatcaga acacgcagcc gctggatgct cagtcacagg taaattctgt
     2221 tgatgagaaa tatcaggaac tacgggcaga actccatgaa gcccggaaaa acattccgcc
     2281 caaaaatcct gtcgatgcag acaaattact ggctgccttg aggta
//
LOCUS       DAZPCS010000295         2294 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZPCS010000295 DAZPCS010000000
VERSION     DAZPCS010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.301"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..414
                     /gene="torD"
                     /locus_tag="ACHUQ7_004071"
     CDS             <1..414
                     /gene="torD"
                     /locus_tag="ACHUQ7_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFR1137222.1"
                     /translation="TLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQEIKR
                     LLVEAGMETSGNFNEPADHLAIYLELLSYLHFSLGEGTVPARRIDSLRQKTLTALRQW
                     LPEFAARCRQYDSFGFYAALSQLLLVLVECDHQNR"
     gene            complement(507..812)
                     /gene="cbpM"
                     /locus_tag="ACHUQ7_004072"
     CDS             complement(507..812)
                     /gene="cbpM"
                     /locus_tag="ACHUQ7_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFR1137223.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(812..1732)
                     /gene="cbpA"
                     /locus_tag="ACHUQ7_004073"
     CDS             complement(812..1732)
                     /gene="cbpA"
                     /locus_tag="ACHUQ7_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFR1137224.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            1993..>2294
                     /locus_tag="ACHUQ7_004074"
     CDS             1993..>2294
                     /locus_tag="ACHUQ7_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1137225.1"
                     /translation="MGSNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVS"
BASE COUNT          592 a          548 c          563 g          591 t
ORIGIN      
        1 acgctgacag tacgtgacga tgcccgtctg gaactggccg ctgacttttg cggcctgttt
       61 ctgatgaccg acaaacaagc ggcgctgccg tatgcatcgg cctacaaaca ggacgagcaa
      121 gagattaaac gcttgttagt tgaggcaggg atggaaacca gtggcaattt caacgaaccg
      181 gcagatcatc tggcgatcta tctcgagttg ttgagttatc tgcatttttc gctgggagag
      241 gggaccgttc ctgcgcgaag aatcgacagt ttgcggcaaa aaacactgac ggcgctgcgg
      301 caatggttac cagagtttgc tgcgcgttgt cgacagtatg acagctttgg tttttacgcg
      361 gcactaagcc agttattgct ggtgttagtg gagtgcgacc accaaaacag ataacgtcgt
      421 ttatgcgcct gaaaagacgc gtttagcgtc gcatcaggca ttatggcgca gttgccggat
      481 gcgtcatcac atccggcaaa cacacatcac ggatgagcta caaaccggga aagccgctgg
      541 cgtagcaggc ggttttcctg cttcaggtgc gcaatatcat ccattaacgt cagcgccacc
      601 gcgatccccg gccagtccag agccagttca tgacgcaggc gtaccgcgcg ttgcaccaca
      661 atggcggcat ggtcgtcaaa tacccaggtt gtttcctgga tctcacgcgg ttcaaccacc
      721 cccaaaccga caatttcatt caactcctct tcagagatgc cggtatgcag gcaaaattcg
      781 gtaatagtaa aagtcaccgt aacattagcc attatgcttt cccccaatct ttacgtggat
      841 caaaagacga ctgggcgtct gccagttgct gccacagcgc ggcagtgttt tcatccggtt
      901 tcggcggcat cacgattttc agtaccgcat acagatcgcc ggtctgtttt ttgctcacca
      961 gacctttgcc tttaacgcgc aatcgttgcc cggcctggct gcctggcggg atagtcagca
     1021 aaatgctttc tttcagtgtt ggaacggtga ctttagtacc cagcgccgct tcccacgggc
     1081 taaccggcac cacaatttcc agatcctggc cgacaatatc gaacagcgga tgtggcgcaa
     1141 tatgaatcac cagccacaaa tcgccatttg gaccgccgtt ttcgcccggc gtcccctggc
     1201 ctttcaggcg gatgcgttga ccattgccga cgcctgccgg gatcttcaca ttcagcgttt
     1261 tcgggatttc ctgttcgatc atgccaaagg cgttataaac cggcaggtta tagctgatgg
     1321 tacgcttatg ctcagtaagc gtttcttcga ggaataccgc cacttcgatt tcaatatcgt
     1381 ggccacgtgc ggcggggcgt tgacggctct ggcgggcatg ctgaccgaaa attgacgaga
     1441 agatatcgtc aaaatcttcg gcattaaaac tctgaccgtc gccatgatgg aactgacggt
     1501 taaattgcgg atcgttgcga tgttgccaca tctgatcata ctcagcgcga cgttgttcat
     1561 cacttaacac ttcccaggct tcagcgacct ctttgaagcg ggcttcggca tccggttctt
     1621 tgctgacatc aggatggtat ttacgggcca gtcgacgata ggcggtcttg attgtcttga
     1681 gatcgtccgt cggtttcacg cccatgatgg cgtaataatc ctttaattcc atagcgttat
     1741 ctcgcgtaaa tcaacacaaa ttgaaggaac ccctgtaagg taactcctat aagtgtaggg
     1801 taatcctcaa aatttcatat gccaacacag aatatgttat tgaaatcatc gcggagagga
     1861 ggtcgccatc aagatgggtt gctgaacata ttttaaacag gtgaaaaagg gtgagcgatt
     1921 tttgatagtt gaaccaggca ctttaagttt aactagggcg tcattattta ttaaatttta
     1981 tagacgctat atatgggtag taatatacat ggaattagtt gcactgcaaa taattatttg
     2041 aaacaggcct ggaacgatat aaaaaatgag tacgaaaaaa atcaaacata ttcaatcacg
     2101 ctttttgaaa acacactggt gtgttttatg cggttgtaca atgaactcag acgtaaagta
     2161 aatgaagagg atactccatg tctggaatgt gaatcactag aaaaagaatt tgaggaaatg
     2221 cagaatgata atgatctatc attatttatg agaacattgc gtactaatga tacacaaatt
     2281 tattcagggg tttc
//
LOCUS       DAZPCS010000296         2269 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZPCS010000296 DAZPCS010000000
VERSION     DAZPCS010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.302"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..92
                     /locus_tag="ACHUQ7_004075"
     CDS             <1..92
                     /locus_tag="ACHUQ7_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFR1137226.1"
                     /translation="VLGIRDGIISDQTALRMLQEYIRIQCLGG"
     gene            complement(181..693)
                     /locus_tag="ACHUQ7_004076"
     CDS             complement(181..693)
                     /locus_tag="ACHUQ7_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFR1137227.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            888..1652
                     /locus_tag="ACHUQ7_004077"
     CDS             888..1652
                     /locus_tag="ACHUQ7_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1137228.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLDWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            1603..>2269
                     /locus_tag="ACHUQ7_004078"
     CDS             1603..>2269
                     /locus_tag="ACHUQ7_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HFR1137229.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDMLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAA"
BASE COUNT          649 a          495 c          573 g          552 t
ORIGIN      
        1 acgttctcgg tatccgggac ggaatcatca gcgaccagac agcattgaga atgcttcagg
       61 agtacatcag gatccaatgc cttggggggt agcggtaatt ttactcatta tccttcaaat
      121 cagattctgt tgtcagagga atgggggaag ctggattcgc aagagctttt tatgggcagt
      181 ttagctaaaa aaaggcaaca cgaagctaaa actaccaaca taaagtgctg accagctagc
      241 agctgaaatc agactcactg tatacacttt cactggatga agttgcaaca ttcctgccac
      301 caaaggagca atgtaacgca gcacggcgat aaagcgcgaa gtgaaaagta ttgacacaga
      361 gttattttgc aactgaaggc gaacccgttc tattgtattg gaacggctcg atattataca
      421 tgcaagttga gggatatgcc caattattat accaagatgg taattaacga tagtaccgca
      481 ccatgcgcca ctcatgaccg tgattccagc ttcccatggt gaaagagtcg tcttgctgac
      541 tgtaattaca gtggccatca taattgatgc tggtggcaat atcgccgaaa tgagtaaagt
      601 ggattttgtg aacgctatta taaaaagaag cccccatagg cgatatggat gaagtacaaa
      661 atagttcatc aaagcattta tccactccat caatgtcttt ccttctgtga atttttcttt
      721 gctatttaac cgtgatacga cttaatttag gcttaaacac cttacattta caaggtactc
      781 ctgtgggggg ccctgccacg gagcgtcgca ggagtgagtg atgaggaaga attaaagcaa
      841 cctattgtga caggcagcga taacagtaaa tcacaggaag cataatcatg gcaaaaccgg
      901 actgggaggc catcgaatcg gcataccggg ccggagtcct tagcctccgt gatataggcg
      961 agaaatacgg cgttacagaa ggggctatca ggaagagggc caaaaagctt gattgggcac
     1021 gcagtggcgg tacgcaggtt tgcaaaaatg gtacgcaaaa aaggaaagtg cgtaccagca
     1081 gaaagcctgc cattactggc cttacacaaa aaagtacgca actaaaaaca gaatctacac
     1141 cggatacgaa accgatacgc ggaatgcgta ccgatccccc gactaaccca ttccaacccg
     1201 gtaaccagca ggcattaaaa cacggtggtt atgcccgtcg ccttctgctc aaagatgagg
     1261 tgatagagga cgctaaagcg ttgacgctcg aggacgaatt atttcgcctt cgtgctaaca
     1321 accttgtcgc tgcagagaat attggtcggt ggttggtgtc gctggaagat gctaatgggg
     1381 accaggaaag gaagatgctg attgaaaata tcagcgccgc cgagaaagca atgatgcgca
     1441 atacagttcg tattgagtcc atcgttggca cgcttgcgac ggtaggaaaa atatttgctg
     1501 atacagccta ccgcaaggcc gccactgata aggtgtctct ggaggctgat cgtcttcgcc
     1561 gtgatgcagg tattgatgat ggcaatggag agcgtgacct caatgacttc tactctgaca
     1621 tccaaaccga cgctgaatcc ggctttacgt agtttctgga ctatgcgggc acgtaacaaa
     1681 gtgctttatg gtggtcggtc atcgtcaaaa tcatgggatg ccgctggcat tgccatattt
     1741 ctgtcgaata aatacaccct gcgtttttgt tgtgcccgtc agatccagaa taaaatcgaa
     1801 gagtcggtgt ataccctgct caaaattcag atagacaggt ttggtctgcg gcaccgtttc
     1861 cgtattctga acaacaaaat cattaaccgg gttactggct cggaatttgt tttttatgga
     1921 ttatggcgca acatcgaaga aattaagtca ctggagggga tcgatatgtt gtggttggaa
     1981 gaagcccacg cactgacgga ataccagtgg aaaattctgg agccaacgat ccgtaaagag
     2041 ggttcggaat gctggttcat attcaacccc ggacttgtta ctgatttcgt ctggcgcaac
     2101 ttcgttgttg atccgcccga aggcactctc atccgcaaaa ttaactatga cgaaaatccg
     2161 tttctgtctg acaccatgct taaggttatc gacgcggcgc gacgccgtga tccggatggt
     2221 tttaaacatg tgtatgaggg cgttccggag tctgatgatg atgcggcaa
//
LOCUS       DAZPCS010000297         2263 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZPCS010000297 DAZPCS010000000
VERSION     DAZPCS010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.303"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..1057
                     /locus_tag="ACHUQ7_004079"
     CDS             <1..1057
                     /locus_tag="ACHUQ7_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFR1137230.1"
                     /translation="ANVAHAAEVYNKDGNKLDLYGKVTALRYFTDDKRDDGDKTYARL
                     GFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAGLKISEFGSLDYGRNY
                     GVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDFFGLIEGLNFAAQYQG
                     KNKRTDNSHLYGADDTRANGDGFGISSTYVYDGFGIGAVYTKSDRTNAQERAAANPLN
                     ASGKNAELWATGIKYDANNIYFAANYDETLNMTTYGDGYISNKAQSFEVVAQYQFDFG
                     LRPSLAYLKSKGRDLGRYADQDMIEYIDVGATYFFNKNMSTYVDYKINLIDESDFTRA
                     VDIRTDNIVATGITYQF"
     gene            1221..1706
                     /locus_tag="ACHUQ7_004080"
     CDS             1221..1706
                     /locus_tag="ACHUQ7_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR1137231.1"
                     /translation="MIMTNINTACVKNNASYQLNNALPNKETISSNFCERLEQWGNKS
                     VNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLA
                     DNASLVNINLSVNKINTITFPKEPKLENIYINHNNLESLDLKNQHSLVNLEAQNNSLK
                     K"
     gene            1816..>2263
                     /locus_tag="ACHUQ7_004081"
     CDS             1816..>2263
                     /locus_tag="ACHUQ7_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR1137232.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQG"
BASE COUNT          735 a          475 c          419 g          634 t
ORIGIN      
        1 tgcgaacgta gcacacgctg ccgaagtcta taacaaggat ggtaataaac tcgaccttta
       61 tggcaaggtt accgctctac gttattttac tgatgataag cgtgacgatg gtgataaaac
      121 ttatgcccgt ctcggcttta aaggagaaac gcaaatcaat gatcaaatga ttggttttgg
      181 tcactgggaa tatgatttta aaggctataa cgatgaagcc aacggctcgc gcggcaacaa
      241 gacccgtctg gcctatgctg gtttaaaaat tagtgaattt ggctctctgg actatggtcg
      301 taactacggt gtcggctacg acattggttc atggaccgat atgttgccag aatttggtgg
      361 cgatacctgg agtcagaaag atgtcttcat gacataccgt accaccggtc tagcaaccta
      421 tcgcaactac gatttctttg gcttaattga agggctgaac tttgccgcgc aatatcaagg
      481 caaaaataaa cgtactgaca acagtcatct ttatggtgct gacgacacgc gtgccaacgg
      541 tgacggtttc ggtatctcct caacttatgt ttatgatggc tttggtatcg gtgcggtata
      601 taccaaatcc gatcggacaa atgcgcagga aagagccgca gctaatcctc tcaatgcctc
      661 cggtaagaat gcagaactat gggccacagg tataaagtat gatgccaaca acatctactt
      721 tgcagctaat tacgatgaaa cattaaacat gaccacctat ggggatggtt atatctctaa
      781 caaagcacaa agttttgaag tggtggcgca atatcaattc gacttcggct tgcgcccatc
      841 actcgcttac ctgaaatcga aaggcagaga tctgggccgc tacgccgacc aggacatgat
      901 tgagtatatc gacgttggtg cgacgtattt cttcaacaaa aatatgtcga cctatgttga
      961 ttataaaatc aacctgattg atgaaagcga ctttacccgt gccgtagata ttcgcaccga
     1021 taacatcgtc gctacgggca ttacctatca gttctaacgt cctctcctga ccagcccttc
     1081 tctgggctgg tttttgattt aagtgctgct gtatgactcc ttaagtaaaa atgagcaatt
     1141 cttgatattt ctggtcattc acctcaatta atattattcc atatcctccg gggacatctt
     1201 actataatat ttattgatga gtgattatga ccaatattaa tacagcttgt gtaaaaaata
     1261 atgccagtta tcaacttaat aacgcattac ctaacaaaga aaccatctcc agcaattttt
     1321 gtgaacgact agaacaatgg ggtaataaat cggttaataa tggtgaagaa agagcaattg
     1381 ccgtggagcg aattaaagaa gcttacaatt cgaacatggc atctcttgat ttgtcatatc
     1441 ttgatttgag tgaattaccg cctatcccct cgacagttaa tacgttaaat ctggaaaaca
     1501 attgtctaac ttgtcttgac ttagctgaca atgccagtct cgttaatatc aacctcagcg
     1561 ttaataaaat taacacgata acctttccaa aagaaccaaa actggaaaac atttatatta
     1621 atcataacaa tctggaaagt ttagatttaa aaaatcagca ttcattggtt aatctggaag
     1681 cgcaaaataa tagcctaaaa aaatgatatt tctgatagtt ataaactgaa atttcttaat
     1741 cttgattata acaagctagc atcgctggat gtctcccggc aagaatctct gattgagtta
     1801 agtgcccatc acaatatgat cactgacctt atattacaca atcatcccag gatgaaaaca
     1861 atcactttaa acgacaacca tattgcacat ttaaacgcca aaaacactac aaaactggaa
     1921 tatttaaact taagcaataa caatttactg ccaaccaatg acattgatca actaatatca
     1981 tcaaaacatc tttggcatgt attagttaac ggcatcaaca atgatccact tgcacaaatg
     2041 cagtactgga ctgcagtaag aaatataatt gatgacacta atgaagtgac cattgattta
     2101 tcaggactta atttaaccac tcaaccacca gggctgcaaa acttcacctc tatcaatctt
     2161 gataataacc aactcacaca ttttgatgca accaactacg atagactcgt aaagctaagt
     2221 ctgaatagta atgctcttga gtcaataaat tttcctcaag gca
//
LOCUS       DAZPCS010000298         2262 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZPCS010000298 DAZPCS010000000
VERSION     DAZPCS010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.304"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            155..916
                     /locus_tag="ACHUQ7_004082"
     CDS             155..916
                     /locus_tag="ACHUQ7_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFR1137233.1"
                     /translation="MKNFNVLTKVVFCLSFLSSAFPTYAADDVKVTQNTSVFKLKLSA
                     TRLIYEQGSDGAAITVTNPQDWPMLVQSKVFEEDKVTAAPFIVTPPLFRLNPGQQNKM
                     WVVQTAPALQDREKLHWLCATGITPKKSDVWAEKDKEKIAENVNLNIQVNVHTCIKLI
                     TRPSGIEGKMVNASEKLSWKKQGDKIVAVNQSPFYINLSSLQVGGSKIEKLDYIAPFS
                     QHEFVMPKTSGQDIKWSVITDEGGESRLFISHLQS"
     gene            990..2262
                     /locus_tag="ACHUQ7_004083"
                     /pseudo
     CDS             990..2262
                     /locus_tag="ACHUQ7_004083"
                     /inference="COORDINATES: protein motif:HMM:NF012786.4"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
BASE COUNT          670 a          401 c          542 g          649 t
ORIGIN      
        1 ataaaagaaa taacaaaatt aagaaaagcc atatttacga tatgggcaac ataaatataa
       61 tctaaaaaag agtaaggcgt tgtaggttgg ttccgcgttc attaccttgt tagtgaacgc
      121 ggatttatca gttgtcgaaa tggtttttat aataatgaaa aattttaatg tactaaccaa
      181 agtcgtattt tgcttgtcgt ttttatcaag cgcatttccc acctatgctg cggatgatgt
      241 caaagtgact caaaacactt cagtttttaa actgaagctt agtgcaacgc gattaatcta
      301 tgaacaaggg tcggatggcg cagcgataac tgtgacaaat ccgcaggatt ggccgatgct
      361 tgtgcagagt aaagtctttg aagaagataa agtaactgca gcgcccttta ttgtcactcc
      421 gccactgttc aggcttaatc caggccagca aaataaaatg tgggttgtgc agactgcgcc
      481 agctttacaa gacagggaaa aattacactg gctatgcgcc accggcatta cgcctaaaaa
      541 gagtgatgtt tgggccgaga aagataaaga aaaaattgct gaaaatgtga acctgaacat
      601 ccaggttaac gtacatacct gcataaaact tattaccagg ccatctggta ttgaaggcaa
      661 gatggttaat gcgagtgaga agttatcgtg gaaaaagcag ggtgataaaa ttgtcgcggt
      721 caatcagtcg ccgttctaca tcaacctttc ttctttacag gttggcggta gcaagattga
      781 gaaactagat tatattgcgc cgttttcgca acatgagttt gttatgccaa aaactagcgg
      841 ccaggatatt aaatggtctg tgattactga tgaaggtggt gagagtcgtt tgtttatatc
      901 tcatttgcaa tcataatgta ttacattttt attgaatatt tttatgccat gcatattcca
      961 tgttttatcg actgtcatta attttgataa tggccttact tgcgggtcag ttaagtgccc
     1021 aggagtggtc atttgattct tcacagttgg aaggaaacgt tagtgcggac actgtggcaa
     1081 tgtttaatca gggagagcaa ctccccggaa attatcgtgt ggaaatttat cttaacggtg
     1141 aaaaagttga tgttggggag tttccctttc atcgtccgga atcccctgaa gaaaaagagc
     1201 ttgttccatg tttaaccgtt gatgacctta tccactatgg cattaaaatt gataaaagca
     1261 gcagtgatac tgacaataaa aagaatcagt gttttaaatg gaactctatt gaaggtttga
     1321 aagtaaatta tgattttgat agccagcgcg tacagataac cgttccacaa ctgtatctgc
     1381 aagataaaaa aagtagttta gcaccagtgt cgttatggaa tgagggtgta gctgcattcc
     1441 gcatggtgta tcagacaaat attgatattt ccaaacaaaa tgataatcaa tctaccacgc
     1501 gtaattctcg ttatggcagg ttcactccag ggtttaatct tggtgcctgg aggtttcgta
     1561 gttcagtgac atggagcaag gaacttggcc agtcagaaag atggcaaagg ggttacatgt
     1621 ggtttgagcg aggcataaat gcgattaaaa gccgattaac gcttggagaa tcttatacgt
     1681 cgtctgaggt gtttgacagc ataccttttc gcggcgggat gttagcgacc gacgatgcga
     1741 tgactccgcc ggaggacagc tactacacac ctgtggtgca tggaatagca caaagtgagg
     1801 cgcaggttat tattaaacaa aatgggcaaa ttatttttac ccgttctgtg ccgccagggc
     1861 cttttgcgct ggataattta ccaacgctgg ccgttggcgg ggagcttgat gttacagtga
     1921 gagagagtaa tggcgaggag cagtattttt ccgtgccatt tcaaacgccg gccatcgctc
     1981 tgcatgaagg atatttcaaa tatagtgtga tgggggggaa tataaagaag aaggtataaa
     2041 aagctcccct gtctccgtgg gcgaaatcac ggcgatgtac gggcttcctt atggcgtcac
     2101 ggtgtatgga gggattcaaa acgccactca ttttaatgca atatctacgg gtattggcat
     2161 ttctctgggg ctgttaggga gtttatcgac agatattacg cgttcgattg cgaatttata
     2221 ttatgggaat aaatatcgta ttcgttatag taaatccatt ag
//
LOCUS       DAZPCS010000299         2251 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZPCS010000299 DAZPCS010000000
VERSION     DAZPCS010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.305"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..331)
                     /locus_tag="ACHUQ7_004084"
     CDS             complement(<1..331)
                     /locus_tag="ACHUQ7_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1137234.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
     gene            493..549
                     /gene="ysgD"
                     /locus_tag="ACHUQ7_004085"
     CDS             493..549
                     /gene="ysgD"
                     /locus_tag="ACHUQ7_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFR1137235.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            701..1651
                     /gene="corA"
                     /locus_tag="ACHUQ7_004086"
     CDS             701..1651
                     /gene="corA"
                     /locus_tag="ACHUQ7_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFR1137236.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1692..2072)
                     /locus_tag="ACHUQ7_004087"
     CDS             complement(1692..2072)
                     /locus_tag="ACHUQ7_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HFR1137237.1"
                     /translation="MSKEYMNDDSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(2086..>2251)
                     /gene="yigG"
                     /locus_tag="ACHUQ7_004088"
     CDS             complement(2086..>2251)
                     /gene="yigG"
                     /locus_tag="ACHUQ7_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YigG"
                     /protein_id="HFR1137238.1"
                     /translation="KIYVFGTLAVYIISIITMIAEDFACSGNGSMIFLICYFSTFSML
                     MLAPTDSSKQ"
BASE COUNT          591 a          529 c          521 g          610 t
ORIGIN      
        1 tctgctgacc gttttcgatg tacaaaccga gcggcgcata gctttcatca tagatgccgc
       61 cgttcatcgc catctgcacc tgaccctgac tattaatatc cgccagcaga gcatgtaacg
      121 ttccccacgc ttcgccattg gctttttgcc agtacatttt cacccgctct gtttgaggat
      181 taacggtata cgcctgtacg gtcagcgtcg gatctgagag tgcgcaatca tcagcggcaa
      241 cagcaaacaa gggaagtaag gtgagggtga gaaaaatccg tttgagattc aaggtgatca
      301 ttcctttacc aatgagtagc cgatgcgcca ttataggtcc tagatgtggg atttttttat
      361 cctgttagcg accttgacga gtaccaaaaa gcgcgaagtt caactattgt tctgtggtgt
      421 tctgttgcgt gttgacggca aaattttgct ggcgtaacat gcgcgcacga tcactctaag
      481 aggacattcg ccttggacac acccagtaga tactggctca ctatcctgtc atccaggatc
      541 aactcctaag gctatccctt tttgctgata gccttagcgg ttgtcagcga cctcaatttt
      601 tcccgtcgcg ctgagtcagg ctgtttaatg gtctgaaacc caatttgttt ctgtgtgccc
      661 accgaactgt ccgatatttt aagcattggg agtcccggtc atgctgagcg catttcaact
      721 ggaaaataac cgactgaccc ggctggaagt cgaagagtca caaccccttg taaatgcagt
      781 atggattgat cttgtcgaac cggacgacga cgagcgactg cgcgtacaat ctgaacttgg
      841 ccagagcctg gcaacccgcc cggaactgga agacatcgaa gcatcggcac gtttctttga
      901 agacgacgac ggcctgcata ttcactcctt cttcttcttt gaagatgcgg aagatcacgc
      961 cggtaactcc actgtggcat ttaccatccg tgatggtcgt ctgtttactc tgcgtgagcg
     1021 tgaactgccc gcttttcgtc tgtatcgtat gcgtgcccgt agccagtcga tggtagacgg
     1081 taacgcctac gagttgctgc tggatctgtt cgaaaccaaa atcgaacagt tggcagatga
     1141 aattgaaaat atctatagcg acctggagca gttgagccgg gtgattatgg aagggcatca
     1201 gggcgatgag tacgacgagg cgctctccac gctggcggaa ctggaagata tcggctggaa
     1261 agttcgcctg tgtctgatgg atacccagcg cgctctcaac ttcctggtgc gtaaagcgcg
     1321 tttaccgggt gggcaactgg agcaggcgcg tgaaatcctg cgcgatatcg aatccctgct
     1381 gccgcataac gaatccctgt tccagaaggt gaacttcctg atgcaggcgg caatgggttt
     1441 tatcaacatc gagcagaacc gcatcatcaa aattttctcg gtggtatccg tcgtgttcct
     1501 gccgccaacg ctggttgctt ccagctacgg gatgaacttt gagtttatgc cagaactgaa
     1561 gtggagtttc ggctaccctg gcgcgattat ctttatgatc ctcgcgggcc tggcaccgta
     1621 tctgtacttt aagcggaaga actggttgta aaaagaaggc ggtggcttag cctggctaag
     1681 ccacctgtta ttcaaaggct ccaggtattt aaccctttta cctctttctc ataaaaccat
     1741 ttgttcgtgt taacagcaac ataggctgct acggcaattc ccagaatgtt aacgccaatt
     1801 aatgcaccaa caaatagaga tagaatgcca agtaatagaa ctataattgc ttttttccat
     1861 aagcccagaa caaataaata gatccaggaa cagaagaaag caatgaaatt catttgaata
     1921 gttaatcgtt gtcttacttt taatgcttta aatgctgctt tatattctgg tgttgctccc
     1981 cagaatccag gaaagccatg ttgatcataa aaattaaatc ggtatttcca tttttcactt
     2041 aatgaatcat cgttcatata ttccttactc ataaatactc cataactatt gttttgatga
     2101 atcagtaggt gcaagcatta gcatactgaa agtggaaaaa taacaaatca aaaaaatcat
     2161 cgaaccattg cctgaacagg caaaatcttc ggctatcatt gtgatgatag agatgatata
     2221 tactgctaat gtaccaaaaa cataaatttt t
//
LOCUS       DAZPCS010000300         2230 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZPCS010000300 DAZPCS010000000
VERSION     DAZPCS010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.306"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            651..2159
                     /gene="ltrA"
                     /locus_tag="ACHUQ7_004089"
     CDS             651..2159
                     /gene="ltrA"
                     /locus_tag="ACHUQ7_004089"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFR1137239.1"
                     /translation="MQRKLATWAATDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALR
                     DRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEG
                     DLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVI
                     SPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCR
                     YADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRIIR
                     KRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYQF
                     VDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPKPGQSKTWVLFGKTNH
                     GKLSGEILYRLVGQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAMAFASI"
BASE COUNT          572 a          548 c          657 g          453 t
ORIGIN      
        1 ggcatggatg cttaaatgaa ccgcgagtct gtctggaata ttgaaccggt aactcacgat
       61 gagaaaccca acaatcccac cgggtgtgac ggtggagaac ctgagcggca gtgacctgcg
      121 gcatgcccgc agggtgatgt aacccgctga caacggggat tgaggcgaga tcactaagcc
      181 gagatgatcc tcaaggttaa gtactgaaag gctgaagaac atgaacccgt taatccgcct
      241 ctgtgggttg aaaacgtcac cacggcctac gtgatctgac aggccgtgca ggaggaactg
      301 gcagtgatac gtaagcactg ccggtcgaag gtgttttgac atgtatgcga aacaccgggg
      361 cagcagcgtc tatcacactt gcgttgctga cttctgccaa cttgcggcaa gcaaggataa
      421 agagtgcgac gggcagcctc ctcagtatgc ctgagtccag gcaggtaaac cggggaaggt
      481 cagcgacgga tgttaagggg gcatggctcc gatgacgcgc tggctggcgg agcttccgta
      541 gtagtccgcg atggggaaag cccattacat ggcgaaggga agcagtttga atgtgtttgc
      601 gacgtgaatt aactgaccta acgaggtgaa gacctttgat aatcagcgaa atgcaacgca
      661 agcttgccac atgggcagcc accgatccgt ccctacggat tcaacggctg ctgcgtctga
      721 taacacaacc agaatggctg gctgaagcgg cgcggatcac gctttcatca aagggggccc
      781 atacccccgg cgttgatggc gtgaacaaaa caatgctaca ggccagactg gctgttgagc
      841 tgcaaatcct cagggatgaa ttactctcag gccactacca gcccttgccc gccagacggg
      901 tttacatccc taaaagcaac ggcaaactgc gaccactggg tatccccgcg ttgcgggatc
      961 gtattgttca gcgggccatg ctgatggcga tggagccgat atgggagagt gattttcata
     1021 cgctctcata tggcttccgg cctgagcgca gtgtccacca cgcgatccgc acggtgaaat
     1081 tacagctcac agactgtggt gaaacccggg gacgctgggt gattgaaggc gacctgtcca
     1141 gttacttcga caccgtacat catcgactgc tgatgaaggc tgtacgccgc aggatcagtg
     1201 acgcacgttt catgactctg ctgtggaaaa ccatcaaggc gggacatatc gatgtcggtc
     1261 tctttcgggc ggccagtgaa ggtgtgccac agggcggtgt tatatcgccg ctattatcga
     1321 acatcatgct gaatgagttc gatcaatacc tgcatgagcg ctacctgagc gggaaagcca
     1381 gaaaagatcg gtggtactgg aataacagta tccaacgggg ccgaagtacg gcggtcagag
     1441 aaaactggca gtggaaaccc gcggtggcgt actgccgcta tgccgatgat tttgtcctca
     1501 tcgtcaaagg caccaaagca caggcggaag ccatcaggga ggagtgtcgg ggtgtgctcg
     1561 aaggcagtct gaaactcagg ctgaacatgg ataagactaa aatcacccat gttaatgacg
     1621 gctttatctt tctggggcac aggatcattc gcaaacgcag tcgttatggc gagatgcgag
     1681 tggtctcaac gatcccgcag gagaaagcca gaaacttcgc cgcatcgctg acagcactgt
     1741 tatcaggcaa ctacagtgaa agcaaagtcg atatggctga acaactcaac cgaaaactga
     1801 aaggctgggc catgttctat cagttcgttg attttaaggc caaagtcttc agttatatcg
     1861 accgtgtcgt gttctggaag ctggctcact ggctggcccg caaataccgt acaggtatcg
     1921 cttccctgat gaggtggtgg tgtaaatcac cgaaaccggg tcagagcaaa acgtgggttt
     1981 tatttggtaa aaccaatcac ggcaagctca gcggcgaaat actgtaccgg ttggtggggc
     2041 aaggcaagaa gctgttccgc tggcggctac ccgaaggtaa tccctatctg aggacggaga
     2101 ccagaaacac gtatacatcg cgctttacag aagtggcaat ggcgttcgcc agcatttaaa
     2161 tggagagccg gatgcgctga aaggtgcacg tccggttcgg ggaggagagg cagggaaata
     2221 gtccgactac
//
LOCUS       DAZPCS010000301         2228 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZPCS010000301 DAZPCS010000000
VERSION     DAZPCS010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.307"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..1224
                     /locus_tag="ACHUQ7_004090"
     CDS             <1..1224
                     /locus_tag="ACHUQ7_004090"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFR1137240.1"
                     /translation="FPYGIFQQRNVLASWLATGCGVALYLALTARTRSLTLICLLSLY
                     PLSTALVLTQSRVGLLGLFCVIVLAALADRPRWRGRPLSGLLRLIFVASLLCWCVGIS
                     NYAMPGETTADFTHSSSNDQRIRILKGAISLIQQRPLTGWGLGSFEATFPQALEDVGV
                     TNIESDTATHPHNELLFVTAEGGIVALFGFLLLSGVWLWPCICRLKMTNNNAHHQCEK
                     WFIALMGLPLVIHMMTEYPLYTSVPHLMLLLLLFRIGMPENVMNQVRIPKTVRLGILP
                     VIVLIMTAALVILVAGFDAQRELTRAEADMNEGFIPRLPEHSWKYLTQAERLERDQHL
                     LIANKPGFVRDTESMSLFTVWGNKWLAIHNDADVSAAMIFIAHRRGDYLTAEKIRKRA
                     ARVFVNDERFTRGGN"
     gene            1224..1958
                     /locus_tag="ACHUQ7_004091"
     CDS             1224..1958
                     /locus_tag="ACHUQ7_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR1137241.1"
                     /translation="MVNDLTYKKLESTNINCNILSPENTTWTNVTVIGLDLFAICGVC
                     SLLKRVGGVKLQVFTSLPTDMNVLTDSSIVIWVKMRHDGLPELAGHIVKVCRRYRLMR
                     QLVISDAIPEGMTNDIGPLSRVWLTHGSAKVDILLSSLRKVLNAHCLPGPLFIKRLGR
                     VQWRVLLLRAKGISTRKIAEICGIGLKTVSAHESAIRERLGTRNKSEYVWLVRNAQLI
                     QQAIPALGRDASRYERCNNMRTKQKV"
BASE COUNT          561 a          456 c          566 g          645 t
ORIGIN      
        1 tttccgtatg gcattttcca gcagcgaaat gttcttgcga gctggctggc aacaggttgc
       61 ggcgtagcgt tgtatctggc tttaacggcg cgtactcgtt ctctgacgtt gatatgtttg
      121 ttgtcgttat accctctgtc tacagcttta gtccttacac aaagccgtgt cggccttctt
      181 ggtttatttt gcgtcatcgt gctggccgct ctcgcggaca gaccccgctg gcgcggacgt
      241 ccccttagcg gtctgttgcg cttgatcttc gtcgcctcct tgctgtgctg gtgtgtcggt
      301 atctcaaact atgccatgcc aggggagaca acggctgact ttacgcattc atcatcgaat
      361 gaccagcgta tacgtattct gaaaggtgcc ataagtctta ttcaacagcg ccccttaacc
      421 ggttgggggt taggcagctt tgaagcgacc ttcccgcaag ccttagagga tgtgggcgtg
      481 acaaacatag agagtgatac ggctacgcat ccccataacg aactgctgtt cgtaacggct
      541 gaaggtggca tcgtcgcctt gtttggtttt ttgctgctat caggcgtttg gctgtggccg
      601 tgtatatgcc gcctgaaaat gacaaacaat aacgcgcacc atcaatgtga aaagtggttt
      661 attgccttaa tggggttgcc tttagtcatt catatgatga ccgaatatcc tctctatacg
      721 tctgttcccc atttgatgct gttgctgctc cttttccgca tcggaatgcc ggaaaacgtg
      781 atgaatcagg tacgtatacc taaaacagtc cggttgggta ttctgcctgt cattgtgctc
      841 attatgaccg ccgcgctggt catcctggta gcgggatttg atgcacagcg ggaactgacc
      901 agagctgaag ctgatatgaa cgaaggattt atccctcgcc ttccggaaca tagctggaaa
      961 tacctgacgc aggcagaacg tcttgagcgc gatcagcatc tgcttattgc gaataaaccg
     1021 gggttcgttc gcgatacgga aagcatgtct cttttcactg tgtggggaaa taaatggttg
     1081 gcaatccaca atgatgcaga cgtaagcgct gcaatgatat tcattgcgca caggcgcggt
     1141 gattatctga cagctgaaaa aataagaaaa cgcgcagctc gggtatttgt aaatgacgaa
     1201 cgttttactc gcgggggaaa ctaatggtta acgatctgac gtataagaaa ttagaaagca
     1261 caaacatcaa ctgtaatatt ctctcgccag aaaataccac atggacaaat gtcacggtga
     1321 ttggcctgga tctttttgcc atttgcggcg tgtgcagttt gctcaagcgt gttgggggag
     1381 tgaaacttca ggttttcact tccttaccca ctgatatgaa tgttctgact gacagtagca
     1441 tcgtgatatg ggtaaaaatg cgtcacgatg gtttgcctga attagccgga catattgtga
     1501 aggtttgtcg tcgataccgt ctgatgcggc aactcgttat tagcgatgct attcctgaag
     1561 gaatgacgaa tgatatcggg cctctttccc gagtatggct tactcatggg agtgcaaaag
     1621 tggatatttt attatcttca ttacggaagg tgttaaatgc tcattgtttg cctggtcctt
     1681 tgtttataaa acgcctggga agagtgcaat ggagggttct tttactccgc gcgaagggga
     1741 ttagcacacg taaaattgca gaaatctgtg gtattggcct caagaccgtt agcgcccatg
     1801 agtccgcaat tcgagaacga ttaggcacca ggaataaaag tgagtacgtc tggttagtac
     1861 gtaatgctca acttatacaa caggcgatac ccgcgttggg aagagatgcg tcaaggtatg
     1921 aaagatgtaa caatatgcga acaaagcaaa aagtataatt gatgcgtcat agataactct
     1981 ctttttcgat gacgaaatcg ctgagcatgc gtggtgaggt tagtaataga tgggtgctat
     2041 gttttgccac gggtttgtag ctgtgaaacc tggaaaaaaa taagaattaa aaatatttaa
     2101 agagagttaa taaggttctg gttgtaattg tcttggtcaa aaaaaagata ttgtcaatgt
     2161 tgttttttaa attttcaggt tttggttaaa tatttgcttg caaatatagg tgatttttta
     2221 ttgtaggc
//
LOCUS       DAZPCS010000302         2221 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZPCS010000302 DAZPCS010000000
VERSION     DAZPCS010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.308"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..373
                     /gene="tnpA"
                     /locus_tag="ACHUQ7_004092"
     CDS             <1..373
                     /gene="tnpA"
                     /locus_tag="ACHUQ7_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HFR1137242.1"
                     /translation="RPNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQY
                     RKGGLLPSGKNMPALLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLRL
                     KGKLTPALLQILIREIKGSSH"
     gene            373..720
                     /gene="tnpB"
                     /locus_tag="ACHUQ7_004093"
     CDS             373..720
                     /gene="tnpB"
                     /locus_tag="ACHUQ7_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR1137243.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLVSKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            740..1969
                     /locus_tag="ACHUQ7_004094"
                     /pseudo
     CDS             740..1969
                     /locus_tag="ACHUQ7_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            1973..2132
                     /locus_tag="ACHUQ7_004095"
                     /pseudo
     CDS             1973..2132
                     /locus_tag="ACHUQ7_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          538 a          593 c          619 g          471 t
ORIGIN      
        1 gcggcctaat tttccttatg aatttaaaat cgctttagtg gagcagtcac tgcagcccgg
       61 agcctgtgtg gcgcagatcg cccgtgaaaa cggaatcaac gataacctgc tcttcaactg
      121 gcgccatcaa taccggaaag gtggcctgct gccttccgga aaaaatatgc ctgcactgct
      181 tcccgtgacg ttaacgccgg agccggataa taaaatcccg gctccggcac aggaaccaga
      241 gcagataaat acaccgtccg acagtctgtg ctgtgagctg gttctgccgg ccggaactct
      301 caggcttaaa ggtaaactga cgccggcgtt attacagata cttatccgcg aaataaaagg
      361 gagcagccac tgatgatatc tttccctgca ggttcgcgta tctggctggt tgcaggtatc
      421 accgatatgc gaaatggctt taacggcctg gtctcaaaag ttcagaacgt cctgaaggat
      481 gacccgttct ccgggcatct gttcatcttc cgcggacgcc ggggtgacca gataaaagtg
      541 ttgtgggctg acagtgacgg actgtgcctc tttaccagac gcctggagcg gggccgcttc
      601 gtctggccgg tcacccgtga cggcaaggtg caccttactc cggcccagtt atccatgctt
      661 cttgagggca tcgactggaa acacccgaaa cgaacggaac gcgctggcat ccgcatataa
      721 cccgttgtaa agtgaggata tggacacctc acttgctcat gagaatgccc gcctgcgggc
      781 actgttgcag acgcaacagg acaccatccg ccagatggcg aaatacaacc gcctgctctc
      841 acagcgggtg gcggcttatg cttccgaaat caaccggctg aaggcgctgg ttgcgaaact
      901 gcagcgcatg cagttcggta aaagctcaga aaaacttcgc gcaaaaaccg aacggcagat
      961 actggaagca caggagcgaa tcagcgcact tcaggaagaa atggcggaaa cgctgggtga
     1021 gcaatatgac ccggtactgc catctcccct gcgccagtct tcagcccgta aaccgttacc
     1081 agcctcactt ccccgtgaaa cccgggtcat ccgtccggaa gaggaatgct gcccggcctg
     1141 tggtggcgaa ctcagttctc tgggatgtga tgtgtcagag cagctggagc ttatcagcag
     1201 cgcctttaag gttatcgaaa cacaacgtcc gaaactggcc tgttgccggt gtgaccatat
     1261 cgtgcaggca ccggtgcctt caaaacccat tgcacgcagt tatgccggag cggggcttct
     1321 ggcccatgtt gttgctggaa aatatgcaga ctatctgccg ttataccgcc agtcagaaat
     1381 ataccgtcgt cagggcgtgg agctgagccg tgccacactg gggcgctgga caggtgctgt
     1441 tgctgaactg ctggaaccgc tgtatgacat cctgcgccag tatgtgctga tgcccggtaa
     1501 agtccatgcc gatgatatcc ccgttccggt ccaggagccg ggcagcggta aaacaaggac
     1561 tgcccggctg tgggtctacg ttcgtgatga ccgtaacgcc ggttcggaaa tgcctccggc
     1621 ggtctggttc gcgtactcac cggaccggaa aggtatccat ccacagaatc atctggccgg
     1681 ttacagcgga gtgcttcagg ccgatgctta cggtggttac cgggcgttgt acgaatccgg
     1741 cagaataacg gaagccgcat gtatggctca tgtccggaga aaaatccacg atgtgcatgc
     1801 aagagtgcca accgacatca ccacggaagc cctgcagcgt atcggtgaac tgtatgccat
     1861 cgaggcagaa gtccggggat gttcagcaga acagcgtctg gcggcaagaa aagccagagc
     1921 cgcgccactg atgcagtcac tgtatgactg gatacagcaa cagatgaaaa ttcattcgct
     1981 gaaaatggaa tgtctccacg gagaacacta ttatccatcg ggaaatagtg cgggcaacag
     2041 tgtttagtta tatcgagtgc gattacaatc gctggcgacg gcaccgttgg tgtggtggtc
     2101 tcagtccgga acaatttgaa aaccagagcc tcacttacga ctgtgtccat attatgtggg
     2161 taggatcaat aaagacgttt tatatgtgtc tgcttgcagc cagaaaattt ataccctgca
     2221 g
//
LOCUS       DAZPCS010000303         2218 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZPCS010000303 DAZPCS010000000
VERSION     DAZPCS010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.309"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(100..525)
                     /gene="arsC"
                     /locus_tag="ACHUQ7_004096"
     CDS             complement(100..525)
                     /gene="arsC"
                     /locus_tag="ACHUQ7_004096"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFR1137244.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(538..1827)
                     /gene="arsB"
                     /locus_tag="ACHUQ7_004097"
     CDS             complement(538..1827)
                     /gene="arsB"
                     /locus_tag="ACHUQ7_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HFR1137245.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(1881..2216)
                     /gene="arsR"
                     /locus_tag="ACHUQ7_004098"
     CDS             complement(1881..2216)
                     /gene="arsR"
                     /locus_tag="ACHUQ7_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HFR1137246.1"
                     /translation="MQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQPKISRH
                     LALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRNLARQNC
                     SGDSKNICS"
BASE COUNT          589 a          611 c          555 g          463 t
ORIGIN      
        1 gaatttgcac ggttttgtag gtcggataag acgcgttagc gtcgcatccg acataaacaa
       61 agcgcacttt accaactatc ggcagtggat atcgccgctt tatttcaggc gcttacccgc
      121 ttcatcaacc actttctcgc catcttcctt ggagaatgcg cctttttgcg catctggcag
      181 aatttccagc accacttctg aagggcggca caggcgagtt cccagcggcg tcaccacaat
      241 cgggcgatta atcagaatcg ggtgctgaag cataaagtcg attaaccgat cgtcagtaaa
      301 tttatcttcc gcaaggccca gctcctcata cggttcgacg tttttacgca gcagcgcgcg
      361 tacggaaatc cccatatcgg caatgagttt gaccagttca tcgcgcgttg gcggagtttc
      421 cagataatgg ataatggtcg gttctgtgcc gctgttgcgg atcatctcca gcgtattacg
      481 cgacgtgccg caggccgggt tgtgataaat ggtaatgttg ctcatatcag tatctcatta
      541 caaagtgaaa gagagacgta gcgccagcgc agccagcgtc acaaacagca caggcagggt
      601 catgataatc cctgtacgga agtaatatcc ccagctgata gtcatattct tctgcgaaag
      661 tacgtgcagc cagagcagcg ttgccaggct accaatcggg gtaattttcg gccccaaatc
      721 gcagccaatc acgttggcat aaaccatcgc ttctttgata acgccagatg ccgtgctgcc
      781 atcaatggac aacgcgccaa ccagtaccgt cggcatattg ttcataatag aagagaggaa
      841 ggcggtgagg aatccggtgc cgagcgtcgc ggcccacagg ccgttatccg ccagcacgtt
      901 gagtacgcca gaaagatatt ccgttaatcc ggcattgcgc aggccataaa ccaccagata
      961 catgccgagc gagaagatga caatctgcca gggggcaccg cgcaggactt tacccgtatt
     1021 aatcgcatga ccgcgtttag cgacgacaaa taatatcagt gcgcccacag ctgcaatggc
     1081 gctcaccgga atgccgagcg gttccaggac gaaaaatccc accagcagaa gcagtaaaac
     1141 aacccagcca gttttgaacg tagcaggatc tttgatcgct tctgcgggag atttcagcag
     1201 cgccatatcg tagttctgcg gaatatcttt gcgaaaatag agatgtagca tcaccagcgt
     1261 ggcaacaatc gcggcgatat ccaccggcac catcaccgag gcgtattcgc gaaagccgag
     1321 gccaaagaaa tcagcggaaa cgatattcac caggttggag acaataagcg gcaggctggc
     1381 ggtatcggca atgaatccgg ccgccatcac gaacgccagc gtcgtgcctt tactgaatcc
     1441 taaagccagc agcatggcga tgacaatcgg cgtcaaaata agcgccgcgc catcattggc
     1501 aaacagggcc gcaacggcgg caccgagcag gacaatccag gtaaacagca agcgaccgcg
     1561 tccattgccc cagcgtgaaa cgtgcagcgc tgcccattca aaaaagccgg actcatccag
     1621 cagcaggctg atgataatga cggcaataaa cgccgccgtc gcgttccaga cgatattcca
     1681 caccaccgga atatcacccg gatgaaccac gcccgtaacc aacgccagta ccgcgccgag
     1741 cgttgcactc cagccgatgc ctaaaccttt cggctgccag ataaccaata cgatggtcag
     1801 gacaaagata gcgcctgcca gtaacataat gcctcccgga taaaacacat gtgcaaatcc
     1861 atatgtgttt agctaaattt ttaactgcaa atgttcttac tgtctccgga acagttttgt
     1921 ctggccaggt tgcggacaat tgcctgaatc ttttcctgtt cacatcgcca ggcttcatca
     1981 ataattttcg ccgcccatgc tggaatatgc ggcgataagc ggtaatgaac ccacttacct
     2041 tgcttgcggt ccagcaatag cccgctttca cgcagcaatg ccagatggcg ggatatcttg
     2101 ggctgcgact gatcgagggc agtgcagaga tcgcagacgc ataactctcc cagttcgcta
     2161 agcagtaaaa cgatgcccag acgggtttca tcagcaagaa ttttgaacaa ttggatgg
//
LOCUS       DAZPCS010000304         2215 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZPCS010000304 DAZPCS010000000
VERSION     DAZPCS010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.310"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            137..1219
                     /locus_tag="ACHUQ7_004099"
     CDS             137..1219
                     /locus_tag="ACHUQ7_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HFR1137247.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
     gene            complement(1708..2215)
                     /locus_tag="ACHUQ7_004100"
                     /pseudo
     CDS             complement(1708..2215)
                     /locus_tag="ACHUQ7_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT          652 a          453 c          447 g          663 t
ORIGIN      
        1 gataaaaata gggatctact tcacaattta aatgagaaac taaaacttac atcttgaaat
       61 aatctcattg attggatgaa tatttgtagt gcagtgacat catttttaaa taatagttca
      121 aataaaaggg ctcacgatga aaaaattaac agtggcaatt tctgctgtag ctgcatcagt
      181 actgatggcg atgtctgctc aggcagcaga gatttacaat aaagacagta acaagctgga
      241 tctgtacgga aaagttaatg ctaagcacta cttctcttct aacgatgcag atgatggcga
      301 cacaacttat gttcgtctgg gcttcaaagg cgaaactcaa atcaacgatc aactgactgg
      361 tttcggtcag tgggaatatg aattcaaagg caaccgtgct gaatctcaag gttcttccaa
      421 agataaaacc cgtcttgcct tcgctggcct gaaattcggt gactacggct ccatcgatta
      481 tggccgtaac tacggtgtag catacgatat cggtgcatgg accgacgttc tgccagaatt
      541 cggtggcgat acctggaccc aaacagatgt gttcatgact ggtcgcacca caggtgttgc
      601 aacctatcgt aacaacgact tctttggtct ggttgatggt ctgaactttg ctgctcagta
      661 tcagggtaaa aatgaccgca ctgacgtaac tgaagctaac ggtgatggtt tcggtttctc
      721 cactacttat gagtatgaag gattcggtgt aggtgcaacc tatgctaaat ctgaccgcac
      781 taatgatcag gttatctacg gtaacaacag cctgaatgca tctggtcaaa atgctgaagt
      841 atgggcagct ggtctgaaat atgatgcgaa caacatctat ctggctacca cctattctga
      901 aacccagaac atgactgttt ttggtaataa ccatattgcc aacaaagcac aaaacttcga
      961 agtagttgca caatatcagt tcgacttcgg tctgcgtccg tccgttgctt acctgcaatc
     1021 taaaggaaaa gacttgggtg cgtggggtga tcaggacctg attgaatata ttgatgtagg
     1081 tgcaacctat tacttcaaca aaaatatgtc cacttttgtt gattacaaaa tcaacctgat
     1141 tgataagagc gatttcacga aagcatctgg cgttgctacc gatgatatcg ttgctgtagg
     1201 attggtttac cagttctaat ctgattactc aaagatatgt tgtgggaggc tttgcctccc
     1261 caacatataa gtggcgctct caagccactt tccttagaag cactatcttg cttctgacta
     1321 tataaattac cctttatttt ctgggacgtt tcaacgcccc atttttaata atactcagtg
     1381 aacaattaac gtatcaatta gatttaataa caacgatatc cgtacctaac ctgatatcta
     1441 atgtcataag catcccttta attatcccct caacctcttg taaatttccc actcttgagc
     1501 tccacatagc ccaaacgccg gatgcaaaaa aaatcatacg tgcatctctg aaattgtgat
     1561 ggaggaagtg agtatcaatt atatgtgctt ataaacgttc ccgattggca cagaagtcac
     1621 cggattgttc aggctccaat aggttgagta tgtcgtattt attttccaaa ataaaaatcg
     1681 atgctgttgc tccttaataa aatactttta ctctctggac gcttttctta gctctcttgg
     1741 tgttatatta aaacgattat ggaacctttc agtaaaacga gaaggacact tgtaaccatt
     1801 ttccctggca atctcgctga tgggttttac tgtcgtttgt atagcaggac aacgcattat
     1861 ttaacctcac gtcgtccaat atactttgaa aacttattcc ctcgcttgcc agacggcgat
     1921 gcaatgtgga aacagaaatg tagagatatc tcgctacctt gtttgctgtc cattttgttc
     1981 taggttcgga tagcattagg ttataacaac gacttattaa tgattgttta ctatctgata
     2041 aaagtaaaca attaacatga tgtgctccta atgaaagtag aacgcccatt gctaagtgtt
     2101 cctgaatttg cgttgagaag cctcgggaaa tagatgtttt tagttgctcc caacaatata
     2161 ttaactcggg gttctgaggt aagcagaaac ctgtcgtgtt acgaatttga tcagt
//
LOCUS       DAZPCS010000305         2188 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZPCS010000305 DAZPCS010000000
VERSION     DAZPCS010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.311"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(221..385)
                     /locus_tag="ACHUQ7_004101"
     CDS             complement(221..385)
                     /locus_tag="ACHUQ7_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1137248.1"
                     /translation="MTLTTTALNGSSSRRFEGCVWTPPLISHTALHPTSRHWMHSWHT
                     YAGNAEIPGL"
     gene            1287..1571
                     /locus_tag="ACHUQ7_004102"
     CDS             1287..1571
                     /locus_tag="ACHUQ7_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFR1137249.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            1559..2044
                     /locus_tag="ACHUQ7_004103"
     CDS             1559..2044
                     /locus_tag="ACHUQ7_004103"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR1137250.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSSEAVLDDWLKQKELKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
                     Q"
BASE COUNT          565 a          462 c          620 g          541 t
ORIGIN      
        1 ttcaaaatga tggacattat ttttgtggaa ccggaggtaa cagaccagac ggtttaaatg
       61 agccggttac tttaaacggg tgatggacag cacctcaaaa gttcagatgg attatatttg
      121 tctgcaatac cccgggtatt ttcgatttgc gaaatggatg gaacgacttg ctcaggggat
      181 agctgatgga gttactgaag taccgaagga ccactaaaaa tcacaggccg ggaatttcgg
      241 cgtttccggc gtaggtgtgc cacgaatgca tccagtggcg tgatgttgga tgcaatgctg
      301 tatgagagat gaggggcggc gtccagacgc agccttcaaa ccgccttgag ctgctgccgt
      361 taagagcggt ggtggtgagc gtcaaggtta aaaggcgcag aacatgtgtc aggtaggtgt
      421 tgcggagatg aacgaaagtg aactgtcgat gaagtgtcga aaggaacctc taggcgatgt
      481 cgaaaatgtg ctatctacgc agcgcatgac gagtccagca gatatctggt actggctggg
      541 cggcatccgg catataggca gcatgaacgc aacacaggct ctcgtacgga acgtgggaac
      601 ctgtcgtctc gatgctaagg gagcggcgcg gagcagaggc actgcaagcg cctgagtacc
      661 gatgcgagac acaggggcag atggcttcgt agtagtgagg aagtgcctgt aatgggcatg
      721 gagcgaaggg ggtcagtcgt tggggctggt ttatcggtca accggatagc cgggaggagc
      781 ccatgagtca gccaaagccg ttcgaggtct ctaagtatgc ggtttggaaa gcataccagc
      841 gggtcaaagc taatcgaggt gcggcaggtg tcgatgggca gtcagtcgag gcgtttgagg
      901 ttaccaatcg aagctatacc cggtaatggc gcaggttgac ctgcacttgg ccaagtggat
      961 tgcccgcaaa cacaagcgag cgcgaggtag tctggtgcgt gcattcgagt ggttactgcg
     1021 aattcgtcac gactgcccga cgctgtttgc acactggtgc ttagcttacg atacttgaat
     1081 ggttcaacga caagagccgt atgacgcgag agtgtcacgt acggctccgt gggagcgtag
     1141 gggggcggtg cccctgcgcc acccgatatc ccctttgagt gcggttacgt ccacagaggt
     1201 aaatgccgct gacgcggcat ctgtaatctg ttactttgtg tatatcattt gctatatatt
     1261 gtcaatatgc tattgttgta aagcaaatga tatacggagg ctttatgaaa tcaggtgttc
     1321 agctcaatct cagagcaaga gaatcacagc ggatactcat tgatgctgct gcagaaattc
     1381 ttcataagtc gcgtacagat ttcattctgg aaatggcctg taaagctgct gaagatgtta
     1441 ttctcgaccg gcgggtgttt aattttaatg acaggcaata tgaagagttc attgaaatgc
     1501 ttgatgctcc ggttgcagat gatcctgcca tcgaaaaatt gctggcaagg aaacctcaat
     1561 gggatgtgta actgcaccag aacctttatc ctcatttcat caggtcgctg aatttgtcag
     1621 cagtgaagct gtacttgatg actggttaaa gcagaaggag ctaaaaaatc aggcaatcgg
     1681 agccacccgg acttttgttg tatgcagaaa aggtacgcaa caaatagtcg gtttttactc
     1741 actggccact ggtagtgtca atcatacaga agctacaggt aaccttcggc gcaatatgcc
     1801 agaccccatt cctgtaatta tacttgcacg cctggctgta gatgtgtcat ttcgaggaaa
     1861 agggcttggc gcggatttac ttcatgatgc agttcgtcgt tgttacaggg ttgccgaaaa
     1921 tatcggtgtg cgtgcaatta tggttcatgc gcttacggaa aacgcaaaac agttttatat
     1981 tcatcatggt tttaagccat caaaaactca ggtgcaaaca ttgtttctta aactccctca
     2041 atagggagta atgccgatca gttaaggatc agttgaccga tccagtggct gtgtaagaat
     2101 ccggaaacgc tcactcgttt ccggattttt ctgtgcacag taaaagacca cctcctgagg
     2161 aggtggcttt tcagaatgcc ccctataa
//
LOCUS       DAZPCS010000306         2185 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZPCS010000306 DAZPCS010000000
VERSION     DAZPCS010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.312"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..205
                     /locus_tag="ACHUQ7_004104"
     CDS             <1..205
                     /locus_tag="ACHUQ7_004104"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1137251.1"
                     /translation="ARAGVPGSDEETRADFENRRRNSVARNARNILEAIRGEILSTVE
                     NVVDVYVTHNPKKTEQKAGVSIH"
     gene            295..696
                     /locus_tag="ACHUQ7_004105"
     CDS             295..696
                     /locus_tag="ACHUQ7_004105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1137252.1"
                     /translation="MNGNTTFTVADKEYDPPYPEYVISWQTLKPVSLHVSVTLKKSDY
                     LPSDITRQVQQSVLDAFNGTDGGLRARVASVVSAGRYYAGIYKTDPEHIDILGLTVSR
                     DGSSWTTAVTFGIDEIPVLDVSDISVKLQEA"
     gene            698..1303
                     /locus_tag="ACHUQ7_004106"
     CDS             698..1303
                     /locus_tag="ACHUQ7_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HFR1137253.1"
                     /translation="MQNVAATVLAQYAASPRLNALINSFNAALSPDSFISDFYGLIWN
                     IDTAEKYGLDVWGKIVGVSRWLTVKDDFNYLGFSESRMDTPVMDDPCPFNQAPFYNGK
                     SDTRTVDLSDAVYRRLILMKAMSNITDCSVPDINLMLRFMFGKKRRAYVLNNGGLRMS
                     YVFESALSLAELAIIQSSGALPSPPGVYVSVVLKESRNEGQ"
     gene            1290..>2185
                     /locus_tag="ACHUQ7_004107"
     CDS             1290..>2185
                     /locus_tag="ACHUQ7_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1137254.1"
                     /translation="MKASDKPRQLAVPFASTGDKNRIPDKATQQTRESGNAAYDSGFP
                     PVTMTAVSAGGIPPHGKDFNGLMYDITAAIRFAQAGGLYTYNAGFAGAIGGYAKGAIL
                     AGVATTAVWLNTTDDNLTDPEGSDSAGWVNLLEDPKRIFLRQKNNLSDLQNKGTARDN
                     LQVYSKEQSDQRYVHREGDKITGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSE
                     GQGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGD
                     NDTGFKQNGDGILDVYANSQRV"
BASE COUNT          531 a          508 c          610 g          536 t
ORIGIN      
        1 cgcccgtgcc ggtgtgccgg gcagcgatga ggaaacccgt gcggactttg aaaaccgccg
       61 acgtaattca gttgcccgta atgcccggaa tattctggaa gccatccggg gtgaaatact
      121 ctccacggta gaaaacgtgg tggatgttta cgtcacccat aatccgaaaa aaacagaaca
      181 aaaagccgga gtcagtatcc attaacaccc ggttcgcttt atgttggcgt gtacggcggc
      241 agcccggcag atatcgcggc ggccatctgg cgtaaggctc cgccgggtat tgatatgaat
      301 ggcaacacaa cgttcactgt tgcagataag gagtacgatc cgccgtatcc tgaatacgtg
      361 atcagctggc agacactcaa acccgtcagt ctgcatgtca gtgtgacgct gaaaaaaagt
      421 gactacctgc cttcagatat tacccgacag gtacagcaat ccgtgctgga cgcatttaat
      481 ggtacagatg gtggtctgcg ggcaagagtt gcctctgttg tctctgccgg gcgctactat
      541 gccggcattt acaaaaccga tccggaacat attgatattc tgggccttac tgtgagccgt
      601 gatggttcgt catggacaac ggctgtcact ttcgggatag atgagattcc ggttctggat
      661 gtgtcggata tcagtgtgaa actccaggag gcataacgtg cagaatgtgg ctgccactgt
      721 gcttgcgcag tatgctgcca gcccccgact caatgccctc attaacagct ttaacgcagc
      781 gctttccccc gacagtttta tcagtgattt ttatggtctt atctggaaca tcgataccgc
      841 agaaaagtat ggtcttgatg tctggggaaa gattgtgggt gtcagtcgct ggctgacggt
      901 aaaggacgat tttaattacc tgggcttcag cgagtccagg atggacaccc cggtaatgga
      961 tgatccctgt ccgtttaatc aggcaccgtt ttacaacgga aaatcggata cccggactgt
     1021 tgacctgtct gatgctgtat accggcggct gatactgatg aaagccatgt cgaacattac
     1081 tgactgttcc gttccggata ttaacctgat gctgagattt atgttcggaa aaaaacgccg
     1141 ggcttatgtt ctgaataatg gcgggctgag gatgagttac gtctttgagt ccgcgctctc
     1201 gttggcagaa ctggcgatta tccagtcgtc gggtgcactg ccatccccgc cgggtgttta
     1261 tgtttcagta gttttaaagg agtcccgtaa tgaaggccag tgataaaccc cgccagctgg
     1321 cggtcccctt tgcgagtacc ggagataaaa accgtatccc ggacaaggcg acacagcaga
     1381 ccagagagag cggtaatgct gcgtatgatt caggttttcc tccggtgacc atgacagcgg
     1441 tctcagcggg aggtataccg ccacacggca aggattttaa cggtctgatg tacgatatta
     1501 ccgcagcaat acggttcgcc caggctggcg gtttgtacac gtataatgcc ggttttgcgg
     1561 gggccattgg tggatatgca aaaggagcca ttctcgccgg agtcgcaaca acagcggtct
     1621 ggctgaatac cacggacgat aacctgaccg atcctgaagg ctccgacagt gcgggctggg
     1681 taaatcttct tgaggatccg aaaaggatat tcctgcggca gaagaacaat ctgtcagacc
     1741 ttcagaataa agggacggca cgggataatc ttcaggttta cagtaaagag cagtcagatc
     1801 aacgctatgt tcatcgggaa ggcgataaaa taaccggaga gctgaaaatc cgtggtgtta
     1861 atgcgctgag gattttcaac gaagcttttg gcctgatttt tcgtcgttcg gaagagtgcc
     1921 tgcaccttat tcccaccagt gaaggtcagg gggaaaatgg cgatattggt cccctgcgcc
     1981 cgttcaccat taatttgcgg acgggtgaaa tatccatgtc gcataaagtg tctgttggcg
     2041 gtggttcgca ggtcaatggt gcgctgggta tcggcgttca gaacgccctg ggggggaatt
     2101 caattgttct tggtgataat gacaccggat ttaaacagaa tggagacggt attctggatg
     2161 tttatgctaa tagtcagcgt gtatt
//
LOCUS       DAZPCS010000307         2165 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZPCS010000307 DAZPCS010000000
VERSION     DAZPCS010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.313"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            48..122
                     /gene="tnaC"
                     /locus_tag="ACHUQ7_004108"
     CDS             48..122
                     /gene="tnaC"
                     /locus_tag="ACHUQ7_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006569 - tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HFR1137255.1"
                     /translation="MNILHVCVTSKWFNIDNKIVDHRP"
     gene            343..1758
                     /gene="tnaA"
                     /locus_tag="ACHUQ7_004109"
     CDS             343..1758
                     /gene="tnaA"
                     /locus_tag="ACHUQ7_004109"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HFR1137256.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1849..>2165
                     /locus_tag="ACHUQ7_004110"
     CDS             1849..>2165
                     /locus_tag="ACHUQ7_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFR1137257.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYIS"
BASE COUNT          587 a          483 c          511 g          584 t
ORIGIN      
        1 tctgagtgta ataatgtagc ggcgtgtctt tcgaggataa gtgcattatg aatatcttac
       61 atgtatgtgt gacctcaaaa tggttcaata tcgacaataa gattgtcgat caccgccctt
      121 aatttaccct tctgtagcca tcaccagagc caaaccgatt agattcaatg tgatcgatta
      181 gtttgctata tcttaatttt gccttttgca aaggccatct ctcgtttatt tacttatttt
      241 agtaaatgat ggtgcttgta tatatacatg gtgaattaat ggatattgca gatgtaatat
      301 tcacagggat cactgtaatt aaaataaatg aaggattatg taatggaaaa ctttaaacat
      361 ctccctgaac cgttccgcat tcgtgttatt gagccagtaa aacgtactac tcgcgcttat
      421 cgtgaagaag caattattaa atccggtatg aacccgttcc tgctggatag cgaagatgtg
      481 tttatcgatt tactgaccga cagcggcacc ggggcggtaa cccaaagtat gcaggcagcg
      541 atgatgcgcg gcgacgaagc ctacagcggc agccgcagct actatgcgtt agccgagtca
      601 gtgaaaaata tctttggtta tcaatatact attccgactc accagggccg tggcgcagag
      661 caaatctata ttccggtact gattaaaaaa cgcgagcagg aaaaaggcct ggatcgcagc
      721 aaaatggtgg cattctctaa ctatttcttt gataccacgc agggccatag ccagattaac
      781 ggctgtaccg tgcgtaacgt ctatatcaaa gaagccttcg atacgggcgt gcgttacgac
      841 tttaaaggca actttgacct cgaaggatta gaacgcggta ttgaagaagt tggcccgaat
      901 aacgtgccgt atatcgttgc aaccatcacc agtaactctg caggtggtca gccggtttca
      961 ctggcaaact taaaagcgat gtacagcatc gcgaagaaat acgatattcc ggtggtaatg
     1021 gactccgcac gctttgctga aaacgcctat ttcatcaagc agcgtgaagc agaatacaaa
     1081 gactggacca tcgagcagat cacccgcgaa acctacaaat atgccgatat gctggcgatg
     1141 tccgccaaga aagatgcgat ggtgccgatg ggcggcttgc tgtgcatgaa agacgacagc
     1201 ttctttgatg tgtacaccga gtgcagaacc ctttgcgtgg tgcaggaagg cttcccgaca
     1261 tatggcggct tggaaggcgg cgcgatggag cgtctggcgg taggtctgta tgacggcatg
     1321 aatctcgact ggctggctta tcgtatcgcg caggtacagt atctggtcga tggtctggaa
     1381 gagattggcg ttgtctgcca gcaggcgggc ggtcacgcgg cattcgttga tgccggtaaa
     1441 ctgctgccgc atatcccggc agaccagttc ccggcacagg cgctggcgtg cgagctgtat
     1501 aaagtcgccg gtatccgtgc ggtagaaatt ggctctttcc tgttaggccg cgatccgaaa
     1561 accggtaaac aactgccatg cccggctgaa ctgctgcgtt taaccattcc gcgcgcaaca
     1621 tatactcaaa cacatatgga cttcattatt gaagccttta aacatgtaaa agagaacgcg
     1681 gcgaatatta aaggattaac ctttacctac gaaccaaaag tattgcgtca cttcaccgca
     1741 aaactgaaag aagtttaatt aatactacag agtggctata aggatgttag ccactctctt
     1801 accctacatc ctcaataaca aaaatagcct tcctctaaag gtggcatcat gactgatcaa
     1861 gctgaaaaaa agcactctgc attttggggt gttatggtta tagcaggtac agtaattggt
     1921 ggaggtatgt ttgctttacc tgttgatctt gccggtgcct ggtttttctg gggtgccttt
     1981 atccttatca ttgcctggtt ttcaatgctt cattccgggt tattgttatt agaagcaaat
     2041 ttaaattatc ctgtcggctc cagttttaac accatcacca aagatttaat aggtaacacc
     2101 tggaacatta tcagcggtat taccgttgcc ttcgttctct atatcctcac ttatgcctat
     2161 atctc
//
LOCUS       DAZPCS010000308         2151 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZPCS010000308 DAZPCS010000000
VERSION     DAZPCS010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.314"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            1..958
                     /gene="espL1"
                     /locus_tag="ACHUQ7_004111"
                     /pseudo
     CDS             1..958
                     /gene="espL1"
                     /locus_tag="ACHUQ7_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            1130..1237
                     /gene="yniD"
                     /locus_tag="ACHUQ7_004112"
     CDS             1130..1237
                     /gene="yniD"
                     /locus_tag="ACHUQ7_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFR1137258.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1290..2048)
                     /locus_tag="ACHUQ7_004113"
     CDS             complement(1290..2048)
                     /locus_tag="ACHUQ7_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFR1137259.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
BASE COUNT          647 a          478 c          451 g          575 t
ORIGIN      
        1 gatgctcaga cctgggctga taggtatgtt cgcaactgtg caattgctat tctgccccta
       61 ttacctgtgg gagttctagg ggctattttc gcgggtattg catcacgaat gccatttgcc
      121 cctatacatc catcggcaat gttgttaata atggctacag gccagactca acagcttatt
      181 acattattca aacagttgcc catactccct gaaaaagaaa tcattgaaat aataactgcg
      241 cagaatagcg ttggtacacc tgctttattt ttggctatga tgaacggaca tactgacaac
      301 gtgaaaatat ttatgcaaga aattcagtca ctggtagata atcacatcat tcatgaagat
      361 aatctggtta aattactgca aactaaaagt gctaacgaaa cacctggact ttatatctcc
      421 atgttgtatg gattcgatga aataatcgat atctttctga atacattagc cactccaata
      481 gcactaagag cttttaaaca aaaaactggt gatgagtatt ttagccatga aaatacatga
      541 tggtgagcca ggattatacg ccgcaatgga aaataatcac cctttgtgtg tcacacggtt
      601 cctctctaaa attaatggca tcgcctttaa atacaagttg agcaaagcta acatcatgga
      661 tttattaaaa ggagctacag cacagggaac ccctgcatta tacatcgcca tgagcaaggg
      721 taatgaagac gtcgtgttat cttatatatc gacgctgggt gcttttgcaa aaaaacattc
      781 ttttagtcaa catcagttat ttacactatt ggccgctaaa aatcatgaca acatgtcagc
      841 tgttcatata gccattcatc ataagcatta taaaactgta gaaacatatt atgctgctat
      901 aaatgtcatc agccaaagcc tgagttttag tgctgatgaa ataaagacgt atttataacc
      961 agctatttac gatatatatc gatacataat tttatttttt tcataaaacg cagaaacagt
     1021 gaccatgtgc acaacgccac ccgacggctg taataacaaa aaatcccttg atgcctgtcc
     1081 cttttgttac actccgttat cacgcacaag agatatgcag gacactggta tgccgactaa
     1141 acgctttgat aaaaaacact ggaagatggt ggtggtgcta ctggcaatct gtggcgctat
     1201 gttgttgcta cgttgggcag caatgatttg gggctgagca gtgacaaacg aagacagcca
     1261 gaccgcataa cacggtccgg cagataattt tacatgctgt agcccagcga taaggttgtc
     1321 cgacggtcgg tatgttccgg tgcagactct ggaggttcag agttccatgt gacgttgtaa
     1381 gccaccttca gtccaaaatg ttcattaata gcaacattta atgcgctttc ggagttcagc
     1441 gttgtgtctt ccgcgccaaa gaccgaaaca ccctgcgtaa atttagcgtt gtcagtcaac
     1501 tgccatgcat aagccccgga ggcatagccc agcggctggg tttcactggc attgtcggta
     1561 tatttgtcgt aacgcacacc tggaccgaat tcaaagcgga aactgtgtac cgggccattc
     1621 aaaaactgac gaccataacc cgcggtcaac acatcgcgct ctcgatagcc gttataacgg
     1681 tctgtcagcc agcttgcctg accaaataaa tagtcataat cagttaaatt aaagcggcta
     1741 cgcccgcccg ccgcatattt ttctgaagaa cgctcatcat tagaagaagt attactggcg
     1801 ttcccccaca gcgaccaggc agtggtttgt ccataccagg tcatggtggt gtcagccgta
     1861 agtgaggagc ttttcgtatt gcctgattgt gcaagatatc ctgcgttcag attaccttcg
     1921 aaaggttttt tagcgctggc aggatcgtcc atgacagtaa aaacggaatc atcggcagct
     1981 gcattcagtg acgcaaacat gccccccgcc aacataacga tggcaggaac tgtcttcaaa
     2041 agcttcattt atcaagagtc cgtacaacaa aaaaagagac catcgcggtc ccggaaactt
     2101 tcttaaggat caaagattag cgtccctgga aaggtaacga attataaaaa g
//
LOCUS       DAZPCS010000309         2148 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZPCS010000309 DAZPCS010000000
VERSION     DAZPCS010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.315"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..278
                     /locus_tag="ACHUQ7_004114"
     CDS             <1..278
                     /locus_tag="ACHUQ7_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1137260.1"
                     /translation="FVSFVNREGKICHTAYVKSSDNSMTYYHANGSSIDKYITDMCGL
                     ICMRHIESTGIIFYMLDEKVLSAIAEFMNEKGWRAAFCSAKNLYKCV"
     gene            complement(387..645)
                     /locus_tag="ACHUQ7_004115"
                     /pseudo
     CDS             complement(387..645)
                     /locus_tag="ACHUQ7_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            608..1185
                     /locus_tag="ACHUQ7_004116"
                     /pseudo
     CDS             608..1185
                     /locus_tag="ACHUQ7_004116"
                     /inference="COORDINATES: protein motif:HMM:NF013753.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1218..1619
                     /locus_tag="ACHUQ7_004117"
                     /pseudo
     CDS             1218..1619
                     /locus_tag="ACHUQ7_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031967.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          620 a          431 c          531 g          566 t
ORIGIN      
        1 gttttgtcag ttttgttaat cgtgaaggaa aaatatgtca tactgcatat gtaaaatcat
       61 cagataatag catgacatat taccatgcaa atggtagcag cattgacaaa tatattacgg
      121 atatgtgcgg ccttatttgt atgaggcata ttgagtctac tggcattatt ttttatatgc
      181 ttgatgaaaa agttttatct gccattgctg aatttatgaa tgaaaaagga tggcgggctg
      241 cgttttgttc agcaaagaac ttatataaat gtgtttaatt ttagaaaaac tatttggtaa
      301 aatccaacaa gagtcgagag gttcgcgttg cttcagcagg gcctcagcct gttcaaccag
      361 actcagttgc aggtcgtgac gcagatttag tggtggtggt tgattgtcag ataccagccg
      421 atgatcttgt agtgcatatc atcagacttt gagaagcata tggttttgcg ggtaagccgc
      481 ttaagatgtg tccgcaggtt cagattatgt cgctctatcc gctgagtgca tttcttgctt
      541 ataacatgac tggttgagga caataaagtt cggtataccg gctcaggcat cggtcatgta
      601 gaaggcaatg ctaaactggc tcagcaaact aagagcagcg cgcatccact tacccaatgc
      661 ggttgagaaa atagccttcg atcgctttca tgtggcgaaa caaccgggtg aggtggtgga
      721 taaaacccgc cagaatgaac atccgcatct tcctgttgaa agtcgtcgtc aggcaaaggg
      781 aactcgcttc ctgtggcagc acagcgataa atggatgacc gaatccaggc aggaaaagct
      841 gatatggctg cgtgcgcaga tgaagctgac gagcctgtgc tgggcgctga aagaactggc
      901 aaaggatatc tggagcagac catggagcga ggaaagacgc aacgactggc agagatggct
      961 gcggccaaca gtgacgtccc catgatgaag aatgtggcta aaacgatagg gaaaaggttg
     1021 tacggtatcc tgaacgccat gcgccatggc gtatcaaacg gaaatgcgga agcactgaac
     1081 agcaagatca ggctgctgag aataaaagcc aagggctacc gcaaccggga gcgctttaag
     1141 ctgggagtga tgttccacta tgggaaactg aatatagcgt tctaagcctt cccaccatga
     1201 tcggggaagc cccagatatg aacaggcaca ttcagagtta ctttaaagag cttactgaac
     1261 gtctgaggtg ttagagccat gactgccgca gcattgctgg cggaaatgcc tgaatcgagc
     1321 agcctctcga gacgtgaaat cagcgcactt gtcggtgttg ctcaggtaaa ccgggactcg
     1381 ggaactctgc gcggtcgacg aaccattttc ggtgattgtg ctggggaaga acagctctgt
     1441 acatggcggc gcttgaggcc cccccccggt ttaatctggt gataaaggcg ttttacatgc
     1501 gcctacttgc agccggaaac gccaaaaaag tggcactggt tgcctgtatg cgtaagttgc
     1561 tgaccattat gaacgcaatg ctcagaaaaa acgaagaatg gaatgaatcg tatttatgaa
     1621 aactggattg tgggccataa atattccaaa aagatagccc gctccgttta ttaccggaac
     1681 gggcgagagc atgacattgt ctgttattat aatgagactg ctgattctac ttatttttaa
     1741 tttcaagaaa agcgttttct acaaagaatt atgagattta atgttttgtt tagacaatat
     1801 tatcattctg ttcaataatg aaagtcttca tcaacaacaa gtaaaaacaa cgtccctctc
     1861 atcgaaccgg acttgcagat ttcccgcatc cggctctctt gcaacacatc agaccttcac
     1921 gttcgggcgg ttatggggaa ggtccagcaa gctgtatgag ccctaactgt ttgaatacca
     1981 gtagattacc agaaacttct atcgattgaa gacattacca actagcgtta ctaaatgttg
     2041 accaagtttt gttcacttgt ctggttttga tagtagcatt ctgcagagta ttttgatatg
     2101 ataaatatgc attaacaatg atattattgt taatttccta gcatgaaa
//
LOCUS       DAZPCS010000310         2144 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZPCS010000310 DAZPCS010000000
VERSION     DAZPCS010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.316"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(240..459)
                     /gene="ymgF"
                     /locus_tag="ACHUQ7_004118"
                     /pseudo
     CDS             complement(240..459)
                     /gene="ymgF"
                     /locus_tag="ACHUQ7_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell division-associated protein YmgF"
     gene            complement(591..2114)
                     /locus_tag="ACHUQ7_004119"
     CDS             complement(591..2114)
                     /locus_tag="ACHUQ7_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFR1137261.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTADVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIASAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLGYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
BASE COUNT          639 a          470 c          450 g          585 t
ORIGIN      
        1 ttttcacctt attttcatga cattgatcac tttggggtga ctattttgtt taacaaataa
       61 ctcttcgaca tcaaaattat taatagttgc catggtaatt atttcggtga tatataaaaa
      121 caactgtgat aacaccaagg ttgacaacta tttgcgcaat aattcctttc cttcaatgat
      181 ccaggcgttc actgccagca acccattcag ccgcatactg acagtgagaa actgaaagat
      241 tattgctgat ccctgtggct aacgagtctt tctcttaaat cgattaacca gattgatcgc
      301 cagccaaaca agggcattaa gcaaatttat cgtcacaagc acttctccca gcccggtact
      361 gggttttgca ataaggatga ccaggagggt gatcagcata aatgcaatgg aaaagatgat
      421 atagagagtg aaataatcca gattattact attattcatg ctactaatgg caatcctgtc
      481 aaaactttca aacaattata gcaaacaaaa taatgaacaa tagtaacaag aggcaacgag
      541 ctaattagct gtctataatt acaccgcact atggccttac tgatcaattt tcactcaacc
      601 acaaccttca ccttcggttt agaaaggata attttgacca ggtcgatgta tgtaacaggt
      661 ttatagaaat aataaccctg ctgaaatgtg atattatttt ggttcagata gccaagttgt
      721 tctttcgttt cgacaccttc agcgacgata ctgatcgaaa gtttacgcgc cagatccaat
      781 acacaatcca gaattcgggt tgattctggg tctgcgttta cgcggccaac gaaactgtga
      841 tcaattttga tataatcaat atgcaggtca tgaagataag aaagccctga gtagccagta
      901 ccgaaatcat ccagcgcgat gacaaaacca ttttcatgca gaatgttcaa ccgctgaacc
      961 agactttcat caacattcaa tggctcacgc tcggtgactt ccagaacaag gtttaaatcg
     1021 cggcgggtaa aactgtcacg gaaatttaaa cactcgtcga caaacgtcgg cgaaataata
     1081 tgcgaagcgc taaaattaat tccaatatga aaaccttccg gcagtttact cgcgatagcg
     1141 ttcatctgtc tggcaacctg attcatcagg ctttgtgtaa gcggaacgat taatccggat
     1201 ttttcagcaa gtggaataaa tgccgcgggt gaaatatatc caccgtgagg ttgtttccag
     1261 cgggctaaca cctcaactcc ccgcaatgtc ccttcccgac catttaccac aggttggtaa
     1321 aaagggacga tctccccatt atttattgcc cgtcgcagga tctcttcagg cgttgcgctt
     1381 ttattgaaat atctatcaag caggaaggca gcggcgctgg cgattaacag aataaatatt
     1441 aaaatcccaa agcctttatc gataagtctg tagaagctaa agagtggcgg cgggttaaag
     1501 ataatagtga aatgatattt tgtggagtga accttctcgg tatagggata atgtccatta
     1561 agcgtcatca catcagccgt tggtccaata atgtcatccg ccacgcgtaa tacatacctt
     1621 acccctttca agggtacatt aagcgccccg cgaatatgtt gatcacttat cgtaaccaaa
     1681 atgcgcgtat ctgcaaattg gctctgatag agcaggatag gtaatctatt aacggtgtcg
     1741 attgccggag ccaacagtaa attactgtcc gggacaacag gaatacgaga cagcaggacc
     1801 cgattcccag gcagggatgt acaccagact attccctgtt tgagaatgat gattgtgcgc
     1861 aggtgaggtt tcagagctgc ttcagtgcca agctgatatt gctcctcgag gtcgcattcc
     1921 ttcccggcaa tgttaaccgc tgtttgagtc gcctgtgacg cttcttcaag tatatgattg
     1981 atattgttgg cggcatatct tgctcccgcc aggtactctg cttttgcgga ataccagagc
     2041 tggatgttca aaatagcgac accggtaata aacaagcata tcgcaacgag gatgggtatg
     2101 agtgtattgc gcatttgctt gtcccggcca aagggtttaa taca
//
LOCUS       DAZPCS010000311         2135 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZPCS010000311 DAZPCS010000000
VERSION     DAZPCS010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.317"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..105)
                     /locus_tag="ACHUQ7_004120"
     CDS             complement(<1..105)
                     /locus_tag="ACHUQ7_004120"
                     /inference="COORDINATES: protein motif:HMM:NF017860.4"
                     /note="In lambda-like phage, genes encoding the i-spanin
                     subunit Rz, and the o-spanin subunit Rz1 (this protein)
                     overlap; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system o-spanin lipoprotein Rz1"
                     /protein_id="HFR1137262.1"
                     /translation="MCHPQPKPPAPPAWAMMPPSNSLQLLDETFSVSGT"
     gene            complement(65..400)
                     /locus_tag="ACHUQ7_004121"
                     /pseudo
     CDS             complement(65..400)
                     /locus_tag="ACHUQ7_004121"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
     gene            complement(397..930)
                     /locus_tag="ACHUQ7_004122"
     CDS             complement(397..930)
                     /locus_tag="ACHUQ7_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFR1137263.1"
                     /translation="MSAKIKYGLSAAVLALIAAGASAPQILDQFLNEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVIPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDVGRDCRIRSNNCYGQ
                     VIRRDQESALACWGIDQ"
     gene            complement(996..1625)
                     /locus_tag="ACHUQ7_004123"
     CDS             complement(996..1625)
                     /locus_tag="ACHUQ7_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1137264.1"
                     /translation="MKHEEMNQRFNHLENEITELNKKLSALVSSEDENKRRDEHYAAI
                     YDYCHKVAHETFMKFLQEKFLPAALSEKEAAYLRPEYVITVNSAGEEEHKSDFIASAP
                     DKDQEPHRPFRVSCEEGEFVVYENGKPVRASHHHCLKIINLAIRCLKDENTRVMKRIG
                     RCMGYLQVAAEIEALASGADMDAAVREALLRDFNTPPLRKSLMTGSSRG"
     gene            complement(1629..1844)
                     /locus_tag="ACHUQ7_004124"
     CDS             complement(1629..1844)
                     /locus_tag="ACHUQ7_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFR1137265.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVRPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
BASE COUNT          546 a          568 c          471 g          550 t
ORIGIN      
        1 cgtcccggat accgagaacg tttcgtccag caactggaga gagttcgacg gtggcatcat
       61 tgcccatgcc ggaggcgctg gaggtttcgg ctgaggatgg cacagggcat tttcctttga
      121 cgagcacccg accaccatta tcaagcttgc gccgaagagc atcattttca gctttcgcat
      181 cagctaactc cttcgtgtat ttagcatcga gtgcatcagc atcacgctgg cgctgctgca
      241 tgtcagtaat ggttgcgttc gccagcttca gttctccggc gtttttgtcg cgctgctctt
      301 tgtaggtaat ggcgttatca cggtaatgat taacagccca tgacaggcag acgatgatgc
      361 agataaccag agcggagata atcgcggtga ctctgctcat tgatctatcc cccaacaggc
      421 taatgcgctt tcctggtcac gacgaataac ctgtccatag cagttatttg aacgtatgcg
      481 gcaatcgcgc ccaacatctt ttatccacca gcgaatcgcc tcgcatgcgc ccttacgatc
      541 accggcattc agccgcttat aaaacgtcga cgggaaacac ttaccggggc caatgttata
      601 ggggcaaaat gacgctatac ccgctttctg gggttcggtc agaggtactt taatattgcg
      661 ctccacccat gccagcgcct tatcacgttc aatagcgtta acctggtcac atttttcctt
      721 cgacagcttc attcccggta tgacaggctt accatccacc attgtggcac cacgacagat
      781 ggtccatatg ccagaaccat cacggtatgc cgttgtgtga ttaccctctt tttcgttcag
      841 aaactggtca agtatctggg gagcagacgc gcctgcggca atcagcgcca gaacagcagc
      901 cgacaggccg tatttgattt ttgcgctcat ggatatttat caggatttat cggcaacaaa
      961 taacgagcca acttatatac gccttttaag ataagtcagc cccggctgga tccagtcatc
     1021 aggctctttc ttaaaggggg agtattgaaa tcacgaagaa gagcctcccg caccgcagca
     1081 tccatgtctg caccactggc aagtgcttca atctctgccg ccacctgcaa ataccccatg
     1141 caacggccaa tgcgtttcat aactctggtg ttttcgtctt tcaggcatct gatagcgaga
     1201 ttaataattt tcaggcagtg atgatgcgat gcccggacag gttttccgtt ctcataaacg
     1261 acgaactcac cctcttcaca ggaaaccctg aaaggccgat ggggttcctg gtctttatcc
     1321 ggtgcagacg caataaaatc acttttatgc tcttcctctc cggcactgtt aaccgtaatg
     1381 acgtactcag gccgcaggta agccgcctct ttttctgaca atgcggccgg taaaaacttt
     1441 tcctgcaaaa acttcataaa agtctcgtga gcgactttgt ggcagtaatc gtaaatcgcc
     1501 gcataatgct catcgcggcg tttgttttca tcttcagaac tcaccaacgc cgacaatttt
     1561 ttattaagct cagtgatttc attttccagg tgattgaatc gctgattcat ttcttcatgc
     1621 ttcattattt actctccccg tgcagcctta cgcttgtctt ctcttatttt gaaatacaga
     1681 ttcgtcagat aagtcagaag ccccagaagc agacttccca gcacaccaat cgcagcccac
     1741 tgtgatggcc tgacctgatc caaccactgc aaaaaccagt agccggcact gccggcggag
     1801 gtgccgtagg caatgcccgt tgaaattttg tccatggatt tcatagcctc acctccgcaa
     1861 ataacggatg gcgtagtttt acactgagaa atgaaaggga tttgaaaaga aaaaaacgca
     1921 aaagcgggcg aaacgatata tacagtaagg aaagcactct atccaacaaa ccacccacag
     1981 ttaatcggaa taaaagcaga gtgcttatga atgatcgcac gcccgaaggt tagtatttct
     2041 gcacagcaat tttgcaaaaa aaggcgatca ttcataactt aaacgtcttt cagtcactcc
     2101 gggatttccc atcatcgcag actgaaagac tctga
//
LOCUS       DAZPCS010000312         2089 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZPCS010000312 DAZPCS010000000
VERSION     DAZPCS010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.318"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(60..1025)
                     /locus_tag="ACHUQ7_004125"
     CDS             complement(60..1025)
                     /locus_tag="ACHUQ7_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFR1137266.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQIAAEFERYALRM
                     ASCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIV
                     VDYPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRG
                     KDGSAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGE
                     VVANVAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQL
                     DRETNEDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
     gene            complement(1879..>2089)
                     /locus_tag="ACHUQ7_004126"
     CDS             complement(1879..>2089)
                     /locus_tag="ACHUQ7_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1137267.1"
                     /translation="RSEAVIPDRFKNLILLIDGLSFGTFGFHPHEGFEDELILYIQKT
                     NERVKTLFVKIDLNGIKFGFLRTHS"
BASE COUNT          578 a          549 c          436 g          526 t
ORIGIN      
        1 aaaaaaaata aaccaatagt cttatttcca ttcaggtatt caatattaat attcatacgt
       61 taatccgatt tatccttctc aggggcgcgt ttgtaacgcc gtttacctga atcccagaca
      121 aacgccgtcc gcttcccgtc atcagtgcca tcccctacat cgtcggcaat gacaagatcc
      181 tcattggttt ctcgatccaa ctggaggacg ttttttagcg ccccaccggt cgagataaaa
      241 cggcgcttgt gaagctgccg cgtcagctca agaaaccact caggatcgtt tgccatatct
      301 tccggtttaa cggagtattt cagcgtttca gcaaccgcgc tttgcagttg ctcggcaacg
      361 ttggccacaa cctcacctgt cttagttttt actgcccgaa tatcgatatt cggctcatag
      421 ttcacccgca agcaatcgcg ccagagttct acccaacgtt cgtgcttaac gtagttcttc
      481 cctttaaacc aagaaggttg caccatcagc agacagtgaa aatgcggatg tgcgctgcca
      541 tccttacctc gcgtcacctc cgtagccctg atccacccct gaacaggtga tagctccttt
      601 cgcttttcca ttcgcttaaa cgccgcattc attgctgtaa ggactgttcc aagttcacct
      661 atctcgcagt tcctgacagt taacgtcaga aacaaccatc gggaagacgg gtaatccaca
      721 acgattttcg gtagagcctg ataaaaacgg gcttgccaca tgagggttct tctccactgg
      781 cagacagggc aatgacggac acggcaaaat tgcgcactac gaagccgtag gcgcgtttct
      841 ccggtttcca tgatggtaga ccaaccaaaa cgtaacaaac cgctacagga ggccatacgg
      901 agtgcatagc gctcgaattc agcagcaatc tggtagatac ccccgacaga atcggtaagc
      961 gtgcgctggg tatcccaaac tgcatcacgg gggctgtagt ccgaaaggaa tttatcttct
     1021 gacattaagc ctgctccgat ctgggcaggc tgtgatttga tgtggtatat tcatcacgta
     1081 cctgtgcatt gcgagccagt acatgtcacc agagtttgcc gctgaggtga cacattcgaa
     1141 atgttgggga gtgttgacgc actccccaac gccgattaaa aacgcctcaa ttctgctcca
     1201 ttttctttga cctgtcaaaa cccactcaaa gcctcgtcat tactgggcta aaccactatt
     1261 tcatatccgc catttatccc ttaagtataa atacaaggat cacgcgcgag acttgcgcaa
     1321 aaaataacca caattttaac cccccaaaac aagcactccc gaacggctga acccaacctt
     1381 ttccacacac ccgatcacca aaacagggaa taaaaacaaa accctgtctt acaggtggat
     1441 ggcctcactt cgtgaaacca tccaccccgc ataagttttt taatcggtgg tgtcgtcaat
     1501 cactaaatcg gaaccccaaa ggggaccccc acgatttagc atttgacgag attaactctc
     1561 ggagatggca tcatgctttt aacggacttt aaaaaacggt gaacggatgc ggattttaat
     1621 ccgtgtctgt gaggcgttga gcaacgagca aagcgaggcg gtagaaagag agccagcggc
     1681 aggctcagga cgataatccg gggaaaatgt accactcgca gagtgttaac atttgacatc
     1741 ggattgtcta tcctgcattg gtgtcgagtt ggcaaggctg aggataatgc tgaggataat
     1801 gcaggataga cagagcacta gagattgcat gcaatctcgt acctatcacc tcagcctgcc
     1861 gctggctctt ttccagaact aagaatgcgt tcttaaaaat ccaaacttga taccgtttaa
     1921 atctattttc acaaaaagag tttttaccct ctcgtttgtt ttctgaatat ataaaattaa
     1981 ttcatcctca aaaccttcgt gcggatgaaa accaaatgtg ccaaaactta atccatcaat
     2041 aagcaaaata aggtttttaa acctgtcagg aatcaccgct tcacttctt
//
LOCUS       DAZPCS010000313         2085 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZPCS010000313 DAZPCS010000000
VERSION     DAZPCS010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.319"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            1..797
                     /locus_tag="ACHUQ7_004127"
                     /pseudo
     CDS             1..797
                     /locus_tag="ACHUQ7_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608690.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            912..1253
                     /locus_tag="ACHUQ7_004128"
                     /pseudo
     CDS             912..1253
                     /locus_tag="ACHUQ7_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            1236..2073
                     /locus_tag="ACHUQ7_004129"
                     /pseudo
     CDS             1236..2073
                     /locus_tag="ACHUQ7_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          515 a          504 c          631 g          435 t
ORIGIN      
        1 tgggccttgc ccgtagcagt tatttttatc accgggctgc actgaaagcc ggtgataaat
       61 acgccacgat acgtaagatg ctgacagata tctttaacag taattaccag tgttatggct
      121 atcgtcgcct gcatgcgatg ctcaggcatg agggggggcg gctatcagaa aaggttgtac
      181 gcagacttat ggtggaggaa cagcttgtcg tcagtcgtaa ccgtcgtcgt cgctacagct
      241 catattgcgg agaaatcgga ccggctccgg ataaccttat cgccagagat tttaaggcgg
      301 agcaacctaa tcagaaatag ctgacagata tcacggagtt ccagctccct gcaggtaaag
      361 tctggctgtc accagtggtg gactgcttcg atggaaaagt tgtgagctgg tctctcagta
      421 cacgccctga tgctgaactg gtcaacacta tgctggatag cgctgtcgaa acgttaaatg
      481 ctggcgaacg accggtgata cacagtgaca gaggtgggca ttatcgctgg ccaggctggc
      541 tggaaagagt gaatgcagca ggtcttattc gctcaatgtc ccgtaaagga tgttcacctg
      601 ataatgctgc atgcgaaggc tttttcggca gactgaaaac ggaaatgtat tatgggcgta
      661 aatggtcggg catcacgcca gaaaagttca tgcagcaagt ggatgcttac atcagatggt
      721 ataacgagcg gcgtataaaa ttatcgctgg gtgccgtcag ccccaaaatg taccgccaac
      781 aatgcgggct ggaatgataa agcagtccag gaaatcgtcc gcatcccccc ttcaccattt
      841 ggattgtccg ctccacccaa catgttgttt ccttaaggtt cctacaccac aaaggaaact
      901 ctcatgctgc gcagtggatg gctgacgttg ccgacaggcg ggaacttcgc cagttcaggc
      961 agacaccgga acagcgcttc acgcaggaac aggagcatct gcagccgctg ctgggtacgg
     1021 acttcgatat ccgtcatgtc tcctgggatg gctatatcga ggttggcggg aaccgttaca
     1081 gtgtgccgga aagcctgtgt ggtcagctgg tatcaatacg gatctcgctg gatgatgaac
     1141 tgcggatcta cagtaatgag caacaggtag catcgcaccg tctctgttcg gctgcatacg
     1201 gctggcagac cgtgccggag catcacgctc cactctggca gcaggcttcg gagcgcatgg
     1261 ccgaatggct gggggagata cagaaacggg taatcactgt ctgtgaccgg gaagcggata
     1321 tctggcatta cctgtattat aaagtcagcc acggacagcg tggtgcgtgc tgcacagaat
     1381 cgccggctgg aagaggcacc cggcaagctc ttcgaactgc cggaagtcct ggcaaccgca
     1441 ggaagccaca cgctgaatgt gatgcaaaaa ggcgggcggg cagcccgtca ggcccggatg
     1501 ttcatcagtt acagcgaagt cagcataaaa aatcccgaca acagcggcca ggcgctcccg
     1561 ctcacgtatg tctgttgccg ggagcaggca gaggacggtg cctgctggca tctgctgacg
     1621 tcaggaaaag cggcgagtgc cgccgatgca cgacgtattg tcagccatta cgagcgacgc
     1681 tggctgacag aggaatacca caaggcgtgg aaaagtggtg gtacatggaa tcgctgcgaa
     1741 tgcagacccg ggataacctt gagcgcatgg tggttatcct ggcgtttata gcggtacggg
     1801 tgctgggcct gcggcaggga ggtgtcagtg aagaaacaca aaatgacagc tgtgagaaaa
     1861 tactgacgcc gacagagtgg aaactactgt gggttaagct ggaaggaaag ccgcttccgg
     1921 tgcaggcacc gaccctgaaa tgggcctggg gcgatggcat gacagcaaac gcacaggtcg
     1981 tcccggttgg ggcgtcatgt gggatggctg gtcagacttc aggatatggt tgagggttac
     2041 ctggtgatga agtctcttga tcaggagctc tgatcaagag acagc
//
LOCUS       DAZPCS010000314         2051 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZPCS010000314 DAZPCS010000000
VERSION     DAZPCS010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.320"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            1..347
                     /locus_tag="ACHUQ7_004130"
                     /pseudo
     CDS             1..347
                     /locus_tag="ACHUQ7_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            335..1633
                     /gene="ydeP"
                     /locus_tag="ACHUQ7_004131"
                     /pseudo
     CDS             335..1633
                     /gene="ydeP"
                     /locus_tag="ACHUQ7_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
     gene            1881..>2051
                     /gene="safA"
                     /locus_tag="ACHUQ7_004132"
     CDS             1881..>2051
                     /gene="safA"
                     /locus_tag="ACHUQ7_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFR1137268.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTH"
BASE COUNT          562 a          473 c          487 g          529 t
ORIGIN      
        1 ttgtagactt gtctcagcat ataagttgct ggaatgatta cggcggctgg tacgacactg
       61 atcatataaa cctggtacaa ggctcagctt tcgcaggctc cctacagtca tataaaggat
      121 cgctgtactg gaataatgtg acctacccct tcccgttgac gacaaatacc aacgtattgg
      181 atattggtga taagacaccg atgccattac cgttaaaact ctatattacc ccagttgggg
      241 cggccggtgg agttgtcata aaagcggggg aagtcattgc ccgtattcat atgtataaaa
      301 ttgcaacatt aggaagcgga aatccgcgta atgtgacgtt ctcaattccg agtggaaaga
      361 tatcgaacgt atttctggac taagtcagac acaaatcgcc gaactggctg acgcatatgc
      421 cgctgccgaa cgcaccatta tctgttacgg aatggggatc actcagcacg aacatggtac
      481 ccagaacgtg cagcaactgg tcaatttgct gttgatgaaa ggtaacattg gcaagcctgg
      541 tgcgggtatc tgcccactac gtggtcactc taatgtacag ggcgaccgaa ccgtcggtat
      601 caccgagaaa ccgtctgcag agtttctggc tcgcctgggt gagcgctatg gcttcccccc
      661 acctcatgca cctggacatg ctgcaattgc cagcatgcaa gcaatatgta cggggcaggc
      721 tcgagcattg atctgcatgg ggggcaactt tgcactggca atgccagatc gggaagcgag
      781 cgctgtaccg ttaacgcaat tagatttggc ggtacacgta gccactaagc ttaaccgctc
      841 tcatctgctg accgcacggc atagctatat tctgccggtt ctggggcgta gcgagattga
      901 tatgcaaaaa agcggtgcgc aggcggtaac cgttgaggat tcaatgtcga tgattcatgc
      961 ctcgcgtggc gtgttaaaac ccgccggtgt aatgctgaaa tcagagtgtg cagtggtcgc
     1021 gggaatcgcg caggcagcac taccccagag cgtggtagcc tgggagtatc tggtggaaga
     1081 ttatgatcgc attcgcaatg acattgaagc tgtgctgcca gagttcgccg actataacca
     1141 gcgcatccgt catcccggtg gttttcacct gataaatgca gctgctgaaa ggcgctggat
     1201 gacgccgtca ggtaaggcta atttcattac cagcaaaggg ctgttagaag atccctcttc
     1261 agcgtttaac agtaagctgg tcatggcgac agtacgcagc cacgatcagt acaacacgac
     1321 gatttatggt atggatgatc gctatcgagg ggtattcggt caacgagatg tggtctttat
     1381 gagtgctaaa caagctaaaa tttgccgtgt aaaaaacggc gaaagagtta atcttattgc
     1441 gcttacgcca gacggtaagc gcagctcacg ccgcatggat agattaaaag tggtcattta
     1501 ccctatggct gaccgctcac tggtgaccta ttttccagaa tcgaatcaca tgctaacact
     1561 tgataaccac gatccattaa gtggtattcc tggctataaa agtattccgg ttgaattaga
     1621 accatcaaat taatgcctct tctcatttct tctgctgtca tccgcacagc agaagaattc
     1681 ctcattgact attattgcgc aatttgctca catggattaa attaaactac atactataag
     1741 atataaactt ctgcctacag ctgtaagaaa ctccgctcag tactgaagca ccagtcctat
     1801 ttcttctttt ctccagcctg ttatattaag catactgatt aacgactttt aacgttatcc
     1861 gctaaataaa catatttgaa atgcatgcga ccacagtgaa aaacaaaatc acgcaaagag
     1921 ataactataa agaaatcatg tctgtaattg tggttgtctt attactgaca cttacgttga
     1981 tagccatttt ttcggcaatt gatcagctga gtatttcaga aatgggtcgc attgcaagag
     2041 atcttacaca t
//
LOCUS       DAZPCS010000315         2040 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZPCS010000315 DAZPCS010000000
VERSION     DAZPCS010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.321"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(13..901)
                     /locus_tag="ACHUQ7_004133"
                     /pseudo
     CDS             complement(13..901)
                     /locus_tag="ACHUQ7_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
BASE COUNT          551 a          475 c          474 g          540 t
ORIGIN      
        1 gtattaagac tatcacttaa ataagtgata ctggttgtct ggagattcag ggggccagtc
       61 tagcagctgc tctacggtca tatttttaat tgcgctccgg tttacaagag cccacccctg
      121 tttttccaga taaaactgga aagtatccag ggtacagacc agtgcaccgt caggaacagt
      181 ttcggtgaat ctgatattgc cgtgttcgtc gaagtgcaca accagagtgc gaccgttacc
      241 cggaatcatc ttgtcagcgg acggggtatt attctggtgc agttctgcct ccatgcggtc
      301 gaacatcagc gatgtaggcc tctttgaatg cagcggcttt tttcccagtg aagcccatca
      361 ccaggaaaac gaagccgttt ttggtgattt ggtacattgg gcgtttttcg cctttggtgt
      421 cggtgtaggt gacgtcctca aaattgaggg cgttaaattc aggtgagcat tcagtatttg
      481 cgatggcacg taacacattg tcgtgtcgtt tgtggaagaa ctctgcaacc gcaacagacg
      541 tagtgacagc gcgaccgttt tcgatggtta cgtcagggtg agaaagggca gggatagtag
      601 ccatgatggc atcctcgagt gataagttaa ttaactcacc accgaggttt tccacgacca
      661 taagggtggt gagacgtaca ggggtggaaa taccggtcac tcgagaaccc ggccagtctt
      721 gcgactgccc tgcacatccc accataattt gaatggagct gtgcattacg cataaaaaaa
      781 ccgcttcagc gcggctatgc gctcgagtaa cgttcgggtt tccacgcccg gcccccgttt
      841 tatgaggtgc agatgcacta taattccacc cgttctggtt ttcaatagct acattcaaca
      901 ttttctctta cctttcatca ccgaagtgaa ctttgttgat gcggtgcctg gtgcctccag
      961 gtgacgttaa ccagttaaca attaacgccg gatacaggga aacccataac cacccgatac
     1021 gtgggaacta atcgcttttt aactgttccg cgtgcgctca gccgcattca ccgcatcaca
     1081 aaattcactt taaaaagggc ggacatcagc cagcaatgaa actgatgccg ccaaaggtac
     1141 caatcaacat ggagtgttgt ggcgtggttg tcacttaagc gtatggtcaa cctgacaacc
     1201 cggtgtcctc aacggggaag gaataactcc gccatactta ccgccgcgtc atttcgcgtt
     1261 gtgtgcctgc ttttaactac gtcaggcgag gtggttcctg ttattcccca acaacaagaa
     1321 atttgtataa tctgaatacc ccaacaataa gaagagtgag caagtgatgt attagcgatg
     1381 tcagctctca aagagactgc atcaatgtta aaattattca atgaagcaaa aactcaggct
     1441 gaggttgatc gcgctgtgtc tgaaataaac tctaagttgt caagcattca gcttgaatat
     1501 ttgtcatctc attgatgagc tgacaatctc taaaagcaat gaggctgcgc tcaaagcaaa
     1561 actcacggag atagaaaagt ttaagttcca aagtgaagac tgcgaacctc gctaattgat
     1621 tactggaacg attgtgtatg ccagacagtg caatactagc tctggcaaga ttaccatgta
     1681 tttttgtcca gaatgttttg cgaagagagc aatatctata ttgcaacctt tccctatcga
     1741 ggaaggcgat aaatatacga aaactcaatg cccctcctgt aaaaatgagt atctcatgga
     1801 ctacaatttc aataatgatt tttgagtcac ggtattcaga tttttaaaga acatgccgga
     1861 tactcacccg tgtccggcgc acgcactcca cctcacccgt ggagaactcc ttaattacca
     1921 accttggctt cgttggttag ccattaacgc tggtatgtaa tcattctggc aatgcttaat
     1981 ttgaagtggc acactgaatt tggccacctg aacagaggtg atatgctcac ctcagaacaa
//
LOCUS       DAZPCS010000316         2032 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZPCS010000316 DAZPCS010000000
VERSION     DAZPCS010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.322"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..1824)
                     /locus_tag="ACHUQ7_004134"
     CDS             complement(<1..1824)
                     /locus_tag="ACHUQ7_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFR1137269.1"
                     /translation="MRPSELSDLLWAQVDRVAPHLLPNGKIEGHEWVAGNVNGDKGNS
                     LKVNLIGKKKWADFAEGDGGDMLDLWMACRGINLHQAMQEAKAFLGIKDDDHHFDARR
                     EKKFSRPDRKKIARYVTRTESHLEYLQSRGISPEVVKRYEVVSGKVWNGERELDALVL
                     PYKRDGELLQVKRISTERPDGKKVIMAEGDCEPCLFGWQALDAGVRVVVLCEGEIDCM
                     SYAQYGISALSVPFGGGKGAKQQWIEFEYHNLDRFEEIFISMDVDDVGREAAREIVSR
                     LGEHRCRLVTLPYKDINECLMNGVTEDEIWQYIGTASYFDPEELYSAREFYQDTINAF
                     YGKQQYLFNPPWESLADKFQFREAELTLVNGVHGHGKACPLNEPILLADGTWTTHGNV
                     KIGDQVASVDGNPSTVTGIFPQGVRDVYRVTFEDGRYVDCAGDHLWEVTSRGFTKGEK
                     RRVIDTFGLKRLSETKRHKNGVRIPEITGDFGDHSEPLAWVIGSLLGDGSLSNGSVKF
                     SNVEPYMIERMKAELPDYNFSGDGKDWLISTARGQVNPLMETLRGYGLMGCTAKNKFI
                     PRVFFSANKSTRIGMLCGLLETDGYVEKDGTLVFSSASEELR"
     gene            complement(1932..>2032)
                     /locus_tag="ACHUQ7_004135"
     CDS             complement(1932..>2032)
                     /locus_tag="ACHUQ7_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1137270.1"
                     /translation="RWNDEKPETIQPQSRPSSAITVSKTGYVFFDR"
BASE COUNT          485 a          580 c          431 g          536 t
ORIGIN      
        1 gcgcagttct tcacttgctg aggaaaaaac aagcgttcca tccttctcga catacccatc
       61 cgtttcaagc agaccacaca gcatgcctat acgcgttgat ttatttgcgg aaaaaaacac
      121 acgagggatg aatttgtttt ttgctgtgca ccccattagt ccataacctc gcagggtctc
      181 catgagtgga tttacctgac cacgcgccgt tgatatcagc cagtccttac catctccaga
      241 gaagttgtaa tcaggcagtt cagccttcat acgctcgatc atgtatggct cgacgtttga
      301 aaacttcacg ctcccattgc taagactacc atccccgaga agggagccga taacccatgc
      361 taatggctct gagtggtcgc caaagtcacc agttatttca ggaatcctaa cgccattttt
      421 gtgcctcttc gtttcactca accgcttcaa cccgaaggta tctatcacgc ggcgtttctc
      481 gcctttcgtg aatccacgac tagtgacctc ccataggtga tcgcctgcgc aatcaacata
      541 acgaccatct tcaaatgtga ctcggtaaac atctctaaca ccctgcggga atatcccagt
      601 gacagttgac ggattgccgt ctactgacgc cacctgatcg ccaattttta cattcccgtg
      661 agtagtccat gtcccatcag ctaaaagaat aggctcattc agtgggcatg cctttccgtg
      721 accgtgcaca ccattgacca gcgtcaactc tgcctcacgg aactggaatt tatctgccag
      781 agattcccac ggtggattaa acagatactg ctgcttgccg tagaaagcgt tgatagtgtc
      841 ctggtaaaac tctcgcgcac tgtagagttc ttcaggatcg aagtaggatg ccgtgccgat
      901 gtactgccag atttcatcct cggtaacacc gttcatcagg cattcgttga tgtctttgta
      961 cggcagagta acaagacggc aacgatgttc accgagtcgg cttacgattt cccttgcggc
     1021 ttcacgacca acatcatcaa cgtccatcga gatgaatatt tcctcaaacc tgtcgaggtt
     1081 gtgatactca aactcaatcc actgttgctt agcgcctttc ccgccaccaa acggcacgga
     1141 taacgccgag atgccgtatt gcgcatagct catacaatca atttcgcctt cgcaaagcac
     1201 aaccaccctc acgccagcgt ccagagcctg ccatccgaac aggcaaggtt cgcaatcacc
     1261 ttctgccata atgactttct tcccgtccgg gcgctcagtg ctgattcgct tgacctgcaa
     1321 caactcacca tcgcgtttgt acggaagcac cagagcatcc agttctcgtt ctccattcca
     1381 caccttgccg ctgacaacct cgtagcgctt tacgacttct ggcgatatgc cacgcgattg
     1441 caggtactca agatgggatt ctgttctggt aacgtagcgg gcgattttct tacgatcagg
     1501 tctggagaat ttcttctcac gtctggcatc gaaatggtga tcgtcatcct tgataccgag
     1561 aaatgctttc gcttcctgca tagcctgatg caggttaatt ccacgacatg ccatccacaa
     1621 atcaagcatg tcaccgccgt ctccctcagc gaaatcagcc cattttttct tgccgataag
     1681 gttgacctta aggctgtttc ccttgtcacc gttgacgtta ccggcaaccc actcatgccc
     1741 ctctatcttg ccgtttggca acaggtgcgg agccaccctg tcaacctgcg cccaaagcag
     1801 gtcgctaagt tcacttggcc tcatgattcc ctcagattga gatttttaaa ccagaaatcg
     1861 acaaacgaaa tacttaacca gccgtggtta taaccagcga ccagtagcga tttgattttt
     1921 gatttcatgg ttcacctgtc gaaaaacaca tagccagttt tcgatacggt gattgcggat
     1981 gatggtctgg attgtggttg aatggtttct ggcttctcgt cgttccagcg ct
//
LOCUS       DAZPCS010000317         2031 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZPCS010000317 DAZPCS010000000
VERSION     DAZPCS010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.323"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(91..1503)
                     /gene="pykF"
                     /locus_tag="ACHUQ7_004136"
     CDS             complement(91..1503)
                     /gene="pykF"
                     /locus_tag="ACHUQ7_004136"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFR1137271.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            complement(1511..1705)
                     /locus_tag="ACHUQ7_004137"
     CDS             complement(1511..1705)
                     /locus_tag="ACHUQ7_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1137272.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
BASE COUNT          510 a          455 c          490 g          576 t
ORIGIN      
        1 tttgattttt tattgcttct ggttatcgat taaataaaaa aagcgcccat cagggcgctt
       61 cgatatacaa attaattcac aaaagcaata ttacaggacg tgaacagatg cggtgttagt
      121 agtgccgctc ggtaccagtg caccagaaac cataactacg acgtcacctt tgtgtgccag
      181 accgctctgc agagccagtt ctttacccag acggtagaaa tcatcagtag aagtgatctc
      241 tttaaccagc tgcggcacaa cgcctttgct cagtaccaac tgatgagccg ttttttcgtt
      301 ggtggtcagt gccaggatgg tggcatccgg gaagtattta cgtactgcgc gagcagattt
      361 accgccctgg gtagcaacca cgatcagcgg agcatccagt ttttcagcag tttcaacggc
      421 accacggcat accgcttcgg tgatgcgcag tttacggttg tcattgttga actcgagacg
      481 gctgttcatc acgcggtcgg tacgttcgca gatggtcgcc atgatagaaa ccgcttccag
      541 cgggtattta ccttttgcgg attcaccaga cagcatcact gcgtcagtac cgtcgaggat
      601 ggcgtttgca acgtcaccgg cttctgcgcg agtcgggcgt gggtttttga tcatggaatc
      661 cagcatctgg gtcgcagtga taacgacttt acgtgcacgg atacattttt cgatcatcat
      721 cttctgggcg aagataactt cttctaccgg gatttctaca cccaggtcgc cacgcgcaac
      781 catgatgccg tcagaggctt cgaggatttc gtcgaagttg ttgaggcctt cctggttttc
      841 gattttggag atgatgtgga tgttttcgcc gccgtgcgct ttcaggtgct cacggatttc
      901 gataacgtca gaacgcttac gaataaagga agcagcaaca aagtctacgc ctttttcgca
      961 accaaagatc aggtcctgtt tgtctttttc agccagtgct ggcagagcaa tggaaacgcc
     1021 aggcaggttc acacctttgt tttcgcccag gtcaccgttg ttcagcactt tacagataac
     1081 tttgttacct tcaatggcgg taacttccat accgatcaga ccatcgtcaa ccagtacggt
     1141 gttgccaaca gacaggtcag tagtgaaacc ttcatacgtt accgcaacca tttcgctgtt
     1201 gccgataaca gatttatcag tggtgaaagt aaaggtctga ccagctttca gagaaacatc
     1261 gttaccgcct tccagtttca tggtgcggat ttccggacct ttggtatcaa gcaggatagc
     1321 ggcggtttta ccagttttgc tcatcacgtt gcgcagattc tgaatgcgct gaccgtgttc
     1381 tgcatagtca ccatgagaga agttcagacg cataacgttc atgccagcgt ccagcatttt
     1441 agctaacatc tcttcagatt cggttttcgg tccgatggtg caaacaattt tggtcttttt
     1501 catgacagtc ttagtcttta agttgagaag gatgggagaa acttgctttc tgggcgcact
     1561 gccggaaagc gaatccggta ttacgaaagt ggtggtgcct cgctctaagg ataggtgaca
     1621 tcgaaaaagc gtgcagagga atgtgtgctt gtgtctcagc ccaacgtgag tgtctgtttt
     1681 tgagtcgttg agttctacag tccaaagtgc tgaaaccatt caagagtcaa ttggcgcgca
     1741 ttatacgctg aaattaatcc aaaaggaaaa caaaaacagc gtttcaaaaa atatacaact
     1801 tgttagacaa taaattgtta tcaaagattt aacgttccag ggaaaaggtt acgcaaaaac
     1861 ttcgctgttt gcgcaacgtc tggtgccgaa gttgtcagtg tgaggataag tgaaagttca
     1921 tccggtgtat aaatatatca tcgctcaatt gctacagtta aaataattta tcgcgttatt
     1981 tgtctgattt tgtttgattt ttgtttaatt ggttagattg atatatgctg a
//
LOCUS       DAZPCS010000318         2005 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZPCS010000318 DAZPCS010000000
VERSION     DAZPCS010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.324"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..494)
                     /locus_tag="ACHUQ7_004138"
     CDS             complement(<1..494)
                     /locus_tag="ACHUQ7_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HFR1137273.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSA"
     gene            complement(525..1217)
                     /gene="fimC"
                     /locus_tag="ACHUQ7_004139"
     CDS             complement(525..1217)
                     /gene="fimC"
                     /locus_tag="ACHUQ7_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFR1137274.1"
                     /translation="MMTKIKLLMLMIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIKNSHTNELFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(1437..1979)
                     /gene="fimA"
                     /locus_tag="ACHUQ7_004140"
     CDS             complement(1437..1979)
                     /gene="fimA"
                     /locus_tag="ACHUQ7_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFR1137275.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIVSSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
BASE COUNT          584 a          422 c          427 g          572 t
ORIGIN      
        1 tgagcgctac tcaacaacgc tatctgagga atagtgatgt tcaggcgcat cgctgcaaaa
       61 tcaaagttaa ttgtggcatc aggtacagct ttcagtaaat tgatgcattt attgttttgc
      121 tgctgggcta actcgggaaa tgcactgcta ttaaggccaa ttcgttcaag gagtacctgg
      181 ttaaaacagg gcattaaccc acctgattta tcacctgtat tttctgttgt cgattcaaat
      241 acgatatcct gtgaaccaat gaactcatca ttacgccaga gatccacccg atacacgccc
      301 gcaggttgat gattacctct ctcaaagcgc gataaatcag caacggatgt gccattctct
      361 aataaaaatg ccgggttgaa ataactttcc gcatagctta aaaaggaggg taaaatggca
      421 agagatgaat aacaaattcc ggccagacac caggtatacc tctgcggaat atccgtagta
      481 gtgggtattt tcataattag ctctgatatt gtcagatgat tcggttagtt taagttcact
      541 tctctgaccg gggttactga accataatca ttaacggtct gaaatttcag ctttccctgc
      601 actcctgagg gtaagggaat ttgtgaacta actctgggtg ccgccatagc attccccaac
      661 ttttggctgc ccacttgcag gttaacgagg gtgacataaa aaggtgatgg attatgaaca
      721 ttgatatagt taccggatcg cgaaaacttg agtgtgtccg gcgcttctgc gggtaattct
      781 tgtaattgaa ttggacggag aaataatttc atgcgcgata aaatcgccag ttgcaaaaca
      841 ttcctgccgt tcaatgcatt tttatctacc gaagggatcg tcttaacatt catccagaac
      901 agagactcac gatctgccgc cagcggtgga ccggtgtaaa taatacgcaa agtattttcg
      961 cttttgggtt cactaacaaa tagtggcggt gtaatgatga atgacttttc ttttacaccg
     1021 ctgctgtttt caatccacga attgaccaga aagagctcat tggtatggct atttttaatc
     1081 cataccgcag tctgtttagc atcagcggga taaataatac gcgtggcacc taatgcgatc
     1141 cctccggcag catgggcgct ggccgaaatg attaaataaa atatcatgag catcaataac
     1201 tttattttag tcatcatagt taatacgcct tgtgtattcc tgacggcaaa aataaggagt
     1261 ataaagaaat gattgctttg gcttcttaca acacatcatt cccttttcat ttaataaagc
     1321 ggcatgatac aaagtgaacg tggtttcgat ttacattgcc accataccgc ttcagacaac
     1381 cggtacatta ctttgttata agacgtgata tataattcaa aacaacgtgg ttttgattat
     1441 tcgtatctca taataaaagt cgcgtcagca tttgcttgcc ctgctgatgc acttgtaccg
     1501 gtgcctttat aacgtgcaga aaaatgaaga acgttggtcc cggggatcaa gttctggttg
     1561 gttgagaagc tattcccatc aggtttgaga attgcggatg tattatcaag tatttcaata
     1621 ccgacacccg ttgctgttgt tgtatttgta ctggatacaa tggccaataa attatcattg
     1681 attgcatcag cctgaccaga aaatgcaacg gcagcattag cggcaacaac aggatcgcag
     1741 tcgttcaact gaatggtgaa tggaattaat gcagaggtat taccaacagc attgaaaata
     1801 tcggtacgat attgaccgag tgtgacaacc tggtctgccg agtcagtatt cactgaacag
     1861 gcagcattca ccagttcgcc ttcaaagtga attgtaccgc catctacaac ggcagcataa
     1921 ctacccgtag cggcgaatag tgcggcagcc agcgcagacg aaataaatct taatttcata
     1981 tatattcctt caatctcatt tatcg
//
LOCUS       DAZPCS010000319         1979 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZPCS010000319 DAZPCS010000000
VERSION     DAZPCS010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.325"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..857
                     /gene="virK"
                     /locus_tag="ACHUQ7_004141"
     CDS             <1..857
                     /gene="virK"
                     /locus_tag="ACHUQ7_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HFR1137276.1"
                     /translation="RFMYILRCSINPVVSIRYYYELRSLPCIEDILAIHPTLPARIHR
                     PYLHKGGRAWTRGQYILEHYRFVQNLPEKYSKFLFPQKSVSLVQFIGKDGENFDIQCS
                     PSGFDREGELMLSLFYNKTVIARLTFSVILTQNGHIAFIGGLQGAPKNTGPDVIRCAT
                     RACYGIFPKRIIFEAFCALMKACNISECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDF
                     WESVAGKTCGDWYRLPTQVIRKPLSDIASKKRSGYRKRYALLDYIHETTIRSLDAYPV
                     HSEHQNLN"
     gene            922..1866
                     /gene="lpxM"
                     /locus_tag="ACHUQ7_004142"
                     /gene_synonym="msbB"
     CDS             922..1866
                     /gene="lpxM"
                     /locus_tag="ACHUQ7_004142"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFR1137277.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWSTWFLLGMIAGISMFPPSFRDP
                     VLAKIGRWAGRLSKKARRRATINLSLCFPEKSDTEREIIVDKMFVTALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGKKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHAREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHLLTIEIRPPMDACIASADNKTI
                     ARQMNKTVEILVGPHPEQYVWVLKLLKTRKSNEADPYP"
BASE COUNT          580 a          382 c          446 g          571 t
ORIGIN      
        1 ttcggttcat gtacattttg cgatgttcta taaatccggt tgtcagtatt cgatattatt
       61 atgaactgcg ttccttgccg tgcattgagg atattctggc aatacacccc acgttgccag
      121 caagaattca tcgcccttat ttgcataaag gggggcgcgc ctggacccgt ggacaatata
      181 ttcttgagca ttatcgcttt gtgcaaaatt tgccagaaaa atattctaaa tttttgtttc
      241 cacaaaaatc ggtatcatta gtccaattca tcgggaaaga tggagagaat tttgatatcc
      301 agtgttctcc cagtggtttt gaccgggaag gagagttgat gttgtcattg ttttacaata
      361 aaacagttat tgcccggtta acattttcag tcattctgac tcaaaatgga cacattgctt
      421 ttattggcgg attacaggga gcccctaaaa ataccggacc tgatgttatt cggtgcgcaa
      481 cccgggcctg ttatgggatt ttcccaaaac ggattatttt tgaagcattt tgtgcgttga
      541 tgaaagcttg caatatctct gagtgtctgg cagtcagtga gcacagtcat gttttccggc
      601 aattgagata ctggtatcag aaacgcaaaa cctttgttgc ggtctatagt gatttctggg
      661 agtctgtagc aggaaaaacc tgtggtgact ggtacagatt accaacccag gtgattcgaa
      721 agccgctaag cgatatagcc agtaaaaaac gctctgggta ccgaaaacga tacgctttgc
      781 tggattatat ccatgaaact actatccgct ctttggatgc atatcctgta cactcagaac
      841 atcagaattt aaattaaggt ttgatgtatt ctctgaataa aacagtaatg ataattttgg
      901 tagaggcatt cgcactaaat aatgaaaaaa tataaatctg agtttattcc tgaatttaag
      961 aaaaattacc tttcccctgt ttactggtct acatggttcc ttttgggaat gattgcaggt
     1021 atttcaatgt ttcccccttc attcagagat cctgtcttgg caaaaatagg gcgttgggcg
     1081 ggcagattga gcaaaaaagc tcgtcgcagg gcgacgatta atttatcgct ttgtttccca
     1141 gaaaagagtg atacagaacg ggaaataatt gtcgacaaaa tgtttgtcac agcattgcaa
     1201 tccatagtga tgatggcaga actggcaatt cgtggtccgg aaaagttcca gaaacgagtg
     1261 ttctggaaag gcctcgaaat tcttgaggaa attcgacaca ataatagaaa tgtgattttt
     1321 ctggttcccc atggctggag cgtggatatt cctgcaatgt tgctggcagc tcagggaaaa
     1381 aaaatggccg ccatgtttca tcagcaacga aatccagtga ttgattatgt ctggaattca
     1441 gtacggcgta aattcggggg gcgcttacat gcccgggagg atgggataaa accatttatt
     1501 cagtcagtac gccagggata ctggggatat tacctcccag atcaggatca tggtcctgaa
     1561 tacagtgaat ttgctgattt ttttgcgacc tataaagcga cattaccaat cattggacgt
     1621 ctgatgaaca tcagtcaggc tatgattata ccacttttcc cggtttatga tgaaaaaaaa
     1681 catctactga ctattgaaat tcggccacca atggatgctt gcattgccag cgcggacaat
     1741 aaaacgattg cccgacaaat gaacaaaaca gtggaaattt tggtggggcc acatccggaa
     1801 cagtatgtct gggttttaaa attgttaaaa acacgcaaat caaacgaagc ggacccgtac
     1861 ccttgaaatt ataaagtaca agtatttcca ctagttgttt gtaagcgtac agcctgaacc
     1921 gtctggtcag aatctgacga attagacaaa gtggtgtcca ccaaataagt agtgggaac
//
LOCUS       DAZPCS010000320         1969 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZPCS010000320 DAZPCS010000000
VERSION     DAZPCS010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.326"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            240..509
                     /gene="yacA"
                     /locus_tag="ACHUQ7_004143"
                     /pseudo
     CDS             240..509
                     /gene="yacA"
                     /locus_tag="ACHUQ7_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015056448.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin YacA"
     gene            509..678
                     /gene="yacB"
                     /locus_tag="ACHUQ7_004144"
                     /pseudo
     CDS             509..678
                     /gene="yacB"
                     /locus_tag="ACHUQ7_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009346.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin YacB"
     gene            761..1351
                     /gene="ospG"
                     /locus_tag="ACHUQ7_004145"
     CDS             761..1351
                     /gene="ospG"
                     /locus_tag="ACHUQ7_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector protein
                     kinase OspG"
                     /protein_id="HFR1137278.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFED
                     VNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVP
                     GTPLSDIDTADIPDNIESLYLQLICKLNELSIIHYDLNTGNMLYDKESESLFPIDFRN
                     IYAEYYAATKKDKEIIDRRLQMRTNDFYSLLNRKYL"
     gene            1708..>1969
                     /locus_tag="ACHUQ7_004146"
     CDS             1708..>1969
                     /locus_tag="ACHUQ7_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR1137279.1"
                     /translation="MTEALRLHFEEHLPRVVAGCRLGVPKSTACGMFVRFRNAGLSWP
                     LPAGMSEQELDALLYGSASTVPVVLTESTVMPKLPVVKKRPRR"
BASE COUNT          560 a          433 c          462 g          514 t
ORIGIN      
        1 ctgaaaacgg ctcacaactg caccattcat aatcacatcg cataaaactg tcagcgccag
       61 tgataggaca ggtgaaacct gtcaatacgt accggcttac gccgggcttc ggcggtttta
      121 ctcctctgcc atatgcaaca acggagtgcc gccttacatg ccgctggcgc ggcatatcct
      181 ggtgacgata tctgaacagt tatatactgt gtatatacgt ggtaatgacg aggtggcaaa
      241 tggcacaggt taatatgagt gtaagaatcg acgcagaact gaaggatgct tttatggccg
      301 ctgcaaaaag catggaccgt aatggctctc agttaatccg ggattttatg cgccagaccg
      361 ttgaacggca gcacaattct tggttccgtg actaggttgc ggcaggccgt cagcaactcg
      421 aatgcggtga tgtgcttccc catgacatgg tcgaatcctc tgctgctgca tggcgggatg
      481 aaatgagcag gaagattgct gataaatgat ggagatgttc tggaccatgc tggccatcca
      541 ggacaggaag cgcattcgta gtacattgcg gagcagaacc tgatggccgc cattgaaccg
      601 gacgaacgga ttggttattc agccagcagc ctggcagggc agccgtataa aggccgcaac
      661 ggtcgtgtgg aaggaacatc tggaccacac aaggtagcgt gtaacgttat tttgtgtgaa
      721 aatctcttgt gatttttaaa tatcgtaaca aaggtatgac atgaaaataa catctaccat
      781 tattcaaaca ccttttccat ttgagaataa taattctcat gctggcatag taacggagcc
      841 cattctcggt aagttaatag gtcaggggtc gacagcagaa atctttgaag atgtgaatga
      901 ttcatctgct ttgtataaaa agtatgatct tattggcaac cagtacaatg agattctgga
      961 aatggcttgg caagaatctg agctttttaa tgctttttat ggcgatgaag catccgttgt
     1021 tatacagtat ggcggagatg tgtacctccg aatgctgcgc gtgcctggga ctccccttag
     1081 tgacattgat acagctgata tccctgataa tatagagagc ctttatctac agttgatatg
     1141 taaattgaat gagttgagta taatccatta cgatcttaat acaggtaata tgctgtatga
     1201 taaagaaagt gaaagtttat tcccaataga ttttcgcaat atttatgctg aatattacgc
     1261 tgcaaccaaa aaagataaag agattatcga ccgacgatta caaatgcgta caaatgattt
     1321 ttattcgtta ttaaacagga aatatttata gacgtatttg ttgatgctat aagccagagg
     1381 ctcttctatg agaatatggg gaccattccc tcgcgatatt aacgtaatat cttaattgta
     1441 tgcaccgcca gaatgcgttc agtgcgatca cggcagcaga caggtaaaaa tagcaacaaa
     1501 ccacccgaaa aaccgccgcg atcgcgtatg ataaatttta accacatgca tacctgtgca
     1561 acaaggcgaa tcacgcacgg atttccagcc agagacacct gaaacgtaac cggctcattt
     1621 aaaccgtctg gtctgttacc tccggttcca caaaaataat gtccatcatt ttgaagtgga
     1681 cactatcgta tggaataccg gacctgaatc actgaagctt tacgccttca tttcgaagaa
     1741 catttaccac gggttgtggc cggatgtcgc ctgggcgtac caaaatcaac tgcctgtggt
     1801 atgttcgtgc gcttccgcaa cgctggcctt tcatggcctc tgcccgcagg tatgtcggag
     1861 caggaacttg atgcccttct ttacggaagt gcctccacag tacctgtcgt acttactgaa
     1921 agcaccgtta tgcccaaact cccggtggta aaaaaacgtc cccggcggc
//
LOCUS       DAZPCS010000321         1944 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZPCS010000321 DAZPCS010000000
VERSION     DAZPCS010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.327"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..>1944
                     /locus_tag="ACHUQ7_004147"
     CDS             <1..>1944
                     /locus_tag="ACHUQ7_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HFR1137280.1"
                     /translation="CGVYPQDLKDREIISTFHAEKVINDLLKNDYKISLSPDTTYREL
                     NKAAQSSITAPDRIGEGKTWVYQRDTMVERGDNSGVHQYGPAEHFTHIISDKPSPKDK
                     YVAYAINIPDYELAADVYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQC
                     EIITPKKPGEAILHAFNATYQQIRENMSEFARSHYGYIQIPPVTTFRADGPETPEEEK
                     GYWFHAYQPEDLCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDL
                     DYPSADIRAKISKDIESGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEAS
                     KDNISKDEWKNLHALKEKSLNGMPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAK
                     HSKHEQIERSITPPLKNYNERLHNTNLFNPKYHEKTVMNYMEYLVTCQLNEEQMSSLL
                     YERDDLSAIDFSLLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNN
                     GNLFNDFLFNHQQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGPTRVGGEA
                     WLNKGCSTYQQLYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGL
                     VIINKGNEEVEGTWPNKLQPGIY"
BASE COUNT          680 a          383 c          366 g          515 t
ORIGIN      
        1 cttgtggagt ttatcctcag gatttaaaag acagagaaat tatatcaact tttcacgcag
       61 aaaaagtcat taatgatctg ttaaaaaacg attataaaat ttccctttct ccagatacaa
      121 cttaccgaga gctgaataaa gctgcacaga gtagcattac agcgccagac aggataggag
      181 aagggaaaac atgggtttat caacgagata caatggttga aagaggtgat aacagtggtg
      241 ttcatcagta tggtccagct gaacatttca ctcacattat atctgacaaa ccgtccccaa
      301 aagataagta tgttgcatat gctattaaca ttcctgacta tgagctagca gccgatgtat
      361 ataatattaa cgtgacgtca ccttccggac agcaagaaac atttaaaata ctaatcaatc
      421 cagaacatct acggcaaaca cttgagcgta aatctcttac tgctgttcag aaatcacaat
      481 gtgaaatcat cacccccaaa aaacctggcg aagcgattct tcatgctttt aatgccacct
      541 accagcaaat cagggaaaat atgtctgagt ttgcacgttc ccattatggg tatatacaaa
      601 tccctccagt gacaactttc cgcgccgacg gaccagaaac tcccgaagaa gaaaaaggtt
      661 actggtttca cgcttatcaa cccgaagatc tttgtaccat ccataatcca atgggggatt
      721 tgcaggattt tatcgcattg gttaaagatg ctaaaaaatt tggtatcgat atcattcctg
      781 attatacctt taactttatg ggaatcgggg gaagtggtaa aaatgacctg gattatccct
      841 ctgctgatat acgagcgaag atcagtaaag atatagaaag tggtatccct ggctattggc
      901 aaggtcaggt tttgattcca ttcattaaag atccagtaac aaaagaacgt aaacaaatcc
      961 atccagaaga tatacatctc actgcaaaag acttcgaagc aagtaaagat aacatctcta
     1021 aggatgaatg gaaaaacctc catgcattaa aagaaaagag tttaaatgga atgcctaaaa
     1081 caacccccaa aagtgaccag gttattatgt tgcaaaatca atacgttcgt gaaatgcgaa
     1141 aatatggcgt acgaggttta cgttatgatg cagcaaaaca ctcaaaacat gaacaaatag
     1201 aaagatcaat aaccccaccg cttaaaaatt ataatgagcg attacacaat actaacttat
     1261 ttaacccaaa atatcatgaa aaaaccgtta tgaattacat ggaatatctg gtaacttgtc
     1321 agttgaatga agaacaaatg tcatcgctgc tttatgaaag agatgattta agcgccattg
     1381 atttttcatt gctcatgaag acgataaaag ccttttcatt cgggggagat ctcctaaccc
     1441 ttgcatcaaa accgggttcc acaatctcaa gcatcccatc agaaagacgg atattgatta
     1501 acattaacca cgattttcct aacaatggca atcttttcaa tgactttcta tttaaccatc
     1561 aacaagatga acaattagca atggcatata tggccgctct cccgttcagc aggcctttag
     1621 tttactggga tggccaggta ttaaaatcaa cgactgaaat taaaaattat gatgggccca
     1681 cgcgtgtcgg tggtgaggcg tggcttaata aaggttgctc tacctatcag cagctctaca
     1741 atgaattcca cgcattatat atagataaag caggaatatg gagcgcattt gagggtgtat
     1801 ttgcaactaa aaacgttctg gcctttagtc gtggggattc tgtgaacatt aatcactctc
     1861 ctcatgatgg actagttata ataaataaag gaaacgaaga agttgaaggt acctggccta
     1921 acaaattgca acctggaata tata
//
LOCUS       DAZPCS010000322         1940 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZPCS010000322 DAZPCS010000000
VERSION     DAZPCS010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.328"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            94..330
                     /locus_tag="ACHUQ7_004148"
     CDS             94..330
                     /locus_tag="ACHUQ7_004148"
                     /inference="COORDINATES: protein motif:HMM:NF025073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1137281.1"
                     /translation="MSNDWHRADVIAALKKTGTPLAKLSRRSGYSTGTLANALVRPWP
                     KGERIIADALNMKPEEIWPSRYLRDHNERVDISN"
     gene            305..1936
                     /locus_tag="ACHUQ7_004149"
                     /pseudo
     CDS             305..1936
                     /locus_tag="ACHUQ7_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683974.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
BASE COUNT          528 a          418 c          531 g          463 t
ORIGIN      
        1 tggtttattt ggtaatgtat aaaacagata atcaagacga attatttgtt ttggtaaccc
       61 tttatcatac gcaatatggt tggtgtgcga aagatgagta acgattggca tcgggccgac
      121 gtgattgcgg ccctcaaaaa aacaggaacc cctctggcaa aattgtccag acgttcaggt
      181 tattcaaccg ggacgcttgc aaatgcatta gtcaggccat ggcctaaagg tgaacgcatt
      241 attgctgacg ccttaaacat gaaacctgag gaaatttggc ctagtagata tctaagggat
      301 cacaatgaga gagtggatat cagcaattga atgcatgggt ttatctggtt tacctggttc
      361 attaatgggg gttcacaaaa gagctcagag agaaggatgg ctcaagagaa gaagagaagg
      421 aattagaggg gttgcttacg aataccatat atcttctctt cctaacattg ctcgagctga
      481 agttttgctc caacaaggaa aatttaaaac cagtcaggga tgttttgaaa tcgcccgccc
      541 cacgctggaa gctcatgatt atgatcgtga ggcactatgg agcaaatggg ataaagccag
      601 cgattcccag cgcagcctgg ctgaaaaatg gttgccctca attcaggcca cagacgaaat
      661 gctgaaccag gggatttcaa cgaaaacggc ttttgcgacc gttgcagggc attaccaggt
      721 cagcgcatcc actttgcggg acaagtatta ccaggtacag aagtttgcga agcctgactg
      781 ggcggctgca cttgttgatg gacgtggagc atcccgtcgc aatgttcaca aaagtgaatt
      841 tgacgaggat gcctggcagt ttctgattgc agattatctg cgaccggaaa aacccgcctt
      901 ccgcaaatgt tatgagcgtc tggaactggc agcccgcgag catggctgga gtattccctc
      961 ccgtgccacg gcctttcgcc ggattcagca actggacgag gcaatggttg ttgcctgtcg
     1021 tgaaggtgaa catgcactga tgcatctgat accggcacag cagcgaactg tggaacacct
     1081 ggacgccatg cagtggatca acggcgacgg ttatctgcat aacgtctttg tacgctggtt
     1141 taacggtgat gtgatccgtc cgaaaacatg gttctggcag gatgtgaaaa cccgaaaaat
     1201 tctgggctgg cgctgcgatg tgagcgagaa cattgattca attcgcctct cgttcatgga
     1261 tgttgtgact cgctacagta tcccggagga ttttcacatc accattgata acacccgtgg
     1321 tgctgcgaat aaatggctga cgggaggcgc gcccaatcgc taccgcttta aggtaaaaga
     1381 ggacgatcca aaaggactgt ttttactgat gggtgcgaaa atgcactgga caagcgttgt
     1441 tgccggtaaa ggctggggcc aggcaaaacc tgttgaacgt gctttcggtg ttggtgggct
     1501 tgaggaatac gttgataagc atccggcact ggctggcgca tatacggggc caaatccgca
     1561 ggcaaaacct gataactatg gcgaccacgc tgttgatgca gagctgtttc tgaaaacctt
     1621 tgccgaaggt gtggcgatgt tcaatgccag aacaggccgt gaaacagaaa tgtgcggggg
     1681 caaactctcg tttgatgatg ttttcgagcg tgaatacgcc agaacgattg tgcgtaagcc
     1741 aaccgaagaa caaaaacgga tgctgttact gcctgccgag gcggtgaacg tttcacgcaa
     1801 aggcgagttt acgcttaaag ttggcggctc ccttaaaggc gcgaaaaacg tttattacaa
     1861 catggcatta atgaatgccg gagtgaaaaa agttgtggtc agatttgatc cacagcagtt
     1921 acacagcacg gtttattgag
//
LOCUS       DAZPCS010000323         1914 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZPCS010000323 DAZPCS010000000
VERSION     DAZPCS010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.329"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..203
                     /locus_tag="ACHUQ7_004150"
     CDS             <1..203
                     /locus_tag="ACHUQ7_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1137282.1"
                     /translation="GASYRYSGAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSIAI
                     YLLAGCLISAMTALLMKDNQRS"
     gene            305..1759
                     /gene="amn"
                     /locus_tag="ACHUQ7_004151"
     CDS             305..1759
                     /gene="amn"
                     /locus_tag="ACHUQ7_004151"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFR1137283.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITIGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSADLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDIWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT          469 a          474 c          448 g          523 t
ORIGIN      
        1 ttggtgccag ttatcgctac agtggtgctg gagtcggtta tcaggttgcc agtgtggttg
       61 gcggtggatt tacacctttt attgccgctg cactcatcac ttactttgcc gggaactggc
      121 atagcatcgc catttatttg ctggctggat gcctgatttc tgcaatgacc gctttattga
      181 tgaaagacaa tcaacgctcg tgatagcctg gcgaagatca tccgatcttc gccttatact
      241 tttgtttcac attcctgtga catactatcg gatgtgcggt aattgtatgg aacaggagac
      301 acacatgaat aataagggct ccggtctgac cccagctcag gcactggata aactcgacgc
      361 gctgtatgag caatctgtag ttgctttacg caacgccatt ggcaaatata ttacaattgg
      421 cgaattacct gatgaaaacg cccgtaaaca aggtcttttt gtctatccat cactgaccgt
      481 aacctgggac ggtagcacaa caaatccccc caaaacgcgc gcatttggtc gttttactca
      541 cgcaggcagc tacaccacca cgattactcg ccctactctc tttcgttcgt atctgaatga
      601 gcaactaacg ttgctttatc aggattatgg tgcgcatatc tctgtgcaac cctcgcaaca
      661 tgaaatccct tatccttatg tcatcgatgg ctctgaattg acacttgatc gctcaatgag
      721 cgctgattta actcgctact tcccgacaac agaactggcg caaatcggcg atgaaactgc
      781 agacggcatt tatcatccaa cggaattctc cccgctatcg cattttgatg cgcgccgcgt
      841 cgatttttcc ctcgcacggt tgcgccatta taccggtacg ccagtagaac attttcaacc
      901 gttcgtcttg tttaccaact acacacgtta tgtggatgaa ttcgttcgtt ggggatgcag
      961 ccagatcctc gatcctgata gtccctacat tgccctttct tgtgctggcg ggaactggat
     1021 cactgccgaa accgaagcgc cagaagaagc catttccgac cttgcatgga aaaaacacca
     1081 gatgccagca tggcatttaa ttaccgctga tggtcagggt attactctgg tgaatattgg
     1141 cgtgggaccg tccaatgcta aaaccatctg cgatcatctg gcagtgctac gcccggatat
     1201 ctggttgatg attggtcact gtggcggatt acgtgaaagc caggccattg gagattatgt
     1261 ccttgcacac gcttatttac gcgatgacca cgttcttgat gcggttctgc cgcccgatat
     1321 acctattccg agcattgctg aagtgcaacg tgcgctttat gacgctacca agttagtcag
     1381 cggcaggcct ggtgaggaag tcaaacagcg gctgcgtaca ggtactgtgg taaccacaga
     1441 tgacaggaac tgggagttac gttactcagc ttctgcactt cgttttaact taagccgggc
     1501 cgtagcaatt gatatggaaa gtgcaaccat tgccgcgcaa ggatatcgtt tccgcgtgcc
     1561 atacgggaca ctactgtgtg tttcagataa accgttacat ggtgagatta agcttcccgg
     1621 ccaggctaac cgtttttatg aaggcgctat ttccgaacac ctgcaaattg gcattcgggc
     1681 gatcgatttg ctgcgcgcag aaggcgaccg actgcattca cgtaaattac gaacctttaa
     1741 tgagccgccg ttccgataat aagttatgaa ccttcttatc agaaatagat gtgaaggatt
     1801 aagtaagaca tctggcaaat agcctgcaat aaacgcgggc tgcttgcttc cttgggcaga
     1861 tgcgagtttt attatcgtct taatgatttc cacatattaa aagcaagtat gctt
//
LOCUS       DAZPCS010000324         1913 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZPCS010000324 DAZPCS010000000
VERSION     DAZPCS010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.330"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(53..1702)
                     /locus_tag="ACHUQ7_004152"
     CDS             complement(53..1702)
                     /locus_tag="ACHUQ7_004152"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFR1137284.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT          452 a          506 c          491 g          464 t
ORIGIN      
        1 gaagaggata attttacagc cagcaggaag ttagcggctg gctgtaaaaa tattatggtt
       61 cgccgtacaa accaattaaa cggcgtacca caccgttagt ccagccaaac ccatcctgca
      121 acggatactc gccaccgccg ccttcgcggg gaacaccatc ggcaatatgg tatttctcga
      181 tcattttgtg ctgttccaga tagaactgat tcaccgtttt cagccagctg cgcgcgattt
      241 catcacccag aaggtcatcg ccatacattt taaatccctg aattgccatc cattgtaacg
      301 gtgcccagcc gttgggttta tcccactgtt caccggtttc gtactcgctt gccaaaatcc
      361 cgccaggtgt cagtaaccgg ctgcgtacgg cgtttgccag acgatcggcc tgttcatggt
      421 tcgccatgcc gacatagagc ggcacaatgg cggcagcgga aaacagcgcc agttgttcgc
      481 gtcgccagtc gtaatcgcgg tagatgccgt tttcatcatc ccagaggtaa cggtttaccg
      541 catcgcgacg ggcactggcc ttctggcgga acagcgcttc tgtctctttc tcgcctttca
      601 gcgccgagat gttggcgatg gcgctctcca gtttaaacag gaaggcattc agatcgatgg
      661 ggatgaactg ggtggtacga atgctcgcca gacgaccagt atcacgcagc caacgggaag
      721 agtaatccca accggaggcc gctcccgcgc gtaaatcgcg gtacacctcg ttgggcgggc
      781 gaccagaatg tttcgcggtt tcaacgtcct caagccagga ttcgtcacgc ggcgtgtcgc
      841 gatcgtccca gtaacggttg agcagcgatc cgtccggcat ccgcacaaca tggcgatagg
      901 cctgattagg gatcaacgat tctgcaccgt ccatccagaa ggcatattcc attttaaggt
      961 ggtcgagata gcggcgcgca ccgcgtacac catcttcttc aaacaactcc accatcagcg
     1021 caaaaaccgg tggttgcgag cggctcaaat aataggtgcg gttgccgttg gggatgtgac
     1081 cgtagttttc gatcatccag gcgaagttat cggccatgca tttcagcaaa tcttcccgac
     1141 cactttccgc cagtcccagc atggtgaaat aggaatccca atagtaggtt tcgctaaaac
     1201 ggccgcccgg gacaatatat gactgcggta gcgccagcag agaagaccac ggaatgtgat
     1261 cctgtggttc gcgggttagc accggccaca gctggtcgat atgctctttc agggaatttt
     1321 gcgggtccga tacatactcg ctggagtaga cctccggcag ccagaagtga ttttcaacaa
     1381 acttgcgcaa gtcaaaatca cgatgacggc gcactttacg gtagcggatt aaaatatcca
     1441 gcgggtccat tttgggtgcg cagtcgggaa aggttttact gtcggggaaa attttcgccg
     1501 actgaacatg ctcgaacagt tcgagatagc gatcggcagg cgtcagcgca tcagaggcgg
     1561 gcagcccttc aatcatctcg ggttccggtt ctgcctcgat catctcatcc agttttaatt
     1621 catacgggtc cagttcatag cagagatcga cctcgatcat cagttcgtct ggattagggt
     1681 tttgaatttt ctgattgagc ataaggatcc aacctccgaa attcgtcgct aattatcggg
     1741 cttttcttta agggtagtca ttcgctccgc ctggtgggcg acaaagcgtg cggtggatca
     1801 cgtttattaa cttgtcaaaa aatataacgc actaatatca atggaattta taaaaatacg
     1861 gatttggtgt tgtttttcat tttcagaaca ttcaatcctg ctctcgccgc aat
//
LOCUS       DAZPCS010000325         1890 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZPCS010000325 DAZPCS010000000
VERSION     DAZPCS010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.331"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(161..235)
                     /locus_tag="ACHUQ7_004153"
     tRNA            complement(161..235)
                     /locus_tag="ACHUQ7_004153"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(200..202),aa:Gly,seq:tcc)
     gene            complement(239..314)
                     /locus_tag="ACHUQ7_004154"
     tRNA            complement(239..314)
                     /locus_tag="ACHUQ7_004154"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(279..281),aa:Thr,seq:tgt)
     gene            complement(317..393)
                     /locus_tag="ACHUQ7_004155"
     tRNA            complement(317..393)
                     /locus_tag="ACHUQ7_004155"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(357..359),aa:Arg,seq:tct)
     gene            complement(395..470)
                     /locus_tag="ACHUQ7_004156"
     tRNA            complement(395..470)
                     /locus_tag="ACHUQ7_004156"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(435..437),aa:Ile,seq:cat)
     gene            complement(647..1336)
                     /locus_tag="ACHUQ7_004157"
     CDS             complement(647..1336)
                     /locus_tag="ACHUQ7_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFR1137285.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGASIPLVSQVAFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQITITHWAWEEFKAHSGTRKIAEKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTGHWVVMKHIFHRLDSEALLFVMRTRSEQKA
                     AFSKQSIAKVD"
     gene            complement(1333..1692)
                     /locus_tag="ACHUQ7_004158"
     CDS             complement(1333..1692)
                     /locus_tag="ACHUQ7_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR1137286.1"
                     /translation="MRHEFILPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDETNIVRGL
                     PVPGGRLGIKITELECA"
     gene            complement(1692..1880)
                     /locus_tag="ACHUQ7_004159"
     CDS             complement(1692..1880)
                     /locus_tag="ACHUQ7_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015967627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1137287.1"
                     /translation="MPFYFRKECPLNSGYLNMVPALRELMQAKHRAQKAVDELVDCVA
                     ELETRVGKFEKLVQEVLR"
BASE COUNT          473 a          524 c          408 g          485 t
ORIGIN      
        1 ttcactacgt aacctgaccg acaatgcacc attctggtgc tgaaaaaaga gcactggaac
       61 tgcaacaaaa caacatcaca gaacaggaga tggtaaggag tcagacattc ccacacaata
      121 ttgtgtcagt gcaaataaca accttcgtct caaatctcgc tggagcgggc agcgggaatc
      181 gaacccgcat catcagcttg gaaggctgag gtaatagcca ttatacgatg cccgcatatg
      241 gtgccgacta ccggaatcga actggtgacc tactgattac aagtcagttg ctctgcctgc
      301 tgagctaagt cggcgctggc ccaccaccga ggactcgaac ctcggaccgt caacttagaa
      361 ggttgatgct ctatccggat gagctagtgg tggttggtgg cccttgctgg acttgaacca
      421 gcgacctggc gattatgagt cgctcgctct caccactgag ctaaagggcc ggaggcagaa
      481 taataaccat atgtcatcac atctgcaaac tcatctgacc accagcacgt ttaacgtcct
      541 gtgccatttt tcaggcataa aaaaaccgct cggcggcggg ttttgcttac tttaccatcg
      601 cgtacaaaat cggcaaagta tcagatttgc atgagatata tgccttttaa tctacttttg
      661 caatactttg ctttgaaaat gccgcttttt gttctgaacg tgttctcatc acaaacaata
      721 aagcctcact atccagtcgg tgaaaaatgt gtttcattac aacccagtga ccagtaaatg
      781 ttttggacca gtttttggtt gttactcccg ccagtaatgc cagctcctga tattcataac
      841 cttccccacc aaaaagttct gcttttaccg cctgcgccgc cagccagatt aattttttca
      901 ggcgttcctg tgttttctct gcaattttcc tagtgccaga atgagcctta aactcttccc
      961 acgcccagtg ggttatggtg atctgatact cccagcaaat acttccgctg taacaccaca
     1021 acaaccaggc tttatagtgc tcttcaagag acagaacagc ccgccgccac gatgatgtcg
     1081 aaaacgcaac ctgactgacc agaggaattg acgccccctt cgccagtgat tgttttcccg
     1141 ggattggtgg attatcccgc gttatcattt ttccggtcac ctcatcgcgg taccggattt
     1201 ttttacgcct gtaacgccct gtatcgaaca tggcattctc ctgccaggct tcaagctgac
     1261 cttttgtcgc cccactcaaa tcagcggtgg cgataatgag ctgctcacga acaaactgta
     1321 aatactggtt attcatgcac actccagctc tgtgattttt atccccaacc gaccaccagg
     1381 aacaggcagt ccgcgcacaa tattggtttc atcaaactgc tcgtcgtcga taagcaaccc
     1441 cgcatgtgtc agtgcatcca gtggtgcttt caggatattg tccaggtcac gacgccgctt
     1501 atccggtggc tctgcaataa tctttatcgc cagtcttccg gacaggctta atttcagtcg
     1561 ctgctgacgg acaataagcg ccacatcccg gcgataacgc tcaccggctt ttgatacaaa
     1621 atatgtgctg ccacgacgtc gccagtaagt attcaccgtc ggcggataag gtaaaataaa
     1681 ctcatggcgc atcagcgcag cacctcctgc accagctttt caaactttcc gactctggtt
     1741 tccagctctg ccacacagtc caccagctca tctactgctt tctgtgcgcg atgcttcgcc
     1801 tgcatcagtt cccgaagcgc gggtaccata ttgaggtagc ctgagtttaa cggacactcc
     1861 ttcctgaaat agaatggcat cagaaggagc
//
LOCUS       DAZPCS010000326         1877 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZPCS010000326 DAZPCS010000000
VERSION     DAZPCS010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.332"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            1..1877
                     /gene="sat"
                     /locus_tag="ACHUQ7_004160"
                     /pseudo
     CDS             1..1877
                     /gene="sat"
                     /locus_tag="ACHUQ7_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
BASE COUNT          689 a          331 c          368 g          489 t
ORIGIN      
        1 catggataac aaaatacaat gataaactgg ttagtgagtt aaaagatacc tatagtcata
       61 aaataaatct gaatggcaat aatgtaacca ttaaaaacac agatataaca ttacaccaaa
      121 acaatgcaga taccactggt actcaagaaa aaataactaa agacaaagat attgtgttca
      181 caaatggggg aagtgttctg tttaaggata atttggattt tggttgcggt ggtattatct
      241 ttgacgaagg ccatgaatat aacataaacg gtcagagatt tacatttaaa ggagcaggaa
      301 ttgatatcgg aaaagaaagc attgtaaact ggaatgcatt gtattccagt gatgatgttt
      361 tacacaaaat aggccccggt actctgaatg ttcaaaaaaa acagggggca aatataaaga
      421 taggtgaagg aaatgttatt cttaatgaag aaggaacatt taacaatata taccttgcaa
      481 gcggaaatgg taaggtaata ctaaataaag ataattccct tggcaatgat caatatgcgg
      541 ggatattttt tactaaacgt ggtggcacgc tagatttaaa tggacacaat cagactttta
      601 ctagaattgc cgccactgac gatggaacaa caataactaa ctcagataca aagaaagaag
      661 ccgttctggc aatcaataac gaagactcct acatatatca tgggaacata aatggcaata
      721 taaaactaac gcacaatatt aattctcagg ataagaaaac taatgcaaaa ttaattctgg
      781 atggtagtgt caacacaaaa aatgatgttg aagtcagtaa tgccagtctt accatgtaag
      841 gccatgcaac agagcatgca atattcagaa gcacagcgag tcattgctcc ctggtatttc
      901 tttgtggaac ggactgggtc accgttttga aagaaacaga gagttcatat aataaaaagt
      961 tcaattctga ttacaaaagt aataatcagc agacctcatt tgatcagcct gactggaaaa
     1021 ccggggtgtt taaatttgat acattacacc tgaacaatgc tgacttttca atatcacgca
     1081 atgccaatgt tgaaggaaat atatcagcaa ataaatcagc tatcacaatc ggcgataaaa
     1141 atgcttacat tgataatctt gcagggaaaa atattactaa taatggtttt gacttcaaac
     1201 aaactatcag tactaatcta tccataggag aaactaaatt tacaggtggc atcactgcac
     1261 ataacagcca aatagccata ggtgatcaag ctgtagttac acttaatggt gcaacctttc
     1321 tgaataatac tcctataagt atagataaag gagcaaaagt tatagcacaa aattccatgt
     1381 tcacaacaaa aggtattgat atctccggtg aactgactat gatgggaatc cctgaacaga
     1441 atagtaaaac tgtaacgccg ggtctccact acgctgctga tggattcagg ctgagtggtg
     1501 gaaatgcaaa tttcattgcc agaaatatgg catctgtcac cggaaatatt tatgctgatg
     1561 atgcagcaac cattactctg ggacagcctg aaactgaaac accgactata tcgtctgctt
     1621 atcaggcatg ggcagagact cttttgtatg gctttgatac cgcctatcga ggcgcaataa
     1681 cagcccccaa agctacagtt agcatgaata atgcgatctg gcatctaaat agccagtcat
     1741 caattaatcg tctagaaaca aaagacagta tggtgcgttt tactggtgac aatgggaagt
     1801 ttacaaccct tacagtggac aaccttacta tagatgacag tgcatttgtg ctgcgtgcaa
     1861 atctggccca agcagat
//
LOCUS       DAZPCS010000327         1861 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZPCS010000327 DAZPCS010000000
VERSION     DAZPCS010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.333"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..1245)
                     /gene="mxiA"
                     /locus_tag="ACHUQ7_004161"
                     /gene_synonym="virH"
     CDS             complement(<1..1245)
                     /gene="mxiA"
                     /locus_tag="ACHUQ7_004161"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein MxiA"
                     /protein_id="HFR1137288.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKINANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLL"
     gene            complement(1258..>1861)
                     /gene="mxiC"
                     /locus_tag="ACHUQ7_004162"
     CDS             complement(1258..>1861)
                     /gene="mxiC"
                     /locus_tag="ACHUQ7_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HFR1137289.1"
                     /translation="SVNAGINSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQY
                     IEWLGNFGFNHRHTIVNFVEQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVI
                     FMKKLESSSLLKDGSLSAEQLLLTLLYIFQYPSESEQILTSVIEVSRASHEDSVVYQT
                     YLSSVNESPHDIFKSESEREIAINILRELVTSAYKKELSR"
BASE COUNT          663 a          384 c          262 g          552 t
ORIGIN      
        1 taacaaaaca atatcatcga ccttaagctc attgcttgtt ctatataaaa tagttggaag
       61 cctgacacca taatcaataa agaactgact tcttatcctt tcaataagac cttccatatc
      121 atttgcattt atcttatttt cggcaaataa taaaattaaa ggaacggttt ctgaactaat
      181 agcatccaga ttttctatca ttgccaaaga tgagtcatgg ctattatcaa tatcaaatgt
      241 accagtatag ccactagaat cagactcaga cagacttttt tctttttcta cgaccttttt
      301 ataatagaat aaagccgtca aagtaactgc tatcaggaaa aaaacaaaaa acggaaaacc
      361 tggcaacatc cctatcgcca aggcaagagc tgatgtaaca atgagaacaa aaggattccc
      421 aaatatctgg gacattatgt tacgtcccat attatcgcta tcaccattaa ctcttgtaac
      481 gataaatcct gcacttatag aaattaacag agcaggaatc tgagagacca atccgtcacc
      541 aatagttagt atggtataag tagataatgc accagatagg gacattccat gttgactcat
      601 accaacagaa atacctccta ttaaattgac aaaaataata atgatgccag caattgcatc
      661 acctttaatg aacttcattg ctccatcaaa tgaaccatac aattgactct cacgttccag
      721 aatactgcgt ctttctttag ctcctgcggc atcaataatt ccagccttta aatccgcatc
      781 aatgctcatt tgtttccccg gcataccgtc aagagaaaag cgagcagcta cctccgcaac
      841 cctttcagat ccttttgtta taacaatgaa ctgcacaaca gttactattg aaaaaataac
      901 aaaacctaca gccagactat caccaatgac aaactggcca aatgtagtaa taattttacc
      961 cgcatccgca tcaactaaaa ttagtcgact agtacttatt gataaagcga gacgaaataa
     1021 tgtcgttatc aacaaaaccg aagggaatgt agaaaaactc aatattcttt caatataaaa
     1081 tgatcccata aaaacgagaa tagcaagtac aatattaagc cctattaaaa aatcaacaag
     1141 gtatgtaggt aatggtatga tcaacatagc aatgatcata accataagca ctaatataat
     1201 taattcaggc ttagtactta cttgcttaag aaaagactgg atcactttta tctcctgtta
     1261 tctagaaagc tctttcttgt atgcacttgt gacaagctct cgtagaatat tgatcgcaat
     1321 ttctctttca ctttcacttt taaatatatc atgaggactt tcattaacag aagatagata
     1381 tgtttgatac actacagaat cctcatgact ggctcgtgat acttctataa cagaagtaag
     1441 aatttgctca ctttcacttg gatattgaaa aatatataat aaagttagca atagctgctc
     1501 tgcacttaaa ctgccatctt ttagcaagct tgaggattcc agtttcttca taaaaattac
     1561 gtctgaagtt ctaatcatct taattgcaat taatttagat aacacaaaac caaactcata
     1621 agcattacag ctcggtttct cagaatccat gtctacaatc agtgactgct ctacaaaatt
     1681 cacaattgta tgtctgtgat taaaaccaaa attaccaagc cattctatat actgatctgt
     1741 tgttgatatg ttccccatga taaaaccacg ataacatgca cgcaaaagct gtggttcaag
     1801 tttcattttg ttgccaaata atttagcctg tatagctgaa tttattccag cattaacact
     1861 t
//
LOCUS       DAZPCS010000328         1853 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZPCS010000328 DAZPCS010000000
VERSION     DAZPCS010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1853
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.334"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            313..1503
                     /locus_tag="ACHUQ7_004163"
     CDS             313..1503
                     /locus_tag="ACHUQ7_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl9 family transposase"
                     /protein_id="HFR1137290.1"
                     /translation="MPARKVCQTFFRNALASTHQYRQNAIIDSAAALVGGASLSLTSI
                     GRHLPGPARVKDKIKRVDCLLGNKRLHNDIPLIFKNITSMMTNKLSWCVIAVDWSGYP
                     FQEYHVLRASLLCDGRSIPLMSQVFPSKKNNEAVEIAFLDALSGAISPRTRVVIVTDA
                     GFQSAWFRHIKSQGWDFIGRIRGVVKFRLDSDKDKWLDIKMCRGSSEAKYLGTGTLAR
                     KKRSQCEGHFYLYKHSPKGRKSRRARGRPGLPTTEKEQKAAGREPWLIFSNTAEFNAK
                     KIMKLYSRRMQIEQNFRDEKSERFGFGLRASRSRRGERFLVLSLLVTLASIVLWLLGY
                     HFENKGFHLKYQANSLKKRRVLSFLTLAENVLRFNPELLRRAQPEKILCRLASTYRSM
                     VLAY"
BASE COUNT          503 a          387 c          436 g          527 t
ORIGIN      
        1 ccgcagtacc accgatagta tcgatatcaa atcagcctgc ggccgcgata actttcgtaa
       61 cattctgcca gcggctttgt tgaataaatc aggttcaaga taatcgtctc tgcatcaggt
      121 tagagtttca ggcaatacat cctctttctg gcatacctgc cactctcgca ccatggtcat
      181 agcctttacg atctgcatac tcaccagacc agagcccgct cccttttagg ctgaggaatc
      241 cccagaaaag aagacaggca tagagttttg tgatctactg attgcgctaa caaattcaat
      301 gagatcacct ctatgcctgc ccgtaaagta tgccagactt ttttccgtaa tgctttagct
      361 tccacgcatc agtatcgaca aaatgccatt attgattctg ctgctgcttt agtcggtggc
      421 gcatctcttt ctcttaccag cattggccgg catttacccg gtcctgctcg cgtgaaagat
      481 aaaataaaac gcgttgactg tctgttgggt aataaacgac ttcataatga tatccctctg
      541 atattcaaaa atattacatc catgatgaca aataaactgt cgtggtgtgt tatcgctgtt
      601 gactggagcg gttacccttt tcaggaatat catgtccttc gtgccagcct cctctgcgac
      661 ggtcgctcta tccccctgat gagtcaggtc ttcccgtcaa aaaaaaataa cgaggcggtg
      721 gaaatcgctt ttctggatgc gctttccggg gccatttctc cccggactcg tgttgttatt
      781 gttactgatg ctggttttca aagtgcatgg ttccgtcata tcaaatcaca gggatgggat
      841 tttattggtc gtatcagggg ggtagtgaag ttcaggctgg atagtgacaa agataagtgg
      901 ctggacataa aaatgtgcag ggggagttca gaggcaaaat acctggggac gggaaccctg
      961 gcccggaaaa aacgctcgca gtgtgaagga catttttatc tttataaaca ttcacctaaa
     1021 ggcagaaaaa gcaggcgtgc cagagggagg ccgggcttac caacaacgga aaaagaacaa
     1081 aaagccgcag gcagagaacc atggttaata ttcagcaata cagctgagtt taatgccaaa
     1141 aaaatcatga aactgtacag tcgaagaatg cagatagagc aaaactttcg ggatgaaaaa
     1201 agtgaacgct ttggttttgg ccttcgggcg agcagaagtc ggaggggaga gcgttttctg
     1261 gtattaagtc tactggttac actggccagt atcgtactct ggctacttgg ttatcatttt
     1321 gaaaataaag gatttcattt aaagtatcag gctaatagtc tcaaaaaacg aagagtattg
     1381 tcttttctga cgctggctga aaacgtcctg agatttaacc ctgaacttct acgacgagca
     1441 caacctgaaa agatattgtg ccggttggcc agcacttacc gaagtatggt gctggcttac
     1501 tgatgaaaat tttctgggga tccctcagcc tttcggggag gaatgagggc tctgatattc
     1561 ttttgccgta gttcatcgtg aaaccgtcgg atgtcatttg gcctgagtga cccatgcctc
     1621 ccaaaagcag agtcgctagt gttatgtaat taggcggtta aacttcaatt ctcagtgtta
     1681 agtttgggta aacgcttgta aatggtattg ctaatttttt aattgactaa tattagaaac
     1741 ggttagtgtg attgtatata gatatttgag tgtaaggtat ttaaaggggc attcagcccc
     1801 tccgtctatt tgtgattgca tccattgatg tgtgtattat tttgagcttg aaa
//
LOCUS       DAZPCS010000329         1851 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZPCS010000329 DAZPCS010000000
VERSION     DAZPCS010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.335"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(1..259)
                     /gene="ydfV"
                     /locus_tag="ACHUQ7_004164"
                     /pseudo
     CDS             complement(1..259)
                     /gene="ydfV"
                     /locus_tag="ACHUQ7_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfV"
     gene            284..523
                     /gene="relB"
                     /locus_tag="ACHUQ7_004165"
     CDS             284..523
                     /gene="relB"
                     /locus_tag="ACHUQ7_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HFR1137291.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            523..810
                     /gene="relE"
                     /locus_tag="ACHUQ7_004166"
     CDS             523..810
                     /gene="relE"
                     /locus_tag="ACHUQ7_004166"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HFR1137292.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
     gene            882..1037
                     /gene="hokD"
                     /locus_tag="ACHUQ7_004167"
     CDS             882..1037
                     /gene="hokD"
                     /locus_tag="ACHUQ7_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR1137293.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1255..1506
                     /gene="rem"
                     /locus_tag="ACHUQ7_004168"
     CDS             1255..1506
                     /gene="rem"
                     /locus_tag="ACHUQ7_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein Rem"
                     /protein_id="HFR1137294.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNIVSHYRIVCLQKNGAALIN
                     FVDARVTDGFILRDGEFVTSLQALKEIGIKAGFSAFSEE"
     gene            1573..1851
                     /locus_tag="ACHUQ7_004169"
                     /pseudo
     CDS             1573..1851
                     /locus_tag="ACHUQ7_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          509 a          410 c          467 g          465 t
ORIGIN      
        1 gacatgctaa agcaaaaaat agatgaataa gttgagttgt gcatatgtag cctgaccgtc
       61 acaaagtata tggtgtctgt accagtaaga tgatggccgg actctttaaa aaaaagagct
      121 gacctgcaca atacaggatg gacttagcaa tggctgctcc tggcacaaag cggacagtga
      181 tcaccgttct tacgactact ttctgacttc cttcgtgact tgccctaagc atgttgtagt
      241 gcgatacttg taatgacatt tgtaattaca agaggtgtaa gacatgggta gcattaacct
      301 gcgtattgac gatgaactta aagcgcgttc ttacgccgcg cttgaaaaaa tgggtgtaac
      361 tccttctgaa gcgcttcgtc tcatgctcga gtatatcgct gacaatgaac gcttgccgtt
      421 caaacagaca ctcctgagtg atgaagatgc tgaacttgtg gagatagtga aagaacggct
      481 tcgtaatcct aagccagtac gtgtgacgct ggatgaactc tgatggcgta ttttctggat
      541 tttgacgagc gggcactaaa ggaatggcga aagctgggct cgacggtacg tgaacagttg
      601 aaaaagaagc tggttgaagt acttgagtca ccccggattg aagcaaacaa gctccgtggt
      661 atgcctgatt gttacaagat taagctccgg tcttcaggct atcgccttgt ataccaggtt
      721 atagacgaga aagttgtcgt tttcgtgatt tctgttggga aaagagaacg ctcggaagta
      781 tatagcgagg cggtcaaacg cattctctga accaaagcat gacatctctg tttcgcaccg
      841 aaggtgacac ttctgctttg cgttgacagg agaagcaggc tatgaagcag caaaaggcga
      901 tgttaatcgc cctgatcgtc atctgtttaa ccgtcatagt gacggcactg gtaacgagga
      961 aagacctctg cgaggtacga atccgaaccg gccagacgga ggtcgctgtc ttcacagctt
     1021 acgaacctga ggagtaagag acccggcgag tgagaaatcc ctcgccacct ctgatgtggc
     1081 aggcatcctc aacgcacccg cacttaaccc gcttcggcgg ttttttgttt ttattttcaa
     1141 cgcatttgaa gttctggatg gtgccggaat agaatcaaaa atacttaagt agcgcgcagg
     1201 gataaaaggg atggaccccg aacaggggag tgctacttat ctggaaggat tctgatgatg
     1261 aacatcgaag aactgcgtaa aattttttgt gaagatggcc tctatgctgt gtgcgttgaa
     1321 aatggaaata ttgttagtca ttaccgcatt gtgtgtttgc aaaaaaatgg ggctgcgtta
     1381 attaattttg tggatgcccg agtgacggac ggatttatct tgcgcgacgg tgagtttgtc
     1441 acttcattac aggcattgaa agagatcgga ataaaagctg gcttttctgc tttttcagaa
     1501 gaataaactc atctacaatc ttgcgcgggg ctgaactccc gctgagtaac accgtgccac
     1561 cggagaaaac cgatggcacg caacgcaaaa tattacaatc atgataattc gaccgttctt
     1621 gcccacacgc acgagcggta ttctcacgca tttaagtcag actggtacca gcatccctca
     1681 tgcactgaag aacaggccga atggcttatt cagtgttacc gcaggcgcgg atgcgaggtt
     1741 aaaaaagccc ttagcctcga ctaccgtcac tagataatct ccgtcaggct cccttactcc
     1801 gaacagccac cgcgtccgtc ccgcacattc cagcaacgga tctggaggta a
//
LOCUS       DAZPCS010000330         1847 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZPCS010000330 DAZPCS010000000
VERSION     DAZPCS010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.336"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(1..355)
                     /locus_tag="ACHUQ7_004170"
                     /pseudo
     CDS             complement(1..355)
                     /locus_tag="ACHUQ7_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(355..1275)
                     /locus_tag="ACHUQ7_004171"
     CDS             complement(355..1275)
                     /locus_tag="ACHUQ7_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR1137295.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPHIEAGSNARTSDISSEEYAIMPLAPMKEADAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1413..>1847
                     /gene="pgrR"
                     /locus_tag="ACHUQ7_004172"
     CDS             1413..>1847
                     /gene="pgrR"
                     /locus_tag="ACHUQ7_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFR1137296.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMVQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPILHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGV"
BASE COUNT          461 a          454 c          426 g          506 t
ORIGIN      
        1 ggcacccccc acacacaccc agcacgccaa tgcgttcagc atcaacatat ggcagagtta
       61 ctgcataatc gataacacgg ctaatatcct ctacgcgctg tgtcggatct tctatccaac
      121 gaggctaccc accagattct ccctggaaac tggcgtcata agcgagaacg acatagcctt
      181 tttcagccaa tgccttgcca tagatattgc cagacgtctg ctccttacaa ctgccaaaag
      241 gatggacgct gataatcgcc ggatactgtt ttgactcatc aaaatttggt ggaaactgaa
      301 tattggcagc aatatgccag gccatatcac tattgcggat ttgaatatgt ttcattacag
      361 agtttcctca aagaacggag ctaatactga aattgcttca gccacatagg ctttgccgtc
      421 ataaagatcc atatggtttg cgccttcaac aatgtgatag cgtttatcct ggcttgatgc
      481 ccgatcgtac aggtcgtcac tcatccattt gctccctgcc tggctgcccg ccacaatctg
      541 catcggctga gtcaggtaca cttccgccat atggtaagca tcataggtaa taatctggtt
      601 aaggctgcgc aaagtagcgt aacccggtgc tgttggatac tgcgcacgag gtgtgtggta
      661 atactcccag gcctgacgca gttcttcgtt cggcgcatcg gcctccttca ttggagccag
      721 tggcataatg gcgtattctt cgctgctaat atcgctggtt cgggcgtttg agccagcttc
      781 aatgtgtggc agagcatcaa tagatttcac attgttttcc caaccattgc ggaacatcga
      841 accaatattg accgcactaa cggtaccgat ggccttgatg cggcgatcct gaattgcagc
      901 attggctgta tatcctgcac cggcacaaat tcccatcgca ccaattcggg tattgtcgac
      961 ataagaaagc gttgtcaggt aatcaatcac ggcactgacg tcttcagtac gaatgtatgg
     1021 gttttctaac tgacgcggct cgccgccact ttcaccttga taagatgcgt cataagcaat
     1081 agtgacaaat cccttttccg ccagcttttc ggcataggtt ccggccgttt gctctttaac
     1141 gcccccacct gggtgagata ccacaattgc cggatactga cgggtttcat caaattttgg
     1201 agggaaatag atcactgcag acaaagagat ggttggatta ttgctgttag tgaagctgac
     1261 tttattattc atcattcagt tcctcatgga gttgtcggtt cgttttgacg gttggtgata
     1321 tcactataga tattgatcat taagttgatt agaccctaaa tcatgattag acctatcatt
     1381 taaatgatta atagattgta tggagatact gaatgaagcg tgaagaaatc gctgatctga
     1441 tggcgtttgt cgtcgttgca gaggagcgta gcttcactcg cgcagcagcc cgcctgagca
     1501 tggtgcagtc agctttaagc cagatagtgc gccgtataga agaacgattg ggattgcggc
     1561 ttctgacgcg aaccacgcgc agcgttgtac ccactgaagc gggcgagcat cttttgtctg
     1621 ttcttggccc tatattgcat gacatagatt ctgccctgac atccctgagc gatctgcaga
     1681 accgcccatc cgggacaata cgtattacta ctgtagaaca tgcagcaaaa acgatattgt
     1741 taccagccat gcgcacattc ctgaaatcgc atcctgaaat tgatattcag cttaccattg
     1801 attatggttt gaccgatgtc gtttctgaac gttttgatgc aggcgtc
//
LOCUS       DAZPCS010000331         1834 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZPCS010000331 DAZPCS010000000
VERSION     DAZPCS010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.337"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..182)
                     /locus_tag="ACHUQ7_004173"
     CDS             complement(<1..182)
                     /locus_tag="ACHUQ7_004173"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1137297.1"
                     /translation="MAGCVGEPKGSPVSVPGYANPAQFATSSIGVVGGDNFQMYGVIV
                     MTTQISVETLSPITHN"
     gene            complement(323..877)
                     /locus_tag="ACHUQ7_004174"
     CDS             complement(323..877)
                     /locus_tag="ACHUQ7_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFR1137298.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLASKKLTKTAVNEALRRMKKAGIEKPELEAFLREMINGKQKTWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPEWCFRTCCSRVDVWL
                     NLAEYMLYLPMRDAFSSGDLKTVC"
     gene            complement(874..1164)
                     /locus_tag="ACHUQ7_004175"
     CDS             complement(874..1164)
                     /locus_tag="ACHUQ7_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFR1137299.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHNPETSVLAHYRLAGTCGTATK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(1164..1763)
                     /locus_tag="ACHUQ7_004176"
     CDS             complement(1164..1763)
                     /locus_tag="ACHUQ7_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFR1137300.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSDAAEQ
                     YLNTVATCRTRDTALLKSFDAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEEVENVAAQLLEFA"
BASE COUNT          445 a          496 c          422 g          471 t
ORIGIN      
        1 tggttatggg tgatcgggga gagagtttca acagagattt gagtggtcat aacgataact
       61 ccgtacattt ggaaattatc gccaccgacg acgccaatcg aactggtggc gaactgtgca
      121 gggttggcgt aaccgggtac ggaaaccggc gagcctttcg gctcccccac acagcccgcc
      181 ataaatcgcg aatgtgactg tgcaaacgat atgaaaaaag acgcgggcgc gtctcatatc
      241 gctccgtaaa catccgggac gccaatcccg acgccagatt ttgctggcgc gtgaggaata
      301 tagccccgga taacagattg agtcaacaaa cggtttttag atccccggaa gagaatgcat
      361 cacgcatcgg tagatagagc atatattcag caagatttag ccatacatct acccgactgc
      421 agcacgtccg gaaacaccac tcagggtgtg cgtcattcag caattcagcc attttacgct
      481 tagtcatccc ccgcccttca taccgttgcc ggaggacact aatcaatcca ggatgctctg
      541 ccagcacctc acttatgact ctatcaatac ataacgcctc tgcatcagta caatgcgcca
      601 gccaggtctt ttgcttgcca ttgatcatct ctcgcaaaaa cgcttccagc tcaggtttct
      661 ctattcccgc ttttttcatc ctgcgcaggg cttcattgac ggctgttttc gtcaattttt
      721 tggatgccag caactggtta aacatatttc ccgtcttacc gccgccaata tacgaccagc
      781 gcccccacat gcgcagtttt ccctgaatcc agacactttc cagcgtggtg agacgaaggt
      841 gttccccgct tttgcctgta tttgttgggt aaatcataaa taaccttcct ttctccagat
      901 ttcttgtgtg cgaaaaacac cttctgcatg catcaggcgt aattcttctt tggtgtaatc
      961 gctggttttt acccgcccgt cgattaaatc gtggcatgag ctacaggcaa tcgctgcctg
     1021 catatcgtgt ggttttgtcg ctgttccgca cgtccccgcc agcctgtaat gcgccagcac
     1081 agaagtttcg ggattgtgat tgcagtagcc aggaattctg acggtgcaca tctgcccccg
     1141 cgccgcttta cgtaaatcca ccattacgca aactccagta actgcgcggc cacattttcg
     1201 acttcctccg gagaggaaaa tttacggaac aggatccagt tccacagcac attcagtaca
     1261 gatttataaa cctgctgaaa ctcggtttcg tccatgttcg caaatgcgat agattttgcc
     1321 ctgcgcccac gactaccatc aggataaata tgctcggtgt aaaatccggc ctgaatggtt
     1381 acccactcgc ggaaagcgtc aaacgacttt agcaatgccg tatcccgggt tctgcatgtc
     1441 gcaactgtat taaggtattg ctctgcggca tcactcaggg ctggagtgtg ttcccgacct
     1501 actgattcgc acaggtaatc aacgaagcct gataccagtt ttcgttcgcg aggcgtgatc
     1561 gccccaccga ccggagtcca gtaatcaaat cccagttgca ggagtttgaa aaaacgcttg
     1621 tggaatgcgt agttacgcac acgcttaaag tctgcgtgta tccactcacc tattttgatt
     1681 tgatgcaaaa aatcgcaact ctccggcgtc gccgggagaa gtaatccgga agaggtttgt
     1741 ttgaccagtt gtatatgcgc catcgtagtt ctccgctggc gcagtagaat gggtgttcag
     1801 cccgttatgt agtataccag aattaatgcc aata
//
LOCUS       DAZPCS010000332         1823 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZPCS010000332 DAZPCS010000000
VERSION     DAZPCS010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.338"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..374)
                     /locus_tag="ACHUQ7_004177"
     CDS             complement(<1..374)
                     /locus_tag="ACHUQ7_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1137301.1"
                     /translation="MNAIDNIFKYIPSNHKDKHSDKVNNHQHHSKVDKTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPIT"
     gene            792..1790
                     /locus_tag="ACHUQ7_004178"
                     /pseudo
     CDS             792..1790
                     /locus_tag="ACHUQ7_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
BASE COUNT          487 a          362 c          366 g          608 t
ORIGIN      
        1 gtaatgggag acgataattt tatcacatgg ttattgtctg gtataattgt aaagataaac
       61 gtgttagtag tattatcggc aaaactatta tattcttgaa tatctataga tggctcacta
      121 ttgtaaaacc atattttctc catctgaaga ctatgttcat ttaccctcca gtgaccatgt
      181 tgcaggaaat agatgatatg catgccaaaa tcgttatcta tttgcgaatc attatccagc
      241 ttatcaattt caactatctt tgaacgatgt gttttatcaa ccttgctatg gtgttgatga
      301 ttattaactt tatctgaatg cttatcttta tggtttgaag ggatgtactt aaatatattg
      361 tcaattgcgt tcataaataa cccgcgatat ttttgacctg ttagtaaaaa ataaatgata
      421 catgtctgaa agacatattt acgacgatat aaaacaataa tacttatatg ttcgctgatt
      481 ttaagcaccg agactatccg gtcttttact aaataatgga aaacaatcca tccctggaag
      541 acaattgtaa aaaaaaacaa cagaataaga attaatctta ggataaatat ttatttatca
      601 tggccttttg accgctaaaa aatggcactc ggcacagtaa accccgctat agttatgcta
      661 tgcatttgtc tttaatgata tctcgtcgtt atattaacag gctgaaatta tcattaatgc
      721 acatttcaat attagcaggg atacctgagg agtttaaagt gaacatatat atcgggtagc
      781 ttttcaaatt aatccctttg attataggct taatttgcat cgcgttaggt ggctttgtgc
      841 tggaaagttc agggcaacgt cacgggtcat gtgctgattt ctctggcggc catatgcctg
      901 gcattattca ctaccgcatt tattatcatt tcgcaactca cgcgcggcgt taatacgttt
      961 tacaatacat tgttccccat tattggctat gcggggtcaa ttatcaccat gatatggggt
     1021 tgggcactgt tagcaggtaa tgatgtgatg gcagacgagt ttgttgccgg ccatgttatt
     1081 tttggcgttg gtatgattgc cgcatgtgta tcgacggtgg cagcgtcgtc cggtcacttt
     1141 ctgctcattc ccaaaaatgc agcggggagc aagagcgacg gaacaccggt acaggcttat
     1201 tcttcattaa tcggtaattg cctcattgcc gttcccgttt tactcaccct gctcggtttc
     1261 atttggtcta ttacgctgtt acatagtgcc gacataactc cgcattatgt cgccttgggt
     1321 taaccgcaat ctgtgcctgt ctaattggcc tagtggccac aatcgtccat caaacacgta
     1381 atacgttctc atccaaagag cactggctgt ggtgttattg ggttattttt ctcggctcag
     1441 tcacggtgct gcaggggata tacgtcttag tcagttccga tgcaagcgcc cgactggctc
     1501 ccggcattat tctcatttgc ctcggaatga tctgttacag catattctca aaagtctggc
     1561 tactggcact ggtctggaga cgtacctgtt cgttagccaa cagaataccg atgataccgg
     1621 tatttacctg tctgttttgc ttgtttttgt catcattcct cgcggaaatc gcacaaactg
     1681 agctggacta ctttattcca tctcgggttc tggttggatt aggcgcagta tgttttacat
     1741 tgttctctat tgtttcaata ttagaggccg gttctgcgaa aaaaaattaa aatttacata
     1801 atcccagcgt tattcatatc tta
//
LOCUS       DAZPCS010000333         1821 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZPCS010000333 DAZPCS010000000
VERSION     DAZPCS010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.339"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..362)
                     /locus_tag="ACHUQ7_004179"
     CDS             complement(<1..362)
                     /locus_tag="ACHUQ7_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1137302.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGL"
     gene            complement(429..1643)
                     /gene="manD"
                     /locus_tag="ACHUQ7_004180"
     CDS             complement(429..1643)
                     /gene="manD"
                     /locus_tag="ACHUQ7_004180"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-mannonate dehydratase ManD"
                     /protein_id="HFR1137303.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIGYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPYSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPEYLP
                     VARLEDGTLLNW"
BASE COUNT          544 a          472 c          375 g          430 t
ORIGIN      
        1 agaccgatac gcattaaaat aaagtggcta aaagaattaa ccattcctgt taatgcctgc
       61 attaatgacc agaatattaa gcctgcgcct aaaactattc gtgggccaaa gcggtctagc
      121 aaaataccgg aaggtagttg tgaaaaacca taagacagag aaaatgcaga aagcaaaaca
      181 ccaaactctg tcgccgataa tcctaactcg ccacgaatcg cttcgcctgc cacgctcaat
      241 gacgaacggt cgagaaaatt aacgatcccc gccataaata ataaaaccag ggtcactgtc
      301 tgaatacgac gaatccgttg cggtttatta cccgcaacac tggttgacac atcaacgtcc
      361 atttttagct ccttgagcgg aattaatcga tttgatgact attcagaaaa taattaagcg
      421 aaccgttatt accagttcaa caatgtgcca tcttccagtc gtgcgacggg gagatactcc
      481 gggtcatacg gatatttcgc cgccagtttc tcatcgaaag aaatacccag ccccggttca
      541 tcgcctgggt gcatataacc ttcctcgaag cgccagctgt acgggaaaac ttcaagcatt
      601 tgttcggaat agcccatgta ctcctgaacg ccaaaatttg gtacccatag gtcaaaatgc
      661 agcgccgccg catgacaaac aggcgataaa tctgaagggc catgcgaacc ggtacgtacc
      721 tgataaagcg atgcaaaatc ggcaatccgc cgcatcccgg taatgccgcc tgcatgggtg
      781 atcgtcgtgc gaatatagcc aatcagctgc tcttcgatta actgcttgca gtcccagata
      841 ctgttaaaca cttcaccgac cgcaatcggc gtcacggtat gctggcgaat cagacgaaaa
      901 cattcctggt tctcggcagg cgtcggatct tccatccaga acaggcggta atcctcaatg
      961 ctcttcccaa agcgcgccgc ttcgatgggg gtcaggcgat ggtgcatgtc atgcaacaga
     1021 tgttcgttaa aaccgaactt attacgcacc gcttcgaata acttcggcgt aaaatcgaga
     1081 tacttttctg aagaccacaa ctgctcttct ggccacaggc ctttggttgc cggttcatac
     1141 gccagtcctt tgcccttcgc caggccataa gtggtttgca tacccggtac accgcactgc
     1201 acacgaatcg ccttaaagcc catctgctgg tgctttgcat agtcttcaag gacttcatca
     1261 atcgtacgcc cggtggtgtg gcaataaacc atcacgcctt cacgagaagc accaccaagc
     1321 agttgataca atggcatatt tgccgcttta gctttgatat cccacaacgc catatcaatg
     1381 gcagaaatgg ctgacatcgt gacggggcca cgtcgccagt aagcaccttt ataaaagaat
     1441 tgccagatat cttcgatacg gctggcatcg cggccaatta gttgtggaca aagatgatct
     1501 ttcagataag aagcgaccga caattcgcga ccatttaacg tggcatctcc taatccacaa
     1561 agtccgcttt cagtggttat tttaagcgta acgaaattac gccctgggca agtaacaaaa
     1621 acatctgcag caataatttt cattattgca atctcctgtg ttgtaaatag acgtttcaaa
     1681 cctaatttta cgcctactac catacaagag agaaaaagcg attattcaac cgtgatcaca
     1741 ttaaaaacaa aaatttctac cgaggagaaa aataccctca aaaaccacct gaataattaa
     1801 ccattattgt taaaacaaca c
//
LOCUS       DAZPCS010000334         1809 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZPCS010000334 DAZPCS010000000
VERSION     DAZPCS010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.340"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            173..1198
                     /locus_tag="ACHUQ7_004181"
     CDS             173..1198
                     /locus_tag="ACHUQ7_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR1137304.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPRFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVSNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            1266..1809
                     /locus_tag="ACHUQ7_004182"
                     /pseudo
     CDS             1266..1809
                     /locus_tag="ACHUQ7_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc.;
                     frameshifted; incomplete; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
BASE COUNT          421 a          421 c          462 g          505 t
ORIGIN      
        1 caccattgcg gggcgctgtt tttattattt cctgtctaca ctcagcttat tgcgacgcga
       61 aatcgtgcgc aaaagctggc actacttttg cttataagag gatggccaca gacaggtaaa
      121 agacgtttcc caaacgtcct ataacgataa ttttcgcctc acaggaagca ttatgaaaaa
      181 gatgatgata gccacactgg ctgccgccag cgtgctgctt gccgttgcaa atcaggcgca
      241 tgctggcgcg acgcttgatg ccgttcagaa aaaaggtttt gtgcaatgcg ggatcagtga
      301 tggattacct aggttctctt atgccgatgc agacggtaag ttttcaggta ttgatgttga
      361 tgtttgtcgt ggtgttgccg ctgctgtatt tggtgacgac acgaaagtga aatatacccc
      421 actcacagca aaagaacgct tcaccgcttt acagtcaggg gaagtggatt tgctctcccg
      481 taatacgacc tggacttcat ctcgcgatgc cgggatggga atggcattta ccggcgtcac
      541 ttattacgac ggcattggct tcctgacgca cgataaagcg gggctaaaaa gcgcgaaaga
      601 actggatggc gctaccgtct gtattcaggc gggtactgat accgaactca acgtcgccga
      661 ctatttcaag gcaaacaata tgaagtacac accagtgact ttcgatcgct ctgacgaatc
      721 agcgaaggca ctggaatctg gtcgctgcga tacgctggct tcggatcagt cgcaactgta
      781 tgccttgcgc atcaaattaa gcaacccggc tgaatggatt gtcttaccgg aggttatctc
      841 taaagaaccg cttgggccgg tagttcgtcg tggcgatgat gaatggttct cgattgtacg
      901 ctggacgctt ttcgccatgc taaatgctga agagatgggc atcaattccc agaacgtcga
      961 tgaaaaagcg gctaatccag caacgcctga tatggcacat ctgttgggta aagaaggcga
     1021 ttacggcaag gatctgaagt tggataataa atgggcctat aacatcatca aacaggtgag
     1081 taactactcg gaaatttttg agcgtaacgt aggttcagaa agcccgctga aaattaaacg
     1141 tgggcaaaat aatctctgga ataacggcgg tattcagtac gcgccgcccg tgcgttaagt
     1201 cggtgtgagg taacgagcgt cgctacggcg gcgctctctt cagcgttatg ctttccgagg
     1261 tttctatgcc tcatcgccgc tcaaccgtta aaggctcact ctcctttgcc aaccctacgg
     1321 ttcgtgcctg gttattccag atccttgccg ttgttgctgt tgtcggcatt gttggttggt
     1381 tatttcaaaa cactgtaacg aatctcaata atcgtggcat tacttcaggt tttgcctttc
     1441 tggatcgcgg cgctggcttc ggtattgtcc agcatttgat cgattaccag cagggcgaca
     1501 cctacggacg cgtttttatt gtcggcttac tcaatacgct actggtttct gcattgtgta
     1561 ttgtgttcgc ttctgttttg ggcttcttta tcggtctggc gagactttcg gataactggc
     1621 tgctacgaaa gctttccaca atttatattg agatcttccg taatattgca aatcttcttc
     1681 tggtactttg ccgtgttgcg caatttgccc ggaccacgcc aagctgtgag cgcgtttgat
     1741 ctggcctttt tgagcaatcg tgggctttat attccgtcac cgcagctggg agacggattt
     1801 attgcgttt
//
LOCUS       DAZPCS010000335         1768 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZPCS010000335 DAZPCS010000000
VERSION     DAZPCS010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.341"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            7..657
                     /locus_tag="ACHUQ7_004183"
     CDS             7..657
                     /locus_tag="ACHUQ7_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1137305.1"
                     /translation="MHRIDTKTAKKDKFGAGKNGFTRGNPQTGTPATDLDDDYFDMLQ
                     EELCSVVEASGASLEKGRHDQLLTALRALLLSRKNPFGDIKSDGTVKTALENLGLGEA
                     AKRNVGTGANQIPDMSLFASINTVTAAAQKFPSGLILQCGQLNGAPNVSSTYGMRFPM
                     TFSRVIAVVVTLNVTGAAGQPTVSATSVQNTGFNITVSPGSGYGSSADAYYIAMGY"
     gene            629..1063
                     /locus_tag="ACHUQ7_004184"
     CDS             629..1063
                     /locus_tag="ACHUQ7_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR1137306.1"
                     /translation="MRITLQWDINKMSYFYSASTNGFYSTEFHGTNIPDDAVEISESE
                     WKTLINAQSVTKMITCGENGHPVIVDRPSPTPEQLALINDEKKSALIAEATNVIAPLQ
                     DAVDLGMATDDETKLLLAWKKYRVLLMRVNVVKPEWPMHPNK"
BASE COUNT          496 a          350 c          407 g          515 t
ORIGIN      
        1 taatccatgc atcgtataga cacgaaaacc gcgaagaagg ataagttcgg cgcgggtaag
       61 aacggtttta cccgtggtaa cccccagact ggcacgcctg ccaccgatct ggatgatgac
      121 tactttgaca tgttgcagga ggagctttgc agcgtggtgg aggcatccgg tgccagcctg
      181 gagaaggggc ggcacgatca gttgcttacc gcacttcgcg cgctgctgtt aagccgcaag
      241 aatccgtttg gcgatatcaa atcggatggc acggtgaaaa cggctctcga aaaccttggt
      301 ttgggagaag cagctaaaag gaatgtaggt acaggggcga atcagatacc tgatatgagc
      361 ctgttcgcgt caattaatac cgtaacggct gctgcgcaaa aatttccgtc tggattaatt
      421 ttacagtgcg gtcagttgaa tggtgccccg aatgtatctt caacatacgg gatgaggttc
      481 ccgatgacgt tctcaagagt aattgctgtc gtagttacat tgaacgttac tggcgcggct
      541 gggcagccga ctgtatcggc gacaagtgtc cagaacactg gatttaatat tactgtgtcg
      601 cccggttcag gatacggttc atctgctgat gcgtattaca ttgcaatggg atattaacaa
      661 aatgtcatat ttttattctg catcgacaaa cggattttat tcgactgaat ttcacggcac
      721 caatattcct gatgatgcag tggaaatctc ggaatcagag tggaaaacac tgattaatgc
      781 acagagtgta acaaaaatga ttacctgtgg tgagaacggt catcctgtca ttgttgaccg
      841 tccttctcca acaccagaac aattagcctt aataaatgat gaaaagaaat ctgcactgat
      901 agcagaggca acgaatgtaa tagctccgct tcaggatgcg gttgatttag gtatggcaac
      961 agatgatgaa acgaaactgt tactggcatg gaaaaaatat cgggtgctgc ttatgcgtgt
     1021 taatgtagta aaacccgagt ggcctatgca tccaaataaa tagacttgtt aatctaggat
     1081 aattgttgga ataatactcg tttttcagta ggttcaggta gcctgggtat aatgctcact
     1141 tctacaatga tttcaactta ttttatttaa ttgatattac gaaacttaac tcatacataa
     1201 acaatatgac tcaatactgt cctaaatagc tgcgcagaaa agtgctccac tttcgctcag
     1261 cccttgctgg gcgaagcatc aggaaggcgt tctcagctag gttgtgttat atctggcttg
     1321 agttttgttg cccatacgca acacattacc catctgtaaa cgacaagtgt ggcagaatta
     1381 ggtgcagcta tttagactat atcgacgcag taacgataat agatatcgaa actgtactag
     1441 atattagcta cttatggtta ccggggggag tcttttgagc actggtgtta tcaattggct
     1501 tcaaataatt cgccaataaa ttatattgcg atagctcatc accttccagg ttgtttatag
     1561 ccagtttatc agcgtcacca cctactgttt atgcttttat ttctttgatt aataagtata
     1621 tgatatttta attcatctgg tgtatttctt tctcatgtgt tggtcgactg aatatctgtt
     1681 cagtgaaggg gtaattttat gtttgcactt cgaacattcc cttcagttgc taggaaagaa
     1741 aaccgtaaca tttgcgcatt aattttaa
//
LOCUS       DAZPCS010000336         1760 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZPCS010000336 DAZPCS010000000
VERSION     DAZPCS010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.342"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(1..264)
                     /locus_tag="ACHUQ7_004185"
                     /pseudo
     CDS             complement(1..264)
                     /locus_tag="ACHUQ7_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(379..1017)
                     /gene="ospI"
                     /locus_tag="ACHUQ7_004186"
     CDS             complement(379..1017)
                     /gene="ospI"
                     /locus_tag="ACHUQ7_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspI"
                     /protein_id="HFR1137307.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
     gene            complement(1164..1349)
                     /locus_tag="ACHUQ7_004187"
     CDS             complement(1164..1349)
                     /locus_tag="ACHUQ7_004187"
                     /inference="COORDINATES: protein motif:HMM:NF015365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR1137308.1"
                     /translation="MLPLSVNLMSSGASLAARLQQHWLWYSYNTKTGGVLAYTFGPRN
                     DETCRELLVLFTPFCIY"
     gene            complement(1374..1646)
                     /locus_tag="ACHUQ7_004188"
     CDS             complement(1374..1646)
                     /locus_tag="ACHUQ7_004188"
                     /inference="COORDINATES: protein motif:HMM:NF024169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element transposase"
                     /protein_id="HFR1137309.1"
                     /translation="MATVTVHCPRCHSDEVYRHGRSCSRHERFRCRSCKRVFQLTYSY
                     EARKPGVKEQIVEMAHNGAGGRDTARTLKIGINTVIRTLKSSRPGG"
BASE COUNT          502 a          434 c          319 g          505 t
ORIGIN      
        1 cagcaagcgc tgtaactctt ttatttgttt tatagcggca gccagttctg aggcaggaac
       61 gacatcctct ccggcagcaa cagcagtgag acttccttcc agatattatt ttttccactt
      121 aagcaacagg ctgggctgaa taccatgcaa acgcgccaca tgggacaccg tcatgccagg
      181 ctccattgtc tgctgaataa tggcaatttt ttcctgagga gttttacgtt tacgaagctc
      241 cagccccagc aaagttccag tcatctcaaa attagaacta gtgttagaca tatatccaag
      301 cctatctctt attttaagat acatccactg tctggcgttt caaggggccg gctcaaatct
      361 atgtacaggc tctcagcatc agcaaagcct cttacttttc caactactac tgttagcata
      421 gtgatcaaat tcctgatcag atacatgaat tacctgaacc gaactatttt catctacaaa
      481 tgcatctggc gatttttcta caggatcata aagattaaat ccctttcctg tttgaacatc
      541 caaaaacata attttttcac catcgttaaa tccagctatc caatgagtgg cctgttcaat
      601 agaaatcatt actacacttc cttttgaagt attttgtaaa atattttctg caatacgctc
      661 acgaaattta ctataagttt cattaggatg tgacactatg ccttcactat caatttgctc
      721 taaatgccta tgaaaaaatc cggtcatata ctctgatatt tcattaggta cgggagcctc
      781 tcttactcca taccctaaca tagccataca agcatgaagc gcacaccctg aacagttacc
      841 atccccacca gacggattta tcattttctg taaagtcgct gccaattcag aatctttatc
      901 catatcacca agacttttag agtttaaaga ttccagcaat tttttaacat ttacatttcc
      961 ctcctctgta tgtgcctcat atccagcagt tccctgtaac gacaccccat taatcatatt
     1021 gtatcctgac agtatatata ctcactatat tttcttcata acaaatatcc ctctgatatg
     1081 acaacaaaaa acggagccgg attcctgttt ttgtgaactg cagctattcg tgtctaaaaa
     1141 gtgatccact taccaccaac ggcctaatat atacagaacg gagtaaacag gactaacagt
     1201 tcccgacagg tctcatcatt ccgtggcccg aaagtgtaag ccaacacacc gccagtttta
     1261 gtgttgtacg aataccagag ccagtgttgt tgcagccttg ctgccaacga agcgccactg
     1321 ctcatcaagt tcacagataa gggcaacatc agcgtgagcg actggcgcag atgttatccg
     1381 ccggggcggg agctttttaa tgtccgtatg acggtattga tgccgatttt cagtgtcctg
     1441 gcggtatcac ggccccctgc gccgttatgt gccatttcaa cgatctgctc cttaacgccg
     1501 ggttttctgg cttcataaga gtaagtgagc tggaaaacac gtttacatga gcgacaacgg
     1561 aaacgctcat gccgggaaca actacggcca tgtcgataaa cttcatcgga atgacagcgc
     1621 ggacaatgaa cagtaacggt agccatggaa aaccttaaat accagtagat taccagaaac
     1681 ttctgtcgat tgaagacatc acccgaagtt tgaattcatt cgaatacttt ttacgaggtt
     1741 cttttcgcca gtcctgtaat
//
LOCUS       DAZPCS010000337         1750 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZPCS010000337 DAZPCS010000000
VERSION     DAZPCS010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.343"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            455..1303
                     /locus_tag="ACHUQ7_004189"
     CDS             455..1303
                     /locus_tag="ACHUQ7_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFR1137310.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKTGAGIATPTMTTAHNRAQAVFLC
                     VKHSHIQIMVGRAGQPQGWPVSVVTGCSNPVRLTTHEIATSGGESFKLTIEAAIMATI
                     LTLSHPDATIENGRAVTTSVAVAEFFRKMHKNVIQKIETLECSPEFNRLNFKPVTYTD
                     AKGEKRPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEAELRQNNTPPADKMI
                     PGDGRTLVVHFDKFGNVEFTETVPDGALVCTLETFRFYLEKQGWTLVNRGAIKNMTVE
                     QLLSLK"
     gene            1320..1601
                     /locus_tag="ACHUQ7_004190"
                     /pseudo
     CDS             1320..1601
                     /locus_tag="ACHUQ7_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdaE family protein"
     gene            1637..>1750
                     /locus_tag="ACHUQ7_004191"
     CDS             1637..>1750
                     /locus_tag="ACHUQ7_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004998486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1137311.1"
                     /translation="MAGIRSTLIKEKNMNTNYEATVATTDNIVHEVYLEGKH"
BASE COUNT          500 a          399 c          411 g          440 t
ORIGIN      
        1 cagcattata tagttctact ctggcgagat acgttggcat attcatttcc ttactggttg
       61 tgtgagaact tcagtaaaga taccaccaaa gcccggaagt ggtgaaataa aaccgggcac
      121 aacacgaagg cgcatttccg gtatccataa agagtcggtc ttgtctgtga aatttaaatg
      181 gtgggagtgc gcttccggtt gtaaataacg acattgctgt gtgaagtacc agttggcggc
      241 atcggtttaa ttgctggctg atgtccgccc tttttaaagt gaattttgtg atgcggtgaa
      301 tgcggctatg cgcacgcggc acagttaaaa gtcatgttag tccttattgg tttgggtggg
      361 aaagccaact gtaattgtta actggttaac gtcacctgga ggcaccaggc accgcatcaa
      421 caaagttcac ttcggtgatg aaaggaaaga gaaaatgttg aatgtagcta ttgaaaacca
      481 gaacgggtgg aattatagtg cacctgcacc tcataaaacg ggtgccggga ttgctacccc
      541 gacgatgact actgcgcata accgcgctca ggcggttttt ttatgcgtca agcacagcca
      601 cattcagatt atggtggggc gtgcagggca gccgcaaggc tggccggttt cggtagtcac
      661 cggttgtagc aaccctgtgc gtctcaccac ccatgagatt gctacctctg gtggtgagtc
      721 atttaaactg actatcgagg ctgccatcat ggctactatc cttacccttt ctcaccctga
      781 cgcaaccatc gaaaatggtc gcgctgtcac tacgtctgtt gcggttgcgg agttcttccg
      841 gaaaatgcac aaaaacgtta ttcagaagat cgaaactctc gaatgctcgc cggagtttaa
      901 ccggcttaat tttaagcccg tcacctatac cgacgccaaa ggcgaaaaac gcccaatgta
      961 ccaaatcacc aaaaacggct tcgttttcct ggtgatgggc ttcactggca aaaaagccgc
     1021 cgcattcaag gaagcctaca ttgctgagtt cgaccgcatg gaggccgaac tgcgccagaa
     1081 taacacccca cctgcagaca agatgattcc gggtgatggt cgcactctgg ttgttcactt
     1141 cgacaaattc ggcaatgtcg aattcaccga aaccgttcct gatggcgcac tggtctgtac
     1201 cctggagact ttccggtttt atctggaaaa acagggatgg actcttgtta accggggcgc
     1261 aattaaaaat atgactgtgg aacaattact ttctttaaaa taaaaatgga gctacggata
     1321 tgaaccctat gcttgattat tgttttacct ctgcacctca gcagaccata acacacaccg
     1381 gaatcaaaaa tttgttatgg ataatcaaaa aaaacctcca gtagaaaggt gatcccgatt
     1441 taattccctg tgcttatggc ctgtgtgaca gacaagtaag ccctgataag gcttacactg
     1501 ctgaacttat ttacatgcac ggaaccatga tgaggaaaaa gaaacgcaaa tattgtagtg
     1561 aaatctgcgc cgaaaaagac cagatggcac acgaacttta attaactgac tattcgaaac
     1621 tgaatttatg ccagcaatgg cagggattcg ctcaacctta attaaggaga aaaacatgaa
     1681 caccaattat gaagccactg ttgcaactac tgataacatt gttcacgagg tttacctgga
     1741 aggaaagcat
//
LOCUS       DAZPCS010000338         1744 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZPCS010000338 DAZPCS010000000
VERSION     DAZPCS010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.344"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..353)
                     /locus_tag="ACHUQ7_004192"
     CDS             complement(<1..353)
                     /locus_tag="ACHUQ7_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFR1137312.1"
                     /translation="MNRHTQIRQSVLACLREQCGDSATFFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIFPALNDVPALSGLID
                     TLNPLGFNYQRDNEMA"
     gene            complement(350..940)
                     /locus_tag="ACHUQ7_004193"
     CDS             complement(350..940)
                     /locus_tag="ACHUQ7_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1137313.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNPPAIKLNTTRRRAGEG
                     LRVGKYFFRGAFVQQLANGRWHVLRCLPEARFATGHDHQGRLRKNRLPVEVVKIPLSG
                     PLTQAFEDARDRIIAAEMPKQLGYALKQQLRLHLSK"
     gene            complement(927..1298)
                     /locus_tag="ACHUQ7_004194"
     CDS             complement(927..1298)
                     /locus_tag="ACHUQ7_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFR1137314.1"
                     /translation="MADFDNLFDVALDLADKAIIRNMGIRAVITSGRLKGIMISGVFD
                     DPENISLVAGGVRIEDSLPSLFVKTADILRLCRNDSLMIGRESFCVDRITPDDGGCSY
                     IRLRREGLPGNVRAGRYYEGA"
     gene            complement(1310..1711)
                     /gene="gpFI"
                     /locus_tag="ACHUQ7_004195"
     CDS             complement(1310..1711)
                     /gene="gpFI"
                     /locus_tag="ACHUQ7_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFR1137315.1"
                     /translation="MAKKDDNLKRLRELAASLGREPDISGSAADIAQRVAELEEELAN
                     MDDTDIRDKSAHPENALTGHENEVISAQPETVIQNMDDLVTVVALVTLHTDALHATRD
                     EPLAFVPPGMAFRVFAGVAAGMTERGLARMQ"
BASE COUNT          393 a          527 c          407 g          417 t
ORIGIN      
        1 gccatctcat tatcacgttg atagttaaaa ccgagtgggt tcagggtgtc gatgagtccg
       61 gaaagtgccg gtacatcatt cagggccggg aaaatggtgc tctccatcca catatccagc
      121 tctgaatccg gtgcctgtgc ccggatgaag acggcaatat gcagaacagc ctgccagtca
      181 tcttcatccg tcatttttcc ggtgtactga gcatcactca gccacaccgc cacggcaggc
      241 agttcctgcg catcaataaa tgccggaagc ccgtcaaaaa acgtggcgct gtctccacac
      301 tgttcccgaa ggcatgccag cacgctctga cggatttgtg tgtgtcggtt catttactca
      361 gatgtaacct cagttgttgt ttcagggcat accccagctg tttcggcatt tccgcagcaa
      421 tgatgcggtc gcgggcatct tcaaatgcct gtgtcagcgg tccggacagc gggattttca
      481 ctacctccac aggaagacga ttttttctta gcctgccctg atggtcatgc cctgttgcaa
      541 aacgcgcttc aggaagacac ctcagaacat gccagcggcc attcgccagt tgctgaacaa
      601 atgccccccg gaaaaagtat tttcccactc tcagtccttc accagcacgc cgccgtgttg
      661 tgttcagttt gatggcgggc ggattgcccc ggttaacgcg gatcctggcc gtcatttttc
      721 ctgacggact ggctttaaac acccggacac gctgacgtac cagtttcagg gggatccctt
      781 tcacctggtt atcgcccgca acggtattcc cggcaacctg ccgggtggca accgagaccg
      841 ctttctgtgc cacacggttt atcgcccatg cgctggcctg tggcaccata cgggtatcaa
      901 ggctgttcag attgcggatg gcattctcaa gccccttcat aatatcgtcc tgcccttacg
      961 tttcccggca gcccctcacg ccgcaaccgg atataactac atccgccatc atcaggggtg
     1021 atacgatcca cacagaaaga ctcacgacca atcatcagcg aatcattgcg acacagccgt
     1081 aaaatatctg ctgttttcac aaacagggat ggtaaagagt cttcaatacg cacaccgccg
     1141 gccaccagag aaatattttc aggatcatca aaaaccccgg aaatcatgat cccttttagc
     1201 cggcctgacg taatgaccgc tctaatcccc atattgcgaa taatggcctt gtctgcgaga
     1261 tcaagtgcaa catcaaacag attatcgaaa tcagccacgc tgcctcccgt tattgcattc
     1321 tggccaggcc acgttctgtc attccggctg ccacaccggc aaagacacgg aacgccattc
     1381 ccggcggcac aaatgccaga ggttcatccc gcgtggcatg aagtgcatcg gtatgcagcg
     1441 tcaccagtgc cacgactgta accagatcat ccatattctg aatcacggtc tccggctgcg
     1501 ctgatatcac ctcattttca tgtccggtca gcgcattttc cgggtgggca gacttatccc
     1561 ggatgtcagt gtcatccata ttggcaagtt cctcctccag ctcagccaca cgctgcgcaa
     1621 tatctgctgc gcttcccgag atatccggct cacgtcccag cgatgctgcc agttctctca
     1681 gacgtttcag gttatcgtct ttctttgcca tatctgttcc tgtccgaata aaaaacggga
     1741 gccc
//
LOCUS       DAZPCS010000339         1742 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZPCS010000339 DAZPCS010000000
VERSION     DAZPCS010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.345"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            111..1475
                     /locus_tag="ACHUQ7_004196"
     CDS             111..1475
                     /locus_tag="ACHUQ7_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1137316.1"
                     /translation="MSLFTEPKEIERLPSEEIERLYPVLRYRVFISIFLGYMGYYFVR
                     NTTSVLSGVLHMSATEIGIISCAGFLSYGISKFVSGLISDRSNSKVFLSLGLFLSGLV
                     NFLIGYIPGIITSVTLFSTMYLLNGWIQGMGYPPGAKTLVFWYEHRERITWATLWNLS
                     HNVGGALAPVLIGFSFGFFGDSALDHARAAFIFPGVLCMAMSVLIYFIQVDRPVSVGL
                     PPIEEWKGNVVSHPAKGREQGPRLSIPDIIRKHIIRNNKLIYCCIYGSFVYILRYGIV
                     SWAPKFLSDSLDVGGKDMGKLASMGGGSVFEIGGVAGMLLAGYLSVRLFRNSKPLTNT
                     LFLALTIILLIAYWYVPSGNEYLWLNYTILILLGLAVYGPVMFIGLYSMELVPKEAAG
                     AASGLSGTFSYIFGSIVATLGMGLVVDYLGWGATFIVLILSAVFAIIFTLMSRERSLE
                     FEKE"
     gene            1525..1734
                     /locus_tag="ACHUQ7_004197"
                     /pseudo
     CDS             1525..1734
                     /locus_tag="ACHUQ7_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683482.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
BASE COUNT          441 a          333 c          411 g          557 t
ORIGIN      
        1 ttaaaaaaag acaactaatg agtgtgataa tgcaatactt tgagtgatta tataatgata
       61 agtaaacagg tttgcgacaa ttggttatca gtattacaga aggattaaat atgtcattat
      121 tcacagaacc taaggaaatt gaaagattac cctctgaaga gattgaacga ttatatcccg
      181 tcctgcgata cagggtcttt atcagcattt tcctcggata tatggggtat tattttgtcc
      241 gaaacaccac cagtgtgtta tccggtgtgc tgcacatgtc tgcaaccgag atcggcatta
      301 tctcctgtgc cggtttttta tcctatggta tatcaaagtt tgtttccgga cttatttctg
      361 acagaagtaa ttcaaaagta tttttgtctc ttggtttatt cctgtccgga ctggtaaatt
      421 ttctgatagg atatattccc gggatcatca catccgttac gttattttca acaatgtatt
      481 tacttaacgg ctggatccaa ggtatgggat atcctcctgg tgccaaaaca ctggtgttct
      541 ggtatgaaca tcgggaaagg atcacctggg ccacactgtg gaatttatcg cacaatgtcg
      601 ggggagctct ggcccccgtt ctgattggtt tcagttttgg ttttttcggc gacagtgctc
      661 tggatcatgc ccgggccgca tttatcttcc caggagtact gtgtatggca atgagtgtgc
      721 tgatttactt tattcaggtg gacagacctg tatcagtggg gcttcctccc attgaagaat
      781 ggaaaggtaa cgtcgtttca cacccggcta aaggccgtga acaaggtccg agactctcca
      841 ttcctgatat catcagaaaa catatcatcc gaaataataa actcatttac tgctgtatct
      901 atggttcgtt tgtttatatt ctgagatacg gtattgtttc ctgggctcct aagtttttaa
      961 gtgacagtct ggatgtcggt ggtaaggata tgggaaaact ggcatcaatg ggggggggtt
     1021 ctgtttttga aatcggtggt gtggctggta tgcttcttgc cggatacctg tctgtcagac
     1081 tgttcagaaa ctcaaaacca ttaacaaata ctttatttct ggcacttacc attattttat
     1141 taatcgcgta ctggtacgtg ccatcaggca atgaatatct ctggctaaac tatacaatcc
     1201 ttattctgtt ggggctggca gtgtatggtc cggtgatgtt tattggtctt tattcaatgg
     1261 agcttgttcc taaagaagct gccggagccg catcagggct gtcaggcaca ttttcctata
     1321 tcttcggctc aattgttgcg acactgggaa tgggacttgt tgtcgattat ctgggatggg
     1381 gggccacatt tatcgtactg atactgtctg ctgtttttgc catcattttc acactgatga
     1441 gtcgtgaacg ctcactggag ttcgaaaaag agtaattttc tcaggaaaaa cataaaatta
     1501 ctgactgaat atttattttt tgaatcgcag ggcattccgg ataaggttga tatcgtgtca
     1561 cggctgaaga cctatggata cagtatttct ggtgtggaga cggatgacgg atataaggcg
     1621 cttgtccgcg cttttcagct tcatttccgg cagaaaaatt atgacggcat aatggatgct
     1681 gaaaccgctg ccatcctgta tgcattgctg gagaagtatt tcccgggtaa ataaggagtg
     1741 ag
//
LOCUS       DAZPCS010000340         1736 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZPCS010000340 DAZPCS010000000
VERSION     DAZPCS010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.346"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..261
                     /gene="yobA"
                     /locus_tag="ACHUQ7_004198"
     CDS             <1..261
                     /gene="yobA"
                     /locus_tag="ACHUQ7_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFR1137317.1"
                     /translation="AQVTAAPQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNE
                     QDQKQLIVPLADSLKPGTYTVDWHVVSVDGHKTKGHYTFSVK"
     gene            265..1137
                     /gene="copD"
                     /locus_tag="ACHUQ7_004199"
                     /pseudo
     CDS             265..1137
                     /gene="copD"
                     /locus_tag="ACHUQ7_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            1150..1491
                     /locus_tag="ACHUQ7_004200"
     CDS             1150..1491
                     /locus_tag="ACHUQ7_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFR1137318.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
BASE COUNT          420 a          406 c          450 g          460 t
ORIGIN      
        1 gcgcaagtga cagctgcccc gcaggcaatc actttaaact tctcggaagg cgttgaaaca
       61 ggattcagtg gtgcaaaaat cacggggcca aaaaacgaaa atattaaaac attaccagcg
      121 aagcggaatg agcaggacca aaagcaactg attgttccac tggccgattc gctgaaaccc
      181 ggaacctaca ccgttgactg gcatgttgtt tcggtggacg ggcataaaac gaaaggacac
      241 tacaccttta gcgtgaaata aatcatgctg gcgtttacct ggatcgcgct gcgatttatc
      301 catttcacct cgttgatgct ggtctttggc tttgcaatgt atggtgcctg gctggccccc
      361 ttaatgattc gtcgcttgct gacgaagcgt tctctgcgtt tacaacagca cgctgccgtc
      421 tggagtttga tcagtgccac tgcaatgctc gccgttcagg gcgggttgat gggaacggga
      481 tggacagatg tattttcgcc aaacatttgg caggctgttt tgcaaacgca gtttggtggc
      541 gtctggttat ggcaaattgt tctcgccctc gtaacgttga tcgtcgccct tatgcaaccg
      601 cggaatatgc cacgtttgct gtttatgctc actaccgcgc aatttattct gctggcggga
      661 gtggggtatg cgacgctgaa tgaaggggta acagcgaaaa tccatcagac taatcacgcg
      721 attcacctga tttgtgcggc ggcgtggttt gggggattac tgccggtgct ctggtgtatg
      781 caactcatca aaggccgttg gcgacactag gctattcagg cgctgatgcg tttttcctgg
      841 tgcgggcatt ttgcggtgat cggcgtactg gcaagcggcg tgcttaatgc attgttgatt
      901 accggatttc cccccacgct tacgacttac tggggccaac tcttattgct caaagccatc
      961 ctggtgatga ttatggtggt cattgcgctg gctaatcgtt atgttcttgt accgcgcatg
     1021 aggcaggatg aagatcgtgc ggcaccctgg tttgtgtgga tgactaaact cgaatgggcg
     1081 ataggtgcgg tagtgctggt gatcatcagc ctgcttgcga ctctcgaacc tttttgatgg
     1141 attacgaaaa tgatgaaaaa aagtattctg gcgtttctgt tactcaccag ttctgcagcg
     1201 gcgctggcgg ctccacaggt gataaccgtg agtcgttttg aagtgggtaa agacaaatgg
     1261 gcgtttaatc gcgaagaggt aatgctgact tgccgaccgg gtaatgcttt gtatgtcatc
     1321 aacccaagta ccctcgtgca gtatccttta aacgatatcg cacaaaagga agttgccagt
     1381 gggaagacca atgcccaacc catttcggtg attcagattg atgatcctaa caatcccggc
     1441 gaaaaaatga gtctggcacc gtttatagaa cgagctgaaa aactctgtta attacctaaa
     1501 atagcctttt gatttccaat aaaaaaaccg cctcagttct ttcaccagaa cgggcggttt
     1561 ttaacattta agctgatgag caccgcgctt tttattgacc attttgcacg caaactggaa
     1621 aacctggcgt cgtcatctat tcttaaaggg caaggcaact aagcctgcat taatgccaac
     1681 ttttagcgca cggctctctc ccaagagcca tttccctgga ccgaatacag gaatcg
//
LOCUS       DAZPCS010000341         1733 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZPCS010000341 DAZPCS010000000
VERSION     DAZPCS010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.347"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..143
                     /locus_tag="ACHUQ7_004201"
     CDS             <1..143
                     /locus_tag="ACHUQ7_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR1137319.1"
                     /translation="AEVELATLTWVDWYNNRRLPERLGHIPPAEAEKAYYASIGNDDL
                     SA"
     gene            127..486
                     /locus_tag="ACHUQ7_004202"
     CDS             127..486
                     /locus_tag="ACHUQ7_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1137320.1"
                     /translation="MICQPEFTDKTLSRKPGAVQKVNETDAEYLVRKFDELEAKCAAQ
                     ENKVIPVSTELPPANESVLLFDANGEGWLIGWRSLWYTWGQKETGEWQWTFQVGDLEN
                     VNITHWAVMPKAPEAGA"
     gene            486..791
                     /gene="yfdT"
                     /locus_tag="ACHUQ7_004203"
     CDS             486..791
                     /gene="yfdT"
                     /locus_tag="ACHUQ7_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdT"
                     /protein_id="HFR1137321.1"
                     /translation="MTTFTDKELIKEIKERISSLDVRDDIERRAYEIALLSLEVEPDE
                     REAYELFMEKRFGDLVDRRRAKNGDNEYMAWDMTLGWIVWQQRAGIHFSTMSQQEVK"
     gene            707..1141
                     /locus_tag="ACHUQ7_004204"
     CDS             707..1141
                     /locus_tag="ACHUQ7_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1137322.1"
                     /translation="MGYDSRLDRLAATSWYPFLNNVTARGEIMEPYSLTLDEACHFLK
                     ISRPTAINWIRTGRLQATRKDPTKNKSPYLTTRQACIAALQSPLHTVQVSAGDGITEE
                     RKCHSSAEVKYGTPVSHCRTVKDLNSLLEQRTKGRRQNSMTS"
     gene            1063..>1733
                     /locus_tag="ACHUQ7_004205"
     CDS             1063..>1733
                     /locus_tag="ACHUQ7_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1137323.1"
                     /translation="MPNGKRFKQSLGTKDKRQATELHDKLKAEAWRVSKLGEIPDITF
                     EEACVRWLEEKAHKKSLDDDKSRIGFWLQHFAGIQLRDITESKIYSAMQKMTNRRHEE
                     NWKLRAESCRKKGKPVPEYTPKPASVATKATHLSFIKALLRAAEREWKMLDKAPIIKV
                     PQPKNKRIRWLEPHEAQRLIDECPEPLKSVVEFALATGLRRSNIINLEWQQINMQRRV
                     AWINP"
BASE COUNT          569 a          379 c          428 g          357 t
ORIGIN      
        1 gtgcagaagt ggaactggcc acactaacgt gggtggactg gtataacaat cgacgattgc
       61 cggaaaggct gggccacatc cctccggcag aagcagaaaa agcttattat gcttccatcg
      121 gaaacgatga tctgtcagcc tgagttcaca gataaaacac tctccaggaa acccggggcg
      181 gttcagaaag taaatgaaac agatgctgaa tacctggtgc gtaaattcga tgagcttgaa
      241 gctaaatgtg cagcacagga aaacaaagta ataccagtgt caactgaact gccaccagca
      301 aatgaaagtg ttttgttatt cgatgctaac ggagaaggct ggctaattgg ctggcgttct
      361 ctctggtaca cctggggaca aaaagaaacc ggagaatggc agtggacatt tcaggtcggg
      421 gaccttgaaa acgtcaatat cactcactgg gcagtaatgc caaaagcacc ggaggctgga
      481 gcataatgac cacttttacc gacaaagaac tgattaaaga aattaaagag cgtatcagca
      541 gccttgacgt gcgagacgat attgagcgcc gtgcttatga aatcgcactc ctatctctgg
      601 aagtagaacc agatgaacgc gaagcttatg aattattcat ggaaaagcgt tttggtgact
      661 tagtagatcg tcggagagca aaaaacggcg ataacgaata catggcatgg gatatgactc
      721 tcggttggat cgtctggcag caacgagctg gtatccattt ctcaacaatg tcacagcaag
      781 aggtgaaata atggagccat acagcctcac actcgatgag gcctgtcatt ttctcaagat
      841 atccagaccg actgccatta actggatacg cacagggcgt cttcaggcaa cacgcaaaga
      901 tcccactaag aataaatctc cttacctcac aacacgacaa gcctgcattg cggctcttca
      961 gtctccgctg catactgtcc aggtgagcgc gggtgatggc ataacagagg aaagaaaatg
     1021 tcactcttcc gcagaggtga aatatggtac gccagtttca cattgccgaa cggtaaaaga
     1081 tttaaacagt ctcttggaac aaaggacaaa aggcaggcga cagaactcca tgacaagcta
     1141 aaggctgaag catggcgggt cagcaaactt ggtgaaatac ctgatataac gttcgaggaa
     1201 gcgtgtgtca ggtggcttga agagaaagca cataaaaaat cactggacga tgacaaaagc
     1261 cggatcggat tctggcttca acatttcgca ggaatacaac taagagacat tactgaatca
     1321 aaaatttatt cagcaatgca gaaaatgacg aaccggcgtc atgaggaaaa ctggaaactc
     1381 agggcagaat catgcagaaa aaaagggaaa cctgttccag aatacacgcc aaaaccagcg
     1441 tccgttgcaa cgaaggctac gcatctttca tttataaagg ccctactaag agccgcagag
     1501 cgtgaatgga aaatgctgga taaggcacca attattaaag tgcctcaacc aaagaataaa
     1561 cggatccgct ggctggagcc ccatgaagca caaaggctga ttgatgaatg tccggagcca
     1621 ttaaagtctg ttgttgaatt tgcactggca acaggcttaa gacgctcgaa catcatcaac
     1681 cttgaatggc aacaaataaa tatgcagcgc cgggtggcat ggataaaccc gga
//
LOCUS       DAZPCS010000342         1717 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZPCS010000342 DAZPCS010000000
VERSION     DAZPCS010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.348"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(110..577)
                     /locus_tag="ACHUQ7_004206"
     CDS             complement(110..577)
                     /locus_tag="ACHUQ7_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HFR1137324.1"
                     /translation="MYGTWSLSMAAIWLLRVDLLFVALFLIFISWGDILLRIISHRYL
                     LVLTVLIFLALILQHQKPNLVAAGSVLLVGFFLFSAGIIGGGDVKLLTVLSLAIDEHE
                     LANFLVAMTFCGALVVLAGLLFFRKSIRENGVPYAVPISLAFLLTYPVFPLFC"
     gene            complement(643..882)
                     /locus_tag="ACHUQ7_004207"
     CDS             complement(643..882)
                     /locus_tag="ACHUQ7_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HFR1137325.1"
                     /translation="MNTFITKYYGKTKQCFARFAKDERGVTAIEYALIGVAMATLLAF
                     IFGDQNSGFLGAIKDAFDAIAAAIQQVTISGTSNP"
     gene            complement(1017..1622)
                     /locus_tag="ACHUQ7_004208"
     CDS             complement(1017..1622)
                     /locus_tag="ACHUQ7_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR1137326.1"
                     /translation="MSDDRFYFSLGQGVKFYPASRRITTSTGNVINLSENGYRFLLLL
                     LQGESDKQHIINEVWREQKGSVSDSSYYGQIYMLRKALDVAGLSSSLIKTIPRKGVRY
                     LGKVEKHVCENERDSDEDSSSYTNDSEAREVNTDANIPENDNSQEFSTEELLPIKHTD
                     KNIEWYRTKQWSVFISVLTVLAVCWLTTLVFLVLLFLNQDK"
BASE COUNT          549 a          378 c          305 g          485 t
ORIGIN      
        1 gcttaggcct gtgtccatat tacgtgggta ggatcacaag acctactgct atcacaatta
       61 ctgagagaaa aaaaagcatc cgatgattca tttatgctgt cctaaaaaat taacaaaaca
      121 atgggaaaac aggatatgta agcagaaaag ccaatgaaat tggtacagca taaggcacac
      181 cgttttcacg aattgatttt cgaaaaaaaa gaagacctgc cagaacaacc agagcgccgc
      241 aaaaggtcat tgcgacgaga aaattagcca gttcatgttc atcaatagct aacgaaagaa
      301 cagttaacag cttgacatca ccgccgccaa ttatccctgc ggaaaaaaga aagaagccca
      361 cgagcaaaac gctcccggct gcaaccagat tgggtttttg atgctgtaaa atgagtgcca
      421 aaaaaataag cacggttaac accagtaaat aacgatggct aataattctg agtaatatat
      481 ccccccatga aataaaaata agaaagagcg caacaaataa aagatcgact cttagtaacc
      541 atattgctgc cattgaaaga ctccatgtac catacaaggt tgctgccaga catattcctt
      601 tacgtctgac agcctctaga gagttttttt attttttata agttacggat tactcgttcc
      661 ggaaatcgtt acctgttgaa ttgctgcagc gatagcgtcg aatgcatctt taattgcgcc
      721 caggaagcct gagttctgat cgccaaagat gaaagcaagc agagttgcca tagcaacacc
      781 aatcaatgca tattcgattg cagtaacacc acgttcatcc ttcgcgaaac gcgcaaagca
      841 ctgtttagtt ttaccatagt acttagtaat aaaagtattc atgttttcct cgtatattat
      901 tatttaaatt caaaccacca gcttcatctg cgtgtcaccg gtatcccata tttttgggat
      961 gtggctataa ttatctattc ccgctattta gtcttcaaaa aaaattacat ttaatatcat
     1021 ttatcttgat taagaaatag taagactaaa aaaacaagcg tcgtcagcca gcacactgct
     1081 aacacggtaa gaacgctaat aaaaacactc cactgcttcg ttctatacca ttcaatattt
     1141 ttgtcagtat gttttattgg gagcagctct tcagtagaga attcctggct gttatcattc
     1201 tcaggaatgt ttgcatcagt gtttacttcc ctggcctcgc tatcgttggt atagctacta
     1261 ctgtcttcat cactgtcacg ctcattctca cagacatgtt tctcgacctt tcccagatat
     1321 ctgacgcctt ttcgcggaat ggttttaatt agagaactcg acaaacctgc gacgtctaat
     1381 gcctttctaa gcatgtaaat ctgcccataa taactgctgt cgcttaccga acctttctgc
     1441 tcccgccaaa cctcattgat aatatgttgc ttatcggatt ctccctgtag taaaaggagt
     1501 aaaaatcgat agccgttttc agacagattg attacattgc ctgtactggt ggtgatacgt
     1561 cgagacgctg ggtaaaactt aactccctgc cctaaagaaa aataaaaacg gtcatcgctc
     1621 ataagtaccg acccctgttt tcttcttcac atgactactc attacaactt tgtatgaagt
     1681 aagatgcaaa atcatattgt ttacattaag ttaagca
//
LOCUS       DAZPCS010000343         1716 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZPCS010000343 DAZPCS010000000
VERSION     DAZPCS010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.349"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            99..193
                     /locus_tag="ACHUQ7_004209"
     tRNA            99..193
                     /locus_tag="ACHUQ7_004209"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:133..135,aa:Sec,seq:tca)
     gene            465..1676
                     /locus_tag="ACHUQ7_004210"
     CDS             465..1676
                     /locus_tag="ACHUQ7_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1137327.1"
                     /translation="MNSSKAGGCHGINDIKVKTAKPKDKPYKLADGGGMYLLINTNGS
                     KYWRMKYRFAGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQ
                     MSLKNTFEAIAREWHQLKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMKLLEA
                     LRKMEKRGALEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTA
                     NELPHFLNDLAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVM
                     KMKRPHIVPLSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKG
                     RLTGHGFRHTMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYA
                     DYIDSLSSRES"
BASE COUNT          502 a          386 c          389 g          439 t
ORIGIN      
        1 acgctgcgtt tattaggcgg aaaaaactga tctgggggat gtagaaactc aaggaagtag
       61 ctataatgcg ccccgcctcc atgtagcaat cgaggcgcgg aagatcgtcg tctccggtga
      121 ggcggctgga cttcaaatcc agttggggcc gccagcggtc ccgggcaggt tcgactcctg
      181 tgatcttccg ccagtttacc tcttctgaac tctcccaatg tcaataaatc tcttctaatc
      241 ccttgtatat cagtatttat ttgtatcctg aggtcaacct acttcacccc gtatcaagtt
      301 tcttttgggg gtactttagg gggtgttatt tcctattgaa atacaagcac ccccataata
      361 ggaaaaatgt gatacataac aacggctttt aacaatatct ttgttataca aggggtgtat
      421 tgggggtatc aggttagtta ctggcagaaa tccccctcag gaacatgaat tcatcaaaag
      481 ccggaggatg tcatggcatt aacgatatca aagtgaaaac cgcaaaaccc aaagacaaac
      541 cctataagct tgctgatggc ggcggtatgt atctactgat taacactaat ggttctaaat
      601 actggcgcat gaagtaccgc tttgctggta aagaaaagat gctttctatt ggcgtatacc
      661 ccgatgtaac gctagccgat gctcgcgaga aacgcagtga agccaggaaa ctcctcgctg
      721 ctggaggcga tccaggggag gcaaaaaagg aagaaaaaat cgcgcagcaa atgagtctga
      781 aaaatacgtt cgaggctatt gctcgcgaat ggcaccagtt aaaagctgat cggtggtcat
      841 tacgctatcg tgatgaaatc attgatacct ttgaaaaaga catttttccc tacattggca
      901 agcgccctat tgccgaaatc aaaccaatga agttactgga agcactgcgt aaaatggaaa
      961 aacgtggagc tctggagaaa atgcgcaagg tccgccagcg ctgtggtgag gtgttccgct
     1021 atgcaattgt tactggccgg gcagactata acccagcccc cgatcttgcc agtgctttag
     1081 ctacaccgaa aaaagtacat tttccctttc ttactgctaa tgaacttccc cacttcctca
     1141 acgatctggc gggttatacc ggaagtataa tcactaagac agctactcag atcattatgt
     1201 taaccggcgt acgaacacaa gaactgcgtt ttgcacgctg ggaagatatc gattttgaga
     1261 caaaattgtg ggagatcccg gcagaagtaa tgaagatgaa gcgacctcat attgtgccgc
     1321 tctctgagca ggtcattatg ctatttaagc agcttgaacc aatctcaaaa catcatcccc
     1381 tagtctttat cggtagaaat gatccccgca aaccaattag taaggaaagc attaaccaag
     1441 taattgaatt acttggatat aagggaagac tcacagggca cggtttcaga cacaccatga
     1501 gtacaattct gcatgaacaa ggctttaatt ccgcctggat tgaaatgcag ttagctcatg
     1561 tggataaaaa ttccatcagg ggtacctata atcatgccct gtatctcgat ggccgccgtg
     1621 aaatgatgca atggtacgct gattacattg attcactctc cagccgggaa agttaagtca
     1681 gaaatacttt tccctgatgt tttcctcaat taagta
//
LOCUS       DAZPCS010000344         1675 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZPCS010000344 DAZPCS010000000
VERSION     DAZPCS010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.350"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(6..509)
                     /locus_tag="ACHUQ7_004211"
     CDS             complement(6..509)
                     /locus_tag="ACHUQ7_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFR1137328.1"
                     /translation="MIFNRLKLAVDRVIATFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGVVMMFYALCFINQSIQQLK
                     QPAQEAS"
     gene            complement(554..1517)
                     /locus_tag="ACHUQ7_004212"
                     /pseudo
     CDS             complement(554..1517)
                     /locus_tag="ACHUQ7_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
BASE COUNT          475 a          361 c          340 g          499 t
ORIGIN      
        1 atccattaac tggcctcctg cgcaggttgt ttcagttgtt gaatgctttg attaataaaa
       61 cacaaggcat aaaacatcat taccactcca cttaatggca gaatgatata tacgtatccc
      121 atcggtattt gcatagcggc agatacctgt gatgttgcca gtgttttatc aattaatttc
      181 aggccaccag tcacaatgac cgaaccggca aaacctaaaa tgagaacgtt aatgactata
      241 ctaagaagta attgtttgcg cttattcagg gcgagagcaa ataaatcaat agagagatgt
      301 cgctgcgcgc cgacggtata tgcagcacct aataatccta cccagatcat caaaaagcgt
      361 gccagctcat cggttaatgt acttggctgg ttaagtacgt agcggctaaa tacttgccag
      421 actacgcata caaccagagc aagcattacg gcgacagaaa acgtggcaat gacgcggtct
      481 accgcaagtt tcaggcggtt aaatatcatt gtaagcatcc tggaaaatca gggccagagg
      541 ctggcccgga atattattga gcagcgttgt cgaatttctc taacaaagca gcttgttttg
      601 gatctttttt gaagtcgtca aacagaggtt gcacggcttt acggaatggc gctttatcca
      661 cttttacaat ttcacccccc atagctttcg cctgagcacg tgtgtcagcg tcaatttttt
      721 cccacaattt ttgttgataa gcttcggatt tcttcgccgc tgtttcgagg atttgttgct
      781 gttctggggt taatttattc catgttttgg tggagataac caaaaagtca ggaattgagg
      841 cgtgttcatc ttcagaaaaa actttggcaa tttcaatatg tcgagtctgc atccaggaag
      901 gcacgttatt ttctgcgcca tctactacac cttgctgcat cgcagtataa acttcgccaa
      961 atgaaattgg agtgggtgaa ccacccatca gttcaatcat tttgatggtg gtcggacttg
     1021 gctgaacacg aattttcagt cctttaagat cttctggctt tgtaatcggt tttttcgcat
     1081 agaaactgcg agtacccgct acataagctg ataaagcaaa aaagcctttg tcttttgttg
     1141 agtccataat ttctttacca acttcaccaa aaacaactta ataagaacgg caaattatag
     1201 atagcataaa tattatcaaa agactccaga tcactggcgg aacctttggt catatccagt
     1261 gcgccatttt gcaacaattc catagtttct cgcgcattac ccatctggct gctgggataa
     1321 atacggatca ccatattacc ttttgacagc tgttttacct ctttcgccag ctcttcaaaa
     1381 ctctgatgta caacatgact tctttccagg ttgtgtgcta actttaacgc cactttttct
     1441 gcggcgttta cgccagtgct aaccagagcc agcaaggatg cgcagactaa tgagcgggta
     1501 aatgacctca ttttcataaa aaaactccct gttgaaggat ggaaattggt tgatatctaa
     1561 ttgtgcttat cagtgatttg tgcgacaaat tggttaacct gtctgacaac ttgtctatgt
     1621 ttatcacaac ttgaaataaa gtcagaaatt aaatattaaa tttgtgcgcc aggtt
//
LOCUS       DAZPCS010000345         1670 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZPCS010000345 DAZPCS010000000
VERSION     DAZPCS010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.351"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            311..505
                     /locus_tag="ACHUQ7_004213"
     CDS             311..505
                     /locus_tag="ACHUQ7_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1137329.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            866..994
                     /locus_tag="ACHUQ7_004214"
     CDS             866..994
                     /locus_tag="ACHUQ7_004214"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1137330.1"
                     /translation="MGADVTEFSVQGKSGTCRQFSDLFNREIISYSLSERSVMEHR"
     gene            complement(1304..1640)
                     /locus_tag="ACHUQ7_004215"
                     /pseudo
     CDS             complement(1304..1640)
                     /locus_tag="ACHUQ7_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          497 a          374 c          364 g          435 t
ORIGIN      
        1 atactggttg tctggagatt cagggggcca gtctaggtag cgtaaggcac tacagcgcat
       61 tgctttacga gtaataacaa tggatttatc accagaagac gcaaagtttt tacgagaact
      121 gcaacaccgt atgctcagag aagccattat cgaacagcaa acgggcatga gcgccataga
      181 cccattgcga tacaaacggg cctcatctcc tttagttgac gacaattcca atgcggataa
      241 aacacgtcca ccacactgat tacatttttc attagcacag tggtgtgatg cagcatacga
      301 ggaaaaccgg atgaatgaga acaaaataaa aaggctggag caactgttac aggcacgcca
      361 acgggagttt gccactaaat ggggacgtgc cgcgctgcca catgaacgcc taacctattt
      421 tccacttgaa gacctcatta aaaataatgg caaaaaaatc aggtctgata agaaacactt
      481 cttagaatgc aatgaagaca aataacaaca atctgcatat tgccaccaac cacagatgtg
      541 gcatcgttat cagcgcactt gattttcttt atattcactg acgagcttgc cggggcacct
      601 ttgtttgccg gtcattctgg tatcatgcag atatttacgg cgtacaagcc cactagcatg
      661 acattgtaat aaggcctgag cctaacgctt taatgtgtgt catacactca gtggcgtaat
      721 cgttgacaat atggtgcatg taatcgtaag cgtccagcga aatccatctg gtcataatct
      781 gaagcatccg acaaagtggt gtccaccaaa taagtgatct gcgcccaata tcctgagccg
      841 gaactttgac aaaggccaac gaaaaatggg agcggatgtt acagagttct cggtacaggg
      901 gaaaagtggt acctgtcgcc agttctccga tctgtttaac cgggaaatta tctcctacag
      961 cttgtcagaa aggtcggtga tggagcatcg ttaatacgat gctatacgat gcattcacag
     1021 tgcccggcca gaggatgccc cgtcgctgca tatggatcag ggttggcaat atcgaatcgc
     1081 aggctatagg caaagttaaa gccccatgga gtagcacaaa atatgccgca caaaggaaac
     1141 ggtctgaata acgcagtgat gaagaacttc ttcagcacac tgctaaaacg catagtgatc
     1201 gagcgctgat tctggcgaac aactgaacta atacatcaga atctgcatta tgttaaataa
     1261 atatgaaaag atggtttaaa taccccgtta cttttgactt acactatacg gtatcgcatc
     1321 gtttaatatt cgcaccggcc agatttttat ttctattagt tgtcacaata ctgaatgcgt
     1381 acgaccacag tattctggct cctgtgtggt tatgctttaa ttctgcgttc cgggcagata
     1441 agcagttgct tgcaggaatc cttcttgtgt taatgtcagt tccccttttt accagtgctg
     1501 atttccacat tccgtccaac agagcttata gcctttccct ggattatagc attgtccggc
     1561 tgaagttctt tttgaataat aatagaagca ctgctggcag atccagtccg tttttcataa
     1621 cccactgtac tgataaccat aatctaatca gtagaaattg agtcgaaaat
//
LOCUS       DAZPCS010000346         1658 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZPCS010000346 DAZPCS010000000
VERSION     DAZPCS010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.352"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..578)
                     /gene="spa29"
                     /locus_tag="ACHUQ7_004216"
                     /gene_synonym="spaR"
     CDS             complement(<1..578)
                     /gene="spa29"
                     /locus_tag="ACHUQ7_004216"
                     /gene_synonym="spaR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa29/SpaR"
                     /protein_id="HFR1137331.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIV"
     gene            complement(578..838)
                     /gene="spaQ"
                     /locus_tag="ACHUQ7_004217"
     CDS             complement(578..838)
                     /gene="spaQ"
                     /locus_tag="ACHUQ7_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HFR1137332.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLSIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            complement(853..1503)
                     /gene="spa24"
                     /locus_tag="ACHUQ7_004218"
                     /gene_synonym="spaP"
     CDS             complement(853..1503)
                     /gene="spa24"
                     /locus_tag="ACHUQ7_004218"
                     /gene_synonym="spaP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa24/SpaP"
                     /protein_id="HFR1137333.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLMEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FVVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINIPA"
     gene            complement(1508..>1658)
                     /locus_tag="ACHUQ7_004219"
     CDS             complement(1508..>1658)
                     /locus_tag="ACHUQ7_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HFR1137334.1"
                     /translation="YVENELFKFPDDIVKHVNIKVNGSLVGHGELVSIEDGYGIEISS
                     WMVKE"
BASE COUNT          665 a          321 c          252 g          420 t
ORIGIN      
        1 aatactatca taacaggact ggctaaaata acagcctgac ttgccaataa agtcagaaat
       61 gttaagatat ttgagacacg gaaagaacat tgagaaaata acgggcatat attataagac
      121 aattgtatgg attctaaaat aaagaccata ccaccactgt atagaaatac aactgcagaa
      181 aaaagattga aaaattttgc caactcagac gtatcaacac cattggcagg atcaattgaa
      241 ctactaagcg ttgccccacg ctggttgtca ataatgctac caacagcatg aaatatccaa
      301 aagggaagag acaagcaaaa agagagaagg aggccaacaa ttatttcctt gaacataagg
      361 aaataaacat gttcaaaaac agaagaacct atgtctacct taccagaagt aattaatcct
      421 gatgcaacaa gaaaaatcac aggtattcta atagatggag aaattatacc gttatttaaa
      481 aatggaagaa agaaaaataa tggagccaat ctaaaaaaaa cgccgttcag gagtattaaa
      541 aacacatgaa tactttcgaa ccagcttgaa atgtccatca aaccccactc ttaattaaaa
      601 acattatttc atgacaaaaa gacaataaaa cctcaccata ccatccagaa agaagtagca
      661 aagatattga gacacctata agctttatac caaaaggaag tgtctgctct tggagttgag
      721 tcactgtctg taacaaacca atagataatc caataaccgt agctatacct actggccata
      781 aggaaaagat aaggattaaa taaagagcct tattacccat ataaactata tcagacacat
      841 tacctccatc atctaagcag gaatattgat atattgttca atcaaggcct tagacaggat
      901 accccaccca tcaagcgcaa caaataatac aagttttatt ggtactgata ttgtgattgg
      961 actcatcatc atcataccaa gtgcaagtaa aatgcttgaa ataacaagat caacaacaac
     1021 aaatggcaaa tataaataaa aacctatttt gaacgcatct ttgatttcac ttagcgcata
     1081 tgcaggcaag agggaaaaca atgaataatc attattctct gcactcttta aatcagcatt
     1141 ttcttcttca gacctttgga aaaaacgtgc taactcaaga tctgtatgct ttttcaagta
     1201 ttgcttatat tccatcaagc cagaatcaga aaaacggaca atatcagata ttgtatcaaa
     1261 tttttgaggg ccatttagat aattttcata accagcttca ataatgggtt tcatcacgaa
     1321 caaagccata atcaatgcaa taccattgag agtcatattt gatggaacct gctgcaaacc
     1381 aagtgcattt cgtaccatta caaaaacaat agaaaacttt atataacagg taccagcagc
     1441 aactaaaaat ggtaatagcg taaaaaaaga aagtgtagcg atgagggaca tgtcactcag
     1501 catgagatta ctcctttacc atccaagaac taatctcgat accataacca tcctcaatag
     1561 aaacaagttc cccatgccca accaaagaac catttacttt aatatttaca tgtttaacta
     1621 tgtcatcggg aaacttgaat aattcgtttt ctacatac
//
LOCUS       DAZPCS010000347         1636 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZPCS010000347 DAZPCS010000000
VERSION     DAZPCS010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.353"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..1294
                     /gene="mxiD"
                     /locus_tag="ACHUQ7_004220"
     CDS             <1..1294
                     /gene="mxiD"
                     /locus_tag="ACHUQ7_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HFR1137335.1"
                     /translation="HRYPIRGNISDKTFYISGPPALVELVANTATLLDKQVSSIGTDK
                     VNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVVERLLNNGKALSNRQAQNDPMPPFN
                     ITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPETNSILVKGNDQQIQIIRDIITQLDI
                     AKRHIELSLWIIDIDKSELNNLGVNWQGTASFGDSFGASFNMSSSASISTLDGNKFIA
                     SVMALNQKKKANVVSRPVILTQENIPAIFDNNRTFYVSLVGERNSSLEHVTYGTLINV
                     IPRFSSRGQIEMSLTIEDGTGNSQSNYNYNNENTSVLPEVGRTKISTIARVPQGKSLL
                     IGGYTHETNSNEIISIPFLSSIPVIGNVFKYKTSNISNIVRVFLIQPREIKESSYYNT
                     AEYKSLISEREIQKTTQIIPSETTLLEDEKSLVSYLNY"
     gene            1313..>1636
                     /gene="mxiC"
                     /locus_tag="ACHUQ7_004221"
     CDS             1313..>1636
                     /gene="mxiC"
                     /locus_tag="ACHUQ7_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HFR1137336.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDRDFI"
BASE COUNT          566 a          243 c          322 g          505 t
ORIGIN      
        1 tcacagatat cctataagag gaaacattag tgataaaaca ttctatatat ctggtcctcc
       61 ggcattggtt gaactggtag ctaatacagc aactttgctt gataagcagg tttcttctat
      121 tgggacagat aaagttaatt ttggggttat aaaattaaaa aatacatttg ttagtgacag
      181 aacttataat atgagagggg aggatattgt catcccaggg gtggctactg ttgtagaacg
      241 tttattaaat aatggtaagg ctctgagtaa cagacaagcc caaaatgatc cgatgccacc
      301 ctttaatatt acacagaaag tatctgagga tagtaatgac ttttcattta gttcagttac
      361 caatagttct atattagagg atgtatcact tattgcatat ccagaaacca attctatatt
      421 agtcaaaggt aatgatcagc aaatacagat tattcgcgat attatcactc agttagatat
      481 agccaaacga catatagaat tatcattatg gataatcgat attgataaat ctgaattaaa
      541 taaccttggt gttaattggc aaggaacggc ttcatttggt gattcgtttg gtgcgtcatt
      601 taatatgagc tcatcagcat cgataagcac tttggacggc aataagttta ttgcttcagt
      661 tatggcatta aatcagaaaa agaaagcaaa tgtagtttca aggccggtga ttttgacaca
      721 agagaatatt ccggctatat ttgataataa caggactttt tatgtgtcat tagttggtga
      781 gcgcaattct tcgttggagc atgttacgta tggaacattg ataaatgtta tacctcgctt
      841 ttcatctcgg ggacaaattg agatgtcttt aacgatagag gatggaacag gaaatagtca
      901 gtcaaattac aattataata atgaaaatac atctgtcctt ccagaagttg gaaggaccaa
      961 aatcagtaca atagcgagag ttccacaagg caaaagtttg ttgataggtg gttatacgca
     1021 tgaaacgaac tctaacgaaa taatatctat tccttttctc tcctctattc ctgtaatcgg
     1081 taatgtgttc aagtataaaa caagtaatat cagtaatatt gtaagggttt ttctgattca
     1141 accccgtgag ataaaggagt caagttatta taatactgca gaatataaaa gcctcatatc
     1201 tgaaagagaa atacaaaaga ctacacaaat aatcccatct gaaactactt tgctggaaga
     1261 cgaaaaatca ttggtttcat acttaaatta ctaactataa agtaggtgat gtatgcttga
     1321 tgttaaaaat acaggagttt ttagctctgc attcattgat aagttgaatg caatgacaaa
     1381 ttcagatgat ggagatgaga ctgctgatgc agagcttgat tctggcttgg ctaatagcaa
     1441 gtatattgac tcatctgatg agatggcttc cgctctttcg tcatttataa acagaagaga
     1501 ccttgagaaa ctgaaaggaa caaatagtga tagtcaggaa cgtattttag atggggaaga
     1561 agatgaaatt aatcacaaga tttttgattt aaagagaacg ttaaaagata accttcctct
     1621 agatcgggat tttata
//
LOCUS       DAZPCS010000348         1622 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZPCS010000348 DAZPCS010000000
VERSION     DAZPCS010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.354"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..1302
                     /gene="ospD3"
                     /locus_tag="ACHUQ7_004222"
     CDS             <1..1302
                     /gene="ospD3"
                     /locus_tag="ACHUQ7_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HFR1137337.1"
                     /translation="FHSEKSRALEISTSNHNMALGLKIKETKNGGDFVIQLYDPNHTA
                     THLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIFVDRHTPTSMSSIIRWP
                     DNLLHPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLLAAKNDDGLSGLLLALQ
                     NGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQALQNGHAETIKTYGRL
                     LKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHADAIRAYGELILSPPLLN
                     SEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAKLNLDKKAELLEAKDSN
                     GLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEGPNGVSGLIIAFQNRNF
                     EAIKTYMEIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKS"
     gene            1419..1538
                     /locus_tag="ACHUQ7_004223"
                     /pseudo
     CDS             1419..1538
                     /locus_tag="ACHUQ7_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          542 a          309 c          328 g          443 t
ORIGIN      
        1 tttcattccg aaaaaagcag agcactagaa ataagtacat caaatcacaa tatggcatta
       61 ggcttgaaga ttaaagaaac taaaaatggg ggggattttg tcattcagct ttatgatccc
      121 aaccatacag caactcattt acgagcagag tttaacaaat ttaacttagc taaaataaaa
      181 aaactgactg tagataattt tcttgatgaa aaacatcaga aatgttatgg tcttatatcc
      241 gacggtatgt ctatatttgt ggacagacat actccaacaa gcatgtcctc cataatcaga
      301 tggcctgata atttacttca ccccaaagtt atttatcacg cgatgcgtat gggattgact
      361 gagctaatcc aaaaagtaac aagagtcgta caactatctg acctttcaga caatacgtta
      421 gaattacttt tggcagccaa aaatgacgat ggtttgtcag gattgctttt agctttacaa
      481 aatgggcatt cagatacaat cttagcatac ggagaactct tggaaacttc tggacttaac
      541 cttgataaaa cggtagaact actaactgcg gaaggaatgg gaggacgaat atcgggttta
      601 tcccaagcac ttcaaaatgg gcatgcagaa actatcaaaa catacggaag gcttctcaag
      661 aagagagcaa taaatatcga atacaataag ctgaaaaatt tgctgaccgc ttattattat
      721 gatgaagtac acagacagat acccggacta atgtttgctc ttcaaaatgg acatgcagat
      781 gctatacgcg catacggtga gctcattctt agcccccctc tcctcaactc agaggatatt
      841 gtaaatttgc tggcctcaag gagatatgac aatgttcccg gacttctgtt agcattgaat
      901 aatggacagg ctgatgcaat cttagcttat ggtgatatct tgaatgaggc aaaacttaac
      961 ttggataaaa aagcagagct gttagaagcg aaagattcta atggtttatc tggattgttt
     1021 gtagccttgc ataatggatg tgtagaaaca attattgctt atgggaaaat acttcacact
     1081 gcagacctta ctccacatca ggcatcaaaa ttactggcag cagaaggccc aaatggggta
     1141 tctggattaa ttatagcttt tcaaaatagg aattttgagg caataaaaac ttatatggaa
     1201 ataataaaaa atgaaaatat tacacctgaa gaaatagcag aacacttgga caaaaaaaat
     1261 ggaagtgatt ttctagaaat tatgaagaat ataaaaagct gaatattatc tcgtggtgat
     1321 acactgaatt tgaggtaacc ggctcattta aaccgtctgg tctgttacct ccggttccac
     1381 aaaaataatg tccatcattt tgaagtggac actatcgtat ggaataccgg acctggatca
     1441 ctgaagcttt acgccttcat ttcgaagaac atttaccacg ggttgtggcc ggacgtcgcc
     1501 tgggcgtacc aaaatcaact gtctgtggta tgttcgtgtg aaccgccccc ggtatcccgc
     1561 tcatgctggc gaacccagac acgcagtgaa ccgccccggg tttcctggag agtgttttat
     1621 ct
//
LOCUS       DAZPCS010000349         1614 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZPCS010000349 DAZPCS010000000
VERSION     DAZPCS010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.355"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..1154
                     /locus_tag="ACHUQ7_004224"
     CDS             <1..1154
                     /locus_tag="ACHUQ7_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1137338.1"
                     /translation="EDMCERMKGGISMFNHTTKTADNIEHNLQLSFLVGSRPSITNKY
                     SAKDVGPDILALPVNVYHADFKIKINNAVEKASSIVLCAVQKNLHAARKVLSGIIGNV
                     PKNNKPKTIIFDLRFLSAIKQKVFLGSEKPLFVKHTAMMREACKELPQSVEYVPLACE
                     AHSHRSVALAKTVAAPIMATGVGLALMYATSSGPYYAQSLSGSPDIEMIKNRMAELFN
                     QSDISVRNKYSPAFDKWNELVDKLNHERNTVPFACLTTILATAINETLEGDTNVAVVM
                     GCKSAKDRTISIVLGNSMLQTLLEKRLADSGEIENLFDQQGYFSCDSLTPEELMMLKD
                     LFDIRVLHVSNKFNVGLQGNINTDVLQDSFFKNVDFIHEYNSYTVGMGV"
BASE COUNT          497 a          297 c          369 g          451 t
ORIGIN      
        1 ttgaggatat gtgtgaaaga atgaaaggtg gcatctctat gtttaaccac accacaaaaa
       61 cagctgataa tatagagcat aatttgcagc taagttttct cgtgggttca cgtccgtcga
      121 tcaccaataa atactcagca aaagatgtag gtccagatat acttgcattg cccgtcaacg
      181 tatatcatgc ggattttaaa attaaaatca ataatgcagt ggagaaggct tcatcaattg
      241 tgttatgtgc tgtgcaaaaa aatctccatg cagcaaggaa ggttcttagc gggataatag
      301 gaaatgtgcc aaaaaataat aaaccaaaaa caattatttt tgatttgcgt tttcttagtg
      361 caattaagca gaaagttttt ttgggcagtg aaaaaccgtt gtttgtaaag cataccgcaa
      421 tgatgaggga ggcatgtaag gaattaccgc agtcagtgga atatgttcct ttagcctgtg
      481 aagcgcatag ccatcgcagc gtcgcccttg ccaaaaccgt ggctgcgccc atcatggcga
      541 caggtgtggg cttggcatta atgtatgcaa catcatcagg accgtattat gcgcaaagtc
      601 tttctggttc gccggatatc gagatgataa aaaatcgcat ggctgaattg tttaaccagt
      661 cggatatttc tgtgcgcaac aaatatagcc cggcttttga taaatggaat gaactggtag
      721 ataaattaaa tcacgaaaga aatacagttc cgtttgcgtg tttgaccacc attttagcga
      781 cggcgataaa tgaaactctt gagggggata cgaatgttgc cgttgtgatg gggtgtaaat
      841 ctgcgaaaga tcgcacgata tccattgtcc ttggcaactc gatgttgcaa acactccttg
      901 aaaaacgcct cgccgatagt ggagaaatag aaaatctttt tgatcaacag ggatatttta
      961 gttgcgattc attaacaccc gaagaattaa tgatgttaaa agaccttttt gatattcgag
     1021 ttcttcatgt ctctaacaaa ttcaatgttg gattgcaagg taatatcaac actgatgttt
     1081 tacaagattc gttttttaaa aatgtcgatt tcattcatga atataactcg tataccgtag
     1141 ggatgggagt ctgacgagta aaactcatta ggattatcag aattcggaca atgcattgca
     1201 aaaatgacat acaaagagag atgtgtgcag gtacttagcc gcgaggggca tggcgaagcc
     1261 atacagaaag taccagtatt cagaactcca gtgaaagcgg catagtggcc gcctgctcag
     1321 gagttttgct atgacccgta atcgtgctga acgtcgccac cctatgatgc gattaaaaaa
     1381 actcatcgtc gcgctgatga ttgacgtcaa tgctatcgga tgatgccggg ggtgttccgg
     1441 aaggaattcc ttgtttcccg aactgttacc aacaaaatta taacgatgag caagtaaaga
     1501 ccggttggtg gcagtactga ccggtctgtg atgaaagatt aatttcacct taaatcctgg
     1561 tgctgttagg atgctgacgt ttttcggtta caccattcag attatgtaaa taaa
//
LOCUS       DAZPCS010000350         1603 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZPCS010000350 DAZPCS010000000
VERSION     DAZPCS010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.356"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(335..481)
                     /locus_tag="ACHUQ7_004225"
                     /pseudo
     CDS             complement(335..481)
                     /locus_tag="ACHUQ7_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            535..717
                     /locus_tag="ACHUQ7_004226"
     CDS             535..717
                     /locus_tag="ACHUQ7_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006576 - biogenic amine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFR1137339.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
     gene            complement(818..1147)
                     /locus_tag="ACHUQ7_004227"
     CDS             complement(818..1147)
                     /locus_tag="ACHUQ7_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFR1137340.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(1319..>1603)
                     /locus_tag="ACHUQ7_004228"
     CDS             complement(1319..>1603)
                     /locus_tag="ACHUQ7_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1137341.1"
                     /translation="HFVLLNAQKLCDTQHMMQQILLSLVHALYEGNPQPVFANTEKLN
                     DAVEELRQLLDNHHDLKVVETPIYGYVWLNMETAHQLELLSNLICRALRK"
BASE COUNT          436 a          368 c          377 g          422 t
ORIGIN      
        1 atagcaatgt ctgtcgcaca acaaccacaa tgtcacaaat aaaaaatgag aataaccagt
       61 tccctgaagg cgacgtagcc cggtacaaga gtaacccacg ttagcgcttg gcgacggcgg
      121 aacaaagctg attcgcgacg taagaaagtg gcaggctcat atgacggacc tctgtaatgc
      181 ggtatccaac ccgcgaatat cagcatgatt caccgtcgga tttactgcta cgtcgccctc
      241 agggaattaa acctaaaaag aaaatcgtca aaattagaga ttccggtttg acacggaaag
      301 tcatatcagc gtcaacggag caccgtattg acgcttattt attggtgaga actacgttcc
      361 atggcaggag ttcgtcaaca cggttggagg gccattcagg cagtatgctc agaatatggc
      421 gcagatacgc ttccggatcg ataccgttca gacggcaggt gcctgctttt ccggcaacaa
      481 gcaggaatct cagcgctgaa gcaggaatta gaggtaaaaa cgccctaccg ggccatgaat
      541 cacccggtca ttggagtagt aaccaaagca gatttagcca gcatggaaca aatatctttg
      601 gtgaaaagtt ggttacggga agctggagcg cacaacgtgt tagtaaccag tgcagttaat
      661 aacaatggag ttacagaact ttttgccttg ctgcatacag aagaaggctg ttgttaattt
      721 cgattggttc tcagatgaga atgatgacgg tgatacacag ataagaatca atgagcaatg
      781 agggttgccg ggcaaccctc attgaataaa acgggaatta cttcgcttcg ccgtttttca
      841 tttcgcccat agctttcagc tttttggaga tatcgcggcg ttctttggag agctcggcat
      901 ttttgatgat gtaatcgtca acgcgatctt catagtcacc tttcatgctg gcgatgatgc
      961 cctggattgc ttcaacgctc atccctggct tgatgtaatc gctcaggttg tccagcaaca
     1021 ggacgcgctt ctggttgtca cggatctttt tctcaacgtc ctgaatttca cgttgcagtt
     1081 tgttcttacg acggaacaga cgaacaaatt ccagtacgtc ctggaatgaa ggcttggtag
     1141 tttccatttt tatacccctg ataatgtgag agtcggattc gtttaagcaa ccgctattcg
     1201 ttagggccaa cattactcat agctgccgca gatgacaatg cttttatccc ttttcactat
     1261 catacccttt atccttgctg aatcgaagca gcagcaagat gattctgaag ttcaggaatt
     1321 atttgcgcaa ggcccggcaa atcagattcg atagcaactc aagctgatgt gccgtttcca
     1381 tgttcagcca cacataacca tagattggtg tttccacgac tttcaggtca tggtggttat
     1441 cgagcaactg acgcagctct tccacagcat cgttcaattt ttccgtattg gcaaaaaccg
     1501 gctgcggatt accttcgtac agcgcatgaa caaggctcag cagtatttgc tgcatcatgt
     1561 gctgggtatc acaaagtttt tgcgcgttca ataacacgaa atg
//
LOCUS       DAZPCS010000351         1581 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZPCS010000351 DAZPCS010000000
VERSION     DAZPCS010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.357"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..1266)
                     /gene="yiaN"
                     /locus_tag="ACHUQ7_004229"
     CDS             complement(<1..1266)
                     /gene="yiaN"
                     /locus_tag="ACHUQ7_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter large
                     permease YiaN"
                     /protein_id="HFR1137342.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLLKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGIMMGATLMLTWWRQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLK"
     gene            complement(1269..>1581)
                     /locus_tag="ACHUQ7_004230"
     CDS             complement(1269..>1581)
                     /locus_tag="ACHUQ7_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HFR1137343.1"
                     /translation="VAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWG
                     ATLKTIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQG
                     A"
BASE COUNT          482 a          400 c          406 g          293 t
ORIGIN      
        1 tttcaaaggg aggatgatga gatcgggaat aaaaacaaat acgactaata atgaatataa
       61 aaccaggacg taagggaaaa cgcctctgac tgcatcatcg aatttgagtt ttgccacccc
      121 tgaaataacg ttaagtacat taccgatagg cggtgtaatt aagccgattg agcagttaat
      181 gataaacatg acaccgaaat aaatcggatc gattcctgcc tctttaacta aaggcattaa
      241 taccggggta agaattaata ccgtcggcgt taaatccatg accatgccga caatcaggat
      301 agccaccata atgacgataa acagcagacg cggtgaatcg accaacggtt gcagtaaatc
      361 agaaaccatc atcggcagtt cagcaatggt aatcagccag gcggaaactt gtgccgaggc
      421 caccagaaac ataaccactg aggtggtttt cgccgcgcca atgagtacat gccaaagtgt
      481 ggcgaaggtc atttcacggt aaataactgt ggcgacaaac agcgcataga aagcagcaac
      541 ggccccggct tcggtagggg taaacagacc ggagcgaaac ccgccaataa tgatgactgg
      601 aagaaacagc gcccagatac cggagacaaa agagtgccag atttcctgca tcgttgcttt
      661 ttgctggcgg ggtaaattaa ggcgactggc ctgccgccac caggtaagca tcagcgttgc
      721 gcccatcatt atgccagggg caatgcccgc cataaacagc ttactgatgg ataatccgct
      781 ggaaacaccg aagataataa acggaatgga agggggaata attggcgcga taatgccgcc
      841 agaggcaatc agccccgccg cccggttgac cgggtagttg gcgctgcgca tcatcggcac
      901 cagtagagcg gcaacggcgg cggtatccgc aacggcagaa ccagaaaggc tcgccataat
      961 catagctgcc agcacgccga cgtagcccag cccgcctggt ttatgcccca ccagtttcat
     1021 cggcaggtca acaatgcgtt ttaacaagcc gcccgcattc atgatttcac ccgccagcac
     1081 aaagaacgga atcgccagga gggagaagct atcggctccg ttcaccagcg tttgtgccat
     1141 gatctggaca tcaaacatgt ccagccagaa cattaacgcc gccccgcaca acaacagtgc
     1201 ccaggcaata ggcaaaccga tagcaatacc acccaacaga cagcccagaa aaatcagcac
     1261 agccatgatt aagctccttg cggtggcgac gttagagaat tgctacgcgt gatgagttga
     1321 tataaatgac gcagttcaaa gaatgcgata acgaggctgg tgggcagaca agcggcatac
     1381 atcaggccga tgggcaaacc gaggatcggt gaataatcgc tccagtcctg aattgttttt
     1441 agcgttgcgc cccaggccag tgcgccacaa ataaataaga ttaaggaatg ggtaaccaga
     1501 gcgactcgtc gttgccatgc gggggagagt ttctccacca gaaaggtgac ctgaacgtgg
     1561 gcgttatcca taaaagccac a
//
LOCUS       DAZPCS010000352         1539 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZPCS010000352 DAZPCS010000000
VERSION     DAZPCS010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.358"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..>1539)
                     /locus_tag="ACHUQ7_004231"
     CDS             complement(<1..>1539)
                     /locus_tag="ACHUQ7_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HFR1137344.1"
                     /translation="SSIFSTKNSLSTDMSVNRDNRTITSSIMRVSNSSELIQFKNKTA
                     PYFSEKRNVEVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLLSTPDAIAK
                     NVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACTLNHQMALG
                     LKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDFYWKIYGLE
                     QPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEKYKNGEISQ
                     SKLVNLLATRSSDGTPAFYIALQNGYSDIIQVYGKILNMCNLSQETILTLLAAVGANN
                     VSGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFFALQNGHAD
                     SIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKILPPHQEYI
                     DELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSATNKDGIPG
                     VFVSV"
BASE COUNT          467 a          288 c          237 g          547 t
ORIGIN      
        1 acagatacaa atacaccagg aataccatct ttattagttg cggacagcat ttcgaccaat
       61 cgtcttgtgg taaaactata gttttttaga atattacaaa aggcacgtat tgtttcggta
      121 tggccattat aaaatgccat aaaaagtcct gatgtaccgt tgggattttt tatccataat
      181 aattcatcta tatattcctg gtgagggggg agtattttta gtatatctcc atacgccata
      241 atggcctttg tgtgcccatt ttgaagtgcc ataaacaacc catgctttac tttcaataat
      301 tctttaattt gttcactact aagcattttc ttattaagca gacttccgaa catacgaata
      361 ctatctgcat gtccattctg caatgcaaaa aataacccag gtaaatcatg gctgtctttc
      421 gcagctaata gatataatct cttatcggaa gttaacgggg ttttaaagac tatttcccca
      481 taggctttta ttgtgtctac atgtccgttc ataaaagaca tgcataaacc cgacacattg
      541 tttgccccta ccgcagcaag caatgtaagt attgtttctt gactaagatt gcacatattt
      601 aatatcttac catatacttg aataatgtct gaatatccgt tttgaagtgc tatataaaat
      661 gcaggagtac catcagagct tctcgtggcc agaaggttca ccagtttaga ttgagaaatt
      721 tcaccatttt tgtatttttc catcagtata aataatactt ctgtcagatt ctttgttatc
      781 gcatgatata taactctgga attgatcaat tcatctggta aaagtttcac tgtgtttgtt
      841 agaggggaat tatgcctatc ttcaaatatt acctgcccgg gttgctccag accataaatt
      901 ttccaataaa aatcatcaaa aaaatcctta gcggataatt gactcagttc gaaatggcta
      961 tcacaagtaa atacagtacg tttatgagta acagttcgat taggatcgaa aaactgaaca
     1021 acccattttt cactttcttg tacgcgcttt attttcaacc ctaacgccat ttggtggttt
     1081 aatgtgcatg ctgataatgt tctgacaaag tctccatttt ttttcatgtt ataaaaaaaa
     1141 ttagtaataa ctgttcccca ctttttattt tccacaaaat aaatataagc tggtgatttt
     1201 gaaaaatctt ccgttttttc caaacataca ttttttgcaa tagcatcagg ggtgctaaga
     1261 agctgatagt ttaccttccc atttgtttcc ataaaattca tttcccaata ggaagctagg
     1321 tgacggcaaa ctatttgtct tccatatata tcttttgcca ctccatttat atttacctcc
     1381 acatttctct tttctgaaaa ataaggtgct gttttattct taaactgaat tagttcactg
     1441 gagtttgaga ccctcataat agaacttgtt attgttctat tatctctgtt aacagacata
     1501 tctgttgata acgaattttt tgttgaaaat atactagat
//
LOCUS       DAZPCS010000353         1532 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZPCS010000353 DAZPCS010000000
VERSION     DAZPCS010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.359"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            1..291
                     /locus_tag="ACHUQ7_004232"
                     /pseudo
     CDS             1..291
                     /locus_tag="ACHUQ7_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            293..659
                     /locus_tag="ACHUQ7_004233"
                     /pseudo
     CDS             293..659
                     /locus_tag="ACHUQ7_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            662..1060
                     /locus_tag="ACHUQ7_004234"
                     /pseudo
     CDS             662..1060
                     /locus_tag="ACHUQ7_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006278 - RNA-templated DNA biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase/maturase family protein"
BASE COUNT          379 a          380 c          461 g          312 t
ORIGIN      
        1 tcaacgtatt accactgtca gcaacagcga catcatcatg ataaacgcag tgcccgtgcg
       61 cagcgcgatg actggctgaa gaaagagata ctgcgcgtat acgatgagaa tcatcaggta
      121 tacgctgtgc gtaaagtctg gcatcagttg ttacgggaag gtatcagagt ggccagatgc
      181 actgtggcac gtctcatggc ggttatgggg cttgccggtg ttctccgggg taaaaaggtc
      241 cacactaccg tcagccggaa agccgttgcc gcaggcgacc gcgtaaaccg tcatcaaggc
      301 aatgtgccgc gtactccggg tggcccgcag cggctggtat acgtggtgtc agcggcggac
      361 aaggataaac acacgtccgc agttccgtca gcactgcgac agcgttgtcc gcaaggcttt
      421 tacccagtcc agcgttacgg tgccccacgc ctgacggatg aactgtgtgc tctggttacc
      481 actttaacgt aaaaaccgtg gcagccagtc tgcgccgtca ggaactgagc gcaaaagcct
      541 cccagaagtt cagcccgatc agttaccgtg cacacggcct gcctgtatca gaaaatctgc
      601 tgacgcagga tttttacgcc agtggcccga accagaagtg ggcgggagac atcacgtact
      661 attacagctc accgactgcg gggaaacacg gggcgccggg ttattgaagg tgacctgtcc
      721 agctacttcg acacagtaca tcaccggctg ctgatgaaag ccgtctgccg caggatcagt
      781 gatacacgtt ttatgagact gctgtggaaa acgccgtgtt gatgttggtc tcttccgggc
      841 ggtcagtgaa agtatgccac agggcggtgt gatctcgccg ttgctgtcga acatcatact
      901 gaatgagttc gaccagtatc tgaataaacg ctacctgagt ggaaaagcca gaaaggatcg
      961 ctggtactgg aatcacagta tccagcgagg ccgaagtact gctgtcaaag aaaactggca
     1021 atggaaacct gccgttgctt actgttgcta tgccgatgac tgtgtgccac gaaggcgcgt
     1081 cctggggacg tgagtccctg tgcagccatg cagcacaaga gatgagggaa ggccccttgg
     1141 tgtcggcgtg caggcgcagg caccaaacca tccactgctg ctgcatttaa gagatgtggt
     1201 ggtgagcgag atgggaaaag gtcggacgtg agccgatcag gtatggttcg aagagacgaa
     1261 cgaaagtgaa ccgctgatga tgtgtcgaaa gcatatagat gatgtcaaaa caatggtgga
     1321 gtcgttcctg tgggatatgt ttactgcctg cgcggcatcc ggcataaaag cggcgtgaag
     1381 tcgattcagg ctcttgtggg gaacgtggac ctgtcacccc gatgtgaagg gagaagcgca
     1441 agtggaagcc ccatgagcgc cagagtacca atgcggggta cagggacgga gcaccaagcg
     1501 attgcgcagt aaacatgctt gacaacttaa tt
//
LOCUS       DAZPCS010000354         1514 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZPCS010000354 DAZPCS010000000
VERSION     DAZPCS010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.360"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            232..360
                     /locus_tag="ACHUQ7_004235"
                     /pseudo
     CDS             232..360
                     /locus_tag="ACHUQ7_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_039289737.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            550..954
                     /locus_tag="ACHUQ7_004236"
     CDS             550..954
                     /locus_tag="ACHUQ7_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1137345.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
     gene            1017..1514
                     /locus_tag="ACHUQ7_004237"
                     /pseudo
     CDS             1017..1514
                     /locus_tag="ACHUQ7_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          424 a          367 c          399 g          324 t
ORIGIN      
        1 atcctctccc acaaagtgaa ttaaatgaat atataatgcg ttcgaagtat atgtattgag
       61 ctgtcatact gagaaacaaa ccttagggca gcacaattaa agccagaaac aactatgcaa
      121 gaaagttact aaataagtgc tcggaacagt aaaacactca ggggggactc agtccggatt
      181 atgtaatcag ataaattgcc aaacatcaca aatcaccagc cggactgagt gatgccgagc
      241 attgcaccaa taccccgtga tgaacgatgc ctgatgcaga aagctcccta taaaacaaag
      301 aacaaaaact acgaccggcg attcaccgtc atactgatgc tgcatcgtgg tgacactgtc
      361 tgtcactttc atgttgactg aacaactctg ctgcgaagaa ggtgtaccgc cggcgggatt
      421 tgattgtccg gcgtctagct tccacttccc tgatggaact gaaagttgtt atggccggtc
      481 agcttgccct gtccgcagga catgaggtgc tgtgacggac agcaccagta acggaaagtg
      541 acaatgaaaa tgaatactgc gctgatagtt gcactaatgt gtatgtggta tgcggtaccc
      601 gctgctgcaa aagagacttt actggctatg ccacgtaatt caacagaaca ttgctatgcg
      661 gagattaatg tgcacggacc atatggggtg tatttccgag tggtcccgca tccccccggg
      721 ggaaagagct gggtcgaatg taacagtgac tactattact ctgataaacc accgggagta
      781 cagatactcg gaacccgtgc agggtgtcgg gtatacggaa tctgcgggac cacctcgaca
      841 ctgcatgtgg ctggaagagg ggttgtgtgt attaaaaata tatgcagtcc acgtgggatg
      901 ataattcaca ggatacggaa acgtcctgta gtggctgtaa gcgatgagat gtgatcttac
      961 ccagcaatag tagacacgcg gctaagtgag taaactctca gtcagaggtg actcacatga
     1021 caaaaacagt atcaaccagc aaaaaaacac gtaaacagca ttcgcctgaa ttttgcagtg
     1081 aagccctgaa gcttgctgaa cgcatcggtg ttgctgccgc agcccgtgaa ctcagcctgt
     1141 atgaatctca gctctatgcg tggcgcagca aactgcagca acagatgacc tcgtctgagc
     1201 gtgaaagtga actgcccgcc tgaagcggca actggcggag caggcagagg aactggctat
     1261 cctccaaaag gccgcgacat actttgcgca gcggctggta tacgtggtgt cagcgtcgga
     1321 caggaataag ccctcgtcag cagttccgtc agcactgcga cagcgttgtc ctcgcggcgg
     1381 cctttacccg gtcaaaacag cgttacggtg caccacgcct gacggatgaa ctgcgtgctc
     1441 agggttacca ctttaacgta aaaaccgtgg cggcaagcct gcgccgtcag gggctgaggg
     1501 caaaggcctc ccgg
//
LOCUS       DAZPCS010000355         1512 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZPCS010000355 DAZPCS010000000
VERSION     DAZPCS010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.361"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            40..1437
                     /gene="ccp"
                     /locus_tag="ACHUQ7_004238"
     CDS             40..1437
                     /gene="ccp"
                     /locus_tag="ACHUQ7_004238"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFR1137346.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHTLNAGGV
                     DGRKTSIGVGGAVRPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
BASE COUNT          410 a          311 c          406 g          385 t
ORIGIN      
        1 ttctgggtca caataactca ccaccgtgag ggaagaataa tgaaaatggt ctcacgtatt
       61 accgcgatcg gcctggctgg cgtcgcgatt tgctatttag ggttatctgg ttatgtgtgg
      121 taccacgata ataaacgcag taaacaggcc gatgttcagg catctgctgt cagtgaaaat
      181 aataaggttt taggctttct ccgcgaaaaa ggatgcgact attgccacac gccttcggca
      241 gaattacccg cctattatta tattcctggc gcgaaacagt tgatggatta cgacattaag
      301 cttggatata aatcttttaa cctcgaggcc gtgcgtgcgg cactgctggc tgataaaccc
      361 gtttcgcaaa gcgatctgaa taagattgaa tgggtgatgc agtatgaaac tatgccacca
      421 acgcgttata ccgcgctaca ctgggcgggt aaggtgagtg atgaagagcg ggcggaaata
      481 ctggcctgga ttgcaaaaca gcgcgcggaa tattacgcca gcaatgatac tgctccggag
      541 catcgcaatg aaccggtgca gcccatcccg caaaaactgc ctaccgatgc gcaaaaagtg
      601 gcgttgggct ttgcgctgta tcacgatccc cgtttatcgg ctgatagcac catttcatgc
      661 gctcattgcc atacgttgaa tgcggggggc gtcgatggca gaaaaacatc gattggtgtt
      721 ggtggcgcag ttaggccgat taatgcgccg acggtattta actcagtatt taacgttgag
      781 cagttctggg atggtcgtgc ggcaacattg caggatcagg caggtggacc gccgttgaac
      841 ccgattgaaa tggcgtcgaa atcctgggac gaaattattg ctaagctgga aaaagatccg
      901 cagcttaaag cgcagttcct cgaagtttat ccgcaaggtt tcagtggcga aaatattact
      961 gatgccattg ctgaatttga gaaaacatta attacgccgg attccccatt tgataaatgg
     1021 ttgcgtggag atgaaaatgc gctgacggcg caacagaaaa aaggctatca attatttaaa
     1081 gataataaat gtgcaacttg tcatggtggt attattctcg gcggacgttc ctttgaaccg
     1141 ttggggctga aaaaagactt taactttggg gaaattacgg cggcggatat tggtcgtatg
     1201 aatgtgacta aagaagagcg tgataaattg cgtcagaaag tacccggttt acgtaacgtt
     1261 gctttaacgg caccgtactt ccatcgcggt gacgtgccga cgctggacgg ggcggtgaaa
     1321 ctgatgctgc gctatcaggt aggcaaagag ctgccgcagg aggatgtgga tgatatcgta
     1381 gctttcctgc acagtctgaa cggggtgtac acgccgtata tgcaggataa acaataatta
     1441 atttgatcgc ccgaacagga atgtttgggc gatttttatt acgataataa agtctgtttt
     1501 taatattatc at
//
LOCUS       DAZPCS010000356         1485 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZPCS010000356 DAZPCS010000000
VERSION     DAZPCS010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.362"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            71..352
                     /locus_tag="ACHUQ7_004239"
     CDS             71..352
                     /locus_tag="ACHUQ7_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFR1137347.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLMEKLKHAAEKIRSQFA"
     gene            318..1395
                     /locus_tag="ACHUQ7_004240"
                     /pseudo
     CDS             318..1395
                     /locus_tag="ACHUQ7_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          512 a          294 c          333 g          346 t
ORIGIN      
        1 tattaaaaat caaccacacc cgccagtatt ctgtatattt actggcggtc atatcgtgag
       61 aggtatggca atgaatcttg tgacactcaa aacgtgggga aaactcagat atccggataa
      121 cccaccatca atatcaacgc tgagacgatg ggcaaggaat ggaaacattt atcctgcacc
      181 tgaactacac gggaggagtt acagggtggt tccggaggct ttctatatca acccaaataa
      241 ggttgatacc gatataacac accatcagcc taatgggcga caagggagag acagtccgtt
      301 aatggagaag ttaaaacatg cagcggaaaa aatacgatcc caatttgcct agaaacttaa
      361 catatcgaag gagagacaaa gcatattact ggcgcaaccc tctgacgaaa gaagaattta
      421 cactaggtaa aatttcaaga agagatgcag tagcgcaggc aattgaagca aatcattata
      481 tatacaaaaa ctactctcct gctgccctaa ttgaaaagct taaaggggtc gactcattta
      541 ctatggcagg ctggattgaa cgttacaaaa cgattcttat aaggagaaaa gcgtccagaa
      601 atacttataa aactcgggta aatcaactgg agacaataaa agaaaaatta ggaggggttt
      661 tactgacaga aataaccact cgccatatcg ccgagtttct tgatttgtag attgaaggag
      721 ggaaaaacac gatggcagga tcaatgcgtt ctgtgttatc tgatatgttc cgcgaagcca
      781 ttgttgaagg acgtatatct caaaatccag taacgccaac aagagcaccg aaaatagtag
      841 ttacaagaga acggctgaaa ctaaagacat acaactgcat cagggaggca gcagatcaac
      901 ttccggcatg gttcccatta gctatggact tagcccttgt aacaggacaa cgtcgcgaag
      961 acataacgaa tatgcggttc agtgatattt atgatgatcg tctccacatc aggcaaatta
     1021 agacaggaat gatgattgct atccccctgt cactcagcct tcctgtcgct ggtttacgac
     1081 ttggtgcagt agttgaacag tgccgcatgg taagtagggg ggattatcta atcagtgccg
     1141 ggattagaaa aaacagccct gacggcagca ttcacccgga cggcctgaca aagaaatttg
     1201 tcgcagccag aaaattaaca ggtatcactg ttcagtgaaa acccaccaac ttttcacgaa
     1261 atcagaagcc tggctggacg attgtacaaa gaaatatgtg gagaagaatt tgctcagcgt
     1321 ctacttggcc acacatcgga gaagacaaca aaaatgtatc ttgatgagag agaaaaaacg
     1381 tacttactgc tctgatttta acgtaaatgg attgttaaat gtattttggt tgtgatataa
     1441 ccaaaaaaga ccggaataca gaaattcgag taaatttcgg ggaat
//
LOCUS       DAZPCS010000357         1460 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZPCS010000357 DAZPCS010000000
VERSION     DAZPCS010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.363"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            81..485
                     /gene="stpA"
                     /locus_tag="ACHUQ7_004241"
     CDS             81..485
                     /gene="stpA"
                     /locus_tag="ACHUQ7_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFR1137348.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            complement(532..1056)
                     /gene="ygaP"
                     /locus_tag="ACHUQ7_004242"
     CDS             complement(532..1056)
                     /gene="ygaP"
                     /locus_tag="ACHUQ7_004242"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFR1137349.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILNGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(1066..1365)
                     /locus_tag="ACHUQ7_004243"
     CDS             complement(1066..1365)
                     /locus_tag="ACHUQ7_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFR1137350.1"
                     /translation="MTELAQLQASTEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
BASE COUNT          398 a          339 c          344 g          379 t
ORIGIN      
        1 ttgtggaaat ataataagtg atcgcttaca ctacgcgacg aaatactttt ttgttttggc
       61 gttaaaaggt tttctttatt atgtccgtaa tgttacaaag tttaaataac attcgcaccc
      121 tccgtgcgat ggctcgcgaa ttctccattg acgttcttga agaaatgctc gaaaaattca
      181 gggttgtcac taaagaaaga cgtgaagaag aagaacagca gcagcgtgaa ctggctgagc
      241 gtcaggaaaa aattagcacc tggctggagc tgatgaaagc tgacggaatt aacccggaag
      301 agttattggg taatagctct gctgctgcac cacgtgctgg taaaaaacgc cagccgcgtc
      361 cggcgaaata taaattcact gatgttaacg gtgaaactaa aacctggacc ggtcagggcc
      421 gtacaccgaa gccaattgct caggcgctgg cagaaggtaa atctctcgac gatttcctga
      481 tctaattact gaggccggat gtcgctgcta gggcgcgtcc ggcgtcgctt ctcaagctcg
      541 ttggttccac ggcatcttat ctaacaacct tgccattcca caaaaaccgc tgattcctgc
      601 aaacagtaac ccggcaccaa caaagccact taataagaag aaaccgctat ttacggtata
      661 acccagtaca acgccgttta atattaaacc acccgcagcg atctgcacct ggcgcattaa
      721 cggcaagggt tgagatttat ttaccgctac tggcaatcca gctcttttcc agccatcaat
      781 cccatcttcg agtaaaaata tttctgcggg ggcggcaatc gctgctaatt tatcggcgtt
      841 attactggtg cgtttacctg cctggcagtg gaatataatt tgctcgcgac gtaatttagg
      901 tggaagacct gactgttcca gcacggataa tggagccaga tctgcttcag gaatatgttc
      961 acgaagatat tcatcagcgt cacgaatatc gattaacttt gcgccgcgtg cgattaattc
     1021 ttgtgcatca tgcggcgaaa tggttgtcaa agccatagtg actccttacg gacaatagac
     1081 atttttcagg gtggcgataa tggcatttac cgcctcattt ttaatggaat atagaatgcg
     1141 ttgggcatcc cgttggctgt tgataagccc ttcgtcccgc atacgagcga gatgctgtga
     1201 tgtcgcagag gcgctcagtc cggtaatgcg cgtcagctct cccgcgctgg tgccggggga
     1261 accgctaagc atgcacagaa tcagcaaccg tttagggtgg ctcattgctt tcaataaggc
     1321 cgctgcctgt tcggtactgg cctgtaattg cgcgagttca gtcatattta tttaagtatt
     1381 ttctaaatta agtaaactct aaactaaaaa tgcaacatat accagcctca gcagcgtaaa
     1441 tgagagtaaa agcgtaagct
//
LOCUS       DAZPCS010000358         1444 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZPCS010000358 DAZPCS010000000
VERSION     DAZPCS010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1444
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.364"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            72..224
                     /locus_tag="ACHUQ7_004244"
                     /pseudo
     CDS             72..224
                     /locus_tag="ACHUQ7_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          460 a          257 c          282 g          445 t
ORIGIN      
        1 tgatgggact aatcatttta atctctatac tcaaatataa acattaccat gaacaatata
       61 attgttccta agtggaatgt ctccacggag aacactttat ctatcgggaa atagtgcggg
      121 caacagtgtt taattatatc gagtgcaatt acaatcgctg gcgacggcac cgttggtgtg
      181 gcggcctcag tccggaacaa tttgaaaacc agaacctcgc ttaggcctgt gtccatatta
      241 cgtgggtagg ctcacagagc ctcacttacg actgcgtcca tattatgtgg gtaggatcaa
      301 taaagacgtt ttatatgtgt ctgcttgcag ccagaaaatt tataccctgc aggtatcata
      361 aacatgcttg ataacttaat tggggctccc tcttcctttt atggtgtaat tgtttgattg
      421 aatacttccg gggatttcag tatgaaatga agtatattta atatacttta caaaaagttt
      481 gtattagtgg aataaggaca atagcttatc caaactgcca agcctcttta ttataagtaa
      541 gatctggcac tggaatattc tcaaaaggac taacatcatc accacgacct tcaaccaacc
      601 attttattgc atttaaatcc cagaatagtt cagtagtggt caatttggga aacgcacaat
      661 attgtacggt tacgttatcg caatagagtg aactctcgcc ctatacggcg ttttcaagga
      721 ttagtcctta atcggacaac cacattaaag cataataaat aattaagctt taataatata
      781 ctatagatac agttctatta taaaaaatac tattttaggt gatgcattaa attagtagaa
      841 tatttattct tttataaaag caccaatgaa ttgtccaagt aaacatgaga taaaatgtgt
      901 agaggataac tgagtttggt cactttaact gtattagtcg cagtctgact ctcgacttta
      961 aaaggatggg aattacctgt actgatagtg agaaagcttt ccattgaaat aagtcaaata
     1021 aaagttgatg gcttcagtgc taaagtgagg tgcccgctag ccgtattcac tcgaatcgga
     1081 attcaatgta ttgcttctgc catttccata atatttcacg ggaaaaacaa aatctgaaaa
     1141 actatactta agtgttgtat tgagtcagtt gagatacaaa tacatattta tcagcacacg
     1201 ttacgtcaaa tacaggaggt tgtcatgttg gtactgaaag gcacgttggc tattgcagcc
     1261 gtgatgacaa tttattgtct tatcgttgtt cttatggaac gcttttctga ttgatttcat
     1321 attggcgaga gaacaagaag gtgagtagaa ttgtcgcgga atcctgaaac tatcagcctc
     1381 aggtatgaac accaaaaggt aggtagagaa atctatggcc cccgttactt tattcgcaca
     1441 atga
//
LOCUS       DAZPCS010000359         1442 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZPCS010000359 DAZPCS010000000
VERSION     DAZPCS010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.365"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(133..399)
                     /gene="ospE2"
                     /locus_tag="ACHUQ7_004245"
     CDS             complement(133..399)
                     /gene="ospE2"
                     /locus_tag="ACHUQ7_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE2"
                     /protein_id="HFR1137351.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPAANRTKTLKFTATEMQWLTKINEAGIDEKQSQRYSDF"
     gene            complement(581..982)
                     /locus_tag="ACHUQ7_004246"
                     /pseudo
     CDS             complement(581..982)
                     /locus_tag="ACHUQ7_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
BASE COUNT          403 a          281 c          284 g          474 t
ORIGIN      
        1 atcgaggata gatggagtta ctcctctctg aaatagaatt actgctctac tttttctgtt
       61 tccagaaatt attagtaaca aaccctgata tattcttttg aaaatggagc ttaaacgagt
      121 ctgaaattaa cttcagaaat cagaataccg ttggctttgt ttttcatcaa ttccggcttc
      181 atttatcttt gtcaaccatt gcatttctgt agcagtgaat ttcaacgttt tcgtccggtt
      241 ggcagcaggt atttgtaatt cgtatatagc tgctttttta ttgaaaacag tatatccatc
      301 tgtagtaaca gtagaagata ataatacagg atttgatact tcaaggatga tattttcttg
      361 tttttgtgga aggcaaggga atatagtttg tgtaagcata ttgcttcctt atgttatatt
      421 ttagtaaaga atttattttt tacattatag tcggctaaat gaagtttact gtaaaaaaac
      481 gatacttttt tcgtaagcga tgagatgtgg agccagaaaa atggctgcgt tacgtcattg
      541 aactgtcctt gacccacgat attcagacag tcgttgtatc ttaagttgac attacccgct
      601 gacgtcgata ttcccttggt gagagataac ccaacgcact atgcggatgg ttttcattgt
      661 aatgctcgat cgccactgca aggttatgca atgccgttct tggtttcggc atgaacgcga
      721 tgcagtcttc cttcatcgtt ttcatgaacc gttctgctat tccattactc tgagggctac
      781 tgactgccgt cgtgcagggc tccagattca actctctggc gaactgccgt gtttcatgag
      841 ccgtataagc cgaaccgtta tcggccactg gattggccgt tccggtcctt tatcgccata
      901 tcgctcctga catgacatcc tgcaccgttg ctttgtcata atctcccgtg cctgctgccc
      961 agtctatggc ttcacgatcg caatatggaa cgtaacgctg tcatcgggag aatcgtcaat
     1021 gcggcattcg gttaacgctt accaatatca acaccaaccg gatttgtgga gcctccagtc
     1081 tgaaggggct ttatgcgttc cgtataggat attatgatgc tggagttttg cgaagctcca
     1141 ctcctttccg aacatttaca cagatattgc ctaagaaaag tgaaaccata atatggttat
     1201 agtccctttc agtgcaaata cttagcttgt tgcacagaga aatagaagct gcataagctc
     1261 tttcttcaaa aaatgtaaat aaagttaaat ataggaaaaa ttacttaatc tatcttaata
     1321 acggaaagat tttgtataca atcactgtta cagaaatttc ttagttactc tgtaaacact
     1381 aaatatagtt tggtatattc tgttgaattt atgatggata tgggacataa aaacaaaata
     1441 ga
//
LOCUS       DAZPCS010000360         1434 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZPCS010000360 DAZPCS010000000
VERSION     DAZPCS010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1434
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.366"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            431..600
                     /locus_tag="ACHUQ7_004247"
                     /pseudo
     CDS             431..600
                     /locus_tag="ACHUQ7_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706798.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            746..>1434
                     /locus_tag="ACHUQ7_004248"
     CDS             746..>1434
                     /locus_tag="ACHUQ7_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR1137352.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQE
                     LCGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNINHAAMNTLLGERV
                     MDRMVMNGGRWV"
BASE COUNT          347 a          331 c          445 g          311 t
ORIGIN      
        1 tgccaacaca aaggcaacta ataaagatac agataaagat ctaaaaacag atctaaccca
       61 tcccaaaccc ttcccttccg gaagggagtt tcgggatttt gtggctggag tgcttgaggg
      121 gagattatct ggcggtactg cagcggaatt ttgtaattct gcggtggttg cgttgcaggc
      181 tgctggcctg gatgtctgtc gtgagtatcc ggtgccagag cgtggtgacg gttgcggagg
      241 gcggattgat atcgtcgtga ctgacaggaa cggtgtccgg tgtgggatcg agcttgaccg
      301 aaattctccg cgacagaaat cactgctcaa aatcggtgct gttgaaaccg ggatatgtgt
      361 cttgcggcgc agtgatatcg caaggcacac cgagcaggga attctggtta tcggtggggc
      421 tgttcgccag aaaaaatttg acccgttgtc agttgatctg cccgactggt tgtcagaaac
      481 actctggcat gagtgggtcc agttcaggca ggcattgcga aaaccgattc gaacggagct
      541 gggcgctaac ggggcgatac gggaactgga aaaattccgt cagcagggtt ttacacctga
      601 gcaggtgatt cgacacagca tcgccaatga ataccagggt ctgttcgcgc cgaaaggtgt
      661 tcggcctgag acgttgctcc gacaggttaa caccgtctcg ttgccggaca gtgcgatccc
      721 gccaggcttc agggggtaac agaccatgaa aaatattgcg acaggaggcg ttctggagcg
      781 tatccgcaga ctgaccccac cacatgtaac cgccccattc agaacggttg cggagtggcg
      841 cgagtggcaa cttgctgaag gccagaaacg ttgcgaggag atcaaccgcc tgaatcgtca
      901 gttgcgggtg gaaaaaatcc tgaaccgctc cggcatccag ccgttgcacc ggaagtgttc
      961 gtttgcgaat taccgggcgc agaacgacgg tcagcgacat gcactgagtc aggcgaaatc
     1021 catagctgac gaactgatga caggctgtac gaattttgtg ttcagcggta agcccggcac
     1081 cggaaaaaat catcttgcag ccgcgattgg caaccggctg atggcgaagg ggcgtagcgt
     1141 gattatcgtc accgtgtccg atgtcatgag cgtgttgcat gagagctacg acaacggcaa
     1201 atccggtgaa aaatttttac aggagctttg tggtgttgac ctgctggtcc tggatgaaat
     1261 tggcatgcag cgggatacga aaaacgagca ggtggtactg aaccagattg ttgatcgccg
     1321 gacggcatcg ttacgcggtg tggggatgct gacaaatatt aaccatgcag cgatgaatac
     1381 acttctcggc gagcgggtga tggatcgcat ggtcatgaac ggcgggcgct gggt
//
LOCUS       DAZPCS010000361         1428 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZPCS010000361 DAZPCS010000000
VERSION     DAZPCS010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1428
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.367"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..856)
                     /locus_tag="ACHUQ7_004249"
     CDS             complement(<1..856)
                     /locus_tag="ACHUQ7_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFR1137353.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNHVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNV"
     gene            964..1242
                     /locus_tag="ACHUQ7_004250"
     CDS             964..1242
                     /locus_tag="ACHUQ7_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1137354.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1243..>1428)
                     /gene="gadE"
                     /locus_tag="ACHUQ7_004251"
     CDS             complement(1243..>1428)
                     /gene="gadE"
                     /locus_tag="ACHUQ7_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFR1137355.1"
                     /translation="CFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKL
                     KLRRMSDIVTLGITSYF"
BASE COUNT          334 a          338 c          372 g          384 t
ORIGIN      
        1 atacattctg gcggctacct tcatccacta atgccgtgac atacatgcca ggcagcaagt
       61 caccatttgg gttggggaaa atcgcccgta acgtcacgga gcccgtggtt tcatcaaccg
      121 tcgggtcgga gaatttcagc gtgccggtct ggctgtaacg tttaccattt tccagattga
      181 gctgtactgg cgtactgccc tgaacctgtt tgatttgccc actggcgacc tcttctttca
      241 tgcgtaagaa atcttgcacc gactgcgtga gatcgacata aatcgggtcc agacgttgta
      301 cggtaaccag cgaatctgcc tgattagcgg taacgagtgc gccgacggtc accgacgatt
      361 tcccgctgac gcccgtaatc ggcgaggtga cattcgcgta ttgcagattg atcgtcgcct
      421 gttcaacagc cgctttggcg acggtgacat tggcttctgc ttcattcagc tgggtgcgcg
      481 cggtgtcgta atcctgacgg ctaacgtggt tggtcttcag caacgatgcc tggcggttaa
      541 aggtgatgcg ggcattgctg gcggtagaga gcgctttcgc cagcgagcct ttggcggagt
      601 ttagctcggc ctgtaaaggt gcaggatcaa tctgatacag cgaatcgccc tggttcactt
      661 tatcgccttc gataaagttg cgtttaatga taataccgcc cacctgggga cgtatctcgg
      721 caacttcata aggaacggtt ctaccgggca attcgctcaa cacattgacc gaaccagggg
      781 agagtgtgac gacaccgacc tcaggcgtca tggcggcggc gttttccgcc gatttgtcat
      841 cgcaggcggt gagcatcgcg ccgcagaata acaacggtat taacagcttt cttcttctgt
      901 tcattttagt ccctgaaaat tcttgagaaa taacggttct ttaaattgcg cgcgaggaga
      961 gatatgaggg atttacagac cagcggcata gtggggttgt cagccagtaa ggtgggatac
     1021 aggcacagtg atcgacatgg tgaggtcaac gacaaaaaaa atgttgccgt tctgccaaca
     1081 gttcctgcca gcattcgggc aacgaagggt tctactggtg gatacacata ccaggggaat
     1141 caagtcgttt tagcctctcc tttagtaatc accgggggta atgaaatcag catctgcatc
     1201 cctcgtcatg ccagccatca atttcagttg cttatgtcct gactaaaaat aagatgtgat
     1261 acccagggtg acgatgtcgc tcatacgtct tagtttcagc ttgtacataa tgttgaattt
     1321 ctgaatcttt aatgtttgtt gcgaaatatt gagcgtcgac gtgatattgc tttcattttt
     1381 atttttcaga atcagttgca taattttgaa ctgattatgt gaaaaaca
//
LOCUS       DAZPCS010000362         1426 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZPCS010000362 DAZPCS010000000
VERSION     DAZPCS010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.368"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(15..647)
                     /gene="mxiE"
                     /locus_tag="ACHUQ7_004252"
     CDS             complement(15..647)
                     /gene="mxiE"
                     /locus_tag="ACHUQ7_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system transcriptional
                     activator MxiE"
                     /protein_id="HFR1137356.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            complement(777..1205)
                     /gene="mxiM"
                     /locus_tag="ACHUQ7_004253"
     CDS             complement(777..1205)
                     /gene="mxiM"
                     /locus_tag="ACHUQ7_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HFR1137357.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCALKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
     gene            complement(1189..>1426)
                     /gene="mxiM"
                     /locus_tag="ACHUQ7_004254"
     CDS             complement(1189..>1426)
                     /gene="mxiM"
                     /locus_tag="ACHUQ7_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system protein MxiM"
                     /protein_id="HFR1137358.1"
                     /translation="LHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYVDLHEGFYAS
                     LSIYNNIDFYIKTTTFDSLISVFEAGREADDSTW"
BASE COUNT          479 a          268 c          182 g          497 t
ORIGIN      
        1 tcaaagattt aatattaaat tttttcattt atttttttca ctaaaaaagt aatattcgat
       61 aattctcttg cagaaaagcc tagtcgtgtt tttatctcat ttgaaaaatg ggatgttgat
      121 gcatacccat tattcatggc agctgaagtg attgtttgat tgtgcaaaaa tacatctaat
      181 aaaccattta ctaaccgcca agtgttcagt tgttctttga cttttgcccc taaagctttt
      241 ctacataaag atctaaaata agcctccgac acaccataat gctctgtcag actctttatt
      301 cttacctcct tttctttctc aatagttctc agaagataaa gaaccaaatg atatgactca
      361 gtcattcgta tcatagccag tgcattactt acattctggt cagatgaaaa gatgaatctt
      421 aacattaact tatcaagcat cgattcggta ttacaactat cccgaagatc attctcatga
      481 tagaaatgaa tacatttttc agcatggcta tgctctacta gagcaccact gaaatagtcc
      541 caaaaagcaa gaaattttgc acaactatta atgttaaagc gataatgata gttcacatta
      601 tcactgaatt ttatattttg atttcggaca aaaaaaaacc cttccatgct ggagccagct
      661 caattatgtt acgttcttta tcttttacaa gctcaacgtt aactggttca tgtccactag
      721 agtaaatgta aaacatatta cttgtattta gacctttata tttactcaca atacaattat
      781 atccctcgaa ttaattttaa tttaacttcc ccatccttct ctataaggat ggtatcattt
      841 atcttattac cttttaaaac aaccccgtct ttcagcatag aaaatagttt atctatagct
      901 atcttttgtg catccatgaa gcacccatct acttccttaa cattaaagtt gcccatgaat
      961 ttacctgcat taaaactata aaccgcctta ccactaatac atttagagta aacatttata
     1021 cttttattgg ttgtattaaa cgaccataat aaatcatttg gaatagaaaa ataatcctta
     1081 cttacaggaa caatatgcca ttctttttca gaattagatg atgactttaa agcacaccca
     1141 ctcagtgtta atagcaatat acttaaaata aatattttca acttattact accatgtcga
     1201 atcatctgcc tctctccctg cttcaaagac actaattaat gaatcgaagg ttgtagtctt
     1261 aatatagaaa tcaatattgt tatatatgga caatgatgca taaaatccct catgcaaatc
     1321 gacatattga tttgcatcta tgagtttatt aagaagtgaa cttttttgtg cagctgtaag
     1381 ataaggaaaa cgtgaattga ataattgttc aatcttattg tgcagt
//
LOCUS       DAZPCS010000363         1414 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZPCS010000363 DAZPCS010000000
VERSION     DAZPCS010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1414
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.369"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..294
                     /locus_tag="ACHUQ7_004255"
     CDS             <1..294
                     /locus_tag="ACHUQ7_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFR1137359.1"
                     /translation="SAYPIFDRIEEMAWARHYQQIVREEKETELADDLEKGLPQHLFE
                     SLCIDHLQRHGANKQAISHAFDDDVEFQERMAEHIRYMAETIAHHQVDIDLED"
     gene            300..1397
                     /locus_tag="ACHUQ7_004256"
     CDS             300..1397
                     /locus_tag="ACHUQ7_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR1137360.1"
                     /translation="MALFQRAINTQAYLKAGIMGFAGDGKTYTASELAIGLVMLMRQR
                     GIEAGNRPVMFLDTETGSDWVKPRFDAENIELFTAKTRSFVDLLEAINEAESSGSVMI
                     IDSISHFWTGLCDEYARRRNRKRGLEFSDWAWLKQEWRRFTDRFVNSQAHIIMCGRAG
                     YEYDFFEGDDGKRQLAKTGIKMKAETETGYEPSIRIQMEKQMNIETGQVWRTARILKD
                     RSTRIDGQVFSNPTFKNFLPHIESLNLGGEHPGIDTSRDNSELFANDGTPTWLKEKRA
                     KEIALDEIIELLNKHHGGTSNDAKRAKADLLEKTFTSRSWERIKGMDWPTIKAGRNAL
                     WIELEGVEYAFPDPQTQNETQQAGYDENIPV"
BASE COUNT          438 a          320 c          347 g          309 t
ORIGIN      
        1 agcgcttatc cgatttttga tcgtattgaa gaaatggcat gggctcgtca ctaccagcag
       61 atcgttcgtg aagaaaaaga aacggaactg gcggacgacc tggaaaaagg tctgcctcag
      121 cacctgtttg aatcgctctg catcgaccat ttgcaacgcc acggtgccaa taaacaggca
      181 atcagccacg catttgatga tgatgttgag tttcaggagc gcatggcaga acacatccgg
      241 tacatggctg aaaccattgc ccaccaccag gttgatattg atttggagga ctaaaatcta
      301 tggcgctttt tcaacgagca ataaacacac aggcctatct aaaagcagga attatggggt
      361 ttgctggcga cggcaaaacc tacacagcaa gcgaactggc tattggtctg gtaatgctta
      421 tgcggcaacg gggaattgaa gctggtaacc gcccggtaat gtttctggac acagaaacag
      481 gctctgactg ggttaaaccc cgctttgatg cggaaaatat cgaattattt acggcaaaaa
      541 cccgctcgtt tgttgatctg cttgaagcca ttaatgaagc ggaaagcagc ggttccgtaa
      601 tgatcatcga ctccatcagt cacttctgga ccggattatg cgacgagtat gccagacgcc
      661 gaaaccgtaa acgaggactg gaattttcag actgggcgtg gctcaaacag gagtggcgac
      721 gctttaccga tcgatttgtt aacagtcagg cgcacattat tatgtgcggg cgtgcaggat
      781 atgagtatga ctttttcgaa ggtgatgatg gcaaacgcca gttggcaaaa accggtataa
      841 aaatgaaagc agaaacagaa accggttatg aaccctccat ccggatccag atggagaagc
      901 aaatgaacat agaaaccggt caggtgtggc gcaccgccag aatactaaaa gatcgctcca
      961 cccgtattga tggacaggta ttttcaaacc caacatttaa aaactttctt cctcatatcg
     1021 agtctcttaa cctgggaggt gaacaccccg gcatcgatac ctcacgggat aacagtgaat
     1081 tatttgccaa cgacggtaca ccgacatggc taaaagaaaa gcgtgcaaaa gaaatcgcac
     1141 ttgatgaaat tatcgagttg ctcaacaagc accatggggg caccagtaac gacgccaaac
     1201 gcgcaaaagc tgatttgctg gaaaaaacat tcacctcccg ctcatgggaa agaatcaaag
     1261 gtatggactg gccgacaatc aaagctggtc gtaatgcact atggattgaa ctggaaggcg
     1321 ttgaatatgc attcccggat ccacaaacac aaaacgaaac gcaacaagca ggatacgatg
     1381 agaatattcc ggtgtaaaac caagtgcatt ttgg
//
LOCUS       DAZPCS010000364         1394 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZPCS010000364 DAZPCS010000000
VERSION     DAZPCS010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.370"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(1..702)
                     /gene="lpfB-O113"
                     /locus_tag="ACHUQ7_004257"
                     /pseudo
     CDS             complement(1..702)
                     /gene="lpfB-O113"
                     /locus_tag="ACHUQ7_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_316045823.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB-O113"
     gene            complement(750..1322)
                     /gene="lpfA-O113"
                     /locus_tag="ACHUQ7_004258"
     CDS             complement(750..1322)
                     /gene="lpfA-O113"
                     /locus_tag="ACHUQ7_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HFR1137361.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVNFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          384 a          318 c          289 g          403 t
ORIGIN      
        1 tgcgccgtaa tcattaataa atcgccaggt catttggcct cccgcagaat tagcaggtaa
       61 aggtaacgtg agcgtttcaa aaggacggat catattgagg ggcacatcat ggccatcaac
      121 gatgacatcc atcacactga tatgaattgg gttgggattc tctgctatca gatttcgccc
      181 ttcccggcgc catttcactt tcggcgcagc atcttcggga ataaggcgga tatttgctgg
      241 tcgccagaac agcttgatac gcgtttttac tgctatttcc agcgagttcg tcgctttgtt
      301 gctcgctgat ggcggaatat ttttaatatt cagccagaag actgactcgc gatcatcagg
      361 caatgacaca cctttggtgt gaataatgcg taatattttt tcttgcgaag gttctaatcg
      421 tgatactggc ggaataacgg taaacggcgc tttgctttta tcctctttat taaatggatc
      481 tacccaagcc tggatgagat aaggtatttt ttcttctttg ctaaatacag aaatagattt
      541 atcagcatta ttacttaagt aaaccacccg cgtactacca atgacaacgc ctgcataagt
      601 taatgctggt tggcctaaaa aaacgctgag tataatcgcg gtaaataact tacattaaac
      661 tgttgtttta aagagcctga ttaaacaact ttttctggac ataacgactc cctggccaaa
      721 gccaactgaa taaaaaggcc cttgataaat tatttcttat attcgacagt aaattgtgaa
      781 tcagcgttcg ccgtaccagc cgttacggtc gcattggttg ccacatagcg tgcaataaac
      841 ttcatttcag ccttgccttc agcatcgata gcggtataag cagagtcgtt ataaagttta
      901 acctgcgaat tatcggactg gttaaaaata cgaataccaa cgccggttgc cgcaatcgct
      961 tgtcctgtac cggtaaaatc accagggttg cctgctgtaa gcggcgtacc cactttcagg
     1021 tcgccgtcgc cgccaggcgc ttctgtacca tcaaaacgaa cggctgcttg agtgtacgtt
     1081 gccgggcaat tttcaagttt aatactgaac ttctgtggcg acgccagact acctacacct
     1141 gaaaatgcat ttttactgac tttacccagt gggacaattt gatcggctgt atcggaggta
     1201 acttcacaag tagtgtcgac aatttcaccg acgaagttaa caacaccatc ttctgccagt
     1261 gcatgcccgg ccagcattag agatgccaga gtgaatgcac cgcctataat attacgcttc
     1321 ataaaaactc cattgatata atatatcctt taccatgaat catcacttat attaataagt
     1381 gtgtttttga ttca
//
LOCUS       DAZPCS010000365         1366 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZPCS010000365 DAZPCS010000000
VERSION     DAZPCS010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.371"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..246)
                     /locus_tag="ACHUQ7_004259"
     CDS             complement(<1..246)
                     /locus_tag="ACHUQ7_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005137803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1137362.1"
                     /translation="MHHSGVVNISSDNLQNILSKPREHTLNCLGWRFFVQSSVCVSWK
                     SDFAWTGIMLLFRGEELAFSSAFSPVTIRKIMKCPSGE"
     gene            complement(427..501)
                     /locus_tag="ACHUQ7_004260"
     tRNA            complement(427..501)
                     /locus_tag="ACHUQ7_004260"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(466..468),aa:Gly,seq:tcc)
     gene            complement(505..579)
                     /locus_tag="ACHUQ7_004261"
     tRNA            complement(505..579)
                     /locus_tag="ACHUQ7_004261"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(544..546),aa:Thr,seq:tgt)
     gene            complement(582..658)
                     /locus_tag="ACHUQ7_004262"
     tRNA            complement(582..658)
                     /locus_tag="ACHUQ7_004262"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(622..624),aa:Arg,seq:tct)
     gene            complement(660..736)
                     /locus_tag="ACHUQ7_004263"
     tRNA            complement(660..736)
                     /locus_tag="ACHUQ7_004263"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(700..702),aa:Ile,seq:cat)
     gene            complement(926..>1366)
                     /locus_tag="ACHUQ7_004264"
     CDS             complement(926..>1366)
                     /locus_tag="ACHUQ7_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFR1137363.1"
                     /translation="GGKTGNMFNLMLTSKKLTKTAINEALRRMKKAGLNKSELEAFLR
                     DMINGKQKSWLAHCTDAEALCIDRVISEVLAEHPGLISVLRQRYEGRGMTKCKMAELL
                     NDAHPKWSLRTCERRIEHWLKVAEFILYKPMVMAFGIEKKVIAF"
BASE COUNT          369 a          361 c          284 g          352 t
ORIGIN      
        1 ctccccggag gggcatttca ttatttttct aatcgttact ggtgagaagg cggaagaaaa
       61 agccagttct tcgcccctaa ataacagcat gattccagtc catgcaaaat ctgatttcca
      121 gctcacacat accgagctct gtacaaaaaa ccgccagcca aggcagttaa gagtgtgttc
      181 ccggggtttg cttagaatat tttgtaagtt gtccgaagag atatttacaa caccagaatg
      241 atgcattatc agtcccctgc cagaggttca ctacgtaacc tgaccgacaa tgcaccattc
      301 tggtgctgaa aaaagagcac tggaactgca acaaaacaac atcacagaac aggagatggt
      361 aaggagtcag acattcccac acaatattgt gtcagtgcaa ataacaacct tcgtctcaaa
      421 tctcgctgga gcgggcagcg ggaatcgaac ccgcatcatc agcttggaag gctgaggtaa
      481 tagccattat acgatgcccg catatggtgc gactaccgga atcgaactgg tgacctactg
      541 attacaagtc agttgctctg cctgctgagc taagtcggcg ctggcccacc accgaggact
      601 cgaacctcgc accgtcaact tagaaggttg atgctctatc cggatgagct agtggtggtt
      661 ggtggccctt gctggacttg aaccagcgac ctggcgatta tgagtcgctc gctctgacca
      721 actgagctaa agggccggag gcagaataat aaccatatgt catcacatct gcaaactcat
      781 ctgatcacca gcacgtttaa cgtcctgtgc cgtttttcag gtataaaaaa aaccgcataa
      841 agcgggctct ttcaaatgtc catgtctgct attcgcctcg cggtacagct ttgcgaagcg
      901 tacaagaatt gaagcagttt ttacgtcaaa aagcaataac ttttttctct ataccaaaag
      961 ccataaccat tggtttgtac aaaataaatt ctgccacctt tagccaatgc tcaatgcgtc
     1021 tttcacaggt tcttaaactc catttcgggt gtgcatcatt cagcagttca gccattttgc
     1081 atttggtcat cccccgcccc tcataccgtt gccgaaggac gctaatcaat cctggatgct
     1141 ctgccagcac ctcacttatg acccgatcaa tacataacgc ctctgcatca gtacaatgcg
     1201 ccagccagct cttttgctta ccgttaatca tatcccgcaa aaaagcctca agttcagact
     1261 tgttcagacc tgcttttttc atcctccgga gcgcctcgtt aattgccgtt tttgtcagct
     1321 ttttagaggt caacatcagg ttaaacatat tccccgtctt accgcc
//
LOCUS       DAZPCS010000366         1356 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZPCS010000366 DAZPCS010000000
VERSION     DAZPCS010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.374"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(41..100)
                     /locus_tag="ACHUQ7_004265"
     CDS             complement(41..100)
                     /locus_tag="ACHUQ7_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR1137364.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            complement(339..1265)
                     /locus_tag="ACHUQ7_004266"
                     /pseudo
     CDS             complement(339..1265)
                     /locus_tag="ACHUQ7_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin domain-containing protein"
     gene            complement(1281..>1356)
                     /locus_tag="ACHUQ7_004267"
     CDS             complement(1281..>1356)
                     /locus_tag="ACHUQ7_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR1137365.1"
                     /translation="LSFSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT          274 a          361 c          326 g          395 t
ORIGIN      
        1 acaagggtga gggaggattt ctcccccctc tgattggctg ttaataagct gcgaaactta
       61 cgagtaacaa cacaatcagt atgatgacga gcttcatcat aaccctttcc ttctgtaagg
      121 ccccttcctc gggaggggct ttcccgtttc agccctttgc tgactccccg attgttgatg
      181 agcgccggac ctcgcgcaac gctttcccag aggcagtttt gccttccccg agcggtcacg
      241 caaaacgttg caacgacctg atcatttgca gtttttagcg gaacgcagat ataaaaaatc
      301 cccggctttt tgtgccaggg acgttcatag ctgaaaattt attttacgtt ttcggcgacg
      361 ggagttgttg agattaaggg tgaagtcaat gccgccagtg aaccgcctga aacgccaatt
      421 tcattgagca aggagtcaat cagcggagcc tgtgtgcggt aagagagagc ggctgataat
      481 gcttgttccg ctaggtttcc gccccccaca ttacccgtgt ttgcatcacc cgcagcgctg
      541 ccactattca gaccatcgac ctgaataatc ttgataccgt caatagattt catcggctca
      601 acggattttt ctattaccgc aggtagcgcc tgcaacaagg ccagtttgaa tttaaggctg
      661 gtttgttcat cagaaagtac gttgatagca tcgttcagcg cacgttgtgc ttctgcttcc
      721 gccagaccct ttttatgtgt agcttcggct aactcaacga tagccgccgc ctgcatctct
      781 gcggcttctt tttctgcttt cgcccgcacg gtcagttcaa ccgctttggt ttctgcatcc
      841 tgcgcggcag cgattagggc aacttgttta gcacgatcgg cttctgcggt ctggcgagtg
      901 gtttctacgt tttgttgcgc acttactgct tctgcgagtg caaggttagc acgcgcttct
      961 gcctgggact gttgttccga tttggcggca atagcgatcg atttcgtctg gttggcgatt
     1021 tcggtgacct gctgctgttc gatctcttta atacgaactt cacgttcagc ctcaaccttt
     1081 cttgagcgga cggcctgctc gcgctcgatc tccgtttcct gaatttgtcg ttcagccaga
     1141 attcgcgtct gctctgcttc acgacgacgt tcagcttcaa aagccgcaat acgtgcattc
     1201 tgttcggcag tacgggtttt aacctgctgc tcctgctcaa gcgtcataaa cgcttcttgt
     1261 tgttcggtaa tgactccaac ttactgatag tgttttatgt tcagataatg cccgatgacc
     1321 ttgtcatgca actccaccga ttttgagaac gacagt
//
LOCUS       DAZPCS010000367         1355 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZPCS010000367 DAZPCS010000000
VERSION     DAZPCS010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.375"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            183..1322
                     /gene="pdeF"
                     /locus_tag="ACHUQ7_004268"
                     /pseudo
     CDS             183..1322
                     /gene="pdeF"
                     /locus_tag="ACHUQ7_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
BASE COUNT          315 a          293 c          300 g          447 t
ORIGIN      
        1 acatgataaa cgtaataatt cattaatcag ggttattttg tttgatatat atcaattgga
       61 actttcatat gacacttaga atcaatgctc tctatagtga tgaataatca tcattcgaag
      121 tcaggtggga tgcctgtctg aatacacctc cttcaggatg tgggggattc tgctgagcat
      181 ctatgaaact gaatgcaact tatataaaaa tacgtgataa atggtggggg cttccgctgt
      241 tcctgccttc tttaatcttg cccattttcg cccacattaa tactttcgcg catatttctt
      301 ccggtgaggt ttttctcttt tatctgcctt tggcactgat gatcagcatg atgatgtttt
      361 tcagctgggc ggcattgcca gggatcacct tagggatttt tgtccgcaaa tatgcagagc
      421 tgggttttta cgaaacgctc tcattaacgg ctaattttat tatcattatc attctctgtt
      481 ggggcggtta cagggtcttt actccccggc gtaacaacgt ttcacatggt gatacccgtt
      541 taatttccca gcgtatattc tggcagatag tgtttcctgc aacgctgttt ctgatacttt
      601 tccagtttgc tgcatttgta ggattactgg cgagcagaga aaatctggtc ggtgtcatgc
      661 cttttaacct cgggacttta atcaattatc aggccttact ggtgggtaat ctgatcggtg
      721 tcccgctgtg ctacttcatc attcgggtag tgcgaaatcc attttattta cgtagctatt
      781 attcgcaatt aaaacagcag gttgatgcca aagtcaccaa aaaagagttc gcgctctggc
      841 tactggcatt aggtgcttta ttgttgctgt tatgcatgcc gttaaatgaa aaaagcacaa
      901 tttttagcac caattatacc ttgtcattat tgctgcccct gatgatgtgg ggagcaatgc
      961 gctatggtta taagctgatt tcgctgctct gggcggtcgt gttgatgatc agcatccaca
     1021 gctatcaaaa ttacattccc atttatcctg gctataccac gcagctgacc ataacctcct
     1081 ccagttatct ggtattctct tttattgtca attatatggc tgtactggca actcgtcagc
     1141 gagcggtagt cagacgcatt cagcggcttg cgtatgtgga cccggtggtt catctgccaa
     1201 atgttcgcgc cctgaatcgc gcgttgcgtg atgccccctg gtctgcgctt tgttatttac
     1261 gcatccctgg catggaaatg ctggttaaga actatggcat catgctgcgg attcaatatg
     1321 aggtgtactg gcaatagcgg acactaccat ttgtt
//
LOCUS       DAZPCS010000368         1338 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZPCS010000368 DAZPCS010000000
VERSION     DAZPCS010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.376"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            80..820
                     /gene="phoN2"
                     /locus_tag="ACHUQ7_004269"
     CDS             80..820
                     /gene="phoN2"
                     /locus_tag="ACHUQ7_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family phosphatase PhoN2"
                     /protein_id="HFR1137366.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAENSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDEKMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
     gene            893..1015
                     /locus_tag="ACHUQ7_004270"
     CDS             893..1015
                     /locus_tag="ACHUQ7_004270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1137367.1"
                     /translation="MGTPRFTPEFKGLLERLGHIPPAEAEKAYYAAIGNDDLAT"
     gene            complement(1173..1338)
                     /locus_tag="ACHUQ7_004271"
                     /pseudo
     CDS             complement(1173..1338)
                     /locus_tag="ACHUQ7_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
BASE COUNT          400 a          284 c          265 g          389 t
ORIGIN      
        1 tgacttgtgt tttttgtaat acagctcgtt ttttacatct gaccaaaatc atcaattaat
       61 tatgctaagg aaataaatta tgaaaaccaa aaactttctt cttttttgta ttgctacaaa
      121 tatgattttt atcccctcag caaatgctct gaaggcagaa ggttttctca ctcaacaaac
      181 ttcaccagac agtttgtcaa tacttccgcc gcctccggca gagaattcag tagtatttca
      241 ggctgacaaa gctcattatg aattcggccg ctcgctccgg gatgctaatc gtgtacgtct
      301 cgctagcgaa gatgcatact acgagaattt tggtcttgca ttttcagatg cttatggcat
      361 ggatatttca agggaaaata ccccaatctt atatcagttg ttaacacaag tactacagga
      421 tagccatgat tacgccgtgc gtaacgccaa agaatattat aaaagagttc gtccattcgt
      481 tatttataaa gacgcaacct gtacacctga taaagatgag aaaatggcta tcactggctc
      541 ttatccctct ggtcatgcat cctttggttg ggcagtagca ctgatacttg cggagattaa
      601 tcctcaacgt aaagcggaaa tacttcgacg tggatatgag tttggagaaa gtcgggtcat
      661 ctgcggtgcg cattggcaaa gcgatgtaga ggctgggcgt ttaatgggag catcggttgt
      721 tgcagtactt cataatacac ctgaatttac caaaagcctt agcgaagcca aaaaagagtt
      781 tgaagaatta aatactccta ccaatgaact gaccccataa agctggacag cctgtatcag
      841 gctatggagg gcccatagac aaatctaccc tatatgagca taggaggagt ctatgggcac
      901 accacgtttt acccctgaat ttaagggatt actggaaagg ctgggacata tccctccggc
      961 agaagcagaa aaagcttatt atgctgccat cggaaacgat gatctggcaa cctgagttca
     1021 cagataaaac attctctagg aaactcgggg cggttccgtt caccacatgc aatgtggtgt
     1081 tgcaggggaa cggtctgcac catcccctat gtcgatcaat ataacagaaa tgacaacttc
     1141 agattcaggg cacaacctaa atatatttta ggtcacctct caaatcgttt gcctgatacc
     1201 gctccatttt ttaataaaaa aatcaatcat ttttgaatct ttgcttttca ttgcattgtc
     1261 aagcatagtg tccccactat ttgctttttg aaattttttg tttacatcaa ccaatccatt
     1321 attgataaaa tattctag
//
LOCUS       DAZPCS010000369         1336 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZPCS010000369 DAZPCS010000000
VERSION     DAZPCS010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.377"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(207..362)
                     /gene="hokD"
                     /locus_tag="ACHUQ7_004272"
     CDS             complement(207..362)
                     /gene="hokD"
                     /locus_tag="ACHUQ7_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR1137368.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESRK"
     gene            complement(590..1255)
                     /locus_tag="ACHUQ7_004273"
     CDS             complement(590..1255)
                     /locus_tag="ACHUQ7_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFR1137369.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASEIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
BASE COUNT          331 a          342 c          314 g          349 t
ORIGIN      
        1 ccatccctct tctccctgcg cgctacttaa gtgagtcgat tctatctgcg aaggtgcgcg
       61 aatcaaattc accggaaata aacaacaaaa aacccgccga aacgctgaga gatcacagcg
      121 atgggcgggt taagtgcggg tgcgttgagg atgcctgaca catcagaggt gggcggggat
      181 gggatcagct ccccacccgg tcactcttac ttcctggatt cgtagtctac gaagacagcg
      241 acctccgtct ggccggttcg gattcgtacc tcacagaggt ctttcctcgt taccagtgcc
      301 gtcactatga cggttaaaca gatgacgatc agggcgatta acatcgcctt ttgctgcttc
      361 atagcctgct tctccttgcc tttcggcatg taagaggcta acctacatgt gcaaagcatg
      421 aaattggcct cagattaatg ttaagcgtct tgccggacgc gtaatgttaa ctggggcttt
      481 tctctatctg ccttttggtg ttcatgcctg aagcagatag cctcaagcac ccgcagtcat
      541 tctacttacc tcgccaatat gaaatcaatc agaaaggtgc cccataaaat cactccttct
      601 cttctttacc gtagtggagt tgaccaattt tgataagagg gcgtccctga gatttgcggt
      661 gtagattggt atcgcgcaga gaatacacac agccacaata ttcctgctga tagaattttt
      721 cgcgcttgct gatttcaatc atacgggacg agccgccctg cttgcgccag ttataatccc
      781 agtacaccat gcccggataa tgcgcgacgg ctcgccgccc acagtcgtta acctgctgca
      841 tatttttcca gcgtgaaatg cccagtgaac tgctgatcac actgaaacca ttttcagcgg
      901 cgtacagcgc tgtccgctca aaacgcatgt caaaacacat ggtgcaacgg atccctcgtt
      961 cgggctccca ttccattcct ttggctcgtt caaaccagtt gtctgtgtcg taatcagcat
     1021 caataaacgg cacgccgtgt tgttcagcaa agcgaatatt ctcatcctta cgaattaaat
     1081 actctttctg aggatgaatg ttcgggttgt agaaaaagat ggtgtagtcg atttccgagg
     1141 cctgaagcgc ctccatcacc tcaccggaac atggagcaca gcaagagtgc agtagtagtt
     1201 tgtttgcccc gtttgggagc tccaatttgg gccgtttgaa atcagcaact gtcataaata
     1261 tgtttattga ggtcataaaa atatcaaaga gtgtagcatt agaacggggc tatcggaaac
     1321 agatgtgtga ctgctc
//
LOCUS       DAZPCS010000370         1321 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZPCS010000370 DAZPCS010000000
VERSION     DAZPCS010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1321
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.378"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(355..>1321)
                     /locus_tag="ACHUQ7_004274"
     CDS             complement(355..>1321)
                     /locus_tag="ACHUQ7_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFR1137370.1"
                     /translation="EMFVFLAVELSLLFIVISAGVSLIRQKVPDHKIQQMMGARKGRG
                     YLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLNPIIVGLMWMTFG
                     WKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIKV
                     SCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFIY
                     GFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMALI
                     IGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT          396 a          278 c          284 g          363 t
ORIGIN      
        1 aaaaagttat tatgttctcc gatagtgtgt gaagtaatat ttttgtgatg ttttagatgt
       61 gtaataatga accaggttaa agtgtattta aaaatgtgtt gtattagcga ctgaaatctg
      121 ccttttaaaa aggaggaggg tactgagaca aagagtcatt gcttctccga taaaatgtca
      181 gagaagcatg attacgtggt tattgtctgt tgatgacttg ttgaccgagc cgtcatcatt
      241 ctcaatgcat tagttaaatc atttgtgatt tttaaggatt atcctgtcta tgattttcta
      301 cttagaagga tatggcacta actatgcatc ttatagttta ctgctggcat ctatttaaaa
      361 tagcaattga gtcaaatagc ccatcaagat agccatacct aatataaccg tcaggaatgc
      421 agctatcatc ggcattctga acattgattt aagtaatatc acttccgtca ggcttgcgcc
      481 ggcactgccg atgattaatg ccattaatgc tcccagaccc attccttttg tcatcagaac
      541 agatgccaga gggataaccg cctcagcccg gatatacagc ggaataccga caacggcgct
      601 taatgggatg gcgaagggat tatctgctcc tgcatgagcg gcaatccacg ctgagggaat
      661 aaagccataa ataaaagatc ctattaatac tcctaacaaa agatatggca gtacatcttt
      721 aaactgttgc aacgcatctt tggtggctag ttttatggga ttaatactta ctacagcttt
      781 ggtactgcaa caggtaacta cggggttctc aatggcttta gccgctggac tacaacaact
      841 cacttttatg ggcgtatatg tcgttcccgg cgaagttttt gctggagcac aacaatttgc
      901 tgatgagctt ttactggcaa tgatatgacg ttcaaacccc agagaatcca ggataatact
      961 ggcaagaacg gagacgccgg ctgcgataat cgcgtacaac aaggtgactt tccaaccaaa
     1021 ggtcatccac attagtccga cgataatggg attaagtaat ggtgaaacaa ataaaaaagt
     1081 gagagtcgga ccaaacccgg ctttcgctga caacaatcca cgtaacatgg gtattgtcga
     1141 gcaactacag aacggggtaa cggctcccaa cagagcagcc aggagatagc ctctcccttt
     1201 tctggccccc atcatctgct ggattttatg gtctggcacc ttttgtctta tcaggctgac
     1261 accggcacta atcactataa acagcaacga aagctcgacg gcgagaaaca caaacatctc
     1321 t
//
LOCUS       DAZPCS010000371         1288 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZPCS010000371 DAZPCS010000000
VERSION     DAZPCS010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.379"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..1236)
                     /locus_tag="ACHUQ7_004275"
     CDS             complement(<1..1236)
                     /locus_tag="ACHUQ7_004275"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFR1137371.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKQGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTL
                     YDLSERLRLRGWQVPAFTLG"
BASE COUNT          301 a          344 c          343 g          300 t
ORIGIN      
        1 gccgagagtg aaggccggaa cctgccagcc gcgcagacgc agacgttcag agaggtcgta
       61 cagggtgtat cccggatctt caccatcttt cagtttgaag caaaccgccg ggatgccttc
      121 gtccgggcga cccgtacaga tgaactcata cggcccctgt ttggcgattt catccgccag
      181 ataagcggca acctggtaag aggcgttctg tactttggta tagccttcac gaccgaggcg
      241 caggaattca tagtactgtg caattacctg acccgccggg cgggagaagt tgatggcaaa
      301 agtaccaatt tgaccaccca ggtagtcaac gttgaacacc agttcctgcg gcagcgcttc
      361 ttcgtcacgc cagataaccc agccgcagcc cagcggagcc agaccgaatt tatggcctga
      421 agcactgatc gatttcacac gcggcaggcg gaagtcccag acgatatccg gggcgacgaa
      481 cggtgccagg aagccaccgc tggcagcgtc gatgtgcatg tcgatgtcga taccggtgtc
      541 ggcctggaat ttatccagcg catcgtgcag cggttgtggg aactcatagt taccggtgta
      601 ggtcacgccg aaagtcggca ccacgccgat ggtgttttcg tcacaggctt caatcatgcg
      661 tttcgggtcc ataaacaact gaccggggcg catagggatc tcacgcagct ccacatccca
      721 gtagcgggcg aatttatgcc agcagatttg taccggaccg cacactaggt ttggtttatc
      781 cgttggtttg cctgcagctt ccatacgctt gcgccaacgc catttcatcg ccatcccgcc
      841 gagcatacag gcctcggaag aaccaatggt gttggtgcca acggcctgac catttttcgg
      901 cgcaggcgca tgccacagat cggcaaccat atttacgcaa cgcaggtcga tggctgcgga
      961 ttgcggatat tcttctttgt cgatccagtt tttattgatc gacaaatcca tcaatttatg
     1021 gacgttttcg tcgtcccagg tctggcagaa agtggctagg ttctgacgag cgttgccatc
     1081 aagatataat tcatcattga taatctggaa tgcgacatca tcgcgcattt cgtgcagcgg
     1141 aaatcgtttt gactccgcga tagtagaaat ggcctttgcg ccaaaacgtg aatcgagtag
     1201 ttctgagcgg aaatccgtta acagcttctg gtccatttcg aactccttaa atttatttga
     1261 aggcaataaa aaagtaggat ttatccgc
//
LOCUS       DAZPCS010000372         1284 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZPCS010000372 DAZPCS010000000
VERSION     DAZPCS010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.380"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            522..806
                     /locus_tag="ACHUQ7_004276"
     CDS             522..806
                     /locus_tag="ACHUQ7_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="HFR1137372.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQSWADSLSTDHLLPVPR"
     gene            806..1081
                     /locus_tag="ACHUQ7_004277"
     CDS             806..1081
                     /locus_tag="ACHUQ7_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFR1137373.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIYVLRLWHTRENR"
BASE COUNT          339 a          321 c          324 g          300 t
ORIGIN      
        1 acagaataag tttacgcgta aatcgccctg aatccagaag agcattaagt atcacataaa
       61 caggttccat accagtcctg accgcatcag aacgccagta aataaaatct cgtagtccac
      121 cggcatcaga gacaagagta atcgtcgtta ccggtatttc gactggtttg tactggtgtg
      181 agcggacaaa actactgtac gtctctcata cacgctcgta agcggccctc acggcccgct
      241 aacgcggaga tacgccccga ctgcgggtaa acccttgtcg ggaccactcc gaccgcgcac
      301 agaggcattt tcatcgctga caggccgcat ggcctcgcag agccggaggg ggactggtga
      361 tattcggacg accgtaccgg cttgcgccgg gcttcggcgg ttttacacct gtgctacatg
      421 taaccaccgt atgccgcctt atatgcgctg actcgcatta tttgccttgt tccgtctgaa
      481 agtaatcact atgattaaat atgattaaca actaatcggg tatgcaaatg aaaaacaata
      541 ccgcacaagc aacaaaagta attaccgcgc atgtgccatt acctatggct gataaagtcg
      601 accagatggc cgccagactg gaacgctccc ggggctgggt tatcaaacag gcgctttctg
      661 catggcttgc ccaagaggag gagcgtaatc gcctgacgct ggaagccctg gacgatgtga
      721 catccggaca ggttatcgac catcaggctg tacagtcctg ggcggacagc ctcagtactg
      781 accatctgtt accggtgcca cgctgatgga actgaagtgg accagtaagg cactttctga
      841 tttggcgcgg ttatatgatt ttctggtgct ggccagtaaa cctgctgccg ccagaacggt
      901 gcagtccctg acacaggcgc cggtcattct gttaactcat ccacgtatgg gagaacagtt
      961 gtttcagttt gaacccaggg aggtcagacg gatttttact ggcgagtacg aaatccgtta
     1021 cgaacttact ggccagacta tttatgtatt gcgcctgtgg cacacacgag agaacaggta
     1081 gattgggtgt ggaggcaact ttaacatagc ctgcaggtat taattctgga gcattgttat
     1141 caggccagat attttgcatt ccattaaata atcttgtagg agataaaata aattacgatg
     1201 aaataacgaa aattaccgct cgtgactggc ggcaatatcg cgctccgggc tggcaaataa
     1261 cccatcagaa acgttattgc caga
//
LOCUS       DAZPCS010000373         1279 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZPCS010000373 DAZPCS010000000
VERSION     DAZPCS010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.381"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..835)
                     /gene="ipaH4.5"
                     /locus_tag="ACHUQ7_004278"
     CDS             complement(<1..835)
                     /gene="ipaH4.5"
                     /locus_tag="ACHUQ7_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HFR1137374.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQSLKYLKVGENQLRRL
                     SRLPQELLALDVSNNLLTSLPENIITLPICTNVNISGNPLSTHVLQSLQRLTSSPDYH
                     GP"
BASE COUNT          359 a          230 c          286 g          404 t
ORIGIN      
        1 gcgggccgtg gtagtccggc gaagaggtta atctttgcag ggattgcaga acgtgagtcg
       61 acaatggatt ccctgaaatg ttaacattcg tacaaatggg caatgtgatt atattttcgg
      121 gtaatgaagt tagtaggttg ttggaaacat ccagtgccaa tagttcttgc ggtaatctgg
      181 ataatctgcg tagttgattt tcacctacct tcaaatattt taagctctga ggaaattctg
      241 gtagttcctg tagtctgttg ccggaaacaa agagttcttg tagtctatga ggcaattcag
      301 gcaagacagt aaggcgatta ccttcaatac gtaatattgt tagcgttgat ggaagctctg
      361 gtaacgtctc cagactatta aaacgtgccg agagtgattg taaatatggt ggtaatgatg
      421 gcagacatga caaatttcga ttgaatgaaa tattaagctt aatcagttga gatggaagtg
      481 tcgggagtat attcaagttg ttaccattta catgaagttc tgtcaaaagc ggagaatttt
      541 caggtaggga gattaactca ttgttggaaa tattaagttc acgtatatgc aaagggagtg
      601 gtggtaaaga acgtagtttc agtaagctta aattgagaac aggctcccgg gtatccagac
      661 atattttcaa taatcgaacc gcctggatgc gattttctgt tgttgttcct tccctagccc
      721 agtcatccca gatgcggtgg taatctctgg tacactgttc ttctaccgat aaacgagata
      781 tacatgataa gccacaaagg gaacgaaaaa aagaatgatt gttgatcggt ttcatttatt
      841 aacccctcac tattcatatc ttggtctatt aatgcaaata aaacgagaaa gtctttaaaa
      901 aaacaatcct agagctttat cggcgttttc attccggctg gaggaatatg ttcctgtctt
      961 tccagtaacc gtcgattgtt atatcagtct acccatgtta atgtgaccag ttctactttc
     1021 atccagtttg gattgatctt tatcccctat attttcaaac aatttttatc atttaactca
     1081 ttactatctc gatattccca tagcaaacga tctggttggt tatcccctca aaaaagtcag
     1141 ttgcacgccg taaatccctg ccggtattga gcgaggattt acgggttgtt gttaaggtcc
     1201 gtaatcgggt cacagttttt ccggagtcaa tccggtctgc ggtttatgct tatgcgacgt
     1261 gattatgaat ggtgcagtc
//
LOCUS       DAZPCS010000374         1262 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZPCS010000374 DAZPCS010000000
VERSION     DAZPCS010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.382"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            134..853
                     /locus_tag="ACHUQ7_004279"
     CDS             134..853
                     /locus_tag="ACHUQ7_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HFR1137375.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQSFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
BASE COUNT          376 a          238 c          252 g          396 t
ORIGIN      
        1 gcgttctaag ccttcccacc atgttcggag aggaccctta cgcttacata cattacatat
       61 aatgtatcgt tttataaaag atgataaaca atcaatataa gataatatat ctattttata
      121 gaggacgttt tctatgccca taaaaaagcc ctgtctaaaa ctaaatttag attctttgaa
      181 tgttgtgagg tctgaaatac cgcagatgct ttctgcgaat gaaagattga aaaataactt
      241 caatatattg tacaaccaaa tacggcaata cccagcgtac tatttcaaag tagcatcgaa
      301 cgtgccaact tactctgata tatgtcaatc tttttctgtc atgtaccaag gattccagat
      361 tgtgaaccat agtggagatg tgtttattca tgcatgtcgt gaaaatcctc agagtaaggg
      421 ggattttgtt ggggacaagt ttcatattag tatagctagg gagcaagttc ctctggcatt
      481 ccaaattttg tctggtttat tattttcaga ggacagtcct atagataaat ggaagataac
      541 tgatatgaat cgagtttctc agcaatctcg tgtggggata ggagctcagt ttacgctata
      601 tgtaaaatca gatcaggaat gctcgcaata tagtgcttta ttacttcata aaatacgaca
      661 atttataatg tgtctcgagt ctaatctatt aagaagcaaa atcgctcccg gggagtatcc
      721 ggcgtcagat gttagacctg aagactggaa atatgtcagc tatcgtaatg aactacgaag
      781 cgatcgagat ggaagtgaaa ggcaagagca aatgttacga gaggaaccat tttatcgttt
      841 gatgatagag tagagaagat gccagctaca ccaccttgta gcttgaaaac tggttagaat
      901 aactacgatt agatttggca ctgcgtatgc agcctgattc ctgcccgtga cagagcattc
      961 ggctcataaa ctgctgagct actcatctga aatccgaaca gttgtccacc atttcagcgt
     1021 gcatctcctg tttgagtcgt ttgattgctt ttcgggatat taacctgtcg gattttgcat
     1081 tagttaatgc tcccatcagg ccttcatctt ttccagtgcc tattttcctg ttctcctttt
     1141 gcacaatata atcagatcat ctgtatcgtt gacgagctcg ctatcttata ggagcattct
     1201 gaataagcta cctccttgta aggaggcttt tactattagt aactgcaatc taatagtatt
     1261 at
//
LOCUS       DAZPCS010000375         1257 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZPCS010000375 DAZPCS010000000
VERSION     DAZPCS010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1257
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.383"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..143)
                     /locus_tag="ACHUQ7_004280"
     CDS             complement(<1..143)
                     /locus_tag="ACHUQ7_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HFR1137376.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFT"
     gene            complement(545..1255)
                     /gene="ydeO"
                     /locus_tag="ACHUQ7_004281"
     CDS             complement(545..1255)
                     /gene="ydeO"
                     /locus_tag="ACHUQ7_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFR1137377.1"
                     /translation="MLDKDYAFSDGEILMVDNAVRTHFEPYERHFKEIGFNENTIKKY
                     LQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERNHHNFSELLLFSCLSIFA
                     ACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCLYISESLLKKKLKQEQTT
                     FSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYAFRKHFGNSPKRVSKEYR
                     CQRHTGMNTGNTMSALAI"
BASE COUNT          391 a          236 c          238 g          392 t
ORIGIN      
        1 gtaaagtctg agaaagcaac gtttgttttg gttgctttca gctttacatc atctgccgca
       61 tgagcagcaa atgcagccat accagatgcc agtatcaaag atatcgaggt acttacgaca
      121 cttttcttta atgcagactt cataaatgtt cctcttctta ttgtacttaa ttccgaagta
      181 attttacgaa gagaaataag tggatgtaag tgaagttagt cacataaaga gatagcagat
      241 ttagctaaaa aaagggaaat aacagtccat aaagcgttga cattactttc tgttctatta
      301 agtaatttct cgccgataaa caactaattt attgatattt aataaattat tgcattttac
      361 tgacaaaatg ccgaattgag atcataaata atcatgcaac aggttatgca agtgcataaa
      421 tatgtgatgg atgtcactta tttatttcaa taaatatatc gcctaaaaac aacgcggggc
      481 agggaatggc tgccccatat aattcttacg tagcgtgcgt ggttgactac tcgttagcaa
      541 ataatcaaat agctaaagca ctcatcgtgt tgcccgtatt catacccgtg tgcctttgac
      601 aacggtactc cttagaaact ctcttcggac tgttgccgaa atgtttgcgg aacgcataaa
      661 taaaataaga tgtactggca taaccacatt gttcggcaat tttattgact gaaccttcta
      721 cgcgtatcaa attttttgcg tgctgcattc tggcatctaa tagaatctgt gagaatgtcg
      781 tttgctcttg ctttagtttt ttcttcaaca ggctttcact gatgtacagg cagtcacaaa
      841 tatctttcag cttccatggg tgcgccagct tcatgttgac aatatttctc actttcccgg
      901 aaacggatag cacaccgtta gttaatagtg taatgaaacc tttgcatgcg gcaaaaatag
      961 acaggcaaga aaaaagcaaa agttctgaaa aattatgatg attgcgttct tccgagttga
     1021 gataagcaat catttcatta agcaatccag tcggtacatt tgaagcacgt aaaaacttcg
     1081 caggaacagg caccgtcact gtctggatgt tagtgcattg tagatatttt ttaatggtat
     1141 tttcattaaa tccgatttct ttaaaatgcc gctcataagg ttcaaaatgt gtacgaacag
     1201 cgttatctac catcaggatc tcgccgtcgg agaaggcgta atctttatct aaaatgt
//
LOCUS       DAZPCS010000376         1244 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZPCS010000376 DAZPCS010000000
VERSION     DAZPCS010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.384"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            199..>1244
                     /locus_tag="ACHUQ7_004282"
     CDS             199..>1244
                     /locus_tag="ACHUQ7_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR1137378.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRA"
BASE COUNT          405 a          283 c          197 g          359 t
ORIGIN      
        1 tggtataaat cctctgcatt gtgaatatgt aaaacatcaa aaaaagatga accaaatata
       61 cgaaaaaagc aaatatactt accagggtag aaagcatgat ataacactca ttcccttatg
      121 gcaaatattc tgaaacaccc tcctcattag gagagataca ttaaatacaa tttattcaca
      181 atctaattcc atatccatat gcgagaaatt aatatgctca gaaacatttc atcctgttta
      241 tttccacata tcagcacaat tacatccccc aaccattatt tgtccgaatg ggatgattgg
      301 gagaaacagg ggttaccgga agaacagcgt actgaggcgg taagaagact tcgtgcatgt
      361 cttacctcta aggggcataa actggacctg cgagccttgg cgctttcctc gctacctgta
      421 ctccctgctt gcattaaaaa gcttgatgtg agctgtaata aattaaccat ccttactgat
      481 ttacctgaaa atattaaaga acttattgca agagataatt tcttaacaca tatatctgca
      541 ttaccacatt atctaataac tttggatgtg tccgaaaatc aattagagaa tctgccgtta
      601 ttaccagaca ccatcaaatc actaagcgca gagtataata ggttatccac actgccttca
      661 ttacccttga atttaaaaaa acttgaggtt aggaacaacg aactgcaaac tcttccatct
      721 ctgccttcta atcttaagat acttaaggtt gcgcacaacc atcttactga actgccccct
      781 ttacctagga gactgcaact tctttttgca tatagcaata gattaagcaa cttaccaaac
      841 atccaagaaa atattatcat gagaagattt ttttattttg aaaacaacca aataactaca
      901 atcccgacaa atctttttcg tttagatcct catataacta ttgagattgc aaataacccc
      961 ttatcagatc aaactctgct attcttaata cagcaaactt cggttccaaa ttttaacggg
     1021 cctcagtttc gtatttccct gtcagaccaa aacagactgt ttttacgcca gatgttgccg
     1081 caaaatttac attcgcgcca tatcagagtc atcactgaag gggggcagaa ctttcagatc
     1141 ccccctcttc ccgaaactgt ggcagcctgg tttcctgaag cagatcgtcg ggaggtttct
     1201 acacaatgga cttctttttc caccgaggag aattcccggg catt
//
LOCUS       DAZPCS010000377         1198 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZPCS010000377 DAZPCS010000000
VERSION     DAZPCS010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.385"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            37..786
                     /gene="phoC"
                     /locus_tag="ACHUQ7_004283"
     CDS             37..786
                     /gene="phoC"
                     /locus_tag="ACHUQ7_004283"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase PhoC"
                     /protein_id="HFR1137379.1"
                     /translation="MKRQLFTLSIVGVFSLNTFASFPPGNDVTTKPDLYYLTNDNAID
                     SLALLPPPPQIGSIAFLNDQAMYEKGRLLRNTERGKLAAEDANLSSGGVANVFSAAFG
                     SPITAKDSPELHKLLTNMIEDAGDLATRSAKEYYMRIRPFAFYGVSTCNTKEQDTLSR
                     NGSYPSGHTSIGWATALVLSEINPARQDTILKRGYELGDSRVICGYHWQSDVDAARIV
                     GSAIVATLHSNPVFQAQLQKAKDEFANNQKK"
     gene            complement(841..945)
                     /locus_tag="ACHUQ7_004284"
                     /pseudo
     CDS             complement(841..945)
                     /locus_tag="ACHUQ7_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707145.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(956..>1198)
                     /locus_tag="ACHUQ7_004285"
     CDS             complement(956..>1198)
                     /locus_tag="ACHUQ7_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR1137380.1"
                     /translation="ELKNNGIKAVIPRKSNEKMASDGRAQLDRDAYCNRNVVERCFGR
                     LKEYRRIATRYDKTARNYLAIVKLGCIRLFYQRLRN"
BASE COUNT          322 a          267 c          259 g          350 t
ORIGIN      
        1 aattataaat atgtatcctc tataaggaga tgttatatga aaagacagct ttttactctt
       61 agtattgtcg gggtattttc tttaaatacc tttgcatcat tccctccggg aaatgatgtg
      121 acaacaaaac ctgaccttta ctacctgaca aacgataatg ctattgacag tctggcatta
      181 ttaccgcctc caccacaaat cggaagtatt gccttcctga atgaccaggc catgtatgaa
      241 aaggggcgtt tattgcgaaa tactgaaaga gggaagctgg cagctgagga tgccaatctc
      301 agtagtggtg gcgtggcaaa tgtattttct gctgcttttg gttctccgat aacggctaaa
      361 gattcaccag aattgcataa attactgaca aatatgattg aggatgccgg agacctggca
      421 acgcgttcag caaaagaata ttatatgcgt attcgacctt ttgcttttta tggtgtttct
      481 acgtgtaata caaaagaaca ggatacatta tccagaaatg gctcttatcc atcaggtcat
      541 acatcgattg gttgggcaac agcacttgta ttgtccgaaa taaatccggc acgccaggat
      601 accattctga aacggggtta tgaactgggg gacagcaggg ttatttgcgg ttatcactgg
      661 caaagtgatg ttgacgcagc acgtattgtt ggctctgcta ttgtagcaac actgcactca
      721 aatcctgtgt tccaggcaca attacaaaaa gcgaaagatg aattcgctaa caatcagaaa
      781 aaataatgct tttagccagt acgccttata accccgacct tcagggctaa tgccagtcag
      841 ttaagcaact gactggctct ttttcagggc tgtggggtat ttccagtgcc tctcctttac
      901 cactctcgga aaggcccttt cccttcttgt cggtaatttc actaggctgt gtcccttaat
      961 tacgtaagcg ttgataaaag agtcggatgc agcccagttt cactatcgcc aggtaatttc
     1021 tcgccgtttt gtcgtaacgc gtggcgatgc ggcgatattc tttcagccgc ccaaagcacc
     1081 gttcaacgac attacgattg cagtacgcat cacgatcaag ctgtgcacgt ccatccgatg
     1141 ccattttctc atttgacttt cgggggatta ctgcctttat accgttattt ttcagctc
//
LOCUS       DAZPCS010000378         1194 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZPCS010000378 DAZPCS010000000
VERSION     DAZPCS010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.386"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..320)
                     /gene="mobF"
                     /locus_tag="ACHUQ7_004286"
     CDS             complement(<1..320)
                     /gene="mobF"
                     /locus_tag="ACHUQ7_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobF family relaxase"
                     /protein_id="HFR1137381.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSISMMAMLGGDKRL
                     IEAH"
     gene            402..629
                     /gene="vapB"
                     /locus_tag="ACHUQ7_004287"
     CDS             402..629
                     /gene="vapB"
                     /locus_tag="ACHUQ7_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HFR1137382.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSTDFMDNREQPGMQERESF"
     gene            629..1027
                     /locus_tag="ACHUQ7_004288"
     CDS             629..1027
                     /locus_tag="ACHUQ7_004288"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFR1137383.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGKMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
BASE COUNT          301 a          331 c          293 g          269 t
ORIGIN      
        1 ttatgtgctt caataagacg tttgtccccc ccgagcatgg ccatcatgga gatacttttg
       61 ggagcggaga aggtcaggtc gtagccggga cgatgcctgt tactgccatc ctgcatgcgg
      121 cttaaatccg ccccgtccgg cagcctgccc tccagaaggc gggtaaagac atccttatcg
      181 acactgccct gcagccccag ttgttcagcc ccctggccgg cccagcgttc tcccatgctg
      241 cccagcacat agtaattatc cttgtcggta taataattac cggcacttcc ggccgatctg
      301 acctgcgcga tactcatcat aaacgtatat ccctttgaca tatcccggta tcaatcccac
      361 aatagatata cacaagacat atccacataa ggaggcaaat aatggaaacc accgtatttc
      421 tcagcaaccg cagccaggcg gtcagactgc caaaagcggt tgcattgccg gaaaacgtaa
      481 aacgcgttga agtgattgct gtcggacgaa ccagaatcat tacgccagcc ggagagacat
      541 gggacgaatg gttcgacgga cacagcgtca gcaccgattt tatggataac agggaacagc
      601 ccggcatgca agaaagggag tcattctgat gctgaagttt atgctcgata ccaacatctg
      661 catttttacg ataaagaaca aacccgccag cgtcagggag cgttttaacc tgaaccaggg
      721 gaaaatgtgt atcagttcgg tcaccctgat ggagctgata tatggtgcag aaaaaagcca
      781 gatgcctgaa cgtaatctcg ctgtgatcga gggatttgtt tcccgcattg atgttctgga
      841 ttacgacgct gctgcagcca cacacaccgg ccagataaga gcagaacttg cccgtcaggg
      901 acgccctgtc gggccatttg atcaaatgat cgcaggtcat gcccgcagtc gggggctgat
      961 tattgtgact aataacaccc gggaatttga acgtgtgggc ggcctgagaa ctgaagactg
     1021 gagctgacct gttcatcaga aatcatctcc cggctcaaca tcctccccgc gttcccggtg
     1081 cacattgatg ttcacctctt cacgacgctg catctgctgc caggtgtccg gatgattttc
     1141 ctgctgccat gcctcataag cggccatatc caccacttca ccggattcac tgat
//
LOCUS       DAZPCS010000379         1192 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZPCS010000379 DAZPCS010000000
VERSION     DAZPCS010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.387"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(377..1000)
                     /locus_tag="ACHUQ7_004289"
     CDS             complement(377..1000)
                     /locus_tag="ACHUQ7_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFR1137384.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
BASE COUNT          316 a          254 c          246 g          376 t
ORIGIN      
        1 tattttatcg ccacttccaa ttcctttctg catggtaagt agtgaggacg aaaagaagaa
       61 tatgataaat attaattcgt ggtaatcaga gagagtcgcg acatttgtga tgttattctt
      121 acaactgtaa gaagcgccgg tgatttacaa gtaagagtat gattacgctt tttaaagcgt
      181 accataaagc ataacataat aaatgatttg tgatgacacg ggattattgc ggtttacttt
      241 gaggaaaata aaatgcgtgt ttatagaacc gcgagcgcca aataatttta tgaccacaac
      301 ctgtcgtatt gtggtcataa aaaattgtcg gctggaggga gttaaatcct ttacctcaat
      361 atactgcctg tcagcattag ttcacaaaca gtgcttcgct atcgacgatg attccatctt
      421 ccgcaccttt aagcgccagg tttgcctcgt agtattgctt tttatcaagc agtttctgcg
      481 catcagcaag tgcattacga gtctgtttaa gcggcatcag atattggttt tccattacgc
      541 caacaccggc caggcgaagc tcttccattg cgcccttttt atcccctttc gccatttttt
      601 cattggcaat ttttatcgcg gcttcttttt cgggtgttgc tacataactc tcagaaattc
      661 cgacagatgc attaatgaca atatattggt catcattgag attggtcttt ttacctggct
      721 tagcgaactt tgcccattcg gtactgtcat ctgacagcaa agcggaagct tcattggtca
      781 gttcttttgc cttttccgga tcgccatgaa acagagccag gcgcgctacc tgcacgtcac
      841 gcatcgcata taaaccttgt tcagagatac gctctgctgc tgccgttttc tgggtttgct
      901 gagtttgctg ttgtgccgct accggtgcat tatcagcagc ccatacagtt gaagatgcca
      961 gaattgctgt gaccatcgta gccataatta aacgtttcat acttacctca ttctttaaca
     1021 gttttagaaa acgccgccat catattgggt gagcatgaaa tgagaaatca gagagcaacg
     1081 gtcattttga aagggttatc ttacaattgt agttattatc gcaaagcggc atttagtctt
     1141 acagttgtag gaatatgcct cctgtcgcag agaactattc ttatatacca ca
//
LOCUS       DAZPCS010000380         1173 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZPCS010000380 DAZPCS010000000
VERSION     DAZPCS010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.388"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..167
                     /locus_tag="ACHUQ7_004290"
     CDS             <1..167
                     /locus_tag="ACHUQ7_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HFR1137385.1"
                     /translation="AIASTPHPSWRRICKTLIKNDFWCRTLSFSPNKPRHYERYLQRM
                     KERRKEWGTL"
     gene            152..790
                     /locus_tag="ACHUQ7_004291"
     CDS             152..790
                     /locus_tag="ACHUQ7_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HFR1137386.1"
                     /translation="MGDSVTPEILGNMIRQYFSQVRTEKESIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMALGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQLLGREADAGKRLRGWLPVACINPERKGQAARIAATIRHNRARGKHQITLMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSSAWTVR"
     gene            1018..>1173
                     /locus_tag="ACHUQ7_004292"
     CDS             1018..>1173
                     /locus_tag="ACHUQ7_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1137387.1"
                     /translation="MKKQIFINNKPPVVPYSGTHAKIFKYIEIPLPFFYFIYTSGEPF
                     HISVQNTV"
BASE COUNT          333 a          229 c          312 g          299 t
ORIGIN      
        1 ccgctattgc cagtacacct cacccgtcct ggcgacgtat ctgtaagaca ctcataaaga
       61 atgatttctg gtgccggact ctctccttca gtccgaacaa accccggcac tatgaacgtt
      121 acctgcagcg tatgaaggaa aggaggaagg aatgggggac tctgtgacac cggagatact
      181 tggcaacatg attcgtcagt atttcagtca ggtacgaact gaaaaagaga gcatacaggc
      241 tctgaatcat ctgcgcagag tgcttcatga ggtcagtcct tttgcacagg agcctgtgga
      301 ctgtgtgttg tgggtgaaag cggatgaggt ggtggccaac gattacaacc ctaatgtcat
      361 ggcactaggt gagaaaaaac tactgaaaca gtctctggag aaagatggtt tcacacaacc
      421 ggtggtggtg tcagaggaga agaatcatta tctggtggtg gatggttttc atcgtcagtt
      481 actggggcgg gaagcagatg caggaaaacg cctgaggggc tggttgcctg tagcctgcat
      541 taatccggag agaaagggac aggccgcacg tattgccgcg accattcgac ataaccgggc
      601 cagggggaaa caccagatca ctttaatgtc tgatattgtc agggatcttt cacgtctggg
      661 ctggacgaat gaacgtattg gtacagagct gggaatggac caggatgaag tgctgaggct
      721 gaaacagatt agcgggctga cagagttgtt tcaggaagag gatttcagct cggcttggac
      781 ggttcgttga tgttgttaaa tatgggcggt atttctgaaa gttgatactt tgggtagaaa
      841 aaatgatacc gaaaactatg gaggatgtgc ttgtcaggtg actggacacc ataggtctta
      901 ggctttgata acgggataag atgagcggct tttctcatac catcaggtgt gatgatggcg
      961 cagatttaca aaatctgatt gattctaaag atgtataagt aaaaatcaag ctttgatatg
     1021 aaaaaacaga tatttattaa taataaacct cctgtagtcc cttactcagg gacgcacgcg
     1081 aaaatattta aatacattga aatccctctg ccgtttttct actttatcta tacatcaggc
     1141 gaaccattcc acatatctgt ccaaaatact gtt
//
LOCUS       DAZPCS010000381         1160 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZPCS010000381 DAZPCS010000000
VERSION     DAZPCS010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.389"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            115..>1160
                     /gene="cadB"
                     /locus_tag="ACHUQ7_004293"
     CDS             115..>1160
                     /gene="cadB"
                     /locus_tag="ACHUQ7_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFR1137388.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKAS"
BASE COUNT          247 a          255 c          298 g          360 t
ORIGIN      
        1 tttaggatta atcctttttt cgtgagtaat cttatcgcca gtttggtctg gtcaggaaat
       61 agttatacat catgacccgg actccaaatt caaaaatgaa attaggagaa gagcatgagt
      121 tctgccaaga agatcgggct atttgcctgt accggtgttg ttgccggtaa tatgatgggg
      181 agcggtattg cattattacc tgcgaaccta gcaagtatcg gtggtattgc tatctggggt
      241 tggattatct ctattattgg tgcaatgtcg ctggcgtatg tatatgcccg actggcaaca
      301 aaaaacccgc aacaaggtgg cccaattgct tatgccggag aaatttcccc tgcatttggt
      361 tttcagacag gtgttcttta ttaccatgct aactggattg gtaacctggc gattggtatt
      421 accgctgtat cttatctttc caccttcttc ccagtattaa atgatcctgt tccggcgggt
      481 atcgcctgta ttgctatcgt ctgggtattt acctttgtaa atatgctcgg cggtacctgg
      541 gtaagccgtt taaccactat tggtctggtg ctggttctta ttcctgtggt gatgactgct
      601 attgttggct ggcattggtt tgatgcggca acttatgcag ctaactggaa tactgcggat
      661 accactgatg gtcatgcgat cattaaaagt attctgctct gcctgtgggc cttcgtgggt
      721 gttgaatccg cagcagtaag tactggtatg gttaaaaacc cgaaacgtac cgttccgctg
      781 gcaaccatgc tgggtactgg tttagcaggt attgtttata tcgctgcgac tcaggtgctt
      841 tccggtatgt atccgtcttc tgtaatggcg gcttccggtg ctccgtttgc aatcagtgct
      901 tcaactatcc tcggtaactg ggctgcgccg ctggtttctg cattcaccgc ctttgcgtgc
      961 ctgacttctc tgggctcctg gatgatgttg gtaggccagg caggtgtacg tgccgctaac
     1021 gacggtaact tcccgaaagt ttatggtgaa gtcgacagca acggtattcc gaaaaaaggt
     1081 ctgctgctgg ctgcagtgaa aatgactgcc ctgatgatcc tcatcactct gatgaactct
     1141 gccggtggta aagcatctga
//
LOCUS       DAZPCS010000382         1140 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZPCS010000382 DAZPCS010000000
VERSION     DAZPCS010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.390"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..730
                     /locus_tag="ACHUQ7_004294"
     CDS             <1..730
                     /locus_tag="ACHUQ7_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HFR1137389.1"
                     /translation="TDRIHLKDAARMLEVSVMTIRRDLHQEDEPLPLTLLGGYIVMVN
                     KPAPSMPVIHDVPKNHRDDLPIAILAAGMVNENDLIFFDNGQEIPLVISMIPDAITFT
                     GICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNSSPLDSLNPRKIFISASGV
                     HNHFGVSWFNPEDLATKRKVMNRGLRKILLARHALFDEVASASLAPISAFDVLISDRP
                     LPADYVTHCQNGSVKIITPDSEDE"
     gene            complement(732..958)
                     /locus_tag="ACHUQ7_004295"
                     /pseudo
     CDS             complement(732..958)
                     /locus_tag="ACHUQ7_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013364090.1"
                     /note="with IlvB catalyzes the formation of 2-acetolactate
                     from pyruvate, the small subunit is required for full
                     activity and valine sensitivity; E.coli produces 3
                     isoenzymes of acetolactate synthase which differ in
                     specificity to substrates, valine sensitivity and affinity
                     for cofactors; also known as acetolactate synthase small
                     subunit; frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
BASE COUNT          298 a          283 c          247 g          312 t
ORIGIN      
        1 aaccgacaga atccatttga aagacgcagc gcgaatgctg gaagtttctg taatgactat
       61 tcgtcgcgat ctccatcagg aagatgaacc tctgccactg accctactgg gtggctatat
      121 tgtaatggtg aataaacccg cgccatccat gccagtaatc catgacgttc caaaaaatca
      181 tcgtgatgac ttacctattg caattctggc tgccggaatg gttaatgaaa atgatctgat
      241 cttctttgat aatggccagg agataccact cgttataagc atgatcccgg atgcaatcac
      301 cttcaccggc atctgttact cacatcgcgt ctttgttgcg ttgaatgaaa agcctaatgt
      361 aacagcaata ctttgtggtg gtacgtatcg tgccagaagt gatgcttttt acgatgccag
      421 taactcttcg ccattagact ctctcaatcc gcgaaaaata tttatttccg ccagcggtgt
      481 gcataatcac tttggcgtca gctggtttaa ccctgaagat cttgccacta agcgtaaagt
      541 gatgaaccgt ggactacgga aaattttgct cgcccgccac gcgttgttcg atgaagtggc
      601 ctctgccagc ctcgcaccga tctctgcatt tgacgttctg attagcgatc gtccgttacc
      661 ggcagattat gttacgcact gccagaatgg ttctgtaaag atcattacac ctgattcaga
      721 agacgaatga cttactgaaa aaacaccaca atcttgttaa acatcgtcgg gttggactga
      781 ttacgttgca ctttcaccac atattccagc ttatctattt ggcttatcac ctactccaga
      841 cgctggtcat ccttgaccag tagccagata tggcttttgt cgcagtcctg aatcggcagg
      901 cagagaatgt cttcaacgtt aagagcgcga cgggcaaaag cccacaaagg tgagtcatta
      961 cacccggatg gtaaacaggc acttccttgt tgtagaagcc gttatattgt ccataatgtc
     1021 tgggttttta atgcatactg ggtaacaacc ggacgaatct tcgaatatca ctggcgctgc
     1081 cagccggttt gaattcatct cacaaccctg catagagcga atctcctgcc tgcacgtcac
//
LOCUS       DAZPCS010000383         1115 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZPCS010000383 DAZPCS010000000
VERSION     DAZPCS010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.391"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(151..633)
                     /locus_tag="ACHUQ7_004296"
     CDS             complement(151..633)
                     /locus_tag="ACHUQ7_004296"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR1137390.1"
                     /translation="MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNAS
                     RTFVICLEDTLRIVGYYSLATGSVTHAELGRSLRHNMPNPVPVVLLGRLAVDVCTQGH
                     GFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV
                     "
     gene            complement(624..926)
                     /locus_tag="ACHUQ7_004297"
     CDS             complement(624..926)
                     /locus_tag="ACHUQ7_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFR1137391.1"
                     /translation="MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRT
                     DFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLEAPVQNAEGRERLMAVKPEWK"
BASE COUNT          325 a          269 c          229 g          292 t
ORIGIN      
        1 ctgatcgaaa gctgattttc aggaaccgtt ttgacggctt cggctttaaa ctccgtagag
       61 taacgttggg tttttctgct cattattggc tccttctgat gccattctat ttcaggaagg
      121 agtgtccgtt aaactcaggc tacctcagac tcagacttta taaaacaagg tattaggtgc
      181 cacaactgac tgaacaaaac caaaacgctc ataaaatgct ctggcgtcat catcaattgc
      241 atgtaccata acagccttaa tgccaacctg atcagccaga ttgacaaccc ggtgaatggc
      301 atcacttagt agccatttac cgaaaccatg cccctgggta caaacatcaa cagccagacg
      361 ccctaacaga acaacaggga caggattggg catgttatgc cgcaaactgc gaccaagctc
      421 ggcatgagtg acggaaccag tagctaatga gtagtatccc acaatacgta aagtatcctc
      481 caagcaaata acgaacgtgc gagaagcatt gagcatctgg tttttcatag cccgaccacg
      541 taaccagtta cttagcacct catttccaca gtcaaatggc tgaagtatat gctcatccgt
      601 caacaatgct ggcgcggtga catttatttc cattcaggct ttaccgccat cagacgttca
      661 cgcccttctg cattctgcac aggagcttcc agttgcgtaa taaacgaatc ataacgttcg
      721 ttatccagca caaataaacg ttgatccagg ataatgtttt tagcttcctg gtacgccagt
      781 tccatgatga aatcggtacg gttcttattg accaagctcg cagcataatc aataacggct
      841 cgttcctcat cagttgcccg gatgttaatc tgatgttctc gcggtgtttt acggacgctt
      901 gcagctgtag acataaaaac ctccattggt aacaatttgc tatgcaatta atgttatcgc
      961 aaatggtaac agaacgctat acagaatcac ttacgggaaa agcgatcccc tgataaaaac
     1021 aatggcagag acctgaacat tgtgacgatg atctgtgtcg gcgcacaaat catgtctgat
     1081 tacggccgtt taatgtgatt ttgataatga aatgc
//
LOCUS       DAZPCS010000384         1109 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZPCS010000384 DAZPCS010000000
VERSION     DAZPCS010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.392"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            1..1019
                     /locus_tag="ACHUQ7_004298"
                     /pseudo
     CDS             1..1019
                     /locus_tag="ACHUQ7_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            992..>1109
                     /locus_tag="ACHUQ7_004299"
     CDS             992..>1109
                     /locus_tag="ACHUQ7_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1137392.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQV"
BASE COUNT          312 a          271 c          258 g          268 t
ORIGIN      
        1 aattctcttg caccattaac tatgttcagt ccctgcgcga gttgtacacc gatgtcatcg
       61 aagagtttta ctgggttgcg ctcccgctta cgacgcaaaa ttccctttca caataccaac
      121 ccgaatggca gtgctgggaa cctgatgtcg aatgggtgcg tcagcccccg caagatgcga
      181 taaccgctcc tgactttttc tgcttttacc agccaggcat gaccttcgaa caatttgtac
      241 gtgagtttgc cgaatggttt tcacaaaaac gtccggcggc gatgatgatc ggcatccgtg
      301 cggatgagtc ctacaaccgt tttgtcgcca tcgccagttt aaataaacaa cgttttgccg
      361 acgataaacc atggactacg gccgcaccag gcggtcatag ctggtacatt taccccattt
      421 acgactggaa agtggctgat atctggacct gatatgcaaa tcatcaaaat ctctgcaacc
      481 cactgtataa cctaatgtat caggcaggcg ttcctctgcg tcatatgcga atttgcgaac
      541 cttttggccc ggagcaacga caaggattat ggctctgtca cgttatcgaa ccggatcgct
      601 gggctgctat gtgcgcacga gtcagcgggg taaaaagtgg cggcatttac gccggacatg
      661 acaatcattt ctatgggcat cggaaaatcc tcaaaccaga acatttagac tggcaagaat
      721 atgcattatt gctgctcaat agcatgccgg aaaaaacagc tgagcattac cgcaataaaa
      781 tcgccattta tttgcactgg tatcagaaaa aaggcatcga ggttccacaa acccagcaag
      841 gggacattgg cgcgaaagat atcccctcct ggcggcggat atgcaaagtt ttactcaata
      901 acgattactg gtgtcgggca ttatcattta gtcctacgaa agcgaagaac tatcagcgtt
      961 ataacgaacg gataaaagga aaacgtcagg aatgggggat actatgcaac aacgattaac
     1021 gcaggattta acgcaatttc tcgccagctt gccggaagat gatcgcatca aagcgattaa
     1081 tgaaatccgc atggcgatcc atcaggtta
//
LOCUS       DAZPCS010000385         1091 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZPCS010000385 DAZPCS010000000
VERSION     DAZPCS010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.393"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(20..823)
                     /gene="dkgB"
                     /locus_tag="ACHUQ7_004300"
     CDS             complement(20..823)
                     /gene="dkgB"
                     /locus_tag="ACHUQ7_004300"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFR1137393.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKVIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(986..1062)
                     /locus_tag="ACHUQ7_004301"
     tRNA            complement(986..1062)
                     /locus_tag="ACHUQ7_004301"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1026..1028),aa:Asp,seq:gtc)
BASE COUNT          285 a          255 c          248 g          303 t
ORIGIN      
        1 gaggagctgt cagagaggct taatcccatt caggagccag accttccggg ctaaccaggc
       61 ggtcgttgca atccagtgcg gcgatcgctt ttttatcttc ggcatcaagc tgtaaatttt
      121 gtgccttaag attactttcc aggtttttac gtttagtaga agaaggaatt actgagtaac
      181 cttcccccat agcccacgcc agaatcactt gtgccggaat cgcattgtgt ttagctgcaa
      241 tacgagcaat aacctcatct ttcagtgcct taccataagc cagcgtcata taggaagtga
      301 tatgaatacc gtgctgttta gcccaggcaa ccactttacg gttttgcaga taaggagaga
      361 gttcaatctg gttggtagcg atgttttcag cgccaacagc agcaatcact ttttccatca
      421 atgggatcgt gaagttggaa ataccgatct cacgcgtcag cccttgtttt ttggcttcca
      481 gcagcgcctg cataaactct tcaacagaga cttcatcgtt tggtgacggc cagtggatta
      541 gcgtcagatc aacataatcg gtacgcaatt tttgcaggct ctctttcaga ctcgggatca
      601 atttgtcttt gctgagattt tcaatccaga ttttagtggt gatgtagagt tcatgacgtg
      661 gcacgccact ttctgcaatc gcctgaccta ctgcggcttc gttatcatag atttgtgcgg
      721 tatcaattgc gcgataacca agttcaagcg ccgttttcac agatgaaata acaacgtcgt
      781 ctttcagacg gaaagtacct aaaccaaatg cagggatagc cataatatgc ctcttttaat
      841 gctattttcg ttaactgagg tgattatggg cttcagactg ttgcgggaaa agagcgtaaa
      901 acgcagagga tttttgcgat tctagcaata atagatatcc agacgcaaaa aaacatcgag
      961 cgttaactcg aggcttctta ataagtggcg gaacggacgg gactcgaacc cgcgaccccc
     1021 tgcgtgacag gcaggtattc taaccgactg aactaccgct ccaccgaatt cttttacaac
     1081 caccggtttt a
//
LOCUS       DAZPCS010000386         1085 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZPCS010000386 DAZPCS010000000
VERSION     DAZPCS010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.394"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            30..209
                     /locus_tag="ACHUQ7_004302"
                     /pseudo
     CDS             30..209
                     /locus_tag="ACHUQ7_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(226..916)
                     /locus_tag="ACHUQ7_004303"
                     /pseudo
     CDS             complement(226..916)
                     /locus_tag="ACHUQ7_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            995..1085
                     /locus_tag="ACHUQ7_004304"
                     /pseudo
     CDS             995..1085
                     /locus_tag="ACHUQ7_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          288 a          247 c          282 g          268 t
ORIGIN      
        1 aattactgta aaaaaacgat acacatttaa tacgagggag gccggcagaa aaacggcttg
       61 cggtcaggca ggagaaatgt gtgccgcgaa tgaagtcctt gtatgactgg ataaagctcc
      121 agaggaaaac tttgtcaaac catgctgaaa tggtaaaggc gttcaactat atcctgaatc
      181 actggaatgc actgaatgag ttttgccggg tagtacttcc aatcattaga atatgtgctt
      241 ttcaatgaag gaaccgatga ctttttcgtg gatttccacg gaacgcgaga agcagattgt
      301 tttacgtgcc agccgcttga tgcgggggcg gagggtcagg ttgttacgtt catacgttgc
      361 gtgaagattt tgccggtcag gtgtttctcc tccggcactt ctctggcgta gctgccccag
      421 tcatcgctgg tcaccatgcc gatacagaac ggagtcaaca gggctaacag ttcccgacag
      481 gtctcatcat tccgtggccc gcaagtgtaa gccaacacac cgccagtttt agtgttgtac
      541 gcataccagg gccagtgttg ttgcgccttg ctgccaacga agcgccactg ctcatcaagt
      601 tcacagataa gggcaacatc agcgtgagcg actggcgcag atgttatccg ccggggcgag
      661 agctttttaa tgtccgtatg acggtattga tgccgatttt cagtgtcctg gcggtatcac
      721 ggccccctgc gccgttatgt gccatttcaa cgatctgctc cttaacgccg agttttctgg
      781 cttcataaga gtaagtgagc tggaaaacac gtttacatga gcgacaacgg aaacgctcat
      841 gccgggaaca actacggcca tgtcgataaa cttcatcgga attacagcgc ggacaatgaa
      901 cagtaacggt agtcatggaa aacctcaaat accagtagat taccagaaac ttctatcgat
      961 tgaagacatt accttttgcc gcaataggtg gggtaacatt ggtgaaaacg cattacgccg
     1021 ggtggcagtt ggaaaaaaat tatctttttt cggctctgat cgtggtggag aaaatgcggc
     1081 aataa
//
LOCUS       DAZPCS010000387         1084 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZPCS010000387 DAZPCS010000000
VERSION     DAZPCS010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.395"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(108..479)
                     /locus_tag="ACHUQ7_004305"
     CDS             complement(108..479)
                     /locus_tag="ACHUQ7_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1137394.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            complement(540..>1084)
                     /locus_tag="ACHUQ7_004306"
     CDS             complement(540..>1084)
                     /locus_tag="ACHUQ7_004306"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1137395.1"
                     /translation="FAARLNSSVRELGKTMRDLLKVVLSLVLAMASGQALADLPIVLV
                     DEARLPYDYSPSNYDISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRR
                     LIYSANGSRTFAGYYVIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVI
                     NGQFSLALTDNGLKIMYLSN"
BASE COUNT          256 a          282 c          259 g          287 t
ORIGIN      
        1 cataccttgg cctaggtaaa gaagatcaga ctcgtagcca catcggtggc aactgagctt
       61 gtacgcaatg cccatctggt tgtctcacct gaatcgtaac gtcagagcta ttcatccatc
      121 cattcggcat caatgtatgc cactaagccg accagctgac cgtttggact gaagtatgtt
      181 gcctgcaccc cacttgacat ctcatcaata ccaccgcact gcgctaggcg ccggtcccac
      241 ttgcccacag aagcgtcagg atcggctgag ttccccaaga cccttgtgag ccatgcctcc
      301 tttaactcaa cctctcgaat gattgcgtcg gccagctcct ccgcgttatc tagttcgaca
      361 tagtctggaa ttcggacgta cgttctgaac acagctggat acttacttgg gcgattgtca
      421 tacatgacat caacaatgta cccgtttgtg taaccgtctc ttggaggttc gtatgacact
      481 agtggttccc ctcagcttgc gactagatgt tgaggcctaa cattttatta gagagcaggc
      541 tagttgctta gatacatgat cttcaggccg ttatctgtca gggcaagcga aaattggcca
      601 tttatgacga ccaatgcccc gcagaagctc ccatctttgc cgccatagac gccgcgcccc
      661 ccttttgggg tgtagaacat ccttttgcca gatgtggaaa agaagttcgt tgtcccattg
      721 ttggcaatga cgtagtagcc ggcgaaagtg cgagacccat ttgcgctata tataagccta
      781 cgatttccgt tgcgactatt gtcgtaattg gatgaactat tatcgtagtt gctctcagag
      841 ttgtcgtaat ttgatggact attgtcgtaa ttgcttatgg agttgtcgta gttgcttgga
      901 gaaatgtcgt agttggatgg ggagtagtca tagggaagac gagcttcatc cactaaaaca
      961 attggcaggt cagcaagtgc ctgccccgat gccatcgcaa gtacgaggct tagaaccacc
     1021 ttcaacagat cgcgcatagt cttccccagc tctctaacgc ttgagttaag ccgcgccgcg
     1081 aagc
//
LOCUS       DAZPCS010000388         1083 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZPCS010000388 DAZPCS010000000
VERSION     DAZPCS010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.396"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(357..1052)
                     /gene="aqpZ"
                     /locus_tag="ACHUQ7_004307"
     CDS             complement(357..1052)
                     /gene="aqpZ"
                     /locus_tag="ACHUQ7_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HFR1137396.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
BASE COUNT          304 a          324 c          255 g          200 t
ORIGIN      
        1 cagtctaggg atttttaatc ctatctgagt gtttagcatt gttgcgctcc tttcatgtgc
       61 aatgccatat aaagccactg gcggagggag cgcgcagggg gcagccagtc gccgccgccc
      121 cctgcacccc cgggctctgg cgaacaaaat cgccgctgcg cggtaccttc agcttatccc
      181 cttcggcaac cgggtcgggc gcgaggtaac gtcctgtaag acgcgccctc agcccacatc
      241 catgtgggct gccccggcct tcggggaacg cttcagcgat tttgacgcca ccaatacccg
      301 tgcggtttgt aatgtcgaag ataaaacaac cagggcagat aaaatgccaa tgctaattaa
      361 tcacgctttt ccagcagagt ccggtaaatc agaccaccga taatgccgcc gacaattggc
      421 accacccaga agaaccacag ttgttctaat gcccagccgc cctggaagat agcaaccgcg
      481 gtgctgcgcg ccgggttaac agaagtgtta gtcaccggaa tactgattaa gtgaatcagg
      541 gttaaggcca gaccaatagc gatcggcgca aaacctgccg gagcgaattt gtcggttgca
      601 ccgtggatca ctaacaggaa gcccgcactc aataccagtt caactaccag cgcggaaagc
      661 atggaataac cgcctggtga atgctcgcca taaccgttag aagcaaaacc gctggctgcc
      721 gcgtcaaaac ccgttttacc actggcaatt aaatacagca gcgccgctgc aacaataccg
      781 ccgacaacct gggcaattac gtagccaacg acttcttttg ccggaaaacg tccgccagcc
      841 cataaaccaa tagtgaccgc cgggttaaaa tgaccaccag aaatatgacc aacagcgaag
      901 gccatcgtca gaacggtcaa accaaatgcc agcgccacgc cggcaaaacc aatgcctaat
      961 tccgggaagc ctgcggccag tacagcacta ccacagccac caaaaacaag ccagaaagta
     1021 ccaaaacatt cagctgctaa ttttctgaac atatccacct caattaaaaa ttgaccctgt
     1081 gaa
//
LOCUS       DAZPCS010000389         1069 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZPCS010000389 DAZPCS010000000
VERSION     DAZPCS010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.397"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(227..652)
                     /locus_tag="ACHUQ7_004308"
     CDS             complement(227..652)
                     /locus_tag="ACHUQ7_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFR1137397.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRQCFCLSCHGRLILQTD
                     AQGAWFEHDLHALSEQQKADCVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCEQFF
                     HGKKFCEACGTGIYCRAVCTKSVYSYQPDLFEDCGGAST"
     gene            complement(649..1032)
                     /locus_tag="ACHUQ7_004309"
     CDS             complement(649..1032)
                     /locus_tag="ACHUQ7_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFR1137398.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYDSYTCHLCGSALRYHPQY
                     DTERPWFEHADEGLTEHGHECPYVRPERREIRLIKRLQQFVPDTLPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGRFSEEGGAE"
BASE COUNT          253 a          308 c          265 g          243 t
ORIGIN      
        1 ctcgcgtatg gttgattgac accacagtgc gaattcggcg aatgcgagat cacggtacga
       61 ttccgcacca cactaccaat caataccccg ggttttattc aggtaagcag gactgcggat
      121 atccggtgtc gcgcctttgt gtcacgaacg gggtgggtgc gaaacaccag ataaaataca
      181 ggttggcaat acctctgaac gccctgagca ggtcgggcat ttcggattaa gtactcgcac
      241 ctccgcagtc ctcaaacaag tctggctggt agctgtaaac agacttcgta cataccgctc
      301 tgcaataaat ccccgtacca caggcctcac agaacttctt cccatgaaaa aactgctcac
      361 acatcacaca atgccactcc agcacgaccg gtaatggcga taaaaacatc gccatatcct
      421 caatgtaagg gtgggacttc tccggattca gcaccacaca gtccgccttc tgttgctcac
      481 tcagggcatg taaatcatgc tcaaaccacg ccccctgagc atctgtctgc agaatcaacc
      541 ggccatggca ggaaaggcag aaacactgcc ggatatttct gctgagcgca gagtcggcac
      601 tgataatgtg ctcacccggc gtgatcccca gccccgtttt tataccgttc attctgctcc
      661 cccctcctca ctgaagcgcc cggtctggca gtgtgtgcag tactgctccc cataataatc
      721 gtggtgacat tgcctgcagt gccagctggc tttacgtact acgggtaagg tatccgggac
      781 gaattgctgc agacgtttaa tcaaccgtat ttctctgcgt tccggcctga cataagggca
      841 ctcatgaccg tgctccgtca gcccttcgtc agcgtgttca aaccagggcc gttcagtgtc
      901 gtattgcggg tggtatctga gtgcactgcc gcaaaggtgg caggtgtagc tatcgtaagg
      961 tgcagtctgt gcggtacggg caccggtcag acgtccgttg ccatcaagtg cgagaaaaga
     1021 ttttgcgtac atagtatatg ttcctcagcc gccagacgac acgcaggca
//
LOCUS       DAZPCS010000390         1068 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZPCS010000390 DAZPCS010000000
VERSION     DAZPCS010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.398"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..560
                     /locus_tag="ACHUQ7_004310"
     CDS             <1..560
                     /locus_tag="ACHUQ7_004310"
                     /inference="COORDINATES: protein motif:HMM:NF024120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HFR1137399.1"
                     /translation="TKGDAMIGVKQPLPKAVLRTQHDKNKEAISILDFGVIDDGVTDN
                     YQAIQNAIDAVASLPSGGELFIPASNQAVGYIVGSTLLIPGGVNIRGVGKASQLRAKS
                     GLTGSVLRLSYDSDTIGRYLRNIRVTGNNTCNGIDTNITAEDSVIRQVYGWVFDNVMV
                     NEVETALFNARALALKIYSMSGWNL"
     gene            787..>1068
                     /locus_tag="ACHUQ7_004311"
     CDS             787..>1068
                     /locus_tag="ACHUQ7_004311"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endorhamnosidase"
                     /protein_id="HFR1137400.1"
                     /translation="MEQLDLDYCGSTGVVIENVNGGFSFSNSWIAADADGTEQFTGIY
                     FRTPASTQSHKIVSGVHINTANKNTAANNQSIAIEQSAIFVFVSGCTLTG"
BASE COUNT          314 a          202 c          257 g          295 t
ORIGIN      
        1 acaccaaagg cgatgccatg attggcgtca agcagccttt accaaaagca gttttaagaa
       61 ctcagcatga caaaaataaa gaagcaataa gtatcctgga ttttggtgtt attgatgatg
      121 gtgtgacaga taattaccag gcaatacaaa atgctataga tgccgttgct tcactaccct
      181 ccggcgggga gctgtttatc cctgcgagca accaagcggt ggggtatatt gttggatcca
      241 ctttgcttat tcctggcggt gttaacatca gaggagttgg taaggcatcg caactccgag
      301 caaaaagcgg acttacagga tctgtgttaa ggctgtctta tgattcagac actatcggcc
      361 gttatctgag aaatatacga gtaactggta ataacacctg caatggtatt gacacaaaca
      421 ttacagcaga agactctgtc atcagacagg tttatggctg ggtatttgat aatgtaatgg
      481 tgaatgaagt tgaaaccgca ttatttaatg caagggctct ggcactcaaa atttatagca
      541 tgtcaggctg gaacctgtag agtcggtctt cactttttag gccagtgcgt aagtgttagt
      601 gtcagctcct gccatttcag cagaggaaat tattctgctg atgaaagctt tggcatcagg
      661 attcagcctc aaacgtatgc gtggtcgtca gaggcagtta ggtcagaagc aataatttta
      721 gacagtgaga ccatgtgcat tggttttaaa aatgccgtct atgttcatga ttgccttgat
      781 ttgcatatgg aacaactgga tttagattat tgcggctcaa caggcgtggt tatagagaat
      841 gtaaacggag gattttcttt ctcaaactca tggatagcag cagatgccga tggcactgaa
      901 caatttacgg ggatatattt tagaacgccg gcctcaacgc agtcacataa aattgtcagt
      961 ggtgttcata tcaacactgc aaacaaaaac acggctgcaa acaatcaaag tatagcgata
     1021 gaacagtcgg cgatcttcgt ctttgtaagt ggttgtacgt taactggt
//
LOCUS       DAZPCS010000391         1066 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZPCS010000391 DAZPCS010000000
VERSION     DAZPCS010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.399"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(570..749)
                     /locus_tag="ACHUQ7_004312"
                     /pseudo
     CDS             complement(570..749)
                     /locus_tag="ACHUQ7_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            843..>1066
                     /locus_tag="ACHUQ7_004313"
     CDS             843..>1066
                     /locus_tag="ACHUQ7_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR1137401.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVVPASELAA"
BASE COUNT          285 a          193 c          277 g          311 t
ORIGIN      
        1 ttagggtagt cactatattt gcgactgagc tggtcgctat ttttaaccag gctgaaaaat
       61 tgagtcaata tatcgaaagc ggtagttggc gggcataagt atgtaatgat gatgctgcac
      121 tggtgttgaa tattattcac gccaaacttt cctcattgag caatgtcgtc gcaagtggcc
      181 tcaatgattt tagaccatta tcagaggtcc ggagcggcgg gaagagattt attgctgtgg
      241 aataattgtg gtactgatga ttatttatat cccggattgg tagggaaaag cttttttcat
      301 gcactatttt tgcaaatatt tctatatggg aagtggtttt gttacagatt aaatttataa
      361 agaaatgcat ttggggtgtt gggttgaaga gtagctggta gatgttattg tgccagcagg
      421 ggcaggggaa tgtgccgtat cgatatgctt tggcaattgc gtgtgccgca gatggagatg
      481 aaatgccaat aaaaaagcct gagttcgatg agggacgaac tcaggctggt gttatgtttt
      541 acagtaacac gcctcggcgc gttggctgat tagaactggt agacgatacc aactgccagt
      601 tggtcgtcgg tgttcagtcc atgagcgaga acataatcac tcttatcaag caggttgatc
      661 tgataagcgg tatatatgtt catattctta ttgaagtaat accaggtacc gatttcaata
      721 tagttcagac gatcagaatc tttgtaacct gtagactggc cccctgaatc tccagacaac
      781 caatatcact tatttaagtg atagtcttaa tactagtttt tagactagtc attggagagc
      841 agatgattga tgtcttagga ccggagaaac gcagacggcg tactacacag gaaaagatcg
      901 ctattgttca gcagagtttt gaaccgggaa tgacggtctc ccttgttgcc cggcaacacg
      961 gtgtggcagc cagccagtta tttctctggc gcaagcaata ccaggaggga agtcttactg
     1021 ctgtggccgc aggagaacag gtcgttcctg cctctgaact tgctgc
//
LOCUS       DAZPCS010000392         1047 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZPCS010000392 DAZPCS010000000
VERSION     DAZPCS010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.400"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..318
                     /locus_tag="ACHUQ7_004314"
     CDS             <1..318
                     /locus_tag="ACHUQ7_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5655 domain-containing protein"
                     /protein_id="HFR1137402.1"
                     /translation="LFDHLRDEVMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLR
                     LSLNMQFHELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMES
                     ALV"
     gene            complement(365..706)
                     /gene="symE"
                     /locus_tag="ACHUQ7_004315"
     CDS             complement(365..706)
                     /gene="symE"
                     /locus_tag="ACHUQ7_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HFR1137403.1"
                     /translation="MTDTHSIAQPLEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELMQSLRKVCKLSARKQRQVQEF
                     IGVITGKQKVA"
     gene            complement(927..>1047)
                     /locus_tag="ACHUQ7_004316"
     CDS             complement(927..>1047)
                     /locus_tag="ACHUQ7_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1137404.1"
                     /translation="IFDKINSIEAVCNGLISYIGIYHKTQLHLADALTDAAIN"
BASE COUNT          280 a          209 c          263 g          295 t
ORIGIN      
        1 ttattcgatc atcttcgcga tgaagttatg cgcctggacg cagggatcac gcaggaagta
       61 ttgaagctat atattgcgtt taaagcggaa acgaattttg ttgatgttgt gccgcaaaaa
      121 agccgactgc gattgtcgct taatatgcag tttcatgaac tggtcgatcc gaaaggtatt
      181 gccaaagatg tgacaaatgt tgggcgctgg ggtaatggcg atgtagaaat tggtttcagc
      241 gacctcgcac aacttcctta cattatggga ttaattcgtc aggcatttga aaaacagatg
      301 gagagcgcgt tggtataacg cggcgaataa tacccggtag cactggcgct accgggtcaa
      361 tgtatcaggc gactttctgt ttacctgtaa ttaccccaat aaattcctgt acctgccttt
      421 gtttacgcgc cgacagctta cacaccttgc gtagtgactg catcagttcg ctctcttcgg
      481 cggcggcggg ttgggcggtg aggacaatac agccttccat cactttgaca tctaccgccg
      541 tgccggtggc aaaaccggcg gcttccagcc actgaccttt cagggtgatg gcgggaatac
      601 ggctgtaatc cgggtaacga ctcgcataac tcacggttaa ttgacgatta tttgccgggg
      661 agacttctgc ttctaacggt tgtgcaatag aatgcgtgtc agtcatgatt actattctct
      721 ataaatagtg attgtgatta gcggtgcgag tgtgttggcg cacatccgca ccgtgctaaa
      781 tacctggata catcatcagt aaatacaggg aaagtccagc taaaaattaa tgaaaataag
      841 atgaaaatta gcgtgaaata taaattttgg tgatgtgaat aaagcggcta tttagccgct
      901 ttattggttt tgtgagggcg agggaattag tttatcgctg cgtcagtgag tgcgtctgca
      961 agatgcagtt gggttttatg ataaatccca atataagaaa taagaccatt gcatactgct
     1021 tcaatagaat ttatcttatc aaatatt
//
LOCUS       DAZPCS010000393         1034 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZPCS010000393 DAZPCS010000000
VERSION     DAZPCS010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.401"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(2..133)
                     /locus_tag="ACHUQ7_004317"
     CDS             complement(2..133)
                     /locus_tag="ACHUQ7_004317"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1137405.1"
                     /translation="MARSAKSRKRKPASQRSKLPRYVVKLHEDDFFDEEDAEVLRFD"
     gene            215..408
                     /locus_tag="ACHUQ7_004318"
                     /pseudo
     CDS             215..408
                     /locus_tag="ACHUQ7_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748652.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
     gene            551..772
                     /locus_tag="ACHUQ7_004319"
                     /pseudo
     CDS             551..772
                     /locus_tag="ACHUQ7_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(793..855)
                     /locus_tag="ACHUQ7_004320"
                     /pseudo
     CDS             complement(793..855)
                     /locus_tag="ACHUQ7_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          312 a          245 c          237 g          240 t
ORIGIN      
        1 ttcaatcaaa gcgcagaact tctgcgtctt cttcgtcaaa aaaatcatct tcgtgaagct
       61 tcacgacata gcggggaagt ttgcttcttt gagaggcggg tttacgttta cgggatttag
      121 ctgaacgggc catataacca cctgaaagac aatgacattg cctgttttta taacggtaat
      181 tgcagaccat gacaaaccgc agccgtcagg ctgcctactc gagtcacaag gctctttgtg
      241 tccgatatgc cggcagcgca ttacccatga aactggctgg aacgtacacc ataaggtgaa
      301 gaaagttatg ggggcggtga agaattatct aaccttgtct tgttacatcc aaattgccat
      361 cgacagttac acagttgtga agccggctct ctcaaaaagg gcttataaag gcttgagcgg
      421 tgtaccggga aaccggtatg caccgttctt aggggagggc agtccagcaa tgaactgtcc
      481 ttacccgaca aacatccaaa acgaacggaa cgtgctggaa tccgcatata acccgttgta
      541 aagtgaggaa atggacacct cacttgctca tgagaacgcc cgcctgcggg cactgttgca
      601 gacgcaacag gacactatcc accagatggc tgaatacaac cgcctgctct cacagcggat
      661 ggcggcttat gcttccgaaa tcaaccgact gaaggcgctg gttgcgaaac tgcagcgtat
      721 gcagttcggt aaaagctcag aaaaacttcg cgcaaaaacc gaacggcaga ttccattttc
      781 acgggcgatc tacgccacac aggctctggg ttgcagtgac tgctccatta aggcgatttt
      841 gaactcataa gggaagtaac aaaaaaactg caaaagtaca ataattatat atggccaaac
      901 taacctctat taaaaaacat cagtttttca cacttagttg agcttcatga aaaaatcatc
      961 gaggatttca ttgcagaata cacattccac taactggaag cactccataa gaggaatcag
     1021 agaaagtatc gttt
//
LOCUS       DAZPCS010000394         1017 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZPCS010000394 DAZPCS010000000
VERSION     DAZPCS010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.402"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..238
                     /gene="mxiH"
                     /locus_tag="ACHUQ7_004321"
     CDS             <1..238
                     /gene="mxiH"
                     /locus_tag="ACHUQ7_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system needle filament
                     protein MxiH"
                     /protein_id="HFR1137406.1"
                     /translation="PNDDWTLSSLSETFDDGTQTLQGELTLALDKLAKNPSNPQLLAE
                     YQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            251..544
                     /gene="mxiI"
                     /locus_tag="ACHUQ7_004322"
     CDS             251..544
                     /gene="mxiI"
                     /locus_tag="ACHUQ7_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HFR1137407.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSNPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            550..>1017
                     /gene="mxiJ"
                     /locus_tag="ACHUQ7_004323"
     CDS             550..>1017
                     /gene="mxiJ"
                     /locus_tag="ACHUQ7_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctJ family type III secretion inner membrane
                     ring lipoprotein Mxi"
                     /protein_id="HFR1137408.1"
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSP"
BASE COUNT          347 a          159 c          203 g          308 t
ORIGIN      
        1 accgaatgat gattggacat tgagttcatt atctgaaact tttgatgatg gaactcaaac
       61 attacaaggt gaactaacat tggcactaga taaattagct aaaaatcctt cgaatccaca
      121 gttgctggct gaataccaaa gtaaattatc tgaatataca ttatatagga acgcgcaatc
      181 caatacagtg aaagtgatta aggatgttga tgctgcaatt attcaaaact tcagataata
      241 gggagcattc atgaattaca tttatccagt caatcaggtt gatattatca aagccagtga
      301 ttttcaatct caagagatat caagtctgga agacgtcgtg tcggctaaat atagtgatat
      361 taagatggat acagatattc aagtatcaca aataatggag atggtaagca atccagaatc
      421 attaaaccca gaatctttgg ccaagttaca gacgacgctc tcaaattatt caataggagt
      481 atcattagct ggcacgttag caagaaaaac agtttcggct gttgaaactt tattaaagtc
      541 ttaatttata tgattaggta taaaggtttt attttattct tgttgctgat gttgattgga
      601 tgtgagcaac gtgaagagtt aatttctaat ttatctcaaa gacaggcaaa tgaaataata
      661 tctgtgctag aacgccataa tattactgct agaaaagttg atggaggtaa acaggggatc
      721 tcggtacaag tcgaaaaggg gacatttgca tcggcagttg atttgatgcg catgtacgat
      781 ttgccaaatc cggagagagt tgatatctca caaatgtttc ctacagattc attagtgtct
      841 tctccaagag ctgaaaaggc ccgtttatat agtgctattg agcaacggct ggaacagtct
      901 ttagtttcta ttggtggtgt tatttcggca aaaatacatg ttagctatga tcttgaagaa
      961 aaaaatatat cttcaaaacc gatgcatata tcagtaatcg ctatatatga ctcaccg
//
LOCUS       DAZPCS010000395          990 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZPCS010000395 DAZPCS010000000
VERSION     DAZPCS010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..990
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.403"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            82..>990
                     /locus_tag="ACHUQ7_004324"
     CDS             82..>990
                     /locus_tag="ACHUQ7_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl1 family transposase"
                     /protein_id="HFR1137409.1"
                     /translation="MPVHKVCQNFFRDALAPFHQYRQNALMDATMALINGASLTQTSI
                     GRFLPGNAQVKNKIKRIDRLMGNEALHRDIPMIFRNITSMLTRQLSLCVIAVDWSGYP
                     SQEHHVLRASLLCDGRSIPLLSKVVPSEKQNNPLIQHDFLDSLAQSLPPDARVIIVTD
                     AGFQSAWFHHITSLGWDFIGRIRNNVQYCLDNAPERWLKVSDSPECKTPEYMGAGRLV
                     KERKKSIRGHFYTYKKSAKGRKKKRSKGQSGLNKTDKEQSKSAKEAWLIFSSTNDFRA
                     REIIKLYSRRMQIEQNFRDEKNGRFGF"
BASE COUNT          301 a          206 c          228 g          255 t
ORIGIN      
        1 tgaggaatcc ccagaaaacg agacaaacat agcattatgt gatctactga tttgtgccaa
       61 caattcaatg agatcactgc tatgcctgtt cataaagtat gtcagaattt cttccgggat
      121 gccttagcac catttcatca gtaccgacaa aatgccctga tggatgccac aatggcgtta
      181 atcaacggag catctctgac gcagaccagt atcggacgtt ttttgccggg taatgcacaa
      241 gttaagaata agatcaaacg tattgatcgc ctcatgggta atgaggcgct ccatcgcgat
      301 attccaatga ttttcaggaa tatcacttca atgctgacac ggcagttatc tttatgtgta
      361 atagccgtag actggagtgg ctatccctcc caggagcatc atgttcttcg tgccagcctt
      421 ctgtgtgacg ggcgttccat tccgttattg agtaaggttg ttccttctga aaaacaaaat
      481 aatccattga tacaacatga tttccttgat tcccttgctc aatccctgcc ccccgatgcc
      541 agggttatta ttgtcacgga tgctggattt cagagcgcat ggtttcacca tattacttct
      601 ctgggctggg attttattgg tcggatcaga aataacgttc agtattgcct cgataacgcg
      661 cctgaaagat ggttaaaagt gtcggatagc cctgaatgta aaacaccgga atatatgggg
      721 gctggaaggc tggttaaaga aaggaaaaaa agcatccggg ggcattttta cacctataag
      781 aaatcggcaa aagggcgaaa aaagaaacgt tcgaaaggtc agagtggttt aaacaaaaca
      841 gacaaagaac agagcaaatc agcaaaagaa gcctggttga tcttcagcag tacaaatgac
      901 ttcagagcgc gagaaatcat caagttatac agtcggcgaa tgcagataga gcaaaacttt
      961 cgtgatgaaa agaacgggcg cttcggattt
//
LOCUS       DAZPCS010000396          984 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZPCS010000396 DAZPCS010000000
VERSION     DAZPCS010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..984
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.404"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..107)
                     /locus_tag="ACHUQ7_004325"
     CDS             complement(<1..107)
                     /locus_tag="ACHUQ7_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin"
                     /protein_id="HFR1137410.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGG"
     gene            complement(148..603)
                     /gene="csgB"
                     /locus_tag="ACHUQ7_004326"
     CDS             complement(148..603)
                     /gene="csgB"
                     /locus_tag="ACHUQ7_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFR1137411.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAVVVQRQSQMAIRVTQR"
BASE COUNT          267 a          237 c          176 g          304 t
ORIGIN      
        1 ccgccaccac cgtggttacc gccgccgccg tactgaggaa caacacctgc cagagcgcta
       61 ccggagaata cgattgctgc aattgctgct acttttaaaa gtttcatgta aaacccccat
      121 cggattgatt taaaagtcga atggtaatta acgttgtgtc acgcgaattg ccatttgcga
      181 ctgtctctgc actacaactg ccgttttttg agtaccatac tgtgtaatat ttgctttatt
      241 accagaacct ttctggataa tcatcgcagt attaccataa gcaccttgcg aaatactggc
      301 atcgttggca ctgcccgcct gatcaatata tgcaaggtta taatctcctg tctggtcaat
      361 ctttgcccga ttgctactac cttcttgcgc aacaaccgcc aaaagttttg agcctccctg
      421 ccgtaactga gcactattat tagtcccagc ctgaccaatt atggctgcct gattaaatga
      481 agacttactc aattcattta ccgcgaagtt atattctgaa ttagctaaat cataacctgc
      541 tgcggctgca atcccaggcg cacccagtat tgttaacatc ataaataaca atttgttttt
      601 catgttgtca ccctggacct ggtcgtacat ttaagaaatt aaatcatttc aacttggttg
      661 ttaacgcaac ctgtattttg ttaacgctgc gttacgatgg aaagtatgtc tgcggaaata
      721 tttttaataa ctcacccgcg cgttgtattt ttcttttttt gttcatacca aagtattaaa
      781 acccgcgtaa atacgctgtt atctacgcaa aaaatatttt gttttctctt aaatctccgt
      841 tttccgctat caaaaatcac cagacagtca ttcttcttgc ccgtcgctga ttgctgcgat
      901 atgtcttgcg cacaagccgt gacaaacctc gtctttttgc agtaaaaaaa ttgtccacgg
      961 aggtgtggag aaaaaacaaa aacg
//
LOCUS       DAZPCS010000397          962 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZPCS010000397 DAZPCS010000000
VERSION     DAZPCS010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..962
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.405"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            242..844
                     /gene="fimB"
                     /locus_tag="ACHUQ7_004327"
                     /pseudo
     CDS             242..844
                     /gene="fimB"
                     /locus_tag="ACHUQ7_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
BASE COUNT          291 a          166 c          204 g          301 t
ORIGIN      
        1 ttagattcgt atcagattga taaatatata aaggtacata gcatgcaaga gcatggcgtt
       61 tgtatggcaa agttattata agtaactatc gctactccat ttaagttcgc tcagaagaac
      121 tggtccactt acgtaagtta ttaagcaaac gttcgctttt ataaacataa tcaggataaa
      181 aatgttggat tattgctaac ccagcacagc tagtgcgcgt ctgtaattat aagggaaaac
      241 gatgaggaat aaggctgata acaaaaaaag gaacttcctt acccatagtg aaatcgagtc
      301 actccttaaa gcagcaaata ccgggcctca tgcaacacgt aattattgtc tgattttgct
      361 ttgttttatt catggtttcc gggcgagtga aatttgtcga ttgaggattt cggatattga
      421 tcttaaagca aagtgtatat atatccatcg attaaaaaaa ggcttttcaa caacgcaccc
      481 gctattgaac aaagaagttc aggctttaaa aaactggttg agtatccgta cttcttatcc
      541 gcatgctgag agcgagtggg tatttttatc acgtaagggg aatccgcttt ctcggcaaca
      601 gttttaccat attatctcga cttctggtgg taatgccggg ttgtcactgg agattcatcc
      661 gcacatgtta cgctattcgt gtggttttgc tttggcgaat atgggaatag atacgcgact
      721 tatctaggat tatcttgggc atcgcaatat tcgtcatact gtctggtata ccgccagcaa
      781 tgcagggcgt ttttacggca tctgggatag agccagagga cgacagcgtc atgctgtttt
      841 atagagatag ttgagatacc agggatggtg ttaaaaacta cattattctt acgatatatc
      901 aaaaatgatt aaccatttat taattgatgt tattgtaagt gtgattctta attatcataa
      961 ta
//
LOCUS       DAZPCS010000398          957 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZPCS010000398 DAZPCS010000000
VERSION     DAZPCS010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..957
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.406"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            1..241
                     /locus_tag="ACHUQ7_004328"
                     /pseudo
     CDS             1..241
                     /locus_tag="ACHUQ7_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            258..776
                     /locus_tag="ACHUQ7_004329"
                     /pseudo
     CDS             258..776
                     /locus_tag="ACHUQ7_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
BASE COUNT          248 a          252 c          233 g          224 t
ORIGIN      
        1 ggtgtatatc cgcactcgcg aaacatatgg aacccggcgg ctccagacgg agctggcaga
       61 taacggaatt atcgtcggtc gtgacagact ggcaggtctt cgtaaggagc tgaggctgca
      121 ctgtaagcag aaacgcaagt tcagggcgac cacaaactct gaccacaatc tgccggttac
      181 gccgaatctg ctgaatcaga actttacgcc cacagcacct aatcaggtct gggtcgcaga
      241 ttcagttgtc caggccttcc gaaatcaacc gactgaaggc gctggtcgcg aaactgcagc
      301 gtatgcagtt cggtaaaagc tcagaaaaac ttcgcgcaaa aaccgaacgg tggatacagg
      361 aagcacagga gcgaatcagc gcacttcagg aggaaatggc ggaaacactg ggtgagcaat
      421 atgacccggt actgccatct tccctgcgcc agtcttcagc ccgtaaaccg ttaccagcct
      481 cacttccccg tgccccccgg gtcatccggc cggaagagga atgctgtccg gcctgtggtg
      541 gtgaactcag tccactggga tgtgatgtgt cagagcagct ggagcttatc agcagcgcct
      601 ttaaggttat cgaaaaacaa cgccctaaac tggcctgtcg ccggtgcgac catatcgtgc
      661 aggcaccggt gccttcaaaa cccattgcac gcagttatgc cggagcgggg cttctggccc
      721 atgttgttac cggaaagtat gcagaccatc tgccattata ccgccaaagt gatctactat
      781 tccacacagc tatttagctc tctttttgtt gagctcccat gattctttca tttattgtta
      841 ctaattattt tatgttatca tgatgttaga acacgctctg ctctgtgtct ctattttatc
      901 tatcatattc tgaggattaa ctacataaca aggcgcaaga tatatataca aaaacaa
//
LOCUS       DAZPCS010000399          952 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZPCS010000399 DAZPCS010000000
VERSION     DAZPCS010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..952
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.407"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            104..>952
                     /locus_tag="ACHUQ7_004330"
     CDS             104..>952
                     /locus_tag="ACHUQ7_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR1137412.1"
                     /translation="MIKSTNIQVIGSGIMHQINNIHSLTLFSLPVSLSPSCNEYYLKV
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQGAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTYLPDFPPMLKVIHAQFNQLESLPALPETLEELNVGDNKIKELPYLPETLTHLRIHN
                     NRLHILPLLPPELKLLIVSGNRLDSIPPFPDKLEGLALANNFIEQLPELPFSMNRAVL
                     MNNNLTTLPESVLRLAQNAFVNVAGNPLSGHTMRTLQQITTGPDYSGPRIFFSMGNSA
                     TISAPEH"
BASE COUNT          322 a          193 c          163 g          274 t
ORIGIN      
        1 aagcaatacc tggagaaaga gtacaaagca aactgtatta aacttaataa gttatattgt
       61 atttcactag agttcttatc gttgataaga aattctggtt ataatgatta aatcaacgaa
      121 tatacaggta atcggttctg gtattatgca tcaaataaac aacatacact cgttaactct
      181 attttctttg cctgtgagtt tgagtccatc ttgtaatgaa tattatttaa aagtctggag
      241 tgaatgggaa aagaacggta ccccaggcga gcaacgcaat atcgccttca ataggctaaa
      301 aatatgtttg caaaatcaag gggcggaatt aaatctatct gagttagatt taaaaacatt
      361 acctgattta ccgcctcaga taacaacact ggaaataaga aaaaacctat taacatatct
      421 ccctgatttc ccaccaatgc ttaaggtaat acatgctcaa tttaatcaac tggaaagctt
      481 acctgcctta cccgagacgt tagaagagct taatgtgggt gataacaaga taaaagaatt
      541 accatatctt cctgaaactc taactcattt acggatccat aataaccgat tgcatattct
      601 gccactattg ccaccggagc taaaattact gatagtttct ggaaacagat tagacagcat
      661 cccccccttt ccagataagc ttgaagggct ggctctggct aataatttta tagaacaact
      721 accggaatta ccttttagta tgaacagggc tgtgctaatg aataataatc tgacaacact
      781 tccggaaagt gtcctgagat tagctcagaa tgccttcgta aatgttgcag gtaatccatt
      841 gtctggccat accatgcgta cactacaaca aataaccacc ggaccagatt attctggtcc
      901 tcgaatattt ttctctatgg gaaattctgc cacaatttcc gctccagaac ac
//
LOCUS       DAZPCS010000400          951 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZPCS010000400 DAZPCS010000000
VERSION     DAZPCS010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..951
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.408"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(304..441)
                     /locus_tag="ACHUQ7_004331"
                     /pseudo
     CDS             complement(304..441)
                     /locus_tag="ACHUQ7_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(523..821)
                     /locus_tag="ACHUQ7_004332"
                     /pseudo
     CDS             complement(523..821)
                     /locus_tag="ACHUQ7_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          270 a          215 c          174 g          292 t
ORIGIN      
        1 tgctaatcta aaaaaaccat tgccacattg aatggctata ttcaccaata aattctttcg
       61 atttcgtata ttgacatatc gtagatatat cttatcccta agattcaata atcgagtccg
      121 tttaccattc tgtgcaagcg ccttcactca gaagtgaccg tcaaggcgat caccaaactt
      181 gataataaag cagctcattg ccatacgcca gtccttaagg ggcgttgtcc atttctgtga
      241 tgctgcctag atttaactgc cagctccggc ttatctacat ctaaatatgt gagcctaata
      301 ccgctacact ttgtccagac tatgtttttc tccgggaagt gggctaaccg tctccataaa
      361 gtgacgaact tttttcaaca gtggccatat tgaacggcac tgatgattac gggttattgt
      421 ctcatgaaga gccggccaca tcactgacag cagtgacttc aggccttcag caccagacag
      481 cctgaaccag ttaatccatc gcccgacaaa tttagtaatc tagggctggt ttttcttcag
      541 ctgttgcggt tcgaaagtgc cattacgatc acgcagagta cggatttcca gaggttcatc
      601 tccagtagtg ataatcttcg ttaagcaacc attgcgggaa ttgctgccag atctaagctg
      661 atttttctcg cacccaagat gatgagtcat attagcattg agtacggttt cattttcagc
      721 atccaatcaa actgactgag atcttcagga tttttgagac ttttggctag ttcattagct
      781 aaagcccgca acttattaga tgtcaactgt ttttcattca taaattcacc ttcatttgat
      841 atcaaattaa atagttcaac atcagagaat tatacaaact tatgtacagg ctcctatgat
      901 cttcgaatcc tgaagattat agtgaaaata agttttcacg ttgaaatccc a
//
LOCUS       DAZPCS010000401          939 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZPCS010000401 DAZPCS010000000
VERSION     DAZPCS010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..939
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.409"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            173..>939
                     /gene="ipaH7.8"
                     /locus_tag="ACHUQ7_004333"
     CDS             173..>939
                     /gene="ipaH7.8"
                     /locus_tag="ACHUQ7_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HFR1137413.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKEL
                     NADNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLK
                     FLHVYYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPE
                     SFSQLDSSYRINISGNPLSTRVLQSLQRLTSSPDYHGP"
BASE COUNT          320 a          193 c          131 g          295 t
ORIGIN      
        1 ccacaataaa aaaagaattc catagaaaac ctccataaat aaattacaac taacttctgt
       61 tatgtgtaaa atggaaacta ttaaaactta atatcggaaa tggtaagtga aatttgtata
      121 aatatacaat tttaaatatt tattctcaca aatataaggt tgacctagca ttatgttctc
      181 tgtaaataat acacactcat cagtttcttg ctccccctct attaactcaa actcaaccag
      241 taatgaacat tatctgagaa tcctgactga atgggaaaag aactcttctc ccggggaaga
      301 gcgaggcatt gcttttaaca gactctccca gtgctttcag aatcaagaag cagtattaaa
      361 tttatcagac ctaaatttga cgtctcttcc cgaattacca aagcatattt ctgctttgat
      421 tgtagaaaat aataaattaa catcattgcc aaagctgcct gcatttctta aagaacttaa
      481 tgctgataat aacaggcttt ctgtgatacc agaacttcct gagtcattaa caactttaag
      541 tgttcgttct aatcaactgg aaaaccttcc tgttttgcca aaccatttaa catcattatt
      601 tgttgaaaat aacaggctat ataacttacc ggctcttccc gaaaaattga aatttttaca
      661 tgtttattat aacaggctga caacattacc cgacttaccg gataaactgg aaattctctg
      721 tgctcagcgc aataatctgg ttacttttcc tcaattttct gatagaaaca atatcagaca
      781 aaaggaatat tattttcatt ttaatcagat aaccactctt ccggagagtt tttcacaatt
      841 agattcaagt tacaggatta atatttcagg gaatccattg tcgactcgcg ttctgcaatc
      901 cctgcaaaga ttaacctctt cgccggacta ccacggccc
//
LOCUS       DAZPCS010000402          938 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZPCS010000402 DAZPCS010000000
VERSION     DAZPCS010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..938
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.410"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..132
                     /locus_tag="ACHUQ7_004334"
     CDS             <1..132
                     /locus_tag="ACHUQ7_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HFR1137414.1"
                     /translation="LKVDPLKCELRGGQMRFTGLKRGYRLAEQVMMHEPLARIRWCG"
     gene            319..556
                     /locus_tag="ACHUQ7_004335"
                     /pseudo
     CDS             319..556
                     /locus_tag="ACHUQ7_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            670..928
                     /locus_tag="ACHUQ7_004336"
                     /pseudo
     CDS             670..928
                     /locus_tag="ACHUQ7_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
BASE COUNT          292 a          176 c          255 g          215 t
ORIGIN      
        1 ctgaaggttg acccgctgaa gtgcgagctg cgcggaggtc agatgcggtt tacggggctg
       61 aagaggggtt accgtctggc agagcaggtt atgatgcatg agccgctggc tcgaatacgg
      121 tggtgcggct gagagtcgca gaggggaagt tgcgtccatg ttgaggggaa cggagcaaaa
      181 aaccatcaga gatacattga atcaatcaat gtaagcaata ttaagtgtct ataccgatta
      241 cttgatgatc aggttgtgtt gaataaacgc gcattcttcg gtgctttttt aatcttttgg
      301 ggatttaaat tcctatcgat gaactgtcga tatgaaaaag cgagcatcat actgacgtca
      361 aacaaagggg tcgcagactg gggagaaatg ttcggagatc acgtgctggc aacagcgatc
      421 ctagatcggc tgctacacgc tgaatatcaa aggagagagt taccggttaa aagagaaacg
      481 taaagctgga gtgctgacca aaaacacaac gccaatcagt gatgatgaaa tggtggaaaa
      541 cggacagcat cagtaacgaa agtatcttag caggcatgaa aatcgtccgc atcccctaaa
      601 cggtcaaaat gcttctgccg tgaaggaagg agaatgccat ctcatcactt atatctggcg
      661 aatgaaacac agatggtcgg ccttgccccc aaacacacgg cggtgcaaaa tggaatggca
      721 gaaaacttcg tcaaaacgat gaaatatgat taaatcagtg ttatgttcga acctgacgtg
      781 ttaacagcag taaagtagtc tcgcgaaggt gttcaaacat tataatgaat ggcatcctca
      841 tagtgcgcag ggttatcgct tgctgcggaa atatctgaag cgacgtacca gtaatgggga
      901 aaatgatagc tggtttctga aaatatagag gaaaatgt
//
LOCUS       DAZPCS010000403          936 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZPCS010000403 DAZPCS010000000
VERSION     DAZPCS010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..936
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.411"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            144..641
                     /locus_tag="ACHUQ7_004337"
                     /pseudo
     CDS             144..641
                     /locus_tag="ACHUQ7_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            682..873
                     /locus_tag="ACHUQ7_004338"
                     /pseudo
     CDS             682..873
                     /locus_tag="ACHUQ7_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          244 a          232 c          269 g          191 t
ORIGIN      
        1 cttattatgc ttccatcgga aacgatgatc tggcagcctg agttcacaga taaaacactc
       61 tccaggaaac ccggggcggt tcaaagcgcc agccccgtcc cgcccgatag agcgcggcat
      121 agcgggcccc ggattacttg cgtgcgacgg cagaagaagc cctgaagcga atcagtgaac
      181 tgtacgccat cgaggatgaa atacggggat taccagagtc agagtgtctt gcagtcaggc
      241 agcagcgaag caaagcgtta ctgacgtcgc tgcatgaatg gatggtggag aagaatggca
      301 cgctgtcgaa aaaatccaga ctgggcgaag cattcagcta tgtactgaat cagtgggacg
      361 ccctctgtta ttacagtgat gacggtctgg cggaggcgga caataacaca gcggaaagag
      421 cgcttcgtgc agtctgtctc ggaaagaaaa attacgtgtt cttcggtagc gatcacggcg
      481 gcgagcgtgg tgcactgctg tacgggctga tcggcacctg ccgtctgaac ggtatcgatc
      541 cggaagcgta tctgcgccat attctgagcg tactgccgga atggccttcc aaccgagttg
      601 acgacctcct gccatggaag gtggtgctac catcaggata atcgtcaata cggtgttcgg
      661 tgaacgttta cttggagcat catgcttcat gaagaaaaac tggcacgtca tcaacgtaaa
      721 caggcgatgt atacccgaat ggcagccttc ccggcggtga aaatgttcga agagtatgac
      781 ttcacgttcg ccaccggagc accgcagaag cagctccagt cgttacgctc actcagcttc
      841 atagagcgca atgaaaacat agtcttactg gggacatctg acatcacaaa tccccgggtg
      901 gggatttgtg tataagagac agcccatgga ggaagg
//
LOCUS       DAZPCS010000404          935 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZPCS010000404 DAZPCS010000000
VERSION     DAZPCS010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..935
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.412"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(282..764)
                     /gene="sseA"
                     /locus_tag="ACHUQ7_004339"
                     /pseudo
     CDS             complement(282..764)
                     /gene="sseA"
                     /locus_tag="ACHUQ7_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
BASE COUNT          222 a          248 c          221 g          244 t
ORIGIN      
        1 taattttgag caggatttgt ttatatttgt ctgaattcgg actaataagc caggttattt
       61 tattaccgaa cacggcggaa agggccggtg tcgataatac gctaaataaa caataattac
      121 tctcttttgc ttgacaaaaa gagagttact ggtgagtatt gttttgctgt gaagcacctg
      181 cgttgctcat gtaacacaag tcaacctgct ggaaaggccg cgaaccagac cagcaataaa
      241 aaaacgccaa attcggcggt tttttattgc ctgtaatata cttatttcac tggctcaacc
      301 ggtaaatctg cccgcgcgcc ccattcactc catgcgccgt cgtacagttt cacgtttggc
      361 acatccagcg tcgcgagtgc taacaaaacc acggctgccg ttacaccaga gccgcagctg
      421 acgataatcg gtttgtcata gctgacgccg cgaccaaaaa atatcgcatc cagttcatcg
      481 gtcgttttta gttcgccttc gcgcaccagt tccgtccacg gaacgttcaa tgcaccggga
      541 atatgtccgc gacgtaaacc tgggcgaggt tcatcaactt ctgcgttaaa acgtgcagcc
      601 gggcgggcat caataatttg cgccgtattt tcatggcttg ccaataacac atcggttact
      661 ttcaccactg cttcaggatt aaacgcggcg ttaaactctc cttccggcag ctctactgca
      721 ccttcttcta atagcaggtc atcgcgctgc cagcctgcaa gtccccgtta ctttattcgt
      781 accccttata atggggtgtt agccagccag acccggcatg attactgccc ccagtcgtcc
      841 atgatccggg gggggatgtc accgggtctg gtggggcgct ggtaaccgct aataggggtc
      901 aggtcaggca cttttgccgg gaccgtctgt aacgt
//
LOCUS       DAZPCS010000405          935 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZPCS010000405 DAZPCS010000000
VERSION     DAZPCS010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..935
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.413"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(57..245)
                     /locus_tag="ACHUQ7_004340"
     CDS             complement(57..245)
                     /locus_tag="ACHUQ7_004340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1137415.1"
                     /translation="MTNEELQAIALSMLERQREKVHQQRESLLNPSPFTRPKRLELEA
                     FLDKYPRRLKSGKTRPPG"
     gene            complement(542..780)
                     /locus_tag="ACHUQ7_004341"
                     /pseudo
     CDS             complement(542..780)
                     /locus_tag="ACHUQ7_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088287.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          246 a          181 c          217 g          291 t
ORIGIN      
        1 taccgcgctg atggttctta tccgttttat tgaatcggag aacaaactgt gagctatcag
       61 cctggtggtc tggttttgcc tgatttaagc cgccgtgggt atttgtccaa gaatgcttca
      121 agctccaatc gttttggcct ggtgaatgga gatgggttaa gcaaagactc tcgttgctgg
      181 tgtacttttt cgcgctggcg ctcaagcatg gataatgcga ttgcctgtag ttcttcgtta
      241 gtcattgtct gcacctggaa agcaatttac cggagtacct cacactacgg caaaaatgtt
      301 ttattgttgc aacttgcgta attattcagg tcagtttgga acgcactgta taaacataaa
      361 taacctgata ctgttgtgtg ttttcaaaga aaacacaggt atttttcaca taaaccttcc
      421 ccttttcata ttcgaccact taatcaaaaa acggttttat acttgttgcc cccactgggg
      481 ttaaatgtga gacatgtcgc actttgtgcc atgtgtcctc gctgatttta ggcgcggttc
      541 attcattctt tctgttggca taaagtatgg tatcggttgg gcgaatgaaa gggactcggg
      601 tcatggtcag acaatacctg aggcgttaat tcgttgaaag agattgtgaa tgggggaata
      661 actgagtctt aactgctgtg cgatagcccg gacagatagt tgctcgtcga atctgagacg
      721 taatgattct ggcaaataag tgcgaaagcc ggtgatatct agtgttgttt gagagttcat
      781 cgttcgcgtc cattaataca gatggacgcg attgttgcca gacaggataa ttttcacaag
      841 acatcgcaga tagagtactt acgataattt tttattttat acaacagatg cccacgaaaa
      901 gaattatttt caatagacac ctgcattaca caaag
//
LOCUS       DAZPCS010000406          925 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZPCS010000406 DAZPCS010000000
VERSION     DAZPCS010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..925
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.414"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            119..>925
                     /gene="virB"
                     /locus_tag="ACHUQ7_004342"
     CDS             119..>925
                     /gene="virB"
                     /locus_tag="ACHUQ7_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HFR1137416.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIK"
BASE COUNT          362 a          165 c          149 g          249 t
ORIGIN      
        1 tattaaaata gtaattttta agactaccgt tgactatcat caacagttac atacaatctt
       61 gctcacactg catttaactt ttgtcaatat aaatcaatat gaataaacag ggtgtgatat
      121 ggtggatttg tgcaacgact tgttaagtat aaaggaaggc caaaagaaag agtttacact
      181 ccattctggt aataaagttt cctttatcaa agccaagatt cctcataaaa ggatccaaga
      241 tttaaccttc gtcaaccaaa aaacgaatgt acgcgatcaa gaatccctaa cagaagaatc
      301 attagccgat atcataaaaa ctataaagct acaacaattc ttccctgtaa taggaaggga
      361 gattgatggt agaattgaaa ttctggatgg cactcgtaga agagcatctg caatatatgc
      421 aggagcagat cttgaagttc tatattcaaa agaatatata tctactcttg atgccagaaa
      481 actagcaaac gatatacaaa cagcaaaaga gcatagcatc cgagaacttg gtattggtct
      541 taattttctg aaagtatcag ggatgtccta taaagacata gccaaaaaag agaatctgtc
      601 tcgcgcgaaa gtcactcgtg cctttcaggc agcaagcgtt ccacaggaaa taatatctct
      661 atttccaatc gcgtcagaac ttaactttaa tgactacaag atattattca attattataa
      721 aggacttgaa aaggctaatg aatctcttag ttctacacta ccaatattaa aggaagaaat
      781 aaaagacctt gatacaaatt tgcccccgga catatataaa aaagaaattt taaacatcat
      841 aaagaaaagc aaaaacagaa agcaaaaccc ttcgctaaaa gttgactcgt tatttatttc
      901 taaagacaaa cggacttaca taaaa
//
LOCUS       DAZPCS010000407          923 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZPCS010000407 DAZPCS010000000
VERSION     DAZPCS010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..923
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.415"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            1..200
                     /locus_tag="ACHUQ7_004343"
                     /pseudo
     CDS             1..200
                     /locus_tag="ACHUQ7_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415867.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            300..>923
                     /locus_tag="ACHUQ7_004344"
     CDS             300..>923
                     /locus_tag="ACHUQ7_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
                     /protein_id="HFR1137417.1"
                     /translation="MSTKPLFLLRKAKKSSGEPDVVLWASDDFESTSTTLDYLLVKSG
                     KKLSNYFKAVATNFPVVNDLPPEGEIDFTWSERYQLSKDSMTWELKPGAAPDDVHHQD
                     NAQETKELAGGQEENAQADAHEDCQDCEVSVATLRFTQRLLHIFTYAAGDLKYLHHAT
                     REQRKHITALEMDQENSYVQNLLLAIRGMAEPTTLDNAALLRLTDAIK"
BASE COUNT          293 a          217 c          205 g          208 t
ORIGIN      
        1 attggctacg taattaaaac agaaaataaa gaaaccccat tcactgtagt tgatatcgac
       61 ggtctatcag gaaactttaa aactcttgtt gaaggtgcca caaaaatgtg cctagtttac
      121 atcggaaata atctgcccgc agaaaaaaag ccgaatttct ggcaaatctg attgcaatga
      181 aattaaaagg tgaaatctga aaaaaagaaa gcctgcacag tgtgcaggcc tgagtgaaga
      241 acctgggaca tttattcatc actcgcagta attttaatct gagttgaggt taaaaaacaa
      301 tgagcacaaa accactcttc ctgttacgga aagcgaaaaa atcatccggt gaacctgacg
      361 tcgtcctgtg ggcaagcgac gattttgaat cgaccagcac cactctggac tatctgctcg
      421 ttaagtcagg taaaaaactg agcaactatt ttaaagctgt tgccacaaat tttcctgtcg
      481 ttaatgacct tccccctgaa ggtgagatcg attttacctg gagtgaacgc tatcaactca
      541 gcaaagactc catgacatgg gaactaaaac cgggagcagc gccagacgac gttcaccacc
      601 aggataatgc tcaagaaacc aaagaactgg cgggaggcca ggaagaaaac gcgcaggcag
      661 acgcccacga ggattgccag gattgcgaag tctctgtagc cactttgcgg ttcacacagc
      721 gtcttctgca catttttacg tatgctgccg gggatctgaa atacctgcat catgccaccc
      781 gtgaacaacg caaacacatt actgctcttg agatggatca ggaaaacagc tatgtccaga
      841 atctgctgtt ggccatacgc ggcatggcag aaccgacaac tctggataat gccgccctgc
      901 tccgcctgac tgatgcaatt aag
//
LOCUS       DAZPCS010000408          894 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZPCS010000408 DAZPCS010000000
VERSION     DAZPCS010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..894
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.416"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
BASE COUNT          281 a          177 c          163 g          273 t
ORIGIN      
        1 gtaacaagta aaatcaacat aaaaaattca gcaaaattta atatttgtta aactagccgt
       61 ctcaaatatg acattattag ttaactgcat ttgccacaaa atggctattt catggaacgc
      121 agtaatttct tagtggctat tatcatgcta attcttagat ccctagcgat tattcctgcc
      181 tggttcgtca gcttaggcca tgagattgtt acattccttt ttatcctgga gatatggggg
      241 aacgcaggcg acgctttttc atctacatcc gttggtaaat atagctttgg tcactacgac
      301 ttaagccttc ctcaatcaca gctattcgcc gggcgatacg gcaaacaaac taagtgaaac
      361 atcatcctga aaacaccaac atcaacaagc ctctccagat cgacatcagg agtgaccagt
      421 tacaagccac aaaccaagaa acatatttca tgatgagaat tatgctcagc ctgatggcga
      481 gtaaaccctt attttcacca ctctcactga gttagcttgc aaagctttct ttgtttagat
      541 tctaaataaa ttatttataa aacgtatagt taaaagaaaa tctttagttt ttgaagacca
      601 ccgcaagtgt tcgtctggct tcacatggca tcttcttctt agaaaaagat cgacatattt
      661 tgtgacgcga attgcaaatc tggttttgtt gtatggattg cgtgattttt gatctggtat
      721 aacaggtata aagatgcacc acgatagtca atgagactgg gcatctcgta atctatggca
      781 aacatcactt cagttctttc acatcgggtg atgaaaatgc acttcagtct gaaaggaata
      841 tgaaaatgag atcaacagac actctatttt atgactctgg gtaaaatggg ttga
//
LOCUS       DAZPCS010000409          887 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZPCS010000409 DAZPCS010000000
VERSION     DAZPCS010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..887
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.417"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            270..743
                     /locus_tag="ACHUQ7_004345"
     CDS             270..743
                     /locus_tag="ACHUQ7_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1137418.1"
                     /translation="MSWLISQCAHQCTDNKKTETDAIYDKVRSSYLLSCILKKNKNVG
                     LILHAPSFVSVSEKIARIVMANYSRNWSNSELASAVLMSESSLKRRMYKEVGSISTFV
                     HKIKLTEAIRKLRRTNTPISVISSELGYSSPSYFSKVFFKYLKTYPQNIRKKNGR"
BASE COUNT          270 a          186 c          197 g          234 t
ORIGIN      
        1 aatcgctgga ttcggtcatt ctgtctccct tgtatatggg ttaaccagat aaccacgggg
       61 agcaggtacc ggcatccacg ttacagacgg tcccggcaaa agtgcctgac ctgaccccta
      121 ttagcggtta ccagcgcccc accagacccg gtgacatcac cccccggatc atggacgact
      181 gggggcagta atcatgccgg gtctggctgg ctaacacccc attataaggg gtacgaataa
      241 agtaacgggg gggagatgaa ccctatgcaa tgtcctggtt aatatctcag tgtgctcatc
      301 agtgcactga taataaaaag acggagacag atgccattta cgataaagtt cggagttctt
      361 atttgctttc atgtattctg aaaaaaaaca aaaatgttgg gcttattctt catgcaccat
      421 catttgtttc tgtgtctgaa aaaatcgcca gaattgtgat ggcaaattac tcccgtaact
      481 ggagtaattc cgagttggcc agtgcggttt taatgagtga atcttcttta aaaagaagaa
      541 tgtacaaaga ggtcggttct ataagtacat ttgtacataa aataaagctt acagaagcta
      601 tcaggaaatt acgaaggaca aatactccca tttctgtcat atcatctgag ttaggttaca
      661 gttcaccatc gtatttttct aaagtcttct ttaaatattt aaagacatac cctcaaaaca
      721 taaggaagaa aaatggtcgg taatgtcacc aattaggagg acttgcagag ccgctttttt
      781 acgatactga gatcaaacag acataatcta tgcggagatt tacagcaggg catggttcac
      841 aaatctgaca gcgatgaatt atcggcactg cgggcagaaa atgctcg
//
LOCUS       DAZPCS010000410          875 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZPCS010000410 DAZPCS010000000
VERSION     DAZPCS010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..875
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.418"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(22..693)
                     /gene="queE"
                     /locus_tag="ACHUQ7_004346"
     CDS             complement(22..693)
                     /gene="queE"
                     /locus_tag="ACHUQ7_004346"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFR1137419.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          237 a          215 c          189 g          234 t
ORIGIN      
        1 ataacgctta taaatgtgta atcaggcaat atttaaatat ttatgtgttt gcatcgacaa
       61 acgccaatta cgcgcaatac aggtttcaat gcacaatcgt gtggcatcat ccttttggct
      121 aatcggctgc agtgcaatga ctcgcggttt atcatcggtc agcgttgcca gtagttcatc
      181 cagtgcttca atatcgcgta cgcgccccac tggatgcttg atttcgttgg ctcgctccag
      241 tgcctgtgac aacacttcat agccgccgcg catgttcagc tttggcgata cggtaaccca
      301 ggtattcggt gtgcagcgta cttcatgagt tccactggtt tcgatctggc agctaaaacc
      361 gttcttttcg agcaggtcag tcagtggcag caaatcatga atgcaaggct caccacccgt
      421 aatcaccaca tgccgcgcgg tatatccctg acgaccaatg acagccagca agtcttcact
      481 gctcgccgcc ccccacttat cactctcttt ggtcttcgcc agaatgctga aaagggagac
      541 ttcccgatcc tcaagctttt cccaggtgtg tttggtgtcg caccaggcac agccaaccgg
      601 gcatccctgt aaacgaataa aaatggcggg aacgccggta aagtaaccct caccttgcag
      661 ggtctggaac atctcgttaa tcgggtactg catagcattc tctgtgaagt ggataattgt
      721 taattattgc agatcctgcc gcaacaatca tgtcttatta acattctgtt acatgcagat
      781 ttaaagaaaa atacgaaaca tatcgttaat aattaaaggg agtaacgtat tatgtcagaa
      841 gaaaataaag aaaatggatt taatcatgtc aaaac
//
LOCUS       DAZPCS010000411          873 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZPCS010000411 DAZPCS010000000
VERSION     DAZPCS010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..873
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.419"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..>873)
                     /gene="ospC4"
                     /locus_tag="ACHUQ7_004347"
     CDS             complement(<1..>873)
                     /gene="ospC4"
                     /locus_tag="ACHUQ7_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
                     /protein_id="HFR1137420.1"
                     /translation="NPNKATKALQKNVLRVTNSSSSGISEKHLDHCANTVKNFLRKSI
                     AAQSYSKMFSQGTSFKSLNLSLEAPSGARSSFRSLEHLDKVSRHYISEIIQKVHPLSS
                     DERHLLSIIINSNFNFRHQSNSNLSNNILNIKSFDKIQSENIQTHKNTYSEDIKEISN
                     HDFVFFGVEISNHQEKLPLNKTHHTVDFGANAYIIDHDSPYGYMTLTDHFDNAIPPVF
                     YHEHQSFFLDNFKEVVDEVSRYVHGNQGKTDVPIFNTKDMRLGIGLHLIDFIRKSKDQ
                     GFREFCYNKNIDPVS"
BASE COUNT          281 a          135 c          152 g          305 t
ORIGIN      
        1 actaacagga tcaatatttt tgttataaca aaactctctg aatccttgat ctttactctt
       61 tcttataaaa tcaataagat gcaacccaat tccaagcctc atatctttag tgttaaatat
      121 tggaacatca gttttcccct gattaccatg cacatacctg ctaacttcat ccacaacctc
      181 tttaaagtta tctaagaaaa atgattggtg ctcatggtaa aaaacaggtg gaatagcatt
      241 atcaaagtga tcggttaatg tcatatatcc atatggagag tcatgatcaa tgatatacgc
      301 attggcgcca aaatctacag tatgatgtgt tttatttagt gggagttttt cttgatgatt
      361 ggaaatttcg accccaaaaa aaacaaaatc gtggttagag atttctttaa tatcctcaga
      421 gtatgtattc ttatgtgttt gtatattttc agactgaatt ttatcaaagg atttaatatt
      481 aagaatatta tttgacagat tagagttact ttgatgtctg aagttaaaat tagaattaat
      541 aataatagaa agtaagtgac gttcgtctga agaaagagga tgaacttttt gtattatctc
      601 agagatatag tgcctggaca ccttatcgag atgctcaagt gaccgaaatg atgaccgtgc
      661 tccagatggc gcttcaagag aaagatttag tgatttgaaa ctggtccctt gagaaaacat
      721 tttgctataa ctttgcgctg ctatagactt ccttaaaaag tttttcacag tattagcaca
      781 gtgatctaaa tgtttttcac ttatgccaga agaagatgag tttgttacgc gtaatacatt
      841 tttttgtaat gcttttgttg ctttattcgg att
//
LOCUS       DAZPCS010000412          871 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZPCS010000412 DAZPCS010000000
VERSION     DAZPCS010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..871
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.420"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..323
                     /locus_tag="ACHUQ7_004348"
     CDS             <1..323
                     /locus_tag="ACHUQ7_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFR1137421.1"
                     /translation="NICNKKHKQHMDDHLLHLIRSNAIAPTPVYENYFNNTTAFRPKP
                     EDIFKCGQTLKKTKQKFGHYGLGVDQHQKIDNYTMGLNSWKIAPNERGDKKGVPGLSC
                     FQPQ"
     gene            585..>871
                     /locus_tag="ACHUQ7_004349"
     CDS             585..>871
                     /locus_tag="ACHUQ7_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1137422.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMR"
BASE COUNT          311 a          140 c          163 g          257 t
ORIGIN      
        1 caaatatatg taataaaaaa cataaacaac acatggatga tcatcttctc catctaatta
       61 gaagcaatgc tatagcaccc acacctgtat atgaaaatta ttttaataac actactgctt
      121 tcagaccaaa acctgaagat atcttcaaat gtggacagac gctcaaaaaa actaaacaga
      181 aatttggcca ttatggatta ggggtagacc agcatcaaaa aattgataat tataccatgg
      241 ggctaaattc ttggaagata gcacctaacg aacgtggtga taaaaaaggt gtcccagggc
      301 ttagttgttt tcaacctcaa taaaacttca tgagtgctgt ggtatctctt tttaattttg
      361 ccagtatttc ataggttttc ttaatcgatt gtagaggtat gtactgatga cgcctctaca
      421 atatttaggg actttctctg ctatgagata agaaatttca tattatgctt taaggctgaa
      481 tgatttgtgc aaaatataat tatttcccca tcgaatatga ggtgtatgtc ataagataaa
      541 atgttaacta tgtattcctc gcggtgaata atatgaggta aaagatgaaa attggaactg
      601 tggcaggcac taatggcagt acaacgacaa tcgcaacaaa tgatatggtg caagagcatg
      661 ttaccaactt cactaaagaa ctgtttgggt atattgcaaa tggtataggt gatgatatat
      721 caagtattgc caggacaatg ctcggtgaag ttgtggaaaa aattgatgat tggcaaatag
      781 aacgtttcca acaatctatt caagatgaca aaatttcatt taccatacaa acgaatcatt
      841 cagaaaaata tagtatgcta tcgggcatgc g
//
LOCUS       DAZPCS010000413          870 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZPCS010000413 DAZPCS010000000
VERSION     DAZPCS010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..870
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.421"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..>870
                     /locus_tag="ACHUQ7_004350"
     CDS             <1..>870
                     /locus_tag="ACHUQ7_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001689732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFR1137423.1"
                     /translation="LSSAAVKTAGVLISFQAVLSAYQRVMEVGLQRASAERSIDFVFS
                     KDANQVKDFTKSLAQTTGLDIAELQSQFAGFGASARESLGIQGSEELFRNMIGYSRLM
                     GRSEEEIKRALTALSQMAGKGQIMAEELKGQLAEAVPGMVQVFAKATGKTEQELFDAM
                     KKGALKSADTLQKVTQELNKQITAKGGWKAISESTQAQLGNLKNSWNTTLDSIFRGSE
                     NGLQDFTRSLTNLLNALGGSGKSLGESLGSLMTSMSHGVDSLTDISYRVRAFFDEVTL
                     AYRPLNDTQRKIV"
BASE COUNT          263 a          153 c          209 g          245 t
ORIGIN      
        1 tttaagttca gcagcagtta aaacagcagg tgtacttatc agctttcagg ctgtactatc
       61 cgcttatcag cgagtaatgg aagttggcct tcaacgtgca agtgctgaac gttcaattga
      121 tttcgtattt tctaaagatg caaatcaagt aaaagatttc acaaagtcat tagctcaaac
      181 aacgggttta gatatcgctg aactgcaatc acagtttgct ggtttcggtg cttcagctag
      241 agagtctcta ggcattcaag gttctgaaga actttttaga aatatgattg gctattctcg
      301 tctgatgggg cgaagtgaag aagagattaa gcgagcactg acagcattat cacagatggc
      361 tggtaagggt cagattatgg ctgaagagct taaggggcaa ctagctgaag ctgttcctgg
      421 tatggttcag gtattcgcta aagcaaccgg aaagactgaa caggaattat ttgatgctat
      481 gaagaagggg gctttaaaat ctgccgatac tcttcagaag gtcactcagg aactgaataa
      541 acaaatcacc gctaaaggtg gctggaaagc tatttcagaa agtacacagg cacaactagg
      601 gaacttgaaa aactcttgga ataccacgct tgatagtatc ttcagaggtt ctgaaaatgg
      661 tctgcaagat tttacaagaa gcttaaccaa tcttttaaac gctcttggtg gaagtggtaa
      721 gagtcttggt gaatctcttg gtagtctcat gacttctatg tcacatggtg tcgattcatt
      781 gaccgatatt agttacaggg taagggcgtt ctttgatgag gtgacattag cttaccgtcc
      841 actaaatgac actcagagga aaattgttga
//
LOCUS       DAZPCS010000414          859 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZPCS010000414 DAZPCS010000000
VERSION     DAZPCS010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..859
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.422"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            15..859
                     /locus_tag="ACHUQ7_004351"
                     /pseudo
     CDS             15..859
                     /locus_tag="ACHUQ7_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013202912.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
BASE COUNT          262 a          132 c          182 g          283 t
ORIGIN      
        1 atcactcttt taatatcgct gtaattattc cttgttataa tgagcagaag gcaatagcaa
       61 aagtcataaa tgatttcaaa acgaatattc caactgcttc tatatatgta tttgataata
      121 attcgactga cagtacagca caagtggcag aggatgctgg agcaacagtt cactctgttc
      181 ctctgaaagg caaggggaat gtcgtaagac gtatgttctc agatgtagat gcggatattt
      241 atctaatggt tgatggagat gacacttatg atgcaagttc tgctccagaa atgattaacc
      301 atctcataaa aaatcaattg gatatggttg taggatgtcg tcaagagaat ggagatcaaa
      361 atacatacag aaaagggcat cgctgaggaa ataaattgtt gactggtact gttcagacta
      421 tctttaaagg gaattttact gatatgttgt cggggtatcg tgtattttcc agacgctatg
      481 ttaaatcttt tccatgtcta tcaaggggat ttgaaatcga aactgagctt acaattcatg
      541 ctttagaatt acgtatgaag tatggtgagg ttaatacaaa atatggcgag cgatctgaag
      601 ggagtgtgag caaactatca acttggagtg atgggttcaa gattctgaaa acaataatta
      661 aactttactc gcttgaacgg ccactatact tttttagtat tatcggcgtt ttacttgctg
      721 ctttgtctat aattttagga ttgcccatca ttgttgatta tattgataca ggtttggtga
      781 gaagatttcc gactgccttt ttaactgcct ccatcatgtt atcgtcaata atggcatttg
      841 tatgtggcat tattttgca
//
LOCUS       DAZPCS010000415          836 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZPCS010000415 DAZPCS010000000
VERSION     DAZPCS010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..836
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.423"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(52..720)
                     /locus_tag="ACHUQ7_004352"
                     /pseudo
     CDS             complement(52..720)
                     /locus_tag="ACHUQ7_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
     gene            complement(710..>836)
                     /locus_tag="ACHUQ7_004353"
     CDS             complement(710..>836)
                     /locus_tag="ACHUQ7_004353"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase family
                     protein"
                     /protein_id="HFR1137424.1"
                     /translation="MRVRSQGEMDSLVRNAGFDKCTQRIDVWGIFTVSMAVRRDN"
BASE COUNT          271 a          215 c          186 g          164 t
ORIGIN      
        1 ctgcttaaaa aaagaacaaa tggtagtgtc cgctattgcc agtacacctc aatatggcag
       61 tgctattttg attcgacgtt gatcaagttt ctgataattc caacgacggt cgacgggcac
      121 catccagtca atcaacatac ctaccgccag ccctgtgatg acatcaataa aatgatgctg
      181 ccatgtcgtc agcgtcgaaa tggcgatgag taaaaaccat ccgccacaga ctttacgcca
      241 cctcacagcc agatgctgac gaaagtgacg ccaaagtagc cagcagagaa taatatgcag
      301 cgaaggagac tggttataag gcagatcaaa cagttcaagt tgcgaaaata gccatcctgt
      361 caccccactc acttcaggac ggataaaact aaacttcagc gggtagagaa aaaaaccgca
      421 gcaggccatt accgttgcca gaataagccg gtggacaagt cggcgttgtt cgaatgtcga
      481 gctacaaacg aacagcgaaa atccatataa aagatccaga ctccagtaag gaacaatagt
      541 ccagggaaga aaagggatcg ccgtttccca accgaatacc tgactgggga tatcatggct
      601 gttaaggtcc tgaaccgcgg tgaactgatt aagagatcca taggtgaaga agaaaaacgg
      661 ggccagcaac aataaccagc cagcgccttg tagcaatacg ttccgacgtt cagttatcac
      721 gacgcaccgc catcgaaacc gtaaaaatgc cccacacatc aatccgttga gtgcatttat
      781 caaatccggc attacgcacg agcgaatcca tctccccttg cgaacgcacg cgcatt
//
LOCUS       DAZPCS010000416          831 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZPCS010000416 DAZPCS010000000
VERSION     DAZPCS010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.424"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..766)
                     /locus_tag="ACHUQ7_004354"
     CDS             complement(<1..766)
                     /locus_tag="ACHUQ7_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1137425.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSV
                     QTVSSDNKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSKNENRMEALAGCDFIDFNM
                     TGQDMSKLVLTLSKFYFEDLLNINFTDANLFNTIFSH"
BASE COUNT          227 a          124 c          164 g          316 t
ORIGIN      
        1 tgtgtgaaaa aatagtgtta aataaatttg cgtcagtgaa atttatatta agtagatctt
       61 caaaataaaa ttttgataag gttaggacta gcttggacat atcttgccct gtcatgttga
      121 aatcaataaa gtcgcatcca gcaagggctt ccatcctatt ttcattttta ctgtgctcaa
      181 tatacagctt cgccattttt aagtacaagc tttgaatgtc tcttatatca gttggtaagt
      241 cagctcgttg cccagcaaaa tttttattat cggaagaaac ggtttggaca ctgaatcttt
      301 cgggtattcc ttcttctacg agaattttag ttatgattgg attgttgggt tctggattaa
      361 cgacaacaat gatttgacat acagaatcct tatccttttc gtatctaatg ttatgaatct
      421 gattaagtat tgattgtgtc tgttgcgtaa gtggtgtcga acttatcgtt tcaatgttat
      481 taataagact tacaagttga accacaactt ttgagtagtc tgcatttgta tatgtggtgt
      541 tcgtgtggca tgcattaatt tgttggagtt gttgttgcgc taaatcaaat tgttcagggt
      601 tgtatccttc tccagagaat gtggaattaa tgggttgttg ttcttcccaa ttatcacaag
      661 gccacttcgg tgtgtctggc gttttaacat tatcggtatt tgttgtgcag ttataaatat
      721 cgccgtgata tgtatttatt atacctgggg taaagtcaat tgacataaca ttccttattc
      781 atcatctgtt ttatgctaat gtttttaaat tggataatta gaacaggaat g
//
LOCUS       DAZPCS010000417          831 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZPCS010000417 DAZPCS010000000
VERSION     DAZPCS010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.425"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..326
                     /locus_tag="ACHUQ7_004355"
     CDS             <1..326
                     /locus_tag="ACHUQ7_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFR1137426.1"
                     /translation="SETFSVVMTKDPGAACTEQFNILGSFFTTDILSDYSHLDMGNGL
                     LLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANPAETVVEGPFSKDVIL
                     KITYN"
     gene            complement(369..569)
                     /gene="glgS"
                     /locus_tag="ACHUQ7_004356"
     CDS             complement(369..569)
                     /gene="glgS"
                     /locus_tag="ACHUQ7_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFR1137427.1"
                     /translation="MEHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
BASE COUNT          252 a          184 c          154 g          241 t
ORIGIN      
        1 ttagtgagac gtttagtgta gtcatgacaa aagatcctgg tgcggcctgt actgagcaat
       61 tcaatatttt agggagcttt ttcactacgg atattttgag tgattatagc catctggata
      121 tgggtaatgg actgctattg aagatatttc ataaagatgg aacagcaacg gaatttaacc
      181 gcttctcaca atttgcttct ttttcatcgt ctagtgcacc ttcggtcacc gcaccattca
      241 gggcagaact tagtgcgaac ccggcagaga ctgttgttga gggaccgttt agtaaagacg
      301 taatcctcaa aatcacctat aactagcatc taatacaaac actaaaacgg gccatcaggc
      361 ccgttatatc agtgctctaa ctccagctct cttgcctgca tcatctgttg gcaataccag
      421 gcataacgtg cgcaaaacca agttctatgt tcttcatcca tgttaccagt aacagccaga
      481 atatcgaccg ggcgaccttc tgcgtgcatt ctggcaaaac tacgcgccag gaaatcgaaa
      541 ttattcaaag aattaagact atgctccata agcacatcct ccggttatcg catacctttt
      601 aaggccatat gctttttctg atttcagtat tccccaatca gtgtagtgcc tttaaacata
      661 acgtgcgtaa atatattgtc cccgatcaca ctttttagtg aaaaataaaa aaccatatta
      721 aaatactttc tcttccagaa cttctctaac atgtgttatt attaacaaag acaaatgtaa
      781 gcagggaaac tattccaagg taaatattgc gtcactaaat ggacattgga g
//
LOCUS       DAZPCS010000418          803 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZPCS010000418 DAZPCS010000000
VERSION     DAZPCS010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.426"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            46..803
                     /locus_tag="ACHUQ7_004357"
                     /pseudo
     CDS             46..803
                     /locus_tag="ACHUQ7_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS150 family transposase"
BASE COUNT          234 a          169 c          198 g          202 t
ORIGIN      
        1 tactgcaccc attttgttgg acgatgaaat ggaatagccc ctgatatgtc aaagccaaaa
       61 tacccttttg aaaagcgcct tgaagtcgtg aatcactact tcactactga tgatggttac
      121 aggatcatct cggcacgttt tggtgtcccc cgaacccagg tcaggacatg ggttgccctc
      181 tatgaaaaac atggagaaaa aggcttaatt cccaaaccta aaggtgttag tgctgatcca
      241 gagttgcgta ttaaggtcgt gaaagctgtg attgagcagc acatgtccct taatcaggct
      301 gctgctcact ttatgcttgc tggtagtggt tctgtagcca ggtggctgaa ggtttatgaa
      361 gagcgcggag aagctggttt acgcgcgctc aagattggca ccaaaagaaa cattgcaata
      421 tcagttgatc cagaaaaagc ggcatcagca ttggagctgt caaaagaccg acgcattgag
      481 gatcttgaaa ggcaagttcg atttcttgaa acgcggcttg tgtatctaaa aaagctgaaa
      541 gccttagctc atcccacgaa aaagtgaaag tactcaacga gctaaggcag ttttatcctc
      601 ttgatgagct tctcagggct gcggagatac cgcgcagtac gttttattac catctaaagg
      661 ctctcagcaa gcctgacaag tatgcggacg ttaaaaagcg tattggtgag atttatcatg
      721 agaatagagg ccgatacgga taccgtaggg taacgctgtc tcttcatcga gaagggaaac
      781 agattaacca taaagctgtt cac
//
LOCUS       DAZPCS010000419          797 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZPCS010000419 DAZPCS010000000
VERSION     DAZPCS010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..797
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.427"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            17..>797
                     /locus_tag="ACHUQ7_004358"
     CDS             17..>797
                     /locus_tag="ACHUQ7_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1137428.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLIAKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNYHRDFLN"
BASE COUNT          249 a          176 c          145 g          227 t
ORIGIN      
        1 taaaccggag tagtgtatgg ccaatctcta cgacttgaaa aagttcgact taaatcttct
       61 tgtcatattt gagtgtattt accagcattt aagcattagc aaagctgctg aatcactata
      121 tatcacccca tcagccgtga gccagtcgtt acaacgctta cgtgcgcaat ttaatgaccc
      181 tttattcatt cgctcaggaa aaggtatcgc gccgacgaca acagggttga atctgcatca
      241 ccatcttgag aaaaacctca aaggccttga acaaacaatt aatatagtca ataagtccga
      301 actaaagaaa aactttatta tctacggccc tcagctcatt tcttgctcta ataatagtat
      361 gctgatccgc tgcctacggc aagatgcctc tgtcgagatt gagtgccatg atatcctcat
      421 gtcagcagag aatgctgaag aactgttggt tcaccgcaaa gccgatctgg tgatcaccca
      481 aatgccagtg atcagccgtt ccgtcatttg catgccattg catacaattc gcaatactct
      541 aatttgtagt aataagcatc cgcgaattac tgacaatagc acttatgaac aaattatggc
      601 agaagagttc acccaattaa tagccaaatc agcaggagtt gatgatattc aaatggaaat
      661 agacgagaag tttatgaatc gcaaaatttc atttcgtggt tcctctttgc tgacaataat
      721 taatagcatt gcagttacag atttactggg aatagtcccc tatgaacttt ataactacca
      781 ccgtgacttt cttaatt
//
LOCUS       DAZPCS010000420          793 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZPCS010000420 DAZPCS010000000
VERSION     DAZPCS010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..793
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.428"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..>793
                     /locus_tag="ACHUQ7_004359"
     CDS             <1..>793
                     /locus_tag="ACHUQ7_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFR1137429.1"
                     /translation="ERFFFWQLRSFKLDVRGYARHFKVLNSCLFALPPIAEQERIVEK
                     VSSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQNTAELAENWARISEHFDTLFT
                     TEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKRIAQEKAQLVKEGKIKKQKPLP
                     PISDEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYLNLYQVAPDIIEKGTGRVISKR
                     TVKESGVKGPNSRFYKGQIVYSKIRPSLSKVFLAEYNGLCSADMYP"
BASE COUNT          261 a          156 c          175 g          201 t
ORIGIN      
        1 gtgagcgttt ctttttttgg cagttacgat catttaaatt agatgtaaga ggatacgcga
       61 gacattttaa agtattaaat agctgcttat ttgcattacc ccccattgca gaacaagagc
      121 gaatagttga aaaagttagc tctttgatgt ccctctgcga ccaactagaa cagcaatccc
      181 tgaccagtct ggacgcacat cagcaactgg ttgaaaccct gttgggaacg cttacagaca
      241 gccaaaacac cgcggaactg gctgaaaact gggcgcgtat tagcgagcat ttcgacaccc
      301 tatttaccac cgaagccagc gtggatgcgt taaaacagac cattctgcaa ctggccgtaa
      361 tgggcaaact tgtgccgcag gatccgaatg acgaacccgc ctctgaactg ctcaaacgta
      421 ttgcgcagga aaaagctcaa ttggtgaaag aagggaaaat aaaaaaacaa aaaccactgc
      481 cgccaattag cgatgaggaa aaaccgtttg aacttccgga agggtgggaa tggtgtcgta
      541 ttggtaatat agttaatatt aaatctgaat tagtatcacc aaaagattat ttaaatttat
      601 atcaagtagc acctgatatt attgaaaagg gcactgggag agtgatatca aagagaacag
      661 tgaaagaatc aggagtcaaa gggccaaata gtcgctttta taaaggacaa attgtttatt
      721 ctaaaatccg cccgtcatta tccaaagttt ttttagcaga gtataatggt ctttgcagtg
      781 ctgatatgta tcc
//
LOCUS       DAZPCS010000421          789 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZPCS010000421 DAZPCS010000000
VERSION     DAZPCS010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.429"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..341
                     /locus_tag="ACHUQ7_004360"
     CDS             <1..341
                     /locus_tag="ACHUQ7_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein mxiK"
                     /protein_id="HFR1137430.1"
                     /translation="TAMFVANWNLLPAVAYFIGSQESRLINHSEMVISYYGGKISKQG
                     EAAIRSGFWHLIAWKENISVGIYERINLLFNPIALEGNYTPVERNLSRLNEGMQYAKR
                     HFTGIQTSCL"
     gene            283..>789
                     /gene="mxiN"
                     /locus_tag="ACHUQ7_004361"
     CDS             283..>789
                     /gene="mxiN"
                     /locus_tag="ACHUQ7_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa type III secretion system linker protein
                     MxiN"
                     /protein_id="HFR1137431.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKK
                     KAICVIKEATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIISTLLEQWLSSLRNTVTELKVVLPKCNLALRKKLELDLHKYRSDV
                     KIILKYSEG"
BASE COUNT          272 a           96 c          175 g          246 t
ORIGIN      
        1 taaccgctat gtttgttgcc aactggaatt tacttccagc tgttgcttat tttatcggta
       61 gtcaagagtc tcggttaatt aatcatagtg agatggttat tagttattat gggggcaaaa
      121 tcagtaagca aggggaggcc gctattcgaa gtggtttttg gcatcttatt gcgtggaagg
      181 aaaatatatc tgttggtata tatgaaagaa taaacttatt atttaatcca attgctttgg
      241 agggaaatta tacgcctgta gaaagaaatt tgtcccgatt aaatgaaggt atgcaatatg
      301 caaaaaggca ctttaccggt atccagacat catgcctatg atggtgttgt tataaagaga
      361 attgaaaagg aattatgtaa aaccataaaa gatagagaca ctgagagtaa gaagaaagca
      421 atttgtgtta taaaggaggc tacgaaaaaa gctgaatcac tgcgtataga tgctgtatgt
      481 gatggttatc aaattggtat acagactgct tttgagcata ttattgacta tatttgtgaa
      541 tggaagttaa agcaaaatga aaatagaaga aatattgagg actatataac tagtttgctt
      601 tcagaaaatt tacatgatga gaggatcatt agcactttat tggagcagtg gcttagttcg
      661 ttgcgtaata cagtaacaga attaaaagtg gttttgccca agtgtaattt agcactgagg
      721 aaaaagttag aacttgatct ccataaatat cgctcagatg tcaagattat tttaaaatat
      781 tctgaaggg
//
LOCUS       DAZPCS010000422          772 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZPCS010000422 DAZPCS010000000
VERSION     DAZPCS010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..772
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.430"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..243
                     /locus_tag="ACHUQ7_004362"
     CDS             <1..243
                     /locus_tag="ACHUQ7_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR1137432.1"
                     /translation="ELKNNGIKAVIPRKSNEKMASDGRAQLDRDAYCNRNVVERCFGR
                     LKEYRRIATRYDKTARNYLAMVKLGCIRLFYQRLRN"
     gene            358..>772
                     /locus_tag="ACHUQ7_004363"
     CDS             358..>772
                     /locus_tag="ACHUQ7_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element IS630 family transposase"
                     /protein_id="HFR1137433.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPS"
BASE COUNT          206 a          198 c          201 g          167 t
ORIGIN      
        1 gagctgaaaa ataacggtat aaaggcagta atcccccgaa agtcaaatga gaaaatggca
       61 tcggatggac gtgcacagct tgatcgtgat gcgtactgca atcgtaatgt cgttgaacgg
      121 tgctttgggc ggctgaaaga atatcgccgc atcgccacgc gttacgacaa aacggcgaga
      181 aattacctgg cgatggtgaa actgggctgc atccgactct tttatcaacg cttacgtaat
      241 taagggacac agcctaaata gctgcgcgga atagtagatc cctttgaggg aacttagccc
      301 ggattgtgcg atctgatcaa tcgccaaatc aaaacaaatc accaaccgga ctgagcaatg
      361 ccgatcatag caccaatttc ccgtgacgaa cgacgcctga tgcagaaagc catccataaa
      421 acacacgata aaaattatgc ccgcagactg actgccatgc tgatgctgca ccggggcgac
      481 cgtgtcagcg acgttgccag aacgctctgc tgcgcccgtt cctctgttgg acgctggatt
      541 aactggttca cgcagtcggg tgttgaggga ctgaaatcat tacctgccgg gcgtgcccgt
      601 cgctggccgt ttgagcatat ctgcacactg ttacgtgagc tggtaaaaca ttctcccggc
      661 gactttggct accagcgttc acgctggagt acagaactgc tggcaataaa aatcaatgag
      721 ataaccggct gccagttaaa tgccggaacc gttcgccgct ggttgccgtc tg
//
LOCUS       DAZPCS010000423          759 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZPCS010000423 DAZPCS010000000
VERSION     DAZPCS010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.431"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..>759
                     /locus_tag="ACHUQ7_004364"
     CDS             <1..>759
                     /locus_tag="ACHUQ7_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFR1137434.1"
                     /translation="ERILTDRENGEKGDNSQDTSYARVGVKGETQINPEMTGYGQFEL
                     DLEASNRHNPDQTRLAYAGLSYKDFGSFDYSRNVGVAYDAEAFTDMFVEWGGDSWAGT
                     DLFMTNRTNGVATYRNTDFFGMVEGLNFALQYQGKNEGTGNYKANGDGHGLSATYTID
                     GFSFAGAYANSDRTDWQSGDGKGERAEVWALSTKYDANNVYAAVMYGESHNMNSDDGD
                     VVNKTQNFEAVLQYQFDFGLRPSIGYSYSKALDVA"
BASE COUNT          211 a          155 c          184 g          209 t
ORIGIN      
        1 gagcgcattc tgaccgaccg tgaaaatggt gaaaaaggcg ataattctca agatacctct
       61 tatgcccgtg taggcgtgaa aggtgaaact cagatcaacc ctgaaatgac tggttacggt
      121 cagtttgaac tagacctgga agcatcaaac agacacaacc ctgaccaaac tcgtttggca
      181 tatgctggtt tgagttacaa agattttggt tcattcgact acagtcgtaa tgtcggtgtc
      241 gcgtatgatg cagaagcatt tactgatatg tttgttgagt ggggcggtga ctcatgggct
      301 ggtactgacc tgttcatgac caaccgtacc aacggtgttg caacctatcg taacacagat
      361 ttctttggca tggttgaagg tctaaatttt gctctgcaat atcagggtaa aaatgaaggt
      421 acaggcaact acaaagcaaa tggcgatggc catggtttat ctgcaactta cactattgac
      481 ggtttctcat ttgctggtgc ttatgcaaac tctgaccgta ctgattggca gtctggtgat
      541 ggtaaaggcg aaagagccga agtttgggcg ttgtcaacaa aatatgacgc taacaatgtt
      601 tatgctgctg tgatgtacgg tgaatctcac aacatgaatt ccgacgacgg tgatgttgtg
      661 aacaaaacac aaaacttcga agctgttctg caatatcagt tcgactttgg tctgcgtcca
      721 tccatcggtt actcctattc taaagcactg gacgtagca
//
LOCUS       DAZPCS010000424          734 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZPCS010000424 DAZPCS010000000
VERSION     DAZPCS010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..734
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.432"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..636
                     /locus_tag="ACHUQ7_004365"
     CDS             <1..636
                     /locus_tag="ACHUQ7_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFR1137435.1"
                     /translation="PLSKTEFNALMESEKGQSQNLWKFAVYSGLRHGELAALAWEDVD
                     FEKGIVNVRRNLTILDMFGPPKTNAGIRTVALLQPALEALKEQYKLTGHHRKSEITFY
                     HREYGRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGTRWNAAVKRAGIRRRNPYHTRHT
                     FACWLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARLS"
BASE COUNT          236 a          150 c          182 g          166 t
ORIGIN      
        1 ccgttatcta aaacagaatt caatgcatta atggaaagtg aaaaaggaca gagccaaaac
       61 ttgtggaaat ttgccgttta ctccgggctt cgtcacgggg aactggcagc tctggcgtgg
      121 gaggatgtgg atttcgagaa gggaattgtg aatgtcagaa gaaacctgac gatacttgat
      181 atgttcggtc ccccaaaaac aaatgccggg atccggacgg tagcattact gcagccggct
      241 cttgaagcac tgaaggagca atacaaactg accgggcatc atcgcaaaag cgaaatcact
      301 ttttatcatc gggagtacgg cagaactgaa aaacaaaaac tgcattttgt tttcatgccc
      361 agggtgtgta acgaaaaaca gaaaccttat tactcggtaa gcagtttggg tacaagatgg
      421 aatgcagcag taaaacgtgc tggtattcgc cgccgtaatc cgtaccatac gcgacatact
      481 tttgcctgct ggctgttgac ggcaggagcg aacccggcat ttatagccag ccagatgggg
      541 catgaaactg cgcaaatggt gtatgaaatt tacggtatgt ggattgatga catgaacgac
      601 gaacagatag ccatgttgaa tgcgcggtta tcgtagttgc aaagtttgcc cccaatttgc
      661 cccatttagt accagagaac tgaaataatg caagaaaatc aacaaattac aaagaaagaa
      721 caatacaacc tgaa
//
LOCUS       DAZPCS010000425          720 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZPCS010000425 DAZPCS010000000
VERSION     DAZPCS010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..720
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.433"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            51..>720
                     /locus_tag="ACHUQ7_004366"
     CDS             51..>720
                     /locus_tag="ACHUQ7_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR1137436.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPADLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALE"
BASE COUNT          236 a          122 c          161 g          201 t
ORIGIN      
        1 tgttaacgat ttaccagtaa tgtaaataaa ttttcgagga gatcattcca gtgggacgta
       61 aatgggccaa tattgttgct aaaaaaacgg ctaaagacgg tgcaacgtct aaaatttatg
      121 caaaattcgg tgtagaaatc tatgctgctg ccaaacaagg tgaacccgat ccagaattaa
      181 acacatcttt aaaattcgtt attgaacgtg caaagcaggc acaagttcca aagcacgtta
      241 ttgataaagc aattgataaa gccaaaggcg gcggagatga aacgttcgta cagggacgtt
      301 atgaaggctt tggtcctaat ggctcaatga ttattgctga gacattgact tcaaatgtta
      361 accgtacgat tgctaacgtt cgcacaattt tcaataaaaa aggcggcaat atcggagcgg
      421 caggttctgt cagctatatg tttgacaata cgggtgtgat tgtatttaaa ggaacagacc
      481 ctgaccatat ttttgaaatt ttgcttgaag ctgaagttga tgttcgtgat gtaactgaag
      541 aagaaggtaa tattgttatt tatactgaac ctgctgacct tcataaagga atcgcggcgc
      601 taaaagcagc tggaatcact gagttctcaa caacagaatt agaaatgatt gctcaatctg
      661 aagttgagct ttccccagaa gatttagaaa tctttgaagg gcttgttgat gcccttgaag
//
LOCUS       DAZPCS010000426          714 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZPCS010000426 DAZPCS010000000
VERSION     DAZPCS010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.434"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..>714
                     /gene="yfdF"
                     /locus_tag="ACHUQ7_004367"
     CDS             <1..>714
                     /gene="yfdF"
                     /locus_tag="ACHUQ7_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YfdF"
                     /protein_id="HFR1137437.1"
                     /translation="NSSVTYIDIGSNDTDHITIGIDNQEPIELPANYKDKELVRTIIN
                     DNIVEKTHDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEFDESGKSEPTDLF
                     TWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGDSLTQYCSENARS
                     ASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRAIRYNFQVDDAKF
                     RRDNVKEIISTLFANKVDV"
BASE COUNT          261 a          124 c          138 g          191 t
ORIGIN      
        1 taattcctca gttacttata ttgatattgg ctccaatgat acagaccata ttactattgg
       61 tatcgacaac caagaaccaa tagaattacc tgcgaactat aaagacaaag aactcgtccg
      121 tactatcatt aatgacaaca tagttgagaa gactcatgat atcaataaca aggaaatgat
      181 cttcagcgca ttaaaagaaa tatatgatgg agatcctggt tttatcttcg ataagatatc
      241 acacaaactc agacatacgg taacggaatt tgatgagagc gggaaaagcg aaccaactga
      301 cttatttacc tggtacggta aagataaaaa aggcgactct ctcgctattg taattaaaaa
      361 taaaaacgga aatgattact tatctctcgg ttactacgat caggacgact accacattca
      421 aagaggaatt cgtattaatg gtgatagtct cacccaatat tgtagtgaaa acgccaggag
      481 tgcttcagcg tggtttgaaa gcagtaaagc tatcatggca gaatcatttg caactggttc
      541 cgatcatcag gttgtaaacg agctcaacgg ggaaagactg agagaaccta acgaagtttt
      601 taaacgttta ggtcgagcaa taagatataa ttttcaagtg gacgatgcaa aatttagacg
      661 cgataatgta aaagaaataa tttctacttt attcgctaac aaagttgacg ttga
//
LOCUS       DAZPCS010000427          709 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZPCS010000427 DAZPCS010000000
VERSION     DAZPCS010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.435"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..591)
                     /gene="fimE"
                     /locus_tag="ACHUQ7_004368"
     CDS             complement(<1..591)
                     /gene="fimE"
                     /locus_tag="ACHUQ7_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HFR1137438.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREE"
BASE COUNT          205 a          178 c          144 g          182 t
ORIGIN      
        1 ctcttctctt tttaattttt cgtttatgag attatttctt tcccataatc cggcaaaacg
       61 agcagcatta ctggcggtat aacgcacagt atggcgaata tttcgatgcc cgagataatc
      121 ctgaattaaa cgggtatctg caccacgctc cgccagttca taaccgcaag catgccttaa
      181 catatgagga tgagtctgcg ttacggttcc agcttcaata ccggcatcgc gaataatgcg
      241 ataggcctgc tggcgagaaa gccgactccc gcggcgagaa ataaatatag cgtcggtccg
      301 gtcagcgcct ttccagttag cacgttcctg ggtccagcgt tccacggctt cacgctcatc
      361 aaaacgtaac gggtgaacgg tagaaaatcc gttcttcagt cggcgaatat ttattcgacc
      421 ttcattaagg tcaaggtcct gataatgcag atcaagcagt tcactaatac gcatcccatg
      481 ccgatatgcc aacagaataa gacaataatc tctggctccc gttgccccgt aacaaaccgc
      541 ctgcatcatg gcctgaactt ctttaccggt aagataacga cgtttactca caatagtagt
      601 actcctaact gagatatatt tgaatgaata cctataggaa acctcaatcg gtcaaaatta
      661 gcccaataga taaaaagata accacacacg aagttatgtg gctatcagt
//
LOCUS       DAZPCS010000428          704 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZPCS010000428 DAZPCS010000000
VERSION     DAZPCS010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.436"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..443)
                     /locus_tag="ACHUQ7_004369"
     CDS             complement(<1..443)
                     /locus_tag="ACHUQ7_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFR1137439.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALAFQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPD"
BASE COUNT          214 a          172 c          131 g          187 t
ORIGIN      
        1 tcatctggat aatcggcatg agcaataaca tgcttgccgg tgcgactgtg tacttcaaga
       61 ataaagggca aatgctgaca tttttccagc gccgttttcg cttgtttctg ttgattatct
      121 gccagcgcaa taaaccagtc gccgccattc atcaaccaca aagacatctg ctggaatgcc
      181 agcgcatcca tcgccatctg ttcatgattg cctcttaccg cacgaaccca atgttgttcc
      241 agtaactgca gacaacgtaa actttgcggc ccacgatcga taacgtctcc cactgagata
      301 agtaaatctc gccacggatc aaaacgacaa tgccataatt tgcggcgcaa ctgctcaaga
      361 caaccgtgta tatcgccaga aagccagata tgtcgccatt gatgacccgc aattctctga
      421 taaacgggcg caggctgttt catcaatatt ttcctcccgc gctaaagatc acataatctt
      481 aacaagaatg ttaaaaaacg ctggactcag acagtagagt gtgtgttatg gttgactata
      541 aagtcagcga aggaaatgct tctggctttt aacagataaa aagagaccga acatgattcc
      601 tgttttcgtc aacaaacaat aaaacccttt aaaattaatg cgttgaataa atttacgttc
      661 atcttttaat ccctgacacg taccattgca tattaataca ttca
//
LOCUS       DAZPCS010000429          698 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZPCS010000429 DAZPCS010000000
VERSION     DAZPCS010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.437"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..351)
                     /locus_tag="ACHUQ7_004370"
     CDS             complement(<1..351)
                     /locus_tag="ACHUQ7_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HFR1137440.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVEN"
     gene            427..576
                     /locus_tag="ACHUQ7_004371"
                     /pseudo
     CDS             427..576
                     /locus_tag="ACHUQ7_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          208 a          130 c          130 g          230 t
ORIGIN      
        1 gttttcaaca aaataaataa tagcaggcac attaatagcc ttttcttctt cactcactga
       61 aacataattt ttaatttcat ccggaaaagc aaagtgatga taatccacgt gaccactact
      121 tttgttaaac tgtgctatcc agtaggaagc taaatgtcga cagacaataa gtcttccaga
      181 gtgatcggta acttggcaat ttagatttaa taaaaaactc ctcttgtcag aaaaatatgg
      241 caattgtttt gagtgcaata gagactggat tgtctcaaca gagacgttgt ctttatttat
      301 catattttgc aaacatattt tccttgatgg gattaaattt actgatggca tatgtatata
      361 ttcctttcat tgatgggttg tgacgcagac ttagtgttgt tgtactcttt gccctttgga
      421 tgattgatgg caaagattga tgtggtttgt caccgttact ctgaaactca ggggggtatc
      481 cggaacggcc attccagttc aggggggctg ctctatcgct gtaagcagtg cctgaagacc
      541 ttccagttcg actaccgcta caacggggcg aaacccatca aactttccaa ctattatcac
      601 attgagccat actgacaata aacaatatat cgatattttg tgcaaatact gccttttgct
      661 aaacgatatt caattttgat taaatatatt tattgtca
//
LOCUS       DAZPCS010000430          697 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZPCS010000430 DAZPCS010000000
VERSION     DAZPCS010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.438"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..>697)
                     /locus_tag="ACHUQ7_004372"
     CDS             complement(<1..>697)
                     /locus_tag="ACHUQ7_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR1137441.1"
                     /translation="QYLLSLVPYCPWQHIVFTLPCQYWSLVFHNRWLLAEMSRIAADV
                     ILEICHQADVEPGIFTVIHTWGRDQQWHPHIHLSTTAGGVTSGHTWKNLHFYARKVMS
                     MWRYRITRLLSRKYPYLVMPDALAAEGSSKREWNRFLDTHYRRGWNVNVSRVMDNATH
                     VAVYFGSYLKKPPVPMSRLEHYAGQDEIGLRYNSHRTKREEYLLMSGDESMERFSWHV
                     ADKGFRMVRYYVF"
BASE COUNT          165 a          205 c          167 g          160 t
ORIGIN      
        1 aaaaaaacgt agtacctcac catacggaac cccttatccg ccacatgcca ggagaacctt
       61 tccatggact catctccact catcaacagg tattcttccc gttttgttcg gtgactgttg
      121 taacgcagac cgatttcatc ctgaccggca taatgctcca gacgactcat cggcaccggt
      181 ggctttttca ggtaagagcc aaagtacacc gccacatggg tggcattatc cattacccgg
      241 gatacgttga cattccagcc acggcggtaa tgcgtgtcca ggaagcgatt ccattcccgt
      301 ttactgcttc cttctgctgc cagcgcatcc ggcatcacca ggtaagggta tttccgtgac
      361 agcaaccgtg ttatccggta gcgccacatg ctcatcacct tacgggcgta aaaatgaaga
      421 tttttccagg tgtggcccga cgtcacacca ccggcagttg tcgataaatg gatatgcgga
      481 tgccactgct ggtcacgccc ccatgtgtgg atcaccgtga atatccccgg ctccacatct
      541 gcctgatggc agatttccag tatcacatcc gctgcaatgc ggctcatctc tgccagtaac
      601 caccggttgt ggaacaccag ggaccagtac tggcagggaa gtgtgaacac aatatgctgc
      661 cacgggcagt aggggaccag gctcagcaga tactgta
//
LOCUS       DAZPCS010000431          696 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZPCS010000431 DAZPCS010000000
VERSION     DAZPCS010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.439"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            32..493
                     /locus_tag="ACHUQ7_004373"
     CDS             32..493
                     /locus_tag="ACHUQ7_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFR1137442.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKSTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(534..>696)
                     /locus_tag="ACHUQ7_004374"
     CDS             complement(534..>696)
                     /locus_tag="ACHUQ7_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFR1137443.1"
                     /translation="ILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGL
                     AARQHVKKS"
BASE COUNT          204 a          152 c          160 g          180 t
ORIGIN      
        1 agtttcttga ttaaataaaa tggagtttta catgaaggct ttcaataagc tgttttccct
       61 cgttgttgca tctgttctgg ttttctctct tgctggctgc ggtgacaaag aagaatcgaa
      121 gaaattcagc gccaatctga acggcactga aattgccatt acctatgtct acaaaggtga
      181 caaggtgctt aagcaatctt ctgaaaccaa aattcaattt gcctccattg gtgcaaccac
      241 caaagaagat gctgccaaga cacttgagcc gttaagcgcc aaatacaaaa acatcgcggg
      301 tgttgaagaa aaatcaacct ataccgatac ctacgcgcag gaaaacgtga ctatcgatat
      361 ggaaaaagtg gattttaaag ccctgcaggg tatttcagga atcaacgttt ctgctgaaga
      421 tgccaaaaaa ggtatcacta tggcgcaaat ggaactggtg atgaaagccg ctggttttaa
      481 agaagtgaaa taatctgtcg gcggccatgt ttcgcatggc cgccataccc gtcttagctt
      541 ttcttcacat gctggcgcgc tgccagtcca cgcagaaaat aacgtaaaaa ttgatcgccg
      601 cattcgcgga agtttttatg atccggtgcg cgcatcatcg ctgtaatttc cggcatcgaa
      661 acgcggaact gctgttcggt gaggatagcc agaatg
//
LOCUS       DAZPCS010000432          696 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZPCS010000432 DAZPCS010000000
VERSION     DAZPCS010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.440"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..673
                     /locus_tag="ACHUQ7_004375"
     rRNA            <1..673
                     /locus_tag="ACHUQ7_004375"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          175 a          164 c          206 g          151 t
ORIGIN      
        1 ttaagtcgac cgcctgggga gtacggccgc aaggttaaaa ctcaaatgaa ttgacggggg
       61 cccgcacaag cggtggagca tgtggtttaa ttcgatgcaa cgcgaagaac cttacctggt
      121 cttgacatcc acggaagttt tcagagatga gaatgtgcct tcgggaaccg tgagacaggt
      181 gctgcatggc tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc
      241 aacccttatc ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata
      301 aactggagga aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca
      361 cgtgctacaa tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa
      421 gtgcgtcgta gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta
      481 atcgtggatc agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac
      541 accatgggag tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac
      601 tttgtgattc atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg
      661 gatcacctcc ttaccttaaa gaagcgttct ttgtag
//
LOCUS       DAZPCS010000433          693 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZPCS010000433 DAZPCS010000000
VERSION     DAZPCS010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.441"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            1..525
                     /locus_tag="ACHUQ7_004376"
                     /pseudo
     CDS             1..525
                     /locus_tag="ACHUQ7_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            589..>693
                     /locus_tag="ACHUQ7_004377"
     CDS             589..>693
                     /locus_tag="ACHUQ7_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR1137444.1"
                     /translation="MSGKRYPEEFKTEAVKQVVDRGYSVASVATRLDIT"
BASE COUNT          193 a          143 c          166 g          191 t
ORIGIN      
        1 tgtgctgatc gtgcggatga ttactaccat gaccgtctgg cgctgactgt tgataattca
       61 acgatcgacg acaactacga acactacaca tataacggca cttacaataa tgccgctgat
      121 actcatgttg tagacgttta caacattggt acggctatta ctctggatca ggaagttgat
      181 ctgtccatca ctaataactc tcacgttgcg ggtattacgt tgactcaggg ttatgagtgg
      241 gaagatattg acgacaacac tgtcagcact ggcgtaaaca gcagcgaagt gtttaataac
      301 accatcactg ttaaagattc taccgtaacc tctggttcat ggactgatga aggtactact
      361 ggttggttcg gtaatacagg taatgccagc gattatagtg gtaaatctaa ctttgtcacc
      421 gtagatactg acggagatgg tgttgctgat agcactattg caagctggga tgatgttgca
      481 ttagctgtag ttgcacatcc gaatgttgat aatgctatgc agacctgaat cgccacgggt
      541 ttaacagaca cctcagagtc atttaagatg gcttaaagag aggtgcccat gagcggtaag
      601 cgttatcccg aagagtttaa aactgaagca gtcaaacagg ttgttgatcg cggttattct
      661 gttgccagcg ttgcaacacg tctcgatatc acc
//
LOCUS       DAZPCS010000434          685 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZPCS010000434 DAZPCS010000000
VERSION     DAZPCS010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.442"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            52..685
                     /locus_tag="ACHUQ7_004378"
                     /pseudo
     CDS             52..685
                     /locus_tag="ACHUQ7_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
BASE COUNT          205 a          172 c          173 g          135 t
ORIGIN      
        1 gccacctgaa cagaggtgat atgctcacct cagaacaaca caggtgctcc aatgaaaaaa
       61 agaaatttta gcgcagagtt taaacgcgaa tccgctcaac tggttgttga ccagaaatac
      121 acggtggcag atgccgccaa agctatggat gttggccttt ccacaatgac aagatgggtc
      181 aaacaactgc gtgatgagcg tcagggcaaa acaccaaaag cctcccccat taccccggaa
      241 caaattgaaa tccgtgagct caggaaaaag ctacaacgca ttgaaatgga gaatgaaata
      301 ttaaaaaagg ctaccgcgct cttgatgtca gactccctga acagttctcg ataatcggga
      361 aactcagagc gcattatcct gtggtcacac tctgccatgt gttcggggtt catcgcagca
      421 gctacagata ctggaaaaac cgtcctgaaa aaccagacgg cagacgggct gtattacgca
      481 gtcaggtact tgagctacat ggcatcagcc acggttcggc cggagcaaga agcatcgcca
      541 caatggcaac ccggagaggc taccagatgg gacgctggct tgctggcagg ctcatgaaag
      601 agctggggct ggtcagctgt cagcagccga ctcaccggta taaacgtggt ggtcatgaac
      661 atgttgctat ccctaactac cttga
//
LOCUS       DAZPCS010000435          684 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZPCS010000435 DAZPCS010000000
VERSION     DAZPCS010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.443"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(176..388)
                     /gene="cspH"
                     /locus_tag="ACHUQ7_004379"
     CDS             complement(176..388)
                     /gene="cspH"
                     /locus_tag="ACHUQ7_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFR1137445.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
BASE COUNT          209 a          130 c          142 g          203 t
ORIGIN      
        1 agtttcaagt ggtactataa ctggctgtta aatggcattt ataactatta ggtgcagaga
       61 tattcgctta aatggattag tttcttagtg gaaaagtaac tgatgttata atgtatggtg
      121 ttgactattg aactattcca ataaaaccag tcagctttaa acaagcagcg tcatattaag
      181 agagataaac atttgccgct gttggtcctc gcaggccatt tacgcggcaa aattccacgc
      241 gtaatcctgg tataagcact tctgcgtcgc ggggagtgaa tgcggaaata tggacctgga
      301 cttctttacg accgtcggag gggataatga atcctttgcc gcttttgcga tcaaaggttt
      361 tgacaattcc tgtcatttta cgggacaaac aaattcctta ctgaaaatac tgcgctgcac
      421 tatacggggt taataaaata aagccagcga tatttaagac cgccggacgg ctaaaataaa
      481 atttgcttaa tctcaattat catgccttaa tagctgcgtc ggtttgaaag acagacagca
      541 tacaaagtag tttactaaag cagttctcat tatcaggcat tatccccttc ttttgagtct
      601 ctctcctgaa cactaagtgg tttctgtatt aaagccctgt ttgccgaaag gcccaaaatg
      661 aaggaagtaa aatatgtcta ataa
//
LOCUS       DAZPCS010000436          682 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZPCS010000436 DAZPCS010000000
VERSION     DAZPCS010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..682
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.444"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(19..149)
                     /locus_tag="ACHUQ7_004380"
                     /pseudo
     CDS             complement(19..149)
                     /locus_tag="ACHUQ7_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611212.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            147..230
                     /gene="tnpB"
                     /locus_tag="ACHUQ7_004381"
                     /pseudo
     CDS             147..230
                     /gene="tnpB"
                     /locus_tag="ACHUQ7_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          187 a          137 c          174 g          184 t
ORIGIN      
        1 ttcgggtggc gcttccaatc agtagaatat gtgtttttca atgaaggagc cgatgacttt
       61 tcgtggattt ccacggaacg cgagaagcag attgttttac gtgccagccg cttgatgcgg
      121 gtgcggaggg tcaggttgtt acgttcaaga tatagcatgt gctggatgaa gatccctttt
      181 ctggtcacct gtttatcttt cgtggccgac ggggtgacac tgttaaaatg taatgaacgc
      241 atcccacaca attaaacacg gagtagtatg atggttaaaa gggtgaaact ggcaggtgtg
      301 gagcctcgca gctttccggc atcatactgc ccaatacggg atgcataaag cgtcctcaga
      361 ctggagactc cacaaatgaa atatacaccg gttggcgttg atatggcaaa acatgtcatt
      421 cagattcact tcatcaatga gcacatgtca gcgccagtga tataagacgg taatggggat
      481 gcggacgatt tcctggactc tcagaagatg aaacatgttt actgaagaac aaaaaattcg
      541 cgctattgag ctttatttca aatatggtaa gaagcttgag gtgtactggc aatagcggac
      601 accaccattt gttctttttc taagcagtca tctgataata ttttatcctg aaggctgccg
      661 gggagatatt ccccagacga ga
//
LOCUS       DAZPCS010000437          680 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZPCS010000437 DAZPCS010000000
VERSION     DAZPCS010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..680
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.445"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..252)
                     /locus_tag="ACHUQ7_004382"
     CDS             complement(<1..252)
                     /locus_tag="ACHUQ7_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFR1137446.1"
                     /translation="MGLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIK
                     RWIDNGVQVSKVKILLSNENVDVQNVWRDQQETLLTYLQS"
     gene            complement(273..>680)
                     /locus_tag="ACHUQ7_004383"
     CDS             complement(273..>680)
                     /locus_tag="ACHUQ7_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1137447.1"
                     /translation="HRALSEHKTLSVSWSHYRSTVHKCTFVFSPQVIKQRQFSGPHYK
                     VDSIRESWTSILKRAGLRHRKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQMVFNV
                     YGAWMKDNNHEQIELLNRRLSESVPCMPHKKAG"
BASE COUNT          155 a          156 c          153 g          216 t
ORIGIN      
        1 gctttgcagg taagtcagta atgtttcttg ctgatcgcgc cagacgttct gcacatcaac
       61 attttcatta ctgagcagta ttttaacttt gctgacctgc acgccgttgt cgatccagcg
      121 tttgatctcg cggatccggt caatatcggc atcgttgaac agccgatgac cgccgtctgt
      181 ccgttgcggt ttcagcaatc cgtaacgcct ctgccacgcg cgtaacgtga cagggattaa
      241 aggtaaacca attgattttg caagttttta ttttaccccg ctttcttatg gggcatgcat
      301 gggacacttt cagatagtct tctgttaaga agttctatct gttcgtgatt gttgtctttc
      361 atccatgctc cgtaaacatt gaataccatt tgtgcgtttg tgtggcccat ctggcttgcg
      421 ataaaactag gattagctcc tgcggcaagt gaccagcatg cataggtatg cctggattga
      481 tacgattttc tgtgtctcag acctgcgcgt tttaagatac ttgtccatga ctccctgatg
      541 gagtcaacct tatagtgcgg tccggaaaac tgccgctgtt ttattacctg aggactaaaa
      601 acaaaagtgc atttatgcac agtacttcgg taatgactcc aacttactga tagtgtttta
      661 tgttcagata atgcccgatg
//
LOCUS       DAZPCS010000438          671 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZPCS010000438 DAZPCS010000000
VERSION     DAZPCS010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.446"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..>671
                     /locus_tag="ACHUQ7_004384"
     CDS             <1..>671
                     /locus_tag="ACHUQ7_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFR1137448.1"
                     /translation="AATGGLIAVSELAKRVSGKTNRKLVATMLSLAVAGTVNAANIDI
                     SNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGAAASGAATAIG
                     GSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVVETVGATPAET
                     NPTTYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAYSHDLLSASLF
                     EVTQW"
BASE COUNT          211 a          135 c          141 g          184 t
ORIGIN      
        1 gctgccactg gcggactcat tgctgtttct gaattagcga aaagagtttc tggtaaaaca
       61 aaccgaaaac ttgtagcaac aatgttgtct ctggctgttg caggtacagt aaatgcagca
      121 aatattgata tatcaaatgt atgggcgaga gactatcttg atcttgcaca aaataaaggt
      181 attttccagc ccggagcaac agacgtaaca atcactttaa aaaacggaga taaattctct
      241 ttccataatc tctcaattcc ggatttttct ggtgcagcag cgagtggcgc agctaccgca
      301 ataggaggtt cttatagtgt tactgttgca cataacaaaa agaaccctca ggccgcagaa
      361 actcaggttt acgctcagtc ttcttacaag gttgttgaca gaagaaattc caatgatttt
      421 gagattcaga ggttaaataa atttgttgtg gaaacagtag gtgccacccc ggcagagacc
      481 aaccctacaa catattctga tgcattagaa cgctacggta tagtcacttc tgacggttca
      541 aaaaaaatca taggttttcg tgctggctct ggaggaacat catttattaa tggtgaatcc
      601 aaaatctcaa caaattcagc atatagccat gacctgttaa gtgctagtct atttgaggtc
      661 acccaatggg a
//
LOCUS       DAZPCS010000439          668 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZPCS010000439 DAZPCS010000000
VERSION     DAZPCS010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.447"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..>668
                     /locus_tag="ACHUQ7_004385"
     CDS             <1..>668
                     /locus_tag="ACHUQ7_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110-like element ISSfl4 family transposase"
                     /protein_id="HFR1137449.1"
                     /translation="ARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIRG
                     LLTQIHPAPERVLGPRLEHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKRL
                     AADIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRDEVALEVEQRVLAHPLYPV
                     LTSMPGVGVRTAARLLTEVACRAFASAAHLAAYAGLAPVTRRSGSSIRGEHPSRRGNK
                     ALKR"
BASE COUNT          133 a          230 c          177 g          128 t
ORIGIN      
        1 ctgcccgtac cctgcctcac gcgctacgca cgctgaaact ggctgacgag caaatcgccg
       61 aactctccat gctctgcggc ttcgatgatg atcttgccgc acagacaacg caggccagca
      121 accgtatccg cggccttctg acccagatac atccggcacc ggagcgcgtt ctcggtccga
      181 gacttgagca cccggcggta ctcgatcttc tccagcgata tccctcacca gaaaaactcg
      241 cttcgctggg tgagaagaag ctggcagccc agctctgcaa acttgcgcct cgtctgggta
      301 aacgccttgc agcagacata gctcaggcac tggccgaaca aaccgtcgtc gttcccggca
      361 cgaatgccgc tgccgtagta ctgccacgtc tggcactcca gctcatcacg ctgcgtaagc
      421 aaagagacga ggtggcgctt gaggtagaac agcgagttct tgctcaccct ctttacccgg
      481 tcctgaccag tatgcccgga gtcggtgtca ggaccgcagc cagactcctc accgaggtcg
      541 cctgccgcgc cttcgcctct gccgcacatc tcgctgctta tgctggcctt gcgccggtaa
      601 ctcggcgatc cggctcgtca atacgcggtg agcatccctc gcgacggggt aataaagctc
      661 tcaaacgg
//
LOCUS       DAZPCS010000440          662 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZPCS010000440 DAZPCS010000000
VERSION     DAZPCS010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.448"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(88..>662)
                     /locus_tag="ACHUQ7_004386"
     CDS             complement(88..>662)
                     /locus_tag="ACHUQ7_004386"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFR1137450.1"
                     /translation="PEEKIIFYAGRIVPDKGILLLMQSFEKLAAAHKNLKLVVIGDYT
                     EMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMYCYYPLADLVVIPSQFQEPFCM
                     VAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSISSDILKTLANPELTAV
                     AKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
BASE COUNT          199 a          147 c          110 g          206 t
ORIGIN      
        1 aaatttatta ggcgtttacg ccggcttatt attagcgatg aaaaacaagt aaaaaataaa
       61 aaaggctgca taatgcggcc tttttattta ttcaaaccaa ttatgaataa cctcttcgaa
      121 tctttgagtt acgccatccc aactgtagtg atcaaacaca aagtcctgac cttgctttgc
      181 aactgctgtt aattcaggat tcgctaacgt ttttaaaata tcgctgctta tggagtctgc
      241 agtcataggt tcttttaaat ggaatcccgt agtattttct ttgacaaatt ccgtcatccc
      301 cccacgcgta ctcactaaaa caggcttacc agcacccata gcttcaattg ccaccatgca
      361 aaatggttcc tggaactggg aaggaatgac gactaaatct gccagtgggt aataacaata
      421 catcttttca ggcggtatgc ttcctaacat aatgcaacga tccttcaaac gttttgcaat
      481 ttccctgaca ttgcgttgat atgcaccttt gtcactttta ctcatttctg tataatcacc
      541 aatcacaact aattttaaat ttttgtgagc agcggccaat ttttcgaatg attgcatcaa
      601 cagcaatatg cctttgtcag ggacgatacg cccagcataa aaaataattt tctcttcagg
      661 ag
//
LOCUS       DAZPCS010000441          656 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZPCS010000441 DAZPCS010000000
VERSION     DAZPCS010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.449"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..>656
                     /locus_tag="ACHUQ7_004387"
     CDS             <1..>656
                     /locus_tag="ACHUQ7_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1137451.1"
                     /translation="VLVPELGLVVLKPGRESMPVFHNTRVLVEPEPKSMRNLPSGVVP
                     AVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHHSETVIHR
                     YGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERELSLAELS
                     WWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQRTKNLAPL
                     P"
BASE COUNT          144 a          179 c          189 g          144 t
ORIGIN      
        1 gttctggttc cggaactcgg gctggtggtc cttaagcccg gtcgtgaatc catgccggta
       61 ttccacaata cccgggtact ggtggagccg gaaccgaaaa gcatgcgtaa tctgccgtcc
      121 ggggtcgttc ctgccgttcg ccagccgcta gtggaagaca aaacattgct gccgtttttc
      181 agtaacgcac gggtgattcg tgctgctggt ggtgctggcg cattgtctga ctggctgttg
      241 cgccatatta aatcctgcca gtggccacac ggcgattatc atcacagcga aaccgtcatt
      301 caccgttatg gtaccggcgc aatggtattg tgctggcact gcgacaacca gctgcgtgac
      361 cagacatccg aatcactcga gcaacttgct catcaaaacc tgtcagcatg gatgattgac
      421 gtcatcggtc acgcaataag cggtacgcag gagcgtgaat tatctctggc tgaattatcc
      481 tggtgggcgg tccgcaatca ggtggcggac gcgctaccgg aagcggtatt acgtcgttcg
      541 ctggggttgc gtgcggaaaa aatccgctca atgtaccgtg aaagcgacat cgtgccggga
      601 gagcagaccg ccaccagcat actgaagcag cgcacaaaaa atcttgcgcc gctgcc
//
LOCUS       DAZPCS010000442          656 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZPCS010000442 DAZPCS010000000
VERSION     DAZPCS010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.450"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(151..>656)
                     /locus_tag="ACHUQ7_004388"
     CDS             complement(151..>656)
                     /locus_tag="ACHUQ7_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000211421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFR1137452.1"
                     /translation="PVITTELLAQLYGTEPVRIRQNHHENKVRFVEGKHFFKVVGNDL
                     KELRVALNYSQNPVSPKARSLILWTERGAARHGKMLETDQAWDVFEKLEDCYFSQRDP
                     STPVSCQKSYDTRVLCYQRGGVTVSTIQLRDDDIVISLESWLELARANGWFVVRRDKL
                     VERLMQL"
BASE COUNT          173 a          160 c          144 g          179 t
ORIGIN      
        1 gtttaacgcc ctgtgccatt tttcaggcac aaaaaaaccc gcataaagcg ggttctttca
       61 agtgtccatg tctgctattc gcctcgcgat acagctttgc gaagcttacc gtaattgaag
      121 cagtttatgt gcaaaaatgc aagaactttt ttaaagctgc atcaaccttt ccaccagttt
      181 atctctgcga acaacaaacc aaccattggc tctcgccagt tccagccatg actcaaggga
      241 aataacaata tcatcatccc gtaactgaat tgtggaaaca gtgacaccgc ctctctgata
      301 acagagaact cgcgtgtcgt aacttttctg gcatgaaact ggcgttgacg gatccctttg
      361 actgaaatag cagtcttcca gtttttcgaa cacatcccac gcctgatcgg tttcgagcat
      421 tttgccgtgg cgtgctgcgc ctcgttctgt ccagaggatg agggagcggg ctttgggtga
      481 aactggattt tgtgagtagt ttaaagcgac ccgcaattct ttaaggtcat taccaacaac
      541 tttgaaaaag tgtttccctt caacgaagcg tactttgttc tcatgatgat tctggcgaat
      601 acgcaccggc tcagtgccgt aaagctgcgc caaaagttcg gtggtaataa caggaa
//
LOCUS       DAZPCS010000443          641 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZPCS010000443 DAZPCS010000000
VERSION     DAZPCS010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.451"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..143)
                     /gene="csgC"
                     /locus_tag="ACHUQ7_004389"
     CDS             complement(<1..143)
                     /gene="csgC"
                     /locus_tag="ACHUQ7_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="involved in autoagglutination of curliated cells;
                     not involved in production of curli fibers; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HFR1137453.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLR"
     gene            complement(202..567)
                     /gene="csgA"
                     /locus_tag="ACHUQ7_004390"
     CDS             complement(202..567)
                     /gene="csgA"
                     /locus_tag="ACHUQ7_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFR1137454.1"
                     /translation="MPVHLSNNSGPNSELNIYQYGGGNSALALQTDARNSDLTITQHG
                     GGNGADVGQGSDDSSIDLTQRGFGNSATLDQWNGKNSEMTVKQFGGGNGAAVDQTASN
                     SSVNVTQVGFGNNATAHQY"
BASE COUNT          166 a          146 c          155 g          174 t
ORIGIN      
        1 tcgcgcaggg acaatatttg tactctgcac agacaagatt gagtaagagt aacttcagga
       61 ataatggtgt acatatcccc ttgctgggtc gtattaaagg ttatctggct ggaaagtgcc
      121 gcaaggagta ataacgcatt catattcttc tcccgaaaaa aaacagggct tgcgccctgt
      181 ttctgtaata caaatgatgt attaatactg atgagcggtc gcgttgttac caaagccaac
      241 ctgagtcacg ttgacggagg agttcgatgc agtctggtca actgcagcac cgttgccacc
      301 accgaactgt ttaacagtca tttcagaatt tttgccgttc cactgatcaa gagtggcgct
      361 gttaccgaag ccacgttggg tcagatcgat tgagctgtca tctgagccct gaccaacatc
      421 tgcaccatta ccaccgccat gctgggtaat agtcaagtca gagttacggg catcagtttg
      481 cagagcaagt gcagagttac cgccaccgta ctggtaaatg ttcagctctg aatttgggcc
      541 gctattatta ctgaggtgta ctggcaatag cggacactac catttgttct tttttaagca
      601 gccatctgat gatatttttc cctgaaggct gccggggaga t
//
LOCUS       DAZPCS010000444          629 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZPCS010000444 DAZPCS010000000
VERSION     DAZPCS010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.452"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            61..360
                     /gene="pliG"
                     /locus_tag="ACHUQ7_004391"
                     /pseudo
     CDS             61..360
                     /gene="pliG"
                     /locus_tag="ACHUQ7_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
     gene            complement(468..>629)
                     /locus_tag="ACHUQ7_004392"
     CDS             complement(468..>629)
                     /locus_tag="ACHUQ7_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFR1137455.1"
                     /translation="LQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVDGKRSGKINQTT
                     TKMLFMCRE"
BASE COUNT          199 a          149 c          118 g          163 t
ORIGIN      
        1 agtcacctct gactgagagt ttactcactt agccgcgtgt ccactattgc tgggtaagat
       61 catagcagcg ctcagtactc cggcgaaata aagggatacg attacgatac atataccttc
      121 tatgccaaaa aaggtcagaa agtgcatgtg agtatctcta atgaaggcgc tgatacttat
      181 ctcttcgggc caggaatcga tgattctgtt gatttatcca gatattcccc agaactcgat
      241 agtcacggac aatactcact ccctgcttct ggcaaatatg agctgcgagt gctgcaaact
      301 cgaaacgacg cccgtaaaaa caaaactaaa aaatataacg tcgatattca aattaagtaa
      361 atgtcaaccg acggggacta tccccgtcat agacgttaac gccccactcc tctctgtcgc
      421 tcaagaggtt aatgacgaat agcagagaac ataatgtaaa caaggcatca ctcacgacac
      481 ataaataaca tctttgtggt ggtttgattg atttttccac tccgcttacc atctacacca
      541 aaataacgat catcacgaca taatttttct ttaacatcgc aaaatacacc gttagcgaat
      601 gtaaattcgc tgggatcgta gccttgtaa
//
LOCUS       DAZPCS010000445          624 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZPCS010000445 DAZPCS010000000
VERSION     DAZPCS010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.453"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(29..397)
                     /locus_tag="ACHUQ7_004393"
                     /pseudo
     CDS             complement(29..397)
                     /locus_tag="ACHUQ7_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT          180 a          110 c          142 g          192 t
ORIGIN      
        1 ttgtctggag attcaggggg ccagtctaat cagttgtcat gcactcggaa taaatcatac
       61 cgtgaatagt gctgtttttg attgtcagac tattggcgtt ggtattggta gaaataccgt
      121 cgtccagata gtaggtagaa atcacgccgt taacggtagc gttattaatg accgggtaga
      181 tatcgccgtt gtaatagcta tcagcggcat agttgttcca gcctacataa ccatcatagt
      241 aaacgtcatc agcataggtg gcgtcgttgt agtgatggaa agtattgtaa gtttgaccag
      301 aaatatcgtt ggtcgctgca ttagcctgag aggtgatagc cagagtgcaa gccaatgcta
      361 attgtgatac tacaagtttc tttttccagg agtgcatttg tcatccctcc tcagggacgt
      421 aataagttga tccatcaatt atcaatgcat agaaaacata ttatgtttcg cgctaacgat
      481 tatgctgcgt tacagacgaa tggttcaatt attctttccc ctaagtccga atgtggacat
      541 atataattta tggagaaaaa cacgcatcaa attgataaaa ggacgatatt tcagtatgtt
      601 gcagataatg ttatttttga tgca
//
LOCUS       DAZPCS010000446          622 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZPCS010000446 DAZPCS010000000
VERSION     DAZPCS010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..622
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.454"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            266..341
                     /locus_tag="ACHUQ7_004394"
     tRNA            266..341
                     /locus_tag="ACHUQ7_004394"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:299..301,aa:Ile,seq:cat)
BASE COUNT          177 a          112 c          147 g          186 t
ORIGIN      
        1 cttcagctaa tgtaataaag ttatattatt agatacaaaa tcattacaaa gtatcattga
       61 atcgatgata actatagata gtgctaatta ttaacatttg cttgttgact gcgagtatcg
      121 actttgcgaa tggaggcggt gtcgggtttt atgtcgcgtg taatcacgac ccaaggtgaa
      181 tgggaacgca taaaaatctg ttatttacat cataagatca tcatgtttat tgcgtggttt
      241 ataagtattg ttattgcata cttaaggccc tttagctcag tggttagagc aggcgactca
      301 taatcgcttg gtcgctggtt caagtccagc aagggccacc aaatcatcat tcatgtatca
      361 ggaagagtta agggtacaag gggtactcga tggcggtcta tatttcatga ctaacgccat
      421 ttcaaggaag aagtgcggtg ggtggtttcg aatgagggct ggtgttttgg tacgtggcac
      481 gccactgcaa tgtatttatt catcagtact cattcaaaag tacctggaaa aaatgcccgc
      541 tatgccaagc gggcaaattg atcctaccca cgtaatatgg acacaggcct aagcgaggtt
      601 ctggttttca aattgttccg ga
//
LOCUS       DAZPCS010000447          619 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZPCS010000447 DAZPCS010000000
VERSION     DAZPCS010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.455"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..>619)
                     /gene="ipaH3"
                     /locus_tag="ACHUQ7_004395"
     CDS             complement(<1..>619)
                     /gene="ipaH3"
                     /locus_tag="ACHUQ7_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH3/Hc"
                     /protein_id="HFR1137456.1"
                     /translation="RNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQ
                     NALISLPELPASLEYLDACDNRLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINA
                     DNNQLTMLPELPTSLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHS
                     EETEIFFRCRENRITHIPENILSLDPTCTIILEDNPLSSRIRESLS"
BASE COUNT          164 a          106 c          168 g          181 t
ORIGIN      
        1 gacagagact ccctgatccg tgaggacaga ggattgtctt cgaggatgat agtgcaggtc
       61 ggatcaaggc taagtatatt ttccggaatg tgtgtgatgc gattctcgcg gcaccggaaa
      121 aatatctcgg tttcctctga gtgatgattt cttacaggta cggctggtag gctttccaga
      181 agattagtac ttacatcgag cgcttccagt gattcaggta actcagggag aaatgtcagc
      241 tggttatttc ttactgagag cacttccagc gatgtaggta attcaggaag catggttagc
      301 tgattgttat ctgcattaat atattccagc aatgcaggca attcaggaag catggttagt
      361 tggttgttat ctacatcaag atgtttcaga gatgcgggta attcaggaag tgttgacagg
      421 cgattgtcac aggcgtcaag gtattccagc gatgctggca attctggtaa tgatattagg
      481 gcattctgag taatttccag aacagtgatt tgaggtggta agttgtcagg tagcgaggac
      541 agatttaaac gattcagttg aagctcactg aactgattga tgagacattc tttaagtagg
      601 gagaccgctt cattccgat
//
LOCUS       DAZPCS010000448          612 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZPCS010000448 DAZPCS010000000
VERSION     DAZPCS010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.456"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            163..>612
                     /locus_tag="ACHUQ7_004396"
     CDS             163..>612
                     /locus_tag="ACHUQ7_004396"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1137457.1"
                     /translation="MRAIYLSVQQAWNGKITYSVSGESEFAKKFQGKALPFDVRIISA
                     SQNEDWLVIATKVLPGADLRTYVDFKNSTVHVDSAGLEKVAKCINCNNTLQVNIPHEA
                     GHVLGYLDDDYDSSSPYVGDISGLMNVGMELWERYLKNATITLNIIMP"
BASE COUNT          197 a           79 c          141 g          195 t
ORIGIN      
        1 acaaatattg atggcaatat cattatacat aaggatggta ggaggacgta tttattaaat
       61 caaaatactg gttcgattac ggctgtgata agatggaagt atgagtagta taaggataat
      121 accgttacag atgactggac tatatgggaa aaagctattt ttatgcgagc gatatattta
      181 tctgttcagc aggcatggaa tgggaaaata acttattctg tttccggtga atctgagttc
      241 gctaaaaaat ttcagggtaa agctttgcct tttgatgtaa gaataatttc tgcatctcag
      301 aatgaagact ggttggttat tgcaacaaag gttttgcctg gtgctgattt aagaacatat
      361 gttgatttta aaaacagtac ggttcatgtt gattcagcgg gcctggaaaa agtagcgaaa
      421 tgtattaact gtaataatac gttgcaagta aatatacctc atgaagccgg acatgtactt
      481 ggttacttag atgatgatta tgattcttcg tctccgtatg ttggagatat tagtgggttg
      541 atgaatgtgg gaatggagct ttgggaacga taccttaaaa acgcaactat aacgttgaat
      601 atcataatgc ct
//
LOCUS       DAZPCS010000449          596 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.457, whole genome shotgun sequence.
ACCESSION   DAZPCS010000449 DAZPCS010000000
VERSION     DAZPCS010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..596
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.457"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            1..199
                     /locus_tag="ACHUQ7_004397"
                     /pseudo
     CDS             1..199
                     /locus_tag="ACHUQ7_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611347.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          177 a          134 c          117 g          168 t
ORIGIN      
        1 cccacctgcc gggctaatcc accactccgc tcgaggttca cagtactgcg catacgatta
       61 ccgggtcata caggagcagt ctggtctgaa aacatcaatg tcgcgtaaag gtaactgtta
      121 cgacaacgct ccgatggaaa tcttctgggg aacgctgaaa aatgaaagcc tgagccacta
      181 tcgttttaag agccgggata tttcgtcagc atacgggaaa actgattgag gtcggcctca
      241 gttttgaggc ctttagccag ttcagccgca agggctttaa gcttcttttc gtccataatt
      301 tacctgtctc cgttgttgga gtaaacatat caaaaacagg caattacaca atttaaatta
      361 cagtctcaaa acaaacccaa cggtcaatat agtttaccag caagcttact agcaaagtgc
      421 taatcatttg gtatctttcg tgctattctc cttttacgta agtacttaag gggaacgtat
      481 ttactacttt taaagaaagc tccgttacta tctgaaatgc aatgagataa taaacgatta
      541 ctctcggtta gtatctcatg atcatttaca gactttgcat aatcgcgaat actata
//
LOCUS       DAZPCS010000450          585 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZPCS010000450 DAZPCS010000000
VERSION     DAZPCS010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.458"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..>585)
                     /gene="ipaH9.8"
                     /locus_tag="ACHUQ7_004398"
     CDS             complement(<1..>585)
                     /gene="ipaH9.8"
                     /locus_tag="ACHUQ7_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HFR1137458.1"
                     /translation="RDEAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSY
                     NQLTNLPELPVTLKKLYSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNI
                     SYNEIVSLPSLPQALKNLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILN
                     LRNECSIHISDNPLSSHALQALQRLTSSPDYHGP"
BASE COUNT          161 a           97 c          151 g          176 t
ORIGIN      
        1 gtgggccgtg gtagtccggc gaagaggtta atctttgcag ggcttgcaga gcatgggatg
       61 ataatgggtt atcactaata tgtattgaac attcattcct cagattaaga atgctttccg
      121 ggatatgact tatctgattt ctatcaaaaa aatactctct gacaacggga ttatttccct
      181 cagaaaatgc tggtagctca gtgaggaaat tacgggtcgc tctgagattt ttaagagcct
      241 gtgggagcga tggtaaggag actatttcgt tatagctgat attcatagtc aataacgaat
      301 cgggtaaagc tggcaggttt tccagctcat tgtgttgtac ctgaagtgac tccagcgcag
      361 gaggtagcac gggcaattct gataatttat tgctggcgga atataatttt tttagcgtaa
      421 caggcagttc aggtaggtta gttaattgat tatatgatac attgagcagc gttatctgag
      481 ctggtaagtt gtcaggtagc gaggacagat ttaaacggtc cagtcgaagt tcatcggaat
      541 tattgataag acattcttta agtcgggaga cagcctcatc acgct
//
LOCUS       DAZPCS010000451          580 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZPCS010000451 DAZPCS010000000
VERSION     DAZPCS010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.459"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..318
                     /locus_tag="ACHUQ7_004399"
     CDS             <1..318
                     /locus_tag="ACHUQ7_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1137459.1"
                     /translation="FSQRNPDNSIVFTVNGQVIPLNYGNFDARYKYRTEGVQDVRYGH
                     EMYYSPGSNTVSWRFCAPSGHGLSGMAISDTGRNSADNVDGVYYRPLQKLINGTWYNV
                     ASI"
     gene            321..>580
                     /locus_tag="ACHUQ7_004400"
     CDS             321..>580
                     /locus_tag="ACHUQ7_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR1137460.1"
                     /translation="MLHLKNITAGNPKTAEQYQMTKRYSVTWLFSEDGKNWYEELKNF
                     ASDTIKIAYTGDGRVVWVGKDVTGIEPRNASVIEVPDITANRR"
BASE COUNT          178 a          122 c          149 g          131 t
ORIGIN      
        1 ttcagccaga gaaacccgga taacagcata gtgttcactg ttaacggaca ggtaattccg
       61 ttaaattacg gaaacttcga tgcccgctat aaatatcgaa cagagggggt acaggatgta
      121 cggtatggcc atgaaatgta ttacagcccc ggcagtaaca ccgtttcgtg gagattttgc
      181 gcaccttcgg gacacgggct gtcagggatg gcgatatcgg ataccggccg taactcagcg
      241 gataacgttg acggtgtgta ttaccgacca ctgcaaaaac tgattaatgg cacctggtat
      301 aacgtagcga gtatttaaca atgttgcatt taaaaaatat tactgcgggt aatccgaaaa
      361 cagcagaaca atatcagatg acaaaacgat attcggtcac ctggcttttt tcagaagacg
      421 gaaaaaactg gtatgaagag ctgaagaatt tcgccagcga cacaataaaa atagcttaca
      481 ccggagatgg tcgcgtggtg tgggtcggta aggatgtgac aggcatcgag ccacgcaatg
      541 ccagcgttat cgaagttccg gatattaccg ctaaccgacg
//
LOCUS       DAZPCS010000452          567 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZPCS010000452 DAZPCS010000000
VERSION     DAZPCS010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.460"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(19..294)
                     /locus_tag="ACHUQ7_004401"
     CDS             complement(19..294)
                     /locus_tag="ACHUQ7_004401"
                     /inference="COORDINATES: protein motif:HMM:NF018642.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
                     /protein_id="HFR1137461.1"
                     /translation="MRDVQWFCPLTPVHTGTEIKPVEMPVTTITPVSDAGGLQDFIYW
                     RPDADRAGMEPVYVILNDPLDSGRFTRKQLNKNILNMQVILVFSIQE"
BASE COUNT          151 a          134 c          122 g          160 t
ORIGIN      
        1 tatagtagtt actactattt attcttgtat cgagaatacc aaaattactt gcatgtttaa
       61 gatattttta tttagttgtt tacgcgtaaa tcgcccggaa tccagaggat cattaagtat
      121 cacataaaca ggttccatac cagccctgtc cgcatcagga cgccagtaaa taaaatcttg
      181 tagtccaccg gcatcagaga caggagtaat cgtcgttacc ggcatttcga ctggttttat
      241 ttccgtacct gtatgtactg gtgtgagcgg acaaaaccac tgtacgtctc tcatacacgc
      301 tcgtaagcgg ccctcacggc ccgctaacgc ggagatacgc cccgacttcg ggtaaaaccc
      361 tcgtcgggac cactccgacc gcgcacaaaa gcattttcat cgccgatagc gggtatggct
      421 tggcagggcc gggatgggta taggtaattt ggctatcgta aaaaaggtgg ttaaaaatta
      481 caacctgcaa tgtaaattgg ctagttaatt tttggctatt taaattgact tcttaacggc
      541 gtttactgtt ccattgctcc ccaaccc
//
LOCUS       DAZPCS010000453          559 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZPCS010000453 DAZPCS010000000
VERSION     DAZPCS010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.461"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..>559
                     /locus_tag="ACHUQ7_004402"
     CDS             <1..>559
                     /locus_tag="ACHUQ7_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HFR1137462.1"
                     /translation="APTLRIRDPHKDEKMAAIHKALDECSAEHPVFYEDEVDIHLHPK
                     IGADWQLRGQQKRVVTPGQNEKYYLAGALHSGTGKVSYVGGNSKSSALFISLLKRLKA
                     TYRRAKTITLIVDNYIIHKSRETQSWLKENPKFRGIYQPVYSPWVNHVERLWQALHDT
                     ITRNHQCRSMWQLLKKVRHFMETVSP"
BASE COUNT          165 a          138 c          148 g          108 t
ORIGIN      
        1 tgcgccaact ctgcgtatcc gtgacccgca taaagatgaa aagatggcag caatccataa
       61 agcactggac gaatgcagcg cagagcatcc ggtcttttat gaagatgaag tggatatcca
      121 tcttcatccc aaaatcggtg cggactggca actgcgcgga cagcaaaaac gggtggtcac
      181 gccgggacag aatgaaaaat attatctggc cggagcgctg cacagcggga caggtaaagt
      241 cagctatgtg ggcggcaaca gcaaaagttc ggcgctgttc atcagcctgc tgaagcggct
      301 taaagcgaca taccgtcgtg cgaaaaccat cacgctgatc gtggacaact acattatcca
      361 caaaagccgg gaaacacaga gctggctgaa ggagaacccg aagttcaggg gcatttatca
      421 gccggtttac tcgccatggg tgaatcatgt tgaacggcta tggcaggcac ttcacgacac
      481 aataacgcgt aatcatcagt gccgctcaat gtggcaactg ttgaaaaaag ttcgccattt
      541 tatggaaacc gtcagccca
//
LOCUS       DAZPCS010000454          554 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZPCS010000454 DAZPCS010000000
VERSION     DAZPCS010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.462"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..>554)
                     /locus_tag="ACHUQ7_004403"
     CDS             complement(<1..>554)
                     /locus_tag="ACHUQ7_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MolR family transcriptional regulator"
                     /protein_id="HFR1137463.1"
                     /translation="VEQAKDNSLQPSQTVTDSLKVADLSTIIQEQPSFVAETRAADKN
                     TDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVSQRDNKT
                     ATFDFSTCSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVREDVMDQ
                     IVQEGSLEYATDVIIHLQQIDIKW"
BASE COUNT          134 a          119 c          135 g          166 t
ORIGIN      
        1 ccatttaata tcaatctgtt gaaggtgtat tattacatca gtagcatatt ccagactgcc
       61 ttcctggacg atctgatcca taacatcctc acgcacttta tatcttgtgc atttcatttc
      121 aagtgccgtg agtacagcca ttactggtga tggtagctgt gttgttttca ggccgtcgat
      181 ctgactgatc gcttgggcga cggtgttttg ccattctaaa gaacaggtgc tgaagtcaaa
      241 tgtggctgtt ttattatccc gttgcgacac gctacaggct aagcgacgta attgggtcaa
      301 tttgtctgct tgctgaacag gaactccggg aaagcggcga tgacttaacg tggtgtgtac
      361 aacttcgggc ggaaaaacga cagcaatatc tttcgccagc caaggtaaaa cagcatctgt
      421 atttttgtca gccgcacggg tttctgctac gaaagagggt tgttcctgaa taatggtgga
      481 taagtccgct accttgagag agtccgttac cgtttgggaa ggttgcaaag aattatcttt
      541 cgcttgctcc acat
//
LOCUS       DAZPCS010000455          550 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZPCS010000455 DAZPCS010000000
VERSION     DAZPCS010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.463"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..>550)
                     /locus_tag="ACHUQ7_004404"
     CDS             complement(<1..>550)
                     /locus_tag="ACHUQ7_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR1137464.1"
                     /translation="AFIHFYDYSRRYAISENIDKDAVTNNIALMSTFYDSDNTSGEVL
                     NNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTRFSAFQDTVFNLFPEMH
                     SSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMVREDASNLVNLKTLLND
                     GCFIKYSHFNDVALIKQNISNLY"
BASE COUNT          183 a          108 c           82 g          177 t
ORIGIN      
        1 atagagatta cttatgtttt gctttattaa tgccacgtca ttaaaatgac tatatttaat
       61 gaaacaacca tcattgagta gtgttttaag attgactaaa ttacttgcat cttcacgtac
      121 catcgtggca actggaacag gcagagacat gaaaacttct tcattcacca tcaagtgaat
      181 catgcattgc gttccctttg tcatatccac acttacgcta caaatatcgt ggtaatgcga
      241 agagtgcatt tcaggaaaca gattaaacac ggtatcttga aaggcactaa atcgtgttaa
      301 taaatcctga taatgaacag gttttgtttc aaaattatca tcagcaaatc caagcactat
      361 tgcatgctct acatctaacg cagtttcaaa tgactcttct tctgtaaaat cattatttaa
      421 aacctcacct gacgtattat cactgtcata aaaagtgctc atcaatgcga tattgtttgt
      481 tacagcgtct ttatcgatat tctcactaat agcatatcgc ctgctataat cataaaaatg
      541 aataaatgcg
//
LOCUS       DAZPCS010000456          546 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.464, whole genome shotgun sequence.
ACCESSION   DAZPCS010000456 DAZPCS010000000
VERSION     DAZPCS010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.464"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..>546
                     /gene="ospB"
                     /locus_tag="ACHUQ7_004405"
     CDS             <1..>546
                     /gene="ospB"
                     /locus_tag="ACHUQ7_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HFR1137465.1"
                     /translation="VINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSP
                     GSHQLGLGSELIDVQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSC
                     ILNSLPFFKEKESLLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTS
                     IPADPEHTVKRSSQKKTFIIN"
BASE COUNT          182 a           93 c           97 g          174 t
ORIGIN      
        1 tagtaataaa taatgatgat gacgcattga atgctgctgc ttatttttct gtaaaaaaag
       61 cgaaaataaa aacagtaaac gatactgatt ttaaagagta taataaggtt tatattcttg
      121 ggcacggtag tcctggttct catcaattag gccttggttc ggaacttatt gatgtacaaa
      181 caatcatttc aagaatgaaa gactgtggta ttctaaatgt gaaagatatc cgttttactt
      241 catgcggctc cgctgataaa gtggctccta aaaattttaa caatgcccct gctgaaagtc
      301 tttcttgtat ccttaactct ctgccttttt ttaaggaaaa agaatctttg ctagagcaga
      361 taaaaaaaca ccttgaaaac gatgagtcat tgagtgatgg tctaaaaata tccggctatc
      421 atggatatgg agttcactat ggtcaagagc tttttcccta ctcacattat cgttcaactt
      481 caattcctgc tgatccggag catacagtaa aaagaagctc tcagaaaaag acttttatta
      541 ttaata
//
LOCUS       DAZPCS010000457          537 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.465, whole genome shotgun sequence.
ACCESSION   DAZPCS010000457 DAZPCS010000000
VERSION     DAZPCS010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.465"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            1..487
                     /locus_tag="ACHUQ7_004406"
                     /pseudo
     CDS             1..487
                     /locus_tag="ACHUQ7_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT          124 a          118 c          136 g          159 t
ORIGIN      
        1 actgggagca ttgtgggtgc gcaatggcat ggaggagtcc gcggaatttg aacaacagca
       61 acataatcaa gcggccgcga aaaaacgcat cccggttatc gaagctctgt tacgacatcc
      121 cggtgctttc ctgaagatta ttgcactacg actgtgcgag ttgctgacga tgtacattgt
      181 tactgccttt gcacttaatt attcaaccca gaatatgggg ttaccgcgcg aacttttcct
      241 taatattggt ttgctggtag gtggattaag ctgcctgaca attccctgtt ttgcctggct
      301 tgccgatcgt tttggtcggc gcagggttta tatcacaggc gcgttgatcg gaacgttgag
      361 cgcatttcct ttctttatgg cgcttgaagc acaatctatt ttctggatag ttttcttctc
      421 cataatgctg gcaaacattg cgcatgacat ggtggtgtgt gtgcaacaac cgatgtttac
      481 cgaaatgtga ggtagcctga gtttaacgga cactccttcc tgaaatagaa tggcatc
//
LOCUS       DAZPCS010000458          529 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZPCS010000458 DAZPCS010000000
VERSION     DAZPCS010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.467"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            381..>529
                     /locus_tag="ACHUQ7_004407"
     CDS             381..>529
                     /locus_tag="ACHUQ7_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFR1137466.1"
                     /translation="MSQQPQQPQQEISIINLDQLVSMTSVEIAELTGKEHRNVLRDIR
                     KMLEEL"
BASE COUNT          129 a          140 c          115 g          145 t
ORIGIN      
        1 tagggtctct ctataggtct tctctatagg tcttctctat ggtctctcta taggtctctc
       61 tagggtctcc tctaggcatt acccccagaa gctaactcta tggccctcta tggtcctcta
      121 tggccctgta cgtcattctt taggatgccc tgtagtttcg tatagcctga tgcctagaga
      181 gaccctagag agaccctaga gtggctccta gagagaccct cccagtagct ccgcacagct
      241 acatcgtcat catcatagta ttcaatctca aagtcattct gtttgcatcc tgttgtcctg
      301 ctggtctccc ttcgcttggg gactttgagt aggtcagtgg gggtgcgact tcagggtgca
      361 aacaaaaagg aaatcaagcg atgtctcaac aaccacaaca accacaacaa gaaatcagca
      421 tcattaacct cgaccagctt gtgtccatga cctcagttga aattgctgag ctgacaggca
      481 aggagcaccg aaatgtcctt cgtgatattc gtaagatgct cgaagaact
//
LOCUS       DAZPCS010000459          511 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.468, whole genome shotgun sequence.
ACCESSION   DAZPCS010000459 DAZPCS010000000
VERSION     DAZPCS010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.468"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            1..511
                     /locus_tag="ACHUQ7_004408"
                     /pseudo
     CDS             1..511
                     /locus_tag="ACHUQ7_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element ISSfl7 family transposase"
BASE COUNT          127 a          128 c          141 g          115 t
ORIGIN      
        1 acaccacggg ccggacgccc atgggctgag caccgtaaaa tcatcaatgg catgttctgg
       61 gtgttatgtt ccagtgcacc atggcgcgat ttacccgaac gatatggggc atggaaaact
      121 gtttataacc gctttaaccg atggtcaaag tcaggcgtga ttaatattat tttcaacagg
      181 ttgctttcgc tacttgatgc aaacggcttt attgactggt ctgccaccgc gctggatggc
      241 agtaatatcc gggcgctgaa atgtgccgcc ggtgctcaaa aaaacatccc gatatcgacg
      301 gagataatgg gccgggtcgc tctcgcggcg gttttggcac caaaatccat ctggcaacag
      361 acggaagtgg cctcccgtta aatatcgtgc taagccccgg acaagctcat gaaagccagt
      421 tcgcgcaacg acttctggac gggattggcg ttcagcgtca gaacggcagc atgaaacgcc
      481 gtggacatgc ggtactggct gataaagcgt a
//
LOCUS       DAZPCS010000460          492 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.470, whole genome shotgun sequence.
ACCESSION   DAZPCS010000460 DAZPCS010000000
VERSION     DAZPCS010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.470"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(199..>492)
                     /locus_tag="ACHUQ7_004409"
     CDS             complement(199..>492)
                     /locus_tag="ACHUQ7_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005137803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1137467.1"
                     /translation="IHHSGVVNISSDNLQNILSKPREHTLNCLGWRFFVQRSVCVSWK
                     SDFAWTGIMLLFRGEELAFSSAFSPVTIRKIMKCPPPGRRTVEKRTRQQKHWG"
BASE COUNT          149 a          115 c           97 g          131 t
ORIGIN      
        1 ccccgacaga cctgtgctat cggaaagaaa aaaggccatc agcaaaactc tgatgacctg
       61 aatcaccttt accaatattg tatgaaaaaa cacgcacgac taattgacaa taattttcat
      121 ttccattaaa aaagatcgcg tagcattctt aattcatgag gaactttacc cgccagcaat
      181 ctgagtagcg aaagctgttc atccccaatg tttttgctgg cgggtccttt tttctacggt
      241 cctcctcccc ggaggggggc atttcattat ttttctaatc gttactggtg agaaggcgga
      301 agaaaaagcc agttcttcgc ccctaaataa cagcatgatt ccagtccatg caaaatctga
      361 tttccagctc acacataccg agcgctgtac aaaaaaccgc cagccaaggc agttaagagt
      421 gtgttcccgg ggtttgctta gaatattttg taagttgtcc gaagagatat ttacaacacc
      481 agaatgatga at
//
LOCUS       DAZPCS010000461          484 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.471, whole genome shotgun sequence.
ACCESSION   DAZPCS010000461 DAZPCS010000000
VERSION     DAZPCS010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.471"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            1..484
                     /locus_tag="ACHUQ7_004410"
                     /pseudo
     CDS             1..484
                     /locus_tag="ACHUQ7_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          177 a           81 c           96 g          130 t
ORIGIN      
        1 gtcgagtaat aatgaatatg acctgtttaa aaatagagaa acctcactgt ggaaacgcaa
       61 agtaccagaa agcgcagcgc tggcaatgac gggaacagta tataaattat ttggtaatcg
      121 cttactcaac gcccgcaatc agttagataa aaatctcctc gaaatggcta tgcaaatggt
      181 ttatggcaaa gatatggtga caaagtccgg ggatatattg attgatatac aattaaataa
      241 ggatggcagt ctccagagta agcactacac acttgatgta ggtgaagtta taaacagtta
      301 taatattgac cctctttgtg aatgcttcaa gtattaatca aacttactta catgataaaa
      361 atcctggtga cgttattagc ctgtatggca ctttagataa cattcccctt gtagttaagg
      421 aatgcacggg taaaaatcat caaattaatg ttaaagccaa attgccacaa gaacgaagtg
      481 aaaa
//
LOCUS       DAZPCS010000462          483 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.472, whole genome shotgun sequence.
ACCESSION   DAZPCS010000462 DAZPCS010000000
VERSION     DAZPCS010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.472"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..139
                     /locus_tag="ACHUQ7_004411"
     CDS             <1..139
                     /locus_tag="ACHUQ7_004411"
                     /inference="COORDINATES: protein motif:HMM:NF025073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1137468.1"
                     /translation="AGLKPDTMRNVFRCHIPRYEAIIARYLDMEPAQIWPSRYMEKTS
                     C"
     gene            133..483
                     /locus_tag="ACHUQ7_004412"
                     /pseudo
     CDS             133..483
                     /locus_tag="ACHUQ7_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955480.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
BASE COUNT          140 a          108 c          119 g          116 t
ORIGIN      
        1 ggcaggctta aagcctgaca ctatgcgtaa cgtatttcgc tgccatatac cacgatacga
       61 agcaatcatt gctagatatt tggatatgga accagcccag atttggcctt ctcggtatat
      121 ggagaaaaca tcatgttaac ccattgggta acagcgcaag aatgcgtagg aatgtcatgc
      181 tttcctaaac atgaggtgag tattcgccgc aatcttgaaa agcttgcagc caatcatcca
      241 gaactacgtc gcaagcgtac gggttcaaag gctttggaat ttcatatatc agttctacca
      301 gcggcagcac gtgctgagtt gttattaaaa tctgagcaaa tagaaacaag ctaggggtat
      361 tttgaaatcg cccgccccac gctggaagcg cacgattacg atcgtgaggc actgtggagc
      421 aaatgggata aagccagcga ttcccagcgc agacttgctg aaaaatggtt gcctgcggtt
      481 cag
//
LOCUS       DAZPCS010000463          480 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.473, whole genome shotgun sequence.
ACCESSION   DAZPCS010000463 DAZPCS010000000
VERSION     DAZPCS010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.473"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..85
                     /gene="yjbL"
                     /locus_tag="ACHUQ7_004413"
     CDS             <1..85
                     /gene="yjbL"
                     /locus_tag="ACHUQ7_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YjbL"
                     /protein_id="HFR1137469.1"
                     /translation="DIIIAKIDLFIQRITHNLWHARKKMCF"
     gene            109..>480
                     /locus_tag="ACHUQ7_004414"
     CDS             109..>480
                     /locus_tag="ACHUQ7_004414"
                     /inference="COORDINATES: protein motif:HMM:NF022352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HFR1137470.1"
                     /translation="MWVNKYTDDCTDEDLNDRDFIASVVDRAIFHFAINSICNPGDNK
                     DAMPIEQCTFDVETKNGLPSTVQLFYEESKDNEPLANIHFQAIGSGFLTFVNACQEHD
                     DNSLKLFASLLISLSYSSAYAD"
BASE COUNT          151 a           76 c           89 g          164 t
ORIGIN      
        1 ggatataatt attgcaaaaa tagatttgtt tattcaacga atcacgcata atttatggca
       61 tgcacgtaaa aaaatgtgtt tttaattgag cacataaatg atctcaaaat gtgggtcaat
      121 aagtatactg acgattgcac tgatgaggat ttaaacgatc gtgactttat tgcatcagtt
      181 gttgaccggg ctatttttca tttcgcgatt aatagtatat gtaatcctgg ggataataaa
      241 gatgcaatgc ccattgaaca atgtactttt gatgtagaaa ctaagaatgg ccttccctcc
      301 acggttcagc tattttatga ggaatctaag gataatgaac ctttagcgaa tatacatttt
      361 caagcaatag gttctggttt tttaacgttt gttaatgcct gccaggaaca tgatgacaac
      421 agcttaaaat tatttgcttc gctgttaatt tcactttcat attctagtgc ctacgcagat
//
LOCUS       DAZPCS010000464          480 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZPCS010000464 DAZPCS010000000
VERSION     DAZPCS010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.474"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..212)
                     /locus_tag="ACHUQ7_004415"
     CDS             complement(<1..212)
                     /locus_tag="ACHUQ7_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1137471.1"
                     /translation="MEKIKKLFSSKYAVIRRDDLSVIVEMDYFPETPKSIMYRNGRKA
                     IFLPMRVSDIMGNDKLLDELRVRASC"
BASE COUNT          135 a           98 c           89 g          158 t
ORIGIN      
        1 taacaggatg ctctgactcg caattcatcc agcagtttat catttcccat aatgtcactt
       61 accctcatcg gtaaaaaaat tgcctttcga ccattacgat acattattga ttttggggtt
      121 tcagggaagt aatccatttc gactataact gacaggtcat cacgacgtat gactgcgtat
      181 ttgctactaa atagtttctt tattttttcc acgatgcccc caggtttata agtacaaacg
      241 gttatatcca catagagaca aaaatattaa tctgataaat atttatttca cgccgtatat
      301 ttgattgttt aatgtgcaag tacaatgact tttatttttt gttgtgtata taatcaaata
      361 tatggttatt tttcaccctg cgtattcagc acgcaacaaa aaacccgccg aagcgggttt
      421 agtgcgggtg cgttgaggat gcctgacaca tgagaggtgg cgagggattt ctcccccgcc
//
LOCUS       DAZPCS010000465          475 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.475, whole genome shotgun sequence.
ACCESSION   DAZPCS010000465 DAZPCS010000000
VERSION     DAZPCS010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.475"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..>475)
                     /locus_tag="ACHUQ7_004416"
     CDS             complement(<1..>475)
                     /locus_tag="ACHUQ7_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1137472.1"
                     /translation="VGQFNKIAIFLIFTVCWVLSIIKRQQFRWLAINNIEFSTLFVIL
                     FLVLIFVVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYLRFPLFPQNADLLM
                     ALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYPGIIATILLFTINPLKD"
BASE COUNT          191 a           94 c           74 g          116 t
ORIGIN      
        1 atctttaagt ggattgatag taaatagtag gattgtagca ataatgcctg gataataagt
       61 tttcgtaatt tcccatgaac agccaacaag tccacaggct attacaaaaa aacagatatt
      121 tgctaaaaac tgagctaacc ttacatctcc taactgtaaa cctaaagcca ttaaaagatc
      181 tgcattttgc gggaacagag gaaaacgcaa atattgttca acaacaattg catggtgctc
      241 aactaatgaa cgagccaaag gtaagtgata catagtatca tcccaatcac cgggagctcg
      301 cagggacgat aacaatgtaa ccacaaaaat caaaacaaga aataaaataa caaacagcgt
      361 actgaactca atattattta ttgccagcca tctaaattgc tgacgtttaa taatgcttaa
      421 aacccaacac accgtaaaaa ttaaaaagat agcaatctta ttaaattgcc cgacc
//
LOCUS       DAZPCS010000466          475 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.476, whole genome shotgun sequence.
ACCESSION   DAZPCS010000466 DAZPCS010000000
VERSION     DAZPCS010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.476"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
BASE COUNT          136 a          103 c          110 g          126 t
ORIGIN      
        1 gattgatttt ttattaaaaa atggagcaat attaggcaaa cgatttgaga tatgacctaa
       61 aagctcggcc aaataataat gtcgtcgatt atcgaagtca tcatgagtga ttctgtcccc
      121 acagtgcagc atcagcatga cagtgaattg tctggcgtgg tccatttaat gagatggaga
      181 tggtccctct ttcctaaata gctgtgccta ataccgctac acttttgcca gcccatgttt
      241 gcctccgggg aatgggctga cggttccttc aaaatccatt gcacgcagtt atgtcggagc
      301 gggaacttga ctcctgtctt tacgggagtg aggtagcctg agtttaacgg acactccttc
      361 ctgaaataga atggcatcag aaggagccaa taatgagcag aaaaacccaa cgttactcta
      421 cggagtttaa agccgaagcc gtcaaaacgg ttcctgaaaa tcagctttcg atcag
//
LOCUS       DAZPCS010000467          474 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.477, whole genome shotgun sequence.
ACCESSION   DAZPCS010000467 DAZPCS010000000
VERSION     DAZPCS010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.477"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            57..443
                     /locus_tag="ACHUQ7_004417"
                     /pseudo
     CDS             57..443
                     /locus_tag="ACHUQ7_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014656611.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          152 a           71 c           97 g          154 t
ORIGIN      
        1 tttatggtat catgatgatg tggtcgtgac aatcttaaga acatttaggt tattttatgt
       61 atattgaaca gcattctcgc tatcaaaata aagctaataa catccaatta agatatgatg
      121 ataagcagtt tcatacaacg gttatcaaag atgttctatt atggattgaa cataatttag
      181 atcagtcttt actgcttgat gatgtggcga ataaagcggg ttataccaag tggtattttc
      241 agcggctgtt caaaaaagta acaggggtca cactggctag ctatattcgt gctcgtcgtt
      301 tgacgaaagc ggctgttgag ttgaggttga cgaaaaaaac tatccttgag atcgcattaa
      361 aatatcaatt tgattcccaa caatctttta cacgtcgatt taagtacatt tttaaggtta
      421 caccaagtta ttatcggcgt aataaattat gggaattgga ggcaatgcac tgag
//
LOCUS       DAZPCS010000468          473 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.478, whole genome shotgun sequence.
ACCESSION   DAZPCS010000468 DAZPCS010000000
VERSION     DAZPCS010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.478"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            1..473
                     /locus_tag="ACHUQ7_004418"
                     /pseudo
     CDS             1..473
                     /locus_tag="ACHUQ7_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          104 a          117 c          147 g          105 t
ORIGIN      
        1 ccgttgaata tggacgggca aaaaagtgga tagcgcacgc gcccttattg cccggggatg
       61 gggagtaagc ttagtcagcc gttgtctccg ggtgtcgcgt gcgcagttgc acgtcattct
      121 cagacgaacc gatgactgga tggatggccg ccgcagtcgt cacactgatg atacggatgt
      181 gcttctccgt atacaccatg ttatcggaga gctgcccacg tatggttatc gtcgggtatg
      241 ggcgctgctt cgcagacagg cagaacttga tggtatgcct gcgatcaatg ccaaacgtgt
      301 ttaccggctc atgcgccaga atgcgctgtt gcttgagcga aaacctgctg taccgccatc
      361 gaaacgggca catacaggca gagtggccgt gaaagaaagc aatcagcgat ggtgctctga
      421 cgggttcgag ttctgctgtg ataacggaga gagactgcgt gtcacgttcg cgc
//
LOCUS       DAZPCS010000469          472 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.479, whole genome shotgun sequence.
ACCESSION   DAZPCS010000469 DAZPCS010000000
VERSION     DAZPCS010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.479"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            3..>472
                     /locus_tag="ACHUQ7_004419"
     CDS             3..>472
                     /locus_tag="ACHUQ7_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HFR1137473.1"
                     /translation="MDAGGLRDIEVEAVTKMLACGTRILGVKEYICDKPECPHVRYVT
                     NSCGSRACPSCGKKATDLWIATQLNRLPDCDWVHLVFTLPDTQWPVFESNRWLLNDVC
                     RLAVENLLYAARKRGQEPGIFCAIHTYGRRLNWHPHVHVSVTCGGLNKHGQWKKL"
BASE COUNT          101 a          130 c          144 g           97 t
ORIGIN      
        1 tcctggatgc gggcggtctg cgcgatatcg aggttgaggc tgtcacaaaa atgctggcct
       61 gcggcacgcg gatactgggc gtgaaggaat acatctgcga taaacctgag tgcccccacg
      121 tcagatacgt cactaactca tgcggcagcc gtgcctgccc gtcctgcgga aaaaaggcca
      181 cagacctgtg gatagcgaca cagctgaatc gtcttcctga ctgcgactgg gtacacctgg
      241 tcttcaccct gccggacacg cagtggccgg tgttcgaaag caaccggtgg ctgctgaatg
      301 acgtgtgccg tctggcggtg gagaatctgc tgtatgccgc ccggaaacgg gggcaggaac
      361 ccggtatctt ctgcgccatc cacacgtatg gccgtcgtct caactggcat ccgcatgtac
      421 atgtgtctgt aacctgtgga ggtctgaata agcatggtca gtggaaaaag ct
//
LOCUS       DAZPCS010000470          471 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.480, whole genome shotgun sequence.
ACCESSION   DAZPCS010000470 DAZPCS010000000
VERSION     DAZPCS010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.480"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..361
                     /locus_tag="ACHUQ7_004420"
     CDS             <1..361
                     /locus_tag="ACHUQ7_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR1137474.1"
                     /translation="DHKTGETATETLTQRELVARLKQHIPEKFFKMVRYFGFLANRVC
                     GEKLPQVYRALGMDKPEPVAKVCYAQMVKQFLSRDPFECVLCGGRMVYRRAIAGLNVE
                     GLKKNARDISLLRYMPA"
BASE COUNT          128 a           99 c          161 g           83 t
ORIGIN      
        1 ggaccacaaa acgggagaaa cggcgacgga aacgctgaca cagcgtgagc tggtcgcgag
       61 gctgaaacag cacatcccgg agaagttttt taagatggtg aggtacttcg ggttccttgc
      121 caaccgggtg tgtggagaga agctgccgca ggtgtaccgt gctctgggga tggataaacc
      181 ggaaccagtg gcgaaagtgt gctatgcaca aatggtgaaa cagttcctga gtcgtgaccc
      241 gttcgaatgc gtgctgtgtg gcggccggat ggtataccgc cgggccatcg cgggactgaa
      301 tgtggagggg ctgaagaaaa acgcgcggga tatcagtctg ctgaggtata tgccggcctg
      361 acacaggcta agtgtgcccg ggaatcggtg caagtataaa aaacagcctg ttgcggtata
      421 agaatcgacc atgcagagag agaaaacacc ggagtggagg gaaaagcaga a
//
LOCUS       DAZPCS010000471          469 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.481, whole genome shotgun sequence.
ACCESSION   DAZPCS010000471 DAZPCS010000000
VERSION     DAZPCS010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.481"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..124)
                     /locus_tag="ACHUQ7_004421"
     CDS             complement(<1..124)
                     /locus_tag="ACHUQ7_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1137475.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLII"
     gene            142..333
                     /locus_tag="ACHUQ7_004422"
     CDS             142..333
                     /locus_tag="ACHUQ7_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFR1137476.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
BASE COUNT          123 a          115 c           83 g          148 t
ORIGIN      
        1 aaataattaa attaacttcc ataacattaa aatatgtatc cactgacgct tttttacata
       61 acgaagaatt gaccattttg tcctgttgtg ccttaatgta agtaccgtcc acagcgtggg
      121 acatacttca aggaaccttt tgtgagtcag gcaactagta tgcgaaaacg acaccgattt
      181 aacagtcgca tgacccgtat cgtactgctc atcagcttta tcttcttctt tggccgtttt
      241 atctactcgt ccgtcggtgc ctggcagcac catcagagca aaaaagaagc tcagcaatcc
      301 acactctccg tcgaatcacc ggtacaacgt tagcggttac cttctccact ttcacagaac
      361 ataacggcac ttcgctgtcg gatgcttttg ctctttggga ttatcaaagc ggcagatatt
      421 ctttcatctt aaattttacg tctttgtcct gactgatgtt tatcctgtt
//
LOCUS       DAZPCS010000472          465 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.482, whole genome shotgun sequence.
ACCESSION   DAZPCS010000472 DAZPCS010000000
VERSION     DAZPCS010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.482"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..>465
                     /locus_tag="ACHUQ7_004423"
     CDS             <1..>465
                     /locus_tag="ACHUQ7_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HFR1137477.1"
                     /translation="SGSTLISRDNKLVENFIDTLENWRDQKQIISVICKDGIKIDDSI
                     ITSFKASKDVGISNGLRIQLTFQEINFKAIVGQTDVSAATGRTSTTNDGGATSKKNTG
                     NTTTSLGSPMLTCKELFSYSASELSDEALKARVTCSKSVSVKNGESTFTASA"
BASE COUNT          147 a           96 c          102 g          120 t
ORIGIN      
        1 aatccggtag tacccttatc tcacgagata ataagctggt ggagaacttc atcgacactc
       61 ttgaaaactg gcgtgaccag aagcagatta tttcagttat ctgtaaagat ggtattaaga
      121 ttgacgactc cattattaca agctttaaag cctctaaaga tgttggaatt tcaaacggac
      181 tgagaatcca gctaactttt caggaaatta acttcaaagc tattgtaggg caaactgatg
      241 tttcagcagc cactggacgg acttctacca cgaatgatgg cggtgctacc agtaaaaaga
      301 acactggcaa cactacgaca agtcttggaa gccctatgct gacctgtaaa gagcttttta
      361 gctattcggc aagtgagtta tcagatgaag cgttaaaagc ccgtgtaacg tgctctaaga
      421 gcgttagcgt taaaaatggt gagagcacct tcacagcctc agcta
//
LOCUS       DAZPCS010000473          463 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.483, whole genome shotgun sequence.
ACCESSION   DAZPCS010000473 DAZPCS010000000
VERSION     DAZPCS010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.483"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            1..463
                     /locus_tag="ACHUQ7_004424"
                     /pseudo
     CDS             1..463
                     /locus_tag="ACHUQ7_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          109 a          133 c          136 g           85 t
ORIGIN      
        1 gaacgtgaac tggagatgtc taccgagatt gcacgtctca aacgccagct ggcagaacgg
       61 gatgaagagc tggctatcct ccaaaaggcc gcgacatact tcgcgaagcg cctgaaatga
      121 agtatgtctt tattgaaaaa catcaggctg agttcagcat caaagcaatg tgccgcgtgc
      181 tccgggtggc ccgcagcggc tggtatacgt ggtgtcagcg gcggacaagg ataagcacgc
      241 gtcagcagtt ccgccaacac tgcgacagcg ttgtcctcgc ggcttttacc cggtcaaaac
      301 agcgttacgg tgccccacgc ctgacggatg aactgcgtgc tcagggttac ccctttaacg
      361 taaaaaccgt ggcggcaagc ctgcgccgtc agggactgag ggcaaaggcc tcccggaagt
      421 tcagcccggt cagctaccgc gcacacggcc tgcctgtgtc aga
//
LOCUS       DAZPCS010000474          459 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.484, whole genome shotgun sequence.
ACCESSION   DAZPCS010000474 DAZPCS010000000
VERSION     DAZPCS010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.484"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..>459)
                     /locus_tag="ACHUQ7_004425"
     CDS             complement(<1..>459)
                     /locus_tag="ACHUQ7_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdaU family protein"
                     /protein_id="HFR1137478.1"
                     /translation="LSNRVASYGAIMAALPYMQLYIADYLADTMHLSAEEHGAYLLLM
                     FNYWQTGKPIPKNRLAKIARLTNERWVDVEPSLREFFCDNGDEWMHLRIEEDLASVRE
                     KLTKKSAAGKASVQARRSRKEADVQTKQERNLTGVQTDVGVVFEHDAGFA"
BASE COUNT          106 a          117 c           90 g          146 t
ORIGIN      
        1 gggcaaatcc agcatcatgt tcgaacacca ctccaacatc tgtttgaaca cctgttaaat
       61 ttctctcttg ttttgtttga acatctgctt cctttctgct tcttctggcc tgaacagatg
      121 cttttcctgc ggctgatttt ttggttaatt tttctctgac agatgccaga tcttcctcaa
      181 tccgaagatg catccattcg tcgccgttat cacaaaaaaa ctcccgcaag gatggttcaa
      241 catcaaccca tcgctcgtta gtcagacggg caatttttgc caacctgttt ttgggtattg
      301 gctttcctgt ttgccagtaa ttgaacatca gcaacaaata cgcgccgtgc tcctctgctg
      361 acaaatgcat ggtgtcagcc aggtaatcag ctatgtacag ttgcatgtat ggtaatgcgg
      421 ccataattgc cccgtatgat gctacccggt tgcttagaa
//
LOCUS       DAZPCS010000475          455 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.485, whole genome shotgun sequence.
ACCESSION   DAZPCS010000475 DAZPCS010000000
VERSION     DAZPCS010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.485"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..>455)
                     /locus_tag="ACHUQ7_004426"
     CDS             complement(<1..>455)
                     /locus_tag="ACHUQ7_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HFR1137479.1"
                     /translation="LEELNAGDNKIKELPFLPENLTHLRVHNNRLHILPLLPPELKLL
                     VVSGNRLDSIPPFPDKLEGLALANNFIEQLPELPFSMNRAVLMNNNLTTLPESVLRLA
                     QNAFVNVAGNPLSGHTMRTLQQITTGPDYSGPRIFFSMGNSATISAPEH"
BASE COUNT          130 a           82 c          102 g          141 t
ORIGIN      
        1 gtgttctgga gcggaaattg tggcagaatt tcccatagag aaaaatattc gaggaccaga
       61 ataatctggt ccggtggtta tttgttgtag tgtacgcatg gtatggccag acaatggatt
      121 acctgcaaca tttacgaagg cattctgagc taatctcagg acactttccg gaagtgttgt
      181 cagattatta ttcattagca cagccctgtt catactaaaa ggtaattccg gtagttgttc
      241 tataaaatta ttagccagag ccagcccttc aagcttatct ggaaaggggg gaatgctgtc
      301 taatctgttt ccagaaacta ccagtaattt tagttccggt ggcaatagtg gcagaatatg
      361 caatcggtta ttatgaaccc gtaaatgagt tagattttca ggaagaaatg gtaattcttt
      421 tatcttgtta tcacccgcat taagctcttc taacg
//
LOCUS       DAZPCS010000476          455 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.486, whole genome shotgun sequence.
ACCESSION   DAZPCS010000476 DAZPCS010000000
VERSION     DAZPCS010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.486"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..>455)
                     /locus_tag="ACHUQ7_004427"
     CDS             complement(<1..>455)
                     /locus_tag="ACHUQ7_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1137480.1"
                     /translation="AKKRNIQTFLIFSLATILGGIYWYVRSFWISGDPFSPAGGNIFG
                     HYLWNEIDLQVQTAEQARHGISPATLDLQQAINKVGSDVVFYAILSVFTLRNNKILWV
                     FFSIVLTYVLFWFSVTQGDRYLSPIYPLSVLQVAISIASLIKDINITNY"
BASE COUNT          164 a           83 c           73 g          135 t
ORIGIN      
        1 ttgtaatttg taatgttgat atcttttatt aaacttgcaa ttgagatcgc aacctgcaat
       61 actgacaatg ggtaaatggg tgataaatac ctgtcacctt gcgtaacaga aaaccaaaat
      121 aagacatatg ttaaaacaat cgagaaaaat acccaaagta ttttattatt cctaagtgta
      181 aagacagaca gtatagcata aaaaacaaca tcactgccaa ctttattaat tgcttgttgc
      241 aaatctaatg tggcaggact tataccatgt cgagcttgtt ctgctgtttg aacttgtaga
      301 tcaatttcat tccataggta gtgaccaaat atatttcctc cagcaggact aaaaggatca
      361 ccagagatcc aaaaactacg gacataccaa taaatgccac caagaattgt tgccaagcta
      421 aaaataagaa atgtctgtat gtttctcttt ttcgc
//
LOCUS       DAZPCS010000477          450 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.487, whole genome shotgun sequence.
ACCESSION   DAZPCS010000477 DAZPCS010000000
VERSION     DAZPCS010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.487"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            1..149
                     /locus_tag="ACHUQ7_004428"
                     /pseudo
     CDS             1..149
                     /locus_tag="ACHUQ7_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            complement(169..>450)
                     /locus_tag="ACHUQ7_004429"
     CDS             complement(169..>450)
                     /locus_tag="ACHUQ7_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4/IS5 family transposase"
                     /protein_id="HFR1137481.1"
                     /translation="LRASKSRSTGRILVLSLLATLSTIVMWLLGYHAENKGLHLKYQA
                     NSIKSRRVISYLTLAKNVLRHSPLILRRTVLSTVLNHLSRTYRNMVLVY"
BASE COUNT          113 a          109 c          107 g          121 t
ORIGIN      
        1 cgcgcgaagt gttgtatgac aatatgaaaa ctgtggttct gcaacgtgac gcatatcaga
       61 ccggtcagca ccggttccat ccttcgctgt ggcagttcgg caaggagatg ggcttctctc
      121 cccgactgtg tcgccccttc aggctgaggg atccccacaa aattactgct aataaaccag
      181 caccatattt cggtaggttc tggacagatg gtttagaact gtgctcagta ctgttcgtct
      241 taaaataagt ggcgagtgtc gaagaacatt cttcgctaac gtcagatatg agataacccg
      301 ccgggattta atgctgttag cctgatattt caggtgtaac cctttatttt cagcgtgata
      361 accgagtaac cacattacga tagtgctcaa cgttgccagc agactcagaa ccagaattct
      421 tcctgttgaa cgacttttgc tggcccgaag
//
LOCUS       DAZPCS010000478          449 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.488, whole genome shotgun sequence.
ACCESSION   DAZPCS010000478 DAZPCS010000000
VERSION     DAZPCS010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.488"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..228
                     /locus_tag="ACHUQ7_004430"
     CDS             <1..228
                     /locus_tag="ACHUQ7_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393504.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HFR1137482.1"
                     /translation="ESNSDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLVNRNCYLD
                     KYGDARINASYTQLEIKHSQPKNRASGKRVS"
BASE COUNT          149 a           94 c           79 g          127 t
ORIGIN      
        1 gaaagcaatt ctgacagcgc cccctatacg ttattaaaaa actatgctga cagatttaaa
       61 aaatattcag cagaagagca gtacgaaatt gattttcaac atcgactggt aaacagaaat
      121 tgttatttag ataaatatgg cgatgcacga atcaatgctt cttatacaca attagaaata
      181 aaacactcac aacccaaaaa tagagcatcc ggcaaaagag tgagttaaca tcattgttaa
      241 tcaattcgac cagtcagtaa atatgctgac tggtcgaaca taatttgcta aaaagtcatc
      301 actgactgat gtcagtccca ccatctgcca gttcaatcta cgggtatttc cagcatatcg
      361 cggcacgcga ttgctggtct gttgatgtag tggattgtag ttgctgactt ttcttaccct
      421 cagatttctg ccccacttgt caattcaat
//
LOCUS       DAZPCS010000479          449 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.489, whole genome shotgun sequence.
ACCESSION   DAZPCS010000479 DAZPCS010000000
VERSION     DAZPCS010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.489"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..382)
                     /locus_tag="ACHUQ7_004431"
     CDS             complement(<1..382)
                     /locus_tag="ACHUQ7_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1137483.1"
                     /translation="MNVVSNTQLLEQRIADFFTLSDEHKKARVLLDTLACSCPARIFG
                     GMVRDLGLYGVDGFSSDLDIVIGRSREELFQTLAELPVKQLRFNKFGGIRFRYHDFEF
                     DIWNLNETWAFQEKLIFCEDESSLL"
BASE COUNT          135 a          106 c           96 g          112 t
ORIGIN      
        1 ttaacagact tgattcatcc tcacaaaata taagtttttc ctggaatgcc catgtctcat
       61 tgaggttcca gatatcaaat tcgaagtcgt gataacgaaa acggatccca ccaaacttat
      121 taaaacgcag ttgtttgacg ggcagttcag ccaatgtctg gaagagttcc tcacgggagc
      181 gaccgatgac gatatcaagg tctgaagaga atccatcgac gccatacaag cctaagtcac
      241 gcaccatccc gccaaaaatc ctggccggac aggaacaggc cagagtatca agtaatactc
      301 tggctttctt atgctcatca ctaagcgtaa aaaaatcggc gatacgctgt tcaagcagtt
      361 gggtgtttga gaccacattc atacagtgat tttagctcat cgtgaaggcg cattaaataa
      421 ctatccgtca tgcctgagat tgcgtcggt
//
LOCUS       DAZPCS010000480          443 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.490, whole genome shotgun sequence.
ACCESSION   DAZPCS010000480 DAZPCS010000000
VERSION     DAZPCS010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.490"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            213..>443
                     /locus_tag="ACHUQ7_004432"
     CDS             213..>443
                     /locus_tag="ACHUQ7_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFR1137484.1"
                     /translation="MTLPTTIYSFPAYLPRFSSTDKPAKLKFHQYARATLLSNRGRDH
                     NCDSRRTVEIHKLDLSDWQAFNKLATKCNAYDG"
BASE COUNT          141 a          105 c           61 g          136 t
ORIGIN      
        1 tatcgaacct cgcttaccaa cgcgttgtca gtcatctccc ccctctctgg tgcatcgtta
       61 tatccgtgat gagcgatttt tgatattaat atctttcacc atccgctatt ttcaccactt
      121 cagaaaacaa acttattttt attaaaatca attctttttc tttgaacatt caactgcgat
      181 atttgtaaaa acattaaaca ggcaacaata aaatgacatt accaacaact atttattcat
      241 tccccgcata tttaccccgt ttttcgtcca ccgacaaacc agcaaaatta aaatttcatc
      301 aatacgcacg cgctacatta ctgtcaaatc gtggaagaga tcataattgc gatagcagac
      361 gtactgttga gatccacaaa ctagatctct ctgactggca agcattcaat aagttagcaa
      421 cgaagtgtaa tgcttacgat gga
//
LOCUS       DAZPCS010000481          442 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.491, whole genome shotgun sequence.
ACCESSION   DAZPCS010000481 DAZPCS010000000
VERSION     DAZPCS010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.491"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..373
                     /locus_tag="ACHUQ7_004433"
     CDS             <1..373
                     /locus_tag="ACHUQ7_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HFR1137485.1"
                     /translation="MKELTGKALFMALRSQRLPAGLIHHTDRGSQYCAYDYRVIQEQS
                     GLKTSMSRKGNCYDNAPMESFWGTLKNESLSHYRFKSRDEAISVIREYIEIFYNRQRR
                     HSRLGNISPAAFRIKYYQMTA"
BASE COUNT          123 a          106 c          115 g           98 t
ORIGIN      
        1 gatgaaagag ttgacgggta aagccctgtt tatggcgctc aggagccagc gcctacctgc
       61 cgggctaatc caccacaccg atcgaggttc acagtactgc gcatacgatt acagggtcat
      121 acaggagcag tctggtctga aaacatcaat gtcgcgtaaa ggtaactgtt acgacaacgc
      181 tccgatggaa agcttctggg gaacgctgaa aaatgaaagc ctgagccact atcgttttaa
      241 gagccgggat gaggccatct cagtaatacg ggaatacatt gagattttct acaatcgtca
      301 gcgtcgtcac tctcgtctgg ggaatatctc cccggcagcc ttcaggataa aatattatca
      361 gatgactgct tagaaaaaga acaaatggtg gtgtccgcta ttgccagtac acctcaggcc
      421 atgtgcgcgg taactgaccg gg
//
LOCUS       DAZPCS010000482          439 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.492, whole genome shotgun sequence.
ACCESSION   DAZPCS010000482 DAZPCS010000000
VERSION     DAZPCS010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.492"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            11..>439
                     /gene="ospC2"
                     /locus_tag="ACHUQ7_004434"
     CDS             11..>439
                     /gene="ospC2"
                     /locus_tag="ACHUQ7_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HFR1137486.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSS
                     SGISEKHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYLSEIIQKTHPLSSDERHLLSIIINSDFNFRH"
BASE COUNT          153 a           82 c           69 g          135 t
ORIGIN      
        1 gggatgtttt atgaaaatac ctgaagcagt aaatcatatt aatgttcaga acaatattga
       61 tcttgttgat ggaaaaataa atccgaacaa agatacaaaa gcattacaaa aaaacatatc
      121 atgcgtaaca aactcatcct cttctggcat aagtgaaaaa catttagatc actgtgctga
      181 tactgtgaaa agctttttaa ggaagtctat agcagcgcaa agttatagta aaatgttctc
      241 tcaagggacc agtttcaaat cattaaatct ttctattgaa gcgccatctg gagcacggtc
      301 atcatttcgg tcacttgagc acctcgataa ggtgtccaga cactatcttt ctgaaataat
      361 acaaaaaact catcctcttt cttcggatga acgtcacttg ctctctatta ttattaattc
      421 tgattttaac ttcagacat
//
LOCUS       DAZPCS010000483          438 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.493, whole genome shotgun sequence.
ACCESSION   DAZPCS010000483 DAZPCS010000000
VERSION     DAZPCS010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.493"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..>438
                     /locus_tag="ACHUQ7_004435"
     CDS             <1..>438
                     /locus_tag="ACHUQ7_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFR1137487.1"
                     /translation="YASAGLLARILVSKYMEHIPLYRQSEIYARQGVELSRNTMVRWV
                     SEMADKLRPLYIALNDYVLEAGKVHADDTPVKVLAPGNGKTKTGRLWVYVRDDRNAGS
                     SLPAAVWFAYSADRKGEHPQLHLAKYQGVLQADAYAGYNVLYET"
BASE COUNT          113 a          103 c          129 g           93 t
ORIGIN      
        1 tatgccagtg cagggttact tgcacggatc ctggtcagca aatatatgga acatatccct
       61 ttatatcgcc agtcagaaat atacgcgcga cagggcgtgg agctgagccg taataccatg
      121 gtgcgctggg tatcagaaat ggcagacaaa ctccgtcctc tgtatatagc gctgaatgac
      181 tatgttctgg aggcaggaaa ggtgcacgca gatgacactc cggtgaaagt actggccccg
      241 gggaacggaa agacgaaaac gggtcgtctg tgggtatacg tcagggatga tcgtaatgcg
      301 ggttcatccc tgccggcagc cgtctggttc gcgtattcgg cagatcgcaa aggagaacat
      361 ccgcagctcc acctggcaaa gtatcagggc gtactgcagg ctgatgccta tgcaggttat
      421 aacgtactgt acgaaacg
//
LOCUS       DAZPCS010000484          435 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.494, whole genome shotgun sequence.
ACCESSION   DAZPCS010000484 DAZPCS010000000
VERSION     DAZPCS010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.494"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..269
                     /locus_tag="ACHUQ7_004436"
     CDS             <1..269
                     /locus_tag="ACHUQ7_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR1137488.1"
                     /translation="SQYEDGLLSDDDIQECMPVPVALTDTPEPTRPVTNPFWRNKHDE
                     RPEGAPGNVPRCELHLKSGVVKLFDPLTPELLRALIREMKGGIR"
     gene            266..>435
                     /gene="tnpB"
                     /locus_tag="ACHUQ7_004437"
     CDS             266..>435
                     /gene="tnpB"
                     /locus_tag="ACHUQ7_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858246.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR1137489.1"
                     /translation="MITLPTGTKIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFRGRSGKMVKIL"
BASE COUNT          109 a          114 c          122 g           90 t
ORIGIN      
        1 aaagccagta cgaggacggc ttactgagcg atgatgacat acaggaatgc atgcctgtcc
       61 cggtggcact gactgatacg ccggagccga ccagaccagt tacaaatccc ttctggcgta
      121 acaagcatga tgagcgccct gagggggctc ccggaaacgt cccacggtgc gagctgcatc
      181 ttaaatcagg tgtggtaaaa ctgtttgacc ctctcactcc ggaactgtta cgggcgctaa
      241 tccgcgaaat gaaagggggt atccgatgat aacgctgccg accggtacca aaatctggat
      301 catcgctggc atcacagata tgcgttgtgg cttcaatggc ctggcttcga aggtgcagaa
      361 cacgctgaaa gatgacccgt tctccgggca tatcttcgtc ttccggggcc gcagtggcaa
      421 aatggtgaaa atact
//
LOCUS       DAZPCS010000485          434 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.495, whole genome shotgun sequence.
ACCESSION   DAZPCS010000485 DAZPCS010000000
VERSION     DAZPCS010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.495"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            35..434
                     /locus_tag="ACHUQ7_004438"
                     /pseudo
     CDS             35..434
                     /locus_tag="ACHUQ7_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          100 a          112 c          114 g          108 t
ORIGIN      
        1 atttagtgta tgatggtgat tttaaggtgc ttgcgtggct tccatttcca tcagatgtcc
       61 ttcctgctcc gctactgaag gcgtggtgcg taacggcaaa agcactgccg gacatcagcg
      121 ctatctctgc tctcattgcc gtaaaacatg gcaactacag ttcacttaca ccgcctctca
      181 gcccggtacg caccagaaaa tcattgatat ggccatgaat ggcgtcggat gtcgcgccag
      241 tgcacgcatt atgggcgttg gcctcaacac ggttttacgt cacttaaaaa actcaggccg
      301 cagtcggtaa cctcgcgcat acaaccgggc agtgatgtga ttgtctgcgc tgaaatggac
      361 gaacattggg gctacgtcgg tgctaaatca cgtcagcgct ggctgtttta cgcgtatgac
      421 aggatacgga ggac
//
LOCUS       DAZPCS010000486          424 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.496, whole genome shotgun sequence.
ACCESSION   DAZPCS010000486 DAZPCS010000000
VERSION     DAZPCS010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.496"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
BASE COUNT          108 a           90 c           83 g          143 t
ORIGIN      
        1 cactttcttg tatgtattat agctaaatga acagatttta atatgttgta tggttttcac
       61 tctgaaatcc cctgaattta atcacgtttc ctttactgaa ggcaatttta accgtagtat
      121 caattattaa cccttgggct tgatatccct ctcctcttct gggcacgggg gatgtcagat
      181 tacttattac agaaaccaaa agaattgtaa gcgtcatctc agtgtcgtct atttcaatgt
      241 ctgatgattg gcgatgatag agtcctctta taacgttaat ggactctatc aatgtcagat
      301 tctcttcgcc cccgcaggat tcgtgcctct tactctatga ctctatggag tttaagcttg
      361 atcttattga aaaatcttac cagctagggg cctgcgtggc ccaactggcc agagagtacg
      421 ggat
//
LOCUS       DAZPCS010000487          416 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.497, whole genome shotgun sequence.
ACCESSION   DAZPCS010000487 DAZPCS010000000
VERSION     DAZPCS010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.497"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..373
                     /gene="istB"
                     /locus_tag="ACHUQ7_004439"
     CDS             <1..373
                     /gene="istB"
                     /locus_tag="ACHUQ7_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HFR1137490.1"
                     /translation="TAADLLLQLSTAQRQGRYKTTLQRGVMAPRLLIIDEIGYLPFSQ
                     EEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDAALTSAMLGRILHHSHVVQIK
                     GESYRLRQKRKAGVIAEANPE"
BASE COUNT          121 a           99 c          105 g           91 t
ORIGIN      
        1 aacagcagca gatctgttac ttcagttatc tacggcacaa cgtcagggcc gttataaaac
       61 gacgcttcag cgtggagtaa tggccccccg cctgctcatc attgatgaaa taggctatct
      121 gccgttcagt caggaagaag caaaactgtt cttccaggtc atcgctaaac gttacgaaaa
      181 gagcgcaatg atcctgacat ccaatctacc gttcgggcag tgggatcaaa cgttcgccgg
      241 tgatgcagca ctgacctcag cgatgctggg ccgtatctta caccactcac atgtcgttca
      301 aatcaaagga gaaagctatc gactcagaca gaaacgaaag gccggggtta tagcagaagc
      361 taatcctgag taaaacggtg gatcaatatt gggccgttgg tggagatata agtgga
//
LOCUS       DAZPCS010000488          414 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.498, whole genome shotgun sequence.
ACCESSION   DAZPCS010000488 DAZPCS010000000
VERSION     DAZPCS010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.498"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..>414
                     /locus_tag="ACHUQ7_004440"
     CDS             <1..>414
                     /locus_tag="ACHUQ7_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001400151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HFR1137491.1"
                     /translation="SVGLVLDDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFA
                     DFNPSKRPWSVASDDSNNSWNPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDL
                     NWRYLNEYLDQISANDEVLFLKQGHEIIAKNQLARE"
BASE COUNT          120 a           77 c           96 g          121 t
ORIGIN      
        1 tcggtgggac tggtacttga tgataataaa tattatctct tttcgcggag ggcgaatgat
       61 aaaatcgttg tttatcatca ggaacaagta aatggaccgc ttgtcgacga gtcagggcgg
      121 gttatttttg ccgattttaa cccatcgaaa cgaccgtggt cggtggcttc agatgactct
      181 aacaacagct ggaatccggc atacaattgc tttgatcgtc cgggtaaaaa atgtatctct
      241 tttacgctac gtatcaacgg caaagatcac gatttgttag cggtagataa aattcatgtc
      301 gatttaaact ggcggtatct gaacgagtat cttgatcaaa tcagcgctaa tgatgaagtt
      361 ctatttttga aacaaggtca tgagatcatt gccaagaatc aactcgctcg tgaa
//
LOCUS       DAZPCS010000489          410 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.499, whole genome shotgun sequence.
ACCESSION   DAZPCS010000489 DAZPCS010000000
VERSION     DAZPCS010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.499"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            156..341
                     /locus_tag="ACHUQ7_004441"
                     /pseudo
     CDS             156..341
                     /locus_tag="ACHUQ7_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          119 a           97 c          108 g           86 t
ORIGIN      
        1 atacggtggt cggtttacgc ttaccgcagg acaggtggta actgaaaaat agtttgctga
       61 ggcgactgga aacagaaggg caagatcgat tccgacagca gtgatggtca gctacctcat
      121 cgataacttt gcaacagtgc ccttcttgaa gcatcatgct tcatgaagaa aaactggcac
      181 gtcatcaacg taaacaggcg atgtataccc gaatggcagc cttcccggcg gtgaaaacgt
      241 tcgaagagta tgacttcacg ttcgccaccg gagcaccgca gaagcagctc cagtcgttac
      301 gctcactcag cttcatagag cgcaatgaaa acatagtttt acaggggaca tctgacatca
      361 caaatccccg ggtggggatt tgtgtataag agacagccca tggaggaagg
//
LOCUS       DAZPCS010000490          401 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.500, whole genome shotgun sequence.
ACCESSION   DAZPCS010000490 DAZPCS010000000
VERSION     DAZPCS010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.500"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..>401
                     /locus_tag="ACHUQ7_004442"
     CDS             <1..>401
                     /locus_tag="ACHUQ7_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1137492.1"
                     /translation="FSTNLIQSNYGDLNIKSLAFDSFKERLESTMAALTFFISTGQCD
                     CDEIAESNFNRMIAYMSNINYDASKLGAPALSFDTYLQDNVKYRVIINNLYGSEFRIR
                     DINENFVGMDFTCEFRPEKMTNLINIKNRLV"
BASE COUNT          131 a           60 c           81 g          129 t
ORIGIN      
        1 gttttctaca aacttaatac agagtaatta tggtgattta aatattaaaa gcctggcttt
       61 cgactctttt aaagaaaggc ttgagtctac catggcggca ctcactttct ttatatcaac
      121 gggtcaatgt gactgcgatg aaatagcaga gtcgaacttc aatcgtatga ttgcctatat
      181 gtctaacatt aactatgatg cctcaaagct aggagcgcca gcacttagtt ttgatacgta
      241 tctgcaggac aatgttaaat atagagtgat aattaataat ctttatggca gtgagtttcg
      301 gataagagat attaatgaga attttgttgg gatggatttt acctgtgaat tccgacccga
      361 aaaaatgacg aatttgatca atataaaaaa taggttggtt a
//
LOCUS       DAZPCS010000491          395 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.501, whole genome shotgun sequence.
ACCESSION   DAZPCS010000491 DAZPCS010000000
VERSION     DAZPCS010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.501"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..64)
                     /locus_tag="ACHUQ7_004443"
     CDS             complement(<1..64)
                     /locus_tag="ACHUQ7_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1137493.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVV"
     gene            complement(320..>395)
                     /locus_tag="ACHUQ7_004444"
     CDS             complement(320..>395)
                     /locus_tag="ACHUQ7_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HFR1137494.1"
                     /translation="LEQLINVNFFSSDRTSFCECNRFP"
BASE COUNT          119 a           86 c           83 g          107 t
ORIGIN      
        1 taaccactgc gtgcattggc gggattgaag ggtgaaaaat atgtgcaatt gggaagcctt
       61 tcattgatgc ctgccttgtc gtcatgacaa acaataatag atgcttattt tcctcgcacg
      121 acacttacgc actatcaaaa aacgctcatt tacccggcgt tacagtaaac gaagcaacat
      181 tctgtgtcct acgctgatgt aaagcacagt gaaaatcccc cttgttgact tcgtctgacc
      241 ttatcgcggc gaccagcaat tattcgtttt gtgatcctca acacaaaatg ctattcgtcg
      301 cctatgaggg gagaagaatt tatggaaaac gattgcattc acaaaatgac gtcctgtcag
      361 aagagaaaaa gttgacgtta atcaattgtt cgaga
//
LOCUS       DAZPCS010000492          390 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.502, whole genome shotgun sequence.
ACCESSION   DAZPCS010000492 DAZPCS010000000
VERSION     DAZPCS010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.502"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(42..>390)
                     /locus_tag="ACHUQ7_004445"
     CDS             complement(42..>390)
                     /locus_tag="ACHUQ7_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1137495.1"
                     /translation="QIKPFLEKAMTMYNDVLFPGEDINKPISIAAANRFVNRIRGGMD
                     LGYWRTHDFRRTLVTRLSEMNVEPHVTERMLGHELGGIMSVYNKHDWIEAQRKAYELH
                     ADKLFWHIRSISD"
BASE COUNT           92 a           91 c           87 g          120 t
ORIGIN      
        1 ccttcgtgaa gctgttgaag ggtggattct taacggtggc gttaatcaga aatgctcctg
       61 atgtgccaga acaatttatc agcgtgaagc tcatacgctt tgcgctgagc ctctatccag
      121 tcgtgtttat tgtatacgga cattatcccg ccaagttcat gaccgagcat tcgctcagta
      181 acatgaggct cgacattcat ctcggacagc cgtgtaacaa gcgttcttct gaaatcatgt
      241 gttcgccagt aacctagatc catccctccc cttattctat ttacgaatcg gttggctgca
      301 gcgatgctga tcggtttgtt tatgtcttct ccagggaaaa gaacatcatt gtacattgtc
      361 atggcctttt cgagaaaagg cttaatttgt
//
LOCUS       DAZPCS010000493          381 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.503, whole genome shotgun sequence.
ACCESSION   DAZPCS010000493 DAZPCS010000000
VERSION     DAZPCS010000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.503"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            1..381
                     /locus_tag="ACHUQ7_004446"
                     /pseudo
     CDS             1..381
                     /locus_tag="ACHUQ7_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          129 a           93 c           47 g          112 t
ORIGIN      
        1 tcaaaacgat atcattatca gaactcatta taagtctcct catagattgc acatcgatag
       61 cgacatacca acaccttcat cagagcctat taataaattt gcgcgccagc tcatcaccct
      121 acttgatacc tctgacttaa gttcgatgct gtcatactgt gttactcagg aatttaccgc
      181 aaactgtcga aaatatcaca aaattgttat tccactgccc tttttaccat taactttgcc
      241 acttcaccca tccatgcaga aaatatactc attacattac actataaaaa agaaatcatt
      301 tccttattac tagaaaccac gcctattaaa gctaaccatt tgcgaagcat actggattat
      361 attgaacagg aacagggtaa t
//
LOCUS       DAZPCS010000494          380 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.504, whole genome shotgun sequence.
ACCESSION   DAZPCS010000494 DAZPCS010000000
VERSION     DAZPCS010000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.504"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(1..248)
                     /locus_tag="ACHUQ7_004447"
                     /pseudo
     CDS             complement(1..248)
                     /locus_tag="ACHUQ7_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           87 a          121 c           86 g           86 t
ORIGIN      
        1 ctgtctccgt tatggcgtac accacatctt ccagtaatcg ccgcttcacc ggactcagga
       61 ccccgtatca ccatccggaa ccccttatcc gccacatgcc aggagaacct ttccataaac
      121 tcgtcgccac tcatcacaag gttttcttct cgttttgtcc ggtggctgtt gtaacgcagg
      181 ccaatttcat cctgaccggc ataatgttcc aggcggctca tctgcaccgg ctgcttctcc
      241 agccagttaa gccatcggtt tccctgctga aaaatatcgg caaaacgggg aagcatcaga
      301 aaggcggaat aacgccatcc ggccagtgaa ccgtaccgca ttcagggcaa tacatgccgt
      361 cggcgttgat accggcagga
//
LOCUS       DAZPCS010000495          375 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.505, whole genome shotgun sequence.
ACCESSION   DAZPCS010000495 DAZPCS010000000
VERSION     DAZPCS010000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.505"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..>375
                     /locus_tag="ACHUQ7_004448"
     CDS             <1..>375
                     /locus_tag="ACHUQ7_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR1137496.1"
                     /translation="DGRRSRHSDDTDVLLRIHHVIGELPTYGYRRVWALLRRQAELDG
                     MPAINAKRIYRLMRQNALLLERKLAVPPSKRAHTGRVAVKESNQRWCSDGFEFRCDNG
                     EKLRVTFALDCCDRKALHWAVTT"
BASE COUNT           86 a           96 c          110 g           83 t
ORIGIN      
        1 agatggtcgc cgcagccgtc actcagatga tacggatgtg cttctccgta tacaccatgt
       61 tatcggagag ctgcccacat atggttatcg tcgggtatgg gcgctgcttc gcagacaggc
      121 cgaacttgat ggtatgcctg cgatcaatgc caaacgtatt taccggctca tgcgccagaa
      181 tgcgctgttg cttgagcgaa aactcgctgt accgccatcg aaacgggcac ataccggcag
      241 agtggctgtg aaagaaagta atcagcgatg gtgctctgac gggttcgagt tccgctgtga
      301 taacggagaa aaactgcgag tcacgttcgc gctggactgc tgtgaccgta aggcactgca
      361 ctgggcggtc actac
//
LOCUS       DAZPCS010000496          372 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.506, whole genome shotgun sequence.
ACCESSION   DAZPCS010000496 DAZPCS010000000
VERSION     DAZPCS010000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.506"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..311)
                     /locus_tag="ACHUQ7_004449"
     CDS             complement(<1..311)
                     /locus_tag="ACHUQ7_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1137497.1"
                     /translation="MNSQTTKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFLASGIAWPLPDSVSFAQLDAILYANRKKELTEPQIREGSWRKERRTSYSRE
                     F"
BASE COUNT           99 a           74 c           96 g          103 t
ORIGIN      
        1 ttaaattcac ggctatagct ggttcgccgt tcttttcgcc atgagccttc tctgatttga
       61 ggctctgtta attccttctt tctgttggca taaaggatgg cgtcaagttg agcgaatgaa
      121 actgaatcgg gcaatggcca tgcgataccg gatgcaagaa atcgctgaaa aagcgtatgt
      181 attgtggaat gactgagacc cagacgctga gcgatggccc ggatggtcag tttatcttca
      241 aatcttaaac gcagggcatc aggcaaataa gaacggaagc agggaatatc ttttgttgtc
      301 tgggaattca tcgttcgtgt ccatcaatat agatgggcgc gattgttgcc agacaggaca
      361 attttcacaa ga
//
LOCUS       DAZPCS010000497          371 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.507, whole genome shotgun sequence.
ACCESSION   DAZPCS010000497 DAZPCS010000000
VERSION     DAZPCS010000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.507"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            83..>371
                     /locus_tag="ACHUQ7_004450"
     CDS             83..>371
                     /locus_tag="ACHUQ7_004450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1137498.1"
                     /translation="MDEIRIRIAGNTAAPCFHVLKNKGYKVTMETHALSDDCLDCVYD
                     WYAEKNGQFFSATSPEELLGLVAMWEVRGNDWRANLNEINTYQKAIEESPFF"
BASE COUNT          125 a           41 c           93 g          112 t
ORIGIN      
        1 ctaggaataa tgaggtgtta gttcatgggc aaattcttgg aaataaagtt agccgatgta
       61 attagttagt ggagaggaag ttgtggacga aattagaata agaattgctg gtaatacggc
      121 tgcgccttgt ttccatgtgc taaaaaataa gggatataaa gtaacaatgg aaactcatgc
      181 attatctgac gattgtttag attgtgtata tgattggtat gcggaaaaaa atggacaatt
      241 ttttagtgca acgagtccag aagagttact tggattagtt gcaatgtggg aagttcgtgg
      301 taatgactgg agagctaatt tgaatgagat aaatacttat caaaaagcca tagaagaatc
      361 cccgtttttt g
//
LOCUS       DAZPCS010000498          366 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.508, whole genome shotgun sequence.
ACCESSION   DAZPCS010000498 DAZPCS010000000
VERSION     DAZPCS010000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.508"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            233..325
                     /locus_tag="ACHUQ7_004451"
                     /pseudo
     CDS             233..325
                     /locus_tag="ACHUQ7_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418245.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          105 a           89 c           60 g          112 t
ORIGIN      
        1 actaaaaaca gcatcaataa tcaacgcgat ataataaacc tgccttacat atcaactgcg
       61 ccagaggtag gattgaaaac gctctcctga ttttccaatt cattttctgg ataaataaat
      121 aatttatttt tgtcactatt atttatgtaa tcatcctgtc agggagaggg atctcaatta
      181 tcaatgctta attacgtcat cattttgatg acatgaaacc ggagcatttt gcatggaatt
      241 ttcgttttca cccaaacgtc ttgttgttgc tgtcgccgcc gctcttcctc tcatggccag
      301 cgcagcagat accccgtcaa ctgctagact ggccccctga atctccagac aaccagtatc
      361 acttaa
//
LOCUS       DAZPCS010000499          365 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.509, whole genome shotgun sequence.
ACCESSION   DAZPCS010000499 DAZPCS010000000
VERSION     DAZPCS010000499.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.509"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..>365
                     /gene="ospC1"
                     /locus_tag="ACHUQ7_004452"
     CDS             <1..>365
                     /gene="ospC1"
                     /locus_tag="ACHUQ7_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HFR1137499.1"
                     /translation="PIFNTKDMKLGLGLYLIDFIRKSEDQSFKEFCYGKNLAPVDLDR
                     IINFVFQPEYHIPRMVSTENFKKVKIREISLEEAVTASNYEEINKQVTNKKIALQALF
                     LSITNQKEDVALYILSNFE"
BASE COUNT          133 a           58 c           65 g          109 t
ORIGIN      
        1 taccaatatt caatactaaa gatatgaagt tagggctcgg attatacctg attgacttta
       61 ttagaaaaag tgaagaccaa agcttcaagg agttttgcta tggaaaaaat cttgcccctg
      121 tggatctgga tagaatcata aactttgttt ttcagccaga gtaccatata cctaggatgg
      181 taagtacaga aaacttcaaa aaagttaaga ttagagaaat atccttagag gaggctgtta
      241 cagcatctaa ttacgaagaa attaacaagc aggtcactaa caaaaaaatt gctctccagg
      301 ctctttttct ttcgattact aatcaaaaag aggatgtcgc cttatatata ttatctaatt
      361 ttgag
//
LOCUS       DAZPCS010000500          365 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.510, whole genome shotgun sequence.
ACCESSION   DAZPCS010000500 DAZPCS010000000
VERSION     DAZPCS010000500.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.510"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..>365
                     /locus_tag="ACHUQ7_004453"
     CDS             <1..>365
                     /locus_tag="ACHUQ7_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HFR1137500.1"
                     /translation="HQIVNRLDIMLPGNRPFVAPSAVIQARQRLGSEAVRRVFTKTAQ
                     LWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTHAGNPALYPQVKMVCQME
                     LTSHLLTAAAFGTMKNSEN"
BASE COUNT           76 a          124 c          102 g           63 t
ORIGIN      
        1 tcaccagatt gtgaatcgcc tggacatcat gctgccgggc aatcgcccct tcgttgcccc
       61 cagtgccgtt attcaggccc gccagcgcct gggaagtgag gctgtccgcc gcgtgttcac
      121 gaaaacagcg cagctctggc ataacgccac gccgcatccg cactggtgcg gcctgaccct
      181 gctggccatc gatggtgtgt tctggcgcac accggataca ccagagaacg atgcagcctt
      241 cccccgccag acacatgccg ggaacccggc gctctacccg caggtcaaaa tggtctgcca
      301 gatggaactg accagccatc tgctgacggc tgcagccttc ggcacgatga agaacagcga
      361 aaatg
//
LOCUS       DAZPCS010000501          360 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.511, whole genome shotgun sequence.
ACCESSION   DAZPCS010000501 DAZPCS010000000
VERSION     DAZPCS010000501.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.511"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..>360
                     /locus_tag="ACHUQ7_004454"
     CDS             <1..>360
                     /locus_tag="ACHUQ7_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFR1137501.1"
                     /translation="WTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTMKALEMAWET
                     RGKPGGVMFHSDQGSHYTSRQFRQLLWRYQIRQSMSRRGNCWDNSPMERFFRSLKNEW
                     MPVVGYVSFSEAAHAIT"
BASE COUNT           85 a           89 c          118 g           68 t
ORIGIN      
        1 ctggacgggt aagcgctggg cgtacctcgc cgttgttctc gacctgttcg caagaaaacc
       61 agtgggctgg gccatgtcgt tctcgccgga cagcaggctc accatgaaag cgctggaaat
      121 ggcatgggaa acccgtggta agcccggcgg ggtgatgttc cacagcgatc agggcagtca
      181 ttatacgagc aggcagttcc ggcagttatt gtggcgatac cagatcagac agagtatgag
      241 ccggcgcgga aactgctggg ataacagccc aatggaacgc ttcttcagga gtctgaagaa
      301 cgaatggatg ccggtggtgg gttacgtaag cttcagcgag gcagctcacg ccataacgga
//
LOCUS       DAZPCS010000502          353 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.512, whole genome shotgun sequence.
ACCESSION   DAZPCS010000502 DAZPCS010000000
VERSION     DAZPCS010000502.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.512"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..264
                     /locus_tag="ACHUQ7_004455"
     CDS             <1..264
                     /locus_tag="ACHUQ7_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR1137502.1"
                     /translation="FYRNDEFVFDYRLKAEDERDALLAQVSARTGEWEEDLLLGLISD
                     EDKEKLKACRIYAKSLQAMDFSTITDKSSYNAIEWPASPEGSS"
     gene            288..>353
                     /locus_tag="ACHUQ7_004456"
     CDS             288..>353
                     /locus_tag="ACHUQ7_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR1137503.1"
                     /translation="MSVVISGALTDGAGIPMSGYHI"
BASE COUNT           96 a           80 c           90 g           87 t
ORIGIN      
        1 ttttaccgca atgatgaatt tgtctttgac tacagactca aagcggaaga tgagcgtgat
       61 gcccttctgg ctcaggtcag tgcccggaca ggggaatggg aagaagacct gctgctgggg
      121 ttaatcagcg acgaagataa agaaaagctg aaagcctgtc gtatttacgc gaaatcgctg
      181 caggcgatgg atttcagcac catcactgat aaatcctcat acaacgccat tgaatggccc
      241 gcctctccgg aaggttcttc ctgatttaat ttatcgcgag aaaaacaatg tctgtagtga
      301 tatcaggtgc gctgactgat ggcgcaggta tccccatgtc cggataccat att
//
LOCUS       DAZPCS010000503          349 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.513, whole genome shotgun sequence.
ACCESSION   DAZPCS010000503 DAZPCS010000000
VERSION     DAZPCS010000503.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.513"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            1..349
                     /locus_tag="ACHUQ7_004457"
                     /pseudo
     CDS             1..349
                     /locus_tag="ACHUQ7_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS1-like element IS1B family transposase"
BASE COUNT           76 a           91 c          107 g           75 t
ORIGIN      
        1 aacccggtac gcaccagaaa atcattgata tggccatgaa tggcgttgga tgccgggcaa
       61 ccgcccgcat tatgggcgtt ggcctcaaca cgattttccg ccatttaaaa aactcaggcc
      121 gcagtcggta acctcgcgca tacagccggg cagtgacgtc atcgtctgcg cggaaatgga
      181 cgaacagtgg ggatacgtcg gggctaaatc gcgccagcgc tggctgtttt acgcgtatga
      241 caggctccgg aagacggttg ttgcgcacgt attcggtgaa cgcactatgg cgacgctggg
      301 gcgtcttatg agcctgctgt caccctttga cgtggtgata tggatgacg
//
LOCUS       DAZPCS010000504          345 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.514, whole genome shotgun sequence.
ACCESSION   DAZPCS010000504 DAZPCS010000000
VERSION     DAZPCS010000504.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.514"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(13..285)
                     /locus_tag="ACHUQ7_004458"
     CDS             complement(13..285)
                     /locus_tag="ACHUQ7_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1137504.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYKSVFADFITGHPSC
                     TVCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
BASE COUNT           80 a           81 c           90 g           94 t
ORIGIN      
        1 cggggctgaa aatcaccacc acttcgggtc atccgccttc atctccgctt ctgtttcgta
       61 taaatcaaaa cgacggcagg tatggcagaa cgtgacgtat tcgtacggtg aatccgggta
      121 cattttgtgg aaggtttccc agaaacagac cgtgcaggag ggatgaccgg tgataaagtc
      181 tgcaaatacg gacttataag ggtgattatt ggctctggcg acggctttca gaacctcttt
      241 caccattctg gtgtggactt tctggtgctc caggttgtgt gacatgggaa ctcattctgg
      301 atggttactc tgaaagctca tattctgcca caccccgatt tgcag
//
LOCUS       DAZPCS010000505          345 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.515, whole genome shotgun sequence.
ACCESSION   DAZPCS010000505 DAZPCS010000000
VERSION     DAZPCS010000505.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.515"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
BASE COUNT           98 a           88 c           71 g           88 t
ORIGIN      
        1 tctattatta ataccgtaga tattcatctt accctgtgca ccaaaagcaa aaacttatcg
       61 acgcgggttt gaaactgatg gcaaccgcaa aaatgcctga tgcgctacgc ttatcaggcc
      121 tacgccatct ctgcaatata ttgaatttgc gtgcttttgt aggcaggata aggcgttcac
      181 gcagcatccg gcatgaataa agcgcactca acaatctgaa acccgccgga acggtttatt
      241 acgtacatca ggtgaaactg accgataagc cgctttcttt tgggtatagt gtcgtggaca
      301 gtcattcatc tttctgcccc tccaaaagca aaaacccgcc gaagc
//
LOCUS       DAZPCS010000506          344 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.516, whole genome shotgun sequence.
ACCESSION   DAZPCS010000506 DAZPCS010000000
VERSION     DAZPCS010000506.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.516"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..>344
                     /locus_tag="ACHUQ7_004459"
     CDS             <1..>344
                     /locus_tag="ACHUQ7_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR1137505.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAKILEL"
BASE COUNT           77 a           88 c          105 g           74 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaaatcctgg aact
//
LOCUS       DAZPCS010000507          342 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.517, whole genome shotgun sequence.
ACCESSION   DAZPCS010000507 DAZPCS010000000
VERSION     DAZPCS010000507.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.517"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            2..172
                     /gene="xisR"
                     /locus_tag="ACHUQ7_004460"
     CDS             2..172
                     /gene="xisR"
                     /locus_tag="ACHUQ7_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFR1137506.1"
                     /translation="MMLRTGLGARQIEAYRQNCWVEGFHFKRVSPLGKPDSKRGIIWY
                     NYPKINQFIKDS"
     gene            174..>342
                     /locus_tag="ACHUQ7_004461"
     CDS             174..>342
                     /locus_tag="ACHUQ7_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HFR1137507.1"
                     /translation="MSKLPTGVEIRGKYIRIWFMFRGKRCRETLKGWEITNSNIKKAG
                     NLRALIVHQISS"
BASE COUNT          108 a           65 c           83 g           86 t
ORIGIN      
        1 cctgatgctt cgcactggtc tgggtgccag acaaattgaa gcataccgcc agaactgttg
       61 ggtggaaggc ttccacttca aacgagtatc tcctttaggg aagccagaca gtaagcgagg
      121 gattatctgg tacaactatc cgaagataaa tcagtttatc aaagactcat gatatgtcta
      181 aattaccaac aggtgtcgag attcgaggta aatacattcg catctggttc atgtttcgag
      241 gaaaacgatg tcgggaaaca ttgaaaggct gggagattac taacagtaac attaaaaagg
      301 ccgggaattt aagagcgttg atagttcatc aaatcagttc cg
//
LOCUS       DAZPCS010000508          338 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.518, whole genome shotgun sequence.
ACCESSION   DAZPCS010000508 DAZPCS010000000
VERSION     DAZPCS010000508.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.518"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..>338
                     /locus_tag="ACHUQ7_004462"
     CDS             <1..>338
                     /locus_tag="ACHUQ7_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1137508.1"
                     /translation="NGKVIAPACTLVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVP
                     IALEGCDVTVTKNATFTFSGTADGVQPTAFANQATTDAATNVALQMYLPDGSTSVTPG
                     TETSNIQLAD"
BASE COUNT           93 a           79 c           78 g           88 t
ORIGIN      
        1 taatggtaaa gttattgctc cagcttgtac cctggtagct gcgacgaaag attcagtagt
       61 cacattgcca aatgtcagcg ccaccaagtt gcaaactaat ggagcagtct ctggcgttaa
      121 aactgatgta ccaattgcct tagaaggctg tgatgttact gtaactaaaa atgcgacgtt
      181 tacctttagt ggtacggctg acggcgtgca acctacagca tttgccaacc aggctaccac
      241 cgatgcagcg acgaatgttg ctctacaaat gtatctgcca gatggttcta catcggtaac
      301 tcccggtacc gaaacgtcga atattcagtt agcggatt
//
LOCUS       DAZPCS010000509          337 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.519, whole genome shotgun sequence.
ACCESSION   DAZPCS010000509 DAZPCS010000000
VERSION     DAZPCS010000509.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.519"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..>337
                     /locus_tag="ACHUQ7_004463"
     CDS             <1..>337
                     /locus_tag="ACHUQ7_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFR1137509.1"
                     /translation="YREIKQTMQLSRLTLRSKKPELVEQELWGVLLAYNLVRYQMIKM
                     AEHLKGYWPNQLSFSESCGMVMRMLMTLQGASPGRIPELMRDLASMGQLVKLPTRRGR
                     AFPRVVKERP"
BASE COUNT          100 a           59 c          108 g           70 t
ORIGIN      
        1 gatacaggga gataaaacag acgatgcaac tgagcaggct gacgctgaga agtaaaaagc
       61 cggagcttgt ggagcaagag ctgtggggtg tcttactggc ttataatctg gtgagatatc
      121 agatgattaa aatggcggaa catctgaaag gttactggcc gaatcaactg agtttctcag
      181 aatcatgcgg aatggtgatg agaatgctga tgacattgca gggcgcttca ccgggacgta
      241 taccggagct gatgcgcgat cttgcaagta tgggacaact tgtgaaatta ccgacgagaa
      301 ggggaagggc cttcccgaga gtggtaaagg agaggcc
//
LOCUS       DAZPCS010000510          336 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.520, whole genome shotgun sequence.
ACCESSION   DAZPCS010000510 DAZPCS010000000
VERSION     DAZPCS010000510.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.520"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..>336)
                     /locus_tag="ACHUQ7_004464"
     CDS             complement(<1..>336)
                     /locus_tag="ACHUQ7_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1137510.1"
                     /translation="SRDNLGLKSAATMEAQSDIYDRTKGRLAIPGAFGFGCAFLPEDV
                     IRFDTKSDFLAWVRNALPGEYSVAGPYGIIIPDTRFEGVLSIRWTDARPETTEPRYRA
                     KSLTFYGING"
BASE COUNT           76 a           95 c           88 g           77 t
ORIGIN      
        1 gccgttaatg ccgtaaaaag taagggattt ggctctgtac cgcggttctg ttgtctcagg
       61 gcgtgcatca gtccaccgga tgctgagcac cccttcaaac cgtgtgtcgg gtatgatgat
      121 gccgtagggg ccagcaacgg aatattcacc tggcagcgca ttccttaccc aggccaggaa
      181 atcactctta gtgtcaaaac ggataacatc ttcaggcaga aaagcacacc caaagccgaa
      241 tgcgccgggt atcgccagac ggccttttgt ccggtcgtaa atgtcgctct gtgcttccat
      301 cgtggccgca cttttcagcc ccagattatc ccggga
//
LOCUS       DAZPCS010000511          334 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.521, whole genome shotgun sequence.
ACCESSION   DAZPCS010000511 DAZPCS010000000
VERSION     DAZPCS010000511.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.521"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            257..>334
                     /locus_tag="ACHUQ7_004465"
     CDS             257..>334
                     /locus_tag="ACHUQ7_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR1137511.1"
                     /translation="MSIRCPSCSATEGVVRNGKSTAGHQR"
BASE COUNT          107 a           59 c           72 g           96 t
ORIGIN      
        1 cctttactga aaggaattat tgcaacagta ttatttgttt ttggatatat atcttgcgcc
       61 ttgttatgta gttaatgctc agaatatgat agataaaata gagacgcaaa acagggcatg
      121 ctcataacat gatgataaca taaaaaatta gtaacaataa atgaaagaat catgggcgct
      181 caacaaaaag agggtaatgc tgccaactta ctgatttagt gtatgatggt gattttaagg
      241 tgcttgcgta gcttccattt ccatcagatg tccttcctgc tccgctactg aaggcgtggt
      301 gcgtaacggc aaaagcactg ccggacatca gcgc
//
LOCUS       DAZPCS010000512          333 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.522, whole genome shotgun sequence.
ACCESSION   DAZPCS010000512 DAZPCS010000000
VERSION     DAZPCS010000512.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.522"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(43..330)
                     /locus_tag="ACHUQ7_004466"
     CDS             complement(43..330)
                     /locus_tag="ACHUQ7_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1137512.1"
                     /translation="MDEEYRKDLQLWFGLTHASFCVMPRVFMEAMPPEWQEKMAQLLF
                     EYSDTIKTDVCGVHSCFVTAKDGNNRFMRMPEDILNYRHPRREFIESFLKK"
BASE COUNT           91 a           85 c           67 g           90 t
ORIGIN      
        1 taagccatta tcaaacccac ccgtagatag gctttgtaat ggctacttct tcagaaatga
       61 ttcgatgaat tcacgtcgag gatgacgata gttcagaata tcttctggca tcctcataaa
      121 gcggttgttg ccgtctttgg cagtaacaaa acagctgtga actccgcaga catccgtttt
      181 gatcgtgtcg ctatactcaa aaagcaactg agccatcttc tcttgccatt ctggaggcat
      241 agcctccatg aatactcgcg gcatcacgca gaacgacgca tgcgtaagac caaaccacag
      301 ttgcaggtct ttacgatatt cttcatccat cgt
//
LOCUS       DAZPCS010000513          332 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.523, whole genome shotgun sequence.
ACCESSION   DAZPCS010000513 DAZPCS010000000
VERSION     DAZPCS010000513.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.523"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
BASE COUNT           93 a           60 c           55 g          124 t
ORIGIN      
        1 ccataaacag ggctttaccc gtaaatattg gctccttctg atgccattct atttcaggaa
       61 ggagtgtccg ttaaactcag gctacctcaa aaagttatcg gtgcgtttat cgaaaaacat
      121 atgttctact aattggagtc attgtctgta aaagcaactc aaaaatacaa gttgcttatt
      181 ccccttgttg gtaagaactt aattctaact gtttgttctg ggtgttttaa acgtcctcct
      241 ttttatgctt gttcataacg gatatttaat aggtaacctt tgttttagca tcttctgatt
      301 tatttaaatt atgtgatagt acacacaatt tt
//
LOCUS       DAZPCS010000514          332 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.524, whole genome shotgun sequence.
ACCESSION   DAZPCS010000514 DAZPCS010000000
VERSION     DAZPCS010000514.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.524"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            51..>332
                     /locus_tag="ACHUQ7_004467"
     CDS             51..>332
                     /locus_tag="ACHUQ7_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR1137513.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPD"
BASE COUNT           83 a           70 c           98 g           81 t
ORIGIN      
        1 cgtccgaatg ttagccattc gagggttgtt aagtagtttc aggaggattt atggcgaaac
       61 cttttactcc cgaacagcgg gaagaactga agacgcgaat tgtggaactc gtgcatcagg
      121 acggtcgggt cacgattcgg cagttgtccg atgaaacagg tatcagtcgt gcgtctgtcg
      181 gtcgcttatg catagaactg gtcgcaagtg gtgatgtata taattctggc tacggcttat
      241 tcccgtctga acaggctcgc aaggactggc aaagcgcccg caaaaaactc tcgagagtaa
      301 aggtgaggaa accggttgtt gttgatccgg ac
//
LOCUS       DAZPCS010000515          325 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.525, whole genome shotgun sequence.
ACCESSION   DAZPCS010000515 DAZPCS010000000
VERSION     DAZPCS010000515.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.525"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            103..>325
                     /locus_tag="ACHUQ7_004468"
     CDS             103..>325
                     /locus_tag="ACHUQ7_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HFR1137514.1"
                     /translation="MIYSNQLRYTGKDGVYFHLQGQPDNFLSLSATENMEFDSPMMVT
                     TQNMQSGQTVTDNVMRSPRTISISGVVVVG"
BASE COUNT          110 a           56 c           61 g           98 t
ORIGIN      
        1 aatttacgta gctcacgctt aacagagaaa atacttatat atcaaaggct tggttaaaaa
       61 tgattaaaat gcgctaagtc tttgaaatca aaagagaatt taatgattta ctctaatcag
      121 cttcgttaca ctggcaaaga tggtgtttac ttccatctac aaggccaacc agataatttc
      181 ttgtcactat cagcaactga aaacatggaa tttgatagcc ctatgatggt taccactcaa
      241 aatatgcaat cagggcagac tgtaacagat aatgtaatgc gttcacctag aactatttca
      301 atcagtggtg tggttgtggt tggtg
//
LOCUS       DAZPCS010000516          321 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.526, whole genome shotgun sequence.
ACCESSION   DAZPCS010000516 DAZPCS010000000
VERSION     DAZPCS010000516.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.526"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            26..235
                     /gene="fumD"
                     /locus_tag="ACHUQ7_004469"
     CDS             26..235
                     /gene="fumD"
                     /locus_tag="ACHUQ7_004469"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFR1137515.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT          104 a           57 c           84 g           76 t
ORIGIN      
        1 ttggcatgat aaataggtat aaagaatggg aaatcggaca aaagaggatg aactgtaccg
       61 agaaatgtgc agagttgtcg gtaaagttgt gctggaaatg cgcgatctgg ggcaggaacc
      121 aaagcatatt gttatcgcag gcgtattacg tacagcatta gccaataaac gcattcagcg
      181 cagtgagctg gaaaagcagg cgatggaaac cgtcattaac gcactggtaa aatgaccatt
      241 caggatgagg cggtaagctt catcctgata ttcttgcttc agttgcggtc cagttaatca
      301 ttctaaataa caactgctga a
//
LOCUS       DAZPCS010000517          319 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.527, whole genome shotgun sequence.
ACCESSION   DAZPCS010000517 DAZPCS010000000
VERSION     DAZPCS010000517.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.527"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..>319)
                     /locus_tag="ACHUQ7_004470"
     CDS             complement(<1..>319)
                     /locus_tag="ACHUQ7_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1137516.1"
                     /translation="DNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVGAG
                     LDDVARSLLYAIIVMGILFTGWGIGILFFLLIKKRSKIQDLSFFRLFFAITLLFFPVI
                     FELL"
BASE COUNT          132 a           68 c           44 g           75 t
ORIGIN      
        1 ttaaaagttc gaatattaca gggaaaaaca gtagggtaat agcaaaaaat agtctaaaaa
       61 aactcaagtc ttgtatttta cttcttttct ttataagaag aaaaaataaa ataccaatcc
      121 cccaaccggt aaaaagtata cccattacta tgatagcata gagtaaactt ctggcaacat
      181 catccaaacc tgcaccaaca acccaccatg gaaatccata gtaaaaagag gttccccagc
      241 cataagaata agcactgacc caccctacac atcccatata ggcgatgaat aatgaagaat
      301 ttctaagcaa tgaattgtc
//
LOCUS       DAZPCS010000518          307 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.528, whole genome shotgun sequence.
ACCESSION   DAZPCS010000518 DAZPCS010000000
VERSION     DAZPCS010000518.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.528"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            98..>307
                     /locus_tag="ACHUQ7_004471"
     CDS             98..>307
                     /locus_tag="ACHUQ7_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HFR1137517.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKS"
BASE COUNT           66 a           82 c           89 g           70 t
ORIGIN      
        1 aatttgcgac cattcttgcc ggtatcagcg ccggcggtat gtactgcccg gagtgtggca
       61 cggttcactg gccggacgga gtcgccccgc ccttctgatg ctcccccgtt ttgccgatat
      121 ttttcagcag ggaaaccgct ggcttaactg gctggagaaa caaccggaag gggcagtccg
      181 tccggtggta attgaatcag tgacaaaaat catggcctgc gggaccacac tgatgggcta
      241 cacgcagtgg tgttgttcat caccggactg ttgccataca aaaaaggtct gcttccggtg
      301 taaaagc
//
LOCUS       DAZPCS010000519          295 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.530, whole genome shotgun sequence.
ACCESSION   DAZPCS010000519 DAZPCS010000000
VERSION     DAZPCS010000519.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.530"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
BASE COUNT           95 a           55 c           65 g           80 t
ORIGIN      
        1 tcggttagtt aaacatcgta cttcatattt gaacgttctg ccggaatgca ttatcaatag
       61 aggtaaagtc gcaaccccaa atcgtaaagg aaaccgtagc acgtcgtatg caagaacgtg
      121 ccacggctgg ctgatggatg ttcgatagcg cgagtttgaa tgaaaatcag ccggagatga
      181 ttttacataa ttgctacgga attattcaat acaggaattg cttgcgtctg catggattga
      241 cctgaaatat tcccgaaaat ttctctaaaa aactcgaaaa aaatggtaac tggtt
//
LOCUS       DAZPCS010000520          293 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.denovo.531, whole genome shotgun sequence.
ACCESSION   DAZPCS010000520 DAZPCS010000000
VERSION     DAZPCS010000520.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.denovo.531"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(<1..>293)
                     /locus_tag="ACHUQ7_004472"
     CDS             complement(<1..>293)
                     /locus_tag="ACHUQ7_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFR1137518.1"
                     /translation="KALPQSNIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLN
                     ELIALEFKDEYGAVVIDVDAMQSKSAERLCNEDFNGSYFNSGVMYINLREWLK"
BASE COUNT          109 a           66 c           35 g           83 t
ORIGIN      
        1 ttttaaccat tcccgtaaat taatatacat tacaccagag ttaaaatagc taccgttaaa
       61 atcctcatta cacaaacgct cagcgctttt actttgcata gcatctacat caattaccac
      121 tgccccatat tcatctttaa actctaatgc tattaactcg ttcaatgaac ccttacagac
      181 gatatcagca tcaagatata ataatgaatc caatcgcgca gaaaaataat caaatgaaag
      241 caaacgataa taaatagata ctggccagat atttgattga ggtagcgcct tca
//
LOCUS       DAZPCS010000521         9712 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.guided.140, whole genome shotgun sequence.
ACCESSION   DAZPCS010000521 DAZPCS010000000
VERSION     DAZPCS010000521.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9712
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.guided.140"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            complement(35..205)
                     /locus_tag="ACHUQ7_004473"
                     /pseudo
     CDS             complement(35..205)
                     /locus_tag="ACHUQ7_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(424..1401)
                     /gene="epmA"
                     /locus_tag="ACHUQ7_004474"
     CDS             complement(424..1401)
                     /gene="epmA"
                     /locus_tag="ACHUQ7_004474"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFR1137519.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1726..3534
                     /gene="frdA"
                     /locus_tag="ACHUQ7_004475"
     CDS             1726..3534
                     /gene="frdA"
                     /locus_tag="ACHUQ7_004475"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFR1137520.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            3527..4261
                     /gene="frdB"
                     /locus_tag="ACHUQ7_004476"
     CDS             3527..4261
                     /gene="frdB"
                     /locus_tag="ACHUQ7_004476"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFR1137521.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4272..4667
                     /gene="frdC"
                     /locus_tag="ACHUQ7_004477"
     CDS             4272..4667
                     /gene="frdC"
                     /locus_tag="ACHUQ7_004477"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFR1137522.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FALFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4678..5037
                     /gene="frdD"
                     /locus_tag="ACHUQ7_004478"
     CDS             4678..5037
                     /gene="frdD"
                     /locus_tag="ACHUQ7_004478"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFR1137523.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            5100..6232
                     /locus_tag="ACHUQ7_004479"
                     /pseudo
     CDS             5100..6232
                     /locus_tag="ACHUQ7_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
     gene            6321..6854
                     /gene="blc"
                     /locus_tag="ACHUQ7_004480"
     CDS             6321..6854
                     /gene="blc"
                     /locus_tag="ACHUQ7_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFR1137524.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6851..7168)
                     /gene="sugE"
                     /locus_tag="ACHUQ7_004481"
     CDS             complement(6851..7168)
                     /gene="sugE"
                     /locus_tag="ACHUQ7_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFR1137525.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7344..7490)
                     /gene="ecnB"
                     /locus_tag="ACHUQ7_004482"
     CDS             complement(7344..7490)
                     /gene="ecnB"
                     /locus_tag="ACHUQ7_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFR1137526.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7601..7726)
                     /gene="ecnA"
                     /locus_tag="ACHUQ7_004483"
     CDS             complement(7601..7726)
                     /gene="ecnA"
                     /locus_tag="ACHUQ7_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFR1137527.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(7778..8344)
                     /gene="efp"
                     /locus_tag="ACHUQ7_004484"
     CDS             complement(7778..8344)
                     /gene="efp"
                     /locus_tag="ACHUQ7_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFR1137528.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8386..9414
                     /gene="epmB"
                     /locus_tag="ACHUQ7_004485"
     CDS             8386..9414
                     /gene="epmB"
                     /locus_tag="ACHUQ7_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFR1137529.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2472 a         2484 c         2471 g         2285 t
ORIGIN      
        1 tgaacataaa acactatcag taagttggag tcattaccat taaggcgaat ggaataaaga
       61 ataacaatgc agaaaatata taaaagggaa tcgcactata acccattaag taataagccg
      121 atgggctatt ggcaaatcca aatacggaag taaagatcat caatatgagt cctatcagac
      181 tcatcttttt tatcgtgtga ggcatgaaac catccttcat ttcagcgcgt gtataacacg
      241 tgcccactga actgcactgc gcaacattga gcaggcagcg taaaaaccaa tacagaattg
      301 atacttggat gatatcaaat atccagtctt aaattgtggc ctttaacgga aaaatgaaaa
      361 catatgctgc agaatggcgc ttatcacgcc attcttcgct gttaattcag taatttttca
      421 gaattatgcc cggtcaacgc taaaggcgat gacttcagcc agtgtctccg cgcccagcgc
      481 caacatcacc agacgatcaa cacctaatgc cacgccggaa cagtcaggca taccgacttt
      541 caaggcttca atcagattct ggtcaatggg gtgctgcggc aaaccgcgcg ccgcgcgctt
      601 acggttatct tgttcaaagc gttgttgctg ctcacgggca tccgttaatt catggaaacc
      661 attcgccagc tcaatacctt tataataaac ctcaaagcgt tcagcgaccc gatgatcttc
      721 ggtactgatt tgcgccagtg atgcctggct ggctggaaag tggtacacaa aggtcggttt
      781 ttctttacca atatttggct ctacgccaaa ggtaaacagc aattgtagca acgtgtcgcg
      841 gtcttcttcg gtatcagcaa cattgctcaa atccagtttc gctgcgactt cccgcagttg
      901 cgttttgtcg gcagagagcg ggtcaatttc cagataacgc aagaaagctt gttgataaga
      961 aaggctttct gctgccgggc agtccagcac ctgttgtaag agatcgtcca cctcgttcat
     1021 caaccggtac atatcatagt gcggtcgata ccactccagc atagtgaact cagggttgtg
     1081 ataacgcccc atctcttcat tacggaagct gcggcacagc tggaataccg gcccacaacc
     1141 agcaaccagc aggcgtttca tatggtattc cgggctggtc attaaccaga gattcatccc
     1201 ctgcgaatgc ccggggccaa cgaaacgtgt ctcaaacggg accaaatgaa tatcggttac
     1261 cgtcgcctgg ctcatacaag gcgtctccac ctccagcact ccacgatcgg caaagaaacg
     1321 acggatctcc gccataatcg ccgcgcgttt taataagtta ggaatggatg cgctcggctg
     1381 ccaggatgcc gtttcgctca tagttaaatc tccagttttt gacaagggca cgaagtctac
     1441 tcgcaacgcg acggcgagac aaattttacg caggaatcaa acagcggtgg gcagtgacta
     1501 aaaaaagcac gatctgatgg tttagtaatt aaattaatca tcttcagtga taatttagcc
     1561 ctcttgcgca ctaaaaaaat cgatctcgtc aaatttcaga cttatccatc agactatact
     1621 gttgtaccta taaaggagca gtggaatagc gttcgcagac cgtaactttc aggcacttac
     1681 cctgaagtac ggggctgtgg gataaaaaca atctggagga atgtcgtgca aacctttcaa
     1741 gccgatcttg ccattgtagg cgccggtggc gcgggattac gtgctgcaat tgctgccgcg
     1801 caggcaaatc caaatgcaaa aatcgcacta atctcaaaag tatacccgat gcgtagccat
     1861 accgttgctg cagaaggggg ctccgccgct gtcgcgcagg atcatgacag cttcgaatat
     1921 cactttcacg atacagtagc gggtggcgac tggttgtgtg agcaggatgt cgtggattat
     1981 ttcgtccacc actgcccaac cgaaatgacc caactggaac tgtggggatg cccatggagc
     2041 cgtcgcccgg atggtagcgt caacgtacgt cgcttcggcg gcatgaaaat cgagcgcacc
     2101 tggttcgccg ccgataagac cggcttccat atgctgcaca cgctgttcca gacctctctg
     2161 caattcccgc agatccagcg ttttgacgaa catttcgtgc tggatattct ggttgatgat
     2221 ggtcatgttc gcggcctggt agcaataaac atgatggaag gcacgttggt gcagatccgt
     2281 gctaacgcgg tcgttatggc taccggcggt gcgggtcgcg tttatcgtta caacaccaac
     2341 ggcggcatcg ttaccggtga cggtatgggt atggcgctaa gccacggcgt tccgctgcgt
     2401 gacatggaat tcgttcagta tcacccaacc ggtctgccag gttccggtat cctgatgacc
     2461 gaaggctgcc gtggtgaagg cggtattctg gtcaacaaaa atggctaccg ttatctgcaa
     2521 gattacggca tgggcccgga aactccgctg ggcgagccga aaaacaaata tatggaactg
     2581 ggtccacgcg acaaagtttc tcaggccttc tggcacgaat ggcgtaaagg caacaccatc
     2641 tccacgccgc gtggtgatgt ggtttatctc gacctgcgtc acctcggcga gaaaaaactg
     2701 catgaacgtc tgccgttcat ctgcgaactg gcgaaagcgt acgttggcgt cgatccggtt
     2761 aaagaaccga ttccggtacg tccgactgca cactacacca tgggcggtat cgaaaccgat
     2821 cagaactgtg aaacccgcat taaaggtctg ttcgccgtgg gtgaatgttc ctctgttggt
     2881 ctgcacggtg caaaccgtct gggctccaac tccctggcgg aactggtggt cttcggccgt
     2941 ctggccggtg aacaagcgac agagcgtgca gcaactgccg gtaatggcaa cgaagcggca
     3001 attgaagcgc aggcagctgg cgttgaacaa cgtctgaaag atctagttaa ccaggatggc
     3061 ggcgaaaact gggcgaagat ccgcgacgaa atgggcctgg caatggaaga aggttgcggt
     3121 atctaccgta cgccggaact gatgcagaaa accatcgaca agctggcaga gctgcaggaa
     3181 cgcttcaagc gcgtgcgcat caccgacacc tccagcgtgt tcaacaccga cctgctctac
     3241 accattgaac tgagccacgg tctgaacgtt gctgaatgta tggcgcactc cgcaatggca
     3301 cgtaaagagt cccgcggcgc acaccagcgt ctggacgaag gttgcaccga gcgtgacgac
     3361 gtcaactttc tcaaacacac cctcgccttc cgcgatgctg atggcacgac tcgcctggag
     3421 tacagcgacg tgaagattac tacgctgccg ccagctaaac gcgtttacgg tggcgaagcg
     3481 gatgcagccg ataaggcgga agcagccaat aataaggaga aggcgaatgg ctgagatgaa
     3541 aaacctgaaa attgaggtgg tgcgctataa cccggaagtc gataccgcac cgcatagcgc
     3601 attctatgaa gtgccttatg acgcaactac ctcattactg gatgcgctgg gctacatcaa
     3661 agacaacctg gcaccggacc tgagttaccg ctggtcctgc cgtatggcga tttgtggttc
     3721 ctgcggcatg atggttaaca acgtgccaaa actggcatgt aaaaccttcc tgcgtgatta
     3781 caccgacggt atgaaggttg aagcgttagc taacttcccg attgaacgcg atctggtggt
     3841 cgatatgacc cacttcatcg aaagtctgga agcgatcaaa ccgtacatca tcggcaactc
     3901 ccgcaccgcg gatcagggta ctaacatcca gaccccggcg cagatggcaa agtatcacca
     3961 gttctccggt tgcatcaact gtggtctgtg ctacgccgcg tgcccgcagt ttggcctgaa
     4021 cccagagttc atcggtccgg ctgccattac gctggcgcat cgttataacg aagatagccg
     4081 cgaccacggt aagaaggagc gtatggcgca gttgaacagc cagaacggtg tatggagctg
     4141 tactttcgtg ggctattgct ccgaagtctg cccgaaacac gtcgatccgg ctgcggccat
     4201 tcagcagggc aaagtagaaa gttcgaaaga ctttcttatc gcgaccctga aaccacgcta
     4261 aggagtgcaa catgacgact aaacgtaaac cgtatgtacg gccaatgacg tccacctggt
     4321 ggaaaaaatt gccgttttat cgcttttaca tgctgcgcga aggcacggcg gttccggctg
     4381 tgtggttcag cattgaactg attttcgcgc tgtttgccct gaaaaatggc ccggaagcct
     4441 gggcgggatt cgtcgacttt ttacaaaacc cggttatcgt gatcattaac ctgatcactc
     4501 tgacggcagc tctgctgcac accaaaacct ggtttgaact ggcaccgaaa gcggccaata
     4561 tcattgtaaa agacgaaaaa atgggaccag agccaattat caaaagtctc tgggcggtaa
     4621 ctgtggttgc caccatcgta atcctgtttg ttgccctgta ctggtaagga gcctgagatg
     4681 attaatccaa atccaaagcg ttctgacgaa ccggtattct ggggcctctt cggggccggt
     4741 ggtatgtgga gcgcaattat tgcgccggtg atgatcctgc tggtgggtat tctgctgcca
     4801 ctggggctgt ttccgggtga tgcgctgagc tacgagcgcg ttctggcgtt cgcgcagagc
     4861 ttcattggtc gcgtattcct gttcctgatg atcgttctgc cgctgtggtg tggtttacac
     4921 cgtatgcacc acgcgatgca cgatctgaaa atccacgtgc ctgcgggcaa atgggttttc
     4981 tacggtctgg ctgctatcct gacagttgtc acgctgattg gtgtcgttac aatctaacgc
     5041 atcgccaatg taaatccggc ccgcctatgg cgggccgttt tgtatggaaa ccagacctta
     5101 tgttcaaaac gacgctctgc gccttattaa ttaccgcctc ttactccaca tttgctgccc
     5161 ctcaacaaat caacgatatt gtgcatcgca caattacccc gcttatagag caacaaaaga
     5221 tccccggtat ggcggtggcg gtaatttatc agggtaaacc ttattacttt acctggggct
     5281 atgcggacat cgccaaaaag cagcccgtca cacagcaaac gttgtttgag ttaggttcgg
     5341 tcagcaaaac atttacgggc gtgcttggtg gcgacgctat tgctcgaggg gaaatcaagt
     5401 taagcgatcc cgcaacaaaa tactggcctg aacttaccgc taaacagtgg aatgggatca
     5461 cactattaca tcttgcgacc tacaccgctg gcggcctgcc attgcaggtg ccggatgaag
     5521 tgaaatcctc aagcgacttg ctgcgcttct atcaaaactg gcagcctgca tgggctccag
     5581 gaacacaacg tctgtatgcc aactccagta tcggtttgtt cggcgcactg gctgtgaagc
     5641 cgtctggttt gagttttgag caggcgatgc aaactcgtgt cttccagcca ctcaaactca
     5701 accatacgtg gattaatgta cctcccccag aagaaaagaa ttacgcctgg ggatatcgcg
     5761 aaggtaaggc agtgcatgtt tcgccagggg cgttagatgc tgaagcttat ggtgtgaagt
     5821 cgaccattga agatatggcc cgctgggtgc aaagcaattt accccccttg atatcaatga
     5881 gaaaacgctt caacaaggga tacaactggc acaatctcgc tactggcaaa ccggcgatat
     5941 gtatcagggc ctgggctggg aaatgctgga ctggccggtc aatcctgaca gcatcattaa
     6001 cggcagtgac aataaaattg cactggcagc acgcccggta aaagcgatta agcccccaac
     6061 tcctgcagta cgcgcatcat gggtacataa aacgggggcg accggcggat ttggtagcta
     6121 tgtcgcgttt attccagaaa aagagctggg tatcgtgatg ctggctaaca aaaactatcc
     6181 caatccagcg agagtcgccg ccgcctgtca gattctcaac gctctacagt aaaatttcat
     6241 cgggtccgaa ttttcggacc ttttctgcgc ttttccttgc tgtcatctac acttagaaaa
     6301 aaaccagtaa ggaaacattt atgcgcctgc tccctctcgt tgccgcagcg acagctgcat
     6361 ttctggtcgt tgcctgtagt tttcctacgc cgccgcgtgg cgtgaccgta gtgaataatt
     6421 tcgacgccaa acgttatctc ggtacctggt atgagattgc ccgttttgat caccgttttg
     6481 aacgtggact ggaaaaagtc accgcaacat acagcctgcg tgatgacggc ggcctgaatg
     6541 tcattaataa aggctataac cctgacagag gaatgtggca gcagagtgaa gggaaagcgt
     6601 actttaccgg cgcaccaact cgcgctgcgc tgaaagtgtc attctttggt cctttctatg
     6661 gcggttataa cgttattgca ctcgatcggg aataccgcca tgcgctggtt tgcggcccgg
     6721 accgcgacta cctgtggata ctctcccgca cgccaaccat ttctgacgaa gtgaaacagg
     6781 agatgctggc agtcgcgacc cgggaagggt ttgatgtcag taagtttatt tgggtacagc
     6841 agcccggtag ttagtgagtg ctgagtttca gaccaataat ccccaatacg attaacgcca
     6901 gactcgccag acgcatcggg ttagcggact caccgagcag cacaatgccg gtgatggccg
     6961 cgccgactgc gccaataccc gtccacacgg cataagccgt ccctaccggt aacgatttca
     7021 tcgcccaggc aagtagcgcc atactgacta tcatcgccgt cacggtaata acactcggcg
     7081 tcaaacggct aaagccgtgg gtatatttca ggccaacggc ccataccact tccagcagac
     7141 cagcaataac taagataatc caggacatat caggctccgg aaatttgggg ccgtccccgg
     7201 tgaaaagagg cgtttgcagg tcgtcctgca aagctgatgg ttgaacggat attttgccag
     7261 gtggcagggg aatttcaact attaccttta tgacgatcgc agaaaaaaca aacggcacga
     7321 cactgctgtc gtaccgttat tgcttattgc tgcgctttcg ttgctgcgcc agaaatcgcg
     7381 ttaccgccat cagaaatgtc ttcaccaacg ccacgcgtgg tgttgcaggc agttaatact
     7441 gttgaaagca ccagaacaga aaagatcgct gcaattgtct tcttcaccat aacgtcttcc
     7501 ttttaggtaa tgttatttat gtttgcctat agcaaattaa taatagacaa catcgccaaa
     7561 aatgatggaa atctgatgat ttttcggaag agaagaaaaa ttagctggca gcgcgagaga
     7621 tggagttacc gagatgtttg atgtcttcgc cgaaaccgca agcggtgtta cagcccgtga
     7681 gcagcgtgct ggcaagcaaa acaagaacga taaggcgttt catcatctct gacctgaata
     7741 agtgatggtg cagcctgcag gccgcaccac aaccgcatta cttcacgcga gagacgtatt
     7801 caccagagcg ggtatccact ttgatgactt cgccgatttg tacgaacagc ggaactttaa
     7861 ccacagcgcc agtagacagg gtagccggtt tgccgccagt acctgcggta tcacctttca
     7921 ggcccggatc ggtatcaacg atttccagtt caacgaagtt cggcggagta acggagatcg
     7981 gctgaccatt ccacagagtt acgatacact ctgcctgatc cagcagccat ttagcattgt
     8041 cgccaattgc ttttgcatca gcagacagct gctcgaaagt ttcgttgttc atgaagtgcc
     8101 agaactcacc gtcgttgtac aggtaagtca ggttcatatc gacaacatca gcgccttcag
     8161 cggaatcagt agatttgaag gttttttcta cgcgagtacc ggtcagcaga cgacgcagtt
     8221 taacgcgagc aaatgcctgg cctttacccg gttttacgaa ttcactcgct tcaaccgcgt
     8281 aaggttcgcc gtctaacatg attttaagac cagcacgaaa atcgttgcta tagtacgttg
     8341 ccataaggcc ctctgaaatt tgttaattgg tagctaagcc acaaaatggc gcatattgta
     8401 accctaaata ccccatccag agaagattgg ttaacgcaac ttgccgatgt tgtgaccgat
     8461 cctgatgaac ttctgcgtct tttgaatata gacgcggacg aaaaactgtt ggccggacgc
     8521 agcgccaaaa agctgtttgc cctgcgtgtg ccccgctcat ttatcgatcg catggagaaa
     8581 ggcaatccgg acgatcctct tttgcgtcag gtacttacct cgcaagatga gtttgtcgtc
     8641 gcgtccggat tctccaccga cccactggaa gaacagcaca gcgtagtgcc tggtttgttg
     8701 cataaatacc acaaccgggc gcttttgctg gtcaaaggcg gctgcgcggt aaattgccgc
     8761 tattgcttcc gtcgccactt cccctatgcc gaaaatcagg gcaacaagcg taactggcag
     8821 actgcacttg agtatgttgc tgcgcatccg gaactggacg agatgatttt ctccggcggc
     8881 gatccgctga tggcgaaaga gcacgagctg gactggttgc tcacacaact ggaagccatc
     8941 ccgcatatca aacgtctgcg gattcacagc cgtctgccga ttgtgatccc ggcacgtatc
     9001 accgaggcgc tggttgaacg ctttgcccgt tctacgctgc aaatcttgct ggtgaatcac
     9061 atcaatcatg ccaatgaggt agatgaaacg ttccgtcagg cgatggctaa attgcgccgt
     9121 gtcggtgtca ccctgcttaa ccagagcgtt ctgttacgtg gtgtgaacga taacgcacaa
     9181 acgttggcaa acctgagtaa tgcgttgttc gatgccggcg tgatgccgta ttacctgcat
     9241 gtgctagata aagtacaggg cgcggcgcat tttatggtga gtgatgacga agcacggcag
     9301 attatgcgtg agttgctgac attggtgtcg ggatatctgg tgccgaaact ggcgcgagaa
     9361 attggcggcg aacccagcaa aacgccgctg gatctccagc tacgccagca gtaactgatt
     9421 aaacagggaa gttaaacatt aaaattgcct gatgcgctac gcttatcagg cctacatcgt
     9481 gcattgaaat tgaatggatt tgcaatgaac tgtaggccgg ataaggcgtt tacgccgcat
     9541 ccggcaagag caacaaacgc aagtacagca accactgaga aatttaatct ttgcacgttg
     9601 cataaccttt aattattcat atgcattgct gttgttactt tttatatagc taacaccaat
     9661 aattaacctt tcttttcggt aatgactcca acttactgat agtgttttat gt
//
LOCUS       DAZPCS010000522         7991 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.guided.161, whole genome shotgun sequence.
ACCESSION   DAZPCS010000522 DAZPCS010000000
VERSION     DAZPCS010000522.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7991
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.guided.161"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            263..922
                     /locus_tag="ACHUQ7_004486"
     CDS             263..922
                     /locus_tag="ACHUQ7_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HFR1137530.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     gene            complement(1123..1500)
                     /locus_tag="ACHUQ7_004487"
     CDS             complement(1123..1500)
                     /locus_tag="ACHUQ7_004487"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR1137531.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(1567..4533)
                     /locus_tag="ACHUQ7_004488"
     CDS             complement(1567..4533)
                     /locus_tag="ACHUQ7_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HFR1137532.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            complement(4536..5096)
                     /locus_tag="ACHUQ7_004489"
     CDS             complement(4536..5096)
                     /locus_tag="ACHUQ7_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR1137533.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            complement(5222..5572)
                     /locus_tag="ACHUQ7_004490"
                     /pseudo
     CDS             complement(5222..5572)
                     /locus_tag="ACHUQ7_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636771.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
     gene            complement(5775..6788)
                     /gene="intI1"
                     /locus_tag="ACHUQ7_004491"
     CDS             complement(5775..6788)
                     /gene="intI1"
                     /locus_tag="ACHUQ7_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HFR1137534.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            6998..7828
                     /locus_tag="ACHUQ7_004492"
     CDS             6998..7828
                     /locus_tag="ACHUQ7_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HFR1137535.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
BASE COUNT         1807 a         2154 c         2258 g         1772 t
ORIGIN      
        1 caccatcata cactaaatca gtaagttggc agcatcaccc gacgcacttt gcgccgaata
       61 aatacctgtg acggaagatc acttcgcaga ataaataaat cctggtgtcc ctgttgatac
      121 cgggaagccc tgggccaact tttggcgaaa atgagacgtt gatcggcacg taagaggttc
      181 caactttcac cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt
      241 ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat
      301 atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta
      361 taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca
      421 caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt
      481 ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac
      541 cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt
      601 ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta
      661 tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt
      721 caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat
      781 gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca
      841 tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga
      901 tgagtggcag ggcggggcgt aattttttta aggcagttat tggtgccctt aaacgcctgg
      961 ttgctacgcc tgaataagtg ataataagcg gatgaatggc agaaattcga aagcaaattc
     1021 gacccggtcg tcggttcagg gcagggtcgt taaatagccg cttatgtcta ttgctggttt
     1081 accggtttat tgactaccgg aagcagtgtg accgtgtgct tctcaaatgc ctgaggccag
     1141 tttgctcagg ctctccccgt ggaggtaata attgacgata tgatcattta ttctgcctcc
     1201 cagagcctga taaaaacggt tagcgccggg gttggatttt tcagcgttcc agctaaggct
     1261 aaggcattcc tgttcaagcg caagccgggc tataaagcgc attatcgctt tacccgtgcc
     1321 cttatttcga tcgcactgag aaacatacag ctctttaata tgcagctgac cgctgtatcg
     1381 gggcgaggga taaagaatat tgcaacatgc caggccagta atgttattgc cgcagcgcgc
     1441 tctgatcacc agggtaccgg aaagccggtt gaataacttt ttacagagat aatccttcat
     1501 caacgcttcc tgaatgatac cttcgccata ataggggtcg tctcagaaaa cggaaaataa
     1561 agcacgctaa gccggttgca gaggccgtag cggcctgaac ttccccgcgc cgatcttggc
     1621 gctgctgcgc cataggtaat caccggtcag gttgatgtgc tcccagccga gtggcgacag
     1681 gtactgcaat agcgagtcat cgacggcatg accattgccg cgcaacgcat gcgccgcacg
     1741 ctccaggtag accgtgttcc acagcacgat ggccgccgtc accaggttga ggccgctggc
     1801 ccggtagcgc tgctgctcga aactgcggtc acggatttca ccaaggcggt tgaagaacac
     1861 ggcacgggcc agcgcattgc gcgcctcgcc cttgttcagc ccggcatgca cgcggcggcg
     1921 tagctcgacg ctttgcagcc agtcgaggat gaacagcgtg cgctcgatgc ggcccaactc
     1981 gcgcagcgcg acggccaagc cgttctggcg cgggtagctg ccgagtttcc tgagcatcag
     2041 cgaggccgtc accgtgccct gcttgatcga ggtggccagc cgcaggattt cgtcccaatg
     2101 ggcgcggacg tgcttgatgt tgagcgtgcc gccgatcatc ggcttgagcg cgtcataggc
     2161 ggcatcgccc ttcgggatgt agagcttggt gtcgcccagg tcgcggatgc gcggcgcgaa
     2221 gcggaagccc aagaggtgca tcagggcgaa gacgtgatcg gtgaagcccg ccgtgtcggt
     2281 gtagtgctcc tcgatccgca ggtcggattc gtggtacagc aggccgtcga gcacgtaggt
     2341 tgagtcgcgc aggccgacat tgaccacctt ggtgtggaat ggcgcgtatt ggtcggagat
     2401 gtgggtgtag aaagtccgtc ctgggctgct gccatatttt gggttgatgt gccccgtgct
     2461 ctttgccttg ctagcggttc ggaaattctg tccgtccgat gatgatgtgg tgccatcgcc
     2521 ccagtgcccg gcaaagggat gccgaaactg agcgttgacc agttcagcca acgctgtcga
     2581 gtacgtttcg tcgcgggtat gccaggcttg cagccaagcg agcttcgcgt aggtcgtgcc
     2641 ggggcaggac tcggccatct tggtcaggcc caggttgatc gcgtcggcca ggatcgtggt
     2701 caacaacagg ttcttgtcct tggccagatc gcccgatttc aagtgcgtga agtgccgggt
     2761 gaagcccgtc cactcatcga cttcgagcag cagttcggtg atcttgacgt gcggcaggac
     2821 catggctgtc tggtctatca gcgcctgcgc ggtgtcgggc accgccgcat ccagcggcgt
     2881 gatcttcaag cccgactcgg tgatgatggc atccggcagg tcgttggctg ccgccatgcg
     2941 gttgacggtg gcaagttgtg cttccagcag cgtcagccgc tcatgcagat attgttcgca
     3001 gtcggtggcc acggccagcg gcaattcgct ggactgcttg aggctggtga acttctcggg
     3061 cggtaccagg tagtcctcga agtccttgaa ctggcgtgaa ccctgcaccc agatgtcgcc
     3121 cgagcgcagg gagttcttca actcggacag cgcgcacagt tcgtagtagc gccggtcgat
     3181 gccggcgtcg gtcatcacca gtttctgcca gcgcggcttg atgaagccgg tcggtgcatc
     3241 ggctggcagc ttgcgggcgt tgtcggtgtt catgccgcgc agcacctcaa tggcatcaag
     3301 cacgtttttg gcggcgggcg cggcccgcag cttgagcacg gcaaggaatt ccggtgcata
     3361 gcggcgcagg gtggcgtagc tctcgccgat gcgatgcagg aaatcgaagt catcgggttg
     3421 cgcgagcttc tgcgcctcgg tgacgctctc ggcaaaggaa tcccaggaca tgacggcctc
     3481 gatggcggca aacgcatcgc ggcctgattg cttggcgtcg atcagcgcct gaccgatgcg
     3541 cccgtacaga cgtaccttgg cgttgatggc cttgcctgac gcctggaact gctgctgatg
     3601 cttattcttg gcagcgttaa acagcttacc caggatgcgg tcgtgcaggt cgatgatttc
     3661 gtcggtgacg gtggccatgc cctcggtggc cagcgccacg agagtggcgt agcgccgttg
     3721 cggctcgaat ttggccaggt cggcgggtgt catctggccg ccctcgcggg caatcttgag
     3781 caggcggttc tggtgaacca gccgctcgat gccggtaggc agatcgagtg cctgccatgc
     3841 cttgaggcgt tcgatgtgtt ccagcatatg ccgcgaattt ggcttggccg gagactggcg
     3901 caaccaagcc aaccaggtcg tcttgccgtt gtcccggcgc ttgagcagat cgtcgaggcg
     3961 gcggcgatgc gcgtccgcca gtggttcggc caaggcgtcg tagatgcgcc ggttagcacg
     4021 ggtgatcgcc tcggcactcg cccgctcgac ggcgttgagg gcgggcagaa tgaccgactg
     4081 ccgccgcagg tgcccgatca aggcgctggc cagcacgatg cctttgtcgg tttgcatcgc
     4141 cagctcggtc agcatctgga cggcctgccg gtaatggctc atggtgaagg gccggaaacc
     4201 gaacacggtt tgcagctcgc tcaggtgctc gcgccgggtc tgctcccgct ggccgtactc
     4261 gttccagctt tcgacgccga ccttgagctg gtcggcgacc agcttcaaca agggcgggaa
     4321 cggtagttca tcgacgccca ggatgacgcc gggaaagcgc aggtaacaga gctgcaccgc
     4381 gaagcccagc cgattggctg gcccgcgccg ctgtcggatg atcgagaggt cggtatcgtt
     4441 gaatgtgtaa tgtcggatca ggtcgtcctt ggagtccggc aacgccagca ggctttcccg
     4501 ctcggcggcg gacaggatgg aacgacgtgg catatttact gatccgttct caagtattga
     4561 tacagggttt cgcgactgat tccgaattca cgagcaagct tggtcttttg ctcgccagcc
     4621 tcgacacgtt ggcgcagttc ggcaatacgc tcagacgaca gggatttctt cctgccacgg
     4681 taagccccgc gttgcttggc gagcgcaata ccctcgcgct gacgctcgcg gatcagggcg
     4741 cgctcgaact cggcgaacgc gcccatcacc gagagcatca ggttcgccat cggagagtct
     4801 tcgccagtaa aactgaggtg ttccttgacg aattcgatat gcacgccgcg ttgtgtcagc
     4861 gtttgcacga tccggcgcaa atcatcgaga ttgcgcgcca ggcgatccat gctatgcacc
     4921 accacggtgt cgccggtgcg ggcgaagctt atcagcgctt ccagttgcgg acgcttgaca
     4981 tccttgccgg atgccttgtc gctaaaagcg cgatcaacct tgacgccttc cagttgccgt
     5041 tccgggttct ggtcgaaggt gctgaccctg atatacccaa tgcgctgtcc agtcatggaa
     5101 ttccctgcaa aatgtcaggg aagactctat gaccttcaac gagatatgtc aataaattca
     5161 aaattcaatc ctatcctgac gcaatttaca catggcatct gacatcaggt tagggtatgc
     5221 ctcaacctga cggcggcgaa tcacaagcgt ccggtttgac gctggtttcg gtcgcagtgc
     5281 tggcccgcgc ctggaagcgc tgatagcact ccagcccgca gaaatgctcc acgtactcgg
     5341 ccccttccgg cgtgaaggcg gcatcgagcg ggatttcctt gcagcacacg cagcaactgg
     5401 tgctcggttc attggcgttc atggtggtgt tcctccattg gttgacgaag ccgacgaagg
     5461 ccgccgccgg catcggcctg gcgaacagga aaccctgcac cgtgtcgcaa cccgcctgcc
     5521 gcaaccacgc aaggcagtcg ggtgtttcca caccctcggc taccacctcc attgtcgttt
     5581 tcagaagacg gctgcactga acgtcagaag ccgactgcac tatagcagcg gaggggttgg
     5641 atccatcagg caacgacggg ctgctgccgg ccatcagcgg acgcagggag gactttccgc
     5701 aaccggccgt tcgatgcggc accgatggcc ttcgcgcagg ggtagtgaat ccgccaggat
     5761 tgacttgcgc tgccctacct ctcactagtg aggggcggca gcgcatcaag cggtgagcgc
     5821 actccggcac cgccaacttt cagcacatgc gtgtaaatca tcgtcgtaga gacgtcggaa
     5881 tggccgagca gatcctgcac ggttcgaatg tcgtaaccgc tgcggagcaa ggccgtcgcg
     5941 aacgagtggc ggagggtgtg cggtgtggcg ggcttcgtga tgcctgcttg ttctacggca
     6001 cgtttgaagg cgcgctgaaa ggtctggtca tacatgtgat ggcgacgcac gacaccgctc
     6061 cgtggatcgg tcgaatgcgt gtgctgcgca aaaacccaga accacggcca ggaatgcccg
     6121 gcgcgcggat acttccgctc aagggcgtcg ggaagcgcaa cgccgctgcg gccctcggcc
     6181 tggtccttca gccaccatgc ccgtgcacgc gacagctgct cgcgcaggct gggtgccaag
     6241 ctctcgggta acatcaaggc ccgatccttg gagcccttgc cctcccgcac gatgatcgtg
     6301 ccgtgatcga aatccagatc cttgacccgc agttgcaaac cctcactgat ccgcatgccc
     6361 gttccataca gaagctgggc gaacaaacga tgctcgcctt ccagaaaacc gaggatgcga
     6421 accacttcat ccggggtcag caccaccggc aagcgccgcg acggccgagg tcttccgatc
     6481 tcctgaagcc agggcagatc cgtgcacagc accttgccgt agaagaacag caaggccgcc
     6541 aatgcctgac gatgcgtgga gaccgaaacc ttgcgctcgt tcgccagcca ggacagaaat
     6601 gcctcgactt cgctgctgcc caaggttgcc gggtgacgca caccgtggaa acggatgaag
     6661 gcacgaaccc agtggacata agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg
     6721 cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg
     6781 gttttcatgg cttgttatga ctgttttttt ggggtacagt ctatgcctcg ggcatccaag
     6841 cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg
     6901 cagcagggca gtcgccctaa aacaaagttg ggcgaacccg gagcctcatt aattgttagc
     6961 cgttaaaatt aagcccttta ccaaaccaat acttattatg aaaaacacaa tacatatcaa
     7021 cttcgctatt tttttaataa ttgcaaatat tatctacagc agcgccagtg catcaacaga
     7081 tatctctact gttgcatctc cattatttga aggaactgaa ggttgttttt tactttacga
     7141 tgcatccaca aacgctgaaa ttgctcaatt caataaagca aagtgtgcaa cgcaaatggc
     7201 accagattca actttcaaga tcgcattatc acttatggca tttgatgcgg aaataataga
     7261 tcagaaaacc atattcaaat gggataaaac ccccaaagga atggagatct ggaacagcaa
     7321 tcatacacca aagacgtgga tgcaattttc tgttgtttgg gtttcgcaag aaataaccca
     7381 aaaaattgga ttaaataaaa tcaagaatta tctcaaagat tttgattatg gaaatcaaga
     7441 cttctctgga gataaagaaa gaaacaacgg attaacagaa gcatggctcg aaagtagctt
     7501 aaaaatttca ccagaagaac aaattcaatt cctgcgtaaa attattaatc acaatctccc
     7561 agttaaaaac tcagccatag aaaacaccat agagaacatg tatctacaag atctggataa
     7621 tagtacaaaa ctgtatggga aaactggtgc aggattcaca gcaaatagaa ccttacaaaa
     7681 cggatggttt gaagggttta ttataagcaa atcaggacat aaatatgttt ttgtgtccgc
     7741 acttacagga aacttggggt cgaatttaac atcaagcata aaagccaaga aaaatgcgat
     7801 caccattcta aacacactaa atttataaaa aatctaatgg caaaatcgcc caacccttca
     7861 atcaagtcgg gacggccaaa agcaagcttt tggctcccct cgctggcgct cggcgcccct
     7921 tatttcaaac gttaaacatc atgagggaag tggtgatcgc cgaagtatcg actcaactat
     7981 cagaggtagt t
//
LOCUS       DAZPCS010000523         7053 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.guided.180, whole genome shotgun sequence.
ACCESSION   DAZPCS010000523 DAZPCS010000000
VERSION     DAZPCS010000523.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7053
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.guided.180"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            59..1102
                     /locus_tag="ACHUQ7_004493"
     CDS             59..1102
                     /locus_tag="ACHUQ7_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFR1137536.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCQGRPD"
     gene            1164..1778
                     /gene="prfH"
                     /locus_tag="ACHUQ7_004494"
                     /pseudo
     CDS             1164..1778
                     /gene="prfH"
                     /locus_tag="ACHUQ7_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(1835..3292)
                     /gene="pepD"
                     /locus_tag="ACHUQ7_004495"
     CDS             complement(1835..3292)
                     /gene="pepD"
                     /locus_tag="ACHUQ7_004495"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFR1137537.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3554..4012
                     /gene="gpt"
                     /locus_tag="ACHUQ7_004496"
     CDS             3554..4012
                     /gene="gpt"
                     /locus_tag="ACHUQ7_004496"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFR1137538.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            4104..5348
                     /gene="frsA"
                     /locus_tag="ACHUQ7_004497"
     CDS             4104..5348
                     /gene="frsA"
                     /locus_tag="ACHUQ7_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFR1137539.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            5406..5807
                     /gene="crl"
                     /locus_tag="ACHUQ7_004498"
     CDS             5406..5807
                     /gene="crl"
                     /locus_tag="ACHUQ7_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFR1137540.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(5846..6901)
                     /gene="phoE"
                     /locus_tag="ACHUQ7_004499"
     CDS             complement(5846..6901)
                     /gene="phoE"
                     /locus_tag="ACHUQ7_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFR1137541.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
BASE COUNT         1716 a         1735 c         1817 g         1785 t
ORIGIN      
        1 aattcaagtc gcagcacatg caacttgaag tatgacgagt ctatttacgg aatggtatat
       61 gggcaattat attcgtccct tatccgatgc ggtatttacc atcgcatctg atgacctgtg
      121 gatcgagagt ttagcgatcc aacaattaca caccacggca aatttaccca acatgcagcg
      181 cgtagtgggg atgccagatt tacaccccgg acgcggctac ccgattggcg cagcgttctt
      241 ctccgcaggc cgtttttacc cagcactggt cggcaatgat atcggctgtg gcatggcgct
      301 atggcaaaca gatattctcg ctcgcaaata caacgccgat aagtttgaaa agcgattatc
      361 agctttggat gacgttgctg aagaaagctg gctggaggaa aacctgccgt cagcatttgc
      421 acagcatccg tggcgcagct cgcttggctc tatcggtggt ggcaatcatt tcgctgaact
      481 gcaacaagtt gatcaaatca tcaacgctga actgtttgca ctggcaggtc tggatgcgca
      541 gcatctgcaa ctgctggttc atagcggctc gagggggcta gggcagtcta ttttacagcg
      601 gcatattgcc tcgttttcgc atcatggttt gcctgaaggc agtgacgacg cgctggcgtt
      661 tgcgcgtttt aatcgccacc tgatcgcttt gcgcataatg caacaggtta aggccaccgg
      721 aagtccggtt ctggatgtgg cgcataactt tgttagcgcg tgtcgaatcg gtgatcaaca
      781 aggggtctta catcgtaaag gtgccacgcc tgatgactgt ggtctggtgg ttattcccgg
      841 ttcacgcggt gattactcca ggctggttca gcccgtcagg agtgaggaaa cattgcattc
      901 gctggcgcat ggggctgggc gtaaatgggg gcgcaccgag tgtaaagggc gtctggcagc
      961 gaaatacaca gcgacgcaac tctcgcgtac tgaacttggc agtcgggtaa tttgtcgcga
     1021 taaacaactc atcttcgaag aagcgccaca agcctataaa tcggctgaaa gtgtggtgca
     1081 atgtcagggc aggccggatt aattattcct gtcgcgcggc tgcgtccggt gctaacgctc
     1141 aaaaacagtg gagggaaaaa aggatgatct tgctataact ctcctcagcg caggggccgg
     1201 aagaatgttg tctcgcagtg aaaaaggcgc tggacaggct aattaaagaa gctgcccgac
     1261 aagacgtcgc ggtaacggtg ctggaaacag aaacaggccg ctactctgac acgctgcgtt
     1321 cggcgctgat ttctctggat ggcgacaacg catgggcgtt aagcgaaagc tggtgtggca
     1381 ctattcagtg gatttgcccg agcccgtatc gcccacatca cgggcgtaaa aactggtttc
     1441 tgggcattgg gcgttttacc gctgatgagc aggagcaatc cgatgcgatc cggtatgaga
     1501 cgctgcgttc gtcggggccg ggcggtcaac atgtcaataa aaccgactcg gcggtacgcg
     1561 ccacgcattt ggcatccggt attagcgtga aggttcagtc agagcgcagc cagcatgcta
     1621 acaaacggct ggcgcgattg ctgattgcct ggaagctgga tcagcagcaa taggaaaata
     1681 gcgcggtgct gaaatcacag cggcgaatgt tccatcacca gattgaacgt ggcaacccgc
     1741 gacgaacgtt tacagggatg gcttttatcg aaggataatg aaggatgaaa ctgccggaag
     1801 gcgattaaac gccatccggc agcaaatcaa ataattactt cgccggaatt tctttcagca
     1861 gttcagtcag cagtgtccag taatgaccta cgctttcgat gtgaacttgc tcatccggag
     1921 agtgtggacc ggtgatagtt ggcccgatag aaaccatgtc catttccgga tacggttttt
     1981 tgaacagacc acattccagg cccgcgtgga taatctggat gttcggcgtc ttgttgaaca
     2041 ggcgctgata ggtttcacgt accagatgca tcaccggaga attagcgtcc ggctgccagc
     2101 caggatatgc gcctttcgct tcggttttcg cgccagccag tttacccagc gaatccagca
     2161 tgctcaccac gtagtcttta ccgctgtcga ttagtgaacg gatcaggcag tgaatttcta
     2221 cgttattgtc agtcatggtc accacaccga cgttcaggga ggtttcgacc acaccttttg
     2281 ccacgtcgga attgcggatc acaccgttcg gggtggcgtt cagcagacga ataaaggtat
     2341 cgcgagattt cgcaatcagg gcagctttat cgttcgctac agagtccagc aacaaggcca
     2401 gatttttctc tttctctgcc agctcgtttt tcaggatctc ctgataggta ttcaccagag
     2461 atttcagtgc gtcgacttta tcagctgcga cagcaatggt cgcaaaggct tcacgcggga
     2521 tggcgttacg cagtgtgccg ccgttgaaat cgataaggcg caggtccagt tcttctgcat
     2581 gacccgccag gaagcgcacc agcagtttgt tggcattacc caggccaacg tggatttcac
     2641 cgccggagtg accgcctttc agacctttta aggttaactt gaaggtttca aaaccagctg
     2701 gaaccgcttc acgatctaaa tgcaggttgg aggtgaagtc gatacccccc gcacaaccca
     2761 tgtagatttc accttcttct tcggagtcgg tgttaatcag aatatcagcc tgcaaccagt
     2821 tgctctgtaa gccgaacgca ccgtccatac cggcttcttc ggtcatggtc agcagcactt
     2881 ccagcgggcc gtgaaccacg ttttcgtcag ccagaaccgc cagtgcagag gccataccaa
     2941 tgccgttatc cgcacccagc gtggtgccgc gcgctttaac ccattcgcca tcaatataag
     3001 gctggatagg atctttcgtg aagtcatgca cggtgtcgtt atttttctgc ggcaccatat
     3061 cgaggtgggc ctgtaagacg accggtttac gattttccat acctgcggta gcaggttgac
     3121 gaatcaggat attacctacc tgatcgcgtt cgacatggaa acctttctct tttgcccaac
     3181 caacaatgta ttcagcgagt tgctcttcat gataggacgg gtgaggaata gaacagattt
     3241 tggcaaaaat atcccacagc ggctgtggag ataattgaga cagttcagac acgttaagtc
     3301 tccttgtcga tcacccgcaa aacagtattg caggtcacag ggttagcaga aaatgttgtc
     3361 aacacaagac aggcttgcga gatatgtatt gagaatacca ctttatcccg cgtcagggag
     3421 aggcagtgcg taaaaagacg cggactcatg tgaaatactg gtttttagtg cgccagatct
     3481 ctataatctc gcgcaaccta ttttcccctc gaacactttt taagccgtag ataaacaggc
     3541 tgggacactt cacatgagcg aaaaatacat cgtcacctgg gacatgttgc agatccatgc
     3601 acgtaaactc gcaagccgac tgatgccttc tgaacaatgg aaaggcatta ttgccgtaag
     3661 ccgtggcggt ctggtaccgg gggcgttact ggcgcgtgaa ctgggtattc gtcatgtcga
     3721 taccgtttgt atttccagct acgatcacga caaccagcgc gagcttaaag tgctgaaacg
     3781 cgcagaaggc gatggcgaag gcttcatcgt tattgatgac ctggtggata ccggtggtac
     3841 tgctgttgcg attcgtgaaa tgtatccaaa agcgcacttt gtcaccatct tcgcaaaacc
     3901 ggctggtcgt ccgctggttg atgactatgt tgttgatatc ccgcaagata cctggatcga
     3961 acagccgtgg gatatgggcg tcgtattcgt cccgccaatc tccggtcgct aatcttttca
     4021 acgcctggca ttgccgggcg ttgttctttt taacttcagg caggttacaa tagtttccag
     4081 taagtattct ggaggctgca tccatgacac aggcaaacct gagcgaaacc ctgttcaaac
     4141 cccgctttaa acatcctgaa acctcgacgc tagtccgccg ctttaatcac ggcgcacaac
     4201 cgcctgtgca gtcggctctt gatggtaaaa ccatccctca ctggtatcgc atgattaacc
     4261 gtctgatgtg gatctggcgc ggtattgacc cacgtgaaat cctcgacgtc caggcacgta
     4321 ttgtgatgag cgatgctgaa cgtaccgacg atgatttata cgataccgtg attggctacc
     4381 gtggtggcaa ctggatttat gagtgggcca cccaggcgat ggtgtggcaa caaaaagcct
     4441 gtacggaaga agatccgcaa ctcagtggtc gacactggct gcatgcggct acgttgtaca
     4501 acattgccgc ctatcctcat ctgaaaggag atgacctggc cgagcaagcg caggctttat
     4561 caaaccgcgc ctatgaagag gccgcccagc gtctgccggg cacgatgcgg cagatggagt
     4621 ttaccgtacc cggcggtgcg cccatcaccg gctttttgca tatgccgaaa ggcgatggcc
     4681 cgttcccgac agtattaatg tgtggtggtc tggatgcgat gcagacggac tattacagcc
     4741 tgtatgaacg ttattttgcg ccgcgcggca ttgcgatgct gactattgat atgccgtcgg
     4801 tgggcttttc ttcaaaatgg aagctcaccc aggactccag cctgttgcat cagcacgtct
     4861 taaaggcgct gcctaacgta ccgtgggtgg atcacactcg cgtcgcggcc tttggtttcc
     4921 gtttcggcgc taacgttgcc gtgcgtctgg cataccttga atcgccgcgt ctgaaagcgg
     4981 ttgcctgtct tggtccggta gttcataccc tgttgagtga ttttaagtgc cagcaacagg
     5041 tgccggaaat gtatcttgac gttctggcga gtcgtttggg gatgcatgat gcttccgacg
     5101 aagcgttacg cgtggagctg aatcgctatt cattaaaagt gcaaggattg ctggggcgtc
     5161 gctgcccaac gccaatgtta tcaggctact ggaagaacga tccgttcagc ccggaagagg
     5221 actcacgctt aatcacctca tcatctgctg acggtaaatt attagagatc ccatttaacc
     5281 cggtgtatcg gaattttgac aaaggtcttc aggaaatcac cggctggatc gaaaaacgct
     5341 tgtgttaaaa atttgctaaa ttttgccaat ttggtaaaac agttgcatca caacaggaga
     5401 tagcaatgac gttaccgagt ggacacccga agagcagatt gatcaaaaaa tttaccgcac
     5461 taggcccgta tattcgtgaa ggtaagtgcg aagataatcg attctttttc gattgtctgg
     5521 ctgtatgcgt caacgtgaaa ccggcaccgg aagtgcgtga attctggggc tggtggatgg
     5581 agctggaagc gcaggaatcc cgttttacct acagctacca gtttggtctg ttcgataaag
     5641 caggcgactg gaagagtgtt ccggtaaaag atactgaagt ggttgaacga ctggagcaca
     5701 ccctgcgtga gtttcacgag aagctgcgtg aactgctgac gacgctgaat ctgaagctgg
     5761 aaccggcgaa tgattttcgt gatgaaccgg tgaagttaac ggcgtgagtg aaatgtgccg
     5821 gatgcatcac atccggcaat attcattaaa actgatacgt catcccaacc gcgacagtat
     5881 catcattatt aatattcagc ttattatcgc tatccagttg gttgatttta taatcaacaa
     5941 acgctgacat atttttgttg aaataatacg tagcaccgac gtcgatataa ttgaccagat
     6001 cttcatcacc gatgccctca atatctttcc ctttcgataa gacataaccc agcgatggac
     6061 gcagaccaaa gtcaaactgg tattgagcga ccgcttcaaa gttctgtgtc ttattggcaa
     6121 agccgccagt tattggcgtc attttgcgtg tttcagaata gaaagttgcc agataaatat
     6181 tattggcatc gtatttcaga cctgttgccc atgcttctgc acgcttgcct gtgccacggc
     6241 tttgcaggtt ctgctcgttg gtgcgatctg agttggtata ggccccacta atggcgaaat
     6301 cgctgccgcc aaagtcatat gtcaatgacg tgccgaagcc atcgccgttt tgctttttaa
     6361 cgtcgcggtt ttcgtttttc ccttgatatt gcagggttaa gttcaggcca tcgataacgc
     6421 cgaagaagtc ggtgttccga tacgtcgcca gaccgctggc gcgtttggtc ataaagttgt
     6481 cggtctgcgc cgaggaatcg ccaccaaatt ccgggaacat atcggtccag gcttccacgt
     6541 catacaacgc gcccaggtta cgaccataat cgaaagaacc caaatcttta tatttcaacc
     6601 cggcaaaagc gagacgcgtt ttttgctgtg cagtatcact ctccgcttta tttccggcaa
     6661 actccgcttc ccaccgacca taaccagtca gttgatcgtt aatttgagtt tcacctttaa
     6721 aaccaaaacg gatataactc tggtcgccat ctttactggc gttatcactc atataatgca
     6781 tggctttaac tttgccatag acatccagtt tattaccgtc tttattatat atttctgcag
     6841 cctgtacaga tgcagatgcc acaatgccca tcaccactaa tgccagagtg ctctttttca
     6901 ttttcattcc tgattttaat taacgcgcga atattcagcg ggagagcccc gttgaaaaca
     6961 ggaaagtttt taacctgaga ttgttaaaga tatattacag attaatgata ttctgaaaat
     7021 gggtaatgct gccaacttac tgatttagtg tat
//
LOCUS       DAZPCS010000524         4295 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.guided.228, whole genome shotgun sequence.
ACCESSION   DAZPCS010000524 DAZPCS010000000
VERSION     DAZPCS010000524.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4295
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.guided.228"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            558..797
                     /locus_tag="ACHUQ7_004500"
     CDS             558..797
                     /locus_tag="ACHUQ7_004500"
                     /inference="COORDINATES: protein motif:HMM:NF020425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA family protein"
                     /protein_id="HFR1137542.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
     gene            complement(985..1962)
                     /gene="intI2"
                     /locus_tag="ACHUQ7_004501"
                     /pseudo
     CDS             complement(985..1962)
                     /gene="intI2"
                     /locus_tag="ACHUQ7_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            2294..2767
                     /gene="dfrA1"
                     /locus_tag="ACHUQ7_004502"
     CDS             2294..2767
                     /gene="dfrA1"
                     /locus_tag="ACHUQ7_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HFR1137543.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            2862..3386
                     /gene="sat2"
                     /locus_tag="ACHUQ7_004503"
     CDS             2862..3386
                     /gene="sat2"
                     /locus_tag="ACHUQ7_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HFR1137544.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            3444..4282
                     /gene="aadA1"
                     /locus_tag="ACHUQ7_004504"
                     /pseudo
     CDS             3444..4282
                     /gene="aadA1"
                     /locus_tag="ACHUQ7_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048608579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
BASE COUNT         1207 a          943 c         1000 g         1145 t
ORIGIN      
        1 gatcggtcaa ctgatcctta actgatcggc attacaccgg tcggcagctg tgggcggaca
       61 aaatagttgg gaactgggag gggtggaaat ggagttttta aggattattt agggaagagt
      121 gacaaaatag atgggaactg ggtgtagcgt cgtaagctaa tacgaaaatt aaaaatgaca
      181 aaatagtttg gaactagatt tcacttatct ggttaactaa tctagctaac ctgccgctca
      241 gcttagtacg atcctatatc caactgatca taatcctttc atcagtatcc caaattaaat
      301 ccccctccta gactgcctca ccaacacttt acagtgatcc cgtatttccg gacaggtgat
      361 tgattttttt cgcttgtaca ggtgttaagc cacagttatc ttgatgtgga cattatgcat
      421 tgtaatagcc cattaacatg cgctaagagc ataaattaat atacagtaac aagcattacg
      481 aggtgctaat gtttggaaag cagcctcttc caactacaat aataagttaa ctatcctcca
      541 cccacaaata attacaaatg acccctttcc gctataactc cgatctcacc agcggctcgc
      601 tacaaacccg agagtgtcgc attattactg gcttgttact ccaagagctt gatgaagctg
      661 cctgggataa agccatgtat aaggaaaatg tgcttcagaa acgcacgcaa tctacggtaa
      721 gacgtatttc ttcagcgctt agaaaacgcc tagaacacct aagctctgac ttttgggctt
      781 ttgcgttttt atgttaggca ttgtctggca actggaggaa gtgtaatgac catgaatggt
      841 cactgtgatt gacagtatct tgcttaaact tatctgaaca atggtagaac tggctcatat
      901 ttatcccctc tatgattgtt tatctttttc accataaaaa gtatcagggg ctactcaggg
      961 ctacataaag atatttatct acttttactg attgataagt agcatcagtc catccgcagg
     1021 actggtggtg ccggcaaaat gctgacccaa cacatgcgta tagatttgcg tggtcttaac
     1081 atcgttatgc cctaagagtt cttgcacagt gcgaatatca cgccccgctt gtaatagatg
     1141 cgtagcaaac gagtgacgaa atgtatgaca agtgacacgc ttgctaacga tgcctgcttt
     1201 ttgtacggct gccttcaatg cctttcgcgc aacggagtca tgcagatgat ggcggcataa
     1261 tttgccgtta tacgggtggt tgcagagcgt gctggaggga aagacaaaca tccacgccgc
     1321 ttgtcgataa gcagaagggt atttgtgatc taaagcaaaa ggcagcgatg gccctacgcc
     1381 ttgtaagttg tcgtcttgct gaataagccg cgcttgctca atgagttatt ttattgctgg
     1441 gattaggcgc gtgggcagta ggctgtttct gcttttccca cccttaccgt catgcacagt
     1501 gatgcagcca ttatcaaaat caaaatcttt aacccgcaaa cgcaagcatt cattaatgcg
     1561 caaacctgca ccatacagca gcgtaaaaat aacttggttg cgagtatcca taacctgcaa
     1621 aatgcgttgc acttcatttg cagagataac agagggtagc cgtctaggct tgcttgcagg
     1681 gatataatca atatcgccca acggctgttg taaaaacctg ttgtacaaaa aagctagggc
     1741 atttaaagcg attttctgcg tgtttatggc tacatgtctg ctgtttgcta agctggataa
     1801 aaacagcctg acctcttcac tgcccatggt ctgaggatga cgttttttgt gaaacagaat
     1861 aaaacgctta atccagtgca ggtaagtttt ttcagttttc agcgcataac ctttttgtcg
     1921 catatccgtg cgtatagaat ttaaaaatgg actgttagac ataaaacgct ccttgtcttg
     1981 caactgtctg cctatacagc ctattctagc tgggatttaa aaaagtgcct gttttttacg
     2041 cctagagatg cttgtttacc ggtagagttt taatttaatg ctaaataaat taaaatgtta
     2101 tgagttcttt gggtgagata atgtgcatcg tgcaagcagg atagacggca tgcacgattt
     2161 gtaataacag agtgtcttgt atttttaaag aaagtctatt taatacaagt gattatatta
     2221 attaacggta agcatcagcg ggtgacaaaa cgagcatgct tactaataaa atgttaacct
     2281 ctgaggaaga attgtgaaac tatcactaat ggtagctata tcgaagaatg gagttatcgg
     2341 gaatggccct gatattccat ggagtgccaa aggtgaacag ctcctgttta aagctattac
     2401 ctataaccaa tggctgttgg ttggacgcaa gacttttgaa tcaatgggag cattacccaa
     2461 ccgaaagtat gcggtcgtaa cacgttcaag ttttacatct gacaatgaga acgtattgat
     2521 ctttccatca attaaagatg ctttaaccaa cctaaagaaa ataacggatc atgtcattgt
     2581 ttcaggtggt ggggagatat acaaaagcct gatcgatcaa gtagatacac tacatatatc
     2641 tacaatagac atcgagccgg aaggtgatgt ttactttcct gaaatcccca gcaattttag
     2701 gccagttttt acccaagact tcgcctctaa cataaattat agttaccaaa tctggcaaaa
     2761 gggttaacaa gtggcagcaa cggattcgca aacctgtcac gccttttgta ccaaaagccg
     2821 cgccaggttt gcgatccgct gtgccaggcg ttaggcgtca tatgaagatt tcggtgatcc
     2881 ctgagcaggt ggcggaaaca ttggatgctg agaaccattt cattgttcgt gaagtgttcg
     2941 atgtgcacct atccgaccaa ggctttgaac tatctaccag aagtgtgagc ccctaccgga
     3001 aggattacat ctcggatgat gactctgatg aagactctgc ttgctatggc gcattcatcg
     3061 accaagagct tgtcgggaag attgaactca actcaacatg gaacgatcta gcctctatcg
     3121 aacacattgt tgtgtcgcac acgcaccgag gcaaaggagt cgcgcacagt ctcatcgaat
     3181 ttgcgaaaaa gtgggcacta agcagacagc tccttggcat acgattagag acacaaacga
     3241 acaatgtacc tgcctgcaat ttgtacgcaa aatgtggctt tactctcggc ggcattgacc
     3301 tgttcacgta taaaactaga cctcaagtct cgaacgaaac agcgatgtac tggtactggt
     3361 tctcgggagc acaggatgac gcctaacaat tcattcaagc cgacaccgct tcgcggcgcg
     3421 gcttaattca ggagttaaac atcatgaggg aagcggtgat cgccgaagta tcgactcaac
     3481 tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt
     3541 tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta
     3601 cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa
     3661 cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc
     3721 acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc
     3781 agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta
     3841 tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac
     3901 tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat
     3961 ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca
     4021 tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa
     4081 tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg
     4141 gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gttcactacg
     4201 tgaaaggcga gatcaccaag gtagtcggca aataatgtct aacaattcgt tcaagccgac
     4261 gccgcttcgc ggcgcggctt aactcaagcg ttaga
//
LOCUS       DAZPCS010000525         4292 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.guided.229, whole genome shotgun sequence.
ACCESSION   DAZPCS010000525 DAZPCS010000000
VERSION     DAZPCS010000525.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4292
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.guided.229"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            558..797
                     /locus_tag="ACHUQ7_004505"
     CDS             558..797
                     /locus_tag="ACHUQ7_004505"
                     /inference="COORDINATES: protein motif:HMM:NF020425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA family protein"
                     /protein_id="HFR1137545.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
     gene            complement(985..1962)
                     /gene="intI2"
                     /locus_tag="ACHUQ7_004506"
                     /pseudo
     CDS             complement(985..1962)
                     /gene="intI2"
                     /locus_tag="ACHUQ7_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            2294..2767
                     /gene="dfrA1"
                     /locus_tag="ACHUQ7_004507"
     CDS             2294..2767
                     /gene="dfrA1"
                     /locus_tag="ACHUQ7_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HFR1137546.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            2862..3386
                     /gene="sat2"
                     /locus_tag="ACHUQ7_004508"
     CDS             2862..3386
                     /gene="sat2"
                     /locus_tag="ACHUQ7_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HFR1137547.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            3444..4279
                     /gene="aadA1"
                     /locus_tag="ACHUQ7_004509"
                     /pseudo
     CDS             3444..4279
                     /gene="aadA1"
                     /locus_tag="ACHUQ7_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048608579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
BASE COUNT         1204 a          943 c         1000 g         1145 t
ORIGIN      
        1 gatcggtcaa ctgatcctta actgatcggc attacaccgg tcggcagctg tgggcggaca
       61 aaatagttgg gaactgggag gggtggaaat ggagttttta aggattattt agggaagagt
      121 gacaaaatag atgggaactg ggtgtagcgt cgtaagctaa tacgaaaatt aaaaatgaca
      181 aaatagtttg gaactagatt tcacttatct ggttaactaa tctagctaac ctgccgctca
      241 gcttagtacg atcctatatc caactgatca taatcctttc atcagtatcc caaattaaat
      301 ccccctccta gactgcctca ccaacacttt acagtgatcc cgtatttccg gacaggtgat
      361 tgattttttt cgcttgtaca ggtgttaagc cacagttatc ttgatgtgga cattatgcat
      421 tgtaatagcc cattaacatg cgctaagagc ataaattaat atacagtaac aagcattacg
      481 aggtgctaat gtttggaaag cagcctcttc caactacaat aataagttaa ctatcctcca
      541 cccacaaata attacaaatg acccctttcc gctataactc cgatctcacc agcggctcgc
      601 tacaaacccg agagtgtcgc attattactg gcttgttact ccaagagctt gatgaagctg
      661 cctgggataa agccatgtat aaggaaaatg tgcttcagaa acgcacgcaa tctacggtaa
      721 gacgtatttc ttcagcgctt agaaaacgcc tagaacacct aagctctgac ttttgggctt
      781 ttgcgttttt atgttaggca ttgtctggca actggaggaa gtgtaatgac catgaatggt
      841 cactgtgatt gacagtatct tgcttaaact tatctgaaca atggtagaac tggctcatat
      901 ttatcccctc tatgattgtt tatctttttc accataaaaa gtatcagggg ctactcaggg
      961 ctacataaag atatttatct acttttactg attgataagt agcatcagtc catccgcagg
     1021 actggtggtg ccggcaaaat gctgacccaa cacatgcgta tagatttgcg tggtcttaac
     1081 atcgttatgc cctaagagtt cttgcacagt gcgaatatca cgccccgctt gtaatagatg
     1141 cgtagcaaac gagtgacgaa atgtatgaca agtgacacgc ttgctaacga tgcctgcttt
     1201 ttgtacggct gccttcaatg cctttcgcgc aacggagtca tgcagatgat ggcggcataa
     1261 tttgccgtta tacgggtggt tgcagagcgt gctggaggga aagacaaaca tccacgccgc
     1321 ttgtcgataa gcagaagggt atttgtgatc taaagcaaaa ggcagcgatg gccctacgcc
     1381 ttgtaagttg tcgtcttgct gaataagccg cgcttgctca atgagttatt ttattgctgg
     1441 gattaggcgc gtgggcagta ggctgtttct gcttttccca cccttaccgt catgcacagt
     1501 gatgcagcca ttatcaaaat caaaatcttt aacccgcaaa cgcaagcatt cattaatgcg
     1561 caaacctgca ccatacagca gcgtaaaaat aacttggttg cgagtatcca taacctgcaa
     1621 aatgcgttgc acttcatttg cagagataac agagggtagc cgtctaggct tgcttgcagg
     1681 gatataatca atatcgccca acggctgttg taaaaacctg ttgtacaaaa aagctagggc
     1741 atttaaagcg attttctgcg tgtttatggc tacatgtctg ctgtttgcta agctggataa
     1801 aaacagcctg acctcttcac tgcccatggt ctgaggatga cgttttttgt gaaacagaat
     1861 aaaacgctta atccagtgca ggtaagtttt ttcagttttc agcgcataac ctttttgtcg
     1921 catatccgtg cgtatagaat ttaaaaatgg actgttagac ataaaacgct ccttgtcttg
     1981 caactgtctg cctatacagc ctattctagc tgggatttaa aaaagtgcct gttttttacg
     2041 cctagagatg cttgtttacc ggtagagttt taatttaatg ctaaataaat taaaatgtta
     2101 tgagttcttt gggtgagata atgtgcatcg tgcaagcagg atagacggca tgcacgattt
     2161 gtaataacag agtgtcttgt atttttaaag aaagtctatt taatacaagt gattatatta
     2221 attaacggta agcatcagcg ggtgacaaaa cgagcatgct tactaataaa atgttaacct
     2281 ctgaggaaga attgtgaaac tatcactaat ggtagctata tcgaagaatg gagttatcgg
     2341 gaatggccct gatattccat ggagtgccaa aggtgaacag ctcctgttta aagctattac
     2401 ctataaccaa tggctgttgg ttggacgcaa gacttttgaa tcaatgggag cattacccaa
     2461 ccgaaagtat gcggtcgtaa cacgttcaag ttttacatct gacaatgaga acgtattgat
     2521 ctttccatca attaaagatg ctttaaccaa cctaaagaaa ataacggatc atgtcattgt
     2581 ttcaggtggt ggggagatat acaaaagcct gatcgatcaa gtagatacac tacatatatc
     2641 tacaatagac atcgagccgg aaggtgatgt ttactttcct gaaatcccca gcaattttag
     2701 gccagttttt acccaagact tcgcctctaa cataaattat agttaccaaa tctggcaaaa
     2761 gggttaacaa gtggcagcaa cggattcgca aacctgtcac gccttttgta ccaaaagccg
     2821 cgccaggttt gcgatccgct gtgccaggcg ttaggcgtca tatgaagatt tcggtgatcc
     2881 ctgagcaggt ggcggaaaca ttggatgctg agaaccattt cattgttcgt gaagtgttcg
     2941 atgtgcacct atccgaccaa ggctttgaac tatctaccag aagtgtgagc ccctaccgga
     3001 aggattacat ctcggatgat gactctgatg aagactctgc ttgctatggc gcattcatcg
     3061 accaagagct tgtcgggaag attgaactca actcaacatg gaacgatcta gcctctatcg
     3121 aacacattgt tgtgtcgcac acgcaccgag gcaaaggagt cgcgcacagt ctcatcgaat
     3181 ttgcgaaaaa gtgggcacta agcagacagc tccttggcat acgattagag acacaaacga
     3241 acaatgtacc tgcctgcaat ttgtacgcaa aatgtggctt tactctcggc ggcattgacc
     3301 tgttcacgta taaaactaga cctcaagtct cgaacgaaac agcgatgtac tggtactggt
     3361 tctcgggagc acaggatgac gcctaacaat tcattcaagc cgacaccgct tcgcggcgcg
     3421 gcttaattca ggagttaaac atcatgaggg aagcggtgat cgccgaagta tcgactcaac
     3481 tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt
     3541 tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta
     3601 cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa
     3661 cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc
     3721 acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc
     3781 agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta
     3841 tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac
     3901 tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat
     3961 ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca
     4021 tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa
     4081 tggagcgcct gccggcccag tatcagcccg tcatacttga agctaggcag gcttatcttg
     4141 gacaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtt cactacgtga
     4201 aaggcgagat caccaaggta gtcggcaaat aatgtctaac aattcgttca agccgacgcc
     4261 gcttcgcggc gcggcttaac tcaagcgtta ga
//
LOCUS       DAZPCS010000526         3570 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.guided.251, whole genome shotgun sequence.
ACCESSION   DAZPCS010000526 DAZPCS010000000
VERSION     DAZPCS010000526.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3570
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.guided.251"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            41..430
                     /locus_tag="ACHUQ7_004510"
                     /pseudo
     CDS             41..430
                     /locus_tag="ACHUQ7_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(408..1031)
                     /gene="tetR(B)"
                     /locus_tag="ACHUQ7_004511"
     CDS             complement(408..1031)
                     /gene="tetR(B)"
                     /locus_tag="ACHUQ7_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HFR1137548.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            1113..2318
                     /gene="tet(B)"
                     /locus_tag="ACHUQ7_004512"
     CDS             1113..2318
                     /gene="tet(B)"
                     /locus_tag="ACHUQ7_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HFR1137549.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(2431..3024)
                     /gene="tetC"
                     /locus_tag="ACHUQ7_004513"
     CDS             complement(2431..3024)
                     /gene="tetC"
                     /locus_tag="ACHUQ7_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HFR1137550.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            3112..3498
                     /locus_tag="ACHUQ7_004514"
                     /pseudo
     CDS             3112..3498
                     /locus_tag="ACHUQ7_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014656611.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          974 a          654 c          734 g         1208 t
ORIGIN      
        1 tctgaacata aaacactatc aataagttgg agtcattacc aaccatggcg tacatgccaa
       61 agtttccacg ccagtgcatt tacgaaaagc acgtacttgc tatgatcatt tagctggcga
      121 agttgccgtt aagatctatg attccctttg tcaacagcaa tggatcactg aaaatggttc
      181 aatgatcaca ttaagtggta ttcaatattt tcatgaaatg ggaattgacg ttccttccaa
      241 acattcacgt aaaatctgtt gtgcgtgttt agattggagt gaacgccgtt tccatttagg
      301 tgggtacgtt ggagccgcat tattttcgct ttatgaatct aaagggtggt taactcgaca
      361 tcttggttac cgtgaagtta ccatcacgga aaaaggttat gctgctttta agacccactt
      421 tcacatttaa gttgtttttc taatccgcat atgatcaatt caaggccgaa taagaaggct
      481 ggctctgcac cttggtgatc aaataattcg atagcttgtc gtaataatgg cggcatacta
      541 tcagtagtag gtgtttccct ttcttcttta gcgacttgat gctcttgatc ttccaatacg
      601 caacctaaag taaaatgccc cacagcgctg agtgcatata atgcattctc tagtgaaaaa
      661 ccttgttggc ataaaaaggc taattgattt tcgagagttt catactgttt ttctgtaggc
      721 cgtgtaccta aatgtacttt tgctccatcg cgatgactta gtaaagcaca tctaaaactt
      781 ttagcgttat tacgtaaaaa atcttgccag ctttcccctt ctaaagggca aaagtgagta
      841 tggtgcctat ctaacatctc aatggctaag gcgtcgagca aagcccgctt attttttaca
      901 tgccaataca atgtaggctg ctctacacct agcttctggg cgagtttacg ggttgttaaa
      961 ccttcgattc cgacctcatt aagcagctct aatgcgctgt taatcacttt acttttatct
     1021 aatctagaca tcattaattc ctaatttttg ttgacactct atcattgata gagttatttt
     1081 accactccct atcagtgata gagaaaagtg aaatgaatag ttcgacaaag atcgcattgg
     1141 taattacgtt actcgatgcc atggggattg gccttatcat gccagtcttg ccaacgttat
     1201 tacgtgaatt tattgcttcg gaagatatcg ctaaccactt tggcgtattg cttgcacttt
     1261 atgcgttaat gcaggttatc tttgctcctt ggcttggaaa aatgtctgac cgatttggtc
     1321 ggcgcccagt gctgttgttg tcattaatag gcgcatcgct ggattactta ttgctggctt
     1381 tttcaagtgc gctttggatg ctgtatttag gccgtttgct ttcagggatc acaggagcta
     1441 ctggggctgt cgcggcatcg gtcattgccg ataccacctc agcttctcaa cgcgtgaagt
     1501 ggttcggttg gttaggggca agttttgggc ttggtttaat agcggggcct attattggtg
     1561 gttttgcagg agagatttca ccgcatagtc ccttttttat cgctgcgttg ctaaatattg
     1621 tcgctttcct tgtggttatg ttttggttcc gtgaaaccaa aaatacacgt gataatacag
     1681 ataccgaagt aggggttgag acgcaatcga attcggtata catcacttta tttaaaacga
     1741 tgcccatttt gttgattatt tatttttcag cgcaattgat aggccaaatt cccgcaacgg
     1801 tgtgggtgct atttaccgaa aatcgttttg gatggaatag catgatggtt ggcttttcat
     1861 tagcgggtct tggtctttta cactcagtat tccaagcctt tgtggcagga agaatagcca
     1921 ctaaatgggg cgaaaaaacg gcagtactgc tcggatttat tgcagatagt agtgcatttg
     1981 cctttttagc gtttatatct gaaggttggt tagttttccc tgttttaatt ttattggctg
     2041 gtggtgggat cgctttacct gcattacagg gagtgatgtc tatccaaaca aagagtcatc
     2101 agcaaggtgc tttacaggga ttattggtga gccttaccaa tgcaaccggt gttattggcc
     2161 cattactgtt tgctgttatt tataatcatt cactaccaat ttgggatggc tggatttgga
     2221 ttattggttt agcgttttac tgtattatta tcctgctatc gatgaccttc atgttaaccc
     2281 ctcaagctca ggggagtaaa caggagacaa gtgcttagtt atttcgtcac caaatgatgt
     2341 tattccgcga aatataatga ccctcttgat aacccaagag ggcatttttt acgataaaga
     2401 agatttagct tcaaataaaa cctatctatt ttatttatct ttcaagctca ataaaaagcc
     2461 gcggtaaata gcaataaatt ggcctttttt atcggcaagc tcttttaggt ttttcgcatg
     2521 tattgcgata tgcataaacc agccattgag taagttttta agcacatcat catcataagc
     2581 tttaagttgg ttctcttgga tcaatttgct gacaatggcg tttaccttac cagtaatgta
     2641 ttcaaggcta attttttcaa gttcattcca accaatgata ggcatcactt cttggatagg
     2701 gataaggttt ttattattat caataatata atcaagataa tgttcaaata tactttctaa
     2761 ggcagaccaa ccatttgtta aatcagtttt tgttgtgatg taggcatcaa tcataattaa
     2821 ttgctgctta taacaggcac tgagtaattg ttttttattt ttaaagtgat gataaaaggc
     2881 acctttggtc accaacgctt ttcccgagat ctcatctatt gaaacagctt gatagccttt
     2941 ttcaacaaac aatattcgtg ctgagttaac cagtgattga taggtactct taaaattttc
     3001 ttgttgatga tttttatttt ccatgataga tttaaaataa cataccgtca gtatgtttat
     3061 ggtatcatga tgatgtggtc gtgacaatct taagaacatt taggttattt tatgtatatt
     3121 gaacagcatt ctcgctatca aaataaagct aataacatcc aattaagata tgatgataag
     3181 cagtttcata caacggttat caaagatgtt ctattatgga ttgaacataa tttagatcag
     3241 tctttactgc ttgatgatgt ggcgaataaa gcgggttata ccaagtggta ttttcagcgg
     3301 ctgttcaaaa aagtaacagg ggtcacactg gctagctata ttcgtgctcg tcgtttgacg
     3361 aaagcggctg ttgagttgag gttgacgaaa aaaactatcc ttgagatcgc attaaaatat
     3421 caatttgatt cccaacaatc ttttacacgt cgatttaagt acatttttaa ggttacacca
     3481 agttattatc ggcgtaataa attatgggaa ttggaggcaa tgcactgaga gggtgatgct
     3541 gccaacttac tgatttagtg tatgatggtg
//
LOCUS       DAZPCS010000527         1361 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.guided.372, whole genome shotgun sequence.
ACCESSION   DAZPCS010000527 DAZPCS010000000
VERSION     DAZPCS010000527.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.guided.372"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..397
                     /locus_tag="ACHUQ7_004515"
     CDS             <1..397
                     /locus_tag="ACHUQ7_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFR1137551.1"
                     /translation="NQDFSGDKERNNGLTEAWLESSLKISPEEQIQFLRKIINHNLPV
                     KNSAIENTIENMYLQDLDNSTKLYGKTGAGFTANRTLQNGWFEGFIISKSGHKYVFVS
                     ALTGNLGSNLTSSIKAKKNAITILNTLNL"
     gene            510..1348
                     /gene="aadA1"
                     /locus_tag="ACHUQ7_004516"
                     /pseudo
     CDS             510..1348
                     /gene="aadA1"
                     /locus_tag="ACHUQ7_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048608579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
BASE COUNT          405 a          317 c          328 g          311 t
ORIGIN      
        1 aaatcaagac ttctctggag ataaagaaag aaacaacgga ttaacagaag catggctcga
       61 aagtagctta aaaatttcac cagaagaaca aattcaattc ctgcgtaaaa ttattaatca
      121 caatctccca gttaaaaact cagccataga aaacaccata gagaacatgt atctacaaga
      181 tctggataat agtacaaaac tgtatgggaa aactggtgca ggattcacag caaatagaac
      241 cttacaaaac ggatggtttg aagggtttat tataagcaaa tcaggacata aatatgtttt
      301 tgtgtccgca cttacaggaa acttggggtc gaatttaaca tcaagcataa aagccaagaa
      361 aaatgcgatc accattctaa acacactaaa tttataaaaa atctaatggc aaaatcgccc
      421 aacccttcaa tcaagtcggg acggccaaaa gcaagctttt ggctcccctc gctggcgctc
      481 ggcgcccctt atttcaaacg ttaaacatca tgagggaagt ggtgatcgcc gaagtatcga
      541 ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg
      601 tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc
      661 tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt
      721 tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg
      781 ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag
      841 aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc
      901 tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg
      961 aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa
     1021 cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt
     1081 cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact
     1141 gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt
     1201 atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgttc
     1261 actacgtgaa aggcgagatc accaaggtag tcggcaaata atgtctaaca attcgttcaa
     1321 gccgacgccg cttcgcggcg cggcttaact caagcgttag a
//
LOCUS       DAZPCS010000528         1358 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2302510
            SAMN43277720-rid23486053.guided.373, whole genome shotgun sequence.
ACCESSION   DAZPCS010000528 DAZPCS010000000
VERSION     DAZPCS010000528.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277720
            Sequence Read Archive: SRR30464137
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396488.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396488.1
            Genome Coverage       :: 203x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:25:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 3,952
            CDSs (with protein)               :: 3,952
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 480
            CDSs (without protein)            :: 480
            Pseudo Genes (ambiguous residues) :: 0 of 480
            Pseudo Genes (frameshifted)       :: 223 of 480
            Pseudo Genes (incomplete)         :: 163 of 480
            Pseudo Genes (internal stop)      :: 161 of 480
            Pseudo Genes (multiple problems)  :: 63 of 480
            Pseudo Genes (short protein)      :: 1 of 480
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277720-rid23486053.guided.373"
                     /strain="M2302510"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-17"
                     /collected_by="FSS"
     gene            <1..397
                     /locus_tag="ACHUQ7_004517"
     CDS             <1..397
                     /locus_tag="ACHUQ7_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFR1137552.1"
                     /translation="NQDFSGDKERNNGLTEAWLESSLKISPEEQIQFLRKIINHNLPV
                     KNSAIENTIENMYLQDLDNSTKLYGKTGAGFTANRTLQNGWFEGFIISKSGHKYVFVS
                     ALTGNLGSNLTSSIKAKKNAITILNTLNL"
     gene            510..1345
                     /gene="aadA1"
                     /locus_tag="ACHUQ7_004518"
                     /pseudo
     CDS             510..1345
                     /gene="aadA1"
                     /locus_tag="ACHUQ7_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048608579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
BASE COUNT          402 a          317 c          328 g          311 t
ORIGIN      
        1 aaatcaagac ttctctggag ataaagaaag aaacaacgga ttaacagaag catggctcga
       61 aagtagctta aaaatttcac cagaagaaca aattcaattc ctgcgtaaaa ttattaatca
      121 caatctccca gttaaaaact cagccataga aaacaccata gagaacatgt atctacaaga
      181 tctggataat agtacaaaac tgtatgggaa aactggtgca ggattcacag caaatagaac
      241 cttacaaaac ggatggtttg aagggtttat tataagcaaa tcaggacata aatatgtttt
      301 tgtgtccgca cttacaggaa acttggggtc gaatttaaca tcaagcataa aagccaagaa
      361 aaatgcgatc accattctaa acacactaaa tttataaaaa atctaatggc aaaatcgccc
      421 aacccttcaa tcaagtcggg acggccaaaa gcaagctttt ggctcccctc gctggcgctc
      481 ggcgcccctt atttcaaacg ttaaacatca tgagggaagt ggtgatcgcc gaagtatcga
      541 ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg
      601 tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc
      661 tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt
      721 tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg
      781 ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag
      841 aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc
      901 tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg
      961 aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa
     1021 cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt
     1081 cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact
     1141 gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct aggcaggctt
     1201 atcttggaca agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgttcact
     1261 acgtgaaagg cgagatcacc aaggtagtcg gcaaataatg tctaacaatt cgttcaagcc
     1321 gacgccgctt cgcggcgcgg cttaactcaa gcgttaga
//
